LOCUS       DAOQOD010000001        86964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_66_length_86964_cov_13.227019, whole genome shotgun sequence.
ACCESSION   DAOQOD010000001 DAOQOD010000000
VERSION     DAOQOD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 86964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86964
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_86964_cov_13.227019"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLZ72_00005"
     CDS             complement(<1..180)
                     /locus_tag="PLZ72_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34710.1"
                     /translation="MSTDPRTLAVLVGRPVEYIDADGRVVRTAFRKQVVDGAVAVGPT
                     NLDGDEQGDLRVHGGP"
     gene            223..540
                     /locus_tag="PLZ72_00010"
     CDS             223..540
                     /locus_tag="PLZ72_00010"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNA34711.1"
                     /translation="MYVVMNSIAVDPEHAEIFEQHFSRSMDGTLGSVGGLVRSTLLRP
                     VTAGEPYVAQITFDSEESFQGWLSSDAFRAAHGHGPSGGSGSSGDGPNVVAYEVVNEV
                     AAG"
     gene            590..673
                     /locus_tag="PLZ72_00015"
     tRNA            590..673
                     /locus_tag="PLZ72_00015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:624..626,aa:Leu,seq:cag)
     gene            1099..1545
                     /locus_tag="PLZ72_00020"
     CDS             1099..1545
                     /locus_tag="PLZ72_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34712.1"
                     /translation="MAVNLDAGSGDRLVDRLQSEFYVPTASARAAVRAQESFNILAVE
                     QGLKADLFVLGDSLLDRRQIERRVLIVLPGSTQGLWVTSPEDQILRKLDWYCRGGSVS
                     DRQWRDVLGLLTVGGDALEVDYLHETAAEVGLSDLLERAVAAASEG"
     gene            1724..2641
                     /locus_tag="PLZ72_00025"
     CDS             1724..2641
                     /locus_tag="PLZ72_00025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA34713.1"
                     /translation="MRIGINGSGLIATGAPVGQITEHAAQAEADGFSSYWLAQLAVPD
                     ALTAIATMGEATSSIEIGTAVVPTWFRHPLMLAAQAVTVQQIIGPRLALGIGLAHKLS
                     VEATLRIPFATPAKHMDEYLSVLLPAMTDGSVDVTGDIWSGFTEGAPPAEGPPTVLLA
                     AMGPRMLQLAGSRCAGTILWLSGPKVIAERIKPAVDAAAAEAGRPTPRIVASVPVCVT
                     DDADGVKEMVGAFLAGYNDLPSYRGVMDAEGAGGPADVSVIGDEATVRAGIAAFAEAG
                     ATDFAPVEIGLAERDQVATRDLLKELAAG"
     gene            complement(2648..3091)
                     /locus_tag="PLZ72_00030"
     CDS             complement(2648..3091)
                     /locus_tag="PLZ72_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNA34714.1"
                     /translation="MSTHVAQVEWHRDGAPTDSTYSRAHVWRFDGGVEVPASSAPHLN
                     GDPARVDPEEAFVAAIASCHMLWFLHLASDAGFPVETYRDDAVATMGRVEDGVLAITD
                     VELHPVITWSGPEPDDAALAQLHRDSHHRCFIANSVRSNITVAGT"
     gene            3201..4214
                     /locus_tag="PLZ72_00035"
     CDS             3201..4214
                     /locus_tag="PLZ72_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34715.1"
                     /translation="MTTSGRTTPALRRLRATVSVVLLLVGVASACSGGGSEEGSARGG
                     NPTDSAATPSSSATPTGTPSTGCGTLPATESVEAPPGDAVVTLGDRSYRLGIPADYDR
                     DRPVPLVMNLHGSGSNALEQTTYSEMAARAGERGWITVTPDAIGGKWELAPTGADDEF
                     LTAVFDQVTAGYCVDLDRVHLTGMSLGAWKAAATACAHADRFASIALVTVEVHPSDCP
                     PMSVVAFHGTADTVVPYGEGADPGVVVTGPNAGLPGARGNMAGWASAAGCGAEPRVQP
                     IGSDVEHWTYPGCPDGRGVEFYSIEGGGHTWPGASIDIGPTTQTIDATDIALDWFEAH
                     PRA"
     gene            complement(4237..5010)
                     /locus_tag="PLZ72_00040"
     CDS             complement(4237..5010)
                     /locus_tag="PLZ72_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNA34716.1"
                     /translation="MLRFSRGVAERRPGAVLVMAVLLVLGFALVVRWHAGRSLAAPQP
                     VAAVAGAVLDRDGPDPAVSSALQRSVLRVHASGCGQERQATVTLVRRADTVVGLTNQH
                     VVAGTDRVDVDGVAGSADVSGVVADRDVAEIDGAALSDGGAEPLEAGPRPVLGSRVMV
                     AGYPDGRFLAASGTVRSVEARQGYGATTEVMVVDVDAVPGISGGVVIDITGRAVGLIA
                     ASDPVTHDVVAYPLDAIGAADPALGAACRPADPAATGAG"
     gene            5108..5650
                     /locus_tag="PLZ72_00045"
     CDS             5108..5650
                     /locus_tag="PLZ72_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34717.1"
                     /translation="MMQFDPAMADLAALRERRLATPDPGARPIIRSITPDDGPALTEM
                     ALRCSPDSIRHRFHAPLTGAEPSRLTALLRGRGAIITLVADVDGAVVGIATLHRSGRG
                     AAEVAVLVEDRWQAGGVGTRLLSHLMRHAAQHGIRTVDADVQREQGFLIDRLLRGVPD
                     AAASFDGPVATLHLPVGPAS"
     gene            5660..6160
                     /locus_tag="PLZ72_00050"
     CDS             5660..6160
                     /locus_tag="PLZ72_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA34718.1"
                     /translation="MRRASFTDMQCSVAQSLEQIGEWWSLLIIRDALLGVRRFEDFRT
                     RLGIASNILTSRLDTLVAHGILERRRYSEHPPRDEYRLTDKGRDLWPVMTAIRQWGDK
                     WALDLEDHPTVLVHDTCGHVCQAVPHCSECGQELELGSIHVAPGPNDSDPSFIPRRPA
                     PGSDRS"
     gene            complement(6180..7172)
                     /gene="meaB"
                     /locus_tag="PLZ72_00055"
     CDS             complement(6180..7172)
                     /gene="meaB"
                     /locus_tag="PLZ72_00055"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNA34719.1"
                     /translation="MAALLPVSDYARGIRAGDRSVLARAITLVESQNPEHRTVAQALV
                     QELLASTGGAHRVGITGVPGVGKSTFIDQLGIDLTAAGHRVAVLAVDPTSSRTGGSIL
                     GDKTRMERLAVDPAAFVRPSPAGRSLGGVTRATRETILLCEAAGFDVVLVETVGVGQS
                     ETVVADMVDFFLVLMLAGAGDELQGIKKGVLELADLVAVNKADGDNRPRAELAAADYR
                     AALHLMSPASPTWSPPVVVCSGLTGDGLDELWQQVVIHRARMSATGELQERRRDQQVR
                     WMWAMLDDRLRDDLRTDPAMGAVLDAARQRVHDGEIPATVAVDEVWRRYLECRS"
     gene            7304..8038
                     /locus_tag="PLZ72_00060"
     CDS             7304..8038
                     /locus_tag="PLZ72_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA34720.1"
                     /translation="MTVRHTTMARRTREERRQDYLDAGVELLTEQTAARPDPGLAFAH
                     VRVADVAARAGVTKGALYHLWDSQEAYWHDLLEFMLDEGRLAGMPAVTDATRELAEAT
                     DDLPTPVEWADHVFERFKDDPSFFARIGMLSYLHDGGVHSDLDEEFRASLAEFEAMVA
                     TSVRQLGRRPRVGTDLRDFSAAVAALMHGFCLEHRNDPDRTPEVVVQGRPQSLFASCI
                     TAFLDAFTEPDTAQEPDTAHESGGNP"
     gene            8038..8724
                     /locus_tag="PLZ72_00065"
     CDS             8038..8724
                     /locus_tag="PLZ72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA34721.1"
                     /translation="MARPTAAERRLDYLRIGADLVTGFGDAGSPAGPLEALANVKVSD
                     VARHAGVTKGALYHLWDSQDDYRRDLLQHLLDQVEKEGLERAEQIIAEAVDDEGRPFI
                     DVTDLAVHLSTGSYRDLRDDPRAQAQFSFYNYVHDPRVRRLLATGSSGFDSYYDLYLV
                     TSGRRMREPYTVGHLITSVNAFMMGSVIRHRISRGSDTSADDAQIYATGFRALLLHFT
                     EPLRDTEPLR"
     gene            complement(8798..11020)
                     /gene="scpA"
                     /locus_tag="PLZ72_00070"
     CDS             complement(8798..11020)
                     /gene="scpA"
                     /locus_tag="PLZ72_00070"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNA34722.1"
                     /translation="MTADGPRFEVPDLSTVDLASSAAADPGARERWAAAAQSATGLAV
                     DEQRWETPEGIDVAPLYSAADLDGLDFLHTYPGLAPFLRGPYPTMYVNQPWTIRQYAG
                     FSTAEDSNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMAGVAIDSIYDM
                     RVLFDGIPLDQMSVSMTMNGAVLPVLSLYIVAAEEQGVAPEKLAGTIQNDILKEFMVR
                     NTYIYPPTPSMRIISDIFSYTSERMPRFNSISISGYHMQEAGATADLELAYTLADGLE
                     YIQAGLDAGLDVDAFAPRLSFFWAIGMNFFMEIAKMRAARLLWAKLVKERFDPGNAKS
                     LSLRTHSQTSGWSLTAQDVFNNVIRTCVEAMASTQGHTQSLHTNSLDEALALPTDFSA
                     RIARNTQLFLQQESGTTRVIDPWGGSYYVERLTAELAQRALTHIAEVDQLGGMAAAIE
                     AGIPKLRIEEAAARTQARIDAGRQPVIGVNKYRLDEHEDIDVLKVDNSAVRAGQIAKL
                     ERLRAERDPEAVRVALEALTNAASSGEGNLLDLGVAAARAKASVGEISDALEKVYGRH
                     RADIRSISGVYRSELGERGAAVDQVRRKVEAFEAADGRRPRILLAKMGQDGHDRGQKV
                     IASAFADLGWDVDIGPLFQTPEEVARQAVEADVHIVGPSSLAAGHLTLVPELKQALEA
                     LGRPDILVVVGGVIPPQDYPALYDAGAAAVFPPGTVIHEAASDLLDRLADQLGYDLST
                     "
     gene            complement(11017..12903)
                     /locus_tag="PLZ72_00075"
     CDS             complement(11017..12903)
                     /locus_tag="PLZ72_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HNA34723.1"
                     /translation="MSEPTTAPTAATALAGGFDPADEEAWLAAVDKVLKGAPFTKLVG
                     RTADGVTVQPLYTPDNSPGAADEAGFPGQAPFTRGTHPAPRPDVAWDIRSVVGGPDPA
                     AANARALTDLERGVTSLEVPWTEATDLDAVLSGVHLDLAPVVLRPGFDFTAAADALIA
                     LWDQRGIDPAQAVGGFGADPLAALAARGGAGQDPQALLTELGALAARTAATHPRVRSV
                     TVSTLPAVEAGASEAQELAIMLGTGAAYLRAMSAAGLSADEAAGQIQLELAADEDVFA
                     TVAKLRAARRLWANLLGAVGVSGDAPAPVLHVVEARRMLTRRDPWVNLLRTTAAVFAA
                     GIGGADGVTAHPYDVLLGDPSDLGRRMARNTQILLQEESNLGRVIDPAGGSGYVESLT
                     DELADLAWRLLQELESVGGMPTVLLDGTLAARIADVRDARLARIATRRAPITGVSEFP
                     DLGEDPPPPPPPEPDGVDPLLVPVRWAERFEELRDRADAARAAGREPVVFLANLGPVA
                     THTARATFAKNLFEAGGLRTVTSGRGAGTGFATAADAAEDFAASGAALVCICSSDAVY
                     AEHAVAAAEALRAAGARRVYLAGNPGDRRAAEEAAGVDEFVHVGVDVLATLERAQEIL
                     EVSS"
     gene            13052..13411
                     /locus_tag="PLZ72_00080"
     CDS             13052..13411
                     /locus_tag="PLZ72_00080"
                     /inference="COORDINATES: protein motif:HMM:NF020873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HNA34724.1"
                     /translation="MDDRPDPIELPDGLELARTTDVFDEANHPGGLLRAHQVAAGVWA
                     RLLVHTGSLRFVFEDESDRPRTVSAGGSQVIPPQRRHHVEFDGPATFAIEFHRPPATA
                     GPAEGPAEGAESTGLQP"
     gene            complement(13454..13993)
                     /locus_tag="PLZ72_00085"
     CDS             complement(13454..13993)
                     /locus_tag="PLZ72_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNA34725.1"
                     /translation="MIHAAGFATEFNTVMLVLRVVAGVTLFLHGYNKVFRGGKIEGTA
                     GWFEGLGMKPGRLNAWMAASTEMGAGVLLTVGLLTPFAAAGLIGIMVVAARTDHRGSF
                     FMFKNGWEYVMVFGFLAWGIGALGPGEWSLDHALGILDDLTRDWRGALIAGVLGLVAG
                     IGTLVVFHHPEPAADSGGS"
     gene            14110..14826
                     /locus_tag="PLZ72_00090"
     CDS             14110..14826
                     /locus_tag="PLZ72_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNA34726.1"
                     /translation="MAELRTFPEDWDRALAVVAHPDDLEYGAASAVARWTAQGKEVIY
                     LLVTDGEAGIDGMAPEECGPLRRDEEIASAAVVGVHTVEFMGRPDGLVVADLDLRRDL
                     AAAIRRHRPDVLIATNFREHWGGEGWNHVDHRNTGLALLDAARDAGNRWLFTDAGEPW
                     SGTRFAAFLGSDMATHGVDVGDWIDKGVESLEAHRTYLDALPPGTPGTDPEPFLRGMA
                     EWAGPRLGVDLAVTFELISI"
     gene            complement(14886..16592)
                     /locus_tag="PLZ72_00095"
     CDS             complement(14886..16592)
                     /locus_tag="PLZ72_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA34727.1"
                     /translation="MHDLIIRAGSVVDGTGSAARTADVAITDGIISEVGRVDGPARRE
                     LDADGLLVTPGFVDIHTHFDGQVTWDPMLTPTCWHGVTTVVMGNCGVGFAPVHPDRHD
                     WLIGLMEGVEDIPGAALSDGIRWSWETFPEYLDAVDRAPKMLDVGTQVPHGAVRAYVM
                     GERGARNEPATADDIEMMAAIVGEAVRAGALGFSTSRTIAHMAIDGEPVPGTFAAEDE
                     LFGIGAALAEAGTGVFELAPAGALGEDLAAPDTEMAWMRRLGAAIDRPIVFALTQNDH
                     DPLAYRRMLELSAEAQDEGSLVIPQVASRPINLLLGLQTFHPFSYCPSWSEVGLLSIA
                     ERVRAMGDPAFRERLLAEVGEMDPAMAQFLDPERVVTMGAVPDYEPSLDQTIGARARA
                     RGISPWELYYDDLLGDDGDALIMRPLLNYSDFNLDAVHEMLSSPVTRWGLGDGGAHCG
                     TTCDASAATFLLTHWARDRADGLPVEQAVRTLTSDTASLYGLGDRGVLAAGRVGDVNL
                     IDLDALSLRRPEMVHDLPGDARRFIQRADGYRATVKSGEVTLVDGEDQGARPGRLLRG
                     AR"
     gene            16809..17348
                     /locus_tag="PLZ72_00100"
     CDS             16809..17348
                     /locus_tag="PLZ72_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNA34728.1"
                     /translation="MTDPSGPDLAGLSASIDRLTDHAHDWSTQRLLDEGLDLVRDACW
                     ADAAALFRVGARRAAAVHARPSVRSRRTRPGGVCAPVPDAVDTGWFPWGLAPLAADRF
                     VLIDDARALPADPDGARTLGELGVRSCLHLPLRQRGRTVGALQVFWSEPRLAWDDDRG
                     RLLRTLGRFLLTCDGRVRT"
     gene            complement(17355..18752)
                     /locus_tag="PLZ72_00105"
     CDS             complement(17355..18752)
                     /locus_tag="PLZ72_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNA34729.1"
                     /translation="MTDLTAGRPPEGSTEPAADPDVEVPQLVLHMGQGRALPRWFWRA
                     IVAVAVSVAAYQFGRSILIQLQGFLLIVAISLFLSFAVEPAVDFLADRGWKRSLATLF
                     CFLALFVVGGLFLFVMGDLVVTQVEDLVDRAPSYLDESADWINNTFDTDITTDDITTQ
                     IESYQDDLTDIAKSLGGRVLSVTGTLFGLLFQAFTIGLFSYYMTSQGPQLRRNVCSIL
                     PQRNQRTVLLLWELAIQKTGGWIYSRLLLAVLSSACTWVFLSIVGVPSPLALALWVGL
                     ISQFIPAIGTYLAGALPVLVALLNDPIDALWVLGFILVYQQVENYLFAPKITAQTMDL
                     HPAVAFGSAIVGGTLLGPMGAIMALPAAGVVQAFVSTVLERHEVVDSHLTEMEEIRAE
                     SGDQGVRRAMRRMLRMGDGDAAVGVRRMDDDGIPLDPATGPDRASSADAADSSDQVAG
                     SVDDRETGPTEGRSD"
     gene            complement(18796..19578)
                     /locus_tag="PLZ72_00110"
     CDS             complement(18796..19578)
                     /locus_tag="PLZ72_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA34730.1"
                     /translation="MSDPRPEPTDHPRAAGTYGPGAFDDRFYFRQLLAGRDFAVGDPV
                     ATQMVNFAYLIGDRVRGECVVVDPAYAVSDLVAVAAADGMTLSGALVTHYHPDHCGGS
                     MMGMTIEGAAELVEVAGVPIHVQSDEAEWVLKVTELTAADLVTHDAGDTVMVGDIPVE
                     LIHTPGHTPGSQCFYVNNCLVSGDTLFLDGCGRTDLPGGDPEALYHSLTGTLARVPDD
                     ALLFPGHQYSVESSDLMGDTRARNVVFKPRSAEQWLMMFGPG"
     gene            complement(19589..20404)
                     /locus_tag="PLZ72_00115"
     CDS             complement(19589..20404)
                     /locus_tag="PLZ72_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNA34731.1"
                     /translation="MAPPTSRPPVVADRPLTTRSVLASTLLGTHPPRMPAPALVRAGA
                     LFGLEEGAVRTALSRMVAAGDATRDDDGWYELTGELAERQRRQEASRSATTLEWSGAW
                     TQAVVEPGGRSAADRAALRRAMGRLRMAELRDGVWLRPDNLPMDDDRTESRSVVAPFT
                     RWFRVHPDDDADLATRLWDLPSWGEHAVDLRREMARLLERLDAGDVDALAPGFVLSAA
                     VLRHFNADPLLPRELLDRRWPGDALRADYKRYDRAYRDLLAGWLRSGERGGPQ"
     gene            20476..21462
                     /locus_tag="PLZ72_00120"
     CDS             20476..21462
                     /locus_tag="PLZ72_00120"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HNA34732.1"
                     /translation="MTATVRAPLTMLPPSEALTDVLPTERLGPTGRPVGELRAELYRV
                     PNVANVGHVAGVWIQSVGVLALAAWWGNPIGWIAAFLLMGRAMARFAILAHEASHRLL
                     FTDRRINDWVGAWLLAYPAFVPFDLYRRSHMAHHREEFGPTEPDLMLYTGYPITSASW
                     RRKLTRDADGNSGWKNLRSLLLAVRSPTARPVALKILGWQVVLFVGLWVGLGHWWVYP
                     VFWLLPWMTVWRVFNRLRAVAEHGGMEASPDRRLTTHHVRQGYWARFWIVPFNTGWHL
                     AHHVDIGVPFSRLPALQRELEAAGYITPALEWPSYRALWRHARSRPDASPSD"
     gene            complement(21499..22797)
                     /locus_tag="PLZ72_00125"
     CDS             complement(21499..22797)
                     /locus_tag="PLZ72_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA34733.1"
                     /translation="MGSEPIVDPSTVTPRSHLAPVWFQVTDLQVASGEGCWVTTVDGD
                     RYLDVTSGIAVTSTGHCHPTVVAAIAEQAQRFVHAQVNCYRHDLLEPLAARLAELAPA
                     DIDTFFFANSGAEATEGAVKLAKQATGRPNVIVFQGSFHGRTHLAMAMTTSKTGYRAG
                     HTPLPSGVFPSTFPHTVGTGLTENDAVDRALADLRFLLASQTAPAETAAVILEPVLGE
                     GGYHPAPDRFLRGIREICDEHGILFIADEVQCGFGRTGHMFYVERSGVRPDILVMAKG
                     IASGFPIAAIGASAELMQRWPVGSHGGTYGGNPIGCAAALATIEVLTADGFLDAVDAR
                     GEQLRAGLSTLADAHPVIADVRGPGLMVAIEFRDPVTGEPDAARTAAVIAHCREQSRV
                     LLMNAGTWGNVIRFMPPLVISEDEVALVLDAVGAAMAATA"
     gene            22890..23861
                     /gene="pheA"
                     /locus_tag="PLZ72_00130"
     CDS             22890..23861
                     /gene="pheA"
                     /locus_tag="PLZ72_00130"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNA34734.1"
                     /translation="MTSSPSPAGSVDARRVAFLGPVGTFTEQALHREAGLAAGELVPM
                     ATMADVLFAVNEGDVDAGVVPIENAIEGTVNATIDTLAFDVDLVIQRELVEPVSMHLL
                     VPPGSPLDGVRVIHSIPVATAQCRAWLRANLPDAEVRPANSTAEAAAICARTADPAVA
                     AIANARAAEVYGLERAASDIESHPENRTRFVVVARSGIPAPTGHDKTSIVVYQRADRP
                     GSLLAILQEFAARSINLTLLHSRPTKTSLGDYCFLLELAGHVADEVVADCLRTLRATQ
                     ADVKFLGSYPAAGERGGEVRDQADAAQQAAQRWMADLRSQIRTGSTD"
     gene            complement(23839..25194)
                     /locus_tag="PLZ72_00135"
     CDS             complement(23839..25194)
                     /locus_tag="PLZ72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNA34735.1"
                     /translation="MTDRPVLDLADVGGRDMSVVGPKAARLGELIGAGLPVPDGVVLS
                     VDVHRTALAVAGVAEQVAAGVAELIERVGGAGDLDQRVAALREVAGRAEMSVGHRSAL
                     LAAYSSLVSGDADAGGIGPAVAVRSSAIGEDTEDASFAGMYDSVTDVVGADAVLAAVG
                     VCWSSSVSTRSIAYRAGMARQPSGASDPAMAVIVQRMAPSQRSGVMFTVDPRAAVDRL
                     VIEAVGAGGSVVSGGSDPERFEVDRTSGIIVDATRPSGDGDPSATSAPVLSHAEVAAL
                     NELGRSIEDHFGTPQDVEWTIGSDGSIWIVQSRPITGRPATAPGSATPGSGGSIPMSG
                     GAPTRDDPDAVLTGTGASAGTARGPARVLRSPGDAPDLLDGEVLVAPATSPEWLPALM
                     RAAAVVTDTGGMASHAAIVARERRIPCVVGVAGATERLVDGEVVTVDGSAGTVSRSTR
                     S"
     gene            complement(25191..25598)
                     /locus_tag="PLZ72_00140"
     CDS             complement(25191..25598)
                     /locus_tag="PLZ72_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNA34736.1"
                     /translation="MGSELPYTEADVDAALAAVRRICLDLPEATEKLSHSSPTFFVRR
                     TFVMFHDDHHGDGRLAIWCAAPDGAQALMVESEPERFFVPPYVGHRGWLGVRLDVDVD
                     WDEVAAIVEDAYRTVAPRKLVAMLDAPSAEPPR"
     gene            25645..25731
                     /locus_tag="PLZ72_00145"
     tRNA            25645..25731
                     /locus_tag="PLZ72_00145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25679..25681,aa:Ser,seq:tga)
     gene            complement(25757..26662)
                     /locus_tag="PLZ72_00150"
     CDS             complement(25757..26662)
                     /locus_tag="PLZ72_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNA34737.1"
                     /translation="MSRPRAIDVCRVSDGSEFHLADHPADWSGSDLLPSGLELRTKKQ
                     AVAELEVNRARLVAAQEKLWANDTYGMLVVFQAMDAAGKDGTIKHVLSGMNPQGVVVS
                     SFKVPSVEELDHNYLWRYARRLPERGQVGIFNRSYYEEVLVARVHPEVIANQRLPARA
                     RGPHLWQHRYEDMVAFERHLVRNGIVVVKFFLHLSREEQRLRFLDRIDTPEKNWKFSA
                     GDVRERAYWDDYQVAFEEAIRATATEDAPWYVIPADRKWRMRTLVSDVLVDVIDSLPI
                     STPTISDEQRAALAVERERLVAEGG"
     gene            26812..28221
                     /locus_tag="PLZ72_00155"
     CDS             26812..28221
                     /locus_tag="PLZ72_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNA34738.1"
                     /translation="MSTELLHRIQFGFTISFHFIFPPMSLGLGLLLIVFGVQAVRTDD
                     PKWRQLAMFWTKIYGLIFSMGVATGLVQEFQFGTNWSEYSRYVGNIFGSLLAAEGIFA
                     FMLEGGFLGLMLAGGRRLGNKLWLFATTMVVLGATFSATWIIMANSWMQTPQGYELRN
                     EGRGTQAFMSSFREVVFTPSFGPRFWHTIVAAWMVGCSLVMSVAAYYLLRRRHEDLAK
                     TMMRVALPLFTVLAVLQVTVFGANQATEVATHQPEKLAAIEGVYQTGDCVPMTIFGRT
                     DPSTQTTQGLQIPCLLSILVGKSPSTTVQGLDDFPTDDWANASVVFQTYHLMINLGMA
                     FVAIGGLGCLLWAWKRRLWTQRWLLWIMVGSIGLTQLATLSGWWTAELGRQPWIVWQL
                     LRTEDAFSPNVTTAQVAASLAMFAVLYAIVFGVFLVLLNRLIQAGPPDPPTEEEVQAL
                     PDTWGAAFQHRSRVSDGSR"
     gene            28223..29242
                     /gene="cydB"
                     /locus_tag="PLZ72_00160"
     CDS             28223..29242
                     /gene="cydB"
                     /locus_tag="PLZ72_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNA34739.1"
                     /translation="MNLETLWFVLYVGIIAGYVILDGFDLGVGILSPFVARTDRDHRM
                     LLNAIGPVWDGNEVWLVLGGGALFAAFPVVYASLFSGFYLAMMLVLLVLILRTIAIEF
                     RSHRESPRWRATWDWFFFASSLGITLLLGVALGNVIRGIPLDSSGQVTLKLVDLLNPY
                     SLAVGVTAVAMLGLHGGIFLTLKVDGELRGRIERLVPRLMVAFFVLMTVLVVWTLLLR
                     TEVTATYRDRVWIAAFPVLALVSTVVAWRLIRAGRFLPAFGASALTIALLMATVAAGM
                     YPVMLPSSIDSSYDLTVHNSASAPETLTVMTVIAVIGIPFVLLYTAGVYYFFRGRVVL
                     DDHGY"
     gene            29352..29443
                     /locus_tag="PLZ72_00165"
     tRNA            29352..29443
                     /locus_tag="PLZ72_00165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29388..29390,aa:Ser,seq:gct)
     gene            29792..30193
                     /locus_tag="PLZ72_00170"
     CDS             29792..30193
                     /locus_tag="PLZ72_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNA34740.1"
                     /translation="MNRKSLTRALALLLVVGGFVLASCTPEQQAAFQAHLDWQKANDR
                     FAGAISDAGLARLRACESGGNYSAVSRNGLYRGAYQFRRGTWNSVAGKFYPHLRGVDP
                     ASAAPFDQDRMTRALWATGGPRNWPVCSRRV"
     gene            30402..30475
                     /locus_tag="PLZ72_00175"
     tRNA            30402..30475
                     /locus_tag="PLZ72_00175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30436..30438,aa:Arg,seq:acg)
     gene            complement(30755..31546)
                     /locus_tag="PLZ72_00180"
     CDS             complement(30755..31546)
                     /locus_tag="PLZ72_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA34741.1"
                     /translation="MTTPTFDTLRIEHDTDSRVATLVLNRPGKLNALSRQLLGELARA
                     CRWLDDRGDIAVVVVRGEGRAFSAGFDLGDVADGDPTISVRDTADLGRVAAEALTDVR
                     QLTIAALHGHCVGGGLVLAASCDIRVAADDTRFSIPEVDLGIPLAWGGIPRLARELGP
                     AITKELVLTCRPFDAAEAHALRFVNRVVPAGELDVVVADLAAELGAKPGFALRTTKQQ
                     VNAVMEEIAGTRRNASDADTLAAAVADPESRQASLRYLEARRPSG"
     gene            complement(31543..31839)
                     /locus_tag="PLZ72_00185"
     CDS             complement(31543..31839)
                     /locus_tag="PLZ72_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34742.1"
                     /translation="MNISVCGLTRLVVPAFFVGFTAAVWTGRDVVGWVVAAAVAAGLY
                     GWGRFRGTSVSCPVPTAAGRSTPSVAESDADVRHDATGTATGVDTVTDREGSLP"
     gene            complement(31836..32516)
                     /locus_tag="PLZ72_00190"
     CDS             complement(31836..32516)
                     /locus_tag="PLZ72_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA34743.1"
                     /translation="MHHDHDPHRPDHDDHGPASFDERAATWDDDAKVARSGRVADAVL
                     RATGVDRSARVFEYGAGTALVTEALGDRVGPSVLADTSAGMREVMATKVADGRLPRSR
                     VVDWDLGADSIPSIADRFDLIVTVLTLHHVDDVDRVLGRFADLLAPDGRVAVVDLDAE
                     DGSFHGDGFTGHHGFERDDLVRRLVAAGLDEVTVSDCGVLERDEGTFPMFLAVARSSQ
                     ARTGGTGS"
     gene            32629..33597
                     /locus_tag="PLZ72_00195"
     CDS             32629..33597
                     /locus_tag="PLZ72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HNA34744.1"
                     /translation="MKAAVYSETGPPSVLRYEDVPDPSVGLGTVLVRNQAISIEGGDV
                     LHRSGGELGRVPHVVGYQSAGTVEAVGDGVTSMTVGDRVVTVGVDGSHAELRVVPEGF
                     TWRIPDALSTDDAATVPVPFGTAHDALFEFGRLTDGETVLVQAGAGGVGVAAIQLAKR
                     AGARVLTTASSPAKLARLAELGADAGIDYSEQDTVTEVLRLTDGRGVDLVVDNVGGSA
                     MTDNVRCLAYRGRCVSVGEVGREGATTIDVGMLRPKNLTVVGYFMGMEMLMSPRCHDV
                     VARLLDDLAVGELHAVIDRTFPLSEAADAHAYIESRAAVGRVLLRP"
     gene            33699..34313
                     /locus_tag="PLZ72_00200"
     CDS             33699..34313
                     /locus_tag="PLZ72_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34745.1"
                     /translation="MTDAVGVDPGSTPATEHGPPPMDVRIIPPMGLPFVAVAVVFVLY
                     AIGSNSIWALMLAHVAGGGMWTAVDLYVGLILGPILGRLSPPARAEFSARFMPKMVLL
                     MPTLVMMTLATGFQIALNYGNLYPSSINRPWLVASMVVVGIMAVVALGILEPANISVL
                     FEMKKEQPDVDVIEHLMKRFVYTAGVTGLMQVATLLIMTRVATQ"
     gene            34310..34744
                     /locus_tag="PLZ72_00205"
     CDS             34310..34744
                     /locus_tag="PLZ72_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34746.1"
                     /translation="MSSVGPATVRPFERSLPPVTGLGMAALACAVVSGVVVASQVGMD
                     DPPLVVPTVFVAAALLLEIAAVVVLVRIRPFAWDRFRLVFSVGLAAYVLQSAIIAWAF
                     VINDVPAGPMTILSLGLVVFATIVPVMLAFTVARYQAVDDPR"
     gene            34914..36035
                     /locus_tag="PLZ72_00210"
     CDS             34914..36035
                     /locus_tag="PLZ72_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA34747.1"
                     /translation="MADHEEQAGSGQGAVSRRAVVGATVGGIGAATMVGVAAAAAWPD
                     SGGGSGGSAAPAEGGGSGGTTAASSGAQIDFAAKPADDWVARDPVLPPAPAERVHNIT
                     MTAYDQDVAEVAPGVKQQIWTYDKVMPGPFYRGKVGDQFNFTLKNEGTLGHSVDFHAS
                     KVAWNVRMATINPGESIEYPFEAKHAGIYMYHCGTPPALHHIGAGMYGAIIIDPPDLA
                     PVDHEFAFVQSEIYTGPQGEVGSLEKMQKSAWDAIVFNGYVNGYSARPIRVEPGQRIR
                     AWVLDAGPSENSSWHVIGTVFDTVFKEGNYLLRPGDGQRGGSQALDLQPAQGGFVEFS
                     FDEVGLYPFVTHKFANVGIGALGIFQAGDVQPAETASGH"
     gene            36046..36564
                     /locus_tag="PLZ72_00215"
     CDS             36046..36564
                     /locus_tag="PLZ72_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNA34748.1"
                     /translation="MAVDQESSDTTDSPAGPSGAVHSGPVPVRKGTTFETLAVCGFIF
                     GLFAIVAAIFAVGLAARAASDASGSGGGGSAGGGTAGGGGAAVTEVSLGEFFIKPQDA
                     TAAADSVLKVKNDGTVDHDLAVEGGPATEMLKPGDEGELDLAGVAAGTYTWICQVPGH
                     AEAGMKGTVTVQ"
     gene            36675..37070
                     /locus_tag="PLZ72_00220"
     CDS             36675..37070
                     /locus_tag="PLZ72_00220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA34749.1"
                     /translation="MAFSASPFPLQEHLGMAIESPSAGSGRAVLDIGPDLLNPNGVVH
                     GAVLFAMVDTAMGAATMSVLEDGQACASIEVHLRFLRAAAAGSLTADVSVIRAGRRIV
                     QLEGRVTGADDELIATASGSFAVITGPAT"
     gene            37093..37764
                     /locus_tag="PLZ72_00225"
     CDS             37093..37764
                     /locus_tag="PLZ72_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA34750.1"
                     /translation="MSQRRSSSEDSATRDRLLAATTNLIARDGIAAATSRRITDEAGV
                     NLAAITYWFGSKENLVDAALRGQVAALVEPALAILERDGDPVTRMAAAVRYLLSTFAE
                     RQGQSAAYLTALLEVTRHSDPADPTRAVVARVRERLAAVNAELVASGAVPAWVDPDAM
                     AALIIAAGHGIALQSTLDPDGPTVESQAAQLAGLLIAARPHDGPTEASRPRGEDSGRT
                     TNRSE"
     gene            37736..38371
                     /locus_tag="PLZ72_00230"
     CDS             37736..38371
                     /locus_tag="PLZ72_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34751.1"
                     /translation="MDEPRTGRSDPTDADRTEHRRDPVRDRPSSGSWVDVLGVLLVLA
                     GVALAVIRWFGADPLERNAESLLASVTLGAVIAAPGVLCLIATRTDRNALLLPAAIVL
                     ALLSTLSPATLPLFIGALALAWRWARVGPGHGRLRGALATVVVVVGLLGAVVLLLVRT
                     TERTFYGNGVVHSTDGWQPWSTSLLVLGLVALTVAVACSLVPAGSSPTRTP"
     gene            complement(38464..39222)
                     /locus_tag="PLZ72_00235"
     CDS             complement(38464..39222)
                     /locus_tag="PLZ72_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34752.1"
                     /translation="MTEETSHNRELWNGVNTAFTDRDGERRWQEPDLRWGLFARPEAD
                     LALLGDVAGLDVVELGCGTAALSAWLARHGARPVGVDLSPAQLQTARRCQRRWGPEFP
                     LVEADGEQVPLRSAVADLVVSEHGVGAWCEPERWVPEAARLLRPGGRLVFMTNSPLSA
                     LCVPDDPGPAGDRLLRGVDDVRTVRWDGGGVEHHPGHGEWVGVLGRSGFTVDALHELR
                     PPVGAEDPVWYEIVSADWAGRWPAEDVWVARRTG"
     gene            39334..40182
                     /locus_tag="PLZ72_00240"
     CDS             39334..40182
                     /locus_tag="PLZ72_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34753.1"
                     /translation="MGHTTTTPVDPDALPGHALQADRMPGHWLLARLGKRVLRPGGAE
                     MTRNLLGGLAISTADDVVEVAPGLGATTRLVMAARPASYTGVDRDPDAVGLIGDMLEG
                     PNRRVIHTSAQDTGLPDASADVVFGEAYLTMQPDSLKHKVIAELARVLRPGGRFALHE
                     VAFAPDDIADPDRERATSDLTSTIKVNVTPMTVAGWDQLLAEHGLVVRSRFKAPLHLL
                     EPRRLVADEGYLGAARFVTNVVRDSEARVRVRAMRTAMRANASHLQAYGIVAERVDPA
                     GSGAGA"
     gene            40179..40964
                     /locus_tag="PLZ72_00245"
     CDS             40179..40964
                     /locus_tag="PLZ72_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34754.1"
                     /translation="MSGGRRAAVGIGAVVVVVAAAVAFLLWPRGTTEISEKDAVEDFR
                     EKKSTDTTATDGQGTGTPTPGVYTYSAEGQERVKLGPLPAQDRPLPATVTAVVTDTGP
                     GCFQWTVNLFAEHTEDTTWCTNPSLRMEEHAKHQTIGAISPTATMTCDPNQLPAAGGT
                     PTELPCTLKLSGGPMSVTATFTGTATAKAGEAVDVGGTKVETTPIEIRYPVSGDINGT
                     WVETTWWSRDHLPIRVTRALELAGTASFTENTTLQLTSLEPTS"
     gene            complement(40972..41472)
                     /gene="thpR"
                     /locus_tag="PLZ72_00250"
     CDS             complement(40972..41472)
                     /gene="thpR"
                     /locus_tag="PLZ72_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNA34755.1"
                     /translation="MPRLFLAVWPPEDTVSELMALRRKDQRGVRFVAPERWHVTLRFF
                     GEAHPDDVEEAMADVDLPPAVATLGPAVDVLGERVLAVPVNGLEALAGVVVAATRGVG
                     DPPPRRAFHGHITIARIARDARMPPTLGTRVDGTFCVEELALVRSRRDPDGVRYDTVA
                     TWPLPA"
     gene            complement(41562..42524)
                     /locus_tag="PLZ72_00255"
     CDS             complement(41562..42524)
                     /locus_tag="PLZ72_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA34756.1"
                     /translation="MAPTRSLVIDDPSPDGLAVPRDMSAGYGDGLPVGLCLGGGGLWF
                     VAWQVTYLNELLGAGVDLSGADRVVGTSAGSIVSSVLEAGNIKRLHGELRVLARLPKL
                     LGVLAPASNFSPSQERAQEMFWSATDADPDTVRAIGHAALNADTPSAATMRRNLGAVL
                     AFRRWPSPALHLSAVDAYTGERLVLTDRTGVSVPRAAAASSAVPGLFPPQQILDRRAM
                     DGGLAGTGLHLDLLAGARRAVVVQLTDGTDPDLPSQGGMTQSPGDAVAELEALEASGT
                     KVFSRMPEKVDIERLMDPTAVPDAIAMAERQARADVEELRAFVA"
     gene            complement(42555..43037)
                     /locus_tag="PLZ72_00260"
     CDS             complement(42555..43037)
                     /locus_tag="PLZ72_00260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNA34757.1"
                     /translation="MTAPTPLRARTIETPIGSLTLAGDDRLRYLLMVDQAHAPDRTGW
                     RADPSGFDDVVEQLDAYFAGELRSFDVAVEMRGTEFQRTVWRGLCDIPYGETWSYAEL
                     AAHIGRPTAVRAVGAANGRNPVSIIVPCHRVIGADGSLTGYGGGVDRKRALLDLESGR
                     "
     gene            complement(43034..44602)
                     /locus_tag="PLZ72_00265"
     CDS             complement(43034..44602)
                     /locus_tag="PLZ72_00265"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HNA34758.1"
                     /translation="MPIDWKAVDRNAVDPSALDPERCYRVVCARDARFDGWFVGAVTS
                     TGIYCRPSCPARTPRRANMRFYPTAAAAQSAGFRACKRCRPDASPGSPEWDPRSDVVA
                     RAMRLIADGVVDRDGVEGLAGRLGYSARQVERLVRAEVGTGPLALARAQRARTARTLI
                     ETTDLAFAEVCFAAGFSSVRQFNATVREVYDLTPGELRIRARRAGPAEVDRVVLRLPV
                     RAPFAASELFGRLARQAVPGLEEAVVDPHDGDVVTFRRALTLERGAAVVGLTPRSDHV
                     AAEVRLEDLRDLTSAVARCRWLLDLDADPVAADAVLAADPALAPSVAAHAGLRAMRSV
                     DGTETAVRVLLAQQVSTAAARTHAARLVAATGVALPHRLTVGTGITHRFPTAGELAVQ
                     LRGARAEGIPLALPARRRATLLALVEALAAGWIDLGPGADRARSRSRLAEVPGIGPWT
                     VEVLAMRTLGDPDAFPVTDLAVRRGAVALGLSDRRSELVERSEAWRPWRSIATHHLWL
                     AAAGRPTCVQEASP"
     gene            44804..45874
                     /locus_tag="PLZ72_00270"
     CDS             44804..45874
                     /locus_tag="PLZ72_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA34759.1"
                     /translation="MPILPPPPVGSFVSDNAAGVSPEVMEALAAANVGTAMAYGDDDW
                     TRRAQARLREVFDAPVHSYFCWGGTGANVVGLASVLRPWQAVLTAASAHIVVDECGAP
                     ARFTGSTIVPIPTDDGRLSPDMLAPWLQWQGSEHHPQPAVVSITQSTEMGTLYSVDEI
                     GALCDAAHAAGMLVHLDGARIANALVATGSDLPTMVRDTGVDLMTLGLTKDGAMFGET
                     VVFVAAERDRAPLDAEARFVRKQAGQLVSKSRFVAAQVLALLEDDLWLANARHANLMA
                     ARLARSVEAIPGVRLADEPQVNSLFVHLPAAAIAPLQEWSFFWDWDLTVDLVRWMTSF
                     ATTDEDVQRFAAGVSAVLADHA"
     gene            45910..46890
                     /gene="map"
                     /locus_tag="PLZ72_00275"
     CDS             45910..46890
                     /gene="map"
                     /locus_tag="PLZ72_00275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNA34760.1"
                     /translation="MKAGRNAPCWCGSGAKLKRCHLHRDVLRRPAVHLGRVGALRSVP
                     AAIARPPYAFDPPTWPLPEPQILSGAALERMRHAGRVAAEVLAETVAAVGVGVTTDEL
                     DAVAHDAYVARGAYPSTLGYHGFPKSICTSVNEVLCHGIPDDRPLADGDIVNIDVTAY
                     VDGMHGDTSATVGVGTISPAAAALIDTTRDATLAGIADIVAGEPMQRIAAAITAVASA
                     RGYGVVAEYGGHGIGEVFHADPHVHHTLTSRDDSVALPGLCVTVEPMLRTGAPRFTQA
                     DDGWTEILDDGMPSAQFEHTVVVTDDGAEILTVLADGSSAVSPTGPASAA"
     gene            complement(46896..47921)
                     /locus_tag="PLZ72_00280"
     CDS             complement(46896..47921)
                     /locus_tag="PLZ72_00280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA34761.1"
                     /translation="MNPTVPLSVLELAVLTEDGTSADALAGATEVARAADRAGFQRFW
                     VAEHHNLPSVASTTPPVLMAHLAAVTDRIRVGSGGVMLPNHAPLVVAEQIAAIEALHP
                     GRVDLGIGRAPGTDPYTAMALRRSADLSAEDFPRDLLELMGLLGDPRPDAWGELVGGD
                     RPLWDRFAATPRAVSRPQILLLGSSGYSARLAGLLGLRFAFAHHFDMGGTDQAVELYR
                     RSFRPSADLTAPHLIVTANVLVADTAAEAERQALPGQLLVHGIRTGERIRLVDPDEAP
                     RHRWADVARVARTNRIVGPPERAASELAALAERTGADELMVSSVAHDTAVRVRSLELL
                     LEAFRGR"
     gene            47972..49900
                     /locus_tag="PLZ72_00285"
     CDS             47972..49900
                     /locus_tag="PLZ72_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNA34762.1"
                     /translation="MTSAPSSPIAPGPLIEDHALIGDTVGVALVDRSGSIDWWCPSRV
                     DAPACFAKLLGTADNGHWRLAPRDPVIEVRRRYEPDTLVLETEMRTASGTVAIIDFMV
                     PEQEHPTIHRWVEGRSGTVSMGMELVVRFDYGAVVPWVRSTGDGLDMVGGNEALRFHS
                     PVPLHGRDLRTYAEFDVSVGEQRSFSLAWHPSTDPAPLPLSTFAALHRTRTWWRTWVS
                     HCTYSGPWRHDVVRSLITLKALTNSRTGAVAAAATTSLPEWIGGVRNWDYRYSWLRDA
                     TFTLQAFLIAGYVDEARAWSRWLRRAVAGRPGDFQILYGVGGERRLPELELDWLAGYE
                     GSAPVRVGNAASGQFQLDVFGEVMDAAATARRAGVLPLGRDGLDVARALMTHLEGVWD
                     QPDEGIWEVRGPRRHFTHSKVMAWVAFDRAVRLASDAEVGAFAVDGHRRPPDPGRVAR
                     WAELRDRVHDQVCTNGWNDDIGAFTQYYGSTELDASLLMVLPVGFLTPDDPRAASHVD
                     AIGRELSVDGFVRRYRTEPDDAHSVDGLPPGEGAFLLTTFWWADNLAMLGRVDESIEV
                     FERLRSLRNDVGLLAEEYDPTSGRMLGNFPQAFSHVGLVNTAANIAAAQGHPETAVAL
                     TRFTRCPDPSPSGVDRTS"
     gene            49897..51318
                     /locus_tag="PLZ72_00290"
     CDS             49897..51318
                     /locus_tag="PLZ72_00290"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNA34763.1"
                     /translation="MSTADPTPLETPGFRIEHDSLGPVPVPIEARWQAQTQRAIDNFP
                     ISGGRVAPGLIRALVEIKRAAALVNVEEGHLDRVAAEAIVSAADEVLSGLHDDQFPID
                     VFQTGSGTSTNMNVNEVLATLATVRAGTPVHPNDHVNAGQSSNDTFPSAIRIAAATRI
                     VGHLIPSLQHLARSLRRAEDRFADVVKAGRTHLMDAVPVTLGQEFGGYATAVESGVRR
                     LRSVLVPLGELPLGGTAVGTGLNADPGFAAAVIARVAERLDLPLTEAPDHFEAQGAQD
                     VLAEASGACRVVAIGLNKLAADLRWMSSGPAAGLGEIHLPDLQPGSSIMPGKVNPVLP
                     EATQQVVAQVFGNDAAVAFAASAGNFELNVMLPVMARNLLESIELLANVSVLLADRCI
                     DGITADVDRARRYAESSSAIVTALVPLIGYEAAAQVAKAALASGRTIREEVVARGLVS
                     DPDELDRALDVLAMTRGGRLTDR"
     gene            complement(51399..53330)
                     /locus_tag="PLZ72_00295"
     CDS             complement(51399..53330)
                     /locus_tag="PLZ72_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34764.1"
                     /translation="MRLVDLQVGTDNGVHLELHPRLNVVIASAALQERVAALLGRAYV
                     LAGTEVTGTVDGGDYLTPLDPTAVVALDLIGEGLDRIGPADLPAPDPTARQRARAVLD
                     ARIDAANAALGELVEERDRIARLQAATEAAVAAGTEEREECVARREVLDVSAGALDAR
                     RPALATERDSAGHAADVAADRLEELCGVRAALVTELGPGEDGADIRMGDDTGELVALV
                     ERAGALGGLSAQQMDEVRTWLRDVARSQVPVRADAQALLAEVDAVERAWQLAARTGVE
                     GDPEVVALASERADLDTNHQLLRGLAASGMLGDTAKSQIDASHIALLQASKAQEADAA
                     AAEQVVLARYGFDSYLEYTIATSTRSVGQAVEAKLADLDEHIESLDRELAAARSRVAE
                     RIEQLAAEREPAQQKVTALLGFRPEGSSIDALSRIPEVPHSVTRLTLAVDDAIETARD
                     EVARYRGTIADLDDEEVSLGERARELVGQREQIDARIADLDVALGDAVPEAASLAVRL
                     ADAEAGVANATRDLSGITDERDRLDAVPADRYGPDDVVPVVEAVLRRLDPWATNPPPV
                     VLCDTFGPLPSAQAIAVLEAVTARADRSQILYLTSSSEVRDWAKRLDPSVGRLINLAH
                     GRWSPRRLGRKVLGRRQSG"
     gene            complement(53397..54224)
                     /locus_tag="PLZ72_00300"
     CDS             complement(53397..54224)
                     /locus_tag="PLZ72_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA34765.1"
                     /translation="MDLQSILDLESHGRDVYVGIGPRYPWGGLYGGQIVAQALMAAAH
                     TVSDEMPVHSLRAYFIRRGDHDEPVRYEVDRIRDGRSFTTRRVVARQAVGAILNLEAS
                     FQRDEGSADVETLAPPPELPTPDQLEPSSWSAAFERAFVPGDRVSPAAERDGRGRATA
                     WLRTTEAIGGDRFRQAAALAYMSDDLPTDAVVQAHPVRSEPDETLYKALFTASLDHTI
                     WFHRPLAADRWHLHDFTCHTFVGGRGLSLGQIFALDGTHVATVAQEVLLRDTRDRAS"
     gene            54453..55265
                     /locus_tag="PLZ72_00305"
     CDS             54453..55265
                     /locus_tag="PLZ72_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA34766.1"
                     /translation="MTEPNASTAIPPVDEKLLEQAVAWTRAAGELTLGWFNHPELGVE
                     HKHDGSPVTAADRAAERLLREEIAAALPDDTVRGEEEGDRVGTSGRTWVIDPIDGTLA
                     FSRGVGTYSNLLYMEDEHGPAIGVINLPALGETVWAGRGRGCFLDGARCSVSDRAELS
                     GSYLSVTGFHEWTPDMFDRVTGSGLQLRTWGDAYGYALVASGRVEAMFDPALAWWDLA
                     AMLVVIPEAGGTLTSWSGEDPAVENIDHPEYRWSAIASNGAFHDRLVEVLAR"
     gene            complement(55269..56225)
                     /locus_tag="PLZ72_00310"
     CDS             complement(55269..56225)
                     /locus_tag="PLZ72_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNA34767.1"
                     /translation="MGAALTEDRPTRDTERTLVPNRRSASSQDASRLVEDHLDLVNHV
                     VFQVAVHFPRHVDRDELARAGALGLVEAARRYDEARGVPFNRFAAQRIRGAIIDAVRS
                     ADWAPRSVRTLARRLDATEQRLAAQLGRIPSIGETAEALGMSRDELDRLRDRIFRSVV
                     LAFEHLVGDTEDEELTLIDVLADPDEREPAEELEQRELHAYLRDAIALLPERHRVIVL
                     GYFIEDRTSEELARFLGVTESRVSQMRTEALGMLKMGIEAQYVPVEDREAPDGLVARR
                     RARYVDAIGAATAPTDRITERDHPESDTAFVDLVQSLVGAGG"
     gene            complement(56497..57540)
                     /locus_tag="PLZ72_00315"
     CDS             complement(56497..57540)
                     /locus_tag="PLZ72_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA34768.1"
                     /translation="MSGPDASEPVRPRVAVVGAGIVGARAARELISPGADGRPGASHV
                     SISTRREERRDQLRATFGASVPVLWQTDRVDGSDSRGPADVVLVAREPREQLEVVSAA
                     LDAGSHVVATTDDPEEVEAILRLHPRAVAAGRSVIVGVGLSPGLTCLLARHAADLLDT
                     VDEIHVARDGAAGPACARQRLRALRGAAQEWRDGTWVRRAGFSGRELLWFPDPVSARD
                     CYRADLAEPLLLLDAFPDVTRLSARLAASPRDRVLAPLPVLVGPPVEGGPGAARVELR
                     GTRDGRREMVVYGVFDRPGVAAAALAAVLALRAAAGDVAPGSHGVAASVDPRPVLAEL
                     ARRGVRAAAFDPA"
     gene            complement(57537..57674)
                     /locus_tag="PLZ72_00320"
     CDS             complement(57537..57674)
                     /locus_tag="PLZ72_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34769.1"
                     /translation="MRGLRRAAAAVAIGGLVGLVLRFRGSTEPPLQGGGWRELTGDDL
                     R"
     gene            complement(57740..57816)
                     /locus_tag="PLZ72_00325"
     tRNA            complement(57740..57816)
                     /locus_tag="PLZ72_00325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57780..57782),aa:Ile,seq:gat)
     gene            complement(57941..58471)
                     /locus_tag="PLZ72_00330"
     CDS             complement(57941..58471)
                     /locus_tag="PLZ72_00330"
                     /inference="COORDINATES: protein motif:HMM:NF023511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HNA34770.1"
                     /translation="MSDTRPADPVGTTRAVPSPPSGPPARRRAVEVESDPTPNRVLGY
                     RRQRFEAKKVRRLVRHVDPWSMLKLAVLVCLCMWLISMIAVVILWTVARSSGTVTSLE
                     KFVNSSLQLQDWKLDGEFLFRQFGLISLLFHLGLAAAIVVATLVFNLISDIIGGVWVS
                     VIEEETARPVQESGPS"
     gene            complement(58523..58783)
                     /locus_tag="PLZ72_00335"
     CDS             complement(58523..58783)
                     /locus_tag="PLZ72_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34771.1"
                     /translation="MAVAVVCAVGVLRVGDAAARRARADAAADLSALAHVTGDLDAAT
                     AVARANGAVVTGHRRTGAARVVTIDLDGVVATAAARPAGSAR"
     gene            complement(58818..61418)
                     /gene="gyrA"
                     /locus_tag="PLZ72_00340"
     CDS             complement(58818..61418)
                     /gene="gyrA"
                     /locus_tag="PLZ72_00340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNA34772.1"
                     /translation="MSDDTPQDPTPPTDGDPAGSQPGEDAVAVTFGSIEPIEIQQEME
                     QSFLDYAMSVIVSRALPDARDGLKPVHRRILWAMHEAGLRPDRNHKKCATVVGDVLGK
                     YHPHGDQSVYDALVRMGQDFSLRHPLIDPHGNFGSPSDPPAAYRYTECRLTELAMHML
                     ADIDEDTVDFVPNFDGSTEEPTVLPSRFPNLLVNGSQGIAVGMATNIPPHNLGEVIDA
                     TVHLLENPEATCDDLMEFVKGPDFPTGAQIMGRAGIVSAARTGRGSIKMRARAEIDEG
                     PRGERIVVTEIPYQTSVESIEEKIAELANARVIEGVRELRNESSKGRPRLVIELKKDV
                     AANVLLNNLYKHTPLQTTFATNFVALVDGVPRTLDLRAAIAHYIEHQVDVITRRSRFR
                     LAKAERRAHIVEGFLRALDLIDEIIAAIRASADRASAIAALMGDAFSFSEIQATEIVD
                     MRLSTLTRLGRERLEEEMAQLRATIAELQAILSDDGRLRSVIREELVAIRDKFATERR
                     TSLEHDDGDLEIEDLIDDEEVVVVMTARGYIKTVAADVFRTQARGGRGVAGAKLKDED
                     YITDLIHTSAHAFLLFFSNRGKVYRLKAHRIPQRDRTAQGLAIVNLLRLQPDEKIQTI
                     IDTRDYESHRYLFFVTANGVVKKTLFNAYDSSRQDGLIAINLRDGDELVRVLPTSDDD
                     QILVVSEGGQGIRFSETDVRPMGRTASGVRGMKLRPGDRVVGAGAIAEGVRLLTITDA
                     GYGKRTDVDQFNTQGRGGQGVRSHKLHADRGRVITGLLVADDDQIFLINDSGTVIRTA
                     VDTISVQGRDATGVRVMNLDDDTRVAAVARVLGGEEDDDGSDDGGSDDATGTEAGTEA
                     GTDSGGVDPD"
     gene            complement(61434..63389)
                     /gene="gyrB"
                     /locus_tag="PLZ72_00345"
     CDS             complement(61434..63389)
                     /gene="gyrB"
                     /locus_tag="PLZ72_00345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNA34773.1"
                     /translation="MSAEPTTYDAESIQVLEGLEAVRKRPGMYIGSTGPAGLHHMVYE
                     VVDNSVDEAMAGFATRIDVRLLADGGCEVTDDGRGIPVGTSVQFPDMSAAEVVLTVLH
                     AGGKFGGGGYKVSGGLHGVGISVVNALSTRVEVEIDKNGRHHAMAFADGGDLVDRLHE
                     TGDSPTGAGGEPRTGTTVRFWPDPSIFDDITFRAATLTERFQMMAFLNAGLRITFRDE
                     RPDHSGEPVEFHYEGGIRDFVRHVNAAKEALFTDVGYFRQDESDDDGSMEVEVAFQWN
                     TGYNSDGLHSFANGISTIEGGMHEEGFKKSLTNTVNRYAREHGFLKDKESNLQGEDIR
                     EGLTAIISVKLTDPQFEGQTKAKLGNVPMRSLVERATNDKLSEWLEEHPSEAKRILQK
                     SVQAARAREAARNAREATRRKSALDGAGLPGKLADCSSKDPRESELYIVEGNSAGGSA
                     KDARNPATMAILPIRGKILNVERARLDKMLKNNEVQALISAIGGGVGDEFDYDRIRYH
                     KVILMADADVDGSHIRTLLLTFFFRQMRPLIEGGHIYIAQPPLYSTEVGRNKIYLKDD
                     AAKAAFLADHPNHRNEFQRLKGLGEMDADELWATTMDPAQRTLLQVSVEHAVIADEVF
                     SILMGDDVESRKHFIQTNANDVRFLDI"
     gene            complement(63605..63931)
                     /locus_tag="PLZ72_00350"
     CDS             complement(63605..63931)
                     /locus_tag="PLZ72_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNA34774.1"
                     /translation="MAIEPLPRDRDTLHPLAGSLERLQRSLGLARPDTLALLTESWPQ
                     LVGSRLAAACRLHSLRDGCLTVAVDDPAVAEHLRWQSSDLAAAANELCGFEVVERVEI
                     RVDPRR"
     gene            complement(63942..65039)
                     /gene="recF"
                     /locus_tag="PLZ72_00355"
     CDS             complement(63942..65039)
                     /gene="recF"
                     /locus_tag="PLZ72_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNA34775.1"
                     /translation="MQIQRLWLNGFRSYVDADVELPGGLTAVLGPNGQGKSNLLEAMS
                     YLATLRSFRGAPAEAMVRVGAERAVVRGQVVTDGREHLVEAEIARTGRNRVLVDKQRL
                     TRQRDLTEVLRVTVFAPDDLDLVKGGPALRRGFLDDLLVSVHPRHDQVRAEWERALKQ
                     RNALLKQMHGRPDESALITLDVWDQKLSTAGDALAGLRHDLVQCIAPVVSRAYRDVAA
                     EELDVSLRYLAPWSTGTGDRPGGLAAALAASRSDELRRGVTLVGPHRDDLELTLRSLP
                     ARTHASQGEQRSLALALRLASHRVLLETHDSPPVLLLDDVFSELDPERSAALLRSLPE
                     GQTVLSSAAGLPDGVDSELILDVADGAVRPR"
     gene            complement(65071..66162)
                     /gene="dnaN"
                     /locus_tag="PLZ72_00360"
     CDS             complement(65071..66162)
                     /gene="dnaN"
                     /locus_tag="PLZ72_00360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNA34776.1"
                     /translation="MKFRCERDVLVDALGTAGRAATSRAALPVLSGLRLQLSGDQLRI
                     TGSDLDLTITRSLSVAGAEDGVAVLPAKLAVDIVRALEPGGVEVDTSGDRPSIRAGRS
                     EFFLNAIPADEYPNPREPEGESVTLQSGHLADGLRQVVGAASSDESRPILTGVLLAAE
                     DGGLRLVSTDSYRLAYRDLPGTSLLEQGQQVLVPSRSLQELTRALGEDDDVTLVLSDQ
                     MVSFTVGDLQLSTRLIKGDFPDYRKLIPTDMPNALTVERQALADAIRRVRLMARENSP
                     IRLSMGPDGLELRAITQDVGEAVEQLDAKYEGTELTVAFNPEYLMDGLEVTPGDEVVL
                     QTRDAQRPAVLRTTDESDFLYLLMPVRVS"
     gene            complement(66419..67804)
                     /gene="dnaA"
                     /locus_tag="PLZ72_00365"
     CDS             complement(66419..67804)
                     /gene="dnaA"
                     /locus_tag="PLZ72_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNA34777.1"
                     /translation="MTAEGTTVWQRTAALLVEQVSDTVWSSTFQDIHADELHDGVLTL
                     VVPSQLVRQRVEQRYFPMIEAAVADAGFPEVRLILEVEIDDVRPDPDDGLTVDLTDPD
                     LAAVRPLSATPAEEPAPSPIGPPRYTFDSFVIGASNRFAHAAALSVAETPGRRYNPLF
                     VYGGAGLGKTHLLQAIAQYVRDNYPTYRIRYISTETLLNEFVDAIRKNQQPDFKRRYR
                     EIDVLLVDDIQFMEGKEQLQEEFFHTFNTLYESQRQIVLSSDRPPDAIATLEDRLRSR
                     FKMGLITDIQPPAFETRLAILQKKNEQAATSVPADVLSYIAEHITYNIRELEGALIRV
                     GAYAALNQTELTVDLAAEILADTIKSTKPRQITPELILTQTAEYFKMDVEQLQSRSRT
                     RNLVHARQIAMYVCRELTDLSYPQIGREFGGRDHTTVIHAYEKVAALMQEKHETYEDV
                     TALIQSLLAER"
     gene            68241..68375
                     /gene="rpmH"
                     /locus_tag="PLZ72_00370"
     CDS             68241..68375
                     /gene="rpmH"
                     /locus_tag="PLZ72_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNA34778.1"
                     /translation="MKRTYQPNTRRRARKHGFRHRMSTRAGRAIVKSRRRRGRARLSA
                     "
     gene            69176..70429
                     /locus_tag="PLZ72_00375"
     CDS             69176..70429
                     /locus_tag="PLZ72_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNA34779.1"
                     /translation="MFEQFFQALGSVLNFFYTLIPNYGLSIMLLTVLVMALITPLNVK
                     STRSMLQMQRLQPEMKRLQAKYKDDREQLNAELMKFYRENKINPLGGCLPLLAQMPVF
                     FIMYSLLRGLTVREGGLGSGIGHIAGQVQQGVELTPWVFTDQYFRPEHLNHSTELYQS
                     LSHTNTMNFFGMDLAISAAEAVKIGLLVAIPYLLLLVVILITGVYQNRQLQARNTNAT
                     VNPQQQMIMKVMPFFLPVFSFGFPSGLALYWATQNLCRIGTNSYITRSVYRKEHAKGP
                     IEATASEKTSTSAKKGSSGSKGGSATGGTDRGKSRAGGQKSAPKSKATVDPSGSAKSA
                     DRGPGSSVKSQKAHQNASAPEGSGRRSGGTAKRSVNQRRSGQPRSGGGSSGRSASAGH
                     VDDDGTASNDNTIENDNTNQNDNRE"
     gene            70426..71361
                     /locus_tag="PLZ72_00380"
     CDS             70426..71361
                     /locus_tag="PLZ72_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HNA34780.1"
                     /translation="MSATVEWVETTGKTVEEAKEAALDRLGVDEQDAEFEVIEEPRSG
                     LFGRTRGVGRVRARVVPRAPRSKPERRKRSRSDRGGRGEGEKRTGTDAASSGEDKATA
                     SATGGAKPARKASASAQERSQGKADSGRGGSSGRGPSSKSGSGARPAPPDKEREVMDT
                     AEQCDVVTTFLDGLGRAFGLEVTANAQIEDDVLRASIDGSEVGLFIGPGMATLDAIQE
                     ITRNVLQRHAEGREYAKVVVDVAGVRQMRRDRLAAFVTDAARQVLDDDVEVVFEVMSS
                     PDRKVVHDTVAEIDGVASSSIGESPRRRVVLSAAG"
     gene            71424..72125
                     /locus_tag="PLZ72_00385"
     CDS             71424..72125
                     /locus_tag="PLZ72_00385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34781.1"
                     /translation="MPVPRSGSVSESRAEQSPATDAALDRVLMRSRELGFLGPGPTAA
                     HIHHAAAFLPGIDDLESRQGPDRVGELTLVDLGSGGGLPGLILAVRRPGIHVVLIDAI
                     RKRTDFLEWAAAELGVDERVEVRRGRAEELAREPDLRGSAEVVTARSFGPPAVTAECA
                     AGFLSGPGARLLVSEPPTGSEDRWPAEGLLRLGMQPGRLWYAEGATVMAVDVITACAE
                     GIPRRVGVPARRPLF"
     gene            73381..>73519
                     /locus_tag="PLZ72_00390"
     CDS             73381..>73519
                     /locus_tag="PLZ72_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA34782.1"
                     /translation="MAVANQKGGVGKTTTTVNMGAALAELDHRTLVIDLDPQGNATTG
                     LG"
     assembly_gap    73520..73619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <73620..74285
                     /locus_tag="PLZ72_00395"
     CDS             <73620..74285
                     /locus_tag="PLZ72_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA34783.1"
                     /translation="RTLVIDLDPQGNATTGLGINYRTLDHTMYDVLLHDVALEDCIEP
                     TEVRNLFVAPASLDLAGAEIELVPAFSRESRLKRAVADVIDDYDFILIDCPPSLGLLT
                     VNGLVAATEVMVPIQCEYYALEGLAQLTRNVELVRTNLNPELEISGIVMVMYDSRTRL
                     AEQVVSEVRGHFGPKVCRNMIPRNIRLSEAPSYGQPITTFDPSSRGAVAYRELAREVS
                     GGA"
     gene            74275..75195
                     /locus_tag="PLZ72_00400"
     CDS             74275..75195
                     /locus_tag="PLZ72_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNA34784.1"
                     /translation="MARKSGLGRGLTSLIPAGAGEDPDAVAAGLSEVPIESIRPNPYQ
                     PRRAFDEESLEGLSDSIKELGVLQPVLVRELDDGYELIAGERRLRAAKRAGLRTVPVV
                     VRTADDVSSLEQALVENLHRQDLNPLEEAAAYQQLIEDFSYTQEQVAQRVGRSRSAVT
                     NLLRLFQLPPAVQRLVGEQLISAGHAKAILGHPDRAYQEALAKRTVAEALSVRDVEQL
                     VRERIELENSLDGSAANGAAATDEPTSGGTGASEPTGLRPPGLLELEELMADHLDTRV
                     SITMTAKRGKVLIEFANLEDLERIYRRIID"
     gene            complement(75413..76399)
                     /locus_tag="PLZ72_00405"
     CDS             complement(75413..76399)
                     /locus_tag="PLZ72_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNA34785.1"
                     /translation="MADLQRRLAAAGHSPGDPAGEFGESTAAAVSGFQQQAGLRSDGV
                     CDTATWAALVESEFRLGDRLVCLRSPMMRGNDVAELQLRLGALGFDAGRVDGIFGPGT
                     RAAVGDFQRNAGLVADEVCGPDTVEALMRLEGRAGRATVTAVRERDRLRRTMRSLTSL
                     RVAIGSDTDLQPVTTRLAAALQSAGVTTVLLDGDWSAQAAEANDFAADVYLGLSLAPA
                     TVIEASYFEVPGFASTGGRHLAELVVRELPASPGWGIGAVTGMRLPILRETRSSAVLV
                     QLGDRAMVDRNRDLIIASLQRALDVWASDPVAEGPSATSETPGPARYPGVAS"
     gene            complement(76530..76853)
                     /gene="trxA"
                     /locus_tag="PLZ72_00410"
     CDS             complement(76530..76853)
                     /gene="trxA"
                     /locus_tag="PLZ72_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA34786.1"
                     /translation="MGSISQLTESTFDEEVASSTEPVLVDFWAEWCGPCKMVAPILEE
                     IATEQAGKVRVAKVNVDENPGLATKFNVMSIPTMIVFKDGQEAQRLIGARGKPQLLED
                     LTPYL"
     assembly_gap    76895..76994
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(76998..77960)
                     /gene="trxB"
                     /locus_tag="PLZ72_00415"
     CDS             complement(76998..77960)
                     /gene="trxB"
                     /locus_tag="PLZ72_00415"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNA34787.1"
                     /translation="MADLRNVVIIGSGPAGLTAAIYTARADLEPLMIEGEPSSTGDQP
                     GGQLMLTTEVENFPGFPDGIMGPELMGNLRQQAERFGAEIHTAKVSRVDFSERPFRIW
                     VGDPDADEPTHLARSVIIATGASALMLGLDSEHRLLGHGLSTCATCDGFFFRDQDIVV
                     VGGGDSALEEAMFLTRFGRTVTVVHRRKELRASKIMQDRAFANDKITFVWDSVVDEIL
                     GEDRVEGVRLRNVVTDEVTDLPATGVFVAIGHRPNTELFTGQLPVRDNGYLVTLDGTS
                     RTAVDGVFACGDVQDDRYRQAITAAGSGCMAAIDAERWLEAQEH"
     gene            complement(78010..78354)
                     /locus_tag="PLZ72_00420"
     CDS             complement(78010..78354)
                     /locus_tag="PLZ72_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNA34788.1"
                     /translation="MSETSASSGTPSTTTPGRPASGGGDWTDQVTDLIVENVDKVRSH
                     TTGPILEVSKGMVYAIVALILGLPTVVLALVGTVRLLTIFIPAWAVYLLFGIIFVLVG
                     VVLWSKRGRLPT"
     gene            complement(78405..79313)
                     /locus_tag="PLZ72_00425"
     CDS             complement(78405..79313)
                     /locus_tag="PLZ72_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34789.1"
                     /translation="MNDRRPDDSESPQTTPATPGSRRVPRDGTADEWVSAVLDGEADA
                     EESAAVRADPVLLARLARFQDLRDRHRQLALDPPPSPADVDQRVAAALAEMGATSGSG
                     HRPDLVASGHARPERSTRVPRGRSWVAIAAAVVLVVVAAGVAVRATTGRSGTGEHLAD
                     SATEVTGEGSGSLEPPTSTTSPPFTSPAPAGASGAGTSSEESAPSGSPTNSADRSAPV
                     VSDERLAELPDLGTHADTAAMVAATPTSTLDSLPLRDPATLPCRTGGPDAVRVAVAVV
                     AGSPALVILTGDTVRAVSVPGCLPLP"
     gene            complement(79310..79930)
                     /locus_tag="PLZ72_00430"
     CDS             complement(79310..79930)
                     /locus_tag="PLZ72_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA34790.1"
                     /translation="MGRTSQGGDGPSDEDLVAAARRGDRAAMDQLLRRHHDRILAVCH
                     RLCRNPADAEDAAQDALIAIVRALDRFDGRSRFSTWAYRVTTNSCLDELRRRRRRPVT
                     VDATSVPEPAATDVEPADAAVRSEVRSTLVEVLERLPEEFRVPLVLRDVADLDYASIG
                     EILDLAPGTVRSRIARGRARAAALLDGNPTAGADVEVPDPTAMDPP"
     gene            complement(79937..81505)
                     /locus_tag="PLZ72_00435"
     CDS             complement(79937..81505)
                     /locus_tag="PLZ72_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA34791.1"
                     /translation="MDETQVGSGPIDLTRGSLPTGSLAGTTLGGRYRLDRIIGAGGMA
                     QVWEGTDSVLGRRVAVKVLHPHLADDESLVRRFRQEAIAAARLNDPGIVGVYDTCSDD
                     GHEAIVMELLQATTLRQLIDERGTIDPDTTLRIGLRLLDALESAHRAGLVHRDVKPSN
                     VLLCDDGRVKIADFGIAKADDQTELTRDGSLLGTASYLAPEQLTGTDVDARADLYSLG
                     LVLYECLTGRVPFRGDTGAAVALARLHTTPTDPRRIRSDIPPRLADAVMRALERDPDR
                     RFDSAADFRAALLHADVDVPAAVPGPPIEPPAVTTVEHDPPGFGRSERRWLLPAFLIL
                     LIAVGIIVAGLLFRESRQPDTAGPTTSSTVAPAEPLPIASVSSYDPQGSGTSGENDSL
                     AARATDGDDLTAWRTETYDQPDFFGDKTGIGLVIHLAGRSRLENVRVSGSTNGWSGQV
                     YVLDGDPDPAGPGDLHPSATLTDVRGPVSVDLGGATGTTVLLWITDLGDPTDGSRHRV
                     EIQEVVPNGSPAPS"
     gene            complement(81517..83658)
                     /locus_tag="PLZ72_00440"
     CDS             complement(81517..83658)
                     /locus_tag="PLZ72_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HNA34792.1"
                     /translation="MSARSVIARSVPEHTVRRRIRLLLCAVAISTAVVAPAGSLSAVP
                     RQVAGGSAAAPSTSIVVTAVTPWVDPDGDFRIGMRVDGPLPVDAVLTTQIHQRLQAAR
                     TTTLRGAVERVITTDQAGPPLQSPHDRTVASLGDAATGIVVDIPIRGTRSGDPERVLL
                     PNPGIHPVTVSVADAAGTELASTIVFLNRLPDTMPVSRDGSPAAVSLSVYALVDGPAA
                     LRPSGQADIDPATREQLSALASTLESGVPGPFTLAVRPNLLDALDRSEDPADRNILRV
                     LRSATSGSTPATVARMPYVDVDVGSLSESADGRGEVLRQIALTDTTVQRVLGAEPVGT
                     TWYDGDSVTEAGLPFLEVLGARRAAIPVDSLRLHDPRSAPDGARSLAVGLPPSTLTAT
                     APDESLSRLLDDGANGSDDEPGPGQRANQVVTTLMATWYPAAAESGSAGRSEFPGPAT
                     LLRTPVGAEPAVLAALYAALADPGPITLDPGAVPAAAAVVDGEPVTAQVPTDGPTDPG
                     PALREVLDTRRSIDGYRSLAPSATAEAAEWDRIDAQTLHMDMAAADRDTYHRRILADI
                     GVLVAQIEMPPERQVLLTDREATIPLRFRNDLPYAVDVQLWIRSVRLDVGGGDRRIVT
                     LRPGENRVDLPVTVRAPGGTLLRIDASSPDGALALPAVAIPVTSRTISGVGAALSVLS
                     LLFLAGWWTVSIRRERRHRGTERGPGAATDR"
     gene            complement(83655..84383)
                     /locus_tag="PLZ72_00445"
     CDS             complement(83655..84383)
                     /locus_tag="PLZ72_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34793.1"
                     /translation="MTSRQLLRVGEDRFRVGPWHADDRIAYLALPPPPTGPSVDGLNE
                     CLRGIAAAGYSSVITSALHPTEARPFLEAGFTEYDRLRVLSHDLRGLDRLRSRTGGVR
                     LRRARRSDRDDALAVDNRAFPVFWRLDRAAMTEAEKATPVCRFRVALPERDAPRRVLG
                     YAVTGRGSGQGFLQRLAVDPDQSGHGIGSALVLDALCWASRRRCRRVLVNTQRHNTRA
                     LALYRRLGFEVTPTDLLVLHRPVP"
     gene            84510..85529
                     /locus_tag="PLZ72_00450"
     CDS             84510..85529
                     /locus_tag="PLZ72_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNA34794.1"
                     /translation="MTVLVYADERFALHDTGPFHPERPDRLHAVADGLVLAGVDDDVE
                     RVIARPATDEELFRVHPEAFVRGVEGFCRSGRTHLDADTVVSSASADLARLGSGAGLD
                     AIERLRSGDADAAFVAPRPPGHHATASRAMGFCLYNHVAVAAAALAAAGERVAVIDVD
                     AHHGNGTQDIFYRDPDVLYVSWHQHPLYPGTGRASEIGEGPGRGSTWNLPVPAGATGD
                     HYRRSLEELVAPAVEAHGTTWLLVSAGFDAHRDDPLTELGLTSGDYADIVADLLTLVA
                     PGRRVVFLEGGYDLEAIARSTAATVSALYGERLHPEAPTSGGPGDDVVERVTATRDRV
                     ASLGS"
     gene            85526..86917
                     /locus_tag="PLZ72_00455"
     CDS             85526..86917
                     /locus_tag="PLZ72_00455"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNA34795.1"
                     /translation="MIPERARELIDEVRPLAERFAAADARLFLVGGVVRDLFVVAGSG
                     TGPIGDDVDLTTDAEPAAIKAIVAPVADALWTQGEKFGTIGVSIGGRTYEITTHRAEA
                     YTSDSRKPQVAYTTEVEVDLSRRDFTVNAMALEVTHPEPVLIDPFGGLADLAAGLLRT
                     PLDAEVSFSDDPLRMLRAARFIARYGLAPVPELVAAVRDGAPRLEIVSAERIRDELCK
                     LVTVDDPSAGLWFVHDSGLAAMFLPELPAMRLEQDPIHRHKDVLSHTIAVVAKARNEL
                     KVRLAALFHDVGKPKTRSIGGKGVSFHHHEVVGARMTRDRMRALKFSNEMVRDVTQLV
                     YLHLRFHTYQMGWTDAAVRRFVRDAGSQLDELIALTRADCTTRNKKKAEALDRRIDDL
                     EDRIAALQAAEELSAIRPDLDGQQVMAHLGLAPGPEVGRALAHLLELRLDRGPMTEDE
                     AYAALDTWHATQI"
BASE COUNT        12602 a        30566 c        31023 g        12573 t
ORIGIN      
        1 gggcccgcca tggacccgca ggtcaccctg ttcgtcgccg tcgaggttgg tcggtcccac
       61 cgcgaccgcg ccgtcgacga cctgcttgcg gaacgcggtg cgcacgacgc ggccgtcggc
      121 atcgatgtac tcgaccgggc gcccgaccag cacggcgagg gtccggggat cggtgctcat
      181 ggcggcgacg gtagtgccgg tggcggcggt acggtcgggg ccatgtacgt cgtcatgaac
      241 tcgatcgccg tcgacccgga gcacgccgag atcttcgagc agcacttctc gcgctccatg
      301 gacgggacgc tgggctcggt cggtggcctg gtgcgctcca cgctcctgcg cccggtcacc
      361 gccggcgagc cgtacgtcgc ccagatcacc ttcgactccg aggagtcgtt ccagggttgg
      421 ctgagctccg acgccttccg tgccgctcac ggccacggcc cgtcgggcgg ttcgggcagc
      481 tcgggcgacg ggccgaacgt ggtcgcctac gaggtggtca acgaggtcgc cgcgggctga
      541 tcgccttggg agcggcccgc ccgggcgcgc tacagtgcgt cgctcactcg cgcgagtggc
      601 ggaattggca gacgcgcagg attcaggttc ctgtgtccgc aaggacgtga gggttcaagt
      661 ccctcctcgc gcactcctcg ttgtcggatc ggcaacgtac gatcagagcg tggttcactc
      721 gccagcgcct gctgacaccg acgtcgaggt cttccgtcgc caagtcgacc gctggcgaga
      781 gatgacaccc gcccagaggg cggagctggc tgaccacctg agcgtcgacg tcgtcaagat
      841 ggcttcggcc ggcattcgcc gtgaccgacc cgacatctcc gccgacgagc tcgctcgaga
      901 gctggctcgt cggcgctacg ggcgagcgtt cgcggatgca gcgtggaaca gcactgatcc
      961 gacgtgagcg actcgggccc cctgtccctg gtcatacgtg ttgccgccat cctcgacgag
     1021 ttcgacatca cgtacgcact cgggggttcg atggcgagct cgttcttcgg cgagccgcga
     1081 gcgaccgctg acatcgacgt ggcggtcaac ctcgacgctg gcagcggtga tcgactggtc
     1141 gaccggttgc agtcagagtt ctacgtgcca acagcctccg cgcgagccgc tgtccgtgcc
     1201 caggagtcgt tcaacatcct cgctgtcgaa caggggctga aggcggatct gttcgtgctg
     1261 ggcgacagct tgctcgatcg ccgacagatc gaacgacggg ttctcatcgt cctccccggc
     1321 tcaacgcagg gtctgtgggt cacatcgccc gaggaccaga tcctccgcaa gctcgactgg
     1381 tactgccgtg gcggatccgt ctccgaccgc caatggcgcg acgtcctggg cctactgacc
     1441 gtcggcgggg atgcactgga ggtcgactac ctgcatgaga ccgcggcgga agtgggtctc
     1501 tcggacctgc tcgagcgagc ggtcgcagcg gcgagtgagg gctgagcgcg acgtcatgtc
     1561 gcctcggcgg ccagcgccgc tgcgacctcc accacggtcg accgagaggt cgaagatccg
     1621 gtccgccacg ccgacgccgc catgtccgtc gccaagtcgg cggttcgcga ccggctcgcg
     1681 ttctccaccg cctgcgggat ccgaagcggg ccatgatggt ccgatgcgca tcggaatcaa
     1741 cggctcaggt ctcatcgcca ccggcgctcc ggtcggccag atcaccgaac acgccgccca
     1801 agccgaagca gacggcttct cgtcctactg gctcgcccag ctcgcggtgc ccgacgccct
     1861 caccgccatc gcgacgatgg gcgaggccac ctcgagcatc gagatcggaa ccgccgtggt
     1921 cccgacctgg ttccgacacc cgttgatgct cgcagcacag gccgtcacgg ttcagcagat
     1981 catcgggcct cggctggcgc tggggatcgg tctggcccac aagctgtcgg tcgaggccac
     2041 gctgcggatc ccgttcgcga cgccggccaa gcacatggac gagtacctgt cggtcctgct
     2101 gccggcgatg accgacggat cggtcgacgt caccggcgac atctggtcag ggttcaccga
     2161 gggcgcccca ccggctgaag ggcctcccac ggtgctgctc gccgcgatgg ggccccggat
     2221 gttgcagctg gcggggtccc gctgcgccgg gacgatcctc tggttgtcgg gaccgaaggt
     2281 gatcgccgag cggatcaagc ccgcggtcga cgccgccgcg gccgaggcgg gcagacccac
     2341 cccccggatc gtggcgtcgg tgcccgtgtg cgtcaccgac gacgccgacg gagtcaagga
     2401 gatggtggga gcgttcctgg ccggctacaa cgacctgccg tcgtaccgcg gggtgatgga
     2461 cgccgagggt gcgggtggcc ccgcggacgt ctcggtcatc ggtgatgagg cgaccgtccg
     2521 cgccgggatc gccgcgttcg ccgaggccgg agccaccgac ttcgcccccg tcgagatcgg
     2581 gctcgccgaa cgggatcagg tcgccacccg cgacctgctc aaggagctcg ccgcgggctg
     2641 aggatgctca ggtcccggcg acggtgatgt tcgagcgcac cgagttggcg atgaagcagc
     2701 ggtggtggct gtcccggtga agctgggcga gggcggcgtc gtcgggctcg ggcccgctcc
     2761 aggtgatcac cggatgcagc tccacgtcgg tgatggcgag caccccgtcc tccacacgtc
     2821 ccatcgtggc gaccgcgtcg tcgcggtagg tctcgacggg gaacccggcg tcggacgcca
     2881 ggtggaggaa ccacagcatg tggcagctgg cgatggcggc gacgaacgcc tcctccgggt
     2941 cgaccctggc cggatcgccg ttgaggtgcg gcgcggagga cgccggcacc tcgaccccgc
     3001 cgtcgaaacg ccagacgtgg gccctggagt aggtggagtc ggtgggcgcg ccgtcgcggt
     3061 gccactcgac ctgggcgaca tgagtgctca cagacccatc gtacggaccg ccccatcgta
     3121 cggaccgccc catcgtacgg acggccggag tggccacgat cccccgcctc ggcgggaccg
     3181 tcggcgcgag actcgccgcc atgacgacga gcggacggac cacgccggcg ctccggcggc
     3241 tccgggccac ggtgtcggtg gtgctcctgc tggtcggggt cgcgagcgcc tgctccggag
     3301 gaggctccga agagggctcg gcccggggcg ggaacccgac cgactcggcc gccacaccgt
     3361 cgagcagcgc cacgccgacg ggaaccccca gcaccgggtg cggcacctta ccggcgaccg
     3421 agtcggtgga ggctcctccc ggagacgcag tcgtcaccct cggtgaccgc tcctaccggc
     3481 tcggcatccc cgccgactac gaccgggacc gccccgtgcc gctggtgatg aacctgcacg
     3541 gcagcggatc gaacgccctc gagcagacga cctactccga gatggccgca cgcgccggtg
     3601 agcgggggtg gatcaccgtc acccccgacg cgatcggcgg caagtgggag ctggccccga
     3661 ccggcgccga cgacgagttc ctgaccgccg tgttcgacca ggtgaccgcg gggtactgcg
     3721 tcgacctcga ccgggtgcac ctgaccggga tgtcgctcgg agcgtggaag gccgccgcga
     3781 ccgcgtgcgc tcacgcggac cgcttcgcgt cgatcgccct cgtcaccgtc gaggtccacc
     3841 cctcggactg ccctccgatg tcggtggtcg cgttccacgg gacggccgac acggtcgtcc
     3901 cctatggcga gggcgccgat ccgggtgtgg tggtgaccgg cccgaacgcc ggactgcccg
     3961 gtgcccgcgg caacatggcc ggctgggcct cggccgccgg gtgcggcgcg gaaccccgtg
     4021 tccagccgat cggatccgac gtggagcact ggacgtaccc gggctgcccg gacggtcggg
     4081 gcgtggagtt ctactcgatc gagggtggcg gccacacctg gcccggggcg tcgatcgaca
     4141 tcgggccgac gacgcagacg atcgacgcca ccgacatcgc cctcgactgg ttcgaggccc
     4201 acccccgagc ctgactccgg agtggaccgc cggcgctcag cccgcgccgg tggccgccgg
     4261 gtcggcgggg cgacacgccg cacccagagc ggggtccgcg gccccgatcg cgtcgagcgg
     4321 atacgcgacg acgtcgtggg tgaccggatc ggacgcggcg atcaacccga cggcccggcc
     4381 ggtgatgtcg atgacgaccc cgcccgagat cccgggaacc gcgtcgacgt cgaccaccat
     4441 cacctcggtg gtcgcgccgt agccctggcg ggcttccacc gaccgcacgg ttcccgacgc
     4501 ggcgaggaag cgaccgtcgg ggtagcccgc gaccatgacc cgggagccca ggaccggtcg
     4561 gggccccgcc tcgagcggct cggcgccgcc gtcggagagc gccgcgccgt cgatctcggc
     4621 cacgtcgcgg tcggcgacca cacccgacac gtccgcggag ccggccacgc cgtccacgtc
     4681 gacccggtcg gtcccggcga cgacgtgctg gttggtcagg ccgaccaccg tgtcggcccg
     4741 tcgtacgagg gtcaccgtgg cctgacgctc ctggccgcac cccgatgcat ggacccgcag
     4801 caccgaacgc tgaagggctg agctgacggc ggggtcgggg ccgtcacggt cgagcacggc
     4861 tcccgcgacc gcggcgaccg gctggggagc cgccaacgag cgacccgcgt gccacctcac
     4921 gaccagcgcg aagccgagga ccagcagcac cgccatcacg agcacggccc ccggccgccg
     4981 ttccgccacg ccgcgagaga agcgcagcat cgggggtccg ggcaagcccg agggcagaac
     5041 ttcccgtgac gccaccctcc tgacttgtgc attgcatgac gcaagttgcc gtcgtaccct
     5101 tcatggcatg atgcaattcg atcccgccat ggccgacctg gccgccctcc gggagcggag
     5161 gctcgcgacc cccgaccccg gggcccggcc gatcatccga tcgatcacgc ccgacgacgg
     5221 tcccgccctg accgagatgg cactgcggtg cagccccgac tcgatccggc atcgcttcca
     5281 cgcccctctc accggcgccg agccgtcacg cctgaccgcg ctgctgcgcg gtcgcggggc
     5341 gatcatcacg ttggtggccg acgtcgacgg agccgtcgtc gggatcgcca cgctccaccg
     5401 gagcggtcgc ggcgccgccg aggtcgccgt gctggtggag gaccgctggc aggccggcgg
     5461 cgtcgggacc cgcctgctgt cccacctcat gaggcacgcg gcacagcacg ggatccgcac
     5521 cgtcgacgcc gacgtgcagc gcgagcaggg gttcctgatc gaccgactcc tccgcggcgt
     5581 ccccgacgct gctgcgtcct tcgacggtcc cgtcgcgacg ctgcacctac cggtcggacc
     5641 ggcctcgtag ggtggggtcg tgcgccgcgc atcgttcacc gacatgcagt gctcggtggc
     5701 ccagtccctc gagcagatcg gcgagtggtg gtcgctgctg atcatccgcg acgccctgct
     5761 gggcgtgagg cgcttcgagg acttccgcac ccggctcggc atcgccagca acatcctgac
     5821 cagtcgactc gacaccctgg tcgcccacgg catcctcgag cgtcggcgct actccgagca
     5881 ccctccccgc gacgagtacc gcctgaccga caagggtcgg gacctctggc cggtgatgac
     5941 cgccatccgt cagtggggcg acaagtgggc cctcgatctg gaggaccatc ccaccgtcct
     6001 ggtccacgac acctgcggtc acgtctgtca ggcggtcccc cactgcagcg agtgcggcca
     6061 ggagctggag ctcggatcga tccacgtcgc gcccggaccg aacgactccg acccgtcgtt
     6121 catcccgcgc cgtcccgctc ctgggtcgga caggtcctga acggacaggt cctgagcagt
     6181 caggagcggc actccaggta gcgccgccac acctcgtcga ccgcgacggt cgcggggatc
     6241 tcaccgtcgt ggacccgttg ccgcgccgcg tcgagaaccg cccccatggc gggatcggtc
     6301 cgcagatcgt cgcgcagccg gtcgtcgagc atcgcccaca tccaccggac ctgctggtcc
     6361 cgtcgacgtt cctggagctc gccggtggcc gacatcctgg cccggtggat caccacctgc
     6421 tgccacagct cgtcgagccc gtcgccggtc agaccggagc agacgacgac gggcgggctc
     6481 cacgtcggcg atgccggcga catcaggtgc agagcggcgc ggtagtccgc ggcagccaac
     6541 tcggcccggg ggcggttgtc gccgtcggcc ttgttgaccg ccaccaggtc ggccagctcg
     6601 agcaccccct tcttgatgcc ctggagctcg tcgccggcgc cggcgagcat gaggaccagg
     6661 aagaagtcga ccatgtcggc caccacggtc tcggactgcc cgacccccac cgtctcgacc
     6721 agcacgacgt cgaagcctgc cgcctcgcac agcaggatcg tctcccgcgt cgcccgggtc
     6781 acgcccccca gcgagcgacc cgccggcgac ggccgcacga acgccgccgg gtccaccgcc
     6841 aggcgttcca tgcgggtctt gtcgcccagg atcgagccgc cggtgcgcga gctggtcgga
     6901 tcgaccgcca ggaccgcgac ccggtgaccg gccgcggtga ggtcgatgcc gagctggtcg
     6961 atgaaggtgc tcttgcccac ccccggcacc ccggtgatgc ccacccgatg cgcgccaccg
     7021 gtcgacgcca acagctcctg caccagtgcc tgggcgaccg tccggtgctc cgggttctgg
     7081 ctctcgacca gggtgatcgc ccgggccagg acgctgcgat cgccggcgcg gatcccgcgg
     7141 gcgtagtcgc tgaccgggag cagggccgcc atcgacggca ccctaccggg catccgaacc
     7201 ggcgtgatcg tgccccgccg ccgctgtcgc gaaccgggtt caccactctc gcacgcccgg
     7261 ctctagggtg atcgccggga gagggccggc acgatcagta cggatgaccg tacgacacac
     7321 cacgatggcg cgcaggaccc gggaagaacg acgacaggac tacctggatg cgggcgtgga
     7381 gctgctgacc gagcagaccg cggcccgacc cgacccgggg ctcgcgttcg cccacgtccg
     7441 cgtcgccgac gtggccgccc gcgccggggt caccaaggga gcgctgtacc acctgtggga
     7501 ctcccaggag gcgtactggc acgacctgtt ggagttcatg ctcgacgagg gccggctggc
     7561 cgggatgccc gccgtcaccg acgccacccg cgagctggcc gaggccaccg acgaccttcc
     7621 gacaccggtc gagtgggccg accacgtctt cgaacggttc aaggacgacc cgtcgttctt
     7681 cgcccggatc gggatgctgt cgtacctgca cgacggtgga gtccactccg acctcgacga
     7741 ggagttccgc gccagcctgg ccgagttcga ggcgatggtc gccacctcgg tgcgacagct
     7801 cggacggcgg ccccgagtcg ggaccgacct gcgggacttc agcgctgcgg tcgccgcgct
     7861 gatgcacggc ttctgcctgg agcatcgcaa cgacccggac cgtacgcccg aggtcgtcgt
     7921 gcagggccga ccccagagcc tgttcgcgtc gtgcatcacc gccttcctcg acgccttcac
     7981 cgaacccgac accgcccaag aacccgacac cgcccacgag agcgggggga acccctgatg
     8041 gcgcgaccga cggcggcgga gcggcgcctc gactacctgc gcatcggcgc ggatctggtc
     8101 acgggcttcg gcgacgccgg gtccccggcg ggccccctcg aagcgctggc gaacgtcaag
     8161 gtctccgacg tggcccgcca cgccggggtc accaagggag cgctctacca cctctgggac
     8221 tcgcaggacg actaccggcg cgacctcctg cagcacctgc tcgatcaggt ggagaaagag
     8281 ggcctggagc gcgccgagca gatcatcgcc gaagcggtcg acgacgaggg ccggccgttc
     8341 atcgacgtga ccgacctggc cgtgcacctg tcgaccggtt cctaccggga cctgcgcgac
     8401 gacccgcggg cccaggcgca gttcagcttc tacaactacg tccacgatcc acgggttcgt
     8461 cgcctgctgg cgacaggctc gtccggcttc gactcctact acgacctcta cctggtcacg
     8521 tctggtcgcc ggatgcgcga gccctacacc gtcgggcacc tcatcacgtc ggtcaacgcc
     8581 ttcatgatgg gctcggtcat ccgacaccgc atcagccgag gatcggacac ctccgccgac
     8641 gatgcacaga tctacgccac gggcttccgg gccctcctcc tgcacttcac cgaacccctc
     8701 cgcgacaccg aacccctccg gtgacgccgc tctgtagtgc gagatccagg cgttcgcctg
     8761 gatctcgcac tacagaaccg ggacggggac ggagtcctca ggtcgagagg tcgtagccga
     8821 gctggtcggc caggcggtcg agcaggtccg aggcggcctc gtggatgacg gtgccgggcg
     8881 ggaacaccgc ggcggcaccc gcgtcgtaca gcgccgggta gtcctggggc gggatcaccc
     8941 cgccgaccac gacgaggatg tcgggtcggc cgagagcctc caacgcctgc ttgagctcgg
     9001 ggaccagcgt caggtgtccg gcggcgagcg acgacggccc gacgatgtgc acgtcggcct
     9061 cgaccgcctg acgggcgacc tcctcggggg tctggaacag cggtccgatg tcgacgtccc
     9121 agccgagatc cgcgaacgcc gaggcgatga ccttctggcc gcggtcgtgt ccgtcctgac
     9181 ccatcttcgc cagcaggatc cgcggccgcc ggccgtcagc ggcctcgaac gcctcgacct
     9241 tgcgcctgac ctggtccacc gcggcacccc tctctccgag ctcgctgcgg tacacgccgg
     9301 agatcgaccg gatgtcggcg cggtgccggc cgtacacctt ctccagcgcg tcgctgatct
     9361 caccgaccga cgccttggcc cgcgccgcgg cgacgccgag gtccaacagg ttgccctcgc
     9421 ccgaggacgc cgcgttcgtc aacgcctcca gcgcgacccg taccgcctcg gggtcgcgtt
     9481 cggcgcggag gcgctccagc ttggcgatct gcccggcacg caccgccgag ttgtcgacct
     9541 tgaggacgtc gatgtcctcg tgctcgtcca ggcggtactt gttgaccccg atcaccggct
     9601 gacggccggc gtcgatgcgg gcctgggtgc gcgccgcagc ctcctcgatg cggagcttgg
     9661 ggatgcccgc ctcgatcgcg gcggccatgc ctccgagctg gtcgacctcg gcgatgtggg
     9721 tcaacgcccg ctgggccagc tcggcggtga gccgctcgac gtagtagctg ccgccccacg
     9781 ggtcgatgac ccgggtggtc cccgactcct gctgcaggaa cagctgggtg ttgcgggcga
     9841 tgcgggccga gaagtccgtc ggcagcgcga gcgcctcgtc caggctgttg gtgtgcaggc
     9901 tctgggtgtg gccctgggtc gacgccatcg cctcgacgca ggtgcggatc acgttgttga
     9961 acacgtcctg cgcggtcagc gaccagcccg aggtctgcga gtgcgtgcgc aacgacaacg
    10021 acttggcgtt gccgggatcg aagcgctcct tcaccaactt cgcccacagc agccgcgccg
    10081 cgcgcatctt ggcgatctcc atgaagaagt tcatgccgat cgcccagaag aacgacagcc
    10141 gcggcgcgaa ggcgtcgacg tcgagaccgg cgtcgaggcc ggcctggatg tactcgaggc
    10201 cgtcggcgag tgtgtacgcg agctccaggt ccgccgtcgc gcccgcctcc tgcatgtggt
    10261 agccggagat cgagatcgag ttgaacctcg gcatccgctc cgaggtgtag ctgaagatgt
    10321 cggagatgat ccgcatcgac ggcgtcggcg ggtagatgta ggtgttgcgg accatgaact
    10381 ccttgaggat gtcgttctgg atcgtcccgg cgagcttctc gggcgccacg ccctgctcct
    10441 cggcggcgac gatgtagagc gacagcacgg ggaggaccgc gccgttcatg gtcatcgaca
    10501 ccgacatctg gtcgagcggg atcccgtcga acaggacccg catgtcgtag atggagtcga
    10561 tggcgacccc cgccatgccg acgtcgccgg cgacgcgggg gttgtcggag tcgtaaccac
    10621 ggtgggtggg caggtcgaag gcgaccgaca gccccttctg cccggcggcc aggttgcgcc
    10681 ggtagaaggc gttggagtcc tcggcggtgg agaacccggc gtactggcgg atcgtccacg
    10741 gctggttgac gtacatggtc gggtacggac cccgcaggaa cggcgccaga cccgggtacg
    10801 tgtgcaggaa gtcgagcccg tcgaggtcgg ccgccgagta cagcggggcg acgtcgatgc
    10861 cctcgggcgt ctcccatcgc tgctcgtcga cggcgagccc ggtcgcggac tgcgccgcgg
    10921 cggcccagcg ttcccgggcc ccgggatcgg cggcggccga cgaggccagg tcgacggtgc
    10981 tgaggtcggg gacctcgaag cggggtccgt ccgcggtcat gacgacacct ccaggatctc
    11041 ctgcgcccgt tccagggtgg cgagcacgtc gacgccgacg tggacgaact cgtcgactcc
    11101 ggcggcctcc tcggcggcgc ggcggtcgcc cgggttgccg gccaggtaca cccgacgtgc
    11161 ccccgcggcg cgcagcgcct cggcggctgc gaccgcgtgt tcggcgtaga cggcgtccga
    11221 cgagcagatg cacaccaggg cggcaccgct ggcggcgaag tcctcggcgg catcggccgc
    11281 ggtggcgaaa ccggtgccgg cccctcgacc ggaggtgacg gtgcgcagtc cgccggcctc
    11341 gaagaggttc ttggcgaacg tcgcccgggc ggtgtgggtg gccaccggac cgaggttggc
    11401 caggaacacc accggctcgc gacccgcggc gcgggcggcg tcggctcggt cgcgcagctc
    11461 ctcgaagcgc tcggcccagc gaaccgggac cagcagcgga tcgacgccgt cgggttccgg
    11521 tggaggcggt ggcggcgggt cctcgccgag gtcggggaac tcgctgaccc cggtgatcgg
    11581 agcccgacgg gtggcgatcc gcgccaggcg ggcgtcgcgg acatccgcga tgcgggcggc
    11641 gagggtgccg tcgagcagca ccgtcggcat cccaccgacc gactcgagct cctggagcag
    11701 acgccacgcc aggtccgcga gctcgtcggt gaggctctcg acgtaaccgg aaccgcccgc
    11761 cggatcgatc acccgcccca ggttcgactc ctcctggagg aggatctggg tgttgcgggc
    11821 catgcggcgg ccgaggtcgc tcgggtcgcc gagcaggacg tcgtaggggt gggcggtcac
    11881 gccgtcggcg ccgccgatgc cggccgcgaa caccgccgcg gtggtgcgca ggaggttcac
    11941 ccacgggtct cgacgggtga gcatgcggcg ggcctcgacc acgtgcagca ccggtgcggg
    12001 agcgtcgccc gacacgccga cggcaccgag caggttggcc cacaggcgac gggcggcgcg
    12061 cagcttggcg acggtggcga acacgtcctc gtcggccgcc agctccagct ggatctgtcc
    12121 ggcggcctca tccgcgctga gccccgcggc ggacatggcc cgcaggtagg ccgccccggt
    12181 ccccagcatg atcgccagct cctgggcctc ggacgcgccg gcctcgaccg ccgggagggt
    12241 gctgaccgtc acggaccgca cccgggggtg ggtggcagcg gtgcgcgccg ccagtgcgcc
    12301 cagctcggtg agaagggcct gcgggtcctg tccggccccg ccccgggccg ccagcgcagc
    12361 gagcgggtcg gcaccgaatc cgccgacggc ctgggcgggg tcgatcccgc gctggtccca
    12421 cagggcgatc agtgcgtcgg cggcggcggt gaagtcgaac cccggccgca gcacgaccgg
    12481 ggccaggtcg aggtggacgc cggaaaggac cgcgtccagg tcggtcgcct cggtccaggg
    12541 cacctcgagg ctggtgaccc cgcgctccag atcggtgagc gcccgggcgt tggcggcggc
    12601 ggggtccggc cccccgacga ccgatcggat gtcccaggcc acgtccgggc gtggtgctgg
    12661 gtgcgtgccg cgggtgaacg gtgcctggcc ggggaagccg gcctcgtcgg cggctcccgg
    12721 ggagttgtcg ggggtgtaga gcggctggac cgtgacgccg tcggcggtac ggccgaccag
    12781 cttggtgaac ggtgccccct tgagcacctt gtcgaccgcg gcgagccacg cttcctcgtc
    12841 ggccggatcg aagcccccgg ccagggcggt cgcggccgtc ggggccgtcg tcggttcgga
    12901 cacgctgatc tccgtcgtct gggtcccggt cgaggggacc tccggcgtgg cggcagctcc
    12961 gctcgaccgg aacgccgtcg agcgaggtta ccgaacgtgc ggccgacctc ccgaggccgg
    13021 gagtcgctgc gtcgcgcgcc aggatggggg catggacgac cgacccgacc cgatcgagct
    13081 ccccgacggc ctggagctcg cccgcaccac cgacgtgttc gacgaggcga accaccccgg
    13141 cggcctgctg cgggcgcacc aggtggcggc cggggtgtgg gcccggctgc tggtccacac
    13201 cgggagcctg cgcttcgtgt tcgaggacga gtccgaccgg ccccgcaccg tcagcgccgg
    13261 cgggtcccag gtcatcccgc cgcagcgacg tcaccacgtc gagttcgacg ggccggccac
    13321 gttcgccatc gagttccacc ggcctcccgc caccgccggg ccggccgagg gaccggccga
    13381 gggggcggag agcaccggcc tgcagccctg agatcggctc ggccgggatc cgacccgatc
    13441 gggtcggttc ggctcagctg ccgccggagt cggcggccgg ctccgggtgg tggaacacca
    13501 cgagggtgcc gatgccggcg accagtccga gcacgcccgc gatgagggcg ccgcgccagt
    13561 cccgggtcag gtcgtcgagg atgccgaggg cgtggtcgag ggaccactcg cccgggccga
    13621 gcgccccgat gccccaggcc aggaacccga acaccatcac gtactcccag ccgttcttga
    13681 acatgaagaa cgacccccgg tgatcggtgc gggccgcgac caccatgatc ccgatcagcc
    13741 cggcggccgc gaacggggtg agcaggccga cggtcagcag cacgcctgct cccatctcgg
    13801 tgctggccgc catccaggcg ttgagccggc cgggcttcat gcccaggccc tcgaaccagc
    13861 cggcggtccc ctcgatcttc ccgccgcgga acaccttgtt gtagccgtgc aggaacagcg
    13921 tcacgccggc cacgacgcgc aggaccagca tgacggtgtt gaactcggtc gcgaaccctg
    13981 cggcgtggat catggtcacc tccggtcggc gatcgtaggc gcggggtaga ccacagcacg
    14041 atccccgcgc acccgccggg gccccgagca cccgaacaac cgaacaaccg aacaccagga
    14101 gacgcgtcga tggccgagtt gaggaccttc cccgaggact gggaccgcgc gctggccgtg
    14161 gtcgcccacc ccgacgacct ggagtacggc gccgcgtccg ccgtcgcccg atggaccgcc
    14221 cagggcaagg aggtcatcta cctgttggtg accgacggcg aggcgggcat cgacggcatg
    14281 gcccccgagg agtgcgggcc gttgcgccgc gacgaggaga tcgcgtcggc cgcggtggtc
    14341 ggggtgcaca ccgtcgagtt catgggccgt cccgacgggc tggtggtggc cgacctggac
    14401 ctgcgccggg acctggccgc ggcgatccgc cggcaccgcc cggacgtgtt gatcgccacc
    14461 aacttccgcg agcactgggg cggcgagggc tggaaccacg tcgatcaccg caacaccgga
    14521 ctcgcgttgc tcgacgccgc tcgtgacgcc ggcaaccggt ggctgttcac cgacgccggc
    14581 gaaccgtggt ccggcacccg cttcgcggcg ttcctggggt cggacatggc cacccacggc
    14641 gtcgatgtcg gggactggat cgacaagggc gtcgagtcgc tcgaggcgca ccgcacctat
    14701 ctggacgcgc tcccgccggg gacgccgggc accgacccgg agccgttcct gcgaggcatg
    14761 gccgagtggg ccgggccccg cctcggcgtc gacctcgccg tcaccttcga actcatctcg
    14821 atataggggt cctcaacgac acaccttgtc gcaatccgga ccccagaaat ccgagcccgg
    14881 acccgtcagc gggcgccgcg gagcagtcga cccgggcgag cgccctggtc ctcgccgtcg
    14941 acgagggtga cctcgccgga cttgaccgtc gcccggtagc cgtccgctcg ctggatgaat
    15001 cggcgggcgt cgccgggaag gtcgtgcacc atctccggtc gccgcaggct gagagcgtcg
    15061 aggtcgatca ggttcacgtc cccgacccgg cccgctgcga gcacaccgcg atcgcccagc
    15121 ccgtagagcg acgcggtgtc cgaggtgagg gtccggaccg cctgctccac cggcagcccg
    15181 tcggcgcgat cgcgggccca gtgcgtcagc aggaacgtgg ccgctgatgc gtcgcaggtc
    15241 gttccgcagt gggcgccgcc gtcgccgagg ccccaccgcg tgaccgggct ggacagcatc
    15301 tcgtgcactg catcgaggtt gaagtcgctg tagttgagca gtggtcgcat gatcagcgcg
    15361 tcgccgtcgt cgccgaggag gtcgtcgtag tacaactccc acgggctgat gccccgggcc
    15421 cgagcccgag cgccgatcgt ctggtccagc gacggctcgt agtcgggaac cgcccccatc
    15481 gtgaccaccc gctccgggtc gaggaactgg gccatggccg ggtccatctc cccgacctcg
    15541 gccaggagcc gctcccggaa cgccgggtcg cccatcgccc ggacccgctc cgcgatcgac
    15601 agcagaccca cctcggacca cgacgggcag tacgagaacg ggtggaacgt ctgcaggccg
    15661 agcagcaggt tgatcggccg ggacgcgacc tgcgggatga ccagcgagcc ctcgtcctgc
    15721 gcctccgcgc tgagctcgag catgcgacgg taggcgagcg ggtcgtggtc gttctgcgtg
    15781 agagcgaaca cgatcggacg gtcgatcgcc gcgccgagcc gacgcatcca cgccatctcc
    15841 gtgtcgggcg cggccaggtc ctcacccagg gcacccgccg gtgccagctc gaacaccccg
    15901 gtacccgcct cggcgagggc ggcgccgatg ccgaacagct cgtcctcggc ggcgaaggtt
    15961 ccggggaccg gttcgccgtc gatggccatg tgggcgatgg tccgcgaggt ggagaacccg
    16021 agcgcaccgg cccgcaccgc ttctccgacg atggccgcca tcatctcgat gtcgtccgcg
    16081 gtggccggct cgttgcgggc gccgcgctcg cccatcacgt aggcccggac cgcaccgtgg
    16141 ggaacctggg tgccgacgtc gagcatcttg ggggcccggt cgacggcgtc gaggtactcg
    16201 gggaaggtct cccagctcca gcggatcccg tcggacagcg cagctccggg gatgtcctcg
    16261 acgccctcca tcagcccgat cagccagtcg tggcggtccg ggtgcaccgg ggcgaacccg
    16321 accccgcagt tgcccatcac caccgtggtc accccgtgcc agcacgtcgg ggtcagcatc
    16381 ggatcccagg tgacctggcc gtcgaagtgg gtgtggatgt cgacgaatcc cggggtcacg
    16441 agcagcccgt cggcgtcgag ctcccggcgg gcggggccgt cgacgcgacc gacctcactg
    16501 atgatgccgt cggtgatggc gacgtccgcg gtacgggccg cggagcccgt cccgtcgacc
    16561 accgatccgg cgcggatgat caggtcgtgc acgttccgtc ccccttgtcg gtccccccga
    16621 gcgatccacc tccggtggtc cgccccgcca ttgtgcctga ccgggtcggc cggcggattt
    16681 ctggggtccg gattgcgaca aggtgtgtcg ttgagcaccc ctatatcgat gttgttcagt
    16741 tgggggtggg acgcgccgac gctggtggga cgatgccagc cgatcgacag accgaccccg
    16801 acgcgccctt gaccgaccca tccggccccg acctggccgg cctcagcgcg tccatcgacc
    16861 ggctgaccga ccacgcccac gactggagca cccaacggct gctcgacgag gggctggacc
    16921 tggtgcgcga cgcctgctgg gccgacgcag cggcgctgtt ccgcgtcggt gcccgtcggg
    16981 ccgccgcggt ccacgcccga ccgtcggttc ggtcgcgacg tactcgcccg ggcggggtgt
    17041 gcgcgccggt gcccgacgcg gtcgacaccg gctggttccc ctggggcctg gccccgttgg
    17101 ccgcggaccg gttcgtcctc atcgacgacg cccgcgccct gcccgccgac cccgacggcg
    17161 cccgcaccct cggtgaactg ggcgtgcggt cgtgcctgca cctgccgttg cgtcagcggg
    17221 gccggaccgt cggagcgctg caggtgttct ggtcagaacc ccgcctggcg tgggacgacg
    17281 accgtggtcg tctgctgcgg accctgggtc ggttcctgct cacctgcgac ggtcgcgtcc
    17341 ggacctgatc cgcgtcagtc gctgcgaccc tcggtcggcc ccgtctcccg atcgtcgacg
    17401 ctgccggcga cttgatcgga gctgtcggcg gcgtcggctg acgaggcgcg atcagggccc
    17461 gtagcggggt cgagcgggat gccgtcgtcg tccatccgtc gcaccccgac cgcggcgtcg
    17521 ccgtcgccca tgcgcagcat ccggcgcatg gcccggcgca cgccctggtc gccggactcg
    17581 gcgcggatct cctccatctc ggtcaggtgc gagtcgacga cctcgtggcg ctcgagaacc
    17641 gtcgagacga acgcctgcac cactccggca gcgggcaacg ccatgatcgc ccccatcggt
    17701 ccgagcaacg tcccgccgac gatcgcggac ccgaacgcca ccgcggggtg caggtccatg
    17761 gtctgtgcgg tgatcttggg ggcgaacagg tagttctcga cctgctggta cacgaggatg
    17821 aacccgagca cccacagcgc gtcgatcggg tcgttgagca gagcgaccag caccggcagc
    17881 gcgccggcca ggtaggtgcc gatggcgggg atgaactggc tgatcagccc gacccacagc
    17941 gccagcgcca acggcgaggg cacaccgacg atcgacagga acacccacgt gcacgccgag
    18001 gacaggacag ccagcaacaa ccgcgagtag atccatcccc cggtcttctg gatggccagc
    18061 tcccacagca gcagcacggt ccgctggttc cgctgcggca ggatcgagca gacgttgcgt
    18121 cgcagctgcg ggccctgcga ggtcatgtag tagctgaaca gcccgatcgt gaacgcctgg
    18181 aacagcagtc cgaacagcgt gccggtgacc gacaggaccc gaccgccgag gctcttggcg
    18241 atgtcggtca ggtcgtcctg gtagctctcg atctgcgtgg tgatgtcgtc ggtggtgatg
    18301 tcggtgtcga aggtgttgtt gatccagtcc gcggactcgt ccaggtacga aggggcgcgg
    18361 tcgacgagat cctcgacctg ggtgacgacc aggtcgccca tcacgaacag gaacaggccg
    18421 ccgaccacga acagggccag gaagcagaac agggtggcca ggctgcgctt ccagccccgg
    18481 tcggccagga agtcgaccgc gggttcgacc gcgaacgaca ggaacagcga gatggcgacg
    18541 atgagcagga aaccctggag ctggatcagg atcgaccggc cgaactggta cgccgcgacc
    18601 gacaccgcga cggcgacgat cgcccgccag aaccaacggg ggagcgcccg gccctgcccc
    18661 atgtgcaaga cgagctgcgg cacctcgacg tcggggtccg cggcgggctc ggtcgatccc
    18721 tcgggtggac gtccggcggt gaggtcggtc acccgcccaa ggttggcacc ggcgccttgc
    18781 ctgttcgcgg accgctcagc cgggaccgaa catcatcagc cactgctcgg cgctgcgcgg
    18841 cttgaacacc acgttgcggg cccgggtgtc gcccatcagg tccgaggact cgaccgagta
    18901 ctggtggccc gggaacagca gggcgtcgtc gggcactcgg gcgagggtgc cggtcagcga
    18961 gtggtacagc gcctcggggt cgccgccggg caggtcggta cggccgcacc catcgaggaa
    19021 gagcgtgtcg ccggacacca ggcagttgtt gacgtagaag cactggctgc ccggcgtgtg
    19081 gcccggggtg tggatcagct cgacggggat gtcaccgacc atcacggtgt cgccggcgtc
    19141 gtgggtcacc aggtctgcag ccgtcagctc ggtgaccttg agcacccact cggcctcatc
    19201 ggactggacg tggatcggca cccccgccac ctcgacgagc tcggccgcgc cctcgatggt
    19261 catgcccatc atcgatcccc cgcagtggtc ggggtggtag tgggtgacca gcgcgccgct
    19321 gagcgtcatc ccgtcggcgg ccgcgaccgc gaccaggtcc gacaccgcgt aggccgggtc
    19381 gaccacgacg cactcgcccc ggacacggtc gccgatcagg taggcgaagt tgaccatctg
    19441 ggtcgcgacc gggtcgccga ccgcgaagtc ccgaccggcg agcagctggc ggaagtagaa
    19501 gcggtcgtcg aacgcacccg ggccgtacgt tcccgcggcc cgggggtggt cggtcggttc
    19561 gggacgggga tcgctcacgt ccggcacgct actgcggccc tccccgttca cccgagcgga
    19621 gccagcccgc caacaggtcc ctgtaggcgc ggtcgtatct cttgtagtcg gcacgcagcg
    19681 cgtcgccggg ccaacgccgg tcgagcagct cccggggcag cagcggatcg gcgttgaaat
    19741 ggcgcaggac cgcggcggac agcacgaacc ccggagcgag ggcgtcgacg tcgccggcgt
    19801 cgaggcgttc gaggagtcgg gccatctccc gtcgcaggtc gacggcgtgc tccccccacg
    19861 acggcaggtc ccacagccgg gtggccaggt cggcgtcatc gtcggggtgg acgcggaacc
    19921 agcgggtgaa cggcgcgacg acgctccggg attcggttcg gtcgtcgtcc atcggcaggt
    19981 tgtcgggacg cagccagacg ccgtcgcgca gctcggccat ccgcagtcgt cccatcgccc
    20041 ggcgcagggc cgcccggtcc gcggcggaac gaccacccgg ctcgaccacg gcctgggtcc
    20101 aggcaccgga ccactcgagc gtggtggcgg atcggctggc ctcctggcgt cgttggcgct
    20161 cggcgagctc accggtcagc tcgtaccagc cgtcgtcgtc acgagtggca tcgccggctg
    20221 cgaccatccg cgacagggcg gtgcggaccg ctccctcctc cagtccgaac agagctccgg
    20281 cgcgaaccag cgccggagcc ggcatccgag gggggtgggt ccccaacagc gtcgacgcca
    20341 gcaccgagcg ggtggtcaac ggacggtcgg ccaccaccgg gggtctcgag gtcggtgggg
    20401 ccactccccc acgctagacg gggactgacc attacactcc tggttcagtt gtcactgact
    20461 gtgtaatctg gttgtgtgac cgccaccgtc cgtgccccgc tcacgatgct gccgccgtcg
    20521 gaggccctga ccgacgtgct gcccaccgag cgcctgggcc ccaccggtcg acccgtcggg
    20581 gagctgcgcg ccgagctgta ccgggtcccg aacgtggcca acgtcggtca cgtcgccggg
    20641 gtgtggatcc agtcggtcgg agtgctcgcc ctggctgcgt ggtgggggaa cccgatcggc
    20701 tggatcgcgg cgttcctcct gatgggacgg gcgatggccc gcttcgcgat cctcgcccac
    20761 gaggcgtcac accgcctgct gttcacggac cggcggatca acgactgggt gggggcgtgg
    20821 ctgctggcgt atccggcctt cgtcccgttc gacctctacc gccgatccca catggcgcac
    20881 cacagggagg agttcggacc caccgaaccc gacctgatgc tctacaccgg ctacccgatc
    20941 acctcggcgt cgtggcggcg caagctgacc cgcgacgccg acggcaacag cggctggaag
    21001 aacctgcggt cgttgctgct cgcggtgcgc tccccaaccg cccggcccgt ggcgctcaag
    21061 atcctgggct ggcaggtcgt gctgttcgtc gggttgtggg tcggcctcgg gcactggtgg
    21121 gtgtacccgg tgttctggct gctcccgtgg atgacggtgt ggcgcgtgtt caaccggctg
    21181 cgcgccgtcg ccgagcacgg gggcatggag gcctcgccgg accgacggct gaccacccac
    21241 cacgtccggc aggggtactg ggcccggttc tggatcgtgc cgttcaacac cggctggcac
    21301 ctggcccacc acgtcgacat cggcgtgccg ttctccaggc tgcccgcgct gcagcgcgag
    21361 ctcgaggccg ccggctacat caccccggct ctggagtggc cgagctaccg ggcgctgtgg
    21421 cgccacgccc gcagccgccc ggacgccagc ccttccgact gacggtcccg tcctgatcgg
    21481 cccgaccggc tccggcactc aggcggtggc ggccatcgcg gcacccaccg cgtcgagcac
    21541 cagcgcgacc tcgtcctcgg agatcaccag cggcggcatg aaccggatca cgttgcccca
    21601 cgtgccggca ttcatcagca gcacccggga ctgctcgcgg cagtgggcga tgaccgcggc
    21661 ggtccgggct gcgtcgggct cgcccgtgac cgggtcgcgg aactcgatcg ccaccattag
    21721 tccgggaccg cggacgtccg cgatcaccgg gtgcgcgtcg gccagggtcg acaggcccgc
    21781 tcggagctgc tcgccgcggg cgtcgaccgc gtcgaggaac ccgtccgcgg tgagcacctc
    21841 gatggtggcc aacgccgccg cgcagccgat cgggttgccc ccgtaggtgc cgccgtgcga
    21901 cccgaccggc cagcgctgca tcagctcggc cgacgccccg atcgccgcga tcgggaaccc
    21961 cgacgcgatg cccttggcca tcaccaggat gtcggggcgc accccggagc gctcgacgta
    22021 gaacatgtgg ccggtgcgtc cgaagccgca ctgcacctcg tccgcgatga acaggatccc
    22081 gtgctcgtcg cagatctcgc ggatgccgcg caggaaccgg tcgggggccg ggtggtatcc
    22141 gccctcgccg agcaccggct cgaggatcac cgccgcggtc tcggccgggg cggtctggct
    22201 ggccagcagg aaccgcaggt cggccaacgc ccggtcgacc gcgtcgttct cggtcaggcc
    22261 ggtgccgacg gtgtgcggga acgtcgaggg gaacacgccg gacggcagcg gggtgtgccc
    22321 ggcgcggtag ccggtcttgg aggtggtcat cgccatcgcc aggtgggttc gtccgtggaa
    22381 gctgccctgg aacacgatca cgttgggtcg gccggtggcc tgcttggcca gcttgaccgc
    22441 accttcggtg gcctcggctc ccgagttggc gaagaagaag gtgtcgatgt cggccggggc
    22501 cagctcggcc aggcgggcgg cgagcggttc gagcaggtcg tggcggtagc agttgacctg
    22561 ggcgtggacg aaccgctggg cctgttcggc gatggcggcg acgaccgtcg ggtggcagtg
    22621 gcccgtggag gtgacggcga tgccggatgt gacgtcgagg taccggtcgc cgtcgacggt
    22681 ggtgacccag cagccctccc cgctggcgac ctgcaggtcg gtgacctgga accacaccgg
    22741 cgccaggtgc gagcgcggcg tgacggtgga cggatcgacg atcggttcgg agcccatggg
    22801 tgccgatgct acgacggcgg ccggcgccgg ccccggggcc acggatccca cttcggtgag
    22861 cgaggggacc gccggtagcg tcggagtcca tgacctcctc accgtctccc gccggatccg
    22921 tcgacgcccg tcgcgtcgcg ttcctggggc cggtcggcac gttcaccgag caggcactgc
    22981 accgggaggc gggcctggcc gccggcgagt tggtcccgat ggcgaccatg gccgacgtgc
    23041 tgttcgcggt caacgagggc gacgtggatg ccggtgtggt gcccatcgag aacgccatcg
    23101 agggcacggt caacgccacc atcgacaccc tggcgttcga cgtcgatctg gtgatccagc
    23161 gtgagctggt cgagccggtg agcatgcacc tgctggtacc gccggggtct ccgttggacg
    23221 gcgtccgcgt catccactcg atcccggtcg cgaccgccca gtgtcgggcg tggctgcggg
    23281 cgaacctgcc cgacgccgag gtccgtccgg ccaactccac cgccgaggcg gccgcgatct
    23341 gcgcccgcac cgcggacccg gcggtggcgg ccatcgccaa cgcccgcgcc gccgaggtgt
    23401 acggcttgga gcgcgccgcg tccgacatcg agtcgcaccc cgagaaccgg acccgcttcg
    23461 tcgtggtcgc ccgctcgggc atccccgctc cgaccgggca cgacaagacc tcgatcgtcg
    23521 tctaccagcg cgccgatcgg cccggttcgc tgctggcgat cctccaggag ttcgcggctc
    23581 gttccatcaa cctgacgctg ctgcacagcc gtccgaccaa gacgtcgctc ggcgactact
    23641 gctttctgct ggagcttgcc ggccacgtcg ccgacgaggt ggtggccgac tgcctgcgga
    23701 ccctgcgggc cacgcaggcc gacgtgaagt tcctgggttc ctacccggcg gccggcgagc
    23761 ggggcggcga ggtccgcgac caggccgacg cggcccagca ggcggcgcag cgctggatgg
    23821 cggacctgcg gtcccagatc aggaccgggt cgaccgactg acggttcccg cgctcccgtc
    23881 gacggtgacg acctcgccgt cgaccaggcg ctcggtcgct cccgcgaccc cgaccacgca
    23941 cgggatccga cgctcgcggg cgacgatggc ggcgtgagag gccatgcctc cggtgtcggt
    24001 gaccacggcc gcggcccgca tgagcgccgg cagccactcc ggcgacgtgg ccggcgcgac
    24061 cagcacctcg ccgtcgagca ggtccggcgc gtcgcccggt gatcgcagca cgcgggccgg
    24121 accccgcgcc gtccccgccg atgcaccggt gccggtcagc accgcgtcgg gatcgtcccg
    24181 cgtgggcgcc cctccgctca tcgggatcga tccgcccgat cctggggtcg cggatcccgg
    24241 ggcggtggca gggcgacccg tgatcgggcg ggactgcacg atccagatcg acccgtcgga
    24301 tccgatcgtc cactcgacgt cctggggcgt gccgaagtgg tcctcgatgc tccgaccgag
    24361 ctcgttgagg gcggcgacct cggcgtggga caggaccggg gcggaggtcg ccgacgggtc
    24421 gccatctccg ctcggtcggg tcgcatccac gatgatcccg ctcgtgcggt cgacctcgaa
    24481 ccgctcgggg tccgagccac ccgacacgac cgaccccccg gcgccaacgg cctcgatcac
    24541 cagacggtcg accgcggcgc gggggtcgac ggtgaacatg acgccggacc gttgcgacgg
    24601 ggccatgcgc tggacgatca ccgccatcgc cggatccgac gcgcccgacg gctgccgcgc
    24661 catgccggcg cggtaggcga tcgagcgggt cgacaccgac gacgaccagc acaccccgac
    24721 cgcggccagg accgcgtccg ccccgaccac gtcggtcacg ctgtcgtaca tgccggcgaa
    24781 cgaggcgtcc tcggtgtcct caccgatcgc cgaggagcga acggccaccg ccggcccgat
    24841 gcctccggca tcggcgtcgc cggacacgag ggagctgtac gcggcgagca acgcggagcg
    24901 gtgtccgacc gacatctcgg cgcgtcccgc gacctcccgc agcgccgcga cccgctggtc
    24961 gaggtctccg gcaccaccga cccgttcgat gagctccgcg acgcccgcgg ccacctgctc
    25021 ggcgacgccg gccacggcca gcgcggttcg gtggacatcc acggagagga cgacgccgtc
    25081 gggaacgggg agacccgcgc cgatcagctc cccgagtcgt gccgccttgg gcccgacgac
    25141 ggacatgtcg cgtcctccga cgtcggccag atcgaggacc ggccggtcgg tcaccgcggg
    25201 ggctcagccg acggcgcgtc gagcatggcc acgagcttgc gcggagcgac ggttcggtag
    25261 gcgtcctcga cgatcgcggc gacctcgtcc caatcgacgt cgacgtcgag gcgaacgccc
    25321 aaccagccgc ggtgaccgac gtagggcgga acgaagaatc gctcgggttc ggactcgacc
    25381 atcagcgcct gcgcaccgtc gggagcggcg caccagatcg ccagacggcc gtctccgtgg
    25441 tggtcgtcgt ggaacatgac gaaggtccta cggacgaaga acgtggggct gctgtggctc
    25501 agcttctcgg tcgcttcggg caggtcgagg cagatccggc gaacagcggc gagggcggcg
    25561 tcgacgtcgg cctcggtgta gggcagctcc gaacccatcg ggaccatcct cccgcaccgg
    25621 tacgatcgac gcactcacca ccctggaggg atggcagagc ggacgaatgc gacggtcttg
    25681 aaaaccgttg ggccggcaac ggtcccgggg gttcgaatcc ccctccctcc gctgcggacc
    25741 accagtccgt cgacgttcat ccgccctcgg cgaccaggcg ctcccgctcc accgccagag
    25801 cggcacgctg ctcgtcgctg atggtcggcg tcgagatcgg cagcgagtcg atcacgtcga
    25861 cgagcacgtc ggacacgagg gtgcgcatgc gccacttgcg gtccgcgggg atcacgtacc
    25921 agggagcgtc ctctgtggcg gtggcgcgga tcgcctcctc gaacgcgacc tggtagtcgt
    25981 cccagtacgc cctctccctc acgtcaccgg cggagaactt ccagttcttc tccggcgtgt
    26041 cgatccggtc gaggaaccgc agacgctgct cctcgcgcga caggtgcagg aagaacttca
    26101 ccaccacgat cccgttgcgg accaggtgcc gctcgaaggc gaccatgtcc tcgtagcggt
    26161 gctgccacag gtgcggtccc cgggcccggg ccgggaggcg ctggttggcg atcacctcgg
    26221 ggtgcacgcg ggcgacgagc acctcctcgt agtaggaccg gttgaagatc ccgacctggc
    26281 cacgttcggg aagccgtcgg gcgtagcgcc ataggtagtt gtggtcgagc tcctcaacgc
    26341 tgggcacctt gaagctggac acgacgacgc cctgcgggtt catgccgctg agcacatgct
    26401 tgatcgtgcc gtccttgccg gccgcgtcca tcgcctggaa cacgacgagc atcccgtagg
    26461 tgtcgttggc ccacagcttc tcctgcgccg cgaccagccg ggcccgattg acctcgagct
    26521 ccgcgaccgc ctgcttcttg gtgcgcagct cgagtcccga cggcagcagg tccgagccgc
    26581 tccagtcagc tgggtggtcc gccaggtgga actcggatcc gtcgctgacc cgacagacgt
    26641 cgatggctcg cggcctgctc atcggtcctc ctcccggctc tcgcgggcgc gccatcccga
    26701 ccgatgggca ccggcgacga gcggtcctgg aacgaccgta gcggtcacgc tgctcggcac
    26761 gtccgatcgg tagtgtcggc cgatcatggt ggtccgaggg ggagcgtcgc gatgagcacc
    26821 gagctgctgc accgcatcca gttcggcttc acgatcagct tccacttcat cttcccgccc
    26881 atgtcgctcg ggctgggcct gctgctgatc gtcttcggcg tccaggccgt gcgcaccgac
    26941 gacccgaagt ggcgccagct cgccatgttc tggacgaaga tctacgggct gatcttctcg
    27001 atgggggtgg ccaccgggct cgtccaggag ttccagttcg gcaccaactg gtcggagtac
    27061 tcgcgctacg tcggcaacat cttcgggagc ctgctcgcgg cggaggggat cttcgcgttc
    27121 atgctcgagg gcggcttcct gggcctgatg ctcgccggcg gtcgacggct gggcaacaag
    27181 ctgtggctct tcgccacgac catggtggtg ctcggcgcca cgttcagcgc cacgtggatc
    27241 atcatggcca actcgtggat gcagaccccg cagggctacg agctgcggaa cgagggccgg
    27301 ggcacccagg cgttcatgag cagcttccgg gaagtcgtct tcacgccgtc gttcgggccc
    27361 cgcttctggc acacgatcgt ggcggcgtgg atggtcggct gctcgctcgt catgagcgtc
    27421 gccgcctact acctgctgcg acgacgtcac gaggacctcg ccaagaccat gatgcgggtg
    27481 gcgcttccgt tgttcacggt gctggccgtt ctgcaggtga cggtgttcgg agccaaccag
    27541 gccaccgagg tcgccaccca ccagcccgag aagctggcgg ccatcgaggg ggtgtaccag
    27601 accggcgact gcgtcccgat gacgatcttc ggacgcaccg acccgtcgac gcagaccacc
    27661 cagggcctgc agatcccgtg cctgctcagc atcctggtcg gcaagagccc gtcgaccacg
    27721 gtgcagggcc tcgacgactt cccgaccgac gactgggcga acgcatcggt cgtgttccag
    27781 acctatcacc tgatgatcaa cctggggatg gcgttcgtgg ccatcggcgg actcgggtgc
    27841 ctgctgtggg cgtggaagcg ccggctgtgg acccagcggt ggctcctgtg gatcatggtc
    27901 ggatcgatcg ggctcaccca gctggccacg ctgtcgggct ggtggaccgc cgagctcggc
    27961 cgtcagccct ggatcgtgtg gcagctgctg cgcaccgagg acgcgttcag ccccaacgtg
    28021 acgaccgcgc aggtcgccgc gagcctggcc atgttcgcgg tgctgtacgc catcgtgttc
    28081 ggcgtgttcc tggtactgct caaccggctg atccaggccg gtccgcccga ccctcccacc
    28141 gaggaggagg tccaggcgct gcccgacacg tggggtgccg cgttccagca ccggtcgcgg
    28201 gtctcggacg ggagtcggtg acgtgaacct cgagacgctg tggttcgtgc tctacgtcgg
    28261 catcatcgcc ggctacgtca tcctcgacgg gttcgacctg ggggtgggca tcctgtcgcc
    28321 gttcgtggcc cgcaccgacc gagaccaccg gatgctgctc aacgcgatcg gcccggtgtg
    28381 ggacggcaac gaggtctggc tcgtgctcgg cggcggcgcc ctgttcgcgg cgttccccgt
    28441 cgtgtacgcc tctctgttca gcggcttcta cctggcgatg atgctcgtac tgctggtgct
    28501 gatcctgcgc acgatcgcca tcgagttccg ctcccaccgc gagtcgcccc ggtggcgggc
    28561 gacgtgggac tggttcttct tcgcctcgtc gctcgggatc accctgctgt tgggtgtggc
    28621 gctcggaaac gtcatccggg gcatcccgct ggactcatcc ggtcaggtga cgttgaagct
    28681 ggtcgacctg ctgaacccct actcgttggc cgtcggggtg accgcggtgg ccatgttggg
    28741 cctgcacggc gggatcttcc tcaccctcaa ggtcgacggc gagctgcggg gacggatcga
    28801 acgtctggtg ccccggctga tggtggcgtt cttcgtgctg atgacggtgc tggtggtctg
    28861 gacgctgctg ctgcgcaccg aggtgaccgc cacctaccgc gaccgcgtct ggatcgccgc
    28921 gttcccggtg ctggcgctcg tgtccaccgt cgtggcatgg cgtctgatcc gcgccggtcg
    28981 cttcctgccg gccttcgggg cgtccgctct gacgatcgcc ctgctgatgg cgacggtcgc
    29041 cgccgggatg tacccggtga tgctgccgtc gtcgatcgac tcgagctacg acctgaccgt
    29101 gcacaactcc gcgtccgctc ccgagacgct gacggtgatg acggtcatcg cggtgatcgg
    29161 gatcccgttc gtgctgctct acaccgcggg cgtctactac ttcttccgcg gtcgggtcgt
    29221 gctcgacgac cacggctact gaccgttccc atctgagcgc tgcaccgcgc ccggccccgc
    29281 tccgacgcgc ctcgagtcca cccccttgtg tggtcgctcg gccacgaacc gatacagtga
    29341 ccggctgatc tggagaggtg ccggagtcag gtcgatcggg cctccctgct aaggaggtgt
    29401 gggggcaacc tcaccgtggg ttcgaatccc accctctccg ccacacgggc gtcggcgaca
    29461 gtgccggcgc ccgtgccgcg tccgggtgtc ggcgtgggag cgggtgctct gtcgtcgacc
    29521 ttcgaacccg atccccgggg ctgaacctgg ttcgggtgaa ggaacattga cgtgacaggt
    29581 aactctcccc aatttgttgc gttttcgtga cgacctgtcg tacaactgtc atccgttgac
    29641 caagagcggt gaccctgtcg ccggtgagta cggagtggtc ccccaccagg gaccggctgg
    29701 ctgtgacccg tacctgaccc gcagggatcc cttcttcccc tgatctcgaa cgcgccccga
    29761 ccgacggggc gcccgtttgg aggagacacc catgaacagg aagtccttga cgcgcgcgct
    29821 cgcactgctg ctcgtcgtcg gcggattcgt gctcgcgtcc tgcacccccg agcagcaggc
    29881 cgcgttccag gcccacctcg actggcagaa ggcgaacgac cgcttcgccg gcgccatcag
    29941 cgacgccggc ctggcccggc tgcgggcgtg tgagtccggt ggcaactact cggcggtcag
    30001 ccgcaacggc ctgtaccgcg gcgcctacca gttccgccgc ggcacgtgga actcggttgc
    30061 cggcaagttc tacccgcacc tgcgcggcgt ggacccggcg tccgcggcgc cgttcgacca
    30121 ggaccgcatg acccgggcac tgtgggcgac cggtggtccc cgcaactggc cggtgtgctc
    30181 ccgtcgggtc tgaacggcat cgcccgcccc gcgtccccgt ccggcgatct tgtagcccac
    30241 ggtcgctctg gtgaccgctc ccctacaaca acgccgaccc tcatcgacct tgtagcccac
    30301 acgtcgccac ggcgacccac ggctacaagg tcgtcggggt tcggggccga tgcgcctttg
    30361 gcccgacccg accggccgtc taggatctga cggttcaacc agcgctcgta gctcaattgg
    30421 atagagcatc tgactacgga tcagaaggtt gggggttcga ttccctccga gcgcgctggc
    30481 cgaaggccct gacaggaggc ggttctcgtg atcgggagcc gcctctcccg cgtccgggaa
    30541 gggtccgctt cccaccggtc cttcaacaga ccagttggga tggtccatcg cgaggtgacg
    30601 atcgatgggg tgaccgccga attggccggc ggcttcatgc agagcgggca ggcgcacctc
    30661 gcgatcacat gcaccgggtg gtgcggaagc ccaccggcat cggccgcacg tacgggccga
    30721 cgtgctccca ccacgcggtc acggcatcga gcggtcagcc cgacgggcga cgggcctcga
    30781 ggtagcgcaa cgacgcctgc cgcgattccg gatcggccac cgcggcggcg agggtgtcgg
    30841 cgtcgctggc gttgcgccgg gtgccggcga tctcctccat caccgcgttg acctgctgct
    30901 tggtggtgcg cagggcgaag cccggcttgg cccccaactc cgccgccagg tccgcgacga
    30961 cgacgtcgag ctcgccggcg ggaacgacgc ggttcacgaa ccggagggcg tgcgcctccg
    31021 ccgcgtcgaa gggtcgacag gtcagcacca gctccttggt gatcgccggg ccgagctcac
    31081 gggcaagccg ggggatccca ccccatgcga gagggatgcc caggtcgacc tctgggatcg
    31141 agaaccgcgt gtcgtcggcc gccacccgga tgtcgcagga cgcggcgagg accagtccgc
    31201 cgcccacgca gtggccgtgc agagccgcaa tcgtgagctg acggacgtcg gtcagcgcct
    31261 cggccgccac ccggccgagg tcggccgtgt cacgcaccga gatggtcggg tctccgtcgg
    31321 cgacgtcgcc gaggtcgaac ccggcggaga acgcccggcc ctcgccacgc accaccacca
    31381 ccgcgatgtc gccccggtca tccaaccagc ggcacgcccg agcgagctcg ccgagcagct
    31441 gacgtgacag cgcgttgagc ttcccgggcc ggttcagcac cagcgtggcg acccggctgt
    31501 cggtgtcgtg ctcgatgcgc agggtgtcga aggtcggcgt ggtcacggga ggctcccctc
    31561 gcgatcggtg acggtgtcga caccggtggc ggtcccggtg gcgtcgtggc ggacatcggc
    31621 gtctgactcg gcaacggacg gcgtcgatcg gccggcggcg gtcgggaccg ggcagctgac
    31681 agaggtgccg cggaaccgtc cccacccgta cagaccggcg gcgaccgcag ccgcgacgac
    31741 ccacccgacg acatctcgtc cggtccacac cgcggcggtg aacccgacga agaaggcggg
    31801 gacgaccagg cgggtcaacc cgcacacgct gatgttcacg accctgtgcc tccggtccgg
    31861 gcctgggagc tacgtgccac ggcgaggaac atcgggaagg tgccctcgtc gcgctcgagc
    31921 acaccgcagt cggacacggt cacctcgtcc agacccgcag cgacgaggcg acggaccagg
    31981 tcgtcacgct cgaagccgtg gtgcccggtg aagccgtctc cgtggaagct gccgtcctcg
    32041 gcgtcgaggt cgaccaccgc gacccggcca tccggagcca gcaggtcggc gaaccgaccg
    32101 aggacgcggt cgacgtcgtc gacgtggtgc agggtcagca ccgtgacgat caggtcgaac
    32161 cggtcggcga tcgacggaat cgagtccgcc cccagatccc agtcgacgac gcgggaccgc
    32221 ggaagccggc cgtcggcgac cttcgtcgcc atgacctcgc gcatgccggc cgaggtgtcg
    32281 gccaacaccg acggacccac ccgatcgccc aacgcctcgg tgaccaaggc agtgccggcg
    32341 ccgtactcga acacgcgggc cgagcggtcc actccggtgg cgcgcagcac cgcgtccgcg
    32401 acccgaccgg accgcgcgac cttggcgtcg tcgtcccagg tggcggcccg ctcgtcgaag
    32461 ctcgccgggc cgtggtcgtc gtggtcgggg cggtgcggat cgtggtcgtg gtgcacgtgt
    32521 tctcctcgcg gtccaccctc ccacaccacg tccgtgtgcc gctcaccgga cgcgcacccg
    32581 cgtcgacacg gcccggtcgg cccgcggttg cggtggtaga cccgccccat gaaggcagcg
    32641 gtgtactccg agaccggccc gccctcggtc ctgcgctacg aggacgtccc ggacccgagc
    32701 gtcgggctgg gcacggtcct ggtgcgaaac caggcgatca gcatcgaggg cggcgacgtg
    32761 ctgcaccgat ccggcggtga gctgggtcgg gtcccccacg tcgtcggata ccagagcgcc
    32821 ggcaccgtcg aggccgtcgg cgacggggtc acctcgatga ccgtcgggga ccgggtggtg
    32881 accgtcgggg tcgacggatc ccacgccgag ctgcgggtcg tccccgaggg gttcacatgg
    32941 cggatacccg acgcgctcag cacggacgac gcggcgacgg tgccggtccc gttcggcacc
    33001 gcgcacgatg ccctgttcga gttcggtcgt ctcaccgacg gcgagacggt gctcgtgcag
    33061 gccggcgccg gcggcgtcgg cgtcgccgcc atccaactgg cgaagcgcgc cggtgcccgg
    33121 gtgctgacga ccgcatcgag tccggcgaag ctcgcccgac tcgccgagct gggcgccgac
    33181 gccggcatcg actactccga acaggacacc gtgaccgagg tcctccgcct caccgacggt
    33241 cggggcgtcg acctcgtggt cgacaacgtc ggcggctcgg cgatgaccga caacgtccgc
    33301 tgcctggcct accgcggtcg gtgcgtgtcg gtcggcgagg tcggccggga aggcgccacg
    33361 accatcgacg tcgggatgtt gaggcccaag aacctgaccg tggtcggcta cttcatgggg
    33421 atggagatgc tgatgagccc ccggtgccac gacgtggtgg cccgcctgct cgacgacctg
    33481 gccgtcgggg agctgcacgc ggtgatcgac cgcaccttcc cgctgtcgga ggccgccgat
    33541 gcccacgcct acatcgagag ccgcgccgcg gtcggacggg tgctgctccg accgtgagga
    33601 cgacgctccc gttccggatc ggagccgggc cccggtgtag cgtccggtcc gacggtggct
    33661 ccgccagcac gggaggccac acgggggaag gatcgtccat gaccgacgcg gttggagtcg
    33721 acccgggttc gacaccggcg acggagcacg gtccaccgcc gatggacgtc cggatcatcc
    33781 cgccgatggg cctgccgttc gtcgcggtcg cggtggtgtt cgtcctgtac gccatcggat
    33841 ccaactcgat ctgggcgctg atgctcgccc acgtcgccgg cggcggcatg tggaccgccg
    33901 tcgacctcta cgtcgggctg atcctcggtc cgatcctggg tcggctgtcg ccgccggccc
    33961 gcgccgagtt ctcggcccgg ttcatgccca agatggtcct gctgatgccg acgctggtca
    34021 tgatgaccct ggcgaccggc ttccagatcg ccctgaacta cgggaacctg tacccgtcgt
    34081 cgatcaaccg accctggctc gtggcctcga tggtcgtcgt cgggatcatg gccgtggtcg
    34141 ccctcggcat cctcgaaccg gcgaacatct ctgtgctgtt cgagatgaag aaggagcagc
    34201 ccgacgtcga cgtgatcgag cacctgatga aacggttcgt ctacaccgcg ggggtgaccg
    34261 gcctcatgca ggtggcaacg ctgctgatca tgacccgggt ggcgacccag tgagcagcgt
    34321 cggtccggcc acggtgcggc cgttcgagcg atcgctgccg cccgtcaccg gactcggcat
    34381 ggcggcgctg gcctgcgccg tcgtgagcgg cgtggtcgtg gcgtcccagg tcgggatgga
    34441 cgaccctccg ctggtcgtcc ccacggtgtt cgtcgccgcc gcgctgctgc tcgagatcgc
    34501 cgccgtcgtc gtcctcgtcc gcatccgacc gttcgcgtgg gaccggttcc ggcttgtgtt
    34561 ctccgtcggc ctggccgcgt acgtgctgca gagcgcgatc atcgcgtggg cgttcgtgat
    34621 caacgacgtc ccggccgggc cgatgacgat cctgtcgctc ggactcgtgg tgttcgccac
    34681 gatcgtgccc gtgatgctgg cgttcaccgt ggcccgctac caggcggtcg atgatccgag
    34741 gtgaccggtg acacggtccg aacggacaag ggtgcgtaat tagggtcgaa ggtcacggtt
    34801 cttcgaccct ccgacatcgt tactgtcatc ggtgatgggc gtcgcaggtg catcacccgc
    34861 ctcggtcatc gagttccact cacatcgggg gccaacgcgt tcggaggatg aacgtggctg
    34921 atcacgaaga acaagcagga tccgggcagg gtgccgtcag tcgccgagcg gtcgtcgggg
    34981 cgaccgtcgg gggcatcggt gcggccacca tggtcggggt cgccgccgcg gcggcctggc
    35041 ccgacagcgg aggtggatcg ggcggttcgg ccgcccccgc tgagggcggc ggatcgggcg
    35101 ggacgaccgc cgcgtcgtcc ggggcccaaa tcgacttcgc cgccaagccg gccgacgact
    35161 gggtggcccg ggatccggtg ctgccgccgg cgccggccga acgggtgcac aacatcacga
    35221 tgacggccta cgaccaggac gtcgccgagg tcgccccggg cgtcaagcag cagatctgga
    35281 cctacgacaa ggtgatgccc ggaccgttct accggggcaa ggtcggcgac cagttcaact
    35341 tcaccctcaa gaacgagggg acgttgggtc actcggtcga cttccacgct tccaaggtgg
    35401 catggaacgt ccggatggcg accatcaacc ccggcgagag catcgagtac ccgttcgagg
    35461 ccaagcacgc cgggatctac atgtaccact gcggtacgcc gccggcgctg caccacatcg
    35521 gtgcgggcat gtacggcgcg atcatcatcg acccgccgga cctggccccg gtcgaccacg
    35581 agttcgcctt cgtccagtcc gagatctaca cgggcccgca gggcgaggtc ggcagcctgg
    35641 agaagatgca gaagtcggcg tgggacgcga tcgtcttcaa cggctacgtc aacgggtact
    35701 ccgcacgtcc gatccgggtc gagcccggtc agcggatccg ggcctgggtg ctcgacgccg
    35761 gaccgtctga gaactcctcg tggcacgtga tcggcaccgt cttcgacacg gtgttcaagg
    35821 agggcaacta cctgctgcgg cccggcgacg gacagcgcgg cggctcccag gcgctcgacc
    35881 tccagcccgc ccagggcgga ttcgtcgagt tcagcttcga cgaggtgggg ctctacccct
    35941 tcgtcaccca caagttcgcc aacgtcggca tcggcgctct gggcatcttc caggcgggtg
    36001 acgtccagcc ggccgaaacc gcatcgggac actgaggagg acgacatggc tgtcgatcag
    36061 gaaagctctg acaccaccga ttcgcccgcc ggcccgtccg gtgcggtgca ctcgggcccg
    36121 gttcccgtcc gcaagggcac gaccttcgag accctggcgg tctgcgggtt catcttcggc
    36181 ctcttcgcca tcgtggcggc gatcttcgcc gtcggactgg cggcccgggc cgcgagtgac
    36241 gccagcggtt caggcggcgg cggttcagcc ggtggaggca ccgcaggagg aggcggtgcc
    36301 gcggtcaccg aggtctccct cggggagttc ttcatcaagc cgcaggacgc caccgcggcg
    36361 gccgactcgg tcctcaaggt gaagaacgac ggcacggtgg accacgacct ggccgtcgag
    36421 ggaggccccg ccaccgagat gctcaagccc ggggacgagg gcgaactcga cctggctggg
    36481 gtggcggcgg ggacctacac ctggatctgc caggtgccgg gccacgccga ggccggcatg
    36541 aagggcaccg tcaccgtcca gtgagcatcc cgctccggtg agcacctgcg cccggtcccg
    36601 acgtcaccgc gacgtccgga ccgggcgctg acgcgtccga gggccgacgg cccgcagcga
    36661 cgtacgttgt gccgatggcc ttctccgcgt ctcccttccc tctccaggag cacctcggga
    36721 tggcgatcga gtccccgagc gccggctctg gtcgggccgt gctcgacatc ggtcccgacc
    36781 tgctcaaccc caacggcgtc gtgcacggcg cggtcctgtt cgccatggtc gacaccgcga
    36841 tgggagcggc gacgatgagc gtcctcgagg atggacaggc ctgcgcgtcg atcgaggtcc
    36901 acctccggtt cctgcgggcc gcggcggccg gctcgctgac ggcggacgtg tcggtgatca
    36961 gagcgggacg gcggatcgtg cagctcgaag gtcgggtcac cggcgccgac gacgagctga
    37021 tcgcgaccgc gtcggggtcg ttcgcagtga tcaccgggcc tgcgacctga ccgggagcta
    37081 tcatacgaac gtatgagcca gcgccgttcg tcgtcggagg actccgccac ccgtgaccga
    37141 ctgctggcgg ccaccacgaa cctgatcgca cgcgacggga tcgcggccgc tacctcccga
    37201 cgcatcaccg acgaggccgg ggtgaacctg gcggccatca cctactggtt cggctccaag
    37261 gagaacctgg tcgacgcagc cctgcgcggc caggtcgcgg ccttggtcga accggccctc
    37321 gccatcctcg agcgcgacgg cgacccggtg acccgcatgg ctgcagcggt ccggtacctg
    37381 ctgagcacct tcgccgaacg acagggtcag agcgccgcct acctgaccgc tctgctggag
    37441 gtaacccgcc actccgaccc ggcagacccg acccgagccg tcgtcgcccg ggtgcgggag
    37501 cgtctggcgg cagtgaacgc agagctcgtc gcctcgggtg cggtcccggc ctgggtcgac
    37561 cccgacgcga tggccgcgct gatcatcgcc gccggtcacg gcatcgcgct gcagagcacc
    37621 ctcgaccccg acggacccac cgtcgagtcc caggccgctc agctggccgg actgctgatc
    37681 gcggcccgac cccacgacgg acccaccgag gcctcgcgac cgagaggaga ggacagtgga
    37741 cgaaccacga accggtcgga gtgaccccac cgatgcggac aggaccgagc accgtcgcga
    37801 tccggtccgg gaccggccgt cgagcggatc ctgggtcgac gtgctcggcg tgctgctggt
    37861 gctggccggg gtcgcgctcg cggtgatccg gtggttcggc gcggatccgt tggaacgcaa
    37921 cgccgaatcg ctgctcgcgt cggtgacgtt gggtgcggtc atcgccgcgc ccggcgtgct
    37981 gtgcctcatc gcgacccgca ccgatcgcaa cgcgctgctg ctgcccgccg ccatcgtgct
    38041 ggcgctcctg agcaccctgt cgccggccac cctgcccctg ttcatcgggg cgttggccct
    38101 ggcgtggcgg tgggcccgag tcggaccggg ccacggacga ctccgcggcg cgctcgccac
    38161 cgtcgtcgtg gtcgtcggac tgctgggcgc cgtggtcctg ctgttggtgc ggaccaccga
    38221 gcggaccttc tacggcaacg gcgtcgtgca cagcaccgac ggatggcagc cgtggtcgac
    38281 gtcacttctc gtgctcggcc tggtggcgct cacggtggcg gtcgcatgtt ctctcgtgcc
    38341 ggcgggctcg tcaccgaccc ggactccgtg accgtttcga gtcgtccgac cgcactgtcc
    38401 gaccgcgttg tccgaccgca ctgtccggcc gcgctatccg gcccggaccc ctccggctga
    38461 cctctacccg gtccggcggg cgacccagac gtcctcggcc ggccatcggc cggcccagtc
    38521 cgccgagacg atctcgtacc acacgggatc ctccgcaccc accggcggtc ggagctcgtg
    38581 cagggcgtcg acggtgaatc cagaacgacc cagcaccccg acccactccc cgtgacccgg
    38641 gtggtgctcc acgccgccgc cgtcccagcg gaccgtccgc acgtcatcga ccccacggag
    38701 gagtcggtca ccggccggac ccgggtcgtc ggggacgcac aacgcgctca gcgggctgtt
    38761 ggtcatgaac accaggcgac cgccgggacg cagcagccgc gccgcctcgg gcacccagcg
    38821 ctccggctcg caccacgccc ccacaccgtg ctcgctcacg accaggtcgg ccaccgcgga
    38881 gcgcagtggg acctgctccc catcggcctc caccaacggg aactccgggc cccaccggcg
    38941 ctggcaccgc cgcgcggtct gcaactgggc cggactcagg tcgacaccga caggccgggc
    39001 gccgtggcgc gccaaccagg cggagagcgc tgcggtcccg catcccagct cgacgacgtc
    39061 gaggcccgcc acgtctccga gcagggccag atccgcttcg gggcgggcga acaggcccca
    39121 ccgcaggtcg ggttcctgcc atcgtcgttc gccgtcgcgg tcggtgaagg ccgtgttgac
    39181 cccgttccag agctcacggt tgtggctggt ctcctcggtc acgatcgcat cgtcgcggac
    39241 cgggcaccct cgcgtcggcc caccacaggc catgacgcga gacagggacc aatgtcactg
    39301 gtccgatcgg atgttgttag gcatccttga tccatgggac acaccacgac cacaccggtc
    39361 gaccccgacg ccctccccgg tcacgcgctc caggccgacc ggatgcccgg gcactggctc
    39421 ctggcccgac tcggcaagag ggtcctgcgc ccagggggcg ccgagatgac ccggaacctc
    39481 ctcggcggtc tggcgatctc caccgccgac gacgtggtcg aggtcgcacc aggcctcggc
    39541 gccaccacac gcctggtcat ggcggctcgg ccggcgagct acaccggggt ggaccgcgac
    39601 ccggacgccg tcggcctcat cggcgacatg ctcgagggcc cgaaccgtcg ggtgatccac
    39661 accagcgccc aggacaccgg cctgcccgac gccagcgcgg acgtggtctt cggcgaggcc
    39721 tacctgacca tgcagcccga ctcactcaag cacaaggtga tcgcggagct ggcccgggtg
    39781 ctgcgacccg ggggccgctt cgcgctgcac gaggtggcgt tcgcccccga cgacatcgcg
    39841 gatccggacc gggagcgggc gacctcggac ctgacgtcga cgatcaaggt gaacgtgaca
    39901 ccgatgaccg tcgccggttg ggaccagctc ctcgccgaac acggactggt cgtccgttcc
    39961 cgcttcaagg ccccgttgca cctgctcgaa ccccgacggc tcgttgccga cgagggctac
    40021 ctgggagccg cgcgcttcgt gaccaacgtg gtgcgcgact ccgaggcgcg ggtccgcgtc
    40081 cgggccatgc ggaccgccat gcgggccaac gcctcccacc tgcaggcgta cggcatcgtc
    40141 gccgaacggg tcgaccccgc aggctcggga gccggagcgt gagcggcggg cgcagggccg
    40201 ccgtcggcat cggcgccgtc gtcgtcgtgg tcgccgccgc ggtcgccttc ctgctgtggc
    40261 cccgcggcac gaccgagatc tccgaaaagg acgccgtcga ggacttccgc gagaagaaga
    40321 gcaccgacac caccgcgacg gacggccagg ggaccgggac cccgacgccc ggcgtctaca
    40381 cctactccgc cgagggccag gagcgggtca agctcggtcc gctccccgcc caggaccggc
    40441 cgctgcccgc gaccgtcacc gccgtggtca ccgacacggg accggggtgt ttccagtgga
    40501 cggtcaacct gttcgccgag cacaccgagg acacgacgtg gtgcacgaac ccgtccctgc
    40561 ggatggagga gcacgccaag caccagacca tcggagcgat ctcgccgacg gccaccatga
    40621 cctgcgaccc gaatcagttg ccggctgccg gcggcacgcc gaccgagctg ccctgcacgc
    40681 tgaagctgag cggcggccca atgtcggtca cggccacgtt caccgggacc gcgaccgcca
    40741 aggcgggcga ggcggtcgac gtgggcggga cgaaggtgga aacgaccccg atcgagatcc
    40801 gctacccggt ctccggcgac atcaacggga cctgggtgga gaccacgtgg tggagccggg
    40861 accacctgcc gatccgcgtc acccgggcgc tcgagttggc cggcacggcg agcttcaccg
    40921 agaacacgac cctgcagctg accagcctcg aaccgacgtc gtgaccccaa ctcaggccgg
    40981 cagcggccac gtggccaccg tgtcgtagcg gaccccgtcg gggtcccggc ggcttcggac
    41041 caacgccagc tcctccacgc agaacgtccc gtccacccgc gtccccaacg tcgggggcat
    41101 ccgggcgtcc cgcgcgatcc gagcgatggt gatgtggccg tggaacgcac gtcgcggcgg
    41161 cggatccccg accccacggg tcgccgccac cacgacgccg gccagggcct cgaggccgtt
    41221 caccgggacc gccaggaccc gttcgccgag cacgtcgacc gcaggtccga gcgtcgcgac
    41281 cgccggcggc aggtccacgt cggccatcgc ctcctcgacg tcgtcgggat gggcttcgcc
    41341 gaagaagcgc agcgtcacgt gccaccgctc cggcgcgacg aaccgcacgc cgcgctggtc
    41401 cttgcggcgc agcgccatga gctcggacac ggtgtcctcg ggtggccaca ccgccaggaa
    41461 aagtcggggc atgcgcccag tccagcagct cgctcgaccc ggccgctccc cggttcgagc
    41521 agatccgcag ccgtccggaa cccgctcacg gcacgacggg atcaggcgac gaaggcgcgg
    41581 agctcctcga cgtcggcccg agcctgacgt tcagccatgg cgatggcgtc gggcaccgct
    41641 gtcgggtcca tcaaccgctc gatgtcgacc ttctccggca tgcgcgagaa caccttggtg
    41701 cccgacgcct cgagcgcctc gagttcggcc accgcgtcgc ccgggctctg ggtcatcccg
    41761 ccctggctgg ggaggtcggg gtcggtgccg tcggtgagct ggacgaccac ggcccgccgt
    41821 gcgcccgcca gcaggtcgag gtgcaacccg gtgccggcca agcctccgtc catggcccgt
    41881 cgatcgagga tctgctgagg tgggaacagg ccgggcaccg cgctgctcgc cgcggcggcg
    41941 cgcgggacgc tgacaccggt gcggtcggtg agcaccagcc gttcgcccgt gtaggcgtcg
    42001 acggcggaca ggtgcaacgc cggcgacggc caacgacgga acgccagcac cgcaccgagg
    42061 ttgcggcgca tggtggcggc cgacggcgtg tccgcgttca gggcggcgtg gccgatcgcc
    42121 cggacggtgt cgggatcggc gtcggtcgcc gaccagaaca tctcctgagc ccgctcctgg
    42181 ctgggtgaga agttcgaggc cggggccagc acgccgagca acttggggag ccgggccagg
    42241 acgcgcagct cgccgtggag gcgtttgatg ttgccggcct cgagcaccga gctgacgatc
    42301 gagcccgcgg aggtccccac cacccgatcg gctccgctca ggtcgactcc ggcgccgagc
    42361 aactcgttga ggtaggtcac ctgccaggcc acgaaccaca accccccgcc gccgaggcac
    42421 agacccacgg gcaggccgtc gccgtagccg gcggacatgt cgcggggcac cgccagtccg
    42481 tccggcgagg ggtcgtcgat caccagcgag cgcgtcgggg ccatggcctg caggctaacg
    42541 gtgcgacccg tcggtcatcg gccggactcg agatccagga gtgcacgctt gcggtcgacg
    42601 ccgcctccgt agccggtgag ggatccgtcc gctccgatga cccggtggca gggcacgatg
    42661 atcgacaccg ggttgcgacc gttggcggcc ccgacggcgc ggaccgcggt cggtcgcccg
    42721 atgtgggcgg cgagctcggc gtaggaccac gtctcgccgt aggggatgtc gcacaacccc
    42781 cgccacaccg tccgctggaa ctcggttccg cgcatctcga cggccacgtc gaaggatcgg
    42841 agctcccccg cgaagtaggc gtcgagctgc tcgacgacgt cgtcgaagcc cgacgggtcg
    42901 gcccgccaac cggtccggtc cggggcgtgg gcctggtcca ccatcagcag gtaccggagg
    42961 cgatcgtcgc cggcgagggt cagcgacccg atcggggtct cgatcgtgcg ggcccgcaac
    43021 ggggtgggag cggtcatgga gatgcctcct ggacacaggt cggtcggccg gcggccgcca
    43081 gccagaggtg gtgggtcgcg atcgagcgcc acggccgcca ggcctcggac cgctcgacga
    43141 gctcggatcg ccggtccgac aggccgaggg caaccgcccc ccgcctcacg gccaggtcgg
    43201 tgacggggaa ggcgtcggga tcaccgaggg tcctcatcgc cagcacctcg acggtccacg
    43261 ggccgatgcc gggcacctcg gcgagccgtg accgggaccg ggcccggtcc gcccccggac
    43321 ccaggtcgat ccacccggca gccaacgcct cgaccagggc gagcagggtg gcccgccgcc
    43381 gcgcgggcaa cgccagggga atgccctcgg cgcgggcgcc ccgcagctgc acggccaact
    43441 ccccggccgt ggggaagcga tgggtgatgc ccgtgccgac ggtcagtcga tgcgggagcg
    43501 cgactccggt ggcggccacc agccgcgccg cgtgggtccg ggccgcggcg gtcgacacct
    43561 gctgtgccag gaggacccgc accgcggtct cggtgccgtc gacgctgcgc atggcccgca
    43621 gtccggcgtg ggcagcgacg ctcggggcca gggcgggatc cgccgccagc accgcgtccg
    43681 cggccacggg gtccgcgtcg aggtcgagca accaacgaca ccgggccacc gccgaggtca
    43741 ggtccctcag gtcctcgagg cggacctccg cggccacgtg gtcgctgcgg ggcgtcagtc
    43801 ccacgacggc cgcgccgcgc tcgagcgtca gggcacgacg gaaggtgacc acgtcgccgt
    43861 cgtgggggtc gaccaccgcc tcctccaggc ccgggaccgc ctgtcgtgcc agccgtccga
    43921 acagctccga cgcggcgaac ggcgcccgca cgggcaggcg gagcaccacc cggtcgacct
    43981 cggccggccc agcccggcgg gcgcggatcc gcagctctcc gggagtcagg tcgtagacct
    44041 cgcggacggt tgcgttgaac tgccgcacgc tcgagaagcc cgccgcgaag cagacctcgg
    44101 cgaaggccag gtccgtggtc tcgatcaggg tccgggcggt gcgggcacgc tgggcccggg
    44161 cgagtgccag cggaccggtt ccgacctcgg cgcgcaccag ccgctcgacc tgccgggcgc
    44221 tgtaaccgag tcgacccgcc aggccctcga ccccgtcgcg gtccaccacg ccatccgcga
    44281 tcagccgcat cgcccgggcc acgacatcgc tccggggatc ccactcgggc gagcccggcg
    44341 aggcgtcggg ccggcagcgc ttgcacgccc ggaacccggc gctctgagcg gccgcggcgg
    44401 tcgggtagaa gcgcatgttg gcccggcggg gggtccgggc cggacagctc ggtcggcagt
    44461 agatgcccgt cgatgtcacc gccccgacga accagccgtc gaagcgggcg tcccgggcgc
    44521 acacgacccg gtagcagcgc tcggggtcga gggcgctcgg gtcgacagcg ttccggtcga
    44581 cagctttcca gtcgatgggc acggcgtcag gctgccacga cccacgccct cgggctggcg
    44641 gaaatccgac atgcaccatc cgatgtcgtt cggaccggtc ggggcggacg acggaccggt
    44701 ccgagccgcc gtggccacga ccgaccgacg gcggcgcgag gattcgacga cagccccggg
    44761 gcgacccacg gcccccgccc ccgacgccga cgtcaggaga cccatgccga tcctccctcc
    44821 ccctccggtg ggctcgttcg tcagcgacaa cgccgccggg gtctcgcccg aggtcatgga
    44881 ggcgctcgcc gcggccaacg tcggaaccgc catggcctac ggcgacgacg actggacccg
    44941 acgcgcccag gcccgcctgc gcgaggtgtt cgacgctccg gtgcactcgt acttctgctg
    45001 gggcggcacc ggcgccaacg tcgtcggcct ggccagcgtg ctgcggccct ggcaggcggt
    45061 cctgaccgcg gcgagcgccc acatcgtcgt cgacgagtgc ggggcgcccg cccgcttcac
    45121 cggctcgacg atcgtgccga tcccgaccga cgacggccgt ctgtcgcccg acatgctcgc
    45181 gccctggctg cagtggcagg gcagcgaaca ccatccgcag cccgccgtcg tgtcgatcac
    45241 ccagtcgacc gagatgggca ccctgtactc ggtcgacgag atcggggcgc tgtgcgacgc
    45301 cgctcacgcg gcggggatgc tggtgcacct cgacggtgcc cggatcgcca acgcgctggt
    45361 ggcgaccggc tcggacctgc cgacgatggt ccgcgacacc ggcgtggacc tcatgaccct
    45421 cggtctgacc aaggacgggg ccatgttcgg cgagacggtg gtgttcgtcg ccgcggagcg
    45481 cgaccgtgca ccgctggatg ctgaggcgcg cttcgtgcgc aagcaggccg gtcagctcgt
    45541 ctccaagagc cgcttcgtcg ccgcccaggt gctcgccctg ctcgaggacg acctgtggct
    45601 ggccaacgcc cgtcacgcca acctcatggc cgcccgcctg gcccggtcgg tcgaggcgat
    45661 cccgggggtc cgactcgccg acgagccgca ggtgaactcg ctgttcgtcc acctgcccgc
    45721 tgccgcgatc gccccgctgc aggagtggtc gttcttctgg gactgggacc tgacggttga
    45781 cctggtgcgg tggatgacca gcttcgccac caccgacgag gacgtgcagc gattcgcggc
    45841 cggcgtctct gcggtgctgg ccgaccacgc ctgataacgg accagcgccg gtcggtaacc
    45901 tgaccggccg tgaaggccgg tcggaacgcc ccctgctggt gcggtagcgg cgccaagctc
    45961 aagcgctgcc acctgcaccg tgacgtcctc cgccgccctg cggtccatct cggtcgggtc
    46021 ggtgccctcc ggagcgtccc cgccgccatc gcccgtccgc cctacgcatt cgacccgccc
    46081 acgtggccgc tgcccgaacc ccagatcctg agcggagcgg cgctcgagcg catgcgccac
    46141 gccgggcggg tcgcggccga ggtcctggcc gagacggtcg cggcggtcgg agtcggggtg
    46201 accaccgacg agctcgacgc cgtcgcgcac gacgcctacg tggctcgggg tgcctacccg
    46261 tccaccctcg gctaccacgg cttccccaag tcgatctgca cgtcggtgaa cgaggtgctc
    46321 tgccacggga tccccgacga ccgacccctc gccgacggcg acatcgtcaa catcgacgtg
    46381 accgcgtacg tcgacgggat gcacggcgac acgtccgcga cggtcggggt cggcacgatc
    46441 tcccccgcgg ccgcggcgct gatcgacacc acccgcgacg cgacgctggc cggcatcgcc
    46501 gacatcgtcg ccggcgaacc gatgcagcgg atcgcggctg cgatcaccgc ggtggcgtcg
    46561 gcccgtggct acggcgtggt ggccgagtac ggaggccacg gcatcggtga ggtgttccac
    46621 gccgatccgc acgtccacca cacactcacc tcccgcgacg actcggtggc gctacccggg
    46681 ctgtgcgtca ccgtcgaacc gatgctgcgc accggggcgc cacggttcac ccaggccgac
    46741 gacggctgga ccgagatcct cgacgacggg atgccgtcgg cccagttcga gcacaccgtg
    46801 gtcgtgaccg acgacggcgc cgagatcctc accgtgctgg ccgacgggtc gagcgccgtg
    46861 tcgccgacgg gccccgcctc agcggcctga gcgactcacc gaccccggaa cgcctcgagc
    46921 agcagctcca ggctccggac cctcaccgcg gtgtcgtggg cgaccgacga caccatcagc
    46981 tcgtcggccc cggtgcgctc cgccagagcg gcgagctcgg acgcggcgcg ctcgggcggc
    47041 ccgacgatgc ggttggttcg ggcgacgcgc gccacgtcgg cccaccggtg ccggggagcc
    47101 tcgtcgggat cgaccagtcg gatgcgctcc ccggtgcgga tgccgtgcac cagcagctgg
    47161 ccgggaaggg cctgtcgctc ggcctcggcc gcggtgtcgg cgaccaggac gttggcggtg
    47221 acgatgaggt gcggcgccgt cagatccgcc gacggtcgga acgatcgtcg gtacagctcg
    47281 acggcctggt cggttccgcc catgtcgaag tggtgggcga acgcgaaccg cagcccgagc
    47341 agtcccgcca ggcgtgcgct gtagccgctc gagccgagca gcaggatctg cggacggctc
    47401 accgcccgcg gcgtcgccgc gaagcggtcc cacagcggcc ggtcccctcc gaccagctcc
    47461 ccccaggcat ccggacgcgg gtcgccgaga aggcccatca gctcgagcag gtcgcggggg
    47521 aagtcctcgg ccgacaggtc cgcgctgcgg cgcagcgcca tcgccgtgta cgggtctgtt
    47581 cccggggccc gaccgatgcc gaggtcgacc cggccgggat gcaacgcctc gatcgcagcg
    47641 atctgctccg ccacgaccag cggggcgtgg ttgggaagca tcacgccccc ggaaccgacc
    47701 cggatgcgat cggtgaccgc ggccaggtgc gccatcagca ccggcggggt cgtgctggcc
    47761 accgacggca ggttgtggtg ctcggccacc cagaaccgct ggaatccggc ccggtccgcg
    47821 gcccgcgcca cctcggtggc acccgccagc gcgtcggcgg acgtgccgtc ctcggtcagg
    47881 accgcgagct cgagcaccga cagggggacg gtggggttca cgcctgcacg ctacgacgcg
    47941 ggcccgcccg acgccgttag cttggcgggt gatgacctcg gcaccgtcga gcccgatcgc
    48001 gccaggcccc ctgatcgagg accacgcgct gatcggcgac acggtcggtg tcgccctggt
    48061 cgaccgctcg ggttcgatcg actggtggtg cccgtcgcgc gtcgatgctc cggcctgctt
    48121 cgccaagctc ctcggcaccg cggacaacgg ccactggcgc ctcgctccgc gggatcccgt
    48181 catcgaggtc cgacggcgct acgaacccga cacgctcgtg ttggagaccg agatgcgcac
    48241 cgcgtcgggg acggtcgcga tcatcgactt catggttccc gagcaggagc acccgacgat
    48301 ccaccgctgg gtcgagggtc gctccggcac ggtcagtatg ggaatggagc tggtcgtccg
    48361 cttcgactac ggggcggtgg tcccgtgggt gcgctcgacc ggcgacggac tcgacatggt
    48421 cggcgggaac gaggcgctgc ggttccacag cccggtgcct ctgcacggtc gcgacctgcg
    48481 cacctacgcc gagttcgacg tgagcgtcgg agagcagcgc tccttctcgt tggcttggca
    48541 cccgtcgacc gaccccgcac cgctcccgct gtcgaccttc gcggccctgc accgaacccg
    48601 gacgtggtgg cggacgtggg tgtcgcactg cacctacagc ggaccgtggc gccacgacgt
    48661 cgtccgctcc ctcatcaccc tgaaggctct gacgaacagc cgcaccgggg cggtcgccgc
    48721 ggcggccacc acgtcacttc cggagtggat cggcggggtc cgcaactggg actaccggta
    48781 ctcgtggctg cgcgacgcca ccttcaccct gcaggcattc ctcatcgccg gctacgtcga
    48841 cgaggcacgg gcctggtcgc ggtggctgcg tcgggccgtg gccggtcgcc ccggcgactt
    48901 ccagatcctc tacggcgtcg gcggcgagcg acgcctcccc gagctggagc tcgactggct
    48961 ggccggctac gagggctccg cgccggtgcg cgtcggcaac gccgcgtccg ggcagttcca
    49021 actcgacgtg ttcggagagg tcatggacgc cgccgccacc gcgcgacggg ccggcgtgct
    49081 gcccctcggt cgcgacggac tcgatgtggc ccgggcgctc atgacccacc tggaaggggt
    49141 ctgggaccag cccgacgagg gcatctggga ggtgcgcggc ccccgacgcc acttcaccca
    49201 ctcgaaggtg atggcctggg tggccttcga tcgggcagtg cgtctggcct ccgatgccga
    49261 ggtgggcgcc ttcgccgtcg acggccaccg acgcccaccg gacccgggac gcgtcgcccg
    49321 ttgggccgaa ctgcgcgacc gggtgcacga ccaggtctgc accaacggct ggaacgacga
    49381 catcggagcg ttcacgcagt actacggatc gaccgagctc gacgccagcc tcctgatggt
    49441 gctgccggtc gggttcctga cccccgacga cccgcgggcc gcctcccacg tcgacgcgat
    49501 cggccgggag ctcagcgtgg acggcttcgt gcgccgctac cgcaccgagc ccgacgacgc
    49561 ccactcggtc gacggcctgc cccccggaga gggggcgttc ctgctcacga cgttctggtg
    49621 ggccgacaac ctggccatgc tcggtcgagt cgacgagtcg atcgaggtgt tcgagcggtt
    49681 gcggagcctg cgcaacgacg tcgggctgct cgccgaggag tacgacccga cgtcgggacg
    49741 gatgctcggc aacttccccc aggcgttctc ccacgtcgga ctggtcaaca ccgcggccaa
    49801 catcgcggcc gcccagggtc acccggagac cgccgtcgcc ctcacccgct tcacccgctg
    49861 tccggatccg agcccgtccg gagtagaccg gacctcgtga gcacggccga cccaacgccc
    49921 ctcgagacgc ccggattccg catcgagcac gactcgctgg ggccggtacc ggtaccgatc
    49981 gaggcgcggt ggcaggccca gacccagcgg gccatcgaca acttccccat ctcgggcggg
    50041 cgggtggcgc ccggactgat ccgggcactg gtggagatca agcgcgccgc ggccctggtc
    50101 aacgtcgagg agggccacct ggaccgggtc gcagcggaag ccatcgtctc cgcggccgac
    50161 gaggtgctct ccggcctcca cgacgaccag ttccccatcg acgtgttcca gaccggctcg
    50221 ggcacctcga cgaacatgaa cgtcaacgag gtcctggcca ccctcgccac agtgcgcgcc
    50281 ggcacaccgg tgcaccccaa cgaccacgtg aacgccggcc agtcctccaa cgacacgttc
    50341 ccgtcggcca tccggatcgc ggcggcgaca cggatcgtcg gccacctgat cccgtcgctc
    50401 cagcacctgg cccggtcgct gcgacgcgcc gaggaccgct tcgcggacgt ggtgaaggcc
    50461 gggcgcacgc atctgatgga cgcggtcccg gtcaccctcg gtcaggagtt cggcggctac
    50521 gccaccgcgg tcgagtcggg cgtccggagg ctgcggtccg tgctcgttcc gttgggtgag
    50581 ctccccctcg gcggcaccgc tgtcggcacc ggcctgaacg ccgatcccgg gttcgccgcc
    50641 gcggtcatcg cccgggtcgc cgagcgactg gacctgccgt tgaccgaggc gccggaccac
    50701 ttcgaggccc agggcgccca ggacgtcctc gccgaggcca gcggggcgtg tcgggtcgtg
    50761 gcgatcggcc tgaacaagct cgccgcggac ctgcggtgga tgtcgtcggg tccggccgcg
    50821 gggctgggcg agatccacct tccggacctc cagcccggca gctcgatcat gccgggcaag
    50881 gtgaacccgg tgctgcccga ggcgacccag caggtggtgg ctcaggtgtt cggcaacgac
    50941 gccgcggtgg cgttcgcggc ctcggcgggg aacttcgagc tcaacgtgat gctgccggtc
    51001 atggcccgca acctgctcga gtcgatcgag ctgttggcca acgtgtcggt cctgctcgcc
    51061 gatcgctgca tcgacgggat caccgcggac gtggatcgag cccgccgcta cgccgagtcg
    51121 tcgtcggcca tcgtgaccgc cctggtcccg ttgatcggct acgaggctgc cgcccaggtg
    51181 gcaaaggcgg cgctggcatc gggccgcacg atccgcgagg aggtcgtcgc ccgcgggttg
    51241 gtgtcggacc ccgacgagct ggaccgggcg ctcgacgtgc tcgccatgac ccgcggcggc
    51301 cgcctcaccg accgctgacc accgttctgt agtgcgagat ccaggcgaac gcctggatct
    51361 cgcactacaa agcgggtgag ggggtgccgc tgggacgttc agccggactg acgccgaccg
    51421 aggaccttgc gtccgagccg ccggggcgac caccgaccgt gggccaggtt gatcaaccgc
    51481 cccaccgacg ggtcgaggcg cttggcccag tcgcggacct cggagctcga ggtcaggtag
    51541 aggatctggc tgcgatcggc acgggccgtc accgcctcga ggaccgcgat cgcctgcgcc
    51601 gacggcaacg gtccgaacgt gtcgcagagc accaccggcg gggggttggt cgcccaggga
    51661 tccagtcgcc gcagcacggc ctccacgacc gggacgacgt cgtcggggcc gtagcggtcc
    51721 gccggcaccg catcgagccg atcccgctcg tcggtgatcc ccgacagatc ccgggtggcg
    51781 ttcgccacgc ccgcctcggc gtcggccaac cggacagcca gcgatgccgc ctcgggcacc
    51841 gcgtcgccca gcgccacgtc gagatccgcg atgcgggcat cgatctgctc acgctgtccg
    51901 acgagctcgc gggcccgctc gccgagcgac acctcctcgt cgtccagatc cgcgatggtc
    51961 ccccgatacc gggcgacctc gtcccgggcc gtctcgatcg cgtcgtcgac ggcgagcgtg
    52021 aggcgggtga cggagtgcgg gacctcgggg atgcggctca gcgcgtcgat cgaggatccc
    52081 tcgggccgga accccaggag agccgtgacc ttctgctgag cgggctcacg ctccgcggcg
    52141 agctgctcga tgcgctcggc gacccgggac cgcgccgcag ccagttcgcg atcgaggctc
    52201 tcgatgtgct cgtcgagatc ggcgagcttg gcctcgaccg cttggccgac gctgcgagtg
    52261 gacgtggcga tcgtgtactc caggtaggag tcgaacccgt agcgcgccag aacgacctgc
    52321 tccgcggcgg ccgcatcggc ctcctgggcc ttggacgcct ggagcagcgc gatgtgggag
    52381 gcgtcgatct gtgacttggc ggtgtcgccg agcatccccg acgcggccag gccccgcagc
    52441 agttggtggt tggtgtcgag gtcggccctc tcgctcgcca gtgcgacaac ctcggggtcg
    52501 ccctccaccc cggttcgcgc cgccagctgc caggcccgct ccaccgcatc gacctcggcc
    52561 agcagagcct gggcatcggc tcgcaccggc acctgggatc gggcgacgtc acggagccac
    52621 gtccggacct cgtccatctg ctgagcggac agccctccga gcgcgccggc ccgttccacc
    52681 aacgccacga gctcacccgt gtcgtcaccc atgcggatgt cggccccgtc ctcaccgggt
    52741 ccgagctcgg tcaccagcgc ggcacgcaca ccgcacagct cctcgaggcg atccgcggcc
    52801 acgtccgccg cgtgcccggc ggagtcgcgc tccgtcgcca gcgccggacg ccgggcgtcg
    52861 agcgcgccgg cggacacgtc caggacttca cgacgagcga cgcactcctc gcgctcctcg
    52921 gttccggccg ccaccgcggc ctcggtcgcg gcctggagcc gggcgatccg atcccgttct
    52981 tcgacgagct cgcccaacgc cgcgttcgcg gcatcgatcc gggcgtcgag gaccgctcgg
    53041 gcacgttgac gcgccgtcgg atccggcgcc ggcagatcgg ccgggccgat ccgatcgaga
    53101 ccctctccga tcaggtcgag agcgaccacc gcggtcggat cgaggggcgt gaggtagtca
    53161 ccgccgtcga cggtgccggt gacctcggtg ccggcgagga cgtaggcccg cccgagcagc
    53221 gccgccaccc tctcctggag cgcggctgac gcgatcacca cgttgagcct gggatgcagt
    53281 tccagatgga cgccgttgtc ggtgcccacc tgcaggtcca ccaatcgcac ggacccacct
    53341 ccttctgcgc ccccatcggc acggaccaga ggaagtgaag tgaaccgctg tcggaatcag
    53401 ctcgctcggt cccgggtgtc gcgcaggagg acctcctggg cgaccgtggc gacgtgcgtc
    53461 ccgtcgaggg cgaagatctg tcccaacgac aggccccgcc caccgacgaa ggtgtggcag
    53521 gtgaagtcgt ggaggtgcca ccggtccgcg gcgagcgggc ggtggaacca gatcgtgtgg
    53581 tccaggctcg cggtgaagag cgccttgtag agcgtctcgt cgggctcgct gcgcaccgga
    53641 tgggcctgca cgactgcgtc ggtgggcagg tcgtcgctca tgtaggccaa cgcggcggcc
    53701 tgacggaacc ggtccccacc gatcgcctcg gtggtgcgca accacgcggt ggcccggccc
    53761 cggccgtcgc gctcggcggc cgggctcacc cgatccccgg ggacgaacgc ccgctcgaac
    53821 gccgcgctcc atgagctcgg ctccagctgg tccggcgtcg gcagctccgg cggcggggcc
    53881 agggtctcga cgtcggccga tccctcgtcg cgctggaagc tggcctccag gttgaggatc
    53941 gctccgacgg cctgacgggc gacgacacgg cgggtggtga acgagcgccc gtcgcggatc
    54001 ctgtcgacct cgtagcgcac cggttcgtcg tggtcgcccc gccggatgaa gtaggcccgc
    54061 agagagtgga ccggcatctc gtccgacacg gtgtgggcgg cggccatcag cgcctgggcg
    54121 acgatctggc cgccgtagag gccgccccac gggtagcggg ggccgatccc gacgtagacg
    54181 tcgcgtccgt ggctctcgag gtcgaggatg ctctgcaggt ccacggtcgg cgacgctagt
    54241 cgccagctga cccaccgctg ccggccggac gaggggcgtc gtccggccgg ctcgcggctt
    54301 cgggggcgag ttccgcagcg gaggtcgtcc gcacggagcc gtcccggatg gcatcatcgc
    54361 cggtgccgtc ccgtgccgaa atggggaggg ctccccggcg accgggccgt tcggcccggt
    54421 cacgtgcacg gcccgagacc ggagcgaacc tgatgaccga accgaacgcg tcgaccgcga
    54481 tcccgccggt ggacgagaag ctcctggagc aggcggtggc gtggacccgc gccgcgggcg
    54541 agctcaccct cgggtggttc aaccaccccg agctcggggt ggagcacaag cacgacgggt
    54601 cgcccgtcac cgccgcggac cgcgccgcgg agcgcctgct ccgggaagag atcgccgccg
    54661 ctctccccga cgacaccgtc cggggcgagg aagagggcga tcgcgtcggc acctccggcc
    54721 gcacctgggt catcgacccc atcgacggga cgttggcgtt cagtcggggc gtcggcacct
    54781 actccaacct cctgtacatg gaggacgagc acggtccggc catcggcgtg atcaacctgc
    54841 ccgccctggg cgagacggtc tgggcgggac gcggtcgagg gtgcttcctc gacggtgccc
    54901 gctgctcggt gtcggatcgc gccgagctgt ccggctcgta cctgagcgtc accggcttcc
    54961 acgagtggac cccggacatg ttcgaccggg tgaccgggtc aggcctgcaa ctgcggacct
    55021 ggggcgacgc gtacggctac gccctggtgg cgtccggacg ggtggaggcc atgttcgacc
    55081 ccgccctggc ctggtgggac ctggccgcga tgctggtcgt catccccgag gcgggcggca
    55141 ccctcacgtc gtggtcgggg gaggatccgg cggtggagaa catcgaccat cccgagtacc
    55201 gctggtcggc gatcgcgtcc aacggtgcct tccacgaccg cctggtcgag gtgctcgccc
    55261 gctgagggtc agccgcccgc accgaccagg gactggacca ggtcgacgaa cgcggtgtcg
    55321 gactcgggat ggtcacgctc ggtgatgcgg tccgtcggag cggtggcggc accgatcgca
    55381 tccacgtagc gggcccggcg acgtgcgacg agcccgtcgg gagcttcgcg gtcctcgacc
    55441 ggcacgtact gcgcctcgat gcccatcttc agcatgccca acgcctcggt gcgcatctgc
    55501 gacacgcgcg actcggtcac acccaggaag cgtgccagct cctcggaggt gcgatcctcg
    55561 atgaagtagc cgagcacgat gacccggtgc cgctcgggca gcagggcgat ggcgtcgcgc
    55621 aggtaggcgt gcagctcccg ctgctcgagc tcctcggccg gctcccgctc gtcgggatcg
    55681 gccagcacgt cgatgagggt cagctcctcg tcctcggtgt caccgaccag gtgctcgaag
    55741 gccagcacga ccgagcggaa gatgcggtcg cggagtcgat cgagctcgtc gcgggacatg
    55801 ccgagcgcct cggcggtctc accgatggag gggatgcgac cgagctgtgc agccaggcgc
    55861 tgctcggtgg cgtccaggcg acgggccagg gtccgcaccg agcggggagc ccagtccgcg
    55921 gagcggacgg cgtcgatgat cgcgccccgg atgcgctggg cggcgaaccg gttgaacggg
    55981 actccgcggg cctcgtcgta gcgccgcgcc gcctcgacca gtccgagggc accggcccgg
    56041 gccagctcgt cgcggtcgac gtggcggggg aagtgcaccg cgacctggaa cacgacgtgg
    56101 ttgaccaggt ccaggtggtc ctcgaccagc cgggaggcgt cctggctgct cgccgaacgc
    56161 cggttcggga ccagggtccg ctcggtgtcc cgggtcggtc ggtcctcggt aagtgctgcg
    56221 cccatgtcgc cgtctcgctc ctggtggtct tccgggtgtc tccccgggac cagccggatc
    56281 ccggggactg cccgaggggt atcggcgcgc ggacgcgccg gacttgagca gaaatccgaa
    56341 gaaatcttcg ccccagtccg acgtcgactc gattgtcact gtccgtggtc gtatcgacgg
    56401 ttgggggccg gaactgaaca ccgaaagcga ggaaattcgg tcacaccgat cctggacgac
    56461 ggggtcgggg tgcgagtccg gatgacgcag gtgggctcag gccggatcga acgccgcggc
    56521 gcgcacgccc cgacgcgcga gctcggccag aaccggacgg ggatcgaccg acgcggcgac
    56581 cccgtggctc ccgggagcga catcacccgc cgcggcgcgc agcgcgagga ccgccgccaa
    56641 ggctgccgcg gcgacgcccg gacggtcgaa cacgccgtag accaccatct cccgacgacc
    56701 gtcacgggta ccacgaagct ccacgcgcgc cgctcccggg cccccttcga ccggagggcc
    56761 gaccagcacc ggcaggggcg ccaggacgcg gtcccggggc gaggcggcca gacgggccga
    56821 caggcgggtg acgtcgggga aggcgtcgag gaggagcagc ggttcggcca gatcggcccg
    56881 atagcagtca cgggcgctca ccgggtcggg gaaccagagc agctcgcgac ccgagaaacc
    56941 ggcgcgccgg acccaggtgc cgtccctcca ctcctgggcc gcaccccgca acgcccgtag
    57001 ccgttggcgg gcgcacgccg gtccggccgc cccgtcacgg gccacgtgga tctcgtccac
    57061 ggtgtcgagc aggtccgcgg cgtggcgagc cagcaggcac gtcagacccg gagagagacc
    57121 gacccccacg atgacgctgc ggcccgcggc caccgctcgg gggtgcaacc gcaggatcgc
    57181 ctccacctcc tcgggatcgt cggtggtggc gacaacgtgg ctgccggcgt cgagggcggc
    57241 ggacacgacc tcgagctgct cgcggggttc ccgggcgacc aggacgacgt cggccgggcc
    57301 acggctgtcg gacccgtcga cccgatcggt ctgccacagg accggcaccg acgccccgaa
    57361 cgtggcgcgc agctggtcgc gtcgctcctc gcggcgagtc gagatcgaga cgtgcgaggc
    57421 gccgggccgt ccgtccgctc ccggcgagat cagctctcgc gcggcgcggg ccccgacgat
    57481 gccggcgccc acgacggcca cccggggccg gaccggctcg gacgcgtcgg gtccactcaa
    57541 cgcaggtcgt ccccggtcag ctcccgccag ccgccaccct gcaggggcgg ctcggtggag
    57601 ccacggaagc gcagcaccag gcccaccagt ccgccgatgg ccaccgccgc cgcggctcgt
    57661 cgcagaccgc gcactgtgcc tcctcgcagg gtcgcatgca ggacccgggt ccctcgggga
    57721 cccgacggga ccgccgggat ggtggggcta gctggaatcg aaccagcgac ctcaccctta
    57781 tcaggggtgc gctctaaccg actgagctat agccccggaa cggcccggac cggatggctg
    57841 accacccgag ccggccggtc acagtacccg cgccggtcga cggcgcgcac ctcgacccgg
    57901 cggtgacccg atcgacggct cgccggggga cgctcccggt tcaggaaggg cccgactcct
    57961 gcaccggccg ggcggtctcc tcttcgatca ccgagaccca caccccgccg atgatgtcgc
    58021 tgatcaggtt gaacaccagc gtcgccacca cgatcgcggc ggccagaccc aggtggaaca
    58081 gcagcgagat cagaccgaac tgacggaaca ggaactcgcc gtcgagcttc cagtcctgca
    58141 gctgcagcga cgagttgacg aacttctcca ggctcgtgac cgtgccggag ctccgggcga
    58201 cggtccacag gatcaccacg gcgatcatcg agatgagcca catgcacagg cagaccagca
    58261 cggccagctt cagcatcgac caggggtcga cgtggcgcac cagacggcgg accttcttgg
    58321 cctcgaagcg ctggcgccga tagccgagca ccctgttggg ggtcgggtcg gactccactt
    58381 cgacggcccg tcgacgagcg gggggtccgc tcggcggtga cgggacggca cgggtcgtgc
    58441 cgacggggtc ggccggacgg gtgtcgctca cgggcgccac cctatcccga gcggtacggc
    58501 gcatcggtga gccgggtcgg ggtcaccggg cggacccggc gggccgggcc gctgcggtgg
    58561 ccacgacccc gtcgaggtcg atcgtcacca ctcgggccgc accggtgcgt cggtgcccgg
    58621 tcacgacggc accgttggcg cgggcgaccg ccgtcgccgc atcgagatca cccgtcacgt
    58681 gggccagggc gctcaggtcc gccgccgcgt cggcacgggc gcgccgggcc gcggcatcgc
    58741 cgaccctcag cacccccacc gcacacacga ccgcgaccgc cacgcccgcc gccaggagcg
    58801 gggacgccgc accccgttca gtccgggtcg acgccgcccg aatcggtccc ggcctcggtc
    58861 ccggcctcgg tcccggtcgc gtcgtcagag cccccgtcgt cggagccatc gtcgtcctcc
    58921 tcgccgccca gcacccgggc caccgccgcg acgcgggtgt cgtcgtcgag gttcatgacc
    58981 cggaccccgg tcgcgtcccg gccctgcacc gagatcgtgt cgaccgcggt gcggatgacc
    59041 gtgcccgagt cgttgatgag gaagatctgg tcgtcgtcgg ccaccagcag gccggtgatg
    59101 acacgcccac ggtcggcgtg cagcttgtgg gaacgcaccc cctggccgcc gcggccctgg
    59161 gtgttgaact ggtccacgtc ggtgcgcttg ccgtagcccg cgtcggtgat ggtcagcagg
    59221 cggacgccct cggcgatcgc cccggcgccg accaccctgt cgcccggtcg cagcttcatg
    59281 ccccgaacgc cgctggcggt gcggcccatc ggccgcacgt cggtctcgga gaagcggatg
    59341 ccctggccgc cctcggagac gacgaggatc tggtcgtcgt cggaggtcgg cagcacccgg
    59401 accaactcgt cgccgtcccg caggttgatg gcgatcaacc cgtcctgacg ggagctgtcg
    59461 taggcgttga acagcgtctt cttgaccacg ccgttggcgg tgacgaagaa caggtaccgg
    59521 tgcgactcgt agtcgcgggt gtcgatgatc gtctggatct tctcgtcggg ctgcaggcgc
    59581 agcaggttga cgatggccag gccctgggcg gtgcggtccc gttgggggat ccgatgcgcc
    59641 ttgaggcggt acaccttgcc ccggttggag aagaacagca ggaacgcgtg cgccgaggtg
    59701 tggatgaggt cggtgatgta gtcctcgtcc ttgagcttgg cgcccgccac gccccggccg
    59761 ccgcgggcct gggtccggaa gacgtccgcg gcgacggtct tgatgtagcc ccgcgccgtc
    59821 atcaccacga cgacctcctc gtcgtcgatc aggtcctcga tctcgaggtc gccgtcgtcg
    59881 tgctcgagcg aggtgcgccg ctcggtggcg aacttgtcgc ggatggcgac gagctcctcg
    59941 cggatcaccg agcggagccg accgtcgtcg gagaggatcg cctggagctc ggcgatggtg
    60001 gcccgcagct gcgccatctc ctcctccaag cgctcacggc cgaggcgggt gagcgtggac
    60061 aggcgcatgt cgacgatctc ggtggcctgg atctcgctga aggagaaggc gtcgcccatc
    60121 agcgccgcga tggccgacgc ccggtcggcg gaggcgcgga tggcggcgat gatctcgtcg
    60181 atgaggtcga gggctcgcag gaatccctcg acgatgtggg cccggcgctc ggccttggcc
    60241 agtcggaacc gggagcgccg ggtgatgacg tcgacctggt gctcgatgta gtgcgcgatg
    60301 gcggcgcgca ggtcgagggt gcggggcacg ccgtcgacca acgccacgaa gttggtggcg
    60361 aaggtggtct gcagcggcgt gtgcttgtag aggttgttga gcaggacgtt ggcggccacg
    60421 tccttcttga gctcgatgac gagtcggggc cgacccttgg agctctcgtt gcgcagctcc
    60481 cggacgccct cgatgacccg ggcgttggcc agctcggcga tcttctcctc gatcgactcg
    60541 accgaggtct ggtacgggat ctcggtcacg acgatgcgct ccccacgggg gccctcgtcg
    60601 atctcggccc gggcccgcat cttgatcgag ccgcgcccgg tgcgggccgc ggagacgatg
    60661 ccggcacggc ccatgatctg ggcgccggtc gggaagtcgg ggcccttgac gaactccatg
    60721 aggtcgtcgc aggtggcctc ggggttctcg agcaggtgca cggtggcgtc gatcacctcg
    60781 cccaggttgt ggggcgggat gttggtggcc atccccaccg cgatgccctg gctgccgttg
    60841 accagcaggt tggggaaccg gctgggcagg accgtcggct cctcggtcga cccgtcgaag
    60901 ttcggaacga agtcgacggt gtcctcgtcg atgtcggcca gcatgtgcat ggccagctcg
    60961 gtcagccggc actccgtgta gcggtacgcg gcgggcggat ccgacggcga gccgaagttg
    61021 ccgtgcgggt cgatcagcgg gtggcgcagc gagaagtcct gacccattcg gaccagggcg
    61081 tcgtacaccg actggtcgcc gtgcgggtgg tacttgccga gcacgtcgcc gaccacggtc
    61141 gcgcacttct tgtggttgcg gtccggacgc agaccggcct cgtgcatggc ccacaggatc
    61201 cggcggtgca ccggcttgag tccgtcccgg gcgtcgggca gggcccgcga gacgatgacg
    61261 gacatggcgt agtccaggaa ggactgctcc atctcctgtt ggatctcgat cggttcgatg
    61321 gaaccgaagg tcaccgcgac cgcgtcctca ccgggttggg aaccggcggg gtcgccgtcg
    61381 gtcggtgggg tggggtcctg cggggtgtcg tcgctcatgg aagccttggt gggtcagatg
    61441 tcgaggaagc ggacgtcgtt ggcgttggtc tggatgaagt gcttgcggct ctccacgtcg
    61501 tcgcccatca ggatcgagaa gacctcgtcc gcgatgacgg cgtgctcgac cgacacctgc
    61561 agcagggtgc gctgggcggg gtccatggtc gtggcccaca gctcgtccgc gtccatctcg
    61621 cccaggccct tcaggcgctg gaactcgttg cggtggttgg ggtggtcggc caggaacgcg
    61681 gccttggccg cgtcgtcctt caggtagatc ttgttgcgac cgacctcggt cgagtacagc
    61741 ggcggctggg cgatgtagat gtgcccgccc tcgatcagcg gacgcatctg gcggaagaag
    61801 aacgtgagca gcagcgtccg gatgtggctg ccgtcgacat cggcgtcggc catcaggatc
    61861 accttgtggt agcggatcct gtcgtagtcg aactcgtcgc ccacgccgcc gccgatggcg
    61921 gagatcaggg cctgcacctc gttgttcttg agcatcttgt cgagacgggc gcgctcgacg
    61981 ttgaggatct tgccgcggat ggggaggatc gccattgtgg cggggttgcg ggcgtccttg
    62041 gccgacccgc ccgccgagtt gccctccacg atgtacagct cggactcgcg tgggtccttg
    62101 gacgagcagt cggcgagctt gcccggcagg ccggcgccgt cgagggccga cttgcggcgg
    62161 gtcgcctcgc gggcgttccg ggccgcctca cgggcccggg ccgcctgcac cgacttctgg
    62221 aggatccgct tggcctccga ggggtgctcc tcgagccact cggagagctt gtcgttggtg
    62281 gcccgttcga ccagcgaccg catggggacg ttgccgagct tggccttggt ctgcccctcg
    62341 aactgcgggt cggtgagctt gaccgagatg atggcggtca ggccctcgcg gatgtcctcg
    62401 ccctggaggt tggactcctt gtccttcagg aagccgtgct cgcgggcgta gcggttgacg
    62461 gtgttggtga gggatttctt gaagccctcc tcgtgcatgc cgccctcgat ggtcgagatc
    62521 ccgttggcga agctgtgcag gccgtcggag ttgtagccgg tgttccactg gaacgcgacc
    62581 tccacctcca tcgagccgtc gtcgtcggac tcgtcctggc ggaagtagcc cacgtcggtg
    62641 aacagcgcct ccttggcggc gttgacgtgg cggacgaagt cgcggatgcc gccctcgtag
    62701 tggaactcga ccggctcgcc gctgtggtcg ggccgctcgt cgcggaaggt gatgcggagg
    62761 ccggcgttga ggaacgccat catctggaac cgctcggtca gcgtggcggc ccggaacgtg
    62821 atgtcgtcga agatggacgg gtccggccag aaccggacgg tggtgccggt gcggggttcc
    62881 ccgccggcac cggtcggcga gtcacccgtc tcgtgcagac ggtcgaccag atcgcccccg
    62941 tcggcgaagg ccatggcgtg gtgccggccg ttcttgtcga tctcgacctc gacgcgggtc
    63001 gacagcgcgt tcacgaccga gatcccgacg ccgtgcagcc cgccggacac cttgtagccc
    63061 ccgccgccga acttcccgcc ggcgtgcaga acggtgagca cgacctccgc cgcggacatg
    63121 tccgggaact ggaccgaggt ccccaccggg atgccgcgac cgtcgtcggt cacctcgcaa
    63181 ccgccgtcgg ccagcagccg cacgtcgatg cgggtcgcga agccggccat ggcctcgtcg
    63241 acggagttgt cgaccacctc gtacaccatg tggtgcagtc cggcggggcc tgtggacccg
    63301 atgtacatgc cgggccgctt gcgcacagcc tccagcccct cgaggacctg gatcgattcg
    63361 gcgtcgtacg tggtgggttc tgcggacaag cccgggcctc cgagggtggg tcgctggtcg
    63421 ccacgggctc gggtccgtct cctggagctg gtccgccagc tcatcccgag ggctgtggac
    63481 aacgtgtgga gaacggggtg acgagggccg aggaacggac cttcagagta ccactccccc
    63541 ctggggtcac ccggccaccg gcaccgggga ccgcgacccg cggtccggcc gaggaaccgt
    63601 gtgctcagcg acgcggatcc acccggatct cgacgcgttc gaccacctcg aacccgcaca
    63661 gctcgttggc cgcggcggcc aggtccgagc tctgccatcg gaggtgctcg gcaaccgccg
    63721 ggtcgtcgac ggccacggtg aggcagccgt cgcgcagcga gtgcaggcgg catgccgcgg
    63781 ccagacggga cccgaccagc tggggccacg actcggtgag cagcgcgagg gtgtcggggc
    63841 gcgccagccc gagcgaccgc tgcagccgtt ccagggagcc ggccagtggg tgcagggtgt
    63901 cgcggtcccg gggcagcggt tcgatcgcca cggtcccgac cctaccgagg acgcaccgct
    63961 ccgtcggcca cgtcgaggat gagctcgctg tcgaccccgt cggggagtcc cgctgcggag
    64021 gacaggacgg tctgaccttc ggggagcgat cgaagcaggg cagcgctgcg ctccgggtcg
    64081 agctcggaga acacgtcgtc gaggagcagg accggtgggc tgtcgtgggt ctcgagcagc
    64141 acccggtgcg acgccaggcg gagggcgagg gcaagggagc gttgctcgcc ctgggacgcg
    64201 tgggtccgcg ccgggagcga ccgcaacgtc agctcgaggt cgtcgcggtg cggtccgacc
    64261 agggtgactc cccgccgcag ctcgtcggag cgggacgcgg ccagagcagc cgcgagtcct
    64321 ccgggtcgat cacctgtccc ggtgctccac ggcgcgaggt accgcaacga cacatcgagc
    64381 tcctcggccg ccacgtcccg gtaggcccgg gagacgaccg gtgcgatgca ctgcaccagg
    64441 tcgtggcgca ggccggccag ggcgtcgccg gcggtcgaca gcttctggtc ccagacatcc
    64501 agggtgatca gcgccgattc gtcgggccgg ccgtgcatct gcttgagcag ggcgttgcgc
    64561 tgcttcagcg cccgttccca ctcggcgcgc acctggtcgt ggcgcgggtg cacagacacg
    64621 agcaggtcat cgaggaaacc ccgacgcaac gccggaccac ccttgaccag gtcgaggtcg
    64681 tcgggtgcga acaccgtgac ccgcaggacc tcggtcaggt cccgctggcg ggtcagcctc
    64741 tgcttgtcga ccagcacccg attgcggccg gtccgtgcga tctcggcttc gaccaggtgc
    64801 tctcgtccgt cggtcacgac ctggccgcgc accacggccc gctccgcgcc gacccgcacc
    64861 atcgcctcgg cgggcgcgcc ccggaacgat cgcagggtgg ccaggtacga catcgcctcg
    64921 agcaggttcg acttgccctg gccgttggga cccagcaccg cggtgagccc gccggggagc
    64981 tcgacgtcgg cgtcgacgta tgaccggaac ccgttcagcc agagccgctg gatctgcacg
    65041 tcggggttcc gggtgagccg ccgaagccgc tcaggacacc cggaccggca tcagcaggta
    65101 gaggaagtcc gactcgtcgg tggtgcgcag caccgccggg cgttgcgcgt cgcgggtctg
    65161 gaggacgacc tcgtcgccgg gggtgacctc gaggccgtcc atcaggtact cggggttgaa
    65221 cgcgacggtg agctcggtgc cctcgtactt ggcgtcgagc tgctcgaccg cttcgccgac
    65281 gtcctgggtg atcgcacgga gctccagccc gtcgggtccc atgctcaagc ggatcgggct
    65341 gttctcgcgg gccatcagcc gcacgcgacg gatcgcgtcg gccagggcct gccgctcgac
    65401 ggtcagcgcg ttgggcatgt cggtggggat gagcttgcgg tagtcgggga agtcgccctt
    65461 gatcaggcgg gtgctcagct ggaggtcgcc cacggtgaag ctcaccatct ggtccgacag
    65521 gacgagcgtc acgtcgtcgt cctcgccgag cgcccgggtc agctcctgca gcgagcgtga
    65581 cggcaccagg acctgttggc cctgttcgag caacgaggtg cccggcaggt cgcggtaggc
    65641 cagccggtac gagtcggtcg acacgaggcg gagcccaccg tcctcggccg ccaggagcac
    65701 accggtgagg atcggacggg actcgtcgga gctggcagcg ccgaccacct ggcgcaggcc
    65761 gtcggccagg tgcccggact gcagcgtgac cgactcgccc tcgggctcgc gggggttcgg
    65821 gtactcgtcg gccgggatcg cgttgaggaa gaactccgag cgaccggcac ggatgctggg
    65881 acgatcgccg gaggtgtcga cctcgacgcc gccgggctcg agcgctcgca cgatgtccac
    65941 cgccagcttg gccggcagca ccgcgacacc gtcctcggct ccggcgaccg acagcgaccg
    66001 ggtgatggtc aggtccaggt cgctgccggt gatgcgcaac tggtcaccgc tcaactgcag
    66061 gcgaagaccc gacaggacgg gcagggcggc tcggctcgtg gccgcacgac ctgcggtgcc
    66121 caacgcgtcg acgaggacgt ctcgttcgca gcggaacttc accgtgactc gctttcccca
    66181 ctgctgacgc cgtctcggtt ctgagaacag taggagtagt agttgggcct gtggactgtg
    66241 ggaaacctag cgcaggccct gtacctgcac ggcgggctcg cccacagcga tccagcgccg
    66301 tccccgttgc atcggtgtag ttccacgcgt gtctgtgcac agggttgcgc tgaagggtcg
    66361 gtgagcacag cgcgatccca ctcccctccc cgtcgcgatc cacctcccga cggggccgtc
    66421 agcgttcggc gagcagcgac tggatgaggg cggtgacgtc ctcgtaggtc tcgtgcttct
    66481 cctgcatgag tgccgcgacc ttctcgtatg cgtggatcac cgtcgtgtgg tcgcgaccgc
    66541 cgaactcccg gccgatctgc gggtaggaga ggtcggtgag ctcacggcag acgtacatcg
    66601 cgatctgacg ggcgtgcacc aggttgcggg tccggctgcg gctctgcagc tgctcgacgt
    66661 ccatcttgaa gtactcggcg gtctgcgtga ggatcagctc cggggtgatc tggcggggct
    66721 tggtcgactt gatcgtgtcg gcgaggatct cagcggccag gtcgacggtc agctcggtct
    66781 ggttgagcgc ggcgtacgca ccgacgcgga tcagggcgcc ctcgagctcc cggatgttgt
    66841 aggtgatgtg ctcggcgatg tagctgagga cgtcggcggg gaccgaggtg gcggcctgct
    66901 cgttcttctt ctggaggatg gcgagtctgg tctcgaacgc gggtggctgg atgtcggtga
    66961 tcagccccat cttgaagcgg ctgcggagac ggtcctcgag ggtggcgatg gcgtcgggcg
    67021 ggcggtccga ggacagcacg atctggcgtt gcgactcgta gagggtgttg aaggtgtgga
    67081 agaactcctc ctggagctgc tccttgccct ccatgaactg gatgtcgtcg acgagcagca
    67141 cgtcgatctc ccggtagcga cgcttgaagt ccggctgctg gttcttgcgg atggcgtcga
    67201 cgaactcgtt gagcagcgtc tcggtcgaga tgtagcggat ccggtacgtc gggtagttgt
    67261 cccgtacgta ctgggcgatc gcctgcagca ggtgggtctt gcccagtccc gcgccgccgt
    67321 agacgaacag cgggttgtag cgacggcccg gggtctcggc caccgacagg gcggcggcat
    67381 gtgcgaagcg gttggatgct ccgatcacga acgagtcgaa ggtgtatcgg ggcggaccga
    67441 tcggcgaggg ggcgggctcc tcggccgggg tcgcgctcag cgggcggacc gctgcgaggt
    67501 ccgggtcggt gaggtcgacg gtcagcccgt cgtcgggatc gggtcggacg tcgtcgatct
    67561 cgacctcgag gatcagtcgg acctcgggga acccggcatc ggcgacggcg gcctcgatca
    67621 tcgggaagta gcgttgctcg acgcgctgac ggacgagctg gctgggaacc accagggtca
    67681 ggacgccgtc gtggagctca tcggcgtgga tgtcctggaa ggtcgaggac cacacggtgt
    67741 cgctgacctg ttcgaccagg agcgccgccg tacgttgcca gaccgttgta ccctccgcgg
    67801 tcacgcggcc tctcctgtcg actccggtgg gcgtgctcgt gcgggaatcc ccgagcgcgc
    67861 cgtgatgatg ggtgcgatga cgtggtcggt gggtcggcgc ttgttgtcca caggcgatcc
    67921 acatctgtgg acaccggttc cggctgtcca tcgctgtgga atcggacggt agccacgctc
    67981 gtcacgcaac cgcaacccat cgtgtgaccg tcgtctcaac gatgggacgc gacgggacga
    68041 cggttggcgc cgagagccca gaacctctag cgtggtccgg tcagtcgccc gcccgcgccg
    68101 tcgtgacgtt ccgccgagga tgcacgaaga agccggcgga cccgacgatc ggcgacggtc
    68161 gagacggtgg cgcagccggt gcgcagcgtg tccgtgtcga cgtacatccc ggtcgatcct
    68221 gctgttctcg tggagtcaag gtgaagcgta cctaccagcc gaacacccgg cgtcgtgccc
    68281 gcaagcacgg cttccggcac cgcatgtcga cccgagccgg tcgggcgatc gtgaagtccc
    68341 gtcgccgccg cggtcgggcc cgcctgtcgg cctgagcgga tcccggcgga tcgtgcccgg
    68401 gtcccgtgtc ggagcacagg ggtgctggaa cggatcacac gtcggtcggt gttccgacgg
    68461 ttcgcgccgc ccgctccccg ctatcgatcc cgaacactca ccctcgtcgt tgctcctcgc
    68521 gcggagagtg agggtcatgg cgcgggtctg gccatggcga ccagtcggcg ggtcggaccg
    68581 gccgtggtcc ggaaccgcct ccgtcgtcag atccgcgccg tctcgatcga gctcgaccgt
    68641 gagagaccct ggtcccccgg gtggtacctg gtgatcgtcc acccctccgc gagaggccgg
    68701 agcagcgccg agctgggctc ggctctggcc gatgtcctgc accgggcggg agtgcggccg
    68761 tgacgcccga gtcaggcgtt cccgagacga cagcacctgt gacaacggtc acattgtcgg
    68821 gtctcaccgg accgggtagc gccgcagtcg acgggcgacc ctcggtgccg agtcgggtca
    68881 tggtccgggt ggtgcgtgtg taccagtacg ccgccgccgg tcgtccctct ccctgtcgtc
    68941 acgtccccag ctgctcgacc tatgcggtcg aggccctcga ggctcacggt gcgctgcggg
    69001 ggtcgtggtt ggcgttgcgg cggctcggac gctgccaccc gtgggggacc tccgggtacg
    69061 accccgtgcc ggagccgagg cgccgccgag ccgccgacac ccagggatcg tcgcagcggt
    69121 ccgcgatcgg gggacaggaa ccgatgaccg acggaacgac ggcggaggaa gcctgatgtt
    69181 cgagcagttc ttccaggcgc tgggcagcgt cctcaacttc ttctacacgt tgatccccaa
    69241 ctacgggttg tcgatcatgc tgctcaccgt cctggtgatg gcgttgatca ccccactcaa
    69301 cgtcaagagc actcgttcga tgctgcagat gcagcgcctc cagccggaga tgaagcggct
    69361 ccaggccaag tacaaggacg accgggagca gctcaacgcc gagctgatga agttctaccg
    69421 ggagaacaag atcaacccgc tcgggggctg tctccccctg ctggcccaga tgccggtctt
    69481 cttcatcatg tactcgctgc tccgaggtct cacggtacgt gagggtggac tcggttccgg
    69541 gatcggccac atcgccggac aggtccagca gggcgtcgag ctcacgccct gggtgttcac
    69601 agatcagtac ttccggcctg agcacctgaa ccactccacc gagctgtacc agtcgctgtc
    69661 gcacaccaac acgatgaact tcttcgggat ggacctggcc atctcggccg ccgaggcggt
    69721 gaagatcggc ctcctggtgg cgatccccta cctcttgctc ctcgtcgtga tcctgatcac
    69781 cggcgtctac cagaaccgcc agctgcaggc ccgcaacacc aacgcgacgg tcaaccccca
    69841 gcagcagatg atcatgaagg tgatgccgtt cttcctcccg gtgttctcct tcggcttccc
    69901 ctcgggtctg gcgctctact gggccaccca gaacctgtgt cggatcggca ccaactccta
    69961 catcacccgg agcgtgtacc ggaaggagca cgcaaagggc ccgatcgagg cgaccgcgtc
    70021 cgagaagacg tccacgagcg cgaagaaggg gtcgtcggga tccaaggggg gatcggcgac
    70081 cggcgggacc gaccgcggga agagcagggc cggcggtcag aagtccgcgc cgaagtcgaa
    70141 ggcgaccgtc gacccatcgg gttccgccaa gtcggcggat cgaggaccgg gaagctcggt
    70201 gaagagccag aaggcccatc agaacgcgtc ggcccccgag gggtccggtc gccgatcggg
    70261 cggaaccgcc aagcgctcgg tcaaccagcg tcgatcgggg caaccgagga gcggcggggg
    70321 atcctcgggc cgttcggcct ccgcgggaca cgtcgacgat gacgggaccg ccagcaacga
    70381 caacaccatc gagaacgaca acaccaacca gaacgacaac agggagtgag tgccaccgtg
    70441 gagtgggtcg agacaaccgg aaagacggtc gaggaggcca aggaggctgc gctcgatcgc
    70501 ttgggcgtcg acgagcagga tgccgagttc gaggtgatcg aggagccgcg gtccggcctg
    70561 ttcggccgga cacgcggcgt cggccgggtc cgtgcccgcg tcgtgccccg agccccgcgt
    70621 tccaagcctg agcggcgaaa gcggtcccgg tccgacaggg gcggtcgcgg tgagggtgag
    70681 aagcggacgg gcaccgacgc ggcgtcgtcg ggcgaggaca aggcgacggc gtcggccacc
    70741 gggggagcga agcccgctcg gaaggcatcc gcgtctgccc aggaacgatc ccagggcaag
    70801 gctgattccg gccggggtgg ttcgtcgggg cggggtccgt ccagcaagag cggcagcggt
    70861 gcccgtccgg caccgcccga caaggagcga gaggtcatgg acacggccga gcagtgcgat
    70921 gttgtcacca ccttcctcga cggcctcggc cgggcgttcg ggctggaggt gacagcgaac
    70981 gcgcagatcg aggacgacgt cctgcgggcg tcgatcgatg gcagcgaggt ggggctcttc
    71041 atcggacccg gcatggccac cctggacgcg atccaggaga tcacccgcaa cgtccttcag
    71101 cgccacgccg aaggccgcga gtacgcgaag gtcgtcgtcg acgtcgccgg ggtgcggcag
    71161 atgcgccgcg accgactggc ggccttcgtc accgacgcgg cccgacaggt gctcgacgac
    71221 gacgtcgagg tcgtgttcga ggtcatgagc agcccggacc gcaaggtggt gcacgacacg
    71281 gtcgccgaga tcgacggagt ggcgtccagc tccatcggtg agtcaccgcg acggcgcgtg
    71341 gtgctcagcg ccgccggatg acttcaggac cggagccgga cccaggtcca tcttccgaat
    71401 ccgacgacgg gcaccccgat ggggtgcccg tcccgcgttc ggggtcggtg tcggagtccc
    71461 gtgccgagca gagtcccgcg accgacgccg ccctggatcg ggtcctgatg cgctcgcggg
    71521 agctgggctt cctgggtccg gggccgaccg ccgctcacat ccaccacgcc gcagctttcc
    71581 tgcccgggat cgacgacctg gagtcccggc agggtccgga tcgggtcggt gaactcacgc
    71641 tggtggacct cggttccggt ggagggctcc ccggcctgat cctggcggtc cgtcgaccgg
    71701 gcatccatgt ggttctcatc gacgccatca ggaagcggac cgacttcctg gaatgggcgg
    71761 cggccgagct gggtgtggac gagagggtgg aggtccggcg aggtcgagcc gaggagctgg
    71821 cacgggagcc cgatctgcga ggatcagcgg aggtggtcac cgctcggtcc ttcggcccac
    71881 ccgccgtgac cgcggagtgt gccgccggct tcctgagcgg tccgggtgcg aggctcctgg
    71941 tgagcgaacc gccgacgggt tcagaggacc ggtggccggc ggagggtctg ctgcgcctgg
    72001 gcatgcagcc tggacgtctg tggtacgcgg agggcgccac ggtcatggca gtggacgtga
    72061 tcacggcatg cgccgagggc ataccccgcc gagtgggtgt tccagcccgt cgaccgctct
    72121 tctgagcgca tcgtgtcacc gggacccgcg acggctggtt ccgagacggg gtcggtcgga
    72181 caacgggacg cggcgccgac cgccggacgt tccacgtgga acagtggatc caacggacgt
    72241 cgcgatggcg gatctgacac caaccgaacg gatgaccgag tcggacatcc ggggcgacaa
    72301 gggtggtcac cccgatcatc tccgacgtgg cgacacagac ggtcctcaca tagacgctct
    72361 tcacccagac gcggtcctca cccagacggc ggcaccctgg atcggacggg accactgggg
    72421 cgttgatgtg gtcctccacg cgggtgtgaa cggcaactct gcaaccggcc ggacggcacc
    72481 gctcccgcga acgggacggc agggtcgaca ggtgtcgacc ttgccgagga tcgagaggcc
    72541 gaatcatcgg caaacgggtt gaaggtctgg cgggttgaag ctccggcgac gaccggctcc
    72601 cgcccgtagc ccgagctcac gagcaggatg tggtccccgt ctgatgcacc gctgtccggg
    72661 tctcgaccgc caactccgct ccctcgggac gtgacccgct ctgctctcac agtgcccttc
    72721 tctcacattg ccctgccctc acatcgaggt gtcgccgtga gcgggccgcg ccctcgctgc
    72781 aggcgctgct ctgtcgcgtc cggctcccgg cccgcctccg gccgacctga ctgactgggg
    72841 cgaagcgttc cacgtggaac acgcgagacg gggtcggccg gtgccggaca gcgatccgac
    72901 gaccatggtc gggcgcgagg gtgcttgacc ccggccccgc cggacggaag gatgggagcg
    72961 ctgctcgatc ggtgcgcctc gatcactcct gcgggcattc cgcgggcacg cccacgacgc
    73021 acggacccgg ccgccgcagc acctgtcgac cacctcacgt gggagttccg gatgtccgac
    73081 ctgccccccg acccgggcgt tcacgagacc gcgacctctc gcgaacccga gtggtcccga
    73141 tctgacgaac gcccgacgaa cgccgttccg gaccaccacc cagactcact cggtggcgtc
    73201 gggaacctgg aggaaccatc ctcccccgag gcgccccgcg tcgacgtcgg acccgaggga
    73261 gccggtcagg tggcatcggt ccacaacagc gtcggacctg gagccaccga tcccccggac
    73321 ccgtcccggg ccgagggtcc tgaaccgacc ggggtcgggg gtcgcccact tcccagagtc
    73381 atcgctgtcg ccaaccagaa gggcggcgtc ggcaagacga cgacgacagt gaacatgggt
    73441 gcagccctgg ccgagttgga tcatcggacc ctggtgatcg acctcgatcc tcaggggaac
    73501 gccaccacgg gtctggggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    73561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    73621 ggaccctggt gatcgacctc gatcctcagg ggaacgccac cacgggtctg gggatcaact
    73681 accggacgct cgaccacacc atgtacgacg tcctgctgca cgacgtcgct ctcgaggact
    73741 gcatcgagcc gaccgaggtc cgcaacctct tcgtcgctcc tgccagcctg gatctggcgg
    73801 gagcggagat cgagctggtc ccggccttca gtcgcgagtc gcggctgaag cgcgcggtgg
    73861 ccgacgtcat cgatgactac gacttcatcc tgatcgactg cccaccatcg ctcgggctcc
    73921 tcaccgtcaa cggcctggtc gccgcgaccg aggtgatggt ccccatccag tgcgagtact
    73981 acgcactgga gggtcttgcc cagctgaccc gcaacgtgga gctcgtccgc accaacctga
    74041 acccggagct ggagatcagc gggatcgtga tggtgatgta cgacagtcgc acacggttgg
    74101 cggagcaggt cgtctcggag gttcgagggc acttcggccc caaggtgtgt cggaacatga
    74161 tccctcgcaa catccggctg tccgaggcgc cgtcctacgg ccagccgatc acgacgttcg
    74221 atccgagctc ccggggtgcc gtggcgtacc gggagctggc ccgggaggtg agtggtggcg
    74281 cgtaagagcg gactgggacg agggctgacc tcgttgatcc cggccggagc cggggaggat
    74341 cccgatgccg tcgcggcggg gttgtccgag gtcccgatcg agtcgatccg acccaacccc
    74401 taccaacccc gccgtgcctt cgacgaggag tcgctcgagg ggctctcgga cagcatcaag
    74461 gagctgggcg tcctccagcc ggtcctggtg cgcgagctcg acgacggcta cgagctgatc
    74521 gccggagaac gtcgtctgcg ggcggccaag cgtgccggtc ttcgtacggt cccggtggtg
    74581 gtgcgaaccg ccgatgacgt ctcctccctc gagcaggcgt tggtggagaa cctccatcgg
    74641 caggatctga acccgctcga agaggcggcg gcctaccaac agctgatcga ggacttctcc
    74701 tacacccagg agcaggtggc gcagagggtc ggtcggagtc ggtccgcggt caccaacctc
    74761 ctgcgtctgt tccagcttcc gccggcagtc cagcggttgg tgggtgagca gctgatcagc
    74821 gccggtcacg ccaaggcgat cctgggtcac cccgaccgcg cctaccagga ggcgctggcc
    74881 aagcggacgg tggccgaagc cctgagcgtc cgcgacgtcg aacagctcgt ccgcgaacgg
    74941 atcgagctgg agaacagcct cgacggatca gcggcaaacg gtgctgcagc gaccgacgag
    75001 ccgacgtcgg gcgggaccgg cgcgtccgag ccgaccggcc tccggccacc ggggctgctg
    75061 gagctcgagg agctgatggc ggatcacctc gacacgaggg tgtcgatcac catgacggcc
    75121 aagcggggca aggtgctgat cgagttcgcc aacctggagg acctggaacg catctaccga
    75181 cggatcatcg attgatcgcg acgcccggga tcctggcccg gtacctcgga gtgcgaggcc
    75241 tcggagctgt cgaccgacgg tgaacctgga cgagagggtg catcgacctc gagttcaggg
    75301 tgttgtccac agggtgggca caacactggg gaaacacgaa gctgaggttc agctcaggag
    75361 gaccgggtca acctctcgcg atggttgagg tcgactgctc ccgaccggtc ggtcaggacg
    75421 ccacacccgg gtaccgggcc ggtcccggcg tctccgacgt ggcggaggga ccttcggcga
    75481 cggggtcgct ggcccagaca tccagtgccc tctgcagcga ggcgatgatc agatcgcggt
    75541 tccggtccac catcgcacgg tcgccgagtt ggaccaggac ggcggagctg cgggtctcgc
    75601 ggaggatggg aagtcgcatc ccggtgacgg cgccgatacc ccacccgggg ctggccggaa
    75661 gctccctgac cacgagctcg gccaggtgac ggcctcccgt cgacgcgaag ccgggaacct
    75721 cgaagtagct ggcctcgatg acggtggcgg gagccagcga gagtcccagg tagacgtccg
    75781 ccgcgaagtc gttggcctcg gcggcctggg ccgaccagtc gccgtcgagc agcaccgtgg
    75841 tgaccccggc ggactgcagg gccgcagcga ggcgcgtggt gaccggctgg aggtcggtgt
    75901 cggagccgat ggcgaccctc agcgatgtga gcgagcgcat ggtccgacgc aaccgatcgc
    75961 gttcgcggac cgcggtgacg gtggcccgcc cggcgcgacc ctcgagccgc atcagggcct
    76021 cgacggtatc gggcccgcag acctcgtccg cgaccagacc ggcgttgcgc tggaagtctc
    76081 cgaccgcagc cctggtcccc ggaccgaaga tgccgtcgac ccgaccggcg tcgaatccga
    76141 gcgcgccgag ccggagctgg agctcggcga cgtcgttgcc gcgcatcatc ggcgaacgca
    76201 ggcacaccaa ccgatcgccg agtcggaact ccgattcgac cagagctgcc caggtcgcgg
    76261 tgtcgcagac gccgtcgctg cgcaggccgg cctgctgctg gaacccgctg accgccgcag
    76321 cggtcgactc gccgaactcg cccgccgggt cgccggggga gtgcccggcc gcggcgagac
    76381 gccgttggag gtcggccacg gcgggcccga cggcgccgga gcgcagcgga agggcggaga
    76441 gggtgatggt gtctcccggg gtcgcggcag gtcccggtga cctgcggacc gatcgtaccc
    76501 gcagcgccgg tgcgggtcag aggtaggggt cagaggtagg gggtcagatc ctcgaggagt
    76561 tgcggcttgc cccgggcgcc gatgaggcgc tgagcctcct gcccatcttt gaaaacgatc
    76621 atggtcggga tgctcatcac attgaacttg gtggcgagtc cggggttctc gtcgacgttg
    76681 accttggcca cccgaacctt gcccgcctgt tcggtggcga tctcctcgag gatgggcgcg
    76741 accatcttgc acggaccgca ccactcggcc cagaagtcga cgagtaccgg ctcggtcgag
    76801 ctggcgacct cttcgtcgaa ggtggactcg gtgagctggc tgatggagcc catgtggctc
    76861 tcctccggtg gttcggtggg ttcgagtggt tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn
    76921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76981 nnnnnnnnnn nnnngtctca gtgctcctgg gcctcgagcc agcgctcggc gtcgatggcg
    77041 gccatgcatc cggagcccgc ggcggtgatg gcctggcggt agcggtcgtc ctggacgtca
    77101 ccgcaggcga acacgccgtc gacggcggtg cgcgacgtcc cgtccagggt gaccaggtag
    77161 ccgttgtccc tgaccgggag ctgaccggtg aacagctcgg tgttgggacg gtggccgatg
    77221 gcgacgaaca cccccgtggc ggggagatcg gtgacctcgt cggtgacgac gttgcggagg
    77281 cggacgccct cgacccggtc ctcgccgagg atctcatcga cgacgctgtc ccagacgaag
    77341 gtgatcttgt cgttggcgaa ggcccggtcc tgcatgatct tggaggcgcg cagctccttg
    77401 cggcggtgga ccacggtgac ggtccgaccg aagcgggtca ggaacatcgc ctcctccagg
    77461 gccgagtcgc cgccgccgac cacgacgatg tcctggtcgc ggaagaagaa cccgtcgcag
    77521 gtcgcgcagg tggacaggcc gtggccgagc agccggtgct ccgagtcgag ccccagcatg
    77581 agggcgctgg cgccggtggc gatgatgacg gaccgggcca ggtgcgtggg ctcgtcagcg
    77641 tcgggatcac cgacccagat ccggaacgga cgctccgaga agtccacgcg ggagaccttg
    77701 gcggtgtgga tctcggcccc gaaccgctcg gcctgctgac gcaggttgcc catcagctcc
    77761 ggtcccatga tcccgtcggg gaacccgggg aagttctcca cctcggtggt gagcatcagc
    77821 tggcccccgg gctgatcgcc ggtcgacgac ggctcgccct cgatcatcag gggttcgagg
    77881 tcggcacggg cggtgtagat ggcggcggtc aggcccgcag ggcccgatcc gatgatgacg
    77941 acgttgcgca ggtcggccat ggtggctctc cgttcggtga cggtttcggg ggtgggttcg
    78001 gggtcgatct caggtgggga gacgaccgcg cttcgaccag agcacaacac cgacgagcac
    78061 gaagatgatc ccgaacagca ggtagacggc ccaggccgga atgaagatcg tgaggagtcg
    78121 caccgtgccg acgagggcga gcaccaccgt cggcaacccc aggatgaggg cgacgatcgc
    78181 gtagaccatc cccttggaga cctcgaggat cggcccggtc gtgtgggagc gcaccttgtc
    78241 gacgttctcg acgatgagat cggtgacctg atcggtccag tcgcctccgc cgctcgccgg
    78301 ccggccgggc gtcgtggtcg atggcgttcc cgacgacgcg gaggtctcgc tcatggcgca
    78361 ggagcctagg ggttgggtcg ggtccggggc gacaggaccg tcggtcaggg gagggggaga
    78421 cacccgggga ccgacacggc ccggaccgtg tcaccggtga ggatcaccag ggccggcgaa
    78481 ccggcgacga cggcgaccgc gacgcggacc gcgtccgggc cgccggtccg acagggcagg
    78541 gtcgcgggat ccctcagcgg cagggagtcg agggtggatg tgggagtcgc cgccaccatt
    78601 gcggcggtgt ccgcgtgggt ccccaggtcc gggagctcgg ccagccgctc gtcggaaacg
    78661 acaggcgccg agcgatcggc gctgttggtc ggcgaccccg agggggcgga ctcctcgctc
    78721 gaggtcccgg cacctgacgc accagcgggg gcgggactgg tgaacggcgg cgacgtggtg
    78781 ctggtcggcg gctcgagaga gcccgaaccc tcccccgtaa cctcggtggc cgaatccgcc
    78841 aggtgctcac cggtgccgga gcggccggtg gtggcgcgca ccgcgacccc ggccgcgacc
    78901 acgaccagga ccaccgcggc ggcgatcgcc acccacgacc ggcctctcgg gacccgggtg
    78961 gatcgctccg gcctcgcgtg accggaagcg accaggtcgg gacggtgacc ggatccggac
    79021 gtggctccca tctcggccag agcggccgcc acgcgctgat cgacgtcggc aggcgacggc
    79081 ggggggtcga gcgcgagctg tcggtgccgg tcgcgcagat cctggaacct tgcgaggcgg
    79141 gcgagcagga ccggatcggc tcggacggcg gccgactcct cggcgtcggc ctctccgtcg
    79201 aggaccgcgc tcacccactc atccgcggtc ccgtcccggg gcacgcgtcg cgacccgggc
    79261 gtcgccggcg tggtctgcgg agactcggag tcgtcgggtc gtcggtcgtt catggcgggt
    79321 ccatcgcggt ggggtccggt acttcgacgt cggcaccggc cgtcgggttc ccgtcgagga
    79381 gggcggcggc tcgggcccgt ccgcgggcga tccgggagcg caccgtcccc ggggccagat
    79441 ccaggatctc tccgatgctc gcgtagtcga ggtcggcgac gtcgcgcagc accagaggaa
    79501 cccggaactc ctcggggagg cgctcgagca cctcgacgag cgtggaacgc acctcggagc
    79561 gcactgcggc atctgcgggt tcgacgtcgg tggcggcggg ctcgggaacc gaggtggcgt
    79621 cgacggtgac gggccgtcgt cgccgtcggc gcagctcgtc caggcacgag ttggtcgtca
    79681 cccggtaggc ccaggtgctg aaccgggaac gaccgtcgaa ccggtcgagc gcgcggacga
    79741 tggcgatcag ggcgtcctgg gcggcgtcct cggcgtcggc aggattccga cagagccggt
    79801 ggcacaccgc caggatccgg tcgtggtggc gacgcaggag ctggtccatg gcggcccggt
    79861 cgccgcggcg cgcggctgcg accaggtcct cgtcgctcgg cccgtcccct ccctgcgagg
    79921 ttctgcccat gtcgcgtcag ctcggtgccg gcgacccgtt ggggacgacc tcctggatct
    79981 cgacccggtg tcgggacccg tcggtcgggt cgccgaggtc ggtgatccag agcaggacgg
    80041 tggttcccgt cgccccgccg aggtcgacgc tgacggggcc acggacgtcg gtcagggtgg
    80101 ccgacgggtg caggtcgcca ggcccggcag ggtcggggtc gccgtcgagg acgtacacct
    80161 gtccgctcca tccgttggtc gagcccgaca cgcggacgtt ctccagacgg gaccggccgg
    80221 cgaggtggat gaccagaccg atcccggtct tgtcgccgaa gaagtcgggc tggtcgtagg
    80281 tctcggtccg ccacgcggtg aggtcgtcgc cgtcggtcgc ccgcgcggcc agggagtcgt
    80341 tctctcctga ggtcccgctg ccctgggggt cataggagct gaccgacgcg atgggcagcg
    80401 gctcggccgg cgcgaccgtc gaactggtcg tcggaccagc cgtgtcgggc tgacgggact
    80461 cacggaagag cagtcctgcg acgatgatcc cgaccgcgat gagcaggatc aggaaggccg
    80521 gcaggagcca tcgccgctcg gatcgtccga atccgggtgg gtcgtgctcg acggtggtca
    80581 ccgccggcgg ttcgatcggc ggacccggca cggcggccgg gacgtcgaca tcggcgtgca
    80641 gcaacgcggc ccggaagtca gcggcggagt cgaaacgacg gtccgggtcg cgttcgagtg
    80701 cccgcatgac ggcgtcagcc aggcggggcg ggatgtcgga gcggatgcgg cgggggtcgg
    80761 tcggcgtggt gtggagccgg gcgagcgcga ccgcggcgcc ggtgtcccca cggaacggaa
    80821 ctcgtccggt gaggcactcg tagaggacca gacccaggga gtagaggtcc gctcgggcat
    80881 cgacgtcggt gccggtcagc tgctccgggg ccaggtagga cgcggtgccc agcagggagc
    80941 cgtcgcgggt gagctcggtc tggtcgtcgg ccttggcgat gccgaagtcg gcgatcttga
    81001 cgcgaccgtc gtcgcagagc agcacgttgg agggcttgac gtcgcggtgc accaacccgg
    81061 ctcggtgagc gctctcgagt gcatcgagga ggcgaagtcc gatccgcaac gtggtgtcgg
    81121 gatctatcgt cccgcgctca tcgatgagct gacgcagcgt cgtggcctgg agcagctcca
    81181 tgacgatcgc ctcgtggccg tcgtcgctgc aggtgtcgta gacgccgacg atcccggggt
    81241 cgttgagtcg ggcggccgcg atcgcctcct ggcggaaccg tcggaccagc gactcgtcat
    81301 cggcgaggtg ggggtggagc accttgaccg cgacccggcg gccgaggacc gagtcggtcc
    81361 cttcccacac ctgcgccatg ccgccggcgc cgatgatgcg gtccagtcga tagcggccgc
    81421 cgagggtcgt tccggcgagg gatccggtcg gcagcgaccc ccgggtgagg tcgatcgggc
    81481 cggagccgac ctgggtctcg tccactcggc cccacgctat cggtccgtcg cggcccccgg
    81541 gccccgttcg gttccacgat gtcgacgttc ccgtcggatc gagaccgtcc accacccggc
    81601 caggaacagc agcgacagca ccgacagcgc ggcgcccacc cccgagatgg tcctcgaggt
    81661 caccgggatc gccaccgcag gaagggccag cgccccgtcg ggggaggacg cgtcgatcct
    81721 cagcagcgtg ccgccggggg cgcgcaccgt cacgggcagg tcgacccggt tctcaccggg
    81781 ccggagggtg acgatgcgtc ggtcgccgcc gccgacgtcg aggcggacgg accggatcca
    81841 cagctgcacg tccaccgcat aggggaggtc gttgcggaac cgcagcggga tcgtggcctc
    81901 gcggtcggtc agcaggacct gacgctccgg tggcatctcg atctgcgcga ccaggacgcc
    81961 gatgtcggcc aggatcctgc ggtggtaggt gtcgcggtcc gcggctgcca tgtccatgtg
    82021 cagcgtctgg gcgtcgatgc ggtcccactc ggcggcctcc gcggtcgcgg acggggccag
    82081 cgaccggtag ccgtcgatcg agcgtcgggt gtcgaggacc tcccggaggg cgggtcccgg
    82141 atcggtggga ccgtcggtgg ggacctgtgc ggtcacgggt tcgccgtcga cgacggcggc
    82201 ggcggcgggc acggcgccgg ggtcgagggt gatcggcccg gggtcggcga gcgcggcgta
    82261 gagcgcggcg agcacggccg gttcggctcc gaccggggtg cgcaggagcg tggcgggacc
    82321 ggggaactcg ctcctgccgg ctgatccgga ttcggcggcg gccggatacc acgtggccat
    82381 cagcgtcgtg acgacctggt tcgcccgttg gccgggtccg ggctcgtcgt cggacccgtt
    82441 cgccccgtcg tcgagcagtc ggctgagcga ctcatcgggg gcggtggcgg tgagggtcga
    82501 gggtgggagc ccgaccgcga gagaccgcgc gccgtcgggt gcggagcgcg ggtcgtggag
    82561 tcggagggag tcaacgggga tcgcggcgcg gcgggcgccg aggacctcga ggaacgggag
    82621 gcccgcctcg gtcaccgagt cgccgtcgta ccaggtcgtc cccaccggtt cggcgcccag
    82681 gacgcgctgg accgtcgtgt ccgtgagggc gatctggcgc agcacctcgc cgcgcccgtc
    82741 ggcggactcc gacaggctcc cgacgtcgac gtcgacgtac ggcatccggg caacggtggc
    82801 cggagtcgac ccggacgtcg ccgaccgcag gacccgcagg atgttgcggt cggccgggtc
    82861 ctcggagcgg tcgagcgcgt cgagcaggtt cggccggacg gccagggtga acggccccgg
    82921 aacgccggac tcgagcgtgc tcgccagcgc gctgagctgc tcgcgggtcg ccgggtcgat
    82981 gtcggcctgc cccgagggac gtagcgcggc gggcccgtcg acgagggcgt agaccgacaa
    83041 cgacaccgcg gcgggagagc cgtcccggga gaccggcatg gtgtcgggaa gtcggttgag
    83101 gaacacgatc gtggacgcga gctcggtgcc ggccgcgtcg gcgacgctga cggtgacggg
    83161 gtggatcccg gggttgggga ggagcacccg ttccgggtcc ccggatcggg tgccgcggat
    83221 cgggatgtcg accacgatgc ccgtggcggc gtcgccgagc gatgcgacgg tgcggtcatg
    83281 gggcgactgc aggggcggtc cggcctggtc ggtggtgatg acccgctcga cggcaccgcg
    83341 cagcgtggtg gtccgagcgg cctggagccg ctggtggatc tgggtcgtga ggacggcatc
    83401 gaccggcagg ggaccgtcca cccgcatccc gatccggaag tcgccgtcgg gatcgaccca
    83461 cggcgtcacc gcggtgacga cgatcgaggt gctcggggcc gcggcggagc cacccgcgac
    83521 ctgacggggg acggcggaca gcgacccggc gggggccacg acggcggtcg agatcgcaac
    83581 ggcgcacagg agcaggcgga tccgtcgccg gacggtgtgc tctgggaccg acctcgcgat
    83641 cacggaacgg gcgctcacgg cacgggtcgg tgcaggacga ggaggtcggt cggagtcacc
    83701 tcgaagccga ggcggcggta gagtgccagc gcgcgggtgt tgtggcgctg ggtgttgacc
    83761 agcacccgcc ggcagcggcg tcgggaggcc cagcagagcg cgtcgaggac cagggcggag
    83821 ccgatcccgt gcccggactg gtcgggatcg accgcgaggc gctgcaggaa gccctgaccc
    83881 gatccgcgac cggtgacggc gtagccgagc acccgccgcg gggcgtcgcg ctcggggagc
    83941 gccacccgga acctgcagac cggcgtggcc ttctcggcct cggtcatggc ggctcggtcg
    84001 agtcgccaga acacggggaa cgcacggttg tcgaccgcca gggcatcgtc gcggtcggac
    84061 cggcgggccc ggcggagccg gacgcccccg gtccggctcc tcagtcggtc gagcccgcgg
    84121 aggtcgtggg acaggaccct cagccggtcg tactcggtga acccggcctc caggaacggt
    84181 cgggcctcgg tcgggtgcag cgccgaggtg atcaccgagc tgtagccggc ggccgcgatc
    84241 ccgcggagac attcgttgag gccgtcgacc gacggcccgg tcggcggcgg tggcagcgcc
    84301 aggtacgcga tgcggtcgtc cgcgtgccac ggaccgaccc ggaaccggtc ctccccgacg
    84361 cgcagcagct gacggctggt catgtcctgc atcggctcca ggcggtgtcg ggtggggatg
    84421 tcggcgacga ccagacgata ggtccgctcc caaccggtgg tgggaacgcg gccccggagc
    84481 tcggtgggcg ccgacggtac gctgctcgga tgacggtgct ggtgtatgcg gacgagcgct
    84541 tcgctctgca cgacaccggt ccgttccacc cggagcgacc cgatcgcctg cacgccgtgg
    84601 ccgacggcct ggtcctcgcc ggcgtcgacg acgacgtcga gcgggtcatc gcccgccctg
    84661 ccaccgacga ggagctgttc cgggtccatc ccgaggcctt cgtccggggg gtggaggggt
    84721 tctgccggtc ggggcggacc cacctcgacg ccgacacggt cgtgtcctcg gcgtcggcgg
    84781 acctggcccg gctgggctcc ggtgccgggc tcgacgcgat cgagcgcctc cgttccggcg
    84841 atgcggacgc ggccttcgtc gcgccgcggc cgccggggca ccacgccacg gcgagccggg
    84901 ccatggggtt ctgcctgtac aaccacgtcg cggtggccgc cgcggcgctc gcggcggcgg
    84961 gggagcgggt cgcggtgatc gacgtggatg cccaccacgg caacgggacg caggacatct
    85021 tctaccggga tcccgacgtc ctctacgtca gctggcacca gcatccgctg tatccgggga
    85081 cggggagagc atcggagatc ggcgaaggtc cgggtcgggg ctccacgtgg aacctgccgg
    85141 tgcccgccgg cgccaccggt gaccattacc gccggtcgtt ggaggagctg gtcgcccccg
    85201 cggtcgaggc ccacggcacg acctggctgc tggtgtccgc gggcttcgac gctcaccgcg
    85261 acgacccgct caccgagctg ggtctgacct cgggtgacta cgcggacatc gtggccgacc
    85321 tgctgacgct ggtcgcgccc ggtcggcggg tggtgttcct ggagggcgga tacgacctgg
    85381 aggcgatcgc ccggtcgacc gcggcgaccg tctcggccct gtacggcgag cgcctgcatc
    85441 ccgaggcgcc gacgagcggt ggtcccggcg acgacgtggt ggaacgggtg acggccaccc
    85501 gtgaccgcgt cgctagcctc gggtcgtgat ccccgagcgc gcccgtgagc tgatcgacga
    85561 ggtccggccg ctggccgagc gcttcgcggc ggccgacgcc cggctgttcc tggtcggcgg
    85621 cgtcgtgcgc gacctgttcg tcgtggcggg gtcgggcacc ggtccgatcg gtgacgacgt
    85681 cgacctgacc accgacgcgg agcccgcggc gatcaaggcg atcgtggcgc cggtggccga
    85741 cgcgctgtgg acccagggcg agaagttcgg gacgatcggc gtctcgatcg gcggtcggac
    85801 ctacgagatc accacccacc gggccgaggc ctacacctcc gacagccgca agcctcaggt
    85861 ggcgtacacc accgaggtcg aggtcgacct gtcccgtcgg gacttcactg tcaacgcgat
    85921 ggcgttggag gtcacgcacc ccgaaccggt gctcatcgac ccgttcgggg gtctggccga
    85981 cctggccgcc ggtttgctgc gcaccccgct cgacgccgag gtgagcttct ccgacgaccc
    86041 gttgcgcatg ctccgggccg cccggttcat cgcccgctac ggcctggccc cggtccctga
    86101 gctggtcgcc gcggttcgcg acggtgcgcc gcggctcgag atcgtgagcg cggagcgcat
    86161 ccgcgacgag ctgtgcaagc tggtgaccgt cgatgacccg tccgccgggc tgtggttcgt
    86221 gcacgacagc ggcctggccg ccatgttcct gcccgagctt ccggcgatgc gtctggagca
    86281 ggacccgatc caccgtcaca aggacgtgtt gagccacacg atcgcggtgg tggccaaggc
    86341 ccgcaacgag ctcaaggtgc ggctggccgc gctgttccac gacgtgggca agcccaagac
    86401 ccgctcgatc ggcggcaagg gcgtgagctt ccaccaccac gaggtggtcg gggcccgcat
    86461 gacccgggac cgcatgcggg cgctgaagtt cagcaacgag atggtccggg acgtgaccca
    86521 gctcgtctac ctgcatctgc ggttccacac ctaccagatg ggctggaccg acgccgcggt
    86581 tcggcgcttc gtccgcgacg ccggatcgca gctcgacgag ctgatcgcac tcacccgggc
    86641 ggactgcacg acccgcaaca agaagaaggc cgaagcgctc gaccgtcgca tcgacgacct
    86701 cgaggaccgc attgccgcgc tgcaggcggc cgaggagctg agcgcgatcc gacccgacct
    86761 ggacggccag caggtcatgg cgcacctggg tctggcgccg ggtcccgagg tgggccgggc
    86821 gttggcgcac ctgctggagc tgcgcctgga ccgtggaccg atgaccgagg acgaggccta
    86881 cgccgccctc gacacctggc acgccaccca gatatagggg tccgcaccga cacaccttgt
    86941 cgcaatccgg acctcagaaa tccg
//
LOCUS       DAOQOD010000002        60206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_134_length_60206_cov_13.299164, whole genome shotgun sequence.
ACCESSION   DAOQOD010000002 DAOQOD010000000
VERSION     DAOQOD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 60206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60206
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_60206_cov_13.299164"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLZ72_00460"
     CDS             <1..586
                     /locus_tag="PLZ72_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNA34796.1"
                     /translation="PTNLDGDEQGDLRVHGGPHKAVLAYSAEHSEAWRAIEPELGEPG
                     AFGENLHVSGLTEADVCIGDRWAVGTALFEISQPRQPCWKVEHRWGRDGLVALMEETG
                     RTGWYLRVVTPGVVAADDTWTLEVRPNPRWSVAAANDVMHHRRDDLDAAASLASLDAL
                     APSWHRTLTKRAERLADGLADDVDLNARRVGPTD"
     gene            661..1320
                     /locus_tag="PLZ72_00465"
     CDS             661..1320
                     /locus_tag="PLZ72_00465"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HNA34797.1"
                     /translation="MATQGFEGRLERMVEGTFSRLFRSSVKPVEFGRKLQREMDARRS
                     VAVDGRTIVPNWYRFTVSETDHEQFAHIDGTLRRELADAAREHARDEGYSFVGPVEIE
                     VAGDPGMRTGLLEVDSRWVEGAGGLPPGSLVLPTGDRVPLGEYVVSVGRQADCTIVLA
                     DPNVSRVHAEVRPAGDGFVVADLGSTNGTKVNGVRVAEQELRDGDEVRFGNTAVRFEA
                     S"
     gene            1330..1821
                     /locus_tag="PLZ72_00470"
     CDS             1330..1821
                     /locus_tag="PLZ72_00470"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNA34798.1"
                     /translation="MPDQLLTVLKLCLLALLYLFFLRVLRAVWTEVNPPSAPSPAASG
                     AAGAATRSRRSSPRASTRSRRKQVVPTRLVVVEPAARAGTEYPLGPDMTVGRAQGCNL
                     VFDEQYVSQVHTRLLSREGSVFVEDLGSTNGTWVNGTRAVGQMPARLGDRIQIGNVVM
                     EVR"
     gene            1818..3344
                     /locus_tag="PLZ72_00475"
     CDS             1818..3344
                     /locus_tag="PLZ72_00475"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNA34799.1"
                     /translation="MTTLRSGAASLVGQVRSANEDSYLVTDDLVAVADGMGGHRAGEV
                     ASADTVDVLRDAAGSRSVQDLVAAVHRANRRIHERAADDDALRGMGTTVCVAGLVRYD
                     DREQVAVLNVGDSRAYLMADGELTRLTEDHSLVETLVREGRISAEEAEHHPQRNVITR
                     ALGVEPLVVVDAWLLEPVDGDRLLLCSDGLFGELGEDRIAELLAEDQDPEVVARRLAA
                     EADAAGGRDNITTVVVDVVDSGSAADEIEGRYRRISTPAVDLSDIDDERDRTETIMAV
                     VVPADPDGGSGVSDDLGDDPGDGSDPEHPLDDEADAGTLPEESGVDGVEGVAASEGDQ
                     VPAAGPPAVDDGDPVDDGDAVDAEGTVDAGRGSRWRTTLFIVTIVAVLAVMVGALVLW
                     SRIGWVVAAEDGIVVLQQGPRERILFIGPTDVERFEDIDVASLTDADRRQVDRGRTFG
                     SEREARAYIIGIQRHATTTTTSTTTTTTTTSTTTTVAPVPAAPDPFAPAPVPAPVPGP
                     "
     gene            3341..4693
                     /locus_tag="PLZ72_00480"
     CDS             3341..4693
                     /locus_tag="PLZ72_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNA34800.1"
                     /translation="MTAPAVTRRRPEPSPAVVALGTRRRTTELGLLVLAVLIILGAYA
                     LASLGRNASLPADIWPFLAVLLALLVGAHLANRRLARAADSLLLPIAALLNGIGYVFI
                     ARLDQDLAALQASWTAVGIVAYVVTLLAVRDVRLLQRYRYTAGLIGVLLLILPLLPGI
                     GRSINGARIWVSVGPVSFQPGEFAKIAFAVFFAGYLVERRELLSVATFRVGPFNTPDP
                     KHFAPILLAWGASLVVLIFQRDLGSALLFFLLFVVMLWVATGRVSYLLVGMGLFLVGA
                     FVSWLSFEHVQKRVSVWIDPWADPQGSGYQIIQAAYALAWGGTSGVGPGLGIAERIPY
                     DETDFIFAIIGEELGLLGTTAILCAFLLLAGTGLRVARESRDPFPSLLAVGLTVLIAF
                     QAFIIMGGVTRLLPLTGVTLPFVSYGGSSLVANYVLLALLVRISDQTARRAPERAVAM
                     "
     gene            4707..6212
                     /locus_tag="PLZ72_00485"
     CDS             4707..6212
                     /locus_tag="PLZ72_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNA34801.1"
                     /translation="MNRQIKALGVIVIICYVAVFAKLNQVQVLQAQAYSDRPDNTRQL
                     QRDFNQPRGDILTADGKTAATSEERRAALRYQRVYPDGELLSAVTGYYSFTLGSDGVE
                     RTYNEELAGRTPSLKLHRLSGFFSDTSSEGDVVLTVRKDLQETARDALGDRRGSVVAL
                     DPRTGAVLAMWSNPTFDPNLLSNNDAEAAAGAKALYEAAPTKPLLPKAYRERYFPGST
                     FKVVTATAGLTSGKVTVDSPTYPVVTSYTPPLTSKPISNFDGNACGGTLLPILARSCN
                     SSFAQMAAETTGPDPMIDAAEAAGFNQPVELDLPRPARSVYPTDFGAVVSRPEGSAPV
                     YEDTPKLAQTAIGQNDVRATPLQMAMVAAGAGNTGQIMAPHVMAEIRARNGDVVEKFA
                     PFPWKESMTPEVAATMREAMVGVVTDGTAAVMATPGWEVGAKTGTAQLGTKPPRSHGW
                     MIAFAGQPGQPAQVAVAVVVEDLPGVSAATGGSTAGPVAKAVLDKALGVSG"
     gene            6264..8213
                     /gene="pknB"
                     /locus_tag="PLZ72_00490"
     CDS             6264..8213
                     /gene="pknB"
                     /locus_tag="PLZ72_00490"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HNA34802.1"
                     /translation="MAEQEPRVLGDRYEIHQRLARGGMAQVYLARDRSLDRPVAVKEL
                     VPEFATDPSFVERFRREAQAAANLSHPNIVGVYDWGTQDGTYFIVMEYVDGPSLSQVI
                     RRDGPLHPRRAAELAGEVAAALGFAHSRGVVHRDVKPGNVLLTATGQSKVTDFGIARA
                     LSSPDDDLTQAGSVMGTATYFSPEQAQGLPVDPRSDLYSLGVVLYEMVTGRPPFSGDT
                     PLAIAYKHVQDQPAPPSTIVPEVPRGLEAIIMKLLQKRPDDRYPSAEALRADLNRYLD
                     GDLTAAERALTGTAAGVAAAGVAAAGVAAAATTVQPAVDPGTVQPIEDVDGGPDDAPK
                     SRTGLFVGVIAVLLAILAGLLFWFASSLRSDTVAVPALIGLPRAAAQKLLDDAGLEAK
                     FTEEPNATVAAGLVISQDPAAKTDVDKGSTVEVVVSTGVERVEVPDVVGKPRAEAEAA
                     LTAAGLKVTVNEVESEEAATGTVIAQNPAARTEVDRDSMVTLDVAKSPGEETVPDVAG
                     QNLQNAQAQLRAAGFKIAATKEAASATVASGLVISTDPAGGTTAKKGAAVTITVSTGV
                     EQVSVPNVVGQTETNATNTLQGRGFNVDTSTKTVPAGDSDIGRVISQDPTSGKQADKG
                     STVKIVVGVAATSTTSTPPTSSSTP"
     gene            8215..9156
                     /locus_tag="PLZ72_00495"
     CDS             8215..9156
                     /locus_tag="PLZ72_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNA34803.1"
                     /translation="MAGRRAGTDPIAKGAVALAVALTAAALLAGLLGFSSTLNPLDAL
                     LGRGAIVTVPDVSGRPQPGAEADLRRSGLVPKVRTSYSLTGTRGTVISQTPPAGSRVR
                     EGAEVEVVVSRGLNRVEMPAAVGQTLDAARAPLDEAGIDVTVTREPSETVPKGVVISQ
                     EPGPGVVVTAEDEVRLVVSDGAAPRAVPDVTGLALEGAAFRLGQAGFTVGAVTPVDDA
                     SAVLGSILRTDPAAGAMADRDTPIALAQAAGAPPVAVPDLNGRTADDASAALKALGLV
                     PNVIRGSGVDAVTAQDPAPTTMARPGTVVTVQIGTVG"
     gene            9158..10312
                     /locus_tag="PLZ72_00500"
     CDS             9158..10312
                     /locus_tag="PLZ72_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNA34804.1"
                     /translation="MGRARSDTAGVVDPDSEVELTAAESRSVRRRSDRDDRAVRVRGG
                     TPVTPIGGPEDVDPRRHPRATAIGLAVVGLLFVLTGFAIGTSMRDGERAVLAARVPVP
                     RLDGNTLEEATRTLEGLGLLVAVEYQPNEAVPTGTVFGQRPVAGSKLEVGTEVTLVVS
                     DGPAGVKVPDTTGFQGSEAVKLLEANGLAPVLQPTHDEKVRPGEVLRTDPAAGSRAAP
                     GATVAVLVSDGPAPRQVPAIAGTPIAEGFAALGRAGVGLGKVTATVVEGTPAGVVVST
                     DPPAGSAVPRDQPVDVVVTESAPSLKAPSVTGLLRSSAAEALADSPLVISYRNQTVAF
                     DDSRSGRVIAQSIPAGTPVSPGMQLQLTVAVAAAPPTTTTVPGATTTTVP"
     gene            complement(10330..10908)
                     /locus_tag="PLZ72_00505"
     CDS             complement(10330..10908)
                     /locus_tag="PLZ72_00505"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNA34805.1"
                     /translation="MAPRILVIDNYDSFVYNLVQYLGELGAEPEVHRDDALGLADVER
                     IDPDGILISPGPGTPADAGLSNEIIRTWGSRVPVFGVCLGLQCIGEVFGGEVVRAPEV
                     VHGKTSLIAHRGVGVFEGLPNPLEATRYHSLIVQRSTLPDVLEVTAETDDGLIMGLRH
                     RELDVEGVQFHPESVLTHAGHDLVGNFLRRCS"
     assembly_gap    11005..11104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11125..11460)
                     /locus_tag="PLZ72_00510"
     CDS             complement(11125..11460)
                     /locus_tag="PLZ72_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34806.1"
                     /translation="MASRRWVRLGLWLAVLAVTAVGVSLALSTPLAPTFGAVVLGALI
                     LRAGWFFLQQFATPPPDPPEAGTIRKVKLLYRCSICGTEVTMTAAPTEEPEPPRHCLE
                     DMELVAPIE"
     gene            complement(11465..12673)
                     /locus_tag="PLZ72_00515"
     CDS             complement(11465..12673)
                     /locus_tag="PLZ72_00515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNA34807.1"
                     /translation="MPDAVIIDIVRTPGGRRNGALSGWHPVDLAAETLKALAQRNDLD
                     PALVDDVIMGCVMQAGAQALNVGRNAVLAAGWPEEIPSTTVDRQCGSSQQAAHFAAQG
                     VMAGAYDVVIAAGVEVMSLVPMGASIGSGVGFPFGAAYDRYADVELIAGHKGLVGQGV
                     SAEIIANEWGLSREDLDAYGARSQALAEQATAEGRFENEIIPVVSKIRDKESGEIKVL
                     DTMVTADEGIRPGTTAEGLAKLKPSFLPEGKVTAGNSSQICDGASAALIMSADKAAAL
                     GLTPRARFHTFALAGVDPVRMLTGPIPATAKALEKSGLGIDDFDHIEVNEAFASVVLA
                     WEKEHHPDMAKVNANGGAIALGHPLGGSGTKLMATMLNELERIGGRYGLQTMCEGGGM
                     ANATVIERLG"
     gene            12828..14642
                     /locus_tag="PLZ72_00520"
     CDS             12828..14642
                     /locus_tag="PLZ72_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA34808.1"
                     /translation="MGSVTKADIDRLVDGTTVAREFVDTVARCGDSTALRWMQLDGTL
                     GSVSFAEFGDRAARAAAGLRSLGVQPGDRVVLMMRNIPEFHWLDMAVLLCGATPVSIY
                     NSSSADQVEYLVNHCGAVVAIVENQDFLAKFAEVRDRLTALRTIVTVVDPPELPDGVV
                     GHAVLTDADPVDLAEAASIGRPEDLATIIYTSGTTGSPKGVMISNYNVCWVLESGLMS
                     YGWTREEIKGKRVVSYLPMAHIAERIVSQYSLVLAGLEVTTCPDTSALLPHLIAVRPN
                     IAFGVPRVWEKLYAGVTAALAGDPEKAEKFNEAIAVGGPIREKMTWGEATDEEIATYE
                     FLDAVAFSTVRELVGMDQCEMAITGAAPIPADLLRWFRTIGIPLAEVYGMSENTGAMS
                     FERFKVKPGTVGLAMPGSELAVFPDGEVCCRGGHVFQGYLNDPDKTAEAIDDDGWLHS
                     GDIGELDDDGYLRIVDRKKELIITAGGKNISPANLEAELKSIPLVAQAAVIGDNRPFV
                     SALLVLDGEVAPGWAARHGIQYSSLEELAAHPDVQAAVADGVEQAMAGFNNAERVKKW
                     TLLSEEWLPDTDLLTPTFKLKRRGINAHFEKEIEALYS"
     gene            complement(14670..15806)
                     /locus_tag="PLZ72_00525"
     CDS             complement(14670..15806)
                     /locus_tag="PLZ72_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34809.1"
                     /translation="MGTRRALAAVTATTVVLALAASSCARAAETSEVSTDAPEKVSLQ
                     TSTTVARSASGTGSSATRSTKAGSGDLAQTAAQLFARLAADPSLLAQLTGMDISQIAA
                     LTGMDPAALASLNISPDAIRALAGALGGIDPTLVRRLGAGANLDPKLATTILMLAAQV
                     DPAAALAIKGVDPLAIASLISAATTVDPKVVASIGSVLKVADPNGLGRLADDKSSLAL
                     LAVMFGVAMRTDPSKFAQLGNAANLDPNVNFVLNSISGLAAGLTPQVVSSLNGLSKVL
                     GPDLLKALSAMMGILGRPDVAPVVQAAAKDPVVLVSTLGTFALLIPGLAEVLAPDVFA
                     NNPNARYGLLAGLIGVAIANLNGLDLNALAGTLGLPPLNPDFGR"
     gene            complement(15856..16329)
                     /locus_tag="PLZ72_00530"
     CDS             complement(15856..16329)
                     /locus_tag="PLZ72_00530"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNA34810.1"
                     /translation="MASPPERRVPRKEVATTVQLDVRPSLLLLRGFVHRCPACGGRHV
                     FRRWFQMEERCPTCTFLFERVEGHWIGSLGLNTTVVFGVMLVVLMAGTLAFYPDPPYG
                     ALLTAEVVIAVVGPLLFFPSSRMMWTAVDLLMRPLGPGEIDPRFVKVDPERDRMG"
     gene            complement(16363..16824)
                     /locus_tag="PLZ72_00535"
     CDS             complement(16363..16824)
                     /locus_tag="PLZ72_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34811.1"
                     /translation="MSRPDRIRLRGASVLALPLFVVAVIGLSLALASTLWAFGADQPV
                     GVAAVAAIAVPAAIGVAALVAVRGCFVDVDGSVVRDVVAWRTVRRVPTETIAAARVRR
                     GVWRLYVLELDGGTTVKLAGASPQQFPAWLLPDATQRDLEDLDVLLGAPSA"
     gene            16891..17595
                     /locus_tag="PLZ72_00540"
     CDS             16891..17595
                     /locus_tag="PLZ72_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA34812.1"
                     /translation="MAGAEPARILVVDDDRAIRDSLDRLLALEGHRVTTAADGASALD
                     RMRSGSVDLLVLDLMMPTVDGLTVCRVLRAEGDRVPILMLTARTETSDRVAGLDAGAD
                     DYLPKPFDPEELLARVRALLRRARPEPDGESDHAVLTVADLRLDPAARRAWRADRELE
                     LSRTEFDLLELLVFNAGIVLDRTRIYEEIWGYDFGPDSKNLAVYISYLRRKIDEGEDV
                     KLIHTVRGVGYTLRES"
     gene            17611..18993
                     /locus_tag="PLZ72_00545"
     CDS             17611..18993
                     /locus_tag="PLZ72_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA34813.1"
                     /translation="MSLRARFVLALVGLVAVATALIGAFSYRTTRAELLGQLDSSLQA
                     TTADLRRPGGVDRVLGPRSPASIRGDSDVIVQVVGADGTVYSMDGAAPLPVGASAEDL
                     LDEPEGTFRWQDDSVDGTAVRVLTTSLGGGRGVLQVGRSLSETRQVLAALRTRILVAS
                     LVVVAAAAAVGVVLARQLTRRLVRLTAAAEEVRRSGRLDVDVDTDGTDEAARLGVAFR
                     EMLGALARSREDQQRLVQDAGHELRTPLTSLRTNFYTLRRSLGPAGVGAGAAGGDTAR
                     ILDDLDQETEELSRLVDEVVEAATDRRGDEPEQPVDLGDLVERVAERARRRSGREVVV
                     HRLGPATVSGRPLALERAVTNLVENALKFDSGVQPIEVTCEGGRVEVADRGPGIAAAD
                     RPHVFDRFYRSTDARSRPGSGLGLSIVAEVVAGHGGRTFAAERAGGGAVVGFVLPVGP
                     EPAGRTGATA"
     gene            19005..19793
                     /locus_tag="PLZ72_00550"
     CDS             19005..19793
                     /locus_tag="PLZ72_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA34814.1"
                     /translation="MQDRDPRVRLELDGPVAVITNDNPDKHNAFDDAMDATLFDILAE
                     LSARPDIRAVVWRGEGKSWSSGRDVGSIGINNTDMTHHELMTRGIRGIQRLWDLEAPI
                     IVAIHGWALGGSFQRALLCDIRIASDDARFMLPEVGHGVIPDTGGVSVLYEMCGHGLV
                     SDMVLTGRRLSAQEALTHGIVSRVVTRDELDDTAMEMAQKIASSSAVAVKMARRVIGH
                     LARPAVRASMEDELIFQTFVNRSDDMAEMRAARAEGREPRYRGS"
     gene            19828..20709
                     /locus_tag="PLZ72_00555"
     CDS             19828..20709
                     /locus_tag="PLZ72_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA34815.1"
                     /translation="MTEPIGLPRPPAVGDTALRPGTFDGTAVFVTGAGTGLGKAIASE
                     FARLGADVVIASRKPEHLTAGAEAIEAIGARVLTVSCDIRDAESVAAAFDAAVDAFGL
                     PGVLVNNAAANFPVPAEDMSPNAWRTVVDITLNGTFLCCREFGRRHLAAGTPGSIVNI
                     GASYAWTGGPGFVHSAAAKAGVKNLTESLAVEWGPYGIQVNCLVPGLFPHEDMTDDIR
                     GNLDRTHDKDACQPALRVGERQELGWAATFLASPYGRFISGHTLVVDGANWQRRSMTN
                     PPVTTIRDQMGRPPFEP"
     gene            complement(20795..21850)
                     /locus_tag="PLZ72_00560"
     CDS             complement(20795..21850)
                     /locus_tag="PLZ72_00560"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNA34816.1"
                     /translation="MTDDHPDVTLTHEREVTEPVQLCLPGGTTLNPLARGWSRVPMHD
                     CNLAGTPGRKKRWDYWAVLGPTHTLSVTYADIDFLGITDVWWADLRSGRTGGRNRSVP
                     GAAGISLPDRPGTAPLRSAARHQVLDLTDEPGGTRLSAAWTEADGTPASMDVFVELPD
                     GHESLNVVIPWSDELFQYTSKHQARPAHGTLTIGDEVIAFGGDAGEAWGVLDVGRGRW
                     PHETRWNWGGGAGATTTGEVLGIQIGGKWTDGTGFTENGIIVDGRLHKLGGELRWDYD
                     WDRPLEPWRVVDPTGRMDITLTPVHDKHTKVDAKVLGTEVHQVFGTWTGTVVTDDGAE
                     LRVEDVLGFAEESRSRW"
     gene            21941..23248
                     /locus_tag="PLZ72_00565"
     CDS             21941..23248
                     /locus_tag="PLZ72_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34817.1"
                     /translation="MLRARSRSTGSARPSRSPGRRLRRFGAETFASLKVRNFRLFFAG
                     QGISQVGNWMTLIAQSLLVLSLTDSGIALGLLAVAQFGPILVLGPWAGLVADRSDKRR
                     LLLIVQSLAMVQSLLLAAVAFTDSPAWTVYAVALIGGFTVAFDNPARRAFVVEMVPEA
                     DVPNAVSLNSALMTSSRVIGPALAGLLITTAGYGWAFLLDGVSYVAVLVSLFLMRTEE
                     LRPSPPAERAKGQIREGFRYVRRTADLFVPLVMMGVVGTLAFNFGTVMPLFVTRDLDG
                     SDMVFTVLFSVVSVGSVLGALVAARRREVTVWNVGMSSLGFGATMAALAVVPSVATAM
                     PVALVLGVFSITFLTMSTAIVQMRAEPEMRGRVLALQAMLFLGSTPIGGPIVGWVAQV
                     FGARWSIALGAVACVAAGGWGMVTVGRGDHRVGSDPGREVLTS"
     gene            complement(23238..23870)
                     /locus_tag="PLZ72_00570"
     CDS             complement(23238..23870)
                     /locus_tag="PLZ72_00570"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNA34818.1"
                     /translation="METGLITGADGRRRCGWGALTEDYAAYHDLEWGRPVTDDVRLYE
                     KLCLEGFQSGLSWLTILRKRDNFRAAFAGFDPAAVAAYGPDDVERLMADAGIVRYRGK
                     IEAAVANASAVLDVQARHGSLAALIWSFEPARRGRPVPIELGEIATRTEQSTALAKEL
                     KRFGFRFIGPTTAYAAMQSVGVVNDHLRGCHVRSACDADRRATTVPTLTT"
     gene            complement(23890..24348)
                     /locus_tag="PLZ72_00575"
     CDS             complement(23890..24348)
                     /locus_tag="PLZ72_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNA34819.1"
                     /translation="MLITTGFDLPGYDITSVQGEIFGLTVRARNIGAGCIAGFRSIGG
                     GEIPEFTKLLAQSRSEAMARMVEEARKLGSTAIIGMRFDSGAIGQWSEICAYGTGVTV
                     NPLTDYARQQLEFVTATGAMPQQAGYSTKVSEWGPGPTVGTTPPAPGTPV"
     gene            complement(24404..25351)
                     /locus_tag="PLZ72_00580"
     CDS             complement(24404..25351)
                     /locus_tag="PLZ72_00580"
                     /inference="COORDINATES: protein motif:HMM:NF022794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HNA34820.1"
                     /translation="MRRRTRTVLAALATSTLLGLGLTACAPARTYTFNIATRGRITAD
                     VGEFARHVQATLDDRRGWSLGGSIRFVRTDGPSDFTVWLAAADTVPGFGRPCSSMWSC
                     RQGRNVIINQDRWLGASPSWPYGVETYRHYVVNHEVGHWMGFGHASCPAPWVRAPVMV
                     QQSKGGAAMGTCSFNVWPTPGELGGAGARHRVPVTPTGLLSPDGPFGNVDMAVVTRTT
                     EPDGSPGPPSAVELAGWTVDGDTVAPLTVVVLADGRPIASLPADRERLDVAAAVGYGP
                     SHGFHTTVPVGPDTVQVCVVAVGTGSGPALGQLGCQVVK"
     gene            complement(25454..25912)
                     /locus_tag="PLZ72_00585"
     CDS             complement(25454..25912)
                     /locus_tag="PLZ72_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA34821.1"
                     /translation="MSITADDRWAAAELLARYAELMDSGDFAAVGDLLAHASVATEDG
                     TVVATGADQIRSLYESTTRRHGDGTPLTAHVITNVIVDEVPGDPDEIAVRSRFTVLQG
                     TDRLGLQPVVVGRYDDRIRRIDGDWCFVHRTMIPERWGDVSEHLTFTPTT"
     gene            26173..26388
                     /locus_tag="PLZ72_00590"
     CDS             26173..26388
                     /locus_tag="PLZ72_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34822.1"
                     /translation="MGRPRVSDERRIATAVRLPESVHRRLQAAARDRDVSANLIVTRA
                     VEEYLDRLPSADAVLAPSRTGTPRTAS"
     gene            26385..27881
                     /locus_tag="PLZ72_00595"
     CDS             26385..27881
                     /locus_tag="PLZ72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNA34823.1"
                     /translation="MSPARSASPRNGRTRSATSTANFGSSRRESHDASAFYDRFVAPE
                     ISTDSTVNPPTELDVIYKTDARRMAEVPSDSVALVVTSPPYFAGKQYEEELGVGGVPA
                     DYFEYLELLHGVFAECKRVLEPGGRIAVNVANLGRRPYRSLSGDVTEILQDLGLLLRG
                     EVVWWKGRAAGGSCAWGTFQRPANPVLRDITERVVIASKGRFDRALTPAQRLERGLPS
                     TATISRDEFMEATTDLWEISPESATRVGHPAPFPVELPQRLVELYTYESDVVLDPFMG
                     SGSTAVAAVRTGRHFIGFDTDPEYVTIAESRVSGERERISTERPDPSGVGTVGSFRVE
                     LPAAPVEASSGDAPDPLAVAVRDGRQAREVAEILLGQCGFDNIRSEVKPRGLGIVLSF
                     VAADRTGADWAFDVSGTFTTNRSGLRRADTLWKSLGKAAVLHESRTSDDGVGMPLVLL
                     TTDAPAKGTSGHQALRVLRGQDRPVFDVVELLDPDGHARLRSYAENGL"
     gene            27954..29117
                     /locus_tag="PLZ72_00600"
     CDS             27954..29117
                     /locus_tag="PLZ72_00600"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA34824.1"
                     /translation="MGSDETPGADPIETTGAEHRTERWSAAGLWDPEAPDAQVRGALL
                     DYLESLGANVERMARAEAEGELVALGLDMLLEDGGLTVEQLATRMGVPVHEVVAAYRL
                     VGVEVNDPAAPMFEEAEIDFVALLTSTAGTFPDAATREILRALSTALSGLSAAAISAF
                     VGSVEDDLAGAGDPVAQARVTREAGLMGLDLAGGLRPLLRHHLRQGVRRQREGTHRSG
                     DRLTIVTAVGFVDLVGYTARTTDMDPEDLIGFIGSFRERTHEIVLAGGGQVVKHIGDE
                     IMFSAVDLAGVCRIALALLDGFDEIDTTPRGGVAAGRVVARHGDLYGPVVNLASRLAD
                     IAVPGEVLAPAERVTSAVEPDAGAEDLVFVPAGRRQLKGFDRPVAVVTVERPC"
     gene            complement(29291..29680)
                     /locus_tag="PLZ72_00605"
     CDS             complement(29291..29680)
                     /locus_tag="PLZ72_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA34825.1"
                     /translation="MLVVDASVLAPVVADVGSDGQRFRERLRGESVAAPDLLRVEVAS
                     VLRRHAAHGGLTPQQADQAMDDLLTFPLTVYPTSPLLPRVWELRPNVTAYDACYVALA
                     EAAGVPLVTADRRLAGAPGLRCAVDLV"
     gene            complement(29667..29897)
                     /locus_tag="PLZ72_00610"
     CDS             complement(29667..29897)
                     /locus_tag="PLZ72_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34826.1"
                     /translation="MPNVQVRDVPDAVHAALVRRAEEAGQSLQQFLTSQLAEIAATPT
                     VQDVLDRIQRRATGTLSARDAIEALDEERARR"
     gene            30099..30251
                     /locus_tag="PLZ72_00615"
     CDS             30099..30251
                     /locus_tag="PLZ72_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34827.1"
                     /translation="MLFDRALDALLAHHRRAEIDSAYAAYDEHPLDEADEWGDLASFR
                     SAAAAS"
     gene            30248..30574
                     /locus_tag="PLZ72_00620"
     CDS             30248..30574
                     /locus_tag="PLZ72_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNA34828.1"
                     /translation="MTDHPRRGELWWCELPEIGSRPVVVLSRDVAIPRLGRTLIAPCT
                     TTVRGLPSEVVLEPGVDPVPRLCAVNLDSVESVSVGVLVSRLGRLGDERMLAVCDALW
                     VAVDCH"
     gene            complement(30593..30982)
                     /locus_tag="PLZ72_00625"
     CDS             complement(30593..30982)
                     /locus_tag="PLZ72_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34829.1"
                     /translation="MVTRLALVAVLTVCACATGCAEDRTEAEVDWSLASPPVGREVDV
                     RAEFGGSSCSEFKDWDVAETSSSVTIRATVEYFGNECTADLVVEPMSIVLDEPPGDRR
                     LLGCRPESAVTAGSNRPTVDDCRETAS"
     gene            31132..31254
                     /locus_tag="PLZ72_00630"
     CDS             31132..31254
                     /locus_tag="PLZ72_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34830.1"
                     /translation="MPPLDRGLSREWPAPIAVRGHVRNKDDQDTNVIRFRRAAE"
     gene            complement(31388..32611)
                     /locus_tag="PLZ72_00635"
     CDS             complement(31388..32611)
                     /locus_tag="PLZ72_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNA34831.1"
                     /translation="MTSDAPTEAYVWIWLPDATEPVVCGRLDRRDARYVFSYGRSYLE
                     RPDPVPIYEPELPLDDFEHDGLDGPMPLSIDDAAPDSWGRSVINANLGAVGAELDPLV
                     YLLESGSDRVGAIDFQASPTEYIPRAPVHAPLEQLMTAADKIDEGETIDPDLAATLVN
                     GTPLGGARPKALIDDDGVKLLAKFSRRSDNFLWTQSEYIAMELARRAGLDVAPVRFVE
                     SMDRGVLLVERFDRSPAGARRRVVSAATILSMQTIVAAQHFSYVELADEVRANFIEPD
                     ATLRELFSRIMFNILVGNTDDHARNHAAFILADGLALTPAYDVSPQPRTGEVARHPRF
                     GDGDTGRESRIAALVEAAPIYHLTPEEGREIGDQQVATIRRDWDEVCDAAHLTTAQRK
                     GLLQGPILNPYAFYE"
     gene            complement(32601..32909)
                     /locus_tag="PLZ72_00640"
     CDS             complement(32601..32909)
                     /locus_tag="PLZ72_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA34832.1"
                     /translation="MATSRGTRAAVQILGDSVRAGRLRRGWSVGTLAERVGVSAPTIT
                     KIERGNPSVAIGTVFEAARLVGVELFGGNEDLAGQVVRNELALLPQRGRARRKARNDF
                     "
     gene            complement(32920..33345)
                     /locus_tag="PLZ72_00645"
     CDS             complement(32920..33345)
                     /locus_tag="PLZ72_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNA34833.1"
                     /translation="MSPKMAAHDAPKAGTKKQAANGKVFQVKVTLRDITPPIWRRVLV
                     DGSETLDEVHRVSQAAFGWWNSHLHEFRIGGRDYGVPDPDWDMSQVLDETRYRLDKLA
                     AEGDKFRYVYDFTEAQAGRSVGNRSRRVERIIGFRRSNS"
     gene            33335..33583
                     /locus_tag="PLZ72_00650"
     CDS             33335..33583
                     /locus_tag="PLZ72_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34834.1"
                     /translation="MGDIGQLTSGRSGSLVERADDDVGHPHRQGLEVEIAVRHEDAFS
                     PRGVRCRDRSDDCRIAHCVVDSGAADTRSDTFSIREVT"
     gene            complement(33873..34643)
                     /locus_tag="PLZ72_00655"
     CDS             complement(33873..34643)
                     /locus_tag="PLZ72_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34835.1"
                     /translation="MGAWGPGIFSDDLAADIRGDYRELLEDQVPDDEATGRILAAYRH
                     LDSDEVHVLWLALAAVQASLGRLDDEIKARALSVIDRGEGLEPWQEAGPQGLARREAA
                     LSKLRTQLTGPQPARRQVRRPWRHVTDLQPGDLLARVASNGDTCLLRVARIDDQRVGA
                     APVIELLDWKGQALPKDRQLRRLRPRYRDDGPHRPMTYRVARLRKKDPDWHDAGFERV
                     AQGLQQQGDDALPPWSYCGWSQLGDEVDRLVGPPKAAQ"
     gene            complement(34694..35239)
                     /locus_tag="PLZ72_00660"
     CDS             complement(34694..35239)
                     /locus_tag="PLZ72_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA34836.1"
                     /translation="MAAHVVVLDANVLYGIEVTDLVLTMATRHLYRPHWSPQILDEVT
                     RNLKLRADLDPAAIDRRIAQMNQALPSALDEPPASLIEEMPVNEKDRHVLALAVHVEA
                     PMIVTENLRDFPPELLADRGIEAIDVDTFVVGHVERDPIGVLGAIDAMAARRRRHPKT
                     SHDIIEVLAASLPHAMAALRE"
     gene            complement(35261..35695)
                     /locus_tag="PLZ72_00665"
     CDS             complement(35261..35695)
                     /locus_tag="PLZ72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HNA34837.1"
                     /translation="MSDVLEVRPDALGPEVLEAVVRALEGTGTSLPRLVGPDGDAIEL
                     PERIQSLLVSIVENLRAGNGVSVIPLHAELTTVQAAELLNVSRPYLIKQIEAGEVPHH
                     MVGTHRRLKLVDVLAYRDRLEARANEALDAMTADAEELGLYQ"
     gene            35782..35976
                     /locus_tag="PLZ72_00670"
     CDS             35782..35976
                     /locus_tag="PLZ72_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34838.1"
                     /translation="MITTDQEYDQSREKLASLVGSIASTEAGAAGDDEFRDLQLSGLR
                     SFADDLRTEIADYETQQKRR"
     gene            35973..36389
                     /locus_tag="PLZ72_00675"
     CDS             35973..36389
                     /locus_tag="PLZ72_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6228 family protein"
                     /protein_id="HNA34839.1"
                     /translation="MTLAEVRIAGEEGRALVLQHPQLDGDGGWRYTATLEFETGRASV
                     EVWDYGDPRLPEVFRQAADQWRGFQGAIEFASIEGQLEMALTHDGKGTISCVATVRQP
                     WPPTWRTTIELSFGAGAHAEQIARDVEAFFARAAPA"
     gene            complement(36404..36808)
                     /locus_tag="PLZ72_00680"
     CDS             complement(36404..36808)
                     /locus_tag="PLZ72_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34840.1"
                     /translation="MVDELDFAEHALAETLRLLALDPDGQVAALPDYVAVADELALTW
                     SDVQITLDRLVAGHRVEEAARCEVLDAISVFFEERSGEEHAAWWTTEAVEHDEGWETV
                     RLLARAALSRLDLEQRAPLLDWISYVPGSGPE"
     gene            36986..39682
                     /gene="mobF"
                     /locus_tag="PLZ72_00685"
     CDS             36986..39682
                     /gene="mobF"
                     /locus_tag="PLZ72_00685"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HNA34841.1"
                     /translation="MRVTTLKASADRLDGLSYYAGLAEDRGRPGLGRGPVDYYLDPDE
                     PPGRWWGSGRGALGLDGVVAGEDLRVVLEGRHPGSGAGLGRRFGDTSARGFDATFSAP
                     KSVSVLWALSPDSWVRAEVLAAHDAAVDAALGWFECHGAVTRRGTDGVFQVDTQGLTA
                     ALFRQHTSRTIDPQLHTHAVISAKVQDPTGRWLSLDARFLKYQQRTIGWIYDAALRAE
                     LTNRLGVTWAEPGDGARDLTCIPAEVNAEFSTRTTQVDAKLTELVRRWSYDHDGADPD
                     ARTIAGLQRAAVVASRPAKGHGFDPSVLRLGWEEHARAIGFDPDHLTADELTARQPEA
                     SRVPDEDLIDEALMRVSEESSTWLRADLARHLATLLDPRAGANGAALVAEVDRLAELA
                     ERLCAPLGPEPDGAPMLRADDRPVTEHVTDRRFSTHRILREEQDLQTWAETNTRHIPL
                     NGDARRDAAEAIAGHAPLVVLVGPAGTGKTRTTATAVAELQELGRPVIGLAPSGKAAD
                     VLCTEADCSTDTLAGFLTRHRHRRTKWPAGTTVILDEAGMTATADLAQLVHLVRVNRW
                     RLVAVGDPEQLPSVARGGVFAHWCNTVAHHTLDTPRRFSEPWEATASLGLRAGHPAAV
                     QLYTDHRRLHTAHPALVAGQVARAHQHHTASGRSVAITTNTAETATAINQEIQHLTRR
                     TGSPGVMLHDGTTARPGDRIATRRNDPRLRTDGGERVRNRHTWTVQAVHAGGAIVATD
                     DQRGTVTLPADYVNAHVELGWAVTGYGNQGDTVDIGIAVLEPTSSRNHAYVAMTRGRE
                     ANHALILDRTGTTDPAERLAEIITRPTRAHSALATQQRLHRDAGVEPPTLDDMAEPPK
                     TTVSGEPPTVAPELDEKVAAITRRLDALQRRAPSRDGQGLGL"
     gene            39716..40885
                     /locus_tag="PLZ72_00690"
     CDS             39716..40885
                     /locus_tag="PLZ72_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA34842.1"
                     /translation="MAASLVDGVMSASTFGRRASVSWKAPPMAIKKLPNGRWEASYRD
                     PAGRERVKHHRTRIDADRWLTTVKEQLHRGDYVDPRLGRRRFASFAEDWLAAAKAHVR
                     PKTWAHYESILRVHVLPTFADQPVGAISPADVRRYITDSVEAGSAPGTVRSARKVLRL
                     VLATAQADGAIRANPCDGSRVPASPKAEMVFLSVEQVERLATAIDERYATLVRLAAFT
                     GLRAGEICALRVGRVDLVSGRITVAESVTEVQGLGLHFSEPKTYERRSVTLPAFLADE
                     LAAHLEDRPGDPGTLVFTSPEGSTPNHKNFYRRHFKPAVSVADLPAETRFHDLRHTCA
                     ALCIALGAHPKAIQERLGHSSITVTLDRYGHLFPKLDETLTERLDDLHWAVVERR"
     gene            complement(40900..41778)
                     /locus_tag="PLZ72_00695"
     CDS             complement(40900..41778)
                     /locus_tag="PLZ72_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HNA34843.1"
                     /translation="MNPETGALRARLSHICREQLNPEAAAVVESLARPAVRMHHTDDP
                     TRSHLGGGALLVDSNQWPRWNDRPLSLVAVIDLAELSQFDTEPGLPSQGVLNFFYDDE
                     EQPWGFEPEDRGGWRVILVDPDDARAVGAPDGVETFVSIGLAPQQTLSIPGWEEPAVE
                     SVFPPHQDRTPAAEAARQRFFPMQDDWNEVVDVESSPNHQIGGWPRLQQGPIWRECDV
                     VSRGLPLGTSEQWRHAEPTFRVDREADWRLLLQLDTDDDAGWMWGDVGTLYFTFRRTL
                     PVAAACDEAWLVLQCG"
     gene            complement(41768..42190)
                     /locus_tag="PLZ72_00700"
     CDS             complement(41768..42190)
                     /locus_tag="PLZ72_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34844.1"
                     /translation="MKSLAWRHQFKSQPWSELRERYEDLASGEPAFGYMVEIVKSVEA
                     SLSRELLAAMTSMHDLLVTDRPIADPPLEVLRVSAPVGSLNPADNGDVVIEHLSHSGR
                     NDRVERSAAEAVPLFWRFVVEKFGIEGQQAENGSGDEP"
     gene            complement(42366..42439)
                     /locus_tag="PLZ72_00705"
     tRNA            complement(42366..42439)
                     /locus_tag="PLZ72_00705"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42403..42405),aa:Ala,seq:tgc)
     gene            complement(42540..43151)
                     /locus_tag="PLZ72_00710"
     CDS             complement(42540..43151)
                     /locus_tag="PLZ72_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34845.1"
                     /translation="MFVQVMHGKTSDPEALHRRLEVWEAELMPGAVGYLGSTGGCTSD
                     GDCILIARFEDRESARRNSDRPEQGAWWAETEKCFDGPVTFHDTEDVDLMTHGGVDSA
                     RFVQVMDGHVTDEARAKALERDSDDMLTQLRPDLLGALTAYYGDDEFTEVAYFTTEGA
                     ARDGEAAPMPEGATEMFTEWQSVMKVDRYLDINDPWLMTAPTR"
     gene            43311..43835
                     /locus_tag="PLZ72_00715"
     CDS             43311..43835
                     /locus_tag="PLZ72_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HNA34846.1"
                     /translation="MDATELLTDAIERIRHGVHEVVADLDPEQLSARVAPDANPIGWL
                     LWHLTRVQDAHVAEFLDAAQPYLDGGWAAELGLDPDPSDSGYGHSAAQVARVRIERPQ
                     RLIAYHDAVAARSLELLSGLSDHDLDRIVDESWDPPVTAGVRWVSIVEDSIAHLGQAS
                     YARGILEAQSAGAG"
     gene            43899..44522
                     /locus_tag="PLZ72_00720"
     CDS             43899..44522
                     /locus_tag="PLZ72_00720"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNA34847.1"
                     /translation="MSEQRRERRTERGLDRLVAFSDGVVAIAITLLVLPLLDVADDVS
                     SSDVWSDAWPRMQGFAITFAVVASFWVVHHRLFELIDDYNGRLVWANMLWLATIAFLP
                     FSSEVVGTGDLDDMTVRALYIGSLVACSVALAVIDVAILRSPDTWPDGERPDIDVTAV
                     VVRLGVMAAAFVIGTFVAPIGLWALLLLFLARPITGWIERGRSEPAT"
     gene            complement(44562..45752)
                     /locus_tag="PLZ72_00725"
     CDS             complement(44562..45752)
                     /locus_tag="PLZ72_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNA34848.1"
                     /translation="MNGTPAPAGPGAPPRLIPAAPEQSWLELFYDLVFVAAIVVMSTT
                     YSNYAGLDGVIWLALVFSMIWFTWLTTTLLLGTGLVTTTWTRTLLVTQMVLVLTVAIA
                     SDYSHEDNSRAVGPLFAAVLVTLALFFRAARRARPDLDVAYRGYGLRCLLSAAVFVAT
                     AAIGWPWWYPLLWLAGLLLFVAPTRSEQRRLPMDSHHTVHRFGEFTIIMLGEVFVKLG
                     ITATHEPLDQVDLFALPLATAVVFGIWWLYFTDVPASGLSERHGRRMAWVYLHFPMHL
                     SLVAAAVALAHTLVAEADETDTGQFEFGSIQYIVIPVAAILLAIGLIGLVGGTPRELA
                     RQRLRVFLWAAAALVIAEAVLLGVDSYDLETSTVIVAVVLALTARSIRRIGAAPEPVP
                     DAGT"
     gene            complement(45749..46282)
                     /locus_tag="PLZ72_00730"
     CDS             complement(45749..46282)
                     /locus_tag="PLZ72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA34849.1"
                     /translation="MTDPRLDEWELERLIARYADGVSRRDWTGLAELFEPDAALRLDL
                     IDRPARTLHGPDEILGFIAGAVERFDFFEFVALNVLGVLSTDPDGARRRGDGSVADGS
                     GARIRTHMCEVRRHGPDDPDAGCWSTAFGLYQDVVVRRGGKWRFAERSYRSLARTGTV
                     ATRDGLVFPPPRLRTEP"
     gene            complement(46279..47985)
                     /locus_tag="PLZ72_00735"
     CDS             complement(46279..47985)
                     /locus_tag="PLZ72_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNA34850.1"
                     /translation="MEPTDDPIPRIQRPRSADRYAPPSPQVARRRRLLVAGLALLVLL
                     GVAALVSRVVDGSDPGRTDAADSTGTGSGSGSGGGSGGKDDPNDKDTPKDLTQVPAEQ
                     TDQFVNPASSGRMWSAKVPGSLTFRGNPTRTYYGRGDVPSAPKVRWYYPKDSGMCSSS
                     PVGHTAKTWCGSGWTGQPAVFEREGRTWVVFGAYDKAVHFMDGETGEDILPPLPTGDI
                     IKGSVTVDPDGYPLVYSGSRDSYYHVIAIDRGPVAQELWKLSATAVSPTLWNNDWDGA
                     GIVADDFLFEGGENSQFHVVKLNRGYDAAGKVTVDPELVFNTPSWDQQLINDLAGNRA
                     KEFSIENSVSMSGNTAYWANSGGLVMGWDMGPLRTGGTPTQTLRFWAGDDTDASIVID
                     AEGFLYVAVEYERGNRRAAEVGQLMKLDPKNPTNPLVWKYDDRGANPAGFWATPALHK
                     GVVIAPANSGKVVGLDQQTGAELWSFKLAGPTWQSPVVVDDVLIQGDCNGVLHGYDVS
                     DPRTQPTEIWNVAIGGCIESTPTVWKGRIYFGTRAGRFFSLGDDGTLTSPTASAVGGG
                     GE"
     gene            complement(48044..49618)
                     /locus_tag="PLZ72_00740"
     CDS             complement(48044..49618)
                     /locus_tag="PLZ72_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNA34851.1"
                     /translation="MNEAAHRRTFAIISHPDAGKTTLTEKLLLYGGALGREAGAVKAR
                     GDRRSATSDWMELEQQRGISITSTVLQFPYRGQVVNLLDTPGHRDFSEDTYRVLTACD
                     AAVMVLDAAKGIEPQTLKLFEVCRDRGLPLITFVNKWDRPGLSPLELIDDIESHLSIM
                     CTPMTWPVGIAGDFRGVVDRSSGDFIRFTRTARGATEAPEELVDPDRAEAEEADAWLA
                     ARDELELLAGTEHDAELFLAGETTPMLFGSALTNFGVRLLLDTVIEQVPAPAARLDAA
                     GEPRPLGSPFSGFVFKVQANMDRAHRDRIAFLRVCSGRFERGMVVTHGRTGKPFATKY
                     AHSVFGQERETLEEAFPGDVVGLVNANDVRVGDTLFVQEPVTFPPIPSFAPEHFAVAR
                     TTDVGKSKQFRTGIQQLDEEGVVQVLRDPEFGDQAPILAAVGALQFEVATHRLINEFG
                     APVELTPTRFRVARRTDEASRAELRAMQGVDVLQRSDGELLAVFESVYWLERLESEHP
                     ELTLDKLVAEGELRQQ"
     gene            complement(49641..50837)
                     /locus_tag="PLZ72_00745"
     CDS             complement(49641..50837)
                     /locus_tag="PLZ72_00745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA34852.1"
                     /translation="MSEIRGSVEAGYEPVREAFERNFVENGDIGAGFCLYVDGAKVVD
                     LTGGVRDTDGTAYDDTTLQLVFSSTKGVTATCAHLLAQRGELDLDAPVADYWPEFSAA
                     GKADIPVRWLLSHQAGLVDVDRAMTVEEALDWTTICDALADSPPLWEPGTGYGYHAVT
                     YGWLVGEVVRRVAGVDFGEFVRREISEPLGLDLWVGLPDGEQHRVSPLINSGMPGLPA
                     AEPAGDADSQTDAPSLVGMLDMLLGEGNLLGRALTAPGGAFADDQVWNEARVRSAQIP
                     AANGVTNAASLARMYAALVSEVDGVRLLDADTLERAIAPQVSGPSTVPLLDIPFALGF
                     MTHSPLSPLLDGRSFGHYGAGGSLGFADPDRRVAGAYVMNAAQLAIAGDARTLGLLAA
                     VDQVVN"
     gene            complement(50911..51573)
                     /locus_tag="PLZ72_00750"
     CDS             complement(50911..51573)
                     /locus_tag="PLZ72_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34853.1"
                     /translation="MANGDGEVSGSRSSRPDPPTPRGRMLLALAASVLATASVSVPLA
                     YQAASARDGSSGTPATAPVTPSTVAPSSTAAPTTATPPASSVPGTVPTRVLPTQLERP
                     GDRIVWSRSASDPASHPLEGATLNGRVVVAFEPSVDSAAVVRVGFWVDHLDDVRPPEH
                     VDRQAPFTLTTSPSSDQGTAEGPVFDTTELKDGPHAVLVEAVGSDGRTVQRVSRFRVD
                     NG"
     gene            complement(51682..52335)
                     /locus_tag="PLZ72_00755"
     CDS             complement(51682..52335)
                     /locus_tag="PLZ72_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA34854.1"
                     /translation="MRVLLADDHQILRDGIRRGLESAGEDVVGEADNGEEAVALAIET
                     RPDIVLMDLSMPVLDGVGAARRIREEVPDSKVVVLTMHDDPAMTRAALQAGAAAYLTK
                     GTSFADVLDTLRRVQEGEETLSPRLAASMLEHLGPEPTRNDLLSDRQVEILQMIADGM
                     STKQAARALGITQKTVHNHLNATYRRLDTQSLTHAVLSAVRLGIIDLHSAPDDGEAA"
     gene            complement(52332..54218)
                     /locus_tag="PLZ72_00760"
     CDS             complement(52332..54218)
                     /locus_tag="PLZ72_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNA34855.1"
                     /translation="MSTADVSEAVAAMRALPHGGLDESTRLLGLPPTWRASVSPSIAV
                     LRWCALGYGLVFSAPAAFRGSYAAVVATAVCVFVTTWRTVMPVQLGSTHALRRIEPFI
                     DVVLFGVAMGVGREWASPFYFCLLIAVAVVALGWGFVTGGVALVVAVVSVAVSGAVGG
                     RSVPDMVTDQRDVAAVVTLVLAVVVAGYVRSRIKDAERRRIGLLGEVHHLSETNELLE
                     MLNVVARTLPDSLSLREALERIRQQLQDSFDARVICLMTFDDNSEEWVPKIADGCALR
                     PAYPRSDLPAPLGRALDHDGVIREGDLDPGSEALRPIVRTSRSGLYVRLTARGQVIGV
                     LGLEHPSQSRYDSHDAVLLSGLAEVLALTVDNARWFGRLRTLGAQEERIRIARDLHDR
                     LGQWLTYVSMELERIVAEDDPATADLMRLQGDVQSALDELRETLRQLRSGVTDAKPLS
                     VIALDVVNRFAERADVAATLTVVHPEDRAPVPVENELLRILQEALTNVDRHADAEHVD
                     VIWDVRGSEFELVVTDDGRGFESARGVRDSAYGLVGMRERADVVGARLTIDSTPGRGT
                     TVTVTAGPNPTPPRDRARTDTPVRRVNDSHTNDSHINDSHATDSRSTARSERSIDTNR
                     EVAS"
     gene            54418..55401
                     /locus_tag="PLZ72_00765"
     CDS             54418..55401
                     /locus_tag="PLZ72_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA34856.1"
                     /translation="MADPDETTSYPDRTVSPPEEAVTFPGARRPDRHRRVTASGIELH
                     VCEWGDASAPPLLLAHGGFDFTGTFDVFAPMLADAGWRVVSWDQRGHGDSDRAALYTW
                     DADVRDAAAVLDTLGSDPLPFIGHSKGGALMLHLANALPHRVSTMVNLDGLPSHQAMS
                     DVSDTERTRLLREQLTGWLDFRRSLVGRQRRPGTIDELAERRGRMNPRMTHDWLRYLV
                     TVGARRDDDGWRWKIDPTMRMGGFGPWRPQWSMLRLPGIGVPFLGVLGLELEVMGWGT
                     TPADVIPNLPPGGRLEALEGVGHFVHIEQPRLVADLVLEHLTAGAPAGGWS"
     gene            55398..56084
                     /locus_tag="PLZ72_00770"
     CDS             55398..56084
                     /locus_tag="PLZ72_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA34857.1"
                     /translation="MSGIRTLRHNRIDLALHELRGTDPAHPAARPLLLLHGLGERTPD
                     RVPTLVEWTGPVFGLDFTGHGCSTVPTGGGYSAEILVGDVDAALAELGPVTVLARGLG
                     SYIALLAAAARPELVRGAVLADGPGLVGGGVHPGSPTVPYVPEERVPAGEAPSSPDPF
                     ALAELARDPRPPDYVQTFVRFVLEGSDLDLPLWVAAVVRPEWLAAVADEPGVGQGTIG
                     DGLAHYASAV"
     gene            complement(56155..56373)
                     /gene="xseB"
                     /locus_tag="PLZ72_00775"
     CDS             complement(56155..56373)
                     /gene="xseB"
                     /locus_tag="PLZ72_00775"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNA34858.1"
                     /translation="MSEPTAPIDPEELGFAGAMAELDAIVAELESDALDVDVLADRVE
                     RAGALVAWCRDRIDGTRFRVEEILEQMD"
     gene            complement(56366..57688)
                     /gene="xseA"
                     /locus_tag="PLZ72_00780"
     CDS             complement(56366..57688)
                     /gene="xseA"
                     /locus_tag="PLZ72_00780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNA34859.1"
                     /translation="METATWSVGALHDAVNDVLAHVFGDVVWVEGEIVDLTRSAAGHV
                     YFRLVEPDDPDSGTPRSPAARPSLAVTLFDSQRQQVNRFLQAQGDPIRMTDGLRIRIG
                     GRLGTYAARSSLQLLMDRIDPAFTLGLLGADRARLIAALTREGLLRANAEVHLPSVAL
                     RVGLVTSVGSAAHADALHELESSGIGFHVQVFDARTQGADAPGSITRAIRAAVAHGVE
                     VVLLVRGGGATTDLIAFDDEAVARAIATSDVAVITGIGHETDRSVADEVAHTAQKTPT
                     AAAQLLVRTVRDAERRILDRWAAARAGTHGGLDRAEVRLDRIRERTGARAVARLDRSA
                     LELDHRVRRIHAGARRRIERADARTELIAVSTAALDPALAMRRGWSITRGADGSLVRS
                     VDALATNQELITTLADGTVTSSVLGITPAARDPTVRAEGADPGGEPHV"
     gene            complement(57695..58159)
                     /locus_tag="PLZ72_00785"
     CDS             complement(57695..58159)
                     /locus_tag="PLZ72_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA34860.1"
                     /translation="MHALIATDGSDVSIEAARRAVALLNPSKVTLLTVADTSVAEDSG
                     AGGFEGNLLSPSEAEQARAAILDEGDDELADTIEAMGVDPAIVERKLVEGASGQMIVH
                     VAEESGVDVIVVGSHGKGWFKRVVIGSISEYVVHHSHIPVLVIRNEPADGKD"
     gene            complement(58212..60173)
                     /locus_tag="PLZ72_00790"
     CDS             complement(58212..60173)
                     /locus_tag="PLZ72_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA34861.1"
                     /translation="MRRCRFLRVSAAMFRMMHQDPTQVAGRTLDRALVRRVGRFARPY
                     RSQLVGFVVMIAAGAGLALVPPLLFRTIIDTAIPDGNRAQLVGLGAVVVVAAALTAAA
                     SLLERFWSARIGEGLIYDLRRALFDHVQRMPLQFFTRSQTGAIVSRLNNDVIGAQRAL
                     TGTLGTVVSNVITLGFTLVAMIALDWRITLVAVVLLPLFLVPSKRVGRRLQSMTRDSM
                     QLNAGMNAQMTERFGVSGAQLVKLFGDHDRETDEFATRAVAVRDIGIRSAMYSRWFMV
                     MMGLVGAIGTAAVYLLGGLQVIDGNITIGTLVALTALVTRIYDPLTSLTNARVDVMSA
                     FVSFERVFEVLDSPNPLADRPGAAELTDPTGSIRFEHVWFRYPGADEGSIRSLETGAV
                     ETEHPDGPPLVLHDIDLDIAAGTTVALVGPSGAGKSTIASLIPRLYDATEGSVLLDGH
                     DVRDLTQASVRRAIGVVSQDPHLFHATVGENLRYARPDATDEQLREACRAARVLDVVD
                     AMPDGFDTMVGERGYRLSGGEQQRLAIARLILKDPAIVVLDEATSALDTENEAAIQAA
                     LADALEGRTAVVIAHRLSTVVGADLIVVVDGGRIVQRGTHRELLAQGGLYADLYRVLV
                     SGSDPDTPDTAAATDVPAGSGPVRSGTGG"
BASE COUNT         8755 a        21022 c        21386 g         8943 t
ORIGIN      
        1 accgaccaac ctcgacggcg acgaacaggg tgacctgcgg gtccatggcg ggccccacaa
       61 ggcggtgctc gcctactccg ccgagcactc cgaggcctgg cgggccatcg agccggagtt
      121 gggcgagccc ggggccttcg gtgagaacct ccacgtgtcg ggcctcaccg aggccgacgt
      181 gtgcatcggc gaccgctggg cggtcgggac cgcgctgttc gagatctccc agccccgcca
      241 gccgtgctgg aaggtggagc atcgctgggg gcgcgacgga ctggtggccc tcatggagga
      301 gacgggacgc accgggtggt acctgcgggt ggtgaccccg ggcgtcgtcg ccgccgacga
      361 cacctggacc ctcgaggtca gaccgaaccc ccgctggagc gtcgccgcgg cgaacgacgt
      421 catgcaccac cggcgcgacg atctcgacgc cgccgcatcc ctggcgtcgc tcgacgctct
      481 cgccccgtcg tggcaccgga cgctcacgaa gcgggccgag cggctcgcgg acggcctcgc
      541 cgacgacgtg gacctgaacg cccgacgcgt cgggcccacc gactgatccg gccggccacc
      601 cagggtcgcc ccgacccggt gccccgggtg gtgaccgctg acgtcgctat cgtggggccg
      661 atggctaccc agggcttcga gggccgcctc gaacgcatgg tggaagggac cttcagccgt
      721 ctcttccgct cctcggtcaa gccggtcgag ttcggtcgca agctccaacg cgagatggac
      781 gcccgtcgtt ccgtcgcggt cgacgggcgc acgatcgtcc cgaactggta ccgcttcacc
      841 gtctcggaga ccgaccacga gcagttcgcc cacatcgacg gcaccctgcg tcgcgagctg
      901 gccgacgcgg cccgcgagca cgcccgcgac gagggctact ccttcgtcgg ccccgtcgag
      961 atcgaggtcg ccggagaccc ggggatgcgg accgggctgc tcgaggtcga ctcccgatgg
     1021 gtcgaagggg ccggcggtct gcccccgggg tcgctggtgc tgccgaccgg tgaccgggtt
     1081 cctctcggcg agtacgtcgt gtcggtcggt cgtcaggccg actgcacgat cgtgctggcc
     1141 gacccgaacg tcagccgggt ccacgccgag gtccgtcccg ccggtgacgg gttcgtcgtg
     1201 gcggacctgg gctcgaccaa cggcaccaag gtcaacgggg tgcgggtggc cgaacaggag
     1261 ctccgcgacg gcgacgaggt ccgcttcggc aacaccgccg tccgcttcga ggcctcgtag
     1321 gacggacccg tgcccgacca gctgctcacc gtcctgaagc tgtgcctgct ggcgctgctg
     1381 tatctcttct tcctgcgcgt gctccgggcg gtgtggaccg aggtgaaccc tccctcggcg
     1441 ccctccccgg cggcgtcggg tgcggccggc gccgcgaccc gctcccgacg gtcctccccc
     1501 cgggcctcga cccgcagccg gcgcaagcag gtggtgccga cccgcctggt cgtggtcgaa
     1561 cctgcggcgc gggccggaac cgagtacccg ctcggcccgg acatgaccgt cggccgcgcc
     1621 cagggctgca acctggtctt cgacgagcag tacgtctcgc aggtgcacac ccgcctgctg
     1681 agccgcgagg gctcggtgtt cgtcgaggac ctcggatcga ccaacgggac gtgggtcaac
     1741 ggcacccgcg ccgtgggcca gatgccggcc cgactcggtg accgcatcca gatcggcaac
     1801 gtggtcatgg aggtccgatg accacgctgc gttcgggcgc ggcgtcgctc gtgggacagg
     1861 tccggagcgc caacgaggac tcgtacctgg tgaccgacga cctggtggcg gtcgccgacg
     1921 gcatgggagg acaccgcgcc ggcgaggtgg cgtccgccga caccgtcgac gtgctgcgcg
     1981 acgcggccgg gtcgcggtcg gtgcaggacc tggtcgcggc ggtgcaccgg gccaaccgtc
     2041 ggatccacga gcgcgccgca gacgacgacg ccctgcgggg gatgggcacc accgtgtgcg
     2101 tcgccgggct cgtccgctac gacgaccgcg agcaggtcgc ggtgctcaac gtcggcgatt
     2161 cgcgggccta cctgatggcc gacggcgagc tgacgcgcct gaccgaggac cactccctgg
     2221 tcgagacgct ggtgagggag ggccgcatct ccgccgagga ggccgagcac cacccccagc
     2281 gcaacgtgat cacccgggcc ctgggcgtcg agcccctcgt cgtcgtcgac gcctggctgc
     2341 tcgaaccggt cgacggcgac cgactgctgc tgtgcagcga cggtctgttc ggcgagctgg
     2401 gcgaggaccg catcgccgaa ctgctggccg aggaccagga ccccgaggtc gtcgcccgcc
     2461 ggctcgcggc cgaggccgac gcggccggtg ggcgcgacaa catcaccacc gtcgtggtcg
     2521 acgtggtcga ctccggcagc gccgccgacg agatcgaagg gcgctaccgg cgcatcagca
     2581 cgccggcggt cgacctgtcc gacatcgacg acgaacggga ccggaccgag acgatcatgg
     2641 cggtcgtggt cccggccgac cccgatggtg gctccggggt ctcggacgat ctcggagacg
     2701 atcctgggga cgggtccgac ccggagcacc cgctcgatga cgaagccgac gcgggcacgc
     2761 tcccagagga gtccggcgtc gacggggtcg agggagtcgc ggcatcggaa ggtgaccagg
     2821 tcccggcggc aggaccgccg gcggtcgacg acggggaccc ggtcgacgat ggggacgcgg
     2881 tcgacgccga ggggaccgtc gatgccggtc ggggcagccg gtggcggacc accctcttca
     2941 tcgtgaccat cgtggcggtg ctcgcggtca tggtcggagc gttggtgctg tggtcgagga
     3001 tcgggtgggt cgtcgcggcc gaggacggga tcgtggtgct ccagcagggg ccgagggagc
     3061 ggatcctgtt catcggcccg accgacgtgg agcgcttcga ggacatcgac gtcgcctcgc
     3121 tcaccgacgc ggaccgccgt caggtcgacc ggggtcgaac gttcggatcc gaacgcgagg
     3181 cccgggccta catcatcggc atccagcgcc acgccacgac caccacgacg tcgaccacca
     3241 cgaccaccac cacgacgtcg accaccacca cggtggcgcc ggtgccggcg gcaccggatc
     3301 cgttcgcgcc cgctcccgtg cccgcacccg tcccgggtcc gtgaccgcac cggccgtcac
     3361 ccggcgccgc cccgagccct cgccggcggt cgtggccctc ggcacccggc gtcgcacgac
     3421 cgagctcggc ctgctcgtgc tggccgtgct gatcatcctc ggcgcgtacg cgctggccag
     3481 cctgggtcgc aacgcctcgc tccccgcgga catctggccg ttcctggcgg tcctcctggc
     3541 cctgctggtc ggggcgcacc tggcgaaccg acgtctggcc cgggctgcgg actcgctgct
     3601 gctgcccatc gccgcgctgc tcaacggcat cggctacgtg ttcatcgccc ggctcgacca
     3661 ggacctggcc gcgctccagg cgtcgtggac cgcggtcggc atcgtcgcct acgtcgtgac
     3721 cctgctcgcg gtgcgcgacg tccggctgct gcagcgatac cgctacaccg ccgggctgat
     3781 cggcgtgctg ctgctgatcc tcccgctgct gccgggcatc gggcgcagca tcaacggggc
     3841 ccgcatctgg gtgtcggtcg ggccggtcag cttccagccc ggggagttcg ccaagatcgc
     3901 cttcgccgtc ttcttcgcgg gctacctggt cgagcgtcgg gagctgctga gcgtcgccac
     3961 gttccgggtc ggtccgttca acaccccgga ccccaagcac ttcgccccga tcctgttggc
     4021 gtggggggcg tcgctggtcg tgttgatctt ccagcgcgac ctcggctcgg cgctgctgtt
     4081 cttcctcctg ttcgtcgtca tgttgtgggt ggcgacaggc cgcgtgagct acctgctcgt
     4141 cgggatgggg ctcttcctgg tcggggcgtt cgtcagctgg ctcagcttcg agcacgtcca
     4201 gaagcgcgtc tcggtgtgga tcgacccgtg ggccgatccg cagggatccg gataccagat
     4261 catccaggcc gcctacgcgc tcgcgtgggg aggaacctcc ggggtcgggc ccgggttggg
     4321 catcgccgag cggatcccct acgacgagac cgacttcatc ttcgcgatca tcggcgagga
     4381 gctcggcctg ctcggcacga ccgccatcct gtgcgccttc ctgctgctgg ccggcaccgg
     4441 actccgggtg gcccgcgagt cgcgggaccc gttcccgtcg ctgttggcag tggggctcac
     4501 cgtgctgatc gcgttccagg ccttcatcat catgggtggc gtcacccgac tgctgcccct
     4561 caccggggtg accctgccgt tcgtctccta cggcggcagc tccctggtgg ccaactacgt
     4621 gctgctggcg ctcctggttc ggatctcgga ccagacggcg cgtcgcgccc cggagcgggc
     4681 ggtggcgatg tgagcggggt ccgtcgatga accgtcagat caaggcgctc ggcgtgatcg
     4741 tgatcatctg ctacgtcgcc gtgttcgcca agctcaacca ggtccaggtc ctgcaggcgc
     4801 aggcctacag cgaccgcccc gacaacaccc gccagctcca gcgcgacttc aaccagcccc
     4861 gcggcgacat cctcaccgcg gacggcaaga ccgccgccac ctccgaggag cgacgggccg
     4921 cgttgcgcta ccagcgggtg taccccgacg gcgagctgct ctcggccgtg accggctact
     4981 actcgttcac gttgggctcc gacggtgtgg agcgcaccta caacgaggag ctggcggggc
     5041 ggacaccgtc gctcaagctg caccggctgt cgggcttctt ctccgacacc tcgagcgagg
     5101 gtgacgtggt gctgacggtg cgcaaggacc tccaggagac cgcccgcgac gccctcggtg
     5161 accgtcgggg gtcggtggtg gcgctcgacc cgcgcaccgg ggcggtgctc gccatgtggt
     5221 cgaatccgac cttcgacccg aacctgctgt ccaacaacga cgccgaggct gcggccgggg
     5281 ccaaggccct gtacgaggcg gcgccgacca agccgctgct gcccaaggcg taccgcgagc
     5341 gctacttccc cggctcgacg ttcaaggtcg tgaccgccac cgctggtctg acctcgggca
     5401 aggtcaccgt cgactcgccg acctatccgg tggtgacgtc gtacaccccg ccgctgacca
     5461 gcaagccgat ctccaacttc gacggcaacg cctgcggtgg gacgctcctc cccatcctgg
     5521 cccgctcctg caacagctcc ttcgcccaga tggccgccga gaccaccggt ccggatccga
     5581 tgatcgacgc ggccgaagcg gccgggttca accagccggt cgagctcgac ctgccccgcc
     5641 cggcgcggtc ggtgtacccg accgatttcg gagcggtcgt cagtcggccc gagggttcgg
     5701 ctccggtcta cgaggacacg cccaagctgg cccagaccgc catcggccag aacgacgtgc
     5761 gcgccacgcc gctgcagatg gccatggtcg ccgccggtgc cggcaacacc ggccagatca
     5821 tggctccgca cgtcatggcc gagatccggg cccgcaacgg agacgtcgtc gagaagttcg
     5881 ctcccttccc gtggaaggag tcgatgacac ccgaggtcgc ggcgaccatg cgcgaggcga
     5941 tggtcggcgt cgtcaccgat ggcacggcgg cggtcatggc cacgccgggc tgggaggtcg
     6001 gggccaagac gggaaccgcg cagctcggaa ccaagccgcc ccgctcgcac ggctggatga
     6061 tcgccttcgc cggtcaaccc ggtcagcccg cacaggtcgc ggtcgcggtg gtcgtcgagg
     6121 accttcccgg cgtcagcgcc gccaccggcg gatcgaccgc cggtcccgtc gccaaggccg
     6181 tcctggacaa ggcgctcggg gtgtcgggct gagcgtgccc atgacacgat ccgacgggtc
     6241 ggggactcta cgctggcggc gccatggctg agcaggaacc acgggtcctc ggggaccgct
     6301 acgagatcca tcaacggctc gcacgcggcg ggatggccca ggtgtacctg gcccgtgacc
     6361 gcagcctcga ccgcccggtc gcggtcaagg agctggtgcc cgagttcgcc accgacccga
     6421 gcttcgtcga acgcttccgc cgcgaggccc aggccgccgc caacctgtcg cacccgaaca
     6481 tcgtcggcgt ctacgactgg ggaacccagg acggcaccta cttcatcgtc atggagtacg
     6541 tcgacggccc gtcgctgtca caggtcatcc gccgcgacgg cccgttgcac ccccggcggg
     6601 ccgccgagct cgccggcgag gtcgccgccg ctctgggctt cgcccactcc cgcggcgtcg
     6661 tgcaccgcga cgtgaagccg ggcaacgtcc tgctcaccgc caccggccag tccaaggtca
     6721 ccgacttcgg catcgcccgg gcgctctcgt ctcctgacga cgacctgacc caggccggct
     6781 cggtcatggg gaccgccacc tacttctcgc ccgagcaggc ccagggactg ccggtcgatc
     6841 cgcggtcgga cctgtactcg ctcggcgtcg tcctctacga gatggtgacc ggccggcccc
     6901 cgttcagcgg cgacaccccg ctcgccatcg cctacaagca cgtccaggac cagccggccc
     6961 cgccgtccac gatcgtcccc gaggtccccc gcggcctcga ggcgatcatc atgaagctcc
     7021 tccagaagcg tcccgacgac cgctacccgt cggccgaggc gctgcgggcg gatctcaacc
     7081 gctacctcga cggcgatctc accgctgccg agcgggccct caccggcacc gcggccggcg
     7141 tcgccgcagc cggtgtggcc gcggcaggcg tcgccgccgc ggccaccacc gtgcaaccgg
     7201 cggtcgatcc cggcacggtg cagccgatcg aggacgtcga cggtgggccc gacgacgccc
     7261 cgaagtcgcg gaccgggctc ttcgtggggg tcatcgccgt gctgctcgcc atcctggccg
     7321 gactgctgtt ctggttcgcc agcagcctgc ggtccgacac cgtcgccgtc cccgccctga
     7381 tcggcctgcc gcgggccgcc gcacagaagc tgctcgacga cgccggcctc gaggccaagt
     7441 tcaccgagga gccgaacgcg acggtcgccg ccggtctggt catctcccag gacccggccg
     7501 ccaagaccga tgtggacaag gggtcgacgg tcgaggtggt cgtgtcgacc ggcgtcgagc
     7561 gcgtcgaggt ccccgacgtc gtcggcaagc cccgcgccga ggcagaggca gcgctcaccg
     7621 cggcgggcct caaggtcacg gtcaacgagg tcgagagcga agaggcggcg accggaacgg
     7681 tcatcgccca gaaccccgcg gcgcggaccg aggtcgaccg ggactcgatg gtgaccctcg
     7741 acgtggccaa gtcgcccggc gaggagaccg tccccgacgt cgccggccag aacctccaga
     7801 acgcccaggc ccaactccgc gccgccggct tcaagatcgc cgccaccaaa gaggcggcga
     7861 gcgcgacggt cgcgtctggt ctggtcatct cgaccgaccc ggccgggggg acgaccgcca
     7921 agaagggcgc ggcggtgacg atcacggtgt cgaccggtgt cgagcaggtc agcgtgccga
     7981 acgtcgtcgg acagaccgag accaacgcga ccaacaccct ccagggccgc gggttcaacg
     8041 tcgacacctc caccaagacc gttcccgcgg gcgactccga catcggacgg gtcatcagcc
     8101 aggacccgac ctcgggcaag caggccgaca agggctcgac ggtcaagatc gtggtcggcg
     8161 tcgcggccac gagcaccacc tcgaccccac cgacgtcgtc gtccaccccc tgacatggcc
     8221 gggcggcggg ccggcaccga ccccatcgcc aagggagcgg tggccctggc ggtggctctg
     8281 accgcggcgg ccctgctcgc cgggctgctc gggttctcct cgacgctcaa cccgctcgac
     8341 gcgctcctcg gacggggagc gatcgtgacc gttcccgacg tgtccggccg tccgcagccc
     8401 ggagcggagg ccgacctgcg ccggtccggg ctcgtgccga aggtccgcac cagctacagc
     8461 ctgaccggca cccggggcac cgtgatctcc cagacgcccc cggcgggctc gcgggttcgg
     8521 gagggtgccg aggtcgaggt cgtggtcagt cgcggcctca accgggtcga gatgcccgcc
     8581 gcggtcggac agaccctgga tgcggcccgg gcgccgctcg acgaggccgg aatcgacgtg
     8641 acggtcaccc gggagccgag cgagaccgtg cccaagggcg tcgtgatcag ccaggagcca
     8701 ggcccggggg tcgtggtcac cgccgaggac gaggtccgtc tggtcgtctc cgacggggcc
     8761 gccccccgtg cggtgcccga cgtgaccggc ctcgccctcg agggagcggc gttccggttg
     8821 ggtcaggccg gcttcaccgt cggagcggtg accccggtcg acgacgcctc ggcggtgctc
     8881 ggatcgatcc tgcgcaccga ccccgccgcg ggggcgatgg cggaccgtga cactccgatc
     8941 gcgctcgccc aggccgccgg agctcctccc gtcgcggtgc ccgacctgaa cggccggacc
     9001 gcggacgacg cctccgcggc gctgaaggct ctcgggctgg ttcccaacgt gatccggggc
     9061 tccggcgtcg acgcggtcac ggcccaggac ccggccccga ccacgatggc ccgtcccgga
     9121 accgtggtca cggtacagat cgggacggtg ggctgagatg ggacgagcac gctccgacac
     9181 cgccggcgtc gtcgacccgg actcagaggt cgagctgacc gcggcggagt cgcgctcggt
     9241 acgtcggcgc tccgaccgtg acgaccgggc ggtccgggtc cggggcggaa cgcccgtcac
     9301 gccgatcgga ggtccggagg acgtcgaccc ccgtcgccac ccccgcgcca ccgcgatcgg
     9361 tctggccgtc gtcggcctgc tgttcgtgct caccggcttc gcgatcggca cctcgatgcg
     9421 agacggcgaa cgggccgtgc tcgccgcccg cgtcccggtt ccccgattgg acggcaacac
     9481 cctcgaggaa gccacccgga ccctcgaggg cctggggctg ctggtcgccg tggagtacca
     9541 gcccaacgag gccgtgccga cggggacggt gttcggccaa cgcccggtcg cgggatccaa
     9601 gctcgaggtc gggaccgagg tgaccctggt cgtcagcgac ggtcccgccg gtgtcaaggt
     9661 ccccgacacg accggcttcc agggatccga ggcggtgaag ctcctcgagg ccaacggact
     9721 ggccccggtc ctgcagccga cccacgacga gaaggtccgg cccggtgagg tgctgcggac
     9781 cgatcccgcc gccggcagcc gagcggctcc gggagcgacc gtggccgtgc tcgtctccga
     9841 cggccccgcg ccgcgacagg tgcccgccat cgcaggaacg cccatcgccg agggcttcgc
     9901 cgccctcggg cgtgcgggag tcggcctggg caaggtcacc gccaccgtgg tcgagggaac
     9961 ccccgccggt gtggtcgtct ccaccgaccc gccggccggc tcggcggttc cccgggacca
    10021 gccggtggac gtcgtcgtca ccgagtcggc tcccagcctg aaggcccctt cggtgaccgg
    10081 actgctgagg tcgtcggccg ccgaggcgct tgccgacagc ccgctggtga tcagctaccg
    10141 gaaccagacc gtcgccttcg acgacagccg gtccgggcgg gtcatcgccc agagcatccc
    10201 ggcgggaacc ccggtgtcgc cgggcatgca gctgcaactg acggtggcgg tcgcggccgc
    10261 cccgccgacc accaccacgg ttccgggggc cacgaccacg accgtcccct gagcctcgac
    10321 ctggccgcgt cagctgcagc ggcgcaggaa gttgccgacc aggtcgtgac cggcgtgggt
    10381 cagcaccgac tccgggtgga actggacgcc ttcgacgtcg agctcgcggt gacgcaaccc
    10441 catgatcaac ccgtcgtcgg tctccgcggt gacctccaac acgtcgggca acgtcgaacg
    10501 ctggacgatc agcgagtggt agcgggtcgc ctcgagcggg ttgggcaacc cctcgaagac
    10561 ccccactccc cggtgtgcga tgagggaggt cttgccgtgc accacctctg gcgcacgcac
    10621 cacctcgcca ccgaacacct caccgatgca ctggaggccc aggcacacgc cgaacacggg
    10681 gacccgcgaa ccccaggttc ggatgatctc gttggacaga cccgcgtcgg ccggggttcc
    10741 cggtccgggg ctgatgagga tgccgtcggg gtcgatccgc tcgacgtcgg ccagccccag
    10801 ggcgtcgtct cggtgcacct cgggttcggc tcccagctcc cccaggtact gcaccaggtt
    10861 gtagacgaac gagtcgtagt tgtcgatcac caggatccgg ggcgccacga ccgagagcgt
    10921 acgggttgcc cgcggcgacg gaacaaccgt taccctcacc gccatggcga agccggccaa
    10981 gcgcagggtg gaggggaccg gtcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnntcgatg ggagccacca gctctcactc gatgggagcc accagctcca tgtcctccag
    11161 gcagtgtcgg ggtggctcgg gctcctcggt cggcgcggcg gtcatggtga cctcggtgcc
    11221 gcagatcgag caccggtaga gcagcttgac cttgcggatc gtgcccgcct cgggcggatc
    11281 cggaggaggg gtcgcgaact gctggaggaa gaaccagccg gcccgcagga tcagcgcacc
    11341 cagcaccacc gcgccgaagg tcggcgcgag cggggtgctg agagccaggc tcaccccgac
    11401 ggcggtcacg gccaggaccg cgagccacag gcccagccgg acccaccgtc gggatgccaa
    11461 cctgtcagcc caggcgctcg atgacggtgg cgttggccat gccgccaccc tcgcacatcg
    11521 tctggaggcc gtagcggccc ccgatgcgct cgagctcgtt gagcatggtg gccatcagct
    11581 tggtgccgga tccgccgagc gggtgaccga gcgcgatggc gccgccgttg gcgttgacct
    11641 tcgccatgtc gggatggtgc tccttctccc acgccaggac gaccgaggcg aacgcctcgt
    11701 tcacctcgat gtggtcgaag tcgtcgatgc ccaggccgct cttctcgagc gccttcgcgg
    11761 tggcggggat ggggccggtg agcatgcgga cggggtcgac gccggccagg gcgaaggtgt
    11821 ggaaccgggc ccggggcgtc aggcccagcg cggcggcctt gtcagcgctc atgatcagag
    11881 cggccgaggc gccgtcgcag atctgggagc tgttgccggc ggtcaccttg ccctcgggca
    11941 ggaacgacgg cttgagcttg gccagtccct cggcggtggt gccggggcgg atgccctcgt
    12001 cggcggtgac catggtgtcg agcaccttga tctcgcccga ctccttgtcg cggatcttgg
    12061 acacgacggg gatgatctcg ttctcgaagc ggccctcggc ggtcgcctgc tcggccagcg
    12121 cctgggagcg ggcaccgtag gcgtcgaggt cctcgcggga cagaccccac tcgttggcga
    12181 tgatctcggc cgacacgccc tggcccacca ggcccttgtg gccggcgatg agctcgacgt
    12241 ccgcgtagcg gtcgtaggcg gcgccgaacg ggaagccgac cccgctgccg atcgaggcac
    12301 ccatcggcac caggctcatc acctcgaccc cggcggcgat gaccacgtcg taggcgccgg
    12361 ccatgactcc ctgggccgcg aagtgggcgg cctgctggga cgagccgcac tggcgatcga
    12421 cggtggtgga ggggatctcc tcgggccagc cggcggcgag caccgcgttg cggccgacgt
    12481 tgagcgcctg ggcgccggcc tgcatgacgc aacccatgat gacgtcgtcg accagggcgg
    12541 ggtccaggtc gttgcgctgg gccagggcct tgagggtctc ggcggcgagg tcgaccgggt
    12601 gccagccgga cagggcgccg ttgcggcgac cgccgggcgt gcggacgatg tcgatgatca
    12661 ctgcgtcggg catgggtccc cctcggggag tcgggcggtc ggtgcgaact agaccggacg
    12721 gtcaagagag tctgctcgtc gacggcgtcg gggaccaagt cgcgggccgg gcctcgtgac
    12781 cccggtcacc gccactatcc tcggcggtcc gaaccaggag gcatcgcatg ggttccgtga
    12841 ccaaggccga catcgaccga ctcgtcgacg ggacgacggt ggcgagggag ttcgtcgaca
    12901 ccgtggcgcg ctgcggcgac tcgaccgctc tgcgctggat gcagctcgac gggacgttgg
    12961 gttcggtgag cttcgccgag ttcggcgacc gcgccgcccg cgccgcggcc ggactccgct
    13021 ccctcggcgt gcagcccggc gaccgggtcg tgctcatgat gcgcaacatc cccgagttcc
    13081 actggctcga catggccgtc ctgttgtgcg gcgccacccc ggtgtcgatc tacaactcgt
    13141 cgtcggccga ccaggtcgag tacctggtca accactgcgg ggccgtggtc gccatcgtgg
    13201 agaaccagga cttcctggcc aagttcgccg aggtgcggga ccggctgacg gcgctgcgca
    13261 ccatcgtgac cgtcgtcgac ccgcccgagc tccccgacgg cgtggtcggc cacgcggtcc
    13321 tgaccgacgc agatccggtg gacctggccg aggccgcgtc gatcggccgg cccgaggacc
    13381 tggccacgat catctacacc tccggcacca ccgggagccc caagggcgtg atgatcagca
    13441 actacaacgt ctgctgggtg ctcgagtccg gcctgatgtc gtacggctgg acccgcgagg
    13501 agatcaaggg caagcgggtg gtgtcctatc tgcccatggc ccacatcgcc gagcggatcg
    13561 tgtcgcagta ctccctggtg ctggccgggt tggaggtcac gacctgcccc gacacctccg
    13621 ccctgctgcc gcacctgatc gccgtgcggc cgaacatcgc cttcggcgtg ccccgggtgt
    13681 gggagaagct ctacgccggg gtgaccgccg cgctcgccgg cgaccccgaa aaggccgaga
    13741 agttcaacga ggccatcgcc gtcggcggcc cgatccggga gaagatgacc tggggcgagg
    13801 cgaccgacga ggagatcgcc acctacgagt tcctcgacgc cgtcgccttc agcacggttc
    13861 gcgagctcgt gggcatggac cagtgcgaga tggccatcac cggcgccgcc cccatcccgg
    13921 ccgacctgct gcggtggttc cgcaccatcg gcatcccgct cgccgaggtc tacggcatga
    13981 gcgagaacac cggcgccatg tccttcgagc ggttcaaggt caagcccgga accgtgggtc
    14041 tggccatgcc cggatccgag ctcgccgtgt tccccgacgg cgaggtgtgc tgccggggcg
    14101 gccacgtgtt ccaggggtac ctgaacgacc ccgacaagac cgccgaggcg atcgacgacg
    14161 acggctggct gcactccggc gacatcggcg agctcgacga cgacggctac ctgcgcatcg
    14221 tcgaccgcaa gaaggagctg atcatcaccg ccggcggcaa gaacatctcc ccggccaacc
    14281 tcgaggccga gctcaagtcc atcccgctgg tcgcccaggc agcggtcatc ggcgacaaca
    14341 ggccgttcgt gtcggcgttg ctggtgctcg acggagaggt cgcccccggc tgggcggccc
    14401 gccacggcat ccagtactcg tcgctcgagg agctcgccgc ccaccccgac gtgcaggccg
    14461 cggtggccga cggcgtcgag caggccatgg ccggcttcaa caacgccgag cgggtcaaga
    14521 agtggacgct gctctccgag gagtggctgc ccgacaccga cctgctcacc ccgaccttca
    14581 agctcaagcg ccggggcatc aatgcccact tcgagaagga gatcgaggcg ctctacagct
    14641 gagcggccag cccgggaccg ccgaccgctt cagcgtccga agtcggggtt gagcggcggc
    14701 aggccgagcg tgcccgccaa cgcgttcagg tcgagcccgt tcaggttcgc gatcgccacg
    14761 ccgatgagcc cggcgagcag tccgtagcgg gcgttcgggt tgttcgcgaa cacgtccgga
    14821 gccagcacct cggccagtcc cgggatcagc agggcgaacg tgcccagcgt ggacacgagc
    14881 acgaccgggt ccttggccgc cgcctgcaca accggggcga cgtcgggtcg accgaggatc
    14941 cccatcatcg ccgagagcgc cttgagcagg tcgggaccga ggaccttcga caacccgttc
    15001 agcgagctca cgacctgcgg ggtgagcccc gcggccaggc ccgagatcga gttcaggacg
    15061 aagttcacgt tcgggtccag gttggcggcg ttgccgagct gggcgaactt cgacgggtcg
    15121 gtgcgcatcg ccacgccgaa catcaccgcc aggagcgcca ggctggactt gtcgtcggcc
    15181 agacggccca gcccgttggg gtccgcgacc ttcaggacgc ttccgatcga cgcgacgacc
    15241 ttgggatcga cggtcgtggc cgccgagatc agcgacgcga tggcgagggg gtcgaccccc
    15301 ttgatggcca gagcggcggc cggatcgacc tgggctgcga gcatgaggat cgtcgtggcg
    15361 agcttgggat ccaggttcgc tcccgctccg agtcgccgga cgagggtggg atcgatgccg
    15421 cccagcgctc cggcgagcgc ccggatcgcg tcgggcgaga tgttcagcga cgccagggcg
    15481 gcggggtcca tgcccgtgag cgccgcgatc tgtgagatgt ccatgccggt cagctgagcc
    15541 agcagcgacg ggtcagcagc gaggcgggcg aacagctgcg ccgcggtctg ggccaggtcg
    15601 ccgctgcctg ccttggtgga tcgtgtggcc gagctcccgg ttcccgacgc cgagcgggcg
    15661 accgtggtcg aggtctgcag cgacaccttc tcgggggcgt cggtcgacac ctccgaggtc
    15721 tcggcggcgc gggcgcacga cgacgcagcg agcgcaagga cgacggtcgt cgccgtcacc
    15781 gctgcgagtg cgcgccgggt ccccatcgac aaaacgctac ggagcccggg tgctgcgggc
    15841 aaggcagcag ggagatcagc ccatccggtc gcgttcggga tcgaccttga cgaacctcgg
    15901 gtcgatctcg ccgggaccca gcggccgcat cagcaggtcg accgccgtcc acatcatgcg
    15961 cgacgacggg aagaacagca gcgggccgac gacggcgatc accacctcgg cggtgaggag
    16021 ggctccgtag ggtgggtcgg ggtagaacgc cagggtcccg gccatgagca cgacgagcat
    16081 gacgccgaac acgacggtgg tgttcagccc gagcgatccg atccagtggc cctcgacgcg
    16141 ctcgaacagg aacgtgcagg tggggcagcg ctcctccatc tggaaccacc ggcggaagac
    16201 gtgacgtcca ccgcaggccg ggcagcggtg gacgaaaccc cgcagcagga gcagcgaggg
    16261 ccggacgtcg agctggaccg tggtcgcgac ctccttgcga ggcacgcgac gttcgggcgg
    16321 ggaggccatt ggaaccatgt tggtcggcgc ctgccggccc cgtcacgccg acggcgcgcc
    16381 gaggaggacg tcgaggtcct cgaggtcgcg ctgggtggcg tcgggcagca accaggcggg
    16441 gaactgctga ggagacgccc cggcgagctt caccgtcgtc ccgccgtcga gctccagcac
    16501 gtacagccgc cacacgcccc gtcggaccct ggccgcggcg atcgtctcgg tggggacgcg
    16561 tcgcaccgtg cgccaggcca ccacgtcgcg caccaccgag ccgtcgacgt cgacgaagca
    16621 gcctcggacc gcgaccagcg cggcgacgcc gatcgcggcc ggcacggcga tggcggcgac
    16681 cgcggccaca ccgaccggtt ggtcggcccc gaacgcccag agggtcgacg ccagcgccag
    16741 cgacaggccg atcaccgcca cgacgaacag tggcagggcc agcacgctgg ctccacgcaa
    16801 ccggatgcga tcgggtcggg acatcggacg acgacggtag cgggagccct ccggcgaggt
    16861 gacggggacc gggcgcccta cggtggagga gtggcaggag ctgagccggc gcggatcctg
    16921 gtggtcgacg acgaccgcgc gatccgcgac tccctcgacc gcctgctcgc tctcgaggga
    16981 caccgggtga cgaccgccgc ggacggggcc tcggcgctgg atcggatgcg atccggctcc
    17041 gtcgacctgc tggtcctcga cctgatgatg ccgaccgtcg acggtctgac cgtctgtcgg
    17101 gtgctgcgag ccgagggcga ccgggtgccg atcctcatgc tgacggcgcg caccgagacc
    17161 tccgatcggg tcgcggggct cgacgccggc gcggatgact acctgcccaa gccgttcgac
    17221 cccgaggagc tgctggcccg ggtgcgggcg ctcctgcgtc gggcccgacc cgagcccgac
    17281 ggggagtccg accacgcggt gctgaccgtc gccgaccttc gactcgaccc ggcggcccgg
    17341 cgggcgtggc gcgccgaccg cgagctcgag ctgtcacgca ccgagttcga cctgctcgaa
    17401 ctgctcgtgt tcaacgccgg gatcgtcctc gaccggacca ggatctacga ggagatctgg
    17461 ggctacgact tcggtccgga ctcgaagaac ctcgccgtct acatcagcta cctgcggcgc
    17521 aagatcgacg agggtgagga cgtcaagctg atccacacgg tccgcggcgt gggctacacg
    17581 ttgcgggaga gctgacgggc ggagccgacg atgagcctga gggcccggtt cgtgctggcc
    17641 ctggtcggtc tggtcgccgt cgccaccgcc ctgatcggag cgttcagcta ccgcaccacc
    17701 cgggccgagc tgctcggcca gctcgactcg tcgttgcagg ccaccaccgc ggacctccgc
    17761 cgtccgggcg gcgtcgaccg ggtgctcggc ccgcggagcc cggcgtcgat ccgtggcgac
    17821 agcgacgtca tcgtccaggt ggtcggagcc gacggcaccg tctactcgat ggacggtgcc
    17881 gcgccgctgc cggtcggtgc ctccgccgag gacctcctcg acgagcccga ggggacgttc
    17941 cgctggcagg acgactcggt cgacggaacc gcggtccggg tgctgacgac ctccctgggc
    18001 ggcggccgcg gcgtgctgca ggtggggcgc agcctgagcg agacccgaca ggtcctggcc
    18061 gcgctgcgga cccggatcct ggtcgcgtcg ctggtggtgg tcgctgccgc agccgcggtc
    18121 ggagtggtgc tggcccgtca gctgacccgc cggctggtcc gcctgaccgc cgccgccgag
    18181 gaggtacgcc gatccggccg tctcgacgtc gacgtcgaca ccgacggcac cgacgaggcc
    18241 gcccggctgg gggtggcgtt ccgggagatg ctcggcgccc tggcccgatc ccgggaggac
    18301 cagcagcggc tggtgcagga cgccggccac gagctgcgaa ccccgctgac cagcctgcgc
    18361 acgaacttct acacgctgcg gcggtcgctc ggtccggccg gggtcggcgc cggggcagcg
    18421 ggtggggaca ccgctcggat cctcgacgac ctcgaccagg agaccgagga gctgagccgt
    18481 ctggtcgacg aggtcgtcga ggccgccacc gaccgtcggg gtgacgagcc cgagcagccg
    18541 gtcgatctcg gtgacctggt cgaacgggtg gcggagcgcg cccgacgccg atccggccgg
    18601 gaggtggtgg tgcaccgact gggtcccgcc acggtgtccg ggcgacccct ggcgctcgag
    18661 agggcggtga ccaacctggt cgagaacgct ctgaagttcg actccggcgt gcagccgatc
    18721 gaggtcacct gcgagggcgg ccgggtggag gtcgccgacc gcgggccggg gatcgcggcg
    18781 gccgaccggc cccacgtgtt cgaccggttc taccgctcca ccgacgcccg cagccggccg
    18841 ggatccggcc tcggcctgtc gatcgtcgcc gaggtggtcg ccggccacgg cggtcggacc
    18901 ttcgccgccg agcgcgccgg gggcggagcg gtggtcggct tcgtgctgcc ggtcgggccg
    18961 gaaccggccg gtcgaacggg cgcgacggcg tagtgtcgcc ggtcatgcag gaccgtgacc
    19021 cgcgggtgcg tctggagctg gacggccccg tcgccgtcat caccaacgac aaccccgaca
    19081 agcacaacgc gttcgacgac gccatggacg cgacgctgtt cgacatcctc gccgagctgt
    19141 cggcccgccc cgacatccga gccgtcgtgt ggcggggcga gggcaagtcg tggtcgtcgg
    19201 gccgcgacgt cggctcgatc gggatcaaca acaccgacat gacgcaccac gagctgatga
    19261 cccgcggcat ccgcggcatc cagcgcctgt gggacctcga agcgccgatc atcgtcgcca
    19321 tccacggctg ggcgctcggc gggtcgttcc agcgggcgct gctgtgcgac atccgcatcg
    19381 ccagcgacga cgcgcgcttc atgctgcccg aggtcggcca cggggtcatc cccgacaccg
    19441 gcggcgtgtc ggtcctctac gagatgtgcg gtcacggcct ggtgtccgac atggtgctga
    19501 ccggtcggcg tctgtcggcg caggaggcgt tgacccacgg catcgtgtcg cgcgtcgtca
    19561 cccgcgacga gctcgacgac accgcgatgg agatggcgca gaagatcgcg tcgagctcgg
    19621 cggtcgcggt gaagatggcg cggcgggtga tcggccacct cgcccgaccg gcggtgcggg
    19681 cgtcgatgga ggacgagctg atcttccaga cgttcgtcaa ccgcagcgac gacatggccg
    19741 agatgcgcgc cgcccgcgcc gagggccgcg agccgcgcta ccgcggcagc tgagccggag
    19801 cccggccgac cacccggagg acctctgatg accgaaccga tcggcctgcc ccgcccgcct
    19861 gctgtcggcg acacggcact tcgacccggc accttcgacg gcaccgcggt gttcgtcacc
    19921 ggcgccggca ccggcctggg caaggcgatc gcgtccgagt tcgcccggct gggagcggat
    19981 gtggtcatcg ccagccgcaa gcccgagcac ctcaccgccg gcgccgaggc catcgaggcg
    20041 atcggcgccc gggtgctgac cgtcagctgc gacatccgcg atgccgagtc ggtggccgca
    20101 gcgttcgacg ccgccgtcga cgccttcgga ctgccgggag tgctggtcaa caacgctgcg
    20161 gccaacttcc cggtcccggc cgaggacatg tcgcccaacg cgtggcgcac cgtggtcgac
    20221 atcaccctca acgggacctt cttgtgctgc cgcgagttcg ggcgccgtca cctcgcggcg
    20281 gggacgccgg gctcgatcgt caacatcggg gccagctacg cctggaccgg cgggcccggt
    20341 ttcgtgcact ccgccgcggc caaggccggg gtcaagaacc tgaccgagtc actcgcggtc
    20401 gagtggggcc cctacgggat ccaggtcaac tgcctggtgc cggggctgtt cccccacgag
    20461 gacatgaccg acgacatccg cggcaacctc gaccgcaccc acgacaagga cgcgtgccag
    20521 ccggcgttgc gggtcgggga gcgacaggag ctcgggtggg cggccacgtt cctggcgtcg
    20581 ccgtacggcc ggttcatctc gggtcacaca ctggtggtcg acggcgccaa ctggcagcgc
    20641 cgttcgatga cgaacccgcc ggtcaccacg atccgggacc agatgggccg ccccccgttc
    20701 gaaccctgac ccccccagtt ccccccggga ccccgctttg tagtgcgaga tccaggcgaa
    20761 cgcctggatc tcgcactaca gaacgggaat gtggtcacca gcgcgagcgg gactcctcgg
    20821 caaagccgag cacgtcctcg acgcgcagct ccgctccgtc gtcggtgacc acggtcccgg
    20881 tccaggtgcc gaacacctgg tggacctcgg tgccgagcac cttggcgtcg accttggtgt
    20941 gcttgtcgtg caccggtgtg agggtgatgt ccatgcgccc cgtcggatcg acgacccgcc
    21001 acggctcgag cggtcggtcc cagtcgtagt cccagcgcag ctccccgccg agcttgtgca
    21061 gacggccgtc gacgatgatc ccgttctcgg tgaagcccgt cccgtcggtc cacttgccac
    21121 cgatctggat gccgagcacc tcgccggtgg tggtggcgcc ggcgccacca ccccagttcc
    21181 agcgggtctc gtggggccag cggccacggc cgacgtcgag cacgccccac gcctcgccgg
    21241 cgtcaccacc gaaggcgatg acctcgtcgc cgatggtcag ggtgccgtgg gccggtcggg
    21301 cctggtgctt ggaggtgtac tggaacagct cgtcgctcca ggggatcacg acgttgagcg
    21361 actcgtggcc gtcgggcagc tcgacgaaca cgtccatcga cgcgggggtg ccgtcggcct
    21421 cggtccacgc ggccgacagg cgggtgccgc ccggctcgtc ggttaggtcg agcacctggt
    21481 ggcgggccgc ggagcgcagt ggcgcggtgc cgggccggtc cggcaaggag atccccgccg
    21541 ctcccgggac gctgcggttg cgtccgccgg tacgcccgga acgcaggtcg gcccaccaca
    21601 cgtcggtgat gcccaggaag tcgatgtcgg cgtaggtgac cgacagcgtg tgggtcggac
    21661 cgagcaccgc ccagtagtcc cagcgcttct tgcgcccggg ggtgccggcc aggttgcagt
    21721 cgtgcatcgg cacccgtgac cagccccgcg ccagcgggtt gagggtcgtg ccgcccggca
    21781 ggcagagctg gaccggctcg gtcacctccc gttcgtgggt gagggtcacg tcggggtggt
    21841 cgtcggtcat ggccggattc ttgtcggtcg ggacccgggc gcacgaccgg gcgtggaaac
    21901 ctgccggtcg tcacgagccg ccccgccgta gaatcggggc gtgctccgag cccgatcccg
    21961 gtccacgggg tcggcccggc cctcccgctc cccgggccgc cgtctgcggc ggttcggagc
    22021 cgagaccttc gcctcgctga aggtccggaa cttccggctg ttcttcgccg ggcagggcat
    22081 ctcgcaggtc ggcaactgga tgaccctgat cgcccagtcg ctgctggtgc tgtcgctgac
    22141 cgactcgggc atcgccttgg gactcctcgc cgtcgcccag ttcggtccca tcctggtgct
    22201 cggcccgtgg gccgggctgg tcgcggaccg gtccgacaag cggcggctcc tgctgatcgt
    22261 ccagtcgttg gcgatggtgc agtcgctcct gttggcggcg gtcgcgttca cggactcgcc
    22321 ggcctggacc gtctacgccg tcgcgctcat cggcggcttc accgtcgcgt tcgacaaccc
    22381 ggcccggcgc gccttcgtgg tcgagatggt gcccgaggcc gacgtgccca acgccgtcag
    22441 cctcaacagc gccctgatga cgtcgtcgcg ggtgatcgga ccggcgctcg cggggctgct
    22501 catcaccacc gcgggctacg gctgggcgtt cctgctcgac ggcgtctcct acgtggcggt
    22561 gctggtgtcg ctgttcctga tgcgcaccga ggagctgcgg ccgagcccgc cggcggagcg
    22621 ggccaagggc cagatccgcg aggggtttcg ctacgtgcgt cggaccgccg acctgttcgt
    22681 cccgctggtg atgatggggg tggtcggcac cctggcgttc aacttcggca cggtcatgcc
    22741 cctgttcgtc acccgcgacc tggacggctc cgacatggtg ttcaccgtcc tgttctcggt
    22801 ggtcagcgtc ggctcggtgc tcggagcgct ggtcgcggcc cgtcgtcggg aggtgacggt
    22861 gtggaacgtc gggatgtcgt cgctcgggtt cggggcgacg atggccgctc tggcggtcgt
    22921 gccgagcgtg gcgaccgcca tgcccgtcgc cctggtgctc ggcgtgttct cgatcacgtt
    22981 cctgaccatg tcgaccgcca tcgtccagat gcgcgccgag cccgagatga ggggtcgggt
    23041 gctcgctctg caggcgatgc tgttcctggg cagcactccg atcggcggcc cgatcgtcgg
    23101 ctgggtcgcc caggtgttcg gggcgcgctg gtcgatcgcg ttgggagcgg tcgcgtgcgt
    23161 ggctgccggc gggtggggga tggtgacggt cggtcgtggc gatcaccggg tcgggagcga
    23221 tccgggccgc gaggtgctca cgtcgtgagc gtgggaacgg tggtggcccg ccggtcggcg
    23281 tcgcacgccg agcgcacgtg gcaaccccgc aggtggtcgt tgaccacgcc gaccgactgc
    23341 atcgctgcgt aggcggtcgt gggcccgatg aagcggaacc cgaaccgctt gagctccttg
    23401 gccagcgccg tcgactgctc tgtccgggtg gcgatctcgc ccagctcgat cgggaccggc
    23461 cgtccccgtc gggccggctc gaaggaccag atcagcgcgg cgagcgagcc gtggcgcgcc
    23521 tgcacgtcga gcaccgcgga ggcgttcgcc accgccgcct cgatcttgcc ccggtatcgg
    23581 acgatgccgg catccgccat cagccgttcg acgtcgtcgg gcccgtacgc ggcgaccgcg
    23641 gcggggtcga agccggcgaa cgcggcgcgg aagttgtccc gcttgcgcag gatcgtcagc
    23701 cacgagagtc ccgactggaa cccctccagg cagagcttct cgtagagccg gacgtcgtcg
    23761 gtgaccggac gtccccactc gagatcgtgg taggcggcgt agtcctcggt caacgctccc
    23821 cacccgcagc gacggcggcc gtcggcgccg gtgatcaatc cggtctccac ggcgctggtc
    23881 gctcgtgtgt cagaccggcg tgcccggagc cgggggcgtg gtgccgacag tggggccggg
    23941 accccactcg ctgaccttgg tgctgtagcc ggcctgttgg ggcatggcgc cggtggcggt
    24001 cacgaactcg agctgctggc gggcgtagtc ggtcaacggg ttgacggtga cgccggttcc
    24061 gtacgcgcag atctcgctcc actggccgat cgccccggag tcgaagcgca tcccgatgat
    24121 ggccgtgctg cccagcttgc gggcctcctc gaccatgcgt gccatcgcct cgctgcggct
    24181 ctgggcgagc agcttggtga actcggggat ctcgccgcca ccgatggatc ggaacccggc
    24241 gatgcagccg gcgccgatgt tgcgggcccg gacggtcagg ccgaagatct cgccctggac
    24301 cgaggtgatg tcgtagccgg gcaggtcgaa gccggtggtg atgagcatgg gtcgcacgct
    24361 acgcgctgtg tcggtccgga cggagcggtc agcccaccga cggtcacttg accacctggc
    24421 agccgagctg gccgagcgcg ggtccgcttc cggtcccgac cgcgaccacg cagacctgca
    24481 cggtgtcggg tccgaccggg accgtggtgt ggaaaccgtg gctcgggccg tacccgaccg
    24541 cagccgccac atccagccgt tcccgatcgg cgggcaacga cgcgatcggt cgaccgtcgg
    24601 ccaggaccac gacggtgagg ggagcgacgg tgtcaccgtc cacggtccag ccggccagct
    24661 cgaccgcgga gggtgggccg ggcgacccgt ccggttcggt cgtgcgggtg accacggcca
    24721 tgtcgacgtt gccgaacggg ccgtccgggc tgagcagacc ggtgggcgtc accgggaccc
    24781 ggtgccgggc tcccgctccg ccgagctccc cgggggtggg ccagacgttg aacgagcagg
    24841 ttcccatggc cgccccaccc ttggactgct ggaccatgac cggcgcccgg acccacggtg
    24901 ccggacagct cgcatggccg aagcccatcc agtggccgac ctcgtggttg accacgtagt
    24961 ggcggtacgt ctcgaccccg tagggccagc tcggagaggc gcccagccac cggtcctggt
    25021 tgatgatgac gttgcgaccc tgccggcatg accacatgga gctgcacggc cgtccgaagc
    25081 cgggcacggt gtcggccgcg gccaaccaga cggtgaagtc cgaggggccg tcggtgcgca
    25141 cgaagcggat cgatcccccc agcgaccaac cccgtctgtc gtcgagggtc gcctggacgt
    25201 gtcgggcgaa ctcgccgaca tccgcggtga tccgcccccg ggtcgcgatg ttgaacgtgt
    25261 aggtgcgggc cggcgcgcag gcggtgagcc ccagtcccag caaggtcgac gtggccagtg
    25321 cggccagaac cgtccgcgtg cgacgtcgca tgatcccccc gatccccgac agcgacccaa
    25381 gggtattgcg gatggtcgtc ccggtgtcga tgcagtgatg cgaccggggt cagggcgtca
    25441 gggtgtcagg gtgtcaggtc gtcggggtga aggtgaggtg ctcggagacg tcgccccaac
    25501 gctcggggat catggtccgg tggacgaagc accagtcgcc gtcgatgcgg cggatccgat
    25561 cgtcgtagcg gccgacgacg accggctgga gccccagacg gtcggttccc tgtaggacgg
    25621 tgaaccggga ccggaccgcg atctcgtcgg ggtcgccggg gacctcatcg acgatgacgt
    25681 tggtgatcac gtgagcggtc agcggggttc cgtcgccgtg gcgccgtgtc gtcgactcgt
    25741 acagtgagcg gatctggtcg gcccccgtgg ctacgaccgt cccgtcctcg gttgccaccg
    25801 aggcgtgggc cagaagatcg ccgaccgcgg cgaagtcacc gctgtccatc agctcggcgt
    25861 agcgggcgag cagctcggct gcggcccatc ggtcatcggc ggtgatcgac acagcggaac
    25921 ctcctgggcc agatcgtcgc acgccgagat cacgttccgt tctggactct ccggtgttcg
    25981 cgtgtgagat cgacgtgtca ggcgtggttt ccagacgcga acgccaggca tcgggtgttc
    26041 gcgtgtgaga tcgacctgtc aggcgtcgct tccacacgcg aacgccgaat ggtccggcgc
    26101 cgatcggaaa tacgcgcgtt gatgcgcgcg tatttccgcg atcctgtctt accccttcat
    26161 tagggtggcg gcatgggtcg tccccgagtc tccgacgagc gacgcatcgc tacggcggtg
    26221 cgccttcccg agtccgtcca ccgtcggctg caggccgcgg cgcgggaccg ggacgtgtcg
    26281 gccaacctca tcgtcacccg ggcggtcgag gagtacctcg accgcctacc cagcgccgac
    26341 gcagtcctgg ccccctcccg caccggcacg ccgaggacgg catcgtgagc cccgcccgct
    26401 ccgcgagccc acggaacggc cggacgcgat cggcgacgtc gaccgccaac ttcggctcca
    26461 gccgtcgtga gagccacgac gcatcggcgt tctacgaccg gttcgtcgcc cccgagatct
    26521 cgaccgactc gacggtcaac ccgccgaccg agctcgatgt catctacaag accgacgccc
    26581 gccggatggc cgaggttcca tcggactctg tcgctctggt cgtcacgtcc cccccgtact
    26641 tcgccggcaa gcagtacgag gaggagctcg gcgtcggcgg ggtgcccgcc gactacttcg
    26701 agtacctcga gctgctgcac ggggtgttcg ccgagtgcaa gagggtgctt gaaccgggcg
    26761 gtcgcatcgc ggtgaacgtc gccaacctgg ggcggcggcc gtaccggtcc ctgtcgggtg
    26821 acgtcaccga gatcctccag gacctgggcc tgctgctccg gggtgaggtg gtgtggtgga
    26881 agggtcgcgc cgccggcggg tcctgcgcgt gggggacgtt ccaacgcccg gccaacccgg
    26941 tgcttcgcga catcaccgag cgggtcgtca tcgccagcaa gggccgcttc gaccgggccc
    27001 tcaccccggc ccaacgcctc gagcgcggcc tgccgtccac cgccaccatc tcccgtgacg
    27061 agttcatgga ggccaccacc gacctctggg agatctcgcc cgagagcgcc acccgcgtcg
    27121 gtcacccggc gccgttcccc gtcgagctgc cgcagcggct cgtcgagctc tacacctacg
    27181 agagcgacgt ggtgctcgac ccgttcatgg gttcgggcag cacggccgtc gccgcggtcc
    27241 ggacgggtcg ccacttcatc ggcttcgaca ccgaccccga gtacgtcacg atcgccgaga
    27301 gccgggtgtc cggcgaacgg gagcggatct cgaccgagcg gcccgatccg tcgggcgtcg
    27361 gcaccgtcgg gtccttccgg gtcgagctcc ccgccgcgcc ggtcgaggcg agctcgggcg
    27421 atgctccgga tccgttggcc gtcgcggtcc gtgacggtcg ccaggcacgc gaggtggccg
    27481 agatccttct gggtcagtgt gggttcgaca acatccgctc cgaggtcaag ccacgcggtc
    27541 tggggatcgt gctgagcttc gtggccgccg accggaccgg cgcggactgg gccttcgacg
    27601 tgtcggggac gttcaccacc aaccggtccg gcctccgtcg ggccgacacg ttgtggaagt
    27661 cgctcggcaa ggcggcggtg ctgcacgaga gccggacctc ggacgacggc gtggggatgc
    27721 cactcgttct gctgaccacc gatgctccgg ccaaggggac ctcgggtcac caggcgctga
    27781 gggtgctgcg aggtcaggac cggcctgtgt tcgacgtggt cgagctgctg gaccccgacg
    27841 gccacgcccg gctgcgttcg tacgcggaga acggtctctg accgaagtcg tgtccgggcg
    27901 gccccggcgg ctctggggcg tccgggccgt cgggctgtcg tagggtccgg gtggtgggca
    27961 gtgacgagac gcccggagcc gacccgatcg agacgacagg agcggagcac cgcaccgagc
    28021 gctggtcggc cgctgggctg tgggaccccg aggcgcccga cgcccaggtg cggggcgccc
    28081 tgctcgacta cctcgagtcg ctcggcgcga acgtggagcg gatggcccgg gccgaggccg
    28141 aaggcgagct cgtcgccctc ggcctcgaca tgttgctcga ggacggcggg ctcaccgtcg
    28201 agcagctggc gacacggatg ggagtccccg tccatgaggt cgtcgccgcc tatcgactcg
    28261 tgggagtgga ggtcaacgat ccggccgcgc cgatgttcga ggaggccgag atcgacttcg
    28321 tcgccctgct cacctctaca gcggggacgt tccccgacgc cgcaacccgg gagatcctcc
    28381 gcgcgctctc gacggcgttg tccgggttga gcgcggccgc gatctcggcg ttcgtcggca
    28441 gtgtcgaaga cgacctcgcc ggggccggcg atccggtcgc ccaggcgcgg gtgacccggg
    28501 aggcagggct gatgggcctc gatctggccg gagggctgcg accgctgctg cgccaccacc
    28561 tccgccaggg cgtccggcgg cagcgtgagg ggacacaccg cagcggcgat cgactgacga
    28621 tcgtcacggc ggtcggcttc gtcgacctgg tcggctacac cgcccgaacc accgacatgg
    28681 atcccgagga cctgatcggg ttcatcgggt cgttccggga gcggacccac gagatcgtcc
    28741 tggccggcgg cggacaggtg gtcaagcaca tcggtgacga gatcatgttc agcgccgtcg
    28801 acctggccgg ggtgtgtcgg atcgctctgg cgctgctcga cgggttcgac gagatcgaca
    28861 ccacgccgcg gggtggggtg gcggccggcc gagtcgtggc ccgccacggc gatctctacg
    28921 gaccggtggt caacctggcg tcacggctgg ctgacatcgc cgtcccgggt gaggtgctgg
    28981 ctccggcgga acgggtgacc agcgccgtcg agcctgacgc cggcgccgag gacctggtgt
    29041 tcgtccctgc gggccggcgt cagctcaagg gcttcgaccg accggtcgcg gtcgtcaccg
    29101 tcgaacgccc ctgctgacgc accgatctcg gggtcggaat cgacaacgtc tgtcggttcc
    29161 gaccccgaga atccggttcc gctcgtcgga tcccgtgggg caaggggcgg cgaaggctgc
    29221 accgaaccac ggcgcgtcgg ccggtgcgtc caacgctgaa ggctcgcgtc acccaccgcc
    29281 ggttgcgtga tcacaccagg tcgacggcgc atcgaagacc tggagcgccg gccagtcgtc
    29341 ggtcggccgt gacgagcggt acccctgccg cctcggcaag ggcgacgtag caggcgtcgt
    29401 acgcagtcac gttcggacgc agttcccaca cccgcggcag caggggcgag gtggggtaga
    29461 cggtgagtgg aaacgtcagc agatcatcca ttgcttggtc agcctgctgt ggggtcaggc
    29521 ccccgtgggc cgcgtgccgc cgcagcaccg acgcaacctc gacccggagc aggtcgggcg
    29581 cggccacgct ctcaccccga agtcgttcgc ggaaccgctg gccatcgctc ccgacgtcgg
    29641 ccacgactgg agcgagaacc gaagcgtcaa cgacgagcac gttcctcgtc gagtgcctcg
    29701 atcgcgtctc tcgccgacaa cgtgcccgtc gctcgtcgct ggattcggtc gaggacgtcc
    29761 tgcaccgtcg gcgtcgccgc gatctctgcg agctgggagg tgaggaactg ctggagcgac
    29821 tggccagctt cctccgcccg gcggaccaga gccgcatgga ctgcgtctgg cacatctcga
    29881 acctgcacgt tcggcatgca tgcatagtac atgcacgtgg gcatctcgtg tgcaagctcg
    29941 acgtagcgga tcgtggcaac aactgcccca gatgggagtc ccgtgtagcg tcggtgcggt
    30001 ggcatagtca tggcatggct cgcacacgtg tcagcaccac ggtcgatgac gaactcctca
    30061 caagggctcg ggaggtgacg ggcgcacact ccgactccgt gctgttcgac cgggcactcg
    30121 acgcgctcct ggcacaccat cgacgggccg agatcgacag tgcgtacgcc gcgtacgacg
    30181 aacatccgct cgacgaggcc gatgagtggg gcgatcttgc atcgttccgt tccgccgctg
    30241 ctgcgtcgtg actgaccatc cccggcgcgg cgaactctgg tggtgtgagc tgcctgagat
    30301 cggcagcagg ccggtcgtcg tgttgagtcg agatgtcgcc attccccgac tgggccgaac
    30361 cttgatcgcc ccttgcacga cgaccgtcag aggactgccg agcgaggtgg tgctcgagcc
    30421 cggggtcgac ccggttccgc gactgtgcgc cgtcaacctc gactcggtcg agagcgtgtc
    30481 cgtcggcgtc ctcgtcagcc gcctcggccg tctgggcgac gaacgcatgc tcgccgtctg
    30541 cgacgcgctc tgggttgccg tcgactgtca ctgagccggc gttctggggt ggtcagctgg
    30601 ccgtctcccg gcagtcgtcg acagtcggac ggttcgatcc agcagtgacc gctgactcgg
    30661 gacggcaccc gagcaggcga cgatcaccgg ggggttcatc gagcacgatg gacatcggct
    30721 cgaccaccag gtccgcggtg cactcgttgc cgaagtactc aacagtggcc cgaatcgtca
    30781 ccgaactgct ggtctcagcg acgtcccagt ccttgaactc cgagcaggaa ctaccgccga
    30841 actcggcccg gacgtcgacc tcccggccga cgggcggcga ggccaggctc cagtcgactt
    30901 cggcctcggt gcggtcctcg gcgcagcccg tcgcacacgc acagacggtc aacactgcga
    30961 caagtgcgag ccgcgtaacc atgtccacct cccacgagcg agggcaccgg aatccatcaa
    31021 ggagttcgag gcgtgttctc ggggtcgtag gcgccgcgcc actcgaccat ctcggcgtgc
    31081 tcggggtggg taggggcggt ggccgcggcg acgtactgct catggcccca cgtgcccccg
    31141 ctcgaccggg gcctgtcgag ggagtggccc gctccgatcg cggtgcgggg ccacgtcagg
    31201 aacaaggacg atcaggacac caacgtcatc cgcttccgcc gcgccgccga gtagggacgc
    31261 catccgtcct actcgcgcgg ggattgccta cttctcttgg tccggcttag aggccaagtc
    31321 ggaccatggc tgttccgtag tccgacttgt ggctgacata cggagaggtg aacaagtcga
    31381 tgagcctcta ctcgtagaac gcgtagggat tcaggatcgg gccctgcaac aagcccttcc
    31441 gttgcgcggt cgtgaggtgt gccgcgtcac acacctcgtc ccagtcgcgg cggatggtgg
    31501 caacctgttg gtcgccgatc tcgcgtcctt cttcgggagt caggtggtag atcggcgccg
    31561 cttcgaccaa cgcggcgatc cggctctcgc ggccggtgtc gccgtcgccg aagcgtgggt
    31621 ggcgggccac ctcgccagtg cgcggttgag gtgacacgtc gtaggcgggg gtgagcgcga
    31681 ggccgtcggc gaggatgaag gccgcgtggt tgcgggcgtg gtcgtcggtg ttgccgacga
    31741 ggatgttgaa catgatgcgc gagaacaact cccgcagggt cgcatcgggc tcgatgaagt
    31801 tcgctcggac ctcgtcagcc agctcgacgt aggagaagtg ctgcgcagcg acgatcgtct
    31861 gcatcgacag gatcgtggcc gctgacacga cgcggcgtcg ggcgcccgct ggcgatcggt
    31921 cgaagcgctc gaccagcagg accccgcggt ccatcgattc gacgaaccga acgggcgcga
    31981 cgtcgaggcc ggctcggcgt gcgagctcca tcgcgatgta ctccgactgg gtccacagga
    32041 agttgtcgct ccgccgcgag aacttggcca ggagcttcac accgtcgtcg tcgatcaagg
    32101 ccttcgggcg ggcgccgccg agcggagtgc cgttgacgag tgtcgcggcg aggtccgggt
    32161 cgatcgtctc gccttcgtcg atcttgtcgg ccgcggtcat gagctgctcg agcggagcat
    32221 gcaccggtgc tcgggggatg tactcggtcg ggctcgcttg gaagtcgatg gcgccgaccc
    32281 ggtctgagcc cgactcgagc aggtacacga gtggatcgag ctcggcgccg accgcgccca
    32341 ggttggcgtt gatgaccgat cgtccccacg agtccggtgc cgcgtcgtcg atgctcagcg
    32401 gcatcggacc gtcgagtccg tcgtgctcga agtcgtccag ggggagttcg ggctcgtaga
    32461 tggggaccgg gtccggtcgc tcgaggtagg aacgtccgta gctgaacacg tagcgggcgt
    32521 cgcgtcggtc caggcgtccg cataccaccg gctcggtggc gtcgggcaac cagatccaga
    32581 cgtaggcctc ggtcggcgcg tcagaagtca ttgcgcgcct tgcgtcgggc gcgaccgcgc
    32641 tgggggagca gcgccagttc gttgcgcacg acctggccgg cgaggtcctc gtttccgccg
    32701 aacagctcga cgccgaccag tcgggcggct tcgaacacgg tgccgatcgc cactgacggg
    32761 ttcccgcgtt cgatcttggt gatcgtcggg gcggaaaccc cgacgcgctc ggcgagcgtt
    32821 ccgacgctcc agccgcgccg tagccggccg gcacgcaccg aatcaccgag gatctggacg
    32881 gctgctcggg tccctcgtga ggtggccatg cgaagaatgc taggagttag acctcctaaa
    32941 tccaattatt ctttctactc gccgggaacg attgccgacg gaccgccccg cttgtgcctc
    33001 agtgaagtcg tagacgtagc ggaacttgtc gccttcggcg gccagcttgt cgagccggta
    33061 gcgggtctcg tcgagcacct ggctcatgtc ccagtcgggg tcagggactc cgtagtcgcg
    33121 accgccgatg cggaactcgt ggaggtggga gttccaccac ccgaacgccg cttggctcac
    33181 gcggtgcacc tcgtcgaggg tctcggaccc gtcgacgagc acgcggcgcc agatgggcgg
    33241 tgtgatgtcg cgcaacgtca ccttgacctg gaacaccttg ccgttggcgg cttgcttctt
    33301 ggtgccggcc ttgggggcat cgtgcgccgc catcttgggt gacatcggcc agctcacgtc
    33361 cgggcgttcc ggctcgcttg tcgagcgggc cgacgatgat gtcggacatc cgcatcgaca
    33421 aggcctcgag gtcgagatcg ccgttcgcca tgaagacgcc ttctcgcctc gcggtgtgcg
    33481 ctgtcgggac cgtagcgacg actgccgaat cgcgcactgc gtggtcgatt ccggcgccgc
    33541 cgacacccgc tccgacacgt tctcgattcg tgaggtcacg tgaaggtcac gccgggctct
    33601 cagcggccgg gtccgccgac ccggcaacgc tgtgagcggg acctttggag tggagctgat
    33661 gggactcgaa cccacgaccc cctgcttgca aagcagtcac ggcatggcgc gtcgaccctg
    33721 ccaggatgcg aacgcccagg tcacaggtcg gttctcgtgc cgcctggtcc cgtgctgtac
    33781 cggcctgtcg tggtgctctc gcggagtttt cgcggagtgc gatgtcctcc gcccaaagcg
    33841 tcggggagga acagcacgcc cgacagccgc cgctactgcg ccgccttggg cgggccgacg
    33901 agacggtcca cctcatcgcc cagctgcgac caaccgcagt aactccaagg cggcagagcg
    33961 tcgtcgccct gctgctgcag cccttgtgcg acacgctcga acccggcatc gtgccagtcc
    34021 gggtccttct tgcggaggcg agccactcgg taggtcattg gtcgatgtgg tccatcgtct
    34081 cggtagcggg gtcgtagacg tcgaagctgg cgatccttgg gcaaggcctg gcccttccaa
    34141 tcgagcagct cgatgactgg cgcggcacca actcgttgat cgtcgatcct cgccacgcga
    34201 agcagacagg tatcgccgtt cgatgccaca cgagcgagca ggtcaccggg ctgtaggtcg
    34261 gtcacatggc gccagggacg ccggacctgc cgtcgtgccg gctgggggcc ggtgagttga
    34321 gttctcaact tgctgagcgc ggcttcccgt ctcgccaaac cttgaggacc cgcttcctgc
    34381 cacggttcaa ggccttcacc acgatcgatg acactcaggg cgcgtgcttt gatctcatcg
    34441 tcgaggcggc ccaggctcgc ttgaaccgca gcgagcgcga gccacagcac gtgcacctcg
    34501 tcactgtcga ggtggcggta ggcggcaagg atccgaccgg ttgcctcatc atcagggacc
    34561 tggtcctcca acagctctcg gtagtccccg cggatatccg ctgccagatc atcggagaag
    34621 atccccggac cccatgcgcc catgtcggag gattgtcgca tgcacggacc gagggtgcac
    34681 tccggcgcgt cgtctactcc cgcaacgcgg ccatcgcgtg tgggagcgac gctgccagca
    34741 cttcgatgat gtcgtggctg gtcttggggt gtcggcgtcg tcgggcagcc atggcgtcga
    34801 tcgctccgag cacgccgatc gggtcgcgct ccacgtgtcc cacgacgaag gtgtcgacgt
    34861 cgatggcctc gatgccgcgg tccgcgagga gttcgggtgg gaagtcgcgg aggttctcgg
    34921 tgacgatcat cggtgcttcg acatggacgg caagggcgag gacgtggcgg tccttctcgt
    34981 tgaccggcat ctcctcgatc agcgaggccg gtggctcgtc cagggcggac ggcagggcct
    35041 ggttcatctg agcgatccgg cgatcgatcg ccgcagggtc gaggtccgcc cggagtttga
    35101 ggttgcgggt gacctcgtcg aggatctgtg gcgaccagtg cggtcggtac aggtgccgtg
    35161 tcgccatcgt gaggacgagg tcggtgacct cgatgccgta gagcacgttg gcgtcgagga
    35221 caacgacatg tgcggccatc gtgcccgggt gcccagcggg ctactggtag aggccgagct
    35281 cttcggcgtc ggctgtcatg gcgtcgagcg cctcgttggc ccgtgcttcg aggcggtcgc
    35341 ggtacgcgag aacatcgacg agcttcaggc gccgatgggt gccgaccatg tggtgtggaa
    35401 cttcaccggc ctcgatctgc ttgatcaggt atgggcggct cacgttcaac agctcggctg
    35461 cctggaccgt ggtcagctcg gcatgcagcg ggatgaccga cacgccgttg ccggctcgga
    35521 ggttctccac gatcgacacc agcagcgact ggatccgctc gggcagctcg atcgcgtcgc
    35581 cgtcgggtcc gaccagccgt ggaaggctgg tgccggttcc ttccagggcg cgcacgacgg
    35641 cttccagcac ctcggggccg agggcgtcgg gtcggacttc gagcacgtca ctcacggcga
    35701 caagcgtatc tacaaatgaa ataaacgcaa cgatgtatgc cgccgagccg atctgaccgt
    35761 tgttcggcca gactcgtatc ggtgatcacg acggaccagg agtatgacca gtcgcgggag
    35821 aagctcgcgt cgctggtcgg ttcgatcgcc agcaccgaag cgggcgccgc aggcgacgat
    35881 gagttccgtg acctgcaact cagcggactg cgtagcttcg ccgacgacct gcggaccgag
    35941 atcgccgact acgagacaca acagaagcgg cgttgacgtt ggccgaagtt cgcatcgccg
    36001 gtgaggaggg ccgggcactg gttctccagc acccacagct cgacggagat ggcggctggc
    36061 gttacaccgc caccctggag ttcgagacgg gccgagcgag cgtcgaagtc tgggactacg
    36121 gagacccacg tttgccggag gttttccgcc aggctgcgga ccagtggcga ggcttccagg
    36181 gtgccatcga gttcgcctcg atcgaggggc agctcgagat ggcgctgacg cacgacggaa
    36241 agggaaccat cagctgtgtc gcgaccgtgc gccagccgtg gccaccgacg tggcgaacca
    36301 caatcgagct ctcgttcggc gcaggtgctc acgccgagca gatcgcccgg gatgtcgagg
    36361 cgtttttcgc tagagcggcg ccggcatagc cggaggaagc gcgctactcc ggcccagaac
    36421 ctggcacgta actgatccag tccagcagtg gagcgcgttg ctctaggtcg agcctgctga
    36481 gcgccgctcg cgccagcaac cgaactgtct cccacccttc atcgtgctct accgcctcgg
    36541 tggtccacca agcggcgtgt tcctcgccag aacgctcctc gaagaacacc gagatcgcgt
    36601 cgaggacctc gcatcgggct gcttcctcaa cccgatgacc ggccacgagc cggtccaagg
    36661 tgatctggac gtcagaccag gtgagtgcca gctcgtcggc gaccgccacg tagtccggca
    36721 acgccgcgac ctgcccgtct gggtccagag ccaggaggcg cagcgtctcc gccagcgcgt
    36781 gctctgcgaa gtccaactcg tcaaccacga accgattctc ccaccgtcga cctcggccac
    36841 ccgcccaagt tcctcgcgaa gtttcgggct ctcgatgggc ctacgtaggt atggagggac
    36901 agacgagtgc attcggtcga acgggaggtc agcctggtcg cccgccttct gatcgacgcc
    36961 gtgcgggctg gtggtggccg cggtgatgcg ggtgacgacg ctgaaggcgt cggccgatcg
    37021 gttggatggg ctctcgtatt acgcgggcct ggccgaggac cggggtcgtc cgggtctggg
    37081 tcgtggtccg gtggactact acctcgatcc ggacgaacca ccgggtcgtt ggtgggggag
    37141 cggtcggggt gcgctcggcc tcgatggcgt ggttgccggg gaggatctgc gggtcgtgct
    37201 cgaaggtcgt catcctgggt cgggggcggg tctgggccgc cggttcggcg atacctctgc
    37261 ccgtgggttt gatgcgacgt tctcggcacc gaagtcggtg tcggtgctgt gggcgctcag
    37321 tccggattcg tgggtgcgtg ccgaggtcct cgccgcgcat gacgccgcgg tcgacgcggc
    37381 cctcggctgg ttcgagtgtc acggtgcggt gactcggcgg gggaccgacg gggtgttcca
    37441 ggtcgacacc cagggcctca ctgctgcgtt gttccgccag cacacctcgc gcacgatcga
    37501 tccgcagttg cacacccatg cggtcatctc ggcgaaggtg caggacccga ccggccgctg
    37561 gctctcactg gatgcacggt tcttgaagta ccagcagcgg acgatcggct ggatctacga
    37621 cgccgcgctg cgtgccgagc tgacgaaccg tctcggtgtc acctgggccg aacccggcga
    37681 cggtgcccga gacctcacct gcatccccgc cgaggtgaac gcggagttct cgacccgcac
    37741 gacccaggtc gacgcgaagc tcaccgagct ggttcgccgt tggagctacg accacgacgg
    37801 cgccgacccg gatgcccgga cgatcgcggg gttgcagcgt gctgcggtgg tcgcttcacg
    37861 ccctgccaag gggcacgggt tcgatccgtc ggtgctgcgg ctgggctggg aggaacacgc
    37921 acgggcgatc ggcttcgatc ccgaccacct cactgccgat gagctgaccg cccggcaacc
    37981 agaggcgtct cgtgtgccgg acgaggatct gatcgacgaa gcgctcatgc gggtgagcga
    38041 ggagtcctcg acatggctgc gcgccgatct ggcacggcac ctcgccactc tcctcgaccc
    38101 ccgtgctggg gccaacggtg ctgcgttggt cgccgaagtc gaccggctcg ccgagcttgc
    38161 cgaacggctc tgtgccccgc tcgggccgga acccgacggc gcgccgatgc tgcgcgccga
    38221 tgaccgtccg gtcaccgagc acgtcaccga ccggcgtttc agcacccacc gcatcctccg
    38281 cgaagaacag gacctgcaga cctgggccga gaccaacact cggcacatcc cgctgaacgg
    38341 tgacgctcgg cgcgacgccg ccgaagcgat cgccggtcat gctccattgg ttgtcctcgt
    38401 gggaccggcc ggtaccggca agacccgcac caccgcgacc gcggtcgccg agttgcagga
    38461 gctcggccgt ccggtcatcg ggcttgcccc ttcaggcaag gccgccgacg tgctgtgtac
    38521 cgaggcggac tgttcgactg acacgctcgc cgggttcctc acccgtcacc gacaccgccg
    38581 cacgaagtgg cctgcgggca ccacggtcat cctcgacgaa gccgggatga ccgccaccgc
    38641 ggacctcgcc caactcgtcc accttgttcg tgtgaaccgg tggcgtctcg ttgccgtcgg
    38701 tgaccccgaa cagctcccct cggttgcccg aggcggggtg ttcgctcact ggtgcaacac
    38761 cgtggcacac cacaccctcg acacgccgag acggttcagc gagccctggg aagccaccgc
    38821 cagcctcgga ctgcgcgcag gacaccccgc agcagtccag ctctacacgg atcaccgccg
    38881 gctgcacacc gcccacccgg cgctcgtagc cggccaggtc gcccgcgcgc accaacacca
    38941 caccgcatcg ggccggtccg tcgcgatcac caccaacacc gccgagaccg ccacagcgat
    39001 caaccaggag atccaacacc tcacccgccg gacgggcagc cccggggtga tgctgcacga
    39061 cgggaccacg gcgaggcccg gcgatcggat cgccacccgc cgcaacgacc cccgcctgcg
    39121 gaccgatggc ggtgagcgag tccggaaccg gcacacctgg accgtccagg ctgtccacgc
    39181 cggcggagcc atcgtggcga ccgacgacca gcgaggaacc gtgaccctcc ccgccgacta
    39241 cgtgaacgcg cacgtcgagc tcggctgggc cgtgaccgga tacggcaacc aaggcgacac
    39301 cgtcgacatc ggcatcgccg tgctggagcc gaccagcagc cggaaccacg cctacgtggc
    39361 catgacccga ggtcgcgagg cgaaccacgc cctcatcctc gaccggaccg ggaccacgga
    39421 tccggcagag cgcctggccg agatcatcac ccgaccaacc cgagcccact ccgcgctcgc
    39481 cacccaacaa cgcctccacc gagacgctgg agtcgaaccg ccgacgctcg acgacatggc
    39541 cgaaccgccg aagacgaccg tgtccggaga gcccccgacg gtggcccccg agctcgacga
    39601 gaaggtcgcc gccatcacga gacgactcga cgccctccaa cgacgggcac cgagccgaga
    39661 cgggcaggga ctcggcctgt agcggtcctc gacagtcccg ccttggcctg ccacgatggc
    39721 cgcatcgctc gtcgacggcg tcatgtcggc ctctacgttc ggacgccgcg cgtccgtgtc
    39781 ctggaaggcc ccgccgatgg cgatcaagaa gctccccaat ggacgctggg aagcgtccta
    39841 tcgcgacccc gccggacgag aacgcgtcaa gcaccaccgc acccgcatcg acgccgaccg
    39901 gtggctcacc accgtcaagg aacagctcca ccgtggcgac tacgtggacc cgcgccttgg
    39961 aaggcgacgt ttcgcctcgt tcgccgagga ctggctcgcc gctgccaagg cacacgtccg
    40021 accgaagacc tgggcgcact acgagtcgat cctccgtgtc cacgtcctgc ccacgttcgc
    40081 tgaccagccc gtcggtgcca tcagccccgc cgacgttcgt cgctacatca ccgacagcgt
    40141 cgaggccggc tccgctcccg gcaccgtgcg ttcggcccgc aaggtgcttc gcctcgtctt
    40201 ggctaccgcc caagccgacg gcgcgatccg agccaacccg tgcgacgggt cgcgcgtccc
    40261 cgcttccccg aaggccgaga tggtgttcct cagcgtcgaa caggtcgaga ggctcgcgac
    40321 ggccatcgac gagcgctacg ccacccttgt ccgtctcgcg gccttcaccg gtctgcgcgc
    40381 cggggagatc tgcgcacttc gagtgggtcg tgtcgatctc gtcagcggcc ggatcaccgt
    40441 cgccgagtcc gtcaccgagg tacagggtct cgggcttcac ttcagcgagc ccaagaccta
    40501 cgagcgccga tcggtgaccc ttcccgcctt cctcgccgat gagctcgccg ctcacctcga
    40561 ggaccggcct ggcgatcccg gcaccctcgt gttcacttca cccgagggct cgaccccgaa
    40621 ccacaagaac ttctaccgcc gccacttcaa gcctgccgtc tccgttgcgg accttccggc
    40681 cgagaccagg ttccacgatc tccggcacac ctgcgccgcc ctctgcatcg cgctcggcgc
    40741 ccaccccaag gccatccagg agcgtctcgg ccactcctcc atcaccgtca ccctcgatcg
    40801 ctacggccac ctcttcccca agctggacga gaccctcacc gaacgcctcg acgacctcca
    40861 ctgggctgtc gtcgagcggc gctgatcttc agcaaccgat catccgcact ggaggaccag
    40921 ccacgcctcg tcgcaggcag cggcgaccgg gagtgtccgg cggaacgtga agtacagagt
    40981 gccgacgtcg ccccacatcc atccagcatc gtcgtccgtg tcgagctgga ggaggagccg
    41041 ccagtccgcc tcacggtcga cgcggaaggt cggttcagcg tgacgccact gctcggacgt
    41101 accgagcggc aagccgcggg acacgacatc gcactctcgc cagatcgggc cctgctgcag
    41161 gcgcggccac ccgccgatct ggtggttggg actcgactcg acatcaacga cctcgttcca
    41221 gtcatcctgc atcgggaaga acctctgccg ggccgcctcc gccgccggtg ttcggtcctg
    41281 atgcggcggg aagaccgact cgaccgccgg ttcctcccag cccggaatcg acagcgtctg
    41341 ctgtggcgca agaccgatcg aaacgaacgt ttccaccccg tcaggagcac ctacggctcg
    41401 cgcatcgtcc gggtccacga ggatcactcg ccacccacct cgatcctcag gctcgaaccc
    41461 ccacggctgc tcttcgtcgt cgtagaagaa gttgaggacg ccctgcgacg ggaggcccgg
    41521 ctcggtgtcg aactgcgaca gttcagccag gtcgatgact gcgacaagcg acagtggtcg
    41581 atcgttccaa cgaggccact ggttggagtc gacgagaagc gcgccaccac ccagatgcga
    41641 ccgagtcgga tcgtccgtgt gatgcatgcg gaccgccgga cgggcgagcg actccaccac
    41701 cgctgccgcc tcggggttca gctgctccct gcagatatgg gagaggcgcg cccggagagc
    41761 cccggtctca gggttcatca cccgatccat tctctgcctg ctgtccctcg atcccgaact
    41821 tctccacgac gaagcgccag aacagaggca cggcctccgc cgccgagcgt tcgacgcgat
    41881 cgtttctccc gctgtgcgag aggtgctcga tcactacgtc gccgttgtcg gctggattca
    41941 gggaacccac tggcgctgag acccgaagga cctcgagcgg cggatcggca atgggccgat
    42001 ctgtcaccag caggtcgtgc atcgacgtca tggctgcgag gagctctctg ctcagggacg
    42061 cttcaacgct cttcacgatc tccaccatgt acccgaacgc gggctcccct gacgcgaggt
    42121 cctcgtatcg ctctcgaagt tcggaccacg gttggctctt gaactggtga cgccaggcca
    42181 acgacttcac agcggccccc tctcggacga cgtcaggacc cctcgatgct ctcaccttgt
    42241 gccactgcgt atcgcctctt taccacccgg tcctcgcggg gttttcgcgg agtcgaggcc
    42301 ggtgggtggg tcggccgatg gctgaccgtg ggtgcagatc ccttgggggg caagggatcg
    42361 gagagtggag ctgatgggac tcgaacccac gaccccctgc ttgcaaagca ggtgctctag
    42421 ccagctgagc tacagccccg gagcggagca ctctaaccgg gatcggggcg gcgctccgtt
    42481 ccgtcgctcg acgcagcatc acgtcgagca gccgacggga gctcggggtc gaccccgcct
    42541 cagcgagtcg gggcggtcat caaccagggg tcgttgatgt cgaggtaccg gtccaccttc
    42601 atgaccgact gccactcggt gaacatctcg gtggcgccct cgggcatcgg ggcggcctcg
    42661 ccgtcccggg cggccccttc ggtggtgaag tacgccacct cggtgaactc gtcgtcgccg
    42721 tagtaggcgg tcagcgcccc caggaggtcg ggccgcagtt gggtgagcat gtcgtcggag
    42781 tcccgctcga gtgctttggc gcgggcttcg tcggtcacgt ggccgtccat cacctgcacg
    42841 aaccgggcgc tgtcgacgcc gccgtgggtc atcaggtcca cgtcctcggt gtcgtggaag
    42901 gtgacgggac cgtcgaagca cttctcggtc tcggcccacc aggcaccctg ctcgggccgg
    42961 tcgctgttgc gtcgggccga ctcgcggtcc tcgaaccggg cgatcaggat gcagtcaccg
    43021 tccgacgtgc atccgccggt cgatcccagg tagccgacgg cgccgggcat caactccgcc
    43081 tcccagacct cgagtcgccg gtgcagcgcc tcgggatccg acgtcttgcc gtgcatcact
    43141 tgtacgaaca tcgggccacc tcctcggttg ctggtgggcc acccctcacg ctccgcccgt
    43201 gtcgcgaccg ggtcaagggc ggaggcgggt gaatgtccga tccgccatcc cgacgaccgg
    43261 gggtcgacct ggtcgacgcc gggggaggat catcgagaga ggaggacgcc gtggacgcca
    43321 ccgaactgct caccgacgcg atcgaacgga tccgacacgg cgtccacgag gtggtcgcgg
    43381 acctcgaccc cgagcagctc tccgcccggg tggcgccgga cgcgaacccg atcggttggc
    43441 tgctgtggca tctgacccgc gtgcaggacg cccacgtggc ggagttcctc gacgccgctc
    43501 agccatacct cgacggaggg tgggccgccg agctcgggct cgacccggat ccgtcggact
    43561 cgggctacgg gcacagcgcg gcgcaggtcg cacgggtgcg gatcgaacgt ccgcaacggc
    43621 tgatcgccta ccacgacgcg gtcgcggccc ggtcccttga gctgctctcc ggcctgagtg
    43681 accacgatct cgaccggatc gtcgacgagt cgtgggatcc gccggtgacc gccggggtgc
    43741 ggtgggtgag catcgtcgag gacagcatcg cccacctggg tcaagcctcc tacgcccggg
    43801 ggatcctgga ggcgcagtcc gcgggcgccg gctgaatccg ggctcggatc ctcgtccggg
    43861 cccggcggtt cggccggggt gtggtagtcc gtcaggccat gtccgagcag cggcgcgagc
    43921 gacgcaccga acggggcctt gatcggctcg tcgccttcag cgacggcgtc gtcgcgatcg
    43981 cgatcaccct gctggtcctg ccgctgctcg acgtggccga cgacgtctcc tcgtcggacg
    44041 tgtggagcga tgcctggccc cgcatgcagg gcttcgccat cacgttcgcg gtcgtggcca
    44101 gcttctgggt cgtgcaccat cggctgttcg agttgatcga cgactacaac ggtcggctgg
    44161 tctgggcgaa catgctctgg ctcgccacca tcgcgttcct cccgttctcc tccgaggtgg
    44221 tcggcaccgg ggacctcgac gacatgaccg tccgggcgct ctacatcgga tcgctggtgg
    44281 cgtgcagcgt ggcgctggcg gtgatcgacg tcgccatcct ccgctctccc gacacgtggc
    44341 ccgacggcga gcgccccgac atcgacgtga cagcggtcgt ggtcaggctc ggcgtgatgg
    44401 ccgccgcgtt cgtgatcggc accttcgtcg ctcccatcgg gctgtgggcc cttctcctgt
    44461 tgttcctggc ccgtcccatc accggttgga tcgagcgggg tcggtccgag ccggcgacct
    44521 gaggcgaccc ggcgccggtg tcgcggagcg gcaggtggcg ctcaggtccc ggcgtccggg
    44581 acgggttcgg gagcggcccc gattcggcgg atgctgcgag cggtcagcgc gaggacgacc
    44641 gcgacgatca cggtgctggt ctcgaggtcg taggagtcca ctcccagcag caccgcctcg
    44701 gcgatcacga gcgcggcggc ggcccacagg aagacccgga gccgctggcg ggccagctcc
    44761 cggggggtcc cgccgacgag gccgatgagc ccgatcgcca gcaggatcgc cgcgacgggg
    44821 atcacgatgt actggatcga cccgaactcg aactggcccg tatcggtctc gtcggcctcg
    44881 gccaccaggg tgtgggccag ggcgaccgcg gcggcgacga gcgacaggtg catcgggaag
    44941 tgcaggtaca cccaggccat ccggcgaccg tgacgttctg acagccccga ggcggggacg
    45001 tcggtgaagt agagccacca gatgccgaac accaccgcgg tggccagcgg cagcgcgaac
    45061 aggtcgacct ggtcgagcgg ctcgtgggtt gcggtgatgc cgagcttcac gaacacctcg
    45121 ccgagcatga tgatcgtgaa ctcgccgaag cggtggacgg tgtggtgcga gtccatcggc
    45181 aggcggcgct gctcggagcg ggtcggggcg acgaacagca gcaggcccgc gagccacagc
    45241 agcgggtacc accacggcca gccgatcgcg gccgtcgcca cgaacacggc ggccgacagc
    45301 aggcaccgca gtccgtagcc gcggtaggcg acgtcgaggt cgggccgggc gcgacgggcg
    45361 gcacggaaga acaacgcgag ggtcaccagc accgcggcga acagcggacc gaccgcccgg
    45421 ctgttgtcct catgcgagta gtccgacgcg atggcgacgg tgaggaccag caccatctgg
    45481 gtgaccagca gtgtgcgggt ccacgtcgtg gtgaccagac cggtgcccag cagcagggtc
    45541 gtggtcagcc acgtgaacca gatcatcgag aacaccaggg cgagccagat caccccgtcg
    45601 aggccggcgt agttcgagta cgtggtcgac atcaccacga tggcggcgac gaacaccagg
    45661 tcgtagaaca gctcgagcca cgactgctcg ggggccgcgg ggatcagacg gggcggagcc
    45721 cccggtccgg cgggggcggg tgtcccgttc acggttcggt gcggagtcgg ggcggcggga
    45781 acacgaggcc gtcgcgggtg gcgacggtgc cggtgcgggc cagggagcgg tacgaacgct
    45841 ccgcgaaccg ccacttcccg ccccgacgca cgacgacgtc ctggtacagg ccgaacgcgg
    45901 tcgaccagca gccggcgtcg gggtcgtcgg gcccgtggcg tcggacctcg cacatgtgcg
    45961 tgcggatccg ggctcccgac ccgtcggcca ccgagccgtc accccgacgg cgtgctccgt
    46021 ccgggtcggt gctcagcacc ccgaggacgt tgagggcgac gaactcgaag aagtcgaacc
    46081 gctcgacggc tccggcgatg aacccgagga tctcgtcggg gccgtgcagc gtgcgcgccg
    46141 gtcggtcgat caggtccagg cgcagcgccg cgtccggctc gaacagctcg gccagccccg
    46201 tccagtcgcg acggctcacg ccgtccgcgt agcgggcgat cagccgctcg agctcccact
    46261 cgtcgagtcg ggggtcggtc actctccacc cccgccgacg gccgacgcgg tgggcgaggt
    46321 gagggtgccg tcgtcgccga gcgagaagaa cctccccgcc cgggtgccga agtagatgcg
    46381 gcccttccac accgtcgggg tcgactcgat gcaaccgccg atcgccacgt tccagatctc
    46441 ggtcggctgc gtgcgggggt cgctgacgtc gtagccgtgc agcaccccgt tgcagtcgcc
    46501 ctggatcagc acgtcgtcga ccacgaccgg cgactgccag gtgggaccgg ccagcttgaa
    46561 cgaccacagc tcggcgccgg tctgctggtc gagaccgacg accttgccgc tgttggccgg
    46621 ggcgattacc acacccttgt gcaacgccgg ggtggcccag aaccccgccg ggttggctcc
    46681 ccggtcgtcg tacttccaca ccagcgggtt ggtcgggttc ttcgggtcga gcttcatcag
    46741 ctgaccgacc tccgcggcgc gccggttgcc gcgttcgtac tcgaccgcga cgtacaggaa
    46801 gccctcggca tcgatgacga tcgacgcgtc ggtgtcgtcg ccggcccaga accgcagcgt
    46861 ctgggtgggt gtgcctccgg tgcgcagcgg gcccatgtcc cagcccatga cgagcccgcc
    46921 cgagttggcc cagtaggcgg tgttgcccga catcgacacc gagttctcga tcgagaactc
    46981 cttggcgcgg ttgccggcca ggtcgttgat gagctgctgg tcccagctcg gcgtgttgaa
    47041 caccagctcg gggtcgacgg tcaccttgcc cgccgcgtcg tagccccggt tgagcttcac
    47101 cacgtggaac tggctgttct cgccgccctc gaacaggaag tcgtccgcca cgatgccggc
    47161 gccgtcccag tcgttgttcc acagcgtcgg cgacaccgcg gtggccgaca gcttccagag
    47221 ctcctgggcg acgggacccc ggtcgatggc gatcacgtgg tagtagctgt cgcggctgcc
    47281 ggagtagacg agcgggtagc cgtcggggtc gacggtgacg gaccccttga tgatgtcgcc
    47341 ggtgggcagc ggcgggagga tgtcctcgcc ggtctcgccg tccatgaagt gcacggcctt
    47401 gtcgtaggcg ccgaacacga cccaggtgcg accctctcgc tcgaacaccg ccggttggcc
    47461 ggtccagccc gacccgcacc aggtcttggc ggtgtggccg accgggctcg acgagcacat
    47521 gccgctgtcc ttcgggtagt accagcgcac cttgggggcg gaggggacgt cgccgcgccc
    47581 gtagtaggtg cgcgtcgggt ttccccggaa cgtgaggctg ccgggcacct ttgccgacca
    47641 catgcgtccg gagctggcgg ggttgacgaa ctggtccgtc tgctctgccg gcacctgggt
    47701 caggtccttg ggcgtgtcct tgtcgttcgg atcgtccttg ccgccgctcc cgcccccgga
    47761 gccggaaccg gagccggtgc cggtcgagtc ggccgcgtcg gttcgcccgg ggtcggagcc
    47821 gtcgacgacc cggctcacca gcgcggcgac cccgagcagg acgaggaggg ccaggccggc
    47881 caccaggagg cgtcgccgcc gggcgacctg cggcgacggc ggggcgtagc ggtcggcgga
    47941 ccgggggcgc tggatccgcg ggatgggatc gtcggtgggc tccacgccgt ccatcatggc
    48001 cgcccggcgc cggatcccga gccaccggga cgggcccggg ggctcactgc tggcgcagct
    48061 ccccctcggc gaccagcttg tcgagcgtca gctccgggtg ctcgctctcc aggcgctcca
    48121 gccagtacac cgactcgaac accgcgagca gctctccgtc ggagcgctgc agcacgtcga
    48181 cgccctgcat ggcgcgcagc tcggcccgcg atgcctcatc ggtgcgacgg gccacgcgga
    48241 accgggtggg ggtgagctcg accggcgcgc cgaactcgtt gatcaggcgg tgggtggcga
    48301 cctcgaactg cagcgcgccg accgcggcga ggatcggggc ctgatcgccg aactccgggt
    48361 cgcgcagcac ctgcaccacg ccctcctcgt ccagctgctg gatgccggtt cggaactgct
    48421 tggacttgcc gacgtcggtg gtgcgggcca ccgcgaagtg ttcgggggcg aagctgggga
    48481 tcggcgggaa cgtgaccggc tcctgcacga agagcgtgtc gccgacgcgc acgtcgttgg
    48541 cgttgaccag cccgaccacg tcgccgggga aggcttcctc gagggtctcg cgctcctggc
    48601 cgaacaccga gtgggcgtac ttggtggcga acggcttgcc ggtccggccg tgggtgacga
    48661 ccatgccccg ttcgaaccga ccggagcaga cccgcaggaa ggcgatgcga tcgcggtggg
    48721 cccggtccat gttggcctgg accttgaaca cgaagcccga gaacggggat ccgagcggcc
    48781 gtggttcgcc ggcggcgtcg aggcgggcgg cgggcgcggg gacctgctcg atcaccgtgt
    48841 cgagcaggag ccggacaccg aagttggtca gcgcggagcc gaagagcatg ggggtggtct
    48901 cgccggccag gaacagctcg gcgtcgtgct cggtgccggc gagcagctcg agctcgtcgc
    48961 gggcggcgag ccaggcgtcg gcctcctcgg cctcggcccg gtcggggtcc accagctcct
    49021 cgggggcttc ggtcgcgccg cgggcggtgc gggtgaagcg gatgaagtcc ccgctcgagc
    49081 ggtcgacgac accacggaag tcgcccgcga tgccgaccgg ccaggtcatc ggggtgcaca
    49141 tgatcgacag gtgcgactcg atgtcgtcga tcagctccaa cgggctgaga cccggtcggt
    49201 cccacttgtt gacgaaggtg atcagcggca ggccgcggtc gcggcacacc tcgaacagct
    49261 tcagcgtctg cggttcgatg cccttggcgg cgtcgaggac catgaccgcg gcgtcgcacg
    49321 cggtgaggac ccggtaggtg tcctcggaga agtcgcggtg cccgggcgtg tcgagcaggt
    49381 tgaccacctg gccccggtac gggaactgca gcaccgtcga ggtgatcgag atgccgcgct
    49441 gctgctcgag ctccatccag tccgacgtgg ccgaccggcg gtcgccgcgg gccttgaccg
    49501 ccccggcctc gcgtccgagc gcgccgccgt agagcagcag cttctcggtc agcgtggtct
    49561 tgccggcgtc gggatgggag atgatggcga aggtgcgccg gtgggcggct tcgttcacgg
    49621 ggtccgcgga catgagggtg tcagttcacc acctggtcga ccgcggcgag gagtccgagg
    49681 gtgcgggcgt cgcccgcgat cgccagctgt gccgcgttca tgacgtacgc gccggcgacg
    49741 cggcggtccg ggtcggcgaa gcccagggaa ccgccggcgc cgtagtgtcc gaaggaccgg
    49801 ccgtcgagca gcggcgacag cggcgagtgg gtcatgaacc cgagagcgaa cgggatgtcg
    49861 agcagcggca ccgtgctggg tccgctgacc tggggggcga tcgcccgctc cagcgtgtcg
    49921 gcgtcgagca ggcggacgcc gtcgacctcc gacaccagcg ccgcgtacat gcgggccagc
    49981 gacgccgcgt tggtgacgcc gttggcggcg gggatctgcg ccgatcgcac ccgggcctcg
    50041 ttccacacct ggtcgtcggc gaacgcgccg ccgggagcgg tcaacgcccg gccgagcagg
    50101 ttcccctcgc cgagcagcat gtcgagcatg ccgaccagcg acggcgcgtc ggtctggctg
    50161 tcggcatcgc ccgcgggttc ggcggcgggc agaccgggca ttccggagtt gatgagcggg
    50221 gagacccggt gctgctcccc gtcgggcagg ccgacccaga ggtcgagtcc gagaggttcg
    50281 gagatctcgc ggcggacgaa ctcgccgaag tcgacgccgg ccacccggcg gacgacctcg
    50341 ccgaccaacc acccgtaggt gacggcgtgg tagccgtagc cggttcccgg ctcccacagc
    50401 ggtgggctgt cggcgagggc gtcacagatc gtggtccagt cgagggcctc ctcgacggtc
    50461 atggcccggt cgacgtcgac caggccggcc tggtggctga gcagccagcg caccgggatg
    50521 tcggccttgc ccgcggcgga gaactcgggc cagtagtccg cgaccggcgc gtcgaggtcg
    50581 agttcgccgc gttgggcgag caggtgggcg caggtggcgg tgacgccctt ggtggacgag
    50641 aacaccagct ggagggtggt gtcgtcgtag gccgtgccgt cggtgtcgcg gacgccgccg
    50701 gtcaggtcga ccaccttggc gccgtcgacg tacaggcaga acccggcgcc gatgtcgccg
    50761 ttctcgacga agttcctctc gaacgcctcg cgaaccggtt cgtagccggc ctcgaccgac
    50821 cctctgatct ccgacatcgg cacctcaccg tcgatcgcgg gcgcaccggt cggtggccca
    50881 gcaccgacgc gacggtagcg gggtgtcggc tcagccgttg tcgacccgga accggctgac
    50941 tcgctggacc gtccgtccgt cggatccgac cgcctcgacc agcacagcgt gcggcccgtc
    51001 tttcagctcg gtggtgtcga agacgggacc ctccgcggtc ccctggtcgg acgacggcga
    51061 ggtggtgagg gtgaagggag cctggcggtc gacgtgttcg ggggggcgga cgtcgtcgag
    51121 gtggtcgacc cagaagccga ccctgacgac ggcggccgag tcgaccgacg gctcgaaggc
    51181 cacgaccacc cgtccgttga gggtggctcc ctcgagcggg tgagaggccg ggtcgctcgc
    51241 cgatcgcgac cagacgatcc ggtcgccggg tcgttccagc tgagtcggca gaacccgggt
    51301 gggcacggtg ccgggaacgc tcgacgcggg tggggtcgcc gtcgtcggcg ctgctgtgga
    51361 cgacggggcg acggtgctcg gagtgacggg agcggtggcc ggggtccccg acgatccgtc
    51421 gcgggccgac gccgcctggt aggcgagcgg caccgacacc gacgcggtgg ccaggaccga
    51481 cgcggcgagc gcgagcagca tccggccccg gggggtcggg gggtcgggtc gggacgagcg
    51541 cgagccgctc acctcaccgt ccccgttcgc catggcccga ttctgtccgg ccgggagccg
    51601 gcgcgacatg ggcaattcgc ctgtgcatcg tcctccctcc ggacggcgga cccggagccc
    51661 gcgtggtgct cgtctcgtcc gtcaggccgc ttctccgtcg tcgggtgccg agtggaggtc
    51721 gatgatcccc agtcgcacgg ccgacagcac ggcgtgggtc aggctctggg tgtcgagccg
    51781 gcggtaggtg gcgttgaggt ggttgtggac cgtcttctgc gtgatcccga gggcgcgggc
    51841 ggcctgcttg gtcgacatcc cgtcggcgat catctgcagg atctcgacct ggcggtccga
    51901 cagcaggtcg ttgcgcgtcg gctccggacc gaggtgctcg agcatggacg cggccagccg
    51961 ggggctgagt gtctcctcgc cctcctggac ccgtcgcagg gtgtcgagca catcggcgaa
    52021 cgaggttccc ttggtgaggt acgccgccgc gcccgcctgc agtgcggcgc gggtcatggc
    52081 ggggtcgtcg tgcatggtca gcacgacgac cttggagtcg ggaacctcct cgcggattcg
    52141 gcgggcggcg ccgaccccgt cgagcaccgg catcgacagg tccatcagca cgatgtcggg
    52201 ccgggtctcg atcgccaggg cgaccgcctc ctcgccgttg tcggcctcgc cgaccacgtc
    52261 ctctccggcg ctctcgagcc cgcggcggat gccgtcgcgc aggatctggt ggtcgtcggc
    52321 cagcagcacc ctcacgatgc cacctccctg ttggtgtcga tgctccgctc ggagcgagcc
    52381 gtgctccggc tgtccgtcgc atggctgtcg ttgatgtggc tgtcgttggt gtggctgtcg
    52441 ttcacgcgac ggaccggggt gtcggtcctg gcccggtcac gtgggggcgt ggggttcggg
    52501 ccggcggtca ccgtcaccgt ggtgccccga cccggggtcg agtcgatggt gagacgggcg
    52561 ccgaccacgt cggcccgttc gcgcatgccg acgaggccgt acgcggagtc gcgcacgccc
    52621 ctggcggact cgaagccccg cccgtcgtcg gtgacgacca gctcgaactc gctgccgcgc
    52681 acgtcccaga tcacgtccac gtgctccgcg tcggcatgtc ggtccacgtt cgtgagggct
    52741 tcctgaagga ttcgcagcag ctcgttctcg accgggaccg gggcgcggtc ctccgggtgc
    52801 acgacggtga gggtggcggc gacgtcggcg cgttccgcga accggttgac cacgtcgagg
    52861 gcgatcaccg acagaggttt ggcgtcggtc accccggagc ggagctggcg cagggtctcg
    52921 cgcagctcgt cgagcgcgga ctggacgtcg ccctggagtc gcatcaggtc agcggtggcg
    52981 gggtcgtcct cggccacgat gcgctcgagc tccatagaca cgtaggtgag ccactggccg
    53041 aggcggtcat gcaggtcgcg ggcgatgcgg atccgctcct cctgtgcgcc gagggtgcgg
    53101 agccggccga accagcgggc gttgtcgacg gtgagtgcga gcacctcggc gagccccgac
    53161 agcagcaccg cgtcgtggga gtcgtagcgg gactgcgacg gatgctccag gccgaggacg
    53221 ccgatgacct ggccccgggc ggtcaaccgg acgtacaggc cggaacggct ggtccggacg
    53281 atcggtcgga gcgcctcgga gccggggtcg aggtccccct cgcggatcac gccgtcgtgg
    53341 tcgagggctc gaccgagcgg cgccggcagg tcggaacgcg gataggcggg ccgcagagcg
    53401 cacccgtcgg cgatcttggg cacccactcc tcggagttgt cgtcgaaggt catcaggcag
    53461 atgacccggg cgtcgaagct gtcctggagc tgctggcgga tccgctccag ggcttcgcgc
    53521 agcgacagcg agtcggggag ggtgcgcgcc accacgttga gcatctcgag gagctcgttg
    53581 gtctccgaca ggtggtggac ctcgccgagg agtccgatgc gacggcgttc ggcgtccttg
    53641 atgcgggacc ggacgtagcc ggcgaccacg acggcgagga ccagggtgac gacagcggcg
    53701 acgtcgcgct ggtcggtcac catgtcgggg acggaccgcc ccccgaccgc gccggagacg
    53761 gcgacggaga ccacggccac gaccagggcg accccgccgg tgacgaaccc ccatccgagg
    53821 gcgacgaccg ccaccgcgat cagcaggcag aagtagaagg ggctggccca ctcgcgcccc
    53881 acgcccatgg cgacaccgaa caggacgacg tcgatgaagg gttcgatacg gcgcagggcg
    53941 tgcgtcgatc cgagctgcac cggcatcacc gtgcgccagg tcgtgacgaa cacgcacacg
    54001 gcggtggcga cgaccgcggc gtaggaccct cggaacgcgg cgggggccga gaagacgagg
    54061 ccgtagccga gcgcgcacca gcgcagaacc gcgatcgacg gagacaccga ggcgcgccac
    54121 gtcggcggca gtccgagcag ccgcgtggac tcgtccaggc caccgtgggg gagggcgcgc
    54181 atggcggcga cggcctccga cacgtccgcg gtcgacacgg acgggtgcgt gggcgctccc
    54241 gcggtgaccg gacccgaggg ggtgattgcc ggttccgtgc cgtcacccta tcgacggctc
    54301 cgcggaatcc ggcgggacct cgggaaccga atcgcgggcc gatgcccggg ccgggccccc
    54361 gacggacggc cggggggatg gggccggacc gggtggcggc tcctagtctc gacggccgtg
    54421 gccgaccccg acgagacgac gtcctatccc gaccggacgg tgtcccctcc cgaggaggcc
    54481 gtgaccttcc ccggagcgcg gcgcccggac cgacaccggc gggtcacggc gtccggcatc
    54541 gagctgcacg tgtgcgagtg gggtgacgct tcggcccctc cgttgctgct ggcccacggc
    54601 ggcttcgact tcaccgggac cttcgacgtc ttcgcgccga tgctggcgga cgccgggtgg
    54661 cgggtggtga gctgggacca gcgcggccac ggcgactcgg atcgcgccgc gctctacacc
    54721 tgggacgccg acgtccggga cgccgccgcg gtgctcgaca cgctcgggtc ggatcccctg
    54781 ccgttcatcg gtcactccaa gggcggggcg ctgatgctgc acctggccaa cgcgctgccg
    54841 caccgggtgt cgaccatggt caacctcgac gggctgccgt cgcaccaggc catgtccgac
    54901 gtctccgaca ccgagcgcac ccgtctgctg cgcgagcagc tcaccggctg gctcgacttc
    54961 cggcggtcgc tggtcggtcg acagcgtcga ccggggacca tcgacgagct ggccgaacgg
    55021 cggggccgga tgaacccgcg gatgacccac gactggttgc gctacctggt caccgtgggg
    55081 gcccgtcgcg acgacgacgg atggcgctgg aagatcgacc cgaccatgcg catgggcggg
    55141 ttcgggccgt ggcggccgca gtggtcgatg ctgcgcctgc ccggcatcgg cgtgccgttc
    55201 ctgggcgtcc tcggcctgga gctcgaggtg atgggatggg ggaccacgcc ggcggatgtg
    55261 atcccgaacc tgccgccggg gggtcgcctc gaggcgttgg aaggggtggg ccacttcgtc
    55321 cacatcgagc aaccgcggct ggtggccgac ctggtgctcg agcacctgac ggcgggtgcg
    55381 cccgcaggag gctggtcatg agcggcatcc gcaccctgcg tcacaaccgg atcgacctgg
    55441 ccctgcacga gctgcgcggc accgatcccg cccatcccgc cgcccggccc ctgctgctgc
    55501 tgcacggcct cggcgagcgc accccggacc gggtacccac cctcgtcgag tggaccgggc
    55561 ccgtgttcgg actggacttc accggtcacg ggtgctcgac cgtgccgacc ggaggcggct
    55621 actccgccga gatcctggtc ggtgacgtgg acgcggcgtt ggccgagttg ggtccggtca
    55681 ccgtgctggc ccgcggcctg ggctcctaca tcgccctgct ggccgcggcg gcccgtcccg
    55741 agctggtccg gggcgcggtg ctggccgacg gcccgggcct ggtcggcggc ggggtgcacc
    55801 cgggctcgcc gaccgtgccg tacgtgcccg aggagcgggt gccggccggt gaggccccct
    55861 catcaccgga cccgttcgct ctcgcggagc tggcgcggga ccctcggccg ccggactacg
    55921 tgcagacctt cgtccggttc gtgctcgagg gatcggacct ggacctgcct ctgtgggtgg
    55981 cggcggtggt ccgcccggag tggctcgcgg cggtggccga cgagccgggc gtcgggcagg
    56041 ggaccatcgg cgacggcctc gcccactacg cgtcggcggt ctgacccggt ccgaacgacg
    56101 cggccgcagc tccgccgcgg tggatcgcgc tggggagcgc gcgccgggga gcggtcagtc
    56161 catctgctcg aggatctcct cgacgcggaa ccgggtgccg tcgatgcggt cacgacacca
    56221 cgccaccagc gccccggccc gctcgacccg atcggcgagc acgtcgacgt cgagcgcgtc
    56281 ggactcgagc tcggcgacga tggcgtcgag ctcggccatg gcgccggcga atcccagctc
    56341 ctcggggtcg atcggtgcgg tgggctcaga catggggttc tcctcccgga tcggccccct
    56401 cggcccggac cgtggggtct ctggcggcgg gggtgatgcc gagcacgctc gaggtgaccg
    56461 tcccgtcggc cagcgtcgtg atcagctcct ggttcgtggc cagcgcgtcg acggaccgga
    56521 cgaggctgcc gtcggcgccg cgggtgatcg accacccgcg gcgcatcgcc aacgccgggt
    56581 ccagcgcagc ggtgctcacc gcgatcagct cggtccgcgc gtcggcgcgt tcgatccgtc
    56641 ggcgggctcc ggcgtggatg cgtctgacgc ggtgatcgag ctcgagggcg ctgcggtcca
    56701 gccgggcgac ggcgcgggca ccggtgcgct cccggatgcg gtcgagtcgg acctcggcgc
    56761 ggtcgagacc gccgtgggtt ccggcgcgag cggcggccca ccgatcgagg atccgtcgct
    56821 cggcgtcgcg cacggtccgg accagcagct gggccgccgc ggtcggagtc ttctgggcgg
    56881 tgtgggccac ctcgtcggcg acgctccggt cggtctcgtg accgatgccg gtgatgaccg
    56941 cgacgtcgct ggtggcgatg gcccgggcga cggcctcgtc gtcgaacgcg atcaggtccg
    57001 tggtcgctcc gccgccgcgc accagcagca ccacctcgac gccgtgggcg accgcggcgc
    57061 ggatcgcccg ggtgatcgag cccggggcgt ccgcaccctg ggtgcgggcg tcgaacacct
    57121 gcacgtggaa cccgatcccc gaggactcga gctcgtggag ggcatccgcg tgagcagcgc
    57181 tgccgacgct ggtgacgaga ccgacccgca gcgcgaccga cgggaggtgc acctcggcgt
    57241 tggcgcgcag cagcccttcg cgggtgaggg ccgcgatcaa ccgggcccgg tccgctccga
    57301 gcagccccag ggtgaacgcc gggtcgatcc ggtccatcag cagctgcagt gacgaccggg
    57361 ccgcatacgt gcccagccgt ccgccgatgc ggatgcgcag gccgtctgtc atgcggatcg
    57421 ggtcaccctg ggcctgcagg aaccggttga cctgctggcg ctggctgtcg aacagcgtca
    57481 ccgccaacga cgggcgcgcc gccggggacc gaggtgtgcc cgagtccgga tcgtcgggct
    57541 ccaccaggcg gaagtacacg tggccggccg cggagcgggt caggtcgacg atctcgccct
    57601 cgacccacac cacgtcgccg aagacgtggg cgagcacgtc gttgacggcg tcgtggagcg
    57661 caccgacgct ccaggtggcg gtctcgatgg gagctcagtc cttgccgtcg gccggctcgt
    57721 tgcggatgac gagcaccggg atgtgggagt ggtgcacgac gtactcggag atcgatccga
    57781 tcacgacgcg cttgaaccaa cccttgccgt gggagccgac cacgatcacg tcgaccccgg
    57841 actcctcggc aacgtgcacg atcatctgac ccgacgcgcc ctcgaccagc ttgcgctcca
    57901 cgatcgccgg atcgacgccc atcgcctcga tggtgtcggc gagctcgtcg tcgccctcgt
    57961 cgaggatggc ggcgcgggcc tgctcggcct cggagggcga cagcaggttc ccctcgaacc
    58021 cgccggcgcc cgagtcctcg gcgacgctgg tgtccgcgac ggtgagcagg gtcaccttgg
    58081 aggggttgag cagggcgacc gcccgtcggg ccgcctcgat cgagacatcg gagccgtcgg
    58141 tggcgatcag agcgtgcacg gagcctcccc gggtcccggg ccggtcccgg gtcggaccaa
    58201 ccctagagcc atcacccgcc cgttccgctc cgaaccggcc cggaaccagc cggcacgtcc
    58261 gtcgcggcgg cggtgtcggg agtgtcggga tcgctgccgc tgacgagcac ccggtagaga
    58321 tccgcgtaca gacccccctg ggcgagcagc tcgcggtggg tgccgcgctg cacgatccgg
    58381 ccgccgtcga cgaccacgat caggtccgct ccgaccacgg tcgacaaccg gtgggcgatg
    58441 acgaccgcgg tgcgcccctc gagggcatcg gccagagcgg cctggatggc ggcctcgttc
    58501 tcggtgtcga gcgccgaggt cgcctcgtcg agcaccacga tcgccgggtc cttgaggatg
    58561 aggcgggcga tcgccaaccg ctgctgctcg ccgcccgaga gccggtagcc gcgctcgccg
    58621 accatcgtgt cgaagccgtc gggcatggcg tcgaccacgt cgagcacccg ggcggcccga
    58681 cacgcctcgc gcagctgctc atcggtggcg tcgggccgcg cgtagcgcag gttctcgccc
    58741 accgtcgcgt ggaacaggtg cgggtcctgg gacaccaccc cgatggcccg ccgcacgctg
    58801 gcctgggtca ggtcccgcac gtcgtggccg tcgaggagca ccgagccctc ggtggcgtcg
    58861 tacaggcgcg ggatcagcga cgcgatcgtc gacttgccgg cccccgacgg gccgacgagc
    58921 gccaccgtcg tccccgcagc gatgtcgagg tcgatgtcgt gcagcaccag gggcgggccg
    58981 tcggggtgct cggtctccac cgccccggtc tcgagcgaac ggatggagcc ctcgtcggct
    59041 ccggggtagc ggaaccagac gtgctcgaac cggatggagc cggtggggtc ggtcagctcg
    59101 gcggcaccgg gccggtcggc caacgggttg ggcgagtcga gcacctcgaa gacccgctcg
    59161 aacgacacga acgcgctcat cacgtcgaca cgggcgttgg tcaggctggt cagcgggtcg
    59221 tagatccggg tgacgagcgc ggtgagcgcg acgagcgtcc cgatggtgat gttgccgtcg
    59281 atcacctgca gtccgcccag caggtacacc gccgcggtgc cgatggcgcc gaccagcccc
    59341 atcatcacca tgaaccaccg cgagtacatc gccgagcgga tgccgatgtc gcggacggcg
    59401 acggcccggg tggcgaactc gtcggtctcg cggtcgtggt ctccgaacag cttgaccagc
    59461 tgggctcccg acaccccgaa ccgctcggtc atctgggcgt tcatgccggc gttgagctgc
    59521 atcgagtcgc gggtcatcga ctggagtcgg cgtccgaccc gcttggacgg caccaggaac
    59581 aacggcagca gcacgacggc gaccagcgtg atgcgccagt cgagggcgat catcgccacc
    59641 agcgtgaagc ccagggtgat cacgttggac acgacagttc ccagcgtgcc ggtgagcgcc
    59701 cgctgggcgc cgatcacgtc gttgttgagg cggctgacga tcgcgccggt ctgggagcgg
    59761 gtgaagaact gcaacggcat gcgctgcacg tggtcgaaca gggcccgacg caggtcgtag
    59821 atgaggccct cgccgatgcg ggccgaccag aagcgctcga gcagcgacgc cgccgcggtg
    59881 agcgccgccg cgacgaccac caccgctccc aggccgacga gctgggcccg gttcccgtcg
    59941 gggatggccg tgtcgatgat ggtgcggaac agcagcggcg gcaccagcgc caggcccgcc
    60001 ccggccgcga tcatgacgac gaacccgacg agctgggacc ggtaggggcg ggcgaagcgc
    60061 ccgactcggc gcaccagggc gcgatccagg gtccggcccg ccacctgggt ggggtcctgg
    60121 tgcatcattc ggaacatcgc ggccgacacg cgcaggaacc tacagcggcg catcaggagc
    60181 gcttcaccag cgtgccaagc tgtccc
//
LOCUS       DAOQOD010000003        57835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_142_length_57835_cov_12.601194, whole genome shotgun sequence.
ACCESSION   DAOQOD010000003 DAOQOD010000000
VERSION     DAOQOD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 57835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57835
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_57835_cov_12.601194"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="PLZ72_00795"
     tRNA            complement(2..74)
                     /locus_tag="PLZ72_00795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Thr,seq:cgt)
     gene            complement(223..1305)
                     /locus_tag="PLZ72_00800"
     CDS             complement(223..1305)
                     /locus_tag="PLZ72_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34862.1"
                     /translation="MSADGPADGTDLDGPDLWGEVVGQDVAVARLRASVTDPVHAYLL
                     VGPEGSGTREAARAFAADLLLDGADPATVASGRRQVGAEAHPALTVVERVGASIDRDQ
                     MDDVVRRAHMAPPVGDRQVIVLVDLHLAAQSPKLLKTLEEPPPSTVLVVLAEEIPPEM
                     VTIASRCVVVEFDSVAQGAIAERLVSEGVDVEQARIAAAGAGGSMTQARLLANDPNAA
                     LRRDRWYSVPDRLDGTGTTAMALVDEVLAATDEVAAPLTERHDEELAAFDAQVDQLGA
                     RMAGPRNAMIARHKREQRRVRTADLLSGLATLVSRYRDALEDGAPADRFLAAADAVQE
                     LTDSLRFNPNVSLQLQALFVALPPLR"
     gene            complement(1302..1937)
                     /gene="tmk"
                     /locus_tag="PLZ72_00805"
     CDS             complement(1302..1937)
                     /gene="tmk"
                     /locus_tag="PLZ72_00805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNA34863.1"
                     /translation="MTGRFIVFEGGEGSGKSTQAGRLASRLGAVLTREPGGTDVGVRL
                     REVLLAAAEYDVALTDRSEALLMAADRAQHVATVVRPALAAGRHVVSDRYIPSTIAYQ
                     GAGRGLDAGDLSRLNRWATDGLTPDLVVLLEVPEPVAAERTGGARDRIEAAGADFHRR
                     VAASFAEQAAAAPELWVRIDGDGDADLVEERVLRAVTDRWPELGPVPGAGQ"
     gene            complement(1934..3631)
                     /locus_tag="PLZ72_00810"
     CDS             complement(1934..3631)
                     /locus_tag="PLZ72_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34864.1"
                     /translation="MSDLSAPTPEPPPAGGAPAQGWIERVFITRRFFALFNVQVISAL
                     GDWVGFFAITSLAASISTQPEAAIALVTTARVAPGIFLAPFIGVIVDRFDRKRIMVVS
                     DVARAGVFLLLPLVQTVPGLIVASFILEMFTLAWSPAKEATVPAVVPADRLTTANSLG
                     LVAAYATMPIAGPIQYVLKVLNDHLAEISVLAFLGLNRELGDTQTLAFYFDAASFLVS
                     ALLISRFVYRGLPSTSAAAREARAVEAAAATGDGSPSTFAEAEEAERSSLRGAFDDIR
                     EGLQFIGANPIVRGVILGLATGLIGGAMLVPLGPTFARFVIGNANTFPLFITALGLGV
                     ATGVVSLTALQKRLPKEQAFPVLVFAGGVSMFFGVSMSTFWLAALGIFGLGACAGAVY
                     VLGFTLLQEHTDDELRGRIFATMLTLVRACVLLALVVGPTLATVFNGFARAATGDQEG
                     VPTASVFGFDLAIPGVRLTLWLAALILCGAGVLASRSMKIGLRSQLRDGLRQAIGPNG
                     EERARGNHPTAIPVAAPDPSLPSEPEAEPTEPPANPPVTGSGPAAEPEPTPDAQEPT"
     assembly_gap    3723..3822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3823..6548)
                     /gene="topA"
                     /locus_tag="PLZ72_00815"
     CDS             complement(<3823..6548)
                     /gene="topA"
                     /locus_tag="PLZ72_00815"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNA34865.1"
                     /translation="MGSPLVIVESPAKARTIAGYLGDDYQVESSIGHIRDLPRNAADV
                     PKEYKGQAWARLGVDTENHFKPLYIVSPEKRDHVRHLKAALKDADELYLATDEDREGE
                     AIAWHLLEVLSPRVPVRRMVFHEITPEAIRHAIDNPRELDRRLVDAQETRRILDRLVG
                     YEVSPVLWKKVGRGLSAGRVQSVATRIVVERERERMAFRAGSWWDLQATFVHVDADRD
                     PTPFPAGLVELGGRRLATGKDFGQDGRLTASAQAAEVVLLDESSATAVCDALADRPVE
                     VRSVERKPYTRRPSPPFMTSTLQQEAGRKLRMSSSMAMSTAQRLYENGYITYMRTDST
                     TLSDAALNAARRQIRDKYGDAYVPDAPRRYANKVKNAQEAHEAIRPAGDSFRTPDQVR
                     SELGNAEFRLYELIWQRTIASQMTDARGESVQVRLETTTAPVVVPTVDGADPSAAVEQ
                     VAVFSASGRTILFPGFLRAYVEGRDDPDAALDDQERILPDLAVGEAVRAEDLEVAGHE
                     TQPPARYTEASLVKRLEELGVGRPSTYASTISTVQDRGYVWRKGTALVPSFTAFAVVT
                     LLEQHFPTLVDYAFTAAMEEDLDRIASGSEDMEPWLSRFYFGGAGGAPAEGDPAGDAD
                     VAAGLALKRLVDDLGDIDARAINSIPLGADGDGVPIVARVGRYGPYLERGDGDDVQRA
                     SIPDDTPPDELTPERAVELLEAPSGDRDLGEHPDTGLVVHLKAGRFGPYVQEGEHDDE
                     TGLKPRTASLFASMDPATVTLEQVLPLLELPREVGADPADGVVITTQNGRYGPYLKKG
                     TDSRSLDSEDQILTLTLDEALAILAQPKRRRGQAAPKPPLKELGPDPDTERPIVVKDG
                     RFGPYVTDGETNASLRKGDSVEELTMDRALELLAERRAKGPSKKKKATKKKST"
     gene            6758..7969
                     /locus_tag="PLZ72_00820"
     CDS             6758..7969
                     /locus_tag="PLZ72_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA34866.1"
                     /translation="MNLEPSDRGKALQETLLAFMDSHVYPAEPVYREQMAASGDAHFH
                     PPIVEDLKAAARERGLWNLFLPHRTPWSPDPLPNLDYAYLAEITGRSTALAPEALNCA
                     APDTGNMEVLALFGTPAQQEEWLVPLLEGEIRSAFAMTEPDVASSDATNIQMRIERDG
                     DDYVLNGRKWWTSGAASDRCKIMIVMGKTDPDAPRHLQQSMILVPMDTPGVTKVRDLP
                     VFGYQDREGHGEMLFEDVRVPATNLLAEEGSGFAIAQARLGPGRIHHCMRCIGIAERA
                     LDLMCRRVTERVAFGRPLSEQGVIREWIADSRIEIEQARLLTLKAAHLMDTVGHKGAA
                     IEISAIKVVAPNMALRVVDRAIQAHGGGGVSDDFPLAQMYAGLRTLRFADGPDEVHRR
                     QVARQELARHT"
     gene            complement(8004..8327)
                     /locus_tag="PLZ72_00825"
     CDS             complement(8004..8327)
                     /locus_tag="PLZ72_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNA34867.1"
                     /translation="MLFDVRIVPAGRWDLLCVVGDMDLATIPALRQALDRTESPDVAV
                     DLSGVDHLDPVTLGVLFVGSLRARRAGGRFAVVCPAGPARDLLVELGVDRLLEVLAAQ
                     EDLPT"
     gene            8397..>9058
                     /locus_tag="PLZ72_00830"
     CDS             8397..>9058
                     /locus_tag="PLZ72_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA34868.1"
                     /translation="MTTRSDPSETPQGPAVGDTDDCVRDTVDALVAEWSAEPRLVHVE
                     RLSGRPARLARPAEPLHPALEDRLGERPLWSHQARAIDLVRSGRSVVLSTPTASGKSL
                     CYQVPAVDAVLERGASTLMVFPTKALAQDQLRTLTGWDLPGVVAATYDGDCTPEERTW
                     VRGHADILLTNPEMLHHGILPNHGRWATFLHRLELVVIDELHVLRGVFGTHTAQVLRR
                     LRR"
     assembly_gap    9059..9158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9159..10761
                     /locus_tag="PLZ72_00835"
     CDS             <9159..10761
                     /locus_tag="PLZ72_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNA34869.1"
                     /translation="EPAATGPIDELEQIEDAADGVDRRTSVHAEAATVAARLVTAGLR
                     TLVFCRSRKATELVAAEIRHRLDPEHADGVRAYRAGYLVGERRDIEDDLFSGRLHCVV
                     ATSALELGVDVGGLDAVVLAGYPGTVASFWQQVGRSGRSRRPSLAVLVAGEDQLDQWM
                     VRHPDDLFSRAPEPAVINADNPFVYVPHLACAAQEQALRRDDERMWPDQLDEGVRRLV
                     LGDRAAVRRRRGQREVVWTGRGLPAPTIGLRSSSRGEVRIVDGDGELIGTVGADRAPA
                     TVHTGAVYLHQGRPYSVTDLDLDARTAVVEPTSGDAYTRTRSETSIRVLERDRHRVVG
                     AAGLALGSVEVTSQVVGFQTRSVATHEVLDTTPLDLPPSRLVTRAVWYTFDDDLVGRS
                     EVGDRELPGALHALEHAAIGMLPLFTICDRWDVGGVSTAWSPDTASATVFIHDAHEGG
                     AGIAELAFDAADRHLAATLELIDACRCATGCPSCVQSPKCGNGNEPLSRDGAVRLLRA
                     TLCSTDTGAGSGPSVDSAIDDRHAS"
     gene            complement(10780..11169)
                     /locus_tag="PLZ72_00840"
     CDS             complement(10780..11169)
                     /locus_tag="PLZ72_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNA34870.1"
                     /translation="MSVRRPDRWASPDARGQATVEIAMLLPVLVTTLLLVVQVGLLAR
                     DRVLTVHAARTAARAAAVDPDPASVRGSLRDVGQGRFRVEIGGDTAPGGVARVRVSTR
                     PTSVPVVGRLVGGIELSEALAVRVEGA"
     gene            complement(11166..11462)
                     /locus_tag="PLZ72_00845"
     CDS             complement(11166..11462)
                     /locus_tag="PLZ72_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HNA34871.1"
                     /translation="MTVPMSHPYPDHSDTGRAGPTRSRASRPRSGGPGAALWARWYTR
                     QRVLDEQAQSTVEYALVLLGAAAVALALVAWVTRSDAVSRLFDAIVGRILSQAG"
     gene            complement(11459..12250)
                     /locus_tag="PLZ72_00850"
     CDS             complement(11459..12250)
                     /locus_tag="PLZ72_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA34872.1"
                     /translation="MGPLMPWLLAAAAAVAVADLVVPRPRPRRFPEDEPGSAVRAEPT
                     RASATAVVDRAFRAILVARLQTARPRAERSPERPLARPTAGGRSVEELWDMAHCCDLL
                     AVAAAAGSTVAGSVEAVGDVGDGPVASALGRAGADLQRGKVLADVIASLRHELGPAGQ
                     PLVTTLSTAASSGTPPAAALVRLADAERRRSRRRIEARVRRLPVLLLIPLVALVLPAF
                     VMLTVVPVALSAARGTGALLGAATASAGPFPLPPSAPFVPRGGPP"
     gene            complement(12238..12915)
                     /locus_tag="PLZ72_00855"
     CDS             complement(12238..12915)
                     /locus_tag="PLZ72_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34873.1"
                     /translation="MTLLVLAVVGITAGGVALAGSGRRRRRRLATRAARDLPGAVGDL
                     ARSVGSGATLTVALRELAGGPVGLLGTEVAGAVALLDRGLGVDRVMELWGRASRIDGV
                     ELLVAACRFSRGRGVALVAALDGVAAALVDRIELDDEVRALTSQARTSATVLVALPLI
                     GVTVLAAVDPSFAGVVLGSGAGRACLVVGVVLDGLGAVVSNRLVTRAVAAPGVQGSGR
                     RNRRWAR"
     gene            complement(12912..13985)
                     /locus_tag="PLZ72_00860"
     CDS             complement(12912..13985)
                     /locus_tag="PLZ72_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNA34874.1"
                     /translation="MARTDPELVAVVGRRFRSEPDGDRGDDAVGRLARIVREERPLMG
                     DAAVVETARALAVDLVGLGAVERLLGDPTVTDVLVNGPGEVWVERAGQLQTTGIVLDR
                     AAVDRAIERLVGPLGLHADRAHPVVDARLADGTRVTAVLPPLAVDGPVLAVRRHRRDP
                     IPLDSLARPDVAALLRERLVGRRNLVVFGATGSGKTTLLASLASELPDHERIITVEDV
                     AELRLPGRGVVRLEARPGGAAGVGRADFRDLVRVALRLRPDRIVVGEVRGAEAADMVW
                     ALGTGHDGSMSTVHAPTASDAVDRLTSFAVAGADGAPVDVVRQQVHAALHTLVGVERR
                     PDGRREVASLHDLGSDGRLVPVT"
     gene            complement(13991..14656)
                     /locus_tag="PLZ72_00865"
     CDS             complement(13991..14656)
                     /locus_tag="PLZ72_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HNA34875.1"
                     /translation="MLFTLWSVKGGSGTSVVSAGLATLLTQRGREVLLVDLAGDQPAL
                     LGIPEPTGPGVGDWLGSPDGDAAALGRLEIEVADGLRLLPGDLNGAIGAERVSDLVEV
                     LRSDRRTVVVDAGVRSPGSALRSALCEEGISVLVVRACYLALRRAVRSQVTADSAVLV
                     VEPGRALDGTDVSRALGIPVHELDWDPTVARAVDAGLLSSRPPRGLARDLAPLAEIGE
                     SRP"
     gene            complement(14853..15785)
                     /locus_tag="PLZ72_00870"
     CDS             complement(14853..15785)
                     /locus_tag="PLZ72_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA34876.1"
                     /translation="MTDQRRERDDEDLVRRYLAEIGRHDLLTKDDEARLARVMEDGAA
                     AARELGSGAAIPATRRRQMQRRVRAGEAAAEQFVQANLRLVVSIAKRYQSSGVPLLDL
                     IQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRIPVHAGDTMTR
                     VQRTRSRLEIELGRMPTTAELAREVDLPEDKVTETLLYAVEPLSLDEPLREDADAVLA
                     DLVADDAPTAEGAALHAMVPTAVDDLLSGLEEREREVLRLRYGLDRGEPRTLEEVGAQ
                     FELTRERIRQIEVRAMSKLRHPSNESGARDLLLV"
     gene            complement(15954..16526)
                     /locus_tag="PLZ72_00875"
     CDS             complement(15954..16526)
                     /locus_tag="PLZ72_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34877.1"
                     /translation="MTPDVVDGYPPASTVAGEFRDDSGPLDLLSSAGRLGSWAWTEAR
                     LHRIVGGWAPDADGAHVVVVLDRLAMGHAARSALLVDRLPRLRELPRDTLVRSPGPAA
                     DAVLELLGALSQGDRLAAWVVVASALADAYDTHLLRCTPVADRPLLARLPAQVDRLRA
                     DAADVRTRVGPDRPPVEMLDLLEATCGFTA"
     gene            complement(16531..>17521)
                     /gene="nadE"
                     /locus_tag="PLZ72_00880"
     CDS             complement(16531..>17521)
                     /gene="nadE"
                     /locus_tag="PLZ72_00880"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNA34878.1"
                     /translation="VDVSADVAVDGPSGVGPVLAVSEPAPGRDSLTPVDALVEPSPAG
                     PEEIWDALVLGTRDYVTKNGFTDVTLGLSGGVDSALVSAIAADAVGAERVHAVLMPSR
                     YSSDHSLADAHHLASNLGVDARTIPIEAAHAAFLELLAPSFGERPPDLTEENLQSRVR
                     GVLLMALSNKFGWLVLTTGNKSETAVGYSTLYGDSAGGLAVIKDIPKLLVYELCRWRN
                     EREGAALIPESILTKAPSAELRPDQRDDQSLPPYEVLDPLIEAYVDGDQTLEDLVAAG
                     HDDAMVRRITRLVDLAEYKRRQSPPGIRISQKAFGRDRRLPITNGTTRDSGTA"
     assembly_gap    17522..17621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17622..18382)
                     /locus_tag="PLZ72_00885"
     CDS             complement(<17622..18382)
                     /locus_tag="PLZ72_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNA34879.1"
                     /translation="MTPEPSTAPGPAVVLRVALCQLDLTVGALRSNVAAMADAYRIAE
                     AAGCDVALFPELATCGYPPEDLLLRPGFVADNAAALDSLAAQVTGHCAAVVGWVEGHR
                     TPGADPHDSTDGPWNAAAVLHDGRVLGSYRKQALPNYGVFDEKRYFDPGSAGQPLFRI
                     AGVLAAVTVCEDLWVPAGPALDATRRGAHVVLNINASPFHAGKQELRESTLLDRQAEC
                     SVPIVAVNLVGGQDELVFDGGSFVVDRGEVQARARR"
     gene            complement(18464..19480)
                     /gene="glnII"
                     /locus_tag="PLZ72_00890"
     CDS             complement(18464..19480)
                     /gene="glnII"
                     /locus_tag="PLZ72_00890"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HNA34880.1"
                     /translation="MAYRAEYIWIDGTEPTPLMRSKTKIVPDGDEPGLWNFDGSSTNQ
                     AEGSNSDCMLNPVYTVPDPTRGGNDVLVLCEVLLPESLEPHSTNNRAACVEIADKFAD
                     FEPLFGLEQEYTFFKDGRPLGWPQEGYYYPAPQGPYYCGVGAVEIAGRDIVEAHTSAC
                     MAAGLSISGTNAEVMLGQWEFQIGPCGTVEVADQIWMARYLLYRISEDFGVNASISAK
                     PIKGDWNGAGCHTNFSTRQMRESYDAIIAACEALGAEGKVEEHLAGYGHGYEDRLTGQ
                     HETAHFSQFRYGVGDRGASIRIPWQVARDQKGYIEDRRPNANIDPYVVTRLMTNTVCG
                     AASA"
     gene            19744..21075
                     /gene="glnA"
                     /locus_tag="PLZ72_00895"
     CDS             19744..21075
                     /gene="glnA"
                     /locus_tag="PLZ72_00895"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNA34881.1"
                     /translation="MDHQQDYVLRTVEERRARLVRLWFTDVLGQLKSVAISPVELEAA
                     FEEGVQFDGSAIDGFSRVQESDVLAFPDPNSFQLMPARDGEEVTARIFCDITRLDGAP
                     FEGDPRQVLKRNLAAASEAGFQFMCAPEVEFFYLASDDPRDPQPLDSASYFDLTTYDE
                     TSDIRRRTIQRLEGSGIPVEYSFHEDAPSQHEIDLRYADALTMADDVMALRMIVREVA
                     MASGVHATFMPKPFEGVQGSGMHTHLSLWSGDDNAFHDPDDAVGLSATARSFIAGLLV
                     HAGELTAVTNQLVNSYKRLVSGFEAPVHISWARNNRSALVRVPVPKKGKEDQGTRLEY
                     RALDPACNPYLAFSVLLAAGLAGIRHGYELPEETTVNLFESGVDAPADLGRRLPANLA
                     EALDAMERSELVREALGEHIFEWFLRNKRAEWGAYRTHVSSFELNRYLRTW"
     gene            21082..21753
                     /locus_tag="PLZ72_00900"
     CDS             21082..21753
                     /locus_tag="PLZ72_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA34882.1"
                     /translation="MQPLLVWPDPAPPDVARVLDLAGLPWRAVADEAVARDGVWSGAV
                     VAADEDPERGFAMARTLRRGDLAVAPLLLVVRGTQLADLDGRHDTFDDFCLAPAHPTE
                     LEARVRHLLAAEPGLALEPDVVQYEALVLNLETYQAAIDGTPLDLTYMEYELLKFLAS
                     NPGRVFTREILLSRVWGYEYYGGARTVDVHIRRLRAKLGEEHAGLIQTVRSVGYKFGE
                     SRWGS"
     gene            complement(21814..21972)
                     /locus_tag="PLZ72_00905"
     CDS             complement(21814..21972)
                     /locus_tag="PLZ72_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34883.1"
                     /translation="MIGPLLIIVVLVVALPIAFIITGGVISVILGQSLAKEGEYLNEG
                     SELIDLNV"
     gene            complement(21978..22319)
                     /locus_tag="PLZ72_00910"
     CDS             complement(21978..22319)
                     /locus_tag="PLZ72_00910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNA34884.1"
                     /translation="MTAPYVPARRAGDWLVLSGQIGLGPDGLADDFAGQLRQALANLD
                     ELLAANGARSDQVTKTTVFLADMDDYAEMNEIYTGYFTEPRPARTCVAVAGLPLGARV
                     EVEAWVFTGAD"
     gene            complement(22448..24103)
                     /locus_tag="PLZ72_00915"
     CDS             complement(22448..24103)
                     /locus_tag="PLZ72_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNA34885.1"
                     /translation="MNASPSPDEPSAEVPTRAVGLTVDGRDVEVVDDGISLLDALRGP
                     LGVHSVKDGCSPQGQCGCCTVLVDGTPRVACVTPLRRVAGRSVVTLDGLEPEARSRWV
                     DAFVEHGASQCGFCTPGIIVRLDAVRAKGGDVRPGAVDRALAAHLCRCTGWQTIVEAA
                     EDVLSGSTPVEVGPRDLDAAARRATLELGAPQQVGERVVLGAVGFSADTAPADALIAV
                     PVPGSDDWVVGESLVEARRAAGKVQGRRTTVDPVPPLEVPDGDWDLTLRTGWVDAAYV
                     ETDSSWCVPGGDPSPAAANGGAFGAKRHSPLPAVARRLADEHGRAVLAQWSREDCSRS
                     APKRPPVAAGIRADGTGVIHVVRTEGIAERIRAAAPGLEVVELDVAGPPTSVTLRGAG
                     WAEAVVLVAGVGAPSEVVSVESGRVGDQAGGERVVVRTSEGAVAEASIGADGIRVRAA
                     GGDILDPVVLRSYCIGAAHMAFGWVTSEGVAVDAEGSVLDLTVRSLGVLKAADTPAIT
                     VELVPSDRPPVRVSDAVFAAVAAATWRFLGTPPDWPAGLLSNL"
     gene            24205..25149
                     /locus_tag="PLZ72_00920"
     CDS             24205..25149
                     /locus_tag="PLZ72_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34886.1"
                     /translation="MASHPHPRRPGAGSDAPTAGPSRRSVLRGGVGLGAATVGGILLG
                     SCSSEADTRSADNLSSHALLAAFPQTLPHIPAGVPFRLPYLISDDEGVPLTTIDGAVT
                     FTVSRDGHELGTVRVEPHSDGVPRAYLPVGFTFPEPGRYDLTATYDGAPMSSTVEVVE
                     PARVGPPVVGQKLPPVDSPTTERTLEVDPLCSRLKGPCPLHSVNLRDAVGLGKPIVVV
                     IATPAYCSSDVCAPLLELVLEQTAGRDDLVVTHQEVYKNPKGQRDLSDAQLAPVPDAY
                     RLRFEPVLYVTDRTGTIVARADITVDRGELSELLALAV"
     gene            complement(25202..25519)
                     /gene="erpA"
                     /locus_tag="PLZ72_00925"
     CDS             complement(25202..25519)
                     /gene="erpA"
                     /locus_tag="PLZ72_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNA34887.1"
                     /translation="MITLTDNAATKVKQLIDAEGEEGLALRVEVRPGGCSGYSYEMYF
                     DTDVAADDIRNTYGDVDVVVDPTSAQLLQGATLDYKDGLEAGFAITNPSAQSSCGCGQ
                     SFS"
     gene            complement(25610..26296)
                     /locus_tag="PLZ72_00930"
     CDS             complement(25610..26296)
                     /locus_tag="PLZ72_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA34888.1"
                     /translation="MATSERRLDLLKTLGDNTRYAIYLELARSPRPLATAEIADTLGL
                     HPNTVRPHLERMREVGLVDARPDSRGAVGRPQHLYSLTSDAPSLGLEPPMFLMLARML
                     LELAVDAGLGGEPVLAAGREQGRRLAHHEVLGDALDPTRRPCVDASLSMLAELGFDPA
                     AVTEADATTVAFGHCPFGELAESNPELVCALHRGLMEGFAGEIGGASVVDFHDLSHRE
                     PCRATLVPDR"
     gene            26355..27629
                     /locus_tag="PLZ72_00935"
     CDS             26355..27629
                     /locus_tag="PLZ72_00935"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNA34889.1"
                     /translation="MTDQTPPASTDVLSAPTAGRVSEEAVWEVLRGVIDPELGSDIVE
                     LGMAKGVRIEPDGTVHVTIALTTSGCPLRAEIQRNVRTRVGALAGVTHVKLDWTELDQ
                     AGKAAAMERARRNIAERAEQTAVPSSTKVVMVASGKGGVGKSSVTVNLGAALAAMGFT
                     VGIMDADIWGYSVPRMVGLDGRMHAEEQPPEPDGAAAGDKRARIIPNEIPVGDGRLKV
                     VSMGFLVEDEGSALMWRGLVLNRAVQHFLEDVDWEGVDYLLIDMPPGTGDVQMGLAKL
                     LPRAEMLIVTTPSLTAQKVAIRVVDMGRKNFLHIAGVVENMSAYVDDTGREHRLFGTG
                     GGDRLAEDAGVPLLARIPIEAAVAHGGDHGRPVALADGPAADAFRALARRIVEDVIQP
                     TDMSGCSSRMLDAVQAALDEADSGPVATRAPS"
     gene            27666..28559
                     /locus_tag="PLZ72_00940"
     CDS             27666..28559
                     /locus_tag="PLZ72_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA34890.1"
                     /translation="MKIRVGFGLGVRTRFVDRSFLDLVDDLERLRFDSLWLSERVTAE
                     CPDPVVALAVAAGRTTRLKLGMSVMVLPGRNPVLVAKELATLDRLSGGRLLPAFGLGA
                     VSRAEQQAFGVERGERAARFDEALGLVRRLWTEDRVDHEGRFFRLEGAKVRPFPHQSP
                     PDVWLGGAAPAELRRVGRLGDGWLPSFITPEAAAAGRAVVEDTAAEHGRSIDPEHFGV
                     LLPYSFDPVPDEVLELLPARDPDIDRRRLVAVGAEQLADRIGEFVDVGFSKFVAVPLG
                     DPVDATDHLERLSRDVLPLQT"
     gene            complement(28600..>28893)
                     /locus_tag="PLZ72_00945"
     CDS             complement(28600..>28893)
                     /locus_tag="PLZ72_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34891.1"
                     /translation="RELIGTQWLTPAEALRRHDAGDITMIFPTVRTLVALSRFDRADA
                     VLDHARAQSRVEGILPTISDGDDGMRIVLPGDPEHVGGVYDAWTARPLSPDHD"
     assembly_gap    28894..28993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28994..29331)
                     /locus_tag="PLZ72_00950"
     CDS             complement(<28994..29331)
                     /locus_tag="PLZ72_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA34892.1"
                     /translation="MSPVPMDSPTPEQVPVRDAATVMLLRDGSDGPEVCLMQRNLNSD
                     FVGGAYVFPGGAVDPADADADVADRCPDLDDAEASRRLGLAVGGLAFWVAAIRESFEE
                     AGVLLARHADG"
     gene            complement(29328..29882)
                     /locus_tag="PLZ72_00955"
     CDS             complement(29328..29882)
                     /locus_tag="PLZ72_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNA34893.1"
                     /translation="MVLLVLLLLPILEIGAAILVAQAIGLLPTLLLLVGLSVLGVVLI
                     RAEGPQTWRRANAELAAGRAPTDTLLDGLMVLVGGVLLIVPGFLTAVPGLLLLFPPTR
                     ALLRPVMTRWVERRAARSAVILTSFGGPGGPGGAGFGSFRIDAPFGRGPVVDADSHER
                     RPSTAYAEVVEVDVDAPREIGPPT"
     gene            complement(29986..30246)
                     /locus_tag="PLZ72_00960"
     CDS             complement(29986..30246)
                     /locus_tag="PLZ72_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA34894.1"
                     /translation="MSAQQVHDMWQVKAACRGPQAAVFFPPPQFERKADRLERERRAK
                     AICADCPACQECLEYAVAIREPHGIWGGLNETERRVLIEAAG"
     gene            complement(30420..31760)
                     /locus_tag="PLZ72_00965"
     CDS             complement(30420..31760)
                     /locus_tag="PLZ72_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNA34895.1"
                     /translation="MIVRPDAPTRPGPLRRPAAARFAARFALVLVGLGTLAGWAVSPA
                     GAQTNSCAKHGCVDVVAVNGLVDGIEADFIVDTLASARRSDGVVGVVLQFDSGGSAVG
                     DERLDQVARAIVDSDVPVSVWIGPSGSEALGGAAELVQVADSSGIAPGASIGDVGSQR
                     LSVDEFGDLFTGKKAKGLEATLTGQAAVRAGLVTRFSPTVGDHLVNLDGVKTETRTED
                     GERRTVPLTTVRFSKLPLTTQLFHTVASPSVAYLLLTIGLGLLLFEFFTAGIGIAGVV
                     GALFTVLGGYGLAELPHAQWALVVFVASFVAFGIDVQTGIPRLWTAIGLVGFTVSSVF
                     LMTDFRPTWIALIAGIGGIAATMITGMPAMVRTRFSTPTIDRDWLVGESGRAATPIDP
                     EGNVKVAGGLWRARADTDASVPSGAAVRVVAVDGVVLEVEADDTGLTDAPDPSD"
     gene            31812..32828
                     /locus_tag="PLZ72_00970"
     CDS             31812..32828
                     /locus_tag="PLZ72_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNA34896.1"
                     /translation="MDPAQFLTASGVIIVAGKGGVGKTTVTAALAVAASAVGLRTLIV
                     EVEGKGGMSSMFSADGFAYDEVQLIAAAADHAGVRARTITPDDALLDYLGDRGLQRIS
                     NRLVATGLLDIVATAVPGIRDILVLGKVKQLERSRDQDLILLDAPAAGHAITFLRSAS
                     GLADAVKVGPINTQAEDVLELLGDPGRCRVVLVTIPEETPVNELVETAYSLEDTVGVA
                     LGPVVVNGVVPDLAGLDVPPVEAAAAVGTRLLDGEAEVLSEAAAFRRARGRLQDDQLR
                     RMADELPLPQLHLPFLFTTDVGPAELETLADALLEAIRDLPDDPAHDPDPAAVAGSPS
                     SGTP"
     gene            32825..34030
                     /locus_tag="PLZ72_00975"
     CDS             32825..34030
                     /locus_tag="PLZ72_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNA34897.1"
                     /translation="MTARTLSEVVDRASVVVCCGSGGVGKTTTAALIAMHAAQQGRRS
                     VVVTIDPARRLADALGLQGLTNDPSPITGPWNEPGGAGGSMSALMLDTKGTFDGLVKR
                     YSADRGQAERILANAFYRNISGALSGTQEYMASEKLYELAVEGDFDLVVVDTPPTRNA
                     LDFLDAPQRLAHFLDHRLYKVLLSPSKGIGRAVNVATQAVMRQIAKVVGAEVISDALA
                     FFKAFEGMEQGFKERAREVDRLLKRPSTAFVLVASPRSDTVTEAGFFADELARKEIAV
                     QGLIVNRTQPSFGVALGDGAGEGTDGPVRVALSGSAVRARAETLAGTALGDHYRCLAD
                     SLHLAAGEAEHLAGLAERVTPAPVVKVPVQPFEVSDLESLRTLAAVVFADTSDGYGAG
                     DSDAQQAHD"
     gene            34041..34436
                     /locus_tag="PLZ72_00980"
     CDS             34041..34436
                     /locus_tag="PLZ72_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34898.1"
                     /translation="MTTYLIATDSDDLFGLLDAAIGGTASLVRVSSGQAVRPAVRELE
                     PDLVVLDLQIGTMGAMAVCKDLRLEEGAGRLPRQNVLMLLDRPADVFLADRSGADGWV
                     TKPIDPLQVRRAARAVVAGDGYRHQPSPA"
     gene            34511..34587
                     /locus_tag="PLZ72_00985"
     tRNA            34511..34587
                     /locus_tag="PLZ72_00985"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34545..34547,aa:Pro,seq:cgg)
     gene            34700..35206
                     /locus_tag="PLZ72_00990"
     CDS             34700..35206
                     /locus_tag="PLZ72_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNA34899.1"
                     /translation="MAAELDTTEPIALDRGRVPRRRRGVVAVVAVVLAVLMSGALTAC
                     ESNKAELDAVRNAVNASRAQAGLPALRENVTLDVKADGWARTLRDSCRIWHSRLSDGA
                     PKEWRKLGENVGMGGSIDQIHVAYMNSPGHRANILDPAFNQIGTAAVWGTCNGMRTLF
                     TVHVFMKG"
     gene            35246..35500
                     /locus_tag="PLZ72_00995"
     CDS             35246..35500
                     /locus_tag="PLZ72_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNA34900.1"
                     /translation="MEIVAFQRLMEDLYGDCDRQRGVPSTVAWLCEEVGELAQAVRKG
                     SVEDQLHELGDVLAWVASLANQLGLSLEDAANRYVTDPPV"
     gene            35574..35957
                     /locus_tag="PLZ72_01000"
     CDS             35574..35957
                     /locus_tag="PLZ72_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNA34901.1"
                     /translation="MDLHEVVDDVVSVIEAVGVVVLVVGASLAFITSGRELLRRSGSP
                     GTGAYDDLRRNLARVILLGLEVLILADIIKTIVVDQTPQSVAVLGIIVVVRIVLSWSL
                     QVEIEGTWPWARWRTGTAAAPDRMD"
     gene            complement(36023..37102)
                     /locus_tag="PLZ72_01005"
     CDS             complement(36023..37102)
                     /locus_tag="PLZ72_01005"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNA34902.1"
                     /translation="MSTHPDAIAGPDLSGARPRHIGVFGATGQVGGVMRTLLAQRGLP
                     VASIRYFASARSAGTLLPWKDTQVEVEDTATADFAGLDIALFSAGATSSREYADRVAA
                     AGATVIDNSSAWRIDPTVPLVVAEVNPHALDDIPRGIVANPNCTTMAAMPVLKPLAVE
                     AGLRRLVVSTYQAVSGGGLAGVEELDRQAQAVGTKGPELTYDGGAVAFPAPEKFARTI
                     AFNVLPLAGTVVDDGSEETDEEQKLRNESRKILDLPDLAVSGTCVRVPVFTGHSLSIN
                     AEFERDLSPERAREVLATAPGVALADVPTPLDAAGRDPSYVGRIRRDPGVEHGLALFV
                     SNDNLRKGAALNAIQIAEELIARQG"
     gene            37169..38314
                     /gene="hemW"
                     /locus_tag="PLZ72_01010"
     CDS             37169..38314
                     /gene="hemW"
                     /locus_tag="PLZ72_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNA34903.1"
                     /translation="MAFGLYIHVPFCARRCGYCSFVTYAPGELTASQTHRRWAEAAVA
                     EVGVAAAGLGGHDDSEPTVVDTIYFGGGTPTAVDPALLAGVLDAVRERFAVAGDVEIS
                     VEANPDGLAPGTLESLAGLGVRRVSFGMQSVVPRVLALLDRTHDPESVPAAVAAARAA
                     GIPSVSLDLIAGTPGETDVDWANTVAAALAMHPDHLSAYSLGIEGGTRLAARVRRGDL
                     VEPSPDEAASRYEHLDAAASSAGLSWYEVSNWARTTADRCRHNLGYWRDDDWWGIGPG
                     AHSHIDGRRWWNHSGLDDWSTEALAGRVPAAGSETPTAEERLTEHIMLGIRLPEGIST
                     DRVTDGSVDSLVDDGLVRRLPGPTQRIALTLRGRLLADLVVRRLRWG"
     gene            complement(38416..39639)
                     /locus_tag="PLZ72_01015"
     CDS             complement(38416..39639)
                     /locus_tag="PLZ72_01015"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNA34904.1"
                     /translation="MALLVQKFGGTSVGDPERIREVADHVARCRRRGDDVVLVVSAMG
                     KETDELLRLAGEVSSRPPGREMDMLITAGERKSIALVAMALAERGVESESYTGSQAGF
                     VTDTNHTNAKITDVRADRIRAAVAAGRVPVVAGAQGVSTDNDVTFLGRGGSDTTAVAL
                     AYALGADACELYTDVSGVFTTDPRVVSTARRMHRITFDEMLEMTAAGCPKPAMRSVEY
                     AHRHKVPLHVRSAFTWEPGTWVIEEDPDMEDALIRAVQADDSEAKITVTGVPDRPGVA
                     ATLFRSLADADVNVDMIVQNVSEGGVTDISFTVPHAQIDEAIAVCEERAAEVGAAAVV
                     TDRAIAKVSVIGAGMKSNPGVAATMFQVLADNGINIEMISTSAIRISCVIAEADSDRA
                     VQVLHDAYELESTTF"
     gene            39793..>40502
                     /locus_tag="PLZ72_01020"
     CDS             39793..>40502
                     /locus_tag="PLZ72_01020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA34905.1"
                     /translation="MGTSAEKHARQKQARSSKLQEATVRARQSSRRRRLVTVIGVLLG
                     VGIIVAGVWILTGSGDDDTAEGSTTTTTAGDSTTTTAADAAELPRPPEGITLTEKTPC
                     PPAAGTEKRVERFAGPPPTCIDPDASYTATFSTTKGDFTATLDSKKAPVSVNNFVVLS
                     RYHFYDGVPFHRIVPGFVIQAGDGDGDPWGNNDLGYSIHDELPASSAEYVDYSLAMAN
                     SGPNTNGSQFFVVLPGGGA"
     assembly_gap    40503..40602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(40603..41283)
                     /locus_tag="PLZ72_01025"
     CDS             complement(40603..41283)
                     /locus_tag="PLZ72_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNA34906.1"
                     /translation="MTGTLALVGGREWTEGCTFDEALVAGVTEVLVLPSALAYENPAA
                     AVDRARAWFGRWGVAVSVLDVYRRAEALEPGPAERAADAQMVYVVGGSPMHLRSVLKD
                     TPLFDALVGAWRAGATVAASGEAASVLSSHMVDSRGGAYTVGLDLIETMTVIPRSNGW
                     SPEKLHRTVELARPGLAVVAIDEATALIHSPDGTWRVEGAGNVTVYRDGHPADLADLP
                     SLLRPEPA"
     gene            41354..42211
                     /locus_tag="PLZ72_01030"
     CDS             41354..42211
                     /locus_tag="PLZ72_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNA34907.1"
                     /translation="MTIERVGILGSGIMGSGIAEVAAKSGFEVVLRSRKQESADAMVA
                     ALEKSLARQVERGKLEQATADEVRARVTATDHLGALVDCDLVIESVVEDLAVKKPLFA
                     ELDSVVKDSAILATNTSTLPVVDMAVATERPDRVCGVHFFNPAPAMALVEIVRPLTAS
                     DETIAAVTEFASACGKNPVQVKDQAGFIVNALLFPYLNNAVRLLENGTATAEDIDTAM
                     KGGCNFPMGPLALLDLVGLDTSLSILDALYDEFRDPNYAAVPVLRRMVSAGQLGRKSG
                     AGFYDYKKG"
     gene            42260..42910
                     /gene="aat"
                     /locus_tag="PLZ72_01035"
     CDS             42260..42910
                     /gene="aat"
                     /locus_tag="PLZ72_01035"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNA34908.1"
                     /translation="MFPPLHDADPDGPVVLGGDLEPGTLLAAYRSGLFPMPIGRRRLG
                     WWSPDPRGVIPVGPRAVAAGTALHVSRSLRRSLRRFTATVDADFEAVMRACGDPRRPH
                     GWIDENFVGAYTELHRRGWAHSVEVWEDDQLVGGVYGVSIGAFFAGESMFHRATDASK
                     AALVHLTDRFAHVEDALFDVQWLTPHLASLGAVEIPRSEYLTRLASAVARPVDPFG"
     gene            complement(42923..44056)
                     /locus_tag="PLZ72_01040"
     CDS             complement(42923..44056)
                     /locus_tag="PLZ72_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HNA34909.1"
                     /translation="MTDPPSPVGTEPAGGPAADQSASAGPTRPSRRKQVIAGLVTLVV
                     LVIVFFGIFPKFANYGEAWESIQEMSASSLALFALATVANILIYVFPYQAALPGLAYG
                     RAFIVRQTSFMISNAVPAGGAFGLGVQYAMLNGYGIDPAVSTSAIGVTSVWNLMVTLA
                     LPALGLLALVLSDEATSGALLGAVLSLVALGVMVGGLALILRSEGWARRLGGWGDALL
                     ARLRPRTAPAAGEDGPLTAGALRFRDQTVDVVHDRWIRLTVTNFGQQLSQFAVLAVAV
                     YGLGGKSTGINLLELFAAFSLARLAGFIPITPGGLGTVDAALVGLMSAFGMDKDQALA
                     ANLLWRAATFIPQVVIGIVTFLIWRVEQNKRLGGSTVAAAPSA"
     gene            complement(44144..45481)
                     /locus_tag="PLZ72_01045"
     CDS             complement(44144..45481)
                     /locus_tag="PLZ72_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA34910.1"
                     /translation="MTDTSTDQSDAGSSDEILEELQAWLDQNWDPELTVGEWWERLGL
                     SGWAAPGLPTHAYGKGLSRNDSVRAQAAIAEHGALGAPGGLGLLLAAPTIATHGTQAQ
                     IDHYVRRIVTGVDAWCQLFSEPGAGSDLAGLTTRAVEDGDEYVVNGQKVWTSLGHTAD
                     MGMLIARTDPDVPKHQGITWFAFDMHQPGVEVRGLKEMTGHAMFNEVFLTDAHVRADA
                     IIGDRNNGWAVTNTTLMHERAGLGSGGGSGAATSVSPGSVADQLGARAGDFAPKKSGA
                     RKGGGGAIGMLGSMPKMLQDLARSNGALDDPTVRQDLVRLHTLGELGRYNNLRLKAAK
                     QAGGDIPGMPNISKLAMSDMMRLIRDLGLRIVGPAGTLHAYEDADKAAIEDATGNPFL
                     NLVTWVSLWAQGPSIYGGTDQIQRNIIGERVLGLPKEPNNDKTLPFSELPKNA"
     gene            45643..46755
                     /locus_tag="PLZ72_01050"
     CDS             45643..46755
                     /locus_tag="PLZ72_01050"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HNA34911.1"
                     /translation="METRAAILWEPNTPWSVETITLDPPRAGEVLVEMVASGMCHSDD
                     HARTGDLPAITPLIGGHEGSGVVLEVGPGVTRVAPGDHVVFSFVPACGHCPSCADGHS
                     NLCDNGAALLVGAQLDGTTRHHSADGTDLWTMVCLGTFGEHTVVNEMSCVKIDDDIPL
                     ELACLVGCGVTTGWGSSVYAAEVGPGENVAVIGVGGIGMAAVQGARLAGARQIVAVDP
                     VEFKREQALKMGATHTASSIGEAFELIQEITWGRMCDKVICAMGVGEGQQIAPIMTLC
                     AKRGRVVVTNIHPAEETSIDISLCDLTLMEKQLVGTIFGSANPNSDIPRLMHLYRNGQ
                     LDLAGMVTREYALDDINQGFDDMLGGRNIRGVLRYR"
     gene            46813..48993
                     /locus_tag="PLZ72_01055"
     CDS             46813..48993
                     /locus_tag="PLZ72_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA34912.1"
                     /translation="MTVTEHIADTLVRGSGDGPPAGRPPLFGRLARFSVRRRRWVIAV
                     WLVVVLAAAPLALTLTSGLSGAGWEAQGSVANQVRDELRADFPELGAESAIVVYRQEQ
                     PIVSDPSGLIAVVDQLRGAPGTASVQDPLQLPPEAGMISPDGTTAMVPVHLTGDEDAD
                     LPESAARVIDHVAGIEVPDGARIEVTGEWPVWSDFNASNEEALHKAELLSGLPTIILL
                     LVAFGSAVAAGLPLILAVAGIAVGFASLRLFGMVTPLSVWSMNFSMMIGLAVGIDYSL
                     FIVSRYREEREDGHDAEHAMANTLTTAGKAVFLSALTVVLSLAAVFLVPVMVFRSMAL
                     GMILAVIAVALASLTLLPAVLAAMGDRVLVSRGHDDPDRAAEGRWTRWTQGALRRPRI
                     TLAAGLVLLLALAAPAIGMRLGMPGARVVDEGRSSRDGYEMVVSAFGPGAAAPVFITV
                     AAADSQAVLDVAAADPGAAAASIVARPAESGRTVVRVIPTTAVDSAETTELVSRLRAD
                     VAEVAPGALVGGPAAQNHDLTDVLTGRAPLAIGVIMVVAFLLLLVVFRSLTIALFSIV
                     LNLISVAAAFGFATIVFQHGIGAGLIGIEHQGFVDAWAPLFFFALLFGLSMDYQLFLL
                     AAIRERYEHTGDTATAVREGIARTGRPITNAALIMIVVFIAFGVTGPIPPTELGVTLA
                     LAVLLDATIVRMMLVPSIMGLLGERNWWLPRWMGKRLPIVRFDH"
     gene            complement(49030..49779)
                     /locus_tag="PLZ72_01060"
     CDS             complement(49030..49779)
                     /locus_tag="PLZ72_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA34913.1"
                     /translation="MTRSDAPALEVEGLAFRYPGASGVVLEDLSLRMESGERVALLGP
                     NGSGKTTFVLHLNGLIERQVGSVIVAGLPVQDGNLREIRRRVGMVFQDPDDQLFLQTV
                     RDDVAFGPSNLGLRGAERDQRVDDALAAVGAAALADRNPYQLSGGEARRAALATVLSM
                     QPDLLVLDEPTSGLDPVGRRELAELLVTLGSTQLIVTHDLPFALATCSRAVILDRGRI
                     VADAPTRSLLEDRELLGRHRLEWPIGFDLAR"
     gene            complement(49776..50549)
                     /gene="cbiQ"
                     /locus_tag="PLZ72_01065"
     CDS             complement(49776..50549)
                     /gene="cbiQ"
                     /locus_tag="PLZ72_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNA34914.1"
                     /translation="MSGSHRGNLRRLHVAVDSPVHRLDPAAELVGTVAFAVVVAGTPR
                     HAVAVFALEAAVLVVVVAVSRIGARRVLARLAVVTPFIVFALAIPFVAHGPRTEVGGV
                     PVSTEGLWAAWNVVAKTTLGATAAIVMSATTPLPSMLEGMARLHVPRVVVAIVSSMVR
                     YVELLAAQLARMRTAMVSRGHDPRWLWQVGPVAASVGMLFVRSYERGERVHGAMVARG
                     FTGTLVVSDRPAVATADWVAALAPAAVSAVAVVVWVVGR"
     gene            complement(50573..51583)
                     /locus_tag="PLZ72_01070"
     CDS             complement(50573..51583)
                     /locus_tag="PLZ72_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNA34915.1"
                     /translation="MLVAMHAPDGFLGPGVAVVTAVISIVVIGLALRHASAELDDRQV
                     PLAGISAAFIFAAQMFNFPVAAGTTGHLLGGALAAILLGPWVGALVVAVVVVVQALLF
                     ADGGITALGYNVLNMAIVPAFAGWALFLLWRKVMPRSSGGVVGAAGLACGMTVVLSAT
                     AFSIEWLFGATAPIPFDTVFGAMVGVHALIGIGEGLLSALVVGAVMAARPDLVAGARD
                     LSAEEIGDRRPVGARAFAIGGVLVAIVFAVVVSQFAFDDPDGLERVAQDHGFIDRAQD
                     HAVAGSPFADYATRGIGNAQLSLAVAGLAGVVVTLLVAYGLTTATRRVPPRWSLHTTS
                     SS"
     gene            complement(51656..52096)
                     /locus_tag="PLZ72_01075"
     CDS             complement(51656..52096)
                     /locus_tag="PLZ72_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNA34916.1"
                     /translation="MTTAAVSPPAPPADVDQALQRIRDHGGRVTRAKRTVAILLFDAT
                     DPLSVDDIVERSGLEQSVVYRTLGQFEELGIAEHVHVGHGGAVYRRRGRETVPVGCRV
                     CGRTVELPESEVRSFAARVRDATGIELDLVHFPLSGRCSDCTDR"
     gene            complement(52170..54596)
                     /gene="dnaB"
                     /locus_tag="PLZ72_01080"
     CDS             complement(52170..54596)
                     /gene="dnaB"
                     /locus_tag="PLZ72_01080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNA34917.1"
                     /translation="MSDLSRAPDEPVIRSRIPPHSIDAEQSLLGAMLLSEHAISSVAN
                     IVTEDDFYKPAHRHVFAAIQSLYGAGQGVDPVTVAEELTSAGVLDAVGGPATLVTLQA
                     RTPAITNAEHYARIVEEKALLRRLISTANEVAELGYQPLDDIEKTVDTAESMMFAVAQ
                     RRNADTLQELSPLLDRSLEQLEMLYERGDAITGTPSGYTDLDTMLAGLQPGALIVVGA
                     RPAMGKTALALGIAAHAAVRENLPVLFFSLEMGHLELTQRLIAAEARVDSGKLRTGRL
                     TDADWTKITKAMGRLGEGSLWIDDNPALTVMEIRAKARRLQDRLGTNLGLIVVDYLQL
                     MSGRSSAESRQVEVAEISRGLKVLARELGCPVMALSQLSRQLELRADKRPMLADLRES
                     GCLTAGTRLLRADTNQEVTLGELVSTGERDVPVWSLDHDWKMVPATLTHAFPSGTKSA
                     FRMELASGRTVEATANHPFRTVTGWRRLDELAVGARIAVPRRFGAPERVGTATAADAG
                     ALAERAVADRAVPAEVDAASDAVLAAFLGELFARIGFLGVGSLRGKPLVRLMATSTSR
                     RLIDDVQRLLLRFGVQTRITDVGTRRPRWRVWIHGVDQQRAFLSQIVVPGDRGRDQDQ
                     ALRVLDQITANPNVDTVPCEVRDLVVSELARMEMSQRDLAAALGESYCGGYLLGTESR
                     PRATSRTRLARIADAVDSKELAALADSDVMWDEVVSVTSIGDQPVFDATVLGTHNFVA
                     DGVIAHNSIEQDADVVMFLYRDEVYHPDTQDKDMAELIVAKHRAGRTGIARLVFLDYC
                     TLFANMARTD"
     gene            complement(54925..55380)
                     /gene="rplI"
                     /locus_tag="PLZ72_01085"
     CDS             complement(54925..55380)
                     /gene="rplI"
                     /locus_tag="PLZ72_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNA34918.1"
                     /translation="MTAVKVILRSDVSGLGKRGDIVEVSKGYARNFLAPRSLAFPATD
                     GATAQAEAMRRSRDLKDAKDRESAEEIAKVLVARTIEIPARVGSGGRLFGSVTTTEVA
                     DAVAEQTGIELDRKDLHMDEHIKELGTHHVFARLHTDVQFPITIEVVEG"
     gene            complement(55563..55814)
                     /gene="rpsR"
                     /locus_tag="PLZ72_01090"
                     /pseudo
     CDS             complement(55563..55814)
                     /gene="rpsR"
                     /locus_tag="PLZ72_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(55814..56257)
                     /gene="ssb"
                     /locus_tag="PLZ72_01095"
     CDS             complement(55814..56257)
                     /gene="ssb"
                     /locus_tag="PLZ72_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNA34919.1"
                     /translation="MAGNYVELIGNLTRDPELRFIPSGAAVANFGLAVNRRWRNQQSN
                     EWEEEVAFFDIVCWRDLAENVSESLTKGTRVMVSGRLQQRNWETDNGEKRSKVEVLAD
                     EVGPSLRWATAQVQKTERREGGGFDSAPPPTAPPSGGYDDNEEPF"
     gene            complement(56272..56604)
                     /gene="rpsF"
                     /locus_tag="PLZ72_01100"
     CDS             complement(56272..56604)
                     /gene="rpsF"
                     /locus_tag="PLZ72_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNA34920.1"
                     /translation="MPTRAYELMIIVDQDADDAANRAVIERVKEMVAADGGTTPTVDN
                     WGRRRFAYPINKKNEGTYTVLEIVTEAPNLDEIDRFLRLADDVVRHKLIRLPDQEAAR
                     RGLLSAAG"
     gene            complement(56872..57822)
                     /gene="panB"
                     /locus_tag="PLZ72_01105"
     CDS             complement(56872..57822)
                     /gene="panB"
                     /locus_tag="PLZ72_01105"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNA34921.1"
                     /translation="MAHDSAHDSTASAATSEAAGARAPAAAPAPSRPARITVPWVRSR
                     KVADGADPLVMVTAYDAPGARIADDAGVDMILVGDSLAMVVLGYDDTLSVTVEDMAHH
                     TAAVARARPSALVVGDLPWMSYHVSVEETVRNAAQLIRAGAQCVKLEGGSKRLTMIEA
                     LIDAEIPVMGHLGLTPQSMHAMGGFRVQAKESEAALALVADAKALAHAGCFAIVLEGV
                     PDEVARMVTDAVDVPTIGIGAGPRCDGQVLVYHDLLGLEDRTPAKFVRRYADLKQASV
                     AAVGEFVADVRGGAFPDHSESYHLDSAQAEALSLYGAAVD"
BASE COUNT         8180 a        20767 c        20398 g         7990 t
ORIGIN      
        1 gagcccagga ggagaatcga actcctgacc tgctcattac gagtgagctg ctctgccgtc
       61 tgagctacct gggcgaccgc ctcgtcggcc ggatcgacac gacgcggaca ggcgagcata
      121 ccggaccggc ccgaaagcgg acacggcacg gtccgagcga tccggcctcc ggctcggtcg
      181 tcatcacggt gcggacagct gccggagcaa ccggtcgccg gctcagcgca acgggggcag
      241 cgcgacgaac aacgcctgga gctgcagcga cacgttcggg ttgaaccgca gcgaatcggt
      301 gagctcctgc accgcgtcgg ccgcagccag gaaccgatcg gcaggcgcgc cgtcctcgag
      361 tgcgtcgcgg tagcgggaga ccagggtcgc caggccgctg agcaggtccg ctgtgcggac
      421 ccgacgctgc tcccgcttgt gccgggcgat catcgcgttg cgcggtccgg ccatccgggc
      481 acccagctga tcgacctggg cgtcgaacgc cgcgagctcc tcgtcgtggc gctcggtcaa
      541 cggcgccgcg acctcgtctg tcgcggccag cacctcgtcg accagggcca tggcagtggt
      601 gccggtgccg tcgagtcggt cgggaaccga gtaccaacga tcccggcgga gggcggcgtt
      661 ggggtcgttc gccagcagtc gagcctgggt catcgacccc ccggccccgg ccgcggcgat
      721 ccgggcctgc tcgacgtcga cgccctcgga cacgagccgc tcggcgatcg cgccctgggc
      781 gaccgagtcg aactcgacga ccacgcaccg cgaggcgatg gtgaccatct ccggcgggat
      841 ctcctcggcc agcacgacga ggaccgtcga gggcggcggc tcctccaacg tcttgaggag
      901 cttgggcgac tgcgccgcca ggtgcaggtc gaccagcacg atgacctggc ggtccccgac
      961 gggtggcgcc atgtgggcgc gcctcaccac gtcgtccatc tggtcccggt cgatcgacgc
     1021 cccgacacgc tccaccacgg tgagcgccgg gtgtgcctcg gcgccgacct ggcgacgtcc
     1081 cgacgcgacc gtcgccgggt ctgcgccgtc caacagcagg tccgccgcga aggcccgggc
     1141 ggcttcccgg gtcccggaac cctcgggtcc gaccaacagg tacgcgtgga ccggatcggt
     1201 caccgacgcg cggagccggg ccacggcgac gtcctggccg acgacctcgc cccacaggtc
     1261 cgggccgtcg agatcagtgc cgtcggccgg gccgtccgcg ctcattgtcc cgctcccgga
     1321 accggaccga gctccggcca gcggtcggtc accgcccgca gcacgcgttc ctcgacgagg
     1381 tccgcgtcgc cgtccccgtc gatgcggacc cacagctcgg gagcggccgc ggcctgctcc
     1441 gcgaagctcg ctgccacccg ccggtggaag tccgcaccgg ccgcctcgat gcggtcccgc
     1501 gctcccccgg ttcgttcggc ggcgacgggt tccggcacct cgagcagcac caccaggtcg
     1561 ggggtcagac cgtcggtggc ccaacggttc aggcgcgaca gatcgcccgc gtccagtcca
     1621 cgaccggcgc cctggtaggc gatcgtggag gggatgtagc ggtccgacac cacgtgccgc
     1681 ccggcggcca gcgcaggacg caccaccgtg gccacgtgct gggcccggtc cgcggccatc
     1741 agcagcgctt cggaccggtc ggtgagcgcc acgtcgtact cggccgcggc caacagcacc
     1801 tcgcggagtc gcacacccac gtcggtccct cccggttcgc gtgtgagcac cgccccgagg
     1861 cggctcgcca ggcggccggc ctgggtggac ttcccactcc cctcgccgcc ctcgaacacg
     1921 atgaaccgcc cggtcatgtc ggctcctggg cgtcgggggt cggttccggt tcggctgcgg
     1981 gtccggatcc ggtcaccggg gggttggccg ggggctcggt cggttccgcc tcgggttccg
     2041 aggggagcga cgggtcggga gccgcgacgg ggatggcggt cgggtggttg ccgcgggctc
     2101 gctcctcacc gttcgggccg atcgcctgac ggaggccgtc gcgcagctgg gagcgcagac
     2161 cgatcttcat cgaccggctg gccagcactc cggccccgca gaggatcaga gccgcgagcc
     2221 acaacgtgag gcggacgccg gggatggcca ggtcgaagcc gaagacgctg gcggtgggaa
     2281 cgccttcctg gtcgccggtg gcggcgcggg cgaacccgtt gaagaccgtc gccagggtcg
     2341 gaccgacgac cagcgcgagc agcacgcacg cccggaccag ggtgagcatg gtggcgaaga
     2401 tccggccgcg gagctcgtcg tcggtgtgct cctgcagcag cgtgaagccg agcacgtaca
     2461 ccgctcccgc gcacgccccc agcccgaaga tcccgagcgc ggcgagccag aaggtcgaca
     2521 tcgacacgcc gaagaacatc gacacgccgc cggcgaagac gagcacgggg aaggcctgct
     2581 ccttgggcag ccgcttctgc aacgcggtga gcgacacgac cccggtggcg acccccagtc
     2641 cgagtgcggt gatgaacagc gggaacgtgt tggcgttgcc gatgacgaag cgggcgaagg
     2701 tggggccgag gggcaccagc atcgccccgc cgatcagacc ggtcgccagg ccgaggatca
     2761 cgccgcgcac gatcgggttg gcgccgatga actgcaggcc ctcccggatg tcgtcgaagg
     2821 ccccgcgcag gctcgagcgc tcggcttcct cggcctcggc gaaggtgctg ggagacccgt
     2881 cgccggtcgc cgccgccgcc tcgacggctc gggcttcgcg cgctgcggcg gacgtcgacg
     2941 gcaggccgcg atagacgaag cgcgagatca gcagcgccga caccaggaac gacgccgcgt
     3001 cgaagtagaa cgcgagggtc tgggtgtcgc cgagctcacg gttcaggccc aggaaggcaa
     3061 gcacgctgat ctcggccagg tggtcgttga ggaccttcag gacgtactgg atcggaccgg
     3121 cgatcggcat ggtcgcgtac gcggcgacca gtccgagcga gttggccgtg gtcagccggt
     3181 cggcggggac caccgcgggc accgtcgcct ccttcgccgg cgaccaggcc agggtgaaca
     3241 tctccaggat gaagctggcc acgatcagac ccggcacggt ctgcaccaag ggcagcaaca
     3301 ggaacacccc ggcccgggcg acgtcggaga cgaccatgat ccgcttgcgg tcgaagcggt
     3361 cgacgatcac gccgatgaac ggcgccagga agatgcccgg cgccacccgc gccgtggtga
     3421 cgagggcgat ggcggcctcg ggttgggtcg agatcgacgc cgccagcgag gtgatggcga
     3481 agaagccgac ccagtccccc agggccgaga tgacctggac gttgaagagg gcgaagaagc
     3541 gacgggtgat gaagacccgt tcgatccacc cctgggccgg ggctcccccg gccgggggcg
     3601 gttcgggggt cggtgcgctc aggtcactca ccgccgtccg gtggcggagc cggctcgtcg
     3661 cccccgccgc cggccgagct cttcgtcgcg gcggccttct tcttggccgg gcgcttcttc
     3721 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgctctt cttcttggtc
     3841 gccttcttct tcttggacgg ccccttggcc cgacgctcgg cgagcagctc gagcgcccgg
     3901 tccatggtga gctcctcgac cgaatcgccc ttgcgcaggc tggcgttggt ctcgccgtcg
     3961 gtcacgtacg ggccgaaccg tccgtccttg accacgatcg ggcgctcggt gtcgggatcg
     4021 ggtcccagct ccttcagcgg cggcttgggc gcggcctgac cccgtcgacg cttgggctgg
     4081 gccaggatcg ccagcgcctc gtccagggtg agggtgagga tctggtcctc ggagtcgagc
     4141 gagcgggagt cggtgccctt cttgaggtac gggccgtagc gaccgttctg ggtggtgatg
     4201 acaacgccgt cggcggggtc cgctccgacc tctcgcggta gctccaacag cggcaggacc
     4261 tgctcgaggg tgaccgtggc cggatccatc gacgcgaaca acgacgccgt gcggggcttg
     4321 aggccggtct cgtcgtcgtg ctcgccctcc tggacgtacg gtccgaaccg gccggccttg
     4381 aggtgcacga ccaggcccgt gtcggggtgc tcgcccaggt cgcggtcacc cgacggcgcc
     4441 tcgagcagct cgacggcccg ctcgggggtg agctcgtcgg gaggcgtgtc gtcggggatc
     4501 gacgcccgtt gcacgtcgtc gccgtcgccg cgttccaggt acggcccgta gcggccgacc
     4561 cgggccacga tcggcacccc gtcgccgtcg gccccgagcg gaatcgagtt gatggcccgg
     4621 gcgtcgatgt cgccgaggtc gtcgaccagc cgcttgaggg ccaggccggc ggcgacgtcg
     4681 gcgtcacctg ccggatctcc ctcggcgggc gcgccgccgg caccgccgaa gtagaagcgg
     4741 ctcagccacg gctccatgtc ctccgaaccc gacgcgatgc ggtcgaggtc ctcttccatc
     4801 gcggcggtga acgcgtagtc gaccagggtc gggaagtgct gctcgagcag ggtcaccacc
     4861 gcgaacgcgg tgaagctggg gaccagcgcc gtgcccttgc gccacacgta gccgcggtcc
     4921 tgcaccgtcg agatggtcga ggcgtaggtg gacgggcgtc cgaccccgag ctcctccagg
     4981 cgcttgacca gcgaggcctc ggtgtagcgc gccggcggct gggtctcgtg accggcgacc
     5041 tcgaggtcct cggcgcgcac cgcctccccc accgccaggt cgggaaggat ccgctcctgg
     5101 tcgtcgagcg cggcgtcggg gtcgtcgcgc ccctcgacgt aggcccgcag gaacccgggg
     5161 aacaggatcg tgcggcccga cgccgagaac accgcgacct gctccaccgc agccgacgga
     5221 tcggcgccgt ccacggtcgg cacgacgacc ggcgcggtcg tcgtctcgag gcggacctgg
     5281 accgactcgc cccgggcgtc ggtcatctgc gacgcgatcg tccgctgcca gatcagctcg
     5341 tagagccgga actcggcgtt gccgagctcg ctgcgcacct ggtcgggggt ccggaacgag
     5401 tcgccggcgg gccggatcgc ctcgtgggcc tcctgggcgt tcttcacctt gttggcgtag
     5461 cgacgcggcg cgtcggggac gtacgcgtcg ccgtacttgt cgcggatctg gcgtcgggcg
     5521 gcgttcaggg cggcgtcgga caacgtcgtc gagtccgtcc gcatgtaggt gatgtagccg
     5581 ttctcgtaga gccgctgagc ggtcgacatc gccatcgacg acgacatgcg cagcttgcgc
     5641 cccgcctcct gttgcagcgt cgaggtcatg aacggcggcg acgggcgacg ggtgtacggc
     5701 ttgcgctcga ccgatcgcac ctcgaccggt cggtcggcca gggcgtcgca caccgcggtc
     5761 gctgaactct cgtcgagcag caccacctcg gccgcctgcg ccgatgcggt cagacggccg
     5821 tcctgaccga agtccttgcc cgtcgccagg cggcgtccgc cgagctcgac caggccggcg
     5881 gggaacggcg tcgggtcgcg atcggcatcg acgtggacga acgtggcctg gaggtcccac
     5941 caggacccgg cccggaacgc catccgctcc cgttcgcgtt cgacgacgat ccgggtcgcc
     6001 accgactgca cccgaccggc ggacaggccc cggccgacct tcttccacag gaccggcgac
     6061 acctcgtatc cgaccaggcg gtccaggatc cgtcgggtct cctgggcgtc gaccaggcga
     6121 cggtcgagct cgcgggggtt gtcgatggcg tggcggatcg cctcgggggt gatctcgtgg
     6181 aacaccatcc gacgcaccgg gacacggggg ctgagcacct cgagcaggtg ccacgcgatc
     6241 gcctccccct cgcggtcctc atcggtcgcg aggtagagct cgtcggcgtc cttcagggcc
     6301 gccttcaggt gccggacgtg gtcgcgcttc tccggggaga cgatgtacag cggcttgaag
     6361 tggttctcgg tgtccacccc gaggcgggcc caggcctggc ccttgtactc cttggggacg
     6421 tccgcggcgt tgcgcgggag gtcccggatg tggccgatgg aggattcgac ctggtagtcg
     6481 tcgccgagat acccggcgat ggtccgggcc ttggccggtg actccacgat gacgagcgga
     6541 cttcccacgg ctcggtacaa cacacgcccc gcggaggcct tgtcaaccac ccgagccggg
     6601 tcaccctccc cggagctgcg ctcgacgggc ggtcggccgt gggcccgggg gtagggtgcg
     6661 catcgtcggg caggggcgcc gctcccgtac ctgcgggccg ggcatcgtcc cggttccggc
     6721 accccgcgac gaccccgtcg acccgagagg acgacccatg aacctcgaac cctccgaccg
     6781 cggcaaggcg ctccaggaga cgctgctggc cttcatggac agccacgtgt acccggccga
     6841 gccggtgtac cgcgagcaga tggcggcgtc gggcgacgcc cacttccacc cgccgatcgt
     6901 cgaggacctc aaggccgccg cccgcgagcg gggactgtgg aacctgttcc tgccgcaccg
     6961 cacaccgtgg agccccgatc cgctccccaa cctcgattac gcgtacctgg ccgagatcac
     7021 cggccgctcg accgcgctgg ctcccgaggc gctcaactgc gcggcacccg acaccggcaa
     7081 catggaggtg ctcgccctgt tcggcacccc cgcccagcag gaggagtggc tggtgccgct
     7141 gctcgagggc gagatccgct cggcgttcgc catgaccgaa cccgacgtcg cctcctccga
     7201 cgccaccaac atccagatgc gcatcgagcg cgacggcgac gactacgtcc tcaacggccg
     7261 caagtggtgg acctcgggtg cggcgtcgga ccgctgcaag atcatgatcg tcatgggcaa
     7321 gaccgacccc gacgcccccc ggcacctcca gcagtcgatg atcctggtgc cgatggacac
     7381 gccgggcgtg accaaggtcc gtgacctgcc ggtgttcggc taccaggacc gcgagggcca
     7441 cggcgagatg ctcttcgagg acgtccgggt gccggcgacc aacctgctgg ccgaggaggg
     7501 ctccgggttc gccatcgccc aggcccgtct gggaccgggt cgcatccacc actgcatgcg
     7561 ctgcatcggc atcgccgagc gcgccctcga cctgatgtgc cgccgggtga ccgaacgggt
     7621 ggcgttcgga cggcccctct cggagcaggg cgtgatccgc gagtggatcg cggactcccg
     7681 catcgagatc gaacaggccc gactgctcac gctgaaggcg gcccacctga tggacaccgt
     7741 cggccacaag ggcgcggcga tcgagatctc ggcgatcaag gtggtggcgc cgaacatggc
     7801 gctgcgggtc gtcgaccggg cgatccaggc ccacggcggc ggcggcgtgt ccgacgactt
     7861 cccgctcgcc cagatgtacg cggggctgcg cacgctgcgc ttcgccgacg gtcccgacga
     7921 ggtgcaccgc cgtcaggtcg cccgtcagga gctcgcccgc cacacctgat cgctcccggc
     7981 tccggccgga cgccgggccc cggtcaggtc ggcaggtcct cctgggcggc gagaacctcg
     8041 agcaggcggt cgacgccgag ctctaccagc aggtcccgcg ccggaccggc cgggcacacc
     8101 accgcgaagc gacctccggc gcgccgggcg cgcagcgacc cgacgaacag caccccgagc
     8161 gtgaccgggt ccaggtggtc gaccccgctg aggtcgaccg ccacgtcggg cgattcggtg
     8221 cggtcgagcg cctgacgaag cgccgggatc gtcgccaggt ccatgtcgcc gaccacgcac
     8281 agcaggtccc accggcccgc cgggacgatc cgcacgtcga acagcacgcc ctcaaccgta
     8341 ggtgccgggc gcccgagcgg gttccgtcac tgccctgacg tagcgttccc accacgatga
     8401 ccactcggtc cgacccctcc gagacccccc agggcccggc cgtcggtgac accgacgact
     8461 gcgtccggga caccgtcgac gccctcgtcg ccgagtggtc ggccgagccc cgactggtcc
     8521 atgtggaacg gctgtcgggg cgaccggccc gtctggcacg cccggcggaa ccgctgcacc
     8581 cggcactcga ggatcgtctg ggcgagcggc cgctgtggtc gcaccaggcc cgggcgatcg
     8641 acctggtccg ctcgggtcgc tccgtcgtgc tgtcgacgcc gaccgcgtcg ggcaagtcgc
     8701 tgtgctacca ggtgcccgcg gtcgacgcgg tgctggaacg tggggcgtcg acgctgatgg
     8761 tgttccccac caaggcgctg gcccaggacc agctgcgcac cctgaccgga tgggacctgc
     8821 ccggcgtcgt cgcggccacc tacgacggcg actgcacccc cgaggagcgg acctgggtgc
     8881 ggggccacgc cgacatcctg ctgaccaacc ccgagatgct gcaccacggc atcctcccca
     8941 accacggccg ctgggccacg ttcctgcacc ggttggagct ggtggtgatc gacgagctcc
     9001 acgtcctgcg cggcgtgttc ggcacccaca ccgcgcaggt gctgcgccgg ctgaggcgnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg agccggcggc gacgggaccg
     9181 atcgacgagc tcgagcagat cgaggacgcc gccgacggcg tcgatcgccg gacgtcggtg
     9241 cacgccgagg ccgcgacggt ggccgcccgc ctggtcaccg ccggactgcg gacgctggtg
     9301 ttctgccggt cgcgcaaggc caccgagctg gtcgccgcag agatccgtca ccgcctcgac
     9361 cccgagcacg cggacggtgt gcgggcctac cgcgccggct acctggtcgg cgagcgccgt
     9421 gacatcgagg acgacctgtt ctcgggacgt ctccactgcg tggtggccac ctcggcgttg
     9481 gagctcggcg tcgacgtcgg cggcctcgac gcggtggtgc tcgccggcta tcccggcacg
     9541 gtcgcctcgt tctggcaaca ggtcggtcgg tccgggcgga gccgtcggcc gtcgctcgcg
     9601 gtgctggtgg cgggtgagga ccagctcgac cagtggatgg tgcgtcaccc cgacgacctg
     9661 ttctcccgag cgccggaacc ggcggtcatc aacgccgaca accccttcgt gtacgtcccg
     9721 catctggcct gcgccgctca ggagcaggcg ttgcgtcgcg acgacgagcg catgtggccc
     9781 gaccagctcg acgagggggt ccgacgcctg gtcctcggcg accgcgccgc ggtgcgacga
     9841 cgccgcgggc agcgggaggt ggtctggacc ggacggggcc tgcccgcccc gacgatcggg
     9901 ctgcgttcgt cgtcgcgggg cgaggtccgc atcgtcgacg gcgacggcga gctgatcggc
     9961 accgtcggcg ccgaccgggc gcccgccacg gtgcacaccg gagcggtcta cctgcaccag
    10021 ggtcggccgt actcggtcac cgatctcgac ctggacgccc gcaccgccgt cgtggaaccc
    10081 acgagcggcg acgcgtacac ccgaacccga tccgagacct cgatccgggt gctggagcgg
    10141 gaccgccacc gcgtggtcgg tgccgcgggg ctggcgctgg gcagcgtgga ggtgacctcc
    10201 caggtcgtgg gcttccagac ccgcagcgtg gccacgcacg aggtcctgga cacgacgccg
    10261 ctcgacctcc cgccgagccg cctggtcaca cgggcggtct ggtacacctt cgacgacgac
    10321 ctggtcggcc ggtcggaggt gggtgatcgg gagctgcccg gagcgctgca cgcgttggag
    10381 cacgcggcga tcgggatgct gccgctgttc accatctgcg accgatggga cgtcggcggg
    10441 gtctccacgg cgtggtcccc cgacacggcg tcggcgacgg tgttcatcca cgacgcccac
    10501 gagggcggcg ccgggatcgc ggagctggcc ttcgacgcgg ccgaccggca cctggccgcc
    10561 accctcgagc tgatcgacgc ctgtcggtgc gcgacgggat gccccagctg cgtgcagtca
    10621 cccaagtgcg gcaacggcaa cgaaccgctc tcccgggacg gggccgtgcg actgctccgc
    10681 gccacgctgt gctcgaccga caccggcgcc ggctccggtc cttcggtcga ctccgccatc
    10741 gacgaccgcc acgcatcctg accccgggcc tggatcgggt cacgccccct ccacccgaac
    10801 cgccagcgcc tcgctcagct cgattccgcc gaccagtcgt ccgacgaccg gtaccgacgt
    10861 cggacgggtc gacacccgga cccgggcgac cccgccggga gcggtgtcac caccgatctc
    10921 gacgcggaac cggccctgtc cgacgtcgcg cagcgacccg cgcacggagg ccgggtcggg
    10981 atcgaccgct gcggcccgcg ccgcggtgcg cgccgcgtgg acggtgagga cccggtcccg
    11041 ggccagcagg ccgacctgca cgaccagcag gagggtcgtg accagcaccg gcagcagcat
    11101 cgctatctcc accgtggcct ggccgcgggc gtcgggcgat gcccaccgat cgggccgcct
    11161 cacgctcatc cggcctggga caggatccgt ccgacgatgg cgtcgaacaa ccgcgacacg
    11221 gcgtcgctgc gcgtgaccca ggcgaccagc gcaagggcca ccgcggcggc cccgagcagc
    11281 accagcgcgt actcgacggt gctctgggcc tgctcgtcga ggacgcgctg acgggtgtac
    11341 cagcgcgccc acagggcggc gccgggacca cccgatcgcg gacgggacgc tcgggagcgg
    11401 gtcgggccgg cacgaccggt gtcggagtgg tcggggtacg ggtgggacat gggaacggtc
    11461 acggtggtcc tcctcggggg acgaacggtg cggaaggggg aagcgggaac ggcccggcgg
    11521 acgcggtcgc ggctccgagg agcgcaccgg tcccgcgggc ggcggacagc gcgaccggga
    11581 ccacggtcag catcacgaag gcgggcagca cgagagcgac cagcgggatc aacagcagaa
    11641 ccgggagtcg acggacccgg gcctcgatcc gccgtcgtga ccgccggcgt tcggcgtccg
    11701 caaggcgcac cagcgcggcg gcgggcggcg tacccgacga cgccgcggtc gacagcgtgg
    11761 tgaccagcgg ttggccggcc ggtcccagct cgtggcgcaa cgaggcgatc acgtcggcca
    11821 gcaccttccc gcgctgcaga tcggcaccgg cgcgcccgag cgccgaagcc accgggccgt
    11881 cgccgacgtc gcccacggct tcgaccgatc ccgccaccgt cgagcctgcc gcggcagcca
    11941 cggccaggag gtcacagcag tgcgccatgt cccacagctc ctcgacggac cgtccacccg
    12001 cggttggccg ggccagcggt cgctcaggac tgcgttcggc acgcggtcgg gcggtctgca
    12061 gtcgagcgac gaggatcgcc cggaacgccc ggtcgaccac cgccgtggcg gacgctctcg
    12121 tcggctcggc ccggaccgcg gaccccggct cgtcctcggg gaagcgtcgg gggcggggcc
    12181 gcgggacgac cagatcggcg accgcgacag cagccgccgc ggcgagcagc cacggcatca
    12241 acgggcccat cgacggttcc tccgtcccga tccctggacg cccggcgcgg ccacggcccg
    12301 ggtgacgaga cggttcgaca ccactgctcc gagcccatcg agcacgactc cgaccaccag
    12361 gcacgcccga cccgccccgc tgcccaggac cacccccgcg aacgacgggt cgacggccgc
    12421 gaggaccgtg acgccgatca ggggcagggc gacgagcacg gtcgccgagg tccgggcctg
    12481 gctcgtcagc gcccgcacct cgtcgtcgag ctcgatgcgg tcgaccagcg cggcggcgac
    12541 gccgtcgagc gccgcgacca gggccactcc acggccgcgg gagaaccggc aagccgccac
    12601 caggagctcc accccgtcga tccggctggc ccgaccccac agctccatca cccggtccac
    12661 ccccaggccc cggtcgagca gcgcgaccgc gccggccacc tcggtgccga gcagcccgac
    12721 cggtcccccg gcgagctcac gcaacgccac ggtgagggtc gcccccgatc cgaccgagcg
    12781 ggccaggtcg cccaccgcgc cgggcaggtc ccgggccgcc cgggtggcga gacgtcggcg
    12841 gcgacgccgg cccgacccgg cgagggccac accccccgcc gtgatcccga cgacggccag
    12901 gaccagcagc gtcatgtcac cggcaccagt cgaccgtcgg agccgaggtc gtgcagcgac
    12961 gcgacctccc gacggccgtc ggggcgccgt tcgactccca ccagcgtgtg cagagccgcg
    13021 tgcacctgct ggcggacgac gtcgacgggg gcgccgtcgg caccggcgac ggcgaacgag
    13081 gtgaggcggt cgacggcgtc ggacgcggtc ggggcgtgca cggtcgacat cgagccgtcg
    13141 tggccggtcc cgagggccca caccatgtcg gctgcttcgg cgccgcggac ctcaccgacc
    13201 acgatccggt ccggtcgcag acgcagggcg acccgcacga ggtcgcggaa gtccgcccgc
    13261 cccaccccag ccgccccacc cgggcgggcc tccaggcgga cgactccccg accggggagg
    13321 cgcagctcgg cgacgtcctc gacggtgatg atccgttcgt ggtcgggcag ctcggaggcg
    13381 agcgacgcca acagggtcgt cttgcccgat cccgtcgctc cgaagaccac caggttccgc
    13441 cgaccgacca gacgttcccg aaggagtgct gcgacatcgg gtcgggcgag ggagtcgagc
    13501 gggatcgggt cgcgccggtg gcgtcggacg gccagcaccg gtccgtcgac cgcgagcggg
    13561 gggagcaccg cggtcacgcg ggtgccatcg gccagtcggg catccacgac tgggtgggcc
    13621 cgatcggcgt gcagtccgag cggtccgacc agtcgctcga tcgcccggtc caccgcggcc
    13681 cggtcgagca cgatcccggt cgtctggagc tgaccggcac gctccaccca cacctcgccc
    13741 ggaccgttga ccagcacgtc ggtcaccgtc gggtcgccca acagccgttc gaccgcgccg
    13801 aggccgacca ggtcgaccgc cagggcccgg gccgtctcga ccaccgccgc gtcgcccatc
    13861 agcggacgtt cctcccggac gatgcgggcc agccgtccga cggcgtcgtc gccccggtcg
    13921 ccgtccggct cggagcggaa ccgtcgaccg acgaccgcga cgagctcggg atcggtgcgg
    13981 gccatggtgg tcacgggcgg gactccccga tctcggccag cggggcgaga tcgcgggcga
    14041 gaccccgcgg cgggcgtgac gagagcagac cggcgtcgac ggcccgggcc accgtcgggt
    14101 cccagtccag ctcgtgcacg gggatcccga gggcccggga cacatcggtg ccgtcgagag
    14161 cccggcccgg ctcgaccacc aggacggcgc tgtccgctgt cacctgtgag cgcaccgcgc
    14221 gccgcagtgc caggtagcag gcccggacga cgaggaccga gatcccctcc tcgcacagcg
    14281 ccgagcgcag agccgaaccc ggcgaccgta cgcccgcgtc gacgaccacg gtccgtcggt
    14341 ccgagcgcag cacctccacg aggtcactga cccgctccgc cccgatcgct ccgttcaggt
    14401 ctccgggcag cagccgcagg ccgtcggcga cctcgatctc cagtcgtccg agcgccgcgg
    14461 cgtcaccgtc gggggagccc aaccagtcac cgacgccggg gccggtcggt tccggaatcc
    14521 cgagcagggc gggctggtcg cccgccaggt cgaccagcag gacctcgcga ccccgctggg
    14581 tgaggagggt ggccaggccg gcggagacca cggaggtccc cgacccgccc ttgaccgacc
    14641 acagggtgaa cagcacgcgc gcgacctcct gacatgcccg ccgtgggcgg gccgtgaggg
    14701 ggaagcggct ccggaggaga ccgacaccgg ttccgggccg accgatgcgg tcggcggtga
    14761 ggtgtcgtca ggaccctacg acggatcagg gtcgtcgacc accggggacc gcgacccggt
    14821 cgcggtcccc ggtggtcctg acgtcgctca gatcagacca ggagcaggtc ccgagccccg
    14881 gactcgttgg aggggtggcg gagcttcgac atggcccgga cctcgatctg acggatccgc
    14941 tcgcgggtca gctcgaactg cgcgccgacc tcctcgaggg tccggggttc accccggtcc
    15001 agcccgtagc gcagtcgcag cacctcgcgc tcgcgctcct cgagcccgga gagcaggtcg
    15061 tcgaccgctg tggggaccat cgcgtgcagc gccgcgccct cggccgtcgg agcatcgtcc
    15121 gcgaccaggt cggccaggac cgcgtccgcg tcctcccgga gcggttcgtc cagcgagagg
    15181 ggctcgaccg cgtacagaag ggtctcggtg accttgtcct cgggcagatc gacctcgcgt
    15241 gccagctcgg cggtggtcgg catccgaccc agctcgatct cgagtcgcga acgcgtccgc
    15301 tggacccgag tcatcgtgtc gcccgcgtgg acggggatgc ggatggtgcg gccggtgttg
    15361 gcgatgccgc gggtgatcgc ctggcggatc caccaggtcg cgtaggtcga gaacttgaag
    15421 cccttgcgcc agtcgaactt ctccaccgcg tgcatcagcc cgaggttgcc ctcctggatg
    15481 aggtcgagaa gcgggacccc tgagctctgg tagcgcttgg cgatcgagac gaccagacgc
    15541 aggttggcct gcacgaactg ctcggccgcg gcctcgcccg cccgcacccg acgttgcatc
    15601 tggcggcgtc gggtcgcagg gatggcggcg ccggatccga gctcgcgggc cgccgccgca
    15661 ccgtcttcca tgacgcgggc caggcgagct tcgtcgtcct tggtcagcag gtcgtggcga
    15721 ccgatctcgg ccaggtacct acggaccagg tcctcgtcgt cgcgctcccg tcgctggtcg
    15781 gtcaccggca cctcgccttc cctcggatcg cggcggtcga cccgaccgcc gggtccccgg
    15841 gacgtgtcgc tcccagtgga cctcacgtca tcggcacggc agggtcgaaa acctgagcgc
    15901 gatccgacac gcaccggcac gcgccctcat ccgctcgtgg gacccggggt ggttcaggcg
    15961 gtgaacccgc aggtggcctc gagcaggtcg agcatctcga caggcggccg atccggtccg
    16021 acccgggtgc ggacgtccgc cgcatcggct cggaggcgat ccacctgggc cggcagtcgc
    16081 gccagcagcg gtcggtccgc caccggggtg caccgcaaca ggtgtgtgtc gtaggcgtcg
    16141 gccagggctg acgcgacgac cacccacgcg gcgaggcggt cgccctggct cagcgctccc
    16201 agcagctcga gcacggcgtc ggcggcgggg ccgggtgaac gcacgagcgt gtcccggggc
    16261 agctcccgca gcctcggcag gcggtccacg aggagcgcgg aacgtgcggc gtggcccatg
    16321 gcgagccggt cgagcacgac gacgacgtgc gcgccgtccg cgtcgggcgc ccacccgccg
    16381 acgatccggt gcaaccgggc ttcggtccac gcccaggacc cgagccggcc ggcagagctg
    16441 agcaggtcga gcggacccga gtcgtcccgg aactctcccg cgacggtcga ggccggtggg
    16501 tacccgtcga cgacgtctgg cgtcaccacg tcaggccgtc ccggagtcgc gggtggtgcc
    16561 gttggtgatc ggcaggcgac ggtcgcgccc gaaggccttc tgggagatcc ggatgcccgg
    16621 cggcgactga cgtcgcttgt actcggcgag gtcgaccagg cgggtgatgc gacgcaccat
    16681 cgcgtcatcg tggccggcgg cgaccaggtc ctccaacgtc tggtcgccgt cgacgtaggc
    16741 ctcgatcagc gggtcgagca cctcgtaggg gggcaggctc tggtcgtcac gctggtcggg
    16801 ccgcagctcg gccgacggcg ccttggtcag gatcgattcg gggatcagcg cggcgccctc
    16861 acgctcgttg cgccaccggc acagctcgta gacgagcagc ttggggatgt ccttgatgac
    16921 ggcgaggccg ccggcggagt cgccgtagag cgtcgaatag ccgacggcgg tctcgctctt
    16981 gttgccggtg gtgaggacga gccacccgaa cttgttggac agggccatca gcagcacgcc
    17041 gcggacccgg ctctggaggt tctcctcggt gaggtcgggc ggtcgctcgc cgaagctggg
    17101 ggccagcagc tccaggaagg cggcatgggc cgcttcgatc gggatcgtcc gggcgtcgac
    17161 acccaggttg gacgccaggt ggtgggcgtc cgcgagcgag tggtcgctcg agtaccgcga
    17221 cggcatcagc acggcgtgga cccgttcggc gccgacggcg tcggccgcga tcgcgctgac
    17281 cagcgccgag tcgaccccgc cggacagccc gagggtgacg tcggtgaacc cgttcttggt
    17341 cacgtagtcc cgggtgccga gcaccagggc gtcccagatc tcctcgggcc cggccggcga
    17401 cggctccacc agggcgtcta ccggggtgag ggagtcgcgc ccgggggcgg gttcgctgac
    17461 cgcgaggacc gggccgaccc cggagggccc gtccaccgcg acgtcggcgc tgacatcgac
    17521 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacgggcc cgggcctgga
    17641 cctcgccgcg gtcgacgacg aacgatccgc cgtcgaagac cagctcgtcc tggcccccga
    17701 ccaggttgac cgccacgatc ggcaccgagc actcggcctg gcggtcgagc agcgtcgact
    17761 cgcgcaactc ctgcttgccg gcgtggaacg gcgacgcgtt gatgttgagc acgacgtgcg
    17821 caccccggcg ggtcgcgtcg agcgccggtc cggccggtac ccacaggtcc tcgcagacgg
    17881 tcaccgcggc gagcaccccg gcgatccgga acagcggctg accggcgctc cccggatcga
    17941 agtagcgctt ctcgtcgaac acgccgtagt tgggcagggc ctgcttgcgg tagctgccga
    18001 gcaccctccc gtcgtgcagg accgcggcgg cgttccaggg gccgtcggtg gagtcgtggg
    18061 gatctgctcc cggggtgcgg tgaccctcga cccagccgac gaccgccgcg cagtgcccgg
    18121 tgacctgcgc ggccaacgag tcgagagcgg cggcgttgtc ggccacgaac ccgggccgca
    18181 gcagcaggtc ctcgggtggg tagccgcacg tggccagctc ggggaacagg gcgacgtcgc
    18241 aacccgcggc ctcggcgatg cggtaggcgt cggccatggc ggccacgttg gagcgcagag
    18301 cgccgacggt caggtccagc tggcacaacg ccacccgcag cacgacggcc ggtccgggcg
    18361 cggtggaggg ttcgggcgtc acgagcggat ggtacccagc gctgcggacg cgccgagggc
    18421 ccggcctggg ccgggccctc ggtcgcggat cggagcgtcc cggtcaggcc gacgcggcgc
    18481 cgcagaccgt gttggtcatc aggcgggtca cgacgtacgg atcgatgttg gcgttcggtc
    18541 ggcggtcctc gatgtagccc ttctggtcgc gggcgacctg ccacgggatg cggatcgagg
    18601 cgccacggtc accgacgccg tagcggaact ggctgaagtg cgccgtctcg tgctggccgg
    18661 tcagacgatc ctcgtagccg tggccgtaac cggccaggtg ctcctcgacc ttgccctcgg
    18721 cgccgagcgc ctcgcaggcc gcgatgatgg cgtcgtagct ctcgcgcatc tggcgggtcg
    18781 agaagttggt gtggcagccg gcgccgttcc agtcgccctt gatcggcttg gccgagatgg
    18841 acgcgttcac gccgaagtcc tcggagatgc ggtagagcag gtagcgggcc atccagatct
    18901 ggtcggccac ctcgacggtt ccgcacgggc cgatctggaa ctcccactgg ccgagcatga
    18961 cctcggcgtt ggtgcccgag atgctgagcc ccgccgccat gcaggcgctg gtgtgggcct
    19021 cgacgatgtc acgaccggcg atctcgaccg cgccgacgcc gcagtagtac ggaccctgcg
    19081 gggcggggta gtagtagccc tcctggggcc agccgagcgg ccggccgtcc ttgaagaacg
    19141 tgtactcctg ctcgagaccg aacagcggct cgaaatcggc gaacttgtcg gcgatctcga
    19201 cgcacgcggc acggttgttg gtggagtgcg gctcgaggct ctctggcagg agcacctcgc
    19261 agagcaccag cacgtcgttg ccgccccgag tggggtccgg gacggtgtac accgggttga
    19321 gcatgcagtc ggagttggac ccctcggcct ggttggtgga ggagccgtcg aagttccaca
    19381 gcccgggctc gtcgccgtcg gggacgatct tggtcttgga gcgcatgagg ggggtcggct
    19441 ccgtgccgtc gatccagatg tactcagcgc gatacgccac gttcgcttcc ttctgtcgtc
    19501 ggatccggcg ctggccggtc ggggcccacc gggcccggtg accgccatgt tgccccacgc
    19561 cgttttcact cccgttgccc gattgtgaac ggcgtgtgaa ctgagcccgg tgtgccggga
    19621 accccggccc gtcggctggt accacagctg cggacggtcc gtcggcgatc ccgtgctcgt
    19681 cggccgaccg gatccggatc ggcttgggtg cccgccggcg cccggtgaca cgatggagcg
    19741 gtcatggacc accaacagga ctacgtgctg cgcaccgtgg aggagcgccg ggcccgcctg
    19801 gtgcgactct ggttcaccga cgtgctcggc cagctcaagt cggtggcgat ctcgccggtc
    19861 gagctcgagg ccgcgttcga ggagggcgtg cagttcgacg ggtcggcgat cgacggcttc
    19921 agccgcgtgc aggagagcga cgtcctggcc ttccccgacc cgaactcgtt ccagctgatg
    19981 ccggcacggg acggcgagga ggtgaccgcc cgcatcttct gcgacatcac ccgcctggac
    20041 ggcgcgccgt tcgagggcga tccccgccag gtcctcaagc gaaacctcgc ggcggcgtcc
    20101 gaggccgggt tccagttcat gtgcgccccc gaggtcgagt tcttctacct ggccagcgac
    20161 gacccccggg acccccagcc gctcgactcg gccagctact tcgacctcac cacctacgac
    20221 gagacctccg acatccgccg gcggaccatc caacgcctgg agggctcggg gatcccggtc
    20281 gagtactcgt tccacgagga cgccccctcc caacacgaga tcgacctgcg ctacgcggat
    20341 gcgctgacca tggccgacga cgtcatggcg ctgaggatga tcgtgcggga ggtggcgatg
    20401 gcgtccgggg tgcacgccac cttcatgccc aagccgttcg agggcgtgca gggatccggg
    20461 atgcacacgc acctgtcgtt gtggagcggc gacgacaacg ccttccacga ccccgacgac
    20521 gcggtcggcc tcagcgccac ggcccggagc ttcatcgccg ggctgctcgt ccacgccggc
    20581 gagctcaccg cggtgaccaa ccagctggtc aactcctaca agcggctggt ctccgggttc
    20641 gaggcgccgg tgcacatctc ctgggcacgc aacaaccgct cggccctggt gcgggtcccc
    20701 gttcccaaga agggcaagga ggaccagggc acccggctgg agtaccgggc gctcgacccg
    20761 gcctgcaacc cgtacctggc gttctcggtc ctgctcgccg cgggcctggc cggcatccgc
    20821 cacggctacg agctgcccga ggagacgacg gtgaacctct tcgagtccgg cgtcgatgcg
    20881 cccgccgacc tcggacggcg cctcccggcc aacctggcgg aggctctcga cgctatggag
    20941 cgctccgagc tcgtccgcga ggcgctcggc gagcacatct tcgagtggtt cctgcgcaac
    21001 aagcgcgccg agtggggcgc gtaccgcacc cacgtgtcca gcttcgagct caaccgctac
    21061 ctgaggacct ggtaggggaa cgtgcagccg ttgctcgtct ggcccgaccc cgccccgccg
    21121 gacgtggccc gggtcctcga cctggctgga ctgccgtggc gcgccgtcgc cgacgaggcc
    21181 gtggcacgcg acggcgtgtg gtccggcgcg gtcgtggccg ccgacgagga ccctgagcgc
    21241 ggcttcgcca tggcgcgaac gctgcgacgg ggcgacctgg ccgtcgcccc gctcttgttg
    21301 gtggtgcgcg gcacgcagct ggccgacctc gacgggcgcc acgacacctt cgacgacttc
    21361 tgcctggccc cggcccatcc gaccgagctc gaggcccggg tgcgtcacct gctggccgcc
    21421 gagccgggac tggcgctcga gccggacgtg gtgcagtacg aggcgctggt gctcaacctg
    21481 gagacctacc aggcggcgat cgacgggacg ccgttggatc tcacctacat ggagtacgag
    21541 ctgctcaagt tcctggcgtc gaacccgggc cgggtgttca cccgcgagat cctgctgtca
    21601 cgggtgtggg gctacgagta ctacggcggc gcccgcaccg tcgacgtcca catccgacgg
    21661 ttgcgggcca agttgggcga ggagcacgcc gggctgatcc agaccgtgcg gtcggtcggc
    21721 tacaagttcg gcgagtcgcg ctgggggtcc tgagcggttc tcggccgcgg ctgccgggac
    21781 cggcctggcg gcgccgttcc cgggcctcgc cggtcagacg ttgaggtcga tgagctcgct
    21841 gccctcgttc aggtactcgc cctctttggc caacgactgg ccgaggatca ccgagatcac
    21901 gcctccggtg atgatgaagg cgatgggcag cgcgaccacc aggaccacga tgatgagcag
    21961 cggaccgatc atggcggtca gtctgcccca gtgaagaccc aggcctccac ctccacccgg
    22021 gcgccgagcg gcaggcctgc caccgccacg caggtgcgcg ccggacgggg ctcggtgaag
    22081 tagccggtgt agatctcgtt catctcggcg tagtcgtcca tgtcggccag gaacacggtg
    22141 gtcttggtga cctggtcgga gcgggcaccg ttcgcggcga gcagttcgtc gaggttggcg
    22201 agggcctggc ggagctggcc ggcgaagtcg tcggccagcc cgtcggggcc gaggccgatc
    22261 tgacccgaca gcaccagcca gtcgccggcg cgccgggcgg gcacgtaggg agcggtcatg
    22321 ggctcctgtc tacccgatcg cgcccccgcc gaacttgaag cccccggtca ccctcacgac
    22381 ccaccagcta caagttcgac gcagcaaccc gaacttgcag cccccggtcg ccccagcgac
    22441 cccacagcta caaattcgat aggaggccgg cgggccagtc gggcggggtg cccaggaacc
    22501 gccacgtcgc cgcggccacg gcggcgaaca ccgcgtcgga cactcggacc gggggtcggt
    22561 ccgacgggac gagctcgacg gtgatcgctg gggtgtcggc cgccttgagg accccgaggg
    22621 agcggacggt caggtccagc accgaaccct ccgcgtcgac ggcgacgccc tcggacgtca
    22681 cccagccgaa cgccatgtgg gccgctccga tgcaatagga gcgcagaacg accgggtcga
    22741 ggatgtcgcc accagcggcg cggacgcgga tgccgtcggc accgatcgac gcctcggcca
    22801 ccgcgccctc cgaggtgcgg accaccaccc gctcgccacc ggcctggtcg ccgacgcgtc
    22861 cggactccac cgagaccacc tcgctcggag caccgacgcc ggcgacgagg acgaccgcct
    22921 cggcccaccc cgctccgcgc agcgtcaccg acgtaggggg tccggcgacg tcgagctcga
    22981 ccacctcgag tccgggagcc gcggcacgga tccgctccgc gatcccttcg gtgcgcacga
    23041 cgtggatcac gcccgtcccg tcggcgcgga tgcccgctgc caccggcggg cgcttgggcg
    23101 ccgaacgact gcagtcctca cgcgaccatt gggccagcac cgctcgaccg tgctcgtcgg
    23161 ccagtcggcg cgccacggcc gggagcgggc tgtggcgctt ggccccgaac gccccgccgt
    23221 tggccgcggc gggggacggg tcgccgcccg gaacgcacca cgacgagtcg gtctcgacgt
    23281 aggcggcgtc gacccacccg gtgcgcaacg tgaggtccca gtccccgtcg ggcacctcga
    23341 gcgggggcac cggatcgacg gtggtgcgcc gaccctggac cttgccggcc gcccgacgcg
    23401 cctcgaccag ggactccccc accacccagt cgtcggaacc cggcaccggc accgcgatca
    23461 gcgcatcggc gggcgcggtg tcggccgaga agccgaccgc cccgaggacc acccgctcgc
    23521 ccacctgctg gggggcaccc agctcgaggg tcgcccgccg cgccgcggcg tcgagatccc
    23581 ggggaccgac ctcgaccggc gtcgatccgg acagcacgtc ctcggccgcc tcgacgatcg
    23641 tctgccatcc ggtgcagcgg cacaggtgcg cggccagggc tcggtcgacc gctccggggc
    23701 gcacgtcgcc acccttggcc cgcacggcgt cgaggcggac gatgatgccg ggagtgcaga
    23761 aaccgcactg actggccccg tgctccacga aggcgtcgac ccatcgcgac cgtgcctcgg
    23821 gctccaggcc gtcgagggtg acgaccgacc gcccggccac ccgccgcaac ggcgtcacgc
    23881 acgccacccg cggggtgccg tcgaccagca cggtgcagca tccgcactgg ccctgggggg
    23941 agcagccgtc cttgaccgag tggaccccga gcgggccgcg cagcgcgtcg agcagggaga
    24001 tcccgtcgtc gacgacctcg acgtcacggc cgtcgacggt gaggccgacg gcgcgggtcg
    24061 ggacctcggc ggacggctcg tcaggcgacg gggacgcgtt catggtcggc tcctgggggt
    24121 tcgggcccgg gggtcggaga cctgacggga agacgttccc ggtcgatgtc acccggacgg
    24181 atcgagacgg taccgtgcgc cgtcgtggcg tcccaccctc acccccgccg ccccggcgcc
    24241 ggaagcgacg ctcccaccgc gggcccgagt cggcgttccg tcctgcgggg cggggtcggc
    24301 ctgggcgcag ccaccgtggg cgggatcctc ctcgggtcct gcagctccga ggcagacacc
    24361 cgcagcgccg acaacctctc cagccacgcg ctcctggccg ccttcccaca gaccctgccg
    24421 cacatccccg ccggtgttcc gttccggttg ccgtacctga tctccgacga cgagggcgtg
    24481 ccgctcacca ccatcgacgg tgccgtcacc ttcaccgtct cccgcgacgg ccacgagttg
    24541 ggcacggtcc gggtcgagcc ccacagcgac ggcgtccccc gtgcgtacct gccggtcggc
    24601 ttcaccttcc ccgagcccgg tcgctacgac ctgacggcca cctacgacgg tgccccgatg
    24661 tcgtcgacgg tcgaggtggt cgagcccgcg cgggtcggtc cacccgtcgt gggccagaag
    24721 ctgccaccgg tcgactcgcc caccacggag cggaccctcg aggtggaccc gctgtgcagc
    24781 cgcctgaagg ggccgtgtcc gctgcactcg gtgaacctcc gcgacgcggt tggcctgggg
    24841 aagccgatcg tggtcgtcat cgccacgccg gcgtactgct cgagcgacgt gtgcgcaccg
    24901 ctgctggagc tggtgctgga gcagaccgcg gggcgcgacg acctcgtcgt cacgcaccag
    24961 gaggtctaca agaaccccaa gggtcagagg gacctgagcg acgcacagct cgcgccggtg
    25021 cccgacgcct accgcctccg attcgagccg gtcctctacg tgacggaccg gaccggcacc
    25081 atcgtcgccc gcgccgacat cacggtggac cggggagagt tgagcgagct gctcgccctg
    25141 gcggtgtgac ccgccgacca gcccgatcgg gccgcgggcg gcgacgcccc ggccgcctcg
    25201 ctcagctgaa ggactgaccg cagccgcagg agctctgcgc gctggggttc gtgatggcga
    25261 agccagcctc caggccgtcc ttgtagtcca gggtggcccc ctggaggagc tgggcgctgg
    25321 tcgggtcgac cacgacgtcg acgtcgccgt aggtgttgcg gatgtcgtcg gcggcgacat
    25381 cggtgtcgaa gtacatctcg taggagtagc cggagcaccc gccgggacgg acctcgaccc
    25441 gcagggccag gccttcctcg ccctcggcgt cgatgagctg cttcaccttg gtggcggcgt
    25501 tgtcggtgag ggtgatcaca attcctccgc agtgtctcca cgccgaacgt gggagatcag
    25561 ggacgcccac tctaccgccg gcacgccgac cgcccccagg cacggtcgat caccggtcgg
    25621 gcaccagggt cgcccgacac ggctcgcggt gcgacaggtc gtggaagtcc accacgctcg
    25681 ccccgccgat ctcaccggcg aagccctcca tcagaccgcg gtgcagggcg cacaccagct
    25741 cggggttgga ctcggccagc tcaccgaacg ggcagtgccc gaacgccacc gtggtggcat
    25801 cggcctcggt gaccgcggcg gggtcgaagc cgagctcggc cagcatcgac agcgaggcgt
    25861 ccacgcacgg ccgccgggtc ggatcgagtg cgtcgccgag cacctcgtgg tgggccaacc
    25921 gtcgcccctg ctcgcgtccc gcggcgagca ccggttcgcc gccgagtccg gcgtcgacgg
    25981 ccagctccag cagcatccga gccaacatca ggaacatcgg cggttcgagg ccgagcgagg
    26041 gcgcgtcgga ggtgagcgag tacaggtgct ggggtcggcc gacggcgccg cgactatcgg
    26101 gccgggcgtc gaccaggccg acctcgcgca tgcgctccag gtgggggcga acggtgttgg
    26161 ggtgcagccc cagcgtgtcg gcgatctcgg cggtggccag cgggcgcggt gagcgggcca
    26221 gctccaggta gatcgcgtag cgggtgttgt cccccagcgt cttgagcagg tcgagccggc
    26281 gctcggaggt ggccacggac ccaggctaac cggttgaccg agataacacg gcgcagacca
    26341 gtaaaatagg tcccgtgacc gaccagacgc cgcccgcctc gaccgacgtc ctctccgcgc
    26401 ccaccgccgg acgcgtctcc gaggaggccg tgtgggaggt actccggggc gtgatcgacc
    26461 cggagctcgg ctccgacatc gtcgagctgg ggatggccaa gggcgtgcgc atcgaacccg
    26521 acggcaccgt gcacgtgacg atcgcgctga ccaccagcgg gtgtccgctc cgggccgaga
    26581 tccaacgcaa cgtgcggacc cgcgtcggcg ccctggcggg cgtcacgcac gtgaagctcg
    26641 actggaccga gctggaccag gccggcaagg ccgcggccat ggagcgggcc cggcgcaaca
    26701 tcgcggagcg cgccgagcag accgcggtcc cgtcgtcgac gaaggtggtc atggtcgcct
    26761 cgggcaaggg cggcgtcggc aagtcatcgg tgacggtcaa cctgggcgcc gcgctcgccg
    26821 ccatgggctt caccgtcggc atcatggacg ccgacatctg gggctactcg gttccccgca
    26881 tggtcggcct cgacggacgc atgcacgccg aggagcagcc gccggagccc gacggcgcgg
    26941 cggccggcga caagcgggcc cgcatcatcc ccaacgagat cccggtcggt gacggccgac
    27001 tgaaggtcgt ctcgatgggg ttcctggtcg aggacgaggg cagcgccctg atgtggcggg
    27061 gcctcgtgct caaccgggcg gtgcagcact tcctcgagga cgtcgactgg gagggcgtcg
    27121 actacctcct gatcgacatg ccgcccggca ccggcgacgt gcagatggga ctggccaagc
    27181 tgctgccccg cgccgagatg ctgatcgtga ccaccccgtc gctgaccgcc cagaaggtgg
    27241 ccatccgcgt cgtcgacatg ggccgcaaga acttcctgca catcgcgggg gtggtcgaga
    27301 acatgtcggc ctacgtggac gacaccggac gcgagcaccg cctgttcggg accggcggag
    27361 gcgaccgtct ggccgaggac gccggggtgc cgctcctggc ccggatcccg atcgaggcgg
    27421 cggtggccca cggcggcgac cacggtcggc cggtcgcact ggccgacggg ccggcagccg
    27481 acgcgttccg ggccctggcc cgccgcatcg tcgaggacgt gatccagccc acggacatgt
    27541 cggggtgcag cagccggatg ctggacgctg tgcaggccgc tctcgacgag gccgactcgg
    27601 gacctgtggc cacccgggcc ccgagctgac gggacgagca cgacagatct gctacagcag
    27661 ggcggatgaa gatccgggtc ggattcggac tgggagtgcg cacccgcttc gtcgaccggt
    27721 cattcctcga cctcgtcgac gacctggagc ggctgcgctt cgacagcctg tggctctccg
    27781 agcgcgtgac ggccgagtgc cccgacccgg tggtcgcgct cgcagtcgcc gccggtcgca
    27841 ccacccgcct caagctcggg atgtcggtca tggtcctgcc cggtcgcaac ccggtgctcg
    27901 tggccaagga gctcgccacg ctggaccgcc tctcgggcgg gcggctactc cccgcgttcg
    27961 gtctcggggc cgtgagccgg gccgagcagc aggccttcgg cgtcgagcgg ggagaacggg
    28021 cagcgcggtt cgacgaggcc ctcggcctgg tgcgccgtct gtggaccgag gaccgcgtcg
    28081 accacgaggg tcggttcttc cgtctggaag gagccaaggt ccgaccgttc ccccaccagt
    28141 cccctcccga cgtgtggctc ggaggcgccg cgcctgccga gctgcgccgc gtcggacggc
    28201 tgggagacgg atggctgccc agcttcatca cccccgaagc cgcggccgcc gggcgggccg
    28261 tggtcgagga caccgccgcc gagcacggcc gctccatcga ccccgagcac ttcggcgtgc
    28321 tgctcccgta ctcgttcgac ccggtgcccg acgaggtgct cgagctgctg ccggcccgcg
    28381 accccgacat cgaccgtcga cgcctggtgg cggtgggcgc cgagcagctc gccgaccgca
    28441 tcggcgagtt cgtcgacgtc ggcttctcca agttcgtcgc cgtccccctc ggtgaccccg
    28501 tcgacgcgac cgatcatctg gagcgcctct cccgggacgt actgcccctc cagacctgaa
    28561 cgcccacccc caccgggcac cgccccggtc gcggggcgat cagtcgtggt cggggctcag
    28621 cgggcgggcc gtccaggcgt cgtagacgcc accgacgtgt tcgggatcgc cgggcaggac
    28681 gatgcgcatg ccgtcgtcgc cgtcgctgat cgtcggcagg atgccctcga cccgcgactg
    28741 cgcccgggcg tggtcgagca ccgcgtcggc ccggtcgaac ctcgacaggg cgacgagcgt
    28801 gcgcaccgtc gggaagatca tggtgatgtc gccggcgtcg tgacggcgga gagcctcggc
    28861 cggggtcaac cactgggtgc cgatcagctc tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28981 nnnnnnnnnn nnnccgtcgg cgtgacgggc cagcagcacc ccggcctcct cgaacgactc
    29041 ccggatggcc gccacccaga acgccagacc cccgaccgcc agaccgagac gacgcgacgc
    29101 ctcggcatcg tcgaggtcgg ggcagcgatc cgccacgtcg gcgtcggcgt cggccgggtc
    29161 gaccgcgcca ccggggaaca cgtacgcacc gccgacgaag tcggagttga ggttgcgctg
    29221 catcaggcag acctcgggtc cgtccgagcc gtcccgcagg agcatgaccg tggccgcgtc
    29281 gcgcaccggg acctgctcag gagtggggct gtccatgggc accgggctca cgtcggaggt
    29341 ccgatctccc ggggggcgtc gacgtcgacc tcgaccacct cggcgtacgc cgtcgacggg
    29401 cgccgctcgt gcgagtcggc gtcgaccacc gggccccgtc cgaagggggc gtcgatgcgg
    29461 aacgacccga agcccgcacc gccgggcccg cccggcccac cgaacgaggt cagtatcacc
    29521 gcggagcgag cggcgcgacg ctcgacccaa cgggtcatga ccgggcgcag cagcgcccgg
    29581 gtggggggga acagcagcag aagtcccggc accgcggtca ggaaccccgg gacgatgagc
    29641 aggaccccgc cgacgagcac catcagaccg tcgagcaggg tgtcggtggg cgctcgaccc
    29701 gccgccagtt cggcgttcgc ccgtcgccac gtctggggcc cctccgcacg gatcagcacc
    29761 actccgagca cgctgaggcc gaccagcagc agcagcgtgg ggaggagacc gatcgcctgg
    29821 gcgacgagga tcgcggcccc gatctcgagg atgggcaaca gcagcaggac gagcaggacc
    29881 atcaccgctc caccctacga tcgacggtcg gagcgaccca ccgcgaccgc acggttgcgc
    29941 agaggacgct cagccacacc ctccggtcgc acaccctccg gtcggtcagc cggccgcctc
    30001 gatcaggaca cggcgctccg tctcgttgag cccgccccag atgccgtggg gttcgcggat
    30061 ggccaccgcg tactccaggc actcctggca cgccggacag tcggcgcaga tcgccttggc
    30121 gcggcgttcg cgctccaggc gatcggcctt gcgctcgaac tgcggcggcg ggaagaacac
    30181 cgccgcctgc ggaccccggc aggccgcctt gacctgccac atgtcgtgga cctgttgtgc
    30241 actcaccggt cggacccccc ctttcggtgt gcggcgcacc ctacgcacgg ccgtcccggg
    30301 ggctcaagga acaatttgtg cgcgatccgc tcaccatcgc gaacatcagg gcgacggcga
    30361 gctcgggcgg caccgaccgg tggatggtcg tcggtgcccg agaaccgtca ggtcgcgact
    30421 cagtccgacg gatcgggcgc gtcggtgaga ccggtgtcgt cggcctcgac ctccagcacc
    30481 acgccgtcga cggcgacgac ccggaccgcg gcgcccgagg gcaccgacgc gtcggtgtca
    30541 gcccgcgccc gccacagccc gccggccacc ttcacgttcc cctcggggtc gatgggggtg
    30601 gcggcgcggc ccgactcccc caccagccag tcgcggtcga tggtcggggt cgagaaccgg
    30661 gtgcggacca tggccggcat gccggtgatc atcgtggcgg cgatcccgcc gatcccggcg
    30721 atgagcgcga tccacgtggg ccggaagtcg gtcatcagga acaccgacga cacggtgaag
    30781 ccgacgaggc cgatcgcggt ccacaaccgc gggatccccg tctgcacgtc gattccgaac
    30841 gcgacgaagc tcgcgacgaa cacgaccagg gcccattgcg cgtggggcag ctccgcgagc
    30901 ccgtagcccc cgagcacggt gaacagggcg ccgaccactc cggcgatccc gatgccggcg
    30961 gtgaagaact cgaacagcag gagccccaac ccgatcgtga gcaacaggta ggcgaccgac
    31021 gggctggcca ccgtgtggaa cagctgcgtg gtcagcggca gcttggagaa ccggacggtc
    31081 gtcagcggca cggttcggcg ctcgccgtcc tcggtgcggg tctcggtctt caccccgtcg
    31141 aggttgacga ggtggtcgcc gacggtgggc gagaaccgcg tgaccagccc ggcgcgcacc
    31201 gcggcctgac cggtgagggt cgcctcgaga cccttggcct tcttcccggt gaacaggtct
    31261 ccgaactcgt cgaccgagag ccgttgggac ccgacgtcac cgatcgacgc ccccggggcg
    31321 atcccggagc tgtcggccac ctgcacgagc tccgcggccc cgcccagcgc ctcggagccc
    31381 gacggcccga tccacaccga caccgggacg tcggagtcga cgatcgcccg tgccacctgg
    31441 tccagccgct catcacccac cgcggatcca ccgctgtcga actgcagcac gacgcccacg
    31501 acgccgtcgg agcgacgcgc cgaggcgagg gtgtcgacga tgaagtcggc ctcgatcccg
    31561 tcgacgagcc cgttgacggc gaccacgtcg acgcagccgt gcttcgcgca cgagttggtc
    31621 tgggcgcccg ccggcgagac ggcccacccg gcgagggtcc cgaggccgac caacaccagc
    31681 gcgaaacggg ccgcgaacct ggccgcggcg ggtcgacgga gcggcccggg acgggtcggc
    31741 gcgtccggtc ggacgatcat gctcacggca ctggctagtg tgcccaactc gagggcctcc
    31801 gaggggacgt cgtggatcct gcgcagtttc tcaccgcatc cggggtgatc atcgtcgccg
    31861 ggaagggcgg tgtgggcaaa accaccgtca ccgctgccct ggctgtcgcc gcgtcagcgg
    31921 tcgggctgcg caccctgatc gtcgaggtcg agggcaaggg cgggatgagc tcgatgttct
    31981 ccgccgacgg gttcgcctac gacgaggtgc agctgatcgc cgcggcggcc gaccacgccg
    32041 gggtgcgggc ccgcacgatc acccccgacg acgcgctcct cgactacctc ggcgaccgcg
    32101 ggctgcaacg gatctccaac cggctggtgg cgaccggact gctcgacatc gtggccaccg
    32161 cggtgccggg tatccgggac atcctcgtgc tgggcaaggt caagcagctc gagcgcagcc
    32221 gggaccagga cctgatcctc ctcgacgccc ccgcggccgg ccacgcgatc acgttcctcc
    32281 gctcggcgag cggtctggcc gacgcggtca aggtcgggcc catcaacacc caggccgagg
    32341 acgtgctcga gctcctcggc gacccgggcc ggtgtcgggt ggtgctggtc accatccccg
    32401 aggagacacc ggtcaacgag ctcgtcgaga ccgcctacag cctcgaggac accgtcggcg
    32461 tcgccctcgg cccggtcgtg gtcaacgggg tggtcccgga cctggccggt ctcgacgtcc
    32521 cgccggtcga ggcggccgcc gcggtcggga cgcgcctgct cgacggtgaa gccgaggtgc
    32581 tctccgaggc ggccgcgttc cgccgggccc ggggacgact ccaggacgac cagctgcgcc
    32641 ggatggccga cgagctgccg ctgccgcagc tgcaccttcc gttcctgttc accaccgacg
    32701 tcggacctgc tgaactcgag accctggccg acgcgctcct agaggccatc cgcgaccttc
    32761 ccgacgatcc ggcccacgac cccgacccgg cggcggtggc cggctcgccg tcgtcgggga
    32821 cgccgtgacg gcgcgcacgc tctccgaggt ggtcgaccgg gcgtcggtcg tcgtgtgctg
    32881 cggatccggc ggggtcggca agaccaccac cgccgcgctg atcgcgatgc acgccgctca
    32941 gcagggtcgc cgctcggtgg tggtgaccat cgacccggcc cgacgactcg ccgacgccct
    33001 cggtctccag ggtctcacca acgacccgag cccgatcacc ggcccgtgga acgagccggg
    33061 cggagcgggc gggtcgatgt cggcgctcat gctcgacacc aagggaacct tcgacggcct
    33121 ggtcaagcgc tactcggccg atcgcggcca ggccgagcgc atcctggcca acgcgttcta
    33181 ccgcaacatc tccggtgcgc tgtcgggcac gcaggagtac atggcatcgg agaagctcta
    33241 cgagttggcg gtcgagggcg acttcgacct ggtcgtggtc gacacacccc cgacccgcaa
    33301 cgccctggac ttcctcgacg cgccgcaacg cctcgcccac ttcctcgacc accggctcta
    33361 caaggtgctt ctgtcaccgt ccaagggcat cgggcgcgct gtcaacgtcg ccacccaggc
    33421 ggtgatgcgc cagatcgcga aggtcgtcgg ggccgaggtg atctccgacg ccctcgcgtt
    33481 cttcaaggcc ttcgagggca tggagcaggg cttcaaggag cgtgcccggg aggtcgaccg
    33541 gctgctcaag cgcccgagca ccgcgttcgt gctggtggcg tcgccccggt ccgacacggt
    33601 gaccgaggcc ggattcttcg ccgacgagct ggctcgcaag gagatcgcag tccagggcct
    33661 catcgtcaac cggacccagc cgtcgttcgg agtggcgctc ggtgacggtg ccggcgaggg
    33721 caccgatggt ccggtccggg tcgccctgag cgggtctgcg gttcgggccc gggccgagac
    33781 gctggccggc accgccctgg gcgaccacta ccgctgcctg gccgactcgc tgcacctggc
    33841 cgcgggtgag gccgagcacc tggccggtct ggccgagcgg gtcaccccgg cgccggtggt
    33901 gaaggtgccg gtgcagccct tcgaggtcag cgacctcgag tcgctgcgga cgctggccgc
    33961 cgtcgtcttc gccgacacct ccgacggtta cggtgccggc gattccgatg cgcagcaggc
    34021 ccacgactag cgtcggtgcc gtgacgacgt acctcatcgc gacggactcc gacgacctgt
    34081 tcggactcct cgacgccgcg atcggcggga ccgcgtcgct ggtccgggtg agcagcggcc
    34141 aggccgtccg accggcggtc cgggagctcg aacccgacct ggtcgtcctc gacctgcaga
    34201 tcgggaccat gggggccatg gcggtgtgca aggacctgcg cctggaggag ggcgccggcc
    34261 gccttccccg ccagaacgtg ttgatgctgc tcgaccgacc cgccgacgtc ttcctggccg
    34321 accgcagcgg cgccgacggt tgggtgacca agccgatcga tccgctgcag gtccggcggg
    34381 cagcccgggc cgtcgtggcc ggcgacggct accgacacca gccctctccc gcctgagacg
    34441 gcctccacgg catcgtcgcc gaagcgggtt tcgcgggacg cggcggcgag aactacgatg
    34501 acgtctcctt cgggtggtgg cgcagtttgg cagcgcgcct cgttcgggac gaggaggccg
    34561 ggagttcaaa tctcccccac ccgaccaacc gcacccggtc cccgcctcgc cgggggccgg
    34621 gtgtcgtgca cttcaccctg atcgggtttc tcgacgtcga agttcaaggt gggttccggc
    34681 tcgcgccgat ggatcctccg tggctgctga actcgacacc accgaaccga tcgccctcga
    34741 ccgcggacgg gtcccccgac gtcgacgggg cgtggtcgcc gtcgtggcgg tcgtcctcgc
    34801 cgtgctcatg tccggggcgc tcaccgcgtg cgagagcaac aaggccgagc tcgacgccgt
    34861 gcgcaacgcc gtcaacgcca gccgcgccca ggccggactt ccggccctgc gtgagaacgt
    34921 gacgctcgac gtcaaggccg acggctgggc gcggacgctg cgtgactcgt gtcggatctg
    34981 gcactcgagg ctgtccgacg gcgcccccaa ggagtggcgc aagctcggcg agaacgtcgg
    35041 gatgggcggc agcatcgacc agatccacgt ggcgtacatg aactcgcccg gccaccgggc
    35101 caacatcctc gatcccgcct tcaaccagat cgggaccgcg gcggtgtggg gcacctgcaa
    35161 cgggatgcgc accctgttca ccgtccacgt gttcatgaag ggctgaccgg gggttcccgc
    35221 tccgctccgc agtaccgtcg gcgccatgga gatcgtggcg ttccaacggc tgatggagga
    35281 cctctacggc gactgcgacc gccagcgcgg cgtcccgtcg acggtggcgt ggctctgcga
    35341 ggaggtcggc gagctcgccc aggcggtccg caagggcagc gtcgaggatc agctgcacga
    35401 gctcggcgac gtgctggcct gggtggcgtc gctcgccaac cagctcggcc tgtcgctcga
    35461 ggacgcggcc aaccgctacg tcaccgatcc gcccgtctga gcccggacgt ccgccagcga
    35521 tccgcccacc ggcgatccgc ccgccggcgc ggcgacggcc ctacggtcac gacgtggatc
    35581 tccacgaggt ggtggacgac gtcgtctcgg tcatcgaggc cgtcggggtg gtggtgctcg
    35641 tcgtcggcgc gtccctcgcg ttcatcacgt cgggccggga actgctgcgg cgctcgggat
    35701 cgccgggcac gggcgcctac gacgacctgc gccgcaacct cgcccgtgtg atcctgctcg
    35761 gactcgaggt gctgatcctg gccgacatca tcaagacgat cgtcgttgac cagacccctc
    35821 agagcgtggc cgtgctcggg atcatcgtgg tcgtccgcat cgtcctgtcc tggtcgctgc
    35881 aggtcgagat cgagggcacg tggccgtggg cgcggtggcg gaccggcacc gcggccgcac
    35941 cggaccgcat ggactgaccg tgatcgccat ccgaccggtc gctgacctga ccggtcgctg
    36001 atctgagcgg gccgccagcc ggtcagccct ggcgggcgat cagctcctcg gcgatctgga
    36061 tcgcgttcag cgcggcgccc ttgcgcaggt tgtcgttgga gacgaacagc gccaggccgt
    36121 gctcgacccc cggatcgcga cggatgcgcc cgacgtagga cgggtcccgg cctgccgcgt
    36181 cgaggggggt gggcacgtcg gccagggcga cgcccggagc ggtggcgagg acctcacggg
    36241 cccgttccgg cgacaggtcc cgttcgaact cggcgttgat cgacaacgag tggccggtga
    36301 acaccggcac cctcacgcag gtccccgaca ccgccaggtc gggcaggtcg aggatcttgc
    36361 ggctctcgtt gcggagcttc tgctcctcgt cggtctcctc ggagccgtcg tcgacgaccg
    36421 ttcctgccag cggcaggacg ttgaacgcga tggtgcgggc gaacttctcc ggcgccggga
    36481 aggccaccgc gccgccgtcg taggtgagct cggggccctt ggtccccacc gcctgcgcct
    36541 gacggtcgag ctcctccaca ccggccagtc cgccgcccga gaccgcctgg taggtgctga
    36601 cgaccagacg cctcagcccg gcctcgaccg ccagtggctt gagcaccggc atggccgcca
    36661 tcgtggtgca gttggggttg gcgacgatcc cccgagggat gtcgtcgagg gcgtgtgggt
    36721 tcacctcggc gaccaccaac ggcacggtcg ggtctatccg ccacgccgag gagttgtcga
    36781 tcaccgtcgc acccgcggcc gccacgcggt ccgcgtactc ccgcgacgac gtggctccgg
    36841 ccgagaacag agcgatgtcc agaccggcga agtccgccgt cgcggtgtcc tcgacctcga
    36901 cctgggtgtc cttccacggc aggagcgtcc cggcggaccg tgccgaggcg aagtagcgga
    36961 tcgaagcgac cgggaggcca cgctgcgcca ggagggtgcg catcacacct ccgacctggc
    37021 cggtggcgcc gaacacgccg atgtgacgag gtcgtgcgcc ggagaggtcg ggaccggcga
    37081 tggcgtcggg gtgggtggac atggccccca ggctacgggt cggtgtcgga tcggacccaa
    37141 cgggtatcga cgcgcgagag tcgacgtgat ggcgttcggg ctctacatcc acgtcccgtt
    37201 ctgcgcccgt cgctgcggct actgctcctt cgtcacctac gcccccggcg agctgaccgc
    37261 gtcgcagacc caccgccggt gggccgaggc ggccgtcgcc gaagtcggcg tcgcggcggc
    37321 cgggctcggc ggccacgacg actcggagcc gacggtcgtc gacaccatct acttcggggg
    37381 cggcaccccg accgcggtcg accccgccct gctcgccggc gtcctcgacg cggtgcgcga
    37441 gcggttcgca gtggccggcg acgtcgagat ctccgtggag gccaaccccg acggcctggc
    37501 gccgggaacc ctcgagtcgt tggccggtct cggagtgcgc cgtgtgtcgt tcggaatgca
    37561 gagcgtggtt ccccgggtcc tggcgctgct cgaccggacc cacgacccgg agtcggtacc
    37621 cgccgcagtg gccgcagccc gcgccgccgg aatcccgagc gtgtcgttgg acctgatcgc
    37681 cgggacgccc ggcgagaccg atgtggactg ggccaacacc gtcgccgcgg cgttggcgat
    37741 gcaccccgac cacctgtcgg cgtactcgct cggcatcgaa ggcggcaccc ggctggccgc
    37801 ccgtgtacgc cgtggcgatc tggtcgagcc gtcaccggac gaggcggctt cgcgctacga
    37861 gcacctcgac gcggcggcct cctccgctgg tctgagctgg tacgaggtgt ccaactgggc
    37921 ccggaccacg gcggatcgct gtcgccacaa cctcggctac tggcgggacg acgactggtg
    37981 gggcatcggt cccggggccc actcacacat cgacgggcgc cgttggtgga accactccgg
    38041 cctggacgac tggagcaccg aggcgcttgc gggccgggtg cccgccgccg gctccgagac
    38101 gccgaccgcg gaggagcggc tgaccgagca catcatgctc ggcatccgtc tccccgaggg
    38161 gatctccacc gatcgggtca ccgacgggtc ggtcgactcg ctcgtcgacg acggcctcgt
    38221 acgtcgcctg cccggaccga cccagcggat cgccctgacc ctcagggggc gtctgctcgc
    38281 cgacctggtc gtccgtcgac tccgttgggg ctgacccagc tcaggttccc atgacccctc
    38341 gcgttctcat gacaactgag taccgccagt gtcgtgactt gtcgtcagaa accggggtga
    38401 gggtggggga tggggtcaga aggtcgtgga ctcgagctcg taggcgtcgt ggaggacctg
    38461 caccgcccgg tccgagtcgg cctcggcgat cacgcaactg atccggatgg ccgaggtcga
    38521 gatcatctcg atgttgatcc cgttgtcggc cagcacctgg aacatggtcg cagccacgcc
    38581 cgggttggac ttcatgccgg cgccgatcac gctgaccttt gcgatcgcgc ggtcggtcac
    38641 caccgccgcc gcacccacct cggcggcgcg ctcctcgcac accgcgatcg cctcgtcgat
    38701 ctgggcgtga gggacggtga aggagatgtc ggtcacgccg ccctcggaca cgttctgcac
    38761 gatcatgtcg acgttgacgt ccgcgtcggc cagggaccgg aacagcgtgg cggccacgcc
    38821 gggtcggtcc gggaccccgg tcacggtgat cttggcctcg gagtcgtcgg cctgcacggc
    38881 tcggatgagc gcgtcctcca tgtcgggatc ctcctcgatg acccacgtgc ccggctccca
    38941 ggtgaaggcg gagcggacgt ggagcgggac cttgtggcga tgggcgtact cgaccgatcg
    39001 catggcgggc ttggggcagc ccgcggcggt catctcgagc atctcgtcga aggtgatgcg
    39061 gtgcatccgc cgcgccgtcg acacgacccg gggatcggtg gtgaacaccc ccgacacgtc
    39121 ggtgtacagc tcgcaggcgt cggcaccgag ggcgtaggcc aacgcgacgg ccgtggtgtc
    39181 ggatccgccg cgacccagga acgtcacgtc gttgtcggtc gacacgccct gggcgccggc
    39241 gacgaccggg acccgccccg ccgcgaccgc ggcacggatc cgatcggcgc gcacgtcggt
    39301 gatcttggcg ttggtgtggt tggtgtcggt cacgaacccg gcctggctgc cggtgtagct
    39361 ctcggactcg acgccccgct cggccagggc catggccacc agggcgatcg acttccgctc
    39421 gcccgcggtg atcagcatgt ccatctcgcg gccgggcgga cgcgacgaca cctcgccggc
    39481 caggcggagc agctcgtcgg tctccttgcc catggccgac acgacgagca cgacgtcgtc
    39541 gccgcggcga cgacagcggg cgacgtggtc ggcgacctcg cggatccgct cgggatcgcc
    39601 cacggaggtc ccgccgaact tctggaccag gagagccacg gcggcccacg gtactcgtcg
    39661 cgcctcgctg ctcccccaac cggatcgccc gtcgggacgc ctcggcccgg ctcaccggcc
    39721 gggcccgagc caccggtggc gacggagcgg ccgactcgga ggcggtccac cggttcgcta
    39781 ctgtcgcccg ccgtgggaac cagcgccgag aagcacgccc gtcagaagca ggcccgctcc
    39841 agcaagctgc aggaggcgac ggtccgcgct cgccagtcgt cgcgccgtcg gcggttggtc
    39901 accgtgatcg gggtcctcct gggggtcggg atcatcgtcg ccggggtgtg gatcctcacc
    39961 ggttccggag acgacgacac cgcggagggg tcgaccacca cgacgacggc gggcgactcc
    40021 accaccacga ccgcggcgga cgccgccgag ctgccccgcc cgcccgaagg gatcacgctg
    40081 accgagaaga ccccgtgtcc tccggccgcg ggaaccgaga agcgcgtgga acgcttcgcc
    40141 ggcccaccac cgacctgcat cgaccccgat gccagctaca ccgccacgtt ctcgaccacc
    40201 aagggcgact tcaccgccac gttggactcg aagaaggccc cggtgtcggt caacaacttc
    40261 gtggtgctgt cccgctacca cttctacgac ggcgtcccgt tccaccgcat cgtgcccggc
    40321 ttcgtcatcc aggccggtga cggcgacggc gacccgtggg gcaacaacga cctgggctac
    40381 agcatccacg acgagctgcc ggcctcgtcc gccgagtacg tggactactc gctcgccatg
    40441 gccaactcgg gtccgaacac caacggcagc cagttcttcg tggtgctgcc cggcggcggg
    40501 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcaggccg gttcggggcg
    40621 cagcagcgac ggcaggtctg ccagatcggc gggatggccg tcgcggtaca cggtgacgtt
    40681 gccggccccc tcgacccgcc aggtgccgtc gggggagtgg atgagcgcgg tcgcctcgtc
    40741 gatggccacc acggccaggc ccggtcgggc gagctcgacg gtgcggtgca gcttctcggg
    40801 cgaccagccg ttgctgcggg ggatgacggt catggtctcg atcaggtcga gcccgacggt
    40861 gtaggccccg ccgcggctgt cgaccatgtg gctcgacagc accgacgccg cctcgccgga
    40921 cgcggcgacg gtcgctccgg cgcgccacgc gccgaccagg gcgtcgaaca gcggggtgtc
    40981 cttgagcacc gagcggaggt gcatgggcga gccgccgacg acgtagacca tctgcgcgtc
    41041 ggcggccctc tcggccgggc ccggctcgag cgcctcggcg cgtcggtaca cgtcgagcac
    41101 cgacaccgcc acgccccagc ggccgaacca cgcccgggcc cggtcgaccg cggcggcggg
    41161 gttctcatag gccagggctg acggcaggac cagcacctcg gtcacccccg ccaccagcgc
    41221 ctcgtcgaag gtgcagccct cggtccactc gcggccgccc accaacgcca aggtcccggt
    41281 catgaccacg acggtacccg ggaccgggca cggcttgggc acgcccggtg accgctcggt
    41341 agcatcgccc gccatgacga tcgaacgtgt ggggatcctg ggctcgggca tcatggggtc
    41401 gggcatcgcc gaagtggcgg ccaagtccgg cttcgaggtg gtgctccgct cccgcaagca
    41461 ggagtcggcc gacgccatgg tggcggccct cgagaagtcg ctggcccgcc aggtcgagcg
    41521 cggcaagctc gaacaggcca ccgcagacga ggtccgggca cgggtcaccg ccacggacca
    41581 cctcggcgcc ctggtcgact gcgacctggt catcgaatcg gtcgtcgagg acctggcggt
    41641 caagaagccg ctgttcgcgg agctcgactc ggtcgtcaag gactcggcca tcctggccac
    41701 caacacctcc accctgccgg tggtcgacat ggcggtcgcc accgagcgcc cggaccgtgt
    41761 ctgcggcgtg cacttcttca acccggcgcc ggccatggcc ctggtcgaga tcgtccgccc
    41821 gctcaccgcg tcggacgaga cgatcgcggc ggtgaccgag ttcgcgtcgg cctgcggcaa
    41881 gaacccggtg caggtcaagg accaggccgg cttcatcgtc aacgccctcc tcttcccgta
    41941 cctcaacaac gccgtgcggc tgctcgagaa cggcaccgcc accgccgagg acatcgacac
    42001 cgcgatgaag ggcggctgca acttcccgat ggggccgctc gccctgctgg acctggtcgg
    42061 cctcgacacc tcgctgtcga tcctcgacgc cctctacgac gagttccgcg acccgaacta
    42121 cgccgcggtc ccggtgctgc ggcggatggt gtcggccggt cagctcggac ggaagtccgg
    42181 cgccgggttc tacgactaca agaagggctg atccgggatc ggcgctccgc cccgggccga
    42241 gccacctccc agccggtggg tgttcccgcc gctgcacgac gcggaccccg acgggcccgt
    42301 cgtgctcggc ggtgacctgg aaccgggcac cctgctggcc gcgtaccgtt ccggtctgtt
    42361 ccccatgccg atcggccggc gccgactcgg ctggtggtca ccggaccccc ggggcgtcat
    42421 ccccgtcggg ccccgagcgg tcgccgcggg caccgcgctg cacgtcagcc ggtcgctgcg
    42481 gcgctcgctc cgacgcttca ccgcgacggt cgacgccgac ttcgaggcgg tcatgcgcgc
    42541 ctgcggagac cctcgccgtc cgcacggctg gatcgacgag aacttcgtgg gcgcgtacac
    42601 cgagctccac cgccggggct gggcgcactc ggtcgaggtg tgggaggacg accagctcgt
    42661 cggcggggtc tacggcgtgt cgatcggggc gttcttcgcc ggcgagtcga tgttccaccg
    42721 ggccaccgac gcgtccaagg ccgccctggt gcacctgacc gaccggttcg cccacgtcga
    42781 ggacgccctg ttcgacgtgc agtggctgac gccgcacctg gcgtcgttgg gcgccgtcga
    42841 gatcccccgg tcggagtacc tcacccggtt ggcgtcggcg gtggcccggc ccgtcgaccc
    42901 gttcggctga ccgcgggtcc gctcaggccg acggtgccgc cgccaccgtg ctgcccccca
    42961 gacgcttgtt ctgctccacc cgccagatca ggaacgtgac gatgccgatc accacctggg
    43021 ggatgaacgt ggcggcccgc cacagcaggt tggcggccag cgcctggtcc ttgtccatgc
    43081 cgaacgccga catcagtccg accagcgcgg cgtcgacggt gccgagcccc ccgggcgtga
    43141 tggggatgaa gccggccagc cgggcgagag agaacgccgc gaacaactcg agcaggttga
    43201 tgccggtgct cttgccgccc aacccgtaca ccgccacggc caggacggcg aactgcgaca
    43261 gctgctggcc gaagttggtg acggtcagcc ggatccaccg gtcgtggacg acgtcgacgg
    43321 tctggtcccg gaatcgcaac gcccccgccg tcagcggacc gtcctcgccc gcagcgggag
    43381 cggttcgcgg ccggagccgg gcgagcagcg cgtcgcccca gccgcccagt cggcgggccc
    43441 agccctcgct gcgcaggatc aacgccaggc ccccgaccat cacgccgagg gccaccagtg
    43501 acaggaccgc tcccaggagc gcgccgctgg tcgcctcgtc ggacagcacc agggcgagca
    43561 ggccgagcgc gggaagcgcc agggtgacca tcaggttcca cacgctggtc acgccgatgg
    43621 ccgaggtcga caccgccggg tcgatcccgt agccgttgag catggcgtac tggacgccca
    43681 gcccgaacgc gccgccggcc gggacggcgt tggagatcat gaacgacgtc tggcgcacga
    43741 tgaaggcccg cccgtaggcc aggccgggaa gggcggcctg gtaggggaag acgtagatga
    43801 ggatgttcgc gacggtcgcc agggcgaaca gggccagcga gctggccgac atctcctgga
    43861 tcgactccca cgcctcgccg tagttggcga acttggggaa gatcccgaag aacacgatga
    43921 ccagcaccac cagggtgacc agtcccgcga tgacctgctt gcgccgcgac ggccgggtcg
    43981 gccccgccga cgccgactgg tcggcggcgg gcccacccgc cggttcggtg ccgacgggtg
    44041 agggtggatc ggtcacgtcg gcccacgcta tcggcagaca ccgcccgtga cgcggctcgg
    44101 cgtcgccgac ggacccggcc gctgaggcac agcgacggcg tcgtcaggcg ttcttcggca
    44161 actccgagaa cggcagggtc ttgtcgttgt tgggctcctt gggcagcccg agcacccgct
    44221 cgccgatgat gttgcgctgg atctggtcgg tcccgccgta gatcgacggc ccctgcgccc
    44281 acagcgatac ccaggtgacc aggttgagga acgggttgcc ggtggcgtcc tcgatcgcgg
    44341 ccttgtcggc gtcctcgtag gcgtggagcg tcccggccgg accgacgatg cgcaggccga
    44401 ggtcgcggat caggcgcatc atgtcgctca tcgccagctt ggagatgttg ggcatgccgg
    44461 ggatgtcccc gccggcctgc ttggccgcct tgagccgcag gttgttgtac cggccgagct
    44521 cgccgagggt gtggagccgg accaggtcct ggcggacggt cgggtcgtcg agagcgccgt
    44581 tgctgcgggc caggtcctgg agcatcttgg gcatggagcc gagcatgccg atggccccgc
    44641 ccccgccctt gcgggccccg ctcttcttgg gggcgaagtc cccggcgcgg gccccgagct
    44701 ggtcggcgac gctgcccggc gacaccgagg tggccgcgcc cgacccgcca ccggagccga
    44761 gtccggcccg ctcgtgcatg agggtcgtgt tggtgacggc ccacccgttg ttgcggtcgc
    44821 cgatgatcgc atcggctcgg acgtgggcgt cggtcaggaa cacctcgttg aacatggcgt
    44881 ggccggtcat ctccttcagg ccccgcacct cgacgcccgg ctggtgcatg tcgaaggcga
    44941 accaggtgat cccctggtgc ttggggacgt cggggtcggt gcgggcgatc agcatgccca
    45001 tgtcggcggt gtgaccgagc gaggtccaga ccttctggcc gttcacgacg tactcgtcgc
    45061 cgtcctcgac ggcccgcgtg gtgagcccgg ccaggtccga accggcaccg ggttcgctga
    45121 acagctggca ccaggcgtcg acgccggtga cgatgcgccg gacgtagtgg tcgatctggg
    45181 cctgggtgcc gtgggtggcg atggtcggcg cggcgagcag cagtcccagt ccgccgggcg
    45241 cccccagtgc gccgtgctcg gcgatcgcgg cctgtgcccg cacgctgtcg ttgcggctga
    45301 gccccttgcc gtaggcgtgg gtcggaagtc ccggcgccgc ccaacccgac agcccgagcc
    45361 gctcccacca ctcgccgacg gtgagctccg ggtcccagtt ctgatccagc caggcctgga
    45421 gctcctcgag gatctcgtcg gagctgccgg cgtcggactg atcggtcgag gtgtcggtca
    45481 tggtcgtgct cccggaagtc gcggtcggcc cggcccgcgc cggacgtctt gtcccggggt
    45541 tccccctccc acccccggcg accgacagta tgtcacggtc gcgaccctgt tgttgaccga
    45601 ccggtcaagt tgcggttgcg agggtgggta gggtgctcgg ccatggagac cagagccgcc
    45661 atcctgtggg agcccaacac gccgtggagc gtcgagacga tcaccctcga cccgccccgc
    45721 gccggcgagg tcctggtcga gatggtcgcg tcggggatgt gccactccga cgaccacgcc
    45781 cgcaccggcg acctgccggc gatcaccccg ctgatcggcg gccacgaggg ttctggagtg
    45841 gtgctcgagg tggggccggg cgtgacccgg gtcgcccccg gcgaccacgt cgtgttcagc
    45901 ttcgtgcccg cctgcgggca ctgcccgtcg tgcgcggacg ggcactccaa cctgtgcgac
    45961 aacggtgcgg ccctgctcgt cggcgcccag ctcgacggca cgacccggca ccactccgcg
    46021 gacggcaccg acctgtggac catggtgtgc ctgggcacct tcggcgagca cacggtggtc
    46081 aacgagatgt cctgcgtgaa gatcgacgac gacatccccc tcgagctcgc ctgcctggtc
    46141 gggtgcggcg tgaccaccgg ctgggggtcg tcggtgtacg cggctgaggt cggcccgggc
    46201 gagaacgtcg cggtgatcgg cgtgggcggc atcggcatgg ccgcggtgca gggcgcccgt
    46261 ctggccggcg cccgtcagat cgtcgccgtc gacccggtcg agttcaagcg ggagcaggcg
    46321 ctgaagatgg gggccaccca caccgcctcc agcatcggag aggcgttcga gctgatccag
    46381 gagatcacct ggggtcggat gtgcgacaag gtcatctgcg cgatgggcgt cggcgagggt
    46441 cagcagatcg caccgatcat gacgctgtgc gccaagcgcg gtcgggtcgt ggtcaccaac
    46501 atccaccccg ccgaggagac gagcatcgac atctcgctgt gcgacctgac gctgatggag
    46561 aagcagctgg tcgggaccat cttcggatcc gccaacccca actccgacat cccccggctg
    46621 atgcacctgt accgaaatgg ccagctggac ctggccggga tggtgacccg cgagtacgcg
    46681 ctcgacgaca tcaaccaggg cttcgacgac atgctcggcg gccgcaacat ccgcggggtg
    46741 ctccgctacc ggtgagggat acccccaggg gtacttgacg tcatacccct cggggtacca
    46801 tcatggacgg ccatgaccgt cacggagcac atcgccgaca cactcgtccg gggctcgggc
    46861 gacggaccgc cagccggtcg accgccgttg ttcggtcgac tcgcccggtt ctcggtccgt
    46921 cggcgccgct gggtgatcgc cgtgtggctc gtggtcgtgc tcgcggcggc tccgctcgcc
    46981 ctcacgctca ccagcggcct gtccggcgcc ggctgggaag cccagggctc ggtggcgaac
    47041 caggtgcgcg acgagctgag ggccgacttc cccgagctcg gcgccgagtc cgcgatcgtc
    47101 gtgtaccgcc aggagcagcc gatcgtgtcg gacccgtcag ggttgatcgc ggtcgtcgac
    47161 cagctccggg gcgcccccgg cacggcctcg gtgcaggacc ctctccagct gcctcccgag
    47221 gcggggatga tctcccccga cggcaccacg gcgatggtcc cggtccacct caccggcgac
    47281 gaggatgcgg atctgcccga gagcgcggcc cgcgtgatcg accacgtggc cggcatcgag
    47341 gtcccggacg gcgcccgtat cgaggtgacc ggcgagtggc ccgtgtggtc ggacttcaac
    47401 gcctcgaacg aggaggccct ccacaaggcc gagctgctgt ccgggctgcc gacgatcatc
    47461 ctgctgctcg tggccttcgg ctccgcggtc gccgccgggc tcccgctcat cctggccgtg
    47521 gccggcatcg cggtgggctt cgcgtcgctg cgcctgttcg gcatggtcac gccgctgtcg
    47581 gtgtggtcca tgaacttctc gatgatgatc ggcctcgccg tcggcatcga ctacagcctc
    47641 ttcatcgtct cccgctaccg ggaggagcgt gaggacggcc acgacgccga gcacgccatg
    47701 gccaacacgc tgacgacggc gggcaaggcg gtgttcctgt cggcgctcac cgtcgtcctg
    47761 tcgctcgccg cggtgttcct cgtcccggtg atggtgttcc gttcgatggc gctcgggatg
    47821 atcctggccg tcatcgcggt cgcgctcgcc tcgctgaccc tgcttcccgc cgtgctcgcc
    47881 gccatggggg accgggtgct ggtgtcccgg ggccacgacg atcccgaccg cgccgcggag
    47941 ggtcgttgga cccgctggac ccagggggcg ctgcgtcgac cccggatcac ccttgcggcg
    48001 ggactcgtgc tcctcctcgc cctcgccgcg cccgccatcg ggatgcgcct cggcatgccc
    48061 ggcgcccggg tggtcgacga gggtcgctcc agccgtgacg gctacgagat ggtcgtctcc
    48121 gcgttcggcc cgggagctgc cgctccggtg ttcatcaccg tggccgccgc cgactcccag
    48181 gcggtgctcg acgtcgccgc ggccgacccc ggtgccgcgg cggcatcgat cgtcgcccga
    48241 cccgccgagt ccggtcggac cgtggtgcgg gtgatcccga ccaccgcggt cgactccgct
    48301 gagacgaccg aactggtgtc gcggttgcga gccgacgtgg ccgaggtcgc ccccggtgcc
    48361 ctcgtcggcg ggccggccgc ccagaaccac gacctgaccg acgtcctcac gggccgggcg
    48421 ccgctcgcca tcggcgtcat catggtggtg gcgttcctgc tcctcctcgt cgtgttccgc
    48481 agcctgacga tcgcgctgtt ctcgatcgtg ctgaacctga tcagcgtggc cgctgcgttc
    48541 gggttcgcga ccatcgtttt ccagcacggg atcggcgccg gactcatcgg catcgagcac
    48601 cagggcttcg tcgacgcctg ggccccgctg ttcttcttcg ctctgctgtt cggtctctcg
    48661 atggactacc agctgttcct gctcgccgcg atccgtgagc gctacgagca caccggcgac
    48721 accgcgactg cggtccgcga gggcatcgcc cggaccgggc ggccgatcac caacgccgcc
    48781 ctcatcatga tcgtggtctt catcgccttc ggcgtcaccg gaccgatccc gcccaccgag
    48841 ctgggcgtca ccctggccct ggccgtcctg ctcgatgcca ccatcgtccg gatgatgctg
    48901 gtgccgtcga tcatgggcct gctcggcgag cgcaactggt ggctcccccg ctggatgggg
    48961 aagcggctgc ccatcgtccg cttcgaccac tgacggcacg acgctccctc ggctgttcgg
    49021 gctcggggct caacgtgcca gatcgaagcc gatcggccac tcgagccgat gacggcccag
    49081 cagctcccgg tcctcgagca gcgaccgggt cggggcgtcg gccacgatgc ggccccggtc
    49141 gaggatgacg gcgcgggagc acgtcgccag cgcgaacggg aggtcgtggg tgacgatcag
    49201 ctgcgtcgag ccaagcgtga cgagcagctc ggcgagctcg cgtcgcccca ccgggtcgag
    49261 ccccgaggtc ggctcgtcca ggaccagcag gtcgggctgc atcgacagca cggtcgccag
    49321 tgccgcccgt cgggcctcgc ctccgctcag ctgatacggg ttccggtccg cgagcgcggc
    49381 ggcgccgacc gcggccaggg catcgtccac ccgctggtcg cgttcggcgc cccgtaggcc
    49441 caggttggac ggcccgaacg ccacgtcgtc acgcacggtc tgcaggaaca gctggtcgtc
    49501 ggggtcctgg aacaccatcc ccacccgacg gcggatctcg cggaggttcc cgtcctgcac
    49561 cggcagtccg gcgacgatca ccgatccgac ctgacgttcg atcaggccgt tgaggtgcag
    49621 gacgaaggtc gtcttgcccg agccgttggg gccgagcagg gctacccgct caccgctctc
    49681 catccgcagc gacaggtcct cgaggacgac ccccgaagct ccggggtagc ggaacgccag
    49741 gccctccacc tcgagtgccg gagcgtcgga ccgcgtcacc gccccacaac ccagacgacc
    49801 accgcgaccg ccgacaccgc ggccggggcg agcgcagcga cccagtccgc cgtcgcgacc
    49861 gctggtctgt cggacaccac cagggtcccg gtgaagccgc gcgccaccat ggcgccatgc
    49921 acccgttccc cgcgctcgta ggagcggacg aacagcatgc cgaccgacgc ggcgacgggc
    49981 ccgacctgcc agagccagcg ggggtcgtgt ccccgggaca ccatcgcggt gcgcatgcgg
    50041 gcgagctgcg cggcgaggag ctcgacgtag cggaccatcg acgacacgat ggccacgacg
    50101 acccggggga cgtgcaggcg agccatgccc tcgagcatcg acggcaacgg cgtcgtcgcc
    50161 gacatgacga tcgccgcggt tgcgcccagg gtcgtcttgg ccaccacgtt ccacgcggcc
    50221 cacaaccctt cggtcgacac cggcaccccg cccacctcgg tgcgcggccc gtgcgcgacg
    50281 aacgggatcg ccagagcgaa cacgatgaac ggcgtgacga cggccaggcg agcgagcacc
    50341 cggcgcgctc cgatccgcga gaccgccacg acgacgacga gcacggcggc ctcgagcgcg
    50401 aagaccgcga cggcatggcg gggggttccg gcgacgacca ccgcgaaggc gaccgtgccc
    50461 acgagctcgg ccgcggggtc cagccggtgg accggcgagt cgaccgcgac gtggagccga
    50521 cgcaggttgc cgcggtgtga gcccgacacc gttcgcgctc cgccctgcct gatcagctcg
    50581 acgacgtcgt gtggagcgac cagcggggcg ggacccgccg ggtggcggtg gtgaggccgt
    50641 aggcgaccag cagggtcacc acgaccccgg ccagacctgc gaccgccagg ctcagctgag
    50701 cgttgccgat gccgcgggtg gcgtagtcgg cgaaggggct gcccgcgacc gcgtggtcct
    50761 gggctcggtc gatgaagccg tggtcctgag ccacccgttc gaggccgtcg gggtcgtcga
    50821 aggcgaactg gctgaccacc acggcgaaca cgattgccac cagcactccg ccgatggcga
    50881 aggcgcgggc gccgaccggg cgccggtcgc cgatctcctc ggcactgagg tcccgggcgc
    50941 cggcgacgag gtcgggtcgg gcggccatca ccgcaccgac gaccagggcg ctgaggagac
    51001 cctcgccgat tccgatgagg gcatgcactc cgaccatggc cccgaagacg gtgtcgaacg
    51061 ggatgggggc ggtcgccccg aagagccact cgatcgagaa ggccgtggcc gacaggacga
    51121 ccgtcatccc gcaggccaga ccggccgccc cgaccacacc gccggaggac cggggcatca
    51181 ccttgcgcca cagcaggaac agcgcccatc cggcgaaggc cggcacgatc gccatgttga
    51241 ggacgttgta gccgagcgcg gtgatcccgc cgtccgcgaa cagcagtgcc tggaccacca
    51301 cgacgaccgc caccaccagg gcaccgaccc acggcccgag gaggatcgcg gcgagggcgc
    51361 cgccgagcag gtgacctgtc gtccccgccg cgaccgggaa gttgaacatc tgcgcagcga
    51421 agatgaacgc ggccgagata ccggccagcg gcacctgacg gtcatcgagc tcggccgacg
    51481 catggcgcag cgcgaggccg atgaccacga tgctgatgac ggcggtgacg accgcgaccc
    51541 cgggaccgag gaatccgtcg ggggcgtgca tggcgaccag catcgacgac gacgctactg
    51601 caactccatc gcagtagctg ccgcctggtt cccggtccgg gccgcgggcc ccggctcacc
    51661 gatcggtgca gtccgagcag cgacccgaca gggggaagtg caccaggtcc aattcgatgc
    51721 cggtggcgtc gcgcacccgc gccgcgaagg acctcacctc cgactcgggg agctccacgg
    51781 ttcgtccgca cacccgacac ccgaccggga ccgtctctct gccccgtcgc cggtagaccg
    51841 ccccgccgtg gccgacatgc acgtgctcgg cgatgcccag ctcctcgaac tgccccagcg
    51901 tccggtagac gaccgactgc tcgagacccg accgttcgac gatgtcgtcg acgctcagcg
    51961 ggtccgtggc gtcgaacagc aggatggcca ccgtccgctt ggcccgggtg acccgaccgc
    52021 cgtggtcgcg gatgcgctgc aacgcctggt cgacgtcggc gggcggtgcg ggtggactga
    52081 ccgcggcggt ggtcaccacc gaagttgagc acacgtccca caccgcgacc gggcgccgtc
    52141 cggtggatgc gacgagaccg aagcgatcgt cagtcggtgc gggccatgtt ggcgaacaac
    52201 gtgcagtagt cgaggaacac caaccgggcg atgccggtgc gcccggcgcg gtgcttggcc
    52261 acgatgagct cggccatgtc cttgtcctgg gtgtcggggt ggtacacctc gtcgcggtac
    52321 aggaacatga cgacgtccgc gtcctgctcg atcgagttgt gggcgatcac cccgtcggcc
    52381 acgaagttgt gggtgccgag cacggtggcg tcgaacaccg gctggtcccc gatcgaggtc
    52441 accgacacga cctcgtccca catcacgtcg gagtcggcga gcgcggcgag ctccttgctg
    52501 tcgacggcgt ccgcgatgcg ggccaggcgg gtccgactgg tggcgcgtgg acgcgactcg
    52561 gtgcccagca ggtacccgcc gcagtaggac tcgccgagcg cggcggccag gtcgcgctgg
    52621 ctcatctcca tgcgagccag ctcggagacg accagatcac gcacctcgca gggcaccgtg
    52681 tccacgttcg ggttggcggt gatctggtcg aggacccgca gggcctggtc ctggtcccgg
    52741 ccccggtcgc cggggacgac gatctggctg aggaacgccc tctgctggtc gacgccgtgg
    52801 atccacaccc gccaccgggg tcggcgggtg ccgacatcgg tgatgcgggt ctgcaccccg
    52861 aagcgcaaca gcagtcgctg cacgtcgtcg atcagccggc gcgaggtgct ggtcgccatc
    52921 agacgcacca gcggcttgcc ccgcagcgaa ccgacaccga ggaacccgat ccgggcgaac
    52981 agctcgccga ggaacgcggc caggaccgcg tcggaggcgg cgtcgacctc ggccggcacg
    53041 gcccggtccg caaccgcacg ttcagcgagc gccccggcgt ccgccgcggt ggccgttccg
    53101 acccgttcgg gcgcaccgaa gcgtcgcgga acagctatcc gcgctccgac ggcgagctcg
    53161 tccaggcgtc gccaaccggt gacggtccgg aacgggtggt tggccgtggc ctcgaccgtg
    53221 cgacccgacg ccaactccat gcggaacgcg gacttggtgc cgctggggaa ggcgtgggtc
    53281 aacgtcgcgg ggaccatctt ccagtcgtgg tccaggctcc acaccggcac gtcgcgctcg
    53341 ccggtgctga ccagctcgcc cagggtgacc tcctggttgg tgtcggcgcg cagcagacgg
    53401 gtgcccgcgg tgaggcaccc cgactctcgc aggtcggcga gcatgggtcg cttgtcggcg
    53461 cgcagctcca gctggcgcga cagctgcgac agggccatca cggggcagcc gagctcgcgg
    53521 gccagcacct tgagccctcg gctgatctcg gccacctcga cctgacggct ctccgccgag
    53581 gaccgccccg acatcagctg caggtagtcg acgacgatca gccccaggtt ggtgcccaac
    53641 cggtcctgga ggcgacgcgc cttggcacgg atctccatca ccgtcagcgc cgggttgtcg
    53701 tcgatccaca gcgagccctc gcccagtcgg cccatcgcct tggtgatctt ggtccagtcg
    53761 gcgtcggtga gccgtccggt ccggagcttg cccgagtcca cccgggcctc ggccgcgatc
    53821 agacgctggg tcagctccag gtgtcccatc tcgagcgaga agaacagcac cgggaggttc
    53881 tcccggaccg ccgcgtgcgc ggcgatcccc agggcgagcg cggtcttgcc catcgccggt
    53941 cgggcaccga ccacgatcaa cgctccgggt tggaggccgg cgagcatggt gtcgaggtcg
    54001 gtgtagcccg acggggtgcc ggtgatggcg tcgccgcgct cgtagagcat ctcgagctgc
    54061 tcgaggctcc ggtcgagcag cggcgacagc tcctgcaggg tgtcggcgtt gcgacgctgg
    54121 gcgaccgcga acatcatcga ctcggcggtg tcgacggtct tctctatgtc gtcgagcggc
    54181 tggtagccca gctctgcgac ctcgttggcg gtggagatca gccgccgcag cagcgccttc
    54241 tcctcgacga tgcgggcgta gtgctcggcg ttggtgatcg ccggggtccg ggcctggagg
    54301 gtgaccagcg tggccggacc gcccacggcg tcgagcacgc cggccgaggt gagctcctcg
    54361 gcgacggtga ccgggtccac gccctgaccg gcaccgtaga gggactggat cgccgcgaag
    54421 acgtggcggt gcgccggctt gtagaagtcg tcctcggtga cgatgttcgc caccgacgag
    54481 atggcgtgct cggacagcag catggcgccg aggagcgact gctcggcgtc gatgctgtgg
    54541 ggagggatcc gcgaacggat gaccggctcg tcgggggcgc gggacaggtc ggacatgcgg
    54601 atctcatcct cggtccggtg ccctgtgggc atccagacca caaccccggt agttcagggg
    54661 atctccctgt ggagatcccg tggatgagcg ggcggtcatc caccgccgtc cacagacgca
    54721 ccggtcggcg cctccgacgg tcctgaccgg gttccggtca cgtggtcgat cctccatcgg
    54781 ggcgaccgta gcgagggggt gcgacacccc cgccgtgccg gtcgttcgcg gcgcccgtgc
    54841 ccggacgcga tcgaggccgg acccgacggt ccggcctcga tcgtcgacgc tgggctccga
    54901 ccgtgcggcg cggagctctc cggctcagcc ctcgaccacc tcgatggtga tcgggaactg
    54961 cacgtcggtg tggagacggg cgaacacgtg gtgcgtgccc agctccttga tgtgctcgtc
    55021 catgtggagg tccttgcggt cgagctcgat gccggtctgc tcggcaacgg cgtcggccac
    55081 ctcggtggtg gtcaccgacc cgaacagccg accacccgaa cccacgcggg ccgggatctc
    55141 gatcgtccgg gcgaccagca ccttggcgat ctcctcggcg ctctcgcggt ccttggcgtc
    55201 cttgaggtca cgggagcgac gcatggcctc ggcctgcgcg gtggcgccgt cggtggccgg
    55261 gaacgcgagc gaccgagggg ccaggaagtt gcgggcgtag cccttggaca cctcgacgat
    55321 gtcgccgcgc ttgcccagcc ccgacacgtc ggagcgaagg atcaccttga cggcggtcat
    55381 gcctcgctcc cctcggtcac ggcctcggtc tcctcaccgg cgttctcgat cccctcgaac
    55441 tcggaggtgg ccggagccgc ctcgtcgcgc tcgggacgct cggagcgatc ggggcgatcg
    55501 ccgcggggac ggcggtcgtc gccgtcgcgg tcgcgacggc cgccacgctg ggtggtgacc
    55561 cggttggtgt agggcaggag cgccatctcg cgggcgttct tgatcgcctt ggcgatctcg
    55621 cgctgctgct tggtgtcgtt gccggtcacc cgccgggcac ggatcttggc ccgatccgac
    55681 atgaagcgac gcagcaggtt ggcgtccttc cagtcgacgt agaccacctg ctcgctgttg
    55741 aggatggaca ccttcttctt gacgcggcgg cccgtgtcct tgggcttgcc ccgcttctgc
    55801 tgtggcttgg ccatcagaac ggctcctcgt tgtcgtcata gccaccggag gggggtgcgg
    55861 tgggtggggg ggcggaatcg aagccgccgc cctcgcggcg ctcggtcttc tggacctggg
    55921 cggtggccca gcggaggctg ggaccgacct cgtcggcgag gacctccacc ttggagcgct
    55981 tctcgccgtt gtcggtctcc cagttgcgct gctgcaaccg gcccgagacc atcacgcggg
    56041 tgcccttggt gagcgactcg ctcacgttct cggccaggtc gcgccagcag acgatgtcga
    56101 agaaggcgac ctcctcctcc cactcgttgc tctgctggtt gcgccagcgg cggttcaccg
    56161 ccaggccgaa gttcgcgacc gcggccccgc tggggatgaa ccgcagctcg gggtcacggg
    56221 tcaggttgcc gatcagctcg acgtagttgc ctgccatggt cgtccttccg ttcagccggc
    56281 cgcgctcagc aggccgcgac gagcggcctc ctggtcgggc agccggatca gcttgtggcg
    56341 gacgacgtcg tccgcgagtc ggaggaagcg gtcgatctcg tcgaggttgg gtgcctcggt
    56401 gacgatctcg aggacggtgt aggtgccctc gttcttcttg ttgatcgggt aggcgaaccg
    56461 gcgccgaccc cagttgtcga cggtgggggt ggtcccgccg tccgcggcca ccatctcctt
    56521 gacccgctcg atgaccgccc ggttggcggc gtcgtcggcg tcctggtcga cgatgatcat
    56581 cagctcgtac gcgcgtgtcg gcacgttgca gttacctccc tctggacctg gacccatcgg
    56641 ccccagggtc ctgcgatctg caggacaggg cgcccggacg accgggctct tggaatgacc
    56701 ggacgagtgt agtggtccac tccccctcat ccgccagccg gccgccggtg ggtcgacgac
    56761 aacgctcgcg ctgccctgtg acactcgggc gtgtgacgtt cgggcgatcg gccggcggcg
    56821 cgcggacgag ccggccccac gacgccgggt cgggcccgac gcctgtccgg ctcagtcgac
    56881 cgccgcgccg tacagcgaca gggcctctgc ctgggcggag tcgaggtggt agctctcgga
    56941 gtggtccggg aacgccccgc cacgcacatc agcgacgaac tcgccgaccg cggcgaccga
    57001 cgcctgcttc agatcggcgt agcgacgcac gaacttggcc ggggtgcggt cctcgagtcc
    57061 gaggaggtcg tggtacacga gcacctgtcc gtcgcagcgg gggccggccc cgatgccgat
    57121 ggtggggacg tcgacggcgt cggtgaccat gcgggccacc tcgtcgggca cgccctccaa
    57181 cacgatggcg aagcagccgg cgtgcgccag ggccttcgcg tccgcgacca gcgcgagggc
    57241 cgcctcggac tccttggcct ggacccggaa gccgcccatc gcgtgcatcg actgcggggt
    57301 cagacccagg tggcccatca ccgggatctc ggcgtcgatc aacgcctcga tcatcgtcag
    57361 gcgcttggag ccgccctcga gcttgacgca ctgggcgccg gctcggatca gctgcgcggc
    57421 gttgcggacc gtctcctcga ccgacacgtg gtagctcatc cacggcaggt cgcccaccac
    57481 cagggccgac ggtcgggccc gggccaccgc cgcggtgtgg tgcgccatgt cctcgacggt
    57541 caccgacaac gtgtcgtcgt agccgagcac caccatggcc aacgagtcgc caaccaggat
    57601 catgtcgacc ccggcgtcat cggcgatgcg ggcccccggc gcgtcgtacg ccgtcaccat
    57661 caccagcggg tcggcaccgt cagcgacctt gcgggaacgg acccacggga ccgtgatccg
    57721 cgccggacgc gacggggccg gtgccgcagc cggggcgcgg gcgcccgccg cttcgctggt
    57781 cgccgccgac gcggtcgagt cgtgagcaga gtcgtgagcc atggcccacc tcctg
//
LOCUS       DAOQOD010000004        44589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_250_length_44589_cov_11.599542, whole genome shotgun sequence.
ACCESSION   DAOQOD010000004 DAOQOD010000000
VERSION     DAOQOD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 44589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44589
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_44589_cov_11.599542"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="murF"
                     /locus_tag="PLZ72_01110"
     CDS             <1..1093
                     /gene="murF"
                     /locus_tag="PLZ72_01110"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNA34922.1"
                     /translation="ARAGLDGTGPAGVGLAGTGLARVVGVTGSVGKTTTKDLLANVLA
                     RRFATVASEKSFNNELGVPLTLANAPAGTEAAVIEMGARGAGHIRFLCEMARPTIGVV
                     TVVEGVHTEVMGPIDQIARAKRELVEHLPADGLAVLNAADPNVAAMAAHTVAPVLSFG
                     TGGEVHAEAIELDDELRARFTLRSSWGPTEVHLGVRGAHNVTNALAAAAVGLWLGVDP
                     REVAAGLAEAPASPWRMELLRVPGGPTVLNDAYNAGPASMAAALAAVAALPAPGAGRR
                     IAVLGLMAELGDEGPAEHRRIAELADELGVEVLAVGCGLYGSPWTVDSADDVVGALGK
                     LGPDDVVLIKGSRVAGLERAAEALTRPGR"
     gene            complement(1029..2321)
                     /locus_tag="PLZ72_01115"
     CDS             complement(1029..2321)
                     /locus_tag="PLZ72_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA34923.1"
                     /translation="MEVLRGSKDFSGGGRRSDRTPGTLSSAYSQPAFRRIWYGNLASN
                     IGTWMQNITLGVLAYQLTGEAWFIGVITFAQLGPMLLLSPIGGALADRVDRRRLMIAI
                     AISQTVLSMVLAVVALSDQPNRIALVLIVFGIGVGASINGPTFSAMLPELVGRDDLQG
                     AVALNSAAMNTSRVVGPILGGFTAAIGGPALVFAANGLTYLFVVWAVATVHADFSPKG
                     HSGTSPWRQIHEGVAAARADGVVTRILITISTFSLCSLVFIYQMPLIAEERLGIDGIA
                     YTLLFASFALGAALGAISMGTVFSELPRSRMSTGSIWVFAAALAVFGLTTSTWVAFPA
                     VFVTGGAYFILVTALSTTLQLRVDDSVRGRVMGLWMMGWAGLVPVGGLIAGPVIDSIG
                     EVPVLLFGALVAAALGLLIDLDESAPPPPVPGLPLGSP"
     gene            2466..3218
                     /locus_tag="PLZ72_01120"
     CDS             2466..3218
                     /locus_tag="PLZ72_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNA34924.1"
                     /translation="MAERTHYQVLGVRQSAPLAEIRGSYRQLAYRLHPDRQAGVTEAE
                     RQLAERRMREVNAAWTTLSDPAKRAAYDRTLVPRGSGASGSGSGTRAGAGSTAGAGAS
                     GRPGASDAGSGQWWDADDPDAVLARAKAAEAAEVDALDEEPDLSGVQFWLLRRGVVVA
                     MVIVGLIIFVASAYAGRGVTDPTATSAPPAMVADSECVKVMADHMAYNVSCEGSYDAR
                     IVREEPNARSCLDDGLEYAVVRARSVCVDEDV"
     gene            complement(3261..4601)
                     /locus_tag="PLZ72_01125"
     CDS             complement(3261..4601)
                     /locus_tag="PLZ72_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HNA34925.1"
                     /translation="MLESDLRDIVDRARHLATGAAGPDQISDEGLEDLAVTLESARRA
                     LDAATARINGEIDARKSTDRRHGHSTKTWTAGTLRLPSEEAGRRVKVARLLRSCPDLH
                     EALRLGRIGFDHVRVIANATNARNVDAVIGIQQQLIDMTDDFLLFRAWAAQVLDLLRL
                     ADPDGAEPSVEDNHLNLSVQFDGSARLEATLVGDSRHVVEHALSVFSDRVFAQLSSDA
                     AQAPEDLPLPHHSAIRALALGEICRVALAATHKGPGTAADVTFVIHAEDPGVVRTHDG
                     ERVDSYTAQVAVCDAAFHPVVFDRFGVAVDLGREQRFGSRDQRRASRVRDGGCVFPGC
                     DAPSSRTDLHHVDPWVPTGSSSTGHHSDVCSDSCHHTGADDTSTRGTSTDSTATGGRT
                     DMDNLASLCRHHHGVVHRTGWAMRTVEHQWFEFTTPTGAVLGSQRHGRPMPAAA"
     gene            4931..5007
                     /locus_tag="PLZ72_01130"
     tRNA            4931..5007
                     /locus_tag="PLZ72_01130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4965..4967,aa:Val,seq:cac)
     gene            complement(5126..5518)
                     /locus_tag="PLZ72_01135"
     CDS             complement(5126..5518)
                     /locus_tag="PLZ72_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA34926.1"
                     /translation="MSAFVDTNILVRHLTGDPADMAARATAYLAEAEELYLTDLIVAE
                     TVYVLESFYEAPRDQVATAMRSLVSMRSVVTVDPALLLRAIEVYETDRLDFAETYLVA
                     CAESTGVGSVASFDRSIDRVDTVDRMEP"
     gene            complement(5515..5763)
                     /locus_tag="PLZ72_01140"
     CDS             complement(5515..5763)
                     /locus_tag="PLZ72_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNA34927.1"
                     /translation="MEIAARMSSKGQVTVPKAVRDALGLGEGDDVVFRVEGDRAVLAR
                     TPEFLSLAGTIAVPAPKRNVAWDEVIRSTRAKRAATAR"
     gene            5965..6183
                     /locus_tag="PLZ72_01145"
     CDS             5965..6183
                     /locus_tag="PLZ72_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HNA34928.1"
                     /translation="MARRSSIPTAERVRAYRERLRAQGLRPVQIWVPDVRSPQFVEQA
                     HLQSAAVAASTHESDDQAFIDALGSDDG"
     gene            6180..6512
                     /locus_tag="PLZ72_01150"
     CDS             6180..6512
                     /locus_tag="PLZ72_01150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNA34929.1"
                     /translation="MRRGEIWTAAAGSGYVGKPRPVVIIQDDRFDATASVTVCALTTD
                     PTEAPLFRLPVTPDETNGIREPSNLMVDKITTVPRAKLGEKIGRLRDDDMVRLGRAVI
                     VFLGVAGS"
     gene            complement(6603..7265)
                     /locus_tag="PLZ72_01155"
     CDS             complement(6603..7265)
                     /locus_tag="PLZ72_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA34930.1"
                     /translation="MTIRVLIADDQRIVRAGLRLILDAEPDIEVVAEAVDGREAVELA
                     EDLRPDVCLLDIRMPELDGIEATRLLAGPDVTDPLAVVVITTFDLDEYVHGALKAGAR
                     GFLLKDAGPDLLAQAVRAAANGDALIAPSVTARLLSTFSGLGDSTREPAAQPIAPLTE
                     REEEVLVTVALGRTNAEIADALCISLSTVKTHLASLMNKLGARNRVELAMWAYETDRV
                     PT"
     gene            complement(7262..8410)
                     /locus_tag="PLZ72_01160"
     CDS             complement(7262..8410)
                     /locus_tag="PLZ72_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNA34931.1"
                     /translation="MALETSRRPWAQPRVEDPPGPARRDWLFVLVVVVAATVETFTDD
                     QLVWPAVSLVTTAGLAFLLPWRRVRPLLVVAVSFGTVSAIEVASILGDVEWDGLNAAA
                     FMLVLPYALARWASGRDVGIGLGVVAVPIALSVVAGDQFGYVLGGTLVVLLAGAIGVA
                     VRYSVELRVEEVEARRSRERAELARELHDTVAHHVSAIAVQAQAGRVVAATSANGALE
                     ALEVIEEEASRALEELRSMVGALRDDRTDLRPQQGIGDLSRLERHGGLSGPRVTVRIA
                     GHLDGVRSSVDAACFRLAQEAVTNALRHARHASEVEVRVDGDDDNIHLTVVDDGRGVV
                     ASTATATGFGLIGMAERARLLGGTFEAGPRPDGGWAVTATLPRQAVTT"
     gene            8655..9374
                     /locus_tag="PLZ72_01165"
     CDS             8655..9374
                     /locus_tag="PLZ72_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA34932.1"
                     /translation="MIEVRGLTKHYGSRRAVEDLSFDLEAGRVTGFVGPNGAGKSTTM
                     RMMVDLARPDRGEVRYDGLRYADLAQPARTVGAVLDARCMHPGRTARNHLRAIAALSA
                     IPGSRVDEVLADVGLERVAGERAGRFSLGMRQRLALATALLGDPRVLLLDEPSNGLDP
                     DGIRWLRNRLRRFADNGGTVLVSSHLIAELARFADDLVVVGGGRLIAAETVESVTSRT
                     GMSLEDSLLEMTATSAEFASA"
     gene            9431..10261
                     /locus_tag="PLZ72_01170"
     CDS             9431..10261
                     /locus_tag="PLZ72_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNA34933.1"
                     /translation="MTTIDAPLRPTPEAPGAADAPNATGPRPARRFAAVLRSEWIKCH
                     TVWATKALLAVAAVAGLVISWATAAFSTGPVLTAAEVFVYPTLLTAVLAAVAGILLFT
                     SEVQHGTLAVTLAAHPSRWGVVAAKTVLAAAIGVVLGAVGMSAGFLGSRIGGLDVGDT
                     SGLAAKVMWALLYTVGAGLFGLGVGMVVRHSAGAVSGLLVWWLVVEGLVVSFAPAGLV
                     RFVPFDAGARTLDIATELDRPEVVAAALPNPLHALIFWSYVAAAVGLGAVLLRRRDVG
                     "
     gene            10326..10793
                     /locus_tag="PLZ72_01175"
     CDS             10326..10793
                     /locus_tag="PLZ72_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNA34934.1"
                     /translation="MHRNVETVIAAAGEKGLAIEPRPFPEGTKTAQDAAAAIGVEVGQ
                     IVKSLVFLVDGAPVMALVPGDRRLDESLLAEAVGGSVVERPDADAVRDAVGFPIGGVP
                     PIGHALPVYIDRGLLAYDEVWAAAGTWTDVFAVAPDDLLRITSATLCPLSETE"
     gene            10915..11421
                     /locus_tag="PLZ72_01180"
     CDS             10915..11421
                     /locus_tag="PLZ72_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34935.1"
                     /translation="MILRRAVPDDATAVAEMHVRSWQVGYRGLVPDEDLDTLRPESWA
                     DRYTFGSDAASDPMTIVALRDDAVVGFVTTAPTFDDDVDGSGQLCALYVDPAAWGSGI
                     GSTLLRAGRKNLIYRGFTDAVLWVLVGNRRAERLYERDRWRPDGARRTEEIRGIEVDE
                     RRFRRRLV"
     gene            11463..11822
                     /locus_tag="PLZ72_01185"
     CDS             11463..11822
                     /locus_tag="PLZ72_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA34936.1"
                     /translation="MEPIGVHHVAVNVTDVEAAVEFYTAVLGGRVRDDRPDLGIGGAW
                     IDLGEAQVHLVELPVPPSMGQHFAVRVDDLDAAVVELRAGGYDVADPSQVGAGLQTFL
                     TDPSGNTVELHQVLEGL"
     gene            complement(11945..13069)
                     /locus_tag="PLZ72_01190"
     CDS             complement(11945..13069)
                     /locus_tag="PLZ72_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA34937.1"
                     /translation="MDSSAGTESVEQFRARAGAWLAEHRGDAPRDYGAILPPGLAAEG
                     RRWQKTLFDAGFAGITWPVEHGGQGLSPEHGGAWITECALAQVPPFLNMVGCVLTGGA
                     VMAFGTPEQQAEHLRPILTGERIWCQLFSEPDAGSDLASLSTRAVRDGDEWVVDGQKV
                     WCSNGRVADRGILMARTDPDARPHKGISFFLIDMASPGVELRPLRQMNGDAEFDEVFL
                     SEVRLPADALLGPQNEGWMVGMSALTNERGYIGASGISLKRRLDSMLALGGDLDALDQ
                     QELAELWIRGTALWAMGRRQGPVASVLGSVAKLGTTELMFDTALLRSSLAGPEAMLDG
                     DAAYGLTSAPGARIAGGTSQIQRNIIGERILGLPKEPKPG"
     gene            13154..14542
                     /locus_tag="PLZ72_01195"
     CDS             13154..14542
                     /locus_tag="PLZ72_01195"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA34938.1"
                     /translation="MTTVGATSSPYVSEAPRLGFVGGFDGVRGIGILMVLSNHAYSAL
                     SPSFAGIIDVFFVMSAFLITTLLLQEHRDQNTISMRKFYSRRAIRLLPSAYLCIAAWL
                     VVTILFARDRLTTLLAESAAAVTYLYEIFFPVGLGQLDPKLVEHLSIDQFWSLAVEEQ
                     FYLLIAVTVLVCIRKRWMVQLAVGLAAVATFIGVQRYMGIAGPVPIPSDPAHANPVVR
                     GLSLLWLSRPDSLMWGVGLAVLNSKLPEPLPTVWRRWLPRVGLVGLVVSGVSMLLASG
                     FLFDLANKVGVPYPFVPIGPLSMEQAGDRIYWINFGHTLTALAFAPAMLAMARVKEWW
                     PNRALSWKPLRFLGRMSYTVYVWHTLVYFIVLDLLGGNERLGEKWRVPILAAIAIVAC
                     LPVYYGVEQRMLRVKLRYAAEKEVLDLNTGKMIPVEQARVMHRGMVSAEAVGDGLDGG
                     PADIAPHDEGTR"
     gene            14539..15912
                     /locus_tag="PLZ72_01200"
     CDS             14539..15912
                     /locus_tag="PLZ72_01200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA34939.1"
                     /translation="MTTTTTARGPFVSSAPKLGLVGGFDGIRGIGVMMVLVGHALFVY
                     FESLVTIVDTFFVLSGFLITTLLLQEHRNTGDIGLRKFYARRAVRLLPSVWLFCAVWI
                     VIGVIGTVLHIEAISLKGILEDSAAAVAYVYHLFFPNGLYMIEPDVQNNRYMWHLWTL
                     SVEEWFYFAIPTTVLVCIRRNWIKQLGWLLGAGIVAIGVARWFAYTGFWQDDAGMISG
                     IRLALLQRPDGLMLGALLAIVNAHLTAERIDRLRKPIVVVATVGLVVWAVALTTSFGL
                     FEKLGLPYFKYLPDGPEQFSRPQMLAQSPVYWFRWGHTLGIFAFGAILFGLVHCRRWW
                     LARAWSWRPFQWMGRMSYTLYVWHALPYVFIIALTGGEEASPTVQVLRLPVLIAAAFA
                     VSLPVYYFVELRALRVKLRFSAEKEALDLTTGKMVRVEDGKVSSLPPESDPGGAADRG
                     PDGPPSA"
     gene            complement(16000..16893)
                     /locus_tag="PLZ72_01205"
     CDS             complement(16000..16893)
                     /locus_tag="PLZ72_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA34940.1"
                     /translation="MSDLTFDGKVAIITGAGGGLGKSHALELARRGARIVVNDLGGSV
                     SGEGDNASAAQLVVDEIVAAGGEAVANHDSVATPEGGKAIVDAAVEAFGTVDIVINNA
                     GILRDKTFHNMTPELLEPVIAVHLLGAFYVTQPAWVIMREKGYGRVVNTSSNSGLLGN
                     FGQANYGAAKLGLVGFTRVLANEGRSKGIKVNAIAPVAKTRMTEDLLGPLGDKLEASE
                     VTPTVVYLASEECPVNGEVYSVAGGVVARYFIGLTPGWYQEGHSVEDVRDNFEQIRDE
                     TGYIVPEDPSGELRKLLETLS"
     gene            complement(17123..18751)
                     /locus_tag="PLZ72_01210"
     CDS             complement(17123..18751)
                     /locus_tag="PLZ72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA34941.1"
                     /translation="MAGWNFAELWEAIADKFPDAQAQVQGEKRFTWREFDSRSDALAH
                     HLIEGGAVHQDKVALYLYNCPEYMEGCYAAFKAGMVPVNTNYRYLDDELVYLWDNADA
                     VAVVFHGTFTERVATVRPQLPKVRQWIWVDDGTDARPDWADDYAEVVATSAGRAIPPW
                     GRSGDDVVMIYTGGTTGMPKGVMWRQDDLIGVTCATGNALLAADPADVGGVDAFIDSI
                     AAPGSAGLPACPLMHGTGWFTANMFLTQAGSVVTLVDRSLDWTEVLDTIERERIGALT
                     IVGDAFGKPMVRALDARPGHWDLSSLMLVASSGVMWSEPVKEAMLAHNPGMFLVDTFS
                     SSEAIGMGSSISAGENAEKTAHFALGPGCQVFGDDGKPVEAGSGERGRVAVPGRVPIA
                     YYKDPEKSAATFIEFEGVRYSMPGDYATVDADGTISLLGRGSVCINTGGEKVFPEEVE
                     EALKTDPSVADAVVVGLPDDKFGEAITAVVEPAAGSPIDESALIAHVKGHLAAYKAPK
                     RVVSIDTIGRAPNGKVDYKRMKAYAADELGIPLD"
     gene            18851..20014
                     /locus_tag="PLZ72_01215"
     CDS             18851..20014
                     /locus_tag="PLZ72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA34942.1"
                     /translation="MDFELPGDDDPRRAAVRAWLEANPDPSGRQLAEAGYVAPHWPAP
                     WGLDADPIHQIIIDEELRRAGVRRPNNPIGIGWAGPTILEAGTDAHKESYLFGLLSGE
                     DTWCQLFSEPGAGSDLAALSTRAVRDGDEWIVNGQKIWTSLAHHSRYGILIARTDPDA
                     PKHQGISYFICPMDLPGIEIRPIVEMTGAHNFNEVFLTDVRIPHDLLVGEVNQGWRLA
                     KVTLANERVSLSSGGALWGMGPDAQDLLEAIRSAGGLTDPIERQRAAELHIESEILRL
                     IRLRTVTAAIKGRQPGPEASIRKILADEHGQRIMGLAKDLAGPAGMLPSGGPLGTDVN
                     LWHYGYLFAQALTIGGGTGDVQRNIVAERVLGLPHDVDVEQGRTWAESRRSGG"
     gene            20047..20880
                     /locus_tag="PLZ72_01220"
     CDS             20047..20880
                     /locus_tag="PLZ72_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNA34943.1"
                     /translation="MVGPRVFVTGGAGTVAGWLVRTAPAAIDLHVTEHRTLVPDGVRT
                     RASVHRLDLRRRDEVERRISEIAPDVVVHTAYVQDEPAAIVDATAAVAAAAASVGASM
                     LQLSTDVVFAGDRPPYDESSEPDPVSDYGRWKAEAERRAAAILADVCITRTSLVVSID
                     PPDRATAGLVEALRACRPITLFHDEMRQPIRAEDLAAELWALVALDRADRAGVWHLPG
                     PEHLSRSDVGLRLARRLGLDPSPVAVGTAVDHPSPRPRDPALVSRRRQVLGVRLQAVD
                     A"
     gene            20900..21130
                     /locus_tag="PLZ72_01225"
     CDS             20900..21130
                     /locus_tag="PLZ72_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34944.1"
                     /translation="MTSSTDNDELKVNAKVVAKDDLPGVPAGTPGRVTLKNGFRWTRY
                     RVEFDNGVGIGSLDRAQLVPRKEYEAQQQAAG"
     gene            21190..22239
                     /locus_tag="PLZ72_01230"
     CDS             21190..22239
                     /locus_tag="PLZ72_01230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA34945.1"
                     /translation="MKLSMQIGYSGGFAESVAQVQALEKAGMDIVYVAEAYGYDAPTL
                     MGYLAAETETIQIASGILPIYTRTPTLLAMTAAGLDELSGGRAVLGLGASGPQVIEGF
                     HGVPYDAPIGRTREIIDICRKVWKREEPVVHDGKRYQLPLPEGQGTGLGKPLKMITHP
                     RRADIPIHVASLGPKNVQMTAELADGWLPILFHPERAKDVWGADVDAGLAKRSAELGP
                     LDVIAGGLLSIGPESQVGGFRDFTRGMVALYVGGMGARDRNFYNQLFQRYGYEAEAKQ
                     IQDLYLDGKKQEAAAAVPESFLEETSLCGDEGYVKDRIAQFKAAGVTVLNVTPIAPDL
                     EGQQRMIAKVKELSA"
     gene            complement(22385..23077)
                     /locus_tag="PLZ72_01235"
     CDS             complement(22385..23077)
                     /locus_tag="PLZ72_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34946.1"
                     /translation="MESKGTHTHERSRRARSVRRGVAAVGVATAGLLAWTLPSQAQDP
                     EPAPTTNFHMAGIADPAAGTMLAVDKQISGEFQYCSNANGLGAELYLNAGDGAGMAVE
                     QATVTPFVVPIHIGVVSINNPDPENTITGERFLTLGVVKGTPIEFGQSGPATVEYGGV
                     FIGPKAGLINWTYSATASCTVSRAMPIISDLMDLGTLPAPATTTTTEAPTTTEAPTTT
                     EAPTTTEAPPAP"
     gene            complement(23298..24254)
                     /locus_tag="PLZ72_01240"
     CDS             complement(23298..24254)
                     /locus_tag="PLZ72_01240"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNA34947.1"
                     /translation="MQTRLTEMLGIEHPVMLAGMGGVSYAPLAAAVSNAGGIGTLGAS
                     TMGTDEMAAQIAAVAAATDRPFGVDLLTAAPQDMTAKVHAIAAGGGSLFVAGLGVPGE
                     VIDLCHELGIVVASMCGKVAHAVRAADAGCDLVIAQGTEAGGHTGQIATMALVPQVVD
                     AVGDRVPVVAAGGIFDGRGLAAALTLGAVGVWVGTRFIATPEAQAVAGYKERILASAE
                     DGTIITRAYTGKTCRVLRTGYARAFEESGGVPEPFPMQYVRSVEDGANHLGGDLDTPG
                     VDPDREFMPAGQAIGAIDELVPAGDIVRSMVAEAEAVLAGLS"
     gene            complement(24285..25517)
                     /locus_tag="PLZ72_01245"
     CDS             complement(24285..25517)
                     /locus_tag="PLZ72_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNA34948.1"
                     /translation="MEPAEEQIDVAVIGAGPAGTAAAITLARAGIDVTVFDKATFPRD
                     KTCGDGLTTLALRQLEQLGLDPADVASWIDVSDCFVTSPSGVSIHLPLPPASDGRFAA
                     VTTRLDLDAALVEVARKEGATVVEGAGLTAAREESNAVVLRIGADGGDEREVRARWVV
                     AADGMWSPTRRALGLAEPGYRGEWHAFRQYVGNVGERAATELHVMFEADIVPGYFWSF
                     PLPGRRANIGFGIQRGGKVPVGDMGPLWEDLLARPHIRDLLGPDVTPEAPHRAWPIPA
                     RIDTAVTSTPRVLFTGDAVAACDVMTGEGIGQALLTGVRAAESIRDRAAVGDVATHYH
                     RTVTEELVADHRMSQLLVRALQHRKGARAAIRVAGATPWTRRNFARWLFEDSPRGIAF
                     TPSRWHRGALSGPGAYRS"
     gene            complement(25579..26577)
                     /locus_tag="PLZ72_01250"
     CDS             complement(25579..26577)
                     /locus_tag="PLZ72_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HNA34949.1"
                     /translation="MQHRYLGRSGLKVSAIAYGNWITHGSQVDDDTATAAVHEALDQG
                     ITTFDTADVYAATRAEAVMGAALADQRRESLEICTKVYWPTGSGPNDRGLSRKHIQES
                     CEASLRRLRTDHIDLYQAHRYDHETPLEETMVAFADLVRSGKVLYIGVSEWDAEQITA
                     AAELAAELRIPFISNQPQYSLLWRVIESEVVPASAAVGVGQIVWSPIAQGVLTGKYRP
                     GEPVPEGSRATDDHGGAGFVQRLLRDEVLEAVQAMKPLVDAAGITMAQYAVAWVLRNP
                     DVGAAIVGASRPEQLTENVVAAEITLDDDLVAAVDAVLEPVIERDPRKTVSPPTRP"
     gene            complement(26621..28030)
                     /locus_tag="PLZ72_01255"
     CDS             complement(26621..28030)
                     /locus_tag="PLZ72_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA34950.1"
                     /translation="MDDDPSATPIHYHHRMSDEDALMWSIEKDPMLRSTITAVVLLDR
                     PVDHGELRRSFDRLSRTIPRLRQRVRSNPMSVAPPRWEVDPAFDLDYHFRLARVPGDG
                     SMRDLLRMVQPIAMQGFDRARPQWECTAVDGLADGRGALIMKLHHAMTDGVGGVRLML
                     ELFDLEPDAPERDMPPPPPVHVLNQVERFNDAVDHQVRSSLGLLRELGAAGVHVAASA
                     ASDPVGTFANGAELAASAARVLRPSPDPLSALMSGRSLSNHLEVLTLPLDVTRKAAKR
                     VGGSINDAYVTGLVRGLQHYHQRHDTPLHDLRMGMPVNVRAGEMTDTAGNAFVPARFE
                     IPVHAQDIFELMRTIRRRLLSARDEPANQLVEPMANLLNRLPTTVVTQVFGSMMKGLD
                     FQASNVPGSPLPLYLRGVRVDSIVPFGPLAGAGCNVTLLSYENDLNIGLNLDPAAVED
                     PDSFIECIRLGYDEVLDLA"
     gene            complement(28117..29745)
                     /locus_tag="PLZ72_01260"
     CDS             complement(28117..29745)
                     /locus_tag="PLZ72_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNA34951.1"
                     /translation="MSTPRKALVALVAAPLALILVLVSAYAVDAAVLTSDSVARNVEV
                     AGVSVGGLSRKQLGAAVDEMAAEFPATKVSIDAGDLHLESTAGDLGMSIDTDGTVARA
                     WAVGRDDPLPTRPVRWAGSVLSPRAVDAELTLDGAALSRTLVELQGDKRSDPVEPSIT
                     ITDGAIAVVPGKDGIDLTTNSVAAALPSGVAEVGRPIRIEVKRAVTEPKMTDEAVQAL
                     AVRANQATTGKVTLTSGGRTFEIEGKRFRPAFTVTAAGTPEHPTPQLGMDAARVAELL
                     ATNMPPGSRNPTGVRFDIQGGVPVPVPGKDAQVCCGPDAPQKLADALLAGKTEVDLPT
                     RTVTAAEGAEWARTLGVTQVVGEFTTRHPAGQPRVRNIHTISDATRGILIAPGDTFSV
                     NDTIGKRTAEKGYVPAPVIENGEHTEDFGGGISQYATTLFNAAFFAGLDIPAYKAHSE
                     YISRYPFGREATLAYPSVDLKIHNNTPYGVVIWPTYTNSSVTVQLWSTPYARGEQTAQ
                     NPTGGCGKVTTERTRTFVDGHTDKQNFHASYRCI"
     gene            29916..30833
                     /locus_tag="PLZ72_01265"
     CDS             29916..30833
                     /locus_tag="PLZ72_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA34952.1"
                     /translation="MGICEGRVCVVTGAGRGIGREYALSLAAGGARVVVNDLGGEMDG
                     GGNPTSGPASEVVAEIVAAGGEAVANADDVSSFDGAARLIATAVDAFGDLHCLVNNAG
                     ILRDRMLVNMTEAEWDDVIRVHLKGTFAPTHHAANLWRTQAKAAEAGERDRPVARVIN
                     VTSVSGIYGNVGQTNYGAAKAGIAGFTFIASLELARLGATVNAIAPVALTRMTEGLGP
                     EPTEEQRELMSPRWIAPVVTWLASEESSHVTGRVFEASGRMLAVAEGWHRGPSADPVE
                     DPARVGPIVEDLLARARPPADMNGADRTT"
     gene            30956..31816
                     /locus_tag="PLZ72_01270"
     CDS             30956..31816
                     /locus_tag="PLZ72_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNA34953.1"
                     /translation="MPINPDAVGTRSDPTESSWRSKDGLLYAVGVGAGQDPLSETERP
                     FFSENSIDTTQRILPTMVVTVPDVSGAFRAIGSFNPAMLVHGEERIVLHREVPVEGTV
                     STVAEITAVWDKGKGAVVEITSTSTLVGETEPLFEVVMSAFIRGEGGWGGERGPSGPR
                     HQAPDRDPDAVVTYPTRPEQALVYRLSGDRNPLHSDPGFAAMAGFDRPILHGLCTYGF
                     TGRALLHELCGSDPARFRSMEGRFSSPVMPGEDLTVQMWRTGDGAAVYRTLASGDRVV
                     LDSGAVTFDA"
     gene            complement(31965..32624)
                     /gene="nucS"
                     /locus_tag="PLZ72_01275"
     CDS             complement(31965..32624)
                     /gene="nucS"
                     /locus_tag="PLZ72_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNA34954.1"
                     /translation="MRLVIARCSVDYDGRLTAHLPEALRLIMVKADGCVAIHADGGAY
                     KPLNWMNAPNVVVEAEDHWTVTNPKGEQLTIHLHDVVSDTAHELGIDPGLRKDGVEAH
                     LQELLAADPTVIAADLTLVRREFPTDIGPVDLLCRDADGTAVAVEVKRRGEIDGVEQL
                     VRYLERMDRDPTLRGVRGVFVAQVVKPQAKVLAEARGIDWVEVDYDVLRGAREADLTL
                     F"
     gene            complement(32784..33311)
                     /locus_tag="PLZ72_01280"
     CDS             complement(32784..33311)
                     /locus_tag="PLZ72_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNA34955.1"
                     /translation="MAATTTDLTRRTSGIFTFPNPVNEVAARTVAAGVVLMAVTFVVT
                     GWGWLLVPLTYGFIARVLTGPTLSPLGRIATQVVAPRLAEHERLVPGPPKRFAQGIGV
                     VFTVGASALWLAGASGAARVVIAMLAGAAFLEAAFGFCLGCRIFAGLIKAGVVPDSVC
                     EECNDLSLRIPALRR"
     gene            33490..34452
                     /locus_tag="PLZ72_01285"
     CDS             33490..34452
                     /locus_tag="PLZ72_01285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA34956.1"
                     /translation="MPSPTPSDPNVPQLSMMLRSFSAEDPGGWEPMLEHAVAMDRAGV
                     DRLAVSDHVCFGEALDAYGDPAVGGTAGGRQPTGPDGHWLEPLTVLTAVAARTSRIRL
                     STGILLAALRRPVVLAKTAATIDVLSGGRLDLGVGVGWQREEYRAAGLPFERRGRLLD
                     HTLEVCRTLWTEPRADHDSEHLHFEGIHQMPKPRQPAGVPIWVSGTVNPAAMARLATF
                     GVGWIPWGDEVSDLPGAIVRMREGVSAADRDPSEIGVQGRVPVRRRDDGSVDLDATMK
                     AVPALVEAGVTDVRLDIAVPDGADPVEHLGEVVERFRAVTGRSD"
     gene            complement(34495..36369)
                     /gene="typA"
                     /locus_tag="PLZ72_01290"
     CDS             complement(34495..36369)
                     /gene="typA"
                     /locus_tag="PLZ72_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNA34957.1"
                     /translation="MTDPSSLPVPGIRNVAIIAHVDHGKTTLVDAMLQQAGAFREGAE
                     TVDRVMDSMDLEREKGITILAKNTAVRWTGEGRDEVKINIVDTPGHADFGGEVERALT
                     MVDGVVLLVDASEGPLPQTRFVLRKALTLDLPVILAVNKVDRADARIDEVVAEVEELF
                     LDLDADVHQLDFPIVYCNARAGRASITKPDTVDDLAEDLTPLFELLVDHLPAPTHDPA
                     APLQAWVTNLDASQFVGRLAMCRIVNGTIRKGQPVAWCRADGTVERAKVAVLYVTENH
                     ERVEAASAGPGEIIAVAGLEDVTIGETLADPDDPRPLPVITVDEPSLAMTIGVNTSPM
                     AGLDGDKMTARLLKGRLDSELVGNVSLRVLPTERPDAWEVHARGELQLAVLVETMRRE
                     GFELTVGKPQVVTREIDGTLHEPVEAVTVDVPEEFLGAVTQLLATRKGQMTDIVNHGT
                     GWVRMEQRVPARGLLGFRTEFLTETRGTGMIHQVFDGWAPWFGEIRSRDRGSVVADRR
                     GPVTAYAIIAIQERATLFVAPGDDVYEGMVVGENSRSEDMDVNICREKKQTNMRAAGS
                     DNTERLTPPRVLSLEQALEFVAEDECVEVTPEVIRLRKVQLDAGDRARATKALKHARA
                     "
     gene            complement(36456..38042)
                     /locus_tag="PLZ72_01295"
     CDS             complement(36456..38042)
                     /locus_tag="PLZ72_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNA34958.1"
                     /translation="MTDAAVSPDTERFTFTENGPWVLDRDSLAWARGLPVLRMTVRRQ
                     VPILTGRRRLPPPLRLTEVSVRLGAAVGGWALRDRRAGGPTSRAGLSRRLRLAAERLG
                     PTYIKLGQIISSGEGLFPPELVEEFRKCRDQVRAEPFDVVRRVVEEDLGRSLEDVFTS
                     FDREPLAAASIAQVHVARLRTGEEVVVKVQRPTIRQQVQSDLKVMSWLAPFLVGRIPI
                     AALANPPALVELFAETILEELDFRLEAENMLDVAEVFAQLGQRGWIIARPHPELVTRR
                     VLVMERLEGFAFEDLESMQAAGIDTHEIIRIGMKGFTEGCIVHGIFHGDLHAGNLFVT
                     PDGRIALLDFGITARMTPLQRSAFLRMMLYGAMGDIPGQVAAFRDLGALPADTDIDEV
                     IRELGLDKAPVDPTTLDQEQLVGEIQRIIKALMGMGARLPKILMLYVKNLVFLDGAIA
                     VLAPDLDLVAEVASLSTGLATTHGERFANELGVSADGFAVDEAAIRASFGIVDDSTEK
                     VTYSELRRRRETIRRRLDRR"
     gene            complement(38071..38823)
                     /locus_tag="PLZ72_01300"
     CDS             complement(38071..38823)
                     /locus_tag="PLZ72_01300"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNA34959.1"
                     /translation="MATEDDHRLAHDLAREAGDLLLSMQRSATAADARELGARGDAAA
                     HRLLVERLTVLRPEDAVLSEESTRSEREDPRRLGASRTWIIDPLDGTREYSEGRSDWA
                     VHVALVEDHEPTAGAVALPARGMVLATDDPPSLPAAHQPPRVVVSRSRPPIEAGQMAE
                     ALGAELLPMGSAGAKAMAVVRGEAELYPHSGGQHEWDSCAPVAVASAAGLHCSRLDGS
                     ALRYNQPSTYLPDLLICRVELAEAALSAAGRQ"
     gene            38952..39860
                     /gene="cysD"
                     /locus_tag="PLZ72_01305"
     CDS             38952..39860
                     /gene="cysD"
                     /locus_tag="PLZ72_01305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNA34960.1"
                     /translation="MITYELTNLQALEAESIHIMREVAAELERPVLLFSGGKDSIVML
                     RLAEKAFWPARIPFPMMHIDTGHNFDEVYEFRDRRVAELGVKLVVASVQESIDQGRAV
                     EQTGPRASRNQLQTVTLLDAIELHGFDAAFGGARRDEERARAKERVFSFRDEFGQWDP
                     KNQRPELWNLYNGRHHRGEHIRVFPLSNWTELDIWQYIEAEGIEVPSIYFAHEREVFQ
                     RDGMWLAVTPFVTLMDDEVPETRTVRYRTVGDASCTGAVESTASTVAQVIDEVASTRI
                     TERGATRADDRASEAAMEDRKREGYF"
     gene            39860..41113
                     /gene="cysN"
                     /locus_tag="PLZ72_01310"
     CDS             39860..41113
                     /gene="cysN"
                     /locus_tag="PLZ72_01310"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNA34961.1"
                     /translation="MELLRFATAGSVDDGKSTLIGRLLHDAKSIFEDQLDAVTDASQR
                     MGLDAPNLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHIQYTRNMVTGA
                     STADLAVVLVDARKGLLEQSRRHAFIASLLRIPHLVVAVNKMDLVDYSQARFDEIVEE
                     FRQFAMKLEVADLAFIPISALHGDNVVQRSLNMDWYEGASLLHHLESVHIASDRNLID
                     ARFPVQYVIRPQSDEFHDYRGYAGQVAGGVFKPGDEVVVLPSGLTSHIAAIETYDGPV
                     DEAFAPMSVTIRLSDDIDVSRGDMICRVNNRPIVAQDVDAMVCWLSDAAELTPRTKLA
                     IKHTTRSARALVNDLQYRLDINTLHRDESAERLALNEIGRVTMRVTQPLFIDEYRRNR
                     VTGSFILMDEATDTTVGAGMLLAGS"
     gene            41116..41730
                     /gene="cysC"
                     /locus_tag="PLZ72_01315"
     CDS             41116..41730
                     /gene="cysC"
                     /locus_tag="PLZ72_01315"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNA34962.1"
                     /translation="MPAGDPTAAVSPGVVWHDGAVSRDERAAVTGGRGMVVWFTGLSG
                     SGKSSVAVELERQLISSGRAAYVLDGDNLRHGLNGDLGFSSRDRGENVRRVGEVAALL
                     ADSGTVALVPLVSPYRDDRDRVRRRITSAGLDFLEVFVDTPIEICEERDPKGLYARAR
                     AGELTGMTGVDDPYEAPSRPELVLRPSDGDAVAMAEIVRARLRS"
     gene            complement(41753..42067)
                     /locus_tag="PLZ72_01320"
     CDS             complement(41753..42067)
                     /locus_tag="PLZ72_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34963.1"
                     /translation="MAASSSTSTVDRPSQRTAPRRSRAVLRDVEPGSTVECAHCDERV
                     KFQAKVRNRQVICNVYIKGVWDRVEHFHADCYDLAGEPHGPVDTTPVVRNRARAAEAK
                     TA"
     gene            complement(42188..42529)
                     /locus_tag="PLZ72_01325"
     CDS             complement(42188..42529)
                     /locus_tag="PLZ72_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34964.1"
                     /translation="MDPRIVRRLVLVVFVGGIAGMIVGSIADNNGTAITFGLVTAVAA
                     VCLILVTSVTTRDDSVAFDEARAQAMEAQIQTLVAEGADEAQVRELVRDAVQLGRRAR
                     AVRPARRGPRA"
     gene            complement(42540..44240)
                     /gene="ettA"
                     /locus_tag="PLZ72_01330"
     CDS             complement(42540..44240)
                     /gene="ettA"
                     /locus_tag="PLZ72_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNA34965.1"
                     /translation="MGAQFIFTMHRVGKFVPPDREVLKDISLSFYPGAKIGVIGANGS
                     GKSSLLRIMAGVDDDHTGEARLTPGFTVGLLEQEPRLDESKDVVGNVMDGVAEVAGLL
                     TAYEELMARWSDPDADYEKLGNAQAELEARIEAAGGWDLQRTIDIAMDALRLPPGDAD
                     VSTLSGGERRRVALCRLLLSKPDLLLLDEPTNHLDAESVAWLERFLQDYPGTVVAITH
                     DRYFLDNVARWILELDHGKGHPFEGNYSGWLEQKQERLGRQEKQNEARRRTLERELDW
                     VRMAPKARQAKSKARLSAYEELQKEAEEARDTSSRLEIAIPPGPRLGDQVIEASALRK
                     GFGDRLLIEDLSFSLPRAGIVGVIGPNGAGKTTLFRMIVDAATSAGDPELRPDDGTLV
                     VGSTVELAYVDQSRDSLDDAHTVYEEITDGQDVLTVGTREVNGRAYCASFNFKGSDQQ
                     KPVGELSGGERNRVHLAKLLRRGGNTLLLDEPTNDLDVDTLRALEEALDSFPGCAVVI
                     SHDRWFLDRIATHILAFEGDSQVRFFEGNFSEYEEFRRRELGADASQPHRIKYKPLTR
                     "
BASE COUNT         6307 a        15139 c        16383 g         6760 t
ORIGIN      
        1 ggcccgggcc gggctggacg ggacagggcc ggccggggtg gggctggccg ggacagggct
       61 ggcccgggtg gtcggggtga cggggtcggt gggcaagacc accaccaagg acctcctcgc
      121 caacgtcctg gcccgacgct tcgccacggt cgcctcggag aagtcgttca acaacgagct
      181 cggcgtcccg ttgacgctgg ccaacgcccc ggccgggacc gaggcggcgg tgatcgagat
      241 gggcgcacgc ggcgcgggcc acatccggtt cctctgcgag atggcccgcc ccaccatcgg
      301 cgtggtcacc gtcgtcgagg gcgtgcacac cgaggtgatg ggtccgatcg accagatcgc
      361 ccgagccaag cgggagctgg tcgagcacct tcccgccgac ggcctggcgg tgctcaacgc
      421 ggccgatccg aacgtcgcgg cgatggcggc ccacaccgtc gccccggtgc tgagcttcgg
      481 gaccggggga gaggtccacg ccgaggcgat cgagctcgac gacgagctgc gcgcccggtt
      541 cacgctccgg tcgtcgtggg ggccgaccga ggtgcacctg ggcgtgcggg gcgcccacaa
      601 cgtgaccaac gcgctggctg ccgccgcggt ggggctgtgg ctgggcgtgg acccccggga
      661 ggtggccgcc ggtctggccg aggcgccggc gtcgccgtgg cgaatggagc tgctgcgggt
      721 cccgggcggc ccgacggtgc tcaacgacgc ctacaacgcg ggtccggcgt cgatggcggc
      781 cgcgctggct gcggtggcgg ccctgcccgc tccgggcgcc gggcgccgca tcgccgttct
      841 ggggctgatg gccgagttgg gcgacgaggg tccggccgag caccgacgca tcgccgagct
      901 ggccgacgaa ctgggcgtcg aggtcctggc cgtgggctgt ggtctctacg ggtcaccctg
      961 gacggtggac tcggccgacg acgtcgtcgg ggccctgggg aagttgggcc ccgacgacgt
     1021 cgtgctgatc aaggggagcc gagtggcagg cctggaacgg gcggcggagg cgctgactcg
     1081 tccaggtcga tgagcagccc cagcgcggcg gcgaccaggg ccccgaacag caggaccggc
     1141 acctcgccga tcgagtcgat gaccggaccg gcgatcagcc cgccgacggg caccagcccg
     1201 gcccagccca tcatccacag gcccatcacc cggccccgga ccgagtcgtc gacccgcagc
     1261 tgaagggtgg tcgacagggc ggtgaccagg atgaagtagg cgccgccggt gacgaagacg
     1321 gcggggaagg cgacccaggt cgaggtggtg aggccgaaca ccgccagagc tgcggcgaac
     1381 acccagatgc tcccggtcga catccgtgag cggggcagct cggagaagac ggtgcccatc
     1441 gagatcgctc cgagggctgc gcccagagcg aacgaggcga acagcagcgt gtaggcgatg
     1501 ccgtcgatgc ccaggcgctc ctcggcgatg aggggcatct ggtagatgaa cacgagcgag
     1561 cacagcgaga aggtggagat cgtgatcagg atccgggtga cgacgccgtc ggcccgggcg
     1621 gcggcgacac cctcatggat ctgacgccac ggcgaggtgc cggagtggcc cttggggctg
     1681 aagtcggcgt gcacggtggc gacggcccag accacgaaca ggtaggtgag tccgttggcg
     1741 gcgaacacca gcgccgggcc gccgatcgcg gcggtgaacc cgccgaggat cgggccgacc
     1801 acccggctgg tgttcatggc cgccgagttg agggcgactg caccctggag gtcgtctcgg
     1861 ccgacgagct cggggagcat ggccgagaag gtcgggccgt tgatcgacgc gccgaccccg
     1921 atcccgaaca cgatgaggac cagcgcgatc cggttgggct ggtccgacag ggcgacgacg
     1981 gccaggacca tggacaggac ggtctggctg atggcgatgg cgatcatgag gcgtcggcgg
     2041 tcgacgcggt cggcgagcgc gccaccgatg ggcgacagca ggagcatcgg tccgagctgg
     2101 gcgaaggtga tgacgccgat gaaccaggcc tcgccggtga gctggtacgc cagcacgccg
     2161 agggtgatgt tctgcatcca ggtgccgatg ttcgacgcca ggttgccgta ccagatgcga
     2221 cggaaggcag gctgggagta ggccgacgag agggtgccgg gggtccggtc ggagcgacgg
     2281 ccgccacccg aaaagtcttt cgatccccga agtacctcca taggggttca ggatagccag
     2341 atcgcgggcg cgacggcggc ggagttccgt gtgacgtgtg ccccacggcg agggcgagca
     2401 ctgcacgggt cgaccgctgc gggggtggtg gggcacggcg agcgggccgg ggccaagatg
     2461 caggggtggc ggaacggacc cactaccagg tgctcggggt gcggcagtcg gcgccgctcg
     2521 ccgagatccg cgggtcgtac cgacagctag cgtaccgact gcaccccgac cgccaggccg
     2581 gcgtgaccga ggccgaacgc cagctggccg agcgccgcat gcgcgaggtc aacgccgcgt
     2641 ggacgaccct gtcggatccg gccaaacggg ccgcctacga ccggacgctg gtgccgcgcg
     2701 gctccggcgc atcggggtcc ggctccggaa cgcgggccgg tgccggatca acggcgggag
     2761 ctggtgcgag cggtcgaccg ggcgcgagcg acgcggggtc cggtcagtgg tgggacgcgg
     2821 acgacccgga cgcggtgctg gcccgggcca aggcggccga ggcggccgag gtcgacgctc
     2881 tcgacgagga gccggacctg agcggagtgc agttctggtt gctgcgccgg ggcgtggtgg
     2941 tggcgatggt gatcgtgggg ctgatcatct tcgtggcgtc ggcctacgcc ggccggggcg
     3001 tcaccgatcc gacggcgacg tcggctccgc cggcgatggt ggccgactcc gagtgcgtca
     3061 aggtgatggc ggaccacatg gcctacaacg tgtcgtgcga gggcagctac gacgcgcgca
     3121 tcgtccgaga ggagccgaac gcgcggagct gtctcgacga cggcctcgag tacgcggtcg
     3181 tgcgcgcccg gtccgtctgc gtggacgagg acgtgtaggc gggcatcctg gggaggtcca
     3241 ccccgagggg gtggacggac tcaggccgcg gcgggcatgg gtcggccgtg gcgttgggag
     3301 cccaggaccg cgccggtggg cgtggtgaac tcgaaccact ggtgctcgac ggtccgcatg
     3361 gcccaaccgg tccggtggac cacgccgtgg tggtggcgac acagcgacgc gaggttgtcc
     3421 atgtcggttc ggccgccggt cgcggtgcta tcggtcgagg tgccgcgggt cgaggtgtcg
     3481 tcggcgccgg tgtggtggca gctgtcggag cagacgtcgg agtggtggcc agtgctggag
     3541 gatccggtgg ggacccacgg atcgacgtga tggaggtcgg tgcggctgga cggggcgtcg
     3601 cagccgggga agacgcagcc cccgtcgcgg acccggctgg cacgacgctg gtccctggac
     3661 ccgaaccgct gctcacgtcc caggtcgacg gcgacgccga accggtcgaa caccacgggg
     3721 tggaacgccg cgtcgcagac ggccacctgg gcggtgtagg agtcgacgcg ctcgccgtcg
     3781 tgggtccgca cgactccggg gtcctcggcg tggatcacga aggtcacgtc ggccgcggtt
     3841 cccggaccct tgtgggttgc cgccagagcg acccggcaga tctcaccgag ggccagggcg
     3901 cggatcgccg agtggtgcgg gaggggtagg tcctcgggcg cctgggcggc gtcggaggac
     3961 agctgggcga acacccggtc gctgaacacc gacagggcgt gctccaccac gtggcggctg
     4021 tcgccgacca gcgtcgcctc gaggcgtgcc gagccgtcga actggacgct caggttcagg
     4081 tggttgtcct ccacggaggg ctccgctccg tccggatcgg ccaggcggag cagatccagc
     4141 acctgagcag cccaggcccg gaagagcagg aagtcgtcgg tcatgtcgat cagctgctgc
     4201 tggatcccga tcaccgcgtc cacgttgcgg gcgttggtgg cgttcgcgat gacccggacg
     4261 tggtcgaacc cgatgcgccc cagacgtagg gcctcgtgca ggtcgggaca cgatcggagc
     4321 aggcgagcga ccttgacccg gcggcccgcc tcctcggaag ggaggcgcag ggtcccggcg
     4381 gtccaggtct tggtcgagtg gccgtggcgg cgatcggtgc tctttcgggc gtcgatctca
     4441 ccgttgatcc gtgccgtcgc tgcgtcgagt gctcgtcggg ccgattcgag tgtgacggcg
     4501 aggtcctcga ggccctcgtc gctgatctga tcggggccgg ccgccccggt ggccaggtgc
     4561 cgtgctcggt cgacgatgtc ccggaggtcg gactccagca tcggtgcggg tgagcaaccg
     4621 tcctcggccg gggggcggtc gggtggtggt gtcggcggcg gtgtcagtgg tggtgtcggg
     4681 tcggtcatcc cagctcctcg tccctgtcgt gatcgaacag gtgttcgatc acgacaccaa
     4741 cctatggggt gggtgtgacg gtcgatggtc gtctcgaggg ctctgtctga ggacgtccac
     4801 aggaagaggt ctggtctaga tgtggttcag ctccggccgg gacgtcccgg ttcacccagc
     4861 agtgcgggtg cgaccgggaa cgagtccggg ttcagcacgg tgggcggcga gggctacagt
     4921 gtcccgtccc gggcgtatgg ctcagttggt tagagcgctg ccttcacacg gcagaggtca
     4981 caggttcgag tcctgttacg cccaccacgc gaaacccctg cagtagcggg ggttccgtcg
     5041 ttttcggcct cctgtcggga ctcggccggg gctgctccat cagggctgat cacgctccac
     5101 ggatcggcga gcggtcctcg acgattcagg gctccatgcg gtccacggtg tcgacccggt
     5161 cgatcgagcg gtcgaacgac gcgacgctgc cgacgccggt gctctcggcg cacgccacga
     5221 ggtaggtctc ggcgaagtcg agccgatcgg tctcgtagac ctcgatcgct cggagcagca
     5281 gcgccgggtc caccgtcacg accgatcgca tcgacaccag ggagcgcatc gcggtggcga
     5341 cctggtcgcg tggcgcctcg tagaacgact cgagcacgta gaccgtctcg gcgacgatca
     5401 ggtccgtcag gtacagctcc tcggcctcgg ccaggtaggc cgtcgctcgt gctgccatgt
     5461 ccgcggggtc gcccgtcagg tgtcggacga ggatgttcgt gtcgacgaag gcgctcaccg
     5521 cgccgtcgcg gcccgcttgg cgcgggtcga gcggatgacc tcgtcccacg cgacattgcg
     5581 cttcggtgcg ggcacggcga tggtgccggc cagcgacagg aactccggag tgcgtgcgag
     5641 cacggcccgg tcgccctcga cgcggaacac cacgtcgtcg ccctcgccca gcccgagcgc
     5701 gtcccgtacg gctttgggaa cggtcacctg gcccttggag ctcatcctcg ctgcgatctc
     5761 caccggagac tccttaccat cgcgatgatg taaggatacc cgagcgctgg cgctcgaacc
     5821 gttcggcggc gacggcccgc catcgcgcgg atctcgcccg tggtccggcg taggaacgcg
     5881 gccgcagcct gaccaacagc gcacggcggc ggcacggatc cggcggtagt ggcatcgccc
     5941 ctcgttacat gtaacatggc gtccatggcc cggcgatcca gcatcccgac agccgaacgc
     6001 gttcgtgcct accgcgagcg cctgcgcgcc cagggtctgc gtcctgtaca gatctgggtt
     6061 cccgacgttc gctcaccgca gttcgtcgaa caggctcacc tgcagtcagc tgcggtcgct
     6121 gcgagcaccc atgagtccga cgaccaggcg ttcatcgatg ccctcggctc ggacgacggg
     6181 tgaggcgcgg ggagatctgg accgcagccg cgggcagtgg gtacgtcggc aagccacggc
     6241 cggtcgtcat catccaggac gaccgcttcg atgcgacggc atcggtgacc gtctgcgcgc
     6301 tcacgacgga tcccaccgaa gcgcccctgt tccggctgcc ggtgactccg gacgagacga
     6361 acggaatccg ggagcccagc aacctgatgg tggacaagat cacgacagtg ccacgcgcca
     6421 agctcggcga gaagatcggc cgactccgtg acgacgacat ggttcggctc gggcgtgcgg
     6481 tcatcgtgtt cctgggcgtc gcaggtagct gacgcctcgt caggctcgcg gctggcacaa
     6541 cgtcgaactc cgcgggcttc agcggcgttc ggccgatggt cagcacctgc gcaggctggg
     6601 tctcacgtcg gcacgcggtc cgtctcgtag gcccacatgg cgagctcgac ccggttgcgg
     6661 gcgcccagct tgttcatcag gctcgccagg tgggtcttca cggtgctcag gctgatgcag
     6721 agcgcatcgg cgatctcggc gttcgtgcgt ccgagggcga cggtgaccag cacttcctcc
     6781 tctcgctcgg tgagcggggc gatcggttgc gccgccggct cccgcgtcga gtcgcccagc
     6841 ccggagaagg tcgacaagag gcgtgcggtg acgctcggag cgatcagggc atcaccgttg
     6901 gcggcggccc gcacggcctg ggcgagcagg tcgggcccgg cgtccttgag caggaacccg
     6961 cgtgcgccgg ccttgagcgc cccgtggacg tactcgtcca ggtcgaaggt cgtgatgacc
     7021 acgacggcga gcgggtccgt cacgtccggc ccggccagca ggcgggtggc ctcgatgccg
     7081 tcgagctcgg gcattcggat gtcgagcagg cagacgtcgg ggcgcaggtc ctcggcgagc
     7141 tcgaccgcct cacgcccatc gacggcttcg gcgacgacct cgatgtcggg ctcggcgtcg
     7201 aggatcagac gcagcccggc gcggacgatg cgctggtcgt cggcgatcag gacgcggatg
     7261 gtcatgtcgt caccgcctgc cgcggcaggg tcgccgtgac ggcccagccg ccgtcggggc
     7321 gcggacccgc ctcgaaggtg ccaccgagca gcctggcgcg ctcggccatg ccgatgagcc
     7381 cgaagccggt ggcggtcgcc gtcgatgcaa caacgccccg accgtcgtcg acgacggtca
     7441 ggtggatgtt gtcgtcgtcg ccgtcgacgc gcacctccac ctctgacgcg tgccgggcgt
     7501 ggcgcagagc gttggtgacc gcctcctggg cgagtcggaa gcaggccgcg tcgaccgacg
     7561 accggacccc gtcgaggtgg ccggcgatcc tcaccgtcac ccgggggccg ctgagcccgc
     7621 cgtggcgttc gagacgggac aggtcgccga ttccctgctg tgggcggagg tccgtccggt
     7681 cgtcgcgtag cgctccgacc atcgaccgga gctcctcgag cgcccgcgag gcctcctctt
     7741 cgatcacctc cagggcctcg agcgccccgt tcgcgctggt ggcagcgacg acccggccgg
     7801 cctgggcctg caccgcgatg gccgagacgt ggtgggccac ggtgtcgtgc agctcgcggg
     7861 ccagctctgc ccgctcgcgg gatcgccggg cctcgacctc ttcgacgcgc agctcgaccg
     7921 agtagcgcac ggcgacgccg atcgcgccgg cgagcagcac cacgagggtg ccgccgagca
     7981 cgtagccgaa ttggtcgccg gcgacgaccg acagcgcgat cgggaccgcc acgacgccga
     8041 ggccgatccc gacgtcgcgc cccgacgccc accgggccag cgcgtacggg agcacgagca
     8101 tgaaggcggc ggcgttcagg ccgtcccact ccacgtcgcc gaggatcgag gcgacctcga
     8161 tggcgctcac cgtcccgaac gagaccgcga caaccaggag cggccgaacg cggcgccacg
     8221 gcaacaggaa cgccaggccg gccgtcgtca ccagcgagac ggcgggccac acgagctggt
     8281 cgtcggtgaa cgtctcgacc gtcgcggcaa cgaccacgac gaggacgaac agccagtcgc
     8341 gccgtgcggg cccgggggga tcctcaacgc gtggttgcgc ccagggccga cggctcgtct
     8401 ccagcgccac gccgacaggg tacgagaccg cgggacccca gcggatcggc cgaaagtgcg
     8461 atctgcgggc cagccgacag gaggatgagg aagtgaccgg actcccgatg cgcggactgt
     8521 cgccgatcgc tgacgatgcc gtccatgtca gttccaccga acccgtcccc acccgaccag
     8581 tgcccacacg accagtgccc acccaccccg gctggcgtcc tcggccaggc ccgaacgtcg
     8641 cccggagccc cgacgtgatc gaggtcagag gactcaccaa gcactacggg tcccggcgcg
     8701 ccgtcgagga cctcagcttc gacctggagg ccggtcgggt caccggcttc gtcggtccca
     8761 acggtgccgg caagtccacc accatgcgga tgatggtcga cctcgcccgt ccggaccggg
     8821 gcgaggtgcg ctacgacggg ctccgctacg cggatctcgc ccagccggcg cggaccgtcg
     8881 gcgccgtcct cgacgcccgc tgcatgcacc cgggtcgcac cgcccggaac cacctccggg
     8941 ccatcgcggc gctcagcgcc atcccgggct cccgggtcga cgaggtgctg gccgacgtcg
     9001 gactggagag ggtggccggc gagcgggccg ggcggttctc gctcggcatg cgccagcggc
     9061 tcgcgctggc aaccgcactg ctgggcgacc ctcgggtcct gctgctcgac gaaccgtcca
     9121 acggcctcga ccccgacggc atccgctggt tgcggaaccg actgcgtcgc ttcgccgaca
     9181 acggcgggac ggtgctcgtc tcgagccacc tcatcgccga gctggcccgg ttcgccgacg
     9241 acctggtcgt cgtcggaggc gggcgcctca tcgccgccga gaccgtcgag tcggtcacgt
     9301 cccgaaccgg gatgtcgctc gaggacagcc ttctcgagat gaccgcgacg tccgccgagt
     9361 tcgcctccgc atgaccgccc acgtcgccac cggcgatccc gaaccgtcca ccgctcacga
     9421 ggagaccgac atgaccacca tcgacgcgcc gctcaggccc acccccgagg cccccggggc
     9481 ggccgacgcc cccaacgcca cgggcccacg ccccgctcgc cggttcgctg ccgtcctgcg
     9541 cagtgagtgg atcaagtgcc acaccgtgtg ggccacgaag gccctgctcg ccgtcgcggc
     9601 ggtcgccggg ctggtgatct cctgggccac ggccgccttc tccaccggcc cggtcctgac
     9661 cgccgccgag gtcttcgtct acccgacgct gctgaccgcg gtgctggccg ccgtcgccgg
     9721 catcctgctg ttcaccagcg aggtccagca cggcacgctc gccgtcaccc tcgcggccca
     9781 cccgtcgagg tggggcgtcg tcgccgccaa gaccgtcctc gccgcggcca tcggcgtggt
     9841 cctgggagcg gtcggcatga gtgccggatt cctgggctcg cggatcggag gtctcgacgt
     9901 cggtgacacc tcgggactcg cggcgaaggt catgtgggcg ctgctctaca ccgtcggcgc
     9961 cggtctgttc ggcctcggag tggggatggt ggtccgccac agcgccggtg ccgtctccgg
    10021 cctgctcgtg tggtggctgg tggtcgaggg cctcgtcgtc agcttcgctc cggccgggct
    10081 cgtccgcttc gtccccttcg atgcgggagc ccggacgttg gacatcgcga cggagctcga
    10141 ccgtcccgag gtcgtcgcgg cggcgttgcc gaacccgctg cacgccctga tcttctggag
    10201 ctacgtcgcc gccgccgtcg gtctcggagc ggtcctgctg cgtcgacgcg acgtcggctg
    10261 aggcgatcgc cgaccgacga tggctcgggt gtcgaccgaa cagcggaccc ggccatactt
    10321 cggcgatgca ccgcaacgtg gagaccgtga tcgccgcggc cggcgagaag gggctggcga
    10381 tcgagccgcg cccgttcccg gaggggacga agaccgcgca ggacgcggca gcggccatcg
    10441 gcgtcgaggt cggccagatc gtcaagtcgc tggtgttcct ggtcgacggc gctccggtca
    10501 tggcgctggt ccccggtgat cgacgactgg acgagtcgct gctggccgaa gccgtcggcg
    10561 ggagcgtcgt cgaacgcccc gacgccgacg cggtccggga cgctgtcggg ttcccgatcg
    10621 gcggggtacc gccgatcgga catgcgcttc cggtctacat cgaccggggg ctgctcgcct
    10681 acgacgaggt gtgggccgcc gcggggacct ggaccgacgt gttcgcggtc gcccccgacg
    10741 acctgctgcg gatcacctcc gcgactctgt gtccgttgtc ggagaccgag tagcccatcg
    10801 cgtcgctcag cggctcgatg ggcgcggccc gcgccgtgcg acagaccgcg gtcggtgcga
    10861 gaccaggatg gaaatcgggt gtcggtcggg gccaggcgcg gcaagagtgg gtcgatgatc
    10921 ctgcgccggg cggtccccga cgatgcgacg gccgtggccg agatgcacgt ccgctcctgg
    10981 caggtcggct atcggggcct cgtgcccgac gaggacctgg acacgctccg gcccgagtcc
    11041 tgggccgacc gctacacctt cggttcggac gcggcgagcg atccgatgac catcgtggcg
    11101 ctccgtgacg acgcggtggt cggcttcgtg acgaccgcgc cgaccttcga cgacgacgtc
    11161 gatggctcgg ggcagctctg cgcgctctac gtcgatcccg cggcgtgggg gagtggcatc
    11221 ggctcgacgc tcctgcgggc gggacggaag aacctgatct accggggctt caccgacgcg
    11281 gtgctgtggg tgctggtcgg caaccggagg gcggagcgcc tctacgaacg tgaccgctgg
    11341 cgacccgacg gcgctcgtcg aactgaggag atccggggga tcgaggtcga cgagcgtcgg
    11401 ttccgtcgcc ggctggtgtg agttgccgtg gcgaccgacc gtcgatgggc gatactccga
    11461 cgatggagcc gatcggagtt caccacgtgg cggtcaacgt caccgacgtc gaggcggcgg
    11521 tcgagttcta caccgcggtg ctgggcggtc gggtccgtga tgaccgaccg gacctcggca
    11581 tcggcggggc gtggatcgac ctgggggagg cgcaggtgca cctggtggag ctgcccgtac
    11641 cgccgtcgat gggtcagcat ttcgcggtgc gggtcgacga cctcgacgca gcggtggtcg
    11701 agttgcgtgc cggcgggtac gacgtggccg acccgtcgca ggtcggtgcc ggtctccaga
    11761 cgttcctgac cgacccgtcg ggcaacaccg tggaactgca ccaggtcctg gaggggttgt
    11821 agtccgcccg tgacggaatc ctcggcctga tcggtgctca ggtcggcttg ggttccttgg
    11881 ggatgccgag gatccacgtc ctccgacccc gcccaccgcc cgtggcggaa tcctcggccg
    11941 gccgtcagcc gggcttgggc tccttgggca ggccgaggat cctctccccg atgatgttgc
    12001 gttggatctg ggaggtgccg ccggcgatcc gggcgccggg ggcgctggtc aggccgtagg
    12061 cggcgtcgcc gtcgagcatc gcctcggggc ccgccagcga cgaacgcagc agcgccgtgt
    12121 cgaacatcag ctcggtcgtt cccagcttgg ccaccgatcc cagcaccgac gcgaccggac
    12181 cctgacggcg ccccatcgcc cacagggcgg tgccccggat ccacagctcg gcgagctcct
    12241 gctggtccag tgcgtcgagg tcgccgccga gggccagcat cgagtcgagg cgtcgcttga
    12301 gcgagatccc cgacgcaccg atgtatcccc gctcgttggt cagagccgac atgccgacca
    12361 tccagccttc gttctgcggc ccgagcagcg cgtcggcggg caggcgcacc tcgctcagga
    12421 acacctcgtc gaactcggcg tctccgttca tctggcgcag cgggcgcagc tcgacgccgg
    12481 gggaggccat gtcgatcagg aagaacgaga tgcccttgtg ggggcgggcg tcggggtcgg
    12541 tgcgggccat gaggatcccc cggtccgcca cccggccgtt ggagcaccag accttctggc
    12601 cgtcgacgac ccactcgtcg ccgtcgcgca ccgcccgggt ggacaacgac gccaggtccg
    12661 agccggcgtc gggttcggag aacagctggc accagatccg ctcgccggtg aggatcggtc
    12721 gcaggtgctc ggcctgctgc tcgggggtcc cgaaggccat cacggcgccg ccggtcagga
    12781 cgcagccgac catgttgagg aacggcggca cctgggccag ggcgcactcg gtgatccagg
    12841 ccccgccgtg ctcgggggac aggccctggc caccgtgctc gaccggccag gtgatgccgg
    12901 cgaaccccgc gtcgaacaac gtcttctgcc agcggcgacc ctcggcggcg agcccgggcg
    12961 gcaggatcgc cccgtagtcg cggggcgcgt cgcctcggtg ctcggccaac caggccccgg
    13021 cccgggcccg gaactgctcg accgactcgg ttcctgcgct gctgtccatc tccgctgcga
    13081 cctcccgtga gcgggtgccg ggggaaactg cccgcttggc ccgtcccccc gccagcccgt
    13141 accctctgcc cccatgacga ccgtgggggc gacgtcgtca ccgtacgtgt ccgaggcacc
    13201 tcggctgggt ttcgtcggtg gcttcgatgg tgttcggggg atcgggatcc tgatggtcct
    13261 gtccaaccac gcctattcgg cgctgtcacc gagcttcgcg gggatcatcg acgttttctt
    13321 cgtgatgagc gcgttcctga tcacgacgct gctgctgcag gagcaccggg accagaacac
    13381 gatctcgatg cggaagttct actcccgccg tgccatccgc ctcctgccct ctgcgtacct
    13441 gtgcatcgcg gcgtggctgg tggtcacgat cctgttcgcc cgggaccggc tcaccaccct
    13501 gctggccgag tccgcggccg ccgtcaccta cctctacgag atcttcttcc cggtcggact
    13561 cgggcagctg gacccgaagc tggtcgagca cctgtcgatc gaccagttct ggtccctggc
    13621 ggtcgaggag cagttctacc tgctgatcgc ggtgaccgtc ctggtgtgca tccgcaagcg
    13681 gtggatggtg cagctggcgg tcgggttggc cgctgtggcc accttcatcg gtgtgcagcg
    13741 ctacatgggc atcgccgggc cggttccgat cccgtcggac ccggcgcacg ccaacccggt
    13801 ggtgcggggc ctcagcctgc tgtggttgtc gcggccggac tcgctgatgt ggggtgtcgg
    13861 cctggccgtg ttgaactcga agctgcccga gccgctgccg acggtgtggc gtcgctggtt
    13921 gccccgcgtc gggctggtcg gtctggtcgt gtcgggcgtc tcgatgctgt tggccagcgg
    13981 cttcctgttc gacctggcca acaaggtcgg ggtgccgtac ccgttcgtgc cgatcggacc
    14041 gctcagcatg gagcaggccg gggaccgcat ctactggatc aacttcggcc acacgctgac
    14101 cgcactggcg ttcgcccccg ccatgctggc catggcccgc gtcaaggagt ggtggcccaa
    14161 ccgggcgttg tcgtggaagc cgctgcggtt cctcggtcgc atgtcctaca cggtgtacgt
    14221 gtggcacacc ctggtgtact tcatcgtctt ggatctgctc ggcggcaacg agcgcctggg
    14281 tgagaagtgg cgggtgccga tcctggccgc catcgccatc gtggcctgcc tgccggtgta
    14341 ctacggggtc gagcagcgga tgctgcgggt gaagctgcgc tacgccgccg agaaggaggt
    14401 cctggacctc aacaccggca agatgatccc ggtcgaacag gctcgggtga tgcaccgggg
    14461 catggtgtcg gccgaggcgg tcggtgacgg actcgatggc gggccggccg acatcgcacc
    14521 ccacgacgag gggactcggt gacgacgacg acgaccgctc gggggccgtt cgtcagctcc
    14581 gcccccaagc tcggcctcgt cggaggcttc gacggcatcc gcggcatcgg cgtgatgatg
    14641 gtcctcgtcg gccacgcgct gttcgtctac ttcgagtcgc tggtcacgat cgtcgacacg
    14701 ttcttcgtgc tcagcggctt cctgatcacc acgctgctgc tgcaggagca ccgcaacacc
    14761 ggcgacatcg gactgcggaa gttctacgcc cggcgtgccg tccgcctgtt gccgtcggtg
    14821 tggctgttct gcgcggtgtg gatcgtcatc ggcgtcatcg gcaccgtgct gcacatcgag
    14881 gccatctcgc tgaagggcat cctcgaggac tccgcggcgg cagtggcgta cgtgtaccac
    14941 ctgttcttcc ccaacggcct ctacatgatc gagcccgacg tccagaacaa ccgctacatg
    15001 tggcacctgt ggacgctgtc ggtcgaggag tggttctact tcgccatccc caccacggtg
    15061 ctggtgtgca tccggcgcaa ctggatcaag cagctcggct ggctgctggg ggcggggatc
    15121 gtggcgatcg gcgtcgcccg ctggttcgcg tacaccggct tctggcagga cgacgccggc
    15181 atgatctccg ggatccgcct ggcgctgctg caacgtcccg acggcctcat gctcggcgcg
    15241 ctgctggcga tcgtcaacgc gcacctcacc gcggagcgca tcgaccggct gcgcaagccg
    15301 atcgtggtgg tggcgacggt cggcctggtg gtgtgggcgg tcgcgctcac gacctcgttc
    15361 ggactgttcg agaagctggg cctgccctac ttcaagtacc tgcccgacgg tccggagcag
    15421 ttcagtcgtc cgcagatgct ggcgcagagc ccggtctact ggttccgctg gggtcacacc
    15481 ctcggcatct tcgccttcgg ggcgatcctg ttcggcctgg tgcactgccg gaggtggtgg
    15541 ctggcccggg cctggtcgtg gcggccgttc cagtggatgg ggcggatgtc ctacaccctg
    15601 tacgtctggc acgccctgcc gtacgtgttc atcatcgccc tgaccggtgg cgaggaggcg
    15661 tcgccgacgg tgcaggtgct gcggctcccg gtgctgatcg ccgccgcgtt cgcggtgtcg
    15721 ctgcccgtct actacttcgt cgagctgcgg gccctgcggg tcaagctgcg gttctcggcc
    15781 gagaaggagg cgctcgacct caccaccggc aagatggtgc gcgtcgagga cggcaaggtc
    15841 tcgtcgctgc cgccggagtc ggaccccggc ggcgctgctg accgcgggcc ggacggaccg
    15901 ccgtcggcct agcccggacg gaccgccgtc ggggtaaccc ggacgcgacc ggggccgacc
    15961 ccgaagggcc ggccccggat ccatccctgg acggggaggt cagctgagcg tctcgaggag
    16021 cttgcggagc tcgcccgagg gatcctcggg gacgatgtag cccgtctcgt cgcggatctg
    16081 ctcgaagttg tcgcggacgt cctcgacgct gtgaccctcc tggtaccaac cgggggtgag
    16141 gccgatgaag tagcgggcga cgacgccgcc ggcgacggag tacacctcgc cgttgaccgg
    16201 gcactcctcc gacgcgaggt acacgacagt gggggtcacc tcggaggcct cgagcttgtc
    16261 gccgagcggg ccgagcaggt cctcggtcat gcgggtcttg gcgaccggcg cgatggcgtt
    16321 gaccttgatg cccttggagc ggccctcgtt ggccaggacg cgggtgaagc cgaccaggcc
    16381 gagcttggcg gcgccgtagt tggcctggcc gaagttgccg agcaggcccg agttggagct
    16441 ggtgttgacg acgcggccgt agcccttctc gcgcatgatc acccaggccg gctgggtgac
    16501 gtagaacgcg ccgagcaggt gcacggcgat gaccggctcg agcagctcgg gggtcatgtt
    16561 gtggaacgtc ttgtcgcgca ggatgccggc gttgttgatg acgatgtcga cggtgccgaa
    16621 cgcctcgacc gcggcgtcga cgatcgcctt gccgccctcg ggggtggcga ccgagtcgtg
    16681 gttggcgacg gcctcgccgc cggcggcgac gatctcgtcg accacgagct gggcggccga
    16741 cgcgttgtcg ccctcgccgc tgacggagcc gccgaggtcg ttcacgacga tccgggcgcc
    16801 gcggcgggcg agctccaggg cgtgggactt gccgagacca ccaccggcac cggtgatgat
    16861 cgcgaccttg ccgtcgaagg tcaggtcact catcgagatc ctctcaggag cttcgtgccc
    16921 acggtcggtc cgagggctcg ttcaggcgac cgtcacgcta gagcggcgat cggtgggcgt
    16981 cgaaacccga ctcccgccgg cgttgttgta gctggcaggt cgctggggtg accgtgggct
    17041 acaagttcgg cggcggccgg cgttgttgta gtgggtgggt cgctggggtg accgtgggct
    17101 acaagttcgg ctcggccggg gttcagtcga gcgggatgcc gagctcgtca gcggcgtagg
    17161 ccttcatccg cttgtagtcg accttgccgt tgggggcgcg gccgatggtg tcgatcgaca
    17221 cgacccgctt gggggccttg tatgccgcga ggtggccctt gacgtgggcg atgagtgcgg
    17281 actcgtcgat cggtgaaccg gcggccggct cgaccaccgc cgtgatcgcc tcgccgaact
    17341 tgtcgtcggg aagcccgaca acgacggcgt cggcgaccga cgggtcggtc ttgagcgcct
    17401 cctcgacctc ttcggggaag accttctctc cgccggtgtt gatgcacacc gagccccggc
    17461 ccagcaggct gatcgtgccg tcggcgtcga cggtggcgta gtcgccgggc atcgagtacc
    17521 gcacgccctc gaactcgatg aacgtcgcgg cggacttctc ggggtccttg tagtaggcga
    17581 tcggcacgcg acccggcacg gccacccggc cccgctcacc ggagcccgcc tcgaccggct
    17641 tgccgtcgtc gccgaagacc tggcatcccg ggccgagggc gaagtgagcg gtcttctcgg
    17701 cgttctcgcc ggccgagatc gagctgccca tgccgatcgc ctcggacgag gagaacgtgt
    17761 cgaccaggaa catgccgggg ttgtgggcga gcatcgcctc cttcaccggt tcggaccaca
    17821 tcacgcccga ggacgcgacg agcatcaggc tggacaggtc ccagtggccg ggccgggcgt
    17881 cgagcgccct caccatcggc ttgccgaacg cgtcgcccac gatggtgagc gcgccgattc
    17941 gctcgcgctc gatcgtgtcg aggacctcgg tccagtccag gctgcggtcg accagcgtga
    18001 ccaccgatcc ggcctgggtc aggaacatgt tggcggtgaa ccacccggtg ccgtgcatca
    18061 gcgggcaggc cggaagcccg gcggatccgg gcgcggcgat cgagtcgatg aaggcgtcga
    18121 cgccgccgac gtcggcgggg tccgcggcca gcagcgcgtt gccggtggcg caggtgaccc
    18181 cgatcaggtc gtcctggcgc cacatgacgc ccttgggcat gccggtcgtc ccgccggtgt
    18241 agatcatgac gacgtcgtca ccgctgcgac cccacggcgg gatcgcacgc cccgccgagg
    18301 tggcgaccac ctcggcgtag tcgtcggccc agtcgggtcg ggcgtcggtg ccgtcgtcga
    18361 cccagatcca ctggcgcacc ttcggcagct gcggacgcac ggtggcgacc cgttcggtga
    18421 acgtgccgtg gaagacgacg gccaccgcat cggcgttgtc ccagaggtag accagctcgt
    18481 cgtcgaggta gcggtagttg gtgttgaccg ggaccatccc cgccttgaac gccgcgtagc
    18541 agccctccat gtactcgggg cagttgtaga ggtacagcgc gaccttgtcc tggtggacgg
    18601 cgccgccctc gatcaggtga tgggcgaggg cgtcggagcg ggagtcgaac tcacgccagg
    18661 tgaagcgctt ctcgccctgc acctgggcct gggcgtcggg gaacttgtcc gcgatcgcct
    18721 cccacagctc ggcgaagttc catccggcca ttcgagaccc cctcgggttc ggatcgtgca
    18781 cgggccccgg ctcgccctcg cgggcgggtc cccgtgcggt gacgtgggcc gaacggtacc
    18841 gtccgccggc atggacttcg aactacccgg tgacgacgat ccccgccgag cggcggtgcg
    18901 ggcgtggctg gaggccaatc cggacccgtc gggacgccag ctggccgagg cgggctacgt
    18961 cgccccgcac tggccggcgc cgtggggcct cgacgccgat ccgatccacc agatcatcat
    19021 cgacgaggag ctccgtcggg ccggggtgcg ccgacccaac aacccgatcg gcatcggttg
    19081 ggccggcccg acgatcctcg aggccgggac cgacgcgcac aaggagagct acctctttgg
    19141 cctgctgtcg ggcgaggaca cgtggtgcca gctcttcagc gaacccggcg ccggatcgga
    19201 cctcgccgcg ctgtcgaccc gagcggtgcg cgacggcgac gagtggatcg tcaacggcca
    19261 gaagatttgg acgtcgctcg cgcaccactc ccgctacggg atcctgatcg cccgcaccga
    19321 ccccgacgcc cccaagcacc agggcatctc gtacttcatc tgcccgatgg acctgcccgg
    19381 catcgagatc cgaccgatcg tcgagatgac cggggcccac aacttcaacg aggtgttcct
    19441 caccgacgtg cgcatccccc acgacctgct ggtcggcgag gtgaaccagg ggtggcggct
    19501 ggccaaggtg accctggcca acgagcgggt gtcgctgtcc tcgggcggcg ccctgtgggg
    19561 gatgggtccc gacgcgcagg acctgctgga ggcgatccgg agtgccgggg ggctgaccga
    19621 tccgatcgag cgccagcggg ccgccgagct gcacatcgag tccgagatcc tgcgcctgat
    19681 ccggctccgc acggtgaccg cggcgatcaa gggccgccaa cccggccctg aggcgtcgat
    19741 ccgcaagatc ctcgccgacg agcacggtca gcggatcatg gggttggcca aggacctggc
    19801 cggcccggcg ggcatgctgc cctcgggtgg acccctcggc accgacgtga acctgtggca
    19861 ctacggctac ctgttcgcgc aggcgctgac catcggtggc ggcaccggcg acgtgcagcg
    19921 caacatcgtc gccgaacggg tcctcggcct gccccacgac gtcgacgtcg agcagggtcg
    19981 gacctgggct gagtcccgac ggtccggagg gtgacggtga cgaacggtcc ggcggagcgc
    20041 tgagcggtgg tcgggccccg ggtgttcgtg accggcgggg ccgggaccgt ggccgggtgg
    20101 ctggtccgca ccgcaccggc ggccatcgac ctgcacgtca ccgagcaccg gaccctggtc
    20161 ccggacggcg tgcggacccg ggcctcggtg caccggttgg acctgcggcg acgcgacgaa
    20221 gtggagcgcc ggatctcgga gatcgcaccc gacgtggtcg tgcacacggc ctatgtccag
    20281 gacgagcctg ccgccatcgt cgacgccacc gcggcggtgg ccgcagcggc tgcctcggtc
    20341 ggggcgtcga tgctgcagct gtccaccgac gtggtgttcg ccggtgaccg accgccctac
    20401 gacgagagct cggagccgga ccccgtgtcg gactacggac gctggaaggc cgaggccgaa
    20461 cgccgggccg cggcgatcct ggccgacgtg tgcatcacgc gcacgtcgct ggtggtgtcg
    20521 atcgatccgc ccgaccgggc caccgccggg ctggtcgagg ccctgagggc ttgccgtccg
    20581 atcacgctct tccacgacga gatgcgccag ccgatccggg ccgaggacct ggccgccgag
    20641 ctgtgggcgc tggtcgccct cgaccgggcc gatcgggccg gggtgtggca cctgccgggc
    20701 cccgaacacc tctcacgatc cgacgtcggc ctgcgcctgg cgcgacggct cggactggac
    20761 ccgtcgccgg tcgccgtcgg aaccgcggtg gaccacccga gcccccggcc ccgggacccg
    20821 gcgctggtgt cgcgacgtcg gcaggtgctc ggggtccggc tgcaggccgt cgacgcctga
    20881 ccgggtaccg tgtgcggcga tgacgagcag cactgacaac gacgagctga aggtcaacgc
    20941 caaggtcgtc gccaaggacg acctgcccgg cgtccccgcg gggaccccgg gacgggtgac
    21001 gttgaagaac ggcttccgct ggacccgcta ccgcgtcgag ttcgacaacg gcgtcggcat
    21061 cggttcgctc gacagggccc agctggtgcc ccgcaaggaa tacgaggccc agcagcaggc
    21121 cgccggctga cccgcccggg tccgccatcg actcacatca gcaagccaca acacagttcg
    21181 ggggacagcg tgaagctcag catgcagatc ggctactcgg gagggttcgc cgagtcggtg
    21241 gcccaggtcc aggcgctcga gaaggccggc atggacatcg tgtacgtcgc cgaggcctac
    21301 ggctacgacg ccccgaccct gatgggttac ctggccgcgg agaccgagac gatccagatc
    21361 gcgtcgggca tcctgccgat ctacacccgc acgccgacgc tgctggccat gaccgcggcc
    21421 ggtctcgacg agctgtcggg cggccgggcc gtcctgggcc tcggggcgtc gggcccgcag
    21481 gtcatcgagg gcttccacgg cgtgccctac gacgcgccga tcgggcggac ccgcgagatc
    21541 atcgacatct gccgcaaggt gtggaagcgc gaggagcccg tcgtgcacga cggcaagcgc
    21601 taccagctgc cccttcccga ggggcagggc accggcctgg gcaagccgct caagatgatc
    21661 acgcaccccc gtcgggctga catcccgatc cacgtggcgt cgctcggccc caagaacgtg
    21721 cagatgaccg ccgagctggc cgacggctgg ctcccgatcc tgttccaccc cgagcgggcc
    21781 aaggacgtgt ggggagccga cgtcgatgcg ggtctggcca agcgatccgc cgagctcggt
    21841 ccgctcgacg tcatcgccgg cggcctgctg tcgatcggcc ccgagagcca ggtcggcggg
    21901 ttccgggact tcacccgggg catggtcgcc ctctacgtcg gcggcatggg cgcccgtgac
    21961 cgcaacttct acaaccagct gttccagcgc tacggctacg aggccgaggc caagcagatc
    22021 caggacctgt acctcgacgg caagaagcag gaggcggccg ccgcggtgcc cgagtcgttc
    22081 ctggaggaga cgtcgctgtg cggcgacgag ggctacgtca aggaccggat cgcccagttc
    22141 aaggcggccg gggtcacggt gttgaacgtg acgcccatcg ccccggacct cgagggccag
    22201 cagcggatga tcgccaaggt caaggagctg tcggcctgaa ccgatcccgt gtcgagcggg
    22261 ccccgccggg cccgctcgac acggtcgcac gtcgcaggac gcggaactgg ctgccgtcgc
    22321 accggatcgg atccggggac ggcagccagt tcgcgttccg gggcccgtcg ggcccagggt
    22381 cggatcaggg agcgggcggc gcctcggtgg tggtcggggc ttccgtcgtg gtcggggcct
    22441 cggtggtcgt cggggcctcg gtcgtggtcg tggtggctgg cgccggaagg gtgccgaggt
    22501 ccatcaggtc cgagatgatc ggcatggccc gagacacggt gcagctggcg gtggccgagt
    22561 aggtccagtt gatcagtccc gccttggggc cgatgaacac gccgccgtac tcgacggtgg
    22621 cgggaccgct ctggccgaac tcgatcggtg tgcccttgac gacgccgagg gtaaggaacc
    22681 gctcgccggt gatcgtgttc tcgggatcgg ggttgttgat cgacacgaca ccgatgtgga
    22741 tcgggacgac gaacggcgtg acggtcgcct gttcgacggc catgcccgca ccgtcgccgg
    22801 cgttcaggta gagctcggcg cccagaccgt tggcgttgga gcagtactgg aactcgccgg
    22861 agatctgctt gtccaccgcg agcatggtcc cggcggccgg atcggcgatt ccggccatgt
    22921 ggaagttggt ggtgggcgcg ggctcggggt cctgcgcctg gctgggaagc gtccaggcca
    22981 gcaggccggc cgtcgcgacc ccgaccgcgg cgacaccacg ccgtaccgag cgcgcccgcc
    23041 ggctgcgttc gtgggtgtgc gtccccttcg actccatcgc ccgttctccc cagtgcggtc
    23101 ctgtggtcag gacgtccggt cccgccgacg ccgaagcgcc gacacgtcgc tgcgacgaac
    23161 ccgagccacc gctcccacgg cggcagggga cccacagcag ctccgaagtt accagctggc
    23221 gtggcacgag tcacgcgttc gggcccgggc ggggcccgac gggtccggcc ggggagcgag
    23281 ttgccgggcc ggctcgctca ggacaggccg gcgaggaccg cctcggcctc ggcgaccatc
    23341 gaccggacga tgtctcccgc cggaaccagc tcgtcgatcg cgccgatggc ctggccggcc
    23401 ggcatgaact cccggtcggg gtcgacgccg ggggtgtcca ggtctccgcc gaggtggttg
    23461 gcgccgtcct cgaccgaacg gacgtactgc atcgggaacg gctcgggcac cccgcccgac
    23521 tcctcgaagg cccgtgcgta gccggtgcgc aggacccgac acgttttgcc ggtgtaggcc
    23581 cgggtgatga tcgtcccgtc ctctgccgat gccaggatcc gctccttgta gcccgccacc
    23641 gcctgagcct cgggcgtggc gatgaaccgg gttcccaccc agacccccac cgccccgagc
    23701 gtcagcgccg cggccaggcc ccggccgtcg aagatcccgc cggcggcgac caccggcacc
    23761 cggtcgccga ccgcgtcgac cacctgcggc accagggcca tcgtggcgat ctggccggtg
    23821 tggccgccgg cctcggtgcc ctgcgcgatc accaggtcgc agccggcgtc cgcggcccgg
    23881 accgcgtggg cgaccttgcc gcacatgctg gccaccacga tcccgagctc gtggcagagg
    23941 tcgatgacct cgccggggac gccgagtccg gcgacgaaca gagagccgcc acccgcggcg
    24001 atggcgtgga ccttggcggt catgtcctgc ggggcggcgg tgagcaggtc caccccgaag
    24061 ggacggtcgg tggccgcggc caccgccgcg atctgcgcgg ccatctcgtc ggtgcccatc
    24121 gtcgaggcgc cgagggttcc gatgccgccg gcgttagaga ccgccgcggc caacggggcg
    24181 taggagaccc cacccatccc ggcgagcatc acggggtgct cgatgcccag catctcggtt
    24241 agtcgggtct gcatggccgg catcgtacgc gccgaccgtc tccgtcagct ccgataggcg
    24301 ccggggccgc tgagcgcacc ccggtgccaa cgcgacgggg tgaacgcgat gcctcgggga
    24361 gagtcctcga agagccaacg ggcgaagttg cgccgcgtcc acggcgtggc cccggcgacg
    24421 cggatggcgg cccgcgcccc cttgcggtgc tggagggccc gcaccagcag ctgcgacatg
    24481 cggtggtcgg cgacgagctc ctcggtcacc gtgcggtggt agtgggtcgc gacgtctccg
    24541 acagcggccc ggtcccggat cgactcggcg gcccggaccc cggtgagcag ggcctgcccg
    24601 atgccctcgc cggtcatcac gtcgcacgcg gcgaccgcgt cgccggtgaa caggacccgc
    24661 ggcgtcgagg tcaccgcggt gtcgattcga gccgggatcg gccacgcccg gtggggggcc
    24721 tcgggggtga cgtcggggcc gagcaggtcc cggatgtgcg gacgagcgag caggtcctcc
    24781 cacagcggac ccatgtcgcc gaccgggacc tttccgcccc gctggatgcc gaagccgatg
    24841 ttggcccggc gccccggcag cgggaacgac cagaagtagc ccgggacgat gtcggcctcg
    24901 aacatgacgt gcagctcggt cgcggcccgc tcgccgacgt tgccgacgta ctggcggaac
    24961 gcgtgccact cgccccggta gccgggctcg gccaggccga gcgcccggcg agtcggcgac
    25021 cacatgccgt cggccgcgac gacccaacgg gcccggacct cgcgctcgtc gccgccgtcg
    25081 gctccgatcc gcagcacgac ggcgttcgac tcctcgcggg cggcggtcag tccggcgccc
    25141 tcgacgacgg tcgccccctc cttgcgggcc acctccacca gtgcggcgtc caggtcgagg
    25201 cgcgtggtca ccgcggcgaa ccgaccatcg gaggccggcg ggaggggcag gtggatgctc
    25261 accccgctcg gggaggtgac gaagcagtcg ctgacgtcga tccacgaggc gacgtcggcc
    25321 ggatccagac cgagctgttc gagttggcgc agcgcgaggg tcgtgaggcc gtcgccgcag
    25381 gtcttgtccc gcgggaaggt cgccttgtcg aagacggtga cgtcgatgcc ggcgcgggcg
    25441 agggtgatgg cggccgccgt ccccgccggc cccgcgccga tgactgcgac gtcgatctgc
    25501 tcctcggccg gttccacggc gcgcaggcta gttccggctc cgggggcacc cgaccccgtc
    25561 ggagggggtc cggtccgctc aggggcgggt gggcggcgac accgtcttgc gggggtcgcg
    25621 ttcgatcacc ggctccagca cggcgtcgac cgcggcgacc aggtcgtcgt ccagggtgat
    25681 ctccgcggcc acgacgttct cggtcagctg ctcgggtcgt gacgccccga cgatcgccgc
    25741 gccgacgtcg gggttccgca gcacccacgc caccgcgtac tgcgccatgg tgatgcccgc
    25801 cgcgtcgacg agcggcttca tggcctgcac cgcctccagc acctcgtcgc gcagcaggcg
    25861 ctgcacgaac ccggccccgc cgtggtcgtc ggtggcccgg gagccctcgg ggaccggctc
    25921 gcccggacgg tacttgccgg tcagcacccc ctgggcgatc ggcgaccaga cgatctgacc
    25981 gacgccgacc gcggcgctgg cggggaccac ctcggactcg atcacccgcc atagcaggga
    26041 gtactgcggc tggttggaga tgaacgggat ccgcagctcc gccgcgagct cggcggccgc
    26101 ggtgatctgc tcggcgtccc actccgacac gccgatatag agcaccttgc cgctgcgcac
    26161 gaggtccgcg aaggcgacca tcgtctcctc gaggggggtc tcgtggtcgt agcggtgggc
    26221 ctggtagagg tcgatgtggt cggtgcgcag ccggcgcagc gacgcctcgc agctctcctg
    26281 gatgtgcttg cggctgagac cccggtcgtt ggggcccgac ccggtcggcc agtacacctt
    26341 ggtgcagatc tcgaggctct cccggcgctg gtcggccagg gcggcaccca tcaccgcctc
    26401 ggcccgggtg gcggcgtaga cgtccgcggt gtcgaaggtg gtgatgccct ggtcgagcgc
    26461 ctcgtgcacc gccgcggtcg cggtgtcgtc gtcgacctgg gacccgtgcg tgatccagtt
    26521 gccgtacgcg atggcactga ccttgaggcc ggagcggccc aggtagcggt gttgcatgtt
    26581 ggatctcctg gcggttcgga cggggtggac gggacgggcg tcaggccagg tcgaggacct
    26641 cgtcgtagcc cagtcggatg cactcgatga aggagtccgg atcctcgacc gccgcgggat
    26701 ccaggttgag gccgatgttg aggtcgttct cgtagctgag cagggtgacg ttgcagccgg
    26761 ctccagcgag aggcccgaac ggcacgatcg agtccacccg gacgccccgc aggtagagcg
    26821 gcagcggcga gccgggcacg ttggaggcct ggaagtccaa ccccttcatc atcgacccga
    26881 acacctgggt gaccacggtc gtgggcaggc ggttcagcag gttggccatc ggttcgacca
    26941 gctggttggc gggttcgtcc cgggcgctca gcaggcggcg gcggatcgtg cgcatcagct
    27001 cgaagatgtc ttgggcgtgc accgggatct cgaagcgcgc cggcacgaag gcgttgccgg
    27061 cggtgtcggt catctcgccg gcgcggacgt tgaccggcat gcccatgcgg aggtcatgca
    27121 gcggggtgtc gtggcgctgg tggtagtgct gcaggccccg gaccaggccg gtcacgtagg
    27181 cgtcgttgat cgagcctccc acccgcttgg ccgccttgcg ggtcacgtcc agcgggagcg
    27241 tgagcacctc gaggtggttc gacagggaac gccccgacat cagcgccgac agcggatcgg
    27301 gggagggccg caggacgcgg gcggccgagg cggccagctc ggcaccgttg gcgaaggtgc
    27361 cgacgggatc ggacgcagcg ctcgccgcga cgtggacacc ggcggctccc agctcccgca
    27421 ggagcccgag gctcgagcgc acctggtggt ccacggcgtc gttgaaccgc tcgacctggt
    27481 tgagcacgtg caccggcggg ggcggcggca tgtcgcgctc cggtgcgtcg ggttccaggt
    27541 cgaacagctc cagcatgagc cgcaccccgc ccacgccgtc ggtcatggcg tggtgcagct
    27601 tcatgatcag cgcgccgcgt ccgtcggcca ggccgtcgac cgcggtgcat tcccactggg
    27661 gtcgggcccg gtcgaagccc tgcatggcga tgggttgcac catgcgcagc aggtcccgca
    27721 tggacccgtc cccggggacg cgggccaggc ggaagtggta gtcgaggtcg aaggccgggt
    27781 cgacctccca gcggggcggg gccaccgaca tcgggttgga gcggacccgc tggcggagtc
    27841 gggggatcgt cctcgacaga cggtcgaagc tgcggcgcag ctcgccgtgg tcgaccggtc
    27901 gatcaagcag caccaccgcg gtgatcgtgg agcgcagcat ggggtccttc tcgatggacc
    27961 acatgagagc gtcctcgtcg ctcatccggt ggtggtagtg gatgggggtc gcgctcggat
    28021 cgtcgtccac cggctcaccc tacccagctg tacgacccgg gtctcgggct cagctcccgg
    28081 gccctgttcc ccgaccctgc accccgccga gccgactcag atgcagcggt agctggcgtg
    28141 gaagttctgc ttgtcggtgt ggccgtcgac gaaggtgcgg gtgcgctcgg tggtgacctt
    28201 tccgcacccg ccggtggggt tctgggccgt ctgctcgccg cgggcgtagg gcgtggacca
    28261 caactgcacc gtgaccgacg agttggtgta cgtgggccag atcacgaccc cgtacggggt
    28321 gttgttgtgg atcttcaggt cgacgctcgg gtaggcgagg gtggcttcgc ggccgaacgg
    28381 gtaacggctg atgtactcgg agtgggcctt gtaggcgggg atgtcgagtc cggcgaagaa
    28441 cgcggcgttg aacagcgtcg tcgcgtactg gctgataccg ccgccgaagt cctcggtgtg
    28501 ctcgccgttc tcgatgaccg gagccggcac gtagcccttc tcggcggtgc gcttgccgat
    28561 cgtgtcgttg accgagaacg tgtcgccggg ggcgatgagg atgccgcggg tggcgtcgga
    28621 gatggtgtgg atgttgcgga cccggggctg acctgcgggg tggcgggtgg tgaactcgcc
    28681 gaccacctgg gtgacgccca gcgtccgggc ccattcggcg ccctcggccg cggtgacggt
    28741 tcgggtgggc aggtcgacct cggtctttcc ggccagcagc gcgtcggcca gcttctgggg
    28801 agcgtccggg ccgcagcaca cctgggcgtc cttgccgggg accggcaccg ggaccccgcc
    28861 ctggatgtcg aagcgcacgc cggtggggtt gcgcgacccg ggcggcatgt tggtggcgag
    28921 cagttcggcg acgcgggcgg cgtccatgcc gagctgtggg gtcggatgct cgggggtgcc
    28981 ggcggcggtc acggtgaacg ccggccggaa ccgcttgccc tcgatctcga aggtgcgccc
    29041 gcccgaggtc agggtgacct tgccggtggt ggcctggttg gcccgaaccg cgagcgcctg
    29101 caccgcctcg tcggtcatct tcggttcggt gaccgcccgc ttcacctcga tgcggatcgg
    29161 tcggccgacc tcggccaccc ccgacggcag ggcggcggcg accgagttgg tcgtgaggtc
    29221 gatcccgtcc ttgccgggga ccacggcgat ggcgccgtcg gtgatcgtga tcgacggctc
    29281 gacgggatcg gagcgcttgt cgccctgcag ctccaccagg gtccgcgaca acgcggcacc
    29341 gtcgagggtg agctcggcgt cgacggcgcg gggcgagagc acggatccgg cccagcggac
    29401 cgggcgggtg ggcaggggat cgtcgcggcc caccgcccat gcccgggcga cggtgccgtc
    29461 ggtgtcgatc gacatgccga ggtcgccggc ggtgctctcg aggtggaggt ctccggcgtc
    29521 gatgctcacc ttggtcgcgg ggaactcggc ggccatctcg tcgaccgcgg cgccgagctg
    29581 cttgcgggag aggcctccga cggagacccc ggccacctcg acgttgcgcg cgacagagtc
    29641 cgaggtcagc accgcggcgt cgacggcgta cgcggacacc aggacgagga tcagggccag
    29701 gggcgcggcg accagagcga cgagcgcctt gcggggggtt gacaccgcac tagggtccca
    29761 catggtcgcc ccccggtcgc ggcactggca cggctcgttc tcaggaaccg gtcgatcccc
    29821 ggagcgtgac cgacggggtc gtccgagcgg ccagcggtcc gcacggaccg tcaggggcag
    29881 gatttcgagg ccggccagag gatcaggggg acaagatggg catctgcgag ggccgcgtgt
    29941 gcgtggtaac cggtgcgggc cgtggcatcg gccgggagta cgcgctgagc ctggccgccg
    30001 ggggtgcccg ggtcgtggtc aacgacctcg gcggcgagat ggacggcggc ggcaatccga
    30061 cgtcgggtcc ggcgtcggag gtggtcgccg agatcgtcgc cgcgggcggc gaggcggtgg
    30121 ccaacgcgga cgacgtgtcg agcttcgacg gcgcggcccg gctgatcgcc acggcggtcg
    30181 acgcgttcgg ggacctgcac tgcctggtca acaacgcggg catcctgcgg gaccggatgc
    30241 tggtcaacat gaccgaggcc gagtgggacg acgtgatccg tgtccacctc aaggggacct
    30301 tcgccccgac gcaccacgcc gccaacctgt ggcgcaccca ggccaaggcc gccgaggcgg
    30361 gggagcgcga ccggccggtg gcccgggtca tcaacgtcac gtcggtgtcg gggatctacg
    30421 gcaacgtcgg ccagaccaac tacggcgcgg ccaaggccgg catcgccggg ttcaccttca
    30481 tcgcgtcgtt ggagctggcc cgcttgggcg ccaccgtcaa cgccatcgcc ccggtcgccc
    30541 tcacccggat gaccgagggc ctggggccgg agccgaccga ggagcagcgc gagctgatga
    30601 gcccgcgctg gatcgctccg gtggtgacgt ggctcgcctc cgaggagtcc tcacacgtca
    30661 ccgggcgggt gttcgaggcg tcgggccgga tgctggcggt ggccgagggc tggcaccgcg
    30721 gcccgagcgc cgatccggta gaggacccgg cccgggtcgg cccgatcgtc gaggacctgt
    30781 tggcccgggc ccgaccgccg gccgacatga acggcgccga tcggacgacc tgaccgctgc
    30841 cccggggcgg gccggggacg ggggccagcg gcgcctgagc cggaaccagg atccagaacg
    30901 acgtgggccg acggtgaggt cggcccggag aagacccaac cgaggagaca ccgacatgcc
    30961 gatcaacccg gacgccgtgg gcacgcgcag cgacccgacc gagtcgtcgt ggcgcagcaa
    31021 ggacggactg ctctacgcgg tcggcgtggg agcgggccag gacccgctga gcgagaccga
    31081 gcggccgttc ttcagcgaga actcgatcga caccacccag cgcatcctgc cgacgatggt
    31141 ggtgaccgtt cccgatgtca gcggcgcgtt ccgggcgatc gggtcgttca acccggccat
    31201 gctcgtccac ggcgaggagc ggatcgtcct gcaccgcgag gtgccggtgg aggggaccgt
    31261 gtccaccgtg gccgagatca ccgcggtgtg ggacaagggc aagggtgcgg tggtcgagat
    31321 cacctccacg tcgaccctgg tcggcgagac cgagccgctg ttcgaggtcg tcatgagcgc
    31381 gttcatccgc ggcgagggcg gttggggcgg cgagcgcggc ccgtccggtc cgcgccacca
    31441 ggccccggac cgcgaccccg acgcagtggt cacctacccg acccgacccg agcaggcgct
    31501 cgtctaccga ctcagcggcg atcgcaaccc gctgcactcc gatccgggct tcgcggccat
    31561 ggccggcttc gaccgtccga tcctgcacgg gttgtgcacc tacgggttca ccggccgggc
    31621 gctgctgcac gagctgtgcg gctcggaccc ggcccggttc cgcagcatgg aaggccggtt
    31681 ctcgtcgccg gtgatgccgg gcgaggacct caccgtgcag atgtggcgca ccggcgacgg
    31741 tgccgcggtg taccggacgt tggcctcggg cgaccgggtc gtgctcgaca gcggagcggt
    31801 caccttcgac gcctgagcgc tccgtcgccc acaaggtcag cgcggcccgc cgaacttgta
    31861 gctggcaggt cgtgtgggcg accgtggcct acaaggtcga cgcggcctgc cgaacttgta
    31921 gctggcaggt cgtgtgggcg actgtggcct acaagttcgg cggatcagaa cagggtgagg
    31981 tcggcctcgc gagcgccgcg gaggacgtcg tagtcgacct cgacccagtc gatcccccgg
    32041 gcctcggcca gcaccttggc ctggggcttg accacctggg cgacgaacac cccgcggacc
    32101 ccgcgcaggg tggggtcgcg atccatccgc tcgaggtagc ggaccagctg ctcgacgccg
    32161 tcgatctcgc cccgtcgctt gacctcgacc gcgaccgcag tcccgtcggc gtcgcggcac
    32221 agcaggtcga cggggccgat gtcggtcggg aactcccggc ggaccagggt gaggtcagca
    32281 gcgatgacgg tcggatcggc ggcgagcagc tcctggaggt gggcctcgac gccgtccttg
    32341 cgcaggccgg ggtcgatccc gagctcgtgg gcggtgtcgg agacgacgtc gtggaggtgg
    32401 atggtgagct gctcgccctt ggggttggtg acggtccagt ggtcctcggc ctcgacgacc
    32461 acgttggggg cgttcatcca gttgagcggc ttgtaggcgc cgccgtcggc gtggatggcg
    32521 acgcacccgt cggccttgac catgatcagg cgcagagcct cgggcaggtg ggcggtcagc
    32581 cgtccgtcgt agtcgaccga gcagcgggcg atcaccagac gcacgacgcc gaccctaccg
    32641 gacccccgac cccggttctg acgacaagtc acgacactgg cggtactcag atgtcgtcag
    32701 aaacctggag gtcgccggtt ctgacgacaa gtcacgaccg tggcggtact cagatgtcgt
    32761 cagaaacgtg gtgggtcagg ggttcagcgg cgcagggccg ggatgcgcag cgacaggtcg
    32821 ttgcactcct cgcacaccga atcggggacc acgcccgcct tgatgagccc ggcgaagatc
    32881 cggcacccga ggcagaaccc gaaggcggct tcgaggaagg ctgcgccggc gagcatggcg
    32941 atcacgacgc gggccgcccc cgacgcaccg gccagccaca gagcgctggc tccgacggtg
    33001 aacaccaccc cgatgccctg ggcgaaccgc ttgggcggtc cgggcaccag gcgctcgtgc
    33061 tcggccaggc ggggggcgac gacctgggtg gcgattcgtc ccagcgggct gagcgtcgga
    33121 ccggtgagga cccgggcgat gaacccgtag gtcagcggga cgagcagcca gccccaaccc
    33181 gtgacgacga aggtcaccgc catcagcacg accccggcgg ccacggtgcg ggcagcgacc
    33241 tcgttgaccg ggttggggaa ggtgaagatc cccgatgtcc gacgggtcag gtcagtggtg
    33301 gtggcggcca tgggtgcaac ctacgccctg aatcctgacc aaaccagtcg taattgtcac
    33361 aggtcgtcgg gagaccgacg gttccggcga tcacggggtg gctcccgctc gacccgccgg
    33421 agccctcggg gcagtctggg tcggtcctga ccgccttcac ggccgcgccg cgtgcgcaac
    33481 ggaggccccg tgccgagccc gacaccatcc gatccgaacg tcccgcagct gtcgatgatg
    33541 ctgcgcagct tctcggccga ggaccccggc ggctgggagc cgatgctcga gcacgcggtg
    33601 gcgatggacc gcgcgggtgt cgaccgtctc gcggtgtcgg accacgtctg cttcggtgag
    33661 gcgctcgacg cctacggcga tcctgctgtc ggcggcaccg ccggcggacg ccagccgacc
    33721 ggtcccgatg gtcactggct ggagccgctc acggtgctca ccgcggtggc cgcccgcacc
    33781 agtcgcatcc ggctgtccac cgggatcctg ctcgctgcgc tgcgtcgacc ggtggtgctg
    33841 gccaagaccg ccgccaccat cgatgtgttg tcgggtggcc ggctcgatct gggggtcggg
    33901 gtcggctggc agcgtgagga gtaccgggcg gccggactgc ccttcgagcg ccggggccgt
    33961 ctcctcgatc acacgctgga ggtgtgtcgg acgttgtgga ccgagccgcg ggctgaccac
    34021 gactccgagc acctgcactt cgaggggatc caccagatgc ccaagccccg acagcccgcc
    34081 ggggtgccga tctgggtgag tggcacggtc aaccccgctg ccatggcccg acttgcaacg
    34141 ttcggagtgg ggtggatccc gtggggggac gaggtgtcgg acctcccggg ggcgatcgtc
    34201 cgcatgcgcg agggggtctc cgctgcggat cgggacccgt ccgagatcgg ggtgcagggc
    34261 cgggtgccgg tccggcgccg cgacgacggc tcggtcgacc tggacgccac catgaaggcg
    34321 gtcccggctc tggtggaggc gggcgtgacc gacgtgcgcc tcgacatcgc tgtccccgac
    34381 ggggccgacc cggtcgaaca cctgggtgag gtcgtcgagc gattccgtgc cgtcaccggt
    34441 cggagcgact gagccgcccg gtcggttccg ggtccggtcc ggatccggac cggctcaggc
    34501 ccgagcgtgc ttgagcgcct tggtggcgcg ggcccggtcg ccggcgtcga gctgcacctt
    34561 gcgcagccgg atcacctcgg gggtcacctc gacgcactcg tcctcggcca cgaactcgag
    34621 ggcctgctcg agcgacagga ccctcggcgg cgtgagccgc tcggtgttgt ccgagcctgc
    34681 ggcgcgcatg ttcgtctgct tcttctcccg gcagatgttg acgtccatgt cttcgctccg
    34741 ggagttctcg ccgacgacca tgccctcgta gacgtcgtcg ccgggggcga cgaacagcgt
    34801 tgcgcgctcc tggatggcga tgatcgcgta ggcggtgacg ggaccccgac ggtcggccac
    34861 caccgaacct cgatcccggg agcggatctc gccgaaccag ggcgcccagc cgtcgaagac
    34921 ctggtggatc atcccggtgc cgcgggtctc ggtcaggaac tcggtgcgga acccgaggag
    34981 tcctcgggcc ggcacccgct gctccatgcg aacccagccg gtgccgtggt tgacgatgtc
    35041 ggtcatctgc cccttgcggg tggccaggag ctgggtcacc gcgcccagga actcctcggg
    35101 cacgtcgacg gtcacggcct cgaccggctc gtggagggtg ccgtcgatct cgcgggtcac
    35161 cacctgcggc ttgccgacgg tcagctcgaa gccctcgcgt cgcatggtct ccaccaggac
    35221 cgccaactgg agctcgccgc gggcgtggac ctcccaggcg tcgggacgtt cggtgggcag
    35281 cacccgcaac gacacgttgc cgaccagctc gctgtcgagt cgccccttga ggaggcgggc
    35341 ggtcatcttg tcgccgtcga gaccggccat cggcgaggtg ttgacgccga tcgtcatggc
    35401 gaggctcggc tcgtcgacgg tgatgaccgg caacggtcgg gggtcgtcgg gatcggcgag
    35461 cgtctctccg atcgtgacgt cctccagacc ggcgaccgcg atgatctcac ccggacccgc
    35521 cgaggcagcc tcgacgcgct cgtggttctc ggtcacgtag agcaccgcca ccttggcccg
    35581 ctcgacggtg ccgtcggctc gacaccacgc gacgggctgg cccttgcgga tcgtgccgtt
    35641 gacgatccgg cacatggcca gacgccccac gaactggctg gcgtcgaggt tcgtcaccca
    35701 cgcctgcagc ggcgctgcgg ggtcatgggt cggcgccggc aggtggtcga cgagcagctc
    35761 gaacagcggg gtcaggtcct cggcgaggtc gtcgacggtg tccggcttgg tgatcgaggc
    35821 acggccggct cgggcgttgc agtagacgat cgggaagtcg agctggtgca cgtccgcgtc
    35881 gaggtcgagg aacagctcct cgacctcggc cacgacctcg tcgatgcggg cgtcggcccg
    35941 gtcgaccttg ttgaccgcca ggatcaccgg tagatccagg gtgagcgcct tgcgcagcac
    36001 gaagcgggtc tggggcaggg gaccctcgga cgcgtcgacg agcaggacga cgccgtcgac
    36061 catggtcagg gcgcgttcca cctcgccgcc gaagtcggcg tggccggggg tgtcgacgat
    36121 gttgatcttg acctcgtcgc gaccctcgcc ggtccagcgc accgcggtgt tcttggccag
    36181 gatcgtgatg cccttctcgc gctccaggtc catggagtcc atgacccggt ccacggtctc
    36241 ggccccctcg cggaaagcgc cggcctgctg gagcatggcg tcgaccaggg tggtcttgcc
    36301 gtggtcgacg tgggcgatga tggcgacgtt gcggatcccc ggcacgggga gggaggacgg
    36361 gtcggtcatg gcacttccgg tttcgggggg ctcaccagtc tggccgacga ccggcccgcg
    36421 ctccgaaccg gcggctcggt cgtcgggcac gtcgctcagc gacggtcgag ccggcggcgg
    36481 atcgtctcgc ggcgtcgccg cagctcgctg taggtgacct tctcggtcga gtcgtccacg
    36541 atcccgaagc tggcgcggat ggcggcctcg tcgacggcga agccgtcggc ggacacgccg
    36601 agctcgttgg cgaagcgctc gccgtgggtg gtggcgagac cggtcgacag cgacgccacc
    36661 tcggccacca ggtcgaggtc tggcgcgagc accgcgatgg cgccgtcgag gaacacgagg
    36721 ttcttgacgt agagcatgag gatcttgggg agtcgtgcgc ccatgcccat cagggccttg
    36781 atgatccgct ggatctcacc gacgagctgt tcctggtcga gcgtggtcgg gtcgacgggg
    36841 gccttgtcga gtccgagctc gcggatgacc tcgtcgatgt cggtgtcggc ggggagtgca
    36901 cccaggtcgc ggaaggcggc gacctggccg gggatgtcgc ccatggcgcc gtacagcatc
    36961 atgcggagga acgcggagcg ctgcagaggc gtcatccggg cggtgatgcc gaagtcgagc
    37021 agggcgatgc ggccgtcggg ggtaacgaac aggttcccgg cgtggaggtc gccgtggaag
    37081 atgccgtgca cgatgcaccc ctcggtgaag cccttcatgc cgatgcggat gatctcgtgg
    37141 gtgtcgatcc cggcggcctg catcgactcg aggtcctcga acgcgaaccc ctccaggcgc
    37201 tccatcacca gcacacggcg ggtgaccagc tccggatggg gccgagcgat gatccacccc
    37261 cgttggccga gctgggcgaa cacctcggcg acgtcgagca tgttctcggc ttcgagtcgg
    37321 aagtcgagct cctcgaggat ggtctcggcg aacagctcga ccagggccgg cgggttggcg
    37381 agggctgcga tggggatccg accgaccagg aacggcgcga gccacgacat gaccttgagg
    37441 tcggactgga cctgctggcg gatcgtcggc cgctggacct tgacgacgac ctcctcgccg
    37501 gtgcgcagcc tggccacgtg cacctgggcg atggaggcgg cggcgagcgg ctcgcgatcg
    37561 aaggacgtga agacgtcctc gagcgatcgg ccgaggtcct cctcgaccac ccggcggacg
    37621 acgtcgaacg gctcggctcg cacctggtcg cggcacttgc ggaactcctc gaccagttcg
    37681 ggagggaaca gcccctcccc ggaagagatg atctggccga gcttgatgta ggtggggccg
    37741 agtcgctcgg cggccagcct cagccgccgt gacaggccgg cccgtgaggt cggcccgccc
    37801 gcccgacggt ctcgcagggc ccagccgccg accgcggcgc cgaggcggac cgacacctcg
    37861 gtcaaccgca acggcggggg cagtcgccga cgaccggtga ggatcgggac ctgacgtcgg
    37921 accgtcatgc gcagcacggg caggccgcga gcccaggcga gcgagtcgcg gtcgagcacc
    37981 cacggtccgt tctcggtgaa ggtgaagcgc tcggtgtcgg gggagacggc ggcgtcggtc
    38041 atcgtgtggt tgggatggtt cgggcccggg tcattgtcgc cctgcggccg agagcgcggc
    38101 ctcagccaac tcgacgcggc agatcaggag gtcggggagg taggtgctcg gttggttgta
    38161 gcgcagagcg cttccgtcga gacgactgca gtgcagccca gccgccgatg cgacggcgac
    38221 cggggcgcag ctgtcccact cgtgctggcc accggagtgt gggtagagct cggcctcgcc
    38281 gcgcaccacg gccatcgcct tggcgcccgc ggagcccatc gggaggagtt cggcgccgag
    38341 cgcctcagcc atctgtcccg cttcgatcgg cgggcgggaa cgactcacga cgacgcgagg
    38401 cggctggtgc gcagctggta gcgacggtgg gtcgtcagtg gcgagcacca tgccgcgggc
    38461 aggtagggca acggctccgg cggtcggctc atgatcctcg accagggcga catggacggc
    38521 ccagtcggag cgcccctcgc tgtactcgcg agtcccgtcg agaggatcga tgatccaggt
    38581 gcgtgaggcg ccgaggcggc gtggatcctc ccgctcgctg cgggtcgact cctcggacag
    38641 cacagcgtcc tcgggccgga ggaccgtaag gcgttcgacg agaagtcggt gtgccgcggc
    38701 gtcgccccgt gcccccagct cccgggcatc cgcggcggtc gctgaccgct gcatgctcaa
    38761 cagcaggtcg ccggcctcgc gggcgagatc gtgggcgagt cgatgatcgt cctcggtcgc
    38821 cacggtcggg cacgctaccg gtcctccggg gcgtcgtggt cccgaccgat acttgacctg
    38881 aatggtcggg tattcggtac gcttcgccga ccgcccccgg caccgtcggg cgcaccggcc
    38941 caggaacgat cgtgatcacc tacgagctca ccaacctcca agccctcgag gccgagtcga
    39001 tccacatcat gcgcgaggtg gcggccgagc tggagcggcc agtgctgctg ttctccggtg
    39061 gcaaggactc gatcgtgatg ctccgactgg ccgagaaggc tttctggccg gccaggatcc
    39121 cgttcccgat gatgcacatc gacaccggcc acaacttcga cgaggtgtac gagttccggg
    39181 atcgacgggt ggccgagctc ggggtgaagc tcgtggtggc ctcggtccag gagtcgatcg
    39241 accagggccg ggcggtggag cagaccggac cgcgggccag ccgaaaccag ctgcagacgg
    39301 tgaccctgct cgacgcgatc gagctccacg gcttcgacgc cgcgttcgga ggggcccgtc
    39361 gcgacgagga gcgggcccgg gccaaggaac gggtcttctc gttccgtgac gagttcggcc
    39421 agtgggaccc gaagaaccag cgccccgagc tgtggaacct ctacaacggt cgacatcacc
    39481 ggggagagca catccgcgtc ttcccactga gcaactggac cgagctcgac atctggcagt
    39541 acatcgaggc cgaggggatc gaggtcccgt ctatctactt cgcccacgag cgtgaggtgt
    39601 tccagcgcga cggcatgtgg ttggcggtga ctccgttcgt gacgctgatg gacgacgagg
    39661 tgcccgagac ccgcacggtg cgctaccgga ccgtcggcga cgcgtcgtgc accggtgcgg
    39721 tcgagtcgac ggcgtcgacc gtcgcccagg tcatcgacga ggtggcatcg acccggatca
    39781 ccgaacgggg tgcgaccagg gccgacgacc gcgcctcgga agccgcgatg gaggaccgca
    39841 agcgggaggg ctacttctga tggagttgct tcgattcgcc acggccggat cggtcgacga
    39901 tggcaagtcg accctgatcg gccgtctgct ccacgacgcc aagtcgatct tcgaggacca
    39961 gctcgatgcc gtcaccgacg catcgcagcg gatgggtctc gacgccccca acctcgccct
    40021 gctgaccgac ggcctccggg ccgagcgcga gcaggggatc accatcgacg tggcgtaccg
    40081 ctacttcgcc accccccgcc ggaagttcat catcgccgac accccggggc acatccagta
    40141 cacccgcaac atggtcaccg gtgcgtcgac cgcggacctg gcagtggtgc tggtggacgc
    40201 gcgcaagggt ctgctcgagc agtcgcgtcg gcacgctttc atcgcgtcgc tgctgcgcat
    40261 cccgcacctg gtcgtcgcgg tcaacaagat ggacctcgtc gactacagcc aggcccgctt
    40321 cgacgagatc gtcgaggagt tccggcagtt cgccatgaag ctcgaggttg cagacctggc
    40381 gttcatcccg atctccgcgt tgcacggcga caacgtcgtg caacggtcgt tgaacatgga
    40441 ctggtacgag ggcgcatcgc tgttgcacca cctcgagagc gtgcacatcg catcggaccg
    40501 caacctcatc gacgcccgct tccccgtcca gtacgtcatc cggccccaga gcgatgagtt
    40561 ccacgactac cggggctacg ccggacaggt cgccggaggc gtgttcaagc cgggagacga
    40621 ggtcgtcgtc ctgccgtcgg gactgacatc tcacatcgcc gcgatcgaga cctacgacgg
    40681 tccggtggac gaggcgttcg ccccgatgtc ggtcaccatc cgcctgtccg acgacatcga
    40741 cgtgtcccgg ggcgacatga tctgccgggt caacaaccgg cccatcgtcg cccaggacgt
    40801 cgacgcgatg gtgtgctggc tgtccgacgc tgcggagctg accccccgca ccaagctggc
    40861 gatcaagcac acgacccgct ccgcccgtgc tctggtcaac gacctccagt accgcctcga
    40921 catcaacacc ctgcaccggg acgagagtgc cgagcggctc gccctgaacg agatcgggcg
    40981 ggtcaccatg cgggtgaccc agccgttgtt catcgatgag taccgccgca accgggtgac
    41041 cggctcgttc atcctcatgg acgaggccac cgacaccacc gtcggagcgg gcatgctgct
    41101 cgccggatcc tgaccgtgcc cgccggtgac ccgaccgcgg cggtgtcgcc cggtgtggtc
    41161 tggcacgacg gagcggtgtc gcgggacgag cgggcggcgg tcaccggcgg ccgggggatg
    41221 gtggtgtggt tcaccgggct gtccggttcg ggcaagtcca gcgtggccgt cgagctggag
    41281 cgtcagctga tcagctccgg tcgggccgcc tacgtcctcg acggtgacaa cctgcgccac
    41341 ggcctcaacg gcgacctcgg cttctcgtcc cgcgatcgcg gcgagaacgt ccggcgggtc
    41401 ggcgaggtgg cagctctgct cgccgattcc gggacggtcg ccctggttcc gctggtgtcg
    41461 ccgtaccgcg acgaccgcga ccgggtgaga cgtcggatca cctctgccgg gctcgacttc
    41521 ctcgaggtgt tcgtcgacac cccgatcgag atctgcgagg agcgggaccc caaggggctc
    41581 tacgcccgtg cccgggcggg tgagctcacc gggatgaccg gggtggacga cccctacgag
    41641 gcgccgtcga gaccggagct ggtgctgcgt ccctcggacg gcgatgcggt cgccatggcg
    41701 gagatcgtcc gggcgcggtt gcggtcctga cgctgcgccg tggccgatcg actcacgcgg
    41761 tctttgcctc cgcggcgcgg gctcggttgc ggaccaccgg ggtggtgtcg accggcccgt
    41821 ggggttctcc ggccaggtcg tagcagtcgg cgtggaagtg ctcgacccga tcccacacgc
    41881 ccttgatgta gacgttgcag atgacctggc ggttccggac cttggcctgg aacttgaccc
    41941 gttcgtcgca gtgggcgcac tcgacggtgc tgcccggttc gacgtcgcgg agcacggccc
    42001 ggctgcgccg gggagcggtt cgttgggacg gtcggtcgac cgtagaggtg ctggaggagg
    42061 ctgccatgcc ctcaggtatc gggtgacgcg tgacaccgcc tgagccctgg gagcgattgg
    42121 ggccgaacga cccaggcggc gcccgaggat gggccggagg gcccatggac gccggcgtcg
    42181 gagaggatca ggctcgcggg ccgcggcgtg ccggtcgcac cgcccgggcc cgtcgcccca
    42241 gttgcacggc gtcgcggacc agctcgcgca cctgggcctc gtcggcgccc tcggccacca
    42301 gggtctggat ctgtgcctcc atggcctggg cgcgggcttc gtcgaaggcc acggaatcgt
    42361 cgcgggtggt caccgaggtg acgaggatga ggcacactgc ggccaccgcg gtgacgagcc
    42421 cgaaggtgat ggcggttccg ttgttgtcgg cgatcgagcc gacgatcatg ccggcgattc
    42481 caccgacgaa gaccacgagc acgagtcgtc ggacgatcct ggggtccacc gtttgtcgcc
    42541 taccgcgtca ggggcttgta cttgatgcgg tgcggctggg aggcgtcggc tcccagctcg
    42601 cggcgacgga actcctcgta ctcgctgaag ttgccctcga agaaccgcac ctgcgagtcg
    42661 ccttcgaagg ccaggatgtg cgtcgcgatc cggtcgagga accaacggtc gtgcgagatg
    42721 accaccgcgc agcccgggaa gctgtcgagc gcctcctcga gcgcccggag cgtgtcgacg
    42781 tcgaggtcgt tggtcggctc gtcgagcagg agggtgttgc cgcctcggcg caggagcttg
    42841 gcgaggtgta cccggttgcg ctccccgccg gagagctcgc cgaccggctt ctgctggtcc
    42901 gagcccttga agttgaagct ggcgcagtag gcccgaccgt tgacctcgcg ggtgccgacg
    42961 gtgagcacgt cctgtccgtc ggtgatctcc tcgtagacgg tgtgggcgtc gtccaggctg
    43021 tcgcgggact gatcgacgta ggccagctcg acggtcgaac cgacgaccag ggtcccgtcg
    43081 tcgggacgca gctcggggtc gccggctgag gtcgcggcgt cgacgatcat gcggaacagc
    43141 gtggtcttgc ccgcgccgtt cgggccgatc accccgacga tgcccgcccg cgggagcgag
    43201 aacgacaggt cttcgatcag cagccggtcc ccgaagccct tgcggagcgc gctggcctcg
    43261 atcacctggt ctccgagccg cggtccggga gggatcgcga tctcgagccg ggagctggtg
    43321 tcgcgggcct cctcggcttc cttctgcagc tcctcgtagg ccgacagccg ggccttggac
    43381 ttggcctgtc gggccttggg tgccatgcgg acccagtcga gctcacgctc gagcgtgcgt
    43441 ctgcgggctt cgttctgctt ctcctggcgg ccgaggcgct cctgcttctg ctcgagccag
    43501 ccggagtagt tgccctcgaa cggatgaccc ttgccgtggt cgagctcgag gatccagcgc
    43561 gccacgttgt cgaggaagta gcggtcgtgg gtgatggcga ccaccgttcc ggggtagtcc
    43621 tggaggaagc gctcgagcca ggccaccgac tcggcgtcga ggtggttggt cggctcgtcg
    43681 agcagcagca ggtcgggctt ggacagcagg agccgacaca gcgccacccg tcggcgctca
    43741 ccgcccgaca gcgtggagac atccgcgtcg ccggggggca ggcggagcgc gtccatcgcg
    43801 atgtcgatcg tgcgttgcag gtcccagccc ccggccgcct cgatgcgggc ctcgagctcg
    43861 gcctgggcgt tgcccagctt ctcgtagtcg gcgtcggggt cgctccaacg cgccatgagc
    43921 tcctcgtagg cggtgaggag cccggccacc tcggcgacgc cgtccatgac gttgccgacc
    43981 acgtccttgc tctcgtccag ccgtggttcc tgctccagca atccgaccgt gaaccccggg
    44041 gtcaggcgcg cctcgccggt gtggtcgtcg tcgacgccgg ccatgatgcg cagcagcgat
    44101 gacttgccgg acccgttggc cccgatgacg ccgatcttgg cgcccgggta gaacgacagc
    44161 gagatgtcct tgaggacctc tcggtccggc gggacgaact tcccgacgcg gtgcatggtg
    44221 aagatgaact gggcgcccat gggtcgggac tgtaccggcg ggccggacgc ggtccggaac
    44281 cggtctgcgc ccggaggtgc cggccggccc gggtcggccc ggtcagacga gccggtggtc
    44341 gagcggatgg ccgagcgggt cgtcgagccg acgaggaacg tcgcacgcat ccccccggac
    44401 gcgccggtgc ggcccgatcg ctcgggccgc accggtctcc gatgtcgtcg gctcagcgcc
    44461 tggcgggtgc cttcttggcg ggtgccttct tcttggccgg ggccttcttg gcgggtgcct
    44521 tcttcttggc tggcgccttc ttggcgggtg ccttcttctt ggcaggcgcc ttcttcgccg
    44581 gcgccttct
//
LOCUS       DAOQOD010000005        42798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_271_length_42798_cov_11.455560, whole genome shotgun sequence.
ACCESSION   DAOQOD010000005 DAOQOD010000000
VERSION     DAOQOD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 42798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42798
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_42798_cov_11.455560"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1073
                     /locus_tag="PLZ72_01335"
     CDS             123..1073
                     /locus_tag="PLZ72_01335"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNA34966.1"
                     /translation="MEKGRSELTLRAYRQDLRRYAGFLARRGLDVDDVTEADVQAFVT
                     ELRSGELAAASVIRIAGTVRAWHRFRAAEGDSPSDPTSAVEVPRRPSALPRALSEADV
                     GSLLDAVDSACRMPSGDAVADALALRDRALLELLYSSGIRVSEACGLGFGDLDVDSAF
                     ARVLGKRSKERLVPIGRPALAALADWLDRGRPVLLGSRAVDRDAADAIFLGQRGRRLG
                     RQAAWEAIRRWAAAAGLRGEISPHVLRHSCATHLLDHGADIRTVQELLGHASVSTTQI
                     YTRVATERLVQAYLDAHPRARVAGSASRVGERSNGGPVTR"
     gene            1070..1627
                     /locus_tag="PLZ72_01340"
     CDS             1070..1627
                     /locus_tag="PLZ72_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA34967.1"
                     /translation="MNAPELQPTHRSRRFFASLSGAAPSEEDAAWAEGWLSDHERAAF
                     RKMPPADRRHSVAVARAVVAHLDRLGLTEADPDARWIVVAALMHDVGKSVTGLGTYGR
                     VVATLSGWVGGHDMAASWADTRGFTRRVGLYLQYPELGADVLRMAGSDDRVVAWAAEH
                     HHPEEDWTVPVEAGRLLQLADDGRL"
     gene            complement(1684..2685)
                     /gene="trpS"
                     /locus_tag="PLZ72_01345"
     CDS             complement(1684..2685)
                     /gene="trpS"
                     /locus_tag="PLZ72_01345"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNA34968.1"
                     /translation="MPRVFSGIQPTGDLHLGNLLGAVRNWVQDQHDADSFYCIVDLHA
                     LTVPKAPGEVGAFSLEMAQTLLACGLDPDVCTLFVQSHVSEHAELAWLVQCVTAMGEL
                     NRMTQFKDKSARQGGKQDLVSVGLYTYPALQAADILLYDTDRVPVGEDQRQHIELTRD
                     VAERFNARYGETFVLPEATIPRAGARVMDLQEPTDKMSKSATSDAGVVYLMDDLAVTA
                     KKFKRAVTDSDGTVAFDPVAKPGVSNLLSILGAATGRSPEDLADEYSQYGPLKVDTAD
                     AVVALLEPIQARFRELVEDPAATNELLAIGAEKARAVAADVYDRAVRNVGLLPRPVL"
     gene            2921..3700
                     /locus_tag="PLZ72_01350"
     CDS             2921..3700
                     /locus_tag="PLZ72_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNA34969.1"
                     /translation="MGYAVSTPVFEGPFDLLLHLILREQVDLYDISLSTIVDAYLAEL
                     NRMEHLDLEVATEFLLIAATLVELKTKRLLPGDDDIDVDEDLSLWEERDLLLARLLEC
                     KTFKDAAGTLAHLHATAARSVPRTVGPDDRFWDLTPDMLEGVTPEQLFAAYQKAITPR
                     PTPVIDLFHVAPIRTSVIDTVADLCVELPRRGAVSFRELTSSLVDRLDVVVHFLAVLE
                     LFKQGLVDLHQPQTFGDIRISWIAGDSDALGDLALVDAYDG"
     gene            3702..4532
                     /gene="scpB"
                     /locus_tag="PLZ72_01355"
     CDS             3702..4532
                     /gene="scpB"
                     /locus_tag="PLZ72_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNA34970.1"
                     /translation="MPDGAGPADDPAETEAASPDGPDDLSPIGDEARRALEAVIMVAD
                     QPIEASILAQLVERSPQQVEDLLAGLVASYEEDGRGFQLVRVAGGWRYQSHEDLSPYV
                     ERFVLSGQSARLSAAALETLAIVAYKQPISRAQVSAIRGVNVDGVMRTLQQRGYIAEV
                     GKDPTPGNPSMFATTPLFLEKLGLNDVDELPSLGDFIPGADVVELLEQGLRAEADTDP
                     TPQGAPGDAPEGPDPAATVEVVDAADAVDAAAEVIDVTDHAPIDVDDLIGDGDPLAEG
                     "
     gene            4529..5335
                     /locus_tag="PLZ72_01360"
     CDS             4529..5335
                     /locus_tag="PLZ72_01360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNA34971.1"
                     /translation="MNQNPTPGFVPADEGERLQKVLARAGFGSRRVCEDIIDAGRVTV
                     DGRIAVLGARVRVGDQEVAVDGTPIGIAPDLVHYLLNKPAGVVTTASDTHGRSTVIDM
                     VPDQPRVHPVGRLDLDTEGLLILTNDGVLTHRLTHPSFGVEKEYVAEVEGRPARGALR
                     RLRDGVELDDGMTAPARVSEIQPGVLRLVIHEGRNRQVRRMCEAVGHPVRRLVRTRIG
                     PLRDPQLGPGNWRELTRDEVRALERAAAPLGPDGERSAGATGSGAPSGPG"
     gene            5345..5716
                     /gene="aroH"
                     /locus_tag="PLZ72_01365"
     CDS             5345..5716
                     /gene="aroH"
                     /locus_tag="PLZ72_01365"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNA34972.1"
                     /translation="MPTTSVLALRGATTLDADERDHLLERVRELLTQMFRTNDVDHDD
                     LISILFTATPDVHSAFPALAARQMGLGDVPLMCAQELDIDGAKPRCIRVMAHINSTRT
                     RSELHHVYLHDARDLRDDLPR"
     gene            5724..6851
                     /locus_tag="PLZ72_01370"
     CDS             5724..6851
                     /locus_tag="PLZ72_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNA34973.1"
                     /translation="MTAPAPDAGSAEPAAAAADDTGVPMRRRAGLVGTGLIGSSIGKA
                     LRERGWHVTGSDREPARATAALDAGAIDAIGHDPAAEITFVAVPVGAVADSARPALAD
                     GGVVTDVGSVKSPVAAALPHSRFVGGHPMAGSEAQGVAGSRSDMFEGATWVLTPTEAT
                     DPAAQALVHSVVRSLGAEVVTLSPEDHDRLVATVSHVPHLTAVTLMGLASQRAEHDAA
                     LLRLAAGGFRDMTRIAAGDADIWIDICDDNRAAILDVLDDLIARLGTMRDVVDRGDGP
                     SLGEQLLAAQEARRNLPTGAPPAEELSEVRVAIPDQPGELALVTTLATELGVNVYDIE
                     VVHAAGERRGMLVLVVSTSRAEALVDALRGRGRVASTQELT"
     gene            6874..8217
                     /gene="aroA"
                     /locus_tag="PLZ72_01375"
     CDS             6874..8217
                     /gene="aroA"
                     /locus_tag="PLZ72_01375"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNA34974.1"
                     /translation="MTADPARSSSAGADEVREIVPVPGPLDAVVRPPGSKSVTNRALV
                     AAALARGTSNLVGTLDADDTAAMLDCLRGLGIPIGDGSDGSNGSIRVVGCGGRPPLDG
                     AILDARLSGTTSRFIAPVAALARGTVVLDGSDPLRRRPMAALFDALEALGAGIEPLGE
                     PGHLPVRITGSPRDVLGGSVQVTGNVSSQFLSALLLSGPCFDDGLTVGVTTDLVSVPY
                     VQLTLDVMAAFGAAVDHDDGWRTITVSPGGYRAVERFDIEPDASAASYWAAAAAIAGG
                     SVRIDGLGRSSRQGDVAFIDVLASMGAEVTWSDHDVVVRGVGTLRGVTVDMADISDTA
                     QTLAAVAVFAEGQTTVTGIGFIRGKETDRIAAVVSELRRLGIDASEDPDGFTVRPGPV
                     RPTDVHTYDDHRMAMSFALVGMRAPGVRILDPGCVAKTYPGFWDDLARVSGTASL"
     gene            8229..8906
                     /gene="cmk"
                     /locus_tag="PLZ72_01380"
     CDS             8229..8906
                     /gene="cmk"
                     /locus_tag="PLZ72_01380"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNA34975.1"
                     /translation="MEPSDVPVIAIDGPAGSGKSTVARRLADRLDLKYLDTGAMYRAV
                     AFAAIRQGLDPVDVEPVAALATHVDLVVGDDAVVVDGVDATVEIRGPEVSRAVSVVAA
                     NPAVREEMRTRQRVWVVENRGGVVEGRDIGTVVFPDAVLKVYLTADPAVRAARRAKEM
                     TDLEYDTVAADIARRDAADQGRSDSPLVEADDAVTVDTTGLGIDEVVEQIASMVESRL
                     ADRDATG"
     gene            8944..9615
                     /locus_tag="PLZ72_01385"
     CDS             8944..9615
                     /locus_tag="PLZ72_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA34976.1"
                     /translation="MSRVVVRRGALRPISTGGRLSYRIIRAIGRVLIAPLWRVSVEGR
                     ERLPVGVAYVVAPVHRSYVDFAVLVAAMPREMRFMTKDSVWKWRWLGRFIEFNGSFPV
                     DRAHTDRDALRRCEEAIGLGDPVVMFPEGRRKDGPLVEDLFDGPAFVACRTRVPVVPV
                     AIGGSDRAMPVGARMIRPAKVRLVIGEPIYPDVPAAGRIPLRAVGENTEALRTELQRL
                     YDQVR"
     gene            complement(9709..10101)
                     /locus_tag="PLZ72_01390"
     CDS             complement(9709..10101)
                     /locus_tag="PLZ72_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34977.1"
                     /translation="MTAFRLFLHILAASVWVGGQIVMAGVLPTTRRLEPESRVAVATA
                     FGRVAWIGLAVAVLTGLWNVMAIPMDELPHPWVEVHLLAVLVTAAGAFLHTIARGRPA
                     LLAVGGTTAALGAIAAMYLGVLVTINVA"
     gene            complement(10190..11257)
                     /gene="ispH"
                     /locus_tag="PLZ72_01395"
     CDS             complement(10190..11257)
                     /gene="ispH"
                     /locus_tag="PLZ72_01395"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNA34978.1"
                     /translation="MSDDSNATVERVLLAAPRGFCAGVEMAIKALAWMVRAFDGPVYC
                     YHEIVHNQQVVQRFEEQGVVFVDDVAEVPPGRPLMLSAHGSAPEVVQAARDNGGFVVD
                     AVCPLVTKVHHEVKVRANKGYQIVYIGHEGHEEAVGTMAVAPEAIHRVESVEEVDALP
                     DFDTPVALLAQTTLSHRDWADVASATAERFPDVWTPGRSDLCFATTNRQSALMEIAPR
                     CDAMVVIGSANSSNTVALERLARGAGCATVLRINTADELPRRADGSVDLHGVVGVTAG
                     ASAPEELVEQVIAALAPRDGVEEVHLTDEDEYFPPPRNLRDMLVAIDALGALGLGADP
                     TVRVASDDRNRAASEVLADLT"
     gene            11321..12784
                     /locus_tag="PLZ72_01400"
     CDS             11321..12784
                     /locus_tag="PLZ72_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNA34979.1"
                     /translation="MSLATVLSVAPGSPAAESGVVPGDELVSIGGRPLRDVIEFRVLS
                     DEADVELELRRGGLELNVEIAKAHGEPLGLEVSSPVFDEVRTCDNHCEFCFIYQLPPG
                     MRRSLHLKDDDYRLSFLYGNFTTLTRFTEADLERVLVEGLSPLYVSIHATDPERRVEM
                     LRNRRGAMSLRWLRALLDHGVEVHGQIVVCPGLNDGDVLDETLCGVLDEYPELADVAV
                     VPLGVSRFNKEPRMRPHTVAEAERVCDVVDRWQEVFLSTVGHRMVFAADEYYLLAGRP
                     FPDGATYGDFSMHEDGIGMARAFEAELDGTSEVGIGIQSGFFHSVDGAPADGYRAPRT
                     EPTPAPAPVALGPRRRSPEPEPEPAVGPAVEPEGRVAVLTGPYGARILTPLIEDLLRR
                     EGRGGPDDAGVRVVEVENRFFGGNVGVAGLMVGEDLARVLAAQPVGDRYLLPDVCLSR
                     GVFLDGTRPEDLPRPVEVVPTDGVALRRVLLEGVNRG"
     gene            12777..14090
                     /gene="der"
                     /locus_tag="PLZ72_01405"
     CDS             12777..14090
                     /gene="der"
                     /locus_tag="PLZ72_01405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNA34980.1"
                     /translation="MAELPIVAIVGRPNVGKSTLMNRILGQRVAIVEEKPGVTRDRKE
                     VEAEWQGRPFLLVDTGGWMVGGSDLDRKVSNQSEQAIKDADVVLLVVDAKVGVTGEDA
                     QVAQLLRALDVPVHVLANKVDDTSHEALIWELMSLGLGEPLPISALHGRGTGDLLDVI
                     IAELPEVPDDDVEQSDMDEGEGVISVALVGRPNVGKSTLFNRLIGEDRAVVHDMPGTT
                     RDAVDTVVDTEIGPLRFVDTAGMRRKSRIDEGTEYFSMVRALKAVDESDVALLVIDAT
                     EGVTHQDQRLAERVDGAGCPIVVLLNKWELLDEDRRKDVLYQLGQRLHFLGDSAVLRI
                     SALTGRGVHRLLPALEDSIDAYHTRIPTRQVNDVIRRAQQAQPGPHGARVLYATQGAA
                     DPPTFTLFANKTLPASYLRYLERQLREAFGLEATPIKMRVRRRGG"
     gene            14236..14631
                     /locus_tag="PLZ72_01410"
     CDS             14236..14631
                     /locus_tag="PLZ72_01410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNA34981.1"
                     /translation="MSQVRDEIEARGKPQLGAVDAVTELVVTDEVEGQGDEVTAGATV
                     TAHYVGVSASTGAQFDASWDRGAPISFPLDGVIRGWSEGLLGMKVGGRRNLVIPGDMA
                     YGANPPPGAGIAPNETLVFTVDLVDVSWR"
     gene            14622..15227
                     /locus_tag="PLZ72_01415"
     CDS             14622..15227
                     /locus_tag="PLZ72_01415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNA34982.1"
                     /translation="MALSPGRSRRRRGVALLAAGAVALGGLAVACGDSESATTSTTIE
                     SKGAAGTSGSGSSSGAGSSDTSAPAGANSEFAAEIEARGAPTLAAVSGPVTELKVTDD
                     VAGTGTAAEAGSTVTVQYVGAVADTGKVFDESWSSGQPVTFPLDRVIPGWSQGLVGMK
                     EGGRRTLVIPAELAYGANPPPGSGIPADAALVFTVDLVKVD"
     gene            15229..16821
                     /locus_tag="PLZ72_01420"
     CDS             15229..16821
                     /locus_tag="PLZ72_01420"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNA34983.1"
                     /translation="MAEPGDESLAGDGSERPSGPVARFASASARARAGNLERGGEKLA
                     AQGKLFVRDRIDLLCDPGTFVEDQLLANSPYRDDMSPDLPADGVVTGVGEIDGRPVAV
                     MANDPTVKAGSWGARTVEKIVRLTETALRDEIPVVWLVDSAGARITDQVELFPGRRGA
                     GRIFYNQVRLSGKVPQVCCLFGPSAAGGAYIPSFCDLVVMVEGNASMYLGSPRMAEMV
                     VGEVVTLEEMGGARMHATVSGCGDNLAVDDEDAIEQARAYLTYLPSRWSEDPPAYHPV
                     EPSRPLTAEIVPEEEAQGYDVHDVLDGLLDAESFFEIKPLFAPELVVGFGLLEGRPVG
                     IVANNPMHKGGVLFTDSADKAARFIWLADAFGVPMIFLADVPGFMIGSQVEREGIIRH
                     GAKMITAVSEATVPKVSVIVRKAYGAGLYAMSGPAFEPVATIALPTAKIAVMGPEAAV
                     NAVFANKIAAIEDPDEREAFVAEQREIYETDVDLLRLASELVIDAVVEWEDLRSDVSR
                     RLRRAAGKDRTFSVRRHGVPPV"
     gene            16855..17094
                     /locus_tag="PLZ72_01425"
     CDS             16855..17094
                     /locus_tag="PLZ72_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34984.1"
                     /translation="MPWCEECSRFYTPSTLTPEGDCPSGHHVADPDTAIHQSAASPRD
                     DEHSAKVPWHFWLLLAAVVIYLGWRLIQGVQWLLG"
     gene            17264..17338
                     /locus_tag="PLZ72_01430"
     tRNA            17264..17338
                     /locus_tag="PLZ72_01430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17299..17301,aa:Pro,seq:ggg)
     gene            17513..17803
                     /locus_tag="PLZ72_01435"
     CDS             17513..17803
                     /locus_tag="PLZ72_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNA34985.1"
                     /translation="MGTLRAVVEAKRREISDLVRHHRGRSVALFGSVARGDDTPESDI
                     DFLVEFEPGSSLFDLMDLQEALGQLLGVPVDVVSVGGLKDRDDHIRREAVPI"
     gene            17800..18129
                     /locus_tag="PLZ72_01440"
     CDS             17800..18129
                     /locus_tag="PLZ72_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNA34986.1"
                     /translation="MSRADDLRIADVREAAQQLATLVADGREAFDTDWMRQRATERLL
                     EIIGEASNAVSDEFKSDHPDVPWRHVVNLRHLLAHHYHRVDSDQIWTIATVSVPALAR
                     ELQSTDE"
     gene            complement(18380..19459)
                     /locus_tag="PLZ72_01445"
     CDS             complement(18380..19459)
                     /locus_tag="PLZ72_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34987.1"
                     /translation="MWAAFLAALVAAGITACGSGGTTAEPSQTATGVVGSWSGPSVAN
                     APTPQATAVLLAGARPAVIEAVGSDVRVLEPDGDRLTARASEDLGAPVVWMQPRVDGD
                     ALVVLAQTCATPVSTVDSVDRDCPSAHTALISFTGDALEPSSAEVHVDGVPRDFASLG
                     DTAAVTTSTGFFVRDPDGTWTLTSEVIGPTCATDEAYVEHLIAASAPTTVADPGTSSG
                     GSFRVRPLKGAATDQRVPGRVDAITCAPDGYIASVSSGSAAADGVSLWGSTGVGAPEQ
                     KDGPSGARSTIWGQLSRGQWATATVGSRRTVFIDPTSGDWFAATGLKGSESPPYATRS
                     GDRGLVFGPAPGGGTWMLRAYRLDR"
     gene            complement(19501..20073)
                     /locus_tag="PLZ72_01450"
     CDS             complement(19501..20073)
                     /locus_tag="PLZ72_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA34988.1"
                     /translation="MPQPTEDRPAQIEVRRASVADAELVGGLYLRSWLAGYEGLVDPD
                     QLAPVAAERAAYDWPVAIEDPHARFAIGYVDAQPAGVAKVGPDPTENVRGVWLELLYV
                     TPEFWGTGVAAALLGWATGYAGTTGASAMRLRVVEAQARARRFYQREGWEPDPTSRPR
                     TTTSSRCCACAAASAEGQPCRPPDHLWSDG"
     gene            20159..20677
                     /locus_tag="PLZ72_01455"
     CDS             20159..20677
                     /locus_tag="PLZ72_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34989.1"
                     /translation="MEDRRPGSGRYARLEREQRWVLDAVPTEARPRAEIVDRYLLGTR
                     LRLRRMTSSDGLVLKLGQKVRVEATDPERVMVTSLYLSSEEFDVLASLPAAEIAKSRF
                     VLPWGTGALAVDRFHGRLRGLVLAEIEHGPDDELLNPPPFARADVTHDDRYSGGELAS
                     ATASEIAALLRR"
     gene            20744..21949
                     /locus_tag="PLZ72_01460"
     CDS             20744..21949
                     /locus_tag="PLZ72_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34990.1"
                     /translation="MAFDVFKDETFNFKVQAMLGELRTGCGDAGEIMSTASSITDGDA
                     DSWVAAWRALAERVEKIASDADAGGHRVSAREAYLRASAYHSAALEVVDGTSDPNAVL
                     KPTFAAHRRCFDRYVELSEPPIEKLDIAYEDTTMPGWFVPAAGSGRRPTIVLSNGSDA
                     PVISTLGIALAARARGYHSIMFDGPGQQSMLFERNIPFRPDWEAVITPIVDLLVARGD
                     VDDKKLCIYGLSQSGYWVTRAIAYEPRFAAAVVDPGVDDVSASWMGHLPEEMKEMLDK
                     GDRSTFDQWMQIGEQDQTPAEKQEIEWRAKPYGIEDAFDLFTAVRTYRLTPEDLARIT
                     IPLLITEPEGEQFWPGQSERMHQHVASSELVHFTAAEGADRHCEPMARSLLEQRMFDW
                     LDRTLNPSA"
     gene            complement(22026..22481)
                     /locus_tag="PLZ72_01465"
     CDS             complement(22026..22481)
                     /locus_tag="PLZ72_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HNA34991.1"
                     /translation="MDLDPSTIDLYVRHAFAGMNRVLDRLDDDTVNRTPDGWGTNSVA
                     GLIVHCCELAPSWFEMPGLGRDSVRDREAEFAATGTVTELRSRIDAAVARTSTLIGEF
                     AVGPTTPEHDFRAFMPGEDRSDGALVLHVLEELFQHLGHMEVTADALGR"
     gene            22684..23913
                     /locus_tag="PLZ72_01470"
     CDS             22684..23913
                     /locus_tag="PLZ72_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34992.1"
                     /translation="MVALAVACAPPTVPPPVVTNEWPGTVTQGRTIGTTGTVWGGLDH
                     NDDWLVRSRAGVDGTLTAAETVLTPRTGPGVGDLGAPVVLSPTYYSHPLYQSPLVSRP
                     YLGTHLLLAGGDGVTAANRIFVEQGGTWTAGGTYALNPNREVVVAFSDSYLVTRGSAA
                     SADLLQVHPIDVTGGVVTVGPAVSVSLPASWSDYLRIDGELAGDTLVVAGSDPIRPGA
                     ETAVAVADLSDPTVTALTQVWTGGDLVLTSFDLDVDGSVTRAAVGVEASQGSGTVGRV
                     MILSPTGSGPWATTAVLTPPAGLPDIGHGTYFGRQVALDDGLLVTLARFVARPSPAST
                     GTSNVGVLGAYRLVGGVWQFEASMTPAPDVITTPETSRLPAALDVAGTVVHVEISGSD
                     EGAPPAPTFAESWMFGS"
     gene            complement(23930..24910)
                     /locus_tag="PLZ72_01475"
     CDS             complement(23930..24910)
                     /locus_tag="PLZ72_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HNA34993.1"
                     /translation="MTRPTILLTDRGWDDADIERQLCDDAGYDLVDVQEPADEDALVR
                     AVTEADPVGILFCWAPVTARVLAAAPRLAVASRLGVGLDNIDLPEAARRNLTVTRVPD
                     YCVEEVSDHVVALVHNWARGVTAFDRDVRDGRWRPGMRDLRRVRDLTVGVWGLGAIGS
                     ATAAKFAALGCTVVGHNRSVVDPPAGVEVVSPADLLARSDVVSLHVPAVAGAGPLIGA
                     TELAAMRPGSLLVNTSRGALVDTDALVSALRTGTPGAAALDVLPAEPEVPEVLRAAMT
                     GPDAVDVTITPHVAFSSTASVTELRVRATEDLLRVLRGEPPRDPVPLPSP"
     gene            complement(24907..25869)
                     /locus_tag="PLZ72_01480"
     CDS             complement(24907..25869)
                     /locus_tag="PLZ72_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNA34994.1"
                     /translation="MRLARIATHDGAHLAVVEGTLSDDPALDGLTVVDITAVEATFGD
                     DVVAVLAAGDAGRDAVTAALAAAPASARRPLAEARLLAPVPRPPKFLAIGFNFRGHEA
                     DAGAAPPAFPLFFNKQTTCVTGPTDPIQAPPSSTQLDYEGELVMVIGRRCRHVPADRA
                     AEVVGGYLVGNDVSARDWQRRSPTVTLGKSFDATAPTGPWVTTADDVEDPHDLRIRTW
                     VNGEQVQDGRTADMITDCWAQIALLSSVFTLEPGDLISTGTPAGAAQQSADPRWLRPG
                     DRVSVAVDGLGLIDNEVVAAPETTVIDPHRRWPPSGPTAQGGSR"
     gene            complement(25866..27143)
                     /locus_tag="PLZ72_01485"
     CDS             complement(25866..27143)
                     /locus_tag="PLZ72_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNA34995.1"
                     /translation="MNDATITAVTGHRRWDSRGRPTVEAVVTTTSGTGRALAPAGAST
                     GSGEALDLRDGGDRFDGLDVTTAVSNISERIAPALIGLDAADQTTVDAALESLDPSVG
                     FSELGGNAVVAVSLANAHAAAASRGEPLWRTVLGPGVEVAGTVALPVPEIQIFGGGAH
                     AHRRIDIQDLMVVPRGATSFAQALDWTAEIYRAAGRWMDDRGLRMGVADEGGWWPALD
                     TNQQALDALTAAIEAAGLEPVTQVGIALDIAASQFGRNGRYRLGREERELDTAAMIDL
                     LVSWVDRYPIVSVEDPVAEDDPAGMAAFTAATGERIQVVGDDFLVTDADRVARAAEDR
                     SANTVLIKPNQAGTLTRARAALDAAHARGLQTIVSARSGETEDVSVAHLAVGWGSNGV
                     KVGSITRGERTAKWNEMLRIEESLGDRARGLRR"
     gene            complement(27242..28258)
                     /locus_tag="PLZ72_01490"
     CDS             complement(27242..28258)
                     /locus_tag="PLZ72_01490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNA34996.1"
                     /translation="MTAGTELPAAIRRGLNALGLLAEGDGVSGVPLTGGVSSDIWRVD
                     IATGPVVAKRALERLRVEATWIVPTSRTAHEAAWLRAAARAVPALVPRALGYDAASGV
                     LVLEWLDPARFGNWKAQLAAGPLTDDVGAGVAAALGDRLGAVHAAMSDPVRDAAAFAA
                     GDLFGALRLDPYLRVTAGAHPDMAGRLDDLAERTSATGRTVIHGDVSPKNVLVGPDGP
                     VLVDAECATWGDPAFDVAFCLNHLLLKGLWHPDDAAVLARAADALVAGYRSHVAWEDP
                     DKLDERVATLVPALALARVDGASPVEYLDDAQRDEVRHRARTLLVDTPATVADLLTRW
                     FHSP"
     gene            complement(28255..29370)
                     /locus_tag="PLZ72_01495"
     CDS             complement(28255..29370)
                     /locus_tag="PLZ72_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA34997.1"
                     /translation="MSVERTFVGLPSPGFGRFGAGGHPCQGVYHTPAGVRPEVAFVAS
                     HYNIDFSEHYLADLLAGHGFGFLGWNTRFRGDEAHFLLDHALADVGVGVRWLRDQAGV
                     SRVVLLGNSGGGSLMAAYQSQAVDPNVTPLPGMRPAAGIDDLIAADAYVSLAAHPGRP
                     DVLTAWMDAAVTDETDPTATDPTLDLWNPQNGPPFSAEFVARYRAAQEARNQRITDWC
                     RDELDRLAAAGIGDRLFTTSRLWADPRTVDPTLEPTARRANRCYLGDPRRANYGTWGV
                     GQCSSLRTWLSMWSLETSQCRAEPHLARITVPSLVINADADTGVFPSDARTILDAIGT
                     ADKQLVEVAGDHYLIEPDDARPRTAALIADWVAGALG"
     gene            complement(29367..29888)
                     /locus_tag="PLZ72_01500"
     CDS             complement(29367..29888)
                     /locus_tag="PLZ72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA34998.1"
                     /translation="MSSASLHHVAFACKDPEATRHFYEDLMGFPLVHTEVAPVEKGFI
                     RHFFFALDDGSCIAFFDLHDAGEKPGWSTEIATGLGLPLWCNHLALRADETKQAEVRD
                     RVEADDSDVLMVLDHDWCESHYYLDPNGILIELCRDTPGMPTDAARARELLTAVPEAD
                     TPQAESRLVDHSR"
     gene            complement(30016..30612)
                     /locus_tag="PLZ72_01505"
     CDS             complement(30016..30612)
                     /locus_tag="PLZ72_01505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA34999.1"
                     /translation="MSENRWLQMLEANPGHSDWYIERFKAMAAEGRDLDGEARLIDAM
                     AGRGARILDAGCGPGRVGGRLAVLGHTVVGVDLDPALIDEARSVHPGATWRVGDLSEL
                     DLGDLDPFDLIVNAGNVMTFLDPATRREVLRRMGAHLAADGRLVVGFGAGRDYDFDEF
                     FADVDAVGLAVELRLATWDLRPFGPGSDFLVAVLVRGS"
     gene            complement(30660..31328)
                     /gene="nth"
                     /locus_tag="PLZ72_01510"
     CDS             complement(30660..31328)
                     /gene="nth"
                     /locus_tag="PLZ72_01510"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNA35000.1"
                     /translation="MKRQDKADRIGEILDDLYPDPPIPLDHTDPYTLLVAVALSAQTT
                     DKKVNEVTPALFAEASTPEDMVALGPDRILELIREIGLAPTKARNLWLAADRIVENGG
                     QVIEDWDFLESLPGVGHKTASVVMSQAFGVPAFPVDTHIHRLAGRWGLSSATNVERTE
                     RDLKAVFPIDTWNRRHLQIIYFGREHCPARHHDLTTCPICSWAATKRRIAEESDPSPT
                     GRRR"
     gene            31518..31919
                     /locus_tag="PLZ72_01515"
     CDS             31518..31919
                     /locus_tag="PLZ72_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35001.1"
                     /translation="MKRVTALIAALAVPAILLVGCGGDDSADSGSKSDSSSQSSDSGS
                     SDSGSSDSGSSSSTGNAKVDKFCDDAKALAEKSKKAIADRDTDLAQEAAKDAQALSQQ
                     AQDLTSEVIKDPKLATALTDCTKELANVGQG"
     gene            complement(32008..33213)
                     /locus_tag="PLZ72_01520"
     CDS             complement(32008..33213)
                     /locus_tag="PLZ72_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35002.1"
                     /translation="MPEPMTTPPQKVRARPRRTTRFAAFVAMATVAVLAVVGAVACSS
                     DDDSSPWPSVSGASTAAPTTASTTTTEAPIAPVPSPGCRNRGEAPAVTDQRREIDVDG
                     TTRWYLLTTPGPAATSSSTSTSTSTSGAAEPDPLPLLLDFHGLAEGAEVHTRMSGFDE
                     IAARERFVVAYPNGTGTPVHWAVETEDNPDLDFTDQLIDALGSELCLDMSRVYSTGLS
                     NGAIFSSLLGCVRAERIAAIAPVAGVQYPDGCQTDRPVPVLAVHGTADPILQFNGGVG
                     GFLAGEDPPEESSGGGPDGSASSSSIAGVDIDGPGYPEAVRKWAAHNGCRPKPADTEP
                     VPDVTLRTYDCPPGGEVEFYVVHGGGHSWPGSAFSETIEMVVGPTNMALPASQLIWDF
                     LSAQRLPAP"
     gene            complement(33269..34441)
                     /locus_tag="PLZ72_01525"
     CDS             complement(33269..34441)
                     /locus_tag="PLZ72_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA35003.1"
                     /translation="MDDLGDAPASAPLLSVVTPTFNESENLPILVEELHAALDGIDHE
                     IVVADDDSPDGTWRVAEEIARTDPSVRVMRRFHDKGLSAAVLDGMSIARGEYLAVIDA
                     DLQHDPSALPAMLDELRSGADVCVGSRATEGGGYGDWSASRRFVSWVATLIARVLLRV
                     PVSDPMSGFFMISESAYARAAPSINPRGFKILLEFIGRNRDLRVAEVGYTFRTRTHGE
                     TKLNGSVIRSYLLGVAELRLGRQVDPVFLLYVLVGLVGLGVNSLVFTVAEAAGVPTIT
                     TGLNDAIDPIEGSFALSVIVSILVLFVLNNEFTFWEERYRGWKLAPAFGVFFLMSLIG
                     TLVHVAVFKYLQSIGFLLSVLGADWSRVAQNLIGAVVALIVNWYLNSTYLWRRRQR"
     gene            34440..35219
                     /locus_tag="PLZ72_01530"
     CDS             34440..35219
                     /locus_tag="PLZ72_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNA35004.1"
                     /translation="MASARYRPLSQLPRTLIAVCGRFVSSSPPDEIARYFDVDETSEQ
                     ALEHRANFNTAPTTDVFVVYRDGSTRRLDTFHWGLVPRWAKDLSIGSRMINARAETVA
                     TKPAFRHAFARKRCILPVDGFYEWKAPPPGQKRKQPYFIHRPDGEPYAFAGLWEQWKG
                     PGPDGDEVTVRSTTIITGAANTAMSAIHDRMPIILAPSDWESWLSPENTDTEMLGRLL
                     VPAPDRLITMHPVSTDVNNVRNKGEHLADEVDPVLDDGRLL"
     gene            complement(35245..35685)
                     /locus_tag="PLZ72_01535"
     CDS             complement(35245..35685)
                     /locus_tag="PLZ72_01535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNA35005.1"
                     /translation="MTEQPTPSVFTMIIRGDIPGRFVWRDDDVVAFLTIGPIRPGHTL
                     VVPVEQIDHWTDVPAELWARIGEVQQAVGRALMDAFSPVRVGSIIAGLEVPHCHVHLI
                     PIDAESDLSFANVDPDPDPAALDAAAERIRGALRAAGHGAHVPG"
     gene            35726..36397
                     /locus_tag="PLZ72_01540"
     CDS             35726..36397
                     /locus_tag="PLZ72_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNA35006.1"
                     /translation="MSDTTAGVGPAAETGGGGAPGVEPSGDRLATLPNLITLVRLLCI
                     PVFLYLLFVRDARLGAAILLGVLGATDWVDGYAARHLNQVSNFGKAFDPTVDRLLLIV
                     GVVSIIVVDGCPLWFGLVVVAREVILSIWVVSIMALGAKRMDVTWWGKAGTFGIMIAF
                     PAFLAASDEGIPEAWSSFFELVAWCAAVPGLVFAMIAFVGYFPAGVRALREGRAARHR
                     AAAGV"
     gene            36513..38999
                     /locus_tag="PLZ72_01545"
     CDS             36513..38999
                     /locus_tag="PLZ72_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA35007.1"
                     /translation="MKAVIMAGGEGTRLRPLTSNSPKPMLPLVNRPMMEHIIDLLVRH
                     GIDDIVVTVAFMANNIRNYFGDGSEFGVKISYASEETPLGTAGSVRNAMDELDDTFLV
                     ISGDVLTDVDLGAVIQTHREQEALATIGLVRVENPLEYGIVITRDDGTIERFLEKPTW
                     GQVFSDTINSGIYVLEPKVFDYIAADISVDFSGDVFPALLDDGEKLVGAVLDGYWEDV
                     GTLPAYVRAHKDILDGRVQVGIPGFEMSDGVFIAEGAEVHPDAVLTGPAVVGDNCFVD
                     AGARLGEYTVLGQGVRVRSGAELERTVVHENAYLGESVKLRGTVVGRAADLRRNARCE
                     EGVVLGDEVFVGEDAVISTDVKVYPFKTVEGGAVVNTSLIWESRGARSLFGRGGVTGL
                     ANVDITSELAAKVALAYATSLKRNTTVVVSRDSSRSARMLKRAMMSGLNAGGVNVMDL
                     EVASVPLTRFHCRGVAVGGAVSLRLSPEDPDAVVIRFFDTDGVDITEEQQRKIERLFG
                     REDFRRVRPADIGDIDLVPRALEQYAVALEQTIDVKAVADRRFKVVIDYSYGSTSFAM
                     PNVLSKLSAEVLALNPFASTSGALSFERDRHAAGVAELVRASGADLGAVLDPSGETLA
                     LVDDTGTVLTDDEMLFCFLDLVCDRLLGDSVALPVTAPRAAAQIVADRGYHVRWTKTS
                     TAALMEAASEPGVGFGANREGGVILPGFLPAFDAAGGLLKMLDLLAARQVQLSEVRAA
                     APVAHLLHEAVVTPWEQKGTVMRSLVEQTHGRDVDLIDGIKVHHATGWVLVLPDPEEP
                     ITHLWVEGDTLAEARTLVQEYARRIRQMLR"
     gene            39040..39420
                     /gene="gcvH"
                     /locus_tag="PLZ72_01550"
     CDS             39040..39420
                     /gene="gcvH"
                     /locus_tag="PLZ72_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNA35008.1"
                     /translation="MNVPDDLYYSSDHEWIRVEDGRFRIGITDYAQDALGDVVFVQSP
                     DVGAEFGAGDTFGEVESTKSVSEVYAPVQGVVVEVNGDLEVSPELVNSDPYGAGWLIV
                     VEPVDLRSVETLLDADAYRKLTEA"
     gene            39417..39902
                     /locus_tag="PLZ72_01555"
     CDS             39417..39902
                     /locus_tag="PLZ72_01555"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNA35009.1"
                     /translation="MNAAGPDETTDGPLCPVCSSVNAAGANFCSACGNELPEADDSAT
                     GTYPVLGVDVPDAGEVGQLVVIRGPTAGSRYALDEDVVTIGRHPDSTIFLDDVTVSRR
                     HAEVRRVEGGRFMLADVGSLNGTYLDGDRVEEAVLREGAQVQVGKYRLVFVIGTLGGG
                     A"
     gene            39899..41134
                     /locus_tag="PLZ72_01560"
     CDS             39899..41134
                     /locus_tag="PLZ72_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNA35010.1"
                     /translation="MTADGLTSSVHTPVDAAAATAVRPAHRPIGEVLSLLQEEFPDVT
                     ISKIRFLESQGLIQPERTASGYRRFSASDVDRLEWILRQQRDHFLPLKVIRKMLDEGV
                     DRYDPGGGSQPTLFTAAIDASVGEDDEPEASDEGAGPDPDAGTGTPPRSPAHPAVASA
                     PPRPPTPSRQPPRPAERAPVPAGADPVGAVRSSAPATAAGGGGSPTPSAPTPSDPSSR
                     PAPARSATAEGAGSEPPEPARPPHETPADVVAALQEDPRPASRAEPRGARTSDRPVRT
                     RPSREPVAADTALGAGELCAQVGISEALLADLERFGLVQSVPRGGSPVYGPAALEVAR
                     LASRYAELGVEPRHLRMYLVAAEREAGMVEQLAVPLLKQRNPQAQQNAAALAGELAEL
                     GAELHRSLLRREVGSDLLP"
     gene            41184..41675
                     /locus_tag="PLZ72_01565"
     CDS             41184..41675
                     /locus_tag="PLZ72_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNA35011.1"
                     /translation="MIELELVAVRVELPSNTPVLVLRELVGRRRQLTIFIGNPEATAI
                     AFALEGVDTPRPLTHDLFCDVLDGLGTSLERVVITELRETTYYADLHLSSEDGPVTVS
                     SRPSDAVALAVRTGCPIFSEETVLDEAGFVEEVDDEDVDDDEVVEEFRHFIDNVSPDD
                     FAS"
     gene            41729..42229
                     /locus_tag="PLZ72_01570"
     CDS             41729..42229
                     /locus_tag="PLZ72_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNA35012.1"
                     /translation="MTVMPGDSASDVSYSGRSAAEIVGISYRQLDYWARTDLVRPSVT
                     DAAGSGSRRRYSYSDLLELKVIKSLLDAGIRLESVRQVFDYVREHLGEDIGSANIVIS
                     GTSTVLVRSGDELIDVLRKGQGVLNVLALSGVKDEVDARIVELFPVATAEDRTHHPSA
                     AGAGAG"
     gene            42238..>42798
                     /locus_tag="PLZ72_01575"
     CDS             42238..>42798
                     /locus_tag="PLZ72_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HNA35013.1"
                     /translation="MRTSPLDGVHRSLRARMVEFGGWDMPVSYPSGTLEEHRLCRQTA
                     VMFDVSHLGTVRVEGPGAFDSLQRELTNDLTKIGPGRAQYTHLLDPDDASVLDDIIVW
                     WLPDTDGVDRFDVMPNASNTDRVVAAFAADGVDVVDTTSGRAVLAVQGPTSRQVVATV
                     SAEAAAVGRFRVIEADVAGHRCLVAGT"
BASE COUNT         6057 a        15074 c        15510 g         6157 t
ORIGIN      
        1 accgggcgcg gggccgggtc ggtgccgtcg ggcccgcaga cccgcccgag ctcgggcccg
       61 tccccgaggt cgggccggac ggcgtgccgg tcgcggccga cgcgttcctg acccacctgg
      121 cggtggagaa gggccgctcc gagctcacgc tgcgggccta ccgccaggac ctgcgccgat
      181 acgccgggtt cctcgcccgt cgcggcctcg acgtcgacga cgtcaccgag gcggacgtgc
      241 aggcgttcgt caccgagctg cgctccggcg agctggccgc cgcgtcggtc atccgcatcg
      301 cgggcaccgt ccgggcctgg caccgcttcc gcgccgccga gggcgacagc ccgagcgacc
      361 ccacctccgc ggtcgaggtc ccacggcggc cctcggccct gccccgggcg ctgtcggagg
      421 ccgacgtcgg gtcgctgctc gacgccgtcg actcggcgtg tcgcatgccg tcgggcgacg
      481 ccgtggccga cgccctggcg ttgcgcgacc gggcgctgct cgaactgctg tactccagcg
      541 ggatccgggt gtcggaggcg tgcgggctcg gattcggaga cctcgacgtc gattctgcgt
      601 tcgcccgggt cctgggcaag cgctccaagg agcggctggt gccgatcggg agacccgcgc
      661 tggcggcgct ggccgactgg ctcgaccgtg gacgaccggt gctgctcgga tcccgggcgg
      721 tcgaccgcga cgccgccgac gcgatcttcc tcggacagcg gggacgccgc ctgggccgtc
      781 aggcggcctg ggaggcgatc aggcgctggg ccgcggccgc gggactgcgg ggcgagatca
      841 gcccgcacgt cctgcgtcac tcgtgcgcga cgcacctgct cgaccacgga gccgacatcc
      901 gcaccgtcca ggagctgctc ggtcacgcct cggtcagcac cactcagatc tacacccggg
      961 tggcgaccga gcggctggtg caggcctacc tcgacgctca cccgcgggcc cgggtcgcgg
     1021 ggtcggcgag cagggtcggc gagcggtcga acggcggacc ggtgacccgg tgaacgcacc
     1081 ggagctccag ccgacccacc ggtcccgccg gttcttcgcg tcgctgtcgg gcgcggcacc
     1141 ctcagaggag gacgccgcgt gggccgaggg gtggttgagc gaccacgagc gtgccgcgtt
     1201 ccgcaagatg ccaccggcgg accggcgcca ctcggtggcg gtggcccgtg ccgtggtcgc
     1261 ccacctcgac cggctcgggc tgaccgaagc cgacccggac gcccgctgga tcgtggtcgc
     1321 cgccctcatg cacgacgtgg gcaagtcggt gaccggtctc ggcacctacg ggcgggtggt
     1381 cgcgacgctg tcgggctggg tggggggcca tgacatggcg gcgtcgtggg ccgacacccg
     1441 tggattcacc cgcagggtcg gtctctacct gcagtacccg gagctgggcg ccgacgtgct
     1501 gcggatggcc gggtccgacg accgggtcgt ggcgtgggcg gccgagcacc accatcccga
     1561 ggaggactgg acggtcccgg tcgaggcggg tcggttgctc cagctggctg acgacggccg
     1621 tctctgaccc agccccaatt ccgttctgta gtgcgagatc caggcgaatg cctggatctc
     1681 gcactacaga acggggcggg ggagcaggcc gacgttgcgc acggcgcggt cgtagacgtc
     1741 ggccgcgacc gcccgggcct tctctgcccc gatcgcgagc agctcgttgg tcgccgccgg
     1801 gtcctcgacc agctcgcgga accgcgcctg gatcggctcg agcagcgcga cgaccgcgtc
     1861 ggcggtgtcg accttgagcg ggccgtactg ggagtactcg tcggccaggt cctccgggga
     1921 ccgtcccgtc gccgcgccca ggatcgacag gaggttcgac acgcccggct tggcgaccgg
     1981 gtcgaaggcc acggtgccgt cggagtcggt gaccgcccgc ttgaacttct tggccgtcac
     2041 cgccaggtcg tccatcaggt acacgacgcc ggcatcggac gtcgccgact tggacatctt
     2101 gtcggtcggc tcctgcaggt ccatcacccg ggctccggct cgggggatgg tggcctcggg
     2161 cagcacgaag gtctcgccgt agcgggcgtt gaagcgctcg gcgacgtcgc gggtcagctc
     2221 gatgtgctga cgctggtcct cgccgacggg gacccggtcg gtgtcgtaca ggaggatgtc
     2281 ggcggcctgc agcgccgggt aggtgtagag cccgaccgac accaggtcct gcttgccgcc
     2341 ctggcgggcc gacttgtcct tgaactgggt catgcggttg agctcgccca tggcggtcac
     2401 gcactggacg agccaggcca gctcggcgtg ctctgagacg tggctctgca cgaacagcgt
     2461 gcacacgtcg gggtcgaggc cgcaggcgag aagggtctgg gccatctcca ggctgaacgc
     2521 accgacctcg ccgggggcct tggggacggt cagggcgtgc aggtcgacga tgcagtagaa
     2581 gctgtcggcg tcgtgctggt cctggaccca gttgcgcacc gcccccagga ggtttccgag
     2641 gtgcaggtcg ccggtgggct ggatgccgga gaagacgcgg ggcatgggcg gcgatggtac
     2701 cggccggtgc cgtgggcccc gacacgggtt ccgggcgccg acccggcgaa cttgtagcgg
     2761 gcaggtcgtc cggtgaccgg tggacgacaa gttcggagcg cgccggtgaa cttgtagcgg
     2821 gcaggtcgtc cggtgaccgg tgggcgacaa gttcggtggg tggacgggga aatgacacgc
     2881 gtgtgacttg gtgggcgcga tctgctctag gctgtcgccc gtgggttacg ccgtcagcac
     2941 accggtattc gaaggtccct tcgacctgtt gctgcacctg atcctgcgcg agcaggtcga
     3001 tctctacgac atctcgctgt ccaccatcgt cgacgcctac ctggccgagc tgaaccggat
     3061 ggagcacctc gacctcgagg tggcgaccga gttcctgctg atcgccgcca cactggtcga
     3121 gctcaagacc aagcgcctgc tccccggcga cgacgacatc gacgtcgacg aggacctgtc
     3181 gctgtgggag gagcgcgacc tgctgctcgc ccgactgctc gagtgcaaga ccttcaagga
     3241 cgcggcgggc acgctggccc acctgcacgc caccgcggcc cgctcggtgc cgcgcaccgt
     3301 cggccccgac gaccgcttct gggacctcac tcccgacatg ctcgagggcg tcacacccga
     3361 gcagctcttc gcggcctacc agaaggcgat caccccgcgg cccaccccgg tgatcgacct
     3421 gttccacgtc gccccgatcc gcaccagcgt gatcgacacg gtggccgacc tctgcgtcga
     3481 gctcccccgg cggggagcgg tgtcgttccg cgagctcacc tcctcgctgg tcgaccggct
     3541 cgacgtcgtc gtgcacttcc tggccgtgct cgagctgttc aagcagggcc tcgtcgacct
     3601 gcaccagccg cagacgttcg gagacatccg gatcagctgg atcgccggtg acagcgacgc
     3661 cctgggcgac ctcgccctgg tcgatgccta cgatggctga catgccagac ggagcgggac
     3721 ccgcagacga cccggccgag accgaggccg catccccgga cggccccgat gacctgtcgc
     3781 ccatcggcga cgaggctcgc cgggcgctgg aggctgtgat catggtcgcc gaccagccga
     3841 tcgaggcgtc gatcctggcg cagctggtcg agcgctcgcc ccagcaggtc gaggacctcc
     3901 tggccggact cgttgcctcc tacgaggagg acggtcgggg tttccagctg gtccgggtcg
     3961 ccggcggctg gcgctaccag agccacgagg acctgtcacc gtacgtcgag cggttcgtcc
     4021 tgagcggcca gtcggcccgg ctgtcggccg ccgcgctcga gaccctggcc atcgtggcct
     4081 acaagcagcc catctcccgg gcgcaggtgt cggccatccg gggcgtcaac gtcgacggcg
     4141 tcatgcgcac gctccagcag cgcggctaca tcgccgaggt cggcaaggac ccgaccccgg
     4201 gcaatccgtc catgttcgcc acgacgccgc tgttcctcga gaagctgggt ctcaacgacg
     4261 tcgacgagct cccgtcgctc ggcgacttca tcccgggcgc agacgtggtc gagctgctcg
     4321 aacagggtct tcgggccgag gccgacaccg atccgactcc gcagggcgct cccggtgacg
     4381 cacccgaggg cccggacccg gccgccacgg tcgaagtggt cgatgcagcc gacgcggtcg
     4441 atgcggcggc cgaggtcatc gacgtcaccg accacgcgcc gatcgacgtc gacgacctga
     4501 tcggcgacgg cgacccgctc gccgaggggt gaatcagaac ccgacgcccg gcttcgtgcc
     4561 cgccgacgag ggcgaacggc tccagaaggt cctggcccgg gccggcttcg gcagccgtcg
     4621 ggtctgcgag gacatcatcg acgccgggcg cgtgaccgtc gacggtcgga tcgccgtcct
     4681 cggtgcccgg gtgagggtgg gggaccagga ggtggccgtc gacggcaccc cgatcggcat
     4741 cgctccggac ctggtgcact acctgctcaa caagcccgcc ggagtggtga cgaccgcatc
     4801 tgacacccac ggtcggtcca cggtcatcga catggtcccc gaccagcctc gggtgcaccc
     4861 cgtcgggcgc ctcgacctgg acaccgaggg gctgctcatc ctcaccaacg acggggtcct
     4921 cacccaccgg ctcacccatc cgagtttcgg ggtggagaag gagtacgtcg ccgaggtcga
     4981 gggccggccg gcacgagggg cgctgcgtcg actccgcgac ggcgtggagc tcgacgacgg
     5041 catgaccgca ccggcacggg tgtcggagat ccaacccggt gtgctgcgcc tggtcatcca
     5101 cgagggccgc aaccgtcagg ttcgccgcat gtgcgaggcg gtcggccacc cggtccgccg
     5161 actggtgcgg acccggatcg ggccgctgcg cgacccgcag ctgggccccg ggaactggcg
     5221 ggagctcaca cgcgacgagg tgcgggccct ggagcgggcc gccgcgccgc tcggtcccga
     5281 cggggagcga tccgccggcg caaccggttc cggggcgcca tcgggccccg ggtaacctcg
     5341 acgggtgccg acgacctccg tgctggccct ccgcggggcg acgaccctcg acgccgacga
     5401 gcgcgaccac ctcctcgagc gtgtccgcga gctgctgacc cagatgttcc ggaccaacga
     5461 cgtcgaccac gacgacctga tctcgatcct gttcaccgcc acccccgatg tccacagcgc
     5521 cttcccggcg ctggcggccc gccagatggg tctgggcgac gtcccgctga tgtgcgccca
     5581 ggagctcgac atcgacggcg ccaagccccg gtgcatccgg gtgatggcgc acatcaactc
     5641 cacgcggacc cgctccgaac tgcaccacgt ctacctgcac gacgcgcggg acctgcgcga
     5701 cgacctcccc cggtgaggcg gcggtgaccg ctcccgcccc ggacgccggt tccgctgagc
     5761 cggccgccgc ggcagccgac gacacgggag tgccgatgcg tcgtcgggcc ggcctggtcg
     5821 gcaccgggct gatcggctcc tcgatcggca aggcgctgcg tgagcggggg tggcacgtca
     5881 ccggatcgga ccgtgaaccc gctcgggcga ccgccgcgct cgacgccggg gccatcgacg
     5941 cgatcggaca cgaccctgcc gccgagatca ccttcgtcgc ggtgccggtc ggcgccgtgg
     6001 ccgattcggc ccgacccgcc ctcgccgacg ggggagtggt caccgacgtc ggcagcgtga
     6061 agtcgccggt cgccgccgcg ctcccgcact cacgcttcgt cggcggccac ccgatggccg
     6121 gctccgaggc ccagggggtg gccggatccc ggtccgacat gttcgagggt gccacgtggg
     6181 tgctgacgcc gaccgaggcg accgaccctg cggcgcaggc actggtgcac agcgtggtgc
     6241 gctcgctcgg cgccgaggtg gtcacgctct cgcccgagga ccacgaccgc ctggtggcca
     6301 ccgtctcgca cgtccctcac ctcaccgcgg tgacgctgat ggggctggcg tcccagcgcg
     6361 ccgagcacga cgccgcactg ctgcgcctgg ccgcgggcgg cttccgggac atgacccgga
     6421 tcgcggcggg cgacgccgac atctggatcg acatctgcga cgacaaccgc gccgcgatcc
     6481 tcgacgtgct cgacgacctg atcgcccgtc tcggtacgat gcgcgacgtc gtcgaccggg
     6541 gcgacggtcc gagcctcggc gagcagctgc tcgccgccca ggaggcccgt cggaacctgc
     6601 cgaccggggc gcctccggcc gaggagctct ccgaggtccg cgtggcgatc cccgaccagc
     6661 ccggcgagct cgctctggtc accacgctgg ccaccgagct cggcgtcaac gtctacgaca
     6721 tcgaggtcgt gcacgccgcg ggcgaacggc ggggcatgct cgtcctggtc gtgtcgacct
     6781 cacgcgccga ggcactggtc gacgccctgc ggggccgtgg ccgggtcgcc tccacccagg
     6841 agctgacgtg agcttcgcgg tgggggagcg acggtgaccg cggatcccgc tcggtcgtcg
     6901 tcggcgggcg cggacgaggt ccgggagatc gtgcccgtcc cggggccgct cgacgcggtg
     6961 gtgcggcccc cgggctccaa gagcgtgacc aaccgggctc tggtcgccgc ggcgctggcc
     7021 cggggcacgt cgaacctggt cggaacgctc gacgcggatg acaccgcggc gatgctcgac
     7081 tgcctccgtg gcctcggcat accgatcggc gacggctccg acggctccaa cggctcgatc
     7141 cgggtggtcg gctgcggggg ccgacccccg ctcgacggcg ccatcctcga cgcccggctg
     7201 tcgggaacga cgtcccggtt catcgccccc gtcgcggctc tggcgcgcgg caccgtcgtg
     7261 ctcgacggtt ccgatccgct gcgacgtcgc ccaatggcgg cgctcttcga cgccctcgag
     7321 gcgctgggtg cggggatcga acccctgggc gagccgggac acctgccggt gaggatcacc
     7381 gggtcgcccc gagacgtgct cgggggctcg gtccaggtga ccgggaacgt gagcagccag
     7441 ttcctgtcag cgctgctgct gtcggggccg tgcttcgacg acgggctcac cgtgggcgtc
     7501 acgacggacc tggtctcggt gccgtacgtg cagctgacgc tggacgtgat ggccgcgttc
     7561 ggggcggcgg tcgaccacga cgacgggtgg cggacgatca ccgtgtcccc gggcggctac
     7621 cgggcggtgg agcgcttcga catcgaaccc gacgcgtcgg ccgcgtcgta ctgggccgcc
     7681 gccgcggcga tcgccggagg gtcggtgcgc atcgacgggc tgggccggtc gtcgcgccag
     7741 ggtgacgtgg ccttcatcga cgtgctcgcg tcgatggggg ccgaggtcac ctggtccgac
     7801 cacgacgtcg tggtgcgcgg cgtcggcacc ctgcgagggg tcaccgtcga catggccgac
     7861 atctccgaca ccgcccagac gttggccgcg gtggcggtgt tcgctgaggg tcagaccacg
     7921 gtgacgggga tcgggttcat ccgaggcaag gagaccgacc ggatcgccgc ggtggtgagc
     7981 gagctccggc gcctgggcat cgacgcgtcg gaggaccccg acggcttcac cgtgcggccg
     8041 gggccggtgc gtccgacgga cgtccacacc tacgacgacc accgcatggc gatgagcttc
     8101 gccctcgtcg gcatgcgagc cccgggcgtc cgcatcctcg atcccggatg cgtcgccaag
     8161 acctacccgg ggttctggga cgacctggcg cgcgtgtcgg gtaccgcttc tctctaggat
     8221 cagaacccgt ggaaccgagc gacgtccccg tcatcgccat cgacggcccg gccggatcgg
     8281 gcaagtcgac cgtggcgcgc cgcctggccg accgcctgga cctgaagtac ctggacaccg
     8341 gcgcgatgta ccgcgcggtg gcgttcgccg ccatccgcca gggactcgac ccggtcgacg
     8401 tggagccggt cgccgcactg gcgactcacg tcgatctcgt ggtcggcgac gatgcggtcg
     8461 tggtcgacgg ggtcgacgcc acggtggaga tccggggacc cgaggtgtca cgggcggtca
     8521 gcgtcgtcgc cgccaacccg gcggtccgcg aggagatgcg cacccgtcaa cgggtgtggg
     8581 tggtcgagaa ccgcggcggg gtcgtcgagg gccgcgacat cgggaccgtg gtgttccccg
     8641 acgcggtgct gaaggtgtac ctgaccgccg atccggcggt gcgcgccgcc cgccgggcca
     8701 aggagatgac cgacctcgag tacgacaccg tcgccgccga catcgcccga cgcgacgctg
     8761 cggaccaggg ccgctccgac agtcctctgg tggaggccga cgacgccgtc accgtcgaca
     8821 ccacggggct ggggatcgac gaggtggtgg agcagatcgc ctcgatggtc gaatcccggc
     8881 tggccgatcg ggacgccacc gggtgagtcc gatggggacc gaggccggcg gggggaggtc
     8941 acgatgagtc gggtggtggt gcgccgcgga gcgttgcgtc cgatcagcac cggcggccgt
     9001 ctgagctacc ggatcatccg ggcgatcggt cgggtcctga tcgctccgct gtggagggtg
     9061 tcggtcgagg gtcgcgagcg gcttccggtg ggggtggcct acgtggtcgc gccggtccac
     9121 cgctcgtatg tcgacttcgc tgtgctggtc gcggcgatgc cccgcgagat gcggttcatg
     9181 accaaggaca gcgtctggaa gtggcgctgg ctgggccggt tcatcgagtt caacggctcg
     9241 ttccccgtcg accgcgccca caccgaccgc gacgcgctgc gccgttgcga ggaggcgatc
     9301 ggtctcggtg acccggtggt catgttcccc gagggtcggc gaaaggacgg tcccctggtc
     9361 gaggacctgt tcgatggccc cgcgttcgtg gcgtgccgca cccgggtccc ggtcgtgccg
     9421 gtcgccatcg gcggctcgga ccgggcgatg ccggtcgggg cccgcatgat ccggccggcc
     9481 aaggtgcggt tggtgatcgg cgagccgatc tatcccgacg tgccggccgc cggccggatc
     9541 ccgctgcggg cggtggggga gaacaccgag gccctgcgca ccgaactcca acgcctctac
     9601 gaccaggtcc gctgacctac tcctcgttct gtagtgcgag atccaggcga acgcctggat
     9661 ctcgcactac agaacgggtg tcgggtgggt gtcgggcggt acgggtgctc acgcgacgtt
     9721 gatggtgacc aggactccca ggtacatcgc cgcgatcgcg cccagcgccg ccgtcgtccc
     9781 gcccacggcc agcagcgcgg gtcgcccccg ggcgatggtg tgcaggaacg ctccggccgc
     9841 ggtgaccagc accgccagca ggtgcacctc cacccacgga tgcggcagct cgtccatcgg
     9901 gatggccatg acgttccaca ggccggtgag gaccgcgacc gccagtccga tccacgccac
     9961 ccggccgaac gcggtcgcca ccgcgacccg ggactcgggt tcgagacgtc gggtggtcgg
    10021 caggacgccg gccatgacga tctggccgcc gacccacacc gaggcggcca ggatgtgcag
    10081 gaacagtcgg aacgcggtca cgaccccagc atcgcatcgt tctgtagtgc gagatccagg
    10141 cgttcgcctg gatctcgcac tacagaacgg gacaacgggg agcgggcggt cacgtgaggt
    10201 cggcgaggac ctcgctggcg gcgcggttgc ggtcatcgga ggccaccctc acggtcgggt
    10261 cggctcccag gcccagggcg cccagggcgt cgatggcgac gagcatgtcg cgcaggttcc
    10321 gcggcggcgg gaagtactcg tcctcgtcgg tgaggtggac ctcctcgacc ccgtcccggg
    10381 gagcgagggc ggcgatgacc tgctcgacga gctcctcggg agccgacgcc cccgcagtca
    10441 ccccgaccac cccgtgcagg tcgaccgaac cgtcggcccg ccgcggcagc tcatcggcgg
    10501 tgttgatccg cagcaccgtc gcgcagcccg cgccccgggc cagacgctcc agggcgacgg
    10561 tgttcgacga gttggcggag ccgatcacca ccatcgcgtc gcagcgcggc gcgatctcca
    10621 tcaacgcgga ctgccggttg gtggtggcga agcacaggtc gcttcgaccg ggggtccaca
    10681 cgtcggggaa ccgctcggcg gtcgcggagg cgacgtcggc ccagtcgcgg tgcgacagcg
    10741 tggtctgggc cagcagcgcc accggggtgt cgaagtccgg tagggcgtcg acttcttcca
    10801 ccgactcgac gcggtggatc gcctcagggg cgacggccat ggtgccgacc gcctcctcgt
    10861 ggccctcgtg gccgatgtag acgatctggt agcccttgtt cgcccggacc ttcacctcgt
    10921 ggtggacctt ggtgaccagc gggcacaccg cgtcgaccac gaacccgccg ttgtcgcggg
    10981 cggcctggac gacctcgggc gcggatccgt gcgccgacag catgagcggc cgcccgggcg
    11041 gcacctcggc cacgtcgtcg acgaacacca ccccctgctc ctcgaagcgc tgcacgacct
    11101 gctggttgtg gacgatctcg tggtagcagt acacgggtcc gtcgaaggcc cggaccatcc
    11161 aggccagcgc cttgatcgcc atctcgaccc cggcgcagaa cccacggggt gccgccagca
    11221 ggacccgctc gacggtggcg ttgctgtcgt cgctcacacc ccgagactac gggcctcgcc
    11281 gctcggtgag gattcgtggc ggcaagtacg atggtcggcc atgtccctgg ccaccgtcct
    11341 ctccgtcgcg cccggatcgc cggccgccga gagcggtgtc gtcccgggag acgagctggt
    11401 ctcgatcggc ggccggcccc tgcgcgacgt gatcgagttc cgggtcctct ccgacgaggc
    11461 cgacgtcgag ctggagcttc gtcgaggcgg cctcgagctc aacgtcgaga tcgccaaggc
    11521 ccacggcgaa ccgctcgggt tggaggtgag ctcgccggtc ttcgacgagg tccgcacctg
    11581 cgacaaccac tgcgagttct gcttcatcta ccagctgccg cccggcatgc gtcgcagcct
    11641 gcacctcaag gacgacgact accgcctgtc gttcctgtac gggaacttca ccacgctgac
    11701 ccgcttcacc gaggccgacc tcgagcgcgt cctggtcgag ggtctgtcgc cgttgtacgt
    11761 cagcatccac gcgaccgacc ccgagcgccg cgtcgagatg ctgcgcaacc gacgcggcgc
    11821 catgagcctg cggtggctgc gggcgctgct cgaccacggc gtcgaggtcc acggccagat
    11881 cgtggtctgc ccgggcctca acgacggcga cgtgctcgac gagaccctct gcggcgtgct
    11941 cgacgagtac cccgagctcg cggacgtggc cgtcgtaccc ctcggcgtga gtcggttcaa
    12001 caaggagccg cgcatgcggc cccacaccgt ggcggaggcc gaacgggtct gcgacgtggt
    12061 cgaccgctgg caggaggtgt tcctgtcgac cgtcgggcac cgcatggtgt tcgccgcgga
    12121 cgagtactac ctgctggccg gtcggccgtt ccccgacggc gccacctacg gcgacttctc
    12181 catgcacgag gacggcatcg gcatggcccg ggccttcgag gccgagctcg acgggacctc
    12241 cgaggtcggc atcggcatcc agtccgggtt cttccactcc gtcgacggcg cccccgcgga
    12301 cggctaccgc gcccctcgca ccgagccgac accggccccg gcgccggtcg ccctcggccc
    12361 tcgccgacga tcaccagagc cagagccaga gccagcggtc ggcccagcgg tcgagcccga
    12421 ggggcgtgtc gcggtgctga ccggtcccta cggcgcccgc atcctcacgc cgctcatcga
    12481 ggacctgctc cggcgagagg gccggggcgg tcccgacgac gccggcgtgc gggtcgtcga
    12541 ggtggagaac cggttcttcg gcggcaacgt cggcgtggcc ggcctgatgg tgggcgagga
    12601 cctggcccgg gtgctcgcgg ctcagcccgt cggtgaccgc tacctgctcc ccgacgtgtg
    12661 cctgtcccgg ggcgtgttcc tcgacggcac ccgtcccgag gacctccccc ggcccgtcga
    12721 ggtggtgccg accgacggcg tcgccctccg gcgggtcctg ctcgaaggtg tgaaccgtgg
    12781 ctgagttgcc gatcgtcgcc atcgtcggcc gacccaacgt cggcaagtcg accctgatga
    12841 accggatcct cggccagcgg gtcgccatcg tcgaggagaa gcccggcgtc acccgggacc
    12901 gcaaggaggt cgaggccgag tggcagggcc gaccgttcct gctggtcgac accggcgggt
    12961 ggatggtcgg cggaagcgac ctcgaccgca aggtctccaa ccagtccgag caggcgatca
    13021 aggacgcgga cgtggtgctg ctggtggtcg acgccaaggt cggcgtcacc ggtgaggacg
    13081 cgcaggtcgc ccagctgctg cgggccctgg atgtgccggt gcacgtgctg gccaacaagg
    13141 tcgacgacac cagccacgag gcgctgatct gggagctcat gtcactcggc ctgggtgagc
    13201 cgctgccgat ctcggcgctg cacggtcggg gcaccggcga cctgctcgac gtgatcatcg
    13261 ccgagctgcc cgaggtcccc gacgacgacg tcgagcagtc cgacatggac gagggcgagg
    13321 gcgtcatctc cgtcgcgctg gtcggtcgcc ccaacgtcgg caagtccacg ctgttcaacc
    13381 gtctgatcgg cgaggaccgg gcggtggtgc acgacatgcc cggcaccacc cgcgacgccg
    13441 tcgacaccgt ggtcgacacc gagatcgggc cgttgcgctt cgtggacacc gccgggatgc
    13501 ggcgcaagtc ccgcatcgac gagggcaccg agtacttctc gatggtccgg gcgctcaagg
    13561 cggtcgacga gtccgacgtg gcgctcctgg tcatcgacgc caccgagggc gtcacccacc
    13621 aggaccagcg cctggccgaa cgggtcgacg gggcgggatg tccgatcgtc gtgctgctca
    13681 acaagtggga gctgctcgac gaggaccgcc gcaaggacgt gctctaccag ctgggtcagc
    13741 gcctgcactt cctgggcgac tccgccgtgc tgcgcatctc ggcgctcacc ggccggggcg
    13801 tgcaccgact gctgccggcc ctcgaggact cgatcgacgc ctaccacacc cggatcccga
    13861 cccgtcaggt caacgacgtg atccgccggg cccagcaggc ccagcccggc ccgcacggcg
    13921 cccgggtcct gtacgccacg cagggagcgg ccgatccgcc gaccttcacc ctgttcgcca
    13981 acaagacgct gccggccagc tacctgcgct acctggagcg ccagctccgc gaggcgttcg
    14041 gcctggaggc cacgccgatc aagatgcggg tgcgtcgccg gggcggctga ggccgcgtgc
    14101 gctcccgccg cgaggtcgcg ccgcaggcgt gctgagatcc cgctgagacc ggatcgctgc
    14161 ggttgtcggg acgacgggag ccacccccta ccatcactgc gggccgaggt ggcccgccgg
    14221 tacccgagga ggaccatgag ccaggttcgt gacgagatcg aggcgcgggg caagccccag
    14281 ctgggcgccg tggacgcggt gaccgagctg gtggtcaccg acgaggtcga gggccagggc
    14341 gacgaggtca ccgccggggc caccgtcacc gcccactacg tcggcgtgtc ggcctcgacc
    14401 ggcgcccagt tcgacgcctc gtgggaccgg ggcgcaccca tcagcttccc gctcgacggc
    14461 gtcatccgcg gttggagcga gggcctgctc ggcatgaagg tcggcggccg tcggaacctc
    14521 gtcatccccg gcgacatggc ctacggcgcc aacccgcccc ccggtgcggg catcgccccc
    14581 aacgagacgc tcgtgttcac cgtcgaccta gtggacgtgt cgtggcgctg agcccgggcc
    14641 ggtcgcggcg tcgtcgggga gtggcgttgc tggccgccgg agcggtcgcc ctcggcggac
    14701 tcgccgtggc ctgcggcgac tccgagtccg ccaccacgtc gaccacgatc gagtcgaagg
    14761 gcgcggccgg gacctccggt tccgggtcgt cgtcgggagc cgggtcctcg gacacgagcg
    14821 caccggcggg tgcgaacagc gagttcgccg ccgagatcga ggcccggggc gcgccgacgc
    14881 tcgccgcggt cagcggtccg gtcaccgagc tgaaggtcac cgacgacgtg gccggcaccg
    14941 gcacggccgc cgaggccgga tccaccgtca ccgtccagta cgtcggcgcg gtggccgaca
    15001 ccggcaaggt gttcgacgag tcgtggtcga gcggtcaacc ggtcaccttc ccgctcgacc
    15061 gggtcatccc gggctggagc cagggcctgg tcgggatgaa ggagggcggt cgccggaccc
    15121 tcgtgatccc cgccgagctg gcctacggcg ccaacccgcc gcccggctcc ggcatccccg
    15181 ccgacgccgc gctggtgttc accgtcgacc tggtgaaggt ggactgaggt ggcggagccg
    15241 ggcgacgagt cgcttgccgg cgacggttcc gagcgtccct cggggccggt cgcccggttc
    15301 gcgtccgcgt cggcccgggc ccgggccggc aacctggagc ggggcggcga gaagctggcc
    15361 gcccagggca agctgttcgt gcgggaccgc atcgacctgc tctgcgaccc ggggaccttc
    15421 gtcgaggacc agctgctcgc caactcgccg taccgcgacg acatgagccc ggatctgccc
    15481 gccgacggcg tggtcaccgg ggtgggagag atcgacggcc gcccggtcgc ggtgatggcc
    15541 aacgacccga cggtcaaggc cggcagctgg ggcgcccgca ccgtcgagaa gatcgtccgg
    15601 ctcaccgaga ccgccctgcg cgacgagatc ccggtggtgt ggctggtcga ctccgccggg
    15661 gcgcgcatca ccgaccaggt cgagctgttc cccggccggc gcggcgcggg gcggatcttc
    15721 tacaaccagg tccgcctgtc ggggaaggtg ccccaggtgt gctgcctgtt cggtccctcc
    15781 gcggcgggcg gcgcctacat cccgagcttc tgcgacctgg tcgtcatggt cgagggcaac
    15841 gcctcgatgt acctcggctc gccgcggatg gccgagatgg tcgtcggcga ggtcgtgacg
    15901 ctcgaggaga tgggcggcgc cagaatgcac gccacggtgt cgggctgcgg cgacaacctg
    15961 gccgtcgatg acgaggacgc catcgagcag gcccgggcct acctgacgta cctgcccagc
    16021 cgttggagcg aggacccgcc cgcctaccac cccgtcgaac cgtcgcggcc gttgaccgcc
    16081 gagatcgtcc ccgaggaaga ggcgcagggc tacgacgtgc acgacgtgct cgacggcctg
    16141 ctcgacgccg agtcgttctt cgagatcaag ccgctgttcg cccccgagct cgtcgtgggc
    16201 ttcgggctgc tcgagggccg tccggtcggc atcgtcgcca acaacccgat gcacaagggc
    16261 ggggtgctgt tcaccgactc ggccgacaag gccgcccgct tcatctggct ggccgacgcc
    16321 ttcggggtgc cgatgatctt cctggccgac gtccccggct tcatgatcgg ctcgcaggtc
    16381 gagcgcgagg ggatcatccg ccacggcgcc aagatgatca ccgcggtcag cgaagcgacg
    16441 gtgccgaagg tgtcggtgat cgttcgcaag gcctacgggg ccggcctcta cgccatgtcc
    16501 ggtcccgcgt tcgagccggt ggcgacgatc gccctgccga cggccaagat cgccgtgatg
    16561 ggccccgaag ccgcggtcaa cgccgtgttc gccaacaaga tcgcggcgat cgaggacccc
    16621 gacgagcgcg aggcgttcgt ggccgagcag cgcgagatct acgagaccga cgtcgacctg
    16681 ctgcggttgg cctcggagct ggtgatcgac gcggtcgtcg agtgggagga cctgcgctcg
    16741 gacgtctcgc gtcggctgcg gcgggccgcg ggcaaggacc ggacgttctc ggtgcgccgc
    16801 cacggcgttc ccccggtgtg atgggtcttc gggtcctccc gggaggttga gcaggtgccc
    16861 tggtgcgagg agtgcagccg gttctacacg ccgtcgacgt tgacgcccga aggcgactgc
    16921 ccgtcaggcc accacgtcgc ggaccccgac acggccatcc accagtccgc tgcctcacct
    16981 cgcgacgacg agcacagcgc caaggtgccg tggcacttct ggctcctgtt ggccgcggtg
    17041 gtcatctacc tggggtggcg cctgatccag ggcgtccagt ggctgctggg ctgagcgagc
    17101 gcgggtctgg cccctcggtc cccggattct catgaccgtc cgcgccactg gtgaccccag
    17161 gagtcatgag gatctcgggt tggccccgtc ctccgggtgg agcgaccggg tcggtggcgc
    17221 gggttcggcg ctgcgcccga gcggcgctac agtgaccgct ccccgggctg tggcgcagtt
    17281 tggttagcgc gcttgactgg gggtcaagag gtcgggagtt cgaatctccc cagcccgacg
    17341 gtacgaagcc cctgcaaatg caggggcttc gccgcttttc gtcctgactg ctttcgagtg
    17401 ccgtcgcctg atctggggcc gtgtacgcag cacgtatgca ggaccgtcac accctctgcg
    17461 tgaggcagct gtccacagcg gacaggtgga ggtccgacga taggctggtc tggtgggcac
    17521 gctccgagcg gttgtcgaag ccaagcggag agagatcagc gatctcgtgc gccatcatcg
    17581 tggtcggtcc gtagccttgt tcggctcggt cgccaggggc gacgacacgc ccgagagcga
    17641 catcgatttc ctcgtcgagt tcgaaccggg cagctcgctg ttcgatctga tggacctgca
    17701 ggaagcgctg gggcagctcc tgggagtccc ggtcgacgtc gtgtcggtcg gcggactcaa
    17761 ggatcgagac gaccacatcc gtcgggaagc tgttccgatt tgagccgagc cgacgacctt
    17821 cggatcgccg acgtccgcga ggcggcccag cagctcgcca ccttggtcgc cgacgggcgt
    17881 gaagcattcg acaccgattg gatgcgccag cgcgccaccg aacgtttgct cgagatcatc
    17941 ggcgaggcgt cgaacgccgt ctctgacgag ttcaagtccg accacccgga cgtgccgtgg
    18001 cgtcacgttg tgaatctccg ccacttgttg gctcaccact accaccgcgt cgacagcgat
    18061 cagatctgga cgatcgcaac ggtcagcgtg ccggcgctcg ctcgggagct tcagagcacg
    18121 gacgagtagt caggacatgg cgaatcggcc ggcatcgatg tatgcaggat gtatgcagac
    18181 ctccggcgat cagccggatc cgtcatccca ggctggaacc agaaccattg gaaacaaggg
    18241 gatcgcggga caggcgacca ccagctggac cagccgacgc ggccgtagct cgactggggg
    18301 gtcaaaggtc gggagttcga atctccccag cccggccgaa ctgagcccct gcaccagcag
    18361 gggctgcgcc cctctttcgt cagcggtcga gccggtaggc gcggagcatc caggtgccgc
    18421 cgccgggtgc gggaccgaag acgagtcccc ggtcgcccga tcgcgtggcg tagggcggtg
    18481 actccgagcc cttcaacccc gtcgcggcga accagtcgcc cgaggtcggg tcgatgaaaa
    18541 ccgtgcgacg ggagccgacg gtcgcggtcg cccattgccc tcggctcagc tgaccccaga
    18601 tcgtcgacct cgctccgctg ggtccgtcct tctgctccgg agcaccgacg ccggtcgatc
    18661 cccagagcga cacgccgtca gctgctgctg atcccgatga cacgctggcg atgtacccgt
    18721 ccggagcgca ggtgatggcg tcgacccgcc cgggaacccg ctggtcggtg gcagcgccct
    18781 tgagcggtcg gacccggaac gatccaccgg aggacgttcc cgggtcggcc acggtcgtcg
    18841 gagccgacgc cgcgatcagg tgctccacgt acgcctcgtc ggtcgcgcac gtcggtccga
    18901 tcacctcgga cgtcagcgtc caagtgccgt cggggtcgcg gacgaagaat ccggtcgacg
    18961 tcgtgaccgc ggcggtgtca ccgagcgagg cgaagtcccg gggaacgccg tccacgtgga
    19021 cctccgcgga gctcggctcg agcgcgtcgc cggtgaacga gatgagtgcg gtgtgcgctg
    19081 acgggcagtc gcggtcgacg gagtcgacgg tggacaccgg ggtggcgcac gtctgggcga
    19141 gcaccaccag agcgtctccg tcgacccgtg gttgcatcca caccacgggg gcacccagat
    19201 cctccgacgc ccgggctgtc agtcggtcgc cgtccggttc gaggacgcgg acatcggacc
    19261 cgaccgcttc gatcaccgcc ggacgtgccc ccgccagcag cactgcggtg gcctgaggag
    19321 tcggagcgtt ggcgaccgac ggacccgacc acgaaccgac gacaccggtt gcagtttgcg
    19381 atggctcggc agtcgttccg ccgctgccgc acgcggtgat cccggcggcg acgagcgcag
    19441 cgaggaaggc cgcccacaag gacccacggc atgtcccccg cgctggaccg gcagcgcatc
    19501 tcacccgtcc gaccataggt ggtcgggggg tcggcaaggc tggccctcag ccgaggcggc
    19561 ggcgcaggca cagcaacggg aagaagtcgt tgtgcgagga cgggacgtcg ggtcgggttc
    19621 ccagccctcg cgctggtaga agcgacgggc ccgcgcctgg gcctcgacga cgcggagtcg
    19681 catggcggac gctccggtgg tgccggcgta cccggtggcc caaccgagca gggccgccgc
    19741 cacccctgtt ccccagaact ccggcgtcac gtacagcagt tcgagccaga cgcccctgac
    19801 attctcggtc gggtccgggc cgaccttcgc cacacccgcc ggctgcgcat ccacgtaccc
    19861 gatggcgaag cgggcgtggg gatcctcgat cgcgacaggc cagtcgtagg ccgctcgctc
    19921 ggcggccacg ggagcgagtt ggtcgggatc gacgaggccc tcgtagccgg cgagccacga
    19981 ccgcaggtag aggcccccga ccagttcggc gtcggcgacc gaggctcgcc gcacctcgat
    20041 ctgtgccggt cggtcctccg tcggttgcgg catcgtcgtg atcctgtccg cgaacccggt
    20101 gcgacgccga ggcgattgcc aacgcaccga cctcggcgcc gggtgggagg attgtccgat
    20161 ggaggatcgt cgtcccggct ccgggcgcta cgcccgcctc gagcgggagc agcggtgggt
    20221 gctggacgcg gtgcccaccg aggcccggcc tcgtgccgag atcgtcgacc gctacctgct
    20281 cggcacccgg ctgcggctcc ggcggatgac ctcgtcggat gggctcgtcc tcaagttggg
    20341 gcagaaggtc agggtcgagg caactgatcc cgagcgggtg atggtgacga gcctctacct
    20401 gagctccgag gagttcgacg tgctggcgag tttgccggcg gcggagatcg ccaagtcccg
    20461 gttcgtcctc ccgtggggca cgggagctct cgcggtcgac cgcttccacg gccggctgcg
    20521 cggactcgtc ctcgccgaga tcgaacacgg tcccgacgac gagctgctca acccgcctcc
    20581 gttcgcccgg gccgacgtca cccacgacga ccgctactcg ggaggcgagc tcgcctcagc
    20641 caccgcgtcg gagatcgccg ccctgctccg acggtagatc tggggcgcgg aatcgacagg
    20701 atctgtcgat tccgagccat gtttccggtg ggtagggttc gccatggcct tcgacgtgtt
    20761 caaggacgag accttcaact tcaaggtcca ggcgatgctc ggcgagctgc gcaccggttg
    20821 cggcgacgcg ggggagatca tgtcgaccgc gtcgtcgatc accgacggcg acgccgacag
    20881 ctgggtggct gcgtggaggg cgctcgccga gcgggtcgag aagatcgcgt cggacgccga
    20941 cgcaggcggc caccgggtca gcgcccggga ggcatacctg agagcatccg cgtaccactc
    21001 ggcggcactc gaggtggtcg acggcaccag cgatcccaac gcggtgctca agcccacgtt
    21061 cgccgcccac cgacggtgct tcgaccgcta cgtcgagctt tctgagcccc cgatcgagaa
    21121 gctggacatc gcgtacgagg acaccacgat gcccggttgg ttcgtccccg ccgctggttc
    21181 ggggcggcgg ccgacgatcg tgctcagcaa cggcagcgat gccccggtga tctcgaccct
    21241 cgggatcgcg ctggcggccc gggcccgtgg ctaccacagc atcatgttcg acggtcccgg
    21301 tcagcagtcc atgctcttcg agcgcaacat cccgttccgt cccgactggg aggcggtcat
    21361 caccccgatc gtcgacctcc tcgtcgcccg cggcgacgtg gacgacaaga agctctgcat
    21421 ctacgggttg agccagtccg gctactgggt cacccgggcg atcgcctacg aaccacggtt
    21481 cgccgctgcg gtcgtggatc ccggagtcga cgacgtgtcc gcctcgtgga tgggccacct
    21541 gcccgaggag atgaaggaga tgctcgacaa gggcgatcgc agcaccttcg accagtggat
    21601 gcagatcggc gaacaggatc agaccccggc cgagaagcag gagatcgagt ggcgtgccaa
    21661 gccgtacggg atcgaggacg ccttcgacct gttcaccgcg gttcggacct atcggctcac
    21721 accagaggac ctggcccgga tcaccatccc gctcctgatc accgagcccg agggggagca
    21781 gttctggccg ggccagtccg aacggatgca ccagcacgtg gcgtcctcgg agctggtgca
    21841 cttcaccgcg gctgaaggag cggaccggca ctgcgagccg atggcccgtt cgctgctcga
    21901 gcagcggatg ttcgactggc tggaccgaac actcaaccct tcggcctgag cgttctgggg
    21961 cgcgggatcg acaggatctg tcgaatccga cgccccagaa gcagacggtg tccggatcgg
    22021 gttgctcagc ggccgagggc gtcggcggtg acctccatgt gccccaggtg ctggaacagc
    22081 tcctccagca cgtggagcac cagcgccccg tcggagcggt cctcgccggg catgaaggcc
    22141 cggaagtcgt gctcgggcgt cgtcggcccg acggcgaact ccccgatgag cgtggaggtc
    22201 cgggcgaccg cggcgtcgat gcgggatcgg agctcggtca cggtcccggt cgctgcgaac
    22261 tccgcctcgc ggtcccggac gctgtcgcgg cccaggccgg gcatctcgaa ccaggagggg
    22321 gccagctcgc agcagtgcac gatcagcccg gccaccgagt tggtgcccca cccgtcgggt
    22381 gtccggttga cggtgtcgtc gtcgaggcgg tcgagcacgc ggttcatgcc ggcgaaggcg
    22441 tgccgtacgt acaggtcgat ggtggacggg tccagatcca tggagatccc tcggggtcgt
    22501 ggtcgggtgg gcggcttctg ggggtcggga tcgacaggag gtgtcgattc cgaccccagt
    22561 ttgcagggcg ggtgagggcg atcgctctac cgtcactcct gttccggcgc gggggatctc
    22621 gtcggacggg cgacaacagg aggggacatg cgtaggcgtc tgtggggtgt ggcgacgatc
    22681 tcgatggttg cgctggcagt ggcctgcgct cctccgacgg tgccgccgcc ggtggtgacc
    22741 aacgagtggc cggggaccgt gacccagggc cggaccatcg gcaccaccgg caccgtgtgg
    22801 ggtggcctcg accacaacga cgactggctc gttcggtccc gggccggggt cgacggcacg
    22861 ctgaccgcgg ccgagacggt gctcacaccc cggaccggtc cgggtgtcgg cgacctcgga
    22921 gccccggtgg tgctgtcgcc cacgtactac tcgcacccgc tctaccagag cccgctggtg
    22981 tcccgcccct acctggggac gcacctgctg ctagccggcg gtgacggcgt caccgcggcc
    23041 aaccggatct tcgtcgagca gggcgggacc tggaccgccg ggggcaccta cgcgctgaac
    23101 ccgaaccgcg aggtggtcgt ggcgttctcg gactcgtacc tggtcacccg tggcagcgcg
    23161 gcgtcggctg atctcctcca ggtgcacccc atcgacgtga ccggcggggt ggtcacggtc
    23221 ggtccggcgg tgtcggtgtc gctgccggcg tcgtggagcg actacctgcg gatcgacggc
    23281 gagctcgccg gggacaccct cgtcgtcgcc ggcagcgatc cgatcaggcc gggcgctgag
    23341 acggcggtcg cggtcgcgga cctgtccgat ccgaccgtga ccgccctgac gcaggtgtgg
    23401 accggcggcg acctggtgct cacctcgttc gacctcgatg tcgatggatc cgtcacccgt
    23461 gccgcggtcg gggtggaggc atcgcaggga tcgggcacgg tgggacgggt gatgatcctg
    23521 tcgccgaccg gctccgggcc gtgggccacc accgccgtcc tcaccccgcc ggccggtctg
    23581 cccgacatcg gccacggcac ctacttcggc cgtcaggtgg cgctcgacga cggcctgctg
    23641 gtcaccctgg cccgattcgt ggcgcggccc agcccggcgt cgaccggcac atcgaacgtc
    23701 ggtgtcctcg gggcctaccg cctcgtgggc ggggtgtggc agttcgaggc gtcgatgacc
    23761 ccggccccgg acgtcatcac cacgccggag accagccgtc tgcccgccgc gctcgacgtg
    23821 gcgggaacgg tggtccacgt cgagatcagc gggtccgacg agggtgcgcc cccggctccg
    23881 accttcgccg agagctggat gttcggttcc tgacccggag ccccagttct cagggtgacg
    23941 ggagggggac cgggtcccgg ggcggctccc cgcgcagaac ccgcaggagg tcctcggtgg
    24001 cccggacccg cagctcggtc accgacgcgg tggacgagaa cgccacatgc ggcgtgatcg
    24061 tcacgtccac ggcgtcgggg cctgtcatcg cggctcgcag cacctcgggc acctcgggtt
    24121 cggccggcag gacgtcgagc gcggccgcgc cgggtgtccc ggtgcgcagc gccgacacca
    24181 gggcgtcggt gtcgaccagg gcgccgcggg acgtgttgac cagcaacgac ccgggccgca
    24241 tggccgccag ctcggtcgcc ccgatcagcg gcccggctcc ggcaacggcc gggacgtgga
    24301 ggctgacgac gtcgctccgg gccagcaggt ccgccggtga caccacctcg accccggccg
    24361 gcgggtcgac caccgagcgg ttgtggccga ccacggtgca gcccaacgcc gcgaacttgg
    24421 ccgcggtggc cgagccgatc gcccccaggc cccacacgcc gacggtcagg tcgcgcaccc
    24481 gccgcaggtc ccgcatccct ggccgccagc ggccgtcgcg gacgtcgcgg tcgaacgccg
    24541 tgactccccg cgcccagttg tgcacgagcg cgacgacgtg gtccgacacc tcctcgacgc
    24601 agtagtcggg tacccgggtg acggtcaggt tccggcgggc cgcctcggga aggtcgatgt
    24661 tgtccaggcc gactcccagg cgggacgcga cggccaaccg gggtgcagcg gcgagcaccc
    24721 gggccgtcac cggcgcccag cagaacagga tccccaccgg gtcggcctcg gtgaccgccc
    24781 gcacgagggc gtcctcgtcg gccggttcct gaacgtcgac gaggtcgtag ccggcgtcgt
    24841 cgcagagctg ccgttcgatg tcggcgtcgt cccagccccg gtcggtgagc aggatcgtcg
    24901 ggcgggtcac cgggaacctc cctgcgcggt cggaccgctc ggcggccacc ggcggtgcgg
    24961 gtcgatcacc gtcgtctccg gcgccgcgac cacctcgttg tcgatgagcc ccaggccgtc
    25021 gacggcgacg ctcacccggt cgccggggcg cagccaccgg ggatcggcgg actgctgggc
    25081 ggcgccggcc ggggtgccgg tggagatgag gtcgccgggt tcgagggtga acaccgagct
    25141 gagcagcgcg atctgcgccc agcagtcggt gatcatgtcg gcggtccggc cgtcctggac
    25201 ctgctcgccg ttgacccagg tgcggatccg caggtcatgg gggtcctcga cgtcgtccgc
    25261 ggtggtgacc cacggaccgg tcggggcggt ggcgtcgaag gacttgccga gggtgacggt
    25321 gggggagcgg cgttgccagt cccgggccga gacgtcgttg ccgaccaggt agccgcccac
    25381 cacctcggcc gcccgatcgg cggggacgtg tcggcagcgc cgtccgatca ccatcaccag
    25441 ttcgccctcg tagtcgagct gggtcgagct cggcggggcc tggatcggat cggtggggcc
    25501 ggtcacgcat gtcgtctgct tgttgaagaa cagcgggaac gccgggggag cggcgccggc
    25561 gtcggcttcg tggccgcgga agttgaaccc gatggccagg aacttcggcg gccgcggcac
    25621 cggggccagg agccgagcct ccgcgagcgg ccggcgtgcc gaggccgggg cggcggcgag
    25681 cgccgcggtc actgcgtcgc ggccggcgtc gccggcggcg agcacggcga cgacgtcgtc
    25741 tccgaaggtc gcctcgacgg cggtgatgtc gacgacggtg agcccgtcga gggcagggtc
    25801 gtcggagagt gtgccctcga cgaccgccag gtgggcgccg tcgtgggtgg cgatccgggc
    25861 cagcctcatc gccgcaaccc ccgggcccgg tcgccgagcg actcctcgat gcgcagcatc
    25921 tcgttccact tggcggtgcg ttcgccgcgg gtgatcgacc cgaccttgac gccgttcgac
    25981 ccccagccga cggcgaggtg tgcgaccgac acgtcctcgg tctcgccgga tcgggccgac
    26041 acgatcgtct gcaggccccg ggcgtgcgcg gcgtcgagcg cggcgcgggc ccgggtgagc
    26101 gtcccggcct ggttgggctt gatcagcacc gtgttggcgc tgcggtcctc ggcggcccgt
    26161 gcgacccggt cggcgtcggt gaccaggaag tcatcgccga ccacctggat gcgctcaccc
    26221 gtcgccgcgg tgaacgccgc catcccggcc gggtcgtcct cggcgacggg gtcctcgacc
    26281 gacacgatcg ggtagcggtc gacccacgag acgagcaggt cgatcatcgc agcggtgtcg
    26341 agctcccgtt cctcccggcc caggcggtag cggccgttcc ggccgaactg gctggcggcg
    26401 atgtcgaggg cgatgccgac ctgggtgacc ggctcgagcc cggcggcttc gatcgccgcg
    26461 gtcagggcgt cgagcgcctg ctggttggtg tcgagcgccg gccaccagcc gccttcgtcg
    26521 gccactccca tccgcaggcc gcggtcgtcc atccagcgtc cggcggcgcg gtagatctcg
    26581 gcggtccagt cgagcgcctg ggcgaaggag gtcgcacccc gagggacgac catcaggtcc
    26641 tggatgtcga tgcgacggtg ggcatgcgcg ccgccgccga agatctggat ctcgggcacc
    26701 ggcagcgcca ccgtgccggc gacctcgacc cccggaccca gcaccgtccg ccagagcggt
    26761 tcgcccctgc tcgcggcggc ggcgtgggcg ttggcgagcg agacagcgac cacggcgttg
    26821 ccgcccaact ccgagaaccc gacggaggga tcgaggctct cgagggcggc gtcgacggtg
    26881 gtctggtccg ccgcgtcgag gccgatcagg gccggagcga tccgctccga aatgttgctc
    26941 accgcggtgg tcacgtcgag cccgtcgaag cggtcgccgc cgtcgcgcag gtcgagcgcc
    27001 tcgcccgagc cggtggaggc gcccgccggg gcgagagccc gtccggtacc gctcgtggtg
    27061 gtgacgaccg cctcgacggt gggtcgaccg cgggagtccc agcggcggtg gccggtgacg
    27121 gcggtgatgg tcgcgtcgtt catggagtcc ttcggtgtgg atggcgggga cgtgtggatc
    27181 cgcccacccg ttctgtagtg cgagatccag gcgaacgtgt ggatctcgca ctacagaacg
    27241 gtcaggggga gtggaaccag cgggtcagca ggtcggccac cgtggcgggg gtgtcgacca
    27301 acagggtgcg ggcccggtgg cgtacctcgt cgcgctgcgc gtcgtcgagg tactcgaccg
    27361 gcgatgcccc gtccacgcgg gccaacgcca gtgccgggac cagcgtggcc acccgctcgt
    27421 ccaacttgtc ggggtcctcc caggcgacgt ggctgcggta cccggcgacc agtgcatcgg
    27481 cggcccgagc cagcacggct gcatcgtcgg ggtgccacag gcccttcagc aggaggtggt
    27541 tgaggcagaa cgcgacgtcg aaggccgggt cgccccaggt cgcgcactcg gcgtcgacca
    27601 gcaccggacc gtcgggtccc accagcacgt tcttggggct gacgtcgccg tggatcaccg
    27661 tgcgaccggt ggcggaggtc cgctcggcca gatcgtcgag ccggccggcc atgtcggggt
    27721 gcgcccccgc ggtcacccgg aggtaggggt ccaggcgcag tgctccgaac aggtcgccgg
    27781 ccgcgaacgc agcggcgtca cggaccgggt cggacatggc ggcgtgcacg gcaccgagcc
    27841 ggtcgcccaa cgcggccgcc accccggcgc cgacgtcgtc ggtgagcgga ccggcggcca
    27901 gctgcgcctt ccagttgccg aaccgggccg ggtccagcca ctcgaggacg aggacacccg
    27961 acgcggcgtc gtagccgagc gctcggggca ccagggcggg aacggcccga gccgcggcgc
    28021 gcagccacgc cgcctcgtgc gcggtgcgcg aggtcgggac gatccaggtg gcctcgaccc
    28081 gcagacgctc gagggctcgc ttggccacca cggggccggt ggcgatgtcg acccgccaga
    28141 tgtccgacga gaccccgccg gtcagcggga caccgctcac gccgtcgccc tcggccagca
    28201 gtccgagggc gttcagaccg cgacggatcg cggccggcag ctcggtcccg gcggtcaccc
    28261 gagagcgccg gcgacccagt ccgcgatgag cgccgcggtc cggggacggg cgtcgtccgg
    28321 ctcgatcagg tagtggtcgc cggccacctc gacgagctgc ttgtcggcgg tgccgatggc
    28381 gtcgaggatc gtccgggcgt cgctcgggaa gacgccggtg tcggcgtccg cgttgatcac
    28441 cagcgaaggg accgtgatcc gggccaggtg gggctcggcc cggcactgcg aggtctccag
    28501 gctccacatc gacaaccatg tgcgcagcga gctgcactgc ccgacgcccc aggtcccgta
    28561 gttggcccgg cggggatcgc ccaggtagca ccggttcgct cgccgggcgg tgggctcgag
    28621 ggtggggtcg acggtgcgcg ggtcggccca caggcgggag gtcgtgaaca gccggtcgcc
    28681 gatccccgcg gcggccagtc gatcgagctc gtcccgacac cagtcggtga tccgctggtt
    28741 gcgggcctcc tgcgcggccc gatagcgagc gacgaactcg gccgagaacg gcggaccgtt
    28801 ctgggggttc cacaggtcga gggtcgggtc ggtcgcggtc gggtcggtct cgtcggtcac
    28861 cgccgcgtcc atccacgcgg tgagcacgtc gggtcggccg ggatgggcgg cgagcgacac
    28921 gtaggcgtcg gcggcgatga ggtcgtcgat gcccgcggca ggacgcatcc ccggcagcgg
    28981 ggtcacgttg gggtcgaccg cctgggactg gtaggcggcc atgagcgaac cgccgccgga
    29041 gttgccgagc agcaccaccc gggagacccc ggcctgatcg cgcagccacc gcacgccgac
    29101 gcccacgtcc gcgagggcgt gatcgagcag gaagtgggcc tcgtcgccgc ggaagcgggt
    29161 gttccaaccg aggaacccga agccgtgccc ggcgaggagg tcggccaggt agtgctcgga
    29221 gaagtcgatg ttgtagtgcg acgcgacgaa cgccacctcg ggccggaccc ccgcgggggt
    29281 gtggtagacg ccctggcacg ggtggccgcc ggcgccgaag cgtccgaagc ccggcgacgg
    29341 caatccgacg aaggtgcgct cgacgctcac cgggagtggt cgacgagtcg ggactcggcc
    29401 tgcggcgtgt ccgcctcggg caccgcggtg agcagctcgc gggcccgggc ggcgtcggtg
    29461 ggcatgccgg gcgtgtcgcg gcacagctcg atcaggatcc cgttggggtc caggtagtag
    29521 tgcgactcgc accagtcgtg gtcgaggacc atcaggacgt cgctgtcgtc ggcctcgacc
    29581 cggtcgcgca cctcggcctg cttggtctcg tccgcccgca gcgccaggtg gttgcaccac
    29641 agcggcagac cgagcccggt ggcgatctcg gtggaccatc cgggcttctc gcccgcgtcg
    29701 tggaggtcga agaacgcgat gcagctgccg tcgtcgaggg cgaagaagaa gtggcggatg
    29761 aagcccttct cgacgggggc gacctcggtg tgcaccagcg ggaaccccat gaggtcctcg
    29821 tagaagtgcc gggtcgcctc ggggtccttg caggcgaacg ccacgtggtg cagcgaagcg
    29881 gagctcatcg gtcgcctccc ggtccgagag tggtgacccg cgagaccttc ccgggccgac
    29941 gggggagcgc gcccccactg cggaccattg tcgcgtgcgg ccgtggcggt cggcgccacc
    30001 ggagtgcgcc gggggtcagg acccccggac gaggacggcg acgaggaagt cggaccccgg
    30061 tccgaacgga cgcaggtccc aggtcgccag gcgcagctcg acggccaggc cgacggcgtc
    30121 gacgtcggcg aagaactcgt cgaagtcgta gtcgcgtccc gctccgaacc cgacgaccag
    30181 acggccgtcc gcggccaggt gtgctcccat ccgccgcagc acctcacgcc gggtggccgg
    30241 gtcgaggaac gtcatgacgt tgccggcgtt gacgatcagg tcgaacgggt ccaggtcgcc
    30301 caggtcgagc tcgctcaggt caccgacgcg ccacgtcgcg cccggatgca ccgagcgggc
    30361 ctcgtcgatc agggccgggt ccaggtcgac gcccaccacg gtgtggccga gcaccgcaag
    30421 gcgtccgccg acccgacccg gaccgcaacc ggcgtcgagg atccgggcgc cgcggccggc
    30481 catggcgtcg atcagtcggg cctcaccgtc gaggtcccgg ccttcggccg ccatcgcctt
    30541 gaagcgctcg atgtaccagt cggagtgccc ggggttggcc tcgagcatct gcaaccatcg
    30601 gttctcgctc atcgaaccga cggtagcccc cggctccgcg gacgggcccg ggaccttggt
    30661 cacctccgac ggccggtggg ggaggggtcg gactcctcgg cgatgcgccg cttggtggcc
    30721 gcccacgagc agatcggaca cgtggtgagg tcgtggtggc gggccgggca gtgctcccgg
    30781 ccgaagtaga tgatctggag gtggcggcgg ttccacgtgt cgatcgggaa caccgccttg
    30841 aggtcccgtt cggtgcgttc gacgttcgtg gcgctcgaca gcccccagcg tccggccagg
    30901 cggtggatgt gggtgtcgac ggggaacgcc ggcacgccga acgcctggga catgaccacg
    30961 ctggcggtct tgtggccgac cccggggaga gattcgagga agtcccagtc ctcgatcacc
    31021 tgtccgccgt tctcgacgat gcgatccgcg gccagccaca ggttccgggc cttggtgggc
    31081 gccaggccga tctcgcggat cagctccagg atgcggtccg gcccgagcgc gaccatgtcc
    31141 tcgggcgtgg acgcctcggc gaacagcgcc ggggtgacct cgttgacctt cttgtcggtg
    31201 gtctgtgcgg acagcgcgac ggccaccagc agggtgtacg gatcggtgtg gtcgaggggg
    31261 atgggcgggt cggggtagag gtcgtcgagg atctccccga tgcggtcggc cttgtcctgg
    31321 cgcttcacgc cggcgaccct accggccccc accgaaccgc cgttctgtag tgcgagatcc
    31381 aggcgttcgc ctggatctcg cactacagaa cgggtcggcg gagggcatgc gccgcgacgg
    31441 cggggggagc cgataagaat gaaccccgct ccggcgccca cccccgggcg tcgacccaac
    31501 cgacagcgag ggacaacatg aagcgtgtaa cggctctgat cgccgcactg gccgtgccgg
    31561 cgatcctcct ggtcggctgc ggcggcgacg actccgccga cagcggctcc aagtccgatt
    31621 ccagcagcca gtcctcggac tcgggatcct cggactcggg ttcgtcggac tcgggcagct
    31681 cgtcgtcgac cggcaacgcc aaggtcgaca agttctgcga cgacgccaag gcgctggccg
    31741 agaagtccaa gaaggcgatc gccgatcgtg acaccgacct ggcccaggag gccgccaagg
    31801 acgcccaggc cctcagtcag caggcccagg acctgacctc cgaggtgatc aaggacccga
    31861 agctcgccac ggcgctgacc gactgcacca aggagctcgc gaacgtcggt cagggctgat
    31921 ccctgatcga catccggatc cggccggcgg gtgagtcccg ccggccgttc cgcgtccggg
    31981 cacctgacgg gagtcgagga cctgcactca gggcgcgggc agtcgctggg cgctgaggaa
    32041 gtcccagatc agctggctgg cgggcagcgc catgttggtc gggccgacca ccatctcgat
    32101 cgtttcgctg aaggcgctgc cgggccagga gtgcccgccg ccgtgcacca cgtagaactc
    32161 gacctctccg ccgggagggc agtcgtaggt gcgcagggtc acgtcgggga ccggttcggt
    32221 gtcggcgggc ttcgggcggc acccgttgtg ggcggcccac ttccggaccg cctccgggta
    32281 gcccggtccg tcgatgtcga ccccggcgat ggagctcgac gaggccgagc cgtcggggcc
    32341 acctccggag ctctcctccg gcgggtcctc accggcgagg aaccctccga ccccgccgtt
    32401 gaactgcagg atcgggtccg cggtgccgtg cacggccagc accgggaccg gtcggtcggt
    32461 ctggcagccg tcggggtact gcacgccggc caccggtgcg atggcagcga tgcgttcggc
    32521 gcgcacgcag cccagcagcg acgagaagat ggctccgttg gacaggccgg tggagtacac
    32581 ccgtgacatg tccaggcaca gctcggagcc gagcgcgtcg atcagctgat cggtgaagtc
    32641 caggtcgggg ttgtcctcgg tctcgaccgc ccagtgcacc ggcgtcccgg tgccgttggg
    32701 gtaggccacc acgaaccgct cccgcgcggc gatctcgtcg aagccgctca tccgggtgtg
    32761 gacctcggcg ccttcggcca ggccgtggaa gtcgagcagc agcggcagcg gatccggttc
    32821 ggcggcaccg gaggtggagg tggaggtgga ggtggaggag gatgtcgcgg cgggtcccgg
    32881 cgtggtcagc aggtaccagc gggtggtgcc gtcgacgtcg atctcgcgcc tctggtcggt
    32941 caccgcgggc gcctccccac ggttgcggca gcccggggag ggcaccggag cgatcggggc
    33001 ctcggtcgtc gtggtgctcg cggtcgtcgg ggccgcggtg gacgcgcccg acaccgacgg
    33061 ccagggtgac gagtcgtcgt cgctgctgca cgcgacggca cccacgacgg ccagcacggc
    33121 aacggtggcc atggcgacga acgctgcgaa ccgggtggtg cgccggggtc gggcccggac
    33181 cttttggggc ggtgtggtca tcggttccgg cacccccggg acggtacttc tccaccggcg
    33241 ccgcccgcgt ccgggaccgg cgtgaccttc accgctgtcg gcggcgccac agatacgtcg
    33301 agttcaggta ccagttgacg atgagcgcga cgaccgcacc gatcaggttc tgggcgacac
    33361 gggaccagtc ggcacccagc accgagagca ggaacccgat gctctggagg tacttgaaca
    33421 ccgccacgtg caccagggtc ccgatgagcg acatcaggaa gaacaccccg aacgcggggg
    33481 ccagcttcca gccccggtag cgctcctccc agaacgtgaa ctcgttgttg agcacgaaca
    33541 ggaccaggat cgacacgatg acgctcaggg cgaaggaccc ctcgatcggg tcgatggcgt
    33601 cgttgaggcc ggtggtgatc gtcggcactc cggcggcctc ggcgacggtg aacaccaggg
    33661 agttgacccc gaggccgacc aggccgacca gcacgtagag caggaagacg gggtcgacct
    33721 gtcggccgag tcggagctcg gcgacgccga gcaggtagga gcggatcacc gagccgttga
    33781 gcttggtctc gccgtgggtg cgggtccgga acgtgtagcc gacctcggcg acgcggaggt
    33841 cccggttgcg cccgatgaac tcgagcagga tcttgaaccc gcgggggttg atgctcggcg
    33901 cggcgcgggc gtaggcggac tcggagatca tgaagaaccc gctcatcggg tccgacaccg
    33961 gcacccgcag caggacccgg gcgatcagcg tggcgaccca ggacacgaac cgccgcgacg
    34021 ccgaccagtc gccgtagccg ccgccctcgg tggcccggga cccgacgcag acgtccgccc
    34081 cggagcggag ctcgtcgagc atggcgggca gggcgctggg gtcgtgttgc aggtcggcgt
    34141 cgatcaccgc caggtactcg ccgcgggcga tcgacatgcc gtcgagcacg gcggccgaca
    34201 ggcccttgtc gtggaagcgc cgcatgaccc gtacggaggg gtcggtgcgg gcgatctcct
    34261 cggcgacccg ccaggtcccg tcggggctgt cgtcgtcggc gacgacgatc tcgtggtcga
    34321 tgccgtccag ggcggcgtgg agctcctcga cgaggatcgg caggttctcg gactcgttga
    34381 aggtgggggt caccaccgac agcagcggcg cgctggccgg agcgtctccg aggtcgtcca
    34441 tggcgtccgc acgctaccgg cccctgtcgc agctgccgcg taccctgatc gccgtgtgcg
    34501 gtcgcttcgt gtcctcatct ccccccgacg agatcgcccg gtacttcgac gtcgacgaga
    34561 cctccgagca ggcactcgag caccgggcca acttcaacac ggcgccgacc accgacgtgt
    34621 tcgtggtgta ccgcgacggc tcgacccgtc ggctcgacac cttccactgg gggctcgtgc
    34681 cgcgctgggc caaggacctg tcgatcggca gccgcatgat caacgcccgg gccgagacgg
    34741 tggcgaccaa gccggcgttc cgccacgcct tcgcccgcaa gcgctgcatc ctgcccgtcg
    34801 acggcttcta cgagtggaag gcgccgccgc cgggccagaa gcgcaagcag ccgtacttca
    34861 tccaccgccc cgacggcgag ccgtacgcct tcgcggggct gtgggagcag tggaagggcc
    34921 ccggtcccga cggggacgag gtgacggttc gctcgacgac gatcatcacc ggcgccgcga
    34981 acacggcgat gagcgccatc cacgaccgga tgccgatcat cctggcccct tcggattggg
    35041 agtcgtggct gtcgccggag aacaccgaca ccgagatgct cggacggctg ctcgtgccgg
    35101 cccccgaccg cctgatcacc atgcacccgg tgtcgacaga cgtgaacaac gtccgcaaca
    35161 agggtgagca cctcgctgac gaggtcgacc cggtcctcga cgacggccgg ctcctctgag
    35221 ccggatcggc gatcggatcg ggggtcagcc ggggacgtgg gcgccgtgac cggcggcgcg
    35281 cagggcgccg cggatcctct cggcggcggc gtcgagcgcg gccgggtccg ggtcggggtc
    35341 gacgttggcg aagctcaggt cgctctcggc gtcgatcggg atcaggtgca cgtggcagtg
    35401 cggcacctcg agcccggcga tgatcgaccc gacccgcacc ggtgagaaag cgtccatcag
    35461 cgcccgtccg accgcctgct gcacctcgcc gatgcgggcc cacagctccg cggggacgtc
    35521 ggtccagtgg tcgatctgct cgaccggcac gacgagggtg tgacccggcc ggatcgggcc
    35581 gatggtcagg aacgccacga cgtcgtcgtc gcgccacacg aagcggccgg ggatgtcgcc
    35641 gcggatgatc atggtgaaca ccgacggggt gggctgctcg gtcatggacc gagactaggc
    35701 actgccgcgc cctacgatgc cgaccatgtc cgacacgacc gcgggggtgg gcccggcagc
    35761 cgagaccggc ggtggcgggg ccccgggtgt cgagccgtcg ggggatcgac tggcgaccct
    35821 gcccaacctg atcacgctgg tgcggctgct gtgcatccct gtgttcctgt acctgctgtt
    35881 cgtccgcgac gcccgcctgg gggccgcgat cctgctcggc gtgctcggcg ccaccgactg
    35941 ggtcgacggc tacgcggcgc ggcacctgaa ccaggtgtcg aacttcggca aggcgttcga
    36001 cccgaccgtc gaccggttgc tgctgatcgt cggggtggtg tcgatcatcg tggtcgacgg
    36061 ctgcccgctc tggttcgggc tggtcgtcgt ggcccgcgag gtgatcctgt cgatctgggt
    36121 ggtgtcgatc atggcgctcg gcgccaagcg catggacgtg acctggtggg gcaaggcggg
    36181 gaccttcggc atcatgatcg ccttcccggc gttcctggcc gccagcgacg agggcatccc
    36241 cgaggcctgg tcgagcttct tcgagctggt ggcctggtgc gcggcggtgc cgggcctggt
    36301 gttcgccatg atcgccttcg tcggttactt ccccgccggg gtccgggccc tgcgcgaagg
    36361 tcgggccgcc cgccaccggg ccgcggccgg cgtctgagcc cgcctgcgct ccgaacccgc
    36421 cgaaccgtgc cggacgcggc ggtccgggcg atagcgtcat ggccaaccga ccgtccctcg
    36481 ccggacggtc ccgcgaagga cggtgaggtc ccgtgaaggc cgtgatcatg gcaggcggtg
    36541 agggcacccg actgcgcccc ctgacatcca acagccccaa gccgatgctc ccgctggtca
    36601 accggccgat gatggagcac atcatcgacc tgctggtccg ccacggcatc gacgacatcg
    36661 tcgtgaccgt ggccttcatg gccaacaaca tccgcaacta cttcggcgac ggctcggagt
    36721 tcggcgtgaa gatctcgtac gcgtccgagg agacgccgct cggtaccgcc gggtcggtgc
    36781 gcaacgccat ggacgagctc gacgacacgt tcctggtcat ctccggcgac gtgctcaccg
    36841 acgtcgacct cggggcggtc atccagaccc accgcgagca ggaggccctg gcgaccatcg
    36901 gtctggtccg ggtcgagaac ccgctcgagt acggcatcgt catcacccgc gacgacggca
    36961 ccatcgaacg attcctggag aagccgacct ggggccaggt gttctccgac acgatcaact
    37021 ccggcatcta cgttctcgag cccaaggtgt tcgactacat cgccgcggac atctcggtcg
    37081 acttctccgg cgatgtgttc ccggcgctgc tcgacgacgg cgagaagctc gtcggcgcgg
    37141 tgctcgacgg ctactgggag gacgtcggca ccctgcccgc gtacgtccgg gcccacaagg
    37201 acatcctcga cggccgggtg caggtgggca tcccgggctt cgagatgtcc gacggcgtct
    37261 tcatcgccga gggcgccgag gtgcaccccg acgcggtcct caccggcccc gcggtcgtcg
    37321 gcgacaactg cttcgtcgac gccggggcgc ggctggggga gtacacggtg ctgggccagg
    37381 gcgtgcgggt ccgctccggc gcggagctcg aacgcactgt cgtgcacgag aacgcctacc
    37441 tgggcgagag cgtcaagctg cgcggcaccg tggtgggccg cgccgcggac ctgcgtcgca
    37501 acgcccgttg cgaggagggc gtggtgctcg gcgacgaggt cttcgtcggg gaggacgccg
    37561 tcatctcgac cgacgtcaag gtgtacccgt tcaagaccgt cgagggcgga gcggtggtca
    37621 acacgtcgtt gatctgggag tcccgtggcg cccgcagcct gttcggccgt ggcggcgtca
    37681 ccggcctggc caacgtcgac atcacctcgg agctggcggc caaggtggcg ctcgcgtacg
    37741 ccacgtcgct caagcgcaac accacggtgg tggtgtcacg cgactccagc cgctcggcgc
    37801 gcatgctcaa gcgggcgatg atgtcgggac tgaacgcggg tggcgtcaac gtcatggacc
    37861 tcgaggtcgc cagcgtcccg ctgacccggt tccactgccg cggtgtcgcc gtcggaggcg
    37921 cggtgtcgct gcgcctcagc cccgaggacc ccgacgcggt cgtcatccgg ttcttcgaca
    37981 ccgacggggt cgacatcacc gaggagcagc agcgcaagat cgaacggctc ttcggccggg
    38041 aggacttccg tcgggtgcgc ccggccgaca tcggcgacat cgacctggtg ccccgggcgc
    38101 tcgagcagta cgcggtcgcg ctcgagcaga ccatcgacgt caaggccgtc gcggatcggc
    38161 gcttcaaggt cgtgatcgat tattcctacg gctcgaccag cttcgccatg cccaacgtgc
    38221 tgtccaagct cagcgccgag gtgctggccc tcaacccgtt cgcgtcgacg agcggcgcgc
    38281 tgtccttcga gcgggaccgt cacgcggcgg gggtggccga gttggtccgg gcctccggag
    38341 cggacctcgg cgccgtgctc gacccgtcgg gagagacgct ggcgctggtc gacgacaccg
    38401 gcacggtcct caccgacgac gagatgctgt tctgcttcct cgacctggtc tgcgatcggc
    38461 tgctcggcga ctcggtcgcg ctgccggtca ccgccccgcg tgccgccgcg cagatcgtgg
    38521 ccgaccgggg gtaccacgtc cggtggacca agacgtcgac cgcggcgctg atggaggccg
    38581 cgtcggagcc cggggtgggc ttcggtgcca accgcgaggg cggggtgatc ctgcccgggt
    38641 tcctccccgc gttcgacgcg gcgggaggac tgctcaagat gctcgacctg ctcgcggcgc
    38701 gccaggtgca gctgtcggag gtccgggccg cggcccccgt cgcccacctg ctgcacgagg
    38761 cggtggtgac cccgtgggag cagaagggca ccgtcatgcg cagcctggtc gagcagaccc
    38821 acgggcgcga cgtcgacctg atcgacggga tcaaggtgca ccacgccacc gggtgggtgc
    38881 tggtgctgcc cgatcccgag gagccgatca cccacctgtg ggtcgagggt gacaccctcg
    38941 ccgaagcccg caccctcgtc caggagtacg cccggcgcat ccgccagatg ctgcgctgag
    39001 cgggacgggc gggtgccgcc gtcgggtagc gtgcggccca tgaacgtgcc cgacgacctc
    39061 tactactcct ccgaccacga gtggatccgg gtcgaggacg gcaggttccg catcggcatc
    39121 accgactacg cccaggacgc gctcggcgac gtggtcttcg tgcagtcccc cgacgtgggc
    39181 gccgagttcg gcgcgggcga caccttcggc gaggtcgaat ccaccaagtc cgtgtccgag
    39241 gtctacgccc cggtccaggg cgtcgtcgtc gaggtcaacg gcgacctgga ggtgtccccg
    39301 gagctggtca acagcgaccc ctacggcgcg ggctggctga tcgtcgtcga gcccgtcgac
    39361 ctccgttcgg tcgagaccct gctcgacgcc gacgcgtacc ggaagctcac cgaggcgtga
    39421 acgccgccgg ccccgacgag acgaccgacg gaccgctctg cccggtctgc agcagcgtca
    39481 acgcggccgg cgcgaacttc tgctcggcgt gcggcaacga gctccccgag gccgacgaca
    39541 gcgcgaccgg tacctacccg gtgctcggcg tcgacgtccc cgacgcaggc gaggtcggtc
    39601 agctcgtcgt catccggggc cccaccgcgg gttcccgcta cgccctcgac gaggacgtcg
    39661 tgacgatcgg acgccacccc gacagcacga tcttcctcga cgacgtcacc gtgtcacgcc
    39721 gacacgccga ggtccgacga gtcgagggcg ggcggttcat gctggccgac gtcggctcgc
    39781 tcaacggcac ctacctcgac ggggaccgcg tcgaggaggc ggtgttgcgg gaaggcgcac
    39841 aggtccaggt gggcaagtac cgtcttgtgt tcgtgatcgg caccctggga ggaggagcgt
    39901 gaccgcggat gggctcacct catcggtcca caccccggtc gacgccgcgg cggccactgc
    39961 ggtgcgcccc gcgcaccggc cgatcggcga ggtgctgtcg ctgctccagg aggagttccc
    40021 cgacgtcacg atctccaaga tccgcttcct cgagagccag ggcctcatcc agccggagcg
    40081 gaccgcgtcg ggctaccgcc ggttctccgc gtcggacgtc gaccgcctcg agtggatcct
    40141 gcgccagcag cgcgaccact tcctcccgtt gaaggtcatc cgcaagatgc tcgacgaggg
    40201 cgtcgaccgc tacgacccgg gcggaggatc ccagccgacc ctgttcaccg cggcgatcga
    40261 cgcgtcggtg ggtgaggacg acgagcccga ggcctccgac gagggtgccg gtccggatcc
    40321 cgatgccggg accggtacgc cgccgcgatc accggcgcac cccgcggtcg cgtcggcccc
    40381 gcctcgccct ccgacaccgt cgcgccagcc accgcggccc gccgagcgtg ccccggtccc
    40441 ggccggtgct gatccggtcg gcgccgtgcg ctcgtcggcg ccggccactg ctgccggcgg
    40501 tgggggatcc ccgacgccgt cggccccgac accgtcggat ccgtcgagcc gtcccgcacc
    40561 ggcgcgctcg gcgacggccg agggcgccgg atccgagccg cccgaaccgg cgcgtccgcc
    40621 gcacgagacc ccggccgacg tcgtggccgc cctccaggag gacccgcgac cggcgtcgcg
    40681 ggccgagccc cgtggcgccc gaacgtcgga ccgtccggtg cgcacgcgtc cgtcccgcga
    40741 gccggtggcg gccgacaccg cgctcggcgc cggggagctg tgcgcccagg tcggcatcag
    40801 cgaagccctg ctcgccgacc tcgagaggtt cggcctggtc cagtccgtcc cccgcggggg
    40861 atctccggtc tacggccctg ccgcgctcga ggtcgcccgc ctggccagcc gctacgccga
    40921 actgggcgtc gagccccgtc acctgcgcat gtacctggtc gccgccgagc gcgaggccgg
    40981 catggtcgag cagctggcgg ttcccctgct caagcagcgc aacccccagg cccagcagaa
    41041 cgccgcggcc ctggccggcg agctggcgga gctgggcgcc gagctgcacc gatcgctgct
    41101 gcggcgcgag gtgggcagcg acctgctgcc gtgagccccg ggagccacca ctggcatcgg
    41161 accggctgac gtaagctgcc gcggtgatcg aactggagtt ggtcgcggtg cgcgtcgagc
    41221 tgccgagcaa cacgccggtg ctggtcctgc gcgagctcgt cggccgccgc cggcagctga
    41281 cgatcttcat cggcaacccc gaggcgaccg ccatcgcgtt cgcactcgag ggcgtcgaca
    41341 cgccccggcc gctcacccac gacctgttct gcgacgtcct cgacggcctc ggcaccagcc
    41401 tggaacgggt cgtcatcacc gagctgcgcg agaccaccta ctacgcggac ctgcacctga
    41461 gctcggagga cggtccggtg acggtgtcga gtcgcccctc cgacgcggtg gcgctggcgg
    41521 tgcgcaccgg ctgccccatc ttctccgagg agaccgtcct cgacgaggcc ggcttcgtcg
    41581 aggaggtcga cgacgaggac gtcgacgacg acgaggtggt cgaggagttc cgccacttca
    41641 tcgacaacgt ctccccggac gacttcgcca gctgagctgg gagtgcggtc cacccacccg
    41701 cgggcatccc ggcgataggg tgaatcacat gactgtcatg cccggcgact ccgcctccga
    41761 cgtctcctac agcggccgca gcgccgccga gatcgtcggc atcagctacc gccagctcga
    41821 ctactgggcc cgcaccgacc tggtgcgacc gtcggtcacc gacgcggcgg gcagcggcag
    41881 ccgccgccgc tactcctact cggacctgct cgagctgaag gtgatcaagt ccctgctcga
    41941 cgccggcatc cgactcgagt cggtccgcca ggtcttcgac tacgtgcgcg agcacctcgg
    42001 cgaggacatc ggctcggcca acatcgtcat cagcgggacg tcgaccgtgc tggtccgctc
    42061 gggcgacgag ctgatcgacg tgctgcgcaa gggccagggc gtgctgaacg tgctcgccct
    42121 ctcgggcgtc aaggacgagg tcgacgcccg catcgtcgag ctgttccccg tcgccaccgc
    42181 cgaggaccgg acgcaccacc ccagcgctgc cggcgccggc gcgggctgag ccgggcggtg
    42241 cgcacctcgc cgctcgacgg cgtgcaccgc tccctgcggg cgcggatggt cgagttcggg
    42301 ggctgggaca tgcccgtgtc ctacccgtcg gggaccctcg aggagcaccg gctgtgccgt
    42361 cagaccgcgg tcatgttcga cgtgagccac ctcggcaccg tgcgggtcga gggcccgggc
    42421 gcgttcgact cgttgcagcg tgagctgacc aacgacctga caaagatcgg tccgggtcgc
    42481 gcccagtaca cccacctgct cgaccccgac gacgcgtcgg tgctcgacga catcatcgtc
    42541 tggtggctgc ccgacaccga cggcgtcgac cgtttcgacg tcatgcccaa cgcgtcgaac
    42601 accgaccggg tggtcgcagc gttcgccgcg gacggggtcg acgtcgtcga caccacctcc
    42661 ggacgagccg tgctcgccgt gcagggaccg acgtcgcgtc aggtcgtggc gacggtctcc
    42721 gccgaggccg ccgcggtcgg acgcttccgg gtgatcgagg ccgacgtcgc cgggcaccgc
    42781 tgcctggtgg ccggaacc
//
LOCUS       DAOQOD010000006        41366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_286_length_41366_cov_11.655273, whole genome shotgun sequence.
ACCESSION   DAOQOD010000006 DAOQOD010000000
VERSION     DAOQOD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 41366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41366
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_41366_cov_11.655273"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLZ72_01580"
     CDS             complement(<1..500)
                     /locus_tag="PLZ72_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35014.1"
                     /translation="MRRPATRTQIVAVSAIGVVAGSLAALTSDAAPTGTDLFDVVWRV
                     ALVVVSTIAGARARRWSLAAAAGLVVVGAAGRWSVAGLIALGLCFALAWEDRRSRIAG
                     AASAALIALAALHLGWPTPTFATAALASVALGMVWVSAYRTSSRRIRRRVRISVLAVG
                     AFALVGT"
     gene            671..1132
                     /gene="rbfA"
                     /locus_tag="PLZ72_01585"
     CDS             671..1132
                     /gene="rbfA"
                     /locus_tag="PLZ72_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNA35015.1"
                     /translation="MGMARRRRTAARRAPRQYSRTDRLGELVREVVASELERIGDERL
                     ELVTITDATVDGSLEHADVFWSALQAEDDGRLDEVVEALEEQRWKVQQVVNREVRARK
                     TPQISFRHDDVLRSALRVDQILRDIEPGATGAPEPDGGSDGEPDGDPDDGS"
     gene            1129..2040
                     /gene="truB"
                     /locus_tag="PLZ72_01590"
     CDS             1129..2040
                     /gene="truB"
                     /locus_tag="PLZ72_01590"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNA35016.1"
                     /translation="MNADGNPDAPLGLCVVDKPSGWTSHDVVGRCRRIFGTRKIGHSG
                     TLDPMATGVLVLGVGRATRLLTFLGGLPKTYEAVIRMGVETDSLDADGEVTVTHRMDA
                     PDLEAVRAAARQLTGDLQQVPPMVSAKKVDGRRLHELARRGEVVERAPVPVTVTRFDV
                     EATDDPMVVGAVVSCSAGTYVRVLASDLGHALGGGAHLVGLRRTAVGPFDLSDAATLE
                     QLEADPSAHLLAPTAIERVMSCVVADPAVAGEVLHGRVLGRDRLGLGDDGDDTGPWAV
                     LDPDRSRLLAVYAPHREGTVKPAFVLS"
     gene            2093..3076
                     /locus_tag="PLZ72_01595"
     CDS             2093..3076
                     /locus_tag="PLZ72_01595"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNA35017.1"
                     /translation="MEVHRLNQVRSAGASTTCPRPAEGSVVTIGAYDGVHLGHRAVIG
                     DVRRRAAAGGHASAVVTFDRHPAQVIRPESAPLLLTDLDQKLELLAATGVDHTLVVGF
                     DRERAAESAEDFVRTVLVDCLNVRQVVVGEDFHFGHRRAGNVALLEELGGPLGFGVHG
                     HELVGADGRAARDETQVSSTAIRRALVEGRLADANAMLGRPHEMRGPVTRGDARGRTL
                     GFPTANVAIPAEMLMPADGIYAGELELSDTGTVLPSAIYVGKRPTFYDDRAMTLLEVH
                     ALDFAGDIYDRATSVRFTHRIRPDARFASVEELTTQLRLDCDEAARLLGLR"
     gene            3145..4365
                     /locus_tag="PLZ72_01600"
     CDS             3145..4365
                     /locus_tag="PLZ72_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35018.1"
                     /translation="MSHIESSEIVDPIEGPVRDEPVAPGAVEVPRLARHEITLADGHL
                     VGVTVAGEGIPLVVVHGFSAEGFLYAQSLSRLVGMGFRVIAIDTAGHGATQGLPLSGQ
                     AMNDYAELLGRCIDELGIERFVLAGHSMGGQLVTRLAAQWPERTMAVILIDAIVGDTW
                     DRMVYLFRVAPPLLGMVGALLVVDSLTIPPAFSDPRQATKLMRLAVPTVAGHLVQPWR
                     LMGPMMSILRTRSSRYALDAIREGEIPVIALHGSRDIAVPHRTSVDAVRRTEGTLVTI
                     QRGGHSWPLTDPETLPAIMAEMLDGPLGDGIRRSLRAAGVRTRTPSIDQIRSACYDPD
                     ARVFDLDPVDARRKVVGRHRKPRYRWTVEHPRPTGSAPPTPNGRERTDRRSPTTAGGR
                     KARPAKLDVVDGDA"
     gene            4486..4767
                     /gene="rpsO"
                     /locus_tag="PLZ72_01605"
     CDS             4486..4767
                     /gene="rpsO"
                     /locus_tag="PLZ72_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNA35019.1"
                     /translation="MTNREPTNLPPKGDTIAKFRKHDTDTGSPEVQIALLTDRINHLT
                     EHLKVHKKDHHSRRGLLMLVGKRRRFLDYLKNNDVERYRTVIAELGLRR"
     gene            5110..7551
                     /locus_tag="PLZ72_01610"
     CDS             5110..7551
                     /locus_tag="PLZ72_01610"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNA35020.1"
                     /translation="MAEPTRVAAPIGGDDKTISFETGKLAGLAGGAVMAGMGNTRILV
                     TATGAKHVRDGIDFFPLTVDIEERMYAAGKIPGSFFRREGRAPESAILTCRLIDRPLR
                     PSFPDGFRNEVHIVGTVMGADQQNPYDVLAINAASAALMISDVPFEGPIGAVRLAYST
                     DGEWIAHPTYEESDEATFEIVVAGRQLDDGDVAVMMVEAGGTEKSFQYYDEGAPKVDE
                     AALATALQECKQYIKAVIALQQELVDAVGAKPTMSFDVQVDYTDDIYAQVKSLGGERM
                     NEIMQIADKSERGAAEDALRTEMVDRIVPELVTAAVAEGADEATAQAQATKQVKSAVR
                     SLSKNTVRTRIVNDGMRIDGRGLRDIRPLSSEVGVLPTAHGSGLFQRGETQVLNICTL
                     GMAKMDQMIDGIDPITRKRYLHHYNFPPFSTGETGFMRGPKRREIGHGALAERAVFPV
                     VPSLEEFPYTIRLVSEVLSSNGSTSMGSVCSSTLSLMDAGVPIKAPVAGIAMGLVHAD
                     GQYATLTDILGAEDAFGDMDFKVAGTADAVTALQLDTKIDGIPADVLAAALEQAKEAR
                     LQILDVMAGAIAEPRPEVNETAPKIISFEIPADKIGEVIGPKGKVINSIQSETGANIS
                     VDDDGLVGIVSIAAVDGGAVAEAERQIKLILDPPTAELGATYDGRVVNITKFGAFVNI
                     LPGRDGLVHISKLGGGKRIDKVEDVLTLGDAITVKVDDIDPNGKISLSPVGELAGGDN
                     PKASDGVGSADAVSAAGPDSEERSADRRELDFGAAFDAELREEFGDLGPAPVRSGGGG
                     DRGRGGRGRGGNRRR"
     gene            7590..8828
                     /locus_tag="PLZ72_01615"
     CDS             7590..8828
                     /locus_tag="PLZ72_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNA35021.1"
                     /translation="MLPSPDEQVRRTVLDNGLRVVTDRLPTSRSVAASVWVGVGGRDE
                     SDEMSGASHFLEHLLFKGTDERDAKSIAVAIDGIGGDMNAFTANEHTAFYARMPAVEA
                     HVGIDLLLDVVQNPALRDWEFDGEREVILEELAAAEDDPDDVSSVKLFERLFPDHPLG
                     REVLGSIESIDGLTRDAVRDFFGEWYTPANLVVAAAGDIDHDLLVDEVDRRFGDRSPG
                     PVPTRTEPDDRLGESSVEVRPIEVVHLAMGWRALAVDDDGRYALALLNHVFGSGPSSR
                     LFQEVREERGLTYSISSAVSHHVDTGAVSVHCATAPGKVDDLIAVVDTILDDVVSHGI
                     SREELARAKGSLRGAYLMSYEDVGTRMTRLGMSETMRGGLTPIDEHLARLEAVTDDEV
                     QRVAEQVLGSARVMSSVGPR"
     gene            complement(8875..10098)
                     /gene="serB"
                     /locus_tag="PLZ72_01620"
     CDS             complement(8875..10098)
                     /gene="serB"
                     /locus_tag="PLZ72_01620"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNA35022.1"
                     /translation="MPIAPDDPSPAPTAALLLRVSGPDHPGITADLMTLLDLAEVEVQ
                     DVEQVLVRGQLTLAVAVSDPGDLRDRLPQLLDPLAEGRGVRVELSSAPPPRPAHQRTE
                     AVTVLGHATETPLSPAEIAAVAGGINAAGGNIDRIVRLARYPVYAYEFRVTGAEPTAL
                     AQHLGEAAALHRLDIARQPAGLERRAKRLVVLDVDSTLIQDEVIELIAAEAGCLDEVR
                     AVTERAMSGELDFTAALTERVARLKGLEVDALDRARARMRLTPGARTFVRTLKRLGYT
                     VGIVSGGFTHFTDGLAAELGVDHARANQLEVHDGRLTGRVVGEVVDRGAKARFLRSFA
                     EAEGIPLSQTVAVGDGANDLDMLATAGLGVAFNAKPVVQEAADTTVNVPYLDAILFVL
                     GVTRDEVERADTDTG"
     gene            10146..10973
                     /locus_tag="PLZ72_01625"
     CDS             10146..10973
                     /locus_tag="PLZ72_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA35023.1"
                     /translation="MDVESHGEHVRIVRLDRPERLNALSIDLAVELDSALASVGADNS
                     ARVVILTGTGRAFCSGLDLKDYGVIPNIDGLTVHRIASRSMRTYSQITRRLRSIPQPV
                     IAAVNGPAYGGGMCLALGADLRIADETASFNATGIVNGLTSAEMGAGWLLPRLIGAAN
                     ANDILLTGRRVDAAEAQRMGLVSRVVPAGTVLDEALEMAADMVRYSHHGLEATKQALW
                     VELEINSLDAAIEFEDRNQLMAGFTDNLPTAIRAFDRDEQPVYLDEPRRDLFEQPGG"
     gene            11096..11887
                     /gene="dapB"
                     /locus_tag="PLZ72_01630"
     CDS             11096..11887
                     /gene="dapB"
                     /locus_tag="PLZ72_01630"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNA35024.1"
                     /translation="MIRVGVLGAGGRMGATVCEAVHGDDDLSLVAAVDPSYSGLDLRT
                     VTGVDAGIRIASGPAALERGAVQVVVDFSHRAAAMDNLRWCAEHGVHAVVGTTGFTDD
                     DMAAFDAMFTRSNCVIAPNFAIGAVLMMHFAEVAAPYFDSAEIIELHHENKLDAPSGT
                     AVHTAQRMAEASKDWTPDPTTSELIEGARGGVGPADIHIHSVRLRGLVAHQEVLLGTA
                     GQTLTLRHDSIDRTSFMPGVVLAVKKVADRPGVTVGLEALLDLPG"
     gene            complement(11915..12610)
                     /locus_tag="PLZ72_01635"
     CDS             complement(11915..12610)
                     /locus_tag="PLZ72_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35025.1"
                     /translation="MSYAAPALPPDHQTHPRWLLPLLTAGIVLLVIASNLGNMVWADW
                     VQSRPLGLIALNSSNKYLLMTSISLDLAPMVVVASLRLLAPDPIFFAMGWLYGDRALH
                     WARRTFPGGTHLLDRVHEDPRAVHRVLNVLVVVAPNNLVCLVAGVVRFPLRRFIALNL
                     VGTVGRVLLMRWLGHLFEDQIEHVLDVVDRYQTWLLWGSVALVAALVVWQMLSDRGLV
                     GGLEELEDELGDD"
     gene            12773..14401
                     /locus_tag="PLZ72_01640"
     CDS             12773..14401
                     /locus_tag="PLZ72_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNA35026.1"
                     /translation="MQGTMQDRPLTLNHLFERAERLNPTKEVVTATATGVDRYTYGEW
                     ADRTRRLAAALDSLGISPDGRVATFAWNTGRHLELYFAAPCSGRVLHTLNIRLFPEQV
                     TYIVNHAEDEVIFVDRSLLGLLAPLMPTFRTVRHVVVMEDGGQGPDPEGVEVLDYEEL
                     IGAQAPAEFGVDDEYRAASMCYTSGTTGNPKGVVYTHRSTFLHTMGAMLADSVGVCES
                     DVVLPVVPMFHANAWGLAHAGVASGAKLVMPGPNMVPVKLAEMIESEDVTLAAGVPTI
                     WMGVLPELAGRDTSSLRAIPCGGSAVPKALSEAYRAQIGLPILQAWGMTETSPVASVA
                     NVTTVLEGLPEDELADVRASQGRPVLGVELRIADQATGEILPWDGQSRGELQAAGPWI
                     AAGYYDDPRSPESFTDDGWLKTGDVAVMTPEGYVRIVDRTKDVVKSGGEWISSVELEN
                     EIMAHPKVAEAAVIGVPHPKWAERPLACVVVKPGEELTKEEVVEFLDGRVAKWWLPDD
                     VVFIDEVPKTSVGKFSKKDLRDRFDDYTLPTAGD"
     gene            14490..15365
                     /locus_tag="PLZ72_01645"
     CDS             14490..15365
                     /locus_tag="PLZ72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNA35027.1"
                     /translation="MYDFLRRPAWIVSHVLVVIGVLAMIGLGFWQRQRWIDERVKSDA
                     LAAQAAGTPVPLDEALADSGVDASTAPAAVPESVEFRRVEVSGVYDAAGEVLVRNRSQ
                     GGAPGAWVLTPLVQADGTAVAVVRGWVPLDIDPPAPPFPGSEPPAGEVTVTGLVQRSQ
                     QHGTFGGTDPATGTLRTLARVDVPRLDQQVDAAFEPVYVMLDGQTPPQPASTDGMPEP
                     VKLELPTPETNFSYMVQWWIFALIAAAGYPLVLRRVAQNRVRGDQTPDDDPVDLPGSG
                     AADVPVATGNDEVPT"
     gene            15365..>15756
                     /locus_tag="PLZ72_01650"
     CDS             15365..>15756
                     /locus_tag="PLZ72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA35028.1"
                     /translation="MAEDGAAGPVDVRERIMFAAVRCVERGGVRDVSMEAVAAEAGLS
                     RTTIYRHFPGGRSQLVSETATWEVARFWRRLALAVADLETLEDRLVTGLVIGTKLIRR
                     SSIMANLLEPEIDELVNALQPSESIVHQV"
     assembly_gap    15757..15856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15857..16467
                     /locus_tag="PLZ72_01655"
     CDS             <15857..16467
                     /locus_tag="PLZ72_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35029.1"
                     /translation="GPSAPGVALGTYLEPDSAEARVVDALFDCVARWGMAKTTVEDVA
                     RSAGISRATVYRLFPGGKGAILQAGLGSEITRLVGELNRQLEPLATLEEVLARAVSFS
                     ITEMREHRAFGQVPEHEWASIEKHLTFDRLDPVFLLAGAAFGPSLMRFLTPEDASHVA
                     MWAARLVVSYLQSPSDDLDPGREDTARHLVVTYLLPGLEPAA"
     gene            16515..17654
                     /locus_tag="PLZ72_01660"
     CDS             16515..17654
                     /locus_tag="PLZ72_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA35030.1"
                     /translation="MSINEEILGRADVDDLEAILAVSNSDRDAVISTVQQNAEAIFTW
                     DYTKGARPALDKLYEKAKVSMWNGETDLPWDTEVDQESIVIANAEQNSGVNGAGALGL
                     ADFDVTGTPFEKWTPDQWMRLGIESQNWTLSQFMHGEQGALICTAKIVETVPWIDAKY
                     YASTQVMDEARHVEVFARYLDTKLSGHYPVNAHLKMLLDDIITDSRWDMTYLGMQVMV
                     EGLALASFGFMHQMSTEPLLKQLLRYVMSDEARHVAFGVLSLQEYYANLSDAEIFERQ
                     QFAFEAAVRMRDRFLQQEVWERMDVPVKDAIEAVMRTPTRQLFQIMLFSKIVPNCKKL
                     GLLDRNDAWLRRRFEEIGVIQFEDWEDTGSEYESFALTDADQRSA"
     gene            17805..18503
                     /locus_tag="PLZ72_01665"
     CDS             17805..18503
                     /locus_tag="PLZ72_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35031.1"
                     /translation="MSDDLATVLLQLRLRAFATTDVLRAATAVDGDRLDEVLRTAESD
                     RLVRHREGRIVGWSLTAAGRTRGQELLSAELDAAGTRDAVLDAYGAFLPLNAELLSIC
                     TDWQVVVVDGEHVPNDHSDPERDSSVLARLARLHPAAIEVTSALGRTVPRFTGYGPRL
                     IESHDHVLAGRTEWLTRVTGDSYHGVWFELHEHLLAVLGRDREHEATPDAIPTNAAGS
                     GRPGRRAPGTGDTP"
     gene            18500..19381
                     /gene="dapA"
                     /locus_tag="PLZ72_01670"
     CDS             18500..19381
                     /gene="dapA"
                     /locus_tag="PLZ72_01670"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNA35032.1"
                     /translation="MSATFGRVITAMVTPFDDRGELDLDAAVELANWLVENGSDGLVL
                     CGTTGESPTLTESEELVLFRTVRGAVSVPVIAGAGSNSTATAVEQTRRASELGVDAIL
                     SVTPYYNRPSQAGLDAHFRAVAAATDLPVILYDIPVRTGRKIDTDTMLGLAHDVPNIV
                     GVKDASGDPTETARLIASAPSAFEVYSGDDAQTLPLLAVGAVGVISVASHWVGRQMGE
                     MIDAFEKGDVSTARRINAGLLDSYDYESSPDAVNPVPTKTMLRVLGLKVGSCRPPMGP
                     APDDLEDRARRVLDGLA"
     gene            19381..21039
                     /locus_tag="PLZ72_01675"
     CDS             19381..21039
                     /locus_tag="PLZ72_01675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNA35033.1"
                     /translation="MAPPVHITFLGGLGEIGRNCAALELEGQILLIDCGLMFPDNDML
                     GVDLVLPDFTWLLERSDDIVGCIATHGHEDHVGGLSYLLREASFPIFGTELTLGLARG
                     RIEESGLMGRTDLCVAADGDTWEIGPFEVEFIPTTHSVPQAVATAFHTPQGTIVHSGD
                     FKIDLTPVDGRLTNLSRLGALADGEGIRLLLADSTNADQRGHSRSETEIGRVLHQLMY
                     EQRGRRVIVACFASHIHRIQQLADAAVDSGRVIAPLGRSMRRNISMAREMGLLRIPDS
                     ALIDVEDVDRHEPGKVLVLCTGSQGEPMSALTLLAGQENRWLKLHPDDTVILSSHPIP
                     GNEHAVTKVIDNLFRQGVEVIHSGVSDVHATGHAKAEELKLYQSILKPDWFVPVHGEY
                     HHLVAHARLAETMGTPHDHIVVAEDGDRLVLDDDGLREIGRVPAEYVYVHGTVGGVGT
                     GVLADRRILADEGVVSAIVCVDRKKKRIVAGPQITTRGWVYEEESADLLSDLAERVRA
                     AVDSGLAEDAEVSRLVKLVRKAAGSFVSDRTRRRPMIVPVVLEA"
     gene            21320..23686
                     /locus_tag="PLZ72_01680"
     CDS             21320..23686
                     /locus_tag="PLZ72_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA35034.1"
                     /translation="MFGRLGAWCCRRRWIVLAAWLVVLVAGGALTGALGSNFKSEFNL
                     PNVESKHGMDLLASDFGGIGAGSSGSIVFRTDGQFSDAQRAELEAFLDDVADVDDTTV
                     RSPFGPTGAPQISTRGDDGTIAYADIQVPVLLDQEGASEYAEEVRGLAPHVDGVDIAY
                     GGQLFVEFEPPDSELLGLAFAIVILITAFGSVLAMGLPIGVALGGIGVGSILLTLLSN
                     TVPMPDFATTLGVMIGLGVGIDYALFIVTRFREQLHLGHTVEESVAISLDTSGRAVTF
                     AGLTVVISLLGMLLMGVSFIQGLGIGAATVVAVTMVASLTLLPAMLGFAGERVERTRW
                     RGLIAAGLVAVALLGAGLKIQPLAAGAILVAAVVIVLGFFWTPLRREVPRREPAPLRT
                     TRAYRWSRVVQHHPWSMAIGATVFLVVLTIPAFGMHLGFPDEGNDPPGTTTREAYDLL
                     AEGFGPGHNGPLVLVSKLPEGTTPEQLAAVTTALEDTPGVESVSPALPNTLITGKDGD
                     PTAVMWRVVPTTAPQDQATTELVHTLRDEVLPRAQTGTGLDVLVSGFVAVTVDFTSFV
                     SERLVLFFAAVLSLSFLLLMVVFRSLLVPVKAVIMNLLSIGAAYGVLVAIFQWGWAKD
                     IFGVEPAPIAPFMPMMLFAIVFGLSMDYEVFLLSRIREEWVRTGDAKASVADGLAATA
                     RVITAAAAIMVFVFGSFLGESMRTVKLFGFGLALAVLLDATVVRMLLVPATMELLGDR
                     NWWLPRWLDRILPSVNIEGTLEHTNPGEDDDAEDDPTVPSSDRQPEPV"
     gene            23958..26225
                     /locus_tag="PLZ72_01685"
     CDS             23958..26225
                     /locus_tag="PLZ72_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNA35035.1"
                     /translation="MAGLLLILVGIVTALGVYAGAAGPLGSALASLSAGLVGRLDLVV
                     PPLLVAGGVLVVIGPRRADVDLTGESGDTASGRGAVDPHERHVARIRRGIGIGLAILT
                     LLGFLDLASGAGLTVEADGLDAFAAAGGLLGAAVAGVFEAVVGSGGAVAVLVVLAVLS
                     ASLMTGRPLRTVASDVGHAAAPLLRRIGVWFSDLFRVGSAEAADGDTADPAPDVALFD
                     QDRDAKPAPKRSRPRKPTTPDVVVAANQEPVDPTQLEIELPPGLAGSAWKLPPVKLLS
                     LSGRRDIDTRAVAARGDVLVSALAEHGVQTRIVGMTVGPTVTRYELELGTGVKVARVT
                     SLNKDIAYAMASPDVRILAPIPGRQAIGVEVPNADRVVVNLGDILTSEEARQATHPLQ
                     VAVGRDINGTAKLMNLATMPHLLIAGQTGAGKSSCINSIITSVMMRATPEQVRMILID
                     PKRVELTQYNRIPHLLTQVVANPKKAANALNWAVKEMERRYDLLSEVGVRDITGYNKK
                     FDKGELTPEPGSERTYDRLPFILIVVDELADLMMVAPRDVEESICRIAQMARAVGLHL
                     VIATQRPSVNVITGVIKANVPARLAFAVASATDSKVILDQGGAERLVGRGDMLLLEGG
                     SSAPERIQGAWVEESEVHSIVSSWRRQAPDVESQYVSDVQGSDDAGGSTSGGSGSGDD
                     DDLLAQAMELVVRSQLGSTSMLQRKLKVGFARAGRLMDLLEERGVVGPSEGSKARDVL
                     MSPEELDNLIESGAI"
     gene            26225..26566
                     /locus_tag="PLZ72_01690"
     CDS             26225..26566
                     /locus_tag="PLZ72_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNA35036.1"
                     /translation="MDPDDSGRCGGRHRDPDAFAEAVLRVVDRVPPGRVTTYGAVADA
                     VSDRFGGGPRQVGAVMARDGAAVAWWRVVRADGSLPDPLAAEARARHRQEGTPLRSSG
                     RVDLVAAFWEP"
     gene            26600..27067
                     /locus_tag="PLZ72_01695"
     CDS             26600..27067
                     /locus_tag="PLZ72_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HNA35037.1"
                     /translation="MPAKDYTAEAITVHWRSERCIHSARCLQGLPSVFDRDARPWIRP
                     DGATADRVAAVVDTCPSRALTYTRTDGSAGGHNAERPDEDAPSDASTPVVVNVKRNGP
                     MVVVGPVTVVDADGNVVSRDDRTFLCRCGGSARKPFCDGTHKSRGDWDDHTAR"
     gene            27183..28130
                     /locus_tag="PLZ72_01700"
     CDS             27183..28130
                     /locus_tag="PLZ72_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNA35038.1"
                     /translation="MTDATTVTTVPTEWTTLATGLRFPEGPVGLDDGSVLLVEIERGT
                     LSRVSPDGTVDVVADCGGGPNGAAIGPDGAVWITNNGGCFEFVDLGGMLVPGPVPPSW
                     SGGCIQKVDPTSGSVQTVYTECDGRPLRAPNDLVFDRHGGFWFTDHGVRLERSSDRTG
                     VFYAAADGSSIREVLFPLDAPNGVGLSPAGDRLYVAETHTGRVWAWDVPGPGVAQGSG
                     LLEPHGDLLCGLPGHQLLDSLAVDGDGWVCVGTLLNGGITAISPDGSRLEHHPADDLM
                     VTNICFGGPEGRTAYATASSSGRLLTAEWPRPGLRLAYP"
     gene            28165..28806
                     /locus_tag="PLZ72_01705"
     CDS             28165..28806
                     /locus_tag="PLZ72_01705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35039.1"
                     /translation="MTDSWTDEAKVREYTGRVGRLAARRAGEMELVEALPAEVERVLD
                     LGCGDGRLISLVLDARPGVAEAVGLDSSPPMLERARDAFDGDPRVRIVAHDLRHPLPD
                     LGRFDVVVSGFAIHHLDHDRKRSLFSEVVAAIRPGGVFANLEVVRCATPELHEEFNRR
                     IGRPGGDPEDVLAAVEPQLEWMRVAGLEHVDCNWRWRGFALLVGRAPGGTPDR"
     gene            28870..29448
                     /locus_tag="PLZ72_01710"
     CDS             28870..29448
                     /locus_tag="PLZ72_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA35040.1"
                     /translation="MTLLARLWLALWRWSVAPAAEPVPDRCVMIAAPHTSNWDFPLTL
                     ALARVSGVKISWLGKKSLFRGPAGPIMRRLGGVPIDRSAPGGMVTSLANEFEGRDRLV
                     LVVPAEGTRSRTEHWKSGFYRIAQQADVPIVCAFVDRRTRTGGFGPVISPSDDLVADM
                     DRIRDFYSGMEGLKPGRFGEIRLRDEDALPAG"
     gene            29521..30795
                     /gene="rimO"
                     /locus_tag="PLZ72_01715"
     CDS             29521..30795
                     /gene="rimO"
                     /locus_tag="PLZ72_01715"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNA35041.1"
                     /translation="MSRTYWVETLGCPKNQVDSDKLIGTLHADGLLAAASADEADLVV
                     VNTCAFVAEAREESVATILALEERRAEGAELVVTGCMAERYGDELAAALPEVDAVAGF
                     GVPVTLGPTRGSGPAVPTMDLLNLPRPRSERPWAYVKVAEGCDKACGFCAIPSFRGPQ
                     RSRPIEAVLDEVAALQVREVVLVAQDLAAYGRDRDTDGRIDVASGTTPIAELVRAVAA
                     EVDRVRLLYLYPSEMTDELVELIATGPVPYFDLSLQHVSAPLVRRMRRWGDGERFLAR
                     IDAIRTLRPDAAFRSNFIVGYPGETEDDHDQLLDFVESARLDWCGFFAYSAEDGTYAA
                     GLDGAVEPALVGDRLAELRELQDTITGRARDALVGERTVVLVDEVGVARSHREAPEID
                     GIIEVPDTLAVGELHLVEITAAAGPDLTAEPV"
     gene            30792..31382
                     /locus_tag="PLZ72_01720"
     CDS             30792..31382
                     /locus_tag="PLZ72_01720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNA35042.1"
                     /translation="MSTHPPDSTERSRTFGPSALLTPANVITVVRLLLAPVAFVLIYE
                     QRSGWLLFGLWIAITASDSLDGYLARRQGTTRSGAFLDPLADKVLALGGLWAMVLAGR
                     FWWLPVLLITVREALISAFRSYWGRRGLAVPASNVAKFKTFLQFSSVSLVVWPWTTGY
                     TWMADTVLWLAVAVAWISAAQYVQAGSRATTTMTGD"
     gene            31434..32741
                     /locus_tag="PLZ72_01725"
     CDS             31434..32741
                     /locus_tag="PLZ72_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNA35043.1"
                     /translation="MRVEVVAVGTELLLGQIVDSNSSWIGEQLALAGLDSYFQTKVGD
                     NLTRIAEVIELALSRSDAVICTGGLGPTQDDLTREAIARVMGVELEESEEAAERIIAM
                     FGGRGRRMSMNNLRQAMKPVGAEFIPEQPGTAPGLICPLERPDPLTGEAVPKVIYAVP
                     GVPWEMQEMVSGTVLADLQRRAGITSSIASRTLRTWGESESGLAEMLAGRMDELDRLA
                     DSGASAATIAFLASGGEGLKVRLTAKAATAEGVADILAAEEQVVRGLLGGLVFGVDDD
                     TMESVVLDLLRERGMTLGVAESLTGGMVGSRLCDVPGASDVFRGSVVSYASDVKFELL
                     GVPEGPVVTEDAARAMALGARRVLRTDVGIAVTGVAGPERQEDRPAGTVCLAVAIGEP
                     GSAGQGECLLDSIEVRLPGRRRQVREFSVISLLGLLRRHLLEI"
     gene            32962..34026
                     /gene="recA"
                     /locus_tag="PLZ72_01730"
     CDS             32962..34026
                     /gene="recA"
                     /locus_tag="PLZ72_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNA35044.1"
                     /translation="MEREKALEMALGQIEKQFGKGSVMKMGDKGTMAVESIPTGALAL
                     DIALGIGGLPRGRVVEIYGPESSGKSTLAMHVVAEAQRNGGVCAYVDAEHAMDPVYAA
                     KIGVDVDELLISQPDTGEQALEISDMLVRSGAIDVLVVDSVAALTPRAEIEGEMGDSH
                     VGLQARLMSQALRKLTANLNKTQTICIFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIESIKDGVEVVGNRTRVKIVKNKVAPPFRQAEFDIMYGKGISREGSLLDIGV
                     EHDIVKKSGAWYTYEGEQLGQGRENAKAFLTEHPEVMVEIQDRVLRAVGIGQGESEES
                     ESTDLSDDVVDPDDLPITLD"
     gene            complement(34279..34935)
                     /locus_tag="PLZ72_01735"
     CDS             complement(34279..34935)
                     /locus_tag="PLZ72_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35045.1"
                     /translation="MEDGTEVRATTTPPASSAPQRGRSTGSAVWTAAMLAAVALTVTA
                     AIASFIMGVDYLPAAALCGNEPTPGCVDGGAGWAATARSIAVALAVVMALLTAAVGVV
                     LTVRTRRGRVVAATVGALLSASAGLISVVTGSRLQWDQLALRSVRVVGPGDYRGMVWM
                     FQSRADDVRFVMVDGVELTVDALRRTAMLSAGSAVGAFVLLIGALLLLRPTPGGTPSG
                     "
     gene            34954..36456
                     /locus_tag="PLZ72_01740"
     CDS             34954..36456
                     /locus_tag="PLZ72_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNA35046.1"
                     /translation="MTKRYLVRTFGCQMNEHDSERLAGLLEADGYEPATDVEDADVVV
                     LNTCCIRENADNKLYGTLGHLKSVKDRRPDMEIMVAGCLAQKDGDLVAQRAPYVDVVL
                     GTHNVHRAVELLHDHQSHGRSVTEILSETVAEDHELFPSALPVVRESGHSAWVTIQIG
                     CDNSCAFCIVPAVRGPEISRPFGDVVAEVQRAVAEGVTQVTLLGQNVNSFGRDLTLRL
                     RSQTGQAPGGVDAATGRPLADPAHLAGARWVAEDTGRARPLFADLLRAVGEVDGLRRV
                     RFTSPHPKDLRPETIAAMAEVPTVCEHLHLPLQSGSDRVLAAMHRGYTAERYLERVDA
                     ARTGIDDLALTTDIIVGFPGETDADFDATLEVAAEARYDNAFTFIYSPRPGTEAAELP
                     HAVPHDVAAERMARLRTVVERTSRLGHEARVGRVEEVMVEGPSRRDPGRLTGRTRHNR
                     LVHFPAEPVLRAGTYARVRVTAASTTNLLGELVEVVAEPTHRRRIPVSVA"
     gene            36453..37154
                     /locus_tag="PLZ72_01745"
     CDS             36453..37154
                     /locus_tag="PLZ72_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35047.1"
                     /translation="MSGTPAGDLPAPRAEPDAPGVLTPDDLPPDPGALVDLLARALAR
                     RHDRLPPDPGRDGPELDPSDALDRARHAVASGWTPHSGSPFERVEPARLLDALDAGIE
                     RVRARSNPPVPTIGRARFSNLALPRSDIAEPLVATAANIRLHRRSAGRPSAPFRLEDR
                     AAVADPHRDLAVAAADVVSTFGPGAVPAFLDAYSRTRRRDQGVEVVDPILLDWWSMIA
                     AIVEGDRDHHVGEEP"
     gene            37151..38167
                     /gene="miaA"
                     /locus_tag="PLZ72_01750"
     CDS             37151..38167
                     /gene="miaA"
                     /locus_tag="PLZ72_01750"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNA35048.1"
                     /translation="MTPAEHPPGRHLVMVGPTASGKSAVAVALARLRTSVGRPTEIVS
                     CDSMAVYRGMDIGTATPTESERGGVPHHLLNVIDPSDEFSVKEFVGLVDDALGDIASR
                     GASAVLVGGTGLYVQAVVDRFDLPGRYPEVAAQLELLGTEELAARLEALDPLAASRVP
                     PGNRRRLVRALEVTIGSGRPFSASGPGMDAYPPTPFVLVGLTVPRDDLGPRIAERYRR
                     QMDAGFLDEVAALDAGAGWSRTAREALGYRELAAHRRGECTLDEAIDTAVTRTRRFAV
                     RQQRWFGRDPRIRWFDAPRPGARDGGDGASVQRCAEDLLGYWSQVAAAGSGPAATVDP
                     SCCD"
     gene            38155..38964
                     /gene="dapF"
                     /locus_tag="PLZ72_01755"
     CDS             38155..38964
                     /gene="dapF"
                     /locus_tag="PLZ72_01755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNA35049.1"
                     /translation="MLRLTKFHGLGNDFLIALAADHPDAEHPLTPDPDVARALCARHT
                     GIGADGLIYGLAPGGDEADVHMVLLNADGSEAEISGNGIRCLAQAVLRHEGRDEGDVL
                     VETAGGVRHLRTVKGSVDGELWIQVDMGEPADGPVPSAEAQEVPARARATVDIGNPHL
                     VLRVDDLDGLDLSEVGPRVERSFDAGINVHAVVVDSAQDLRLRVWERGVGLTQACGSG
                     AVAAAVAAHRWGEVGPHVRVHMPGGEAVVDLDGPRALLTGPSTFVAEVVVP"
     assembly_gap    38970..39069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            39152..40402
                     /gene="hflX"
                     /locus_tag="PLZ72_01760"
     CDS             39152..40402
                     /gene="hflX"
                     /locus_tag="PLZ72_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNA35050.1"
                     /translation="MLVGVALPGVADDALDESLDELELLVDTAGADVVGRVVQRRSAP
                     DPATYVGSGKAEEIRRVAEATDCDTVVFDDELSPAQQRNLEKLLGRTAIDRTAVILDI
                     FGQNAHSQEGKAQVQLAMFRYRLPRLRGRGGSLSQQAGGVGTRRGPGETQLEIDRRRI
                     TRQVHKLEADLRRIAGTRATQRKSRRRSGLPEVVIVGYTNAGKSTLLNRLSGSGVLVE
                     DRLFATLDATTRRLQLPGGESVLLTDTVGFVRKLPHQLVEAFRSTLDVANQADLLVHV
                     VDGAAVDPDGNIDAVRTVLAEIAPDGDPSRIRPELLVFNKADRGPGADRCSARHPGSV
                     VISATTGQGIDDLLLRIGDRLRALLPAVELFVPYERSDVIAALHRAGEVITETPTDDG
                     VMISARIAEDSAPRFAEFVLDRAS"
     gene            40399..>41366
                     /locus_tag="PLZ72_01765"
     CDS             40399..>41366
                     /locus_tag="PLZ72_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA35051.1"
                     /translation="MSTSTAGGFVPPPYPYDRLADIVAVAAAHDGGAVDLSIGTPGDP
                     PAPAVLDALASSGTERGYPPSIGTEALRAAVAEWFDRRFGVGVDPSDVGAAIGTKELV
                     AGLPHWLRLRDPSRDTVLYPEVSYPSYAMGATLAGCRAVPVPMDDRWRLDLSHIDPAD
                     AARALCLWANTPGNPAGGLDDLAAVAEWGRRHRVPVFSDECYVEFTWSGPPARPGQAP
                     GTTVLSSGTEGVVAVHSLSKRSNLAGLRVGFYAGDPELVHYLQEVRKHAGFMVPGPAQ
                     AAAVVAVGDQRDVDVQRERYRTRLARMAGILEGVGIAAPLPDGGFYL"
BASE COUNT         5925 a        14919 c        14377 g         5945 t
ORIGIN      
        1 gtgccgacga gagcgaacgc tcccaccgcc aggaccgaga tgcggactct acgtctgatg
       61 cgtcggctgc tggtccggta cgccgacacc cagaccatgc ccagagcgac cgaggcgagc
      121 gccgctgtcg cgaaggtcgg tgtcggccat cccaggtgca gagcagccag agcgatcagc
      181 gctgcggacg cggcgccggc gatgcggctc cggcggtcct cccacgccag ggcgaagcag
      241 agacccagtg cgatcagccc ggccacgctc cagcgtccgg cggcgccgac gacgacgagg
      301 ccggcggcgg cggccaggct ccaacggcgg gcccgagccc cggcgatcgt gctcaccacg
      361 accagcgcga cccgccacac gacgtcgaag aggtcggttc cggtcggcgc cgcgtccgac
      421 gtcagcgcgg cgagggaccc tgcgacgacg ccgatggccg agacggcgac gatctgggtt
      481 cgggtggcgg gacggcgcat gcgccgggtc ctctgcgggc gcggcggagc cggggcggta
      541 ccgacggtca ccgcaccagt gtgccccagc cgcccggagc cgccgttggg tacatccccc
      601 ctttgctgat gaactccggg gaccggcgcg ggcgacgctc agatctccgg ggcggtggat
      661 cgtggtacca atgggcatgg cccgtcgtcg tcgaaccgct gctcgtcgcg ccccccgcca
      721 gtacagccgc accgatcgcc tcggcgagct ggtgcgtgag gtcgtggcct ccgagttgga
      781 gcgcatcggc gacgagcgtc tggagctcgt caccatcacc gacgccaccg tcgatggatc
      841 cctcgagcac gccgacgtct tctggtcggc gctgcaggcc gaggacgacg gccgtctcga
      901 cgaggtggtc gaggcgctcg aggagcagcg ctggaaggtg cagcaggtgg tcaaccgcga
      961 ggtccgggcc cgaaagaccc ctcagatcag cttccgtcac gacgacgtcc tgcggtccgc
     1021 tctgcgcgtc gaccagatcc tccgcgacat cgagcccggc gcgaccggtg cccccgaacc
     1081 cgacggcgga tccgacggcg aacccgacgg cgacccagac gacggatcgt gaacgccgac
     1141 ggcaaccccg acgcgccgct cgggttgtgc gtggtcgaca agccgtcggg ctggaccagc
     1201 cacgacgtgg tgggccggtg ccggcggatc ttcggcaccc gcaagatcgg ccattccggc
     1261 accctcgacc cgatggccac cggggtgctc gtcctcggag tcggtcgcgc cacccggctc
     1321 ctcaccttcc tcggcggtct gcccaagacc tacgaggccg tgatccgcat gggggtcgag
     1381 accgactcgc tcgacgccga cggggaggtc accgtcaccc accggatgga tgcccccgac
     1441 ctcgaggcgg tgcgggccgc ggcccgtcag ctgaccggtg atctgcaaca ggtcccgccg
     1501 atggtgtcgg ccaagaaggt cgacggacgt cgtctccacg agctcgcccg gcgcggcgag
     1561 gtcgtcgagc gggccccggt cccggtgacc gtgacccgct tcgacgtcga ggcgaccgat
     1621 gacccgatgg tcgtcggcgc cgtcgtgagc tgctcggccg gaacctatgt ccgggtcctc
     1681 gcctccgacc tcggtcacgc tctgggcggg ggagcccacc tggtcgggct gcgtcggacc
     1741 gccgtcgggc cgttcgatct gtccgacgcg gcgacgctcg agcagctcga ggcggatccg
     1801 tccgcccacc tcctggcgcc caccgccatc gagcgcgtga tgagctgcgt ggtcgccgat
     1861 ccggcggtgg ccggcgaggt cctccacggc cgggtcctcg ggcgtgaccg cctcggcctc
     1921 ggcgacgacg gggacgacac cggtccgtgg gcggtgctcg acccggatcg atcccggctc
     1981 ctggcggtct acgccccgca tcgggagggc accgtcaagc ccgcgttcgt cctgtcctga
     2041 gcggccgatc acggtgcgcc cgggctctcc ggccaccggt agcctccggc ccgtggaggt
     2101 ccaccgactg aaccaggtcc gctccgccgg cgcctcgacg acctgtcccc gtcccgccga
     2161 ggggtcggtg gtcaccatcg gcgcctacga cggtgtccac ctggggcacc gggcggtcat
     2221 cggcgatgtc cggcggcgag cggcagccgg cggccacgcg tcggcggtgg tcaccttcga
     2281 ccggcacccg gcgcaggtga tccggcccga gtcggccccg ctcctgctga ccgacctgga
     2341 ccagaagctc gagctgctgg ccgcgaccgg ggtggaccac accctggtgg tcggcttcga
     2401 tcgggagcgg gccgcggagt ccgccgagga cttcgtgcgt acggtcctgg tcgactgcct
     2461 caacgtccgt caggtcgtcg tcggtgagga tttccacttc gggcaccggc gggcgggaaa
     2521 cgtggcgctg ctcgaagagc tcggtgggcc gctggggttc ggcgtgcacg gacacgagct
     2581 ggtgggcgcc gacggccgag cggcccggga cgagacgcag gtgtcgtcca cggcgatccg
     2641 tcgggcgctg gtcgagggcc ggctggccga cgccaacgcg atgctcgggc ggccgcacga
     2701 gatgcggggg ccggtgaccc gtggcgacgc ccgcgggcgg accctcggct tcccgaccgc
     2761 caacgtggcg atccccgctg agatgctcat gcccgctgac ggcatctacg ccggggagct
     2821 ggagctgtcc gacaccggga cggtcctgcc gtcggcgatc tacgtgggca agcggccgac
     2881 cttctacgac gaccgggcga tgacgctgct cgaggtccac gccctcgact tcgccggtga
     2941 catctacgac cgggccacgt cggtgcgctt cacccatcgg atccgtcccg acgcccgctt
     3001 cgcctcggtc gaggagctca ccacccagct ccgactcgac tgcgacgagg cggcccgact
     3061 cctcgggctc cgctgagacc accggtttcc gagcctggtt cggtagattg gtctcctgcc
     3121 ctgtcggcgt gggggtcaac ctgcatgagc cacatcgagt ccagcgagat cgtcgacccg
     3181 atcgaaggtc cggttcggga cgaacccgtc gccccgggcg cggtcgaggt cccgcggctc
     3241 gcccgccacg agatcaccct ggccgacggg cacctggtcg gtgtcaccgt cgccggcgag
     3301 ggcatccctc tggtggtggt ccacgggttc tccgccgagg gcttcctgta cgcccagtcg
     3361 ctttctcggc tggtcggcat ggggttccgg gtgatcgcca tcgacaccgc cggtcacggg
     3421 gcgacgcagg gcctcccgct gtcgggtcag gcgatgaacg actacgccga gctgctcggc
     3481 cgctgcatcg acgagttggg gatcgagcgc ttcgtgctgg ccggtcactc catggggggt
     3541 cagctggtga cccggctcgc ggcgcagtgg ccggaacgga ccatggccgt gatcctcatc
     3601 gacgcgatcg tcggtgacac gtgggaccgc atggtctacc tgttccgggt ggctccgccg
     3661 ctgctgggga tggtcggggc gctcctcgtc gtcgactccc tcaccatccc gccggcgttc
     3721 agcgatcccc gacaggcgac caagctcatg cggctggccg tccccaccgt ggccggtcac
     3781 ctggtgcagc cgtggcggct catggggccg atgatgtcga tcctgcgcac gcggtccagc
     3841 cgctacgcgc tcgacgccat ccgtgagggt gagatcccgg tgatcgccct gcacggcagc
     3901 cgggacatcg cggtgccgca ccgcacgtcc gtcgatgccg tccgtcgcac cgagggcacc
     3961 ctcgtgacca tccagcgcgg cggtcactcc tggccgctca ccgatcccga gaccctcccg
     4021 gcgatcatgg ccgagatgct cgacggaccg ctcggcgacg gcatccgccg ctccctccgc
     4081 gccgccgggg tgcggacccg gaccccgagc atcgaccaga tccgctccgc ctgctacgac
     4141 cccgacgccc gggtgttcga cctcgacccg gtcgatgccc ggcgcaaggt ggtcggacgg
     4201 catcgcaagc cgcgctaccg ctggacggtg gagcatcccc gaccgacggg ctcggcaccg
     4261 ccgaccccga acggtcgcga gcgcacggac cggcggtccc cgacgaccgc cggcggccgc
     4321 aaggcccgcc cggcgaagct cgacgtggtg gacggcgacg cctgactggt agcgtgtgcg
     4381 gtccccggca cccgccggga ccgctcccgt tcccgcggcc ccacctcccg gtggtgtcga
     4441 cggtccgtgg gagccgggtc cgacacccac aggagagcaa ccacgatgac caaccgggag
     4501 cccaccaacc tcccgcccaa gggcgacacg atcgcgaagt tccgcaagca cgacaccgac
     4561 acgggttcgc ccgaggtgca gatcgctctg ctgacggacc gcatcaacca cctgaccgag
     4621 cacctcaagg tgcacaagaa ggaccaccac tcgcggcgcg gtctcctgat gctggtcggc
     4681 aagcgccgac ggttcctcga ctacctcaag aacaacgacg tcgagcgcta ccggaccgtc
     4741 atcgccgagc tcgggctccg tcgctgaacc gttccccacg cggcctacgg gccacgactc
     4801 cgatcgccgt cgaacgccct cccgggactc ctcgggaggg cgttcacgtt ccggaggtcc
     4861 gcatcgggta gcgtggtgtc aacacacatg cggcaggccg gtcagttacc ggttgccgat
     4921 ctccggccct ccggccgatc ccctcggacg aagaggccct tgcggccctc ccgggtgaca
     4981 cccgcagacc cgttccgctc ggtggtcacc accccggggg tcagcagggg cccagccggg
     5041 gctcgaccac tgggaactgg ccccgccgca ccgaacgctc ccgccgtgcg ggggtagaag
     5101 gagggccaca tggccgaacc cacacgcgtc gccgcgccga tcggcggcga cgacaagacc
     5161 atctcgttcg agacgggcaa gctggcggga ctcgccggcg gtgccgtcat ggccgggatg
     5221 ggcaacaccc gcatcctggt gaccgcgacc ggcgccaagc acgtccgcga cgggatcgac
     5281 ttcttcccgc tgaccgtcga catcgaagag cgcatgtacg ccgcgggcaa gatccccggt
     5341 tcgttcttcc ggcgtgaggg tcgggcgccc gagtccgcca tcctcacctg ccgcctgatc
     5401 gaccgtccgc tgcgtccgtc cttccccgac ggcttccgca acgaggtcca catcgtcggc
     5461 accgtcatgg gcgccgacca gcagaacccc tacgacgtgc tggccatcaa cgcggcgtcc
     5521 gccgcgctga tgatctccga cgtgcccttc gaaggcccga tcggcgccgt ccgtctggcc
     5581 tacagcaccg acggcgagtg gatcgcgcac ccgacctacg aggagtccga cgaggcgacc
     5641 ttcgagatcg tcgtcgccgg ccggcagctc gacgacggcg acgtcgccgt gatgatggtc
     5701 gaagccggtg gcaccgagaa gtcgttccag tactacgacg agggtgcacc caaggtcgac
     5761 gaggccgcgc tggccaccgc cctgcaggag tgcaagcagt acatcaaggc cgtcatcgcc
     5821 ctccagcagg agctggtcga cgccgtcggc gccaagccga ccatgtcatt cgacgtccag
     5881 gtcgactaca ccgacgacat ctacgcccag gtcaagagcc tgggcggcga acgcatgaac
     5941 gagatcatgc agatcgccga caagtccgag cgcggcgccg ccgaggacgc cctgcgcacc
     6001 gagatggtcg accggatcgt ccccgagctc gtcaccgcgg ccgtcgccga gggagcggac
     6061 gaggccacgg cgcaggcgca ggccaccaag caggtcaaga gcgccgtccg gtccctgtcc
     6121 aagaacacgg tgcgcacccg gatcgtcaac gacggcatgc gcatcgacgg ccgagggctg
     6181 cgcgacatcc gaccgctcag ctccgaggtc ggtgtgctgc ccaccgctca cggctccgga
     6241 ctgttccagc gtggcgagac ccaggtgctc aacatctgca cgctcggcat ggccaagatg
     6301 gaccagatga tcgacggcat cgacccgatc acccgcaagc gctacctgca ccactacaac
     6361 ttcccgccgt tctccaccgg cgagaccggc ttcatgcggg gcccgaagcg ccgcgagatc
     6421 ggccacggcg ctctcgccga gcgcgccgtg ttcccggtcg tgccgtcgct cgaggagttc
     6481 ccctacacca tccgtctggt ctccgaggtc ctctcctcca acggatcgac gtcgatgggc
     6541 tcggtctgct cgtcgaccct gtccctgatg gacgccggcg tgccgatcaa ggcaccggtg
     6601 gcgggcatcg ccatgggcct cgtccacgcc gatggccagt acgcgaccct gaccgacatc
     6661 ctcggggccg aggacgcctt cggcgacatg gacttcaagg tggcgggcac ggcggacgcg
     6721 gtgacggcgc tgcagctcga cacgaagatc gacggcatcc ccgccgacgt gctggctgcc
     6781 gctctcgagc aggccaagga ggcccgcctc cagatcctcg acgtcatggc cggcgccatc
     6841 gccgagcccc ggcccgaggt caacgagacc gcaccgaaga tcatcagctt cgagatcccg
     6901 gccgacaaga tcggggaggt catcgggccc aagggcaagg tcatcaactc gatccagagc
     6961 gagaccggcg ccaacatcag cgtcgacgac gacggcctgg tcggcatcgt gtcgatcgcg
     7021 gcggtcgacg gcggtgccgt ggccgaggcc gagcggcaga tcaagctgat cctcgatccg
     7081 ccgaccgctg agctcggggc cacctacgac ggccgcgtcg tgaacatcac caagttcggg
     7141 gcgttcgtca acatcctccc gggacgtgac gggctggtgc acatctccaa gctcggtggc
     7201 ggcaagcgga tcgacaaggt cgaggacgtc ctcaccctgg gcgacgcgat caccgtgaag
     7261 gtcgacgaca tcgaccccaa cgggaagatc tcgctgtcgc cggtcggcga gctcgcgggc
     7321 ggtgacaacc caaaagcgag tgacggcgtc ggctccgccg acgccgtgag cgccgcgggt
     7381 ccggactccg aggagcgctc cgccgatcgg cgtgagctcg acttcggcgc cgcgttcgac
     7441 gccgagctcc gcgaggagtt cggcgacctc ggtcccgccc ccgtccgctc cggtggcgga
     7501 ggcgaccgcg gtcgcggggg tcgcggtcgc ggcggcaacc gccgccggtg accgaccggt
     7561 cagtgccggt gccgtcgggc ggggggagcg tgctcccctc gcccgacgag caggtccgtc
     7621 gaaccgtgct cgacaacggg ctccgggtcg tcaccgatcg actgcccacg tcgcgctcgg
     7681 tggctgcttc ggtctgggtc ggcgtcggcg gccgcgacga gtccgatgag atgtcgggcg
     7741 cgtcgcactt ccttgagcac ctcctgttca agggcaccga cgagcgtgac gccaagtcga
     7801 tcgcggtggc gatcgacggg atcggcggcg acatgaacgc gttcaccgcc aacgagcaca
     7861 ccgcgttcta cgcacggatg ccggcggtcg aggcccatgt cggcatcgac ctgctgctcg
     7921 acgtcgtcca gaacccggcg ctgagggatt gggagttcga cggcgaacgc gaggtgatcc
     7981 tcgaggagct cgcggccgcg gaggacgatc cggacgacgt ctcctcggtg aagctgttcg
     8041 aacggctctt cccggaccat ccgctgggcc gtgaggtgct gggctcgatc gaatcgatcg
     8101 acgggctgac ccgcgacgcc gtccgcgact tcttcgggga gtggtacacg ccggccaacc
     8161 tggtcgtggc tgctgccggc gacatcgacc acgacctgct ggtcgacgag gtcgaccggc
     8221 ggttcggcga ccgttccccc ggaccggtgc cgacgcgcac cgagcccgac gaccgcctcg
     8281 gcgagtcgag cgtcgaggtc cggccgatcg aggtcgtgca cctggcgatg ggctggcggg
     8341 cgctcgccgt cgacgacgac gggcgctatg ccctggcgct gctcaaccac gtgttcggct
     8401 ccgggccgtc gagtcggctg ttccaggagg tccgcgagga gcgcggcctc acctactcga
     8461 tctcgtcggc ggtgagccac cacgtcgaca ccggtgcggt gtcggtccac tgcgcgacgg
     8521 ccccgggcaa ggtcgacgac ctgatcgccg tcgtcgacac gatcctcgac gacgtggtgt
     8581 cgcacggcat cagccgcgag gagctggccc gggccaaggg ctcgctccgg ggcgcgtacc
     8641 tgatgtccta cgaggacgtc ggcacgcgca tgaccaggct gggcatgagc gagaccatgc
     8701 ggggcgggct caccccgatc gacgagcacc tcgcccggct ggaagccgtg accgacgacg
     8761 aggtgcagcg ggtggccgag caggtcctcg gctcagcccg ggtgatgtcg agcgtcggtc
     8821 ctcgctgaac gacgctcgac cgttcgggat ggcgggtcga ccgcaccgcg gcgatcaacc
     8881 cgtgtcggtg tcggcccgct cgacctcgtc gcgggtcacg ccgaggacga acaggatcgc
     8941 gtcgaggtac gggacgttga cggtggtgtc cgccgcctcc tggaccacgg gcttggcgtt
     9001 gaaggcgacc cccaaaccgg cggtcgccag catgtccagg tcgttggcgc cgtcgccgac
     9061 cgcgaccgtc tggctcagcg ggatcccctc cgcctcggcg aaggaccgca ggaaccgggc
     9121 cttcgctccc cggtcgacga cctcgccgac gacccgcccg gtcagtcgtc cgtcgtgcac
     9181 ctccagctgg ttcgcccggg cgtggtccac gccgagctcg gcggccagac cgtcggtgaa
     9241 gtgggtgaac ccgcccgaca cgatgcccac ggtgtagccc agccgcttga gggtgcgcac
     9301 gaacgtgcgg gccccggggg tgaggcgcat ccgggcccgg gcccggtcca gggcgtcgac
     9361 ctcgaggccc ttcaggcggg ccacccgctc ggtcagggcc gcggtgaagt ccagctcgcc
     9421 ggacatcgcc cgctcggtca cggctcgtac ttcgtccagg cacccggcct cggccgcgat
     9481 caactcgatc acctcgtcct ggatcagcgt cgagtcgacg tccagcacga ccaggcgctt
     9541 ggcccgacgc tccagcccgg cgggctgacg ggcgatgtcg aggcggtgca gggcggctgc
     9601 ctcgcccagg tgctgggcca gcgcggtcgg ctcggcaccg gtgacccgga actcgtacgc
     9661 gtacaccgga tagcgggcca ggcgcacgat ccggtcgatg ttgccccccg cggcgttgat
     9721 cccaccggcg acggctgcga tctccgccgg cgacagcggg gtctcggttg cgtggccgag
     9781 cacggtgacc gcctcggtgc gctggtgcgc cggtcggggc gggggagcag acgacagctc
     9841 cacgcgaacg ccgcggccct ccgccagtgg gtcgagcagt tggggaagcc gatcccggag
     9901 gtcgccgggg tccgacacgg ccacggccag ggtcagctgt ccgcgcacga gcacctgttc
     9961 gacgtcctgg acctccacct cggccagatc cagcagcgtc atcaggtccg cggtgatccc
    10021 ggggtgatcc gggccgctca cgcgcagcag cagcgcggcg gtgggcgccg gggatggatc
    10081 gtccggtgcg atcggcatgc ccggtacggt acggcggttc cgcgacgggg gtgacaatgg
    10141 gtctggtgga tgtcgaatcg cacggcgagc acgtgcgcat cgtccggttg gaccgccccg
    10201 agcggctcaa cgcgctgtcg atcgacctgg ccgttgagct cgactcggcg ctggcgtcgg
    10261 tgggagccga caactcggcc cgcgtcgtga tcctgaccgg cacagggcgg gcgttctgct
    10321 ctggcctcga cctcaaggac tacggcgtca tccccaacat cgacggcctg acggtccacc
    10381 gcatcgccag ccgttccatg cggacctact cgcagatcac ccggcggctg cgctcgatcc
    10441 cccagcccgt catcgccgcg gtcaacggcc cggcctacgg cggcgggatg tgcctggctc
    10501 tcggggcgga cctgcggatc gcggacgaga cggcgtcgtt caacgcgacc ggcatcgtca
    10561 acggcctcac ctcggccgag atgggagccg gatggctgct gccgcgactg atcggagctg
    10621 ccaacgccaa cgacatcctg ttgaccggac gccgcgtcga cgcggccgag gcccagcgga
    10681 tgggactggt gtcgagggtg gtcccggccg ggaccgtgct cgacgaggct ctggagatgg
    10741 cggcggacat ggtgcgctac tcgcaccacg gcctcgaggc gaccaagcag gcgctgtggg
    10801 tcgagctcga gatcaacagc ctcgacgccg ccatcgagtt cgaggaccgc aaccagctca
    10861 tggccggctt caccgacaac ctgcccacgg ccatccgggc cttcgaccgc gacgagcagc
    10921 cggtgtacct cgacgagccc cggcgcgacc tgttcgagca acccgggggc tgaccgtcgc
    10981 cgcagtcaca cggtcggaac ccgaccctgg cgcggaccga tcccagcgaa cgaccctggc
    11041 gcggaacgac cctgtggcgc caccgctact gtcggagcgt gatcgggatg cagcggtgat
    11101 cagggtcggg gtcctcggag cgggcggccg catgggcgcg acggtgtgcg aggcggtcca
    11161 cggcgacgac gacctcagcc tggtcgcggc ggtcgacccc agctactcgg gtctggacct
    11221 ccggaccgtg accggggtcg acgccggtat ccgcatcgcc agcgggccgg cggcgctcga
    11281 gcgcggtgcc gtccaggtgg tggtcgactt cagccaccgg gccgcggcga tggacaacct
    11341 gaggtggtgc gcggagcacg gcgtccacgc ggtggtcggc accaccgggt tcaccgacga
    11401 cgacatggcg gccttcgacg cgatgttcac acgctccaac tgcgtcatcg cgcccaactt
    11461 cgcgatcggt gcggtcctga tgatgcactt cgccgaggtg gcggccccgt acttcgactc
    11521 cgccgagatc atcgagctgc accacgagaa caagctcgat gccccgtcgg gcaccgcggt
    11581 gcacaccgcc cagcggatgg ccgaggcgtc caaggactgg accccggacc ccaccacctc
    11641 ggagctgatc gagggggccc ggggcggggt cggaccggcc gacatccaca tccactcggt
    11701 ccgcctgcgg ggcctcgtcg cgcaccagga ggtgctgctg ggcaccgccg ggcagaccct
    11761 gacgctgcgt cacgactcga tcgaccgcac cagcttcatg ccgggggtcg tgctcgcggt
    11821 caagaaggtc gcggaccgac ccggggtgac cgtcggcctc gaagcgctgc tcgacctgcc
    11881 gggctgatcg ccggctgcag caccggcaag gcgctcagtc gtcgccgagc tcgtcctcga
    11941 gctcctcgag cccaccgacc aaccctcggt ccgacagcat ctgccacacg accagcgcgg
    12001 cgaccagagc gaccgacccc cacagcaacc aggtctggta gcggtcgacc acgtcgagca
    12061 cgtgttcgat ctggtcctcg aagaggtggc ccagccagcg catgaggagc acccggccca
    12121 cggtgccgac caggttcagg gcgatgaacc ggcgcagcgg gaagcggacg acgccggcga
    12181 ccaggcacac gaggttgttg ggggcgacca cgacgaggac gttgaggacc cggtggacgg
    12241 ctcgggggtc ctcgtggacc cggtcgagca gatgcgtccc gcccgggaag gtccgtcgcg
    12301 cccagtgcag ggcccggtcg ccgtagagcc agcccatggc gaagaagatc gggtcagggg
    12361 cgagcaaccg aaggctcgcc accaccacca tcggggccag gtccaggctg atcgaggtca
    12421 tcagcaggta cttgttcgac gagttgagcg cgatcaggcc cagcggccgg gactgcaccc
    12481 agtcggccca gaccatgttg cccaggttcg aggcgatcac cagcaggacg atcccggcgg
    12541 tcagcagcgg gagcagccac cgagggtggg tctggtggtc gggcggcaga gccggggcgg
    12601 cgtaggacat gggctcaggg cgctcgggag ggttgggccg tcgcggactc cgcgctcggc
    12661 ctacgctcga ccctacaggc ggtggctccc ggttgggtgg cgccgctgga aggaccatcg
    12721 accatccgcc gggaacgggg aacgcctcgc cggccatccg acagggggaa ccgtgcaggg
    12781 cacgatgcag gaccgtccgc tgacgctcaa ccacctcttc gagcgcgccg agcggctcaa
    12841 cccgaccaag gaggtcgtca ccgcgacggc caccggcgtg gaccgctaca cctacggcga
    12901 gtgggccgac cgcacccgcc ggctcgccgc agccctcgac tcgctcggga tctcgcccga
    12961 cggccgcgtc gcgacgttcg cctggaacac cggacgccac ctcgagctct acttcgccgc
    13021 cccgtgctcc ggtcgggtgc tgcacacgct caacatccgg ctgttccccg aacaggtcac
    13081 ctacatcgtc aaccacgccg aggacgaggt catcttcgtc gaccgctcgc tgctcggcct
    13141 cctcgccccg ttgatgccga cgttccgcac cgtccgccac gtcgtggtca tggaggacgg
    13201 gggccagggt ccggaccccg agggcgtcga ggtcctcgac tacgaagagc tgatcggcgc
    13261 ccaggcgccc gccgagttcg gcgtcgacga cgagtaccgg gccgcgtcga tgtgctacac
    13321 gtcgggcacc accggcaacc ccaagggcgt cgtctacacg caccgcagca cgttcctgca
    13381 cacgatgggg gcgatgctgg ccgactcggt cggggtctgc gagagcgacg tcgtcctgcc
    13441 ggtcgtgccg atgttccacg ccaacgcctg ggggctcgcc cacgccgggg tcgcgtcggg
    13501 ggccaagctc gtcatgcccg gcccgaacat ggtcccggtc aagctggccg agatgatcga
    13561 gtccgaggac gtcaccctgg ccgcgggagt ccccaccatc tggatgggcg tcctgcccga
    13621 gctggccgga cgcgacacgt cgtcgctgcg ggccatcccg tgcggcggct ccgcggttcc
    13681 caaggctctg tcggaggcct accgggccca gatcggcctg ccgatcctcc aggcatgggg
    13741 gatgaccgag acctccccgg tggcgtcggt ggccaacgtc accaccgtcc tcgaagggct
    13801 ccccgaggac gagctggccg acgtccgggc cagccagggt cgtccggtgc tcggcgtcga
    13861 gctgcgcatc gcggaccagg cgacggggga gatcctgccg tgggacggcc agagccgtgg
    13921 tgagctgcag gctgccggtc cgtggatcgc cgcgggctac tacgacgacc cccgatcgcc
    13981 ggagtcgttc acagatgacg gctggctcaa gaccggcgac gtggcggtga tgacccccga
    14041 gggctacgtc cgtatcgtcg accgcaccaa ggacgtcgtg aagtccggcg gcgagtggat
    14101 cagctccgtg gagctcgaga acgagatcat ggcgcacccc aaggtcgccg aggcggcggt
    14161 gatcggcgtc ccgcacccga agtgggccga gcggccgctg gcctgcgtgg tggtcaagcc
    14221 cggggaggag ctgaccaagg aggaggtcgt cgagttcctc gacggccggg tcgccaagtg
    14281 gtggctgccc gacgacgtcg tcttcatcga cgaggtcccc aagaccagcg tcgggaagtt
    14341 ctccaagaag gacctgcggg accgcttcga cgactacacg cttcccaccg ccggcgactg
    14401 atcgcccgtc ggtcggtccc cacgggtgca gatccgggcc gacgcccggt tctgcacccg
    14461 ttcaccgcgg gtcgtagggt cggtcgacga tgtacgactt cctccgccgc ccggcctgga
    14521 tcgtcagcca cgtcctggtc gtgatcgggg tcctggccat gatcggcctc ggcttctggc
    14581 agcgtcagcg ctggatcgac gagagggtga agtccgatgc gttggccgcc caggccgcgg
    14641 gtacccccgt cccgctcgac gaggcgctcg ccgactccgg cgtcgatgcg tcgacggcac
    14701 ccgccgcggt tcccgagtcg gtggagttcc gtcgggtcga ggtgagcggc gtctacgacg
    14761 cggccggtga ggtgctggtg cgcaaccgca gccagggcgg tgcgcccggg gcgtgggtgc
    14821 tcaccccgct ggtgcaggcc gacggcaccg cggtcgccgt cgtccgcggt tgggtgccgc
    14881 tcgacatcga ccctccggcc ccgccgttcc caggctccga gccaccggcg ggagaggtga
    14941 cggtgaccgg gctggtgcag cgcagtcagc agcacgggac cttcggcggg accgatccgg
    15001 cgaccgggac cctccggacc ctcgcccggg tcgacgtccc gcggctggac cagcaggtcg
    15061 acgccgcgtt cgaaccggtc tacgtgatgc tcgacggcca gacgcccccg cagccggcat
    15121 cgaccgacgg catgcccgag ccggtcaagc tcgagctgcc gacacccgag accaacttct
    15181 cctacatggt ccagtggtgg atcttcgccc tgatcgccgc cgccggctac ccgctggtcc
    15241 tgcggcgggt cgcgcagaac cgcgtccggg gcgaccagac ccccgacgac gatcccgtcg
    15301 atctcccggg gagcggggca gcggacgtcc cggtggcgac ggggaacgac gaggttccga
    15361 cctgatggcc gaggacggcg cggccggacc ggtggacgtc cgggagcgga tcatgttcgc
    15421 ggcggtccga tgcgtggagc gtggcggggt gcgcgacgtg tcgatggagg ccgtcgcggc
    15481 cgaggccggc ctgtcgcgca ccacgatcta ccggcacttc cccggcgggc ggagccagct
    15541 ggtgtccgag accgccacct gggaggtggc gcgcttctgg cgacggctgg cgctggcggt
    15601 ggccgacctc gagacgctcg aggaccggct ggtgaccggc ttggtgatcg ggaccaagct
    15661 gatccgccgc agcagcatca tggccaacct gctcgaaccg gagatcgacg agctcgtgaa
    15721 cgctctccag cccagcgagt cgatcgtcca ccaggtnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnccgg cccgtccgcg ccgggcgtgg cgttgggcac gtacctcgag
    15901 cccgacagcg ccgaagcgcg cgtggtcgac gccctgttcg actgcgtcgc ccgttggggc
    15961 atggccaaga ccaccgtcga agacgtcgcc cgcagcgccg ggatctcccg cgccaccgtc
    16021 taccggctgt tcccgggggg caagggggcc atcctccagg ccggtctcgg cagcgagatc
    16081 acccgcctgg tcggtgagct gaaccgccag ctggaacccc tcgccaccct cgaggaagtg
    16141 ctggcccggg cggtcagctt ctcgatcacc gagatgcgcg agcaccgggc gttcggacag
    16201 gtccccgaac acgagtgggc cagcatcgag aagcacctga ccttcgatcg cctcgacccg
    16261 gtcttcctgc tcgccggcgc cgccttcggt ccgtcgctca tgcgcttcct cacaccagag
    16321 gacgcgagcc acgtcgccat gtgggcggcg cgactggtcg tgtcgtacct gcagagcccc
    16381 tccgacgacc tcgaccccgg ccgggaggac accgcccggc acctggtcgt gacctacctg
    16441 ctgcccggcc tcgagccggc ggcctgacct ccatccgtcc acaaccactc gatacccaca
    16501 caggagcaca tgtcatgagc atcaacgagg agatcctcgg ccgagccgac gtcgacgacc
    16561 tggaggccat cctcgcggtg tccaacagcg atcgcgacgc cgtcatctcc acggtccagc
    16621 agaacgccga ggccatcttc acctgggact acaccaaggg tgcccggccg gctctggaca
    16681 agctctacga gaaggccaag gtctccatgt ggaacggcga gaccgacctc ccctgggaca
    16741 ccgaggtgga ccaggagtcg atcgtcatcg ccaacgccga gcagaactcg ggcgtgaacg
    16801 gcgccggcgc gctgggcctg gccgacttcg acgtgacggg caccccgttc gagaagtgga
    16861 ctcccgacca gtggatgcgg ctgggcatcg agagccagaa ctggaccctc agccagttca
    16921 tgcacggcga gcagggcgct ctcatctgca ccgccaagat cgtcgagacc gtgccgtgga
    16981 tcgacgccaa gtactacgcc tccacccagg tcatggacga ggcccgtcac gtcgaggtgt
    17041 tcgcccgcta cctcgacacc aagctgtccg gtcactaccc ggtcaacgcg cacctcaaga
    17101 tgctgctcga cgacatcatc accgacagcc gctgggacat gacctacctc ggcatgcagg
    17161 tgatggtcga ggggctggcc ctggcctcct tcggcttcat gcaccagatg tcgaccgagc
    17221 cgctgctcaa gcagctgctc cgctacgtga tgagcgacga ggcccgccac gtcgccttcg
    17281 gcgtgctgtc gctccaggag tactacgcca acctgtccga cgccgagatc ttcgagcgcc
    17341 agcagttcgc attcgaggcc gcggtgcgca tgcgggacag gttcctccag caggaggtct
    17401 gggagcgcat ggacgtcccg gtcaaggacg ccatcgaggc tgtgatgcgg acgccgaccc
    17461 gccagctgtt ccagataatg ctgttctcca agatcgtccc gaactgcaag aagctgggcc
    17521 tgctcgaccg caacgacgcc tggctgcgcc gccggttcga ggagatcggc gtcatccagt
    17581 tcgaggactg ggaggacacc ggctcggagt acgagtcgtt cgccctcacc gacgcggacc
    17641 agcgcagcgc ctgatacgcg gcgccaccca cgagctcgac gacggcgggt cccggcgggg
    17701 cccgccgtcg gcgcgctcgg atgggagatg aggggcgcgt cggtgaggtt cgggccggcg
    17761 aacccggtcg ggtcggtcat gggccctcca gtagcctgag ctcgatgtcc gacgacctgg
    17821 ccacggtgct gctccagctg cgcctgcggg cattcgccac caccgatgtt ctgagggcgg
    17881 cgacggccgt ggacggtgac cggctcgacg aggtcctgcg aaccgcggag tcggatcggc
    17941 tggtgcgtca ccgcgagggg cggatcgtgg gatggtcgct caccgcggcg ggacggaccc
    18001 gaggccagga gctcctgtcg gcggagttgg acgccgcggg cacacgcgac gccgtcctcg
    18061 acgcgtacgg cgccttcctg ccgctcaacg ccgagctcct gtccatctgc accgactggc
    18121 aggtcgtcgt cgtcgacggc gagcacgtcc ccaacgacca ctccgatccg gagcgtgatt
    18181 cgtcggtgct ggcgcgactc gcacggctgc acccggccgc catcgaggtg acgtccgctc
    18241 tcggtcggac ggtgccgcgc ttcacgggct acggtccgag gctcatcgag tcccacgacc
    18301 acgtcctcgc cggtcgcacc gagtggctga cccgggtgac cggcgactcc tatcacggcg
    18361 tgtggttcga gttgcacgag cacctgctgg ccgtgctcgg ccgggaccgc gagcacgaag
    18421 ccaccccgga cgccatcccc acgaacgcag ccggatccgg ccgccccggc cgccgggccc
    18481 caggaactgg agacacccca tgagcgcgac cttcggacgc gtcatcaccg ccatggtgac
    18541 ccccttcgac gaccgcggcg agctcgacct cgacgccgcg gtcgagctgg ccaactggct
    18601 ggtcgagaac ggctccgacg gcctcgtgct gtgcgggacc accggcgaga gcccgacgct
    18661 caccgagtcc gaggagctcg tcttgttccg gacggtccgc ggtgcggtgt cggtcccggt
    18721 gatcgccggg gccggatcga actccaccgc caccgcggtc gagcagaccc gtcgggcatc
    18781 cgagctcggc gtggacgcca tcctgtcggt gacgccgtac tacaaccggc cctcgcaggc
    18841 cggcctcgac gcccacttcc gggccgtcgc cgcggccacc gacctgccgg tcatcctcta
    18901 cgacatcccc gtgcgcaccg gccgcaagat cgacaccgac acgatgctcg gcctggccca
    18961 cgacgtaccc aacatcgtcg gggtcaagga cgcgtcgggt gacccgaccg agacggcccg
    19021 actgatcgcg tcggcgccgt cggccttcga ggtctacagc ggtgacgacg cccagaccct
    19081 gcccctcctg gcggtcggcg ccgtcggcgt catctcggtg gcgtcgcact gggtcggacg
    19141 ccagatgggg gagatgatcg atgccttcga gaagggcgac gtgagcaccg cccgccggat
    19201 caacgccggc ctcctcgact cctacgacta cgagagcagc cccgacgccg tgaaccccgt
    19261 ccccaccaag accatgctcc gcgtgctcgg cctgaaggtc ggctcgtgcc gcccgccgat
    19321 gggccccgcg cccgacgacc tcgaggaccg ggcccgccgc gtgctcgacg ggctggcctg
    19381 atggcgccgc cggttcacat cacgttcctg ggcggcctcg gcgagatcgg ccgcaactgc
    19441 gccgctctcg agctcgaggg ccagatcctg ctcatcgact gcggtctgat gttccccgac
    19501 aacgacatgt tgggcgtcga cctggtcctg cccgacttca cgtggctgct cgagcgctcc
    19561 gacgacatcg tcggctgcat cgccacccac ggtcacgagg accacgtcgg cgggttgtcc
    19621 tacctgctgc gcgaggcgag cttcccgatc ttcggcaccg agctcacgtt gggcctggcc
    19681 cgcgggcgca tcgaggagtc gggcctgatg ggtcgcaccg acctgtgcgt ggccgccgac
    19741 ggggacacct gggagatcgg gccgttcgag gtcgagttca tcccgaccac ccactcggtt
    19801 ccgcaggcgg tcgccaccgc gttccacacc ccgcagggca cgatcgtcca cagcggagac
    19861 ttcaagatcg acctcacgcc cgtcgacggc cggctgacca acctgtcgcg gctgggggcc
    19921 ctggccgacg gcgaggggat ccggctgctg ctggcggact cgaccaacgc cgaccaacgc
    19981 gggcactcgc gctcggagac cgagatcgga cgggtcctgc accagctcat gtacgagcag
    20041 cggggccgtc gggtcatcgt ggcctgcttc gccagccaca tccaccggat ccagcagctc
    20101 gccgacgcag cggtcgactc gggtcgtgtg atcgcgccgc tcggacggtc gatgcgtcgc
    20161 aacatctcga tggcgcggga gatggggctg ctgcgcatcc ctgactccgc actgatcgac
    20221 gtcgaggacg tcgaccgcca cgagccggga aaggtgctgg tgctctgcac cgggtcacag
    20281 ggtgagccga tgtcggcgct cacgttgctc gccggccagg agaaccgctg gctgaagctg
    20341 caccccgacg acacggtcat cctctccagc catccgatcc ccggcaacga gcacgcggtc
    20401 accaaggtga tcgacaacct cttccgacag ggcgtcgagg tcatccactc gggggtgtcc
    20461 gacgtgcacg ccaccgggca cgccaaggcc gaggagctca agctctacca gtcgatcctc
    20521 aagccagact ggttcgtgcc cgtgcacggc gagtaccacc acctggtcgc acacgcccgt
    20581 ctggccgaga cgatgggcac cccgcacgac cacatcgtcg tggccgagga cggcgaccgg
    20641 ctggtcctcg acgacgacgg gctccgcgag atcggccggg tgcccgccga gtacgtctac
    20701 gtccacggca ccgtcggcgg cgtcggaacc ggagtcctgg ccgaccgtcg gatcctcgcc
    20761 gacgagggcg tcgtgtccgc gatcgtctgc gtcgaccgga agaagaagcg gatcgtcgcc
    20821 gggccgcaga tcaccacccg ggggtgggtg tacgaggagg agtcggccga cctgctgtcg
    20881 gacctggccg agcgggttcg ggccgcggtc gacagcggat tggccgagga cgcagaggtg
    20941 tcgaggctgg tcaagctcgt gcgcaaggca gcgggcagct tcgtgtcgga ccgcacccgg
    21001 cgtcgaccga tgatcgttcc ggtcgtgctc gaggcctgac gcccgcgtcc accgcaccga
    21061 acgtgtagcc ggcggtcgct ggcgtgacct gtgggctaca agttcggcgg cggctggcga
    21121 tgttgtagcc ggcggtcgct ggcgtgacct gtgggctaca agttcggcgg cgagggcggg
    21181 tgggggtgac ggatgtcacc cacgtccgag gtgctgcctg gcgcgagcgc gcagcagact
    21241 gaccccgatc gagccggaac gtgatgtggc gcgctcgttc ggggccctcc gcgagccccc
    21301 gaccagaagg gacccccacg tgttcggacg actcggagcc tggtgctgcc gacggcggtg
    21361 gatcgtgctc gccgcgtggc tggtggtgct cgtcgccggc ggcgcgctca ccggcgcgct
    21421 cggaagcaac ttcaagtcgg agttcaacct ccccaacgtc gagtccaagc acggcatgga
    21481 cctgctggcc tccgacttcg gcgggatcgg agccggttca tcgggctcga tcgtgttccg
    21541 caccgacggc cagttctccg acgcccagcg ggcagagctc gaggcgttcc tcgacgatgt
    21601 cgcggatgtc gacgacacca ctgtccgcag ccccttcggg ccgaccggcg ccccccagat
    21661 ctcgacccgg ggcgacgacg gaaccatcgc gtacgccgac atccaggtcc cggtcctgct
    21721 cgaccaggag ggcgcatccg agtacgccga ggaggtccgc gggctcgccc cccacgtcga
    21781 cggtgtcgac atcgcgtacg gcggtcagct gttcgtcgag ttcgagcccc ccgactcgga
    21841 gctgctcggc ctggccttcg ccatcgtcat tctcatcacg gcgttcggtt cggtcctggc
    21901 gatggggctg ccgatcggcg tcgccctagg ggggatcggc gtgggctcga tcctgctcac
    21961 gctcctgtcg aacacggtcc ccatgcccga cttcgccacc acgctgggcg tgatgatcgg
    22021 cctgggtgtg ggcatcgact acgcgctctt catcgtcacc cgcttccgcg agcaactcca
    22081 cctgggccac acggtcgagg agtccgtcgc catctcactc gacacgtccg gccgggccgt
    22141 caccttcgcc ggactgaccg tggtgatctc gctgctcggc atgctgttga tgggggtgag
    22201 cttcatccag ggtctcggca tcggcgccgc caccgtcgtc gcggtgacca tggtggcgtc
    22261 gctcaccctg ctgcccgcca tgctcggctt cgccggcgag cgcgtcgaac ggacccgctg
    22321 gcggggtctg atcgccgccg gactggtcgc ggtggccctg ctcggtgccg ggctcaagat
    22381 ccagcccctc gccgcggggg cgatcctggt cgcggcggtg gtgatcgtgc tcgggttctt
    22441 ctggacgccg ctgcgacgcg aggttccccg ccgcgaaccc gctccgctgc gcaccacccg
    22501 cgcctaccgc tggagtcggg tcgtgcagca ccacccctgg tcgatggcga tcggggcgac
    22561 ggtgttcctg gtggtgctca ccatcccggc gttcgggatg cacctcgggt tccccgacga
    22621 gggcaacgac ccgcccggga ccaccacccg ggaggcctac gacctgctgg ccgagggctt
    22681 cggaccgggc cacaacgggc cgctggtgct cgtgtcgaag ctgccggagg ggacgacccc
    22741 cgagcagctg gcggcggtca ccacggcgct ggaggacacc cccggcgtcg agtcggtgag
    22801 cccggcgctg cccaacacgc tgatcaccgg caaggacggc gaccccaccg cggtcatgtg
    22861 gcgggtcgtc cccaccaccg caccccagga ccaggcgacc accgaactgg tgcacaccct
    22921 gcgggacgag gtcctgcccc gcgcccagac cggcacgggc ctcgacgtcc tcgtcagcgg
    22981 cttcgtcgcg gtgaccgtcg acttcacgtc gttcgtgtcc gaacggctcg tgctgttctt
    23041 cgccgcggtc ctgtcgctgt cgttcctgct gctgatggtg gtgttccggt cgctcctggt
    23101 cccggtcaag gccgtgatca tgaacctgct gtcgatcggc gccgcctacg gcgtgctcgt
    23161 cgccatcttc cagtgggggt gggccaagga catcttcggt gtggagccgg ccccgatcgc
    23221 gccgttcatg ccgatgatgc tgttcgccat cgtgttcggc ctgtcgatgg actacgaggt
    23281 gttcctcctg tcgaggatcc gcgaggagtg ggtccgcacc ggcgacgcca aggcgtcagt
    23341 cgccgacggc cttgccgcca ccgcccgggt catcaccgcg gccgccgcga tcatggtgtt
    23401 cgtgttcggc agcttcctgg gcgagtcgat gcggaccgtc aagctcttcg gcttcggtct
    23461 ggccctggcc gtcctgctcg acgccacggt ggtgcgcatg ttgctggtgc ccgccaccat
    23521 ggagctcctc ggcgacagga actggtggct gccgcggtgg ctggaccgga tcctgccgtc
    23581 ggtcaacatc gaaggcaccc tcgagcacac caaccccggc gaggacgacg acgccgagga
    23641 tgatccgacc gtgccgagct cggatcgtca gcccgaaccg gtctgaccgg cgtcgccgcc
    23701 ccacgccgcc cattacgggg ttctcggggg gttccgcccc actgtcggtc acggtgggta
    23761 gcgtggctga cactgtggcg agcaagcaga cgacctcggc gcggcgttcc agcggtgcca
    23821 accgctccgg atcgcgcgga cgatcgaccc ccaagcggac cgccgcgaag cggtccacgc
    23881 cgaatcgggg gtccacccgc cgggcccagc cgcctcgacc gggcgtcgga gtcctgttcg
    23941 acggtcacgg ccacgacgtg gcgggactgc tgctgatcct ggtcgggatc gtcaccgcgc
    24001 tcggtgtcta cgccggcgcc gccgggccgc tgggttcggc cctggcgtcg ctgtccgccg
    24061 gcctggtcgg acgtctggac ctggtcgtgc cgcccctgct ggtggcgggc ggcgtcctgg
    24121 tggtgatcgg tccgcgtcgg gccgacgtcg acctgacggg ggagtccggt gacaccgcct
    24181 ccggtcgcgg cgccgtcgac ccccacgagc gtcacgtggc ccggatccgc cgcggcatcg
    24241 gcatcggtct cgccatcctc accctgctcg ggttcctcga cctggcctcc ggggccgggc
    24301 tcaccgtcga ggccgacggg ctcgacgcct tcgccgcggc cggcgggctg ctcggagctg
    24361 cggtcgccgg cgtgttcgag gccgtcgtcg ggagcggggg agctgtcgcg gtcctggtgg
    24421 tcctcgccgt tctgtcggcc agcctgatga ccggccggcc gctgcggacc gtggcctccg
    24481 atgtcggaca cgccgccgcg ccgctgctcc gacggatcgg ggtgtggttc tcggacctgt
    24541 tccgggtcgg ctccgccgag gcggccgacg gggacactgc ggatcccgcc ccggacgtcg
    24601 ccctgttcga ccaggatcgg gacgccaagc ccgcacccaa gcggagccgg ccccgcaagc
    24661 cgaccacccc tgacgtcgtc gtcgccgcca accaggagcc ggtcgacccg acccagctcg
    24721 agatcgagct gccgcccggt ctggccggtt cggcctggaa gctgccaccg gtcaagctgc
    24781 tcagcctgtc gggccgtcgc gacatcgaca cccgcgccgt cgcggcccgt ggcgacgtgc
    24841 tggtctcggc gctggccgag cacggcgtgc agacccgtat cgtcggcatg accgtcggcc
    24901 ccacggtcac ccgctacgag ctggagctcg ggaccggcgt caaggtcgcc cgggtcacct
    24961 cgctcaacaa ggacatcgcc tacgcgatgg cgtcgcccga cgtgcggatc ctcgctccga
    25021 tccccggccg tcaggcgatc ggcgtcgagg tgcccaacgc ggaccgcgtc gtggtcaacc
    25081 tgggcgacat cctcacctcc gaggaggccc gccaggccac ccaccccctc caggtcgccg
    25141 tcggtcgcga catcaacggc accgccaagc tgatgaacct ggcgaccatg ccgcacctgc
    25201 tgatcgccgg tcagaccggt gcgggcaagt cgagctgcat caactcgatc atcacgtcgg
    25261 tgatgatgcg ggccacgccc gagcaggtcc ggatgatcct catcgacccc aagcgggtcg
    25321 agctcaccca gtacaaccgg atcccgcacc tgctcaccca ggtggtcgcc aaccccaaga
    25381 aggccgccaa cgccctcaac tgggcggtca aggagatgga gcggcgctac gacctgctct
    25441 ccgaggtcgg cgtccgtgac atcaccgggt acaacaagaa gttcgacaag ggcgagctga
    25501 ccccggagcc gggatcggag cgcacctacg atcgactccc gttcatcctg atcgtcgtcg
    25561 acgagctggc agacctgatg atggtggcgc cccgcgacgt cgaggagtcg atctgccgca
    25621 tcgcgcagat ggcccgtgcg gtcggcctgc acctggtgat cgccacgcag cgcccgtcgg
    25681 tcaacgtgat caccggcgtg atcaaggcca acgtgccggc ccgactggcg ttcgcggtgg
    25741 cctcggccac cgactccaag gtcatcctcg accagggcgg cgccgaacgg ctggtcggtc
    25801 ggggcgacat gctgctgctc gagggcggct ccagcgctcc ggagcggatc cagggggcct
    25861 gggtcgaaga gtccgaggtc cactcgatcg tgtcgtcgtg gcggcgccag gctcccgacg
    25921 tcgaatccca gtacgtctcc gacgtgcagg gatccgatga cgccggaggg tcgacctccg
    25981 gaggatctgg cagcggcgac gacgacgacc tcctggccca ggccatggag ctggtcgtgc
    26041 gcagccagct gggctccacc tccatgctcc agcgcaagct caaggtgggc ttcgcccgtg
    26101 ccgggcgcct catggacctg ctcgaggagc gcggtgtggt cggaccgtcc gagggctcca
    26161 aggcccgcga tgtgctgatg agccccgagg agctcgacaa cctgatcgag tccggggcga
    26221 tctgatcgac ccggacgact ccggtcgctg cggtgggcgg caccgcgacc ccgacgcctt
    26281 cgccgaggcg gtgctgcggg tcgtcgatcg ggtgccaccg ggccgggtca ccacctatgg
    26341 ggccgttgcg gacgccgtgt cggaccggtt cgggggcggt ccccgccagg tcggcgcggt
    26401 catggcccgc gacggcgccg cggtcgcgtg gtggcgggtg gtgcgcgccg acggctcgct
    26461 gccggacccg ctggcggccg aggcccgggc ccgtcaccga caggagggga ctccgctgag
    26521 gtcgtcgggc cgggtggacc tggtcgcggc attctgggag ccctgaccca cccgggtccc
    26581 acacgtcgag gaggagcccg tgccggccaa ggactacacc gcggaggcca tcaccgtgca
    26641 ctggcgttcg gagcgctgca tccactcggc ccgctgcctg cagggactcc cgtcggtgtt
    26701 cgaccgcgac gcccggccgt ggatccgccc cgacggagcg accgccgacc gcgtcgccgc
    26761 ggtggtcgac acgtgtccga gccgtgccct cacctacacc cggaccgacg gctcggcggg
    26821 aggacacaac gccgagcgac ccgacgagga cgcgccgtcg gatgcctcga ccccggtggt
    26881 ggtgaacgtc aagcgcaacg gcccgatggt cgtcgtgggc ccggtgaccg tcgtcgacgc
    26941 cgacggcaac gtcgtgtccc gcgacgatcg gacgttcctc tgccgctgcg ggggatcggc
    27001 ccgcaaaccg ttctgcgacg ggacccacaa gagccgcggc gactgggacg accacaccgc
    27061 ccgctgaccg atcgttccgt cgccctcgcc gggcgacatc gcgccctcgg ccggtcgacg
    27121 ccggtacggt gcgggccgcg gcggccgtcc gggaccgccg ccggaccgag ggggtgcggg
    27181 acatgaccga cgcgacgacc gtgacgacgg tgccgaccga gtggacgacg ctcgcgaccg
    27241 ggttgcgctt cccagaggga cccgtcggct tggacgacgg atcggtgctg ctggtcgaga
    27301 tcgagcgggg cacgctgagc cgggtgtcac ccgacggcac cgtcgacgtc gtcgccgact
    27361 gcggcggcgg acccaacggc gcggcgatcg ggccggacgg ggcggtgtgg attaccaaca
    27421 acggcggctg cttcgagttc gtcgacctgg gcggcatgct cgtacccggc ccggtcccgc
    27481 cgagctggtc cggcggctgc atccagaagg tcgacccgac cagcgggtcc gtgcagaccg
    27541 tgtacaccga gtgcgacggc cgaccgctgc gggcccccaa cgacctggtg ttcgaccgtc
    27601 acggcgggtt ctggttcacc gaccacggcg tacgcctgga gcggtcgagc gaccgcaccg
    27661 gggtcttcta cgccgcggcc gacggctcgt cgatccgcga ggtcctgttc cccctcgacg
    27721 cccccaacgg cgtcggcctg tcgccggcgg gggaccgcct gtacgtcgcc gagacccaca
    27781 ccggccgggt gtgggcgtgg gacgttcccg ggccgggcgt ggcgcagggc agcggcctgc
    27841 tcgaaccgca cggcgacctg ctctgcgggc tgccgggcca ccagctgctc gactccctcg
    27901 ccgtcgacgg cgacgggtgg gtgtgcgtcg gcacgctgct caacggcggc atcaccgcca
    27961 tctcgccgga cgggagccgg ctcgagcatc accccgccga cgacctgatg gtgaccaaca
    28021 tctgcttcgg cgggcccgag ggccgaactg cctacgccac cgcgtcgagc tccggtcgcc
    28081 tgttgaccgc ggagtggcca cgcccgggtc tgcgtctggc gtacccgtga gagcggcgcc
    28141 ccgaggcccg gtcgggtgca gccgatgacc gacagctgga ccgacgaggc caaggtccgg
    28201 gagtacaccg gacgagtagg tcgcctcgcc gcccgccggg cgggcgagat ggagctggtc
    28261 gaggccctgc cggccgaggt cgagcgggtc ctggacctcg gctgcggaga cggccgcctc
    28321 atctcgctcg tcctcgacgc ccggcccggg gtcgcggagg cggtcggtct cgacagctcc
    28381 ccgccgatgc tcgagcgcgc ccgcgatgcg ttcgacggcg atccccgggt gcgcatcgtg
    28441 gcccacgatc tgaggcaccc gctgccggac ctcggccgat tcgacgtggt cgtgtcgggg
    28501 ttcgccatcc accacctcga ccacgaccgc aagcgatcgc tcttctccga ggtggtcgcc
    28561 gccattcgcc ccggcggggt cttcgccaac ctcgaagtgg tccggtgcgc cacaccggag
    28621 ctgcacgagg agttcaaccg gcgcatcggc cgacccggtg gcgaccccga ggacgtgctc
    28681 gccgcagtcg aaccgcaact cgagtggatg cgggtcgccg gtctggagca cgtggactgc
    28741 aactggcgat ggcgcggctt cgcgctgctc gtcggtcggg cccccggcgg cacccccgac
    28801 cgctgacggc ccgggtggag gctcgcgggc gacgctccgc tacggtcgac gctccgctac
    28861 ggtcgccgga tgaccctcct cgcccggctc tggctcgcgc tctggcgttg gtccgtcgca
    28921 cccgccgcgg aacccgtccc ggaccgctgc gtgatgatcg ctgcgcccca cacctccaac
    28981 tgggacttcc cgttgacgct cgctctcgca cgcgtcagcg gggtgaagat ctcctggctg
    29041 ggcaagaagt cgttgttccg ggggccggcg gggccgatca tgcgccggct cggcggcgtt
    29101 ccgatcgacc ggagcgcacc cggcgggatg gtcacctcgc tcgcgaacga gttcgaaggc
    29161 agggaccggc tggtcctggt ggtgccggcg gagggaaccc gctctcgcac cgagcactgg
    29221 aagtccggct tctaccggat cgctcaacag gcagacgtgc cgatcgtgtg cgcgttcgtc
    29281 gaccgacgca cccgcacggg tggcttcggg ccggtcatct cgccgagcga cgacctcgtg
    29341 gccgacatgg atcggatccg tgacttctac tcgggcatgg aggggctgaa gcccggtcgc
    29401 ttcggagaga tccgactgcg ggacgaggac gcgctccccg ccggttgaac gggccgtcgt
    29461 gcccgcaggt ccgatcccgg ggtcgtgggg ccggtgcagg tgcccttgta gcctgcgggc
    29521 gtgtcccgga cgtactgggt cgagaccctc ggctgcccca agaaccaggt cgactccgac
    29581 aagctgatcg gcaccctcca cgccgacggt ctgctggcgg ccgcatcggc cgacgaggcg
    29641 gacctggtgg tggtcaacac ctgcgccttc gtcgccgagg cccgtgagga gagcgtcgcc
    29701 accatcctcg ccctcgagga acgccgcgcc gagggtgccg agctggtggt gaccggttgc
    29761 atggccgagc gctacggcga cgagctcgct gccgctctgc ccgaggtgga cgcggtggcc
    29821 ggcttcggtg ttccggtgac cctcggtccg acccgcggct ccgggcccgc ggtgccgacc
    29881 atggacctgc tcaacctgcc gcggccacgt tcggagcggc cgtgggcgta cgtgaaggtg
    29941 gccgagggct gcgacaaggc gtgcgggttc tgcgccatcc ccagcttccg cggtccgcag
    30001 agatcccggc cgatcgaggc cgtcctcgac gaggtcgcgg cccttcaggt ccgcgaggtc
    30061 gtcctcgtcg cccaggacct ggccgcttac gggcgtgacc gagacaccga cggtcgcatc
    30121 gacgtggcgt ccgggaccac gccgatcgcc gagctcgtgc gcgccgtcgc cgccgaggtc
    30181 gaccgggtgc ggctgctgta cctgtacccg tcggagatga ccgacgagct ggtcgagctc
    30241 atcgccaccg gaccggtgcc ctacttcgat ctgtcgctgc agcacgtgtc ggccccgttg
    30301 gtgcgccgca tgcgtcgctg gggcgacggc gagcgcttcc tcgcccggat cgacgcgatc
    30361 cgcaccctgc gtcccgacgc cgcgttccgg tcgaacttca tcgtcggcta ccccggcgag
    30421 accgaggacg accacgacca gctgctcgac ttcgtcgagt ccgcccgcct cgactggtgc
    30481 ggtttcttcg cctactccgc cgaggacggc acctacgccg ccggcctcga cggcgccgtg
    30541 gagcccgcgc tggtgggtga ccgcctggcc gagctccggg agctgcagga cacgatcacc
    30601 ggccgggccc gagacgcgtt ggtgggggag aggacggtcg tcctcgtcga cgaggtgggc
    30661 gtggcccgtt cgcatcgcga ggcgcccgag atcgacggca tcatcgaggt gcccgacacc
    30721 ctggccgtcg gcgagctgca cctggtcgag atcaccgccg cggccggacc ggacctgacc
    30781 gcggagccgg tgtgagcacg cacccgcccg actcgaccga gcggtcgcgg accttcggac
    30841 cgagcgccct gttgacgccg gcgaacgtca tcaccgtcgt gcggctcctg ctcgctccgg
    30901 tggcgttcgt cctcatctac gagcagcggt cggggtggct gctgttcggg ctgtggatcg
    30961 ccatcaccgc gtcggacagc ctcgacggct acctggcccg acggcagggg accacccggt
    31021 ccggagcgtt cctcgaccct ctggccgaca aggtgctcgc cctcggcggc ctgtgggcga
    31081 tggtgctggc cgggcgcttc tggtggctgc cggtgctgtt gatcacggtg cgcgaggcgc
    31141 tgatctcggc gttccgcagc tactggggcc gcaggggcct ggccgttccc gcgtccaacg
    31201 tcgccaagtt caagacgttc ctgcagttca gctcggtcag cctggtggtg tggccgtgga
    31261 cgaccgggta cacatggatg gccgacacgg tgctgtggct ggccgtcgcc gtggcgtgga
    31321 tcagcgccgc gcagtacgtg caggccgggt cgagggcgac gaccacgatg accggcgact
    31381 gatcgaccgc tcggtcacgg ccggacccgg cgggcgccgg taggttgccc gccatgcgtg
    31441 ttgaggtggt ggccgtcggc accgagctgc tgctcggcca gatcgtggat tcgaactcgt
    31501 cgtggatcgg cgagcagctc gccctcgccg ggctggactc gtacttccag accaaggtgg
    31561 gcgacaacct cacccgcatc gccgaggtga tcgagctcgc cctgtcgcgc agcgacgcgg
    31621 tgatctgcac cgggggcctc ggtccgaccc aggacgacct cacccgcgag gcgatcgccc
    31681 gggtcatggg cgtggagctc gaggagtccg aggaggccgc ggagcgcatc atcgccatgt
    31741 tcggcggccg cggtcgccgg atgtcgatga acaacctgcg ccaggcgatg aagccggtgg
    31801 gcgccgagtt catccccgag cagcccggca ccgctcccgg cctgatctgc ccgctcgagc
    31861 gtccggaccc gctgaccggc gaggccgtcc ccaaggtcat ctacgcggtg ccgggcgtgc
    31921 cgtgggagat gcaggagatg gtgtcgggca ccgtgctggc agacctgcag cgccgggccg
    31981 ggatcacctc gtccatcgcg agccgtaccc tgcgcacgtg gggtgagtcg gagtccggcc
    32041 tggccgagat gctcgccggt cgcatggacg agctcgaccg tctggccgac tcgggagctt
    32101 ccgcggcgac gatcgcgttc ctggcctccg gtggcgaggg tctcaaggtc cgcctcaccg
    32161 ccaaggccgc caccgccgag ggcgtggccg acatcctcgc cgcagaggag caggtggtcc
    32221 gcggtctgct cggcgggctg gtgttcggcg tcgacgacga cacgatggag tccgtcgtgc
    32281 tcgacctgct gcgggagcgt gggatgaccc tcggcgtggc cgagtcgctg accgggggga
    32341 tggtcggctc gcgtctgtgc gacgttccgg gcgccagtga cgtgttccgg gggtcggtcg
    32401 tgtcgtacgc ctccgacgtg aagttcgagc tgctcggcgt gcccgagggt ccggtggtca
    32461 ccgaggacgc ggcccgggcg atggcactcg gggcccgtcg ggtgctgcgc accgacgtcg
    32521 gcatcgcggt gaccggggtc gccggaccgg agcgccagga ggaccgcccg gccgggacgg
    32581 tgtgcctggc ggtcgcgatc ggcgagccgg gctcggccgg ccagggggag tgcctgctcg
    32641 actcgatcga ggtgcgcctg cccggccggc gccgccaggt gcgcgagttc agcgtgatct
    32701 cgctcctcgg cctcctccgc cgccacctcc tcgagatata ggggtcctca acgacacacc
    32761 ttgtcgttct ggacgcatca gatccgaaga atcccgccga aatgcttgag tcgaacactc
    32821 gttcgtaact acgctcctgt ggtgccgggc aggggagtgt cacaccccct cgttacggtc
    32881 agcgcatcaa ctgatcggcg ccgtcgaacg gcccccgaac caccccggcg gcaggtccgc
    32941 cgagcacgaa gggacactca cgtggaacga gagaaggcac tcgagatggc gctcggccag
    33001 atcgagaagc agttcggcaa gggctccgtc atgaagatgg gcgacaaggg caccatggcg
    33061 gtcgagtcga tccccaccgg ggccctggcc ctggacatcg ccctcgggat cggcggtctg
    33121 ccccgcggtc gtgtggtgga gatctacggc ccggagtcct cgggcaagtc cacgctcgcc
    33181 atgcacgtcg tggccgaggc gcagcgcaac ggcggcgtct gcgcctacgt cgacgccgag
    33241 cacgccatgg accccgtcta cgccgccaag atcggcgtcg acgtcgacga gctgctcatc
    33301 tcccagcccg acaccggtga gcaggcgctc gagatctccg acatgctcgt gcgctccggc
    33361 gccatcgacg tgttggtggt cgactcggtc gcggcgttga ccccccgggc cgagatcgag
    33421 ggcgagatgg gcgacagcca cgtcggcctg caggcccggc tcatgtccca ggcgttgcgg
    33481 aagctgaccg ccaacctcaa caagacccag accatctgca tcttcatcaa ccagctgcgg
    33541 gagaagatcg gggtcatgtt cggctcgccg gagaccactc cgggcggccg ggcgctgaag
    33601 ttctactcgt cggttcgcct cgacatccgt cgcatcgagt cgatcaagga cggcgtcgag
    33661 gtcgtcggca accggacccg ggtcaagatc gtcaagaaca aggtggctcc gccgttccgc
    33721 caggccgagt tcgacatcat gtacggcaag ggcatcagcc gtgagggctc cctgctcgac
    33781 atcggggtcg aacacgacat cgtcaagaag tcgggcgctt ggtacaccta cgagggcgag
    33841 cagctcggcc aggggcggga gaacgccaag gcgttcctca ccgagcaccc cgaggtcatg
    33901 gtggagatcc aggatcgcgt gctccgtgcc gtcggcatcg ggcagggcga gtcggaggag
    33961 tcggaatcga ccgatctctc cgatgacgtg gtcgatccgg acgatctccc catcaccctg
    34021 gactgatcgg ccggcggcgt tcccgccgtt cgcgtgtggg atcgacgctt cacgcgtcgg
    34081 tttcacacgc gggactagct gcctgggcgt tcgcgtgtgg gatcgacgcc tcacgcgtcg
    34141 gtttcacacg cggacagcgg tgccgtcttc cccacggttc cgctgtgagc gctgatgagc
    34201 ctccaggatc gacgtgaccc gtcgcgtgtc cgccccccag gacggcgcac gcggcgggtc
    34261 cgtcgcgttc gaatgccgtc agccgctggg cgttccaccc ggcgtcggac ggagcagcag
    34321 caacgccccg atcagcagca cgaacgcacc gacggcgctg ccggcgctga gcatcgccgt
    34381 gcgccgcagg gcgtccaccg tgagctcgac gccatcgacc atcacgaagc ggacatcatc
    34441 ggcgcgggac tggaacatcc acaccatccc gcggtagtcg ccggggccga ccacccggac
    34501 cgatcgcaac gcgagctggt cccactggag tcgtgaccct gtcaccacgc tgatcaaccc
    34561 cgccgatgcg gacaggaggg cgcccaccgt cgcggccacc acccgcccgc ggcgggtccg
    34621 gacggtgagc acgacaccca ccgcggcggt gagcagcgcc atcacaacgg ccagagcgac
    34681 agcgatcgaa cgggcggtgg cggcccagcc ggcgccgccg tcgacgcagc ccggagtggg
    34741 ttcgtttcca cacagcgcag ctgcaggcaa gtagtcgacc cccatgatga acgaggcgat
    34801 cgccgcggtg accgtcagcg cgaccgcagc cagcatcgcc gcggtccaca ccgccgaacc
    34861 ggtcgatcga ccgcgctgag gagcgctcga ggccggtggc gtcgtcgtgg cccggacttc
    34921 ggtgccatcc tccaccggcc gtacgctagc ggcgtgacca agcggtacct ggtgcgcacc
    34981 ttcgggtgcc agatgaacga gcacgactcg gaacgactcg cgggcctgct cgaggccgac
    35041 ggctacgaac ccgccaccga cgtcgaggac gccgatgtgg tcgtcctcaa cacctgctgc
    35101 atccgtgaga acgccgacaa caagctctac ggcaccctcg gccacctgaa gtcggtcaag
    35161 gaccgccgac ccgacatgga gatcatggtc gccgggtgcc tggcacagaa ggacggggac
    35221 ctggtcgccc agagggcccc gtacgtcgac gtggtgctcg gcacccacaa cgtgcatcgg
    35281 gccgtcgagc tgctgcacga ccaccagtcc cacgggcgat ccgtcaccga gatcctctcc
    35341 gagaccgtcg ccgaggacca cgagctgttc ccgtccgccc ttcccgtcgt gcgggagtcc
    35401 ggtcactcgg cgtgggtgac catccagatc ggctgcgaca acagctgcgc gttctgcatc
    35461 gtgcccgccg tgcgcggccc cgagatcagc cgtccgttcg gcgacgtggt cgccgaggtg
    35521 cagcgggcgg tggccgaggg ggtcacccag gtcacgctgc tcggccagaa cgtcaactcg
    35581 ttcggtcgcg acctgacgct ccgattgcgc tcgcagaccg gccaggcgcc cggcggtgtc
    35641 gacgccgcga ccggtcggcc cttggcggat ccggcgcacc ttgccggcgc ccgctgggtg
    35701 gccgaggaca ccgggcgggc ccggcccctc ttcgcggatc tgctccgggc cgtcggcgag
    35761 gtcgacggcc tgcgccgggt ccgcttcacc agcccgcacc ccaaggacct gcggcccgag
    35821 acgatcgcgg ccatggccga ggtgccgacg gtgtgcgagc acctgcacct gccgctgcag
    35881 tcgggaagcg accgggtcct tgccgccatg caccgcggct acaccgccga gcgatacctg
    35941 gagcgcgtcg acgcggcccg gaccggcatc gacgacctgg cgttgaccac cgacatcatc
    36001 gtcgggttcc ccggcgagac cgatgccgac ttcgacgcca ccctcgaggt ggcggccgag
    36061 gcccgctacg acaacgcctt caccttcatc tactcgcccc gtcccggcac cgaggcggcg
    36121 gagctgccac acgcggtgcc ccacgacgtc gccgccgagc gcatggcccg cctgcgaacg
    36181 gtggtcgaac gcacctcccg tctgggccac gaggctcggg tcggacgtgt cgaggaggtc
    36241 atggtcgagg gtcccagccg ccgtgacccc gggcggctca ccggtcggac ccgccacaac
    36301 cggctggtgc acttcccggc cgagccggtg ctgcgcgccg gcacctacgc ccgggtccgg
    36361 gtgaccgcgg cgtcgaccac gaacctcctc ggcgagctcg tcgaggtcgt cgccgagccg
    36421 acccatcgtc gacgcatccc ggtgagcgtg gcgtgagcgg tacgccggcg ggcgatctgc
    36481 cggcaccacg ggccgagccc gacgcccccg gcgtgctcac gcccgatgac ctcccaccgg
    36541 atcccggcgc actcgtcgac ctgttggccc gggcgctggc acgccgtcac gaccggctcc
    36601 cgcccgaccc gggtcgggac ggtcccgaac tcgacccctc cgatgctctc gatcgggccc
    36661 gtcacgccgt ggcgtcaggt tggacgccac actcggggtc gccgttcgag cgggtcgagc
    36721 cggcccggct cctcgacgcc ctggatgcgg gaatcgagcg ggtccgcgcc cggtccaatc
    36781 caccggtgcc gaccatcggc cgggcccgct tctccaacct ggcgctcccg cgctccgaca
    36841 ttgcggagcc gctggtggcc accgcggcga acatccggct gcaccggcgg agcgcaggcc
    36901 gaccgtcggc gccgttccgg ctcgaggacc gggccgccgt cgccgatccg caccgcgacc
    36961 tggccgtcgc cgccgcggac gtcgtgtcca ccttcggtcc cggggcggtc cccgcgttcc
    37021 tcgacgccta ctcccggact cgccgccgcg accagggagt cgaggtggtc gacccgatcc
    37081 tcctcgactg gtggtcgatg atcgccgcca tcgtggaggg cgaccgggat catcacgtcg
    37141 gtgaggaacc atgacaccgg ccgagcaccc tcctggccgc cacctggtca tggtcggacc
    37201 caccgcgtcg ggcaagtcgg cggtcgcggt cgctctcgcc cgactccgca cgtccgtcgg
    37261 tcgccccacc gagatcgtga gctgcgactc gatggcggtc taccggggca tggacatcgg
    37321 aacggccact ccgaccgagt ccgagcgagg cggcgtgccg caccacctgc tgaacgtgat
    37381 cgacccgtcc gacgagttct cggtcaagga gttcgtcggc ctggtcgacg acgcactcgg
    37441 cgacatcgcg tcacggggag cctcagcggt tctggtcggc gggaccggcc tgtacgtcca
    37501 agccgtggtc gaccggttcg acctccccgg ccgctatccc gaggtggcgg cccaactgga
    37561 gctgctcggc acagaggagc tcgccgcccg cctcgaggct ctcgacccgc tcgccgcgtc
    37621 ccgggtgccg ccgggcaacc gacgccgtct cgtgcgggcg ctcgaggtca cgatcggctc
    37681 cggtcggccg ttctcggcgt cggggcccgg aatggacgcc tatccgccca cgccgttcgt
    37741 gctggtcggc ctcaccgtcc cccgggacga cctcggcccg cgcatcgctg agcgctaccg
    37801 ccggcagatg gacgcgggat tcctcgacga ggtggcggcg ctggatgccg gagcgggttg
    37861 gtcacgcacc gcacgcgagg cgctcggcta ccgggagctc gccgcccacc ggcgcggcga
    37921 gtgcaccctc gacgaggcga tcgacaccgc ggtgacccgg acccggcggt tcgcggtgcg
    37981 ccagcagcgg tggttcggcc gcgacccccg gatccgctgg ttcgacgccc cgcgtcccgg
    38041 cgcgcgcgac ggcggggacg gagcatcggt gcagcgttgc gccgaggacc tgctcgggta
    38101 ctggtcacaa gtcgctgcag ccggctccgg ccccgccgct accgtggacc catcgtgttg
    38161 cgactgacca agttccacgg cctcggcaac gacttcctga tcgccctggc cgccgatcac
    38221 cccgacgccg agcatcctct gacccccgat cccgacgtcg cccgcgccct gtgcgcccgc
    38281 cacaccggca tcggtgccga cggtctcatc tacggcctgg ctccgggtgg tgacgaggcg
    38341 gacgtccaca tggtgctgct caacgccgac ggctccgagg ccgagatctc cggcaacggg
    38401 atccgttgcc tggcccaggc ggtgctgcgc cacgagggtc gcgacgaggg agacgtcctg
    38461 gtcgagaccg ccggaggtgt ccgccacctg cgcaccgtga agggcagcgt ggatggtgag
    38521 ctgtggatcc aggtcgacat gggggagccg gccgacggtc ccgtgccgag cgccgaggcc
    38581 caggaggtcc cggcccgggc ccgggccacg gtcgacatcg gcaacccgca cctggtgctg
    38641 cgtgtcgacg acctcgacgg cctcgacctg agcgaggtcg gtccccgcgt cgagaggagc
    38701 ttcgacgccg ggatcaacgt ccacgccgtg gtggtggact cggctcagga cctgcgtctt
    38761 cgggtgtggg agcgcggggt cggactcacc caggcctgcg ggtcgggggc ggtcgcggcc
    38821 gcggtcgccg cccatcggtg gggtgaggtc ggcccgcacg tccgggtgca catgcccggc
    38881 ggcgaggcgg tggtcgacct cgacgggcca cgggcgcttc tcaccggccc gtcgacgttc
    38941 gtggccgagg tggtggtccc gtgaccgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39061 nnnnnnnnnc ggcgagtcgc accgcggcgc cttcggcgag ttcggcggtg agtccggggc
    39121 gttcatcgaa cgaaccttcc gggagcggat cgtgctggtc ggagtcgccc tccccggtgt
    39181 tgccgacgac gccctcgacg agtccctcga cgagctcgag ctgttggtcg acaccgcagg
    39241 tgccgacgtc gtcggccgcg tcgtgcagcg gcggtccgcg ccggatccgg ccacctacgt
    39301 cgggtccgga aaggccgagg agatccgccg cgtcgccgag gcgaccgact gcgacaccgt
    39361 cgtgttcgac gacgagctgt cacccgccca gcagcgcaac ctcgagaagc tgctcgggcg
    39421 gaccgccatc gaccgcaccg cggtgatcct cgacatcttc ggccagaacg cccacagcca
    39481 ggagggcaag gcccaggtgc agctcgccat gttccgctac cggctgcccc ggctgcgggg
    39541 tcggggcgga tcgctgtccc agcaggccgg cggtgtgggc acccggcggg gccccggaga
    39601 gacgcagctc gagatcgacc gccgacgcat cacccgccag gtccacaagc tggaggccga
    39661 cctgcgtcgg atcgccggga cccgggccac ccaacgcaag tcccggcgcc gctccggact
    39721 gcccgaggtg gtgatcgtcg gctacaccaa tgccggcaag tcgaccctgc tcaaccgtct
    39781 gagcgggtcc ggagtgctcg tcgaggaccg gctcttcgcc accctcgacg ccaccacccg
    39841 ccgcctgcag ctgcccggcg gcgagtccgt cctgctgacc gacaccgtcg gcttcgtgcg
    39901 caaactgccc caccagctgg tcgaggcgtt ccgttccacc ctcgacgtcg ccaaccaggc
    39961 cgacctgctc gtccacgtcg tcgacggggc cgcggtcgat cccgacggga acatcgacgc
    40021 cgtgcgcacg gtcctggccg agatcgcccc ggacggcgac ccgtcccgca tccgaccgga
    40081 gctcctggtg ttcaacaagg ccgaccgggg accgggagcg gatcgctgct cggcccgaca
    40141 tccgggctcg gtggtgatct ccgcgaccac cggccagggg atcgacgacc tgctgctgcg
    40201 catcggtgat cggctccgcg ccctgctgcc ggcggtcgag ctgttcgtcc cctacgagcg
    40261 cagcgacgtg atcgccgcgc tgcaccgggc cggcgaggtc atcaccgaga cgcccaccga
    40321 cgacggggtg atgatctcgg cgcggatcgc cgaggacagc gccccccgat tcgccgagtt
    40381 cgtcctggac cgcgcctcgt gagcacatcc accgcgggcg gcttcgtgcc gccgccctac
    40441 ccgtacgacc gcctggccga catcgtcgcg gtggccgccg cacacgacgg cggcgccgtc
    40501 gacctgtcga tcggaacccc tggcgacccc ccggccccgg cggtgctcga cgctctcgcg
    40561 tcctcgggta ccgaacgggg ctatccgccc tcgatcggca ccgaggcgct gcgcgccgcg
    40621 gtggccgagt ggttcgaccg acgcttcggt gtcggcgtcg acccctccga tgtcggcgca
    40681 gcgatcggca ccaaggagct cgtggccggg ctcccgcact ggctgcggct ccgcgacccg
    40741 tcccgtgaca ccgtcctcta ccccgaggtg agctacccga gctacgcgat gggcgccacc
    40801 ctggccggct gccgggcggt cccggtgccg atggacgacc gctggcgcct cgacctgtcc
    40861 cacatcgacc ccgcggacgc ggcccgggcg ctgtgcctgt gggcgaacac ccccgggaac
    40921 cccgccggtg ggctcgacga cctggccgcc gtggccgagt gggggcgccg ccaccgggtc
    40981 ccggtgttca gcgacgagtg ctacgtcgag ttcacctgga gcggaccgcc ggcccgcccc
    41041 ggtcaggctc cgggtaccac ggtgctctcg tcgggaaccg agggcgtcgt ggcggtgcac
    41101 tccctgtcga agcggtcgaa cctggccggg ttgcgggtcg gcttctacgc cggtgatccc
    41161 gaactggtgc actacctcca ggaggtccgc aagcacgccg gcttcatggt ccccggtccg
    41221 gcccaggccg ccgcagtcgt cgccgtcggc gatcagcgcg acgtcgacgt gcagcgggag
    41281 cgctaccgga cgcgcctcgc gcgcatggcc gggatcctgg agggggtcgg gatcgccgca
    41341 ccgctccccg acggaggctt ctacct
//
LOCUS       DAOQOD010000007        40971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_291_length_40971_cov_11.914972, whole genome shotgun sequence.
ACCESSION   DAOQOD010000007 DAOQOD010000000
VERSION     DAOQOD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 40971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40971
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_40971_cov_11.914972"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..715)
                     /locus_tag="PLZ72_01770"
     CDS             complement(152..715)
                     /locus_tag="PLZ72_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HNA35052.1"
                     /translation="MLLWPGAGSDAEHPGLRAVEAGVAPLRVARRDFPYRREGRRAPD
                     RAPKLMAAVRAEVDELCRRWGTGPEHLVLGGRSMGGRICSMVAAGADGEGPLPVAGLV
                     LMSYPLHPPGRPDRLRTEHLPQLRVPVLFISGDRDTFGTPEELAEAQTLIPGPVRSVT
                     LAGAGHDLKRSDDDVVAAIRDWLRPPG"
     gene            complement(755..1462)
                     /locus_tag="PLZ72_01775"
     CDS             complement(755..1462)
                     /locus_tag="PLZ72_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35053.1"
                     /translation="MILAELEAYQSRPIAPTRRLALGELNLPCDPAPGFGGILLGAVA
                     ARFTPDLDEDTAAEVTQLLSDVERGRHVAQPRARHRLQTDRIGLRPCHHRLVGRGESL
                     HLEIDDTRGTPAQHVLAAVYATGTVAAPLRPAVVAAVRKGMRWSGPIGSPLIAHLVGR
                     GRDLPVSAAADPVVWALRVLRLVTDRSRHAPPSRRDVQQAFRDQLRAAHPDHGADDDV
                     AAERIADLAEARRILLG"
     gene            complement(1493..2251)
                     /locus_tag="PLZ72_01780"
     CDS             complement(1493..2251)
                     /locus_tag="PLZ72_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35054.1"
                     /translation="MRDDETTDDEMTDDETTDDGTGDELLYDCTTWAGESRGLLASLL
                     DSHGIPHAWQGTVLSVHPEDEDDVDDLIDDVMASARPALDAAAAKVVYEVGSWPAALQ
                     TMLADSLTVADLPYEWDHNGDLVVYAEHEEDVEAILDEMPDPDDPDLVGDVSADDGIA
                     VHELLDRLFLASGKLASRDDAASVLAVDDVVGTLERMGPPFGFEAPQWRHLVGRSVVL
                     RDALAAAPGAEDSLDDDELRVVAGDVRDLLRTYV"
     gene            2348..3316
                     /gene="glpX"
                     /locus_tag="PLZ72_01785"
     CDS             2348..3316
                     /gene="glpX"
                     /locus_tag="PLZ72_01785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNA35055.1"
                     /translation="MTNTAPDRNLAMELVRTTEAAAMAAAKWVGRGDKEGADGAAVDA
                     MRLILGTVSMTGTVIIGEGEKDEAPMLFNGEVVGDGSNPQVDIAVDPIDGTTLTSKGR
                     GGAVAVIAVSEQGTMFDPGPCVYMEKIAVGPDVAGHVDINASVSDNLHAVARAKGVDV
                     REVTATILDRPRHDDLVAEVRETGSRIRLIGDGDVAAAIATAYPDSGVDILFGIGGTP
                     EGVIAAAALKCMGGELIGKLWPRDAGERDAAIAAGYDLDKVLGTDDLVAGDNCFFSAT
                     GITDGNLLQGVHYDRLGVTTQSLVMRSRSGTVRLVEARHRPGKSEA"
     gene            3332..4246
                     /locus_tag="PLZ72_01790"
     CDS             3332..4246
                     /locus_tag="PLZ72_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNA35056.1"
                     /translation="MEFDDDARLDTSHIDDARGRGGAGPDLGGLPGGFGTGAKLGGGG
                     LIGLLVVIVIAFLSTRGGGFATNDMLEPSTTVGDQSATEVARECRTGADANERQDCRI
                     VAVVNSVQRYWSDTFARSGVTYRPARTQLFSNRTYSGCGPASSATGPFYCPADETVYV
                     DLTFFDALSQPPFNAAGGPFAQAYVIGHEYGHHVQNLLGRSAGADRQGATSGSVRLEL
                     QADCYAGVWAANAVSTGFVTEITPEDIAQGLDAAAAVGDDRIQESAGGRSNPDSYTHG
                     TSAQRQRWFQRGYDTADPTACDTFSGRI"
     gene            complement(4251..5198)
                     /locus_tag="PLZ72_01795"
     CDS             complement(4251..5198)
                     /locus_tag="PLZ72_01795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA35057.1"
                     /translation="MARRRLAVVLLAPQAEAAEIDALRRAVGLVEPFHVAPHVTLVPP
                     TNVAEPDVPDVLGVLRRVAATSRPLELEVGPAATFLPETPTLHLAVAAADPEALSSLR
                     GRLRCGPFDRPDVWPFHPHATIRESAEPELIAAAVTALAGLIQRWSVASVHLLEQRRQ
                     GPDHPRHGQAHWVAIREEPLGGPAVVGRGGIELALRTVSMVEPEVAGLLGTDPTEPPS
                     RPGPRPLVVVAEDPGTPGPVVGAALGSVGNGSTAAEVVSVGVAEDRRGEGIGRQLVNA
                     WCSEAARRGAEVAVGPDHPSLVAVGFEPAAGVAVRSLGR"
     gene            complement(5277..5687)
                     /gene="atpC"
                     /locus_tag="PLZ72_01800"
     CDS             complement(5277..5687)
                     /gene="atpC"
                     /locus_tag="PLZ72_01800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNA35058.1"
                     /translation="MALEVELVSPERIAYSGEARMVICRTTTGDIAFLPGHIPFIGVL
                     ATHPVRLQLEDGSEKVIAVHQGFVEVSPPDEQGNTRVTILSDVAEMAADIDVDRARTA
                     KERAEEALRADSEDPEAKAALVRAEVRLTAAEAA"
     gene            complement(5687..7111)
                     /gene="atpD"
                     /locus_tag="PLZ72_01805"
     CDS             complement(5687..7111)
                     /gene="atpD"
                     /locus_tag="PLZ72_01805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNA35059.1"
                     /translation="MTDGRIVAIAGPVVDVEFPSDSVPEINQALEFTITVDGVDTVVV
                     AEVAQQIGDNRVRAIAMKPTDGLVRGTAVRNLGHGISVPVGDATLGHVFNVLGQPLDV
                     ESVDSPERWAIHREAPAFDTLEPKAQMFATGIKVIDLLTPYLQGGKIGLFGGAGVGKT
                     VLITEMINRVASQFGGVSVFAGVGERTREGTDLLIEMGETALGESGSVLDKAALVFGQ
                     MDEPPGVRLRVALSALTMAEYFRDVQGQDVLLFIDNIFRFTQAGSEVSTLLGRMPAAV
                     GYQPTLADEMGELQERITSTGGRSITSLQAVYVPADDYTDPAPFTAFTHFDGTTELSR
                     DIASLGIYPAVDPLASSSSILTPEVVGERHYAVARRVQEMLQRYKELQDIIAILGIDE
                     LSEEDRVTVDRARKIQKFLAQPMFVAEVFTGLPGIFTPVEDTVESFEMLVNGDLDHLP
                     EQAFFNVGGAEDAMRKAKELEANA"
     gene            complement(7211..8194)
                     /locus_tag="PLZ72_01810"
     CDS             complement(7211..8194)
                     /locus_tag="PLZ72_01810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNA35060.1"
                     /translation="MAGAQERVLRRRIASVQNTKKITRAMELIAATRVVKAQQRANEA
                     RPYAEHITQVIQDLSAAGAEVDHPLLRPLGVVEKVGVVVLAADRGLAGAYNSSVIKAA
                     EREVQAARSEGKEYALVTVGRKPKAYFSFRHYPIEASFEGFSENPSFEDAVAVAARVR
                     DLYENGGCDRIDLVYTRFVSLGSQEVVVRRFLPLEDADTIAEGGDASATAGFEFEPSA
                     AAVLQTLLPRYVESRLFAALLDAAASELANRQRAMKSATDNAEELIVKYTRKMNQVRQ
                     DAITTEIMEIIGGAEALADDREDPGELLLHHLDTEPFLPLDHRHQSGPVRT"
     gene            complement(8220..9860)
                     /gene="atpA"
                     /locus_tag="PLZ72_01815"
     CDS             complement(8220..9860)
                     /gene="atpA"
                     /locus_tag="PLZ72_01815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNA35061.1"
                     /translation="MAELTINTADITAAIKKNLEGFAPDVELTQVGRVIEVGDGIARV
                     AGLPDAAVNELLEFESGLFGLALNLDEDSIGAVVLGEVDSIEEGQTVKATGNILSVPV
                     GDGVLGRVVNTLGEPIDGKGPLTNVQDRRMEIQAPGIMGRKPVGEPMQTGIKAIDSLI
                     PIGRGQRELIIGDRKTGKTTIAIDTILNQQGLGVKCIYVAIGQKASTVAQTVATLAER
                     GAMEYTVVVVAPASDPAPFKYLAPYAGCAMGQHWMENGEHALVVYDDLSKQAEAYRQV
                     SLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDERGGGSLTALPVIETKAGDVSAYIP
                     TNVISITDGQIFLQDDLFKSGVRPAVDVGISVSRVGSAAQIKAMKTAVGTLKSDLQQF
                     RELEAFAAFGSDLDAVSKAQLERGYRLTELLKQGINSPMSVQDQCVVIFAGTRGYLDA
                     IAVEDVRSFEDGLLDWFRTRHAGMLSAIVSTGKIDDEDAFDAAVKAFAAQFQGTSVDS
                     SAPAAQAQADAESTIATSSRHLPEEDVTRDGDDDAATV"
     gene            complement(9862..10389)
                     /gene="atpH"
                     /locus_tag="PLZ72_01820"
     CDS             complement(9862..10389)
                     /gene="atpH"
                     /locus_tag="PLZ72_01820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNA35062.1"
                     /translation="MSDAQISAYADAAYGIAAAEGNIGEVEDELFRLGRVLQTNDELR
                     GTLTDAHLPVERRAQIVEDLLDGKATRTTVSFVSMLVSAGRIGDLAAIAEAMAQRSAA
                     SRGEAVAEVRSAVPLTDAQLADLARALKARTNIDVTIRNVVDPTVMGGVVTQIGDSVL
                     DGSVRTRLNQLREAF"
     gene            complement(10433..10984)
                     /gene="atpF"
                     /locus_tag="PLZ72_01825"
     CDS             complement(10433..10984)
                     /gene="atpF"
                     /locus_tag="PLZ72_01825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNA35063.1"
                     /translation="MNLLHSIVSPALLAAEGSNGKWLPADINEVIWGSLAFLIVATLL
                     VKFGKAPIVNGLKARSDRIAEHLGEAAAAREAAEAERDRIKAALADSDSEAARIVEDA
                     RRAADQLGIDTAARTETEIAALRERAGADLAATRMQATADLSGELSRLALGAAEEVVA
                     SSLDDATQQRLIDDYISQVGAQN"
     gene            complement(11002..11541)
                     /gene="atpF"
                     /locus_tag="PLZ72_01830"
     CDS             complement(11002..11541)
                     /gene="atpF"
                     /locus_tag="PLZ72_01830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNA35064.1"
                     /translation="MSVLAAEAAESAEKINPVVPDVVGEIFWGAVFFFLLWILMRYVC
                     LPPLLRVRAQRDAQVQADLEAAEAASTQAEQVRRDYDATLAEARGEASRVLEEARAAA
                     EATRGQKVAGVETEIAAERQAAMAELDAERAAALGQLSGDVADLAVAAASKVVQAPLD
                     AASHRSTVDDYVNRSGGPR"
     gene            complement(11628..11924)
                     /gene="atpE"
                     /locus_tag="PLZ72_01835"
     CDS             complement(11628..11924)
                     /gene="atpE"
                     /locus_tag="PLZ72_01835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNA35065.1"
                     /translation="MSALHHAAGIIAQVGDAAGLDADAAKATAAASGAGMAYGLAAIG
                     PGIGIGYLVGQAVQAMARQPESAGQVQTTMFLGIAFTEALALIGFVVFILLKFV"
     gene            complement(12013..12834)
                     /gene="atpB"
                     /locus_tag="PLZ72_01840"
     CDS             complement(12013..12834)
                     /gene="atpB"
                     /locus_tag="PLZ72_01840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNA35066.1"
                     /translation="MPGLALLNAPSILLAAEDGGGGGMHFPPIDHIVKWPAWFGEGTF
                     YEFNKITFISFIAMLVPVILFLIAGRKSKLVPSGLQNVMEVSIDFVEKQVVLPAIGPE
                     GMRYLPMLISMFFFIWIGNIFEVIPSAHMPANARMANPLVLALVAWAMFIGVGVKHNG
                     LGYFKSALFPPGVPKALYILVTPIELLSTFIIRPFSLAVRLFANMLAGHILLVTFAVL
                     CITLWSASVLAGVLVLSFPMLVAFTGFEFMVAFLQAFIFSLLTAVYIGGALHPAH"
     gene            complement(12850..13386)
                     /locus_tag="PLZ72_01845"
     CDS             complement(12850..13386)
                     /locus_tag="PLZ72_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNA35067.1"
                     /translation="MSVEGKVPADGMLGPRVEGPSPAMAVAFDIVRRSVWAIPIAVIA
                     SAAFWGLDGVFSTLYGLAIVIVNFLLAGWLLQVGGRIGAAAMGAAAMFGFLLRLGLIM
                     VAVLAVRDQPWVELVPLGLTIIITHLGLLFWELRHISSSLAFPGLKPQATPNPYLPVA
                     DDASGPSTSTSAQSPTAP"
     gene            complement(13383..13730)
                     /locus_tag="PLZ72_01850"
     CDS             complement(13383..13730)
                     /locus_tag="PLZ72_01850"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNA35068.1"
                     /translation="MPDHRTSTEPGDGSQTELTRSLHRSAGSFELVFAPVIMALIGLW
                     LDRTLGTAPVFTVGLAVFGAVGAGLAMYFSYHRQLDELVEQGRIPVDRPAAQYHAANR
                     SEPRPVESPEGHR"
     gene            complement(13840..14298)
                     /locus_tag="PLZ72_01855"
     CDS             complement(13840..14298)
                     /locus_tag="PLZ72_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35069.1"
                     /translation="MSNPESTVPGADAARVAQFREEIDSLKLKGGSAEGEQRLLALGV
                     VLMVVGLVLALFGAIQVGLQGDFPAEQRAYMAQGSFLGIALVIAGGALFVRYSLARYL
                     RFWLIRSSYEQRANADRIVEAIERAAAPTAAPAPAVVPPTFTGTAPTSHP"
     gene            complement(14421..15122)
                     /locus_tag="PLZ72_01860"
     CDS             complement(14421..15122)
                     /locus_tag="PLZ72_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35070.1"
                     /translation="MSEPLIELRGVRAAYGTIEVLHGIDLAVMPGQVMALLGPNGAGK
                     TTTLAVASGQLAPTGGQLMVAGRDVTGVSPEDLARRGLTTIPEGKGIFPNLTVRENLV
                     MATYAGVSLSRVEEVAYAQFPRLKERRTQVAGTLSGGEQQMLAMARGLAVEPAVLLLD
                     ELSMGLAPLVVEELYGIVSEIARSGTSILVVEQFARTVLGVADVAAIMVNGTIRQVGR
                     PDEVERELSAAYLGG"
     gene            complement(15119..15967)
                     /locus_tag="PLZ72_01865"
     CDS             complement(15119..15967)
                     /locus_tag="PLZ72_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35071.1"
                     /translation="MSSLEVTDLSVRFGGHLAVGGVSLRVDGGRICGLIGPNGAGKTT
                     TFNAICGVITPTSGRVVLDGRDVSKLSTHQRARVGIGRTFQRLEVFSSLTVRDNVRVG
                     LEIRKGWSRRRAPAPAFLAGGADISPVDEVELILQRLQLTELADLPVGSLPTGQARLV
                     ELGRALAARPRVLLLDEPASGLDERETVDFGNLLVDLAGAGLGILLVEHDISLVMRVC
                     EHLSVLDFGQVIAAGDPVAIRSNEAVLAAYLGAPAGDDELVGAAAIAEAAGHANGHGP
                     GRGGAS"
     gene            complement(15964..18336)
                     /locus_tag="PLZ72_01870"
     CDS             complement(15964..18336)
                     /locus_tag="PLZ72_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35072.1"
                     /translation="MDKFLTFTIAGLSTAAIYAIAASGLVVTYTTSGIFNFAHGAFGM
                     LAAFAYWQVNQGWGVPVIPSVILVVFVMAPLFGAVIERGIMRGIEGASEVVKIVVTVS
                     LMVALLGIANVIWPPDVARPVPPFFAGKTVEIAGVAVPYSRILTMAIAIAVAVGLRLV
                     LRRTRLGVAMRAIVDDRALLQLNGGRPGRTAMLAWALGAGLAAVSGVLIASEQTLSAV
                     TLTLLVVNAYAVAVVGRLRSLPGAFLGAVVLGLAESYATGYIDPSYALGPVSLQNLRT
                     AIPAVMLFVVILVQPEARLRAHGVSRPRSPVRPPSQVTAIWGGIALVLVAAAVGSLLS
                     APDLNLVSKGFGFALITLSLVPLTGYAGQISLAQMTFTGIGALAMATWGATGSPVGVA
                     IAVVLCAVVGAVVALPALRLSGIYLALATAAFALFCTSMVFGQSEVMPGGSIQVPGLS
                     IGGFEVTNDYRQLILFAVTFALLGNLLVLLRRSAWGRRVAAMKDSPVACATLGLNLTS
                     TKVGVFALSAAIAGLGGALAGRTFIADDFGLTNSLPITMLAVVGGIGSVAGAFLGGLL
                     LGAIPIASTIFAANAIGIFRFVSMPVTDVLSFTPGLMGISLGQEPDGAAPQMAAGYRA
                     VGESVPSLVIAGVGGVGLWFATRAGVITKWSFFAALLVFVVAVVPLLPILFAPDTPEG
                     PRPRRAATGIWLALGLLVAAFLPWADRISSNGWRILGIGVFTFLVLRVAIGVMGVVPE
                     SLKKVEHPSPDELGLDRPLSRADALDAGRSLGLDEELMPSAVGAGIGGAP"
     gene            complement(18355..19785)
                     /locus_tag="PLZ72_01875"
     CDS             complement(18355..19785)
                     /locus_tag="PLZ72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA35073.1"
                     /translation="MARSTHIRHVGVALLATMALVLGACGASGGDEKGSDKEGSTTTA
                     AGSSTGSEWGTLPSPCGKGSFKVAEGEGGTGTDKLYIGVPNDRGAAIRPGLNKELWDA
                     SVAFTDWCNEQGGIGGLQIEPVDLDGKLFEVEAAMAKACHSVFGFVGGAFTQDDLSFT
                     GKEGSDFHKCGLIDVPAFAVSVQKSLSNGQVQPIPNPPNKKSEQWIMDFKKLYPEQSA
                     KNVVVYGELPSLKVVKLQYDAAVQAVGGIQQLEALSYPVAGMADWTPLAQKVIDSGAG
                     SLYWIGEPGNAANLMSKLKEQGWKGVALNETNVYDRTYFSQGPAAVDGSVVRLGFHPF
                     EEADKWPAIKQYQDNLKKYVPDGKEAALGLQSTSAWLLFATAAKDCGDQNKGVISRDC
                     VLDRAAEQKDWTAGGTHVPTDPGGDEPPECGMLMVVKGDGFERLWPEIGGEDDDQDGF
                     HCPPDSIATVTAELPAKGAIDPDRKR"
     gene            20065..21795
                     /locus_tag="PLZ72_01880"
     CDS             20065..21795
                     /locus_tag="PLZ72_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA35074.1"
                     /translation="MDLAIRNVTIVDGTGAPARVGDVGISNGRIVTVAETADPALDGA
                     ERTVDGTGLMLCPGFVDPHTHYDAQLLWDPTASPSSVHGVTTVIAGNCGFTLAPLNPG
                     DGDYVRRMMAKVEGMPLAALEEGIDWDWETFADYLARFDGNLAVNAGFLVGHCALRRK
                     VMGTDAVGSQPTDEQLRDMEELLAESIRAGGLGFSTTRARTHSDGDGEPVASRWAEEP
                     ELLALAEVVSQHPGTTLEFASDGCLDGFDDDEVQFMIRFSRAGNRPLNWNVLTVDSHA
                     PERYRNQLAAMDTAAAAGARVVALTMPVIVGMNMSFLTYCALNMMPDWGDILALPVPE
                     RIEKLSDPGTRRFMEERAASPDAGVFARLTGWSTYVIGDTFSEANEGLRGRRVGDIAA
                     ERGTGAFDTLLDIVIADDLRTVLWPGATDADPESWELRRQAWQHPSVMLGGSDAGAHL
                     DRMQGANYPTRFLADCLRGRKLTTVEDAVRMMTTVPAELFGLRDRGVIREGAHADLVL
                     FDPDVVDSEMLSMVDDLPGGTERLFAGSVGVRSVLVSGVEVVRDGRPTEARPGTIIRS
                     GVDTDTVTAS"
     gene            complement(21820..22920)
                     /locus_tag="PLZ72_01885"
     CDS             complement(21820..22920)
                     /locus_tag="PLZ72_01885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNA35075.1"
                     /translation="MRDYLIVMAVSVAATAASVPPLRSLSRRVGAMAEPSDRSVHAEP
                     TPLLGGAALFVGLVVGIATAALLPGLRPVFEAPSNVLGVLLAAAVMFVTGLVDDLRDV
                     SPPAKLAGMVLSGSVLTLVGLTVIYLRIPFLGFTVLPPDLSALVSVLWVVGLANAVNL
                     IDGLDGLAAGIVAIASFAFFLYGWQLFQVGVVDVTNVGLLIAIVTAGVCLGFLPFNFN
                     PASIFMGDSGALLLGLLLASSTIAVGGQSDDPFSGQSWFFFAPLVIPLVILGVPLLDT
                     AFAVIRRASRRSGVATADKAHLHHRLMDLGHGHRRAVAILWAWTALLSAFVLVPVYTD
                     RGNGIVPIGLLALGLLLFTAFAPRLGEMRARS"
     gene            23207..24790
                     /locus_tag="PLZ72_01890"
     CDS             23207..24790
                     /locus_tag="PLZ72_01890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA35076.1"
                     /translation="MQSEWGHATRAQDLRRRIGSADVFARGAVSMDAPSEVSVPELTI
                     VGDPLPRTRRVLGRVPFPVQVKTALVAADLVATICGMAMAAGLSARVGVTRGETGATV
                     LTALLSVPIWPLLYAHQGLYQARRITRRADEFRGIVNSVLAGVLVLAGISVILQQEHL
                     REWLLITAACVTGTVTLERELARRVIGEFRSRGVMTRRVVIVGRNEEATELAEALHAN
                     RGLGYEVVGFVADVPVGPVIDLRSDPELGPYLGRTAEVLDLVRATRAGGVIVATTGVD
                     QRSANRLIRDLTREGLFVELTSSMRDIAANRISVRPLGPYPVMCVEPVAQFSWRRAAK
                     RTFDLIVASVAIVITAPILAAAAIAIRVTSGPGVMFHQERVGKDGVPFRLHKLRTMVP
                     DAEERLADLKALNEASGPMFKMADDPRVTPVGRFLRATSIDELPQLFDVLKGQMSLVG
                     PRPALPCEAAQWDETLWERLRVQPGITGMWQVSGRYTTSLETYARLDLYYVDNWSLVT
                     DLLILARTVGVVLRRKGGA"
     gene            complement(24797..26065)
                     /gene="glyA"
                     /locus_tag="PLZ72_01895"
     CDS             complement(24797..26065)
                     /gene="glyA"
                     /locus_tag="PLZ72_01895"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNA35077.1"
                     /translation="MPWPTQPDTEIFDMIDREVDRQNTGLQLIASENFTSPAVMDAVG
                     SVLTNKYSEGYPTKRYYGGNQVVDQIEDLARQRVCELFGAEAANVQPHSGANANLGVY
                     LALLEAGDTVLGLSLDHGGHLTHGSPVNISGRFYDFVSYGLTPSDERLDLDQLRDLAR
                     THRPKMIVAGATAYPRIIDPAPIREICDEVGALFLFDAAHIAGLIAGSAHPNPVPYAD
                     VVTFTTHKTLRGPRGGCIVSRAEHAAAIDKAIFPGLQGGPLEHVVAGKAVAFREAATA
                     EFGDYARQIVANAVALAGALGERGFRLVSGGTENHLMLIDLRTFDADLTGKDAQNALD
                     DAGITLNKNTVPDDPRSPFVTSGLRIGTPAVTTQGMGETEMVTIAELITRVLRDVGDT
                     AEIAAVRSEVAELCAGFPVYGNDAGRPTTP"
     gene            complement(26124..26576)
                     /gene="rpiB"
                     /locus_tag="PLZ72_01900"
     CDS             complement(26124..26576)
                     /gene="rpiB"
                     /locus_tag="PLZ72_01900"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNA35078.1"
                     /translation="MTERIAIGADHAGVHLKDALGGHLADRGYEVIDLGTHGTDRVDY
                     PDFGAAVGRAVAGGDADLGLCVCGSGIGIAIAANKVPGIRAATVHDQTSARLAREHND
                     ANVICIGERLTGEEVARDALDAWLDAEFQGGRHAGRVAKISELDAGLA"
     gene            complement(26573..26995)
                     /locus_tag="PLZ72_01905"
     CDS             complement(26573..26995)
                     /locus_tag="PLZ72_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNA35079.1"
                     /translation="MTRKSTDDVAWLSSVPFFEGFSNDDLHRVVDLSQQMEATAGTVL
                     VDQGDPGTQCYVIVEGQASVYVRGEHVASSGPGSMVGEMALIDHRPRTATVVADTPMK
                     LLRFGTREFRTLLDEMPKASERVMTVLRERLERSEATS"
     gene            27118..28155
                     /locus_tag="PLZ72_01910"
     CDS             27118..28155
                     /locus_tag="PLZ72_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNA35080.1"
                     /translation="MRAVVMRDGALVVDEVSLPALQPGQVRAEVLACGICGSDLHALH
                     HADRMVEMSELTAEPGGIAPRMMDPSRDVVMGHEFTARVLEVGPNVGNCTPGDVVVSL
                     PVVMDAGGIHAVGYSNDYPGGYGQQIVLSDMLTMKVPDGLDPELAALTEPMAVGLHAV
                     EMSRVSPRHAAVVLGCGPVGLAVVAALALAGVETIVAADFSPARRALAATMGATEVVD
                     PSVEGAVAAWRRVEGRKPLVIFEAVGVEGMLDAAMVDAPRGSQILVVGVCMVPDRIRP
                     MVAVGKELNLQFVLGYDPLQFQTTLNRIAEGSLDVSPMITGRVDLDGVAQAFSALGDP
                     EAHAKILVVPS"
     gene            complement(28189..29130)
                     /gene="prmC"
                     /locus_tag="PLZ72_01915"
     CDS             complement(28189..29130)
                     /gene="prmC"
                     /locus_tag="PLZ72_01915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNA35081.1"
                     /translation="MSGGSSDEDETSTVTWRELLEGAIATLRSRDPGDGSDPATDAAR
                     ITAEALGVADAELVLHLSGRATRRGVAHLDAMVARRATGEPLQYVLGHWPFRHLDLMV
                     DRRVLIPRPETEQVVEVALAELDRIGGRDRPTTVVDLGTGSGAIALAVATERVRTAVW
                     ATDASTDALEVARANLAGVGRAGARVRIAHGEWFDALPEELGGTVDLVVSNPPYVAAN
                     ADLSPSVAEWEPAGALFAGADGLDDLRVILAEAPGWLVPDGVVVCELSPEQGAAAVEL
                     AGEHFDEVFLAQDLTGRDRALVARHPRSVRRFTTSTL"
     gene            complement(29127..30206)
                     /gene="prfA"
                     /locus_tag="PLZ72_01920"
     CDS             complement(29127..30206)
                     /gene="prfA"
                     /locus_tag="PLZ72_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNA35082.1"
                     /translation="MTDGSLLSRAAGLERELADTEARLADPEVVADHRRYTEMSRRYR
                     ALQPVVETAVALRTATDDLAAAEELLAASSGDEREAWRAEATAARESIERLEQELRVL
                     LLPRDPNDDRDVIVELRGAEGGEEANLFARDLMEMYRGFAERQGWTVEVLSASPSDLG
                     GVSEAVLRVAGDGVWTRMKFEAGPHRVQRVPVTESQGRIHTSSATVVVLPEADEVDVD
                     IDPNDLQIDVFRSSGPGGQSVNTTDSAVRITHRPTGLVVSMQDEKSQIQNRAKALRVL
                     RARLLQMEQDKAMAEASGQKRAQIGGGGRSEKVRTYNFKENRVTDHRIGLTVYNLDKV
                     LAGELGTVSDALVADEQARRLAELT"
     gene            complement(30203..31219)
                     /locus_tag="PLZ72_01925"
     CDS             complement(30203..31219)
                     /locus_tag="PLZ72_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35083.1"
                     /translation="MLSDEQGARLRRAQLGALVRDLTGASPEVVSLGTNAAAIDGATA
                     YLLSEVADPASLAGALAWTLRQGAAELVLFTDDLGGDVARMAGWFAVPVQVRSVQGAG
                     SVPVPPAPFPERLDVPDPSDGAVADLFTALTDAGLEVVVEDGVVRGEVLGLEVARLVT
                     WPVESGGDGELHLEAGVGRFDRDAVAAMHHGEPPTTTLARSVEMVRRERVPGAGTHPL
                     SRLGRERWLRCSILEDPALVGATRLQPTETTVRRDSVREASPAAAVGIDAAGGPVVVV
                     CSTGADLAFVPVAADTRAWVDPSARLVLAVPERDRIPTLDRLAAALVSPAEVVAVPAP
                     WEQS"
     gene            complement(31254..31541)
                     /locus_tag="PLZ72_01930"
     CDS             complement(31254..31541)
                     /locus_tag="PLZ72_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015650714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNA35084.1"
                     /translation="MKPGIHPNYRYVVFQDTSSGDKFLTRSTIETTETVTWDDGNEYP
                     LAKVEISAFTHPFFTGQMKIVDTAGRVERFERRYGKRRKAAKADAPADAGE"
     assembly_gap    31687..31786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31825..33585)
                     /gene="rho"
                     /locus_tag="PLZ72_01935"
     CDS             complement(31825..33585)
                     /gene="rho"
                     /locus_tag="PLZ72_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNA35085.1"
                     /translation="MSLDSIDVDGLERKDRAELATIAEALGGKPTSRTKKADIIGMIL
                     DLAGVTSAGPDAAPAAPAAPARNGRNGAAGADTEAAPAEADAEASDGGQGPSDRDRSD
                     EPESKPENEPEGQVDQDQDQGGKSQGGKSQGGKSQGGQNQGGQNKGGQNRNQGGQNQQ
                     NQQNQNQGGQNQGEPEPGNRRRRRRRGRDRDRQGEDAGQPQNDNWDGEPVEVAGFLDL
                     RDEGYGFLRVKGFLPSRDDVYVSVKQTRQLGLRKGDFLEGASRPAGRGEKNPALLRID
                     KVNGGDPEKARERPRFEDLTPLFPDERLRLEREDLPGDMTSRIVDLIAPIGKGQRGLI
                     VSPPKAGKTTVMKTIAQSIEHNNPEVHLMVLLVDERPEEVTDMRRSVRGDVIASTFDR
                     PSDEHTQVAELASERAKRMVEMGKDVVIILDGITRLARAYNLAAPATGRIMSGGIDTG
                     ALYPPKKFFGAARNVEEGGSLTILATALVETGSKMDEVIFEEFKGTGNMELRLDRKAA
                     EKRIYPAIDVDASSTRHEELLFERTQLQQVWKLRRVLSGLAAEGNTGAGLELLIDRMR
                     TFPNNDVFLAEIAKAPGVPG"
     gene            complement(33858..35027)
                     /gene="apgM"
                     /locus_tag="PLZ72_01940"
     CDS             complement(33858..35027)
                     /gene="apgM"
                     /locus_tag="PLZ72_01940"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNA35086.1"
                     /translation="MKYVVCVPDGCADEPFEGLGGRTPLEVAHTPTIDALAARGTVGR
                     AGVIPEGMAPGSDVGNMSILGYDPAEFHTGRAPIEAASLGLKLPADRLAYRCNLVTVG
                     DDATMVDFAGGHPSTDAARAVLEDLDATLGRGNPDSVLEFHAGVEYRHIMVAPADWLD
                     AECVPPHDLTGRPAVAPSGPAAERLIEVTEASRAVLARHPDVAANQIWLWGQGFQPSM
                     PDFAAHHGITGGITTAVDLVRGLGVLTDMALPDVEGATPGYDTDYAAQRDAALAGLAD
                     GLDLFVIHVEATDEAGHAGDAAEKVRSLELWDSQILAGLVPELDRMGPWRLLMLPDHA
                     TPLARRTHTPDPVPYLLVDSAVDGPGGTYTEAGVADTPVVPGHELLGRLIGAGAA"
     gene            complement(35229..36686)
                     /gene="thrC"
                     /locus_tag="PLZ72_01945"
     CDS             complement(35229..36686)
                     /gene="thrC"
                     /locus_tag="PLZ72_01945"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNA35087.1"
                     /translation="MRYVSTRGAAPVLGFSDVLLAGLADDGGLYLPERWPELPPLDEL
                     RGLSYGEVATRVMWPFVDGDVDAEGRPVPGALDRESFESIVAEAYATFDTPDVCPVVP
                     LGSTGGETGPDLHLLELFRGPTLAFKDVALQLVGRLFDHVLSARGERVCVLGATSGDT
                     GSAAMHACADRDCVDIVILFPDGRTSEVQRRQMTTLGAANAHAVAVPGTFDDCQDLVK
                     AAFADEAFRDEVHLSAVNSINWARVMAQVVYYVTAHLAVAPDGGPVSFVVPTGNFGNI
                     LAGWVAKRMGLPIERLVVASNRNDILTRFFSTGTMAITEVVPTTSPSMDIQVSSNLER
                     LLFEVLGRDGVALAELMARFRADGSVSVPSEVLAELRSEFDCGRLDDESAAAVIRSVQ
                     QHTGLLVDPHTAVGIGVAEALAGAGVLTDPSVPVVCLATAHPAKFPDAVEAAVGVRPA
                     LPDALGDLFEREERVEHCPADLASVQALVRRAIAG"
     gene            complement(36699..38015)
                     /locus_tag="PLZ72_01950"
     CDS             complement(36699..38015)
                     /locus_tag="PLZ72_01950"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNA35088.1"
                     /translation="MSERVAVSEGTVNIGLLGCGNVGAALVELVRDRGDEIAARTGLR
                     LAVTRVAVRSLSKERGVELPAEVLTTDAESVAADPDVDLVVEVIGGIEPARELIVDAL
                     KAGKPVVTANKELLANVGGELYAVAESSGVDLLFEAAVAGGIPLIRPLRESLVGEDVA
                     RVMGIVNGTTNYILTKMTEEGWSYAEALAEAQSLGYAERDPTADVEGFDAGAKAAIIA
                     TIAFGAKVVAGDVFHEGISGVTSSDIDHARRLGYQIKLLAIAERYGDGSVAVRVHPAM
                     VPDAHPLASVRDSFNAVFVEGEAVGDLMFYGRGAGGFPTASAVLGDVIDAAANLAKGT
                     HASLGSFGKARIRPIDELRSAFYLNLVVEDRPGVLAAVAGVFGAHGVSIRSMEQESLD
                     SGDSGARLTFITHTAREADLRSTLGELRGLDAVRSVGSVLRVIGSD"
     gene            complement(38064..39350)
                     /gene="lysA"
                     /locus_tag="PLZ72_01955"
     CDS             complement(38064..39350)
                     /gene="lysA"
                     /locus_tag="PLZ72_01955"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNA35089.1"
                     /translation="MSAPTNSPLPFHLLPDTATVDDHGRLSIGGCDVADLADRHGTPL
                     FVYDEAHIRARCREAVSVLGDGATYASKAFLCRAVARLVHEEGMRVDVATGGELQVVL
                     AAGVPAERTVLHGNNKSLDELRLALTAGVGRIVIDSDDELDRIESLVASGLPVPRVLL
                     RINPGIEVHTHEHVRTGNLDSKFGFPLPTGQADDALARARASAAVDLVGLHMHIGSQV
                     FSVENFLEGLRAVAPFVVAADLPELVVGGGLGVAYVEGEAAPTITEWATAVVQECRAA
                     GITAEIGVEPGRAVVGAAAVTLYTVGTVKFIPGVRTYVSVDGGMSDNPRPVLYGSGYE
                     TFLPRAATSRREMPIRLVGKHCESGDLLVREGRVPADLAVGDVIATPVTGAYGHSMGS
                     NYNKVLRPAVVFVADGVDRLVVRRETVEDLLATDVG"
     gene            complement(39347..>40971)
                     /locus_tag="PLZ72_01960"
     CDS             complement(39347..>40971)
                     /locus_tag="PLZ72_01960"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNA35090.1"
                     /translation="ARRYDRRLMRDELTAALRDTLGALGVDAPAEIHLERPARREHGE
                     FSTNVAMATAKAAGRNPRELAQQLADALTADPPRHVTAVEVAGPGFVNFRLDPAWLHE
                     LIGTVVDAGADYGRSDVGAGTRVMVEYVSANPTGPVHAGHARGAVYGDSLARLLRFVG
                     HDVSDEFYVNDRGVQMQTFAASLAARKAGQEPPEDGYRGQYIVDWAAEMPDGADPLEW
                     GEAHALADQRRVLARLGVEFDTWFSERSLLASGAIADTLDDLRERDMVEEREGAVWLR
                     STVFGDDKDRVLVKSDGEYTYLLPDIAYHRDKFARDFDLVINVWGADHHGYVPRMKAA
                     IQALGHDPADLEVVITQLVRLERDGEEVKISKRAGDLITLEDLIDEVGADAVRLTYLL
                     QSVDSPQTVDLGLIVQQSNENPVFYVQMANARIASIARVAAERGIERRPLAEVDLSLL
                     TAERELEVLRQLDVLGEVVLLAARERAPHKVTTWVRELASSVHGFYHDCPILHPDTPD
                     ELRQARWWLAEAAGVGLRVGLGLLGVSAPERM"
BASE COUNT         5897 a        14368 c        14796 g         5810 t
ORIGIN      
        1 cagttcgagc gcgtacggcg cactctccgg attgccggcg tcgacgccgc cggctacggc
       61 ccgacgttcg ccctgctcgc cctcggaccg ctgatcgcgg cgggcctggc gttcgccgtg
      121 cccgagaccg gcgggcgtga gctcgaggag atcaaccagg aggcctgagc cagtctctga
      181 tggcggcgac cacgtcgtcg tcggaccgct tcaggtcgtg ccccgcgccc gccagcgtca
      241 ccgaccggac cgggccgggg atcagcgtct gggcctcggc cagctcctcg ggcgtcccga
      301 acgtgtcgcg gtcgccgctg atgaacagca ccgggacccg cagctgcggc aggtgctcgg
      361 tgcgcaaccg gtccggtcgg cccggtgggt gcagcgggta gctcatcagg accagaccgg
      421 cgacgggcag cgggccctcg ccgtcggcac ccgccgcgac catcgagcag atccgtccgc
      481 ccatggatcg accgccgagg acgaggtgct cgggaccggt cccccaccgc ctgcacagct
      541 cgtcgacctc ggcgcgcacc gccgccatca gcttgggtgc ccggtccggc gcccgtcggc
      601 cctcacggcg gtacgggaag tcgcgacggg ccacacgcag cggtgccacc ccggcctcga
      661 cggcgcgcaa ccccgggtgc tcggcgtcgg agcccgcacc gggccacagg agcatcccct
      721 ccacccctcg gggtcggaac ggtctcgcca ccgctcagcc gagcaggatc cggcgggcct
      781 ccgccaggtc cgcgatccgt tccgccgcga cgtcgtcgtc ggcaccgtgg tcgggatggg
      841 cggcccggag ctggtcgcgg aaggcctgtt ggacgtcccg gcgcgacggc ggtgcgtgac
      901 ggctccggtc ggtgaccagg cgcaggacgc gcagcgccca cacgaccggg tccgccgccg
      961 cgctgaccgg caggtcgcga ccccggccaa ccaggtgcgc gatcagcggt gagccgatcg
     1021 gtccggacca gcgcattccc ttgcggaccg ctgcgaccac cgccggccgc aacggggcgg
     1081 cgacggtccc ggtcgcgtag accgcggcca gcacgtgctg ggccggcgtt ccccgggtgt
     1141 cgtcgatctc caggtggagg gactcacccc gacccacgag tcggtggtga cacggcctca
     1201 acccgatccg gtcggtctgg agtcggtgcc gggcccgggg ctgcgcgacg tgacgcccgc
     1261 gctccacgtc ggacaggagc tgggtgacct cagctgcggt gtcctcgtcg aggtcggggg
     1321 tgaaccgggc cgcgaccgct ccgaggagga tgcccccgaa gccgggagcc gggtcgcacg
     1381 gaaggttcag ctcccccaac gcgagtcgtc gtgtcggggc gatcgggcgg gactggtacg
     1441 cctccagctc ggcgaggatc accgcttcaa cgtacccggc ggggccgcgt cgctacacgt
     1501 aggtgcgcag caggtcgcgg acgtctccgg cgacgacccg cagctcgtcg tcgtcgaggg
     1561 agtcctcggc ccccggggcg gcggccaggg cgtcgcgcag caccacggac cgaccgacca
     1621 ggtgacgcca ctgcggcgcc tcgaagccga acggcgggcc catgcgttcg agggtgccga
     1681 ccacgtcgtc gacggcgagc accgacgcgg cgtcgtcgcg ggaggcgagc ttccccgaag
     1741 ccaggaacag ccggtcgagc agctcgtgga cggcgatccc gtcatcggcc gacacgtcgc
     1801 cgaccagatc ggggtcgtcg gggtcgggca tctcgtcgag gatggcctcg acgtcctcct
     1861 cgtgttcggc gtagacgacc aggtcgccgt tgtggtccca ctcgtagggc agatccgcga
     1921 cggtcagcga gtcggccagc atggtctgca gcgcggcggg ccaggagccg acctcgtaga
     1981 ccaccttggc cgccgcagcg tcgagcgccg gacgggccga cgccatgacg tcgtcgatca
     2041 ggtcgtcgac gtcgtcctcg tcctcggggt gcaccgacag gacggtcccc tgccaggcgt
     2101 gggggatccc gtgggagtcg aggaggctgg cgagcaggcc gcgggactct ccggcccagg
     2161 tggtgcagtc gtagaggagc tcgtcgcctg tcccgtcgtc ggtcgtctcg tcgtcggtca
     2221 tctcgtcgtc ggtcgtctcg tcgtcgcgca tcacggccat gatcgcaccg acccgccccc
     2281 tcccgccccc tcccgccgga ggcccggcgt tggggtgagg tcgggagcct cggtaacgtc
     2341 tggacccatg acgaacaccg cacccgaccg caacctggcc atggagctcg tccgcaccac
     2401 cgaggccgcc gccatggccg ccgccaagtg ggtgggccgc ggcgacaagg agggcgcgga
     2461 cggcgccgcg gtcgacgcca tgcgcctgat cctgggcacc gtcagcatga ccgggaccgt
     2521 catcatcggc gagggcgaga aggacgaggc gcccatgctg ttcaacggcg aggtcgtcgg
     2581 cgacggctcc aacccgcagg tcgacatcgc ggtcgacccg atcgacggga ccacgctgac
     2641 ctccaagggc cgcggcggcg cggtggcggt gatcgccgtg tccgagcagg gcaccatgtt
     2701 cgaccccggc ccgtgcgtct acatggagaa gatcgccgtc ggccccgacg tggccggcca
     2761 cgtcgacatc aacgcgtcgg tgagcgacaa cctgcacgcg gtggcccggg ccaagggcgt
     2821 cgacgtccgc gaggtgaccg ccaccatcct cgaccggccc cgccacgacg acctggtcgc
     2881 cgaggtccgc gagaccggct cgcgcatccg cctgatcggc gacggcgacg tcgccgcggc
     2941 gatcgccacc gcgtaccccg actccggcgt cgacatcctg ttcggcatcg gcggcacccc
     3001 cgagggcgtg atcgcggccg ccgcgctcaa gtgcatgggc ggcgagctga tcggcaagct
     3061 ctggccccgc gacgccggcg agcgcgacgc ggcgatcgcc gccggctacg acctcgacaa
     3121 ggtgctgggc accgacgacc tcgtcgccgg cgacaactgc ttcttctccg ccaccggcat
     3181 caccgacggc aacctgctcc agggcgtcca ctacgaccgc ctcggcgtca ccacccagtc
     3241 gctggtcatg cggtcccgtt cgggcaccgt ccgcctggtc gaggcccgcc accgacccgg
     3301 caagtccgag gcctgaccgg acgccgtcgc cgtggagttc gacgacgacg cccggctcga
     3361 cacctcgcac atcgacgacg cccgcgggcg gggcggcgcc gggcctgatc tcggcggact
     3421 gcccggcggc ttcggcaccg gcgccaagct cggtggcggc ggcctgatcg gcctgctggt
     3481 ggtcatcgtc atcgccttcc tcagcacccg cggcggcggg ttcgcgacca acgacatgct
     3541 cgagccgtcc accacggtcg gcgaccagag cgccaccgag gtggcgcggg agtgccgtac
     3601 cggagccgac gccaacgaac gccaggactg ccggatcgtc gccgtggtca acagcgtgca
     3661 gaggtactgg tccgacacct tcgcccgcag cggcgtcacc taccgtccgg cgcgcaccca
     3721 gctgttcagc aaccgcacct acagcgggtg cggccccgcg tcgtcggcca ccggtccctt
     3781 ctactgcccg gccgacgaga cggtctacgt cgacctgacg ttcttcgacg cgctgtcgca
     3841 gccgccgttc aacgcggcgg gaggtccgtt cgcccaggcg tacgtgatcg gccacgagta
     3901 cggccaccac gtccagaacc tgctcggtcg ctccgccggc gccgaccgcc agggtgcgac
     3961 gtccggctcg gtccgcctcg agctgcaggc ggactgctac gccggggtgt gggccgccaa
     4021 cgcggtgtcg accgggttcg tcaccgagat cacccccgag gacatcgccc agggcctcga
     4081 cgccgccgcc gcggtcggcg acgaccgcat ccaggagtcc gccggtggac gatcgaaccc
     4141 ggactcgtac acccacggga cctcggccca gcgccaacgc tggttccagc ggggatacga
     4201 caccgcggac ccgaccgcct gcgacacctt cagcggccgc atctgagcgt tcatcgaccc
     4261 agcgatcgca cggccacgcc cgccgccggt tcgaacccca ccgcgaccag ggacgggtga
     4321 tccggaccga ccgccacctc ggcaccccgc cgggcggcct cggagcacca ggcgttcacc
     4381 agctgccgtc cgatcccctc gccccggcgg tcctcggcga ctcccacgct caccacctcg
     4441 gccgccgtcg acccgttccc gaccgatccg agcgccgcgc ccaccaccgg tcccggagtc
     4501 cccggatcct cggcgaccac cacgagcggc cggggcccgg gtctgctcgg cggttcggtc
     4561 gggtcggttc cgaggagccc ggcgacctcc ggctcgacca tggagaccgt ccgcagcgca
     4621 agctcgatgc cgccgcgtcc cacgacggcg gggccgccga gcggttcctc gcggatcgcc
     4681 acccagtggg cctgaccgtg tcgggggtga tccggtcctt ggcggcgctg ctcgagcagg
     4741 tgcaccgagg cgaccgacca acgctggatg agcccggcca gggcggtgac ggcggccgcg
     4801 atcagctccg gttccgcgct ctcgcggatg gtggcgtgcg ggtggaacgg ccacacgtcg
     4861 ggacggtcga acggcccgca gcgcaggcgc ccccgcagcg agctcagcgc ctcgggatcc
     4921 gccgcggcga ccgccaggtg cagcgtcggg gtctcgggca ggaacgtggc ggcggggccg
     4981 acttccagtt ccagcggccg tgacgtcgcg gccacccgcc gcaggacgcc gagcacgtcc
     5041 gggacatcgg gctcggccac gttggtcggc gggacgaggg tgacgtgcgg agcgacgtgg
     5101 aacggctcga ccaacccgac ggcacgtcgg agggcgtcga tctccgcggc ctcggcctga
     5161 ggggcgagga ggacgacggc gagacgacgg cgggccaccc gccggacgct agttctcctg
     5221 attcccgtga ccgttcgcgc caccagtggc gccaggagtc atggaaacgg gactgatcag
     5281 gcagcctcgg cggcggtgag gcggacctcg gcgcgcacca gggcggcctt ggcctcggga
     5341 tcctcggagt cggcccgcag ggcctcctcg gcgcgctcct tggcggtccg ggcccggtcg
     5401 acgtcgatgt cggcagccat ctcggccacg tcggagagga tggtcacccg ggtgttgccc
     5461 tgctcgtcgg ggggtgacac ctcgacgaag ccctggtgca cggcgatgac cttctcggag
     5521 ccgtcctcga gctgcagccg caccgggtgc gtggcgagga cgccgatgaa ggggatgtgc
     5581 ccgggcagga aggcgatgtc gccggtggtg gtccggcaga tgaccatccg ggcctcgccc
     5641 gagtaggcga tgcgctccgg ggagacgagc tcgacctcga gggccatcag gcgttcgcct
     5701 ccagctcctt ggccttgcgc atggcgtcct cggccccgcc gacgttgaag aacgcctgct
     5761 cgggcaggtg gtcgaggtcg ccgttgacga gcatctcgaa gctctcgacg gtgtcctcga
     5821 ccggggtgaa gatgccgggc aggccggtga agacctcggc cacgaacatg ggctgggcca
     5881 ggaacttctg gatcttgcgg gcccggtcga cggtgacccg gtcctcctcg gagagctcgt
     5941 cgatgccgag gatggcgatg atgtcctgca gctccttgta tcgctgcagc atctcctgca
     6001 ccctgcgggc cacggcgtag tggcgctcgc cgacgacctc gggggtcagg atcgagctgg
     6061 aggacgccag cgggtccacc gccgggtaga tgccgagcga ggcgatgtca cggctcagct
     6121 cggtggtgcc gtcgaagtgg gtgaaggcgg tgaacggcgc cgggtcggtg tagtcgtccg
     6181 cgggcacgta cacggcctgc agcgacgtga tcgaacggcc accggtcgag gtgatccgct
     6241 cctgcagctc gcccatctca tcggcgaggg tcggctggta gcccaccgcc gcgggcatgc
     6301 ggccgagcag cgtcgacacc tcggagccgg cctgggtgaa ccggaagatg ttgtcgatga
     6361 acagcagcac gtcctggccc tgcacgtcgc ggaagtactc ggccatggtc agcgccgaga
     6421 gggcgacccg gaggcgcacg cccggcggct cgtccatctg gccgaacacc agggcggcct
     6481 tgtcgagcac cgaaccggac tcgcccaggg cggtctcacc catctcgatc agcaggtcgg
     6541 tgccctcgcg ggtccgctcg cccaccccgg cgaacaccga cacgccgccg aactgcgagg
     6601 cgacccggtt gatcatctcg gtgatgagca cggtcttgcc cacgccggcg ccgccgaaca
     6661 ggccgatctt gccgccctgg aggtacgggg tgaggaggtc gatcaccttg atgccggtgg
     6721 cgaacatctg cgccttgggc tcgagggtgt cgaaggccgg ggcctctcgg tggatggccc
     6781 agcgctcggg cgagtcgacg gactcgacgt cgaggggctg gcccagcacg ttgaacacgt
     6841 ggccgagggt ggcgtcgccc accggcaccg agatgccgtg gccgaggttg cgcaccgcgg
     6901 tgccgcggac caggccgtcg gtcggcttca tggcgatggc ccggacccgg ttgtcgccga
     6961 tctgctgggc gacctcggcg accaccacgg tgtcgacgcc gtcgacggtg atggtgaact
     7021 cgagcgcctg gttgatctcg ggcacggagt ccgacgggaa ctccacgtcg accacggggc
     7081 cggcgatggc gacgatgcgg ccgtcggtca gttcggtgtc ggtgacggtc atggtgactc
     7141 ctgggctgcg gtctggtgac ggggtggtcg gtctacgaag ggtttcggtc gggatcggcg
     7201 tcgatcgggg tcaggtccgg accggacccg actggtgacg gtggtcgagc ggcaggaacg
     7261 gctcggtgtc gaggtggtgc aggagcaact cgcctggatc ctcgcggtcg tccgcgaggg
     7321 cctcggcgcc gccgatgatc tccatgatct cggtggtgat ggcgtcctga cgcacctggt
     7381 tcatcttgcg ggtgtacttg acgatgagct cttcggcgtt gtcggtggcg gacttcatgg
     7441 cccgctggcg gttggccagc tccgacgcgg cggcgtcgag cagcgcggcg aagagccggg
     7501 actcgacgta gcgcggcagc agggtctgga ggaccgcagc ggccgacggc tcgaactcga
     7561 agcccgcggt ggccgaggcg tcaccgcctt cggcgatggt gtcggcatcc tccaggggca
     7621 ggaagcggcg aaccaccacc tcctgggagc cgagggagac gaaccgggtg tacacgaggt
     7681 cgatgcggtc gcagccgccg ttctcgtaga ggtcacgcac ccgagcggcg acggcgacgg
     7741 cgtcctcgaa gctcgggttc tccgagaagc cctcgaagct cgcctcgatc gggtagtgac
     7801 ggaagctgaa gtacgccttg ggcttgcgac cgacggtcac cagggcgtac tccttgccct
     7861 cggaccgggc ggcctggacc tctcgctcag cggccttgat gaccgacgag ttgtaggcgc
     7921 cggccagacc tcggtccgcg gccaacacga cgacgccgac cttctcgacc acgcccagcg
     7981 ggcgcaggag cgggtggtcg acctcggcgc cggccgcgga caggtcctgg atcacctggg
     8041 tgatgtgctc ggcgtacgga cgggcctcgt tggcccgctg ctgcgccttc accacgcggg
     8101 tggccgcgat gagctccatg gcccgcgtga tcttcttggt gttctggacg ctggcgatcc
     8161 tccgtcggag gacccgctcc tgtgcaccgg ccatgtcggc tccctacgtc ccgtccgcct
     8221 cagaccgtgg ccgcgtcgtc gtcaccgtcg cgggtgacgt cctcttcggg gaggtgacgg
     8281 ctcgaggtgg cgatggtgga ttcggcgtcg gcctgggcct gagcggccgg ggccgaggag
     8341 tcgacgctgg tgccctggaa ctgcgcggcg aacgccttga ccgcggcgtc gaaggcgtcc
     8401 tcgtcgtcga tcttgccggt gctgacgatc gccgagagca tcccggcgtg gcgggtgcgg
     8461 aaccagtcga gcaggccgtc ctcgaacgag cggacgtcct cgacggcgat ggcgtcgagg
     8521 taaccgcggg tgccggcgaa gatcaccacg cactggtcct gcaccgacat cgggctgttg
     8581 atgccctgct tgagcagctc ggtcaggcgg tagccccgct cgagctgcgc cttggacacg
     8641 gcgtcgaggt cggagccgaa cgccgcgaac gcctcgagct cgcggaactg ctgcaggtcc
     8701 gacttgagcg tgcccaccgc ggtcttcatg gccttgatct gagcggcgga acccacccgg
     8761 gacaccgaga tgcccacgtc gaccgccgga cggacgccgg acttgaacag gtcgtcctgg
     8821 aggaagatct gaccgtcggt gatcgagatc acgttggtcg ggatgtaggc cgacacgtcg
     8881 ccggccttgg tctcgatgac cggcagggcg gtcagcgagc cgccgccgcg ctcgtcggac
     8941 agcttggcgg cccgctcgag cagacggctg tgcaggtaga agacgtcgcc ggggtaggcc
     9001 tcgcggcccg gcgggcgacg cagcagcagc gacacctggc ggtacgcctc ggcctgcttg
     9061 gagaggtcgt cgtagaccac cagcgcgtgc tcgccgttct ccatccagtg ctggcccatg
     9121 gcgcagccgg cgtagggggc gaggtacttg aacggcgcgg ggtcagacgc cggggcgacg
     9181 acgaccacgg tgtactccat ggcaccgcgc tcggcgaggg tggcgacggt ctgcgcgacg
     9241 gtggaggcct tctggccgat cgccacgtag atgcacttca cgcccagacc ctgctggttg
     9301 aggatcgtgt cgatggcgat cgtggtcttg ccggtcttgc ggtcgccgat gatcagctcg
     9361 cgctgacccc gaccgatcgg gatgagcgag tcgatggcct tgatgccggt ctgcatcggc
     9421 tcgcccacgg gcttacgacc catgatgccc ggggcctgga tctccatccg gcggtcctgg
     9481 acgttggtca gcgggccctt gccgtcgatc ggctcgccga gggtgttgac gacgcggccg
     9541 agcacgccgt cgccgacggg gaccgagagg atgttgccgg tcgccttgac cgtctggccc
     9601 tcctcgatcg agtcgacctc gccgagcacc acggcaccga tcgagtcctc gtccaggttg
     9661 agcgccaggc cgaacaggcc cgactcgaac tcgagcagct cgttgaccgc cgcgtcgggc
     9721 agtcccgcca cccgggcgat gccgtcgccg acctcgatga cccggccgac ctgggtcagc
     9781 tcgacgtcgg gggcgaagcc ctccaggttc ttcttgatcg ccgcggtgat gtcggcggtg
     9841 ttgatcgtga gctcagccat ctcagaaggc ctctcggagt tggttcaggc gggtacggac
     9901 ggagccgtcg aggacggagt cgccgatctg ggtgacgacg ccacccatca cggtcgggtc
     9961 gacgacgttg cggatggtga cgtcgatgtt cgtgcgggcc ttgagggccc gggcgagatc
    10021 ggcgagctgg gcgtcggtga gcggcaccgc ggagcgcacc tcggcgacgg cctcgccccg
    10081 ggatgcggcg gatcgctgcg ccatcgcttc tgcgatggcg gccagatcgc cgatgcgtcc
    10141 ggcggagacc agcatcgaga cgaacgacac ggtcgtccgc gtggccttgc cgtcgagaag
    10201 gtcctcgacg atctgggccc gtcgctccac cggcaggtgc gcgtcggtca gggtgccgcg
    10261 cagctcgtcg ttggtctgca ggacccgtcc gaggcggaac agctcgtcct ccacctcgcc
    10321 gatgttgccc tcggccgcgg cgatgccgta ggcggcatct gcgtaggcgg agatctgtgc
    10381 gtcgctcatg ttcgtcgtcc cgttgcgaag gttggtctgc ggtgccggcc ggtcagttct
    10441 gtgcgccgac ctggctgatg tagtcgtcga tcagtcgctg ctgggtggcg tcgtccagcg
    10501 aggaggccac gacctcctcg gccgcgccga gtgcgagccg ggagagctcc ccggacaggt
    10561 ccgcggtggc ctgcatgcgg gtggcggcca ggtcggcacc cgcccgctcg cgcagggcgg
    10621 cgatctcggt ctcggtgcga gccgcggtgt cgatgccgag ctggtcggcg gcgcgacggg
    10681 catcctcgac gatgcgggcg gcctcggagt cggagtccgc gagcgccgcc ttgatccggt
    10741 cacgctccgc ctcggcggcc tcgcgggccg ccgcggcctc gccgaggtgc tcggcgatcc
    10801 ggtccgagcg agccttgaga ccgttgacga tcggggcctt gccgaacttg accagcaggg
    10861 tggcgacgat caggaacgcc agcgagcccc agatgacctc gttgatgtcg gccggcagcc
    10921 acttgccgtt ggatccctcc gcggccagga gcgccgggga gacgatggag tgcagcaggt
    10981 tcatgtctgg ttcctccgga ctcagcgcgg tccgccggag cggttcacgt agtcgtccac
    11041 ggtcgatcgg tgcgaggccg cgtcgagcgg cgcctggacc accttcgagg ccgcagccac
    11101 cgccaggtcg gcgacgtcgc cgctgagctg gcccaacgcg gcggcccgct cggcgtcgag
    11161 ctcggccatg gcggcctggc gctcggcggc gatctcggtc tccacgccgg cgaccttctg
    11221 tccgcgggtc gcctcggcgg cggcgcgggc ctcctcgagc acgcgggacg cctcgccccg
    11281 cgcctcggcg agcgtggcgt cgtagtcgcg acgcacctgc tcggcctggg tcgaggccgc
    11341 ctcggcggcc tcgaggtcgg cctggacctg agcgtcacgc tgggcccgga cccgcagcag
    11401 cgggggcagg cacacgtagc gcatcaggat ccacaggagg aagaagaaga cggcgcccca
    11461 gaagatctcg ccgacgacgt cggggacgac ggggttgatc ttctcggcgc tctccgcggc
    11521 ctccgcggcg agcacactca cttgagcgag catcgggtac tccggttcgt ggttctacgg
    11581 gttcttcggt ggtcaggttc cggaccgggg tccggatgag gtccgcgtca gacgaacttc
    11641 aggaggatga agaccacgaa gccgatgagg gcgagggcct cggtgaaggc gatgccgagg
    11701 aacatcgtgg tctggacctg accggcggac tcgggctgac gggccatggc ctgcacggcc
    11761 tggccgacga ggtagccgat tccgatgccc gggccgatgg cggcgaggcc gtaggccatg
    11821 ccggcgcccg aggcggcagc ggtcgccttg gcggcgtcgg cgtcgagacc ggcggcgtcg
    11881 ccgacctggg cgatgatccc tgcggcgtgg tgcagtgcgc tcattggatg ggttctcctg
    11941 gtgtgcggtg gtctggcgac cggtttgctg attggacgat ctgttcgggt gccgccggtt
    12001 cgggcgacgg gctcagtggg ccgggtgcag ggctccaccg atgtacacgg cggtcagcag
    12061 gctgaagatg aacgcctgca ggaacgccac catgaactcg aagccggtga aggcgacgag
    12121 catggggaag ctgagcacca ggacgccggc gaggacgctg gcggaccaga gggtgatgca
    12181 cagcacggcg aaggtcacga gcaggatgtg acccgcgagc atgttggcga agagacggac
    12241 ggccagggag aagggccgga tgatgaaggt cgacagcagc tcgatcggcg tcaccaggat
    12301 gtagagcgcc ttgggcactc ccggcgggaa cagggccgac ttgaagtagc cgaggccgtt
    12361 gtgcttgacg ccgacgccga tgaacatcgc ccaggcgacg agggccagca ccagcgggtt
    12421 ggccatgcgg gcgttggcgg gcatgtgcgc cgaggggatg acctcgaaga tgttgccgat
    12481 ccagatgaag aagaacatcg agatgagcat gggcaggtag cgcatgccct cggggccgat
    12541 cgccggcagg accacctgct tctcgacgaa gtcgatcgag acctccatca cgttctgcag
    12601 gccggagggc accagcttgg acttgcgtcc ggcgatgagg aacaggatca ccggcacgag
    12661 catcgcgatg aacgagatga aggtgatctt gttgaactcg tagaacgtgc cctcgccgaa
    12721 ccacgccggc cacttgacga tgtggtcgat cggtgggaag tgcatgcctc ccccaccgcc
    12781 gtcttcggcg gcgaggagga tcgacggggc gttgagcagc gcgaggccgg gcattcggtc
    12841 gggctccttc tacggcgcgg tcggtgactg cgctgaggtg gacgttgacg ggcccgacgc
    12901 gtcgtcggcg acgggcagat acgggttggg ggtcgcctgg ggcttgaggc cggggaaggc
    12961 cagcgagctc gagatgtggc ggagctccca gaacagcagc cccaggtggg tgatgatgat
    13021 ggtcagcccg agcggtacga gctccaccca gggctggtca cggaccgcga gcacggcgac
    13081 catgatgagg ccgaggcgca gcaggaagcc gaacatggcc gcggcgccca tggccgccgc
    13141 tccgatgcga ccgcccacct gcagcagcca gccggccaga aggaagttca cgatcacgat
    13201 cgccaagccg tagagggtgg agaacacgcc gtcgagcccc cagaacgcgg ccgaggcgat
    13261 gaccgcgatc gggatcgccc agacgctgcg acgcacgatg tcgaacgcga ccgccatggc
    13321 cggcgacgga ccctcgacgc ggggcccgag catgccgtcg gcggggacct tgccctcgac
    13381 gctcaccggt ggccctcggg ggattcgacc ggacgcggct cggatcggtt ggcggcgtgg
    13441 tactgggcgg cggggcggtc gacggggatg cggccctgtt cgaccagctc gtcgagctgg
    13501 cggtggtacg agaagtacat cgcgagcccg gcaccgacgg caccgaagac ggccaggccg
    13561 acggtgaaga cgggcgccgt gccgagggtc cggtccagcc agagcccgat caacgccatg
    13621 atcaccggcg cgaacaccag ctcgaagctg ccggcgctgc ggtgcaacga gcgcgtcagc
    13681 tcggtctggg aaccgtcgcc gggctcggtg gaggtgcggt ggtcgggcat ggggtgcgtc
    13741 ggttccaggt gcttgtgaaa cctttcgcaa tctacgtgga ggccgtgacc ccctgcaaat
    13801 ccgagtgggc tcgtcgcgac gccggccggc agccccggat cagggatggg acgtgggtgc
    13861 ggtgccggtg aaggtcgggg gcacgaccgc gggcgcgggg gccgcggtcg gagcggcggc
    13921 acgttcgatc gcctcgacga tgcgatcggc gttcgcccgc tgctcgtagg acgagcggat
    13981 cagccagaac cgcaggtagc gggccagcga gtaccgcacg aacagcgctc cccccgcgat
    14041 gaccagcgcg atgcccagga acgatccctg ggccatgtag gcgcgctgct cggcggggaa
    14101 gtcgccctgc agcccgacct ggatggcccc gaacagcgcc agcacgaggc cgaccaccat
    14161 caggacgacc ccgagggcca gcaggcgctg ctccccctcg gcggatcccc ccttgagctt
    14221 cagggagtcg atctcctcgc ggaactgcgc cacgcgggcc gcgtcggcgc cgggcacggt
    14281 gctctccggg ttgctcattg ttctggtccg ctccttgtcg atctgcgttc gggcgatctg
    14341 ttctctgggc tgtcgttctc ttggctgtcg gtgggttcag ctcgtccggt ccccggacgg
    14401 attctcgcgg agatcaccgg tcatccgccg agataggcgg ccgacagctc gcgttcgacc
    14461 tcgtcgggtc ggccgacctg gcggatcgtg ccgttgacca tgatggccgc cacgtccgcg
    14521 acgccgagga cggtgcgggc gaactgctcg acgaccagga tggaggtccc ggaccgggcg
    14581 atctccgaca cgatgccgta gagctcctcg accaccagcg gcgccagacc catcgacagc
    14641 tcgtcgagga gcagcaccgc gggttcgacc gcgagcccgc gggccatggc cagcatctgc
    14701 tgctcgccgc ccgacagcgt gccggccacc tgggtccgcc gctccttcag ccgtgggaac
    14761 tgcgcgtacg cgacctcttc gacccggctc agcgacaccc cggcgtaggt ggccatcacc
    14821 aggttctcgc gcacggtgag gttggggaag atccccttgc cctcggggat ggtggtcagg
    14881 ccccgtcggg ccaggtcctc gggactgaca ccggtgacgt cgcggcccgc caccatcagc
    14941 tgcccgccgg tcggagcgag ctgccccgac gcgaccgcga gggtggtggt cttgcccgcc
    15001 ccgttggggc cgagcagcgc catgacctgg cccggcatca cggccaggtc gatcccgtgc
    15061 agcacttcga tggtgccgta ggcggcccgc acgccgcgca gctcgatcag cggttcgctc
    15121 atgaggcccc tccccgaccc gggccgtgac cgttggcgtg gccggctgcc tcggcgatcg
    15181 ccgcagcgcc gaccagttcg tcgtcgccgg ccggtgcgcc caggtaggcg gcgaggaccg
    15241 cctcgttgga gcggatcgcg acggggtcgc ccgccgcgat cacctggccg aagtcgagca
    15301 ccgacaggtg ctcgcacacc cgcatgacca gcgagatgtc gtgttcgacc agcaggatgc
    15361 ccagtccggc gcccgcgagg tcgaccagca ggttcccgaa gtcgaccgtc tcacgctcgt
    15421 cgaggcccga ggcgggttcg tcgagcagca gcacccgcgg acgggcggcc agcgctcggc
    15481 ccagctcgac caaccgggcc tgaccggtcg gcagggaccc cacgggcagg tcggccagct
    15541 cggtgagctg caggcgctgc aggatcagct cgacctcgtc gaccggggag atgtcggcgc
    15601 ctccggccag gaaggccggg gccggggccc gccgccgcga ccagccctta cggatctcca
    15661 gcccgacacg gacgttgtcg cgcacggtga gcgacgagaa cacctcgagg cgctggaacg
    15721 tccgaccgat cccgacccgg gcccgctggt gggtgctcag cttcgacacg tcgcggccgt
    15781 cgaggacgac cctgcccgac gtcggggtga tcaccccgca gatcgcgttg aacgtggtcg
    15841 tcttgccggc accgttcggg ccgatgagcc cgcagatccg cccgccgtcg acccgcagcg
    15901 acacaccacc gaccgccagg tgacctccga agcggaccga caggtcggtg acctcgagcg
    15961 agctcacggc gcacctccga tcccggctcc gacggcgctc ggcatcagtt cctcgtccag
    16021 gccgagcgag cgaccggcgt cgagggcgtc ggccctgctc agcggtcggt cgaggcccag
    16081 ctcgtcgggc gacgggtgct cgaccttctt cagcgattcc ggcaccaccc ccatgacgcc
    16141 gatggccacc cgcagcacca ggaacgtgaa caccccgatg cccaggatcc gccacccgtt
    16201 ggaggagatc cgatcggccc agggcaggaa cgcggcgacc agcaggccga gcgccagcca
    16261 gatgccggtg gcggcccgtc ggggtcgggg gccctcgggg gtgtcgggtg cgaagaggat
    16321 gggcagcaac ggcaccaccg cgacgacgaa gaccagcaac gccgcgaaga acgaccactt
    16381 ggtgatcacc ccggcccgcg tggcgaacca caggccgacg ccgccgacgc cggcgatgac
    16441 cagcgacggc accgactcac cgacggcccg gtacccggcg gccatctgag gcgcggcacc
    16501 atcgggctcc tggcccaggc tgattcccat gaggcccggt gtgaagctca gcacgtcagt
    16561 caccggcatc gacacgaagc ggaagatgcc gatcgcgttg gccgcgaaga tcgtcgatgc
    16621 gatcgggatg gccccgagca ggagaccccc caggaacgcc ccggcgaccg acccgatgcc
    16681 gccgaccacc gccagcatgg tgatgggcag cgagttggtc agcccgaagt cgtccgcgat
    16741 gaacgtgcgg ccggccagcg ctccaccgag gccggcgatg gcggccgaca gggcgaacac
    16801 cccgaccttg gtcgacgtga ggttcagtcc gagggtcgcg cacgccaccg gcgagtcctt
    16861 catggcggcc acgcgccgcc cccaggcgct gcggcgaagc agcaccagca ggttgccgag
    16921 gagcgcgaag gtcaccgcga acaggatcag ctggcggtag tcgttggtga cctcgaagcc
    16981 gccgatgctc agccccggca cctggatcga cccgccgggc atcacctcgg actggccgaa
    17041 gaccatcgag gtgcagaaca gggcgaacgc ggcggtggcg agcgccaggt agatgcccga
    17101 cagccgcagc gccggcaggg cgacgaccgc gccgaccacc gcgcagagca ccacggcgat
    17161 cgctacaccg accggtgacc cggtggcccc ccaggtggcc atggcgagcg ccccgatgcc
    17221 ggtgaaggtc atctgggcga gcgagatctg accggcgtag ccggtcagag gcaccagcga
    17281 cagggtgatc agcgcgaagc cgaagccctt ggagaccagg ttgaggtcgg gcgccgacag
    17341 cagggagccg accgccgcgg cgacgagcac cagggcgatc ccaccccaga tcgccgtgac
    17401 ctgcgacggg ggccgcaccg gggatcgggg tcggctgacg ccgtgggccc gcagacgtgc
    17461 ctcgggctgc acgaggatca ccacgaatag catcaccgcg gggatggcgg tgcgcaggtt
    17521 ctggaggctg accgggccca gcgcgtagga cgggtcgatg tagccggtgg cgtaggactc
    17581 ggccagaccg aggaccaccg caccgaggaa cgcccccggc agcgatcgca gccggccgac
    17641 caccgccacg gcgtaggcgt tgacgaccag gagcgtgagg gtgacggcgc tgagggtctg
    17701 ctccgaggcg atcagcaccc cggacaccgc ggccaggccc gctcccagcg cccaggccag
    17761 catggcggtg cggcccggcc ggccgccgtt gagctgcagc agcgcccggt cgtcgacgat
    17821 ggctcgcatg gccacgccca gccgggtgcg ccgcagcacc aggcggagtc cgaccgccac
    17881 cgcgatggcg atcgccatgg tcaggatccg cgagtaggga accgcgactc cggcgatctc
    17941 cacggtctta ccggcgaaga acggcggcac cggacgggcc acgtcgggag gccagatcac
    18001 gttggcgatg ccgagcaggg ccaccatgag gctgaccgtc accacgatct tgaccacctc
    18061 ggacgcgccc tcgatgccgc gcatgatccc gcgctcgatc acggcaccga acaacggggc
    18121 catgacgaac acgaccagga tcaccgacgg gatcaccggc acgccccagc cctggttcac
    18181 ctgccagtag gcgaaggcgg cgagcatgcc gaacgcgccg tgggcgaagt tgaagatgcc
    18241 ggaggtcgtg taggtgacga ccaggcccga cgccgcgatc gcatagatcg cggccgtcga
    18301 caggccggcg atcgtgaagg tcaggaactt gtccaactca gctccgggta cggctcagcg
    18361 cttgcggtcg ggatcgatcg cgcccttggc gggcagctcg gcggtgaccg tggcgatgct
    18421 gtcgggcggg cagtggaagc cgtcctggtc gtcgtcctcg ccaccgatct cgggccacag
    18481 gcgctcgaag ccgtcgccct tgaccaccat cagcatcccg cactccggcg gctcgtcgcc
    18541 gcccgggtcg gtgggcacgt gggtgccccc cgcggtccag tccttctgct cggcagcccg
    18601 atccagcacg cagtcacggc tgatgacgcc cttgttctgg tcaccgcagt ccttggccgc
    18661 ggtcgcgaac agcagccatg ccgaggtcga ctgcagtccg agggccgcct ccttgccgtc
    18721 cggcacgtac ttcttcaggt tgtcctggta ctgcttgatc gcgggccact tgtcggcctc
    18781 ctcgaagggg tggaagccga ggcggaccac cgaaccgtcg accgcagcgg gcccctggga
    18841 gaagtaggtc ctgtcgtaga cgttggtctc gttgagggcg acgcccttcc agccctgctc
    18901 cttgagcttg gacatgaggt tggcggcgtt gccgggctca ccgatccagt agagggagcc
    18961 ggcaccgctg tcgatcacct tctgggcgag cggcgtccag tcggccatgc ccgccacggg
    19021 gtagctgagg gcctcgagct gctggatgcc accgacagcc tgcaccgcgg cgtcgtactg
    19081 gagcttgacg accttgagcg acgggagctc gccgtagacg accacgttct tggccgactg
    19141 ctccgggtag agcttcttga agtccatgat ccactgctcg gacttcttgt tcggcgggtt
    19201 cgggatcggc tggacctggc cgttggacag gctcttctgc accgacaccg cgaaggccgg
    19261 gacgtcgatc agtccgcact tgtggaagtc cgagccctcc ttgccggtga aggacaggtc
    19321 gtcctgggtg aaggccccgc cgacgaagcc gaacaccgag tggcacgcct tggccatggc
    19381 ggcctcgacc tcgaacagct tgccgtcgag gtcgacgggc tcgatctgca ggccgccgat
    19441 cccgccctgc tcgttgcacc agtcggtgaa ggccaccgac gcgtcccaca gctccttgtt
    19501 gaggccgggt cggatggcgg cgccccggtc gttggggacg ccgatgtaga gcttgtcggt
    19561 cccggtgccg ccctcgccct cggcgacctt gaagctgccc ttgccgcacg gcgacggcag
    19621 cgtgccccac tcgctgccgg tcgaggatcc ggccgcggtc gtggtggaac cctccttgtc
    19681 ggatcccttc tcgtcgcccc ccgacgcccc gcaggcgccg agtaccagcg ccatcgtggc
    19741 caagagggcc acgccgacgt gacggatgtg ggtgctccga gccatgtgca gtccccctgg
    19801 gttcgacgtg tggcgttggt cggcgtggtc gcagccggtc cggatcccgg tggaccacgg
    19861 cccgtcccgc cgcgggtcgg ccgacttgcg cggaggttct atcagatgac atcggtcacc
    19921 tgctgccacg agcgcaccgc gctccgctcc cgacgcgccc cccggacgcc ccgtcccgac
    19981 acgcattgcc gtgccccaac cgctctgcaa cgatgggcac ccagaaatct gacaacccgt
    20041 cagattccgg cagggagatc acgcgtggac ctcgcgatcc gcaacgtcac catcgtcgac
    20101 ggcaccggag ccccggcccg ggtcggcgac gtcggcatct cgaacggccg catcgtcacg
    20161 gtcgccgaga cggccgaccc ggccctggac ggcgcggagc gcaccgtcga cgggaccggg
    20221 ctgatgctct gtcccggctt tgtcgatccc cacacccact acgacgccca gctgctgtgg
    20281 gacccgaccg cgtcgccgtc gagcgtgcac ggggtcacca cggtgatcgc cggcaactgc
    20341 gggttcacgc tcgccccgct caaccccggt gacggggact acgtccgtcg gatgatggcc
    20401 aaggtcgagg gcatgccgct ggccgcgctc gaggagggca tcgactggga ctgggagacc
    20461 ttcgccgact acctggcccg cttcgacggg aacctcgcgg tcaacgccgg gttcctggtc
    20521 ggccactgcg ccctgcgccg caaggtcatg ggcaccgacg cggtcgggtc ccagccgacc
    20581 gacgagcagc tgcgcgacat ggaggagctg ctggccgaga gcatccgggc cggcgggctc
    20641 gggttctcga ccacgcgggc ccgcacgcac tccgacggcg acggggagcc ggtggcgtcg
    20701 cgatgggccg aggagcccga gctgctcgcc ctcgccgagg tcgtctccca acaccccggc
    20761 accacgctgg agttcgcctc cgacggctgc ctggacggct tcgacgacga cgaggtccag
    20821 ttcatgatcc ggttctcgag ggccggcaac cgcccgctga actggaacgt cctcaccgtc
    20881 gactcccacg ccccggagcg ctaccgcaac cagctcgccg ccatggacac cgcggcggct
    20941 gccggcgccc gggtggtggc gctgaccatg ccggtcatcg tcggcatgaa catgagcttc
    21001 ctgacctact gcgcgctcaa catgatgccg gactggggcg acatcctggc cctgccggtc
    21061 cccgagcgca tcgagaagct ctccgacccg ggcacgcgcc ggttcatgga ggagcgcgcc
    21121 gcgtcgcccg acgccggcgt gttcgcccgc ctcaccggct ggagcaccta cgtgatcggc
    21181 gacaccttct cggaggccaa cgagggtctg cggggacgcc gggtgggcga catcgcggcc
    21241 gaacgcggca ccggggcgtt cgacacgctc ctcgacatag tcatcgcgga cgacctgcgc
    21301 acggtcctgt ggcccggagc caccgacgcg gaccccgagt cgtgggagct gcgtcgccag
    21361 gcgtggcagc acccgtcggt catgctcggc ggctccgacg ccggagcgca cctcgaccgg
    21421 atgcagggcg ccaactaccc gacccgcttc ttggccgact gcctgcgcgg ccgcaagctg
    21481 accaccgtcg aggacgccgt gcgcatgatg accaccgtgc ccgccgagct gttcggcctg
    21541 agggaccggg gcgtcatccg cgagggagcc cacgcggacc tggtcctgtt cgaccccgac
    21601 gtcgtcgact cggagatgct ctcgatggtc gacgacctgc ccggcggcac cgaacgcctc
    21661 ttcgcgggct cggtcggggt gcgatccgtg ctggtgtcgg gggtcgaggt ggtccgcgac
    21721 ggtcggccga ccgaggcgcg gcccggcacg atcatccgct ccggcgtcga caccgacacc
    21781 gtcaccgcat cctgagcccg ctcggctgcg ggcgccagat caggaccggg cgcgcatctc
    21841 gcccaggcgg ggggcgaacg cggtgaacag cagcagcccc agggccagca gtccgatcgg
    21901 gacgatcccg ttgccacggt cggtgtagac gggcacgagc acgaacgcgc tgagcagagc
    21961 ggtccacgcc cacaggatcg ccacggcgcg acggtgcccg tggccgaggt ccatcagccg
    22021 gtggtgcagg tgcgccttgt ccgcggtggc caccccggag cgccgactgg cccgacggat
    22081 gacggcgaag gccgtgtcga gcaacggaac gccgaggatc accaacggga tcaccagcgg
    22141 agcgaagaag aaccacgact ggcccgagaa cggatcgtcg gactgaccgc cgacggcgat
    22201 cgtcgacgac gcgagcagca gccccagcag caacgccccg ctgtcgccca tgaagatgct
    22261 cgccgggttg aagttgaacg gcaggaagcc caggcacacc ccggcagtga cgatcgcgat
    22321 cagcagaccg acgttggtga cgtcgacgac gccgacctgg aacagctgcc agccgtagag
    22381 gaagaacgcg aacgacgcga tggcgacgat gccggcggcg aggccgtcga gcccgtcgat
    22441 caggttgacc gcgttggcga ggcccaccac ccacagcacc gacaccagcg ccgacaggtc
    22501 cgggggcagg acggtgaagc cgaggaacgg gatgcgcagg tagatcacgg tgaggccgac
    22561 cagggtgagc accgagcccg acagcaccat cccggcgagc ttggccggcg gcgacacgtc
    22621 ccggaggtcg tcgacgagcc cggtcacgaa catgaccgcg gcggccagga ggacgccgag
    22681 gacgttggag ggagcctcga acaccggccg cagccccggc agcagcgcgg cggtcgcgat
    22741 gcccaccacc agacccacga acagggcggc gcccccgagc agcggggtgg gttccgcgtg
    22801 cacgctgcgg tccgacggct cggccatcgc cccgacccga cgcgacagcg accgcaacgg
    22861 gggcaccgac gccgccgtgg cggcgaccga caccgccatc acgatcaggt agtcgcgcac
    22921 cggggcgaca ctagtgccct gcccgtgtcg ccccgaggtg cccggatccg cccgtcaccg
    22981 ccttggatgg acctcgtgaa gagggcgtca tcgtgtcgct ccgatgacac ctcggtgacg
    23041 gttcggcgac gtcgacatca tcgctgtgca ccctcgtgac ggtgtggtac aacggccggg
    23101 ataccaaggg tgatgctggg agctgggagc tgggagcaag agggtggacg gaccggacgg
    23161 tccatccacc tttgcgctgc ggcgtcctgc tgtcgaggag ggacggatgc agtcggagtg
    23221 gggccacgcc acgcgtgcgc aggatctgag acggaggatc ggctcggccg acgtcttcgc
    23281 ccgcggcgcc gtgtcgatgg acgccccctc cgaggtctcc gtacccgagc tgaccatcgt
    23341 cggcgacccg ctcccgcgga cccgacgggt gctcggccgg gtgcccttcc cggtccaggt
    23401 caagacggct ctggtcgcgg ccgacctcgt cgccacgatc tgtgggatgg ccatggccgc
    23461 cggcctgagc gcccgggtcg gcgtcacccg gggcgagacg ggtgcgacgg tgctgaccgc
    23521 actgctgtcg gtgccgatct ggcccctgct gtacgcccac cagggcctct accaggcccg
    23581 gcgcatcacc cgacgggccg acgagttccg tggcatcgtc aactcggtgc tcgccggggt
    23641 cctggtcctc gccggcatct cggtgatcct ccagcaggag cacctgcggg agtggctcct
    23701 gatcacggcg gcgtgcgtga ccggtaccgt cacactcgag cgcgagctcg cccgccgcgt
    23761 catcggcgag ttccgctccc gcggcgtcat gacccgacgg gtcgtgatcg tcgggcggaa
    23821 cgaggaggcg accgagctgg ccgaggcact gcacgccaac cgggggctcg gctacgaggt
    23881 cgtcgggttc gtcgccgatg tccccgtcgg cccggtgatc gacctgcgct cggatcccga
    23941 gctcggcccc tacctcggcc gcactgccga ggtgctcgac ctggtccgtg ccacccgggc
    24001 cggcggggtc atcgtcgcca ccaccggagt cgaccagcgg tccgccaacc ggctcatccg
    24061 cgacctcacc cgcgaggggc tcttcgtcga gctcacctcg tccatgcgcg acatcgccgc
    24121 caaccggatc tcggtccgtc cgctcggtcc gtacccggtc atgtgcgtcg aaccggtcgc
    24181 ccagttcagc tggcgtcgag ccgccaagcg caccttcgac ctgatcgtgg ccagcgtggc
    24241 gatcgtcatc accgcaccga tcctggcggc cgcggcgatc gccatccgcg tgacgtcggg
    24301 tccgggcgtc atgttccacc aggagcgcgt cggcaaggac ggagtcccgt tccggctcca
    24361 caagctgcgc accatggtgc ccgacgccga ggaacggctc gccgacctca aggcgctcaa
    24421 cgaggcgtcg ggacccatgt tcaagatggc cgacgaccct cgggtgaccc cggtcggtcg
    24481 attcctgcgg gccacgtcca tcgacgagct cccccagctg ttcgacgtcc tgaagggcca
    24541 gatgagcctg gtcggcccac ggccggccct gccgtgcgaa gccgcgcagt gggacgagac
    24601 cctgtgggag cgcctgcggg tgcagccggg gatcaccggc atgtggcagg tgagcggccg
    24661 ctacacgacg tcgctcgaga cctacgcccg cctcgacctc tactacgtcg acaactggtc
    24721 gctggtgaca gacctgctga tcctggcccg caccgtcggc gtcgtcctgc gccgcaaggg
    24781 cggcgcctga acgacatcag ggcgtcgtcg gccgtccggc atcgttcccg tagaccggga
    24841 acccggcgca cagctcggcc acctcggatc ggaccgccgc gatctccgcg gtgtcaccga
    24901 cgtcgcgcag cacccgggtg atcagctcgg cgatcgtgac catctcggtc tcccccatgc
    24961 cctgggtcgt gaccgccggg gtgccgatgc gcaggccgga ggtcacgaac ggcgaccggg
    25021 gatcgtccgg gacggtgttc ttgttcaggg tgatccccgc gtcgtcgagg gcgttctgcg
    25081 cgtccttgcc ggtgaggtcg gcgtcgaagg tgcgcaggtc gatcagcatc aggtggttct
    25141 cggtccctcc ggagaccaag cggaaccccc gctcgccgag cgctccggcc agagccacgg
    25201 cgttggcgac gatctgacgg gcgtagtcgc cgaactccgc cgtggccgcc tcgcggaacg
    25261 cgaccgcctt gccggcgacc acgtgctcga gcggtccgcc ctgcagaccg gggaagatcg
    25321 ccttgtcgat cgccgcggcg tgctcggcgc gcgacacgat gcagccgccc cggggtcccc
    25381 gcagcgtctt gtgggtggtg aaggtgacca cgtccgcgta cggaaccggg ttggggtggg
    25441 cgctgccggc gatgagcccg gcgatgtgcg cggcgtcgaa caggaacagg gcgccgacct
    25501 catcgcagat ctcccggatc ggggcggggt cgatgatgcg ggggtacgcg gtggcgccgg
    25561 ccacgatcat cttcggccgg tgcgtccggg ccaggtcgcg cagctggtcc aggtcgaggc
    25621 gctcgtcgct cggggtcagc ccgtaggaca cgaagtcgta gaagcgaccc gagatgttga
    25681 ccggcgaccc gtgggtgagg tgcccgccgt ggtccaggct caggccgagc acggtgtcgc
    25741 cggcctcgag cagggccagg tagacgccga ggttggcgtt ggcgccggag tggggctgca
    25801 cgttggcggc ctcggcgccg aagagctcgc acacccgctg gcgggccagg tcctcgatct
    25861 ggtcgacgac ctggttgccg ccgtagtagc gcttggtcgg gtatccctcg gagtacttgt
    25921 tggtgaggac cgacccgacc gcgtccatga ccgcgggaga ggtgaagttc tccgacgcga
    25981 tcagctgcaa cccggtgttc tggcggtcga cctcgcggtc gatcatgtcg aagatctcgg
    26041 tgtcgggctg cgtgggccag ggcatggggg cgtcgttcct gttctgttgt gtcgggggga
    26101 ttgggggggt tcggtcgacc gggtcaggcg agtccggcgt ccagctcgga gatcttggcc
    26161 actcggccgg cgtggcggcc accctggaac tcggcgtcca gccaggcgtc gagcgcgtcg
    26221 cgggcgacct cctctccggt gaggcgctcg ccgatgcaga tcacgttggc atcgttgtgc
    26281 tcacgggcca ggcgggccga cgtctggtcg tgaacggtcg cggcccggat gcccggcacc
    26341 ttgttggcgg cgatggcgat gccgatgccg gacccgcaca cgcacagacc caggtccgcg
    26401 tcgccgcccg ccaccgccct gccgaccgcg gcgccgaagt cggggtagtc gacccggtcg
    26461 gtgccgtgcg tgcccagatc gatcacctcg tagccccgat cggcaaggtg accgccgagc
    26521 gcgtccttga ggtgcacccc ggcgtggtcg gcgccgatcg cgatccgctc ggtcacgatg
    26581 tggcctcgga gcgctcgagc cgctcccgca gcacggtcat gacccgttcg gacgccttgg
    26641 gcatctcgtc gagcagggtc cggaactcac gtgtcccgaa gcggagcagc ttcatcggtg
    26701 tgtcggccac gaccgtggct gtgcgcgggc gatggtcgat cagcgccatc tcccccacca
    26761 tcgaccccgg accggagctg gcgacgtgct cgccccggac gtacacgctg gcctgaccct
    26821 cgacgatcac gtagcactgc gtgccagggt cgccctggtc gaccaggacg gtgccggcgg
    26881 tggcctccat ctgctgggag aggtcgacga cccgatgcag gtcgtcgttc gagaacccct
    26941 cgaagaacgg taccgacgac agccaggcga cgtcgtcggt gctctttctg gtcatggtct
    27001 tccctcgttg cggacgggtg cgggcactgc gaacactcgg gcactgcgaa cactactgtc
    27061 gggtcggcgt gcacgtcggg gccgatcgcc gccgacccgt ccacaccagg gggaacgatg
    27121 agagcagtcg tcatgcgcga cggggcgctg gtggtcgacg aggtatcgct gccggcgctg
    27181 caacccggcc aggtccgcgc cgaggtgctg gcgtgcggga tctgcggatc cgatctgcac
    27241 gccctgcacc acgcggatcg catggtggag atgtcggagc tgaccgccga gcccggcggg
    27301 atcgcgcccc ggatgatgga cccgtcccgt gacgtggtga tgggccacga gttcaccgca
    27361 cgcgtgctcg aggtcggccc caacgtcggc aactgcacgc caggcgacgt ggtcgtgtcg
    27421 ctgccggtgg tcatggacgc cggcggcatc cacgccgtcg ggtactccaa cgactacccg
    27481 ggcggctacg gccagcagat cgtcctgtcg gacatgttga ccatgaaggt gcccgacggg
    27541 ctcgaccccg agctcgcggc gctgaccgaa ccgatggcgg tcggtctgca cgcggtcgag
    27601 atgagccgcg tgtcgccccg tcacgccgcg gtggtgctcg gttgcggtcc ggtgggcctc
    27661 gccgtggtcg cggcgctggc cctggccggg gtagagacga tcgtcgccgc cgacttctcg
    27721 cccgcccggc gggcgctggc cgccaccatg ggcgccaccg aggtcgtcga cccgtcggtg
    27781 gagggggcgg tcgccgcgtg gcgtcgcgtc gagggccgca aaccgttggt gatcttcgag
    27841 gcggtcgggg tcgaggggat gctcgacgcg gcgatggtcg acgcgccgcg gggctcgcag
    27901 atcctggtcg tcggggtgtg catggtcccg gaccggatcc gccccatggt cgcggtcggc
    27961 aaggagctga acctgcagtt cgtgctcggc tacgacccgc tgcagttcca gacgacgctg
    28021 aaccgcatcg ccgagggctc cctcgacgtg tccccgatga tcaccggccg ggtcgacctc
    28081 gacggtgtgg cccaggcgtt cagtgcgctc ggggaccccg aggcccacgc caagatcctc
    28141 gtcgtcccct cctgacccac cccgccctga cccgttctgt tcggcggttc acaacgtcga
    28201 cgttgtgaac cgccgaacag aacgggggtg gcgggcgacg agggcgcggt cgcggccggt
    28261 caggtcctgg gccaggaaca cctcgtcgaa gtgctcgccc gccaactcga ccgcggcggc
    28321 cccctgctcg ggtgacagct cgcagaccac gacgccgtcg ggaacgagcc acccgggcgc
    28381 ctcggccagg atcacgcgca ggtcgtcgag tccgtcggcc cccgcgaaca gcgcaccggc
    28441 cggctcccac tcggcgaccg acggcgacag gtccgcgttc gcggccacgt acggcgggtt
    28501 cgacacgacc aggtcgacgg ttcctcccag ctcctcgggc agcgcgtcga accactcgcc
    28561 gtgggcgatg cgcacccggg caccggcccg acccacgccg gccaggttcg cccgcgccac
    28621 ctcgagcgcg tcggtcgagg catcggtggc ccacaccgcg gtgcgcaccc gttcggtggc
    28681 gacggccagg gcgatcgctc ccgatccggt gccgaggtcg accaccgtcg tcggccggtc
    28741 cctccctccg atgcggtcga gctcggccag ggccacctcg accacctgct cggtctcggg
    28801 tcgggggatg agcacccgcc ggtcgaccat gaggtcgagg tgccggaacg gccagtgccc
    28861 cagcacgtac tgcagcggct cgccggtcgc gcggcgcgcc accatcgcgt cgaggtgtgc
    28921 gacgccgcgg cgggtggccc gccccgacag gtgcagcacc agctcggcat cggcaacgcc
    28981 caacgcctcg gcggtgatgc gcgccgcgtc cgtcgcggga tcggaaccgt cgccgggatc
    29041 gcggctgcgc agggtggcga tcgccccctc gagcagctcc cgccaggtga cggtgctggt
    29101 ctcatcctcg tcgctggagc ccccgctcac gtcagctccg ccagccggcg ggcctgctcg
    29161 tcggccacga gggcgtcgct gacggtcccg agctcgccgg ccagcacctt gtcgaggttg
    29221 tagacggtga ggccgatccg gtggtcggtg acccggttct ccttgaagtt gtaggtccgg
    29281 accttctcgc tgcgtccgcc gccgccgatc tgggcccgct tctggcccga cgcctcggcc
    29341 atcgccttgt cctgctccat ctgcagcagc cgggcccgca gcacccgaag ggccttggcc
    29401 cggttctgga tctggctctt ctcgtcctgc atcgacacca ccagaccggt cggccggtgc
    29461 gtgatgcgca ccgccgagtc ggtggtgttg accgactgac cgcccggtcc cgacgagcgg
    29521 aacacgtcga tctgcaggtc gttcgggtcg atgtcgacat cgacctcgtc ggcctcgggc
    29581 agcaccacga cggtggccga cgaggtgtgg atgcggccct gcgactcggt gacgggcacc
    29641 cgctgcaccc ggtgcggacc ggcctcgaac ttcatgcggg tccacacgcc gtcgccggcg
    29701 acccgcagca ccgcctcgct gaccccgccg aggtccgacg gcgacgccga cagcacctcg
    29761 acggtccagc cctggcgctc ggcgaagccc cggtacatct ccatcaggtc ccgggcgaag
    29821 aggttggcct cctctccccc ctcggccccg cgcagctcga cgatcacgtc gcggtcgtcg
    29881 ttggggtcgc gcggcagcag aaggacccgc agttcctgct ccagccgttc gatcgactcc
    29941 cgtgctgcgg tggcctcggc ccgccacgcc tcacgctcat cgccgctcga cgcggccagc
    30001 agctcctcgg ccgcggccag gtcgtcggtc gcggtccgca acgccaccgc ggtctcgacc
    30061 actggctgaa gcgcccggta ccggcgcgac atctcggtgt agcggcggtg gtccgcgacc
    30121 acctcgggat cggccagacg ggcctcggtg tcggccagct cccgctcgag cccggccgcc
    30181 cgggagagca gcgacccgtc ggtcacgact gctcccacgg cgccgggacg gccacgacct
    30241 ccgccggcga caccagggcg gcggcgaggc ggtcgagggt ggggatccgg tcccgctcgg
    30301 gtaccgccag caccagccgg gccgacgggt cgacccaggc ccgggtgtcg gcggcgacgg
    30361 gcacgaaggc caggtcggcc ccggtcgagc acaccaccac gacgggtccg cccgcggcat
    30421 cgatcccgac cgcggctgcc ggcgacgcct cccggacgct gtcgcgacgg accgtggtct
    30481 cggtcggctg gagtcgggtc gcgcccacga gggccgggtc ctcgaggatc gagcagcgca
    30541 gccagcgctc ccgacccaga cgcgacagcg gatgggtgcc ggcccccggg acgcgctcgc
    30601 ggcggaccat ctcgacggag cgggccagcg tggtcgtcgg cggctcgccg tggtgcatcg
    30661 ccgcgacggc gtcacggtcg aagcggccga ccccggcctc gaggtgcagc tcgccgtcac
    30721 caccggactc gacgggccag gtgaccaggc gggcgacctc gagcccgagg acctcaccgc
    30781 gcacgacgcc gtcctcgacc acgacctcca gcccggcgtc ggtcagggcc gtgaagagat
    30841 cggcgacggc cccgtccgaa gggtcgggga cgtcgagccg ctcggggaac ggggcgggcg
    30901 gcaccgggac ggaaccggcc ccctgcaccg accgcacctg caccgggacc gcgaaccacc
    30961 cggccatccg ggcgacgtcg ccgcccaggt cgtcggtgaa caacaccagc tccgccgcac
    31021 cctgacgcag cgtccaggcc aacgctccgg ccagcgacgc cggatcggcc acctccgaca
    31081 ggaggtacgc ggtcgctccg tcgatagcgg cggcgttggt gcccagcgag accacctcgg
    31141 gcgacgcgcc ggtgaggtcg cgcaccaggg cgccgagctg cgcccgccga agccgggccc
    31201 cttgttcgtc gctcagcacg acggcttcac cgctcggcga ccgagcggcg cggtcactcc
    31261 cccgcgtcgg cgggggcgtc ggccttggcg gccttgcggc gcttgccgta gcggcgctcg
    31321 aagcgctcga cgcgaccggc ggtgtcgacg atcttcatct gaccggtgaa gaacgggtgg
    31381 gtgaaggccg agatctccac cttggcgagg gggtactcgt tgccgtcatc ccacgtgacc
    31441 gtctcggtgg tctcgatggt cgaacgggtc aggaacttgt cgcccgagga cgtgtcctgg
    31501 aacacgacgt agcggtagtt cgggtggatt ccgggcttca tggtctcagt gctccgtgtg
    31561 ggtcccggtt tccgtgcggg accccgttga ggggacggat cagtatggct tctgggccgg
    31621 ggcgtcggcc accctggtgc tctcctaacc gggcacccct ggtgctctcc taaccgggca
    31681 cccctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccct ggtgctctcc
    31801 taaccgggca cccctggtgc tctcctaacc gggcacccct ggtgccttgg cgatctcggc
    31861 caggaagacg tcgttgttgg ggaacgtgcg catgcggtcg atgagcagct ccaggccggc
    31921 gccggtgttg ccctcggccg ccaggcccga cagcacccga cgcagcttcc agacctgctg
    31981 gagctgggtg cgctcgaaga gaagctcctc gtggcgggtc gacgacgcgt cgacgtcgat
    32041 ggccgggtag atgcgcttct cggccgcctt gcggtccagg cgcagctcca tgttgccggt
    32101 gcccttgaac tcctcgaaga tcacctcgtc catcttcgag ccggtctcga ccagggcggt
    32161 ggcgaggatg gtcagcgagc cgccctcctc gacgttgcgg gccgcaccga agaacttctt
    32221 gggcgggtac agcgcgccgg tgtcgatgcc gccggacatg atgcgccccg tggccggcgc
    32281 cgccaggttg taggcccggg ccaggcgggt gatgccgtcg aggatgatca cgacgtcctt
    32341 gcccatctcg accatgcgct tggcccgctc cgaggcgagc tccgccacct gggtgtgctc
    32401 gtcggagggc cggtcgaagg tcgacgcgat gacgtcgccg cggaccgacc gacgcatgtc
    32461 ggtcacctcc tcgggacgct cgtcgaccag caggaccatc aggtggacct cagggttgtt
    32521 gtgctcgatc gactgggcga tggtcttcat caccgtcgtc ttacccgcct tgggcggcga
    32581 cacgatcagg ccgcgctggc ccttgccgat cggagcgatc agatcgacga tccgcgacgt
    32641 catgtcgccg ggcaggtcct cgcgctccaa gcggaggcgc tcgtcgggga acagcggggt
    32701 caggtcctcg aaacgcggac gctcgcgggc cttctccggg tcgccgccgt tgaccttgtc
    32761 gatgcgcagc agcgccgggt tcttctcgcc ccggccggcg ggacggctgg caccctcgag
    32821 gaagtcgccc ttgcgcagac cgagctggcg ggtctgcttg accgacacgt agacgtcgtc
    32881 gcggctgggc aggaagccct tgacccgcag gaacccgtag ccctcgtcgc gcaggtccag
    32941 gaagccggcg acctcgacgg gctcaccgtc ccagttgtcg ttctgcggct ggccggcgtc
    33001 ctcgccctga cggtcgcggt cacggccccg acggcggcga cgacgacggt tgcccggctc
    33061 gggctcaccc tggttctggc cgccctggtt ctggttctgc tggttctgct ggttctggcc
    33121 gccctggttg cggttctggc cgcccttgtt ctggccaccc tggttctggc cgccctggct
    33181 cttgccaccc tggctcttgc cgccctggct cttgccgccc tggtcctgat cctgatcgac
    33241 ctggccctcg ggctcgttct cgggcttgct ctcgggctcg tcggaccggt cccggtccga
    33301 cggaccctgc ccgccgtcgg aggcctcggc atcggcctcg gccggggcgg cctcggtgtc
    33361 agcacccgcg gcaccgttgc gcccgttgcg ggccggggct gcgggcgcgg ccggagcagc
    33421 gtcgggaccg gccgaggtca ccccggccag gtccaggatc atgccgatga tgtcggcctt
    33481 cttggtgcgg ctcgtgggct tgccgcccaa ggcctcggcg atcgtggcga gctcggcgcg
    33541 gtccttgcgc tcgaggccgt cgacgtcgat cgaatcaaga ctcacagtgg tgatgtccct
    33601 cgtggtcggt gcagcggcgt ggatcgcggc tgcgctgggg tggatcggga cccgacgaac
    33661 gggattcgat ccggggtcga ccggagtgcc ggggaccgga gaggtcgggg gggaccccac
    33721 cgagcggtgg gcgggactgg gcggtggttc gacagcgacc gaacatcgat ggttcgggat
    33781 ctgccggacc tcagcccccg ccggttcgac atggccacac tacccgccgc atgtggatcg
    33841 ggcaaccacc cgggcgctca ggcagcgccg gcgccgatca gccggccgag cagctcgtga
    33901 ccgggcacca ccggggtgtc cgccacacct gcctcggtgt aagtgccccc cggcccgtcc
    33961 acggcgctgt cgaccagcag gtagggcacc ggatcgggcg tgtgggtgcg gcgggccagc
    34021 ggggtcgcgt gatcgggcag catcagcagt cgccacggcc ccatgcggtc gagctccgga
    34081 accagaccgg ccaggatctg tgagtcccac agctcgagcg agcggacctt ctcggccgcg
    34141 tcgccggcgt ggccggcctc gtcggtggcc tcgacgtgga tgacgaacag gtcgagcccg
    34201 tcggccagcc cggccagcgc ggcgtcgcgc tgcgcggcgt agtcggtgtc gtagccgggg
    34261 gtggcgccct cgacgtcggg cagggccatg tcggtcagca cgcccagtcc ccggaccagg
    34321 tcgaccgcgg tggtgatgcc gccggtgatg ccgtgatgcg cggcgaagtc cggcatcgac
    34381 ggctggaacc cctgacccca caaccagatc tggttggcgg ccacgtcggg atgacgggcc
    34441 agaacggcgc ggctggcctc ggtgacctcg atcagccgtt cggccgccgg gccgctggga
    34501 gcgaccgcgg gccggccggt caggtcgtgc ggcggcacgc actcggcgtc gagccagtcg
    34561 gcgggggcga ccatgatgtg gcggtactcg accccggcgt ggaactcgag gaccgagtcc
    34621 gggttgcccc gtccgagcgt ggcgtcgaga tcctcgagca cggcccgggc ggcgtcggtc
    34681 gacgggtgcc cgccggcgaa gtcgaccatc gtggcgtcgt cgccgacggt gaccaggttg
    34741 caccggtagg ccaggcggtc cgccggcagc ttgagcccga gcgacgcggc ctcgatcggg
    34801 gcgcggccgg tgtggaactc ggcggggtcg tagcccagga tcgacatgtt gccgacgtcg
    34861 ctgcccggcg ccatcccctc ggggatcacc ccggcccggc cgacggtgcc gcgcgccgcg
    34921 agcgcgtcga tcgtcggggt gtgggcgacc tcgagcgggg tgcgaccgcc caggccctcg
    34981 aacggctcgt cggcacaccc gtcggggacg cagaccacgt atttcaccgg acgctctcca
    35041 tcatcggccc agtctggccg accgcgttcc cctccccgaa ctcgggcacg cacttccacc
    35101 cgagtgccgg cgatgttgta gccaccgcgc caccacagcg acgggggcct acaagttcga
    35161 cgggcagcca cgaccttgta gccaccgcgc caccacagcg accggggcct acatgttcga
    35221 cgggcaggtc agccggcaat ggcgcggcgg accagggcct ggaccgaggc gaggtcggcc
    35281 gggcagtgct cgacgcgctc ctcccgctcg aacaggtcgc ccagcgcgtc gggcagagcg
    35341 ggccggacgc cgaccgcggc ctccaccgcg tcggggaact tggccgggtg cgcggtggcc
    35401 aggcagacca cgggaaccga agggtccgtc aggacaccgg cgccggcgag cgcctcggcc
    35461 accccgatgc cgaccgcggt gtgggggtcg accaagagcc cggtgtgctg ctgcaccgac
    35521 cggatcaccg cggcggccga ctcgtcgtcg aggcgaccgc agtcgaactc ggagcgcagc
    35581 tcggcgagca cctcggaggg caccgacacc gacccgtcgg cgcggaaccg ggccatcaat
    35641 tcggccaacg cgacgccgtc gcgtccgagc acctcgaaca gcaggcgctc caggttggag
    35701 ctgacctgga tgtccatcga cggcgacgtc gtgggcacca cctcggtgat ggccatggtg
    35761 ccggtgctga agaagcgggt gaggatgtcg ttgcggttcg acgccaccac cagccgctcg
    35821 atcggaaggc ccatgcgctt ggccacccat ccggccagga tgttgccgaa gttgccggtc
    35881 ggcacgacga agctgaccgg tcccccgtcg ggagcgacgg ccaggtgcgc ggtcacgtag
    35941 tagacgacct gggccatcac ccgcgcccag ttgatcgagt tgaccgccga gaggtgcacc
    36001 tcatcgcgga acgcctcgtc cgcgaacgcg gccttgacca ggtcctggca gtcgtcgaag
    36061 gtcccgggca ccgcgacggc gtgcgcgttg gcggctccga gggtggtcat ctgccggcgc
    36121 tgcacctcgg aggtccggcc gtcggggaac aggatgacga tgtcgacgca gtcccggtcc
    36181 gcgcacgcgt gcatggccgc cgagccggtg tcgcccgagg tggcgccgag cacgcagacc
    36241 cgctcgcccc gggccgacag cacgtggtcg aacagccgcc cgaccaactg cagcgccacg
    36301 tccttgaacg cgagcgtcgg accccggaac agctcgagca ggtgcaggtc gggcccggtc
    36361 tccccaccgg tcgagcccag cgggacgacc gggcacacgt cgggggtgtc gaaggtggcg
    36421 taggcctcgg cgacgatcga ctcgaacgac tcccggtcga gtgcgcccgg aacagggcgg
    36481 ccctcggcgt ccacgtcacc gtcgacgaac ggccacatga cccgcgtcgc gacctccccg
    36541 tagctcaggc cgcgcagctc gtcgaggggc ggcagctcgg gccagcgttc gggcaggtac
    36601 aggccgccgt cgtcggccag gccggcgagc agcacgtcgg agaagccgag cacgggcgcg
    36661 gcaccgcggg tcgagacgta gcgcacaggg cgatccggtc agtccgagcc gatgacccgc
    36721 agcacggagc cgaccgagcg cacggcgtcg aggccgcgca gctcgccgag ggtggatcgc
    36781 aggtcggcct cccgggcggt gtgggtgatg aaggtgaggc gggcgcccga atccccggag
    36841 tcgagcgact cctgctccat cgagcggatc gacacgccgt gggcgccgaa cacacccgcg
    36901 accgcggcga ggacgccggg acggtcctcg acgaccaggt tgaggtagaa cgcggatcgc
    36961 agctcgtcga tgggccggat gcgggccttg ccgaacgagc cgagcgacgc gtgggtgccc
    37021 ttggccaggt tcgccgccgc gtcgatgacg tcgccgagca ccgcggaggc ggtcgggaac
    37081 ccgccggcgc cgcggccgta gaacatgagg tcgccgacgg cctcgccctc gacgaacacg
    37141 gcgttgaagc tgtctcgcac cgacgcgagc gggtgggcgt cggggaccat cgcggggtgc
    37201 acccgcaccg cgaccgaccc gtcgccgtat cgctcggcga tggccagcag cttgatctgg
    37261 tagccgaggc ggcgggcgtg gtcgatgtcg gacgaggtga ccccggagat gccctcgtgg
    37321 aagacgtcgc cggcgacgac cttggcgccg aaggcgatcg tggcgatgat cgcggccttg
    37381 gccccggcgt cgaagccctc gacgtccgcg gtcgggtccc gctcggcgta gcccaggctc
    37441 tgcgcctcgg ccagggcctc ggcgtagctc cagccctcct cggtcatctt ggtgaggatg
    37501 tagttggtgg tgccgttgac gatgcccatc acccgggcga cgtcctcgcc gacgagtgac
    37561 tcgcgcagcg gccggatcag cgggatgcca ccggcgaccg cggcctcgaa gaggaggtcg
    37621 acgccggagg actcggccac cgcgtagagc tcgccgccga cgttggcgag cagctccttg
    37681 ttggcggtga cgaccggctt gccggccttg agggcgtcga cgatcagctc gcgggccggt
    37741 tcgatgcctc cgatgacctc gacgaccagg tcgacgtccg ggtccgcagc gaccgactcg
    37801 gcgtcggtgg tgagcacctc ggccggcagc tcgacgccgc gctccttgga cagcgaacgg
    37861 accgcgaccc gggtgaccgc caggcgaagg ccggtgcgcg ccgcgatctc gtcgccgcgg
    37921 tcgcggacca gttcgaccag cgccgcgccg acgttgccgc agcccagcag gccgatgttg
    37981 acggttccct cgctcaccgc gactctctcg ctcaccgtgc ccaggctacc ggcgcaccgc
    38041 cggcggtctc atccgacatc cggtcaaccg acatcggtgg ccagcaggtc ctcgacggtc
    38101 tctcggcgca ccaccagccg gtcgacgccg tcggcgacga acaccactgc gggacgcagc
    38161 accttgttgt agttggagcc catcgagtgg ccgtaggcgc cggtgaccgg ggtggcgatc
    38221 acgtctccga cggccaggtc cgcgggcacc cggccctcgc gcaccagcag gtcgccggac
    38281 tcgcagtgct tgccgacgag gcggatcggc atctcgcgtc gcgacgtcgc ggcccgggga
    38341 aggaacgtct cgtagcccga cccgtacagg accggccggg ggttgtcgct catgccgccg
    38401 tcgaccgaca cgtaggtgcg cacgccgggg atgaacttga cggtgccgac ggtgtagagg
    38461 gtgaccgcgg cggcgccgac cacggcgcgt cccggctcga ctccgatctc ggcggtgatg
    38521 ccggcggcgc ggcactcctg caccacggcg gtggcccact cggtaatcgt gggggcggcc
    38581 tcgccctcga cgtacgcgac gccgaggccg ccgccgacga ccagctcggg gaggtccgcg
    38641 gcgacgacga agggggccac ggcgcgcagg ccctcgagga agttctcgac gctgaacacc
    38701 tgtgagccga tgtgcatgtg cagcccgacc aggtcgaccg cggcggatgc gcgggccctg
    38761 gcgagcgcgt cgtcggcctg gccggtgggc aacgggaacc cgaacttgga gtcgaggttg
    38821 ccggtgcgga cgtgctcgtg ggtgtggacc tcgatgccgg ggttgatccg cagcagcacc
    38881 ctcggcacgg gcagacccga ggcgaccagg gactcgatgc ggtcgagctc gtcgtcgctg
    38941 tcgatgacga tgcgcccgac gccggcggtg agcgccaggc gcagttcgtc gagcgacttg
    39001 ttgttgccgt gcaggacggt gcgctcggcg ggcacacccg cggcgaggac gacctgcagc
    39061 tcgccgccgg tggcgacgtc gacgcgcatc ccctcctcgt gcaccagccg ggcgacggcg
    39121 cggcacagga acgccttgga ggcgtaggtg gcgccgtcgc cgagcaccga caccgcctcg
    39181 cggcaacggg cgcggatgtg ggcctcgtcg tagacgaaca gcggggtgcc gtggcgatcg
    39241 gccaggtcgg ccacgtcgca gccgccgatc gacagccgac cgtggtcgtc gaccgtggcg
    39301 gtgtcgggca gcaggtggaa cggcagcggt gagttggtcg gcgcgctcac atgcgctccg
    39361 gggccgacac gccgaggagg cccagtccga cgcgcagccc caccccggcc gcctcggcca
    39421 gccaccagcg ggcctggcgc agctcgtcgg gcgtgtcggg gtgaaggatc gggcagtcgt
    39481 ggtagaagcc gtggaccgag gaggccagct cgcgcaccca ggtggtgacc ttgtgcggcg
    39541 cccgctcccg ggccgcgagc agcaccacct cgccgagcac gtcgagttgt cgcagcacct
    39601 cgagctcgcg ctcggcggtg agcagcgaca ggtcgacctc ggccaggggg cggcgttcga
    39661 tgccccgctc ggcggcgacc cgggcgatcg acgcgatccg ggcgttggcc atctgcacgt
    39721 agaagaccgg gttctcgttg gactgctgga cgatcagtcc cagatcgacg gtctggggcg
    39781 agtcgaccga ctgcagcagg taggtgaggc gcacggcatc ggcgccgacc tcgtcgatga
    39841 ggtcctcgag ggtgatgagg tcgccggccc gcttggagat cttgacctcc tcgccgtcac
    39901 gctccagtcg gacgagctgg gtgatgacga cctcgaggtc ggccgggtcg tggccgaggg
    39961 cctggatggc agccttcatg cgcggcacgt atccgtggtg gtccgcgccc cacacgttga
    40021 tgaccaggtc gaagtcgcgg gcgaacttgt cgcggtgata cgcgatgtcg ggcagcaggt
    40081 aggtgtactc gccgtcgctc ttgacgagca cccggtcctt gtcgtcgccg aacacggtgg
    40141 agcgcaacca gaccgccccc tcgcgctcct cgaccatgtc gcgctcgcgc aggtcgtcga
    40201 gcgtgtcggc gatggcgccg gacgcgagca gggaacgctc cgagaaccag gtgtcgaact
    40261 cgacgccgag gcgggcgagc acccgccgtt gatccgcgag cgcgtgggcc tcgccccact
    40321 cgagcggatc ggcgccgtcg ggcatctcgg cggcccagtc gacgatgtac tggccgcggt
    40381 agccgtcctc gggcggctcc tggccggcct tgcgggcggc gagcgacgcg gcgaaggtct
    40441 gcatctgcac gccgcggtcg ttgacgtaga actcgtcgga gacgtcgtgg ccgacgaagc
    40501 gcagcagccg ggccaggctg tcgccgtaga ccgcgccgcg ggcgtgaccg gcgtgcaccg
    40561 gaccggtcgg gttggccgac acgtactcga ccatcacccg ggtgccggcg ccgacgtcgc
    40621 tgcggccgta gtcggcaccg gcgtcgacga cggtgccgat caactcgtgc agccacgccg
    40681 ggtccaggcg gaagttgacg aagccgggcc cggcgacctc gaccgcggtg acgtgacggg
    40741 gcgggtccgc ggtgagcgcg tcggcgagct gctgggcgag ctcacggggg ttgcgcccgg
    40801 cggccttggc ggtggccatg gcgacgttgg tggagaactc gccgtgctcg cgccgggccg
    40861 gtcgttcgag gtggatctcg gcgggggcgt cgacgccgag ggcgccgagg gtgtcccgga
    40921 gggcggcggt cagctcgtct cgcatgagtc ggcgatcgta ccgccgggcc c
//
LOCUS       DAOQOD010000008        36490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_372_length_36490_cov_13.784383, whole genome shotgun sequence.
ACCESSION   DAOQOD010000008 DAOQOD010000000
VERSION     DAOQOD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 36490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36490
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_36490_cov_13.784383"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLZ72_01965"
     CDS             complement(<1..827)
                     /locus_tag="PLZ72_01965"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNA35091.1"
                     /translation="MVHSFAAVGVAKVRLTGGEPLLRRGLPDLVSRIATVDGIRDLAM
                     TTNGTLLAPVARDLRVAGLRRLSISLDTLRPERHHEITKRDSHSAVIAGIDAAADAGF
                     TGTKINTVVTRGVNDDELTDLVHFGAAHRAEVRFIEYMDVAGASRWDERLVVSRSEIL
                     ATVGAALGPVTALPTRPSAPARRYRAGDGTAFGIVASTSTPFCASCDRSRVTADGLWY
                     HCLYAATGTNLRTPLRRGATAEELRDLVACGWARRVDQGAVDRLRLAERRAATPVEVT
                     "
     gene            complement(872..2557)
                     /locus_tag="PLZ72_01970"
     CDS             complement(872..2557)
                     /locus_tag="PLZ72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HNA35092.1"
                     /translation="MARQSSRGRGPASGRQAPQAQGMSSSSTPIDRRTALGLVGGAGA
                     LVALTACGGGNGGSSTSTTASSVVKAGTAVVPEGMPQLEAEVRDLHLRLVAAPATAEI
                     VAGTPTDVLSFGAEVIDGDPASVTPSGSYLGPTLHVRTGQRVQVIFENQLSQETIVHW
                     HGLTVPQEQDGQPLEAVGPGETYEYDFTVVNEPGTYWYHPHPHHLTGEQVYRGLAGLL
                     IVHGDEPETTLPHGARDLGFVLQDRTIGADGQLVYVASNHDVMAGFVGETLVTNGVAG
                     LATSVERAPYRLRFLNGANSRTQILTLSTGEPLQVVATDGGLLPEVANVPGLVLTPAQ
                     RSDLWIDFSSYEPGQQIDLLTAGTFVEVPGMMGGGGKGNGGMGRGGIGGGAALTLNPK
                     VAMSFVVADGPAEPGEVPQGLAGQVTIEPSEAVNTDTPRVFELMTRQASHWINGMQWE
                     GRTAAEWETVAFGTTEIWEFVNLSPMAHPMHLHGKAFRVVDRSWVNASGEESWAQISS
                     GIVETGTRDTVLVWPGQRVRIAVSFDTYRGYFLYHCHILEHEDGGMMRNFLIT"
     gene            complement(2640..5243)
                     /locus_tag="PLZ72_01975"
     CDS             complement(2640..5243)
                     /locus_tag="PLZ72_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35093.1"
                     /translation="MTRRVLRQLWISRSRYLLTFLAIVMGVAFVNATLTLTDSIGAGD
                     EATVEAGEAGIDAVIDAVPGTPPAGFSGPAEMVSAGIGIPTSVLGTVTADPDVAAAQA
                     VWHANASLLSADGSIIGQSRGPFNEAETWITDSETARWHIAEGRAPAKPGEMALDAAT
                     ARDFGVVLGDEYRLATERSSTPLTVVGIARYGPADRLPSRTTVLVAPDEPALIGQGDV
                     ATQIVVRGVPGVSPDVLVDGLRADLAQSGETVRVRCGDAAAEAELARIAVESTILSRL
                     LTVFTIVAALTGMLIITNTFAISMVQRRRELALMRAIGNTRSELVRSVLTEAFILGVA
                     ATALGLVLGRFVVVLIQLLFDRAGVEAFNGPVIFTAGTLATTIFIGVVITLLSALRAA
                     VSGSRVPPAAALRDAAVDESSAPWWRRGLAPVLVVIGVAATSAGIASQRNQLMLAGTL
                     LAVIGIYLAGPLFASLAAGAARPVLAVVAGATGRIAARNAARSPRRVSSAAAGLMIGI
                     AVVAFFSILAGTVKTLQVGPTSALRADHVVTPLGAPAGKVPGDLTPELRAVPGVNDLA
                     AIHVTGGLLADPSEATTATPNAIPVGMIEADNLDAVYDLDAVYDLDAVYDLDATGADA
                     GHLEPGQMMVASSELGAHPVGSDLTIRGVAGVAKGTVVGSFATALPGFASPKVLLDQD
                     TYSAVFSDPGTVAIFLATNGQQATLDAVARATNGSGRFQTAEEYAGASSSPVDTILNL
                     IYALLGIAVVIALVGLANTMALSIRERTAEIGVARAIGTTRAQVVSSVLLEASITTIL
                     GVAMGLTIGIGVSFPTVTLLDNAAITSPVIPIARLVVIALLGAVAGTIATIAPALVAA
                     RRSPLESITAL"
     gene            complement(5240..5977)
                     /locus_tag="PLZ72_01980"
     CDS             complement(5240..5977)
                     /locus_tag="PLZ72_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35094.1"
                     /translation="MTDETPSVALTAATKVYGSADAEVRALDDVTIAFPRGQFTAVMG
                     PSGSGKSTLLNCMAGLDRLTSGTVEIGGVDLRDLSDRDLTILRRTRLGFIFQSFNLLP
                     ALTARENITYPLDLSRSRVDPQWFDELIGVLGLRDRLDNRPSQLSGGQQQRVAVARAL
                     VSKPEVVFADEPTGSLDSHTSGEILSHLRKASDLGQTIVMVTHDPYAAAHTHNVVFFQ
                     DGAPVGELASPTADGVLDRMKSLGADR"
     gene            6055..6633
                     /locus_tag="PLZ72_01985"
     CDS             6055..6633
                     /locus_tag="PLZ72_01985"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35095.1"
                     /translation="MSPPGETQPDPRITATRSRVLAAVSSLLESGGPSEVTHSSVARA
                     AKVGRATVYRHWPRIDDLLADAIETRLGVTPPGTTGNALTDLRSMLLAIVAGVFSDDS
                     AVFFTTLLVQGGDTPQMDALRREIIERRMGAIRDLVERGAIDGSIDPTLDPDRAISYL
                     AGPIMHRRIVLGQPVTPDFIDDLLLRVAATPP"
     gene            7100..7732
                     /locus_tag="PLZ72_01990"
     CDS             7100..7732
                     /locus_tag="PLZ72_01990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA35096.1"
                     /translation="MPALHLGDEAPDFTAMTTDGEISFHDWKKGSWAVFFSHPADFTP
                     VCTTELGRTAALQSEFAKRNTKAMALSIDPIEEHHKWAPDIGEVTGTPLNFPIVADPD
                     HRIAELYDMIHPGEGDTSTVRSVFIVDPDNKLRLMLTYPKSVGRNFDEIVRVIDALQA
                     TDANPIATPADWRPGDRVIVAMAVSTEDAKKQFDNVEEFKPYLRYADAPQ"
     gene            8037..9260
                     /locus_tag="PLZ72_01995"
     CDS             8037..9260
                     /locus_tag="PLZ72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA35097.1"
                     /translation="MNGYVAQRRGRFYAVIYEGRDPVTGKEIRRWHPAGTDRAEAEKL
                     AAKLAAAETKRVGAVRSLTFGAYLTSQWLPAKRLHLATSTYRGYERNVQRHILPVLGR
                     TSIRRLRYQQIESLYEALLHPETGRGLAPKTVYEIHLVIRVALADAHRRGLVTRNVAL
                     VARAPKQRSLQRIEGVSLTDEELRQLLRTAAGHRFFPIYWLTAMTGMRRNEVLGLKWS
                     DLDATKKRLHLNRGLVAVGYEVHQTRGKTKTARRVIELDATTIAVLEGWRAYQAAEFA
                     AVGISESDDWVFTDGDGEPVHPHAVYEAFRRIVHNAGVPTMRFHDLRHTHGSLLIKEG
                     VPVKVVSERLGHAHIAHTLETYQHVLPGMQEDAARTAERLADPRPTAKKPKNPTKRPG
                     KTPVERRGNNRRKAA"
     gene            complement(9752..10213)
                     /locus_tag="PLZ72_02000"
     CDS             complement(9752..10213)
                     /locus_tag="PLZ72_02000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNA35098.1"
                     /translation="MLAFTALAVLVVDVTSKVAASTWLRDAPVELPGPLDLQLAYNPG
                     AAFGLGSNLPLAVVLAVTTTVAALIAIGAWRGWLPNPWAAGLIVGGAIGNVIDRAEAG
                     TVVDMLHTGWWPTFNLADVAIVCGAMLLAFSSLRSNEPDHQRGHGDPGDAA"
     gene            complement(10300..12234)
                     /locus_tag="PLZ72_02005"
     CDS             complement(10300..12234)
                     /locus_tag="PLZ72_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNA35099.1"
                     /translation="MSATPTPVAPTRTEGEGGGLLGDLWRSREVRWSGFSGLLIAAGY
                     LAGPLGAVDAISTALFIAAALVGARFFAAEAVEELVRERAIGIELLMTVAAVVAGVLG
                     AWSEAAALAFLYSISESLEEFTEDRTRDAVRKLLDLAPRRVTLLGADGSEREVDVDEV
                     VVGDRFLVRPGQNIATDGRVLEGTSSVDEAAVTGESVPVDKMPGELVFAGTSNAQGAL
                     VVEATATSATNTLARVVELVSEAQERKGRGQQFMERFSKVYSPAVLALAAGVWITGGA
                     VTGSWNDWALRAATVLVAAAPCALVISIPVTYVAAMGRSSRSGILIKGGIYLEELGRL
                     RAVALDKTGTLTRGKPQLTELHPLRDLDADRALAIAASAERRSEHPIATAIVDAAEAK
                     GLDFSEPEDFRAAVGGGVHARIKGKQYTVGSPRFITDEGHDLGPIADMIEEMEVAGNT
                     TVVLVDDQGPVAVLGVADTVREQSADTITRLRGLGIDHVVMLTGDNPRTAAAIAAQVG
                     IDDVAAGMTPEAKAAHVQRLVEQYGHVAMVGDGVNDAPPLAAASVGVAMGTAGSDIAL
                     ETADVALMADDLSKLVTAIEIGRHTRRVVTQNLALSLVILATLVPLALFGVIALPAAV
                     LAHELSELVVILNGTRMARK"
     gene            complement(12231..12554)
                     /locus_tag="PLZ72_02010"
     CDS             complement(12231..12554)
                     /locus_tag="PLZ72_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA35100.1"
                     /translation="MQPDIAIAAHMFRSFGDPTRLAILTELLRGERRVVDLVDVLGCS
                     QSNVSGHLACLKECGLISDRPEGRSVHYRLATPAVVDLLRQAEALLGEIGERVELCEN
                     YRTKR"
     gene            12668..13240
                     /locus_tag="PLZ72_02015"
     CDS             12668..13240
                     /locus_tag="PLZ72_02015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35101.1"
                     /translation="MSRVERAFCRSAPWQSFTGRVVLPWLLGDTELDGQVLEIGSGPG
                     ANGRALLERYRAVELTATDLDPVMVAAARRRLAPHGARVTVTKADATDLPFDDDHFDT
                     AVSLLMLHHVIDWRDALVEVARVLRPGGTLVGYDLTDRRTARIVHLVDRSPHRLLASG
                     ELADGLDAAGFTAVQIDTAALGTVTRFRAE"
     gene            13323..13982
                     /locus_tag="PLZ72_02020"
     CDS             13323..13982
                     /locus_tag="PLZ72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA35102.1"
                     /translation="MSTDRTIAFVDLAGFTALTETHGDLAAVDLIDMFTGIATTAIEG
                     TGTELVKTIGDAVMLAAATPDSAIIAVQRLFEAAYDLGGFPEPRAGLHHGSVVARGDD
                     FFGATVNLAARVASRAGSGQALVTNSMLSAAHDADIDTVSLGLHQLRNILEPVELWAL
                     ELCPTHIDLSVDPVCRMRLACQAAIGRVRHHGVEYRFCSLPCTAAFAADPDRYLDRGA
                     S"
     gene            complement(14281..14637)
                     /locus_tag="PLZ72_02025"
     CDS             complement(14281..14637)
                     /locus_tag="PLZ72_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HNA35103.1"
                     /translation="MVVYRTPTCGCCKSYEDYLRKHDFDVQSEVRDDLTPIRSQNGVP
                     SEAASCHTVLLDGYAVEGHVPVEAIDKLLAERPAVDGIGIPGMPTNAPGMGEPDGRPL
                     EVLSFNDDGVAPFATL"
     gene            complement(15086..17065)
                     /locus_tag="PLZ72_02030"
     CDS             complement(15086..17065)
                     /locus_tag="PLZ72_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNA35104.1"
                     /translation="MFRRRFWISLILTVPLVVTSHMVMDWFGYTLDFPGIEWVGPVLG
                     SIVFLWAGWPFLQGGVREIRDRQPGMMLLISMAITVAYAASMATSLGWFDLDFWWELA
                     ALVTIMLLGHWQEMKAIGQAQNALAALAELIPDEAEVVRGEGVTETVPIDTLAVGDVV
                     LVRAGGRVPVDGEILDGEAELDESMITGESRPVEKREGDRVVAGTVSTDSSLRISVVA
                     VGDDTALAGIQRMVADAQESSSRAQVLADRFAALLFYVAAGAALATFVAWAALGDVDS
                     AVVKTVTVLVIACPHALGLAIPLVIAISTAVSARAGILVKDRLALERMRTIDAVLFDK
                     TGTLTKGAHVVTGVAAADTVDEDRMVAIAGAVESDSEHPLARAITAEAARRGDVPAAT
                     DFRARTGRGVDAVVDGEQYSVGGLALLRELGAPVPGEVQRRTDEWVARGAAALYLFHG
                     DGIIGAFALEDEVRPEARQAVDELKAMGIQVVMITGDAQQVADAVGAELGLDRVMAEV
                     LPEDKDRAVSELQAQGLSVAMVGDGVNDAPALARADVGLAIGAGTDVAIESAGVVLAS
                     SDPRSVTGVVRLSRASYRKMVQNLAWAAGYNIVAIPLAAGVFAWAGLDLSPAVGAILM
                     SLSTIVVALNAQLLRRVDLQLDPIAEEEVTSASLD"
     gene            complement(17157..19511)
                     /locus_tag="PLZ72_02035"
     CDS             complement(17157..19511)
                     /locus_tag="PLZ72_02035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNA35105.1"
                     /translation="MTATTHRTDEGTPDSAVRTLDLTISGMTCASCANRIEKRLNKVD
                     GVTATVNYATERAAVTIDGDVDVDPAELVAEVEAAGYTAQLPQAPGGEGDDNEGAATI
                     DPTAALRTRLLVSLALTIPVVAMAMIPALQFDNWQWLSLTLAAPVVVWGALPFHRAAW
                     LNLRHGTATMDTLISIGTLAAFGWSIYSLFIGDAGVVGMTMPFELTIQRGSGADEIYL
                     EVAAAVTTFILAGRYFEAKSKRRTGAALRALLDLGAKDVVVLRDGREERIGIDQLQVG
                     DRFVARPGEKIATDGIVDEGSSAVDASLLTGESVPIEVVPGDHVTGATVNAGGRLVIR
                     ATRVGADTQLAQMARLVTQAQTGKAEVQRLADRVSAVFVPIVIVLALATLGFWIGAGG
                     GTTAAFTAAVAVLIIACPCALGLATPVALMVGTGRGAQLGILIKGPEVLESTRKVDTV
                     VLDKTGTVTTGKMTLVDVVVEPGTTREDALRIVGALEHASEHPIAQAIANGAADEVGE
                     LPPVESFANREGLGVEGVVEGHGVIAGRPALLADWGLTPSETLLAEKAEAEAQARTAV
                     LAAWDGQVRALFVVADTVKATSAEAIQQLRDLGLTPILLTGDNTAAARSVAAQVGITE
                     VIAEVLPSDKVDVVQRLQADGKVVAMVGDGVNDAAALAQADLGLSMGTGTDAAIEASD
                     LTLVRGDLRAAPDAIRLARRTLATIKGNLFWAFAYNVAALPLAAAGYLNPMIAGAAMA
                     FSSVFVITNSLRLRRFAPLSASAGAAGRTPEIRSEGGHHVVDAA"
     gene            complement(19508..20515)
                     /locus_tag="PLZ72_02040"
     CDS             complement(19508..20515)
                     /locus_tag="PLZ72_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35106.1"
                     /translation="MNVPTKLAAFGALSAAVFFAALGVGAAVGPVDTQNQPTTHTPSI
                     DGAHDTAGGDEMVAMDHTATTLGGLETSQAGYTLVPTETALEATDTADYRFTIEGPDG
                     LPVNEYTKEHDKELHLIVVRRDLSGYQHLHPTRDAEGVWSTTTDLSVPGTYRILADFT
                     PTASGDGLTLGLDLQVPGEQRATPLPAPSRTAEIDGYTVTLTGDLVPGSSSALTPSVA
                     KDGRPVTNLEPYLGAYGHLVALRSGDVAYLHVHPEGEPGDGTTTAGPTIEFAASVPSA
                     GTYRLYLDFLHDGVVRTAEFTATASTDAPAAAAPDSSTTTPTSASHDDSDGHHSNGAG
                     R"
     gene            complement(20512..20721)
                     /locus_tag="PLZ72_02045"
     CDS             complement(20512..20721)
                     /locus_tag="PLZ72_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNA35107.1"
                     /translation="MSTTRKINVDGMSCGHCVTSVTNEVRKVDGVTGVDVDLAAGTVQ
                     ITSDAPLEEADVNAAIDEAGYEVRP"
     gene            20828..21112
                     /locus_tag="PLZ72_02050"
     CDS             20828..21112
                     /locus_tag="PLZ72_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNA35108.1"
                     /translation="MTERGYTATKEQLQARLSRIEGQVRGVKGMVDDDRYCIDVLTQI
                     SAIRSALDKVALGLLDGHTRHCLADGHGGPTDPDEQADELMSAVGRLLTR"
     gene            21614..22231
                     /locus_tag="PLZ72_02055"
     CDS             21614..22231
                     /locus_tag="PLZ72_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HNA35109.1"
                     /translation="MTSRQIRRAFSVAILAVALVTTAAACSNDDGTTGNSGATTTRGQ
                     TATAELPDDVNDADVAFVQGMVPHHEQAIQMSELVIANGDDPTVIALAEQINAAQGPE
                     IDQMNTWLDDWDIDPAGSDGMDGMDGANGMMSDDEMDMMSEAMGADLDRMFLETMIRH
                     HQGAIRMAEVELEEGSNADVLALAQGIIDGQQAEIDQMQSMLEDT"
     gene            complement(22308..22781)
                     /locus_tag="PLZ72_02060"
     CDS             complement(22308..22781)
                     /locus_tag="PLZ72_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNA35110.1"
                     /translation="MDDRTQRFTTTVVRDPRRRVLVPLPFDPDDAWGRKPAHPVAGTV
                     NGMAVRAVIETLDERSAILLGPAWRRDCGIAPGDEVDVVLEPEGPQRDDLAGDVATAL
                     DADPAAGEFFDGLAQFYRRAYLRWIDGATRRPDLRAARIDEVVALLHAGIKQRPR"
     gene            complement(22928..23791)
                     /locus_tag="PLZ72_02065"
     CDS             complement(22928..23791)
                     /locus_tag="PLZ72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HNA35111.1"
                     /translation="MSSLPSLLMGRCGANGDCGYQTCARYPGTWVAHPLAVPPKHPDH
                     CDERGGRNGDPTPDFEPGDHTRSSQGAEQRQTAAHTERGSTCSNGKQRIVPAARTSAV
                     CRQGFCLGHVVLSMFATTVSIDGNINPDPYSKVKEYCRRMRIGDLAAAAAIPTETVRY
                     YEKRGLLAAPPRSANGYRTYDDAALNQLRFIRTAQAAGLTLAEIGSVIELRDDGTTPC
                     GHVTALLADKIAEVRERRVQLAALEQELIRLVDRSATLNPADCRPDDICHVLSSDGSI
                     DASANWGPPLR"
     gene            23938..24243
                     /locus_tag="PLZ72_02070"
     CDS             23938..24243
                     /locus_tag="PLZ72_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35112.1"
                     /translation="MYHLDQITVDADAVIAQVAHKATDRPTPVCFCFAHTADDLVADA
                     ARNGGASTIKSAIKHAVADRRCACEHLNPSTKCCLADIHRSLTGTGPATTTDRVSPT"
     gene            24240..25601
                     /locus_tag="PLZ72_02075"
     CDS             24240..25601
                     /locus_tag="PLZ72_02075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA35113.1"
                     /translation="MSRTFDLYDLIVIGAGMAGSAAAEKCATAGWRVAIVDDLPYGGT
                     CALRGCDPKKILRRGAEIIDAAHLLTGKGIDPAGLAIDWTALSAHMHGFTDPVPANME
                     TSLGRHGVDTLHGTARFTGPDRLDIDGTNHQFRHALIATGARPRPLDFPGADHLIDST
                     GFLYLDALPPRILFVGGGFISFEFAHIAARTGTHTVIVDHGPRPLRGFDPDLVELLVT
                     RSHTIDIDVRRNTEIVAVHRSGDGYHVTLDDHRRQTTITTDLVVHGAGRQPELDRLDL
                     DAADIAHGTHGVHVASHLQSTTNPRIYAAGDAADTPGAPLTPVAVFEGRVAASNMLKA
                     AATTPNYSGIATAVFTIPELTRVGLLETEARDAGIDLDVRYTDTSGWYSNYRIGETAA
                     AAKILIDRATDTIVGAHMLGPEYGELINICALAIKLGLTTRHLKSMNAAYPTVGSDLG
                     SML"
     gene            25802..26968
                     /locus_tag="PLZ72_02080"
     CDS             25802..26968
                     /locus_tag="PLZ72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA35114.1"
                     /translation="MTEKRGVFYAVIYQGRNPVSGRDRRRWHRCDDRAGAEQLANDLT
                     EQRARQRHTGSSLTVAEYILGQWLPAKEATLAPTTHARYVTSVEHYLLPHLGDTPLRR
                     LRTEHLEALYRRLLITGSRRGGPLAAKTVTNLHQIIRSSLNDAVGRGLLASNPAQGSH
                     VPDPRKRPSTRRRARSWTAAELSDFLTETAANRYSMLFRLAAATGMRRGEVLGLRWDD
                     VHFDTRRIEVTQALTSIGYRLEFSRLKTRTSRRNITIDADTMDLLARWRQEQTTGLAE
                     AGAANAHGLVFVRPDGQPLHPHSVSQAFDRAQRPLDVSPIRFHDLRHTHASLLLRDRV
                     PIKVVSERLGHSNPAFTMTTYQHVLPGMQDDAAATFGQLLSNHSREVGEVAAAA"
     gene            complement(27097..28047)
                     /locus_tag="PLZ72_02085"
     CDS             complement(27097..28047)
                     /locus_tag="PLZ72_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35115.1"
                     /translation="MTAAESPQRRLFVAWRNPETRQIIPVGLLVQRRDGDAASYSFAY
                     LKLAETLDGFHPLPGLPDLHRRYDAPALFPMFANRLMPRDRADYPDYLGRLDLDVDAD
                     PFEVLGRSNGTRTTDRIEVFAAPERTPDDHAMSLFFVRGIRHLDGAPEAVDRLHDGQL
                     LAIVDDPDNVMNGRALLLAGDDRVPVGWVPDYLVEHLHELRQYNGADPTVTVEHINGP
                     DTAPHMRVLCRVTAPWPAGYEPFHSIEFQPVISLEEPTMGPNSATDPASERLPDLLPD
                     PVEAGGTGRDETAPPEPENPATPRGNGTRRHRPVQGSSNS"
     gene            complement(28044..28928)
                     /locus_tag="PLZ72_02090"
     CDS             complement(28044..28928)
                     /locus_tag="PLZ72_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNA35116.1"
                     /translation="MLLDVTAWRAGGEEQLGTKPKQWLRDPADRLWLWKAATWNPSPS
                     GEYRKGDDWAERVVTEIARSLDVPVATTELAERAGVFGTVSLSVLDPESERLVHGNEL
                     LAEIDVIGSDPHDRSGYSLDAVRRSLEGVAGSVPGSSAFECLAGYLIVDAVAGNTDRH
                     QENWAVIENPAAQRRLAPSFDHASSLGFLLDDAARSEMLHTSDRNQTPEAWAGRARTR
                     FEGRAHPVEIAAAAIDMIGPDVSGAYLEPLEQLDLRRIVARVPEFRISEPAREFASRV
                     AEANRARILSHLTRTMDP"
     gene            29177..30817
                     /locus_tag="PLZ72_02095"
     CDS             29177..30817
                     /locus_tag="PLZ72_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNA35117.1"
                     /translation="MSSNDNSRARSRSQLVASGVRVDRGVRRILTDLSLTVAPGTRLG
                     IVGENGRGKSTLLHVLAGRLTPDAGTVRRVGSIGIAEQEITVREDRTVDDLVDIELAD
                     TRAALRRLDDATRSLSENRAGADAAYAAALDHATALGAWDADRRVDVALGELGEITDR
                     GRRLTTMSVGERYRVRLACLLGASHDFLLLDEPTNHLDQHGLDFLTDALRATDAGVVL
                     VSHDRVLLKKVTTHILDLDPSIDERPRLYGNGYDGYIEGRRGELARWAQDYEQHQSEH
                     ARLAGDLSAAQNRLQSGWRPPKGTGKHQRATRAPALVRSVNRRLEDLRAHEVTRPQPP
                     LQFRMPDLPELAGVTLLRADNVTVTDRLKTPVSVELSSRDRLLITGPNGAGKSTLLAV
                     LAGKLAPSHGSTSVASRARLGFVGQESPSVDRRTARDIYRARLDQLLANGLTEADIVS
                     LRSLGLFTTTDLNRPVDELSMGQQRRLDLALALADRPHVLLLDEPTNHLSIALVDDLT
                     EALATTPAAVVIVTHDRQLRIDLSHWPTIDLGQERVTR"
     gene            30897..32036
                     /locus_tag="PLZ72_02100"
     CDS             30897..32036
                     /locus_tag="PLZ72_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA35118.1"
                     /translation="MAYIIKRKDRFYVVAYDGLDPLTGKERRRWHPAGRDRHEAEEIA
                     GRIQNDAAGAAPPRGGPVNLGEFLVDTWLPIKRRHVRATTAYRYSWMVDNYISPSIGH
                     IPLRRLHADHLDGLYDQLATNGGRAGTGLAAKTVHEVHMIIRSSLDLAIRRQLIGVNV
                     AQATNARHKRLARTVPRSWTAEELATFLAAAQPQRLYPALHLTALTGMRRGEVAGLKW
                     SDLDRANQRISISRTIQSIAGQPAEFPVKTRTSRRCVDLDEQTTDVLARWRRRLTRDG
                     LPHERDDWMFVNPAGRYVNPESISQLFDRLQRQLPDLSRIRFHDLRHTHASLLIMDGV
                     PVKVVSERLGHANVAFTMHTYQHLLPGMGATAARQFAALLTAHTR"
     gene            complement(32512..32997)
                     /locus_tag="PLZ72_02105"
     CDS             complement(32512..32997)
                     /locus_tag="PLZ72_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35119.1"
                     /translation="MTLRRFVWPWVHHKLEGAGPIIDDAVIIPNTPIDEVRAFVDTPV
                     KLAAWFGATFDADHGTLTIARAADALTVGWITTELIDHGRCLTLTGVIAPGPVRSYVT
                     LRSVAYQRTEADPDGAAVGFGTEVWTHVDLPLRTPPAVVRLVDDVMRSGNRHLRGELG
                     S"
     gene            complement(32994..34625)
                     /locus_tag="PLZ72_02110"
     CDS             complement(32994..34625)
                     /locus_tag="PLZ72_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA35120.1"
                     /translation="MTTQTSDPSGPVGDLPEGQHDPVEHDVVVENAGRRRLILAAMCV
                     ALVAVVASVSGLNVAQQALAQDLGATQSQLLWIINGYTLALAALLLPVGAIGDRWGRK
                     PILMIGLVAFAATNLASAFATTPGTLIGLRVLAGVAAAMVMPVTLSVITTSFPREEQA
                     KAIGTWAGFAGAGGIIGLFVSAAIIDNATWPWVFALPIALAAVSFALTAAFVPHSVEH
                     TEAGFDVVGSLLSVVAVGGLVLAFHEGPEQGWTAPITLAAIVAGIAATVAFVAVELRR
                     DHPLLDVRIFALRGLAAGSVNLFVVFAVMFSLFLVLVQYLQAILGYSALKAASGLLPM
                     AVMMMPLSTIAPTIAERFGFRRTLVTGMLAMATGLVVFAVLADPQGGYLTVLPGTLIL
                     GAGVGLAMSPSTAAITSSLPEEKQGVASSLNDTVREMGGAVGIALIGSVLNAAYQSNV
                     EPATAGLPPEVAHPVTEGIGGALAAAGQMGPNGATLIDAARQSFVDAMMPALLLAAAV
                     CAAAALFTAIRGPKTAAEAEPRLHTPEPQDNSGEP"
     gene            34723..35346
                     /locus_tag="PLZ72_02115"
     CDS             34723..35346
                     /locus_tag="PLZ72_02115"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35121.1"
                     /translation="MAPNPEIDPRVARTRRDVLAAAREVLLEDGWERVTLARVAERSG
                     YARTTLYRHWPQRLDLLRDLIGEEVRLLHTTPAGDLREDLIAEMEAFQAALTTSGLGR
                     MMIAIGQQARDDAELADLSSSVRADGSEVLRDIIAAGVERRELPSDLEPDVAVAQLVG
                     PVLFRFLFESEDDFSLASVTSVVDWFLAGTNAARGGGARVRSHGARR"
     gene            complement(35321..35761)
                     /locus_tag="PLZ72_02120"
     CDS             complement(35321..35761)
                     /locus_tag="PLZ72_02120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA35122.1"
                     /translation="MPAAITPDRYDADTAASMTAGSIDGLPSYLGIRTVEVGPATMLA
                     ELDVRPELLNPFGTLHGGVVSALVDHVLGAVLYPVIERGHWAATTEFKLNLLTAVRAG
                     TVSARSTIVSLTRRTAVVQVEVDNGGRPCALAQGTVLIVEPRGT"
     gene            complement(35776..>36490)
                     /locus_tag="PLZ72_02125"
     CDS             complement(35776..>36490)
                     /locus_tag="PLZ72_02125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA35123.1"
                     /translation="PPEPYPPQGTCTVNLDESPEELEARARMASALQRLGHSLVGHRV
                     DADLAGRIADLAAAATEEVSARPARDRTAEIATSPRFARWLGGDALEPIGLDGEPMDL
                     FRDSVVSGRTNPMGIGLEVRRRGDSVVATTTLGPAFEGAPGRAHGGVVAAIVDETIGY
                     VLPILGELAYTANLNLDYVAPAPLHQELRVTASLRDRADRKLWLQAHGESDDGIFVRA
                     EALFLTVDLTKFANDDSTT"
BASE COUNT         6041 a        12650 c        12310 g         5489 t
ORIGIN      
        1 gtgacctcga cgggagtggc agctcggcgc tcggcgagac ggagacggtc gacggcgccc
       61 tggtcgaccc gtcgggccca cccgcatgcg accaggtcgc ggagctcctc agcggttgct
      121 ccgcggcgta gcggtgtgcg caggttggtg ccggtggccg cgtacaggca atggtaccag
      181 aggccgtcgg cggtgacccg gctgcggtcg cacgaagcac agaacggggt gctggtcgaa
      241 gcgacgatcc cgaaggccgt cccgtcgccg gcccggtagc gccgtgccgg cgccgacggc
      301 cgtgtcggga gtgcggtcac cggcccgagc gcggcgccga ccgtggcgag gatctcgctg
      361 cgggacacga cgagccgctc gtcccatcgc gatgcaccgg cgacgtccat gtactcgatg
      421 aaccgcacct ccgcgcggtg tgccgcaccg aagtggacga ggtcagtgag ctcgtcgtcg
      481 ttcacaccgc gggtgacgac ggtgttgatc ttcgtcccgg tgaaccctgc atcggctgcg
      541 gcgtcgatcc cggcgatgac cgcgctgtgg ctgtcgcgct tggtgatctc gtggtgccgt
      601 tcggggcgaa gcgtgtcgag gctgatcgac aaacggcgca ggccagcgac ccgcaggtcc
      661 cgggcaaccg gtgcgagaag cgtgccgttc gtggtcatgg cgaggtcgcg gatgccgtcg
      721 acggtcgcga tcctcgacac gaggtccggg aggccgcgcc gcagcagggg ttcgcctccc
      781 gtcaggcgca ccttggcgac gccgaccgca gcgaagctgt gaaccaccct gggttcgttg
      841 gaggctcctg accttggaat cgtcccgttc gtcaggtgat caggaagttg cgcatcatgc
      901 cgccgtcctc gtgctcgagg atgtggcagt ggtagaggaa gtagccgcgg taggtgtcga
      961 agctcacggc gatccgcact cgttggcccg gccacaccag gacggtgtcg cgtgtgcccg
     1021 tctcgacgat gccgctggag atctgcgccc atgactcctc gcccgacgcg ttcacccatg
     1081 accggtcgac cacccggaac gccttgccgt gcaggtgcat cgggtgcgcc atcggggaca
     1141 ggttgacgaa ctcccagatc tccgtcgtcc cgaaggccac ggtctcccac tcggccgccg
     1201 tgcgcccttc ccactgcatg ccgttgatcc aatgacttgc ctgtcgggtc atgagctcga
     1261 agacccgcgg cgtgtcggtg ttgaccgcct cggacggctc gatcgtcacc tgaccggcca
     1321 gaccctgcgg aacctcgccc ggctcggcgg gaccgtcggc gacgacgaag ctcatggcca
     1381 ccttcgggtt gagtgtcaac gccgcaccac caccgatgcc tccacggccc atgccgccgt
     1441 tgcccttacc gccaccgccc atcattccgg ggacctcgac gaaggtgccg gcggtgagca
     1501 ggtcgatctg ctggcctggt tcgtacgagg agaagtcgat ccagagatca ctgcgctggg
     1561 cgggggtcag caccagcccg ggaacattcg cgacctcggg cagaagtccc ccatccgtgg
     1621 cgaccacctg gaggggctct ccggtcgaca aggtcaggat ctgggtccgg gagttcgccc
     1681 cgttgaggaa gcggagccgg tacggcgccc gctcaacgct cgtggcgaga ccggcaacgc
     1741 cgttggtcac cagcgtctct cccacgaacc ccgccatgac atcgtggttc gaggcgacgt
     1801 agacgagttg gccgtcggcg ccgatggtgc ggtcctgcag cacgaacccc aggtcccggg
     1861 caccgtgcgg caacgtggtc tcgggctcgt cgccgtggac gatcaggagt ccggccaggc
     1921 cccggtagac ctgctcgccg gtcaggtgat gagggtgggg gtgataccag taggtgccgg
     1981 gctcgttgac cacggtgaag tcgtactcgt aggtctcgcc ggggcccacc gcttcgaggg
     2041 gctgaccgtc ctgctcctgc gggacggtca acccgtgcca gtgcacgatg gtctcctgcg
     2101 acaactggtt ctcgaagatg acctgcaccc gctgcccggt gcggacgtgc agcgtcgggc
     2161 cgaggtacga cccgctcggg gtcacgctcg ccgggtcgcc gtcgatcacc tcggccccga
     2221 agctcaggac atcggtcggc gttcccgcga cgatctccgc cgtagccggg gccgccacca
     2281 gtcgaaggtg cagatcgcgg acctcggcct cgagctgagg catcccctcc ggcaccaccg
     2341 cggtcccggc cttcaccacg ctcgacgcgg tcgtggacgt cgacgaaccc ccgttgccgc
     2401 cgccgcaggc ggtgagcgcg acgagggctc ccgcgccacc gaccagaccg agggcggtac
     2461 gacgatcgat cggggtcgac gacgacgaca tgccctgggc ctgcggcgcc tgacgaccag
     2521 atgcgggacc ccgaccgcgt gagctctgac gtgccatggt tcgatccttt cagcgatcga
     2581 tcatccgcgc cgctcccttg gcctgatcgc gttccacgac tgcgcacgcg gtcgaggcgt
     2641 cagagtgccg tgatcgactc cagtggtgag cggcgggcgg cgacgagcgc cggcgcgatg
     2701 gtggcgatgg tgccggcgac ggcaccgagg agggcgatca cgacgagcct ggcgatcggg
     2761 atcaccggtg acgtgatggc ggcgttgtcg agcaaggtca cggtcgggaa cgagacgccg
     2821 atgccgatgg tcaaacccat cgcaacaccg aggatcgtcg tgatcgacgc ttccaggagc
     2881 accgatgaga cgacctgggc ccgggtcgtg ccgatggccc gggccactcc gatctcggcc
     2941 gttcgttccc ggatcgacag cgccatcgtg ttggccagcc cgacgagcgc gatcaccacc
     3001 gcgatgccga gcagggcata gatgaggttg aggatggtgt cgacaggtga cgagctcgcc
     3061 ccggcatact cctcagcggt ctggaaccga cctgacccgt tcgtcgctcg cgcgaccgcg
     3121 tcgagcgtgg cctgctgacc attggtggcc aggaagatcg cgaccgtccc ggggtcgctg
     3181 aacaccgccg agtaggtgtc ctgatccagg agcaccttcg gggaggcgaa cccagggagc
     3241 gccgtggcga acgatcccac cacggtgccc ttcgccacgc cggcgacgcc gcggatggtc
     3301 aggtccgagc cgacgggatg ggctcccagt tcggacgagg cgaccatcat ctgaccgggt
     3361 tcgaggtggc cggcatcggc cccggtggcg tcgaggtcgt agaccgcgtc gaggtcgtag
     3421 accgcgtcga ggtcgtagac cgcgtcgagg ttgtccgcct cgatcatgcc gacggggatc
     3481 gcgttcgggg tcgcggtcgt ggcttccgat gggtcggcaa ggaggccgcc cgtgacatgg
     3541 atggcggcaa gatcgttcac cccgggcacg gctcggagct cgggagtgag atcacccggg
     3601 accttcccgg cgggggcacc gaggggtgtg acgacatggt cggcgcgcag cgctgacgtg
     3661 ggaccgacct gaagggtctt gacggtcccg gcgaggatgc tgaagaacgc cacgacagcg
     3721 atgccgatca tcaagcccgc cgctgccgac gacacacgcc gcggcgatcg ggccgcattt
     3781 cgtgccgcga tgcgccccgt ggccccggcg acgacggcca ggaccggtcg tgccgcgcca
     3841 gcggccagcg aggcgaacaa cggaccggcc aggtagatcc cgatgaccgc caggagggtt
     3901 ccggcgagca tcagctggtt gcgctgcgat gcgatccccg cgctggtggc ggccacaccg
     3961 atgaccacga ggaccggcgc caagccgcgc cgccaccaag gggccgacga ctcgtccacg
     4021 gctgcatcgc ggagcgccgc ggcgggcggg acccgtgaac cagagacggc ggcccgcaac
     4081 gcagacagca acgtgatgac gacaccgatg aagatcgtcg ttgccagtgt tccagccgtg
     4141 aagatgaccg ggccgttgaa cgcctcgacg cccgctcgat cgaacagcag ttggatgagg
     4201 accaccacga accgaccgag cacgagaccc agggccgtgg cggcaacccc gaggatgaag
     4261 gcttcggtga gaacggatcg gacgagctcg gatcgggtgt tgccgatcgc tcgcatcagg
     4321 gcgagctcgc ggcgtcgctg caccatggag atggcgaagg tgttggtgat aatcagcatc
     4381 cccgtgagcg ccgcgacgat cgtgaacacc gtgagcaacc tcgacaagat cgtgctctcg
     4441 acggcgatcc gggccagctc ggcctcggcg gctgcatcgc cgcagcgcac ccgtaccgtc
     4501 tcaccggact gtgcgagatc ggcacgaagc ccgtcgacca gtacatccgg gctcacaccg
     4561 ggaacgccac gcaccacgat ctgcgtggcc acatcgccct gaccgatgag cgcaggctcg
     4621 tcaggggcca cgaggaccgt ggtccgcgaa gggagccgat cggcggggcc gtaccgggcg
     4681 atgccgacca cggtgagcgg cgtcgaggag cgttcggtgg cgagtcggta ctcgtcaccg
     4741 agcaccacac cgaagtcgcg ggcggtggcg gcatcgagcg ccatctcacc gggtttggcc
     4801 ggggcgcgac cctcagcgat gtgccaccgg gccgtctcgg aatcggtgat ccaggtctcg
     4861 gcctcgttga acggcccgcg gctctgcccg atgatcgacc cgtcggccga gaggagcgac
     4921 gcgttggcat gccagaccgc ttgcgcagcg gcaacgtcgg gatcggccgt gacggtgccg
     4981 aggacggacg tcgggatacc gatgccggcg gacaccatct ccgcaggacc ggagaaccct
     5041 gccgggggcg tgcccggaac cgcgtcgatg accgcgtcga tgccggcctc gccggcctcg
     5101 accgtggcct cgtccccggc gccgatcgaa tcggtgagcg tgagggtcgc gttcacgaag
     5161 gccacaccca tcacgatcgc gaggaaggtg agcaggtacc tgctccgact gatccagagc
     5221 tggcgcagaa cacggcgggt catcggtcgg ctccgagtga cttcatgcga tccagcaccc
     5281 cgtccgcggt cgggctggcg agctcaccga cgggagcgcc gtcctggaag aacaccacgt
     5341 tgtgggtgtg tgctgcggcg tacgggtcgt gggtgaccat cacgatcgtc tgaccgagat
     5401 ccgaggcctt ccgtaggtgc gacaagatct cgccggaggt gtgcgaatcc aacgaaccgg
     5461 tcggctcatc agcgaagacc acctccggct tcgacaccag ggccctggcg acagcgacgc
     5521 gttgctgctg tccgccactc agctgggacg gccggttgtc gaggcgatca cggaggccga
     5581 gcaccccgat gagctcgtcg aaccactggg ggtccactcg gctccgggac agatcgagcg
     5641 gatacgtgat gttctcccgg gcggtgaggg ccggaaggag gttgaaagat tggaagatga
     5701 acccgaggcg agtacgccgc aggatcgtga ggtcgcggtc gctgagatcg cgcagatcga
     5761 cgccaccgat ctcgaccgtg cccgacgtga gccggtcgag tccggccatg cagttcaaca
     5821 gcgtcgactt tcccgagccc gacggcccca tgaccgcagt gaactgacct cggggaaagg
     5881 cgatggtgac gtcgtcgagc gcccggactt cggcgtcggc ggagccgtag accttcgttg
     5941 ctgccgtgag ggcaaccgat ggtgtttcgt cggtcatcac gtcctccttc tgagacatcc
     6001 agtctcgcta tgagacatac tgtatcagaa gaacacaccg atacactctg tctcatgtca
     6061 ccgcccggcg agacccagcc cgatcctcgg atcacagcga cccgcagccg ggtgctcgct
     6121 gcggtctcgt ccctcctcga gtcgggcggt ccgagcgagg tcacccactc ctccgtggcc
     6181 cgggcggcca aggtcggacg cgccaccgtc taccggcact ggccccggat cgacgacctg
     6241 ctggccgatg cgatcgagac gcgcctggga gtgactccgc ccggaacgac cggcaacgcg
     6301 ctcaccgacc tccggtcgat gctcctggcg atcgtcgctg gcgtgttctc cgacgacagc
     6361 gccgtgttct tcacgacgct cctggtccag ggaggcgaca ccccgcagat ggatgccctg
     6421 cggcgagaga tcatcgagcg ccggatgggc gcgatccgcg acctggtcga acgcggtgcc
     6481 atcgacggat cgatcgaccc gaccctcgac cccgaccggg ccatctccta tctggccggc
     6541 ccgatcatgc atcgacgcat cgtcctcggt cagccggtca ccccggactt catcgacgac
     6601 ctgctcctcc gagtcgccgc cacgccgccc tgaccaaaca ccggttgcga cggtgtcatg
     6661 tccaggcgtc gctgggcgag gcgtgcaaga ccttcgggga cgtcgtggag gatcaacccg
     6721 aaggcgatca ggaagacacg acacagctct tcggcccgca gcccggagtc ctcaggggtg
     6781 cgagggaggg atggccgcgt gcaacaccgc caagagcccc gcaccccagg cgcgcaccag
     6841 ccatgtcgag gcgtcgatgg cttcgatcaa cgctcgttac cacaacgatc accggtggtc
     6901 tcggcagggc gcacagcgct cggcatctgc cgaccagcgt ccggtacctt cgtcccacgc
     6961 gattccgtcc gccggattcg acgaggaccc ggtctgcgaa gcggggccga atgcccggtg
     7021 gaatacccca tggggtatat gtgttgcagg acacaccaca cgtcgaccgc cgactgctga
     7081 tcaacagatg gagccaacca tgcccgcttt gcacctcggg gacgaagccc ccgatttcac
     7141 cgccatgacc accgatggcg agatctcgtt ccacgactgg aagaagggct cgtgggcggt
     7201 gttcttcagc cacccggccg acttcacccc ggtgtgcacc accgagctcg gtcgcaccgc
     7261 tgcgctgcag agcgagttcg ccaagcgcaa caccaaggcc atggccctgt ccatcgaccc
     7321 catcgaggag caccacaagt gggcccccga catcggcgag gtcaccggaa cccccctcaa
     7381 cttcccgatc gtggccgacc ccgaccaccg catcgccgag ctctacgaca tgatccaccc
     7441 cggcgagggc gacacctcca ccgtgcggtc cgtgttcatc gtcgaccccg acaacaagct
     7501 ccgcctcatg ctcacctacc ccaagtcggt aggccgcaac ttcgacgaga tcgtccgcgt
     7561 catcgacgcc ctccaggcca ccgacgccaa ccccatcgcc acccccgccg actggcgccc
     7621 cggcgaccgc gtcatcgtcg ccatggccgt gtccaccgaa gacgccaaga agcagttcga
     7681 caacgtcgag gagttcaagc cctacctccg ctacgccgac gccccccagt agtccgagga
     7741 cggtcagagg cagatccccg tggcggtgcc gcagggttca tgagcgactg gcggacgggc
     7801 aacccttcgc cggcggagcg accaagccgg tccgctccgc cggtggaggg ctcgaacgcg
     7861 tggaagagcg gttgccgcga tggagtgcgc ccgctcatca gtgagcgggg tcgccccttg
     7921 tcctagcggg acctcgagac gggggggtgt cgacaaccgt cccgctcgac gaacgttcta
     7981 ccgattgttc ctccgggtcc cgatggccgg gatctacacc atcacggagg aacatcatga
     8041 acggatacgt ggcacaacgc cggggtcgct tctacgcggt catctacgaa ggccgcgacc
     8101 cggtcaccgg caaggagatc cgccgctggc acccggccgg taccgatcgg gccgaggcgg
     8161 agaagctcgc cgcgaagctc gccgccgccg agaccaagcg ggtgggcgcg gtgcggtcgt
     8221 tgaccttcgg cgcgtacctg accagccagt ggctgccggc gaagaggctg catctcgcga
     8281 ccagcaccta tcggggctat gagcgcaacg tgcagcgcca catcctcccg gtgctcggcc
     8341 ggaccagcat ccggcggctc cggtaccagc agatcgaatc gctctacgag gcgctgctcc
     8401 atccggagac gggccgaggt ctcgcaccca agaccgtcta tgagatccat ctcgtcatcc
     8461 gcgtcgcgct cgccgacgcg caccgccgag ggctcgtgac ccgcaacgtc gcgctcgtgg
     8521 cccgcgcccc aaaacaacgg tcgctgcaac ggatcgaagg tgtctcgctg acggatgagg
     8581 agctccgcca gctgttgcgc accgcggcgg gccaccggtt cttcccgatc tactggctca
     8641 ccgccatgac cgggatgcgc cgcaacgaag tcctcgggct caagtggtcc gacctcgacg
     8701 ccaccaagaa gcggctgcac ctcaaccgcg ggctcgtcgc cgtcggctac gaggtccacc
     8761 agacccgcgg caagaccaag accgcccgcc gcgtcatcga gctcgacgcc accaccatcg
     8821 ccgtgctcga aggctggcgg gcctaccagg ccgccgagtt cgccgcggtc ggcatcagcg
     8881 agagcgacga ctgggtgttc accgacggcg acggtgaacc ggtccatccc cacgccgtgt
     8941 acgaggcgtt ccggcggatc gtccacaacg ccggcgtccc cacgatgcgg ttccacgacc
     9001 tgcgccacac ccacggcagc ctgctcatca aggaaggagt cccggtcaag gtcgtctccg
     9061 agcggctcgg ccacgcgcac atcgcccaca cgctcgagac ctaccagcac gtcctgcccg
     9121 gcatgcagga agacgccgcc cgaaccgccg aacgtctcgc cgacccacgc ccgaccgcga
     9181 agaagccgaa gaatcctacg aagcggcccg gcaagacgcc ggtggaacgt cgtgggaaca
     9241 accggaggaa ggccgcctga tccgcaggaa cgtcccctga actgcgaaag cccaggtcgc
     9301 tgacctgggc tttcgtgctt cagatggtgg cgggggcagg atttgaacct gcgaccttcg
     9361 ggttatgagg cgacgatcag ctcgtcagcc cgtaccaccg tgtaccggat cgtgccctct
     9421 tgcttgggtt tcgagttcca tcgtaccgcc gacgtgccag cccgtacacc cctgtaccgc
     9481 gccgcacggt agaaggaaca gtagaagcgc ccgggtgtcg atgcgggttc gtcgagtgac
     9541 ggatcgaggc gtcagcttcg cgttccggcc ctcatcggct ctgcccccga agctgtcgac
     9601 gacgccggca agccgacctt cgggttcgct cctcgagacc gagagcgcga ccccgctcga
     9661 cgcttcgttg tggaccgaac acccctcccc tcgctggcgc ggagccgcat cgggacgacc
     9721 cgaccagcgg gagaccggat gcgttgccga ttcaagccgc atccccggga tcgccatggc
     9781 cgcgctgatg atcgggctcg ttggaacgca gggagctgaa cgcgaggagc atcgcaccgc
     9841 agacgatggc gacatctgcg aggttgaagg tgggccacca tccggtgtgc agcatgtcga
     9901 ccacggtgcc ggcctcggcg cggtcgatga cgttgccgat cgcccctccc acgatgagtc
     9961 ctgccgccca cgggttgggg agccagcccc gccacgcgcc gatggcgatg agcgcggcga
    10021 cggtcgtggt gacggcgagg acgactgcca gcgggaggtt cgatccgaga ccgaacgctg
    10081 cgccggggtt gtaggccagt tgcaggtcga gcggaccggg aagctccacg ggagcgtcgc
    10141 gcagccaggt ggaagccgcc accttcgagg tgacgtcgac gaccagcacg gccagcgccg
    10201 taaaggcgag caccgtccgg tcgcgtcgac ggtcggggga gccgacgcgc ttgggtgtcg
    10261 gtgcggaggc cgacccgcgt ggattcgacg gcggacccgt cactttcgtg ccattcgggt
    10321 gccgttgagg atgacgacga gttcggacag ttcgtgggcg agcacggcag cggggagtgc
    10381 gatgaccccg aacagggcga gtggtacgag cgtggcaagg atcacgaggg agagggcaag
    10441 gttctgggtg acgacccggc gggtgtggcg accgatctcg atggcagtga cgagcttcga
    10501 gaggtcgtcg gccatgaggg cgacatctgc ggtctcgagc gcgatgtcgc taccggcggt
    10561 acccatggcg accccgaccg acgccgcagc gaggggcggc gcgtcgttga cgccgtcgcc
    10621 gaccatcgcg acgtgtccgt actgctcgac gaggcgttgg acgtgtgccg ctttggcttc
    10681 cggtgtcatg ccggcggcga cgtcgtcgat gcccacctgt gcggcgatcg cggctgcggt
    10741 gcgagggttg tcgcccgtga gcatcacgac gtggtcgatg ccgaggccgc ggagtcgggt
    10801 gatcgtgtcg gcggattgtt cgcgcaccgt gtcggccacc ccgagcactg cgaccggtcc
    10861 ttggtcgtcg acgaggacca cggtggtgtt cccggccact tccatctcct cgatcatgtc
    10921 ggcgatcggg ccgaggtcgt ggccctcgtc ggtgatgaaa cgtgggctgc cgaccgtgta
    10981 ctgcttgccc ttgatgcgtg cgtgcacacc gccgccgacg gcggcacgga agtcctcggg
    11041 ctcggaaaag tcgaggccct tcgcttcggc ggcgtcgacg atcgcggtgg cgatggggtg
    11101 ctcggagcgg cgctctgctg atgcggcgat cgccagagcg cgatcggcgt cgaggtcgcg
    11161 gagggggtgc agttcggtga gctgcggttt gccgcgggtg agggtgccgg tcttgtcgag
    11221 ggcgacggcg cgtagccggc cgagttcctc gaggtagatg ccgcccttga tgagtatccc
    11281 ggagcggctg gatcggccca tcgcggcgac gtaggtgacc ggaatcgaga tgaccagggc
    11341 acagggggcg gcggcgacga ggaccgtggc ggcgcgcagc gcccagtcgt tccacgaccc
    11401 ggtcaccgcg ccaccggtga tccacacccc ggcagcgagc gccaggaccg ctggggagta
    11461 caccttcgag aagcgctcca tgaactgctg gccgcggccc ttgcgttcct gggcttcgct
    11521 gacgagctcg acgacccggg cgagggtgtt cgtcgccgac gttgcggtcg cctcgacgac
    11581 gagcgcgccc tgcgcgttcg aggtgccggc gaagaccagt tcaccgggca tcttgtcgac
    11641 cggcaccgac tcaccggtga cggcggcctc gtcgaccgac gaggtccctt cgaggaccct
    11701 gccatcagtg gcgatgttct ggccggggcg caccaggaac cggtcaccga cgaccacctc
    11761 gtcgacatcg acctcgcgtt cggacccgtc cgctccgagc agagtgacgc ggcgcggcgc
    11821 gaggtccaac agcttgcgga ccgcgtcgcg tgtgcggtcc tcggtgaact cctcgagcga
    11881 ttccgagatc gagtacaaga acgccagcgc cgcggcctcg gaccacgcgc cgagcacacc
    11941 ggcgacgacc gcggcgacgg tcatcagcaa ctcgatgccg atcgcacgct cccgtacgag
    12001 ctcctcgacg gcttcggcgg cgaagaagcg ggcaccgacg agcgccgcgg cgatgaacag
    12061 cgcggtcgag atcgcgtcga cagctccgag gggtccggcc agatagcccg ccgcgatcag
    12121 cagaccgctg aacccggacc agcgcacctc gcgggagcgc cacaggtcgc cgagcaggcc
    12181 gcccccctcg ccctcggtcc gggtcggcgc gacaggggtc ggtgtcgcgc tcatcgcttc
    12241 gtccgatagt tctcgcacag ctcgacccgc tcaccgatct cgcccagcaa cgcctcggcc
    12301 tgacgcaaca ggtcgacaac cgcgggggtg gcgagccggt agtggaccga gcggccctcc
    12361 gggcgatccg agatcaatcc gcactccttc aggcacgcga ggtgcccgga gacgttcgac
    12421 tgcgaacagc cgagcacgtc gaccaggtcc acgacccggc gctcaccgcg gagcagctcg
    12481 gtgaggatcg cgagacgagt cgggtcgccg aagctgcgga acatgtgcgc tgcgatcgca
    12541 atgtctggtt gcacggtggt tcccatcagg acaactccct tttcatcggc atgaaccgat
    12601 gttagcgttg atggccgatg gagtcacgca cggctccgcg aaacgatgga gatcgatcat
    12661 gccggtgatg tcgagagtcg agcgggcgtt ctgtcgcagc gcaccgtggc agagcttcac
    12721 cggtcgggtc gtgctgccgt ggctactggg cgacaccgag ttggacggcc aagtgctcga
    12781 gatcggctcc ggtcccggag cgaacggccg ggcactcctc gagcgatacc gggccgttga
    12841 actgactgca acggacctcg atccggtgat ggtcgccgca gcacgccgac gtctcgcccc
    12901 gcacggcgcc cgcgtcaccg tgaccaaagc cgacgcaacc gacttgccgt tcgacgacga
    12961 tcacttcgac accgcggtga gcctgctgat gctgcaccac gtcatcgact ggcgcgacgc
    13021 cctggtggag gtcgccaggg tcctccgccc cggcggcacc ctggtcggct atgacctcac
    13081 cgaccgccgg accgcgcgca tcgtgcacct cgtagaccgt tctccgcacc gactgctcgc
    13141 atccggagaa ctcgcagacg ggctcgacgc cgccgggttc accgccgtcc agatcgacac
    13201 cgcagccctc ggcaccgtca ccagattccg cgctgaatga acaacacctg gtgtcgtgtg
    13261 gatggaccgt gtggggcatc gcgcgatcag gcacggcaca gcgtgcgtcg ggccagcccg
    13321 cgatgagcac cgatcgcacg atcgccttcg tggacctcgc cgggttcacg gctctcaccg
    13381 agacccacgg cgatctcgcg gccgtcgatc tgatcgacat gttcaccggc atcgcaacca
    13441 ccgccatcga gggcaccggc accgagctgg tcaagacgat cggcgacgcc gtcatgctcg
    13501 ccgccgccac ccccgacagt gccatcatcg cggtacagcg actgttcgaa gcggcctacg
    13561 acctcggcgg gttccccgaa ccccgtgcag ggctccacca cggatccgtc gtcgctcgcg
    13621 gcgacgactt cttcggcgcc accgtcaacc tcgcagccag agtcgcgagc cgtgccggtt
    13681 ccggtcaagc cctcgtcacc aactccatgc tcagcgccgc ccacgacgcg gacatcgaca
    13741 cggtcagcct cggcctgcac caactccgca acatcctcga gcccgtcgag ctgtgggcac
    13801 tcgagctgtg ccccacccac atcgacctgt ccgtcgaccc cgtgtgccgt atgcgtctcg
    13861 catgccaagc cgcgatcggc cgggtccgcc atcacggtgt cgagtaccgc ttctgctctc
    13921 tcccctgcac cgccgctttc gccgccgacc ccgaccgcta cctcgacagg ggagcctcgt
    13981 gaccacgagc accgcgcccg aaccgccgac tgacagtgac agagggaaca ccacggtgcc
    14041 caccccccaa caggaccaca cgcgaacccc accgccctcc cccacgctcg gcgccgacac
    14101 gcgaccctga tcgtcgccgc cgtcgcagtc ctcgccatgg acatccgacc aaggtcgcat
    14161 cgagcgaatg gctcaccggt cgcggcgtcg tcgaactccc cggcccgctc gacctccagc
    14221 tcggctacaa cccggcatcg cattcgacct cggtgacacg gtgccccagt gggtcggccg
    14281 tcacagcgtt gcgaacggtg cgactccgtc gtcgttgaac gagaggactt cgaggggtct
    14341 gccatcgggc tcgcccatcc caggggcgtt ggtgggcatg ccggggatgc cgatgccgtc
    14401 cacggcgggt cgctccgcca ggagcttgtc gatcgcttcg acgggaacgt ggccctcgac
    14461 ggcgtagccg tcgaggagca ccgtgtggca actcgccgcc tcggacggga cgccgttctg
    14521 cgaacgaatg ggtgtcaggt cgtcacggac ttcggactgg acgtcgaagt cgtgcttgcg
    14581 gaggtaatcc tcgtagctct tgcagcagcc gcaggtcggg gttcggtaga cgaccaccga
    14641 cggacgagcc gccgagatgg atggcgaggc ggacgagcgc tcgaccggtt cggtcgatcc
    14701 gcatccggcg agagatgctg ctccgagaag gacgactgcg gcgcccccta cgcgaaagga
    14761 gccgcggcgg cttctcggtc gagcgagaat gctaaatcca cgacgatcgg acacggtcag
    14821 aacgctaccc ggtgctagtg ccgagctgag atcggcgttg gtggtaaagg acacgaaact
    14881 ggtcgatccg cactcgggac ggcaccggtc gaacgcccga cgcgagaaca ggtcgcctgc
    14941 cagcgctgat ctgtcctgcg atggcggaga tcccggcacg ggatcatcac ccccgatgcc
    15001 gagcgacacc gccccggcca tgctcaggct gttcgcgtct cgtaggaacc cccgcctgct
    15061 cccattctcc gaatgggcat tcacgtcagt cgagcgacgc cgacgtcact tcttcttcgg
    15121 cgatcgggtc gagttgaagg tcgacccggc gcagcagctg ggcgttgagg gcgacgacga
    15181 tggtcgacaa gctcatcagg atggcgccga cagcagggga caggtcgagt ccggcccacg
    15241 cgaacacgcc ggcggcgagc gggatggcga cgatgttgta gcccgctgcc cacgccaggt
    15301 tctggaccat cttgcggtag ctcgcccgcg acagccggac caccccggtc accgaacgcg
    15361 gatcgctcga agccagcacc accccggccg attcgatcgc gacgtcggta ccggcaccga
    15421 tcgcgagccc gacatcggct ctggcgagcg ccggagcgtc attgacgccg tcacccacca
    15481 tcgccaccga cagaccctgg gcctgcagct cgctgacggc acggtccttg tcctcgggca
    15541 gcacctcggc catcacccgg tcgaggccca gctcggcgcc gaccgcgtca gcgacctgtt
    15601 gggcgtcccc ggtgatcatc acgacctgga tgcccatcgc cttcaactcg tcgacggcct
    15661 ggcgggcctc gggacggacc tcgtcctcga gcgcgaacgc cccgatgatc ccgtcaccgt
    15721 ggaacaggta gagcgcggcc gctccacgcg cgacccactc gtccgtgcgt cgctggacct
    15781 cgcccggaac gggtgcgccc aactcacgga gcaacgccag tccaccaacg gagtactgct
    15841 cgccgtcgac cacggcatcg acccctcgac cggttcgtgc ccggaagtcc gtcgcagcag
    15901 gaacgtcgcc ccggcgagcg gcttcggcgg tgatggcccg ggccaacgga tgctcgctgt
    15961 cgctctcgac cgcaccggcg atggcgacca tccggtcctc gtcgaccgtg tcggccgccg
    16021 cgacgccggt gaccacgtgg gcgcccttgg tgagtgtgcc ggtcttgtcg aacaggacgg
    16081 cgtcgatcgt gcgcatgcgc tcgagcgcca ggcggtcctt gaccaggatg ccggcccggg
    16141 ccgacaccgc ggtcgagatg gcgataacta gcggaatcgc cagtccgagc gcatgcgggc
    16201 aggcgatcac cagcaccgtc acggtcttca ccacagccga atccacgtcg ccgagggccg
    16261 cccacgcaac gaacgtcgcg agcgctgcac cagccgcgac gtagaagagc agcgcagcga
    16321 atcgatccgc caacacctgt gcccggctgc tggactcctg ggcatcggcg accatccgct
    16381 ggatgccggc cagtgcggtg tcatcaccga ccgctacgac gctgatcctc agcgacgagt
    16441 cggtcgagac ggtgccggcc acgacgcggt cgccctcacg cttctcgacc ggtcgcgact
    16501 ccccggtgat catcgactca tccagctcgg cctcgccatc gaggatctcg ccgtcgaccg
    16561 ggacacgacc gccggcacga acgagcacga cgtcgcccac ggccagcgtg tcgatcggca
    16621 cggtctcggt gacgccctcg ccccggacga cctcggcctc gtccgggatc aactcggcca
    16681 gcgcagcgag cgcgttctgc gcctggccga tcgccttcat ctcctgccag tgacccagca
    16741 acatgatcgt caccagcgcg gcgagctccc accagaagtc caggtcgaac cagccgagac
    16801 tcgtggccat cgacgccgcg tacgcaacgg tgatcgccat cgagatcagc aacatcatcc
    16861 cgggctgacg gtcgcggatc tcgcgcactc cgccctggag gaagggccag ccggcccaca
    16921 ggaacacgat cgagccaagc accgggccga cccattcgat gcctgggaag tcgagcgtgt
    16981 agccgaacca gtccatgacc atgtggctcg tcaccacgag ggggacggtc aggatcagcg
    17041 agatccagaa gcggcgccgg aacatctccg ggtcgtggcc tgcgtgcttg ttgtgccccg
    17101 cgtggtcatc gtgcccggaa tgctggtggt gaccggtgtg ctgcgctgtg tgtggttcat
    17161 gcggcgtcga cgacatggtg acccccttcg gaccgtatct ctggagtgcg gccagctgcg
    17221 ccggcggagg cggacagcgg agcgaaacgt cgcagccgga ggctgttggt gatgacgaag
    17281 acggaggaga acgccatggc ggcgccggcg atcatcgggt tcaggtaccc ggcggcagcg
    17341 agtggaaggg cggcgacgtt gtaggcgaac gcccagaaca ggttgccctt gatcgtggcg
    17401 agcgtccgtc gggcgaggcg gatcgcgtcg ggcgctgccc ggaggtctcc gcggacgagg
    17461 gtgaggtcgc tggcctcgat ggcggcgtcg gtgcccgtgc ccatcgacag tccgaggtcg
    17521 gcctgggcga gggcggcagc gtcgttgacg ccgtcgccga ccatcgcgac gaccttgccg
    17581 tcggcctgga gacgctggac gacgtcgacc ttgtcggagg gcagcacctc ggcgatgacc
    17641 tcggtgatgc cgacctgggc ggcgacgctg cgggcggcgg cggtgttgtc gccggtgagg
    17701 aggatcgggg tgagtccgag gtcgcgcaac tgctggatcg cttcggccga ggtcgccttc
    17761 acggtgtctg cgaccacgaa cagcgcccgg acctggccgt cccacgcagc gaggaccgcg
    17821 gtccgggcct gcgcttcagc ctcggccttc tcggcgagca gtgtctcgct gggggtgagg
    17881 ccccagtcgg cgagcaacgc cgggcgacca gcgatcacac cgtgcccctc gacgacaccc
    17941 tcgacgccga ggccttcgcg gttggcgaac gactcgacgg gcggcagttc gccgacctcg
    18001 tcggcggcgc cgttggcgat cgcctgggcg atggggtgct ctgaggcgtg ctctagtgcg
    18061 cccacgatcc gcagcgcgtc ctccctggtc gtgccgggct cgacgacgac gtcgacgaga
    18121 gtcatcttgc cggtggtgac cgtgccggtc ttgtcgagca cgacggtgtc gaccttgcgg
    18181 gtcgattcga gcacctcggg gcccttgatc aggatgccga gctgggcgcc gcggcctgtg
    18241 cccaccatca gtgcgacggg ggtggcgagt ccgagggcgc acgggcaggc gatgatcagc
    18301 acggcgacgg cggcggtgaa cgccgccgtg gttccgccgc cggcgccgat ccagaagccg
    18361 agcgttgcga gcgccagaac gatgacgatc ggcacgaaca ctgcggagac acggtcggcg
    18421 agtcgttgca cctcggcctt accggtctgg gcctgggtca cgaggcgggc catctgggcg
    18481 agttgggtgt cggctccgac gcgggtcgct cggatcacca gccgcccgcc ggcgttcacc
    18541 gtggcgccgg tgacgtggtc accgggcacg acttcgatcg gcacgctctc gccggtcagc
    18601 agcgacgcat cgactgcgga ggagccttcg tcgacgatgc cgtcggtggc gatcttctcg
    18661 ccgggccggg cgacgaaccg gtcgccgacc tggagctggt cgatcccgat gcgttcctcg
    18721 cggccgtcgc ggagcaccac gacgtccttg gcgccgaggt cgagcaacgc ccgcagcgca
    18781 gcaccggtgc ggcgcttgga cttcgcttcg aagtagcgcc cggcgaggat gaacgtggtg
    18841 accgctgccg cgacctcgag gtagatctca tctgcgccgg agccgcgttg gatggtgagc
    18901 tcgaagggca tcgtcatgcc gaccacgccg gcgtcgccga tgaacaggct gtagatcgac
    18961 catccgaacg cggcgagtgt gccgatcgag atgagcgtgt ccatcgtcgc tgtgccgtga
    19021 cgcaggttca gccacgcggc ccggtggaac ggcaacgcac cccacacgac gactggcgca
    19081 gcgagcgtga gggatagcca ctgccagttg tcgaactgca gcgccgggat catcgccatc
    19141 gcgacgaccg ggatggtcag cgcgagcgag acgagcaagc gggtgcgcag cgcagcggtc
    19201 ggatcgatcg ttgcagcacc ctcgttgtca tcaccttcgc cacccggcgc ctggggcagc
    19261 tgggcggtgt aaccagccgc ctcgacctcc gcgacgagct cggcagggtc gacgtccacg
    19321 tcgccgtcga tcgtgaccgc cgcacgttcg gtggcgtagt tcacggtggc ggtgacgccg
    19381 tcgaccttgt tgagccgctt ctcgatgcgg ttggcgcacg acgcgcaggt catgccgctg
    19441 atggtcaggt cgagcgtgcg aaccgcggag tcgggagtgc cctcgtcggt gcggtgggtg
    19501 gtagcggtca tcgtcctgct ccgttgctgt ggtggccgtc gctgtcgtcg tgcgacgcgg
    19561 acgtcggggt ggtcgtgctg ctgtcgggcg ctgctgctgc gggtgcgtcc gtgctggcgg
    19621 ttgccgtgaa ctcggcggtt ctgaccacgc cgtcgtgaag gaagtccagg tagagccggt
    19681 aggtccccgc cgaggggacg ctggcggcga actcgatggt cggccccgcc gtcgtcgtgc
    19741 cgtcgccggg ttcaccctcc gggtggacgt gcagataggc gacatcgcct gagcggagcg
    19801 cgacgaggtg gccgtaggcg cccaggtacg gctccagatt cgtgacgggt cgaccatcct
    19861 tcgcgaccga cggggtcagc gccgacgacg atccgggcac caggtcacct gtgagggtga
    19921 cggtgtagcc gtcgatctcg gcggtcctcg acggcgcagg cagtggcgtg gcgcgctgct
    19981 cgcccgggac ctggaggtcc aggccgagcg tgagcccgtc gccggacgct gttggggtga
    20041 agtcggcgag gatccggtag gtgcccggga cggacaggtc agtggttgtg ctccagacgc
    20101 cctccgcgtc acgggtggga tgcagatgct ggtaacccga caggtcgcgg cgcaccacga
    20161 tgaggtgcag ctccttgtcg tgctccttgg tgtactcgtt caccgggagg ccgtcggggc
    20221 cttcgatcgt gaatcggtag tcggcggtgt cggtggcctc gagcgccgtc tctgtcggga
    20281 cgagggtata gccggcctgc gaggtctcaa ggcccccgag cgtggtggcg gtgtggtcca
    20341 tggcgaccat ctcgtcgcct cctgccgtgt catgggcccc gtcgatcgac ggggtgtggg
    20401 tagtgggctg attctgggtg tcgaccgggc cgacggccgc cccgacgcca agggcagcga
    20461 agaacaccgc tgccgacagg gcgccgaagg cggcgagctt cgtcgggacg ttcatggcct
    20521 gacctcgtac ccggcctcgt cgatcgccgc gttcacgtcc gcctcttcga ggggggcgtc
    20581 gctggtgatc tgcaccgtgc ccgcagccag gtcgacgtcg accccggtca cgccgtcgac
    20641 cttgcggacc tcgttcgtca ccgaggtgac gcagtgtccg cagctcatgc cgtcgacgtt
    20701 gattttcctg gtggtggaca tcggtgactc cttcgtggtc gataccccta tggggtatgt
    20761 ctgacgatac ccctatgggg tatactcgtc aacaatcgat ccacgagatg tcgtgctgga
    20821 ggtaccgatg accgaacgcg gatacaccgc aaccaaggaa cagctgcagg cccggctgtc
    20881 acgcatcgaa ggccaggtcc gtggcgtgaa gggcatggtc gacgacgacc gctactgcat
    20941 cgacgtcctc acccagatca gcgcgatccg ctccgcgctc gacaaggtcg ccctcgggct
    21001 cctggacggg cacacccggc actgcctcgc tgacggacac ggcggaccca ccgatcctga
    21061 cgaacaggcc gacgaactga tgagcgccgt cggacgcctc ctcacgcgat gagccgcccg
    21121 gcaatctgcg cggtccgtcg agagcacgcc cgacgtcgga cggacaactg cggcgacgga
    21181 ccgtcgtcac ggacgagtcg tagggtgacc gccatgacac gacgggcaac ggcggtcgca
    21241 gcgatgttgc tgctcggatt cggcgtgctc ggaatgcacg tgatgctggc cgcaccctcg
    21301 atgtcgcccg accaatccgt cggtcaggaa tgtcaccatg accaggactg ctcaccgtca
    21361 ccgatgtcgc acctcggagc gatgtgcgca gcgatcctcg ccgtcatcgc cgttgccctc
    21421 gtgggattcc gacggcgagg acgatcagtc cttcggcgcc tcgtcgctcc gagacggatg
    21481 cgaccgggac cgacggcggc acccgctgcg ccgaacctcg cgtcgttgtg catcctccgc
    21541 tgctgagcgg cctctgtggg accgggcccg ttcccggacg ccaccactag agaccgaaca
    21601 atgaggagac cacatgacat cccgacagat ccgtcgcgca ttcagcgtag cgatcctggc
    21661 cgtcgcgctc gtcacgaccg cggcggcgtg cagcaacgac gacggcacca ccggcaactc
    21721 aggagccacc acgacccggg ggcagacggc tacggctgaa cttcccgacg acgtgaacga
    21781 cgccgacgtc gccttcgtac aaggcatggt cccgcaccac gaacaagcca tccagatgtc
    21841 cgaactggtg attgccaacg gtgacgatcc gaccgtgatc gccctcgcgg agcagatcaa
    21901 cgccgcccaa ggacccgaga tcgaccagat gaacacctgg ctcgacgact gggacatcga
    21961 cccggccgga tccgacggca tggacggcat ggacggagcc aacggcatga tgtccgacga
    22021 cgagatggac atgatgtccg aggccatggg cgccgatctc gaccgaatgt tcctcgagac
    22081 gatgatccga caccaccaag gggcgatccg gatggcggag gtcgaactcg aggagggctc
    22141 gaacgccgac gtcctcgcgc tcgcccaagg gatcatcgac ggccaacagg ccgagatcga
    22201 ccagatgcag tcgatgctcg aagacacctg acccattcgt gcggggcggt caacggcgac
    22261 cgccccgcac tccgtgctcg cccggcggcc tgcacgggga accaatctca gcgcggtcgc
    22321 tgcttgatgc cagcgtgcag cagtgccacg acctcgtcga tgcgagcagc acgtaggtcc
    22381 ggtcgacgcg ttgcgccgtc gatccagcgc aggtaagcgc ggcgatagaa ctgggcgagc
    22441 ccgtcgaaga actccccggc agccggatcc gcgtcgagcg ctgtggccac gtccccagcg
    22501 aggtcgtcgc gctgaggccc ttcgggctcg agcacgacgt cgacctcatc gcccggcgcg
    22561 atgccgcagt cccgccgcca ggccggcccg agcaggatcg ccgatcgctc gtcgagtgtc
    22621 tcgatgacgg cgcgcaccgc catgccgttg acggtgccag cgacagggtg ggcgggcttg
    22681 cggccccatg cgtcgtcggg atcgaagggg agcgggacca ggacccgacg cctcgggtcg
    22741 cgcacgacgg ttgtggtgaa tcgctgcgtc cggtcatcca tcggtctgat tctcgcgctc
    22801 gtcggtcgat cgaaccgcac ggcttcggct ggtcgtcagg gcgttcaccg gccgagcgac
    22861 tgacgttccc tccgatgaca ctgatcgcag cggaggcggc tgttccgccc gcggcgcggg
    22921 tgcctgatca cctcagtggc ggaccccaat tcgcactggc gtcgatcgat ccgtcgctcg
    22981 acaggacgtg gcagatgtcg tccggccggc agtcggccgg gttcaaggtg gcgctgcgat
    23041 cgacgaggcg gatgagttcc tgttcgagcg cagcgagctg gacccgccgt tcgcgaactt
    23101 cggcgatctt gtcggccaga agcgctgtga cgtggccgca cggcgtggtg ccatcgtcgc
    23161 ggagttcgat cacactgccg atctcggcga gggtgagccc ggccgcttgc gcggtccgga
    23221 tgaagcgcag ttggttgaga gcagcatcat cgtaggtgcg gtagccgttg gctgaacgcg
    23281 gcggggcggc cagcagaccg cgtttctcgt agtagcggac ggtttcggta gggatcgccg
    23341 cagctgccgc cagatctccg atgcgcatac gacgacagta ctccttgacc ttggagtacg
    23401 gatcagggtt tatgttgcca tcaatcgaca cggtcgtcgc gaacatcgag aggaccacgt
    23461 gaccaagaca gaagccctgc cgacacaccg cggaggtacg agccgcaggc acgatccgct
    23521 gtttcccgtt gctgcaggtg ctgccgcgtt cggtgtgtgc tgcggtctgc cgctgctcgg
    23581 ctccttggga gctgcgggtg tgatcgccgg gctcgaagtc gggagttgga tcaccgttgc
    23641 gcccgcctcg ttcatcgcag tgatcggggt gcttcggtgg caccgccagc gggtgtgcca
    23701 cccaggtccc gggatagcga gcgcaggtct ggtatccaca gtcgccgttg gcgccacagc
    23761 gccccatcag caggctgggc agtgagctca tgctgcagca tcccaaccgg cccttcgagg
    23821 ccgtgtccgt cgtgtggcga ggtcggcccg atcgtcggct cggcaccggt tcgcccgcac
    23881 catcacgacc cagccgatgg cgcctggcaa cactgcccca cagcggggtg tcgggtcgtg
    23941 taccacctcg accagatcac ggtcgacgcc gacgcagtca tcgcccaggt cgctcacaaa
    24001 gcgactgatc gaccgacgcc ggtgtgtttc tgtttcgcgc acaccgccga cgacctcgtc
    24061 gccgacgccg cccggaacgg cggtgccagc accatcaagt cggcgatcaa gcacgccgtc
    24121 gccgaccggc gctgcgcctg cgagcacctc aatccgtcca ccaagtgctg cctggccgac
    24181 atccaccgct cgctcacagg caccgggccg gccacgacga cagatcgagt gagcccgaca
    24241 tgagcaggac gttcgacctt tacgacctga tcgtgatcgg cgccggcatg gccggcagcg
    24301 ccgccgcgga gaaatgcgcg accgccgggt ggcgcgtcgc gatcgtcgac gacctcccct
    24361 acggcggcac ctgcgcgcta cggggatgtg acccgaagaa gatcctgcgc cgaggcgccg
    24421 agatcatcga cgccgcacac ctcctcaccg gcaaaggcat cgacccggcc ggactggcca
    24481 tcgattggac ggcgctcagc gcccacatgc acggcttcac cgaccccgtc cccgccaaca
    24541 tggaaaccag cctcgggcgc cacggcgtcg acaccctcca cggcaccgca cgattcaccg
    24601 gcccggaccg gctcgacatc gacggcacca accaccagtt ccgacacgcc ctgatcgcca
    24661 ccggcgcccg accccgacca ctcgacttcc cgggcgccga tcacctcatc gactccaccg
    24721 gcttcttgta cctcgacgcg ctccccccac ggatcctctt cgtcggcggt gggttcatct
    24781 ccttcgagtt cgcccacatc gccgcgcgca cgggcaccca caccgtcatc gtcgaccacg
    24841 gcccccgccc gctgcgcggc ttcgaccccg acctcgtcga actcctcgtc acccgcagcc
    24901 acaccatcga catcgacgtg cgccgcaaca ccgagatcgt cgccgtgcac cgcagcggcg
    24961 acggctacca cgtcaccctc gacgatcatc gcagacagac cacgatcacc accgatctcg
    25021 tcgtgcacgg cgccggccgc caacccgaac tcgatcgcct cgacctcgac gccgccgaca
    25081 tcgcccacgg cacgcacggc gtccacgtcg ccagccacct ccagagcacc accaacccac
    25141 gcatctacgc cgccggcgac gccgccgaca cccccggtgc gccgctcacc cccgtcgcag
    25201 tgttcgaagg cagagtcgcc gcatccaaca tgctcaaagc cgccgccacc acacccaact
    25261 acagcgggat cgcgaccgcc gtgttcacca tccctgaact cacccgagtc ggcctgctcg
    25321 agaccgaggc ccgagacgcc ggcatcgacc tcgacgtccg ctacaccgac accagcggct
    25381 ggtactccaa ctaccgcatc ggcgagaccg ccgccgccgc caagattctc atcgaccgcg
    25441 ccactgacac gatcgtcggc gcccacatgc tcggacccga gtacggcgag ctgatcaata
    25501 tctgcgcgct cgccatcaag ctcggcctca ccacccgaca tctcaagtcc atgaacgccg
    25561 cgtaccccac cgtcggcagc gacctcggct ccatgctctg acctaatccc tgcaccgcac
    25621 caccccgcac ttccccggag agaccccggt cgccacgacg gcacccggcc atcggacccg
    25681 atggtcactg cccaacgtcg gggcacccac ccgatcacag aacgacatgg cctgcgcatc
    25741 ccgtcgcctg ctaccgactc cgctaccgaa ccccgatgat gaggagcgac aggtggcgta
    25801 cgtgaccgag aagcgaggcg tgttctacgc ggtgatctac caaggccgga acccggtatc
    25861 gggcagggac cgccgacgct ggcaccgctg cgacgaccgc gctggcgccg agcaactcgc
    25921 gaacgacctc actgagcaac gcgcccgaca acgccacacc ggctcgtcac tgaccgtcgc
    25981 cgagtacatc ctcggccaat ggctccctgc caaggaggcg acactcgcac ccacgacaca
    26041 tgcccgctac gtcacctcgg tcgagcacta cctgctccca cacctcggcg acacaccgct
    26101 tcgtcggctc cggaccgagc acctcgaggc gctctaccga cgcctcctca tcaccggcag
    26161 caggcgaggt gggccactcg cagcgaagac cgttacgaac ctccaccaga tcatcagatc
    26221 ctcactcaac gacgcagtcg gacggggcct cctggcgtcg aacccggcgc agggttcaca
    26281 cgtcccggac ccccgcaagc gaccatcgac tcgacgccgc gcccgctcgt ggaccgccgc
    26341 cgaactgagc gacttcctga ccgagaccgc cgccaaccgc tactcgatgt tgttccgact
    26401 ggccgccgcg accgggatgc ggcgcggcga ggtcctcggc ctgcgctggg acgacgtcca
    26461 cttcgacacc cgacgcatcg aagtgaccca ggccctcacc tcgatcgggt accgcctcga
    26521 gttttcacga ctcaagaccc gcaccagccg gcgcaacatc accatcgacg ccgacacgat
    26581 ggacctgctc gcccgatggc gacaggagca gacaacgggg ctcgcagagg ctggcgccgc
    26641 caacgctcac gggctcgtgt tcgtccgccc cgacggccag cccctccacc cacacagcgt
    26701 ctcgcaggcg ttcgaccgtg cacagcgccc cctcgacgtg tcaccgatcc ggttccacga
    26761 tctgcgccac acccacgcca gccttctgtt gcgtgaccgg gtcccgataa aggtcgtctc
    26821 cgaacgcctc ggccattcca accccgcgtt cacgatgacg acctaccagc acgtgcttcc
    26881 cggaatgcag gacgacgccg ccgccacatt tggacaactc ctctccaacc attcacgaga
    26941 agtcggcgag gtcgcggccg ccgcatagcc ggtaggcgtc ggtagacacc cggtagcggg
    27001 gccctgacgc gacagggccc ccgatcactc ggaggccttg ccatcactgc catgtcgttg
    27061 gtggcggggg tgggatttga acccacgacc ttcgggttat gagttcgacg acccttgtac
    27121 cggtctgtgc cgccgggtgc cgttccccct tggggttgca gggttctcgg gctctggcgg
    27181 tgccgtctcg tcccggcccg taccgcccgc ttctaccgga tctggcagaa gatctggcag
    27241 acgctcggat gcaggatcag ttgccgaatt tggccccatc gttggttctt cgagggagat
    27301 gacgggctgg aactcgatcg agtggaacgg ctcgtaaccc gccggccacg gcgcggtcac
    27361 ccggcacagc acccgcatgt gcggcgcagt gtcgggcccg ttgatgtgtt cgacggtgac
    27421 ggtcgggtct gcaccgttgt actggcgcag ctcgtggaga tgctcgacca ggtagtcggg
    27481 cacccatccc acgggcactc ggtcgtcgcc ggcgagcagc agtgcgcgtc cgttcatgac
    27541 gttgtcaggg tcgtccacga tcgcgagcag ctggccgtcg tggagacggt ccacggcctc
    27601 gggtgcgccg tcgaggtgac ggatacctcg cacgaagaac agcgacatcg cgtggtcgtc
    27661 cggggtgcgc tcgggcgctg cgaacacctc gatgcggtcg gtcgtgcggg tgccgttgct
    27721 gcgtccgagc acctcgaagg gatccgcgtc gacgtcgagg tcgaggcggc cgaggtagtc
    27781 ggggtagtcg gcgcggtcgc ggggcatcag ccggttcgcg aacataggga acagcgccgg
    27841 cgcgtcgtag cggcggtgca ggtcgggcag cccggggagc ggatggaacc catcgagggt
    27901 ctcggccagt ttcaggtagg cgaacgagta cgacgccgcg tcaccgtctc ggcgctgcac
    27961 gagcaggccg accgggatga tctggcgcgt ctcggggttg cgccacgcga cgaagaggcg
    28021 tcgctgcgga gactcggcgg cggtcatgga tccatcgtac gggtgaggtg ggacaggatg
    28081 cgcgcacgat tggcctcggc gacccgcgac gcgaactccc gggccggttc gctgatccga
    28141 aactcgggca cccgcgccac gatcctgcgc aggtcgagct gctccagcgg ttccaggtac
    28201 gcgcccgaga catcgggtcc gatcatgtcg atcgctgcgg ccgcgatctc caccgggtga
    28261 gcacggccct cgaagcgggt gcgtgccctg ccagcccacg cctccggggt ctggttccga
    28321 tcggaagtgt ggagcatttc cgagcgggca gcgtcgtcga gcaggaaccc gagactcgac
    28381 gcatgatcga aactcggcgc cagacgccgc tgcgccgcag ggttctcgat gacggcccag
    28441 ttctcctggt gacggtcggt gttgccagcg accgcgtcaa cgatgaggta gccggcgagg
    28501 cactcgaacg ccgacgaccc cggcaccgaa cccgcaacac cctcaagcga ccggcgtacc
    28561 gcgtcgaggg agtacccgct gcggtcatga gggtcggagc cgatgacgtc gatctcggcg
    28621 agtagctcgt tgccatgcac caggcgttcc gactccgggt ccagcaccga caagctgacc
    28681 gtgccgaaaa caccggcccg ttccgcaagc tcggtggtgg ccacgggtac gtccaggctc
    28741 cgggcgatct cggtcacgac tcgttccgcc cagtcgtcac ccttgcggta ctcaccgctg
    28801 ggcgacggat tccaggtcgc cgccttccac agccagagcc gatccgccgg gtcgcgcagc
    28861 cactgcttcg gcttcgtgcc caactgctcc tcaccaccag ctcgccacgc cgtcacatcg
    28921 agcagcatga actgtccgga ggaaggctca acaggcgacg gcaccacccc agtctcgccg
    28981 acaacagctg ggagtccgca cgacggcttc ggatacgctc gtctccatgt ggcagctcta
    29041 cctgtgatct gacggaccat cctgcggccc ttcggggcac gcagttgacg gcgatctgct
    29101 gacgcacgtc gccggtcaag tccgtagatc gcgcccggtc tcccccgaac ggagagggcg
    29161 caggaagaga agacccatgt ccagcaacga caacagtcgg gcgcgctcgc gttcgcaact
    29221 ggtggcctcc ggcgtgcgcg tcgaccgagg cgtccgccgg atcctcaccg acctctccct
    29281 caccgtcgct cccggcacac gactcgggat cgtgggcgag aacggccgcg gcaagtcaac
    29341 gctgctgcac gtcctggccg gccggctgac ccccgacgcc ggcacggtcc gccgcgtcgg
    29401 cagcatcggg atcgcggagc aggagatcac cgtccgcgag gaccgcaccg tcgacgacct
    29461 cgtcgacatc gaactcgccg acacccgggc tgcgcttcga cgtctcgatg acgccacccg
    29521 ctcgctgtcg gagaaccgag caggcgcaga tgccgcctac gcagcggcgc tcgaccacgc
    29581 cacggcgctc ggcgcgtggg acgcagaccg acgcgttgac gttgcactgg gtgaactggg
    29641 ggaaatcacc gatcgcggcc gacggttgac cacgatgtcg gtcggtgaac gctaccgggt
    29701 ccgcctcgcg tgtctgctcg gcgcgtcgca cgacttcctg ctcctcgacg agccgaccaa
    29761 ccacctcgac caacacgggc tcgacttcct caccgacgcc ctgcgagcca ccgacgccgg
    29821 ggtcgtgctc gtcagccacg accgggtcct gctcaagaag gtgacgaccc acatcctcga
    29881 cctcgacccc agcatcgacg aacgaccacg tctgtacggc aacgggtacg acggctacat
    29941 cgaaggccgc cgaggcgagc tcgctcgatg ggcacaggac tacgagcagc accagagcga
    30001 gcacgcacgc ctcgcaggtg acctgtccgc tgcacagaac cggctgcaat ccggatggcg
    30061 gccacccaag ggcaccggca aacaccagcg cgccaccagg gcaccggccc tggtccggtc
    30121 ggtcaaccgc cgtctcgagg acctccgggc gcacgaggtg accagaccgc agccgccgct
    30181 gcagttccgg atgcccgacc tccccgagct cgcgggtgtg accctgctcc gagccgacaa
    30241 cgtcaccgtc accgaccggc tgaaaacccc ggtgtcggtc gagctgtcct cacgtgaccg
    30301 gctcctgatc accggaccca acggcgcggg caagtccaca ctgctcgccg tgctcgccgg
    30361 caagctcgcc cccagccacg gcagcacgtc cgtcgccagc agggcccgcc tcggattcgt
    30421 cggccaggaa tccccatcgg tcgatcgacg gacggcgcgc gacatctacc gagcacgact
    30481 cgaccagctc ctcgccaacg gtctcaccga ggccgacatc gtgtcgctac gcagcctcgg
    30541 gctgttcacg acaaccgacc tgaaccgacc cgtcgacgag ctctcgatgg gacaacaacg
    30601 tcgcctcgac ctcgcccttg cgctcgccga tcgcccccac gtcctgctcc tcgacgaacc
    30661 caccaaccac ctctccatcg cactcgtcga cgacctcacc gaagccctcg ccaccacccc
    30721 ggccgcagtg gtgatcgtca cccacgaccg ccaactccgc atcgacctca gccactggcc
    30781 cacgatcgac ctcggccagg aacgggtcac ccggtagccg gcacccgagc tccgtggacc
    30841 acccggagtt cgttggaggc tcgtcgggtt ggtaccgaag tcgtcaggct gggccgatgg
    30901 cttacatcat caagcgcaag gaccgcttct acgtcgtcgc ctacgacggc ctcgacccgc
    30961 tgaccggcaa ggagcgacgc cgctggcacc cagccggccg ggaccgccac gaggccgagg
    31021 agatcgccgg tcggatccag aacgatgccg ccggagccgc tccgcctcga ggtggcccgg
    31081 tgaacctcgg cgagttcctc gtcgacacct ggcttccgat caagcgtcga cacgtccgag
    31141 cgaccaccgc gtaccggtac tcgtggatgg tcgacaacta catcagccca tcgatcggtc
    31201 acatccccct ccgccgacta cacgcagatc acctcgacgg cctgtacgac cagctcgcca
    31261 ccaacggggg ccgcgccggc accggactcg cagcgaagac cgtgcacgag gtccacatga
    31321 tcatccgttc atcgctcgac cttgcgatcc gccggcagct catcggcgtc aacgtcgcgc
    31381 aggcaacgaa cgcccgccac aagcgactag cccggaccgt cccccgctcc tggaccgccg
    31441 aggagctcgc taccttcctc gcggcagctc aaccacagcg gctctacccg gcgctgcacc
    31501 tcaccgcgct caccggcatg cgacgaggcg aggtggcagg gctcaagtgg tccgacctcg
    31561 accgggccaa ccagcgcatc tcgatcagcc gcaccatcca gagcatcgcc ggccaaccgg
    31621 ccgagttccc tgtcaagacc cgcaccagcc gccgctgcgt cgacctcgac gagcagacaa
    31681 cggacgtcct ggcccggtgg cgtcgacgcc tcacccgcga cgggctgccg cacgaacgcg
    31741 acgactggat gttcgtcaac cccgccggtc gatacgtgaa cccggaatcg atcagccaac
    31801 tgttcgaccg actccagcgc cagctgccgg acctgtccag gatccgattc catgacctgc
    31861 gccacaccca tgcatcgttg ctgatcatgg acggcgttcc ggtcaaggtc gtctccgaac
    31921 gcctcggaca cgccaacgtc gcgttcacca tgcacaccta tcagcacctt ctccccggca
    31981 tgggcgcaac cgccgctcga cagttcgctg cgctgctcac cgctcacacc cggtagacgt
    32041 ctaccggccg agatccccga caatccggga gaagcaggcc cgtccggtag acgacccggt
    32101 agacgcccac tctcgcccag aacgtcgaag gcccggaacc ccttgagatt ccgggccctc
    32161 tcgatggtgg cgggggcagg atttgaacct gcgaccttcg ggttatgaga ccgattctca
    32221 gcctctcctg gcgtcccgtt ccgtaccttc cagtacctct gaggtggggt ttcgcgcatt
    32281 cggcgtgcca gtccgtacca ctgcggacca ggcgatccca gcccgcggtg tagaagagag
    32341 tgtagaagcc ctgcccgctc ggcgcagcct ccgatcacgc gactcggaca gcgtccttca
    32401 gcttggcggg ggccgacgga ggccgacggc cagggtgacg agcccggctc cgtggatcgg
    32461 cggcaacgaa agcccgtcct tcggcggtcg gagccagccc tggcgagcgg gtcagctgcc
    32521 gagttcgccg cgcaggtggc ggttgccgct gcgcatgacg tcgtcgacga gcctgacgac
    32581 cgccggcggg gtccgtagcg ggaggtcgac gtgtgtccag acctcggttc cgaagccaac
    32641 cgcggccccg tcgggatcgg cctcggtccg ttggtaggcg acggaccgca gcgtcacgta
    32701 gctgcggacc gggccgggag cgatgactcc ggtcagggtg aggcagcgac cgtggtcgat
    32761 gagctcggtg gtgatccacc cgacggtgag ggcgtccgca gctcgagcga tcgtgagcgt
    32821 cccgtggtcg gcgtcgaagg tggcgccgaa ccaggccgcc agcttgacgg gggtgtcgac
    32881 gaaagctcgt acttcgtcga tgggggtgtt ggggatgatg acggcgtcgt cgatgatggg
    32941 cccggcgcct tcgagcttgt ggtgcaccca aggccacacg aagcgccgga gggtcacggt
    33001 tccccgctgt tgtcctgtgg ttcgggcgtg tggagacggg gctcggcttc ggcggcagtc
    33061 ttgggtccgc ggatggcggt gaagagcgcg gcggcggcgc agaccgcggc ggcgaggagc
    33121 agggcgggca tcatggcatc gacgaaggat tggcgggcgg cgtcgatgag ggttgcgccg
    33181 ttggggccca tctgaccggc ggcggcgagg gcgccgccga tgccttcggt gacggggtgg
    33241 gcgacctcgg gcgggagtcc ggcggtggct ggttcgacgt tggattggta ggcggcattg
    33301 aggaccgatc cgatgagggc gatgccgacg gcgccgccca tctcgcggac ggtgtcgttg
    33361 agcgatgagg cgacgccttg cttctcctcg ggtagtgacg aggtgatcgc tgcggtggac
    33421 gggctcatcg cgaggccgac gccggcgccg aggatgaggg tgccggggag cacggtgagg
    33481 tagccgccct gggggtcggc gagcacggcg aacacgacca ggcccgtggc catcgcgagc
    33541 atgcccgtca cgagggttcg gcggaacccg aaccgctcgg cgatggtcgg ggcgatggtg
    33601 gagaggggca tcatcatgac cgccatcggc agcagtcccg atgctgcttt gagggcggag
    33661 tagccgagga tggcttggag gtactgcacg agcaccagga acagggagaa catcaccgcg
    33721 aagaccacga agaggttcac cgagcccgcg gcgagccccc gcagggcgaa gatccggacg
    33781 tcgagcagcg ggtggtcgcg gcggagctcg acggcgacga aggcgacggt cgcagcgatc
    33841 ccggcgacga tcgccgcgag ggtgatcgga gccgtccaac cctgctcggg tccttcatgg
    33901 aaggcgagca cgagcccgcc gacggcgacg accgagagca gcgatccaac gacgtcgaag
    33961 ccggcttcgg tgtgttcgac ggagtgtggg acgaaggcgg cggtgagggc gaaggagacg
    34021 gcggcgagcg cgatcgggag cgcgaagacc caaggccagg tggcgttgtc gatgatggcg
    34081 gcagagacga acagcccgat gatgccgcct gcgccggcga agcctgccca ggtgccgatg
    34141 gctttggctt gttcctctcg cgggaagctg gtggtgatga ccgagagggt cacgggcatg
    34201 accatggcgg cagccacacc ggccaggacc cgcaagccga tgagcgtgcc cggcgtcgtc
    34261 gcgaacgcgg aggcgaggtt ggtggccgcg aaggcgacca gtccgatcat caggatgggc
    34321 ttgcgacccc accggtcacc gatggccccg accggcagga gcagcgcggc cagcgcgagc
    34381 gtgtagccgt tgatgatcca cagcaactgg ctctgggtgg cccccaggtc ctgggcgagg
    34441 gcctgctgtg cgacgttgag gcccgagacc gacgcgacga cggcgacgag cgcgacgcac
    34501 atggcagcga ggatgagccg gcgccgaccg gcgttctcga ccacgacgtc gtgttcgacc
    34561 ggatcgtgct ggccctccgg caggtcacct accggtccgg acggatccga cgtctgggtg
    34621 gtcatgaggt cccctctcgc tgatgtcgac ccgtggactc taggacatgc tgtgttctag
    34681 acgacattcc gtcctctaga gcgctgagag tagggttcga gagtggcccc caaccctgag
    34741 atcgaccccc gcgtcgcccg gacccgtcgc gatgtgctcg cggcggcccg tgaggtgctg
    34801 ctggaggacg ggtgggagcg ggtcaccctg gctcgggtag ctgagcgcag cggctatgcg
    34861 cgcacgacgc tgtaccggca ctggccccaa cgactcgacc tgctgcgtga cctgatcggc
    34921 gaggaggtgc ggctgctgca caccaccccg gcgggcgacc tgcgtgagga cctgatcgcc
    34981 gagatggaag cgttccaggc tgcgctgacg acctcgggac tcgggcgcat gatgatcgcg
    35041 atcggccagc aggctcgcga cgacgcggag ctggctgacc tgagctcgtc ggttcgggcg
    35101 gacggctcgg aggtgttgcg cgacatcatc gccgcgggcg tcgagcgccg ggagctgcca
    35161 tcggacctcg aacctgatgt cgccgttgct cagctggtgg gtccggttct gttccggttc
    35221 ctgttcgaga gcgaggacga cttcagcctc gcctcggtca cgtcggtggt cgactggttc
    35281 ctggccggca ccaacgccgc tcgaggtggc ggtgctcggg ttaggtccca cggggctcga
    35341 cgatgagcac ggtgccctgc gcgagggcgc agggccggcc gccgttgtcg acctcgacct
    35401 ggacgaccgc ggtgcggcgc gtgagcgaca cgatggtgga ccgggcgctc acggtgccag
    35461 cgcggaccgc ggtgaggagg ttgagcttga actcggtggt ggcggcccag tggccgcgct
    35521 cgatgacggg gtagagcacc gcgccgagga cgtgatcgac aagggcggat acgacacctc
    35581 cgtggagggt gccgaagggg ttgaggagtt cgggccgcac gtcgagctcg gcgagcatcg
    35641 tggcgggccc cacttcgacg gttcggatgc cgaggtagga cggcaggcca tcgatgctgc
    35701 cagcggtcat ggaggcggcg gtgtcggcgt cgtaccggtc gggggtgatg gctgcgggca
    35761 tggtgggctc ccggttcagg tggtggagtc gtcgttggcg aacttggtca ggtcgacggt
    35821 gaggaacagc gcctcggccc ggacgaagat cccgtcgtcg gactcgccgt gggcctggag
    35881 ccagagcttt cggtcggcgc ggtcgcgcaa cgacgcggtg acgcgcagct cctggtgaag
    35941 gggtgcgggg gcgacgtagt cgaggttgag gttcgcggtg taggccagct cgccgaggat
    36001 gggcaggacg tacccgatgg tctcgtcgac gatcgcggcg acgacgccgc cgtgggcccg
    36061 accgggagcc ccttcgaacg ccgggccgag cgtcgtggtc gcgaccaccg agtcgcctcg
    36121 ccggcgcacc tcgagaccga tgcccatcgg gttggtccgc cctgagacga ccgagtcgcg
    36181 gaacaggtcc atcggctcgc cgtcgaggcc gatcggttcg agggcgtcgc caccgagcca
    36241 gcgggcgaag cgggggctgg tggcgatctc tgcggtccgg tcgcgcgcgg gccgggccga
    36301 gacctcctcg gtcgcggcgg cggccaggtc ggcgatgcga cccgcgaggt cggcgtcgac
    36361 gcgatggccg acgaggctgt ggccgagtcg ctggagcgcg ctggccatgc gggcgcgggc
    36421 ttcgagctct tcgggggatt cgtcgaggtt cacggtgcag gttccttggg gcgggtaggg
    36481 ctcgggcggt
//
LOCUS       DAOQOD010000009        35730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_381_length_35730_cov_11.058893, whole genome shotgun sequence.
ACCESSION   DAOQOD010000009 DAOQOD010000000
VERSION     DAOQOD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 35730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35730
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_35730_cov_11.058893"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLZ72_02130"
     CDS             complement(<1..227)
                     /locus_tag="PLZ72_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNA35124.1"
                     /translation="MAEITASEIIDGVFVVTPDVYGDDRGYFVETYRRQWFPGGREMV
                     QGNRGNRIAGCIVGLHYHLHQADYWYVPFGHA"
     gene            complement(260..1366)
                     /locus_tag="PLZ72_02135"
     CDS             complement(260..1366)
                     /locus_tag="PLZ72_02135"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNA35125.1"
                     /translation="MKGLILSGGAGTRLRPITHTSAKQLVPVANKPILFYGIEDMVAA
                     GITEIGIITGETGPEVREAVGDGSRFGAEITYIPQDEPLGLAHCVLIARDFLGDDDFV
                     MYLGDNMLQQGLDGFVRVFEEDRARALEPTLHDDHVAPSAQILLCEVPDPHRFGIAEV
                     DSEGHVVRLVEKPEDPPSNLALVGVYLFTPAIHEAVASIEPSDRGELEITDAIQWLID
                     HGQRVRHDSLTGWWLDTGKKDPLLESNRRVLETLEPAIDGDVDDASDVDGRVVIEAGA
                     VVRNSRIRGPVIIGSGTEIVDSYVGPFTAIASDCRIESSDIEQSVVLDRSRIVGVARL
                     SDSLIGRDAEIHRSGLRPASTRLMIGDHCSIDLE"
     gene            complement(1457..2866)
                     /locus_tag="PLZ72_02140"
     CDS             complement(1457..2866)
                     /locus_tag="PLZ72_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35126.1"
                     /translation="MISGPPASDRSAAEAGWQDSWRGDALVVALVMVVVVAFNWLHVG
                     AYQTLSPVDEFQHVDYLYKAGKGQMVGRGDLVGDEAMHEEACRGVDAPGFEMPCKPIG
                     SYHASQFQEGGQNTAYIHSPVYYFGTAWVGKAVVKATPIDNLFKAARLLGPMWVLPAL
                     LLMVPTMRRLGAGIVARIAAGILAVTFPTVLHATSTVNPDATALLGGALVLCVPVLLA
                     GRWTRPGGRVVDGLAAAASMALKPSNLVVLIAGAWMRVDLSIGPARADSDVPDAQDRL
                     RPGPAVSRSVSAVLPMLLGAGVVSAVMLVVQARLASSDPDDIQMLHRYTVEAFPWGPF
                     AESFLSTGQFSAAGESSNYLAPFLRLPFIVVSSAVIAFASLVVTVAAALRSDRTAAMA
                     RSVLIATLLGGPALIAVNFILQGIFVYIPVRYYMSLIPGVVVVGAATVGRWRHGPHAL
                     AAVAVVWSGALAFALIAAQ"
     gene            2980..3510
                     /locus_tag="PLZ72_02145"
     CDS             2980..3510
                     /locus_tag="PLZ72_02145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA35127.1"
                     /translation="MTDPYVHPTAVVENGAILGAGARVWHHAHVRAGARIGAGTNLGK
                     NVFVDSGAVIGDRVKVQNNVSVYTGVTVGDDVFVGPSAVFTNDLVPRAFAEDWVVTPT
                     IVGHGASIGANATIVCGNDIGEFAMVGAGSVVTRSVRPHQLVVGNPARHAGWVDRRGH
                     VVSRDDEPPPAELLGR"
     gene            complement(3566..5617)
                     /locus_tag="PLZ72_02150"
     CDS             complement(3566..5617)
                     /locus_tag="PLZ72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HNA35128.1"
                     /translation="MSCRPHGRILRRSVVVFGFGLLLSVVPLPVLHFETESVGAVEDV
                     PTVTAMTAAQIDAARAATGEPVGADRVVAADDSAGEFSAIGFTFDSEPTAPVLVRVRD
                     ADGNLGRWQALETDDGEGPDAGSAEAGRSGTAPLWVGSGTGYEVSLGAGDAVGAKVVT
                     VRDELRRVTADSTPVAGAATPPPFGIRTRDVWGARAPLSTSYASTVKLAVVHHSDSGN
                     SYGPGDVPGILRSIQAFHMDGRGWSDIAYNFVVDKFGTVWEGRGGGIDRPVIGAHAMG
                     FNTGSVGVMVIGDYTQATPTAAALESVSSVIGWKMAQHLADPAGTVAFTSGGSTKYPA
                     GQLLYLPGVVGHGDVGQTSCPGSIANSLGSIRRRAQEWATWIRTTSVPVGSLDSLDGG
                     GGTVTARGWAKDLSTATAPQVELTVGASSRTVTANRSRPDVAAVHPSYGSSTGFEATL
                     AGVPPGKQMACVTVHDADGGPPTSLGCRRVLVDDPTGLSPTGSITSIVGLIGGFRVQA
                     STADSDGAAPRSIVVYVDGSPVLTTTTDSHGRLDVTRAGVLGGRREVCVIVGNRGGGQ
                     DVVADCAFVDVLGWSPMGNLESVTRSGRHVALSGWAFDPESHAPISVAVLFDGRRVAV
                     VPADRSRPDVAASKGMGSNSGWGAELWALPDGAHTVCAVALNVGGGADQNLGCRNVVV
                     K"
     assembly_gap    5665..5764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5886..7667)
                     /locus_tag="PLZ72_02155"
     CDS             complement(5886..7667)
                     /locus_tag="PLZ72_02155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA35129.1"
                     /translation="MAEDLDPYVRVVVLNWNSAWYTRRCLDALAATEYPADRVEVVLV
                     DNGSVDGSLERLRDWFPDVRVVANGANLGFAEGCNRAMRDRDGVDAVALINNDAVPSP
                     GWLRPLVDALGAAADIGAASSALVLEPGFVPVDVEVDADGTADVVAVRVDGADVTGAL
                     RFDGFEGVSDAAWPLDITYRLNAGAGRIWIPAGSGDLDVEVELTGTGAVEVRIGDRVG
                     SSARPGTATLRSGLERTRLINGLGTARNERCEGYDRLYGRPVDLLDGMGADPVPVDGF
                     CGGAVLLRSRMLDSVGLFDPRLFAYYEDTDLSWRATRAGWRIVAVPASRVEHAFGASG
                     GSRARGFFHLDRRNWWLTAERNGTPAQRTVVRREVRREIRTALRANIAGRLKRRHLPS
                     LQLLSAWARIVADHTVEVRRRNHPVVGPTGMRPTDRVVGRFQPRPAPSLPRPRPWGPL
                     AVALDVTPLVAEWPTAGWDEGTVTAGRILARSLLQDHAELDPLPVVADDGARVATPAE
                     VAIVLGVAAPLIPPRDGPISVTDHDRIGLRLVPDATAGRLAVEVTPVGGGPSRRVLVD
                     RPAGDPGEAEPVIAALREAVSTEAHRR"
     gene            7748..9211
                     /locus_tag="PLZ72_02160"
     CDS             7748..9211
                     /locus_tag="PLZ72_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35130.1"
                     /translation="MQGGDAAAALDAHPDRPPEPDTVIGADLPGSAGLVSTPLPRGGA
                     AWLRSALTGGQSSLTLGLLFDGVASYVFLSAAARDLGPYRFAMVSVLWVVLFLIGNGL
                     FIPVEQELSRSIAARAVRGSGWASLVRRVTVASGAVLAVAIGTGILARGHLADSLFRG
                     DQTFVWLLLGGLVGVWLMFVLRGVLSGERRFHAYGLMFVADATAKALPGVGLLWWGAL
                     RPSSYGLVVAGSAYAGIVVAYACLRWRRAQGSASTSDVLTPDPEAPDVAAPWGRLSSS
                     MGFLLLTSFLSALAINIGTVAIEVTGTRVDADKAGIFLSGLVIARIPLFLFQAIQAIV
                     LPRLSLLAARGDLGGFRSDLRRLYAAMGTTTVVATLGAAALGPLAVRVLFGEEFALLG
                     ARDMALLTLSSLLMTVALTLNQAQIALHHQRQTGWPWGVATAAFLAIVTTNGRDVFLR
                     VELGMVAAGAVAALIVTILVQRELRHPDHLRSDTPAL"
     gene            9229..10425
                     /gene="lhgO"
                     /locus_tag="PLZ72_02165"
     CDS             9229..10425
                     /gene="lhgO"
                     /locus_tag="PLZ72_02165"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNA35131.1"
                     /translation="MGSALDVVVVGGGIVGLATARALLLDRPGSAVVVLEKESAPARH
                     QSGRNSGVIHSGIYYPPGSLKALLCAAGRRSMEAYARDHGIPVEITGKLIVATRIEEL
                     DRLDALAERGRAHGLTVRTMPAAEAVEVEPHLRCVGALHVAETGVVDFPAVCAQLARD
                     VVELGGEVRTSCRVTAVATVPGGQRVTTTSGELRTRRLVNCGGLQSDLLAAAAGANVS
                     GRIIPFRGEYRELVPAARPLVRGLIYPVPDPRFPFLGVHLTRGVDGGVHAGPNAVLAL
                     AREGYRWIDVDIGETVDTLRSPAFRHLARRHLRDGIGEVVRSLSERRFLAELQRLVPE
                     LRAEDLQPAPAGVRAQAVLDDGTLVDDFLLVSGPGALHVLNAPSPAATASLEIGAEIA
                     GRLTTP"
     gene            complement(10513..11685)
                     /gene="moeB"
                     /locus_tag="PLZ72_02170"
     CDS             complement(10513..11685)
                     /gene="moeB"
                     /locus_tag="PLZ72_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNA35132.1"
                     /translation="MANFRDLLNAAKAEIREVSTAEAEEMIDGGATLLDVREADEFEQ
                     GAVPGSVFLPRGHLESQVEGRLGDKAGPVVVMCAGGVRSAFAARTLGELGYTDVVSMA
                     GGFNRWKDEGRNWRVPEVLNPEQRNRYSRHLLLPEVGEEGQKKLLGARVLLLGAGGLG
                     SPAALYLAAAGVGTLGIVDMDVVDDSNLQRQILHNVDRIGERKVDSAKKTLTALNPDV
                     DVITFDVRLDASNIADIVGQFDLVVDGADNFPVRYMLNDASVKLGVPVVHGSIFRFEG
                     NVTVFDPKNGPTYRDMLPEPPPAELAPSCSEAGVLGVLPGIVGTLQALEALKLILGIG
                     DPLIGRYLAFDALDMTFREFKIRKDPSNPITWENRDQIVVTELEGACQPAPLQPPS"
     gene            complement(11800..12579)
                     /locus_tag="PLZ72_02175"
     CDS             complement(11800..12579)
                     /locus_tag="PLZ72_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNA35133.1"
                     /translation="MPDGARELESARDLLAGSRRVVVLSGAGISTDSGIPDFRGPDGV
                     WTRDPEAERLATIDVYLAERSVRERAWRRRMDHPAWTAEPNAGHRALRGLDLQGRMHL
                     LVTQNIDGLHLAAGHDPHDVVQVHGSLPESHCVRCDWSGPTRDVFDRVRSGELDPACP
                     DCGGVIKPRVVFFGEALVEADIARALDAAGEADMLLAVGTTLAVYPVAHMVPIVVDRG
                     APVVIVNGSETEMDDMATVVLRGSLSELLPALVAGLPAILG"
     gene            complement(12589..13344)
                     /locus_tag="PLZ72_02180"
     CDS             complement(12589..13344)
                     /locus_tag="PLZ72_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA35134.1"
                     /translation="MTGSVVLTGRPDGTTARSVEVLAGLPAEFRLIGGLAVLCRVGVP
                     HRATVDLDALARGLDSFDPGLRSVALSAPGGGQYLMEGRLELDVIDVGDADAHDLVAE
                     LVAAGEPLTDLEVNAVAHAWAHDVATGLDLVVVSELDGTVLASAPDRLVASPGGLVAM
                     KATTIPLRTSSKPEKRASDLYDLGRLLGSTGGWAEELAGAPGVLADAVRERVRGWFVD
                     PGGRDRTFRDTRRFDEVRLDLDDVADAVQEAWP"
     gene            complement(13341..14321)
                     /locus_tag="PLZ72_02185"
     CDS             complement(13341..14321)
                     /locus_tag="PLZ72_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35135.1"
                     /translation="MFVTSRTPIGRELIEACRAAGVDVTTPAGRLTVDGVVLDIDVIE
                     RSHPHPGELAALVESRETVEPGLVVADRISAAGRDVLRAAGWSWLDRRGHLRVWRPGL
                     RLETDVALGGSDGRGTRSSPWTAVGLEVALHALCHPDDAVRARAVAAEIGRSVGGTQE
                     VIGRFAEHGLIGRTSRRPLLPDLFWETAAHWPDDDWTAVAAELPDVVAVLGADALVRV
                     DERAATLGGARIAAAASLPARCYVHTRGALRRVRSAFGSSGAPRTFLRMSPVGWLPEL
                     EGFGPDDDHPWHVAAPMVCALRLARDPARGREIVEDWGVVPGAAASGDAP"
     gene            14381..14635
                     /locus_tag="PLZ72_02190"
     CDS             14381..14635
                     /locus_tag="PLZ72_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNA35136.1"
                     /translation="MSTLVVNTHEAKSRLSALIRAAEEGDEVILARNGRPVARLVPWT
                     TSAPTRSPGAWSGRFEVPDDVVGSDPDVIEMFDRSADSTL"
     gene            14632..15015
                     /locus_tag="PLZ72_02195"
     CDS             14632..15015
                     /locus_tag="PLZ72_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35137.1"
                     /translation="MRRLLIDSHVALWWLSGAAALGPTSRAALTDADVVLYSAVTPWE
                     LGIKESLGKLTLPEDLVDALTNTGFTPLDITADHARRAAALPPHHRDPFDRMLIAQAQ
                     LESLVIVTADGSFGDYDVDRLDART"
     gene            15258..15743
                     /locus_tag="PLZ72_02200"
     CDS             15258..15743
                     /locus_tag="PLZ72_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35138.1"
                     /translation="MTRTAVDLPPHRGSGPIDPSPGEPPERLTVKPWWDSRLALVGVR
                     PDDPYVERFWLAIVGPSTVVLLRRFTRGLDEHPEGFRVGLLETSLALGLGRGTGRNAP
                     ISRTLARAETFGLMRRTGEHGLQVRTHLPVLSARQVRQLPPVLRRVHADWVRIHRTDR
                     P"
     gene            15827..16081
                     /locus_tag="PLZ72_02205"
     CDS             15827..16081
                     /locus_tag="PLZ72_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35139.1"
                     /translation="MTGEISQRELRNDSGRIMRALTEGESFIITRNGEPIGELTPRRR
                     RRFVRATAVVELFRSAPPVDLDTLRRDLDALAPQDAVPRG"
     gene            16074..16484
                     /locus_tag="PLZ72_02210"
     CDS             16074..16484
                     /locus_tag="PLZ72_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35140.1"
                     /translation="MGENRHERGVIDTSVVIDLESIDASALPREVAISSVTLAELAAG
                     PHATDDPAERARRQDRLQRVEAAFDPLPFDLEAARAYGRVFAAVAGAGRTPRGARAVD
                     LLIAATACAEGMPLYTRNPRDFRALDGLVDVIAV"
     gene            complement(16533..17621)
                     /locus_tag="PLZ72_02215"
     CDS             complement(16533..17621)
                     /locus_tag="PLZ72_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35141.1"
                     /translation="MALEGVSRTFAADPPAVALDGVDLAVAAGSLTAVLGPSGSGKST
                     LLGVLAGTVRPDSGTVHVGGREVDAPGRHVPPEHRRVGLVPQDGTLFPHLDVAGNVAF
                     GLSGRPRSQRRERVAEMLELVGLASLSSRRPDELSGGQQQRVALARALAPAPDVVLLD
                     EPFSALDTSLRSSLRAEVARVLSDAATTAILVTHDQEEALSMADRVVVLRDGRVVQDA
                     TPTEVYRSPADEWVAGFVGDAVLLDGELLGHGGGGARVRCVLGDLDVDVARNGPSPRG
                     RVRVFLRPEQVWRHLPGDRGPVGEAPVGRVRRVRFTGAEMVVTLEVAGVDVTARWPSS
                     LGNVSVGDRLALEVLGRGVAFPRDPDSA"
     gene            complement(17680..19212)
                     /locus_tag="PLZ72_02220"
     CDS             complement(17680..19212)
                     /locus_tag="PLZ72_02220"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNA35142.1"
                     /translation="MTEPVDVKVAKSTPSGATAVAVTAHSDRLNRVPGLRKAALERAG
                     FSGAVGTTATFDDGERATVVVGLGPSTTSGSARTDALRRGAAAFARAVGRHRRVAFEV
                     PDPSAVDDLAAVARALTEGLVLGSYRFDDLRSAANVKPGLATATLVVGDDNAAVRSAR
                     DGVRAGAAVADAVCFARDLVNTPGGTLTAPELADRAAVRATAAGIGVEVLDLAAIAEA
                     GLGGLIAVNRGSTIEPRLVTLTYEPDGADDDTPTVALVGKGITFDSGGLSLKPAASMM
                     DMKCDMGGAAAVISAMCALPALGVGVRVVGITPMTDNMINGGAQRPGDVYTARNGTTV
                     EVLNTDAEGRLVLGEALILASEREPDAIVDLATLTGACMVALGDRIAGLMSNDDDFRA
                     TVAEAAATAGERVWPLPLPEDYRPRLDSAVADIKNITSQTGGGTLTAGLFLRHFVGDG
                     IPWAHLDIAGPAFLQEPDGEQPKGATGFGVRTLLALLEGWDAGGAGDSPDDDGDEDTD
                     DA"
     gene            complement(19300..19884)
                     /locus_tag="PLZ72_02225"
     CDS             complement(19300..19884)
                     /locus_tag="PLZ72_02225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNA35143.1"
                     /translation="MDIYTRKGDDGTTGLLYGGRVRKDDPAPAAYGDVDEAQSAIGVA
                     RAHAAAAVPDGAHVADTADELVQLLVRIERDLWVAMSELATASGNRHKLSAGTSLVTE
                     SMVSDLEATIDDVMGRFEAPREFVVPGETVVAAHLDVARTVVRRAERSALAAAEDGSQ
                     VVPYLNRLSDLLWALARWQEGRSLTTRSVNDGSS"
     gene            19959..23561
                     /gene="metH"
                     /locus_tag="PLZ72_02230"
     CDS             19959..23561
                     /gene="metH"
                     /locus_tag="PLZ72_02230"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNA35144.1"
                     /translation="MADTAPSSPASPYLAAIADHVVVFDGAAGTWLQEQDLTVEDYGT
                     PELEGCPEILVETRPELIARMHAEYLEAGAEVIETNTFGGMRATLGEYGLGDRTVELN
                     RMAAALAREVADRHSTPERPRFVAGSIGPGTRFASLGQVRYAELRDQVAEQARGLLEG
                     GVDLLLIETQFDLLGIKAAMNGCRAAMEEVGRTVPIQTQVTIELTGRMLPGTEIGAAL
                     VALDAMHPDVIGINCATGPEEMGEHVRHLAQYSRIPISVLPNAGLPSVVDGHMHYDLT
                     AEDFVAHQRRFVEEFGVEVVGGCCGTTPEYIRQLAEMVASTPRAERTPTFEPGLSSIY
                     SPVILSPAEDDAAGSASTNLIMIGERTNANGSKKFREAMLAGDWDTCVQMAKDQVKEG
                     SAVLDVCVDYVGRDGTADMDEIAQRFATQSSVPLVLDSTEPPVLEAGLQWVGGRAVLN
                     SANLEDGEGPGSRFDRVMTLARTYGAAVICLLIDEEGQARDVEWKLRVARRIHDLAIE
                     RYGLGASDLVFDALTFPLSTGDDDLRGDAMATIEAIRRIKAELPGVHTTLGLSNVSFG
                     LSPAARHVLNSVFLHECVQAGLDSAIVHASKILPLNRIPDEQRDVCLDLIWDRRGTAG
                     ALSDGDDSYDPLARLLEVFSDVQVAEAVTEDRSGWTVEQRLSQRIIDGDRDGLVDDLD
                     EALAAGLSALAIINDVLLAGMKVVGELFATGEMQLPFVLQSAETMKASVSHLEQFMEK
                     ADGDAGKGRIVLGTVKGDVHDIGKNLVDIILTNNGYEVHNLGIKISIAEMIDKALEVD
                     AHAIGMSGLLVKSTLIMRDNLEELNERGLAEIPVLLGGAALTRTYVERDLREVYEGRL
                     FYGKDAFEGLRVMDRLGDIRRGGEDDPGWGREPGGRDIPARRVHRDDEPEVELPERSP
                     EVMTDNPVFVPPFTGSKVIKGIPVDDVAAFVNLTGLYRNQWQFRPDKRADGSVETDDE
                     FKERMRPMFREQLAAAKSEGLLVPQVVYGFFPANADGTDLVVWTDESRSEERARFPFP
                     RQRKEPFLCIADFFRPVDSGDLDYVAFQIVSMGSAVSERTAELFAANKYQDYLFLHGL
                     GVEMAEALAEYWHRRIRQEWGFADEDPPGADAAAPDNGALNGLFRQRYRSGRYSWGYP
                     ACPDLEDNQTVAELLGAERIGVTCDEDTGFQYHPEQTTSALICHHPRAKYFVAR"
     gene            complement(23566..24492)
                     /locus_tag="PLZ72_02235"
     CDS             complement(23566..24492)
                     /locus_tag="PLZ72_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35145.1"
                     /translation="MLRRRGLALFATLTLIAVALAGCMPGAGRTWNGPDPVRTIPFKG
                     LGAWWDVWDWSPTFTGGSAPQDLADVDRLAAAGVQTLYIQTATYRHPDDVLDPTLLRS
                     IVRRAHLRNMKVVGWYLPQFLDMEVDIRRMSAITGLGVDGIGIDIEATDNPDVADRTD
                     KLMAEVRFLRALHPDVPMAAIPVTPIIWEQLNRSWWPNFPYRELSRYMDAWMPMAYWS
                     YRRAGSFPEWGDPYLYTAESVTRLRTLTGRPNLPVHPIGGEGTGMTVDDAARMAIAVS
                     DTGAIGGSVYDDRITPQAVYPALGFMRRAQVK"
     gene            24644..25984
                     /locus_tag="PLZ72_02240"
     CDS             24644..25984
                     /locus_tag="PLZ72_02240"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HNA35146.1"
                     /translation="MDRTRLASLMAAEHERFVSRTPRSAELHRQARRSLLAGVPMNWM
                     TRWPGPHPVFVDDASGARFTDVDGNTYIDLCLGDTGAMTGHALPATVRAIEQQAPRGI
                     TTMLPSADAVAVGDELRSRFGLPVWQIAMTATDANRFALRIARHVTGRRKVLVFDWCY
                     HGTVDEALNVLDGDRVVPRPGNTGWATDPELSVEVVQFNDVPALERALSTGEIAAVLA
                     EPALTNIGIVAPDPGFHDALRRLTSDNGTVLIIDETHTICCGPGGATREWGLEPDMFV
                     IGKPIGGGVPCAAYGMTTDIAERVIDLVDGPDSDVAGIGGTLTANSLSLAAVRATLAH
                     ALRDEDFAVAVPLAEAWADGVRSVIDRHGLPWTVNRLGCRAEYWFCPAPRNGADAAAA
                     VDHDLDAFLHLWCLNRGILLTPFHNMALFCPAHTISDVEHHTAVFADAVAALTA"
     gene            complement(26007..26924)
                     /gene="ligD"
                     /locus_tag="PLZ72_02245"
     CDS             complement(26007..26924)
                     /gene="ligD"
                     /locus_tag="PLZ72_02245"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNA35147.1"
                     /translation="MADVTPVSLDGRTLTLTNLDKVLYPATSTTKGEVLDYYARIAPV
                     MVAHTRGRCMTMRRYPDGVEGPSFFEKRCPSHRPEWMPTAVGPGDRKGELRYCRFDEP
                     AAIVWAANLAALELHAPMARSDDLAHPLMCVFDLDPGPGTGIPECAALALEIRDLLAA
                     ADLDCVAKTSGSKGMQVYVPLNSPGPDAADHEHCASFALAVGQLLAQRHPRGVTTTMA
                     RAQRSGKVFVDWSQNALHKTTVCVYSMRARPEPTVSTPLHWAEVAAAADGSDPLVFRI
                     GDVLERVAEHGDLFATALEAVQRLPAPRS"
     gene            complement(26967..27875)
                     /locus_tag="PLZ72_02250"
     CDS             complement(26967..27875)
                     /locus_tag="PLZ72_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNA35148.1"
                     /translation="MPRAIWSGSISFGLINIPVKLFSAVTERTVRFNQIDTRNMSRVR
                     TRKVNAEDGSDVPADVIARGYEVSKGRYVIVTEDELASLQPRATHTIDLEEFVDLSSI
                     DPMYFDGAYWVAPDDRAAKPYALLVEAMEKAGKVAVARFVMRAKQYVAVMRPRDGRLV
                     LSMMVYEDEINPATDIDEFDQLDSVEVSERELTMAEQLIESLSAEFVPGSFRDSYRDE
                     LLDLIAAKDAGEERVLAVAEGPAEDEVIDLMAALEASVAAAREARGRHPSTADDNPVP
                     VEDAAPEESPAEEPAPSKPAATRRSA"
     gene            complement(27974..28684)
                     /locus_tag="PLZ72_02255"
     CDS             complement(27974..28684)
                     /locus_tag="PLZ72_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35149.1"
                     /translation="MTDPAVAPSVPSAAPPARSVGASMPGPAHIAWWSGGADPARQPI
                     VELDVGPDDGDASLLSARVDGLRAEDPDRTIALVHPGAGGSAATTDRWRSRLGEVVMC
                     LAGRADGFTGDTTPTSLARLRRWTADTLRRAAVPRSTAADVVLVVDELVSNVEEHAPG
                     WMTVDLELLDHGVLVVVSDPHPTRLPAPGQPPPERPAGRGMMIVAALSAQWGVILDGG
                     TKAVWAELEWSPRAWSSS"
     gene            complement(28742..29596)
                     /locus_tag="PLZ72_02260"
     CDS             complement(28742..29596)
                     /locus_tag="PLZ72_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35150.1"
                     /translation="MSLNVATFLLQWATGGLAFLWVTTRRREVSLGYGWLLRSTYGLM
                     ALGALVAGLRYDPLLWRDLASAAVVVVTAAVLVVSVLRRSAGVALQRERSQARSERVA
                     QMTGIDRAEQTFDPDAPEFPPDLDLIAPTIGLVGVVAGGLDVGGPPWLGVARTVAGAA
                     FLGSVTDAMLLGHWYLTQPGLPRDPLNELVRWVGWTWVPEVALQLVPVGMVAVLNGTI
                     DDGYGGMLGWFWLACAVTTIVLVVVTRKALQERYYSAVMAATGLLYLAILTAFGTDLV
                     ARALLAGT"
     gene            complement(29692..30855)
                     /locus_tag="PLZ72_02265"
     CDS             complement(29692..30855)
                     /locus_tag="PLZ72_02265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA35151.1"
                     /translation="MTDSGASEGTVTEQTVTEGIVTEPFEELVERLTEEADEGFFHTA
                     AQIVVVADGECVLDTAVGTTHLEQPFTRDTLSALYCTAKPLVAVAVLRLVAEGELSLD
                     DRLGDVIDGLDELGAPWIAERTVEQVLGHTAGLHVMNTVFARILPERSREGWLVASEP
                     PMAWRYGVDRAYAEFGGWYLLGRAVEALTSASYSSYVRDRVLVPYGVDPDDLVVRFDP
                     ATFDRLHDRISATYDLTMDQPVPLLAEVGPETACEWNPAFGTYGTMSAVATFYQGLLG
                     DLDGAEVVLPPDLLADAVTARLPLNHDVTLDRDAGFGLGFMTPLSSHFFGDGPSPRAF
                     GHAGQGGTSFAMADPDNGVVLSVLFNAGLDADTALSFRRVNLVDAVYRALAQL"
     gene            complement(30921..32189)
                     /locus_tag="PLZ72_02270"
     CDS             complement(30921..32189)
                     /locus_tag="PLZ72_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35152.1"
                     /translation="MYEWSEEQLMVRAAVRDFVDKEIRPHRDALEFGDLPPYDLLRKM
                     FATFGMTEMQKANFDSRIAAERAAADGTASQDAGTGGDAKERATRDANAIAFQIIPII
                     ELCRVCPGMVTAMGVSMGLTAASVMSKGTIAQKERWGRDLLTLEKIGAWAITEPGSGS
                     DAFGSMKATARRDGDEYVLNGSKTFITNGPYADTIVFICKLDEGNPPEERTVVQFVLD
                     AGMAGLEQSKPLRKMGMHSSPTGELFLDDVRVGSDRLLGESEEAYKGAGGRQASKATF
                     GQERTGVVAMALGIVERCLELSVAYANDRVQFGQPIGQFQLIQLKLAKMEVARMNLQN
                     QVFRNIEMAAAGAPGSFAEASAGKLYAAQAAMEVALEAVQIFGGNGYMAEFEVEQLAR
                     DAKVLQIYAGTDEIQVSAVARALLAEQAGA"
     gene            complement(32251..33015)
                     /locus_tag="PLZ72_02275"
     CDS             complement(32251..33015)
                     /locus_tag="PLZ72_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35153.1"
                     /translation="MGFVAQFRRSVDQESTREDGSGQLDLHLPEYATGTVHHQAILED
                     GTVVHHHQRFESGVLVDWIEDDEPGPWALIRPGSPLRPCPGGVADPDDVTGAALRLGD
                     EIRPLPLLDDLPAPAFESLARVPHATARLRFEVFATPVGTQRCDVRYHDGSRTAWLVS
                     DWTDPTEDEPAFGAPEMHVSMSFRNYLKMRSGQATALEAIEDGGSVDARWTLLLLLHG
                     MIQSPEYVAAYRGLPVLPDELGWWGEVAPHIPPAED"
     gene            complement(33062..34906)
                     /gene="lipA"
                     /locus_tag="PLZ72_02280"
     CDS             complement(33062..34906)
                     /gene="lipA"
                     /locus_tag="PLZ72_02280"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNA35154.1"
                     /translation="MTALRIRWLGTVPYREAWALQRALHDGRSGPEIGEHLLLLEHPP
                     TYTLGRNADGRHVLVDPGSVGAELVEVDRGGDVTFHGPGQLVAYPIVTVDPAAAGEPA
                     ARSTPALGVRDYVHLLESVLIDTIRQVDTAGRLGPDDVGRHDGYPGVWIRPGTADARK
                     VAQVGVRVERGRTMHGVALNVDPDMTFFEHIVPCGITGYGVTSLAREGIDASMAAVVD
                     AFAAAFAAGWAARGGQSDGLDVDRSDVVWRRSHAGAAPDDSDLAPFSRGAGPGRPTTG
                     ANAMVGGAGTGTGGSSGVSTAGGTSVRLLGRLAEAGVTDRVELTDRKPDWMRARVRHD
                     ADVLGVKRTVRDLGLVTVCEEAGCPNLSECWSDGTATFMVCGERCTRACGFCLVDTRH
                     PEPPSPDEPERVADAVARMDLSYAVITMVARDDLADGGAAHVAATVAAVRRRRPGTRV
                     EVLISDLKGDAAALEVVLAAEPDVVNHNVETVARLQRAVRASAGYARSLALLGRTALA
                     GHTTKSGLVVGLGETRDEITSTLADLAAVGVSIVTIGQYLRPTSHHLPVARWWTPDDF
                     AGFAAVGRSLGIAHVESSPFTRSSYHARAAADSADPVTGPAAEVVVRS"
     gene            complement(34908..35558)
                     /locus_tag="PLZ72_02285"
     CDS             complement(34908..35558)
                     /locus_tag="PLZ72_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNA35155.1"
                     /translation="MATSPHSITMVEVDYEAVERVRRVERSAFKDQEGFALTYLPFVS
                     RAVVDALHEFPHMNASVADGHLVVHNEVHLAIAVDLDYQGLLAPVIADAHDRRLRALA
                     REITDLAARARSRQLGPDELTGGTFTISNSGSYGTAMVIPIINQPQVAILSTDGVSRR
                     PVVVLDEHGNESIGIHSVGNLTLSWDHRAFDGAYAAAFMSRLKEIIETRDWAVELG"
     gene            35551..>35730
                     /locus_tag="PLZ72_02290"
     CDS             35551..>35730
                     /locus_tag="PLZ72_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35156.1"
                     /translation="MAIDMTMWSPVMRRILFNGSTVSPLPGRIGPLDGGAGRDRGAAG
                     ATAAGLGAGLGAATTG"
BASE COUNT         5149 a        12841 c        12730 g         4910 t
ORIGIN      
        1 gcgtggccga acggcacgta ccagtagtcg gcctggtgca ggtggtagtg cagaccgacg
       61 atgcagcccg cgatccggtt gccccggttg ccctgcacca tctcccgtcc gcccgggaac
      121 cactgtcgac ggtaggtctc gacgaagtag ccacggtcgt cgccgtagac gtcgggcgtc
      181 acgacgaaca cgccgtcgat gatctcggat gcggtgatct ctgccatggg ttccagtcgt
      241 gatcggtggg ttccggcgcc tactcgaggt cgatcgagca gtggtcgccg atcatgagac
      301 gggtcgacgc gggacggagg ccggaacggt ggatctcggc gtcacggccg atgagcgagt
      361 ccgacaggcg ggcgacgccg acgatacggg accggtccag aacgaccgac tgctcgatgt
      421 cggatgactc gatgcggcaa tccgacgcta tggcggtgaa tggaccgacg taggagtcga
      481 cgatctcggt gccggacccg atgatgaccg gaccgcggat acggctgttg cgcaccacag
      541 cgccggcctc gatcacgacc cggccgtcga cgtcggacgc gtcgtcgacg tcgccgtcga
      601 tcgccggctc gagcgtctcg agcacccggc ggttggactc gagcagcgga tccttcttgc
      661 cggtgtcgag ccaccagccg gtcagcgagt cgtggcggac acgttgtccg tggtcgatga
      721 gccactggat cgcgtcggtg atctcgagct caccccggtc cgacggctcg atcgacgcga
      781 ccgcctcgtg gatggcgggg gtgaacaggt acacgccgac cagcgccagg ttggacggcg
      841 ggtcctccgg cttctccacc aggcgcacga cgtgaccctc ggagtcgacc tccgcgatgc
      901 cgaagcggtg cggatccggc acctcgcaca gcagtatctg ggcggacggg gcgacgtggt
      961 cgtcgtgcag ggtcggctcc aacgcccggg cccggtcctc ctcgaacacc cgcacgaagc
     1021 cgtcgaggcc ctgttgcagc atgttgtcgc ccaggtacat gacgaagtcg tcgtcgccca
     1081 ggaagtcacg ggcgatcagc acgcagtggg ccagaccgag aggttcgtcc tgggggatgt
     1141 aggtgatctc ggcaccgaaa cgtgagccgt cgccgaccgc ctcgcgcacc tccgggccgg
     1201 tctcgccggt gatgatgccg atctcggtga tgcccgctgc gaccatgtcc tcgatcccgt
     1261 agaacaggat cggcttgttg gccaccggga ccagctgctt ggcgctggtg tgggtgatcg
     1321 gccgcaggcg cgtgccggct ccgccggaaa ggatgaggcc cttcacggcc cggtttctag
     1381 catgggggcc cgccgcgatc ggaaccgtgc gacgcccccg gggcccggcc gacgatccga
     1441 cgaacgggtc ccgggctcac tgggcggcga tcagggcgaa cgccagggcg ccggaccaca
     1501 ccacggcgac cgctgcgaga gcgtggggcc cgtgacgcca gcgtccgacg gtcgcggcgc
     1561 ccaccaccac cacgccgggg atcaacgaca tgtagtaccg cacggggatg tagacgaaga
     1621 tcccctggag gatgaagttc accgcgatga gcgccggtcc tcccagcagc gtcgcgatca
     1681 gcaccgaccg ggccatcgcc gcggtgcggt ccgatctcag cgcagcggcg acggtgacca
     1741 ccagcgaggc gaacgcgatc accgcgctcg acacgacgat gaacggcagt cgcaggaacg
     1801 gggccaggta gttgctcgac tcccccgccg cgctgaactg acccgtcgac aggaacgact
     1861 cggcgaacgg accccacggg aacgcctcca ccgtgtaacg gtgcagcatc tggatgtcgt
     1921 cggggtccga gctcgcgagc cgggcctgaa ccaccagcat caccgcggac accacaccgg
     1981 caccgagcag catgggcagc acggccgaga ccgagcggga caccgccggg ccgggacgca
     2041 gacgatcctg cgcgtccggc acgtcggagt cggcacgtgc cggaccgatg ctgaggtcca
     2101 cccgcatcca ggccccggcg atgaggacca ccaggttgga cggcttgagc gccatcgacg
     2161 ccgccgcggc caacccgtcg acgaccctgc cgccggggcg ggtccagcgt ccggcgagca
     2221 gcacggggac gcacagcacg agcgcaccgc cgagcagcgc ggtggcgtcg gggttgacgg
     2281 tggaggtggc gtgcaggacc gtcgggaacg tgaccgccag gatccccgcc gcgatgcggg
     2341 cgacgatccc ggcaccgagc cggcgcatgg tgggcaccat cagcagcagc gccggcagga
     2401 cccacatcgg gcccagcagc cgcgccgcct tgaacaggtt gtcgatcggc gtggccttca
     2461 cgaccgcctt cccgacccag gcggtgccga agtagtagac gggcgagtgg atgtaggcgg
     2521 tgttctggcc accctcctgg aactgcgacg cgtggtagct gccgatcggc ttgcacggca
     2581 tctcgaaccc gggggcgtcc accccccggc acgcctcctc gtgcatggcc tcgtcgccga
     2641 ccaggtcgcc ccggcccacc atctgaccct tgccggcctt gtagaggtag tccacgtgct
     2701 ggaactcgtc caccggcgac agcgtctggt aggcgccgac gtgcagccag ttgaacgcca
     2761 cgacgaccac catgaccagg gcgacgacga gggcgtcccc ccgccaggag tcctgccacc
     2821 ccgcctcggc ggccgaccga tcgctcgccg gaggaccgct gatcacgcgg cgatgtctag
     2881 catgcgaccg catcgccgac ccgggtcgcc gttgcccgcc gccgaatcct ccagcaccca
     2941 cccctccagc acagaccccc tcccgacagg gcccgaaccg tgaccgatcc gtacgtccac
     3001 cccaccgccg tcgtcgaaaa cggagcgatc ctgggcgccg gagcccgggt ctggcaccac
     3061 gcccacgtcc gggccggcgc ccggatcggg gccggcacca acctgggcaa gaacgtgttc
     3121 gtcgactccg gtgcggtcat cggcgaccgg gtgaaggtgc agaacaacgt gtcggtctac
     3181 acgggggtga ccgtgggaga cgacgtgttc gtcggtccga gcgcggtgtt caccaacgac
     3241 ctggttcccc gggcgttcgc cgaggactgg gtggtgaccc ccaccatcgt cggccacggc
     3301 gcgtcgatcg gcgcgaacgc gacgatcgtg tgcggcaacg acatcggcga gttcgccatg
     3361 gtcggcgccg gatcggtggt cacccggtcc gtccgcccgc accagctggt ggtcggcaac
     3421 ccggcacgtc acgccggctg ggtcgaccgg cgcggccacg tcgtgtcccg cgacgacgag
     3481 cctccaccgg cggagctgct cggacgctga gcggtcctgg gcgctgagcg gtcctggggt
     3541 cggtcggggt cccggaccgc tgcggtcact tgaccacgac gttgcgacaa cccaggttct
     3601 ggtccgcacc gccgccgacg ttgagcgcca ccgcgcacac ggtgtgggca ccgtcgggca
     3661 gggcccacag ctcggcaccc cacccgctgt tggagcccat ccccttggac gcggcgacgt
     3721 cgggtcgact ccgatcggca gggaccacgg cgacccggcg gccgtcgaag aggacagcga
     3781 ccgagatcgg ggcgtgggac tccggatcga acgcccagcc cgacagggcg acgtggcgac
     3841 cggaacgggt caccgactcg aggttgccca tcggcgacca cccgaggacg tccacgaacg
     3901 cgcagtccgc caccacgtcc tggccaccgc cgcggttgcc gacgatgacg cacacctcgc
     3961 gacggccgcc gagaacgccg gcccgcgtca cgtcgagccg accgtgggaa tcggtggtgg
     4021 tggtgagcac cggggacccg tcgacgtaca cgacgatcga tcgcggcgcg gcgccgtcgg
     4081 agtccgcggt gctcgcctgg acccggaaac caccgatcaa gccgacgatc gaggtgatcg
     4141 agccggtcgg gctgagtccg gtcgggtcat cgacgagcac ccgtcggcat cccagcgacg
     4201 tcggcggccc gccatccgcg tcgtggacgg tcacgcatgc catctgcttg cccgggggca
     4261 cccccgccag cgtcgcctcg aacccggtcg agcttccgta ggacggatgg accgccgcca
     4321 cgtcgggtcg actgcggttc gcggtcaccg tcctcgacga cgctccgacc gtcaactcca
     4381 cctgcggtgc ggtcgccgtc gagaggtcct tggcccagcc ccgggccgtc accgtcccgc
     4441 cgccgccgtc gagcgagtcg agactgccca ccgggacgct ggtggtgcgg atccacgtcg
     4501 cccactcctg ggcgcgacgg cggatggagc cgagcgagtt ggcgatgctg cccgggcagc
     4561 tggtctggcc gacgtcaccg tggccgacca ctccgggcag gtagagcagc tgaccggccg
     4621 ggtacttggt ggaacctccg gaggtgaagg cgaccgtgcc ggcgggatcg gcgaggtgct
     4681 gggccatctt ccagccgatg accgacgaga cgctctcgag cgcggcggcg gtcggggtgg
     4741 cctgcgtgta gtcgccgatc accatgacgc ctaccgaacc ggtgttgaag cccatcgcgt
     4801 gtgcgccgat gaccggccgg tcgatgccgc cgccacggcc ctcccagacc gtgccgaact
     4861 tgtcgaccac gaagttgtag gcgatgtcgg accagcctcg accgtccatg tggaacgcct
     4921 ggatggagcg gaggatgccg ggcacgtccc ccgggccgta cgagttgccg ctgtcggagt
     4981 ggtggaccac cgcgagcttg accgtgctgg cgtagctggt cgacagcggg gcacgggcgc
     5041 cccagacgtc gcgggtccgg atcccgaacg gcggaggagt tgccgccccg gccaccgggg
     5101 tcgagtccgc ggtgacccga cggagctcgt cgcggacggt gacaaccttg gcgccgacgg
     5161 cgtcgccggc accgaggctg acctcgtagc cggttcccga cccgacccac aggggcgcgg
     5221 tgccggatcg gccggcctcg gcgctgccgg cgtcggggcc ctccccgtcg tcggtctcga
     5281 gcgcctgcca cctgcccagg ttcccgtcgg cgtcgcgcac gcggaccagc accggtgcgg
     5341 tcggctcgga gtcgaaggtg aatccgatgg cgctgaactc gccagcggag tcgtcggccg
     5401 cgaccacccg gtctgcaccg accggctctc cggtggcggc ccgggcggcg tcgatctgcg
     5461 ccgcggtcat ggcggtgacg gtcgggacgt cctccaccgc tcccaccgat tcggtctcga
     5521 agtggagcac cgggaggggg acgaccgaga ggagcagacc gaatccgaag acgacgaccg
     5581 agcggcggag gatccggccg tgggggcggc aggacatggt cgtcgagtct aggggtcgac
     5641 ccgtggaatg cctcgcggtc ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnncaggac atggtcgtcg agtctagggg tcgacccgtg gaatgcctcg cggtcccgga
     5821 cccgaccgac gggcaccgcc gcgacggtac gactcagcga cggtacgact cagcgaccgt
     5881 acttctcagc gacggtgagc ttcggtcgac acagcctccc gcagcgcggc gatcaccggc
     5941 tccgcctccc ccgggtcacc tgccgggcgg tcgacgagga cacgccgaga gggaccgccg
     6001 ccgaccgggg tgacctcgac ggcgagccga ccggcggtcg cgtccgggac gagccggagc
     6061 ccgatccggt cgtggtcggt caccgagatg gggccgtccc ggggtgggat cagaggtgcg
     6121 gccaccccga ggacgatggc cacctcggcc ggagtggcca cccgagcgcc gtcgtcggcg
     6181 acgaccggga gcggatcgag ctcggcgtgg tcctgcagca gcgatcgggc caggatccga
     6241 ccggcggtga ccgtcccctc gtcccagcca gcggtcggcc attccgccac cagcggtgtc
     6301 acgtccaggg ccaccgccag cggaccccac ggccgcggcc gtggaagaga cggtgcgggg
     6361 cggggctgga accgcccgac gacacggtcg gtgggccgca tcccggtcgg gccgaccacc
     6421 gggtggttgc ggcgacggac ctcgacggtg tggtcggcca cgatccgggc ccatgccgag
     6481 agcagctgca gcgacggcag gtgacgccgc ttgagccgac cggcgatgtt ggcccgcagc
     6541 gcggttcgga tctcgcgtcg gacctctcga cgcaccaccg tccgctgcgc gggggtcccg
     6601 ttccgctcgg cggtgagcca ccagttgcga cggtccaggt ggaagaagcc ccgggcccgg
     6661 ctgccccccg acgccccgaa ggcgtgctcg acccgcgatg cgggcaccgc cacgatccgc
     6721 cacccggccc gggtggcccg ccacgacagg tcggtgtcct cgtagtacgc gaacaggcgc
     6781 gggtcgaaca gaccgaccga atccagcatc cgggagcgca gcaggaccgc tcccccgcag
     6841 aagccgtcga ccgggaccgg gtcggcaccc atgccgtcga gcaggtccac gggtcgcccg
     6901 tagagacggt cgtagccctc gcagcgctcg ttgcgggcgg ttcccaggcc gttgatcaac
     6961 cgggtccgct cgaggcccga acgcaacgtc gctgtcccgg gccgcgcgct cgacccgacg
     7021 cggtccccga tgcgcacctc gacggccccc gtaccggtca gctccacctc cacgtccagg
     7081 tcgccggagc cggcggggat ccagatgcgg ccggccccgg cgttcaggcg gtacgtgatg
     7141 tcgagaggcc acgccgcgtc cgacaccccc tcgaagccgt cgaagcgcag cgcgccggtg
     7201 acgtccgcac cgtcgacgcg gaccgcgacc acgtctgccg ttccatcggc gtcgacctcg
     7261 acatcgacgg gaacgaagcc cggttcgagc accaacgccg atgacgccgc cccgatgtcg
     7321 gccgcggcac ccagcgcgtc gaccaggggt cgcaaccacc ccggcgacgg caccgcgtcg
     7381 ttgttgatca gggcgaccgc gtcgacgccg tcgcggtcgc gcatcgcccg gttgcagccc
     7441 tcggcgaagc ccaggttggc gccgttggcg accacccgaa cgtcggggaa ccagtccctc
     7501 aaccgctcga gggagccgtc gacgctgccg ttgtcgacga ggaccacctc gacgcgatcg
     7561 gccgggtact cggtcgcggc cagggcatcg aggcatcgac gcgtgtacca ggccgagttc
     7621 cagttgagga cgacgactct cacgtagggg tcgaggtcct ccgccaccgc gccacggtac
     7681 ttcgccggcg ggtggcggcc gggagcggct cccgtgtcgg gccggaggcg agcggtacgc
     7741 ttcgcccgtg cagggaggcg acgccgccgc ggcgctggac gcccaccccg accggcctcc
     7801 cgagccggac accgtcatcg gcgcggacct tccgggctcg gccggcctgg tcagcactcc
     7861 gctgccccgg ggtggggccg cgtggctccg cagcgccctg accggcggcc agtcgtctct
     7921 gaccctgggg ctcctgttcg acggggtcgc gtcgtacgtg ttcctgtccg ccgcggcgag
     7981 ggacctgggt ccctaccggt tcgccatggt gtcggtgctg tgggtcgtgc tgttcctgat
     8041 cggcaacggc ctgttcatcc cggtcgaaca ggagctgtcg cgttcgatcg ccgcccgcgc
     8101 cgtgcggggg tcgggctggg cgtcgttggt gcgccgcgtg accgtcgcct cgggcgccgt
     8161 gctggcggtc gccatcggga cggggatcct cgcccgtggt catctggccg actcgctgtt
     8221 ccggggcgac cagaccttcg tgtggctgct gctcggcggg ctcgtcggcg tgtggttgat
     8281 gttcgtgctg cgcggcgtgc tgtcgggcga gcgccgcttc cacgcctacg ggctgatgtt
     8341 cgtcgccgac gccacggcca aggcgctgcc cggcgtcggc ctgctgtggt ggggggcgct
     8401 gcgaccgtcg tcgtacgggc tggtggtggc cggcagcgcc tacgccggca tcgtggtcgc
     8461 ctacgcctgc ctccggtggc gacgagccca aggttccgcc tcgaccagcg acgtgctgac
     8521 ccccgatccc gaggcccccg acgtcgcagc tccgtggggt cggctgtcgt cgtcgatggg
     8581 gttcctgctg ctgacgtcgt tcctcagcgc gttggcgatc aacatcggga cggtggccat
     8641 cgaggtgacc ggcacccggg tggacgccga caaggccggc atcttcctgt cgggcctggt
     8701 gatcgcccga atcccgctgt tcctcttcca ggccatccag gcgatcgtgc tcccccggct
     8761 gtcgctgctc gcggcgcgtg gcgacctggg cgggttccga tccgacctgc gtcgcctgta
     8821 cgccgcgatg ggaacgacca ccgtcgtcgc caccctggga gccgccgcgc tcgggccgct
     8881 ggcggtgcgg gtcctcttcg gcgaggagtt cgccctgctc ggcgcccgcg acatggcgct
     8941 gctgaccctg tcctcgttgc tgatgacggt cgccctcacg ctcaaccagg cgcagatcgc
     9001 attgcaccac caacgccaga ccggctggcc gtggggcgtg gcgaccgccg cgttcctggc
     9061 catcgtgacg accaacggac gcgacgtgtt cctgcgggtc gagctgggca tggtcgcggc
     9121 cggagcggtc gcggcgttga tcgtgacgat cctggtccag cgggagctcc gccaccccga
     9181 ccacctccgc tccgacaccc cggcgctctg aggtccgatc ggcacgccat gggctcggct
     9241 ctggacgtgg tcgtcgtcgg tggcgggatc gtgggtctgg cgacggcgcg agcactgctc
     9301 ctcgatcgcc ccggctcggc agtcgtggta ctggagaagg agtcggcccc cgcacgtcac
     9361 cagagcggtc gcaacagcgg ggtgatccac tccgggatct actacccacc cgggtccctc
     9421 aaggcgctgc tctgcgccgc gggccgtcgg tcgatggagg cttacgcccg ggaccacggg
     9481 attccggtcg agatcaccgg gaagctgatc gtggcgaccc gcatcgagga gctcgatcgc
     9541 ctggacgcgc tggccgaacg gggccgggcc cacggcctca ccgtccggac gatgccagca
     9601 gccgaggcgg tggaggtgga gccgcacctc cggtgcgtcg gcgcgctgca cgtcgccgag
     9661 accggcgtcg tcgacttccc cgcggtgtgc gcgcagctgg cccgagacgt cgtcgagctc
     9721 ggcggggagg tccggaccag ctgtcgggtc accgctgtgg ccacggtccc cggcggtcaa
     9781 cgcgtgacga cgacgtccgg cgagctccgg acccgacgcc tggtgaactg cggtgggctc
     9841 cagtccgacc tgttggccgc ggcggccggc gccaacgtct ctgggcggat catcccgttc
     9901 cgcggcgagt atcgggagct ggtgccggcc gctcgcccat tggtgcgcgg cctcatctac
     9961 ccggtaccgg acccccgctt cccgttcctg ggcgtgcacc tgacccgtgg cgtcgacggt
    10021 ggggtgcacg ctggtccgaa cgcggtcctg gcactcgccc gcgagggcta ccgctggatc
    10081 gacgtcgaca tcggcgagac ggtggacacg ctgcgctcgc cggcgttccg gcacctcgcc
    10141 cgccgccatc tacgcgacgg gatcggcgag gtggtccgct cgttgtcgga gcgacggttc
    10201 ctggccgagc tgcagcggct ggtccccgag ctccgggccg aggacctgca acccgctccg
    10261 gcgggggtgc gggcgcaggc ggtgctcgac gacggcacgc tggtcgacga cttcctgctg
    10321 gtgtcggggc cgggggcgct gcacgtgctg aacgctccgt cacccgcggc gacggcgtcg
    10381 ctcgagatcg gcgccgagat cgccggccgc ctcaccaccc cctgacccgc acctgacccg
    10441 ttctgttcgt tggatcacaa ccgtcaggtt gtgatccaac gaacagaacg gggggtgggg
    10501 tgcgtttggg gatcaggacg ggggctggag cggggcgggc tggcacgccc cctcgagctc
    10561 ggtcaccacg atctggtccc ggttctccca cgtgatcggg ttcgacgggt ccttgcggat
    10621 cttgaactcc cggaacgtca tgtcgagcgc gtcgaacgcc aggtaccgcc cgatcagcgg
    10681 gtcgccgatg cccaggatca gcttgagcgc ctcgagcgcc tgcagagtgc cgacgatgcc
    10741 gggcagcacg ccgagcacgc cggcctccga gcagctcggg gccagctctg cgggaggcgg
    10801 ctcggggagc atgtcgcggt aggtcggtcc gttcttcggg tcgaagaccg tgacgttgcc
    10861 ctcgaagcgg aagatcgagc cgtgcacgac ggggacaccc agcttcaccg aggcgtcgtt
    10921 gagcatgtag cgaaccggga agttgtcggc cccgtcgacc accaggtcga actgcccgac
    10981 gatgtcggcg atgttcgagg cgtcgaggcg cacgtcgaag gtgatgacgt cgacgtcggg
    11041 gttgagggcg gtcagggtct tcttggccga gtcgaccttg cgctcgccga tccggtcgac
    11101 gttgtggagg atctggcgct gcaggttcga gtcgtcgacg acgtccatgt cgacgatgcc
    11161 gagggtgccg acaccggccg cggccaggta cagcgccgcg ggcgatccga gcccgcccgc
    11221 gccgagcagc aggacccggg caccgagcag cttcttctga ccctcctcgc ccacctcggg
    11281 caacagcagg tgccggctgt agcggttgcg ctgctcgggg ttcaggacct cgggcacccg
    11341 ccagttgcgg ccctcgtcct tccagcggtt gaagccgccg gccatcgaca cgacgtcggt
    11401 gtaaccgagc tcgcccagcg tccgggccgc gaacgcggag cggaccccgc cggcgcacat
    11461 gaccacgacc gggccggcct tgtcgccgag gcggccctcg acctggctct cgaggtgtcc
    11521 ccggggcagg aacaccgatc ccggaacggc cccctgctcg aactcgtcgg cctcgcggac
    11581 gtcgagcagc gtcgccccgc cgtcgatcat ctcctcggcc tcggcggtgg agacctctcg
    11641 gatctcggcc ttggccgcgt tgaggaggtc gcggaagttc gccatcgtca tgctccagag
    11701 tcggatcgtc ggccgccggt cggcggcgtc accgggtaaa accctaccca actggtcaac
    11761 attcccgaaa ggcgggacgt ccggtcgggt cgagtcgact cagccgagga tggcgggcag
    11821 cccggccacc agcgcgggaa gcagctccga gaggctcccc cgcagcacaa cggtggccat
    11881 gtcgtccatc tcggtctccg agccgttgac gatcacgacc ggagctcccc ggtcgacgac
    11941 gatcggcacc atgtgggcga cggggtacac cgccagcgtg gtgccgaccg cgagcagcat
    12001 gtccgcctca cccgccgcgt cgagcgcccg ggcgatgtcg gcctccacca gggcctcgcc
    12061 gaagaacacg acgcggggct tgatgacccc accgcagtcg ggacacgccg gatcgagctc
    12121 accggagcgc acccggtcga acacgtcacg cgtcgggccg gaccagtcgc agcgcacgca
    12181 gtgcgactcg ggcaacgacc catggacctg cacgacgtca tgggggtcgt ggccggcggc
    12241 caggtgcagt ccgtcgatgt tctgggtgac caggaggtgc atccggccct ggaggtccag
    12301 gccacgcagc gcccggtgcc cggcattggg ctccgcggtc cacgccgggt ggtccatccg
    12361 gcgacgccac gcccgctctc gcacgctgcg ctcggccagg tacacgtcga tggtggccag
    12421 gcgctcggct tcggggtccc gggtccacac cccgtcggga ccgcggaagt cgggaatgcc
    12481 cgagtcggtc gagatgcccg cgccgctcag caccacgacc cgacgcgacc cggccagcag
    12541 gtcccgggcg ctctccagtt cccgcgctcc gtcgggcatc gggcgatcct acggccacgc
    12601 ctcctggacc gcgtcggcga cgtcgtcgag gtcgagtcgg acctcgtcga agcggcgggt
    12661 gtcgcggaag gtccggtccc gaccacccgg gtcgacgaac cagccccgca cccgttcacg
    12721 cacggcgtcg gccaggaccc cgggggcacc tgcgagctct tcggcccacc ccccggtcga
    12781 tccgagcaac cggcccaggt cgtacaggtc gctggcgcgc ttctccggct tggagctggt
    12841 ccgcaggggg atcgtggtcg ccttcatcgc gaccaggccg ccgggactgg cgacgagccg
    12901 atcgggagcg gatgccagca cggtgccgtc gagctcggac acgacgacca ggtccagccc
    12961 ggtggcgacg tcgtgggccc aggcgtgggc gaccgcgttg acctccaggt cggtcagcgg
    13021 ctcccccgcc gcgacgagct cggcgaccag gtcgtgggcg tccgcatcgc cgacgtcgat
    13081 cacgtcgagc tccaggcggc cctccatcag gtactgaccg ccgcccgggg ccgacagcgc
    13141 cacggagcgc agccccgggt cgaaggagtc gaggccccgg gcgagcgcgt cgaggtccac
    13201 cgtcgcccgg tgcgggacac ccacccggca cagcacggcc agacctccga tcaggcggaa
    13261 ctccgcgggc agaccggcga gcacctcgac cgaccgggcg gtggtgccgt cgggccggcc
    13321 ggtcaggacc accgatccgg tcacggcgcg tcccccgatg cggcggcgcc cgggacgaca
    13381 ccccagtcct cgacgatctc gcgacctcgg gcgggatcac gggcgaggcg gagggcgcac
    13441 accatcggtg cggcgacgtg ccaggggtgg tcgtcgtcgg ggccgaaccc ctcgagctcc
    13501 ggcaaccagc ccaccggcga catacgcaga aaggtgcggg gcgccccgga ggaaccgaac
    13561 gccgagcgga cccgccgcag agcgccgcgg gtgtgcacgt agcaccgagc gggcagcgac
    13621 gccgccgccg cgatgcgcgc cccgcccagg gtggcggcgc gctcgtcgac acggaccagc
    13681 gcgtcggcac cgaggacggc gacgacgtcg ggcagctccg cggccacggc ggtccagtcg
    13741 tcgtcgggcc agtgagcggc ggtctcccag aacaggtccg gcagcagggg ccgccggctg
    13801 gtgcgaccga tcaacccgtg ctcggcgaac cgcccgatca cctcctgggt gccaccgacg
    13861 ctccggccga tctccgcggc gacggcccgg gcacggaccg cgtcgtcggg gtggcacagc
    13921 gcgtggaggg cgacctcgag gccgacggcg gtccacggcg acgacctcgt tccccgtccg
    13981 tcggaaccgc cgagggcgac gtcggtctcc agccgcaggc cgggtcgcca cacccggagg
    14041 tgccctcgac ggtcgagcca gctccacccc gcggcacgca gcacgtcgcg acccgcggcg
    14101 ctgatccgat ccgccaccac caggcccggc tcgacggtct cccgggattc gacgagggcc
    14161 gccagctcac ccgggtgcgg atggctgcgc tcgatgacgt cgatgtcgag gacgacgccg
    14221 tcaaccgtca ggcgccctgc cggcgtcgtg acgtccaccc ccgcggcccg gcacgcctcg
    14281 atcaactccc gcccgatagg tgtacgtgac gtcacgaaca tcgaacacac cctatcggac
    14341 gaactgccgg cagcgacctg gccttagcta ggctaggacc atgtcgacac tggtcgtgaa
    14401 cacccacgag gccaagtccc ggttgtccgc tctcatccgg gccgccgagg agggtgacga
    14461 ggtgatcctg gcccgcaacg gcagacccgt ggcccgactc gtgccatgga ccacctccgc
    14521 ccccacccga agccccgggg cgtggtctgg acggttcgag gttcccgacg acgtcgtcgg
    14581 gtccgatccc gacgtgatcg agatgttcga ccgctcggcg gacagcaccc tgtgagacga
    14641 ctcctcatcg acagccacgt cgcgctctgg tggctcagcg gcgccgcggc gctggggccg
    14701 accagccgag ccgctctgac cgacgcggat gtagtcctgt actcggccgt cactccgtgg
    14761 gagctgggga tcaaggagtc actcggcaag ctcaccctgc ccgaggacct cgtcgacgcc
    14821 ctcacgaaca ccggattcac cccgctcgac atcactgccg accatgcccg gcgcgccgcg
    14881 gcgcttcctc cgcaccatcg cgacccgttc gaccggatgc tcatcgccca ggcccagctc
    14941 gagtcgctcg tgatcgtgac cgcagacggc tcgttcggcg actacgacgt cgatcggctc
    15001 gacgcccgca cctgactcca ccagggatcc gatcccgacg cggtcgaggg tgcctccagg
    15061 gacggtgccg ccggcaaatg gaacaggtgt ccgtgacggc acgaacggcg aacaccgggt
    15121 cgcgctcccc gttgcccacc gccacccgat ggcgcgaccg tgtgtctgcg gtcgctgcgg
    15181 cggtcgcccc acttccccct cccgcctcgt cggatctccg caacgccgga ccgacgcgcc
    15241 gtcaccagga gtggaacgtg acccgcaccg ccgtcgacct gcctccccac cgcggatcgg
    15301 gaccgatcga cccgtccccg ggcgaacctc ccgaacggct caccgtgaag ccgtggtggg
    15361 acagccgtct ggcgctggtc ggcgtcaggc cggacgaccc ctacgtggag aggttctggc
    15421 tggcgatcgt cggcccgagc accgtggtcc tgctccgtcg gttcacccgc ggcctcgacg
    15481 aacaccccga gggcttccgg gtggggctcc tcgagacgtc cctggccctg ggactcggac
    15541 ggggcacggg ccgcaacgct ccgatcagcc gcacgctcgc ccgggccgag acgttcgggc
    15601 tgatgcgacg gaccggcgag cacggcctcc aagtccgcac ccatctcccc gtgctgagcg
    15661 cccgccaagt ccgccagctc ccgccggtgc tgcgtcgggt ccatgccgac tgggtacgga
    15721 tccaccggac ggaccgaccg tgaccgacgt ccgcgccact ccgcccggct ccgcgccgac
    15781 cggctccgaa ccgccttctg tagcacctgg tgctacagtt ggtgcggtga ccggcgagat
    15841 cagccagcgg gagctccgca acgacagcgg acggatcatg cgcgccctca ccgagggcga
    15901 gtcgttcatc atcacccgca acggcgaacc catcggcgag ctcacgcctc ggcgcaggcg
    15961 gcgcttcgtc cgtgccacag ccgtggtcga gctcttccga tccgctccgc cggtcgacct
    16021 cgacacactg cgccgcgacc tcgacgccct cgcaccccag gacgccgtgc cacgtgggtg
    16081 agaaccgtca cgaacgcgga gtcatcgaca cgtcggtcgt catcgacctc gaatcgatcg
    16141 acgcgtccgc tctgccacgg gaggtcgcca tcagcagcgt caccctcgcc gaactcgcgg
    16201 ccggccccca cgccaccgac gatccagccg agcgggcccg acgtcaggac cggctccagc
    16261 gcgtggaagc agcattcgat ccgctgccgt tcgacctcga ggcagcgcgc gcctacggca
    16321 gggtgttcgc agcggtcgcg ggcgcggggc gtacgccgcg gggcgcacga gcggtcgacc
    16381 tactcatcgc cgccaccgcg tgcgccgagg ggatgcccct ctacacccgg aaccctcggg
    16441 acttccgggc gctcgacgga ctggtcgacg tgatcgcggt ctgacgccac cgccgacgcc
    16501 cacagtaccc agccgtgatg gtccgggaga cgtcaggccg agtccggatc gcgggggaac
    16561 gcgaccccac ggcccaggac ctcgagagcc aaccggtcgc cgaccgagac gttaccgagc
    16621 gacgacggcc agcgggccgt cacatcgacg cccgccacct cgagcgtcac caccatctcc
    16681 gcgccggtga agcggacccg acgcacccgg ccgaccggcg cctccccgac cgggccgcgg
    16741 tcgccgggga gatgccgcca cacctgctcc ggtcgcagga acacccggac ccgcccacgc
    16801 ggggacggac cgttgcgggc gacgtccaca tcgaggtcac cgagcacgca ccggacccgg
    16861 gcaccaccgc caccgtggcc cagcagctca ccgtcgagca gcacagcgtc tcccacgaag
    16921 cccgccaccc actcgtcggc cggcgaccgg tacacctcgg tcggcgttgc gtcctgcacg
    16981 acacgcccgt cgcgcagcac cacgacccgg tcggccatgc tcaacgcctc ctcctggtcg
    17041 tgggtgacga ggatggcggt ggtcgccgcg tcgctcagaa cccgggcgac ctcggcgcgc
    17101 agcgacgacc gcagcgacgt gtccagcgcg ctgaacggct cgtcgagcag cacgacgtcg
    17161 ggtgccgggg caagcgcccg ggccagggcg acccgctgct gttggccgcc ggagagctcg
    17221 tcgggtcggc gggaggacaa cgacgccagc ccgaccagct cgagcatctc cgccacccgc
    17281 tcgcgccgct gggacctcgg tcgtcccgac agtccgaacg cgacgttgcc ggccacgtcg
    17341 agatgcggga acagggtgcc gtcctggggg acgagcccga cccgacggtg ctcgggcggc
    17401 acgtggcgtc cgggcgcgtc cacctcacgg cccccgacgt ggaccgtgcc cgagtcgggg
    17461 cgcaccgttc cggccaggac gcccagcagc gtcgacttgc cgctgccgga cggtccgagg
    17521 accgcggtga gcgacccggc ggcaacggcc aggtcgaccc cgtcgagcgc gacggcgggc
    17581 gggtccgcgg cgaaggtccg cgacacgccc tcgagggcca cgacggatcc ggcggcggga
    17641 ccaccgggtc gggccccctc ggcgtcaccg gaccggcgct caggcgtcgt cggtgtcctc
    17701 gtcgccatcg tcgtccgggg agtcaccggc tccgccggcg tcccagccct cgagaagggc
    17761 gagaagggtc cggacaccga agccggtcgc ccccttgggc tgctcgccgt cgggctcctg
    17821 caggaacgcc ggaccagcga tgtcgaggtg cgcccatggg atcccgtcgc cgacgaagtg
    17881 ccgcaggaac aaccccgcgg tcagcgtccc tccaccggtc tgggaggtga tgttcttgat
    17941 gtcggccacg gcgctgtcga ggcgggggcg gtagtcctcg ggcaacggca gcggccacac
    18001 ccgctcccct gcggtcgcgg cggcctccgc gacggtggcg cggaagtcgt cgtcgttgga
    18061 catcagaccc gcgatgcggt cccccagggc gaccatgcat gcaccggtca gggtggcaag
    18121 gtcgacgatc gcgtccggct cccgctccga ggccaggatc agcgcctcgc cgaggaccag
    18181 ccgaccctcg gcatcggtgt tgagcacctc gacggtcgtg ccgttgcggg cggtgtagac
    18241 gtcgcccgga cgctgggcgc ctccgttgat catgttgtcg gtcatcgggg tgatgccgac
    18301 cacgcgcacc ccgactccca gcgccggcag cgcgcacatc gcggagatca ccgccgccgc
    18361 gccgcccatg tcgcacttca tgtccatcat cgacgcggcc ggcttcaggg acaggccacc
    18421 ggagtcgaag gtgatgccct tgccgaccag ggcgacggtc ggggtgtcgt catcggcccc
    18481 gtcgggttcg taggtgaggg tgaccagtcg gggttcgatc gtcgatcccc ggttgacggc
    18541 gatcagcccg cccaggccgg cctcggcgat ggccgccagg tcgaggacct cgacaccgat
    18601 gcccgccgcc gtggcgcgga ccgcggcccg atccgcgagc tccggggcgg tcagggtgcc
    18661 accgggcgtg ttgaccaggt cgcgggcgaa gcagacggcg tcggcgaccg cggcccctgc
    18721 ccgcaccccg tcgcgggccg aacgcaccgc ggcgttgtcg tcgccgacca cgagggtcgc
    18781 ggtcgccagg cccggcttga cgttcgcggc ggagcgcagg tcgtcgaagc ggtaggaccc
    18841 cagcaccagc ccctcggtca gggcccgggc aactgcggcg aggtcatcga ccgccgacgg
    18901 gtcggggacc tcgaacgcga cccgacggtg ccgtcccacc gcccgggcga acgcggcggc
    18961 accgcggcgc agggcgtcgg tacgggccga accgctcgtc gtggacggac cgagcccgac
    19021 cacgacggtg gcccgctccc cgtcgtcgaa ggtggcggtg gtgcccacgg caccggagaa
    19081 gcccgcccgc tccagggccg ccttgcgcag gccgggcacc ctgttcagcc ggtcgctgtg
    19141 ggcggtgacc gccaccgcgg tggcccccga cggggtggac ttggcgacct tgacgtcgac
    19201 gggttcggtc atcttcagct cctggtgctg gtcacggacg gggtgatgcg ttggggatga
    19261 ggtgagggga tcggcggact cccggcggat cgcggcgggt cacgaggagc cgtcgttcac
    19321 cgaacgggtg gtcagcgatc ggccctcctg ccagcgggcg agcgcccaca gcaggtccga
    19381 cagccggttg aggtacggca ccacctggga gccgtcctcg gccgcggcca gggcgctgcg
    19441 ctcggcgcgc cgcacgacgg ttcgggccac gtcgaggtgg gcggccacca cggtctcgcc
    19501 gggcacgacg aactcgcgcg gggcctcgaa gcggcccatg acgtcgtcga tggtggcctc
    19561 gaggtcgctc accatcgact cggtgaccag ggacgtgccg gcgctcagct tgtggcggtt
    19621 gcccgacgcg gtggccagct cggacatggc cacccacagg tcgcgttcga tccggacgag
    19681 cagctgcacg agctcgtccg cggtgtcggc gacgtgggcg ccgtcgggca ccgcggccgc
    19741 ggcgtgcgcc cgggccacac cgatggcgga ctgggcctcg tcgacgtcgc cgtaggcggc
    19801 gggcgccgga tcgtccttgc ggacccgacc tccgtaaagg agcccggtcg tgccgtcgtc
    19861 gcccttgcgc gtgtagatgt ccatccgccg gggagcctac cgacggcgcc ggcggctggg
    19921 gagcgtcacg ggggccgcaa gtagcatcgg ttgcacctat ggctgacact gctccgagct
    19981 cgcccgcgtc cccctacctg gccgcgatcg cggaccacgt cgtcgtcttc gacggcgccg
    20041 cggggacgtg gttgcaggaa caggacctga ccgtggagga ctacggcacg ccggagctcg
    20101 agggctgccc cgagatcctc gtcgagaccc gtccggagct gatcgcccgt atgcacgccg
    20161 agtacctcga ggccggcgcc gaggtcatcg agaccaacac gttcgggggc atgcgggcca
    20221 cgctcggcga gtacgggctc ggcgatcgca ccgtcgagct caaccggatg gccgcagccc
    20281 tggcccgcga ggtcgccgac cggcactcca ctcccgagcg gccccggttc gtcgccggct
    20341 cgatcgggcc gggcacccgg ttcgcgtcgc tcggccaggt ccgctacgcc gagcttcgcg
    20401 accaggtcgc cgaacaggcc cgcggcctgc tcgagggggg cgtcgacctg ctcctgatcg
    20461 agacccagtt cgacctgctc ggcatcaagg cggccatgaa cgggtgccgg gccgcgatgg
    20521 aggaggtcgg ccgcaccgtc ccgatccaga cccaggtcac gatcgagctg accggccgca
    20581 tgctgccggg caccgagatc ggcgccgcgc tggtcgccct cgacgccatg caccccgacg
    20641 tgatcggcat caactgcgcg acgggacccg aggagatggg cgagcacgtc cggcacctcg
    20701 cccagtactc gcgcatcccc atctccgtgc tcccgaacgc cgggcttccg tcggtggtcg
    20761 acggccacat gcactacgac ctgaccgccg aggacttcgt cgcccaccag cgccggttcg
    20821 tcgaggagtt cggcgtcgag gtggtcggcg gctgctgcgg aaccactccg gagtacatcc
    20881 gccagctcgc cgagatggtc gcctccacac cccgcgccga gcgcaccccg accttcgagc
    20941 cgggcctgag ctcgatctac tcccccgtca tcctctcccc cgccgaggac gacgccgccg
    21001 gatccgcgtc gaccaacctg atcatgatcg gcgagcgcac caacgccaac ggctccaaga
    21061 agttccgtga ggccatgctg gccggcgact gggacacctg cgtacagatg gccaaggacc
    21121 aggtgaagga agggtccgcc gtgctcgacg tctgcgtcga ctacgtcggc cgcgacggca
    21181 ccgccgacat ggacgagatc gcccagcgct tcgccaccca gtcctcggtg ccgctggtgc
    21241 tggactccac cgagcctccc gtgctcgagg ccggcctgca gtgggtcggg gggcgggcgg
    21301 tgctcaactc cgccaacctg gaggacggcg agggtcccgg atcgcgcttc gaccgggtca
    21361 tgaccctggc ccgcacctac ggcgcggcgg tgatctgcct gctgatcgac gaggagggcc
    21421 aggcccgcga cgtcgagtgg aagctgcggg tcgcccgccg gatccacgac ctggcgatcg
    21481 agcgctacgg cctgggagcg tccgacctgg tcttcgacgc cctcaccttc ccgctgtcca
    21541 ccggcgacga cgacctgcgc ggcgatgcca tggccacgat cgaggcgatc cggcgcatca
    21601 aggccgagct gccgggggtc cacaccaccc tcgggctctc caacgtcagc ttcgggctct
    21661 cccccgctgc gcgccacgtg ctcaacagcg tgttcctgca cgagtgcgtg caggccgggc
    21721 tcgactccgc catcgtccac gcgtccaaga tcctgcccct caaccggatc cccgacgagc
    21781 agcgcgacgt ctgcctggac ctgatctggg atcgccgtgg aaccgccggc gcgctctccg
    21841 acggcgacga ctcctatgac ccgctggccc gcctgctgga ggtgttctcc gacgtgcagg
    21901 tggccgaggc cgtcaccgag gaccgctccg gttggaccgt cgagcagcgc ctgtcgcagc
    21961 gcatcatcga cggcgaccgc gacggcctgg tcgacgacct cgacgaggcg ctggcggccg
    22021 gtctgtccgc gttggcgatc atcaacgacg tgctcctcgc ggggatgaag gtcgtcggcg
    22081 agctgttcgc caccggcgag atgcagttgc cgttcgtgct gcagtcggcg gagaccatga
    22141 aggcgtcggt cagccacctc gagcagttca tggagaaggc cgacggcgac gccggcaagg
    22201 gtcggatcgt gctgggcacc gtcaagggcg acgtgcacga catcggcaag aacctggtcg
    22261 acatcatcct caccaacaac ggctacgagg tgcacaacct cggcatcaag atctcgatcg
    22321 ccgagatgat cgacaaggcc ctcgaggtcg acgcgcacgc catcggcatg tccggcctgc
    22381 tggtgaaatc cacgttgatc atgcgcgaca acctcgagga gctcaacgag cgggggctgg
    22441 ccgagatccc ggtcctcctc ggcggcgccg cgctcacccg cacctacgtc gagcgcgacc
    22501 tgcgcgaggt ctacgaggga cgactcttct acggcaagga cgccttcgag ggtctgcggg
    22561 tcatggaccg cctcggcgac atccgccgcg gcggagagga cgacccgggc tggggccgcg
    22621 agccgggcgg acgtgacatc ccggcccgtc gggtgcaccg cgacgacgag cccgaggtcg
    22681 agctgccgga gcggtccccc gaggtgatga ccgacaaccc ggtcttcgtg ccgccgttca
    22741 ccggctcgaa ggtgatcaag gggatcccgg tcgacgacgt ggccgcgttc gtcaacctga
    22801 ccggcctgta ccgcaaccag tggcagttcc gccccgacaa gcgcgccgac gggtcggtcg
    22861 agaccgacga cgagttcaag gagcggatgc ggccgatgtt ccgcgagcag ctcgcggcgg
    22921 ccaagtcgga ggggctgctg gtcccgcagg tggtgtacgg cttcttcccc gccaacgcgg
    22981 atggcaccga cctggtcgtg tggaccgacg agtcccgctc ggaggaacgg gcccgcttcc
    23041 cgttcccccg ccagcgcaag gagccgttcc tgtgcatcgc ggacttcttc cggccggtcg
    23101 actccggcga tctcgactac gtggcgttcc agatcgtgtc gatgggctcg gcggtctcgg
    23161 agcgcaccgc cgagctgttc gcggcgaaca agtaccagga ctacctgttc ctgcacggcc
    23221 tcggcgtgga gatggcagag gcgctcgccg agtactggca ccggcgcatc cgccaggagt
    23281 ggggattcgc ggacgaggac ccacccggcg ccgacgcggc cgcccccgac aacggtgctc
    23341 tcaacgggct gttccgacag cggtaccgca gcggccgcta ctcctgggga tatccggcgt
    23401 gcccggacct cgaggacaac cagaccgtgg ccgagctgct cggcgccgag cgcatcgggg
    23461 tcacctgcga cgaggacacc gggttccagt accacccgga gcagacgacc tcggcgctga
    23521 tctgccacca cccccgggcc aagtacttcg tcgcccgctg agcggctact tgacctgggc
    23581 ccgtcgcatg aagccgagcg ccgggtagac cgcctgcggg gtgatgcggt cgtcgtagac
    23641 gctgccgccg atcgccccgg tgtcgctcac cgcgatggcc atccgggcgg cgtcgtcgac
    23701 ggtcatgccg gtcccctcgc cgccgatcgg gtgcacgggc aggttcgggc gaccggtgag
    23761 ggttcgcagc cgcgtcaccg actcggcggt gtacaggtac gggtcacccc actcggggaa
    23821 gctgccggca cgccggtagc tccagtacgc catcggcatc caggcgtcca tgtagcgcga
    23881 cagctcccgg tacgggaagt tcggccacca cgatcggttc agctgctccc agatgatcgg
    23941 agtgacgggg atggctgcca tcgggacgtc ggggtgcagg gcccgcagga accgcacctc
    24001 ggccatgagc ttgtcggtgc ggtccgcgac gtccgggttg tcggtggcct cgatgtcgat
    24061 gccgatgccg tcgacgccga gtccggtgat ggccgacatg cgacggatgt cgacctccat
    24121 gtccaggaac tgcggcaggt accagccgac caccttcatg ttccgcaggt gcgcccgccg
    24181 gacgatggac cgcagcagcg tggggtcgag cacgtcgtcg gggtgacggt aggtcgcggt
    24241 ctggatgtag agggtctgga cccccgcggc ggcgagtcgg tcgacgtcgg ccaggtcctg
    24301 cggcgccgag cccccggtga aggtgggcga ccagtcccag acgtcccacc acgcacctag
    24361 tcccttgaac gggatcgtcc ggaccggatc ggggccgttc caggtccgtc cggcgcccgg
    24421 catgcagccc gcgagggcga cggcgatcag ggtcagggtg gcgaacaggg cgaggccgcg
    24481 acgacggagc accggtccac gctatcggcg ccggagcagg gttccgcccc gggagcgggt
    24541 gaacgctccg accccgggga ccgtcccgct tgggtacggt gcgcccatcg tgacgtgggt
    24601 gcggacacgt cgatgacgga tcgggggacg aacgatgagc acggtggaca ggacccggct
    24661 cgcgtcgctg atggcggccg agcacgagcg cttcgtgagc cgcactcccc gatcggcgga
    24721 gctgcaccgc caggcccgca ggtccctgtt ggccggggtc ccgatgaact ggatgacccg
    24781 gtggccgggt ccgcatccgg tcttcgtgga tgacgcgtcg ggtgcccggt tcaccgacgt
    24841 cgacggcaac acctacatcg acctgtgcct gggcgacacc ggggcgatga ccggacacgc
    24901 cctgccggcg acggtgcggg cgatcgagca gcaggcaccc cggggcatca cgaccatgct
    24961 cccgtcggcc gacgccgtcg ccgtcggcga cgagctccgc tcccgtttcg gtctgccggt
    25021 ctggcagatc gccatgaccg cgaccgacgc caaccgcttc gccctgcgga tcgcccgcca
    25081 cgtcaccggc cgccggaagg tcctggtgtt cgactggtgc taccacggca ccgtcgacga
    25141 ggccctcaac gtgctcgacg gcgaccgggt ggtcccgcga ccgggcaaca ccggttgggc
    25201 gaccgacccc gagctgtcgg tggaggtcgt ccagttcaac gacgtccccg ccctggaacg
    25261 ggcgctgtcg accggtgaga tcgccgcggt gctggccgaa cccgccctga ccaacatcgg
    25321 catcgtcgcg ccagatccgg gcttccacga cgccctccgt cgcctgacct ccgacaacgg
    25381 gacggtgttg atcatcgacg agacccacac gatctgctgc gggcccggtg gagcgacgcg
    25441 ggagtggggg ttggagcccg acatgttcgt catcggcaag ccgatcggcg gcggcgtgcc
    25501 ctgcgcggcg tacgggatga ccaccgacat cgccgagcgg gtgatcgacc tggtcgacgg
    25561 gcccgacagc gacgtggccg gcatcggggg aaccctcacc gccaactcgt tgtcgctcgc
    25621 cgcggtgcgc gccacgctgg cccacgccct gcgcgacgag gacttcgccg tggcggtccc
    25681 gctggccgag gcgtgggccg acggcgtgcg ctcggtgatc gaccgccacg gtctgccgtg
    25741 gacggtgaac cggctggggt gccgggccga gtactggttc tgccccgccc cgcgcaacgg
    25801 agccgatgcc gccgccgcgg tcgatcacga cctcgacgcg ttcctgcacc tgtggtgcct
    25861 gaaccgaggg atcctgctga cgccgttcca caacatggcc ctgttctgcc cggcccacac
    25921 catctcggac gtcgagcacc acaccgccgt gttcgccgac gccgtcgcgg cgctgaccgc
    25981 ctgaggtcga gacccccgtc cgccgatcag cttcgggggg ccggcaggcg ctggaccgcc
    26041 tccagggcgg tggcgaacag gtcgccgtgc tcggccaccc gctccagcac gtctccgatg
    26101 cggaacacga gcggatccga gccgtcggcg gccgcggcga cctcggccca gtgcagcggg
    26161 gtggacacgg tcggttcggg tcgggcccgc atcgagtaca cgcacacggt cgtcttgtgg
    26221 agcgcgttct gggaccagtc gacgaacacc ttccccgacc gctgcgcccg ggccatggtc
    26281 gtggtcaccc ctcgggggtg gcgctgggcc agcagctggc cgacggccag ggcgaacgac
    26341 gcgcaatgct cgtggtccgc cgcgtccggt cccggcgagt tgagcgggac gtagacctgc
    26401 atgcccttgg atcccgaggt cttggccacg cagtcgaggt ccgccgcggc caggagatca
    26461 cggatctcga gcgccaacgc cgcgcactcg gggatccccg tccccggtcc ggggtcgagg
    26521 tcgaacacgc acatcagcgg gtgggcgagg tcgtcggagc gcgccatcgg agcgtggagc
    26581 tccagggcgg ccaggttggc ggcccacacg atggcggcgg gttcgtcgaa gcggcagtag
    26641 cgcagctccc ccttgcggtc accgggtccc accgcggtcg gcatccactc ggggcggtgc
    26701 gacgggcacc gcttctcgaa gaacgacggg ccctcgaccc cgtcggggta gcggcgcatg
    26761 gtcatgcacc ggccccgggt gtgggcgacc atgaccggcg cgatccgggc gtagtagtcg
    26821 aggacctcgc ccttggtcgt cgacgtggcc gggtagagga ccttgtcgag gttggtgagg
    26881 gtgagggttc gcccgtcgag gctgaccggc gtgacgtcgg ccatgggttc gtcgacgggc
    26941 gtggcggcgg ccgggacgga cggcgctcag gccgagcgac gggtggcggc cggcttgctg
    27001 ggggcgggct cctccgcggg gctctcctcg ggggcagcat cctcgacggg gaccgggttg
    27061 tcatcagccg tgctggggtg gcgaccgcgg gcttcgcggg ccgcggcgac cgacgcctcg
    27121 agcgcggcca tcaggtcgat gacctcgtcc tcggcgggac cctcggcaac ggccaggaca
    27181 cgctcctcgc ccgcgtcctt ggccgcgatg aggtcgagga gctcgtcgcg gtagctgtct
    27241 cggaatgacc cgggaacgaa ctcggccgac agcgactcga tcagctgctc ggccatggtc
    27301 agctcccgct cggacacctc gaccgagtcg agctggtcga actcgtcgat gtcggtggcg
    27361 gggttgatct cgtcctcgta caccatcatc gacagcacca gtcggccgtc ccggggacgc
    27421 atgaccgcca cgtactgctt ggcccgcatc acgaagcggg cgacggcgac cttgccggcc
    27481 ttctccatgg cctcgaccag cagtgcgtac ggcttggccg cccggtcatc gggtgccacc
    27541 cagtaggcgc cgtcgaagta catggggtcg atcgaggaca ggtcgacgaa ctcctcgagg
    27601 tcgatcgtgt gggtggcccg gggctgcaac gacgcgagct cgtcctcggt cacgatcacg
    27661 tagcggccct tcgagacctc gtagcccctg gcgatgacgt ccgcgggcac gtcggagccg
    27721 tcctcggcgt tcaccttccg cgtccgcacg cgggacatgt tgcgggtgtc gatctggttg
    27781 aaccggaccg tccgctcggt gacggccgag aacagcttga ccgggatgtt gatgagaccg
    27841 aagctgatcg atccgctcca gatggcacgg ggcacgggcg cgctccttca gacgaccgct
    27901 ccgtccgcgg ccggtccagt atcggccccc agcgggtcga tttccaccac cggcgtccga
    27961 acggacatgc cgttcaggac gacgaccagg cgcggggtga ccactccagc tccgcccaca
    28021 ccgccttggt gcccccgtcg aggatcaccc cccactgagc ggacagggcg gcgacgatca
    28081 tcatcccccg gcccgccggt cgttccggcg ggggttgtcc cggggcgggc aggcgcgtcg
    28141 ggtgggggtc cgagacgacg accagcaccc cgtggtcgag gagctccagg tcgaccgtca
    28201 tccagccggg ggcgtgctct tcgacgttgc tcaccagctc gtcgaccacc agcaccacgt
    28261 ccgcggcggt cgaccggggc accgctgcgc gacgcagggt gtcggcggtc caccgtcgga
    28321 ggcgggccaa cgaggtgggt gtggtgtcgc cggtgaaccc gtcggcccgg ccggcgaggc
    28381 acatgacgac ctcgcccagc cgggacctcc acctgtcggt ggtcgccgcg gacccgccgg
    28441 cacccgggtg gaccagggcg atcgtccggt cgggatcctc ggcccggagt ccgtcgaccc
    28501 gggccgacag gagcgaggcg tctccgtcgt cgggtccgac gtcgagctcg acgatcggct
    28561 gccgggcagg atcggcaccg ccggaccacc aggcgatgtg cgccggaccc ggcatcgacg
    28621 cgccgacgga ccgcgccggg ggcgcggccg atggcacgga aggggcgacg gctgggtcgg
    28681 tcatggtctc tggactccgc cgggatccgt acccctgccc cgacaccgtg aaacccgacg
    28741 ctcaggtccc ggccagcagg gcccgggcga cgaggtcggt gccgaacgcg gtcaggatcg
    28801 ccaggtacag caggccggtg gcggccatca ccgccgagta gtagcgctcc tgcagcgcct
    28861 tgcgggtcac gacgaccagc acgatcgtcg tgacggcgca cgccagccag aaccacccca
    28921 gcatcccgcc gtagccgtcg tcgatcgttc cgttcagcac ggccaccatg ccgaccggga
    28981 cgagctggag cgccacctcg gggacccagg tccagccgac ccagcggacc agctcgttga
    29041 gcgggtcgcg cgggagcccc ggttgggtga ggtaccagtg tccgagcagc atcgcgtcgg
    29101 tgacgctgcc gaggaacgcg gcgccggcga cggtgcgcgc cacccccaac cacggggggc
    29161 cgcctacgtc gagcccgccg gcgacgacgc cgacgaggcc gatcgtcggg gcgatcaggt
    29221 cgaggtccgg cgggaactcc ggggcgtcgg gatcgaaggt ctgctcggca cggtcgatgc
    29281 cggtcatctg cgccacgcgc tccgatcggg cctgggagcg ctcccgctgg agcgcgaccc
    29341 cggccgatcg ccgcaggacc gacaccacca gcacggccgc ggtgaccacc acgacagcgg
    29401 ccgaggccag gtcccgccac agcagggggt cgtagcggag tccggcgacc agcgcgccca
    29461 gggccatgag cccgtaggtg cttcgcagca gccagccgta gccgagcgac acctcacgtc
    29521 gtcgggtggt gacccacagg aacgcgagcc caccggtcgc ccactgcagc aggaaggtgg
    29581 cgacgttgag cgacatcggc cgggcaacct accgcgccgg gccgcggacg ccctcagcga
    29641 gagccggggg ccggtcgcgg actccgaggg ccgatctcgg cgacgtcgcc gtcagagctg
    29701 ggccagggcc cggtagacgg cgtcgaccag gttcacccgc cggaacgaca gggcggtgtc
    29761 ggcgtcgagg ccagcgttga acaggaccga cagcaccacg ccgttgtcgg ggtcggccat
    29821 ggcgaagctc gtcccaccct ggccggcgtg gccgaaggcc cgcggggacg gcccgtcacc
    29881 gaagaagtgc gacgacagcg gtgtcatgaa ccccagaccg aacccggcgt cgcggtcgag
    29941 ggtgacgtcg tggttgagcg gcaggcgggc ggtgacggca tctgccagca ggtccggggg
    30001 cagcacgacc tcggcaccgt cgaggtcgcc gagcaggccc tggtagaagg tcgccaccgc
    30061 cgacatcgtg ccgtaggtgc cgaaggcggg gttccactcg cacgcggtct cgggtccgac
    30121 ctcggcgagc aacgggaccg gttggtccat cgtcaggtcg taggtggccg agatgcggtc
    30181 gtggaggcgg tcgaaggtgg ccgggtcgaa gcggaccacc aggtcgtcgg ggtcgacgcc
    30241 gtagggcacc agcacgcggt cgcgcacgta ggacgagtac gaggcggagg tgagagcctc
    30301 gacggcacgg ccgagcaggt accagccgcc gaactcggcg taggcgcggt cgaccccgta
    30361 gcgccacgcc atcggcggtt ccgacgcgac cagccagccc tcgcggctgc gttcggggag
    30421 gatccgggcg aacaccgtgt tcatgacgtg caggccggcg gtgtgtccga ggacctgctc
    30481 gacggtgcgc tcggcgatcc atggcgcgcc cagctcgtcg aggccgtcga tgacgtcacc
    30541 gagacggtcg tccaggctca gctcgccctc ggcgaccagc cggagcaccg ccaccgccac
    30601 gagcggcttg gcggtgcagt acagcgccga gagggtgtcg cgggtgaacg gctgctccag
    30661 gtgcgtcgtg ccgaccgccg tgtcgagcac gcactccccg tcggcgacga cgacgatctg
    30721 cgccgcggtg tggaagaacc cctcgtcggc ctcctcggtg aggcgttcga cgagctcctc
    30781 gaacggctcg gtcacgatcc cctcggtcac ggtctgctcg gtcacggtcc cctcgctggc
    30841 gccgctgtcg gtcacgccgt cgacggtacc gccccctggg tcggcaccgt cgacggcaac
    30901 cggcgtctcc gccggtcggc tcaggcgccg gcctgttccg ccagcagggc ccgggcgacc
    30961 gcggagacct ggatctcgtc ggtgccggcg tagatctgca gcaccttggc gtcacgagcg
    31021 agctgctcga cctcgaactc ggccatgtag ccgttgccgc cgaagatctg caccgcctcg
    31081 agcgccacct ccatggcggc ctgggcggcg tagagcttgc ccgccgatgc ctcggcgaag
    31141 ctgcccggag cgccggcggc ggccatctcg atgttgcgga acacctggtt ctggaggttc
    31201 atccgcgcca cctccatctt ggccagcttc agctggatga gctggaactg gccgatgggc
    31261 tgaccgaact ggacgcggtc gttggcgtag gcgaccgaca gctccaggca ccgctcgacg
    31321 atgccgaggg ccatggcgac cacaccggtg cgctcctggc cgaaggtggc cttggacgcc
    31381 tggcgcccac cggctccctt gtaggcctcc tcggactcgc cgaggagccg gtcggacccg
    31441 acgcgcacgt cgtcgaggaa gagctcgccg gtcggactcg agtgcatgcc catcttgcga
    31501 agcggcttgg actgctccag gcccgccatg ccggcgtcga gcacgaactg cacgacggtg
    31561 cgctcctcgg gcgggttgcc ctcgtcgagc ttgcagatga acacgatcgt gtcggcgtag
    31621 ggaccgttgg tgatgaaggt cttggacccg ttgagcacgt actcgtcgcc gtcgcggcgg
    31681 gcggtcgcct tcatcgaccc gaaggcgtcc gagcccgagc cgggctcggt gatcgcccag
    31741 gcgccgatct tctccagcgt cagcaggtca cgtccccagc gctccttctg ggcgatggtg
    31801 cccttggaca tcaccgacgc cgcggtgagg cccatcgaca cgcccatggc ggtgaccatc
    31861 ccgggacaga cccggcacag ctcgatgatc gggatgatct ggaaggcgat ggcgttggcg
    31921 tcacgggtgg cgcgctcctt ggcgtccccg ccggtgcccg cgtcctgcga cgccgtcccg
    31981 tcggccgcgg cgcgctcggc cgcgatgcgc gagtcgaagt tggccttctg catctcggtc
    32041 atgccgaagg tggcgaacat cttgcgcagg aggtcgtagg gaggcaggtc gccgaactcc
    32101 agggcgtcgc ggtgggggcg gatctccttg tcgacgaagt cgcgcaccgc ggcgcgcacc
    32161 atcagctgct cttcggacca ctcgtacacg gctcgctcct gttcggggcc ggggttcttg
    32221 accgttcggt caagtgtagg gcagtgccga tcagtcctcg gccggcggga tgtgcggggc
    32281 gacctcgccc caccagccca gctcgtcggg cagcaccggc aggccccggt acgccgccac
    32341 gtactcgggc gactggatca tcccgtgcag gagcagcagg agggtccaac gggcgtcgac
    32401 cgatcccccg tcctcgatcg cctcgagcgc cgtcgcctgc ccggagcgca tcttcaggta
    32461 gttgcggaac gacatcgaca cgtgcatctc gggggccccg aacgcgggct cgtcctcggt
    32521 cgggtccgtc cagtcgctca ccagccaggc ggttcgggac ccgtcgtggt agcggacgtc
    32581 acatcgctgg gtcccgaccg gggtggcgaa cacctcgaag cgaaggcggg cggtggcgtg
    32641 cgggacgcgg gcgagggact cgaaggcggg ggccggcagg tcgtcgagca ggggcagggg
    32701 ccggatctcg tcgcccagcc ggagggcggc cccggtcacg tcgtcggggt cggccacgcc
    32761 tcccggacac ggacgcaacg gcgagccggg acggatcagc gcccacgggc ccggttcgtc
    32821 gtcctcgatc cagtcgacca gcacgccgct ctcgaagcgc tggtggtggt ggacgaccgt
    32881 gccgtcctcc aggatcgcct ggtggtgcac cgtcccggtg gcgtactccg gcaggtgcag
    32941 gtcgagttgg cccgacccgt cctcccgtgt cgactcctgg tcgaccgacc ggcggaactg
    33001 cgcgacgaat cccatcggtc aagtctggcc cagcagcgac cgggtcgggc tcagccccgc
    33061 ctcaggacct caccaccacc tcggcggcgg gaccggtgac gggatcggcg gagtccgcgg
    33121 cggcgcgggc gtggtagctc gaacgtgtga acgggctcga ctcgacgtgc gcgatgccca
    33181 ggctgcgacc gacggcggcg aagccggcga agtcgtccgg ggtccaccac cgggccaccg
    33241 gcaggtggtg cgaggtgggc cgcaggtact ggccgatggt cacgatggac acgccgaccg
    33301 ctgcgaggtc cgccagcgtc gaggtgatct cgtcgcgggt ctcgcccagg ccgacgacca
    33361 ggcccgactt ggtggtgtgg ccggccagcg cggtccggcc gagcagcgcc agggaccggg
    33421 cgtagccggc cgacgcccgc acggcccgtt gcaggcgggc gacggtctcc acgttgtggt
    33481 tgaccacgtc gggttccgcc gccagcacca cctcgagggc ggcggcgtcg cccttgaggt
    33541 ccgagatcag tacctcgacg cgcgttccgg ggcgtcggcg ccgcaccgcc gccacggtgg
    33601 cggcgacgtg cgccgcccca ccgtcggcca ggtcgtcgcg ggcgaccatc gtgatcacgg
    33661 cgtacgacag gtccatccgc gccaccgcgt cggcgacccg ttcgggttcg tccgggctcg
    33721 gcggttccgg gtgccgcgtg tcgaccaggc agaagccgca ggcccgggtg cagcgctcac
    33781 cgcacaccat gaaggtggcg gtgccgtcgg accagcactc gctgaggttc gggcaccccg
    33841 cctcctcgca caccgtgacg agcccgaggt cgcgcaccgt ccgcttgacc ccgagcacgt
    33901 cggcgtcgtg gcgcacccgg gcgcgcatcc agtccggctt gcggtcggtc aactcgaccc
    33961 ggtcggtcac gccggcctcg gccagccggc cgagcaggcg caccgaggtg cccccggccg
    34021 tgctgacacc actgctcccg cccgtacctg tgccggcgcc gccgaccatg gcgttcgcgc
    34081 ccgtggtcgg ccggcccggg ccggcacccc gactgaacgg ggccaggtcc gagtcgtcgg
    34141 gtgccgcgcc ggcgtgggag cgccgccaca ccacgtcgga gcggtcgaca tcgagtccgt
    34201 cgctctggcc gccacgggcc gcccagcccg cggcgaaggc agcggcgaac gcgtcgacca
    34261 ccgcggccat ggatgcgtcg atcccctcgc gggccaggga ggtgacgccg tagccggtga
    34321 tcccgcaggg cacgatgtgc tcgaagaacg tcatgtcggg atcgacgttg agggcgacgc
    34381 cgtgcatggt tcggccacgc tccacccgca ccccgacctg ggccaccttg cgggcatccg
    34441 cggtgccggg ccggatccag acgcccgggt agccgtcgtg gcgaccgacg tcgtcggggc
    34501 cgagacggcc ggctgtgtcg acctgccgga tcgtgtcgat caggaccgat tcgagcaggt
    34561 gcacgtagtc ccgcaccccc agcgccgggg tcgatcgcgc ggccggctcg cccgccgcgg
    34621 cgggatcgac ggtgacgatc gggtacgcga cgagctggcc cggaccgtgg aacgtgacgt
    34681 caccgccacg gtcgacctcg acgagttcgg caccgaccga gccggggtcc accaggacgt
    34741 ggcggccgtc cgcgttgcgt ccgagcgtgt aggtgggtgg gtgctcgagc agcaggaggt
    34801 gctcgccgat ctccgggccc gaccgaccgt cgtgaagggc gcgctgcagc gcccacgcct
    34861 cgcggtaggg gacggtgccg agccagcgga tccgcagcgc cgtcacgtca acccagctcg
    34921 acggcccagt cccgggtctc gatgatctcc ttgagccgcg acatgaacgc cgcggcgtag
    34981 gcgccgtcga aggcccggtg gtcccagctc agcgtcaggt tgcccaccga gtggataccg
    35041 atcgactcgt tgccgtgctc gtcgagcacc acgaccggac ggcgcgacac cccgtcggtc
    35101 gacaggatcg ccacctgggg ctggttgatg atcgggatca ccatggcggt gccgtagctg
    35161 cccgagttgg agatggtgaa ggtccccccg gtgagctcgt cgggaccgag ctgtcgggac
    35221 cgggcccggg cggccaggtc ggtgatctcg cgggccagcg ctcgcaggcg tcggtcgtgc
    35281 gcgtcggcga tgaccggggc gagcaggccc tggtagtcga ggtcgaccgc gatggccagg
    35341 tgcacctcgt tgtgcacgac gaggtgtccg tcagcgaccg aggcgttcat gtgggggaac
    35401 tcgtggaggg cgtcgaccac ggcccgcgac acgaacggca ggtaggtgag ggcgaagccc
    35461 tcctggtcct tgaacgcgga ccgctcgacg cgccggacgc gttcgaccgc ctcgtagtcg
    35521 acctcgacca tggtgatcga gtgcggggat gtggccatcg acatgaccat gtggtcgccg
    35581 gtcatgcggc ggatcttgtt caacggctcg accgtgtcgc cgctgcccgg tcgcatcggc
    35641 ccgctggacg gtggcgccgg acgggaccgc ggcgccgccg gggcgaccgc agcagggctg
    35701 ggagcagggc tgggagcggc gaccacgggt
//
LOCUS       DAOQOD010000010        34038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_410_length_34038_cov_10.840420, whole genome shotgun sequence.
ACCESSION   DAOQOD010000010 DAOQOD010000000
VERSION     DAOQOD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 34038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34038
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_410_length_34038_cov_10.840420"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..790)
                     /locus_tag="PLZ72_02295"
     CDS             complement(152..790)
                     /locus_tag="PLZ72_02295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNA35157.1"
                     /translation="MPLLLCDLDETILERREALERWAAGFARDRGLPSGAVRAILDED
                     HHGARTRPQFVEAVNHRLGLEPPLTLDYLHDYVACFTLEPDNAAALRRARLAGWTIAI
                     ASNGEQPQLDKIAVVGLSAYVDAVAVSAIDGVRKPDPGLLRIAAERAGASLEGSWMIG
                     DDPLNDVQAARSAGIRSVWLRRGRTWPDGLEPPTEQADSFAAAVDLVLDSAP"
     gene            895..2277
                     /locus_tag="PLZ72_02300"
     CDS             895..2277
                     /locus_tag="PLZ72_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35158.1"
                     /translation="MSRRLEIELTSERPDGTWTWRAAGAKLPKGDLSGSLLPGGSKVG
                     DVLRAEAEFLIDGIDIVEVLAPKGTRKQPELLEIMGSGREEPLVTTQLVSRGRGEGRG
                     RRRDRDGDGPRRERRDRGDRPDRPRGPRPEVPERPKPKRLRPGRAHRNAVIAAVPEEQ
                     RAIAEQVLRGGVPAVREAVVAQNEQARTENRPEQPVDQVVAMAESLYPRLRDAEWKDR
                     AAAAIADLDELDLRDLRSVVVAGDGASHDDEARAMLEELRTGLTRRVDEEHANWVADI
                     AANLDAGRFVRALRLSSRPPKAGTPVPTELSARLVTAVSEGLTPGTNQELWAAALDAL
                     AFSPVRQQVTPAGRPETPTDDLLEAVRRVADRVPAIAALFGVDPSEAAAAKRRRPPRG
                     RSGAGRGAKPGDKSSTGSGKTADDKTADDAASDDAASAGTADAEAVVEVAEAETAPTE
                     PVQAESDQAK"
     gene            complement(2317..2868)
                     /locus_tag="PLZ72_02305"
     CDS             complement(2317..2868)
                     /locus_tag="PLZ72_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNA35159.1"
                     /translation="MTAIDALVFDFDGTLVDSDDALIRPLLALGVPTEEITFGHAVAE
                     ECERLGIPMESYVGLYDDSAAQPFPGVDDVIGQLGRWALCSNKHPRPARSELARLGWN
                     PEVALFADSFDWAHKELGPVLDALALTPDQVVMVGDSAGDVRVAEAVGCRYVWAGWNP
                     RVRAARPHGRVLDRPEQLLDLYG"
     gene            2986..3762
                     /locus_tag="PLZ72_02310"
     CDS             2986..3762
                     /locus_tag="PLZ72_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA35160.1"
                     /translation="MTIEYTKQGNVGIITINRPDARNAVNSDVAQGIEDAIDQIEADD
                     EVWVGVLTGAKTDKGYIFCAGADLKQMAVDPGGMQTARGGFAGFVTRERNTPIIAAVD
                     GPALAGGTEIVLAADMVVASRTAVFGIPEVKRNLVAAAGGLFRLPRKIPRNVAMELAL
                     TGRLDFPAERAYDFGMVNRLCDEGQALETALGLAGEITENAPLAVAESRRIMIEATDQ
                     PDEVGWKLSGEGIIKMFGTEDFSEGLTAFAEKRKPNWKGR"
     assembly_gap    3835..3934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3935..4183)
                     /locus_tag="PLZ72_02315"
     CDS             complement(<3935..4183)
                     /locus_tag="PLZ72_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35161.1"
                     /translation="MAEEPPPVLRAAAVVAHGNDLLMVRSADGGGERGWSVPSAPVRG
                     GETMVEAVVRAVRSLTGLDALCGAFLGWYEVLPAESDRG"
     gene            complement(4176..4601)
                     /locus_tag="PLZ72_02320"
     CDS             complement(4176..4601)
                     /locus_tag="PLZ72_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA35162.1"
                     /translation="MGHHRGGDVYQEREGRPDPLRDSLDADLAAADAGSALVLVGGLG
                     PVAVGYAVVRLEETVNGRLAVVSDLFVEPDARGVGVGRALMEAVVEWATDSGCHGVDA
                     EVLPGDRSTKNFFEAFGLVARKITVHRSLGERSPDGSRG"
     gene            complement(4714..5184)
                     /locus_tag="PLZ72_02325"
     CDS             complement(4714..5184)
                     /locus_tag="PLZ72_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35163.1"
                     /translation="MNPLQIPTLLAREVSEAVPALVERTRAQVELTQRLVGQLPCLSN
                     LWGQPGSAAGDRSARTPEDSDRDDREGADGADHPEPAPTAVEADVRDQLPTVDVQDLA
                     IPDYDSLAASQVVPRLASLDPAELALVLTYEQSHRSRQTILNRVRQLQAATNGS"
     gene            complement(5194..5709)
                     /locus_tag="PLZ72_02330"
     CDS             complement(5194..5709)
                     /locus_tag="PLZ72_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35164.1"
                     /translation="MDDDELLEEELDEEEGLEDEDALDDEIDDEIDDVDLDGEEDADQ
                     VEGEDDEDDVPAAKAKRPSSADDDDEDDEDVDPDDVEADLDTILKDRIAAGADEDDED
                     ELEEPGTESTERVAAKTADEFTCPTCFLIVHPRQFGRSGALSCPEGYDPCGAIEILTR
                     GTKAPARAKKG"
     gene            complement(5939..7561)
                     /locus_tag="PLZ72_02335"
     CDS             complement(5939..7561)
                     /locus_tag="PLZ72_02335"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNA35165.1"
                     /translation="MTALPTYVAEQFTPEEAAVLRRYFTSLDGPVFALVNLPEVVKGA
                     LFARYSRSAKSLRRLFLDEFVGELDTTGDETVDATIGLERAESLYDRVFFEYGDDSVA
                     QLGGVHLACEQASNLLTKVLEWGRLMSYLEQSTRYIPYDERLGGRYRYHRDPEILGSP
                     LGARYVGDMDQLFDSYSALATDMSEHFRDRHPKSETDSDFVYRQAIRAKAFDSVRGIL
                     PAASLSNVGIYGSGQAFEALLLRMRSHPLPEARQYADLMLVELRKVVPSFLKRVDLPD
                     RGVTSSEYLRSNREAMEAVAYEMLSGDGVDGPTDPGVELVDFDPEGELKVVAAMLYPY
                     TGLAESTLIERVRTMSTEDRLAVMRAYVGDRTNRRHKPGRALERTDYRFDVVSDYGAF
                     RDLQRHRLMTIEWQDLTPAHGFTRPAAIDEAGMTARFDEAMERSSGLWAVLAERFPER
                     ASYAVSLAYRIRYVMQFNAREAMHLIELRTSVQGHPSYRAIGQRMHHLIAERAGHRAV
                     AEMMRFVDHSGEAELERLGAERRAEARRSESA"
     gene            complement(7609..8820)
                     /locus_tag="PLZ72_02340"
     CDS             complement(7609..8820)
                     /locus_tag="PLZ72_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNA35166.1"
                     /translation="MNTARPDHTAQDPAADTTPTASPDAVDAAFAAVDRRADRLLSIS
                     HAIFDRPELAFDEHHAHDLLCDALEAEGLSVTRHAHGLDTAFVAEAGSEGPTVAVICE
                     YDALPGVGHACGHNVIAAAGLGAGLAAAELADALGGRVRVIGTPAEEGGGGKVFMLRD
                     GAFDDIDAAVMVHPADHDLPTMSAIAIQRLDVTYTGRASHAAAAPWNGVNALDAAVLG
                     YVNVAALRQHIRPDERIHGIFTEAGEQANVVPRRAAASWYVRSPSVEGLAELKERVLA
                     CLAAGASAAGCRMDHEWLDPAYDDLVDDRWLLDSWVRHSTAVGRPPQDPDTVGPVVGS
                     TDMGNVSHVVPSIHPMIAVAPPGVAIHTAEFATHARGAGGDAAVLDGAKALAGVVLDR
                     WAAGSPAGSRR"
     gene            complement(8844..9833)
                     /locus_tag="PLZ72_02345"
     CDS             complement(8844..9833)
                     /locus_tag="PLZ72_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNA35167.1"
                     /translation="MRAWYLDESPGRYQWGEVPDPEVGPADVRVRVRATALNHMDLWL
                     TTGLPKPPRFPHVPGNDVAGVVDAVGERVTDWVPGDEVVINTAVVPADALARGDDSVL
                     DPAMMLLGESCWGGHGEFCVVPGHQLERRPAGRSWAESASYPVCYTTAWRLLRRARLQ
                     PEDTVLVTGIGGGVATAAMMLARHVGARVFVTSRDAAKRDRAIELGAEGAFPSDEPYP
                     ITADIVVDSIGPATWDFAFRTLRHGGRMCVCGGTSGPKVELNLPRLFFKQIDVIGASC
                     GSQSEFRHVTDLLGDGLPVVVDEILPLSEYPRALERLRSAEQLGKIVLEHPEP"
     gene            complement(9865..10500)
                     /locus_tag="PLZ72_02350"
     CDS             complement(9865..10500)
                     /locus_tag="PLZ72_02350"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35168.1"
                     /translation="MTIRRGEPWGAAGPLAADAPVAAGDGELADLVAAGLAAGRPVTE
                     VGLLGGDLHAALGAPGHTEADLRSGAGMRLPIDLGEVVVDRGDGLISRHVFVAHATAG
                     RGRRRWSSRTVVAMNGTHLAGADLGPRAHPNDGLLDISDGRVPASQVRAAGRRERTGS
                     HVPHPGILERRTAAYEVLDERELPIRVDGRLIGAATHFELRCLPDALVIVV"
     gene            complement(10497..>13253)
                     /locus_tag="PLZ72_02355"
     CDS             complement(10497..>13253)
                     /locus_tag="PLZ72_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA35169.1"
                     /translation="DRARHDRRRRVLTDPFAHDFPLDRFQREAIEGIDAGRNVLVSAP
                     TGSGKTVVGEHAVARALAVGAKAFYTAPIKALSNQKYADLVAVLGPGRVGLLTGDHTV
                     NADAPVVVMTTEVLRNMVYAGSPALDGLQWVVLDEVHFLQDAYRGPVWEEVLVHTPAA
                     VRFVCLSATVSNAAELGEWIEALRGPTDTVIEHVRPIRLDNLYMVGDRSAERDHLLPV
                     LVDGRPNPEADRFDAERSGAGRHRGGSGRPRGGRPRGGRRFRTPRRLEVAERLDDEGL
                     LPAIYFIFSRAACTDAARHCRDAGLRLTDAAERSRIRALVEARVATLSDSDLDALDYD
                     QWLDTLEAGVAAHHAGMVPAFREAVEDCFNEGLIKVVFATETLALGINMPARSVVIEK
                     LTKYNGDTHEFLTPAQFTQLTGRAGRRGIDDQGTAVVLWSPFVTFTQIARLVASREFP
                     LTSAFRPTYNMAANLVHRYDRDDAHAVLARSFAQFQADRAAVTLQTRLDRIRSELDLL
                     GGPPDGDADRAVADYVAALDTLAATTPRPQDRRERIGASLAALSPGDVVSVPHRDPGR
                     PEQPLLVLSVAFRKGGAVRVRTVAANGADKELTAGDLASPVWPIARVDLPVPFEPGRS
                     AYRRDAAAALRRTNLRGARGRAAKGRRRHDDHLDDVEPAVLEAHLAARAALESHPLHH
                     DDDRDAVVARHRERARLAHDLEVARRRLARRGSGLVNRFDAVLEVLARSGRTSGWSLT
                     DSGERLRRIYHECDLLISLALDDGLLADLDAPAMAAMVSCFTYEHRSPDAPPPPTHPS
                     PELRRRFKRLEQIHSRLNGWERAARVPETRAPDAGFAATAHRWAAGRPLAAVLDDDLT
                     GGDFVRNTKQLIDLMRQLGEVAADPRTAAVCRRAADSLFRGVVEAGSTVSSGGSDVTP
                     GGGS"
     assembly_gap    13254..13353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13354..14174)
                     /gene="tatC"
                     /locus_tag="PLZ72_02360"
     CDS             complement(<13354..14174)
                     /gene="tatC"
                     /locus_tag="PLZ72_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNA35170.1"
                     /translation="MAVLKGAEREGASMALGDHLRELRRRLLICVGTVAAMLIPAWFL
                     YPWMIDVLNAPYCDALTNLDPDASCKFLETNLLDPFTLRLRIAGYGALFLAMPVILWQ
                     LWRFIAPGLYKKERRYALAFTVSAMVLFVAGAVTAYLTLTQAVNFLVSIGGPDVEIRS
                     GIGNFVKLALFMMLAFGVGFQFPVVVVALQMIGVVTPERLSSWRRQAIVLITIIAAGI
                     TPSGDPISMFALAIPMYLFYEISIVIGRLWNRRKRKAASREAEAEKERRAARAARR"
     gene            complement(14177..14593)
                     /locus_tag="PLZ72_02365"
     CDS             complement(14177..14593)
                     /locus_tag="PLZ72_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNA35171.1"
                     /translation="MIQNFGGGELLVILILALVVLGPERIPEMAKSAGKMIAKLKTMS
                     SGLQGQVQGVMDDPAMQPLRELGDLAARPRQKLAEYALEAEADERARSERASIDAATE
                     SGAAAEPTGTDPAEDASTGDDSGDGSSDDTGERETA"
     gene            complement(14690..15019)
                     /locus_tag="PLZ72_02370"
     CDS             complement(14690..15019)
                     /locus_tag="PLZ72_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35172.1"
                     /translation="MALIRATCSDCGDVELRSRDLRVRQCRDDESATYLFRCPVCRMI
                     EVRPAEQHVVDVLLAAGVHSTEWHLPAELTERRGGAPITHDDVLDFHDLLSTPDWFTT
                     LTAINDR"
     gene            15176..15904
                     /locus_tag="PLZ72_02375"
     CDS             15176..15904
                     /locus_tag="PLZ72_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNA35173.1"
                     /translation="MAERLPSLVDPPILFAHRGARAHAPENTIEAFTLALRLGATGVE
                     SDVWATADGAAVLDHDGLVRRGLRRSAIGGLPRADLPEGIPTLDEMYEAIGSDVQVSL
                     DVKDPDVLDEVLAVARRAGASENLWLCHPDLDLLADWKQRCGAVRLVHSTRLGALGGK
                     PEPHAARLRELGIDAVNLHHSEWSGGMVALYHRFRRVAFGWDAQHERVIRSLLGTGID
                     GVFSDHVDRLVDVAGEGSEPGPTW"
     gene            complement(15924..16520)
                     /gene="lepB"
                     /locus_tag="PLZ72_02380"
     CDS             complement(15924..16520)
                     /gene="lepB"
                     /locus_tag="PLZ72_02380"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNA35174.1"
                     /translation="MSEPTVDQPAASGRPAARTRSRSSTRNMVEWAVVIVGAILVAFL
                     VKTFLVQAFEIPSPSMEMTLVKGDRVLVNKLSYDLHDVNRGDVIVFTRPASLSGGPDD
                     PKDLIKRVIGRPGDTVQSRDGKVYVNGRLLEEPYLQKGVVTDGMDEPVTVPEGHVWVM
                     GDNRGDSADSRVFGPVPEDSIVGRAFMRMWPPARIGSL"
     gene            complement(16541..17485)
                     /locus_tag="PLZ72_02385"
     CDS             complement(16541..17485)
                     /locus_tag="PLZ72_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA35175.1"
                     /translation="MSPATASERVERILSVLPWIVEEPGTTVQQVCDRFGMTERDLRA
                     DLDLLMYEVGIHPFTPDARVDAYVEDDGRIQIHLGDYFRRPLRLTPDEALVLYAAGQA
                     LLDRPDPDPVLARAVAKLAAVLGDGADDSVDVRLGDADPDVLDAVRRATDAGQRLRID
                     YYSFGRDERTEREVDPLRLSARDGHWYLRAWCHRAGGVREFRVDRIDTAVPTGGAVDD
                     HPNVEQADVLDLSDGRPVALVVGAEDEWIVDAVPTESVTTDADGRIRVVLRVTAEPWL
                     QRLLLRLGPTVEARDVDTGEDLRPLGATAARRVLRRYR"
     gene            complement(17482..18414)
                     /locus_tag="PLZ72_02390"
     CDS             complement(17482..18414)
                     /locus_tag="PLZ72_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA35176.1"
                     /translation="MAKVDRLLNIVAALQASSVPLTAEEIRERVPGYESHSDDTFRRT
                     FERDKDELRQAGVPVETVMVAHTEQPRAAYRIRRDRYELADPGLSPEELAALHLAATS
                     VRLVGIPVDEVEDALRKLGGTGGGGAAAPVGSLDAPDALAVVFAAVLDHAVITFGYSG
                     DERHLEPHRLQYEQGQWYVSGHDLDRDATRSFRLDRVAGPVTAGDAGAFTPPDDLPGV
                     RLRPWELGPGPAETAHVLLEPTVAASVLAEDPELTVSATQPDGSVVIELEVRNPAGLR
                     SFVLSHLDHAELLDPPGHRRDLIDWLGGFERLSS"
     gene            complement(18421..19503)
                     /locus_tag="PLZ72_02395"
     CDS             complement(18421..19503)
                     /locus_tag="PLZ72_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HNA35177.1"
                     /translation="MPTFRTATVTEVLSARPGLQRVAVAMADGSPARAYALVELVGEC
                     AVGDEVVLNTTAVELGLGTGGWHVVHWNLARRELVAPGPDHVMKMRYTSLQADVGTSE
                     LVHPECDRPIAGVPVVACTVHSQVPLVALAVAERRPGTRVAYVMTDGAALPLALSDLV
                     AQLCADGTLVGTVTAGHAFGGDLEAVTVASALGLAVHTLGAEVVVVGMGPGVVGTGTA
                     LGTTAIEAASVIDTAAALGGDTVLCVRASAGDGRERHRGVSHHTETVARLSRTSPRVA
                     SSPSELVEELSELTGVQPLVVSDTPDPADLLGSAGLRVTTMGRDVSEDPIFFAAAVAA
                     GALAADLVADRATAPVTPRSADRPVH"
     gene            complement(19516..20874)
                     /gene="pafA"
                     /locus_tag="PLZ72_02400"
     CDS             complement(19516..20874)
                     /gene="pafA"
                     /locus_tag="PLZ72_02400"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HNA35178.1"
                     /translation="MDRRIYGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLANGARLYLDVGSHPEYATPECDSLADLVAHDKAGERILEQLLESAEQRLAEEGI
                     RGDIYLFRNNTDSAGNSYGCHENYLTSRHDDLTHYTEVLIPFLVSRQIYAGAGKVLQT
                     ARGAQFSMAQRAEHIWEGVSSATTRSRPIINTRDEPHADSDRYRRLHVIVGDSNMSEY
                     ATYLKVGATALLLRMLEDPTVVLRDMTLENPIRAIREISHDITCTRRVRLANGREASA
                     LEIQTEYLNRALRYADNHDLSPEEKQSLDMWEHVVTGLERDPLSLDREVDWVTKHNLI
                     EAFRERHDLPLSHPRVALLDLQYHDISRNRSVFYKLQRAGRVERIVTDADIEEAVDTP
                     PQTTRARLRGEFIRTAKERKRDYTVDWVHLKLNDQAQRTVLCKDPFKAHDERVERLIE
                     SL"
     gene            complement(20937..21620)
                     /gene="prcA"
                     /locus_tag="PLZ72_02405"
     CDS             complement(20937..21620)
                     /gene="prcA"
                     /locus_tag="PLZ72_02405"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNA35179.1"
                     /translation="MTMPFYVAPEQMMKDRADYARKGIARGRALVACTFDSGILLCAE
                     NPSRMLRKVSEIYDRIGFAGVGKYNEFDQLRIAGIRHADLKGFSFSREDVDARSLANQ
                     YAQILGQVFTHEMKPMEVEILVAEVGHDPAGDQLFHILYDGTLIDETGWAVLGGEAEA
                     IGERMGSAWVEGGTLAQAVNAAVTALAGPERTIPVTELEVAVLARGNGRRTFQRIEDD
                     ELTPLIEPS"
     gene            complement(21617..22480)
                     /gene="prcB"
                     /locus_tag="PLZ72_02410"
     CDS             complement(21617..22480)
                     /gene="prcB"
                     /locus_tag="PLZ72_02410"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNA35180.1"
                     /translation="MNLPLYSPQDDPGPNFAELVRRAGLVPPLPDPTGTAAATGATLG
                     GAPGIGHGPGLTVTHGTTCVAVRYADGVVMAGDRRATSGNFISHRSIEKVFPADRHSG
                     VAIAGAAGPAVEMVRLFQLQLEHYEKVEGSPISLEGKANQLSQMVRNHLPAAMQGLAV
                     VPLFAGYDTHAGRGRLFQYDVTGGRYEEHDHAATGSGSLHAATVVKLGFRDGLERTAV
                     IDLVLRALYEAADEDSATGGPDLLRGIFPVVATISADGFERVADDDLRTRYQTLFEQL
                     EDERSSGGASS"
     gene            complement(22554..22748)
                     /locus_tag="PLZ72_02415"
     CDS             complement(22554..22748)
                     /locus_tag="PLZ72_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HNA35181.1"
                     /translation="MAERELKKKPAPQRRSDEVEATPAATEAGEKIKAELDELLDEID
                     DVLETNADDFVKAYIQKGGQ"
     gene            complement(22907..24421)
                     /gene="dop"
                     /locus_tag="PLZ72_02420"
     CDS             complement(22907..24421)
                     /gene="dop"
                     /locus_tag="PLZ72_02420"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HNA35182.1"
                     /translation="MAIPKICGIETEYGILVRGAEESNPISASSILINSYLAALEGER
                     GAHRTQRVGWNFDDESPGNDAREEALLLSLAPEVETHLVNAVLTNGARYYVDHAHPEY
                     SCPEAPDARTALVHDRAGEEILIRSMAAARAALPEGQEIVVYKNNSDGKGNSYGCHEN
                     FLVDRAVPFGRIVAHATAHLVTRQIYTGAGKVGSEAPGMRTDEVPFQLTQRADFFEEE
                     VGLETTLKRPIINTRDEPHADAQKYRRLHVITGDANLSEVQTFLKLGTTALVLAMIED
                     DALGEPLRLARPVAAMQAVSWDLSLAEPLEMADGSRRTALDLQWDLYGRARAYAESRG
                     LEVLGPETVGEEILTRWESVLSGLDGDPMSLADQLDWVAKYRLYRAYRERDGLEWSDP
                     RLAAMDLQYHDLRPGRGLARRLGLERLTDDDEVEQAVTDAPVGTRAFFRGGCLARFAS
                     QIVAANWDSLVFDTGAPALRRVPMLEPTRGTEEHVGELLATAASAADLLDRLGR"
     gene            complement(24462..25511)
                     /locus_tag="PLZ72_02425"
     CDS             complement(24462..25511)
                     /locus_tag="PLZ72_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNA35183.1"
                     /translation="MTDTTTRDATTPEATDRPRRRHRRAGAATIALVGAIALLSGCQV
                     PLGPVTKVGVEAVRLPETSDPFVLTEGGAYYVYGSNSHVRAPVTRLTDLSRPYNLWDK
                     NAVTTNAMPTAPAWAANPNQFWAPSVARFGDHWVMYFSTDRIGAPDPANPQCVGRAIA
                     TSPMGPFVADPVPMTCGLGDHGALDPDVFTDAEGRRWLYVAFGGTETPIHVFALDGNG
                     WISGWPTAVLGRQYPWEYHFIENPSMVYDRVRRNYLLSYSAGRWYEAAYRTGIARCAT
                     PTGPCTSDPTGPWLASSNGRTGPGGLSFFTDLDGAQRAIYASFAAGLESTNGGRSASI
                     VYLRVDPSVTLSVVK"
     gene            complement(25508..26857)
                     /locus_tag="PLZ72_02430"
     CDS             complement(25508..26857)
                     /locus_tag="PLZ72_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA35184.1"
                     /translation="MDVTAVRHPAVAAEPPVDGEPGSAAEPTRRRPPWVVPVVVGVIV
                     RLLHWAVISPNWVPRSDARQYVDLARSLAAGRGFVASFPGEALHPTAFRPPLYPFLLS
                     LPMRLSGPDALWPGRLLAVGIGAALIVVTVAYARRIAGDRAGLVAGLVVAVTPSLIAN
                     DTVTMTESLGLLLMMGVLLSVLEDRFVLAGALSGLLMLTRPNAYLVVLVVVVVAVRSA
                     GWRRAAAAFGVSVAVVAPWALRNAVEVGTPKLVTSDGFTLAAMYGPPAIESGGFMDPT
                     VSSWYRDHGVWALQDDEAAWSDHLTGLAVDGIREHPASVLRRAASGAATMLEVPGFQA
                     FEAEYVDGRDPRFRDATLFTFPLLVLAGTAGVVLRIRDRRTWPALAVVAGFVALSLVT
                     VSVPRLRGPFDLLMAIGVGYLAAWWTSRGRAGAASVGSARASASAVAPADHEQGSGS"
     gene            complement(26923..28734)
                     /gene="arc"
                     /locus_tag="PLZ72_02435"
     CDS             complement(26923..28734)
                     /gene="arc"
                     /locus_tag="PLZ72_02435"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HNA35185.1"
                     /translation="MQDPDSQEPAPEDTVDDGGATDPRPPVDGTDAAALREQVRDLED
                     EVSHLVRRLQDAPKRVRTLEERLLETKGQLAQAVSQNEKLTFTLREARDHIAALRDEV
                     DKLTQPPAAYATLLRINDDGTVDVQAAGRKMRVEVSPGIDVDDLRRGNEVVLNDAYNV
                     VMVREPEVAGEIVTFKEMIDDGHRALVVGRADEERVAELADQLLGERLRAGDTVLMDP
                     RSALLLEKLPRPEVEELVLEEVPDIGYADIGGLDEQIEAITDAVELPFLHQDLFREYA
                     LPAPKGILLYGPPGCGKTLIAKAVANSLARKVTESTGKEARSYFLNIKGPELLNKYVG
                     ETERHIRLVFQRAREKAADGTPVIVFFDEMESLFRTRGTGISSDMEATIVPQLLAEID
                     GVETLKNVIVIGASNREDLIDPAILRPGRLDVKIKINRPDEAAASQIFARYLTAEVPL
                     DSDEITTLGGGDTDKAVRIMIERTVEEMYRTDEANQFLEVTYQNGDKEVMFFKDFASG
                     AMIENVVRRAKKLAIKRQLDSGARGVRVQDLIDSIHQEYKEHEDLPNTTNPDDWAKIS
                     GKKGERIVYVRTLVHDAADDDPTGGRSIEAVATGQYL"
     gene            28976..29212
                     /locus_tag="PLZ72_02440"
     CDS             28976..29212
                     /locus_tag="PLZ72_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNA35186.1"
                     /translation="MKVWIDQDLCTGDGLCEEIAPNVFTLLDDGLAYVKEGDKIFNDP
                     GGAEGMANIPDAELEGVIESAEECPGECIFIEVE"
     gene            complement(29302..29733)
                     /locus_tag="PLZ72_02445"
     CDS             complement(29302..29733)
                     /locus_tag="PLZ72_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35187.1"
                     /translation="MSDQNPPEDPTRVMPSTPAAPAPGTDPTGVPAGSAPPPGTTPPV
                     PGSGGGVPGWAWAVIALLVVLVALVAFAVLRGDDSGSTTTTSTTSSSTSTSTPTTTRP
                     TTTVRPTTTAAPTTQKPTTTSTTTSSTTTTEAPPTSEDPGA"
     gene            complement(29818..30978)
                     /locus_tag="PLZ72_02450"
     CDS             complement(29818..30978)
                     /locus_tag="PLZ72_02450"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HNA35188.1"
                     /translation="MNGLDVVIVLAAVLAAVGGWKLGFLTRSVGWIGAGMGLGLAVVL
                     LPPLLDRLDLGSSSAAVFVGLGVLVVLASLGQGIGSAIGARIAPPVRPGAARRIDQAG
                     GAVLGVVGVAVVCWLLFPVMSQTPGWVERSTDGSTLASFSLRHLPDPPAGLLELERQA
                     LDGRFPQLFTGEEPTPDLPPVPTGSPIDDATLARLTDSVVQVRGEACDMIQSGSGFVV
                     APGLVATNAHVVAGTDDVELVTPDGDRRDARVVAFDPAADLALLATSLDRPALTLDAP
                     RTGDRGLVMGFPGGGPLAPSPFEVAETLHATGLDIYDRREVRRDLLVLASDLQPGDSG
                     SAVVRSDGTVIAVAVAIAPDRDGVAYALDRSELDALLARPRGATVDTGECVS"
     gene            complement(31047..31820)
                     /locus_tag="PLZ72_02455"
     CDS             complement(31047..31820)
                     /locus_tag="PLZ72_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNA35189.1"
                     /translation="MTDPLLRAGDLVQFVDPKDRRYQATLTPGKEFHLHSGYVRHDDV
                     IGVAEGSRVETNRGQPLLVIRPTLSEFILKMPRGAQVIYPKDIGPILMLADIGPGMRV
                     LESGVGSGALSMGMLRSGADIVGYELREDFAQRAVQNVERFLGPEVLERYRVELRDCY
                     EGIDERDADRVVLDLPEPWQVVPHAEKALRPGGLILAYTPSVTQVMQLRTALEESRFF
                     AAATTEVLQRGWYVEGQAVRPDHRMVAHTGFLTVARLGT"
     gene            complement(31817..32830)
                     /locus_tag="PLZ72_02460"
     CDS             complement(31817..32830)
                     /locus_tag="PLZ72_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35190.1"
                     /translation="MKCRVCRGPAVIDLPRHNANFCPEHFTRFCRDQVERAISDFDMM
                     QPEDRILVAVSGGKDSLAVWDLLLGLGYAADGLYIGLGIGDYSDTSGGYARRFAQQHA
                     EPRGAKLIEIDLVDDYGYDIPGGARAARRTPCSACGLSKRHLFDRAALDGGYDVLVTG
                     HNLDDEAAVLFGNVLRWQTDYLGRQRPVLPARDGFPRKVKPLVRLSERETAAYCIVSG
                     IDYLVDECPMSAGNKHLGYKEALNEIEVRSPGSKADFYFSFLRRAVDRFAPEPGTGDA
                     DRADPGVLAPCARCGSPTSTEVCAFCRLVERAGGSPPDAPPARPVTLGRRGGPDPTGT
                     PSP"
     gene            complement(32827..33027)
                     /locus_tag="PLZ72_02465"
     CDS             complement(32827..33027)
                     /locus_tag="PLZ72_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNA35191.1"
                     /translation="MLVRLRNPNREVHADAPVLVSVLLERLDINRESVLVVRNGTIVP
                     GDERLEPDDVVEVRPVISGGSA"
     gene            33089..33988
                     /locus_tag="PLZ72_02470"
     CDS             33089..33988
                     /locus_tag="PLZ72_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35192.1"
                     /translation="MTLWNDPQIESWQARGRRVTVSGPALPTQDVFVVDVAAADPDPA
                     LEVLLVVHGFPTSSFDWAHLVDDMARHRRVVLVDLPGFGLSDKPDRHYTIAGAADAVA
                     GVTSALALDRVALLTHDMGDTVGGELLARQMEGTWPVEITRRVVTNGSIYIDMAQLTD
                     GQLMLLSLPDERGPAGPGPELLAASLQATLAEAHRAGTDMTAAAELVCHDGGDALLPR
                     TIRYIEERRSNERRFTGAIETHPSPLHVLWGPEDPIAVPSMTDRLLEARPDATRTWID
                     GAGHYPQNEDPAAYLAALEQALG"
BASE COUNT         4686 a        11700 c        12562 g         4890 t
ORIGIN      
        1 tccgcaggcc gccacgctca tgcagcagat cgcccaggcc atgaccttcg gcctgaccgc
       61 cgaacaggtc gcccgcgagc agatctggaa ccacccgggt ctgcccgaag tgctggagaa
      121 cgccctcctc gacgccttgg aggcggccgg atcagggggc tgagtcgagc accagatcca
      181 cggccgcggc gaacgagtcg gcctgttccg tcggcggctc gagaccatcg ggccaggtgc
      241 ggccccggcg gagccagacg ctgcggatcc cggcagaccg ggcggcctgg acgtcgttga
      301 gcgggtcgtc gccgatcatc cacgatccct cgagagaggc cccggcccgc tcggcggcga
      361 tgcgcagcaa ccccgggtcg ggcttgcgga ccccgtcgat cgccgacacc gcgacggcgt
      421 cgacgtaggc cgagaggccg accacggcga tcttgtcgag ctggggctgc tcgccgttgc
      481 tggcgatggc gatcgtccaa ccggcaaggc gggcacgcct gagcgccgct gcgttgtcgg
      541 gttccagcgt gaagcaggcg acgtagtcgt gcaggtagtc gagggtcagc ggcggctcca
      601 gccccaggcg gtggttgacc gcctcgacga actgggggcg cgtccgggcc ccgtggtggt
      661 cctcgtcgag gatcgcccgg accgcaccgg agggcaggcc gcgatcgcgg gcgaacccgg
      721 cggcccagcg ctcgagcgcc tctcgtcgtt cgaggatcgt ctcgtcgagg tcgcacagca
      781 gcaggggcac gccctgttct gtagtgcgag atccaggcga acggctggat ctcgcactac
      841 agaacggcgg atcgggctac gtgggtcgcc ggggcgtccg ttagcgtggc cgccatgtcc
      901 cgccgactcg agatcgagct gacctccgaa cgccccgatg gaacctggac ctggcgcgcc
      961 gccggagcca agctgcccaa gggcgacctg agcggctcgt tgctgccggg aggctccaag
     1021 gtcggcgacg tgctgcgggc cgaggccgag ttcctcatcg acggcatcga catcgtcgag
     1081 gtgctggcac cgaaggggac gcgcaagcag ccggagctgc tcgagatcat gggttcgggc
     1141 cgcgaggagc cgctggtcac cacccagctg gtgtcgcgcg gtcgcggtga gggccggggc
     1201 cgtcgacgcg atcgcgacgg tgacggaccc cgtcgggagc gccgtgaccg tggcgaccgg
     1261 cccgaccggc cgcgggggcc gcgtcccgag gtccccgagc gacccaagcc caagcggctg
     1321 cggcccggtc gagcgcaccg caacgcggtg atcgccgccg ttcccgagga gcagcgcgcc
     1381 atcgccgagc aggtgctccg tggcggggtc cccgccgtgc gcgaggccgt ggtggcccag
     1441 aacgagcagg ccaggaccga gaaccggccc gagcagcccg tcgatcaggt cgtggccatg
     1501 gccgagagcc tgtacccccg gctgcgcgac gccgagtgga aggaccgggc agccgccgcg
     1561 atcgcggatc tcgacgagct cgacctgcgc gatctccgct cggtggtggt ggccggtgac
     1621 ggggcctccc acgacgacga ggcccgcgcc atgctcgagg agctgcggac cggtctgacc
     1681 cgccgtgtcg acgaggagca cgccaactgg gtggccgaca tcgccgccaa cctcgacgcg
     1741 ggacgattcg tgcgggccct gaggctcagc tcccgtccac ccaaggccgg cacgccggtc
     1801 ccgaccgagc tgtcggcccg actggtcaca gcggtgtccg agggcctgac ccccggcacc
     1861 aaccaggagc tgtgggccgc ggcgctcgac gccctggcct tctctcccgt acgccagcag
     1921 gtgactccgg ccgggcgacc cgagaccccc accgacgacc tgctcgaggc tgttcgtcgg
     1981 gttgcggacc gggtccccgc catcgccgcg ctgttcggtg tcgatccctc cgaggccgcc
     2041 gcggccaagc gtcgtcgccc gccccgcggc cgttccggcg ccggtcgcgg cgcgaagccc
     2101 ggtgacaagt cgtcgaccgg ttccggcaag acagcggacg acaagacagc ggacgacgca
     2161 gcatcggacg acgcggcatc cgcagggacc gctgatgccg aagcggtcgt cgaggtcgca
     2221 gaggcggaga ccgcccccac cgagccggtt caggccgagt cagaccaggc gaagtaagac
     2281 caggccggat cggcgagctc cgaggactga cgtcggtcag ccgtagaggt cgagcagctg
     2341 ctcgggccga tcgagcaccc gtccgtgggg gcgggccgcc cggacccggg ggttccaccc
     2401 cgcccacacg taccggcacc cgaccgcctc ggcgacccgg acgtctccgg cgctgtcacc
     2461 gaccatcacc acctggtcgg gtgtgagagc cagtgcgtcg aggaccgggc cgagctcctt
     2521 gtgggcccag tcgaagctgt cggcgaacag cgccacctcg ggattccagc ccaggcgagc
     2581 cagctcggag cgggccggtc gggggtgctt gttcgagcac aacgcccacc ggccgagctg
     2641 tccgatgacg tcgtcgacgc cggggaacgg ctgtgcggcg gagtcgtcgt agaggccgac
     2701 gtaggactcc atcgggatcc cgagtcgctc gcactcctcg gccaccgcgt gtccgaaggt
     2761 gatctcctcg gtgggcacgc ccaatgccag cagcggtcgg atcagggcgt cgtcggagtc
     2821 gacgagcgtc ccgtcgaagt cgaacacgag ggcgtcgatg gcggtcatca gcggcgcacc
     2881 ctagcgatca cccgcgttga cccggagacc cccgggcgga tcccgcggcg cggagtagac
     2941 cggtcggtca agtgggagtc cggccgggcg gtaacgtggc ggaccatgac catcgagtac
     3001 accaagcagg gtaacgtcgg gatcatcacg atcaaccggc ccgatgcccg caacgccgtc
     3061 aacagcgacg tcgcccaggg catcgaggac gccatcgacc agatcgaggc cgacgacgag
     3121 gtgtgggtcg gcgtcctgac cggggccaag accgacaagg gctacatctt ctgcgcgggc
     3181 gccgacctca agcagatggc ggtcgatccg ggtggcatgc agaccgcccg gggcggcttc
     3241 gccggcttcg tcacccgtga gcgcaacacc ccgatcatcg ccgcggtcga cggcccggcc
     3301 ctggccggcg gcaccgagat cgtgctcgcg gccgacatgg tggtggcgtc gcggaccgcc
     3361 gtgttcggca tccccgaggt gaagcgcaac ctggtcgctg ccgccggcgg gctcttccgc
     3421 ctgccccgca agatcccccg caacgtcgcc atggagctgg cgctgaccgg ccgtctcgac
     3481 ttccccgccg agcgcgccta cgacttcggg atggtcaacc gactctgcga cgagggtcag
     3541 gcgctcgaga cggcgctggg tctggccggg gagatcaccg agaacgctcc gttggcggtc
     3601 gccgagagcc gacggatcat gatcgaggcc accgaccagc ccgacgaggt gggttggaag
     3661 ctctccggcg agggcatcat caagatgttc ggtaccgagg acttctccga gggcctcacc
     3721 gccttcgccg agaagcgcaa gcccaactgg aagggtcgct gatccacgcc cgctgagcgc
     3781 gggtcacccc ggggccactc gacgcccagg ggtgacccgc ggttcaggcc agagnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccccg gtccgactcc gcgggtagga
     3961 cctcgtacca gccgaggaac gcgccgcaca gcgcgtcgag ccccgtgagg gagcgcacgg
     4021 cgcgcaccac cgcctcgacc atggtctcgc cgccccgcac cggggcgctc ggcaccgacc
     4081 agcccctctc ccctccccca tcggccgagc ggaccatgag caggtcgttg ccgtgggcca
     4141 ccacggccgc ggcccgcagg accggtggtg gttcctcagc cacggctgcc gtcggggctc
     4201 cgctccccca acgaccggtg cacggtgatc ttgcgggcga ccagtccgaa tgcctcgaag
     4261 aagttcttcg tggagcggtc gcccggcagg acctcggcgt cgacgccgtg gcacccggag
     4321 tcggtggccc actcgacgac cgcttccatc agggcccgtc cgacgccgac gccccgggcg
     4381 tcgggctcga cgaacaggtc ggagacgacc gccagtcggc cgttcacggt ctcctccagg
     4441 cggaccacgg cgtagccgac cgccaccggt cccaaaccgc cgacgagcac gagggcggaa
     4501 cccgcgtcgg cggcggcgag gtcggcgtcg agggagtcac ggagcggatc ggggcggccc
     4561 tcccgctcct ggtagacgtc gccaccgcga tggtgtccca cgtgggatcg agcggcctca
     4621 ccgaggcgca ccagctcgcc gcggtccagg gggcgggcac gccggacgcc cggttcgaac
     4681 ggggcgggac cagagggctc ttcgcccatc gggtcaggag ccgttcgtcg cggcctgcag
     4741 ctgccgcacc cggttcagga tcgtctggcg ggaccggtgg ctctgctcgt aggtgagcac
     4801 gagggcgagc tcggcggggt cgagcgacgc caggcggggt acgacctgtg acgcagccag
     4861 gctgtcgtag tcggggatgg ccaggtcctg cacgtccacg gtcgggagtt ggtcacggac
     4921 gtcggcctcg acggcggtcg gagcgggttc ggggtgatcc gccccgtcgg cgccctcacg
     4981 gtcgtcccga tcggagtcct caggggtgcg agcggatcgg tccccggccg cggagcccgg
     5041 ttgtccccac aggttggaga ggcacgggag ttgccccacc agccgctggg tcagctcgac
     5101 ctgggcgcgg gtgcgttcga cgagggccgg gaccgcctcg gacacctctc gggccagcag
     5161 cgtggggatc tggagggggt tcacagcgcg gcgtcagccc ttcttggccc gggccggggc
     5221 cttggtgcct cgggtgagga tctcgatggc gccgcacggg tcgtagccct cgggacagct
     5281 cagcgccccg ctgcgcccga actggcgggg gtgcacgatc aggaagcacg tggggcaggt
     5341 gaactcgtcc gcggtcttgg ccgccacccg ctcggtgctc tcggtgccgg gctcctcgag
     5401 ctcgtcctcg tcgtcctcgt cggcgcccgc cgcgatccgg tccttgagga tggtgtcgag
     5461 atcggcctcg acgtcgtcgg gatcgacgtc ctcgtcgtcc tcgtcgtcgt catcggccga
     5521 cgaggggcgc ttggccttgg ctgccgggac gtcgtcctcg tcgtcctcgc cttcgacttg
     5581 gtcagcgtcc tcctcgccgt cgaggtcgac gtcgtcgatc tcgtcgtcga tctcgtcgtc
     5641 gagggcgtcc tcgtcctcga ggccctcctc ctcgtcgagt tcctcttcca ggagttcgtc
     5701 gtcgtccatg tcgctgtgac cgtgtccttc cggcctagtt cctgctggcc tgtggctgcg
     5761 ggcttctcgg tgggggctcg tggggtggaa cccggttgtc gggtgccctc cgggtccgcc
     5821 tctccgcggc ctcgtggtga ccgtcgagcc cggttcgggt cgaggtcggg gcgcatcata
     5881 gacacggcgc cccgctcagg agtaccggat cggccgtcgg gccgccacga gcgtcggctc
     5941 aggccgattc gctgcgtcgt gcctcggccc ggcgttcggc tcccaggcgc tcgagctcgg
     6001 cttcgccgga gtggtccacg aaccgcatca tctcggccac tgcgcggtgc ccggcccgct
     6061 ccgcgatcag gtggtgcatg cgctggccga tcgcccgata gctcggatgc ccctgcaccg
     6121 aggtgcgcag ctcgatcagg tgcatggcct cgcgggcgtt gaactgcatg acgtagcgga
     6181 tccggtacgc gagggacacc gcgtaggacg ctcgctcggg gaaccgctcg gcgaggaccg
     6241 cccagagccc ggaggagcgc tccatggcct cgtcgaagcg agcggtcatc ccggcctcgt
     6301 cgatggcggc cgggcgggtg aagccgtggg ccggcgtcag gtcctgccac tcgatcgtca
     6361 tcaggcggtg tcgttgcagg tcccggaacg cgccgtagtc cgacaccacg tcgaagcggt
     6421 agtcggtgcg ctccagcgcc cggcccggct tgtggcggcg gttggtccgg tccccgacgt
     6481 aggcgcgcat gaccgccagt cggtcctcgg tcgacatggt ccggacccgc tcgatcagcg
     6541 tggactcggc cagacccgtg tacgggtaga gcatggcggc gacgaccttg agctcgccct
     6601 cggggtcgaa gtcgacgagc tcgacgcccg gatcggtggg accgtccacg ccgtcgccgg
     6661 acagcatctc gtaggcgacc gcctccatgg cctcgcggtt ggagcgcagg tactcggagc
     6721 tggtgacgcc ccggtcgggc agatccactc gcttgaggaa gctggggacc accttgcgca
     6781 gctcgaccag catcaggtcc gcgtactggc gggcctcggg cagggggtgg ctgcgcatgc
     6841 gcagcaacag ggcctcgaag gcctggcccg aaccgtagat cccgacgttg gacaacgacg
     6901 cagcgggcag gatgccgcgg accgagtcga aggccttggc ccggatggcc tggcggtaga
     6961 cgaagtccga gtcggtctcg ctcttggggt ggcggtcccg gaagtgctcc gacatgtcgg
     7021 tggccagtgc cgagtacgag tcgaacagct ggtccatgtc gccgacgtag cgggctccga
     7081 gcggcgaacc gaggatctcc gggtcgcggt ggtagcggta gcgaccgccg agtcgctcgt
     7141 cgtaggggat gtagcgcgtc gactgctcca ggtaggacat cagccgaccc cactcgagca
     7201 ccttggtcag gaggttggac gcctgctcgc acgcgaggtg cacgccaccg agctgagcga
     7261 cgctgtcgtc gccgtactcg aagaagacac ggtcgtagag cgactcggcc cgctcgaggc
     7321 cgatggtcgc gtcgacggtc tcgtcgccgg tggtgtcgag ctcgccgacg aactcgtcga
     7381 ggaagagtcg ccgcagggac ttggccgaac gcgagtaccg ggcgaacagg gctcccttga
     7441 ccacctcggg caggttcacc agggcgaaga ccgggccgtc caggctcgtg aagtaccggc
     7501 ggaggaccgc ggcctcctca ggggtgaact gctcagcgac gtaggtcggg agtgcggtca
     7561 tcggaccgtg aggatagagc cgccgggcgg cagtcccggg gaccggtctc agcgccgcga
     7621 accggcaggg gaaccggcgg cccaacgatc gaggacgacc ccggccaggg ccttggcccc
     7681 gtcgagcacc gcggcgtcac ctccggcgcc gcgggcgtgg gtggcgaact ccgcggtgtg
     7741 gatcgccacg ccggggggcg ccacggcgat catcgggtgg atcgacggta cgacgtggct
     7801 gacgttgccc atgtcggtgc tcccgacgac ggggcccacc gtgtcgggat cctgcggcgg
     7861 ccggccgacc gcggtgctgt gacggaccca gctgtccagg agccagcgat cgtcgaccag
     7921 gtcgtcgtag gcgggatcca accactcgtg gtccatccga cagccggcgg ccgacgctcc
     7981 tgcggccagg caggccagca cccgttcctt cagctcggcc aggccctcga cgctcgggga
     8041 gcgcacgtac cacgacgcag ccgcgcgccg gggtacgacg ttggcctgct cccccgcctc
     8101 ggtgaagatg ccgtggatcc gttcgtcggg ccggatgtgc tggcggagag cggccacgtt
     8161 gacgtagccg agcacggcgg cgtcgagggc gttgaccccg ttccacggcg cggcggcggc
     8221 gtggctggcc cgaccggtgt aggtcacgtc cagacgctgg atggcgatcg ccgacatggt
     8281 cggcaggtcg tggtcggccg ggtgcaccat caccgcggcg tcgatgtcgt cgaaggcgcc
     8341 gtcgcgcaac atgaacacct tgcccccgcc accctcttcg gccggggtgc cgatcacccg
     8401 cacccggccg cccagcgcgt cggcgagctc ggcggcggcg aggcccgcgc ccaggccggc
     8461 ggccgcgatg acgttgtgac cgcaggcgtg gccgacgccg ggcagggcgt cgtactcgca
     8521 gatcaccgcg acggtcgggc cttcggagcc ggcctcggcg acgaacgcgg tgtcgaggcc
     8581 gtgggcgtgg cgggtgacgg agagcccctc ggcctcgagc gcgtcgcaga gcaggtcgtg
     8641 ggcgtggtgc tcgtcgaagg cgagctcggg ccggtcgaag atggcgtggc tgatcgacag
     8701 gagccggtcg gcccgtcggt cgaccgcggc gaacgcggcg tccacggcgt cgggcgacgc
     8761 cgtcggggtc gtgtcggccg cggggtcctg agcggtgtgg tcgggacggg ccgtgttcac
     8821 cgcgccaccc tactggcggt cgatcacggc tcgggatgct cgaggacgat cttgccgagc
     8881 tgctcggcgc tgcggagccg ctccagagcc cgcgggtact ccgagagggg gaggatctcg
     8941 tcgacgacga ccggcaggcc gtcgcccagg agatcggtga cgtggcggaa ctcgctctgg
     9001 ctgccgcagc tcgctccgat gacgtcgatc tgcttgaaga acaaccgcgg gaggttgagc
     9061 tcgaccttgg ggcccgacgt gccgccgcag acgcacatcc gtccgccgtg gcgcagcgtg
     9121 cggaaggcga agtcccaggt ggcgggcccg atgctgtcga cgacgatgtc ggcggtgatc
     9181 gggtacggct cgtcggaggg gaacgcgccc tcggcgccga gctcgatcgc ccgatcccgc
     9241 ttggcggcgt cccgggaggt gacgaacacg cgggcgccga cgtggcgggc cagcatcatc
     9301 gccgcggtcg cgacgccgcc gccgatgccg gtgacgagca cggtgtcctc gggttgcagc
     9361 cgggcccgtc gcagcagtcg ccacgcggtc gtgtagcaga ccgggtagct ggcggactcg
     9421 gcccagctgc ggcctgcggg acgccgttcg agctggtggc cggggacgac gcagaactcg
     9481 ccgtggccgc cccagcagct ctctcccagc agcatcatgg ccgggtcgag gacgctgtcg
     9541 tcgccgcgtg cgagtgcatc ggcgggcacg accgcggtgt tgatgacgac ctcgtcgccg
     9601 ggcacccagt cggtcaccct ctccccgacg gcgtcgacga cgccggcgac gtcgttgccg
     9661 gggacgtggg ggaacctcgg cggcttgggc agaccggtgg tcagccacag gtccatgtgg
     9721 ttgagggcgg tcgccctgac ccgcacccgg acgtcggcgg gtccgacctc ggggtcgggg
     9781 acctcgcccc actggtatcg accgggggac tcgtccaggt accacgcgcg catcctcacg
     9841 aggctaccgg tcccgatcga gccttcagac gacgatgacc agggcgtcgg gaaggcagcg
     9901 cagctcgaag tgggtggcgg cgccgatgag ccgaccgtcg acccggatcg gcagctcgcg
     9961 ctcgtcgagg acctcgtagg cggcggtgcg ccgctcgagg atccccggat gcgggacgtg
    10021 gctgccggtg cgctcccgtc ggccggctgc ccggacctgg ctggcgggca cccgcccgtc
    10081 gctgatgtcg aggagtccgt cgttcggatg ggcgcggggg ccgaggtccg cgccggccag
    10141 gtgcgttccg ttcatggcga ccaccgtgcg tgacgaccac cgtcgtcgac cccggccggc
    10201 ggtggcatgg gccacgaaca cgtgccggga gatcaggccg tcgccgcggt cgacgaccac
    10261 ctcacccagg tcgatcggca ggcgcatccc cgccccggag cgcaggtcgg cctcggtgtg
    10321 gcccggtgcg ccgagcgcgg cgtggaggtc ccctcccaac aggccgacct cggtgacggg
    10381 gcgtccggct gccagcccgg cggcgaccag gtcggcgagc tcgccgtcgc cggccgcgac
    10441 gggcgcgtcg gctgccagcg gcccggctgc tccccacggc tccccccggc ggatcgtcac
    10501 gacccgccgc ccggggtcac gtcggaaccg ccggagctga cggtgcttcc ggcctcgacc
    10561 acgccgcgga acaggctgtc ggcggcccgg cggcacaccg ccgcggtccg cgggtcggcg
    10621 gcgacctcgc cgagctgccg catcaggtcg atcagctgct tggtgttgcg gacgaagtcg
    10681 cctccggtca ggtcgtcgtc gagcactgcg gcgaggggtc gcccggcggc ccaacggtgg
    10741 gcggtggccg cgaagcccgc gtcgggggcg cgggtctcgg ggacccgggc ggcccgctcc
    10801 cagccgttga ggcgcgagtg gatctgctcg agccgcttga agcgacgccg cagctccgga
    10861 ctcgggtgcg tcggaggcgg cggggcgtcg ggactgcggt gctcgtaggt gaagcagctg
    10921 accatggcgg ccatcgccgg ggcgtcgagg tcggcgagca ggccgtcgtc gagcgcgagc
    10981 gagatgagca ggtcgcactc gtggtagatc cgccgcaggc gctccccgga gtcggtgagc
    11041 gaccatccgc tcgtgcgacc cgagcgcgcc agcacctcga gcacggcgtc gaatcggttg
    11101 acgagccccg aaccacgccg ggcgagccga cgacgtgcca cctcgaggtc gtgggcgagg
    11161 cgggcccgtt cccggtgtcg ggcgaccacg gcgtcccggt cgtcgtcgtg gtgcagggga
    11221 tgcgactcga gcgcagcccg cgctgccagg tgtgcctcga gcacggcggg ctcgacgtcg
    11281 tcgaggtggt cgtcgtggcg tcgtcgtccc ttggccgccc tgccccgggc gccgcgcagg
    11341 ttggtgcgcc gcagggcggc cgcggcgtca cgtcggtagg ccgaccgtcc cggctcgaac
    11401 ggcaccggga ggtccacccg ggcgatgggc cacaccggcg aggccagatc tccggcggtg
    11461 agctccttgt cggccccgtt ggctgccacg gtccgcaccc gcacggcccc acccttgcgg
    11521 aacgccacgg acagcacgag caggggctgt tccggccgac ccggatcacg atgcggcacc
    11581 gacacgacgt cgccggggct cagcgccgcg agcgaggcgc cgatgcgctc ccgtcggtcc
    11641 tggggtcgag gggtcgtggc tgccagggtg tcgagggccg cgacgtagtc ggccacagct
    11701 cggtcggcgt cgccgtcggg tgggccgccg agcaggtcga gctcggagcg gatgcggtcg
    11761 agccgggtct ggagcgtcac cgcggcacgg tcggcctgga actgggcgaa ggagcgggcg
    11821 agcacggcgt gggcgtcgtc gcggtcgtag cggtgcacca ggttggccgc catgttgtag
    11881 gtggggcgga acgcagaggt gagcgggaac tcccgactgg cgacgagccg tgcgatctgg
    11941 gtgaaggtga cgaacggcga ccacaggacc accgcggtgc cctggtcgtc gatcccccgg
    12001 cggccggcgc ggccggtcag ctgggtgaac tgcgcggggg tcaggaactc gtgggtgtcg
    12061 ccgttgtact tggtcagctt ctcgatcacc accgaccggg ccggcatgtt gatgccgagg
    12121 gccagggtct cggtggcgaa gacgaccttg atcagcccct cgttgaagca gtcctcgacg
    12181 gcctcccgga acgccggcac catcccggcg tggtgggcgg ccacccccgc ttcgagggtg
    12241 tcgagccact ggtcgtagtc gagagcgtcg agatcggagt cgctcagcgt cgcgacacgc
    12301 gcctcgacca gggcccggat ccgggagcgt tcggcggcgt cggtcaggcg cagccccgcg
    12361 tcccggcagt gccgggccgc gtcggtgcag gcggcccggc tgaagatgaa gtagatcgcc
    12421 ggcaggaggc cctcgtcgtc gaggcgttcg gccacctcga gccggcgggg cgtgcggaag
    12481 cgacggccgc cccggggccg gccaccccgt ggccggccgg acccgcctcg gtggcgtccg
    12541 gcgccggagc gctccgcgtc gaagcggtcg gcctcggggt tgggtcggcc gtcgacgagg
    12601 accgggagca ggtggtcgcg ctccgccgag cggtcgccga ccatgtagag gttgtcgagt
    12661 cggatcggcc ggacgtgctc gatcacggtg tcggtgggtc cgcggagggc ctcgatccac
    12721 tcacccagct ccgccgcgtt ggacaccgtc gcggacaggc acacgaagcg gaccgccgcc
    12781 ggggtgtgga ccaggacctc ctcccagacc ggcccccggt acgcgtcctg gaggaagtgc
    12841 acctcgtcga gcacgaccca ctgcaggccg tccagcgccg gcgaaccggc gtagaccatg
    12901 ttgcgcagca cctcggtggt catgaccacc accggagcgt ccgcgttgac ggtgtggtcg
    12961 ccggtgagga ggcccacccg gcccgggccg agcacggcga ccagatccgc gtacttctgg
    13021 ttggacagcg ccttgatcgg ggcggtgtag aaggcctttg cgccaacggc cagagcccgg
    13081 gcgacggcgt gctcaccgac gacggtcttg cccgacccgg tcggggcgga caccaggacg
    13141 ttgcgtccgg cgtcgatgcc ctcgatcgcc tcgcgctgga agcggtcgag ggggaagtcg
    13201 tgggcgaagg gatcggtcag gacccgtctc cgtcggtcgt gtcgggctcg gtcnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgacggg cggcccgggc ggcccgccgc
    13381 tccttctccg cctcggcctc acggctggcg gccttgcgct tgcggcggtt ccagagacgc
    13441 ccgatcacga tgctgatctc gtagaagagg tacatcggga tggccagggc gaacatcgag
    13501 atcggatcgc cgctcggggt gatccccgcg gcgatgatcg tgatcagcac gatcgcctgg
    13561 cgtcgccacg acgacagccg ttcaggggtg acgacgccga tcatctgcag ggcgacgacc
    13621 accaccggga actggaaccc caccccgaac gccaacatca tgaacaacgc cagcttgacg
    13681 aagttcccga tgcccgagcg gatctcgacg tcgggtccgc cgatgctgac caggaagttc
    13741 accgcctggg tgagggtcag gtaggcggtg acggcgccgg cgacgaacag caccatggcc
    13801 gacacggtga aggccagggc gtatcgccgc tccttcttgt agaggccggg ggcgatgaat
    13861 cgccacagct gccacaggat caccggcatc gccaggaaga gcgccccgta gccggcgatg
    13921 cgcagacgaa gcgtgaacgg gtcgagcagg ttggtctcga ggaacttgca gctggcgtcg
    13981 gggtcgaggt tggtcagcgc gtcgcagtac ggcgcgttga ggacgtcgat catccacggg
    14041 tacaggaacc acgccgggat gagcatggcc gcgaccgtgc ccacgcagat gagcaggcgt
    14101 cgacgcagct cgcgcaggtg atcgcccagc gccatcgagg cgccctctcg ttcggctccc
    14161 ttcaggacgg ccacggtcag gcggtctccc gctccccggt gtcgtcgctg gagccgtccc
    14221 cggagtcgtc cccggtggaa gcatcctcag ccgggtcggt ccccgtcggt tcggccgcag
    14281 cccccgattc ggtggccgcg tcgatcgagg cgcgttcgct ccgggcccgt tcgtcggcct
    14341 cggcctcgag cgcgtactcg gcgagcttct ggcggggtcg ggccgccagg tccccgagct
    14401 cgcgcagcgg ttgcatggcc ggatcgtcca tgacgccctg cacctgcccc tggaggcccg
    14461 aggacatggt cttgagcttg gcgatcatct tcccggcgct cttggccatc tcggggatgc
    14521 gctcgggtcc gagcacgacc agggccagga tcaggatgac gagcagctca ccgccgccga
    14581 agttctggat catcggcccg ggtggaaccg gggctccgag gcccgtgacg acatggcgct
    14641 cagcgtaccg gtcggggccg gggtgtcggc caccgccgtc ccggcccgct cagcggtcgt
    14701 tgatcgcggt gagggtggtg aaccagtccg gggtggagag caggtcgtgg aagtcgagca
    14761 cgtcgtcgtg ggtgatcggt gcgcctccgc ggcgctcggt cagctcggcg ggcaggtgcc
    14821 actcggtgct gtgcaccccg gcggcgagga ggacgtcgac gacgtgctgc tcggcgggac
    14881 gcacctcgat catgcggcac accggacagc ggaacaggta cgtcgccgac tcgtcgtcgc
    14941 ggcactggcg gacccgcagg tcccgggagc ggagctccac gtcgccgcag tcgctgcatg
    15001 tcgctcggat gagtgccatc gtcgggtgtc tcctcgctga ggtgctccac atctcttcgg
    15061 tccggacccc gttcaggttg aaaactccgg ctcaggaatc tcgcgacctg accgatggtc
    15121 agcgacgacg acgatcggcc ggaacgggct gcgggcgtgc cacgatggcc gctgcatggc
    15181 cgagcgactc ccctccctcg tggacccgcc gatcctcttc gcacaccgtg gggcccgcgc
    15241 ccatgccccc gagaacacca tcgaggcgtt caccctcgcc ctgcgactgg gcgccaccgg
    15301 cgtggagagc gacgtgtggg cgaccgcgga cggcgccgcg gtgctggacc acgacggtct
    15361 ggtgcgacgg ggcctgcgtc gcagcgccat cggcggcttg ccccgggccg atctccctga
    15421 gggcatcccc acgctcgacg agatgtacga ggcgatcgga tccgacgtcc aggtctcact
    15481 cgacgtcaag gaccccgacg tgctcgacga ggtcctggcc gtcgcccgcc gggccggggc
    15541 ctcggagaac ctgtggctgt gccacccgga tctcgacctg ctggccgact ggaagcagcg
    15601 gtgcggagcg gtgcgcctgg tgcactccac gcggctgggg gctctcggcg ggaagccgga
    15661 accccatgcg gcgcggctgc gcgagctcgg catcgacgcg gtgaacctcc accactcgga
    15721 gtggtccggg gggatggtcg ccctctacca ccgcttccgg agggtggcgt tcggatggga
    15781 cgcccagcac gagcgggtga tccggtcgct gctgggcacc ggcatcgacg gggtgttctc
    15841 cgatcacgtc gaccgcctgg tcgacgtggc cggcgaggga tccgaacccg gaccgacgtg
    15901 gtgatccccg tcgacccagg acgctagaga gacccgatgc gcgcgggtgg ccacatgcgc
    15961 atgaacgccc gccccacgat cgagtcctcg ggaacgggcc cgaagacccg gctgtcggcc
    16021 gagtcgccgc ggttgtcgcc catcacccag acgtggccct cgggcaccgt caccggctca
    16081 tccatgccat cggtgacgac ccccttctgc aggtacggct cctcgagcag acgaccgttg
    16141 acgtagacct tgccgtcgcg ggactgcaca gtgtcgccgg gccgcccgat cacccgcttg
    16201 atcaggtcct tggggtcgtc ggggccgccg gagagggatg cggggcgggt gaagacgatg
    16261 acgtcgcccc ggttgacgtc gtggaggtcg tagctcagct tgttgaccag cacccggtcc
    16321 cccttgacca acgtcatctc catggatggc gacgggatct cgaaggcctg gaccaggaag
    16381 gtcttcacca ggaaggcgac caggatggcg ccgacgatga ccaccgccca ctcgaccatg
    16441 ttccgggtcg aggatcgcga gcgggtccgg gcggcgggcc ggcccgacgc tgcgggctga
    16501 tcgacggtcg gctcggacat cgatgggagg ctatcggtgc tcagcggtac cgccgcagca
    16561 cccgtcgggc cgcggtggcg cccagcggtc gcaggtcctc gccggtgtcg acgtcgcgcg
    16621 cctcgaccgt ggggccgagg cgcagcagca accgctgcag ccagggctcg gcggtgaccc
    16681 gcagcacgac ccggatccgg ccgtcggcgt cggtggtcac cgactcggtc ggcaccgcgt
    16741 cgacgatcca ctcgtcctcg gcgccgacga ccagcgccac cggtcggccg tcgctcaggt
    16801 cgagcacgtc ggcctgttcg acgttcgggt ggtcgtcgac cgcgccgccg gtcggcaccg
    16861 cggtgtcgat gcggtcgacc cggaactccc ggacccctcc ggcgcggtgg caccaggccc
    16921 gcaggtacca gtggccgtcg cgggccgaca ggcgcagcgg gtccacctcg cgctcggtgc
    16981 gctcgtcgcg tccgaacgag tagtagtcga tccgcaggcg ctgcccggcg tcggtggctc
    17041 ggcgcacggc gtcgagcacg tcgggatcgg cgtcgccgag gcgtacgtcg acggagtcgt
    17101 cggctccgtc gccgaggacg gcggcgagct tggccacggc ccgtgccagc accgggtccg
    17161 ggtcgggtct gtcgagcagc gcttgacccg ccgcgtacag gaccagggcc tcgtcggggg
    17221 tcagccgcag cggacgtcgg aagtagtcgc cgaggtggat ctggatccgc ccgtcgtcct
    17281 cgacgtaggc gtcgacgcgg gcatcggggg tgaacgggtg gatgccgacc tcgtacatga
    17341 gcaggtcgag gtcggcccgc aggtcccgct cggtcatgcc gaagcggtcg cagacctgct
    17401 ggacggtggt tccgggctcc tcgacgatcc acggcaggac cgacaggatc cgctcgacgc
    17461 gctcggacgc ggtggcggga ctcatgacga cagcctctcg aacccgccca gccagtcgat
    17521 gaggtcgcgg cggtgcccgg gcgggtcgag cagttcggcg tggtccaggt gggacagcac
    17581 gaagctgcgc aggccggcgg ggttgcggac ctcgagctcg atcacgaccg atccgtcggg
    17641 ctgggtggcg ctgaccgtca gctcgggatc ctcggccagg accgaggcgg ccaccgtggg
    17701 ctcgagcagt acgtgcgcgg tctcggccgg tcccgggccg agctcccacg gccgcaggcg
    17761 caccccgggg aggtcgtcgg gtggggtgaa cgcaccggcg tcgccggcgg tgacgggacc
    17821 cgcgacccgg tccagtcgga agctgcgggt ggcgtcgcgg tccaggtcgt ggccgctcac
    17881 gtaccactgc ccctgctcgt actgcagccg gtggggctcg aggtgccgct cgtcgccgga
    17941 atagccgaac gtgatgaccg cgtggtcgag cacggcagcg aacaccacgg ccagggcgtc
    18001 gggtgcgtcg agcgagccga ccggtgctgc ggcccctccc ccgccggtcc cgccgagctt
    18061 gcgcagtgcg tcctcgacct cgtcgacggg gatgccgacc agccggaccg aggtcgccgc
    18121 caggtggagg gccgccagct cctcggggct caaccccggg tcggccagct cgtagcggtc
    18181 ccggcggatc cggtaggccg cccgtggttg ctcggtgtgg gcgaccatca ccgtctcgac
    18241 ggggacgccg gcctggcgca gctcgtcctt gtcgcgctcg aaggtccgcc ggaacgtgtc
    18301 gtcggagtgc gactcgtagc ccgggacccg ttcgcggatc tcctcggcgg tgagcggcac
    18361 gctggaggcc tgcagtgcgg cgacgatgtt gaggaggcgg tcgaccttgg ccacggcggg
    18421 tcagtgtacg ggccgatccg cggatcgtgg cgtgaccggg gccgtggcgc gatcggccac
    18481 caggtccgcg gccaacgcac ctgccgcgac cgcggcggcg aagaagatcg ggtcctcgga
    18541 cacgtcgcga cccatcgtgg tcacccggag tccggcgctc cccagcaggt cggccgggtc
    18601 gggggtgtcg ctgacgacga gcggctgcac ccccgtcagc tcggacagct cctcgaccag
    18661 ctccgacggg gacgaggcga cccggggcga ggtgcggctc aaccgggcga ccgtctcggt
    18721 gtggtgcgac acgccccggt ggcgttccct gccgtcgccg gccgatgccc ggacgcacag
    18781 caccgtgtca ccccccaggg cggcggcggt gtcgatcacc gacgcggcct cgatcgcggt
    18841 ggtgccgagg gcggtcccgg tgcccaccac ccccggtccc atcccgacga cgaccacctc
    18901 ggcgccgagg gtgtggaccg ccaaccccaa cgccgaggcc accgtgaccg cctcgaggtc
    18961 gccgccgaag gcgtggcctg cggtgacggt gccgaccagc gtcccgtcgg cgcacagctg
    19021 tgccaccagg tccgacaggg cgagcggcag ggcggctccg tcggtcatga cgtacgcgac
    19081 ccgggttccg ggccgacgct cggccacggc gagcgcgacc agtggcacct ggctgtgaac
    19141 ggtgcaggcc acgaccggca ccccggcgat cgggcggtcg cactcgggat gcacgagctc
    19201 ggaggtgccg acgtcggcct gcagcgacgt gtagcgcatc ttcatgacgt ggtcggggcc
    19261 gggggccacc agctcgcggc gggccaggtt ccagtgcacc acgtgccagc cgccggttcc
    19321 caggccgagc tcgaccgcgg tcgtgttcag gaccacctcg tcaccgacgg cgcactcgcc
    19381 gaccagctcg accagggcgt aggcccgcgc cgggctcccg tcggccatgg cgaccgcgac
    19441 gcgctgcagg ccggggcgcg ccgagagcac ctcggtgacc gtcgcggtgc ggaacgtcgg
    19501 catcggtgcg tggggtcaga gcgattcgat gaggcgctcg acccgctcgt cgtgggcctt
    19561 gaacgggtcc ttgcacagca cggttcgctg ggcctggtcg ttgagcttca ggtgcaccca
    19621 gtcgacggtg tagtcgcgct tgcgctcctt ggcggtgcgg atgaactcgc cccgcagcct
    19681 ggcccgggtg gtctgcggcg gggtgtcgac cgcctcctcg atgtcggcgt cggtcacgat
    19741 gcgctcgacc cgacccgccc gctgcagctt gtagaacacc gagcggttcc ggctgatgtc
    19801 gtggtactgc aggtcgagca gcgccacgcg cggatgcgac agcggcaggt cgtggcgttc
    19861 ccggaacgcc tcgatcaggt tgtgcttggt cacccagtcg acctcgcggt ccaggctgag
    19921 ggggtcgcgc tccaggccgg tgacgacgtg ctcccacatg tcgagcgact gcttctcctc
    19981 gggggacagg tcgtggttgt cggcgtagcg cagcgcccgg ttcaggtact cggtctggat
    20041 ctcgagcgcc gaggcctcgc ggccgttggc caggcggacc cggcgggtgc aggtgatgtc
    20101 gtgggagatc tcgcggatgg cgcggatcgg gttctcgagc gtcatgtcgc gcagcaccac
    20161 cgtcgggtcc tcgagcatgc gcagcagcag tgcggtggcc cccaccttca ggtaggtggc
    20221 gtactcgctc atgttggagt cgccgacgat cacgtgcaga cgccggtacc ggtcggagtc
    20281 cgcgtggggt tcgtcgcggg tgttgatgat cggccgggac ctggtggtgg ccgagctgac
    20341 gccctcccag atgtgctcgg cgcgctgcgc catggagaac tgggcgccgc gggcggtctg
    20401 gaggaccttg ccggctccgg cgtagatctg gcggctcacc aggaacggga tcaggacctc
    20461 ggtgtagtgg gtgaggtcgt cgtggcggct ggtcaggtag ttctcgtggc agccgtagct
    20521 gttgccggcc gagtcggtgt tgttgcggaa caggtagatg tcgccgcgga tgccctcttc
    20581 ggccaggcgc tgctcggcgg actcgaggag ctgctcgagg atccgttcgc cggccttgtc
    20641 gtgggcgacc aggtcggcga gggagtcgca ctccggggtg gcgtactcgg ggtgcgaacc
    20701 gacgtcgagg tagagccggg cgccgttggc caggaagacg ttggaggagc gaccccacga
    20761 cacgacccgt cggaacaggt agcgggcgac ctcgtcgggg ctgagacgac gttggccgcg
    20821 cagggtgcag gtgacgccgt actcgttctc caggccgtag atccgtcggt ccatcgccgg
    20881 ctcaacctac cccgggacct ctgcggatcg gcgcgctccc ggaacggcgc gcgggttcag
    20941 gacggctcga tcagcggggt gagctcgtcg tcctcgatgc gctggaaggt ccggcggccg
    21001 ttgccccggg ccaggaccgc gacctcgagc tcggtgaccg ggatggtccg ctcgggtccc
    21061 gccagagcgg tcaccgcggc gttcaccgcc tgggcgaggg tcccgccctc gacccacgcc
    21121 gagcccatcc gctcgccgat ggcctcggcc tcgccgccga ggaccgccca tccggtctcg
    21181 tcgatcaggg tgccgtcgta gaggatgtgg aacagctggt cgccggcggg gtcgtgaccg
    21241 acctcggcga ccaggatctc gacctccatc ggcttcatct catgggtgaa gacctggccg
    21301 aggatctggg cgtactggtt ggccaggctg cgggcgtcga cgtcctctcg gctgaaggag
    21361 aagcccttca ggtccgcgtg gcggatgccg gcgatgcgca gctggtcgaa ctcgttgtac
    21421 ttgccgacgc cggcgaagcc gatccggtcg tagatctccg acaccttgcg cagcatgcgc
    21481 gacgggttct cggcgcacag gaggatgccg ctgtcgaagg tgcaggcgac gagcgcccgt
    21541 ccccgggcga tccccttccg ggcgtagtcc gcccggtcct tcatcatctg ctcgggggcg
    21601 acgtagaacg gcatggtcat gacgaggcac ctccggatga gcgctcgtcc tcgagctgtt
    21661 cgaacagcgt ctggtagcgg gtgcgcaggt cgtcgtcggc gacacgctcg aagccgtcgg
    21721 cgctgatggt ggcgaccacc gggaagatgc ccctcagcag gtcgggtccg ccggtggccg
    21781 agtcctcgtc ggcggcctcg tagagggccc gcaggacgag gtcgatgacc gcggtccgtt
    21841 cgaggccgtc gcggaagccg agcttgacga cggtggcggc gtggaggctg cccgatccgg
    21901 tcgcggcgtg gtcgtgctcc tcgtagcgac cgccggtcac gtcgtactgg aacagccgac
    21961 cccgcccggc gtgggtgtcg tagccggcga acagcggcac caccgccagg ccctgcatgg
    22021 cggcgggcag gtggttgcgg accatctgag agagctggtt ggccttgccc tcgagcgaga
    22081 tcgggctgcc ctcgaccttc tcgtagtgct cgagctgcag ctggaacagc cgcaccatct
    22141 cgaccgcggg tccggccgcg ccggcgatgg cgaccccgga gtgccgatcg gcggggaaga
    22201 ccttctcgat gctgcggtgg ctgatgaagt tgccggaggt ggcgcggcgg tcaccggcca
    22261 tcaccacgcc gtcggcgtag cgcacggcga cgcaggtggt cccgtgggtc accgtcaggc
    22321 ccgggccgtg tccgatgccg ggtgcgcctc ccagggtggc tccggttgca gcagcggtgc
    22381 ctgtggggtc gggcagcggg gggacgagcc cggcgcgacg cacgagctcg gcgaagttcg
    22441 gacccgggtc gtcctggggg ctgtagaggg gcaggttcac gggcggcagc gtacaagttg
    22501 gtcgccgcgg accgaggacc gccccggtcg gggcgagccg cggtccgtgc ggactactgg
    22561 ccgcccttct ggatgtaggc cttgacgaag tcgtcggcgt tggtctcgag cacgtcgtcg
    22621 atctcgtcga gcagctcgtc gagctcggcc ttgatcttct cgccggcctc ggtcgccgcc
    22681 ggagtggcct cgacctcgtc ggagcgacgc tggggggccg gcttcttctt gagttctcgt
    22741 tctgccatct gggggttcac ctccgcgtca gctgcacggt accgcggccg ccgtcggagt
    22801 cgtgtgcaca ttcccccgct ctgtcccgca ccagcgcggt gatcacgacg tctgtgcggg
    22861 acaaagccgg cgtgggcgtg ggcgcttgcg tgggggtggg ggcgggtcag cggccgaggc
    22921 ggtcgagcag gtccgcggcc gacgccgcgg tggcgagcag ctcgccgacg tgttcctcgg
    22981 ttccccgggt cggctccagc atcggcaccc gcctgagggc cggtgcgccg gtgtcgaaca
    23041 ccaacgagtc ccagttggcg gccacgatct gcgacgcgaa ccgtgccagg cagccgcccc
    23101 ggaagaacgc ccgggtcccc accggggcgt cggtcaccgc ctgctcgacc tcgtcgtcgt
    23161 cggtcaaccg ctccagaccc agccgtcggg ccagaccgcg cccagggcgc aggtcgtggt
    23221 actgcaggtc catggcggcc agtcgggggt cggaccactc caagccgtcc cgttcccggt
    23281 acgcccggta caggcggtac ttggccaccc agtcgagctg gtccgccaag ctcatcgggt
    23341 cgccgtccag acccgagagc accgactccc agcgggtgag gatctcttca ccgacagtct
    23401 cgggaccgag cacctccagt ccccgggact cggcgtaggc acgggcccgg ccgtacaggt
    23461 cccactgcag gtccagagcg gtgcggcggg acccgtcggc catctcgagc ggctcggcca
    23521 gcgacaggtc ccacgacacg gcctgcatcg ccgccaccgg ccgggccaac cgcaggggct
    23581 cgccgagggc gtcgtcctcg atcatggcga gcaccaacgc cgtggtcccg agcttgagga
    23641 aggtctggac ctcgctcagg ttggcgtcgc cggtgatcac gtgcagccgg cggtacttct
    23701 gggcgtccgc gtgcggttcg tcgcgggtgt tgatgatcgg ccgcttgagg gtggtctcga
    23761 ggccgacctc ctcctcgaag aagtcggcgc gctgggtcag ctggaacggc acctcgtcgg
    23821 tgcgcatgcc cggcgcctcg gatccgacct tgcccgctcc ggtgtagatc tggcgggtca
    23881 ccaggtgcgc ggtcgcgtgg gcgacgatgc gcccgaacgg gaccgcccgg tcgaccagga
    23941 agttctcgtg gcagccgtag ctgttgccct tgccgtcgga gttgttcttg tagacgacga
    24001 tttcctggcc ctcgggcagc gcggcgcgcg ccgcggccat cgagcggatc aggatctcct
    24061 caccggcgcg gtcgtgtacc agcgcggttc gggcgtcggg cgcctcgggg caggagtact
    24121 cggggtgggc gtggtccacg tagtagcggg ccccgttggt gagcaccgcg ttgaccaggt
    24181 gcgtctcgac ctcgggcgcc agcgacagca gcaaggcctc ctcgcgggcg tcgttgccgg
    24241 gcgactcgtc gtcgaagttc caaccgaccc gctgcgtgcg gtgcgcgcca cgctcaccct
    24301 cgagggcggc caggtacgag ttgatcagga tcgacgacgc cgagatcggg ttggactcct
    24361 cggccccccg cacgaggatc ccgtactcgg tctcgatccc gcagatcttg ggaatggcca
    24421 cggcccggca ccctagggca tcgggttgcg ggcccggtcg gtcacttcac gacgctgagg
    24481 gtgaccgacg ggtcgacccg caggtacacg atcgacgccg aacgtccgcc gttggtgctc
    24541 tcgagtccgg cggcgaagga cgcgtagatc gcccgctggg caccgtcgag gtcggtgaag
    24601 aagctgaggc cgcccgggcc ggtgcggccg ttggacgagg ccagccacgg cccggtcgga
    24661 tcgctggtgc acggtccggt cggggtcgcg cagcgggcga tcccggtgcg gtaggccgcc
    24721 tcgtaccagc gcccggccga gtacgacagc aggtagttgc gtcggacgcg gtcgtagacc
    24781 atcgaggggt tctcgatgaa gtggtactcc cacgggtact gacggccgag gaccgcggtg
    24841 ggccagccgg agatccaccc gttgccgtcg agggcgaaca cgtggatcgg cgtctcggtg
    24901 ccgccgaacg cgacgtacag ccagcgtcga ccctcggcgt cggtgaacac gtccgggtcg
    24961 agagcgccgt gatcgcccag gccgcaggtc atcgggacgg ggtcggcgac gaacggaccc
    25021 atcggcgagg tggcgatggc ccgaccgacg cactgggggt tggccggatc cggcgctccg
    25081 atgcggtcgg tgctgaagta catgacccag tggtcgccga accgggccac gctgggcgcc
    25141 cagaactggt tggggttggc cgcccatgcc ggggcggtgg gcatggcgtt ggtggtgacg
    25201 gcgttcttgt cccacaggtt gtacgggcgc gacaggtcgg tcagccgggt gacgggggca
    25261 cggacgtggc tgttggagcc gtacacgtag taggcgccgc cctcggtgag cacgaacggg
    25321 tcggaggtct ccggcaggcg gaccgcctcg acgccgacct tggtgacggg accgagcggc
    25381 acctggcagc cgctgaggag ggcgatcgca cccacgagag cgatcgtggc cgctccggcg
    25441 cggcggtgac gtcgacgcgg tcggtcggtg gcttccgggg tcgtggcgtc ccgagtggtg
    25501 gtgtcggtca tgagccactc ccctgctcgt ggtccgcggg cgcgacggcg gacgccgacg
    25561 cacgcgcgga tcctaccgat gcggctccgg cccggccccg gctcgtccac cacgcggcga
    25621 ggtagccgac gccgatggcc atgagcaggt cgaagggacc gcgcagccgc ggcaccgaca
    25681 ccgtcaccag cgacagcgcg acgaacccgg cgaccaccgc gagcgccggc cacgtccggc
    25741 ggtcccggat gcgcagcacg acccctgcgg tcccggcgag caccagcagc gggaacgtga
    25801 acagggtcgc gtcccggaac cgcgggtcgc ggccgtcgac gtactcggcc tcgaacgcct
    25861 ggaagcctgg gacctccaac atggtggccg cgccgctggc ggcgcgacgc agcaccgatg
    25921 cgggatgctc tcggatcccg tcgaccgcca gaccggtcag gtgatcgctc catgcggcct
    25981 cgtcgtcctg cagtgcccag acgccgtggt cgcggtacca actcgacacc gtcgggtcca
    26041 tgaagccgcc ggactcgatc gccgggggtc cgtacatggc ggcgagggtg aacccgtcgg
    26101 aggtgacgag cttgggggtt ccgacctcga ccgcgttgcg cagcgcccag ggggcgacca
    26161 ccgcgaccga gaccccgaac gccgccgcag cccggcgcca gcccgccgag cggacggcga
    26221 ccacgaccac gaccaggacg accaggtagg cgttcgggcg ggtgagcatc agcaggccgc
    26281 tgagcgctcc ggccagcacg aaccggtcct cgaggacgga gaggagcact cccatcatca
    26341 ggagcagccc gagcgactcg gtcatggtga cggtgtcgtt ggcgatcagg ctgggggtga
    26401 ccgccacgac cagcccggcg accagcccgg cgcggtcacc tgcgatccga cgggcgtagg
    26461 cgacggtcac gacgatcaga gcagcgccga tcccgaccgc gagcagtcgc cccggccaca
    26521 gggcatcggg tccgctcagc cgcatcggca gcgacaggag gaacgggtac agcggtgggc
    26581 ggaaggcggt ggggtgcagg gcctcaccgg ggaacgacgc gacgaagccc cggccggcgg
    26641 ccagggaccg ggccagatcc acgtactggc gggcgtcgga gcgcggcacc cagttcggcg
    26701 agatcacggc ccagtgcagg agtcggacga tgactccgac gacgaccggc acgacccacg
    26761 gcgggcgacg acgggtcggt tccgccgcag atcccggctc gccgtcgacc ggtggctcgg
    26821 cggcgaccgc ggggtggcgc accgcggtga cgtccatcgg tcgactctac ggaggaggcg
    26881 gcatgatccc acccctcgcg cgggcgagcc cggcggggcg gttcagaggt actggccggt
    26941 ggcgaccgct tcgatcgatc gaccgccggt cgggtcgtcg tccgcggcgt cgtgcaccag
    27001 ggtgcggacg tacacgatgc gctcgccctt cttgccggag atcttcgccc agtcgtcggg
    27061 gttggtggtg ttgggcaggt cctcgtgctc cttgtactcc tggtggatgg agtcgatgag
    27121 gtcctgcacc cgcacgccgc gggcgcccga gtcgagctga cgcttgatcg ccagcttctt
    27181 ggcgcggcgg acgacgttct cgatcatggc gcccgaggcg aagtccttga agaacatcac
    27241 ctccttgtcg ccgttctggt acgtgacctc gaggaactgg ttggcctcgt cggtgcggta
    27301 catctcctcg acggtgcgct cgatcatgat tcgcaccgcc ttgtcggtgt cgccgccacc
    27361 caacgtggtg atctcgtcgc tgtcgagcgg gacctcggcg gtgaggtagc gggcgaagat
    27421 ctgggacgcc gcggcctcgt cgggccggtt gatcttgatc ttcacgtcca gccggccggg
    27481 tcgcaggatg gccgggtcga tcaggtcctc gcggttggag gccccgatga cgatgacgtt
    27541 cttgagggtc tcgacgccgt cgatctcggc gagcagctgc gggacgatcg tcgcctccat
    27601 gtccgaggag atgccggtcc cccgggtgcg gaacagcgac tccatctcgt cgaagaacac
    27661 gatcaccggc gtgccgtcgg cggccttctc ccgtgcccgt tggaagacga gtcggatgtg
    27721 gcgctcggtc tcgccgacgt acttgttcag cagctcgggc cccttgatgt tgaggaagta
    27781 gctgcgggcc tccttgccgg tcgactcggt gaccttgcgg gcgagggagt tggcgaccgc
    27841 cttggcgatc aacgtcttgc cgcagcccgg ggggccgtac agcaggatcc ccttgggtgc
    27901 cggcaacgcg tactcgcgga acaggtcctg gtgcaggaac ggaagctcga ccgcgtcggt
    27961 gatcgcctcg atctgctcgt cgaggccgcc gatgtcggca tagccgatgt cggggacctc
    28021 ctcgaggacg agctcctcga cctcggggcg gggcagcttc tcgagcagca acgccgagcg
    28081 agggtccatc agcaccgtgt cgccggcacg gaggcgttca ccgagcaact ggtcggcgag
    28141 ctccgcgacg cgctcctcgt cggcccgacc gacgaccagt gcacggtggc cgtcgtcgat
    28201 catctccttg aacgtcacga tctcgcccgc gacctcgggc tcgcggacca tcaccacgtt
    28261 gtaggcgtcg ttgaggacca cctcgttgcc gcggcgcaga tcgtcgacgt cgatgccggg
    28321 cgacacctcg acgcgcatct tgcggccggc cgcttgcacg tcgacggttc cgtcgtcgtt
    28381 gatgcgaagc agggtcgcgt acgcggccgg cggctgggtg agcttgtcga cctcgtcgcg
    28441 cagcgcagcg atgtggtccc gggcctcgcg gagggtgaac gtgagcttct cgttctggct
    28501 cacggcctgg gcgagctggc ccttggtctc gagcagccgc tcctccagcg tccggacgcg
    28561 cttgggtgca tcctgcagcc gacggacgag gtgcgacacc tcgtcctcga ggtcccgcac
    28621 ctgctcgcgc agggcggcgg cgtcggtgcc gtcgaccggg ggccgcgggt cggtcgctcc
    28681 cccgtcgtcg acggtgtcct ccggagcggg ttcctggctg tcggggtcct gcacctggcc
    28741 tcccgggcgg ggcgtcgatg ggctcgtcct cggtcgttgt ctaccccgtc ccggccgcga
    28801 aaccgcgcca ccgaccggat cggatcccga cgtgacagga gtccggagag tttccaccgg
    28861 gattgccgtg taaatgccgg aggtgcgagt atgctctgac cacgaggagt cagcgccccc
    28921 gcccctcctt gaacgaacgt ggtggggccc gacgcgaagg caggatcaca tgggtatgaa
    28981 ggtgtggatc gaccaggacc tgtgcaccgg tgacggtttg tgcgaggaga tcgcacccaa
    29041 cgtgttcacc ctgctcgacg acggcctcgc ctacgtcaag gaaggcgaca agatcttcaa
    29101 cgacccgggc ggagccgagg gcatggccaa catccccgac gccgaactcg agggcgtgat
    29161 cgagtccgcc gaggagtgcc ccggcgagtg catcttcatc gaggtcgagt gacctccccg
    29221 gcctgaccgg ccggatcgga tcgaacgcga cgacggcggg atcctcagga tcccgccgtc
    29281 gtccgcgtcg ggcgtccggc gtcaggcgcc ggggtcctcg gaggtcggcg gcgcttcggt
    29341 ggtcgtcgtc gacgaggtgg tggtgctggt cgtcgtcggc ttctgcgtgg tcggggcagc
    29401 cgtcgtggtc ggccgaacgg tcgtggtcgg ccgggtggtg gtcggcgtcg acgtcgacgt
    29461 gctcgacgag gtcgtcgacg tcgtcgtggt ggagccggag tcgtcgccgc gcagcacggc
    29521 gaacgcaacc agagcgacca gcacgacgag cagggcgatg accgcccagg cccaacccgg
    29581 cacaccgcct ccggaccccg ggacgggagg cgtcgttccc ggtggtggcg ctgagccggc
    29641 gggaacgccg gtcggatcgg tgcccggcgc cggggcggcc ggggtgctcg gcatgacccg
    29701 ggtcgggtcc tcgggcgggt tctggtcgct catgtcatcc tccgggtctc ggtcccagcc
    29761 gggtgccgac gctaccgccg gtccccgtcg gcggcggtgg acctcggtgc cccctcgtca
    29821 ggacacgcac tccccggtgt cgaccgtcgc cccgcggggg cgggccagga gggcgtcgag
    29881 ctcggagcgg tcgagcgcgt aggcgacccc gtcgcggtcc ggggcgatgg ccaccgcgac
    29941 cgcgatgacc gtcccgtcgg accgcacgac ggcactgccc gagtcccccg gctgcaggtc
    30001 gctggccaac accagcaggt cgcggcgaac ctcccgacgg tcgtagatgt cgagcccggt
    30061 cgcatgcagc gtctcggcga cctcgaacgg cgacggcgcc agcggtccac ccccggggaa
    30121 ccccatcacc aggccgcggt cgccggtgcg gggtgcgtcg agggtgagtg cgggtcggtc
    30181 gagcgacgtg gccagcaacg ccaggtcggc ggccgggtcg aacgccacca cccgggcgtc
    30241 gcggcgatcg ccgtcagggg tgaccagctc gacgtcgtcg gtgccggcga ccacgtgggc
    30301 gttggtggcc acgagccccg gcgcgaccac gaagccgctg ccggactgga tcatgtcgca
    30361 ggcctcgccc cggacctgca ccacgctgtc ggtcagacgg gccagcgtcg cgtcgtcgat
    30421 cgggctgccg gtcggcaccg gcggcagatc cggcgtcggc tcctcgccgg tgaacagctg
    30481 ggggaagcgc ccgtcgagcg cctgacgctc cagctcgagc agtccggcgg gcgggtcggg
    30541 caggtgccgc aggctgaacg acgccagggt cgatccgtcg gtcgagcgct ccacccatcc
    30601 gggcgtctgc gacatgaccg ggaacaggag ccagcacacg acggcgaccc cgaccacgcc
    30661 gaggaccgct ccgccggcct ggtcgatccg ccgggccgcg ccgggccgca ccggcggcgc
    30721 gatccgcgcc ccgatcgcgg acccgatccc ctgccccagc gatgccagca ccaccaggac
    30781 cccgagcccg acgaacaccg ccgcgctgct cgatccgagg tcgagtcgat cgagcagtgg
    30841 aggcagcagc accaccgcca gccccaaccc catcccggcg ccgatccagc cgaccgaccg
    30901 ggtcaggaac ccgagcttcc accccccgac cgcggcgagc acggcggcga ggacgatcac
    30961 gacgtcgagg ccgttcaccc ggtgcccctc acccagaacc cctcacccgg ccccccatct
    31021 ggtcccgcag ccgtcgcgag gtcgactcag gtgcccagtc gggcgacggt caggaacccg
    31081 gtgtgggcga ccatgcgatg gtcgggtcgg accgcctggc cctcgacgta ccagccccgc
    31141 tgcaggacct cggtcgtggc cgcggcgaag aagcgcgact cctcgagggc ggtccgcagc
    31201 tgcatcacct gggtcacgct cggggtgtag gccaggatca gcccgccggg gcgcagcgcc
    31261 ttctcggcgt gggggacgac ctgccacggc tcggggaggt cgagcaccac ccggtccgcg
    31321 tcgcgctcgt cgatgccctc gtagcagtcg cgcagctcca cccggtagcg ctcgaggacc
    31381 tcgggtccga ggaaccgttc gacgttctgg accgcccgct gggcgaagtc ctcccgcagc
    31441 tcgtagccga cgatgtcggc gccgctgcgc agcatcccca tggacagggc gcccgacccg
    31501 acgccgctct ccagcacccg catgcccgga ccgatgtcgg ccagcatgag gatcgggccg
    31561 atgtccttgg ggtagatgac ctgggcgccc cgcggcatct tgaggatgaa ctcgctgagc
    31621 gtgggtcgaa tcacgagcag cggctggccc cggttcgtct cgacgcgcga gccctcggcg
    31681 acgccgatca cgtcgtcgtg gcggacgtag ccggagtgga ggtggaactc cttgcccggg
    31741 gtgagtgtgg cctggtagcg ccggtccttg gggtcgacga actggaccag gtcgccggcg
    31801 cgcagcagtg ggtcggtcac ggtgaggggg ttcctgtggg gtctggtccc cctcggcggc
    31861 cgagggtgac gggtcgggcc ggcggggcgt ccggcgggga tccgccggcc ctctccacca
    31921 gtcggcagaa ggcgcacacc tcggtcgagg tgggcgaccc gcagcgggcg cagggtgcca
    31981 gcaccccggg gtcagcccgg tcggcgtcgc ccgtgccggg ttcgggagcg aagcggtcca
    32041 cggcgcggcg caggaacgag aagtagaagt cggccttgga gccgggcgag cgcacctcga
    32101 tctcgttgag cgcctccttg tagccgaggt gcttgttccc cgccgacatc gggcactcgt
    32161 cgaccaggta gtcgatcccg ctcacgatgc agtaggccgc ggtctcgcgt tcggagagcc
    32221 ggaccagcgg cttgaccttg cgggggaacc cgtcgcgggc gggcagcacc gggcgttgcc
    32281 gacccaggta gtcggtctgc caacgcagca cgttgccgaa cagcacggcg gcctcgtcgt
    32341 cgaggttgtg gccggtgacc aggacgtcgt agccgccgtc gagcgcggcg cggtcgaaca
    32401 ggtgtcgctt ggacaggccg cacgcgctgc acggcgtccg ccgcgccgcc cgggcgcccc
    32461 cggggatgtc gtagccgtag tcgtcgacca ggtcgatctc gatcagcttg gcgccccggg
    32521 gttccgcgtg ctgctgcgcg aatcggcggg cgtatccgcc cgaggtgtcg ctgtagtcgc
    32581 cgatgccgag gccgatgtag aggccgtcgg cggcgtagcc gagcccgagc aggaggtccc
    32641 agaccgccag ggagtccttt cctcccgaca ccgcgaccag gatccggtcc tcgggttgca
    32701 tcatgtcgaa gtccgagatg gcccgctcga cctggtcccg gcagaaccgg gtgaagtgct
    32761 cggggcagaa gttggcgttg tggcgcggca ggtcgatcac cgccggcccg cggcacacgc
    32821 ggcacttcac gcgctgcccc cggagatgac cggacgcacc tcgacgacgt cgtcgggctc
    32881 gaggcgctcg tcaccgggaa cgatcgtgcc gttgcgcacg accagcaccg actcgcggtt
    32941 gatgtccagg cgctcgagca gcaccgagac cagcaccgga gcgtcggcgt ggacctctcg
    33001 gttggggttg cgcagccgga ccagcatggt ccccatcatg ggcgatgggg ccgtcgtcgt
    33061 cccaggcgga tcgctatggt cggccgccgt gaccctctgg aacgacccgc agatcgagag
    33121 ctggcaggcc cggggccgtc gtgtgacggt gtccgggccg gcgctgccga cccaggacgt
    33181 cttcgtcgtc gacgtggccg cggcagatcc cgaccccgcc ctcgaggtgc tgctggtggt
    33241 ccacggcttc cccacctcga gcttcgactg ggcgcacctg gtcgacgaca tggcgagaca
    33301 ccggcgggtg gtcctggtcg acctgcccgg cttcgggctg tcggacaagc ccgatcggca
    33361 ctacacgatc gccggtgccg cggacgccgt ggccggcgtg accagcgccc tcgccctgga
    33421 ccgggtggcg cttttgaccc acgacatggg cgacacggtc ggcggagagc tgctggcccg
    33481 tcagatggag ggcacctggc cggtggagat cacccggcgg gtggtgacca acggctcgat
    33541 ctacatcgac atggcgcagc tcaccgacgg ccagctgatg ctgctgtcgc tgcccgacga
    33601 gcgcgggccc gccggtccgg gtcccgagct gctggccgcc agcctgcagg ccaccctggc
    33661 cgaggcccac cgcgccggga ccgacatgac cgcggccgct gagctcgtgt gccacgacgg
    33721 cggcgacgcg ctgctgccga ggaccatccg ctacatcgag gagcggcggt ccaacgaacg
    33781 gcgcttcacc ggggccatcg agacgcaccc ctcgccgctg cacgtcctgt ggggtcccga
    33841 ggacccgatc gcggtgccgt ccatgaccga ccgactgctc gaggcccgac ccgacgccac
    33901 ccgcacctgg atcgacggtg ccggccacta cccgcagaac gaggacccgg ccgcctacct
    33961 ggcggccctg gagcaggcgt tgggctgacc gccccgttcg acgacggggt cggcccggtc
    34021 gggttccggc ccggcgac
//
LOCUS       DAOQOD010000011        32918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_428_length_32918_cov_14.928156, whole genome shotgun sequence.
ACCESSION   DAOQOD010000011 DAOQOD010000000
VERSION     DAOQOD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 32918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32918
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_32918_cov_14.928156"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLZ72_02475"
     CDS             complement(<1..242)
                     /locus_tag="PLZ72_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA35193.1"
                     /translation="MSTGFDQLHSVVQHHVVNSLGWRELRPLQDQAVAPILAGEHVLA
                     LAPTAGGKTEAAAFPVLSQILTERRPGLSTLYVCPL"
     gene            complement(261..602)
                     /locus_tag="PLZ72_02480"
     CDS             complement(261..602)
                     /locus_tag="PLZ72_02480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNA35194.1"
                     /translation="MPSSGDVVELDLGTPEGREAGFRHPAVVVTAQRILDAEPSVIHV
                     VPLTSTIRGFHSEVVIQVDPTNGLDVVSAAQCQHIRAVSTHRIADVRGNVGAALLVQL
                     REAIAVILDLP"
     gene            complement(586..780)
                     /locus_tag="PLZ72_02485"
     CDS             complement(586..780)
                     /locus_tag="PLZ72_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35195.1"
                     /translation="MHSTSVRIDTTTHEELKRLAAELHTTVGNTVTLAVRALRQERVG
                     ADLRTPLRPDETDWLDAELG"
     gene            complement(1011..1487)
                     /locus_tag="PLZ72_02490"
     CDS             complement(1011..1487)
                     /locus_tag="PLZ72_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35196.1"
                     /translation="MLALLEERLPADLVVTDPADDRIVALDRAPIEFLAGNQWMFANA
                     YWDSLAAFVQPNSPAIDQVLEATRQLPKDRSGNASTEGFVGSVRSRSDRVRDHLGNMF
                     GEMTATSSGAAATARDGQASESRQTRQRSTSPIWKGPMPLQLRTASTSIPVSTQES"
     gene            complement(1556..1960)
                     /locus_tag="PLZ72_02495"
     CDS             complement(1556..1960)
                     /locus_tag="PLZ72_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA35197.1"
                     /translation="MATEASESTADLDLRVREVLATLAREMSGKRVICVTHEDTMRAF
                     RFRLEKMTIEQWNRQGSSNRLDIVNCGILHYTRRDSSGTVRERFERVRTIDPSRPDEA
                     RWRRIRRPRFSNEDLLAQVGRHPHLWDDDEVG"
     gene            complement(1864..2391)
                     /locus_tag="PLZ72_02500"
     CDS             complement(1864..2391)
                     /locus_tag="PLZ72_02500"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNA35198.1"
                     /translation="MGEEITTGMPIDLLLVRHGHSEGNRALESAEDGDVRLHQDRRYR
                     ARSAADYRLTAEGRAQAATAGVWLRSWLDDEGLEGFDRWYCSPFVRTRETAALLGIDT
                     AQWQLESLLRERDFGLWEGLSKDVAAEMHARSNQMKKRYKFLWRPRRARAPPTWTYGS
                     GRSWRLWPARCRARG"
     gene            complement(2475..3863)
                     /locus_tag="PLZ72_02505"
     CDS             complement(2475..3863)
                     /locus_tag="PLZ72_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA35199.1"
                     /translation="MHDVEPLSPEDATLLCATPPEAQLQIGALCRFEGGPLRNTDGGL
                     RLDDLRDHVAACIHLIPRFRQRVRHVPFGLARPVWIDDVDFDLSHHVHDAVLPAPGGP
                     AELRRFVADLLGRPLDPDHPLWDLWLIDGLDDGNVAVVLRAHHVVADGLSLLRAATAL
                     LDFDADPSPDPPPDPWTAASEPCPAALAIGALAHRRRRQLELLVDAGRAVLDPRRSLS
                     LATSVLTGVTAPPTAPRTALTGRVGTRRDFLWASVSLESMHRLARSRGVTINDVVLAA
                     VTGALRRNMGDEPGPSMNRAPKVLVPIGDAADGHGGNRFSFVVAGLPVHLEDPGAVLD
                     HVHREMSTRKTSGQARRMLRLFSVVDLLPVPLLRATAPGVLARQPLVNLAVTDLPGSE
                     ASLYLLGSRLLAMHPIVSGVGNIACIVGVLSYGDRLGIGLTVDPDVVRDPDRFMADLV
                     GASNELCGLAAP"
     gene            complement(3969..4775)
                     /locus_tag="PLZ72_02510"
     CDS             complement(3969..4775)
                     /locus_tag="PLZ72_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA35200.1"
                     /translation="MHHDHSDPGLVDVDGSPAVTAGPHRLSMVYGGGGVFGIGYCVGV
                     AHGLAAGGIPVVGAPSLGTSAGSWAASAVATGVSYDDVAGLEVPSIPSRRGGVLADVA
                     RGIFGEATHPLVSVSAVCLRTRRRHILDGGRYPLADLVAASSAVPGLLPPHRIDGRLY
                     MDGGMWSVTSIDAAADAEEVIVVAPLAGPHMGPIGRTAGVLLDRELRTWRRRHPDRII
                     TLIRPNREIASHLGRNPMSLFDGDRARDIYPLSYEQGLRWAERLRSAQAA"
     gene            4991..5632
                     /locus_tag="PLZ72_02515"
     CDS             4991..5632
                     /locus_tag="PLZ72_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35201.1"
                     /translation="MGVDCARAWVASVEHRQRDVASPLVRCVDAGLADGSAFFATYAA
                     WTAVRLGVVGRLPGAVRDLPSRVEGVLVGAWADGVVAGLDGDAAGLDAVGEQFVELGA
                     SIDAVEAFAAASRLWSTDGRARAATASIRRARESLGDSDVRTPGLVLSDDAVPLTDRE
                     REVADLAADGLAAREIAERLFISRRTVTNHLQHVYDKLGVSGRSELRTALGRG"
     gene            6228..7016
                     /locus_tag="PLZ72_02520"
     CDS             6228..7016
                     /locus_tag="PLZ72_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35202.1"
                     /translation="MALIAGIVMAPSAGAARIGGSLPPIFSAHLYGQGTSVVASYRTV
                     ATVRTMAVVATAPLPAGHDLYGTAGMNDRVRGIATTPAATTHLTVTEGLLAGTEYFVQ
                     LYTEGPGGSWVLQQRNRSVRTQDRYVRARFDKIKIRNDGDPMGCGELQFQLKVAGAWG
                     DEPYFMTNENSMCTGETYNMNIVSPWVKVNADTTTAYSLGQDDDYYGGVLCTYGFGGC
                     GDSAQKTVTLYLSTMKHLESRPMSVWANGGFEFQWLGSYEVEYR"
     gene            7304..8215
                     /locus_tag="PLZ72_02525"
     CDS             7304..8215
                     /locus_tag="PLZ72_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35203.1"
                     /translation="MSRPKKRKGGRVTPKGTGSTSRPPGGLRPGGPNPLGRGPMGPLD
                     GPWLDQFDDDGDVDGEVGDGDTDVLARAGDAITTGHPAELLVLASTFAEAVADAARTD
                     PLIPGSTDDELYSWRTVVDELTGSTRPETTALLHALRPFVPDEWNQVITAELSERHTP
                     SLPAWISSIGEAEVTSVWCRSHDLGHDDDVFIGARWPTGQVLTLVVHVDHELGGSVQD
                     VILMPADIDQVLRVFAAPADGPTSLTEVDLADARAQIAAAVDRWEQSSPRTLTDDWPT
                     ARPLLLWLLSRLPLGGPDASASGDSRT"
     gene            complement(8218..8619)
                     /locus_tag="PLZ72_02530"
     CDS             complement(8218..8619)
                     /locus_tag="PLZ72_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HNA35204.1"
                     /translation="MNLHRCSFAPAVPVEVDGAAFAEPWNSLAAPWTNPWNGEVVEIL
                     DDRHVGRIPTGPQLHAAAFGTGGSMTDGPGGGDADAGAAQGSPAGAAALALTGSSTST
                     AVAIGFGAISSGVAALHLRRRAVERATSASS"
     gene            8995..10842
                     /locus_tag="PLZ72_02535"
     CDS             8995..10842
                     /locus_tag="PLZ72_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35205.1"
                     /translation="MSRPKKRRGGRVTPKQTRPRNWDDPGPSSHRGGPAPDELPLLAE
                     IREALSAGHPLGLLALGSTFAELATDAERPRSMLSDEPGSERPQVTWEMIVDMLSATA
                     LPETTALLHALVLFAPPPWDSHLRVELEQRRLHQLPGWVRTLDEVEVTQVWEQVDVLG
                     DGENILLGLRWSTGDELTVVLYIDHNIGGLIKDFISGLGDAEEILTMFEAEAEAGSSQ
                     RRIDPADARARIESAVQRWSMTWPPVLSDEWPVARPLVQWVASKLPEGGVMPTREELS
                     DEDRDRLIDSFLGSPDGDAHRDDPDVQTLLIATMWFGADHGVCDPLKWSPVRVELLLT
                     DFIPRKLLVDAEMLAKLPELLVDFLPWALRALGDRDGIDRQVIDERIDDALEALDHWA
                     PQYLMLIDADPDEWDGDVFGDGSAYEMARAILAEAGDDFDPEARERLESFVALGPDPS
                     ERDYLLYELGGEEAAAAVHDDPWPLEPVDLARVPDDVQEKVTEIDEQLVAVCTRLSLE
                     PEHITIARRLLVRVAERDPQALRRRGAIGGWVAGLIVVVGDMNRLIGQRHGQVTKKAI
                     AEAAGVKSVGDKDITIGRAAQLMSAYHHVDLLDSRARQHLLELLAGYED"
     gene            complement(10889..12340)
                     /locus_tag="PLZ72_02540"
     CDS             complement(10889..12340)
                     /locus_tag="PLZ72_02540"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNA35206.1"
                     /translation="MGKQNRARRAAKARARQSARRDADARQARSSRAAGPTGGPEPFA
                     TDDRSRRSEPQRATDALLSAAHAQVGSSRRIRASEDLLSMSAPVVDRAAEELALTGVS
                     AAWGGGWQPNELARHLRLRGCSAATGRLGRTAIAADHAGRSSSTLDSRWAAQVQAMEL
                     PDGVRARGWIRRWSDEERIDRSATLDTVVDLLGILVALRRIEQLLPPPGSAADPSRSG
                     RTDGTGGPTGTSPSEGTELDPLLEKIRNLLAKAESTTFEAEATALTAKAQELMTRHAV
                     DAAALHAGDDTDGELAPVAIRIPIDAPYTDAKSLLLQTVALASRCRSVHDPDLDLSTV
                     VGFPDDVAVVEVLFTSLLVQAQTALTDAARHAPPGTRTRSQSYRSAFLLSYTHRIGDR
                     LEEINQHVIDQATIDHGGSFLPVLRSRSDRVEDHMRAMFTTMTSKPVRGGYDRAGWAS
                     GQLAADRAAINLADLEQADPTTTGSPRPVKELT"
     gene            12453..13916
                     /locus_tag="PLZ72_02545"
     CDS             12453..13916
                     /locus_tag="PLZ72_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HNA35207.1"
                     /translation="MDHDQIDHLRKHPAWSLLRANNAALVLSFLTHTYIDRSAGNISS
                     PELVSRLDDHLFAVNQQLGEERYPRSAKDYLEEWADAEHGWLRRFYPPGSDEPHFDLT
                     PAAEKAAAWVDGLGDRTFIGTESRLTMLIELLRQLVVESDDDPAVRLADLRRRRDALD
                     EEIERIETGGVAAADPVVQRDRYQQFARSARELLSDFREVEANFRTLDRDLRQKIAGW
                     SGSKGELLDDVVATRSGIAESDQGRSFRAFYELLLTAERQDELTDLLDRLHRLEDLPD
                     LDQRLARVHHDWIDASERTQRTVRQLSEQLRRFLDDQVWLENRRVFDLLRSIEANAVT
                     LREHGSVPVTTTVDALRPTVVLPMERPLYRRTRTEALDGGPLDGPDDQVLDTSSMFDQ
                     LHVDRQALVGAVHRTLGAGRSVSLDDVLVDSPLEHGLAELVAYFSLHESQLALDFDDT
                     ERSQVAWVDDDEHVERRADVPRVTFSRPEPAGQGSEP"
     gene            13913..14617
                     /locus_tag="PLZ72_02550"
     CDS             13913..14617
                     /locus_tag="PLZ72_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HNA35208.1"
                     /translation="MNDELSAPPMTSVDASTSGTATVAAGPSELSLLVITLFKGPMYR
                     DVHDRLWPILLKLRARVTDHVAVMGLRLEVDENEGYAFLRGLRDDETDVEYPRLVNRH
                     TLGFHTSLLIALLRKRLAEFDATSSDTRLVLGRDQIVEMLRVYLPSSSDDVKTMRVVE
                     GHIRKVEELGFLHPLRGQDDQYEVRRIIRAYVDGQWLGDFDRRLRDYVESVAGGGADA
                     VDDSAADVVRGDGREG"
     gene            14598..18110
                     /locus_tag="PLZ72_02555"
     CDS             14598..18110
                     /locus_tag="PLZ72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35209.1"
                     /translation="MTDGRAEVGGADRDPLPGTRLRRLEVFNWGTFDRHVWTLEVAGR
                     NALLTGDIGSGKSTLVDAVTTLLLPANRVAYNKAAGADTRERDLRSYVLGYFKSERNE
                     ETGGTRPVALRDHRQYSVILGVFGRGDGGPSVTVAQVFRGREDGTQPERFYVVADGDL
                     SITSDFDASTGATLGDLRKALKVRGAQVEESFPPFAKALRRRLGIPSDQALDLFHQTV
                     SMKAVDDLNGFVRSHMLEPFDVDRRVEDLIEHFENLTAAHDAVLRARDQLRLLEPLAE
                     TLDAHAVVMAQLADLTAIDRALPTWFDERLRSLLLAEVSAADVEIDRLTSAIERSTES
                     AVGLDQQRTELSIQIAGAGGDRLAAIDAETAQLDAALPTRRHDREAFDALVAQVGLTP
                     VTDVDGFASMQAGLAERRDELDALAAGSDAEASDLEQERRALDAEATQINEELRSLRG
                     RQGNLPRRSTEIRAELCRALGVEAELLPFVGELLQVRPGAADWEGTAERVLRSFAMSL
                     LVPEEHYGSVATWVNAQHLGAKLVYHRVPDRLGRRADTGRSGTAPLLLDMVEVKPESK
                     FADWLTNELWHRADHECVDTIEAFRRSPKAVTRHGQVKSRDRHEKDDRYRVDDRRNYV
                     LGWDNELKVAALIDHARELHARGAGLTERIGECAERRRMLSDRLGDVRVLAARERWED
                     LDPDALTARRDELAAEATRIRESSDVMRALTDQLEAVERERHAVGVELADLQGRLGAQ
                     RDRSDRARRRLDALDVAVPAAITVPPPLPTGAVVAGSLFDAEPEPVPAAGPVGVWLPV
                     DIAERANDLATIDAARHDAAGLLAEQTATAHDRRSKLDNRAAAQMREFGHAYPREVEE
                     LDARIESAAEYRAIHDRVATDDLPRFEADFRRQLKENAIHEIAGLSAELHRHEEEIRR
                     RVEVINASLEAIDYNPGRYIRLVPAATPNTEIRQFREDLRACTSGMTRGTDDDQYSEE
                     RFLQVKALIDRLRGREGTTDQDRAWRRRVTDVRQWFVFSASERWREGDVEHENYSDSS
                     GKSGGQKEKLAYTILAASLAYQFGLGDGGSGPAGDDGSFRFVVIDEAFGRGSDDSTRY
                     ALKLFTELGLQLLIVTPLQKLHVIEPHVACVGFVDNVNGDRSRLQRITVEELQLRRSE
                     HRAPLAEPAAGGAPAAGSPDGLGR"
     gene            18107..19402
                     /locus_tag="PLZ72_02560"
     CDS             18107..19402
                     /locus_tag="PLZ72_02560"
                     /inference="COORDINATES: protein motif:HMM:NF023223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HNA35210.1"
                     /translation="MSPRASRSSSADGWSRPEDMVAKLRDRWDRGTYLRARADASPWE
                     PVRVRLVGPTATDLLERPGESVAWIDGVRAWAAGGRAGPRCRIEDRTVRSRSLGDNDV
                     PGFVVVDSIEELAMLVGRPGQIATFDRLLALTAERRPELVSWVSAHPMDVLEVVADWL
                     SMLDVVDWIDRTDTSTVHLRQLDLPGVDTKFVERHRRLLGRLLQQVLPPERVDTSASR
                     FDARFGFRTVPSYVRFRILSPVEELPAGLSELSVRVDELARLALPVRTVFVVENQVTY
                     LAFPDVPDAIVVFGEGFKASTLDAVPWLADRELVYWGDIDTHGFRILDQLRARVPGVR
                     SLLMDESTLSAHLDRVVSEDAPTSVPLEHLSSEESDLYRDLVEDRFGPSVRLEQERVR
                     FGAVRGALRPWAGGGPAGSPADDRPGFGRVGGSSDHVPA"
     gene            19577..20764
                     /locus_tag="PLZ72_02565"
     CDS             19577..20764
                     /locus_tag="PLZ72_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA35211.1"
                     /translation="MTAVRPGRSVTVLVANPSSDIYGSDLQMLESITAMVERGWKVVV
                     VTPDDGPLVALIEERGAEVLRVDCAVVRREYASPSGLWRLVRESFSSLRNLLRVLRTL
                     RPTLVYTNTITIPWWFVAARLCRIPVVCHVHEAEAEDPLPVRLVLYGPLLLAHRLIVI
                     SRPSMETVCATVPRLRRRATMIFNGVREPTEPPRPAVAGPGPRRLVVLGRLSPRKGTD
                     VALEAVALLRRSGRDVTLDVCGSTFPGYEWFEEQLRRRAAESDLDGAVDFSGYISPIW
                     PAIERAEVSVAPSLREPFGNAVVEAQLAQRPVVAAAAMGHLETVIDGETGLLVTPGDA
                     QATADAIARLLDDGDLAARIAETARRTAEERFSLTRYRREVADLLAAVMKLPAGGSEG
                     RSA"
     gene            complement(20761..21633)
                     /locus_tag="PLZ72_02570"
     CDS             complement(20761..21633)
                     /locus_tag="PLZ72_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35212.1"
                     /translation="MDKLLFGDNQFFGVNHMSEEKARAQAMRFQDIDAVMAVLDAAYD
                     EGIHTFMCTTHDRIAEVCDRVRAQPDRYSEFTFFPCMPYAHKYANAVTESGMLGAVRR
                     FLPEEGFINAAIRGGRSMATKDIEGMATLLIDAEMAMFRGLRTPVIWMQNIVVDLLLG
                     LGVDEAFRIFADHVRDRYDAEPGFITMNLPALLDVLERQGIDNPIVCANINKIGFRMS
                     GGMDAYKDALQNRRCRVVAMSVFASGAIPPREALEWIYEQPNIHSVVFGASSRANIRN
                     TRELVDEFTRAGQG"
     gene            complement(21633..22817)
                     /locus_tag="PLZ72_02575"
     CDS             complement(21633..22817)
                     /locus_tag="PLZ72_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA35213.1"
                     /translation="MIRIAVVGLGKMGLSHLAMLRAHPEVELAAVVDSTPYMVDVLSK
                     YSGLRTFTDMSRALQEVELDAVVIATPTRLHVQMVREALENGLHVFCEKPLCLTPEDS
                     ESLAELAASRGLVTQVGYHNRFVGAFREVRNLLDMGAIGRVTHALAEAYGPVVLKPKG
                     GTWRSQRSEGGGCLYDYAAHPVDLLSWYLGAPSSVGGTVLGQVFSAETEDEVYSTLHY
                     PDGSSAQLSVDWSDESYRKMTTRVTLWGTEGRIFADRQECQVYLRDTATCPPGYEHGW
                     NVRYTTELTDEVWFYLRGEEYSAQLDYFVHRVAKEQPDGLNDFASAAVTDRVLDTMTR
                     DAASSRVTTTSGSGPAPAPTSSRTSTGQLTAELLRRALEGVRRLATRAKDALVSARRS
                     RK"
     gene            complement(22814..23989)
                     /locus_tag="PLZ72_02580"
     CDS             complement(22814..23989)
                     /locus_tag="PLZ72_02580"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HNA35214.1"
                     /translation="MTATLRATQPTVRILGTHGVPANYGGFETAAENVALFLRDQGWR
                     VVVYCQDEGRGRIRTDVWNGIERVIVPVPLDGWLGTSLFDLRSILHAVRHRDLCLTFG
                     YNTAIFNTLQRLLRIPNVINMDGIEWSRARWGRARQSILWTNERIACVVGDALIADHP
                     EIAVHLATRAPERKITTIAYGAHPVTEASTAPVTALGLEPGAYLTLICRPIPENTILE
                     LVRAFSRRERGVRLVVLGTYRPDEDEYHRAVMDAASDEVDFVGAIYDPDSVTALRFHG
                     IGYLHGHTVGGTNPSLVEAMAAGNAIVAHDNDYNRWVAADGALYFRTEDDADAAITSL
                     LEDRALRDRLSAAARDRHLAEFTWTHVAGQYEDLLVRVGGVRKPVNDSATCTTGGHR"
     gene            complement(24175..25092)
                     /locus_tag="PLZ72_02585"
     CDS             complement(24175..25092)
                     /locus_tag="PLZ72_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNA35215.1"
                     /translation="MSVPPSGQQFEIRHGDQRATIVEVGGGIREYRVGARHVLDPYPL
                     DEMCTGAHGTPLIPWPNRLADGRYRFDGRDHQTALTEPAKHNAIHGFLRWRSWEGIDR
                     DEHRVVMATTLHPMPGYPFTLHVEVAYELTSDGLAVTTTAENRGDWPCPYGCGSHPYL
                     SPGDGLIDGCTLQLDAATRITTDAVRQLPTGTEPVEGTAFDFRSPRAIGDLQVDFAFT
                     DLTRDDDGRAWTRLTGTDGAEAALWVDRHHPYIEIYTGDTLPPDKCRRGLGTEPMTCP
                     PNAFATGEDVIRLEPGASSTTSWGVRLNT"
     gene            complement(25095..25592)
                     /locus_tag="PLZ72_02590"
     CDS             complement(25095..25592)
                     /locus_tag="PLZ72_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA35216.1"
                     /translation="MIRHDLSKILGQWRPGAIWVKREEDHELVYEAPDRELVPQLIGE
                     LASTLDTISDTDDHAVLRAAMAHLNLVMIHPFSDGNGRMARCLQTVERLTSDQSSPGL
                     EHAERLGQFGEHLQVRPHEFDAVAQRRVLDAGLRQAEFEGAGTTRPAAHRRVAASLVS
                     HAPGG"
     gene            complement(25592..25897)
                     /locus_tag="PLZ72_02595"
     CDS             complement(25592..25897)
                     /locus_tag="PLZ72_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35217.1"
                     /translation="MSDADEAVIARCLRRSIDGAYFAGDEFHTLLGLHRHEMETIVDA
                     WPDPPTEAPEGYANGHEAQHVAVNNVLNSLLGYPHGRSPATRIRRPSTSPCSSPCTS"
     gene            complement(26041..27231)
                     /locus_tag="PLZ72_02600"
     CDS             complement(26041..27231)
                     /locus_tag="PLZ72_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA35218.1"
                     /translation="MRPDAFTSTERGRVHKAPEGYWAFHPTEAPRRLSLSDQVIKLLD
                     EATGAVHRLGGVGRLIPNPHLLIGPHLRLEAVLSSRIEGTKTDVSELLRFEAGQVLPG
                     EAADDATEVRNYIVAMEHGLARVREGFPISIRLFREMHERLLDGARGQHRRPGELRRS
                     PVWLGGTTLDDAVFVPPPPDEMNHALSDLERFLHATDLPLLVQLAVAHYQFEVIHPFL
                     DGNGRIGRLMIPLMLVLREALPQPLLYLSAFLEQHRSEYYDHLLLTSQKGDLMPWIAF
                     FLRGVRQQSRDSEERTVRLVELQHQLRNELLDEGRPNSVVRLAEQLFAVPLVTAPRVE
                     AAIGVTRPTAQAAIDALVERGDLTEITGRSRGRVYEASAIYEAVYGPVSFAASTVHPE
                     LPFE"
     gene            27464..27718
                     /locus_tag="PLZ72_02605"
     CDS             27464..27718
                     /locus_tag="PLZ72_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35219.1"
                     /translation="MQFEAPEGDEYVITGVERVSQTWVVHYQSRRHLESGEIRHARAG
                     NGPIRVTGDYTTTVADTALPTEHFVAEFESRGGETLDGHP"
     gene            complement(27915..29396)
                     /locus_tag="PLZ72_02610"
     CDS             complement(27915..29396)
                     /locus_tag="PLZ72_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35220.1"
                     /translation="MSVWRPQPEDLQRVFDEQNPWQRDGRVPDALARPVERTLAKLLW
                     RRLRDDEPHRFQLVLGPRRVGKTTALYQTVRHLISDGTDPKRIWWLRLDHPLLLQEQL
                     GDLVRAVIESSGATDASPVFLMLDELVYTDRWDQWLKTFYDDRWPVRIAATSSATAAL
                     RDRRLESGVGRWAEQHLTPYLFTEFLELVDAGKDAPVDRHLSESIRALPHGTRSDPDV
                     TRWRRQFMLIGGFPELLTLPRDRPNDDGTRLIESQQILRSDAVERAVYKDIPQSFGVD
                     SPMMLERLLYVLAAQITGILSPSNICKDLGLSQPTFDRYLSYLEQAFLVFTLPNYSGR
                     EATVQKRGRKLYFVDGAIRNAALQRGVAPLDDPAEQGALLENLVAATLRSLAVHAGVR
                     LHHWRDGKHEVDLIFDHPEHPMAFEIASSPDHSRSGMRALVDRHPRFARNTYLVAPQV
                     AVAHPSATSDFGTLPLDLFLLVVGAQAEAAFVAGLGGGKPDTD"
     gene            complement(29681..29890)
                     /locus_tag="PLZ72_02615"
     CDS             complement(29681..29890)
                     /locus_tag="PLZ72_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNA35221.1"
                     /translation="MTKNMTVRLPDELAADAEALARVEGKSVNETIKDALSAAIDQRR
                     RDPKFRQRVRQIIEEDRVLLERLAK"
     gene            complement(29956..30351)
                     /locus_tag="PLZ72_02620"
     CDS             complement(29956..30351)
                     /locus_tag="PLZ72_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35222.1"
                     /translation="MLFLTTGVRRGELAGLRWTDVELDRATMTIVRNRVSAWRGKTVS
                     TRPHLASYHPEVELLVEEAPSLTVTAVWLWIRCAPGRAAIRPNSLSSTGLSTPRRSVS
                     RTGFLAQRAMNIADRYESAAPVNVGSAPG"
     gene            complement(30558..30863)
                     /locus_tag="PLZ72_02625"
     CDS             complement(30558..30863)
                     /locus_tag="PLZ72_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35223.1"
                     /translation="MARTTIDDLKARRLGQMSEAELAEFDATYAATKLALEVGEQVRD
                     AREAAGLTQRELASRMGTSQAAIARLEAGGVGATLTTLQKVATALNLEVAVLLRPAS"
     gene            complement(30863..31204)
                     /locus_tag="PLZ72_02630"
     CDS             complement(30863..31204)
                     /locus_tag="PLZ72_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA35224.1"
                     /translation="MWSVELEPEVEEWLDTLSTIEFATVLPHIERLADRGSALRMPAS
                     RPLGDGLFELRFDMSNAAWRIPFFFVPERRIVLLTVFRKQRMNERQEITRARAAMTRC
                     IAEAHTAEEDD"
     gene            complement(31320..31700)
                     /locus_tag="PLZ72_02635"
     CDS             complement(31320..31700)
                     /locus_tag="PLZ72_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35225.1"
                     /translation="MQPSTLCRAFVIAVISTGTLGASGCAIGWQDQPISRVLVSADVR
                     SLDVGWHCHKEADVEVEESEDEVRLRFRVDSYKGGCADLERVTLASALGDRRIVDSTT
                     GKAVTPCLDTSEGAVGTMTAPECQ"
     gene            complement(31796..>32918)
                     /locus_tag="PLZ72_02640"
     CDS             complement(31796..>32918)
                     /locus_tag="PLZ72_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA35226.1"
                     /translation="PGTWELRVDAGEDPLTRKRQQKSVTFHGTSRQADVALAELITKS
                     ARGQVSVGQRTVAHAIEAGLRQAELEGLEPTTLRSYRTSAECHVLPALGSRRLSHLSA
                     EHLDRFYGALVEAGYSRSTVRGCHVLLCRVLDQAKRWGWVAVNVGRDARPPRQHTSNP
                     KPVPIDVARAMIDEAVRVNPTLAMLSVLAADTGARRGELCALRWRHVNVDAGTLRIEA
                     AIGETNVVYEKDTKTHQHRTVTLSSFALDWLLDHRDRHAKACALCGIELSNDACVLAP
                     EPGGLKPLHPSSATRAFSRLRGRMELPSWIHLHGLRHLQVTQLLDAGIPLRSVSGRVG
                     HRNPSTTTNIYAHWIQESDAHSAKVVGGRIWGSAERGQP"
BASE COUNT         4903 a        11135 c        11712 g         5168 t
ORIGIN      
        1 ctcagcgggc acacgtacag cgtcgacagc ccagggcggc gctcggtcag gatctgcgag
       61 agcaccggga acgccgcggc ctcggtcttg ccgccggcgg tgggagccaa cgcgaggacg
      121 tgttctccgg cgaggatcgg ggcgacggcc tggtcctgca acggtcgcag ctcgcgccag
      181 cccaggctgt tgacgacgtg gtgctgcacg acggagtgca gttgatcgaa cccggtgctc
      241 atggggcgat ccgagatgcc tcagggcagg tcgaggatga ccgcgatcgc ctcgcgcagt
      301 tgcaccagca gggctgcgcc gacgttgccg cggacgtcgg cgatccgatg ggtcgagacc
      361 gcccggatgt gctggcactg cgccgcggag acgacgtcga gtccgttggt cgggtcgact
      421 tggatgacca cctcggagtg gaagccccgg atcgtgctgg tgagcggcac gacatggatc
      481 accgacggct cggcgtcgag gatgcgttgg gcggtgacca cgaccgcggg gtggcggaag
      541 ccggcttccc ggccttcggg cgtcccgaga tccagctcga cgacgtcacc cgagctcggc
      601 atcgagccag tcggtctcgt ccggccgcag cggtgttcgc aggtcggcac cgacgcgctc
      661 ctggcgcagg gcacgaaccg cgagcgtcac cgtgttgccg acggtggtgt gcagttcggc
      721 ggcgaggcgc ttgagctcct cgtgggtggt ggtgtcgatc cgcacgctcg tgctgtgcat
      781 gcagatcagc atacggcgcc ctcatcccac cggcgatggt tgctgcggtg gcggtgaaga
      841 ggaggagtcg gtcaccggcg ccggcgcctt agaaaccttg cagaagacct gccgtggcga
      901 tgatcccgtc gttggcagcg aagacccaat cgcgccgtgt ccttggatcg cgcacccatt
      961 cccggacgtc gttccagcgg tggcgatcga tcgacgtttg caccgtcagg tcagctctcc
     1021 tgcgtgctca ctggaatcga tgtgctggct gtgcgcagct ggagtggcat cgggcccttc
     1081 cagatcggcg aggttgatcg ctgcctggtc tgccgcgatt cggacgcctg gccatcccgc
     1141 gcggtcgcag ccgctccgga gctggtggct gtcatctcgc cgaacatgtt gcccaggtgg
     1201 tcccggacgc ggtccgagcg cgaccggaca cttccgacga acccctcggt gctggcgttg
     1261 ccggatcgat ccttcggcag ctggcgggtg gcctcgagca cctggtcgat cgccgggctg
     1321 ttgggttgga cgaatgccgc cagcgagtcc cagtaggcat tggcgaacat ccattggttt
     1381 ccggccagga actcgatcgg tgctcggtcc aatgcgacga ttcgatcgtc ggccgggtcc
     1441 gtgacgacca ggtcggcggg aaggcgctcc tccagcaggg cgagcatccg ccgattgggt
     1501 gtgaggcgca cccgacggat cgggaccgcc aggcccgggc gccccgtcgt cgggcctaac
     1561 cgacttcgtc gtcgtcccag agatgtgggt ggcgcccgac ctgcgccaac aggtcctcgt
     1621 tggagaaccg cggccggcgg atccggcgcc acctggcctc gtcgggtcgg gagggatcga
     1681 tggtccggac ccgctcgaag cgctccctca cggttcccga gctgtcccgg cgcgtgtagt
     1741 ggaggatgcc gcagttgacg atgtcgagcc ggttggagct cccctgccgg ttccactgct
     1801 cgatcgtcat cttctcgagc cggaaccgga acgcccgcat ggtgtcctcg tgcgtcacgc
     1861 agatcaccct cttgcccgac atctcgcggg ccagagtcgc caggacctcc cggacccgta
     1921 ggtccaggtc ggcggtgctc tcgctcgcct cggtcgccag aggaacttgt agcgcttctt
     1981 catctggttg gagcgggcgt gcatctcggc ggcgacgtcc ttgctcaggc cctcccacag
     2041 cccgaagtcg cgctcgcgga gcagcgactc gagctgccac tgggccgtgt cgatgccgag
     2101 cagcgcagcg gtctcgcgtg tgcggacgaa cggtgagcag taccaacggt cgaacccctc
     2161 gagtccctcg tcgtcgagcc atgaccggag ccacacgccg gcggtcgctg cctgcgcacg
     2221 gccctcggcg gtcaggcggt agtccgccgc gctcctcgcc cgatagcgcc gatcctgatg
     2281 caagcggacg tcaccgtcct cggccgactc gagcgcccgg ttcccctcgc tgtgcccgtg
     2341 gcggacaagg agcaggtcga tcggcatgcc cgtggtgatc tcttctccca tgcggagcac
     2401 tctgccgcga acggggtgcc gtgacggagg gctgcgtccg tcggtgtcgc tccattcgat
     2461 cgtcgggaac cggctcaggg cgcggcgagc ccgcacagct cgttcgaggc tccgaccagg
     2521 tcggccatga agcggtccgg gtcccgcacc acgtccgggt cgaccgtgag gccgatgccc
     2581 agacggtccc cgtaggacag gacgccgacg atgcaggcga tgttgccgac acccgagacg
     2641 atggggtgca tcgcaagcag ccgggacccg aggaggtaca gggacgcctc ggaccccggc
     2701 agatcggtga cggccaggtt gaccaggggc tggcgggcga gcaccccggg ggcggtggcc
     2761 cgaaggagcg gcacgggcag gaggtcgacc acggagaaca gccgcagcat ccgacgagcc
     2821 tgacccgagg tcttccgggt cgacatctct cgatggacgt gatcgaggac cgcacccggg
     2881 tcctcgaggt ggacgggcag gccggccacg acgaacgaga accggttgcc cccgtgcccg
     2941 tcggcggcgt ctccgatggg gaccagcacc ttcggagcac gattcatcga gggtcccggt
     3001 tcgtcgccca tgttgcggcg cagggcaccg gtgacggccg cgagcaccac gtcgttgatg
     3061 gtgacgcctc tggagcgggc caacctgtgc atcgactcca gcgacaccga ggcccagagg
     3121 aagtcccgcc gggtgccgac ccggcccgtc aacgcggttc gcggcgcggt cggcggagcc
     3181 gtgacgccgg tgaggaccga ggtggcgagg gagagagacc gtcgggggtc gagcacggcg
     3241 cggccggcat cgacgagcaa ctccaactgt cgacgtcgac ggtgggcgag cgcgccgatc
     3301 gcgagagcgg ccgggcaggg ctcggacgcc gcggtccagg ggtccggcgg agggtccggc
     3361 gacgggtccg cgtcgaagtc gaggagcgcg gtcgctgctc tcagcagcga cagcccgtcg
     3421 gcgacgacgt ggtgggcgcg cagcaccacg gcgacgttgc cgtcgtcgag gccgtcgatg
     3481 agccagaggt cccacagggg gtggtccgga tcgaggggtc gacccagcag atcggcgacg
     3541 aaccgtcgca gctccgcagg gcctcccggt gcggggagca cggcgtcgtg gacgtggtgc
     3601 gacaggtcga agtcgacgtc gtcgatccag accggccgag cgaggccgaa cggcacgtgc
     3661 cgcacccgtt ggcggaaccg cgggatcaga tggatgcagg cggcgacgtg gtcgcggaga
     3721 tcgtcgagcc gcaggccacc gtcggtgttg cgcagcggtc ccccttcgaa acggcacaga
     3781 gcgccgatct ggagctgggc ctccggtggg gtggcgcaca ggagggtcgc gtcctccggg
     3841 ctcagtggct ctacatcgtg cacccgatca cctctctcgt gtccgtgtcg tcatgcgacg
     3901 ggcgggtccg ccaccgtccg ccacggtccg aggccctccg actcaccgac gatcgacccc
     3961 gggcctcctc aggcggcctg tgcggatcgg agtcgttcgg cccagcgcag gccctgctcg
     4021 tagctcagcg gatagatgtc cctggcccgg tcaccgtcga agaggctcat cggattccgc
     4081 ccgaggtgcg acgcgatctc gcggttcgga cggatgagcg tgatgatccg gtcggggtgc
     4141 cggcggcgcc aggtgcgcag ctcccggtcg agcagcactc ccgctgttcg accgatcggg
     4201 cccatgtgcg gcccggccag gggcgcgacg acgatcacct cttcggcgtc ggccgcagcg
     4261 tcgatcgacg tgaccgacca cattcctccg tccatgtaca ggcggccgtc gatgcggtgc
     4321 gggggcagca gccccggcac cgcggacgag gcggcgacca ggtcggcaag ggggtagcga
     4381 ccgccgtcga ggatgtggcg gcgtcgggtg cgcaggcaca ccgccgagac cgacaccagt
     4441 gggtgggtcg cctcgccgaa gatcccgcgg gcgacgtcgg ccaggacgcc accccgtcgg
     4501 ctgggtatcg acggaacctc gagcccggcg acgtcatcgt aggagacgcc cgttgcgacc
     4561 gccgaggccg cccaggatcc ggctgaggtg ccgagtgagg gggcgccgac gaccgggatg
     4621 ccgccggccg cgagcccatg ggcgactccg acgcagtacc cgatgccgaa cacgccgccg
     4681 ccgccataga ccatcgacag ccgatggggc ccggcggtga cggcgggtga gccgtcgacg
     4741 tcgacgagtc caggatcgct gtggtcgtgg tgcatgacga cctccgctgc ggagagcgat
     4801 ccgacgtcga accccgccga caccattctc gaccagcagg acacccgggc gatcccggat
     4861 ccggcgtgac gtcgggcgca gcgcgtccgc tgctgagacg gatccgcgga tgcagctcgc
     4921 agagcgatcc cgccgggccg gcgcccaccg gagcggccga gtggtcgacc tactcggatc
     4981 agccccggtc atgggtgtcg actgcgcccg ggcctgggtg gcatcggtcg agcatcgcca
     5041 acgggacgtg gcgtcgccgt tggttcgatg cgtcgacgcc ggtctggccg acggatcggc
     5101 gttcttcgcc acctacgcgg cgtggaccgc ggtgcggctc ggcgtggtcg gccgtctccc
     5161 cggcgcggtg cgagatctgc cgtcgcgggt cgagggcgtc ctggtcgggg cgtgggccga
     5221 cggcgtggtc gccggtctcg acggagacgc ggcggggctc gacgcggtgg gtgagcagtt
     5281 cgtcgagctc ggcgcctcga tcgacgcggt cgaggccttc gcggcggcgt cgcggctctg
     5341 gtccacagac ggtcgggctc gtgccgcgac cgcatccatc cgccgagccc gggagtcgtt
     5401 gggcgactcc gatgtccgga ccccgggact cgtcctgtcc gacgacgccg taccgctgac
     5461 cgaccgggag cgtgaggtgg ccgacctggc ggccgacggg ttggccgcca gggagatcgc
     5521 agagcggctc ttcatatccc ggcggacggt gacgaaccac ctgcagcacg tctacgacaa
     5581 gctcggcgtg tcgggacgat cggagctgcg gaccgcgctc ggcaggggct gatccggcgc
     5641 ggttcggccc gtcctccgcg atcgatgagt agtcggtgct caggtccgca tcctgggccg
     5701 atgctgcgat ggatccatgt cggacgcaga cccgtcggct ctgtcgccgc tcgctggacg
     5761 tctcgctgcc gccctgtgcg acgacgccga tctgctcgcc gtggcgctcg atcgtcctga
     5821 ggacgtcgtc ggtgcgtggg ggctcagttc gactgatgtc gtcgctgctg gccgctgcct
     5881 ggacgccgtc gcccggtgtc gccagggacg ggcggccagc cgagtggcgt tgttccgtct
     5941 cgtcctggcc tgccggtccg tcgaggccgg acccggtgtg accagatgag catcgatcgc
     6001 tcagggcgcg tcgaagcgga tctgtcttga tggtcgcggt cagccagacc aacgacgaaa
     6061 cacacgaagg agctccacat ggaccatgag acgcatcgcc gtcctctccg accaactgcc
     6121 gccctgaccg gctcgcgaca tctgagcccg agagcggtcg ccccgggcag cggctcccgc
     6181 accagaggtc gcgctcgccg gctcaccctg cgctcggtca ccgtgtggtg gcgctcatcg
     6241 ccgggatcgt gatggccccc agcgccggtg cggccaggat cggtggaagc ctccctccga
     6301 tcttctccgc ccacctgtac ggccagggga cctcggtggt ggcgtcgtac cggacggtcg
     6361 ccaccgttcg taccatggcc gtggtggcga cggcgccgct gccggccggt cacgatctgt
     6421 acggcacggc cgggatgaat gaccgggtgc gcggcatcgc cacgactccg gccgcaacca
     6481 cgcatctgac cgtcaccgag ggcctcctgg caggaaccga gtacttcgtg cagctctaca
     6541 ccgagggtcc gggtggctcg tgggtgcttc agcagcgcaa ccggtcggtg cggacccagg
     6601 atcgctacgt gcgggcccgg ttcgacaaga tcaagatccg caacgacggc gacccgatgg
     6661 gttgcggcga gctgcagttc cagctgaagg tcgccggcgc ctggggcgat gagccgtact
     6721 tcatgaccaa tgagaacagc atgtgcaccg gcgagaccta caacatgaac atcgtctccc
     6781 cgtgggtgaa ggtgaatgcc gacaccacta ccgcctactc gctgggccag gacgacgact
     6841 actacggcgg ggtcctgtgc acctacggct tcggcggttg cggcgacagc gcccagaaga
     6901 ccgtcacgct gtacctgtcg acgatgaagc acctcgagtc ccgacccatg tcggtgtggg
     6961 ccaacggcgg cttcgagttc cagtggctcg gctcctacga ggtcgagtac cgctgagctg
     7021 acgcagggtc ctccgccggt cgatccggtg gcgccgcatg gggcttcgtg gcccgcgcgg
     7081 cgccaccgtg tccggtgcgc ccgtcgaccg accgatggaa ggcggtcgag cgcaacgcca
     7141 gactcgaacg ggtcgctcgg agtacctctc gagtcggggg cgaaggtcgt tgaccagggc
     7201 gggcagcagg cacacatggc gtgtcgacgg tccggtccgg cggctgggtc gggatccgcc
     7261 ggacgcgccg atgtgtgcat cggactcccg tagcgtcggt cccgtgagcc gaccgaagaa
     7321 gcgcaaaggc ggcagggtca cccccaaggg caccggatcc acgtcgcgtc cgccaggtgg
     7381 actccggccc ggtggaccca acccgctcgg ccgagggccg atgggtccgc tcgacggacc
     7441 gtggctcgac cagttcgacg acgacggtga cgtcgatggt gaggtcggtg acggtgacac
     7501 cgatgtgctg gcacgggccg gtgacgccat caccaccggc caccccgcgg agctgttggt
     7561 gctggcgtcg accttcgcag aggccgtggc cgatgccgca cggaccgatc cgctcatccc
     7621 ggggtcgacc gacgacgagc tgtactcctg gcggacggtc gtcgatgagc tcaccggatc
     7681 gactcgcccg gagacgacgg cgttgctcca cgccctccgg cccttcgtgc ccgacgagtg
     7741 gaaccaggtg atcaccgcgg agctgtccga gcggcacacc ccgtcgttgc cggcatggat
     7801 ctcgtcgatc ggcgaagccg aggtcaccag cgtgtggtgc cgcagccacg acctcggtca
     7861 cgacgacgac gtgttcatcg gtgcccggtg gcccaccgga caggtgctga cgctcgtcgt
     7921 gcacgtcgac cacgaactgg gcggatcggt gcaggacgtg atcctgatgc cggcggacat
     7981 cgatcaggtg ttgcgcgtgt tcgccgctcc ggcggacggg ccgaccagcc tgaccgaggt
     8041 cgacctggcc gacgcccggg cgcagatcgc agccgcggtc gatcgttggg agcagtcatc
     8101 accccggacc ctcaccgatg actggccgac cgctcgcccg ttgttgctgt ggctgctgtc
     8161 ccgacttccc ctcggtggtc ccgacgcgtc ggcgtccggt gacagtcgga cgtgaggtca
     8221 actggaggct gatgtcgccc gttcgaccgc tcgtcgtcgg aggtgcagag ccgcgacacc
     8281 ggagctgatc gcaccgaacc cgatggcgac agccgtcgat gtcgacgaac cggtcagggc
     8341 cagggctgct gcgcccgccg gtgatccttg agccgcccct gcatcagcgt caccgccccc
     8401 cggtccgtcg gtcatcgacc cgccggtacc gaatgccgcg gcgtgcagct gcgggcccgt
     8461 gggtatccga cccacatggc ggtcgtcgag gatctcgacg acctcgccgt tccaagggtt
     8521 ggtccaggga gcggcgagcg agttccacgg ctcggcgaat gcggcgccgt cgacctcgac
     8581 cggcaccgca ggcgcgaacg agcatcggtg caggttcatc gccacagcgt cggcctcggt
     8641 gcgggcgtag cggtgagcgg cggcgttccg cgaggcccgg gagcgcagcc gagtcttccc
     8701 gtgcggtccc cgcgggtagc cccagtcggg atcgaggcgc cacaccgtca ccgtcgtcgc
     8761 ggccgcgacg ggcgtggcac cgacgacggc gaccgcggct gcggccccgg ccgcgccgga
     8821 gaccgggcga ccgccgtgca ggtgccgagc gggtgtcggc cccgaccgct ccggcggtcg
     8881 gcccggtgtc gatgtagccg ggtgcggctc cctcgggggg gttgcgtcga ctcggggccg
     8941 cacatcgaag tggacgatcg cggtggggcc tccagcgcgc cctaccctcc gggcatgtct
     9001 cgtcccaaga agcgccgtgg tgggcgcgtc accccgaagc agacgcgtcc ccgcaactgg
     9061 gacgatccgg gcccgtcgtc gcatcggggc ggacccgccc cggacgagct cccgctcctc
     9121 gccgagatcc gagaggctct gtccgcgggc cacccgttgg ggctgctggc cctcggctcc
     9181 accttcgccg agctggcgac cgacgccgag cgcccacggt cgatgctgtc cgacgagccg
     9241 ggttcggagc ggccgcaggt cacctgggag atgatcgtcg acatgctgtc ggcgacggct
     9301 ctgcccgaga cgacggccct cctgcacgcg ctcgtgctgt tcgccccgcc gccgtgggac
     9361 tcccatctcc gcgtggagct ggaacagcgt cgactccacc agctgcccgg atgggtgcgc
     9421 acgctcgacg aggtcgaggt cacccaggtc tgggagcagg tcgacgtgct cggcgacggc
     9481 gagaacatcc tcctgggact ccgctggtcg accggtgacg agctgacggt ggtcctctac
     9541 atcgaccaca acatcggggg tctgatcaag gacttcatct ccggactcgg cgatgccgag
     9601 gagatcctga ccatgttcga ggccgaggcc gaagcaggat cgtcgcagcg tcggatcgac
     9661 ccggccgacg cccgtgcccg catcgaatcc gcggtgcagc ggtggtcgat gacgtggccg
     9721 ccggtgctca gcgacgagtg gccggtcgcc cggccgctgg tgcagtgggt cgcgtcgaag
     9781 ctgcccgagg gtggggtgat gccgacgcgc gaggagttga gcgacgagga ccgggaccgg
     9841 ctcatcgact cgttcctcgg gtcgcccgac ggcgacgcgc accgcgacga ccccgatgtc
     9901 cagacgctcc tcattgccac catgtggttc ggcgcggacc acggcgtgtg cgacccgctc
     9961 aagtggagcc ctgtgcgcgt cgagctcctg ctcaccgact tcattccccg caagctcctc
    10021 gtcgacgccg agatgctggc caagcttccc gagctgttgg tcgacttcct gccgtgggcc
    10081 ttgcgggcgc tcggtgaccg tgacggcatc gaccggcagg tgatcgacga gcggatcgac
    10141 gacgctctcg aggcgctcga tcactgggcg ccgcagtacc tgatgctcat cgacgcggat
    10201 ccggacgagt gggacgggga cgtgttcggc gacggctccg cgtacgagat ggcccgggcc
    10261 atcctcgccg aggcgggaga cgacttcgat cccgaggccc gcgagcggct ggagtcgttc
    10321 gtcgcgctcg gtcccgaccc gagcgagcgc gactacctgc tctacgagct cggcggcgag
    10381 gaggcggcag ccgcggtgca cgacgacccg tggccgctcg aaccggtcga cctggcaagg
    10441 gttcccgacg acgtgcagga gaaggtcacc gagatcgacg agcagctcgt cgctgtctgc
    10501 actcggctct cactcgagcc ggagcacatc acgatcgccc gtcgactgct cgtgcgggtc
    10561 gccgagcgcg acccgcaggc tctgcgtcgc cgtggagcga tcggcggctg ggtcgccggg
    10621 ctgatcgtgg tcgtcgggga catgaaccgc ctgatcggcc agcgccacgg ccaggtcacc
    10681 aagaaggcga tcgccgaggc cgcgggtgtg aagtcggtcg gcgacaagga catcacgatc
    10741 gggcgggcgg cacagctgat gtcggcgtac caccacgtcg atctgctcga ctcccgggcg
    10801 cgtcagcacc tgttggagct gctcgccggg tacgaggact gatccgaggg ctgatccagc
    10861 gggtgcggga ccctcgacgg ggggaccgtc aggtcagctc cttgacgggc cgcggcgaac
    10921 cggtcgtggt ggggtcagcc tgctccaggt cggccaggtt gatcgcggcg cggtccgcgg
    10981 cgagttggcc gctggcccat cccgcacggt cgtagccgcc ccggaccggc ttggaggtca
    11041 tcgtggtgaa catggcgcgc atgtggtcct cgacgcggtc cgaacgcgat cgcagcacgg
    11101 gcaggaagga cccgccgtgg tcgatggtgg cctggtcgat gacgtgctgg ttgatctcct
    11161 cgagccggtc gccgattcgg tgcgtgtagg acagcaggaa cgctgaccgg taggactggc
    11221 tccgcgtccg ggtgcccggc ggtgcgtggc gcgctgcgtc ggtgagtgcg gtctgggcct
    11281 gcaccagcag cgaggtgaag agcacctcca cgaccgcgac gtcgtcgggg aacccgacca
    11341 ccgtcgacag gtcgaggtcg gggtcgtgca ccgatcggca ccggctcgcc agcgcgaccg
    11401 tctgcagcag gagcgatttg gcgtcggtgt acggggcatc gatcgggatg cggatggcga
    11461 cgggcgccaa ctcgccgtcg gtgtcgtcgc cggcgtgcag cgcggccgcg tccacggcat
    11521 ggcgggtcat cagctcctgg gccttggcgg tgagcgccgt cgcctcggct tcgaatgtgg
    11581 tcgactcggc cttggccagg aggttgcgga tcttctccag cagcgggtcc agttcggtgc
    11641 cttccgacgg cgacgttccg gttgggccgc cggttccatc ggtgcgaccc gagcggctcg
    11701 ggtcggcggc cgaacccggt ggaggtagca gctgttcgat gcggcgcagc gcgaccagga
    11761 tcccgaggag gtcgacgacg gtgtcgaggg tggcgctccg atcgatgcgc tcctcgtcgg
    11821 accagcgtcg gatccagcct cttgctcgga ccccgtcggg gagctccatg gcctggacct
    11881 gggctgccca tcgcgaatcg agagtggacg agctgcgccc ggcgtgatca gcggcgatcg
    11941 ccgtccgacc caggcggccc gtggcggcgg aacaaccccg gagtcggagg tgtcgggcca
    12001 gctcgttcgg ctgccaaccg ccgccccacg ccgcggagac accggtcagg gccagctcct
    12061 cggcggcgcg gtcgacgacc ggcgccgaca tcgacagcag atcctccgac gcccggatgc
    12121 ggcggctgct tccgacctgg gcatgcgcgg cggagagcag cgcgtctgtg gcccgttgcg
    12181 gttcggatcg tcgggatcgg tcgtcggtcg cgaacggctc cgggccgccg gtcggccccg
    12241 ccgctcggga gctcctcgcc tgtcgggcat cagcgtcacg cctggcggac tgccgtgctc
    12301 gggccttggc ggcccgacgg gcgcggttct gctttcccat gggtccgacc gtagccgtgg
    12361 cgtgtgacag ggccccgggc ccgggccggc ccgcctcgtc tgtcatcgca ggcccctacc
    12421 ctgtcggtcg accgctcgga cccggaccca tcatggacca cgaccagatc gatcacctcc
    12481 gcaagcaccc ggcctggtcg ttgctgcgcg ccaacaacgc ggccttggtc ctgagcttcc
    12541 tcacccacac ctacatcgac cgcagcgccg gcaacatctc ctcgcccgag ctggtgtccc
    12601 gtctcgacga ccacctcttc gcggtcaacc agcagctcgg tgaggagcgc taccccagat
    12661 cggccaagga ctacctggag gagtgggccg atgccgagca tggctggctc cgccgcttct
    12721 acccaccggg ttccgacgaa ccgcacttcg acctgacccc ggcggcggag aaggcggcgg
    12781 cctgggtcga cggcctgggc gaccgcacct tcatcggcac cgaatcccgt ctcaccatgt
    12841 tgatcgagct gctgcgccag ctggtggtgg agtccgacga cgacccggcg gtccgcctgg
    12901 ctgacctgcg gcgccgacgt gacgcgctcg acgaggagat cgaacgcatc gagaccggcg
    12961 gggtcgccgc cgcggacccg gtcgtccagc gcgaccgcta ccagcagttc gcccgctccg
    13021 cccgcgagct gctgtcggac ttccgcgagg tcgaagccaa cttccgcacc ctcgaccgtg
    13081 acctgcgcca gaagatcgcc ggttggtccg gttccaaggg cgagctgctc gacgacgtcg
    13141 tcgccacccg cagcggcatc gccgagtccg accagggccg cagcttccgc gccttctacg
    13201 agctgctcct caccgccgaa cgtcaggacg agctgaccga cctgctggac cgcctccacc
    13261 gactggagga cctgcccgac ctggaccaac gactggcccg cgtgcaccac gactggatcg
    13321 acgccagcga acgcacgcag cggaccgtcc gtcagctctc cgaacagctc cgacgcttcc
    13381 tcgacgacca ggtctggttg gagaaccggc gggtgttcga cctgctccgc tcgatcgaag
    13441 ccaacgccgt gacgctgcgc gaacacggct ccgtgccggt gacgaccact gtcgatgcgt
    13501 tgcggccgac cgtcgtgctg cccatggagc ggcccctgta ccgccgcacc cgcaccgaag
    13561 ccctcgacgg aggtcctctc gacggccccg acgaccaggt gctcgacacc tcctcgatgt
    13621 tcgaccagct ccacgtcgac cgccaggccc tcgtcggcgc ggtgcaccgg accctcggcg
    13681 ccggtcgatc ggtgtcgctc gacgacgtgc tggtcgactc gccgctggag cacggcctgg
    13741 ccgagctcgt cgcctacttc tccctgcacg agtcccagct cgctctcgac ttcgacgaca
    13801 ccgaacgctc gcaggtcgcc tgggtcgatg acgacgagca cgtcgaacgc cgggcggatg
    13861 tgccccgggt gaccttctcc cgaccggagc ccgccggaca ggggagcgag ccgtgaacga
    13921 cgagctgtcg gctcccccga tgacgtcggt cgacgcgtcg acgtccggaa ccgcgaccgt
    13981 cgccgcgggg ccgtcggagc tctcgttgct cgtcatcacc ctgttcaagg ggccgatgta
    14041 ccgagacgtg cacgaccggc tgtggccgat cctgctgaag ctgcgcgccc gcgtcaccga
    14101 ccacgtcgcg gtcatggggc tgcgcctcga ggtcgacgag aacgagggct acgcgttcct
    14161 gcgcggcctc cgcgacgacg agaccgacgt cgagtacccg cgtctggtca acagacacac
    14221 gctcgggttc cacaccagcc tgctcatcgc gctgctgcgc aagcgcctcg ccgagttcga
    14281 cgccaccagc agcgacacgc gcctggtgct cggacgcgac cagatcgtcg agatgctgcg
    14341 ggtgtacctg ccgtccagct ccgacgacgt gaagaccatg cgcgtcgtcg agggccacat
    14401 ccgcaaggtc gaggagctcg gcttcctgca cccgctgcga ggccaggacg accagtacga
    14461 ggtgcgacgc atcatccggg cctatgtcga cggccagtgg ctcggcgact tcgaccggcg
    14521 actgcgcgac tacgtcgagt ccgtcgccgg cggtggagca gatgccgtcg acgacagcgc
    14581 cgcggacgtg gtccgtggtg acggacggga gggctgaggt gggcggcgcc gaccgcgacc
    14641 cgctgcccgg cacccgtctg cgtcggctcg aggtgttcaa ctggggcacc ttcgaccgcc
    14701 acgtgtggac cctcgaggtg gccgggcgca acgccctgct caccggcgac atcggctcgg
    14761 gcaagtccac gctcgtcgac gccgtcacga cgctgctcct gccggccaac cgggtcgcct
    14821 acaacaaggc ggccggtgcc gacacccggg agcgggacct gcgctcctac gtgctcggct
    14881 acttcaagtc ggagcgcaac gaggagaccg gcggcacgcg gccggtcgcg ctgcgggacc
    14941 accgccagta ctcggtgatc ctcggggtgt tcggccgggg cgacggcgga ccgtcggtca
    15001 ccgtcgccca ggtgttccgc ggccgagagg acggcaccca acccgagcgg ttctacgtcg
    15061 tcgccgacgg cgacctgtcg atcacgtcgg acttcgacgc gtcgacggga gcgacgctgg
    15121 gtgacctgcg caaggcgctc aaggtccgtg gcgcgcaggt cgaggagtcg ttcccgccct
    15181 tcgccaaggc gttgcgacga cggttgggga tcccgtcgga ccaggcgctc gacctgttcc
    15241 accagaccgt gtccatgaag gccgtcgacg acctcaacgg cttcgtccga tcccacatgc
    15301 tggagccctt cgacgtcgac cggcgcgtcg aggacctcat cgagcacttc gagaacctga
    15361 ctgcagcgca tgacgccgtg ctgcgcgccc gtgaccagtt gcgcctgttg gagcccctgg
    15421 ccgagaccct cgacgcgcac gccgtcgtca tggcccagct cgcggacctg accgcgatcg
    15481 accgggccct cccgacgtgg ttcgacgaac ggctgcgatc cctgctgctc gccgaggtct
    15541 cggccgctga tgtcgagatc gaccggttga cgtccgcgat cgaacgcagc accgagtcgg
    15601 ctgtcggact cgaccagcag cgcaccgagc tgtcgatcca gatcgccgga gccggcggcg
    15661 atcgcctcgc ggcgatcgac gccgagaccg cgcagctcga cgcggcgctg cccacccgac
    15721 gacacgaccg cgaggccttc gacgcgctgg tcgcacaggt cggcctcacc ccggtcaccg
    15781 atgtcgacgg gttcgcgtcg atgcaggcag gcctcgccga acgacgcgac gagctcgatg
    15841 ccctcgccgc cggatccgac gccgaggcat cggatctgga acaggaacgt cgggcgctcg
    15901 acgccgaagc cacgcagatc aacgaggagc tgcgcagcct ccggggtcgg cagggaaacc
    15961 tgccgcgacg atccaccgag atccgcgccg agctctgtcg ggcgctcggc gtcgaggccg
    16021 agctgctgcc gttcgtcggc gagctgctcc aggtccggcc cggcgccgcg gactgggaag
    16081 ggaccgcgga acgtgtcctg cgctcgttcg ccatgtcgct cctcgtcccc gaggagcact
    16141 acggctcggt ggcgacctgg gtcaacgcgc agcacctggg cgccaagctc gtctaccacc
    16201 gtgtgcccga ccggctcggt cgccgcgccg acaccggtcg ctccggaacg gcgccgttgc
    16261 tgctggacat ggtcgaggtg aagcccgagt ccaagttcgc cgactggctc accaacgagc
    16321 tgtggcaccg ggccgatcac gagtgcgtcg acacgatcga ggcgttccgt cggtcgccca
    16381 aggccgtgac ccgtcacggt caggtgaagt cgcgggaccg ccacgagaag gacgaccggt
    16441 accgcgtcga cgaccgacgc aactacgtgc tcgggtggga caacgagctc aaggtcgcgg
    16501 cgttgatcga ccacgcccgt gagctgcacg cccgcggggc cggactcacc gagcgcatcg
    16561 gtgaatgcgc cgagcgtcgg aggatgctga gcgaccgact cggcgacgtg cgggtgctgg
    16621 cggccaggga gcggtgggag gacctcgacc ccgacgcgct gacagcccgc cgcgacgagc
    16681 tggcagccga ggcgacgcgg atccgcgagt cgtcggacgt catgcgggcg ctcaccgacc
    16741 agctcgaggc cgtcgaacgc gaacgtcacg ccgtcggtgt ggagctggcc gatttgcagg
    16801 gccggctcgg agcgcagcgc gaccgttccg atcgggcacg acgacgtctc gacgcgctgg
    16861 acgtcgcggt cccggccgcg atcacggttc cgccgccgct gccgaccggc gccgtcgtcg
    16921 ccggatcgct gttcgatgcc gaacccgaac ccgtgcccgc ggccggaccg gtcggggtgt
    16981 ggctgccggt cgacatcgca gagcgcgcga atgacctcgc caccatcgac gcggcccgtc
    17041 acgacgccgc tggcctgctg gccgagcaga ccgccacagc gcacgatcga cgatcgaagc
    17101 tcgacaaccg cgccgcagcc cagatgcgcg agttcggtca cgcctatccg cgcgaggtcg
    17161 aggagctcga cgcccgcatc gaatccgcag cggagtaccg ggcgatccac gaccgggtcg
    17221 ccaccgacga cctgccccgc ttcgaagccg acttccgtcg tcagctcaag gagaacgcca
    17281 tccacgagat cgccgggctg tcggccgagc tccaccgcca cgaggaggag atccgccggc
    17341 gggtcgaggt gatcaacgcg tcgctcgagg cgatcgacta caacccgggc cgctacatcc
    17401 ggctggtgcc ggccgccacg cccaacaccg agatccggca gttccgcgaa gacctgcgcg
    17461 cctgcaccag cggaatgacc cgcggcaccg acgacgacca gtactccgag gagcggttcc
    17521 tgcaggtcaa ggcgctcatc gaccgtctgc gcggccgcga gggcaccacc gaccaggaca
    17581 gggcgtggcg ccgccgggtc accgacgtac ggcagtggtt cgtgttctcg gcgtcggagc
    17641 ggtggcgcga gggcgacgtc gagcacgaga actactccga ctcctcgggc aagtccggcg
    17701 gccagaagga gaagctcgcc tacacgatcc ttgccgcgtc cctcgcgtac cagttcggcc
    17761 tcggcgacgg cgggtccgga cccgccggcg atgacggatc gttccgattc gtcgtcatcg
    17821 acgaggcgtt cgggcgtggc tccgacgact ccacccgcta cgcgctgaag ctgttcaccg
    17881 agctcggact ccagctgctg atcgtcacgc cgttgcagaa gctgcacgtg atcgagccgc
    17941 acgtggcgtg cgtcggcttc gtcgacaacg tcaacggcga ccgttcgcga ctgcaacgca
    18001 tcaccgtcga ggagctgcag ctccgccgca gcgagcaccg cgctccactc gctgagcccg
    18061 ccgccggtgg agcgccggcc gcaggctcac cggacgggct cggacggtga gcccacgggc
    18121 atcgcggtcg tcgtcggccg acgggtggag ccggcccgag gacatggtcg ccaagctgcg
    18181 cgaccgatgg gatcggggca cgtacctccg ggcccgtgcg gacgcatcac cgtgggagcc
    18241 ggtgcgggtc cgcctcgtcg gtccgaccgc cacggatctg ctcgaacggc cgggcgagtc
    18301 cgttgcgtgg atcgacggcg tacgggcctg ggccgccggc ggtcgggcgg gtccgaggtg
    18361 ccggatcgag gaccggacgg tgcggagccg atcgctgggt gacaacgatg tacccggctt
    18421 cgtcgtggtc gactccatcg aggagctcgc catgctcgtg ggtcggcccg gtcagatcgc
    18481 gacgttcgac cgactgctcg ccctgaccgc cgagcggcga ccggagctcg tgtcgtgggt
    18541 gtcagcgcac ccgatggacg tcctcgaggt cgtggctgac tggttgtcga tgctcgacgt
    18601 cgtcgactgg atcgaccgca ccgacacctc gacggtccac ctgcgccagc tcgacctgcc
    18661 cggcgttgac acgaagttcg tggaacggca ccgcaggctg ctcggccggc tgctccagca
    18721 ggtgctgccc ccggaacggg tagacacctc tgcctcccgc ttcgacgccc gcttcggctt
    18781 ccggaccgtc ccgagctacg tgcggttccg gatcctgtcg cccgtcgagg agctgccggc
    18841 cggcttgagc gagctctcgg tgcgggtcga cgagctcgcc cgactggcgc tcccggtgcg
    18901 cacggtgttc gtggtcgaga accaggtcac ctatctggcg ttcccggacg tgcccgacgc
    18961 catcgtcgtc ttcggcgagg ggttcaaggc gtcgacgctg gatgcggtgc cgtggctcgc
    19021 tgatcgcgag ctcgtctact ggggcgacat cgacacccac gggttccgca tcctcgacca
    19081 gctccgggcg cgggttcccg gcgtgcggtc gctgctgatg gacgagtcga cgctcagcgc
    19141 gcacctcgac cgagtggtca gcgaggacgc tccgacgtcg gtcccgctcg agcacctcag
    19201 ctccgaggag tcggatctct accgggacct ggtcgaggac cggttcggac cgtcggtccg
    19261 cctggagcag gagcgggtcc gcttcggtgc cgtgcgtggg gcgctccgac cgtgggctgg
    19321 tggcgggccc gccggatcgc ccgcggacga ccgcccgggg ttcgggcggg tcggcggaag
    19381 cagcgaccac gttcccgcct gagggggcgc caccgcacgg aacgccccgt gtagcctggc
    19441 ccgtccgcat gaccgacccg accctggacc ctgtgatggc atcgatgctc cggctcccgg
    19501 cgtgtcgtgt cgcggccgat ccggtcacgg tcgaaccacg gggcggagtg gctgcagcgg
    19561 gggccgaggt gccgaggtga ccgccgtccg tcccggccgc agcgtcaccg tcctggtggc
    19621 caacccgtcg tcggacatct acggctcgga cctccagatg ctcgagagca tcaccgcgat
    19681 ggtcgaacgg ggttggaagg tggtggtcgt cacccccgac gacggtccgc tggtggcgct
    19741 gatcgaggaa cgaggggccg aggtcctccg cgtcgactgc gccgtcgtgc gtcgggagta
    19801 cgcgtcgccg tcgggactgt ggagactcgt gcgggagtcg ttcagctccc tgcgaaacct
    19861 cctgcgggtc ctgcggaccc tgcggccgac gttggtgtac acgaacacga tcaccatccc
    19921 gtggtggttc gtcgccgccc gtctctgtcg catccccgtc gtgtgccacg tgcacgaggc
    19981 cgaggccgag gacccgctcc cggtccgact ggtgctctac gggccgttgc tgctggccca
    20041 ccgcctgatc gtcatcagtc gaccgtcgat ggagacggtg tgcgcgacgg tgccgaggct
    20101 ccgtcgtcgg gcgacgatga tcttcaacgg cgtccgggaa ccgaccgagc cgccccgtcc
    20161 ggccgtggcc ggtccgggcc cgcgacgcct cgtggtgctg ggccggctct ccccgcggaa
    20221 ggggaccgac gtcgcgctcg aagccgtcgc gctgctccgc cggagcggtc gtgacgtcac
    20281 cctcgacgtg tgcgggagca cgttccccgg ctacgagtgg ttcgaggagc agctgcgccg
    20341 tcgtgccgcg gagtccgacc tggacggtgc ggtcgacttc tccggctaca tctctccgat
    20401 ctggccggcg atcgagcgtg ccgaggtgtc ggtcgctccg tcgctgcggg agccgttcgg
    20461 caacgccgtg gtcgaggcgc agctagcgca gcgtccggtg gtcgccgccg cggcgatggg
    20521 tcacctcgag acagtgatcg acggggagac gggtctgctc gtgacgccgg gcgacgcgca
    20581 ggccactgcg gacgcgatcg ctcgcctcct cgacgacggg gaccttgccg cgcgaatcgc
    20641 cgagacggca cgacggaccg ccgaagagcg attctcgctc acccgctacc ggcgggaggt
    20701 cgccgacctg ctcgccgcgg tcatgaaact gccggcgggc ggcagcgagg gacggtccgc
    20761 ctagccctga ccggcgcggg tgaactcgtc gaccagctcg cgtgtgttgc ggatgttcgc
    20821 ccgcgacgac gccccgaaca ccaccgagtg gatgttgggc tgctcgtaga tccactcgag
    20881 cgcctcccgc ggggggatgg cgcccgaggc gaacaccgac atggccacca cccggcagcg
    20941 tcggttctgc agggcgtcct tgtaggcgtc cattccgccc gacatccgga acccgatctt
    21001 gttgatgttg gcgcacacga tcgggttgtc gatcccctga cgttcgagca cgtccaacag
    21061 agccggcagg ttcatggtga tgaagcccgg ctccgcgtcg tagcggtcac gcacgtggtc
    21121 ggcgaagatc cggaacgcct cgtcgacgcc caggcccagg agcaggtcga cgacgatgtt
    21181 ctgcatccag atgaccgggg tccgcagtcc ccggaacatc gccatctcgg cgtcgatgag
    21241 cagggtggcc atgccctcga tgtccttggt ggccatcgag cgtccgcccc ggatcgcggc
    21301 gttgatgaac ccctcctcgg ggaggaaccg acgtacggcg ccgagcatcc cggactcggt
    21361 gacggcgttc gcgtacttgt gggcgtacgg catgcacggg aagaaggtga actcgctgta
    21421 gcgatcgggc tgggcccgga cgcggtcgca cacctcggcg atccggtcgt gggtggtgca
    21481 catgaaggtg tggatgccct cgtcgtacgc ggcgtcgagg acggccatca ccgcgtcgat
    21541 gtcctggaag cgcatcgcct gcgcacgcgc cttctcctcg gacatgtggt tcacgccgaa
    21601 gaactggttg tctccgaaca gcagcttgtc catcacttgc gcgacctcct ggcgctgacg
    21661 agcgcgtcct ttgcacgggt ggcgagtcgg cggacgccct cgagagcccg ccgcagcagc
    21721 tcggccgtga gctggccggt cgaggtccga gacgacgtcg gagcgggagc cggtcccgac
    21781 cccgaggtgg tggtgacgcg gctgcttgcc gcgtcccggg tcatcgtgtc gaggacacgg
    21841 tcggtgaccg ccgcgctcgc gaagtcgttc aggccgtcgg gctgctcctt ggccacgcgg
    21901 tggacgaagt agtcgagctg ggcgctgtac tcctcacccc ggaggtagaa ccagacctcg
    21961 tcggtcaact cggtggtgta gcggacgttc cagccgtgct cgtagccggg agggcaggtg
    22021 gcggtgtcgc gcaggtacac ctggcactcc tggcggtcgg cgaagatccg tccctcggtg
    22081 ccccacaacg tcacgcgggt ggtcatcttg cggtacgact cgtcggacca gtcgaccgac
    22141 agctgcgccg aggatccgtc ggggtagtgc agggtgctgt acacctcgtc ctcggtctcg
    22201 gccgagaaga cctggccgag cacggttccg ccgaccgagc tgggcgcacc caggtaccac
    22261 gacagcaggt cgaccggatg ggcggcgtag tcgtacaggc agccgccgcc ttcggagcgc
    22321 tggctgcgcc aggtgccgcc cttgggcttc agcacgaccg ggccgtacgc ctcggccagg
    22381 gcgtgggtga cccggccgat ggcgcccatg tcgagcaggt tccgcacctc gcggaaggct
    22441 ccgacgaagc ggttgtggta gccgacctgc gtgacgagac cccgcgacgc ggcgagctcg
    22501 gcgagcgact ccgagtcctc cggggtcagg cacagcggct tctcgcagaa gacgtgcagg
    22561 ccgttctcca acgcctcgcg aaccatctgc acgtggagcc gggtcggcgt cgcgatgacg
    22621 accgcgtcga gctccacctc ctggagggcg cgactcatgt cggtgaacgt gcggagcccg
    22681 ctgtacttcg acagcacgtc gaccatgtag ggggtcgagt cgaccacggc ggccaactcg
    22741 acctcggggt gggcgcggag catcgcgagg tgcgacagcc ccatcttgcc caggcccacc
    22801 acggcgatcc tgatcatcgg tgacctccag tcgtgcatgt cgcggagtcg ttcacgggct
    22861 tgcgcactcc tccgacccgg acgagcaggt cttcgtactg gcctgcgacg tgggtccagg
    22921 tgaactcggc gaggtgccgg tcccgtgccg cggcgctcag gcggtcgcgg agggctcgat
    22981 cctcgaggag ggaggtgata gcggcgtccg cgtcgtcctc ggttcggaag tagagggcgc
    23041 cgtcggcggc gacccagcgg ttgtagtcgt tgtcgtgtgc gacgatcgcg ttgccggccg
    23101 ccatcgcctc gaccagcgaa gggttggtcc cgccgacggt gtggccgtgc aggtagccga
    23161 tgccgtggaa ccgcagtgcc gtcaccgagt cggggtcgta gatggcgccg acgaagtcga
    23221 cctcgtcgga cgccgcgtcc atgaccgccc ggtggtactc gtcctcgtcc ggtcggtagg
    23281 tgccgagcac caccaggcgc accccgcgct cccgtcggga gaaggcgcgg acgagctcga
    23341 ggatcgtgtt ctccgggatc ggccgacaga tcagcgtgag ataggcgccc ggctccaggc
    23401 cgagcgcggt gaccggcgcg gtgctcgcct cggtgaccgg gtgcgctccg taggcgatcg
    23461 tggtgatctt ccgctcgggc gccctggtcg cgaggtggac ggcgatctcg ggatgatctg
    23521 cgatgagggc gtcgccgacc acgcacgcga tccgctcgtt ggtccagagg atggactgcc
    23581 gggcacgacc ccagcgggcc cgggaccact cgatgccgtc catgttgatg acgttgggga
    23641 tgcgcaacag ccgctggagc gtgttgaaga tcgcggtgtt gtagccgaag gtcaggcaga
    23701 ggtcccggtg acggacggcg tggaggatgg accgcaggtc gaacagcgac gtgcccagcc
    23761 acccgtcgag ggggacggga acgatgaccc gctcgattcc gttccagacg tcggtgcgga
    23821 tccgcccccg accttcgtcc tggcagtaga cgacgacccg ccacccctgg tcgcggagga
    23881 acagggcgac gttctcggcc gcggtctcga acccgccgta gttggcgggg acgccgtgag
    23941 ttccgaggat gcggactgtc ggttgggtcg cgcggagggt tgcggtcatc tgtggggggt
    24001 gggccggaag gccggtcggc ctgttgatcg gtgcggtcag gctaacggcg ggcctgttcg
    24061 cttccaccat tgcgtgatcc acgctgctcc tccggccggc agcggagagc ctcgcggtgt
    24121 cttcgaccag ctccgacctg cagcctgacc gatggtccgg ggtcagatct gcggtcaggt
    24181 gttgagacgc acgccccacg aggtggtcga cgatgctccg ggttcgaggc ggatcacgtc
    24241 ctcgccggtc gcgaacgcgt tgggaggaca cgtcatcggc tcggtgccga gtccccggcg
    24301 acacttgtcg ggtgggagcg tgtcgcccgt gtagatctcg atgtacgggt ggtgacggtc
    24361 gacccacagc gcggcttcgg cgccgtcggt gccggtcaac cgggtccacg cgcggccatc
    24421 gtcgtctcgg gtgaggtcgg tgaacgcgaa gtccacctgg aggtcgccga tcgcccgggg
    24481 tgagcggaag tcgaacgccg tgccctccac cggttcggta ccggtgggga gttggcggac
    24541 cgcgtcggtc gtgatgcgtg tcgcggcgtc gagctgcaac gtgcagccgt cgatgagacc
    24601 gtcgcccggg gacaggtacg gatgcgatcc gcagccgtac gggcatggcc agtcgccccg
    24661 gttctcggct gtcgtggtca ccgccagccc gtccgacgtg agctcgtagg cgacctcgac
    24721 gtggagggtg aacgggtagc cgggcatcgg gtgcagcgtc gtggccatca ccacgcggtg
    24781 ctcgtcgcga tcgatcccct cccaggatcg ccagcgcagg aagccgtgga tcgcgttgtg
    24841 cttcgcaggt tcggtgagag cggtctggtg gtcccggccg tcgaagcgat agcggccgtc
    24901 ggcgaggcga ttgggccacg ggatcagcgg tgtgccgtgc gcgcccgtgc acatctcgtc
    24961 cagcgggtag gggtcgagga cgtgtcgggc tccgacgcgg tactccctga tgccgccgcc
    25021 gacctcgacg atcgtggcgc gctggtcgcc gtggcggatc tcgaactgct gccctgatgg
    25081 cgggaccgac acggtcaccc tcctggtgcg tgggacacca aggatgccgc cacccggcgg
    25141 tgggctgctg gccgcgtcgt tccggcccct tcgaactcgg cctggcgcag ccccgcgtcg
    25201 aggactcgtc gctgcgcgac ggcgtcgaac tcgtgtggtc gcacctgcag gtgctcgccg
    25261 aactggccga gacgctcggc gtgctcgagc ccgggtgaac tctggtcgct cgtcaggcgc
    25321 tcgaccgtct ggaggcatcg ggccatgcgg ccgttgccgt cgctgaaggg gtggatcatc
    25381 accaggttga gatgggccat cgcggctcgg agcaccgcgt ggtcgtcggt gtcggagatg
    25441 gtgtcgagcg tcgacgcgag ctcgccgatc agctggggaa cgagctcccg atcgggggcc
    25501 tcgtagacga gctcgtggtc ctcctcccgc ttcacccaga tggcacccgg ccgccactgc
    25561 ccgaggatct tggacaggtc atggcggatc atcatgaagt gcagggactt gagcagggac
    25621 tcgtcgatgg acggcggatc cgggtcgcgg gcgatctgcc atgcgggtag ccgaggagac
    25681 tgttgagcac gttgttcacc gcgacgtgct gagcctcatg gccgttggcg tacccctcag
    25741 gcgcttcggt cggggggtcg ggccaagcgt cgacgatggt ctccatctcg tggcggtgca
    25801 gtccgaggag agtgtggaac tcgtcaccgg cgaagtaggc gccgtcgatt gagcgccgaa
    25861 ggcaacgtgc gatgacagcc tcgtcggcgt cgctcatcgg tgcccgctgc tccaagccgg
    25921 ggccgacgtc gagggccagc gtcagagcga ctcggacgct gcggagcggt atctaggcgc
    25981 ggtcggaccg ctcggggctg cacggcccgc acgccgctgg caggtccaat ggtgagttgg
    26041 ctactcgaag gggagctccg ggtgcaccgt tgacgccgca aacgaaacgg gtccgtacac
    26101 ggcttcgtag atggcgctgg cctcgtagac ccgcccccga gagcggccgg tgatctcggt
    26161 gaggtccccg cgttcgacca gcgcgtcgat cgctgcctgc gccgttggcc gggtgacgcc
    26221 gatcgcggcc tccacacggg gcgccgtgac gagcgggacg gcgaacagct gctcggcgag
    26281 ccggacgacc gagttcggtc ggccctcgtc gagcaactcg ttgcggagct gatgctggag
    26341 ctcgacgagg cgaaccgtcc gctcctctga gtcgcgtgac tgctgccgaa cgccgcgaag
    26401 gaagaaggcg atccacggca tcaggtcacc cttctggctc gtgaggagca gatggtcgta
    26461 gtactcgctc cggtgctgct cgaggaaggc cgagaggtac agcagcggct gtggcagcgc
    26521 ttcgcgcagc acgagcatga gcgggatcat cagtcggccg atgcggccgt tgccgtcgag
    26581 gaacgggtgg atgacctcga actggtagtg ggcgacagcg agctggacca ggagcggcag
    26641 gtcggtcgcg tggaggaagc gttcgaggtc ggagagggcg tggttcatct cgtcgggcgg
    26701 aggagggacg aaaacggcgt cgtcgagcgt ggtgccgccg agccagaccg ggcttcgccg
    26761 cagctcgccg gggcggcggt gctggcccct cgctccgtcg aggagacgtt cgtgcatctc
    26821 ccggaagagc cggatggaga tggggaagcc ctcacgcact cgagcgagcc cgtgctccat
    26881 cgccacgatg tagttcctga cctccgtggc gtcgtcggcc gcttcacctg gaagcacctg
    26941 cccggcttcg aagcggagca gctcgctcac gtcggtcttg gtcccctcga tccgggagct
    27001 caggaccgcc tcgagacgaa ggtggggtcc gatgagcagg tgcgggttgg ggatcaggcg
    27061 gcccacgccg ccgaggcggt gcactgcgcc agtcgcctca tcgaggagct tgatgacctg
    27121 gtccgacaag ctgagtcgac gaggcgcttc ggtcgggtgg aacgcccagt acccctcggg
    27181 agccttgtgg acgcggcctc gttcggtcga agtgaacgca tcgggccgca tgtcgctcct
    27241 tcgtgaaagc cggctttcgg aacctccact ctagctaaag cgaagcgcca ttcgttttag
    27301 ttaggggtgg cgagtgaaag tgagttctcc ttagcttaga gaggggcgat cgtggcgagc
    27361 gcctcgtcgg cctcgtcgca aggctgggcg gctcgcttcg cgagctcgtt gactcgttga
    27421 ggtggacgtc gaagcagtgc ggtgggtagc agcggaggtc ctcgtgcagt tcgaagcacc
    27481 cgaaggtgac gagtacgtga tcaccggtgt ggagcgggtc agccagacgt gggtcgtcca
    27541 ctaccagagc cggcgccacc tggaatccgg tgagatcagg catgcacgtg caggcaatgg
    27601 tccgatccgt gtcacgggtg actacacgac gaccgtcgct gatacggcat tgccgacgga
    27661 gcacttcgtt gcggagttcg agtcgcgggg cggggagacc ctggatggtc acccctgaca
    27721 tggagcccga actcggattc gaaccgaggg cctgctgttt acaaggcgtc cttggaccgt
    27781 ccacaccgtg agactgcgtc gactgtagcc cggaaactca aggttttcgc gtccacgccg
    27841 tgtcgctcta tccaccggat tcgctcccgc ggattagcaa gggattagca agcggacgac
    27901 cggcccggtg cctgtcagtc ggtgtcgggt ttgccaccac cgagtccagc gacgaacgct
    27961 gcttcggcct gggctccgac cacgagcagg aacaggtcga gcgggagtgt cccgaagtcc
    28021 gatgttgccg acgggtgggc gacggcgact tggggagcga cgaggtaggt gttccgggcg
    28081 aatctcgggt gtcggtcgac gagggcgcgc atccctgatc gggagtggtc gggtgaggag
    28141 gcgatctcga acgccatcgg gtgctcgggg tgatcgaaga tgagatcgac ctcgtgcttg
    28201 ccgtcgcgcc agtgatgaag gcggacgccg gcgtgtacgg cgagggatcg cagggtggcg
    28261 gcgacgaggt tctccagcag cgctccctgc tccgccggat cgtcgagggg agcgacgcct
    28321 cgctgcaacg cggcgttccg gatcgccccg tcaacgaagt acagcttcct tcctcgcttc
    28381 tgcacggttg cttcccgtcc ggagtagttc gggagcgtga agacgaggaa ggcctgctcc
    28441 aggtaggaga ggtaccgatc gaacgtcggt tgggagagac cgaggtcctt gcagatgttg
    28501 gacggggaca ggatgccggt gatctgcgca gccagcacgt aaaggagacg ttcgagcatc
    28561 atcggactgt ccacgccgaa cgactgcggg atgtccttgt agaccgctcg ctcaacggcg
    28621 tcgcttcgca ggatctgttg cgattcgatc aaccgcgtgc cgtcgtcgtt gggtcggtct
    28681 cggggcaagg tgagcagttc ggggaatccg ccgataagca tgaactgccg acgccatcgg
    28741 gtgacgtcgg ggtcggagcg ggtcccgtgt gggagggccc tgatgctctc gctgaggtgt
    28801 cgatcgacgg gggcgtcctt gccggcgtcg acgagctcga ggaactcggt gaacaggtac
    28861 ggcgtcagat gctgctcggc ccaccgaccg acgccactct cgagtcgccg atcgcgcagc
    28921 gcagccgtgg cactggaggt cgcagcaatc cgaaccggcc agcggtcgtc gtagaacgtc
    28981 ttcaaccact gatcccagcg atcggtgtac acaagctcgt ccagcatcaa gaagaccggg
    29041 ctcgcgtcgg tggcgcccga gctctcgatc accgcccgta ccaggtcgcc gagttgctcc
    29101 tgcagcagca gcgggtggtc cagtcgaagc caccagatcc gcttcgggtc ggtcccgtcg
    29161 ctgatgaggt gacgtaccgt ctggtagagc gcggtggtct tgcccactcg tcgtggaccg
    29221 agcaccaact ggaagcggtg tggctcgtcg tcgcggagac ggcgccacag cagcttggcg
    29281 agagtgcgct cgacgggccg tgccagcgca tcaggtaccc ggccgtcgcg ctgccacgga
    29341 ttttgctcgt cgaagacccg ctgcaggtcc tccggctgag ggcgccagac agacaacgtc
    29401 agccaccttt cgtgaggctt aataagacga tattactcac caacagaatg ctataggtca
    29461 aactacaaag cggtggccca ggcggtgagg cttgtcgacg cccttgccgt gcggcgtagt
    29521 tcgcttgggc caggcaggag cgcagtaaag ggctgccgtg gcggccgcct gcgccggaca
    29581 aggacagcgg cgttaccgac gaggtccggg aagtcgtcgt tgtcgaaccc cgtcgccagc
    29641 gcgtggagcg ccgagtcggc gaggtcgagg tactcgacgc tcacttcgcg agtcgctcga
    29701 gcagcactcg gtcctcttcg atgatctgac gcacgcgctg gcggaacttc ggatcccggc
    29761 gacgctggtc gatcgcggcg gacaaggcgt ccttgatggt ctcgttcacg ctcttgccct
    29821 ccacccgggc gagcgcctcg gcgtccgctg ccagttcgtc agggagccga accgtcatgt
    29881 tcttcgtcat ggtggcacga taccgcgcca ccggatccgc gcacgatggg tggcactcga
    29941 cgtgctcgcg gtgcttcagc caggtgcaga accgacgttc accggcgcag ccgattcata
    30001 ccgatctgcg atgttcatag cccgttgagc taagaatccg gtcctgctca ccgacctgcg
    30061 gggcgtcgac aagccggtgc tgctcaacga gttcggccgg atcgcagccc ggcccggcgc
    30121 gcatcggatc cagagccaca cggcggtgac ggtgagggat ggagcctcct ctacgagaag
    30181 ctccacctcc gggtggtagc tcgccaggtg aggccgggtg ctcaccgtct tgccgcgcca
    30241 ggccgagacc cggttgcgca cgatcgtcat ggttgcccgg tcgagctcga catcggtcca
    30301 gcgaagtccg gccagctcgc cgcggcgcac accggtggtc aggaagagca cccacattgt
    30361 gtccgaacgg tcacgagggt cgaggaagac gtcgtggtct cgccagagtt cttcgacgac
    30421 atctgatcgc tgcctgcaag ccgccaccgg tggctccctg tcgcggtagc gcgtcgggcg
    30481 gggtccatgc agggcgatcg cgccgtcgcg accttggtca tgcatccgac tgcccggaac
    30541 gagcctggga gaagcggtca gctcgccggg cggagaagga ccgcgacttc gaggttcagc
    30601 gcggtggcga ccttctgcaa cgtcgtgagc gtggcgccga cccctccggc ttcgagacgg
    30661 gcgatcgccg cctggctggt tcccatccgc gaggcgagct cgcgctgggt caggcccgcc
    30721 gcctcccgcg cgtcgcggac ctgttcaccg acctcgagcg ccaacttggt cgcggcgtag
    30781 gtagcgtcga actcggcgag ctctgcctcg gacatctggc ccaggcgtcg ggccttcagg
    30841 tcatcgatcg tggttcgtgc catcagtcgt cctcctctgc ggtgtgggcc tcagcgatgc
    30901 agcgggtcat cgccgctcga gcccgggtga tctcttgtcg ctcgttcatg cgctgcttgc
    30961 ggaacaccgt cagcagcacg atcctgcgct cgggcacgaa gaagaacgga atcctccacg
    31021 ccgcattcga catgtcgaac cggagttcga acagaccgtc gcccagtggc ctcgacgccg
    31081 gcatccgcaa tgcactgcca cgatccgcca gccgctcgat gtggggaagc acggtggcga
    31141 actcgattgt gctgagcgtg tcgagccatt cctccacctc tggttcgagc tcgaccgacc
    31201 acatgatctc agaaatgata tcagcgatgg ggggggtagc cgggcagctg tcaggactcg
    31261 ctccaaccgc ccccgcctgt tgcgcaagcg caaccagctg gcgatcgacc gctggtcgcc
    31321 tactggcact caggggcagt catggttccg accgctcctt cactggtgtc gaggcacggt
    31381 gtcaccgctt tgccagtcgt ggagtcgacg atccggcggt caccgagcgc cgacgccagc
    31441 gtcactcgct cgaggtcagc gcagccgccc ttgtagctgt cgacacggaa gcggagccgc
    31501 acctcgtcct cggactcctc cacctcgaca tcggcttcct tgtggcaatg ccagcccacg
    31561 tcgagcgaac gaacgtcagc tgacaccagg acgcgactga tcggctggtc ctgccaaccg
    31621 atcgcacaac cagacgcacc gagggtgccc gtgctgatga ccgcgatgac gaacgctcga
    31681 cacaacgtgc tcggctgcat gatcgtctga cgatcgtatg cccgctccgg ttccaccggt
    31741 ggtctggccg gcccgctgct ggagccgaac ggtcacccct tacctagcac cgtgttcagg
    31801 gctgaccacg ttccgccgag ccccagatcc gaccgcccac caccttcgca gaatgtgcgt
    31861 cggactcctg gatccagtgg gcgtagatgt tcgtggtggt cgacggattg cggtggccga
    31921 ctcgaccgga gacgctgcgc agcgggatgc cggcgtcgag gagctgggtg acctggaggt
    31981 ggcgaaggcc atgcaggtgg atccagctcg gcagctccat gcggccgcgc agtcggctga
    32041 acgctctggt cgcgctcgaa gggtgcagcg gcttcagccc gccgggctcg ggtgcgagca
    32101 cgcaggcgtc gttgctcagt tcgatgccgc acagcgcgca cgccttggcg tggcggtccc
    32161 gatggtcgag cagccagtcg agcgcgaacg acgacagagt caccgtgcgg tgctggtgtg
    32221 tcttggtgtc cttctcgtag acgacgttcg tctcgccgat cgccgcctcg atccgcagtg
    32281 tgccggcgtc gacgttgacg tgtcgccagc gcagcgcaca cagctcacct cgacgcgcgc
    32341 cggtgtcggc tgcgagcacg ctcagcatcg ccagcgtcgg gttcaccctc acggcctcgt
    32401 cgatcatcgc tctggccacg tcgatcggca ccggcttcgg gttcgacgtg tgctgccgag
    32461 gaggacgcgc gtcccggccg acgttgaccg cgacccatcc ccagcgcttc gcctggtcga
    32521 gcacccggca caacagcacg tgacagcccc ggaccgtcga gcgcgagtag ccggcctcga
    32581 ccagcgcgcc gtagaagcgg tcgaggtgct cggcgctcag gtgcgacaac cgacgcgacc
    32641 cgagcgccgg cagcacgtgg cactcggccg aggtccggta gctgcgcaag gtcgtcggct
    32701 cgagcccttc gagctcggcc tgacgcaggc ccgcctcgat cgcgtgcgcg acggtgcgct
    32761 ggccgaccga cacctggcca cgagccgact tggtgatcag ctcggccaac gcgacgtcgg
    32821 cctggcggga ggtgccgtgg aaggtcaccg acttctgctg acgcttgcgc gtcaacggat
    32881 cctcacccgc gtcgacgcgc agctcccagg tccccggc
//
LOCUS       DAOQOD010000012        30742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_466_length_30742_cov_11.329488, whole genome shotgun sequence.
ACCESSION   DAOQOD010000012 DAOQOD010000000
VERSION     DAOQOD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 30742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30742
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_30742_cov_11.329488"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="PLZ72_02645"
     CDS             complement(<1..1776)
                     /locus_tag="PLZ72_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA35227.1"
                     /translation="MFLKTLQIKGFKSFADAATLSLEPGVTVVVGPNGSGKSNVVDAI
                     GWVLGAQAPSAVRSQKMDDVIFAGTSKLPQLGRAEVSLTIDNSAGLLPIEFTEVTITR
                     LLFRSGDSEYAINGVPCRLLDIQELLSDSGVGRQQHVIISQGNIDGVLNARPEERRLI
                     IEEAAGVLKYRRRKERAERRLQATEANLTRINDLLREVRRQLRPLERQADAARRHGSL
                     VSELTALRVHLAGRELVRWKGQLEESARTQSALRAEDQRLRATLADLDRRILAAEAQL
                     SAMGGRDVGDLLVRYERVSEKAQGQVALLVERRRNLERELESSVDAAVIASLEAEQHQ
                     LSTDAEDIERDGSAVTEELVDLEALEAELAVLRSDFAEEWGDGVPAPTGEASGVRGEL
                     AALRSAIDRTGADRERLALRQSSLAERVERLAEERIAVVAALEESRDQEPALDSAVAD
                     AATSRAGAEAALAVAEVAATDAETAASAWSARADALSLALDEARQRAGAERLAGLEGV
                     LGTLLDLVDVDAGWAEAFEAAAGEALGAVVVADLDAGRRALSALEAGDHRGAVLALGA
                     RSATSGPGEGTVGVGEPVRRHVRSAA"
     gene            complement(1792..2625)
                     /locus_tag="PLZ72_02650"
     CDS             complement(1792..2625)
                     /locus_tag="PLZ72_02650"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNA35228.1"
                     /translation="MPELPELELLRRDLEREAVDKKIKEADVPGTGLLKGPTKKAFAA
                     ALAGTKITRADRRGTLLVLGIEDTHLLVLSLGERAKLVRAQAKDDMETGTGLVITLTQ
                     GGQLRLVDPKKGSTVELVAADALDEAHPELATLGLDAVDEPISWTTFGEHLLRRDGKL
                     KAVLMDPTFLVGIGPMYSDEILFHAGLRFDRSAATLSAQEIRRLYRAVVETVHEALKY
                     GGTTLGPDGWVQLSGKPGDYTEYLTVYKRDGEMSPRARGPIVKARFGSGYTYYCEQTQ
                     V"
     gene            complement(2618..3427)
                     /gene="rnc"
                     /locus_tag="PLZ72_02655"
     CDS             complement(2618..3427)
                     /gene="rnc"
                     /locus_tag="PLZ72_02655"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNA35229.1"
                     /translation="MVDRSDLDAGDGPSSHRGGEQARRAELCRRIEHRFADDTLLELA
                     LRHRSWCSEHGGVESNERLEFLGDSVLGLVVTEHLFAGSPTSSEGVLARRRSELVSAN
                     ALAGVARSIGLGDVLRLGKGEEATGGRTKTSILADAMEGVIGAVYLDGGLQAARSVVL
                     GLLDERIDGVVSGDVTSDHKSRLQEMAARRFGELPRYEISEDGPEHEKVFRAVVRLAG
                     DRWGAGEGRTKKEAEQAAARETTRLLSQRHDEPAARADGHDADRGEGPPDA"
     gene            complement(3498..3785)
                     /locus_tag="PLZ72_02660"
     CDS             complement(3498..3785)
                     /locus_tag="PLZ72_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNA35230.1"
                     /translation="MPAETHVQQGPVERAEVLQLVKDLLAEILEIDQATIDEGDSFAE
                     DLHASSLALIELVEGLEAELGERSVGFRIEDEDLEDLKTVRDAVDYVVARL"
     gene            complement(4011..4190)
                     /gene="rpmF"
                     /locus_tag="PLZ72_02665"
     CDS             complement(4011..4190)
                     /gene="rpmF"
                     /locus_tag="PLZ72_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNA35231.1"
                     /translation="MAVPKKKTSKAKSRSRRASNWTLKAPSRSLCPRCSAVKQPHVVC
                     GNCGWYGGRQAIDVD"
     gene            complement(4304..4819)
                     /locus_tag="PLZ72_02670"
     CDS             complement(4304..4819)
                     /locus_tag="PLZ72_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNA35232.1"
                     /translation="MAGLRVGVADLRRHPGQRQIIERDVELDGVEVSTAAVPSGSPGH
                     LDVVLESLSDGVAVSGRLSVPWEGPCRRCLEQTSGVEVVELREVFSDSPVDDDLLSID
                     GDVIDLGPVVRDAAVLALPVAPLCGEDCLGPDPDHFPVGTGDGAERAVDPRWSALDEL
                     RFDPGVPDPLE"
     gene            complement(4822..5448)
                     /locus_tag="PLZ72_02675"
     CDS             complement(4822..5448)
                     /locus_tag="PLZ72_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35233.1"
                     /translation="MTAPTDEPTSRPVEPERRPPARSKRSASGDDQYRMPEAQEILER
                     VIAIIDAAPGMPMSASVRVNREELLELLDDAVSRLPDELRSARWLLKEREEFLARTRR
                     EGEDLIEDARSRVAHMVQRTEVVKAAEHHARQIVDEADAVSRRMRRETEDYCDQKLAS
                     FENVLNRIAKSVAAGRDKLAAPRIEEAELAELESRPPADDPAVFDQES"
     gene            complement(5445..5930)
                     /gene="coaD"
                     /locus_tag="PLZ72_02680"
     CDS             complement(5445..5930)
                     /gene="coaD"
                     /locus_tag="PLZ72_02680"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNA35234.1"
                     /translation="MTAVLYPGSFDPIHNGHVEIVETAARLFDRVVVAAMRNPQKGEP
                     LFSLEERQVLIEESLAHLANVEVTMFSSLVVDLARDVGANFIVKGLRAVSDFESELQM
                     AQMNRSISGVDTLFIPSASKNSFLASKLIRDIARFGGNVETMVPGPVADRLKQRFGGT
                     P"
     gene            complement(5927..6550)
                     /gene="rsmD"
                     /locus_tag="PLZ72_02685"
     CDS             complement(5927..6550)
                     /gene="rsmD"
                     /locus_tag="PLZ72_02685"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNA35235.1"
                     /translation="MRVVSGTARGRRLHAPAGLDVRPTLDRVREAMFNALDSMGAIRG
                     ATVLDLFAGSGALGVEALSRGAHHVTFVDSARRSIAAVEANLADLAMSDRATVLRSEA
                     FAALGSSAGSGPFDLVLADPPYAFDQWAELIGALAAVVTPTGVVVAESDRDLEPAVAA
                     SRSTTGAGILRQRRYGGTVVTMLTFEWSPPVPPATASGDLSDQEPGS"
     gene            complement(6623..7399)
                     /locus_tag="PLZ72_02690"
     CDS             complement(6623..7399)
                     /locus_tag="PLZ72_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35236.1"
                     /translation="MTDPSPSDRPTRTRALVWVLVVVATIATIAVVVVFAGDDEPTSA
                     PVNTPSSTTHGGAGHGDDTGSSTTQQYGSHGMAPEVVTPARVGRLGPGPAVGQTFTGS
                     FGLNVCGRFLEPPAAATDAESGMSTDGTGRFQVTPPDADSAGHEADVDELMELIGVDL
                     STGRVTFPATTTPAQFQINNTSMAVAGATFGPDSRCGSVDTVVQVWVYSADAVRTGEG
                     VMAVEVDPEDTPIAQDGMALVVTVTPESSLPTLPPSALAR"
     gene            complement(7446..9659)
                     /gene="recG"
                     /locus_tag="PLZ72_02695"
     CDS             complement(7446..9659)
                     /gene="recG"
                     /locus_tag="PLZ72_02695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNA35237.1"
                     /translation="MSDRPPISLVELSAMDVGVLAGVGPRKLEGLRHLGITSLLDLLT
                     HYPRRYIDRTREAQIRELSEGEEALVLGRVAAVSSRRVRGGRVIVVAELRDDSGSLRC
                     TFFNQPWREKHLAKLSADDAGAEVAVFGRVEVFRGQRQMTNPVLDVIGAGPRARTGRI
                     VAIYPQSDRARLSTWELGEWIAQVLRRSRDRGIDDPVPAEILDRHDLISREEAYAGIH
                     APTSMAHEVVARRRLVFDELFRLQLSLVRRKRQIERDALGIRHVVTASKGTDGPLDRA
                     GRAGLVARYLDGLPHDLTAAQRRAVAEIGTDLASGVPMHRLLQGDVGSGKTVVAMATL
                     LTAVQGGHQGALMAPTEVLAEQHAAGVSRQLGAVVIPDDGGSLLGERPLGVALLTNRT
                     PAAERRRVLAGLADGSVDIVIGTHALIQEGVVFRSLGAVVIDEQHRFGVEQRAALRSA
                     NPDGTVPDVLVMTATPIPRTAAMTVYGDLDTTVLDELPPGRVPISTTWAHSPDQEDSA
                     WQLVREQVAAGNRAYVVCPLVEDSPKLEVASAERTLERLAADELAGLSIGLLHGRMKA
                     SDKAEVMEAFRSGDLDVLVATTVIEVGVDVAEATVMVVLDADRFGMAQLHQLRGRVGR
                     SDRPSWCVLVAHGDLTADAVARLQAMVASSDGFELAEVDLDLRGEGTVLGERQQGLSD
                     LKLASLRHDRQWVEVARDEALRLVGDGGGLDAHPRLAEELDVLLAPEEEEFLLKS"
     gene            complement(9704..10831)
                     /locus_tag="PLZ72_02700"
     CDS             complement(9704..10831)
                     /locus_tag="PLZ72_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35238.1"
                     /translation="MAGPALRTSTWDTPPPDGRSRDSLDATELLEALRAASSVLDQHA
                     DALDRLAAGVEWDSVAGPNPLDPTVDGRSLVSGVDPDDIESDDVEGSNRTEDDVDADS
                     TARRQVAGSDSGPGPGTDMAVTLSGACDAATGAAHFPDLCRGLSDGAHAAARTDVGAQ
                     LAGFLGGAGDALRNADRVEGTRLALALEAGAERVTEGDDGAHPGSLLAVMSAAADGAL
                     DASDAGSDLVEVLVSAAEAGLVELEQGPVADAKLSESGTVDGAAAGFLLVLDSLAAFV
                     SGDPLPEPPRLDPQPAPTPSAGSPRFDVRGRITPPDPGVELAADLEVVIHELSERSSM
                     ERAGGQWIVDATTTLPGAVVEAMSGAGRLSEMHVGLAPGPT"
     gene            complement(10871..11584)
                     /locus_tag="PLZ72_02705"
     CDS             complement(10871..11584)
                     /locus_tag="PLZ72_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35239.1"
                     /translation="MAGADDHSTDHQAGAADHSADHGASDRGTWVLGVLVAAVATFAL
                     AGVVVKAVGADAATVATPAAVSRDHDERSAASEPSAADDPGPDAPPSTVAAAPAAAPT
                     TTPPAVPSTEVPAVSSTTTPTPASVAPPAAGPTTPVPGPAAPSARPGGDGATAAESAA
                     ATTFARLVRQHCDLARVPNPAQVRASDLGDGVFQIVDANATVMQIDVDSNIVMAADSL
                     DGSVSGGYSEACAPALFGG"
     gene            11915..12124
                     /gene="rpmB"
                     /locus_tag="PLZ72_02710"
     CDS             11915..12124
                     /gene="rpmB"
                     /locus_tag="PLZ72_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNA35240.1"
                     /translation="MAAVCEICDKRPSFGMNLSHSHRRTKRRWNPNIQRIRVATPRGT
                     KRMNVCTGCIKSGKVTKPVATRRTA"
     gene            12255..12500
                     /locus_tag="PLZ72_02715"
     CDS             12255..12500
                     /locus_tag="PLZ72_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35241.1"
                     /translation="MIKSYDPVTGDGIVVRDTDLAEHDLAPDALAGSLFRMLRQGQRV
                     RFELDSEGRATGLELGSEVDMGTPVFHAHAEPAPETA"
     gene            12529..14367
                     /locus_tag="PLZ72_02720"
     CDS             12529..14367
                     /locus_tag="PLZ72_02720"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNA35242.1"
                     /translation="MRADRHLTKIDRTGTTMAGTTSNQKLIDWVEEWREIFQPDAVEW
                     CDGSAEEYDRLCQLLVDNGTFEKLSEAKRPNSYLALSDPSDVARVEDRTFIACENEVD
                     AGPTNHWRAPAELKSEMLELYRGTMKGRTMYVVPFSMGPLGSPIAHIGVELTDSPYVA
                     VSMRIMTRMGQGALDVLGSDGDFVPCLHSVGAPLTDGAADVPWPCNDTKYISHFPETK
                     EIWSYGSGYGGNALLGKKCFALRIASTMARDAGWMAEHMLILGITSPDGDKRYVAAAF
                     PSACGKTNMAMLIPSLPGWKVETVGDDIAWMKFGADGRLYAINPEAGFFGVAPGTSDE
                     TNKNAMDTMWGNSIFTNVARTDDGDVWWEDMSEPPAHLVDWKGNDWTPATDTPAAHPN
                     ARFTTPASQCPSIAPEWEDPAGVPISAILFGGRRATNVPLVTQAFNWQHGTFLGSVMS
                     SEKTAAAAGTVGEVRFDPYAMLPFMGYNVGDYIQHWLDIGNATDADKLPQLFWVNWFR
                     KSDDGKFLWPGFGDNSRVLKWALERIAGTADATETPIGWVPTPESLDTDGLDLDADTL
                     EQLLEVDPIAWKGEVQLISNHYDFIGERLPQPMRDELSAMEARLNG"
     gene            complement(14504..14869)
                     /locus_tag="PLZ72_02725"
     CDS             complement(14504..14869)
                     /locus_tag="PLZ72_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35243.1"
                     /translation="MFASVLDVHTEWAWMVILGNALAGLWCLGAHWIPALRVKALWWF
                     VVFVEATIFVQVVLGVIMVAGQGFEAPQFHMFYGFVAIIAVAILYSYRNQMRAHQYLL
                     YGFGGLFLMGLGIRAMLVG"
     gene            14974..15279
                     /locus_tag="PLZ72_02730"
     CDS             14974..15279
                     /locus_tag="PLZ72_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35244.1"
                     /translation="MIHRLHLDDVEMPVRLATLVVVDRGAATVDWEVVAEGLADFTGE
                     LGRIRVEMLCITGANESGQLLLGELCGEAIVVRFVGATVVLRGDGPLHGLSDAVLEG"
     gene            15527..15748
                     /locus_tag="PLZ72_02735"
     CDS             15527..15748
                     /locus_tag="PLZ72_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35245.1"
                     /translation="MDPSNSYVPPISFEELYQAGRLSMPYAEVYEEGYGPLGDPTEVS
                     VELVDPLEAFVDAWAAAGPAPTDALLPRR"
     gene            complement(15841..16665)
                     /locus_tag="PLZ72_02740"
     CDS             complement(15841..16665)
                     /locus_tag="PLZ72_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNA35246.1"
                     /translation="MSTVVCFHAHPDDECIQTGGTMARAAADGHRVVLVVATRGEHGE
                     VVPDVLADGEALWQRRIAETFEAAQILGVQRVEFLGYVDSGMMGDPANTAPYAFWSAD
                     VDAAARRLAAILSEESAEVLTAYDHHGGYGHPDHIQVHRVGLRAAELAGTPVVLQATM
                     NRDHVLAGIAEAREAAEAAREVGDEVTTPLDAPDFDIPDDFGEPAANITHAIDVSDLV
                     GAKRAAMVAHASQIAADSWFLAMDDEQFTRAFGTEWFIRTGGTRAEGVPFLESIWA"
     gene            complement(16685..17851)
                     /locus_tag="PLZ72_02745"
     CDS             complement(16685..17851)
                     /locus_tag="PLZ72_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNA35247.1"
                     /translation="MGPLSGIRIIEVAGIGPGPFAAMMLADMGADVIRVDRTANAMGG
                     DPANPPADILNRGRRSIAVDLKSPEGVAVLLELVASADGLIEGFRPGVAERLGFGPDA
                     CAAVNPKLVFGRMTGWGQEGPYAPTAGHDINYIALAGVLAHLGREGEKPTPPINLVGD
                     FGGGGMLMAFGMVCALLEAQRSGAGQVVDAAMVDGSATLMMMMWAFSAMGIWSEERGT
                     NLLDTGAHFYDTYECADGGFVSIGSIEPQFYAQLLELSGLEAAYRDRGEELPHQMNKA
                     EWGAMKDRLAEIFRTRTRDEWCEVMEGSDVCFAPVLTMSEATRHPHNVARGSFVDVAG
                     IVQPAPAPRFSRTTAEVQRPPAHAGQHTDEILGDWLGLDGDGIAARRASGAVAG"
     gene            complement(18071..19249)
                     /locus_tag="PLZ72_02750"
     CDS             complement(18071..19249)
                     /locus_tag="PLZ72_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA35248.1"
                     /translation="MSTPSGGARRLGVRTALVDGEWIAGDVEVAADGTVAAVGVGGGS
                     GAVGTGVAIPGLVDLQVNGFGGVDLRHADADGYRVAGEALAAHGATSVQPTFFGLSVD
                     GYRDALETLAEIRAEQQGALRDGRPTGCRFLRAHLEGPFLNARFKGAHDERTFLDPSI
                     EVADTLVSSGEVGFVTVAPELPGGLDLVRHLVERGVVVSIGHSDADDIWTRAAMEAGA
                     RHLTHCWNAHRRLTSRDPGPAAVALARTHLVVGLIADGVHVADEIVRLTLHAARGRVA
                     ATTDAIAPAGLVGADGPSDGWDVGDGLAVTVRDGRATLPDGTLAGSVATPDGILRNLL
                     RCGADLPVAVDACGGVQRRLLGLDDVRVRPGDVADLVVLDDTMSVHRALVAGHEVHRA
                     "
     gene            complement(19246..20328)
                     /locus_tag="PLZ72_02755"
     CDS             complement(19246..20328)
                     /locus_tag="PLZ72_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA35249.1"
                     /translation="MSIRVGFLGAGLIAAYHAIQLNELRDRAAADPGSAIPPHRIVSV
                     FDPDRDRAAGLAGGQGAEVAADPDEVIDASDVVFVCTWTSEHLPLVRRVAAAGRALFC
                     EKPLSVDLDRSRELVDVVTDAGLVNMVGLVMRSAPTLLVLREMVDDPASGRVMNVVFR
                     DDQYIPTQGMYASTWRGDRSLAGSGALMEHSIHDLDLLEWLFGPVGSISARQSFFHGI
                     DGIEDSVGALIGFDSGATGSLASIWHDVLSRPSQRRMEVFCENATITLVSDDIGPVIR
                     QSSDGEEVVGGDDLIPWLAARGVATPSAEEELLRAVRTQLDGGTAPRLRPDIDDALRA
                     HVLADATYRSAAAGGAPVSVPAVPSR"
     gene            20389..21294
                     /locus_tag="PLZ72_02760"
     CDS             20389..21294
                     /locus_tag="PLZ72_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA35250.1"
                     /translation="MDVNFGVHTGPANTTVDELRSLWRRIEELPFDWISIWDHFYAAD
                     GRSTNCLDAVVAHTALALETSRVRCGSLVYCAGYRHPGVLANAIAGIDQLSGGRVDVG
                     VGAGWLADEYHAYGIPYPAAGRRLDLMEEYLRCLRGLLREESFSFDGELFQLRDAVVD
                     PRPVQGAVPIWVGGGGERRTLRIAAELADGWNVPFIGPDDFERKVRVLAAHCDDLGRD
                     PAEIVCSVNVGCAPDDESLHRQFGDIAEFVRPGVLMGSTGQMVDAIGRYADAGATRIN
                     LALRAPFEVAALEHLAGAIDQLGAS"
     gene            21333..22235
                     /locus_tag="PLZ72_02765"
     CDS             21333..22235
                     /locus_tag="PLZ72_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35251.1"
                     /translation="MRTDPLTGDTTVVVGSRQGRPNRPSEDCPFCVGGLEAPEPYVVR
                     GFVNRWPAMADDRCEMVLYTPDHDAEFWSLGAAGARAVVDHWAERTAALGARDDVDYV
                     LVFENRGAEVGATIPHPHGQIYAYEHVPPVPLRELVDGVLLPEEVPDDLVVGTHGDWV
                     TWVPFAPTWPYELRLAPRREVPDLLDVRCDRDGLAAALVDGLARLDQLFDEPMPYMLW
                     VHQRPTDGGDWPGARVHVHVCPLNRAAGVARYVAAAEQGGGVWFDPVLPEEAAARLRA
                     LPGMPSGSASAGGSPVSTDRAAGR"
     gene            22232..23242
                     /locus_tag="PLZ72_02770"
     CDS             22232..23242
                     /locus_tag="PLZ72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNA35252.1"
                     /translation="MTARVFAPGRVNLIGDHTDYMGGVVLPMAIQFGTTIVGTAGGGR
                     LRMRSDRLDGVLDVPMPVTDPGSVQPSWGRYPAGVAALLGRTDGFDGEIVSDLPIGGG
                     LSSSASLEVATAHALGATGSPLDIARLCRAAEAAATGVPCGIMDQLAVAAGVEGHALL
                     IDCATEEVTPVPVPSAAAIWVVDSGQVRTLADSAYAERRAQADAAAALVGPLPRASMA
                     SIASVADPVLRARARHVRTECDRVHAAAAALVAGDLVRVGELMVESHVSLRDDFQVST
                     PELDALVEDLVQTPGVHGARLTGAGFGGNVVVLAEDDVTVPGTRMHASAGARTLAAPP
                     NS"
     gene            23330..24025
                     /locus_tag="PLZ72_02775"
     CDS             23330..24025
                     /locus_tag="PLZ72_02775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNA35253.1"
                     /translation="MHDRTPDPDLTSLDRLGPRIPAEVLEACNRLPTKPDPVVLIGER
                     VRVRPATLDDAVELFTISNGEPVTRLGRSTEAYDADELVWRFMPVGPFRSPETYAEHL
                     GSILDRPDWQTFVVEDAATGEALGSASLIANSPTDLKVEIGALWCTPAVQGLGVIQEA
                     CLLQIEHLLTLGYQRIEWKCHAGNARSRAAAVRLGFRFEGIQDHHMVMKGRRRDTAWF
                     RILADERAQTDGG"
     gene            complement(24007..24660)
                     /locus_tag="PLZ72_02780"
     CDS             complement(24007..24660)
                     /locus_tag="PLZ72_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNA35254.1"
                     /translation="MAYDRSNLHANEQIVVDEHPHWIMLMWSVVWVVLAIAFGIFLLV
                     QGWTGWFGTGTKWVAILALVVSLLYLAQRLIKWYSTNFVITTDRCIYREGIISKRGIE
                     IPLERINTVFFHQHLFERLVRAGDLMIESAGTGGEQRFEDIRNPVMVQNLLYQTMEDN
                     ENRKFDRIRTPAEGPGAGGSVADEIAKLAALRDQGHLTDAEFAAQKAQLLARQPPSV"
     gene            24735..26714
                     /locus_tag="PLZ72_02785"
     CDS             24735..26714
                     /locus_tag="PLZ72_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HNA35255.1"
                     /translation="MAPETDRLLRGMNEHQVAAVLSDASPLRILAGAGSGKTRVLTHR
                     IAHRSEAGTLDPSRVLAVTFTRKAAGELRERLGRLGLRDGVHAGTFHAIAYAQLRQRW
                     EERGITPPELLDRKFAIVARLLPGRGRKDDRSTPLDVVAEIEWAAARRITPQDYEREA
                     TRARRRPPVPLGTVATVYDDFTQLKRRRRLVDFDDILRLAARDLEADPVYAAARRWRF
                     RHIFVDEFQDVNPLQFHLLQQWLGPDSDTCIVGDPNQAIYAWNGADAGYLIDFDRYFP
                     GGSTVTLEDNYRSTPQILAVANGVLQQGPSVAIRLRPHRPAGAVPTVRALPDDTAEAR
                     AVARACRDGHRPGVPWSAQAVLVRTNAQTALIAEAMSAAGVPHRVRGSGRLLEQPEVT
                     DALQVLRRSASLEIGLADLEHDLGDRSRLTEERAANVTEVVRLGREYLALEPAGDTAA
                     FLAWLSTTLRDEDGGGGDAVDIVTFHAAKGLEWPVVHIAGLEEGYVPIHHAETDADVD
                     EERRLLYVALTRARDELHCTWARSRTFGSRTMNRSPSMWVDSIETTLGVAPTELSRAQ
                     GAERARSARRSVRRAPAAEGPELELFEALRSWRRDQARAADVPAFVIFNDATLTAIAR
                     HRPRSAAQLLKVPGIGPVKAQRFGPDVLRIVADAT"
     gene            complement(26761..27540)
                     /locus_tag="PLZ72_02790"
     CDS             complement(26761..27540)
                     /locus_tag="PLZ72_02790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA35256.1"
                     /translation="MQDDLEGSGPGAAAGGADAAPAVASDTATEEGGFASPRIEGLAA
                     LELTPRRVEVRRLAQAMRDIIESMVSTTADTDELAAAADDLERLASALGRLPSGQTYE
                     GFAEAAIAGTLPDGPERWAFFDHSPFIGLANPMSPPMTLSYEGDRVVCDVTFGSAYEG
                     PPGCVHGGYVAAAFDELLGAAQSLSGTQGMTAHLGVDYRRPTPLHTPLRMEGWLHRRE
                     GRKIWARATITADGELRAEAEGLFIAFDPGTFQQLLEERSR"
     gene            complement(27557..28336)
                     /locus_tag="PLZ72_02795"
     CDS             complement(27557..28336)
                     /locus_tag="PLZ72_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNA35257.1"
                     /translation="MLIATWNVNSLNARMPRVEEWLATVRPDVLCLQETKMSDAQFPG
                     MDFQRLGYDSVHHGEGRWNGVAILSRVGIDDVVAGFDDGGEPDPDARMLWATCGGVRV
                     GALYVPNGREVGHDHYAYKLAWLGRLRAHLDAHHSPDEALVLLGDWNIAPTDQDVWSP
                     AAFEGSTHVTVEERAALGAVVDWGLVDTLRRRYPQDGIYSYWDYRNGDFHKRRGMRID
                     YLLSSTSLAGRATADLVDRNARKGSKPSDHAPVMAVYDIGT"
     gene            28455..>30742
                     /locus_tag="PLZ72_02800"
     CDS             28455..>30742
                     /locus_tag="PLZ72_02800"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNA35258.1"
                     /translation="MNDSPEPAGTASPGTTDPVASEPLGPNAWLVEEMFEQYQADPGS
                     VSETWRDFFADYRPPGRPAPVATPATPPSTTAPVGTAPPAPTAPPSTAPSTSSTAGPV
                     DEPGDPIRGVGARIVSNMEASLEVPTATSFREVPAKLLEINRSVINGYLGRTRGGKVS
                     FTHIIGYALVRAIADHVPVMNNHFVEGSDGTPRLVRREHVGLGLAVDMEKSDGSRSLV
                     VPVIKDADTMDFAGFSAVYDELIRKAKTNKLTVDDFQGATITLTNPGTIGTERSVPRL
                     MPGQGTIVGAGALGFPAAYAGADPRTLAELGMSKVLTLSSTYDHRIIQGAESGLFLKK
                     VEELLLGEDGFYDDLFRSMGVPYEAVRWRQDAVGDEGRETAQVVKQMQVSTLINMYRV
                     RGHLIADLDPLAAKAPEMHAELDPATYGLTIWDLDREFLTGSENGIYAPVGGVPRQRL
                     GDLLHVLRDAYCRTIGIEFGHIQEPAEKRWIQEQVEGRSSRVDVEDQRHILGRLNAAE
                     ALEKFLATKYLGQKRFGLEGAESLIPILDALLQEAADHGMDSAYLGMPHRGRLNVLAN
                     IVGKSLDQLFREFEGYVDPDSIQGSGDVKYHLGQEGTFVSRSGAELPVSLAANPSHLE
                     AVDPVVEGMTRARMDEIPHDRTRRFPVLPILMHGDAAFAGQGVVAETLNLSLIKGYRV
                     GGTIHVIVNNQLGFTTPPESARSSEYSTDIAKAVQAPIIHVNGDDPEACVRVARLAFA
                     YRQRFHKDVVIDMVCYRRHGHN"
BASE COUNT         4481 a        11321 c        10684 g         4256 t
ORIGIN      
        1 ggcagcggac cgcacgtggc ggcggaccgg ctcgccgacg ccgacggtcc cctcgccggg
       61 acccgacgtc gcggaccggg cgccgagggc caggaccgcc cctcggtggt caccggcctc
      121 gagcgcggac agcgctcggc ggccggcgtc gaggtcggcc accacgaccg cgccgagtgc
      181 ctcgccggcg gcggcttcga acgcctccgc ccagcccgcg tcgacatcga cgaggtcgag
      241 gagggttccc agcactcctt cgagtccggc cagccgctcg gcgccggcac gctgacgggc
      301 ttcgtcgaga gcgagcgaca gcgcgtcggc tcgggccgac cacgccgacg cggcggtctc
      361 ggcgtcggtc gcggcgacct cggcgacggc cagggcggcc tcggcacctg cccgggacgt
      421 ggctgcgtcc gcaaccgccg aatcgagtgc cggctcctgg tcgcgcgact cctccagggc
      481 cgccaccacc gcgatgcgct cctcggccag gcgttcgacc ctctccgcga gcgagctctg
      541 tcgaagggcg aggcgctccc ggtccgctcc ggtgcgatcg atggcgctgc gcaacgcggc
      601 gagctccccc cgcacccccg acgcctcacc ggtcggggcg gggaccccgt caccccactc
      661 ctcggcgaag tccgaacgga gcacggcgag ctcggcctcg agcgcttcca gatcgaccag
      721 ctcctcggtg accgccgatc cgtcgcgctc gatgtcctcg gcgtcggtcg acagctggtg
      781 ctgctcggct tcgagcgacg cgatcaccgc cgcgtcgacg gacgattcga gctcgcgctc
      841 gaggttgcgg cgccgttcga ccagcagcgc gacctgcccc tgagccttct cggagacccg
      901 ctcatagcga accagcaggt cgcccacgtc gcgaccgccc atggccgaca gctgggcctc
      961 agcagccagg atccgacggt cgaggtcggc cagggtggcc cggagcctct ggtcctcggc
     1021 ccgcagagcg gactgggtcc gggcggactc ctcgagctgg cccttccacc gcaccagctc
     1081 ccggccggcg aggtggaccc gcagagccgt cagctccgag accaggctcc cgtggcgtcg
     1141 agcggcgtcg gcctgacgct cgagcggacg cagctgccga cggacctctc ggagcaggtc
     1201 gttgatccgg gtcaggttgg cctcggtggc ctgcagacga cgttcggccc gctccttgcg
     1261 gcggcggtac ttcagcacgc ccgccgcctc ttcgatgatg aggcggcgct cctcgggccg
     1321 ggcgttgagc accccgtcga tgttcccctg cgagatgatc acgtgctgct ggcggccgac
     1381 accgctgtcg gagaggagct cctggatgtc gagcagccgg cagggcaccc cgttgatggc
     1441 gtactcgctg tcgccggagc ggaacaggag ccgggtgatc gtcacctcgg tgaactcgat
     1501 gggcagcagc ccggccgagt tgtcgatggt cagcgacacc tcggcccgtc cgagctgggg
     1561 cagcttggac gtgccggcga agatgacgtc gtccatcttc tgggatcgga ccgctgaggg
     1621 cgcctgggcc ccgagcaccc aaccgatggc gtcgaccacg ttggacttgc cggacccgtt
     1681 ggggccgacg accaccgtga ccccgggttc gaggctgagg gtggccgcat ccgcgaagga
     1741 cttgaaaccc ttgatctgca gggtcttgag gaacacggtt ctcccggggg ctcagacctg
     1801 ggtctgttcg cagtagtagg tgtacccgct gccgaagcgc gccttgacga tcggaccccg
     1861 ggcccggggg ctcatctcgc cgtcgcgctt gtacacggtg aggtactcgg tgtagtcgcc
     1921 gggcttgccc gacagctgga cccagccatc ggggcccagc gtcgtgccac cgtacttgag
     1981 cgcctcgtga acggtctcca ccacagctcg gtagagccgg cggatctcct gtgccgacag
     2041 cgtcgcggcg gagcggtcga agcggagccc ggcgtggaag aggatctcgt cggagtacat
     2101 gggcccgatg ccgaccagga acgtcggatc catgaggacc gccttcagct tgccgtcgcg
     2161 gcggaggagg tgctcgccga aggtggtcca cgagatcggt tcgtcgacgg cgtcgagccc
     2221 gagcgtggcc agctccgggt gcgcctcgtc gagggcgtcc gccgcgacca gctcgacggt
     2281 cgagcccttc ttggggtcga ccaggcgcag ctggccaccc tgggtcagcg tgatgaccag
     2341 gcccgttccg gtctccatgt cgtccttggc ctgggcgcgc accagcttgg cccgctcgcc
     2401 caggctcaac acgagcaggt gcgtgtcctc gatgccgagg acgagcaacg tgccccgacg
     2461 gtccgcccgg gtgatcttgg tcccggccag cgcggcggcg aacgccttct tggtcggccc
     2521 cttgaggagg ccggttccgg ggacgtcggc ctccttgatc ttcttgtcga ccgcctcgcg
     2581 ctccaggtcg cggcgcagca gctccagttc gggcaactca ggcatcgggt ggtccctccc
     2641 cacgatcggc gtcgtgtccg tcggcgcggg ccgcgggttc gtcgtgccgc tggctcagga
     2701 gacgggtggt ctcccgggct gcggcctgtt cggcctcctt cttggtgcgg ccctcgccgg
     2761 ccccccagcg atccccggcc aggcggacca cggcacggaa gaccttctcg tgctcggggc
     2821 cgtcctcgga gatctcgtag cggggcagct cgccgaagcg acgggcggcc atctcctgga
     2881 ggcgtgactt gtggtcgctg gtcacgtcgc ccgacacgac gccgtcgatg cgctcgtcga
     2941 gcagaccgag cacgacgctg cgggcggcct gcagccctcc gtcgaggtag acggcaccga
     3001 tgacgccctc catggcatcg gccaggatcg aggtcttggt gcgaccaccg gtcgcctcct
     3061 cgcccttgcc gaggcggagc acgtcgccca gtccgatcga ccgtgccacg ccggcgaggg
     3121 cgttcgcaga gacgagctcg gagcggcgtc gggcgagcac gccttcgctg gatgtcggcg
     3181 acccggcgaa caggtgctcg gtgaccacca ggcccaggac cgagtcgccc aggaactcca
     3241 gacgttcgtt ggactcgacg cctccgtgct cggagcacca cgagcggtgc cgcaacgcga
     3301 gctccaacag ggtgtcgtcg gcgaagcggt gctcgatgcg tcggcacagc tccgcccggc
     3361 gggcctgctc gccgccgcgg tggctcgagg gaccgtcgcc ggcgtcgagg tcggatcggt
     3421 cgaccatcgg gctgtcctgc accgggatgc ccggggtcgt cgacgttctc ggggtcggag
     3481 gcgccgccgc gggcgcctca gagccgggcg acgacgtagt cgacggcatc acgaacggtc
     3541 ttcaggtcct cgaggtcctc gtcctcgatg cggaagccga cgctgcgctc gccgagctcg
     3601 gcctccagcc cctccaccag ctcgatgagg gcgagagagc tggcgtggag gtcctcggcg
     3661 aaggagtcgc cctcgtcgat cgtggcctgg tcgatctcga ggatctcggc gaggaggtcc
     3721 ttgaccagct gcaggacctc ggcccgctcg acgggaccct gttggacgtg cgtctccgca
     3781 ggcacgatcg ctgcgctctc ctcgctcgga cgacggggcg ggttccccgg gtcggtgtgg
     3841 ccggtcactc taaccggtcg acgcggccgg cgacggcgat cgtcgcggcc cgcgaccggc
     3901 gcggtgtgga cgcccgccga acgcggatcg accaccgtcg gaccgatggg tggtcgcggg
     3961 cccggaggtc gcgaccggcg atgcggggtc ccggagggtg cccgggatcc tcagtcgacg
     4021 tcgatggcct ggcggccgcc gtaccagccg cagttgccgc acacgacgtg gggctgcttc
     4081 accgcgctgc agcgggggca caggctgcgc gagggcgcct tgagcgtcca gttggacgcc
     4141 cggcggctgc ggctcttggc cttggaggtc ttcttcttgg gaactgccat cgttggtctc
     4201 tcgcgcgtcg actacagggg cttcgcgtgg ccaccggccg ggacgtacag gatcggccgg
     4261 accatcccga cgggaacggt cgccgtccgc ccggggcagc gggtcactct agcggatcgg
     4321 ggacaccggg gtcgaagcgc agctcgtcga gggccgacca acgggggtcg accgcccgtt
     4381 cggctccgtc gccggtaccc accgggaagt ggtccgggtc cggaccgagg cagtcctcgc
     4441 cacacagcgg agccaccggc agagccagta ccgcggcatc gcggaccacg ggcccgaggt
     4501 cgatcacgtc gccgtcgatc gacaggaggt cgtcgtcgac cggagaatcc gagaagacct
     4561 ccctcagctc gacgacctcc acacccgacg tctgctccag gcaccgtcga cacggcccct
     4621 cccacgggac gctgagtcga ccgctgaccg ccaccccgtc gctcagggac tccagcacga
     4681 cgtcgaggtg accggggctc ccggagggga ccgctgcggt gctcacctcc acgccgtcca
     4741 gctcgacgtc gcgctcgatg atctgacgct gcccggggtg acgacgcagg tcggccactc
     4801 cgacgcgcaa cccggccacg gtcaggactc ctggtcgaac acggcgggat cgtccgcggg
     4861 aggccgggac tcgagctcgg cgagctccgc ctcctcgatc cgcggcgcgg ccagcttgtc
     4921 gcgccccgcc gccaccgact tggcgatgcg gttgagcacg ttctcgaagc tggccagctt
     4981 ctggtcgcag tagtcctcgg tctcgcgacg catccgccgc gacaccgcgt cggcctcgtc
     5041 gacgatctga cgggcgtggt gctcggccgc cttcaccacc tcggtgcgct gcaccatgtg
     5101 ggccacccgg ctccgggcgt cctcgatgag gtcctcgccc tcgcgtcggg tgcgcgccag
     5161 gaactcctcg cgctccttga gcagccatct ggccgaacgc agctcgtcgg gcagtcgaga
     5221 caccgcgtcg tcgaggagct cgaggagctc ctcgcggttg acgcgcaccg acgccgacat
     5281 cggcatcccg ggcgccgcgt cgatgatggc gatcacgcgc tcgaggatct cctgcgcttc
     5341 gggcatgcgg tactggtcgt cgccggaggc ggaccgcttc gaccgcgccg gtggccgacg
     5401 ctccggttcc accgggcgcg acgtcggctc gtcggtcgga gcggtcatgg ggtacctccg
     5461 aatcgctgct tcagccggtc ggcgaccggg ccgggaacca tcgtctcgac gttgcccccg
     5521 aagcgggcga tgtcacggat gagcttggac gccaggaacg agttcttcga cgcggacggg
     5581 atgaagaggg tgtcgacgcc cgagatcgac cggttcatct gggccatctg cagttcggac
     5641 tcgaagtccg acaccgcccg cagtcccttg acgatgaagt tggcgcccac gtcgcgggcc
     5701 aggtcgacca ccagcgacga gaacatcgtg acctcgacgt tggcgaggtg agcgagcgac
     5761 tcctcgatga ggacctggcg ctcctcgagg gagaagagcg gctcgccctt ctgggggttg
     5821 cgcatggccg ccaccacgac ccggtcgaac agtcgggcgg cggtctcgac gatctcgacg
     5881 tgaccgttgt ggatcgggtc gaacgaaccc gggtagagga ccgcggtcac gagccgggct
     5941 cctggtcgct gaggtcgccg gatgcggtcg cgggcgggac gggcggggac cactcgaagg
     6001 tcagcatcgt cacaaccgta ccgccgtacc gacgttgcct caggatgccc gccccggtcg
     6061 tggaccggga cgcggccacc gcgggctcga gatcgcggtc ggactcggcg accacgaccc
     6121 cggtcggggt gaccacggcg gccagggccc cgatcaactc ggcccactgg tcgaaggcgt
     6181 acggagggtc cgccaggacc agatcgaacg gacccgaccc ggcgctggag ccgagggccg
     6241 cgaaggcctc gctcctgagc acggtggccc ggtccgacat ggccagatcg gcgaggttcg
     6301 cctcgaccgc cgcgatcgag cgccgggccg agtcgacgaa cgtgacgtgg tgcgcgccgc
     6361 ggctcaacgc ctcgacaccg agcgccccgg accctgcgaa caggtcgagc accgtcgcgc
     6421 ccctgatcgc acccatcgag tccagcgcgt tgaacatcgc ctcccggacc cggtcgaggg
     6481 tgggccgcac atcgagcccg gcgggagcat ggagtcgacg cccgcgggct gttccggaga
     6541 cgactctcac ccgaccagtg tcacacggac gggggtggag ggcgggaacc ccaccgctgt
     6601 cctgctgctg cggcggcgac cgtcagcggg cgagcgcgga cggaggcagc gtgggcaggg
     6661 acgactccgg cgtcaccgtc accaccaggg ccatgccgtc ctgtgcgatc ggcgtgtcct
     6721 ccggatcgac ctcgacggcc atgacgcctt cgccggtccg caccgcatca gcgctgtaca
     6781 cccagacctg gaccacggtg tcgaccgaac cgcagcggga gtcgggtccg aaggtggcac
     6841 cggccacggc catcgacgtg ttgttgatct ggaactgagc cggggtcgtc gtcgccggga
     6901 acgtgacccg accggtggac aggtcgacac cgatcagctc catcagctcg tcgacgtcgg
     6961 cctcgtgacc ggccgagtcg gcatcgggcg gggtcacctg gaaccggccg gtgccgtcgg
     7021 tcgacatccc cgactccgca tcggttgcgg ccgccggcgg ttccaggaag cggccgcaga
     7081 cgttgagtcc gaagctcccg gtgaaggtct gtcccaccgc gggcccgggt cccagacgcc
     7141 cgacccgggc cggggtcacc acctctggcg ccatgccgtg ggagccgtac tgctgggtgg
     7201 tcgacgaccc cgtgtcgtcg ccgtgtccgg caccgccatg ggtggtgctc gacggggtgt
     7261 tcaccggcgc cgaggtgggt tcgtcgtcgc cggcgaacac gacgacgacg gcgatggtgg
     7321 cgatggtggc gacgacgacc agcacccaca ccagggcgcg ggttcgggtc ggccgatcgg
     7381 acggggatgg atcggtcacc ccccgatcct gccccagctc ggcgaccgga gcccagggtc
     7441 ggaggtcatg acttgaggag gaactcctcc tcctcggggg cgagcaggac gtcgagctcc
     7501 tcggccagcc ggggatgcgc gtccagtccc ccgccgtcgc cgaccaaccg gagggcctcg
     7561 tcgcgggcca cctccaccca ctggcggtcg tggcgcagcg aggcgagctt caggtcgctc
     7621 aggccctgct ggcgctcgcc gagcaccgtc ccctcgccgc ggaggtcgag gtccacctcg
     7681 gccagctcga atccgtcgga gctggcgacc atcgcctgaa ggcgggccac cgcgtctgcg
     7741 gtgaggtcgc cgtgggccac cagcacgcac cagctgggac ggtcgctgcg tccgacacga
     7801 cctctgagct ggtgcagctg cgccatgccg aagcgatccg cgtcgagcac caccatgacg
     7861 gtcgcctcgg ccacgtcgac gccgacctcg atgacggtcg tggccaccag gacgtcgaga
     7921 tcgccggaac ggaaggcctc catgacctcg gccttgtcgg aggccttcat ccggccgtgc
     7981 agcaggccga tcgacaggcc ggcgagctcg tcggcggcca gacgctccag cgtccgctcc
     8041 gccgaggcaa cctccagctt gggcgagtcc tcgacgagcg ggcacaccac gtaggcccgg
     8101 ttgccggcgg ccacctgctc ccggacgagc tgccacgcgg agtcctcctg gtcggggctg
     8161 tgcgcccagg tggtgctgat cggcacccga cccggcggga gctcgtcgag caccgtggtg
     8221 tccaggtcgc cgtagacggt catggccgcc gtgcgcggga tcggggtggc ggtcatgacc
     8281 aggacgtcgg gcacggtccc gtcggggttg gcgctgcgca gcgcggcgcg ctgctcgacg
     8341 ccgaaccggt gctgctcgtc gatgacgacc gctccgagcg accggaagac cacgccctcc
     8401 tggatcaatg cgtgagtgcc gatcacgatg tcgaccgagc cgtcggccag ccccgccagc
     8461 acccgtcgac gctccgctgc gggggtgcgg ttggtgagca gggcgacccc gaggggccgc
     8521 tcgccgagga ggctgccccc gtcgtcgggg atgacgacgg cgccgagctg gcgggagaca
     8581 ccggcggcgt gctgctcggc caggacctcg gtgggcgcca tcagcgcccc ctggtgtccg
     8641 ccctgcaccg cggtcagcaa cgtggccatg gcgacgaccg tcttgccgga accgacgtcg
     8701 ccctgcagca gccggtgcat ggggacgccc gaggccaggt ccgttccgat ctcggccacg
     8761 gcccgccgct gggccgcggt gagatcgtgg ggcagaccgt cgaggtagcg ggcgaccagg
     8821 cccgcccgac cggcccgatc cagcggtccg tccgtgccct tggacgcggt cacgacatgg
     8881 cggatcccga gcgcgtccct ctcgatctgc ctctttcgcc ggacgaggga cagctgcagg
     8941 cggaacagct cgtcgaacac caggcggcgg cgtgcgacca cctcgtgcgc catcgacgtc
     9001 ggcgcgtgga tcccggcata cgcctcctca cgggagatca ggtcgtgtcg gtcgaggatc
     9061 tccgcgggga ccgggtcgtc gatcccacgg tcacggctgc gacgaaggac ctgtgcgatc
     9121 cactcgccca gctcccacgt gctcaggcgg gcccgatccg actgggggta gatggcgacg
     9181 atgcgaccgg tgcgcgctcg tgggcccgca ccgatgacgt cgaggaccgg gttggtcatc
     9241 tggcgctgac ctcggaagac ctcgacccgc ccgaagaccg ccacctcggc tcccgcatcg
     9301 tccgcgctga gcttggcgag gtgcttctcc ctccacggct ggttgaagaa ggtgcaccgc
     9361 agcgacccgc tgtcgtcgcg gagctccgcc acgacgatga cccggccgcc ccggacgcgg
     9421 cgagaggaca ccgctgcgac ccgacccagc accagggcct cctctccctc ggagagctcc
     9481 cggatctgcg cctcgcgggt tcggtcgatg tagcggcgcg ggtagtgggt gagcaggtcg
     9541 agcagcgagg tgatcccgag atgacgcaac ccctccagct tgcgggggcc gacaccggcg
     9601 agcacgccga cgtccatggc cgacagctcg acgagtgaga tcgggggacg gtcgctcacc
     9661 gccccagtcc agcacggtgc accgacgacg tcccgcccgc cgctcaggtc ggaccgggag
     9721 ccaggcccac gtgcatctcg ctgagccgac ccgcacccga catggcttcg accaccgctc
     9781 ccggcagggt cgtggtcgcg tcgacgatcc actgaccccc tgctcgttcc atcgacgagc
     9841 gctcggagag ctcgtggatc acgacctcga ggtcggcggc gagctcgacg ccgggatcgg
     9901 gcggcgtgat ccgaccgcgt acatcgaaac gcggcgagcc cgccgagggc gtcggggccg
     9961 gctgtgggtc caggcggggc ggttcgggca gcgggtcgcc ggagacgaac gcggccaggg
    10021 agtcgagcac gagcaggaag cccgccgcgg cgccgtcgac cgtgccgctc tcggagagct
    10081 tggcgtcggc caccgggccc tgctcgagct cgaccaggcc ggcctcggcg gccgagacca
    10141 ggacctcgac caggtcggag ccggcgtcgg acgcgtcgag ggcaccgtcg gccgcggcgg
    10201 acatcaccgc cagcaggctc ccgggatggg cgccgtcgtc gccctcggtg acccgttcgg
    10261 cccccgcttc caacgccagc gccagtcggg tgccctcgac ccgatcggcg ttgcgcagag
    10321 cgtcgccggc accgcccagg aacccggcga gctgagcacc gacatcggtc cgcgccgcgg
    10381 cgtgggcgcc gtcggacagt ccccggcaca gatccgggaa gtgggcggcg ccggtcgccg
    10441 cgtcacatgc tccgctgagc gtgaccgcca tgtcggtgcc cgggccgggg ccggagtcgg
    10501 atcccgctac ttgtcgacga gccgtcgagt cggcatcgac gtcgtcctcg gtccggttcg
    10561 aaccctcgac atcgtcggat tcgatgtcgt cggggtcgac gccggagacc agcgaccgac
    10621 cgtcgacggt cggatcgagc gggttgggtc ccgcaacaga gtcccactcg accccggcag
    10681 ccaggcggtc gagcgcgtcc gcgtgctggt cgagcaccga ggaggccgca cgcaacgcct
    10741 cgaggagctc cgtggcatcc aggctgtccc gcgaccgacc gtcgggcgga ggggtgtccc
    10801 acgtcgaggt gcgcaacgcc gggccggcca tgaccccagc gtacagaccc tccgtcgggc
    10861 gggcgccgga tcagccgccg aacagggccg gggcgcaggc ctcggaatag ccgccagaca
    10921 ccgagccgtc gaggctgtcg gcagccatca cgatgttcga gtcgacgtcg atctgcatga
    10981 cggtcgcgtt ggcgtcgacg atctggaaga cgccgtcgcc caggtccgac gctcgcacct
    11041 gtgccgggtt ggggacgcgg gccaggtcgc agtgctggcg gacgagccgg gcgaaggtgg
    11101 tggcggccgc ggactccgcc gctgtcgccc cgtcgccacc gggtcgggcc gatggagcgg
    11161 ccggtcccgg aaccggtgtg gtgggtccgg ccgccggggg cgcaaccgag gccggggtcg
    11221 gggtcgtggt gctcgacacg gccggtacct cggtgctcgg caccgcaggc ggcgtcgtcg
    11281 tcggcgctgc ggccggagct gcggcgacag tgctgggggg tgcatcggga ccggggtcat
    11341 cggctgcgga cggctccgac gccgccgagc gctcgtcgtg gtcacgggac accgccgctg
    11401 gggtggcaac agtcgccgca tccgctccca ccgccttgac gacgacgccg gccaaggcga
    11461 acgtcgccac cgcggcgacg agcacgccga gcacccacgt cccacgatcc gatgcaccgt
    11521 ggtcggcgct gtggtccgcg gcgccggcct gatggtcggt gctgtggtcg tctgctcccg
    11581 ccatggcgtt cccctcctcg ttgcggacga cccgctcgtc gtcaccgctc cacgatgaga
    11641 gagtcgactg ccgcgatcaa gtcacgaggc gagattcccc tcacccgatg gtcgcccacg
    11701 agccatgtcc ggacctcggg atgtccagat tgtccggccc gcaacgggcg tgcttgggtc
    11761 gatcgcctcg acgcactaca gtgtccggtc ggccctgcgc cggggttccg ccccgccggc
    11821 cgaacctccc gttcccggga gccgaacccg tagaaggcgc cacccacggt ggcacgccgc
    11881 cgaccgtggc ggaacgaccg gagaagagac agccatggcc gccgtgtgcg agatctgcga
    11941 caagcgcccc tcgttcggga tgaacctcag ccactcccat cgccgcacca agcgccgctg
    12001 gaacccgaac atccagcgga tccgcgtcgc cacgccccgt ggcaccaagc ggatgaacgt
    12061 ctgcaccggt tgcatcaagt cgggcaaggt caccaagccc gtcgccaccc gccgcaccgc
    12121 ctgagcccgc ccgctgcggg tcggtcccgt ccccggcttt gccgcccgtt ctcggccgcc
    12181 ggtacggtga cggtgggtca gcagcctccg gttccggagg ctcaccagtc aggagcggcg
    12241 accgagtgca aggcgtgatc aagtcctatg accccgtgac cggcgacggg atcgtcgtgc
    12301 gcgacacgga cctggccgag cacgacctgg cccccgacgc gctggcgggc tcgctgttcc
    12361 gcatgctccg tcagggccag cgggttcgct tcgagctcga ctccgagggc cgggcgaccg
    12421 gactggagct cggctccgag gtggacatgg ggactccggt gttccacgcc cacgccgagc
    12481 cggcacccga gaccgcctga ccgcggccac ccatccgcac agccgacggt gcgggcagat
    12541 cgacacctca cgaagatcga cagaacgggg acaacgatgg cagggaccac cagcaaccag
    12601 aagctgatcg actgggtcga ggagtggcgg gagatcttcc agcccgacgc ggtggagtgg
    12661 tgcgacggca gcgccgagga gtatgaccgg ctctgccagc tcctcgtcga caacggcacc
    12721 ttcgagaagt tgtccgaggc caagcgaccg aacagctacc tggccctgtc ggacccctcc
    12781 gacgtcgccc gcgtcgagga ccgcacgttc atcgcctgcg agaacgaggt cgacgcgggg
    12841 ccaaccaacc actggcgggc gcccgccgaa ctcaagtccg agatgctcga gctgtaccgc
    12901 ggcaccatga agggccgcac gatgtacgtg gtgccgttct cgatgggacc gctcggctcc
    12961 ccgatcgccc acatcggcgt cgagctgacc gactcgccgt acgtggcggt gtcgatgagg
    13021 atcatgaccc gcatgggcca gggcgccctc gacgtgctcg gctccgacgg cgacttcgtt
    13081 ccctgcctgc actcggtggg tgcaccgctc accgacggtg cggcggacgt tccgtggcca
    13141 tgcaacgaca ccaagtacat ctcgcacttc cccgagacca aggagatctg gtcctacggc
    13201 tcgggctacg gcggcaacgc tctgctcggc aagaagtgct tcgccctgcg catcgcctcg
    13261 accatggccc gcgacgccgg ctggatggcc gagcacatgc tgatcctcgg catcacctcg
    13321 cccgacggcg acaagcgcta cgtcgccgcg gcgttcccgt cggcctgcgg caagaccaac
    13381 atggcgatgc tgatcccgtc gctgccgggc tggaaggtcg agacggtcgg tgacgacatc
    13441 gcctggatga agttcggggc cgacggccgc ctctacgcca tcaaccccga ggccgggttc
    13501 ttcggcgtcg ctccgggcac ctccgacgag accaacaaga acgccatgga cacgatgtgg
    13561 ggcaactcca tcttcaccaa cgtggcccgc accgacgacg gcgacgtctg gtgggaggac
    13621 atgtccgagc cgccggccca cctcgtcgac tggaagggca acgactggac cccggccacc
    13681 gacaccccag ccgcgcaccc caacgcccgg ttcaccaccc cggcgtcgca gtgcccgtcg
    13741 atcgcccccg agtgggagga cccggccggc gtgccgatct cggccatcct cttcggcggc
    13801 cgccgggcca ccaacgtccc gctcgtgacc caggccttca actggcagca cggcacgttc
    13861 ctgggctcgg tcatgagctc ggagaagacc gcagcggcgg ccggcaccgt cggcgaggtc
    13921 cgcttcgacc cgtacgccat gctgccgttc atgggctaca acgtcggcga ctacatccag
    13981 cactggctcg acatcggcaa cgcgactgac gccgacaagc tgccccagct cttctgggtc
    14041 aactggttcc gcaagtccga cgacggcaag ttcctctggc ccggcttcgg cgacaacagc
    14101 cgagtgctca agtgggcact ggagcgcatc gccggcaccg ccgacgccac cgagaccccg
    14161 atcgggtggg ttccgacccc cgaatccctc gacaccgacg gtctcgacct cgacgccgac
    14221 accctcgagc agctgctcga ggtcgacccg atcgcctgga agggcgaggt ccagctgatc
    14281 agcaaccact acgacttcat cggcgagcgc ctcccgcagc ccatgcgcga cgagctctcc
    14341 gccatggagg cccgcctcaa cggctgagcg tcgctcgagc agatctacgc accgcctccg
    14401 gagacctcgg ctctccggag gcggtgtcgc gtccggaccc gcagcgtcac ccgaccagca
    14461 tcgcccggat gctctcccac gcgaagcatc cggacccgca gcgtcacccg accagcatcg
    14521 cccggatgcc gaggcccatg aggaagaggc ctccgaagcc gtagagcagg tactggtgcg
    14581 cccgcatctg gttgcggtag ctgtacagga tcgccaccgc gatgatggca acgaacccgt
    14641 agaacatgtg gaactgcggc gcctcgaacc cctgaccggc caccatgatc acgccgagga
    14701 ccacctgcac gaagatcgtc gcctcgacga agaccacgaa ccaccacagg gccttgaccc
    14761 tcagcgccgg tatccagtgc gcccccaggc accacagacc cgccaacgcg ttgccgagga
    14821 tgaccatcca ggcccattcg gtgtggacgt ccaggaccga ggcgaacatc gtcgggacac
    14881 tagcggcgcg cccacgagcg acttcaacct ccgggggcgg ccaccggacc ggaccgccga
    14941 tggcaagctg agccagtgcg ccacgcccga ttggtgatcc accgactcca cctcgacgac
    15001 gtcgagatgc cggtccgact ggccaccctg gtcgtcgtgg accgcggggc cgccaccgtc
    15061 gactgggagg tcgtcgccga gggactcgcg gacttcaccg gcgagctcgg tcggatccgg
    15121 gtggagatgc tgtgcatcac gggagcgaac gagtcggggc agctgctcct gggagagctc
    15181 tgcggcgagg cgatcgtggt ccgcttcgtg ggcgccaccg tcgtgctccg cggcgacgga
    15241 ccgctgcacg gtctgagcga cgccgtcctc gagggctgaa cccgctccgg cgcgcggctc
    15301 gtcggtgact ccgaaccggc tcggctcacc cgacccgatc cccccggtct tgaggaaacc
    15361 ctcatctatc gccccgcggc tgccgattcg ggattaggtt cgacagcgcc gggatggctg
    15421 cccggtccgc agggcgacgc acgacaccgc tcgacatcac accgagcgac acgacaccga
    15481 gcgacacgac accgatcaag acgacaccga cccaggagca cgcaccgtgg acccgtccaa
    15541 cagctacgtc ccacccatca gcttcgagga gctctaccag gccgggcgtc tgagcatgcc
    15601 gtacgccgag gtctacgagg agggctacgg acccctcgga gatccgaccg aggtgtcggt
    15661 cgagctcgtc gacccgctcg aggcgttcgt ggacgcgtgg gccgctgcgg gtccggcgcc
    15721 caccgacgcg ctgctgcccc ggcgctgacg tccgctcagc gcgccgcgga gacctccccg
    15781 accgtcgtcg cccgctgagt cccgctccgc gggtggcacc gctcaggccc agatcgacgc
    15841 tcaggcccag atcgactcca ggaacggaac gccctcggcc cgcgtgcccc cggtgcggat
    15901 gaaccactcg gtgccgaagg cccgggtgaa ctgctcgtcg tccatggcca ggaaccagga
    15961 gtccgccgcg atctgactgg cgtgggcgac catcgccgcc cgcttcgcgc ccaccaggtc
    16021 gctgacgtcg atcgcgtggg tgatgttggc ggccggctca ccgaagtcgt cggggatgtc
    16081 gaagtcggga gcatccaacg gcgtggtcac ctcgtcgccg acctcccgtg cggcctccgc
    16141 cgcctcccgc gcctcggcga tcccggcgag gacgtggtcg cggttcatcg tggcctggag
    16201 caccaccgga gtgccggcga gttctgccgc ccggagcccc acccggtgga cctggatgtg
    16261 gtccggatgc ccgtagccgc cgtggtggtc gtaggcggtc aggacctcgg cggactcctc
    16321 ggacaggatc gccgccagtc ggcgggcagc cgcgtcgacg tccgccgacc agaacgcgta
    16381 gggggcggtg ttggcgggat cccccatcat cccggagtcg acgtagccga ggaactccac
    16441 ccgctgaacg ccgaggatct gcgcggcctc gaaggtctcg gcgatgcggc gttgccacag
    16501 cgcctcgccg tcggccagga cgtcggggac gacctcgccg tgctcgcccc gggtcgcgac
    16561 caccaggacc acccggtgtc cgtcggcggc cgcccgggcc atggtgccgc cggtctggat
    16621 gcactcgtca tcggggtggg cgtggaagca gacgacggtc gacatcgggc ggcaggctaa
    16681 cgcctcaccc cgcgaccgcc cccgacgccc gtcgggcggc gatgccgtcg ccgtccaggc
    16741 cgagccagtc gcccaggatc tcgtcggtgt gctgaccggc gtgagcggga gggcgttgga
    16801 cctcggcggt ggtgcggctg aaccgcggcg cgggcgcggg ctgcacgatg ccggcgacgt
    16861 cgacgaagga cccccgggcc acgttgtggg ggtggcgggt cgcctcgctc atggtcagca
    16921 ccggggcgaa gcacacgtcg ctgccctcca tgacctcgca ccactcgtca cgtgtccggg
    16981 tccggaagat ctcggccaac cggtccttca tggcccccca ctcggccttg ttcatctggt
    17041 gcggcagctc ctcaccacgg tcccggtagg cggcctcgag tccggacagc tcgagcagtt
    17101 gggcgtagaa ctgcggctcg atcgagccga tcgacacgaa cccgccgtcc gcgcactcgt
    17161 aggtgtcgta gaaatgagct ccggtgtcga gcaggttggt gccgcgctcc tcggaccaga
    17221 tccccatggc cgagaacgcc cacatcatca tcatcaacgt ggcggaaccg tcgaccatcg
    17281 ccgcgtcgac cacctgtccg gcaccggagc gctgcgcctc gaggagtgcg cacaccatgc
    17341 cgaacgccat cagcatcccg ccgccgccga agtcgccgac caggttgatg ggcggggtcg
    17401 gcttctcgcc ctcacggccg aggtgggcca ggacgccggc cagggcgatg tagttgatgt
    17461 cgtggccggc ggtcggggcg tagggaccct cctgacccca tccggtcatg cggccgaaca
    17521 ccagcttggg gttgaccgcc gcgcaggcgt cggggccgaa acccaaccgc tcggcgaccc
    17581 cggggcggaa gccctcgatc agcccgtcgg cagatgcgac gagctcgagc aggacggcga
    17641 cgccctcggg cgacttgagg tcgaccgcga tcgaacggcg gccccggttg aggatgtcgg
    17701 cgggcgggtt ggccgggtcg ccgcccatgg cgttggcggt ccggtcgacg cggatcacgt
    17761 ccgcgcccat gtcggccagc atcatggcgg cgaagggccc cgggccgatg ccggccacct
    17821 cgatgatcct gatcccgctc aacggtccca cgtgcatgcc ccctcggctc cggactcggt
    17881 cgtccgcagg agtgttcccg gggccgaccc cggggtcaaa ccgcgggccg gctcaccggg
    17941 ctccgcgtgt gggatcgacg cgtggcaggt cgatctcaca cgcgagcaac acggcccagg
    18001 ccccgcgtgt gggatcgacg cgtggcaggt cgatctcaca cgcgaacagc gggctccggg
    18061 cgagcggcgt ctacgcccgg tgcacctcgt ggccggcgac gagcgcgcga tggaccgaca
    18121 tcgtgtcgtc caacacgacg agatcggcga cgtcgccggg ccggacccgc acgtcgtcga
    18181 ggccgagcag gcgtcgctgc acgccaccgc acgcgtccac ggccacgggc agatccgctc
    18241 cgcatcgcag caggttccgc aggatgccgt cgggtgtggc cacgctgccg gcgagagtgc
    18301 cgtcgggcag cgtcgcccgg ccgtcccgga ccgtcacggc gaggccgtcc ccgacgtccc
    18361 agccgtccga cgggccgtcg gcgccgacca gtcccgcggg ggcgatggcg tcggtggtcg
    18421 ccgcgacgcg cccgcgagcc gcgtggaggg tgagacggac gatctcgtcg gccacgtgga
    18481 ccccgtccgc gatcagcccg acgaccaggt gggtgcgggc cagcgcgacc gcggccgggc
    18541 ccgggtcccg tgacgtcagg cggcggtggg cgttccagca gtgggtcagg tggcgcgctc
    18601 cggcctccat cgcggcccgg gtccagatgt cgtccgcgtc gctgtggccg atgctgacga
    18661 cgacaccgcg ttcgacgagg tgacgcacca ggtcgagtcc gcccggcagc tcgggcgcca
    18721 cggtcacgaa gccgacctca ccggagctca ccaacgtgtc ggccacctcg atcgacgggt
    18781 cgaggaaggt ccgctcgtcg tgtgccccct tgaaccgggc gttgaggaac ggaccttcga
    18841 ggtgggctcg gaggaaccgg caccccgtcg gtcgaccgtc ccggagagcg ccctgctgct
    18901 cggcccggat ctcggccagg gtctcgagcg cgtcgcggta cccgtcgacc gacaggccga
    18961 agaaggtcgg ctggaccgag gtcgcgccgt gcgcggccag ggcttcgccc gcgacccggt
    19021 agccgtccgc gtcggcgtgg cggaggtcga cgccgccgaa gccgttgacc tgcaggtcga
    19081 ccagcccggg gatcgcgacg ccggtgccga ccgcacccga accgccgccg acgccgacgg
    19141 ccgcgaccgt gccgtcggcg gcgacctcga cgtcgccggc gatccactcg ccgtcgacca
    19201 gcgccgtccg cacgccgagc cggcgggcac cgccagacgg tgtgctcatc gggacgggac
    19261 ggccggaacc gacaccggcg caccgccggc cgccgcggac cgatacgtcg cgtcggccag
    19321 cacgtgggcc cgcagggcgt cgtcgatgtc gggacggagc cggggtgcgg ttccgccgtc
    19381 gagctgcgtc cgcaccgccc gcaggagctc ctcctcggcg ctgggagttg ccacgccgcg
    19441 ggcagcgagc cacgggatca ggtcgtcgcc cccgaccacc tcctcgccgt cgctgctctg
    19501 gcggatcacc ggcccgatgt cgtcggagac cagggtgatg gtggcgttct cgcagaacac
    19561 ctccatgcgc cgctggctcg gccggctgag cacgtcgtgc cagatcgacg cgagcgaacc
    19621 ggtggcgccg ctgtcgaagc cgatgagggc accgacggag tcctcgatgc cgtcgatgcc
    19681 gtggaagaac gactggcgcg ccgagatcga cccgacaggg ccgaacagcc actccagcag
    19741 gtccaggtcg tggatcgagt gctccatcaa cgcccccgac ccggcgagcg accggtcgcc
    19801 gcgccacgtc gaggcgtaca tcccctgggt cgggatgtac tggtcgtcgc ggaacacgac
    19861 gttcatgacc cgacccgatg ccgggtcgtc gaccatctcc cgcagcacga gcaacgtggg
    19921 cgcggagcgc atcaccagcc cgaccatgtt gacgagtccg gcatcggtca ccacgtcgac
    19981 gagctcccgt gaccggtcca ggtcgaccga cagcggcttc tcgcagaaca gcgcccggcc
    20041 cgctgcggcg acccggcgca ccagcggcag gtgctcggat gtccaggtgc acacgaacac
    20101 gacgtcggag gcgtcgatga cctcgtcggg atccgcggcg acctcagcgc cctgaccgcc
    20161 ggccaggccc gcggcacggt cacggtcggg gtcgaacacc gacacgatcc ggtgaggcgg
    20221 gatggcggag ccggggtcgg ccgcggcgcg gtccctgagc tcgttgagct ggatcgcgtg
    20281 gtaggcggcg atcagtccgg cacccaggaa gcccactcgg atcgacatgc ccgcacagta
    20341 gggcgcacgg gtcaccacgg agcggtccgc tggcgtaccg tgccggtcgt ggacgtgaac
    20401 ttcggggtgc acaccgggcc ggcgaacacc accgtcgacg agctgcggtc gctgtggcgg
    20461 cggatcgagg agctgccgtt cgactggatc tcgatctggg accacttcta cgcggcggac
    20521 ggacggtcga ccaactgcct cgacgccgtg gtcgcccaca ccgctctggc gctcgagacc
    20581 tcccgggtgc ggtgcgggtc gctggtgtac tgcgccggct accgccaccc cggcgtgctg
    20641 gcgaacgcca tcgccggcat cgatcagctc tcgggcggtc gggtcgacgt cggcgtcggc
    20701 gccggctggt tggccgacga gtaccacgcc tacggcatcc cctaccccgc cgccgggcgc
    20761 cgactcgacc tgatggagga gtacctgcgc tgcctgcgcg gcctgctccg cgaagagtcg
    20821 ttctccttcg acggcgagct cttccagctc cgggatgcgg tggtcgaccc ccggccggtc
    20881 cagggcgccg tgccgatctg ggtcgggggc gggggcgaga ggcgcacgtt gcgcatcgcc
    20941 gccgagctcg cggacggctg gaacgtcccg ttcatcggcc ccgacgactt cgagcgcaag
    21001 gtccgggtgc tcgccgcaca ctgcgacgac ctcggccgcg acccggccga gatcgtgtgc
    21061 tccgtcaacg tcggctgcgc ccccgacgac gagtcgctgc accgccagtt cggcgacatc
    21121 gccgagttcg tccggcccgg ggtgctcatg ggttcgaccg ggcagatggt cgatgccatc
    21181 ggtcgctacg ccgacgccgg ggccacccgg atcaacctgg cgttgcgcgc cccgttcgag
    21241 gtggccgcgc tcgagcacct ggccggggcg atcgatcaac tcggcgcctc gtgaaccacc
    21301 accgtcccga gtcctccacc gacggaccgc aaatgcggac cgacccgttg accggcgaca
    21361 ccaccgtcgt ggtcggcagc cgtcaagggc gcccgaaccg cccgagcgag gactgcccgt
    21421 tctgcgtcgg cgggctcgag gcccccgaac cctacgtcgt ccgcgggttc gtgaaccgct
    21481 ggccggccat ggccgacgac cgctgcgaga tggtcctcta cacccccgac cacgacgccg
    21541 agttctggtc gctcggagcg gcgggcgcgc gtgccgtcgt cgaccactgg gcggagcgca
    21601 ccgcggcgct cggcgcccgc gacgacgtcg actacgtgct cgtgttcgag aaccgcggtg
    21661 ccgaggtcgg cgccacgatc ccccacccgc acggccagat ctacgcgtac gagcacgttc
    21721 cgccggtacc gctgagggag ctggtcgacg gggtgctgct ccccgaggag gtccccgacg
    21781 acctcgtcgt cggcacccac ggcgactggg tcacgtgggt cccgttcgct ccgacgtggc
    21841 cctacgagct gcgcctggcc ccccgccgcg aggtccccga tctcctcgac gtccggtgcg
    21901 accgcgacgg cctggccgcc gccctggtcg acggcctggc ccgcctcgac cagctcttcg
    21961 acgagccgat gccgtacatg ctgtgggtcc accagcgacc caccgacggc ggcgactggc
    22021 ccggggcgcg ggtgcacgtc cacgtctgcc cgttgaaccg cgccgccggc gtggcccgat
    22081 acgtggccgc agccgagcag ggcgggggcg tgtggttcga tccggtgctc cccgaggagg
    22141 ccgccgcacg gctgcgggcg ctcccgggca tgccgtccgg aagcgcctcg gccggcggca
    22201 gccccgtttc caccgatcgg gcggctggcc ggtgacggcg cgggtgttcg cccccggtcg
    22261 tgtgaacctg atcggagacc acaccgacta catgggcggg gtggtcctgc cgatggcgat
    22321 ccagttcgga accacgatcg tcgggaccgc tggcggcgga cggctgcgga tgcgctcgga
    22381 ccggctcgac ggtgtcctcg acgtcccgat gccggtgacc gaccccggat cggtccagcc
    22441 ctcctggggt cgctacccgg ccggggtggc tgccctgctc ggacggaccg acgggttcga
    22501 cggcgagatc gtctcggacc tgccgatcgg cggcggtctg tcgtcgagcg cctcgctcga
    22561 ggtcgccacc gcgcacgctc tgggtgcgac cggatccccg ctcgacatcg cacgcctgtg
    22621 ccgggccgcc gaggctgccg cgacgggggt cccgtgcggg atcatggacc agctcgcggt
    22681 ggccgccggg gtcgagggcc acgctctcct gatcgactgc gcgaccgagg aggtcacgcc
    22741 ggtgccggtc ccctcggcgg cggcgatctg ggtcgtcgac tccggacagg tgcgaacgct
    22801 ggccgactcc gcctatgccg aacgccgggc ccaggccgac gcggcggccg cactcgtcgg
    22861 cccgttgccg cgtgcgtcga tggcatcgat cgcctcggtg gccgacccgg ttctgcgagc
    22921 ccgggcccgt cacgtccgca cagagtgcga ccgggtccac gccgccgccg ccgccctcgt
    22981 cgccggggac ctcgtccggg tcggggagct gatggtcgag tcgcacgtct cgctgcgtga
    23041 cgacttccag gtgtcgacac ccgagctcga cgccctggtc gaggacctgg tgcagacccc
    23101 cggggtgcac ggcgcccggc tcaccggcgc gggcttcggc gggaacgtcg tcgtcctcgc
    23161 cgaggacgac gtgaccgtgc ccggcacgcg tatgcacgcc tcggccggcg cccggaccct
    23221 cgccgcccca ccgaactcgt agcccccggt caccccggcg acctgccagc tacaacgtcg
    23281 gcaggagaac gcggtggacc accggaacgg cgaccgcgaa catcgccccg tgcacgaccg
    23341 aactccggac cccgacctca ccagcctcga ccgcctcggc ccgcggatac ccgcggaggt
    23401 cctcgaggcc tgcaaccgcc tccccaccaa gcccgacccg gtggtcctga tcggcgagcg
    23461 ggtcagggtc cgccccgcga ccctcgacga cgccgtcgaa ctgttcacca tcagcaacgg
    23521 cgaaccggtc acgcgcctcg gtcggtcgac cgaggcctac gacgccgatg aactggtctg
    23581 gcgcttcatg cccgtcgggc cgttccggtc tcccgagacc tacgccgagc acctcggatc
    23641 gatcctcgac cgtcccgact ggcagacctt cgtcgtcgag gacgccgcca ccggcgaggc
    23701 gctcggctcg gccagcctca tcgccaactc gcccacggac ctcaaggtcg agatcggggc
    23761 gctgtggtgc acccccgcgg tccagggcct gggcgtgatc caggaggcgt gcctgctcca
    23821 gatcgagcac ctgttgacgc tcggctacca gcggatcgag tggaagtgcc acgccggcaa
    23881 cgcccggtca cgggccgcgg cggtccgact cgggttccgc ttcgagggga tccaggacca
    23941 ccacatggtc atgaaggggc gtcgccgaga cacggcgtgg ttccggatcc tggccgacga
    24001 gcgggctcag accgacgggg gctgacgcgc caagagctgc gccttctggg cggcgaactc
    24061 ggcgtcggtg aggtggccct ggtcgcgcag cgcggcgagc ttggcgatct cgtcggccac
    24121 cgacccgccg gcgccgggac cctccgccgg agtccggatc cggtcgaact tgcggttctc
    24181 gttgtcctcc atcgtctggt acagcaggtt ctgcaccatc accgggttgc ggatgtcctc
    24241 gaagcgctgc tcgccgccgg tgccagccga ctcgatcatc aggtcgccgg cgcgcaccag
    24301 ccgctcgaac aggtgctggt ggaagaacac cgtgttgatc cgttcgagcg ggatctcgat
    24361 gccgcgcttg gagatgatcc cctcgcggta gatgcagcgg tcggtggtga tgacgaagtt
    24421 ggtcgagtac cacttgatca gccgctgggc caggtacagg aggctgacga ccagagcgag
    24481 gatcgccacc cacttggtgc ccgtgccgaa ccatccggtc cagccctgca cgagcaggaa
    24541 gatcccgaac gcgatcgcca gcaccaccca gaccaccgac cacatcagca tgatccagtg
    24601 ggggtgctcg tcgacgacga tctgctcgtt ggcgtgcagg ttgctgcggt cgtaggccac
    24661 ggccgccagc gtaacggccc gatccgacac cgccgcggga tccgctgtca gcgcgcactg
    24721 gtacgttcgc cgcggtggca cccgagaccg accggctcct gcgcggcatg aacgagcacc
    24781 aggtcgcggc ggtgctgtcg gacgcgtcgc cgctgcgcat cctcgccggt gccggatcgg
    24841 gcaagacccg cgtgctgacc caccgcatcg cccaccggtc cgaggcgggc acgctggatc
    24901 cgtcgcgggt gctggcggtg acgttcaccc gcaaggccgc cggcgagctg cgcgagcgcc
    24961 tcggtcggct cggcttgcgc gacggcgtgc acgccggcac gttccacgcc atcgcctacg
    25021 cccagctccg gcagcgatgg gaggagcgcg gcatcacccc gcccgagctg ctcgaccgca
    25081 agttcgccat cgtggcgcga ctcctgcccg gacggggccg caaggacgac cgcagcacac
    25141 cgctcgacgt cgtcgccgag atcgaatggg ccgccgcgcg ccgcatcacc ccgcaggact
    25201 acgaacgtga ggccacccgg gcccgccgac gtccgccggt gcccctcgga accgtcgcca
    25261 cggtctacga cgacttcacc cagctcaaac gccgacgacg gctggtcgac ttcgacgaca
    25321 tcctccggct ggcggcccgg gacctcgaag ccgacccggt ctacgccgcg gcccggcgct
    25381 ggcggttccg ccacatcttc gtcgacgagt tccaggacgt gaacccgctg cagttccacc
    25441 tgctgcagca gtggctggga cccgactccg acacctgcat cgtcggtgac cccaaccagg
    25501 cgatctacgc ctggaacggc gccgacgccg gctacctgat cgacttcgac cgctacttcc
    25561 ccggcggcag caccgtcacc ctggaggaca actaccgctc gaccccgcag atcctggcgg
    25621 tcgccaacgg cgtcctgcag cagggaccca gcgtggcgat ccggctccgc ccccaccgac
    25681 cggccggagc ggtccccacc gttcgggcac tacccgacga caccgccgaa gcccgcgccg
    25741 tcgcccgcgc ctgccgcgac ggtcaccgac ccggcgtgcc gtggtcggcc caggcggtgc
    25801 tcgtgcgcac caatgcccag accgctctga tcgccgaggc gatgtccgcc gccggcgtcc
    25861 cccaccgggt gcgcggctcg gggaggctcc tcgagcagcc cgaggtcacc gacgcgctgc
    25921 aggtgctgag gcgctccgcg tcgctcgaga tcggtctggc cgacctcgag cacgacctcg
    25981 gcgaccgatc ccggctcacc gaggagcggg ccgccaacgt gaccgaggtc gtccgactcg
    26041 gccgcgagta cctcgccctc gagcccgccg gggacaccgc ggcgttcctg gcctggctct
    26101 ccaccaccct gcgcgacgag gacggtggcg gtggcgacgc cgtcgacatc gtcaccttcc
    26161 acgcggccaa gggcctggag tggccggtag tgcacatcgc cggtctcgag gagggctacg
    26221 tcccgatcca ccacgccgag accgatgctg acgtcgacga ggagcgccgc ctgctctacg
    26281 tggcgctcac ccgagcccgc gacgagctgc actgcacgtg ggcgcgctcg cggacctttg
    26341 ggtcgcgcac catgaatcgc tccccatcga tgtgggtgga ctcgatcgag accaccctcg
    26401 gggtcgctcc caccgagctc tcccgggccc agggcgccga gcgcgcccgc tccgctcgtc
    26461 gctcggtccg gcgggccccg gccgccgagg ggcccgagct cgagctgttc gaggcactgc
    26521 gcagctggcg ccgagaccag gcccgcgccg cggacgtgcc ggcgttcgtc atcttcaacg
    26581 acgcgaccct caccgccatc gcccggcacc gcccgcgcag cgcggcccag ctgctgaagg
    26641 tccccggcat cggcccggtg aaggcccagc ggttcgggcc cgacgtgctg cgcatcgtcg
    26701 ccgacgcgac ctgatcgacg ggcgagcgcg tcgtcgcacg gtgccaggag gccaccctcc
    26761 tcagcgcgag cgttcctcga gcaactgctg gaacgtaccg ggatcgaagg cgatgaacag
    26821 gccctcggcc tcggcccgca gctcaccgtc ggccgtgatc gtggcccggg cccagatctt
    26881 gcggccctcg cgccggtgca gccaaccctc catccgcaac ggcgtgtgca gcggcgtcgg
    26941 ccggcggtag tcgaccccca ggtgggcggt cattccctgg gtgcccgaca acgactgggc
    27001 ggcaccgagc agctcgtcga acgccgcagc cacgtagccg ccgtgcacgc accccggcgg
    27061 cccctcgtag gcggagccga acgtgacgtc gcagacgacc cggtcgccct cgtaggacag
    27121 cgtcatgggc ggcgacatcg ggttggccag tccgatgaac gggctgtggt cgaagaacgc
    27181 ccaccgctcc ggcccgtcgg gcagcgtccc ggcgatcgcc gcttcggcga agccctcgta
    27241 ggtctgcccg gatggcagcc gtcccaacgc cgaggccagg cgctccagat cgtccgcggc
    27301 cgcggcgagc tcgtcggtgt ccgcggtggt ggacaccatc gactcgatga tgtcgcgcat
    27361 ggcctgtgcc aggcggcgga cctcgactcg gcgcggggtc agctcgagcg cggccaggcc
    27421 ctcgatgcgc gggctcgcga agccgccctc ctcggtggcc gtgtccgacg cgacggccgg
    27481 cgccgcatcc gctccgccgg ccgcggcacc gggtccggac ccctcaagat cgtcctgcat
    27541 ccgcgtggcc ctccgatcag gtgccgatgt cgtagaccgc catcaccggg gcgtggtccg
    27601 acggcttgga gcccttgcgg gcgttccggt cgacgaggtc cgcagtggcg cgcccggcca
    27661 gcgacgtcga cgacagcagg tagtcgatcc tcatgccccg acgcttgtgg aagtcgccgt
    27721 tgcggtagtc ccagtacgaa tagatgccgt cctgggggta gcgccgtcgg agggtgtcga
    27781 cgagacccca gtcgaccacg gcgcccaggg ccgcacgttc ctcgacggtc acgtgggtcg
    27841 aaccctcgaa cgcggcgggg ctccagacgt cctggtccgt cggcgcgatg ttccagtcgc
    27901 cgagcagcac gagcgcctcg tcggggctgt ggtgcgcgtc caggtgggct cgcagccgcc
    27961 ccaaccaggc cagcttgtag gcgtagtggt cgtggccgac ctcccggccg ttggggacgt
    28021 agagcgcccc cacgcgcaca ccgccgcagg tggcccacag catccgggcg tcggggtcgg
    28081 gctcgccgcc gtcgtcgaag cccgccacca cgtcgtcgat gcccacccgg gacaggatgg
    28141 ccaccccgtt ccagcgcccc tcgccgtggt gcaccgagtc gtagccgagc cgttggaagt
    28201 ccatgcccgg gaactgggcg tcggacatct tggtctcctg caggcagagc acatcggggc
    28261 ggaccgtggc cagccactcc tccacccggg gcatgcgggc gttcaacgag ttcacgttcc
    28321 acgtggcgat cagcaccgtc ggcaatctac gcgtccgccc ccgtggtcgg gaccgccggg
    28381 cccgacacgg gccacgtccg ggagggcatc ccatcgcccg ggagcgtccg gggcgcgccg
    28441 ataggctgcg gggcgtgaac gactcccccg aacctgccgg aaccgcctcg ccggggacga
    28501 ccgatccggt cgcctccgag ccgctcggcc ccaacgcctg gctggtcgag gagatgttcg
    28561 agcagtacca ggcggacccg ggctcggtca gcgagacgtg gcgggacttc ttcgccgact
    28621 accgcccgcc gggacgtccg gcccccgttg cgacgcccgc gacccctccc tcgaccaccg
    28681 ctccggtcgg gaccgcgcca cccgcaccga ccgcaccacc gtccacagcg ccgtccacat
    28741 cgtcgaccgc cgggccggtc gacgagcccg gcgaccccat ccgcggcgtc ggcgcccgca
    28801 tcgtgtccaa catggaggcc agcctcgagg tcccgaccgc cacgtcgttc cgcgaggtcc
    28861 cggccaagct cctcgagatc aaccgcagcg tcatcaacgg ctacctgggc aggacccggg
    28921 gcggcaaggt cagcttcacc cacatcatcg gctacgccct ggtacgcgcg atcgcggatc
    28981 acgtcccggt gatgaacaac cacttcgtcg aaggctccga cgggacgccc cgcctggttc
    29041 gccgggagca cgtggggctg ggactcgccg tcgacatgga gaagtccgac ggctcccgct
    29101 ccctcgtggt ccccgtcatc aaggacgccg acacgatgga cttcgccggc ttcagcgccg
    29161 tctacgacga gctcatccgc aaggccaaga ccaacaagct caccgtcgac gacttccagg
    29221 gcgccacgat caccctcacc aaccccggca cgatcggcac cgagcggtcg gtcccgcgcc
    29281 tcatgcccgg tcaggggacg atcgtcggcg ccggagcgct gggcttcccc gcggcgtacg
    29341 ccggtgccga cccgcgcacc ctcgccgagc tcggcatgtc caaggtgctc accctcagct
    29401 cgacctacga ccaccggatc atccagggcg ccgagtccgg gctgttcctc aagaaggtcg
    29461 aggagctgct cctgggcgag gacggcttct acgacgacct gttccgctcg atgggcgtgc
    29521 cctacgaggc ggtgcgctgg cgccaggacg cggtgggcga cgaaggacgc gagaccgccc
    29581 aggtggtcaa gcagatgcag gtgtccaccc tgatcaacat gtaccgcgtg cgcggtcacc
    29641 tgatcgcgga cctcgacccg ctcgcggcca aggcccccga gatgcacgcc gagctcgatc
    29701 ccgccaccta cgggctgacc atctgggacc tcgaccgcga gttcctgacc ggcagcgaga
    29761 acggcatcta cgcgccggtc ggcggggtgc cccgtcagcg gctcggggac ctgctccacg
    29821 tgctgcgcga cgcgtactgc cgcacgatcg gcatcgagtt cggccacatc caggagccgg
    29881 cggagaagcg ctggatccag gagcaggtgg agggtcgcag ctcccgggtc gacgtcgagg
    29941 accagcgcca catcctggga cggctcaacg cggccgaggc cctcgagaag ttcctggcca
    30001 ccaagtacct cggccagaag cgcttcggtc tcgaaggtgc cgagtcgctg atcccgatcc
    30061 tcgacgccct gctccaggaa gccgccgacc acggcatgga ctccgcctac ctgggcatgc
    30121 cgcaccgcgg ccgcctcaac gtgctggcca acatcgtcgg caagtcgctc gaccagctgt
    30181 tccgcgagtt cgagggctac gtggaccccg actcgatcca gggctccggc gacgtcaagt
    30241 accacctggg ccaggagggc acgttcgtca gccgcagcgg ggccgagctg ccggtcagcc
    30301 tggccgccaa cccgtcgcac ctcgaggcgg tcgacccggt ggtcgagggc atgacccggg
    30361 cccgcatgga cgagatcccc cacgaccgga cccggcggtt cccggtgctg ccgatcctca
    30421 tgcacggaga cgccgcgttc gccggacagg gcgtcgtggc cgagacgctg aacctcagcc
    30481 tgatcaaggg ctaccgcgtc ggcgggacca tccacgtgat cgtgaacaac cagctcggct
    30541 tcaccacccc tccggagtcc gcacgcagct ccgagtactc caccgacatc gccaaggcgg
    30601 tgcaggcccc gatcatccac gtcaacggcg acgaccccga ggcctgcgtc cgggtggccc
    30661 gcctggcctt cgcctatcgg cagcgcttcc acaaggacgt ggtgatcgac atggtgtgct
    30721 accgccgcca cggccacaac ga
//
LOCUS       DAOQOD010000013        30251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_482_length_30251_cov_11.953934, whole genome shotgun sequence.
ACCESSION   DAOQOD010000013 DAOQOD010000000
VERSION     DAOQOD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 30251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30251
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_30251_cov_11.953934"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..2311)
                     /locus_tag="PLZ72_02805"
     CDS             complement(38..2311)
                     /locus_tag="PLZ72_02805"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNA35259.1"
                     /translation="MAVTASTPIELVEGVYARFEERIDAARERLGRSLTLAEKILIAH
                     LDDPTAAFERGVTYNDLRPDRVAMQDATAQMALLQFMTAGLPEVAVPSTVHCDHLIQA
                     RENGRVDLLAAEEGNAEVYDFLESVSARYGIGFWKPGSGIIHQVVLEQYAFPGGMMVG
                     TDSHTPNAGGLGMIAVGVGGADAVDVMTGFPFNVRWPRLIGVHLTGELSGWSAPKDVI
                     LKVADILTVKGGTGAIVEYFGPGARSLSCTGKATICNMGAEIGATTSLFAYDDAMSRY
                     LKATGREQIAAAADGVAPHLRPDDEVLANPGGFYDEVIEIDLSTLEPHINGPHTPDLA
                     RPVGALGAEATEKGWPLEISAALVGSCTNSSYEDISRAASILRHAADNGLKVKAPLMI
                     TPGSEQVRATIARDGLLEVFEAAGATVLANACGPCIGQWARPAEANDHVNTIVTSYNR
                     NFPKRNDGSANTLAFVTSPETVVALALAGTLDFNPLTGTLTNEAGGQVQLRVPVGEEL
                     PARGFDPGESGFIAPPADRASVEVVVQPTSDRLQLLEPFAAWDGQDYLDLPILLKAQG
                     KCTTDHISMAGPWLKYRGHLENISGNLFLGAVNAFTGVAGEGKDQLDGSTRSFPDIAR
                     HYHEAGQPWVAVGDENWGEGSSREHAAMEPRFRGAKAIIVRSFARIHEANLKKQGVLG
                     LTFDDPATYDQIGEDDRISILGLADLAPGRPVDCLLRKPDGTEIPFRCNHTMSDEHIE
                     WFRAGSALNIIRARTQA"
     gene            2523..3260
                     /locus_tag="PLZ72_02810"
     CDS             2523..3260
                     /locus_tag="PLZ72_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNA35260.1"
                     /translation="MRPIRYRIIAEDLRERITSGEFRLGAVLPSEADLSATYEASRVT
                     VRRALEALRSDGLVESRQGFGWLVSAEPLRQDLTRLATLDGQLGAAGVRSERRIVSFG
                     FRRTPPRVREFLSGRTVLEVVRLHLADDRPFARVTVWCREDLGAALSRDDVERSAFLE
                     QLPVRLGGATQTIGADAASSADAEVLDVPEGAPLLVAERVTRDRSDQVVLVSEHVFPA
                     HLTRFVVELPADDGLLEPAGLRIVDPA"
     gene            complement(3448..3630)
                     /locus_tag="PLZ72_02815"
     CDS             complement(3448..3630)
                     /locus_tag="PLZ72_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35261.1"
                     /translation="MRWYVTLSSWSTRRLERGASLVEYALLLALIALVCVTAVQFLGT
                     SASGSLDRSGSSLFVP"
     gene            complement(3727..3912)
                     /locus_tag="PLZ72_02820"
     CDS             complement(3727..3912)
                     /locus_tag="PLZ72_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNA35262.1"
                     /translation="MMVQFDFLKTWLKAQAKTERGASLVEYALLVALIAVVCIAAVSF
                     IGSSAKSKFSEVGNSLK"
     gene            complement(4060..4707)
                     /locus_tag="PLZ72_02825"
     CDS             complement(4060..4707)
                     /locus_tag="PLZ72_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35263.1"
                     /translation="MAIRLMLADDHRMLREGLRRSMTDAGFDVIGEAGDGVEAVRLAL
                     QLSPDVILMDVTMPNCDGVEACRQVKSTSSDTKVVMLTMHADQDVLTNAIRAGAIGYL
                     TKDCSTREIAEAVRMAAEGDTVLSPQLARSMLEEVRRIDQPRTAEEDRVVTKREEEVL
                     QLIADGCSTPEVAASLFISQKTVKNHLASIYQKLDARDRTQAVLQAVRMGIVSLD"
     gene            complement(4713..6644)
                     /locus_tag="PLZ72_02830"
     CDS             complement(4713..6644)
                     /locus_tag="PLZ72_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNA35264.1"
                     /translation="MHTAPPDDPLSNPPPSDDPSRPRGAFDAPETPTPTPTAEPAEAR
                     RSLVGRFRERLDRDLREALIDPAQQPTGDFDIVAQQLPSMGVDRVASFNSLITAMRLA
                     TTAISLLLVASELNEFGMSVRIWTAVVVGYAIFRAFRPIRYESDLQSLLRVLAEVALH
                     VAAVAMTGYWQSPLIFTLLTAVTIAGLARGFGFSIRIAVVTIVAVSFPYILGAANQRE
                     AFLQSASWGGIVMLVAIIAGYARRISGEAARERELAIDRLSRLSDANALLYSLHRVTQ
                     TLPASLDLGDVLDSTLSRMKSLVAYDSVAVLLFDETDGHWEVVRHQGLHVPTRLGPTE
                     LPPGLRTALTENRPVYVADLQSEGPGLSDRSGSGIYTVLSARGSIIGLLAIEHSDFQH
                     FTSRDLELIEGFVAPASLALDNARWFARLRTVGADEERTRIARDLHDRIGQSLAYLAF
                     SLDRLVERDEAGEGVTEDLARLREDVRGVIREVRDTLYDLRTDVSEEQGLGSVLEQYV
                     GRVGERSHLTIQVEADKDARLPILQEREMWRIAQEALANVERHARATAVRIVWRCDGE
                     RALIDVTDNGVGFEAATAGRLDSYGVLGMRERASSIGASLEILSAPGRGTRVRCSLNP
                     NETGDTRTIPSAVEAGTRR"
     gene            6815..7405
                     /gene="coaE"
                     /locus_tag="PLZ72_02835"
     CDS             6815..7405
                     /gene="coaE"
                     /locus_tag="PLZ72_02835"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228820.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNA35265.1"
                     /translation="MLLIGLTGGIGAGKSSVSAALARRGAVIVDADAIVRELQSPGTD
                     VFGEMVERFGSGIVSEDGTLDRAAVAQVVFTDPQALADLGAIVHPRVHAEIERRIAEQ
                     AGTDHVVVLDIPLLAEAGWPGLAGTIVVDLDPDIAVQRLIAHRGFSEEDARNRIANQI
                     EREQRLSRADFVVDNHGDPEALEAEVDRAWTWIHSI"
     gene            7492..9573
                     /gene="uvrB"
                     /locus_tag="PLZ72_02840"
     CDS             7492..9573
                     /gene="uvrB"
                     /locus_tag="PLZ72_02840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNA35266.1"
                     /translation="MAPVEHTPVIPTRAEPKMVRPFEVVSDFRPAGDQPAAIEALADG
                     VHCGERFQTLLGITGSGKSATIAWTIEQVQRPTLVLAPNKSLAAQLANEFREFFPHNR
                     VEYFVSYYDYYQPEAYMPTTDTFIEKDSSVNDEIERLRHAATSSLLTRRDTIVVASVS
                     CIYGLGSPDEYASRFFTLRVGDVVDQRDLLSQLVHLQYERNDMVLSRGKFRVRGDVIE
                     IQPAYEETAVRIELFGDDVEAIAVVDPLTGEKTAQLDDVLIFAKTHYVVSDERLHAAI
                     GRIETELQERLAFFERSGKLLEAQRLRMRTEYDLEMLQEMGFCNGVENYSAPMEGRGP
                     GDPPNTLIDYFPSDYLTIIDESHQTIPQLHGQYEGDRSRKEVLIEHGFRLPSAADNRP
                     LRFEEWYERAGQTIFLSATPGAWELDHSSRVVEQVVRPTGLIDPEVVIKPTKGQIDDL
                     LVQINDRVSNGERVLVTTLTKKMAEDLTDYLLEMGVKVRYLHSEVDTLQRIELVRDLR
                     LGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSETSLVQMIGRAARNVNGQVH
                     MYADRVTDSMTRAVSETNRRRGLQQAYNAEHGIDPQSVQKAITDILADLRGSDGAPLP
                     GGGRRSRRPGEKARAEQFADLPPQDLGALIQLLEDEMHQASADLRFEEAARLRDEVKE
                     LKRELRNAGGS"
     gene            9727..10689
                     /locus_tag="PLZ72_02845"
     CDS             9727..10689
                     /locus_tag="PLZ72_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNA35267.1"
                     /translation="MRASDLGRLIELASPSAGAFSASQAGEIGVSRRRLLQAHRAGVI
                     NRLHPSVFHVGATISRRARIAGALMAAGPESVASHESALYLHGVERVPFVVAVTTGPT
                     GRTDLQGVRVHRCRDLHPEHIRVVGALDATTIERALVDVATCFSGVRVEWLLDHLTIT
                     TRRTSLGRIARVLRQINHRGRTGIGLFTALLDERAAGPPAPRSLLERSADELLALTGL
                     PAARLEHPLPGADPSVPVGFVDRAWPEVKLLLEIDGRRWHAREADMARDRRRDRQAAA
                     AGWLTLRVLGEEVADVPSAVADEIVAVYRERAVLFDVPIPGVRV"
     gene            complement(10790..11884)
                     /gene="aroC"
                     /locus_tag="PLZ72_02850"
     CDS             complement(10790..11884)
                     /gene="aroC"
                     /locus_tag="PLZ72_02850"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNA35268.1"
                     /translation="MSSSTGTLFRVTTFGESHGGGVGVVVDGCPPRLELSDADIQPDL
                     DRRRPGQSRLVTQRDETDRVEILSGVTDGLTLGSPIAMLVRNQDHRSGAYDHMADIYR
                     PSHADWTTEAKYGIRAVAGGGRASARETIGRVAAGAVARKLLAERVGVEVLAWVSQVH
                     DVVADVDPSSVTVDEVEADPTRCPSTEAASAMTAAIEQARRDGDSLGGVVTCVARGVP
                     AGWGEPVFDKLEADLAKALMSLPASKGFEVGSGFGAASMTGRVHNDPFVPGPDGRPRT
                     TSNHSGGIQGGMSNGEDILLRVAFKPTATISSPQQTVTRAGEAVTLEAKGRHDPCVLP
                     RAVPIVEAAVLMVLADHWLRQAALEGAAPR"
     gene            12150..13826
                     /locus_tag="PLZ72_02855"
     CDS             12150..13826
                     /locus_tag="PLZ72_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35269.1"
                     /translation="MAVLVLVLVLHGPTPVISDDGSVLAQAALLRDGRTGVDLPLAAA
                     DPDGVFPPLENSTTAAGRAFPYSKHLVLPAAVAALTLLVGGAGGVVFSSLSVWLAGLA
                     AMVLAARIDRRLSAVALWSTVLLTPLVFDANLVVATGTAAAALGFLVVAVVSVRARPV
                     TRRLVPVAVLAFVVPMWRTEGMFAVAAVAVLATAEPLVIAARSRRLTGDTVLRVAAGV
                     VAGTAGIAGYVLDVSMAARVVGSTTRIVPSTQDYDPIRGRLSSMWGSLLRPAHDVPAS
                     SGLLVALVAVLVLVAVVLVRRRGPAGRIVTLLVAAAAVSIAWVLTPPALVTGLLPAAP
                     VLFAGLLLLGRADLDVPETRAGLSVSALTALGVVATSYSAGGGAEWGGRYFHILIPLL
                     VPASLVGLSRARDLLSGRDGMVATVAVLVVAVAPSVVALRSVVQLRSGSERTVEVVLD
                     GIDRAERAAEDEPGGPAGDGGDIVVVSARPTFGRFAWNRLDGLRLLSVADPTRLVDAL
                     DGVADTDVDRVVVLAQDDEEPLGDTMGDWTVVERELVGGWQITRMTRSGA"
     gene            13916..16810
                     /gene="uvrA"
                     /locus_tag="PLZ72_02860"
     CDS             13916..16810
                     /gene="uvrA"
                     /locus_tag="PLZ72_02860"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNA35270.1"
                     /translation="MAPPAKAPSPSNTIVIRGAREHNLDDVSLDLPRDRLIVFTGLSG
                     SGKSSLAFDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDVIEGLSPAISIDQKSASR
                     NPRSTVGTVTEIYDYLRLLFARVGVPHDPETGERLVRQTPQQIVDRILQLPEGTRFQV
                     IAPMVRGRKGTYEQLFADVAQRGFTRVRVDGEVHELPADIELARYEQHTIEVIIDRLV
                     LRDGIERRLTDSMETALELAEGMAQIEIVPRRGDDGADAPADAEPELLTFSEKLARPS
                     DGKSFEELAPRNFSFNSPYGACSACDGLGTVFEVDPELVVPDPDQTVAGGAIAPWASG
                     HAKYFHRLLESVTAEYGIDATVPWQKLPKKDQKLLLYGIGDAQRIDVKFKNRYGRTRS
                     YSARYEGIIPYLKRRHSEAESDSAREAVEGYMRQVHCPTCGGARLNPYALAVTVDDLN
                     MHQLCSLSISESRDRLASLHLDDRQALIAERIMKEINTRLQFLCDVGLEYLSLHRSAS
                     TLSGGEAQRIRLASQIGSGLVGVLYVLDEPSIGLHQRDNHKLIETLIRLRDIGNTVLV
                     VEHDEDTIRIADHVVDIGPGAGEHGGRVVHTGTVKGLLRNKESVTGQYLAGKRSIPVP
                     ELRRQPGDEWLSIKGAREHNLRNIDVDIPLGCFVAVTGVSGSGKSTLVNDILLRSLMQ
                     HVYGSKVPPGLHRRVDGMDQLDKVIDIDQSPIGRTPRSNPATYTGVFDHVRKLFAQTP
                     EARIRGYMPGRFSFNVKGGRCEACAGDGTIKIEMHFLPDVYVPCEVCKGKRYNRDTLE
                     ITFKGLNISEVLDLPCEEALEFFSNQPTIARHMQTLVDVGLGYVRLGQPATTLSGGEA
                     QRVKLASELAKRSTGHTIYLLDEPTTGLHFEDIRKLLTVLSRLVDQGNTVLVIEHNLD
                     VIKTADWVIDLGPEGGVGGGQVIVEGPPELIAKTPESFTGQFLAPLIDGA"
     gene            16959..19067
                     /locus_tag="PLZ72_02865"
     CDS             16959..19067
                     /locus_tag="PLZ72_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35271.1"
                     /translation="MGAEGGSPERVAVVEGCRRWWHVVVVAGIALFLAVVVLHPFPLS
                     DPVLYRGDAFQHEILTGAADGWGSPAPSADLAAPDSVDWSVFPTGTERLQLFVLHALR
                     VASGGVIAAVNLYLLVGVVVTAVVAFAVLRWMGLRPLLAGAAAVVFTFAPAFSSAVFQ
                     GHLFLFALYPVALAVWLALWSTMTGPRSRPPRSELVLATSAVVVTSLSSAYYTAFAVV
                     VILAVGSAVAARRRSVSSLLRPGAVVLGLAAVAGASLVPNLIARRGDPAAGALRRTAA
                     DVWRYSLRPQDLLVLGDDHPLGPLARGLRELGSSAVRPGTGTVLGLFALVGCVGCLIV
                     ALRHARAPRDRSDRIVGRLAVVVGAVMAVATVGGIGSWIGELGFTQVRAWSRMVVVLS
                     FVGLAAGGLILQRQISTRSWSAPRVAIGTVVIAALALLDQGFPALDHAAAAQQAGSDA
                     ELVSVIESQVGATARVFELPVVSFPDDPGSGRLLAPAVHSDGHLRFSAGFFRGGESDW
                     QLSWCRQPAGAFVRAVAVAGYDALLIQRNHHLVSDPAALQAQLDAQLGAPTGTSRDGA
                     FVWYDLRPLRAELIDRFGERAVARSGDLVQRPIGVDYAGVTDLRMSGRVMAGGGAVRL
                     RRLDGDGGPVEVTMHVQPDGGEGRTVRSESDLDQVVTEIPVPGPAPTTVSGLSVIDVR
                     ALDDPVLGPRDGDGLPAVCM"
     assembly_gap    19125..19224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19244..20350)
                     /gene="nadA"
                     /locus_tag="PLZ72_02870"
     CDS             complement(19244..20350)
                     /gene="nadA"
                     /locus_tag="PLZ72_02870"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNA35272.1"
                     /translation="MLRLQAPLPEQYTTASPDELAERIAAAKQTLGERLFILGHHYQR
                     DEVMRWADARGDSFRLSVLAKERPEADYIVFCGVHFMAESADILTGDHQKVVLPDLNA
                     GCSMADMADIDSVEEAWESIAEITDIERVLPITYMNSAADLKAFVGEHGGSVCTSTNA
                     RAVLEWALNDPDGPQARQVLFFPDQHLGRNTGHQMGFTEADMRVWNPRRDLGGLDEAD
                     VKDATFLLWKGHCSVHQRFTPANIEAFRAEHPDGVVIVHPECSHEVCVLADEVGSTDF
                     IIRTVDALPAGTVVAVGTEIHLVQRLDAESDKDVRSLDPLICPCSTMFRIDDRHLAWT
                     LENLVEGNVVNQIAVDPDTAHWARVALERMLSIT"
     gene            20723..22600
                     /gene="uvrC"
                     /locus_tag="PLZ72_02875"
     CDS             20723..22600
                     /gene="uvrC"
                     /locus_tag="PLZ72_02875"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNA35273.1"
                     /translation="MMLERPPAGTIPDTPGSYQFKDADGRVIYVGKAKSLRQRLSNYF
                     QNPANLPPRTFQMVSTAESVEWIQVRNEVEALMLEYSLIKQHRPRFNVRLVDDKSYPF
                     LAVTVSDEWPRPMVMRGRRRKGVRYFGPYAHAYAIRDTLDELLRTFPLRTCSDAKFGR
                     HERLGKPCLLFHIEKCAGPCVGEIDAEHYQRLVDELLEFLDGDTEPVLQRLETQMAEA
                     AEALEFEQAARLRDRLATVRRAIERQQMVGDRREDVDVIGIAEDELEASAQVFYVRKG
                     RVVGRFGFLLDKVMDLTAEQTVAAVLEQLYGDEPAVGYPKSVLVPELPEDLDVYQEWL
                     TLQRGSRVDIRVPQRGPKRELAETVTRNATEAFTRHRLKRASDHNSRAKALNELQETL
                     GLPTAPLRIECYDMSHLQGTDYVGSMVVLEDGLPRKQEYRRFKIRGVDGNDDFAAMEE
                     VLTRRLSNYLADRDKPSSEKGRFAYPPQLLLVDGGKGQLGVAVKVLEDLGLSDQIPVA
                     SLAKRFEEVYLPGRSDPVEVRRGSEALFLLQRIRDESHRFAITYHRELRGKRMTRSVL
                     DGIPGLGEKRKQRLIKELGGVRAVQTAELSTLEALPWLPDAVARALHDTLHSPVPPSA
                     P"
     gene            22612..23382
                     /locus_tag="PLZ72_02880"
     CDS             22612..23382
                     /locus_tag="PLZ72_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35274.1"
                     /translation="MPDPTQNPDSGEHPDPWETHAAWWQEQFTDGVDPEYTEQILPLI
                     AAHLPEGADRVTGLAIDVGCGEGQVARVAAAVGLDVLGVDPAQSQIDVAVRRGGGPRY
                     RKGSAVDLPAGDGAADVAIACLVFEHIADLDAALAEVARVLRPGGRFLFLLNHPLLQT
                     PGSGWIDDQVLDPPEQYWRIGPYLREAETIEEVEKDVFIRFFHRPLSRYLNAAHDAGL
                     VLERMEEPAPPPGFLARAEEYADAASVPRLLLLRFTRT"
     gene            complement(23485..24081)
                     /locus_tag="PLZ72_02885"
     CDS             complement(23485..24081)
                     /locus_tag="PLZ72_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNA35275.1"
                     /translation="MRIKLFLPVVAICCAFALFAGACSSTEDSKNKDAEATTTVKAEK
                     ETTTTTAAPTETLAALLVADPQLSEFSGLATEAGLNAMLTDGGPFTVLAPTNAAIDTV
                     PAATMTALEQDPRGALANVLKLHILNGNVTWDDLSEKNGQCVATLGGNVKVTVEGEGD
                     DAAVDFGGAPVPAQDGVKAVNGTILKVDGVTTAPATDC"
     gene            24233..25081
                     /gene="rapZ"
                     /locus_tag="PLZ72_02890"
     CDS             24233..25081
                     /gene="rapZ"
                     /locus_tag="PLZ72_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNA35276.1"
                     /translation="MSEFVVITGVSGAGRTQFGDSLEDLGWFVIDNIPVELIPKVAEL
                     ARFKERGTPVALVVGSGGDLAEIGPALDELRGSGAGVRIVFLDASTPTLVRRYGESKR
                     RHPLAAQAGTVAGAIDLEREVLAEVRQAADIVIDTSELNVHDLRSRVNDLFAESDGSR
                     MQVTLQSFGFKHGVPADVDTVFDCRFLPNPYWIDELRDLTGLDPAVQDYVRSMGLTGE
                     FLRRIEDLFDLLLPAYVAEGKSLLTIGFGCTGGHHRSVTIVETVAEWLRSKGIEPRVR
                     HRDISK"
     gene            25078..25962
                     /gene="yvcK"
                     /locus_tag="PLZ72_02895"
     CDS             25078..25962
                     /gene="yvcK"
                     /locus_tag="PLZ72_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HNA35277.1"
                     /translation="MNADHNGSAPAVVAVGGGHGLATTLRAARRYAGSITAVVTVADD
                     GGSTGRLRAATPRPAPGDLRKCLVALADHDTAIVTAMEHRFSTGELEGHAFGNLLLVA
                     LEESEGDLVGALDEAGRILGCVGRVLPNTTTAVELNARVDSGGVVRGQVAVGERADLR
                     TVSLEPPDAPACTESIEAILSADQVVLGPGSLYTSVLAATAVPGVAEAVRRSSARVVY
                     VCNLHPQVSETAGYGVADHVDALARHGLIPEVVLYDPDTIRGADGVVGARGVRLAAAN
                     GLAHDPVLLGGALAALVA"
     gene            26107..27117
                     /gene="gap"
                     /locus_tag="PLZ72_02900"
     CDS             26107..27117
                     /gene="gap"
                     /locus_tag="PLZ72_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNA35278.1"
                     /translation="MTVRVGINGFGRIGRNFFRAAKARGADIDFVAVNDLGSLDQMAL
                     LLKRDSVAGTYPGTIKEVDGGIDVDGDVLKVVQHRNPAEIPWGDLGVDVVIESTGIFT
                     DRDKAAAHLDGGAPRVIISAPAGGVDATFVVGVNDDTFDPEKHIIVSNASCTTNCFVP
                     LIKVLDDAFGVQKGLMNTIHAYTGDQSLVDGPHKDLRRARAAAVNIVPTSTGAARATS
                     LVLEAMKGRLDGTSLRVPIPTGSITDFTGLLDQDVTVEQVNEAFKAAASEGPLKGILE
                     YTDEPIVSSDIVGSPYSSIFDSGLTMTMGNMVKVLSWYDNESGYSNRLVDLALVVGAA
                     NH"
     gene            27199..28338
                     /locus_tag="PLZ72_02905"
     CDS             27199..28338
                     /locus_tag="PLZ72_02905"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNA35279.1"
                     /translation="MKFGVPELEDLGDVDGRSVLVRADFNVPIRDGVITDDLRIRAAL
                     PTLQWLRERGARVTACTHLGRPKGAPDPAYSVEPVRQRLAELAPGVELLENLRFDPGE
                     TGNDAAFVDSLCEGHDLYVNDAFGASHRAHASIVGPPTRLPSAAGRLLAKEVEVLLGL
                     REQPRRPFTAITGGSKVSDKLGVLTALLDIADDIVIGGGMCFTFFGAMGHPVGSSLFE
                     ADQVDNCARLLDEHGDRLHLPEDVVGLGPGGKIGDPSAGGEVRNFGRSLPDGWMGLDI
                     GPGAAAAFGDVIGESRTVLWNGPMGVFEDPRFEAGTRAVAQAVAECRGFTVIGGGDSA
                     AAIAQFGLADEVDHISTGGGASLELIEQGDLPGLDALRHAPNVAG"
     gene            28380..29159
                     /gene="tpiA"
                     /locus_tag="PLZ72_02910"
     CDS             28380..29159
                     /gene="tpiA"
                     /locus_tag="PLZ72_02910"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNA35280.1"
                     /translation="MASTRKPIISGNWKMHKNHFEAIQAVQKLHYRLDGDDHDNVVVT
                     VHPPFTDLRSIQTIIESDRMKIELGAQNCHWEDQGAFTGEVAPPMLAKLDVAYVIVGH
                     SERRQYFAETDETVNLKVKAVQAAGMIPIMCCGETLEQREAGEAESWVEGQIVAGLAG
                     LGGDKVAALVIAYEPIWAIGTGRTATSEDAQAMCAHIRSVVSATFGPDAAAAVRIQYG
                     GSVKPGNIAELMSQPDIDGALVGGASLEPDEFAALVQYRNH"
     gene            29179..29421
                     /gene="secG"
                     /locus_tag="PLZ72_02915"
     CDS             29179..29421
                     /gene="secG"
                     /locus_tag="PLZ72_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNA35281.1"
                     /translation="MSIIDIVLWVLQFALGIGVIFLVLVHSGRGGGLSDMFGGGLGAS
                     AAGSTVMEKNLDRITVVVGLLFGFNTLLMALVIDPT"
     gene            complement(29465..30145)
                     /locus_tag="PLZ72_02920"
     CDS             complement(29465..30145)
                     /locus_tag="PLZ72_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNA35282.1"
                     /translation="MSTFSDQGPDTDSDTEQGLTARLRDFVDAHDIEWARTVVDDLEA
                     LDHAVYGAVAEASTPTLDSALRRLTKLADNSKLWFGIAAATALVGGRRGRRAALDGVI
                     AIGITSFVVNQPIKRLMPRKRPDREHFAVSVGRQVPLPTSPSFPSGHSASAFAFANAM
                     GAELPWAAFPVHFLAGAVAYSRVHTGVHFPADAMVGALLGAGVGDVVGTVRRHRRERE
                     LRGSVTVP"
BASE COUNT         4348 a        10411 c        10725 g         4667 t
ORIGIN      
        1 gcgggacaaa gctgatcgtg ggggtgggag gggtgggtca ggcctgggtg cgggcccgga
       61 tgatgttgag cgcggacccg gcgcggaacc actcgatgtg ctcgtcggac atcgtgtggt
      121 tgcagcggaa cgggatctcg gtcccgtcgg gcttgcgcag caggcagtcg accgggcgac
      181 cgggggccag atcggccaga ccgaggatgc tgatgcggtc gtcctcgccg atctggtcgt
      241 aggtggccgg gtcgtcgaac gtcagcccga gcacgccctg cttcttgagg ttggcctcgt
      301 ggatgcgggc gaagctgcgc acgatgatcg ccttggcccc ccggaagcgg ggctccatgg
      361 cggcgtgctc gcgggagctc ccctcacccc agttctcgtc gccgacggcc acccacggct
      421 ggcccgcctc gtggtagtgc cgggcgatgt cggggaagga acgggtggat ccgtcgagct
      481 ggtccttgcc ctcgccggcc acgccggtga acgcgttgac cgcaccgagg aacaggttcc
      541 ccgagatgtt ctcgaggtgg ccgcggtact tgagccacgg accggccatc gagatgtggt
      601 cggtggtgca cttgccctgg gccttgagca ggatcggcag gtccaggtag tcctgtccgt
      661 cccacgccgc gaacggttcc agcagctgca gccggtccga ggtcggctgg accaccacct
      721 cgaccgaggc ccggtctgcg ggcggggcga tgaagccgga ctcgccgggg tcgaacccgc
      781 gggcgggcag ctcctcgccg acggggaccc gcagctgcac ctgccccccc gcctcgttgg
      841 tcaacgtgcc ggtgaggggg ttgaagtcga gggtgccggc cagagccagt gcgaccacgg
      901 tctcggggga ggtcacgaac gccagggtgt tggccgagcc gtcgttgcgc ttggggaagt
      961 tccggttgta gctggtgacg atcgtgttga cgtggtcgtt ggcctcggcc ggccgggccc
     1021 actgaccgat gcacggaccg caggcgttgg cgagcacggt cgctccggcg gcctcgaaca
     1081 cctcgagcag cccgtcgcgg gcgatggtgg cgcgcacctg ctcggagccc ggggtgatca
     1141 tcagcggtgc cttgaccttc agtccgttgt cggcggcgtg gcgcaggatc gacgccgccc
     1201 gcgagatgtc ctcgtaggac gagttggtgc aggagccgac gagcgcggcg gagatctcga
     1261 gcggccagcc cttctcggtc gcctctgctc ccagcgcgcc gacgggtcgg gccaggtcgg
     1321 gggtgtgggg cccgttgatg tgcggttcca gggtggacag gtcgatctcg atgacctcgt
     1381 cgtagaaccc gccggggttg gcgaggacct cgtcgtcggg acgcaggtgc ggggccacgc
     1441 cgtcggcggc cgccgcgatc tgctcccggc cggtcgcctt caggtagcgc gacatggcgt
     1501 cgtcgtaggc gaacagcgac gtcgtcgcgc cgatctcggc gcccatgttg cagatcgtcg
     1561 ccttgccggt gcaggacagg gagcgggcgc cgggaccgaa gtactcgacg atggcgccgg
     1621 tgccgccctt gacggtgagg atgtcggcga ccttcaagat gacgtccttg ggtgccgacc
     1681 agccgctgag ctcaccggtg aggtggacgc cgatgaggcg gggccagcgc acgttgaacg
     1741 ggaagccggt catcacgtcg acggcgtcgg cgccgccgac gccgacggcg atcatcccca
     1801 acccgccggc gttgggggtg tggctgtcgg ttccgaccat catcccgccg gggaacgcgt
     1861 actgctcgag cacgacctgg tggatgatgc ccgagccggg cttccagaac ccgatgccgt
     1921 agcgggccga caccgactcg aggaagtcgt agacctcggc gttgccctcc tcggccgcga
     1981 gcaggtcgac ccggccgttc tcccgggcct ggatcaggtg atcgcaatgc acggtggagg
     2041 ggacggcgac ctcggggagt ccggcggtca tgaactgcag gagggccatc tgggcggtgg
     2101 cgtcctgcat ggcgacccgg tccggacgga ggtcgttgta ggtgacgccc cgttcgaagg
     2161 ccgcggtggg gtcgtcgagg tgggcgatca ggatcttctc ggccagggtc agcgagcgac
     2221 cgaggcgttc ccgcgctgcg tcgatgcgct cctcgaagcg ggcgtagacc ccttcgacga
     2281 gctcgatcgg ggtggatgcg gtgacggcca tgtcgctctc ctcggttcgg gtccggcgcg
     2341 gagcgggggt aggactcccg ggggagcggt gctcccgccc ggcgccggac cggtcggctg
     2401 gtgctgctcg aacgagcaat acaagtacaa tacatgttcg tggccgatgt gaccgataca
     2461 ccccggagcc ctcgtggacg gacccggtgt cgccggaccg tcggccggac ggaggggcgc
     2521 acgtgaggcc gatccgctac cgcatcatcg ccgaggacct gagggagcgc atcacgtccg
     2581 gtgagttccg cctgggtgcg gtgctgccgt ccgaggccga cctctccgcc acctacgagg
     2641 ccagccgggt caccgtgcgg cgggcgctcg aggccctgcg ctccgacggc ctggtcgaga
     2701 gccggcaggg tttcggttgg ctggtctccg ccgagccgct ccgacaggac ctgacccgac
     2761 tggccaccct ggacggccag ctcggcgccg ccggggtccg ctcggagcgc cggatcgtca
     2821 gcttcggctt tcggcgcacg ccgccccggg tccgggagtt cctgtccgga cgcaccgtgc
     2881 tcgaggtcgt ccggctccac ctggccgacg accgaccgtt cgcccgcgtc accgtctggt
     2941 gccgcgagga cctcggcgcc gcgctcagcc gggacgacgt cgaacgatcc gcattcctcg
     3001 agcagctgcc ggtccgcctc ggcggcgcca cccagacgat cggcgccgat gcggcgtcgt
     3061 cggccgacgc cgaggtgctc gacgtccccg agggcgcacc gctgctggtg gccgagcggg
     3121 tgacccgtga ccgttccgac caggtcgtgc tggtgagcga gcacgtcttc ccggcgcacc
     3181 tgacccgatt cgtggtcgag ctccccgccg atgacggact gctggagccg gccggactcc
     3241 gaatcgtcga cccggcctga ggtccgcacg aggcgacgcg cccgctggac gcgccgatgg
     3301 acgggccacg tcatcggggc ccgtccatcg ggagaccgga gcggacgcaa cgccggcccc
     3361 gggatggctg tcgggtccac ggtcggggtc gtcccgtgcg tggcccgtgg tgtgggtcgg
     3421 tcccggtcga tcgacgggag ctccccgtca ggggacgaag aggctggatc ccgagcggtc
     3481 gaggctgccg ctggccgagg tgcccaggaa ctgcacggcc gtcacgcaca ccagtgcgat
     3541 cagggcgagc agcagggcgt actcgacgag gctggctccc cgctcgagcc gtctggtcga
     3601 ccaggaggac aaggtgacgt accaccgcat ggcgtcctcc aggagtcgtg gtcgacgatg
     3661 ctcccgggaa cgacggaggg gcgggtctga tcgacccgcc cctcgcgtcc gttcccggtc
     3721 agggcatcac ttgagggagt tgccgacctc gctgaacttg ctcttggcgc tggagccgat
     3781 gaacgagacc gcagcgatgc agaccacggc gatcagggcg accagcaggg cgtactccac
     3841 gagggaggcg ccacgctcgg tcttggcctg ggccttgagc caggtcttga ggaaatcgaa
     3901 ctgaaccatc atgttgggat tctcccgtgt gtgagtgtgc tggtgctggt gctgggtggc
     3961 ggacccgccg aggcgatccc gctcccttga gttatcgacg actgaccccg gcccttgatg
     4021 ggtcattggg accattcgtc gacgggtgga accgcccgct cagtcgagcg acacgatccc
     4081 catccgcacg gcctggagga cggcctgggt acggtctcgg gcgtcgagct tctggtagat
     4141 cgacgccagg tggttcttga cggtcttctg cgagatgaag aggctggcgg ccacctcggg
     4201 ggtggagcag ccgtcagcga tcagctggag gacctcctcc tcgcgcttgg tgacgacccg
     4261 gtcctcctct gcggtacggg gctggtcgat ccgtcggacc tcttcgagca tcgaccgggc
     4321 gagctgcggt gacagcacgg tgtcgccctc cgcggccatc ctcaccgctt cggcgatctc
     4381 tcgggtcgag cagtccttgg tgaggtagcc gatcgccccg gcccggatcg cgttggtgag
     4441 cacgtcctga tcggcgtgca tggtcagcat cacgaccttg gtgtccgacg atgtgctctt
     4501 gacctgccgg cacgcctcga cgccgtcgca gttcggcatg gtgacgtcca tgaggatgac
     4561 gtcggggctc agctgcaggg cgagtcggac ggcctcgacg ccgtcgccgg cctcgccgat
     4621 cacgtcgaag ccggcgtcgg tcatcgatcg gcgcaggccc tctcggagca tgcggtggtc
     4681 gtcggccagc atcaggcgga tggccatggg ggtcacctcc gggttccggc ctcgacggcc
     4741 gaggggatcg tccgggtgtc cccggtctcg ttggggttga gggagcagcg gacccgggtg
     4801 ccgcgacccg gagcgctgag gatctcgagc gacgcgccga tgctcgaggc gcgctcgcgc
     4861 atgccgagca cgccgtacga gtcgagccgg cccgcggtgg cggcctcgaa gccgacgccg
     4921 ttgtcggtca cgtcgatgag ggcacgctcg ccgtcgcaac gccacacgat ccgcacagcg
     4981 gtggctcggg cgtggcgctc gacgttggca agtgcctcct gggcgatgcg ccacatctcg
     5041 cgctcctgga ggatcggcag acgggcgtcc ttgtcggcct cgacctggat cgtcaggtgg
     5101 ctgcgctcgc cgacgcggcc gacgtactgc tcgagcaccg accccaggcc ctgctcctcg
     5161 gacacatccg tgcggaggtc gtagagcgtg tcgcgcacct cgcggatgac gccgcggacg
     5221 tcctcgcgca ggcgggccag gtcctcggtg acgccctcgc cggcctcgtc ccgctccacc
     5281 agtcggtcga gcgagaacgc caggtacgcg agcgactggc cgatacggtc gtgcaggtct
     5341 cgggcgatgc gggtccgctc ctcgtccgcc ccgaccgtgc gcagccgggc gaaccaccgg
     5401 gcgttgtcga gggcgagcga cgccggggcg acgaacccct cgatcagctc aaggtcgcgg
     5461 gaggtgaagt gctggaagtc gctgtgctcg atggcgagca ggccgatgat cgacccgcgg
     5521 gccgacagga cggtgtagat gcccgagccg gaccggtccg acagcccggg gccctccgac
     5581 tgcaggtcgg cgacgtagac gggccggttc tcggtcagcg ccgtgcgcag gccggggggg
     5641 agctcggtcg ggcccaggcg ggtggggacg tgcagtccct ggtggcggac gacctcccag
     5701 tggccgtcgg tctcgtcgaa gagcagcacg gccacgctgt cgtaggcgac cagcgacttc
     5761 atccggctga gggtcgagtc gagcacgtcg cccaggtcga gcgacgcggg caacgtctgg
     5821 gtgacgcggt gcagcgagta cagaagcgcg ttcgcgtcgc tcagtcggct cagtcggtcg
     5881 atcgccagct cgcgctcccg ggccgcctcg ccggagatgc ggcgggcgta gccggcgatg
     5941 atggcgacga gcatgacgat cccgccccac gacgccgact gcaggaatgc ttcgcgctgg
     6001 ttggcggcgc caaggatgta cgggaagctg accgcaacga tcgtgaccac cgcgatgcgg
     6061 atggagaacc cgaagccgcg ggccaggccc gcgatcgtga ctgcggtcag cagggtgaag
     6121 atcagcggcg actgccagta gccggtcatg gcgaccgcgg cgacgtgcag ggccacctcg
     6181 gccagcaccc gcagcagcga ctggaggtcg ctctcgtagc ggatcgggcg gaacgcccgg
     6241 aagatggcgt acccgacgac gacggcggtc cagatccgca ccgacatccc gaactcgttg
     6301 agctcgctgg cgaccagcag cagggagatc gccgtggtgg ccagccgcat cgccgtgatg
     6361 aggctgttga agctggcgac ccggtccacc cccatgctcg ggagctgctg ggcgacgatg
     6421 tcgaagtcgc cggtcggctg ctgggccggg tcgatcagcg cttcgcgcag gtcgcggtcc
     6481 aggcgctcgc ggaaacgccc gaccaggctg cggcgtgcct ccgccggctc ggcggtcggg
     6541 gtcggcgtgg gggtctcggg cgcgtcgaag gccccccggg gtcgcgacgg gtcgtcggac
     6601 ggtggagggt tggagagcgg atcgtccggc ggcgcggtgt gcatggagga tcgtcccgag
     6661 gtcgtgggtc ggtgcggacg caccggtggc tgtggtggtc catccatcgg caggctcggt
     6721 ccgggacttt gggaaattcc ccagaacctg cgattcggca tgtcggggcg acgggtgggc
     6781 cgggcgccct ccacctccgg tagcgtcggt cgccgtgctg ctgatcggac tcaccggtgg
     6841 gatcggagcg gggaagtcgt cggtgtccgc ggccctggcg aggcgaggcg cggtgatcgt
     6901 cgacgccgac gccatcgtgc gggagctgca gtcgccgggc accgacgtct tcggcgagat
     6961 ggtggagcgg ttcggctcgg ggatcgtgtc ggaggacggg accctggacc gcgccgcggt
     7021 cgcacaggtg gtcttcaccg acccgcaggc gctcgcggat ctgggggcga tcgtccaccc
     7081 ccgggtgcac gccgagatcg agcggcgcat cgccgaacag gccggcaccg accacgtcgt
     7141 cgtgctcgac atcccgctgc tggccgaggc gggctggccc ggtctcgccg gcacgatcgt
     7201 ggtcgacctc gaccccgaca tcgcggtgca gcgcctgatc gcccaccggg ggttctccga
     7261 ggaggacgcc cgcaaccgca tcgccaacca gatcgagcgc gagcagcgcc tgagccgggc
     7321 ggacttcgtg gtcgacaacc acggcgaccc cgaggccctc gaggccgagg tcgaccgcgc
     7381 ctggacctgg atccactcga tataggggtc ctcaacgaca caccttgtcg ctttcccgac
     7441 ctcagaaatc ggcggcggcg gccgtcggac ccccgacgta gcctcctggt cgtggccccc
     7501 gtcgaacaca ccccggtgat cccgacccga gcagaaccca agatggtgcg gccgttcgag
     7561 gtcgtcagcg acttccgccc ggccggggac cagcccgctg ccatcgaggc gctggccgac
     7621 ggcgtgcact gcggggagcg gttccagacg ctgctcggca tcaccgggtc cggcaagtcc
     7681 gccacgatcg cctggacgat cgaacaggtg cagcgcccca ccctggtgct ggcgcccaac
     7741 aagagcctgg ccgcccagct cgccaacgag ttccgcgagt tcttccccca caaccgggtc
     7801 gagtacttcg tctcctacta cgactactac cagcccgagg cgtacatgcc gaccacggac
     7861 accttcatcg agaaggacag ctccgtgaac gacgagatcg aacggctgcg ccacgccgcc
     7921 acctcgtcgc tgctgacccg ccgcgacacg atcgtggtgg cgtcggtcag ctgcatctac
     7981 ggcctggggt cacccgacga gtacgcatcg cggttcttca ccctgcgcgt cggcgacgtg
     8041 gtcgaccagc gagaccttct gagccagctg gtccacctcc agtacgagcg caacgacatg
     8101 gtcctcagcc gcggcaagtt ccgggtgcgg ggcgacgtga tcgagatcca gcccgcctac
     8161 gaggagacgg cggtgcgcat cgagctcttc ggcgacgatg tcgaagccat agcggtggtc
     8221 gacccgctga ccggcgagaa gaccgcccag ctcgacgacg tgctgatctt cgccaagacc
     8281 cactatgtgg tgtccgacga gcgcctgcac gccgccatcg ggcgcatcga gaccgagctg
     8341 caggagcgcc tggccttctt cgagcgctcc ggcaagctgc tcgaggcaca gcggctgcgc
     8401 atgcgcaccg agtacgacct cgagatgctg caggagatgg ggttctgcaa cggcgtcgag
     8461 aactactccg cccccatgga gggccgcggt ccgggcgatc cgcccaacac cctcatcgac
     8521 tacttcccat ccgactacct gacgatcatc gacgaatccc accagaccat cccccagctg
     8581 cacggccagt acgaaggcga ccgatcccgc aaggaggtcc tgatcgagca cggcttccgt
     8641 ctcccgtccg cggccgacaa caggccgctg cgcttcgagg agtggtacga gcgggccggt
     8701 cagaccatct tcctgtcggc cacaccaggg gcatgggagc tcgatcactc gtcgcgggtc
     8761 gtcgaacagg tcgtgcgacc gaccgggctg atcgaccccg aggtcgtgat caaacccacc
     8821 aagggccaga tcgacgacct gctggtccag atcaacgacc gggtcagcaa cggcgagcgg
     8881 gtgctggtca ccacgctgac caagaagatg gccgaggacc tgaccgacta cctgctcgag
     8941 atgggcgtca aggtccgcta cctgcactcc gaggtcgaca ccctccagcg catcgagctg
     9001 gtccgcgacc tgcgcctggg cgagttcgac gtcctggtcg gcatcaacct gctccgggag
     9061 gggctcgaca tccccgaggt ctcgctggtg gccatcctcg acgccgacaa ggagggcttc
     9121 ctccgttccg agacgtcgct ggtgcagatg atcggccgag cggcgcgcaa cgtgaacggc
     9181 caggtccaca tgtacgcgga ccgggtcacc gactcgatga cccgagccgt gtcggagacc
     9241 aaccgacggc gagggctgca gcaggcgtac aacgccgagc acgggatcga cccgcagtcg
     9301 gtgcagaagg cgatcaccga catcctcgcc gaccttcgtg gttccgacgg agcgccgctg
     9361 cccggcggcg gtcgccgcag ccgacgtccg ggggagaagg cccgagccga gcagttcgcc
     9421 gacctcccac cccaggacct gggcgccctg atccagcttc tcgaggacga gatgcaccag
     9481 gcctcggccg acctgcgctt cgaggaggcg gcccgactgc gtgacgaggt caaggaactc
     9541 aagcgcgagc tgcgcaacgc cggcggctcc tgaaggactg gtcggtcgcg tcgggcttcg
     9601 cgtgtggatc gggcgcgtag cgtgtcgttc tcgcacgcgg aggctgagct accactgctg
     9661 caggcctgca tcaacgggtc gcggtccccg ttgccggcac cttgcgcgac gtgatcccgt
     9721 cggtgtgtgc gagcttcaga cctcggtcgt ctcatcgaat tggccagccc cagtgccgga
     9781 gcgttcagcg cgtcgcaggc gggggagatc ggcgtcagcc gtcggcggct gctgcaggcc
     9841 catcgggccg gcgtgatcaa ccggctccat ccatcggtgt tccacgtggg cgcgaccatt
     9901 tcccgccgag cgaggatcgc cggggcgctg atggcagcgg gccccgagtc ggttgccagt
     9961 cacgagtcgg cgctctacct gcacggggtc gagcgggtgc ccttcgtggt ggccgtgacg
    10021 acgggaccga ccggccggac cgacctgcaa ggtgtgcggg tgcaccgctg ccgggatctc
    10081 caccccgagc acatccgcgt ggtcggtgct ctggacgcca ccacgatcga acgtgcgctg
    10141 gtcgacgtgg cgacctgctt ctccggcgtt cgggtggagt ggctgctgga ccacctgacg
    10201 atcaccaccc gtcgcacgtc gctcgggcgg atcgccaggg tgctgcgcca gatcaaccat
    10261 cggggtcgca ccggcatcgg cttgttcacc gctctgctcg atgaacgggc cgcgggcccg
    10321 cccgctccgc ggagcttgct cgagcgctct gccgacgagc tgctcgcctt gaccgggctt
    10381 ccggccgcac ggctggagca cccgctgccc ggtgccgatc cgtcggtgcc ggtgggcttc
    10441 gtcgaccggg cgtggcccga agtgaagctc ctgttggaga tcgacgggcg cagatggcat
    10501 gcgcgtgagg cggacatggc ccgggatcgt cgacgggacc ggcaggcggc agcggctggt
    10561 tggctgacgt tgcgcgtcct gggcgaggag gtggcagatg tgcccagcgc tgtcgcggac
    10621 gagatcgtcg ccgtgtaccg ggagcgtgcg gtgctcttcg acgtgccgat cccaggtgtt
    10681 cgcgtgtgag gtcgacgcgt ggcgggtcgt tctcgcacgc ggagggtggg tgccggtgtt
    10741 cgcgtgtgag gtcgacgcgt ggcgggtcgt tctcgcacgc ggagggtggt cagcgcgggg
    10801 cggcgccttc gagggcggcc tggcggagcc agtggtcggc caggaccatc agcacggcag
    10861 cctcgacgat cggaaccgcc cggggcagca cgcacgggtc gtgtcgaccc ttggcctcca
    10921 gcgtcaccgc ctcgccggca cgggtgacgg tctgctgggg ggaggagatc gtggcggtgg
    10981 gcttgaacgc cacgcgcagg aggatgtcct cgccgttcga catcccgccc tggatgccgc
    11041 cggagtggtt ggaggtggtg cgcgggcgcc cgtcgggtcc gggcacgaac gggtcgttgt
    11101 ggacccggcc ggtcatcgac gccgcaccga agccggaacc gacctcgaac cccttcgatg
    11161 ccggaaggct catgagcgcc ttggccaggt cggcctcgag cttgtcgaac accggctcgc
    11221 cccatcctgc gggcactcct cgggccacgc aggtcaccac cccgccgagc gagtcgccgt
    11281 cacggcgggc ctgctcgatg gcagcggtca tggccgacgc cgcttcggtc gacgggcagc
    11341 gggtcgggtc ggcttcgacc tcgtcgacgg tcaccgacga agggtcgacg tcggccacca
    11401 cgtcgtggac ctgtgacacc caggccagca cctccacgcc gacccgctca gccagcagct
    11461 tgcgggcgac cgccccggcg gccaccctgc cgatcgtctc gcgggccgac gcccgccccc
    11521 cgccggccac agcgcggatc ccgtacttgg cctcggtggt ccagtcggcg tgggacggcc
    11581 ggtagatgtc ggccatgtgg tcgtaggcgc cggagcggtg gtcctggttc cgcacgagca
    11641 tggcgatggg ggagcccagc gtgagcccgt cggtcacccc ggagaggatc tcgacccgat
    11701 cggtctcgtc acgctgggtg accaggcggc tctgtccggg gcggcgtcgg tccagatcgg
    11761 gctggatgtc ggcatcggag agctcgagcc gcggcgggca gccgtcgacg accacgccga
    11821 caccgccgcc gtgggactcg ccgaaggtcg tgacgcggaa cagcgtcccg gtgctcgagg
    11881 acatggggtc agtatggcag cgcgctcccc aggcgctgtc gtccgggacc gttcagttcg
    11941 gaccgggatc ggccgacgac aggacggtga ccctgctcga cccgtcgcgt cccggagccg
    12001 gatcggccgg ccaggacgtt ccaccggcct cggactccgt gccgggcccg ggatccgcgg
    12061 gcccgacgcg gtcggtgcgc cgcggcgtgg cgacagccgc aaccggtccc tcggacctac
    12121 ggctcgcgct gcatgccgca ggggcgctgg tggcggtcct ggtcctggtt ctggtcctgc
    12181 acggaccgac accggtcatc tccgacgacg ggtcggtcct ggcccaggcg gcccttctgc
    12241 gagacggtcg caccggggtc gacctcccgc tggctgcggc ggaccccgac ggcgtgttcc
    12301 cacctctcga gaacagcacg acggcggccg gccgggcctt cccgtacagc aagcacctgg
    12361 tcctgcccgc ggcggtcgca gccctcaccc tgctcgtcgg cggggccggc ggcgtcgtgt
    12421 tctcatctct gagcgtctgg ctggcgggtc tcgccgcaat ggtgctcgcg gcacgaatcg
    12481 atcgtcggct ctctgcggtc gccctctggt caacggtgct gctcacaccc ctcgtcttcg
    12541 acgccaacct ggtcgtcgca accggcacgg cggccgcggc cctcggtttc ctggtcgtgg
    12601 cggtcgtctc ggtccgcgct cgaccggtga cgcggcgcct cgtccccgtg gcggtgctcg
    12661 ccttcgtcgt tccgatgtgg cgcaccgagg gcatgttcgc ggtggcggcc gtagccgtcc
    12721 tggccacggc agagccgttg gtgatcgccg cccgctcccg ccggctcacc ggcgacaccg
    12781 tcctgcgagt ggctgccggg gtggtggcgg ggaccgcggg gatcgccggc tacgtgctcg
    12841 acgtctccat ggctgctcgc gtcgtgggtt cgaccacccg gatcgtgccg tccacccagg
    12901 actacgaccc gatccgtggg cgcctcagct caatgtgggg atcgctgctc cggcccgccc
    12961 acgacgtacc cgcctcctca ggactgctgg tcgccctggt cgcggtgctg gtgctggtcg
    13021 ctgtggtact ggtgcggcgg cgcgggccag cgggtcggat cgtgacgctc ctcgtcgccg
    13081 cggccgccgt gtcgatcgcg tgggtcctca cccctcccgc cttggtcacc ggtctgctgc
    13141 cggccgctcc ggtgctgttc gcgggcctgc tcctgttggg ccgcgcagat ctggatgtgc
    13201 ccgagacgcg ggccggcctg tcggtgagcg ctctcaccgc gctcggggtg gttgcgacca
    13261 gctactccgc cgggggcggt gccgagtggg gcggccgcta cttccacatc ctgatcccac
    13321 tgctcgtccc cgcatcgctc gtcgggttga gccgagcccg tgacctgttg tcgggtcggg
    13381 acgggatggt ggccaccgtg gccgtcctcg tcgtcgccgt ggcgccgtcg gtcgtcgcgc
    13441 ttcgctcggt cgtccagctc cgctccggat cggagcggac ggtggaggtc gtcctcgacg
    13501 ggatcgacag ggcggaacgg gcggccgagg acgaaccggg cggcccggcc ggagacggcg
    13561 gcgacatcgt cgtggtcagc gccaggccga ccttcgggag attcgcatgg aaccgactcg
    13621 atggcctccg gctgctcagc gtggcggacc cgacccggct cgtcgacgcc ctggacggcg
    13681 tggccgacac cgacgtcgat cgggtcgtgg tgctggccca ggacgacgag gagccgctcg
    13741 gcgacaccat gggggattgg acggtcgtgg agcgggagct ggtcgggggc tggcagatca
    13801 cccgaatgac ccgcagtggg gcctgaccga cccacccaca gcacctccag gcggagggtt
    13861 ggagcggcga cgatcacctg acgaacacct gttcgggagg taggctgcgg ggtcgatggc
    13921 acccccggcc aaagccccgt ccccgtcgaa cacgatcgtc atccgcggcg ctcgcgagca
    13981 caacctcgac gacgtctcgc tcgacctccc gcgcgatcgg ctcatcgtct tcacgggcct
    14041 gtcgggatcg gggaagtcca gcctggcgtt cgacacgatc tacgccgagg gccagcgtcg
    14101 ctacgtcgag tcgctgtcgg cgtacgcccg gcagttcctc ggtcagatgg acaagcccga
    14161 cgtcgacgtg atcgagggcc tgtcgcctgc catctcgatc gaccagaagt ccgcgtcgcg
    14221 caacccgcgg tccaccgtcg gcaccgtgac cgagatctac gactacctgc ggctcctgtt
    14281 cgcccgggtc ggcgtccccc acgatcccga gacgggcgag cgcttggtgc gccagacgcc
    14341 gcaacagatc gtcgatcgga tcctgcagct gccggagggg acccggttcc aggtgatcgc
    14401 cccgatggtg cggggtcgca agggaaccta cgagcagctg ttcgcggatg tcgcccagcg
    14461 gggcttcacc cgcgtgcggg tcgacggtga ggtccacgag ctgccggcag acatcgagct
    14521 cgcccgctac gagcagcaca cgatcgaggt catcatcgac cggttggtcc ttcgcgacgg
    14581 catcgaacgc cgcctgaccg actcgatgga gacggcgctc gagctggcag agggcatggc
    14641 ccagatcgag atcgtgccgc gccgaggtga cgacggggcc gacgctccgg cggacgcaga
    14701 gcccgagctg ctcaccttct ccgagaagct cgcccgaccc tccgacggca agtccttcga
    14761 ggagctcgcc ccccgcaact tctcgttcaa ctcgccgtac ggcgcctgca gcgcgtgcga
    14821 cggtctgggc acggtgttcg aggtcgaccc cgaactggtc gtccccgacc ccgaccagac
    14881 ggtggccggc ggtgcgatcg cgccgtgggc gtcggggcac gccaagtact tccaccgcct
    14941 gctggagtcg gtgaccgccg agtacggcat cgacgcgacc gtcccgtggc agaagctgcc
    15001 caagaaggac cagaagctcc tcctctacgg catcggcgac gcccagcgca tcgacgtcaa
    15061 gttcaagaac cgctacggcc gcacccgcag ctacagcgcc cgctacgagg gcatcatccc
    15121 gtacctgaag cggcgccact ccgaggccga gagcgactca gcccgagagg cggtcgaggg
    15181 ctacatgcgt caggtgcact gcccgacatg cggaggggcc cggctcaacc cgtacgcgct
    15241 ggccgtcacc gtcgacgacc tcaacatgca ccagctgtgc agcctcagca tctccgagtc
    15301 ccgggaccgg ctcgccagcc tccacctcga cgaccgccag gccctcatcg ccgagcggat
    15361 catgaaggag atcaacaccc gcctccagtt cctgtgcgac gtcggactcg agtacctgtc
    15421 gctgcaccgc tcggcgtcca ctctgtcggg cggtgaggcc cagcgcatcc ggctggcttc
    15481 acagatcggc tccggtctgg tcggcgtgct gtacgtgctc gacgaaccgt cgatcgggct
    15541 gcaccaacgg gacaaccaca agctgatcga gacgctgatc cggctccgcg acatcggcaa
    15601 caccgtgctg gtggtcgaac acgacgagga cacgatccgc atcgcggacc acgtcgtcga
    15661 catcggcccg ggtgccggcg agcacggcgg ccgggtggtg cacaccggca ccgtcaaggg
    15721 gctcctgcgc aacaaggagt cggtcaccgg ccagtacctg gccggcaagc ggtccatccc
    15781 ggtgcccgag ctgcgccgcc agccgggcga cgagtggctg tcgatcaagg gcgcccgcga
    15841 gcacaacctc cgcaacatcg acgtcgacat ccccctcggc tgcttcgtgg cggtcaccgg
    15901 cgtgtcgggc tcgggcaagt cgacgctggt gaacgacatc ctgctgcgct cgctcatgca
    15961 gcacgtgtac ggctccaagg tcccgcccgg actgcaccgc cgagtcgatg gcatggacca
    16021 actcgacaag gtgatcgaca tcgaccagtc gccgatcggt cgcacgccgc gctccaaccc
    16081 cgccacctac accggcgtct tcgaccacgt ccgcaagctg ttcgcgcaga cgcccgaggc
    16141 gcgcatccgc ggctacatgc ccggccggtt cagcttcaac gtcaaggggg gccgctgcga
    16201 ggcgtgcgcg ggcgacggca cgatcaagat cgagatgcac ttcctccccg acgtgtacgt
    16261 gccgtgcgag gtgtgcaagg gcaagcgcta caaccgcgac accctcgaga tcaccttcaa
    16321 ggggctcaac atctccgagg tgctcgacct tccgtgcgag gaggcactcg agttcttctc
    16381 caaccagccg accatcgccc ggcacatgca gacccttgtc gacgtcgggc tcggctacgt
    16441 ccggctcggc caacccgcga ccacgctgtc gggcggagag gcccagcggg tcaagctcgc
    16501 atccgagctc gccaagcgtt cgaccggcca cacgatctac ctgctcgacg agccgaccac
    16561 cgggctgcac ttcgaggaca tccgcaagct gctgacagtg ctgagtcgtc tggtcgacca
    16621 gggcaacacg gtgctggtca tcgagcacaa cctcgatgtg atcaagaccg cggactgggt
    16681 catcgacctc ggccccgaag gcggcgtcgg cggagggcag gtgatcgtcg agggcccacc
    16741 cgagctcatc gccaagactc ccgagagctt caccggccag ttcctggcgc cgctgatcga
    16801 cggggcctga tctcgccggt cgcggtccgg cggccctgat cgcagcccac cccggagcct
    16861 cggtcgcttc ggcgtgcggt gctcctcccg ctcaacgacg gagcggtcgc tgccgatggg
    16921 ggaccgtgag cgcgacgctg agcagatcta cgcgcccggt gggagcagag gggggctcgc
    16981 ccgagcgagt cgccgtcgtc gagggctgcc ggcgctggtg gcacgtcgtc gtggtcgcgg
    17041 ggatcgccct gttccttgcg gtcgtggtac tgcatccatt ccccctcagc gacccggtgc
    17101 tctaccgagg cgacgcgttc cagcacgaga tcctgaccgg cgctgctgac ggatggggca
    17161 gccccgcgcc gagtgcggat ctcgccgccc cggactcggt cgactggtcg gtcttcccca
    17221 ccgggaccga gcgactccag ctgttcgtgc tccacgcgtt gcgggtggca tccggcgggg
    17281 tgatcgctgc ggtcaacctc tacctcctgg tgggcgtcgt cgtcaccgcc gtggtcgcct
    17341 tcgcggtgct gcgctggatg ggactgcggc cgttgctggc gggagccgcc gcggtggtct
    17401 tcacgttcgc gccggcgttc agctcggcgg tgttccaggg ccacctgttc ctgttcgccc
    17461 tgtacccggt cgcgctggcg gtgtggctcg ccctgtggtc gacgatgacc ggtccccggt
    17521 cgagacctcc acgatcagag ctggtcctgg cgacatcggc ggtcgtggtg acctcgctgt
    17581 cgagcgccta ctacaccgcg ttcgcggtcg tggtcatcct cgccgtcggc tcagcggtcg
    17641 cggcccgacg acgttcggtg agttcactgc tgcggcccgg tgccgtcgtt ctcgggctcg
    17701 ccgccgtggc cggggcatcc ctggttccga acctgatcgc ccgccgagga gatcccgccg
    17761 cgggggcgct gcgacggacg gccgcggacg tgtggcgcta ctcgctgcga ccccaggacc
    17821 tgttggttct cggcgacgac catcccctgg gaccgctggc ccgaggactg cgggagctcg
    17881 ggtcgagcgc cgttcgaccg ggcacgggca ccgtcctggg actcttcgcc ttggtcggat
    17941 gcgtcgggtg cctgatcgtg gcgctccggc acgcccgcgc gccccgggat cgatcggacc
    18001 ggatcgtcgg acgactcgcc gtggtcgtgg gggcggtgat ggccgtcgcg accgtgggag
    18061 gcatcgggtc ctggatcggc gagctcggat tcacccaggt ccgggcgtgg tcgcgcatgg
    18121 tcgtcgtcct ctcgttcgtc ggcctcgccg caggcgggct gatcctgcag cgccagatct
    18181 cgacgcggtc gtggagcgct ccacgagtgg cgatcggcac ggtcgtgatc gcggccttgg
    18241 ccctcctcga ccaggggttc cccgcgctgg accacgccgc cgcggcccag caggccggct
    18301 cggatgccga gctcgtgtcc gtgatcgagt cgcaggttgg tgcgaccgcc cgggtcttcg
    18361 agctcccggt cgtcagcttc cccgacgatc cgggctccgg gcggttgctg gctcccgcgg
    18421 tccactccga tggacatctg cgcttcagtg ccgggttctt ccgaggtggg gagtcggact
    18481 ggcagctcag ctggtgccgg cagccggccg gagcgttcgt gcgtgcggtt gccgtggcgg
    18541 gatacgacgc cctgctcatc cagcggaacc accacctcgt gtccgaccca gcggctctac
    18601 aggcccagtt ggatgcccag ctcggcgctc ccaccggcac gagccgggac ggggccttcg
    18661 tctggtacga cctccggccg ctgcgggccg agctgatcga ccgcttcggt gagcgggcgg
    18721 tcgctcgctc cggagacctg gtgcagcggc cgatcggggt cgactacgcg ggcgtcaccg
    18781 acctgcgaat gagcggacgg gtgatggccg gcgggggagc ggtccgcctc cggcgcctcg
    18841 atggcgacgg tggcccggtc gaggtgacga tgcacgtcca gcccgatggt ggggaggggc
    18901 ggactgtgcg ctccgaatcg gacctcgacc aggtggtcac cgagatcccc gttccggggc
    18961 cggccccgac gacggtgtcg ggtctgtcag tgatcgacgt gcgcgccctg gatgacccgg
    19021 tcctcggacc ccgtgacggt gacggcctcc ccgccgtctg catgtgacgg cgggcggcgc
    19081 tggagggccc ctcaggtgat cgacagcatg cgctccagcg ccacnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnggcggc gctggagggc ccctcaggtg atcgacagca
    19261 tgcgctccag cgccacgcga gcccagtggg cggtgtcggg gtcgacggcg atctggttga
    19321 ccacgttgcc ctcgaccagg ttctcgaggg tccaggccag atgccggtcg tcgatgcgga
    19381 acatcgtcga gcacgggcag atcagcgggt cgagcgaccg gacgtccttg tcggactcgg
    19441 cgtcgaggcg ctgcaccagg tggatctcgg ttccgaccgc gacgacggtg cccgcgggca
    19501 gggcgtcgac ggtgcggatg atgaagtcgg tggagccgac ctcgtccgcg agcacgcaca
    19561 cctcgtggct gcactccggg tgcacgatca ccacgccgtc gggatgctcc gctcggaacg
    19621 cctcgatgtt ggccggcgtg aagcgctggt gcaccgagca gtggcccttc cacaacagga
    19681 acgtggcgtc cttcacatcg gcctcgtcga ggccgcccag gtcacgcctc gggttccaga
    19741 cccgcatgtc ggcctcggtg aaccccatct ggtgaccggt gttgcggccc aggtgctggt
    19801 ccgggaagaa cagaacctgg cgggcctgcg gtccgtcggg gtcgttgagc gcccactcga
    19861 ggacggcccg ggcgttggtc gacgtgcaca ccgacccccc gtgctcgccg acgaacgcct
    19921 tcaggtccgc ggcggagttc atgtaggtga tcggcaggac ccgctcgatg tcggtgatct
    19981 cggcgatcga ctcccatgcc tcctcgaccg agtcgatgtc ggccatgtcg gccatcgagc
    20041 agccggcgtt gaggtcgggg agcacgacct tctggtggtc gccggtcagg atgtcggccg
    20101 actcggccat gaagtgcacg ccgcagaaca cgatgtagtc cgcctcgggg cgctccttgg
    20161 cgagcaccga gagacggaac gagtcgccgc gggcgtcggc ccagcgcatg acctcgtcgc
    20221 gctggtagtg gtggccgagg atgaacagcc gttcacccag cgtctgcttg gccgcggcga
    20281 tgcgttcggc caactcgtcg ggggacgccg tcgtgtactg ctcgggcagc ggcgcctgga
    20341 gtcgcaacat ggtggtgaac ctccttgggg cctcgcgccg ggcgcgggcc gtgaggacct
    20401 ccgattgacg gccggtgggg gcagcctggt cgccacgcca cggggatgtc ggcctcagag
    20461 gttgatgtcg ccggaggcca ggccggcacc gagagcgtac ccctcacccc cgtcacccgc
    20521 cgacacatcg cccaaccccc gcgttctgac gacaagtcac gacagtggcg gtactcaagt
    20581 gtcatcagaa gatgaggatc caaaccgatc gctggttccg atgacagttg ggtaccgcag
    20641 cggtacgcgc gtgtcgtcag agcctttggt ggtcgggtgg cggcgcgggc gggagcgtcg
    20701 gacggcgcgg gtaggttcgg ccatgatgct cgagcgaccc ccggccggca ccatccccga
    20761 cacccccggc agctaccagt tcaaggacgc cgatggtcgg gtcatctacg tcggcaaggc
    20821 gaagtcgctg cgtcagcgcc tctcgaacta cttccagaac cccgccaacc tcccgccccg
    20881 cacgttccag atggtctcga cggccgagtc ggtcgagtgg atccaggtgc gcaacgaggt
    20941 cgaggcgctg atgctcgagt actcgctgat caagcagcac cggccccgct tcaacgtccg
    21001 tctggtcgac gacaagagct acccgttcct ggccgtcacc gtctccgacg agtggccccg
    21061 gcccatggtc atgaggggcc gccggcgcaa gggggtgcgc tacttcggac cgtacgccca
    21121 cgcctacgcc atccgcgaca ccctcgacga gctgttgcga acgttcccac tgcgcacctg
    21181 ctccgacgcc aagttcggcc gccacgagcg cctgggaaag ccgtgcctgc tgttccacat
    21241 cgagaagtgc gccggaccgt gcgtgggtga gatcgacgcc gagcactacc agcgccttgt
    21301 cgacgaactg ctcgagttcc tcgacggcga caccgaaccg gtgttgcaac ggctcgagac
    21361 ccagatggcc gaggcggccg aagcgctcga gttcgagcag gccgcccggc tgcgtgaccg
    21421 cctggccacg gtgcggcggg cgatcgagcg ccagcagatg gtcggcgacc gacgcgagga
    21481 cgtcgacgtg atcggcatcg ccgaagacga gctcgaggca tccgcgcagg tcttctacgt
    21541 ccgcaagggg agggtggtgg ggcgcttcgg cttccttctc gacaaggtga tggacctcac
    21601 cgccgagcag accgtcgccg cggtgctcga gcagctctac ggagacgaac cggctgtcgg
    21661 ctaccccaag tcggtgctgg tgcccgagct gcccgaagac ctcgacgtct accaggagtg
    21721 gttgaccctc cagcggggca gccgggtcga catccgggtc cctcagcgag gtcccaagcg
    21781 ggagctggct gagaccgtga cccgtaacgc caccgaggcg ttcacccgcc atcgactcaa
    21841 gcgcgcctcg gaccacaaca gccgggccaa ggcgctcaac gagctccagg agaccctcgg
    21901 cctgcccacc gccccgctgc gcatcgagtg ctacgacatg agccatctcc agggcaccga
    21961 ctacgtcggg tcgatggtgg tgctcgagga cggcttgccc cgcaagcagg agtaccgacg
    22021 cttcaagatc cgtggcgtcg acggcaacga cgacttcgcc gccatggaag aggtgttgac
    22081 ccgccgactg tcgaactacc tggcggaccg cgacaagccg tcgtccgaga aggggcgctt
    22141 cgcgtatccg ccgcagctgc tgctcgtcga cggcggcaag ggacagctcg gcgtcgcggt
    22201 caaggtcctc gaggacctgg gcctgagcga tcagatcccg gtggcctcgc tggccaagcg
    22261 cttcgaggag gtgtacctgc cgggtcggtc cgatcccgtc gaggtgcgac ggggttccga
    22321 ggccctcttc ctgttgcagc gcatccgcga cgagtcgcac cgcttcgcca tcacctacca
    22381 ccgggagctg cgcggcaagc gcatgacccg ctcggtcctc gacggcatcc cgggtctggg
    22441 cgagaagcgc aagcagcgcc tgatcaagga gctgggcggc gtccgggcgg tccagaccgc
    22501 cgagctgtcc acgctcgagg cgctgccgtg gcttcccgac gcggtcgccc gtgcgctcca
    22561 cgacacgctg cactcgccgg tgccgccgtc ggcgccgtag cgtcgggcgc cgtgcctgac
    22621 ccgacccaga accccgactc cggcgagcat cccgacccgt gggagacaca tgccgcctgg
    22681 tggcaggagc agttcaccga cggggtcgac cccgagtaca ccgaacagat cctgccgctg
    22741 atcgccgccc atctccccga gggggccgac cgcgtgaccg ggctggcgat cgacgtcggc
    22801 tgcggcgagg gccaggtcgc ccgggtcgcc gccgcggtcg gcctggacgt cctgggggtg
    22861 gacccggcgc agtcccagat cgacgtggcg gtgcgacggg gaggcggccc gcgctaccgg
    22921 aagggatccg cggtcgacct tccggcgggg gacggagccg ccgacgtggc gatcgcctgc
    22981 ctggtgttcg agcacatcgc ggacctcgac gcagcgctgg ccgaggtcgc ccgggtgctg
    23041 cgtcccggag gtcgcttcct gttcctgctc aaccatccgc tcctccagac accgggaagc
    23101 gggtggatcg acgaccaggt cctggacccg cccgagcagt actggcgcat cgggccctac
    23161 ctccgtgagg ccgagacgat cgaggaggtg gagaaggacg tgttcatccg cttcttccac
    23221 cgaccgctgt cgcgctacct caacgccgcc cacgacgcgg ggctggtgct cgagcgcatg
    23281 gaggagcctg ctccgccgcc agggttcctg gcccgcgccg aggagtacgc cgacgcggcg
    23341 tcggtgccgc ggctgctgct cctgcggttc acccggacct gagcccgggt cccggcacca
    23401 cgccggagca tggcgccgaa gtgcgggtgc ggccgacggt ccgaggatcg tcggcaggcg
    23461 gtgggtctca gcagtcggtg ggcctcagca gtctgtggcc ggagcggtgg tcacgccgtc
    23521 caccttgagg atcgtgccgt tgacggcctt gacgccgtcc tgggcgggca ccggggcccc
    23581 gccgaagtcg accgctgcgt catcgccctc gccctcgacg gtgaccttga cgttgcctcc
    23641 gagggtggcg acgcactgac cgttcttctc gctcagatcg tcccaggtga cgttgccgtt
    23701 gaggatgtgc agcttcagga cgttggcgag cgcacccctg gggtcctgct ccagtgcggt
    23761 catggtcgcg gccggcacgg tgtcgatcgc cgcgttggtg ggtgcgagca ccgtgaacgg
    23821 cccgccgtcg gtgagcatgg cgttgagacc cgcctcggtg gcgagacccg agaactccga
    23881 cagctgcggg tcggcgacca acaaggcggc gagcgtctcg gtcggtgccg cggtcgtggt
    23941 cgtcgtctcc ttctcggcct tgaccgtggt ggtcgcctcg gcgtccttgt tcttggagtc
    24001 ctcggtgctg ctgcaggcgc cggcgaacag ggcgaacgcg cagcagatgg cgacgacggg
    24061 caggaacagc ttgatgcgca tggacgatct cctcggatcc ccgggcgggc ccgggcggcg
    24121 atgggccctc aaacctagcg acggtcgggc cggtacccgc tgaacctagc gagggtggcg
    24181 gtgctcggtg tagatgggtc gatcggagcg gctcaccggt agcgtgagcc gggtgagcga
    24241 gttcgtggtg atcaccggcg tgtcgggggc gggtcgcacc cagttcggtg actccctcga
    24301 ggacctcggc tggttcgtca tcgacaacat cccggtcgag ctgatcccca aggtggccga
    24361 gttggcccgg ttcaaggagc ggggcacccc ggtggcgctg gtggtgggaa gcggggggga
    24421 cctggccgag atcggaccgg cgctcgacga gctgcgcggt tcgggagccg gggtccgcat
    24481 cgtgttcctc gacgcgtcga cccccaccct ggttcgtcgc tacggcgagt ccaagcgtcg
    24541 tcacccgttg gcagcgcagg ccggaaccgt ggccggggcg atcgacctcg agcgcgaggt
    24601 gctcgccgag gtgcgccagg ccgcggacat cgtgatcgac acatccgagc tcaacgtcca
    24661 tgacctgcgg tcccgggtca acgacctgtt cgccgagtcc gacggttcgc gcatgcaggt
    24721 caccctccag tcgttcggct tcaagcacgg cgtccccgcc gacgtcgaca ccgtgttcga
    24781 ctgccgtttc ctgcccaacc cgtactggat cgacgagctg cgcgacctga cgggactcga
    24841 cccggcggtg caggactacg tcaggtcgat gggtctcacc ggcgagttcc tgcgacgtat
    24901 cgaggacctg ttcgacctcc tcctgccggc gtacgtggcc gaggggaagt ccctgctgac
    24961 catcggcttc ggctgcaccg gcggccacca tcgttcggtg accatcgtcg agacggtcgc
    25021 ggaatggctc cggtccaagg ggatcgagcc gcgcgtccga catcgggaca tcagcaagtg
    25081 aacgccgacc acaacggttc agcacctgcc gtggtggcgg tgggcggagg ccacggcctc
    25141 gcgaccacgt tgcgcgcggc ccgtcgctac gccgggtcga tcaccgcagt ggtgaccgtc
    25201 gccgacgacg ggggctcgac gggtcgactc cgggccgcga ccccgcggcc cgcgcccgga
    25261 gatctgcgca agtgcctggt ggcactggcg gaccacgaca ccgcgatcgt caccgcgatg
    25321 gagcaccggt tcagcaccgg tgagctcgag ggccacgcgt tcgggaacct gttgctggtg
    25381 gcgctcgagg agtccgaagg tgatctggtg ggggcgctcg acgaggccgg acggatcctc
    25441 ggttgcgtcg gtcgggtgct gcccaacacc acgacggcgg tcgagctcaa cgcccgggtc
    25501 gactccggtg gagtggtgcg gggtcaggtc gccgtggggg agcgggccga ccttcgtacc
    25561 gtgtctctcg agccgccgga cgccccggcc tgcaccgagt ccatcgaggc gatcctctcg
    25621 gcggaccagg tcgtgctggg tcccggatcc ctctacacca gcgtcctggc ggccaccgcc
    25681 gtgcccggcg tcgccgaggc ggtccgccgc tcctcggccc gggtcgtgta cgtctgcaac
    25741 ctgcaccccc aggtgtcgga gactgcgggc tacggcgtcg cggaccacgt ggacgccctc
    25801 gcccgtcacg gcctgatccc cgaggtggtc ctctacgacc ccgacacgat ccgcggcgcc
    25861 gacggcgtcg tgggcgcccg gggcgtccgc ctggctgctg ccaacgggct ggcgcacgat
    25921 ccagtgctgc tgggcggcgc tctggccgca ctcgtcgcct gaagcacggt cgtgccgtcc
    25981 gcgacggctc gggattggca ctctcagcgt tcgccgtgct cgcatagggg tccgcgcccg
    26041 gtgtgatcgc ccggcgcgga ccagagtgcc atccgaccgt cagacacctg agacgaggac
    26101 gtgaaaatga ccgtgcgtgt ggggatcaac ggcttcggcc gcatcggccg caacttcttc
    26161 cgggcggcca aggcccgggg cgccgacatc gacttcgtcg ccgtcaacga cctgggttcg
    26221 ctcgaccaga tggccctgct gctcaagcgg gactcggtcg ccggcaccta cccgggcacc
    26281 atcaaggagg tcgacggcgg catcgacgtc gacggcgatg tcctcaaggt cgtccagcac
    26341 cgcaacccgg ccgagatccc ctggggtgac ctgggcgtcg acgtcgtcat cgagtcgacc
    26401 ggcatcttca ccgaccgcga caaggccgcc gcacacctcg acggcggcgc gccacgcgtg
    26461 atcatctcgg ccccggccgg cggcgtggac gccacgttcg tggtcggcgt caacgacgac
    26521 acgttcgatc ccgagaagca catcatcgtg tcgaacgcgt cctgcaccac caactgcttc
    26581 gttccgctca tcaaggtcct cgacgacgcc ttcggcgtgc agaagggcct gatgaacacg
    26641 atccacgcct acaccggcga ccagtccctg gtggacggtc cgcacaagga cctgcgccgg
    26701 gcccgggccg ccgcggtcaa catcgtgccc acctcgaccg gcgccgcccg cgccacgtcg
    26761 ctggtgctcg aggccatgaa gggccgcctg gacggcacgt cgctgcgggt gccgatcccc
    26821 accgggtcga tcaccgactt caccggtctg ctcgaccagg acgtcaccgt cgagcaggtc
    26881 aacgaggcgt tcaaggccgc cgcgtccgag ggcccgctca agggcatcct cgagtacacc
    26941 gacgagccga tcgtctcgtc cgacatcgtc ggttcgccct acagctcgat cttcgactcg
    27001 ggcctgacga tgaccatggg caacatggtc aaggtcctgt cctggtacga caacgagtcg
    27061 ggctactcca accgcctggt cgacctggcc ctggtcgtcg gcgccgccaa ccactgacgt
    27121 ccggacggac cggcgggtgg ctcgcacctc aggtgcaggc cacccgccgg aacgcgggac
    27181 ccatccgagg ggagaacgat gaagttcgga gtacccgagc tcgaggacct cggcgacgtg
    27241 gacggccgct ccgtcctggt gcgcgccgac ttcaacgtcc ccatccggga cggcgtcatc
    27301 accgacgacc tgcgcatccg cgctgcactg cccaccttgc agtggctccg ggagcgcggc
    27361 gcccgcgtga ccgcctgcac gcacctcggc cgacccaagg gtgcgccgga cccggcctac
    27421 tcggtcgagc cggtcaggca gcgcctggca gagctggcac ccggggtgga gctgctcgag
    27481 aacctgcgct tcgacccggg cgagaccggc aacgacgcgg ccttcgtcga ctcgctgtgc
    27541 gagggccacg acctgtacgt gaacgacgcg ttcggcgcgt cgcaccgggc ccacgcgtcg
    27601 atcgtcggtc cgcccacacg tctgccctcg gcggcgggtc ggctgctggc caaggaggtc
    27661 gaggtcctgc tcggactccg cgagcagccc cgccgtccgt tcactgcgat caccggcgga
    27721 tccaaggtgt ccgacaagct cggagtcctg accgccctgc tcgacatcgc ggatgacatc
    27781 gtcatcggtg gtggcatgtg cttcacgttc ttcggggcga tgggccatcc ggtggggagc
    27841 tcgctgtttg aggccgacca ggtcgacaac tgcgcccggc tcctcgacga gcacggtgat
    27901 cgcctccacc tgcccgagga cgtcgtcggc ctcgggcccg gcggcaagat cggggatccg
    27961 tccgccgggg gagaggtgcg caacttcggt cgctccctgc ccgacggctg gatgggcctc
    28021 gacatcggcc cgggtgccgc cgcggcgttc ggcgacgtca tcggcgagtc gcgcaccgtc
    28081 ttgtggaacg ggcccatggg cgtgttcgag gatccccgct tcgaggccgg gacgcgggcg
    28141 gtcgcccagg ccgtggccga gtgccgcggc ttcaccgtga tcggcggcgg cgactcggcc
    28201 gcggcgatcg cccagttcgg gctggccgac gaggtcgacc acatctccac cggtgggggg
    28261 gcgtcgctcg agctcatcga gcagggcgac ctgcccgggc tcgacgccct ccgccacgct
    28321 cccaacgtcg ccggctgaca ccggcacccg atccgcagct ccgatcccga gggggaacca
    28381 tggccagcac ccgcaagccg atcatcagcg gcaactggaa gatgcacaag aaccacttcg
    28441 aggcgatcca ggccgtgcag aagctgcact accgactcga tggcgacgac cacgacaacg
    28501 tggtggtcac ggtgcacccg ccgttcaccg acctgcggtc gatccagacg atcatcgaat
    28561 cggaccggat gaagatcgag ttgggtgccc agaactgcca ctgggaggac cagggcgcgt
    28621 tcaccggcga ggtggcgccg cccatgctgg ccaagctcga cgtcgcctac gtcatcgtgg
    28681 ggcactccga gcgtcgccag tacttcgccg agaccgacga gacggtcaac ctgaaggtca
    28741 aggccgtcca ggccgcgggg atgatcccga tcatgtgctg cggcgagacg ctcgagcagc
    28801 gcgaggccgg cgaggccgaa tcatgggtcg agggccagat cgttgccggc ctcgcgggac
    28861 tcggcggcga caaggtcgcc gcgctggtca tcgcctacga gccgatctgg gcgatcggaa
    28921 cgggccgaac cgccacctcc gaggatgccc aggccatgtg cgcccacatc cgctcggtgg
    28981 tgtcggccac cttcgggccc gacgccgccg cggcggtgcg catccagtac ggcgggtcgg
    29041 tcaagcccgg caacatcgcc gagctgatgt cgcagcccga catcgacgga gcgctcgtcg
    29101 gtggggccag cctggaaccc gacgagttcg cggccctcgt gcagtaccgg aaccactgag
    29161 caggtagcct gacccaccgt gtccatcatc gacatcgtcc tctgggtcct gcagttcgcc
    29221 ctcggcatcg gggtcatctt cctcgtcctg gtccattcgg gccggggtgg cggattgtcc
    29281 gacatgttcg gcggcggcct gggcgcctcc gcggccggat cgacggtgat ggagaagaac
    29341 ctggaccgga tcacggtcgt cgtcgggctc ctcttcgggt tcaacacgct gctgatggcc
    29401 ctggtcatcg acccgacctg acgactggtc cgacagccac ggccggccca gcggcgacgc
    29461 ccggtcaggg aacggtgacg gaaccgcgaa gctctcgttc ccggcgatgt cgtcggacgg
    29521 tgccgaccac atcgccgacc ccggccccca gcaacgcccc gaccatggcg tcggccggga
    29581 agtgcacccc ggtgtggacc cgggagtagg cgacggcccc ggccaggaaa tgcaccggga
    29641 aggccgccca cggcagctct gcgcccattg cgttggcgaa cgcgaacgcg gacgccgagt
    29701 gtccggacgg gaacgacggt gaggtgggca ggggcacctg gcgcccgacg ctgaccgcga
    29761 agtgctcccg atccgggcgc ttgcggggca tcaaacgctt gatcggttgg ttgaccacga
    29821 agctggtgat gccgatggcg atgacgccgt cgagcgcggc ccgacgtcca cgtcgcccgc
    29881 cgaccagggc ggttgcggcc gcgatgccga accagagctt ggagttgtcg gcgagcttgg
    29941 tgagacggcg gagagccgag tcgagggtcg gggtcgacgc ttcggcgacc gcgccgtaca
    30001 cggcgtggtc gagcgcctcc aggtcgtcca cgaccgtgcg ggcccactcg atgtcgtggg
    30061 cgtcgacgaa gtcgcggagc cgtgcggtca acccttgttc ggtatcgctg tcggtgtccg
    30121 gtccctggtc gctgaacgtg ctcacgggga cgagtgtcgc acgctgcggc ggctcggccg
    30181 gtgtttgcgt gtgggttcga ccttccacgc gtcgttctcg cacgcggagc ctggggtgcc
    30241 ggtgttcgcg t
//
LOCUS       DAOQOD010000014        28787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_516_length_28787_cov_12.900807, whole genome shotgun sequence.
ACCESSION   DAOQOD010000014 DAOQOD010000000
VERSION     DAOQOD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 28787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28787
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_28787_cov_12.900807"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..780)
                     /gene="sufC"
                     /locus_tag="PLZ72_02925"
     CDS             complement(34..780)
                     /gene="sufC"
                     /locus_tag="PLZ72_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNA35283.1"
                     /translation="MSELVISDLHAEVGDRPILHGVTVTVRSGEVHAVMGPNGSGKST
                     LSHVLMGRPGYRVTGGSVHLDGVDLLALPTWERARAGLFLALQYPTEVPGVRLADVLE
                     EAFVAAGRDPSTVPGRLRAEADRLGFDVGLLGRPLNVDLSGGEKKRNDTLQLSVLQPR
                     IAVLDEIDSGLDVDALRAVARRIEDASSRGIDADGDEPLGVLAITHYSRLLEELHPDV
                     VHIFVGGRIVETGGPDLVARLETDGYAAWT"
     gene            complement(777..1133)
                     /locus_tag="PLZ72_02930"
     CDS             complement(777..1133)
                     /locus_tag="PLZ72_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNA35284.1"
                     /translation="MTGSVDVAALDDLVDGEGTAFELDGRRVSVVRLGDHVYVVGDTC
                     SHEKVSLSGGIVDEDACTIECPKHGSEFDLRTGEALTLPAIRPIPTYAARVLDGRVLI
                     DVGAENDHTSAPEVSP"
     gene            complement(1130..>1588)
                     /locus_tag="PLZ72_02935"
     CDS             complement(1130..>1588)
                     /locus_tag="PLZ72_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNA35285.1"
                     /translation="DLRTFQTHAAPDTTSHLLFKGGVAGRSHSVYTGLIRIAAGARGA
                     HAEQTNRNLKLSEEAWAESVPNLEIENNDVRCSHASTVGPVDPDQRFYLESRGVPTDV
                     ADRLIVRGFFDEVLDDFATPALSAEIAGRLDATLVGSADGGVDRSSEGGS"
     assembly_gap    1589..1688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2092..2454)
                     /locus_tag="PLZ72_02940"
     CDS             complement(2092..2454)
                     /locus_tag="PLZ72_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35286.1"
                     /translation="MPMQESCKFFESRTYPNGETVRKCDLDLAPEAPWRCPDGCTAFT
                     PRLADVNWAHGTLVVPPTPPEPSSLGTDPSIAALLDEAEDIVNAAGPKMRAEVEADRT
                     ARPSGVERGWKRIFRRRR"
     gene            2565..>3534
                     /locus_tag="PLZ72_02945"
     CDS             2565..>3534
                     /locus_tag="PLZ72_02945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA35287.1"
                     /translation="MSDHAPDPVGFEPIADPRGGVVDVVGPMRRILDAASVPSATLDG
                     EGQIVGLNPEFARLCGRAPSEIVGLHLLALCPGRDQAEVLSRLVRIVGGLSETEREEL
                     RVTGADGRVRTLSVTLGGLAGPDSRVERVVAIASDLTEERRAARRRRRQVVARATDAV
                     ADQQTGLPNQRALPVLVGSALRRSATHGAPFALLRCHVTNLAEIERTHGPVVTRNAMS
                     ALSERLTQRLRSADTVTRSHPDAFTVIAEDLGDAQDAAGVAYRLLASVVEDVVVDGRA
                     VGFDMVIGVSVGDGDGDPDRLTDDATRAVEDARADGIGGFRMIDTRT"
     assembly_gap    3535..3634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3685..5079)
                     /gene="sufB"
                     /locus_tag="PLZ72_02950"
     CDS             complement(3685..5079)
                     /gene="sufB"
                     /locus_tag="PLZ72_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNA35288.1"
                     /translation="MSVDLDLSKYSLGWSDEEDYVFKPKRGLDEAIVREMSWMKGEPQ
                     WMLDFRLKSLRAFERRPMPNWGGDMSEIYFDDIYYYIKPTDSQVDEWDDLPEAIKSTY
                     EKLGIPEAERKYLAGVTAQYESEVVFHRNREDLERQGVIFCDMDTALREHPEIVKQYF
                     GTVIPRNDNKFSALNSSVWSGGSFIYVPPGVEVDMPLQAYFRINAENMGQFERTLIIA
                     DKGSKVHYIEGCSAPVYSSDSLHSAVVEIVVKEAARVTYTTIQNWSSNVFNLVTKRAR
                     VEAEGHMEWIDGNIGSRLTMKYPSVVMVGPKASGEVLSVAYAGAGQHQDTGSKMIHAA
                     PETTSKIVSKSISKDGGKTSYRGLVKVDDGLTGCRSHVQCDALILDDHSISDTYPYME
                     VGSRDAVIGHEATVSRVADEQLFYLMSRGLTEEQAMSMVVNGFIEPVTRTLPMEYAIE
                     WSRLIELQMEGSVG"
     gene            5217..5720
                     /locus_tag="PLZ72_02955"
     CDS             5217..5720
                     /locus_tag="PLZ72_02955"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNA35289.1"
                     /translation="MTVTEGEPTAPTAPEPPPLGWEPPAPLPRTTRPRWVAPAATGAA
                     LVAGCAAIALWDPGDGGVAVCWSKALFGIDCPLCGGLRATNAMMRGDWLAAADHNVLV
                     AVGLPLLAVLWAVWLIRSIQDRPLDLPRFPRAAVIGFGVFLLAFAVVRNLDVDVAWIH
                     WLASDTA"
     gene            complement(5814..6518)
                     /locus_tag="PLZ72_02960"
     CDS             complement(5814..6518)
                     /locus_tag="PLZ72_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35290.1"
                     /translation="MRRHLSTLAIALTAVTVAVGVLPATSAGAQGCAGDRVTASMSSS
                     SVSGPAWSVTGACVDARDYFVGEAQGHAFAIGISTVTYDDGHVGRLAFLSVRVDAAQA
                     VTVLAMEDPRTGQLHFAGTVRQAAIGVTGATVSGAGIGFPPLESATVSLDFADAAAPW
                     PDWATGSTTVPAESPTSLPGTETTTSLPAEAPLGATTVAPGVSTPTTTVPSVTSTLPS
                     VTSVPTIPGVDPQPVP"
     gene            complement(6727..7245)
                     /locus_tag="PLZ72_02965"
     CDS             complement(6727..7245)
                     /locus_tag="PLZ72_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNA35291.1"
                     /translation="MPIYALGDQEPEIHPSAYIHPDAVVIGSVVIGAESSVWPCAVLR
                     GDDGFIFIGERTSVQDGAVLHTTPFWPTTVGNEVTIGHLAHLEGCTVEDKALIGIGSK
                     VLHNATVGREAIVGGGAFVGNNKVVPPLAMALGIPAVIREDAVTPGHFDLGVQSYVDR
                     RERFRTHLRRLD"
     gene            complement(7275..7802)
                     /locus_tag="PLZ72_02970"
     CDS             complement(7275..7802)
                     /locus_tag="PLZ72_02970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNA35292.1"
                     /translation="MTSDSAVTEPAESPIEIDPMLYRQVLGHFPTGVTVISSQVEGRP
                     VGLAIGSFFSVSLDPPLVGFCVSRTSSTWPLIRSTGVFGVSVLAEDQTETSGRFASKA
                     DDKFQGISWDPSPITRSPLIHGAVAHIDCEIHEIHPGGDHVIVEGLVRSLDVHRPDVG
                     PLLFFKGGYGRHAKI"
     gene            complement(7812..8711)
                     /locus_tag="PLZ72_02975"
     CDS             complement(7812..8711)
                     /locus_tag="PLZ72_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA35293.1"
                     /translation="MLDGPGRSPIILDLGTGLRVYGAELTRRDMDGAVGEPFRGTALL
                     THLHWDHVQGIPFFAPLLSAGAELDLHAPVQAGEPLADVVRGFLSPPYFPVELEALPG
                     QIRFHDTEPGRFRVDDATVTAAWVPHVGPTFGYRVERDGRSVAYVSDHQQPTAGPTAV
                     DPAVLELCRDVDLLIHDAQYDDAEFAQRPDWGHCTVDYAVEVAAASGAHTLALFHHDP
                     GHDDRRVDELLVSARELADLRGVPEVIAAHEGLTVTLTPRVELARSVPDGRSGAREPS
                     GATGQESVGVGADAAFPQVAVGR"
     gene            complement(8869..9816)
                     /locus_tag="PLZ72_02980"
     CDS             complement(8869..9816)
                     /locus_tag="PLZ72_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNA35294.1"
                     /translation="MRGPCGSDVALTRDLTAEQEAVLDGGMPRLRDLPWRRTRDPWAV
                     LVSEVMLQQTPVARVAPRWERFLDRWPTIASAAEAPLRDLLVDWKGLGYPRRARYLWL
                     SARACVDRHGGSVPSTLPDLLALPGVGPYTARAVMAFAGGSAVGVVDTNIARVLARRA
                     GRGLTAGAAQELADAWVPRERAWEWNQVLMDLGATRCRPIPDCDGCPVDCAWRDAGFP
                     DPDPAQGSAGVSRRQAPFAGSDRELRGRVLDHLSDGPRSRASLERLLVGNTVDPQRLV
                     AALDGLVRDGLVVANRDDTFDLPRGETGPSEISVTAHGT"
     gene            complement(9783..>10560)
                     /locus_tag="PLZ72_02985"
     CDS             complement(9783..>10560)
                     /locus_tag="PLZ72_02985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35295.1"
                     /translation="VPVNPVAAGGFSAAASTYARIRPTYARPAIGAIKDLAPRGGRVL
                     DVAAGTGILTGQLLRAGLDLWAVEPLPAMAAQLRLSLPAVPVALGLAEALPVVDASVD
                     VITVGQGFHWFDAGAALAEAARVLRPDGVLALVWNARDESTEWVDQLTRLVEDRTGGR
                     PYVDHRERPWAEVVESSGRFEPLVERRYPNPVPTTVEGVMDRLRSTSFVAALDEGPRE
                     DLLEEAADLLDSHPELTGTFAYPHETVLHTCGVRAARTWR"
     assembly_gap    10561..10660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10661..13132
                     /locus_tag="PLZ72_02990"
     CDS             <10661..13132
                     /locus_tag="PLZ72_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA35296.1"
                     /translation="LVIGAAIMVLPMFGAHRLQIGAVILSAATVSNMALFRRARRGGS
                     VPSAVAIADLALACVVATIVPASYGVVVVILVSSAGLYVFWFGPRTVWRLMPFTAAAL
                     AAIGIRSGSNYWVPFFVAWCVTTTFSTIIFGHLARTLAAQRDRFDELVNGIDAAVWEA
                     PGPAGPADFLSKHVVDLLGFERDRVLDPEFFLSRVHPEDRSVFVEGQQLVAGGQDVEV
                     HFRVTDAWGRVRRLQNRVRVTLDGTGRVVRRRGIVVDETARWEAEATVRQYSDFVEGI
                     PIALVVIQMTDPTDRSSFVLRAANPAANALADLDDRAIGHRIADLISVTDAFLGDMSD
                     VVASGEPHDEPFVQLAGVDEIFSMRAVPLPDRCVGISLEDITKRARTAEAFRHQATHD
                     ALTGLPNRALLHERLTRALIEADRTGAPVGLLMVDLDQFKDVNDALGHEYGDALLREI
                     ARRMSDRLRNCDTIARLGGDEFAVLLSTDASMSGAEEVARRLNELATEPVQVGQYRLQ
                     VSASVGIALAPEHGNDAETLMRRADGAMYDAKQAGGGHVVYCAAQELAAVKRLELLAD
                     LRGDGFGEQMEVHFQPRVQLSSGATVGIEALVRWRHPRHGLLAPAEFIELAEVSGAIR
                     GITQAVTSRTWDALTELERHGIRSASVNLSTRNLFDPAWVEWMCEMAETRGIGEGRLW
                     MELTEGQLMDDPRQSTEVIERLHRIGIRFSIDDFGSGASSLAMIRDLPVDEVKIDRRF
                     VDELLDGDDRITRSIVDLAHHLGLSVTAEGVADPRLIETLRSIGCDTVQGFALARPMP
                     LDELRTHLVREQAEGLEDARYRPVT"
     gene            13138..14745
                     /locus_tag="PLZ72_02995"
     CDS             13138..14745
                     /locus_tag="PLZ72_02995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35297.1"
                     /translation="MTSADLPSPDLDAVAAALDVAQAVIDGGIARLVDGGIDQNQVLA
                     YDVAHAAAGIGASRSLLAYGAQGDVQARIACVFAAEALADLAGKVFGREADWGAAPGA
                     LDGARQATAAYRSPAFQASLAGTDGPRGLADDFEMVQDTFRRFAEDKIAPIAEHIHRH
                     NDDIPEEIITGLAEMGAFGLSISEEYGGFATGTESDYIAMIVATEELSRGSLGAGGSL
                     ITRPEILARALETGGTEEQKQHWLPQLASAEVMNAVAVTEPDYGSDVAGVKVTAASVE
                     GGWLINGVKTWCTFGARADVLMLLARTDPDRSRTHRGLSMFIVPKERGDGHGFTQTQD
                     NAHGGGKMEGRPIDTIGYRGMHSYEIALENWFVPSENLIGGEAGLGRGFYYQMAGFEN
                     GRLQTAARAVGVMQAALEEARQYAEDRKVFGQSVGDYQLNAAKLGRMAATVQGARQFS
                     YDIARQVAGGEGRLEAALLKAYVCKAAEWVTREAMQIHGGMGYAEEYTVSRLWVDARV
                     LSIFEGADEVLCLRVIAKQLVERAAAD"
     gene            complement(14785..15282)
                     /locus_tag="PLZ72_03000"
     CDS             complement(14785..15282)
                     /locus_tag="PLZ72_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35298.1"
                     /translation="MALTEKSRSALYLGLSHVIDEEAVEEMLSYFPARDVEESVTKEF
                     LRAELAELEMRLSESLRSELQSEVGALRGEVQELRGEFRSELQSEVGSLRSELQSEVG
                     SLRSELQSEVGSLRSELQSEVGSLRSEMRAMRSELRDEMRRTVLINIGTLVAFAAVII
                     TAVRI"
     gene            complement(15424..16845)
                     /locus_tag="PLZ72_03005"
     CDS             complement(15424..16845)
                     /locus_tag="PLZ72_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HNA35299.1"
                     /translation="MQTGNFINGEFRAALSGATDDVVAPATGEVIATVASSGAADVHA
                     AVAGARAAFETWSETTPRARFEMITALADAIEGDLENLKALESSNVGKPVSIIDFEFD
                     LTVDNFRFFAAAARFLEGRAAGEYLEDHTSFVRRDPLGVVASIAPWNYPLNMATWKIG
                     PALATGNTVVLKPSELTPLTAIRLAELAADIFPPGVFNVVLGQGESAGAALVAHPDVA
                     MVSITGDVSTGKLIARNAAETLKRVHLELGGKAPVIVFDDADPAAVVECLTDMSYYNS
                     GQDCTAPCRVIAGPRVFDDVVSGLTDSVAALRWGDPSDPDVAVGPVISAEQQAKVAGM
                     VERAAAAGAEVTVGGAVGDREGFFYQPSVVVGPEQSSEIVQREVFGPVVTVQRFDDEA
                     QALQWANGVDYGLASSVWTNDVARALRMAKRLQFGTVWVNDHIPIVSEMPHGGYKQSG
                     YGKDMSIYALEHYTEIKHVMVRI"
     gene            complement(16958..17650)
                     /locus_tag="PLZ72_03010"
     CDS             complement(16958..17650)
                     /locus_tag="PLZ72_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNA35300.1"
                     /translation="MDALRHPDARIAAVAALEQPLRRRLYDLLVERAGWVGRDDAAAA
                     LGVPRSVAAFHLDKLAALGVVEVDYERAAGRTGPGAGRPAKRYRLTVDEVGASVPDRR
                     YDLAGRLLADAVAAATEHGEPVGEALTRGARALGLEIGRSVADADDLTGWHAVIEALI
                     EHGYQPCVGDDDVVLDNCPFHRLAEEHRDLICGMNLDVLRGVIDGIGASPTYRATLAP
                     EPGRCCVRLDRV"
     gene            17765..18334
                     /locus_tag="PLZ72_03015"
     CDS             17765..18334
                     /locus_tag="PLZ72_03015"
                     /inference="COORDINATES: protein motif:HMM:NF021505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HNA35301.1"
                     /translation="MRKFSARPTLTLKGRTFKGPRGWSGKPSHPPLTDIPIGAYLLVA
                     GFDVVSAIAGDSHGWAGDLWRTGTWILAAGLAVSVLAALTGLADARSSSEAGTQARRT
                     INTHATFMVTATLVALADLVWRLAVHDTALVTPVGIVVLSVIVAGLVTVGATFGGSLV
                     FEYGFNVETAGDHPVWHRSETDVLPGQDH"
     gene            complement(18356..19234)
                     /locus_tag="PLZ72_03020"
     CDS             complement(18356..19234)
                     /locus_tag="PLZ72_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNA35302.1"
                     /translation="MTDDLRPRRSALYMPGANDKALEKAKSLPTDAIIFDTEDSVAPD
                     MKAVAREKVAAAAQSGEYGSRELTIRVNSLDTEWAVDDLRSAAQAGPDGIVVPKVNSA
                     ADVAEVERIIEAAGVPDHTRIWAMLETPAAVEAAVEIATSSERLAVLVMGTNDLAKEL
                     RAGLVPGRAPLLWGLARCLNAARFADKVILDGVYNDVRDPDGFAVEAAQGAEMGFDGK
                     TLVHPTQVEPANAAFSPSADEVEYSRRVIEAFEQGLAEGKGVITVDGRMIENLHVDNA
                     RRAIAIADAIAALSDS"
     gene            complement(19255..20316)
                     /locus_tag="PLZ72_03025"
     CDS             complement(19255..20316)
                     /locus_tag="PLZ72_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNA35303.1"
                     /translation="MRSPKDFFAPLAVGAPEPRREIPFPPSRMIHFFPPHLEKLTAKL
                     PEIAPTTDILLGNLEDAIPMDAKEAARAGLVKVAKEMDFGSTQLWTRVNSLDSPWVLD
                     DLVTLVGEVGDKLDVIMIPKVEGPEDIHYVDRLLAQLEAKAGLTEPLMVHAILETARG
                     MNNIEEICNASPRMQGLSLGPADLAASRRMKTTRVGGGHPGYLVRQDPPQGDDGPAYG
                     AERTSYQQDLWHYTIARMVDACAAAGILPFYGPFGDIKDTVACEDQFRNAYLLGCVGA
                     WSLHPVQIDIAKKVFSPDPADVAHAQRVIEAMGDGTGAIMLDGKMEDDASVKQCHVVV
                     NLARQLAERDPSLAERYGF"
     gene            complement(20396..21847)
                     /locus_tag="PLZ72_03030"
     CDS             complement(20396..21847)
                     /locus_tag="PLZ72_03030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA35304.1"
                     /translation="MSPVRPLRSTTATAAVIDASLVDAALADSQLSVLWTDTDDRPAV
                     GAPLDGDERADLVVVGAGYTGLWAALHSLESEPSRSVLVIDSGPVAARASGRNGGFVS
                     ASLTHGLANGLDRFGSDIDRILAAGRTNFDGLVADVRRHEIDARLELVGALAVATEPW
                     CVPVVRRDADQAVMHGEDVELFDADGIRALVDSPTYLSGSWTRSGEALVDPARLGWGL
                     ADAVRRAGGRIAEGTTMTGLVRHGAGVEVRTDRGRIRAQAVVLGTNAERSPVRAINRR
                     IVPVYDHVLATEPLTEQQRASIGWRGRQGISDTTNQFHYYRLTDDGRILWGGYDAVFH
                     NWGRTDERFEDRPETFRTLAGNFLRTFPQLEGIRFSHRWAGVIDTSTRFSVGFGTALD
                     GRVAYAVGYTGLGVAASRFGGRVCVEMLFAPEAPDLDLDLVRRAPIPFPPEPIRTAGI
                     QLTRRAIARADADGGRRGPWLRLLDRLGLGFDS"
     gene            21902..22897
                     /locus_tag="PLZ72_03035"
     CDS             21902..22897
                     /locus_tag="PLZ72_03035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNA35305.1"
                     /translation="MRAVMCRELVDPEELRVEELDNVPCGPDRVRVRIWASGVNYVDA
                     LFVQGQYQIKPPLPFIPGSEIAGELTEVGPEVERWEVGDRVMASVGLGGFADEIVLRA
                     DQLTRIPDRLSFGQAATMGQSYCTAWFTLTRRTVLRPEDWVVIFGAAGGVGLAMLDVA
                     RSFGAHTVAVASSPEKLQLAIERNANAVIDSSDAGSDGATMRARIREVTDGGADIVID
                     PVGGTLTEEGLRGLREGGRLMVIGFASGSIPTLPANQVLLRNRSVLGVDWGAWALGHP
                     AENAALLGEVLAQVDAGALEPVEPTMVPLEDAGTALRDLIERRVVGKVALESRAR"
     gene            complement(23030..23809)
                     /locus_tag="PLZ72_03040"
     CDS             complement(23030..23809)
                     /locus_tag="PLZ72_03040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA35306.1"
                     /translation="MTGSSHKRDPHGGADPYSKLSHAQDRRRRTIPMVIGGTVVVVAI
                     CALVAILATRSDDTDSGAAAGTGAAAAANAEQETADITVTGEPLPSMPEMAPGSYFTD
                     VSTDKAIGMPAPSIDGSSFDGTPVKVDTGDGTPRVIVFVAHWCPHCQKEVPAIQQWID
                     DGNLPKGVEIVYVSTGVAPERGNYPVSDWIEKEGVTPKVVLDDDQSAAASDYGLSGFP
                     YFVMIDGQGKVVARGSGEIPMDVFGPAVDALAAGQDPTAAS"
     gene            complement(23806..24384)
                     /locus_tag="PLZ72_03045"
     CDS             complement(23806..24384)
                     /locus_tag="PLZ72_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNA35307.1"
                     /translation="MSNDTVSLFLAMLAVLALAFVVASAVVALLARTRTLGPGLAAWR
                     DAASDASLLLALGVAATCTLGSLYMSEVRHFVPCDLCWYQRIAMYPLVVVLAVAVVAR
                     DKAVWRYVLPIALIGAAVSTYHYLHERFPESVSTACSLEASCSTLWIWEFHFLSLPGM
                     AWVGFITICTLMLIARSALRREAGPDPQEVPA"
     gene            24547..25032
                     /locus_tag="PLZ72_03050"
     CDS             24547..25032
                     /locus_tag="PLZ72_03050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNA35308.1"
                     /translation="MSVYDTELTALDGTPGVLNDQDGKVTLLVNVASKCGLTPQYTQL
                     EELHERYSDRGFTVVGVPCNQFMEQEPGSPEEIATFCSATYGVTFPLTEKVEVNGDGR
                     HPLYVELTAVADEDDGSVGDIRWNFEKFLIGRDGKVLARFNPTVVPDDPKVTGAIEAA
                     L"
     gene            complement(25051..26169)
                     /locus_tag="PLZ72_03055"
     CDS             complement(25051..26169)
                     /locus_tag="PLZ72_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HNA35309.1"
                     /translation="MRAALFTEVGRPLDVVDDIDIQGPGVGQVRVRIAACGVCHSDLS
                     LVQGHIPLPGPTIPGHEAAGEVVELGPGVDSLAVGDHVVLSPNPACGRCEYCRRGEPS
                     VCTGSISIAMPMFPDGTTHLSRPVGSGAGGRSDVVYRGLGLGAWAEEVIVAASGAVAI
                     PVDVPLDVACVIGCAVQTGVGAAINTAAVQPGDHVLVMGAGGIGISIVQGAAIAGAAR
                     IVVSDPSESRRELAMRFGATDVIDPDETDVVGRTMEITGIGADVAFDAVGSATLVEAG
                     LQAVRSRGTVVMVGAPPPEQDASVNVALTMFTEKRLVGSLLGSCHAPRDIPRLVDLWQ
                     SGRLDLDAMITARRPLTEVNEALDDLAAGRGLRTVLVP"
     gene            complement(26229..>28787)
                     /locus_tag="PLZ72_03060"
     CDS             complement(26229..>28787)
                     /locus_tag="PLZ72_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA35310.1"
                     /translation="KDPIVIGIVLLINAVLGYVQESKADDALAALQQMLEVTVRVRRD
                     GGMLELPASELVPGDVVVMEAGDRVPADGRFLVARSLSVDESTLTGESVPVDKTSDVI
                     EVDTDSGGDVPLAERANCGYMNTTLTRGRAEMVVTATGMSTEVGRLAGLLNTAEDSQT
                     PLQEQLDRLGKRLAMIAGVAVLAVFLVGLSQGDEIGDAVLGAVALAVAAIPEGLPAVV
                     TVTLAIGVSRMAAERAIVKRLASVETLGSTTAICSDKTGTLTLNQMTATRVVAAGRTF
                     GFSGLGYGDGGDVSVIAGSPEPDPTTDDALRWALTTAVLCNDAHVRTGDYGEAQLVGD
                     PTEGALVVAARKIGLDPDAVRSEVPRRAEVPFDSAVKFMATLHPMDDQGSLLVVKGAP
                     DVVLSRCTARRLADGVVPLDGDGRQRAMDDNDALGVEGLRVLAVATKVLGDRVPTVAG
                     TDPTGSDAEELDLSAEIDGLTLETLVGILDPARPEAVAAVAECRDAGIGVRMITGDHA
                     TTAGAIAAELGIPGRVITGAELNDMSDDDLAAQIEEIGVCARVSPEHKVRVVKALQSN
                     GEVVAMTGDGVNDAPSLKQAEIGVAMGITGTEVTKEAGDMILTDDNFATIVKAVERGR
                     AIYENIVTFVRFQLTTNIAAIATILTARLAGYPAPFNPIQILFVNILADGPPAMSLGM
                     DPPRPGVMDRPPRDREERILSGARLVPILLIAAVMTVTIMAILIGWSDSAPGTAPEDD
                     PAFTMAFTSFVFLQMVNALVVRSGDRSVFNRFSVTNRTFWYAVGGVVAAQVLIVVIPF
                     LQNVFGTTSLTGEQWAVVVVSTLPLLVLAELATLVGRFRRHRRPRELAPAAVSS"
BASE COUNT         4317 a        10030 c         9975 g         4165 t
ORIGIN      
        1 gcggattgcg acaaggtgtg tcgctgagca cccctatgtc caggcggcgt agccgtcggt
       61 ctcgaggcgg gcgaccaggt ccggaccacc ggtctcgacg atccgacccc cgacgaagat
      121 gtgcaccacg tcggggtgca gctcctccag cagccgcgag tagtgcgtga tcgccagcac
      181 gccgagcggc tcgtcaccgt cggcgtcgat gccgcgtgag ctcgcgtcct cgatccgacg
      241 ggccaccgct ctcagcgcgt cgacgtcgag acccgaatcg atctcgtcga ggacggcgat
      301 gcggggctgc agcacgctga gctgcaaggt gtcgttgcgc ttcttctcgc cgccggacag
      361 gtcgacgttc agcggacgcc cgagcagacc gacgtcgaac cccaaccggt ccgcttcggc
      421 ccgcagtcgt ccgggcaccg tcgagggatc gcggcctgcg gccacgaacg cctcctcgag
      481 cacgtcggcc agccgcacgc ccggcacctc ggtcggatac tgcagcgcca ggaacagtcc
      541 ggcccgggcg cgctcccagg tcggcaacgc cagcaggtcg acgccgtcca ggtgcaccga
      601 tccgccggtc acgcggtagc cgggtcggcc catgaggacg tgactgaggg tggacttgcc
      661 cgacccgttc ggacccatga cggcgtgcac ctcgcccgag cgcacggtga ccgtcacccc
      721 gtggaggatc ggccggtcgc ccacctcggc gtggaggtcg gagatcacca gctcgctcat
      781 ggggacacct ccggtgccga cgtgtggtcg ttctcggcac cgacgtcgat gaggacccga
      841 ccgtcgagca cccgggccgc gtaggtcggg atcggccgga tggccggaag cgtcagggcc
      901 tcgccggtgc gcaggtcgaa ctcgctgccg tgcttggggc actcgatggt gcaggcgtcc
      961 tcgtcgacga tgccgcccga caacgagacc ttctcgtggc tgcaggtgtc gccgacgacg
     1021 tacacgtggt caccgagccg cacgaccgac acccgacgtc cgtcgagctc gaacgccgtc
     1081 ccctcgccgt cgacgagatc gtcgagggcg gccacatcga cgctgccggt cacgacccgc
     1141 cctccgaact ccggtcgacg ccaccgtcgg ccgatcccac gagggtggcg tcgagcctgc
     1201 ccgcgatctc cgcggacagc gccggggtgg cgaagtcgtc gagcacctcg tcgaagaagc
     1261 ccctcacgat caggcggtcc gccacgtcgg tcggcactcc ccgggactcc aggtagaagc
     1321 gctgatccgg gtcgaccgga ccgacggtgg atgcgtggga gcagcgcacg tcgttgttct
     1381 cgatctcgag gttgggcacc gattccgccc acgcctcctc ggagagcttg aggttccggt
     1441 tggtctgctc ggcgtgggcg ccccgggctc ccgcagcgat acggatcagg cccgtgtaga
     1501 ccgagtggga gcgaccggcc acgccgccct tgaacaggag gtgcgaggtg gtgtcgggcg
     1561 cggcgtgggt ctggaaggtg cgcaggtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnncc gctccgtcga cgaggaccac gacgcccgcc aggtccccca gcgccgccac
     1741 catcgacgac gcagcggcag gaacctcgcc ggcggcagcc tccgacgacg cggcgccgta
     1801 tcgggccaca tcgagctcgg cgatgcggct gtagcgccac tcctcggcct cgacggtggg
     1861 caacgggaga tgagcggccc gggtccgcgc ctgggaccga cgtcggacca accaggcggg
     1921 acccgatgga tcgtcgacca cgaacggcgg tggaacaggt cccgggccgg tcacgagttc
     1981 ggcggaacca acgggaggca cggcgtcagg tcgaggcggc accgggcgaa tttacactac
     2041 gccaataggg gaatctccag ccgacgtatc cagtcgtgcc ggtccaggtg ctcagcgacg
     2101 acgacggaag atccgtttcc agcctcgttc cacgcccgac ggtcgagcgg tgcggtccgc
     2161 ctccacctcg gcacgcatct tcggcccggc cgcgttgacg atgtcctccg cctcgtcgag
     2221 gagtgcggcg atcgaggggt ccgtccccag gctgctcggc tcgggcggag tgggaggcac
     2281 caccaaggtc ccgtgggccc agttgacgtc cgcgagcctc ggggtgaagg ccgtgcaacc
     2341 gtccggacaa cgccacggcg cctccggtgc caggtccagg tcgcacttcc gcaccgtctc
     2401 cccgttcgga taggtgcggc tctcgaagaa cttgcagctc tcctgcatcg gcatggcgcc
     2461 cagtgtgtca gcgtcggagt cggatgcggc accgacgggt cgcgacgctg ggtagaacct
     2521 cccgaatcct cagggctgcc gcgaccgggg ccgacaactg gaccatgagc gatcacgccc
     2581 cggatcccgt cggcttcgag ccgatcgccg accctcgcgg tggtgtcgtc gacgtcgtcg
     2641 gaccgatgcg acgaatcctc gacgcggcgt cggtcccgtc ggccaccctc gacggcgagg
     2701 gtcagatcgt cgggctcaac cccgagttcg cccggttgtg cggccgggca ccgagcgaga
     2761 tcgtcgggct gcacctgctc gccctctgcc ccggacgcga ccaggccgag gtgctctccc
     2821 ggctcgtgcg gatcgtcggt gggttgtccg agaccgagcg ggaggagctg cgggtcacgg
     2881 gagccgacgg aagggtccgc acgctgtcgg tcaccctcgg tgggctggcc gggcccgaca
     2941 gcagggtcga gcgggtcgtc gccatcgcgt cggacctcac cgaggagagg cgggcagccc
     3001 ggcgccggcg acgccaggtc gtcgcccggg ccaccgacgc ggttgccgac cagcagaccg
     3061 gacttcccaa ccagcgtgcg ctgcccgtcc tggtcggctc tgcactgcgc cgctcggcga
     3121 cccacggcgc accgttcgcc ctgttgcgct gccacgtcac caacctggcc gagatcgaac
     3181 ggacccacgg tccggtcgtc acccgcaacg cgatgtccgc cctgtcggaa cgactcaccc
     3241 agcggctccg cagcgccgac accgtcaccc gctcccaccc cgatgcgttc accgtgatcg
     3301 ccgaggacct cggtgacgca caggatgccg caggcgtcgc ctaccgactc ctggcctcgg
     3361 tggtggagga cgtggtcgtc gacggccgtg ccgtcgggtt cgacatggtc atcggcgtct
     3421 cggtcggcga cggcgacggc gatcccgatc gactcaccga cgacgcgacc cgagccgtcg
     3481 aggatgcccg cgccgacggc atcggaggct tccgcatgat cgacacccgg accgnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccctcc tctcccccga ccagcccacc
     3661 cctcacggtg tgggcttcgc gggctcagcc cacgctgcct tccatctgta gctcgatgag
     3721 gcgggaccac tcgatggcgt actccatggg cagggtgcgg gtgaccggct cgatgaagcc
     3781 gttgaccacc atcgacatgg cctgctcctc ggtcagcccc cggctcatga ggtagaagag
     3841 ctgctcgtcg gccacccgcg acaccgtcgc ctcgtgtccg atcaccgcgt cgcgggaccc
     3901 gacctccatg tagggatacg tgtcgctgat cgagtggtcg tcgaggatca gggcgtcgca
     3961 ctgcacgtgg gaccgacaac cggtcaggcc gtcgtcgacc ttgaccaggc cgcggtacga
     4021 ggtcttgccg ccgtccttgg agatcgactt ggagacgatc ttggacgtgg tctcgggcgc
     4081 ggcgtggatc atcttcgacc cggtgtcctg gtgctgaccg gcacccgcgt acgcgaccga
     4141 caggacctca ccggatgcct tggggccgac catgaccacc gatgggtact tcatggtcag
     4201 gcgggatccg atgttgccgt cgatccactc catgtgcccc tcggcctcga cccgggcccg
     4261 cttggtgacc aggttgaaga cgttggagga ccagttctgg atggtggtgt aggtgacccg
     4321 ggcggcctcc ttgaccacga tctcgaccac ggcggagtgc agggagtccg acgagtacac
     4381 cggggccgag cagccctcga tgtagtgcac cttcgacccc ttgtcggcga tgatcagcgt
     4441 ccgttcgaac tggcccatgt tctcggcgtt gatgcggaag taggcctgca gcggcatgtc
     4501 gacctcgacg ccgggtggga cgtagatgaa cgaccctccg gaccacaccg aggagttgag
     4561 cgccgagaac ttgttgtcgt tgcgggggat gacggtgccg aagtactgct tgacgatctc
     4621 ggggtgctcg cgcagcgccg tgtccatgtc gcagaagatg acgccctggc gctcgaggtc
     4681 ctcgcggttg cggtggaaca cgacctcgga ctcgtactgc gcggtcacgc cggccaggta
     4741 cttgcgctcc gcctcgggga tgcccagctt ctcgtaggtg gacttgatcg cctcgggcag
     4801 gtcgtcccac tcgtcgacct gcgagtcggt gggcttgatg tagtagtaga tgtcgtcgaa
     4861 gtagatctcc gacatgtcgc caccccagtt gggcatgggt cggcgttcga acgcccgcag
     4921 ggacttgagg cggaagtcga gcatccactg cggctcgccc ttcatccacg acatctcccg
     4981 cacaatcgcc tcgtcgaggc cccgcttggg cttgaagacg tagtcctcct cgtcgctcca
     5041 tcccagggag tacttggaga ggtcgaggtc gaccgacatg tgggctcctc acgcgggtgg
     5101 gcgtctcggg agaacggacg gggggtttct cctacggcga tagtaacaac ggtcggtgtc
     5161 accggacatc ccgacgcggc accggtggac gcgggcccgg gcgtagggtg gtcaccgtga
     5221 ccgtcaccga gggcgaaccg acggctccga ccgcccccga gcctccccct ctgggctggg
     5281 agccgcccgc accgctaccc cggaccaccc gaccgcgatg ggtggcgccg gccgccacgg
     5341 gtgcggccct cgtcgccggg tgcgccgcga tcgcgctgtg ggatcccgga gacgggggcg
     5401 ttgccgtgtg ctggtcgaag gcactgttcg gcatcgactg cccgctgtgc ggtggcctcc
     5461 gcgccaccaa cgccatgatg cggggcgact ggctcgctgc ggcggatcac aacgtgctgg
     5521 tcgccgtcgg gctgcccctg ctcgcggtcc tgtgggccgt gtggctcatc cgctcgatcc
     5581 aggaccggcc gctcgacctg ccccggttcc cccgcgccgc ggtcatcggg ttcggggtgt
     5641 tcctgctggc gttcgcggtg gtccggaacc tggacgtgga cgtcgcctgg atccactggc
     5701 tggcgtccga caccgcctga gcggacaaga gccccgtttc cgccccgaac tcccgttctg
     5761 tagtgcgaga tccacacgtt cgtgtggatc tcgcactaca gaacgagctg gcgctacgga
     5821 acgggttggg ggtcgacgcc ggggatcgtg ggtaccgagg tgaccgacgg cagcgtcgag
     5881 gtcaccgatg gcaccgtggt cgtcggcgtc gagacgcccg gcgccacggt ggtggccccg
     5941 agcggcgctt cggccggaag cgacgtggtc gtctcggtgc ccgggaggct cgtgggcgac
     6001 tcggccggca cggtggtcga gccggtcgcc cagtccggcc acggcgccgc tgcgtcggcg
     6061 aagtccaggc tgaccgtggc cgactcgagg ggcgggaagc cgatcccggc ccccgacacc
     6121 gtcgccccgg tcaccccgat cgccgcctga cggacggtcc ccgcgaagtg gagctgcccc
     6181 gtgcggggat cctccatggc cagcacggtc acggcctggg ccgcgtcgac gcggaccgac
     6241 aggaaagcca ggcgtccgac gtgaccgtcg tcgtaggtga ccgtcgagat cccgatggcg
     6301 aaggcgtggc cctgcgcctc accgacgaag tagtcccggg cgtcgacgca cgccccggtg
     6361 acggaccacg ccgggccgct caccgatgat gacgacatcg aggccgtcac gcggtccccc
     6421 gcacacccct gggccccggc cgaggtcgcg ggcaacacgc cgacagcgac ggtgacggcg
     6481 gtcagggcga tggcgagggt gctcaggtgt cgtctcatgg gtggggtggg ttcgcaatgg
     6541 cgaccgggat cccgatgcgg agccgcgggc ggagcgccac ggctcgagtg tgggactccc
     6601 tctccggaaa gggtagaggg cagatgcccc ttccggtggc gcattcctga gccgaaccgc
     6661 agtgggaggc gatctccgag ttcaggaaga cgccgagtcg gagaagacgt cgacagccgg
     6721 tccgcttcag tccagccggc gcagatgcgt tcggaaccgc tcccgtcgat cgacgtagga
     6781 ctggacgccc agatcgaagt gccccggggt gacggcgtcc tcgcggatga cggcggggat
     6841 ccccagcgcc atcgccaacg gcggcaccac cttgttgttg ccgacgaacg cgcccccgcc
     6901 gacgatggcc tcgcgcccga cggtggcgtt gtgcaggacc ttcgagccga tgccgatcag
     6961 ggccttgtcc tcgacggtgc agccctcgag gtgggcgagg tggccgatcg tgacctcgtt
     7021 gccgaccgtg gtcggccaga agggcgtggt gtgcaggacc gctccgtcct gcaccgatgt
     7081 ccgctccccg atgaagatga agccgtcgtc gccgcggagc actgcgcacg gccacaccga
     7141 ggattcggca ccgatcacca ccgaaccgat gacgacggcg tcgggatgga tgtaggcgct
     7201 ggggtggatc tcgggttcct ggtccccgag ggcgtagatg ggcatgggac tcccgtgctc
     7261 gagtcggacg tggctcagat cttggcgtgg cggccgtagc cgcccttgaa gaacagcagc
     7321 ggaccgacgt cgggccggtg cacgtcgagt gagcggacca gaccctcgac gatcacgtgg
     7381 tcccccccgg gatggatctc atggatctcg cagtcgatgt gagccacggc accgtggatg
     7441 agcggcgatc gcgtgatcgg cgacggatcc cacgagatgc cctggaactt gtcgtcggcc
     7501 ttcgacgcga agcgaccgga ggtctcggtc tggtcctcgg ccagcacgct gaccccgaac
     7561 acgccggtcg aacggatcaa cggccacgtc gaagaggtcc gggagacgca gaagccgacc
     7621 aggggcgggt ccagcgacac cgagaagaag ctgccgatgg cgaggccgac cggccggccc
     7681 tccacctggg aggagatgac cgtcaccccg gtcgggaagt gaccgagcac ctggcggtag
     7741 agcatcgggt cgatctcgat ggggctctct gcgggttctg tcacggcgct gtcgctggtc
     7801 acggaccgaa cctaccggcc caccgcgacc tgcgggaacg cggcgtcggc gccgacaccg
     7861 accgactcct gcccggtcgc acccgacggc tcccgggcac ccgagcgacc gtcgggaacg
     7921 gatctggcca actcgactcg gggcgtcagg gtcacggtga gcccctcgtg ggctgcgatg
     7981 acctcgggga cgccgcggag gtccgcgagc tcgcgggccg agaccagcag ctcgtcgacg
     8041 cggcggtcgt cgtggcccgg gtcgtggtgg aacagggcga gggtgtgcgc cccgctggcg
     8101 gccgccacct cgaccgcgta gtcgacggtg cagtgacccc agtccggtcg ctgggcgaac
     8161 tcggcgtcgt cgtactgggc gtcgtggatc agcaggtcga cgtcccggca cagctcgagc
     8221 accgccggat ccaccgcggt cggccccgcg gtcggctgct ggtggtcgct cacgtaggcc
     8281 accgaccggc catcccgctc gacgcggtac ccgaaggtgg gccccacatg gggcacccac
     8341 gccgcggtga ccgtggcgtc gtcgacccgg aaccggccgg gttcggtgtc gtggaaccgg
     8401 atctggcccg gcaacgcctc gagctcgacc gggaagtagg gcggcgagag gaacccgcgg
     8461 acaacgtccg cgagtggctc accggcctgg accggcgcgt ggaggtccaa ctccgctccg
     8521 gcgctcagca gaggtgcgaa gaaggggatc ccctgcacgt ggtcccaatg caggtgggtg
     8581 agcagcgcgg tgcctcggaa cggttctcca acggctccgt ccatgtctcg tcgggtgagc
     8641 tcggctccgt agacacgcag tcccgtcccc aggtcgagga tgatcggaga ccggcccgga
     8701 ccgtcgagca ccacgcacga ggtgttcccc ccgtagcgcg cgagctctcc tccggagcat
     8761 ggcgtcgatc cgcgcacacc gtagaaggtg acgttcagga cggtccccct ggttgtcggg
     8821 acggtccctg ccaaccgtcc ggacagcaag agtacgggac cccgagggtc acgtgccgtg
     8881 tgctgtgacc gaaatctcgg acggtccggt ctctccccgg ggcaggtcga acgtgtcgtc
     8941 ccggttcgcc acgacgagac cgtcgcgcac cagcccgtcg agcgccgcga cgagtcgctg
     9001 cggatcgacg gtgttcccga cgagcaaacg ctcgagcgag gcccgggatc ggggcccatc
     9061 cgacaggtga tccagcactc gaccgcgcag ctcccgatcg gagccggcga acggagcctg
     9121 gcgccgcgac accccggccg agccctgcgc cggatccgga tcggggaacc cggcgtcccg
     9181 ccaggcgcag tcgaccggac agccgtcgca gtcgggtatc ggtcggcacc tcgtcgctcc
     9241 gaggtccatc agcacctggt tccactccca ggcgcgctca cggggcaccc aggcgtcggc
     9301 gagctcctgg gcggctccgg cggtcaatcc ccgcccggcc cgacgggcga gaacgcgggc
     9361 gatgttggtg tcgaccaccc cgacagcaga cccgccggcg aaggccatga ccgctcgtgc
     9421 ggtgtagggg ccgaccccgg gcagcgccag caggtccggg agggtggagg ggaccgagcc
     9481 accgtgtcga tccacacagg cgcgggccga cagccagagg taccgggccc gtctcgggta
     9541 gccgagtccc ttccagtcga cgagcaggtc gcggagcggg gcctcggccg cagaggcgat
     9601 cgtgggccat cggtcgagga accgctccca tcggggtgcg acccgggcga ccggggtctg
     9661 ctggagcatc acctcgctga cgagcaccgc ccatggatcg cgggtccgac gccacggcag
     9721 gtcccgcagt cggggcatgc cgccgtcgag gactgcctcc tgctccgcgg tcaggtcccg
     9781 ggtcagcgcc acgtccgagc cgcacggacc ccgcacgtgt gcaggaccgt ctcgtggggg
     9841 taggcgaagg tcccggtcag ctcaggatgg ctgtcgagca gatccgctgc ctcttccagc
     9901 aggtcctccc ggggcccttc gtcgagcgcg gcgacgaagc tggtggagcg cagccggtcc
     9961 atcacccctt cgacggtcgt gggcaccggg ttcgggtagc ggcgctcgac cagcggctcg
    10021 aaccggccgc tggactccac cacctcggcc cacggtcgtt cccggtggtc gacgtagggc
    10081 cggccgccgg tgcggtcctc gaccagccga gtcagctggt ccacccactc ggtcgactcg
    10141 tcacgggcgt tccacaccag ggccaggact ccgtcggggc gcagcacacg tgccgcctcg
    10201 gcgagggcgg ccccggcgtc gaaccagtgg aacccctggc cgacggtgat cacgtcgacg
    10261 gacgcgtcga ccacgggcag ggcttcggcg aggccgaggg ccaccgggac cgcaggtagg
    10321 gacagacgga gctgggcggc catcgcgggc aggggctcga cggcccacag gtcgagtccc
    10381 gctcggagca gttggccggt gaggatcccg gtgccggccg cgacgtcgag gacgcgtcca
    10441 ccccggggcg ccagatcctt gatcgctccg atcgcggggc gggcgtaggt cgggcggatg
    10501 cgggcgtagg tggacgccgc ggccgagaag ccgccggcgg cgacggggtt gacgggcacg
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggtcatcg gcgccgcgat
    10681 catggtcctg ccgatgttcg gcgcccaccg gttgcagatc ggggccgtca tcctgtcggc
    10741 cgcgaccgtc tccaacatgg ccctcttccg acgggcacgc cggggcggga gcgtgccttc
    10801 ggccgtcgcg atcgccgatc tagcgctcgc gtgtgtcgtc gccacgatcg ttcccgcctc
    10861 ctacggggtg gtggtcgtca tcctggtgtc gagcgccggg ctctacgtct tctggttcgg
    10921 cccgcgcacc gtgtggcggc tcatgccgtt caccgccgcg gccctggctg cgatcggcat
    10981 ccgcagcggt tcgaactact gggtgccgtt cttcgtcgcc tggtgcgtca ccaccacgtt
    11041 ctcgacgatc atcttcggtc acctcgcccg gacgctcgcc gctcagcgcg accggttcga
    11101 cgagctggtc aacggcatcg atgccgcggt gtgggaggcg ccggggcccg ccggacccgc
    11161 cgacttcctg agcaagcacg tggtcgacct gctcggcttc gagcgcgacc gggtgctcga
    11221 ccccgagttc ttcctgtccc gggtccaccc cgaggaccgt tcggtgttcg tcgagggaca
    11281 gcagctggtc gccggcggac aggacgtcga ggtccacttc cgggtcaccg acgcgtgggg
    11341 gcgtgtccgc cggctgcaga accgggtgcg cgtcaccctc gacggcaccg gccgggtcgt
    11401 ccgacgccgt ggcatcgtcg tcgacgagac cgcccgatgg gaagccgagg ccaccgtccg
    11461 gcagtactcg gacttcgtcg agggcatacc gatcgccctc gtcgtcatcc agatgaccga
    11521 cccgacggat cgctcgagct tcgtgctcag ggccgccaac cccgccgcca acgccctcgc
    11581 cgacctcgac gaccgggcca tcggacaccg gatcgccgac ctcatctccg tcaccgacgc
    11641 cttcctcggc gacatgagcg acgtggtcgc gagcggcgaa ccgcacgacg agccgttcgt
    11701 ccagctcgcc ggagtcgacg agatcttctc gatgcgggcc gtgccgctgc cggaccggtg
    11761 cgtcgggatc tcgctcgagg acatcaccaa gcgggcccgc accgccgagg cgttccgcca
    11821 ccaggcgacc cacgacgcgt tgaccgggct cccgaaccgg gccctgctgc acgagcggct
    11881 gacccgcgcc ctcatcgagg ccgaccggac gggggccccg gtcgggctcc tgatggtcga
    11941 cctcgaccag ttcaaggacg tcaacgacgc cctcggccac gagtacggcg atgcgctgct
    12001 gcgcgagatc gcccgccgga tgtcggaccg actgcgcaac tgcgacacga tcgcccgcct
    12061 gggcggcgac gagttcgccg tcctgttgag caccgacgcg tcgatgtccg gcgcggagga
    12121 ggttgcacgg cgcctcaacg agctggccac cgaaccggtc caggtcggcc agtaccggtt
    12181 gcaggtcagc gcgagcgtgg gcatcgccct ggctcccgag cacggcaacg acgccgagac
    12241 tctgatgcgg cgcgccgacg gggcgatgta cgacgccaag caggccggcg ggggtcacgt
    12301 ggtctactgc gccgcccagg agctcgctgc ggtcaagcga ctcgagctgc tggccgacct
    12361 gcgcggcgac ggcttcggtg agcagatgga ggtccacttc cagccccgcg tccagctcag
    12421 ctccggcgcg acggtgggga tcgaggccct ggtgcgctgg cgccaccccc gtcacgggct
    12481 gctcgcaccg gcggagttca tcgagctggc cgaggtgtcg ggcgcgatcc gcggcatcac
    12541 ccaggcggtc acgtcccgga cctgggacgc cctcaccgag ttggagcgcc acggcatccg
    12601 ctcggccagc gtgaacctgt cgacccgcaa cctgttcgac ccggcatggg tggagtggat
    12661 gtgcgagatg gccgagaccc gcggcatcgg cgagggccgg ctctggatgg agctgaccga
    12721 gggccagctg atggacgatc cccgccagag caccgaggtg atcgaacggc tccaccggat
    12781 cggcatccgc ttcagcatcg acgacttcgg cagcggtgcg tcgtcgctgg cgatgatccg
    12841 cgacctgccc gtcgacgagg tgaagatcga ccgccgcttc gtcgacgagc tcctcgacgg
    12901 cgacgaccgg atcacccgtt cgatcgtcga cctcgcgcac cacctgggcc tgagcgtgac
    12961 cgccgaaggg gtcgcggacc cgcggctgat cgagacgctc cgctcgatcg gctgcgacac
    13021 ggtccagggc ttcgccctgg cgcgaccgat gccgctcgac gagctgcgga cccacctcgt
    13081 ccgcgagcag gccgagggct tggaggacgc ccgttaccgg ccggtaacct gaccgccatg
    13141 acgagcgctg acctcccctc ccccgacctc gatgccgtgg ccgcggccct ggacgtggcc
    13201 caggcggtca tcgacggcgg catcgcccga ctggtcgacg gcgggatcga ccagaaccag
    13261 gtcctggcct acgacgtcgc ccatgctgcc gccggcatcg gagcgtcgcg gagcctcctg
    13321 gcctacggag cccagggaga cgtccaggcg cggattgcgt gcgtgttcgc cgccgaggcg
    13381 ctggccgacc tggccgggaa ggtcttcgga cgcgaagcgg actggggtgc ggccccggga
    13441 gcgctggacg gcgcccgcca ggccaccgcc gcgtaccggt cccccgcctt ccaggcgtcg
    13501 ctcgccggca ccgacggccc gcgcggcctg gccgacgact tcgagatggt gcaggacacc
    13561 ttccgccggt tcgccgagga caagatcgcc ccgatcgccg agcacatcca ccgccacaac
    13621 gacgacatcc ccgaggagat catcaccggg ctcgccgaga tgggggcgtt cggactctcg
    13681 atctccgagg agtacggggg cttcgccacc ggcaccgagt cggactacat cgccatgatc
    13741 gtcgccaccg aggagctctc gcggggctcg ctcggggcgg gcggctcgct gatcacccgc
    13801 cccgagatcc tggcccgggc gctcgagacc ggcggaaccg aggagcagaa gcagcactgg
    13861 ttgccgcagc tcgcctcggc cgaggtcatg aacgcggtcg cggtgaccga gcccgactac
    13921 ggctccgacg tcgccggggt caaggtcacc gccgcctccg tcgagggtgg ctggctgatc
    13981 aacggcgtca agacctggtg caccttcgga gcccgcgccg acgtgctcat gctgctcgcc
    14041 cgcaccgacc ccgaccgcag caggacccac cggggtctgt ccatgttcat cgtccccaag
    14101 gagcgcggcg acggacacgg cttcacccag acccaggaca acgcccacgg tggcggcaag
    14161 atggagggcc ggccgatcga cacgatcggc taccggggaa tgcactccta tgagatcgct
    14221 ctcgagaact ggttcgtgcc ctcggagaac ctgatcggcg gcgaggccgg cctcggccgc
    14281 gggttctact accagatggc cgggttcgag aacggacggc tgcagaccgc ggcccgcgcc
    14341 gtcggcgtga tgcaggccgc gctcgaggag gcccgccagt acgccgagga ccgcaaggtg
    14401 ttcggccagt ccgtgggcga ctaccagctg aacgctgcca agctcggccg catggcggcc
    14461 accgtccagg gcgcccggca gttctcctac gacatcgccc gccaggtcgc cgggggcgag
    14521 ggccgcctcg aggccgccct gctcaaggcg tacgtgtgca aggccgccga gtgggtgacc
    14581 cgtgaggcca tgcagatcca cggcggcatg ggctacgccg aggagtacac cgtgagccgc
    14641 ctgtgggtgg atgcccgcgt cctgtcgatc ttcgagggag cggacgaggt gctgtgtctg
    14701 cgggtgatcg ccaagcagct ggtcgaacgt gccgcggccg actgaccgtc gcggactgcg
    14761 tcccggtccg ccggggcggt cgtctcagat cctgaccgcc gtgatgatca cggcggcgaa
    14821 cgcgacgagc gttccgatgt tgatcaggac cgtccgacgc atctcgtcgc gcagctcgct
    14881 gcgcatcgcc cgcatctcgc tgcgcagcga tcccacctcg gactgcaact ccgaacgcaa
    14941 cgatcccacc tcggactgca gctccgaacg caacgatccc acctcggact gcaactccga
    15001 acgcagcgat cccacctcgg actgcagctc cgaacggaac tccccacgca gctcctgcac
    15061 ttcgccgcgc aacgcaccca cctcggactg cagctccgaa cgcagcgact ccgacagccg
    15121 catctccaac tctgcgagct cggcccggag gaattccttc gtcaccgact cctcgacgtc
    15181 acgagcgggg aagtacgaca gcatctcttc caccgcctcc tcgtcgatga cgtggctcag
    15241 ccccagatag agggccgacc tgctcttctc tgtcaacgcc atgtcttcca cctgccatcg
    15301 ccagctgtcg ctcggtgacg atcaccagcg acggagggga agtggacaca tcgtaagcga
    15361 taccccgaca gccacaacga gcctcgacgg gctcggaaca gggatcgaac gaacggaccc
    15421 ggttcagatc ctgaccatga cgtgcttgat ctcggtgtag tgctcgagcg cgtagatcga
    15481 catgtccttg ccgtaccccg actgcttgta gccgccgtgg ggcatctctg agacgatcgg
    15541 gatgtggtcg ttgacccaga cggtcccgaa ctgcagtcgc ttggccatcc gcagcgctcg
    15601 agcaacgtcg ttggtccaca ccgacgaggc caggccgtag tccacgccgt tggcccactg
    15661 caacgcctgg gcctcgtcgt cgaaccgctg caccgtcacc accgggccga acacctcgcg
    15721 ttggacgatc tcggagctct gctcgggccc caccaccacc gacggttggt agaagaagcc
    15781 ctctcggtcc ccgaccgctc caccgacggt gacctcggcc cccgccgcgg ctgcccgctc
    15841 gaccatgccg gcgaccttgg cctgctgctc ggccgagatg accgggccca cggcgacgtc
    15901 gggatccgag gggtcacccc agcgcagcgc cgcgaccgag tcggtcagcc ccgacaccac
    15961 gtcgtcgaac acccgaggac cggcgatcac ccggcacggg gcggtgcagt cctgacctga
    16021 gttgtagtag ctcatgtcgg tcaggcactc gaccaccgcg gcgggatcgg cgtcgtcgaa
    16081 cacgatcacc ggggctttgc cgcccagctc caggtgcacc cgcttgaggg tctcggccgc
    16141 gttgcgggcg atcagcttgc cggtcgacac gtcgccggtg atcgacacca tcgccacgtc
    16201 ggggtgcgcc acgagcgccg cgccggcgga ctcgccctgg cccagcacga cgttgaacac
    16261 acccggcggg aagatgtcag ccgccagctc ggccagtcgg atcgcggtga gtggggtgag
    16321 ctccgagggc ttcaacacca cggtgttgcc ggtggcgagc gcgggaccga tcttccatgt
    16381 cgccatgttg agcgggtagt tccacggggc gatcgaggcc acgaccccga gcgggtcgcg
    16441 tcgcacgaag ctggtgtggt cctcgaggta ctcccccgcc gcacgtccct ccaggaagcg
    16501 ggctgccgcg gcgaagaagc ggaagttgtc gacggtgagg tcgaactcga agtcgatgat
    16561 cgacacgggc ttgccgacgt tgctcgactc cagcgccttc aggttctcga ggtcgccctc
    16621 gatcgcgtcg gcgagcgccg tgatcatctc gaagcgggcg cgcggggtgg tctcggacca
    16681 ggtctcgaac gcagcccggg cgccggcgac cgcagcgtgc acatcggcgg cacccgacga
    16741 ggcgacggtg gcgatgacct cgcccgtcgc gggggccacc acgtcgtcgg tggcgccgga
    16801 cagggcggcg cggaactcgc cgttgatgaa gttgccggtc tgcatcggct cagtctgccc
    16861 cgctccgggc gcacgtgtcg tggttccctg caccacggat cggtcgctcc gggccccatc
    16921 gccgccgacc ggacggggcc gacaccgatc cccgcagtca gactcggtcg aggcggacgc
    16981 agcaccggcc gggctcaggg gcaagggtcg cacggtaggt cggcgacgct ccgatgccgt
    17041 cgatcacccc tcggagcacg tccaggttca tcccgcagat caggtcgcgg tgctcctcgg
    17101 ccaaccggtg gaacgggcag ttgtcgagga ccacgtcgtc gtcaccgaca cagggctggt
    17161 acccgtgctc gatcaaggcc tcgatcaccg cgtgccagcc ggtcaggtca tccgcgtcgg
    17221 cgaccgatcg gccgatctcc agacccaggg ctcgagcgcc ccgagtgagc gcctccccaa
    17281 ccggctcacc gtgttcggtt gccgcggcga ccgcatcggc gagcagtcgt cccgccaggt
    17341 cgtagcgtcg atcagggacc gaagctccca cctcgtcgac ggtgaggcgg taccgcttcg
    17401 ccggccgacc ggccccgggc ccggtacggc cggccgcccg ctcgtagtcg acctcgacga
    17461 cgccgagcgc ggccagcttg tccaggtgga acgccgcgac ggaccgcggc accccgagcg
    17521 ccgcggccgc gtcgtcgcgt ccgacccagc ccgcccgttc gacgagcagg tcgtacagac
    17581 gacgacggag cggctgctcg agtgcggcga ccgcggcgat ccgggcgtca gggtgtcgca
    17641 gtgcgtccac gggaacactc taaaacgcaa tctcattgat gaaatcggaa catctccgca
    17701 cccacccgag ttgtcctcag agagcgggac agtccgaccc cacacccgac cagggaggca
    17761 cccgatgcgg aagttctctg cccgacccac cctcaccctg aagggtcgca ccttcaaggg
    17821 ccctcgaggc tggtccggga agccgtccca cccgccgctc accgacatcc ccatcggcgc
    17881 ctacctgctc gttgcgggat tcgacgtggt ctccgcgatc gcgggcgaca gccacggatg
    17941 ggccggcgac ctgtggcgga ccggaacgtg gatcctggcc gccggtctgg cggtatcggt
    18001 gctggccgcc ctcacgggac tggccgacgc ccgatcgtcg tcagaagccg ggacccaggc
    18061 tcgacggacg atcaacaccc acgccacctt catggtcacc gccacactcg tcgccctggc
    18121 cgacctcgtc tggcgcctcg cggttcatga caccgcgctg gtcacaccgg tcgggatcgt
    18181 cgtgctgtcg gtgatcgtcg ccggactggt caccgtcggc gccacgttcg gcggctcgct
    18241 ggtcttcgag tacggcttca acgtcgagac cgccggcgac cacccggtgt ggcaccgctc
    18301 ggagaccgac gtactccccg gccaggacca ctgacccggg gtagtccctt cgagctcagc
    18361 tgtccgacag ggcggcgatg gcgtccgcga tggcgatcgc ccgacgtgcg ttgtcgacgt
    18421 gcaggttctc gatcatccga ccgtcgacgg tgatgacgcc cttgccctcg gccaggccct
    18481 gctcgaaggc ttcgatcacc cgccgcgagt actcgacctc gtccgccgac ggcgagaacg
    18541 ccgcgttggc cggttcgacc tgcgtcgggt gcacgagcgt cttgccgtcg aagcccatct
    18601 cggctccctg ggcggcctcg accgcgaatc cgtcggggtc gcggacgtcg ttgtagacgc
    18661 cgtcgaggat caccttgtcg gcgaaccgtg ccgcgttgag gcaccgggcc agcccccaca
    18721 gcagcggcgc gcggccgggc accagcccgg cccgcagctc cttggccagg tcgttggtgc
    18781 ccatcaccag caccgccagc cgctcagagg aggtcgcgat ctcaacggcg gcctcgaccg
    18841 cggccggggt ctcgagcatg gcccagatcc gggtgtggtc gggaacgccc gcggcctcga
    18901 tgatgcgctc cacctcggcg acgtccgcgg ccgagttcac cttgggcacg acgatcccgt
    18961 cggggcccgc ctgcgcggca gaccgcaggt cgtcgacggc ccactcggtg tcgagcgagt
    19021 tgacccggat ggtcagctcc ctcgacccgt actcgccgga ctgcgcggcg gccgcgacct
    19081 tctcgcgtgc gacggccttc atgtcgggtg cgacggagtc ctcggtgtcg aagatgatcg
    19141 cgtcggtggg aagggacttg gccttctcga gcgccttgtc attggcgccg ggcatgtaca
    19201 gcgcgctgcg gcgcgggcgg aggtcgtcgg tcatctcgtg cgtccttcgt gtgctcagaa
    19261 gccgtagcgc tcggccaggg acgggtcccg ctcggcgagc tggcgggcca ggttgaccac
    19321 gacgtggcac tgcttgaccg acgcgtcgtc ctccatcttg ccgtcgagca tgatcgcccc
    19381 ggttccgtcg cccatcgcct cgatcacccg ctgggcgtga gcgacgtcgg ccggatcggg
    19441 cgagaagacc ttcttggcga tgtcgatctg caccgggtgc aacgaccagg cgcccacgca
    19501 gccgagcaga taggcgttgc ggaactggtc ctcgcaggcg acggtgtcct tgatgtcgcc
    19561 gaacggaccg tagaacggca ggattccggc cgccgcgcag gcgtcgacca tgcgggcgat
    19621 cgtgtagtgc cacaggtcct gctggtagct ggtgcgctcg gctccgtagg ccgggccgtc
    19681 gtcaccctgc ggcgggtcct ggcggaccag gtagccgggg tggccgccgc cgacgcgggt
    19741 ggtcttcatc cgacggctcg cggccaggtc ggccggaccg agcgacaggc cctgcatgcg
    19801 cggcgaggcg ttgcagatct cctcgatgtt gttcatgccc cgcgccgttt cgaggatggc
    19861 gtgcaccatc agcggctcgg tcagccctgc cttcgcctcg agctgggcga ggagccggtc
    19921 gacgtagtgg atgtcctcag gaccctccac cttggggatc atgatgacgt ccagcttgtc
    19981 gccgacctcg ccgacgaggg tgacgaggtc gtcgagcacc cacggcgaat ccaggctgtt
    20041 gacccgggtc cacagctggg tggagccgaa gtccatctcc ttggcgacct tgaccagacc
    20101 ggcccgtgcc gcctccttgg cgtccatcgg gatggcgtcc tcgaggttgc cgagcaggat
    20161 gtcggtggtc ggggcgatct cggggagctt ggcggtcagc ttctcgaggt gcggcgggaa
    20221 gaagtggatc atccgcgacg gcgggaacgg gatctcccga cggggttcgg gagcgccgac
    20281 ggcgagcggc gcgaagaagt ccttggggct gcgcatgacg ccgacgctag ctgcccgttc
    20341 tgttcgttgg ttcacaacgc ctgcgttgtg aaccaacgaa cagaacggga cgcggtcagg
    20401 agtcgaagcc gaggccgagt cgatcgagca gccgcaacca cggcccgcgt cgaccgccgt
    20461 cggcatcggc gcgagcgatc gcccgcctgg tgagctggat gcccgcggtc cggatcggct
    20521 ccggagggaa cgggatcggc gctcgacgaa ccagatcgag gtcgaggtcc ggcgcctccg
    20581 gagcgaacag catctccacg cacacccgac caccgaagcg tgaggcggcc acccccagac
    20641 cggtgtagcc gacggcgtag gcgactcgac cgtcgagcgc ggttccgaac ccgaccgaga
    20701 agcgcgtcga cgtgtcgatc acgccggccc aacggtgact gaagcggatc ccctcgagtt
    20761 gggggaaggt ccgcaggaag ttgccggcca gcgtgcggaa ggtctcggga cggtcctcga
    20821 agcgctcgtc ggtgcggccc cagttgtgga acaccgcgtc gtagccgccc cagaggatcc
    20881 ggccgtcgtc ggtgagccgg tagtagtgga actggttggt ggtgtcggag atcccctggc
    20941 ggccgcgcca tccgatcgag gcgcgctgct gctcggtcag gggttcggtg gccagcacgt
    21001 ggtcgtacac cggcacgatc cgacggttga tggcccgtac cggcgaccgc tccgcgttgg
    21061 tgcccagcac gacggcctgg gcccggatcc gcccccgatc ggtgcgcacc tcgacgccgg
    21121 cgccatggcg gacgagcccg gtcatggtcg taccctcggc gatgcgcccg ccggcccgtc
    21181 gcaccgcgtc ggccagaccc catccgagcc gtgccgggtc gaccagcgcc tcaccggacc
    21241 gggtccagga cccggagagg taggtcggcg agtcgaccag ggcccggatc ccgtccgcgt
    21301 cgaacagctc gacgtcctcg ccgtgcatca ccgcctggtc ggcgtcgcgg cgcacgaccg
    21361 gcacgcacca cggttcggtt gccaccgcca gcgctccgac caactccagt cgggcatcga
    21421 tctcatggcg tcgcacgtcg gcgaccaggc cgtcgaagtt ggtccgtccg gccgccagga
    21481 tgcggtcgat gtcggagccg aatcggtcga ggccgttggc cagcccgtgg gtgagcgaag
    21541 ccgagacgaa tccgccgttg cgaccggacg cccgagccgc caccggaccg gagtcgatga
    21601 ccagcaccga ccgcgaaggc tcggactcca gcgagtgcag agcggcccac aggccggtgt
    21661 agccggcgcc gaccacgacc agatcggctc gctcgtcgcc gtcgagcgga gccccaaccg
    21721 cgggacggtc gtcggtgtcg gtccacaaca ccgacagctg cgagtcggcg agcgcagcgt
    21781 cgaccagcga ggcgtcgatc accgccgcag tggcggtagt cgagcggagc ggtcgaaccg
    21841 gcgacatcga ccgatcctgt ccggcccggt cggagcggtc aacgccacta ccgttccgag
    21901 catgcgtgcc gtgatgtgcc gggagctggt cgaccccgag gagctgaggg tcgaggagct
    21961 cgacaacgtc ccctgcggac cggaccgggt gagggtccgc atctgggcat cgggggtcaa
    22021 ctacgtcgac gccctcttcg tccaggggca gtaccagatc aagccaccgt tgccgttcat
    22081 cccgggctcc gagatcgcgg gtgagctcac cgaggtcgga ccggaggtcg agcggtggga
    22141 ggtcggcgac cgggtcatgg catcggtcgg actgggtggg ttcgcggacg agatcgtgct
    22201 tcgggccgac cagctcacgc ggatccccga ccgtctgagc ttcggtcagg ccgcgaccat
    22261 gggtcagtcg tactgcacgg cctggttcac cctcacacgc cggaccgtgc tccgccccga
    22321 ggactgggtg gtgatcttcg gcgcggccgg cggcgtcggg ctggcgatgc tcgacgtcgc
    22381 acggagcttc ggcgcccaca ccgtcgcggt cgcgtcgagc cccgagaagc tgcagttggc
    22441 gatcgagcgc aacgcgaacg ccgtcatcga ctcctcggac gccggatccg atggtgccac
    22501 gatgcgcgcc cggatccgtg aggtgaccga cggcggggcc gacatcgtga tcgacccggt
    22561 cggcggcacc ctgaccgagg agggcctccg cggcctgcgc gagggaggcc ggctgatggt
    22621 catcggcttc gcgtccggat caatccccac cctgccggcc aaccaggtgc tcctgcgcaa
    22681 ccggtcggtg ctcggcgtcg actggggcgc gtgggccttg ggtcaccccg cggagaacgc
    22741 cgcgctcctg ggcgaggtcc tcgcccaggt cgacgccggc gccctcgagc cggtcgaacc
    22801 gacgatggtc cccctggaag acgcgggtac cgccttgcgg gacctgatcg agcgacgggt
    22861 cgtcggcaag gtcgcgctgg agtcacgcgc ccgctgagcg ctcccgcccc ctcccccact
    22921 ccaccccaag cccgttctgt tcgttggatc acaacactcg tgttgtgatc caacgaacag
    22981 aacggggccg ggcgggcggg cgaaggggag cggggaacgg gtgtgggggt caggacgcgg
    23041 cggtggggtc ctgccctgcg gcgagagcgt cgacggccgg gccgaacacg tccatcggga
    23101 tctcgcccga tccgcgggcg acgaccttgc cctggccgtc gatcatcacg aagtagggga
    23161 agccgctgag gccgtagtcg gacgcggccg ccgactggtc gtcgtcgagg acgaccttgg
    23221 gcgtcacccc ctccttctcg atccagtccg acaccgggta gttgccccgc tcgggggcca
    23281 ccccggtgct gacgtagacg atctcgacgc ccttcggcag gttcccgtcg tcgatccact
    23341 gctggatcgc ggggacctcc ttctggcagt gggggcacca gtgggccacg aagacgatga
    23401 ccctgggggt gccgtcaccg gtgtcgacct tcaccggggt tccgtcgaag gacgagccgt
    23461 cgatgctcgg ggcgggcatt ccgatcgcct tgtcggtcga gacatcggtg aagtagctgc
    23521 cgggtgccat ctccggcatg gacggcagcg gctccccggt gacggtgatg tcagcggtct
    23581 cctgctcggc gttcgcggcg gccgcggcgc cggttcccgc cgcggcaccc gagtcggtgt
    23641 catcggaacg ggtggcgagg atggcgacga gggcgcagat cgccaccacg accaccgtgc
    23701 cgccgatgac catcgggatg gtccggcggc gacggtcctg cgcgtggctg agcttggagt
    23761 acggatccgc gccgccgtgg gggtcgcgct tgtgactgga gccggtcatg ccggcacctc
    23821 ctgggggtcg ggcccggcct cgcgtcgcag cgcggatcgg gcgatgagca tgagggtgca
    23881 gatcgtgatg aagccgaccc aggccatgcc gggcagcgac aggaagtgga actcccagat
    23941 ccacagcgtg gagcaggacg cctcgaggct gcacgcggtg gacacggact ccgggaagcg
    24001 ctcgtgcagg tagtggtagg tgctgaccgc ggccccgatg agggcgatcg gcaggacgta
    24061 gcgccacacg gccttgtccc gggcgaccac ggcgacggcc agcacgacca cgagcgggta
    24121 catggcgatg cgctggtacc agcacaggtc gcacggcacg aagtggcgca cctcggacat
    24181 gtagaggctg ccgagcgtgc acgtcgccgc gactccgagt gccaacagga gggaggcgtc
    24241 cgacgcggcg tcgcgccacg cggccagacc cgggcccagc gtccgcgtgc gggcgaggag
    24301 cgcaaccacg gcggacgcga cgacgaaggc cagggccagc acggcgagca tcgccaggaa
    24361 caggctgacc gtgtcgttcg acatgtccgg tagagggtaa cggctgcggc attcctcacc
    24421 ggcctaccgg cgaccggtgt gacctatggc tgagcggatg tccaggatcg acggtgcgaa
    24481 cgcgccggcc tcgacggcga cgtggtgacc tcggggcccg tcggtgcgac ctgtagggtg
    24541 cccgccatgt ccgtctacga caccgagttg accgccctcg acggcacccc gggggtgctg
    24601 aacgaccagg acggaaaggt gaccctcctg gtcaacgtcg cctccaagtg cggcctcacc
    24661 ccccagtaca cccagttgga ggagctccac gagcgctact ccgaccgcgg gttcaccgtc
    24721 gtcggggtgc cgtgcaacca gttcatggag caggagccgg gctcaccgga ggagatcgcc
    24781 acgttctgct ccgccaccta cggcgtgacg ttccccctga ccgagaaggt cgaggtcaac
    24841 ggcgacggac gccacccgct gtacgtcgag ctgaccgcgg tggccgacga ggacgacggc
    24901 tcggtgggcg acatccgttg gaacttcgag aagttcctga tcgggcgcga cgggaaggtg
    24961 ctcgcgcgct tcaacccgac ggtcgtaccc gacgacccga aggtgaccgg cgccatcgag
    25021 gccgctctct gatcggagca cacccggtgt tcagggaacg agcaccgttc gaagtccacg
    25081 cccggcggcc aggtcatcga gcgcctcgtt gacctcggtg agcggccgac gagcggtgat
    25141 catggcgtcg aggtcgagtc gacccgactg ccacaggtcg accagacggg ggatgtcacg
    25201 cggagcgtga caggatccga gcaacgatcc cacgagccgc ttctcggtga acatcgtcag
    25261 ggcgacgttg accgacgcgt cctgctcggg cggcggggcg ccgaccatga ccacggtccc
    25321 gcggctgcgc accgcctgga gtcccgcctc gaccagcgtc gccgagccga cggcgtcgaa
    25381 cgcgacgtcg gcgccgatcc cggtgatctc catcgtccgg ccgaccacgt cggtctcgtc
    25441 gggatcgatc acgtcggtcg caccgaagcg catggccagc tcccgacgtg actccgaggg
    25501 gtccgacacg acgatgcgcg cggctccggc gatcgccgcg ccctgcacga tggagatgcc
    25561 gatcccgccc gcacccatga ccaggacgtg gtcaccgggc tgcaccgccg cggtgttgat
    25621 cgcggctccg accccggtct gcacggcgca gccgatcacg caggcgacgt ccagcggaac
    25681 gtcgaccggg atggcgacgg cccccgaggc ggcgacgatg acctcctcgg cccaggcgcc
    25741 gaggcccaga ccgcggtaca ccacatcgga tcgccccccg gcaccggagc cgaccgggcg
    25801 tgagaggtgg gtggtgccgt cggggaacat cggcatggcg atggagatcg acccggtgca
    25861 caccgacggc tccccccgcc ggcagtattc gcatcgaccg cacgccgggt tcggactgag
    25921 caccacgtga tcgcccaccg ccagggagtc gaccccgggc ccgagctcga ccacctcgcc
    25981 ggcggcttcg tggccgggga tggtcggccc gggcaacggg atgtggccct gcaccagcga
    26041 caggtccgag tggcacaccc cgcaggcggc gatgcgcacc cgtacctgac cgacccccgg
    26101 accttggatg tcgatgtcgt cgaccacgtc gagggggcgt ccgacctcgg tgaacagcgc
    26161 tgctcgcatc ccgggaccct acggcgccga cgaacagccg gctggcccga cgaggaaccg
    26221 tcgggtgatc aggaagacac cgcagccggc gcgagctccc gcgggcggcg gtggcgccgg
    26281 aaccggccca ccagcgtggc cagctcggcc aggacgagca gcggcagggt cgagaccacg
    26341 acgaccgccc actgctcgcc ggtcagcgag gtggtgccga agacgttctg caggaacggg
    26401 atgaccacga tcagcacctg ggcggcgacc acgccgccga cggcgtacca gaacgtccgg
    26461 ttggtgacgc tgaaccggtt gaacaccgaa cggtcaccgg agcggacgac gagggcgttg
    26521 accatctgca ggaagacgaa cgacgtgaac gccatcgtga acgccgggtc gtcctcgggg
    26581 gccgtgccgg gggcggagtc cgaccagccg atgaggatgg ccatgatcgt caccgtcatc
    26641 accgcggcga tcagcaggat cggcaccagt cgggcaccgg agaggatcct ctcctcccgg
    26701 tcccggggag gccggtccat cacgccgggt cgcggcgggt ccatcccgag gctcatcgcc
    26761 ggtgggccgt cggcgaggat gttcacgaag aggatctgga tcgggttgaa cggcgccggg
    26821 tagccggcca ggcgggcggt caggatcgtg gcgatcgccg cgatgttcgt cgtcagctgg
    26881 aagcggacga aggtgacgat gttctcgtag atcgcccgac cgcgctccac tgccttgacg
    26941 atcgtggcga agttgtcgtc ggtgaggatc atgtcaccgg cttccttggt gacctcggtc
    27001 ccggtgatcc ccatcgcgac acctatctcg gcctgtttga gcgacggcgc gtcgttcact
    27061 ccgtcgccgg tcatcgccac gacctcgccg ttggactgga gcgccttgac cacccggacc
    27121 ttgtgctcgg gcgacacccg tgcgcagacg ccgatctcct cgatctgcgc cgccaggtcg
    27181 tcgtcgctca tgtcgttgag ctcggcgccg gtgatcaccc gaccggggat gccgagctcg
    27241 gctgcgatcg ccccggctgt cgtggcgtga tcccccgtga tcatccgcac cccgatcccg
    27301 gcgtcccggc actccgcgac cgccgccacc gcctcgggtc gggcgggatc gaggatcccg
    27361 accagggtct cgagggtgag accgtcgatc tcggccgaca ggtcgagctc ctcggcgtcg
    27421 gagccggtcg ggtccgtccc ggcgacggtg ggcacccggt cgcccaggac cttggtggcg
    27481 accgcgagca ctcgcaaccc ctcgaccccg agcgcgtcgt tgtcgtccat tgcccgctgg
    27541 cggccgtcgc cgtcgagcgg cacgaccccg tcggccagcc gccgggccgt gcaccggctc
    27601 agcacgacgt cgggagcgcc cttgaccacc agcagagagc cctggtcgtc catcgggtgc
    27661 agcgtcgcca tgaacttgac cgcggagtcg aacgggacct cggcccgccg aggcacctcg
    27721 gagcggaccg cgtcgggatc cagtccgatc ttgcgggcgg cgacgaccag ggcaccctcg
    27781 gtcgggtcgc cgacgagctg ggcctcaccg tagtcgccgg tgcggacgtg ggcgtcgttg
    27841 cacagcactg cggtggtcag agcccaccgc aacgcgtcgt ccgtggtcgg gtccggctcc
    27901 ggggatccgg cgatcacgga gacgtcgcct ccgtcgccgt atcccagccc cgagaacccg
    27961 aacgtccgcc cagccgccac gacccgggtg gcggtcatct ggttcagcgt gagggtgccg
    28021 gtcttgtccg agcagatcgc ggtggtcgag ccgagggtct cgaccgacgc cagtcgcttg
    28081 acgatggccc gctccgccgc catccgcgac acgccgatgg cgagggtgac ggtgaccacc
    28141 gccggcaggc cctcggggat ggcggcgacc gccagcgcca cggcaccgag gacggcgtca
    28201 ccgatctcgt cgccctggga cagcccgacc aggaagacgg cgagcaccgc tactccggcg
    28261 atcatggcca ggcgcttgcc gaggcgatcg agctgctcct gcagcggcgt ctggctgtcc
    28321 tcggcggtgt tgagcaggcc ggccagccga cccacctcgg tggacatgcc ggtggcggtc
    28381 accaccatct cggcccgacc cctggtgagg gtggtgttca tgtagccgca gttggcgcgc
    28441 tcggcgaggg ggacgtcgcc gccgctgtcc gtgtcgacct cgatcacgtc ggaggtcttg
    28501 tcgacaggaa cggattcacc ggtcagcgtc gactcatcga ccgacaggga gcgggcgacc
    28561 aggaaccggc catcggcggg gacccggtcc ccggcctcca tgacgaccac gtcgccggga
    28621 accagctccg acgccgggag ctccagcatc ccgccgtcac gacgaactct cacggtgacc
    28681 tcgagcatct gctggagggc ggccagggcg tcgtcggcct tcgactcctg cacgtagccc
    28741 aggacggcgt tgatcaacag gacgatcccg atgacgatcg ggtcctt
//
LOCUS       DAOQOD010000015        28071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_548_length_28071_cov_12.400271, whole genome shotgun sequence.
ACCESSION   DAOQOD010000015 DAOQOD010000000
VERSION     DAOQOD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 28071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28071
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_28071_cov_12.400271"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLZ72_03065"
     CDS             <1..427
                     /locus_tag="PLZ72_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35311.1"
                     /translation="ADARAALRKGADLADLEESVQGSRLALTTERCVPRQYLPEALRT
                     PVASARIGALVGPVPGVFPGDDAVVWFRVESAKVVPYSDAGDALAELVRLIAQQGVES
                     WLNLKANALVDIDPRYGSQVEVGQQGLTVLAPATPVGTP"
     gene            438..1802
                     /locus_tag="PLZ72_03070"
     CDS             438..1802
                     /locus_tag="PLZ72_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNA35312.1"
                     /translation="MLHLVGLGPAGPDLVTVGTRDLLDGGRPVFLRTRRHPAASVVPD
                     GTVDFDDVYEAADRIEDVYREIVERLVDAEARHSSAVYAVPGSPVVAEHTVELLVADP
                     RVEVVVHPALSFLDLTWARLGVDPVERGVRLVDGHRFDSEAAGERGPLLVGQCDSLFV
                     LSDIKLAVEDPPREPVVVLQRLGLPDELVAEVAWEDLDRVVSPDHLTSLWIPRLAAPV
                     ASDVARFEALLRRLRVSDPWKAEQTHDSLKRFLLEESYEVLEAIDAYDPESGAGADEL
                     TSELGDLLYQVVFHAALGAEAGWFDLSDVARAIHDKLLSRNDDLERATGGRPGDAGVD
                     GAITVWESSKRAEHGRASAFDGIPHALPALTRAQKVGRKAEALGLGGGAEALGLGGGA
                     DDLRGAVAAVQRDPTDVEAFGVLLAAVVAAARAGGVDPEDALRRRVDADEAVYRSRES
                     ESEG"
     gene            1849..3153
                     /locus_tag="PLZ72_03075"
     CDS             1849..3153
                     /locus_tag="PLZ72_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNA35313.1"
                     /translation="MPQPLQIAPASGRLGILTPGMGAVASTAYAGVLAARQGLAVPIG
                     SLTQMAHIRLGRREEGRNPLIRDFAPLAQLDDLVFGGWDPISPNALEAARTAGVLEER
                     DLAPLSGELEGINAMPAVFDQRWVKRLDGVRVKTGESKWDLAQQLIADIERFRTENEL
                     DRLVMVWCGSTEAYQEPSAVHESVEAFEAGLRADDENISPSQLYVYAALASGVPFANG
                     APNLSTDLKCMEELALRNGVPITGKDFKTGQTLMKTILAPGFKARMLGMRGWYSTNIL
                     GNRDGEVLDDPENFKTKEVSKLGVLDTILQPEVYPDLYGNIDHVVRINYYPPRGDNKE
                     GWDNIDIFGWMGYPMQIKVDFLCRDSILAAPIVLDLALFLDLAHRAGQSGVQEWLSFY
                     WKSPQPAGGVSPEHDIFIQQTKLKNTLREWMGEPAVTHSEAE"
     gene            3150..3923
                     /locus_tag="PLZ72_03080"
     CDS             3150..3923
                     /locus_tag="PLZ72_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNA35314.1"
                     /translation="MIVTGDPAVPVPVGTDRLRTDSARVTELVMTDLAAAVPSCPGWD
                     LRSLVEHLGGVHRWVLEAVTTGAAPARARATDPLSEPAPEAPDALARWFGDGAIALAH
                     ALDVAGADTPTWTPFPIEDPTVAVWIRRQTHETSLHRWDAELAVAVPRAIDAELASDG
                     IDEYLTVIVPRLVQRDGVVLPSGSVHLHCTDVPGEWTVEVLDGRYSVDRTHRKGDAAV
                     RGTAEDLLLALWGRPVPDGAPEVLGDVEVGAAWMGIGGS"
     gene            3991..5874
                     /locus_tag="PLZ72_03085"
     CDS             3991..5874
                     /locus_tag="PLZ72_03085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNA35315.1"
                     /translation="MATATSSSSAPPKLGYRPSLDGLRALAVTAVVLYHADISWMSGG
                     FLGVEVFFVVSGFLITALLIDERHHSGRISLRQFWTRRARRLLPALYLLLFVVSVASL
                     LVYRDAAGRMGGDVLAALLYVSNWWQIYLQESYFAQAGRPPLLQHLWSLAIEEQFYVV
                     FPALFAWGMARAGRRRMAWILGGLALASAAWMALRFEEFTDPSAVYYSTFTRASGLLL
                     GALLAVVWAPWRTRGRAAASAGAVLDGAGLAGLLLIGWFFWRVNAFDPFIYRGGMLLL
                     DVICVVVIAVLVHPACRLHKVLGLAPLRWIGLRSYSIYLWHWPIFMVTRPELDLPFTG
                     WPVFVLRIGLTMGAAEMSYRYVETPLRQGAIGSWWRGFRQSSGERRAVYARQGTIVAG
                     TSLVLIALIGMGLQRAASSPDREKIALEAAAVPDVDDGSGPASTEVPVPDTTSTTVSV
                     PAGQSATTTIVPVTTVPTVAAGVTQTATNALAVGDSVMLGATGPLKTAMPGLTVNAKV
                     GRQFDTVLQVLQWFISEGKAPGPVIIHAGTNGTFSDGDLDRLFEIAGDRPVLLVNAKV
                     ERPWQDLVNSRLAAAAERHPNAVLVDWHGLSEGHPEWFAPDGAHLRPAGARAYAELIR
                     SNL"
     gene            5965..7260
                     /gene="eno"
                     /locus_tag="PLZ72_03090"
     CDS             5965..7260
                     /gene="eno"
                     /locus_tag="PLZ72_03090"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNA35316.1"
                     /translation="MSIIEGIWAREILDSRGNPTVEVEVELDSGAMGRAAVPSGASTG
                     AFEAVELRDGDADRFGGKGVTDAVDNVNGEIADALLGLDAVDQRDLDRILIDLDGTDN
                     KGRLGANAILGVSMAAAKAVATELDLPLYRYVGGPNACVLPVPMLNVINGGEHADNNV
                     DLQEYMFMPVGAASFSEAVRWGAECYHTLKKVVGDRGLSTAIGDEGGFAPNLGSNEEA
                     VALLVEAIERAGRTPGDEIAIALDAASTEFFADGVYTLAGEGRSLSSAEFADYLADLC
                     DRYPIVSIEDGMAEEDWDGWAALTAKVGDRVQLVGDDLFVTNSERLARGIDLGVANSI
                     LVKVNQIGSLTETLEAVELATRHSYTSVMSHRSGETEDVTIADLAVATNCGQIKTGAP
                     ARSDRVAKYNQLLRIEEQLGEAAVYRGAAALAPLGKRPA"
     gene            7257..7640
                     /locus_tag="PLZ72_03095"
     CDS             7257..7640
                     /locus_tag="PLZ72_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNA35317.1"
                     /translation="MSAAGDPHRRPMALIGRRTAVVATVVAVTLVLVALPARSYLSQQ
                     SDVAEARAELAELERANDRLEVRQERLSDPDEIARIARRDYGLVDVGQESYSVLPTAA
                     AGLVMPRAWPFEQIEDAVRATSGGG"
     gene            7633..8112
                     /locus_tag="PLZ72_03100"
     CDS             7633..8112
                     /locus_tag="PLZ72_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HNA35318.1"
                     /translation="MAEPVADRAADLEAVERLIGRRPLAAFDVVVRRSNGAPVVIRNA
                     PLLADGRPMPTRYWLVDPELNRAIGRLEADGGVDRAEAEIDAEVLAEAHRRYAAERDA
                     AIPADHVGPRPSGGVGGTRTGVKCLHAHYAHLLATGDDPVGAWVRDRLPEPEDPPRG"
     gene            8105..9019
                     /locus_tag="PLZ72_03105"
     CDS             8105..9019
                     /locus_tag="PLZ72_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNA35319.1"
                     /translation="MAEPVAAIDCGTNSVRLLVTDGTRTLSRLMRITRLGQGVDATGR
                     LDPDAVERTLEVLGEYRAEMDRLGATRVRMAATSASRDAANRHEFFERATTILGAEPE
                     LLSGEEEAQLSFRGATADLDPTDGPFVVVDIGGGSTEFAVGGTELEGAVSVDMGCVRL
                     TEKYLHGDPPRPEELANALSIVGQHLADVQRLLPSVGAPSRFIGLAGTVTNVAAVEIG
                     LATYDPEVIHHFVLSRDAVEDVFRTLATEAWADRIHNPGLERERADVIVGGCCVLVQI
                     FRTFGFDECLVSESDILDGLAASLLDAG"
     gene            9079..9708
                     /locus_tag="PLZ72_03110"
     CDS             9079..9708
                     /locus_tag="PLZ72_03110"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNA35320.1"
                     /translation="MLRRKPLPDPTIAPVTLNGRRVVLRPLSVHDFDTWREVRRRCND
                     WLTRWEPRRIPGQPDTVEDRAAFSSRCSVRMREIQLGTGYGFGIFVDGDFAGEINVNS
                     IHRGAHQNAYVGYWIDERLAGQGYMPESVVAVLRFCFEQLLLHRVQISIIPRNAASRR
                     VVEKLGLRPEGVAERYLEINGVWEDHIRYAMTVEEWELRRDELTTVWLL"
     gene            complement(9737..10492)
                     /locus_tag="PLZ72_03115"
     CDS             complement(9737..10492)
                     /locus_tag="PLZ72_03115"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNA35321.1"
                     /translation="MPTVHVENREGVALLILDDPERRNALSVELCAAIVAAVEELEAD
                     ESVGALVVTGAGPAFCAGADLSQLGGSRRDGLLGIYEGFLRVARSPLPTIAAVNGAAV
                     GAGMNLALGCDVRLAGRSARFDTRFLDLGIHPGGGHTWMMQRAVGLQATMATVVFGEV
                     LDGVEAERVGLAWRCVGDDELVDAAVAMAARAASAPRELTRRVRASVLASTVIDDHDH
                     AVERELVDQLWSMDQPAFAERLAALQRRISSQG"
     gene            10539..11783
                     /locus_tag="PLZ72_03120"
     CDS             10539..11783
                     /locus_tag="PLZ72_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA35322.1"
                     /translation="MESPEVRTLVVAELYPWPPVDGYRQRLHHIVGGLSQAGPLDVFA
                     PLRDHGTEPVAPPWPGVARTLTVPMADPAGPRSWIADWSRSEVPRRLLSLDWSSSVDA
                     LERWRDRYDLIWYSHVDAWWPVHQLFDGVPAIVDFDNLENLSMRLRRRIPPRIDQAAG
                     PRDRAAALARWATSRAFDVIDERRWDSIQRRCADAVDHVVVCSDLDVERSGCANAVVV
                     PNGAEAPEDRRTDRRELAGVAPSLLFVGALDYEPNTEAMSWFLRDVWPIVRRGRPEAE
                     LRVVGRGAEALGSSAEADGVRMLGTVPDLRTELERADVSIVPIRVGAGTRLKVIEALA
                     HHLPLVTTTVGCEGIDVIDGTHALVADGAPGFAGAVLRLVADGGLRQRLADAGRVLFE
                     ERYAWSAIRDDVEALARRTVSP"
     gene            complement(11790..12836)
                     /locus_tag="PLZ72_03125"
     CDS             complement(11790..12836)
                     /locus_tag="PLZ72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA35323.1"
                     /translation="MLVTGLGTFWGGRVAQALEADENVEVIVGLDTTEPTVELERTEY
                     VRVDANYSILARIVRAAQIDTIIHTFLVVDSTQMSRRATHEINVIGTMNLFAAASAPA
                     STVRTVIVKSSTMVYGCSAADPIWFDEQSTRSTVARTGVERSLESVEGYVRDFAEDNP
                     HVNVALLRFSNVIGADIETPFTRALELPMVPSLFGFDPRFQFVHEDDVIRSILFTLDH
                     DLPGVYNVAGDGLLPWSEVAALCGKRTVPLPPFGAGLITAPLAQFGLLDLPDEHRLLL
                     RYGRGVDNRRLKDLGFRYEYTSAEAVLAFIEAVRLRRTVGDSAPVYRYERDVEQFFRH
                     SPAVVRDERSTVRD"
     gene            complement(12866..>13245)
                     /locus_tag="PLZ72_03130"
     CDS             complement(12866..>13245)
                     /locus_tag="PLZ72_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35324.1"
                     /translation="QLRRFGRGGFVEIAMRAGVPVIPIAVVGAEESMPTLARIPYLPS
                     LVGIPYAPLTANMLLMGPLGLVGYLPAKFKLRVLDPVHFDVPPDQERYSRSRVMDESE
                     AIRDRIQSALYDMLRRRRSVWFG"
     assembly_gap    13246..13345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13346..13590)
                     /locus_tag="PLZ72_03135"
     CDS             complement(<13346..13590)
                     /locus_tag="PLZ72_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35325.1"
                     /translation="MTSTEGRDTTDGTAVDADLAELASTIDPPARASTRALVPVPAPE
                     SDEDDEHVDVRLSDVDEWGRSERMRALARRIYDPMYRR"
     gene            complement(13631..14086)
                     /locus_tag="PLZ72_03140"
     CDS             complement(13631..14086)
                     /locus_tag="PLZ72_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNA35326.1"
                     /translation="MADPPIDEIDAELIRHLQLDGRRPYTQLAREVGLSEAAVRQRVQ
                     RLLEHGVMQIVAVTDPLQLGLHRQAMILIEVDGDVREVAAALEQFDEVDYLVLTAGSV
                     DILAEVFVPDDAALLRLLNDRIRKVPGVARTESVIYLQLTKQTYTWNVP"
     gene            complement(14100..14825)
                     /locus_tag="PLZ72_03145"
     CDS             complement(14100..14825)
                     /locus_tag="PLZ72_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35327.1"
                     /translation="MSDPITVGLILCDHLDPGPAAVAGDYPDLFPAAFAPHGLELRVY
                     EATLGELPEDLDECDVWMVSGSRRSAYEDEGWIAAVSDLIVRIEAARRPLAGICFGHQ
                     LTARALGGEVTRAEVGWGVGGRSFDVVAPAPWMDDGDRFTLLMSHRDQVTRLPDGAEV
                     VATAPYCPVGAYRIGDHVFCVQGHPEFVPDLSLALMEARREIIGDAVVDAGVASLGAG
                     APPLDQDRVAGWISEFYRRALGR"
     gene            complement(14822..16003)
                     /locus_tag="PLZ72_03150"
     CDS             complement(14822..16003)
                     /locus_tag="PLZ72_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA35328.1"
                     /translation="MAEIPDLSSTGTRWPVAIHWFLPTGGDSREVVPADPSASRAPDH
                     GYLAQVATACDHLGFDAVLTPCGTGCEDAWVATASLIPLTRRLRFLVAFRPGFLNPTQ
                     AAQMAATYQAMSGGRLMINIVIGAEPAELARFGDHLSKDERYRRSAEFLTVLRGALGS
                     TSFDFDGDFYRVEAATTRHSPVESPPIFFGGASDAAEAVAATHVDTYLLWGEPPDAAG
                     ERLERMRERAAATTRDTGRELEFGIRFHVITRDTAEEAWDEAARLLRGMDPDAVAAAR
                     ADFAATASVGQRRMADLHAEVDGVPEDPRDLEISPNLWAGVGLVRGGAGTVLVGSHSE
                     VADRIEEYHRLGFREFILSGYPHLEEAWRVGDGLLPELRRRQLIGPAPGTRPAEVFTF
                     R"
     gene            complement(16008..17405)
                     /locus_tag="PLZ72_03155"
     CDS             complement(16008..17405)
                     /locus_tag="PLZ72_03155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA35329.1"
                     /translation="MTVPDVERVRSRSLWLETCGDDLTPRPSLSGDTDADVVVVGGGL
                     TGLWTALYLREQAPDCRVVVLEAEVCGFGASGRNGGWCSALFPTSLDQLAVQAGRDAA
                     VAQHRAMIDTVDVVGREVERLGIDCHWARGGTVTVATNPAHVGRIRSYVDERHRWGFG
                     EADDRWLSPTEMAERVRPVGGAGGGYTPHCASVHPARLVRGLARAAERAGVTVHEGTR
                     VTGIATGAVTTAGGRVRADTVLVCTEGYTARLPGEKRRMLPLYSLMVATEPLSAEVWE
                     RIGLAERETFSDGRHLLIYGQRTADDRFAFGGRGAPYHFGSAITDAYDRDRRVAEELA
                     AVLRQLFPALTDAAIDHHWGGPLGVPRDWAASVTFDRSRGIGTAGGYVGDGVSTTNLA
                     GRTLADLVLGHDTERVRLPWVGHRSRDWEPEPLRWIGVNTGRVLAATLDRREARRRRP
                     SPLLEGLFDRVTGGH"
     gene            complement(17402..18769)
                     /locus_tag="PLZ72_03160"
     CDS             complement(17402..18769)
                     /locus_tag="PLZ72_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA35330.1"
                     /translation="MGTDRDDQLTRRAEEIAAVEMPRLLERTRGSEALFQRAVGSMPG
                     GVASSFQLGDPYPVYLSRGVGAEVWDVDGNAYFDFHNGFGSMAVGHAHPVVAEAVEHA
                     ARNGMHFAVTVEQTVALAEELCRRFRVEQVRFTNSGTESNMSAIRVARAATGRDVIAK
                     IEGSYHGHVDQLMYSVLPGADVMGGRDAPAATPKSKGMPEGLAEWIRVVPFNDAAALE
                     RLLAAEGDRIACLIMEPVMMNIGIVVPADGYLQAVRDLCTRYGVVLIFDEVKCGATIA
                     EGGAQERFGVRPDLATWAKAIGGGAPIGAFGGRADIMEEINRGAAHQGTFNGNPMSVT
                     AALATLTRVLTADAYEHFDALGARLSNGMDALIAEYDLPAHTVDLGCKGCISYRAEPL
                     TCYRDFLETNIHLYTASYPWMVNRGVFMTPGDEEQWTLSVQHSEVHVDRYVDAFATFA
                     AELTR"
     gene            complement(18784..20217)
                     /locus_tag="PLZ72_03165"
     CDS             complement(18784..20217)
                     /locus_tag="PLZ72_03165"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HNA35331.1"
                     /translation="MADIEVRNQNFIGGRWAPARSGATIDIVDPATGSVVFTSPDSGA
                     DDVDDACRAAADAFGGWGRTTPAERMAALLRFADAIEARADDLAAVEHRNAGKPIAAV
                     GVEIEASVDCYRFFAGACRTMETQGAGEYLEGYTSMLRREPLGVVASIAPWNYPFMMA
                     AWKLGPALAAGNCVVLKPSELTPMSALLMAEIAADVFPAGVLNVVAGGKQTGATLVDH
                     PLVEMISLTGSVGTGKAIARAASESLKRVHLELGGKAPVVVLDDADLEVVAESIKVGG
                     YWNAGQDCTAACRVITGAGIHDAAVDAISEAARSLVVGPTTDEATELGPVISAAQRDR
                     VQGFVDRAVAAGATAVTGGALGSGDGFYYEPSVIVGVDQHAEIVQREVFGPVVTVQSA
                     PDLAAAVAMANDVDYGLAASVFTRDVGQAMEASALMRFGTVWVNDHIPLVSEMPHGGF
                     KQSGYGKDMSQYAVEHYTELKHVMVKW"
     gene            20373..21569
                     /locus_tag="PLZ72_03170"
     CDS             20373..21569
                     /locus_tag="PLZ72_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35332.1"
                     /translation="MAEPTGTAERNGGGSAVVRLEQVTKHFGDFVAVEEADFDIGEGE
                     FFSMLGPSGCGKTTTLRMIAGFEQPTTGRILLQGQDVSRTAPHKRDVNTVFQNYALFP
                     HMNVFDNVAYGLRNKKVKEAEVATRVNEMLEVVRLPEFAQRKPNQLSGGQQQRIALAR
                     ALVNMPKALLLDEPLGALDLKLRQVMQLELKRIQRDVGITFIFVTHDQEEALTMSDRI
                     AVMTQGRVDQIGTPTEIYGNPATTFVAGFIGTANLLPVSVVSQTGDQVTVDSRLGTQF
                     RVPAGGRSYGTGADATIMIRPERLVPAGAPLHGGEAAGSIECTVSSLVFQGPVVRCQV
                     QTRTGADLVAHLGPEDDPASLSVGSPITMTWAYEGAYLVPEAIAADQAGVTSMDEDET
                     EAVSVG"
     gene            21654..22823
                     /locus_tag="PLZ72_03175"
     CDS             21654..22823
                     /locus_tag="PLZ72_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNA35333.1"
                     /translation="MANRVTRRQFMTRSGLAVGGAVIAPQLLAACGGDDESGGGSDAS
                     AKGVYFENWPEYIDTAKDGSVDGPGTTLANFTEQTGIPIKYTETYNDNNEYFAKIQPL
                     LSAGKPIGPNVIAPTYWMVARLIDLGWLEKLPLDKIPNSKNLVPNLVKPPSDPTGEYS
                     LPWQAGTTGIAYNVKATGGKELTSVEDLWDPALKGKISMLTEMRDTIGLIAMAEGIDI
                     STANTIEQYQPAFDTLKEQVDNGQIRQFTGNEYVGPLEDGSLAACFAWSGDVLGMENP
                     DIRYVVPDSGGTLWFDAMVIPKGASNVDDVAEWMNFVYDPVNAAQITAAVQFISPVQG
                     VQEQLRKMGGEDAALADNPLLFPDDATKARLHTFANLSDDQEAKFDEEFSKISGA"
     gene            22865..23731
                     /locus_tag="PLZ72_03180"
     CDS             22865..23731
                     /locus_tag="PLZ72_03180"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35334.1"
                     /translation="MADDKALRRGRWAPMGMLSPGIIWLLLFFLVPVAMLFKASLSTK
                     PSRFENPVFDWNFSNYADALSQYQPEFIRSFLYAGTATVLAIVIGYPLAYWIATQGGR
                     YKNLLIGLVVVPFFTSYLIRTIAWTSILRDDGPFVSMYQLLPWVGDDGRILATPWAVI
                     GGLTYNFISFMILPIYVSLEKIDFRLTEAAADLGSTAWGAFRKVVLPLSMPGVFAGSL
                     LVFIPAAGDYINNRYLGSAKTSMIGTVVQNQFLVQVDFPQAAALSMVLMVIITVLVLI
                     YSRFLGTEGLAV"
     gene            23728..24606
                     /locus_tag="PLZ72_03185"
     CDS             23728..24606
                     /locus_tag="PLZ72_03185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35335.1"
                     /translation="MTMSGGAAPPVMVETADLDRARNAGPMKVRRRWGRRLLTAFAAL
                     VFLWLFAPLINIVIFTFNKPAGRRNLSWNEFTLENWSQPFKDTDLTSAFMRSLLVSAV
                     AVSVALVLGSLMAIALSRYRFTGSKVTEIFLVLPLTTPEIVLGASLYQLFLGTNTTLG
                     FSTIVIAHIMFCVSFVALTVKARVRGFDWTLEDASLDLGCSPWRTFRKVTFPLILPGI
                     MAAALLSFALSLDDYIITDFTKGEYVTFPIQVNNQFRISFPPQVNVLATIILVVSVLL
                     LVVTSVREDRSSVVGR"
     gene            complement(24670..26181)
                     /locus_tag="PLZ72_03190"
     CDS             complement(24670..26181)
                     /locus_tag="PLZ72_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNA35336.1"
                     /translation="MCSIPDHDATHGDDPDDVVADPPRAEGGTAARPAGPGFTGFGPE
                     QVRSIARSHSALAAARLLAATDATALTDDECLGVFDDLEVLQRSIDAIGVMVLTEIDD
                     RDLCDTRYGTTSRVWFERRHGRSRVAIGRQASRGRRLRRDLPQIAAALARGEITGERA
                     LYVASKLNDRNADALAAAQQALLALSAAEPSFAQFCVLVTDLARFADADGAEDPDPVT
                     SSAQLHRVDDEVVLSATYVDVDGESFEQLVEAATNALWRRWRRDCDANPDLEMPTRTQ
                     IRAQALHELVRRGAAADPKATRPTVTDLSLVVDADRLDELDELDPILAGLLDGIGHHV
                     DPHRHRFDENGHVCCGHGGRLGVPVTSPDGRRLTISARQWEMLVCNASVSEVLLDAMG
                     MPVAVRDRLRHPTAAMRRGLVVRDGGCAFPGCDHPPEWCDAHHVVHWADDGRTVMVNL
                     VLLCRRHHGVVHRDGWSVSPNDDRAEGDGFFTITTPSGLTMETQHRHRSPVPA"
     gene            26268..26879
                     /locus_tag="PLZ72_03195"
     CDS             26268..26879
                     /locus_tag="PLZ72_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNA35337.1"
                     /translation="MRTIEVFADVTCPFTHVGLRRMVAARQAQGRDDVVLRVRSWPLE
                     VVNGAPLDAAFVAEEVDDIRAQVAPDLFVGFNSERFPSSAMTAMALAAAADRVDPITG
                     ERVSLALRTMLFEEGVDISDESVLRSVAAEHGVDWSDVDPARVLADHAEGIERHVTGS
                     PHFFTPGGDFFCPGLAISRGDDGHLHVEPKAEVFEAFLASCFA"
     gene            27011..27349
                     /locus_tag="PLZ72_03200"
     CDS             27011..27349
                     /locus_tag="PLZ72_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35338.1"
                     /translation="MSALARRRGLQVPVFRSPPGLDDVDRTLRRRADGAVVVAIRLTG
                     RPFAAVQGDVIAGIVAANGLTGRDAGRFRRAAWTALGDTPGGGSDRAVAGWGGPAAST
                     DATTPNVGVA"
     gene            27355..27612
                     /locus_tag="PLZ72_03205"
     CDS             27355..27612
                     /locus_tag="PLZ72_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35339.1"
                     /translation="MAKVVQVRNVPDRVHAVLTRRAAEAGLSLSDYVLRELELVASRD
                     ANSELLHQLALLPRSAVSGADAVRSMRDERDRGIARRVARP"
     gene            27596..27982
                     /locus_tag="PLZ72_03210"
     CDS             27596..27982
                     /locus_tag="PLZ72_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35340.1"
                     /translation="MSLVLDASAAVELVLGRPGLARLLLGEELHVPAHFDVEVVAALR
                     GVQIRGEASAEDVERATNAFRQLVVVRHWATLLVDRAWDLRDAITIQDGAYVALAEGL
                     GCALVTTDVRLERSASGLVPIVTPDS"
BASE COUNT         3944 a         9697 c        10195 g         4135 t
ORIGIN      
        1 ggccgacgcc cgcgccgccc tgcgcaaggg cgcggatctg gccgacctcg aggagtccgt
       61 gcaggggagt cgcctggcgc tgaccaccga gcgctgcgtc ccccgccagt acctccccga
      121 ggcgctgcgc accccggtcg cctcggcccg catcggggcc ctggtcggcc cggtgcccgg
      181 ggtcttcccc ggtgacgatg ccgtggtgtg gttccgggtc gagtcggcca aggtcgttcc
      241 gtactccgac gccggtgacg ccctggccga gctggtccga ctcatcgccc agcagggggt
      301 ggagtcctgg ttgaacctca aggccaacgc gctggtcgac atcgacccgc gctacggcag
      361 ccaggtcgag gtcggccaac agggcctcac cgtgctcgct ccggccaccc cggtcgggac
      421 gccctgacgg tccaccggtg ctgcacctgg tcgggctcgg accggcgggg cctgacctgg
      481 tcaccgtcgg gacccgggac ctgctcgacg gcggtcggcc cgtcttcctg aggacccggc
      541 gccacccggc ggcgtcggtg gtgcccgacg gcaccgtcga cttcgacgac gtgtacgagg
      601 cggcagatcg catcgaggac gtctaccggg agatcgtcga gcggctcgtc gacgccgagg
      661 cgcggcacag ctcggcggtc tacgcggtgc cgggttcgcc ggtggtggcc gagcacaccg
      721 tcgagctgct cgtcgccgat ccccgcgtcg aggtcgtggt gcatcccgcc ctgtcgttcc
      781 tggacctcac ctgggcgcgg ctcggggtgg accccgtcga gcggggggtc cgtctggtcg
      841 acggtcaccg cttcgacagc gaggccgcgg gggagcgcgg cccgctgctg gtcggccagt
      901 gcgactctct cttcgtcctg tccgacatca agctggccgt cgaggacccg ccccgggaac
      961 ccgtcgtggt cctccagagg ctcgggctgc ccgacgagct cgtcgccgag gtcgcctggg
     1021 aggacctcga ccgcgtcgtg tcgccggacc acctgacctc gctgtggatc ccccggctgg
     1081 ccgcaccggt cgcgtccgac gtggcccgct tcgaggcact gctgcgccgg ctgagggtga
     1141 gcgacccctg gaaggcggag cagacccacg actcgctcaa gcggttcctg ctcgaggagt
     1201 cctacgaggt gctcgaggcg atcgacgcct acgaccccga gtcgggagcg ggggccgacg
     1261 agctgaccag tgagctgggc gatctgctgt accaggtggt gttccacgcc gcgttgggag
     1321 ccgaggccgg atggttcgac ctgtccgacg tggcccgggc gatccacgac aagctcctca
     1381 gccgcaacga cgacctggag cgggcgaccg gcggccgtcc gggcgacgcc ggggtcgacg
     1441 gggcgatcac ggtgtgggag tcctcgaagc gcgcggagca cggccgggcc tcggccttcg
     1501 acgggatccc ccacgccctc ccggcgttga cccgggccca gaaggtcggg cgcaaggccg
     1561 aggcactcgg cctgggtggt ggggccgagg cactcggtct gggtggtggg gccgacgatc
     1621 tgcgcggggc ggtcgccgcg gtgcagcggg atcccaccga cgtcgaggcg ttcggggtcc
     1681 tgctggccgc agtggtcgcc gcggcgcggg cgggcggcgt cgaccccgag gacgccctgc
     1741 ggcgacgcgt cgacgcggac gaggccgtct accgctcccg cgagagcgag agcgagggct
     1801 gagcgccggc ccggtgggtc gctgtggggg tgtcgtagag tgcggtccat gcctcaaccg
     1861 ctgcagatcg cccccgcgtc cggtcgtctc gggatcctca cccccggcat gggcgccgtc
     1921 gcctccaccg cctacgccgg ggtcctggcc gcccgtcagg gcctggccgt ccccatcggt
     1981 tcgctcaccc agatggccca catccgcctg ggtcgccgcg aggagggtcg caacccgctg
     2041 atccgcgact tcgcccccct ggcgcagctc gacgacctgg tcttcggcgg gtgggacccg
     2101 atctcgccca acgcgctcga ggcggcgcgg accgccggtg tcctcgagga gcgcgacctg
     2161 gcccccctgt cgggcgagct cgaggggatc aacgccatgc cggcggtgtt cgaccaacgc
     2221 tgggtcaagc gcctcgacgg cgtgcgggtc aagaccggtg agtccaagtg ggacctggcc
     2281 cagcagctga tcgccgacat cgagcggttc cgcaccgaga acgagctcga ccgcctggtc
     2341 atggtctggt gcgggtccac cgaggcctac caggagccgt ccgcggtcca cgagtcggtc
     2401 gaggcgttcg aggctggtct gcgcgccgac gacgagaaca tctcgcccag ccagctctac
     2461 gtctacgccg cgctggcgtc aggggtgccg ttcgcgaacg gggcgccgaa cctgtcgacc
     2521 gacctgaagt gcatggagga gctggcgctg cgcaacggcg tgccgatcac cggaaaggac
     2581 ttcaagaccg gccagaccct gatgaagacg atcctggcgc cggggttcaa ggcccggatg
     2641 ctcgggatgc ggggctggta ctcgaccaac atcctcggca accgcgacgg cgaggtgctc
     2701 gacgacccgg agaacttcaa gaccaaggag gtctccaagc tcggcgtgct cgacacgatc
     2761 ctgcagcccg aggtgtaccc cgacctctac ggcaacatcg accacgtcgt gcgcatcaac
     2821 tactacccgc cccgaggcga caacaaggag gggtgggaca acatcgacat cttcgggtgg
     2881 atgggctacc cgatgcagat caaggtcgac ttcctgtgcc gcgactcgat cctggccgcg
     2941 ccgatcgtgt tggacctcgc gctgttcctc gacctggcgc accgcgccgg gcagtccggg
     3001 gtgcaggagt ggctcagctt ctactggaag tcgccccagc cggccggcgg ggtctctccc
     3061 gagcacgaca tcttcatcca gcagaccaag ctcaagaaca cgctgcggga gtggatggga
     3121 gaacccgcgg tcacgcactc cgaggcggaa tgatcgtcac gggtgacccg gcggtcccgg
     3181 ttccggtcgg caccgatcgt ctccggaccg actcggcccg ggtcaccgag ctggtcatga
     3241 ccgacctcgc cgccgcggtc ccgtcgtgtc cgggctggga cctgcggagc ctggtcgagc
     3301 acctgggagg agtccaccga tgggtcctcg aggcggtgac gaccggggcc gctccggcgc
     3361 gggcgcgggc caccgatccc ctttccgagc cggcgccgga ggcacctgac gccctggccc
     3421 ggtggttcgg tgacggggcg atcgccctgg cccacgcgct cgacgttgcc ggtgcggaca
     3481 ccccgacctg gacgccgttc ccgatcgagg atccgaccgt ggcggtgtgg atccgacgcc
     3541 agacccacga gacgtcgctg caccgctggg acgccgagct cgccgtcgcg gtccctcgcg
     3601 ccatcgacgc cgagctcgcc tcggacggga tcgacgagta cctgacggtg atcgtgcccc
     3661 ggctggtgca gcgcgacggc gtggtgctgc cctccgggag cgtccacctc cactgcaccg
     3721 acgtccccgg cgaatggacc gtcgaggtgc tcgacggccg gtattcggtc gaccggaccc
     3781 accgcaaggg cgacgccgcc gttcggggga ccgccgagga ccttctcctg gccctgtggg
     3841 gtcggccggt tcccgacggc gcgcccgagg tcctcggcga cgtcgaggtc ggtgccgcct
     3901 ggatgggcat cggcggaagc tgagggcgac ggacgccccg ctcggggccg tccacgggcg
     3961 tgggcgctgc tcggtccaga atggggcgca atggccacgg ccacctcttc ttcctccgcg
     4021 cccccgaagc tgggctaccg gccgtcgctc gacgggctgc gcgccctcgc ggtcacagcc
     4081 gtggtgctgt accacgcgga catcagctgg atgtccggcg ggttcctcgg cgtcgaggtg
     4141 ttcttcgtcg tcagcgggtt cctgatcacc gcgctgctca tcgacgaacg ccaccactcc
     4201 ggccgcatct ccctgcgcca gttctggacc cgccgggccc gtcggctgct gccggcgttg
     4261 tacctgctgc tgttcgtcgt gtcggtggcg tcgctgctgg tctaccgcga cgccgcgggc
     4321 cggatgggtg gcgacgtcct cgccgcgctg ctctacgtgt cgaactggtg gcagatctac
     4381 ctccaggagt cgtacttcgc gcaggccggt cgcccgccgc tgctgcagca cctgtggtca
     4441 ctcgccatcg aggagcagtt ctacgtcgtg ttccccgcgt tgttcgcgtg ggggatggcc
     4501 cgggccggtc ggcggcggat ggcgtggatc ctcggcggtc tcgcgttggc gtccgcggcg
     4561 tggatggcgt tgcgcttcga ggagttcacc gacccgtccg ccgtgtacta ctcgacgttc
     4621 acccgggcct cgggcctgct gctgggggcc ctgctcgcgg tggtgtgggc gccgtggcgc
     4681 acccggggcc gcgccgcggc gagcgcgggt gcggtcctcg acggggccgg cctggccgga
     4741 ctgctgctga tcggctggtt cttctggagg gtcaacgcct tcgacccgtt catctaccgc
     4801 ggtggcatgc tgctcctcga cgtgatctgc gtcgtggtga tcgcggtgct ggtgcacccg
     4861 gcgtgccgac tgcacaaggt gctcggcctg gctccgctgc ggtggatcgg gttgcgttcg
     4921 tactcgatct acctgtggca ctggccgatc ttcatggtga cccgacccga gctcgacctg
     4981 ccgttcaccg gttggccggt gttcgtgctg cgcatcggtc tgacgatggg tgccgcggag
     5041 atgtcgtacc ggtacgtcga gacgccgctg cggcaggggg cgatcgggtc ctggtggcgc
     5101 ggcttccgcc aatcgagcgg cgaacgccgg gcggtctacg cccgtcaggg gacgatcgtc
     5161 gcgggcacca gcctggtgct gatcgccctg atcggaatgg gactccaacg cgccgccagc
     5221 agtcccgacc gcgagaagat cgccctggag gccgccgcgg tgcccgacgt ggacgacggc
     5281 tccggcccgg ccagcaccga ggtgccggtc cccgacacca catcgacgac ggtgagcgtc
     5341 cccgccggtc agtccgcgac gaccacgatc gtgccggtga cgaccgtgcc gacggtcgcg
     5401 gcgggggtca cccagaccgc gaccaacgcc ctcgccgtcg gcgactcggt catgctgggg
     5461 gcgaccggtc cgctcaagac cgccatgccg ggtctgacgg tcaacgccaa ggtgggtcgg
     5521 cagttcgaca ccgtcctcca ggtgctccag tggttcatct ccgaaggcaa ggctcccggc
     5581 ccggtcatca tccacgccgg gaccaacggc acgttctccg acggcgacct ggaccgcctg
     5641 ttcgagatcg ccggtgatcg accggtcctg ctggtcaacg ccaaggtcga acgcccgtgg
     5701 caggacctgg tcaacagccg gctcgccgcg gccgccgagc gccaccccaa cgcggtgctg
     5761 gtcgactggc acggtctgtc ggaaggccat cccgagtggt tcgcccccga cggcgcccac
     5821 ctgcgccccg ccggtgcccg ggcctacgcc gagctgatac gcagcaacct gtagtcgcgc
     5881 cacccggtgg cctacggtgt ccgaggcgcg ggcaccgggc gatcgccggc cggcggtccg
     5941 ccacgatcgg atgaggagac acgcgtgagc atcatcgagg gcatctgggc gcgggagatc
     6001 ctggactccc ggggcaaccc gaccgtcgag gtcgaagtcg agctcgactc cggcgccatg
     6061 ggtcgggccg cggtgccctc gggagcgtcc accggagcct tcgaggcggt cgagctgcgc
     6121 gacggagatg ccgaccggtt cggcggcaag ggcgtcaccg acgcggtcga caacgtcaac
     6181 ggcgagatcg ccgatgcgct gctcggcctc gacgcggtcg accagcgtga cctggatcgg
     6241 atcctgatcg acctcgacgg caccgacaac aagggccgtc tgggcgccaa cgccatcctc
     6301 ggcgtgtcga tggcggcggc caaggcggtg gcgaccgagc tcgacctgcc gctgtaccgc
     6361 tacgtcggcg gccccaacgc gtgcgtgctg ccggtcccca tgctcaacgt gatcaacggc
     6421 ggcgagcacg ccgacaacaa cgtcgacctg caggagtaca tgttcatgcc ggtcggcgcg
     6481 gcctcgttct ccgaggccgt ccgctggggt gcggagtgct accacaccct caagaaggtc
     6541 gtcggcgacc ggggcctgtc gaccgcgatc ggcgacgagg gcggcttcgc cccgaacctg
     6601 ggcagcaacg aagaggcggt ggccctgctg gtcgaggcca tcgagcgggc cgggcgcacc
     6661 cccggcgacg agatcgccat cgccttggac gcggcgtcga ccgagttctt cgcggatggc
     6721 gtgtacaccc tcgccggcga gggtcggtcc ctcagctcgg ccgagttcgc cgactacctg
     6781 gccgacctct gcgaccggta cccgatcgtg tcgatcgagg acggcatggc cgaggaggac
     6841 tgggacggct gggcggcgtt gaccgccaag gtgggggacc gggtccagct cgtcggtgac
     6901 gacctgttcg tgaccaactc cgaacgcctg gcccgcggca tcgacctcgg cgtggccaac
     6961 tcgatcctgg tcaaggtcaa ccagatcggc tctctcaccg agaccctcga ggcggtcgag
     7021 ctcgccaccc ggcactccta cacgtcggtc atgtcgcacc gctcgggcga gaccgaggac
     7081 gtgacgatcg cggacctcgc cgtggccacc aactgcggcc agatcaagac cggtgccccg
     7141 gctcgctcgg accgcgtggc caagtacaac cagctgctgc gcatcgagga gcagctgggc
     7201 gaagccgccg tgtaccgggg cgccgccgcg ctcgccccgc tggggaagcg cccggcgtga
     7261 gcgcggcggg cgacccgcac cgccgtccga tggcgctcat cggacggcgc accgcggtgg
     7321 tggcgaccgt ggtcgccgtc actctggtgc tggtcgcgct gccggcgcgc agctacctct
     7381 cccagcagtc cgacgtggcg gaggcgcggg cagagctggc ggagctcgaa cgggccaacg
     7441 accgcctcga ggtccgccag gagcgactgt cggaccccga cgagatcgct cggatcgccc
     7501 gccgcgacta cggactggtc gacgtcggcc aggagtccta ctcggtgctc ccgaccgcgg
     7561 cggcgggtct ggtgatgccc cgcgcctggc ccttcgagca gatcgaggac gcggtgcgcg
     7621 ccacctccgg cggtggctga accggtcgcg gaccgtgccg cggatctgga ggcggtcgag
     7681 cggctcatcg ggcgacgccc actggcggcc ttcgacgtcg tggtccgccg ctccaacggt
     7741 gccccggtcg tcatccgcaa cgccccgctc ctggccgacg gccggccgat gccgacgcgc
     7801 tactggctgg tggacccgga gctgaaccgg gcgatcggac gcctcgaggc cgacggcgga
     7861 gtcgaccggg ccgaagccga gatcgacgcg gaggtgctcg ccgaggcgca ccggcgctac
     7921 gcggccgagc gggacgccgc gatccccgcc gaccacgtcg gcccccggcc ctcgggtggg
     7981 gtgggcggca cccgcaccgg cgtcaagtgc ctgcacgccc actacgcgca cctcctggcg
     8041 accggcgacg accccgtcgg tgcctgggtg cgcgaccgcc ttcccgaacc ggaggatcct
     8101 ccccgtggct gaacccgtcg ccgccatcga ctgcggcacc aactcggtcc gactcctcgt
     8161 caccgacggg acccggaccc tgagccggct gatgcgcatc acccgcctgg gccagggcgt
     8221 cgacgcgacc ggtcggctcg acccagatgc ggtggagcgc acgctcgagg tcctggggga
     8281 gtaccgagcc gagatggatc ggctgggagc gacccgggtg cgaatggcgg ccacctcggc
     8341 ctcgcgcgac gccgcgaacc gccacgagtt cttcgaacgc gccaccacga tcctgggggc
     8401 ggaacccgag ctgctgtccg gagaggagga ggcccagctc agcttccggg gcgccaccgc
     8461 ggacctggac ccgaccgacg gcccgttcgt cgtcgtcgac atcggtggcg gctcgaccga
     8521 gttcgccgtc ggtgggaccg agctcgaggg ggccgtgtcg gtcgacatgg gatgtgtccg
     8581 cctcaccgag aagtacctgc acggcgaccc gccccgcccg gaggagctcg ccaacgcgct
     8641 gtcgatcgtc ggtcagcacc tggctgacgt ccagcggctg ctgccgtcgg tcggagcgcc
     8701 gtcacggttc atcggcctgg ccgggacggt gaccaacgtg gccgcggtcg agatcggtct
     8761 cgccacctac gaccccgagg tgatccacca cttcgtcctg agccgcgacg cggtcgagga
     8821 cgtgttccgg accctggcga ccgaggcgtg ggccgaccgg atccacaacc cgggcctcga
     8881 gcgcgaacgg gccgacgtga tcgtgggcgg gtgctgcgtg ctggtgcaga tcttccgcac
     8941 gttcggcttc gacgagtgcc tggtgtcgga gtccgacatc ctcgacgggc tcgccgccag
     9001 cctgctcgac gccggttgag cgtgcccgga cccgccccgg acggagggtc cggcggatcc
     9061 gctcggtatg ctccgccggt gctgcgccgc aagcccctgc ccgatcccac gatcgcgccg
     9121 gtgaccctga acggtcgtcg ggtggtgctg cgccccctca gcgtccacga cttcgacacc
     9181 tggcgcgagg tgcgtcggcg ctgcaacgac tggctgactc gctgggagcc ccgtcggatc
     9241 cccggtcagc ccgacaccgt cgaggaccgc gccgcgttct cgtcgcggtg ctcggtgcgg
     9301 atgcgggaga tccagctcgg aaccggctac gggttcggca tcttcgtcga cggcgacttc
     9361 gccggcgaga tcaacgtgaa ctcgatccac cgcggtgcgc accagaacgc gtacgtcggg
     9421 tactggatcg acgagcggtt ggccggacag ggctacatgc ccgagtcggt ggtggcggtg
     9481 ctgcggttct gcttcgagca gctgctgctg caccgggtgc agatctcgat catcccgcgc
     9541 aacgcggcca gccgccgggt ggtcgagaag ctcgggttga ggcccgaggg ggtcgccgag
     9601 cgctacctgg agatcaacgg cgtgtgggag gaccacatcc gctacgccat gaccgtcgag
     9661 gagtgggagc tgcgccgcga cgagctcacc accgtgtggc tcctctgatc gacctgtgac
     9721 ggggctggac ccccgttcag ccctggctcg agatgcgtcg ctgcagggcg gcgagccgct
     9781 cggcgaacgc cggttggtcc atcgaccaca gctggtcgac cagctcccgt tcgacggcgt
     9841 ggtcgtggtc gtcgatgacc gtcgaggcga gcacgctggc ccgcacccgc cgggtgagct
     9901 cccggggtgc cgaggcggcc cgggccgcca tggcgaccgc tgcgtcgacg agctcgtcgt
     9961 cgcccacgca gcgccacgcc agaccgaccc gttcggcctc gacgccgtcg aggacctcac
    10021 cgaacaccac cgtcgccatc gtggcctgca gcccgacggc ccgctgcatc atccaggtgt
    10081 ggcccccgcc ggggtggatg ccgaggtcga ggaaccgggt gtcgaagcgc gccgagcggc
    10141 cggccaggcg gacgtcgcag ccgagggcca ggttcatccc ggcgcccacc gccgctccgt
    10201 tgaccgcggc gatggtcggc agcggtgagc gggcgacccg caggaagccc tcgtagatgc
    10261 cgagcaggcc gtcgcgccgc gaccctccga gctggctgag gtccgcgccg gcgcagaacg
    10321 cgggtcctgc cccggtcacc acgagagcgc cgaccgactc gtcggcctcg agctcctcga
    10381 ccgcggcgac gatcgcggcg cacaactcga ccgacagggc gttgcgacgc tccgggtcgt
    10441 ccaggatgag cagagcgacg ccctcgcggt tctcgacgtg gacggtgggc atgggtcctc
    10501 ctcggagcgg ggtgcgatcc cggtagggtc gtcacccgat ggagagcccg gaagtgcgca
    10561 cgctcgtggt ggcggaactg tatccatggc ctccggtcga cggttaccgc cagcgcctgc
    10621 accacatcgt gggcgggctg agccaggccg gtcctctcga cgtgttcgca ccgctgcgcg
    10681 accacggcac cgaaccggtg gcgccgccgt ggccgggagt ggcgcggacc ctcaccgtgc
    10741 cgatggccga tcccgccgga ccccggtcct ggatcgcgga ctggagccgc tcggaggtgc
    10801 cccggaggct cctgtcgctc gactggtcgt cgtcggtcga cgcgctcgag cgctggcggg
    10861 accgatacga cctgatctgg tactcccacg tcgatgcctg gtggccggtg catcagctgt
    10921 tcgacggggt gcccgccatc gtcgacttcg acaacctcga gaacctgtcg atgcggctgc
    10981 ggcgccggat cccgccgcgg atcgaccagg ctgccggacc ccgtgaccgg gccgcggccc
    11041 tggcccggtg ggcgacgtcg cgtgccttcg acgtcatcga cgagcgtcgc tgggactcga
    11101 tccagaggcg ctgtgcggac gcggtcgacc acgtcgtggt gtgcagcgat ctggacgtcg
    11161 agcgcagcgg gtgcgccaac gcggtggtgg tgcccaacgg agccgaggcc cccgaggacc
    11221 gccggaccga ccgtcgggag ctggcaggtg tcgcgccgtc gctgctgttc gtcggagcgc
    11281 tcgactacga gcccaacacc gaggcgatga gctggttcct ccgcgacgtc tggccgatcg
    11341 tccggcgggg tcgtcccgag gccgagctgc gggtggtcgg tcggggggcc gaggcgctcg
    11401 gttcgtcggc ggaggcagac ggggtgcgca tgctggggac cgtcccggac ctgcggaccg
    11461 agctcgagcg ggccgacgtg tcgatcgtgc cgatccgggt cggggccggg acgcggctca
    11521 aggtgatcga ggctctcgcc catcaccttc cgctggtcac caccaccgtc ggctgcgagg
    11581 gcatcgacgt gatcgacggc acccacgccc tggtcgccga cggcgcgccg ggcttcgccg
    11641 gggcggtgct gcggctggtg gccgacggcg ggctgcgcca gcgtctggcc gacgccggcc
    11701 gggtcctgtt cgaggagcgt tacgcctggt cggcgatccg cgacgacgtc gaggcgctgg
    11761 cccgacggac cgtcagtccc tgactgcagt cagtccctga ccgtggaccg ctcgtcgcgc
    11821 acgaccgccg gcgagtggcg gaagaactgc tcgacgtcgc gctcgtagcg gtatacgggg
    11881 gccgagtcgc cgacggtgcg tcgcagccgc acggcctcga tgaaggccag gaccgcctcg
    11941 gccgaggtgt actcgtagcg gaagcccagg tccttcagcc gacggttgtc gaccccgcgt
    12001 ccgtagcgca gcagcagccg gtgctcgtcg ggcaggtcga gcagcccgaa ctgggccagg
    12061 ggggcggtga tgaggccggc tccgaacggg ggcagcggga cggtgcgctt gccgcacagc
    12121 gctgcgacct cgctccaggg caggaggccg tcaccggcga cgttgtagac gccgggcagg
    12181 tcgtggtcga gggtgaacag gatcgaccgg atcacgtcgt cctcgtgcac gaactggaac
    12241 cgggggtcga agccgaacag cgacgggacc atcgggagct cgagcgcccg ggtgaacggc
    12301 gtctcgatgt cggcgccgat cacgttggag aaccgcagca gggccacgtt cacgtgcggg
    12361 ttgtcctcgg cgaagtcgcg cacgtagccc tcgacggact cgagcgagcg ctccaccccg
    12421 gtgcgggcca ccgtcgaacg ggtcgactgc tcgtcgaacc agatcgggtc cgcggcggag
    12481 cacccgtaga ccatggtcga ggacttcacg atcacggtgc gaaccgtcga cgccggcgcc
    12541 gaggccgccg cgaacaggtt catggtgccg atgacgttga tctcgtgggt ggcccgacgg
    12601 gacatctggg tcgagtcgac gaccaggaag gtgtggatga tcgtgtcgat ctgagcggcg
    12661 cgcacgatgc gggccaggat cgagtagttg gcgtcgacgc gcacgtactc ggtgcgctcc
    12721 agctcgacgg tcggctcggt cgtgtcgaga ccgacgatga cctcgacgtt ctcgtcggcc
    12781 tcgagcgcct gcgcgacccg accgccccag aaggtgccca ggccggtgac cagcacgcgg
    12841 ctgggacggt tcctgtcggt aggtctcatc cgaaccagac cgagcgtcgg cggcggagca
    12901 tgtcgtagag ggcggactgg atccggtccc ggatcgcctc ggactcgtcc atgacccgcg
    12961 agcgcgagta gcgctcctgg tcgggcggca cgtcgaagtg gaccgggtcg aggacccgca
    13021 gcttgaactt ggcgggcagg tagccgacca ggccgagcgg tcccatgagc agcatgttgg
    13081 cggtgagcgg tgcgtagggg atccccacca gcgacggcag gtacgggatc cgggccaggg
    13141 tcggcatgga ctcctcggcc ccgacgacgg cgatcgggat gacggggacc ccggcccgca
    13201 tggcgatctc gacgaacccg ccccggccga agcggcgcag ctggtnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnncggcg gtacatgggg tcgtagatgc gccgggccag
    13381 ggcgcgcatg cgttcggacc gtccccactc gtcgacgtcg gagagccgga cgtcgacgtg
    13441 ctcgtcgtcc tcgtcggatt cgggcgccgg gaccggcacc agggcccggg tcgaggcccg
    13501 ggccggcggg tcgatcgtcg acgccaactc ggcgaggtcg gcgtcgacgg ccgtcccgtc
    13561 ggtcgtgtcg cgcccctccg tcgatgtcat ggcggcgatg ttaggtcccc gtcgcgtcgg
    13621 cgacgagggg tcaggggacg ttccaggtgt aggtctgctt ggtcagctgc aggtagatca
    13681 ccgactcggt gcgggcgacg ccgggcacct tgcggatccg gtcgttgagg aggcggagca
    13741 gggcggcgtc gtcggggacg aacacctcgg cgaggatgtc gaccgaaccg gcggtgagca
    13801 ccaggtagtc gacctcgtcg aactgctcga gtgcggcggc cacctcgcgc acgtcgccgt
    13861 cgacctcgat gaggatcatc gcctgtcggt gcaggccgag ctgcaacggg tcggtgaccg
    13921 ccacgatctg catgacgccg tgctcgagca gacgttggac ccgttgacgc accgcagcct
    13981 ccgacagccc gacctcgcgg gcgagctggg tgtacgggcg ccggccgtcg agctggaggt
    14041 ggcggatcag ctcggcgtcg atctcgtcga tcggagggtc ggccatcgga tcgatcctct
    14101 cagcggccga gggcacggcg gtagaactcg gagatccagc ccgccacccg gtcctggtcc
    14161 aacgggggcg cccccgcgcc gagcgaggcg acgccggcgt cgaccactgc gtcgccgatg
    14221 atctcccgtc gcgcctccat cagtgcgagc gacaggtcgg ggacgaactc ggggtggccc
    14281 tgcacgcaga acacgtggtc accgatccgg taggccccga ccgggcagta cggcgcggtg
    14341 gcgacgacct cggcgccgtc ggggaggcgg gtgacctggt cgcggtggct catcaggagg
    14401 gtgaagcggt ctccgtcgtc catccacggc gcgggtgcga cgacgtcgaa gctccggccg
    14461 ccgacgcccc agccgacctc ggcccgtgtc acctcgccgc cgagcgcccg ggcggtgagc
    14521 tggtgcccga agcagatgcc ggccagcggg cgtcgtgccg cctcgatgcg cacgatcagg
    14581 tccgacacgg ccgcgatcca gccctcgtcc tcgtacgcgg accggcgtga ccccgacacc
    14641 atccagacgt cgcactcgtc gaggtcctcg ggcagctcgc cgagcgtggc ctcgtagacc
    14701 cgcagctcga gtccgtgcgg ggcgaaggcg gcggggaaca ggtcggggta gtcgccggcc
    14761 acggcggccg gaccggggtc gaggtggtcg cacaggatca ggcccaccgt gatcggatcg
    14821 ctcatcggaa cgtgaacacc tcggcggggc gcgtgcccgg ggccgggccg atcagctggc
    14881 ggcgacgcag ctcgggcagg agtccgtcgc cgacgcgcca cgcctcctcc aggtgcgggt
    14941 agcccgacag gatgaactcc cggaacccga gccggtggta ctcctcgatc cggtccgcga
    15001 cctcggagtg gctgccgacg agaacggttc cggccccacc gcggaccagg ccgaccccgg
    15061 cccagaggtt gggtgagatc tccaggtcgc gggggtcctc gggcacgccg tctacctcgg
    15121 cgtgcaggtc ggccatgcgc cgttggccga ccgaggcggt ggcggcgaag tcggcccggg
    15181 cggcagccac ggcgtcgggg tccatgcccc gcagcagccg ggccgcttca tcccatgcct
    15241 cctcagcggt gtcgcgggtg atgacgtgga agcggatgcc gaactcgagc tcccggccgg
    15301 tgtcgcgggt cgtggccgcg gcccgctccc gcatccgctc cagccgctcg ccggccgcgt
    15361 cgggcggttc tccccacagc aggtacgtgt cgacgtgggt cgcggcgacc gcctcggccg
    15421 cgtcggacgc gccaccgaag aagatcggcg gcgactcgac gggggagtgg cgggtggtgg
    15481 cggcctcgac ccggtagaag tcgccgtcga agtcgaacga ggtcgacccg agggcaccgc
    15541 gcagcacggt gaggaactca gccgaacggc ggtagcgctc gtccttggag aggtggtcgc
    15601 cgaagcgggc cagctcggcg ggctcggcgc cgatcacgat gttgatcatc agccgtccac
    15661 ccgacatggc ctggtaggtg gcggccatct gcgcggcctg cgtcgggttg aggaacccgg
    15721 gtcggaacgc gaccaggaac cggagacggc gggtgagggg gatcagcgac gcggtcgcga
    15781 cccacgcgtc ctcgcagccg gttccgcacg gggtgagcac ggcgtcgaag ccgaggtggt
    15841 cgcacgcggt cgcgacctgg gccaggtagc cgtggtccgg cgcccgggac gccgaggggt
    15901 cggccgggac gacctcgcgg ctgtctccgc cggtcggcag gaaccagtgg atcgccaccg
    15961 gccagcgggt tccggtcgac gagaggtcag ggatttcggc catcgcgtca gtggcctccg
    16021 gtcacgcggt cgaacagtcc ttcgagcagc ggggacggcc gacggcgtcg ggcctcacgc
    16081 cggtcgaggg tggcggcgag cacccgtccg gtgttgacgc cgatccaccg cagcggttcg
    16141 ggctcccagt cccgggagcg gtgcccgacc cagggcaggc ggacccgctc ggtgtcgtgg
    16201 ccgagcacca ggtcggccag ggtgcggccg gccaggttgg tggtggacac gccgtcgccg
    16261 acgtagccgc cggcggtgcc gatcccgcgg gagcggtcga aggtcaccga cgcggcccag
    16321 tcgcggggaa cgccgagcgg tccaccccag tggtggtcga tcgccgcgtc ggtgagggcg
    16381 gggaagagct ggcgcaggac cgcggcgagc tcctcggcca cgcggcggtc ccggtcgtag
    16441 gcgtcggtga tggcggagcc gaagtggtac ggggcgcccc ggccgccgaa cgcgaagcgg
    16501 tcgtccgcgg tgcgctggcc gtagatcagc aggtgccgac cgtcggagaa cgtctcgcgt
    16561 tcggcgagcc cgatgcgctc ccacacctcg gcggacagag gctcggtggc gaccatcagc
    16621 gagtagagcg gcagcatccg ccgcttctca cccggcagcc gggcggtgta gccctcggtg
    16681 cagaccagga cggtgtcggc cctgacccga ccgccggcgg tggtgaccgc tccggttgcg
    16741 atcccggtca cccgggtgcc ttcgtggacc gtcacgccgg cccgctccgc ggcgcgggcc
    16801 aggccacgga cgaggcgggc cgggtgcacc gacgcgcagt gcggggtgta gccgccgccg
    16861 gcgccgccca ccgggcggac ccgttcggcc atctcggtcg ggctcaacca tcggtcgtcg
    16921 gcctcgccga acccccaccg gtggcgttcg tcgacgtagc tgcggatccg cccgacgtgg
    16981 gccgggttgg tggcgacggt gacggtgccg ccgcgggccc agtggcagtc gatgcccaac
    17041 cgctcgacct cgcgtccgac cacgtcgacg gtgtcgatca tggcgcggtg ctgggcgacc
    17101 gccgcgtccc ggccggcctg caccgcgagc tggtcgagcg aggtggggaa cagcgccgag
    17161 caccacccgc cgttgcgtcc cgacgcgccg aagccgcaca cctcggcctc gagcacgacc
    17221 acccggcagt ccggggcctg ctcccgcagg tacagggcgg tccacagccc ggtgaggcct
    17281 ccgccgacga cgaccacgtc ggcgtcggtg tccccggaca gcgacggccg gggggtcagg
    17341 tcgtcgccgc aggtctcgag ccacaggctc cgggaccgca cgcgctcgac gtcggggacg
    17401 gtcaccgggt gagctcggcg gcgaaggtgg cgaaggcgtc gacgtagcgg tcgacgtgga
    17461 cttcggagtg ctgcaccgac agggtccact gctcctcgtc gccgggggtc atgaacacgc
    17521 cccggttgac catccacggg tagctggcgg tgtagaggtg gatgttggtc tcgaggaagt
    17581 cccggtagca ggtcagcggt tcggcgcggt acgagatgca ccccttgcag cccaggtcga
    17641 cggtgtgggc cggcaggtcg tactcggcga tcagcgcgtc catcccgttc gagaggcggg
    17701 cgccgagcgc gtcgaagtgc tcgtacgcgt cggcggtcag gacccgggtg agggtggcga
    17761 gcgcggcggt gaccgacatg gggttgccgt tgaaggtgcc ctggtgggcg gctccccggt
    17821 tgatctcctc catgatgtcg gcccggccgc cgaacgcgcc gatcggggcg ccgccgccga
    17881 tcgccttggc ccaggtcgcc agatcgggcc ggaccccgaa gcgctcctgg gcgccgccct
    17941 cggcgatggt ggcgccgcac ttgacctcgt cgaagatcag gaccacgccg tagcgggtgc
    18001 acaggtcccg gacggcctgg aggtagccgt cggcgggcac gacgatgccg atgttcatca
    18061 tcaccggctc catgatcagg cacgcgatcc ggtcgccctc ggccgccagc agccgctcca
    18121 gcgcggcggc gtcgttgaac ggcaccaccc ggatccactc ggccaggccc tcgggcatcc
    18181 ccttggactt gggggtcgcg gcgggggcgt cgcggccacc catgacatcg gcgccgggca
    18241 gcaccgagta catgagctgg tcgacgtggc cgtggtagct gccctcgatc ttggcgatca
    18301 cgtcgcggcc ggtggcggcg cgggccacgc ggatcgccga catgttggac tcggtgcccg
    18361 agttggtgaa ccgcacctgt tcgacgcgga accgccggca cagctcctcg gccaacgcca
    18421 ccgtctgctc gacggtcacc gcgaagtgca tgccgttgcg cgccgcgtgc tcgaccgcct
    18481 cggccaccac cgggtgggcg tggccgacgg ccatcgaccc gaacccgttg tggaagtcga
    18541 agtaggcgtt tccgtcgacg tcccacacct cggcgcccac gccgcggctc aggtacacgg
    18601 ggtaggggtc gccgagctgg aagctcgacg ccacgccgcc gggcatggag ccgacggccc
    18661 gttggaagag ggcttccgat ccccgggtcc gctcgaggag gcggggcatc tcgaccgccg
    18721 cgatctcctc ggcccgacgg gtcagctggt cgtctcggtc ggtgcccatc agcccttcac
    18781 ccctcaccac ttcaccatga cgtgcttgag ctcggtgtag tgctcgaccg cgtactgcga
    18841 catgtccttg ccgtagcccg actgcttgaa gccgccgtgg ggcatctccg acacgagcgg
    18901 aatgtggtcg ttgacccaca ccgtcccgaa ccgcatcagg gcgctggcct ccatggcctg
    18961 accgacgtct cgggtgaaca ccgaggcggc gagtccgtag tcgacgtcgt tggccatggc
    19021 gaccgcggcg gccaggtcgg gggcggattg cacggtgacg accgggccga acacctcgcg
    19081 ctggacgatc tcggcgtgct ggtcgacgcc gacgatgacc gacggctcgt agtagaagcc
    19141 gtcgccggat ccgagcgccc cgccggtcac cgcggtcgct cccgcggcga cggcgcggtc
    19201 gacgaagccc tgcacccggt cgcgctgcgc ggcggagatg accgggccga gctcggtcgc
    19261 ctcgtcggtg gtcggtccga cgaccaggct gcgggccgcc tcggagatgg cgtcgaccgc
    19321 ggcgtcgtgg atgccggcac cggtgatgac gcggcaggcg gcggtgcagt cctggccggc
    19381 gttccagtag ccgccgacct tgatcgactc ggcgacgacc tccaggtcgg cgtcgtcgag
    19441 caccacgacg ggggccttgc cgccgagctc gaggtgcacc cgcttgagcg actccgacgc
    19501 ggcgcgggcg atcgccttgc cggtcccgac cgacccggtg agcgagatca tctcgaccag
    19561 cgggtgatcg accagggtgg ctccggtctg cttgccgccg gcgacgacgt tgagcacccc
    19621 ggccgggaac acgtcagccg cgatctcggc catcagcagt gcgctcatcg gggtgagctc
    19681 ggagggcttc agcaccacgc agttgccggc ggccagcgcc gggcccagct tccacgccgc
    19741 catcatgaac gggtagttcc acggggcgat cgacgcgacc acgccgaggg gttcgcggcg
    19801 cagcatcgac gtgtagccct cgaggtactc gccggccccc tgggtctcca tggtccgaca
    19861 cgcgccggcg aagaagcggt agcagtcgac cgacgcctcg atctcgacgc cgaccgcggc
    19921 gatcggcttg ccggcgttgc ggtgctcgac ggcggccagg tcatcggccc gggcctcgat
    19981 cgcgtcggcg aagcggagca gggcggccat gcgctcggcg ggggtggtgc ggccccaccc
    20041 gccgaaggcg tccgcggcag cccgacacgc gtcgtcgacg tcatcagctc cggagtccgg
    20101 agaagtgaaa accaccgatc cggtggccgg gtcgacgatg tcgatcgtgg cgccggatcg
    20161 ggccggtgcc cacctcccgc cgatgaagtt ctggttgcgg acctcgatgt cagccatcgg
    20221 ggtgacctcc tgcggtaagc atgtgtcgac aacacggtgc gggtgccggt gacgatccga
    20281 cccgatgtcg ggaatcgttg cagtaaggta ccaaaccact cggaatccat ggctgtgcgg
    20341 ggggaccgcg acgaatgtca gagccgacgg gaatggcaga gccgacagga acggcagagc
    20401 gcaacggcgg cgggtcggca gtggtgcgcc tggagcaggt caccaagcac ttcggcgact
    20461 tcgtcgcggt cgaagaggcc gacttcgaca tcggcgaggg cgagttcttc tcgatgctcg
    20521 gcccgtcggg ctgcggcaag accaccacgc tgcggatgat cgccggcttc gagcagccga
    20581 ccaccggtcg catcctcctg cagggccagg acgtgtcgcg caccgcgccg cacaagcgcg
    20641 acgtgaacac ggtgttccag aactacgcgc tgttcccgca catgaacgtg ttcgacaacg
    20701 tcgcctacgg ccttcgcaac aagaaggtca aggaggccga ggtcgccacc cgggtcaacg
    20761 agatgctcga ggtggtgcgg ctgcccgagt tcgcccagcg caagcccaac cagctctcgg
    20821 gcggccagca gcagcgcatc gccctggccc gggcgctggt caacatgccc aaggccctcc
    20881 tgctcgacga accgctcggc gctctcgacc tgaagctccg ccaggtcatg cagctcgagc
    20941 tcaagcgcat ccagcgcgac gtcggcatca ccttcatctt cgtcacccac gaccaggaag
    21001 aggccctgac catgtcggac cgcatcgcgg tcatgaccca gggccgggtc gaccagatcg
    21061 gcacgccgac cgagatctac ggcaaccccg ccaccacgtt cgtggccgga ttcatcggaa
    21121 ccgccaacct gctgccggtc agcgtggtgt cccagaccgg cgaccaggtc accgtcgact
    21181 cccggctcgg cacccagttc cgtgtgccgg ccggcggtcg cagctacggc accggcgccg
    21241 acgccacgat catgatccga ccggagcgcc tggtcccggc cggggcgccg ctgcacggcg
    21301 gcgaggccgc cggctcgatc gagtgcacgg tctccagcct ggtgttccag ggcccggtcg
    21361 tgcggtgcca ggtccagacc cgcaccggcg ccgacctggt cgcccacctg ggccccgagg
    21421 acgacccggc cagcctgtcg gtcggttcgc cgatcaccat gacgtgggcc tacgagggcg
    21481 cctacctggt ccccgaggcc atcgcggccg atcaggccgg cgtgacgtcg atggacgagg
    21541 acgagaccga ggcggtgtcg gtcggctgag ccgacggccc gtcatacgag accgagccgg
    21601 ccccgagccg gcgcggatgg acccggctga tgccggccga tcaggaggat cccatggcga
    21661 accgagtgac ccgccgacag ttcatgaccc gctccggcct ggccgttggt ggggcggtga
    21721 tcgctccgca gctgctcgcc gcgtgcgggg gtgacgacga gagcggcgga ggctcggacg
    21781 cctcggccaa gggcgtgtac ttcgagaact ggccggagta catcgacacg gccaaggacg
    21841 gatcggtcga cgggccgggc accacgctgg cgaacttcac cgagcagacc ggcatcccga
    21901 tcaagtacac cgagacctac aacgacaaca acgagtactt cgccaagatc cagccgctgc
    21961 tgtcggccgg caagccgatc ggtccgaacg tgatcgcgcc cacctactgg atggtggccc
    22021 gtctgatcga cctcggctgg ttggagaagc tgccgctcga caagatcccg aactccaaga
    22081 acctggtgcc caacctggtc aagccgccgt cggacccgac cggggagtac tcgctgccgt
    22141 ggcaggccgg caccaccggc atcgcctaca acgtcaaggc gaccggcggc aaggagttga
    22201 cctcggtgga agacctgtgg gacccggcgc tcaagggcaa gatctcgatg ctgaccgaga
    22261 tgcgcgacac gatcggcctc atcgccatgg cggagggcat cgacatctcc accgccaaca
    22321 ccattgagca gtaccagccg gcgttcgaca ccctcaagga gcaggtcgac aacggccaga
    22381 tccgtcagtt caccggcaac gagtacgtcg gaccgctcga ggacgggtcg ttggcggcgt
    22441 gcttcgcctg gtccggcgac gtgctcggca tggagaaccc cgacatccgc tacgtggtgc
    22501 ccgactcggg cggcaccctg tggttcgacg cgatggtcat ccccaagggc gcgtccaacg
    22561 tcgatgacgt cgccgagtgg atgaacttcg tctacgaccc ggtgaacgcg gcgcagatca
    22621 ccgcggcggt gcagttcatc tcgccggtgc agggcgtcca ggagcagctg cgcaagatgg
    22681 gcggcgagga cgcggcgctg gccgacaacc cgctgctgtt ccccgacgac gccaccaagg
    22741 cgcggttgca cacgttcgcc aacctgagcg acgaccagga ggccaagttc gacgaggagt
    22801 tctccaagat ctccggcgcc tgagctcgaa cgaccgggga cgagggagaa ggggagcgca
    22861 ggacatggcc gacgacaagg cgctgcgacg gggccgctgg gccccgatgg gcatgctcag
    22921 tccgggcatc atctggctgc tgctgttctt cctcgtcccg gtggcgatgc tgttcaaggc
    22981 gtcgctgtcg accaagccga gccggttcga gaacccggtg ttcgactgga acttctcgaa
    23041 ctacgcggac gccctcagcc agtaccaacc cgagttcatc cgctcgttcc tctacgcggg
    23101 cacggcgacg gtgctggcca tcgtgatcgg ctacccgctg gcctactgga tcgccacgca
    23161 gggcggtcgc tacaagaacc tgctgatcgg cctggtggtc gtcccgttct tcacgagcta
    23221 cctgatccgg acgatcgcct ggacgtcgat cctgcgtgac gacggaccgt tcgtgagcat
    23281 gtaccagctg ttgccgtggg tcggtgacga cggtcgcatc ctcgccacgc cgtgggcggt
    23341 gatcggtggg ctcacctaca acttcatctc gttcatgatc ctgcccatct acgtgagcct
    23401 ggagaagatc gacttccgcc tgaccgaggc tgcagccgac ctgggatcga cggcgtgggg
    23461 cgcattccgc aaggtggtgc tgccgctgtc gatgcccggc gtgttcgccg ggtcgctgct
    23521 ggtgttcatc cccgcggcgg gtgactacat caacaaccgc tacctgggat cggccaagac
    23581 gtcgatgatc ggcaccgtcg tgcagaacca gttcctggtg caggtcgact tcccacaggc
    23641 cgcagcgctg tcgatggtgc tgatggtgat catcaccgtg ctggtgctca tctactcccg
    23701 gttcctcggc acggaaggac tggccgtatg acgatgtccg gcggggccgc cccgcccgtc
    23761 atggtcgaga ccgcggacct ggatcgggcg cgcaacgccg ggcccatgaa ggtgcggcgg
    23821 cgttggggtc gacgcctgct cacggccttc gcggcgctgg tgttcctgtg gctgttcgct
    23881 ccgttgatca acatcgtgat cttcacgttc aacaagccgg cggggcgacg gaacctgtcg
    23941 tggaacgagt tcacgctcga gaactggtcc cagccgttca aggacaccga cctgaccagc
    24001 gccttcatgc gcagcctgct ggtgtcggcc gtcgcggtga gcgtggcgct ggtgctcggg
    24061 tcgctgatgg ccatcgccct gtcgcgctac cgcttcaccg gatcgaaggt caccgagatc
    24121 ttcctggtcc tgccgctgac cactcccgag atcgtgctgg gcgcctcgct ctaccagctg
    24181 ttcctgggca ccaacacgac gctgggcttc tccaccatcg tcatcgccca catcatgttc
    24241 tgcgtgagct tcgtcgccct gacggtcaag gcccgggtgc gcggcttcga ctggaccctc
    24301 gaggacgcct cgctcgacct ggggtgctcg ccgtggcgca cgttccgcaa ggtcacgttc
    24361 ccgctgatcc tgcccggcat catggccgcc gcgctgctca gcttcgccct gtcgctcgac
    24421 gactacatca tcaccgactt caccaagggc gagtacgtga cgttccccat ccaggtgaac
    24481 aaccagttcc ggatctcgtt cccgccgcag gtcaacgtgc tggccacgat catcctggtg
    24541 gtgagcgtgc tgctgctggt ggtgacctcg gtgcgagagg accgctcgag cgtcgtcgga
    24601 cgctgaagtg cccgcgccgg ggcgcgggtg gtcggtgcgg tcggtgggtc atccgggtcg
    24661 gtcccgcgct caggcaggaa ccggacttcg atgtcggtgc tgggtctcca tggtgagccc
    24721 cgacggggtg gtgatggtga agaacccgtc accttcggct cggtcgtcgt tgggcgacac
    24781 cgaccatccg tcccggtgga ccactccgtg gtggcgccgg cacaacagga cgaggttgac
    24841 catgaccgtg cggccgtcgt cggcccagtg cacgacgtgg tgggcgtcgc accactcggg
    24901 tggatggtcg caccccggga aggcgcaccc gccgtcgcgg acgacgagac cgcggcgcat
    24961 ggccgcagtc gggtggcgga gccggtcgcg gacggcgacg ggcatgccca tggcgtcgag
    25021 gaggacctcg gacaccgagg cgttgcaaac cagcatctcc cactgccggg cggagatggt
    25081 cagccgccga ccgtcggggc tggtgacggg gacgccgaga cgacctccgt gtccgcagca
    25141 cacgtggccg ttctcgtcga agcggtgacg gtgcgggtcg acgtggtgtc cgatgccgtc
    25201 gaggaggccg gccaggatcg ggtccaactc gtccagctcg tcgagccggt cggcgtcgac
    25261 cacgagcgac aggtcggtga cggtcggccg ggtcgccttg gggtcggccg cagcgccacg
    25321 ccggacaagc tcgtgaagcg cctgggcacg gatctgggtc cgggtcggca tctcgaggtc
    25381 ggggttggcg tcgcagtccc gacgccagcg ccgccacagg gcgttggtgg cggcctcgac
    25441 gagctgctcg aacgattcgc cgtcgacgtc gacgtaggtg gccgagagga cgacctcgtc
    25501 gtcgacgcgg tgcagctgtg cggagctggt gacgggatcc ggatcctcgg ctccgtcggc
    25561 gtcggcgaag cgggccaggt cggtgacgag cacgcagaac tgggcgaagg agggctcggc
    25621 ggccgacagg gcgagcagtg cctgctgggc ggcggcgagg gcgtcggcgt tgcggtcgtt
    25681 gagcttggac gccacgtaga gggcgcgttc gccggtgatc tctccgcggg ccagcgccgc
    25741 ggcgatctgg gggaggtcac ggcggagccg gcgcccccgg gacgcctgac gcccgatcgc
    25801 cacccgggat cggccgtggc gccgttcgaa ccacacgcgt gaggtggtcc cgtagcgggt
    25861 gtcgcacagg tcgcggtcgt cgatctcggt cagcaccatc accccgatcg cgtcgatcga
    25921 gcgctgcagg acctcgaggt cgtcgaacac gcccaggcac tcgtcgtcgg tcagtgcggt
    25981 ggcatcggtg gcggcgagca ggcgggcggc cgccagggcg gagtgggagc gggcgatgga
    26041 ccggacctgc tcggggccga agccggtgaa cccggggccg gccgggcgag cggcggtgcc
    26101 gccctccgct cggggagggt ccgcgacgac gtcgtcggga tcgtcgccgt gagtggcgtc
    26161 gtgatcgggg atcgaacaca tgttcgtagt gtgccacgcg gctgtgacac tcggcggtcg
    26221 gggccggaat cggtgaggcg gatcggcgcg acgtggcagg gtgggccatg cgcacgatcg
    26281 aggtcttcgc agatgtgacc tgcccgttca cccacgtggg tctgcgacgg atggtcgccg
    26341 cccggcaggc ccagggccgc gacgacgtgg tcctgcgggt gcggtcctgg cccctcgagg
    26401 tggtcaatgg agccccgctc gacgccgcct tcgtggccga ggaggtcgac gacatccgcg
    26461 cccaggtggc gcccgacctg ttcgtcggct tcaactccga gcgcttcccc tcgtcggcca
    26521 tgacggccat ggcgctggcc gccgcggcgg atcgggtcga cccgatcacc ggcgagcggg
    26581 tgagcctggc gttgcggacg atgctgttcg aggagggcgt cgacatctcc gacgagtcgg
    26641 tgctgcggtc cgtcgcagcg gagcacgggg tggactggtc cgacgtcgat ccggcccgcg
    26701 tgctggctga ccacgcggag ggcatcgagc ggcacgtgac cggttcaccg cacttcttca
    26761 ccccgggcgg tgacttcttc tgccctggcc tggcgatctc gcggggcgac gacggccatt
    26821 tgcacgtcga accgaaggcc gaggtgttcg aggcgttcct cgcctcctgc ttcgcctgaa
    26881 cccggctgcg ttcccgcacg ggacgcggac tgtcagaccc ggttgcttgg atggcgtcgt
    26941 ggccgttcga cacctcaccc ccgtccctgg gcaacccccc gccatcggat tcgccgaggc
    27001 tgtacggacg gtgagcgcgt tggcccgacg ccggggcctc caggtgccgg tgttccggtc
    27061 gccgcccggg ctggacgacg tcgaccggac gctgcggcga cgggcggatg gagcggtcgt
    27121 cgtggccatc cggctcaccg gccgcccgtt cgccgcggtg cagggcgacg tcatcgccgg
    27181 catcgtcgcg gccaatgggc tgaccggccg cgacgccggc cggttccgcc gggcggcctg
    27241 gacggcgttg ggtgacacgc cgggtggtgg gagcgatcgt gccgtcgcgg gctggggcgg
    27301 cccagcggcc agcaccgacg cgaccacacc caatgtgggt gtagcttgag cggggtggcg
    27361 aaggtcgtgc aggtccggaa cgtccccgac cgggtgcatg cggtgctgac ccggcgggcg
    27421 gcggaggccg ggctgtcgct gtcggactac gtcctgcgtg agctcgagct cgtcgcgtcc
    27481 cgcgatgcca acagcgagtt gctccatcag ctcgccctgc tcccgcgctc ggccgtctcg
    27541 ggtgccgacg cggtgaggtc gatgcgtgac gagcgcgacc gcgggatcgc gcgccgtgtc
    27601 gctcgtcctt gacgcttcgg ccgccgtcga gctcgttctc ggtcgtcccg gtctcgcccg
    27661 actcctcctg ggggaggagc tccacgtccc cgctcacttc gatgtggagg tcgtcgccgc
    27721 gctccgtggc gtgcagatcc gtggtgaggc ttcggccgag gacgtggagc gggcaacgaa
    27781 cgcgtttcgc cagctcgtcg tggttcgcca ctgggcgact ctcctcgtcg atcgggcgtg
    27841 ggacctgcgg gatgcgatca cgatccagga cggtgcgtac gtcgcgctgg ccgaaggcct
    27901 gggttgtgcg ctggtgacga ctgatgtccg actggagcgg tcggcgtcgg ggctggttcc
    27961 gatcgtgact ccggacagct gaccggagat cggccgcgcg gagcggttcg tgcgagcatg
    28021 ggcacccgcc cgggtggcgt aactggcagg cgcagagggc ttaaacccct c
//
LOCUS       DAOQOD010000016        27907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_553_length_27907_cov_12.646848, whole genome shotgun sequence.
ACCESSION   DAOQOD010000016 DAOQOD010000000
VERSION     DAOQOD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 27907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27907
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_27907_cov_12.646848"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..459)
                     /locus_tag="PLZ72_03215"
                     /pseudo
     CDS             complement(151..459)
                     /locus_tag="PLZ72_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            513..1973
                     /locus_tag="PLZ72_03220"
     CDS             513..1973
                     /locus_tag="PLZ72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNA35341.1"
                     /translation="MKGSFRGPYAEGPPPSALEVDEPRLETFRPADLWRTVVAVVVVS
                     WSVLRALLGRLIGRRSTPVVQTACDGLIDAFIHLGPTFVKSGQIIASSGGMFPEVLAN
                     SARRCLDEVPPFPVEQVRSTIAEDLGAPVDEVFASFDDEPLSAASIGQVHACTLRDGR
                     TAVVKVQRPDIRDQMATDLRNMFRIARFIEWTPWGRTSGAKGIIRDLHSVTFRELNPA
                     LEAFQQDRFRHNIGAFGDNTMITAPEVYWDHCGPRTICMQRVHGIPMDQFDEMVARGM
                     DGQAILRRGAKVWAEAAMVHGPFHGDMHAGNIWALDDGRGCYLDFGIMGELSLEWRGV
                     LKDLFYTCAFDLDFTRVARAYREVGAIPPGMGSDEELGAFLNMVLGPMLADGFGSIDV
                     AALVAQSTEMLKQYNASIPQELALIAKQLLYIDRYTKFLAPDYSITADPYIVQNIFPD
                     EARAKAAELGVVLDDTDPAFNTPLPPPGDLEHAAEA"
     gene            complement(2020..2946)
                     /locus_tag="PLZ72_03225"
     CDS             complement(2020..2946)
                     /locus_tag="PLZ72_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNA35342.1"
                     /translation="MNLRELEYLVAIADHRHFGRAASSVFVSQPTLSTQLKKLESELG
                     VQLVERSRGGVLLTAAGQAVVERARVVLRETADIRDIARRAADPSAATLRVGLFPTLG
                     PYLLPHVVPHVRERMPRVELLLVEEKTEVILQQLADGTLDVGILALPVDDGRLEQVDL
                     FTEDFLLAVPADHPLASRREPIEATALAGEQVLLLEEGHCLRDQALSVCQVVGASERR
                     GFRATSLETLRQMVAAGVGMTLLPELAVRAPVPTQDDIALLRFADPVPTRRIAMFWRP
                     TNTYAEILPELAAVFRDLPDDYVRVATPSGPT"
     gene            complement(3007..4629)
                     /gene="ahpF"
                     /locus_tag="PLZ72_03230"
     CDS             complement(3007..4629)
                     /gene="ahpF"
                     /locus_tag="PLZ72_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNA35343.1"
                     /translation="MLDDAIATQLRTHLQKVVQPIVLRASLDDGAKSAELAALLDEIA
                     AMSELITVERTDDDERRPSFAIVRAGTDVSVRFAGIPLGHEFTSLVLALLQVGGHPST
                     ARDETVRAVEALTDDLHFETYFSLSCQNCPDVVQALNLMSVLNPRVSHTAIDGALFAD
                     EVESRQVMAVPAVFLNGEPWGQGRMTLEEIVDRLDSGAAERRAEELADRDVFDVLVVG
                     GGPAGAAAAIYSARKGISTGVVAERFGGQVLDTLAIENFISVPYTEGPKLAAAMEQHV
                     LDHDVDVMDLQRAARLIPGSDGDPIGVELDNGVVLRSRSVVISTGARWRTMDVPGEAE
                     YRNKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVSHVTLIEFDDQLRADD
                     VLQRKLRSLPNVDVLVSSLTTEVHGDGSTVTGITVRDRVEGSDRLIELDGVFVQIGLL
                     PNTEWLEGTVELSPRREVVIDDHGRTSVPGVFAAGDCTTVPFKQIVIAMGAGATASLS
                     AFDHLIRTGAPAEDGSPTTAPAAAADLAAAPS"
     gene            complement(4746..5309)
                     /gene="ahpC"
                     /locus_tag="PLZ72_03235"
     CDS             complement(4746..5309)
                     /gene="ahpC"
                     /locus_tag="PLZ72_03235"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNA35344.1"
                     /translation="MSLVNTEVLPFTATAFHNGEFVDVSEADFKGKWSVVFFYPADFT
                     FVCPTELGDMADNYEEFQRLGVEVYGVSTDTHFTHKAWHDTSDTIGKIRYPMIGDPTW
                     QLSENFQVLRPGQGLADRGTFLVDPDGVIQFMEVTAEGIGRNAAELLRKVRAAQYVAA
                     HPGEVCPAKWEEGEETLAPSLDLVGKI"
     gene            complement(5461..9165)
                     /locus_tag="PLZ72_03240"
     CDS             complement(5461..9165)
                     /locus_tag="PLZ72_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNA35345.1"
                     /translation="MSPDEPDPLRLRTIPTDRSPSPRRATWIVLLVVLVTGSAAAAVA
                     WRIADVAAEDKRSEAVESVLRSIERDTDDLVVAMSGTSALVQADGTITQGALDRYATD
                     LAAVGQARPLVWMTPVAADSWEIRLAAVGRGPDPRPDDDAAALDGLEAGRVLGADSPL
                     AAAANSARELGRPTLARVDSGRGARLVVLKPLYLIGDGEPEFIGVAASADLPGQLPSR
                     VATDVPADVRFRVADGDTVLAVSDPPPTAGVTTDVAVAGRILSVQVEDARPVNHDLSW
                     FLLWIMAVIVAAAGVVGLRSARYDQDRRLTNATISRTAALAQRLARAATVDEVADVIG
                     DHVPALLGADVASFGEVDGDVVRLHHRSGTDPAVAELTELPLDGIPSLVREVDAGHTV
                     LLRDPQEWRRAMPGGIADRFLDAGARVAAVLPLEAPGRGVVATIGILWWRPPELDERT
                     TATLDTVRELCEQSLSRAALTDRVSERASALADLAERLAGADTVVEVARTASSGAARV
                     VGARTVDIGVTDVDRGVMRLYRDPRALGPDEPVVSELGLDADTAQTAAARTGAPDLQP
                     RPVEDPDGSPAGARAAFPLRADERVVGAICWSWDRPVAFDPDTVNELTTVAEITAQSI
                     RRAQLIEDQAADARRNRALAELAQGLAARSETEDIAGFLTESVMRPLGAAYSVVGVID
                     GDRIVRRYSESLVRTGLTALGQDQRSVGIGSPTPDAEAARTASAVVVTDRTQADDRYP
                     TMIGVWDQLGVQSGAAVPVKDRNGRVVAVISVLWDHPTVIRPDVSDTLATVAGMVGQT
                     LERTGLVDELRRSVLRNQRLADFARLLADVRSVDEIRKRVRSDGAAPVGADVIDMVLL
                     ADGRSVAGPERTDGPGLLTDGTGASSTTLVEPARECLRQREMVTLGASAIRARLPTST
                     VEELSSTGIVLVAYLPLVAPDGAELGVVALGWPGPPDLTPTVVAKLRTVAELCSQALQ
                     RTRLREAEHRLVVSLQERVVHPVPDAPGLLIAQRYLPAAEQVGMGGDWFEGVELGAGR
                     FAVIIGDIAGHGINAVADMVELRAIIGSMLRSGTPLEEVYPRVAALVQQGGRGLTATS
                     CTAVFDTSDDTVRYVSAGHLPPVLARPDGSVELLEQGRQPLLGVTGSIVAPGVAPFGP
                     GSSLVLYTDGIVERRREPIDVSIENLRVATTELVRTHLHRGTEVTTPTQDDVERFTDA
                     LLRSCLGERSTDDDVALVVVTRADRIATGR"
     gene            9269..10105
                     /locus_tag="PLZ72_03245"
     CDS             9269..10105
                     /locus_tag="PLZ72_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA35346.1"
                     /translation="MEWSDQDDRGRPDGRRSGTGRRARSARRCAAAELTAEEVGALVD
                     EYHRSGDRRLRNQVVEAHLWVAEHQVRAVRHAAGASVEDLRQTALLAMIRAVDRFDPS
                     RGVTFRTFAARTIEGELKRFLRDRSWAVRPPRRAQELNLIVRRRTDELAQSSGRSPTV
                     GEVSASLDVPVDDVLAALEAGRARSAVGLSAPARDPVDRRPILERIGAPDPALASVDD
                     RCLVQSAVDGLDLRERQVLQLRFVDDLSQPEIAAAVGVSQSYVSRLLRAGLDHLREKL
                     AS"
     gene            complement(10181..10960)
                     /locus_tag="PLZ72_03250"
     CDS             complement(10181..10960)
                     /locus_tag="PLZ72_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA35347.1"
                     /translation="MSPDEVGGAGEAAGAGEAAPAPIDPRLVEYARTHDRALRNALVE
                     DNMGLALHFTTRYRRSDTEDDDLRQVALLGLVKAIDRFDPTRGVAFSTFAGRTIDGEL
                     KRHFRDQTWALRVPRSVKDLHVSVRRASEALEQQLSRSPTVRELADHLDVSVDEVIGA
                     LGATAARRAGSLDDLDPQGATTTPRTVSSPAEDLLEDRDEVGRLLSTLGPREREIIRL
                     RFYEHLSQEQIAERVGLSQMHVSRLLRRSFEQMRRSGTPPS"
     gene            complement(10983..11369)
                     /locus_tag="PLZ72_03255"
     CDS             complement(10983..11369)
                     /locus_tag="PLZ72_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35348.1"
                     /translation="MSEHPTAESGPPQRTVGLEIPAEFRFVSMARVAAASLAAELDPA
                     IDDLEDLRVAVNELVGILVEAAEGGTILIRMWIEERTIHVSGRCLGEWAPTEPDQLAQ
                     RILDATVDHHEIGDGAFRMLTHLSAE"
     gene            11615..12496
                     /locus_tag="PLZ72_03260"
     CDS             11615..12496
                     /locus_tag="PLZ72_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNA35349.1"
                     /translation="MFANFLIGLREGLEASLVVGILVAYLVKTDRRDQLPKVWLGVGI
                     AVVISFAFGAALTFGPRGLTFEAQEAIGGTLSIVAVGFVTWMVFWMAEQARNIKGELE
                     TRVDSTAGGVGLVVLALLAVGREGLETALFLWAATRATGDTLAPLLGALLGLVVAVVL
                     GVLVYRGALRLDLRKFFTWTGGFLVVVAAGVVSYGVHDLQEAAILPGLHNVAFDVSGT
                     IPPGSWYGTLLKGTVNFNPRTTWLQLVCWLLYLVPTMTLFIIRVRRPSGPVTSTLVAA
                     TPDQTHPSPTITEGHPA"
     gene            12493..13680
                     /locus_tag="PLZ72_03265"
     CDS             12493..13680
                     /locus_tag="PLZ72_03265"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M75 family protein"
                     /protein_id="HNA35350.1"
                     /translation="MTPTVRRLLAPSLIAVVALAGACTDNTKEAASGSDDGGGKGPTT
                     IEVTDTGSECTLSATEAPSGNIVFSVTNKGSEITEFYLLAEDGKSIIGEVENIGPGLS
                     RDLVLRAEPGSYVTACVPGMTGDGHRGDFTVTDSGETDATSGEMKALEAAATKQYKDY
                     VTQQVGFLVAGTQKFADAYKAGDDDQARALYAPTRAYWESIEPVAESFGDLDPRLDLR
                     EADLEAGQEWTGWHQIEKQLWPPADFTEIDTPEERAKMADQLVADTQDLATRVETVTF
                     KADQLGNGAKELLDEVATGKVTGEEEFWSHTDLWDFQANVDGARLAYQALRPIVLIKD
                     KDLAATLDEEFASLQTELDKYKTADGFQYYTQLTPEQVKELSDSVNALGEPLSQLTAV
                     VVL"
     gene            13741..15111
                     /gene="efeB"
                     /locus_tag="PLZ72_03270"
     CDS             13741..15111
                     /gene="efeB"
                     /locus_tag="PLZ72_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HNA35351.1"
                     /translation="MDDDTTGETVERDGSTADLATASGDGAGADEPDRSGVSRRWLLG
                     AGAVGAAAVGVAAGATIASASEPDSDPSEGAAAAAVPFRGEHQAGIVTPAQDRLHFAA
                     FDVITDDREKLVELLQKWSAAAERMTEGHSAGTTGAMNGPLDAPPADTGEAYGLPPSQ
                     LTITIGFGPTLFTKDGQDRFGLAERRPEALIDLPHFPADRLDPARSGGDLCVQACAHD
                     PQVAVHAVRNLARIGFGVVSVRWSQLGFGRTSSTSTAQDTPRNLFGFKDGTANLKAEE
                     PDLLDEFIWVGKDDDPAAAWMAGGSYLVSRRISMHIETWDRTSMREQEMIFGRDKGEG
                     APLSGGTEFTEPDFDAEGPDGEPKIALMSHVRLAHPDDNDGVQILRRGYNFTDGTDGL
                     GRLDAGLFFIAFVRDPRTQYVPMQTKLSRDDLLMEYLVHTGSGLFAVPPGVTGPGRWV
                     GQDLFA"
     gene            15264..16523
                     /locus_tag="PLZ72_03275"
     CDS             15264..16523
                     /locus_tag="PLZ72_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA35352.1"
                     /translation="MTEVAISTDPRGRIFITPEAFADMDAWHATAAEIRRDEPVMRVE
                     AEGWTPFWAITKHADVFEVSRRSDVFFNTEKSAPGPDLQYDMLQAFGIELPRTLVHIH
                     GDVHNKLRSVTNDWFKPAAVRHLQPTIESIADEFVDKMAAMDGRCDFAADVAQPFTIR
                     VIMSIFGVPQSDEPLMLSLTQGLFGAADPEYMGDFSDPLAAITSSINRFEEYFDALSD
                     ERRENPTDDLATVIANGMVDGAPMGDRERLWYYIIVATAGHDTTSFALSGGLERLLAH
                     PDQLRAVQDDPTLAGAAAEEMIRWTTPVRSFMRWAQADTEIGGVPIAAGDAVLTSYPS
                     ANRDEDVFVDPMRFDIGRPDADQLLSFGLGMHYCLGSQVARREVRTLLAKVLERVDTI
                     ELDGEPAWSGAHFVSGVKHLPIRYTLR"
     gene            16561..17829
                     /locus_tag="PLZ72_03280"
     CDS             16561..17829
                     /locus_tag="PLZ72_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA35353.1"
                     /translation="MPEGTTARAAGSAGFPDGFMWGTGASSLQTEGAAPRSDWYAWEA
                     AGRAPRSGDGNGFGVRYQEDFAQLAELGLTHHRLSLDWARLEPHPGRHDPAAVEHYRA
                     VLTAARDAGISVWVCLHHFDLPGWFSEDRGGFLEPEARSRTWPRHVDWVGETFGDLVH
                     GWMPVNEPVAYAMLGWLLGTIPPGRRDPERFSEALEAVLLAEHEAWRVLRSGDQPTCT
                     IHSLMPVFAATAGEDERRTAAARANAELVDEVVWRTWIRAMRDGVLHVPGRGLIEVPE
                     MAGAFDLIGFSYYHALGVTADNNFVPHPAGIRPGPHGLPPWAEGLRICLERLADDLPG
                     RPLVVSECGLGTWTAEDDDEQRCQYLTDCLEITRDAVADGIDVRGFFHWTGVDNYEWG
                     LGFDAAFGLIDRDRSPKPSAELARRWATGR"
     gene            complement(17848..19344)
                     /locus_tag="PLZ72_03285"
     CDS             complement(17848..19344)
                     /locus_tag="PLZ72_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNA35354.1"
                     /translation="MRDEASPDERIRRGLRELREELALPREFPPEVLAAADLAARAGG
                     LAGDTAAGRRRRDLTGIDFVTIDPPGSTDLDQAVHIERRGQGFRVRYAIADVAAFVGV
                     GGPLDDECRARGVTIYLPDGRIPLHPPVLSEHAASLRAGEDRPALVWQLDLDADGSAD
                     DVVLERAVVRSRAELDYPSLQRSLDDGDPATRPEVATLLAEVGRLRQDRAAERGAVSL
                     PLPDQEVVIDADGHIGLTFRAPLPVEDWNAQISLMTGMAAARIMLDAGVGVLRTLPPP
                     DERTVAELRADAAALGVCWEPEDSYADVIRSLDPAFPDQAAFATQAARLLRGSGYAAF
                     DRRAGIAPPEGSDAEHSALAAPYAHVTAPLRRLVDRFANEVVLACCAGAEVADDIRAA
                     LAVLPELMASARRRESQADSGARDLVEQVILSGHVGQPLDAVVVGVDRHGLRLQVRDP
                     AVLGRVDTATAASFHLGQEVVVTADDAGSLELSAPSSDRHADGREVEA"
     gene            complement(19432..20826)
                     /locus_tag="PLZ72_03290"
     CDS             complement(19432..20826)
                     /locus_tag="PLZ72_03290"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA35355.1"
                     /translation="MSSPVPTTSTASTAPAAGPTAATSPFWGHFTAMGDDPLPVMVRG
                     EGCYVWDRAGNRYLDGLSGLFTVQVGHGRRELAAAASEQMSTLAYFPIWSYATEPALA
                     LAERLTGMAPGDLDRVFFTTGGSEAVDTAWKLARQYFKAVGQPDRTKVISRDLAYHGT
                     TMGALSITGLPAIKEPFEPLVPGAVKVRHTSSFRSGHDFDEEWGEICARDIEDRILME
                     GPDTVAAVYLEPVQNTGGTLPPPPGYFARVREICDRHGVLLVSDEVICAFGRLGEWFG
                     SDRFGYRPDMITFAKGVTSGYSPLGGVLVSDRLAQPFVEAHSTFLHGITFGGHPVSCA
                     VAMANLDLMEHEDLLGNVSRRASGFQERLEGLLDLPIVGDVRGDGFLRTVELVRDPDT
                     LETFTDEECAWLLKGQISHRLFESGLMCRSDDRSDPMILLSPPLIAGDDELDLIESVL
                     RSVLTEAWTSLQHR"
     gene            20942..21748
                     /locus_tag="PLZ72_03295"
     CDS             20942..21748
                     /locus_tag="PLZ72_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA35356.1"
                     /translation="MDHRYDDFPHLQFDRPSDGVLRITLDGPGLNAVDADVHRELADV
                     WLAVDRDPDTRVALLRGAGRAFSAGGSFELLDGMIEDFAVRTRVLREARDLVFNVIDC
                     SKPIVSALHGPAVGAGLVAGLLADVSVAARTARIIDGHTRLGVAAGDHAAVCWPLLCG
                     MAKAKYYLLTCDTLSGEEAERIGLVSICVDDDQVQDRALAVAEQLAAGSASAIRWTKH
                     ALNNWYRDHSSIFDASWGLEFYGFGGPDAAEGLASHREKRAPEFRGPTSE"
     gene            complement(21768..22958)
                     /locus_tag="PLZ72_03300"
     CDS             complement(21768..22958)
                     /locus_tag="PLZ72_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNA35357.1"
                     /translation="MSDRPPPDPFAPARLGPLTLRNRTIKAATFEGVMRNGAVSDELI
                     EFHRRVAAGGVGMTTVAYCAVSRDGRVQRHTLVMEPERVPDLTRLTEAVHSEGAAIQA
                     QLGHAGLVANTRSNGMPTLAPSTRFSAPAMARVRGATPEQLDRVVDDFATATRVAADS
                     GFDSVEVHLGHNYLLSSFMSPNLNRRTDRYGGSLENRSAFPRRVLAKVRETAPDHMAV
                     TAKFNMADGVDAGLWLDESLPIAQMIEADGHLDALQLTGGSSLLNGMYFFRGDVPLKE
                     FRASQPAVVGFGLRFYGPKLFPHYPFEEGFFLPFARQFRDELKMPLVLLGGINRRDTV
                     DQAMAEGFEFVAMARALLREPDLVNRMQSEPRHEGLCVHCNKCLPTIYTGTRCVLVEP
                     SATT"
     gene            23009..24448
                     /locus_tag="PLZ72_03305"
     CDS             23009..24448
                     /locus_tag="PLZ72_03305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNA35358.1"
                     /translation="MPIRDERRLPEESCLPAGRPDIDRLGTGELPDDRWCALVAAELS
                     PDLLVVIDPLLRAVWTNAAVERLLGLQRGEMLGDPVSDHVHPDDLMVAVGALNESQRN
                     DGYHVATRIRVRRRDGSYIDTRVTATTITDESGTWMVLALRPVEDEVAIERRRAQLKA
                     LAQSVYVECAAVHWYELGDKIPAILGALAGVVGAHRVEVVEPRDGSFERVAGWSARGS
                     TSCGSGRVQLLADPERLRAAPCVVTTVEPGGTGDRPDCGCGVDVEPTHRMVVELWLDV
                     DGVVRLLFDEYTETWDDANADIIALMCSTMLATMRRCEQESELNIRATRDPLTRLLNR
                     TALHQRLGEMLAGMDPARPPIVLFADLNRFKQLNDRFGHREGDAVLCRVADAISSQVR
                     SDDVAARMGGDEFVIVVAGDDVPVATMVSRVRASIDAALVDWPDVGVAVGAIVAEVGE
                     SPEDLLDRADRAMYDDKARSRSARSVART"
     gene            complement(24564..24752)
                     /locus_tag="PLZ72_03310"
     CDS             complement(24564..24752)
                     /locus_tag="PLZ72_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35359.1"
                     /translation="MPITPTNRFYPLPDGRDLTWSHWPEENATGMIVLERHTDGHLDG
                     SPIGDAQLQELLQLHGVH"
     gene            24862..25704
                     /locus_tag="PLZ72_03315"
     CDS             24862..25704
                     /locus_tag="PLZ72_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNA35360.1"
                     /translation="MDLPPGAEPDPTEATGPSRFGGFRAGFVAVAPLLVGVAPFGLVA
                     GATPVAYGLGADAAVGFSVIMFAGASQLASIEVLGAGGSVLAAVLTACTINLRLILYS
                     ASCSPYLRRLAPARRMVMAYLLTDEAYALSIVRWSEQGSEAQDPPTLRGRWTFYMGAA
                     ASMWATWQVSTLVGILVGNALPSGVRLDVIAPLVFLVLLVPTLVTRAALVAAAVGGVG
                     AVVAAECGAGPMAVVVGSVAGILAGAALDDGADDGAGGGAGGGADGDDGRGQGGPTPA
                     EPLP"
     gene            25701..26018
                     /locus_tag="PLZ72_03320"
     CDS             25701..26018
                     /locus_tag="PLZ72_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNA35361.1"
                     /translation="MSAAWWTILIGAAATFAMRASFLVVAPRMARFPPWAHRLLRQIP
                     PAALAALVLPAFVRPDGQLELWGPRAVAGLVAGIVAWRTRNTLATLVVGMAAAVAFGA
                     VMG"
     gene            26062..27672
                     /locus_tag="PLZ72_03325"
     CDS             26062..27672
                     /locus_tag="PLZ72_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA35362.1"
                     /translation="MVDHVSSEPRTRSWWGWGWEDQALTDAECTALGGLVPGLPDTPL
                     PVPRVTDLDLPAPRAAVPGALDAISDSSPEARAAHARGKAYRDVIRALHGDVGAAPDV
                     VLRPRDEADVVAVLDWASDAEVAVVPFGGGSSVVGGVECPAGSHAGVATLDLTALDRV
                     LEIDRTSRAARIQAGVLGPALEDQLRPHDLTLRFFPQSFEFSSLGGWIATRAGGHYAT
                     LRTHIDDLVESVRMVTPIGVSESFRLPGSGAGPSPDRLVLGSEGTLGVVTEAWMRLQD
                     RPRHRRSTTATFQTAADAFAAVRAVAQSDLNPANCRLLDPAEAALSGAGPGDRFVLVL
                     GFESAVLPVGDRFEMLVELVRDHGGTVAAPRVDGDGSGSERDAVADTWRSAFLRMPYR
                     RDGMARMSALTETFETATTWDRIEELYEVVRHEVGAVIREVTGVDGLVTCRLTHVYPD
                     GAAPYFTVIAAGRRGSELVMWDQVKAAAMDVLSRSRATVTHHHAVGRDHRPGYDRQRP
                     EPFAVALRAAKAALDPAGVLNPGVLIDP"
BASE COUNT         3906 a         9819 c        10146 g         4036 t
ORIGIN      
        1 gccacggccg cagcttccgc gcccgccaga tccgcgccac ggtgtccttg ccgaccccgt
       61 gacgctcggc catcgtccgt gtcgaccact gtgtcgaccc tgacgctccc gaaccttcac
      121 ggctccggca ggccgggggc gggcgaggag ccccgacttc gacgcgcagt aggcggggat
      181 ggcggcgttg ccgaccaggc cctcgatcga gctgataccg accaccccgg ggccggtcga
      241 tgcgctcccc tcgaccgcgg cagcagccgc cgcgacccgc aacggttcca gcagcgcctg
      301 gacgacgaac ggatacgcgg tgaggttgac gtcgacgacg cggcgccagt tctcgtcggt
      361 gagcgacccc agcgggtccg ggctgaccgc gccggcggcg tggacgaacc cgtcgacgcc
      421 gccgagcgcc tcgaccgacc gcgacaccgc ggcggccacc gcggtcgggt cgggttgggg
      481 tcggaggttc ggacatggaa ggctctgcag ccgtgaaggg atcgttcaga gggccctacg
      541 cggaggggcc gccgccgtcc gcgctcgagg tggacgagcc ccgcctcgag acatttcgtc
      601 ctgccgacct ctggcggacc gtcgtcgcgg tggtcgtcgt gtcgtggtcg gtgctcaggg
      661 ctctgctcgg ccggttgatc ggtcgtcgat ccacgccggt cgtccagacc gcgtgcgacg
      721 ggctgatcga cgccttcatc cacctcggtc cgacgttcgt gaagtcgggt cagatcatcg
      781 ccagctccgg cgggatgttc cccgaggtgc tggcgaactc ggcgcggcgg tgcctggacg
      841 aggtcccgcc gttccccgtg gagcaggtgc ggtcgacgat cgccgaggac ctcggcgccc
      901 ccgtcgacga ggtcttcgct tcgttcgacg acgaaccgct gtccgccgcc tcgatcggcc
      961 aggtgcacgc ctgcacgttg cgcgacggcc ggaccgccgt ggtcaaggtc cagcggcccg
     1021 acatccgcga tcagatggcg acggacctgc gcaacatgtt ccgcatcgca cgcttcatcg
     1081 agtggacgcc gtggggtcgt acctcggggg ccaagggcat catccgcgac ctgcactcgg
     1141 tcaccttccg ggagctgaac ccggccctcg aggcgttcca gcaggaccgg ttccgccaca
     1201 acatcggtgc gttcggggac aacacgatga tcaccgcgcc cgaggtgtac tgggaccact
     1261 gcgggcctcg gacgatctgc atgcagcggg tgcacgggat cccgatggac cagttcgacg
     1321 agatggtcgc ccggggcatg gacggccagg cgatcctgcg ccgcggcgcc aaggtgtggg
     1381 ccgaggccgc catggtccac ggtccgttcc acggtgacat gcacgccggc aacatctggg
     1441 cgctcgacga cggtcggggt tgctacctgg acttcgggat catgggcgag ctgtccctcg
     1501 agtggcgggg agtgctcaag gacctcttct acacgtgcgc gttcgatctg gacttcaccc
     1561 gggtggccag ggcctaccgc gaggtcgggg ccatcccgcc ggggatgggc agcgacgagg
     1621 agctcggcgc gttcctcaac atggtgctcg gaccgatgct ggccgacggc ttcggcagca
     1681 tcgacgtggc cgcgttggtg gcgcagtcga ccgagatgct caagcagtac aacgcgtcga
     1741 tcccccagga gctggccctc atcgccaagc agctcctcta catcgaccgg tacaccaagt
     1801 tcctggcccc cgactactcg atcaccgccg atccctacat cgtgcagaac atcttccccg
     1861 acgaggcccg ggccaaggcc gccgagctcg gggtcgtgct cgacgacacc gacccggcct
     1921 tcaacacccc gctcccgccc ccgggcgacc tggaacacgc cgccgaagcg tgagcgaggt
     1981 gggcccgggg tccgtaccgg cttccgggcc ggccccggtt caggtgggac cggacggcgt
     2041 cgcgacccgc acgtagtcgt cgggcaggtc gcggaagacc gcggcgagct ccggcaggat
     2101 ctccgcgtag gtgttggtgg gacgccagaa catggcgatg cggcgggtgg ggacgggatc
     2161 ggcgaagcgc agcagcgcga tgtcgtcctg ggtcgggacc ggtgcccgga ccgccagctc
     2221 gggcagcaac gtcatgccga ccccggcggc caccatctga cgcagcgtct cgaggctggt
     2281 cgcccggaag cccctgcgct ccgatgctcc cacgacctgg cacacgctga gcgcctggtc
     2341 gcgcaggcag tgcccctctt cgagcagcag cacctgctcg ccggccagcg cggtggcctc
     2401 gatgggctcg cggcgagagg ccagagggtg gtccgcgggc accgcgagga ggaagtcctc
     2461 ggtgaacagg tcgacctgct cgagtctccc gtcgtcgacc ggcagggcga ggatcccgac
     2521 gtcgagggtc ccgtcggcga gctgctggag gatgacctcg gtcttctcct cgacgaggag
     2581 cagctcgacc cggggcatcc gctcgcggac gtgggggacg acgtggggca ggaggtacgg
     2641 ccccagggtg gggaacaggc cgacccgcag ggtcgccgcg gaggggtccg ccgcccgtcg
     2701 ggcgatgtcg cggatgtcgg cggtctcccg gagcacgacc cgggcccgtt cgaccacggc
     2761 ctggccggct gcggtcaaca gcaccccgcc gcggctgcgc tcgaccagct ggaccccgag
     2821 ctcggactcc agcttcttca gctgggtcga caacgtcggc tggctgacga acaccgagct
     2881 cgcggcgcgg ccgaagtggc ggtggtcggc gatggcgacc aggtactcga gctcgcggag
     2941 gttcacggcg actccgatcc gtcccgggtc acgcccgggc gacggtcccc gcggcagcga
     3001 tcgtggtcat gagggtgcgg ccgccaggtc cgcggccgca gcgggcgcgg tcgtcgggga
     3061 gccgtcctcg gccggtgccc cggtgcggat caggtggtcg aaggcgctga gcgacgccgt
     3121 ggctcctgcg cccatggcga tcacgatctg cttgaacggg accgtggtgc agtcgccggc
     3181 ggcgaacacc ccgggcaccg atgtccggcc gtggtcgtcg atcacgacct cgcgtcgcgg
     3241 cgacagctcg acggtgccct cgagccactc ggtgttgggc agcagcccga tctgcacgaa
     3301 cacgccgtcg agctcgatca ggcggtccga cccctcgacg cggtcccgga cggtgatccc
     3361 ggtgaccgtg ctcccgtcgc cgtgcacctc ggtggtgagc gacgagacca ggacgtcgac
     3421 gttgggcagg ctgcgcagct tgcgctggag cacgtcgtcg gcccggagct ggtcgtcgaa
     3481 ctcgatcagg gtgacgtggg agacgatgcc ggccaggtcg atggccgcct ccacgccgga
     3541 gttgccgccg ccgacgaccg cgacccgctt gcccttgaac aacgggccgt cgcagtgggg
     3601 gcagtaggcc acgcccttgt tgcggtactc ggcttcgccg gggacgtcca tggtccgcca
     3661 gcgggcaccg gtggagatca ccaccgagcg ggaccggagc acgacgccgt tgtcgagctc
     3721 gacgccgatg gggtcgccgt cggagcccgg gatcagacgg gcggcgcgct gcaggtccat
     3781 gacgtcgacg tcgtggtcga ggacgtgctg ctccatggcg gcggccagct tcgggccctc
     3841 ggtgtagggg accgagatga agttctcgat cgccagcgtg tcgagcacct ggccgccgaa
     3901 gcgctcggcg acgacgccgg tggagatgcc cttgcgcgcc gagtagatcg ctgccgcggc
     3961 accagccggc ccgccaccga cgacgaggac gtcgaagacg tccctgtcgg ccagctcctc
     4021 ggcccggcgt tcggcggctc cggagtcgag gcggtcgacg atctcctcga gggtcatgcg
     4081 accctgaccc cacggctcgc cgttcaggaa caccgcgggc accgccatca cctggcggga
     4141 ctcgacctcg tccgcgaaca gcgcgccgtc gatggcggtg tgcgacacgc gtgggttcag
     4201 gacgctcatc agattcagcg cctggacgac gtcggggcag ttctggcacg acaacgagaa
     4261 gtaggtctcg aagtgcaggt cgtcggtgag cgcctcgacg gcgcggacgg tctcgtcgcg
     4321 ggcggtggag gggtggccgc ccacctgcag gagcgccagc accagggagg tgaactcgtg
     4381 gccgagcggg atgccggcga agcggaccga gacgtcggtt ccggcccgga cgatggcgaa
     4441 gctgggccga cgttcgtcgt cgtcggtccg ctcgacggtg atcagctccg acatggcggc
     4501 gatctcgtcg agcagggcgg cgagctcggc ggacttggct ccgtcgtcca gcgaggcccg
     4561 gagcacgatg ggctggacga ccttctgcag atgcgtcctc agctgggttg cgatggcgtc
     4621 gtcgagcatc tcgttctcct cgctcgtctc aggtcggctc gttgcggatt cgggggtgtg
     4681 cggatcgaca ggacgggccg ggggttctcg ccccccggcc cgtcgcgttc aggggtctcg
     4741 gtgggtcaga tcttgccgac gaggtcgagg gacggggcca gggtctcctc gccctcctcc
     4801 cacttggccg ggcacacctc gccggggtgc gcggcgacgt actgagccgc gcggaccttg
     4861 cgcagcagct cggccgcgtt gcggccgatg ccctcggcgg tgacctccat gaactggatg
     4921 accccgtcgg ggtcgaccag gaaggtgccg cgatcggcga ggccctgacc cgggcgcagg
     4981 acctggaagt tctccgacag ctgccaggtc gggtcgccga tcatcgggta gcggatcttc
     5041 ccgatcgtgt cggaggtgtc gtgccaggcc ttgtgggtga agtgggtgtc ggtcgacacg
     5101 ccgtacacct cgacgccgag gcgctggaac tcctcgtagt tgtcggccat gtcgcccagc
     5161 tcggtcgggc agacgaaggt gaagtcggcc gggtagaaga acaccacgga ccacttgccc
     5221 ttgaagtcgg cctcggagac gtcgacgaac tcgccgttgt ggaaggcggt ggcggtgaag
     5281 ggcaggactt cggtgttgac gagggacatc tggacctctt cgctctgggc ctccgggacg
     5341 gtcccggggg cggtcggtgg ggcgactcgg tgagccgctg ttatagctgt tgacgaacag
     5401 catagcccat gacgattgat catctcgatc gaaacttgct atcggagcga tcgagcccgc
     5461 ctatcgaccc gttgcgatcc gatcagcccg ggtcaccacg accagcgcga cgtcgtcgtc
     5521 cgtcgaccgc tctccgaggc agctccggag cagcgcgtcg gtgaaccgct ccacgtcgtc
     5581 ctgggtcggc gtggtgacct ccgtgcctcg gtggaggtgg gtccgcacca gttcggtcgt
     5641 ggcgacccgc aggttctcga tcgagacgtc gatcggctcc cgtcgtcgct ccacgatgcc
     5701 gtcggtgtac agcacgagcg acgagcccgg gccgaacggg gcgacgccgg gtgccacgat
     5761 cgagccggtc acgcccagca ggggttgtcg gccctgctcg agcagctcga cggacccgtc
     5821 gggtcgggcg agcaccgggg ggagatgacc cgccgacacg tagcggaccg tgtcgtcgga
     5881 cgtgtcgaac accgcggtgc acgacgtggc ggtcaggccc cgcccgccct gctgcaccag
     5941 cgcggccacc cgggggtaca cctcctcgag cggcgtgccg ctccgcagca tcgatccgat
     6001 gatcgcccgg agctcgacca tgtcggccac ggcgttgatg ccgtgaccgg cgatgtcgcc
     6061 gatgatcacc gcgaaccgcc ccgcccccaa ctcgacgcct tcgaaccagt cgccgcccat
     6121 cccgacctgt tcggccgcgg gcaggtatcg ctgggcgatc aggaggcccg gcgcgtcggg
     6181 caccgggtgg acgacccgct cctggaggct caccaccagt cgatgctcgg cttcgcgcag
     6241 ccgggtgcgc tgcagggcct ggctgcacag ctcggccacg gtccggagct tggccaccac
     6301 ggtcggggtg aggtcgggag gtccgggcca gcccaacgcc acgaccccga gctcggctcc
     6361 gtcgggggcg accaacggga ggtaggcgac gagcacgatg cccgtcgagg agagctcctc
     6421 gaccgtcgac gtcggcaggc gggctcggat ggcgctcgct ccgagggtga ccatctcccg
     6481 ctggcggagg cactcccggg caggttccac cagcgtcgtg gaggacgcgc cggtcccgtc
     6541 cgtgagcagc ccgggcccgt cggtccgttc ggggccggcg acggatcggc cgtcggccag
     6601 cagcaccatg tcgatgacgt cggcgccgac gggagcggca ccgtcggagc ggacgcgctt
     6661 gcggatctcg tcgacggagc gcacgtcggc cagcaggcgc gcgaagtcgg ccagacgctg
     6721 gttgcgcagc acgctgcggc gcagctcgtc gaccaggccc gtgcgctcca gggtctgacc
     6781 gaccatgccg gccactgtcg ccagcgtgtc gctcacgtcg gggcggatca cggtcgggtg
     6841 gtcccacagc acgctgatga cggcgacgac ccgaccgttg cggtccttga ccgggactgc
     6901 ggcgccggac tggacgccga gctggtccca cacgccgatc atcgtcggat agcggtcatc
     6961 ggcctgcgtg cggtcggtca cgaccaccgc ggacgcggtc cgagccgcct cggcgtcggg
     7021 ggtcgggctg ccgatgccga cgctgcgctg gtcctgtccc agcgcggtca gcccggtgcg
     7081 caccagcgac tccgagtagc ggcggacgat gcggtcgccg tcgatgacac cgaccaccga
     7141 gtaggcggca ccgagcgggc gcatcaccga ctcggtgagg aacccggcga tgtcctcggt
     7201 ctcggacctc gccgccagac cctgggccag ctctgcgagg gcacggttgc ggcgggcgtc
     7261 ggcggcctgg tcctcgatga gctgggctcg acggatcgac tgggcggtga tctcggcgac
     7321 cgtcgtcagc tcgttcacgg tgtcggggtc gaaggccacc ggtcggtccc aggaccagca
     7381 gatggcgccc accacccgct cgtcggcgcg gagcgggaag gcggcgcggg ctcccgcggg
     7441 gctgccgtcg gggtcctcga cgggacgggg ctgcaggtcc ggcgctcccg tgcgggccgc
     7501 tgcggtctgt gcggtgtcgg cgtcgagccc gagctcggac acgacgggtt cgtccggccc
     7561 gagggcgcgt gggtcgcggt agaggcgcat caccccgcgg tcgacgtcgg tgacgccgat
     7621 gtcgacagtc cgggccccga cgacgcgggc cgcgccggag gatgcggtgc gcgccacctc
     7681 gacgacggtg tcggcgccgg ccagacgctc ggcgaggtcg gccagcgccg aggcgcgttc
     7741 ggagacgcgg tcggtgagcg cggcacgcga caggctctgc tcgcacagct cgcgcaccgt
     7801 gtcgagcgtg gcggtggtcc gctcgtcgag ctccggcgga cgccaccaga ggatcccgat
     7861 cgtcgcgacg accccacggc ccggagcttc gagcgggagc accgccgcga cccgggcccc
     7921 ggcgtcgagg aacctgtccg cgatcccccc gggcatggcc cggcgccact cctgcggatc
     7981 gcgcaggagc acggtgtggc ccgcgtcgac ctctcgtacc agggagggga tcccgtccag
     8041 ggggagctcg gtcagctcgg ccaccgccgg gtcggttccg ctccggtggt ggaggcggac
     8101 gacgtcgccg tcgacctcgc cgaacgacgc gacatccgcg ccgagcagtg cgggcacgtg
     8161 gtcgccgatc acgtccgcga cctcgtcgac ggtcgcggcg cgggccagcc gctgggccag
     8221 ggccgccgta cggctgatcg tcgcgttggt gagccggcgg tcctggtcgt agcgggcgct
     8281 gcgcaacccg accaccccgg ccgcggccac gatgacggcc atgatccaca gcaggaacca
     8341 gctgaggtcg tggttgaccg gacgggcatc ttcgacctgc accgacagga tccggcccgc
     8401 gaccgcgacg tcggtggtca caccagcggt gggcggtggg tcgctgaccg ccaggaccgt
     8461 gtcgccgtca gcgacgcgga accggacgtc ggcggggacg tcggtggcga cccgcgacgg
     8521 cagctggccg ggcagatctg ccgaggcggc gaccccgatg aactcgggct cgccgtcgcc
     8581 gatcaggtag agcggcttga gcaccaccag ccgtgccccc ctcccggagt ccacacgagc
     8641 gagggtcggt cgaccgagct ctcgggcgct gttcgccgcg gcggccaggg gggagtcggc
     8701 tccgaggaca cgacccgcct cgagcccgtc gagcgcggcg gcgtcgtcgt ccggtcgtgg
     8761 gtcggggccc cgacccaccg cggccaggcg tatctcccag gagtcggcgg cgacgggggt
     8821 catccacacc agcggccggg cctgacccac cgcggccagg tccgtcgcgt accggtccag
     8881 ggcgccctgc gtgatggtcc cgtcggcctg gaccaacgcc gaggtgccgc tcatcgccac
     8941 caccaggtcg tcggtgtcgc gctcgatgga gcggagcacg gactcgaccg cttctgagcg
     9001 cttgtcctcg gcggccacgt ccgcgatgcg ccaggcgacc gcggccgcag ccgatccggt
     9061 caccaggacc acgagcagca cgatccaggt ggcgcgccgg ggtgacggcg atcgatcggt
     9121 cgggatggtc cgcaaccgga ggggatcggg ttcgtcgggc gacacggcgc ctcgaacgct
     9181 aacgggcgcc aggatgatcg ggaaggtcgt cgatgaaggc tgaccggacc cttccgccgg
     9241 acgtgacggc ggaccatgat ggatcacggt ggagtggagc gaccaggacg acagaggtcg
     9301 accggacgga cgtcggtccg gtaccggtcg acgcgcccgc agcgcccgac ggtgtgcggc
     9361 ggcggagctg accgcggagg aggtcggggc gctggtcgac gagtaccacc gcagcggcga
     9421 tcgccggctc cgcaaccagg tggtcgaggc gcacctgtgg gtcgccgagc accaggtccg
     9481 tgccgtacgg cacgccgccg gtgcgtcggt cgaggacctc cgtcagaccg cgctgctcgc
     9541 catgatccgc gccgtcgacc gcttcgaccc gtcgcggggc gtgaccttcc ggaccttcgc
     9601 cgcccgcacg atcgagggcg agctcaagcg gttcctgcgc gaccggtcat gggccgtgcg
     9661 tccgccgcgt cgcgcccagg agctgaacct catcgtgcgt cggaggaccg acgagctggc
     9721 gcagtcgagt ggccggtccc cgacggtggg tgaggtctcg gcgtctctcg acgtgcccgt
     9781 cgacgacgtg ctggccgcgc tcgaagccgg ccgggcccgc tcggcggtcg gcctgtcggc
     9841 accagcccgg gaccccgtgg atcgacgtcc gatcctcgag cggatcgggg cgcccgaccc
     9901 ggccctcgcc tcggtcgacg accggtgcct cgtgcagagc gcggtggacg gactggacct
     9961 ccgcgagcgg caggtgctgc agctgcggtt cgtcgatgac ctcagccagc ccgagatcgc
    10021 ggccgctgtc ggggtgtcgc agtcctacgt gtcgcggctg ctgcgggccg ggttggacca
    10081 cctgcgcgag aaactcgcct cctgaccccg ttctgtgcca ccgacccgtt ctgttcgttg
    10141 ttccacaacg tcgacgttgt ggaacaacga acagaacggg tcaggagggg ggtgtgccgg
    10201 aacgccgcat ctgctcgaaa ctccgacgca gcaggcgcga cacgtgcatc tgcgacagcc
    10261 cgacccgctc cgcgatctgc tcctggctca ggtgctcgta gaagcggagt cggatgatct
    10321 cccgttcccg gggcccgagc gtcgacagca gccgaccgac ctcgtcgcgg tcctccagca
    10381 ggtcctcggc gggggaggag accgtccgag gggtcgtggt cgcgccctga gggtcgaggt
    10441 cgtccaggct gcccgcgcgc cgggccgccg tcgcgccgag ggcgccgatc acctcgtcga
    10501 cgctgacgtc gaggtgatct gcgagctcgc gcaccgtcgg tgagcgcgac agctgctgct
    10561 cgagggcctc cgatgcccgc cggaccgaca cgtggaggtc cttgaccgat cgcggcaccc
    10621 tcagcgccca ggtctggtcg cggaagtggc gtttgagctc gccgtcgatc gtgcgccccg
    10681 cgaacgtgct gaacgccacc ccgcgggtcg ggtcgaagcg gtcgatcgcc ttgaccaggc
    10741 cgagcagggc gacctggcgg agatcgtcgt cctcggtgtc ggatcggcgg tagcgggtcg
    10801 tgaagtgcag cgccaggccc atgttgtcct cgaccaaggc gttgcgcagc gcccggtcgt
    10861 gggtgcgggc gtactcgacc aggcgggggt cgatcggcgc cggtgcagcc tcgcccgcac
    10921 cggctgcctc gcctgccccg cctacctcgt ccgggctcat cgcgggtcgc cgttcgggac
    10981 ggtcactccg cgctcaggtg cgtgagcatg cggaacgctc cgtcgccgat ctcgtggtgg
    11041 tcgacggtgg cgtcgaggat ccgttgggcc agctggtccg gctcggtggg ggcccactcg
    11101 ccgagacatc tcccgctcac gtggatggtc cgctcctcga tccacatccg gatgaggatc
    11161 gtcccgccct cggctgcctc gacgaggatc ccgacgagct cgttgaccgc gacgcgcagg
    11221 tcctcgaggt cgtcgatcgc cgggtcgagc tcagcggcga ggctggcggc ggcgacccgt
    11281 gccatcgaca cgaatcggaa ctccgcgggg atctccaggc ccacggtccg ctgcggtggt
    11341 ccgctctcgg cggtcgggtg ctcgctcacg tggtggtcac ctcgggtcgt gggcgcggcg
    11401 ggcgcggcgg gcgcacctcg cgccgctccg acgctagccc tgcgacaccc ggggcgtggt
    11461 tgtccgctgc gggagttcgg cgcggtgacg atcgtggcgt ccccgcaccg aggtggcgtg
    11521 ttgagcgtgt tcctctcgtc gcgttagggt tacctaacca ccggccatgt gcgcggaacc
    11581 ggccggcgca cccaggacga cgacggaggt cgccgtgttc gcgaacttcc tgatcgggct
    11641 ccgcgagggg ctggaggcgt cgctggtggt gggcatcctc gtcgcctacc tggtcaagac
    11701 cgaccgtcgg gatcagctcc ccaaggtgtg gctcggcgtc gggatcgcgg tcgtcatctc
    11761 gttcgcgttc ggcgccgcgc tcaccttcgg cccacgcgga ctcaccttcg aggcccagga
    11821 agccatcggc ggcacgctgt cgatcgtcgc ggtcggcttc gtcacctgga tggtgttctg
    11881 gatggccgag caggcccgca acatcaaggg cgagctcgag acgcgggtcg actccaccgc
    11941 cggaggtgtg ggccttgtcg tcctcgcgct gctggccgtc gggcgcgagg ggctggagac
    12001 ggccctgttc ctctgggccg cgacccgggc caccggcgac accctcgccc cgttgctcgg
    12061 ggcgctcctc ggactggtcg tcgcggtggt gctgggagtc ctcgtctacc gcggggccct
    12121 ccggctggac ctgcgcaagt tcttcacctg gaccggggga ttcctggtcg tcgtcgccgc
    12181 cggcgtcgtg tcctacgggg tgcacgacct gcaggaggcg gcgatcctgc ccggcctgca
    12241 caacgtcgcc ttcgacgtgt cgggcaccat cccgcccggc tcgtggtacg ggaccctgct
    12301 caagggcacg gtcaacttca acccccggac cacgtggctg cagctcgtct gctggctgct
    12361 ctacctggtg ccgaccatga cgctgttcat catccgggtc cgccgcccct ccgggccggt
    12421 cacgtccacc ctggtggccg cgaccccgga tcagacccat ccgtccccga ccatcacaga
    12481 aggacaccct gcatgacccc caccgtccga cgcctgctcg ccccgtcgct catcgcggtc
    12541 gtggccctgg ccggcgcctg caccgacaac accaaggagg cggcgtcggg atccgacgac
    12601 ggcgggggca agggcccgac gacgatcgag gtcaccgaca ccggttcgga gtgcacgctg
    12661 tcggccaccg aggccccctc gggcaacatc gtgttctcgg tgaccaacaa gggtagcgag
    12721 atcaccgagt tctacctgct ggccgaggac ggaaagtcga tcatcggcga ggtcgagaac
    12781 atcggaccgg gcctcagccg cgacctggtg ctgcgggccg aaccgggctc ctacgtcacc
    12841 gcctgcgtgc ccggcatgac cggcgacggc caccgcggtg acttcaccgt caccgactcc
    12901 ggcgagaccg acgccacctc cggcgagatg aaggcgctcg aggccgccgc caccaagcag
    12961 tacaaggact acgtcaccca gcaggtcggc ttcctggtgg ccgggacgca gaagttcgcg
    13021 gacgcctaca aggccggcga cgacgaccag gcccgggcgc tgtacgcccc gacccgcgcc
    13081 tactgggaga gcatcgaacc cgtggccgag agcttcggcg acctcgaccc gcggctcgac
    13141 ctgcgcgaag ccgacctgga ggcgggccag gagtggaccg gctggcacca gatcgagaag
    13201 cagctctggc cgccggcgga cttcaccgag atcgacaccc ccgaggagcg ggcgaagatg
    13261 gccgaccagc tcgtcgccga cacccaggac ctcgccaccc gcgtcgagac cgtcaccttc
    13321 aaggccgacc agctcggcaa cggcgccaag gagctgctcg acgaggtcgc cacaggcaag
    13381 gtcacaggcg aggaggagtt ctggagccac accgacctgt gggacttcca ggccaacgtc
    13441 gacggcgcac ggctggccta ccaggccctg cgtccgatcg tgctgatcaa ggacaaggac
    13501 ctggccgcca cgctcgacga ggagttcgcg tcgctgcaga ccgagctcga caagtacaag
    13561 accgccgacg gcttccagta ctacacccag ctgacccccg aacaggtgaa ggagctgtcg
    13621 gactcggtca acgcgctggg cgagcccctg agccagctca ccgccgtggt ggtgctctga
    13681 ccgagctgat ctgacgcgcc agcgggccgt ccgggcggcg gcccggcccg aggaggaacc
    13741 atggacgacg acacgaccgg cgagaccgtc gagcgggacg gatcgaccgc ggacctggcc
    13801 accgcatccg gcgacggcgc gggcgcggac gagccggacc ggtccggggt gtcgcgacga
    13861 tggctgctcg gcgccggcgc cgtcggtgcc gcggcggtcg gcgtggcggc cggggccacg
    13921 atcgcctcgg ccagcgagcc cgactcggac ccgtccgagg gcgcggccgc cgcagcggtg
    13981 ccgttccgcg gtgagcacca ggccggcatc gtcaccccgg cccaggaccg actgcacttc
    14041 gcggccttcg acgtgatcac cgacgaccgc gagaagctcg tcgagctcct gcagaagtgg
    14101 agcgcggcag ccgagcggat gaccgagggt cactccgccg gaaccaccgg ggccatgaac
    14161 ggaccgctcg acgcaccgcc ggccgacacc ggcgaggcct acggtctgcc gccgtcgcag
    14221 ctgacgatca ccatcggctt cggtccgacc ctgttcacca aggacggaca ggaccgcttc
    14281 gggctggccg aacgtcgccc cgaggcgttg atcgacctcc cccacttccc ggccgaccgc
    14341 ctggacccgg cacgcagcgg cggcgacctg tgcgtgcagg cctgcgccca cgatccgcag
    14401 gtggcggtgc acgcggtgcg caacctggcc cgcatcggct tcggggtcgt ctcggtccgc
    14461 tggtcacagc tcggtttcgg tcggacgtcg tcgacatcca ccgcacagga cacgcctcgg
    14521 aacctgttcg ggttcaagga cgggaccgcg aacctcaagg ccgaggagcc ggacctgctc
    14581 gacgagttca tctgggtcgg caaggacgac gatcccgccg cggcctggat ggccggcggt
    14641 tcctacctgg tctcgcgtcg gatcagcatg cacatcgaga cctgggatcg cacctcgatg
    14701 cgcgagcagg agatgatctt cggccgggac aagggcgagg gtgctccgct cagcgggggg
    14761 accgagttca ccgagcccga cttcgacgcc gagggtcccg acggtgagcc gaagatcgcc
    14821 ctgatgtcgc acgtccggct ggcgcacccc gacgacaacg acggcgtgca gatcctgcgg
    14881 cggggctaca acttcaccga cggcaccgac ggactcggtc ggctggacgc ggggctcttc
    14941 ttcatcgcgt tcgtccgcga tccccgcacc cagtacgtgc cgatgcagac caagctctca
    15001 cgcgacgacc tgctgatgga gtacctggtc cacaccggct cgggtctctt cgccgtgccg
    15061 ccgggggtca ccggcccggg acgctgggtc ggccaggacc tgttcgcctg atccccccct
    15121 ctctccgttc tgtagtgcga gatccaggtg aacacctgga tctcgcacta cagaacgggt
    15181 ggtggtgacg gctaccctcg cccgacgcac agtccggagc ccgtcggcgg gcccggaacc
    15241 ggagacaggg ggaaccagcg agaatgaccg aggtcgccat ctcgaccgat ccgcggggtc
    15301 ggatcttcat cacgcccgag gcgttcgccg acatggacgc ctggcacgcc accgcagccg
    15361 agatccgccg cgacgagccg gtcatgcgcg tcgaggccga gggctggacc ccgttctggg
    15421 ccatcaccaa gcacgcagac gtgttcgagg tgtcgcgtcg cagcgacgtc ttcttcaaca
    15481 ccgagaagtc ggcgccgggc ccggacctcc agtacgacat gctccaggcc ttcggcatcg
    15541 agctgccccg caccctggtg cacatccacg gcgacgtgca caacaagctg cgctcggtca
    15601 ccaacgactg gttcaagccc gccgcggtcc gtcacctcca accgaccatc gagtccatcg
    15661 cggacgagtt cgtcgacaag atggccgcga tggacgggcg ctgcgacttc gccgcggacg
    15721 tcgcccagcc cttcacgatc cgggtgatca tgagcatctt cggggttccc cagagcgacg
    15781 agcccctcat gctgtcgctc acccaggggc tcttcggcgc cgcggatccc gagtacatgg
    15841 gcgacttctc ggacccgttg gcggccatca cctcgtcgat caaccgcttc gaggagtact
    15901 tcgacgccct cagcgacgag cggcgcgaga acccgaccga cgacctggcc accgtgatcg
    15961 ccaacgggat ggtcgacggg gcaccgatgg gggatcgtga gcggctctgg tactacatca
    16021 tcgtcgcgac cgcaggacac gacaccacgt cgttcgccct ctccggcggc ctcgagcggc
    16081 tgctcgccca ccccgaccag ctgcgggcgg tccaggacga cccgacgctc gccggggcgg
    16141 cggccgagga gatgatccgc tggaccacgc cggtgcgcag cttcatgcgc tgggcccagg
    16201 ccgacaccga gatcggcggc gtgccgatcg cggcggggga cgcggtgctg acctcgtacc
    16261 cgtcggccaa ccgtgacgag gacgtgttcg tcgacccgat gcgcttcgac atcggtcgcc
    16321 ccgacgcgga ccagctcctg agcttcggcc tgggcatgca ctactgcctg gggtcccagg
    16381 tcgcgcgacg tgaggtccgg acgctgctgg ccaaggtgct cgagcgggtc gacaccatcg
    16441 agctcgacgg cgagcccgcg tggtccggtg cccacttcgt cagcggggtc aagcacctgc
    16501 cgatccgcta cacgctgcgc tgagcccggc ggcgagccgg accgcaggac aggatcagac
    16561 gtgcctgaag ggaccacggc ccgagccgcc gggagcgccg gcttccccga cgggttcatg
    16621 tgggggaccg gtgcgtcgtc gctgcagacc gagggagcgg cgccgcgcag cgactggtac
    16681 gcgtgggagg cagccggccg cgccccccga tcgggggacg gcaacgggtt cggggtgcgc
    16741 taccaggagg acttcgccca gctcgccgag ctcggcctga cccaccatcg gctgtcgctc
    16801 gactgggccc ggctcgaacc gcatcccggt cgccacgacc ctgcggcggt cgagcactac
    16861 cgagcggtcc tgaccgcggc ccgtgacgcc ggcatctcgg tgtgggtctg cctgcaccac
    16921 ttcgacctgc ccggctggtt cagcgaggac cggggcggct tcctcgaacc ggaagctcgg
    16981 tcccgcacgt ggccccgaca cgtcgactgg gtcggggaga ccttcggcga cctggtccac
    17041 ggctggatgc cggtcaacga accggtggcc tacgcgatgc tcgggtggct cctcggcacg
    17101 atcccgccgg gacgccggga cccggagcgg ttctcggaag cactcgaggc agtgctgctg
    17161 gccgaacacg aggcgtggag ggtcctgcgc tcgggggacc agcccacctg cacgatccac
    17221 agcctcatgc cggtgttcgc ggcgactgcc ggcgaggacg agcggcggac cgcggcggcg
    17281 cgggccaacg ccgagttggt cgacgaggtg gtctggcgga cctggatccg ggcgatgcgc
    17341 gacggggtgc tccacgtccc gggccgtggc ctgatcgagg tccccgagat ggccggagcg
    17401 ttcgacctga tcggcttcag ctactaccac gcgctcgggg tcacggcgga caacaacttc
    17461 gtcccgcatc cggcggggat ccgtcccggg ccgcacggtc tcccaccgtg ggccgagggg
    17521 cttcgcatct gcctggagcg gttggccgac gatctccccg gccggccgct cgtggtctcc
    17581 gagtgcggcc tgggcacctg gaccgcagag gacgacgacg agcagcggtg ccagtacctg
    17641 accgactgcc tggagatcac ccgtgacgcg gtcgccgacg ggatcgacgt ccggggcttc
    17701 ttccactgga ccggcgtgga caactacgag tggggtctgg ggttcgatgc ggcgttcggt
    17761 ctgatcgacc gggaccgcag ccccaagccg tcggcggagc tggcacggcg atgggcgacc
    17821 ggccgatgaa cggagaccgt tcgatgatca ggcctcgacc tcgcgtccat cggcgtgtcg
    17881 gtccgaggac ggcgccgaca gctccagtga gcccgcgtcg tcggcggtga ccacgacctc
    17941 ctgacccagg tggaacgacg cggccgtcgc cgtgtcgaca cggccgagca cggccgggtc
    18001 gcgcacctgg aggcgcaggc cgtgtcggtc gactccgacg accaccgcgt cgagcggttg
    18061 accgacatga ccggacagga tgacctgttc gaccaggtcc cgggcgccgg agtcggcctg
    18121 cgattcgcgg cgccgcgccg atgccatgag ctcgggcagc acagcaagcg cggcgcggat
    18181 gtcgtcggcg acttcggctc cggcgcagca ggccagcacg acctcgttgg cgaaccggtc
    18241 gacgaggcgt cgcagcggtg ccgtgacgtg ggcgtagggg gcggccagcg cggagtgctc
    18301 ggcgtcggag ccctcgggtg gggcgatacc cgcccgtcgg tcgaacgccg cgtagcccga
    18361 cccgcggagc agtcgggccg cctgggtcgc gaacgcagcc tggtcgggga acgccggatc
    18421 caacgacctg atcacatccg cgtagctgtc ctccggctcc cagcagacac cgagtgcggc
    18481 ggcatcggcc cgcaattcgg ccacggtccg ctcatcgggc ggtggcaggg tccgcagcac
    18541 ccccacgccg gcgtccaaca tgatccgcgc cgcggccatg ccggtcatca gggagatctg
    18601 cgcgttccag tcctcgacgg gaaggggagc ccggaaggtc agaccgatgt gcccgtcggc
    18661 gtcgatgacc acctcctggt ccgggagcgg caacgacacc gcgccccgct cggcggcccg
    18721 gtcctgccgg agccgtccca cctcggccag caacgtcgcg acctccggcc gggtcgcggg
    18781 atcgccgtcg tcgagcgacc gctgcaggga cgggtagtcg agctcggccc gggagcgcac
    18841 gacggcacgc tcgagcacga catcgtcggc cgacccgtcg gcgtcgagat cgagctgcca
    18901 caccagcgcc ggtcggtcct cacccgctcg cagcgacgcg gcgtgctcgc tcagcaccgg
    18961 tgggtgcagc gggatccgac cgtcgggcag gtagatcgtg acgccccgtg cccggcactc
    19021 gtcgtcgagc gggccaccga cgccgacgaa ggcagccacg tccgcgatgg cgtagcggac
    19081 ccggaacccc tgaccgcgac gttcgatgtg gaccgcctgg tccaggtcgg tgctgcctgg
    19141 cggatcgatc gtcacgaagt cgatcccggt caggtcccgg cgccggcgac cggctgcggt
    19201 gtcaccggcg agtccaccgg cgcgggcggc caggtctgcc gccgcgagca cctcgggcgg
    19261 gaactcccgg gggagcgcga gctcctcgcg cagctccctc aggccccgtc ggatccgctc
    19321 gtccggactc gcttcgtccc gcacaggcgc ggtgatcacc gcgcctgtgc gggacaaagc
    19381 gagggagcgg gacacagcga gggtgcggga caacgcgggg gagcgggccg gtcagcggtg
    19441 ctgcagcgag gtccacgcct cggtcagcac cgatcgcagc accgactcga tcagatcgag
    19501 ctcgtcgtcg ccggcgatca acggcggtga cagcaggatc atcggatcgg agcggtcgtc
    19561 ggagcggcac atcagtcccg actcgaacag ccggtgcgag atctggccct tgagcagcca
    19621 ggcgcattcc tcgtcggtga aggtctcgag cgtgtcgggg tcccggacca gctcgacggt
    19681 gcgcaggaac ccgtcgcctc ggacgtcgcc cacgatcggc aggtcgagca ggccctcgag
    19741 tcgctcctgg aagcccgacg ctctccggct cacgttgccg agcaggtcct cgtgctccat
    19801 caggtccagg ttggccatgg cgaccgcgca gctgaccggg tggccgccga aggtgatgcc
    19861 gtgcaggaag gtcgagtgcg cctcgacgaa cggctgggcc agacggtcgc tgacgagcac
    19921 cccgccgagc ggcgagtagc ccgacgtcac gcccttggcg aaggtgatca tgtcgggccg
    19981 gtacccgaag cgatccgatc cgaaccactc gccgaggcgc ccgaacgcgc agatcacctc
    20041 gtcggacacc agcaggacgc cgtggcggtc gcagatctcg cggacgcggg cgaagtaccc
    20101 cggcggcgga ggcagcgtgc cgccggtgtt ctgcaccggc tccaggtaga ccgcggcgac
    20161 cgtgtcgggg ccctccatga ggatccggtc ctcgatgtcg cgagcgcaga tctcgcccca
    20221 ctcctcgtcg aagtcgtgtc ctgatcggaa ggagctggtg tggcggacct tgaccgcgcc
    20281 ggggaccagc ggctcgaacg gctccttgat ggctggcagg ccggtgatcg acagcgctcc
    20341 catcgtggtg ccgtggtagg cgaggtcgcg cgagatcacc ttggtgcgat cgggctggcc
    20401 gaccgccttg aagtactgcc gagcgagctt ccaggcggtg tccaccgcct cgctcccgcc
    20461 ggtggtgaag aacacacggt cgaggtcgcc cggcgccatc ccggtcagcc gctcggccag
    20521 cgccagggcg ggctcggtgg cgtaggacca gatcgggaag tacgccaacg tcgacatctg
    20581 ctccgacgcg gcggcggcca gctcccggcg gccgtggccc acctgcaccg tgaacaagcc
    20641 ggacaggccg tccaggtacc ggttcccggc gcggtcccag acgtagcagc cctcaccgcg
    20701 gaccatcacg ggcaacggat cgtcgcccat ggcggtgaaa tgtccccaga agggcgaggt
    20761 tgcggcggtg gggcccgccg ccggggcggt cgaggcggtc gaagtggtgg ggacgggcga
    20821 tgacacgggt atcggtcctg gttcggcgac gcccccgacg ctacgccgct gctgtgggat
    20881 cggcgagcga tcccgctccc ggccgcggtc cgggtcgatc gaccccgtta gcgtgaccga
    20941 cgtggatcac cgctacgacg acttcccgca cctgcagttc gatcgcccct ccgacggcgt
    21001 cctgcgcatc accctcgacg gccccggact caacgcggtc gacgccgatg tccaccgcga
    21061 gctcgcggac gtctggctcg cggtggaccg cgacccagac acccgggtcg cgctgctgcg
    21121 cggcgccggt cgggcgttct cggcgggggg cagcttcgag ctgctcgacg ggatgatcga
    21181 ggacttcgcc gtgcgcacgc gggtcctgcg agaggcgcgg gacctggtgt tcaacgtgat
    21241 cgactgctcc aagccgatcg tgtcggccct gcacggcccg gcggtcggcg ccggtctggt
    21301 cgccgggctg ctggccgacg tgtcggtcgc ggctcgcacc gcccggatca tcgacggcca
    21361 cacccggttg ggcgtcgccg caggtgacca cgccgcggtg tgctggccgc tgctgtgcgg
    21421 gatggccaag gccaagtact acctgctgac ctgcgacaca ctcagcggcg aggaggccga
    21481 acggatcggc ctggtgtcga tctgcgtcga cgacgaccag gtccaggacc gggcgctggc
    21541 ggtggccgaa cagctggcgg cagggtcggc gtccgcgatc cgctggacca agcacgcgct
    21601 caacaactgg taccgcgatc actcctcgat cttcgacgcc tcgtggggac tcgagttcta
    21661 cgggttcggc ggacccgatg ccgccgaggg cctggcgtcg caccgcgaga agcgggctcc
    21721 ggagttccgc gggccgacca gcgagtgacg gagccggctg cggcgcctca agtcgtggcg
    21781 gacggctcga ccaacacgca gcgggtcccg gtgtagatcg tcggcaggca cttgttgcag
    21841 tgcacgcaca gcccctcgtg gcgcggttcg gactgcatcc ggttcaccag gtcgggttcg
    21901 cgcagcagcg cccgggccat ggcgacgaac tcgaagccct cggccatcgc ctgatcgacg
    21961 gtgtcgcggc ggttgatgcc gccgagcagc acgagcggca tcttcagctc gtcgcggaac
    22021 tgccgcgcga acggcaggaa gaacccctcc tcgaacgggt agtgcgggaa cagcttgggg
    22081 ccgtagaacc gcaggccgaa gccgaccacg gcgggttggc tggcccggaa ctccttgagc
    22141 gggacgtcgc cgcggaagaa gtacatgccg ttgagcagcg agctgccccc ggtgagctgg
    22201 agggcgtcga ggtgcccgtc ggcctcgatc atctgggcga tcggcaggct ctcgtccagc
    22261 catagcccgg cgtcgacgcc gtcggccatg ttgaacttgg cggtgaccgc catgtggtcc
    22321 ggagcggtct cccgcacctt tgccagcact cgtcgtggga aggcgctgcg gttctcgagc
    22381 gagccgccgt agcggtcggt ccggcggttg aggttcgggc tcatgaacga gctgagcagg
    22441 tagttgtggc ccaggtggac ctcgacggag tcgaaccccg agtcggcggc cacccgggtg
    22501 gcggtggcga agtcgtcgac cacgcggtcg agctgttcgg gggtggcgcc gcggacccgg
    22561 gccatggccg gtgcgctgaa ccgggtggag ggggccaggg tgggcatgcc gttggagcgg
    22621 gtgttggcca ccaacccggc gtggccgagc tgagcctgga tcgcggctcc ctcggagtgc
    22681 acggcctcgg tgaggcgagt gaggtcgggc acccgttccg gttccatcac cagggtgtgg
    22741 cgctgcaccc gaccgtcccg ggacacggcg cagtaggcga cggtggtcat cccgacgccg
    22801 ccggcggcga ccctgcggtg gaactcgatc agctcgtcgc tgacggcacc gttgcgcatg
    22861 acgccctcga aggtggcggc cttgatggtg cggttgcgca gggtcagcgg gccgaggcga
    22921 gcgggagcga acgggtcggg tggtggccga tcggacatga ccgaacagta gggcggaata
    22981 cgggatggat ctccttaggc tcggatccgt gccgatccgc gacgaacgtc gactgcccga
    23041 ggagtcctgc ctgcccgccg gccggccgga catcgatcgg ctgggcacgg gggagcttcc
    23101 cgacgaccgg tggtgcgccc tcgtcgccgc cgagctgtcg ccggacctgc tcgtcgtgat
    23161 cgacccgctg ctgcgcgccg tgtggaccaa cgccgcggtc gaacggctcc tcgggctcca
    23221 gcgcggcgag atgctgggcg acccggtcag cgaccacgtc caccccgacg acctgatggt
    23281 ggccgtgggg gcgctgaacg agtcccagcg caacgacggc taccacgtcg ccacccgcat
    23341 ccgcgtccgc aggcgtgacg gctcctacat cgacacccgg gtgacggcga ccaccatcac
    23401 cgacgagtcg ggcacgtgga tggtgctcgc cctgcgcccg gtcgaggacg aggtcgccat
    23461 cgaacgccgc cgcgcccagc tcaaggcgct cgcccagagt gtctacgtgg agtgcgccgc
    23521 ggtccactgg tacgagctgg gtgacaagat ccccgccatc ctcggcgcgc tcgccggcgt
    23581 cgtcggagcg caccgggtcg aggtggtcga accccgcgac ggctccttcg agcgcgtcgc
    23641 gggctggtcg gcgcgaggat ccacatcgtg cgggtccggg cgggtccagc tcctggccga
    23701 ccccgagcgc ctgcgggccg ccccgtgcgt ggtcaccacc gtcgagccgg gtggcaccgg
    23761 ggaccgaccc gactgcgggt gcggcgtgga cgtggagccg acacaccgga tggtggtgga
    23821 gctgtggctc gacgtcgacg gggtcgtccg cctgctcttc gacgagtaca ccgagacgtg
    23881 ggacgacgcc aacgccgaca tcatcgccct gatgtgctcg acgatgctgg ccaccatgcg
    23941 acgatgcgag caggagtccg agctcaacat ccgggccacc cgcgaccctc tcacgcgcct
    24001 gctgaaccgc accgccctgc accaacgcct gggtgagatg ctggcgggca tggacccggc
    24061 ccgtccgccg atcgtcctgt tcgccgatct caaccgcttc aagcagctca acgaccgctt
    24121 cgggcaccgc gagggggacg ccgtcctgtg ccgggtggcg gacgccatct catcgcaggt
    24181 ccgctccgac gacgtcgccg cccggatggg tggcgacgag ttcgtgatcg tggtcgcggg
    24241 cgacgacgtg ccggtggcga cgatggtgtc gagggtgcgt gccagcatcg acgccgcgct
    24301 ggtcgactgg cccgatgtgg gcgtggccgt cggggcgatc gtggccgagg tgggcgagag
    24361 ccccgaggac ctcctcgacc gcgccgatcg ggccatgtac gacgacaagg cacgatcccg
    24421 gagcgcgcgt tcggtcgccc ggacctgagc agccgtcctc gccgtcggct gcccgggagg
    24481 ctgtccggac acggttccgg acgtggttcc gggcggcggt ccggacgctg tccggacgat
    24541 cgcggagcga tccgccgggg ttctcagtga acgccgtgca gctgcagcag ctcttggagc
    24601 tgcgcgtcgc cgatcggcga accgtcgagg tggccgtcgg tgtgacgctc gaggacgatc
    24661 atgccggtgg cgttctcctc gggccaatgg ctccaggtca ggtcgcggcc gtcgggtagc
    24721 gggtagaagc ggttggtggg ggtgatcggc acgtcctcca cggtacggaa cgactcggcg
    24781 ccctccatcg gctgaacgga tcagatcctg ggaggccgga ccgggtacag cgacccagcg
    24841 cgaccgggcc aggatgtcca ggtggacctg ccccccggtg ccgagccgga cccgaccgag
    24901 gcgaccggcc cgtcccgctt cggcgggttc cgggccgggt tcgtggccgt ggcgcccctg
    24961 ctggtcggcg tcgccccgtt cggactcgtc gccggcgcca ccccggtggc ctacgggctc
    25021 ggcgccgacg ccgcggtcgg gttctccgtg atcatgttcg ccggcgcgtc gcagctcgcg
    25081 tcgatcgagg tcctgggcgc ggggggctcg gtcctggccg cggtgctcac cgcctgcacg
    25141 atcaacctcc ggctcatcct ctactccgcc tcgtgctccc cctaccttcg ccgcctcgct
    25201 ccggcacgac ggatggtcat ggcctacctg ctcaccgacg aggcctacgc cctgtcgatc
    25261 gtccgctggt ccgagcaggg ctccgaggcc caggacccgc ccacgctgag gggtcggtgg
    25321 accttctaca tgggggccgc cgcgtcgatg tgggcgacct ggcaggtcag caccctggtc
    25381 ggcatcctcg tcgggaacgc cctcccgtcg ggggttcggc tggacgtcat cgcgccgctc
    25441 gtcttcctgg tgttgctggt gccgaccctg gtcacccgtg cggccctggt cgccgccgct
    25501 gtcggcgggg tgggagcggt cgtggccgcc gagtgcggcg ccgggccgat ggccgtcgtg
    25561 gtcggctcgg tggcggggat cctcgccgga gccgccctcg acgacggtgc tgacgacggc
    25621 gcgggcggag gcgctggtgg cggtgcggat ggtgacgacg gtcgcggaca aggcgggccg
    25681 acgccggcgg agcccctccc gtgagcgccg cctggtggac gatcctgatc ggcgcggcgg
    25741 ccacgttcgc catgcgggcg tcgttcttgg tggtggctcc ccggatggcg cggttcccgc
    25801 cgtgggcgca ccggctcctg cgtcagatcc ctccggcggc gttggcggcg ttggtgctgc
    25861 cggcgttcgt ccggcccgac gggcagctgg agctgtgggg accccgggcg gtcgccggtc
    25921 ttgtcgccgg catcgtcgcg tggcggaccc gcaacaccct ggccacgctc gtggtcggca
    25981 tggcggcggc ggtggcgttc ggggccgtga tgggctgaac ggcccgccga gtggcccggg
    26041 gggtgccgta gggttcgccg catggtcgac cacgtcagct cagagccccg aacccggtcc
    26101 tggtggggtt ggggatggga ggaccaggcg ctgaccgacg ccgagtgcac cgcactcggc
    26161 gggttggtcc ccggcctgcc cgacaccccg ctgccggttc cgcgggtcac cgatctcgac
    26221 ctgccggcgc cccgtgccgc agtgccgggc gcactcgacg cgatctccga cagctcgccg
    26281 gaggcccgcg ctgcccacgc ccgcggcaag gcctaccgcg acgtgatccg ggccctgcac
    26341 ggcgacgtgg gcgcggctcc cgacgtcgtg ctccgcccgc gcgacgaggc cgacgtcgtc
    26401 gccgtgctcg actgggcctc ggacgcggag gtcgcggtcg tcccgttcgg aggcgggtcc
    26461 tcggtcgtcg gcggcgtgga gtgcccggcc ggttcgcacg ccggtgtggc gaccctcgac
    26521 ctgaccgccc tggaccgcgt gctggagatc gaccggacca gccgggccgc ccgcatccag
    26581 gccggtgtgc tgggacccgc gctcgaggac cagctccgcc cgcacgacct cacccttcgc
    26641 ttcttccccc agagcttcga gttctccagc ctgggcggct ggatcgccac ccgggccggc
    26701 ggtcactacg ccaccctgcg gacccacatc gacgacctcg tcgagtcggt ccgcatggtc
    26761 accccgatcg gcgtcagcga gtcgttccgc ctgcccggtt cgggggccgg tccgtcgccg
    26821 gaccgcctgg tgctcggctc cgagggcacg ctcggcgtcg tcaccgaggc gtggatgcga
    26881 ctgcaggacc gaccccgcca ccgacgctcg acgacggcca ccttccagac ggcagccgac
    26941 gcgttcgcgg cggtgcgcgc ggtcgcccag agcgacctga accccgccaa ctgccgcctg
    27001 ctcgacccgg ccgaggcggc cctgtcgggt gcgggcccgg gtgaccggtt cgtgctggtg
    27061 ctgggcttcg agtcggccgt cctgcccgtc ggggaccgct tcgagatgct ggtcgagctg
    27121 gtccgtgacc acggcggcac ggtggccgcc ccccgggtcg acggcgacgg gtcgggctcc
    27181 gagcgcgacg cggtggccga cacctggcgg tccgcgttcc tgcggatgcc gtaccggcgt
    27241 gacgggatgg cccggatgag cgcgctgacc gagaccttcg agaccgcgac cacgtgggat
    27301 cggatcgagg agctgtacga ggtcgtgcga cacgaggtcg gagcggtgat ccgtgaggtg
    27361 accggggtcg acgggctggt cacgtgccgg ctgacccacg tctaccccga cggtgcggct
    27421 ccctacttca cggtgatcgc cgccggccgc cgaggctcgg agctggtcat gtgggaccag
    27481 gtcaaggcgg ccgcgatgga cgtgctgtcc cgttcccggg cgacggtgac gcaccaccat
    27541 gcggtgggtc gggaccaccg gccgggctac gaccgccagc ggcccgagcc gttcgcggtg
    27601 gcgttgcggg cggccaaggc agcgttggat ccggccggtg tgctcaaccc cggggtcctg
    27661 atcgacccct gagccctgat ccgtcgaccc gcttggccac gcacggtggc cgggatccga
    27721 cctccggccc tacctgctca ccctccgtgg tggccaggtc ggaaaatggt ccgaacggcc
    27781 tacgcggatc ctcatgtcgg cgcgctacgt gccgatcggg atgcatgcgc tcccgagttc
    27841 tgcgacacct cctccccgcc gctgtggcca ccggtgctct cctggtcccg accgcgtggg
    27901 ccgtctc
//
LOCUS       DAOQOD010000017        27667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_559_length_27667_cov_10.677495, whole genome shotgun sequence.
ACCESSION   DAOQOD010000017 DAOQOD010000000
VERSION     DAOQOD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 27667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27667
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_27667_cov_10.677495"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /gene="hemE"
                     /locus_tag="PLZ72_03330"
     CDS             complement(<1..1168)
                     /gene="hemE"
                     /locus_tag="PLZ72_03330"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNA35363.1"
                     /translation="MTGSPAAPGPHDRSAFIRTCRGEHAEHTPVWFMRQAGRSLPEYR
                     SIRGTGTILRAIADPDLATEITLQPVRRYGVDAAILYSDIMTPVHAVGFGVDIAPGVG
                     PVVEHPFRDRSDLDRLRPLDAEADTPYVVETVRNLVRDLPVPLIAFAGAPFTVASYLL
                     EGRPSRTYVATKALMHTDEALWHELMDRLADLAIASLRSQVDNGASAVQLFDSWAGAL
                     SPADYERFALPASSKVLAAAGELGVPRIHFGVTTGELLGLMAAAGAEVVGVDWRTPLD
                     AARTRVTDAMQRHRLANPVLLGGDLHENWVGHVKADYTRPDSAALGVEFCGTSISSRA
                     AAADNFAALLAENPHFVFADNTRKGYGLVDFTPTQLTTTLRVVDVPQYQRRVRSF"
     gene            1449..2759
                     /locus_tag="PLZ72_03335"
     CDS             1449..2759
                     /locus_tag="PLZ72_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35364.1"
                     /translation="MVPSSEELEEGTRSRVPRGQAQERLHDACARLLAGVTLDDLTAF
                     VTVARLTQESGVSSGAIYSAVDGGVGRSAPQQLARDAFLSVSHEDDEMVLDILEIIHS
                     SIEAGADRTATFIETIAALATDPVVESARSPERGDYTHFWLAAAVALNDPEVADRVGA
                     LLRGTVSSYERVIGRVLELSGRVLVDGIDRSTLAQMLTAAGDGAAIVLRLDPMAEPDL
                     VRRMFLAVFIAVTRSVDDSDDLFATRLVGGRNPLSLSLSQLEVVRDTVLAIFEREGWA
                     AVTLARVGGLAGLSESDLVSVYPTRHHLAAIAWDEVMAAIGRRSAGRSGRAAVDVLGD
                     LVADLAEAACSRRSLVASLLAARLHDSTRADLSDPEPVDDPAVDLLASVIGDDGGLRR
                     VAARIAVDALLMGAAVSESTPEEITAVMTTGLGALVDSPAGDGR"
     gene            2781..4169
                     /locus_tag="PLZ72_03340"
     CDS             2781..4169
                     /locus_tag="PLZ72_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35365.1"
                     /translation="MADDELAAPTGSTSHSSHTRRATTEERLVEAARSLLAASTLDDL
                     TSFVTVKRLTDDSGLSSGAVYSAFTPDAGVGARSRSAPQVAARRSFWNFGSEAIELVD
                     QLTLLFEQVVDASGDDDLHILQSVADLVSGPVAAQARATTNDGWSYTHLFLGAAVALN
                     DPEVGEFLSDLYAGYERAYQPNLEILMRLSGRVLVDGIDLHQFSEMLISATDACALRL
                     RIDPSTDPAIISRMFLAVWIAMTRHADARDDQLGPRLAVRGQGPLDEGQAEAVRAAVL
                     RVFNRAGWPAVTLAKVAQLAGIPDGRLAGVYPSRHDLAVIVRDELVAGVLRRDDQRTA
                     LAPGARLEALVEDLSDTVTMQRSVAASLLISQLNRVTESGSDTDDEGSLVIIERFAEV
                     IREAAAADGTIDLSGPTVPPAAGDGFRVMARATLELMLLRAAASTMPGRELAALVIDG
                     MEGSGVGVIRSD"
     gene            4268..6346
                     /locus_tag="PLZ72_03345"
     CDS             4268..6346
                     /locus_tag="PLZ72_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA35366.1"
                     /translation="MANHPELEAEQAYVDHAYDCLEQTKRAVEAMRESVEGGPGGTFQ
                     HRYERDVVHDRVEARLQHLELGDLSLVFGRIDLDPAVDDTPEDNDRFYIGRVAVADAR
                     RDPVVVDWRAPVAESFYRATGRDPMGLLRRRHFASRGRTVLGIDDELFGSATDALDRG
                     EVQGYGALISALEESRSGRLSDIVATIQVEQDVIIRSELPGVLVVQGGPGTGKTVVAL
                     HRAAYLLYSHRFPLEGQGVLVVGPNRVFLSYIEQVLPSLGEAGVQIATLGDLVPHVRI
                     GGHDDEDTARVKGDLRMIDVVRHAVRDRQRPLRRALRIPYGVQWLTLSVDRSAEIVHA
                     ARRRFRTHNAGRKLVEQLFWEALAESSRAGDSAEVVKERLRGELAVREAFERMWPVLT
                     PAELLNDLYGSAGLLELAAHRFLEPNEWKLLSRAREPVAAEVVFTHHDVPVLDEALEL
                     LGPRPRHRDDDVVRTYGHIVVDEAQDLSPMQLRVLDRRSLNGSMTIVGDIAQSTGAWA
                     HDDWDQVLEHLPSRRPPRRAELTVGYRVPGPIMDLAARVLRVAAPQLQPPTSIRHTGE
                     EPIVTACTSEDLNAAIAAAVRRELDAVGSGNVAVVVPGSLVERADVALTDAGIDHGRA
                     TRQGLDRQVTVVPTSLVKGLELDSVIVVEPAAILREEVRGPQSLYVAVTRSTKRLSIL
                     HAEPLPDVLR"
     gene            complement(6455..7132)
                     /locus_tag="PLZ72_03350"
     CDS             complement(6455..7132)
                     /locus_tag="PLZ72_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNA35367.1"
                     /translation="MPTDAKGSRSKEFHPAFEEYCEAIWELREDDIDVIQARIAERLE
                     VSRPAVSEMIRRMEGEGLIDVGTTISLTAAGKTLAERVVRRHRLAERFLTDVLGLSWA
                     DAHTEAGKWEHVISEPVEEALARVLGDPTTCPHGNPIPGADYRAPDATTLSAIAIGAE
                     FTVSRIPEELEFEPGMLAFLEENSLQPGHSGRLTALSPDGTATVEIEGRHVGIGRFAS
                     DRILVTA"
     gene            7207..12087
                     /locus_tag="PLZ72_03355"
     CDS             7207..12087
                     /locus_tag="PLZ72_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA35368.1"
                     /translation="MTGTPTNSGADVSGPDHLPGLVGGDPLAAFSEPVRAWFSASFPH
                     PTDAQAAAWPAIAAGEHTLLCAPTGSGKTLAAFLWALDDLGRDPDPEPGVRVLYVSPL
                     RALAIDVDKNLRGPRRGISLAAQRLGTPFREPTIGIRTGDSTESERRRLVRDPPDILI
                     TTPESLYLMLTSQARETLIGVQRVIVDEIHALAATKRGAHLALTLERLEHLVSARGRA
                     PEAVRGSVLQRIGLSATQRPLEVVAGYLGGNLVERTADPTTAPTTAPATVRPDPVERP
                     VRIVDAGVRKELDLQVVVPVEDMGAPAGPVPAPATGAAGSGPTPARGSIWPSIHPRLL
                     ELVEQHRSTLVFVNARRTAERLASRLNELAADTGDGEGAGDGDDGGGGGGGGGGGGGG
                     PRFTGAEAVGLSAADSGHELVKAHHGSLSRERRLQIEDELKRGELRGLVATSSLELGI
                     DMGAVDLVVQVSSPGSVAAGLQRIGRAGHQVGAPSRGRIFPKHRADLLETAVVVKRMT
                     DGLIETTSVPSNPLDVLAQQIVAACALDEWSADDLFDLCRGAANYGGLSREVFEATLD
                     MLAGRYPSEEFAELRPRVVWDRQAGTVRGRSGAQRLAVTSGGTIPDRGLYGVFLPDGT
                     RVGELDEEMVHESRAGETFLLGASTWRIEEITFERVVVTPAPGQPGRMPFWHGDGPGR
                     PAELGRALGAFVRDVRGRPPADALRLLSSEHALDANAARNVLAYLDEQAEATGVVPDD
                     RTIVVERFRDEIGDWRLCIHSPYGAAVHAPWAMVIRGRLEQHVAAVDPGPDGVGAGSD
                     GPAIEVLWSDDGIVVRLPDAADDVPLDLLLGDPDELEAEVVAALPATSVFAARFREAA
                     ARALLLPRRRPDRRTPLWQQRQRAADLLSVAARYPGFPILLEATRECCNDVFDLPALR
                     RVLTEVRSRRIRVVPVQTATASPFARSLLFGWIAVYMYEGDAPLAERRAAALALDREL
                     LRDLLGTEELRSLLDAEVVAQVELELQRLTPERAARDADEVHDLLRVLGPLTPAEVAE
                     RVRPDVRDQLDGWLDDLVRTHRAIRVGADRPWVAAAEDAARVRDGFGLAVPIGLPAAF
                     TDPVDTPVRDLLERHARTHGPFTTTQVASRFALAPEVVRAVLGELLGAGRIAEGDFTP
                     GGTGREWCDVDVLRQLRRRSLAALRNEIAPVESPALARFLPRWQQVGSRHRGPDAVAE
                     VVSMLQGAALPASVLEPDVLAVRVPDYSPADLDAMFASGELLWSGAGALGVHDGRIRL
                     MWRDQAPALLSPPTDPVDSGTDEPHHHVLRDHLRARGASFWADLVAAVAAAELPYDDE
                     TVLAALWDLVWAGEVTNDTLAALRAKVGGSSSRRASGARSRRAAPNLRRVSRLGPPAG
                     AGRWSCTDSWREPVPSDTESATIRALHLLDRYGVLTREMALAEGTAGGFAGVYPVLKL
                     LEERGQVRRGYFVAGLGAAQFATPGAVDRLRVAGAEPVAARLDGVDDDGTDIGARTPW
                     VPDPDRWEAEQQPEVPDAWVLAAVDPAQPFGAALAWPESTGHPARAVGAYVVVVDGEA
                     CAYLEKGGRSLLTFPAAESHPEWVPTLATLVGDGRVRRLRIESVDGASTATSRWVDPL
                     RAAGFVEGYKGLTLGS"
     gene            12236..13339
                     /locus_tag="PLZ72_03360"
     CDS             12236..13339
                     /locus_tag="PLZ72_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35369.1"
                     /translation="MPALRTEITEIVTGLGALGDDLDTVLTADAAPDELRNVDEATWA
                     RLRTAHLDGTHRAEMLAAWNNGRALLRASDGLRNRRPAIVEWKGGHRDPGDESVPADL
                     RIDHVYLISCKYLSKVLHNAAPAAVFDRLLAGGPTRRSDENWFDVVAAAEHQRLYQLV
                     RRAHGPDLELPDGVELPEQVRSLGGPHRDVLRRQLPRRWDRECAAAYQALVDVTAERT
                     AERWRAAVADGPGRDRSLLWRLLRLASAPYFVLGTGPRSSLRLRVDTPWDWNQAFELR
                     RFDIEAQPGGQARVGWRARIRSRADPRIDRGADAPVVEGSIVDRRALGAGEVEVAGHV
                     EIRWSHGRFGGHPEAKVYLDTPHAQVPGYHPLV"
     gene            complement(13347..14966)
                     /locus_tag="PLZ72_03365"
     CDS             complement(13347..14966)
                     /locus_tag="PLZ72_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNA35370.1"
                     /translation="MPDAVRRDPAASVDDGPPAEGAGRRPPNVLVVITDQQRADHVGF
                     AGNPDLRTPNLDALAARSTVFDRAYVANPVCSPNRSSILTGRMPSSHGVIFNDRALPW
                     GAETFVRPLREAGYRTALIGKSHLQLSVDREMVPRIGRDACADGFDNSVYGWEFAERF
                     DGDEDPTPPADYYGFDHVEFATDHGARMAGHHLRWALAKGASRDDVVHPMGPDSPGDR
                     RSPRWWQIYRPPYPAELHCTAFVAERTLAFIDEATSQDRPWMAWMAFPDPHHPLTPPG
                     KWFDRHRAEDMTPPDTFGDPLDDAPPHIRAIRARTDGPLWVMPFGVDDPGLVREALAA
                     TYGMIEFIDAEVGRVLDHLEQIGVADDTIVVFTSDHGEMMGDHSLMLKGNLHYQGVLR
                     VPLTVAAPGFGPARSTSLVSSLDIAPTMLELCGLRGHRGMHGESLRPLLDDPTVSVHD
                     HLLVEDDVSPVIARLIGVPARMRTLMTADARFARDSDGFEQVFDLDSDPAETTDRSDD
                     GWRRDELHTALIDSMMAVDDVGGGISRDRAG"
     gene            complement(15018..15407)
                     /locus_tag="PLZ72_03370"
     CDS             complement(15018..15407)
                     /locus_tag="PLZ72_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNA35371.1"
                     /translation="MSTPPDDLSAEALEFLSERHLATLSTLRADGSPHVVPVGFSYDP
                     TDRRVRIITFSGSQKAANATRGGRAAVSQVDGGRWLTLEGTVEVTDDTERVARAVAGY
                     AARYRTPGERPDRVAIEIAVDRVLGRV"
     gene            complement(15418..18063)
                     /locus_tag="PLZ72_03375"
     CDS             complement(15418..18063)
                     /locus_tag="PLZ72_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNA35372.1"
                     /translation="MLRVALKDLMARKRRLVTTSIAVVLGIAFLTGTQLLSTTLNDSI
                     RSLVADVYQGVDAVVRSPKTTSTPFGQPLRTPVEATVLDEVQGVPGVDRAQGFVEAAG
                     NELIDSKGKVFGGGVGPPTITYNWVEDSIMGTGGVREGRGPTADDEIAIDGTSADTLG
                     LEPGDPVKVATLTGGVKTYTLVGVVGLGADGEGKTGAKPIFFTTAEAQAITGQPGQYN
                     FVAVRAEPGIGQREIARTLADALPQEQVITGRQFVDENQESISKFVDILSTFVSVFGY
                     VALFVAIFIIYNTFSILVQQRTRETALLRAVGARRRQVLGASLLEAVVVGVIASVLGL
                     VLGVIIAMLLVNALQNLFTMAGSPALPGVGNVVFALVVGVGITVVSALAPAWRSTRVP
                     PIAALGEVSIDRSNLSRSRMVVGVLMVLVGGALIGLGLADTGPSPVAMVGVGAVMVLV
                     SFALVIGPTMASPFSRLLARVMGPVGGVSARLAGENAARNPKRTASTAAALTIGVTLV
                     TLIAVIASSIKASTDKMIDQSVTADYVVATVSLTSFGAIPPDLTEKISALDDVAVASP
                     LRFGFMRLLDAKAVANAATATTVPPGTFGVPDDAPPGEDTAVLGMDPRTWFDVVDAGT
                     TDGSPSDLGPGTVAVEKHYAETRGWRLGDTIPVYFGATGRQELEVAMVFEQGLGQTPI
                     WLPMDTFAANQLPLFNSDYQIYVKGADGASMSELRDQLDALVADLPTVAVQDLQQYVE
                     AQTGPINTFLYIVYGLLALAIIIALVGITNTLALSILERTRELGLLRAIGMSRRQLRR
                     TVRYEAAIIAVFGALMGLVLGVVFSAALTVAVAADNPGLVTYHLPVVQLVAITVIAAV
                     AGVVAAWLPARRAARLDVLAAIASN"
     gene            complement(18068..18853)
                     /locus_tag="PLZ72_03380"
     CDS             complement(18068..18853)
                     /locus_tag="PLZ72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35373.1"
                     /translation="MSATSGPSPRSPEPGPPAATQVAAAGAIDAHKVYGEGDTEVRAL
                     DGVTVSFERSRFTAIMGPSGSGKSTLMHCLAGLDRLSSGRVFIGETYLDDLSEGQLTE
                     LRRDKIGFVFQAFNLIPTLSAAENITLPMDLAGRDPDTTWMNQVIDTVGLRSRLSHRP
                     SELSGGQQQRVAVARALASRPEIVFADEPTGNLDSRSGGEILQFMRHAVTDLHQTIVM
                     VTHDAGAAAHADRVVFLADGRIVTEMSDPTAAGVLDLMKQLGD"
     gene            complement(18910..19845)
                     /locus_tag="PLZ72_03385"
     CDS             complement(18910..19845)
                     /locus_tag="PLZ72_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA35374.1"
                     /translation="MRHPRFSVLLPVRDRAEVVGRTVAGVLAQTFGDLELIVVDLGST
                     DRTLDAVRAVADDRVVIFEPDHDVTATAEPSYRDALGMARGRWATVINADVDVDAAWL
                     ARIGRLADGSGAGFVSCGGRQIDPRGTISEFRPVVDGGGPTACLRAGAFATTTDRLRS
                     AADLIGDELDAGRGHAAEVDPAGHFGRVALDLAVLDGAVVLHSPELLLSWHDYCPDPS
                     TAHRTDDEMQFHLALQGIDAQARTPIPDERLLARYATAGAMAAVRLQQGRDARRLFRV
                     ACRARPEVRDHWTRLLAAHLGPLGQRMCGSTVAAE"
     gene            complement(19887..21485)
                     /locus_tag="PLZ72_03390"
     CDS             complement(19887..21485)
                     /locus_tag="PLZ72_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35375.1"
                     /translation="MTTTDEMHDHHDAARRSDRWALPLAALSGTLVLVSNLGGIAVGD
                     DGVGYRATADSLLRGDGLQYFLEHPLTVWPPLWPSLMAGLAKVTPLDPVGAAIALNTA
                     VTVAAVVLVHRLLRRVLDDDHLVLLGTAVVALGSSTIGFGHLLMTDFAFSVVVVAVVL
                     VLLRFRDRRDIWSLVATAALVGLSFFLRYAALYLIPIVALWLLILPPPGAETPRRFGR
                     RVAEAGGFAVLATLAPLAWMLRNHALDGTFTGPRYPSERGPVGNAVDIVATVGKFLLP
                     GVANGRDRLWAVVGVIAVIAAVVLGVRLLVATRLDGESVAARVLGLLGGPTGLLLLLG
                     VGYLTYMLYVRSTTALNRLDLRLLEPAYLPLMACGLLLVDRLGALGPAWPGRGLIVAR
                     VWAGANIAAGIVAVVAFAMGNPYFDGNYSSDTFDRVRANPAVAAVPEGCRVLSNLPNA
                     LYPTMESEWSPRRTGLESSDPVDDLDRLVPTLARTPTCLVWVDEQPRYGHLWTRDQLR
                     RRVELEPLAADGDVSVYRVLPRDP"
     gene            21698..25441
                     /locus_tag="PLZ72_03395"
     CDS             21698..25441
                     /locus_tag="PLZ72_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA35376.1"
                     /translation="MDCPSCGAAGAAGARFCWSCGQPLRAPTEERRVVTVLFADLVGF
                     TALSEHLDPEQVRRLVDHAFERLVRDVTSFGGRVDKIVGDAIVALFGAPVAHEDDAER
                     AVRSGLRMQETLAAMADDDWLPDAGSPIGPDGGPVPALRMRIGINTGEVLVGALRAGG
                     DYTAMGDVVNTASRLQTMAEPGEVLVGGSTHAATASVISYESRGALVPRGRDGAVPVF
                     SAITAVQPPGYRPRARVEAPLVGRGPEMDALTDALGLSVARGRGQIVLIVGEAGVGKT
                     RLADELAHVAAIDHGVAHFNGRCVPYGEANPWWPVAEALRAGCGVHRDDPLTVAHERV
                     TRAVATACRPPDPGSAAPPNELTGDSEEAIVAGLLHLMGYEGPWRGLDGPAVRAEATH
                     ALVSFLEASLRHGPIMVRVSDLHWADQVVLDLIDEVAARLARCPFLLVATARGELQER
                     WSPRAGRFNTLVLNLDPLDRTASAQLLDLLAGAELDPQRRSTLLDRSGGNPFYLEELV
                     TLVGPAPAATVGDPPAGVPVGAVDDGSDRPGATGSASRSGPGVGRGELSGPASDAALP
                     DTLRGLVAARIDGLGRDEQQVLEDAAVWGASGTVEVLQRIATAMRSGEDVTPVVRNLA
                     DQDVLVLDGEEWSFRSDLIREVAYGRLTKRERLIRHLGIAEFLDRKFREADADDGTVD
                     TVTRHFVEAARLDRDLGRAHQERAVVAHAVRWALEAARRAESDASWELARRLYTQALD
                     LLDDESDPVAVESRFRCLVGRSRVRSEVWDEAGARRDAADALELADSLGDPADRAVAL
                     LRLGTAAARAGDSERAEVELSESIRIFEETGDLQGQAEALREKGLAALLRGDSRGAED
                     PIAAALDAFRSVGDRRGEAWCLQNLAWIALSDGRPVAAAEFVAASEEAFREVGDRGGL
                     SWAQGLAAFCRLGEGDLDAAGEIAGRILRESERRGDRFGVGMMLTALAHVELWQGRAR
                     AAAASAERAVTAFASSTELAGTVGLEQAMAIAGRAEIMAGNTGKGRHLLAEAVRSGPG
                     GAPSVYASGVALVTDVQLGRPERVVALDPDLWSVAAGSGRRGDQDAALALALAQSGRV
                     AEAVRRADAAVGTDPDHGYVVACAALVAAAAGDHERARERALRVLGLARATYLDRVWA
                     RIVLALVAPGEGGVDQLDLADGEIGPGEDVVAVALVDLARLVLDARGEDRWRQADGVE
                     PRGLDLGGSEVGRRLWELGLDDTRWADLFVAAVTAGDLAAPAP"
     gene            complement(25506..26138)
                     /locus_tag="PLZ72_03400"
     CDS             complement(25506..26138)
                     /locus_tag="PLZ72_03400"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA35377.1"
                     /translation="MAIRLGDSAPDFSIETTEGTVDFLDWKKGSWAVLFSHPKDYTPV
                     CTTELGTVAKLKDEWAKRNVKVIGLSVDPIEDHRGWAGDIEETQGVPLNFPLIADPDH
                     KVSDLYDMIHPNANDTLTVRSVFIIGDDDKVKLILTYPASTGRNFDEILRVIDSLQLT
                     ANHKVATPANWTEGGEVIISPAIPNDEAKSIYPDGWREEKPYLRYVPQPG"
     gene            26401..26718
                     /locus_tag="PLZ72_03405"
     CDS             26401..26718
                     /locus_tag="PLZ72_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35378.1"
                     /translation="MDNLLDPVTDIQDKVVDGVKRTKQPIADAVATVVGLVLDQVPSV
                     PALPYAELFPTPLEVIDNQAKFATKVVNATKTVATAAAKSAAPLTDQLLDRTAPVSAV
                     KAA"
     gene            complement(26880..>27667)
                     /locus_tag="PLZ72_03410"
     CDS             complement(26880..>27667)
                     /locus_tag="PLZ72_03410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA35379.1"
                     /translation="LFHLWSLSVEEWFYFFWPFLLLLGLRSRARQRTLIAAASAGAGF
                     WVLVRLSGEAVGVNWTHDDPFVGTGVTYVGEVLYRFSAMRFDQLLVGCLLAVVVRRYA
                     LVASEPGAPRRRWLDAAAALGTLVLVAELLLAGRVGAFDPFGSIGFNLALAGIPFAVL
                     WIHLNPRVGPARWLSLPLAVWIGKRAYGLYLWHEVLNILTPKPAGTAALLVRLVVLVV
                     ASMGVAELSWRFIESPFLRRKSARYGDRHQDPTAPAAPSATTD"
BASE COUNT         3769 a         9629 c        10376 g         3893 t
ORIGIN      
        1 cgaatgatcg cacgcgccgc tgatattgcg gcacgtcgac cacgcgcagc gtggtggtga
       61 gctgcgtcgg ggtgaaatcg accaggccat agcccttgcg cgtgttgtcg gcaaaaacga
      121 agtgcgggtt ctcggccagc agcgcggcaa agttgtcggc cgcggccgcg cgcgagctga
      181 tgctggtgcc gcagaactcc acgcccaatg cggcgctgtc gggccgggtg tagtcggcct
      241 tcacgtggcc gacccagttc tcgtgcaggt cgccacccag cagcaccggg ttggccagcc
      301 ggtggcgttg catcgcatcc gtgacgcggg tgcgggccgc gtccagcggc gtgcgccagt
      361 cgacgccgac cacctcggct ccggcggcgg ccatgaggcc caacagctcg ccggtggtca
      421 ccccgaagtg gatgcggggc acgcccagct ccccggcggc ggcgagcacc ttcgagctgg
      481 ccggcagtgc gaaccgctcg tagtccgcgg gcgacagggc gccggcccac gagtcgaaca
      541 gctgcaccgc ggacgcgccg ttgtcgacct gggagcgcag cgaggcgatc gccaggtcgg
      601 ccaggcggtc catcagctcg tgccacagcg cctcgtcggt gtgcatgagc gccttggtcg
      661 ccacgtaggt gcgcgacgga cggccctcga gcaggtagct ggcgacggtg aacggtgccc
      721 cggcgaacgc gatgaggggg acgggcaggt cccggaccag gttgcgcacc gtctcgacca
      781 cgtacggggt gtcggcctcg gcgtcgagcg gtcggagccg gtcgaggtcg gagcggtccc
      841 ggaacgggtg ctccaccacc ggcccgaccc cgggggcgat gtcgacgccg aagccgacgg
      901 cgtgcaccgg ggtcatgatg tcggagtaga ggatggcggc gtcgacgccg tagcgacgga
      961 cgggctggag ggtgatctcg gtcgccaggt ccggatccgc gatcgcccgc aggatcgtgc
     1021 cggtgccacg gatcgagcgg tactcgggca gggaccgccc ggcctggcgc atgaaccaca
     1081 ccggcgtgtg ctcggcgtgc tcaccccgac aggtgcggat gaacgcggac cggtcgtgcg
     1141 gtccgggggc tgcggggctg cctgtcatcg acggtgagtc aaccacggcg ctccggcccg
     1201 gaaaaagcat tcggccagga cgaagtgggg cgtacctcgt cctggccgtg tcgagcctct
     1261 cccttgggcg ggctgacgag agacagtgtc gccgagcgcc gcccctgggg acaggccgaa
     1321 acgggttcac tgaatcatgt tccctctctg gtaacgaacg atctttcgtt acagtggatc
     1381 cgggtcctcg gcacgacacc gtggcgggga acggtcatag ggtgtgcgtg tcgcgaggag
     1441 ggagcgacgt ggtgccgagt tcggaggagc tggaggaggg gacgcgctcc cgggtgccgc
     1501 gcggccaggc gcaggaacgc ctccacgacg cgtgtgcgcg tctgctcgcc ggcgtcaccc
     1561 tcgacgacct caccgcgttc gtgaccgtgg cgcggctgac ccaggaatcg ggggtgtcga
     1621 gcggggcgat ctactcggcg gtcgacgggg gcgtcggccg cagcgcccct cagcagctcg
     1681 cccgcgacgc cttcctgagc gtctcccacg aggacgacga gatggtgctc gacatcctcg
     1741 agatcatcca ctcctccatc gaggccggtg cggaccgaac tgcgacgttc atcgagacga
     1801 tcgccgccct cgccaccgac cccgtggtcg aatccgctcg cagcccggag cgcggcgact
     1861 acacgcactt ctggctggcg gcggcggtgg cgctcaacga cccggaggtc gctgaccggg
     1921 tgggtgcgct gctgcgcggg acggtgagca gctacgagcg ggtgatcggc cgcgtcctgg
     1981 agctgtcggg tcgggtgctg gtcgacggga tcgaccgctc caccctggct cagatgctga
     2041 ccgcggccgg tgacggcgcc gcgatcgtcc tccggctcga ccccatggcc gagccggacc
     2101 tggtccggcg gatgttcctg gcggtcttca tcgccgtgac ccgcagcgtc gacgactccg
     2161 acgacctgtt cgccacccga ctcgtgggcg ggcgtaaccc gctgtcgctg tcgctgtcgc
     2221 agctcgaggt ggtgcgcgac acggtgctgg ccatcttcga gcgcgagggc tgggcggcgg
     2281 tgaccctggc ccgggtcggc ggtctggccg gcctgtccga gtccgacctg gtctcggtgt
     2341 atccgacccg tcaccatctc gccgcgatcg cgtgggacga ggtgatggcg gccatcggcc
     2401 ggcgctcggc ggggcggtcg ggacgggccg cggtcgacgt gctcggcgac ctggtggccg
     2461 acctggccga ggcggcgtgc tcacgtcgct cgctggtggc atcgctgctg gccgcccgtc
     2521 tccacgactc gacgcgtgcc gacctctcgg acccggaacc cgtcgacgac ccggcggtgg
     2581 atctgctggc gagcgtgatc ggcgacgacg gtggtctgcg tcgggtcgca gcgcggatcg
     2641 ccgtggacgc cctcctgatg ggagccgccg tctcggagtc gactcccgag gagatcaccg
     2701 ccgtgatgac caccggactg ggagctctcg tcgactcacc ggccggagac ggccgatgac
     2761 ccacacgagg aggcggcctg atggccgatg acgagctggc cgcacccacg gggtcgacgt
     2821 cgcattcgag ccacacgcgg cgggcgacga ccgaggagcg gttggtcgag gccgcacggt
     2881 cgttgctggc ggcctccacg ctcgacgacc tgacctcctt cgtcacggtc aagcggctga
     2941 ccgacgactc gggcctgtcg agcggcgcgg tgtactccgc cttcacgccc gacgccgggg
     3001 tcggtgcgag gagtcggagc gccccccagg tcgcggcccg ccgatccttc tggaacttcg
     3061 ggtccgaggc gatcgagctc gtcgaccagc tgaccctgct cttcgagcag gtcgtcgacg
     3121 cgtccggcga cgacgacctc cacatcctcc agtcggtggc cgacctggtg tcgggaccgg
     3181 tggcggccca ggcccgggcc accaccaacg acgggtggag ctacacccat ctcttcctgg
     3241 gggcggcggt ggcgttgaac gacccggagg tgggtgagtt cctgagcgac ctgtacgcgg
     3301 gctacgagcg tgcctaccag cccaacctcg agatcctgat gcgcttgtcg ggtcgcgtgc
     3361 tcgtcgacgg gatcgacctc caccagttct ccgagatgct gatcagcgcg accgacgcct
     3421 gtgcgctccg gctccggatc gatccgtcga ccgacccggc gatcatcagc cgcatgttcc
     3481 tggcggtctg gatcgccatg acccgtcacg ccgacgcccg tgacgaccag ctcgggcccc
     3541 gcctggccgt ccggggccag gggcccctcg acgagggtca ggccgaagcg gtccgggcgg
     3601 cggtgctgcg cgtgttcaac cgggcggggt ggccggcggt gaccctggcc aaggtggcgc
     3661 agctcgcggg gatccccgac ggtcgcctgg ccggcgtgta ccccagccgc cacgacctcg
     3721 cggtgatcgt ccgcgacgag ctggtggccg gcgtgctgcg ccgggacgac cagcggaccg
     3781 cgctcgcgcc cggcgcccgt ctggaggcgc tggtcgagga cctgagcgac acggtcacca
     3841 tgcagcgctc ggtggccgcg tctctcctga tctcccagct caaccgcgtc accgagtcgg
     3901 gttcggacac agacgacgaa ggttcgctgg tgatcatcga acggttcgcc gaggtgatcc
     3961 gggaggccgc ggcggcggac gggaccatcg atctcagcgg gccgaccgtt ccccccgcgg
     4021 cgggcgacgg gttccgcgtg atggcccggg cgaccctgga gctcatgctg cttcgcgctg
     4081 cggcctcgac gatgcccggt cgggagctcg ccgcactcgt catcgacggc atggaggggt
     4141 ccggggtggg tgtgatccgg agcgactgac ctggacggcg gtcgccgtgg gccctgttga
     4201 gccgcagggt cggggactac actggctgat tccccgtttg cgggtcgggc ccgcggcagg
     4261 agcaggtgtg gcgaaccatc ccgagctgga agctgagcag gcctacgtcg accacgcgta
     4321 cgactgcctg gagcagacca agcgggcggt cgaggccatg cgggagtccg tcgagggggg
     4381 tcccggcggg accttccagc accgctacga gcgcgacgtc gtccacgatc gggtcgaggc
     4441 ccgcctccag cacctcgagc tcggcgacct gtcgctcgtg ttcggtcgga tcgacctcga
     4501 cccggccgtc gacgacacgc ccgaggacaa cgaccgcttc tacatcggtc gtgtcgccgt
     4561 ggccgacgcc cgtcgggacc cggtggtcgt cgactggcgg gcgccggtgg ccgagtcgtt
     4621 ctaccgggcg accggccgcg acccgatggg tctgctccgg cggcggcact tcgccagtcg
     4681 gggtcgcacc gtgctcggca tcgacgacga gctattcggt tcggccacgg acgccctcga
     4741 ccgaggcgag gtgcagggct acggcgcttt gatctcggct cttgaggagt cacggtcggg
     4801 ccgcctgtcc gacatcgtcg ccaccatcca ggtcgaacag gacgtcatca tccgctcgga
     4861 gctgcccggc gtcctggtcg tccagggcgg tcccggcacc ggcaagacgg tcgtggcgct
     4921 gcaccgggcc gcctacctgc tctacagcca ccgcttcccg ctcgagggtc agggggtcct
     4981 ggtcgtgggc cccaaccgcg tgttcctgtc gtacatcgag caggtgctcc ccagcctggg
     5041 cgaggcgggc gtgcagatcg ccaccctcgg cgacctggtg ccccacgtcc gcatcggcgg
     5101 ccatgacgac gaggacaccg cccgggtcaa gggcgacctg cggatgatcg acgtcgtccg
     5161 ccacgcggtc cgcgaccggc agcgcccgct gcgccgagcc ctgcggatcc cctacggcgt
     5221 gcagtggctg acgttgtcgg tcgaccgttc ggccgagatc gtgcacgccg ctcggcggcg
     5281 gttccgcacc cacaacgccg gccgcaagct ggtcgagcag ctgttctggg aggcgctcgc
     5341 ggagtcctcg cgggccggcg actcggccga ggtggtcaag gagcggctgc gcggcgagct
     5401 cgcggtgcgc gaggcgttcg agcggatgtg gccggtgctg acccccgccg agctcctcaa
     5461 cgacctgtac gggtcggcgg gtctgctcga gctcgcggcc catcgcttcc tcgagccgaa
     5521 cgagtggaag ctcctgtcga gggcccgcga gccggtcgcg gccgaggtcg ttttcaccca
     5581 ccacgacgtg ccggtcctcg acgaggcgct cgagctgctc gggccccgcc cccgccaccg
     5641 tgacgacgac gtggtccgca cctacggcca catcgtcgtc gacgaggccc aggacctgtc
     5701 gccgatgcag ctgcgggtcc tggatcgccg gtcgctcaac ggctccatga cgatcgtcgg
     5761 cgacatcgcc cagtccaccg gggcgtgggc gcacgacgac tgggaccagg tgctcgagca
     5821 cctgccctct cgtcggccgc cgcgccgggc ggagctcacc gtcggctacc gggtgcccgg
     5881 cccgatcatg gacctcgccg cccgggtgct gcgcgtcgcg gcgccgcagc tgcagccgcc
     5941 gacgtcgatc cgccacaccg gcgaggagcc gatcgtcacg gcgtgcacgt ccgaggacct
     6001 caacgccgcg atcgcggcgg cggtgcggcg cgagctcgac gccgtcgggt cgggcaacgt
     6061 ggcggtggtg gtccccggat cgttggtcga gcgggccgac gtcgcgctca ccgacgccgg
     6121 catcgaccac ggacgggcga cccgccaggg cctggaccgt caggtgacgg tggtgccgac
     6181 gtcgctggtg aaggggctgg agctcgactc ggtgatcgtg gtcgagcccg ccgcgatcct
     6241 gcgcgaggag gtgcgggggc cgcagtcgct gtacgtggcg gtcacccggt cgaccaagcg
     6301 gttgtcgatc ctgcacgccg agccgctgcc cgacgtgctg cgctgagcgc ccctcgcttt
     6361 gtcccgcccc tcgctttgtc ccgcacagac gtcgtgatca cgacgtctct gcgggacaaa
     6421 gcggggtgtg gggtggttca gggtgtgggg tggttcaggc ggtgaccagg atgcggtcgg
     6481 acgcgaagcg accgatgccg acgtgtcggc cctcgatctc gaccgtggcg gtcccgtcgg
     6541 gcgacagcgc ggtcagccgg ccggagtggc cgggctgcaa agagttctcc tcgaggaacg
     6601 ccagcatccc cggctcgaac tcgagctcct cggggatgcg gctcacggtg aactcggcgc
     6661 cgatggcgat ggcgctgagg gtggtcgcgt cgggggcccg gtagtcggcg ccggggatcg
     6721 ggttgccgtg cgggcacgtg gtcgggtcgc cgagcacccg ggccagcgcc tcctcgaccg
     6781 gctccgagat gacgtgctcc cacttgccgg cctcggtgtg ggcgtcggcc cacgacaggc
     6841 cgagcacgtc ggtcaggaag cgttcggcca gacggtggcg gcgcaccacc cgctcggcca
     6901 aggtcttgcc cgcggcggtc agcgagatgg tggtgccgac gtcgatcagg ccctcgccct
     6961 ccatgcggcg gatcatctcc gacacagcgg ggcgggacac ctccaacctc tcggcgatcc
     7021 gggcctggat cacgtcgatg tcgtcctcgc gcagctccca gatggcttcg cagtactcct
     7081 cgaacgcggg gtggaactcc ttggagcggc tgcccttggc gtcggtcggc atgggcggga
     7141 tcctacttgc gggtccggtc ccggaccggc cgggtgggtg tcggaggtgc tcggtagcgt
     7201 gcgggcgtga ccgggacgcc caccaactcc ggagcggacg tgtccggccc ggaccatctt
     7261 cccgggttgg tcggcggtga tccgctggcc gcgttctccg agccggtccg cgcctggttc
     7321 tcggcctcgt tccctcaccc gaccgacgcc caggccgcgg cgtggccggc gatcgcggcg
     7381 ggggagcaca ccctgctgtg cgccccgacc gggtcgggca agaccctcgc cgcgttcctg
     7441 tgggcgctcg acgacctggg ccgcgacccc gatccggagc cgggggtccg ggtcctgtac
     7501 gtgtcgccgc tgcgggctct ggccatcgac gtcgacaaga acctgcgggg gccgcggcgc
     7561 ggcatctcgt tggccgcgca acggctcggg acgcccttcc gggagccgac catcgggatc
     7621 cgaaccggcg acagcaccga gtcggaacgc cggaggctgg tgcgcgatcc gcccgacatc
     7681 ctcatcacca ccccggagtc gctgtacctg atgctcacat cgcaggcgcg ggagacgctg
     7741 atcggcgtgc agcgggtcat cgtcgacgag atccacgccc tggcggccac caagcgggga
     7801 gcacacctgg cgctgacgct cgagcggctg gagcacctgg tgtccgcccg gggccgggcg
     7861 cccgaggcgg tgcgcgggtc tgtgctgcag cgcatcgggc tgtcggccac gcaacggccg
     7921 ctggaggtgg tggcgggcta cctgggaggc aacctcgtcg agcggaccgc cgacccgacg
     7981 accgctccga cgaccgctcc ggcgaccgtc cgacccgacc ccgtggagcg accggtccgc
     8041 atcgtcgacg cgggcgtccg caaggagctc gacctgcagg tggtcgtccc ggtcgaggac
     8101 atgggagcgc cggccgggcc ggtccctgct ccggcgacgg gtgcggccgg ttccggaccc
     8161 acgcccgccc gaggttcgat ctggccgtcg atccaccccc gactgctgga gctggtcgag
     8221 cagcaccgct cgacgttggt gttcgtcaac gcccgccgca ccgccgaacg cctggccagc
     8281 cgcctgaacg agctggccgc cgacaccggg gacggcgagg gcgccggtga cggtgacgac
     8341 ggtggcggtg gtggcggtgg tggcggtggt ggcggtggcg gaccccggtt caccggagcg
     8401 gaggcggtcg gtctgtcggc ggccgactcc ggccacgagc tggtcaaggc acaccacggc
     8461 tcgctgtcca gggagcgtcg cctgcagatc gaggacgagc tcaagcgcgg cgagctgcgc
     8521 ggcctggtcg cgaccagctc cctcgagctc gggatcgaca tgggcgccgt cgacctggtg
     8581 gtgcaggtca gctcgccggg cagcgtggcc gccggcctgc agcggatcgg ccgcgccggt
     8641 caccaggtcg gagcgcccag ccgcggtcgc atcttcccca agcaccgcgc cgacctgctc
     8701 gagacggccg tggtcgtgaa gcggatgacc gacggtctga tcgagaccac gtcggtgccg
     8761 tccaacccgc tcgacgtcct cgcccagcag atcgtcgccg cgtgcgccct cgacgagtgg
     8821 tccgccgacg acctgttcga cctgtgtcgc ggagcggcga actacggcgg tctgtcgcgg
     8881 gaggtgttcg aggccaccct cgacatgctc gccggtcgct acccgtccga ggagttcgcc
     8941 gagctgaggc cccgggtggt gtgggaccgc caggccggca ccgtccgggg tcgcagcgga
     9001 gcacagcgcc tggcggtcac ctcgggcggc accatccccg accgcggcct ctacggcgtg
     9061 ttcctgcccg acggcacgcg ggtcggcgag ctggacgagg agatggtcca cgaatcccgg
     9121 gccggagaga cgttcctgct gggcgcgtcg acgtggcgga tcgaggagat caccttcgag
     9181 cgcgtcgtgg tgacaccggc tccgggccaa ccgggacgca tgccgttctg gcacggcgac
     9241 ggtcccgggc gacccgccga gctcggccgg gccctgggcg ccttcgtgcg cgacgtgcgc
     9301 gggcgaccgc ccgccgacgc gcttcgattg ctgagctccg aacacgccct cgacgccaac
     9361 gcggcgcgca acgtcctcgc ctacctcgac gagcaggccg aggccaccgg cgtggtcccc
     9421 gacgaccgga cgatcgtcgt cgagcgattc cgggacgaga tcggcgactg gcggctgtgc
     9481 atccactcgc cctacggggc cgcggtccac gccccgtggg ccatggtgat ccgggggcgt
     9541 ctggagcagc acgtcgccgc cgtcgacccg gggccggacg gcgtcggcgc aggatcggac
     9601 ggtccggcga tcgaggtcct gtggtccgac gacggcatcg tcgtgcgcct gcccgacgcc
     9661 gcggacgacg tgccgctcga cctcctgctc ggcgatcccg acgagctcga ggccgaggtc
     9721 gtggccgccc tgcccgccac cagcgtcttc gccgcccggt tccgcgaggc ggcggcccgg
     9781 gcgctgctgc tgccccgtcg ccgccccgac cgtcgaaccc ccctgtggca gcagcggcag
     9841 cgggccgcgg acctgctgtc ggtggcggct cgctaccccg ggttcccgat cctgctcgag
     9901 gccacccgcg agtgctgcaa cgacgtgttc gacctgccgg cgcttcgccg ggtgctcacc
     9961 gaggtccgca gccgacggat ccgggtggtg ccggtgcaga ccgccaccgc gtcgccgttc
    10021 gcccggtcgc tgctgttcgg gtggatcgcg gtctacatgt acgagggcga cgctcccctc
    10081 gccgagcggc gggccgctgc gctcgccctc gaccgggagc tgctgcgcga cctgctcggc
    10141 accgaggagc tgcggtcgct gctcgacgcc gaggtggtcg cccaggtcga gctcgagctg
    10201 cagcggctga cgcccgagcg ggcggcccgt gacgcggacg aggtccacga cctgctccgg
    10261 gtgctcggcc cgctcacacc ggcggaggtg gccgaacggg tccgccccga tgtccgcgat
    10321 cagctcgacg ggtggctcga cgacctggtc cggacccacc gcgccatccg ggtcggggcc
    10381 gaccggccgt gggtcgcggc ggccgaggac gccgcccggg tgcgcgacgg gttcggcctg
    10441 gccgtcccga tcggtctgcc ggccgcgttc accgacccag tcgacacacc ggtgcgcgac
    10501 ctgctcgaac gccacgcccg cacccacggc ccgttcacca cgacccaggt cgcgtcgcgg
    10561 ttcgcgctgg cgcccgaggt cgtgcgtgcc gtgctcggcg agctgctggg cgccgggcgg
    10621 atcgccgagg gcgacttcac cccgggcgga accgggcgtg agtggtgcga cgtcgacgtc
    10681 ctgcgccaac tgcgtcgacg ttcgctcgcc gctctgcgca acgagatcgc gccggtggag
    10741 tccccggcgc tggcccgttt cctgccgcgg tggcagcagg tcggttcccg ccaccggggt
    10801 cccgacgcgg tggccgaggt cgtgtcgatg ttgcagggcg ccgcgctgcc cgcgtcggtg
    10861 ctggaacccg acgtgctcgc ggtgcgggtt ccggactact cgccggccga cctcgacgcc
    10921 atgttcgcga gcggcgagct gctgtggtcc ggcgccggag cgttgggcgt gcacgacggg
    10981 cggatccgtt tgatgtggcg cgatcaggcc ccggcgctgc tgtcgccgcc gaccgatccg
    11041 gtcgactcgg ggaccgacga gccccaccac cacgtgctgc gcgaccacct ccgggcccgg
    11101 ggggcgtcgt tctgggccga cctggtcgcg gcggtggccg cggcggagct cccctacgac
    11161 gacgagacgg tcctggccgc gctgtgggac ctggtgtggg ccggcgaggt caccaacgac
    11221 acgctcgccg ctctgcgggc caaggtcggt ggctcgtcgt cgcgacgtgc ctcgggggcg
    11281 cggtcccgtc gggccgcgcc gaacctgcgc cgggtcagcc gcctgggacc tccggcgggg
    11341 gcggggcgat ggtcgtgtac cgactcgtgg cgcgagcccg tcccgtcgga caccgagtcg
    11401 gccacgatca gggcgttgca tctgctcgac cgctacgggg tgctcacccg ggagatggcg
    11461 ctggccgagg gcaccgccgg cgggttcgcc ggggtgtacc ccgtgctcaa gctgctcgag
    11521 gagcgcggcc aggtccgacg cggctacttc gtcgccgggc tgggggcggc ccagttcgcc
    11581 actccgggag cggtcgaccg gctgcgcgtc gcgggggccg aaccggtcgc agcccgcctc
    11641 gacggcgtcg acgacgacgg caccgacatc ggggcgcgca ccccctgggt tcccgacccg
    11701 gaccgctggg aggccgagca gcagcccgag gtgcccgacg cctgggtcct ggccgcggtc
    11761 gacccggccc agccgttcgg cgccgcgctg gcctggcccg agtcgaccgg ccatcccgcc
    11821 cgtgcggtcg gggcctacgt cgtcgtcgtc gacggcgagg cctgcgccta cctggagaag
    11881 gggggtcgca gcctgctgac gttcccggcg gccgaatcgc accccgagtg ggttcccacc
    11941 ctggcaacgc tggtggggga cggccgggtc cggcgtctgc gcatcgaatc cgtcgacggc
    12001 gcgtcgaccg ccacctcgcg ttgggtcgat cccctgcggg cggccgggtt cgtcgagggc
    12061 tacaagggcc tcaccctcgg gtcctgaccg cgtccgccgc cggcgatgtt gtagctggcg
    12121 gtcgctgggg tgacctgtgg gctacaagtt cggtgggggg cggcgatgtt gtagctggcg
    12181 gtcgctgggg tgacctgtgg gctacaagtt cggcgggggc tgacaggatg gcccgatgcc
    12241 ggcgttgagg accgagatca ccgagatcgt caccggcctc ggcgcgttgg gcgacgacct
    12301 cgacacggtg ctgaccgcgg acgcggcgcc cgacgagctc cgcaacgtcg acgaggcgac
    12361 atgggcccgc ctgcggaccg ctcacctcga cggcacccac cgggccgaga tgttggcggc
    12421 gtggaacaac ggccgggcgc tccttcgggc ctctgacgga ctgcggaacc gtcggcccgc
    12481 catcgtcgag tggaaaggcg gccaccgcga ccccggcgac gagtcggtgc cggccgatct
    12541 gcgcatcgac cacgtctacc tgatcagctg caagtacctc tccaaagtgc tgcacaacgc
    12601 ggcaccggcc gcggtgttcg accgactgct cgccggaggg ccgacccgcc ggtccgacga
    12661 gaactggttc gacgtcgtgg ccgcggccga gcaccagcgg ctgtaccagc tcgtcaggcg
    12721 cgcccacggc cccgatctgg agctccccga cggcgtcgag ctgcccgaac aggttcgctc
    12781 gctgggcggc ccgcaccgtg acgtcctgcg ccgccagctc ccacgccggt gggaccgaga
    12841 atgcgccgcg gcgtaccagg ccctcgtcga cgtgaccgcg gagcggaccg ccgagcggtg
    12901 gcgggccgcg gtcgccgatg gtccgggccg ggaccgcagc ctgctgtggc ggttgctccg
    12961 actcgcgtcg gcgccgtact tcgtgctcgg caccggaccg cggtcgtcgc tgcggctgcg
    13021 ggtcgacacc ccgtgggact ggaaccaggc cttcgagctg cgccggttcg acatcgaggc
    13081 ccagcccggc ggtcaggccc gggtcggctg gcgggcccgg atccgctcac gggccgaccc
    13141 caggatcgac cgcggggccg acgccccggt cgtcgagggg tcgatcgtcg atcgccgcgc
    13201 gctcggtgcg ggagaggtcg aggtggccgg acacgtcgag atccgatgga gccacggccg
    13261 cttcggaggc caccccgaag ccaaggtgta cctggacacg ccccacgccc aggtccccgg
    13321 ctaccacccg ctcgtctgac ccgggctcag cccgcgcggt cccgcgagat gccgccgccg
    13381 acatcgtcga cggccatcat cgagtcgatc agagcggtgt gcaactcgtc gcggcgccac
    13441 ccgtcgtcgg agcggtcggt ggtctcggcc gggtcgctgt cgaggtcgaa gacctgctcg
    13501 aacccgtcgc tgtcgcgggc gaaccgggca tccgcggtca tcaacgtgcg catccgggcg
    13561 gggacgccga tgagccgggc gatgaccggc gacacgtcgt cctcgacgag cagatgatcg
    13621 tgcaccgaga cggtcgggtc atcgagcaga ggccgcagcg actcgccgtg catgccccgg
    13681 tgcccccgga gcccgcacag ttcgagcatg gtgggcgcga tgtcgaggct cgacaccagc
    13741 gacgtgctcc gggcgggccc gaaccccggc gccgcgaccg tcaacggcac ccgcagcacc
    13801 ccttggtagt gcaggttccc cttcagcatc agggagtggt cgcccatcat ctcgccgtgg
    13861 tcggaggtga acacgacgat cgtgtcgtcc gcgacgccga tctgctcgag gtggtcgagg
    13921 acccggccga cctcggcgtc gatgaactcg atcatcccgt aggtggcggc gagcgcctca
    13981 cggaccagac cggggtcatc gacaccgaac ggcatgaccc agagcggtcc gtcggtgcgg
    14041 gcacggatcg cccggatgtg cggcggggcg tcgtcgaggg ggtcgccgaa cgtgtcgggc
    14101 ggggtcatgt cctcggcccg gtggcgatcg aaccacttcc caggcggggt caacggatgg
    14161 tgggggtcgg ggaaggccat ccacgccatc cacggtcggt cctgcgacgt cgcctcgtcg
    14221 atgaaggcca gcgtgcgctc ggcgacgaac gccgtgcagt gcagctcggc cgggtagggc
    14281 gggcggtaga tctgccacca cctgggggac cggcggtccc cggggctgtc gggccccatc
    14341 gggtggacga cgtcgtcgcg tgacgccccc ttggccaggg cccagcggag gtggtgtccg
    14401 gccattcggg caccgtggtc ggtcgcgaac tcgacgtggt cgaacccgta gtagtccgcc
    14461 ggcggcgtcg gatcctcgtc gccgtcgaat cgctcggcga actcccagcc gtagaccgag
    14521 ttgtcgaacc cgtcggcgca ggcgtcgcgg ccgatccggg ggaccatctc gcggtcgacc
    14581 gagagctgca ggtgggactt gccgatcagc gccgtgcgat agcccgcctc gcggaggggc
    14641 cggacgaagg tctcggctcc ccacggcagc gcacggtcgt tgaagatgac cccgtggctg
    14701 ctcggcatcc ggccggtcag gatcgacgag cggttggggc tgcacaccgg gttggccacg
    14761 tacgcccggt cgaacaccgt cgagcgggcg gccagcgcgt cgaggttcgg ggtgcgcagg
    14821 tcggggttgc cggcgaagcc gacatggtca gctcgctgct ggtcggtgat gaccaccagc
    14881 acgttgggcg gccgtcggcc ggccccctcg gcgggtggtc cgtcgtccac tgaggcagcg
    14941 ggatcgcgtc gcacggcgtc gggcatgaga gtcgttgtag cggacgaccg gtcgggccgg
    15001 ggcccgatcg gttcagctca cacccgaccc aggacccggt cgaccgcgat ctcgatcgcc
    15061 acgcggtcgg ggcgttcccc cggggtccgg taccgggcgg cgtagccggc gaccgcccgg
    15121 gcgacccgtt cggtgtcgtc ggtgacctcg acggtgccct ccagggtgag ccaccgtccg
    15181 ccgtcgacct ggctgaccgc ggcgcgtccg ccccgcgtcg cgttggcggc cttctgggac
    15241 ccggagaagg tgatgatgcg cacccgccga tcggtcgggt cgtaggagaa ccccaccggg
    15301 acgacgtggg gtgagccgtc ggcccgcaga gtggacaggg tggcgaggtg acgctcggag
    15361 aggaactcga gcgcctcggc ggacaggtcg tcgggtgggg tgctcacgac gtcccgtcta
    15421 gttgctggcg atcgcggcca gcacgtcgag gcgagcggcc cgccgggccg gaagccacgc
    15481 cgccaccacg ccggccaccg cggcgatcac cgtgatggcc accagctgca cgaccgggag
    15541 gtggtaggtg accaggcccg ggttgtcggc ggcgacggcc accgtcaacg cagcggagaa
    15601 cacgacaccc agcacgagtc ccatcagtgc gccgaacacg gcgatgatcg ccgcctcgta
    15661 gcggacggta cgtcggagct gccgtcgcga catgccgatg gcccggagca ggccgagctc
    15721 gcgggtcctc tcgaggatcg acagcgccag cgtgttggtg atgccgacca gggcgatgat
    15781 gatggcgagg gcgagcagcc cgtacacgat gtagaggaat gtgttgatcg ggccggtctg
    15841 ggcctcgacg tactgctgga ggtcctgcac cgcgacggtc ggcaggtccg cgaccaacgc
    15901 gtcgagctgg tcgcgcagct ccgacatgga tgcgccgtcg gcgcccttga cgtagatctg
    15961 gtagtcggag ttgaacaacg gcagctggtt cgcagcgaag gtgtccatgg gcagccagat
    16021 cggcgtctgg ccgaggccct gctcgaacac catcgccacc tccagctcct ggcggccggt
    16081 cgcgccgaag tacaccggga tcgtgtcccc cagccgccag ccgcgggtct cggcgtagtg
    16141 cttctcgacc gcgacggttc ccggtccgag gtcggagggg gagccgtcgg tggtgccggc
    16201 gtcgacgacg tcgaaccagg tgcgggggtc cattccgagg acagcggtgt cctcgcccgg
    16261 aggtgcgtcg tccggaacgc cgaaggtgcc gggcggaacc gtggtcgccg tggcggcgtt
    16321 cgccaccgcc ttggcgtcga gcagtcgcat gaagccgaag cgcagcggac tggccaccgc
    16381 cacgtcgtcg agcgcggaga tcttctcggt cagatccgga gggatggccc cgaacgaggt
    16441 gagcgacacg gtggcgacca cgtagtcggc ggtgacggac tggtcgatca tcttgtcggt
    16501 ggacgccttg atcgacgacg cgatcacggc gatgagggtg accagcgtga cgccgatggt
    16561 cagcgccgct gcggtcgacg cggtgcgctt ggggttgcgc gccgcgttct cacctgccag
    16621 tcgggccgag acgccgccga cgggacccat cacacgggcc agcagccggc tgaacggcga
    16681 ggccatcgtc gggccgatca ccagggcgaa cgacaccagg accatcaccg ctccgacccc
    16741 gaccatggcg acggggctcg gaccggtgtc ggccagaccc aggccgatca gcgccccgcc
    16801 gaccaggacc atgagcaccc cgacgaccat ccgggaccgc gacaggttcg agcggtcgat
    16861 cgacacctca ccgagggcgg cgatcggtgg tacccgggtg gaacgccagg ccggggcgag
    16921 ggccgacacc accgtgatcc ccactccgac gaccagggcg aacacgacgt tgccgacccc
    16981 gggaagggcg ggtgagccgg ccatggtgaa caggttctgg agtgcgttga ccaggagcat
    17041 ggcgatgatc acgcccaaca ccagtccgag gaccgacgcg atgacgccga cgacgaccgc
    17101 ctcgagcagc gaggcgccga gcacctggcg acgtcgggcc ccgaccgctc gcagcagcgc
    17161 cgtctcccgg gtgcgctgct gcaccaggat cgagaacgtg ttgtagatga tgaagatcgc
    17221 gacgaacagc gcgacgtagc cgaacacgct gacgaaggtg gagaggatgt cgacgaactt
    17281 ggagatcgac tcctggttct catccacgaa ctgccggccg gtgatcacct gctcctgggg
    17341 gagggcgtcg gccagcgtcc gcgcgatctc acgttgaccg atgccgggtt cggcccgcac
    17401 ggccacgaag ttgtactgac ccggttggcc ggtgatcgcc tgggcctcgg cggtggtgaa
    17461 gaagatcggc ttggcgccgg tcttgccctc tccgtcggcc cccaggccga cgacgccgac
    17521 cagcgtgtag gtcttcaccc cgccggtcag ggtcgcgacc ttcaccggat cacccggctc
    17581 cagcccgagc gtgtcggccg aggtgccgtc gatggcgatc tcgtcgtccg cggtcggccc
    17641 gcgcccctcg cgcacgccgc cggtgcccat gatcgagtcc tcgacccagt tgtaggtgat
    17701 cgtcgggggt ccgaccccgc cgccgaacac cttgcccttg gagtcgatca gctcgttgcc
    17761 ggccgcctcg acgaagccct gggcccggtc gacgccgggg acgccctgca cctcgtccag
    17821 caccgttgcc tccacggggg tccgcagcgg ttggccgaac ggggtgctgg tggtcttggg
    17881 ggagcggacg acggcgtcga cgccctggta cacgtcggcg acgagcgagc ggatcgagtc
    17941 gttgagggtg gtcgacagca gctgcgtccc ggtgaggaac gcgatgccga gcacgacggc
    18001 gatcgacgtg gtgaccagcc ggcgcttgcg cgccatcagg tccttgaggg cgacgcgcag
    18061 cacggggtca gtccccgagc tgtttcatca ggtccagcac cccggccgcg gtcggatccg
    18121 acatctcggt gacgatgcga ccgtcggcga ggaacacgac ccggtccgcg tgcgcggccg
    18181 ctccggcgtc gtgggtgacc atcacgatcg tctggtggag gtcggtgacg gcgtggcgca
    18241 tgaactggag gatctcgccc ccggagcgcg agtcgaggtt gccggtcggc tcgtcggcga
    18301 acacgatctc gggccggctg gcgagcgcgc gggccaccgc cacccgctgc tgctggccgc
    18361 cggacagctc gctcggtcgg tgcgacagcc ggctgcgcag cccgacggtg tcgatgacct
    18421 ggttcatcca ggtcgtgtcc gggtcgcgcc cggccaggtc catcggaagg gtgatgttct
    18481 ccgccgccga cagcgtgggg atgaggttga aggcctggaa gacgaacccg atcttgtcgc
    18541 ggcgcagctc ggtcagctgg ccctcggaga ggtcgtccag gtacgtctcg ccgatgaaga
    18601 cccgaccgga actcagtcgg tccaggccgg ccaggcagtg catcaacgtg gacttgcccg
    18661 aaccggacgg gcccatgatg gcggtgaatc ggctgcgctc gaagctgacc gtcaccccgt
    18721 cgagggcgcg gacctcggtg tcgccctcgc cgtacacctt gtgggcgtcg atggcgccgg
    18781 cggcggcgac ctgggtggcg gcgggcgggc cgggctcggg cgagcgcggc gaggggccgg
    18841 acgtagctga cacgggtgct cctcaggacg ggatctgcac actacccgtg gcccgccgag
    18901 ggccgtcgtt cattcggcgg ccacggtgga gccgcacatg cgctgtccca acgggccgag
    18961 atgggcggcg agcaaccggg tccagtggtc gcggacctcg gggcgggccc gacaggcgac
    19021 gcggaacaac cgacgggcgt cgcgcccctg ctgcagccgc acggctgcca tcgcccctgc
    19081 ggtcgcgtag cgggcgagca gccgttcgtc ggggatcggc gtccgcgcct gggcgtcgat
    19141 cccctggagg gccaggtgga actgcatctc gtcgtcggtg cggtgagcgg tcgaggggtc
    19201 ggggcagtag tcgtgccacg acaggaggag ctccgggctg tggaggacga ccgctccgtc
    19261 gagcaccgcc agatccagcg cgacccggcc gaagtggccg gcgggatcga cctcggcggc
    19321 gtggccccgg ccggcgtcga gctcgtctcc gatcaggtcg gccgcgctcc ggaggcggtc
    19381 ggtggtggtg gcgaacgccc cggcgcggag gcaggcggtc ggcccgccgc cgtcgacgac
    19441 ggggcggaac tcgctgatcg tcccccgcgg atcgatctga cgtcctccgc agctgacgaa
    19501 gccggccccc gacccgtcgg ccaggcggcc gatgcgggcg agccatgcgg cgtcgacgtc
    19561 gacatcggcg ttgatcaccg tcgcccaccg gccccgagcc atgccgagag cgtcgcggta
    19621 ggacggctcg gcggtcgcgg tgacgtcgtg atccggttcg aagatgacga cgcgatcgtc
    19681 cgcgacggcg cggaccgcgt cgagcgtgcg gtcggtcgag ccgaggtcga cgacgatcag
    19741 ctcgaggtcg ccgaaggtct gcgcgagcac gccggcgacg gtccggccga cgacctcggc
    19801 gcggtcccgg accgggagca gcacgctgaa ccggggatga cgcacgtcac catgatgggc
    19861 cgtggcggtg ccgcgaccgc ggaccgtcag ggatcgcggg gcagcactcg gtacaccgac
    19921 acgtcaccgt cggcggccag cggttcgagc tcgacccgtc ggcgcagctg gtcccgggtc
    19981 cacaggtggc cgtaccgggg ctgctcgtcg acccagacca ggcacgtcgg ggtgcgcgcc
    20041 aaggtgggga ccaggcggtc gaggtcgtcg accggatcgc tcgactccag cccggtgcgt
    20101 cgggggctcc actccgactc catcgtcggg tacagggcgt tgggcaggtt ggacaggacc
    20161 cggcagccct cggggaccgc cgcgacggcg gggttggccc ggacgcgatc gaaggtgtcc
    20221 gacgagtagt tgccgtcgaa gtacgggttg cccatcgcga acgccaccac ggcgacgatc
    20281 cccgccgcga tgttggctcc cgcccagacc ctcgccacga tcaggccgcg gcccggccag
    20341 gccggaccga gggcgccgag tcggtcgacc aggagcagcc cgcaggccat cagcggcagg
    20401 taggccggtt cgagcagccg caggtcgagt cggttgagcg ccgtggtcga ccgcacgtac
    20461 agcatgtagg tcaggtaccc gacaccgagc agcaggagca gcccggtggg tccgccgagc
    20521 agtcccagga cccgggcggc gacgctctcg ccgtcgagcc gggtggcgac gagcaaccgg
    20581 acgccgagca ccactgcggc gatgacggcg atcacgccga cgacggccca cagccgatcg
    20641 cgtccgttgg cgacgcccgg cagcaggaac ttcccgacgg tggcgacgat gtcgacggcg
    20701 ttgccgaccg ggccgcgctc cgacgggtag cgggggccgg tgaaggtgcc gtcgagcgcg
    20761 tggttgcgca acatccacgc cagcggggcc agggtggcga ggacggcgaa gccgccggcc
    20821 tcggcgacgc ggcgcccgaa ccgccggggg gtctccgccc cggggggcgg caggatcagc
    20881 agccacagcg ccacgatcgg gatcaggtac agcgccgcat agcgaaggaa gaacgacagg
    20941 ccgacgagcg cagccgtcgc gaccagtgac cagatgtcac gtcggtcgcg gaagcgcagc
    21001 agcaccagga cgacggcgac gaccacgacc gagaaggcga agtcggtcat cagcaggtgg
    21061 ccgaagccga tcgtcgagct gccgagcgcg accaccgcgg ttccgagcag gaccaggtgg
    21121 tcgtcgtcga gcacgcgccg cagcagccgg tgcaccagga cgaccgcggc gacggtcaca
    21181 gcggtgttga gggcgatggc ggctccgacc ggatcgagcg gggtcacctt ggcgaggccg
    21241 gccatcaacg acggccacag gggcggccac acggtcagtg ggtgctcgag gaagtactgc
    21301 aggccgtcgc cgcgcagcag cgagtcggcg gtggcccggt agccgacccc gtcgtcgccg
    21361 accgcgatgc ctccgaggtt ggacaccagc accagggtcc cgctgagggc agcgagcggc
    21421 agggcccagc gatccgatcg gcgtgcggcg tcgtggtggt cgtgcatctc gtcggtggtc
    21481 gtcactgcgc ccggtcctgc ccctcgcgtc gacgcgtccg ccaccagccg gtcacggccg
    21541 caccagtgtc cgatgccgtc gaaccggaca caagtccggc gctgccctcc gttccgcccc
    21601 cgttctgtag tgcgaggacc aggcgaacgt gtggatgtcg cactacagaa cgggggaggg
    21661 gcggagcgat gggggaggcg accgtatgct gggaccgatg gactgcccca gctgcggcgc
    21721 cgcgggtgcg gcgggcgccc ggttctgctg gtcgtgtggt cagccgctgc gggccccgac
    21781 cgaggaacgc cgggtcgtca ccgtgctgtt cgcggatctg gtcgggttca ccgcgctgtc
    21841 cgagcacctc gaccccgaac aggtccgacg actggtcgac cacgccttcg agcggctggt
    21901 gcgcgacgtg acgtcgttcg ggggtcgggt cgacaagatc gtcggcgacg cgatcgtggc
    21961 tctgttcggc gcgccggtcg cccacgagga cgacgccgag cgcgcggtgc ggtccggtct
    22021 gcggatgcag gagaccctgg cggcgatggc cgacgacgac tggctgcccg atgcgggttc
    22081 cccgatcggc ccggacggtg ggcccgtccc ggcgttgcgg atgcggatcg ggatcaacac
    22141 gggcgaggtg ctcgtggggg cgttgcgggc cgggggcgac tacaccgcca tgggcgacgt
    22201 cgtgaacacc gcctcgcgcc tgcagacgat ggccgaaccc ggagaggtcc tggtcggcgg
    22261 cagcacccac gccgccacgg cgtcggtcat ctcctacgag tcccggggag cgctggtccc
    22321 ccggggccgc gacggagccg tgccggtgtt ctcggccatc acggcggtgc agcctccggg
    22381 ctaccggcct cgcgcccggg tggaggcgcc gctggtcggc cgcggtccgg agatggacgc
    22441 gctcaccgac gccctgggct tgtcggtcgc ccgagggcgc ggtcagatcg tcctgatcgt
    22501 gggcgaggcc ggtgtcggca agacgcgcct ggccgacgag ctggcccacg tggcggcgat
    22561 cgaccacggc gtggcccact tcaacggtcg ttgcgtcccc tacggcgagg cgaacccgtg
    22621 gtggccggtc gccgaggccc tgcgcgccgg ctgtggtgtc catcgcgacg atccgctgac
    22681 ggtggcccac gagcgggtga cccgggcggt ggcgacggca tgccgaccgc ccgaccccgg
    22741 gtcggctgcg ccaccgaacg agctgaccgg cgactcggag gaggcgatcg tcgccggcct
    22801 gctgcacctg atggggtacg agggcccgtg gcgggggctc gacggtccgg cggtgcgcgc
    22861 cgaagccacc cacgcgttgg tgtcgttcct cgaggcgtcg ttgcgccacg gcccgatcat
    22921 ggtgcgggtg tcggacctgc actgggccga ccaggtggtg ctcgacctga tcgacgaggt
    22981 ggcggcccgt ctggcgcgct gtcccttcct gctggtggcc accgctcggg gcgagctcca
    23041 ggagcgctgg agcccgaggg cgggccggtt caacaccctg gtgctcaacc tcgaccctct
    23101 ggaccggacg gcctcggcgc agctgctcga cctgttggcc ggcgccgagc tcgatccgca
    23161 gcggcgatcc acgctgctcg accgatccgg tgggaacccg ttctacctgg aggagctcgt
    23221 cactctggtc ggacccgctc cggctgccac cgtgggggac cctcccgcgg gtgttcccgt
    23281 cggtgccgta gatgacgggt ccgaccgacc gggagcgacc gggtcggcgt cgcgatccgg
    23341 acccggcgtc gggcgtgggg agctctccgg gccggcctcg gacgccgctc tgcccgacac
    23401 gctgcgcgga ttggtggcgg cacggatcga cggcctgggc cgcgacgagc agcaggtcct
    23461 cgaggacgcg gcggtctggg gggcctcggg caccgtcgag gtgctccagc ggatcgcgac
    23521 tgccatgcga tccggagagg acgtgacccc cgtcgtgcgg aacctcgccg accaggacgt
    23581 cctcgtcctc gacggcgagg agtggtcgtt ccgctccgac ctgatccgcg aggtcgccta
    23641 cggacggctc accaagcgcg agcgtctgat ccgccacctg ggcatcgccg agttcctcga
    23701 ccgcaagttc cgcgaggccg acgcggacga cgggaccgtc gacaccgtca cccgccactt
    23761 cgtcgaggcg gcccgactcg accgtgacct ggggcgtgcc catcaggagc gggcggtggt
    23821 ggcccacgcg gtccgctggg cgctcgaggc ggcccgacgg gccgagagcg acgccagctg
    23881 ggagctggcc cgacgtctct acacccaggc gctggatctg ctcgacgacg agtcggaccc
    23941 ggtcgcggtg gagagccgct tccgttgcct cgtggggcgg tcccgcgtgc ggtccgaggt
    24001 gtgggacgag gccggggccc ggcgcgacgc ggccgatgcc ctggaactgg ccgacagtct
    24061 cggtgacccc gcggaccggg cggtcgccct gctccggctg ggaaccgccg cggctcgtgc
    24121 cggtgactcc gaacgggccg aggtcgagct gtcggagtcg atccggatct tcgaggagac
    24181 gggcgatctc cagggtcagg ccgaggcgct gcgcgagaag gggttggccg ccctgctccg
    24241 cggcgattcc cgcggtgccg aggacccgat cgcggcggcc ctggacgcct tccgctccgt
    24301 cggcgaccgt cggggcgagg cctggtgcct ccagaacctg gcgtggatcg ccctgtcgga
    24361 cggccggccg gtcgccgccg ccgagttcgt cgccgcctcc gaggaggcgt tccgcgaggt
    24421 gggcgatcgt ggggggctgt cgtgggcgca gggactggcc gcgttctgcc gcctgggcga
    24481 gggcgacctc gacgcggcgg gcgagatcgc gggtcggatc ctccgcgaga gcgagcgtcg
    24541 cggcgaccgg ttcggcgtgg ggatgatgct gaccgccctg gcccacgtcg agctgtggca
    24601 gggccgggcc cgggccgcgg cggcctcggc ggagcgggcg gtgacggcgt tcgcgtcctc
    24661 gaccgagctc gccggcacgg tcgggctcga acaggcgatg gccatcgcgg gtcgggccga
    24721 gatcatggcc ggcaacaccg ggaagggtcg gcacctcctg gccgaggcgg tgcgctccgg
    24781 tccaggtggt gcgccgagcg tgtacgcgtc cggcgtcgcg ctggtgaccg acgtccagct
    24841 gggtcggccc gagcgggtgg tcgcgttgga tcccgacctc tggagcgtgg cggcgggctc
    24901 cgggcgccga ggggaccagg acgcggcgct ggcgctggcg ctggctcagt ccggacgtgt
    24961 cgccgaggcg gtgcggcgtg ctgacgccgc cgtcggaacc gatccggacc acgggtacgt
    25021 cgtggcgtgc gcggcgctgg tcgcggccgc ggccggagat cacgagcggg ctcgcgagag
    25081 ggcgctccgg gtgctcgggc tggcccgggc cacctatctg gacagggtgt gggcccggat
    25141 cgtcctggcc ctggtggcgc cgggggaggg tggggtcgac caactggacc tcgccgacgg
    25201 tgagatcggt ccgggcgagg acgtggtggc ggtcgcgctg gtcgacctgg cacgactggt
    25261 cctcgacgcc cgaggcgagg accgatggcg acaggccgac ggggtcgagc ctcgcgggct
    25321 ggacctcggg ggatccgagg tcgggcgcag gctgtgggag ctcgggctcg acgacacccg
    25381 ctgggccgac ctgttcgtgg ccgcggtcac cgctggcgac ctggcggctc ccgctccgtg
    25441 acgcggctgc gggacgagcc cgtgggcccg ccccgcagtc gaggtcgatg gagctccgct
    25501 gcgcgtcagc cgggctgggg cacgtagcgc aggtagggct tctcctcgcg ccagccgtcg
    25561 gggtagatcg acttggcctc gtcgttgggg atggccggcg agatgatgac ctcgccgccc
    25621 tcggtccagt tggccggggt ggccaccttg tggttggcgg tgagctgcag cgagtcgatg
    25681 acccggagga tctcgtcgaa gttgcgcccg gtggaggcgg ggtaggtgag gatcagcttg
    25741 accttgtcgt cgtcgccgat gatgaacaca gagcgcaccg tcagggtgtc gttggcgttc
    25801 gggtggatca tgtcgtagag gtccgacacc ttgtggtcgg ggtccgcgat cagcgggaag
    25861 ttcaggggca cgccctgggt ctcctcgatg tcgccggccc agccccggtg gtcctcgatc
    25921 gggtcgaccg acaggccgat gaccttgacg ttgcgcttgg cccactcgtc cttcagcttg
    25981 gccacggtcc cgagctcggt cgtgcagacc ggggtgtagt ccttggggtg gctgaacagg
    26041 acggcccagc tgcccttctt ccagtcgagg aagtcgacgg ttccctcggt ggtctcgatg
    26101 ctgaagtcgg gggcgctgtc gccgagtcgg atggccatct gcggatctcc tctgatcgcg
    26161 tgattgattc cagaccaaac tagtcgggta ttcggacggc cgacaacgcg cccgctgggc
    26221 gggcacgcgt cgaggcgccg gccggtcccg cctcgatggt tgcgaacatc tgcaagcaat
    26281 cccttgacgg agttcgcagg cgcttgctac agtaagcacc gaagagcggt gagcaaccac
    26341 accgcgccga tcccgacatc ccgaccggaa ccggacggga tcagaccgtg gaggtcaacc
    26401 atggacaacc tgctcgatcc cgtcaccgac atccaggaca aggtcgtcga cggtgtgaag
    26461 cgcaccaagc agccgatcgc cgacgccgtg gcgaccgtcg tcggtctggt cctcgaccag
    26521 gtcccctcgg tcccggccct gccctacgcg gagctgttcc cgaccccgct cgaggtcatc
    26581 gacaaccagg ccaagttcgc caccaaggtc gtcaacgcca ccaagaccgt cgccaccgcc
    26641 gcggccaagt cggcagctcc tctgaccgat cagctcctcg accgcaccgc tccggtgtcg
    26701 gcggtcaagg cggcctgatc ccacagatct ggtccggaac tgtcccccct ggttccggag
    26761 accagtcacc gccacgacac ccccctggcg tgagcggttg agagcggcgg gcccgccgct
    26821 ccgcgttcaa actgcagggc tgagagcgcc gggacgggcg gggaaccggc ggatccgggt
    26881 cagtccgtcg tcgccgacgg cgcggccggt gcggtggggt cctggtgccg gtcgccgtac
    26941 cgggccgact tgcggcgtag gaacggcgac tcgatgaacc gccaggacag ctcggcgacg
    27001 cccatgctgg cgacgaccag gaccaccagt cgcaccagca gggccgccgt gcccgcgggc
    27061 ttgggggtga ggatgttgag cacctcgtgc cagaggtaca gcccgtaggc gcgcttgccg
    27121 atccacaccg ccagcgggag cgacagccac cgggccgggc cgacccgggg gttcaggtgg
    27181 atccacagca ccgcgaaagg gatgccggcg agcgccaggt tgaacccgat gctgccgaac
    27241 gggtcgaagg cgccgacgcg gccggcgagc aggagctcgg ccaccagcac gagcgtgccc
    27301 agcgccgccg cggcgtcgag ccatcggcgc cgcggagcgc ccggctcgga cgcgaccagg
    27361 gcgtagcggc gcacgaccac ggccagcagg cagcccacca gcagctggtc gaagcgcatc
    27421 gccgagaacc ggtacaggac ctcaccgacg tacgtcaccc cggtcccgac gaacggatcg
    27481 tcgtgggtcc agttcacccc gaccgcctcg cccgacaggc gcacgagcac ccagaagccc
    27541 gcccccgccg acgcggccgc gatcagcgtc cgctggcggg cacgtgagcg caacccgagg
    27601 agcagcagga acggccagaa gaagtagaac cactcctcga ccgacaggga ccacaggtgg
    27661 aacagca
//
LOCUS       DAOQOD010000018        27633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_562_length_27633_cov_11.493836, whole genome shotgun sequence.
ACCESSION   DAOQOD010000018 DAOQOD010000000
VERSION     DAOQOD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_27633_cov_11.493836"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLZ72_03415"
     CDS             complement(<1..514)
                     /locus_tag="PLZ72_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA35380.1"
                     /translation="MSAQLPSRDEEILGRPAVDDLEAILTTANTDVDEVIHAVRDNAD
                     VIFTWDYEKGARPALNKLYEKAKGSQWNGQTDLDWSIDVDQQQFAEMMADMQQMEFEA
                     RGVDFTGTPFEKWKRAEWVELAHEVQNWSLSQFMHGEQGALLCTAKIVETVPWIDAKY
                     YAATQVMDEAR"
     gene            complement(712..915)
                     /locus_tag="PLZ72_03420"
     CDS             complement(712..915)
                     /locus_tag="PLZ72_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35381.1"
                     /translation="MTVPTTVEAAPAENTTIVTGRARRLRARRRNVALGSIVAGAAVF
                     SSGCTLGDVSTILSILKLLGIIS"
     gene            1089..2786
                     /locus_tag="PLZ72_03425"
     CDS             1089..2786
                     /locus_tag="PLZ72_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35382.1"
                     /translation="MDGDRQTPGWFDTHEVTNQPPPLEAYDVFSSDRALVEAVERHGA
                     AHNVGDLAAIGRRAGDPEWIERGRQANSVVPTLRTHDRYGHRLDVVQYHPAYHDLMEA
                     AVEAGLHAAPWVDTRPAPHVTRAAGVITWYQVDAGHTCPISMTYSSVPALGHEPELAA
                     DLVPRLTARDYDGSNRPINEKTGITAGMAMTEKQGGSDVRANSTVARANPAPIGVAGR
                     EFLITGHKWFCSAPMSDGFLTLAVAPGGLSCFWMPRWRPDGTRNAVRIQRLKDKLGDR
                     SNASSEIEMLDAWAVMVGDEGRGVRTIIEMVNHTRLDCTLGSAAIMRQGTAQAIWHAA
                     HRQAFGRTLVEQPLMSNVLADLAVESEAATVAAMRAAATFDAADDPAEALLARLVLPI
                     VKYWTCKRATTHAAEAMECLGGVGYVEETDLPRLFRQSPVNGIWEGSGNVMCLDVLRA
                     MMTSPESIEAYWAEVDLGCGSDRRLDDAAERLRKELADFDDIEVRARRIVEALALLLQ
                     GSLLVRHSPSAVADAFVASRLDPDRSARSAFGTLPAGTDLDAIIERHRPSVTVTAAP"
     gene            2796..6335
                     /gene="cydD"
                     /locus_tag="PLZ72_03430"
     CDS             2796..6335
                     /gene="cydD"
                     /locus_tag="PLZ72_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HNA35383.1"
                     /translation="MLEPVRPVVDGEDPLDPGTHRPRRGPIDPHLLRLAPSLRRHLAI
                     CGALAVVTAVAVLAQAEIIGRHLPALIDGDRAGAPTLAAALVGIGLIRFLLRWATELS
                     SSRAAAGARATITDRVISHALAIDEPGAGVATPSRVTTLVTDGTDALEPWIREYVPAL
                     CIAGVLPLAAGARILAADVPSGLILLVTIPLIPVFMVLIGKLAEDRADRQWATLQRLA
                     AHFHDVLVGLPTLRLFGRVGPQVQRVREVAEQYRVAVMRTLRVAFLSAAAMELLAMLS
                     VALVAVTIGYRLSVGTVSLQAALVVLLLAPECSLPIRRVGSAFHAAQAGTDAADELRD
                     LLATTTTPDGPVDTLAEPAPATPVLRISGAIVADPQRGVRVGPVDLEVHAGSLIALVG
                     ASGAGKSTLLDAVRGRVPVHRGRVEVAGIDVAQLSRTTRTDAIVWIPQLPDPVGADVR
                     AAVVAGSDPADAEAIDRVLDAVDLVGLADRDPTELSGGERQRLAVARGVLRSAASPEV
                     RLVLADEPTSQLDGNRRALVIDALRRMASEGLAVVVATHDPVLIEAADHVARLGEELP
                     PPAATGRVAAPVAPDVAEPTTPQGDPTRSRSSRPSPPNGQVVDQVVDHHAGHNAELTR
                     PEDDLIWFRRMSRPVRWRLRSGRALAVSTDLCGIALAATAAWMLAKASMGTAFAELAV
                     AAVLIRVFAIGKGLLRYSERLVSHDATFRMLADVRGAVVARLGRIAPAGVPGMERGDV
                     MARVVDDVDRLADQELRVTGPMTSGVIVGMVAVAAAAVVAPGFGVVYAAAVVLLAIVL
                     PAATRRSTAGAVRDQLAQRTRLAGAALELVEHSDELTATGASDLWSARIGDAVQSLGA
                     AERRRGRRIGVLGGLAALAAPSVAAAVVLVDGTANGTVDGALLAAVVLAPFALVEVLV
                     PLLHAGEQQPSIEVAATRLRAVLDAPDPVVDPEDPLAVPAHPSVVLDAVSLRWPNTTD
                     PVLRGLALHLDPGSRAEITGPSGTGKSTLAAAMVRFIGVDGGRYLLDEIDTADAEGDD
                     VRSVVTWCQQSPWLASTSLRENLRIAAPDAGDDELWAALDAVHLSDWAVALPGGLSSE
                     VGRDGDAMSGGQRQRLALARVLLAGHSIVVLDEPTAHLDGPTAERVRADLLAALDGRT
                     VILLGHDRASGDDGPTAIDGRWMHVDLSRSSSV"
     gene            complement(6387..7343)
                     /locus_tag="PLZ72_03435"
     CDS             complement(6387..7343)
                     /locus_tag="PLZ72_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNA35384.1"
                     /translation="MQLRQLEHFEAVYRLRSFTRAARELYLSQSALSRSIQALEADLG
                     QTLFDRSTQAVEPTDAAEALIGHAVDAVAAARTLVESARLLRDGEGGSVAIGTGPYPA
                     RPLMTRAVTALSRERPGLQVAVVGGGASDLLASLVRRDLDFVVCDTSKAEESSFAAEI
                     DSEPLPAEPLALVVGADHPLAGIPEPSPAQVTAHPFVLPPPAPVGRRVLTRSLDPEHR
                     ATRLPFYEVESTAACLELVADQRSVTLVPVSLARQECPGRGLLFRAAGHRQATHDGIH
                     VLRGRTMGASARIARDAVLSQANAIAREHRSWQRAAGPGWQR"
     gene            7452..9095
                     /locus_tag="PLZ72_03440"
     CDS             7452..9095
                     /locus_tag="PLZ72_03440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNA35385.1"
                     /translation="MTSSTPPVPGSIDLRDRSRAFADLDRETFDVVVIGGGITGAGIA
                     RDAALRGLRVALVEARDIASGTSSRSSKMIHGGLRYLAQGDVGLVREAATERQVLRRI
                     APQLTRLSPFVMPGSRSMIAKVRAGLVAFERLGKVPRDERHRTLDATELLELEPTLAA
                     NRFSSGVVYPEFLTNDARLTVANVRSAVASGATVLTHAAATRITVGSNGRADGVEVAG
                     TLPGEDLAARARTRLVVNAAGPWVDAVRALEAPDEPARLALTKGIHVVVPHRRLPVRD
                     TVITLGPDKRPVFAVAAGPVTYLGTTDTFYTEPDEWPRIDASDVQYLLDAMEQTFDGP
                     PISPDDVVSLWSGVRPLIAPPEGSDAAPSELSRRDETWVGPAGVVSVAGGKLTAYRTM
                     AERIVDDLVGDLGTGSLRCRTADVPLIGGDVDVGDLTAALGSSLGEDAARLVQLWGSD
                     ASSISDDGGDVAAEVRFAVCHEGALRLEDVWIRRAARSWFTLEPVGTDLDVAAATMAD
                     LLGWTPERQAAEVAACHTIHHDARRCLDELADGAPTPVA"
     gene            9172..10599
                     /locus_tag="PLZ72_03445"
     CDS             9172..10599
                     /locus_tag="PLZ72_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA35386.1"
                     /translation="MLDSTIDTLRDLLGTDVVSTDAEVLASRRRDYWAASALREWRGD
                     TMGEPGCVVRPRGPEQVQDLVRFCGANRIALMPFGLGSGVCGGVLPTDDVVLLDMSSM
                     SRTREIDEHDLLATFDAGKNGLEAEEEVAERGLTIGHWPQSIAVSSVGGWVSTRASGQ
                     FSTAYGNVEDLVYSIEAVLPDGELVHLGKAPRAAAGPDLRHVVMGAEGTMGVVTGVTF
                     SLRRAPESRRLTAFHSPGAAPTDSMALGIEVQRRIVQSDWRPPVMRLYDGSEVARNFP
                     EHRSGDDAALLMVHEGPAGRVDAEVADVTRIATEAGLTVADAGMVEHWLESRNHVPTW
                     TDFFERGAVVDTIEISAPWSRIVDVYAAVVASLGQVEGIVNASAHSSHVYRSGINLYF
                     SFAVLAEVADMEDRYFECWHRTMEATAANGGGIAHHHGSGRLRIPYLHHDLGAGGVQL
                     LRTLKGAVDPAGIMNPGNLIPSPPR"
     gene            complement(10667..11323)
                     /locus_tag="PLZ72_03450"
     CDS             complement(10667..11323)
                     /locus_tag="PLZ72_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35387.1"
                     /translation="MMLSQLSDPPDSGTEAPLGGPGDGPVDGRSARRERGRAAAVQAV
                     VELFDTGELRPSADVVCRRAGVSAASLFRYFRTLDELYRAAFDEQIARATALARMEDL
                     SDLSTSERIERFVAGRLDVYEVTVGVGRMARARAIDHADIARALGRARARWLRQVLIV
                     FRPELDRVDPAEVPSRAAAIDAVASFEAWDLLVGARAVSRPAVERSWTTAIAALLAAL
                     "
     gene            11411..13696
                     /locus_tag="PLZ72_03455"
     CDS             11411..13696
                     /locus_tag="PLZ72_03455"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNA35388.1"
                     /translation="MRTERFEGTIGATLAESTPDFDTPPDPGPGAPDVLVIVLDDTGF
                     AQLGCFGSDIDTPNIDALAADGVMFTNFHVTPLCSPTRAALLTGRSHHDAGMRGLSNF
                     RTGFPHMLGHLSHGTATMAEVLSDAGWATFAVGKWHLAPMEECSAAGPFDQWPLGRGF
                     DRFYGFLDGETDQFHPSLTVDNHHIDPPATPEDGYHLSEDLVDRALEMITASTNIRPD
                     RPVFTWFALGATHAPHQAPAPYRDKYRGRYDEGWDVVRERWFERQKELRVVPPHTELS
                     DRNPGVEAWESLPDTERRLAARLQEAFAAFLDHTDDQIGRLLDGLRDLGRLDNTIVVL
                     VSDNGASQEGGPYGVMHEMKFFNGILETPEQAIGRIDDIGGPHSHTNYPWGWAQCGNS
                     PFRWYKQNTHEGGVHVPFILRWPRGLADQAGTTRRQFAHASDLAPTLYELCGVDAPET
                     YRGVEQRPITGRSFAPVLRDAASPATNTVQYFEMAGSRAIVRGGPDGELTKAVCKHDK
                     GADYDTEPWELYDLLADPAEGRDLAAERPELLGELVELWWSEARAHRVLPLDDRLIEL
                     FGVRFRPLSPHPESRRYVYRPSPWPMPAQAGAAIGGRDFDMTATVVREAGQDGVLFAT
                     GTENSGLSLFVQDDRLVLDYNAFDDHTVVVSDAPVPAGASVVELRVRRVDHSSGTAGL
                     LIDGSPSGSAELPLLMRIMSSVGASIGHDHGSAVSPRYQAPFPFQGTLTELVIQLSPD
                     RFGGDSDPTTAAEAAHEMSRQ"
     gene            13751..14677
                     /locus_tag="PLZ72_03460"
     CDS             13751..14677
                     /locus_tag="PLZ72_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HNA35389.1"
                     /translation="MNIVRTPDSRFEALPDFPFEPHYAEVPTLDPEDADGTTLRVAYL
                     DEGPRDAAPVLLMHGEPSWSFLYRHVIPGLVARGHRVIAPDLVGFGRSDKPTAPSDYT
                     YARHVAWMEDLIVAQLDVRDATFFGQDWGGLVGLRVVAAHAERFARLVVANTGLPEGT
                     GRITEAFLAWQKFSQESEDFPVGAIISGGCVDRLAPEVVAAYDAPYPDDSYKAGARIF
                     PTLVPTTPDDPGGLANQAAWKVLETFDRPVLCAFSDSDPITKGGAKPFLERIPGARGR
                     SHTTIEGGGHFLQEDRGPELATVIADFIDETR"
     gene            14745..16445
                     /locus_tag="PLZ72_03465"
     CDS             14745..16445
                     /locus_tag="PLZ72_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNA35390.1"
                     /translation="MDSAPTSTSTATSTVTDPEAEVRRDAAHLLGLSEWDPAAVDPGI
                     LTTVTSERNGTAATAARMRRYIRHLLDHPVSHVPDRSELWRTMLEHCERLTPVQAYTL
                     RNLMGPTSSPAGDGGYAPMPPEVHLEFPRDDAVDLGAQCGWHFLVGSLWDAEGNEYGI
                     EFMLFEQAMYPPAFARELGLSPLDNLVAEVQFAISTRGDRHHQAEPLVTLGTSGLIRT
                     GTSPFSFRVGANSFESLERDSLMPMRVRSVGVDLGGEQPLPLSCDLVLTGGKAVLEQG
                     DHGAMPSVAGIGTYYYSIPRIDIDPQQSSISIDGRQIPVVGGELWFDHQWGFLSGTSP
                     DPVLRASDSITAPNPGGWDWFMTHLSGDRQVTVFAPHSNEFAEFYGCTGDTPPPTMTR
                     RVGGTFMDPNANTRLVWGTVHVDRWVKVMHTPNPQRYPATATWYPNHYRFEFDDLPDD
                     ISELTLTPIVEGGQSAFFSHGVQICEGAVVVSDRDGNDIGRGFAEAVAFADTLRNRLR
                     LAGLPDTDEMAALAAEPTPSPELAAANAAFVAAHPDQMAAIVAAAKGLEFFMEPSSEA
                     "
     gene            16494..16907
                     /locus_tag="PLZ72_03470"
     CDS             16494..16907
                     /locus_tag="PLZ72_03470"
                     /inference="COORDINATES: protein motif:HMM:NF021510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNA35391.1"
                     /translation="MDTDDDVERNVCGGPLQECGTEPLTGFFRDGCCRTGPDDLGSHT
                     ICAVMTPEFLAHQRSIGNDLSTPLPQYGFPGLVPGDRWCVTARNWLRAHHDGVAAPVV
                     LGATHQAVTELVPFEVLWDHRIDVPDDPGPLIDPF"
     gene            complement(16957..18336)
                     /locus_tag="PLZ72_03475"
     CDS             complement(16957..18336)
                     /locus_tag="PLZ72_03475"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HNA35392.1"
                     /translation="MDTSRRRLEPLIGGGRTVLTRSLADVRLRDVGYEQTEWACSGHA
                     ESYRAPSGLPTDGRWTFEVRDHAEFTTRVLIRRPSDEGRFSGTVVVEWLNVSSGSDSA
                     PVFGFSGPELTRAGHVWVGVSAQWAGIVAAPALVDVGASGLLALQSADPLRYGGLVHP
                     GDAYAYDVFTQVVDSLLDDRPEPLAALDIQSVVAVGESQSAYALVTYVNGVQPRAERF
                     DGFLIHSRGRGALPLGDAGRGADVEGSRGDPPVLIRDDLDVPVLVVQTETDVLGFLDS
                     LSCRQPDTGRFRCWELAGAAHADLSLIGELETMLGCADPVNRGQQRFVVRSALRHLVN
                     WVRTGAAPPSASPLAVAVDESGPVVHRRFETDELGNVLGGVRTPCVDVAVETLSGLCR
                     DGESHVCQLFGRRLALPDAVLRDRYPTLAAYREAYAAATDRAIDDGFLLADDRDEVLE
                     DAPADVITW"
     gene            18478..19125
                     /locus_tag="PLZ72_03480"
     CDS             18478..19125
                     /locus_tag="PLZ72_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35393.1"
                     /translation="MDTQTATPPRTQEDRRAATQQALLGATIDCLVEYGYAGTTTRLV
                     ADHARVSRGAQTHHYPTKRDLVIAAVEHLFDEYAGRFTEAFRQVPESERTLDRAVAAL
                     WELASGPSYAAILEVVVAARTDDELRSIVHGVAASLEETVVDLLLWFSPEIADRGSAR
                     RIVEFAFALIQGAAVSRMEGFGDADEVVASVAALAPFAVSLVRSGPDPTRSGATR"
     gene            19122..20072
                     /locus_tag="PLZ72_03485"
     CDS             19122..20072
                     /locus_tag="PLZ72_03485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNA35394.1"
                     /translation="MTATLSPPPTTTGRRRRRRGSRPSAIRDIPVRRLRHDQADYDLD
                     RWVVPGDPIFSHFLAILSAVFPKGEEFFVASVRRHRDAVADNPVLKEQVKGFIGQEAM
                     HSREHRRLNERLAGLGYRMARADRTIGVLVDSIYKIPPSTLPIAATAAAEHYTGLLAE
                     AVLSDESTRTTLFGSEAVEPMIAWHALEELEHKNVAFDVMEAAGGGYVVRVAGFAVTT
                     SVLASYVAIEWGRAVWEDRHLITRENRRTFHRNLMRQKLLSPWSARKLLIYLRPDFHP
                     DDTATDELVQEWRLRLAEVATANTKADTEPAPAIDPDPTV"
     gene            20166..20822
                     /locus_tag="PLZ72_03490"
     CDS             20166..20822
                     /locus_tag="PLZ72_03490"
                     /inference="COORDINATES: protein motif:HMM:NF036523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA35395.1"
                     /translation="MVQPTGPGTDRDDCDTSRAAEPTTDAQRDRRRRIVLTAMTMLQQ
                     RGYDEIQMRDVAQQAGVALGTLYRYFPSKEQLFAHVLVEWSSSFDEAVRRRRPERGSD
                     AERLRQVLRRAVGAFERHPNFLQVLTLLEVSRDPAVMEPFATYSERFVSVVTDALLDT
                     AEDDVPVLILITNSLLVTLLQGWWLGRYSIRSVHTGIDRAVDIVFEGARTDPAAPSTA
                     "
     gene            complement(20830..21663)
                     /gene="ppk2"
                     /locus_tag="PLZ72_03495"
     CDS             complement(20830..21663)
                     /gene="ppk2"
                     /locus_tag="PLZ72_03495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNA35396.1"
                     /translation="MSVDDGQRIFPPECYTKRGRLRREVYLSELERLQIELVKLQEWV
                     KATGLRVAVLFEGRDAAGKGGAINRIAEATNHRVVRVVALGPPSDRERTQWYFQRYVA
                     ELPAAGEIVLFDRSWYNRAGVERVMGFASEAEVDEFLWAVPQFEELLIRSGIILVKYW
                     FSISDEEQERRFQARIDSPLKRWKLSPMDIESRNRWEEYSHAKDEMFRVTDTAGSPWW
                     VVDGEDKRSARLNCISHLLTQVEYRDVVPDPLELPPRPAPVRTYERPPIDEQTFVPQR
                     Y"
     gene            complement(21694..22281)
                     /locus_tag="PLZ72_03500"
     CDS             complement(21694..22281)
                     /locus_tag="PLZ72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35397.1"
                     /translation="MRAVTRRAAGLVPGGWDAETRRTVAALFDGLAPDWHTRDSPQRR
                     AVVSDCLGRGLGAMAWTGGTDTVGIELGSGTGIYSDLVAGVVGSAVAVDLSDAMLREA
                     PSAPAARLRGDSSALPLCSGSVDLAVVINMFLFPGEIARVLRPGGILLWVNVSGPDTP
                     IHLTTTEVVAALPFEVDGVESTAGAGTWCALRRIR"
     gene            22514..24328
                     /locus_tag="PLZ72_03505"
     CDS             22514..24328
                     /locus_tag="PLZ72_03505"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA35398.1"
                     /translation="MTESDSPTPDGPSDEDLDAALAAARKRYQAERAKRVRSDGNAQY
                     GELTGDFAEFDRDPFADPDYHRDPLNEEVEVLVIGCGFAGMLTAIELKARGFDDVRMV
                     DRAGDFGGTWYWNRYPGCMCDVESYTYLPLLEETGYMPTEKYASAVEIFSYAQLVGRH
                     YDLYPHALFHTEVVEAVWDESTLRWRVRTNWGDTLSCRFLVTAGGILHKAKLPGIAGI
                     SRFKGRAFHTSRWNYDVTGGAPGVFMDKLADKRVAVIGTGATAVQVVPRVAEAAQELF
                     VFQRTPSPVGQRDNGPTDEAWFASLEPGWHAERVKNFTEAVTGKKPAVDLVDDGWTHV
                     MWDDTQTKSDDPEVTRQQERSDFLTMEEIRQRVLRIVEDPETAAKLQPWYGKHCKRVC
                     FHDEYLPAFNRPNVHLVDTDGHGVDEITDNGVVACGVEYPVDVIVFASGFEVTTDLEH
                     RLGFDPEGRGGIRMSERWSDGAHSLHGILSAEFPNLLMISLVQAGFGTNFLHFLSKSA
                     EHVAWIIATCEERGIATIEATPEAEEEWYELLLGVAMGIAGYSTRCTPGYYNSEQGRD
                     SKTARNLVYTGSLLEYAGFLERWRGDGSMSGALITEGT"
     gene            complement(24372..25253)
                     /locus_tag="PLZ72_03510"
     CDS             complement(24372..25253)
                     /locus_tag="PLZ72_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35399.1"
                     /translation="MNDAPVPNPTPQSERAASHLDGRVVVVTGGGGGFGRLIAEMSAE
                     RGAAVVVADIDGVAAEQVAEGIEARAGRAVGVTVDVCDPGQVEALVARAVERFGAVDV
                     MINNAGVMPLAFFADHAAAAAAWDRCIDINLKGVLHGICASYDQMVAQGRGHVVNISS
                     IYANAGTAGSGVYSATKAAVAVMSDSLRKETQGRIKVTVVRPTGVLGTGLGSGVINPE
                     AVTGITGAKAPAYMERVMAALTGELGGAAVDVDSPEFWAIEPETVAAEVIHAIDQPWG
                     VVISDVTVRATGEDYVV"
     assembly_gap    25273..25372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25373..26079)
                     /locus_tag="PLZ72_03515"
     CDS             complement(<25373..26079)
                     /locus_tag="PLZ72_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HNA35400.1"
                     /translation="MAVGLAALLDDIAALAKLAAASVDDVGAAAGKATVKAAGVVVDD
                     AAVTPAYVDGLAAERELPIIRRIAVGSVRNKLVFILPAALVLSAIAPLVVEVILMVGG
                     AYLAYEGAHKVWHRLVAHDEHPEDVPVALRGQEAEDAVVASAVRTDFILSAEIMVIAL
                     KEVIDESLVMRAAALVVVALLITAAVYGVVALIVKMDDVGLRMVEHRSAGRQRVGRGL
                     VRAMPKVLSVLSVVGTAA"
     gene            complement(26155..26841)
                     /locus_tag="PLZ72_03520"
     CDS             complement(26155..26841)
                     /locus_tag="PLZ72_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNA35401.1"
                     /translation="MTEPTPDQPDRPGGTDPAEVTGATGPPRGPGPIGGDISSIVYGL
                     GTRFRLPALTARHDSTNVMGLFALVNGVISIAIMSAAAVVTGQALIFPSLGPTAFLLF
                     YTPTVPAASPRNTICGHAIGAAAGYLALVVFGLTDAGAALATEVTWPRVGAAALSLGL
                     TSGLMVWLRVPHPPAGATTLIVSLGILTEPVQLLVLMLAVGLLVLQGYVINHLAGIDY
                     PRWAPARSSA"
     gene            complement(26834..26995)
                     /locus_tag="PLZ72_03525"
     CDS             complement(26834..26995)
                     /locus_tag="PLZ72_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35402.1"
                     /translation="MYLILLLSLQAFLLVVALEGVAAHDPALARNAAILSIVLLTSSW
                     GLWWFVRHD"
     gene            complement(27040..>27633)
                     /locus_tag="PLZ72_03530"
     CDS             complement(27040..>27633)
                     /locus_tag="PLZ72_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNA35403.1"
                     /translation="GRPVRDRGGLGMTTTDGPQNGTGSAPDLPCLGCGDDSELPLWRD
                     EFPITSSGEEHIARRDFTRYLLTASGVFAAGTVGAAAWADLQRSNLGEPRPIVDLAEV
                     PESSEYLFRYPTEEDPAILVHLQGGELRAYSQRCTHLGCVVFFQPDRDRLFCPCHDGV
                     FDPSNGDAVAGPPERPLTRIDIDVRDGVVWAKGVDEE"
BASE COUNT         4102 a        10172 c         9310 g         3949 t
ORIGIN      
        1 gccgcgcctc gtccatgacc tgggtcgcgg cgtagtactt ggcgtcgatc cacgggaccg
       61 tctcgacgat cttggcggtg cacagcaggg cgccctgttc gccgtgcatg aactggctga
      121 gggaccagtt ctgcacctcg tgggcgagct cgacccactc ggctcgcttc cacttctcga
      181 acggggtgcc ggtgaagtcg acgccgcgcg cctcgaactc catctgctgc atgtcggcca
      241 tcatctcggc gaactgctgc tggtcgacgt cgatcgacca gtcgaggtcg gtctggccgt
      301 tccactgcga gcccttggcc ttctcgtaga gcttgttgag cgcgggtcgg gcgcccttct
      361 cgtagtccca cgtgaagatc acgtccgcgt tgtcgcgcac ggcgtggatc acctcgtcca
      421 cgtcggtgtt ggccgtggtg aggatcgcct cgaggtcgtc gacggcgggg cgaccgagga
      481 tctcctcgtc gcgggacggc agctgcgcgg acatgacgga cccccgttcg acgcgacact
      541 gccgccggtg ggtccggtgc tggcgcgcct caccgacagc ctgccacacg cgatcaggat
      601 cgggtggtcc ccctgtcggc ccaccgtcgg tggtccggcc catccggcgg ggaccggcag
      661 cggctggatc ccgccggctg ccggcccccg ttcagatggt ccccggcagc ctcaggagat
      721 gatccccagc agcttgagga tcgacaggat ggtcgacaca tctcccaggg tgcagccgct
      781 cgagaacacc gcggcaccgg ccacgatgct ccccagcgcc acgttgcggc gccgtgcccg
      841 gagtcggcga gcgcgaccgg tgacgatggt cgtgttctcg gcgggtgcgg cttcgacggt
      901 ggtcggcacg gtcatggcac atctccctga tcagcgcccg gcccccacgg ccgaacgatg
      961 ttcaggacgc tacatgaacg cccgttcaag gggaaccccc ggtgtcggag atcacgaggc
     1021 gctcgacgtg acggggaggc acgcgccgac gcccaccacg ctgactcggt cggtcgtagg
     1081 ctccgcccat ggacggcgac cgccagacgc caggctggtt cgacacccat gaggtcacca
     1141 accagccccc acccctcgag gcctacgacg tgttcagctc ggaccgcgcg ttggtcgagg
     1201 ccgtcgagcg gcacggggcc gcgcacaacg tcggtgacct cgcagcgatc ggacggcgcg
     1261 ccggggatcc ggagtggatc gagcggggtc gtcaggccaa ctcggtcgtg ccgaccctgc
     1321 ggacccacga ccgctacggg caccgcctcg acgtcgtcca gtaccacccc gcatatcacg
     1381 acctgatgga ggcggccgtc gaggccggac tgcacgccgc gccgtgggtc gacacccgcc
     1441 cggccccgca cgtcaccagg gccgccggcg tcatcacctg gtaccaggtc gacgcgggcc
     1501 acacgtgccc gatctccatg acctactcgt cagtccccgc tctcggtcac gagccggagc
     1561 tcgctgccga cctcgtcccc cggctgaccg cccgggacta cgacggctcc aaccggccga
     1621 tcaatgagaa gaccggcatc accgccggca tggccatgac cgagaagcag ggaggctccg
     1681 acgtccgagc caactccacc gtcgcacgag cgaacccggc gccgatcggg gtggccggcc
     1741 gcgagttcct gatcaccggc cacaagtggt tctgctcggc gcccatgtcg gacggcttcc
     1801 tgaccctcgc ggtcgccccg ggtgggctca gctgcttctg gatgccgcgg tggaggccgg
     1861 acggcacccg caacgccgtg cgcatccagc gactcaagga caagctcggc gatcggtcga
     1921 acgcctcgag cgagatcgag atgctggacg cctgggcggt catggtcggc gacgagggcc
     1981 ggggagtgcg gacgatcatc gagatggtca accacacccg gctcgactgc accctcggat
     2041 ccgccgccat catgcgccag ggaaccgctc aggccatctg gcacgcggcg caccgtcagg
     2101 cgttcggccg caccctggtc gagcagccgc tgatgtcgaa cgtgctggcc gacctcgccg
     2161 tcgagtccga agccgcgacc gtcgcggcca tgcgggccgc ggccaccttc gacgccgccg
     2221 acgacccggc cgaagcgctg ctggctcgcc tggtcctccc gatcgtcaag tactggacgt
     2281 gcaagcgggc caccacccat gcggccgagg cgatggagtg cctcggaggg gtcggctacg
     2341 tcgaggagac cgacctgccc cgcctgttcc gccagagccc ggtcaacggc atctgggagg
     2401 ggtccggcaa cgtgatgtgc ctcgacgtcc tgcgggcgat gatgacctca cccgagtcga
     2461 tcgaggccta ctgggccgag gtcgatctcg gatgcggctc cgaccgacgt ctggacgatg
     2521 cggcggagcg tcttcgcaag gagctcgcgg acttcgacga catcgaggtc cgggcccggc
     2581 ggatcgtcga ggctctggcc cttctgctgc agggctcgct gctcgtccgc cattctccga
     2641 gcgcggtcgc cgatgccttc gtcgcgtcgc gcctcgaccc ggaccgatcg gcacgctcgg
     2701 cgttcggcac cctgcccgcc gggaccgacc tcgacgcgat catcgaacgc catcgaccgt
     2761 cggtcacggt gacggcggcg ccctagcatc gccgggtgct cgaaccggtc cggcccgtcg
     2821 tcgatggcga ggacccgctc gatcccggaa cgcaccggcc gaggcggggg ccgatcgatc
     2881 cacacctgct gaggctcgct ccgtcgctcc gtcgccatct ggccatctgt ggtgccctcg
     2941 ccgtggtgac cgccgtcgcg gtgctcgcac aggccgagat catcgggcgg caccttcctg
     3001 cactgatcga cggcgatcgt gccggcgctc cgacgctggc ggccgcgctg gtcggaatcg
     3061 gcctcatccg cttcctgctg cggtgggcga ccgagctctc ctcctcgcgg gccgccgcgg
     3121 gcgcccgagc cacgatcacc gaccgggtga tcagccacgc gctggcgatc gacgaaccgg
     3181 gggctggcgt cgcgaccccg tcgcgggtga cgacgctggt gaccgacggg accgatgcgc
     3241 tcgaaccgtg gatccgggag tacgtgccgg cgctgtgcat cgccggggtc ctccccttgg
     3301 cggccggagc acggatcctc gccgctgacg tcccgtccgg gttgatcctg ctggtgacga
     3361 tcccgctcat ccccgtcttc atggtcctga tcggcaagct ggcggaggat cgagcggacc
     3421 ggcaatgggc gaccctccag cgcctggcgg cgcacttcca cgacgtgctc gtcgggcttc
     3481 cgacgttgcg gctcttcggt cgcgtcggac cgcaggtgca gcgggtgcga gaggtcgccg
     3541 agcagtaccg ggtggcggtg atgcgcaccc ttcgggtcgc cttcctctca gctgcggcga
     3601 tggagctcct ggcgatgctg tcggtcgcac tggtcgcggt caccatcggg taccgcctca
     3661 gcgtgggcac cgtgtcgttg caggccgctc tcgtcgtgct gctgctcgct ccggagtgct
     3721 cgttgcccat ccgacgcgtc ggctcggcgt tccacgccgc acaggcgggc accgacgccg
     3781 ccgacgagct ccgggacctt ctcgcgacca cgacaactcc cgacggtccg gtcgacacgt
     3841 tggcggagcc cgcacccgcc accccggtgc ttcggatctc gggggcgatc gtggccgatc
     3901 cccagcgcgg cgtgcgcgtc ggaccggtcg acctcgaggt ccacgccgga tcgctgatcg
     3961 cgctcgtcgg tgcgagcggg gccgggaagt cgacgctgct cgacgcagtc cgcggccggg
     4021 tcccggtcca tcggggccgc gtcgaggtgg cggggatcga cgtggcgcag ctctcgagga
     4081 cgacacggac cgacgcgatc gtgtggatcc cccagctccc cgacccggtc ggcgccgacg
     4141 tccgcgccgc cgtggttgca ggatccgacc cggccgacgc cgaggcgatc gatcgggtgc
     4201 tggatgcggt cgacctcgtg gggctcgccg accgcgaccc gaccgagctc tccgggggag
     4261 agcgtcagag gctggcggtc gcccgcggcg tgctccgctc cgcagcctcg ccggaggttc
     4321 gcctcgtgct cgcggacgag ccgacctctc agctggacgg caaccgtcgc gccctcgtga
     4381 tcgacgctct gcgtcggatg gcgtccgagg ggctcgccgt ggtcgtcgcc acccacgacc
     4441 cggtcctgat cgaggcagcc gatcacgtcg ctcgcctggg cgaggagctc ccgccgcccg
     4501 cggcaaccgg acgggtggcg gctcccgtcg cacccgacgt cgcggagccc accacacccc
     4561 agggggatcc gacgcggagc cgatcctctc gaccgtcgcc tccgaacggt caggtcgtcg
     4621 atcaggtcgt tgatcaccac gccggacaca acgccgagct gacccgtccg gaggacgatc
     4681 tcatctggtt ccgtcggatg tcccgaccgg tgcggtggcg gctccgcagc ggtcgtgccc
     4741 tggccgtgtc gaccgacctg tgcggcatcg cactggccgc caccgcggca tggatgctcg
     4801 cgaaggcgtc gatggggacc gcgttcgccg agctggcggt cgccgcggtc ctcatccggg
     4861 tcttcgccat cggcaagggc ctcctgcgct actcggagcg actggtctcc cacgacgcca
     4921 ccttccggat gctcgccgac gtcaggggcg cggtcgtggc ccggctgggg cggatcgcgc
     4981 ccgccggcgt cccgggcatg gagcggggcg acgtgatggc gcgcgtcgtc gatgacgtcg
     5041 accgcctggc cgaccaggag ctcagggtca cggggccgat gacgtcgggg gtgatcgtgg
     5101 ggatggtcgc cgtcgccgcc gcggccgtgg tcgcgccggg cttcggcgtg gtgtacgccg
     5161 cggcggtggt gttgctcgcg atcgtgcttc ccgccgcgac ccggaggagc accgccggag
     5221 cggtgcgcga ccagttggcg caacgaaccc gactggcagg ggcggcactc gaactggtcg
     5281 agcacagcga cgagctgacg gcgacgggcg cgtccgacct gtggagcgcc cgcatcggcg
     5341 acgcggtgca atccctcgga gcggcggagc ggcgtcgcgg tcggcggatc ggtgtgctcg
     5401 gcgggctggc ggctctcgcc gctccatctg tggcggcagc ggtcgtcctc gtcgatggga
     5461 ccgcgaacgg gaccgtcgac ggtgctctgc tcgctgcggt ggtcctggct cccttcgccc
     5521 tggtcgaggt gctggttccg ctgctccacg cgggtgagca gcagccctcg atcgaggttg
     5581 ctgccacacg gctgcgagcg gtgctcgacg ctcccgatcc ggtcgtcgac ccggaggacc
     5641 ccttggcggt tccggcccac ccgtcggtgg tgctcgacgc cgtgtcgctc cgctggccga
     5701 acaccaccga ccccgtactg cggggtctcg ccctgcactt ggaccccggt tcccgcgccg
     5761 agatcaccgg gcccagcggc accggcaagt cgaccctggc ggcagccatg gtccgcttca
     5821 tcggagtgga cggcggccgc tacctcctcg atgagatcga cacagcggac gccgagggcg
     5881 acgacgtccg ttccgtggtg acgtggtgcc agcagtcgcc gtggctcgcg tcgacctccc
     5941 tgcgcgagaa cctgcgcatc gccgcacccg acgccggcga cgacgagttg tgggccgcgc
     6001 tcgatgcagt gcacctgtcg gactgggcgg tcgcccttcc cggcgggctg tcgagcgaag
     6061 tcggccggga cggcgacgcg atgagcggtg ggcaacgcca gcgtctcgcc ctggcccggg
     6121 tgctgctggc cggccactcg atcgtcgtgt tggacgagcc gaccgcgcac ctcgacggac
     6181 cgaccgccga acgggtgcgc gccgatctgc tggcggctct cgatggtcgg accgtcatcc
     6241 tcctcggcca cgaccgggcg agcggcgacg acggtcccac cgccatcgac ggccggtgga
     6301 tgcacgtcga cctctcccgc tccagttccg tgtgaagccg gcgttgccct gccacggtgt
     6361 gggcacgccg cattccgccc cggggctcag cgctgccatc ccggtcccgc agcccgttgc
     6421 cagctccggt gctcccgggc gatcgcgttc gcctgcgaga gcaccgcgtc gcgggcgatg
     6481 cgagccgagg ctcccatcgt ccggccccga aggacgtgga tgccgtcgtg ggtcgcctgg
     6541 cgatggcccg cggcgcggaa caggagccct cgaccggggc actcctggcg ggcgagcgag
     6601 accggtacga gcgtgacgct ccgttggtcg gcaaccagtt cgaggcacgc cgcggtggat
     6661 tcgacctcgt agaacggcag ccgggtcgcc cggtgttccg gatcgaggga ccgcgtcagg
     6721 acgcgtcggc cgacgggggc aggaggcggc agcacgaacg ggtgggccgt cacctgtgcg
     6781 ggagacggtt cggggatccc cgcgagcgga tgatcggcac cgacgacgag cgcgagcggt
     6841 tcggccggca gcggctccga gtcgatctca gcggcgaacg aggactcctc cgccttggag
     6901 gtgtcgcaca cgacgaagtc gaggtcacgg cgtacgagcg acgcgagcag gtccgaggca
     6961 ccgccgccga cgacggccac ctggagtccc ggccgttccc tcgacagcgc ggtcacggcc
     7021 cgggtcatca gggggcgggc cggatacggg cccgtgccga tggcgaccga gccgccctcg
     7081 ccgtctcgca ggagccgggc cgactccacc agcgtgcgag ccgcggcgac ggcgtcgacc
     7141 gcgtggccga tgagcgcctc tgccgcatcg gtcggctcca ccgcctgggt gctgcgatcg
     7201 aacagcgtct ggcccaggtc ggcctcgagg gcctggatgc tgcggctgag cgccgactgg
     7261 ctcaggtaga gctcgcgggc tgcacgggtg aagctccgca accggtacac ggcctcgaag
     7321 tgctcgagtt ggcggagttg caccgacctc acccgccccg acccggatgg atggtgctgg
     7381 tgcgggacat ccgctgccca tgagcatcac tcattacctg atgcatgtcc atgaggttac
     7441 ggtcttcttc gatgacttcg tcgacaccac cggtccccgg ctcgatcgat ctgcgcgacc
     7501 gatcccgggc attcgccgac ctcgaccggg agaccttcga cgtcgtcgtg atcggaggcg
     7561 gcatcaccgg ggccggcatc gcccgcgacg ctgccctccg cggtctgcgg gtcgctctgg
     7621 tcgaggcccg ggacatcgcg tcgggcacgt cgagtcgcag ctcgaagatg atccacggcg
     7681 ggctccgcta cctggcccag ggcgacgtcg gcctggttcg ggaggcagcc accgaacgtc
     7741 aggtgctgcg acgcatcgct ccacagctga cccgcctgtc gccgttcgtc atgcccggca
     7801 gccggtccat gatcgccaag gtccgcgccg ggctggtcgc cttcgaacgc ctgggcaagg
     7861 tcccccgcga cgagcggcac cgcaccctcg acgcgaccga gctcctcgaa ctggagccga
     7921 ccctggctgc gaaccggttc tcctccggtg tcgtgtaccc ggagttcctc acaaacgacg
     7981 cccggttgac cgtcgccaac gtccgctccg cggtggcgtc cggtgccacg gtcctcaccc
     8041 acgccgccgc tacgcgcatc accgtcggca gcaacggccg ggccgacggc gtcgaggtgg
     8101 ccggaacgct tcccggcgag gatctcgcgg cgagggcccg cacccggctg gtggtcaacg
     8161 ccgccggccc gtgggtcgac gcggtgcgtg ccctcgaggc gcctgacgaa cccgcccgcc
     8221 tcgcgctgac gaaggggatc cacgtcgtcg tcccgcaccg acgcctgccc gtccgcgaca
     8281 ccgtcatcac gctcggaccc gacaagcgtc ccgtgttcgc cgtcgccgca ggtccggtca
     8341 cctacctggg cacgacggac accttctaca cggagcccga cgagtggccg cgcatcgatg
     8401 cctccgacgt gcagtacctc ctcgacgcca tggaacagac cttcgacggg cccccgatct
     8461 ctcccgacga cgtcgtcagc ctctggtcgg gggtccgacc gctgatcgcc ccgcccgaag
     8521 gaagcgacgc cgctccctcg gagctgtccc gacgcgacga gacctgggtc ggaccggccg
     8581 gggtggtgtc ggtggccggg gggaagctca ccgcctaccg aacgatggcc gagcgcatcg
     8641 tcgatgatct ggtcggcgat ctcggcaccg gttcactccg gtgccgcacc gcggacgtgc
     8701 ctctgatcgg tggggacgtc gatgtcggtg atctcaccgc agcactcggc agcagcctcg
     8761 gcgaggacgc ggcacggctg gtccaactgt ggggatccga cgcatcatcc atatccgatg
     8821 acggcggcga cgtcgccgcc gaggtgcgct tcgccgtgtg ccacgagggc gctctgcgac
     8881 tcgaggacgt gtggatccgc cgcgcggcac ggtcgtggtt caccctcgag ccggtgggca
     8941 cggacctgga cgtggccgcc gcgaccatgg cggacctgct cggctggacc ccggagcgac
     9001 aggccgctga ggtcgcggca tgccacacga tccaccacga cgcccgccga tgcctcgacg
     9061 agctcgctga cggcgccccc accccggtcg cctgagccgc gcgaggctca ggtccgcacg
     9121 cccgccatcc gacctgccgc ctccccatcg acccacgact ggacgaccac catgctcgac
     9181 tcgaccatcg acaccctccg agacctgctc ggcaccgacg tcgtctccac cgacgccgag
     9241 gtgctcgcca gccgacgccg cgactactgg gcggccagcg cgctccgcga gtggcggggc
     9301 gacacgatgg gtgagcccgg ctgcgtcgtc cggccccggg gcccggagca ggtccaggat
     9361 ctcgtgcgct tctgcggtgc caaccgcatc gccctcatgc cgttcggtct gggctccggg
     9421 gtgtgcggcg gggtcctgcc caccgacgac gtggtcctcc tcgacatgtc gtcgatgtcg
     9481 cggacccgcg agatcgacga gcacgacctt ctcgccacct tcgacgcggg caagaacggt
     9541 ctcgaggccg aggaggaggt cgccgagcgt ggcctcacga tcggacactg gccccagtcg
     9601 atcgcggtca gcagcgtcgg cgggtgggtg tcgacccggg cgtcggggca gttctctacc
     9661 gcctacggca acgtcgagga cctcgtgtac tcgatcgagg cggtgctccc cgacggcgag
     9721 ctggtccacc tcggcaaggc tccgcgtgcc gccgccggcc ccgacctccg acacgtcgtc
     9781 atgggtgcgg agggcacgat gggagtggtc accggcgtga ccttctcgct gcgccgcgcc
     9841 cccgagtcgc gacggctcac cgccttccac tcccccggcg ccgccccgac cgactcgatg
     9901 gctctcggca tcgaggtcca gcgtcggatc gtccaatccg actggcgtcc cccggtgatg
     9961 cgcctctacg acggctccga ggttgcccgg aacttccccg agcacagatc cggcgacgac
    10021 gccgctctcc tgatggtcca cgagggtccc gccggccggg tcgacgccga ggtcgcggac
    10081 gtgacccgga tcgccaccga ggccgggctg accgtggccg acgcgggcat ggtcgagcac
    10141 tggctcgagt cacgcaacca cgtcccgacc tggacggact tcttcgagcg gggtgcggtg
    10201 gtcgacacga tcgagatctc cgcgccgtgg tcgcgcatcg tcgacgtcta cgccgcggtg
    10261 gtcgcctcgc tcggccaggt cgagggaatc gtgaacgcct ccgcgcacag ctcccacgtg
    10321 taccgctcgg gcatcaatct gtacttctcg ttcgcggtgc tcgccgaggt ggccgacatg
    10381 gaggaccgct acttcgagtg ctggcaccgg accatggagg ccacggccgc gaacgggggt
    10441 gggatcgccc accaccacgg atcgggccgg ctgcggatcc cctacctgca ccacgacctg
    10501 ggcgccggcg gcgtgcaact cctgcggacc ctgaagggtg cggtcgaccc cgccggcatc
    10561 atgaaccccg gcaacctcat cccctcacca ccccgctgac ccaccccggt cgacggtccc
    10621 acagcgttct gtagtgcgag atccaggcga acgcctggat ctcgcactac aaagcggcca
    10681 ggagggcggc gatggcggtc gtccagctcc gctcgaccgc ggggcgtgac accgcccggg
    10741 caccgacgag gaggtcccag gcctcgaacg acgccaccgc atcgatcgcc gcggcccgcg
    10801 acgggacctc cgcgggatcg acccggtcca gctccggtcg gaacacgatc agcacctgac
    10861 gtagccagcg ggcccgggct cgaccgaggg cccgggcgat gtcggcgtgg tcgatcgccc
    10921 gcgcccgggc catgcgaccg acccccacgg tcacctcgta cacgtcgagt cgaccggcca
    10981 cgaaccgctc gatcctctcc gaggtcgaca ggtcgctgag gtcctccatc cgggccaggg
    11041 cggtggcccg ggcgatctgc tcgtcgaacg cggcgcggta cagctcgtcg agagtgcgga
    11101 agtaccggaa cagcgacgcg gccgacactc cggcacggcg gcacaccacg tcggccgacg
    11161 ggcgcagctc cccggtgtcg aagagctcga ccacggcctg caccgcggcc gcacgtcccc
    11221 gctccctccg ggccgagcga ccgtcgaccg ggccatcgcc aggtcctccg agcggagcct
    11281 cggttcccga gtccggcggg tcggacagtt gtgagagcat cactctcaca ttatcgctat
    11341 catcgcgtcc cggtgccgac cggacgtccg gaccggacgt gtcgacccca tcgcggcgcc
    11401 agggggaacc atgcggacag agcgtttcga gggcacgatc ggggcgacgt tggccgagtc
    11461 cacgcccgac ttcgacaccc cgcccgaccc ggggccaggt gcgcccgacg tgctcgtgat
    11521 cgtcctcgac gacaccggct tcgcccaact gggatgcttc ggatccgaca tcgacacccc
    11581 gaacatcgac gccctggccg cggacggcgt gatgttcacc aacttccacg tcactcccct
    11641 gtgctccccc acccgggctg cgctgctcac cggccgcagc caccacgacg ccggcatgcg
    11701 cggcctgtcg aacttccgca ccgggttccc ccacatgctc ggccacctct cacacggcac
    11761 cgcgaccatg gccgaggtcc tctccgatgc cggctgggcc accttcgccg tcggcaagtg
    11821 gcacctggcg cccatggagg agtgctcggc cgccggaccg ttcgaccagt ggcccctcgg
    11881 gcgaggtttc gaccgcttct acggattcct cgacggcgag accgaccagt tccacccgtc
    11941 gctcaccgtc gacaaccacc acatcgaccc gcccgcgaca cccgaggacg gctaccacct
    12001 gagcgaggac ctggtcgacc gggccctcga gatgatcacc gcctccacca acatcagacc
    12061 cgaccggccg gtcttcacgt ggttcgcact cggggccacc cacgcccccc accaggcccc
    12121 cgcgccctac cgcgacaagt accgggggcg ctacgacgag ggttgggacg tcgtgcgtga
    12181 acgttggttc gagcgccaga aggagctcag ggtcgtcccg ccccacaccg agctgtcgga
    12241 tcgcaacccg ggcgtcgagg cgtgggagtc cctccccgac accgagcgac ggctggcggc
    12301 acggctgcag gaggcgttcg ccgcgttcct cgaccacacc gatgaccaga tcgggcgtct
    12361 cctcgacggg ctgcgcgacc tcggccgcct cgacaacacc atcgtcgtgc tcgtctccga
    12421 caacggggcc agccaggagg gcggcccgta cggcgtcatg cacgagatga agttcttcaa
    12481 cggcatcctc gagacgccgg agcaggcgat cggccgcatc gacgacatcg gcggccccca
    12541 cagccacacg aactacccgt ggggctgggc gcagtgcggc aactcgccgt tccgctggta
    12601 caagcagaac acccacgaag gcggggtcca cgtcccgttc atcctgcgct ggccccgggg
    12661 tctggccgac caggccggca cgacccgccg ccagttcgcc cacgcctcgg atctggcacc
    12721 caccctctac gagctgtgcg gggtcgacgc tcccgagacg taccgtggcg tcgagcagcg
    12781 gccgatcacc ggtcgttcct tcgctccggt tctccgcgac gccgcctcac ccgcgaccaa
    12841 caccgtccag tacttcgaga tggccggcag ccgggcgatc gtccgcggcg gacccgacgg
    12901 ggagctgacc aaggcggtct gcaagcacga caagggcgcc gactacgaca ccgaaccctg
    12961 ggagctctac gacctgctcg ccgatccggc cgagggccgc gacctggccg ccgagcgccc
    13021 ggagctgttg ggcgagttgg tcgagctgtg gtggtccgag gcccgggcgc accgggtcct
    13081 gcctctcgac gaccgactga tcgagctgtt cggggtgagg ttccggccgc tgtccccgca
    13141 tcccgagagt cgacgctacg tctaccgccc gtcgccgtgg ccgatgccgg cccaggccgg
    13201 cgcagcgatc ggagggcgcg acttcgacat gaccgcgacg gtcgttcgcg aggccggcca
    13261 ggacggggtc ctgttcgcga ccgggaccga gaactcgggc ctcagcctct tcgtgcagga
    13321 cgaccgactg gtgctcgact acaacgcgtt cgacgaccac accgtcgtcg tgtccgatgc
    13381 gccggtcccg gccggggcga gcgtcgtcga actgcgggtg cggcgagtcg accacagctc
    13441 cggcaccgcg ggcctgctga tcgacgggtc gccgagcggg tccgccgagt tgccgctgct
    13501 catgcgcatc atgagctcgg tcggcgccag catcggccac gaccacggct ccgcggtctc
    13561 accgcgctac caggcgccgt tcccgttcca ggggaccctc accgaactgg tcatccagct
    13621 cagccccgac cgcttcggcg gcgacagcga cccgacgacc gcggccgaag cggcccacga
    13681 gatgtcgcgg cagtagtcac cgacccgacc gcaccaccga cccgacccgt ccgagcacag
    13741 gagacgccca gtgaacatcg tccgcacccc cgacagccgc ttcgaggccc tgcccgactt
    13801 cccgttcgag ccgcactacg ccgaggtgcc gacgctcgac cccgaggacg ccgacggcac
    13861 caccctgcgg gtcgcctacc tcgacgaggg cccccgcgac gccgcccccg tcctgctcat
    13921 gcacggtgag ccgtcctggt cgttcctgta ccgccacgtg atcccagggc tcgtcgcccg
    13981 cggccaccgg gtgatcgccc cggacctggt gggcttcggt cgctccgaca agcccaccgc
    14041 cccctccgac tacacctacg cccgccacgt cgcctggatg gaggacctga tcgtcgccca
    14101 gctcgacgtg cgtgacgcca ccttcttcgg acaggactgg ggcggtctgg tcggcctgcg
    14161 ggtcgtcgcc gcccacgccg agcgcttcgc ccgcctggtg gtggcgaaca ccggccttcc
    14221 cgagggtacc ggccgcatca ccgaggcgtt cctggcctgg cagaagttct cccaggagtc
    14281 cgaggacttc ccggtcggag cgatcatcag cggcgggtgc gtcgatcggc tcgcacccga
    14341 ggtcgtcgcc gcctacgacg ccccgtaccc cgacgactcc tacaaggcgg gggctcggat
    14401 cttcccgacg ttggtcccga ccacccccga cgatcccggg ggcctcgcca accaggcggc
    14461 gtggaaggtg ctcgaaacgt tcgaccgtcc agtgctgtgc gccttcagcg acagcgaccc
    14521 gatcaccaag gggggagcca agccgttcct cgagcggatc cccggagccc ggggccgctc
    14581 ccacaccacg atcgagggcg gcggccactt cctgcaggag gaccgcggcc ccgagctggc
    14641 cacggtgatc gcggacttca tcgacgagac ccgctgagac caggcctctg cgggggcgca
    14701 gtgccgattc ctcgatccga tcggtgtcct gccgacggga gggaatggac tcagctccca
    14761 cctcgacctc caccgccaca tccaccgtga ccgacccgga ggccgaggtc cgtcgcgacg
    14821 ctgctcacct cctcggtctg agcgagtggg accccgccgc ggtcgacccc gggatcctga
    14881 ccacggtcac cagcgaacgc aacgggaccg ccgcgaccgc ggcgcgcatg cggcgctaca
    14941 tccggcacct gctggaccac ccggtcagcc acgtgccgga ccgctcggag ctgtggcgga
    15001 ccatgctcga gcactgcgag cgactcacgc cggtgcaggc gtacacactg cgcaacctca
    15061 tgggcccgac gtccagcccg gccggcgacg gcggctacgc cccgatgcca cccgaggtgc
    15121 acctggagtt cccccgcgac gacgccgtcg acctgggcgc ccagtgcggg tggcacttcc
    15181 tggtcggctc gctgtgggac gcggagggca acgagtacgg catcgagttc atgctcttcg
    15241 aacaggcgat gtacccaccg gcgttcgccc gcgagctcgg gctgtcgccg ctcgacaacc
    15301 tcgtggccga ggtgcagttc gccatcagca cccggggcga ccgccaccac caggccgaac
    15361 cactggtcac cctcggcacc agcggcctga tccgaaccgg cacctcaccg ttctcgttcc
    15421 gggtcggggc caactccttc gagtcgctcg aacgggactc cctgatgccg atgcgtgtcc
    15481 gctccgtcgg cgtcgatctc ggcggggaac aaccgctgcc gctgtcgtgc gacctggtgc
    15541 tgaccggggg caaggccgtc ctcgaacaag gcgaccacgg cgcgatgcca tcggtggcgg
    15601 ggatcgggac ctactactac tcgatccccc gaatcgacat cgatccgcag cagagctcga
    15661 tctcgatcga cggacggcag atccccgtcg tggggggcga gctctggttc gaccaccagt
    15721 ggggcttcct ctccggaacc tcccccgacc ccgtcctgcg ggcgtccgac tcgatcaccg
    15781 cccccaaccc cggcggctgg gactggttca tgacacacct gagcggcgac cgccaggtca
    15841 cggtcttcgc cccgcactcc aatgagttcg ccgagttcta cggctgcacc ggagacactc
    15901 cgcccccgac gatgacccgc cgagtcggag gcacgttcat ggacccgaac gccaacaccc
    15961 gcctggtgtg gggaacggtg cacgtcgacc gttgggtcaa ggtcatgcac accccgaacc
    16021 cgcagcgcta cccggcgacg gccacctggt acccgaacca ctaccgattc gagttcgacg
    16081 acctgcccga cgacatctcg gagctgaccc tgacgccgat cgtcgaaggc gggcagtccg
    16141 ccttcttctc ccacggggtg cagatctgcg agggggcggt cgtggtgtcg gaccgcgacg
    16201 gcaacgacat cggtcgggga ttcgcggagg ccgtcgcctt cgccgacacc ctccgcaacc
    16261 ggctgcgcct ggcgggcctg cccgacaccg acgagatggc cgccctggcg gcggagccga
    16321 ccccgtcacc cgagctcgca gccgcgaacg cggcgttcgt cgccgcgcac cccgaccaga
    16381 tggccgccat cgtcgccgcg gcgaagggac tcgagttctt catggagccg tcgtcggagg
    16441 cctgaggttc cccggcgggg cagagccggg tccgcgtggg gcagtctgtc cgtgtggaca
    16501 ccgatgacga cgtcgaacgc aacgtctgcg gcggaccgct gcaggagtgc ggcacggaac
    16561 cgctcaccgg gttcttccgc gacggctgct gtcggaccgg acccgatgac ctgggcagcc
    16621 acacgatctg cgccgtgatg accccggagt tcctggcgca ccagcgttcg atcggcaacg
    16681 acctgtcgac gccgctgccg cagtacgggt tccccggact ggtgccgggc gaccgctggt
    16741 gcgtcaccgc ccgcaactgg ctgcgcgccc accacgacgg cgtcgccgcg ccggtcgtgc
    16801 tgggcgccac ccaccaggcg gtgaccgaac tggtcccgtt cgaggtgctc tgggatcacc
    16861 gcatcgacgt tcccgacgat cccggccctc tgatcgaccc gttctaggtg gccgcgaccg
    16921 tccgaccgga acgcgggacg agccggaccg gtccgttcac caggtgatca cgtcggccgg
    16981 agcgtcctcg aggacctcgt ctcggtcatc ggcgaggagg aagccgtcat cgatcgcccg
    17041 gtcggtcgcc gccgcgtacg cctcccggta cgccgcgaga gtcgggtatc ggtcccggag
    17101 gaccgcatcg ggcagcgcca ggcgccgacc gaacagctgg cacacgtggc tctcgccgtc
    17161 ccgacacagt cccgacagcg tctcgaccgc gacgtcgacg cacggcgtgc ggaccccgcc
    17221 gagcacgttg cccagctcat cggtctcgaa acgccggtgc accaccgggc ccgactcgtc
    17281 gaccgccacc gccaacgggg acgccgaggg aggggcggcg ccggttcgga cccagttcac
    17341 caggtggcgc agggcgctgc gcacgacgaa acgctgctgg ccgcggttga ccggatccgc
    17401 gcaccccagc atcgtctcga gctcaccgat gagcgacagg tcggcatggg cggccccggc
    17461 aagctcccag cagcggaacc ggccggtgtc gggctgccga cacgacaggg agtcgaggaa
    17521 cccgagcacg tcggtctcgg tctgaacgac cagcaccggc acgtccaggt cgtcacggat
    17581 cagaaccgga gggtcacccc ggctcccctc gacgtcggca ccacgcccgg cgtcgcccag
    17641 cggaagcgcg ccgcggcccc ggctgtggat caggaagccg tcgaagcgct ccgcgcgcgg
    17701 ctgcaccccg ttcacgtacg tgaccagggc gtaggccgat tgcgattccc cgaccgcgac
    17761 cacgctctgg atgtcgaggg cagccagggg ttcgggccgg tcgtcgagca gcgagtcaac
    17821 cacctgggtg aacacgtcgt aggcgtaggc atcaccgggg tgcacgaggc cgccgtagcg
    17881 gagcggatcc gcgctctgca gcgcgaggag cccgctcgcc cccacgtcga ccagggcggg
    17941 ggcggccacg atcccggccc actgggccga cacgccgacc cagacgtggc cggcccgggt
    18001 cagctccggt cccgagaagc cgaacaccgg cgccgagtcg gacccgctgc tgacgttgag
    18061 ccactcgacg acgacggtcc cgctgaaccg accctcgtcg ctcggacgcc ggatcaggac
    18121 ccgggtggtg aactcggcat ggtcacgcac ctcgaacgtc cagcgtccgt cggtcgggag
    18181 ccccgacggc gctcgatagg actcggcgtg tcccgaacag gcccactcgg tctgctcgta
    18241 gccgacgtcg cggagcctga cgtcggccag cgaccgggtc agcaccgttc gtcctccgcc
    18301 gatgagcggc tccaaccgtc gtcgtgacgt gtccatcgcc gctcgaccct acggggcgtg
    18361 gacggtccgg tcaccaccgg tcggctggcg gcggggtcga tcccaggaaa caaaccagcc
    18421 ggttggtttc tttgctatgg tggcagccag ccggagaccc ccgacgggtt ggtgcccatg
    18481 gacacgcaga ccgccacacc accgaggacc caggaggatc gccgggccgc gacccagcag
    18541 gcgctgctcg gagccacgat cgactgcctg gtcgagtacg gctacgccgg caccacgaca
    18601 cgactggtgg cagaccacgc ccgggtgtca cgcggggcgc agacgcacca ctacccgacc
    18661 aagcgcgacc tggtcatcgc cgcggtcgag cacctgttcg acgagtacgc cggacgcttc
    18721 accgaggcgt tccgacaggt ccccgagagc gagcgcaccc tcgaccgcgc cgtcgcggcg
    18781 ctgtgggagc tcgcctcggg tcccagctac gcagccatcc tcgaggtcgt cgtcgcggcc
    18841 cgcaccgacg acgagctgcg cagcatcgtc cacggggtgg ccgcgtcgct cgaggagacc
    18901 gtcgtcgacc tgctgctgtg gttctcgccc gagatcgcgg accggggctc agcgcgtcgc
    18961 atcgtcgagt tcgccttcgc cctgatacag ggcgcggcgg tctcccgaat ggaggggttc
    19021 ggtgacgccg acgaggtcgt cgcctcggtc gccgccctgg cccccttcgc cgtgtccctc
    19081 gtccgttccg gacccgaccc gaccagatca ggagctacac gatgacagcc accttgtctc
    19141 ccccacccac caccaccggc cgacgacgcc ggcgtcgggg atctcgcccc tcggccatcc
    19201 gcgacatccc ggtgcgtcgc ctgcgccacg accaggccga ctacgacctc gaccgctggg
    19261 tcgtcccggg ggatccgatc ttcagccact tcctggccat cctctcggcg gtcttcccca
    19321 agggcgagga gttcttcgtc gcgtcggtgc ggcgccaccg cgacgccgtg gcggacaacc
    19381 ccgttctcaa ggagcaggtc aagggcttca tcggccagga ggccatgcac agccgggagc
    19441 accgtcgcct caacgagcgc ctcgccggtc tcggctaccg catggcccga gccgatcgca
    19501 ccatcggcgt gctggtcgac tcgatctaca agatcccgcc gtccacgctt ccgatcgcgg
    19561 cgaccgccgc tgccgagcac tacaccgggc tcctggccga agcggtcctc tcggacgagt
    19621 cgacgcgcac gaccctgttc ggcagcgagg ccgtcgaacc gatgatcgcc tggcacgccc
    19681 tcgaggagct ggagcacaag aacgtcgcgt tcgacgtgat ggaagccgcc ggcggcggct
    19741 acgtcgttcg ggtcgcgggc ttcgcggtca ccacgtcggt gttggccagc tacgtcgcga
    19801 tcgagtgggg ccgcgccgtc tgggaggatc ggcacctgat cacccgggag aaccgccgca
    19861 ccttccacag gaacctgatg cgtcagaagc tcctcagccc gtggtcggcc cgaaaactgc
    19921 tgatctacct ccggcccgac ttccaccccg acgacaccgc caccgacgag ctcgtccagg
    19981 agtggcgcct ccgactcgcc gaggtggcga cggccaacac caaggccgac accgagcccg
    20041 ctcccgccat cgacccggac ccgaccgtct gaacggggtg gtccgtccgg accggcgggg
    20101 agccggacgg accacacact agaacacggt tccagtttgg ggtcgattcc cgtagactgc
    20161 tcgacatggt gcagccgacc ggcccgggaa ccgatcgcga cgactgcgac acctcccggg
    20221 ccgcggagcc gaccaccgac gcgcagcgcg accgacgacg acggatcgtc ctcacggcga
    20281 tgaccatgct gcagcagcgc ggctacgacg agatccagat gcgcgacgtg gcccagcagg
    20341 cgggcgtcgc tctgggcacc ctctaccggt acttcccgtc caaggagcag ctcttcgccc
    20401 acgtcctggt cgagtggagc tccagcttcg acgaggcggt ccgccgtcgg cgccccgaac
    20461 gcgggagcga cgccgagcgg ctccgacagg tgctgcgccg agcggtcggc gccttcgagc
    20521 gccacccgaa cttcctgcag gtgctcacgc tgctggaggt gtccagggac ccggcggtga
    20581 tggagccctt cgccacctac agcgaacggt tcgtctcggt ggtcaccgac gcactcctcg
    20641 acaccgccga ggacgacgtc ccggtgctga tcctgatcac caactcactc ctggtgacgt
    20701 tgctgcaggg ctggtggctc ggtcggtact ccatccggtc ggtccacacc ggcatcgacc
    20761 gggcagtcga catcgtgttc gagggggccc ggaccgaccc cgccgcgccg agcaccgcgt
    20821 gaccgctcgt cagtagcgct gcgggacgaa ggtctgctcg tcgatgggcg gccgttcgta
    20881 ggtcctcacc ggggccggcc gcggcggcag ctcgagcggg tccgggacca cgtcccggta
    20941 ctcgacctgc gtcagcaggt ggctgatgca gttgagtcgc gccgagcgct tgtcctctcc
    21001 gtcgaccacc caccacgggg acccggcggt gtcggtgacc cggaacatct cgtccttggc
    21061 gtgggagtac tcctcccagc ggttccgcga ctcgatgtcc atcggtgaca gcttccaccg
    21121 cttgagcggg ctgtcgatgc gggcctggaa gcgtcgttcc tgctcctcgt cgctgatcga
    21181 gaaccagtac ttgaccagga tgatcccgga gcggatcagc aactcctcga actgcgggac
    21241 cgcccacagg aactcgtcca cctcggcctc cgacgcgaag cccatcaccc gttcgacccc
    21301 ggcgcggttg taccaggagc ggtcgaacag cacgatctcg ccggcggccg gcagttcggc
    21361 gacgtagcgc tggaagtacc actgcgtcct ctcacgatcg ctcggagggc cgagagcgac
    21421 cacccggacc acgcgatggt tggtcgcctc ggcgatgcgg ttgatcgcgc cgcccttgcc
    21481 cgcggcgtcg cgtccctcga acagcaccgc cacccgcagt ccggtggcct tgacccattc
    21541 ctggagcttg accaactcga tctgcaggcg ctccagttca ctcaggtaca cctctcggcg
    21601 gagccgaccg cgcttggtgt agcactcggg cgggaagatc ctctggccgt cgtcgacgct
    21661 catggggtga gtctgctcgc cgtcgccgcc ccgtcagcgg atccgtcgga gtgcgcacca
    21721 cgtgccggcg ccggcggtgg actccacgcc gtcgacctcg aacggcaggg ctgccacgac
    21781 ctcggtcgtg gtcaggtgga tcggggtgtc gggcccgctc acgttgaccc agagcaggat
    21841 gccgccgggg cggagcaccc gggcgatctc cccggggaac aggaacatgt tgatgacgac
    21901 ggccaggtcg accgatccgg agcagagggg cagcgccgag gagtcgccgc ggagacgggc
    21961 cgccggagcg gacggcgcct cgcggagcat ggcgtcggag aggtcgaccg cgacggctga
    22021 acccaccaca cccgcgacca ggtcgctgta gatgccggtc cccgatccga gctcgatccc
    22081 gacggtgtcg gtgcccccgg tccaggccat cgcgcccagc ccccgcccga ggcagtccga
    22141 caccacggca cgacgttggg gcgagtcacg ggtgtgccag tcgggggcga gtccgtcgaa
    22201 cagcgccgcg accgtccggc gtgtctcggc gtcccatcca ccggggacca ggccggcggc
    22261 ccgccgcgtg actgccctca tcgggtgggt gtcgttgccg gtcgaggtgg gcgcgtgccc
    22321 atcgatcgcc actggctcga gagcggtcac cgacatcacg gtgtgatcct cgcgcctggg
    22381 atgcccggcg tcttggaccc agctacttcg atacacgaag tggtttctga cgcgatgtca
    22441 gattctccgc cgccaggccc taccgtgtgg gtcggcccgc acgggcgaac ccgcgaccgg
    22501 gaggcatccc cccatgaccg agtccgactc ccccacccct gacggtccgt ccgatgagga
    22561 cctggacgct gcgctggccg cggcgcggaa gcgctaccag gccgagcggg ccaagcgggt
    22621 ccgttccgac ggcaacgccc agtacgggga gctgaccggc gacttcgccg agttcgaccg
    22681 ggacccgttc gccgatcccg actaccaccg cgatccgctg aacgaagagg tcgaggtgct
    22741 ggtcatcggc tgcggcttcg ccgggatgct caccgccata gagctcaagg cgcgcggctt
    22801 cgacgacgtg cgcatggtgg accgcgccgg cgacttcggt gggacgtggt actggaaccg
    22861 ctatccgggc tgcatgtgcg acgtcgagtc gtacacctac ctccccctgt tggaggagac
    22921 gggctacatg ccgaccgaga agtacgcgag tgcggtcgag atcttctcct acgcccagct
    22981 ggtcggccgc cactacgacc tctaccccca cgccctgttc cacaccgagg tcgtcgaagc
    23041 ggtctgggac gagtcgacgc tgcggtggcg ggtgcggacc aactggggcg acaccctcag
    23101 ctgtcgcttc ctggtgaccg ccgggggcat cctgcacaag gccaagctcc cgggcatcgc
    23161 cgggatctcc cgcttcaagg gtcgggcgtt ccacaccagc cgttggaact acgacgtcac
    23221 cggaggggcc cccggcgtgt tcatggacaa gctggccgac aagcgggtcg cggtgatcgg
    23281 tacaggggcg acagcggtcc aggtcgtccc ccgggtcgcc gaggcggccc aggagctgtt
    23341 cgtgttccag cgcactccct caccggtggg ccagcgcgac aacggaccga ccgacgaggc
    23401 ctggttcgcc tccctcgagc ccggctggca cgccgagcgg gtcaagaact tcaccgaagc
    23461 cgtcaccggc aagaagccgg cggtcgacct ggtcgacgac ggatggaccc acgtcatgtg
    23521 ggacgacacc cagacgaagt ccgacgaccc cgaggtcacc cgccaacagg agcggtccga
    23581 cttcctcacc atggaggaga tccgccagcg ggtcctccgc atcgtcgagg accccgagac
    23641 ggcggccaag ctccagccct ggtacggcaa gcactgcaag cgggtgtgct tccacgacga
    23701 gtacctcccc gcgttcaacc gccccaacgt gcacctggtc gacaccgacg ggcacggggt
    23761 ggacgagatc accgataacg gagtcgtcgc ctgcggagtc gagtacccgg tcgacgtcat
    23821 cgtgttcgcg tcgggcttcg aggtgaccac cgacctggag caccggctcg gcttcgatcc
    23881 cgagggcagg ggcgggatcc gcatgagcga gcgatggagc gatggagcgc actcgctgca
    23941 cggcatcctc tccgccgagt tcccgaacct gctgatgatc agcctggtcc aggccgggtt
    24001 cggcaccaac ttcctgcact tcctgtccaa gtccgccgag cacgtggcct ggatcatcgc
    24061 cacctgcgag gagcgcggga tcgccaccat cgaggccacc cccgaggccg aggaggagtg
    24121 gtacgaactc ctcctcggtg tcgcgatggg catcgccggc tactccaccc gctgcacgcc
    24181 cggctactac aactccgagc agggtcgcga ctcgaagacc gcccggaacc tggtctacac
    24241 cggcagcctg ctggagtacg ccgggttcct ggagcgctgg cggggcgacg gttccatgtc
    24301 aggagcgctc atcaccgaag ggacctgagc agcgttcctt cggtcggatc ggcggtgccg
    24361 gggaccggag atcagacgac gtagtcctcg ccggtggcgc ggacggtgac gtcggagatc
    24421 accacgcccc agggctggtc gatggcgtgg atgacctcgg ccgcgaccgt ctcgggctcg
    24481 atcgcccaga actccggact gtcgacgtcg accgcggcgc cgcccagttc accggtcaga
    24541 gcggccatga cccgctccat gtaggccggc gccttggccc cggtgatgcc cgtcaccgcc
    24601 tcgggattga tcacacctga gcccagaccg gtgcccagca caccggtcgg ccgcacgacg
    24661 gtcaccttga tccgcccctg cgtctccttg cgcaacgagt ccgacatgac cgcgaccgcc
    24721 gccttggtcg cgctgtacac gcccgatccg gccgttccgg cgttggcgta gatcgacgag
    24781 atgttgacca cgtggccccg cccctgagcg accatctggt cgtaggacgc gcatatccca
    24841 tgcagcaccc ccttgaggtt gatgtcgatg cagcggtccc aggccgcggc cgcggctgca
    24901 tggtcggcga agaacgcgag cggcatgacc cccgcgttgt tgatcatgac gtcgacggca
    24961 ccgaaccgct cgacggcccg cgccaccagc gcctcgacct gacccggatc gcacacgtcg
    25021 accgtcacac cgacggcacg cccggcacgc gcctcgattc cctccgcgac ctgctcggcg
    25081 gccaccccgt cgatgtcggc cacgaccacc gccgcgcccc gctcggccga catctcggcg
    25141 atcaaccgcc cgaaaccgcc accgccgccg gtcacgacca ccacccgtcc gtcgaggtgg
    25201 ctcgctgctc gctcggactg tggcgtcggg ttcgggaccg gggcgtcgtt catcgcggca
    25261 gtctcgcgct cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggcggt
    25381 tcccaccacc gacaggaccg acagcacctt gggcatggcc cggaccaggc cacggccgac
    25441 ccgctgtcgt ccggccgaac gatgctcgac catccgcagg ccgacgtcgt ccatcttcac
    25501 gatgagcgcg acgaccccgt agaccgcggc ggtgatgagc agcgcgacca ccacgagcgc
    25561 ggcggcccgc atgacgagcg actcgtcgat cacctccttg agggcgatca ccatgatctc
    25621 cgccgagagg atgaagtcgg tccgcacggc actcgcgacg accgcgtcct cggcctcctg
    25681 gccccgcagc gcgaccggga cgtcctcggg gtgctcgtcg tgggcgacga gccggtgcca
    25741 caccttgtgg gctccctcgt aggccaggta ggcgccgccg accatcagga tgacctcgac
    25801 caccaacgga gcgatcgcgc tgaggacgag ggctgcgggc aggatgaaga ccagcttgtt
    25861 gcgcaccgaa ccgacggcga tgcggcggat gatcggcagc tcccgttcgg cggccaggcc
    25921 gtcgacgtag gccggagtca ccgccgcgtc gtcgacgacc accccggccg ccttcaccgt
    25981 tgccttgccg gccgcggccc ccacgtcgtc gaccgacgcc gcggccagct tggccagcgc
    26041 ggcgatgtca tccagcagtg cggcgagccc gacggccacg atccggtccc ttctcggcgg
    26101 cgaacgagcc gacagggtac ccggggggcg cgaagtggtc cgcgaccggc tcgatcaggc
    26161 cgacgaccgg gccggagccc accgtgggta gtcgatgccg gccagatggt tgatcacgta
    26221 cccctggagc accagcagac ccacggccag catcagcacc agcagctgga ccggctcggt
    26281 gaggatcccg agcgacacga tcagggtggt ggcacccgcg ggcggatgcg ggactcggag
    26341 ccacaccatc agtccgctgg tcagcccgag cgagagcgcg gccgcgccga cgcggggcca
    26401 tgtcacctcg gtcgccagag cggctcccgc atcggtgagc ccgaacacca ccagggccag
    26461 gtagcccgcg gcggccccga tggcgtggcc gcagatcgtg ttccgaggcg acgccgccgg
    26521 aacggtcggc gtgtagaaga gcaggaacgc cgtgggcccc agcgacggga atatcagcgc
    26581 ttgaccggtg acgaccgcgg cagccgacat gatggcgatc gagatcacac cgttcacgag
    26641 ggcgaacagg cccatcacgt tggtcgagtc gtgtctcgcc gtcagggcgg ggagccggaa
    26701 ccgggtgccc agcccgtaga cgatcgacga gatgtcgccg ccgatgggcc cgggcccccg
    26761 tgggggaccg gtcgcaccgg tcacctctgc cgggtcggtg cccccgggac ggtcgggctg
    26821 gtccggggtg ggctcagtca tggcggacga accaccacag cccccaggaa gacgtcagca
    26881 gcacgatcga gaggatcgcg gcgttgcgag cgagcgccgg atcgtgcgcc gccaccccct
    26941 ccagcgccac gacgagcagg aacgcctgca acgacagcag caggatcagg tacacgagca
    27001 ggccaccgtg acggcgacgg tcggcgcggc gggactccct cattcctcgt ccaccccctt
    27061 ggcccacacg accccgtcgc ggacgtcgat gtcgatgcgc gtcagcgggc gttccggtgg
    27121 accggccacg gcgtctccgt tggacgggtc gaacacgccg tcgtggcagg ggcagaagag
    27181 ccggtcccgg tcgggttgga agaacacgac acagcccagg tgcgtgcatc gctgcgagta
    27241 ggcgcgcagc tccccgccct ggaggtgcac caggatcgcc gggtcctcct cggtcggata
    27301 ccggaagagg tactcgctcg actcgggcac ctcggccaga tcgacgatcg gtcgcggttc
    27361 gcccaggttg gagcgctgga ggtcggccca cgccgcggcg ccgaccgtgc cggctgcgaa
    27421 gacccccgac gcagtgagca ggtagcgggt gaagtcccgt cgggcgatgt gctcctcgcc
    27481 gctcgatgtg atcgggaact cgtcgcgcca cagcggtagc tcgctgtcgt cgccgcagcc
    27541 gaggcacggc aggtccgggg cggacccggt tccgttctgc ggtccgtcgg tggtggtcat
    27601 gcccagccct cctcgatccc gaacgggtcg tcc
//
LOCUS       DAOQOD010000019        27077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_580_length_27077_cov_10.336800, whole genome shotgun sequence.
ACCESSION   DAOQOD010000019 DAOQOD010000000
VERSION     DAOQOD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 27077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27077
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_27077_cov_10.336800"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLZ72_03535"
     CDS             complement(<1..1051)
                     /locus_tag="PLZ72_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNA35404.1"
                     /translation="MDLVARTEPGQPVDELPPPGRAHVAFCSPRRSLRGSGEALRIDL
                     PAPWPEHVDAVADTLAGIVHDGSGGAGRGPVAFGALPYDRDTPASLVVPAVIHGRTAD
                     GERWTTRIGDAAAGSDAATTSDVAASSEDGDEAPTPTDPTSVTISSVLSPREWAASVA
                     TAVDRIGRGELTKVVLARELSVRTDQALDAATLLRRFRAASPAAFAYSVDGFVGATPE
                     LLVSRIGDVVRAHPMAGTLPRTGDPDADSRRAGELLGSTKNRWEHQITIDAVHDTLLP
                     WCSYLDAEPEPSVVAAGPVQHLATLVEGRLGQPEPSVLELVAALHPTPAVGGWPRIEA
                     LAHEIIELHRARLAEQ"
     gene            complement(1083..1814)
                     /locus_tag="PLZ72_03540"
     CDS             complement(1083..1814)
                     /locus_tag="PLZ72_03540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNA35405.1"
                     /translation="MASPDSSPADLIPRADQPLPTGAAKTASVRAMFDTIAPRYDLVN
                     RVMTFRMDVGWRRRALDLLGLAPGARVLDLATGTGDLAAEMASRGLVPIGADLSFGML
                     AAAPTPFPRVQADGVALPFPDGALDGATCGFALRNFTDLAATVAELGRVVRPGGRLAL
                     LEVAEPPNPIMRMGHGIYFGRVVPVIGGILSNGRAYSYLPRSVEYLPEPAELLDVVRD
                     SGFRDVDRTLLSGGIAQLITATRVL"
     gene            complement(1848..2465)
                     /locus_tag="PLZ72_03545"
     CDS             complement(1848..2465)
                     /locus_tag="PLZ72_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNA35406.1"
                     /translation="MSIGAPVVAWDLGNVIIPWNRRDALARFVDDEDEIERISREVFD
                     LSTNLHLDRGATVDEVRRIVEERHPGHGWVVDGYVEHFRHSLGPADEATVALIDELLH
                     VGVRCVGLSNWSAITWAGITDAYPALGRLEGVMISGEEGHCKPDEVIFRRCESRFGFT
                     ADQVLFFDDSTANVEGARAVGWDAEVFTTARAARAALVRRGLLSV"
     gene            complement(2462..4198)
                     /locus_tag="PLZ72_03550"
     CDS             complement(2462..4198)
                     /locus_tag="PLZ72_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA35407.1"
                     /translation="MQTSVDGDQTRSDQGFNLATIHETVAAAVGDRDCIVTCRPDGSA
                     ERRSYAQVTERTRRLADALRTRGLGSVVAREQLESHRSGQDHVALYMHNSAEYLESML
                     GAYKARTVPFNVNYRYVADELEYLLADSGARAVVFHSAFAPTLAAVRDRLDRLDVLIQ
                     VPDGSGHGLLPGAEWYEDVLAAATPELADDVVAAWSPDDLYILYTGGTTGMPKGVMWR
                     QDDIFVAAMGGRRPGDQQEWASYEDLAANALNGGVKILPGPPFMHGAAHWVAFNSFTN
                     GNTVVLPSVTERLDVDDLLATVAAEGVNVVLIVGDAFGRPLVEGIEAAVAAGTPDLSS
                     LLAIASGGAALSSGVKSRLLAALPSIIVMDGLGASETGQQGTQLTTAGQEASTGDFTP
                     GPGMCILSEDLDRVLEPGHDELGWLGQSGRVPLGYLGDEAKTARTFPVIDGTRYAVPG
                     DRARLGEDGRLELFGRDSVTINSGGEKIFAEEVEQALLHHPAVRDCVVAGRPSERWGH
                     EVVALVVVDPDAEPVGDDELLTEAARHIARYKLPKAVLRRDALQRSPSGKADYRWAAE
                     QATRLVTDGAGS"
     gene            complement(4281..>5644)
                     /locus_tag="PLZ72_03555"
     CDS             complement(4281..>5644)
                     /locus_tag="PLZ72_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35408.1"
                     /translation="WRPAPLRWASCEDDPNDPDLSCATLVVPLDWSRPDGATIELAVA
                     RRAATGAAGDRIGTIVANPGGPGGSGVELIEDDPFSAGIARRFDRVGWDPRGVGDSTP
                     VTCGTDTVTRFVAADPDPDSPAEQAELERLARAVSDDCATHDAELLAHIGTRDVVRDL
                     EALRLALGDGPIDYVGFSYGTQIGQMYADAFPAGIRTMVLDGVVDPSKGFTEFLMGQI
                     DGFDAAFDAAAARCSARSRACGVEDLTAAYDRVRDRVERAPLRTDAHTPIGHAELATA
                     AIYTGYLTDGWRQLGPALASALDGDGTALLALSNAYHDLSGYGTYAGVVCTDTPPPPD
                     AASWKTFADQARARSPRFGGSVANELLPCATWPVRSDVVPAAVTAPDAPPILVVGNTG
                     DPATPLSGARAVASTLRSAVLVTVDIEGHTAYGRNRCATDLVDEYLISGRTPDAARSC
                     PAD"
     assembly_gap    5645..5744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5745..6157)
                     /locus_tag="PLZ72_03560"
     CDS             complement(<5745..6157)
                     /locus_tag="PLZ72_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNA35409.1"
                     /translation="MATEVNGIAGLKELVGTHLGYSEWMEITQERVNTFADATGDHQW
                     IHVDVERATAESPFGGPIAHGYLTLSLGPVLFPQIMTVKGIKMGVNYGCGKVRFPSPV
                     PVGSNLRLGATLDSVEDLPGGWAQISMTFTFEVEGA"
     gene            complement(6290..6706)
                     /locus_tag="PLZ72_03565"
     CDS             complement(6290..6706)
                     /locus_tag="PLZ72_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35410.1"
                     /translation="MAPLSERRWYNPRLPQTLVISQWLLYFNAFWALLAVFTGGVMGS
                     AIGAVLLLASLGANVYGAYGIANELKLGYQVAVVAAFLPFVLRLVIVFISGASLMRNI
                     GFVLVPGDIINAIFVYALVALLLHPQSREHQKIWFS"
     gene            6705..7535
                     /locus_tag="PLZ72_03570"
     CDS             6705..7535
                     /locus_tag="PLZ72_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA35411.1"
                     /translation="MADSLAEPEPERVVRLAGVVLAAGAGVRLRPLSAWRPKALCPIG
                     ATTLVDRAVAQVAAVTGTPVAVNVHHGRAAMLEHLEDRIDVHVSLEEERALGTAGAVA
                     HLAGFLDGRGALVVNADTVHAEDLRAFVAGWDGRRVRVLATPDPDAGPDGGEGPAFGP
                     RSGIVASIVPWEHVQALVPEPSGLWELLWRPELEAGRLDVVMATGTVIDCGTPEQYLR
                     ANLWVSGGRSVIGAGAVVRGRLERSVVWPGATVEDHEHLIDAVRADPGPGPGGTVLIR
                     "
     gene            complement(7616..8695)
                     /gene="rlmN"
                     /locus_tag="PLZ72_03575"
     CDS             complement(7616..8695)
                     /gene="rlmN"
                     /locus_tag="PLZ72_03575"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNA35412.1"
                     /translation="MSTPGGSRPGPVSRRTRYDLGRDELSELLTGEPAYRVEQVWDGL
                     YRQGRTVSELTNVPKALRERLAAQLPTALDEVTRVTGDDGRTVKFLWALDGGSRIETV
                     LMHYDDRSTVCVSTQAGCAMACGFCATGQAGFERHLSAGEIVEQVVMARREAGDRRIS
                     NVVFMGMGEPLANYDRTWEAVHRLHDDIGIGARHLTLSTVGLVPGIRRLAVADLPVNL
                     AVSLHAADDELRDELVPINRRYPLPVLVEACRDYVDRTRRRLSFEWAMISGVNDRFED
                     AARLADIATDLRAHVNLIPLNSTPGYAVRGSSRNRVQAFAEDLRRRGVNVTVRDTRGS
                     DIDAACGQLRAGHEATAVAPPARRR"
     gene            complement(8692..9408)
                     /gene="mshD"
                     /locus_tag="PLZ72_03580"
     CDS             complement(8692..9408)
                     /gene="mshD"
                     /locus_tag="PLZ72_03580"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HNA35413.1"
                     /translation="MADDASAFERGPSDPRPEPEWVSAVTPDVDAVMRSEGRSMLRLL
                     HQMRVDLPLAGSVLASTRSIDVRAFEPGADDDAWLSVNNRAFRWHPDQGGWDRARLTA
                     RLAEPWVRADGFLVHDGADGDLDGFCWTREHPAGPAPDGTEDPSMGEIFVIATDPSTA
                     GTGLGRALTVAGLDHLTERGLRVGMLYVEAVNTAALRLYERLGFVVHHSNAAYTPARR
                     HDGTDGTAGPATNPDPETTS"
     assembly_gap    9659..9758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9855..10064)
                     /locus_tag="PLZ72_03585"
     CDS             complement(9855..10064)
                     /locus_tag="PLZ72_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35414.1"
                     /translation="MSLHADLASMQSTLDQVLARVDEAASVVRVTDRDDLLGDLYEVE
                     RNLQAAQRRLRRALEAAEHFVEPRA"
     gene            10117..11439
                     /gene="hisD"
                     /locus_tag="PLZ72_03590"
     CDS             10117..11439
                     /gene="hisD"
                     /locus_tag="PLZ72_03590"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNA35415.1"
                     /translation="MLNRLDLRGVPDPSGSLPRPQVDGDEPVAAVRSILAEVRRRGDA
                     ALIELTERFDGVVLERVRVPAEDIDAALAATPDDVRVALELARDRIRAHHETQLRAEV
                     VHDTDGVHIRSFHRPVDRVGCYVPGGRAAYPSTLLMTAVPAQVAGVPRVAVCVPPDRD
                     TGRVTPVTLAAAAVAGVDEVYAIGGAQAIGALAYGTEQVPAVDVICGPGNKYVALAKQ
                     EVAGTVGVAAAFAGPSEVVVIADGTVRPELAAVDVILQAEHGPDGLAWLISWDSDALD
                     AIDVELARLTEAAPRRAEITATLADGGYAVLVDSPAAAVEVANLVAPEHLELLCVGAE
                     DLVAAVRNAGAIFVGPWSPASVGDYVAGPSHVLPTYGTARFSSALTVDDFLKHHHVIT
                     VDGDAFDRIGPAVQSLAEAEGLDAHAESIRIRRDLRDRGIDGHRGATS"
     gene            11436..12530
                     /gene="hisC"
                     /locus_tag="PLZ72_03595"
     CDS             11436..12530
                     /gene="hisC"
                     /locus_tag="PLZ72_03595"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNA35416.1"
                     /translation="MSRPPVRDRIALMDGYHSPQVDVRVRLNTNEAPEPPPAAFVDAL
                     AAGLSGIEWHRYPDRAAVALRSRIADVEGAETPGPIDAANVFAANGSNEVLQSICLAY
                     GGAGRTVATFEPTYALHSHIARVCGSDVVSAAREADFTLDVDVARRLLLDRRPTITFL
                     CSPNNPTGRLEPEETVRAVLETVEEIGGLLVVDEAYGQFSPWSALSLLSEDRPVVVTR
                     TFSKTWSAAALRLGYLIGPSWVVEDLEKVVLPYHLDSMTQLAGVLALDHLDDMQRRVA
                     HLVEERGRLEAALSSLEVDMWPSAANFILFRPRGRDGAEVWQELVDRGVLVRNCASWP
                     GLEGCLRVTLGTVEEDDEFLAALTEVLTGR"
     gene            12610..13209
                     /gene="hisB"
                     /locus_tag="PLZ72_03600"
     CDS             12610..13209
                     /gene="hisB"
                     /locus_tag="PLZ72_03600"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNA35417.1"
                     /translation="MSDTPRTAQAERVTKETTISVTLDVDGDGTTDCRTGLPFFDHML
                     DQLGRHGGMDLTLHADGDLEIDAHHTVEDTGIIVGEALRQALGDKRGVRRFASIRVPL
                     DEAVIEAVLDLSGRPYLHYDIDPPGVKILGDPPFDPQLMEEFWRALVTSAGITLHLTL
                     VRGRNTHHIIEASVKAAARALRDAVRVEGTELPSTKGTL"
     gene            13212..13841
                     /gene="hisH"
                     /locus_tag="PLZ72_03605"
     CDS             13212..13841
                     /gene="hisH"
                     /locus_tag="PLZ72_03605"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNA35418.1"
                     /translation="MEAGPRIAVLDYGIGNLRSAEKALEHVGADAFLTADPDRVERAD
                     AVVLPGVGAFGRCREALSESGLDQVALDAVASGRPFLGICVGMQLLYEESDESPGVTG
                     LGVLSGRVQRLPAGVKHPQMQWNQVDLVSESPLFDGLGERPWMYFVHSFAPEVTGDVI
                     GTCDYGGAVTAAVQHEMVFATQFHPEKSGTAGLALLRNFVRLAATATVS"
     gene            13887..14621
                     /gene="hisA"
                     /locus_tag="PLZ72_03610"
     CDS             13887..14621
                     /gene="hisA"
                     /locus_tag="PLZ72_03610"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNA35419.1"
                     /translation="MDLYPAIDLLDGRCVRLYQGDYDRSTVYGDDPVAQALAFQSEGA
                     PWVHVVDLDAARTGEPRNRDVVAAIAGALDVPVQTGGGVRTVEAAEALFSAGVARVVL
                     GTAALEDPDLVRRLAADHPVAVGLDARGSEVAVRGWLEGSGADVLDLAVAYQDAGVAA
                     LVVTEISRDGTLEGPDLAGLAAVVAATKVPVIASGGIGTLADIEALVALAAGGRSLAG
                     AITGRAVYEGAFTVAQAVAVTRGSAT"
     gene            14618..15388
                     /gene="hisF"
                     /locus_tag="PLZ72_03615"
     CDS             14618..15388
                     /gene="hisF"
                     /locus_tag="PLZ72_03615"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNA35420.1"
                     /translation="MTAVARVIPCLDVDAGRVVKGVNFVALRDAGDPVELAARYDAEG
                     ADELVFLDITASSDARETTIDMVRHTAEEVFIPFTVGGGIRSVEDARRILRAGADKVG
                     VNTAAVDRPELVAEIADEFGAQCVVVAIDSRAGDGNGPRFEVHTHGGRTPTGLDTVEW
                     AARVAELGAGEILLTSMDRDGTKDGFDVPVTAAVSDAVNVPVIASGGVGRVEHMVEGI
                     TVAGADAVLAASIFHFGQHTVAEVKAALAAAGVPVRPV"
     gene            15425..16363
                     /locus_tag="PLZ72_03620"
     CDS             15425..16363
                     /locus_tag="PLZ72_03620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA35421.1"
                     /translation="MELRVFTEPQQGATYDQLLAVARAAEELGFGGFFRSDHYLAMGD
                     TAGLPGPTDAWTTLAGLARDTATIRLGTLVTAGTFRLPGPLAITVATVDAMSGGRVEL
                     GLGAGWFDDEHTAYGIPFPPLGERFDRLTEQLEIVDGLWSTPAGKTFDHDGRHYQIAG
                     SPALPKPVQRPRPPIIVGGFGARRTPALVARFADEYNVPFADADTVAALNRGVDEVCR
                     SHDRVPATVLRSAAQVLCVGRDEPEIARRAGAIGREVDELRTNGLAGTPDEVIAKIER
                     FASIGMTRLYLQVLDLDDLEHLALVAEKVMPAVASS"
     gene            16474..16917
                     /locus_tag="PLZ72_03625"
     CDS             16474..16917
                     /locus_tag="PLZ72_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNA35422.1"
                     /translation="MTDADPTDGYPADADPSDGTGPDGAGADGTAAASPSPPRPTGSH
                     EKLPVRMLNDRILVKVDAEGDRRSTGGILIPATAQMGKRLAWAEVRATGPNVRAVEDG
                     DHVLFNPEEVYEVEVRGDTFIILRERDIHAVASERLEAGHTGLYL"
     gene            17028..17450
                     /gene="hisI"
                     /locus_tag="PLZ72_03630"
     CDS             17028..17450
                     /gene="hisI"
                     /locus_tag="PLZ72_03630"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNA35423.1"
                     /translation="MCPAPAPSSDTPPAVTPVAVRDADLELIRYDSDGLVPAIVQDVE
                     TRAVLMMAWMNAETLRMSLEQGRTVFWSRSRQEVWRKGDTSGDRQFIRSAHYDCDGDA
                     LLFLVEQEGKGACHTGAYTCWFRTFGSDEQTTDRPGPV"
     gene            17478..19100
                     /locus_tag="PLZ72_03635"
     CDS             17478..19100
                     /locus_tag="PLZ72_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNA35424.1"
                     /translation="MLTRDQFHRLARDHRVVPVTRTLLADLTTPVAAFARWCGPDSPA
                     DGSPARAGFLLESVDHGGRWGRWSFVGRDPRLRLVSRSGRVEVDGSLPDGLVGDLPTG
                     DGILAVLEVLLEHLRAPTPDALAAVVGTSDAADGPRGWELPPLHSGVMGYLGYDVVRE
                     VEHLPAVPADDRGWPDAVLSVIGEIAAYDHWSQQVTLVANAFVADGADRSAVDLAYDD
                     AVARLERMAADGATPIDEPLVEPPTRADESVAVTSSMSGGTYEAAVDAAKEHILAGDI
                     FQVVLAQRFDFTTTAEPLDIYRTLRQVNPSPYMFLMREDDLTLVGCSPEPMVQLLDGR
                     VISRPIAGTRFRGRTDEHDRRLAAELVEHPKERAEHIMLVDLARNDVGRVVEFGTCQV
                     DELMTLERYSHVMHLTSQVSGALAEGCGPIDVLRATLPAGTVSGAPKVRAMEIIDELE
                     PVKRGPYAGIVGYVDFSGNIDTAIAIRTMLVDPPGADGTRRACVQAGAGVVADSVAAD
                     EELETRNKARALLSAVPAAERMTAYRRAAGGR"
     gene            19100..20047
                     /locus_tag="PLZ72_03640"
     CDS             19100..20047
                     /locus_tag="PLZ72_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNA35425.1"
                     /translation="MATPAAPDEGVVAGVGAARSDVVAVALERDGLVVTGSDAVSFVQ
                     GQVSADVSSLAVGESTWSLLLEPQGKVAAWLRITRTADAELFVDVDAGWGESTLARLR
                     RFLLRVDVVVEPVRRRGLALVGPRAADVADGASGVELSLAVDRRGLAGVDLFGPVDGP
                     APEPPDGVQRVGPEVLEVLRIEQGWPVMGRELDASTIPAEAGAWLIDTSVSFTKGCYT
                     GQELVARVHHRGDNVPRHLRGLVIDGAVLPSPGAPVIAEGVERGAVTSAVVSPTLGGV
                     PVALAMVHRRVELGADVEFDGATARVVDLPFVAPPPPNL"
     gene            complement(20135..21571)
                     /locus_tag="PLZ72_03645"
     CDS             complement(20135..21571)
                     /locus_tag="PLZ72_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35426.1"
                     /translation="MRRRVSAAVIVSVVVALALAGLGTLVLSRAAARRSAEADLRRQA
                     GVVADLAAALGTANQPDAEGNPRLSVTSLRRMSDAFDLHDIGAVIVGPEGRSRGELPA
                     GVTLTPTEEEAVRAGGLVSGESAGGRLYAATGRSVGPAVIVVGMTGTTRGLLSGPAGW
                     FVISSALVVALAVGVGLWLGSRLARPVRDATAVTERIAAGDLDARLPDPFPGAGDETA
                     VLARSINAMADALARSRGLEQQFLMSVSHDLRTPLTNIRGYAEAIADGATAPGDGARV
                     ISRESDRLERLVRDLLDLARLDARQFTLHPRAVDVGSVVADAAESHRAELAAAGLSLV
                     VQADAGLTGTVDPDRLAQVVGNLVGNATRFAAGTVTVTASGEPAESAESAGDPSATTG
                     HRSVVVHVINDGTPIDAADLPFVFERLYQGRRRPTRSESGSGLGLAIVRELVTAMGGT
                     VGVHAPADGGTAFWFRVPLTPVAPPSNL"
     gene            complement(21600..22277)
                     /locus_tag="PLZ72_03650"
     CDS             complement(21600..22277)
                     /locus_tag="PLZ72_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35427.1"
                     /translation="MATIVAVEDDPAIADLLDMYLRREDHRVYLAADGERGLELIAAH
                     RPDVVILDVGLPGIDGLEVCRRIRASDAGVSVVFLTARDDEIDRVLGLEMGADDYVTK
                     PFSPRELVARVRAQLRRAAATAAATTVAPAVIEVGSVEVDLGRHEARRDGEPVGLTTQ
                     EFELLAFLGANRGLALSRRQILDGAWGDGWIGDERTVDVHVGQLRRKLGDDLPLVTVW
                     GVGYRLD"
     gene            22361..23158
                     /locus_tag="PLZ72_03655"
     CDS             22361..23158
                     /locus_tag="PLZ72_03655"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNA35428.1"
                     /translation="MATYLDRILDRHRADAAADERRTEDLVQAAGELPPTRGFRSALL
                     AGTELAVISEVKRRSPSKGDLFADLDPAALAADYQAAGASCLSVLTDGESFGGSADDL
                     AAARAAVSVPVLRKDFTVSANDVCDARLMGADCVLLIVAALSDGELGELSALAVQIGL
                     DVLVEVHDEAELERALATTAGDLVGVNQRDLVTFEVDTARAVRVAAAMPAGTVRVAES
                     GVRGRDDALALAAAGYHAVLVGEHLVTAADPGAALAALRVPRGPRGG"
     gene            23203..23868
                     /locus_tag="PLZ72_03660"
     CDS             23203..23868
                     /locus_tag="PLZ72_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNA35429.1"
                     /translation="MFVKICGITNEEDALMATALGADAVGFVFAPSARQVSVGTVRDI
                     VKRLPPEVVTVGVFRDQAPRLVIDTVLEAGLRAVQLHGHESPQDAAEIRPYAHALIVA
                     LAVGDPALAHVDDYGADALLLDSPVPGSGNVFDWALAEGVPVGRRMILAGGLTPDNVA
                     AAVAEVQPWGVDTSTGVQADGDPTRKDPLKVQRFVRSARAAAPRPYHPEGNGPFDWAD
                     AGL"
     gene            23928..25124
                     /gene="trpB"
                     /locus_tag="PLZ72_03665"
     CDS             23928..25124
                     /gene="trpB"
                     /locus_tag="PLZ72_03665"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNA35430.1"
                     /translation="MTEPVSASGEPVMGEPDPTGRFGDHGGRFVPESLMPALIELDAA
                     FRDAWADPAFRSELDELLRSYAGRPSPLTDCHRLSEEVGCRVLLKREDLNHTGSHKIN
                     NVLGQALLARRMGKTRLVAETGAGQHGVATATAAALLGMDCLVYMGEVDMARQQLNVF
                     RMRLLGAEVTPATSGSRTLKDAVNEAMRDWVATVESSHYCLGSVMGPHPYPWMVREFH
                     RVIGVEAAAQCDDLLGGPPDVVVACVGGGSNAAGIFSGFAEVPGVELVGVEPAGGAAI
                     GGGVPGIVHGMHSYLKQDEFGQVLEAESISAGLDYPGVGPEHSHLADVGRARYEPVTD
                     DEVVDAFVLLSRTEGIIPALESAHALAWVSRARAELAGRTVLINLSGRGDKDVAQMMD
                     VLADRL"
     gene            25121..25921
                     /gene="trpA"
                     /locus_tag="PLZ72_03670"
     CDS             25121..25921
                     /gene="trpA"
                     /locus_tag="PLZ72_03670"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNA35431.1"
                     /translation="MSAVALDLEATLRGRRDAGAKTLVPYLTGGLFAGADGDWTDVLR
                     SVASAGADAVEVGIPFSDPVMDGPVIQQASELALASVATPPGILDELAGVDAGVPLAV
                     MTYVNLVYRFGYERFAATARRCGVAAAIVPDLPLEESEPWCAAADGAGIQTVMLAAPT
                     APDERLERIVARARGFVYAVGLMGVTGERAALADSATVIASRLKAITDVPVLVGVGIS
                     SPEQAVEVCEVADGVVVGTALVRRVLETRSPEAAGELIGSFRDALDRG"
     gene            26081..26365
                     /locus_tag="PLZ72_03675"
     CDS             26081..26365
                     /locus_tag="PLZ72_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNA35432.1"
                     /translation="MNKTELVETISQRTDIAKKDVQAVLDTFEDIAGEIVAKGKDTLT
                     IPGFLKFEQTHRAARTGRNPQTGETIQVPASNAAKVSAGAKLKNRAKTGK"
     gene            26484..26942
                     /gene="bcp"
                     /locus_tag="PLZ72_03680"
     CDS             26484..26942
                     /gene="bcp"
                     /locus_tag="PLZ72_03680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNA35433.1"
                     /translation="MTKLEIGDKAPAIGLKDQDGETVRLSSFKGRKVLVFFYPKANTP
                     GCTNQACGLRDVADQVGDTAIVGISPDMPDKQAAFDQKYSLGYPLLSDPEHKVAEAYG
                     VWGEKKLYGRAFMGIIRSAFLIDEKGKVEQAWYKISPKDTPLKLLAALKD"
BASE COUNT         3643 a         9644 c         9885 g         3705 t
ORIGIN      
        1 gttgctccgc caggcgcgcg cgatgcagct cgatgatttc gtgcgccagc gcctcgatgc
       61 ggggccagcc gccgaccgcc ggggtggggt gcagggccgc gaccagctcg agcaccgacg
      121 gctccggctg gccgaggcgg ccctcgacca gcgtcgcgag gtgctggacc gggcccgcgg
      181 cgaccacgga gggctccggc tcggcgtcga ggtaggagca ccacggcagg agcgtgtcgt
      241 ggaccgcgtc gatggtgatc tggtgctccc agcggttctt ggtggatccg agcagctcgc
      301 cggcccggcg tgaatcggca tcggggtcgc cggtgcgcgg cagggtgccc gccatgggat
      361 gagcgcgcac cacgtcgccg atgcgtgaca ccaagagctc gggtgtggcg ccgacgaagc
      421 cgtcgaccga gtaggcgaac gccgccggtg acgcggcacg gaaccgtcgc agcagcgtgg
      481 ccgcgtccag ggcctggtcg gtccgcaccg acagctcacg ggcgagcacg accttggtca
      541 gctcgccgcg cccgatccga tccaccgcgg tggccaccga cgcggcccac tcccgggggc
      601 tgaggacgct gctgatcgtc accgacgtcg gatccgtcgg tgtcggggcc tcgtcgccgt
      661 cctcggagct ggcagcgacg tcggaggtgg ttgcggcgtc ggagcccgca gcggcgtcgc
      721 cgatgcgggt cgtccatcgc tcaccatccg cggtgcggcc gtggatcacc gccggcacca
      781 ccagcgatgc gggggtgtca cggtcatacg gcagcgcccc gaaggcgacc ggtccacggc
      841 cggcgccgcc cgatccgtcg tggacgatcc cggccagggt gtcggcgacg gcgtcgacgt
      901 gctccggcca cggcgccggc aggtcgatcc gcagcgcctc gcccgatccg cggagacttc
      961 ggcgcggcga gcagaaggcg acgtgagcgc ggcccggggg cggcagctcg tcgacgggct
     1021 gtcccggctc ggtgcgggcc acgaggtcca tctcggtcac cggccgtggt ggcggaccgc
     1081 ggtcacagca cccgggtcgc ggtgatcagc tgggcgatgc cgcccgacaa cagggtccgg
     1141 tccacgtcgc ggaacccgct gtcgcggacc acgtcgagca gctcggccgg ttcgggcagg
     1201 tactcgaccg accggggaag gtacgagtag gcccgcccgt tcgagaggat cccgccgatc
     1261 accgggacga cgcggccgaa gtagatcccg tggcccatgc gcatgatcgg gttgggaggt
     1321 tcggcaacct cgagcagggc gagacgtcct ccgggtctca ccacccgacc gagttcggcg
     1381 acggtggcgg ccaggtcggt gaagttgcgc agggcgaacc cgcaggtcgc cccgtcgagc
     1441 gctccgtcgg ggaacggcag cgccaccccg tcggcctgga cgcgggggaa cggggtcgga
     1501 gcggcggcca gcatcccgaa gctgaggtcc gcgccgatcg gcaccagacc gcgggacgcc
     1561 atctcggcgg ccaggtcacc ggtcccggtg gccaggtcga gcacccgggc tccgggcgcc
     1621 agtccaagca ggtccagcgc ccgacgacgc cacccgacgt ccatccggaa cgtcatcacc
     1681 cggttcacca ggtcgtagcg gggtgcgatg gtgtcgaaca tggcccgtac cgacgccgtc
     1741 ttggcggccc cggtcggcag cggctggtcg gcccggggga tcaggtcggc gggggatgag
     1801 tcgggggagg ccatcgccgc cacactacgg ggcgggggcg aaggccctca caccgacagc
     1861 aggccccgtc gcaccagcgc ggcccgggcg gcccgggcgg tggtgaacac ctcggcgtcc
     1921 caacccacag cccgtgcccc ctcgacgttg gcggtgctgt cgtcgaagaa caggacctga
     1981 tcggcggtga agccgaagcg cgattcgcag cggcggaaga tcacctcgtc gggtttgcag
     2041 tgaccttcct cgcccgagat catcaccccc tccagacgtc cgagggccgg gtaggcgtcg
     2101 gtgatgccgg cccaggtgat cgccgaccag ttcgacagcc cgacgcagcg cacgccgacg
     2161 tggagcagct cgtcgatgag ggcgacggtc gcctcgtcgg cggggccgag gctgtggcgg
     2221 aagtgctcca cgtagccgtc gaccacccag ccgtgtccgg ggtggcgctc ctcgacgatg
     2281 cggcggacct cgtcgacggt ggcgccccgg tccaggtgta ggttggtgga caggtcgaac
     2341 acctcgcggg agatgcgttc gatctcgtcc tcgtcgtcga cgaagcgggc caacgcgtcg
     2401 cgacggttcc acgggatgat gacgttgccg aggtcccagg cgacgacggg ggcgccgatg
     2461 ctcacgaccc ggctccgtcg gtcaccagcc gtgtcgcctg ctcggcggcc cagcggtaat
     2521 cggccttgcc agacggcgaa cgttgcagcg cgtcgcgacg cagcaccgct ttgggcagct
     2581 tgtagcgggc gatgtggcgg gccgcctcgg tgagcagttc gtcgtcgccg accggctccg
     2641 cgtccgggtc gaccacgacg agggcgacga cctcgtggcc ccaacgctcc gacggtcggc
     2701 cggcgacgac gcagtcgcgc accgccgggt ggtgcaggag cgcctgttcg acctcctccg
     2761 cgaagatctt ctctcccccg gagttgatcg tgaccgagtc ccgaccgaac agctcgagcc
     2821 ggccgtcctc gcccaggcgg gcccggtcac cgggcacggc gtagcgggtc ccgtcgatga
     2881 cggggaaggt gcgggcggtc ttggcctcgt cgccgaggta gccgagcggc acccggccgc
     2941 tctgacccaa ccaacccagc tcgtcgtgtc cgggctcgag cacccggtcc aggtcctcgg
     3001 agaggatgca catccccggt ccgggggtga agtcgccggt cgacgcctcc tgacccgcgg
     3061 tggtcagctg cgttccctgc tggccggtct ccgatgcccc caaaccgtcc atgacgatga
     3121 tcgagggcag cgcggcgagc agccgcgact tcacgcccga cgacagcgcg gcgccgcccg
     3181 aggcgatggc gagcagcgac gacaggtcgg gggtgccggc ggccaccgcg gcctcgatcc
     3241 cctcgacgag ggggcgtccg aaggcgtcgc cgacgatcag cacgacgttg accccttcgg
     3301 cggcgacggt ggcgagcagg tcgtcgacgt cgagtcgctc ggtgaccgac gggagcacca
     3361 ccgtgttccc gttcgtgaac gagttgaacg ccacccagtg ggcggccccg tgcatgaacg
     3421 ggggcccggg caggatcttg accccgccgt tgagggcgtt ggcggccagg tcctcgtacg
     3481 aggcccactc ctgctggtcg ccgggccgac gcccgcccat cgcggcgacg aagatgtcgt
     3541 cctgacgcca catcacgccc ttgggcatgc cggtcgtgcc gccggtgtag aggatgtaga
     3601 ggtcatccgg cgaccaggcc gccaccacgt cgtcggccag ctcgggggtg gcggcggcga
     3661 gcacgtcctc gtaccactcg gcgccgggga gcagaccgtg gcccgacccg tcgggcacct
     3721 ggatcaagac gtcgagccgg tcgaggcggt cacggacggc ggccagcgtg ggagcgaagg
     3781 ccgagtggaa caccaccgcc cgggccccgg agtcggccag caggtactcc agctcatcgg
     3841 caacgtagcg gtagttgacg ttgaacggga cggtgcgggc cttgtaggcc ccgagcatcg
     3901 actccaggta ctcggcgctg ttgtgcatgt acagcgcgac gtggtcctgt ccggagcggt
     3961 gcgattcgag ctgctcgcgc gccacgaccg atcccaggcc acgggtccgc agggcgtcgg
     4021 cgagtcggcg ggtcctctcg gtcacctggg cgtagctgcg ccgctccgcg ctgccgtcgg
     4081 gacggcaggt gacgatgcag tcgcggtcgc cgacggccgc ggccaccgtc tcgtggatgg
     4141 tggccaggtt gaacccctgg tcgcttctcg tctggtctcc gtcgacgctg gtctgcacca
     4201 caccccccgg tcggtctcgg agtgtcgatc gtacggtcga cgcccgggcg gggcgaaccc
     4261 tggagcgggt tcgggtcgcg tcagtccgcc ggacagctcc tggccgcgtc gggggtgcga
     4321 cccgagatca ggtactcgtc gaccaggtcg gtggcgcagc ggttgcggcc gtaggcggtg
     4381 tgcccctcga tgtcgacggt gacgagcacg gccgagcgca gcgtcgaggc gacggcgcgg
     4441 gcccccgaca acggggtcgc gggatccccg gtgttgccga cgacgaggat cggtggggcg
     4501 tcgggagcgg tgaccgcggc ggggacgacg tcggagcgga ccggccaggt cgcgcacggc
     4561 agcagctcgt tggcgaccga gccgccgaac cggggggagc gggcccgggc ctggtccgcg
     4621 aacgtcttcc acgacgcggc gtccggcggc ggtggcgtgt cggtgcagac gacgccggcg
     4681 taggtgccgt agccgctgag gtcgtggtag gcgttggaca gcgcgaggag agcggtgccg
     4741 tcgccgtcga gcgccgaggc cagcgccggg cccagctgtc gccatccgtc ggtcaggtag
     4801 ccggtgtaga tcgccgcggt ggcgagctcg gcgtggccga tcggtgtgtg ggcgtccgtt
     4861 cgcagtggtg cccgctcgac ccggtcccgg acccggtcgt aggccgcggt caggtcctcg
     4921 acgccgcagg cccgagaccg ggcggagcac cgggcggctg ccgcgtcgaa ggcggcgtcg
     4981 aacccgtcga tctgacccat caggaactcg gtgaagccct tggacgggtc gaccacaccg
     5041 tcgagcacca tcgtgcggat cccggcgggg aacgcgtccg cgtacatctg gccgatctgg
     5101 gtgccgtagg agaacccgac gtagtcgatc ggtccgtcgc cgagcgccag gcggagggcc
     5161 tcgaggtccc ggacgacgtc acgggtgccg atgtgggcga gcagctccgc gtcgtgggtg
     5221 gcgcagtcgt cggacacggc gcgggcgagt cgttccagct cggcctgctc ggcgggggag
     5281 tcggggtcgg ggtcggccgc gacgaaccgg gtgacggtgt cggtcccgca ggtcaccggg
     5341 gtggagtccc cgacgccgcg ggggtcccat ccgacgcggt cgaaccgtcg ggcgatcccc
     5401 gcgctgaagg ggtcgtcctc gatgagctcg acgcccgacc caccgggtcc gcccgggttg
     5461 gcgacgatgg tgccgatccg atctccggcc gctccggtgg cggccctgcg ggcgaccgcc
     5521 agctcgatcg tcgccccgtc cggacgggac cagtcgagcg ggacgaccag ggtggcgcag
     5581 ctcaggtcgg gatcgttcgg gtcgtcctcg cacgacgccc accggagcgg ggcgggacgc
     5641 catcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgccc tcgacctcga
     5761 aggtgaaggt catcgagatc tgcgcccacc cgccgggcag gtcctcgacg gagtcgaggg
     5821 tggcgcccag acggaggttg gagcccaccg gcaccggcga cgggaaccgg accttgccgc
     5881 agccgtagtt gacgcccatc ttgatgccct tgaccgtcat gatctgcggg aagagcaccg
     5941 ggccgagcga cagggtcagg tagccgtggg cgatggggcc gccgaagggc gactcggccg
     6001 tggcccgctc cacgtcgacg tggatccact ggtggtcacc ggtggcgtcc gcgaacgtgt
     6061 tgacccgctc ctgggtgatc tccatccact cggagtagcc cagatgggtc cccacgagct
     6121 ccttgagtcc tgcgattccg ttcacctcgg tggccatggc ggcatcttgt cagcggtcgc
     6181 ggttggcggg ccagcccggc cggagggcac aggggagcca gcccggccgg agggttcagg
     6241 ggagccagcc cggccggagg gcacagggga gccagcccgg ccggagggtt caggagaacc
     6301 agatcttctg gtgctcgcgc gactgggggt gcagcagcag cgcgaccagg gcgtagacga
     6361 agatcgcgtt gatgatgtcg ccggggacga gcacgaagcc gatgttgcgc atcagcgacg
     6421 cgcccgagat gaacacgatc accagccgca gcacgaacgg caggaaggcg gcgacgacgg
     6481 ccacctggta gccgagcttg agctcattgg cgatgccgta ggcgccgtag acgttggcgc
     6541 ccaggctggc gagcagcagc acggcgccga tcgccgagcc catgaccccg ccggtgaaca
     6601 cggcgagcag ggcccagaag gcgttgaagt agagcagcca ctggctgatc accagcgtct
     6661 ggggaaggcg cgggttgtac cagcggcgtt cggagagggg ggccatggct gacagtctgg
     6721 cggagccgga gccggagcgg gtggtgaggc tcgccggggt ggtgctggcc gcgggggccg
     6781 gggtgcgcct gcgtccgctg agcgcgtggc gccccaaggc gctgtgtccc atcggtgcca
     6841 ccaccctggt cgatcgggcc gtggcgcagg tcgcggcggt gacgggaacc ccggtggcgg
     6901 tgaacgtcca ccacgggcgg gccgccatgt tggagcacct cgaggaccgc atcgacgtcc
     6961 acgtgtcgct cgaggaggag cgggcgttgg gcaccgcggg ggcggtcgcg cacctggccg
     7021 gcttcctcga cggtcgtggc gccctggtgg tcaacgccga cacggtgcac gccgaggacc
     7081 tgcgggcgtt cgtcgcgggg tgggacggcc gacgggtccg ggtgctggcc acccccgacc
     7141 cggacgccgg acccgatggt ggggagggac cggccttcgg accgcgcagc gggatcgtgg
     7201 cgtcgatcgt gccgtgggag cacgtgcagg cgctggtgcc ggaaccgtcg gggctgtggg
     7261 agctgctgtg gcgacccgag ctcgaggcgg gtcgcctgga cgtggtgatg gcgaccggga
     7321 ccgtcatcga ctgcgggacc cccgagcagt acctgcgggc caacctgtgg gtcagcggcg
     7381 ggcggtcggt gatcggggcg ggagcggtgg tgcggggccg gctcgagcgg tcggtggtgt
     7441 ggccgggggc gacggtcgag gaccacgagc acctcatcga cgccgtccgc gcggacccgg
     7501 gcccgggccc gggcggcacc gtgctcatcc gctgatatag gggtcctcac tcacaaccct
     7561 tgtcgctttc gcgccctcag aaatctggaa ccccggcgcc ggcgccgacg tcggatcagc
     7621 ggcggcgggc gggcggtgcg accgcggtgg cctcgtggcc ggcccgcagc tggccgcacg
     7681 cggcgtcgat gtcggagccg cgggtgtcgc ggaccgtcac gttcaccccg cgccgtcgca
     7741 ggtcctcggc gaaggcctgc acacggttgc gcgacgagcc gcggaccgcg tagccggggg
     7801 tcgagttaag cgggatcagg ttcacatggg cccgcaggtc ggtcgcgatg tcggccaggc
     7861 gggcggcgtc ctcgaagcgg tcgttgacgc cggaaatcat cgcccattcg aagctgaggc
     7921 gacggcgggt ccggtcgacg tagtcccggc acgcctcgac caggaccggc agcgggtagc
     7981 gacggttgat cggcaccagc tcgtcgcgga gctcgtcgtc ggcggcgtga agcgacacgg
     8041 ccaggttgac cggcaggtca gcgacggcca gtcgtcggat gccgggaacc agacccaccg
     8101 tcgacagggt gaggtgccgg gcgccgatgc cgatgtcgtc gtgcaggcgg tgcacggcct
     8161 cccatgtgcg gtcgtagttg gcgagcggtt cgcccatgcc catgaacacc acgttggaga
     8221 tccgccgatc gcccgcctcg cgtcgggcca tcacgacctg ttcgacgatc tcgccggccg
     8281 agaggtgccg ttcgaagccc gcctggccgg tggcgcagaa cccgcacgcc atggcacagc
     8341 cggcctgggt cgacacgcac accgtgctgc ggtcgtcgta gtgcatgagc acggtctcga
     8401 tccgtgaccc gccgtcgagc gcccagagga acttgaccgt gcggccgtcg tcgccggtga
     8461 cgcgggtgac ctcgtcgagc gcggtgggca gctgggcggc caggcgttcc cgaagtgcct
     8521 tcggcacgtt ggtcagctcc gacaccgtcc ggccctggcg gtacagcccg tcccacacct
     8581 gctcgacccg gtaggcgggc tcgccggtca gcaactcgga gagctcgtca cggccgaggt
     8641 cgtagcgggt tcggcgcgac accggcccgg ggcggctccc gccgggggtg ctcacgaggt
     8701 ggtctccggg tccgggttcg tcgccggtcc ggcggtgccg tcggtgccgt cgtgtcggcg
     8761 ggccggcgtg taggcggcgt tcgagtggtg caccacgaag cccagtcgct cgtagaggcg
     8821 cagggcggcg gtgttgaccg cttcgacgta gagcatcccg acgcgcaggc cccgctcggt
     8881 caggtggtcc aggcccgcga cggtgagtgc ccgtcccaag ccggtgccgg cggtcgacgg
     8941 gtcggtcgcg atcacgaaga tctcacccat cgacgggtcc tcggtgccgt ccggtgcggg
     9001 gccggccggg tgctcacggg tccagcagaa cccgtcgagg tcgccgtcgg ctccgtcgtg
     9061 gacgaggaac ccgtcggcgc ggacccacgg ctcggcgaga cgagccgtga ggcgggcacg
     9121 gtcccatccg ccctggtcgg ggtgccagcg gaaggcccgg ttgttgaccg acagccaggc
     9181 gtcgtcgtcc gcgccgggct cgaaggcccg gacgtcgatc gaccgggtgg atgcgagaac
     9241 cgatccggcg agcggcaggt ccacccgcat ctggtgcagg agacgcagca tcgagcgacc
     9301 ctctgagcgc atgaccgcgt cgacatcggg cgtcaccgcc gacacccatt cgggctcggg
     9361 tcggggatcg ctgggtccac gctcgaacgc ggacgcatca tcggccatcg cagcgacgct
     9421 acccgggcca cgcggcgacc ggatctgcga catggcgccg acgccgtgaa cgatcggacg
     9481 accggtcgtg ggagtcgtgg acagctcagg gtcggtgtgc tgaactgacc cgcaccaggt
     9541 tcccgccacc tggtatcggc gaccagtggg ctcccgccgc ccgggtcgcc cggccggcgc
     9601 tccttcgggg gcgccgtgcc cgttttcgcc ccggtcgacc gacgcggtcg tgccacgann
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cgttctgtag tgcgagatcc
     9781 acacgaacgt gtggatctcg cactacagaa cggcagggag tggtcggaac ggcaaggagg
     9841 ggtcgcgtcg gcggtcaggc ccggggttcg acgaagtgct cggccgcctc gagcgcccgc
     9901 cgcaatcgtc gttgcgccgc ctgcaggttc cgctccacct cgtagaggtc gccgagcagg
     9961 tcgtcgcggt cggtcacccg caccaccgac gcggcctcgt cgacgcgggc cagcacctgg
    10021 tccagggtcg actgcatcga tgcgaggtcg gcgtgcaggc tcacggccgg attcctacta
    10081 ctggccgccg cggcgccacc actaccgtgg accgccgtgc tcaaccgact cgacctccgc
    10141 ggcgtccccg atccctccgg ttcgctgccc cgcccacagg tggacggcga cgagccggtg
    10201 gctgcggtgc ggtcgatcct cgccgaggtg cgcagacgcg gcgacgccgc cctgatcgag
    10261 ctcaccgagc gcttcgacgg cgtcgtgctc gagcgggtgc gagtgccggc cgaggacatc
    10321 gacgcagcgc tcgccgcgac gcccgacgac gtgcgcgtcg cgctggagct ggcccgcgac
    10381 cgcatccgtg cacaccacga gacgcagctg cgcgccgagg tcgtccacga caccgacggc
    10441 gtgcacatcc gttcgttcca ccgccccgtc gaccgggtcg gctgctacgt gccgggtggc
    10501 cgcgccgcgt acccctcgac gctgctgatg acggcggtgc ccgcccaggt cgcaggggtc
    10561 ccccgggtgg cggtgtgcgt cccgcccgac cgcgacaccg gccgcgtcac tccggtcacg
    10621 ctggccgccg ccgcggtggc gggcgtggac gaggtgtacg ccatcggcgg cgcccaggcc
    10681 atcggagcgc tcgcctacgg caccgagcag gtgccggcgg tcgacgtgat ctgcggcccg
    10741 ggcaacaagt acgtggcgct ggccaagcag gaggtcgccg gcaccgtcgg tgtggccgcc
    10801 gcgttcgcgg ggccgagcga ggtcgtcgtg atcgcggacg ggaccgtccg tccggagctg
    10861 gcggccgtcg acgtcatcct ccaggccgag cacggccccg acggcctggc ctggctgatc
    10921 tcctgggact ccgacgccct cgacgccatc gacgtcgagc tcgcccggct gaccgaggcg
    10981 gcgccccgcc gggccgagat caccgccacg ctggccgacg gcggctacgc cgtgctggtc
    11041 gactcgccgg ctgccgcggt cgaggtcgcc aacctggtcg cccccgagca cctcgagctg
    11101 ctctgcgtcg gagccgagga cctggtcgca gcggtccgca acgccggggc gatcttcgtc
    11161 ggcccgtgga gcccggcgtc ggtgggcgac tacgtcgccg ggcccagcca cgtgctgccg
    11221 acctacggca ccgcccgctt ctcctcggcg ttgaccgtcg acgacttcct caagcaccac
    11281 cacgtgatca ccgtcgacgg cgacgcgttc gaccgcatcg gcccggccgt gcagtcgttg
    11341 gccgaggccg aggggctcga cgcccacgcc gagtcgatcc ggatccggcg cgacctgcgc
    11401 gaccggggga tcgacggcca ccggggagcg acgtcgtgag ccggccgccg gtgcgcgacc
    11461 ggatcgcgct gatggacggc taccactcac cgcaggtcga tgtccgggtc cggctcaaca
    11521 ccaacgaggc acccgagccg ccgccggcgg cgttcgtcga cgcgctcgcc gccggtctgt
    11581 cggggatcga gtggcaccgc tacccggacc gcgccgcggt tgctctgcga tcgcgcatcg
    11641 ccgatgtcga gggtgccgag acccccggac cgatcgacgc cgccaacgtg ttcgccgcca
    11701 acggctccaa cgaggtgctc cagtcgatct gcctggccta cgggggtgcc ggccggaccg
    11761 tcgccacctt cgagccgacc tacgccctgc actcccacat cgcccgggtg tgcgggtccg
    11821 acgtcgtctc cgcggcccgg gaggcggact tcaccctgga cgtcgacgtc gcccgacgcc
    11881 tgctactcga ccgacgcccg acgatcacgt tcctgtgctc gcccaacaac ccgaccggcc
    11941 gcctggagcc cgaggagacg gtgcgcgccg tgctggagac ggtcgaggag atcggcggcc
    12001 tgctggtcgt cgacgaggcg tacggccagt tctcgccgtg gtcggcgttg agcctgctgt
    12061 ccgaggaccg gccggtggtc gtgacccgga cgttctccaa gacctggtcc gcggccgcgc
    12121 tccgtctggg atacctgatc ggcccgtcct gggtcgtcga ggacctcgag aaggtcgtcc
    12181 tgccgtacca cctcgactcg atgacccagc tggccggggt gctcgccctc gaccacctcg
    12241 acgacatgca gcgacgggtg gcgcacctgg tcgaggagcg gggccggctc gaggccgcgc
    12301 tgagctcgct cgaggtcgac atgtggccga gcgccgcgaa cttcatcctg ttccggcccc
    12361 gcggccgtga cggcgccgag gtctggcagg agctggtcga ccgcggcgtg ctggtgcgca
    12421 actgcgcgtc ctggcccggc ctggagggct gcctgcgcgt cactctcggc accgtcgagg
    12481 aggacgacga gttcctggct gcgctcaccg aggtcctgac cggccggtga gcgcaccggg
    12541 ccgcaacgcc gggtcggtcc aggcgatcgc gttctggtgt cggtgcgccg acgcgcgatc
    12601 cttgaccccg tgagcgacac cccccgcacc gcccaggccg agcgcgtcac caaggagacg
    12661 acgatctcgg tcacgctcga cgtcgacggc gacggcacca ccgactgccg caccgggctg
    12721 ccgttcttcg accacatgct cgaccagctc ggtcgccacg gcgggatgga cctcaccctg
    12781 cacgccgacg gcgatctgga gatcgacgcg caccacaccg tcgaggacac cggcatcatc
    12841 gtcggcgagg cgctgcgaca ggccctgggc gacaagcgcg gcgtgcgtcg cttcgcgtcg
    12901 atccgggtgc ccctcgacga agcggtgatc gaagcggtcc tcgacctgtc gggtcggccg
    12961 tacctgcact acgacatcga cccgcccggc gtgaagatcc tgggcgaccc gccgttcgac
    13021 ccccagctga tggaggagtt ctggcgggcg ctggtcacct cggccggcat caccctgcac
    13081 ctcaccctgg tccgcggtcg caacacccac cacatcatcg aggcgtcggt gaaggccgcg
    13141 gcccgcgccc tgcgcgacgc cgtgcgggtg gagggcaccg agctgccctc caccaagggc
    13201 acgttgtgac cgtggaggcc ggcccgcgga tcgccgtgct cgactacggc atcggcaacc
    13261 tgcgttcggc cgagaaggcg ctcgagcacg tcggcgcgga tgccttcctg accgcggacc
    13321 ccgaccgggt cgagagggcc gacgcggtcg tcctgcccgg cgtcggagcg ttcggacgct
    13381 gccgggaggc gctgtcggag tcgggtctgg accaggtcgc gctcgacgcc gtggcctcgg
    13441 gccgaccgtt cctgggcatc tgcgtgggca tgcagctgct ctacgaggag tccgacgagt
    13501 cgcccggcgt gaccggtctc ggcgtcctgt cgggccgggt ccagcgactg ccggcggggg
    13561 tcaagcaccc gcagatgcag tggaaccagg tcgacctggt cagcgagtcg ccgctgttcg
    13621 acggactggg cgagcgcccg tggatgtact tcgtgcactc cttcgccccc gaggtcaccg
    13681 gcgacgtcat cggcacctgc gactacgggg gagcggtcac cgccgcggtg cagcacgaga
    13741 tggtgttcgc cacccagttc caccccgaga agtccgggac ggccggcctc gccctgctgc
    13801 ggaacttcgt ccgcctggcc gccaccgcga ccgtcagctg atccgcaccg cccacccgag
    13861 agcttcagag gaccacgaag gcaacgatgg acctgtaccc cgcgatcgac ctgctcgacg
    13921 gccgttgcgt ccggctgtac cagggcgact acgaccgcag caccgtctac ggcgacgacc
    13981 cggtcgccca ggcgctggcg ttccagtccg agggcgcgcc gtgggtccac gtcgtcgacc
    14041 tcgacgcggc gcgcaccggt gaaccccgca accgcgacgt cgtcgccgcc atcgccgggg
    14101 cgctcgacgt gccggtgcag accggcgggg gcgtgcgcac cgtcgaggcg gccgaggcgc
    14161 tgttctccgc cggcgtcgcc cgcgtcgtgc tgggcacggc cgctctggag gaccccgacc
    14221 tggtccggcg cctggccgcc gatcacccgg tcgcggtcgg cctcgacgcc cgcggatccg
    14281 aggtggcggt ccggggctgg ctcgaggggt cgggcgcgga cgtgttggac ctggcggtcg
    14341 cgtaccagga cgccggggtc gcggcgctgg tggtcaccga gatcagccgc gacggcaccc
    14401 tcgaggggcc cgacctcgcc ggcctggccg cggtggtcgc cgcgaccaag gtgccggtga
    14461 tcgcctcggg cggcatcggc accctggccg acatcgaggc gctcgtcgcg ctggcggccg
    14521 gagggcgctc gctggcggga gcgatcaccg ggcgggccgt ctacgaaggc gcgttcaccg
    14581 tggcccaggc cgtcgccgtc acccggggga gtgcgacatg accgcggtgg cccgtgtcat
    14641 cccctgcctg gacgtcgacg ccggccgggt ggtcaagggc gtcaacttcg tggcgctgcg
    14701 cgacgcgggt gacccggtcg agctcgcggc gcgctacgac gccgagggag ccgacgagct
    14761 cgtgttcctc gacatcaccg catcgtctga tgcccgcgag accacgatcg acatggtccg
    14821 ccacaccgcc gaagaggtgt tcatcccctt caccgtcggc ggcgggatcc gcagcgtgga
    14881 ggacgcccgt cggatcctgc gcgccggcgc cgacaaggtc ggcgtcaaca ccgcagcggt
    14941 ggaccgcccc gagctggtcg ccgagatcgc cgatgagttc ggcgcccagt gcgtcgtcgt
    15001 cgccatcgac tcccgcgccg gggacggcaa cggtccccgc ttcgaggtcc acacccacgg
    15061 cggtcggacc ccgaccggtc tcgacaccgt cgagtgggcc gcccgggtcg ccgagctcgg
    15121 agcgggcgag atcctgctga cctccatgga ccgcgacggc accaaggacg gcttcgacgt
    15181 ccccgtcacc gccgcggtct ccgacgcggt caacgttccg gtcatcgcgt cgggcggtgt
    15241 cggacgcgtc gagcacatgg tcgagggcat cactgtcgcc ggagccgacg ccgtgctcgc
    15301 cgcgtcgatc ttccacttcg gtcagcacac cgtcgccgag gtcaaggccg ctctcgccgc
    15361 ggccggagtt cccgtccgac ccgtctgagc gaccggtccg gaccggccgg ggtacggtgc
    15421 cgcgatggag ctgcgcgtct tcaccgaacc ccagcaggga gccacctacg accagctgct
    15481 cgccgtggca cgggcggccg aggagctcgg cttcggcggc ttcttccgct ccgaccacta
    15541 cctggccatg ggcgacaccg ccggactgcc cggtccgacc gacgcctgga ccaccctcgc
    15601 cggcctggcc cgcgacacgg cgaccatccg cctcggcacg ctcgtcaccg ccggcacctt
    15661 ccggctcccg gggccgctcg ccatcaccgt cgccaccgtc gacgccatgt cgggtggacg
    15721 agtcgagctc ggtctgggcg ccgggtggtt cgacgacgag cacaccgcct acgggatccc
    15781 gttcccgccg ctcggcgagc gcttcgaccg gctgaccgag cagctcgaga tcgtcgacgg
    15841 gctgtggtcc accccggcgg gcaagacctt cgaccacgac ggccgccact accagatcgc
    15901 gggcagcccc gcgttgccca agccggtgca gcggccccgg ccgccgatca tcgtgggcgg
    15961 cttcggggcg cgacgcaccc ccgccctggt ggcgcgattc gcagacgagt acaacgtgcc
    16021 cttcgccgat gccgacaccg tcgcggcgct caaccgaggc gtcgacgagg tgtgcaggtc
    16081 ccacgaccgg gtcccggcca cggtgctgcg ctcggcggcc caggtgctct gcgtcggtcg
    16141 ggacgaaccc gagatcgccc gacgcgccgg cgccatcggc cgtgaggtcg acgagctgcg
    16201 caccaacggc ctggccggca cccccgacga ggtgatcgcc aagatcgagc gcttcgcatc
    16261 gatcggcatg acgcggttgt acctgcaggt gctcgacctc gacgacctcg agcacctggc
    16321 cctggtcgcc gagaaggtca tgcccgctgt cgcctcgtcc tgacgctcgc cgtcctgggg
    16381 ctcgccgtcc tgaggtgtcc tgaggggtcc tgtcgcgggc ggcaggactg ccgagtaccg
    16441 acctcgcgac gccaccggta gcgtggtcga cccatgaccg acgccgaccc caccgacggc
    16501 taccccgccg acgccgaccc cagcgacggc accggccccg acggcgcggg cgccgatggg
    16561 acggcggcag cctcgccgtc cccgcctcgg ccgaccgggt cgcacgagaa gctgccggtg
    16621 cggatgctca acgaccgcat cctggtcaag gtcgatgccg agggggaccg ccgctccacc
    16681 gggggaatcc tcatcccggc caccgcccag atgggcaagc gcctggcgtg ggccgaggtc
    16741 cgggcgaccg gccccaacgt gcgggccgtc gaggacggcg accacgtcct gttcaacccc
    16801 gaagaggtct acgaggtcga ggtccggggc gacacgttca tcatcctgcg cgagcgggac
    16861 atccacgcgg tggcctccga gcgcctcgaa gccggccaca ccgggctcta cctgtaggac
    16921 cggcggaatc tgtaggaccg cggaaccacg cactcctctg gacgcaggac ggcgccgccg
    16981 attccggttc ggctccctcc ggcggccgct cctacgattg gcccaccatg tgccccgctc
    17041 ctgcgccctc gtccgacacc ccgcccgccg tgacccccgt cgcggtgcgt gacgccgatc
    17101 tggagctcat ccgctacgac tccgacggcc tggtcccggc catcgtgcag gacgtcgaga
    17161 cccgggcggt gctgatgatg gcgtggatga acgccgagac gctgcgcatg agcctggagc
    17221 agggccgaac cgtgttctgg tcccggtcgc gccaggaggt gtggcgcaag ggcgacacct
    17281 ccggtgaccg acagttcatc cgttcggccc actacgactg cgacggcgac gcgctgctgt
    17341 tcctggtcga gcaggagggc aagggggcgt gtcacaccgg cgcctacacc tgctggttcc
    17401 gcaccttcgg atccgacgag cagaccaccg accgaccggg accggtctga ggcccgcggg
    17461 ccgatcgacg ccgccggatg ctgacccgag accagttcca ccgcctggcc cgtgaccacc
    17521 gcgtcgtccc ggtcacccgg acgctgctgg ccgacctgac caccccggtc gccgcgttcg
    17581 cccggtggtg cggccccgac tccccggcgg acgggagtcc ggcccgggcg ggcttcctgc
    17641 tcgagtcggt cgaccacggc ggccgctggg gtcgctggtc cttcgtcggc cgcgaccccc
    17701 ggttgcgcct cgtgtcccgc agcggccggg tcgaggtcga tggatcgctc cccgacggcc
    17761 tggtcggtga tctccccacc ggggacggga tcctggccgt gctcgaggtc ctgctcgagc
    17821 acctgcgggc tccgaccccc gacgctctcg ccgccgtcgt cgggacctcg gacgcagccg
    17881 acggaccccg cggttgggag ctgccgccgc tgcactccgg ggtcatgggg tatctgggct
    17941 acgacgtcgt gcgcgaggtc gagcacctgc cggcggtgcc cgccgacgac cggggctggc
    18001 ccgacgccgt cctgtcggtc atcggcgaga tcgccgcgta cgaccactgg tcccagcagg
    18061 tgaccctggt cgccaacgcg ttcgtcgccg acggcgcgga ccggtccgcg gtcgacctcg
    18121 cctacgacga cgcggtggcc cgcctggagc gcatggccgc cgacggtgcc accccgatcg
    18181 acgagccgct ggtcgaaccg cccacccggg ccgacgagtc ggtggcggtc acgtcgtcga
    18241 tgtcgggcgg gacctacgag gcggcggtcg acgcggccaa ggagcacatc ctcgccggcg
    18301 acatcttcca ggtcgttctc gctcagcgct tcgacttcac cacgacggct gaaccgctcg
    18361 acatctaccg gacgctgcgc caggtcaacc cgagccccta catgttcctc atgcgcgagg
    18421 acgacctgac cctggtcggc tgctcgcccg agccgatggt gcagctgctc gacggccgtg
    18481 tgatctcccg ccccatcgcc ggcacccggt tccgggggcg caccgacgag cacgaccgcc
    18541 gcctggccgc cgagctggtc gagcacccca aggagcgggc cgagcacatc atgctggtcg
    18601 acctggcccg caacgacgtc gggcgggtcg tcgagttcgg cacctgccag gtcgacgagc
    18661 tgatgaccct cgagcgctac agccacgtca tgcacctgac ctcgcaggtg tcgggtgctc
    18721 tggccgaggg gtgcggcccg atcgacgtgc tgcgggccac gctgccggcg ggcacggtgt
    18781 cgggcgcgcc caaggtgcgg gccatggaga tcatcgacga gctcgaaccg gtcaagcgcg
    18841 gcccgtacgc cggcatcgtc ggctacgtcg acttctccgg caacatcgac accgccatcg
    18901 ccatccgcac gatgctggtc gacccgcccg gagctgacgg gacccgtcgg gcgtgcgtgc
    18961 aggccggcgc cggcgtcgtc gccgactcgg tggccgccga cgaggagctc gagacccgca
    19021 acaaggccag ggcgctgctg tcggcggtgc cggccgccga gcgcatgacc gcgtaccgtc
    19081 gcgccgccgg aggtcgttga tggccactcc ggccgcgccc gacgagggcg tcgtggccgg
    19141 agtgggcgcg gcccgctccg acgtcgtcgc cgtcgccctc gagcgcgacg gcctggtcgt
    19201 caccgggtcc gacgcggtgt cgttcgtcca gggccaggtg tcggcggacg tgtcctcgct
    19261 ggcggtgggc gaaagcacgt ggtcgctgct gctcgagccg cagggcaagg tggcggcgtg
    19321 gctgcgcatc acccggaccg ctgacgccga gctgttcgtc gacgtggacg ccgggtgggg
    19381 ggagtcgacc ctggcccggc tgcgccggtt cctgctgcgc gtcgacgtgg tcgtcgagcc
    19441 ggtgcggcgc cgcggcctgg cactggtcgg gcctcgggcg gccgacgtcg ccgacggagc
    19501 gtccggggtc gagctgtcgc tggcggtcga ccgccgtggt ctggccggag tcgatctgtt
    19561 cggcccggtc gacggaccgg caccggagcc gcccgacggt gtgcagcggg tcgggcccga
    19621 ggtcctcgag gtgctgcgga tcgagcaggg gtggccggtc atgggccgcg agctcgacgc
    19681 ctcgaccatc cccgccgagg cgggggcgtg gctcatcgac acctcggtca gcttcaccaa
    19741 gggctgctac accggccagg agctggtcgc ccgcgtgcac caccggggcg acaacgtgcc
    19801 gcgccacctg cggggcctgg tcatcgacgg ggcggtgctc ccgagccccg gtgcaccggt
    19861 catcgccgag ggcgtcgagc ggggagcggt caccagcgcg gtcgtgtcgc ccacgctcgg
    19921 aggcgtgccg gtcgcgctgg cgatggtgca ccggcgagtc gagctcggcg cggacgtcga
    19981 gttcgacggc gcgacggccc gcgtggtcga cctcccgttc gtcgccccgc cccctccgaa
    20041 cttgtagcca gcggtcgcca gggcgacgcg tgggctacaa gttcgccggg ccgtgtcgaa
    20101 cttgtagctg gcggtcgcca gggcgacgcg tgggctacaa gttcgatggg ggagcgacgg
    20161 gggtcagcgg gacccggaac cagaacgccg tgccgccgtc ggcgggcgcg tggacgccga
    20221 ccgtgccgcc catggcggtg accagctccc ggacgatggc cagacccagg cccgaaccgg
    20281 actcggatcg ggtcggtcga cggcgcccct ggtagagacg ttcgaacacg aagggcaggt
    20341 cggccgcgtc gatcggggtg ccgtcgttga tcacgtggac cacgaccgag cggtggcccg
    20401 tcgttgccga cggatcgccg gcggattcgg cggattcggc gggttcgccg gacgcggtga
    20461 ccgtgaccgt tccggccgcg aaccgggtgg cgttgccgac caggttgccg acgacctgcg
    20521 ccagacggtc cgggtccacc gtcccggtca acccggcgtc ggcctgcacc accaggctca
    20581 ggccggccgc cgccaactcg gcccgatggg actcggctgc gtcggcgacc acggatccga
    20641 cgtcgaccgc gcgggggtgc agggtgaact gtcgggcgtc gagtcgggcc aggtccaaca
    20701 ggtcgcgcac cagacgctcc aggcggtccg actcgcggct gatgacccgg gcgccgtccc
    20761 ccggtgcggt cgcgccgtcg gcgatcgcct cggcgtagcc gcggatgttg gtcagcgggg
    20821 tgcgcaggtc gtggctcacc gacatcagga actgctgctc caggccccgg gaccgggcca
    20881 gggcgtcggc catggcgttg atcgaccggg ccagcacggc ggtctcgtcg ccggctcccg
    20941 ggaacgggtc gggcaggcgt gcgtccagat ctcccgccgc gatccgttcg gtcaccgcgg
    21001 tggcgtcgcg caccggcctc gccagccggg aacccagcca cagcccgacc ccgacggcca
    21061 acgccaccac caacgccgag gagatcacga accacccggc gggcccgctg agcagacccc
    21121 gggtggtgcc ggtcatgccc acgacgatca ccgcgggccc gacggacctc ccggtggcgg
    21181 cgtacaggcg gcccccggcc gactcgcccg acaccagtcc accggcacgg accgcttcct
    21241 cctcggtcgg ggtgagcgtg acccccgcgg gcagctcgcc ccggctgcgt ccctcgggtc
    21301 cgacgatcac cgcgccgatg tcgtgcaggt cgaacgcgtc ggacatcctg cgcagcgacg
    21361 tcaccgacag tcgggggttg ccctcggcgt cgggttggtt ggcggttccc aacgccgccg
    21421 ccagatcggc caccaccccg gcctggcgtc ggaggtccgc ctcggcgctt cggcgtgccg
    21481 cggcccggct caacaccagc gtgccgagcc cggcgagcgc cagggcgacg accaccgaca
    21541 cgatcacagc ggcggacact cgtcgacgca tcccggctcc tcgtcccgtg gtccggccgt
    21601 cagtccagcc ggtaaccgac gccccacacc gtgacgaggg gcaggtcgtc tccgagcttg
    21661 cggcgcagct gaccgacgtg gacgtcgacg gtccgttcgt cgccgatcca gccgtcgccc
    21721 cacgcgccgt cgaggatctg ccggcggctg agggcgaggc cgcggttggc gcccaggaac
    21781 gccagcagct cgaactcctg ggtggtcaga cccaccggtt cgccgtcgcg tcgggcctcg
    21841 tggcggccga ggtcgacctc gacgctgccc acctcgatca ccgccggcgc aacagtcgtc
    21901 gccgcggcgg tcgctgccgc ccgtcgaagc tgggcccgga cccgggccac cagctcccgg
    21961 ggcgagaacg gcttggtcac gtagtcgtcc gcgcccatct ccaggccgag aacccggtcg
    22021 atctcgtcgt cgcgtgcggt gaggaacacc accgacaccc ccgcgtccga ggcccggatc
    22081 cggcggcaca cctcgagacc gtcgattccg ggcagaccca cgtcgaggat caccacgtcg
    22141 gggcggtgcg cggcgatcag ctcgaggccc cgctccccgt ccgcggccag gtagacgcgg
    22201 tggtcctctc gtcggaggta catgtcgagc agatcggcga tcgccggatc gtcctcgacg
    22261 gccacgatcg ttgccaccga cccgttctat cggaggatga ccacggagcg gtcaggagcc
    22321 tgtgatcgaa gtgtgacgcg ccgtctaccg tcgcggaccc atggccacct acctggaccg
    22381 catcctcgat cgtcaccggg ccgacgcggc cgcggacgag cggcgcaccg aggacctggt
    22441 gcaggccgcc ggcgagctcc caccgacccg aggcttccgc tcggccctgc tggcggggac
    22501 ggagctggcg gtgatctccg aggtcaagcg ccgctcgccg tccaagggcg acctgttcgc
    22561 ggacctggac ccggccgcgc tggccgcgga ctaccaggcc gccggggcga gctgcctgtc
    22621 ggtgctgacc gacggcgagt ccttcggcgg gtccgccgac gacctggccg cggcccgggc
    22681 cgcggtgagc gtcccggtgc tgcgcaagga cttcaccgtg tcggccaacg acgtgtgcga
    22741 cgcccggctg atgggggccg actgcgtgct gctcatcgtg gccgcgctca gcgacggcga
    22801 gctcggcgag ctgtcggcgc tggcggtgca gatcggcctc gacgtgctgg tcgaggtgca
    22861 cgacgaggcc gagctggaac gggctctcgc caccaccgcc ggggacctcg tcggggtcaa
    22921 ccagcgggac ctggtcacct tcgaggtcga caccgcccga gcggtccggg tcgcggcggc
    22981 catgcccgcc ggcacggtcc gggtggccga gtcgggggtg cgtggccgcg acgacgcgct
    23041 cgcgctcgcc gccgcgggct accacgccgt tctggtcggc gagcacctgg tcaccgctgc
    23101 ggaccccggc gccgcgctcg ccgcgctgcg ggttccccgg gggccgcgcg gcggttgaaa
    23161 ccctccacga accgtcgcgt cggtgccgat agcgttcgag ccgtgttcgt gaagatctgc
    23221 ggcatcacca acgaggagga cgccctgatg gcgaccgccc tcggagccga cgccgtgggc
    23281 ttcgtgttcg cccccagcgc ccgccaggtg agcgtgggga ccgtgcgcga catcgtcaag
    23341 cggctcccac ccgaggtggt gaccgtcggc gtgttccgcg accaggcccc ccggctggtg
    23401 atcgacacgg tcctcgaggc cgggttgcgg gccgtgcagc tccacggcca cgagtccccg
    23461 caggacgcgg cggagatccg gccctacgcc cacgccctga tcgtggcgct cgcggtcggc
    23521 gacccggcgc tcgcccacgt cgacgactac ggcgcggacg ccctgctgct cgactcgccg
    23581 gtcccaggtt ccggcaacgt gttcgactgg gccctggccg agggtgttcc ggtcgggcgg
    23641 cggatgatcc tcgccggcgg gctcacaccc gacaacgtcg cagccgcggt cgccgaggtg
    23701 cagccctggg gggtggacac ctcgaccggc gtccaggccg acggcgaccc gacgcgcaag
    23761 gacccgctca aggtccagcg cttcgtccgc agcgcccgcg ccgcggcgcc ccggccgtac
    23821 caccccgagg ggaacggtcc cttcgactgg gccgacgccg ggctctgagc ccgacgatgc
    23881 cggaacccga ttcgggccgc cggaggtgcg gcgctaccgt gccgcccgtg accgaacccg
    23941 tgagcgcatc cggcgagccg gtgatgggcg agcccgaccc gaccgggcgc ttcggcgacc
    24001 acggcggccg cttcgtgccc gagtcgctga tgccggcgct gatcgagctc gacgccgcct
    24061 tccgtgacgc gtgggccgac cctgcgttcc gctccgagct cgacgagctg cttcgctcct
    24121 acgccggccg tccgagtccg ctcaccgact gccaccgcct gtccgaggag gtcggctgtc
    24181 gggtcctgct caagcgcgag gacctcaacc acaccggttc gcacaagatc aacaacgtgc
    24241 tcggtcaggc gctgctcgcc cggcgcatgg gcaagacccg cctggtggcc gagaccgggg
    24301 cgggccagca cggcgtcgcg acggccaccg ccgcggcgct gctggggatg gactgcctgg
    24361 tgtacatggg tgaggtcgac atggcccgcc agcagctcaa cgtgttccgc atgcgtctgc
    24421 tgggagccga ggtgaccccg gcgacctccg gcagccggac cctcaaggac gccgtcaacg
    24481 aggccatgcg tgactgggtg gcgaccgtcg agtcctcgca ctactgcctg ggatcggtga
    24541 tgggcccgca cccgtacccg tggatggtgc gtgagttcca ccgggtgatc ggcgtcgagg
    24601 ccgccgcgca gtgcgacgac ctgctgggcg ggccgcccga cgtggtggtg gcctgcgtgg
    24661 gcgggggttc caacgcggcc ggcatcttct cgggcttcgc cgaggtgccc ggcgtcgagc
    24721 tggtcggcgt ggagcccgcc gggggagcgg cgatcggcgg gggtgtcccc gggatcgtcc
    24781 acggcatgca cagctacctc aagcaggacg agttcggtca ggtgctcgaa gccgagtcga
    24841 tctccgccgg gttggactac cccggcgtcg gcccggagca ctcgcacctg gccgacgtgg
    24901 gccgggcccg ctacgagccg gtcaccgacg acgaggtcgt cgacgccttc gtgctgctca
    24961 gccgcaccga ggggatcatc cccgctctcg agtcggccca cgccctggcc tgggtgtcgc
    25021 gggcccgtgc cgagctggcc gggcggaccg tgctgatcaa cctgtcgggg cggggcgaca
    25081 aggacgtggc gcagatgatg gacgtgctgg cggaccggct gtgagcgccg tcgccctcga
    25141 cctggaggcg acgctgcggg gacgccgcga cgccggggcc aagacgctgg tcccgtacct
    25201 gaccggcggg ctgttcgcag gggccgacgg cgactggacc gacgtgctgc gctcggtggc
    25261 gtccgccggt gccgacgcgg tcgaggtcgg gatcccgttc tcggatccgg tcatggacgg
    25321 gccggtgatc caacaggcct cagagctggc cttggcttcg gtggcgaccc ctccgggcat
    25381 cctcgacgag ctcgcagggg tcgacgcggg cgtgccgttg gcggtgatga cctacgtcaa
    25441 cctggtctac cggttcggct acgagcgctt cgcggcgacc gcccgccgct gcggggtggc
    25501 cgcagccatc gtgccggacc tgccgctcga ggagtccgag ccgtggtgcg cggccgccga
    25561 cggggccggc atccagaccg tgatgctcgc cgcgccgacc gctccggacg agcgcctgga
    25621 acgaatcgtg gcccgggccc gcggcttcgt ctacgccgtc gggctgatgg gggtcacggg
    25681 ggagcgcgct gcgctggccg actcggccac ggtgatcgcg tcgcggctca aggcgatcac
    25741 cgacgtgccg gtcctggtcg gggtcggcat ctcgtcgccc gagcaggcgg tcgaggtctg
    25801 cgaggtggcc gacggcgtcg tcgtgggcac cgccctggtg cgccgggtgc tcgagacccg
    25861 ctcaccggag gccgccggcg agctcatcgg ctcgttccgc gacgccctcg accgcggctg
    25921 agagccccga ccaggcgcgt gagcatctgc gcgcgaagcg cgcgaggccc ggaattccaa
    25981 gggaaaagct cgcttcggac ccctccgacg tgatagttac atcgtggtaa ttcaatcgtg
    26041 atcgtcgacc agtcctccgg ggggtcgacc aggaggaacc atgaacaaga ccgaactggt
    26101 ggagaccatc tcccagcgga ccgacatcgc caagaaggac gtccaggccg ttctcgacac
    26161 cttcgaggac atcgccggtg agatcgtcgc caagggcaag gacacgctga cgatcccggg
    26221 cttcctgaag ttcgagcaga cgcaccgggc cgcccgcacc ggtcgcaacc cgcagaccgg
    26281 cgagaccatc caggtcccgg cctccaacgc cgccaaggtg tccgccggcg ccaagctcaa
    26341 gaaccgcgcc aagaccggca agtgatctgc ggtcggcctc cgggccggcc gctcgctggg
    26401 gacccgctgg ggtcagtgat ggagccgccc tgtcggggcg gctctgtcgc gtcccggttc
    26461 gggtcggggg taggttcccg gccatgacca agctcgagat cggtgacaaa gcccccgcga
    26521 tcggactgaa ggaccaggac ggcgagacgg tgcgcctgtc gtcgttcaag gggcgcaagg
    26581 tgctggtgtt cttctacccc aaggccaaca cgccgggctg caccaaccag gcctgcggtc
    26641 tgcgcgacgt ggccgaccag gtgggcgaca ccgccatcgt cggcatcagc ccggacatgc
    26701 ccgacaagca ggccgcgttc gaccagaagt actcgctcgg ctacccgctg ctgtcggacc
    26761 ccgagcacaa ggtcgccgag gcgtacgggg tgtggggcga gaagaagctc tacgggcgtg
    26821 ccttcatggg gatcatccgc tcggcgttcc tgatcgacga gaagggcaag gtcgagcagg
    26881 cctggtacaa gatcagcccc aaggacacgc ccctcaagct cctcgccgcc ctgaaggact
    26941 gagcggcatc ggcggcctgg ccgctggcgt tgttgtagct ggcaggtcgc ctggcgaccc
    27001 gtggcctaca agttcggcgg ccgctggcgt tgttgtagct ggcaggtcgc cggacgaccc
    27061 gtggcctaca agttcgg
//
LOCUS       DAOQOD010000020        26834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_593_length_26834_cov_12.001307, whole genome shotgun sequence.
ACCESSION   DAOQOD010000020 DAOQOD010000000
VERSION     DAOQOD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 26834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26834
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_26834_cov_12.001307"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLZ72_03685"
     CDS             complement(<1..537)
                     /locus_tag="PLZ72_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNA35434.1"
                     /translation="MADIVMPQLGESVTEGTITRWFKAVGESVTEDEPLFEVSTDKVD
                     TEVPAPASGVLSEIRVQEGETVDVGTVLAVLGDGAGAAPAAAPAAPAEPAPAPAPAAP
                     APAPAPAPVAPAPAPVAPAPAPAAPAPAPAAAGGALLSPLVRRLIEENGLDPATIAGT
                     GVGGRITREDVLDVIDARA"
     gene            complement(611..2011)
                     /gene="lpdA"
                     /locus_tag="PLZ72_03690"
     CDS             complement(611..2011)
                     /gene="lpdA"
                     /locus_tag="PLZ72_03690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNA35435.1"
                     /translation="METTHFDLVIIGGGPAGYGAALYGASAGLNIALVEKDKVGGTCL
                     HRGCIPAKELLETAAVVRTIAHAEDFGIKVEPIGLEWSTTLARKQAVVDKLAGGVTGL
                     LKNRKVTILDGTATLGAGRTVTVVGGDESVTLTGDAVILAPGSVPRVIPGFEPGGPVV
                     TSDEFFHIPGVPERAVVIGGGAIGCEFASTLADLGASVTILEALPKILPGCDEDVTRV
                     VLQSFKKKGIDVRTGVAVSGHAPSSSGATTVSFGDGESIETDLVVVSVGRSPFTDGVI
                     AEGTGVRVTDRGHIEVDGNYRTGEPSVWAIGDVIATPQLAHVGFAEAICVVKDILGEE
                     AVPVDNSKVPWAIYCQPEVAFAGMSEAAAKEAGIEVVTSKHRFGGNSRAMIIGQTDGL
                     VKIIAEKRPDGSAGKLLGVHMVGPWVTEQLSGGYLAVNWEATADEVAAFIQPHPSLTE
                     LFGESVLALTGRSLHG"
     gene            2246..3019
                     /locus_tag="PLZ72_03695"
     CDS             2246..3019
                     /locus_tag="PLZ72_03695"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HNA35436.1"
                     /translation="MQDALGEVQSVLVLGGGSDIGRALCVRLARSRCRTVVLAGRPED
                     DMEPVADAARAAGATTVELVEWDSTDVDSHRKVIDDVFDRFGSIDLVYVPAGILGSQE
                     AFDADPTFAAKAVEINFGGLVSACLVVADRLEKQGYGTLVLMSSVAGLRARKDNFVYG
                     STKAGLDAFAQGLGDSLHGRGVRVVVVRPGFVRTKMTEGMDAAPFSTTPDKVAELIVA
                     GLAKGKEVVWAPPLLQYPFFVFRLLPRFLWRIVAERAGG"
     gene            3090..3611
                     /locus_tag="PLZ72_03700"
     CDS             3090..3611
                     /locus_tag="PLZ72_03700"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA35437.1"
                     /translation="MTTAPSAPPTAGDPSAATSSTPTSVSASRLIGAPAQQLFDLVAD
                     PTMHPVIDGSGSVRRVKGSARKLALGDRFTANMRIGVPYVIANTVVEYAEGRRIAWAH
                     FGGWRWRWEFEELEPVDGEPITRVTETYDWSTSRAGSSYPTLFGRDRKNQEAMTRSLN
                     RLAAYVAHSGQPL"
     gene            3645..4310
                     /locus_tag="PLZ72_03705"
     CDS             3645..4310
                     /locus_tag="PLZ72_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNA35438.1"
                     /translation="MTGSLAAFDFDGTITRRDTLVSFLARVAGPARFAGTCARLGLLG
                     ARGTIPVRDRDRVKEHMIELLLGGTAEHDLRTQGERYARDLLTGDRLRPEVVARIHEH
                     HRAGHRSVLVSASLVYYLEPIARELSIDAVIGVEPEVTDGVLTGRLARPNVRAEQKAI
                     RLREWLGAPALGPIEDTTIHAYGNSSGDHELLALADRAWWLGRESKCPDFARPFRPGT
                     ALS"
     gene            complement(4388..5176)
                     /locus_tag="PLZ72_03710"
     CDS             complement(4388..5176)
                     /locus_tag="PLZ72_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA35439.1"
                     /translation="MLELNGASALVTGGASGLGEATVRALAEAGSKVVIMDLGHQKEK
                     ADAILAEIGGGAFVECDVTNTDQVIASIDAAKEMGPLRAVINSAGIGWATRTIGRDGT
                     YESAHDLEIFRKVVEINLIGTFNVCRLAATAMSQNEPDEDGARGAIVNTASVAALEGQ
                     IGQVSYTASKAGVVGMTLTIARDLAAVGIRCNTIIPGLIDTPIYGQGEAAEQFKEKLG
                     AGTLFPKRLGKASEYASLALELLRNSYINGESVRIDSGMRMQPK"
     gene            complement(5241..5699)
                     /locus_tag="PLZ72_03715"
     CDS             complement(5241..5699)
                     /locus_tag="PLZ72_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNA35440.1"
                     /translation="MCDYCGCRESGPTAELADEHVRLAELSARLSAALAARQDVTALF
                     EEFTALLDRHAAKEEVGLFPKVLGLKVLDDDVAQLIAEHETLHRQLGHGPTGTEVGAA
                     LKLLAQHIDDEEFGLFPHIFHALDPEDWEEIELAHRAVDEVFDGPGPAAP"
     gene            5787..7325
                     /locus_tag="PLZ72_03720"
     CDS             5787..7325
                     /locus_tag="PLZ72_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNA35441.1"
                     /translation="MTTSADVSAGQSMRFEPTWESVSTHTVPSWYDDAKLGVFLHWGL
                     YSVPGWAPQVDDIQQMLRDEGPEAMLRANPYAEWYRSSMQIEGSPTQRHHRELYGDAP
                     YDDFITAFDDGSRSADLDELAGVCERAGARYVVLTTKHHEGFTLWPSAQRHPVKGAYR
                     AERDIVGELTGAVRARGMRMGLYYSGGYDWRWNDAVIARPADTTLAVPADPAYNAYAT
                     AHVRELIDRYAPSVLWNDVCWPAGGDLAALFAHYYNTVDDGVVNDRWLQPSGPRNRLT
                     DAVTSGLGELVQAAWQHLPERWKSLTFPGANHYDFNTPEYARFDEIRDKKWEATRGVG
                     HSFGANRNERPRDIVTTTELVRMLCDVVSKNGNLLIGIGPGPDGTVPDQQVAPLRGLG
                     DWLAVNGPAIYGTRPWDVAATTTSEGTEVRFTCRADGADGAGPAVHALLIDLPGRRTF
                     TLRDVDATDVDHVALLGLDEPIGWRVTDDARLEVTLPERLPVTAAYSLVLTGRPRSTA
                     AATD"
     gene            complement(7332..7763)
                     /locus_tag="PLZ72_03725"
     CDS             complement(7332..7763)
                     /locus_tag="PLZ72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35442.1"
                     /translation="MPTSRSRDLVLVDTSVAVALSVGDHERHSSSVEVLRGRRLGLAG
                     HAAFESFSVLTRLPAPNRRPASAIARLLAANFPDTRFMSPDATAGLLAELEGGEISGG
                     AVYDALVAAVARDHGAILATDDQRALDTYRVIGARIEVVNP"
     gene            complement(7747..7980)
                     /locus_tag="PLZ72_03730"
     CDS             complement(7747..7980)
                     /locus_tag="PLZ72_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNA35443.1"
                     /translation="MRATIDKAGRLVIPKPLRDRLGLRPGEVDVVVDGATLRIEVPTG
                     DTLESEGDFLVIPPSGEVLTAVQVQELRDADQS"
     gene            8073..9170
                     /locus_tag="PLZ72_03735"
     CDS             8073..9170
                     /locus_tag="PLZ72_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNA35444.1"
                     /translation="MVTTPDRAVMRWLFAFSGVVAALIVFGGFVRLTRSGLSIVEWKP
                     VTGVMPPIGEQAWHDAFAAYRRSPEFLIVNSSMTLDEFQRIFLIEWLHRLIARLAGFA
                     FLIPFAVFVARRRIPRRDLGPYLAMGSLFVLQAVAGWVMVASGLESRPSVSHINLSAH
                     LLLAFTLFALALWTGLDHRSAPVVPRRGAVWSRSSRLVAGFVAVLAVQILYGGFTAGL
                     KAGWVSNTWPLMLGAVVPETAFGSVADLVESPITVMWIHRWLAFAVAAMAGAAALGAR
                     ERRADTDVRRGTDAMVGLVCVQILLGIATVMTSVNMALALLHQATAIALFASGVFTLH
                     RLRSLDGFASVAPPLHDAPGDMSSTAGAARR"
     gene            complement(9064..10098)
                     /locus_tag="PLZ72_03740"
     CDS             complement(9064..10098)
                     /locus_tag="PLZ72_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HNA35445.1"
                     /translation="MDLRAEVMAVGSAWARALCRLVHLVRDLDSSGDWVLDGSRTCAH
                     WVASVLDVEVCTAREWLRIGRALAGLPEIDAAFSSGRISYSKVRQLTRIASPELEEEL
                     LEIAERVPAGRLGVELARWLAGREEPADTEDRHRSAREVSWRTDIDGMVLGRFRLAPA
                     DAAAVIAAIDSSVMAYRGPAHASADASAGAQRWPSVSQQRADALVGLVTGGGLGVTTE
                     VVLHVRGDGCSLDDGTPVAGSVLERLVPGSFLRALIHDAHGRPIDASGRHRHPSTRQK
                     RVVRERDQGRVDCGARNLLEFDHDPPYAVSGRTVIDELHLRCWTCHRARHAAGARPRR
                     TRRGSGADGG"
     gene            10554..10847
                     /locus_tag="PLZ72_03745"
     CDS             10554..10847
                     /locus_tag="PLZ72_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35446.1"
                     /translation="MSYGIVLEFEGVGEDAYWSANKRLGIDTDARTGYPDGLLVHAAG
                     PTASGWIVVEVWDERSSQERFMAERLGAALQAEGLPAPSRVTDSELVNAQALG"
     gene            complement(10868..11245)
                     /locus_tag="PLZ72_03750"
     CDS             complement(10868..11245)
                     /locus_tag="PLZ72_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35447.1"
                     /translation="MIDASAVVLGLMSDGEARAMLAAQAATCPHLVDSEVVHALRGRV
                     LRGDLDVGAAERMIDVWQRLGIERVPVGGLLGRVWELRENLSAYDATYVALAEALESP
                     LVTADGHLARAPGPQCTITVVRR"
     gene            complement(11248..>11463)
                     /locus_tag="PLZ72_03755"
     CDS             complement(11248..>11463)
                     /locus_tag="PLZ72_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35448.1"
                     /translation="TLYLRNVPDEVGERLERLAEREGLSLSAFATRELASISRRADNP
                     ALLAGLPDLRVDADAVVADVEAGRATR"
     assembly_gap    11464..11563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11703..12110)
                     /locus_tag="PLZ72_03760"
     CDS             complement(11703..12110)
                     /locus_tag="PLZ72_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35449.1"
                     /translation="MLLLDSEAISSVAHGPDLRRDRVRAHVAEMRSRDLPIETVVAVL
                     AEVVRGRLADAGVFAGLRRERVEVHPVDSRVGVRAGQLLGTVGAGSEMAIDAFLVADA
                     DLRGGAVIATCDADDLRRLAAVAERVQIVDLDR"
     gene            complement(12111..12377)
                     /locus_tag="PLZ72_03765"
     CDS             complement(12111..12377)
                     /locus_tag="PLZ72_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNA35450.1"
                     /translation="MPKEKVALTLDTEQLEELRSLVDARSLSSTVDQAVGSHLSRLRR
                     LRAVDGWLAELEAEHGPVDPETLEWAAHIVDDWASTGDRPATAG"
     gene            complement(12447..12857)
                     /locus_tag="PLZ72_03770"
     CDS             complement(12447..12857)
                     /locus_tag="PLZ72_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA35451.1"
                     /translation="MTELVLDTSVAVPLLVTSHVAHRSVSAALGDRAAALAGHALHET
                     YAVLTRLPGDARVAPADAVRLLRERFEPAALLDSRSVRSAPAVLAAAGVVGGATYDGL
                     VALAARSAGLPLATRDRRAQSTYRLLDVAVESLV"
     gene            complement(12854..13090)
                     /locus_tag="PLZ72_03775"
     CDS             complement(12854..13090)
                     /locus_tag="PLZ72_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNA35452.1"
                     /translation="MEATVDEVGRIVVPKALRDRLRLTPGTKVDVSEYGDGLHVTPVS
                     RTARLEERDGRLVAVSETPVTDDDVLALVDAGRR"
     gene            13298..13546
                     /locus_tag="PLZ72_03780"
     CDS             13298..13546
                     /locus_tag="PLZ72_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNA35453.1"
                     /translation="MRDLKNNLSRYLDRVQQGEEIIVTEHGKPVARLSAVDEATDRLA
                     ALVASGAVRPPTTRQRQRSARRIKAKGSVSDLVAEQRR"
     gene            13543..13965
                     /locus_tag="PLZ72_03785"
     CDS             13543..13965
                     /locus_tag="PLZ72_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35454.1"
                     /translation="MITYVDTSSLLKLLIDEHGSERAELIWDSADMLVSVALVVVEGR
                     AALAAAHRGALLDARQHRRASQAFEALVGALSIVEVTEALIVDAADLAEAEALRGYDA
                     LHLAAAVTVEATVFTSADSELCAAAQRRGLHVANPLSE"
     gene            complement(14143..14397)
                     /locus_tag="PLZ72_03790"
     CDS             complement(14143..14397)
                     /locus_tag="PLZ72_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35455.1"
                     /translation="MAEGPLFIVDNSEGGRNGLDYLREWSELASSIDIATGYFEIGSL
                     LDLDGHWATRRRTTWRSSPTSATTTTSRRVRRRALDRCRV"
     gene            complement(14390..15559)
                     /locus_tag="PLZ72_03795"
     CDS             complement(14390..15559)
                     /locus_tag="PLZ72_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35456.1"
                     /translation="MARNPFTPTFGVSPPLLVGRSDLLDDFVAALEDGPGAPGRMTLY
                     TGARGTGKTVMLNEVEDLARQQGWRVISETATEGLVNRLVRENLPALLSEIDPDGAKT
                     KVTGVSAPMGLGGATWATSEAHEVAPALRTQLAAACDLLAGNGAGLLLTLDEIHHQVV
                     PELREVATVLQHLVREEREIAFVGAGLPSAVSAVLNDDVLTFLRRADRHSLGPVALPE
                     VATAITEPIEHADRRIAPELAARAAEATGGYPFLIQLVGYHIWRQQPRHRDVSGDDVE
                     AGIVAARRRLGSLVHEPALADLSGVARTFLLAMAHDDGPSKMSDIASRLNVDANYASQ
                     YRLRLIASELIEPVGHGRVDFTMPYLRDYLREHVALDAQRELPPGEASSNEVERG"
     gene            complement(15603..17225)
                     /locus_tag="PLZ72_03800"
     CDS             complement(15603..17225)
                     /locus_tag="PLZ72_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNA35457.1"
                     /translation="MSGPTPAELQVLIAGLSGVAEEVGAVLQRSAFSPNIKERADCSA
                     AVFTPDGQLLAQAEHIPVHLGSMPASVSAVIGTVGDQLGPGDQAIVNDPFAGGTHLND
                     VTVVAPCFDGGRLIGWVANRAHHADLGGAAPGSLPADATEIHQEGLRMPPMRLTSEVR
                     ALLLAASRTPWERAGDLDAQVGANVVGVARLARFADAPVGAVLAHGERRCDAVLRAAP
                     QGRWRASDQLDSTGPGVDQQHPATLCCEVTIDHGDRDGPTITFDFTGTDPQRSGNTNA
                     VRAVTESAVAYVVRSVLDPTLPANAGVMRPVRVVTTEGSVVDARFPVAVGAGNVEVSQ
                     RVVDLCSRALAEVLSSRVPAASQGTMNNLLIGGPGVGFGPGGEPEPWVYYETVGGGQG
                     GRPPRPGDRSRTPLPGQSGIHTAMTNTRNTPIEALERAFSLRVLRYRLRRGSGGAGWA
                     PGGEGIERDVQVLEDATVSLITERRASQPWGLAGGEPGAVGENWLLPGGDEARAERLP
                     DKCTIQLKAGDVVRMLTPGGGGWGTPRPSNDL"
     gene            complement(17222..19033)
                     /locus_tag="PLZ72_03805"
     CDS             complement(17222..19033)
                     /locus_tag="PLZ72_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNA35458.1"
                     /translation="MRIGADSGGTFTDVVGTDGRVLKVPSTPADPGDAVRDGTAALAP
                     DGPELLAHGTTVATNALLERRGARVALYCTAGFADVIEIARQDRPSLYDPWAQRPEPL
                     VARADRLEVDERVAADGAVLVPMDIGALPPVPDGVEAVAVCLLHSDRHPGHEAAVAAA
                     LDAAGFDVSASHRVAPEFREYERTVTTVLNAYLRPICGSYLAGLDSAADDVVVMTSAG
                     GSVDLATAAELPVLLLLSGPAGGARAAAAVASACGFPDALSFDMGGTSTDVCLILDGA
                     PRAAPQQRVAGIPVRAPALDIHTIGAGGGSIARLDSGGALVVGPESAGARPGPACYGH
                     GGTRPTVTDADLVAGRIPSDVAFGDLGVLDIAAARAAIDAAGIDAEGVIAVVNAGMER
                     ALRAVSVEQGVDPAGLALVAFGGAGPLHACELAESLDVAAVIVPAAAGVLSAVGLLTA
                     PGRRELVRSWPGGVDHDGLDAALDELAARARELVAHPEPRVSTAVDCRYRGQSHELRV
                     PSVAEFPEEHRRRNGEARPGEQIEVVALRAVVETPAPATVDEVLAAWDGRWTDPVVGP
                     QVVVREDCTIWVPDGWRGEPGPLGSLVLTRVAAGVGA"
     gene            19075..19593
                     /locus_tag="PLZ72_03810"
     CDS             19075..19593
                     /locus_tag="PLZ72_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNA35459.1"
                     /translation="MTDHDHHDPHVDGSAPHVAHGVPGGSTRLPRLGSPYGRRGPSGG
                     PSVEVVDGGFIDDELTRARRSREQREQSTTAGDEPTVLRFQDYSPTESLFVPTADDRT
                     PVDPDAPTTILGVTPDADWNTIRAAHRKLLAQLHPDRFVTADERTRTDASERLARINI
                     AYHELERTRRAV"
     gene            complement(19612..20043)
                     /locus_tag="PLZ72_03815"
     CDS             complement(19612..20043)
                     /locus_tag="PLZ72_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNA35460.1"
                     /translation="MCDHCGCREYPPIAELTADHVVILELAGRLAADAEAGRHVDVEA
                     RDRLLPLLRSHAAKEELGLYALLIAQGEAEEGAYDHLEGEHREVIDAIEAGRFGHLEH
                     YALQRHIEEEEEGLFTRSTFWFDGDTWDRMTEAHRRVDAAT"
     gene            complement(20131..21153)
                     /locus_tag="PLZ72_03820"
     CDS             complement(20131..21153)
                     /locus_tag="PLZ72_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35461.1"
                     /translation="MPTSTRPVRVYTDGACRGNPGPGGWAWAVEDGGWASGHDPDTTN
                     QRMEIRAVLEAVGHLDGPLVVVSDSTYVVNCWRDRWWQGWIARGWKNSQKKPVANRDL
                     WEPLVEVFRHRPDFAMEWVKGHSGDPMNDLVDRLAVAASHGRGGSGVGAPPADLLDEP
                     DAGSGRGAGRRSNARTADGRVPAGWRLVIGGLRDPRLTADAALIGRVAEILAAQRTLH
                     PDLVVLSGLRPGAEEVGARAARSAGVPFVAVLPYPDPMAGRPERDQAAFARLLDDAAS
                     VVVLERNRPADLEGRRAALPRRDGWLRSAADAAVILTDGADPDAELAVRRFGDAVGDE
                     LWEFAL"
     gene            21228..23975
                     /gene="aceE"
                     /locus_tag="PLZ72_03825"
     CDS             21228..23975
                     /gene="aceE"
                     /locus_tag="PLZ72_03825"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNA35462.1"
                     /translation="MDGFAHQLPDIDPDETEEWLDSLDAVVDQRGEVRARYLMARLLE
                     RARERAIGTPATVSTPYVNTIPVDREPWFPGDEDLERSIRRFVRWNAAAMVVRANKHA
                     DAIGGHLSTFASSAALYEVGFHHFFRGHGAAENPASDPGDHVYMQGHASPGIYSRAFV
                     EGRLTEADLDRFRFEIDGPGRVGRGLPSYPHPRLMGDFWEYPTVSMGLGPILSIYQAR
                     FNRYLLNRRIDDTSNSRVWCFVGDGEVDEPETLGAISLAAREQLDNLTWVVNCNLQRL
                     DGPVRGNGKIIQELEAIFRGSGWNVIKVVWGSRWDELLARDVDGVLLQKMNTTVDGEF
                     QRYAIESGAYIREHFFGPDPRLRRMVEHLSDEELQKLPRGGHDYRKIYTAYAAAVRTE
                     GAPTVILAKTIKGWTLGPGVEARNATHQIKKMSAEQLVGLRDRLFLQDEISDDALADG
                     IPPYIRPAADSDEMEYLQGRRAALGGYLPARSTHTRRPLAQPSDAPFDEQLAGSGGQP
                     ASTTTAFTRLLRNLCRDGEFGSRVVPIIPDEARTFGMDALFRELKIYASQGQLYEPVD
                     HDLLLSYRESSDGQILEEGITEAGAMASWTAAATSYAHRGVPMVPFFTFYSMFGFQRI
                     GDLIWAAADARARGFLLGATAGRTTLSGEGLQHLDGHSQVLASTVPVCRAYDPAFAYE
                     TATIIRHGIDAMYPAEGADHGEDVFYYLTLYNENYPMPAMPPSPVNGVDVAAGIVEGL
                     YPWAPAPDTGGPAATILFSGPMWSVAVEAQRLLAEHYGVGAQLWSATSYQQLRVEALA
                     VERWNGLHPTETPRAPRVTQLLDRSLGPITAVTDFMRLVPDQISRWAPRRFTSLGTDG
                     FGRSDTREALRRHFEVDAAHICVTVLGQLHRDGVVDVGVVSDAIARFGVDPELPVPWH
                     G"
     gene            complement(23988..24215)
                     /locus_tag="PLZ72_03830"
     CDS             complement(23988..24215)
                     /locus_tag="PLZ72_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35463.1"
                     /translation="MESCPHCHFLMRDDAVECGVCHRPRVVVSTEGIAVVDDSRVGAG
                     RSTGVPVAIVVMLFLVLAAGTGLLVSTQSWI"
     gene            24349..24939
                     /locus_tag="PLZ72_03835"
     CDS             24349..24939
                     /locus_tag="PLZ72_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HNA35464.1"
                     /translation="MSSPAIPITGDDAADRLLEEQPLALLIGMLLDQQVPMEWAFRGP
                     ATLSERLGGRLDAARIAAMSEDDVVAVCCEKPAIHRYPAAMGRRIHSLCQDLVEHFDG
                     DAAALWSDGPTGAELYRRLRSLPGYGDEKSRIFMAILAKRLGVRPDGWEEAAAPFSDD
                     EPRSVADVDSEANLVRVREWKKMMKAARKAKSDPVD"
     gene            complement(24951..25760)
                     /locus_tag="PLZ72_03840"
     CDS             complement(24951..25760)
                     /locus_tag="PLZ72_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA35465.1"
                     /translation="MTREASSGDTSAAPVMDYTFHSGDLALSAHLAEPAVDANDAPGL
                     VLCHGFPTRGRESPQSGKSYPELAERIAAELGWVVLTMNFRGCGRAEGDFSLQGWLDD
                     IHAAVEHVAALGVTGVWICGAGTGGSLALGEGARNARVRGVAALAAQADFEDWARHPR
                     RLLLHARQVGVIKDPEFPPAFDRWSAELRAVQPLGAARDLAPRPLLVIHGDSDDLTPL
                     EDGRAIAEAHGRAELKVISGAGHELRHDPRAVAVLLGWLSRQQISDSVASS"
     gene            complement(25771..25989)
                     /locus_tag="PLZ72_03845"
     CDS             complement(25771..25989)
                     /locus_tag="PLZ72_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35466.1"
                     /translation="MDRPGKFEHHRYVGDKRTQVVYDVDELGADDEHLITELMEAETF
                     LCFGPDTLAEARNRGYRLYAPQRAASPG"
     gene            complement(26048..26494)
                     /locus_tag="PLZ72_03850"
     CDS             complement(26048..26494)
                     /locus_tag="PLZ72_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35467.1"
                     /translation="MWIWIVLAAVVCFVIAAVTIGGVTGTLATRPRRSVYDLGEAVDF
                     VADRLPEELTAEISYDDVTAVLAAHCDYLAAKGVASAKAADDIGDALVVVPDDEPVAW
                     VIGRLEEQGETIADEVVVTVLDVEEQYYEAIGAIGPRVSDSDDGGA"
     gene            complement(26532..>26834)
                     /locus_tag="PLZ72_03855"
     CDS             complement(26532..>26834)
                     /locus_tag="PLZ72_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35468.1"
                     /translation="IKHKCADMLLEVESAKSAAYYAGWCASELNDELPSVASLAKAYC
                     SEAYFHAAAENIQIHGGIGFTWEHPAHLYFKRAKSSELLFGDPTYHRELLAQRIGI"
BASE COUNT         4016 a         9794 c         9144 g         3780 t
ORIGIN      
        1 ggcccgggcg tcgatcacgt cgaggacgtc ctcgcgcgtg atgcgaccgc cgacgccggt
       61 gccggcgatg gtggccgggt ccagcccgtt ctcctcgatc agacggcgga ccagaggcga
      121 cagcagcgct cctcccgccg cggccggggc tggtgccgga gccgcgggcg ccggagcagg
      181 agccacgggc gccggagcag gagccacggg cgccggagca ggggccgggg caggagccgc
      241 gggcgccgga gcgggagcgg gttcggccgg tgcagcggga gccgcggcgg gcgccgcccc
      301 cgcgccgtcg cccagcacgg ccagaacggt gccgacgtcg acggtctcgc cctcctggac
      361 ccggatctcc gacagcacgc cgctggccgg ggccgggacc tcggtgtcga ccttgtcggt
      421 cgacacctcg aagagcggct cgtcctcggt cacggactcg ccgaccgcct tgaaccaacg
      481 ggtgatcgtt ccctcggtga cgctctctcc gagctggggc atcacgatgt cggccattgc
      541 cgttcctttc gtccggctcc tcggccggca cctctggatg cgatcgtatt caccggcgcg
      601 cgagccggtg tcagccgtgc agcgaccggc ccgtcagggc caggacggac tcgccgaaca
      661 gctcggtcag cgacgggtgg ggttggatga acgccgccac ctcgtccgcg gtcgcctccc
      721 agttgaccgc caggtagccg ccggagagct gctcggtgac ccatggcccg accatgtgca
      781 cgcccagcaa cttgcccgcc gagccgtccg gccgcttctc ggcgatgatc ttcaccaggc
      841 cgtcggtctg tccgatgatc atcgccctcg agttgccgcc gaatcggtgc ttggaggtca
      901 ccacctcgat gccggcctcc ttggccgccg cctcggacat cccggcgaag gcgacctcgg
      961 gctggcagta gatcgcccac ggcaccttgg agttgtcgac cgggacggcc tcctcgccca
     1021 ggatgtcctt gacgacgcag atcgcctcgg cgaagccgac gtgcgcgagc tgcggcgtgg
     1081 cgatcacgtc gccgatcgcc cacaccgacg gctcaccggt ccggtagttc ccgtcgacct
     1141 cgatgtggcc ccggtcggtg acccgcaccc cggtaccctc ggcgatcaca ccgtcggtga
     1201 acggcgagcg gccgaccgag accaccacca ggtcggtctc gatcgactct ccgtcaccga
     1261 agctcacggt ggtggcgccg ctcgacgacg gggcatgacc gctgaccgcg acgccggtgc
     1321 gcacgtcgat gcccttcttc ttgaaggact gaaggacgac ccgggtcacg tcctcgtcgc
     1381 agcccggcag gatcttgggc agcgcctcga ggatggtcac cgaggcgccc aggtcggcca
     1441 gggtggacgc gaactcgcag ccgatcgctc ctccgccgat gacgacggcc cgttcgggga
     1501 cgcccgggat gtggaagaac tcgtcggagg tcacgaccgg cccgcccggc tcgaagccgg
     1561 ggatgacccg gggcaccgag cccggggcga ggatgacggc gtcgccggtg agcgtgaccg
     1621 actcgtcgcc gccgacgacg gtcaccgtcc ggccggcgcc gagggtggcg gtgccgtcga
     1681 ggatcgtgac cttgcggttc ttgagcagac cggtgacgcc gccggccagc ttgtcgacca
     1741 ccgcctgctt gcgggccagc gtcgtcgacc actcgaggcc gatcggctcg accttgatgc
     1801 cgaagtcctc ggcgtgggcg atcgtgcgga ccaccgccgc ggtctcgagc agctccttgg
     1861 ccgggatgca gccccggtgc aggcacgtgc cacccacctt gtccttctcc accagggcga
     1921 tgttcagtcc ggcggacgct ccgtacagcg ccgcgccgta gcccgcgggc ccaccaccga
     1981 tgatcacgag gtcgaagtgc gtcgtctcca cgaggccgag actaccgatg ccgtcccgtc
     2041 gcccaggacg cgtcgtttga ggccgtggcg ggctcagatg atctgggcgg cctgcacggc
     2101 gaacgcgacc aggaccgcca ggccgcagac gagggcgagg atgatcagca tccgggtgct
     2161 cacgccgccg aggttagggg tggccgggac cgggggcaca gccggtagcc tcgccccgac
     2221 cccggcccgc aggaggagca gaacggtgca agacgcgctc ggtgaggtcc agtccgtact
     2281 cgtcctcggc ggcggatccg acatcggccg cgccctgtgc gtgcgcctgg cccgttcccg
     2341 ctgccggacc gtggtcctgg ccggacgtcc cgaggacgac atggagccgg tggccgacgc
     2401 ggcccgcgcc gccggggcga ccaccgtcga gctggtcgag tgggactcca ccgacgtcga
     2461 cagccaccgc aaggtgatcg atgacgtgtt cgaccgtttc gggtcgatcg acctggtgta
     2521 cgtcccggcc gggatcctcg gaagccagga ggcgttcgac gccgatccga cgttcgcggc
     2581 caaggcggtg gagatcaact tcggcggcct ggtgtcggcc tgtctggtcg tggccgaccg
     2641 cctggagaag cagggctacg gcaccctcgt gctgatgtcg tcggtcgcgg gtctccgcgc
     2701 ccgcaaggac aacttcgtct acggctccac caaggccggg ctcgacgcct tcgcccaggg
     2761 cctgggcgac tcgctgcacg gtcgcggcgt gcgggtcgtg gtcgtccgcc ccgggttcgt
     2821 gcggaccaag atgaccgagg gcatggacgc ggcgccgttc tcgaccactc cggacaaggt
     2881 cgccgagttg atcgtcgccg ggttggccaa gggcaaggag gtcgtctggg ccccgccgct
     2941 gctgcagtac ccgttcttcg tgttccgcct gctgccccgg ttcctctggc ggatcgtcgc
     3001 ggagcgagcc gggggctgag caccgcggac agctcgcgtc cggcggggcc gcgtgcctcc
     3061 gcagcgggcg ccacgtatcg tcgtgcgcca tgaccaccgc gccctccgct ccgccgaccg
     3121 ccggcgatcc gtccgccgcc acctcgtcga cacccacctc ggtgtcggcc agtcgcctga
     3181 tcggcgcgcc ggcccagcag ctgttcgacc tggtcgccga tccgaccatg cacccggtga
     3241 tcgacgggtc gggatcggtc cgacgggtca agggatcggc ccgcaagctc gccctcggcg
     3301 accgcttcac cgccaacatg cgcatcggcg tcccgtacgt catcgccaac acggtggtcg
     3361 agtacgccga gggtcggcgc atcgcctggg cgcacttcgg cggatggcgc tggcgctggg
     3421 agttcgagga gctcgagccg gtcgacggcg agcccatcac ccgggtcacc gagacctacg
     3481 actggtccac gtcccgcgcc gggtcgtcct acccgacgct gttcggacgc gaccggaaga
     3541 accaggaagc catgacccgg tcgctgaacc ggctcgccgc ctacgtcgcc cactcgggcc
     3601 agccgctctg atccgacgac ggcaccgagc accccaggac ccgcatgacc ggctcgctgg
     3661 ccgcgttcga cttcgacggc acgatcaccc gacgcgacac gctcgtctcg ttcctggccc
     3721 gggtcgccgg cccggcgcgg ttcgccggca cgtgcgcccg gctcggcctg ctcggggccc
     3781 ggggcaccat cccggtgcgc gaccgggacc gcgtcaagga gcacatgatc gagctgctcc
     3841 tgggggggac ggcagagcac gacctgcgga cccagggcga gcgctacgcc cgtgacctgc
     3901 tgaccggcga ccggctccgc cccgaggtcg tggcccggat ccacgagcac caccgggccg
     3961 gccaccgttc ggtgctcgtg tcggcgtcgc tcgtgtacta cctggagccg atcgcccggg
     4021 agctgtcgat cgacgccgtg atcggcgtcg agcccgaagt caccgacggc gtgctgaccg
     4081 gtcgactggc ccgccccaac gtccgggcgg agcagaaggc gatccgcctg cgcgagtggc
     4141 tgggcgcgcc cgccctcgga ccgatcgagg acacgaccat ccacgcctac gggaacagct
     4201 ccggtgacca cgagctgctg gcgctggccg accgtgcctg gtggctcggc cgggagtcca
     4261 agtgccccga cttcgcccgc ccgttccgcc ccggcaccgc gctctcctga cgccacccgg
     4321 acgcggggcg gcccgacccc ggagggccgg gccgcacccg agctcgctgc cgaccgctgg
     4381 ggtcagctca cttgggctgc atccgcatgc cggagtcgat gcggaccgac tcgccgttga
     4441 tgtaggagtt gcgcagcagc tccagagcga gcgacgcgta ctcggaggcc ttgcccaagc
     4501 gcttggggaa cagggtgccg gcgccgagct tctccttgaa ctgctcggcc gcctcgccct
     4561 ggccgtagat cggggtgtcg atcaggccgg ggatgatcgt gttgcagcgg atgcccaccg
     4621 cggcgaggtc ccgggcgatg gtgagggtca tgccgaccac gcccgccttg gacgccgtgt
     4681 aggacacctg accgatctgg ccctcgagcg cggcgaccga cgcggtgttg acgatggcgc
     4741 cgcgggcgcc gtcctcgtcg ggctcgttct gcgacatcgc cgtggcggca aggcggcaca
     4801 cgttgaaggt gccgatcagg ttgatctcga cgaccttgcg gaagatctcg aggtcgtgcg
     4861 ccgactcgta ggtgccgtcc cgaccgatgg tgcgggtggc ccaaccgatg ccggcggagt
     4921 tgatcaccgc gcgcagcggg cccatctcct tggcggcgtc gatcgaggcg atgacctggt
     4981 cggtgttggt cacgtcgcac tcgacgaacg cgccgccacc gatctcggcc aggatggcgt
     5041 cggccttctc cttctggtgg ccgaggtcca tgatcacgac cttggacccg gcctccgcga
     5101 gggctcggac cgtggcctca ccgaggcccg aggcgccgcc ggtgacgagt gcagatgctc
     5161 cgttgagttc cagcatggcc ggcactgtat tgaccgggtg gtcaagagcg ccagcccggg
     5221 accgggaccg gcccgccggc tcagggcgcc gcgggccccg gcccgtcgaa cacctcgtcg
     5281 acggcgcgat gggcgagctc gatctcctcc cagtcctcgg ggtcgagggc gtggaagatg
     5341 tgcgggaaca ggccgaactc ctcgtcgtcg atgtgctggg ccaggagctt caacgcggct
     5401 ccgacctcgg tcccggtcgg accgtgaccg agctgacggt gcaaggtctc gtgctcggcg
     5461 atcagctggg cgacgtcgtc gtcgagcacc ttcaggccga gcaccttggg gaacagcccg
     5521 acctcctcct tggcggcatg ccggtcgagg agcgcagtga actcctcgaa gagagcggtc
     5581 acgtcctgac gggctgccag cgcggccgac agccgggcgc tcagctcggc cagccgcacg
     5641 tgctcgtcgg ccagctccgc ggtggggccc gactcccgac agccgcagta gtcgcacatc
     5701 gcttcccaac ctacccggcg ccccaaccgg cgcgacgggc ccgagccaca tgctcaaccc
     5761 cacgggcccg ccgccgacgg gaacccatga cgacgagcgc cgatgtgtcc gcgggccagt
     5821 ccatgcggtt cgagccgacc tgggagtcgg tgtccaccca caccgtcccc tcctggtacg
     5881 acgacgccaa gctcggcgtg ttcctgcact ggggtctgta ctcggttccg gggtgggcgc
     5941 cgcaggtcga cgacatccaa cagatgctgc gcgacgaggg ccccgaggcc atgctgcgcg
     6001 ccaacccgta cgccgagtgg taccgcagct cgatgcagat cgaaggcagc ccgacgcagc
     6061 gccaccaccg agagctctac ggggacgccc cctacgacga cttcatcacc gcgttcgacg
     6121 acgggtcgcg ctcggcggac ctggacgagc tcgccggggt atgcgaacgg gccggcgccc
     6181 gctacgtggt gcttaccacc aagcaccacg agggcttcac gctgtggccc tccgcccagc
     6241 gccaccccgt caagggcgcc taccgcgccg agcgcgacat cgtcggtgag ctgaccggtg
     6301 ccgtccgcgc ccgcggcatg cgcatggggc tgtactactc gggcggctac gactggcgct
     6361 ggaacgacgc cgtcatcgcc cgccccgccg acaccacgct ggccgtgccg gccgatccgg
     6421 cctacaacgc gtacgccaca gcgcacgtcc gcgagctgat cgaccgctac gccccgtcgg
     6481 ttctgtggaa cgacgtgtgc tggcccgccg gcggcgacct ggccgctctg ttcgcgcact
     6541 actacaacac cgtcgacgac ggcgtggtca acgaccggtg gctccaaccg tcagggcccc
     6601 gaaaccgcct caccgacgcc gtcacctcgg gtctgggcga gctggtccag gccgcctggc
     6661 agcacctgcc cgagcggtgg aagtcgctga ccttccccgg cgcgaaccac tacgacttca
     6721 acactcccga gtacgcccgc ttcgacgaga tccgggacaa gaagtgggag gcgacccgcg
     6781 gggtcggcca ctccttcggc gccaaccgca acgagcgacc acgggacatc gtgaccacga
     6841 ccgaactcgt ccgcatgctc tgcgacgtgg tgtccaagaa cggcaacctc ctcatcggga
     6901 tcggcccggg ccccgacggg accgtccccg accagcaggt cgcaccgctg cggggtctcg
     6961 gcgactggtt ggcggtcaac gggccggcca tctacggcac ccggccgtgg gacgtggccg
     7021 cgaccacgac ttcggaggga accgaggtcc gcttcacctg tcgagccgat ggcgcggatg
     7081 gcgccggccc cgccgtccac gctctgctca tcgacctgcc cgggcgtcgc accttcaccc
     7141 tccgcgacgt cgacgccacc gacgtggatc atgtcgccct gctcggcctc gacgagccga
     7201 tcggctggcg ggtcaccgac gacgctcggc tcgaggtcac cctgcccgag cggctgcccg
     7261 tgaccgcggc gtacagcctg gtgctgacgg gccgtccacg cagcaccgct gccgcgacgg
     7321 actgacggcc actacggatt gacgacctcg atccgggcgc cgatcacccg gtaggtgtcg
     7381 agcgcccgct gatcatcggt ggcgaggatc gccccgtgat cgcgtgcgac ggcggcgacg
     7441 agcgcgtcgt agacggcacc gccggagatc tccccgcctt cgagctccgc caggagcccg
     7501 gccgtcgcgt ccggcgacat gaaccgcgtg tccgggaagt tcgccgccag caaccgggcg
     7561 atggccgagg ccggacgacg gttgggggcc gggagccgcg tgaggacgga gaacgattcg
     7621 aaggcggcgt ggccggccag ccccagccgg cggccgcgga ggacctcgac gctggacgaa
     7681 tggcgctcgt ggtcgccgac cgagagggcg acggcgacgc tggtgtcgac caggacgaga
     7741 tcgcgactac gactggtcgg catcgcggag ctcctgcacc tgcaccgcgg tcagcacctc
     7801 gcccgacgga gggatgacca ggaagtcgcc ctcggactcc agcgtgtcac cggtgggaac
     7861 ctcgatccgc aacgtcgcgc cgtccacgac cacgtcgacc tcccccggtc ggagaccgag
     7921 ccgatcccgc agtggcttcg ggatcaccaa tcgacctgcc ttgtcgatgg ttgcacgcat
     7981 ggcaggacga taccatggct ggtcgagact tccggcacac cggcgtttcc gagtgggtcc
     8041 gccgacgggt cggccggcaa gactcggtag cgatggtcac gaccccggat cgagccgtca
     8101 tgcggtggct gttcgcgttc agcggcgtgg tcgccgcgct catcgtgttc ggcggcttcg
     8161 tccgactgac ccgctccggt ctgtcgatcg tcgagtggaa gccggtgacc ggcgtgatgc
     8221 caccgatcgg cgagcaggca tggcacgacg cgttcgccgc ctaccgacgc agccccgagt
     8281 tcctgatcgt gaactcgtcg atgaccctcg acgagttcca gcggatcttc ctgatcgagt
     8341 ggctgcaccg cctgatcgcc cgtctggccg gcttcgcctt cctgatcccg ttcgccgtct
     8401 tcgtcgcccg ccggcggatc ccccggcgcg acctgggtcc gtacctggcc atgggctcgc
     8461 tgttcgtgct ccaggccgtc gccggatggg tcatggtggc gagcggcctg gagtcccggc
     8521 cgtcggtgag ccacatcaac ctgagcgcgc acctgctcct ggcgttcacg ctgttcgccc
     8581 tggccctgtg gaccgggctc gaccaccggt ccgcaccggt ggtcccccgg cgaggcgcgg
     8641 tgtggtcacg ctcatcgcgc ctggtcgccg gcttcgtggc cgtgctggcc gtgcagatcc
     8701 tctacggcgg gttcaccgcc ggactcaagg ccggctgggt atcgaacacg tggccactga
     8761 tgctgggtgc cgtggtgccg gagaccgcgt tcgggtcggt ggcggatctg gtcgagtcgc
     8821 cgatcaccgt catgtggatc caccgctggc tggccttcgc ggtcgcggcc atggccggcg
     8881 cggccgcgct gggtgcacgc gagcggcggg ccgacaccga cgtgcgacgc ggcaccgacg
     8941 cgatggtcgg cctggtctgc gtgcagatcc tgctggggat cgccaccgtg atgacctcgg
     9001 tgaacatggc cctggcgttg ttgcaccagg cgactgccat cgccttgttc gcctcgggcg
     9061 tgttcaccct ccatcggctc cgctccctcg acgggttcgc ctcggtcgcg cccccgctgc
     9121 atgacgcgcc cggtgacatg tccagcaccg caggtgcagc tcgtcgatga cggtgcggcc
     9181 gctgaccgcg tacggcggat cgtggtcgaa ctccaacagg ttcctggccc cgcagtccac
     9241 acgaccctgg tcgcgttccc ggaccacccg cttctgacgg gtgctcggat ggcgatgccg
     9301 gcctgacgcg tcgatcggcc gaccgtgagc gtcgtggatc aacgcccgca ggaaggatcc
     9361 cggcaccaac ctctcgagca ccgagcccgc caccggtgtg ccgtcatcga ggctgcaccc
     9421 gtcgccccgc acgtgcagca ccacctcggt cgtgacgccg aggccacccc cggtcaccaa
     9481 cccgaccaga gcatcggctc gctgctggga caccgacggc caccgctgcg cgccagcgga
     9541 cgcgtccgcg gacgcgtggg ccggtccccg gtaggccatc accgacgagt cgatcgccgc
     9601 gatcaccgcc gccgcgtccg cgggcgccaa gcggaacctg ccgagcacca tcccgtcgat
     9661 gtccgtgcgc cacgacacct cccgggcgga gcgatgacgg tcctcggtat cggccggctc
     9721 ctcgcgaccg gccagccaac gggccaactc gacgcccagc cgacctgccg ggacccgctc
     9781 cgcgatctcc aacaactcct cctcgagctc cgggctcgcg atccgggtca gctggcggac
     9841 cttgctgtag gagatccgcc ccgacgagaa cgccgcgtcg atctcgggca gcccagccag
     9901 agcccggccg attcgcagcc actcccgggc cgtgcacacc tccacgtcca gcaccgacgc
     9961 cacccagtgc gcacaggtcc gggatccgtc caggacccag tccccgctcg agtccaggtc
    10021 gcgcaccagg tgaaccaacc ggcacagagc ccgggcccat gcggagccga ccgccatcac
    10081 ttcggcacgc aggtccaccc ccggggctgg atcaccggcg ccgctgcaac gatcggcggc
    10141 gtccccgtca gccgagctcg cccccgacac agcgagcgac gtgacagcag atgatgtggc
    10201 agcagatgac gtggccgccg aggacgtggc gacggacatg gcggtctccc catgggacgg
    10261 acacccgggg catccggatt ttcacgagga ccgccttccg agcaggcagt cgagcttcgc
    10321 tcgaccagcg tagacacccg gtctgacact cccgccgatt ggcgacatcg atcgcgcgtc
    10381 cgcggacgcg cgatcgagcg gtccagcgga tctggggctc ggaggattct ctcgtcggag
    10441 atccagtccc atcggacatg gagcgcggtg cgggatgggc gcacgatgac aggggcagat
    10501 cgggtcggtc gctccggccc gtccgctgcc ccctcgaccg gtggaggtat cccatgtcgt
    10561 acggaatcgt gttggagttc gagggcgtcg gcgaagacgc ctactggtcg gccaacaagc
    10621 ggctcggcat cgacaccgac gcacggaccg gataccccga cggcctcctg gtccacgcgg
    10681 cgggaccgac cgcctcgggc tggatcgtcg tcgaggtgtg ggacgagcgg tcctcgcagg
    10741 agaggttcat ggcggagcgt ctgggtgccg ccctccaggc ggaaggacta ccggcgccgt
    10801 cgagggtcac cgactccgag ttggtcaacg cccaggcact cggctgaacc gaaccggtgc
    10861 actccggtca tcgacgcaca acagtgatcg tgcactgggg tcccggcgcg cgagccaggt
    10921 gaccatcagc agtcacgagc gggctctcga gcgcctctgc gagtgcgacg tacgtcgcgt
    10981 cgtacgcact gaggttctca cgcaactccc atacacgacc caggagacct cccaccggga
    11041 cgcgctcgat gcccagccgc tgccagacgt cgatcatccg ctcggcggcc ccgacgtcga
    11101 gatcaccccg aagcactcgg cccctgaggg cgtggacgac ctccgaatcg accaggtggg
    11161 ggcacgtggc ggcttgtgct gccaacatcg ccctcgcttc accgtcgctc atcagaccga
    11221 gcaccaccgc cgaggcgtcg atcacgatca acgggttgct cgcccagcct cgacatctgc
    11281 gacgactgca tcggcgtcga ctctgaggtc cggcagcccg gccagcagcg cagggttgtc
    11341 ggcgcgacgt gagatggaag caagctcgcg cgtggcgaac gcggagaggg acaggccctc
    11401 cctctcagcg agacgctcca agcgctcgcc gacctcgtca ggcacgttcc gcaggtacaa
    11461 ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagacg ctccaagcgc
    11581 tcgccgacct cgtcaggcac gttccgcagg tacaaggttc gcatgttgca taatgctagc
    11641 gcggcgcgca cacttcgcaa cggcattgat cacggcgaga ccccagggtt gcggcgtttc
    11701 ggtcatcgat cgagatcgac gatctgaacg cgctccgcca cggctgcaag gcgtctcaga
    11761 tcatcggcgt cgcacgtggc gatgaccgcc cctccgcgca ggtccgcgtc ggccacgagg
    11821 aaggcatcga tcgccatctc cgacccagcg ccgacggttc cgagcagctg cccggctcgc
    11881 acgcccacac ggctgtcgac agggtggacc tcgacccgct cacgtcgcag accggcgaac
    11941 acgccggcat cagccagccg accacggacc acctcggcga ggacggccac gaccgtttcg
    12001 atcggcaggt ccctcgatcg catctccgcc acgtgcgctc ggacccgatc ccgacggagg
    12061 tccggcccgt gggcgaccga cgagatggcc tcgctgtcga gcaggagcac tcaaccggcc
    12121 gtcgctggcc ggtcaccggt ggatgcccag tcgtccacga tgtgcgcggc ccactcgagc
    12181 gtctccgggt cgaccgggcc atgctctgcc tcgagctcgg cgagccaccc gtcgacagct
    12241 cggaggcgcc ggagccggga cagatgggaa ccgaccgctt ggtcgaccgt cgacgacaga
    12301 ctccgcgcat cgacgagcga gcgcagctct tcgagttgct cggtgtcgag tgtgagggcc
    12361 accttctcct tcggcatgac catatggtcg tacgttcaca cgtctactgc gaccgcaacc
    12421 agggaaccgg tgctgatctg ccggtctcag acgagcgact cgaccgccac gtccaggagc
    12481 cggtaggtgc tctgcgcacg tcggtcccgg gtggcgagcg ggaggcccgc cgaccgcgca
    12541 gcgagggcga ccaacccgtc gtaggtggcg ccgccaacga cacctgcggc agcgaggacg
    12601 gctggcgcgg aacggacgga cctgctgtcg aggagcgccg ctggctcgaa ccgctcccgc
    12661 agcagccgga ccgcgtccgc cggggcgacg cgtgcgtcgc ccggcagccg ggtgaggacc
    12721 gcgtacgtct cgtggagggc gtgcccggcg agcgcagccg cccgatctcc gagcgccgca
    12781 ctgaccgacc ggtgagcgac atgcgacgtc acgagcaacg ggacggccac gctggtgtcc
    12841 agcaccaact cggtcatcgt cggccggcgt cgaccagcgc cagcacgtcg tcatcagtga
    12901 ccggcgtctc cgagacggcc acgagccgac cgtcgcgctc ctcgagccgg gcggtacggc
    12961 tcacgggggt gacgtgaagc ccatccccgt actcgctgac atcgaccttc gtccccgggg
    13021 tgagacgcaa ccgatcccgc aaggccttgg ggacgacgat ccggccgacc tcgtcgacgg
    13081 tggcttccat gggaacacgc taccagttcg ttcccagttg acgctgcgga cgaaccgcgg
    13141 tgcaccagct gacggtcacg agccgcctcg atcgggctgt tccgcctgct ggtcagactg
    13201 cgtggacacc gctcgctggt ggatgccagg cctgaactcg ttcacaagta gctacaatgt
    13261 cttcgtgaaa gtagctacac atcgggacga ggtcggcgtg cgcgacctca agaacaacct
    13321 gagccgatac ctcgaccgcg tgcagcaagg cgaggagatc atcgtcaccg agcacgggaa
    13381 gcccgttgcc cggctgtcag ccgtcgacga agcgaccgat cggctcgcgg cactcgtggc
    13441 gtcaggcgca gtccgcccac cgacgacccg gcaacgccag cgctccgccc ggcgtatcaa
    13501 ggcgaagggc tctgtgagcg atctcgtcgc ggaacagcgg cggtgatcac ctacgtcgac
    13561 acttcctcgc tcctgaagct cctcatcgac gagcacggat ctgagcgagc tgagctgatc
    13621 tgggacagcg ccgacatgtt ggtatcggtt gcgctggtag tggtcgaggg gcgagccgcc
    13681 ctcgcagcag cccaccgcgg cgcgctcctc gacgcccgcc agcatcgaag ggccagccaa
    13741 gcgttcgagg cgctcgttgg ggcgctctcg atcgtggagg tcacggaggc gctcatcgtg
    13801 gacgcggcgg atctcgcgga ggcggaggcg ctccgtggct acgacgcgct ccacctggcc
    13861 gctgcggtca cggttgaggc gacggtgttc acgagcgccg acagcgagct gtgcgcggcg
    13921 gcgcagcggc gcggtctgca cgtcgcgaac ccgctgagcg agtagccggc atcacgaccc
    13981 gctcggcgac gggccccagc cgccgcctcc gggcgtcagc attcgcagga cgtcgccggc
    14041 cttgagctgg atcgtgcact cggctcaccc gacgacgggt ccacaccctc gatcaccgaa
    14101 cggagcgcgt cggccttgac ctccccagtc aggccgagcc gctcagaccc ggcaccgatc
    14161 cagcgcccgg cgcctcaccc gacgcgaggt agtagtcgtt gcgctcgttg gcgatgacct
    14221 ccaggtagtg cgccgccggg tcgcccagtg tccgtccagg tcgagcaggg agccgatttc
    14281 gaagtagccg gtggcgatgt cgatgctgct cgccagctcc gaccactcgc gcaggtagtc
    14341 gagcccgttc cgcccgccct cgctgttgtc gacgatgaac aacggcccct cagccacgct
    14401 cgacctcatt ggaggaggcc tcgccaggcg gcaactcgcg ttgggcgtcg agggcaacgt
    14461 gttctcggag gtagtcccgc aggtacggca tcgtgaagtc gacccttcca tgaccaaccg
    14521 gctcgatgag ctcggatgcg atgagacgca gccggtactg gctggcgtag ttggcgtcga
    14581 cgttgagcct cgaagcgatg tcgctcatct tcgaaggccc gtcgtcgtgt gccatggcga
    14641 gcaggaacgt gcgggccact ccagacaggt cggcgagagc cggctcgtgg accaacgatc
    14701 cgaggcgacg acgagcggcg acgatccccg cctcgacatc gtccccggag acgtcacgat
    14761 ggcgcggctg ctgccgccag atgtggtatc cgacgagctg gatgaggaac gggtagccac
    14821 cggtcgcctc ggcggcgcga gccgcgagtt cgggggcgat ccgccgatcg gcgtgctcga
    14881 tcggctcggt gatggccgtg gccacctcgg gaagtgcgac tggaccaagc gagtgacggt
    14941 cagctcgacg caggaacgtc aggacgtcgt cgttcagcac cgcgctcacc gcggacggca
    15001 gccccgcacc gacgaaggcg atctcgcgtt cctcgcgcac cagatgctgg agaacagttg
    15061 caacctcgcg cagctccggt accacctggt ggtggatctc gtcgagggtg aggagcaacc
    15121 cggcaccgtt gccggcgaga aggtcacatg cggcggcgag ctgggtgcga agcgccggtg
    15181 cgacctcgtg cgcttcgctg gtcgcccagg tcgctcctcc gagtcccatc ggcgcgctga
    15241 cgccggtcac cttggtcttc gcgccatccg ggtcgatctc gctcagtagc gccggcagat
    15301 tttcccggac gaggcggttc accaacccct ccgtggcggt ctcggagatg acccgccacc
    15361 cctgctggcg agcgagatcc tcgacctcgt tgagcatcac ggtcttcccg gttccacgcg
    15421 ctcccgtgta gagcgtcatc cgacccggcg cgcctgggcc gtcctcgaga gcagccacga
    15481 agtcgtccag caggtccgac cggccgacga gcagcggtgg ggagacgccg aaggtcggcg
    15541 tgaacgggtt cctggccacg ccgcgcccta ccctgccaag ccattagagg ttgttagatc
    15601 tgttagagat cgttagacgg acgaggtgtg ccccacccgc caccgcccgg cgtcagcatc
    15661 cggacgacgt cgccggcctt gagctggatc gtgcacttgt cggggaggcg ctcggctcgg
    15721 gcctcatcgc cgcctggcag cagccagttc tccccgaccg ctccgggctc accaccagcg
    15781 agaccccacg gctgcgaagc gcgtcgctcg gtgatcagcg acaccgtcgc atcttcgagc
    15841 acctggacgt cacgctcgat gccctcgccg cccggcgccc agccggcgcc accgctgcca
    15901 cgccgaagcc gatagcgcag cacccgcagt gagaacgccc gctccaacgc ttcgatgggg
    15961 gtattccgcg tatttgtcat cgcggtgtga attcccgact ggcccggtag cggtgtgcgg
    16021 ctccggtcac cgggtcgcgg gggccggcca ccctgcccgc cgccgacggt ctcgtagtag
    16081 acccacggtt cgggctcgcc accggggccg aacccaacgc cggggccacc gatgagcaga
    16141 ttgttcatcg tcccctggga cgcggcaggg acccgactcg aaagcacctc tgccagggcg
    16201 cggctgcaca ggtcgactac ccgctggctc acctcgacgt tgcccgcacc gaccgcgacc
    16261 gggaagcggg cgtcgaccac ggacccctcc gtggtcacca cccggaccgg acgcatcacc
    16321 ccagcgttgg cggggagcgt cgggtcgagg accgagcgga ccacgtacgc caccgccgac
    16381 tccgtcaccg cccgcacggc gttggtgttg ccgctccgct gggggtcggt gccggtgaag
    16441 tcgaacgtga tagtcgggcc gtcgcggtcc ccgtgatcga tcgtgacctc gcagcacaga
    16501 gtcgccggat gctgttgatc gacaccggga ccggtcgagt cgagctggtc gctcgccctc
    16561 catcggccct gcggagccgc ccgcagcacc gcatcgcagc gccgttcacc gtgggccagc
    16621 accgccccca ccggcgcgtc ggcgaaccgg gccaaccggg cgacaccgac cacgttggca
    16681 ccgacctggg cgtcgaggtc accggcgcgc tcccacggcg tccgcgacgc ggcgagcagc
    16741 agagcgcgca cctcggaggt gagccgcatc ggcggcatcc gcaggccctc ctggtggatc
    16801 tcggtcgcgt ccgcaggcag cgaacccggc gcggcaccgc cgaggtctgc atggtgcgcc
    16861 cggttggcca cccatccgat caaccggccg ccgtcgaagc acggggccac gacggtcacg
    16921 tcgttgaggt gggtgcctcc ggcgaagggg tcgttgacaa tcgcctggtc cccgggaccg
    16981 agctggtcgc cgacggtccc gatgaccgcc gacaccgacg ccggcatgga accgaggtgg
    17041 accgggatgt gctcggcctg ggccaggagc tggccgtccg gggtgaacac cgcggcggag
    17101 cagtcggcac gctccttgat gttcgggctg aacgcgctcc gttgcaggac cgcgccgacc
    17161 tcctcggcga cgccgctgag cccggcgatc agcacctgga gctccgcggg tgtcggaccg
    17221 ctcacgctcc gacccccgca gcgacccggg tcagcacgag ggagccgagc gggcccggct
    17281 ccccgcgcca cccgtcgggc acccagatcg tgcagtcctc gcggaccacc acctgcggcc
    17341 ccaccaccgg atcggtccag cgaccgtccc acgccgccag cacctcgtcg acggtggccg
    17401 gagccggggt ctccacgacc gctcgcaacg cgaccacctc gatctgctcg ccgggtcggg
    17461 cctcgccgtt gcgacgtcgg tgctcctcgg ggaactccgc caccgacggc acccgcagct
    17521 cgtggctctg gccccggtag cggcagtcga ccgcggtgct cacccggggc tccggatggg
    17581 cgacgagctc acgggcccgg gcggccagct cgtcgagcgc ggcatcgagt ccgtcgtggt
    17641 cgaccccgcc gggccaggag cgcaccagct cgcgacgccc tggagcggtg agcaggccga
    17701 cggccgagag caccccagcc gccgcgggaa cgatcaccgc ggcgacatcg agcgactcgg
    17761 cgagctcaca ggcgtgcaac ggaccggccc cgccgaaggc caccaacgcc agaccggcag
    17821 ggtcgactcc ctgttcgacc gacaccgccc gcagcgcccg ctccataccg gcgttgacga
    17881 cggcgatcac gccctcggcg tcgatcccgg cagcgtcgat cgcggcgcgg gccgccgcga
    17941 tgtcgagcac gcccaggtcg ccgaacgcca cgtcggaggg gatgcggccg gcgaccaggt
    18001 cggcgtcggt gaccgtcggg cgtgtccctc cgtggccgta acaggccggc cccggtcgag
    18061 ccccggcgga ctcggggccg acgaccagcg caccaccgga gtcgagccgg gcgatcgacc
    18121 cgccgccggc gccgatcgtg tggatgtcga gcgccggagc ccgcaccggg atccccgcga
    18181 cccgctgctg gggtgcggcg cggggcgcgc cgtcgaggat caggcagaca tcggtgctgg
    18241 tgccgcccat gtcgaagctg agcgcgtcgg ggaagccaca tgccgacgcc accgcggccg
    18301 cagcccgggc gccgccggcc ggacccgaca gcaacaggag caccggcagc tcggccgcgg
    18361 tggccaggtc gaccgatccc ccggcggagg tcatgacgac gacgtcgtcg gccgcggagt
    18421 ccaggcccgc caggtaggac ccgcagatcg ggcgcagata ggcgttgagc acggtggtga
    18481 ccgtgcgctc gtactcgcgg aactcgggtg caacccggtg cgaggcgctg acgtcgaagc
    18541 ccgcggcatc caacgcggcg gcgacggcgg cctcgtgtcc cggatgtcgg tccgagtgca
    18601 acaggcacac cgcgaccgcc tcgaccccgt cgggtaccgg gggcagggcg ccgatgtcca
    18661 tcgggaccag cacggctccg tccgcagcga cgcgctcgtc gacctcgaga cggtccgccc
    18721 gggcgacgag cggctccggc cgctgtgccc acgggtcgta cagcgacggg cggtcctgac
    18781 gggcgatctc gatcacatcg gcgaacccgg cggtgcagta cagcgccacc cgggctccgc
    18841 gacgttccag aagtgcgttg gtcgccaccg tcgtgccgtg ggccaacagc tccggcccgt
    18901 ccggtgccag ggcggccgtg ccgtcccgca ccgcatcgcc gggatcagcc ggggtcgacg
    18961 gaaccttcag tacacgaccg tcggtgccga cgacatcggt gaaggtcccg ccgctgtcgg
    19021 ctccgatccg cacgatcgga accgtagcga ccgcgccgat caccgagaga cccgatgacc
    19081 gaccacgacc accacgaccc gcacgtcgac ggatccgcgc cgcacgtcgc ccacggcgtg
    19141 cccggaggca gcacccggct gccgcgcctc ggttctccgt acggtcgacg cggcccgtcc
    19201 ggcggaccgt cggtcgaggt ggtcgacggg ggattcatcg acgacgagct cacccgtgcc
    19261 cggcgatcac gggagcagcg ggagcagtcc accacggccg gcgacgagcc cacggtcctg
    19321 cgcttccagg actactcgcc gaccgagtcg ctgttcgtgc ccaccgccga tgaccgcacc
    19381 ccggtcgacc ccgacgcccc gaccaccatt ctgggcgtga ccccggacgc cgactggaac
    19441 accatccgcg ccgcgcaccg caagttgctc gcccagctgc accccgaccg cttcgtgacc
    19501 gcggacgagc ggacgcgcac cgacgcatcc gagcggttgg cccgcatcaa catcgcctac
    19561 cacgagctcg aacggacccg ccgggcggtg tgacccgcgg ccgccggggg ttcaggtcgc
    19621 ggcgtcgacc cggcggtgcg cctcggtcat ccggtcccat gtgtcaccgt cgaaccagaa
    19681 ggtggaccgg gtgaagagac cctcttcctc ctcctcgatg tggcgctgca gcgcgtagtg
    19741 ctcgaggtgg ccgaaacgac ccgcctcgat cgcgtcgatc acctcccgat gctcgccctc
    19801 gaggtgatcg tacgcgccct cctcggcctc cccctgggcg atgagcaggg cgtacaagcc
    19861 gagctcctcc ttggccgcgt gcgaccgaag gagcggcagc agccggtcac gggcctcgac
    19921 gtcgacgtgc cggcccgcct cggcgtcagc cgccagtcgt cccgccagct ccaggatcac
    19981 gacgtgatcg gcggtcagct cggcgatggg tgggtactca cgacatccgc agtgatcgca
    20041 catgctggac cgacagtacc gccgacctac agcaccaccg acctacagca ccaccgacct
    20101 acagcaccac cgacctacag cacccccgac ctacagggcg aactcccaca gctcgtcgcc
    20161 gaccgcgtcg ccgaagcggc gcaccgccag ctcggcgtcc gggtccgcgc cgtcggtcag
    20221 gatcaccgcc gcgtcggcag ccgatcgcag ccacccgtcg cggcgtggaa gcgccgcccg
    20281 gcgcccctcg aggtccgcgg gtcggttccg ctccagcacg accaccgacg ccgcgtcgtc
    20341 gagcaggcgt gcgaacgcgg cctggtcgcg ctcgggccga ccggccatcg gatcgggata
    20401 cggcaggacg gccacgaagg gcaccccggc cgaccgtgct gctcgggcgc ccacctcctc
    20461 tgctcccggc cgcaggccgc tgagcacgac caggtcgggg tggagcgtcc gttgcgcggc
    20521 caggatctca gcgacccggc cgatcagagc ggcgtcggcg gtcagccgcg ggtcacgcaa
    20581 tccgccgatc accaggcgcc agccggcggg gacccggccg tccgcggtcc gggcgttcga
    20641 cctccggcct gctccacgac ccgatcctgc gtccggttcg tcgagcaggt ccgccggcgg
    20701 ggcgccgact cccgagccgc cgcgcccgtg ggacgcggcg acggcgaggc ggtcgaccag
    20761 gtcgttcatc gggtcacctg agtgtccctt gacccactcc atcgcgaagt cgggccgatg
    20821 ccggaacacc tcgaccagcg gttcccacag gtcgcggttg gccacgggct tcttctggga
    20881 gttcttccag ccgcgggcga tccagccctg ccaccaccgg tcacgccagc agttgacgac
    20941 gtaggtcgag tccgacacca cgaccagtgg accgtcgagg tgacctaccg cttcgagcac
    21001 ggcacggatc tccatgcgct ggttggtcgt gtcgggatcg tgacccgacg cccagcctcc
    21061 gtcctcgacg gcccaggccc acccgccggg accagggtta ccgcgacagg cgccgtcggt
    21121 gtagacgcgg acagggcgag tcgatgtcgg cacgcgccga ccctacgcgc cgacggtccg
    21181 taggctggac ccggttcctc tcccgaaacg aggtgcgcac ggtgatcatc gacgggttcg
    21241 cccaccagct ccccgacatc gaccccgacg agaccgagga gtggctcgac tcgctcgacg
    21301 cggtggtcga ccagcgcggc gaggtcaggg cccgctacct gatggcccgg ctcctcgaac
    21361 gggcccgcga gagggcgatc gggaccccgg ccacggtcag caccccctac gtcaacacga
    21421 tcccggtcga ccgcgagccg tggttccccg gcgacgagga cctcgaacga agcatccgtc
    21481 gattcgtccg ctggaacgcg gcggcgatgg tcgtgcgggc caacaagcac gccgacgcca
    21541 tcggcggcca cctgtcgacg ttcgcgagct cagcggcgct ctacgaggtg ggcttccacc
    21601 acttcttccg gggtcacggt gcggccgaga acccggcgtc ggatcccggc gaccacgtct
    21661 acatgcaggg tcacgcctcg ccgggcatct actcgcgggc gttcgtcgag ggccggttga
    21721 ccgaggcgga cctggaccgg ttccgcttcg agatcgacgg ccccggtcgg gtcggtcgcg
    21781 gtctgccctc ctacccgcac ccgcggctga tgggcgactt ctgggagtac ccgacggtgt
    21841 cgatggggtt gggaccgatc ctgtcgatct accaggcccg cttcaaccgc tacctgctca
    21901 accggcgcat cgacgacacc tccaacagcc gggtgtggtg cttcgtcggc gacggcgagg
    21961 tcgacgagcc cgagacgctg ggagccatct cgctcgcggc ccgggagcag ctcgacaacc
    22021 tgacctgggt cgtcaactgc aacctgcagc ggctcgacgg acccgtccgg ggcaacggca
    22081 agatcatcca ggagctcgag gcgatcttcc gcggcagcgg ctggaacgtg atcaaggtcg
    22141 tgtggggcag ccgctgggac gagttgctcg cacgcgacgt cgacggcgtg ctgctgcaga
    22201 agatgaacac gaccgtcgac ggcgagttcc agcgctacgc gatcgagtcc ggggcctaca
    22261 tccgcgagca cttcttcggc cccgaccccc ggctgcgccg catggtcgag cacctctccg
    22321 acgaggagct ccagaagctg ccccgtgggg gccacgacta ccgcaagatc tacacggcct
    22381 acgccgccgc ggtgcgcacc gagggcgctc cgacggtgat cctggccaag acgatcaagg
    22441 gttggacgct cggccccggc gtcgaggccc gcaacgcgac ccaccagatc aagaagatgt
    22501 ccgccgagca gctcgtcgga ctgcgcgacc ggttgttcct ccaggacgag atctccgacg
    22561 atgccctggc cgatggcatc ccgccctaca tccgacccgc cgcggactcc gacgagatgg
    22621 agtacctcca gggacgacgg gccgcgctcg gcggctacct cccggcccgt tcgacccaca
    22681 cccgccggcc gctcgcccag ccgtcggacg cgccgttcga cgagcagctc gccggctccg
    22741 gcggccaacc ggcgtcgacc accaccgcct tcacccgtct gctgcgcaac ctgtgccgcg
    22801 acggggagtt cggctcccgg gtggtgccca tcatccccga cgaggcccgc accttcggca
    22861 tggacgccct gttccgagag ctgaagatct acgcgtcgca gggtcagctg tacgagccgg
    22921 tcgaccacga cctcctgctg agctaccgcg agtcgagcga cggccagatc ctcgaggagg
    22981 ggatcaccga ggccggggcg atggccagct ggaccgcagc cgccacctcc tacgcccacc
    23041 gcggcgtgcc gatggtgccg ttcttcacct tctactcgat gttcggcttc cagcggatcg
    23101 gtgacctgat ctgggccgcg gccgacgccc gcgcccgggg attcctgctc ggcgcgaccg
    23161 ccgggcgcac gaccctgtcc ggcgagggcc tccagcacct cgacgggcac agccaggtcc
    23221 tggcgtcgac ggtgccggtg tgccgcgcct acgaccccgc gttcgcctac gagacggcga
    23281 cgatcatccg ccacgggatc gacgccatgt acccggccga gggcgccgat cacggcgagg
    23341 acgtcttcta ctacctcacc ctctacaacg agaactaccc gatgccggcc atgccgccgt
    23401 caccggtgaa cggcgtcgac gtcgccgccg gaatcgtcga ggggctgtac ccgtgggcac
    23461 ccgcgcccga caccggcggg cccgccgcga ccatcctgtt ctcgggaccg atgtggtcgg
    23521 tcgcggtcga ggcccagcga ctcctggccg agcactacgg ggtcggcgcc cagctgtggt
    23581 ccgcgaccag ctaccagcaa ctgcgggtcg aggccctggc cgtcgagcga tggaacggcc
    23641 tgcatcccac cgagaccccc cgggcaccga gggtgaccca gctcctcgac cgctccctcg
    23701 ggccgatcac cgcggtcaca gacttcatgc gactggtccc cgaccagatc tcccgctggg
    23761 ctccgaggcg gttcacgtcg ctcggcaccg acggcttcgg acgatccgac acccgcgagg
    23821 cgctgcgccg ccacttcgag gtcgacgccg ctcacatctg cgtgaccgtg ctgggacagc
    23881 tccaccgcga cggggtcgtc gacgtcggcg tggtgtccga cgccatcgcc cggttcgggg
    23941 tcgacccgga actgcccgtg ccctggcacg ggtgagtgcc cgctggctca gatccagctc
    24001 tgcgtgctga ccagcagccc cgtgccggcg gcgaggacca ggaacagcat cacgacgatg
    24061 gcgaccggca caccggtcga ccgacctgcc cccacccggg agtcgtcgac cacggcgatc
    24121 ccctcggtcg acacgaccac ccgtgggcga tggcacacgc cgcactccac ggcgtcgtcg
    24181 cgcatgagga agtggcagtg cgggcagctc tccacgtcgg gagtctcctg gtcggtcgtc
    24241 ggcttctcgt cgcacctgct gtcggttgcc gcggcccggc ccttgaacga ttcgggtcga
    24301 acgaacgggc cggaggggcc ggagggccca cagcggtaga ttggcccgat gtccagcccc
    24361 gccatcccga tcaccggtga cgacgcggcg gatcggctgc tcgaggagca acccctcgcc
    24421 ctgttgatcg ggatgctcct cgaccagcag gtcccgatgg aatgggcctt ccgcggtccc
    24481 gccaccctct cggaacgcct cggaggccgc ctcgacgcgg cccggatcgc cgccatgtcc
    24541 gaggacgacg tggtcgcggt gtgctgcgag aagccggcga tccaccgcta ccccgcggcc
    24601 atgggccggc ggatccactc cctgtgccag gacctggtcg agcacttcga cggcgacgcc
    24661 gccgcgctgt ggtccgacgg tccgaccggg gccgagctgt accgacgcct gcgctccctg
    24721 cccggctacg gcgacgagaa gtcacggatc ttcatggcga tcctcgccaa gcgcctgggg
    24781 gtgagacccg acggatggga agaggcggcg gcacccttct ccgacgacga acctcgatcg
    24841 gtggccgatg tcgattccga agcgaacctg gtccgggtcc gcgagtggaa gaagatgatg
    24901 aaggcggccc gcaaggccaa gtccgacccg gtcgactgac ccggctcagc tcagctcgac
    24961 gcgacggagt cagatatctg ctggcgcgag agccagccga ggaggacggc gacggcccgg
    25021 gggtcgtggc gcagctcgtg gccggcgccg ctgatcacct tgagctcggc ccggccgtgg
    25081 gcctcggcga tggcgcgtcc gtcctcgagc ggggtcaggt cgtcgctgtc gccgtggatc
    25141 accagcagcg gacgaggagc cagatccctc gccgcgccga ggggctgcac ggcgcgcagc
    25201 tcggcggacc agcggtcgaa cgcgggtggg aactccgggt ccttgatgac gccgacctgt
    25261 cgggcgtgca gcagcagtcg gcggggatga cgggcccagt cctcgaagtc ggcctgggcg
    25321 gccaacgccg ccactcctcg cacccgggcg ttgcgggcgc cctcccccag cgccagcgag
    25381 cctcccgtgc ccgcgccgca gatccacacg ccggtgacac cgagcgcggc gacgtgttcg
    25441 accgcggcgt ggatgtcgtc caaccagccc tgcagggaga agtcaccctc ggcacgacca
    25501 cagccccgga agttcatggt gagcacgacc caacccagct ccgctgcgat gcgctcggcc
    25561 agctcggggt acgacttgcc ggactgcggg ctctcacggc cccgcgtcgg gaacccgtgg
    25621 cacagcacga gacccggggc gtcgttggcg tcgacggccg gttcggccag atgggcgctg
    25681 agcgcgaggt cgccggagtg gaacgtgtag tccatcaccg gcgccgccga cgtgtcgccc
    25741 gatgacgcct ccctggtcac gggcgggctc tcagcccggg gatgcggcac gctgcggggc
    25801 gtacagccgg tagccccggt tgcgagcctc ggcgagggtg tcgggaccga agcacaggaa
    25861 ggtctccgcc tccatcagct ccgtgatcag gtgctcgtcg tcggcgccga gctcgtccac
    25921 gtcgtagacc acctgcgtgc gcttgtcgcc cacgtagcgg tgatgttcga acttgccggg
    25981 gcggtccatc ggttgctctc ctggctctgg aggggcgggc ggtggccatc gtaggccgca
    26041 gcgggactca ggccccgccg tcgtcggagt ccgacacccg tggtccgatc gcaccgatcg
    26101 cctcgtagta ctgctcctcg acgtcgagga cggtgaccac gacctcgtcg gcgatcgttt
    26161 cgccctgctc ctcgagccga ccgatcaccc acgccacggg ctcgtcgtcg gggacgacca
    26221 cgagggcgtc gccgatgtcg tcggccgcct tggcggaggc cacgcccttg gcagccaggt
    26281 agtcgcagtg agcggcgagc accgcggtga cgtcgtcgta ggagatctcg gcggtgagct
    26341 cctcggggag gcggtcggcg acgaagtcga ccgcctcccc gaggtcgtac acgctgcggc
    26401 gcggccgggt ggccagggtg ccggtcacgc cgccgatcgt gaccgcggcg atgacgaagc
    26461 agacgacggc ggccaggact atccagatcc acatcgtcgc cagccttgca ccccggacgg
    26521 tggccgaggg ctcagatgcc gatacgctgg gcgaggagtt cgcggtggta ggtggggtcg
    26581 ccgaacagga gctcggagct cttggcccgc ttgaagtaca ggtgggcggg gtgctcccag
    26641 gtgaagccga tcccaccgtg gatctggatg ttctcggctg cggcgtggaa gtacgcctcc
    26701 gagcagtacg ccttggccag cgaggccacc gacggcagct cgtcgttgag ctccgaggcg
    26761 caccagccgg cgtagtaggc ggcggacttg gccgactcga cctcgagcag catgtcggcg
    26821 cacttgtgct tgat
//
LOCUS       DAOQOD010000021        26226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_621_length_26226_cov_11.099232, whole genome shotgun sequence.
ACCESSION   DAOQOD010000021 DAOQOD010000000
VERSION     DAOQOD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 26226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26226
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_26226_cov_11.099232"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2286)
                     /locus_tag="PLZ72_03860"
     CDS             complement(<1..2286)
                     /locus_tag="PLZ72_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA35469.1"
                     /translation="MRRCRDEDRAANPAEQAALARWAGWGSIPQVFDDTDDRFPAERA
                     QIRQLLGTEEAWSQARRTTLNAHYTSASVVTAMWSAVRDLGVDGPVRVLEPGCGSGNF
                     LGFAPLDAVLTGVELDPTTAVIARHLYGARATIHTGAFEDLRVEDGAFDVAIGNVPFA
                     KVTPHDPHHNRGRHALHNYFLLKSLHLTRPGGLVVALTSRYTLDARNAAARREMAMLA
                     DLVGAVRLPERAFARSSGTDVVVDLLVLRRRLPGGAPTGPAWEHVVPIDLPPTSATTD
                     RGSCEPLEVNEYFDAHPDRVLGDLAAARGMYRDHELTVTPNGDLDTQLPAALQRLVAD
                     ATSTGLRFVAAPDSSAQRRPAPEVATGTFDLTHAQDGSFVTSTDGDIAQLRGGATITY
                     QPRVAKDRSELARLIGLRDAARSVLGVQLDGGSEDDLRAAQQVLTDRYDSYRRVHGPI
                     NRSSQARTGRRDPETGEELYRRVRPRMGGFREDPDWPLVAALEVFDDETQQARPAAIF
                     SERIIDPPHERHSVDSPDEAVAVCLDESGRVSLERAAELLGLDADTTRQRLGRLVYDE
                     PGTDRLIPAAEYLSGNVRTKLDAARVAALDDPRYRVNVADLEAVLPRQLEPADITARL
                     RSPWIPSRDVEYFAGEVLDATVDVEHLPQLGHWSARLRDGSRRSVALTSEWGTSRADA
                     ITLLDAALNQRLHTVTDATDDGRRVRNDAETLAARDKQEALTTRFSTWVWEDPTRAER
                     LATTYNELFSSTVLPNHDGDHL"
     gene            complement(2430..2786)
                     /locus_tag="PLZ72_03865"
     CDS             complement(2430..2786)
                     /locus_tag="PLZ72_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35470.1"
                     /translation="MNRYGRLVLEHNRTHRPDAYSQIPDPDDFFTEAGETIATAISDL
                     RDQILGQPRTNENLEAYRHRGYQAWSTAEEIVLTDHHLLQPETTETTDEDWDAPELAN
                     RYQLLAEINQIINQPL"
     gene            complement(2937..3257)
                     /locus_tag="PLZ72_03870"
     CDS             complement(2937..3257)
                     /locus_tag="PLZ72_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35471.1"
                     /translation="MKPFLRPTSRARLAPVGWTSEPAGQWAGREVEVVYDPRQHQVVL
                     CRNDPGDRTRAELAGAGYRRCASDGTQEMWVRDRSVEAAAALERETGQQHRPPAVAME
                     GRGL"
     gene            complement(3254..5110)
                     /locus_tag="PLZ72_03875"
     CDS             complement(3254..5110)
                     /locus_tag="PLZ72_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNA35472.1"
                     /translation="MFEVLVLVLAGAALLVVGVVWAGAALALLLSGAPQGVTFSAAAD
                     ATGGLPSNLAAPAAAWPAPYAEALPGAPLYWLSTGIAGAMVAAIVGLGVRWFSRSKVG
                     TTRRRPLGVDARPGFARHRDLRALLVKEPVAGRFVVARFGRWLVATEPSPLQGAQPKH
                     RFGEPRRGDRGAVALVGPSRSGKTTAAVAGILEWQGPAVLSSVKADLLGATHGWRSNV
                     GEVRIYDPMSSSIPKGTTALWSPLQQAGTVAGAQRAARALCDAAPRGGVEGGMDFWLA
                     QAEILLSGLLFIAHHGHRDMGAVCQWVFSQDRPTDAGDGEVYDAIKPLLNSNNSAVSN
                     GASDVHQSLKSVWQMEDRTRSSIYATTQTVVWPWSDPGVAASARLAHVPPGKGRRPKP
                     GKDGDGQIDLGWLLDQRRQNTVYLCSPIEDQRRLAPAFGGLLNDLINQAYRHVAETGK
                     PLDPPLLVVIDEAGNTPLRSLPEYASTLAGIGVLLVTIWQSLAQLNAAYGPQSDTILT
                     NHLTKVMYAGLSDPASLGYVDRILGQAEVDTRSRTAAERLNGGSDQYSTTQVPLAPAH
                     VLRQMRPGDALLVHGTLPPAHVRTRPFYATPHLAERAAMEPRKQRTGQEAAA"
     gene            5293..5490
                     /locus_tag="PLZ72_03880"
     CDS             5293..5490
                     /locus_tag="PLZ72_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35473.1"
                     /translation="MTTYVNGSWSGWNGSTVVKLTDGSVWRQAEYHYEYHYAYRPQAR
                     LERDELFVEGMSRGVRVRRVR"
     gene            5526..6095
                     /locus_tag="PLZ72_03885"
     CDS             5526..6095
                     /locus_tag="PLZ72_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35474.1"
                     /translation="MTRSSSNSIGREWDLAVRLAEHVGSGGEVPVLASPVLLDPSEVL
                     HADLWADGWRFLAMDVFYDEPRIVAFGGPLLFATTALSAGSARRRARAEAERLAAPQW
                     RSLGGLRILATDRRLLVWFEGAWASVWYAGIREIHPDLSARRVTILFDDDPPYALAGP
                     WVPYLVVLMTTCMIDRLGVDAVEAALLPQ"
     gene            complement(6100..7164)
                     /locus_tag="PLZ72_03890"
     CDS             complement(6100..7164)
                     /locus_tag="PLZ72_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNA35475.1"
                     /translation="MYDREALLAATDLSALADDLIGGHAGSTRSPMWTCPNPNHVQTG
                     RTPPLSIFRGHQGDQRWRCHGCGDGGSAIDLVLACKGGTVRDALEFLADRAGHHDQGT
                     DWQPSRSLAPALRPATPRCRDPEGLQRYVDECAERLWKPEGRAIRRWLTNVRAIPADV
                     LAQNRIGVDLGARYQDRPDGMPRALGAVLPTIADGRAVYAQIRVPHPDPDRPRYLNPR
                     ADLASNPKLTRMRPAERRHPEVIVTEGAIDALSAATAGYRAVGVLSATYGDESVALAL
                     ARLPHPLVLAFDADDAGNAGGDRLAALLEARDRTPVRLDLGTGDLNDAMRRSDDWPAR
                     LESAVDRAMATRSVGAGVER"
     gene            complement(7166..8473)
                     /locus_tag="PLZ72_03895"
     CDS             complement(7166..8473)
                     /locus_tag="PLZ72_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35476.1"
                     /translation="MNRASLRLNYHRGTTAHVASIYPFSVQGSFGHRGTYVGLDLLAG
                     GGEFCWDPFEAYAAGLVTNPNGWVLGEPGNGKSALVKCLLWRQAAVYGTGNAGRWIAI
                     ADPKGEYASLAEHLGLTTVRLSPGGTATINPLAPGPAAEHEPEDRQILRRAEMCTALV
                     ATVLERNLTQLEDAGVFAAVEQLTAAPLIEPTLTDVAHLVADPTDEMAERLRSHGRDL
                     AAETSNVAYALDKLLSRSLRGMFDGRSTVPLRWDGPGVVLDLSAVPLDSEALPLVMVA
                     TAGWFQQLMACPGPQRIQVLDEAWALLGNRHTAGYLQTCFKLGRTYGVANLCITHRAS
                     DLVAQADDGTATSKIAAGLLADSATKIILRQAPDQLDAAVTHFGLTEPEASIVGQLTR
                     GRALWKLGGRTAVVHHVLGPSEAAICDTDARMNARHAGEVEAA"
     gene            complement(8470..9960)
                     /locus_tag="PLZ72_03900"
     CDS             complement(8470..9960)
                     /locus_tag="PLZ72_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35477.1"
                     /translation="MRAERTDRNYKLEPLDTSGVFLGLGVVQCGLLGAGIVLGVGALT
                     VGAPLPVAAVPVVVSVAVSFTRISGHASWEWLPLLVGWLAARVRRGRRWQAPLPLWTD
                     GDDPAPMPPCLDGIDIVDIDWRAGSRLGAFRDGHRRTLTAVIPVSGPQFVVEPRGEQE
                     RLLAGWGDLLGQFAVERGVVSHLSWSDLARPSGMGDHITWLDSDQRGNPVPGAAESYR
                     ELLEDGTTTAINHEAVVTITVAKERLSRHRTSAGNADDHLRRALITSVEALLRGLRSA
                     ALSAGDPFDATGLQRLIRSRIDPVSARPRPRRGRLVERLALVRAATAGPLVLDAGWRD
                     IQVDAAHHRTWWVGTWPRLAVPPAWLEPFLSSGGITRSMTVYFQPVPTHQSRRRIERD
                     LVKLESDAIAKEDKGRRVDARHRRATQALLDREEELVAGYPEMGYAGLVTVSARTQED
                     LDEQAEVVEQLARETGMDLRVLDARQDIAWAASLPLGLAPSTLLAS"
     gene            complement(9950..11365)
                     /locus_tag="PLZ72_03905"
     CDS             complement(9950..11365)
                     /locus_tag="PLZ72_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HNA35478.1"
                     /translation="MILGIGFPNPIGWAIDKVTGFVGGVATAGFEMIIGGLVAWVVDA
                     VVWVVGGVFNFFLDSTDPNVQADWFITGDGPYATTVGIGASLLLLFVLAGIVQGTLSG
                     DVGGMLRRMGLELPISIIGMVGLVTVTQILVQLTDALSGEVLGNFQDDISDFGAVVAT
                     LSHLNGGTSTAFVVFVLGLVTVLAGLILVAELVVRSALIYIVVALAPLVFAARLWPAT
                     RGASRKLLDLLVALIVSKLVIAIALSVAAAAAVGAGSGGEVTALPEPEVFAEDPGGSV
                     TQAVGILLTAAAAFGVSAFSPLLIARLLPLTEGALVAQGVKGGPVRAGQQGMMAANTV
                     QMVSGRRYSQIANGKAAASGANGAALPAGGGAAASKAGAAGGAGGGAAASGAGPAGAV
                     AGPVAVAVVGAQAAAKGVKKGASAATGTAEAASDASDAGSSSGSGSSGPASRRPERPA
                     RPPGSGRNGQGPQRGGRPDAS"
     gene            complement(11362..12087)
                     /locus_tag="PLZ72_03910"
     CDS             complement(11362..12087)
                     /locus_tag="PLZ72_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35479.1"
                     /translation="MPSTTSPMPKRSTVIVASLATVILVAGVAVTRFSGNDSRSGHPT
                     DPQAAAEPTPRPPVAEGTPGPTLERAGLDVGFSDDEEGAVAAAVSYSTAAQRWLYFTD
                     DEIRAAVDEIATPVAAPRMADDIVLEVSMARDQLGESSGRVWWLVRPLAWNVETFDGD
                     QARVAVWVVTILSAAEVAAPQSEFLTVTVDLAWLDGDWRVDGVRDHLGPTPMTGPQDQ
                     PWDAVPFDETLAGFTRLDGEPVQ"
     gene            complement(12087..12377)
                     /locus_tag="PLZ72_03915"
     CDS             complement(12087..12377)
                     /locus_tag="PLZ72_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35480.1"
                     /translation="MTRLVGVLAQVSVDPNSTGMPGADLIQRLLNWSQMLALWGSLGA
                     LLIGAAMYGLAREGGSYGGASRGKALAMGGVVGAILAGVAPTAVNMLFEAAS"
     gene            12492..13643
                     /locus_tag="PLZ72_03920"
     CDS             12492..13643
                     /locus_tag="PLZ72_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNA35481.1"
                     /translation="MKRSKLPAAIALTFLIFLSGLVAVPFLAVAAGGAAPPCDAGGPL
                     EVEDDGTPSILGPSTLTVTDLRAWWDASGRGQPSRLGIDIADLIALYLSEGDAEGVRG
                     DMAFAQAIAETGHFTNRDTSINNFAGIAHYDNAASGSAFADPVIGVRAHIQLLKKYAA
                     GNDTALANPDVSPNAGASATTWGGLAGTWATSPTYWTLLSDVYGSMLDAAGQADADPG
                     TGTGTGMVTGCPTGDLAVAGDYALPVERRWYDEHPEWFTKPHHDYPAADIPVPTGTPL
                     YAITNGVVISTPTSGRCGIGVIINGDDGAQYTYCHGLPGSHTIDTGDRVEVGQHLMNS
                     ASTGNSTGPHLHFAIKVDGTARCPQALLVSIADGAPVTPGSLAASGCSY"
     gene            complement(13697..14395)
                     /locus_tag="PLZ72_03925"
     CDS             complement(13697..14395)
                     /locus_tag="PLZ72_03925"
                     /inference="COORDINATES: protein motif:HMM:NF021057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HNA35482.1"
                     /translation="MSEIDGFLHVTGQVVPDMGPGVAYFGTVMRGPATDASARAHVVE
                     QILDRVLPGWKVDRPAKDKDFRWLRDQASRAKAELQRSEELAERLGDNAPDMDAGDLH
                     PWAWENGRSFWNTGHFHQAVMQAAMRINAETQSKLGRMDVSETALFNEAFSLSDPKLG
                     SPRLRLAENDGSKTYESLHRGARAFAEGLYAAIRNPGMHVPTDGGEEQLALEQLAAFS
                     LLARWVDQAEVVEG"
     gene            complement(14500..17184)
                     /locus_tag="PLZ72_03930"
     CDS             complement(14500..17184)
                     /locus_tag="PLZ72_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HNA35483.1"
                     /translation="MAAPPSIAAPVIDLRAQLACDTPSWPTTEPAPTLVAAPTRTVSV
                     ERHDTYWAIAERELDDGLRWQEIQALNVGRTMVDGHVLTAADESLRSGWVLDLPAEEP
                     TVTSPEPTASEVVVVDGDNLWDMSEDRLAVDLERPPTDPEVEPYWIDVMDANRDRLVD
                     PANPSLIHPGQVLVLPPTGHEPAPPPPPPAAPVEPESPQPEPAPESEAEAPPPTTEPA
                     PTTTTTIVPTSTAPVSTVESLPQDEEAEPTAEDQPETATGSDSGLGWWLALGGLSSVA
                     LAVGAKRHIQRRRREFALEHDGADLPPPVPDQRQVHQTVVAMADEDLIAELQYALGEL
                     SVDFADSGVPCRPRLVRHSADSLEVLLDQPATVIPDGWRAEGDGLVLTLEHGINLNVD
                     LDGPSCPAPLMVTIGQPDEGAHLYLDLEVEGVLALEGDVEAARKLARSILTELALTPL
                     ADSNRVITIGDLVDPEAAGLPQLTHKETWHDFADDLTAWATGSHRALTLNNWPNAFVG
                     RGHDPNHDALMPMVVVATKPPPPELLDFLVDNQPSAVAIVVADAFEGALTTIHCDAGE
                     ICLDDLDVSFTPQQVDATALEDMGRLFNIPDPDDDPGDGVGEDHREGLDLDGSASEPP
                     TSAEHEAGAEEAAEVAEETVEPPGPPTYEILVRLLGEIRVEGAEKTMHAKATSVTAYL
                     ALNRTVTSDALEEAVWFGASGSSNRKRLLETMTRCRDAIGSQHLPPNERGSYTIGPGV
                     RTDVELFEWHVAQVQRQEPPEAIESYRSALDLVTGRPFNYTNTGRASFGWVDFEHQAT
                     TWEGRIAGVAKAFTELCLDNDQPDVAIATLRRLLQAAPLNGGVVAALMRVHIATGDRS
                     AADHLYQEHAAALDQADLGDPDDDVVQLRLDLGGGGPP"
     gene            complement(17609..18010)
                     /locus_tag="PLZ72_03935"
     CDS             complement(17609..18010)
                     /locus_tag="PLZ72_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNA35484.1"
                     /translation="MTRWNDERGSITAFVAVVATALVMVAGMAYDGGQVIHAHNAARN
                     DAEQAARAGAQEIDLTHLRQTNEPRLDPGSAETAALDYLARVGATGTALVDGADITVT
                     VTRVQPLRVLPGADRTIIVTETASAVRGDDP"
     gene            complement(18007..18441)
                     /locus_tag="PLZ72_03940"
     CDS             complement(18007..18441)
                     /locus_tag="PLZ72_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNA35485.1"
                     /translation="MKRSSLDRRSERGSVSVEVAVIAPAFVFLMLLVVFAGKVSEADG
                     NVERAAAEGARAASLRQHPGNAATDARSTVEANLATAGVSCSTLEAIVDTSDFEPGGT
                     VTVTVECTASMADVTLLGVPGTRTFTATATEVIDTYRGDDGP"
     gene            complement(18438..18794)
                     /locus_tag="PLZ72_03945"
     CDS             complement(18438..18794)
                     /locus_tag="PLZ72_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNA35486.1"
                     /translation="MTTIQVAILFPAVLIWLMLIVQYGLWWHAKQVANAAAAEAVDAA
                     QVQDGSAAQGEAAAASYLAQSGNLTGVTITVDRQATVVVAEVHGDAPQLVPGVEWAVT
                     ARSEAPVERFIPEPER"
     gene            complement(18827..19030)
                     /locus_tag="PLZ72_03950"
     CDS             complement(18827..19030)
                     /locus_tag="PLZ72_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35487.1"
                     /translation="MPDITLIFDYLCARCGIDPTDERGMTTTEVAVITFLLVGAAIVV
                     LGIIYTAAKGNADNIPTPEQPAG"
     gene            complement(19068..19982)
                     /locus_tag="PLZ72_03955"
     CDS             complement(19068..19982)
                     /locus_tag="PLZ72_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA35488.1"
                     /translation="MNVVVMCLLGAGIGAGALITWRALAPRPTPIGLVLADLARPGTP
                     IGTEAPAADPTIDRIGSISRRIIGAVGQDTSTGLLSELELVERTPERHAFDKLLGAVA
                     GLLVPNLAAVALTLAGMTMPVGVIGVLSLATAAAGFILPDFLLRDEAERRRRAFRHAL
                     SSYLDLVNVLLAGGAGIETALHAAADAGDGWGYRTIRSELRRARLTGQSPWDTFAGLA
                     DRLGVTELAELAASVSLAGSHGARIRASLAAKADTLRGHQIAETEAAAEAATERMTVP
                     VAVLLFGFLVFIAYPAIAQITSVSGPTP"
     gene            complement(19979..20842)
                     /locus_tag="PLZ72_03960"
     CDS             complement(19979..20842)
                     /locus_tag="PLZ72_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA35489.1"
                     /translation="MSVLVIAALAAGAALGLLLVAAGLTGRPILEGVGAGAQRRVGGP
                     RTARIASSVAAFIVVWAVTGWFVGGVLGALTVAALPGVLGGKSHRDREIARTEAIASW
                     TEMIRDSIVAASGLEEAIIATANVAPAPIAPEVRTLVRRLEHQPLPDALVAFGNDLNH
                     PSGDLVVAALVIAARMEASDLSSLLSRLADATRGEARMRIRVEVGRTRVRTATKVIVG
                     VVVAAVVFLALVNRDYLAVYDSPVGQLVLAVVGAIFATGGWLLTRMARIDLPERFTAR
                     AASPTTVGAEP"
     gene            complement(20839..22212)
                     /locus_tag="PLZ72_03965"
     CDS             complement(20839..22212)
                     /locus_tag="PLZ72_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNA35490.1"
                     /translation="MSATVALTPPTSSTASSAPLEGEAAELVGRLHRIVGESLTAAHS
                     AHEDEGQGRLSVPDERALARKLLADELRRLATDAYSTGRQPLDEATEDAVMTAVLDRL
                     HGLARLQPLLDDPSIRDIHISGANRVWLTMRDGTKVRGPSVAASDDELVELIATAARR
                     LGRSERRWDHAQPELNLQLPNGDRLHALMAVSGRPTVTIRRHDFDIHRVDQLVARGVC
                     NDLLAGFLSAAVRARANVIVAGGTGTGKTTMLRCLINEIDPDERLITVEDSLEIGLER
                     FEDLHPDHETLEAREANTEGVGAITLADLVRSALRMDPHRVIVGEVRGAEVLPMLLAM
                     SQGNDGSMCSIHADSSKGVFGRLAMYAAMTPERLVPEVTNLLVANAVDLIVHLGWVEG
                     ERRITSVRQVTGTLEGPHIVSNELWRPDATGTAAPAAPPTRELTDKLERYGFDIEAHA
                     MADGWWR"
     gene            complement(22209..22931)
                     /locus_tag="PLZ72_03970"
     CDS             complement(22209..22931)
                     /locus_tag="PLZ72_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35491.1"
                     /translation="MRSVALCSARGAPGVTTTALLVASHLDAVMVEADLSGGVVAVRY
                     GLGREPGLVTLAAANPHEPGGWLDHAQNAGGIPVLVGPDAAETAESLWRTAGERIMGV
                     LGRSNGTVVIDVGRIWRRPRILDAAEVALVLARPNAEELVAASHAVATLNATNLGHRG
                     AQVGVVLVGDGTYRPTEIGDSLGAPVLAHLPDDPTTAAHLRDGGTTSRSLARSRLSRA
                     VAGLVDLIDQPAAQVPDVVTVG"
     gene            complement(22928..23662)
                     /locus_tag="PLZ72_03975"
     CDS             complement(22928..23662)
                     /locus_tag="PLZ72_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35492.1"
                     /translation="MASTPTVRAPRSGAPSTNGHRPDPVAGIAPPRRRVRMPELVVGV
                     LVMTVFALGAVLWHLSAVDKSPALAVIGTVERGQTLSSSDVRVVYVASDDRLAHLTQA
                     DMNSVVGQVALVDLAPGTLLTHSVVADRPALEAGDGIVGLSLEPGGYPAMGLSPGDLV
                     NVVRTVDPADPITDGTDQGDTSDDGADASIGTGDVVIARGATVVSVEELASDRLLVSI
                     LAPEGDAVAVAARAGSGSLRLVQVSP"
     gene            complement(23664..24533)
                     /locus_tag="PLZ72_03980"
     CDS             complement(23664..24533)
                     /locus_tag="PLZ72_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35493.1"
                     /translation="MRTRVLTALLLAGASVLLAATPAGAGNRPPPGGGGGAYVDGDGD
                     PTATAGDGTANPGGGGSGGGGGGGGEGPPCEWHVVIEDDFQMGIYDVDTLQTQHSATG
                     RWLEYWCEGTGAVAVDGYFIVPEGGLVDPAALAQQALQSVQIAPPAIRTSPEESGRLY
                     VQIPTWLWIDSSWWQPYTATAQAGRVWSTVTARPVSVTWGTGDGGSEVCRGPGVAWSP
                     GVDEDASNCTHTYRRSSASADGGTFDLSATVRLEITWTSNAPFGGTLPAITRTSTLDV
                     EVGEIQAIGTRGD"
     gene            complement(24515..24928)
                     /locus_tag="PLZ72_03985"
     CDS             complement(24515..24928)
                     /locus_tag="PLZ72_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35494.1"
                     /translation="MIDRYVGYWNARFNANAGVPNPDDPALRDFATGRQLDAVVAETQ
                     SNLDSGVAFERAAEPHNIQRVTVVEIDGDRAVVQECVVDDGLVVSRDTGEVVDDSVST
                     HNVQAEMLRVDGVWKVSEARLLQQFEGVAGCALAS"
     gene            complement(25402..26100)
                     /locus_tag="PLZ72_03990"
     CDS             complement(25402..26100)
                     /locus_tag="PLZ72_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35495.1"
                     /translation="MTTIVHALNEEWATLARSPKARRALMRWTARHPELGQAQSLNDF
                     VDTRGRRDWGASALRVLATEAPGDEIAARTLLQALLGGVVRVVAGVVHDEPDSVGEVV
                     SIAWNRIRTYPPQRPGPVAANVVLDVRKEFLRNRATIECPVEPEVHNRRFGADPNPTP
                     DQVVCEQELFDELRSAQEQGMVSRAALATIIRTRLGGESLVDVAADMNMSADAIWRRR
                     TRAERCLRVLPLAS"
BASE COUNT         3862 a         8508 c         9762 g         4094 t
ORIGIN      
        1 caggtggtcg ccgtcgtggt tcgggaggac ggtcgaggag aacagctcgt tgtaggtggt
       61 ggcgaggcgt tcggcgcggg tggggtcctc ccacacccag gtcgagaacc gggtggtgag
      121 ggcttcctgc ttgtcgcggg cagcgagcgt ctcggcgtcg ttgcggacgc ggcgtccatc
      181 gtcggtggcg tcggtgacgg tgtggagccg ttggttgagc gcggcgtcga gcaacgtgat
      241 cgcatcggcg cgtgaggtgc cccattcgga ggtgagggcg acgctgcgcc ggctgccgtc
      301 gcggagtcga gcggaccagt ggccgagctg tgggaggtgt tcgacatcga ccgttgcgtc
      361 gagcacttcg ccagcgaagt actcgacgtc gcggctgggg atccacggtg agcggagtcg
      421 ggcggtgatg tcggccggtt cgagctggcg gggcaggacc gcttcgaggt ccgcgacgtt
      481 cacccggtag cgggggtcgt cgagcgcggc cacgcgggcg gcgtcgagct tggtgcgtac
      541 gttgcccgac aggtactccg cggcggggat gagccggtcg gttccgggct cgtcgtagac
      601 gaggcggccg aggcgttgcc tcgtggtgtc ggcatcgagg ccgagaagct ccgctgcccg
      661 ttcgagcgag acgcggccgg actcgtcgag gcagacggcg acggcttcgt cggggctgtc
      721 gacggagtgg cgttcatggg gcggatcgat gatccgctcg gagaagatgg cggcgggccg
      781 ggcctgttgg gtctcgtcgt cgaacacctc gagggcggcg acgaggggcc agtcggggtc
      841 ttctcggaag ccacccatgc ggggccggac cctgcggtac agctcctcgc cggtctcggg
      901 gtcacggcgc ccggtgcggg cctgactgga ccggttgatc ggcccgtgca cacgtcggta
      961 gctgtcgtag cggtcggtga gcacctgttg cgcggcgcgc aggtcgtcct cgctgcctcc
     1021 atcgagctgg acaccgagca cggagcgggc ggcgtcgcgt aggccgatca ggcgagccag
     1081 ttcggagcgg tctttggcga cgcggggctg gtaggtgatc gtggcgccac cgcggagctg
     1141 ggcgatgtcg ccgtcggtgc tggtgacgaa gctgccgtct tgggcgtggg tgaggtcgaa
     1201 ggtgccggtc gccacctccg gcgcaggtcg acgctgggcg ctgctgtccg gcgcggcgac
     1261 gaatcggaga ccggtcgagg tggcgtcggc gacaagtcgt tgaagtgcgg cggggagttg
     1321 ggtgtcgagg tcgccgttgg gagtgacggt gagctcgtgg tcgcggtaca tgcctcgtgc
     1381 cgcggccaag tcgccgagga ctcggtcggg gtgggcgtcg aagtactcgt tgacctccag
     1441 cggctcacag ctcccccggt ccgtggtcgc actggttggg gggaggtcga tgggtacgac
     1501 gtgttcccac gcggggccgg tcggcgcacc tcccggcaac cgacggcgca ggaccagcag
     1561 gtcgacgacg acatcggtgc ccgagcttcg ggcgaaggcg cgctcgggga gccggacggc
     1621 accgacgagg tcggcgagca tcgccatctc ccggcgagcg gccgcgttgc gggcgtcgag
     1681 ggtgtagcgg gaggtgaggg cgacgacgag cccgccgggt cgggtgaggt ggagcgactt
     1741 gaggaggaag tagttgtgca gggcgtggcg gccccggttg tggtgggggt cgtgcggggt
     1801 gaccttggcg aacgggacgt tgccgatcgc cacgtcgaag gcgccgtcct cgacgcggag
     1861 gtcttcgaac gctccggtgt ggatggtggc gcgggcgccg tagagatggc gggcgatcac
     1921 cgcggtggtg gggtccagct cgaccccggt gagcacagcg tcgagcggag cgaaccccag
     1981 gaagttcccc gacccacagc ccggttcgag cacccggacc ggcccgtcga cacccaggtc
     2041 acgaacggcg gaccacatgg cggtgacgac cgacgccgag gtgtagtgag cgttgagggt
     2101 ggtgcgacgg gcttggctcc acgcctcctc ggtgccgaga agctgacgga tctgcgctcg
     2161 ctcggcaggg aagcggtcgt cggtgtcgtc gaacacctgg gggatcgacc cccacccggc
     2221 ccaccgggcg agcgcagcct gttcggccgg gttcgccgca cggtcctcgt cgcggcagcg
     2281 gcgcacgatc tcgagcgcat cgaggttggc gcgcagcttg gccgtcgcac ccggtggggc
     2341 gaggtcgtcc tggctcgccg gtcggaacga ttcgcgctcg gctgaggatg cgtcgtcgcg
     2401 tgttgccgtg gccccggtcg gggtcggggc tacaggggct ggttgatgat ctggttgatc
     2461 tcggccagga gctggtaacg gttcgccagc tccggggcgt cccagtcctc gtccgtcgtc
     2521 tcggtcgtct ccggttggag gaggtggtgg tccgtcagga cgatctcctc ggcggtcgac
     2581 catgcctggt agccgcggtg ccggtaggcc tcgaggttct cgttggttcg gggttggccg
     2641 aggatctggt cgcgcaggtc gctgatcgcg gttgcgatcg tctctccggc ctcggtgaag
     2701 aagtcgtcgg ggtccgggat ctgcgaatag gcgtccgggc ggtgggtccg gttgtgctcg
     2761 aggacgagcc ggccgtagcg gttcatcgcg aatcgcctcc tttgccgtgg tgtcggcttc
     2821 ggtgggaggg agctcgatgt cgaacagcga gagctgttcg gatggagtgc ggcgcgtgct
     2881 ccgatcgctg gacacaagct accgccacga ccggccgccc cagcggagca tggtcgtcac
     2941 agcccgcggc cttccatcgc caccgcaggc gggcggtgct gctgtccggt ctcgcgttcc
     3001 agcgcggcag cggcctccac actgcggtcg cggacccaca tctcctgggt tccgtcgctg
     3061 gcgcaccgtc ggtagccggc accggccagc tcggcgcggg tccgatcgcc agggtcgttg
     3121 cggcacagca cgacctggtg ctgtcgaggg tcgtagacga cctcgacctc acgacccgcc
     3181 cactgcccgg caggttcgga cgtccaaccg accggggcca accgggcacg agacgtgggt
     3241 cgcaggaacg gcttcacgcc gccgcctcct gcccggtgcg ctgcttgcgc ggctccatgg
     3301 cggcgcgctc agccagatgc ggtgtcgcat agaacggccg agtgcggacg tgcgccggcg
     3361 gcagggttcc gtggaccagg agcgcatcgc cgggtcgcat ctggcgcagc acgtgggcgg
     3421 gcgcgagcgg gacctgggtc gtcgagtact ggtccgagcc accgttgagc cgctcggccg
     3481 cggtgcgaga ccgggtgtcg acctcagcct ggccgaggat tcggtcgacg tagccgagcg
     3541 acgccgggtc cgacagcccg gcgtacatga ccttggtgag gtggttggtg aggatcgtgt
     3601 cggactgcgg cccgtaggcg gcgttgagct gagcgaggct ctgccagatc gtcaccagca
     3661 acacgccgat gcccgcgagg gtcgacgcat actccggcag cgaccgcagc ggcgtgttgc
     3721 cggcctcgtc gatcaccacc agcagcggcg ggtcgagggg cttgccggtc tctgcgacgt
     3781 ggcggtacgc ctggttgatc aggtcgttga gcagaccgcc gaacgccggg gctagccggc
     3841 gctggtcctc gatcggcgag cacaggtaga cggtgttctg cctccgctgg tcgagcagcc
     3901 aaccgagatc gatctggccg tctccatcct tgccaggctt gggtcgacgg cccttccccg
     3961 gtgggacatg ggcgaggcga gccgaggccg cgaccccagg gtcggaccac ggccacacca
     4021 cggtctgggt ggtggcgtag atcgacgagc gggtgcggtc ctccatctgc cacaccgact
     4081 tgaggctctg gtggacatcg ctcgccccgt tcgacacggc gctgttgttc gagttgagca
     4141 acggcttgat ggcgtcgtag acctcgccgt caccggcatc ggtcggacgg tcctgggaga
     4201 acacccactg gcacaccgcg cccatgtccc ggtggccgtg atgggcgatg aacagcaggc
     4261 ccgacagcag gatctcggcc tgcgccaacc agaagtccat gccgccctcg acgccaccac
     4321 gaggcgccgc gtcgcacagc gcccgcgccg cccgctgcgc cccggcgacc gtgcccgcct
     4381 gctgcaacgg tgaccacaac gcagtcgtcc ccttcgggat tgacgacgac atcgggtcat
     4441 agatccgcac ctcgccgacg ttggatcgcc agccgtgggt cgctccgagc aggtcagcct
     4501 tcaccgatga caggaccgcc ggcccctgcc actccaagat tccggccacc gcggcagtcg
     4561 tcttgcccga ccgcgacggc ccgaccagcg cgaccgctcc tcggtcgccc cggcgcggtt
     4621 caccgaaccg atgcttcggc tgagcgcctt gcagcggcga cggctcggtg gcgacgagcc
     4681 aacgaccgaa ccgggccacc acgaaccgac ccgccaccgg ctccttcacc aacagcgccc
     4741 gcaggtcccg atgccgagcg aaccccggtc tcgcatccac cccgagcggc cgacggcggg
     4801 tcgtccccac cttcgatcga ctgaaccagc ggacaccgag gccgacaatc gccgccacca
     4861 tcgctccagc gattcccgtg ctcagccagt agagcggcgc ccccggcaac gcctcggcgt
     4921 acggcgccgg ccaggcagcg gccggtgcgg ccaggttcga cggcaggcca ccggtggcgt
     4981 cagcagccgc cgagaaggtg accccttggg gcgccccgga cagcagcagc gccagtgccg
     5041 ctcctgccca cacgaccccg acgaccagca gcgcagcccc ggccagcacg aggaccagga
     5101 cctcgaacac cccaagccca tcgcgttcca tacccaggta agccgcgaaa cgccgagaaa
     5161 accgcgctct tccctccgac cggaggtctc gaggccgccg cgaccagccc cttgatatcc
     5221 ctcggcgtcc tcggaatgtc acaccccgcg atcacactgg aggcaccgac gtcaacgact
     5281 cggaggacga ggatgacgac ctacgtgaac ggcagctggt ccggctggaa cgggagcaca
     5341 gtcgtgaagc tgaccgacgg ctccgtctgg cggcaggccg agtaccacta cgagtaccac
     5401 tacgcctacc gcccgcaggc ccgcctcgaa cgtgacgaac tgttcgttga ggggatgagc
     5461 cgcggagtcc gggtccgccg cgtcaggtag gccacttcag ggttccgcag caggaggtga
     5521 ccaacatgac tcgaagcagt agcaactcca tcggccgaga gtgggacctc gcggttcgac
     5581 tcgccgagca cgtcggttcc ggtggcgagg ttccggtcct cgccagtccg gttctcctcg
     5641 atcccagcga ggtcctccat gcggacctgt gggccgatgg gtggcggttc ctggcgatgg
     5701 acgtcttcta cgacgagcca cggatcgttg cgttcggtgg gccgcttctg ttcgccacga
     5761 cagcgctgtc agcgggttcg gcgcggcgcc gagcacgtgc cgaggcggaa cggctggcgg
     5821 caccgcagtg gcgttcgctt ggcggtctgc gaatcctcgc caccgaccgg cgactgctgg
     5881 tgtggttcga gggagcctgg gcatcggtct ggtacgcggg catccgcgag atccaccccg
     5941 acctctctgc tcgtcgggtg acgatcctct tcgacgacga cccgccctac gcccttgcgg
     6001 gaccgtgggt gccgtacctg gtcgtgttga tgacgacctg catgattgac cgtctcgggg
     6061 ttgacgcggt ggaggcagcg ctgctcccgc agtgaggggt caccgctcga ctccggcccc
     6121 gaccgagcgg gttgccatcg cccgatcgac ggcagactcg agacgggcag gccaatcgtc
     6181 agatcgacgc atcgcgtcgt tgaggtcgcc ggtgccgagg tcgagacgaa cgggggtccg
     6241 gtctcgtgct tcgagtaggg cggcgagtcg atcgcccccg gcgttgcctg cgtcgtcggc
     6301 gtcgaaggcg agaacgagcg ggtgtgggag gcgagcgagc gcgagagcga cggattcatc
     6361 gccgtacgtg gcgctgagca cgccgacggc ccggtagccg gctgtggccg cggagagcgc
     6421 gtcgatcgct ccttcggtca cgatgacctc ggggtgtcgg cgctcggcgg ggcgcatcct
     6481 ggtgagcttg gggttggagg cgaggtcggc tctcgggttg aggtaacggg gccggtcggg
     6541 atcggggtgg ggcacgcgga tctgggcgta gacggctcgg ccgtcggcga tcgtcggcaa
     6601 cacagctccg agggctcggg gcattccgtc cgggcggtcc tggtagcggg cgccgagatc
     6661 gaccccgatg cggttctggg cgaggacatc ggcggggatg gcgcggacgt tggtgagcca
     6721 ccgtcggatc gctcgtcctt caggcttcca cagccgctcg gcgcactcgt cgacgtaacg
     6781 ctggaggcct tcggggtccc gacagcgggg cgtcgccggg cggagtgcgg gcgccagcga
     6841 acgggagggc tgccagtcgg ttccctggtc gtggtgcccg gctcgatccg cgaggaactc
     6901 gagggcgtcg cgcacggtgc cgcccttgca ggcgagaacg aggtcgatgg ccgagccgcc
     6961 gtcgccgcag ccgtggcaac gccagcgttg gtcgccttgg tggccgcgga agatgctgag
     7021 cggcggggtg cggccggtct ggacgtggtt cgggttgggg caggtccaca tcgggctgcg
     7081 ggtgcttccg gcgtggccgc cgatgaggtc atcggcaagc gcggagagat cggtggcggc
     7141 gaggagggct tcgcggtcgt acatgtcacg ccgcctcgac ctcgccggcg tgcctcgcat
     7201 tcatccgggc atcggtgtcg cagatcgcgg cttcgctcgg gccgaggacg tggtggacaa
     7261 cggccgttcg cccaccgagc ttccacaggg cgcggcctcg ggtgagctgt ccgacgatgg
     7321 aggcttcggg ttcggtgagc ccgaagtgag tgaccgcggc gtcgagctgg tctggggcct
     7381 ggcgaaggat gatcttggtg gccgagtcgg ccaggagccc tgcggcgatc ttgctggtcg
     7441 cggtgccgtc gtcggcttgg gcgacgagat cggatgcccg gtgggtgatg cacaggttgg
     7501 cgacgccgta ggtgcggccg agcttgaagc acgtctggag atagccggcg gtgtggcggt
     7561 tgccgagcag cgcccatgct tcgtcgagga cctggatgcg ttgcggtccg gggcaggcca
     7621 tgagctgttg gaaccatccg gcggtcgcga ccatcaccag cgggagagcc tcggagtcga
     7681 gcggcactgc ggacaggtcg agcacgacgc cggggccgtc ccagcgaagc gggaccgtgg
     7741 aacgcccgtc gaacatgccg cgcagcgagc gggacaggag cttgtcgagg gcgtaggcga
     7801 cgttgctcgt ctcggccgcg aggtcccggc cgtgggagcg aagtcgttcg gccatctcgt
     7861 cggtggggtc ggcgacgagg tgggcgacgt cggtgagcgt cggctcgatg agtggtgcgg
     7921 cggtgagctg ttcgaccgcg gcgaacacgc cggcgtcctc gagctgggtg aggttgcgtt
     7981 cgaggacggt ggcgacgagc gcggtgcaca tctcggcgcg tcgcaggatc tgacggtctt
     8041 cgggctcgtg ttcggccgcc ggacccggag cgagcgggtt gatggtggcg gtgccgccgg
     8101 ggctgagccg cacggtggtg agcccgaggt gctcagcgag cgacgcgtac tcgcctttcg
     8161 gatcggcgat tgcgatccag cgtccggcgt tgccggtgcc gtagacggcg gcttggcgcc
     8221 acaggaggca cttcacgagg gcggacttgc cgttgccggg ttcgccgagg acccagccgt
     8281 tggggttggt gaccagcccg gcggcgtaag cctcgaaggg gtcccagcag aactcgcctc
     8341 ccccggcgag caggtcgagg ccgacgtagg tgcctcggtg tccgaacgag ccctggaccg
     8401 agaacgggta gatgctggcc acgtgcgcgg tggttccgcg gtggtagttg agtcgaagcg
     8461 atgcgcggtt catgacgcca gcaacgtgga gggggcgaga ccgaggggca gcgacgcggc
     8521 ccacgcgatg tcttgacggg cgtcgagcac ccgcaggtcc atgccggtct cacgggcgag
     8581 ctgctcgacg acctcggcct gttcgtcgag gtcttcttgg gttcgggccg acacggtgac
     8641 aagcccggcg tatcccatct cggggtagcc ggcgacgagc tcctcctcgc ggtcgagcag
     8701 tgcttgggtg gctcgccggt ggcgggcgtc gacccgtcga cccttgtcct ccttggcgat
     8761 ggcgtcggac tcgagcttca ccaggtctcg ttcgatgcgg cggcggctct gatgggtcgg
     8821 gaccggctgg aagtagacgg tcatcgaccg ggtgatcccg ccgctggaca ggaacggttc
     8881 gagccatgcc ggggggacag cgagtcgggg ccacgtgccg acccaccagg tgcggtgatg
     8941 cgcggcgtcg acctggatgt cccgccatcc ggcgtcgaga acgagtgggc ctgccgtggc
     9001 agcgcggacg agggcgagac gttccacgag acgtcctcgt ctcggtcgcg gtcgggcgct
     9061 gaccgggtcg atgcgagaac ggatcagccg ctggagaccc gtcgcgtcga acgggtcacc
     9121 ggcgctgagc gccgctgagc gcagaccacg gagcagcgct tcgacggacg tgatgagcgc
     9181 tcgacggagg tggtcgtcgg cgttgccagc gctggtgcgg tgacgggaga ggcgttcctt
     9241 cgcaacggtg atggtgacga cggcttcgtg gttgatcgcc gtggtcgtgc cgtcctcgag
     9301 cagctctcgg tacgactcag cggcgcccgg cacggggttg ccgcgctgat cgctgtcgag
     9361 ccaggtgatg tggtcgccca tgcccgacgg acgggcgagg tccgaccacg agaggtgcga
     9421 gacgacgcct cgctcgaccg cgaactgtcc gagcaggtcg ccccaaccag cgaggagtcg
     9481 ttcctgttca cccctcggtt cgacgacgaa ctgtggaccc gacaccggga tcaccgcggt
     9541 gagggttcgg cggtgaccgt cgcggaaggc accgaggcgg cttccagccc gccagtcgat
     9601 gtcgacgatg tcgatgccgt cgaggcacgg tggcatcggc gcgggatcat ccccgtcggt
     9661 ccacaacggc agcggtgcct gccagcgccg accgcgtcgc acacgggccg cgagccagcc
     9721 cacgagcagg ggcagccact cccagctggc gtgaccgctg atgcgggtga agctcaccgc
     9781 gacggacacc acaaccggca ccgctgcgac cggcagtgga gcgccgacgg tcagcgcacc
     9841 cacaccgagg acgatccctg cgccgaggag cccgcactgc acaacgccga ggccgaggaa
     9901 cacgccggag gtgtcgagcg gttcgagctt gtagttgcgg tcggtgcgtt cagctcgcat
     9961 cgggacgacc tcctcgctgt ggcccttgac cgttgcggcc cgaccccggc ggcctggccg
    10021 gtcgttcggg gcgtctcgac gccggaccag aggaccccga tccggagctg gaccctgcgt
    10081 cgctggcgtc ggacgcggct tccgcggtcc cagttgccgc tgacgcgccc ttcttgaccc
    10141 ccttggctgc cgcctgggca ccgacgaccg cgacggctac gggcccagcc actgcaccag
    10201 cagggccggc tccgctcgct gcggcacctc cacccgcgcc tccggccgcg ccagccttgg
    10261 atgcagccgc accaccaccc gccgggagtg cggcaccgtt ggcgcccgac gcagcagcct
    10321 tgccgttggc gatctggctg tagcgccgac ccgacaccat ctggacggtg ttggcggcca
    10381 tcatcccctg ttgcccagcg cggacgggtc cgcccttcac gccttgagcg acgagcgcgc
    10441 cctcggtgag cggtaggagc cgggcgatca gcaacggcga gaaggcggag acaccgaatg
    10501 ccgccgcggc ggtgagcaat atgccaacgg cctgggtgac cgacccgccg gggtcctcgg
    10561 cgaacacctc cggttcgggc aacgccgtga cctcgccacc cgaaccggcc ccgaccgcgg
    10621 ccgcagctgc gacggacagc gcgatggcga tgacgagctt cgacacgatc agtgcgacga
    10681 gcaggtcaag gagcttgcgg ctggcgccgc gggtggcggg ccaaagtcgg gcggcaaaca
    10741 cgagcggggc gagggcgacg acgatgtaga tcagcgcgga gcgcacgacg agctcggcca
    10801 cgaggatcaa cccggcgagc acagtgacca gcccgaggac gaagaccacg aacgcggtag
    10861 acgtgccgcc gttgaggtgg ctgagggtgg cgaccacggc tccgaaatcg gagatgtcgt
    10921 cttggaagtt gccgaggacc tcgccggaga gcgcgtcggt gagctggaca aggatctgcg
    10981 tgacggtgac gagcccgacc atcccgatga tcgagatcgg cagctcgagg cccatgcggc
    11041 gcagcatccc gccgacgtct ccgctgaggg ttccctggac gatcccggcc aggacgaaca
    11101 acaacaggag gctggcaccg atcccgacgg tggtggcgta ggggccgtca ccggtgatga
    11161 accagtcggc ctggacgttg gggtccgtgg agtccaagaa gaagttgaac accccgccga
    11221 caacccaaac cacggcgtcg accacccagg cgaccagccc accgatgatc atctcgaacc
    11281 ctgcggtggc gacaccgccg acgaacccgg tgaccttgtc gatggcccaa ccgatcggat
    11341 tggggaatcc gatgccgagg atcactggac cggctccccg tcgagtcggg tgaacccggc
    11401 gagggtctcg tcgaagggga cggcatccca cggctggtcc tgcggtccgg tcatcggggt
    11461 cggaccgagg tggtcacgca caccgtcgac gcgccagtcg ccgtcgagcc acgcaaggtc
    11521 gacggtgacg gtcaggaact cgctctgcgg ggcggcgacc tccgcggccg acaggatcgt
    11581 cacgacccac accgccacgc gggcttggtc gccgtcgaag gtctcgacgt tccaggccag
    11641 gggccgcacc agccaccaga cccggcccga cgattcgccg agctggtcgc gggccatcga
    11701 gacctcgagg acgatgtcgt cggccatgcg tggcgcagcg accggggtgg cgatctcgtc
    11761 gaccgcggca cggatctcgt cgtcggtgaa gtacagccag cgctgcgccg ccgtcgagta
    11821 cgacaccgcg gcggcgaccg ctccctcttc gtcatcggag aacccgacat cgaggccagc
    11881 tcgttccagc gtcggtccgg gggttccctc ggcgaccggt ggccgaggtg tcggctcggc
    11941 ggcggcctga gggtcggtcg gatggccgga ccgactgtcg ttgccggaga agcgggtgac
    12001 cgcgacgccg gccacgagta tgaccgtggc cagtgacgcc acgatgacgg tgctgcgctt
    12061 cggcatcggg gaggtggtgg acggcatcag ctcgccgcct cgaacagcat gttcaccgcg
    12121 gtcggggcga ctccggcgag gatggcgccg acgacgccac ccatggcgag cgccttgcct
    12181 cggctcgcac cgccgtagct gccgccctca cgtgcgaggc cgtacatggc ggcgccgatg
    12241 agcagagcgc cgaggctgcc ccacagggcg agcatctggg accagttgag cagcctctgg
    12301 atcaggtcgg ccccgggcat gcccgtggag ttcgggtcga cagacacctg ggcgagaact
    12361 ccgacgagac gtgtcatcgg gccacctcct tcgagtcggg gaaacgagcg gcacaggggt
    12421 aagccgcgaa ccggcccgaa aatcgacaag aaatcccgcg ggaacctcgg cctgtcgcgg
    12481 cctactcagg tatgaagcgc tcgaagctcc ccgccgccat cgccctcacg ttcctgatct
    12541 tcctcagcgg actcgtagct gtgccgttcc tcgcggtcgc cgccgggggc gcggcgccac
    12601 cgtgcgacgc cgggggtccg ctcgaagtcg aagacgacgg gacgccaagc atcctcggac
    12661 cctccacgct caccgtcacc gacctccggg cttggtggga tgccagcggc cgaggtcaac
    12721 cgagccgtct cggcatcgac atcgcggatc tgatcgccct gtacctgtcg gagggcgatg
    12781 ccgaaggtgt ccgcggtgac atggccttcg cgcaggccat tgccgagacc ggccacttca
    12841 ccaaccgcga cacgtcgatc aacaacttcg ccggcatcgc ccactacgac aacgctgcgt
    12901 ccggcagcgc gttcgccgac cccgtcatcg gcgtacgagc ccacatccag ctcctcaaga
    12961 agtacgcagc aggcaacgac acggcgctcg ccaaccccga cgtgtccccc aacgccgggg
    13021 cctcggccac cacctggggt ggactcgccg ggacctgggc gacctcgccc acctactgga
    13081 ccctgctcag cgacgtctac ggctccatgc tcgacgctgc cggacaggcc gacgccgacc
    13141 ccggcaccgg caccggcacc ggcatggtca ccggatgccc gaccggcgac ctcgcggtgg
    13201 ccggcgacta cgccctaccg gtcgaacgcc gctggtacga cgagcacccc gagtggttca
    13261 ccaagcccca ccacgactac cccgccgccg acatccccgt ccccactggc accccgctct
    13321 acgccatcac caacggcgtc gtgatctcga cgcctaccag cggccgttgc ggcatcgggg
    13381 tcatcatcaa cggcgacgac ggagcccagt acacctactg ccacggcctc cccggcagtc
    13441 acaccatcga caccggcgac cgggtcgagg tcggccagca cctcatgaac agcgcctcca
    13501 ccggcaacag caccggcccc cacctccact tcgccatcaa ggtcgacggc accgcgaggt
    13561 gcccccaggc gcttctggtg agcatcgccg acggagcgcc cgtcacccca ggttccttgg
    13621 cagcgagcgg gtgctcgtac tgagacaccc cgttcgggcc cgcgagggac cgctgcgaag
    13681 tgaatcgatc gtcaggtcag ccttcaacaa cctcggcctg atcaacccac ctggcgagca
    13741 ggctgaaggc agcgagctgt tcaagtgcga gttgctcctc gccgccgtcg gtcggcacat
    13801 gcattcccgg attgcggata gcggcgtaca ggccttcggc gaatgctcgt gcaccgcggt
    13861 gcaggctctc gtaggtcttg cttccgtcgt tctcggcgag ccgcagccga ggagacccca
    13921 gcttcggatc gctcaacgag aacgcctcgt tgaacaaggc cgtctcggag acatccatac
    13981 ggccaagctt cgactgggtc tcggcgttga tccgcatggc cgcttgcatg acggcttgat
    14041 ggaagtgtcc cgtgttccag aacgaccggc cgttctccca tgcccacgga tgcagatcgc
    14101 ccgcgtccat gtctggcgcg ttgtccccca gcctctcggc cagctcctcc gagcgctgca
    14161 actccgcctt ggctcgtgat gcctggtccc gcagccatcg gaagtccttg tcctttgcag
    14221 ggcgatcaac cttccaccca ggtagcacgc ggtccagtat ctgctcgacg acgtgcgccc
    14281 gggccgaagc atccgttgcc gggccgcgca tgaccgtgcc gaagtacgcc actccaggcc
    14341 ccatatcggg gactacctga ccagtgacgt gcaagaaccc gtcgatctcg ctcaccgccc
    14401 acccggtatc cagacctgcc actcatccat cctcgcagcc cgtggtggct caaggatgtt
    14461 cctcggcctg acagaccgac ggaccccaga ggccccacat cagggtggac cgcccccacc
    14521 gaggtcgaga cgtagctgaa cgacatcgtc gtcggggtcg ccgaggtcgg cttggtcgag
    14581 ggcggctgcg tgctcttggt agaggtggtc ggcggctgag cggtcgccag tcgcgatgtg
    14641 gactcgcatg agcgcggcga cgacaccgcc gttgagcggt gcggcctgga ggaggcggcg
    14701 gagggtggcg atggcaacgt cgggctggtc gttgtcgagg cacagctcgg tgaacgcctt
    14761 ggcgacccct gctatgcggc cttcccaggt ggtggcctgg tgctcgaagt cgacccagcc
    14821 gaacgaggcg cggccggtgt tcgtgtagtt gaacggtcgg ccggtcacca ggtcgagcgc
    14881 ggatcggtag ctctcgatcg cctcgggtgg ctcctggcgc tgcacctgtg caacgtgcca
    14941 ctcgaagagc tcgacatcgg tccgcacgcc gggaccgatc gtgtaggagc cccgctcgtt
    15001 gggtgggagg tgctgggagc cgatggcgtc ccggcagcgg gtcatggtct cgaggaggcg
    15061 tttgcggttc gacgaccccg acgcgccgaa ccagaccgcc tcctcgagag cgtcggacgt
    15121 cacggttcgg ttgagcgcca ggtaggcggt gaccgaggtg gccttggcgt gcatggtctt
    15181 ctcagcgccc tccacgcgga tctcgcccag gagccggacc aggatctcgt aggtgggtgg
    15241 gccaggcggc tcgaccgtct cttcggccac ctctgcggcc tcctcggctc cggcctcgtg
    15301 ttcggcagac gtgggtggtt cgctggccga tccgtcgagg tcgagacctt cgcggtgatc
    15361 ctcgccgacg ccatcgccgg ggtcgtcatc ggggtcgggg atgttgaaca ggcgacccat
    15421 gtcctcaagg gcggtggcgt cgacctgctg tggggtgaag gagacgtcaa ggtcgtcgag
    15481 gcagatctcc ccggcgtcgc agtggatggt ggtcagggcg ccctcgaatg catcggcgac
    15541 gacgatggcg acagcggagg gctggttgtc gacgaggaag tcgaggagct cgggtggggg
    15601 tggcttggtg gcaacgacga ccatcggcat cagcgcgtcg tggttcggat cgtgtccacg
    15661 gccgacgaag gcgttgggcc agttgttcaa ggtgagggcg cggtgcgaac cggtcgccca
    15721 tgcggtgagg tcatcggcga agtcgtgcca agtctccttg tgcgtgagct ggggtaggcc
    15781 cgctgcttcg gggtcgacga ggtcgccgat ggtgatgacc cggttgctgt cggccagcgg
    15841 ggtcagggcg agttcggtga ggatcgaccg ggcgagtttg cgggcggctt cgacgtctcc
    15901 ttcgagtgcg aggacgcctt cgacctcgag gtcgaggtag aggtgggcgc cctcgtctgg
    15961 ttggccgatg gtgaccatga gcggtgcagg gcaggatggg ccatcgagat cgacgttgag
    16021 gttgatgccg tgctcaaggg tgagcacgag cccgtcacct tcagctctcc agccgtcggg
    16081 gatcaccgtt gccggctggt cgaggagcac ctcgaggctg tcggcgctgt ggcgaacaag
    16141 gcgagggcgg caaggcactc cgctgtcggc gaagtcaacg gacagctcgc ccagcgcgta
    16201 ctgaagttcg gcgatgaggt cctcgtcggc catggccacc acggtctggt ggacctgtcg
    16261 ttggtcgggc acgggcgggg gtagatctgc gccgtcgtgc tcgagcgcga actcgcgccg
    16321 tcggcgctgg atgtgccgct tggcgccaac ggcgagagcc acgctcgaca gtccgccgag
    16381 ggcaagccac cagcccaacc cggagtccga ccccgtcgcc gtctctggct ggtcctcggc
    16441 ggtcggctca gcctcctcat cctgcgggag tgactcgacc gtcgagaccg gggctgtgga
    16501 cgtcggcacg atcgtggtcg tggtggtcgg cgccggttcg gtggtaggcg gtggtgcttc
    16561 ggcttcggac tcgggggctg gctcgggctg cggggactcc ggctcgacgg gagcggcagg
    16621 aggtggcggc gggggtgcag gttcgtggcc ggtcggcgga aggacgagga cctggcctgg
    16681 gtggatgagg ctggggttgg cggggtcgac gaggcggtcg cggttcgcgt ccatgacgtc
    16741 gatccagtac ggctcgacct cggggtcggt tggtgggcgt tcgaggtcga cggcgaggcg
    16801 gtcctcggac atgtcccaca ggttgtcgcc atcgacgacc acgacttcgc tggctgtggg
    16861 ttccggggag gtgacggttg gttcttcggc ggggaggtcg agaacccatc cggaccgaag
    16921 gctctcgtct gcggcggtga ggacgtgccc gtcgaccatg gtgcgcccga cgttgagtgc
    16981 ctggatctct tgccagcgca gcccgtcgtc gagctctcgt tcagcgatgg cccagtaggt
    17041 gtcgtggcgt tcgaccgaaa cggttcgagt cggcgccgcc accaacgtcg gcgcaggctc
    17101 ggtcgtgggc cacgaaggcg tgtcgcatgc gagctgggcg cgcaggtcga tgaccggcgc
    17161 ggcgatggac ggcggcgcgg ccactgcggc ggactcgacg gtggcgacgg ccggtgttgg
    17221 cggtggtgga gctgcgacag ctcgagctgg ttggagcggg cgacgagcat gacgatgcct
    17281 gcgacgaggc gggccgcggc tgcttggagt ccgggggcga tggggaggtc gcggggttgg
    17341 cgccccttga acgcggcggt ggcctcgatg aggaacgcca aggcgagctg cgcccaggcg
    17401 atccatgcga cgacggcgag ggcgttgacg atgaaggtgt cggtgatgcc ggtgttgatg
    17461 gcggtggaga gggtgtcgct atcggggatc gtggtcggta gcggccaacc gacgagtgtg
    17521 gccaggccga tcgggacacc gatgagaagg agggcggcga ccgcgaggga gcccaggcct
    17581 cgaaggaccg cggtcgatcg gcggctggtc atgggtcgtc tcctcggacg gcggaggcgg
    17641 tttcggtgac gatgatggtc cggtcggcgc cggggaggac gcgcaggggc tggacgcgag
    17701 tgacggtcac ggtgatgtcg gcgccgtcga cgagcgccgt accggtggca ccgacgcggg
    17761 cgaggtagtc gagcgcggca gtctcagcgg agccggggtc gaggcggggt tcgttggtct
    17821 ggcggaggtg ggtgaggtcg atctcttgcg cgccggcgcg ggctgcttgt tcggcgtcgt
    17881 tgcgcgccgc gttgtgggcg tggatgactt ggcctccgtc gtaggccatg ccggcgacca
    17941 tgacgagtgc ggtggcgacg accgcgacga acgcggtgat gctgcctcgt tcgtcgttcc
    18001 atcgggtcac gggccgtcgt ctcctcggta ggtgtcgatc acttcggtgg ccgtcgcggt
    18061 gaaggtccgg gttcccggga cgccgaggag ggtcacgtct gccatcgacg cggtgcattc
    18121 gacggtgacg gtgacggtgc cgccgggttc gaagtcgctg gtgtcgacga tggcttcgag
    18181 cgtcgagcag gacacgcctg cggtagcgag gttggcctcg acggtggatc gggcatcggt
    18241 tgcggcgttg ccggggtgtt ggcgcagtga tgcggcgcgt gctccctcgg ctgcggctcg
    18301 ctcgacgttg ccgtcggcct cggagacctt gccggcgaac acgacgagca gcatgaggaa
    18361 cacgaacgcg ggggcgatga cggcgacctc gaccgatacc gagccacgct cgctgcgccg
    18421 gtcgagcgat gagcgcttca tcgttcgggc tccgggatga accgttcgac gggggcttcg
    18481 ctgcgggcgg tgaccgccca ttcgacgcca gggaccagtt gcggtgcgtc gccgtggact
    18541 tcggcgacga cgacggtggc ctggcggtcg acggtgatgg tgacgccggt gaggttgccg
    18601 gactgggcga ggtagctggc cgcagcggct tcgccctggg cggctgagcc gtcttggacc
    18661 tgggcggcat cgacggcttc ggccgcggcg gcgttggcga cctgcttggc gtgccaccac
    18721 agcccgtact ggacgatgag catgagccag atcaggacag ccgggaacaa gatggcgacc
    18781 tggatggtgg tcatcccccg ctcgtcacgc agccgggcca tggtcctcag ccggcgggct
    18841 gctcaggggt cgggatgttg tcggcattgc ccttggcggc ggtgtagatg atcccgagga
    18901 caacgatggc ggcgccgacg agcaggaagg tgatgaccgc gacctcggtc gtggtcatgc
    18961 cccgttcgtc ggtggggtcg atgccgcagc gggcgcagag gtagtcgaat atcagggtga
    19021 tgtcgggcat cgggttgtcc tttctggtgg gtgcaggggg aactcgctca gggggttggc
    19081 ccgctcaccg aggtgatctg tgcgatggct gggtaggcga tgaagacgag gaacccgaac
    19141 agcaggacgg cgacggggac ggtcatgcgt tcggtggctg cttcggcggc ggcttcggtc
    19201 tcggcgatct ggtggcctcg gagggtgtcg gccttggctg ccagtgatgc ccggatgcgg
    19261 gcgccgtggg atccggcgag ggacacgctg gctgcgagtt cggcgagttc ggtcacgccg
    19321 agccgatcgg cgagtccggc gaaggtgtcc catggggatt ggccggtgag gcgtgctcgg
    19381 cgtagttcgc tgcggatggt gcggtagccc cacccgtctc cggcgtcggc ggcagcgtgc
    19441 agggcggtct cgatgccagc tcctccggcg agcaggacgt tgaccaggtc caggtaggac
    19501 gacagggcgt gacggaacgc tcgtcggcgg cgttcggcct cgtcgcgcag aaggaagtcg
    19561 gggaggatga acccggcggc ggcggtggcg agggacagga cgccgatgac accgacgggc
    19621 atggtcatcc cggcgagggt gagggcgacc gccgcgaggt tggggacgag gagcccggcg
    19681 acggcgccga ggagcttgtc gaaggcgtgg cgttcggggg tgcgttcgac cagctcgagc
    19741 tcgctgagca gccctgtcga ggtgtcctgt ccgacggccc cgatgatccg ccgcgagatc
    19801 gacccgatgc ggtcgatggt cgggtccgct gctggcgcct cggtaccgat cggtgttcct
    19861 gggcgggcca ggtcggcgag gacgagcccg atcggggtgg gtcgaggggc gagcgcacgc
    19921 catgtgatca gcgcgccggc gccgattccc gcgccgagga ggcacatgac gacgacgttc
    19981 acggctcggc tcccaccgtt gtcgggctgg cggctcgagc cgtgaagcgc tcgggtaggt
    20041 cgatgcgggc catgcgggtg aggagccatc cgccggtggc gaagatggcg ccgacgacgg
    20101 ccagaacgag ttgaccaacg gggctgtcgt agacggcgag atagtcccgg ttgaccagag
    20161 cgaggaacac cactgcggcg acgacgacac cgacgatcac cttggtggca gttcgcactc
    20221 ttgtacgacc gacctcgacg cggatgcgca tcctcgcctc gccgcgggtg gcatcggcga
    20281 gccgggacag gaggctggac aggtcgctgg cttccatccg ggcggcgatg acgagcgctg
    20341 cgacgaccag atcaccggag gggtggttga ggtcgttgcc gaacgcgacg agcgcatcgg
    20401 gcagcggttg gtgttcgagg cgacggacga gggtgcggac ctcgggggcg atcggtgcag
    20461 gggcgacgtt cgcggtggcg atgatggctt cttcgagccc ggacgcggcg acgatcgagt
    20521 cgcggatcat ctcggtccac gaggcgatgg cctcggtgcg ggcgatctca cggtcgcggt
    20581 ggctctttcc tccgagcaca cccggcaggg cggcgacggt gagcgcgccg aggaccccgc
    20641 cgacgaacca gccggtgacc gcccacacaa cgatgaacgc agcgacgctg gacgcgatgc
    20701 gggccgttct ggggccgccg actctgcgtt gtgcgccggc tcctacccct tcgaggatcg
    20761 gtctgccggt gagcccggcg gcgacgagca ggagccccag cgcagcgccg gccgcgagtg
    20821 cggcgatgac gaggacgctc atcgccacca cccgtcggcc atggcgtggg cttcgatgtc
    20881 gaagccgtag cgctcgagct tgtcggtcag ctcccgggtg ggtggcgcgg ccggggccgc
    20941 cgtgcccgtg gcgtcgggac gccagagctc gttggagacg atgtgtgggc cttcgagcgt
    21001 gccggtgacc tggcggacgc tggtgatgcg ccgttcaccc tcgacccagc cgaggtgcac
    21061 gatgaggtcg acggcgttgg cgacgagcag gttcgtcacc tcgggcacca gccgctcggg
    21121 ggtcatggcc gcgtacatgg cgagccgccc gaagacgccc ttggacgaat cggcgtggat
    21181 cgagcacatc gacccgtcgt tgccttggga catggcgagc agcatcggga gcacctcggc
    21241 gccgcgaacc tcaccgacga tgacgcggtg tgggtccatg cgcagcgcgg agcggacgag
    21301 gtcggcgagg gtgatcgcgc cgacgccttc ggtgttggcc tcgcgggctt cgagggtctc
    21361 gtggtcggga tggaggtcct cgaagcgttc caggccgatc tcgagtgagt cctcgacggt
    21421 gatgagccgc tcgtcggggt cgatctcgtt gatgaggcag cgaagcatcg tggtcttgcc
    21481 ggtaccggtg cccccggcaa cgatgacgtt cgctcgggcc cgcaccgcgg ccgacaagaa
    21541 cccggcgagc aggtcgttgc acacaccgcg tgcgacgagc tggtcgacac ggtggatgtc
    21601 gaagtcgtgg cggcggatcg tcaccgtggg tcgacctgac acggccatga gggcgtggag
    21661 ccggtcgccg ttggggagtt ggaggttgag ctcgggctgg gcgtggtccc agcggcgttc
    21721 gcttcgacca agtcgccggg cggcggtggc gatcagctcg accagctcgt cgtcggacgc
    21781 ggcgaccgac gggccacgca ccttggtgcc gtcgcgcatg gtgagccaca cccggttcgc
    21841 ccccgagatg tggatgtcgc ggatcgacgg gtcatccagc agcggttgga ggcgggcgag
    21901 gccgtggagt cgatcgagca ccgcggtcat caccgcgtcc tcggtggctt cgtcgagcgg
    21961 ttggcgacct gtggagtagg cgtctgttgc cagtcggcgc agctcgtcgg ccaacaactt
    22021 gcgggccagg gcccgttcat cgggtaccga caggcggccc tgaccctcgt cctcatgggc
    22081 gctgtgggcg gcggtgagcg attcgccgac gatgcgatgc aatcggccga cgagctccgc
    22141 ggcctcgccc tcgagcgggg ccgacgacgc ggtcgagctc gtgggtggcg tgagtgcgac
    22201 ggtggcgctc atccgaccgt cacgacgtca gggacttggg cggcgggctg gtcgatgagg
    22261 tccacgagcc cggcaacggc tcgagagagc cgggagcggg cgagggatcg ggaggtggtt
    22321 cccccgtcgc ggaggtgggc ggcggtcgtg gggtcgtcgg gcaggtgggc caggaccggc
    22381 gcgccgagtg agtcaccgat ctcggtgggc cggtaggtgc cgtcaccgac gagcaccacc
    22441 ccaacctggg cgccgcggtg gccgaggttg gtggcgttca gcgttgcgac cgcgtgggat
    22501 gcggcgacga gctcctcggc attgggtcgg gccagcacca acgccacctc ggcggcgtcg
    22561 aggatgcggg ggcggcgcca gatccggccg acgtcgatga cgaccgttcc gttggagcgg
    22621 ccgaggacgc ccatgatccg ctcccccgct gttcgccaca gtgactccgc tgtctccgcg
    22681 gcgtccgggc cgacgagcac cgggatgccg ccggcgttct gggcgtggtc gagccaaccg
    22741 ccgggctcgt gcggattcgc cgcagccaac gtgaccaggc ccggttcgcg tccgaggccg
    22801 taacgcaccg cgaccacacc accggacagg tccgcctcca ccatcacggc gtcgaggtgc
    22861 gacgcgacga gcagagcggt ggtggtgacc ccgggtgctc cccgggccga gcacaacgcc
    22921 acggatctca tggcgacacc tgcacgagcc gcaacgaccc cgaccccgcc cgggccgcga
    22981 ccgcaaccgc gtcgccctcc ggcgcgagga tcgacacgag cagtcggtca ctcgccagct
    23041 cctcgaccga gacgaccgtc gccccgcggg cgatcaccac gtcgccggtg ccaatgctgg
    23101 cgtcagcacc gtcgtcgctt gtgtcgcctt ggtcggtgcc gtccgtgatc gggtcggcgg
    23161 ggtcgacggt tcggaccacg ttgacgaggt cacctggcga cagtcccatc gctgggtagc
    23221 cgcccggttc gagcgacagt ccgacgatgc cgtcgcccgc ctcgagcgca ggacggtctg
    23281 cgacgaccga gtgcgtgagc agggttcccg gtgccaggtc gacgagcgcc acctggccga
    23341 ccacgctgtt catgtcggct tgggtcaggt gggcgagcct gtcgtcgctg gcgacgtaga
    23401 ccacacgaac atccgatgac gacagggtct ggcctcgttc gacggtgccg atgaccgcaa
    23461 gcgctggtga cttgtcgacg gcgctcaggt gccacaggac cgcgccgaga gcgaacacgg
    23521 tcatcaccag cacgccgacc accagctcgg gcatgcgcac ccggcggcgg ggtggggcga
    23581 tgcccgcgac ggggtcgggg cggtggccgt tcgtgctcgg tgctcccgat cggggcgcgc
    23641 ggacggttgg ggtggatgcc atgtcagtcg cctctcgtgc cgatcgcttg gatctcgccc
    23701 acctcgacat cgagggtcga ggtgcgcgtg atcgccggga gggtcccacc gaacggcgca
    23761 ttggaggtcc aggtgatctc gaggcggacc gtggccgaga ggtcgaaggt gccgccgtcg
    23821 gccgaggctg acgaccggcg gtaggtgtgg gtgcagttgc tggcgtcctc gtcgacgccg
    23881 ggcgaccagg cgaccccggg gccacggcac acttcgctgc caccatcacc ggtgccccag
    23941 gtgaccgaca ccggtcgagc ggtcaccgtc gaccacaccc ggcccgcctg cgcggtagcg
    24001 gtgtagggct gccaccacga cgaatcgatc cacaaccacg tcgggatctg cacgtacagc
    24061 cgtccgctct cttctgggct cgtgcggatc gcgggcggcg cgatctgcac ggactgcaac
    24121 gcctgctgag ccagagcggc cgggtcgacc agaccgccct cggggacgat gaagtagccg
    24181 tcgacagcga cggcgccggt accttcgcac cagtactcga gccagcgtcc cgtcgccgag
    24241 tgctgggtct ggagggtgtc gacgtcgtag atgcccatct ggaagtcgtc ctcgatgacg
    24301 acgtgccact cacacggcgg accttcccct cctcctcctc caccgccacc cgagccgcca
    24361 ccaccggggt tcgcggtgcc gtcgcctgcg gtggccgtcg ggtcgccgtc accgtcgacg
    24421 taggcgccac ctcctccacc tggaggagga cggttgccgg caccggccgg tgtcgcggca
    24481 agcaggaccg aggccccggc gaggagcaaa gccgtcaaga cgcgagtgcg catccggcca
    24541 ccccctcgaa ctgctggagg agtcgagcct ccgacacctt ccacaccccg tcgacccgaa
    24601 gcatctcggc ctggacgttg tgggtgctga ccgagtcatc gaccacctca ccggtgtcac
    24661 ggctcacgac caggccgtca tcgaccacgc actcctggac cacggcccgg tcgccgtcga
    24721 tctccaccac cgtgacccgc tggatgttgt gtggttctgc ggctcgctcg aacgcgacac
    24781 ccgagtcgag gttggactgg gtctcggcaa ccacggcatc gagctgacgg ccagtggcga
    24841 agtcgcgcaa cgccggatcg tcggggttcg ggaccccagc gttcgcgttg aagcgcgcgt
    24901 tccagtagcc gacgtagcga tcgatcacct cctgctccgc ggtcgtaccc ggagcagtag
    24961 tgaccggtgt ggcctcggtc gtggtcgtcg tggtgtcccg cgacgtcgag gacgtcgacg
    25021 cggtggtcga tgtcctgtct gcggcggtcg tcgacgacgc cgcggtatcg ctgtcgtcat
    25081 cgctgcaacc ggcgagcgcg agaaccagcg caacacagag ggcgctgatc acggcgcgtc
    25141 ggggtgggtg accgaaccaa ggcatgggtg tgcctcctcg tccgagggga cccgtaccca
    25201 gcgccgacca tcccaccaac acgggacggc ggcggaccgc acccatgagg gatcgacagc
    25261 gagtggcgct gaggtgggag gtccgccccg tatcggtcag gcagacgaga ccgggccgac
    25321 cacgacgtcg accagcaacg acggtacccg atcgtctcgg agttgtctcc tgacctcggg
    25381 tcgatcccgc tcgagcaccc atcagctcgc cagcggcagg acgcgcaggc agcgctcggc
    25441 gcgggtgcgt cgacgccaga tcgcgtccgc cgacatgttc atgtccgcgg ccacgtcgac
    25501 cagcgactca ccgcccaggc gggtacggat gatcgtcgcc agcgcggcgc gggagaccat
    25561 gccctgctcc tgggcgctgc ggagctcgtc gaacaactcc tgctcgcaca cgacctggtc
    25621 cggggtcggg ttgggatcgg cgccgaagcg ccggttgtgc acctcgggtt cgaccgggca
    25681 ctcgatcgtg gccctgttgc ggaggaactc cttgcggacg tcgagcacca cgttcgccgc
    25741 caccggtccc ggccgctgcg gtgggtaggt gcggatccgg ttccacgcga tcgacacgac
    25801 ctcacccacc gagtccggct cgtcgtgcac caccccggca acgacacgga ccaccccgcc
    25861 gagtagcgcc tggagcaacg tgcgggccgc gatctcatca ccgggagcct cggtggcgag
    25921 cacccgcaac gcgctcgccc cccagtcgcg tcgaccccgg gtgtcgacga agtcgttcaa
    25981 gctctgggcc tggccgagct cggggtggcg ggccgtccag cgcatcagcg ctcgacgagc
    26041 cttcggagag cgggccaacg tggcccactc ctcgttcaga gcatgcacga tcgttgtcat
    26101 cgggaggacc tcctggtgct ggttggacac caggaggctc cgcgcggccc atcccgttgg
    26161 gtctcccgtt acctatccgg ttggcgagaa atcctcgaga ttcttcaacc ggatacccag
    26221 cgggat
//
LOCUS       DAOQOD010000022        25292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_656_length_25292_cov_11.929429, whole genome shotgun sequence.
ACCESSION   DAOQOD010000022 DAOQOD010000000
VERSION     DAOQOD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 25292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25292
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_25292_cov_11.929429"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLZ72_03995"
     CDS             <1..772
                     /locus_tag="PLZ72_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA35496.1"
                     /translation="MAARAGHDLTYLALSGVLAHVGRADQAPTPPLNLVADFGGGGML
                     LALGVLAALVERATSGVGQVVDAAMVDGAALLMAPLFGAWSSGFWSADRGTNLLDSGA
                     PFYDCYRCADGGWLAVAAIEAQFYSALLDGLGLADEDLPDQHDRSGWPVLRSRFAAVI
                     AGRDRDDWAARFEGIDACVAPVLDMGEAPLHPHARERRAFVDVDGVPQPVAAPRFSRT
                     PAAEPQAARTGTDPAVALRPWGVSSKDVSALVAAGVVT"
     gene            complement(790..1488)
                     /locus_tag="PLZ72_04000"
     CDS             complement(790..1488)
                     /locus_tag="PLZ72_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35497.1"
                     /translation="MEKIIHLVRERVDRDGGEWRAEVLGSVVPALVDAGATAVSVHLA
                     DDGVGIAGPAPAPGGELPLRAAIAYWVPAHDRAEQALAAAGSVGLRNDAYLVTEALWS
                     DFGQSRGEPRGWADGERSPGLVTVALVHRNPRLDPRTFRELWYRHQSPMSERVQPRLR
                     YVRNTVVHPVSPGAVPVDGIVIESWPSEEIVSDIEAFHLGDVGNLTIMLDSVQDCFDM
                     DRLRSVAMSEYLFA"
     assembly_gap    1549..1648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1649..2847)
                     /gene="argJ"
                     /locus_tag="PLZ72_04005"
     CDS             complement(<1649..2847)
                     /gene="argJ"
                     /locus_tag="PLZ72_04005"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNA35498.1"
                     /translation="MTAPEDRSEEPAADIELPRGFTAHVANVGIKDATDDFVVISTEV
                     PCAAAAVFTRSRFAGPSVELSRRHVADGSLRAVVVVSKNANVATGEAGRADAEELAAG
                     VAGAIGADPSDVLVASTGVIGRRYPMDRVRSHLARLATPFPITSALPVATAMMTTDTH
                     PKVAARTVQTRSDGVGGPARVVGVAKGVGMIEPDMATMLAFVFTDAEVAPDVLDAAFR
                     RVVDRTFNSLSVDTDTSTSDTAAVLASGAAGAVDADTLEQVLGEVCLDLTRQLAADGE
                     GAETLIVVEVRGARDDAQAKRVAKAIVNSPLVKTAVHGADPNWGRVAMAIGKCSDDTD
                     IDQDRVRIRFGDREVYPTDPGPDGLADLETYLKGDEVLIGVDLGVGDERDAGRWTVYG
                     CDLTDGYV"
     gene            2929..3747
                     /locus_tag="PLZ72_04010"
     CDS             2929..3747
                     /locus_tag="PLZ72_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA35499.1"
                     /translation="MNPSEPTRSPTSLEGRRILVTGGAQGLGEAISARIVDLGGQVLI
                     ADVLVDAGRATAERLGDGVEFVELDVSSADGWRDAVTVADDTFGGLDGLVNNAALLHI
                     GLLEDMEPDRIARLLDVNLLGPTLGIRAVAPVLRAAGGGAIVNISSIGGLEGMNSTVV
                     YSATKWGLRGLTRSAAIEYGRDRIRVNAVCPGMGNPEMFTPFLGGFDLERYVDKAPVV
                     PYHEEGRPRNVDMYDVAEMVVWLLSDAARGCSGADYAVDAGWTAGTFAPGLPGF"
     gene            3834..5261
                     /locus_tag="PLZ72_04015"
     CDS             3834..5261
                     /locus_tag="PLZ72_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNA35500.1"
                     /translation="MERHATALQVAAAVASGEVSPSEVLEHYLAEVDRLDPDLNAFAL
                     RDDDRARAEARNADDVVARARRDGIELAPFAGVPIPIKDLYDVAGWVTGHGSLGVTDD
                     PATFDELVVERLRRAGFVLMGKTTTPEFGSVSATECRRTGITRNPWNTDRTPGGSSGG
                     AAAAVASGMAPIGHASDGGGSIRIPASCTGLVGLKPSRGRITSRLYGVVGASTHGVVT
                     RDVADTAALLDVMTELDTGSWNVAPPSGRPYAEEVGAEVGRLRVRLCVENALGFPVDP
                     ECATAARSVADTLADLGHDVVEGAPAWPDAGEFLEKFVTIWNCLAILSPVVDESRLEP
                     HNIAAFEAARATDAFAYLGADAALQEESRAFLSQFPGPRGPGDFDVLVSPTMSIVPKV
                     AGTIMDGVEDMPTAPIMNAVPMACYTSVFNATGQPAISLPAHVSADGLPVGVQVVAAP
                     WREDVLIRLASQVERARPWAALPEL"
     gene            5326..6468
                     /locus_tag="PLZ72_04020"
     CDS             5326..6468
                     /locus_tag="PLZ72_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNA35501.1"
                     /translation="MNATGATITSLTVVPMRLRFHEPFAVTSGVRTHLDTVLVRLTAS
                     DGTVGWGEASPDEGVTGESPTACVEALGGVLAEAVTGRGVDDVDALGSSIDDLADGAG
                     PAARAAVDIAVHDAAARRRGLPLWRFLEDRIVAGGGTRAGRNHLQVSRVVSMDDPEVM
                     AVRAATHVRDGFSTVKLKVGDPHDWRRDVERVVVVRAAVGPDVGIKVDVNQGWRTAEV
                     AVPAVAAMAESRPLFVEQPVDRRDLAGLAEVRRQVPQVPIMADEAVGSAEDLGRIVAL
                     GAADIVNLKVMRLGGLRAAIAADAVAGVAGMRSQIGTMIESSIGSAAGLHLATALANV
                     AFVEMGGPLMLAEDPGDLGRCYRGETVTLPDGPGLGVTPRPPSRFS"
     gene            complement(6506..7108)
                     /locus_tag="PLZ72_04025"
     CDS             complement(6506..7108)
                     /locus_tag="PLZ72_04025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNA35502.1"
                     /translation="MTTNPPPLVLASASPRRAELLSRVGYRFETCAAEVDESVRADEV
                     PTDYVSRLARAKADAVWESGLVIVASDTCVVAPDGTVLGKPADAGDAIGMLRSLSGRT
                     HSVLTAVCVIGPDGPSSEVLGSARVTFAHVDDATLAGYVAGGEPMDKAGAYALQGQGA
                     ALVESIEGDPTTVIGLPLRPTIDALRKLGLPPPAFTSDRP"
     gene            complement(7121..8314)
                     /gene="dusB"
                     /locus_tag="PLZ72_04030"
     CDS             complement(7121..8314)
                     /gene="dusB"
                     /locus_tag="PLZ72_04030"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNA35503.1"
                     /translation="MSIEVRTLPLGQFQIDPPVVLAPMAGVTDAPFRVLCAEFGGGLF
                     VNQMVTARALVENHPTSWSLTRFHPRESVRSLQLYGTDPGYLSEAVRRLVGDGRVDHL
                     DLNFGCPAAKVTRNGGGAALAFKRRLLRRVVEAVVTAADAESGGLVPVTVKFRLGIDD
                     DHLTYLDTARISVEEGAAWVALHARTALQHYAPTARWEAIAELVAAVDSVPVLGNGDV
                     FTAADALAMFERTGCDGIVIGRGCLGRPWLFGQLDDALSGRPVRPEPTLGEVVDTIRR
                     HLALIVEWGELTGDGLHMDGPLRGTVRLAPFRKHLAWYLKGYPVGGDVRRTAGQVGSL
                     DELDRLLDGLTPLADTRPVAEPATIARSHHHALKRVALPDGWLDDPDEDIRLSAAAEA
                     LVSGG"
     gene            8334..9281
                     /locus_tag="PLZ72_04035"
     CDS             8334..9281
                     /locus_tag="PLZ72_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNA35504.1"
                     /translation="MTVTANPPDPNTRIEAGTRIQPGILDDSATSFCAQQYLVTAGVD
                     VDALRRALGEIRDLAGSDRGPSRVVVAFGASLLGTLAPETMPSGMRPFTAIEGLDGHS
                     VPATQDDLFLWFTAGSADRCLSGAWRSRRLLEGLAELTAETPGFQFFDHEDITGFEDG
                     TENPTGEDRLEVAAIPDGPAAGGSFVLAQRWVHDLRAFEALSLRDQEHVFGRTKVGSV
                     ELDPLPERSHVERVVMEDERGDEREIYRRSFPYGTTDEQGLFFLAFNRDLETFQDMLE
                     RMLGAADGQRDKLTDVSAPVTSSFYLAPSVEVLDRIVAG"
     gene            9566..11293
                     /gene="leuA"
                     /locus_tag="PLZ72_04040"
     CDS             9566..11293
                     /gene="leuA"
                     /locus_tag="PLZ72_04040"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNA35505.1"
                     /translation="MSGTSPTKMPFEKYRPFLPLSPEQGGSANGGLLDRSWPNNVLDR
                     APIWCSVDLRDGNQALIDPMDPVRKMKMFTTLVDMGFKEIEVGFPSASQTDFDFLREI
                     IEGDRIPDDVTIQVLVQCRADLIERTYEALRGARSAIVHFYNSTSVLQREVVFGKDRA
                     GITQIATDAARLVRKYEDTLADTDIRYEYSPESFTGTEPDYAIEISAAVLDALDLGAE
                     EKVIINVPATVEMYMPNVYADVVEYVHRNMPHRDQVILSLHPHNDRGTAVAAAEMGLL
                     AGADRVEGTLFGNGERTGNVDLVTIGLNLFTQGVDPELDFSDIDALRRTAEYCNRLPV
                     HERHPYVGDLVYTAFSGSHQDAIKKGTEALGVGPTGGTDDYEAWQVPYLPIDPAHLGR
                     TYDAVIRVNSQSGKGGVAYVMKAEYGLDLPRRLQIEFSKEIQHVAEDTGTEITPSQMW
                     QAFEETYLSDESGDIVFRSAEATTNAERSRVTAQLVVAGEPRTVVGEGGGPISAFVRA
                     LRDDLGIELEVVDYAEHAVSAGTDATAAAYVEAKFPDGTVRWGVARDESILTASLKAV
                     VSAANRSRG"
     gene            11325..12404
                     /locus_tag="PLZ72_04045"
     CDS             11325..12404
                     /locus_tag="PLZ72_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA35506.1"
                     /translation="MTDFDQIDRAEVDATIHAVQAEIGTSFTWDYERSRPGLSRLYSK
                     AKGSQWNATTDLDWSIDVDPERVSADLGGSSPVGRFGHLVDHPDSPVRSFGESEWTTV
                     GVELQNSILSQFLHGEQGALLCTSRIVETVPWIDAKYYASTQVMDEARHVEVFARYLD
                     EKLGRQYAINPNLKALLDDILTDSRWDVTYLGMQIMVEGLALAAFGFMRQATNEPLLQ
                     DLLRYVMSDEARHVAFGVLSLQEYYGGLTSDELRERQEFAFDAAVRLQKRFRNLEVWE
                     RMGVDGQAVSDFLDTHSYEQADVFQTMLFSKIVPNCKKLGLLDAADGWLRDRFTEIGV
                     IQFEDWVDTSEEYEALDEVAADRAG"
     gene            complement(12483..12878)
                     /locus_tag="PLZ72_04050"
     CDS             complement(12483..12878)
                     /locus_tag="PLZ72_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNA35507.1"
                     /translation="MATTAPNAPVTEALNELRLRGVGALVVSTDGVHIEGIVSEREIV
                     HRLAERGEAFIHEPVEAVMITEVVTCQPDDDLQQLARRMTETRCRHLPVVVDGQLVGI
                     VSIGDVVKARLTQLEEESRQMQEYITTGR"
     gene            13111..13602
                     /locus_tag="PLZ72_04055"
     CDS             13111..13602
                     /locus_tag="PLZ72_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35508.1"
                     /translation="MHAHDALGRFTETVDRRAPGLVRFVAGLRWGGLRTVDPDGWTWG
                     AEAAAGVGLGLGARGALRVASGDRVRVPLLPAVLVGWLGSHLAVWRWDTLRWRKRRVA
                     LVVDLPVEGVVAIAEQLGADGRPVQRWEKGSGRSRVHGLWCRAGDVRAVNRAIDAARV
                     RRP"
     gene            complement(13618..15042)
                     /locus_tag="PLZ72_04060"
     CDS             complement(13618..15042)
                     /locus_tag="PLZ72_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNA35509.1"
                     /translation="MSEPTATSGRRRPGRPGRDRRPHLRATAAIAITTVVGVIGACTG
                     TSGQQVEVAAEDRADDRSTTSTTQAPDCAQMLPVEARAAQLMLVMVTSPNAAADALAA
                     GQVGGFGLKGNQTKDVGSQIADAVEGAPITPMVGADEEGGTVQRLRLALGEIPSAATV
                     AEGTPEEAAQQAGEHAAAMKQLGFNVNFAPVADVGSGSGLGTRSYGDDPATVSRFVEA
                     VWPAMQRKGVLPVIKHWPGIGGGSADPHAKLTSLDSIDELRGRDLVPFRTAITAGVPA
                     IMVTHAEVPGLTQDGEPASLSSAAITGELRGTEGFKGLVITDSLGMGAIARTDTQAEA
                     AEKAITAGADIAMVSGSEVVPEVHARLVEAISSGRIPPDQVLASVRRVLAAKGVDGQC
                     LDAVARYSAIARQGTTTTAATGGATQTVLPGSSVPPGPSGSSSTSSTVRNGTTSSGSG
                     RGGSTSTTSTPRRSSTTVVTGIND"
     assembly_gap    15207..15306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15307..16371
                     /locus_tag="PLZ72_04065"
     CDS             <15307..16371
                     /locus_tag="PLZ72_04065"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HNA35510.1"
                     /translation="ATSQLGPRLIRRFMEDPVTQVTLGLFLSTVVFCALTLGSVRTGI
                     GSAGVPAVSYGVSMLLGLSCFVALVIYIHRVATTIQSPNVVASVVRALDRSLDERQAM
                     LEGAEFSLDDGAVHALREQAAREGDVVVAHDSGFVQGIDHLRILDAVDGRDAYVVMEH
                     RVGQFVVEGQPLARVVPASAADRLAGELHHSVEIGPWRTQRQDAEYAIYQVVEIAIRA
                     LSPAVNDTFTGMVCVDWLGAALARMGRDPDGNGGLEGSGGRVRLYIPPLRFERAVRAA
                     FDLIRQAGAANPAISIRLLDVIATTANDVRPEHRPALRAAADQVLAGASARGPIGQDA
                     DDIVERHLRTIATIEALGSG"
     gene            16368..19631
                     /gene="lysS"
                     /locus_tag="PLZ72_04070"
     CDS             16368..19631
                     /gene="lysS"
                     /locus_tag="PLZ72_04070"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNA35511.1"
                     /translation="MIPPIGHERGRSGRFAYPTTARSSISVGELRRTCGSTIDDGTVA
                     GAGGGPVEVVGRTMLVRDHGGIAFVVIQQEGERVQLVVTRVGDEGLLVLPERGDVVAA
                     RGTVGRTRTGELSVFTDRITVLAPHLLPPPDKHQGPREPGRRARERELDLIANPDSRR
                     TFAIRSTVVHALRSELHDRGFTEVETPVFASSAGGAVARPFVTHHHALGIDLYLRIAP
                     ELYLKRLVVGGMDRVFEIGKVFRNEGIDAAHNPEFTILEAYQCPGDQFDMMDLVEQLV
                     VAAGRAAGSTEVDVDGNRIDLARPWRRVAMVDLVEDVTGQRIDPAGDLAAAHAALDEL
                     GVTWEEPWGPGRCLLAAFEERVESTLVEPTIVHDHPLETSPLARAHRSVPGVAERFEV
                     FVAGRELANAYSELNDPVQQRERMESATAIGAEVSEVDEGFLRALERGLAPTGGLGIG
                     VDRLVMLLSGATSIRDVILFPTQRPERRGGEVTAPRPVLPTTPLPTTARPGAPVAVVR
                     LPGLVRVVATLTTVVGVLTMLSALPGLGVRVLPLDELLSPLPQVVDDRVLAVGLGLCM
                     VLLAGQLLRGKRRAWKLAVGLFALSAVISVLRGGEVVALGASLSMLVVLGLTRREFTG
                     LRDPPAITQVLLAAPRFLLFVYGYGFLALWSQRNRLEPPFGWIRSAEAITVGLFGAAG
                     PYDYSGRFATWFPASLVVLGVIGLLLLLWLLLRPAVAAAVGDSDAAGNRARAEDLVRE
                     WGWDTLAPFALRRDRSWFFSSDGRAMIPYGYLGGFALGSGDPIGDPSSIPLAVDEFIE
                     HCRHHGWQPAFLAAREIDAAFYSERGFRDFYLGDEAVLDCRTFDLDAPGMAPVRQSVR
                     RVEGTHRVDLIAESEADPALVARLNEISVRWRKGADERGYTMAMSTEVGASDPGRLLA
                     VAWRLMPGEPGTDATAEVPVAFLRLIPTGGGDGPYGRGYTLDLMRRLPDAANGIIELL
                     VARTVAELDQRGVRRLSLNFAAFSRLLDDDVQHTRVDRLLRRVVDVLNPYYQIRSLRE
                     FNEKFQPEWQPRSLIYADPGDLPKVALRYAFLEGFVDVPLIGRLLSGPSEGGAIGPR"
     gene            19645..20064
                     /locus_tag="PLZ72_04075"
     CDS             19645..20064
                     /locus_tag="PLZ72_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35512.1"
                     /translation="MSDERPGPLDRMRDPDADPARDRARLRAAAMMAGMGIAHFLVPG
                     PFRRIVPRWFPWRREAVMVSGVAEIASGALLAIPRTRRAGGWLMLLTVLAVYPANVQM
                     AIDASRGRSQVRVPVWLTWLRLPLQLPMLARAWSFTR"
     gene            20046..21314
                     /locus_tag="PLZ72_04080"
     CDS             20046..21314
                     /locus_tag="PLZ72_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA35513.1"
                     /translation="MELHPLSGHGHDPSSRPVASLPGFADRSVLESRVDAASGAGRPF
                     GAERPVGLVRVSGPGDRAAELADRLRSAVRPGDTVAELGAGCFAVLCDRLGEPDDLLT
                     IAHRVEQQLTGVPPLDPRLDVALVDHAGREPARIVGDDRPGRRAEDPEDLPSGTWVRF
                     DEALRDRATRREQTEAALRRSSDGRDLVLHYQPERDLVTGRTAGVEALLRWERDGVTV
                     GPDRFVPLAEHTGLIVPIGEWVLRTACDQLLRWRRDGAARDAFVAVNVSPRQLMHDGF
                     VVSVREALADPGLAPGDLVLELTEGVLLNDLALARDRLGELRGLGARVAVDDFGTGYA
                     SLTYLHQLPIDAVKLDRSFVSGLGVDPRLTAIVTSVLGLLGALGLQSIAEGVSSAAQA
                     ERLVALGCALAQGEWVGTPAPPEQLVDQLR"
     gene            complement(21330..22823)
                     /locus_tag="PLZ72_04085"
     CDS             complement(21330..22823)
                     /locus_tag="PLZ72_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA35514.1"
                     /translation="MEETDGETATPLDDRLLDDRLLDDRLLDDLEDALGAGSVLRAGR
                     DVLDRYLEPARGRGGPAAAVLRPATTAQVCTVVRHAVLDGFRLLPQGANTGLVGASVP
                     PTVPSGSDPRPIVVLSTERLVDPIEVFADDRVAIVPAGVRLSTLNDRLAPLGLSLPID
                     LGADPCLGAMVATDTGGARMLAHGDMRRRVLGVEAVLADVELTNGSGPTVIDDLTRLR
                     KDNTGPRLTSLMVGSGGAFGVVTRVAVELARVPRESACGLLAPVDAAAALDALRILED
                     ALGPLLSAYEVVSDTALDLAVDHIDGLRSPWGDRRPAPEVTVLVEAAGPIGTAEVLAD
                     AVAALDDAGVVDDAVLVPTDRAWHLRHSITEGLARAGTVVGFDVSIPRSELVAFRDDV
                     RERLATTVPRAMVADFGHWADGGTHCNLVFPDGPPGDSEREDARRVVFGTAVDDHHGS
                     YSAEHGIGPHNADWWIRTTPPGDRAVLRSLRDRCDPHRVLGHPALPF"
     gene            22872..25214
                     /locus_tag="PLZ72_04090"
     CDS             22872..25214
                     /locus_tag="PLZ72_04090"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNA35515.1"
                     /translation="MTTTDAEDPTAPYAGHEGRVGRVFATSEPWWPERRRPAEGAPNI
                     VVVLCDDLGYSDLGCYGSEIPTPNIDAIAAEGLRYTDYHSAPMCSPTRASLMTGLEPH
                     RAGVGHVAHSDPGFPGYAMELTPFAPTLPELLRDAGYQTLMVGKWHLTKDSEVNDAGD
                     RSSWPLQKGFDRYYGFLDAFTNFHHPHRLIEDNHAVRTDEYPEGYFVTDDFTDRAIDM
                     VRESKASDPTKPFFLYFSHAAVHAPLQAKAVDVERHAETYRAGWDAIRQARYDRQVDL
                     GVIPDGAELAPRNTESGDDVPPWDELDDDQKRLFARYMAVYAGLIDNLDQNIGRLRGA
                     LEEMGEWDNTLFIFTSDNGGSREGQEQGTSAYFRTLHWERKGAAEPFEEDLARHDVMG
                     GPTTLPHYPRGWAMVSNTPFRLYKINAHRGGHSVPFVVSWPRALADSGLAGGGLREQY
                     LHVTDLLPSFLELAGVTRPDEWRGNPVLPLAGEAVFGPTITDPDAVGRSRDVVVENEG
                     HRGYRRDGWEVVTRHPARTPFSADHWELFDMAADPTQLRDLSDEHPDRRDELVEAWES
                     AAWENQIFPMDEGTGYRFLLRPPWVERYDEPVVLHAGMPTLDRWRSQRLILWRDAAII
                     ADVTLGEGDRGILFAHGDQGGGYSLYVDDTGELVAAHNGYGLEGEVRGPVVGPGRHEL
                     ELRITCPGDDLWDLAVAVDGEAVAAGTGFRLLMAMAPFEGIDVGLDRRSPVCWKVYER
                     HGSFPFTGTIHSVRYQPGDPAPDSPTNFLEVLRDWGRSME"
BASE COUNT         3421 a         9000 c         9015 g         3656 t
ORIGIN      
        1 catggccgcc cgcgccggac acgacctcac ctacctggcg ctgtccggag tgctcgccca
       61 cgtcggacgg gccgaccagg cgcccacccc accgctcaac ctggtggccg acttcggcgg
      121 cggcgggatg ctgttggcgc tgggggtgct ggccgcgctg gtcgagcggg caacctccgg
      181 cgtcggccag gtggtcgacg cggccatggt cgacggtgcc gccctgctga tggcgccgct
      241 gttcggggcc tggtcgtcgg gtttctggtc ggcagaccgt ggcaccaacc tgctcgactc
      301 cggcgcaccg ttctacgact gctaccggtg cgcggacggc ggctggctcg ccgtcgcggc
      361 catcgaggcg cagttctact ccgctctgct cgacggtctg ggactggccg acgaggacct
      421 acccgaccag cacgaccgca gcggttggcc tgtcctccgg tcgcggttcg ctgccgtcat
      481 cgccgggcgc gaccgcgacg actgggccgc ccgcttcgag gggatcgacg cctgcgtcgc
      541 cccggtgctc gacatgggcg aggcgccgtt gcacccccac gccagggagc gacgggcgtt
      601 cgtcgatgtc gacggggtcc cccaaccggt cgcggccccg cgcttctcgc gcactccggc
      661 ggcggagccg caggcagcgc gcaccggcac ggaccctgcc gtcgcgctcc ggccctgggg
      721 tgtgtcctcg aaggacgtca gcgcccttgt cgccgccggc gtcgtcacct gagcggcaac
      781 cggggcaggt caggcgaaga ggtactccga catggcgacg ctgcgcagac ggtccatgtc
      841 gaagcagtcc tggacgctgt cgagcatgat ggtgaggttg cccacgtcgc cgaggtggaa
      901 cgcctcgatg tcggagacga tctcctcgga gggccacgac tcgatgacga tcccgtcgac
      961 cggcaccgcg ccggggctga ccgggtgcac gacggtgttg cgcacgtagc ggagtcgcgg
     1021 ctgcacccgc tccgacatcg gcgactggtg ccggtaccac agctcgcgga acgtgcgggg
     1081 atccaggcgg gggttgcggt gcaccagcgc gacggtgacc agcccgggag agcgttcacc
     1141 gtcggcccag ccgcgaggct ctccccggga ctggccgaag tccgaccaca gcgcctcggt
     1201 gaccaggtag gcgtcgttgc gcagaccgac cgatcccgcc gccgcgaggg cctgctcggc
     1261 gcggtcgtgc gccggcaccc agtaggcgat cgcggcgcgc agcgggagct cacctccggg
     1321 ggcgggcgcc ggaccggcga tcccgacccc gtcgtcggcc aggtgcacgc tcaccgcggt
     1381 cgctccggca tcgaccaacg ccgggaccac cgatccgagg acctcggcgc gccactcgcc
     1441 gccgtcgcgg tcgacgcgct cacgcaccaa gtggatgatc ttctccatgt gacccccgat
     1501 tcgtgttctc tgggttcgtg atcacccgtc ccggatcgct ccgggatcnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gtagccgtcg gtcaggtcgc atccgtacac
     1681 ggtccatcgt ccggcgtcgc gctcgtcgcc gacgccgagg tcgaccccga tgagcacctc
     1741 gtcgcccttc aggtaggtct cgagatcggc gagcccgtcc gggccgggat cggtcgggta
     1801 gacctcgcgg tcgccgaagc ggatccgcac ccggtcctgg tcgatgtcgg tgtcgtcgga
     1861 gcacttcccg atcgccatcg ccacccgacc ccaattgggg tccgcgccgt gcaccgcggt
     1921 cttgaccagc ggcgagttga cgatcgcctt ggcgacccgc ttcgcctggg cgtcgtcgcg
     1981 ggcacctcgg acctcgacca cgatcagggt ctcggccccc tcgccgtccg ccgccaactg
     2041 gcgggtgagg tccaggcaca cctcgcccag cacctgctcc agggtgtcgg cgtcgaccgc
     2101 tcccgccgct ccgctggcca ggaccgcagc ggtgtcggag gtggacgtgt cggtgtcgac
     2161 gctcaggctg ttgaaggtcc gatcgaccac ccgtcggaac gcggcgtcga gcacgtcggg
     2221 ggccacctcg gcgtcggtga acacgaacgc cagcatcgtc gccatgtcgg gctcgatcat
     2281 gccgacaccc ttggcgacgc cgaccacccg ggccgggccg cccaccccgt cggaacgggt
     2341 ctggaccgtc cgggccgcca ccttggggtg ggtgtcggtg gtcatcatgg cagtggccac
     2401 gggcagggcc gaggtgatcg ggaacggcgt ggccagccgc gccagatgcg atcggacccg
     2461 gtccatcggg tagcggcgac cgatcacccc ggtcgacgcc accaggacat cggacggatc
     2521 cgcgccgatc gccccggcca caccggcagc gagctcctcg gcgtcggcac gaccggcttc
     2581 gccggtggcc acgttggcgt tcttggacac gacgaccacc gcgcgcagcg acccatccgc
     2641 gacgtgacgc cgcgacagct cgacgctcgg tcccgcgaag cgggaacggg tgaacacggc
     2701 ggccgccgca cagggcacct ccgtgctgat gaccacgaag tcgtcggtgg cgtccttgat
     2761 cccgacgttc gcgacgtgag cggtgaaccc ccgggggagc tcgatgtccg cagccggctc
     2821 ctcggatcgg tcctctgggg cggtcatggc ccagatcctt gcagagccca cggggtgaca
     2881 gactcccgat accggcgcgt cgccggtcgc cgacaacagg gggcagccat gaacccgtcc
     2941 gagccgaccc gctcgcccac ctcgctcgag ggccgccgga tcctggtgac aggcggtgcc
     3001 caggggctgg gcgaggcgat ctcggcacgg atcgtcgacc tcggcggcca ggtcctgatc
     3061 gcggacgtgc tggtcgacgc gggccgggcc accgcggaac gactgggcga cggggtggag
     3121 ttcgtcgagc tcgatgtcag ctccgccgac gggtggcgtg acgccgtgac cgtcgcggac
     3181 gacacgttcg gtgggctcga cggcctggtc aacaacgcgg cgctgctgca catcggcctg
     3241 ctcgaggaca tggagcccga tcggatcgcc cggctgctcg acgtgaacct gctcggaccc
     3301 accctgggga tccgggcggt ggcgccggtg ctgcgcgccg cgggtggggg agcgatcgtg
     3361 aacatctcgt cgatcggtgg gctcgagggg atgaactcga cggtggtcta ctcggccacc
     3421 aagtggggac tgcgcgggct cacccgctca gcagccatcg agtacggccg ggaccgcatc
     3481 cgggtcaacg cggtgtgtcc cggcatgggc aaccctgaga tgttcacgcc gttcctcggc
     3541 ggcttcgacc tggagcgcta cgtcgacaag gcgccggtcg tgccctacca cgaggagggg
     3601 cggccccgga acgtcgacat gtacgacgtg gccgagatgg tcgtgtggct cctgtcggac
     3661 gcggcccggg ggtgcagcgg tgccgactac gccgtcgacg ccggttggac cgccggcacc
     3721 ttcgcccccg gcctgccggg gttctgagcg gaggctcggg ctggtccgcc gaccggtgag
     3781 ccaccccgct cccacccctc ccggaggcgc gggcccgggg tagcgtcgcc gcgatggaac
     3841 gccacgccac cgccctgcag gtcgccgccg ccgtcgcctc cggcgaggtc agcccgtcgg
     3901 aggtgctcga gcactacctg gccgaggtcg accgcctcga tccggacctc aacgccttcg
     3961 ccctccgcga cgacgaccgg gcccgggccg aggcccgcaa cgcggatgac gtggtggccc
     4021 gggcccgccg cgacgggatc gagctcgcac cgttcgccgg ggtccccatc ccgatcaagg
     4081 acctctacga cgtggcggga tgggtgaccg gccacgggtc gctcggggtg accgacgacc
     4141 cggcgacgtt cgacgagctg gtcgtcgaac ggctccggcg ggccggcttc gtgctgatgg
     4201 gcaagaccac caccccggag ttcggttcgg tgtcggccac cgagtgccgc cggaccggga
     4261 tcacgcgcaa cccgtggaac accgaccgca ccccgggcgg cagttcgggc ggggcggccg
     4321 cagcggtggc ctcgggcatg gcgccgatcg gccacgcatc ggacgggggc ggatcgatcc
     4381 gcatcccggc gtcgtgcacc ggcctggtcg gcctcaagcc gtcccgcggt cgcatcacca
     4441 gccgcctgta cggcgtggtc ggagcgtcga cacacggtgt ggtcacccgc gacgtcgccg
     4501 acaccgcggc gctgctcgac gtgatgaccg agctcgacac cgggtcctgg aacgtcgccc
     4561 cgccgtcggg ccggccgtac gcggaggagg tcggcgccga ggtcggtcga ctgcgggtgc
     4621 ggctgtgcgt ggagaacgcg ctcgggttcc ctgtcgatcc cgagtgcgcc accgccgctc
     4681 ggtcggtcgc cgacaccctc gccgacctgg gccacgacgt ggtcgagggc gctccggcct
     4741 ggcctgatgc gggggagttc ctcgagaagt tcgtgaccat ctggaactgc ctggcgatcc
     4801 tgtcgccggt cgtcgacgag tctcggctcg aaccgcacaa catcgcggcg ttcgaggcgg
     4861 cccgggcgac cgacgccttc gcctacctcg gcgctgacgc cgcgctgcag gaggagtcgc
     4921 gagcgttcct ctcgcagttc cccggaccgc gcggacccgg cgacttcgac gtcctggtca
     4981 gcccgaccat gtcgatcgtg cccaaggtgg cgggcacgat catggacggc gtcgaggaca
     5041 tgccgaccgc gccgatcatg aacgcggttc cgatggcctg ctacacgtcg gtgttcaacg
     5101 ccaccggcca accggccatc tcgctgccgg cccacgtctc ggccgacggg ctgcccgtcg
     5161 gggtgcaggt cgtcgccgcg ccctggcgcg aggacgtgct gatccgcctg gcgtcgcagg
     5221 tggagcgggc ccggccgtgg gcggcgctgc ccgagctgtg agccgacgtc ggccgctgac
     5281 gcttcttcgc cccgaggtgc tcgcagagcc gggcgcggag tcggcgtgaa cgccaccggc
     5341 gccacgatca cctcgctcac cgtcgtgccg atgcgactgc ggttccacga gccgttcgcc
     5401 gtcacctcgg gcgtccgaac ccacctcgac acggtgctgg ttcgactgac cgcgtcggac
     5461 ggcacggtcg gatgggggga ggcgtcaccc gatgaaggcg tgaccggcga gtcgcccacg
     5521 gcctgcgtcg aagccctcgg cggcgtcctc gccgaggccg tgacgggtcg tggggtcgac
     5581 gacgtcgacg cgctcggatc ctcgatcgac gacctggccg acggggcggg cccagcggcg
     5641 cgggccgcgg tcgacatcgc cgttcacgac gccgcggcgc gtcggcgcgg ccttccgctg
     5701 tggcgcttcc tcgaggaccg catcgtggcc ggcggcggga cccgcgccgg tcggaaccat
     5761 ctgcaggtgt cccgggtggt gagcatggac gacccggagg tgatggccgt ccgcgcagcg
     5821 acccacgtcc gtgatgggtt ctccaccgtg aagctcaagg tcggggatcc ccacgactgg
     5881 cgccgtgacg tcgaacgtgt cgtggtcgtc cgcgccgcgg tcgggcccga cgtcgggatc
     5941 aaggtcgacg tgaaccaggg gtggcggacc gccgaggtgg ccgtgccggc ggtggcggcc
     6001 atggccgagt cccgtccgct gttcgtcgag cagcccgtcg atcgtcgcga cctggccggc
     6061 ctggccgagg tccgccgtca ggtgccgcag gttccgatca tggccgatga ggcggtcggc
     6121 tccgccgagg acctcgggcg tatcgtcgcc ctcggcgcag cggacatcgt caacctcaag
     6181 gtgatgcgcc tgggcgggct gcgggccgcg atcgccgccg atgcggtcgc gggcgtcgcc
     6241 gggatgcgat cccagatcgg cacgatgatc gagagctcga tcggatccgc ggcgggcctg
     6301 cacctggcga cggcgctcgc caacgtcgcc ttcgtcgaga tgggtggtcc gctgatgctc
     6361 gccgaggacc cgggggacct cggccgctgc taccggggcg agaccgtcac ccttcccgac
     6421 ggcccgggcc tgggcgtcac ccctcggcca ccctcccgat tctcatgacc gttcgcgcca
     6481 ccagtggcgc gaacggtcat gagaatcacg ggcggtccga ggtgaacgcg ggcgggggga
     6541 ggccgagctt ccgtagggcg tcgatggtcg ggcgcagcgg caggccgatc accgtggtcg
     6601 gatccccctc gatcgactcg accagagccg cgccctggcc ctgcagcgcg tacgccccgg
     6661 ccttgtccat cggctccccg ccggcgacgt agccggccaa cgtggcgtcg tcgacgtggg
     6721 cgaaggtgac ccgggccgaa ccgagcacct cgctcgaggg cccgtccggt ccgatcacgc
     6781 acaccgcggt cagcaccgag tgcgtccggc ccgacagcga tcgcagcatc ccgatggcgt
     6841 cgccggcgtc ggcgggcttg ccgagcaccg tcccgtcggg ggcgacgacg caggtgtcgg
     6901 acgcgacgat caccaggccc gactcccaca cggcgtcggc cttggcccgc gccagccgag
     6961 agacgtagtc ggtgggcacc tcgtcggcac ggaccgactc gtcgacctcc gcggcgcagg
     7021 tctcgaaccg gtacccgacc cgggagagga gctcggcccg gcgtggtgac gcggacgcga
     7081 gcacgagcgg gggagggttc gtggtcacga tccgttgacc tcagccaccg gacaccaggg
     7141 cctcggccgc agccgacagc cggatgtcct cgtcgggatc gtcgagccac ccgtcgggca
     7201 gcgccacccg cttcagcgcg tggtggtggc tgcgggcgat cgtggccggt tcggccaccg
     7261 gacgtgtgtc ggcgagcggg gtgagcccgt cgagcaggcg gtcgagctcg tcgagcgagc
     7321 cgacctggcc ggccgtccgc cggacgtcgc ccccgacggg gtagcccttg agataccagg
     7381 cgaggtgctt gcggaacggc gccagtcgga ccgtcccgcg gagtggtccg tccatgtgga
     7441 gcccgtcgcc ggtgagctcg ccccactcga cgatcagagc gaggtgacgc cggatcgtgt
     7501 cgacgacctc gcccagggtg ggttccggcc ggaccggtcg accggagagg gcgtcgtcga
     7561 gctggccgaa cagccacggt cggccgaggc acccgcgacc gatgacgatg ccgtcgcagc
     7621 cggtgcgctc gaacatggcc agggcgtcgg ccgcggtgaa cacgtcgccg ttgccgagga
     7681 cgggaaccga gtccaccgcg gcgaccagtt cggcgatggc ctcccaccgg gcggtgggcg
     7741 cgtagtgctg caacgcggtg cgggcgtgca gcgccaccca ggccgccccc tcctcgaccg
     7801 agatccgcgc cgtgtcgagg tacgtcaggt ggtcgtcgtc gatgcccaac cggaacttga
     7861 ccgtgaccgg gaccagaccg cccgactcgg catcggcagc ggtgaccacc gcctcgacca
     7921 cccgacggag caaccgccgc ttgaacgcca gcgccgcgcc tccgccgttg cgggtgacct
     7981 tggccgccgg gcaaccgaag ttgaggtcga ggtggtcgac ccgaccgtcg ccgaccaggc
     8041 gacgcaccgc ctcggacagg tacccggggt cggttccgta gagctgcagc gaccgaacgg
     8101 actcgcgggg gtggaaccgg gtgagagacc acgatgtcgg gtggttctcg accagggcgc
     8161 gggcggtcac catctggttg acgaacagcc cgccgccgaa ctcggcgcac agaacccgga
     8221 acggtgcgtc ggtcaccccg gccatcgggg cgagcaccac cggtgggtcg atctggaact
     8281 ggcccagggg gagtgttcga acctcgatgg acacgctcct agtgtggtcg gcgatgaccg
     8341 tgaccgcgaa cccgcccgat ccgaacaccc gcatcgaggc gggcacccgc atccagccgg
     8401 gaatcctcga cgattccgcg accagcttct gcgcccagca gtacctggtc accgccggcg
     8461 tcgacgtgga cgccctgcga cgggcgctgg gtgagatccg cgacctggcc ggtagcgaca
     8521 gggggccgtc gcgggtggtg gtcgcgttcg gcgcgtcgct gctcggcact ctggcccccg
     8581 agaccatgcc gtcggggatg aggccgttca ccgcgatcga ggggctcgac ggacactccg
     8641 tccccgccac ccaggacgac ctgttcctgt ggttcaccgc cggatctgcc gaccggtgcc
     8701 tgtcgggtgc atggcggtcc cgccgactgc tggaggggct ggccgagctg accgctgaga
     8761 cccccggctt ccagttcttc gaccacgagg acatcaccgg cttcgaggac ggcaccgaga
     8821 acccgaccgg cgaggaccga ctcgaggtcg ccgccattcc cgacggcccc gccgccggcg
     8881 gctcgttcgt cctggcccag cgctgggtgc acgacctgcg ggccttcgag gctctgtcgt
     8941 tgcgcgacca ggagcacgtc ttcggtcgga ccaaggtcgg ctccgtcgag ctcgacccgc
     9001 tcccggagcg ctcgcacgtc gagcgggtgg tcatggagga cgagcggggc gacgaacgcg
     9061 agatctaccg gcgttcgttc ccctacggca ccaccgacga gcagggcctg ttcttcctgg
     9121 ccttcaaccg cgacctcgag acgttccagg acatgctgga gcgcatgctc ggtgccgccg
     9181 acgggcagcg cgacaagctc accgacgtgt ccgcgccggt cacctcgtcg ttctacctgg
     9241 ccccgtcggt cgaggttctc gaccgcatcg tcgccggctg aggccgacgc ggccgtcgac
     9301 cgggcggcgc acccgcagtc ggtcggtgcg ttgccgtcgg ccgggttcgg ggcgtagggt
     9361 ggtcagcgat gtcggccgct tcctccctcc tcctgctcat gtaggcgagt cgcccatacc
     9421 gcgactcgcc gaacctcctg cgctcccgca ggaggtttct tggttttccg gcccggtcca
     9481 cgtgcccggg gcgacgcagg tggggtcgat ccgctcgact cgacccgaca gcacacatcc
     9541 gtcagctcag aacaggacca ccgccatgtc aggtacctca cccacgaaga tgcccttcga
     9601 gaagtaccgg ccgttcctgc ccctctcccc ggagcagggc ggatcggcca acggcggcct
     9661 cctcgatcgc agctggccga acaacgtgct cgaccgggcc ccgatctggt gctcggtcga
     9721 cctgcgagac ggcaaccagg cgctgatcga cccgatggac ccggtccgca agatgaagat
     9781 gttcaccacg ttggtggaca tgggcttcaa ggagatcgag gtcgggttcc cgtccgcgtc
     9841 gcagaccgac ttcgacttcc tgcgcgagat catcgagggc gaccggatcc ccgacgacgt
     9901 gaccatccag gtgctggtgc agtgccgcgc ggacctgatc gaacggacct acgaggccct
     9961 gcggggggcg cggtcggcga tcgtccactt ctacaactcg acctcggtgc tgcagcgcga
    10021 ggtcgtgttc ggcaaagacc gcgccggcat cacccagatc gccaccgacg cagcccggct
    10081 ggtgcgcaag tacgaggaca cgctggccga caccgacatc cgctacgagt actcgccgga
    10141 gtcgttcacc gggaccgagc ccgactacgc catcgagatc tccgccgcgg tgctcgacgc
    10201 cctcgacctc ggcgccgagg agaaggtcat catcaacgtg ccggccacgg tcgagatgta
    10261 catgcccaac gtgtacgcgg acgtcgtcga gtacgtgcac cgcaacatgc cgcaccgcga
    10321 ccaggtgatc ctgtcgctgc acccgcacaa cgaccggggc accgccgtcg ccgcggccga
    10381 gatgggtctg ctggccggag cggaccgggt cgagggcacc ctgttcggca acggggagcg
    10441 caccggcaac gtcgacctgg tcacgatcgg gctgaacctg ttcacccagg gcgtggaccc
    10501 cgagctcgac ttctccgaca tcgacgccct gcgccgcacc gccgagtact gcaaccgact
    10561 gccggtgcac gaacgccacc cctacgtcgg cgacctcgtc tacacggcgt tctccggctc
    10621 gcaccaggac gcgatcaaga aggggaccga ggcgctcggt gtcggcccga ccggcggcac
    10681 cgacgactac gaggcctggc aggtgcccta cctgccgatc gaccccgccc acctcggtcg
    10741 cacctacgac gcggtgatcc gggtcaacag ccagtccggc aagggcggcg tcgcctacgt
    10801 catgaaggcc gagtacggcc tggacctgcc ccggcgtctg cagatcgagt tctccaagga
    10861 gatccagcac gtcgccgagg acaccggcac cgagatcacc ccgtcgcaga tgtggcaggc
    10921 cttcgaggag acctacctgt ccgacgagag cggggacatc gtgttccgct ccgccgaggc
    10981 gaccaccaac gccgagcgct cccgggtcac cgcccagctc gtggtcgcgg gcgagccccg
    11041 aaccgtcgtg ggcgagggtg gcgggccgat ctcggcgttc gtgcgcgccc tgcgcgacga
    11101 cctcggcatc gagctggagg tggtcgacta cgccgaacac gccgtgtcgg ccggcaccga
    11161 cgccaccgcg gcggcctacg tcgaggccaa gttccccgac gggacggtgc gctggggagt
    11221 cgcccgcgac gagtcgatcc tcaccgcgtc gctgaaggcc gtcgtctcgg cggccaaccg
    11281 ctctcgcggg taacttcggg agtcgaacga tccagggggc tctcatgacg gacttcgacc
    11341 agatcgaccg cgccgaggtc gacgccacga tccacgccgt ccaggcggag atcggcacca
    11401 gcttcacgtg ggactacgag cgcagccgcc cgggcctgtc gcgcctgtac tccaaggcca
    11461 aggggtcgca gtggaacgcg accaccgacc tcgactggtc gatcgacgtc gatcccgagc
    11521 gggtgtcggc cgacctcggc gggagctcgc cggtcgggcg cttcggtcac ctcgtcgacc
    11581 atccggactc gccggtgcgc tccttcggcg agagcgagtg gaccacggtc ggcgtggagc
    11641 tgcagaactc gatcctcagc cagttcctgc acggcgagca gggagcgctg ctgtgcacct
    11701 cgcgcatcgt cgagaccgtc ccgtggatcg acgccaagta ctacgcgtcc acccaggtga
    11761 tggacgaggc ccgtcacgtc gaggtgttcg cccgctacct cgacgagaag ctcggccgcc
    11821 agtacgcgat caaccccaac ctcaaggcgc tgctcgacga catcctcacc gactcccgct
    11881 gggacgtgac ctacctgggg atgcagatca tggtcgaggg cctggccctg gccgcgttcg
    11941 ggttcatgcg ccaggcgacc aacgaaccgc tcctgcagga cctgctccgc tacgtgatga
    12001 gcgacgaggc ccgccacgtg gccttcggtg tcctgtcgtt gcaggagtac tacggcggtc
    12061 tcacctccga cgagctgcgt gaacgacagg agttcgcctt cgacgccgcg gtgcgcctcc
    12121 agaagcgatt ccggaacctc gaggtgtggg agcggatggg ggtcgacggc caggcggtgt
    12181 cggacttcct cgacacccac agctacgagc aggccgacgt cttccagacg atgctgttct
    12241 ccaagatcgt ccccaactgc aagaagctcg ggcttctcga cgcggcggac gggtggctgc
    12301 gcgaccgctt caccgagatc ggcgtcatcc agttcgagga ctgggtcgac acctccgagg
    12361 agtacgaggc gctcgacgag gtcgcggcgg accgggccgg ctgagcgggc ccgtggtcac
    12421 cgatttccgg ggtcgggaaa gcgacaaggt gtgtcgctga gcacccgaat tcctggggtg
    12481 ggtcagcggc cggtggtgat gtactcctgc atctggcgcg actcctcctc gagctgggtg
    12541 agccgcgcct tgaccacgtc gccgatgctg acgatgccga ccagctgacc gtcgaccacc
    12601 accggcaggt gtcggcagcg ggtctcggtc atgcggcggg ccagctgctg caggtcgtcg
    12661 tcgggctggc aggtgaccac ctcggtgatc atcaccgcct cgaccggttc gtggatgaag
    12721 gcctcgccgc gctccgcgag acggtggacg atctcgcgtt cggagacgat cccttcgatg
    12781 tggacgccgt cggtcgacac gaccagagcg cccactcccc gcagtcggag ctcgttcagg
    12841 gcctcggtga caggtgcgtt cggggccgtc gtggccacgg tcgaaccctt catcgacagg
    12901 atcgaggaca ctcgcatgat ctgacctccg gtttctccgg gcccttcccc gcgccgtggc
    12961 cggctgtgtg ctcagtatgc accccggggc cccgtccgtc actgaagtcc tggtcgcggg
    13021 gtgaccgtcg gattccccac accgcccggg tccgggcggt cgacggcccg gcgcggtcgg
    13081 aaccgacgtc gggatcgcca caatggggtg gtgcacgcac acgacgcctt gggccgattc
    13141 accgagaccg tcgaccgtcg cgcccccgga ctcgtccgct tcgtcgccgg gctccgctgg
    13201 ggtggactgc gcaccgtcga tcccgacggg tggacctggg gcgccgaagc cgccgccgga
    13261 gtgggcctcg gtctcggcgc ccggggcgct ctgcgcgtcg ccagcggcga ccgggtcagg
    13321 gttccgctgc tgccggcggt cctggtcggt tggctcgggt cgcacctggc ggtgtggcgg
    13381 tgggacacgc tgaggtggcg gaagcgtcgg gtggcgctcg tggtcgacct gccggtcgag
    13441 ggggtcgtgg cgatcgccga gcagctcggc gccgacgggc gaccggtgca gcgctgggag
    13501 aagggcagcg gtcgctcccg cgtccacggc ctgtggtgcc gggcgggcga cgtccgcgcc
    13561 gtcaaccggg cgatcgacgc ggcgcgggtt cgccgaccgt gacgggatcg gatccgatca
    13621 gtcgttgatg ccggtcacga ccgtcgtcga ggatcggcgg ggcgtggacg tggtgctggt
    13681 cgatccgcca cgccccgagc cgctcgaggt cgtcccgttg cggaccgtgc tcgacgtcga
    13741 cgacgagccg ctcggtccgg gtggcaccga gcttccggga agcacggtct gggtggcccc
    13801 gccggtggca gcggtggtcg tcgtcccctg acgggcgatc gcggagtagc gggcgaccgc
    13861 gtcgaggcac tgcccatcga cacccttggc ggccaggacc cgacgcaccg acgccaggac
    13921 ctggtcgggg gggatgcgac ccgacgagat cgcctcgacc agccgggcgt ggacctcggg
    13981 gaccacctcg ctgcccgaga ccatggcgat gtcggccccg gcggtgatcg ccttctcggc
    14041 ggcctcggcc tgcgtgtcgg tcctggcgat cgctcccatg cccagcgagt cggtgatgac
    14101 cagacccttg aacccctcgg tgccgcgcag ctcgccggtg atcgcggccg aggagaggct
    14161 ggcgggctcg ccgtcctggg tgaggccggg gacctcggcg tgggtgacca tgatcgccgg
    14221 cacgcccgcg gtgatcgcgg tccggaacgg gaccaggtcc cgtccccgga gctcgtcgat
    14281 gctgtcgagg gaggtgagct tggcgtgcgg gtcggcggat ccgccgccga tcccgggcca
    14341 gtgcttgatc accggcagga cgcccttgcg ctgcatggcg ggccacaccg cctcgacgaa
    14401 ccgcgacacc gtcgcgggat cgtcgccgta ggagcgggtg ccgagccccg atcccgatcc
    14461 gacatctgcg accggggcga agttcacgtt gaacccgagc tgcttcatgg ccgccgcgtg
    14521 ctcaccggcc tgctgggccg cctcctcggg tgtcccctcg gccaccgtgg cggcggaggg
    14581 gatctcgccc agcgccagcc ggagccgctg caccgttccg ccctcttcgt cggccccgac
    14641 catcggtgtg atcggcgcgc cctccacagc gtcggcgatc tgggacccca cgtccttggt
    14701 ctggttgccc ttgagtccga acccgcccac ctggcccgcg gccagcgcgt cggccgcggc
    14761 gttgggcgag gtgaccatca ccagcatgag ctgcgcggcg cgggcctcga ccggcagcat
    14821 ctgggcgcag tcgggggcct gggtcgtcga tgtggtcgag cggtcatcgg cgcggtcctc
    14881 tgccgccacc tcgacctgtt ggcccgatgt ccccgtgcac gccccgatga cgccgacgac
    14941 cgtggtgatc gcgatcgccg cggtggcacg caggtgtggc cgccggtcgc ggccgggtcg
    15001 gccgggccgg cggcggccgg acgtggcggt cggctcggac atgggcgacc accgtagcgc
    15061 cgcggcggtc gggggcttct gcgccgccgg tccagtcggt cacgggacgc cgcggcggcg
    15121 tcggacagac tgtcgtcgat gccgagcacc ctcacccgtc gacgatcccg ggcctcggag
    15181 tggtggtggg agctgaccta ccgggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnngcca cctcccagct cggcccccgg ctgatccgcc ggttcatgga ggaccccgtc
    15361 acgcaggtga ccctcggatt gttcctctcg acggtcgtgt tctgcgcgct gaccctcgga
    15421 tcggtccgca ccgggatcgg atccgcgggc gtcccggcgg tgtcctacgg cgtgtcgatg
    15481 ctgttgggac tgtcctgctt cgtcgctctc gtcatctaca tccaccgggt ggcgacgacg
    15541 atccagtcgc ccaacgtggt ggcgtcggtg gtgcgtgccc tcgaccgctc tctcgacgag
    15601 cgccaggcga tgctcgaggg cgccgagttc agcctcgacg acggcgcggt gcacgcgctg
    15661 cgggagcagg cggcccgcga gggcgacgtg gtggtcgccc acgacagcgg gttcgtgcag
    15721 ggcatcgacc acctccgcat cctcgacgcc gtcgatggcc gcgacgcgta cgtggtgatg
    15781 gagcaccgcg tcgggcagtt cgtggtcgag ggccagccgc tggcgcgggt ggtgccggcg
    15841 tccgcggcgg accggttggc gggtgagctg caccactcgg tggagatcgg accgtggcgg
    15901 acccagcgcc aggacgccga gtacgcgatc taccaggtgg tcgagatcgc catccgggcg
    15961 ttgtcgcccg cggtgaacga cacgttcacg gggatggtgt gcgtcgactg gctcggtgcg
    16021 gcactggcgc ggatgggtcg tgacccggac ggcaacggtg ggctcgaggg ctcaggggga
    16081 cgggtgcgcc tctacatccc acccctgcgg ttcgagcggg cggtgcgcgc cgcgttcgac
    16141 ctgatccgcc aggccggggc ggccaacccg gccatctcga tccggctgct cgacgtgatc
    16201 gccaccacgg cgaacgacgt gcgccccgag caccgcccgg cgctgcgtgc cgcggcggac
    16261 caggtcctgg ccggagcgtc ggcccgaggc ccgatcggac aggacgccga cgacatcgtc
    16321 gagcggcatc tgcggacgat cgcgacgatc gaagcgctcg gatcagggtg atccctccga
    16381 tcggacacga gcggggtcgt tccggccggt tcgcctaccc gacgacggcg cggtcgtcga
    16441 tctcggtggg ggagctgcgt cggacatgtg ggtcgacgat cgacgacgga acggtcgcgg
    16501 gagcgggcgg cggaccggtc gaggtggtgg gtcggacgat gctggtccgt gaccacggcg
    16561 ggatcgcgtt cgtggtcatc cagcaggagg gcgagcgggt ccagctcgtc gtcacccggg
    16621 tcggcgacga aggccttctg gtgctgcccg agcgtggtga cgtggtggcg gcccgcggga
    16681 ccgtcggtcg gacccgcacc ggggagctgt cggtgttcac cgaccggatc acggtcctgg
    16741 ccccgcacct gctgccgccg cccgacaagc accagggccc ccgtgagccg ggtcggcgag
    16801 cccgggagcg tgagctggac ctgatcgcca accccgacag ccgtcggacg ttcgcgatcc
    16861 ggtccaccgt cgtccacgcg ctgcgcagcg agctgcacga ccgtggcttc accgaggtcg
    16921 agaccccggt gttcgcgtcg tcggccggcg gggcggtcgc ccgaccgttc gtcacccacc
    16981 accacgccct gggcatcgac ctgtacctgc gcatcgctcc cgagctgtac ctgaagcgcc
    17041 tggtggtggg cgggatggac cgggtgttcg agatcggcaa ggtgttccgc aacgagggga
    17101 tcgacgccgc tcacaacccg gagttcacga tcctcgaggc ctaccagtgc ccgggcgacc
    17161 agttcgacat gatggacctg gtcgagcagc tggtcgtcgc ggccggccgg gcggccgggt
    17221 cgaccgaggt ggacgtcgac gggaaccgca tcgacctggc ccggccgtgg cggcgggtgg
    17281 ccatggtcga cctggtcgag gacgtgaccg ggcaacgcat cgaccccgcc ggggatctgg
    17341 cggcggccca cgctgcgctc gacgaactgg gggtgacctg ggaggagccc tggggacccg
    17401 gccgttgcct gctcgccgcg ttcgaggagc gcgtggagtc gaccctggtc gaacccacga
    17461 tcgtgcacga ccacccgttg gagacctcgc ccttggcccg ggcgcaccgc agcgtcccgg
    17521 gcgtggccga gcggttcgag gtgttcgtcg ccggccgcga actggccaac gcctacagcg
    17581 agctcaacga tccggtgcag caacgcgagc gcatggagtc ggccaccgcc atcggtgccg
    17641 aggtgtcaga ggtcgacgag gggttcctgc gggcgctcga acgtggcctg gcacccacgg
    17701 gcggactcgg catcggcgtc gaccgcctgg tcatgctgct gtcgggagcg acgtcgatcc
    17761 gcgacgtcat cctgttcccg acccaacggc cggagcggcg tggcggcgag gtcacggcgc
    17821 cccgtccggt gctgccgacg actccgttgc cgacgacggc ccggccgggg gcgccggtcg
    17881 ctgtcgtgcg cctccccggg ctggtccgcg tcgtcgccac gctgaccacc gtcgtcggcg
    17941 tcctcacgat gctcagcgcg ctgccgggtc tgggggtacg ggtcctgcct ctcgacgagc
    18001 tcctctcgcc gctgccccag gtcgtcgacg accgggtcct cgccgtcgga ctcgggctat
    18061 gcatggtgct gctcgccgga cagctgctgc ggggcaagag acgggcctgg aagctggcgg
    18121 tcggactctt tgccctgtcc gcggtgatca gcgtgctccg cggtggcgag gtcgttgccc
    18181 tgggtgcgtc gctcagcatg ctcgtcgtgt tgggcctgac ccgccgtgag ttcaccgggc
    18241 tgcgcgaccc accggcgatc acgcaggtcc tcctcgccgc gccgcggttc ctgctgttcg
    18301 tctacggcta cgggttcctg gcgctgtgga gccaacgcaa ccgcctggaa cccccattcg
    18361 ggtggatccg atcggccgag gcgatcaccg tcggcctgtt cggtgcagcc gggccctacg
    18421 actactcggg tcggttcgcc acctggttcc cggcatcgct ggtggtgctc ggcgtgatcg
    18481 ggctgctcct cctgctctgg ctgctgctgc gcccggccgt cgccgccgcg gtcggcgact
    18541 ccgacgcggc cggcaaccgc gcccgggccg aggacctggt gagggagtgg ggctgggaca
    18601 cgctcgcgcc gttcgccctg cgccgggacc gcagctggtt cttctcctcg gacggccggg
    18661 cgatgatccc ctacgggtac ctgggcggct tcgccctcgg ttccggcgac ccgatcggcg
    18721 acccgtcctc gatcccgctg gcggtcgacg agttcatcga gcactgccgg caccacggct
    18781 ggcagccggc gttcctcgcc gcccgcgaga ttgatgccgc cttctactcc gagcgcggct
    18841 tccgcgactt ctacctgggc gacgaggcgg tcctcgactg tcggaccttc gacctggacg
    18901 ctccgggcat ggccccggtg cgacagtcgg tccgccgcgt cgagggcacc caccgcgtcg
    18961 atttgatcgc cgagtcggag gccgacccgg ctttggtcgc ccgactcaac gagatcagcg
    19021 tccgctggcg caagggcgcc gacgagcggg gctacacgat ggcgatgagc accgaggtgg
    19081 gcgcgtccga ccccggtcga ctgctcgcgg tggcctggcg gctcatgcct ggggaacccg
    19141 ggaccgacgc gaccgccgag gtccccgtcg cgttcctgag gttgatcccg accggcgggg
    19201 gcgacggacc gtacggaagg ggctacacgc tcgacctgat gcggcggctg cccgacgcgg
    19261 ccaacggcat catcgagctg ctggtcgccc ggaccgtggc cgagctcgac cagcggggcg
    19321 tgcgacggct gtcgctcaac ttcgccgcgt tctcccggtt gctcgacgac gacgtccagc
    19381 acacccgggt cgatcgcctg ctgcggcggg tggtcgacgt gctcaacccc tactaccaga
    19441 tccggtcgct gcgcgagttc aacgagaagt tccaacccga gtggcagccc cgctcgctga
    19501 tctacgccga tccgggcgac ctccccaagg tggcgttgcg gtacgcgttc ctcgagggct
    19561 tcgtcgacgt cccgctgatc ggccggctcc tgtccgggcc gtccgagggt ggagcgatcg
    19621 ggccgaggtg acatgctgga cgccgtgagc gacgaacgac ccggaccact ggaccgcatg
    19681 cgggaccccg acgccgaccc ggcccgtgac cgggcccgac tccgggccgc cgccatgatg
    19741 gctggcatgg gcatcgccca cttcctggtc cccggcccgt tccggcggat cgtgccgcgc
    19801 tggttcccgt ggcgacggga ggcggtgatg gtctccggcg tcgccgagat cgcatccgga
    19861 gcactgctgg cgatcccgcg cacccgtcgg gccgggggct ggctcatgct gctcaccgtg
    19921 ctcgccgtgt accccgccaa cgtgcagatg gcgatcgatg cgtcccgggg gcgttcccag
    19981 gtgcgcgtgc cggtgtggct cacctggttg aggttgccgc tgcagctgcc gatgctggcc
    20041 cgggcatgga gcttcacccg ctgagcggac acggtcacga cccttcctcc cgcccggtcg
    20101 cgtccctccc cgggttcgcg gaccgatcgg tcctggagtc ccgcgtcgac gccgcgtcgg
    20161 gtgccgggcg gccgttcggg gcggagcgcc cggtcgggct ggtgcgcgtg agcggacctg
    20221 gtgaccgcgc cgcggagctg gccgacaggc tccgttcagc ggtgcggccc ggcgacacgg
    20281 tcgccgagct cggagccggc tgcttcgcgg tcctctgcga ccggctgggc gaacccgacg
    20341 acctcctcac catcgcccac cgggtggagc agcagctcac cggggtcccg ccgctcgacc
    20401 cccgactgga cgtcgccctg gtcgaccacg ccggcaggga acctgcgagg atcgtcggtg
    20461 acgatcgacc gggccgacgt gcggaggacc ccgaggacct cccgtccggg acgtgggtcc
    20521 ggttcgacga ggcgctgcgg gatcgggcga cgcgccgcga gcagaccgag gcggccctgc
    20581 gtcggtccag cgacggtcgg gacctggtcc tgcactatca accggagcgc gacctcgtga
    20641 cgggtcgtac cgcgggggtc gaagccctcc tgcgctggga gcgcgacggc gtgacggtcg
    20701 gcccggaccg cttcgtcccg ctggccgagc acaccgggct gatcgtgccg atcggcgagt
    20761 gggtcctgcg gaccgcgtgc gaccagctcc tgaggtggcg tcgcgacggg gcggcccggg
    20821 acgcgttcgt ggcggtcaac gtgtcgccgc gtcagctcat gcacgacggc ttcgtcgttt
    20881 cggtccgcga ggccctggcc gatccggggc tcgctcctgg tgacctcgtt ctcgagctga
    20941 ccgagggcgt tctcctcaac gacctggcgc tggcacgcga ccggttgggc gagctgcgcg
    21001 gcctgggggc ccgggtggcg gtggacgact tcggcaccgg ctacgcatcg ctcacctacc
    21061 tgcaccagct gcccatcgac gcggtgaagc tcgaccgcag cttcgtgagc ggcctcggcg
    21121 tcgacccccg cctcaccgcc atcgtgacct cggtgctcgg gttgctgggg gccctcggct
    21181 tgcagtcgat cgccgaaggc gtgtcgagcg ccgcgcaggc cgaacggctg gtcgccctcg
    21241 gctgcgcgct ggcccagggc gagtgggtcg gcacacccgc cccgccggag cagctcgtcg
    21301 accagctgcg ctgagaggcg tcgggcggat cagaagggca gggcggggtg gcccagaacc
    21361 cggtgcggat cgcagcggtc ccgcagggac cgcagcaccg cgcgatcgcc cggaggtgtc
    21421 gtgcggatcc accagtccgc gttgtgcgga ccgatcccgt gctcggcgga gtagctcccg
    21481 tggtggtcgt cgaccgcggt tccgaacacg acccggcggg cgtcctcgcg ctcgctgtcg
    21541 ccgggaggtc cgtcggggaa caccaggttg cagtgggtgc cgccgtcggc ccagtggccg
    21601 aagtccgcca ccatcgcccg cggcacagtg gtcgcgagcc gctcccggac gtcgtcgcgg
    21661 aacgcaacca gctcggaacg ggggatcgag acgtcgaacc cgacgacggt gccggcccgc
    21721 gcgagtccct cggtgatcga gtgtcgcagg tgccacgccc ggtcggtcgg gaccaggacg
    21781 gcgtcatcca ccacgccggc gtcatcgagc gccgccaccg cgtcggcgag cacctccgcg
    21841 gtgccgatgg ggccggccgc ctcgaccagc accgtcacct cgggggcggg cctccggtca
    21901 ccccacggcg agcggaggcc gtcgatgtgg tccaccgcca ggtccagggc cgtgtccgag
    21961 acgacctcgt aggccgacag cagcggcccg agcgcatcct ccaggatccg cagcgcgtcc
    22021 agtgccgcgg cggcgtcgac cggggccagc aggccgcacg ccgactcccg ggggactcgg
    22081 gccagctcga ccgccacccg ggtaacgacg ccgaacgccc cgcccgaacc caccatcagc
    22141 gaggtcaggc gaggtccggt gttgtccttg cgcagccgcg tcaggtcgtc gatcacggtt
    22201 ggaccggacc cgttggtcag ctcgacgtcg gccaggaccg cctcgacgcc gagcacccgg
    22261 cggcgcatgt cgccgtgcgc cagcatccgg gccccgccgg tgtcggtcgc caccatcgct
    22321 cccaggcacg gatcggcccc caggtcgatc ggcagcgaca ggcccagcgg agccaaccgg
    22381 tcgttcaacg tcgagagcct caccccggcc gggacgatcg ccacccggtc atccgcgaag
    22441 acctcgatcg ggtcgaccaa ccgctcggtg ctgaggacca cgatcggtcg agggtcggat
    22501 cccgacggaa cggtcggtgg caccgacgcg ccgaccaggc ccgtgttggc gccctggggc
    22561 agcagacgga acccgtcgag cacggcgtgt cggacgaccg tgcacacctg cgcggtcgtc
    22621 gccgggcgca acaccgccgc ggcgggaccg ccccggccgc gggcgggctc caggtagcgg
    22681 tcgaggacgt cccggcccgc tcgcagcacc gaccccgctc cgagcgcgtc ctctaggtcg
    22741 tcgagcagtc ggtcgtcgag cagtcggtcg tcgagcagcc ggtcgtcgag gggtgtcgcg
    22801 gtctcgccgt cggtctcctc cacgtcgcga caacataggg gtcccagtcc ggccccggag
    22861 gagcgaccca gatgacgacg accgacgcag aggatccgac cgcgccgtac gccggccacg
    22921 agggccgggt gggtcgggtg ttcgccacct cggagccgtg gtggcccgaa cgtcgtcggc
    22981 cggccgaggg cgctcccaac atcgtcgtcg tgctctgcga cgacctgggc tactcggacc
    23041 tcggttgcta cggctcggag atcccgacgc ccaacatcga cgcgatcgcg gccgaggggc
    23101 tgcgctacac cgactaccac tcggcgccga tgtgctcgcc cacccgggcg tcgctgatga
    23161 ccggcctcga gccgcaccgc gccggggtcg gtcacgtggc ccactccgat ccgggcttcc
    23221 cgggctacgc catggagctg accccgttcg cgcccacgct gccggagctg ctgcgagacg
    23281 ccgggtacca gacgctgatg gtcggcaagt ggcacctgac caaggacagc gaggtcaacg
    23341 acgccgggga ccggtcgtcg tggccgctgc agaagggctt cgaccgctac tacgggttcc
    23401 tcgacgcctt caccaacttc caccacccgc accgcctgat cgaggacaac cacgcggtgc
    23461 gcaccgacga gtaccccgag ggctacttcg tcaccgacga cttcaccgac cgggcgatcg
    23521 acatggtgcg cgagtccaag gcgtcggacc cgaccaagcc gttcttcctc tacttctcgc
    23581 atgccgcggt gcacgccccc ttgcaggcca aggccgtcga cgtcgaacgc cacgccgaga
    23641 cctaccgggc cgggtgggac gccatccgcc aggcccgcta cgaccgccag gtcgacctgg
    23701 gggtgatccc cgatggcgcc gagctcgcgc cccgcaacac cgagtccggc gacgacgtcc
    23761 cgccgtggga cgagctcgat gacgaccaga agcggctgtt cgcccgctac atggcggtct
    23821 acgccggtct gatcgacaac ctcgaccaga acatcgggcg gctgcgcggc gcgctcgagg
    23881 agatgggcga gtgggacaac acgctgttca tcttcacctc cgacaacggc gggtcccgcg
    23941 aaggtcagga gcagggcacc tcggcgtact tccggaccct gcactgggag cgcaagggcg
    24001 ccgcggagcc gttcgaggag gacctggccc gacacgacgt catgggcggg ccgaccaccc
    24061 tgccgcacta cccgcggggc tgggcgatgg tgtcgaacac gccgttccgg ctctacaaga
    24121 tcaacgccca ccgcggcggg cactcggttc cgttcgtcgt gtcgtggccc agggcgctgg
    24181 ccgacagcgg tctcgccggc ggcggactgc gtgagcagta cctgcacgtc accgacctgc
    24241 tgccgagctt cctggagctc gccggggtga cccgccccga cgagtggcgc ggcaaccccg
    24301 tgctgccgct cgccggcgag gcggtgttcg gcccgacgat caccgacccc gacgcggtcg
    24361 ggcgcagccg tgacgtcgtg gtcgagaacg agggtcaccg cggctatcgc cgcgacggct
    24421 gggaggtcgt gacccgccac ccggcccgga ccccgttctc tgctgaccac tgggagctgt
    24481 tcgacatggc tgcggacccg acgcagctcc gggacctgtc cgacgagcac cccgatcgtc
    24541 gggacgagct ggtcgaggcg tgggagtccg cggcgtggga gaaccagatc ttcccgatgg
    24601 acgaggggac cggctaccgg ttcctgctgc gtccgccgtg ggtcgagcgc tacgacgaac
    24661 ccgtcgtgct gcacgcgggg atgccgaccc tcgaccgctg gcgctcccag cggctgatcc
    24721 tctggcgcga cgccgccatc atcgcggacg tgacgctggg cgagggtgac cgggggatcc
    24781 tgttcgccca cggcgaccag ggcggcggct actcgctcta cgtcgacgac accggcgagc
    24841 tggtcgcggc gcacaatggc tacggcctgg agggagaggt ccgcggacct gtggtcggtc
    24901 cagggcgtca cgagctcgag ctgcgcatca cctgcccggg cgacgacctc tgggacctgg
    24961 ccgtcgcggt cgacggcgag gccgtcgcgg ccgggaccgg gttccggttg ctgatggcga
    25021 tggcgccgtt cgaggggatc gacgtcggcc tcgaccgccg gtccccggtg tgctggaagg
    25081 tctacgagcg ccacgggtcg ttcccgttca ccgggacgat ccactcggtg cgctaccagc
    25141 cgggtgatcc cgcgccggac agcccgacca acttcctcga ggtgctccgc gactggggcc
    25201 gcagcatgga gtgacccgcc cccgcgtcgc ccccgacccc gctttgtccc gcacagacgc
    25261 ggtgatcacc gcgtctgtgc gtgacaaagc gg
//
LOCUS       DAOQOD010000023        24407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_690_length_24407_cov_13.103811, whole genome shotgun sequence.
ACCESSION   DAOQOD010000023 DAOQOD010000000
VERSION     DAOQOD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 24407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24407
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_24407_cov_13.103811"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PLZ72_04095"
     CDS             complement(<1..1262)
                     /locus_tag="PLZ72_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HNA35516.1"
                     /translation="MTDPVRPRRPLPSGARTVLRAQAEVVAGLLRWIVLGMLVGVLGG
                     LASAVFLVSLRWATEAFHRHGVLLWFLPLAGLAIGLAYHYGGGRSVEGNNLILGEIHD
                     PTDWVPRRMAPMVLIGTIVTHLFGGSAGREGTAIQMSGSLTDAASRLLRLHGADRRIM
                     LVTAIAGGFGAVFGVPLAGAVFALEVQAIGRLRYEALVPCLAASVTGDLVVRGAGIHH
                     TITPHLGQVDLSVGLLLKVAVAGVCFGLVSVLFSMSDHWVKQITGRWVGWAPARPLLG
                     GVVVVAFTFAVGNRLYNGLSEGLVEISLAGGSVPTWAFALKLLFTAVTLGSGFVGGEV
                     TPLFVIGATLGATLAGPLGVPPALLAALGFVAVFAGAANTPLACTIMGVEMFGAGAVP
                     YFAVACVISYVFSAHQGIYRTQQSSGPKV"
     gene            complement(1280..2560)
                     /gene="serS"
                     /locus_tag="PLZ72_04100"
     CDS             complement(1280..2560)
                     /gene="serS"
                     /locus_tag="PLZ72_04100"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNA35517.1"
                     /translation="MLDVRLFRNDPDAVRVGLARRGEDPGAVDEVVRLDAELRNLTAR
                     RDGLRAEIRTLSNEVGKLFREKRQDEAAELQEQSRILGAEEKALDVGADEMAAELREK
                     LLRIPNLPSPDAPDGLGEDDNVVVRVEGFDADRYADHQRVSHWEIGEQLGILDVERAV
                     KLSGSMFVMYRGAGAALVRALCQLALDRNVDLYEEIRPPTLVRTDTMVSTGHLPKFEE
                     EAYHLERDDLWAIPTAEVPLTSLGRDEIVDEADLPRRFCAHTSCFRREAGSAGKDTRG
                     LLRVHEFDKVEILAYSTPAQAAGLHSEILDRAESTIRDLGLAYRVLDLCAGDLGASAR
                     RTFDIEVFAPGADRWLEVSSVSWFGDYQARRANLRYRPAEGKGTEILHTLNGSALAVP
                     RVWAAIVETYRRTDGRVDVPDLLQPYLRGLTVIG"
     gene            2628..4088
                     /locus_tag="PLZ72_04105"
     CDS             2628..4088
                     /locus_tag="PLZ72_04105"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA35518.1"
                     /translation="MADALPLTDAPDDDAVTALLAEALQAALAEHHVPGAVAGVVSND
                     VRHVTGAGVGHHELPRAVDGDTLFQVGSITKTLTSAAVLALVQDGRLSLDDPVEVHLP
                     GLGEATGLDTEAITVEVALAHTAGFDGDHLFVEGARDGLATLATARRFFHPGTGWSYN
                     NAGFSIVGEVIAAAAGVPFESFVRERLLDPLGMATAGFRADDVITRDVAAPHWMFDGA
                     AYVLRGIGWQPGWELTPLDRAAGGLVASVNHLLVWCRFQWSGTGLDGTRLLTDDSLRR
                     LHHPVTAEVRPGESVALDWFVHTHEVGPEHAPARGSASPDPSGPVTTIEHGGTTVGYI
                     SDLVIAPDQRLGIVVLTNATNGAAVTQSVRRTMLAELLGVVEADPVPDPDLRPDLDRL
                     TGVYEHAFATLTVTAGDDPGTVVVTPSPRNVDGWQPPVTSPVTFGFSSPTDIVSLDHP
                     APVKVAHFDPDGDRAQWLLWEHRRAPRTGDVPGAPT"
     gene            4085..4726
                     /locus_tag="PLZ72_04110"
     CDS             4085..4726
                     /locus_tag="PLZ72_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNA35519.1"
                     /translation="MNVVFDLDGTVWDSEPGILACMVHTLDALGLQVPTDQVLRSHIG
                     LPLPLMLEMVGVPAGHVEAGVRLYRDRYVRTGVYEASLYDGAVDMLDAVAAAGHTLAT
                     ATSKGEGPTRTMLDHFGLTDRFTVIGAASMDGTSTSKTDVLARTLAGLGDPAPSDCVL
                     VGDRDLDVLGAAAHGIDCIGATWGYGTVGELQAAGAWAVAERPSDVPGLVARR"
     gene            4738..5742
                     /locus_tag="PLZ72_04115"
     CDS             4738..5742
                     /locus_tag="PLZ72_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35520.1"
                     /translation="MTTTGSPTTGSGGEAVVVEDLVIRYGELTAVDGVSFTARAGQVT
                     TVLGPNGAGKTSTVEHLEGYRPAAGGRTRVLGLDPITDHRRLSHRVGVMLQDGGIPTA
                     IRPAELLTQYAGFFERPLDPAELLERVGLSHRAGTAFRRLSGGEQRRLSLALAIIGRP
                     DVVFLDEPTAGIDLEGRDAVREVVASLSEDGVAVVMTTHDLDEAERCSDHVVIIDRGT
                     VVAAGSPAELVRPSDSDHILFGAPDELPVDDLAAHLGVPVRRVSRGEFRVETDPSPAN
                     VAAVTAWLAERDLPLQDLRAGRQRLDDVFRRLTAHDRGPDAGDPGGPAARRRGRGRRR
                     "
     gene            5739..6470
                     /locus_tag="PLZ72_04120"
     CDS             5739..6470
                     /locus_tag="PLZ72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35521.1"
                     /translation="MRAFRSQLRTELVLGARQGEQLLVNLGIPLLILVFFSTVDVLPH
                     GDVDEPIDYLAPAVLALAVMSSSLVSLGIGTGFERHYGVLKRLGASPLGRGRWVAAKI
                     TSVLVTEVIQWAVLIAVAVLLGWSPPAAGWAPAVLGALLGTAAFGGLGLLLAGTLPGV
                     GTLAVANGLYLVLLLTGGMVVPLSELPSGLATAARALPAAPLTEVITGSLGGGGVDTW
                     AWVSLTAWAVVAPVAAALLFRWSAE"
     gene            complement(6540..6962)
                     /locus_tag="PLZ72_04125"
     CDS             complement(6540..6962)
                     /locus_tag="PLZ72_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNA35522.1"
                     /translation="MSTTVEELDDDSPVHPGEHHTHGPSTSTYFTIFWVLVAITALEV
                     STYFWSSWFGDGSHYPAVVLLVVMMVVKFILIASFFMHLRFDSKLLSRIFYFGLLVAM
                     MVYVAALATMNIFTDNGNPWFNDPPPAVTTTTVAPAGG"
     gene            complement(7018..7647)
                     /locus_tag="PLZ72_04130"
     CDS             complement(7018..7647)
                     /locus_tag="PLZ72_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNA35523.1"
                     /translation="MADIALEPVEDLEFPDDTHATSTGISNTKMAMWVYLASDCLLFG
                     GLISTYLLYKNRPGSIPGLAGSPLKASELFNIPFTSMTSFILLMSSLTMVLAVNAVMG
                     GDHKRMRLWLSATCVLGGLFLAGQVYEFTEFVREGLGYTSNVSASAFYTLTGFHGAHV
                     AIGIIMLFSAVGYSFRKQLQAEAVEVLGLYWHFVDIVWVVIFAIVYLIP"
     gene            complement(7640..9733)
                     /gene="ctaD"
                     /locus_tag="PLZ72_04135"
     CDS             complement(7640..9733)
                     /gene="ctaD"
                     /locus_tag="PLZ72_04135"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNA35524.1"
                     /translation="MALVEDHPPLELGSGAATVSKPLGVFARPTATHGWKSWLFTVDH
                     KKIGIMYGVAAFFFLLVGGSEALLIRTQLAVPGAKLLSADVYNQVFTMHGVTMIFLAV
                     MPMQSAFANYLLPLQIGARDVAFPRMNALSLWIWLSGAIFFNTSWFLGGGANGGWFNY
                     APNSGVTFSPGHGIDFYAVGLIIMGIGSTVSAINLTVTVLNMRSPGMTLMRMPVFTWM
                     AFVTQVLLVFALPVITVALFLLMFDRLFDATFFDASKGGDPFLWQNLFWIFGHPEVYI
                     MILPSFGIVSEIIPVFSRKPIFGYPFMVGSGIAIGFMGWGVWAHHMFASGIGTLSVLA
                     FSLATMFIAVPTGVKILNWMATMYGGRIRLSTPMLFSIGLVAMFTIGGLSGVTHAFAP
                     ADAQQTDTYYIVAHFHYVLFGGALLGLFGGMYFWWPKMFGHFLNEKMGKWNFWIMLVG
                     FNLTFGPMHILGLQGMMRRTYTYTEGQGFNFWNLIATIGAFTIAVSLILFMVNVALSY
                     RRHKANPVDPGPDPWDARSPEWMIPSPAPEHNFDELPVVEEVDEFWHRKYGHDENGRL
                     VRIARTEDVVQKGDATDVHLPSPSYWPLVMACGFPFVGYGLIFNYWWLVPGVLLIILG
                     IFGWVFEPSTDPSAGHHHDDDYPTDDRPDAVDGSEDAPTDAEAGDGEVDGSSDDADER
                     TLEPAMASTEETTVG"
     gene            complement(9765..10967)
                     /gene="coxB"
                     /locus_tag="PLZ72_04140"
     CDS             complement(9765..10967)
                     /gene="coxB"
                     /locus_tag="PLZ72_04140"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNA35525.1"
                     /translation="MSERLGMRNKRNAAIAGIVSLVVMTICLWLIVRNIGDPAMPLNT
                     LAPAGKQNWEIFNLVKPVFFIAGLVFLAVEAGVIWMAVRFKRNKDDVDGVGEPTQTHG
                     NTTLEIGWTLVPALVLAILAVFNVKTVLEIEKSENPIEVRVIGQQWWWEYRYDTNNDG
                     TVDIITATQVVVPVGRDVKMKLQSNDVIHSFWIPAINGKLDAVPGRTHTLIMTADKPG
                     IYQGQCTEYCGLSHGVMRMELKALPPAEFDKWVDAMTSVPDQPTDPKAIAGQELFVSQ
                     CARCHQVDGLTPESSPPFKYSKLPDPEYGQTVDSTLTSKNAPNLTHLMMRQTFAGSLL
                     PLYQGKAAESVTAVPDGVPDRSNLKDWLRDPESVKPMNPENNQGMPNLQLSETQIDQL
                     VAYLVTLK"
     gene            complement(10960..11853)
                     /locus_tag="PLZ72_04145"
     CDS             complement(10960..11853)
                     /locus_tag="PLZ72_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35526.1"
                     /translation="MITRGSKFFYAAAVVGFVTAIVYGFLTGAAAHSGVIGVFQDGDV
                     VNSIVGPLTFGWKGWVGDHVGYSVLMGFAAVMAALGGFSTAFRDGSADVLVAMDGRDP
                     ATATNPPVLTPVGLSYWPMAGAFGVAVSVVGLAVDTTLFWVGVVLLAIVMVSWTVRAW
                     AERATGDQGANRELRHDLMDPLEVPVLSVLTLAVIVLCISRLLLAIPQEVAVYMIIMA
                     AIIVFVMAFILASRPELTRTVVVSVLLVGGVVIIAAGIAGGVAGEQTGEEHGAGIAPT
                     VVVDSASGSGAVDTLTLLAGE"
     gene            complement(11861..12433)
                     /locus_tag="PLZ72_04150"
     CDS             complement(11861..12433)
                     /locus_tag="PLZ72_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNA35527.1"
                     /translation="MATAALPAPLQPRRRELIFGTAFATAGVVAVMATLIGYYLAARN
                     AGGADWFSANTIPLTQPNMQALTLAMSVVTMQWAVYAIARDDRTHTYLALGITLLLGA
                     AFVNQSIFLFKQAAVTIEQPEGPLFYAVTVGSLLMVVAGMIFIALMAFRALGGQFSSR
                     QPDGIAAAAVFWYASCAVYAVVWVAVYIMK"
     gene            complement(12433..13992)
                     /locus_tag="PLZ72_04155"
     CDS             complement(12433..13992)
                     /locus_tag="PLZ72_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNA35528.1"
                     /translation="MALTETRSDTDATSAGDRARRAPSAVEQLIGSGDHTSIGRLFIG
                     FGFALASLSLTLWALTGVDALTDNGFLGSRPAQLAASAQVALLFLGIVPLLLGVAIAV
                     VPLQLGSPSIAFPRAATLSFWTWLLSSGIFAVSIALDGGVLGGDETAAKLGNVSLGAV
                     LVALSLGAVCVATTVMAHRPLGMRLAWVPGLSWASLVSAALLLVTLGSAIAHTVLGQV
                     GRMGPAALATNFTDGLGWLLRGPAVFVFAIPVLGIAIDVVVTASGRRFAHTDVLQLIV
                     GLYAILAFGAWAQLPSALNTALWTLFAFGITVPILGLLGAIGDSLRQGKPVVSPALIL
                     SIVSVLLLMGAAVTGWVQSLSLAGQGTLFGASPATLASAQTAFVAGAALLGGAAASLH
                     WSPLLWGGPGNSVEGRLTVPLLVLGGGLLGTTLLVQSVVQLDGETTAFQIFGLLVAVG
                     AGLLALGGLSALVAAMRATEDPAGEPVQGLTLEWSFPNPAGGGIGLPELARVTSPYPL
                     LDAREGTEDKP"
     gene            complement(14124..15041)
                     /locus_tag="PLZ72_04160"
     CDS             complement(14124..15041)
                     /locus_tag="PLZ72_04160"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNA35529.1"
                     /translation="MAAPAISSPTSIGAKLATTVGAYVALTKPRIIELLLVTTVPVMV
                     VADQGVPPLWLMAAAVIGGTLAAGGANAINMYVDRDIDSVMERTQGRPLVTGAVTPRN
                     ALVFAVTIEVVAFVFLWQVVNLLSAVLAVSGCLYYVFIYTLWLKRSSRQNIVIGGAAG
                     AVPVLVGWSAVTDTVSISAIVLFGVIFFWTPPHFWALAIKYKDDYARADVPMLPSVAS
                     HRQTTDQILAYTVVVWALTLLFGVTAEMGLLYWATAIVLGAVFTWMAVDLRRTESPAR
                     AIRVFTFSITYITVLFGVMALDVIIRNGW"
     gene            15132..16094
                     /locus_tag="PLZ72_04165"
     CDS             15132..16094
                     /locus_tag="PLZ72_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNA35530.1"
                     /translation="MTLEPRTYRRIVAIALALLCAIVVTGAAVRLSGSGLGCDDWPNC
                     SSERVIEFGNPNQTIEQVNRLFTGLVSLSVAAAVLGSIRRRPYRRDLVVLSWGLVAGV
                     IGQIVLGGITVLVGLHPAAVAGHFLLSMILVADAVVLLWRSGQPDGPRRPQVAPAELW
                     LSRVAVVLMVGLMFTGPAVTGTGPHAGDAESPRFGWFLPDVARIHSINMWIFLAVVVV
                     LLVRLARSGAPRPVLHRAQALLVVIVAQGAIGYTQYEMGIPPWLVALHIAGATAVLGV
                     TVWFHLGLSAPVTAPDGIDGDGLDPVRTDDKDGTVDRAGSSSSA"
     gene            16104..16394
                     /gene="clpS"
                     /locus_tag="PLZ72_04170"
     CDS             16104..16394
                     /gene="clpS"
                     /locus_tag="PLZ72_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNA35531.1"
                     /translation="MSTPAPIDVVEPDVSDLVDLDTPWVVLVWNDPINLMSYVTFVLQ
                     KLFGYSKEEATTLMLQVHHDGKAVVSTGSRTEAERDVFRLHEHGLWATMQQD"
     gene            16401..16871
                     /locus_tag="PLZ72_04175"
     CDS             16401..16871
                     /locus_tag="PLZ72_04175"
                     /inference="COORDINATES: protein motif:HMM:NF020989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HNA35532.1"
                     /translation="MARFRPAVTADDGDTYTISLGPNERDLIRSVTEQLKELLVETDG
                     PVLRRLFPPPYGDDDERNEGYAALARPELIENRLASLDVVIGSAEATTLDEDSMAAWM
                     RSINDVRLVLGTVLDITDDSEEVEVDDSNVATYQAYEVLGMLLETIVAARSSRL"
     gene            16957..17029
                     /locus_tag="PLZ72_04180"
     tRNA            16957..17029
                     /locus_tag="PLZ72_04180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16991..16993,aa:Glu,seq:ttc)
     gene            17048..17125
                     /locus_tag="PLZ72_04185"
     tRNA            17048..17125
                     /locus_tag="PLZ72_04185"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17083..17085,aa:Asp,seq:gtc)
     gene            17165..17237
                     /locus_tag="PLZ72_04190"
     tRNA            17165..17237
                     /locus_tag="PLZ72_04190"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17198..17200,aa:Phe,seq:gaa)
     gene            complement(17412..18812)
                     /locus_tag="PLZ72_04195"
     CDS             complement(17412..18812)
                     /locus_tag="PLZ72_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNA35533.1"
                     /translation="MADTPTPAYSVRIRVRMENRPGMLGELAIAIGAVGANISALQGF
                     EVKTASLDEDVVVNCASEAHQEQVRAAVEGIDGIQILEFEDRTFHMHEGGKIEVLSLA
                     PVRDVEDLSMAYTPGVARVCMEIAKNPPAAHRYTIKKNTVAIVSDGTAVLGLGDIGPE
                     GAMPVMEGKALLFKHFAGVDAFPICLNTKDPEEIIETVLRLEPTFGGINLEDIAAPGA
                     FEVEERLDECMDIPVFHDDQHGTAIVTLAALENALKIVDKDMADLKVVVAGVGAAGVA
                     VSKILMNAGVRNVIGADRQGAVHTSREGLNRSKQWFAENTNPEGLSGSLTQIMPGADV
                     FIGLSGPNLIERSDVERMASNPIVFAMANPDPEIRPELIQDVAAVIATGRSDFPNQIN
                     NVLAFPGVFRGALDAGARRITENMKVAAARAIASTVEPDELAPDFIIPSVFNPRVSVA
                     VAEACAEAARADGVCR"
     gene            complement(18869..19441)
                     /gene="pyrE"
                     /locus_tag="PLZ72_04200"
     CDS             complement(18869..19441)
                     /gene="pyrE"
                     /locus_tag="PLZ72_04200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNA35534.1"
                     /translation="MTLPGIPADVHRALVAHLLEHSVRTGDFTLKSGRRSSWFIDSKQ
                     TACDPDGVLLMAGAALAVIPDDATAIGGLTVGADPVAYGVAAVAATRGRRLRSFTIRK
                     EAKDHGVTGRLAGPLRAGDRVAITEDTVTRGTSLFEAVEVVRELGAEPVLIMPLVDRG
                     GTCAAMAAEAGIAYRPLVTAPDLGFAYEGP"
     gene            complement(19466..20611)
                     /locus_tag="PLZ72_04205"
     CDS             complement(19466..20611)
                     /locus_tag="PLZ72_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35535.1"
                     /translation="MTDFVAPRAHGSLSSWDDYPVHQAAETVRHATTSDRNFYDRYYF
                     NCHADDGSAFLIFGLGQYPNLAVQDAFVCVVSGDDHRVVRASRELGDRMDTSVGPLRV
                     EVIRPLHELRIVCEPNEHSDVSVDLRWVGSIPAFEEPRQYVRRHGRTLFDSMRLAQTG
                     RWSGTIRSGGTTFDVTPDRWWGTRDRSWGIRPVGEEEPAGIRTEGQMTGMWNYAPMQF
                     EDHSILYILNEHDDGHREHEEAVRIWNDPDREPEWLGRPEHDAVITPGTRRIESSTLS
                     FPDAPGGAIDVAVEPLMDCWLMLGTGYGIEADWRHGMYQGPLVVQGVEIDYRRDADRL
                     FGLVDQVARFTQTGGVADGAVGSGLHEFFFIGDYPRYGLRGWDPRPT"
     gene            complement(20726..21763)
                     /gene="purM"
                     /locus_tag="PLZ72_04210"
     CDS             complement(20726..21763)
                     /gene="purM"
                     /locus_tag="PLZ72_04210"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNA35536.1"
                     /translation="MGETYAGAGVSIDAGEDAVDRIRSHVGSTRRPEVLGGIGGFGGL
                     FAFDAAGYREPVLVSTTDGVGTKALIATATGRYDTIGLDLVAMCVDDLVCQGAEPLFF
                     LDYISIGKVDPDVIESLVAGVAEGCRQAGCALVGGEMAEHAGVMEPGEFDLVGFAVGA
                     VERSAMITGESVTPGDVLVALPSPGLRSNGYTLARRLMFDVAGRGLDDPAWDDADAPT
                     VADELLRPSVIYAPAVRALIDAVPVHSVAHITGGGLPGNLNRVIGDAADAVVDPTSWE
                     APRIFSELQRIGDVDTDEMRRVFNLGIGMVVAVPPEVVADAASVLDGHGLAAAVIGEV
                     VAGSGRVRFTS"
     gene            complement(21756..23411)
                     /gene="purF"
                     /locus_tag="PLZ72_04215"
     CDS             complement(21756..23411)
                     /gene="purF"
                     /locus_tag="PLZ72_04215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNA35537.1"
                     /translation="MLPASNDDGPHAPEPDSRPPGDGVAGGPDLEVDPEGGKPREACG
                     VFGVYAPGAPVAHLTYLGIYALQHRGQESAGIASSNGDHLTVVKEMGLVSNVFDDRTL
                     AVLDGDLAIGHTRYSTTGSSMWKNSQPVFRDVVHQQFALAHNGNLVNTAQLVDDAGML
                     PGTVSSDTDAMAEVIALECERLGPGDDTLERALVTVLPSFEGAFSLVLMDRNRVIGVR
                     DPRGFRPLCLGKLDSGWVLASETPALDVVGAHFVRELDPGEMVVIDAAGVRSVRPFDD
                     SSIDPKLCLFEFVYFARPDSQLYDRNVHQARVRMGEQLADQAPVDADMVMGVPESGIP
                     AAEGYARRSGIPFGQGLVKNRYIGRTFIAPSQEMRALGVRMKLNPLRDNIEGRRLVVV
                     DDSIVRGTTTKAMVAMLREAGAREVHLRVSSPPYRWPCFFGMDTGTRGELLAANLEVD
                     EIRRYLNVDTLAYLTLDRLIDAIGAPGAGFCDACLTGVYPVEIPVEIGKGVLDGAAPV
                     AREGLSEMGSVLLADAGVLPSVDAGSLAAETGSGPADGTVTDG"
     gene            complement(23478..>24407)
                     /gene="gluQRS"
                     /locus_tag="PLZ72_04220"
     CDS             complement(23478..>24407)
                     /gene="gluQRS"
                     /locus_tag="PLZ72_04220"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNA35538.1"
                     /translation="VLTGRFAPSPSAPLHLGNLRTALLAWAAARSRGGRFLIRIEDLT
                     TSAGDERTAAVAADQLADLAAVGITHDGEVVRQSERRHLYDAAIARLVDAGLTYPCYC
                     TRREIRDAAVAPHGPDPDGAYPATCRHLSATARHALERDGRRPALRLRADGARVTVTD
                     EMVGTLSVVVDDLVLRRADGEVAYNLAVVVDDADQGVTQVVRGDDLWETTPRQVLLQQ
                     LLDLPTPEYLHVPLVLAADGRRLSKRDGAVTLADRTELGEDVAEVVGMLAGSAGLAEP
                     GERLDAATVADRFALPRLVRDPWVFRDGRAARA"
BASE COUNT         3782 a         8425 c         8759 g         3441 t
ORIGIN      
        1 accttgggac ccgacgactg ctgcgtgcgg tagatgccct gatgggccga gaacacgtag
       61 ctgatcacgc aggcgacggc gaagtacggc acggctccgg cgccgaacat ctcgactccc
      121 atgatcgtgc aggccagcgg ggtgttggcc gccccggcga acacggccac gaacccgagg
      181 gcggccaaca gcgccggcgg cacaccgagc ggcccggcca gggtggcgcc gagggtggcg
      241 ccgatcacga acagcggggt gacctcgccg ccgacgaacc cggagccgag ggtgaccgcg
      301 gtgaacagca acttgagcgc gaacgcccag gtcggcaccg aaccgccggc caggctgatc
      361 tcgaccaggc cctccgacag cccgttgtac aggcggttgc cgacggcgaa cgtgaaggcg
      421 accaccacca cgccgccgag gaggggacgg gccggcgccc agcccaccca gcggccggtg
      481 atctgtttca cccagtggtc cgacatgctg aacagcaccg acaccaaccc gaagcacacc
      541 cccgcgaccg cgactttcaa caggagtccc accgacaggt cgacctgccc gaggtgcggc
      601 gtgatcgtgt ggtggatccc ggccccgcgc accaccaggt cgccggtcac cgacgcggcc
      661 aggcacggga cgagcgcctc gtagcggagc cggccgatcg cctgtacctc gagagcgaac
      721 accgcgcccg cgagcggaac gccgaacacc gccccgaacc cgccggcgat cgcggtcacc
      781 agcatgatcc gtcgatccgc gccgtgcagc cgcaggaggc gcgacgcggc gtcggtcagg
      841 ctgccgctca tctggatggc tgtgccctcg cggccggcgg agcctccgaa gaggtgcgtg
      901 acgatcgtgc cgatcagcac catcggggcc atccgccgcg gcacccagtc ggtcgggtcg
      961 tggatctcgc cgaggatcag gttgttgccc tcgacggacc ggccgccgcc gtagtggtag
     1021 gcgagaccga tcgccaggcc ggccagcggc aggaaccaca gcagcacgcc gtggcggtgg
     1081 aacgcctccg tcgcccatcg cagcgacacc aggaagaccg ccgaggccag ccctccgagc
     1141 acaccgacca gcatcccgag cacgatccag cgcagcagac ccgccaccac ctcggcctgg
     1201 gcacgcagca ccgtgcgggc tccgctcggc agcggccgtc gtggtcggac gggatcggtc
     1261 acgtcacggg gccatcacgt caaccgatga ccgtgagtcc ccggaggtac ggctggagca
     1321 ggtcggggac gtcgacccgc ccgtcggtac ggcggtaggt ctccacgatc gcggcccaga
     1381 cccgggggac ggccagggcc gaaccgttga gggtgtggag gatctcggtg cccttgccct
     1441 ctgccggccg gtaccgcagg ttggcccgac gggcctggta gtcgccgaac cagctgaccg
     1501 agctgacctc gagccagcgg tcggcgccgg gtgcgaacac ctcgatgtcg aaggtgcgtc
     1561 gcgccgacgc gcccaggtca ccggcgcaca ggtccaggac gcggtaggcc agtccgaggt
     1621 cccggatggt cgactcggcc cggtcgagga tctcggagtg cagcccggcc gcctgggccg
     1681 gcgtcgagta ggcgaggatc tcgaccttgt cgaactcgtg cacgcgcagc agtccgcgtg
     1741 tgtccttgcc ggcggagccg gcctcgcgac ggaagcagct ggtgtgggcg cagaagcgac
     1801 ggggcaggtc ggcctcgtcg acgatctcgt cgcggcccag cgaggtgagc ggcacctcgg
     1861 cggtggggat ggcccacagg tcgtcgcgct cgaggtggta cgcctcttcc tcgaacttgg
     1921 ggaggtgacc ggtcgagacc atggtgtcgg tgcgcaccag cgtcggtggc cggatctcct
     1981 cgtacaggtc gacgttgcgg tcgagcgcca gctggcacag cgccctcacc agcgcggcgc
     2041 cggccccgcg gtacatgacg aacatcgatc ccgacagctt gacggcccgt tccacgtcga
     2101 ggatgccgag ctgctcgccg atctcccagt gggacacgcg ttggtgatcg gcgtagcggt
     2161 cggcgtcgaa gccctcgacg cggaccacga cgttgtcgtc ctcgccgagg ccgtcgggcg
     2221 cgtcgggcga cgggaggttc gggatgcgca gcagcttctc gcgcagctcg gcggccatct
     2281 cgtccgcgcc gacgtcgagc gccttctcct cggcgccgag gatgcggctc tgctcctgca
     2341 gttcggcggc ctcgtcctgg cgcttctcgc ggaacagctt gccgacttcg ttggacagcg
     2401 tgcggatctc ggcgcggagc ccgtcgcgac gcgcggtcag gttgcgaagc tcggcgtcga
     2461 ggcggacgac ctcgtcgacg gcgcccgggt cctcgccacg gcgggcgagc ccgacgcgca
     2521 ccgcgtcggg gtcgttgcgg aacaggcgca cgtcgagcat ccccgcacgg tagcggcgcc
     2581 cgcctcacgc ccccgaaccc gttggtgccc ctggctaggg tgccgccatg gctgacgccc
     2641 tcccgctgac cgacgctccc gacgacgacg cggtgaccgc gctgctggcg gaggcgttgc
     2701 aggcggcgct cgccgagcac cacgtgccgg gggcggtcgc cggcgtggtg tcgaacgacg
     2761 tccgccacgt caccggcgcc ggggtcggtc accacgagct gccccgggcg gtcgacggcg
     2821 acaccttgtt ccaggtgggc tcgatcacca agaccctgac ctccgccgcg gtgctggccc
     2881 tggtccagga cggtcgcctc tccctcgacg acccggtcga ggtgcacctg cccggcctgg
     2941 gcgaggcgac cggcctcgac accgaggcga tcaccgtcga ggtcgcgctc gcccacaccg
     3001 ccgggttcga cggcgaccac ctgttcgtcg agggcgcgcg ggacggcctg gcgacgctgg
     3061 cgacggcgcg tcggttcttc catcccggga cgggctggtc gtacaacaac gcgggcttct
     3121 cgatcgtcgg cgaggtgatc gcggctgcgg ccggtgtccc cttcgagagc ttcgtgaggg
     3181 aacggctcct cgatcccctg ggcatggcga ccgccgggtt ccgggccgac gatgtcatca
     3241 cccgcgacgt cgccgcgccg cactggatgt tcgacggcgc cgcgtacgtg ctgcgtggca
     3301 tcggctggca gcccggctgg gagctcaccc cgctcgaccg cgcggcgggc ggtctggtgg
     3361 cgtcggtgaa ccacctgctc gtgtggtgcc ggttccagtg gagcggcacc ggcctcgacg
     3421 gcacccggct gctcaccgac gactcgctcc gacgactgca ccacccggtc accgccgagg
     3481 tccgccccgg ggagtcggtc gcgctcgact ggttcgtcca cacccacgag gtcggccccg
     3541 agcacgcgcc ggcccgcggg tcggcatcac cggacccgag cggaccggtg acgacgatcg
     3601 agcacggcgg cacgacggtc ggctacatca gcgacctggt catcgccccc gaccagcggc
     3661 tcgggatcgt ggtgctgacc aacgccacca acggcgcggc ggtcacccag tccgtgcgcc
     3721 ggacgatgct ggccgagctg ctcggagtcg tggaggccga cccggtgccg gacccggacc
     3781 tgcgcccaga cctcgaccgg ctgaccggcg tgtacgagca cgcattcgcc accctcaccg
     3841 tcaccgccgg cgacgacccc ggaaccgtcg tcgtcacccc gtcgccccgc aacgtcgacg
     3901 ggtggcagcc cccggtcacc tcgccggtga cgttcgggtt ctcgtcaccg accgacatcg
     3961 tcagcctcga ccatcccgcg ccggtgaagg tcgcccactt cgaccccgac ggagaccggg
     4021 cccagtggct gctgtgggag caccgacggg caccccgcac cggcgacgtg ccgggagctc
     4081 cgacgtgaac gtcgtgttcg acctcgacgg cacggtctgg gactccgagc ccgggatcct
     4141 ggcgtgcatg gtccacaccc tggacgcact cggcctccaa gtgccgaccg atcaggtcct
     4201 gcgctcccac atcgggctgc cgctgccgct gatgctcgag atggtggggg taccggccgg
     4261 gcacgtcgag gccggcgtcc gtctgtaccg ggaccgctac gtgcggaccg gcgtgtacga
     4321 ggccagcctc tacgacggag cggtcgacat gctcgacgcc gtggccgcgg cgggtcacac
     4381 cctcgccacc gcgacgtcca agggcgaggg tccgacgcgc accatgctcg accacttcgg
     4441 tctgaccgac cggttcacgg tgatcggcgc cgcgtccatg gacgggacga gcacctccaa
     4501 gaccgacgtg ctcgcccgca ccctggccgg cctcggagac cccgcaccct cggactgcgt
     4561 gctcgtcggc gaccgtgacc tggacgtgct cggcgcagcc gcccacggga tcgactgcat
     4621 cggcgcgacc tggggctacg gcacggtcgg cgagctgcag gccgcgggag cctgggcggt
     4681 ggcggagcgc ccctccgacg tgcccgggct cgtggcccgc cggtagcgtc gctcgacgtg
     4741 accacgaccg gatcgcccac caccggctcc ggaggcgagg ccgtcgtcgt cgaggacctg
     4801 gtcatccgct acggggagct caccgcggtc gacggcgtgt cgttcaccgc tcgggccggg
     4861 caggtcacca ccgtgctcgg ccccaacggc gccggcaaga cctcgaccgt cgagcacctc
     4921 gagggctacc gccccgcggc aggcggccgg acccgagtgc tcggcctcga cccgatcacc
     4981 gaccaccgac gcctcagcca ccgggtcggg gtgatgctgc aggacggcgg catccccacc
     5041 gccatccgtc ccgccgagct gctgacccag tacgccggat tcttcgaacg gccgctcgat
     5101 ccggccgagc tgctggagcg tgtcgggctg tcgcaccggg ccggtaccgc attccgacga
     5161 ctgtcgggcg gcgagcagcg ccggctgtcg ctggcgctgg cgatcatcgg gcgtcccgat
     5221 gtggtgttcc tcgacgagcc caccgccggc atcgacctcg agggccgcga cgccgtgcgc
     5281 gaggtggtgg cgtcgctgag cgaagacggg gtggcggtgg tgatgacgac ccacgacctc
     5341 gacgaggccg agcggtgctc ggaccacgtg gtgatcatcg accggggcac cgtcgtcgcc
     5401 gccgggtccc cggccgagct ggtccgcccc agcgactccg accacatcct gttcggcgcg
     5461 cccgacgaac tccccgtcga cgacctggcc gcccacctcg gcgtcccggt ccgtcgggtg
     5521 agccggggcg agttccgcgt cgagaccgac cccagcccgg cgaacgtggc cgcggtgacc
     5581 gcgtggctcg ccgaacggga cctgccgctg caggacctcc gggccgggcg gcagcgcctc
     5641 gacgacgtgt tccgccgcct gaccgcccac gaccgcgggc ccgacgccgg agatccgggc
     5701 ggtcccgccg cccgacgacg tggacgaggg aggcgacggt gagagcgttc cggtcccagc
     5761 tgcgcaccga gttggtgctc ggagcccgcc agggcgagca gctgctggtc aacctcggga
     5821 tcccgctgct gatcctggtg ttcttctcga ccgtcgacgt gctcccccac ggcgacgtcg
     5881 acgagccgat cgactacctg gccccggcgg tgctcgcgtt ggccgtcatg tcgagctcgc
     5941 tggtgtcgct cggcatcggc accgggttcg agcgccacta cggcgtgctc aagcgtctgg
     6001 gggcatcgcc gctcggacgg gggcggtggg tggcagccaa gatcacctcg gtcctggtta
     6061 ccgaggtgat ccagtgggcg gtgctcatcg ccgtcgcggt gctgctcggt tggtcgccgc
     6121 cggcagcggg ctgggcgccg gcggtcctgg gcgccctgct cgggaccgca gcgttcggcg
     6181 gtctcggact gctgctcgcc ggcacgctgc ccggcgtcgg aaccctggcc gtggccaacg
     6241 gcctctacct ggtgctgctg ctgaccggag gcatggtcgt ccccctgtcg gagctgccga
     6301 gcggtctggc cacggcggcg cgggcgctcc ccgccgcccc gctcaccgag gtgatcaccg
     6361 gctccctcgg aggaggcggg gtggacacct gggcgtgggt gtcgctgacc gcatgggcgg
     6421 tcgtcgcccc cgtcgccgcg gcgctgctgt tccgctggtc ggccgagtag cggtcccggg
     6481 gaacgcggag aggccccgcc tgagcggggc ccctcggtcg cagatcgtgt cgggttcgat
     6541 cagccgccgg cgggggcgac cgtcgtcgtg gtcaccgccg gcggcgggtc gttgaaccag
     6601 gggttgccgt tgtcggtgaa gatgttcatg gtcgccagag cggccacgta gaccatcatc
     6661 gccacgagca gaccgaagta gaagatcctc gacaggagct tgctgtcgaa gcgcaggtgc
     6721 atgaagaacg aggcgatcag gatgaacttg accaccatca tgaccaccag cagcacgacc
     6781 gccgggtagt gcgatccgtc gccgaaccac gacgaccaga agtaggtgga cacctcgagc
     6841 gccgtgatgg cgacgagcac ccagaagatc gtgaagtagg tggaggtcga cggaccgtgg
     6901 gtgtggtgtt cgcccggatg caccggggag tcgtcgtcga gttcctcaac ggtggtgctc
     6961 acgctggctc ctgcgtcgtc tcgggaaggg gtcgttcggg gtcggtgcgg tggcgggtca
     7021 cgggatcagg tagacgatcg cgaagatcac cacccacacg atgtccacga agtgccagta
     7081 gaggcccagg acctcgacgg cctcggcctg cagctgcttg cggaacgagt agccgacggc
     7141 gctgaacagc atgatgatgc cgatcgccac gtgggcgccg tggaagccgg tcagcgtgta
     7201 gaacgccgag gccgacacgt tggaggtgta gccgagcccc tcgcggacga actcggtgaa
     7261 ctcgtagacc tggccggcca ggaacagccc gccgagcacg caggtggccg acagccacag
     7321 acgcatccgc ttgtggtccc cgcccatgac ggcgttgacc gccaggacca tcgtgagcga
     7381 gctcatcagc aggatgaagc tcgtcatcga cgtgaacggg atgttgaaca gctccgacgc
     7441 cttgagcggc gacccggcca ggcccgggat gctgccgggc cggttcttgt agagcaggta
     7501 ggtggagatc agaccgccga acagcaggca gtccgaggcc aggtacaccc acatcgccat
     7561 cttcgtgttg gagatgccgg tggaggtggc gtgggtgtcg tcggggaact ccaggtcctc
     7621 gaccggttcg agggcgatgt cagccaacgg tcgtctcctc cgtgctggcc attgccggct
     7681 cgagcgtgcg ctcgtccgcg tcgtcggagc tgccgtccac ctcgccgtca ccggcctcgg
     7741 cgtcggtcgg ggcgtcctcg gagccatcga cggcgtcggg ccggtcgtcg gtcgggtaat
     7801 cgtcgtcgtg gtggtgcccg gcgctcgggt cggtcgacgg ctcgaacacc cagccgaaga
     7861 tgcccaggat gatcaggagc acacccggga ccagccacca gtagttgaag atcaggccgt
     7921 agcccacgaa cgggaaccca caggccatga ccagcggcca gtacgacggc gacgggaggt
     7981 gcacgtcggt ggcgtcaccc ttctgcacga cgtcctcggt gcgggcgatg cgaaccaggc
     8041 ggccgttctc gtcatggccg tacttgcggt gccagaactc gtcgacctcc tcgaccaccg
     8101 ggagctcgtc gaagttgtgc tcgggggccg gcgacgggat catccactcc ggcgaacggg
     8161 cgtcccacgg gtcgggcccc ggatcgacgg ggttggcctt gtgacgccgg tagctcagag
     8221 cgacgttgac catgaacagg atcagcgaca cggcgatggt gaaggcgccg atcgtggcga
     8281 tgaggttcca gaagttgaag ccctggccct cggtgtaggt gtaggtgcgt cgcatcatcc
     8341 cctgcagccc gaggatgtgc atgggaccga acgtcaggtt gaacccgacg agcatgatcc
     8401 agaagttcca cttgcccatc ttctcgttca ggaagtggcc gaacatcttg ggccaccaga
     8461 agtacatgcc gccgaacagc ccgagcagcg cgccaccgaa cagcacgtag tggaagtggg
     8521 cgacgatgta gtaggtgtcg gtctgctggg cgtcggcggg ggcgaaggcg tgggtcacgc
     8581 ccgacaggcc gccgatggtg aacatggcga ccaggccgat cgagaacagc atcggtgtgc
     8641 tcaaccggat gcggccgccg tacatggtgg ccatccagtt gaggatcttc acgccggtcg
     8701 gcacggcgat gaacatggtg gcgagcgaga acgccagcac cgacagggtg ccgatgcccg
     8761 aggcgaacat gtggtgcgcc cacacgcccc agcccatgaa gccgatggcg atgccggagc
     8821 cgaccatgaa cgggtagccg aagatcggct tgcgggagaa caccgggatg atctcggaca
     8881 cgatcccgaa gctgggcagg atcatgatgt acacctcggg gtgcccgaat atccagaaca
     8941 ggttctgcca caggaacggg tcaccgccct tcgaggcgtc gaagaaggtg gcgtcgaaga
     9001 gacggtcgaa catcagcagg aacagagcca cggtgatcac cggaagggcg aacaccagca
     9061 gcacctgcgt gacgaacgcc atccaggtga acaccggcat gcgcatgagg gtcatgcccg
     9121 gtgaacgcat gttgagcacg gtgacggtca ggttgatggc cgacacggtc gagccgatgc
     9181 ccatgatgat gaggccgacg gcgtagaagt cgatgccgtg gcccggcgag aaagtcacgc
     9241 ccgagttggg ggcgtagttg aaccagccgc cgttggcgcc gccgccgagg aaccacgagg
     9301 tgttgaagaa gatggcgccg ctgagccaga tccacagcga cagggcgttc atccggggga
     9361 aggcgacgtc acgggcgccg atctgcaacg gcagcaggta gttggcgaac gccgactgca
     9421 tcggcatgac ggcgaggaag atcatcgtca cgccgtgcat ggtgaagacc tggttgtaca
     9481 cgtcggccga cagcagcttg gcgcccggga ccgcgagctg ggtgcggatc agcagcgcct
     9541 cggacccgcc gaccagcagg aagaagaagg cggcgacgcc gtacatgatg ccgatcttct
     9601 tgtggtcgac cgtgaagagc cacgacttcc agccgtgggt cgccgtgggg cgggcgaaca
     9661 cgccgagggg cttggacacc gtggcggcgc cgctgccgag ctcgagcggg ggatgatctt
     9721 cgacgagtgc catgagcagt ccttctgatc cttctccggc cggcctactt gagggtcacc
     9781 aggtaggcga cgagctgatc gatctgggtc tccgagagct gcaggttggg catgccctgg
     9841 ttgttctcgg ggttcatcgg cttgacggac tccggatcgc ggagccagtc cttgaggttg
     9901 gagcgatccg ggacgccgtc ggggacggcg gtgaccgact cggcggcctt gccctggtac
     9961 agcggcagca gcgagccggc gaacgtctga cgcatcatca ggtgtgtcag gttcggggcg
    10021 ttcttggacg tgagcgtgct gtcgaccgtc tggccgtact cggggtcggg caacttggag
    10081 tacttgaatg gcggcgatga ctccggtgtc agcccgtcga cctggtggca gcgggcgcac
    10141 tgcgagacga acagctcctg gccggcgatc gccttggggt cggtcggctg gtccgggacc
    10201 gatgtcatgg cgtcgaccca cttgtcgaac tcggccggcg gcagggcctt gagctccatg
    10261 cgcatcacgc cgtgggacag gccgcagtac tcggtgcact ggccctggta gatgccgggc
    10321 ttgtcggcgg tcatgatcag cgtgtgggtc cgtccgggaa ccgcgtcgag cttgccgttg
    10381 atggccggga tccagaacga gtggatgacg tcgttggact ggagcttcat cttgacgtcg
    10441 cgtccgaccg gcaccacgac ctgggtggcg gtgatgatgt cgaccgtgcc gtcgttgttg
    10501 gtgtcgtagc ggtactccca ccaccactgc tggccgatga cgcggacctc gatcgggttc
    10561 tcggacttct cgatctcgag cacggtcttg acgttgaaga cggcaaggat ggccagcacg
    10621 agcgccggga ccagggtcca gccgatctcc agcgtggtgt tgccgtgggt ctgggtgggc
    10681 tcgccgacgc cgtcgacgtc gtccttgttg cgcttgaagc gcacggccat ccagatgacc
    10741 ccggcctcca ccgcgaggaa caccaggcca gcgatgaaga acaccggctt gaccaggttg
    10801 aagatctccc agttctgctt gccggccggc gccagggtgt tgagcggcat ggccgggtcg
    10861 ccgatgttgc ggacgatcag ccagaggcag atcgtcatga cgaccagcga gacgatcccg
    10921 gcgatcgccg cgttgcgctt gttccgcatt cccaacctct cactcaccgg cgagcagggt
    10981 gagagtatcc accgccccgc tcccggaagc cgaatcgacc acgacggtgg gggcgatacc
    11041 cgcaccgtgc tcctcgcccg tctgctcgcc ggcgacgccg ccggcgatcc ccgcggcgat
    11101 gatgaccacg ccgccgacca gcagcacgga gaccaccacc gtcctcgtca gctcgggtcg
    11161 gctggcgagg atgaaggcca tcacgaagac gatgatcgcc gccatgatga tcatgtagac
    11221 ggcgacctcc tgcgggatcg ccagcaggag gcgggagatg cacaggacga tgacggccag
    11281 ggtcagcacc gacaggaccg gcacctcgag cgggtccatc aggtcgtggc gcagctcacg
    11341 gttggcgccc tggtcgccgg tggcccgctc ggcccacgcc cggacggtcc aggagaccat
    11401 gacgatggcg agcagcacga cgcccaccca gaacagggtc gtgtcgaccg ccaggccgac
    11461 cacggacacc gcgacgccga aggcgccggc catcggccag tagctgagcc cgaccggcgt
    11521 gagcaccggc gggttggtgg cggtggccgg gtcgcggccg tccatggcga ccagcacgtc
    11581 cgcgctgccg tcgcggaagg cggtggagaa gcctccgagc gccgccatca ccgcggcgaa
    11641 ccccatcagg acgctgtagc cgacgtggtc gccgacccag cccttccacc cgaaggtgag
    11701 ggggccgacg atcgagttca cgacgtcgcc gtcctggaac acgccgatca cgccgctgtg
    11761 cgcggcggcg ccggtcagga agccgtagac gatcgccgtc acgaagccga cgaccgctgc
    11821 ggcgtagaag aacttggaac cccgggtgat catcgagctc tcacttcatg atgtagacgg
    11881 cgacccagac caccgcgtag accgcgcagc tggcgtacca gaagactgcg gctgcggcga
    11941 tgccgtcggg ctgacgactg gagaactgac cgccgagggc ccggaaggcc atcagggcga
    12001 tgaagatcat cccggcgacc accatgagca ggctgcccac ggtgacggcg tagaagagcg
    12061 ggccctcggg ctgctcgatg gtgaccgccg cctgcttgaa caggaagatc gactggttca
    12121 cgaacgcggc gcccagcagg agggtgatcc ccagcgccag gtacgtgtgg gtccggtcgt
    12181 cgcgggcgat cgcgtacacc gcccactgca tggtcaccac cgacatggcc agggtcaggg
    12241 cctgcatgtt cggctgggtc aacgggatgg tgttggcgct gaaccagtcg gcgcccccgg
    12301 cgttgcgggc agcgaggtag tagccgatca gcgtggccat cacggccacg acgccggcag
    12361 tggcgaacgc ggtgccgaag atcagctccc gacggcgggg ctgcaacggg gcgggaaggg
    12421 cggccgtggc catcagggct tgtcctccgt gccctcacgg gcgtcgagca acgggtacgg
    12481 cgatgtcacc cgggccagct cgggcaggcc gatgcctccg ccggcggggt tcgggaacga
    12541 ccactcgagg gtgagaccct gcaccggctc gccggccggg tcctcggtgg cccgcatggc
    12601 ggccaccagg gcgctcagac cgccgagggc cagcaggccg gccccgacgg cgacgaggag
    12661 gccgaagatc tggaaggcgg tcgtctcgcc gtcgagctgg accaccgact gcaccagcag
    12721 ggtggtgccg aggaggccgc cgccgagaac gagcagcggg acggtgagcc gcccctcgac
    12781 cgagttgccg ggcccgcccc acagcagcgg cgaccagtgc aggctggcgg cggcgccgcc
    12841 gaggagagct gctccggcga cgaaggcggt ctgggccgac gccagggtgg ccggcgaggc
    12901 gccgaacagg gtgccctggc cggcgagcga cagcgactgg acccagccgg tgaccgcggc
    12961 ccccatcagc agcaggaccg agacgatcga gaggatcagc gccgggctca cgacgggctt
    13021 gccctggcgg agggagtcgc cgatcgcacc gagcagcccg aggatcggga cggtgatgcc
    13081 gaaggcgaac agggtccaca gcgcggtgtt gagcgcgctg ggcagctgcg cccacgcacc
    13141 gaacgccagg atcgcgtaga gcccgacgat gagctggagg acgtcggtgt gggcgaagcg
    13201 gcgcccgctg gcggtgacga ccacgtcgat ggcgatgccg agcaccggga tggcgaagac
    13261 gaacaccgcc ggtccccgca ggagccagcc cagcccgtcg gtgaagttgg tggcgagggc
    13321 ggccgggccc atgcggccga cctggccgag cacggtgtgg gcgatggccg aacccagcgt
    13381 gaccagcagc agcgcggcgc tgaccagcga tgcccacgac agcccgggaa cccatgccag
    13441 gcgcatcccc aacggccggt gcgccatgac cgtggtggcc acgcacaccg ctccgaggct
    13501 cagcgcgacc aggaccgctc cgagcgacac gttgccgagc ttggccgcgg tctcgtcgcc
    13561 gcccaggact ccgccgtcga gggcgatgct caccgcgaag atccccgacg acagcagcca
    13621 ggtccagaac gacagcgtgg cggcccgggg gaaggcgatc gacggcgagc cgagctgcag
    13681 gggcaccacg gcgatggcga cgccgagcag cagcggcacg atgcccagga acagcagggc
    13741 gacctgggcg ctggcggcca gctgggccgg acgcgaaccc aggaagccgt tgtcggtgag
    13801 agcgtcgacg ccggtcaacg cccacagagt gagcgacagg ctggcgaggg cgaacccgaa
    13861 gccgatgaac aagcggccga tcgaggtgtg gtcaccggag ccgatcagct gctccactgc
    13921 gctcggggcg cgccgggcgc gatcgcccgc ggacgtcgcg tccgtgtccg acctggtctc
    13981 ggtgagtgcc atgcgtggtt ctgccctctg cctcgtctga ctgctgcgac cggaccggtt
    14041 cgcctgtccg gatccctgcc cggtcctgtc cgcgcggaca ctaccgcgac cgggcaggta
    14101 ctgagaaaag cgtcaggacc ggtctaccag ccgttccgga tgatcacgtc gagcgccatc
    14161 accccgaaca gcacggtgat gtaggtgatc gagaacgtga acacgcggat ggcacgggca
    14221 ggcgactcgg tccgacgcag atccacagcc atccaggtga acaccgcacc gagcacgatc
    14281 gccgtcgccc agtagaggag ccccatctcg gcggtcacgc cgaagagcag cgtcagggcc
    14341 cacacgacga cggtgtaggc gaggatctgg tcggtggtct gacggtggct ggccaccgag
    14401 ggcagcatcg gcacgtcggc ccgggcgtag tcgtccttgt acttgatggc cagggcccag
    14461 aagtgcggcg gggtccagaa gaagatcacc ccgaacagca cgatcgccga gatcgacacc
    14521 gtgtcggtga ccgccgacca gcccaccagc accggaaccg cccccgcagc gccgccgatc
    14581 acgatgttct ggcgggagct gcgcttgagc cacagcgtgt agatgaacac gtagtacagg
    14641 cagccgctga cggcgagcac cgcggagagc aggttgacca cctgccacag gaacacgaac
    14701 gccaccacct cgatggtgac ggcgaagacc aaggcgttgc ggggggtgac cgccccggtg
    14761 accagggggc gaccctgggt gcgctccatc accgagtcga tgtcgcggtc gacgtacatg
    14821 ttgatggcgt tggcaccgcc ggcggcgagg gtgccgccga tgacggccgc ggccatcagc
    14881 cacaacgggg ggacgccctg gtcggccacc accatcaccg ggaccgtcgt cacgagcagg
    14941 agctcgatga tccggggctt ggtgagcgcc acgtaggcgc cgaccgtggt cgccagcttc
    15001 gctccgatcg aggtcgggga ggagatcgcc ggggcggcca tgacgtccca acgctacggc
    15061 gccggcgccg ccgatggcca gcccggcagc gactcccgcc cgccgcggcc cgcgacctac
    15121 cctggccgtc cgtgaccctc gaaccccgca cgtaccggcg catcgtggcg atcgcgctcg
    15181 cgctgctgtg cgccatcgtg gtcaccggag ccgcggtccg cctgtcgggt tccgggctgg
    15241 gctgcgacga ctggccgaac tgctcgtcgg agagggtgat cgagttcggc aaccccaacc
    15301 agaccatcga gcaggtcaac cggctcttca ccggtctggt gtcgctgtcg gtcgccgccg
    15361 cggttctggg ttcgatccgc cgccggccgt accgccggga cctggtggtg ctgtcatggg
    15421 gcctggtcgc cggggtgatc ggccagatcg tgctcggcgg gatcacggtg ctggtcggcc
    15481 tgcaccccgc cgcggtcgcc ggccacttcc tgctgtcgat gatcctggtc gccgacgcgg
    15541 tcgtgctcct gtggcgctcc ggccaacccg acggaccccg gcggccacag gtcgcccccg
    15601 ccgagctgtg gctgtcgagg gtggcggtgg tcctgatggt cggcctgatg ttcaccggcc
    15661 ccgcggtcac cgggacgggc ccgcacgcag gtgacgccga gtcgccgcgg ttcggttggt
    15721 tcctgcccga cgtggcccgc atccacagca tcaacatgtg gatcttcctg gcggtcgtcg
    15781 tggtgctgct ggtccgcctg gcccgctccg gggccccacg tccggtcctg caccgcgctc
    15841 aggcgctgct ggtcgtcatc gtcgcccagg gagccatcgg atacacccag tacgagatgg
    15901 gcatcccgcc ctggctcgtc gccctgcaca tcgccggggc gacggccgtt ctgggcgtca
    15961 ccgtgtggtt ccacctgggc ctgtcggcac cggtgaccgc tccggacggc atcgacggcg
    16021 acggcctcga cccggtccgg accgacgaca aggacggcac ggtcgatcgc gccggctcct
    16081 cgtcgtcggc gtagccttcc ggcgtgagca ccccggcccc gatcgacgtc gtagagcccg
    16141 acgtctccga cctggtggac ctcgacaccc cctgggtcgt cctggtgtgg aacgacccca
    16201 tcaacctgat gagctacgtg accttcgtgc tgcagaagct gttcggctac tccaaggagg
    16261 aggcgaccac gctcatgctg caggtccacc acgacggcaa ggcggtggtg tcgaccgggt
    16321 cccgcaccga ggccgagcgc gacgtgttcc gcctccacga gcacggcctg tgggccacga
    16381 tgcagcagga ctgacacccc gtggcccgat tccgccccgc cgtgaccgcc gacgacggcg
    16441 acacctacac gatctcgctc gggcccaacg agcgcgacct gatccgctcg gttaccgagc
    16501 agctcaagga gctcctggtc gagaccgacg ggccggtcct gcgtcggctg ttcccacccc
    16561 cctacggcga cgacgacgag cgcaacgagg gctacgcggc gctggcccgc cccgagctga
    16621 tcgagaaccg gctcgcatcg ctcgacgtgg tcatcggctc cgcggaggcc acgacgctcg
    16681 acgaggactc gatggcggcg tggatgcgct cgatcaacga cgtgcgcctg gtgctcggga
    16741 ccgtgctcga catcaccgac gactccgaag aggtcgaggt cgacgacagc aacgtcgcga
    16801 cgtaccaggc gtacgaggtg ctcgggatgc tcctcgagac catcgtggcg gcccggtcct
    16861 cgcggctctg agcgatccca ccccgtcgga ccgatgcccg gttggtgctc cccggcggtc
    16921 ctgctatgtt gtcccggccc ctccacgggg atgggagccc ccatcgtcca gtggcctagg
    16981 acgcctccct ttcaaggagg taacacgggt tcgaatcccg ttgggggtgc agcaaggcaa
    17041 agttcgtggt cccgtggtgc agtttggagt gcacgccggc ctgtcaagcc ggaggtcgcg
    17101 ggttcaaatc ccgtcgggac cgccacttcg cccgttccgg atcgatccgg ggcggtcgga
    17161 gcacggtcgg gtagctcagt tggcagagcg accgcctgaa aagcggtagg tcaccggatc
    17221 gacgccggtc ccgaccacac tccggaaccc ccgcccgtgc gggggttccg tcgcgtccgg
    17281 acccgacgac gaccaccgaa cttgtagccc acaggtcacc acgcgaccca ccagctacaa
    17341 gttcggggat gtccaccgaa ctcgtagccc acgggccacc acacgaccca ccagctacaa
    17401 gttcgggggt gtcagcggca gacgccgtcg gcgcgggccg cctcggcgca cgcctcggcc
    17461 accgccaccg acacccgcgg gttgaacacc gacgggatga tgaagtcggg ggccagctcg
    17521 tcgggttcga ccgtcgaggc gatggcccgg gccgcggcga ccttcatgtt ctcggtgatc
    17581 cgacgggctc ccgcgtcgag ggcgccgcgg aacacgccgg ggaaggcgag cacgttgttg
    17641 atctggttgg ggaagtcgga acgcccggtg gcgatgaccg cggcgacgtc ctggatcaac
    17701 tcgggtcgga tctcggggtc ggggttggcc atggcgaaca cgatcgggtt cgacgccatc
    17761 cgctcgacgt cggagcgctc gatcaggttg gggcccgaca gcccgatgaa cacgtccgcg
    17821 cccggcatga tctgggtcag cgatcccgac agcccctcgg ggttcgtgtt ctcggcgaac
    17881 cactgcttgg agcggttcaa cccctcccgc gacgtgtgca ccgcgccctg gcggtcggca
    17941 ccgatcacgt tgcggacccc ggcgttcatc aggatcttcg acacggcgac gccggccgcg
    18001 ccgacgccgg ccacgacgac cttgaggtcg gccatgtcct tgtcgacgat cttcagcgcg
    18061 ttctccagag cggcgagcgt gacgatggcg gtgccgtgct ggtcgtcgtg gaacaccggg
    18121 atgtccatgc actcgtcgag gcgctcctcg acctcgaacg cgcccggggc ggcgatgtcc
    18181 tccaggttga ttccgccgaa ggtcggctcc aaccggagca ccgtctcgat gatctcctcg
    18241 gggtccttgg tgttcaggca gatcgggaac gcgtcgacgc cggcgaagtg cttgaacagc
    18301 agcgccttgc cctccatgac gggcatcgcg ccctcggggc cgatgtcgcc gaggccgagc
    18361 acggcggtcc cgtcggacac gatggcgacg gtgttcttct tgatcgtgta gcggtgtgca
    18421 gccggcgggt tcttggcgat ctccatgcag acccgcgcga cccccggcgt gtaggccatc
    18481 gacaggtcct cgacgtcgcg cacgggcgcc agcgagagga cctcgatctt gccgccctcg
    18541 tgcatgtgga aggtgcggtc ctcgaactcg aggatctgga tgccgtcgat gccctcgacc
    18601 gcggcgcgca cctgctcctg gtgggcttcg ctggcgcagt tgacgacgac gtcctcgtcc
    18661 aggctggcgg tcttgacctc gaatccctgc agcgccgaga tgttggcccc taccgccccg
    18721 atcgcgatcg ccagctcgcc cagcatgccg gggcggttct ccatgcggac acggatgcga
    18781 acggaatagg cgggagtggg ggtgtcggcc atacgtcccc cgacgctacc gccggaccgg
    18841 ttcggggacc gaaagggtct ggcggcgttc agggaccctc gtaggcgaac cccaggtccg
    18901 gcgcggtcac gagcggtcgg taggcgatcc ccgcctcggc ggccatcgcc gcgcaggtcc
    18961 cgccccggtc gaccagcggc atgatcagca ccggctccgc gccgagctcc cgcacgacct
    19021 cgaccgcttc gaacagcgac gtcccacggg tgacggtgtc ctcggtgatg gcgacccggt
    19081 ccccggcgcg cagcggcccg gcgagtcggc cggtgacgcc gtggtccttg gcctccttgc
    19141 ggatggtgaa gctccgcagc cgccggcccc gggtggcggc gacggccgcc accccgtagg
    19201 cgaccggatc cgcgccgacg gtgagcccgc cgatggcggt ggcgtcgtcg gggatgaccg
    19261 cgagggcggc gccggccatc agcaggacgc cgtcggggtc gcacgcggtc tgcttggagt
    19321 cgatgaacca ggagctgcgc cgaccggact tgagggtgaa gtcgccggtg cgcaccgagt
    19381 gctcgagcag gtgggcgacg agggcgcggt ggacgtcggc ggggatgccg gggagggtca
    19441 tggcagcgac gctaccgccg cccggtcagg tcggtcgggg gtcccagccg cgcaggccgt
    19501 agcgcgggta gtcaccgatg aagaagaact cgtgcaggcc ggaaccgacc gctccgtcgg
    19561 cgacaccacc ggtctgggtg aagcgggcga cctggtcgac cagaccaaac aaccggtccg
    19621 cgtcgcggcg gtagtcgatc tcgaccccct gcacgaccag cggcccctgg tacatgccgt
    19681 gacgccagtc ggcctcgatg ccgtagccgg tgccgagcat cagccagcag tccatgagcg
    19741 gctcgaccgc gacgtcgatc gcgccgccgg gcgcatcggg gaacgacaag gtcgaggact
    19801 cgatgcggcg ggtgccgggc gtgatcaccg cgtcgtgctc gggtcgaccc aaccactcgg
    19861 gctcacggtc ggggtcgttc cagatgcgca ccgcctcctc gtgctcgcgg tggccgtcgt
    19921 cgtgctcgtt gaggatgtag aggatcgagt ggtcctcgaa ctgcatcggg gcgtagttcc
    19981 acatgcccgt catctgccct tcggtgcgga tgcccgccgg ctcctcctcg ccgacgggtc
    20041 ggatgcccca gctgcggtca cgggtgcccc accagcggtc gggggtcacg tcgaaggtgg
    20101 tgccgccgct gcggatcgtg ccggaccacc gaccggtctg ggcgagccgc atcgagtcga
    20161 acagggtgcg gccgtgacgg cggacgtact ggcggggctc ctcgaaggcg gggatggagc
    20221 ccacccaccg caggtcgacc gacacgtcgc tgtgctcgtt gggttcgcac acgatgcgca
    20281 gctcgtgcag cggccggatc acctcgacgc gcaacggccc caccgaggtg tccatccggt
    20341 cgccgagctc gcgcgacgcc cgcaccaccc ggtggtcgtc cccggagacc acgcacacga
    20401 aggcgtcctg caccgccagg ttggggtact ggcccagacc gaagatcagg aacgcggagc
    20461 cgtcgtccgc gtggcagttg aagtagtagc ggtcgtagaa gttccggtcg ctggtggtgg
    20521 cgtgacggac ggtctcggcg gcctggtgga cggggtagtc gtcccacgac gacagcgatc
    20581 cgtgggcccg gggagcgacg aagtcggtca tggcgccgac ggtacccggt gcgtcgccgc
    20641 cgacgttcgg ctgcgggacc gacgcgtccg cccgcgttcg gtgccggtcg cgccgtcggg
    20701 ggtcgcgctg tcgcgggtct gtgcctcagc tggtgaagcg cacccgaccg gagccggcga
    20761 cgacctcgcc gatgaccgcg gcggccagcc cgtgcccgtc gagcacggac gccgcgtcgg
    20821 ccacgacctc gggcggcacg gccacgacca tgccgatccc caggttgaac acgcggcgca
    20881 tctcgtcggt gtcgacgtcg ccgatccgct gcagctccga gaagatccgc ggcgcctccc
    20941 acgaggtcgg atcgaccacg gcgtccgcgg cgtcgccgat cacgcggttg aggttgccgg
    21001 gcaggccacc gccggtgatg tgggccacgg agtggaccgg caccgcgtcg atgagcgccc
    21061 gcaccgccgg ggcgtagatg accgacgggc gcagcagctc gtcggcgacc gtcggtgcgt
    21121 cggcgtcgtc ccaggccgga tcgtcgagac cccggccggc cacgtcgaac atgaggcgtc
    21181 gggccagggt gtagccgttg gagcgcagac cgggcgacgg cagagcgacg agcacgtcgc
    21241 cgggggtgac cgactcaccg gtgatcatgg ccgagcgctc gaccgctccg accgcgaagc
    21301 cgaccaggtc gaactcgccc ggctccatca cgccggcgtg ctcggccatc tcgccgccga
    21361 ccagagcgca gccggcctga cggcaaccct cggcgacgcc ggcgacgagc gactcgatga
    21421 cgtcggggtc gaccttgccg atcgagatgt agtcgaggaa gaacagcggc tcggcgccct
    21481 ggcagaccag gtcgtcgacg cacatggcga cgaggtcgag cccgatcgtg tcgtagcggc
    21541 cggtcgcggt ggcgatcagg gccttggtgc cgacgccgtc ggtggtcgac accagcaccg
    21601 gctcgcggta gcccgcggcg tcgaaggcga agaggccccc gaacccgccg atgccgccga
    21661 gcacctcggg tcggcgggtc gagcccacgt gggagcggat gcggtccacc gcgtcctcac
    21721 cggcgtcgat cgacaccccc gctccggcgt aggtctcacc catcggtgac cgtcccgtcg
    21781 gccgggccgg acccggtctc ggcggcgagc gacccggcgt cgaccgaggg cagcactccg
    21841 gcatccgcga ggagcaccga tcccatctcg gacaaccctt cgcgggcgac cggtgcggcg
    21901 ccgtcgagca cgcccttgcc gatctcgacg gggatctcga ccgggtagac gccggtcagg
    21961 cacgcgtcgc agaagccggc gcccggagcg ccgatggcgt cgatcaaccg gtcgagggtc
    22021 aggtaggcga gggtgtcgac gttgaggtag cggcggatct cgtcgacctc gaggttggcg
    22081 gccagcagct cgccccgggt gccggtgtcc atgccgaaga agcacggcca gcggtacggc
    22141 ggcgacgaca cgcgcaggtg cacctctcgg gcgcccgcct cgcgcagcat cgccaccatc
    22201 gccttggtgg tcgtgccccg cacgatcgag tcgtcgacga cgaccagccg tcgcccctcg
    22261 atgttgtcgc gcagcgggtt gagcttcatg cggacaccca gggcacgcat ctcctggctg
    22321 ggggcgatga acgtgcggcc gatgtagcgg ttcttgacca gcccctggcc gaacgggatg
    22381 ccggagcggc gggcgtagcc ctcggccgcg gggatgccgg actcggggac gcccatgacc
    22441 atgtcggcgt cgaccggggc ctgatccgcg agctgctccc ccatgcgcac ccgggcctgg
    22501 tgcacgttgc ggtcgtagag ctgagagtcg gggcgggcga agtagacgaa ctcgaacagg
    22561 cagagcttgg ggtcgatgct cgagtcatcg aacggtcgca ccgaccgcac cccggcggcg
    22621 tcgatgacga ccatctcgcc cgggtcgagc tcgcgcacga agtgggcgcc gaccacgtcg
    22681 agggccgggg tctccgacgc cagcacccac ccgctgtcca gcttgcccag gcacagcggc
    22741 cggaaccccc gggggtcacg gacgccgatc acccggttgc ggtccatcag caccaacgag
    22801 aacgcgcctt cgaagctggg caggacggtc accagcgccc gttcgagggt gtcgtcgccg
    22861 gggccgagcc gttcgcactc gagggcgatc acctcggcca tggcgtcggt gtcggacgag
    22921 acggtgcccg gcagcatgcc ggcgtcgtcg accagctgcg cggtgttgac caggttgccg
    22981 ttgtgggcca gcgcgaactg ctggtgcacc acgtcgcgga acaccggctg ggagttcttc
    23041 cacatgctcg acccggtggt cgagtaacgg gtgtggccga tggcgaggtc tccgtcgagg
    23101 accgccagcg tgcggtcgtc gaacacgttg gagaccaacc ccatctcctt gaccacggtc
    23161 aggtggtcac cgttggagct ggcgatgccc gcggattcct ggccgcggtg ctgcagggcg
    23221 tagatcccca ggtaggtcag gtgggcgacc ggggcgccgg gggcgtacac gccgaagacc
    23281 ccgcacgcct cgcggggctt gccgccctcg ggatcgacct ccaggtccgg gcccccggcg
    23341 acgccgtcac cggggggacg ggagtctggt tcaggggcgt gcggaccgtc gtcgttcgag
    23401 gcggggagca cggtcccatg atcgcacatc gacccggggt cggacacagc cggcctcacc
    23461 cctccatcgc cacgtcgtca cgccctcgcc gcccggccgt cccggaacac ccagggatca
    23521 cgaaccagcc gcgggagcgc gaaccggtcg gccacggtgg cggcgtccag acgctccccc
    23581 ggctcggcca ggccggcgga tccggccagc atcccgacga cctccgcgac gtcctcgccg
    23641 agctccgtgc ggtccgccag ggtgaccgcc ccgtcacgtt tggacaggcg ccggccgtcg
    23701 gcggcgagca cgagcgggac gtgcaggtac tccggggtgg ggaggtcgag cagctgctgc
    23761 aggaggacct gtcgaggtgt ggtctcccac aggtcgtcgc cccggaccac ctgggtgacg
    23821 ccctggtcgg cgtcgtcgac gacgacggcc aggttgtacg cgacctcgcc gtcggcccgt
    23881 cgcaggacca ggtcgtcgac cacgacggag agcgtcccga ccatctcgtc ggtgacggtc
    23941 acccgtgccc cgtcggcccg gaggcgcagc gccgggcggc ggccatcgcg ttccagggcg
    24001 tggcgggccg tagcgctgag gtgccgacag gtcgccgggt acgcgccgtc ggggtcaggt
    24061 ccgtggggag cgaccgccgc gtcccggatc tcccggcggg tgcagtagca cgggtaggtg
    24121 agcccggcgt cgaccagtcg ggcgatggcc gcgtcgtaga ggtggcgccg ttcggactgc
    24181 cggacgacct caccgtcgtg ggtgatgccg acggccgcga ggtcggcgag ctggtcggcc
    24241 gcgaccgcgg cggtccgctc atcgccggcc gaggtggtca ggtcctcgat gcggatcagg
    24301 aaccgtccgc cacgcgacct cgccgccgcc caggcgagca gcgcggtgcg caggttgccc
    24361 aggtgcagcg gggccgacgg gctgggagcg aagcgacccg tcaacac
//
LOCUS       DAOQOD010000024        24321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_697_length_24321_cov_12.062969, whole genome shotgun sequence.
ACCESSION   DAOQOD010000024 DAOQOD010000000
VERSION     DAOQOD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 24321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24321
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_24321_cov_12.062969"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLZ72_04225"
     CDS             <1..1910
                     /locus_tag="PLZ72_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNA35539.1"
                     /translation="PARYIGCEDGAATPDWSAAGPTSPVVAWLMTYPDTYEIGLPNQG
                     LQILYEILNERPDALAERSYAPWIDLEAEMRATGVPLFSVDTHRDAGTFDLLAFNLSA
                     ELTYTNLLNCIDLAGVPVRAADRRAHHPLVVIGGHCAYNPEPVAAFVDLVVLGDGEET
                     VGDITRIVSDWKASGRGEREAVLRALCAVPGVYVPAGYEAHYDGQDLVAVEPIWPEAP
                     EVVEKRSIADLADWPYPRSQLVPLTEVVHDRLNVEVFRGCTRGCRFCQAGMVTRPVRE
                     RPAEQVRSMVAGGLRRTGYDEVALTSLSTADFSGIGDVVGGIIDDEACGRVSVSLPSL
                     RVDAFGVDIASQLQRGRRTGLTFAPEAGTWRMRQVINKLIREQDLYDAVESAYSQGWR
                     RMKLYFLTGLPTETDEDTLGIAELAANCVEIGKRHHKGPSVTVSLGGFVPKAHTPFQW
                     FGQNTQVELQRKVHLLRDAVRRHHGVQMKWHDPRATLAEGIASRGDRRLAPVIEEVWR
                     RGGTFQEWSECFRIDLWEEAMAAHGLDIEWYVYRHRREDEVLPWDHISAGLHKDFLWQ
                     EWTDALAEVGLPDCRWTPCYDCGACTGYGIEHVVASATPPAGGSQGTGQDLSTGGAVP
                     VSLSPRPTVSA"
     gene            1910..2635
                     /locus_tag="PLZ72_04230"
     CDS             1910..2635
                     /locus_tag="PLZ72_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNA35540.1"
                     /translation="MEVRVRYAKIGKVRFLSHRDLARILERAVRRAGLPVAYSQGFSP
                     RARLHFGLALSTGYESLAEYLDIDLVEPDEGGTASALPAAEEVADLLQPCLPPGVVTT
                     ASTSIEAGTAALQDAVTSCTWEFLLDPADHDRATANAARMLAAEELPIDLVRKSKQVR
                     EDLRPLLLSLDVDRTPECSRLTVELGTKPRSVRPAELLAAMELPEHPIRVVRTHQWIQ
                     SDGERREPIELAAPSPHASERAS"
     gene            2648..4498
                     /locus_tag="PLZ72_04235"
     CDS             2648..4498
                     /locus_tag="PLZ72_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNA35541.1"
                     /translation="MSDTESNPPRPTGGTTEPAASGASAAPARPAAEAAPRPKIGDSR
                     PAPPVPAGGSGAGGPSPTGGSSGGSSGGQPGTGQSRSSRRRRRRRGGGGGGGQSGQGG
                     QNASGSQNQSGSGKSGQGKGGQGKGGQNRSKGGQGGQNRSKGGQSKGGQGRSGEGRSA
                     PTPVEAVLSDDTPVELDDETLERRRGRQRKGRAIGRYQMNVHVGSDATHIAILEGRSL
                     VEHFVSRPSDDVSQIHGNIYVGRVENVLPGMEAAFIDIATPKNAVLYRSDVQYDPDDI
                     ESKGGDQPRIEDVLKARQLIVCQVTKNPIAHKGARLTQEVSLPGRFVVLVPNSRTFGI
                     SKRLPDNERKRLRQILDKVKPERHGLIVRTAAENITADEIERDVARLARQWEQIEALA
                     AEQQRPGLIYREPEMAVRIIREEFNSEYRSVLIDDRALFDDMHDYVAAVAPALVDRVQ
                     FFDPDTESLPMFERFHVTEQLVKALDRKVWLPSGGSLIIEHTEALTVIDVNTGRNVGS
                     SSLEETVFRNNLEAAEEIARQLRLRDIGGIIVIDFVDMEVKENRDKVVKAFRDALSRD
                     KTRTQVFNISELGLVEMTRKRIGEGLLESVADRCDHCDGRGLLLPDELVG"
     gene            4562..4870
                     /gene="rplU"
                     /locus_tag="PLZ72_04240"
     CDS             4562..4870
                     /gene="rplU"
                     /locus_tag="PLZ72_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNA35542.1"
                     /translation="MYAVVKTGGKQYRVEEGQRLSVERLGDAGDDVQLRPVMVVDGDT
                     VLATPDQLSGASVTARVVGEAKGPKINAFTYKNKANERKRWGHRQSYHTIEITGITAG
                     "
     gene            4901..5158
                     /gene="rpmA"
                     /locus_tag="PLZ72_04245"
     CDS             4901..5158
                     /gene="rpmA"
                     /locus_tag="PLZ72_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNA35543.1"
                     /translation="MSKTKGGGSTRNGRDSNAQRLGVKVYDGGVVTAGSIIVRQRGTR
                     IHPGENVGIGGDDTLFALADGTVKFGSRRNRKLVNVVPLAD"
     gene            complement(5234..6403)
                     /locus_tag="PLZ72_04250"
     CDS             complement(5234..6403)
                     /locus_tag="PLZ72_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA35544.1"
                     /translation="MTPERSTPTPPAVDPSRAEEWVSTMSGHRHRSSDSSAVRHAHIG
                     PTGVVGPVSPLGRTAREESEGLSLVAGATLASGAGDRARDEAPDDWRTCFERDRDRIL
                     HGTAFRRLAGKTQVFVFPDDHQRTRMTHALEVAQVAVAISRAIGLNVALTEAIALGHD
                     CGHGPGGHASEDAFAPYVEGGYDHAVWGADVVLEPLNLCAETLDGIRNHSWSRPAPAT
                     PEGEVVSWADRIAYVCHDFEDAVHVGITAAELLPPVVIDRCGTRHSQQLGSFIGGVVD
                     AVAATGSVGMTEPLAEALGEFRRFNYDHIYMRPASVEQSQAVIRVLRALVEHHADRPH
                     ALPRPAEQLHGVEPGSTEAFRQAVTYVGGMTDRFAFDQARTLLRWPDAELPVGIG"
     gene            complement(6456..8024)
                     /locus_tag="PLZ72_04255"
     CDS             complement(6456..8024)
                     /locus_tag="PLZ72_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA35545.1"
                     /translation="MATTFADTTTLWELVERRADATPDALLLIDESGRTLTCGEFRDR
                     ALRAAAGFHALGVTHGTRVTWELPNRFETIVASFALARLGAVQNPVLHFYRHKEVGYA
                     IRATEAEFVLVPGEWAGFDFAAMVDELTADSAAPPVVIDVFADLPDGDPATLAGVAAP
                     TDGDEVRWVYFTSGTTSDPKGVRHSDRTLITGANGLAVALDMSPDDVGSIAFPFSHIA
                     GPDYFGTMLLAGFPAVLFEAFNPVSAIPVLAANGVTMVGGGTAFYQMYLTEQRKDPAT
                     PIIPSLRKMSGGGAPMPPEIFDEVKREFGVKICHGYGMTECPMICEGSPHDTDEQLAH
                     TVGKPVEGLDLRIVTEDGSVAGPGEDGEVRLKGPMVFKGYTDAAATAEAFDDDGWFRT
                     GDLGHVRPDGHVVLTGRLKDVIIRKGENISAKEIEDVLYAHPKVGDVAVIGLPDRERG
                     ERVCAVVERVPEADPADDLTFDEMVATLTDAQLMKQKFPEQLVIVDALPRNETLRKVL
                     KYKLREEYAAVPWP"
     gene            complement(8127..10166)
                     /locus_tag="PLZ72_04260"
     CDS             complement(8127..10166)
                     /locus_tag="PLZ72_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35546.1"
                     /translation="MSPGLRLLPLLLRRSPRQSAGAIVLCAVGVAIATLVAGVALGAY
                     HGFGARQPKVVWRSPTPAAEAHVSPVALQRRSTDWFDGRRIDRVDIAALDTTDQAPAP
                     PGLRAAPAPGTACVSPALAELIASHPSDQLGDRFGQVVGRIGAAGVAHPDELVAVVGT
                     TPDQLGPATSSGSPNAVLGPDRPSDTVPVASFDTTGADRDLMMYRSFALVAVVLVVVP
                     AVALIGSSARLTAARREQRMASLRLAGATSGAVRLLAVAETTIGAALGAVIGIASAMA
                     VSGLLVDIAMAGGRWFPADIRLPVSSAAALFVATIAVCAAATAVSLRRVGSRPLGVAA
                     SAEPASARWPRVLGLLAAIALLCVTTVAATAQTFTGSNGQPKDMSVPMVAALATVIAS
                     LALVGPWVTSIVGRIMVRSARRTPLLLAGRRVLEDPRAAYRTVSAVVLAGLTAGFLAG
                     VMPTVQALERDGLPSGAATAVLDRPAADAVLAAAGEERPVDTQPGGEVSEPVDVVVLA
                     SGPRLERIRTATTAIRGGTPLVASDDDLFGDITFTADAMRGSFAVLASGLLLAFGASG
                     VAAAAAVVDQRRLIGRLVLCGTEVSTLQRARRLQTVLPLAVATIGSVVIGLGSSFLLM
                     VGFGLSAARLASPNVGVLGLIVVAAIAMGLASASFSRPMLIAAARSATEGSPVSV"
     gene            complement(10163..10954)
                     /locus_tag="PLZ72_04265"
     CDS             complement(10163..10954)
                     /locus_tag="PLZ72_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35547.1"
                     /translation="MPLTAPELDPMQSPPSPWSPPSRPGPVLVATGLTKRYRDATALA
                     GVSISLEAGTATAVVGPSGSGKSTLLHCLAGFTRPDAGDVTLDGLLISRLGQRTATKL
                     RRERFGFVFQSDQLLGELPAVENVALPLMLGGMSRRSATARAAEWFAPLGLAGLETRR
                     PGQMSGGQVQRVSIARALVVGPSVVFADEPTAALDRATSTTTMEVLTRACRHAGAALL
                     VVTHDLDVAASCDRTVRMRDGRVHGVVTNPSAASASSTATAGSVG"
     gene            11096..12352
                     /locus_tag="PLZ72_04270"
     CDS             11096..12352
                     /locus_tag="PLZ72_04270"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA35548.1"
                     /translation="MDQHGLPTRTRTAPVPSSIDRILDRAQQAAPWIDRAGDLLLVLC
                     CAGFSLLDLAILLTSGRSETWLGVGFGALGAVALGGVSLVAVAAVALRRQLGLIALVG
                     MSVLSLCATTASLLLGVSLPPSFAVLFALAVLVARALHVEPELPAAGAALLALLAVLG
                     EPARLQDRGTVEPMQVLCLLGLGGAVVVGLWLRFGDWRRASNEAAVRSDERLALAREL
                     HDLVGHHVTGMVVQAQAGQLVAESDPAAAVESFARIESAGADAMSAVRRMVGDLRDDA
                     PRAPGAGWDDVDRLIADAVGDGVPVRCSVEAAARRVPPELTSSVHRIVAESLTNVRRH
                     ARAVTTVDVSVRVEDGDLVVDVHDDGAPSVHPAGSSRPDTFGLVGMRERTESLGGRFF
                     AGAAPGGGWLVSARLPVAAGERVRRR"
     gene            12349..13041
                     /locus_tag="PLZ72_04275"
     CDS             12349..13041
                     /locus_tag="PLZ72_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35549.1"
                     /translation="MIRVLLADDQEMVRVGFRMILDSDAEIEVVGEVPDGADAVRLAR
                     ELGPDVVLMDVRMPGTDGLEATREIVEGAVTARTGDPVRVVVVTTFDLDEYVDAAITN
                     GAAGFLLKDSGPALLIEAVKAAVRGDVLISPSITVRLLSHFAGVGSTAAGPAGAIGSP
                     RSAQPVVALTEREEEVLCEVAKGLTNAEIADVLFISLGTVKTHLGSMQDKLGARNRVE
                     LAAWAWRTGRMS"
     gene            13084..13815
                     /locus_tag="PLZ72_04280"
     CDS             13084..13815
                     /locus_tag="PLZ72_04280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA35550.1"
                     /translation="MTPPIPHARRSRVRSAVAPWAAALTRRSYPVLHAVATAAVAVGR
                     GALHWASLGPGDAGRVGFAAFGERSFIRQPYSDIVGAPGIAIGADTLIGSGVNLAAVP
                     QDEWSPGDEPLLRIGSRVWVAPGLHVVAHRRVEIGDDVWIGPGVYITDADHDRSDPDV
                     PIGHRMAPARPVRIGAGSWLGTGVVVLSGVTIGERVTVGANSVVCDDLPDGSVAVGAP
                     ARVVREASALPSAADDDQRRAGGDQ"
     gene            complement(13759..20505)
                     /locus_tag="PLZ72_04285"
     CDS             complement(13759..20505)
                     /locus_tag="PLZ72_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNA35551.1"
                     /translation="MLTFDVPRFSASWLRFRRIAPKLLAAVAATLVIGSLLVHTGTRP
                     AAAAPPLPVVGLDLDTTPFIGEQVPIDLTFSNPTPADTGYGPFTDLFLPTNGADGTSG
                     GGPRDGVSFTSATYLGATVPSTVTVLACDGSDVHPLTGQPVTCPAGFTAGDTMTTLTL
                     PFGSFTPTQPAATLSVLTQLSNFADLGHTLPIVARGGFRFGADPLANPLTDPPIIGAN
                     VSSSVTPSLITITKQDNGPESETATGPNFGRLWNVAVDLAPGQTVTNLRVTDALPDDI
                     AYSGISSITPSGTVTDQPAGPGPWAAESVTVTYPSATTHAAYDVGFWVPELDAAGNPV
                     IDPTGGAATTVSNDASAVGNWTPLDPRDPAGTDNAVADPAGPLDTITARSVATQKSVV
                     VATDTGPAGPTPGDTLTWTIQVQVSDYFDFEDLALDDIAADGQTLDVGFSPTISYRTP
                     DGSGSAALGSSFDASVNSPGSPGGCAPTDAGATTIGIDLAGFLAGAHPASGGVWRGDA
                     AGGTVATITYQTVIDASYRCRFGGGAVNSRDVIDNVATVSGRVDVPGGAVVTDGTDDS
                     VTIVAPSTAKSVYAINGDTSDTGPDIAAGDRVTYRITATAPITNVLGLTITDYLPLPT
                     FDIPSGATPFTNLTGSGPVAPGPFSPIAWSVALGPTDTFSAATSSTPTLSVDQTGNSV
                     AMSYPDYQSAPGTTGRTIDLLITVVANDQPFADGLLLTNQARLSSANSPDEVVNQDVI
                     VQVNLTQPVLEITKGVVATDNPAGTFSPAQVGPAGISWSAPGSAGTRFSGGSLTSTAL
                     ATAPVDSNLSRVDAGDTVTFAVVVTNTGSGIRGAFDVAMADVVPAGFSMPAGGWNLAV
                     TDGNGTALAHTGTMPASITLTDPAADRGALTEYDASSGTNLAVVTFDATLDATVAMTQ
                     VLTNQAQVTNFSGSESGPNFVLQPIEDTATVTTAPPTFNKSVIATGIGVTPTAPPNLE
                     LPIGSTAVYRVVSTVPEGQTANFSIRDTLDAGLAFVSFDSITPSAGLSTSHSGGFAGS
                     LADVAVTGSGTVATFPLDTITNSNTDNSTPETITIEYTVVLLNAASNTTGGSRNNSVT
                     GRVGSSNVGSAASAPNIRINEPSLTVTKSASPNAADAGDTITYTVTMAASAATRTITA
                     HDVVLTDTLPSGVDYVAGSFTHTAGVAPSSIDTSAASATWTTFAPGASSTFTLQAVVR
                     DDATSFGPSVINTARATYTSLPGTHSSSLSPYSPLGVERTGSTADPGGAANTYNRTGT
                     STVAVNSSTVTKSLVSTSASHTSGSSVTIGETATFRLDVTVPDGNLGDVTVTDVMPAG
                     LTYVAGSAAIIPGSFAGTWTGSPALPAAQMSGNNVNFVFPNMSVTSGPGTSDNTFAIT
                     LTARVADVPGNAAGTTLPNVGRVTVAGTQFNSVPVNLTVVVPALSVTKTFTPGQAAAN
                     DSVTVGLTVTNTGTSTAYDTVVSDVFDGDVFTSVTPNAVAGWTATAVPQGDDTLVTYT
                     ANSGTALAVSGAQSFSVTATLVGDVTAPATHTNTANASGTTLDGPDVNERTVTASGSD
                     TLDVTGVDIAVTKTADVATADAGEDITYTLTVENLGDRDATGVTLSDTLGSHVALLSA
                     SGSHSVTGNTINWTPFPLAAHATTTRTVTVRVVNPLPVGASSTTNTATAADDHAHGAD
                     TDPSNNSDSALVGLGSVVDLSVDKTGPATVVPGGSISYGVTVTNLGDRNADDATVTDT
                     LGTGLTFVSATGGGTYDPGTRTVTWTGVDLTGTAGSTPSASFTVNATVPDPVPAGRLN
                     VTNGAEVSHPDDVNPLNDSDSVVTAVDADVDLAVTKTDGVTEVVPGDALTYTVSVTNS
                     GSRGAAGVTVVDTLDANHTFVSASNGGTYDPGTRTVTWTLGVVEVGDVVTRTVDVTVS
                     DPLSPLSTSSLTNTAVVSDDGTNGPERDPSDNTATDVDLTGADLAVTKELDSPAVVPG
                     DRAVYSIAVTNHGPMTVADVSVNDTMPTTFSLISASVDVGSIDTATWLWTGVDLAPGD
                     TATLTVTVEVSLDATGDQTNAVTVTGIDVGDPDPSNNSDSVTDPADPTADLSLSKTLE
                     GSLARGATASWLLVVTNHGPSRATAVTVTDTLPSGLTYSGATGVDGANWTCTTTASGT
                     VSCVLDGTMQPGDTAGLRISTTVAADAPAGTVQNRATASAAETETTAANNSAVAAGSL
                     AALPSPVQPSDVASPGGGAAQPSSLPFTGTNPATLLGLGASLIAAGAALVVIRRRRER
                     "
     gene            20617..20820
                     /locus_tag="PLZ72_04290"
     CDS             20617..20820
                     /locus_tag="PLZ72_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35552.1"
                     /translation="MARTVVDIDDDALAAAAVELGTTTKVDTVNRALSLVGSRSERLA
                     VLEALRTADDDLGDAPVMADAWR"
     gene            20811..21239
                     /locus_tag="PLZ72_04295"
     CDS             20811..21239
                     /locus_tag="PLZ72_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA35553.1"
                     /translation="MAVGRHLADKSAFEQQRHSEIADGVLTALAVEGYLCMTEIVAME
                     LLYSCRGPEDYSARWEDLHKLPWLPLDADVATEAISLQRRLAERGQHRQPIPDLLVAA
                     TAIVNDATVLHYDRDFDLLSSISDLSARWIIPPGTGHGGP"
     gene            21251..22582
                     /gene="obgE"
                     /locus_tag="PLZ72_04300"
     CDS             21251..22582
                     /gene="obgE"
                     /locus_tag="PLZ72_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNA35554.1"
                     /translation="MSNFVDECSLNVRGGDGGAGCVSFRREAHTPRGGPDGGDGGSGG
                     DIWLVANHNTASLLAFRDHPHRRAPSGTHGRGAKRHGHKGEDLIVPVPIGTQVFDWEG
                     VPLADLVGDGDRWLAASGGQGGKGNARFLSNKRRAPGFAEQGEHGEERWIRLELKLMA
                     DVALVGFPNVGKSTLISRISAARPKIADYPFTTLEPNLGVVRTDDGFEMVVADIPGLI
                     EGAAEGRGLGHRFLRHIERASVLVLLLDLSPFAGATWSEQEAILLQELGDYRPDLLDR
                     PRLRIGSRADLAEPEVLADFDGPAVSAVTGEGMASIVGALRQLVEKARAEQAPPEGFV
                     TLRPVLEGITISRRDDGSFVVEGREALRAVNLNDLTNPDAMDVARERLDKLGVDRALG
                     RAGARNGDVVHIGALSFDFESDTDMVVQPFDEPDLDDTDLDEVVLDEGTDR"
     gene            22579..23673
                     /gene="proB"
                     /locus_tag="PLZ72_04305"
     CDS             22579..23673
                     /gene="proB"
                     /locus_tag="PLZ72_04305"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNA35555.1"
                     /translation="MRIVLKLGSSSLTDDQGTIDVAAITKTSGEVAAARAAGHEVVLV
                     SSGAVAAGLPALGIPAGDRPRDARTLQAVSAVGQARLMQTWGRHLGDHDLVAGQVLLA
                     PLDFMVRSQYLHARSTLERLLELGVVPVVNENDATADDEIRFGDNDRIAALVAQLVGA
                     ELLVLLTDTPGLMDADPRFSAEASLIEEIAEVDHELEALAGGAGTARGSGGMASKLAA
                     AKMASWSGVRAVIAAAGRSGVLDDAIAGRSGVGTVVLPRARRLSARKLWIAFALPSRG
                     RIVIDDGAVRAVAAGGRSLLAAGVTGVHGDFDADDAVEVVGADGTVVAKGLVRLGATT
                     LRSRAGRRSADLVDGAGEVVHADDLVVLPA"
     gene            complement(23685..24140)
                     /locus_tag="PLZ72_04310"
     CDS             complement(23685..24140)
                     /locus_tag="PLZ72_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35556.1"
                     /translation="MGMYDEFTDAIRDGQIKCWGKRLDLYIIGDPVTLHVAVDDDTAH
                     ELARELDADEDAGLADQPRSMRTRSLLYDGRPRPDILDFQVLCRDGSALVVLDGHWVR
                     WADAPEEGLLLVDNRGRAVEAGDVDRGAWIIDDDHRPRRRRDVAPDDAS"
BASE COUNT         3393 a         8235 c         9161 g         3532 t
ORIGIN      
        1 agccggcccg ctacatcggc tgcgaggacg gcgccgccac ccccgactgg tccgccgccg
       61 gccccaccag cccggtcgtg gcgtggctga tgacctatcc cgacacctac gagatcggtc
      121 tgcccaacca gggcctgcag atcctctacg agatcctcaa cgagcggccc gacgccctgg
      181 ccgagcggtc ctacgccccg tggatcgacc tcgaggccga gatgcgcgcc accggcgtgc
      241 ccctgttcag cgtcgacacg caccgcgacg cgggcacgtt cgacctgctg gcgttcaacc
      301 tgtcggccga gctgacctac accaacctgc tcaactgcat cgacctggcc ggggtcccgg
      361 tccgcgccgc ggatcgtcgg gcgcatcacc cgctggtggt gatcggcggc cactgcgcgt
      421 acaaccccga gcccgtcgcg gcgttcgtcg acctggtcgt gctgggcgac ggcgaggaga
      481 ccgtcggcga catcacccgc atcgtgtcgg actggaaggc gtccgggcgc ggtgaacgcg
      541 aggcggtgct gcgggcgctg tgcgcggtgc ccggcgtcta cgtccccgcc ggctacgagg
      601 cgcactacga cggccaggac ctggtcgcgg tcgagccgat ctggcccgag gcgcccgagg
      661 tggtggagaa gcgctcgatc gcggacctgg ccgactggcc gtacccgcgc tcgcagctcg
      721 tcccgttgac cgaggtggtc cacgaccgac tcaacgtcga ggtgttccgg ggctgcaccc
      781 ggggctgtcg cttctgtcag gccggcatgg tcacccgccc ggtccgggag cgtcccgccg
      841 agcaggtccg ttcgatggtc gcgggtggac tgcgtcgcac cggctacgac gaggtcgccc
      901 tcacgtcgct gtcgaccgcc gacttctccg gcatcggcga cgtggtcggc ggcatcatcg
      961 acgacgaggc gtgcggtcgg gtgtcggtgt cgctgccgtc gctgcgggtc gacgccttcg
     1021 gcgtcgacat cgcgtcgcag ctgcaacggg ggcgtcgcac cggactgacc ttcgcgcccg
     1081 aggcgggcac gtggcggatg cgccaggtca tcaacaagct catccgcgaa caggacctct
     1141 acgacgcggt cgagtcggcc tactcgcagg gctggcggcg gatgaagctg tacttcctga
     1201 ccgggttgcc gaccgagacc gacgaggaca ccctcggcat cgccgagctg gccgccaact
     1261 gcgtcgagat cggcaagcgc caccacaagg gcccgtcggt gacggtgtcg ctcggcgggt
     1321 tcgtgcccaa ggcccacacc ccgttccagt ggttcgggca gaacacccag gtggagctgc
     1381 agcgcaaggt ccacctgctg cgcgacgccg ttcgacgcca ccacggcgtc cagatgaagt
     1441 ggcacgaccc ccgcgccacc ctcgccgagg gcatcgccag ccgcggcgac cgacgtctgg
     1501 cgccggtcat cgaggaggtg tggcgtcggg gcggcacctt ccaggagtgg tccgagtgtt
     1561 tccgcatcga cctctgggaa gaggcgatgg ctgcccacgg actcgacatc gagtggtacg
     1621 tgtaccgcca ccgccgcgag gacgaggtgc tgccctggga tcacatctcg gccggactgc
     1681 acaaggactt cctctggcag gagtggaccg acgccctggc cgaggtcggc ctgcccgact
     1741 gtcgctggac gccgtgctac gactgcggcg cgtgcaccgg ctacgggatc gagcacgtgg
     1801 tcgcgtcggc cacccctccc gccggcggga gccagggcac cggacaggac ctcagcaccg
     1861 gcggcgccgt cccggtgtcg ctcagccccc ggccgacggt gagcgcctga tggaggtcag
     1921 ggtccgctac gccaagatcg gaaaggtgcg gttcctgtcg caccgcgacc tggcccgcat
     1981 cctcgagcgt gccgtgcggc gagcggggct ccccgtcgcc tacagccagg ggttctcgcc
     2041 ccgggcccgg ctgcacttcg gcctggcgct gtcgaccggc tacgagtccc tcgccgaata
     2101 cctcgacatc gacctggtcg agcccgacga gggcggcacg gcgtccgcac tgcccgcggc
     2161 ggaagaggtg gcggacctgc tccagccgtg cctcccgccg ggggtcgtga ccaccgcctc
     2221 gacgtcgatc gaggccggca ccgccgcgtt gcaggacgcg gtgacctcgt gcacctggga
     2281 gttcctcctc gacccggccg accacgaccg ggcgaccgcc aacgccgccc ggatgctggc
     2341 cgccgaggag ctccccatcg acctcgtccg caagagcaag caggtgcgcg aggacctgcg
     2401 accgctcctg ctgtcactcg acgtggaccg cacgcccgaa tgctctcgac tcaccgtcga
     2461 gctgggcacc aaacctcgca gcgtccgacc cgccgagctg ctcgccgcca tggagctgcc
     2521 cgagcatccg atccgggtcg tcaggacgca tcaatggatc cagagcgacg gcgaacgtcg
     2581 cgagccgatc gagctcgctg cgccgtcccc gcacgcctcg gagcgtgcgt catgaaggga
     2641 acgtcccatg tcggacaccg aatcgaaccc accccgcccg accggcggaa ccaccgaacc
     2701 cgccgcctcc ggcgcctcag cggctccggc ccggcccgcc gctgaagcgg ccccccgtcc
     2761 caagatcggc gactcccgcc ctgccccgcc ggttccggcc ggggggtccg gcgcgggcgg
     2821 tccgtccccg acgggagggt cgtccggggg ctcctcgggc ggccagcccg gaacaggcca
     2881 gtcccgcagc agccgtcgcc ggcggcgtcg ccgtggtggc ggcggtggcg gcggccagag
     2941 cggccagggc ggccagaacg cgtccggttc gcagaaccag tccggttcgg gcaagtccgg
     3001 ccagggcaag ggcggccagg gcaagggcgg ccagaaccgc tccaagggcg gccagggcgg
     3061 ccagaaccgc tccaagggcg gccagagcaa gggcggccag ggcaggagcg gagagggtcg
     3121 cagcgcgccg acgccggtcg aggcggtcct gagcgacgac accccggtcg agctcgacga
     3181 cgagaccctc gagcgtcgcc gcggccgcca gcgcaagggc cgggcgatcg gccgctacca
     3241 gatgaacgtc cacgtcggct ccgacgcgac ccacatcgcc atcctcgagg gtcgatcgct
     3301 ggtggagcac ttcgtcagcc gcccctccga cgacgtgagc cagatccacg gcaacatcta
     3361 cgtcgggcgg gtcgagaacg tgctgcccgg catggaggcg gcgttcatcg acatcgccac
     3421 ccccaagaac gccgtgctgt accgcagcga cgtccagtac gaccccgacg acatcgagtc
     3481 gaagggtggc gaccagcccc gcatcgagga cgtgctgaag gcccgtcagc tgatcgtctg
     3541 ccaggtgacc aagaacccga tcgcccacaa gggcgcccgg ctcacccagg aggtgtcgct
     3601 gcccggtcgg ttcgtggtcc tcgtccccaa ctcccggacc ttcggcatct ccaagcgcct
     3661 tcccgacaac gagcgcaagc ggctgcggca gatcctcgac aaggtgaagc cggagcgtca
     3721 cggcctgatc gtgcgcaccg cggccgagaa catcaccgcc gacgagatcg agcgcgacgt
     3781 cgcccgtctg gcccggcagt gggagcagat cgaggcgctc gccgccgagc agcagcgacc
     3841 ggggctcatc taccgcgagc ccgagatggc ggtccggatc atccgcgagg agttcaactc
     3901 cgagtaccgc agtgtcctca tcgacgaccg cgccctgttc gacgacatgc acgactacgt
     3961 cgctgcggtc gccccggcac tcgtggatcg ggtgcagttc ttcgatccgg acaccgagtc
     4021 gctgcccatg ttcgagcggt tccacgtgac cgagcagctg gtcaaggcgc tcgaccgcaa
     4081 ggtatggctg ccctcgggcg gatcgctgat catcgagcac accgaggcgc tgaccgtcat
     4141 cgacgtcaac accggccgca acgtcggctc gtcgtcgctc gaggagacgg tgttccgcaa
     4201 caacttggag gccgccgagg agatcgcccg tcagctccgg ttgcgcgaca tcggcgggat
     4261 catcgtgatc gacttcgtcg acatggaggt caaggagaac cgcgacaagg tcgtcaaggc
     4321 gttccgtgac gccctgtccc gggacaagac ccggacccag gtgttcaaca tctccgagct
     4381 gggcctggtc gagatgaccc gcaagcggat cggcgaggga ctgctcgagt cggtggccga
     4441 tcgctgcgac cactgcgacg gtcgcgggct gctcctgccc gacgagctgg tcggctgatc
     4501 gcgggagatc ccggaatcga ctaccggtca ccgtcggtgt tcccctaagc tggcgagccg
     4561 tatgtatgca gtcgtgaaga ccggtggaaa gcagtatcga gtggaggagg gccagcggct
     4621 ctccgtcgag cggctcggcg atgccggcga cgacgtccag ctccggccgg tcatggtggt
     4681 cgacggcgac accgtcctgg ccacccccga ccagctctcg ggcgcctcgg tgaccgctcg
     4741 ggtcgtcggt gaggccaagg gtcccaagat caacgccttc acctacaaga acaaggcgaa
     4801 cgagcggaag cgctggggtc accgccagag ctaccacacc atcgagatca ccggcatcac
     4861 cgccggctga cggaatccac gcacagagga cgaggctcag atgtccaaga ccaagggcgg
     4921 cggctccacc cggaacggcc gtgactccaa cgcacagcgc ctgggcgtga aggtgtacga
     4981 cggcggtgtg gtcaccgccg gttccatcat cgtccgccag cgcggcaccc gcatccaccc
     5041 gggcgagaac gtcggcatcg gcggcgacga cacgctgttc gccctcgccg acgggaccgt
     5101 caagttcggt tcccgccgca accgcaagct cgtcaacgtg gtgccgctcg ccgactgagc
     5161 ggaccccatc ggtcctgagc gacgcccgcc cctcggggcg ggcgtcgtcg cgtccggtgg
     5221 ccgggcgtgg ggttcagccg atgccgacgg gcagctcggc gtcgggccag cgcagcagcg
     5281 tgcgggcctg gtcgaacgcg aaccggtcgg tcatgccccc cacgtacgtc accgcctggc
     5341 ggaacgcctc ggtgctgccc ggttcgacgc cgtggagctg ttcggcgggc ctgggcaagg
     5401 cgtggggtcg gtcggcgtgg tgctcgacga gggcacgcag cacccggatc acggcctggg
     5461 actgctcgac cgatgccggc cgcatgtaga tgtggtcgta gttgaaccgt cggaactcgc
     5521 cgagagcttc ggccagaggc tcggtcatgc ccaccgaacc ggtggcggcc accgcgtcga
     5581 cgacgccgcc gatgaacgag ccgagctgct ggctgtggcg ggtcccgcag cggtcgatca
     5641 cgaccggagg caggagttcg gcggcggtga tgccgacgtg gacggcgtcc tcgaagtcgt
     5701 ggcacacgta cgcgatgcgg tcggcccacg acacgacctc accttcgggc gtggccggcg
     5761 ccggtcgcga ccacgagtgg ttgcggatcc cgtcgagcgt ctcggcgcac aggttgagcg
     5821 gctcgaggac gacgtcggcg ccccagacgg cgtggtcgta gccgccctcg acgtaggggg
     5881 cgaacgcgtc ctcggacgcg tggccgccgg ggccgtggcc gcagtcgtgg ccgagcgcga
     5941 tcgcctcggt cagcgcgacg ttgagcccga tcgcccggct gatggcgacc gccacctgcg
     6001 ccacctcgag cgcgtgggtc atgcgggtgc gctggtggtc gtcggggaac acgaacacct
     6061 gggtcttgcc ggccaggcgc cggaaggcgg tgccgtgcag gatccggtcg cggtcacgtt
     6121 cgaagcaggt ccgccagtcg tcgggcgcct cgtcccgcgc ccggtctccc gccccggagg
     6181 cgagcgtggc gccggcgacc agcgacaggc cctcggactc ctcgcgggcg gtgcggccga
     6241 gcgggctgac ggggccgacg acaccggtcg gtccgatgtg ggcgtggcgc accgcggagg
     6301 agtcggagct ccggtggcga tgacccgaca tggtcgacac ccactcctcg gcgcgcgacg
     6361 gatcgaccgc tgggggagtg ggggtggatc gctccggagt cacggaccga gcctacggcc
     6421 gccgggtgac agctcccggt gccggggcgc ggggctcacg gccaggggac cgcggcgtac
     6481 tcctcgcgca gcttgtactt gagcaccttg cgcagcgtct cgttgcgggg cagggcgtcg
     6541 acgatgacga gctgctcggg gaacttctgc ttcatcagct gggcgtcggt cagcgtggcg
     6601 accatctcgt cgaaggtgag gtcgtcggcc gggtcggcct cggggacccg ctcgacgacc
     6661 gcgcacaccc gctcgccgcg ctcgcggtcg ggaagcccga tcaccgcgac gtcgccgacc
     6721 ttggggtggg cgtagagcac gtcctcgatc tccttggccg agatgttctc gcccttgcgg
     6781 atgatcacgt ccttgagccg gccggtcagc acgacgtggc cgtcggggcg cacgtggccc
     6841 aggtcgccgg tgcggaacca cccgtcgtcg tcgaacgcct cggcggtggc ggccgcgtcg
     6901 gtgtagccct tgaacaccat cggccccttg aggcggacct cgccgtcctc gccgggtccg
     6961 gcgaccgagc cgtcctcggt cacgatccgc aggtcgagcc cctcgaccgg cttgccgacg
     7021 gtgtgggcca gctgctcgtc ggtgtcgtgc ggtgagccct cgcagatcat ggggcattcg
     7081 gtcatgccgt agccgtggca gatcttgacg ccgaactccc gcttcacctc gtcgaagatc
     7141 tccgggggca tgggggcgcc gccgccggac atcttgcgca gcgacgggat gatcggcgtg
     7201 gcgggatcct tgcgctgctc ggtcaggtac atctggtaga acgcggtgcc gccaccgacc
     7261 atggtcaccc cgttggcggc caggaccggg atcgcggaga ccgggttgaa cgcctcgaac
     7321 aggacggctg ggaacccggc gagcagcatg gtgccgaagt agtccggtcc ggcgatgtgg
     7381 ctgaacggga aggcgatcga cccgacgtcg tcggggctca tgtcgagcgc gacggccagc
     7441 ccgttggcgc cggtgatgag tgtccggtcg ctgtggcgca cgcccttggg gtccgacgtg
     7501 gtgccggagg tgaagtacac ccagcggacc tcgtcgccgt cggtgggagc agcgacgccg
     7561 gcgagggtgg cggggtcgcc gtcgggcagg tccgcgaaga cgtcgatcac cacgggggga
     7621 gcggcggagt cggcggtcag ctcgtcgacc atggccgcga agtcgaagcc ggcccactcg
     7681 ccggggacga gcacgaactc ggcctcggtg gcccggatcg cgtagccgac ctccttgtgg
     7741 cggtagaagt gcagcaccgg gttctgcacc gcgcccaggc gggccagggc gaagctggcg
     7801 acgatcgtct cgaaccggtt gggcagctcc caggtgaccc gggtgccgtg ggtgacgccg
     7861 agggcgtgga acccggcggc ggcccgcagc gcccggtcgc ggaactcgcc gcaggtcagg
     7921 gtgcggcccg actcgtcgat gagcagcagc gcgtcggggg tggcgtcggc gcggcgctcg
     7981 acgagctccc agagcgtggt ggtgtcagcg aaggtcgtgg ccatggttcc ctcctgggcg
     8041 gcggcgcttg cgacactacc gatccgcggg agcggacgtg aacgaccgtt cggcccgctc
     8101 ctcggcctcg atcccgtctc gtggcctcac accgagacag gggaaccttc cgttgcgctg
     8161 cgagcggcag cgatgagcat cgggcggctg aacgaggcgc tcgccaaacc catggcgatg
     8221 gcggcgacca cgatgaggcc gagcaccccc acgttcggcg acgcgagtcg ggccgcgctc
     8281 agtccgaacc cgaccatcag caggaacgag ctgcccaggc cgatgacgac cgatccgatg
     8341 gtggcgaccg cgaggggcaa caccgtctgg agtcgccgtg cccgttggag ggtgctcacc
     8401 tcggtgccgc acaggaccag gcgcccgatg aggcggcgtt ggtcgaccac cgcagcggcc
     8461 gcggcgaccc ctgacgcacc gaaggccagg agcaggccgg acgccagcac cgcgaacgat
     8521 ccccgcatgg catctgcggt gaacgtgatg tcgccgaaga ggtcgtcgtc ggatgcgacg
     8581 agcggtgtgc cacctctgat ggcggtggtg gcggtccgga tccgctcgag tcggggcccg
     8641 ctcgcgagca cgacgacgtc gaccggttcg ctcacctcgc cacccggctg cgtgtcgacc
     8701 ggccgctcct cgcccgctgc ggcgagcaca gcgtcggcgg ccgggcggtc caggacggct
     8761 gtcgccgcgc cgctcggcaa cccgtcgcgc tccagcgcct gcaccgtcgg catcaccccg
     8821 gcgaggaacc ccgcggtcag cccggcgagc accacggcgc tcacggtccg gtaggcggcg
     8881 cgcgggtctt cgagcacccg tcgacctgcc agcagcagcg gtgtgcgtcg ggccgagcgg
     8941 accatgatcc ggccgacgat cgacgtgacc cacgggccga ccaacgctaa cgacgcgatg
     9001 accgtggcga gcgcggcgac catcggtacc gacatgtcct tcggctggcc gttcgaaccg
     9061 gtgaaggtct gtgctgtcgc cgcaacggtg gtgacgcaca gcagcgcgat cgccgccagc
     9121 aggccgagca cgcgtggcca tcgtgccgac gcgggttcgg ccgaagcggc cacgccgagc
     9181 ggccgtgacc ccacccgtcg caacgagacc gccgtcgccg ccgcgcacac ggcgatggtc
     9241 gcgacgaaca gtgcggcggc gctcgatacg gggagtcgga tgtcggcggg gaaccatcgg
     9301 ccgccggcca tcgcgatgtc gacgagcaac ccagagaccg ccatcgcgga ggcgatcccg
     9361 atgacggcgc cgagcgccgc gccgatggtc gtctcggcaa ccgcgagcag ccgcaccgcg
     9421 ccggaggtgg caccggccag gcgcagcgaa gccatgcgct gctcgcgtcg cgccgcggtc
     9481 aacctggccg aggatccgat gagggcgacg gcgggcacca cgacgaggac gacggcgacg
     9541 agtgcgaagc tccggtacat catcaggtcc cggtcggcac cggtcgtgtc gaagctcgcg
     9601 accggaacgg tgtcactcgg acgatccgga ccgagcacgg cgttcggtga cccagagctc
     9661 gtcgccgggc cgagctggtc cggggtcgtt ccgaccaccg cgacgagctc atcggggtga
     9721 gcgacaccgg ctgctccgat gcggccgacg acttggccga atcggtcccc gagctggtcg
     9781 ctcgggtgcg aggcgatgag ctcggcgagc gcgggagaca cgcatgccgt cccgggagcg
     9841 ggcgcggcgc ggagtccggg cggcgcgggg gcctggtcgg tcgtgtccaa cgccgcgatg
     9901 tcgacacggt cgatgcgtcg accgtcgaac cagtcggtgc tccgacgttg gagcgccacc
     9961 ggagacacgt gcgcctcggc agcgggcgtc gggctccgcc agaccacctt gggttgacgg
    10021 gcgccgaagc cgtggtaggc gccgagggcg acgccggcga cgagcgtggc gatggccacg
    10081 ccgactgcgc agagcacgat cgctcccgcg gactggcggg gcgagcggcg caacaggagc
    10141 ggcaacagcc gcagtcccgg gctcacccga ccgaccctgc ggtcgcggtg gacgacgccg
    10201 acgcagcgga cgggttggtc acgaccccgt gcacccgacc gtcgcgcatg cggaccgtgc
    10261 ggtcgcacga cgccgccaca tcgaggtcgt gggtcacgac gagcagcgcg gcgccggcgt
    10321 ggcggcacgc acgggtcagg acctccatcg tcgtggtgct ggtcgcacgg tcgagagcag
    10381 cggtgggttc gtcggcgaag accaccgacg gcccgacgac gagcgccctg gctatcgaca
    10441 cccgctgcac ctgaccgccc gacatctgac ccggtcgacg ggtctcgagg ccggcgaggc
    10501 cgagcggggc gaaccactcc gcggcccggg cggtcgccga tcgccgggac atgccgccga
    10561 gcatcagcgg cagggcgacg ttctccacgg cggggagctc gcccagaagc tggtcggact
    10621 ggaacacgaa cccgaaccgc tcccggcgga gcttggtcgc ggtgcgctgc ccgagccgtg
    10681 agatgagcaa gccgtcgagg gtcacgtccc cggcgtcggg ccgggtgaag ccggcgaggc
    10741 agtgcagcag ggtcgacttg cccgaaccgc tcggacctac caccgcggtg gcggtgcccg
    10801 cctcgaggga gatcgacacc ccagcgagag cggtcgcgtc gcggtatcgc ttggtcaggc
    10861 cggtggcgac gagcaccggt cccggtcggg acggcggtga ccacggtgac ggaggggact
    10921 gcatggggtc gagctcagga gcggtaaggg gcatgaggtc gatggtcggc gttcgacggt
    10981 ctcggcgcat cggcctgacg gtcgcttcct cccgaccgat cggccggggg tgggtcgccc
    11041 aacaccaacc gatcggccga ggtggccgca gcgggggccg acgtagcatc gagcggtgga
    11101 ccagcacggc ctacccaccc ggacccggac ggcgcccgtg ccgtcctcga tcgatcgaat
    11161 cctcgacagg gcgcagcagg ccgctccatg gatcgaccgg gccggtgacc tgctgctcgt
    11221 cctctgctgc gccgggttca gcctgctcga cctggcgatc ctgctcacct cgggacgctc
    11281 ggagacctgg ctaggcgtcg ggttcggtgc tctgggcgcc gtcgccctcg gcggtgtgag
    11341 cctggtcgcc gtcgcagcgg tggcgttgcg ccgacagctg ggtctgatcg cgctcgtggg
    11401 gatgagcgtc ctgtcgctct gcgcgacgac ggcgtcgctg ttgttgggcg tgtcgctgcc
    11461 cccgtcgttc gcggtgctgt tcgccctggc ggtcctcgtc gcccgggctc ttcacgtgga
    11521 gcccgagctg ccggcggccg gggctgccct cctggcgctg ctggcggtcc tgggcgagcc
    11581 ggcccgcctc caggaccgcg gcaccgtcga gcccatgcag gtcctgtgcc tgctcgggct
    11641 cggtggcgcg gtggtcgttg gtctgtggct gcggttcggg gactggcggc gggctagcaa
    11701 cgaggcagcg gtccgctccg acgagcggtt ggcgctggcc cgtgagctgc acgacctcgt
    11761 cggccaccac gtcacaggga tggtggtgca ggcccaggcg ggacagcttg tcgccgagag
    11821 cgacccggcc gccgcggtgg agtccttcgc ccgcatcgag tcggcgggcg ccgatgccat
    11881 gtcagcggtg cggcggatgg tgggtgacct gcgcgacgac gcaccgcgcg caccgggggc
    11941 cggctgggac gacgtcgacc gcctgatcgc ggacgcggtc ggcgacggcg tcccggtccg
    12001 ctgctcggtc gaggccgcag cccgacgggt tccgccggag ctgacctcgt cggtgcatcg
    12061 gatcgtggcc gagtcgttga cgaacgtccg tcgccacgcc cgggccgtca cgaccgtcga
    12121 tgtctcggtg cgggtcgagg acggtgacct ggtcgtcgac gtccacgacg acggcgctcc
    12181 atcggtgcat ccggccgggt cgtcacgccc cgacacgttc ggcctggtcg ggatgcgtga
    12241 gcgcacggag tcgctgggcg ggcggttctt cgccggggcg gcaccagggg gtgggtggct
    12301 ggtctcggcg cgtctgccgg tcgccgccgg cgaacgcgtc cgtcgccgat gatccgggtc
    12361 ctcctcgccg acgaccagga gatggtccgg gtggggttcc gcatgatcct cgactcggat
    12421 gcggagatcg aggtggtcgg ggaggtgccg gacggcgccg acgcagtccg actcgcccgg
    12481 gagctcggcc cggacgtggt gctgatggac gttcggatgc ccggcacgga cggcctggag
    12541 gccactcggg agatcgtcga aggtgcagtg acagcccgaa ccggggatcc ggtccgcgtg
    12601 gtcgtggtga ccaccttcga cctcgacgag tacgtcgacg cggcgatcac caacggtgcc
    12661 gccgggttcc tgctcaagga ctccggaccc gcgctcctga tcgaggcggt caaggcggcg
    12721 gtgcgcggcg acgtgctgat ctcgccgtcg atcacggtgc ggctgctgtc gcacttcgcg
    12781 ggtgtcggat cgactgcagc ggggccagcc ggcgcgatcg gatcgccgcg gtcggctcag
    12841 ccggtcgtgg ccctgaccga acgcgaggag gaggttctgt gcgaggtggc gaaggggctg
    12901 accaacgccg agatcgcgga cgtcctcttc atctccctcg gcacggtgaa gacccatctg
    12961 gggagcatgc aggacaagct gggggcacgg aaccgcgtcg agctcgccgc atgggcgtgg
    13021 cgcaccggac gcatgtcctg agtcccgatc cggtcgacgg cgagcgcttg gtacggtcac
    13081 gcggtgacgc caccgatccc gcatgctcgc cggtcccggg tccgctcggc cgtcgccccg
    13141 tgggccgcgg cgctgacccg tcggtcctac cccgtgctcc acgcggtggc gaccgcggcg
    13201 gtggccgtcg gtcgcggcgc cctgcactgg gcgagcctcg gaccgggcga cgccggccgg
    13261 gtcggcttcg ccgctttcgg tgagcggtcg ttcatccgcc agccctactc cgacatcgtc
    13321 ggcgcgcccg ggatcgccat cggtgccgac acgctgatcg gctccggcgt caacctggcc
    13381 gcggtgcccc aggacgagtg gtcacccggt gacgagccgc tgctgcggat cgggtcgcgg
    13441 gtgtgggtcg ctcccggcct gcacgtcgtc gctcaccgcc gcgtggagat cggcgacgac
    13501 gtgtggatcg gacccggcgt gtacatcacc gacgccgacc acgaccgctc cgatcccgac
    13561 gtcccgatcg gtcaccgcat ggccccggcc cgaccggtgc gcatcggggc ggggtcgtgg
    13621 ttgggtaccg gagtggtcgt gttgtcgggc gtcacgatcg gcgagcgcgt caccgtcggg
    13681 gcgaactcgg tcgtctgcga cgacctgccg gacggttcgg tggcggtcgg cgccccggcg
    13741 cgggtggtcc gtgaggcgtc agcgctcccg tcggcggcgg atgacgacca gcgccgcgcc
    13801 ggcggcgatc agtgacgcac cgagacccag gagcgttgcg gggttcgttc ccgtgaacgg
    13861 caggctggac ggctgagcgg cgccgcctcc gggggacgcg acgtccgacg gttggacggg
    13921 ggagggcagc gcagccaggg agccggcggc gaccgccgag ttgttcgccg cggtggtctc
    13981 ggtctcggcg gcggacgccg tcgcccggtt ctgcacggtc ccggcggggg cgtcggcggc
    14041 cacggtggtc gagatgcgca ggcccgcggt gtcgcccggc tgcatcgttc cgtcgagcac
    14101 gcaggacacg gtgcccgagg ctgtcgtcgt gcaggtccag ttcgccccgt ccaccccggt
    14161 ggctccggag taggtgaggc cgctcggaag ggtgtcggtg acggtcaccg cggtggcccg
    14221 cgagggaccg tggttggtga ccaccagcag ccaactggcc gtggcgcccc gggcgaggga
    14281 cccctcgagc gtcttcgaca ggctcaggtc cgcggtcggg tccgccgggt cggtgaccga
    14341 gtcggagttg ttcgacgggt cggggtcccc gacgtcgatg ccggtcacgg tcactgcgtt
    14401 ggtctggtcg ccggtggcgt ccaggctcac ctcgacggtg acggtcaacg tcgcggtgtc
    14461 accgggggcc aggtccacgc cggtccacag ccaggtggcg gtgtcgatcg agccgacgtc
    14521 gaccgacgcg gagatcagcg agaaggtcgt cggcatcgtg tcgttgaccg acacgtcggc
    14581 gacggtcatg gggccgtggt tggtcacggc gatcgagtag acggcccgat caccggggac
    14641 gacggcgggg gagtcgagct ccttggtcac cgccaggtcc gctccggtga ggtcgacgtc
    14701 ggtggcggtg ttgtccgacg ggtcccgttc ggggccgttc gtgccgtcgt cggagacgac
    14761 cgcggtgttg gtcaacgacg acgtgctcag cggcgacagc ggatcgctca ccgtcacgtc
    14821 cacggtgcgg gtgacgacgt cgccgacctc gaccacaccc agggtccagg tcacggtgcg
    14881 ggtccccggg tcgtaggtac cgccgttcga cgccgagacg aaggtgtggt tggcgtccag
    14941 ggtgtcgacg acggtcacac ccgccgcgcc ccgcgaaccg gagttggtca ccgagacggt
    15001 gtaggtgagc gcgtcgcccg ggacgacctc ggtgaccccg tcggtcttcg tgaccgccag
    15061 gtcgacgtcc gcgtccacgg ccgtgacgac cgagtccgag tcgttgagcg ggttgacgtc
    15121 gtcggggtgg ctgacctcgg cgccgttggt gacgttcagg cggccggccg ggaccgggtc
    15181 ggggaccgtg gcgttgacgg tgaacgacgc cgaaggggtc gatccggcgg tgccggtcag
    15241 gtcgactccg gtccaggtga ccgtccgcgt ccccgggtcg taggtgccgc cgccggtggc
    15301 cgacacgaag gtcagaccgg tgccgagcgt gtcggtgacg gtggcgtcgt ccgcgttgcg
    15361 gtcacccagg ttggtcaccg tcacgccgta ggagatcgat ccgccgggga cgacggtggc
    15421 gggtccggtc ttgtcgacgc tcaggtcgac gaccgaaccc agtccgacca gggcgctgtc
    15481 ggagttgttc gacgggtcgg tgtcggcccc gtgggcgtgg tcgtcggccg cggtcgcggt
    15541 gttcgtggtg ctcgacgcgc cgaccggcag cgggttcacc acgcgcacgg tgacggtccg
    15601 ggtcgtggtg gcgtgagcgg ccagcgggaa cggcgtccag ttgatcgtgt tgccggtgac
    15661 gctgtgggag cccgaggccg acagcagcgc cacgtgactg ccgagcgtgt cgctcagcgt
    15721 caccccggtg gcgtcgcggt cgccgaggtt ctccaccgtc agcgtgtagg tgatgtcctc
    15781 accggcgtcc gcggtggcga cgtccgcggt cttggtcacc gcgatgtcga cgccggtgac
    15841 gtcgagcgtg tcggagccgc tggcggtcac ggtgcgctcg ttgacgtccg ggccgtccag
    15901 ggtggtgccc gacgcgttcg ccgtgttggt gtgcgtcgcc ggagcggtga cgtcgccgac
    15961 cagggtggcg gtgacgctga aggactgggc gccggacacc gcgagggcgg tgcccgagtt
    16021 ggcggtgtag gtcaccagcg tgtcgtcgcc ctgtgggacg gccgtcgcgg tccaacccgc
    16081 caccgcgttc ggggtcaccg aggtgaacac gtcaccgtcg aacacgtcgc tgaccacggt
    16141 gtcgtacgcg gtggacgtgc cggtgttggt gacggtcaac ccgacggtga cgctgtcgtt
    16201 ggcggccgcc tgaccggggg tgaaggtctt ggtcaccgac agggccggca cgacgacggt
    16261 caggttgacc ggcaccgagt tgaactgggt gccggccacg gtgacccgcc cgacgttggg
    16321 cagcgtcgtg cccgcggcgt tgcccggcac gtcggcgacc cgggcggtga gggtgatggc
    16381 gaaggtgttg tcggacgtgc cgggacccga ggtcaccgac atgttcggga acacgaagtt
    16441 cacgttgttg cccgacatct gtgccgcggg cagggccggg gacccggtcc aggtcccggc
    16501 gaacgatccg gggatgatgg ccgcggagcc ggccacgtag gtcagaccgg cgggcatgac
    16561 atcggtgacg gtcacgtcgc ccaggttccc gtcggggacg gtgacgtcca ggcggaaggt
    16621 cgcggtctca ccgatggtga ccgaggaccc gctcgtgtgg ctcgccgagg tcgacaccag
    16681 cgacttggtg accgtggacg agttcaccgc gacggtcgag gtcccggtgc ggttgtaggt
    16741 gttggccgcg ccgccgggat cggcggtcga gccggtgcgc tccacgccca gggggctgta
    16801 cgggctgagc gagctcgagt gcgtgccggg caggctggtg taggtggccc gggcggtgtt
    16861 gatcaccgac gggccgaacg aggtggcgtc gtcgcgaacg acggcctgca gcgtgaacgt
    16921 ggagctggcc cccggggcga acgtcgtcca ggtcgccgat gctgccgacg tgtcgatcga
    16981 actcggggcc accccggcgg tgtgggtgaa cgagccggcc acgtagtcga ccccggacgg
    17041 cagcgtgtcg gtgagcacga cgtcgtgggc ggtgatcgtc cgcgtcgcgg cgctggcggc
    17101 catggtgacg gtgtaggtga tcgtgtcgcc cgcgtccgcg gcgttcggcg aggccgactt
    17161 ggtgacggtg agcgatggct cgttgatgcg gatgttgggc gcgctggccg cgctgcccac
    17221 gttcgagctg ccgacgcggc ccgtcaccga gttgttgcgg gacccaccgg tggtgttcga
    17281 ggcggcgttc aacaggacga ccgtgtactc gatcgtgatc gtctcgggcg tcgagttgtc
    17341 ggtgttcgag ttggtgatcg tgtcgagggg gaaggtcgcg accgtgcccg agccggtcac
    17401 cgccacgtcg gcgagagatc cggcgaaccc gccggagtgc gaggtcgaca acccggccga
    17461 cggggtgatc gagtcgaacg acacgaacgc caggccggca tccagggtgt cgcggatcga
    17521 gaagttggcg gtctggccct cggggacggt gctgaccacg cggtacaccg cggtcgagcc
    17581 gatcggcagc tccaggttgg gcggtgcggt cggggtcacg ccgatgccgg tggcgatcac
    17641 cgacttgttg aacgtcggcg gcgcggtggt gaccgtcgcg gtgtcctcga tcggctggag
    17701 cacgaagttc gggccggact ccgagcccga gaagttcgtg acctgggcct ggttggtgag
    17761 gacctgggtc atggcgacgg tcgcgtcgag ggtggcgtcg aaggtgacga ccgcgaggtt
    17821 ggtgcccgag ctcgcgtcgt actcggtgag agccccccgg tccgcagcgg ggtcggtcag
    17881 cgtgatcgac gccggcatcg tgccggtgtg ggcgagcgcg gtgccgttgc cgtcggtgac
    17941 ggccaggttc caacctccgg cgggcatcga gaacccggcg gggacgacgt cggccatcgc
    18001 cacgtcgaac gcaccccgga tcccggaacc ggtgttggtc acgacgactg cgaacgtgac
    18061 ggtgtcgccg gcgtcgaccc ggctcaggtt cgagtccacc ggggccgtgg ccagcgcggt
    18121 cgatgtgagc gacccgccgc tgaaacgcgt tccggcgctg ccgggggccg accaggagat
    18181 cccggccgga ccgacctggg cgggggagaa cgtcccggcc gggttgtcgg tggcgacgac
    18241 gcccttggtg atctcgagga ccggctgggt caggttgacc tggacgatga cgtcctggtt
    18301 gacgacctcg tcgggggagt tcgcgctcga cagccgggcc tggttggtca gcagcagtcc
    18361 gtcggcgaat ggctggtcgt tggccacgac ggtgatcaac aggtcgatgg tgcgaccggt
    18421 agtccccggc gcgctctggt agtcggggta gctcatcgcg accgagttgc cggtctggtc
    18481 gaccgacagc gtcggcgtcg acgacgtcgc ggccgagaac gtgtcggtcg ggcccagggc
    18541 gaccgaccat gcgatcgggc tgaacggtcc gggtgcgacc ggcccggatc cggtgaggtt
    18601 ggtgaacggc gtggcccccg acgggatgtc gaaggtcggc agcggcaggt agtcggtgat
    18661 cgtcaggccc aacacgttgg tgatcggtgc cgtggccgtg atccggtagg tgacccggtc
    18721 gcccgcggcg atgtcggggc cggtgtcgga ggtgtcgccg ttgatggcgt acaccgactt
    18781 ggcggtcgac ggcgccacga tggtcaccga gtcgtcggtt ccgtcggtga cgaccgcgcc
    18841 cccgggtacg tcgacgcggc ccgacacggt ggcgacgttg tcgatgacgt cccgggagtt
    18901 gaccgctcca ccaccgaatc ggcagcggta ggaggcgtcg atgacggtct ggtaagtgat
    18961 cgtcgcgacg gtgccgcccg ccgcgtcgcc ccgccagacc ccgccggagg cgggatgggc
    19021 gccggccagg aagccggcca ggtcgatgcc gatcgtcgtc gcgccggcgt cggtcggtgc
    19081 acagccgccg ggggagccgg ggctgttgac cgacgcgtcg aaggagctgc ccagagcggc
    19141 cgagccggat ccgtcggggg tgcggtacga gatggtcggc gagaagccca catcgagcgt
    19201 ctggccgtcg gcggcgatgt cgtcgagcgc caggtcctcg aagtcgaagt agtccgacac
    19261 ctggacctgg atggtccagg tgagggtgtc gccgggagtc gggccggcgg gtccggtgtc
    19321 ggtggcgacg accaccgact tctgggtggc gaccgaccgg gcggtgatcg tgtcgagcgg
    19381 cccggccggg tccgccaccg cgttgtcggt tcctgcgggg tcgcgggggt cgagcggggt
    19441 ccagttgccg accgccgacg cgtcgttgct gaccgtggtg gccgcgccgc cggtggggtc
    19501 gatgacgggg ttcccggccg cgtccagctc gggcacccag aaccccacgt cgtaggcggc
    19561 atgcgtggtg gcgctcgggt aggtgacggt gaccgactcg gcggcccacg ggccgggccc
    19621 ggcgggctgg tcggtgaccg tccccgacgg ggtgatcgag ctgatgccgc tgtacgcgat
    19681 gtcgtcggga agggcgtcgg tgacccggag gttggtgacc gtctggccgg gagcgaggtc
    19741 gaccgccacg ttccacagcc gaccgaagtt cgggccggtc gccgtctcgg actcgggtcc
    19801 gttgtcctgc ttggtgatgg tgatgagcga cggggtgacc gagctcgaca cgttcgcgcc
    19861 gatgatcggc gggtcggtca gcgggttggc cagagggtcg gcgccgaagc ggaacccgcc
    19921 gcgggcgacg atcggcagcg tgtggcccag atccgcgaag ttcgacagct gggtgagcac
    19981 cgacagcgtc gccgccggtt gggtcggggt gaaggacccg aacggaaggg tgagcgtcgt
    20041 catcgtgtcg cccgcggtga acccggccgg gcacgtgacg ggctgaccgg tcagcggatg
    20101 gacgtcggag ccgtcgcagg ccaggacggt caccgtggag gggacggtcg cccccaggta
    20161 ggtggccgag gtgaacgaca cgccgtcgcg gggtccgccg ccggacgtgc cgtcggcgcc
    20221 gttggtcggc aggaacaggt cggtgaacgg gccgtagccg gtgtcggccg gcgtcgggtt
    20281 cgagaacgtc agatcgatcg gcacctgctc gccgatgaag ggggtggtgt ccaggtcgag
    20341 gccgaccacc gggagcggcg gtgccgcggc cgccggtcgg gtcccggtgt ggaccaggag
    20401 cgagccgatc accagcgtcg ccgccaccgc ggcgaggagc ttgggtgcga tgcggcggaa
    20461 ccggagccag gacgcggaga agcgcggaac gtcaaacgtc aacatggtgc cctcatcggc
    20521 ccgactcagc ggatgatgag cagtctgcgt ggcgtgagga gccggacgga tgtcgatatc
    20581 atccatgcca ggcgttgata tcgattcgga ggacgaatgg ctcgcacggt cgtggacatc
    20641 gatgacgacg ctctggccgc tgccgcggtt gagctcggga ccaccaccaa ggtggacacc
    20701 gtcaaccgcg ccctttccct ggtcggatca cgatcggaac ggctggcggt gctcgaggcg
    20761 ctgcgaaccg cggacgacga cctgggtgat gcaccggtca tggccgacgc gtggcggtag
    20821 gtcgccacct cgccgacaag tccgccttcg agcagcagcg ccacagcgag atcgccgacg
    20881 gagtgctcac ggcgctggcc gtcgaggggt acctgtgcat gaccgagatc gtcgccatgg
    20941 agctcctgta ctcctgccga ggaccggagg actacagcgc gaggtgggag gatctccaca
    21001 agctgccgtg gttgccgctg gacgcggatg tggccaccga ggcgatctcg ctgcaacggc
    21061 gactcgcgga acggggtcag caccgccagc cgatccccga cctgctcgtg gcggcgaccg
    21121 cgatcgtcaa tgacgccacc gtcctccact acgaccggga cttcgacctc ctcagctcga
    21181 tctcggacct gtcggctcgc tggatcatcc ctccggggac cggccacggc ggcccatagg
    21241 ctggggccct gtgtcgaact tcgtggacga gtgctccctc aacgtgaggg gcggtgacgg
    21301 cggcgccggc tgcgtgtcgt tccgtcgcga ggcccacacg ccccggggcg gccccgacgg
    21361 cggtgacggc ggcagcggcg gcgacatctg gctggtggcg aaccacaaca cggcgtcgct
    21421 gctcgcgttc cgggaccacc ctcaccgtcg ggcaccgagc ggcacccacg gccggggcgc
    21481 caagcgccac ggtcacaagg gcgaggacct gatcgtcccg gtgccgatcg gcacccaggt
    21541 cttcgactgg gagggcgtcc cgttggccga cctggtcggc gacggagacc gttggctggc
    21601 cgcgtccggt ggccagggcg gcaagggcaa cgcccggttc ctgtccaaca agcggcgcgc
    21661 acccggcttc gccgagcagg gcgagcacgg cgaggagcgc tggatccgcc tcgagctcaa
    21721 gctcatggcc gacgtggctc tggtcgggtt ccccaacgtg ggcaagtcaa ctctgatcag
    21781 ccgcatctcc gcggcgcgac ccaagatcgc ggattacccc ttcaccacgc tggagcccaa
    21841 cctgggcgtg gtgcgcaccg acgacggctt cgagatggtc gtcgccgaca tccccggcct
    21901 gatcgagggc gccgccgagg gccggggcct gggccaccgg ttccttcgcc acatcgagcg
    21961 ggcgtcggtc ctggttctgc tgctcgattt gtcgccgttc gccggtgcca cctggtccga
    22021 acaggaggcg atcctgctgc aggagctcgg cgactaccga cccgatctgc tcgaccggcc
    22081 ccgactgcgc atcggatccc gggcggatct ggccgaaccc gaggtgctgg ccgacttcga
    22141 cggtccggcc gtgtccgcgg tcaccggcga gggcatggcg tcgatcgtcg gagcgctgcg
    22201 ccagctggtg gagaaggccc gggccgaaca ggccccgccc gagggcttcg tcacgctccg
    22261 accggtgctc gagggcatca cgatcagccg ccgcgacgac ggcagcttcg tggtcgaggg
    22321 tcgcgaggcg ctccgggccg tcaacctgaa cgacctgacc aaccccgacg ccatggacgt
    22381 cgcacgcgag cggctcgaca agctcggcgt ggaccgtgcg ctgggccgtg ccggggcccg
    22441 caacggcgac gtcgtgcaca tcggtgcgct cagcttcgac ttcgagtccg acaccgacat
    22501 ggtcgtccag cccttcgacg aacccgacct cgacgacacc gacctcgatg aggtcgttct
    22561 ggatgagggg accgaccggt gaggatcgtg ctcaagctgg ggtcgtccag cctcaccgat
    22621 gatcagggca ccatcgacgt cgcggcgatc accaagacca gcggcgaggt cgccgcggcc
    22681 cgcgccgcgg gccacgaggt cgtgctcgtc tcctcgggtg ctgttgctgc gggcctgccc
    22741 gccctgggca tccccgccgg ggaccggccc cgtgacgccc ggaccctcca ggcggtctcc
    22801 gcggtcggtc aggcccgcct gatgcagacc tgggggcggc acctcggcga tcacgacctg
    22861 gtcgccggtc aggtcctcct ggccccgttg gacttcatgg tgcgcagcca gtacctgcac
    22921 gcccgctcca ccctggagcg gttgctggag ctgggggtgg tgccggtcgt caacgagaac
    22981 gacgcgaccg cggacgacga gatccgattc ggcgacaacg accgcatcgc cgcactcgtc
    23041 gcccagctcg tcggcgccga actgctcgtc ctgctcaccg acacccccgg gctcatggac
    23101 gccgaccccc gcttctccgc cgaggcgtcg ctcatcgagg agatcgccga ggtcgaccac
    23161 gagctcgagg ccctggccgg gggagcgggt accgcgcggg gatcgggcgg aatggcctcc
    23221 aagctggccg cggccaagat ggcgtcgtgg tccggcgtcc gggccgtgat cgccgcggcg
    23281 ggccgctcgg gtgtgctcga cgacgccatc gccgggcggt ccggtgtcgg cacggtggtc
    23341 ctgccccggg cccggcggct gtcggcccgc aagctctgga tcgccttcgc cctgccctct
    23401 cggggccgca tcgtgatcga cgacggcgcg gtgcgcgccg tcgccgccgg gggccggtcc
    23461 ctgctggccg ccggtgtgac cggcgtccac ggcgacttcg acgccgacga cgcggtcgag
    23521 gtggtcggcg ccgacggcac ggtcgtggcc aagggcctgg tgcgcctggg tgcgaccacg
    23581 ctgcggtcgc gggcggggcg gcgttccgcg gacctggtcg acggggcggg cgaggtcgtc
    23641 cacgccgacg acctggtcgt gctgcccgcc tgatgcggtc ctgatcagct ggcgtcgtcc
    23701 ggagcgacgt cgcgacgacg gcgtgggcgg tggtcgtcgt cgatgatcca ggccccccgg
    23761 tcgacgtcgc ccgcctcgac ggcgcgtccg cggttgtcga cgaggagcag cccctcctcg
    23821 ggggcgtcgg cccagcgcac ccagtggccg tccaggacga ccagggcgct gccgtcgcgg
    23881 cagaggacct ggaagtcgag gatgtccggc cgaggtcggc cgtcgtagag gagactccgg
    23941 gtgcgcatgc tgcggggctg gtcggcgagg ccggcgtctt cgtcggcgtc gagttccctc
    24001 gcgagctcgt gcgcggtgtc gtcgtcgacg gcgacgtgga gcgtcacggg gtcgccgatg
    24061 atgtacaggt cgaggcgctt gccccagcac ttgatctggc cgtcgcggat cgcgtcggtg
    24121 aactcgtcgt acattcccac gagtacaggg tgccgtaacg tcagcggttc gtcgcccggg
    24181 ttcggtcgat tcgtcagggc actgtcacac ccctccggca tgatgcgaac atgcgttcga
    24241 ctggggaagg gacatcagga ggagggtcgg ccggggacgg tgctggccgg gaggcggtgt
    24301 cggcggggtt cggggtgccg g
//
LOCUS       DAOQOD010000025        24214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_703_length_24214_cov_10.398816, whole genome shotgun sequence.
ACCESSION   DAOQOD010000025 DAOQOD010000000
VERSION     DAOQOD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 24214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24214
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_24214_cov_10.398816"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLZ72_04315"
     CDS             <1..511
                     /locus_tag="PLZ72_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNA35557.1"
                     /translation="DRLPRAGRRAGSSGRPRRTPVRLARGAIDGTAEATDQLLRTPGV
                     AVVVDGYNVSMSAWPRLDARTQRDHLVKLLGAASARTGADVHVVFDGDSATEAERRPS
                     VSAPLAVRVHYSAADVEADDVVIAMARDLPTDRPVVVVSSDRRVAEGARRHGANVVSS
                     SALLAWVRA"
     gene            complement(474..>1270)
                     /locus_tag="PLZ72_04320"
     CDS             complement(474..>1270)
                     /locus_tag="PLZ72_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35558.1"
                     /translation="VTPAPLVPTADEVTSADDVATEPPEAATDAPHATTDPSTDPTTD
                     AAARARAAAGVPEGALVVGHGHVLLRDHATVDVLCAVLFAGLGSTRRSGSDRAAAWVT
                     ASATPPSADPARFPPGVRLRGRTAKVAELLENRDGGRVWVTMQPDVDIARGLARTPEL
                     PPTLAGLVTWWRAVQDLPAVITPDHAADVAAAIDAIRPRTDDTAFGPVVGQLHDALLT
                     AASDREPLVVAIGDGTTSDTSAGTYAERSAPERTVMPAPRRAAPTS"
     assembly_gap    1271..1370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1623..2219)
                     /locus_tag="PLZ72_04325"
     CDS             complement(1623..2219)
                     /locus_tag="PLZ72_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNA35559.1"
                     /translation="MNPHFWWYVSRSSGMVAAVLLVMTIVWGLLASTKVLRRPGLPAW
                     IADLHRGLGALTVVFVVVHLTALVGDNTVHFAWTELFVPFASTWRRGPVAWGIAAFWA
                     LVVVQGSSLLQRRIGRPTWRRLHYLSYPLAVLTAVHASTAGSDASSPVFRLVFLMSII
                     AMTALTLVRVIRSTLLPPRPRSARPVVAARGRTASHRS"
     gene            complement(2216..3175)
                     /locus_tag="PLZ72_04330"
     CDS             complement(2216..3175)
                     /locus_tag="PLZ72_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNA35560.1"
                     /translation="MSEHHGISEHRCHVMGTDAHIVVHGGDRSTLDRAVERLVSLEAR
                     WSRFRPESELSRLNAEAATAVVVSSDTARLVATAVAAWSLTGGRFDPTTGPALIAAGY
                     DRSFELLPVTDATGIGAAVAPIGAHPAPGCAGVVVAADVGVVHLPVGVTVDPGGIGKG
                     LAADLVAGELLESGATGVLVSLGGDLRAAGVAPGGAWDVQLDHGTGESGRIGVVEGAV
                     ATSSVLRRRWGSPTGPAHHVIDPRTGKPTTGAAVACSVIAGEAWWAEALATAVLVAFE
                     DHDGVRTAETLLGSDAAAIVTTADGATMTLGSHAIVDSMEVAA"
     gene            complement(3188..3433)
                     /locus_tag="PLZ72_04335"
     CDS             complement(3188..3433)
                     /locus_tag="PLZ72_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35561.1"
                     /translation="MAIAGAASTATFAIVAVLGGAASSDAGPAASTVNVQQRVAPPVA
                     ATAVPPGVAVDDGVVVRPVAPVTVPPRREVVVRSRAS"
     gene            complement(3489..3905)
                     /locus_tag="PLZ72_04340"
     CDS             complement(3489..3905)
                     /locus_tag="PLZ72_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35562.1"
                     /translation="MDESPNDPTTSGHPHPTRAGDGKRRRAGVAALAITVTLVAATAA
                     VAVNMSATSPPPATNVGRLTAVDDLLPATDGVTYVDQSGSVVPSPDTAPYQASRSHDD
                     DPHDDDRSHDDDRSHDSSKDGKSHDDDRHEGRDDDD"
     gene            4023..4664
                     /locus_tag="PLZ72_04345"
     CDS             4023..4664
                     /locus_tag="PLZ72_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35563.1"
                     /translation="MEDDAAIADSLVVGLGRHGFDVSVAQDGATALRLVDRCDLVLLD
                     LGLPDLDGSEVCRRVRETSGVPIIVVSARSGELDRVVLLEMGADDYVVKPFGLRELVA
                     RIRAVSRRSGDDSSTQRLDLGALVVDLRTHEVTVDGAVVDLTPKEFDLLAHLARDPGA
                     ALNRRDILEEVWDVNWYGSPKTLDVHVASLRRKIGHPEWIQAVRGVGFKLSVP"
     gene            4661..6037
                     /locus_tag="PLZ72_04350"
     CDS             4661..6037
                     /locus_tag="PLZ72_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35564.1"
                     /translation="MTRRLVAASVVITAFIMLTVEVPLGLSYTGRAEDRLLAGLERDA
                     RVFAGLVEERIEAGDHAAVIRAARRYASRTDARVVVTDAAGISLVDTAGTDARDFSTR
                     PEIDTALSGAQATGIRTSTTLGREIAYASVPISSGARITGVVRVSTSTDEMRSQIRWN
                     WARLALLLVLMLAAAAAFGSTVAHWAMAPVSQLEAGAQRLAAGDLTNRTRVDRGPPEL
                     KRLSATFDEMAARLEMLVGSQRRFVADVSHQLRTPLTVLRLRVESMDAELDAPVDADG
                     LRRDVDAVRGEIERLIRVVEGLLALTRADGSASSATVDVAAAARAARERWDALAEERS
                     VVIGIDAPRSATASEVPGGIDQILDNLLDNAIDVAPEGTAIDITVTARPNEVLVSVRD
                     RGPGLDEEARQRATERFWRGPDAAPGGTGLGLAIVAELARVSGGSMALRDPADGPGLL
                     VEVRLPST"
     gene            complement(6295..7074)
                     /locus_tag="PLZ72_04355"
     CDS             complement(6295..7074)
                     /locus_tag="PLZ72_04355"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNA35565.1"
                     /translation="MVDDTGSDSKATTRRVVIAVLVLLVAIGAVAIWLVRRGGDDSSA
                     TTTTSVATTDTSAHPTEPGSSTSTSSATAPADDATATAVWPRSDTARRFEDPVAAARS
                     FAVDFAVFADPVLGEFRQGDSRSGEVEVRPQADGPVTTVFVRQLGDDGSWWVLGSATA
                     NIEVTEPAALATIGDPVTLKGTANAFEGTVDVDIRADGRDEPLVKGFVTGRMGEMGPF
                     DSTLSFPSPHGGYGAVVFRTLSAEDGSVWEAAVVRVKFADG"
     gene            7204..7500
                     /locus_tag="PLZ72_04360"
     CDS             7204..7500
                     /locus_tag="PLZ72_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35566.1"
                     /translation="MTPVDDPEPLQARPPHVRPPHVRPLDARTAENRRVLRIDCAECA
                     HRDSPVCADCLVTFLCEHEDDGAVVVPISEIRAVRMLQAAGLAPQIRHRSTRVS"
     gene            7558..8724
                     /gene="queG"
                     /locus_tag="PLZ72_04365"
     CDS             7558..8724
                     /gene="queG"
                     /locus_tag="PLZ72_04365"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNA35567.1"
                     /translation="MGEMGLREVIVARGHANGLDAVGICEASPFTGARQDLEQRRAAG
                     LNADMEFTYRNPERSTDPSRTLAGARSLVVGAVRYASRSSDYDPPAGAVPVGSGPSGR
                     VARYAVGDQDQVLRRALDAVASDLRHAGFRTVVLSDDNALVDRAAAHRAGIGWFGKSA
                     NLLLPGRGSWFVLGAVLTDADLPTSSGPVADGCGTCRRCLDRCPTRAIVADGVVDARR
                     CLSWLLQSPGVFPHEFRDALGDRIYGCDDCQEVCPPNRLADRRHDGPRPGVAAGTGSV
                     DLCWMLEADDDELMAATRRWYVPGRDPDHLRRNALVVLGNTADGGDDAAVGLLRRYLR
                     HRDDNLVAHAAWAALRIGRSDLLDDPEVRTRPPVLDERGRWHAALAADPIRETG"
     gene            8732..9295
                     /locus_tag="PLZ72_04370"
     CDS             8732..9295
                     /locus_tag="PLZ72_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35568.1"
                     /translation="MTPLERPQSAAEARAEAEARHAEQRAERRAAFDAAYGPTAPGRP
                     VVVVSWVITAAFALAAAAALLWPERFDAAFLILSLVLFAAGCALFLVDLVLAANRSRS
                     DEMGIGGLFFLVGSAPPDVQRNLNGSLGAQVAVALAAAAVGFVRLGDRDLNAAAFGIL
                     VPTIALGLNGLWGVRWGLFPARADDAT"
     gene            9292..10476
                     /locus_tag="PLZ72_04375"
     CDS             9292..10476
                     /locus_tag="PLZ72_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNA35569.1"
                     /translation="MRVLLVTGKGGVGKTTTAAATALRLARDGHRVVVTSADPAHSLA
                     DSFDVDLGSAPTEVAPRCSAQQVDARQRFEDHWAEIRDWLLQVFDWAGVESVEAEELA
                     VLPGLDELFALTEIESLCASVDTDVVVVDCGPTAETIRLLSLPDVLGWYMDRLFPASR
                     RLHRVVAPVLSRVGRLPMADDAVFAAGQRLYDRLDAVHRILADPRVTSARLVVNPERM
                     VVAEARRTFTQLSLFGYQVDAVIVNRVLPDGVADPWFDRWRATHAEHLDTVRGDFAPV
                     PVLCSTLAPSDVVGVAALTALGDELWSAHDPHDRLVERRPLRLERLGGDDYELSIELP
                     FVDRSEVEVSRSGDELIVTLGAQRRALSLPESLRRREVTSAAVADGALRVRFCERERM
                     GA"
     gene            10473..10925
                     /locus_tag="PLZ72_04380"
     CDS             10473..10925
                     /locus_tag="PLZ72_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35570.1"
                     /translation="MNHAEDRRRDGDIDQDPGAEGVEHLQRAAREMVAAARSFLDAIE
                     KVVEDRDALREVSASLTGMAASVGDALGDAVRGRSAPWVDSASGRDPSPDRRSTATHD
                     DGTDAASEQGDPDGVVADRVDDTEDWARALVDPQAPRRPSRVRRISVE"
     gene            11038..13326
                     /locus_tag="PLZ72_04385"
     CDS             11038..13326
                     /locus_tag="PLZ72_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35571.1"
                     /translation="MRLLRLDHLPGGRSVELHPRLTVLLSAPADVRAELARILTAVVS
                     GTPVDADGQVELHGVIVGLRNRAFDVGSAARVDPVLDLAAPRPLRASVTPVPPRPPGA
                     ESPPPGPESPARGPESPPAGPGPSDGRPVVEIRSIEDHLGPEPPDPARRTRQIATDEL
                     VRLRAELRSLDGERAILARSADEVRGDLDSFARATLDVALGQLEAVESRRRSAVEERA
                     AWDAENEERRAELVSTVARLREALAAVPPADLDPLRRALDDLRSAREPSRRPDPTCRG
                     LAVELEDLRRSMEALETRRLAVEETLGAALEQLAEAEAELELARDSIRSPELHPAVVR
                     QLEAVRDEIFELEERGGRVAAVRHRRRIDELRSEEALLLDQLGFDTYTAFVMGRPNRE
                     TESVRAIREDRARSRLDQLTEEVDRLRQELPGGTEDVWNRDERDRIVDAAAEHLGTSA
                     DGLRRLTTAELIELLQSQVERPSPTASAEILTASGRVAAALVAAGAPAPAAVADPEVM
                     SDLAEAWLADEPARNARREAVTVELEQAERELEAHEGARSADDGGRLADLDAELRALQ
                     ARVAAGEEAVTRHERATAELAELRARELELRDRERDLLVRISDRERLLEVLGSDAPPD
                     PTSLPPQMPDLPPVSGAGSWVGPPPEVVAGVVAEEGRPDDTAEALQRVVQRDASVLWP
                     IDREWQLLARLGEVRSLGTVGSVPLLVHGIDAASVETPALLHRIASMSELVQMIVLSD
                     DERLGRWCDGLDVDARLLRWSS"
     gene            13366..14430
                     /locus_tag="PLZ72_04390"
     CDS             13366..14430
                     /locus_tag="PLZ72_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA35572.1"
                     /translation="MDTHVRRSPTDAEPSRRTVGIDVGGTKTLGLLVEHGPAGVAVLD
                     RERVPSRADDPSAVDAVVGVARTLTERSRNRAGEAVPVPAPVTAVGVGLAGFVDRDGV
                     VRAAPNSVGLVGQDVRGRLERELGLPVTVDNDANCAAVAAHALIEPTVADLVAVTLGT
                     GIGGGLIVDGRLVRGARGFAGEPGHMVVDPDGPRCPCGQRGCWERYASGSGLGWLARR
                     ALRRGDAPSLAEAVADPEELRGEHVTELLGVGNRDAVRVFDEYARWVALGVANLISLL
                     DPDVVALGGGVAAVGDELTGRVRTILSERFPAASDGRSVSIVTTPGGPDAGAIGAAIL
                     ARDAAAAEPEPGSGRSGDRR"
     gene            14442..15617
                     /locus_tag="PLZ72_04395"
     CDS             14442..15617
                     /locus_tag="PLZ72_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA35573.1"
                     /translation="MEFRRITDLPPYVFTIIDSLKVAARREGADIIDLGFGNPDLPSP
                     ATAVEKLCEAARNERNHRYSSSRGIPKLREAAANYYRHRFGVEIDPDTEVISTIGAKE
                     GFSHLMWVLLEKGDVALVPSPSYPIHIFGPLFAGANVREIPLGTGDEFFANMVEQWEY
                     SHPKPRVIVLSFPHNPTTTCVDLDFMQRVVDFAREHEVLLVHDNAYADLGFDGYRPPS
                     ILQADGAKDVAVELYSMTKSFSMAGWRMAFLLGNPQVIAALAKLKSYLDYGTFQPIQI
                     AATVTLNEEIDFPVEVNEIYQSRRDALCGGLQRIGWEIDPPMGTMFAWAPIPEPYREM
                     GSVEFCSHLVREAQVALSPGVGFGPGGEGHVRFALIENEQRTNQAIRNLKRALTKLG"
     gene            15699..16295
                     /locus_tag="PLZ72_04400"
     CDS             15699..16295
                     /locus_tag="PLZ72_04400"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35574.1"
                     /translation="MSERRLTPRGAERRAQLIAYATERFAADGYHPTSVADIVDGLGV
                     GKGVFYWYFSSKEELFVEILRTAQKELRRSQLRAIEDHGDPVRRLELGIRAGVLWMAE
                     HNRERKLFEFARTEPAFAGAVMAGQRTLVADAVAHLEQAIEQGLIPDRDPEALGWGIL
                     GVSNVMTRIFIDERGVEPGEVADRVVAFCMEGIGGHRP"
     gene            complement(16302..17891)
                     /gene="glpK"
                     /locus_tag="PLZ72_04405"
     CDS             complement(16302..17891)
                     /gene="glpK"
                     /locus_tag="PLZ72_04405"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNA35575.1"
                     /translation="MAVISIDAGTTGIRAFVVDRGGAPVARAYREFAQHFPRPGWIEH
                     DAEEIWSTTVTVLAEAAGLLAELLPGEAVDAIGITNQRETAVAWDARTGLPRHRAVVW
                     QDRRTADRCEQLESDGHLPRIRALTGLVLDPYFSASKFEWLLTDGGVAADDDLRLGTV
                     DSWLLWKLTSGTASAGAVHATDPSNASRTMLYDISDRRWSSELCELFGVPIGALPEVR
                     PSCGVFGLTAADGPLGGGIPVSGIAGDQQAALFGQACLSPGMAKNTYGTGSFVLLNVG
                     AECPAPVDGLLTTVAWEIPTRVFDDVAGPGGADGSDGSVGSDGSATVTHYALEGAIFV
                     TGAAVQWLRDGLGIIAEASEVGPLAASVDDTGGVFVVPAFAGLGAPDWDPYARGTVVG
                     ITRGTTRAHIARAVVEAMAFQTRDVVRSMTDATGVDLAELRVDGGAAAMDLLLQVQAD
                     QLGVPVVRSAVAETTALGAALLAGLGAGVWSSAAELAANWRADARVPPSTDPERLTAA
                     DAGHRMWRRAVTRSLAWADGG"
     gene            17944..18978
                     /locus_tag="PLZ72_04410"
     CDS             17944..18978
                     /locus_tag="PLZ72_04410"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNA35576.1"
                     /translation="MRIGMLTSGGDCPGLNAVIRAVVRKVERVHGGEVLGFRNAWSGV
                     LDDDWEPLDVQRCRGILPRGGTILGTSRVQPYQFPDGVDRVRAAMEHHRLDGFVVIGG
                     DGSLGAARDLHADGVSCIGVPKTIDNDIAATEMTFGFDTAVHVCTAAIDRLHTTAEAH
                     DRVMVVETMGRHAGHIAVRAGIAGGATMTLIPEVPFDIGEVCDALQRRHAGVSYASIV
                     VVAEGAVPVPGTMPEPEYEVDRFGHRRLGGISQRLAEEIEGRTGIETRVTILGHVQRG
                     GTPTAFDRLLATRYGVAAADAAADGAWGQMVALQADRIVRVPLAEATGGTKPVDLDLY
                     EQVARPFFAS"
     gene            complement(19030..19704)
                     /locus_tag="PLZ72_04415"
     CDS             complement(19030..19704)
                     /locus_tag="PLZ72_04415"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNA35577.1"
                     /translation="MNGSPGRRRRRIVARSLAAAVVPVAIAAGCSISTDDTPRGLAVS
                     TTTTSAPASPTSGGASAQLWYVLDGSLVPVSASLPDHLLSTVMGSLFDAPDTESVGRD
                     LTTSVPVDTKVEHLDLADGTLTVDLSAEFDNLVGPSRQQAIAQIVLTATEFPSVERVR
                     FRVDGTAVPVPTPTRGDAPTVSECDYASLLADPVEMAQAGVTLNAIQRTQARAADLER
                     HCDNTG"
     gene            complement(19701..21302)
                     /locus_tag="PLZ72_04420"
     CDS             complement(19701..21302)
                     /locus_tag="PLZ72_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA35578.1"
                     /translation="MTDAASAPADARLRRGIHPGLRARIAISVALVTLLASVAVSITT
                     LTVTRRTLIDQREESVSRRVLANARTVEGSVGRAPTQDVQSVLSSLPDAGKPSVILPG
                     DGRTVVASLDTRFGVDSIPRALRDQVAAGDSGIMRVVVDGQPVVAIGVPLSQTGGSYF
                     EVYRLDDIDAGLRTLGFTLVLATALSTVAAGAIGYWASRYTLRPLTRIGEATEAVALG
                     QLDTRLDYDEYVHDSDLAPLVTNFNSMVASLQERIDRDARFASDVSHELRSPLTTINA
                     AIQVLSNNQDQMPERSQQALELLGKDMARFTQLVEDLLEISRFDAGAVRLELDGVAIV
                     PWVRTTVSNLTDGTVEVHSDPRLDGLVLACDKRRVTRILANYLDNAEKYADGATGVFI
                     DRHDPGPDEIAEGVDPDELTVRIAVEDRGAGVPESERARIFDRFNRGDQGGSRGSDLG
                     VGLGLALVAEHARLQGGQVWVEDREDGGQGARFVVELPLLAVDEVEEDLSEATPEAAG
                     ALTLTGEHRAIGLDALPDDPPRVVS"
     gene            complement(21299..21979)
                     /locus_tag="PLZ72_04425"
     CDS             complement(21299..21979)
                     /locus_tag="PLZ72_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35579.1"
                     /translation="MGTRILTVEDDERIRTSVKLALEDEGWQVLEAETGEDAIATFTR
                     EPTDVVLIDIMLPGIDGFEVCRSVRRLSDVPIIMVTARADTHDVVAGLEAGADDYLTK
                     PFVPKELSARIRALLRRARPTVPGNPRLRFGDLEVVPQEGVVTRADQPVHLTKTEFRL
                     LVELANHPGTVFSREDLLEKVWGHGVFGDGRLVDVHVRRLRTKIEADPADPRHVVTVR
                     GLGYKLQP"
     gene            complement(22049..22864)
                     /locus_tag="PLZ72_04430"
     CDS             complement(22049..22864)
                     /locus_tag="PLZ72_04430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA35580.1"
                     /translation="MSADSADLLSLARRAADAAVATVRERPSALEVDTKSSSTDMVTQ
                     MDRAAEETIRAVILADRPDDRIVGEEMGDRPPPTGADGADAVTWWIDPIDGTTNYVYD
                     HPGWNVSIAAELGGRTVAAVVADPTHGRTYSAVRGGGAEVSDRPGLLRLVDPPPLSHA
                     LVATGFSYDPARRHRQGLVVAELLPRVRDIRRMGAAALDLCSVAAGRVDVYFEVGLSP
                     WDIAAGSLVAEEAGARVAAIDGGPLGTGSVLAAHPALFDEVIGLLDELGAASV"
     gene            complement(22861..23178)
                     /locus_tag="PLZ72_04435"
     CDS             complement(22861..23178)
                     /locus_tag="PLZ72_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35581.1"
                     /translation="MADRSPTPPPGTDPESALPSVLARALAFGAILVGGAAGGLIGYA
                     FADLGGFGGAAVGLVTFLGAVVGAGGVAVVAVLTLRALGEWETIRRRDDATRRGPGRS
                     RSA"
     gene            23274..24044
                     /locus_tag="PLZ72_04440"
     CDS             23274..24044
                     /locus_tag="PLZ72_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35582.1"
                     /translation="MELLVPPRRLGRLLSEARLEKGLTVAEVSEEMGGSLDEIDLLEI
                     ETGRRAISESDLKTLSELYGVRTSSMVPSRSQLVIDLDEGLITADGSRAELPDHDTAA
                     GREEVLSKYLALVYSMRGQEPGTTVTLRLGDLDVLAEALDSDRRTVEDELLSLMVARP
                     EPVKKRFRLLRGRTVVPVVGVLVASTTAGALVFDVDDSSAATPTPGVEEPAALAPSAE
                     LPRVIIDDAAFQERMEDGSPGPVQVRLGDASDSTPAGD"
BASE COUNT         3233 a         8637 c         8892 g         3352 t
ORIGIN      
        1 cgatcgactg ccgcgggctg gccgtcgcgc cggctcgagc gggcggcccc gccgcacccc
       61 ggtgcgactg gccagaggtg cgatcgacgg caccgccgag gccaccgatc agctgctgcg
      121 gacccccggc gtggcggtcg tcgtcgacgg ctacaacgtc tccatgtcgg cctggccacg
      181 actcgacgcc cgtacccagc gggaccacct ggtcaagctg ctcggcgcgg cctcggctcg
      241 taccggagcg gacgtccacg tcgtcttcga cggcgacagc gccaccgagg cagagcggcg
      301 tccctcggtg agcgcgccgc tggcggtgcg ggtccactac agcgcggcgg acgtcgaggc
      361 cgacgacgtg gtgatcgcca tggctcggga cctgccgacg gaccgtccgg tggtggtggt
      421 gagctcggac cggcgggtgg ccgagggggc ccgtcgccac ggggccaacg tcgtcagctc
      481 gtcggcgctg ctcgcctggg tgcgggcatg accgtccgct ccggagcgct gcgttcggcg
      541 taggtcccgg cgctggtgtc gctggtcgtc ccgtccccga tggcgacgac cagcggttcc
      601 cgatcggacg ccgcggtcag gagcgcgtcg tggagctggc cgaccaccgg accgaaggcc
      661 gtgtcgtcgg tgcggggccg gatcgcgtcg atcgcggccg cgacgtcggc ggcgtgatcg
      721 ggggtgatca ccgccggcag gtcctggacc gccctccacc acgtgaccag acccgccagg
      781 gtcgggggca gctccggcgt gcgggccagg ccgcgggcga tgtcgacgtc gggttgcatc
      841 gtcacccaga cgcgacctcc gtctcggttc tcgaggagct ctgcgacctt ggccgtccgt
      901 ccgcggagcc tgactccggg cgggaagcgg gccggatcgg ccgacggggg agtggccgag
      961 gcggtgaccc aggcggcggc tcggtccgat cccgaccgcc gggtggatcc gaggccggcg
     1021 aacagcacgg cgcacagcac gtcgacggtg gcgtggtccc ggagcaggac atgaccgtgg
     1081 ccgaccacga gggcgccctc ggggacgccg gccgcggccc gggcccgcgc cgcggcgtcg
     1141 gtggtggggt cggtgctggg gtcggtggtg gcgtggggcg cgtcggttgc tgcctcggga
     1201 ggttcggtcg cgacgtcatc agcgctcgtg acctcatcag cggtcgggac cagcggcgcg
     1261 ggcgtgacca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcatcagcgc
     1381 tcgtgacctc atcagcggtc gggaccagcg gcgcgggcgt gaccagatgc cgccagttct
     1441 cgacgaccag ggtggcgagc accgtcgctc ccatcaccgc gagcagggcg aggatggcga
     1501 ggtacgggtt cacgtcggtc ccatcggctg ttcggcggcg tcgatgagcc tcgcccggaa
     1561 cgtcgcgtcc ctctgccggg tcctccctgc tccagctcgg gtgatcccgc ggctcgggat
     1621 cctcaggacc tgtgagaagc ggtgcgtccc cgagcggcca ccaccggtcg agcgctccgt
     1681 ggccggggtg gcaggagggt cgagcggatg acacgcacca gtgtcaacgc ggtcatggcg
     1741 atgatcgaca tcaggaacac gagtcggaag accgggctcg acgcatcgct gccggcggtc
     1801 gacgcgtgca ctgccgtcag caccgccaac gggtaggaga gatagtggag ccgccgccac
     1861 gtcgggcgcc cgatgcgccg ctgcagcagc gatgatccct ggacgaccac gagcgcccag
     1921 aacgctgcga tcccccaggc gaccggtccg cgtcgccatg tgctcgcgaa cgggacgaac
     1981 agctcggtcc acgcgaagtg gacggtgttg tcgccgacga gtgcggtgag gtggaccacc
     2041 acgaacacca cggtgagcgc cccgagtcca cggtgcaggt ccgcgatcca cgccggcagg
     2101 cccgggcggc ggagcacctt ggtcgacgcc agcagccccc acacgatcgt catcacgagg
     2161 agcacggcgg cgaccatgcc gctcgaccgg gacacgtacc accagaagtg cggattcacg
     2221 ccgctacctc catcgagtcg acgatggcgt gcgagccgag ggtcatggtc gccccgtccg
     2281 cggtcgtgac gatcgcggcg gcgtcggagc ccaggagcgt ctcggcggtt cgaacaccgt
     2341 cgtgatcctc gaacgcgacg agcaccgctg tcgcgagcgc ctcggcccac caggcctccc
     2401 cggcgatgac gctgcacgcg accgctgcac cggtggtcgg cttcccggtc cgcggatcga
     2461 tcacgtggtg agcaggtccc gtcggggatc cccatctccg tcggaggacc gacgaggtgg
     2521 ccacggcacc ctcgaccaca ccgattcgac cgctctcgcc cgttccgtgg tcgagctgca
     2581 cgtcccacgc gccaccgggg gcgaccccgg ctgcacgcag gtcgccgccg agggacacca
     2641 gcacccccgt cgcacccgac tcgaggagtt caccggccac gaggtcggcc gccagcccct
     2701 tgccgatccc gccgggatcg accgtcacgc ccaccgggag gtggacgacg ccgacatccg
     2761 cagcgaccac cacccctgca cagccgggcg ctgggtgcgc cccgatcggc gccacggccg
     2821 ccccgatccc ggtcgcatcc gtcaccggga ggagctcgaa ggaacggtcg tatcccgctg
     2881 cgatcaacgc cggtcccgtc gtcgggtcga acctgccgcc ggtgagcgac cacgccgcga
     2941 ccgcggtcgc gacgagccgg gccgtgtccg agctgacgac caccgccgtt gctgcctcgg
     3001 cgttcagtcg ggacagctcg gactccggac ggaaccgcga ccaccgggct tcgaggctga
     3061 cgaggcgttc gaccgcccgg tccagcgtcg accggtcgcc gccgtggacg acgatgtggg
     3121 cgtcggtgcc catgacatgg cagcggtgct cggagatgcc gtggtgctca gacacgccca
     3181 cgtcgcttca gctcgcccgg gaacggacga cgacctcacg gcgcgggggc accgtcacgg
     3241 gagcgaccgg ccgcacgacc acaccgtcgt cgaccgccac cccgggcggc actgccgtgg
     3301 cggccacggg tggagcgacc cgctgttgga cgttcaccgt cgatgcagcg ggtccggcgt
     3361 ccgacgacgc ggcgccgccc agcacggcga cgatcgcgaa ggtcgcggtc gacgcggcac
     3421 cggcgatcgc cacccgtgac gctgccgcgg gatgccgtcg cctcctgcga accaccgggc
     3481 tctgtcggtc agtcatcgtc gtcccgtccc tcgtgccggt cgtcatcgtg ggacttgccg
     3541 tccttcgagc tgtcgtgcga cctatcgtcg tcgtgcgacc tgtcgtcgtc gtgcggatcg
     3601 tcgtcgtgcg acctgctggc ctggtagggg gccgtgtcgg ggctcggcac gaccgaaccg
     3661 gactgatcga cgtaggtgac accgtcggtg gcgggcagga ggtcgtcgac ggcggtgagc
     3721 cgcccgacgt tcgtcgctgg tggtgggctc gtcgcgctca tgttgaccgc gaccgctgcg
     3781 gtcgccgcga cgagggtgac ggtgatcgcc agtgcggcga ccccggcccg acgtcgcttg
     3841 ccgtcgccgg cacgtgtggg atggggatga ccggaggtcg tggggtcgtt gggggattcg
     3901 tccatgtcgt cgagggtacg gagcgggtgt gaacggctct ggtgggtgaa cggtgaagat
     3961 tcggtgaagc cgctgtacgt cgccgcgacg tgtgcgacgg tgtcgtcgtg aggatcttgg
     4021 cggtggagga cgatgcggcc atcgccgact ccctcgtggt cggcctcggc cgacacggct
     4081 tcgacgtctc ggtcgcacag gacggcgcca ccgcgctgcg gctcgtcgac cggtgcgacc
     4141 tcgtcctgtt ggacctcggg ctgcccgatc tcgacgggtc cgaggtctgc cgccgggtgc
     4201 gggagacctc cggagtgccg atcatcgtgg tcagcgcccg ctccggtgag ctcgatcgtg
     4261 tcgtgctgct ggagatgggt gccgacgact acgtcgtgaa gcccttcggc cttcgtgagc
     4321 tcgtcgctcg gatccgggcg gtcagtcgac gcagcggcga cgactcctcg acccaacggc
     4381 tcgacctcgg cgcgctggtc gtcgacctcc gcacccacga ggtcaccgtc gacggcgcgg
     4441 tcgtcgatct gacgcccaag gagttcgacc tgctcgcgca tctcgcgcgg gacccgggcg
     4501 ccgcgctcaa ccgacgcgac atcctcgagg aggtctggga cgtgaactgg tacggatcac
     4561 ccaagaccct cgacgtgcac gtcgcgtcgc tccgccggaa gatcggccat cccgagtgga
     4621 tccaggccgt ccgtggggtc gggttcaagc tgagcgtccc atgacccggc gactggtcgc
     4681 ggcgtcggtc gtgatcacgg ccttcatcat gctcaccgtc gaggtgccgc tcggcctgag
     4741 ctacaccggt cgcgctgagg accgcctcct ggccggcctg gagcgggacg cccgggtgtt
     4801 cgccggtctc gtcgaggagc gcatcgaggc gggtgaccac gcggccgtca tccgtgccgc
     4861 ccgccgctac gcgtccagga ccgacgctcg cgtggtcgtc acggacgcgg ccgggatcag
     4921 cctggtcgac accgcgggga ccgatgccag ggacttctcg acccgcccgg agatcgacac
     4981 cgcgctctcg ggtgcgcagg cgaccgggat ccgcacctcc accacgctcg gtcgcgagat
     5041 cgcctacgcg tcggtgccca tctcctccgg cgccaggatc accggcgtcg tccgcgtcag
     5101 cacctcgacc gacgagatgc gatcgcagat ccgctggaac tgggcccgcc tcgcgctgct
     5161 gctcgtcctc atgctcgcgg ctgccgccgc gttcggctcg acggtcgccc actgggcgat
     5221 ggcgcccgtg tcgcagctcg aggccggcgc ccaacggctc gccgcaggcg acctgacgaa
     5281 ccgcaccagg gtcgatcggg gaccacccga gctcaagcgc ctgtcggcga cgttcgatga
     5341 gatggcggcg cgcctcgaga tgctcgtcgg ttcccagcgc aggttcgtcg cggacgtctc
     5401 ccatcagctc cgcactccgc tcaccgtcct ccggctccgc gtcgagtcga tggacgcaga
     5461 gctcgacgcc cccgtggacg ccgacggact gcgccgcgac gtcgacgccg tccgcggcga
     5521 gatcgaacga ctcatccgcg tcgtcgaggg actgctggcc ttgaccaggg cggacggctc
     5581 ggcatcgtcc gcgacggtgg acgtcgccgc agctgcccga gcggcgcggg agcgctggga
     5641 cgcgctcgcc gaggagcgat ccgtggtcat cggcatcgac gcgccgcgca gcgccacggc
     5701 atcggaggtg cccggtggga tcgaccagat cctcgacaac ctcctggaca acgcgatcga
     5761 cgtcgctccg gagggcaccg cgatcgacat cacggtcacg gcgcggccga acgaggtcct
     5821 cgtcagtgtc cgtgaccggg gaccgggact cgacgaggaa gcccgacaga gggcgaccga
     5881 gcggttctgg cgtgggcccg acgccgcgcc cggtggaacc ggcctgggac tcgcgatcgt
     5941 cgctgagctg gctcgggtct ccggtggttc gatggcgctg agggaccctg cggacggacc
     6001 cggcctgctg gtcgaggtgc gccttccttc cacctgagca ggcgtggagc acggtcatct
     6061 tcgtcatggc cagttggccc gtgctcgtga cgtcgaggat cggtccgtgc agcgccacga
     6121 tccccggact cgcgttgatc gctgctgcgg ctgcggtccg cgacacgtcg ctgcggtacg
     6181 gcgcggggtc gcggcgtcgg acgcgtagca gtgaggatca ggaccgcgag ccacatcggg
     6241 accagcaccc ggtcgtggcg cgccatcagc cgccccggcc gacgtcagcg tgcgtcagcc
     6301 gtcggcgaac ttcacgcgga cgaccgccgc ctcccacacg gaaccgtcct cggcgctcag
     6361 cgtgcggaac accacggcgc cgtacccgcc atggggtgac gggaaggaga gcgtcgagtc
     6421 gaacgggccc atctcaccca tccggccggt gacgaagccc ttcaccagcg gttcgtcacg
     6481 accgtcggcc cggatgtcga cgtccaccgt gccctcgaac gcgttcgccg tccccttcag
     6541 ggtgaccggg tcaccgatcg tcgcaagcgc tgcaggttcg gtgacctcga tgttcgccgt
     6601 cgcgctgccc agcacccacc acgagccgtc gtcgcccagc tggcgcacga agacggtggt
     6661 gaccggcccg tcggcctgcg gtcggacctc cacctcaccg gagcgggaat cgccctgtcg
     6721 gaactcgccg agaaccggat cggcgaaaac cgcgaagtcg acggcgaagc tgcgcgccgc
     6781 ggcgacgggg tcctcgaagc gccgggcggt gtccgagcgc ggccacaccg ccgtggcggt
     6841 cgcatcatct gctggggccg tcgccgacga cgtcgacgtc gacgagcccg gttcggtggg
     6901 gtgcgcggac gtgtcggtgg tggcgaccga cgtggtcgtc gtggcggacg agtcatcgcc
     6961 tccacggcgg acgagccaga tcgcgacggc gccgatcgcg acgagcagaa ccagcacggc
     7021 gatcaccacc cgccgcgtcg tcgccttcga gtcgctcccg gtgtcgtcga ccatcgcccc
     7081 agttgagcat gatgcggtga ggtggagtgg ggtggcgcac gcccgtcgtc ggcccgatcg
     7141 gatcgcgggt gtcacaccgt gtgggtacgt tgcgccggac gtcgcaccga acggaggtga
     7201 ccgatgaccc cggtcgacga tccagaaccg ctccaggccc gaccacccca cgtccgacca
     7261 ccccacgtcc gaccactcga tgcccggacc gccgagaacc ggcgggtgct gcggatcgac
     7321 tgcgccgagt gcgcccacag ggacagcccg gtgtgcgccg actgcctggt cacgttcctg
     7381 tgcgagcacg aggacgacgg tgccgtcgtg gtgccgatca gcgagatccg ggcggtccgc
     7441 atgctccagg ccgccggact ggcaccgcag atccgccacc gttccacccg ggtctcctga
     7501 ccgggcaccg ggggttgcgc gcccacggac tcccgacccc ggcggcgatg ggccagcatg
     7561 ggagagatgg gactgcgaga ggtgatcgtg gcgcgcggtc acgccaacgg cctcgacgcc
     7621 gtcggcatct gcgaggcatc gccgttcacg ggtgcgcgcc aggacctcga gcagcgccgc
     7681 gccgcgggtc tgaacgccga catggagttc acctaccgca accccgagcg ctccacagac
     7741 ccgtcgcgca ccctggccgg cgcccgatcc ctggtcgtcg gcgccgtccg ctacgccagc
     7801 cgcagctccg actacgaccc acccgccggt gccgtgccgg tcggctcggg accgtccggc
     7861 cgggtcgccc gctacgcggt cggcgaccag gaccaggtcc ttcgccgtgc cctcgacgcc
     7921 gtcgcctcgg acctgcgcca cgccgggttc cgcaccgtgg tgctctccga tgacaacgcc
     7981 ctggtcgacc gggccgccgc tcaccgggcc gggatcggct ggttcggcaa gagcgccaac
     8041 ctgctgctgc cgggtcgggg gagctggttc gtgctcggcg cggtgctgac cgacgcggat
     8101 ctccccacgt cgtccggccc ggtcgccgac ggatgcggga cctgccgacg gtgcctcgac
     8161 cgctgcccga cccgggcgat cgtcgccgac ggcgtggtcg acgcccgccg gtgcctgtcc
     8221 tggttgctgc agtcgcccgg ggtgttcccc cacgagttcc gcgacgccct gggcgaccgg
     8281 atctacggct gcgacgactg ccaggaggtg tgtccgccca accggctcgc cgaccggcgc
     8341 cacgacggtc cccgacccgg cgtggcggca gggaccggga gcgtggacct ctgctggatg
     8401 ctcgaagccg acgacgacga actgatggcc gcgacccgtc gctggtacgt gccgggtcgc
     8461 gaccccgacc acctgcgtcg caacgccttg gtggtgctcg gcaacaccgc ggacggcggc
     8521 gacgacgcgg cggtggggct gctgcgacgc tacctgcgcc accgcgacga caacctggtg
     8581 gcccacgcgg cctgggcggc cctgaggatc ggccgctccg acctgctcga cgacccggag
     8641 gtccggacgc gtcccccggt gctcgacgag cgcggtcggt ggcacgcggc actggccgcg
     8701 gatccgatcc gcgagacagg atgaccggac gatgaccccg ctcgagcgtc cgcagagcgc
     8761 cgccgaggcg cgcgccgaag ccgaggcccg ccacgccgag cagcgagccg agcgtcgagc
     8821 cgccttcgac gccgcctacg gcccgaccgc gcccggtcgg cccgtcgtgg tcgtgtcgtg
     8881 ggtcatcacc gccgcgttcg ctctggcggc ggccgcggcg ttgctgtggc ccgagcgctt
     8941 cgacgctgcg ttcctcatcc tgtcgttggt gctgttcgcg gcggggtgcg ctctgttcct
     9001 ggtcgacctc gtcctggccg ccaaccggag ccggtccgac gagatgggca tcggtggcct
     9061 gttcttcctg gtcggatccg ctccgccgga cgtgcagcgg aacctgaacg gctccctcgg
     9121 agcacaggtg gcggtggcgc tggccgcggc cgcggtgggc ttcgtgcgcc tgggcgaccg
     9181 cgacctcaac gccgccgcgt tcggcatcct ggtcccgacg atcgctctgg gactcaacgg
     9241 cctgtggggc gtccgctggg gcctgttccc ggctcgtgcg gacgacgcca catgagggtg
     9301 ctgctcgtca ccggcaaggg cggtgtcggc aagacgacca ccgccgcggc caccgcgctg
     9361 cgactggcac gcgacggcca ccgggtcgtc gtgacctcgg ccgatccggc ccactcgctc
     9421 gcggattcct tcgacgtcga cctggggtcg gcacccaccg aggtcgcgcc gcggtgctcg
     9481 gcccagcagg tcgacgcccg tcagcgcttc gaggaccact gggccgagat ccgtgactgg
     9541 ctgctgcagg tcttcgactg ggccggggtc gagtcggtcg aggccgagga gctggccgtc
     9601 ctgccgggtc tcgacgagct gttcgccctg accgagatcg aatcgctgtg cgcgtcggtc
     9661 gacaccgacg tggtcgtcgt cgactgcggc cccaccgccg agacgatccg gctcctgtcg
     9721 ctgcccgacg tgctcggctg gtacatggac cggctgttcc ccgcctccag acggctgcac
     9781 cgggtcgtcg ccccggtcct gtcgcgtgtc ggtcgcctgc cgatggccga cgacgccgtg
     9841 ttcgccgccg gtcagcggct ctacgaccgc ctcgacgccg tgcaccgcat cctcgcggat
     9901 cctcgggtga cgtcggcccg tctggtggtc aacccggagc ggatggtggt ggccgaggcc
     9961 cgtcggacct tcacccagct gtcgctgttc ggctaccagg tcgacgcggt catcgtgaac
    10021 cgggtgcttc ccgacggggt ggcggacccg tggttcgacc gttggcgggc cacgcacgcc
    10081 gagcacctcg acaccgtccg cggcgacttc gcccccgtcc ccgtcctgtg ctcgacgttg
    10141 gctccctccg acgtcgtcgg ggtggccgcg ctcaccgcgc tgggcgacga gctgtggtcc
    10201 gcccacgacc cccacgaccg gttggtggag cgtcgaccgc tgcggctgga gcggctgggc
    10261 ggcgacgact acgagctttc gatcgagctc ccgttcgtcg atcgctccga ggtcgaggtg
    10321 tcgcgcagcg gtgacgagct catcgtcacc ctcggggccc aacggcgggc gttgtcgttg
    10381 cccgagtcgc tccgccggcg cgaggtgacc tcggccgccg tcgccgacgg cgcgctgcgg
    10441 gtccggttct gcgaacgcga gaggatgggg gcgtgaacca cgcggaggac cgtcgtcgtg
    10501 acggcgacat cgaccaggat cccggcgccg agggggtcga gcacctccag cgagcggccc
    10561 gtgagatggt ggcggcggcc cgctcgttcc tcgatgccat cgagaaggtc gtcgaggacc
    10621 gcgatgcgct ccgtgaggtg tcggcctcgc tgacgggcat ggcggcctcc gtgggcgacg
    10681 ccctgggcga cgcggtgcgg ggccgctccg ctccgtgggt ggacagcgcg tcggggcggg
    10741 acccgtcgcc tgaccgtcgc tcgaccgcca cccacgacga cgggacggac gcagcttccg
    10801 agcagggcga tccggacggg gtcgttgccg accgggtcga cgacaccgag gactgggccc
    10861 gagcactggt cgacccgcag gcgccccgac ggccgtcgcg ggtcaggcgc atctcggtcg
    10921 agtgacgccc agcgctccgc gcgtcggtcg gggccggggg aacggccccg aaccgggtcg
    10981 gcttccggag cggaatcctc accgtcgagg tccgaccggc cgacagtggg atggaccatg
    11041 agactcctac gtcttgacca cctccccggc ggccgatcgg tcgagctgca cccgcgcctg
    11101 acggtgctgc tcagcgcgcc cgcggacgtc cgggcggagc tcgcccggat cctgacagcg
    11161 gtggtgtcgg ggacgccggt cgacgccgat ggtcaggtcg agctgcacgg ggtgatcgtc
    11221 ggcctgcgca accgcgcctt cgacgtgggt tccgcggcgc gggtggatcc ggtcctcgac
    11281 ctcgccgcgc cgaggccgct gcgggcgtcg gtcactcccg tcccgccgcg tccgcccgga
    11341 gcggaatcgc cgcctcccgg acctgagtcc ccagcccgtg gacctgagtc cccacccgcc
    11401 ggcccgggcc cgagcgacgg ccgtccggtg gtcgagatcc gctccatcga ggaccacctc
    11461 ggtcccgagc cccccgaccc cgcccgtcgc acccgccaga tcgcgaccga cgagctggtg
    11521 cggctgcgcg ccgagctccg atccctggac ggcgagaggg cgatcctggc ccgatcggcc
    11581 gatgaggtcc gtggcgacct ggacagcttc gcccgggcca ccctcgacgt ggcgctcggc
    11641 cagctcgagg cggtcgagtc acgtcggcgc tcagcggtcg aggaacgagc ggcatgggac
    11701 gccgagaacg aggaacgccg ggcggagctg gtctcgaccg tcgctcggtt gcgcgaggcc
    11761 ctcgccgccg tgccgccggc cgatctggac ccgttgcggc gggctctcga cgacctccga
    11821 tcggcgcgtg agccgtcgcg tcgccccgac ccgacgtgtc gcgggctggc ggtggagctc
    11881 gaggacctcc gtcgctcgat ggaggccctc gagacccgtc gactggcggt cgaggagacg
    11941 ctgggcgccg ccctcgagca gctggccgag gccgaggccg agctcgagct cgcccgggac
    12001 tcgatccgct ctccggagct gcaccccgcc gtcgtgcgtc agctcgaggc cgtgcgtgac
    12061 gagatcttcg agctggagga gcgcggcggt cgggtggctg cggtccgtca ccggcgtcgg
    12121 atcgacgagc tccggagcga ggaggcgctg ctgctcgacc agctcggctt cgacacgtac
    12181 accgcgttcg tcatggggcg tccgaaccgg gagaccgagt ccgtgcgggc gatccgcgag
    12241 gaccgggcga ggtcccggct ggatcaactg accgaggagg tcgaccgact ccgccaggaa
    12301 ctgccgggcg gcaccgagga cgtctggaac cgcgacgagc gcgaccggat cgtcgacgcc
    12361 gccgccgagc acctcggcac gtccgccgac ggtctgcgtc gcctgaccac cgccgagctc
    12421 atcgagctgc tgcagagcca ggtcgagcgg ccgtcgccga cggcaagcgc cgagatcctg
    12481 accgcgtcgg gccgggtcgc cgccgctctg gtcgccgcag gggccccggc gcccgccgcg
    12541 gtcgcggacc ccgaggtgat gagcgacctg gccgaggcat ggctcgccga cgagcccgct
    12601 cggaacgccc gtcgggaggc ggtgacggtg gagctcgagc aggctgagcg cgagctcgag
    12661 gcccacgaag gcgcgcggtc ggccgacgac ggcggccgcc tcgccgacct cgacgccgag
    12721 ctgcgggcgc tgcaggcccg agtggccgcc ggcgaggagg ccgtcacccg tcacgagcgc
    12781 gcgacggcag agctcgccga actgcgcgcc cgggagctcg agctgcgcga ccgggaacgt
    12841 gacctcctgg tccgcatctc cgatcgcgag cgcctcctcg aggtgctggg ctccgacgct
    12901 ccgccggatc cgacctcgct gccgccccag atgccggacc tgccgcccgt gtcgggcgcc
    12961 ggctcatggg tggggcctcc acccgaggtc gtggccgggg tcgtggccga ggagggccga
    13021 cccgacgaca cggccgaagc cctgcagcgg gtggtccaac gcgacgcatc ggttctctgg
    13081 ccgatcgacc gcgagtggca gctgctggcc cggctgggcg aggtccggtc gctggggacg
    13141 gtggggtcgg tcccgttgct cgtgcacggc atcgacgccg cctcggtcga gacgccggcg
    13201 ctcctgcacc ggatcgcgtc gatgtcggag ctggtccaga tgatcgtgct ctccgacgac
    13261 gaacgtctgg gtcggtggtg cgacgggttg gacgtcgacg cccgtcttct gcgctggtcg
    13321 tcctgagtcg cgggccgacg gctcatccgc ggccacactg tcggggtgga cacccatgtt
    13381 cggcgaagtc cgaccgatgc ggagccgtcg cgtcgcaccg tcggcatcga cgtcggcggg
    13441 accaagaccc tgggcctgct cgtcgagcac gggccggccg gagtggcggt cctggaccgc
    13501 gagcgtgtgc cgtcgcgggc ggatgacccc agcgcggtcg acgccgtcgt cggggtcgcc
    13561 cgaacgctga ccgagcggag ccggaaccgg gcgggcgagg ccgtccccgt cccagcgccc
    13621 gtcaccgcgg tcggcgtcgg cctggccggc ttcgtcgacc gtgacggggt ggtgcgggcg
    13681 gccccgaact cggtcggact cgtcggtcag gacgtgcggg ggcgactgga gcgcgagctc
    13741 ggcctgccgg tcaccgtcga caacgacgcc aactgcgccg cggtggccgc ccacgccctg
    13801 atcgagccga cggtggcgga cctggtggcg gtgaccctgg ggacggggat cggcggcggt
    13861 ctgatcgtcg acggccggct ggtccgcggc gcccgcggct tcgccggcga gccgggccac
    13921 atggtggtcg accccgacgg gccgcggtgt ccctgcggtc agcgcgggtg ctgggagcgc
    13981 tacgcgtcgg gctccggcct ggggtggctg gcccgacgcg cgctccggcg tggcgacgcg
    14041 ccgtcgttgg cggaggccgt tgcggatccc gaggagctac ggggcgagca cgtcaccgag
    14101 ctgctcggtg tcggcaaccg ggacgcggtc cgggtgttcg acgagtacgc ccgatgggtg
    14161 gcgctcggcg tggcgaacct gatcagcctc ctcgatcccg acgtcgtggc gctcggcggg
    14221 ggagtcgcgg ccgtgggcga cgagctgacc ggtcgggtgc gcacgatcct gtccgagcgg
    14281 ttcccggccg cttccgacgg tcggtccgtg tcgatcgtca ccacgccggg cggtcccgac
    14341 gccggagcga tcggagccgc gatcctcgcc cgcgacgcgg ccgcggccga gcccgaaccg
    14401 ggttccggtc ggagcgggga ccggcggtag cgtctgggcc catggagttc cgccggatca
    14461 ccgacctgcc gccgtacgtc ttcacgatca tcgactcgct gaaggtggcg gcccggcgcg
    14521 agggcgcgga catcatcgac ctggggttcg ggaaccccga cctgccgtcc ccggcgaccg
    14581 cggtcgagaa gctgtgcgag gccgcccgca acgagcgcaa ccaccgctac tcgtcgtccc
    14641 ggggcatccc caagctgcgc gaggcggccg cgaactacta ccgccaccgc ttcggcgtgg
    14701 agatcgaccc cgacaccgag gtgatctcga ccatcggcgc caaggagggc ttcagccacc
    14761 tgatgtgggt gctgctcgag aagggcgacg tcgcgctcgt gccgtcgccc agctacccga
    14821 tccacatctt cggcccgctg ttcgccgggg ccaacgtgcg tgagatcccg ctcgggaccg
    14881 gcgacgagtt cttcgccaac atggtcgagc agtgggagta ctcccacccc aagccccggg
    14941 tgatcgtgct gtcgttcccg cacaacccga cgaccacctg cgtggacctt gacttcatgc
    15001 agcgggtggt cgacttcgct cgggagcacg aggtgctgct ggtccacgac aacgcctacg
    15061 cggacctggg cttcgacggc taccggccgc cgtcgatcct gcaggccgac ggggccaagg
    15121 acgtcgccgt cgagctgtac tcgatgacca agtcgttctc gatggcgggc tggcggatgg
    15181 cgttcctgct cggcaacccg caggtgatcg cggcgctggc caagctgaag tcgtacctgg
    15241 actacgggac cttccagccg atccagatcg ccgccacggt caccctcaac gaggagatcg
    15301 acttcccggt cgaggtcaac gagatctacc agtcgcgccg cgacgcgctc tgcggcggcc
    15361 tccagcggat cggctgggag atcgatccgc cgatgggcac gatgttcgcc tgggccccca
    15421 tccccgagcc gtaccgggag atgggctcgg tcgagttctg ctcccacctg gtgcgcgagg
    15481 cgcaggtggc gctgtcgccc ggcgtcggct tcggccccgg cggcgagggc cacgtgcgct
    15541 tcgccctcat cgagaacgag cagcgcacca accaggccat ccggaacctc aagcgggcgc
    15601 tcaccaagct gggttgaacc cccgggcggc cggacccgtc ctcgaccagc acgtgccctt
    15661 gaccagtcgg tcaagttcgt gggctagcgt cggcgcccat gtcggagcga cggctgaccc
    15721 cccgaggggc ggaacgacgg gcgcagctca tcgcgtacgc caccgagcgg ttcgccgccg
    15781 atggctacca cccgacctcg gtggccgaca tcgtcgacgg cctcggcgtc ggcaagggcg
    15841 tcttctactg gtacttctcc tccaaggagg agctcttcgt cgagatcctc cgcaccgccc
    15901 agaaggagct gcgccggtcc cagctgcgtg cgatcgagga tcacggagat ccggtccgcc
    15961 gcctggagct cggcatccgg gccggggtgc tgtggatggc cgagcacaac cgggagcgca
    16021 agctgttcga gttcgcgcgc accgagccgg ccttcgccgg ggcggtcatg gccggtcagc
    16081 ggacgctggt cgccgatgcc gtcgcgcacc tggagcaggc gatcgaacag ggactgatcc
    16141 ccgaccgcga ccccgaggcg ctgggctggg ggatcctggg cgtctccaac gtgatgacgc
    16201 gcatcttcat cgacgaacgc ggcgtcgagc ccggcgaggt ggcggaccgg gtggtcgcgt
    16261 tctgcatgga gggcatcggc ggacaccggc cctgagccgc gtcagccgcc gtcggcccag
    16321 gcgagcgagc gggtcaccgc ccgtcgccac atgcggtgtc cggcgtccgc ggcggtgagg
    16381 cgctccggat ctgtcgacgg cgggacccgg gcatcggctc gccagttggc ggcgagctcg
    16441 gccgcactcg accacacccc ggcgcccagc ccggccagca gagcggcgcc gagcgcggtg
    16501 gtctcggcca ccgcggagcg caccaccggg acgccgagct gatccgcctg gacctgcagc
    16561 aggaggtcca tcgccgccgc gccgccgtcg acgcgcagct cggcgagatc gacgccggtg
    16621 gcgtcggtca tcgaccgcac cacgtcgcgg gtctggaacg ccatcgcctc cacgaccgca
    16681 cgggcgatgt gcgcccgggt cgtaccccgg gtgatgccca ccaccgtgcc ccgcgcgtac
    16741 gggtcccagt cgggcgcgcc gagccccgcg aacgccggca cgacgaacac ccctccggtg
    16801 tcgtcgaccg aggcggcgag cggccccacc tcggaggcct cggcgatgat ccccaggccg
    16861 tcgcggagcc actgcaccgc ggcgccggtc acgaagatcg ctccctcgag cgcgtagtgg
    16921 gtcaccgtcg ccgacccgtc ggagccgact gacccgtcgg acccgtcagc gccgccgggt
    16981 ccagccacgt cgtcgaagac ccgggtcggg atctcccagg cgaccgtggt gagcaggccg
    17041 tcgaccggcg ccgggcactc ggcgccgacg ttgagcagca cgaagctccc ggtcccgtag
    17101 gtgttcttgg ccatcccggg gctcaggcac gcctggccga acagcgccgc ctgctggtcc
    17161 ccggcgatgc ccgacaccgg gatgccgccg ccgaggggac cgtccgccgc ggtgagcccg
    17221 aagacgccgc acgacggccg gacctcgggc agcgctccga tcggcacccc gaacagctcg
    17281 cacagctccg acgaccaccg ccgatcggag atgtcgtaca gcatcgtgcg cgacgcgttg
    17341 gacgggtcgg tggcgtggac agctccggca gaggcggttc cgctggtgag cttccacagc
    17401 agccagctgt cgacggtgcc gagccgcagg tcgtcgtccg cggcgacgcc gccgtcggtg
    17461 agcagccact cgaacttcga cgccgagaag tacgggtcga gcaccagccc cgtcagggcc
    17521 cggatgcgcg gcaggtgccc gtcgctctcc agctgctcgc agcggtcggc ggtgcggcgg
    17581 tcctgccaca ccaccgcacg gtggcggggg agcccggtgc gggcgtccca cgcgacggcg
    17641 gtctcgcgct ggttggtgat gccgatcgcg tcgaccgctt cgcccgggag cagctccgcc
    17701 agcagtccgg cggcctcggc gaggacggtg acggtggtgg accagatctc ctcggcgtcg
    17761 tgctcgatcc agcccggtcg ggggaagtgc tgtgcgaact cccggtacgc ccgcgcgacc
    17821 ggggcgccgc cccggtcgac cacgaacgcc cggatcccgg tggtccccgc atcgatcgag
    17881 atcacagcca cggcggcgac agtagtgccc ggcgatcgcg gcggatcgcc ctacgctgaa
    17941 ccggtgcgca tcgggatgct gacctcgggc ggcgactgcc ccggcctcaa cgccgtgata
    18001 cgggcggtgg tgcgaaaggt cgaacgcgtg cacgggggtg aggtgctcgg gttccgcaac
    18061 gcctggagcg gcgtgctcga cgacgactgg gaacctctcg acgtccagcg ctgccggggg
    18121 atcctgcccc ggggcggcac catcctcggc acctcgaggg tccagccgta ccagttcccc
    18181 gacggggtcg accgggtccg cgccgcgatg gagcaccacc gactcgacgg gttcgtggtc
    18241 atcgggggcg acggctcgct cggcgcggcc cgggacctcc acgccgacgg cgtcagctgc
    18301 atcggcgtgc ccaagacgat cgacaacgac atcgccgcga ccgagatgac cttcggcttc
    18361 gacaccgcgg tccacgtgtg caccgccgcg atcgaccgcc tccacaccac cgccgaggcg
    18421 cacgaccggg tgatggtggt cgagacgatg ggccggcacg cgggccacat cgcggtccgg
    18481 gccggcatcg ccggcggggc caccatgacg ctgatccccg aggtgccctt cgacatcggc
    18541 gaggtgtgcg acgccctgca acgccgccac gccggcgtgt cgtacgcgtc gatcgtcgtg
    18601 gtcgccgaag gtgcggtccc ggtcccgggg accatgccgg agcccgagta cgaggtggac
    18661 cgcttcggtc accgtcggct cggcgggatc tcccagcgcc tggccgagga gatcgagggt
    18721 cggaccggca tcgagacccg ggtcaccatc ctcggtcacg tgcagcgcgg cggcaccccg
    18781 accgccttcg accggttgct cgccacccgc tacggcgtcg cggccgcaga tgccgcggcc
    18841 gacggagcgt ggggccagat ggtggcgctg caggccgacc ggatcgtccg ggtgccgttg
    18901 gccgaggcca ccggggggac caaaccggtc gacctggacc tctacgagca ggtcgcccgc
    18961 ccgttcttcg ccagctgatt cggccgacgg tggaccgcat cgagcgccgt tccgatcgcg
    19021 gtcgcgctgt cagccggtgt tgtcgcagtg acgttcgagg tccgcggcgc gggcctgggt
    19081 gcgctggatg gcgttgagcg tgacgccggc ctgggccatc tcgacggggt cggccagcag
    19141 cgacgcgtag tcgcactccg acacggtggg cgcgtcgccg cgggtgggcg tcggcaccgg
    19201 aaccgccgtg ccgtcgacgc ggaaccggac ccgttcgacg ctcgggaact cggtcgcggt
    19261 caggacgatc tgcgcgatcg cctgctggcg gctgggcccg accaggttgt cgaactcggc
    19321 ggacaggtcg acggtcaggg tcccgtccgc gaggtcgagg tgctcgacct tggtgtcgac
    19381 ggggaccgag gtggtcaggt cccggcccac cgactcggtg tcgggagcgt cgaacagcga
    19441 tcccatgacc gtggagagca ggtggtcggg cagcgatgcc gacaccggca ccagcgagcc
    19501 gtcgagcacg taccagagct gggcgctggc gccgccgctg gtcggcgagg ccggagcgga
    19561 ggtggtggtg gtgctgaccg ccagcccccg cggggtgtcg tcggtgctga tcgagcaccc
    19621 ggccgcgatc gcgacgggga ccacggcggc ggccagcgac cgggccacga tccgccgtcg
    19681 tcggcgaccc ggcgaaccgt tcacgacacg accctcgggg gatcgtcggg gagggcgtcg
    19741 agcccgatgg cccggtgctc cccggtgagg gtgagggcac cggcggcctc cggcgtggct
    19801 tccgacaggt cttcttcgac ctcgtccacg gcgagcaggg ggagctcgac cacgaaccgc
    19861 gctccctgtc cgccgtcctc gcggtcctcg acccagacct ggccgccctg gaggcgggcg
    19921 tgctcggcca cgagggccag accgagtccc accccgaggt ccgatccccg gctgccgccc
    19981 tggtcgccgc ggttgaaccg gtcgaagatc cgggcccgtt ccgactcggg aacacctgcg
    20041 ccgcggtcct cgaccgcgat ccgcacggtc agctcgtcgg ggtcgacccc ttcggcgatc
    20101 tcgtcgggac cggggtcgtg gcggtcgatg aacacgccgg tggccccgtc cgcgtacttc
    20161 tcggcgttgt cgaggtagtt ggccaggatg cgcgtcaccc tccgcttgtc gcacgcgagg
    20221 acgaggccgt cgaggcgggg gtcggagtgc acctcgacgg tcccgtcggt gaggttggac
    20281 acggtggtgc gcacccacgg gacgatggcg accccgtcga gctcgaggcg gaccgctccg
    20341 gcgtcgaagc gcgagatctc cagcaggtcc tcgacgagct gggtgaagcg ggccatgtcc
    20401 ttgcccagca gctcgagggc ctgctgggag cgctcgggca tctggtcctg gttgttcgac
    20461 agcacctgga tggcggcgtt gatcgtggtc aacggcgagc ggagctcgtg gctgacgtcg
    20521 ctggcgaatc gggcgtcgcg gtcgatgcgc tcctgcaggc tcgccaccat cgagttgaag
    20581 ttcgtcacca ggggggccag gtcggagtcg tggacgtact cgtcgtagtc gagccgggtg
    20641 tcgagctggc cgagggcgac cgcctcggtc gcctcgccga tgcgggtcag cggccgcagc
    20701 gtgtaccggc tggcccagta gccgatcgca cccgcggcca ccgtcgacag cgcggtggcc
    20761 agcacgagcg tgaagccgag ggtccgcaac ccggcgtcga tgtcgtcgag gcggtacacc
    20821 tcgaagtagg agccgccggt ctggctgagc ggaacgccga tggcgacgac gggctggccg
    20881 tcgacgacca cgcgcatgat gccgctgtcg cccgccgcga cctggtcgcg cagggcgcgg
    20941 gggatggagt cgaccccgaa gcgggtgtcc aggctggcca cgaccgtccg accgtcgccg
    21001 ggcaggatga ccgacggctt gccggcgtcg gggagcgacg acagcaccga ctggacgtcc
    21061 tgggtcgggg cgcgtccgac cgacccctcg acggtgcggg cgttggccag cacgcgacgc
    21121 gacaccgact cctcgcgctg gtcgatcagg gtccgtcggg tgaccgtcag cgtcgtgatc
    21181 gacacggcga ctgacgccag cagggtgacg agcgcgaccg agatggcgat gcgggcccgg
    21241 aggccggggt ggatgccacg ccgtagccgg gcgtcggctg gagccgacgc ggcgtcggtc
    21301 acggctggag cttgtagccg agcccgcgca cggtgacgac gtggcggggg tcggccgggt
    21361 cggcctcgat cttggtgcgc agccgccgga cgtggacgtc gaccagtcgg ccgtcgccga
    21421 agacgccgtg accccacacc ttctccagca ggtcctcgcg cgagaacacg gtgccggggt
    21481 ggttggccag ctcgacgagc aggcggaact cggtcttggt caggtggacc ggctggtcgg
    21541 cccgggtgac gaccccctcc tgggggacga cctcgaggtc gccgaagcgc agccgcgggt
    21601 tgccgggcac ggtcgggcgg gcccggcgca gcagggcccg gatgcgcgcc gagagctcct
    21661 tggggacgaa cggcttggtc aggtagtcgt ccgctccggc ctcgaggccg gcgaccacgt
    21721 cgtgggtgtc ggcgcgggcg gtgaccatga tgatcggcac gtcgctcagg cgacgcaccg
    21781 accggcagac ctcgaagccg tcgatgccgg ggagcatgat gtcgatcagc acgacgtcgg
    21841 tcggctcccg ggtgaacgtg gcgatggcgt cctcgccggt ctcggcctcg agcacctgcc
    21901 agccctcgtc ctccagggcg agcttcaccg aggtgcggat tcgttcgtcg tcctcgacgg
    21961 tcaggatgcg cgtgcccacg gcggccattg tccaccatcc ggagggggtg cgggtgacag
    22021 cggcccgcag cagtcgctgg agccgggctc agaccgaggc ggcgcccagc tcgtcgagca
    22081 gcccgatcac ctcgtcgaac agcgccgggt gggcggcgag gaccgagcct gtccccagcg
    22141 gtccgccgtc gatggcggcg acccgggcgc cggcctcctc ggcgacgagg gacccggcgg
    22201 cgatgtccca gggggacagc ccgacctcga agtagacgtc gacccgcccg gcggcgaccg
    22261 agcacaggtc gagagcggcg gcgcccatcc tccggatgtc gcggacgcgg gggagcagct
    22321 cggcgacgac gaggccctgg cgatggcgac gggccgggtc gtaggagaag ccggtggcga
    22381 ccagggcgtg cgagagcggg ggcggatcga ccaggcgcag cagtccgggc cggtcgctga
    22441 cctcggctcc gccgccacgg actgctgagt aggtgcggcc gtgggtcgga tccgccacga
    22501 ccgcggcgac ggtccggccg ccgagctcgg cggcgatcga gacgttccaa cccgggtggt
    22561 cgtacacgta gttggtggtg ccgtcgatcg ggtcgatcca ccaggtgacc gcgtccgcgc
    22621 cgtcggcccc ggtcggtggc gggcggtcgc ccatctcctc gcccacgatc cgatcatcgg
    22681 gtcgatcggc caggatcacg gcacggatcg tctcctcggc ggcgcggtcc atctgggtga
    22741 ccatgtcggt gctcgacgac ttggtgtcga cctcgagggc gctgggccgc tcccgcaccg
    22801 tcgccaccgc ggcgtccgcg gctcgtcggg ccaacgagag caggtcggcg gaatcagcgc
    22861 tcacgcggac cgggaccggc cgggaccgcg tcgggtggcg tcgtcgcggc ggcggatcgt
    22921 ctcccactcg cccagggcgc gcagggtcag taccgcgacc acggcgaccc caccggcccc
    22981 gacgaccgct cccaggaacg tcacgagccc gaccgcggcg ccgccgaagc ctccgaggtc
    23041 ggcgaaggcg tagccgatca gcccgccggc cgcccctccg acgaggatcg cgccgaacgc
    23101 cagggcacgg gccagcaccg acgggagcgc ggactccggg tcggtgccgg ggggcggtgt
    23161 cggcgaacgg tcggccatgg cgccgatgat acgagtgggc accggcgtgg tccgaacccc
    23221 ggcctcgatg tgaggaaagt tcaagatcgc cggccgaacg ccgatcggga accatggaac
    23281 tcctggtacc gccgcgacgc ctcggtcgcc tcctgtccga ggcccgtctg gagaagggcc
    23341 tcaccgtggc cgaggtcagc gaggagatgg gcggttcgct cgacgagatc gatctcctcg
    23401 agatcgagac cggacgccgc gccatctccg agtcggacct gaagacgctg tcggagctgt
    23461 acggcgtccg cacgtcgtcg atggtgccga gccgcagcca gctggtgatc gacctggacg
    23521 agggtctgat caccgcggac ggctcgcggg cggagctgcc cgatcacgac accgcggcgg
    23581 gccgcgagga ggtgctgagc aagtacctgg ccctcgtgta ctcgatgcgg ggccaggagc
    23641 ccggcacgac ggtcaccctc cgactcggcg acctcgacgt gctggccgag gcgctcgaca
    23701 gcgaccggcg gaccgtcgag gacgagctgc tgtcgctgat ggtcgcccgg cccgaaccgg
    23761 tcaagaagcg cttccggctg ctgcgcggtc ggaccgtggt gccggtggtc ggtgtgctcg
    23821 tcgcctcgac caccgccggc gcgctcgtgt tcgacgtcga cgactcgtcc gcagccaccc
    23881 ccacccccgg ggtcgaggaa cccgcggccc tcgccccgag cgccgagctg ccccgcgtca
    23941 tcatcgacga cgccgcgttc caggagcgga tggaggacgg gtcgcccggt ccggtgcagg
    24001 tccgactcgg tgacgcctcc gacagcaccc cggccggaga ctgatcctgg ttccgccgat
    24061 cggcggagcc gacgcctcag gcggcggtga ggaggtggtg gagtagttcg tcgagggcgt
    24121 aggcggtgag gtgttcaccg ccgccgtggc agcgacacga gtcagccccg atgtctggtg
    24181 gcggttcggg tgggtgtgcc tggaggggca gcac
//
LOCUS       DAOQOD010000026        23883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_718_length_23883_cov_11.239424, whole genome shotgun sequence.
ACCESSION   DAOQOD010000026 DAOQOD010000000
VERSION     DAOQOD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 23883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23883
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_23883_cov_11.239424"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PLZ72_04445"
     CDS             complement(<1..820)
                     /locus_tag="PLZ72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35583.1"
                     /translation="MRTLAVCCADWPVVAIGPPADRPVAVMAAHRVIAASPAARAEGV
                     VAGLRRREAQARCPSVELHDSDESRDVRAFEAVLAALEDLAPRLEVVEAGRCLVPTRG
                     PSRYHGGDRALADKVLAAVRRAVEAELDVAWPVTVGVGIADGPRAAGLVADEARRRAS
                     GAAPIVVAPGATAGFLSPLPVSRLEVLGREPAELVDVLRRLGLRTLGRFAALEAPDVL
                     ARFGARGADLHQLASGRERLPPLVSAAPVDLRVSMSLDPPADRVDRAAFAAKALA"
     gene            complement(913..1686)
                     /locus_tag="PLZ72_04450"
     CDS             complement(913..1686)
                     /locus_tag="PLZ72_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35584.1"
                     /translation="MVAGELASDGGAVPREQLRPALRAVPSITRGSIDPVRGDRSEKL
                     ARLRELAGDLSPLTLARERSLPVLSSLEELLPDGLQRGSTVAFRGAGSTTLAQAVMAG
                     PTRAGSWAACVGASTMGWAAAVEAGVELERVAVIRSDEGRAPAVLAALVDAFDLVLIG
                     PEHRPDGTEMRRLVARARERGAVLLKLADHPGRGSRVDGGGAGPGPDVELVCESSSWS
                     GPGEGWGSLLARRVTISVRGRRGFDRPRRVDLWLPSPDR"
     gene            complement(1913..2245)
                     /locus_tag="PLZ72_04455"
     CDS             complement(1913..2245)
                     /locus_tag="PLZ72_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35585.1"
                     /translation="MGQSVTVLEKPSNRPGIVRFEINRILTGMGHERYRADQPIEGDR
                     PPDVLARRLFERGGIETVSIYSNVITVDLGRGSDTDGIAEIIGDLYTYYRPGVEVPDA
                     ASFGASAD"
     gene            2343..2834
                     /locus_tag="PLZ72_04460"
     CDS             2343..2834
                     /locus_tag="PLZ72_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HNA35586.1"
                     /translation="MSTPPGPDTTDAAATTDGDDTTDGDDDGTHRVTVFEAVGGQSFF
                     DRLVDRFYERVETDDVLLSMYPEPDDLAPARRRLALFLGQYWGGPAVYAEERGHPRLR
                     MRHQPFVVGDRERRHWLAAMEDSLTETIVEAPLEPELRDAVEQRMREYFEMSAAAMVN
                     SDR"
     gene            complement(3087..3551)
                     /locus_tag="PLZ72_04465"
     CDS             complement(3087..3551)
                     /locus_tag="PLZ72_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35587.1"
                     /translation="MSGADTATSTDGTAGPARTRSVLVPVGAFVVWTLFVWIGRIRNA
                     VTDPALSGSDRIGPLVLSGIFVVGALVLGALLYRDRVMRSAASARMLRNAVRAFAGYT
                     VVIWVVRAGDIAFGGDHEAAFVVVHTVLAAVSIALAVVAVLSVERRASVRPT"
     gene            complement(3548..4183)
                     /locus_tag="PLZ72_04470"
     CDS             complement(3548..4183)
                     /locus_tag="PLZ72_04470"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA35588.1"
                     /translation="MSDNPTRGIRFGSPRRSDLHEADGTGMGAPVPEWVEVSQTLDRW
                     FAFVDITGFTSYTEQHGTRAALDVLARFRSVVRQVTGRRGVRVLKWLGDGVMLVSTEP
                     GPLSAAVCEIAMRFEPDEFDVHAGIAGGSVLLFEGDDYVGPSANHAARLCDAAGPGEV
                     LALDVVGHLPDWVRIEDTVVVVADGIGEMRPAVRVSVSPEARPVAPSAGAA"
     gene            complement(4357..5022)
                     /locus_tag="PLZ72_04475"
     CDS             complement(4357..5022)
                     /locus_tag="PLZ72_04475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNA35589.1"
                     /translation="MARRSRGPTLVVERELWDAGHDVVVGVDEVGRGAWAGPLTIGAA
                     VLPRDRRVYGVRDSKQLTEIRREQLFERVGEWCVAWAVGHASHEECDRLGMSDAQRLA
                     ARRALDGLGVEPDRVLVDGRWDFVGGGRTRMLVKGDATCLSIAAASILAKVTRDRIMR
                     AGADSYPGFDFDRNKGYPCPRHKLALKGYGPTAIHRRTWAFMDGTPWGARRPGPDDAP
                     TLF"
     gene            5266..5484
                     /locus_tag="PLZ72_04480"
     CDS             5266..5484
                     /locus_tag="PLZ72_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35590.1"
                     /translation="MDDAVPPGPETTAVDLRLDAGTTVEVQCRFDQRWSGGFTVVDVT
                     DEGYRIRRQSDGSVLPAWFPREQLRPSC"
     gene            5642..8500
                     /locus_tag="PLZ72_04485"
     CDS             5642..8500
                     /locus_tag="PLZ72_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNA35591.1"
                     /translation="MTRVDIVPHTHWDREWYSPYPTFRLRLVDLIDELLPRLEADPDF
                     GHFQLDGQMAVVDDYLGLRPDAAERIRALAADGRLSMGPWYVLPDEFLVSGETHVRNL
                     QLGMRRAADFGGAMAVGYLPDMFGHIAQMPQILTQFGFDDAVVWRGVPSSVTGPAFWW
                     IAPDGSRVRATYLPAGYGNGSGLPADPDGVRDQIELFRTLQGDLVGDPVLVMAGMDHE
                     VPPAHLSAVVAELNAAEADRADGETYHLRIRSLADHLADTARDHLGEIGGEMRSGYRA
                     NVLMGVASNRVDVKQSAARAERLLERVAEPLAALWMGDEHARWQPVLDLAWLDVIRNA
                     AHDSICACSHDEVVDAVRHRYAESIRLARGVADRAVAAGALGPSTPGPVVLNATARTR
                     TGMVEIDVAGVEQTGDGTSTVQVLIDRPEVEEIEGAVGADAPMRLAMASMAEHPDTRK
                     VRLDRSVPGVLTAHLLARRSRAERDRDPDPEPAGTAEELLSPADALATVGTLVAEQPD
                     LELRLVVHRSTPSRRVLTLAGDVPGFGWALWSPVVARHPVRPHGEFGLTNGLVDVEAD
                     PATGTFALDGLAGFGRLVDDGDAGDTYNWCPPEHDVVIDRPQSVVVTVAEAGPIRGRL
                     VIRSTYVLPERCAIDGRDDMVTGWSGGESYRRVGEVTQVITTTVEVRADDPAVRVTTA
                     WDQRARDHRLRVHLPLPTPATHSEAEDAYAVVRRPLWAEGGPNEWGVPTFPSRRFVRA
                     GGLTVTHEGLCEYELVDLLTPDGRTLPDTTGLAAADAASLGTAPPEGTTASAVALTLV
                     RSTGWLSRGPMPSRPQPAGPFDRLEGSQTLRPLELRYAIQLDTTAGALEPYALADHVW
                     NPLLSAVAVGGGDLGDRGTHLEVQAVPAGAVEIDAVLRDHHGRLLVRAHAVGDDACTL
                     TLPGRSGAVIDLLGREVDTFAGSIEVDPHRIVTLRLER"
     gene            8899..12357
                     /locus_tag="PLZ72_04490"
     CDS             8899..12357
                     /locus_tag="PLZ72_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNA35592.1"
                     /translation="MDATAAEPTPRPSGAEPRLLLLGSVELRVDGERTALGGPVQRAV
                     LVALALQRDHPVEVDHLLADVWGPDTPERTARSLATLVSRLRRMLEPLGSSIEHEHGA
                     YVLRRAPPTDVDLFGEHVERARRLVAAGDPAHALTELDRALVLWRGEPLGGLTAPFVD
                     ERRSQLVEQRWVVENERAALLTSVGAHDRAVATLEEMTAETPYSERRWAMLIGALHAA
                     GRRRDALLAYRRIDLRLREDIGIDPGPELRAAEMRVLEEDPLDPTPTEAGGAAARGAG
                     ALPGRHRELLRLGSVLARSRDRGTSTVIVGEAGIGKSTILGRVVAAAGDQQRVVVGRC
                     TRGSTSVTSVLVAPIVSALRGLGGGDPLDHWEELGPEGEDLRRLATPGAGFDPRLLGM
                     LEQRIVACTSAALHRLLDEGAVMVAVDDVHWADRVTRRCIDELMSAFADRPLSVLLCS
                     RPAERTPTDDGWVADLLDRPGLEVIRLGPIPDADIGRILAQHGAPAERIEDLVAATGG
                     IPMLAVELASLARRGEAISTHIPDRLRHILDLRFDALPDSALRLVRLVALERPTGSPS
                     VMGAALGLDPLDTADLIDAATASGAVAIAADGSIGFPHELIRAYFLDRLGAQASKVAR
                     TALLEAHVARDEMGAAARHAEVLGDDIAAERRFAVLVPAARDALDEEATEDAHRWAHL
                     ALSLGPPDGPLSSDDIDRHLVLGTVLVSLGSFVQARPVLLRTAEAARDSGRWSVVADT
                     LEGLGRIGQPHQGPDLEAYETLVSDALGGLVDDDERLGRLESLAFHIHSGPDPVRAER
                     HLERLAELARREPALRHVLDVCRFRHEVEHGSDVDRCVRTGPRLADRMRSSGDEFAAL
                     IVDALTLTAQLRAGRPVAPVDVDAAADRAAALGRPDIGLMCELITSAVATWSRPTHAA
                     DAIVTAASDLAFSTGDSAHALTAVLHTFSLRREQGRASIMLPALLALSGTYDHPALDC
                     LIGLCHREAEDTDAARERLVATWDGLAELERSAWEHTPLVALAVELAVELGEPIPPAL
                     RDRVDQALLPTSGSMIVFAGVGMHLGPGDLHLGRLAGDGGDPAEAVESLRSAAALARR
                     AGLDLWACWCDVWLAIELCRATEPTAVDEATALLAGARRAARRNGWVRLGRAVEQLSG
                     DRSAFS"
     gene            12434..13732
                     /locus_tag="PLZ72_04495"
     CDS             12434..13732
                     /locus_tag="PLZ72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA35593.1"
                     /translation="MTTDALLPPVTDAAADDLLVSTVLTDEGRQDPYPAYAALRAGGT
                     DAPGRWRTAFGSLGLAGYDDCLEALRHPRLGRPEPDMDLPAGVGGQARGRDEEGASSM
                     LFLNPPDHTRIRGLVSRAFTPRRVEELRPRIEALLTPVLKRFAEHGGGDVMADLAIPF
                     PVAVISELLGVPREGNEHIQPLVRDVTSFIDAAADDAALERAAASGEELQRYFTELIE
                     DKRSHPDDRLLTALIQVEEAGDRLSHGELLTNTVLLYAAGFETTSNLIGNGLRLLLNN
                     PDQMARLQERPELLPSAMWEILRADSPVQLNSRVTLEEVELFGQRWPRGSSFIVLQGS
                     ANHDESVYADAAAFDVARFHAGSGSEPAPPPLSFGWGAHHCLGAHLARAEGEIAFGAL
                     LRAFRSIELDADALPGGVPHYRPSFTLRGLDALPVVVTPA"
     gene            complement(13762..14559)
                     /locus_tag="PLZ72_04500"
     CDS             complement(13762..14559)
                     /locus_tag="PLZ72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNA35594.1"
                     /translation="MTRGDRRRNGRPRGRVGVAVLTSLLAVSLSALGGCTPEQFQRWW
                     VDGGRAPLPEPQLSQMAHAATLHWDEVFRKGRFRADVAPIDAALAARMTPTSWRPGCP
                     VGLGDLRHIRASYMGFDGLEHVGEIVVHADAVAVTVALLRNMWDARFPLRSLRLIDDF
                     GGSDLASIAADNTSAFNCRAVTGGTGWSVHAFGRAIDINPVENPYVTRSQVLPDAGRA
                     FLDRTDRRPGMIVAGDVVWNTARFVGWGWGGDWSALKDYQHVSASGG"
     gene            complement(14556..16511)
                     /locus_tag="PLZ72_04505"
     CDS             complement(14556..16511)
                     /locus_tag="PLZ72_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35595.1"
                     /translation="MTAAADQPVLDPPDIRVRPRSVHRAARHGHTVPEREWSPLGTLD
                     SGVIGRVDGAGSVQLDGARWSLDWWIGAEDRWHHPSREASVRQRCVGGSPVVETAVRV
                     PGGDVVSRSYAVQAHAGDWRGTAVVVEVENRTAVPVALAFVARPWVLDGPGALGSVSS
                     EGSTVTLDDGRRVLLARDPARTVVDTGDRVAARLDAGDDDHGPAGATSEKDDLEAAFI
                     VPLTHTAVARVLLPVGAGSGDAVPTGPWSAPDLDAVVAGWSAHRRAGTGGGEPSVSLP
                     EPDWDDALEWAGSVLRVAGPDEVGSCLDRHRRVPNGPSAAVRSAEVTEALARLSAPDA
                     SVPVARGLAEALRLGGEVRLGDRTDGTVALLHAVGAVLAGQTDPDSEVAQEFVSPAAV
                     AIRRLRKGRGLAADDDVTGLRSSAVRALGRVAAALDRLGQPEVAADARSVANVLAAAA
                     SAVWTAASGTGPDASGAEPGVRSPLETALAARSALRRGDGAVALANLRSAWSGRVGSG
                     RSDAEIRPSEDGPEHPVGVLGFDVAELAARTNALIDLLVADGPEGPALLTAWDQTWAG
                     QPIEAHHLPSAWGPTSFAVRWHGDRPALLWEVDPGGGDEPVLTAVGLDPTWVGRGPEG
                     EGLLEAPKVESVVQIRTGGIRRRGSSG"
     gene            complement(16576..17586)
                     /locus_tag="PLZ72_04510"
     CDS             complement(16576..17586)
                     /locus_tag="PLZ72_04510"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNA35596.1"
                     /translation="MRIKVAVVGGGSWGTTVAHLASDHVPTVLWARRDDLADEVNSRH
                     TNERYLPGHQLNPALRATTDLAHAVAEADVLVMGVPSQGFRSALEQCAEHVRAWVPVL
                     SLTKGLEQGSRLRMTQIINEVLPGHPAAVLTGPNLAKEILVGDAAAAVIATPDLIVAN
                     HLQRIFHQNLFRVYVNPDVIGCEVAGALKNVIAIAAGMADGLGTGDNTKAAVITRGLA
                     ELSRLGEAMGGQFATFSGLAGMGDLIATCMSTQSRNRHVGEQLGRGRTIDEVIADMNM
                     VAEGVKTSRVVMELAAEYGVEMPICAEVHAVCHEGATAQDAYRGLIRGQDGGVEHHSL
                     AY"
     gene            17628..18254
                     /gene="cofC"
                     /locus_tag="PLZ72_04515"
     CDS             17628..18254
                     /gene="cofC"
                     /locus_tag="PLZ72_04515"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNA35597.1"
                     /translation="MHPDRDPPPVAAVLVPVKAFGRAKGRLAEVLDDDARARLARAMA
                     DRVLAAAHPLPAAVVCDDDDVAVWAREAGARVIRADRTGLNAAVGLGVEELTRRGVTR
                     VVIAHADLPFAVGLAALAVAEPTEVVLVPDRRGDGTNVMSIPTGAGMTFHYGPGSFDA
                     HRAAAARCDLAVRVLDSGPLGWDVDEPDDLDPPPEWGTVAEPATGTAR"
     gene            18251..19039
                     /locus_tag="PLZ72_04520"
     CDS             18251..19039
                     /locus_tag="PLZ72_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNA35598.1"
                     /translation="MSDLVDPLPPGGPTSRDLPTPDRALVIVAHPDDAEFQAGATLAK
                     WAAAGCRVTHLVLTDGSKGTWDPDQDPAALVAARHDEQRAAATVLGAEDPIFLDLIDG
                     ELVSGIVERGRVAEVIRRVRPDVVVGHDPWTRYRLHPDHHAAGRLCVEGVVAARDPGF
                     HREQLAAGLTPHRPDALLLFEPDVANHAETVGETDHARRLGALGAFSSQIATTHLYKV
                     ESGAEPGADPAEAWHARERERLATPGRWAGCELAEPFRLIVDQL"
     gene            complement(19083..19952)
                     /locus_tag="PLZ72_04525"
     CDS             complement(19083..19952)
                     /locus_tag="PLZ72_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35599.1"
                     /translation="MSSTPGRLRTQTVRVLMLGGLLLLSGTVIGLAAAYPLTFINYDD
                     SSAVRLDQPRLAGQVDVDAAVVSLDDLPKGWEPGDPALGGYGILNSQFCGETVDPPAP
                     LSEARTAVFKDPTNDSVLIAQALHVDKWQSASTYVRDVSDALGECSQFYRSGPGGKFK
                     VQIKEPSESAPVADDFVAATYVDAKGDGTQEWAIFAVGDVVVSVMHTGPTRPEPPFMN
                     DVIERVLARIDPTDFAPGGIAPTTTVAGADGSTPSTLDSGSADETEEAPAPGDPTGAP
                     AVPTTSIVTGGGD"
     gene            complement(19957..21711)
                     /gene="metG"
                     /locus_tag="PLZ72_04530"
     CDS             complement(19957..21711)
                     /gene="metG"
                     /locus_tag="PLZ72_04530"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNA35600.1"
                     /translation="MSRHLITSALPYINGVKHLGNLVGSMLPADVYARYLRAAENDVL
                     FICATDEHGTPAELAARAAGLDVAEFCAEQHEIQKDLGERFGLSFDHFGRTSRPPNHR
                     LTDHFARRLKAQGYTEVRSTEQVYSIADARFLPDRYVVGTCPNCGYERARGDQCENCG
                     KPLEPTDLIEPRSAVSGSSELEVRSSAHVFLLQSRLAERIRAWIDTKDDWPQLTRSIA
                     YKWLDEGLEDRGITRDLEWGIPVDPDDFPEVAGKVWYVWFDAPIGYLGATREWADLQV
                     GPEDAEALFERWWRNDRGAADVTYTEFMGKDNVPFHTLSFPATIIGSGEDWKLVDRLK
                     SFNWLTYYGGKFSTSEHRGVFMSDALELLPADYWRWYLMANAPESDDASFTWELFGDA
                     VNKDLVGTFGNFVNRTVTQVVRHFGEQVPVGGAPGATEAELAQRAEARLAEYVGHLDA
                     LEFRKAAAALRALWAEGNLYLEQTEPWKTIKVDRDRTACTLRTALGLVLAHSVASAPF
                     VPATAERLRAAFPDAVGVPLRLDAGLAAAASEVLRPGADLVPPSLLFRKLDPDELAGW
                     EQRFSGGETTPEPADAQG"
     gene            complement(21778..22215)
                     /locus_tag="PLZ72_04535"
     CDS             complement(21778..22215)
                     /locus_tag="PLZ72_04535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNA35601.1"
                     /translation="MAAVEATSGSCPFCAIVAGLAPAAVVHADDVAVAFLDRTPLFPG
                     HVLVVPRDHVVTLVDLPTEKVGPYFEQVRAAARAVTDGTGAAGTFVAMNNVVSQSVPH
                     LHAHVVPRNRRDGLKGFFWPRTRYRDQDHVDEFAERIRSAWPT"
     gene            22292..22882
                     /locus_tag="PLZ72_04540"
     CDS             22292..22882
                     /locus_tag="PLZ72_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNA35602.1"
                     /translation="MSPPLYGLDIETDTSVDGLDPSVSPIVAVAVATPDGDRVFQGAE
                     DLVLHRTDELMSELDPGIVVTWNGSGFDLPFLAERAQRLGVTLGLDLWDDGPERTEPG
                     VVRGRWYAHDHLDGYRLYRADVGRSLGFPCGLKPLSRLVGLAPVEVDRSCIHLLDDAA
                     LEAYVASDARLARELVLRRWPVAERATDRCHPRPPD"
     gene            22975..23835
                     /locus_tag="PLZ72_04545"
     CDS             22975..23835
                     /locus_tag="PLZ72_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35603.1"
                     /translation="MSFGPFTRDLRPKALTDVEGALPRARIDSVLEVLSRLEPATVIT
                     LAPDGHDQFLLAVRDLPPDPRCAGAGLFGLEPPAPCTLAAVSFVGSSGAEPGEVRVDA
                     LVTDTGHVRSQVHLADGVQPVQGRAGGVVVDALHRLLGLPCPGPTPPLTAMAEGLWFI
                     EILRRDAAGAGLSWADVARAQTERRSTADADAGTGTPPSPEVVAASICELADGADWDH
                     LRRAAAIGRMSAPELDPDEAAWMDATMFARWMVESFPSRQQVLDRLERHGPTAASRGV
                     REVLDRIGPG"
BASE COUNT         3324 a         8922 c         8496 g         3141 t
ORIGIN      
        1 cggccagggc cttggccgcg aacgcggccc ggtcgacccg gtctgcgggc ggatcgagcg
       61 acatcgagac ccgcaggtcc accggcgcgg cgctgaccag cggtgggagt cgctcccggc
      121 ccgacgccag ctggtgcagg tccgctcccc gcgccccgaa gcgggcgagc acgtcggggg
      181 cctccagcgc ggcgaaccgg ccgagggttc gcagacccag gcgacgcagc acgtcgacca
      241 gctcggccgg ctcacgaccc agcacctcca gacgggacac cggcaacggg gacaggaacc
      301 ctgcggtggc ccccggcgcg accacgatcg gggcggcacc ggatgcgcgg cgacgggcct
      361 cgtcggccac cagccccgcg gcgcgggggc cgtcggcgat ccccacgccg acggtgaccg
      421 gccaggcgac gtcgagctcc gcctccaccg cccgccgcac tgcggccagc accttgtcgg
      481 ccagcgcccg gtcaccgccg tggtagcgcg acggcccccg ggtgggcacc aggcaccgac
      541 cggcctcgac gacctccagg cggggggcca ggtcctccag cgcggcgagc accgcctcga
      601 aggcccggac gtcgcgcgac tcgtcgctgt cgtgcaactc caccgaggga caccgggcct
      661 gggcctcgcg gcggcggagg ccggccacga ccccctcggc ccgcgccgcc gggctggcgg
      721 cgatcacccg gtgggcggcc atcaccgcca ccggccggtc cgcgggaggg ccgatggcca
      781 ccaccggcca gtcggcgcag cacaccgcca gggtccgcac cggggccccc tcggaccccc
      841 ctcccctccc gttctgtagt gcgagatcca ggtgttcacc tggatctcgc actacagaac
      901 cgggaggcgg ggtcatcggt ccggggacgg cagccacagg tccacccgac gtgggcggtc
      961 gaagccccgt cggccccgca ccgagatcgt cacccgtcgg gcgagcaggc tcccccaacc
     1021 ctctccgggt cccgaccacg acgacgactc gcacaccagc tccacgtcgg gtcccggccc
     1081 ggccccgcca ccgtcgaccc tcgatcccct cccggggtga tccgccagct tcagcagcac
     1141 cgcaccgcgc tcgcgggccc gggccaccag gcgacgcatc tcggtcccgt cgggacggtg
     1201 ctcggggccg atcagcacca ggtcgaaggc gtcgaccagc gcggcgagca ccgccggagc
     1261 gcgaccctcg tcgctgcgga tgaccgccac ccgctcgagc tccacccccg cctccaccgc
     1321 cgccgcccac cccatggtcg acgccccgac gcacgccgcc caggacccgg cccgggtcgg
     1381 gcccgccatc accgcctggg ccagcgtggt ggaacccgca ccgcggaacg ccaccgtgga
     1441 tccccgctgc aggccatcgg gtagcaactc ctccaaggag gacaggaccg gcaggctgcg
     1501 ctcccgggcc aacgtgagcg ggctcaggtc accggccagc tcccgcagtc gggccagctt
     1561 ctccgagcgg tcaccgcgga ccgggtcgat ggagcctcgg gtgatcgacg ggacggcccg
     1621 gagggcgggg cggagctgct cccgggggac cgctcccccg tccgacgcga gctccccggc
     1681 gaccatccac ccagtatcga acgcctgttc gatgacacgc aagtggtccc gcacgggcgg
     1741 gttcagccgg gcccgcgttc ggtcgggccc gcattcagcc tggactctcg ccaatgacgg
     1801 tcggtgaatc cggacccaga ccctgcatga tcgaacaggt gtgccatggg ttggtgggca
     1861 tggacaccgg gaccaccagc accgtggaac ggccagcagc tgctggcgcg gatcagtcgg
     1921 ccgacgcgcc gaaggacgcc gcgtcgggga cctcgacccc cgggcggtag taggtgtaga
     1981 gatcgccgat gatctcggcg atgccgtcgg tgtcggagcc ccgacccagg tcgacggtga
     2041 tcacgttgga gtagatcgac accgtctcga tgccgccccg ttcgaacaga cgacgggcga
     2101 gcacgtcggg cggtcggtcc ccttcgatcg gctggtcggc ccggtagcgc tcatggccca
     2161 tgccggtgag gatccggttg atctcgaagc gaacgatccc gggacggttc gacggcttct
     2221 cgaggacggt gacggactga cccataacgg cccgagcgta gtcgccgcgc cacggatccc
     2281 ccgcatcgcc gccaccggcc ggggttggcc ccctccgggg ggcggcgacc accatgaagg
     2341 cggtgagcac ccctcctgga cccgacacca ccgacgccgc cgccacgacc gacggggacg
     2401 acacgaccga cggggacgac gacgggacgc accgggtcac cgtcttcgag gcagtcggcg
     2461 gccagtcctt cttcgaccgg ctggtcgacc gcttctacga gcgggtcgag accgacgacg
     2521 tgctgctgtc gatgtacccc gagcccgacg acctcgcacc cgcccgacgc cggctcgcgc
     2581 tgttcctcgg ccagtactgg ggcggaccgg cggtctacgc cgaggagcgc gggcaccccc
     2641 gcctccggat gcgccaccag ccattcgtgg tcggcgaccg cgaacgccgg cactggctgg
     2701 ccgccatgga ggactcgctg accgagacga tcgtcgaggc tccgctcgag cccgagctgc
     2761 gcgacgccgt cgagcagcgc atgagggagt acttcgagat gtccgccgcc gcgatggtca
     2821 actcggaccg ctgacccgcc ggtccggcga ccggttcccc aaaacgatgg acggaccgca
     2881 cgaagcggtc cgtccatcac gagcgatagc gagggtcgtc ccgacatcga gccccatcac
     2941 tgccgatgga ggacgaccct cggtcacgtc gcccatggga gaggaagggt ggatgggagg
     3001 acgacgtagc catggccgaa gccacgcacg catcatgcac caattcgtgg ggggatgtca
     3061 gtcactgacc gaagatatgt ggatattcag gttgggcgga ccgaggcccg gcgctccacg
     3121 ctcagcaccg ccacgaccgc gagggcgatc gataccgccg ccaggaccgt gtgcacgacc
     3181 acgaacgccg cctcgtgatc gccgccgaag gcgatgtcgc cggcccggac cacccagatg
     3241 acgacggtgt agccggcgaa agcccgcacg gcgttgcgca gcatccgagc ggacgccgcg
     3301 ctgcgcatga cgcgatccct gtacagcagc gctccgagca ccagggcacc cacgacgaag
     3361 atccccgaca ggaccagcgg accgatgcgg tccgatccgc tcagggcggg gtccgtcacc
     3421 gcgttgcgga tgcgtccgat ccacacgaac agggtccaca ccacgaacgc cccgaccggg
     3481 acgagcaccg accgggtcct ggccggcccg gcggtcccgt cggtcgaggt cgcggtgtcg
     3541 gctccactca cgcggcgccc gccgagggtg cgaccggacg ggcctcggga gagacgctga
     3601 cgcgcaccgc cgggcgcatc tcgccgatgc cgtcggcgac gacgaccacc gtgtcctcga
     3661 tgcggaccca gtccggcagg tggccgacca cgtcgagcgc cagcacctca cccgggccgg
     3721 cggcgtcgca gagccgggcc gcgtggttgg ccgagggccc gacgtagtcg tcgccctcga
     3781 acagcaggac gctgccgccg gcgatgccgg cgtgcacgtc gaactcatcg ggctcgaagc
     3841 gcatggcgat ctcgcacacc gcggcgctca gcggaccggg ttcggtgctg accagcatca
     3901 cgccgtcgcc caaccacttg aggacccgca ccccgcggcg accggtgacc tggcggacca
     3961 ccgaacggaa ccgggccagg acgtcgagcg cagcccgggt gccgtgctgc tcggtgtagg
     4021 aggtgaaccc ggtgatgtcg acgaaggcga accagcggtc gagggtctgg gagacctcca
     4081 cccattccgg gaccggtgcg cccatcccgg tgccgtcggc ctcgtggagg tcgctgcgcc
     4141 gagggcttcc gaagcggatt cctctggtgg ggttgtcact cacagtggtc gcctctgacc
     4201 ggggttcggg gccaccgccc gctccccgga ccgacgggtg ggagctgtgg gctaacggcc
     4261 tcttgctcct gcaagcatag gtcgtcggag cgtccgggcc acgccgccgg cgatcggctc
     4321 cacaacagca cgggctcgga tcagaacagg ctcggatcag aacagggtcg gggcatcgtc
     4381 gggtcccggt cggcgggccc cccacggcgt cccgtccatg aacgcccagg tccgacggtg
     4441 gatggcggtc ggaccgtagc ccttgagcgc cagcttgtgt cgcgggcacg gatagccctt
     4501 gttgcggtcg aagtcgaagc ccggatagct gtccgccccg gcccgcatga tccgatcccg
     4561 ggtgaccttg gccaggatcg acgccgccgc gatcgacagg cacgtggcat cgcccttgac
     4621 cagcatccga gtccggccgc cgccgacgaa gtcccaccgc ccgtcgacga gcacccggtc
     4681 cggctccacc ccgagcccgt cgagcgcccg gcgagccgcc aggcgctggg cgtcggacat
     4741 gccgagccgg tcgcactcct cgtggctggc gtgacccacc gcccaggcga cgcaccactc
     4801 gccgaccctc tcgaacagct gctcgcgacg gatctcggtc agctgcttgg agtcccgcac
     4861 gccgtacacc cgacggtccc tcggcagcac ggcggcaccg atcgtcaagg gtcccgccca
     4921 tgccccgcga ccgacctcgt ccaccccgac gacgacgtcg tgaccggcgt cccagagctc
     4981 tctctccacc accagcgtgg ggccacggga ccggcgggcc atgggcgaac ggtagcggcc
     5041 ccgaaccgtc gtcgaggacc gctcaagatc accacccccg gaccgacgga gaagcgggga
     5101 ggaggccgga ccggcatcgg ggggaccgac cgtcggaccg ggcgcctgaa gggaaagtcg
     5161 acggaggggc ttgagttcga ccccctcgtc ggccgaagaa cgggggtagc catgctgagg
     5221 accacccgac cggacccggt cgacctcggg gaggagggac caccgatgga cgatgccgtg
     5281 ccgcccggcc cggagacgac cgcggtcgac ctgcgcctgg acgccggcac gaccgtcgag
     5341 gtccagtgcc ggttcgacca gcgctggagc ggcggcttca ccgtcgtcga cgtgaccgac
     5401 gagggctacc gcatccgtcg ccagtccgac ggatcggtgc tgcccgcgtg gttcccccgc
     5461 gaacagctcc gacccagctg ctgacggcgc gaccaccgac acgctccgac accggcccgc
     5521 cgtcgagcag ggcgccgggc cgagctggca ggctgcgggc gtgaccgacg ctcgccccga
     5581 acccacccct caccccgtcc cggcaccggc aacggcaacg gcaacggcaa cggagagcac
     5641 ggtgacgcgg gtcgacatcg tgccccacac gcactgggac cgcgagtggt actcgcccta
     5701 cccgacgttc cggctgcgac tggtcgacct gatcgacgag ctgctccccc gcctcgaggc
     5761 cgaccccgac ttcggccact tccagctcga cggtcagatg gcggtcgtcg acgactacct
     5821 cggtctgcgc cccgacgccg ccgagcgcat ccgggcgttg gccgccgacg gtcggctgtc
     5881 gatgggcccg tggtacgtgc tgcccgacga gttcctggtc tcgggcgaga cccacgtccg
     5941 caacctccag ctcggcatgc gacgcgccgc cgacttcggc ggggcgatgg cggtgggcta
     6001 cctgcccgac atgttcggcc acatcgccca gatgccccag atcctcaccc agttcggctt
     6061 cgacgacgcc gtggtgtggc ggggggtgcc gtcctcggtg acgggcccgg cgttctggtg
     6121 gatcgccccg gacggatccc gggtccgggc gacgtacctg cccgccggct acggcaacgg
     6181 ctcggggctc cccgctgacc ccgacggcgt gcgcgaccag atcgagctgt tccggaccct
     6241 gcagggcgac ctggtcgggg accccgtcct ggtcatggcc ggcatggacc acgaggtccc
     6301 gcccgcccac ctcagcgcgg tggtcgcgga gctgaacgcg gccgaggccg accgagccga
     6361 cggcgagaca taccacctgc ggatccgctc cctggccgac cacctggccg acaccgcacg
     6421 ggaccacctc ggcgagatcg gtggcgagat gcgctccggg taccgcgcca acgtgctgat
     6481 gggggttgcg tccaaccggg tcgacgtcaa gcagtcggcg gcccgcgccg aacggctgct
     6541 cgaacgggtg gccgaaccgc tggccgccct ctggatgggc gacgagcacg cccgctggca
     6601 gcccgtgctc gatctggcgt ggctcgacgt gatccgcaac gcggcgcacg actcgatctg
     6661 cgcgtgcagc cacgacgagg tcgtcgacgc cgtccggcac cgctacgccg agtcgatccg
     6721 actggcgcgg ggggtggccg accgggcggt cgccgccggt gctctcggtc cgagcacccc
     6781 ggggccggtg gtgctcaacg cgaccgcccg cacccgcacc gggatggtcg agatcgacgt
     6841 cgccggtgtg gagcagaccg gcgacgggac ctccacggtc caggtgctca tcgaccggcc
     6901 cgaggtcgag gagatcgagg gggctgtcgg agcggacgcc ccgatgcgcc tggcgatggc
     6961 gagcatggcc gagcaccccg acacccgcaa ggtccgcctc gaccgctcgg tccccggcgt
     7021 cctcaccgcc cacctgctcg cccgccggtc acgcgccgag cgcgatcgtg accccgaccc
     7081 ggaacccgca gggaccgccg aggagctcct gtcccccgcc gacgcgttgg cgaccgtcgg
     7141 caccctcgtg gccgagcaac ccgacctgga gctgcgcctg gtcgtgcacc gatcgacgcc
     7201 gtcccgacgg gtcctgaccc tggccggtga cgtccccgga ttcggctggg ccctgtggtc
     7261 accggtcgtg gcccgacacc cggttcgtcc gcacggcgag ttcggcctga ccaacgggct
     7321 cgttgatgtc gaggccgatc ccgcgaccgg cacgttcgcg ctcgacgggc tcgccggctt
     7381 cggccgcctc gtcgacgacg gcgacgcggg tgacacctac aactggtgcc cacccgagca
     7441 cgacgtcgtc atcgaccgcc cgcagtcggt cgtggtcacc gtcgccgagg ccggccccat
     7501 ccgggggcgg ctcgtgatcc gctccaccta cgtgctgccc gagcgctgcg ccatcgacgg
     7561 tcgcgacgac atggtcaccg gctggtcggg cggcgagtcc taccgccggg tcggcgaggt
     7621 gacccaagtc atcaccacca ccgtcgaggt ccgcgccgat gacccggccg tgcgggtcac
     7681 gaccgcctgg gaccagcggg cccgggacca ccgactgcgg gtccacctgc cgctgccgac
     7741 gcccgcgacc cacagcgagg ccgaggacgc ctacgccgtg gtgcgccgtc cgctgtgggc
     7801 cgagggcgga ccgaacgagt ggggggtgcc caccttcccc tcgaggcgct tcgtgcgcgc
     7861 cggcgggttg accgtcaccc acgagggcct ctgcgagtac gagctcgtcg acctgctcac
     7921 ccccgacgga cggacgctcc ccgacaccac gggcctggcg gcggcggacg ccgcgtcgct
     7981 cgggaccgct ccacccgagg gcaccaccgc gtcggccgtc gcgctcaccc tggtgcgctc
     8041 gaccggttgg ctgtcccgcg gcccgatgcc gtcacgcccc cagcccgccg ggccgttcga
     8101 ccgcctcgag ggatcccaga ccctgcggcc gctcgagctc cgctacgcga tccagctcga
     8161 caccaccgcc ggggcgctcg agccgtacgc actggccgac cacgtgtgga acccgctgct
     8221 gagcgccgtc gcagtcggcg gcggcgacct gggcgaccgg ggcacccacc tggaggtgca
     8281 ggccgtgccg gccggagccg tcgagatcga cgccgtcctg cgcgaccacc acggccgcct
     8341 gctcgtgcgc gcccatgccg tgggtgacga cgcctgcacc ctcacgctgc ccgggcggtc
     8401 cggagcggtc atcgacctgc tcggccgcga ggtcgacacc ttcgccgggt cgatcgaggt
     8461 ggacccgcac cggatcgtca ccctccgtct ggagcgctga acccgccaac gatcggggtt
     8521 ccgacgaccg gacgcgaagg ggagcgacgc cgtggtgggt cccctcccac cggttccggt
     8581 cgggcccgat cgctgcggat cgggggatcc gcaccttcct ccctcaggtc cgaccggccg
     8641 gttcgagtcc accacggcgc cgcacgcact cgggaatcgg ggcgagccgc cgtcgtgcgc
     8701 ccccgcagtg tgaccgactg ctgtcaacat cgggtcaacg ccatcgtcca cggcgctcgg
     8761 accggcgcac cggagccgac ggtatgtccg atcggacaac gcgaaccctg gccggcgtga
     8821 gtgctggaca gcgctcgcgc cgaggacgac gatctggtcg ggagcgacga ccgtccggcg
     8881 gtcgtgggag gaaccgaggt ggatgcaact gcagcggagc cgacgccgcg accttcgggg
     8941 gcggaaccac ggctgctgct gctcggctcg gtcgagctcc gcgtcgacgg cgagcgcaca
     9001 gccctcgggg gcccggtgca gcgggcggtg ctggtggccc tcgcgctgca gcgggaccat
     9061 ccggtcgagg tggatcacct gctcgccgac gtctgggggc ccgacacccc cgagcgcacc
     9121 gcccgatcgc tcgcgacgct cgtgtcgcgc ctccgccgca tgctcgaacc gctcgggtcg
     9181 tcgatcgagc acgagcacgg cgcgtacgtc ctccgacgcg caccaccgac cgacgtcgac
     9241 ctcttcggcg agcacgtgga gcgcgctcgt cgcctggttg cggccggcga tcccgcccac
     9301 gccctcaccg agctggaccg ggcattggtg ctgtggcgcg gagaacctct cggcggcctg
     9361 accgcgccgt tcgtcgacga gcgacggtcc cagctcgtcg aacagcgctg ggtcgtggag
     9421 aacgagcggg cggcgctgct cacctccgtc ggcgcccacg accgggccgt ggccacgctg
     9481 gaggaaatga ccgccgagac cccctactcg gaacgtcgtt gggcgatgct gatcggggcg
     9541 ctgcacgccg ctggccgccg tcgcgacgcg ctgctcgcct accgccggat cgacctccgc
     9601 ctgcgcgagg acatcgggat cgaccccggg ccggagctgc gggccgccga gatgcgggtg
     9661 ctcgaggagg atccgctcga tccgaccccg accgaagccg gcggggccgc ggcccgcggg
     9721 gccggcgcgc tgccgggtcg ccaccgggag ctgttgcgcc tggggtcggt tctggcccgc
     9781 tcccgcgatc gcggcacgag caccgtcatc gtcggcgagg ccggcatcgg caagtcgacc
     9841 attctcggtc gggtcgtggc tgccgcgggc gatcagcagc gggtcgtcgt cggccggtgc
     9901 acccgcgggt ccacctcggt caccagcgtg cttgtcgctc cgatcgtctc ggcgctgcgc
     9961 ggactcggcg gtggcgaccc attggatcac tgggaggagc tcgggcccga aggagaggac
    10021 ctgaggcggc tcgcgactcc cggcgcgggg ttcgacccgc gtctgctcgg catgttggag
    10081 cagaggatcg tcgcctgcac ctcagcggcg cttcaccgac tgctcgacga gggagcggtc
    10141 atggtggccg tcgacgacgt ccactgggca gatcgggtga cgcgccgttg catcgacgag
    10201 ctgatgagcg cattcgcgga ccgaccgctg tcggtgctgc tgtgcagtcg accggccgag
    10261 cgcactccga ccgacgacgg ctgggtcgct gacctcctcg atcggcccgg gctggaggtc
    10321 atccggctcg gtccgatccc cgacgccgac atcggccgca tcctggccca gcacggcgca
    10381 ccggcggagc ggatcgagga cctcgtcgcc gccaccggtg ggatcccgat gctcgcggtc
    10441 gagctcgcga gcctcgcccg ccgcggtgag gcgatcagca cgcacatccc ggaccgcctg
    10501 cgccacatcc tcgaccttcg gttcgacgcg ctcccggact ccgccctccg cctggtccgg
    10561 ctggtggcgc tggagcgacc gacaggatcg ccatcggtga tgggagccgc tctcggtctc
    10621 gatccgttgg acaccgccga cctcatcgac gcggcgacgg cctcgggagc ggtcgcgatc
    10681 gcagccgacg gctcgatcgg cttcccccac gagctgatcc gtgcctactt cctcgaccgt
    10741 ctcggagcgc aggcctcgaa ggtcgctcgc accgcgttgc tcgaggccca cgtggcgcgg
    10801 gatgagatgg gggcagccgc gcgccacgcc gaggtgctcg gcgacgacat cgcggccgag
    10861 cggcggttcg cggtcctggt ccccgcggct cgcgacgcac tcgacgagga ggcgaccgag
    10921 gacgcccacc ggtgggcaca cctcgccctc tccctgggcc cgccggacgg accgctgtcc
    10981 agcgacgaca tcgaccggca cctggtgttg ggcaccgtgc tcgtgtcgct cgggtcgttc
    11041 gtccaggccc gccccgtcct gttgcgcacc gcggaggccg cgcgtgattc cggccggtgg
    11101 tcggtggtgg ccgacacact ggaggggctc gggcgcatcg ggcaacccca ccagggaccg
    11161 gacctggagg cctacgagac tctcgtgtcg gacgcgctcg gaggcctcgt cgacgacgac
    11221 gagcggctcg ggcggctcga gtcgctggcg ttccacatcc actcgggtcc cgatcccgtc
    11281 cgggcggagc gccacctcga gcggctcgcc gagctggccc ggcgggaacc ggcgttgcgt
    11341 cacgtcctcg acgtgtgccg gttccgacac gaggtggagc acgggtccga cgtcgaccgc
    11401 tgcgtccgca ccggtccacg gctggcggac cgcatgcgtt cgagcggcga cgagttcgcc
    11461 gcgttgatcg tcgacgcact gacgctgacc gcacagctcc gagccgggcg cccggtcgcc
    11521 ccggtcgatg tcgacgccgc cgcagatcgg gccgccgcgc tcgggcgccc cgacatcggc
    11581 ctgatgtgcg agctgatcac ctcggcggtg gcgacctgga gccgacccac ccatgcggcc
    11641 gacgcgatcg tgaccgcggc gtccgacctg gcgttctcga ccggcgactc ggcccatgcc
    11701 ctcaccgcgg tgctgcacac gttctcgctg cgtcgggagc agggcagagc gtcgatcatg
    11761 cttcccgcac tcctcgccct gtccgggacc tacgaccatc cggctctgga ctgcctgatc
    11821 ggcctgtgcc accgggaggc cgaggacacg gacgcggccc gggagcgcct ggtcgccacc
    11881 tgggacggcc tcgccgagtt ggagcgctcg gcgtgggagc acacgcccct ggtcgcgctg
    11941 gccgtcgagt tggccgtcga gctcggcgag ccgatcccgc ccgctctccg cgaccgcgtc
    12001 gatcaggccc tgctgcccac ctcggggtcg atgatcgtgt tcgcgggagt cggcatgcac
    12061 ctgggacccg gcgacctcca cttgggccga ctggccggcg acggcggcga ccccgccgag
    12121 gccgtcgaga gcttgcgctc cgccgccgcg ctcgcccgac gtgccgggct ggacctgtgg
    12181 gcctgctggt gcgacgtgtg gttggccatc gagctctgcc gcgccacgga gccgactgcg
    12241 gtcgacgagg cgaccgccct gctcgccgga gctcgacgtg cggcgcgacg caacggatgg
    12301 gtgcgcctgg ggcgtgccgt cgagcagctg agcggggacc gatccgcgtt ctcatgaccg
    12361 tccgcgccac cagtggcgcg gacggtcacg aaaatcgaac ggacggaccc cggcgacggc
    12421 agactggtcg ccgatgacga ccgacgcact cctccccccg gttaccgacg ctgccgccga
    12481 cgacctgctg gtgtcgacgg tgctcaccga cgagggtcgt caggacccgt atcccgcgta
    12541 cgccgcgctg cgggctggcg gcaccgatgc acccgggcgg tggcgcaccg cgttcggcag
    12601 cctcggactg gccggatacg acgactgcct ggaggcgctg cgccaccccc ggctcggacg
    12661 acccgaaccc gacatggacc tgccggccgg ggtcggcgga caggcccggg gccgtgacga
    12721 ggagggggcg tcgtcgatgc tgttcctcaa ccccccggac cacacccgga tccgggggct
    12781 cgtctcccgg gcgttcaccc cgcggcgcgt ggaggagctg cggccccgga tagaggcgct
    12841 gctcaccccg gtgctgaagc gtttcgcgga gcacggcggc ggcgatgtca tggccgatct
    12901 cgccatcccg ttcccggtcg cggtcatcag cgagctgctc ggcgttcccc gcgagggcaa
    12961 cgagcacatc caaccgctgg tccgcgacgt cacgtcgttc atcgacgccg ccgcggacga
    13021 cgcggcgttg gaacgggcag cggcgtcggg cgaggagctg cagcggtact tcaccgagct
    13081 gatcgaggac aagcgctccc accccgacga ccggctgctc accgcgctga tccaggtcga
    13141 ggaggccggt gaccgactca gccacggcga gctgctgacc aacaccgtgc tcctctacgc
    13201 cgccggcttc gagacgacgt cgaacctgat cggcaacggc ctgcggctgc tgttgaacaa
    13261 cccggaccag atggcgcgac tgcaggagcg gccggaactg ctcccgtcgg ccatgtggga
    13321 gatcctccgc gccgactcgc cggtgcagct caacagccgc gtgaccctcg aagaggtcga
    13381 gctgttcggt cagcggtggc cccgtggctc gtcgttcatc gtgttgcagg ggtcggccaa
    13441 ccacgacgag agcgtctatg ccgacgccgc ggcgttcgac gtcgcgcggt tccacgccgg
    13501 gtcggggtcc gagccggctc cgccgccgct cagcttcggc tggggagcac accactgcct
    13561 gggagcgcac ctggcccggg ccgaggggga gatcgcgttc ggcgcgcttc tgcgggcgtt
    13621 ccggtcgatc gagctcgacg ccgacgccct gcccggtggg gttccgcact accggccgag
    13681 cttcaccctc cgcggtctcg acgcgttgcc ggtcgtggtg acgccggcct gagttggcca
    13741 cggccgcgag cggtcgggtg ctcagccgcc gctggccgac acgtgttggt agtccttgag
    13801 cgccgaccag tcgcctcccc aaccccagcc gacgaagcgc gccgtgttcc acacgacgtc
    13861 gcccgcgacg atcatgcccg gacgacggtc ggtgcggtcg aggaacgccc gaccggcgtc
    13921 gggcagcacc tgggaacggg tgacgtacgg gttctcgacc gggttgatgt cgatggcccg
    13981 cccgaaggcg tgcaccgacc agcccgtccc gccggtcacg gcccggcagt tgaacgccga
    14041 ggtgttgtcc gccgcgatcg acgccaggtc gctgccgccg aagtcgtcga tcagacgcag
    14101 gctgcgcagc gggaaccggg cgtcccacat gttgcgcagc agggcgacgg tgaccgccac
    14161 ggcatccgcg tgcacgacga tctcgccgac gtgctcgagg ccgtcgaatc ccatgtagga
    14221 cgcacggatg tgacgcaggt ccccgagccc gaccgggcag cccggccgcc acgacgtcgg
    14281 ggtcatccga gccgccagag cggcgtcgat cggcgcgacg tcggcccgga accgaccctt
    14341 gcggaacacc tcgtcccagt ggagggtggc ggcgtgagcc atctgcgaca gctgcggctc
    14401 cggtagcggc gcccgaccgc cgtcgaccca ccagcgctgg aactgctcgg gcgtgcagcc
    14461 acccagggcc gacagcgaca cggccaacag cgacgtcagg accgcgaccc cgacccgccc
    14521 tcgggggcgg ccgtttcgac ggcggtcccc acgtgtcatc ccgaggaccc ccggcgacgg
    14581 atgccgccgg tgcggatctg caccacggat tcgaccttgg gagcctcgag cagaccttcg
    14641 ccctcggggc cccggccgac ccaggtcgga tccagcccga ccgcggtcag caccggctcg
    14701 tcaccgcctc ccgggtcgac ctcccacagc agggcgggac ggtcgccgtg ccagcgcacc
    14761 gcgaacgacg tgggtcccca cgcgctgggc aggtgatgcg cctcgatcgg ctgtccggcc
    14821 caggtctggt cccaagcggt gagcaacgcg ggtccctccg gtccgtcggc gaccaggagg
    14881 tcgatgaggg cgttcgtgcg cgccgccagt tcggcgacgt cgaaacccag gaccccgacg
    14941 ggatgctcgg gcccgtcctc gctcggtcgg atctcggcgt cggagcggcc gctccccacc
    15001 cgtccggacc aggccgagcg caggttcgcc agcgcgacgg cgccatctcc tcggcgcagc
    15061 gcgctccggg ccgccaacgc cgtctcgagc ggtgatcgga caccgggctc cgcgccggag
    15121 gcatcgggcc cagtgcccga cgcggcggtc caaacggctg aggccgcggc ggccagaacg
    15181 ttcgccacgg accgggcatc ggcggccacc tcgggttggc cgaggcggtc gagagccgcg
    15241 gcgacccgac cgagggcgcg gaccgcggac gagcgcagtc ccgtgacgtc gtcatccgcg
    15301 gccagtcccc gacccttgcg caaccggcgg atggctaccg ctgccggcga cacgaactcc
    15361 tgggcgacct cggagtcggg gtcggtctgc ccggccagga ccgctccgac ggcgtgcagg
    15421 agcgcgacgg tcccgtcggt gcggtcgccc aacctgacct cgccgcccag tcggagcgcc
    15481 tcggccagtc cccgggccac cggcaccgac gcgtcggggg ccgacaggcg ggccagcgcc
    15541 tcggtcacct cggcggagcg caccgcggcc gacggaccgt tcgggacccg ccggtgacgg
    15601 tcgaggcacg atcccacctc gtcgggtccc gcgacccgca ggaccgatcc cgcccactcc
    15661 agggcgtcgt cccagtccgg ctccggcagc gacacgctcg gctcgccccc gccggtgccc
    15721 gcccgacggt gcgccgacca gccggcgacc accgcgtcga gatcgggagc gctccacggc
    15781 ccggtcggca ccgcgtcgcc ggatccggcg ccgacgggca ggagcacgcg agcaaccgcg
    15841 gtgtgggtga gcggcacgat gaacgcggcc tcgaggtcgt ccttctccga cgtggctccg
    15901 gcgggaccgt gatcgtcgtc accggcgtcc agacgagccg cgacccggtc tccggtgtcg
    15961 acgacggtgc gcgccgggtc ccgggcgagg agcacacgac gaccgtcgtc gagggtgacc
    16021 gtcgagccct ccgagctgac cgaacccagg gcaccgggcc cgtcgagcac ccacggccga
    16081 gccacgaacg cgagggcgac gggcaccgcg gtgcggttct ccacctcgac gacgaccgcg
    16141 gtgccccgcc agtcgccggc gtgggcctgc accgcgtagg agcgggagac gacgtcgcct
    16201 cccgggaccc ggaccgcggt ctcgaccacc ggggagccgc cgacgcagcg ctggcgcacc
    16261 gacgcctccc gcgacgggtg atgccaccgg tcctcggcgc cgatccacca gtcgaggctc
    16321 cagcgggccc cgtcgagctg caccgatccg gcgccgtcga cccgcccgat gacccccgag
    16381 tcgagggtcc cgagcgggct ccactcacgc tcggggaccg tgtggccgtg acgggcggcc
    16441 cggtgcacgc tccgcggacg gacccggatg tcgggaggat cgagaacggg ctggtccgcg
    16501 gcggcagtca tcgtggccgc aggttaggtc cccggacacg acgccccgaa ggccacgacc
    16561 gtcggcggta gaggttcagt acgccaggct gtggtgctcg acgccgccgt cctggccgcg
    16621 gatgagaccc cgatacgcgt cctgggcggt ggcgccctcg tggcacacgg cgtgcacctc
    16681 ggcgcagatc ggcatctcga cgccgtactc ggcggccagc tccatcacga cccgcgacgt
    16741 cttgacccct tcggcgacca tgttcatgtc ggcgatgacc tcgtcgatgg tgcggccccg
    16801 accgagttgc tcgccgacgt ggcggttgcg gctctgggtc gacatgcagg tggcgatcag
    16861 gtcgcccatg ccggccaggc cggagaacgt ggcgaactgc ccgcccatcg cctcgcccag
    16921 tcggctgagc tcggccagac cgcgggtgat gaccgctgcc ttggtgttgt cgccggtgcc
    16981 caggccgtcg gccatccccg ccgcgatcgc gatcacgttc ttgagcgctc cggccacctc
    17041 gcagccgatc acgtcggggt tcacgtacac ccggaacagg ttctggtgga agatccgctg
    17101 caggtggttg gccacgatca ggtccggcgt ggcgatgacc gcggccgcgg cgtcgccgac
    17161 gaggatctcc ttggccaggt tcggtccggt gagcaccgcg gcggggtgcc ccgggagcac
    17221 ctcgttgatg atctgggtca tgcgcaggcg gctgccctgc tccaggccct tggtcagcga
    17281 caggaccggt acccacgccc ggacgtgctc ggcgcactgc tcgagcgcag agcggaaccc
    17341 ctgcgacggc acccccatga ccaggacgtc ggcctcggcc acggcgtggg ccaggtcggt
    17401 ggtggcccgc agcgccgggt tgagctggtg gccgggcagg taccgctcgt tggtgtgacg
    17461 cgagttgacc tcgtcggcca ggtcgtcgcg ccgggcccac agcacggtcg gcacgtggtc
    17521 cgacgccagg tgggcgaccg tcgtccccca cgatccaccc ccgaccacgg ccaccttgat
    17581 cctcatggcg acaccctagg cagcggaccc gtcgtacgct gcgaccgatg cacccggacc
    17641 gcgacccacc tcccgtcgcc gcggtcctgg tcccggtgaa ggcgttcggg cgggccaagg
    17701 gacggctcgc cgaggtgctc gacgacgacg cccgggcccg actcgcccgg gccatggccg
    17761 accgggtgct cgccgcggcg caccccctgc cggcggcggt ggtgtgcgac gacgacgacg
    17821 tcgccgtctg ggcccgagag gcaggcgccc gggtgatccg ggccgatcgg accggcctca
    17881 acgctgcggt cggtctcggg gtcgaggagc tgacgcgacg cggcgtgacc cgggtggtga
    17941 tcgcgcatgc cgaccttccc ttcgccgtcg ggctcgccgc cctggccgtg gccgagccga
    18001 ccgaggtggt gctggtcccg gaccggcgag gcgacggcac caacgtgatg tcgatcccca
    18061 ccggcgccgg gatgacgttc cactacggac cgggttcctt cgacgcccac cgtgccgccg
    18121 ccgcccgctg cgacctggcg gtcagggtcc tggactcagg tccactgggc tgggacgtcg
    18181 acgaacccga cgacctggac ccgccaccgg agtggggcac cgtggcggag cccgcgaccg
    18241 ggacggcacg gtgagcgacc tcgtcgaccc gctgccaccg ggcggaccca ccagccgcga
    18301 cctgcccacc cccgaccgcg ccctggtgat cgtcgcgcac cccgacgacg ccgagttcca
    18361 ggccggcgcc acgctggcca agtgggccgc ggcggggtgt cgggtcacgc acctggtgct
    18421 gaccgacgga tccaagggca cctgggatcc cgaccaggac ccggccgcgc tggtggcggc
    18481 ccgtcacgac gagcagcgcg ctgccgcgac cgtgctcggg gcggaggatc cgatcttcct
    18541 ggacctgatc gacggggagc tcgtctccgg gatcgtcgag cggggccggg tggccgaggt
    18601 gatccgtcgg gtccgtcccg acgtcgtggt cggccacgat ccctggaccc gctaccgact
    18661 ccaccccgat caccacgccg ccggtcggct ctgcgtcgag ggagtggtcg ccgcccgcga
    18721 cccgggcttc caccgcgagc agctcgccgc cgggctcacg cctcaccggc cggatgccct
    18781 gctgctgttc gagcccgacg tggccaacca cgccgagacc gtcggcgaga ccgaccacgc
    18841 ccggcgcctc ggcgcgctgg gcgcgttctc gagccagatc gcgacgacgc acctctacaa
    18901 ggtggagtcc ggagcggagc cgggggcgga ccccgccgag gcgtggcacg cccgcgagcg
    18961 ggaacgcctg gcgactccgg ggcgctgggc gggctgcgag ctggccgagc cgttccggct
    19021 catcgtggac cagctctgaa ccggtcccgg ccgctgtcag tcgccgctgt cagtcgccgc
    19081 ggtcagtcgc cgccgccggt gacgatcgac gtcgtcggca ccgcaggtgc accggtcgga
    19141 tcaccggggg cgggtgcctc ctcggtctcg tcggccgagc cgctgtcgag cgtggagggc
    19201 gtcgagccgt ccgctccggc cacggtcgtg gtcggggcga tgccgccggg agcgaagtcg
    19261 gtcgggtcga tccgggccag caccctctcg atcacgtcgt tcatgaacgg cggctcgggc
    19321 cgggtcggac ctgtgtgcat gaccgacacc acgacgtcgc cgaccgcgaa gatcgcccac
    19381 tcctgggtcc cgtcgccttt ggcgtccacg taggtcgcgg cgacgaagtc gtcggccaca
    19441 ggagcggact ccgacggttc cttgatctgg accttgaact tgccgccggg gccggaccgg
    19501 tagaactgcg agcactcacc gagggcgtct gacacgtcgc gcacgtaggt gctggccgac
    19561 tgccacttgt cgacgtggag ggcctgggcg atgaggaccg agtcgttggt cgggtccttg
    19621 aacaccgcgg tgcgggcttc cgacagcggc gccggaggat cgacggtctc tccgcagaac
    19681 tggctgttga ggatcccgta gcccccgagg gccggatcgc cgggctccca gcccttgggc
    19741 aggtcgtcga ggctgaccac cgcggcgtcg acgtcgacct gaccggcgag gcggggctgg
    19801 tccagtcgca ccgcggagga gtcgtcgtag ttgatgaacg tcagcgggta cgccgcggcc
    19861 aggccgatca cggtcccgct cagcaacagg aggccgccga gcatcaggac gcgcacggtc
    19921 tgggtccgca gtcggcccgg cgtggagctc acaacgtcat ccttgcgcgt cggcgggctc
    19981 cggtgtggtc tctcccccgc tgaagcgctg ctcccacccg gcgagctcgt cgggatcgag
    20041 cttgcggaac agcagcgacg gcggcaccag atcggcgccg ggcctcagca cctcggaggc
    20101 agccgcggcc agaccggcgt cgagacgcag cggaaccccg accgcgtcgg ggaacgcggc
    20161 ccgaagtcgc tccgcggtgg ccggcacgaa cggggcggac gccaccgagt gggcgagcac
    20221 caggccgagc gccgtgcgca gggtgcatgc ggtgcggtcc cggtcgacct tgatcgtctt
    20281 ccagggctcg gtctgctcca ggtagaggtt gccctcggcc cacagggcac ggagagcggc
    20341 ggcggccttg cggaactcga gcgcgtcgag gtggccgacg tactcggcga ggcgggcctc
    20401 ggcgcgttgc gccagctccg cctctgtcgc gccgggggca cctccgacgg ggacctgctc
    20461 gccgaagtgg cgaacgacct gggtcacggt gcggttgacg aagttgccga aggtgccgac
    20521 caggtccttg ttgaccgcgt cgccgaacag ctcccaggtg aagctggcgt cgtcggactc
    20581 cggcgcgttg gccatcaggt accagcgcca gtagtccgca ggcagcagct ccaacgcgtc
    20641 ggacatgaac acgcctcggt gctccgaggt ggagaacttg ccgccgtagt aggtgagcca
    20701 gttgaaggac ttgagtcggt cgaccagctt ccagtcctcg ccggagccga tgatggtcgc
    20761 cgggaagctc agggtgtgga acggcacgtt gtccttgccc atgaactcgg tgtaggtgac
    20821 gtccgcggcg ccgcggtcgt tgcgccacca ccgctcgaac agcgcctcgg cgtcctcggg
    20881 gcccacctgg aggtccgccc actcccgcgt ggcgcccagg tagccgatcg gggcgtcgaa
    20941 ccacacgtac cagaccttgc cggcgacctc ggggaagtcg tcggggtcga ccgggatccc
    21001 ccactcgagg tcgcgggtga tgccccggtc ctcgagtccc tcgtcgagcc acttgtaggc
    21061 gatcgagcgg gtgagctgcg gccagtcgtc cttggtgtcg atccaggccc ggatgcgttc
    21121 ggccagccgc gactgcagca ggaacacgtg ggccgacgag cggacctcga gctccgacga
    21181 gcccgacacc gcgctgcgcg gctcgatcag gtcggtgggc tccaagggct tgccgcagtt
    21241 ctcgcactgg tcgccacggg cccgttcgta gccgcagttg gggcaggtgc cgaccacgta
    21301 gcggtcgggc aggaagcggg cgtccgcgat cgagtacacc tgctcggtgc tgcgaacctc
    21361 ggtgtagccc tgcgccttga gccgacgggc gaagtggtcg gtgagccggt ggttgggcgg
    21421 gcgtgaggtc cgcccgaagt ggtcgaagct gagcccgaag cgctcgccga ggtccttctg
    21481 gatctcgtgc tgctcggcgc agaactcggc gacgtccagc ccggcggcgc gggccgcgag
    21541 ctcggccggg gtgccgtgct cgtcggtcgc gcagatgaac aggacgtcgt tctcagcagc
    21601 gcggaggtac cgggcgtaca cgtccgcggg cagcatcgat ccgaccaggt tgccgaggtg
    21661 cttcaccccg ttgatgtagg gcagggcgct ggtgatgagg tgacgtgaca cgcgtgcgat
    21721 cggctccgtt cggcgacacg ccagactacc ggcggaaccg acggtgcccc ggacgcctca
    21781 cgtcggccag gccgagcgga tccgctcggc gaactcgtcg acgtggtcct ggtcgcggta
    21841 gcgggtccgg ggccagaaga accccttgag cccgtcgcgg cggttgcggg gcaccacgtg
    21901 ggcgtgcagg tgcggcaccg actgcgacac gacgttgttc atggccacga acgtccccgc
    21961 cgcgccggtc ccgtcggtca ccgcacgggc cgcggcgcgc acctgttcga agtagggacc
    22021 gaccttctcg gtcggcaggt cgaccagggt caccacgtgg tcacggggga cgacgagcac
    22081 atgccccggg aacagcggtg tccggtcgag gaacgccacc gcgacgtcgt cggcgtggac
    22141 caccgccgcc ggcgcgagcc ctgcgacgat cgcacagaac ggacagctgc ccgacgtcgc
    22201 ctcgaccgcg gccatcagtc gaccgtaccg ggaccggact gtcagcgcgt ggctgtcggg
    22261 actgtcagcg cggctgcgta cgatcgccgc tgtgagccct ccgctctacg gcctggacat
    22321 agagaccgac acctccgtcg acggcctcga cccgtcggtg tcgcccatcg tggccgtcgc
    22381 ggtggcgacc ccggacggcg accgggtgtt ccagggcgcc gaggacctgg tcctgcaccg
    22441 aaccgacgag ctgatgtcgg agctggatcc ggggatcgtc gtcacctgga acgggtccgg
    22501 cttcgacctg ccgttcctgg ccgaacgggc gcaacgcctc ggcgtcaccc tgggcctcga
    22561 cctgtgggac gacggcccgg agcggaccga accaggcgtg gtccgcggac ggtggtacgc
    22621 gcacgaccac ctcgacggct accgcctcta ccgcgccgac gtcggccggt cgctgggctt
    22681 cccctgcggc ctcaagccgc tgtcgcgcct ggtcggcctg gcaccggtcg aggtggaccg
    22741 cagctgcatc cacctgctcg acgacgccgc gctcgaggcg tacgtcgcct ccgacgcccg
    22801 tctggcccgg gaactggtgc tgcgacgctg gccggtcgcg gagcgggcga ccgaccgctg
    22861 tcacccccga ccgccagact gatgctgtcg cccggtgcac accgggccgg tcggacccgc
    22921 cgcaccgggt tcctcctgca cccacgacgg gtgcccgaca cctggaggaa ccccgtgtcg
    22981 ttcggacctt tcacccgcga cctgcgcccc aaggccctca ccgacgtcga gggagcgctc
    23041 ccccgtgcac ggatcgactc cgtgctcgag gtcctcagcc ggctcgagcc cgccaccgtc
    23101 atcacgctgg cgccggacgg ccacgaccag ttcctgctgg ccgtccgcga cctgccgccc
    23161 gatccccgct gcgccggagc cgggttgttc ggcctcgaac cccccgcccc gtgcaccctc
    23221 gcggcggtct ccttcgtcgg ctcctccggg gcagaaccgg gcgaggtgcg tgtcgacgcc
    23281 ctggtgaccg acacgggtca cgtccgttcg caggtccacc tcgccgacgg ggtgcagccg
    23341 gtccagggcc gcgccggagg ggtggtcgtc gacgccctgc accgactcct gggcctgccc
    23401 tgtccggggc cgacgccgcc gctgaccgcc atggccgaag gcctgtggtt catcgagatc
    23461 ctccggcgcg acgcagccgg cgcggggctc agttgggccg atgtggcccg ggcgcagacc
    23521 gaacgccgct cgaccgccga cgccgacgca gggaccggga ctccgccgtc gccggaggtg
    23581 gtcgcggcct cgatctgtga gctggccgac ggagccgact gggaccacct gcggcgggcc
    23641 gcggcgatcg ggcggatgtc ggcaccggag ctggatcccg acgaggcggc gtggatggac
    23701 gccaccatgt tcgcccgctg gatggtcgag tcgttcccca gccggcagca ggtgctcgac
    23761 cgactcgagc gtcacggacc caccgcagcg agccgcggcg tccgggaggt gctcgaccgc
    23821 atcggtcccg ggtgacccat gcccgacacc ccggtcagcc gcggcgaggc gtgcgccgcc
    23881 tag
//
LOCUS       DAOQOD010000027        23482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_739_length_23482_cov_11.418534, whole genome shotgun sequence.
ACCESSION   DAOQOD010000027 DAOQOD010000000
VERSION     DAOQOD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_23482_cov_11.418534"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLZ72_04550"
     CDS             <1..1116
                     /locus_tag="PLZ72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNA35604.1"
                     /translation="DAADLLAGAQRPFLFAGKGVLWAEASAAFVALGDHLGAAMSTTL
                     GARGAVPEDHPHYFTIADGNTLRAVRGDADVVLVVGARLGEYDGWGVPPAWGDSEDQR
                     TIQIDSDPMSIGLNRPVDLAVVADARGALEALLAAVAERVEARPGLEGAERYAAIHEQ
                     SVAEATPHVLAEPTVGVNPGQMVMALRELFPRDAVTVADGGNTTLWAVALNPVYVPDS
                     FLYSVKMGYLGTGLPFAIGAKLAAPERDVYLISGDGAFGFNAMELETARRVGAPVICV
                     VAVDGGWGMERTAHRFMGVPDDEAHGTDLSPEVRYDELARALGCHGEHVTAMDELAPA
                     IERARSSGLPAVLHVEVDPDINANPIGYEQFRSARTY"
     gene            1118..2137
                     /locus_tag="PLZ72_04555"
     CDS             1118..2137
                     /locus_tag="PLZ72_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35605.1"
                     /translation="MDDPPASVFVTGANGFMGRALAERYRSAGVDVSGVDVTADPDRG
                     VIAGDVLDPSGWADAIAPGAVVIHTAAIVSNNIATERAWEVNVVGTQRVLDAATGAGA
                     ERFVQISTMGVTRFAHTHAAAARRILPGRELDERWPLMPTGNPYTDTKITAEHLVLAA
                     HAAGRQACTIIRPADVYGPGCRPWVLEPLEAIRSGRMLLPARGRGLFTPIHIDDLLDG
                     VVAAAARPAAAGQIFHLGGEEPVTTLEYFGHLARMIGRPAPRTAPTAVAVAAAEATRW
                     VAVARRRHTEVGRGVMAMLAKTRAVSNEKAHRDLDWWPRVSLSDGMADVERWLRSEGL
                     LDDAR"
     gene            2217..>3556
                     /locus_tag="PLZ72_04560"
     CDS             2217..>3556
                     /locus_tag="PLZ72_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNA35606.1"
                     /translation="MSAPGQTGSDGTAVGAGTYRAEIRWTTHGVAHIRGESLPDVAFG
                     QAYAIAGHHLPTIADQLLKTRSERARHFGRGDNDCHVNSDFGYLAMDMTAWAQRMLAT
                     QPPSVVEVVEAYAAGLNRWLAEHGTADLPEWCRSAEWIRPVDAVDLFRLYADMMLMAS
                     GRNAAEFVGAAQPPGSPPVAHPERPLVPDDGPGSNGWALGGDVTADGRGAVVANPHFP
                     WYGDGRFWECHLTVPGELDVYGVCLVGAPGIQIGFNRDVAWTHTFSRGQRFNFYSLQL
                     DPSDPTRYRYGDEMRSMTPRVEAIEVLSSDGSLEKVERTLWSSHYGPMLDVPLLGWSD
                     ALAFSLADVNHGNDRILAQYLSMDVATDVASLRDSVRTHQGLPWVNVIAADRHGDVWY
                     ADPSPAPRLSAEAEAAFDRAVETDPITMLFYSQRVAILDGSDPANEWVDDPRSAL"
     assembly_gap    3557..3656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3657..4448
                     /locus_tag="PLZ72_04565"
     CDS             <3657..4448
                     /locus_tag="PLZ72_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNA35607.1"
                     /translation="PEDLERALLDNRSLLAEQLLDDVIDRCRRSGDATLADAAEVLSG
                     WDRAFDVGSRGAVLWREFLATFSEDDLRGGGSLFAVPFDVADPVHTPRDLAPAPESGP
                     DPVHTAMWSALDVLASAGIAADVALGDVQFIDRSGERIPMHGANEVEGIINVVAPVGA
                     FGGSDLEPRPSSGSPIPGRTERTGLCSTGYPITYGASTLMVIGWDDDGPVARGLLTYG
                     QSGDARSEHHVDQMREFSAKRLRPLCFHDADIDADPQLRVEVVEG"
     gene            complement(4516..4953)
                     /locus_tag="PLZ72_04570"
     CDS             complement(4516..4953)
                     /locus_tag="PLZ72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNA35608.1"
                     /translation="MHTIDVRPLPPPVRHQTIFETLDALGTGDTLRLVNDHDPAPLRH
                     QLEATRPHQFRWEYVQSGPEEWAIDITSVARVVDARPTLAAGGEPFGEIMAAAAEVGD
                     GEALVVYAPFEPVPLEGVLGEQGFAYVADQLDGGDWRTTFHRG"
     gene            5105..6472
                     /locus_tag="PLZ72_04575"
     CDS             5105..6472
                     /locus_tag="PLZ72_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35609.1"
                     /translation="MTVQPPTAPQIPATAPASAPTAAPFVPGATAAVPPASVPLGFLA
                     AAGVGLVAFGLAAWFSADRIVDMPTHPGSVAAVHVGMLAFLTTAVLGAAHQFSPVVGR
                     RPLRSVPAARVTLVGMVATAWMLPSGFAHGPEALVLAGGITGAATVLLAAWNLSGPLS
                     TRDGGVPLVGLRVSIAYLVLTVAFGVTYAIDRQTLWFPLLPNRVLAHAHLGLIGWLGI
                     TYVSVAEKLWPMFLLAHRPRARAGAWAVGLLGSGVGGLAVGLLLESPIVVALGGTAVV
                     VGFGCHIASLVGVVRARRRPLELLHRFLFTSTAFLVVAIALGVAAALAPVDYGTRSRL
                     VSAEVASLAAWLGLAVIGHVHKIVPFIGYSALRARGVTHAPSGKPLMFADLFHPGIAR
                     ATLVASGAGFALAVIGILVGSSTVIALGGVGIATAGVLVTANLVSGPRRVTRASARSA
                     PATAA"
     gene            6555..7898
                     /gene="gdhA"
                     /locus_tag="PLZ72_04580"
     CDS             6555..7898
                     /gene="gdhA"
                     /locus_tag="PLZ72_04580"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNA35610.1"
                     /translation="MTIDAKLQQVWTDVVSRNPAEPEFHQAVHEVLTSIGPVLAKHPE
                     LARLKSIERICEPERQIIFRVPWMDDDGEIQINRGFRVESNSALGPYKGGLRFHPSVN
                     LGIIKFLGFEQTFKNALTQMPIGGGKGGADFDPKGRSDGEIMRFCQSFMTELYRHLGE
                     YTDVPAGDIGVGAREIGFLFGQYKRITNRYESGVLTGKGITWGGALVRTEATGYGATF
                     FAEEMLKVRGTSLDGRTCVVSGSGNVALYAIEKLGQLGATVVACSDSEGYVVDKSGLD
                     LELLKEIKLNARGRVSEYTQQHPDAQFVTDGSIWDVPCDVALPCATQNELTGADAATL
                     IRNGVIAVVEGANMPATPDAVSAFLEAGVAFGPGKAANAGGVATSALEMQQNASRDSW
                     TFEHTEERLARIMAGIHDACYETAEEYGAPGNYVVGANIAGFLRVSQAMLALGLI"
     gene            complement(7951..9897)
                     /locus_tag="PLZ72_04585"
     CDS             complement(7951..9897)
                     /locus_tag="PLZ72_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNA35611.1"
                     /translation="MGASTMAAVLSSGGVSRGGVSRGRVVGGLVALVMVATAVACSPP
                     APLDLTGTDGPYGVTVTRNLTIPLDDGRVLRADLYEPTDPGTGTAAAGPFPVIVGMTP
                     YGKSGAAGASIPGGNGVNLQLVRHGYLALAVDVPGTGISDGSFDLFDPAEATAGAQIV
                     RWAAALPRSTGRVGMLGLSYMAIVQLFTAAAVGPDSPLKAIFPMAATVDPYRDLFTSG
                     GALNVLSPLGLLFGYGITRSVTPFAEAWDDPALAMQHAAANLEQMGRFEGVIAQDMFD
                     NGPRRYDGDWWQLRRPERVLQRIADNGVAVHLVGGLYDVFQRGEPLLYSGLQNAAAGR
                     PVEAPMLAGQPVSDRFRLLTGPWTHGTIGEGIDLSAVQLAWFDRWLKDVPHDERQADG
                     EQSADGDPAAPLRVIEPGGTTYDAATYPLADAPLERLYLRADGRLDPEPPGTDEPSDG
                     VDYFPLGDPCTASTVQFAAGLGAKDCLVPRPTAPEVPNEVTYSTDPLAEPLTLAGPVL
                     ASLAATSNSPDVLFSVTVEDVAPDGTSMGLTGGAILGSMRALDDSRSWTDAQGRLVRP
                     HQLLTAAARQPVPVGEAVRYPVEVRPAFVTVPAGHRLRLRVSTADFPHFLPLADMAGL
                     AGGHQDLHHSVAQPSYLDLSVRRP"
     gene            9970..10863
                     /locus_tag="PLZ72_04590"
     CDS             9970..10863
                     /locus_tag="PLZ72_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35612.1"
                     /translation="MDEQRARTHPSLAGRRVLITGAARGIGAALAQRLHDRGARVALA
                     GLEPDQLRATADGLGAPFWDCDVTDRSRVARVVDEAAAELGGLDVVVANAGIAAQLPL
                     VDGDPTIFEKTLAVNTLGTYYTLREAGRHVAHPGGYAVAVSSLAAAVHVPLMGAYSAS
                     KAAVEALGNTLRQELRPSGARVGVAYFAELDTDMTSRGFGTEAAAAFLDGRSLTRVTP
                     LVKGIDALERGITRRSRIICAPWYVAPILPTRRVAQAVVELATRRRLAPVLDIARLER
                     VELTTPQPERSPEMPAQEDRP"
     gene            10860..11456
                     /locus_tag="PLZ72_04595"
     CDS             10860..11456
                     /locus_tag="PLZ72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA35613.1"
                     /translation="MSDAVTPTDSRTAGPTTGPRVSPGDRRQVGRLNHAIASALGRAA
                     GTAAPNLFLTLGRHRRLFRGWLHFAGTLMPGGRLSRRESETVILRVANRRQCDYEREH
                     HRRLGLRAGLTVDDVDALGAPDTATDPRWSARQAAMIAAVDQLLEHRDLDDQAWASLR
                     RRLDDRELIELVMLVGHYDMLATAITTLRIQPDGPRTR"
     gene            11453..12094
                     /locus_tag="PLZ72_04600"
     CDS             11453..12094
                     /locus_tag="PLZ72_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35614.1"
                     /translation="MTDAVAASTDATRRLPRGRHDLTREQIEEDQRLRILLGFAGAMA
                     SNGYARTPIAAVIAHAGVSRETYYRLFSDKLDGFLAAFDLVSTVLLAELDRAVEGPGT
                     PLDRIDDGVRRYLEVLAEHRGEARLFLVEAFAAGPVAIERRAEVQERIAARIAEVLEV
                     GSESGRVTCRMLVAAVSALIVTPLVADDHDAILALGPLISAELRRYHAAGLLS"
     gene            complement(12207..13919)
                     /locus_tag="PLZ72_04605"
     CDS             complement(12207..13919)
                     /locus_tag="PLZ72_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNA35615.1"
                     /translation="MAQLEVRLLGRPAVLVDGAEVPPPKGAKPWALLAYLVAEDRPRP
                     RAELAELLFSGASDPLGALRWNLAALRRLLDRPDVVKGDPIGLDLSDARIDVRDVLSA
                     HGPPTDPAPDGLLLAGLSFPDSPRFELWLAGERARLSRLQMSRRREAVLRGLASGDHE
                     TALDLATALVATDPLDESSHALLIRAHAVAGDVGMARSQYERCREMLRRELSVEPGGA
                     VVAAVHFASEVAERRQPVDVATVEARLAIAWQSFLAGSVDHAIDLGRDASSMADREHD
                     LGLRVMARLFFAAMLSIAVRGADEGSVVATEALLLAQDADLAYEEASAHGVLAGIDLM
                     RADYAAAVAHCGLGDLRCPDPGTRALCVAFDAAVSADVGQGERAVELAAEAVELADAS
                     ADPIRVVYAHAYAGHALLLQDQPEAARPHVERAIQAASAVLVLQPWPLAMLADIDVRA
                     GRLDAAERSAEQARAMSSTTGIAYQQALALRASALLAAARGDRDRALDELVRALTLAR
                     RTSGEGYTFHWPIAWVLESLARVSAPVDRASSQRWAQALLDHATAVGMHTFIRCGQEL
                     MAGE"
     gene            complement(13964..14236)
                     /locus_tag="PLZ72_04610"
     CDS             complement(13964..14236)
                     /locus_tag="PLZ72_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HNA35616.1"
                     /translation="MPRYVIERNIPGAGTLSESELHDISAKSNEVLASMAPRAQWSHS
                     YVTDDKIYCVYLAENEDAVREHAARGGFPADVVARVHTVIDPISGS"
     gene            complement(14384..16177)
                     /locus_tag="PLZ72_04615"
     CDS             complement(14384..16177)
                     /locus_tag="PLZ72_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNA35617.1"
                     /translation="MDHLPEPLLFPRPRRIDRLDPSHRHVLPLVELPDPTLPAQGFAL
                     TVGDERAELRHADDAGLRYGRQTVQQLRDADGTLPTVSLSDHPDFATRGFMLDVSRDR
                     VPTRETLARLVEVLAACRYNQLQLYVEHAFAYRDHAEVWADASPIDAADLTWLDGVCA
                     SAGIELVANQNCFGHLGPWLALETYRPRAECPDGYEVAPGLRFPPAVLAPTDDNAELV
                     LALVREQSAALRSSTVNVGCDETFELGLGVSRERVERDGRAAVYLEHLHRIVDPLVAE
                     GRTVQFWADIVAHHPEHLDAVPSEGTVALVWNYDGPDAPTVEVPPFVTGILDTLGIDI
                     NADTRFAERLQPFADAGLTCWVAPGTSSWNSIVGRLENARANLTDAAVAGRAAGAAGY
                     LVTDWGDGGHHQPLTVSLAPIALGGALSWGVDAHRDLDVGAVVDEVLLGAPGLGVGSI
                     LERIGGVAERTGLVARNSSPIFSALFPGSFTVTGSGEPDREEVEGVVATLEEVLAELA
                     ALDPTPRFGAELIEELAVAVELARFGAGSLAMRAGADGPVPSDRAEALDGLIERYRRA
                     WLTTSRPGGLDRSAAHLERTRDALRSRPGTP"
     gene            16247..18670
                     /locus_tag="PLZ72_04620"
     CDS             16247..18670
                     /locus_tag="PLZ72_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNA35618.1"
                     /translation="MNGTALDLDDKARLLGGATSWRTHAVEAAALPAVKMSDGPNGVR
                     GETESDGMVPGVVVPVGIALGATWDPDLLGRIGDLLGRESVRKRAHVLLGPTVNLHRT
                     PIGGRVFECYSEDPELTARLAVPYVRAVQAHDVAVTVKHFVANDTEVERGTVDVRVAE
                     APLRELYLRPFEATVIEADAWGIMSAYNRLDGEHCAHHHRLLSEILRDEWGFDGFVVS
                     DWDGAHDTVGAISGGLTVAMPGPETIFGEPLAEAVRDGQASEADVDARVAEVIRLIER
                     TRAVERPADGPQLSVDDPDERELCLTAAAASITLLANDGAALPLAEQSRIAVIGPNAA
                     HTRMMGGGSSSLRPLPHRSIAQAIDDRFGGRVVAHALGADTEKMCPPIDEEQLRRADG
                     SPGLDLSFYAAPDTSGPVAATGVTDSSRHLVVGGTPEMFGRGQFGVTMTGSFIPAVSG
                     PHVFGASIAGKGRVTVGDTVVLDDPARDLPRGDWVFGFGCAEQFATVDLVAGEPVPIR
                     VATSGVRGMAALTLGVRAPRDVELIDEAVAAAAAADVAVVVVGTSDEWETEGVDRTTL
                     ALPGDQDELVRRVAEVASRTVVVLNCGGPVLTPWADQVDAVLWASFAGQETGPAVAAV
                     LAGDRDPGGRLPITWPVRLEDCPAWPHYAPVDGVQTYGEGHMIGYRGHDASGVAPRFA
                     FGHGGSYGSSRWSDASLVREVIAVGEDAEVAVTVESTGDRPVTDVVQVYREEDAAGRP
                     PKALVGFAKAVTEPGRTATVTVRIPAAALRRWDDELDSWRTDVGTHRLLVAASATDVR
                     HTVELTVSG"
     gene            complement(18662..19864)
                     /locus_tag="PLZ72_04625"
     CDS             complement(18662..19864)
                     /locus_tag="PLZ72_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA35619.1"
                     /translation="MAEHRFPPGFLFGASTAAHQIEGANTNSNWWAIEHHPSSHVAEP
                     SGDAADSFHRWGEDLDLVAELGFNAYRFSIEWARIEPAPGELSYAALAHYRAMIDGAL
                     ERGIEPVVTLHHFTEPRWVTDAGSWADAGSVDRFVGYVERAVGILDGVRYVATINEPN
                     ILGVMARVMHLGSGGDDPDRERDIPTAPLPAPHLASEVNVIAAHHAARDAIKAARPDL
                     QVGWTVASQVISAVPGGEDTAAAFADLVEDRYLRESTGDDWVGVQSYTRNVFGPDGFI
                     RDDPEDTRTLTGWEYWPTAVGEALRHTAGIVGDVPMIVTENGIATDDDERRIAYTDGA
                     LSSVRAALDDGLDVRGYLHWSLLDNYEWGSYTPTFGLVAVDRETFRRTVKPSARWLGD
                     IGRGIPST"
     gene            complement(19864..20931)
                     /locus_tag="PLZ72_04630"
     CDS             complement(19864..20931)
                     /locus_tag="PLZ72_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35620.1"
                     /translation="MNTSRRPDRRSRRVPLAVVALAAVALVAVAACASDDDGDSSADA
                     TTTSAEKSESPESSEGAVAEIGTFEVGRDTTTLVDDSRPTKAHGGQAELPSRTMPLEV
                     WYPKAAAGKKGAAGEKDATFPLIVFSHGSTRRAVHYAKTLEAWASAGYVVVGPDFPLS
                     KEGTPGGTDYGGIAEQAGDVSFVIDQVLASATDPGRPWAALVDPDRIGLGGQSFGAIT
                     TVAVAINPCCADDRVDVITEFAGAWADLGSPGPVPAESAPPALLVHGDADPTVSYATG
                     QALFDMYPGTRRLLTLVGSGHDPGYFEGLDEPLDELVTRATVDFYDQYLKDDPEAAAQ
                     LDALVAAAGPQVATLTPPTEG"
     gene            21109..21582
                     /locus_tag="PLZ72_04635"
     CDS             21109..21582
                     /locus_tag="PLZ72_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35621.1"
                     /translation="MDDEVPTTTLELALAWPTRVAREVLLAPWTIQRVRRSLGVLPDE
                     LEALRTTLNDTTQVLEETLGPLDGRLENVAGGVNRLDAAVTTLSGDLMGLLGELERLL
                     PELSGMVNGMDGRVERVELVVSQLSDTLLAVAGAVPGVRRAVRGARALPADSTPT"
     gene            complement(21639..22463)
                     /locus_tag="PLZ72_04640"
     CDS             complement(21639..22463)
                     /locus_tag="PLZ72_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA35622.1"
                     /translation="MSEWETVEGEHWAEYAEHYTRMLAPYGEAVLAAAAPQPGERVID
                     VGCGNGDLSLLAAVAVGPDGVVQGVDLSPAEVAVASRRSAEAGLSAARFAVADAARVE
                     VDAPFDVLVSRFGVMFFDDPTGAFSHLRSLLRPGGRIAFACWQELFANEWMIVPGGAV
                     ASVLGLPGGNSGGPGPFSLADPDLTRQMLTAAGFVDIDVEPVAAALWMGADADAAVEF
                     MTRTGMGRILVGDAAPEQRAEAIERARDALRPHETDSGVMLDGAAWVVTATNPPTP"
     gene            complement(22512..>23482)
                     /locus_tag="PLZ72_04645"
     CDS             complement(22512..>23482)
                     /locus_tag="PLZ72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA35623.1"
                     /translation="SQGVTSISAMCQSADEGPAGPAGAMEALGWAALSGDLPFDIQTI
                     LITPEPSEVFDLRDPSLHDPARGRRLDAVKLFLDGTLGGRTACMHGPFADHPHEHGMR
                     TTDDDEAYRRMEAAHLAGLQICVHAIGDAANRSAAELFDRLLVRHPGRHRHRVEHASV
                     LDDPTIELFARHGITAVVQPINLRSESHWLARRVGLDRLGRTYAFASMTSGGVSIAGS
                     SDAPIERTDVLAAMAAAVDRGGQAPEQALTRSEALALFTTGGSFARGTEDELGSLATG
                     RRADLVVLDADPTDERTDLDALGVRSTVIAGEVRYRADRSAGFVPR"
BASE COUNT         3194 a         8651 c         8360 g         3177 t
ORIGIN      
        1 gacgcggccg acctgctcgc cggcgcgcag cgcccgttcc tgttcgccgg caagggcgtg
       61 ctgtgggccg aggcgtccgc cgccttcgtc gccctgggcg accacctggg cgcggcgatg
      121 agcaccacgc tcggcgcccg gggcgccgtg cccgaggacc acccccacta cttcaccatc
      181 gcggatggca acaccctgcg agcggttcgg ggcgacgccg acgtggtgct ggtcgtcggt
      241 gcccggctcg gcgagtacga cggctggggc gtgccgccgg cgtggggtga ctccgaggac
      301 caacgcacca tccagatcga ctccgacccg atgtcgatcg ggctgaaccg cccggtcgac
      361 ctggccgtgg tggcagacgc ccgcggcgct ctcgaggcgc tgctcgccgc ggtcgccgag
      421 cgcgtcgagg cccggcccgg cctggagggg gcggagcgct acgccgcgat ccacgagcag
      481 tcggtcgccg aggccacccc gcacgtcctc gccgagccga ccgtcggggt gaacccggga
      541 cagatggtca tggcgctgcg cgagctgttc ccccgcgacg cggtcaccgt ggccgacggc
      601 ggcaacacca cgttgtgggc cgtcgcgctc aacccggtct acgtcccgga ctcgttcctg
      661 tactcggtga agatgggcta cctgggaacc ggtctgccgt tcgcgatcgg ggccaagctc
      721 gcggcgcccg aacgcgacgt gtacctgatc agcggtgacg gcgcgttcgg cttcaacgcc
      781 atggagctcg agaccgcccg ccgcgtcggc gccccggtga tctgcgtggt cgcggtcgac
      841 ggcggatggg gcatggagcg caccgctcac cggttcatgg gcgtgcccga cgacgaggcc
      901 cacggcaccg acctgtcacc cgaggtccgc tacgacgagc tggcccgggc gttgggctgc
      961 cacggcgagc acgtcacggc catggacgag ctcgccccgg cgatcgagcg ggcccggtcc
     1021 tcgggtctac ccgcggtgct ccacgtcgag gtcgatcccg acatcaacgc caacccgatc
     1081 ggctacgagc agttccgctc cgcccgcacc tactgacgtg gacgatccgc ccgcctcggt
     1141 gttcgtgacc ggcgccaacg gcttcatggg ccgggcgctg gccgagcggt accgcagcgc
     1201 gggggtcgac gtgtccggtg tcgacgtcac ggccgatccc gaccgcggcg tcatcgccgg
     1261 cgacgtcctc gacccgtccg gttgggccga cgccatcgcc ccgggtgcgg tggtgatcca
     1321 caccgccgcg atcgtgtcca acaacatcgc caccgaacgg gcgtgggagg tcaacgtcgt
     1381 cggcacccag cgggtgctcg acgccgcgac cggcgcgggc gccgagcgct tcgtgcagat
     1441 ctcgaccatg ggcgtgacgc gcttcgccca cacccacgcc gccgcggcac ggcgaatcct
     1501 gcccggccgg gagctcgacg agcggtggcc gttgatgccg accggcaacc cctacaccga
     1561 caccaagatc acggccgagc acctggtgct ggccgcgcac gccgccggcc gccaggcctg
     1621 cacgatcatc cgcccggccg acgtgtacgg acccggctgt cgaccgtggg tcctggagcc
     1681 gctcgaggcg atccgctcgg gtcggatgct gctgccggcc cggggtcggg gcctcttcac
     1741 gccgatccac atcgacgacc tcctcgacgg cgtcgtggcg gccgccgctc ggcccgccgc
     1801 ggccgggcag atcttccacc tgggcggcga ggagccggtg accaccctcg agtacttcgg
     1861 ccacctggcc cggatgatcg gccgccccgc accccgcacc gcaccgaccg cggtcgcggt
     1921 cgccgcggcc gaggcgaccc gctgggtggc cgtcgcccgt cgacgccaca ccgaggtggg
     1981 ccgcggcgtc atggcgatgc tggccaagac ccgtgccgtg tccaacgaga aggcccaccg
     2041 ggatctcgac tggtggcccc gggtctcgct gagcgacggg atggcggacg tcgagcggtg
     2101 gctccgctcg gaggggctgc tcgacgacgc acgctgatcc cggccggtga tcccgaccgt
     2161 cgggacccac ccgcgacgcg caggtcgcgg tcggagcacg gcactacggt gcggccatgt
     2221 cagcaccagg acagacagga tcggacggga ccgcggtcgg cgcgggcacg taccgcgccg
     2281 agatccgctg gaccacccac ggcgtcgcgc acatccgggg cgagtcgctg cccgacgtgg
     2341 cgttcggcca ggcgtatgcc atcgccggtc accacctgcc gacgatcgcg gaccagctgc
     2401 tcaagacccg cagcgaacgg gcccgccact tcggccgggg cgacaacgac tgccacgtca
     2461 actccgactt cgggtacctg gccatggaca tgaccgcctg ggcgcagcgg atgctggcca
     2521 cccaaccgcc gtcggtggtg gaggtggtcg aggcgtacgc cgccggactc aaccgctggt
     2581 tggccgagca cggcaccgcg gacctgcccg agtggtgccg ctccgccgag tggatccgcc
     2641 ccgtcgacgc cgtcgacctg ttccgcctct acgccgacat gatgctgatg gcatcggggc
     2701 gcaacgcggc ggagttcgtg ggagccgccc agccgcccgg atcgccgccg gtcgcccatc
     2761 cggaacggcc gctggttccc gacgacgggc ccggctccaa cggctgggcg ctcggcgggg
     2821 acgtcaccgc ggatggtcgc ggcgcggtcg tcgccaaccc gcacttcccg tggtacggcg
     2881 acggacggtt ctgggagtgc cacctgacgg tgcccggcga gctcgatgtc tacggcgtgt
     2941 gcctggtcgg agcgccgggc atccagatcg ggttcaaccg cgacgtcgcg tggacccaca
     3001 ccttctcccg gggccagcgg ttcaacttct actcgctgca gctcgacccg tcggatccga
     3061 cccggtaccg ctacggcgac gagatgcggt cgatgacgcc gcgggtcgaa gcgatcgagg
     3121 tgctcagctc cgacggatcg ctcgagaagg tcgaacggac cctgtggtcg tcgcactacg
     3181 gaccgatgct cgatgtcccg ctgctcggct ggagcgacgc cctggcgttc tcgttggccg
     3241 acgtcaacca cggcaacgac cggatcctgg cccagtacct gtcgatggac gtcgccaccg
     3301 acgtcgcctc gctgcgcgac tcggtgcgca cccaccaggg actgccgtgg gtgaacgtca
     3361 tcgccgcgga ccgtcacggc gacgtctggt acgcggaccc gtcgccggca ccccgcctca
     3421 gcgccgaggc cgaagccgcg ttcgaccggg ccgtcgagac cgacccgatc accatgctct
     3481 tctacagcca gcgcgtcgcc atcctcgacg gctcggaccc ggccaacgag tgggtcgacg
     3541 acccccgcag cgcgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg
     3661 aggacctcga gcgggcgctg ctcgacaacc ggtcgctgct cgccgagcag ctcctcgacg
     3721 acgtgatcga ccggtgccga cgatccggtg acgccacgct ggccgacgcg gcggaggtgc
     3781 tgtcgggctg ggaccgggcc ttcgacgtcg gctcacgcgg cgcggtgctg tggcgggagt
     3841 tcctggccac cttcagcgag gacgacctcc gcgggggcgg ctcgctgttc gcggtgccct
     3901 tcgacgtggc cgacccggtg cacaccccac gcgatttggc cccggcgccg gagtccgggc
     3961 ccgacccggt ccacaccgcc atgtggagcg ctctcgacgt gctcgcgtcg gccgggatcg
     4021 ccgccgacgt cgcgctcggc gacgtgcagt tcatcgaccg ctccggcgaa cggatcccca
     4081 tgcacggcgc caacgaggtg gagggcatca tcaacgtcgt cgccccggtc ggggccttcg
     4141 gcggcagcga cctggagccc cgcccgtcgt ccgggtcccc gatcccgggg cggaccgagc
     4201 ggaccggtct gtgctccacc ggctatccga tcacctatgg cgcctcgacc ctgatggtca
     4261 tcggctggga cgacgacggc ccggtggcgc ggggactgct gacctacggc cagtcgggcg
     4321 acgcccgatc cgagcaccac gtggaccaga tgcgcgagtt ctcggccaaa cggctccggc
     4381 cactctgctt ccacgacgcc gacatcgacg ccgatccgca gctgcgggtc gaggtcgtcg
     4441 agggctgacc accccgtcgg tccgatcacc ggagacgtcg gggcgggctc cacccggccc
     4501 gccccgacgt cgagctcacc cgcggtggaa cgtggtccgc cagtcgccgc cgtcgagctg
     4561 atcggcgacg taggcgaacc cctgctcccc gagcacccct tcgaggggta cgggctcgaa
     4621 cggggcgtag acgaccaggg cctcgccgtc gcccacctcg gccgccgcgg ccatgatctc
     4681 gccgaagggc tctccgccgg ctgcgagggt gggccgggca tcgaccacac gggcgaccga
     4741 ggtgatgtcg atcgcccact cctcgggccc gctctgcacg tactcccagc ggaactggtg
     4801 cgggcgggtc gcctcgagct ggtggcgcag cggggccggg tcgtggtcgt tgaccagccg
     4861 gagggtgtca ccggtgccga gcgcgtcgag cgtctcgaag atcgtctggt gccggaccgg
     4921 cgggggaagg ggccgtacgt cgatggtgtg catcacgcct cctgggatcg ggcggctccg
     4981 gttccggcac cgcgtcccgg caccaccggc ggggtgaccg gagaccggtg cgtcgatctg
     5041 gtcggtcgcc ggagcgccgg atattgttct agccgacact agaaaatctt tcgggagtcg
     5101 tccagtgacg gtccaacccc ccaccgcccc gcagatcccg gcgacggccc cggcgtccgc
     5161 gcccaccgct gcgccgttcg ttcccggcgc caccgcggcg gtcccgccgg cgtcggtgcc
     5221 gctcgggttc ctcgccgcgg ccggcgtcgg cctcgtcgcc ttcgggctcg cggcgtggtt
     5281 ctccgccgat cgcatcgtcg acatgccgac ccatccgggc tcggtggcag cggtgcacgt
     5341 cggcatgctc gccttcctga ccaccgcggt gctgggcgcg gcccaccagt tctcacccgt
     5401 cgtcggccgg cgccccctgc gctcggtgcc ggccgcccgg gtgaccctgg tcggcatggt
     5461 cgccaccgcc tggatgctgc cgtcggggtt cgcccacggc cccgaggcgc tcgtgctggc
     5521 cggcgggatc accggagcgg cgacggtcct cttggccgcg tggaacctct cgggaccgct
     5581 cagcacccgc gacggcggtg ttccgctcgt ggggctccgc gtgtcgatcg cctacctggt
     5641 gctgaccgtc gccttcggcg tcacctacgc catcgaccgc cagaccctct ggttcccgct
     5701 gctgcccaac cgggtcctgg cccacgccca cctcgggctc atcggctggt tgggcatcac
     5761 ctacgtgtcg gtggccgaga agctctggcc gatgttcctg ctcgcccatc ggccccgggc
     5821 ccgggccggg gcctgggcgg tcggcctgct cggcagcggg gtcggggggc tggctgtcgg
     5881 cctcctgctg gagtccccga tcgtggtcgc cctcggcggc accgcggtcg tcgtcgggtt
     5941 cggctgccac atcgcgtcgc tcgtcggtgt cgtccgggcc cggcggcgac ctctggagct
     6001 gctgcaccgg ttcctgttca cctcgaccgc gttcctggtc gtcgccatcg ccctcggcgt
     6061 cgccgccgcg ctggcccccg tcgactacgg cacccgcagc cgactggtgt cggccgaggt
     6121 cgcgtcgctc gcggcgtggc tcggactggc ggtcatcggc cacgtgcaca agatcgtccc
     6181 gttcatcggc tactcggccc tgcgtgcccg cggcgtgacc cacgccccga gcggcaagcc
     6241 gctgatgttc gccgacctct tccacccggg gatcgcccgc gccaccctgg tcgcctccgg
     6301 tgcggggttc gccctggccg tcatcgggat cctcgtcggg tcctcgacgg tgatcgccct
     6361 cggcggcgtc ggcatcgcca ccgccggcgt gctcgtcacc gccaacctgg tgtcgggtcc
     6421 gcgtcgggtc acccgcgcct cggcccgatc cgcccccgcg accgccgcgt gagcgccgac
     6481 gtccaccggg cgattgtcgc cggcccgacc gccgtgatta ccctcgaacg ctcatcgatc
     6541 tgggaggcgt gcccgtgacg attgacgcaa agctgcagca ggtctggacc gacgtcgtgt
     6601 cccggaaccc ggccgaaccc gagttccacc aggccgtcca cgaggtcctc acctcgatcg
     6661 gaccggtcct ggccaagcac cccgagctgg cgcgactgaa gtcgatcgag cgcatctgcg
     6721 agcccgagcg tcagatcatc ttccgggtcc cgtggatgga cgacgacggc gagatccaga
     6781 tcaaccgggg cttccgcgtc gagtccaaca gcgccctcgg cccctacaag ggcgggttgc
     6841 gcttccaccc gtcggtcaac ctggggatca tcaagttcct cggcttcgag cagacgttca
     6901 agaacgcgct gacccagatg ccgatcggcg gcggcaaggg cggcgccgac ttcgacccca
     6961 agggccgcag cgacggcgag atcatgcggt tctgccagag cttcatgacc gagctgtacc
     7021 gccacctcgg cgagtacacc gacgtccccg ccggcgacat cggcgtcggc gcccgcgaga
     7081 tcggcttcct gttcggccag tacaagcgca tcaccaaccg ctacgagtcc ggcgtgctga
     7141 ccggcaaggg gatcacgtgg ggcggcgccc tggtgcgcac cgaggccacc ggctacggcg
     7201 ccacgttctt cgccgaggag atgctgaagg tccgcggcac cagcctggac ggccggacgt
     7261 gcgtggtctc cggatcgggc aacgtcgccc tgtacgcgat cgagaagctc ggtcagctgg
     7321 gcgccacggt ggtggcctgc tcggactccg agggctacgt ggtcgacaag tcgggactgg
     7381 acctggagct gctcaaggag atcaagctca acgcccgggg ccgggtcagc gagtacacgc
     7441 agcagcaccc cgacgcgcag ttcgtgaccg acggcagcat ctgggacgtc ccgtgcgacg
     7501 tcgcgcttcc gtgcgccacc cagaacgagc tcaccggcgc cgacgcggcc accctgatcc
     7561 gcaacggcgt catcgccgtg gtcgaagggg cgaacatgcc cgccaccccc gacgccgtgt
     7621 ccgcgttcct cgaggcgggc gtcgcgttcg ggcccggcaa ggccgccaac gccggcggcg
     7681 tcgccacctc ggcactcgag atgcagcaga acgccagccg cgacagctgg accttcgagc
     7741 acaccgagga gcggctcgcc cggatcatgg ccggcatcca cgacgcctgc tacgagaccg
     7801 ccgaggagta cggcgctccc ggcaactacg tcgtcggggc caacatcgcc gggttcctgc
     7861 gggtcagcca ggccatgctc gccctcggcc tcatctgagc cctggaggac tcgaccgggg
     7921 ccggcggcca tccggtcagg cccgatccgg tcagggccgt cgcaccgaca ggtccaggta
     7981 cgacggctgc gccacgctgt ggtgcaggtc ctggtgtccc ccggccagtc cggccatgtc
     8041 ggccaggggc aggaaatggg ggaagtccgc ggtgctgacc cgcagccgca gtcggtgccc
     8101 ggccggcacg gtcacgaacg cgggtcggac ctcgacgggg tagcgcaccg cctcaccgac
     8161 cggcaccggc tgtcgcgccg cagcggtcag gagctgatgc ggccgcacca gccggccctg
     8221 ggcgtcggtc cagctccggg agtcgtcgag cgcccgcatc gacccgagga tcgctccgcc
     8281 ggtcaggccc atcgacgtgc cgtccggggc cacgtcctcg acggtgacgc tgaacagcac
     8341 gtcgggactg ttcgaggtgg cggccagcga cgcaaggacc gggccggcga gcgtcagggg
     8401 ttcggccaac gggtccgtgc tgtaggtgac ctcgttggga acctcgggcg cggtcggccg
     8461 cggcaccagg cagtccttgg cgcccagacc ggcggcgaac tgcaccgtcg acgcggtgca
     8521 ggggtcgccc agcgggaagt agtccacgcc gtccgagggc tcgtcggttc ctggcggctc
     8581 agggtcgagt cggccgtcgg cccgcaggta cagccgctcc agcggcgcgt cggcgagcgg
     8641 gtaggtcgcg gcgtcgtagg tggttccgcc gggctcgatc acccgcaggg gtgcggccgg
     8701 gtcgccgtcg gctgactgtt caccgtccgc ctgccgttcg tcgtgcggta cgtccttcag
     8761 ccaccggtcg aaccaggcga gctgcaccgc cgacaggtcg atcccctcgc cgatcgtgcc
     8821 gtgggtccac ggcccggtca gcagccggaa ccggtcgctg accggctggc cggccagcat
     8881 cggcgcctcg accggtcggc cggccgccgc gttctgcagg ccgctgtaga gcaggggctc
     8941 gccccgctgg aacacgtcgt agagcccacc gaccaggtgc acggccacgc cgttgtcggc
     9001 gatgcgctgc agcacccgct ccgggcggcg aagctgccac cagtcgccgt cgtatcggcg
     9061 cggaccgttg tcgaacatgt cttgggcgat caccccctcg aagcgaccca tctgctcgag
     9121 gttcgccgcc gcgtgctgca tcgcgagcgc cgggtcgtcc caggcctcgg cgaacggggt
     9181 gacgctgcgg gtgatgccgt agccgaacag caggccgagc ggtgacagga cgttgagcgc
     9241 cccaccgctg gtgaacaggt cgcggtaggg gtcgacggtg gcggccatcg ggaagatggc
     9301 cttcaacggc gagtcgggtc cgaccgccgc cgcggtgaac agctgcacga tggccatgta
     9361 ggacagcccg agcatcccga ctcggcccgt cgagcggggc agcgcggcgg cccagcggac
     9421 gatctgggcc cccgcggtcg cctcggcggg gtcgaacagg tcgaaggacc cgtcggagat
     9481 gccggtgccg gggacgtcga cggccagggc gaggtagccg tggcggacca gctgcaggtt
     9541 gacgccgttg cctccgggga tcgacgcccc cgccgctccg gacttgccgt agggcgtcat
     9601 cccgacgatc accgggaacg ggcccgccgc ggccgtgccg gtgccggggt cggtcggttc
     9661 gtacagatcc gcacgcagga cccggccgtc gtcgagaggg atggtcaggt tgcgggtgac
     9721 ggtgacgccg tagggcccgt cggtgccggt caggtccagc ggcgcgggcg gagagcaggc
     9781 gaccgctgtg gccaccatca ccagcgcgac caggccaccc acgacccgcc ccctcgacac
     9841 cccgcctctc gacaccccgc cgctcgacag cacggccgcc atggtcgaag cacccatcgc
     9901 cgattccccc cggtcctcgc tccgccgatc cagaaacatt gttgttcgcc gatgctatcg
     9961 tcgggtgaca tggacgagca acgagcccgc acccacccgt cgctggccgg ccgtcgggtc
    10021 ctgatcaccg gcgccgcccg cggcatcggc gccgcgctgg cccaacgcct gcacgaccgg
    10081 ggcgcccgcg tcgcgctcgc cggcctggaa cccgatcagc tgcgggccac ggccgacggc
    10141 ctcggtgcgc cgttctggga ctgcgacgtc accgaccgca gccgcgtcgc ccgggtcgtc
    10201 gacgaggccg ccgccgagct gggcgggctc gacgtcgtgg tcgccaacgc cggcatcgcc
    10261 gcgcagctcc cgctggtcga cggcgatccc accatcttcg agaagaccct ggcggtgaac
    10321 accctcggca cctactacac gttgcgcgag gccggacgac acgtcgcgca ccccggcggc
    10381 tacgcggtgg cggtgtcgtc gctggccgcc gcggtgcacg tcccgctgat gggcgcctac
    10441 agcgcgtcga aggccgccgt cgaggctctc ggcaacaccc tccgccagga gctgcggccc
    10501 tccggcgccc gggtcggcgt cgcctacttc gccgagctcg acaccgacat gacctcccgc
    10561 ggcttcggca ccgaggccgc cgccgcgttc ctcgacggac ggagcctcac ccgggtcacc
    10621 ccgctggtga agggcatcga cgcgctggag cggggcatca cccgccgctc ccggatcatc
    10681 tgcgcgccct ggtacgtcgc gccgatcctg ccgacccgtc gggtcgccca ggccgtcgtc
    10741 gagctcgcca cccggcgccg actcgcaccg gtgctcgaca tcgcacgcct ggaacgggtc
    10801 gagctcacca cgccgcagcc cgagcgcagc ccggagatgc cggcgcagga ggaccggccg
    10861 tgagcgacgc cgtcacaccc accgactccc ggaccgcggg accgaccacc ggcccccggg
    10921 tctcaccggg tgaccgccgc caggtcggtc ggctcaacca cgcgatcgcc tcggccctgg
    10981 gccgcgccgc gggcaccgca gcaccgaacc tgttcctgac cctcgggcgc caccgacgcc
    11041 tgttccgcgg ctggctgcac ttcgccggga cgctgatgcc cggcggacgc ctgtcgcggc
    11101 gcgagtccga gacggtgatc ctgcgcgtcg cgaaccgccg gcaatgcgac tacgagcggg
    11161 agcaccaccg gcgactcgga ctccgcgccg ggctcaccgt ggacgacgtc gacgccctcg
    11221 gcgcgcccga cactgccacc gaccctcgct ggagcgcacg ccaggccgcg atgatcgccg
    11281 cggtcgacca gctcctcgag caccgcgacc tcgacgacca ggcgtgggcg tcgctgcggc
    11341 gtcggctcga cgaccgcgag ctgatcgagc tggtgatgct ggtcggccac tacgacatgc
    11401 tggccaccgc gatcacgacg ctgcggatcc agcccgacgg accccggaca cggtgaccga
    11461 cgcagtggcc gcctcgaccg acgccacgcg acgcctcccc cgcggccgcc acgacctgac
    11521 ccgcgagcag atcgaagagg accagcgcct gcggatcctg ctcggcttcg ccggcgccat
    11581 ggccagcaac ggctacgccc gcaccccgat cgccgcggtg atcgcccacg cgggcgtgtc
    11641 ccgcgagacc tactaccggt tgttctccga caagctcgac ggcttcctgg ccgcgttcga
    11701 cctggtgagc accgtcctgc tggccgagct cgaccgggcc gtcgagggac cgggcacacc
    11761 gctcgatcgg atcgacgacg gcgtgcgccg gtacctggag gtgctggccg agcaccgcgg
    11821 cgaggcccgg ctgttcctgg tcgaggcgtt cgcagcgggg cccgtcgcga tcgagcgtcg
    11881 tgccgaggtg caggagcgga tcgcggcgcg catcgccgag gtgctggagg tcggctccga
    11941 gtccggccgg gtcacctgcc ggatgctcgt cgccgcggtc agcgccctga tcgtgacgcc
    12001 gctggtcgcc gacgaccacg acgcgatcct ggccctcggc ccgctcatca gcgccgagtt
    12061 gcgccgctac cacgccgccg gcctcctcag ctgaacctga acctgcccgc cctttccccc
    12121 tcccctcccc gttctgttcg gtggatcaca acacgagtgt tgtgatccac cgaacagaac
    12181 ggggagcggg ggtcagtggg tggcggtcac tcccccgcca tcagctcctg cccgcatcgg
    12241 atgaaggtgt gcatgccgac cgcggtcgcg tggtccagca acgcctgggc ccaccgctgc
    12301 gacgacgccc gatcgacggg tgcgctgacg cgcgccagcg actcgaggac ccacgcgatc
    12361 ggccagtgga acgtgtagcc ctcccccgag gtccgtcgtg ccagggtgag ggcacggacc
    12421 aactcgtcga gcgcccggtc ccggtccccg cgcgccgcag ccagcagcgc cgacgcgcga
    12481 agtgcgagcg cctgctgata tgcgatcccg gtcgtcgacg acatcgcccg ggcctgctcg
    12541 gcggaccgct ccgccgcgtc gagccgcccg gcccggacgt cgatgtccgc gagcatcgcc
    12601 aacggccacg gctgcaggac gaggacggcg gacgccgcct ggatcgcgcg ttcgacgtgg
    12661 ggccgggcag cttctggttg gtcctgcagc aggagcgcgt gtccggcata ggcgtgggcg
    12721 tacacgacgc gtatcggatc cgcgctcgca tcggcgagct cgacggcttc cgccgcgagc
    12781 tcgacggccc tctcgccctg gccgacatcg gcggagaccg cggcgtcgaa cgccacgcac
    12841 aacgcccggg tccccgggtc ggggcatcgg aggtcgccca gaccgcagtg agccaccgcc
    12901 gcggcgtagt cggccctcat caggtcgatg ccggcaagga cgccgtgggc cgacgcctcc
    12961 tcgtaggcca ggtcggcgtc ctgggccaga agaagtgcct cggtggccac caccgagcct
    13021 tcgtcagccc ctcgcacggc gatgctgagc atcgccgcga agaacaggcg ggccatgacc
    13081 cgcagcccga ggtcgtgctc gcggtccgcc atgctcgacg cgtcacggcc cagatcgatg
    13141 gcgtgatcca ccgagccggc caggaacgac tgccacgcga tcgccaaccg cgcctcgacc
    13201 gtggcgacat cgaccggttg acgacgttcc gcgacctcgg acgcgaagtg caccgccgcg
    13261 accaccgccc cacccggctc gaccgacagc tcacggcgca gcatctcccg acaccgctcg
    13321 tactgggaac gggccatgcc gacgtcgccg gccacggcat gggcccggat gagcagggcg
    13381 tggctgctct cgtccaacgg atcggtcgcg accagggccg tggcgaggtc caacgccgtc
    13441 tcgtgatcac cggacgccag gccgcgcagc accgcctcac gccgccgtga catctgcagt
    13501 cgcgacaggc gggcacgctc cccggcgagc cagagctcga accgggggct gtcggggaac
    13561 gacaaccccg cgagcagcag accgtccggc gccgggtcgg tgggcggacc gtgggcggac
    13621 aggacgtcgc ggacgtcgat gcgggcgtcg gacagatcca gaccgatcgg gtcgcccttc
    13681 acgacgtcgg gacggtcgag gagtcgacgc agcgcggcca ggttccagcg caacgctccg
    13741 agcggatccg acgccccgct gaacagcagc tccgccagct ccgcccgggg tcggggacga
    13801 tcctccgcga cgaggtaggc gagcagggcc cacggcttgg cgcccttcgg cggcggcacc
    13861 tcggcaccgt cgacgagcac ggccggacgg cccagcagtc gaacttcgag ctgcgccacg
    13921 cgtccagctt cgcgcccgtc gacgtcgagg tggcgagaac ctgtcaggat ccgctgatcg
    13981 ggtcgatcac ggtgtggacc cgggcgacga cgtcggccgg gaaccccccg cgggctgcgt
    14041 gctcccgcac cgcgtcctcg ttctcggcga ggtacacgca atagatcttg tcgtcggtga
    14101 cgtagctgtg gctccactgc gcccgcggcg ccatcgacgc gagcacctcg ttcgatttcg
    14161 cgctgatgtc gtgcagctcg gattcgctga gggttccggc gcccgggatg ttccgttcga
    14221 tcacatagcg aggcatggtt cgactccttc ggtggtggtc ggtcaaaccc accgtcgcgg
    14281 accaccgtgt gaaccatcgt gtgaaccccg cgctttcggg ctctgttgtc gttttcgtga
    14341 ccgttcgcgc caccagtggc gcgaacggtc acgagaatcg tgctcagggg gtgccgggac
    14401 gggagcggag ggcgtcgcgg gtgcgttcca ggtgagccgc ggagcggtcc agacctccgg
    14461 gtcgactggt ggtgagccac gcccgccggt accgctcgat cagaccgtcg agcgcctcgg
    14521 cgcgatccga cgggaccgga ccgtcggccc cggcccgcat cgccagcgac ccggcgccga
    14581 agcgggccag ctcgacggcg acggccagct cctcgatcag ctccgcaccg aagcggggcg
    14641 tggggtcgag cgccgccaac tcggcgagca cctcctccaa cgtggcgacc accccctcga
    14701 cctcctcccg gtcgggctcg ccgctgccgg tgacggtgaa gctgccgggg aacagggccg
    14761 agaagatcgg gctcgagttg cgggccacca ggccggtgcg ttcggccact cctccgattc
    14821 gctcgaggat cgaccccaca cccaggcccg gcgccccgag cagcacctcg tccacgaccg
    14881 ctcccacatc gagatcgcgg tgggcgtcga cgccccacga cagcgcgccg ccgagggcga
    14941 tcggcgccag gctcaccgtc aacggctggt ggtggccgcc gtcgccccag tccgtcacca
    15001 ggtaccccgc cgccccggcg gcacgacctg ccaccgccgc gtccgtgagg ttggcgcggg
    15061 cgttctccaa acggccgacg atcgagttcc acgacgacgt ccccggcgcc acccagcacg
    15121 tgagccccgc gtcggcgaac ggctggagcc gctcagcgaa gcgggtgtcg gcgttgatgt
    15181 cgatcccgag cgtgtcgagg atcccggtga cgaacggcgg cacctcgacg gtcggggcgt
    15241 cgggcccgtc gtagttccac accagggcga cggtgccctc ggagggaacg gcgtcgaggt
    15301 gctcggggtg gtgagcgacg atgtcggccc agaactgcac ggtgcgcccc tcggccacca
    15361 gcgggtcgac gatgcggtgc aggtgctcca ggtagaccgc agcccgaccg tcacgctcga
    15421 cgcgttcgcg gctgacgccc aggcccagct cgaaggtctc gtcgcagccg acgttgaccg
    15481 tcgagctcct cagcgcggcg gactgctcgc gcaccagcgc caggacgagc tcggcgttgt
    15541 cgtcggtcgg ggcgagcacc gcgggcggga accggagacc gggagcgacc tcgtagccgt
    15601 cggggcactc ggcccgtggc cggtaggtct cgagcgccag ccacggaccc aggtgaccga
    15661 agcagttctg gttggcgacc agctcgatgc cggccgacgc acacaccccg tcgagccagg
    15721 tcaggtccgc ggcatcgatc ggcgaagcgt cggcccacac ctcagcgtgg tcccgatagg
    15781 cgaaggcgtg ctcgacgtag agctgcagct ggttgtagcg gcaggccgcc agcacctcga
    15841 cgaggcgtgc gagggtctcg cgggtcggga cccggtcccg gctgacgtcg agcatgaacc
    15901 cccgggtggc gaagtcgggg tggtcggaga ggctcaccgt gggcagggtc ccgtcggcgt
    15961 cgcggagctg ctgcaccgtc tgtcggccgt agcggagccc ggcgtcgtcc gcgtggcgca
    16021 gctccgcgcg ctcatccccg acggtgaggg cgaacccctg ggccggcagc gtcgggtccg
    16081 ggagctccac caacggcagg acgtgacggt gcgacggatc gagccggtcg atccgtcggg
    16141 gccgggggaa caggagcggt tctggtagat gatccacggc tcgacctccg acgggcaccc
    16201 tagggtcgac ccgtacgcca cgccgaccga acccggagca ccagccgtga acggaaccgc
    16261 cctcgacctc gacgacaagg cccggctgct cggcggcgcc acctcgtggc gcacgcacgc
    16321 cgtcgaggcg gccgcgctgc cggcggtcaa gatgtccgac gggcccaacg gggtgcgcgg
    16381 cgagaccgag agcgacggga tggtccccgg cgtggtcgtc ccggtcggca tcgccctggg
    16441 cgccacctgg gaccccgacc tgctcggccg catcggcgac ctgctcggtc gcgagtcggt
    16501 gcgcaagcgg gcccatgtcc tgctcggccc gacggtcaac ctgcaccgca ccccgatcgg
    16561 cggccgggtc ttcgagtgct actccgagga ccccgagctg accgcccggc tggccgttcc
    16621 ctacgtgcgg gcggtgcagg cccacgacgt ggcggtgacc gtcaagcact tcgtcgccaa
    16681 cgacaccgag gtcgagcgcg gcaccgtcga cgtgcgcgtc gccgaggcgc cgctgcgcga
    16741 gctgtacctg cgcccgttcg aggcgaccgt catcgaggcc gacgcctggg ggatcatgtc
    16801 ggcctacaac cgcctggacg gggagcactg cgcgcaccac caccggctgc tgagcgagat
    16861 cctgcgcgac gagtggggct tcgacggctt cgtcgtgtcg gactgggacg gagcgcacga
    16921 caccgtcggg gccatctccg gcggcctcac cgtcgccatg cccggccccg agacgatctt
    16981 cggcgagccg ttggccgagg cggtgcgcga cggccaggca tccgaggccg acgtcgacgc
    17041 ccgcgtcgcc gaggtcatcc ggctgatcga gcgcacccgt gccgtcgaac gcccggcgga
    17101 cgggccgcag ctcagcgtgg acgaccccga cgagcgggag ctgtgcctga ccgcggcggc
    17161 cgcctcgatc accctgctcg ccaacgacgg cgcagcgctg cccctcgcgg agcagtcacg
    17221 gatcgcggtg atcgggccga acgccgcgca cacccgcatg atgggaggcg gcagctcgtc
    17281 gctgcgtccg ttgccgcacc gcagcatcgc ccaggccatc gacgaccgct tcggcggacg
    17341 ggtcgtcgcc cacgcgctgg gggccgacac cgagaagatg tgtccgccga tcgacgagga
    17401 gcagctgcgc cgggccgacg gatcaccggg gctcgacctg tcgttctacg ccgcgcccga
    17461 caccagcggt ccggtggcgg ccaccggggt caccgactcc agccgccacc tggtcgtcgg
    17521 gggcaccccc gagatgttcg gccgggggca gttcggcgtc acgatgaccg gctcgttcat
    17581 cccggcggtg tcgggcccgc acgtcttcgg cgcctcgatc gccggcaagg gtcgtgtgac
    17641 cgtcggagac accgtcgtgc tcgacgaccc cgcccgcgac ctgccccgag gcgactgggt
    17701 gttcggcttc ggctgcgccg agcagttcgc caccgtcgac ctcgtggccg gcgaaccggt
    17761 gccgatccgg gtggcgacca gcggcgtgcg gggcatggcc gcgctcaccc tgggggtccg
    17821 agcgccccgc gacgtcgagc tgatcgacga ggcggtcgcc gccgcggccg cagcagacgt
    17881 ggccgtcgtc gtggtcggca ccagcgacga gtgggagacc gagggcgtgg accggaccac
    17941 cctggcgctg ccgggcgacc aggacgagct ggtccgccgc gtcgccgagg tcgcgtcccg
    18001 gaccgtcgtg gtgctcaact gcggcgggcc ggtgctcacg ccgtgggccg atcaggtcga
    18061 cgccgtgctg tgggcgtcgt tcgccggaca ggagaccgga cccgcggtcg ccgcggtgct
    18121 ggccggcgac cgcgaccccg gagggcggct gccgatcacc tggccggtcc gcctcgagga
    18181 ctgcccggcg tggccgcact acgcgccggt cgacggggtg cagacctacg gggaggggca
    18241 catgatcggc taccggggcc acgacgcgtc cggggtcgcg ccccggttcg ctttcggaca
    18301 cggcggctcc tacggatcgt cacggtggag cgacgcgtct ctcgtccggg aggtgatcgc
    18361 cgtcggcgag gacgccgagg tggccgtcac cgtcgagtcg accggcgacc ggcccgtgac
    18421 cgacgtcgtg caggtctacc gggaagaaga cgccgccggt cgaccgccca aggccctggt
    18481 cggcttcgcc aaggcggtca ccgagccggg ccgcacggcc acggtgacgg tccggatccc
    18541 cgccgctgcc ctgcgccgct gggacgacga gctcgacagt tggaggaccg acgtcggcac
    18601 acaccggctg ctcgtggccg cctcggcgac cgacgtgcgc cacaccgtcg agctgaccgt
    18661 ctcaggttga ggggatcccc cgaccgatgt cgcccaacca gcgggcgctc ggcttgaccg
    18721 tgcgccggaa cgtctcgcgg tcgaccgcga ccagtccgaa cgtcggcgtg taggaccccc
    18781 actcgtagtt gtcgagcagg ctccagtgga ggtagccgcg gacgtcgagc ccgtcgtcga
    18841 gcgcggcgcg caccgacgac agggccccgt cggtgtaggc gatgcggcgc tcgtcgtcgt
    18901 cggtggcgat gccgttctcg gtgacgatca tcgggacgtc accgacgatc ccggcggtgt
    18961 ggcgcagggc ctcgccgacc gcggtgggcc agtactccca gcccgtgagg gtgcgggtgt
    19021 cctcggggtc gtcgcggatg aacccgtcgg ggccgaacac gttgcgggtg taggactgca
    19081 ccccgaccca gtcgtcgccg gtcgactcgc gcaggtagcg gtcctcgacc aggtcagcga
    19141 acgccgccgc ggtgtcctcg ccacccggaa ccgccgagat cacctggctg gcgacggtcc
    19201 agccgacctg caggtccggt cgggcggcct tgatggcgtc gcgtgcggcg tggtgcgcgg
    19261 cgatgacgtt cacctcgctg gccaggtgcg gggcgggcag cggcgcggtc gggatgtcac
    19321 gctcccggtc ggggtcgtcg ccgccggatc cgaggtgcat gacccgggcc atgaccccga
    19381 ggatgttcgg ctcgttgatg gtggcgacgt agcggacacc gtccaggatc ccgaccgccc
    19441 gctcgacgta gccgacgaac cggtcgaccg accccgcgtc ggcccagctg ccggcgtcgg
    19501 tgacccaccg gggttcggtg aagtggtgga gggtgacgac cggttcgatc ccccgctcga
    19561 gggcaccgtc gatcatcgcc cggtagtgcg ccagcgccgc gtacgacagc tcccccgggg
    19621 ccggttcgat tcgggcccac tcgatgctga agcggtaggc gttgaagccc aactcggcca
    19681 ccaggtcgag gtcctcgccc caacggtgga agctgtcggc cgcgtcgccg ctcggctcgg
    19741 cgacgtggct cgacgggtgg tgctcgatgg cccaccagtt gctgttggtg ttggccccct
    19801 cgatctggtg cgccgcggtc gacgctccga acaggaaccc gggcgggaag cgatgctcgg
    19861 ccatcagccc tccgtcggcg gggtcagggt ggcgacctgc gggccggccg ccgcgaccag
    19921 ggcgtcgagt tgcgcggccg cctcggggtc gtccttgagg tactggtcgt agaaatcgac
    19981 cgtggcccgg gtgacgagct cgtcgagcgg ttcgtcgagg ccctcgaagt agccggggtc
    20041 gtggccgctg ccgaccaggg tcagcagtcg gcgggtgccc gggtacatgt cgaacagggc
    20101 ctggccggtg gcgtacgaca cggtcggatc ggcatcgccg tggaccagca gcgcgggcgg
    20161 cgccgactcc gccgggacgg ggccgggcga gccgaggtcg gcccacgcgc cggcgaactc
    20221 ggtgatgacg tcgacgcggt cgtccgcgca gcacgggttg atggccacgg ccacggtcgt
    20281 gatcgccccg aaggactgac cgccgaggcc gatgcggtcc gggtcgacca gggcggccca
    20341 cggtcgtccg gggtccgtcg ccgaggcgag cacctggtcg atcacgaagc tgacgtcacc
    20401 ggcctgctcg gcgatgccgc cgtagtcggt gccgccgggg gtgccctcct tggagagcgg
    20461 gaagtcgggg ccgaccacga cgtagccggc cgacgcccac gcctcgaggg tcttggcgta
    20521 gtgcaccgcc cgtcgggtgc tgccgtggga gaacacgatg agcgggaacg tcgcgtcctt
    20581 ctccccggcg gcgcccttct tcccggcggc cgccttcggg taccagacct cgagcggcat
    20641 cgtccgcgac ggcagctcgg cctgtccgcc gtgggccttg gtcggtcggc tgtcgtcgac
    20701 cagcgtcgtg gtgtcccggc cgacctcgaa ggtcccgatc tcggccaccg caccctccga
    20761 gctctccggg ctctccgact tctccgctga cgtcgtcgtc gcgtcggccg acgagtcgcc
    20821 gtcgtcgtcg gaggcgcagg ccgcgaccgc gaccagtgcc accgccgcca gggcgaccac
    20881 ggcgaggggg acgcgacggg aacggcggtc ggggcggcga gatgtgttca tggcacccgc
    20941 ggcgggagtc gaacccacgt ccggtgctcg gatacccagc gccggacgcc aaccggacag
    21001 cacgggccgg acggagcagc cgcatggtag gccgacccag gaccggtcgg gatcgggtac
    21061 cggtcacagt tcggagctcc agcagcatgg gtgtgcaagg ctggagcgat ggacgacgag
    21121 gtcccgacga ccacgttgga actggcgctg gcctggccga cgcgggtcgc ccgagaggtc
    21181 ctcctcgccc cgtggacgat ccaacgggtg cggcgctccc tcggggtcct gcccgacgag
    21241 ctcgaggcgc tgcgcaccac cctcaacgac accacccagg tgctggagga gaccctcggc
    21301 ccgctcgacg gccgactcga gaacgtcgcc ggcggcgtca accggctcga cgccgcggtg
    21361 accacgctgt cgggtgacct gatgggcctg ctcggcgagc tcgaacgcct gcttccggag
    21421 ctgtcgggca tggtcaacgg gatggacgga cgggtcgagc gggtcgagct ggtggtcagc
    21481 cagttgagcg acaccctcct ggccgttgcc ggtgcggtcc ccggcgtccg ccgcgccgtc
    21541 cggggcgccc gggccctccc cgccgacagc acccccacct gagccccgct ttgtcccgca
    21601 ccagcgcggt gatcaccgcg ctggtgcggg acaaagcgtc agggcgtggg cgggttggtc
    21661 gcggtgacca cccaggccgc accgtcgagc atcacgcccg agtcggtctc gtgcggtcgc
    21721 agcgcgtcgc gggcccgctc gatcgcctcg gcccgctgct ccggggcggc gtcaccgacc
    21781 aggatccgcc ccatgccggt gcgggtcatg aactcgaccg cggcgtcggc gtcggctccc
    21841 atccacagag ccgcggcgac cggctcgacg tcgatgtcga cgaaaccggc ggccgtcagc
    21901 atctgccggg tcaggtccgg gtcggccagc gagaacggac cgggaccacc cgagtttccg
    21961 ccgggcagtc cgagcaccga ggcgaccgcg ccgcccggca cgatcatcca ctcgttggcg
    22021 aacagctcct gccagcacgc gaacgcgatc cgaccgccgg ggcgcagcaa cgaacgcagg
    22081 tgggagaacg cgccggtcgg gtcgtcgaag aacatcaccc cgaagcggct caccagcacg
    22141 tcgaagggag cgtcgacctc gacgcgggcg gcgtcggcga cggcgaaccg ggcggcggac
    22201 agaccggcct ccgcgctgcg ccgcgacgcc accgccacct cggcgggtga caggtcgacg
    22261 ccctggacca ctccgtccgg gcccaccgcg accgccgcga gcagggacag gtccccgttg
    22321 ccgcagccca cgtcgatcac ccgctcgccg ggctgcggtg cggccgcggc caggacggcc
    22381 tcgccgtacg gggcgagcat gcgggtgtag tgctccgcgt actcggccca gtgctcgccc
    22441 tcgacggtct cccactcgct catcgcgacc tccattcagc tttgtcccgc gagagcgcgg
    22501 tgatcaccgc gctatcgcgg gacaaagcct gccgaccggt cggcacggta ccggacctca
    22561 ccggcgatga cggtcgatcg gacgccgagc gcgtccaggt cggtccgctc gtcggtgggg
    22621 tccgcgtcga gcacgaccag gtcggcgcgc cggcccgtcg ccagcgagcc gagctcgtcc
    22681 tcggtgccgc gggcgaacga tccgccggtc gtgaacaggg ccagggcctc cgagcgggtc
    22741 aacgcctgct cgggggcctg gccgccgcgg tcgaccgcgg cggccatggc ggccagcacg
    22801 tcggtccgct cgatcggtgc gtccgaggat cctgcgatcg acaccccgcc gctcgtcatc
    22861 gacgcgaagg cgtaggtccg acccaggcgg tcgagcccga cgcgacgggc gagccagtgg
    22921 ctctcgctgc gcaggttgat cggctgcacc accgcggtga tcccgtgccg ggcgaacagc
    22981 tcgatggtgg ggtcgtcgag aaccgaggcg tgctcgacgc ggtggcggtg ccggccggga
    23041 tggcggacca ggagccggtc gaacagctcc gccgcgctgc ggttggcggc gtcgccgatg
    23101 gcgtgcacgc agatctgcaa cccggcgagg tgcgcggcct ccatgcgacg gtacgcctcg
    23161 tcgtcgtcgg tggtgcgcat cccgtgctcg tgggggtgat cggcgaaggg cccgtgcatg
    23221 caggcggtgc gcccaccgag ggtgccgtcg aggaacagct tgaccgcgtc cagccgacga
    23281 ccgcgggccg ggtcgtgcag agacgggtcc cggaggtcga acacctcgga cggctcgggg
    23341 gtgatcagga tggtctggat gtcgaacggc aggtcgcccg acagcgccgc ccagcccagc
    23401 gcctccatcg ctccggcggg accggcggga ccctcgtcgg cgctctggca catggcggag
    23461 atcgacgtga cgccctggct ca
//
LOCUS       DAOQOD010000028        22967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_756_length_22967_cov_12.420784, whole genome shotgun sequence.
ACCESSION   DAOQOD010000028 DAOQOD010000000
VERSION     DAOQOD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 22967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22967
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_22967_cov_12.420784"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLZ72_04650"
     CDS             complement(<1..516)
                     /locus_tag="PLZ72_04650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA35624.1"
                     /translation="MDTDDATGRGSRAAAPDAVGPIRVDAEPLHYEPALDGIRAVAVL
                     AVMAFHFLGAEYFAGGLIGVDVFFVLSGFLITSLLLDEQNRTGAVSLRGFYHRRVLRL
                     FPAMYTLLGLVAVAAIFIGAEYPSVWAELGAAALYSYQIFLGLFGFATPDSPRVLFHL
                     WSLSVEEWFYFF"
     gene            complement(520..>1915)
                     /locus_tag="PLZ72_04655"
     CDS             complement(520..>1915)
                     /locus_tag="PLZ72_04655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA35625.1"
                     /translation="HPHDSHPHDSRLHDSGLDRTVTDTARLVRVDAPRLGHIPSFDGF
                     RGIFVLQVVLYHAGVTAKLLPGSPIIIDWFFVASGFLITSLLLDEQNRSNTIDMRRFY
                     SRRALRLFPAMYAMIAVFTVLMLAVQILAPDALENAGLWWVESLAASLYVYFLVAAFF
                     PATIGAIGHTWSLTVEEQFYFFWPMILKRSLKRGTRRSDRNLIIGCLVFIAIFVTIRM
                     SFQHVVTWDGLEAHFVDEDHLSWQGVLYRIAAVRPDMIVCGCLAAFVARAIPRPLTDQ
                     VRRIIGVLGAAGWALFVAALLFATRFPFFTMFGSVGYQIALWGLVPMILDLYFRQQSL
                     AARGLAWQPAQWLGQRSYGIYLWHIPVLLPFLAAIESSYGVRKLAIGLVAAGLGVVAG
                     LLSYRYIERRFLRIKETRFTAPQDRMRSEMAGGEIRDPGPGPLHPRHHRPAPDLPAPD
                     RPAADLPADRGADS"
     assembly_gap    1916..2015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2237..4297)
                     /locus_tag="PLZ72_04660"
     CDS             complement(2237..4297)
                     /locus_tag="PLZ72_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA35626.1"
                     /translation="MAAAKTPATNRSDTYDEKSIQLLEGLDAVRKRPGMYIGGTGGEG
                     LHHLVWELIDNAVDEAAAGHANLIEVTLHRDGSVEVADNGRGIPIGKKDGTRTALEIV
                     FTELHAGGKFGGGAYSASGGLHGVGASVVNALASKLVAEVDRDGATHRLTFVHRVPGR
                     VDAKGHFKAGSGLEVVRKVPPKRTGTRVRFWPDLDIFDPGLSIDSELVRDHCAQVCFL
                     VPGLKVRLVDKRAPGREPEEFLARGGLADLVETLSIGEPVTDVVTLHGVGTFEEKIPV
                     DGKMSLVERTCTVDVALRWVKGYDTVLSSFVNTIPTTEGGTHVSGFDRALTKVANDAL
                     LSGAKKLAKLSKSGKDRAEKSDVQEGLVAAVKVTFPEPQFKGQTKQELGTPAIGSIVY
                     EVVRDNFGNWIDGGGKKTHVNALREKLTQAVLTRVQTKANLEARRKASALSSAGMPDK
                     LADCRVHGPDAELLIVEGDSAAGPAKAGRNAEYMAVLPLRGKVVNAGKSTLKQVVENA
                     EAQALFTAIGAGSGKDFKLADARYGRIIILCDADVDGSHIRCLLLTLIHAYMRPLLED
                     GRVFAAQPPLYTVKVGDRTIHAFSEEEKLALTDELTRGNRKAENLQWVRFKGLGEMDV
                     EELAVTCLDPGTRILRRLTMDDAQAAADAAELFDTLFGNDVAKRRQYLLENSDLVDRE
                     ALDV"
     gene            4396..5655
                     /locus_tag="PLZ72_04665"
     CDS             4396..5655
                     /locus_tag="PLZ72_04665"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNA35627.1"
                     /translation="MTDSSPPPLVLDDLAAPRFGPEAAEVMALGAALANDVELAPGRL
                     VDDAIATAGGLDDFGPDGWQEPLEVICRAYRTEAGLSRFGTVSIHAQLVQLLANRLLI
                     AEVIRRHPQALEEEVRAPIVIAGLPRTGTTHLHNLLSADPSLRFLPYWESIEPVPAAG
                     EPMFGDGNLAPRTARCDAAIDMAEMWTPELKRMHEMSTWHAHEEIHLLAIDCSSMYFD
                     TLAVVPSWREYYRSQDQTPHYRYLRTVLQVLQFLRGGDRWVLKSPQHLEQFGPLSTVF
                     PDAIVVVTHRDPAEVVVSMATMIAYVARVHVEAPDPVAIGRHWSGVLGEMLGACVSDR
                     GLLADERSTDIRFEDFMADEAATVSAVYRLAGQPLDGRAAAAHAEYLAGHRRNRHGRV
                     LHDAAAVGIDRDTLSAATSDYRDAFGV"
     gene            complement(5733..6989)
                     /locus_tag="PLZ72_04670"
     CDS             complement(5733..6989)
                     /locus_tag="PLZ72_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNA35628.1"
                     /translation="MKIRSLLIGAAAVATIGLSACSGDPGTPPPADDGRYVAPTGVDT
                     GNCSASASPCATITYAQTQAVAGDTIHVAAGTYPELVIVTKPLVFEGANAGKSDGAQP
                     VTRGPESIVKGFRTPGEPHPTPDGANDVTVDGFTIDPQGDAALIAPATHHLVALFGGN
                     DVKVINNRFDGGPWVPDCDYTCTTMTDAALMIRSGTYEVKNNSFTDFRSPMDVDQDSA
                     AHPVDSATITGNSFTHVTNRAIWISEYPGGPMPGTVTVSGNEFDATGWTDDSYPAAMV
                     ITEGGNTVADNTFTGFSAAVFLQVCDGTNVAGDPNTFTGNTFTDNRTGLYYFVAGACG
                     STVVPATITGNSFDGGPWGFDPALAKIGVRWNEAGGPRPNDLTAECNWWGDVAGPNSP
                     GASQTTAGVDADPWNVTPTGDCTGTV"
     gene            complement(7091..7558)
                     /locus_tag="PLZ72_04675"
     CDS             complement(7091..7558)
                     /locus_tag="PLZ72_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA35629.1"
                     /translation="MTARPVPENYPRLSPYLAVDGATEAMRFYCDVLGFAPRGDVMTM
                     PDGRVGHAELELGGSLLMISDEYPEAGNLGPTTVGGSPVMLSIYVDDVDDVFARAIAA
                     GATAVREPEDQFYGDRMSAIVDPWGHRWSIATHIEDVDSAEMARRAAAAMGGG"
     gene            complement(7698..8834)
                     /locus_tag="PLZ72_04680"
     CDS             complement(7698..8834)
                     /locus_tag="PLZ72_04680"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HNA35630.1"
                     /translation="MTDDAIDPVPTNPAPLTAAAVGELIEALAGLDQRFLSGDRAQSD
                     LPTVLDGYRWMFSILSVGLDVFLWGDVTAPRFTDIVGANRKWGGDNADAYYQYAPIDP
                     TRTYRVRARRGDAAYFSLTVYGGPDDGRYSERIVATVNDHDVRAVDPDGTAEEFEVWL
                     SADPPPRPDPGTPPRVWLRLEPDAVAAITRDYLAEPTVGRRMEWHIDLVGDPGDWRRT
                     DESEARRYRAALTWLREQAQMVPVVLAEPNTVADPYPVPSETFGWAAGDAAYAMGNYR
                     LDDDQALVLRGRSPQCAFWNLCLWNPFLHTFDYDHDRVTINGFQVDHDPDGSWEIVIS
                     ESDPGHRNWVRTQGHRHGLIWFRWFHPTNTPDPVTAEVIHLGGA"
     gene            complement(8941..11382)
                     /locus_tag="PLZ72_04685"
     CDS             complement(8941..11382)
                     /locus_tag="PLZ72_04685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HNA35631.1"
                     /translation="MPRQTELFEGGYAREVIEVPVTKELGESFMAYSLSVITSRAIPD
                     VRDGLKPVQRRILHAMADMGMRPDRPHRKCANVVGETMGKYHPHGDGAIYDALVRMGQ
                     SFGRNITLVDPHGNFGSLDDPPAAYRYTECRLTEAAMEMLAEIDEETVEFRPTFDGER
                     SEPTYLPARLPNLLVNGTSGIAVGMATNMAPHNLVEVFEAIKLVMTKRRPKPTTAELM
                     AALPGPDFPSGGVIVDDGLAEAYETGRGSVRVRATAEVVDLTRARQGIVVTELPYLVG
                     PERVVGRIQELMRNGKLPLVTDVKNTSDRHTGLRIEIELRPGANARAVLTELYRLTPM
                     EDTFGINNVVLVDGTPRTLGLYDLCQHYIDHRLDVVRRRTEFRLEKARRRLHLVEGLL
                     IALDAIDLVVSIIRSSEDAAEARDRLMAELSLTEVQAVHILDMQLRRLTALAKLELEA
                     EAGELRGRIDDFEKILASDRRQRTIVLSELEEMVGNYGTERRSRIVSPDALDDVTLEE
                     AEAEEAANRTEEPCVVALSTTGMIGQEPVSGPRPATPGRHDVIAQRVATTTHSRLAAV
                     TSRGRVFPLSVATIPEVTGRSRGVALGELVPLDKGEEVLTLVAAGAGPDGDAPPVLLV
                     TAQGVMKRVAATDLLGVTAGRVAIKLKPQDRVVAATLAADGTDVVAVGSNAQVLRCEA
                     ASVPVQGPGAGGVAGMKLSDGAVVVGAGIAGPGAVVLTVSDSQTAKVTDAAELPTKGR
                     NTGGLRITKFRNEKRLEWAYVGTDVGVLVVVGTEDAPSKPDPTPETLTLHATGRDLAS
                     KATKRRWLGVGTGRW"
     gene            11475..13049
                     /locus_tag="PLZ72_04690"
     CDS             11475..13049
                     /locus_tag="PLZ72_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNA35632.1"
                     /translation="MAVTTDGVPSASASSDGPWLGDACSLVDAFRAGERSPAEELQAT
                     LDAIASSDLNCFSFLDPERALAAARQADLTKPFGGVPVGIKELEQVQGWPDTGASLVF
                     KDQVASFTSETTRRFIEDGGANPVGLTTASEFGGLNVSVTKLNGVCHNPWRYGRTVGG
                     SSGGSASAVAGGLVSIASGGDGGGSIRIPAGYTGLFGMKGTFGRMSRGPHAFSRPSTV
                     VNGCLARSVRDAARHFDVCAGVDPRDPTSLPDPGGWERNLGSTELKGRKVAILPSIAG
                     VPLEAGVEDEIRARAADLVEMTGMEVVDISLELPNLAAQWAMGNLSTLLAELGPRWPS
                     CAADLTDEIALGVIMAQTTYNLNLAAVAEAQRLAANEAMASAFEQVDYIVCATNPGPA
                     FAADATTSSPTSTFLDEAKASNAARLAFRGLMGGVRVANGVFPRLSNKLIEMVVERVP
                     DLVTMGGLTIISNIYGNPAVSIPAGTVDGLPVGMQVLARHHRDAELFDIGYAYEREVG
                     WSMVAPSVAARVAAPA"
     gene            complement(13080..14108)
                     /locus_tag="PLZ72_04695"
     CDS             complement(13080..14108)
                     /locus_tag="PLZ72_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA35633.1"
                     /translation="MDLGLQLGYWGQLPPPDWVERAVEAERLGFNSVWTAEAWGSDAF
                     SPLAYLAALTERVQLGTSVVQLAARTPTATAMSAITIDHMSGGRFRLGLGVSGPQVVE
                     GWYGRPSNKPLARTREYVEIIRRVVAREEPLDFQGEFHQHPYHGEGSVGLGKSLKSIV
                     HPLRRQIPIYLGAEGPKNVTQTAEIADGWLPLYYSPFRQDVYADQLTGARDGFQVAAL
                     ALFQVTDDDEPGTIEDALAGARAMLAFYIGGMGAKGQNYHTKLAARMGLGEQAERIQD
                     LFLEGRGDEAVFEVPVEFADEISLVGTPERIADRVAAWKDSAVTEILVAAGSVDQVRQ
                     AAEIILPV"
     gene            complement(14140..14946)
                     /locus_tag="PLZ72_04700"
     CDS             complement(14140..14946)
                     /locus_tag="PLZ72_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA35634.1"
                     /translation="MPAVLTEREGHLLIVTLNRPERMNAINGEMLVLMLDAFVEADQD
                     PDIRVIILTGAGGNFCSGADLKDMAGGFESNSESVVDVQARMAEDPDLHWKALLRSWR
                     PNVPIILAAEGTAIAGGTELLGATEIRVAGESAKFGISEVKWSLYPMGGSAVRIPRQI
                     PYTVACELLLTGEHITAQRALELGLIGHVVPDGQALAKAKEIGHVIGDYSAPLAVKAL
                     LRTLRETNGMEESEALAHEFTYGWDVFASEDAKEGPRAFKEKRKPNFLGK"
     gene            complement(15017..17713)
                     /locus_tag="PLZ72_04705"
     CDS             complement(15017..17713)
                     /locus_tag="PLZ72_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA35635.1"
                     /translation="MRWTRRGDDRNDERDEVIDERPVAFEQLLDRLFARFLSVATAEL
                     DTALHTTVAELGAFVGADRSYIIRYDWSAMTTTMTHEWCAEGIAGCFEMEQGLPMAVG
                     PRQQDRLEALEVNQIDDVGLLGPGWEEDIEFLQSEDITAILEVPFALDGRLAGVIGFD
                     VVTGPFTWRSSDVTALKAVASLLANVLARTLAEGALDDTVRELATVFNGAPVPLLLLD
                     RFGTVLRANQSANDMFGSRTDELAGSNALERIHPDDLAEASPQWAAMLRPDGPDRSAQ
                     EVRFLTPAGYRWHRVDAVASRTPTGEFQFSTVHLIDVDEVHRTATQLGRSERRFGSLV
                     ESLPDAVMRFDRSHHVTFANAAAVRTSQEMAASGVVMRAGWPRLDRTSASILRESLAI
                     VFKKGRPVTVEYAIGSGTTEVWCESTFVPEFAADGSVESVLLVARDITHRRDHEAELA
                     HRATHDALTGLPNRSMLLSLLSAAVVERADRHDDGRCLALLFLDVDRFKTVNDSLGHG
                     TGDELLCRVAERLGTTLRPHDTLARLGGDEFTVLLPDIALGEATTVAARLQDSLREPI
                     DINGTTFRPTVSIGVVETSDTAEPTDLLRWADAAMYEAKAGGRNRVAIFDERMRAEVS
                     ERNELDRDLTGAVDRNEFVLHFQPEVELATGRPLGCEALVRWRHPDRGTMSPDRFISL
                     AEENGTIVALGRWVLLDACRTVVGWRTAGLVADDFILRVNLSARQLDQAGLARDVADV
                     LSTVGLPPSRLCLEVTETALMRDVRAGMAALTELHRVGVQLAIDDFGTGYSSLSYLKR
                     FPIDVLKVDRSFVDGLPGEAHDVAITTAILDLARSLELEVTAEGVETVGQRDALIELG
                     CKRAQGYLFARPMPAEDLVRHLMQQQASATSAAEPVAAANPG"
     gene            complement(17802..18608)
                     /locus_tag="PLZ72_04710"
     CDS             complement(17802..18608)
                     /locus_tag="PLZ72_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA35636.1"
                     /translation="MTVLDRFRVDGQVALVTGAGRGIGRACALALAEAGADVVVAART
                     AEQVDAVAEEARALGARAESVTFDVMDLDRLDELVAVATERLGGLDIVINNAGGSFPR
                     PLLETSVASFERAFRFNVTTALELTKSAVPPMLARGGGSVVNISSAIGRMSDRGFAAY
                     GTAKAALSHLTRLMAADLAPHIRVNGIATGSIATSALDTVLTDDALRTAMEEATPLRR
                     LGRPDDIAAGVLYLCSPAGSYLTGKLIEIDGGLQAPTLGLGLPDLEPGPT"
     gene            complement(18605..19951)
                     /locus_tag="PLZ72_04715"
     CDS             complement(18605..19951)
                     /locus_tag="PLZ72_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNA35637.1"
                     /translation="MNVDLLEKSARVIAGDAPPGEFGSIGLNEAPELHVLDDDIAFVA
                     AFSNVAAFTAPAGAGRSVVEDELLLIDVSSAFHGRTVHELVRSWTDAPLSTAVYTHGH
                     TDHVGGITWFEQEAAERGRLAPEVVAHERVVDRFDRYRMTAGYNGVINQRQFRLPEPW
                     FPTEYRYPDRTYRGSATATVGARTVELHHSRGETDDHTWAWIADRKVLCAGDMFIWVS
                     PNCGNPQKVQRYPMEWAAGLRRMADLGAELLMPGHGPPIAGSDDIARVLGSAAEYLES
                     ITSQTLEMMNAGARLDDIVHTVRVPDELAGLPWLRPIYDEPEFVVHNIWRLYGGWYDG
                     NPARLKPAPDAAVAAELAALAGGASVLAERARALAAEGDLRLAGHLAELAAQAAPGDS
                     GVHAVRSEVFGARARAEASLMASGVFRWAARESADASGMTGDEPGSTDPGAGTADR"
     gene            complement(20080..21105)
                     /locus_tag="PLZ72_04720"
     CDS             complement(20080..21105)
                     /locus_tag="PLZ72_04720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35638.1"
                     /translation="MDLLPTPEQEEIVTTVRAQLERRFALNDLAANDGSVQVVDRDLW
                     RQCAELGWFGLGLDEELGGVGYTLVEESLLFAELGAHATPGPFLATVLGARLAATAGE
                     GELAAAILAGDVLVALAEAEDARASVGDEVSGRFRVTDHGGADVLLVAGSDPAAGLAL
                     VDAVAASVEPSIDLLTPLGVVDLAGVTARASVADSSELALRATILIASQLAGIATATA
                     AQSVEYAKDREQFGRPIGSFQAVKHRCADMAARAEAATSLSRYAALAVVDGRTDGAFH
                     AQAAWTVASEAAVTNAQVNIQNHGGIGFTWEHTAHRFLTRAQFLVRTLGDRRHHLSAL
                     LAEPAPT"
     gene            complement(21118..22296)
                     /locus_tag="PLZ72_04725"
     CDS             complement(21118..22296)
                     /locus_tag="PLZ72_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35639.1"
                     /translation="MNLDLTEEELAFREEARTWLRENLPTEERPPEGQEMRAFDLAWQ
                     RTLYDGGWAGISWPVDFGGRGLSDIQQMIWYEEFARANPPFTLNNSCTFVGNNHGGPT
                     IIVNGTDEQKATYLPPILRGEVVWCQGFSEPGAGSDLGSLTCRAEIDGDHLVVNGQKT
                     WTSYAHVADLQELLVRTDPDAPKHKGISWVVCDMTTPGIEIREIRTMSHVTDFCEVFY
                     DEVRIPLTNVVGGLHNGWRTAMSTLSFERGTAFMADQVELTSTIERLIGEARERTDAH
                     GRPLIGDEEVARRLATARAEVDALKAMTIAGISRTARTGMPGAEGSMIRFFHGELHQR
                     VYQLALDIIGADALRLTPVDGDVWTGPYLQSFAYTIGGGTTDIQRNIVGERVLGLPRA
                     "
     gene            complement(22370..>22967)
                     /locus_tag="PLZ72_04730"
     CDS             complement(22370..>22967)
                     /locus_tag="PLZ72_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35640.1"
                     /translation="PTPPPPIEAPTQVTNPTVPVPPVAPMTPAPPAPPGAPGAAPGYA
                     PMGPGGTPPYAAPGAPGYGAPPVGPPGVAPGYAAPVGAEAPKKKSKAGLVIALLVVVL
                     LLVGGAVAFLIAGSGTGPTATIRECRIDADGSLTAAGKVDGADESAKVHVEFRNSDGN
                     DVVDSGSTDASSSGTWEVTGSASDDVQKVTCVVTRVGS"
BASE COUNT         3172 a         7816 c         8420 g         3459 t
ORIGIN      
        1 gaagaagtag aaccactcct cgaccgacag ggaccacagg tggaacagca cccgggggga
       61 gtccggcgtg gcgaatccga acaggcccag gaagatctgg tacgagtaca gggccgcggc
      121 cccgagctcg gcccacaccg acgggtactc ggccccgatg aagatcgccg cgaccgccac
      181 cagccccagc agcgtgtaca tggccgggaa gaggcgcagg acccgtcggt ggtagaagcc
      241 gcggaggctg accgcaccgg tgcggttctg ctcgtcgagc agcaggctgg tgatgaggaa
      301 ccctgacagc acgaagaaca cgtcgacgcc gatgagccca ccggcgaagt actcggctcc
      361 gaggaagtgg aacgccatca ccgcgagcac ggcgaccgcc cggatgccgt ccagcgccgg
      421 ctcgtagtgc agcggttcgg cgtcgacccg gatgggtccg acggcgtcgg gggcggcggc
      481 gcgggaccca cgcccggtgg cgtcgtcggt gtccatcgat cagctgtcgg cgccgcggtc
      541 ggcaggcagg tcagcggcag gccgatccgg ggcgggcagg tccggggcgg gtcggtggtg
      601 ccgggggtgg agcggtccgg gtcccgggtc gcggatctcg ccgccggcca tctcggagcg
      661 catccggtcc tgcggagcgg tgaagcgggt ctccttgatg cgcaggaacc ggcgttcgat
      721 gtagcggtag ctgagcaggc cggcgacgac gcccagcccg gctgccacca gaccgatggc
      781 gagcttgcgc acgccgtagc tcgactcgat cgcagccagg aacggcagca gcaccgggat
      841 gtgccacagg tagatgccgt aggagcgctg ccccaaccac tgggcgggct gccaggcgag
      901 accccgggcg gccagggact gctgacggaa gtacaggtcg aggatcatgg ggaccagacc
      961 ccacagggcg atctggtacc cgacgctgcc gaacatggtg aagaacggga accgggtggc
     1021 gaacagcagg gcggcgacga acagcgccca gccggcggca ccgaggaccc cgatgatgcg
     1081 ccggacctga tcggtcaggg gtcgggggat cgcccgggcg acgaacgcgg ccaggcaacc
     1141 gcacacgatc atgtcggggc gcaccgcggc gatccggtag agcaccccct gccagctcag
     1201 atggtcctcg tcgacgaagt gggcctcgag cccgtcccag gtgacgacgt gctggaagct
     1261 catccggatc gtgacgaaga tcgcgatgaa gaccaggcag ccgatgatca ggttgcggtc
     1321 cgaacgccgg gtcccccgct tgagggaccg cttcaggatc atgggccaga agaagtagaa
     1381 ctgctcttcg accgtgaggc tccacgtgtg gccgatcgcc ccgatggtgg cggggaagaa
     1441 ggcggcgacc aggaagtaca cgtagaggct ggcggcgagg gactcgaccc accacaaccc
     1501 ggcgttctcg agcgcgtcgg gcgccaggat ctgcaccgcc agcatcagga cggtgaacac
     1561 ggcgatcatg gcgtacatgg ccgggaacag ccgcagggcg cggcggctgt agaagcggcg
     1621 catgtcgatg gtgttgctgc ggttctgctc gtcgagcagc agcgacgtga tcaggaagcc
     1681 gctggccacg aagaaccagt cgatgatgat cggcgacccg ggcagcagct tggcggtgac
     1741 cccggcgtgg tacagcacga cctggagcac gaagatcccg cggaagccgt cgaaggacgg
     1801 gatgtgaccc agacgggggg cgtcgacccg gaccaggcgg gcggtgtcgg tcacggtgcg
     1861 gtccagtcca ctgtcgtgca ggcggctgtc gtgcgggtgg ctgtcgtgcg ggtggnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctg tcgtgcgggt ggctgtcgtg
     2041 cgggtggtcg tcgcatcggg cgcgaccgtg gggccgtcct cggcctgctc cctcatggat
     2101 gcgccccgca tgtgccccgg tacccggtcc ccgatgtcga ccacccccga gccctcgacc
     2161 cggaacccgt caacggtacc gacgtcgacc ggggctgtcc acggacgcgg acggacggcg
     2221 accgggggct gcggtctcag acgtcgagcg cttcgcggtc gaccaggtcg gagttctcga
     2281 gcaggtactg gcgtcgcttg gcgacgtcgt tgccgaagag ggtgtcgaac agctcggcgg
     2341 cgtcggccgc ggcctgggcg tcgtccatgg tgagtcggcg caggatgcgg gtgccgggat
     2401 ccaggcaggt gacggcgagc tcttcgacgt ccatctcgcc caggcccttg aaccgcaccc
     2461 actggaggtt ctcggccttg cggttgcccc gggtcagctc gtcggtgagg gcgagcttct
     2521 cctcttcgga gaaggcgtgg atggtccggt cgccgacctt gacggtgtac agcgggggct
     2581 gggcggcgaa cacccggccg tcctcgagca gcggccgcat gtaggcgtgg atcagggtga
     2641 gcaggaggca gcggatgtgg ctgccgtcca catcggcgtc gcacaggatg atgatgcggc
     2701 cgtagcgggc gtccgcgagc ttgaagtcct tgcccgaacc cgcgccgatg gcggtgaaca
     2761 acgcctgggc ctcggcgttc tcgaccacct gcttgagggt ggacttgccg gcgttgacca
     2821 ccttgccgcg cagcggcagc accgccatgt actcggcgtt gcgacccgcc ttggccggac
     2881 ccgcggccga gtcgccctcg acgatcagca gctcggcgtc ggggccgtgg acccggcagt
     2941 cggccagctt gtcgggcatg ccggccgagc tgagcgcgga cgccttgcgg cgcgcctcga
     3001 ggttggcctt ggtctgcacc cgggtgagca ccgcctgggt caacttctcc cgcagggcgt
     3061 tgacgtgggt cttcttgccg ccgccgtcga tccagttgcc gaagttgtcg cgaacgacct
     3121 cgtagacgat ggagccgatg gcgggggtgc ccagctcctg cttggtctgg cccttgaact
     3181 gcggttcggg gaacgtcacc ttgacagcgg cgaccaggcc ctcctggacg tcggacttct
     3241 cggcgcggtc ctttcctgac ttggagagct tggccagctt cttggccccc gagagcaggg
     3301 cgtcgttggc gaccttggtc agggcccggt cgaagcccga cacgtgggtg ccgccctcgg
     3361 tggtggggat cgtgttgacg aagctcgaca gcacggtgtc gtagcccttg acccagcgca
     3421 gggcgacgtc gacggtgcag gtccgttcga ccaggctcat cttgccgtcg acggggatct
     3481 tctcctcgaa ggtgccgacg ccgtgcaggg tgacgacatc ggtgaccggc tcgccgatgc
     3541 tcagcgtctc gacaaggtcg gccaacccgc cccgggccag gaactcctcg ggttcgcgtc
     3601 ccggggcgcg cttgtcgacg aggcggacct tgagccccgg caccaggaag cagacctggg
     3661 cgcagtggtc ccggacgagc tcggaatcga tggagagccc cgggtcgaag atgtcgaggt
     3721 cgggccagaa ccggacccgt gttccggtgc gcttgggtgg gaccttgcgg acgacctcga
     3781 gtccggagcc cgccttgaag tggcccttgg cgtcgacgcg gccggggacc cggtgcacga
     3841 aggtgagccg gtgggtggcg ccgtcgcggt cgacctcggc caccagcttg gacgccaggg
     3901 cgttgaccac cgacgcgccg acgccgtgga ggcccccgga ggccgagtag gcgcctccgc
     3961 cgaacttccc gccggcgtgc agctcggtga agacgatctc gagtgcggtg cgcgtgccgt
     4021 ccttcttgcc gatggggatg ccgcgaccgt tgtcggccac ctcgaccgac ccgtcgcggt
     4081 ggagggtgac ctcgatcagg ttggcgtgac cggcggcggc ctcgtcgacc gcgttgtcga
     4141 tcagctccca caccaggtgg tgcagcccct cgcctccggt gcctccgatg tacatgcccg
     4201 ggcgcttgcg caccgcgtcg aggccttcga gcagctggat gctcttctcg tcgtaggtgt
     4261 cggatcggtt ggtggcgggg gtcttcgcgg cagccatggc ggttccgagt ctcccacggt
     4321 gcgctgacat cgccgctcat ccgtggtcgt cgtccccctc gcccggcgcg tccgggaagt
     4381 gactaccgtc accgcatgac cgactcctcg cccccgccgc tggtgctcga cgacctggcc
     4441 gcaccccgct tcgggcccga ggcggcggag gtcatggccc tcggagcggc gctggccaac
     4501 gacgtcgagc tggcgcccgg tcgcctcgtc gacgacgcga tcgccacggc gggcgggctg
     4561 gacgacttcg ggcccgacgg ctggcaggaa ccgctcgagg tgatctgccg ggcctaccgg
     4621 accgaggccg gcctgtcgcg gttcggcacc gtcagcatcc acgcccagct ggtgcagctg
     4681 ctcgccaacc ggctgctgat cgccgaggtg atccgccgcc acccgcaggc gctcgaggag
     4741 gaggtccggg cgccgatcgt gatcgccggg cttccccgca ccgggaccac gcatctccac
     4801 aacttgctgt cggcggaccc gtcgctgcgg ttcctgccct actgggagtc gatcgaaccc
     4861 gtgcccgccg ccggcgagcc gatgttcggc gacggcaacc tggccccgcg gaccgcgcgg
     4921 tgcgacgccg cgatcgacat ggccgagatg tggacccccg agctgaagcg gatgcacgag
     4981 atgtccacgt ggcacgccca cgaggagatc cacctgctcg ccatcgactg ctcgtcgatg
     5041 tacttcgaca cgctcgccgt ggtcccgtcg tggcgcgagt actaccggtc ccaggaccag
     5101 accccgcact accgctacct gcggacggtg ctgcaggtgc tgcagttcct gcgcggcggc
     5161 gaccgatggg tgctgaagtc gccccagcac ctcgagcagt tcggcccgct cagcacggtg
     5221 ttccccgacg cgatcgtcgt cgtcacccac cgcgatcccg ccgaggtggt ggtgagcatg
     5281 gcgacgatga tcgcctacgt cgcccgcgtg cacgtcgagg cgccggaccc ggtggcgatc
     5341 gggaggcact ggagtggggt gctcggcgag atgctcggcg cgtgcgtctc ggatcgcggg
     5401 ctgttggccg atgagcggag caccgacatc cgcttcgagg acttcatggc cgacgaggcg
     5461 gccacggtgt cagcggtgta ccggctcgcg ggacagccgc tcgacggtcg ggccgccgcg
     5521 gcgcacgccg agtacctggc cggtcaccgc cgcaaccggc acggacgggt gctccacgac
     5581 gcggcggcgg tcgggatcga ccgcgacacg ctgtcggcgg cgacctcgga ctaccgggac
     5641 gccttcgggg tctgatccgg agccgtcacc ggctgcagca cccgatcgtc ggaacggtcg
     5701 gtgctgcagc cggatcgggt tggttcagct cctcagacgg tcccggtgca gtcgccggtg
     5761 ggggtgacgt tccacgggtc ggcgtcgacg cccgcggtgg tctggctggc gccggggctg
     5821 ttcggtcccg ccacgtcgcc ccaccagttg cactccgcgg tcaggtcgtt cgggcggggt
     5881 cccccggcct cgttccagcg gaccccgatc ttggcgagcg cggggtcgaa gccccaaggt
     5941 ccgccgtcga agctgttgcc ggtgatcgtg gccggaacga cggtggatcc gcacgcgccg
     6001 gcgacgaagt agtacaggcc ggtgcggttg tcggtgaacg tgttgccggt gaacgtgttg
     6061 gggtcaccgg cgacgttcgt gccgtcgcag acctgcagga acaccgccgc gctgaacccg
     6121 gtgaaggtgt tgtcggcgac ggtgttgccg ccctcggtga tcaccatcgc ggccgggtag
     6181 ctgtcgtcgg tccagccggt ggcgtcgaac tcgttgcccg acacggtgac ggtcccgggc
     6241 atcggcccgc cggggtactc cgagatccag atcgcccggt tggtcacgtg ggtgaacgag
     6301 ttgcccgtga tcgtggccga gtccaccggg tgcgcggcgc tgtcctggtc gacgtccatc
     6361 gggctgcgga agtcggtgaa cgagttgttc ttcacctcgt aggtgcccga gcggatcatc
     6421 agcgctgcgt cggtcatggt cgtgcacgtg tagtcgcagt ccggcaccca cggtccgccg
     6481 tcgaagcggt tgttgatcac cttcacgtcg ttcccgccga acagcgcgac caggtggtgg
     6541 gtggccggcg cgatcagcgc cgcgtcgccc tgggggtcga tggtgaagcc gtcgacggtg
     6601 acgtcgttcg ctccgtcggg ggtcgggtgc ggctcaccgg gggtgcggaa gcccttgacg
     6661 atgctctccg ggccgcgggt gaccggttgg gcgccgtcgc tcttgccggc gttggcgccc
     6721 tcgaacacca gcggcttggt cacgatgacc agttcggggt acgtgcccgc cgcgacgtgg
     6781 atcgtgtcgc cggccaccgc ctgggtctgc gcgtaggtga tggtggcgca cggtgacgcg
     6841 ctggccgagc agttgccggt gtcgaccccg gtcggggcga cgtagcggcc gtcgtccgcc
     6901 ggtggaggtg ttcccggatc gcccgaacac gcgctgaggc cgatcgtcgc gaccgcggcc
     6961 gcgccgatca ggagtgatct gatcttcatg gttccccctc tgccgtcggc cccacgccga
     7021 ctcgggggaa ttgttaccag acccaggggc cggggtgccg ggaagctgac ccgtgggacc
     7081 cgggaccggg tcagccccca cccatcgcgg cggcagcccg tcgggccatc tccgccgagt
     7141 cgacgtcctc gatgtgggtg gcgatgctcc agcggtgacc ccacggatcg acgatcgccg
     7201 acatgcggtc cccgtagaac tggtcctcgg gctcgcgcac cgcggtcgcg cccgccgcga
     7261 tcgcccgggc gaacacgtcg tcgacgtcgt ccacgtagat gctcagcatc accggcgagc
     7321 cgccgacggt cgtcgggccg aggttgccgg cctcggggta ctcgtcggag atcatcagca
     7381 gcgagccgcc gagctcgagc tcggcgtgtc cgacgcgacc gtcgggcatg gtcatcacgt
     7441 cgccccgcgg ggcgaagccg agcacgtcgc agtagaagcg catggcctcg gtcgcgccgt
     7501 cgacggccag gtacggcgac agacgcgggt agttctcggg aacgggtcga gcggtcatgg
     7561 tggcgcctcc tgggtggtgg ctcccagtca acactccggc cggggtcgac gggggcgggc
     7621 ccgagggatc tggccgaccg gacaggacca ccgcgacggt ggggccgcgt ctccgggccg
     7681 ctccgtcgga gcgatgctca cgccccgccg aggtggatca cctcggcggt caccggatcg
     7741 ggggtgttgg tcggatggaa ccagcggaac cagatcaggc cgtggcggtg cccctgcgtg
     7801 cggacccagt tgcggtggcc ggggtcggac tcgctgatca cgatctccca ggacccgtcg
     7861 ggatcgtggt ccacctggaa cccgttgatg gtcacgcgat cgtggtcgta gtcgaaggtg
     7921 tgcaggaacg ggttccacag gcacaggttc cagaacgcgc actgcggtga ccggccccgc
     7981 agcaccaggg cctggtcgtc gtccaaccgg tagttgccca tcgcgtaggc ggcgtcgccg
     8041 gcggcccacc cgaaggtctc cgacgggacg gggtacgggt ccgcgaccgt gttcggctcg
     8101 gccagcacca ccggcaccat ctgggcctgc tcgcgcagcc atgtgagcgc agcgcggtag
     8161 cggcgggcct cggactcgtc ggtgcgacgc cagtcgccgg gatcgcccac caggtcgatg
     8221 tgccactcca tgcgtcggcc gacggtcggc tccgccaggt agtcgcgggt gatggcggcc
     8281 acggcgtcgg gttccagtcg gagccacacc cggggcgggg tgccggggtc gggtcgtggc
     8341 ggcgggtccg ccgacagcca cacctcgaac tcctcggcgg tgccgtcggg atcgacggcg
     8401 cgcacgtcgt ggtcgttcac ggtggcgacg atgcgctcgg agtagcgacc gtcgtcgggg
     8461 ccgccgtaca cggtgagcga gaagtacgcc gcgtcgcccc gacgcgcccg cacccggtag
     8521 gtccgggtcg gatcgatcgg cgcgtactgg tagtaggcgt ccgcgttgtc gccgccccac
     8581 ttgcggttgg ctccgacgat gtcggtgaag cggggggcgg tgacgtcgcc ccacaggaag
     8641 acgtcgaggc cgaccgacag gatggagaac atccagcggt atccgtcgag aaccgtcggc
     8701 aggtcgctct gggcgcgatc gccggacagg aaccgctggt ccagtccggc gagcgcctcg
     8761 atcagctcgc cgacggccgc ggcggtgagc ggggcgggat tggtgggcac cgggtcgatc
     8821 gcgtcgtcgg tcaccgccga agttgagcag aggtgggccc cttcgtgagc tgcatcctgg
     8881 ctgcagttcg tgccggagcc tcctgaaccg cagccagaga cggctggggc ggcgcctggg
     8941 ctaccagcgg ccggtgccga cccccagcca acggcgcttg gtcgccttgg acgccaggtc
     9001 ccgcccggtg gcgtgcaggg tcagggtctc gggggtcggg tccggtttgg agggggcgtc
     9061 ctcggtgccg acgacgacga gcaccccgac gtcggtgccg acgtaggccc actccaggcg
     9121 cttctcgttg cggaacttcg tgatgcgcag gccgccggtg ttgcggccct tggtcggcag
     9181 ctcggcggcg tcggtgacct tggcggtctg ggagtcggac acggtgagga ccaccgcacc
     9241 cggaccggcg atgcccgccc cgacgacgac cgcgccgtcg gacagcttca tcccggcgac
     9301 gccaccggct cccggcccct gaacggggac ggaggcggcc tcgcagcgca ggacctgtgc
     9361 gttggaaccc acggccacga cgtcggtgcc gtccgccgcc agggtggcgg ccaccacccg
     9421 gtcctggggc ttgagcttga tcgcgacccg gccggcggtg acgccgagga ggtcggtggc
     9481 ggcgacccgc ttcatcacgc cctgggcggt gacgagcagg accggcggag cgtcgccgtc
     9541 ggggccggcg ccggcggcga ccagggtcag cacctcctct cccttgtcca gggggaccag
     9601 ctcgccgagg gccacgccgc gggagcggcc ggtcacctcg gggatggtcg cgaccgacag
     9661 cgggaacacc cgtccccggg aggtgaccgc ggccaggcgg gagtgcgtgg tggtcgccac
     9721 ccgttgagcg atgacgtcgt gtcgtcccgg tgtggcgggt cgggggccgc tgaccggctc
     9781 ctggccgatc atgccggtgg tgctgagcgc gacgacgcac ggttcctcgg ttcggttcgc
     9841 cgcctcctcg gcctcggcct cttccagggt gacgtcgtcg agggcatcgg gtgacacgat
     9901 gcgactgcga cgctcggtgc cgtagttgcc gaccatctcc tcgagctcgc tcaacacgat
     9961 ggtccgctgc cggcggtccg aggcgaggat cttctcgaag tcgtcgatgc ggccccgcag
    10021 ctcgccggcc tcggcctcga gctccagctt ggcgagcgcg gtcagacgtc gcagctgcat
    10081 gtcgaggatg tgcaccgcct gcacctcggt cagcgacagc tccgccatca gccggtcccg
    10141 ggcctcggct gcgtcctcgg agctgcggat gatcgacacg accaggtcga tggcgtcgag
    10201 cgcgatgagc aggccctcga ccaggtgcag tcgccggcgg gccttctcga gtcggaactc
    10261 ggtgcggcgg cgcacgacgt cgagacggtg gtcgatgtag tgctggcaca ggtcgtacag
    10321 gccgagtgtc cgcggtgtgc catcgaccag cacgacgttg ttgatgccga acgtgtcttc
    10381 catgggagtc agccggtaca gctcggtcag cacggcccgg gcgttggctc ccggacgcag
    10441 ctcgatctcg atgcgcagcc cggtgtggcg atcggacgtg ttcttgacgt cggtcaccag
    10501 gggcagcttg ccgttgcgca tcagctcctg gatgcgcccc accacccgtt cggggccgac
    10561 caggtagggg agctcggtga cgacgatgcc ctggcgtgcc cgggtcaggt cgacgacctc
    10621 ggcggtcgcc cggacccgga cgctgccccg gccggtctcg taggcctcgg ccagaccgtc
    10681 gtcgacgatg acgcctccgg acgggaagtc cggcccgggc agcgcggcca tcagctcggc
    10741 ggtggtcggc ttgggccgcc gcttggtcat caccagcttg atggcctcga acacctcgac
    10801 caggttgtgc ggcgccatgt tggtcgccat ccccaccgcg atgccggagg tgccgttgac
    10861 caggaggttg gggagccggg ccggcaggta ggtcggctcg gagcgctcgc cgtcgaaggt
    10921 cgggcggaac tcgacggtct cctcgtcgat ctcggccagc atctccatgg ccgcctcggt
    10981 caggcggcac tcggtgtagc ggtaggcggc cggcgggtcg tccagcgacc cgaagttgcc
    11041 gtgcgggtcg accagggtga tgttgcgccc gaaggactga cccatgcgca ccagcgcgtc
    11101 gtagatcgcg ccgtcgccgt gggggtggta cttgcccatc gtctcgccga cgacgttggc
    11161 gcacttgcga tggggtcggt caggacgcat ccccatgtcg gccatggcgt gcaggatgcg
    11221 gcgctgcacc ggcttgagcc cgtcgcgcac gtcggggatg gcccgcgacg tgatgaccga
    11281 cagcgagtac gccatgaacg actcgcccag ctccttcgtg acggggacct cgatgacctc
    11341 gcgggcgtac ccaccttcga acagttcggt ctgacgtggc atggggacca ttcaagccgc
    11401 ggtcggtgac ggtgacgggc gaccaccccg acccggggtg aatccggttc ggtccctgtg
    11461 ggaccatgga gcccatggct gtgacgaccg atggtgtccc ctccgcttcc gcgtcctcgg
    11521 acggcccctg gctgggtgac gcatgctccc tggtcgacgc gttccgggcc ggggagcgct
    11581 cgccggccga ggagctccag gcgacgctcg acgcgatcgc gtccagcgac ctgaactgct
    11641 tctcgttcct cgaccccgag cgggccctgg ccgcggcgcg gcaggcggac ctgaccaagc
    11701 cgttcggtgg cgtgcccgtc ggcatcaagg agctcgagca ggtgcagggg tggcccgaca
    11761 cgggcgcgtc gctcgtgttc aaggaccagg tcgcgtcctt cacctccgag acgacccgtc
    11821 gcttcatcga ggacggcggc gccaaccccg tcgggttgac caccgcgtcg gagttcggcg
    11881 gactgaacgt cagcgtcacc aagctcaacg gcgtgtgcca caacccgtgg cggtacggcc
    11941 gcaccgtcgg cgggtcctcc ggcgggtccg cctccgcggt cgccggcggg ctggtgtcga
    12001 tcgccagcgg cggtgacggc ggaggctcga tccggatccc cgccggctac accggcctgt
    12061 tcggcatgaa ggggacattc ggtcgcatgt cgcggggtcc gcacgcgttc tcgcgtccga
    12121 gcacggtcgt caacggctgc ctggcccgct cggtgcgcga cgcggcgcgc cacttcgacg
    12181 tctgcgccgg cgtcgacccc cgcgacccga ccagcctccc cgaccccggc ggctgggagc
    12241 gcaacctcgg ctcgaccgag ctgaagggcc gcaaggtggc catcctgccg tcgatcgccg
    12301 gagtccccct cgaggccggc gtcgaggacg agatccgcgc ccgagccgcg gatctggtcg
    12361 agatgaccgg catggaggtc gtcgacatca gcctggagct gccgaacctg gccgcgcagt
    12421 gggcgatggg gaacctgtcg acgctgctcg ccgagctcgg cccgcggtgg ccgtcgtgcg
    12481 cggcggacct gaccgacgag atcgcactgg gcgtgatcat ggcccagacg acctacaacc
    12541 tgaacctcgc cgcggtcgcc gaggcgcagc gcctggccgc caacgaggcg atggcctccg
    12601 cgttcgagca ggtcgactac atcgtctgcg ccaccaaccc cgggcccgcg ttcgccgcgg
    12661 atgcgaccac gtcgagcccg acgtcgacct tcctcgacga ggccaaggcc tccaacgccg
    12721 cccgcctcgc gttccgaggg ctgatgggcg gcgtccgggt cgccaacggc gtgttccccc
    12781 ggctgtccaa caagctgatc gagatggtcg tcgagcgggt gccggacctg gtcaccatgg
    12841 gcgggctcac catcatctcc aacatctacg gcaacccggc ggtgtcgatc ccggccggca
    12901 ccgtcgacgg tctgccggtc ggcatgcagg tgctggcccg ccaccaccgc gacgccgagc
    12961 tgttcgacat cggctacgcg tacgagcgcg aggtgggttg gtcgatggtc gccccgtcgg
    13021 tcgcggcccg ggtcgccgcc ccggcctgag gccgcccccg ccctggccgc gctgacgagt
    13081 cagacgggga ggatgatctc ggcggcctga cggacctggt ccaccgaccc ggccgcgacc
    13141 aggatctcgg tcaccgcgga gtccttccat gccgcgaccc gatcggcgat gcgctccggg
    13201 gtgccgacaa ggctgatctc gtcggcgaac tcgaccggca cctcgaagac cgcctcgtcg
    13261 ccccgtccct ccaggaacag gtcctggatg cgctccgcct gctcgccgag acccatccgg
    13321 gccgccagct tggtgtggta gttctggccc ttggcgccca tgccgccgat gtagaacgcc
    13381 agcatcgccc gggctccggc cagggcgtcc tcgatcgtgc cgggctcgtc gtcgtcggtg
    13441 acctggaaca atgccagcgc cgcgacctgg aacccgtccc gggcgccggt gagctggtcg
    13501 gcgtacacgt cctggcggaa cggcgagtag tacagcggca accagccgtc cgcgatctcc
    13561 gcggtctgcg tcacgttctt ggggccctcg gcgcccaggt agatcgggat ctgccgtcgc
    13621 agcgggtgca cgatcgactt gagcgacttg cccagaccga ccgacccctc gccgtggtac
    13681 gggtgctggt ggaactcgcc ctggaagtcg agcggctcct cgcgggcgac cacccgtcgg
    13741 atgatctcca cgtactcccg ggtgcgcgcc agcggcttgt tcgacgggcg gccgtaccag
    13801 ccctcgacca cctgggggcc cgacacgccg agcccgaggc ggaaccgacc gccggacatg
    13861 tggtcgatcg tgatcgccga catcgccgtc gcggtcggcg tgcgcgccgc cagctgcacg
    13921 acggaggtgc ccagctgcac ccgctcggtc aacgccgcca ggtaggcgag agggctgaag
    13981 gcgtcggacc cccatgcctc ggcggtccac accgagttga agccgagtcg ctcggcctcg
    14041 acggcgcgct ccacccagtc cggtggcggg agctgtcccc agtagccgag ttgcagtccg
    14101 agatccatca gttccccgat ccgtgcggtg gtgccgcggt cacttgccca ggaagttggg
    14161 cttgcgcttc tccttgaacg cccgggggcc ctccttggcg tcctcggacg cgaatacgtc
    14221 ccagccgtag gtgaactcgt gcgcgagtgc ctcggactcc tccatgccgt tggtctcgcg
    14281 cagggtgcgg agcagggcct tgaccgcgag cggcgcgctg tagtcgccga tcacgtggcc
    14341 gatctccttg gcctttgcca gcgcctggcc gtcggggacg acgtggccga tgagtccgag
    14401 ctcgagggcc cgctgcgcgg tgatgtgctc gccggtgagc agcagctcgc aggccacggt
    14461 gtaggggatc tggcgcggga tgcgcaccgc ggatccgccc atcgggtaca gcgaccactt
    14521 cacctcggag atgccgaact tggccgactc gccggccacg cggatctcgg tggcgccgag
    14581 cagctccgtg ccgccggcga tggcggtccc ctcggcggcc aggatgatgg ggacgttcgg
    14641 ccgccagctg cgcagcagcg ccttccagtg caggtcgggg tcctcggcca tgcgggcctg
    14701 gacgtcgacg acggactcgg agttgctctc gaagccgccg gccatgtcct tcaggtcggc
    14761 gccggagcag aagttccccc cggcgccggt gaggatgatc acccggatgt cggggtcctg
    14821 gtcggcttcg acgaaggcgt cgagcatcag gacgagcatc tcgccgttga tggcgttcat
    14881 ccgctccgga cggttgagcg tcacgatgag caggtgcccc tcgcgctcgg tcaggactgc
    14941 cggcatcggc ccccctcggt cggtggccgg cgcggtgccg gcgcgcgggc accctaccga
    15001 gtgacgagaa ccggttctag cccgggttgg ccgcggcgac gggttccgcc gccgaagtcg
    15061 cggatgcctg ctgctgcatc aggtgtcgca cgaggtcctc cgccggcatc ggccgggcga
    15121 acaggtagcc ctgcgcccgc ttgcagccca gctcgatcag ggcgtcgcgc tgcccgacgg
    15181 tctcgacgcc ctcggcggtg acctcgagct cgagcgaccg ggccaggtcg aggatcgcgg
    15241 tcgtgatcgc cacgtcgtgg gcctcgccgg gcaggccgtc gacgaagctg cggtcgacct
    15301 tcaggacgtc gatcgggaac cgcttgagat agctgagcga gctgtagccg gttccgaagt
    15361 cgtcgatcgc cagctgaacg ccgacccggt ggagctcggt cagcgcggcc atgccggcgc
    15421 ggacgtcgcg catcagcgcc gtctcggtga cctcgaggca caggcgcgac ggtggaaggc
    15481 cgaccgtcga caggacgtcg gcgacgtccc gcgccaaccc ggcctggtcg agctggcggg
    15541 ccgacaggtt gacccgcagg atgaagtcgt cggcgaccaa ccccgcagtg cgccagccga
    15601 ccacggtccg acacgcgtcg agcagcaccc atcgtccgag ggcgacgatc gtcccgttct
    15661 cctcggccag ggagatgaac cggtccggcg acatcgtccc ccggtcgggg tgacgccacc
    15721 ggacgagtgc ttcgcaaccc agcggccgcc ccgtcgccag ctcgacctcg ggctggaagt
    15781 gcaggacgaa ctcgttgcgg tccaccgcgc cggtcaggtc ccggtcgagc tcgttgcgct
    15841 cgctcacctc ggcgcgcatg cgctcgtcga agatggcgac gcggttgcgc ccgcccgcct
    15901 tggcctcgta catggcggcg tcggcccacc gcagcaggtc ggtcggctca gcggtgtccg
    15961 aggtctcgac cacgccgatc gacacggtcg gccggaaggt ggtgccgttg atgtcgatcg
    16021 gttcgcggag cgagtcctgg agtcgggccg cgacggtggt cgcttctccg agggcgatgt
    16081 cggggagcag gacggtgaac tcgtcgccgc ccaggcgggc cagcgtgtcg tggggtcgga
    16141 gggtcgtgcc gagccgctct gccacgcggc acagcagctc gtcgccggtc ccgtggccga
    16201 gcgagtcgtt gacggtcttg aaccggtcga catcgaggaa cagcagcgcc aggcagcggc
    16261 cgtcgtcgtg tcggtcggcc cgctcgacca ccgccgcgct cagcaacgac agcagcatcg
    16321 agcggttggg gagtccggtg agggcgtcgt gggtggcgcg gtgggcgagc tcggcctcgt
    16381 ggtcgcggcg atgggtgatg tcccgggcca ccaggagcac ggactcgacc gagccgtcgg
    16441 cggcgaactc ggggacgaag gtcgactcgc accacacttc ggtggtgccg gatccgatgg
    16501 cgtactcgac ggtgaccggc cgacccttct tgaagacgat cgcgagggac tcgcgcagga
    16561 tgctggccga cgtccgatcg agtcggggcc aaccagcgcg catcacgacc cccgaggcgg
    16621 ccatctcctg gctggtgcgg accgccgcgg cgttggcgaa ggtgacgtgg tgggaccggt
    16681 cgaagcgcat cacggcgtcg gggaggctct cgaccaggga gccgaaccgg cgctcgctgc
    16741 gtccgagctg cgtcgcggtc cggtgcacct cgtcgacgtc gatcaggtgc acggtggaga
    16801 actggaactc gccggtcggc gtgcgactgg cgacggcgtc gacgcgatgc cagcggtagc
    16861 cggccggggt caggaagcgg acctcctggg cgctgcggtc cggtccgtcc gggcggagca
    16921 tcgccgccca ctgcggtgag gcctcggcca ggtcgtcggg atggatgcgt tcgagggcgt
    16981 tggagccggc gagctcgtcg gtgcggctgc cgaacatgtc gttggccgac tggttggccc
    17041 ggagcacggt cccgaaccgg tcgaggagca gtagcgggac aggcgcgccg ttgaagacgg
    17101 tcgccagctc ccggaccgtg tcgtcgaggg cgccttcggc gagggtacgg gccagcacgt
    17161 tggccaggag cgaggcgacg gccttgagcg cggtcacgtc cgaggaccgc caggtgaacg
    17221 gaccggtcac gacgtcgaag ccgatgacgc ccgccaaccg gccgtcgagg gcgaacggga
    17281 cctcgaggat cgcggtgatg tcctcgctct ggaggaactc gatgtcctcc tcccagccgg
    17341 gaccgagcag accgacgtcg tcgatctggt tgacctcgag cgcctcgagg cggtcctgct
    17401 ggcgggggcc gacagccatc ggcaggccct gttccatctc gaagcagccc gcgatcccct
    17461 ccgcgcacca ctcgtgggtc atggtcgtcg tcatggccga ccagtcgtag cggatgatgt
    17521 agctgcgatc cgcgccgacg aaggcgccga gctcggcgac cgtcgtgtgc aacgcggtgt
    17581 cgagctcggc ggtggcgacg ctgaggaagc gggcgaagag ccgatcgagg agctgttcga
    17641 acgcgacggg gcgttcgtcg atcacctcgt cccgctcgtc gttgcggtcg tcaccgcgac
    17701 gcgtccatcg caccgcgtca ggctagcgac gatccgcgcg agaacggtca ctcagagtag
    17761 tgcatgcctg gccggaacgg aggaacgtgt ctccatccca ctcacgtcgg tccgggctcc
    17821 agatcgggta ggccgagccc caacgtcgga gcctggagcc cgccgtcgat ctcgatcagc
    17881 ttgccggtca ggtagctgcc ggccggagag cacaggtaga ggacgcccgc cgcgatgtcg
    17941 tcgggtcggc cgagccgacg caggggcgtg gcctcctcca tggcggtgcg cagcgcgtcg
    18001 tcggtcagca ccgtgtcgag cgcggaggtg gcgatcgagc cggtggcgat cccgttgacc
    18061 cgtatgtgcg gcgccagatc cgccgccatc aaccgggtca ggtggctcag cgccgccttc
    18121 gctgtgccgt aggccgcgaa accccggtcg ctcatccggc cgatcgccga ggagatgttg
    18181 accaccgatc ctccgccccg cgccagcatc ggcgggaccg ccgacttggt cagctcgagc
    18241 gcagtggtca cgttgaaccg gaacgcccgc tcgaagctgg cgacgctggt ctcgagcagc
    18301 ggccggggga acgatccccc ggcgttgttg atgacgatgt cgagaccgcc gagccgttcg
    18361 gtcgcgacgg cgacgagctc gtcgaggcgg tcgaggtcca tgacatcgaa ggtcaccgac
    18421 tcggcccgag ccccgagcgc gcgggcctcc tcggcgaccg cgtcgacctg ctcggcggtg
    18481 cgggccgcga cgacaacgtc tgcccccgcc tcggccagcg ccagggcaca ggcccgcccg
    18541 atcccccggc ccgctccagt taccagcgcc acctgaccgt cgacgcggaa gcggtcgagc
    18601 acggtcatcg atccgccgtc ccggccccgg ggtccgtcga cccgggttcg tctccggtca
    18661 tcccggacgc gtcggcggac tcccgggccg cccagcggaa caccccgctg gccatcagcg
    18721 acgcctcggc gcgggcccgg gcgccgaaca cctcggagcg gaccgcgtgc acacccgagt
    18781 cgccgggtgc cgcctgggcg gcgagctcgg ccaggtgtcc ggccagccgc aggtccccct
    18841 ccgcggccag cgcccgtgcc cgctctgcca gcaccgaggc tccaccggcg agtgccgcga
    18901 gctcggcggc gaccgccgcg tccggagcag gcttgaggcg ggccgggttg ccgtcgtacc
    18961 agccgccgta gagccgccag atgttgtgga ccacgaactc cggctcgtcg tagatcggtc
    19021 gcaaccaggg cagtccggcc aactcgtcgg gaacccggac cgtgtggacg atgtcgtcga
    19081 gccgggcgcc cgcgttcatc atctcgaggg tctgggaagt gatcgactcc aggtactcgg
    19141 ccgcgctgcc gaggacgcgg gcgatgtcgt cggagccggc gatgggcgga ccgtgtcccg
    19201 gcatcagcag ctcggcgccc aggtcggcca tgcgacgcag cccggccgcc cactccatcg
    19261 ggtagcgctg caccttctgc gggttgccgc agttgggcga cacccagatg aacatgtcgc
    19321 cggcgcagag gaccttccgg tccgcgatcc acgcccaggt gtggtcgtcg gtctcgccgc
    19381 gggagtggtg cagctcgacg gtccgggcgc cgacggtcgc ggtggccgac ccccggtagg
    19441 tccggtccgg gtagcggtac tcggtgggga accacggttc gggcaggcgg aactggcgct
    19501 ggttgatcac gccgttgtag ccggcggtca tccggtaccg gtcgaagcgg tcgacgacgc
    19561 gctcgtgggc gacgacctcg ggggcgagtc gtccccgctc ggccgcctcc tgctcgaacc
    19621 aggtgatgcc gccgacgtgg tcggtgtggc cgtgggtgta gaccgccgtg ctgagcggcg
    19681 cgtcggtcca ggagcggacc agttcgtgga cggtgcggcc gtggaacgcg ctgctgacgt
    19741 cgatcagcag cagctcgtcc tcgaccacgg agcggccggc tccggcgggg gcggtgaagg
    19801 ccgccacgtt ggagaacgct gcgacgaagg cgatgtcgtc gtcgaggacg tgcagctcag
    19861 gggcctcgtt gaggccgatc gatccgaact cgccgggcgg agcgtcaccg gcgatgactc
    19921 gggcggactt ctcgagcagg tcgacgttca cgtcgccacc ctacgcagcg cacgcggcgc
    19981 cggttcggct gtgcccgacc ggaacccctc gattctcatg acatctgagt accgcggttg
    20041 tcgtgacttg tcgtcagaaa ccgggttggg ggggtgcggt caggtggggg cgggttcggc
    20101 cagcagcgcc gagaggtggt ggcgccggtc acccagcgtg cgcaccagga actgcgcccg
    20161 ggtaaggaac cggtgcgcgg tgtgctccca cgtgaagccg atgccgccgt ggttctggat
    20221 gttcacctgg gcgttggtca ccgcggcctc cgacgcgacc gtccacgccg cctgcgcgtg
    20281 gaacgcgccg tcggtccgtc cgtcgacgac ggccagggcg gcgtagcggc tcagcgacgt
    20341 ggccgcctcg gcccgggccg ccatgtcggc gcagcggtgc ttcaccgcct ggaacgatcc
    20401 gatgggccga ccgaactgct cgcggtcctt ggcgtactcc accgactggg ccgcggtcgc
    20461 cgtcgcgatc ccggccagct gcgatgcgat caggatcgtg gcccgcagcg ccagctcgga
    20521 gctgtcggcg accgacgccc gagcggtcac gcccgcgaga tcgaccacgc cgagcggggt
    20581 gagcaggtcg atggacggct cgaccgacgc ggcgacggcg tcgacgaggg cgagtccggc
    20641 ggcggggtcg gacccggcga ccaggagcac gtccgcaccc ccgtggtcgg tcacccggaa
    20701 ccggcccgac acctcgtcgc ccaccgacgc gcgtgcgtcc tcggcctcgg ccagggcgac
    20761 gagcacgtct ccggccagga tggccgcggc cagttcgccc tcaccggcgg tggcggccag
    20821 gcgggcgccg agcaccgtgg ccaggaacgg cccgggggtg gcgtgggcgc cgagctcggc
    20881 gaacagcaac gactcctcga ccagggtgta gccgaccccg ccgagctcct cgtcgagtcc
    20941 gagtccgaac cagccgagct cggcgcactg gcgccacagg tcgcggtcga ccacctggac
    21001 cgatccgtcg ttcgcggcga ggtcgttcag cgcgaaccgg cgttccagct gggcccgtac
    21061 cgtggtgacg atctcttcct gttcaggcgt gggcagaagg tccatgtcgg tccccgatca
    21121 cgcgcgcggc aggcccagga cccgctcgcc cacgatgttg cgctggatgt cggtcgtgcc
    21181 gccgccgatg gtgtaggcga agctctgtag gtaggggccg gtccacacgt cgccgtcgac
    21241 cggggtgagg cgcagggcgt cggccccgat gatgtcgagg gcgagctggt acacacgctg
    21301 gtgcagctcg ccgtggaaga agcggatcat cgacccctcg gctccgggca tgccggtgcg
    21361 ggcggtgcgg ctgatcccgg cgatggtcat ggccttcagg gcgtcgacct cggctcgggc
    21421 ggtggccagg cggcgggcga cctcctcgtc gccgatgagc ggacggccgt gggcgtcggt
    21481 gcgctcccgt gcctcgccga tcagccgctc gatggtcgag gtcagctcga cctgatccgc
    21541 catgaacgcg gttccccgct cgaagctcaa cgtcgacatc gccgtgcgcc acccgttgtg
    21601 cagtccgccg acgacgttgg tcaacgggat gcggacctcg tcgtagaaga cctcgcagaa
    21661 gtcggtgacg tggctcatcg tgcggatctc acggatctcg atgcccggcg tggtcatgtc
    21721 gcacacgacc cagctgatgc ccttgtgctt gggggcgtcc gggtcggtgc gcaccagcag
    21781 ctcctggagg tcggccacgt gcgcgtagct ggtccaggtc ttctggccgt tgaccaccag
    21841 gtggtcgccg tcgatctcgg cccgacacgt caggctgccc aggtcgctgc cggcacccgg
    21901 ctcggagaag ccctggcacc acacgacctc gccgcgcagg atcgggggca ggtaggtcgc
    21961 cttctgctcg tcggtgccgt tgacgatgat cgtcgggccg ccgtggttgt tgccgacgaa
    22021 cgtgcaggag ttgttgaggg tgaacggcgg gttggcccgg gcgaactcct cgtaccagat
    22081 catctgctgg atgtcgctca ggccccgccc gccgaagtcg accggccagc tgatgccggc
    22141 ccagccgccg tcgtagaggg tgcgctgcca ggccaggtcg aaggcgcgca tctcctggcc
    22201 ctcgggcggg cgctcctcgg tgggcaggtt ctctcggagc caggtgcgcg cttcctcgcg
    22261 gaacgcgagc tcctcctcgg tcaggtcgag gttcatgggc cagaaggtac ccgcgcatct
    22321 gacggatcgt cagatgcgcg gacgccgacc ggggtccggc tcccgggtgt cagctgccga
    22381 cgcgggtgac cacgcaggtc accttctgca cgtcgtcgga tgcggagccg gtcacctccc
    22441 aggttcccga cgaggacgcg tcggtgctcc cggagtcgac cacgtcgttg ccgtcgctgt
    22501 tgcggaactc gacgtggacc ttggctgact cgtcggcgcc gtcgaccttg cccgccgcgg
    22561 tcaacgagcc gtcggcgtcg atgcggcact cgcggatggt cgcggtcgga ccggttccgg
    22621 atccggcgat gaggaaggcg accgctccgc cgacgaggag cagcacgacg acgagcaggg
    22681 cgatcaccag gccggccttg ctcttcttct tgggtgcctc ggccccgacg ggggcggcgt
    22741 agcccggagc gacacccgga gggccgaccg gcggggcccc gtagccgggt gcccccgggg
    22801 cggcataggg cggcgtgcct ccgggcccca tcggcgcgta gccgggggcg gcacccggcg
    22861 ctccgggcgg cgcgggcggt gcgggggtca tcggtgccac gggcggcacc gggacggtgg
    22921 ggttggtcac ctgcgtcggc gcctcgatcg gcggaggcgg ggtgggg
//
LOCUS       DAOQOD010000029        22706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_766_length_22706_cov_11.848351, whole genome shotgun sequence.
ACCESSION   DAOQOD010000029 DAOQOD010000000
VERSION     DAOQOD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 22706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22706
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_22706_cov_11.848351"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..636)
                     /locus_tag="PLZ72_04735"
     CDS             complement(340..636)
                     /locus_tag="PLZ72_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNA35641.1"
                     /translation="MTDGFAVEVDEGVIVVSGDLDAQTSGRLDDVISGRLDIGVDELV
                     FDMGGLEFIDSSGLRSLVLARGDGDRRVVLRSPSASVVRLLDITGLLDVFTIEH"
     gene            771..842
                     /locus_tag="PLZ72_04740"
     tRNA            771..842
                     /locus_tag="PLZ72_04740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:804..806,aa:Gly,seq:tcc)
     gene            1008..1781
                     /locus_tag="PLZ72_04745"
     CDS             1008..1781
                     /locus_tag="PLZ72_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35642.1"
                     /translation="MIPSPSDRTEVRSALVLGATSDIAVATLAELHRRGPVRAVLAAR
                     DPDAATAAVAAAAPGLSTEALAWDALEVEGHRPLFERAATTLGAIDVVLCAVGLLGHH
                     AGMSMDPVDVDHMIRTNFAGPAAALAAAGQHLVEQDHGTIVVLSSVAGVRARRSNYVY
                     GSTKAGLDLFAQGLSDSLVGTGARCVVVRPGFVRSRMTEGLDPAPFSTDPATVARATV
                     DALERPGRDVVSVPRVLGPLFGVLRVAPRGLWRRIAGDR"
     assembly_gap    1786..1885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1888..2274)
                     /gene="msrB"
                     /locus_tag="PLZ72_04750"
     CDS             complement(1888..2274)
                     /gene="msrB"
                     /locus_tag="PLZ72_04750"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNA35643.1"
                     /translation="MTDVDPDELRDRLDDLQWRVTQESGTEQAFTGVYWDCHDQGTYR
                     CVVCDTPLFRSDVKFESGSGWPSFWEPMEPGVIEEVSDRSHGMVRTEARCARCGAHLG
                     HVFPDGPQPTGLRYCMNSAALNLERD"
     gene            complement(2261..3499)
                     /locus_tag="PLZ72_04755"
     CDS             complement(2261..3499)
                     /locus_tag="PLZ72_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA35644.1"
                     /translation="MRTSGVRLGRVFDIEISADLGVVMFAGLLTWILASSILPASVAG
                     LPAGAYWSVAALGTLLFLGSLLAHELGHAVVARRNDIEVLGITLWLFGGVAQLRSDAR
                     TAGAELRIALAGPAMSLVVGALSLAAAVGLRSVGVPEIYVVALGWLGLVNVVLAVFNL
                     LPGAPLDGGRVLAALIWMVRGDRLRARVWAARAGRAVAILIVAAGAAEVFVLRSSSGL
                     WTIMVGWFLMTAARAEESRYRSEEALGSMPVADAMDRDPASIRSWSTVADAASGALAD
                     RGTWAAVPVVDADHRIVGVMTLARLRRLPAERWATTTVADVMLPVSQLPTATPGERVV
                     SVLDRLRIQSGGIAVVLDQGRLVGLLTPEGIDRAISFGRRATAGPSPGGRTLIAVPPP
                     VPDGVPVQRWEPPGGADDRR"
     gene            complement(3830..4609)
                     /gene="yaaA"
                     /locus_tag="PLZ72_04760"
     CDS             complement(3830..4609)
                     /gene="yaaA"
                     /locus_tag="PLZ72_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNA35645.1"
                     /translation="MPEPPLILIPPSEGKAPGGSGPAWAPSTMAIDLDGRRLAVMAAL
                     RSAMRRNEAQRQKLLGVKGDALAAATAANRTVAESPTMAAAQRFTGVLYDALDLPSLS
                     AAARRRADSSVLVLSAVFGLVVPSDPIPDHKLKMSVSIGRMGKLSTWWRAPISAALSE
                     RAGGRAVWDLLPLEHSAAVDLGGVPRTTVVFLEPNARGELVAVAHWNKLLKGALVRHL
                     LENPGTDAADLADWEHPEGFVLDADRTVVDGALTTLFMVRR"
     gene            complement(4667..4740)
                     /locus_tag="PLZ72_04765"
     tRNA            complement(4667..4740)
                     /locus_tag="PLZ72_04765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4704..4706),aa:Arg,seq:tct)
     gene            4785..4862
                     /locus_tag="PLZ72_04770"
     tRNA            4785..4862
                     /locus_tag="PLZ72_04770"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4820..4822,aa:His,seq:gtg)
     gene            4945..5017
                     /locus_tag="PLZ72_04775"
     tRNA            4945..5017
                     /locus_tag="PLZ72_04775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4978..4980,aa:Lys,seq:ctt)
     gene            complement(5095..5526)
                     /locus_tag="PLZ72_04780"
     CDS             complement(5095..5526)
                     /locus_tag="PLZ72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNA35646.1"
                     /translation="MLATLAQDFETTTSSGGGFAAFGILMVIYLALFVVFLIGYWKVF
                     TKLGLPGWMGIIPLVNVYMLFKARGQHEPVVWLILSLIPCINIIAMWMLANDTAELFG
                     KELGWKIFLFLIPGISHIVLGFGSAEVDRSALAPGVGFNAA"
     gene            complement(5674..6825)
                     /locus_tag="PLZ72_04785"
     CDS             complement(5674..6825)
                     /locus_tag="PLZ72_04785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA35647.1"
                     /translation="MDDGGGVPIGGFCAERFDAVRVAFADNFVEQGELGAAVCVIHRG
                     EVVVDLHGGWADPARTRPWAADTITDFYSVGKGLLSLLLLRLVDRGEVGLDDPIASVW
                     PEFAAGGKASATVRHALTHRAGVPAIRRHLNDDDLFDWSTMTGTLAATEAWWPPGEQL
                     AYHVNTFGHLVGELVRRIGGAGPGEALAALAGPLGADVHFGVPSDQQHRCADTVWDEA
                     FRIPAFDLDRLEGLNLLHALAHFNPPGYSSVGVMNTPRWRSLPLGSTSGHGTAAGVAR
                     IYSALLDDDRLLSPALLRAATTVQAVGHCPILDDELSYGLGFATTTDRRRLGPNAGAF
                     GHYGTGGSLGFADPVDGIAFGYVMNHVIPRWQSTRNRALIDAVYRSLHR"
     gene            complement(6840..7652)
                     /locus_tag="PLZ72_04790"
     CDS             complement(6840..7652)
                     /locus_tag="PLZ72_04790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNA35648.1"
                     /translation="MVSSALAPESGALYGRETISWDVNRRWFPLIGGPRAAILQVCHP
                     KVAAGVANYSEFRTNPIGRLDHTLDAMLTISFAAPERRDAMLKQLRAIHARVRGTTAD
                     GEKYRANDPELQYWVLATLVDTTFEVERRYVGEMRRADRERFFEESKLMAGAFGIPDE
                     LIPEDLAAFRSYMAERFATLEPTDESREITRVLMRPGLRWIPDQSFVPLNWVTAELLP
                     RRMRRLLDVGELNPAELSAVRGARLMARTTLPRLHGILSVNPWNQRVLRTAA"
     gene            complement(7750..8616)
                     /locus_tag="PLZ72_04795"
     CDS             complement(7750..8616)
                     /locus_tag="PLZ72_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNA35649.1"
                     /translation="MTRRSWPRRAATTPLLALAVVAVLAGGCGSDDTPSGAGSIPTDC
                     GPAPEPTPLRAEVLAEHPHDPTAFTQGLVVVDGALYESTGRRGHSSVREVDLDSGRVL
                     RSTDLDPELFGEGLTAVGDDRLVQLTWTEGRALVWNRSELTAVGEHRYDGEGWGISTL
                     DDGRLVMSDGSDTLTVRDPDDFSVLERWQITRADGAADRLNELEWDGERLWANRWQTD
                     EIVRIDLRCRRVDGVLDASALTARAATAAAGRPIDVLNGIAHLPDSGDLLLTGKYWPL
                     LFEVAVTEDARR"
     gene            complement(8613..9863)
                     /locus_tag="PLZ72_04800"
     CDS             complement(8613..9863)
                     /locus_tag="PLZ72_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35650.1"
                     /translation="MDPGPDPTPPPEPPPEPTPTEPVISVDRLVVGGAALGHEDSGRV
                     ALVRGALPGETVRIRVLRERPRSVDAEVVEVIAASPDRVEPPCPELARGCGGCDLQHA
                     APAAQRSLKAAIVSDALARSVRAGRFDAVPVEVGPAADPWGYRSTVRGGVDGGRFAFH
                     RHHSDDLLAVADCAVAADPVAEVIRDGRFPDGVDVTIRAGLRTGERLVILDTGAAQDA
                     VVPDGVRVIGGVELSAGQRAWFHEEVAGVRLRISARSFFQSGPSGAEALVDSVRRAVG
                     TLGSSDRLADLYGGVGLFAAVLGAERPIVVERSAASVADARVNLADRDATIVRSAVER
                     WRPSPVNVVVADPARSGLGRDGVRAVAATGAGRVVLISCDAAAMVRDLEMLTASGYRA
                     TRIEVLDLFPHTHHVEVVSTLELV"
     gene            9929..10609
                     /gene="rpe"
                     /locus_tag="PLZ72_04805"
     CDS             9929..10609
                     /gene="rpe"
                     /locus_tag="PLZ72_04805"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNA35651.1"
                     /translation="MSAPQIQIVPSVLPADFARLGEECQSLEKAGVDRIQWDVMDGVF
                     VPNLTFGPDVIAACRPLVDVPFEAHLMVVEPDALLPRYVEAGCEVLIVHAEACRHLHR
                     TLASIRDLGASPAVALNPATPVDTLRHVLDLVDEILVMTVNPGFGGQAYIDTMEPKIA
                     EVRAMIEMSGRDIDLEVDGGIGPATVSGAAGAGANVLVAGSSLFRDPEGRAHAVSEIR
                     SIATAAFA"
     gene            10610..11077
                     /locus_tag="PLZ72_04810"
     CDS             10610..11077
                     /locus_tag="PLZ72_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35652.1"
                     /translation="MLLTPMPVRSRRRNLAGAVLAVTAVVAVSTGCGDGRPAFCDDLA
                     RAADMDALSAALRAQDLDRARTAAREFSDLADGAPEDLRPDMQDLAGAVSEIVDLLSA
                     ERNAMPGAGHEGQGDAAVVERQRDELNERLDDLAVTSSRVERWASRECGLDLD"
     gene            11335..11853
                     /locus_tag="PLZ72_04815"
     CDS             11335..11853
                     /locus_tag="PLZ72_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNA35653.1"
                     /translation="MDRPSLNSLYEDRDSSAEQVTTTTAARRGGVRRAGVAVMLAGVL
                     AMLVLVGSSCTRNAYAFDSAAQVNATRSSSGLSKLAIDDTLVAKAQAWAERMASAGKI
                     SHSQLTDAAGNNWSVLAENVGMASSISQMHSLFMNSPSHRANIVNGSFDRIGTGVAES
                     GGRLFVVQVFAG"
     gene            complement(11946..12518)
                     /locus_tag="PLZ72_04820"
     CDS             complement(11946..12518)
                     /locus_tag="PLZ72_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNA35654.1"
                     /translation="MQAGNGMEPSADIYNRLLKNRIVFLGSEVNDTVANFITAQLLFL
                     EGEDAEKDIWLYINSPGGSVTAGMAIYDTMQFITPDVGTICMGLSASMGQFLLCAGAP
                     GKRYALPHARIMMHQPSAGMQGQASDIAIQAEQLKYIKDLLAERIAFHTGQTKDQINE
                     DSDRDRWFTAEGAKEYGIIDHVIVRRGEMR"
     gene            12670..12753
                     /locus_tag="PLZ72_04825"
     tRNA            12670..12753
                     /locus_tag="PLZ72_04825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12704..12706,aa:Leu,seq:tag)
     gene            12774..13634
                     /gene="purU"
                     /locus_tag="PLZ72_04830"
     CDS             12774..13634
                     /gene="purU"
                     /locus_tag="PLZ72_04830"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNA35655.1"
                     /translation="MAATAVLLLSCPDRTGVVAAVADFVWRHGGNIVDAEQHTDDVDG
                     VFFQRVEFELDGFAFERDDLPKAFWAVAERFDMDVRVHFSDERPRLAILASKASHCLE
                     DLLNRWRAGELPAEVALVASNHPDHRALAEFSGVPFHHLPVGDGPDGAARQEAAMIEL
                     LEREQVERVVLARYMRILSPTFVERFPSRIINIHHSFLPAFVGAQPYRQAHERGVKVI
                     GATAHYVTAELDEGPIIDQDVARVSHRDSVAELARKGRDLEVAVLARAVRADLEHRVL
                     PFGNRTVVFH"
     gene            13688..14485
                     /locus_tag="PLZ72_04835"
     CDS             13688..14485
                     /locus_tag="PLZ72_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNA35656.1"
                     /translation="MPEGHIIHRLAREIRSTLGGAPVLASSPQGRFADGAAAIDRHRL
                     VRTDAWGKYLFCDFGTGEILHVHLGLIGKFRRRATPPPDPVGVVRLRLVGQGTTWDLS
                     GPTRCELITPEEQDRIVAGLGADPLRRGADVATMRDRFARSGRPVGAVLLDQSIVAGI
                     GNVYRAELLFLCGIRPDRAAASLTDDEFDELWRRTVELMRVGTRLGRIVTTEPDEIGR
                     SRGRMGDEDRLYVYHREHCRRCGGPIATAEIGGRQIWYCPTCQPATA"
     gene            14571..15596
                     /locus_tag="PLZ72_04840"
     CDS             14571..15596
                     /locus_tag="PLZ72_04840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNA35657.1"
                     /translation="MKVRDLPTPSLVVDVDALDANIATMAAARPGRTLRPHVKAFKST
                     ELARRVAELGGHQAFCCATLREMEGMADAGLGDDLLLANETLDAPRLRHLVDRGDARV
                     TVAVDSPETVEVAAHAGAEVLVDVDVGLPRCGCRPEDAGRLAELARTRGLNVRGVMGY
                     EGHVVGDPDRGRRAEKVAESMALLRRAHDDVGGDVTSAGGTGTYDLHDWAGEVQAGSY
                     LLMDTHYAALGLPFRQALRLEVTVISTSPERGWGVVDCGLKSLGMDHGNPTISGWNVF
                     FCSDEHTTFLADATGDSEGRALPAVGARLGVLPAHVDPTVAYHDRLHLVRGDEVIDVW
                     PVDLRGW"
     gene            complement(15612..16253)
                     /locus_tag="PLZ72_04845"
     CDS             complement(15612..16253)
                     /locus_tag="PLZ72_04845"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNA35658.1"
                     /translation="MTGRLVVASANAHKAAELQDLLVSLGIGLDVVARPADLAVPAED
                     APDFIGNARIKAAAVAAATGEWALADDSGLEVDALDGAPGVRSARFAGDEASDADNVA
                     LLLDRLVGLGAVTAAERRARFRCCIVVQSPPDADGSVIELVADGTVEGHIAGAPRGNG
                     GFGYDPVFVPDDPVGDASAPGRTFAEMSGIEKASISHRGRALRAIAPQLARLP"
     gene            complement(16258..16995)
                     /gene="rph"
                     /locus_tag="PLZ72_04850"
     CDS             complement(16258..16995)
                     /gene="rph"
                     /locus_tag="PLZ72_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNA35659.1"
                     /translation="MRPDGRAPDELRPITFERDYTAYAQGSVLVSCGDTKVLCTAMVD
                     DDVPRWMRNTGKGWVTAEYSMLPGSSPERIRRETKGPKGRTQEIQRLIGRALRSVCDM
                     KALGERQILIDCDVLQADGGTRTASISGAYLALHDCLTRVVQAGLLSEVPLYDELAAI
                     SVGVVDGVPVLDLPYAEDSTAETDMNVVMTGKGLFVEVQGTAEGAPFSRDELDSLLGL
                     AEGGIRRIHALQRKYLAEAPTPRPIGR"
     gene            complement(17032..17850)
                     /locus_tag="PLZ72_04855"
     CDS             complement(17032..17850)
                     /locus_tag="PLZ72_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA35660.1"
                     /translation="MDAAMVTDLGTDVVPDPSRLAVTVLGCGGTFAGAGNACSGYLVR
                     GGGTAVLLDCGPGVLSSLQHHLEPTELDAVVISHSHPDHWGDVPVMRNALRYVLGHHG
                     VPLFTTADTLAILDGVFHDGLGDAFAPSVITDGADVTVGALRVRTSRTAHPPETMGIR
                     VDMGDVDLGDGASTRSIAYSADTGPAWSLGRLGDGIDLGICEATWLAADGFDVDDLHM
                     TAEQAGRLARADGVSQLLITHVPPTGSVELAVAEASAAYGAPVDPARIHHTYLA"
     gene            complement(17878..18702)
                     /gene="murI"
                     /locus_tag="PLZ72_04860"
     CDS             complement(17878..18702)
                     /gene="murI"
                     /locus_tag="PLZ72_04860"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNA35661.1"
                     /translation="MRDGPIGMFDSGFGGLTVARAVIDLLPAEDLVYLGDTGRYPYGP
                     RPLDEVARFAHQIARELVERRGVKLLVVACNTASAAALDSLRDRFEVPVIGVIEPGVT
                     ALVQATVTGRVGVVGTVGTINSGAYQRAVAAASDDAELIALACPGFVEFVERGEFDSE
                     QVHVLARALLEPAREAKVDTLLLGCTHYPYLARTLNDVMGRDVVLVSSADETAFEVRR
                     VLSDLGLARPATGADASGRASCGRAEFITTGDVNWFEELGRRLLGPELDRAEKLSW"
     gene            complement(18759..19706)
                     /locus_tag="PLZ72_04865"
     CDS             complement(18759..19706)
                     /locus_tag="PLZ72_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNA35662.1"
                     /translation="MTVYSSVLDLIGNTPIVDVSQLSPNPRVRILAKMEGQNPGGSVK
                     DRIALAMILDTEADGTLSPGATILEPSSGNTGIALAMISRIRGYHLKIVLPENVSIER
                     RQLLEVYGAEIIPSPGEEGSNGAVRRAQALSEEHPDWVFLYQYGNEANPQAHYRTTGP
                     EIWSDVPDITHFVAGLGTSGTLLGCGTFLKEQNPDVQVFAVEPPSGELVEGLRSLDDG
                     YIPPVFDKWGGYDLLDGKSIVRPRESLEYTRRMAEVGIFSGISAGAALAGAVKVASRI
                     ESGVIVFIVSDYGWKYLSTGAWTLDLDEAAANAEKVIYF"
     gene            complement(19784..20194)
                     /locus_tag="PLZ72_04870"
     CDS             complement(19784..20194)
                     /locus_tag="PLZ72_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNA35663.1"
                     /translation="MLAITPELHDEMAAHALRGLPLEACGLLVGDPDDPQQRVIRFVP
                     CRNAAESAVVYTVHPVDHLRADRAAEDDGLAIVGVMHSHTHTDAYPSPTDVASAPDPS
                     WHYVIVSLRDDEPTLRSYRIVDGTIAEERVDLVR"
     gene            20275..20748
                     /gene="smpB"
                     /locus_tag="PLZ72_04875"
     CDS             20275..20748
                     /gene="smpB"
                     /locus_tag="PLZ72_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNA35664.1"
                     /translation="MKPTGTQIVATNRQARRNYTLGDTFEAGLVLKGSEVKSLRESKV
                     QLTDAWASVDGDEAWLNGLHIAPYTLAQAHSGHEPVRRRKLLLHRGEIDRIRMRMQVE
                     RLALVPLSLYFKDGRVKVELALGKGKNVVDKRQDIAKRDADREAAREISRARRGD"
     gene            21138..21452
                     /locus_tag="PLZ72_04880"
     CDS             21138..21452
                     /locus_tag="PLZ72_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35665.1"
                     /translation="MLVTGDSAGFAASFPAPGPRDRPPYITRIDPAAVIGCGVLHRPG
                     FTPVDLADEGAGGFGGCATQYRSELAGLARRPNWMVMFSGGWEHLPWIPPGDRAAPRS
                     LT"
     gene            21502..21792
                     /locus_tag="PLZ72_04885"
     CDS             21502..21792
                     /locus_tag="PLZ72_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35666.1"
                     /translation="MSIGTRTAFVSWACPSGVAPTRTGDYARWYDQILREAASRARGA
                     IVIEPTDRVCVGGDPGAAATPEKDWAFAGTQHPHDRRWLWNEWLGPILLANS"
     gene            21985..22167
                     /locus_tag="PLZ72_04890"
     CDS             21985..22167
                     /locus_tag="PLZ72_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNA35667.1"
                     /translation="MVQYEFLKAWLSAQARTERGASLVEYALLVALIAVVCIAAISFI
                     GTSASNKFSQIGSSIQ"
     gene            22315..22706
                     /gene="ssrA"
                     /locus_tag="PLZ72_04895"
     tmRNA           22315..22706
                     /gene="ssrA"
                     /locus_tag="PLZ72_04895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3400 a         8056 c         8010 g         3140 t
ORIGIN      
        1 tctgcgcgga cagcacaccg atgcgatcat ccaactcgcg ccgacgcgac tcccaatcac
       61 caaccgcgcc cacaaccact ccaccagcag cagcgccagc cgcttctggc cgctccgagg
      121 tgatggtgcc ggtgtccatg cccaccatca tatcgaacac gtgttcgatc ctgcaaggcc
      181 tgggcccgga ttcgtcgcag acggttcggc ttcgaacacc cgcatgaacc caaggcgaaa
      241 ggcacctttc gcgaatacga aacccacccc atcggtggat ccaggctgaa ccgccccgga
      301 cccaaccgtc ccgacgcccc gaggccggga ccggtccgtt cagtgctcga tggtgaagac
      361 gtcgagcagg ccggtgatgt cgagcaggcg gaccaccgag gccgacggcg agcgcagcac
      421 gacccggcgg tccccgtcgc cccgggccag gaccagggag cgcagtccgc tggagtcgat
      481 gaactccaac ccacccatgt cgaacaccag ctcgtcgacg ccgatgtcga gccgaccgga
      541 gatgacgtcg tcgagccgac cggacgtctg ggcgtcgagg tcgccgctga ccacgatcac
      601 accctcgtcg acctccaccg cgaacccgtc cgtcatcggg accaaaccta ttggtcccgg
      661 cggtcgagtt ccaccgtcgg acccgacaag ggagcagacg accgacccgg gacggcaccg
      721 ggattcccgg gcctgcgcta gcgtgaccgg tccctgctcc ggcagggaac gcgggtgtag
      781 ctcaatggct agagtcccag tcttccaaac tggcgatgcg ggttcgattc ccgtcacccg
      841 ctctcatgct ccggcctggt cgtggagctc gaccaggaag ggcgtcggcg cggcgtcggt
      901 ccgggaccgg tacttcaggt ccaggccggg accagggccg tggtcgcacc ccggtcaccg
      961 gatcgtgagg agatcgtgaa gatccgtcgc ccgctacggt cctccgggtg atcccatcac
     1021 cttccgaccg caccgaggtc cggtccgcgc tcgtgctcgg ggccaccagc gacatcgcgg
     1081 tggcgaccct ggcggagctg caccggcgcg gaccggtgcg ggcggtgctc gccgcccgcg
     1141 atcccgacgc ggccaccgca gcggtcgctg ccgcggcccc cggtctgtcg accgaggctt
     1201 tggcgtggga cgccctcgag gtcgagggtc accgtcccct gttcgagcgg gcggccacga
     1261 cgctgggggc gatcgacgtc gtgctgtgcg ccgtggggct gctcgggcac cacgccggga
     1321 tgtcgatgga tccggtcgac gtcgaccaca tgatccgcac gaacttcgcc ggaccggccg
     1381 cagctctcgc ggcagccggc cagcacctgg tcgagcagga ccacggcacc atcgtcgtgc
     1441 tgtcgtcggt cgccggagtc cgggcccgac ggtccaacta cgtctacggc tccaccaagg
     1501 ccggcctcga cctgttcgca cagggcctgt ccgactcgct ggtcggcacc ggggcccggt
     1561 gcgtcgtcgt gcggcccggc ttcgtgcgct cacggatgac cgagggcctg gacccggcgc
     1621 cgttcagcac cgacccggcc acggtggccc gagccaccgt cgatgccctc gaacgacccg
     1681 ggcgcgacgt cgtgtcggtc ccccgcgtcc tcggaccgct gttcggcgtg ctgcgggtgg
     1741 cgccccgggg tctgtggcga cgcatcgcgg gtgaccgctg aacgannnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnggtca gtcccgctcc aggttcaggg ctgcgctgtt
     1921 catgcagtac cgcaggcccg tcggctgcgg accgtcgggg aacacgtggc cgaggtgggc
     1981 gccgcaacgg gcgcaccggg cctcggtgcg gaccatgccg tgggagcggt ccgacacctc
     2041 ctcgatcacc ccgggctcca tcggctccca gaagctgggc cagcccgagc ccgactcgaa
     2101 cttgacgtcg ctgcggaaca ggggcgtgtc gcacacgacg catcggtagg tgccctggtc
     2161 gtggcagtcc cagtacacgc cggtgaaggc ctgctcggtc cccgactcct gggtgacccg
     2221 ccattggagg tcgtcgagtc ggtccctgag ctcgtcgggg tcaacgtcgg tcatctgctc
     2281 ctcctggagg ttcccaccgc tggacgggga cgccgtcagg caccgggggc ggcacggcga
     2341 tcagcgtacg gccgccgggg gacggccccg cggtcgcccg tcggccgaac gagatcgcac
     2401 gatcgatccc ttccggtgtg agcagcccca ccaggcggcc ctggtcgagc accacggcga
     2461 tcccgccgga ctggatccgg agccggtcga gcaccgagac gacccgttca ccgggggttg
     2521 cggtcggtag ctgcgacacg ggcagcatca cgtccgcgac ggtcgtcgtc gcccaacgct
     2581 ccgcgggcag ccgtcgcagc cgggcgagcg tcatcacgcc gacgatccgg tgatcggcgt
     2641 cgaccaccgg aaccgcggcc caggtgccgc ggtcggccag tgctccggag gcggcgtcgg
     2701 ccacggtgct ccagctccgg atggacgcgg ggtcgcgatc catggcgtcg gccaccggca
     2761 tcgacccgag cgcctcctcg ctgcggtagc ggctctcctc ggcccgggcc gcggtcatga
     2821 ggaaccagcc gaccatgatc gtccacagac ccgagctgct gcggagcacg aacacctcgg
     2881 ccgccccggc ggcgacgatc aggatcgcga cagcccgacc cgctcgggcc gcccacaccc
     2941 tggcccggag gcgatcgccg cggaccatcc agatcagcgc ggccagcacc cgaccgccgt
     3001 cgagcggagc accgggcagc aggttgaaca cggcgagcac gacgttgacg agacccaacc
     3061 agcccagcgc gacgacgtag atctcgggaa caccgacgct gcggagtccg acggccgcgg
     3121 ccagcgacag cgctccgacc accaggctca tggcgggacc ggccagcgcg atccgcagct
     3181 ccgctcccgc ggtccgcgca tcggagcgga gctgggccac gccgccgaac agccagaggg
     3241 tgatgccgag cacctcgatg tcgttgcggc gggccaccac ggcgtgcccg agctcgtggg
     3301 ccagcagcga gccgaggaac agcagcgtgc ccagagcggc gaccgaccag tacgcgccgg
     3361 cgggcagacc ggccaccgag gcggggagga tcgacgacgc caggatccac gtcagcagtc
     3421 cggcgaacat caccacgccg aggtcggcag agatctcgat gtcgaagacc cgtccgaggc
     3481 ggacacctga ggtgcgcacc gcactcctcc ctgtcgaacc gcaaactacc ccggcccgag
     3541 ttgggccggc cgggggtcga cggcgagggc tgagaaggaa ctccagatgg caggagctcc
     3601 gggtgggaga gcgagtgggt cgccctcgac ctgtccccgg acagcaacga acccccggcc
     3661 ggagagtagg agggaaggga agaaccctac ggccgggggt cgttgttcat tgtttctacc
     3721 ccatccgaga ctgttcgcgc tcgtcaacgt ggacgtgaag gtgttcacat cgggtgaact
     3781 ttcctcaccc ctccgcggca tcgggtccgc tcccggatcg gtcccggggc tagcggcgca
     3841 ccatgaacag ggtggtgagg gctccgtcga cgacggtgcg atccgcgtcc agcacgaacc
     3901 cctccgggtg ctcccagtcg gccaggtcgg cggcgtcggt ccccgggttc tcgagcaggt
     3961 ggcggaccag ggcgcccttc agcagcttgt tccagtgcgc aaccgcgacc agctcgcccc
     4021 gggcgttcgg ttcgaggaag accacggtgg tccgcggaac gcctcccaga tccaccgcgg
     4081 cggagtgctc gagcggcagc aggtcccaga cggcccgccc gccggctcgt tcggacaacg
     4141 cggcggagat cggggcgcgc caccacgtcg agagcttccc catgcgtccg atcgacacgc
     4201 tcatcttgag cttgtgatcc gggatcggat ccgacgggac gaccagcccg aacacggccg
     4261 agagcacgag caccgacgag tccgcccgcc ggcgggccgc agccgacagc gacggaaggt
     4321 cgagggcgtc gtagagcaca ccggtgaagc gctgcgcggc ggccatggtc ggggactcgg
     4381 cgacggtccg gttggcggcg gtcgccgcgg ccaacgcgtc gcccttcacc ccgagcagct
     4441 tctgacgttg cgcctcgttg cggcgcatgg ccgagcgcag ggccgccatc accgccaggc
     4501 gccggccgtc gaggtcgatc gccatcgtcg acggggccca ggcggggccg gaccccccgg
     4561 gcgcctttcc ctccgacggc gggatcagga tcagcggagg ctcgggcacg ggcgccgatg
     4621 atcgcgcgtc gagcgaccgc gaccagcacg gagcagaccg caggagcgcc cccggcagga
     4681 ttcgaacctg cgaccgtcgg attagaagtc cgctgctctg tccagctgag ctacgggggc
     4741 ggtggtggtg ggcggcaccg tatatgctcg cccctcttgt catggtggcc gtagctcagc
     4801 ccggttagag cgccaggttg tggccctgga ggtcgggggt tcaagtcccc tcggtcaccc
     4861 catgcggacg gcgccaccgg ttcggaccgg tggcgccggg tcgctacaat ccaactccgc
     4921 cgggtccgcc cggcggagtc acatgcgcct ctagctcaat tggcagagca tcggactctt
     4981 aatccgctgg ttccgggttc gagtcccggg gggcgtacac agcgaagggc ccggaaccgt
     5041 caggttccgg gcccttcgtc gcgaatccgg ctccgatcag gaccgggcgt cggttcaggc
     5101 ggcgttgaac ccgacgccgg gggccagggc gctgcggtcg acctcggcgc tgccgaagcc
     5161 gaggacgatg tgggagatgc ccgggatcag gaacaggaag atcttccagc cgagctcctt
     5221 gccgaacagc tccgcagtgt cgttggccag catccacatg gcgatgatgt tgatgcaggg
     5281 gatcagcgac aggatcagcc acaccaccgg ctcgtgctgg ccgcgggcct tgaacagcat
     5341 gtacacgttc acgagcggga tgatccccat ccagcccggc aggccgagct tggtgaagac
     5401 cttccagtaa ccgatgagga acaccacgaa cagggcgagg tagatgacca tcaggatccc
     5461 gaacgcggcg aagccgccgc cgctcgaggt ggtggtctcg aagtcctggg ccagggttgc
     5521 gagcatggtc cgaatctcct cccgaggtcc gcggggtgcg gatctccttc aggaacaccg
     5581 agcgtaagcg gccggccacg taaaacggcg cgatgcccgg tcgtcacaga gaacggccga
     5641 cgggcatccg accgcccgca gcggctccgc tgctcagcgg tgcagggacc ggtacaccgc
     5701 gtcgatcagc gcccggttgc gggtgctctg ccagcggggg atgacgtggt tcatcacgta
     5761 gccgaaggcg atcccgtcca cggggtccgc gaagccgagc gacccgccgg tgccgtagtg
     5821 gccgaacgcc ccggcgttcg ggcccaggcg acgccggtcc gtcgtcgtgg cgaaacccag
     5881 tccgtaggag agctcgtcgt cgaggatcgg gcagtgaccg accgcctgca ccgtcgtcgc
     5941 cgcccgcagc agcgcgggtg acaggaggcg atcgtcgtcg agcagcgccg agtagatgcg
     6001 ggccactccg gccgccgttc cgtgaccgct ggtcgatccc aacggcagcg accgccaccg
     6061 tggcgtgttc atcacaccca ccgacgagta gcccggcggg ttgaagtgcg ccagggcgtg
     6121 cagaaggttc aggccctcga ggcggtccag gtcgaacgcc gggatcctga acgcctcgtc
     6181 ccagaccgtg tcggcgcagc ggtgctgctg atcggacggc accccgaagt gcacgtcggc
     6241 ccccagcgga ccggcgagcg cggccagggc ctcgccgggg ccggcaccgc cgatgcgacg
     6301 gaccagctcg ccgaccaggt gaccgaaggt gttgacgtgg taggcgagct gctcccccgg
     6361 tggccaccac gcctcggtgg cggccagcgt cccggtcatc gtcgaccagt cgaacaggtc
     6421 gtcgtcgttc aggtgacgac ggatggccgg cacgccggcc cggtgggtga gggcatggcg
     6481 aacggtggcc gacgccttgc cgcccgccgc gaactcgggc cacaccgacg cgatcgggtc
     6541 gtcgaggccg acctctccgc ggtcgaccag tcgcagcagc agcagcgaca ggagcccctt
     6601 gcccaccgag tagaagtcgg tgatcgtgtc cgccgcccac ggccgggtcc gcgccgggtc
     6661 ggcccagccg ccgtggaggt cgacgaccac ctcgccccgg tggatgacgc agacggcggc
     6721 gccgagctcg ccctgttcga cgaagttgtc ggcgaacgcg acgcggaccg cgtcgaagcg
     6781 ctcggcgcag aacccgccga tcgggacacc gcccccgtcg tccatcggca ccgtcgcggt
     6841 caggccgctg tccgcagcac gcgctggttc caggggttga ccgagaggat gccgtgcagg
     6901 cggggcagcg tcgtgcgggc catcaggcgg gccccgcgca ccgcgctgag ctcggccggg
     6961 ttgagctcgc ccacgtcgag cagacgacgc atccggcggg gcagcagctc ggcggtcacc
     7021 cagttcagcg ggacgaacga ctggtcgggg atccagcgca gacccggacg catgaggacc
     7081 cgggtgatct cacgggactc gtcggtcggc tcgagggtgg cgaatcgctc ggccatgtag
     7141 ctccggaacg cggcgaggtc ttctgggatg agctcgtcgg ggatgccgaa cgcgcccgcc
     7201 atcagcttgg actcctcgaa gaaccgctcg cggtctgctc tgcgcatctc gccgacgtag
     7261 cgacgttcca cctcgaaggt ggtgtccacc agggtggcca gcacccagta ctgcagttcg
     7321 gggtcgttgg cgcggtactt ctcaccgtcg gctgtcgttc cccggacccg ggcgtggatc
     7381 gcccgcagtt gcttcagcat agcgtcgcgg cgttcgggag ccgcgaagct gatcgtcagc
     7441 atggcgtcga gggtgtggtc cagccgtccg atcgggttgg tgcggaactc gctgtagttg
     7501 gccacgccgg cagccacctt ggggtggcac acctgcagga tcgcagcccg gggtccgccg
     7561 atgagcggga accaccgccg gttgacgtcc caggagatcg tctcccgtcc gtagagcgcg
     7621 ccggactccg gagcaagtgc cgaactgacc atctggaacc ccccgtcccg tggactagcc
     7681 gtgatcggag ggtaccggct ccgggccacc cggtcgacga tccgccccct gatcgacgac
     7741 atccggtggt cagcgccgcg cgtcctcggt gaccgccacc tcgaacagca gcggccagta
     7801 cttgccggtc agaaggagat cgccggagtc gggcaggtgg gcgatgccgt tgagcacgtc
     7861 gatcggtcgg cccgcggcag cggtcgcagc ccgggccgtc aacgcggagg cgtcgagcac
     7921 gccgtcgacg cggcggcacc ggaggtcgat ccgcacgatc tcgtcggtct gccaccggtt
     7981 ggcccacagc cgctcaccgt cccactcgag ctcgttgagg cggtccgccg caccatcggc
     8041 ccgggtgatc tgccagcgct cgagcaccga gaagtcatcg gggtcgcgca cggtcagcgt
     8101 gtcggacccg tcgctcatga ccaggcgacc gtcgtcgagg gtgctgatcc cccacccctc
     8161 gccgtcgtag cggtgctcgc cgaccgcggt cagctccgac cggttccaca ccagggcccg
     8221 gccctcggtc cacgtgagct gcaccagacg gtcgtcgccg accgcggtca ggccctcgcc
     8281 gaacagctcc gggtccaggt cggtggaccg caggacccgg cccgagtcga ggtccacctc
     8341 gcgcacgctc gagtgcccgc gccggccggt cgactcgtac agcgcgccgt cgacgacgac
     8401 caggccctgg gtgaacgccg tcgggtcgtg cgggtgctcg gcgagcacct cggcccgcag
     8461 cggtgtcggc tcgggcgccg gtccgcagtc cgtggggatc gatcccgcac ccgatggcgt
     8521 gtcgtcggat ccgcacccgc cggcgagcac cgccacgacc gccagagcga gcagcggtgt
     8581 ggtcgctgcg cggcgcggcc acgagcgtcg cgtcacacga gctccagcgt ggagaccacc
     8641 tcgacgtgat gggtgtgggg gaacaggtcg aggacctcga tgcgggtcgc tcggtatccg
     8701 gaggccgtca gcatctccag gtcccgcacc atcgctgcgg cgtcgcagct gatcaggacg
     8761 acccgtcccg ctcccgtcgc cgcgacggcc cggacgccgt cccggccgag ccccgagcgg
     8821 gccgggtccg cgacgacgac gttgaccggc gacggacgcc aacgctcgac cgcgctgcgc
     8881 acgatggtgg cgtcgcggtc cgccaggttc acccgggcgt cggccaccga cgccgcggac
     8941 cgctcgacga cgatgggccg ctcagcgccc agcaccgcgg cgaagagtcc gacccctccg
     9001 tagaggtcgg ccaaccggtc cgacgaaccc agggtcccga ccgcccgacg caccgaatcg
     9061 accagcgcct cggccccgct gggcccggac tggaagaacg agcgggccga gatgcgcagc
     9121 ctgaccccgg cgacctcctc gtggaaccag gcccgctgcc ccgcggacag ctcgacgcct
     9181 ccgatcaccc ggacaccgtc ggggaccacc gcgtcctggg ccgcccccgt gtcgaggatc
     9241 accagacgct cgccggtgcg caggccggcc cggatcgtca cgtcgactcc gtccgggaac
     9301 cgtccatcgc ggatcacctc ggccaccggg tcggccgcca ccgcacaatc cgcgaccgcg
     9361 agaaggtcgt cgctgtggtg acggtggaag gcgaagcggc caccgtcgac gccgccacgc
     9421 accgtgctgc gataccccca tggatccgcg gccggaccga cctcgaccgg aacggcgtcg
     9481 aatcgcccgg cacggaccga tcgggccagg gcatcggaga cgatggcggc cttgagcgag
     9541 cgttgggcgg ccggggcggc gtgctgtagg tcgcatccgc cgcacccgcg ggcgagctcg
     9601 ggacagggcg gctccacccg gtccggcgag gcggcgatga cctccaccac ctcggcgtcg
     9661 accgaccggg gccgctcgcg gagcacccgg atgcggacgg tctcgccggg aagggccccg
     9721 cggaccaggg cgacccgccc ggagtcctcg tgaccgaggg cggcaccgcc gacgaccagg
     9781 cggtcgacgg agatgaccgg ttcggtcggc gtcggctccg gcggcggctc cggcggcgga
     9841 gtggggtcgg gtcccggatc catggccgca cgacagtacc ggtacgatcc gtcctgaccc
     9901 gcccccgttc gtcgactcga ggcccacagt gtccgcgccc cagatccaga tcgtcccatc
     9961 ggtgcttccc gccgacttcg cccggctcgg cgaggagtgc cagtcgctcg agaaggccgg
    10021 cgtcgaccgg atccagtggg acgtgatgga cggcgtgttc gtccccaacc tcaccttcgg
    10081 acccgacgtg atagcggcgt gtcgaccgct ggtcgacgtt cccttcgagg cgcacctgat
    10141 ggtggtcgag cccgatgcgc tgctcccccg ctacgtcgag gcgggctgcg aggtgctgat
    10201 cgtgcacgcc gaggcgtgcc ggcacctgca ccggaccctg gcgtcgatcc gggacctggg
    10261 ggcgtcaccc gcggtggcgc tcaacccggc caccccggtc gacacgctgc gacacgtcct
    10321 cgacctggtc gacgagatcc tggtcatgac ggtcaacccc ggcttcggcg gccaggcgta
    10381 catcgacacg atggagccga agatcgccga ggtgcgagcg atgatcgaga tgtcgggacg
    10441 cgacatcgac ctggaggtcg acggcggcat cgggccggcc acggtctcgg gggccgctgg
    10501 cgccggcgcc aacgtgctcg tggccggcag ctccctgttc cgcgaccccg agggccgtgc
    10561 gcacgcggtg agcgagatcc gctcgatcgc caccgccgcg ttcgcctgag tgctgctgac
    10621 cccgatgccg gtgcggtcgc gtcgtcggaa cctggcgggg gccgtgctgg cggtgaccgc
    10681 ggtcgtcgcg gtgtcgaccg gctgcggcga cggccgaccg gcgttctgcg acgatctggc
    10741 ccgggccgcc gacatggacg ccctgtccgc agcgttgcga gcccaggacc tcgatcgggc
    10801 gcgcacggcc gcccgggagt tctcggacct ggccgacggc gcgcccgagg atctgcggcc
    10861 ggacatgcag gacctggccg gggcggtgag cgagatcgtg gacctgctga gcgccgagcg
    10921 caacgcgatg cccggcgcgg gccacgaggg ccagggtgac gccgcagtcg tcgaacgcca
    10981 gcgggacgag ctgaacgagc ggctcgacga cctcgccgtc accagctccc gcgtcgagcg
    11041 atgggcgtcc cgggagtgcg gcctcgatct ggactgacgg agttccgaca ccggccattt
    11101 gcacccggat ccggcggttc gggagactcc tgccccgacg gcgccccggt gcggtcctcg
    11161 accccgtcgg gcgcggattc ctcacatcgg tcgggtatcg accgatgggg tctcgtcatg
    11221 tggtagccat gtgttcgtca tggaaccgtc atatgctgac ggtttcgttc acatctacgg
    11281 ctggggccgt cgacagcacc ccacgcagcg accgacgaga aggagcattc ccccatggac
    11341 cgcccgtccc tgaactccct gtacgaagac cgcgattcgt ccgccgagca ggtaacgacc
    11401 acgacagccg cacgtcgcgg cggcgtccgc cgagccggtg tcgccgtcat gctggccggc
    11461 gtgctggcca tgttggtgct ggtcggttcg tcatgcaccc gcaacgcgta cgcgttcgac
    11521 tccgccgcac aggtgaacgc cacccggtcg agctcggggc tgtccaagct ggccatcgac
    11581 gacaccctgg tcgccaaggc ccaggcgtgg gccgagcgga tggcatccgc gggcaagatc
    11641 tcgcactcgc agctcaccga cgccgccggc aacaactggt cggtgctggc cgagaacgtc
    11701 ggcatggcct cctccatcag ccagatgcac tcgctgttca tgaacagccc gtcgcaccgg
    11761 gccaacatcg tcaacggcag cttcgaccgt atcggcaccg gcgtcgccga gtccggcggc
    11821 cgactgttcg tggtccaggt cttcgccggc tgatcgaccc cgatccgtta cgcgatgagg
    11881 ggccggcccg gcgggtcggc ccctcatcgc gttccggtgc gtcaggtgac gtcctcaggt
    11941 gacgttcagc gcatctcgcc ccgacgcacg atcacgtggt cgatgatgcc gtactccttg
    12001 gcgccctcgg cggtgaacca gcggtcacgg tcggagtcct cgttgatctg gtccttggtc
    12061 tgcccggtgt ggaacgcgat gcgctcggcc agcaggtcct tgatgtactt gagctgctcg
    12121 gcctggatgg cgatgtcgga cgcctggccc tgcatgcccg ccgagggctg gtgcatcatg
    12181 atccgggcgt ggggcagggc gtagcgcttg cccggcgcgc cggcgcacag caggaactgc
    12241 cccatcgacg cgctcaggcc catgcagatg gtgcccacgt cgggcgtgat gaactgcatc
    12301 gtgtcgtaga tcgccatgcc ggcggtcacc gagccgcccg gcgagttgat gtagagccag
    12361 atgtccttct cggcgtcctc gccctcgagg aacaggagct gagcggtgat gaagttggcg
    12421 accgtgtcgt tcacctcgga gccgaggaag acgatccggt tcttgaggag ccggttgtag
    12481 atgtcggcgg agggctccat gccgttgccg gcctgcatca ggacggggtc cgcggcgatc
    12541 tgcgggggga ggtcggagga tccgagtggc tgcatggcgt tggaggctaa cgcggtgcac
    12601 gcccggctcc gcacgcaccg gtgcgggcga ccgggcccgg tcgcccgacc ggtacggtgc
    12661 gtgcgtcacg cggacgtggc ggaactggca gacgcgctgg gtttaggtcc cagtgcccga
    12721 aagggtgtgg gggttcgagt ccccccgtcc gcactcaccg gtagcctcgg acggtggccg
    12781 ctaccgccgt cctgctcctg tcctgccccg accgcaccgg cgtcgtcgcc gcggtcgccg
    12841 acttcgtgtg gcgccacggc ggcaacatcg tcgacgccga gcagcacacc gacgacgtcg
    12901 acggcgtgtt cttccagcgg gtcgagttcg agctcgacgg cttcgccttc gagcgcgacg
    12961 acctgcccaa ggcgttctgg gccgtggcgg agcggttcga catggacgtc agggtccact
    13021 tcagcgacga acgaccccgc ctggccatcc tggcctcgaa ggcgtcgcac tgcctggagg
    13081 acctgctcaa ccggtggcgg gcgggtgagc tgcccgccga ggtcgccctg gtggcctcga
    13141 accaccctga ccaccgggcg ctggcggagt tctcgggggt gccgttccac cacctgccgg
    13201 tcggcgacgg acccgacggt gcagcgcgtc aggaggcggc gatgatcgag ctgctcgaac
    13261 gggaacaggt cgagagggtc gtcctcgccc gctacatgcg gatcctgtcg ccgacgttcg
    13321 tcgagcggtt cccgtcgcgc atcatcaaca tccaccactc gttcctgccc gccttcgtcg
    13381 gcgcccagcc ctaccgccag gctcatgagc gcggcgtcaa ggtcatcggg gcgaccgccc
    13441 actacgtgac cgcggagctc gacgagggcc cgatcatcga ccaggacgtc gcccgggtca
    13501 gccaccgcga cagcgtggcg gagctcgccc gcaagggccg cgacctggag gtcgccgtgc
    13561 tggcccgtgc cgtgcgggca gatctggagc accgggtgct gccgttcggc aaccgaaccg
    13621 tcgtgttcca ctgagtccgc tgtcctgagc agcggacccc ttcgcggagt tcggccagac
    13681 tgctgcggtg cccgagggac acatcatcca ccgactggcc cgggagatcc gctcgacgct
    13741 gggcggagcc ccggtcctcg ccagttcccc ccagggccgc ttcgccgacg gcgccgccgc
    13801 gatcgaccga caccgactgg tgcgcaccga cgcgtggggc aagtacctgt tctgcgactt
    13861 cgggaccggc gagatcctgc acgtccacct gggcctgatc ggcaagttcc ggcggcgggc
    13921 gacacctccg ccggatcccg tcggcgtcgt ccgcctgcga ctcgtcggac agggcacgac
    13981 gtgggacctg tcgggcccga cgcgctgcga gctgatcacg cccgaggagc aggaccgcat
    14041 cgtcgccggg ctcggcgccg acccgctccg tcggggtgcc gacgtcgcca cgatgcgcga
    14101 ccgcttcgcc cggtcgggcc gtccggtcgg cgcggtgctg ctcgaccagt cgatcgtcgc
    14161 cggcatcggc aacgtctacc gggccgagct gctgttcctg tgcggcatcc gaccggaccg
    14221 tgccgccgcc tccctcaccg acgacgagtt cgacgagctg tggcgacgga ccgtcgagct
    14281 gatgcgggtc ggcacacgcc tcgggcgcat cgtgacgacc gaacccgacg agatcggacg
    14341 gtcccgaggg cgcatgggcg acgaggaccg cctgtacgtg taccaccggg agcactgccg
    14401 acggtgcggc ggaccgatcg cgaccgcgga gatcggcggg aggcagatct ggtactgccc
    14461 cacctgccag ccggccaccg cctgaccgcc ggctatcccc gatccggccg tccggaccca
    14521 gccgtccggg acgcaccggt cccggaccag gagcgggtac cgtccggccg gtgaaggtcc
    14581 gcgacctgcc cacaccgtcg ctcgtcgtcg acgtcgacgc gctcgacgcc aacatcgcca
    14641 ccatggccgc cgcccggccg ggtcgcactc tccggccgca cgtgaaggcg ttcaagtcga
    14701 cggagttggc gcgccgggtg gccgagctcg ggggccacca ggccttctgc tgcgcgaccc
    14761 tgcgcgagat ggagggcatg gccgacgccg ggttgggcga cgacctgctg ctggccaacg
    14821 agacgctcga cgccccacgc ctgcgccacc tggtcgaccg cggtgacgcc cgcgtgaccg
    14881 tggcggtcga ctcccccgag acggtcgagg tggccgcaca cgccggggcc gaggtgctcg
    14941 tcgacgtcga cgtcggactc ccccgctgcg ggtgccggcc cgaggacgcc ggtcgtctgg
    15001 ccgagctcgc acggacccgg gggctgaacg tccgcggcgt catgggctac gagggccacg
    15061 tcgtcggcga ccccgaccgg ggccgtcggg ccgagaaggt ggccgagtcg atggccttgc
    15121 tgcgccgcgc ccacgacgac gtcggcggcg acgtcacctc ggcgggcggg accggcacct
    15181 acgacctgca cgactgggcc ggcgaggtcc aggccgggtc gtacctgctg atggacaccc
    15241 actacgccgc gctcggcctt cccttccgac aggcgctgcg cctcgaggtg acggtgatct
    15301 cgacctcgcc ggaacgaggc tggggcgtgg tcgactgcgg gctcaagtcg ctgggaatgg
    15361 accacggcaa cccgacgatc tcgggctgga acgtgttctt ctgctccgac gagcacacca
    15421 cgttcctggc cgacgccacc ggggactccg agggtcgggc cctccccgcg gtcggcgccc
    15481 gcctgggggt gctccccgct cacgtcgacc cgaccgtcgc ctaccacgac cgcctgcacc
    15541 tcgtgcgcgg cgacgaggtc atcgacgtgt ggccggtcga cctgcggggc tggtgagaag
    15601 atctgcgcag ctcagggcag gcgggcgagc tgcggggcga tggcccgcaa cgcccggccc
    15661 cggtggctga tggatgcctt ctcgatcccc gacatctcgg cgaaggtccg ccccggggcc
    15721 gaggcatcgc cgaccgggtc gtcgggtacg aacaccgggt cgtagccgaa gccgccgttc
    15781 ccccgcgggg ccccggcgat gtggccctcg acggtgccgt cggccaccag ttcgatcacc
    15841 gagccgtcgg cgtccggcgg tgactgcacc acgatgcagc agcggaaacg ggcccgccgc
    15901 tcggccgcgg tgaccgcgcc gaggccgacc agacggtcga gcagcagtgc gacgttgtcg
    15961 gcgtcggagg cctcgtcgcc ggcgaatcgg gccgaccgga ccccgggcgc gccgtcgagg
    16021 gcgtcgacct cgaggcccga gtcatcggcc agcgcccact cgccggtggc cgcagcgacc
    16081 gcggccgcct tgatccgtgc gttcccgatg aagtcgggag cgtcctcggc cggaacggcc
    16141 agatcggcgg gtcgggcgac gacgtcgagg ccgatgccga gcgagaccag caggtcctgc
    16201 agctcggccg ccttgtgggc gttggccgac gcgacgacca ggcggcccgt caccggctca
    16261 gcgaccgatg ggtcggggtg tgggagcctc ggccaggtac ttgcgctgaa gggcgtggat
    16321 gcgacggatg ccgccttcgg ccaggccgag cagcgagtcg agctcgtcac ggctgaacgg
    16381 cgcgccctcg gcggtgccct ggacctcgac gaacaggccc ttgccggtca tgacgacgtt
    16441 catgtcggtc tcggcggtcg agtcctctgc gtacgggagg tcgagcaccg ggacgccgtc
    16501 caccacaccg acggagatcg cggccagctc gtcgtagagc ggcacctcgg acagcaggcc
    16561 ggcctgcacc acccgggtga ggcagtcgtg cagcgccagg taggcgccgc tgatcgacgc
    16621 ggtgcgggtg ccgccgtcgg cctgcagcac gtcgcagtcg atgaggatct gacgctcgcc
    16681 gagggccttc atgtcgcaca ccgagcgcag cgcgcggccg atcaggcgct ggatctcctg
    16741 ggtgcggccc ttggggccct tggtctctcg gcggatccgc tcgggcgacg agcccggcag
    16801 catcgagtac tcggcggtga cccacccctt gccggtgttg cgcatccagc gggggacgtc
    16861 gtcgtcgacc atcgcggtgc acagcacctt ggtgtcgccg caggagacca gcaccgagcc
    16921 ctgggcgtac gcggtgtagt cacgctcgaa ggtgatgggc cgcagctcgt cgggggcacg
    16981 tccgtcgggt ctcactggtt gctcctggca ggcggggccc gggccggagg ctcaggccag
    17041 gtaggtgtgg tggatccggg ccggatcgac cggagcacca taggcggcgg acgcctcggc
    17101 caccgccagc tcgaccgacc cggtgggcgg gacgtgtgtg atgagcagct gggacacacc
    17161 gtcggcccgg gccagccggc cggcctgctc cgcggtcatg tgcaggtcgt cgacgtcgaa
    17221 gccgtcggcg gccagccacg tcgcctcgca gatgcccagg tcgatgccgt cgcccagacg
    17281 gccgagcgac cacgccgggc cggtgtcggc cgagtacgcg atggagcggg tggacgctcc
    17341 atcacccagg tcgacatcgc ccatgtcgac gcggatcccc atcgtctcgg gcgggtgcgc
    17401 cgtgcgcgag gtgcgcacgc gcagggcccc gacggtgacg tcggccccgt cggtgatgac
    17461 cgacggggcg aaggcatcac cgaggccgtc gtggaacacg ccgtcgagga tggcgagcgt
    17521 gtcggcggtg gtgaagagag ggacgccgtg gtggccgagc acgtagcgca gggcgttgcg
    17581 catgaccggc acgtcgcccc agtggtcggg atgcgagtgg ctgatgacaa ccgcatcgag
    17641 ctcggtcggc tccagatggt gctggaggct ggagagcacc cccgggccgc agtcgagcag
    17701 cactgcggtc ccgccgcctc ggacgaggta gcccgagcac gcgttgccgg cgccggcgaa
    17761 ggtgccgccg cagccgagca cggtgaccgc cagccgtgac ggatccggga ccacgtcggt
    17821 ccccaggtcg gtcaccatcg ctgcgtccac gacagcagcc tcacgatgac agcaccctca
    17881 ccacgacagc ttctcggccc ggtcgagctc gggtccgagc aggcgacgac cgagctcctc
    17941 gaaccagttg acgtcaccgg tggtgatgaa ctcggcgcgc ccgcacgagg cgcgcccgga
    18001 cgcgtcggcg cccgtggcgg gccgggcgag cccgagatcc gagaggaccc ggcgcacctc
    18061 gaaggccgtc tcgtcggccg aggagaccag caccacgtcg cgtcccatca cgtcgttgag
    18121 ggtacgggcc aggtacgggt agtgggtgca accgagcagc agcgtgtcga ccttggcctc
    18181 cctggccggc tcgagcagcg cccgggccag cacgtggacc tgctcggagt cgaactcgcc
    18241 ccgttcgacg aactcgacga agcccggaca cgcgagcgcg atcagctcgg cgtcgtccga
    18301 cgcggcggcg acggcacgct gataggcacc ggagttgatc gtcccgacgg tgcccacgac
    18361 gcccacccgg ccggtcacgg tggcctggac cagggcggtg acgcccggtt cgatcacccc
    18421 gatgaccggc acctcgaagc ggtcccgcag cgagtcgagc gcggcggccg acgcggtgtt
    18481 gcaggccacg acgagcagct tgaccccgcg acgctcgacc agctcacggg cgatctggtg
    18541 ggcgaaccgg gcgacctcgt cgaggggacg ggggccgtag gggtagcgac cggtgtcgcc
    18601 caggtagacg aggtcctcgg ccggcagcag gtcgatgacg gcccgggcga cggtgaggcc
    18661 gccgaagccc gagtcgaaca tgccgatggg accgtcacgc acgggctcca cggtaccggc
    18721 gcccggacgt cagatcgggg tcgacggcgg ccgagcgatc agaagtagat gaccttctcg
    18781 gcgttggcgg cggcctcgtc gaggtccagg gtccacgccc cggtcgacag gtacttccag
    18841 ccgtagtcgc tgacgatgaa gacgatgaca ccggactcga tccgcgaggc gaccttgacc
    18901 gccccggcca gagcggcccc cgcggagatg ccggagaaga tcccgacctc ggccatgcgg
    18961 cgggtgtact cgagcgactc gcggggccgc acgatcgact tgccgtcgag caggtcgtag
    19021 ccgccccact tgtcgaacac cggtgggatg tagccgtcgt cgagggaccg gaggccttcg
    19081 accagctcgc ccgacggcgg ttcgacggcg aacacctgga cgtcggggtt ctgctccttg
    19141 aggaaggtgc cgcagccgag cagtgttccc gaggtgccga gtccggcgac gaagtgtgtg
    19201 atgtcaggga cgtcggacca gatctcgggg ccggtcgtcc ggtagtgcgc ctgggggttg
    19261 gcctcgttgc cgtactggta caggaacacc cagtccggat gctcctcgga cagggcctgc
    19321 gcccggcgca ccgcgccgtt ggagccctcc tcgcccggcg aggggatgat ctcggcgccg
    19381 tagacctcga gcagttggcg tcgttcgatc gacacgttct cgggcagcac gatcttgagg
    19441 tggtagcccc ggatgcggct gatcatcgcc aacgcgatgc cggtgttgcc cgacgacggc
    19501 tcgaggatgg tcgcgcccgg cgacagggtc ccgtcagcct cggtgtcgag gatcatcgcc
    19561 agggcgatgc ggtccttgac cgacccgccc gggttctgac cctccatctt ggccaggatg
    19621 cggacgcgcg ggttgggact gagctggctg acgtcgacga tcggcgtgtt gccgatcagg
    19681 tcgaggacgg acgagtagac ggtcattctg gaggcctccg aaacctgacc cacatggtag
    19741 ggattcaggg gtcccgccga ccactcatcc gggtggtcgc ccgtcaccga accagatcga
    19801 cgcgctcctc ggcgatcgtc ccgtcgacga tgcggtacga ccgcagggtc ggctcgtcgt
    19861 cgcgcaacga cacgatcacg tagtgccacg aggggtccgg agcggatgcg acatcggtcg
    19921 gcgagggata ggcgtcggtg tgggtgtggc tgtgcatcac cccgacgatc gccagtccgt
    19981 cgtcctccgc agcgcggtcg gcccgcagat ggtcgaccgg gtgcacggtg tagacgacgg
    20041 cgctctcggc cgcgttgcgg cacggcacga agcggatcac ccgctgctgc gggtcgtccg
    20101 ggtcgccgac gagcaggccg caggcctcga gcggcagccc ccgcagggcg tgagcggcca
    20161 tctcgtcgtg gagctcgggg gtgatcgcca gcacgggcgt caagcgtagg tggtccggtt
    20221 cacgacggga tcccgtggag gccgatcggc tccgggcggt accgtgcagc acccatgaag
    20281 ccgaccggta cccagatcgt cgccaccaac cgccaggcgc gacgcaacta cacgctgggc
    20341 gacaccttcg aggccggcct ggtgctcaag ggcagcgagg tgaagtcgct gcgggagtcg
    20401 aaggtgcagc tcaccgacgc gtgggcctcg gtcgacggcg acgaggcctg gctcaacggc
    20461 cttcacatcg cgccgtacac gctggcgcag gcccactcgg gccacgagcc ggtccggcgc
    20521 cgcaagctgc tcctgcaccg gggtgagatc gaccggatcc gcatgcgcat gcaggtcgag
    20581 cggctcgctc tggtgccgct gtcgctgtac ttcaaggacg ggcgcgtcaa ggtcgagctc
    20641 gctctgggca agggcaagaa cgtggtcgac aagcgccagg acatcgccaa gcgcgacgct
    20701 gaccgtgaag ccgcccggga gatctcccgg gcccgtcggg gcgactgacc gtgatccgca
    20761 gtcgtcggcg caccgtcacg gccgccgtgg ccgccgctgt ggccgctgta gcgctagccg
    20821 ccgcggcgat cgtgatcgcc gtcgcggtgg gctcggacca cacgacggtg gcgaccggtg
    20881 cccgagcgtc acaacgctca gcaacccggc ccaccaccgc tcccaccacg acgctggccc
    20941 cgagcacgat tccgccgacc accgcaccgc ccagcacgct gccacccagc acggttccgc
    21001 cgaccaccgc acctgccagc accgcacacc cgagcactgc agtactcagc accgcacaac
    21061 cggcgacggg tgcacccgcg ccggcgccgg cagctccgcc agcgccccct cctgctcgga
    21121 ccaagggccc gatctcggtg ctggtcaccg gcgactccgc cgggttcgcc gcgtccttcc
    21181 ctgccccggg cccacgggac cgtccgccgt acatcacccg gatcgacccc gcggcagtga
    21241 tcggctgcgg ggtgctgcac cgacccggct tcaccccggt cgacctcgcc gacgagggcg
    21301 ccggtggctt cggtgggtgc gcgacgcagt accgcagcga gctggccggc ctggcccgtc
    21361 ggccgaactg gatggtgatg ttctccggcg gttgggagca cctgccctgg atccctcccg
    21421 gcgaccgagc cgctccccgc tctctcacct gagctccgtc gccagatcgt caacgagctg
    21481 gtccgtcggg ccgatggggc gatgagcatc gggacgagaa ccgcgttcgt gtcctgggca
    21541 tgcccgtcgg gggtcgcccc cacccggacc ggggactacg cccgctggta cgaccagatc
    21601 ctgcgcgaag ccgcctcccg tgctcgcggc gccatcgtca tcgagcctac cgaccgggtg
    21661 tgcgtgggcg gcgaccccgg cgcggccgcc actccggaga aggactgggc cttcgccgga
    21721 acccagcacc cgcacgaccg tcgctggctc tggaacgagt ggctgggccc gatcctgctc
    21781 gccaacagct gagcacccgg ccccggttgg tgccgggtgt ggtcgggccg gtacccgaac
    21841 cgagttcgca tccggctggg ccgttcggcc catttcgccc gacgcgctcc agtccgaacg
    21901 ccccaacgtc cgaagggtgt cacgggtcga cgcccgtcga ccccggttcc caccgacgca
    21961 gcaccagaca gaaggacggc accaatggtc cagtacgagt tcctcaaggc ctggctctcc
    22021 gcacaggccc gcaccgaacg aggcgcatcc ctcgtggagt acgcactcct cgtggccctc
    22081 atcgcggtgg tgtgcatcgc cgccatctcc ttcatcggca cctcagccag caacaagttc
    22141 agccagatcg gcagctcgat ccagtagtcg ctcgctctcc agcagcgggc aacccgtcgg
    22201 ccggtgacca cgtcggtcac cggccgatgc gcgtggcggg gaggcacggg atcacagccg
    22261 ccggaacctg ggcgaccggt gtcggcccgg accggtacga tgacggacac acacggggct
    22321 gactggtttc gacgttggga cctggaagtc gagcgtgcga cccgagttgt ctcggactcg
    22381 ttaaacaggg acacacaaag aaccgccgac aacgagcagt tcgctctcgc cgcctgatcc
    22441 ttcaggtgac gtagaggaaa atcgtgacca gcgatggcgc acggggccgg acccccacag
    22501 cccggcaaca gaagtggggg tggaccgcag ggagagaccg ctgacggcgt gaaagaccgc
    22561 tgacgccggg gaaagacccg gcagtgggcc cttcggggcc ggccgacccg ccggttcagc
    22621 tgctgtgagc tgagaagtcg ggaatggtcg tagcaggttc ggtgaccacc tggcggacgg
    22681 gggttcgatt ccccccagct ccacga
//
LOCUS       DAOQOD010000030        22130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_801_length_22130_cov_12.871302, whole genome shotgun sequence.
ACCESSION   DAOQOD010000030 DAOQOD010000000
VERSION     DAOQOD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 22130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22130
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_22130_cov_12.871302"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLZ72_04900"
     CDS             complement(<1..771)
                     /locus_tag="PLZ72_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA35668.1"
                     /translation="MSTAPSSSARTTDGHGPNDLDVVKVRADFPILSTEVHDRPLVFL
                     DSAASSQKPAAVIEAMDRYYETTHANVHRAAYSLAEAATNAMEGARARVARFIGATSP
                     DEVVFTKNATESLNLVARSWGDANLGPGDAVVLTELEHHANIVPWQQLAAERGTELRW
                     IPLTDDGRLDLTDLDRLLDGAKLLALSAMSNVVGTITPVRMLTDAAHAAGALACIDAC
                     QYVPHVATDVDALGADFLAFSGHKMLGPTGIGVLWGRPE"
     gene            complement(768..1541)
                     /gene="sufC"
                     /locus_tag="PLZ72_04905"
     CDS             complement(768..1541)
                     /gene="sufC"
                     /locus_tag="PLZ72_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNA35669.1"
                     /translation="MAVAPLLRIEDLHAAPAENLEAPILHGVSLTIHPGEVHALMGPN
                     GSGKSTLASTLMGSPEYSVTGGSLHLRGDDVTDWTPDQRGRHGMFLAFQYPQEIPGVS
                     VRQFLRQALSERKGIDLSVLEIRVAIMEWMKRLDMDPSFADRFLNEGFSGGEKKRNEV
                     LQMAILEPDVAILDETDSGLDIDALRVVADGVDEVRKDRPELGVLVITHYQRLLEHLT
                     PDVVHVLVDGRIVESGGPELARRLESEGFDAYREPEAVA"
     gene            1655..>1994
                     /locus_tag="PLZ72_04910"
     CDS             1655..>1994
                     /locus_tag="PLZ72_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HNA35670.1"
                     /translation="MPVTFLAHQAPVLPLKRRWPGLDGVALVVGSTVPDLARATERLP
                     VLYLYGTPTWFDGHRPDQLVSWCLVVGLLITWSARRLVLPRLGGHLPDLGDFHLRDLR
                     LVGRTRHRWWN"
     assembly_gap    1995..2094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2161..2784)
                     /locus_tag="PLZ72_04915"
     CDS             complement(2161..2784)
                     /locus_tag="PLZ72_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA35671.1"
                     /translation="MTDGTGTLHVSDTDMEIHKVVVGPMDNNVFVLRCRHRGDAVLID
                     AANEHDVLLDLASSLGVRRVIETHGHWDHIQAIPEMRDAGYSVGVTADDAGMLPSYDE
                     LIGDDEVIEVGDLRLRTVLTPGHTPGSICFRVEGKPILFSGDTLFPGGPGATQFEGGD
                     FPTIITSIEDRLFSRLAPDTLVLPGHGDDTTIGAESPHLQEWIDRGF"
     gene            complement(2835..3242)
                     /locus_tag="PLZ72_04920"
     CDS             complement(2835..3242)
                     /locus_tag="PLZ72_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35672.1"
                     /translation="MTVALDTTGLLSRYLDGPTRPIALAAMAADPDWCASALALSEAL
                     MLVDRLGDDPSRADELRRALRDDWERINVVPVDQLCLDRAAELGRTQPLRTVDAIHLA
                     AADRLPRPVTFVTFDPTQIPVALALGFDVVGSR"
     gene            complement(3256..3534)
                     /locus_tag="PLZ72_04925"
     CDS             complement(3256..3534)
                     /locus_tag="PLZ72_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNA35673.1"
                     /translation="MDQIGIRELRADVATQVRRAGDGHRVIVTVGGRPVAQLGPIEAA
                     DRQTSLADLAARGQVLLARRQDRPDPSTVLPLWAGTRIDQLVREVRGR"
     gene            3960..4700
                     /locus_tag="PLZ72_04930"
     CDS             3960..4700
                     /locus_tag="PLZ72_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35674.1"
                     /translation="MSAMWRYKGMCVVIVVVCVLASTAIGFLLTPEPSATATIALKTP
                     SQDNVLAPGSTGDASLARYTAQRARFVTSDAVLGNAGANLGVDDLNELRDQLEVEPST
                     DSNIVTITASGTTSDDAVRLATEVTRAYGEETEKQVNDLTDAALRSIDESAGQVRATI
                     TGSNAAVNEAAASTLGQLQQKAATIRTSSAVLGDGVEFVVNPDESSVSSQQLPIREAA
                     LGLILGLVIAGTVAYLRADSDAAKQRRA"
     gene            4756..5571
                     /locus_tag="PLZ72_04935"
     CDS             4756..5571
                     /locus_tag="PLZ72_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNA35675.1"
                     /translation="MTSGAPRPAGVRPAEDESYGDTVRRLAAAQKSGRGAPAYSRFVN
                     RKLGRLFAAAGFHLGLTPNGITFISAAFSFSGLVLLAAMRPSWWLGFAVTALLVVGYA
                     LDAADGQLARLRGGGSVTGEWLDHMVDCLKISTLHMCVLISWYRFLVTDPRTADPIDE
                     RWLLVPMGYAVVAAVSFFAQILNEQLVRNAGLRSASPTSAASADAGTPSILVSLLKVP
                     TDYGLLCLIFLLLGASELFVGAYTLMFVGSAAYLMAASVKWFGQMKGLDRGAS"
     gene            5568..6197
                     /locus_tag="PLZ72_04940"
     CDS             5568..6197
                     /locus_tag="PLZ72_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35676.1"
                     /translation="MTVSPHHDRLRKGRLARRVGSSAVVALSILVPYGCSSSDDSNPT
                     RSTEAAVTTEPGDGTVVGPTTTGVPATAPDTAPVADIVKEPVAPGTPAPLGAGVSVVL
                     VDSENLDVEARVPGETAGPAVAATLEVRNDSSEPFDLSSLAVTATYGDGTPAIGNDSE
                     PARALTGTVKAGASAKGVYVFRAPVKAADTVIVEVQSADRPNVVRFRVV"
     gene            6368..>9064
                     /locus_tag="PLZ72_04945"
     CDS             6368..>9064
                     /locus_tag="PLZ72_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA35677.1"
                     /translation="MDTKSSCSTSGAFRSRALRAVAAVSAALMAVSVLVVAPASADSA
                     PTAPGTPTTVTADSLPTVQINGVVWDQVVVGNTVYATGSFTQARPAGAAAGTNETARS
                     NILAYNITTGNLITTWAPSLNAQGRAITASADGSTIYVAGDFTSVSGTTRNRVAALNA
                     TTGAVISGFNANVNAHVNDLEVSGSTLYLVGAFTVVGNQARSRLASVSASSGAVTAWA
                     PSSNGEVRTLTVPAGTGKVVVGGHFETLNGSTQTGMGALDASSGSVLSWPANTVVKNH
                     GTEAAIWSLKSYGSVVFGNGMSFASTGNFEATFSSDTNGNLIWVNGCRGDNYDNVAVG
                     TVLYTVGHTHDCSSAGGLPQTNPDWTFQRALAMTTYADPSGAVNTSKWFTGRPVSQLL
                     HWLPTVAAGTFTGQGQGAWTIEGNSQYVAMGGEFPRVNGVNQQGLTRFAVTGLAPNKE
                     GPQDYDHLTPKLVGIAPGSVRVSWTTTWDRDNERLTYEVLRGTTVIATTEADSNWWTR
                     PEMAFIDRGATPGSTQSYRVRVRDVFNNILPSASASITVPSGTPSVGAYQAAVAADGA
                     NRYWRLGEGSGSTGWDSANGNDLTLSGVTRNVGGAISGDADTASTFDGSATVPGTTTG
                     WISAPQTFTIEAWFKTTTNRGGKIIGFGSSSSGSSATNDRSVYMDNSGRIRFGATSAS
                     ALTSGTGYNNGQWHHVVASMGPSGIRLVLDGNQVGANSSIKTAGYYWGYWRVGGDQLS
                     GWPSRPTSNSFAGTLDEVAVYPTELTVAQAANHNALGRGAAGNVAPTASFTASASGLV
                     ASVDGSASSDADGSVVSYAWNWGDGSAAGSGVSATHTYAAAGTYTITLTVTDDDGATG
                     TTTRSVTVTAPPSNVAPVASFSASASGLVASVNGSASSDADGS"
     assembly_gap    9065..9164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9530..15652
                     /locus_tag="PLZ72_04950"
     CDS             9530..15652
                     /locus_tag="PLZ72_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA35678.1"
                     /translation="MASSIAAVALGVGVAGADTAPVDPTEPETVAADSLPTVQIDGVV
                     WDQVVVGNTVYATGSFTSARPAGSAAGTNETPRSNILAYDITTGNLITSWAPTLNAQG
                     RTLEASADGNTIYVAGDFTQVNGTTRNRVAALDATTGVLRPFNPNVNAHVNSLALSGD
                     TLYVGGAFTVVGNQGRSRLAAVDAASGALREWAPVANLEVRAVVVPANKGKVVVGGHF
                     DDMNGVPTRGLAALDPTTGALVPFPANQIIQNWGADAAIWSLSTNDDQVFGTGFTYLV
                     NNDPTTGGNLESTFAADATTGELMWVSGCRGDHYDTAPIGNVLYNVSHAHDCAPVGGH
                     PQTSPWTFQRAQAETIYPDPSGAVNVGGKFNGRPRARQLHWLPTMAIGTFTGQAQAAW
                     TVEGNSQYVVMGGEFPRVNGANQQGLARFAVKSIAPNKDLLQGYPEFTPTLVPISAGS
                     LRISWQGVWDRDNAQLTYEVLRGATVNTSTVVATRTYSPSNWWYRPMLAAVDASAAPG
                     STETYRVRVRDAFGNGFVSPAVTGTVPAGTPTPSPYRDAVIADSPAKYWRLGEAAGTI
                     GYDQVGADDLTLNSNTTRGVVGALIGDSDTSTTFAGGTAPASTNGTAVNGPQQFTVEA
                     WFKTTTSSGGKIVGFGKSSTGSSTSYDRHIYMTNGGQLRFGVYDGSTRTVASSAAYND
                     GQWHQAVGQLGPAGIKLYVDGKLVGADASATSAEAFAGYWRIGGDALGGWPSQPSSAN
                     FNGTIDDVSIYGGELTQDQIRAHFLASGRTPGWPTVPADAYGAAVWNANADLYLRLNE
                     TSGSTAVNRMTLDPGATASGTVWGGAASPADPSGTSVEFNSNNDRVVGTQQYTNPTTF
                     SLETWIRSTDTNGGRIIGFGDSTGQTSSNYDRHLYLLADGRLRYGVWVGSAATIDSPQ
                     AVNDGQWHHVVVTQQPGEQKMYVDGVAVASGTASAQQNYSGYWRLGADNIWSGDRHYR
                     GLIDEAAVYGSVLSPEAVLSHWTAAGGLAPNVNPTASFTAGAVDLAVTFDASGSSDPD
                     GTIVSYDWNFGDGATGSGINSAHTYGAAGTYNVTLTVTDDRGGSATATSQVTVLAPNV
                     TPTAAFSAVVDHLDVDFDASISNDPDGNVVAYAWDFGDGTPTVEGIAPTHTYAGAGTY
                     NVTLTVTDDRGGVGTVTLPVTATLPPNVLPTAAFSSGHVDLTATFDASASSDPDGTIV
                     SHAWDFGDGTQGNGATTAHTYLAAGTYDVTLTVVDDRGGQDTVTHEVTVTDPPNLAPT
                     ASFTFGHTDLTANFDASLSSDPEGRPLSYAWTFGDGSGTSTSVAPTYTYAAAGTYGVT
                     LTVTDDAGATASVTWPVTVTDPPPANVSPTASFSSNTSFLTADVDGRGSSDPDGSIVS
                     WSWNWGDGTAPGSGATASHVFAGAGTYTVTLTVTDDDGAIGTVTGQVTVSAPPANVAP
                     TASFTASANDLTLSVDASTSTDPDGSIVSWSWVWGDNTAAGAGSAATHTYAGAGTYTV
                     TLTVTDDDGATDTTSRTVTVTAPVVSNYASDTFSRTVSNALGTADVGGAWTTTGSAAS
                     YSVSGGLGRVSTTAGASRTITLGSVAATDTDLRARLGFDKTQTGGGSYVGIIGRRVNS
                     TNDYRLKLRFQSNGSVTAQLVRIVNGTETVVQNLSTVPGLTVNGGEFVRVRLQVTGTN
                     PTNLAAKVWKDGTAEPTAWQLQATDASASLQANGSVGIWDYLSGSATNGPVTMLIDDV
                     VAGPVGGGTPPPTNAAPSAALTSSVTGLDVAVDGSGSSDGDGTVESYSWNWGDGTPAG
                     SGVTAAHTYAVAGTYTITLTVTDDDGATGTSSSTVVVTSGPAPTNFVEDSFGRDTTNG
                     WGSAEIGGAWSTTSTASAYSVSGGAGRITMNAGAGRYASLNSVTQTASDVRASLSLDK
                     AQTGGGTYMALVGRRVDGSNDYRVKLRVQAGGAVTAQLVRVVNGTETVIQSVATVPGL
                     TWNAGEVLHVRFQVSGTGTTNLAAKVWKDGTAEPTGWTLSGADQTAALQSAGGVGIWS
                     YLSGSATNAPMTLTVDDLVAGPLG"
     gene            15786..17204
                     /locus_tag="PLZ72_04955"
     CDS             15786..17204
                     /locus_tag="PLZ72_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35679.1"
                     /translation="MTTSHIGQEQSRSRRTDAAAADAAAGGSGPLGRVVAKVQKVAAA
                     VSAEVVQSMTSFLLQIIAVRELSAAGLGVFALLYSCVILSTAVTTGMVGDSLTVLDRH
                     DASVRGGLQAVALTIAVVGGTVACVLSGATGLISWDLAPLFGLAATIFVLEELIRRML
                     MASLRFWFVVAVDTTCLVVTAFWLGLFKVLDGQLVMRDLLSSLVVAQLCGLLVGVVLL
                     PRAERWWATMRASRWREVVRYGRWRAAQQAVRPAMMAAVRTIVIVAVGTTLFGELEAA
                     RVFAAPAMLIVNGVGSFLFASYAARRRSTLRELIPLADRGTGMLVGLSLVLGAVSVAL
                     LPAVGDVITSGKFELDPIAVFGWSVYAAASAVLMPYGSLAAVRGRHAAMMGLRVVEAI
                     VSLTSLFLLLRALHVDVSYVPYALALGSLAMGVVARQMILVPTAREEERAAARRERRR
                     NARRAQGAVRSAAPGGPRSTAD"
     gene            17234..18541
                     /locus_tag="PLZ72_04960"
     CDS             17234..18541
                     /locus_tag="PLZ72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA35680.1"
                     /translation="MARSQRTLSGRFLGLVDDWWLAVAGVAFIVGSDFKYRVRPPGQA
                     LGGGIDAAIMVEMLLYGLVAGYLLLDRFRVPRVRRIPAPLYLAACFVGLAVLSLMYTP
                     YLQYGVVRCVQATILLAVVVIAAMDGDRSHFHRFAHLYLLLILAGVGYGLVRPSPPLK
                     ASQAGRFTWLAVHPTVSGAMMCLAAVVAVGYVVGGRSDRPGPRWPPLLYWGILAVVAG
                     GGLAGHTRGAILGGICGAAVVLVTTRRGRAVVEVGLVGLVLVAGVAIMASDKVTTYFE
                     RGDTSAQLTTLSHRTELWSVAFESIEKKPLFGNGITASRGIFYEATGLGGGHNGAINV
                     LVELGAVGLLVWAALLISLLLGIRHLPRNGPRDLQLDRAMLLGVMTFLLVDSIFYEGI
                     GSVANVASTWLFMCIGWYVVARRDGRRPIAAAEVESVPVSAGG"
     gene            complement(18579..20441)
                     /locus_tag="PLZ72_04965"
     CDS             complement(18579..20441)
                     /locus_tag="PLZ72_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA35681.1"
                     /translation="MKHRIQIAVITIGATLLAAACSGAPAGSESDDSGSALADSSTRR
                     AAATVPARPTVPPSAPALAPATAHGSGTTLGSTSYPVPASAIVVSPAGDDAANGSVAA
                     PLRTLGKAIAIARTGATIVVRAGTYHESVTIPSGRTVTIQNWPGEVVWFDGSDAVTGW
                     VADGSTWRRDGWNVSFDHSPTYTRGASDNSADQWRFVNPQFPMAAHPDQVWVDGAPQA
                     QVASAVEVGPGRFHKDDAGSRLRIGTDPSGHEVRASTLIRAMSIRATGSTVRGIGFRR
                     YSPSVPDMGAITVEAPGVTLEHLVVSDMATTGVSVVSTGATLRNVEVARSGMLGIHAN
                     YADGLRLLGVVSSANNTEHFNSAPVSGGFKVTRTRGLTVTGCVFRDNLGPGLWTDQAV
                     YDITVTGCDMIANSGHGTSLELSAKAVFADNLVSGNGGNGIKINDTSEVVLVNNTFVG
                     NNRPVNIVQDSRRGNDPSVPGHDPRRPFPDPTMPWVNGPVLVQGNLIAAPSAGNCLLC
                     VEDYSRTFSAEQMAITSPGNAYHRGAANSPTWLVVWSRGATNPAVLNDLGAFRTATGQ
                     EASGQMVTGAPIVDAAGRPTPSGQALDPSAPELPASVAALIGRPAGTRHFGAFR"
     gene            complement(20655..21107)
                     /locus_tag="PLZ72_04970"
     CDS             complement(20655..21107)
                     /locus_tag="PLZ72_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNA35682.1"
                     /translation="MPGSDQPSRNERRVGYAPGVYDLFHVGHLNMLRNATQFCDYLIA
                     GVVTDEMSQRTKGVRPVVPIDERLAIVGAIRFVDEAVAEDVPHKLEMWERLHFDVIIK
                     GDDWRGTDKGDKLESDFAAHGVDVAYVPYTQRTSSSMLRRILERELDL"
     gene            21263..>22130
                     /locus_tag="PLZ72_04975"
     CDS             21263..>22130
                     /locus_tag="PLZ72_04975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35683.1"
                     /translation="MNFAFSEEQEELRKVVRDFLNAKSDEPTVRELMDTEAGYDAAVW
                     TQMAEQMGLQGLVIPEEFGGSGYSFVELIVVLEEMGRRLLCAPYFSTVVLAGQTLIHS
                     GDDAAKAAHLPGIASGETVATLAYTEPNGRWDESAVTATATADGDTWKINGEKLYVLD
                     GHTADLIITAARTDAGVSLFAVAGDAPGLTRTALSTMDQTRKQARLQYADVPGTLIGT
                     DGGGWDVLSTVLDLAAVALAAEQVGGSQEVLEAAVQYAKDRVQFGRPIGSFQAIKHKC
                     ADMLLEVESAKSA"
BASE COUNT         3240 a         7635 c         7647 g         3408 t
ORIGIN      
        1 ctcggggcgg ccccacagca cgccgattcc ggtcggcccg agcatcttgt gtccggagaa
       61 cgccaggaag tcggctccga gggcgtcgac gtcggtggcg acgtgcggga cgtactggca
      121 cgcgtcgatg cacgccaggg ccccggccgc gtgggcggca tcggtgagca tccgcaccgg
      181 ggtgatggtg ccgaccacgt tcgacatggc cgacagcgcc aggagcttcg ccccgtcgag
      241 caggcggtcc agatcggtga ggtccaagcg gccgtcgtcg gtgaggggga tccaccgcag
      301 ctctgtgccg cgctcggcgg ccagctgctg ccaggggacg atgttggcgt ggtgctcgag
      361 ctcggtgagc accacggcgt cgccggggcc gaggttggcg tcaccccagg aacgggccac
      421 caggttgagc gactcggtgg cgttcttggt gaacaccacc tcgtcgggtg aggtggcgcc
      481 gatgaagcgg gccaccctgg cccgggcgcc ctccatggcg ttcgtcgccg cctcggcgag
      541 ggagtaggcg gcccggtgga cgttggcgtg ggtcgtctcg tagtagcgat ccatcgcctc
      601 gatcaccgcg gcgggcttct gcgaggaggc ggccgagtcg aggaacacca ggggtcggtc
      661 gtgcacctcg gtcgacagga tcgggaagtc ggcccggacc ttgacgacgt cgaggtcgtt
      721 cgggccgtgg ccgtccgtgg tgcgcgcgct ggagctgggg gcggtgctca cgcgacggcc
      781 tccggttccc ggtaggcgtc gaagccctcg gactccaggc gccgggcgag ctcagggccg
      841 cccgattcga cgattcgtcc gtcgacgagc acgtggacga cgtccggcgt gaggtgctcg
      901 aggagccgct ggtagtgggt gatcaccagc acaccgagct cgggccggtc cttgcgcacc
      961 tcgtcgaccc cgtcggcgac gacgcgaagt gcgtcgatgt ccagcccgga gtcggtctcg
     1021 tcgaggatgg cgacgtcggg ttccaggatc gccatctgga ggacctcgtt gcgcttcttc
     1081 tctccacccg agaagccctc gttgaggaac cggtcggcga agctcgggtc catgtcgagt
     1141 cgcttcatcc actccattat cgcgacgcgg atctcgagca cggacagatc gatgcccttg
     1201 cgctcggaca gcgcctgacg caggaactgg cgcaccgaca cgccggggat ctcctggggg
     1261 tactggaacg ccaggaacat gccgtggcgg ccgcgctggt ccggggtcca gtcggtgacg
     1321 tcgtcgccgc ggaggtgcag agacccaccg gtgacggagt actcgggtga ccccatcagc
     1381 gtgctggcga gcgtcgactt gcccgatccg ttgggaccca tgagagcgtg gacctcgccg
     1441 gggtggatcg tgagcgacac gccgtggagg atcggggcct cgaggttctc ggcaggggcg
     1501 gcgtgcaggt cctcgatacg gaggaggggg gcgacggcca tgaggtgagt ctatttcccc
     1561 tacgcggatg gtggaaatag gtccgggccg ggccgtggcg gatgggagcg accagatctg
     1621 agtgggagcg ccttgtctgg caccatggtc gcccatgccc gtgacgttcc tggcccacca
     1681 ggccccggtg ctacctctca agcgccggtg gcccggcctc gacggggtcg ccctggtggt
     1741 cgggagcact gttcccgacc tggctcgggc caccgagcgg ttgccggtcc tgtacctgta
     1801 cgggacgccg acctggttcg acggccaccg ccccgaccag ttggtgagct ggtgcctggt
     1861 ggtgggcctg ctgatcacgt ggtcggctcg tcgcctggtc ctgccccgac tcggtggtca
     1921 ccttcccgac ctcggcgact tccacctgcg ggatctgcgg ctggtcggcc ggacccgtca
     1981 ccggtggtgg aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctctgc
     2101 ctcatcgccg ccaccaggcc ccgccccgac ctcggtgcgg acaccgccgc cgtggcggac
     2161 tcagaagccg cggtcgatcc actcctggag gtggggtgac tcggccccga tcgtcgtgtc
     2221 gtcgccgtgt ccggggagca cgagcgtgtc gggtgccaac cgggagaaca gacggtcttc
     2281 gatcgacgtg atgatggtgg ggaagtcgcc gccctcgaac tgggtcgccc ctggtccgcc
     2341 ggggaacagg gtgtcgccgc tgaacaggat cggcttcccc tccacacgga agcagatgct
     2401 gccgggggtg tggcccgggg tgaggaccgt gcgcagccgc aggtcgccga cctcgatcac
     2461 ctcgtcgtcg ccgatcagct cgtcgtagct gggaagcatc cccgcgtcgt cggcggtcac
     2521 tcccaccgag tatccggcgt cgcgcatctc ggggatggcc tggatgtggt cccagtgccc
     2581 gtgggtctcg atgacccggc gtacgccgag cgaggaggcg aggtcgagca gcacgtcgtg
     2641 ctcgttggcg gcgtcgatga gcacggcgtc accacggtgg cggcagcgca ggacgaagac
     2701 gttgttgtcc atcggtccga cgacgacctt gtggatctcc atgtcggtgt cggagacgtg
     2761 gagggttccg gtgccgtcgg tcatggatgg agtctcgcgc cggtgccgcc gtgacggtcg
     2821 tgcgggcgag ggcctcagcg cgagccgacg acgtcgaagc ccagggcgag ggcgacaggg
     2881 atctgggtgg gatcgaacgt cacgaaggtg acggggcggg ggaggcggtc cgctgcggcc
     2941 aggtggatgg cgtcgacggt gcgcaggggt tgggtccgtc ccagttcggc ggcccggtcc
     3001 aggcacagct ggtcgaccgg caccacgttg atccgctccc agtcgtcgcg cagggcccgt
     3061 cggagctcgt cggcgcgcga cggatcgtcg cccagtcggt cgaccagcat gagcgcttcg
     3121 gacagggcga gagcgctggc gcaccagtcc ggatcggcgg ccatcgctgc cagcgcgatc
     3181 ggccgggtgg gtccgtcgag ataacgcgac aacaggccgg tcgtgtcgag tgcgaccgtc
     3241 accgcgaccg tccgatcatc gccctctcac ctctcggacc agttgatcga tgcgggtgcc
     3301 cgcccacaac ggcaggacgg tggacggatc cggacggtcc tggcgacggg cgagcaacac
     3361 ctggcctcgg gcagccagat cggccagcga cgtctgccgg tcggcggctt cgatcgggcc
     3421 cagctgagcg acagggcgtc cgccgacggt gacgatcacc cgatggccgt ccccggccct
     3481 gcggacctgg gttgccacgt cggccctgag ctcccggatc ccgatctggt ccataccggc
     3541 gaggctaccg accccccggg atggccccgg tagcgttgcc ggtatacttc gtcgttctgc
     3601 accgggtgga gagagaacga gacgtggctg acgagacgaa gacaccatcg ggagccgacg
     3661 agtcgccggc aggcacccgt cgcggatcgg ctgcaaaggg ttcggccggc gaaggcacac
     3721 ccgacaagga gacggcgtcc gggggtgctt cgacccggag ggcaacttcc aaggcctccc
     3781 cgacgcctga ggcgccctca ggtcgtacgt ctgcccgcac ctccgatcgg gaggtcaagg
     3841 gatccgggga tgatgccgcg cctgcggccc cgcccgtcgg tcccgcaccg gcggcgactc
     3901 ctcgagccgc ggatgcagct ccggcggtca gcgccggagg cgacgctccg gtcgggctgt
     3961 tgagcgccat gtggcgctac aagggcatgt gcgtcgtgat cgtcgtggtg tgcgtcctgg
     4021 ccagcacggc gatcgggttc ctgctgactc cggagccgag cgccaccgcg accatcgctc
     4081 tcaagacgcc gagccaggac aacgtgcttg ctccgggctc caccggtgac gcctcgctcg
     4141 cgcgctacac ggcgcagcgc gcccggttcg tcaccagcga cgcagtgctc ggcaacgcgg
     4201 gcgccaactt gggcgtcgac gatctcaacg agctccgcga ccagctcgag gtcgagccat
     4261 ccaccgattc caacatcgtg acgatcactg cgtcggggac cacgtctgac gatgccgtcc
     4321 gcctggccac cgaggtcacc cgtgcctacg gcgaagagac cgagaagcag gtcaacgacc
     4381 tcaccgacgc cgccctccgt tcgatcgacg agagcgcagg gcaggttcgg gccacgatca
     4441 ccggcagcaa cgccgcggtc aacgaggcgg cggcttccac gctcggtcag ctccagcaga
     4501 aggcggccac catccgcacc tcgtcggcgg tcctcggcga cggcgtcgag ttcgtggtca
     4561 accccgacga gtcctcggtg agcagccagc agctgcccat ccgcgaggcg gcactcgggt
     4621 tgatcctggg tctcgtcatc gccggcacgg tcgcctacct gcgagccgac agcgacgcgg
     4681 cgaagcagcg gcgggcctga accgcccgat gcggatcctc gaggtcgggc ccccggttcg
     4741 tcacgagcga cggacatgac ctccggagca ccacggccgg ccggagtccg tccggccgag
     4801 gacgagtcct acggcgacac agtccgccgc ctcgcagcgg cccagaagtc gggccggggc
     4861 gcgccggcgt actcgcggtt cgtcaaccgc aagctgggcc ggctgttcgc cgcggccggg
     4921 ttccacctcg ggctgacccc gaacgggatc actttcatca gcgcggcctt ctcgttctcg
     4981 gggctggttc tgctcgcagc gatgaggccg agctggtggc tcgggttcgc ggtcaccgcc
     5041 ctgctcgtcg tcggctacgc gctcgacgct gcggacggcc agctcgcacg attgcgcggc
     5101 ggcggctcgg tcaccggcga gtggctggac cacatggtcg actgcctgaa gatctcgacg
     5161 ctgcacatgt gcgtgctgat cagctggtac cgcttcctcg tcaccgatcc acggacggcc
     5221 gacccgatcg acgagcggtg gctcctcgtc ccgatgggct acgcggtcgt cgctgcggtg
     5281 tcgttcttcg cccagatcct caacgagcag ctggtccgca acgcggggct gcgatccgcg
     5341 tcaccgacct cggccgcatc ggccgacgcc gggacgccct cgatcctggt gtcgttgctg
     5401 aaggtgccga ccgactacgg gctgttgtgc ctgatcttcc tcctgctcgg cgcgtcggaa
     5461 ctgttcgtcg gcgcctatac cctcatgttc gtcggcagcg ccgcctacct catggcggcc
     5521 tcggtgaagt ggttcggcca gatgaagggc ctggaccgag gagcgtcatg acggtcagtc
     5581 cccaccacga tcgcctcagg aagggccgtc tcgctcggcg cgtcgggtcc tcggccgtgg
     5641 tcgcgctctc gatcctcgtg ccctacggat gctcgtcgag cgacgactcg aacccgaccc
     5701 gcagcaccga ggcggcagtg acgaccgagc cgggcgacgg caccgtggtc ggacccacca
     5761 ccaccggggt tcccgccacg gcgcccgaca ccgcgcccgt cgccgacatc gtgaaggagc
     5821 cggtcgctcc cggcaccccg gctccgctgg gtgccggcgt gtccgtcgtc ctggtcgact
     5881 ccgagaatct cgacgtcgag gcccgggtcc ccggtgagac ggccgggccc gccgtggcgg
     5941 ctaccctcga ggttcggaac gactcgtccg agcccttcga cctgtcgtcg ctcgccgtca
     6001 ccgccaccta cggcgacggg accccggcga tcggcaacga ctccgaaccc gcccgtgcgc
     6061 tgaccggcac tgtcaaggcg ggagcctcgg ccaagggcgt gtacgtgttc cgggccccgg
     6121 tgaaggcagc cgacacggtc atcgtcgagg tgcagtcggc ggaccggccc aacgtcgtga
     6181 ggttccgggt cgtctgaacc tggtggtccg gccggcgggg cccgatgact tgccccgcgg
     6241 acgttcgtga tccaccatga acccggtggt cgcgccggtg tccggcgcga cgtgagggag
     6301 gatctgaaga gcggtcgagg ccttcggtcg ccgatgctcc cttggcggtg gttgctggga
     6361 gatggcgatg gacaccaaga gctcgtgctc gacgtcgggg gcgttccgct cccgggccct
     6421 gcgggcggtg gcggccgttt cggccgccct gatggcggtt tcggtgctgg tcgttgctcc
     6481 ggcctcggcc gacagcgcac cgacggcgcc gggcacgccg acgacggtca ccgccgactc
     6541 ccttcccacc gttcagatca acggcgtggt ctgggaccag gtcgtggtgg gcaacaccgt
     6601 ctacgccacc ggcagcttca cccaggcccg acccgcgggt gctgcggcgg gaaccaacga
     6661 gaccgcccgg tccaacatcc ttgcgtacaa catcaccacc ggaaacctga tcacgacgtg
     6721 ggcaccgtcg ctcaacgccc agggccgggc catcacggcc tcggcagacg gctccaccat
     6781 ctacgtcgcc ggtgacttca cctcggtcag cggcaccacc cgcaaccgcg tcgcggcgct
     6841 caacgcaacc accggagcgg tcatcagcgg cttcaacgcc aacgtcaatg cccacgtcaa
     6901 cgacctcgag gtgtccggat ccaccctgta cctggtggga gcgttcaccg tggtcggcaa
     6961 ccaggcccgc tcccgcctgg cctcggtcag cgcgtccagc ggcgcggtga ccgcgtgggc
     7021 tccttcctcc aacggagagg tccgcaccct gacggtgccc gccggtacgg gcaaggtcgt
     7081 cgtgggcggc cacttcgaga ccctcaacgg ctccacccag accggcatgg gggcgctcga
     7141 cgccagcagt ggttcggtcc tgtcgtggcc ggccaacacc gtggtgaaga accacggaac
     7201 cgaagccgcg atctggagcc tcaagtccta cgggtccgtc gtcttcggca acgggatgtc
     7261 cttcgcgtcg accggcaact tcgaagcgac cttctcctcg gataccaacg gcaacctcat
     7321 ctgggtcaac ggttgccgag gcgacaacta cgacaacgtc gcggtcggca cggtgctcta
     7381 cacggtgggt cacacccatg actgctccag cgccggcggc ctcccccaga ccaacccgga
     7441 ctggaccttc caacgggctc tggccatgac cacgtacgcc gatccttccg gcgcggtcaa
     7501 cacctcgaag tggttcacag ggcgtccggt ctcgcagctg ctccactggc tgcccaccgt
     7561 ggccgccggc accttcaccg gtcagggaca gggcgcctgg accatcgagg gcaacagcca
     7621 gtacgtcgcc atgggcggcg agttcccccg cgtgaacggg gtcaaccagc agggcctcac
     7681 gcgcttcgcc gtgaccggac tggctccgaa caaggagggc ccgcaggact acgaccacct
     7741 gacgcccaag ctggtcggga tcgctcccgg ctcggtgcgc gtctcctgga cgacgacctg
     7801 ggaccgcgac aacgagcgcc tcacctacga ggtgctccgc ggcaccacgg tcatcgccac
     7861 gaccgaggcc gactccaact ggtggacccg tcccgagatg gccttcatcg accgcggggc
     7921 cacccccgga tccacccagt cctaccgggt ccgggtgcgt gacgtgttca acaacatcct
     7981 cccgtccgcc tccgcgtcga tcacggtgcc gagcggtacg ccgtcggtcg gggcgtacca
     8041 ggctgcggtc gcggccgacg gcgccaaccg ctactggcgg ctgggagagg gctcggggtc
     8101 gaccggctgg gacagcgcca acggcaacga cctgaccctg tcgggcgtga cccgcaacgt
     8161 cggcggagcc atctcgggcg acgccgacac cgccagcacc ttcgacgggt cggccacggt
     8221 tccgggaacg accaccgggt ggatctccgc cccgcagacc ttcaccattg aggcgtggtt
     8281 caagaccacg accaaccggg gcggcaagat catcggcttc gggagctcca gctcgggcag
     8341 cagcgccacc aacgaccggt cggtctacat ggacaacagc ggccggatcc gcttcggtgc
     8401 gacgtcggct tcggcgctca ccagcgggac cgggtacaac aacggccagt ggcaccacgt
     8461 ggtcgcgtcc atgggtccct cggggatccg actggtactc gacggcaacc aggtcggagc
     8521 gaactcgtcc atcaagaccg ccggctacta ctggggctac tggcgggtcg gtggcgatca
     8581 gctcagcggc tggccgtcgc gtccgaccag caactcgttc gccggcaccc tcgatgaggt
     8641 cgctgtctac ccgaccgagc tgaccgtcgc gcaggcggcc aaccacaacg ccctgggccg
     8701 cggcgcggcc ggcaacgtcg ccccgacggc gagcttcacg gcttcggcgt cgggtctggt
     8761 ggcgtcggtg gacggttctg cgtcgtccga tgctgatggt tcggtggtgt cgtatgcgtg
     8821 gaactggggt gatggttcgg cggcgggttc gggtgtgtcg gcgacgcaca cgtacgcggc
     8881 ggcgggtacc tacacgatca cgttgacggt gaccgatgac gacggggcga cgggtacgac
     8941 gacccggtcg gtgaccgtga ccgctcctcc gtcgaacgtg gcgccggtgg cgagcttctc
     9001 ggcctccgcg tcgggtctgg tggcgtcggt gaacggttct gcgtcgtccg atgccgatgg
     9061 ttcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttcgg cgaccaacgc
     9181 tccggtggtc ctgtcggtcg acgacctcga cgcccgaccg atctgatctg gccttccgga
     9241 ggccgctccg gacgctgtgg agacccgtcg tggtgggccg aatggcccat gacgacgggt
     9301 ctcgtcgcgt ccggacccgg tccgagcggc catcgacgca ggatctttgc tcagtgtgga
     9361 tccgcaacga ccgacgttag gttcatcggt gggcgtccgc gatcccgcgg cggcccttga
     9421 gagaagcccg ggtccgagag gccccgctgg gagagcacag atgtcagtca cccccgagtc
     9481 gtccccccgt cggtggatcc gaagcgtcgc cggtgcagtt gtcgccttga tggcgtcgtc
     9541 gatcgctgcg gtcgccctgg gtgtcggtgt ggccggagcc gacaccgccc cggtcgatcc
     9601 cacggagccg gagaccgtcg cggctgacag cctccccacc gtccagatcg acggtgtggt
     9661 ctgggaccag gtcgtcgtcg gcaacacggt ctacgcgacg gggtctttca cctcggcccg
     9721 gccggccgga tccgcagcgg gaacgaacga gacgccgcgg tccaacatcc tggcctacga
     9781 catcaccacc gggaacctga tcacctcgtg ggcgccgacg ctcaacgccc aggggcggac
     9841 cctcgaggct tcggccgacg gcaacacgat ctacgtcgcc ggtgacttca cccaggtcaa
     9901 cggcacgacc cgcaaccggg tggccgccct ggacgccacc accggcgtgc tcaggccctt
     9961 caaccccaac gtcaacgccc acgtcaacag cctcgccctc tccggcgaca ccctctacgt
    10021 cggtggggcg ttcaccgtcg tcggcaacca gggccgttcc cggctggcgg ccgtcgacgc
    10081 cgcctccggt gcactccgcg agtgggcacc cgtggccaac ctcgaggtcc gggcggtggt
    10141 cgtcccggcc aacaagggca aggtggtggt cggtggtcac ttcgacgaca tgaacggtgt
    10201 tccgacccgc ggcctggccg ccctcgatcc cacaaccggg gcactcgtcc cgttcccggc
    10261 caaccagatc atccagaact ggggtgcgga cgccgcgatc tggagcctgt ccaccaacga
    10321 cgaccaggtc ttcgggaccg gcttcaccta cctggtcaac aacgacccga ccaccggtgg
    10381 gaacctggag tcgaccttcg cggccgacgc caccaccggt gagttgatgt gggtcagcgg
    10441 ctgccgaggg gaccactacg acaccgcccc gatcggcaac gtcctctaca acgtcagcca
    10501 cgcccacgac tgcgcgccgg tgggcggcca tccccagacc agcccgtgga cgttccagcg
    10561 ggcccaggcc gagacgatct accccgaccc ttccggcgcg gtgaacgtcg gcggcaagtt
    10621 caacggtcgg ccccgcgccc ggcagttgca ctggctgccc acgatggcca tcggcacgtt
    10681 caccggccag gcccaggcgg cctggaccgt cgagggcaac agccagtacg tggtcatggg
    10741 cggcgagttc ccccgcgtga acggggcgaa ccagcagggc ctggcccgat tcgccgtcaa
    10801 gtcgatcgct ccgaacaagg acctcctcca gggctacccg gagttcacgc cgacgctggt
    10861 cccgatttct gccggttcgc tgcggatctc ctggcagggg gtctgggatc gcgacaacgc
    10921 ccagctgacc tacgaggtgc tccggggggc cactgtcaac acctcgacgg tggtggccac
    10981 ccggacctac tcaccgtcca actggtggta ccgcccgatg ctcgcggccg tcgacgcctc
    11041 cgccgccccc gggtcgaccg agacctaccg ggtccgggtg cgcgacgcct tcgggaacgg
    11101 attcgtcagc ccggcggtca ccgggaccgt cccggcgggc accccgacgc cgagtccgta
    11161 ccgcgacgcc gtcatcgccg actcgcccgc caagtactgg cgcctcgggg aggccgccgg
    11221 cacgatcggc tacgaccagg tcggcgctga cgacctgacc ctcaactcga acaccacccg
    11281 tggcgtggtc ggtgccctga tcggcgactc cgacacgtcg accaccttcg ccggcggcac
    11341 cgctccggcc tcgaccaatg ggacagcggt caacggacct cagcagttca ccgtcgaggc
    11401 gtggttcaag acgacgacct cgtcgggcgg caagatcgtc gggttcggca agtcgtcgac
    11461 cggatcgagc accagctacg accgtcacat ctacatgacc aacggcggtc agctgcgctt
    11521 cggcgtgtac gacggcagca cccgaaccgt ggcgtcgtcg gccgcctaca acgacggcca
    11581 gtggcaccag gccgtcggcc agctcggccc ggccggcatc aagctctacg tcgacggcaa
    11641 gctggtcggt gccgacgcgt ccgccaccag tgcggaggcg ttcgccggct actggcgcat
    11701 cggtggtgac gccctcggtg gctggccgtc gcaaccctcg tcggccaact tcaacggcac
    11761 catcgacgac gtctccatct acggcggcga gctcacccag gatcagatcc gggcccactt
    11821 cctggccagc ggtcggaccc cgggctggcc caccgtgccc gccgatgcgt acggcgccgc
    11881 ggtgtggaac gccaacgctg atctctacct gcgactcaac gagacctcgg gctccaccgc
    11941 ggtcaaccgc atgacgctgg acccgggtgc caccgcgagc ggcaccgtgt gggggggagc
    12001 cgcctcgccg gccgatccga gtggcacctc ggtcgagttc aacagcaaca acgaccgggt
    12061 cgtcggcacc cagcagtaca cgaacccgac cacgttctcg ctcgagacgt ggatccgctc
    12121 caccgacacc aacggcgggc gaatcatcgg cttcggcgac tcgaccggtc agaccagctc
    12181 caactacgac cgccatctct acctgctggc cgacggccgg ctgcgctacg gcgtgtgggt
    12241 cggttccgcg gccaccatcg actcgcccca ggcggtcaac gacggtcagt ggcaccacgt
    12301 cgtggtgacc cagcagcccg gtgagcagaa gatgtacgtc gacggcgtcg cggtcgcctc
    12361 cggaacggcg tcggcccaac agaactactc cggctactgg cggctcggag cggacaacat
    12421 ctggtccggg gaccgccact accggggcct gatcgacgag gccgcggtgt acggctcggt
    12481 gctgtcgccc gaggcggtgc tgtcgcactg gaccgcggcc gggggcctgg ctccgaacgt
    12541 caacccgacg gcgtcgttca ccgccggagc ggtcgacctg gcggtcacct tcgacgcctc
    12601 cggctcgagc gacccggacg gcacgatcgt ctcctacgac tggaacttcg gcgacggagc
    12661 caccggtagc ggcatcaact ccgcccacac ctacggcgcg gccggtacgt acaacgtgac
    12721 cctcaccgtc accgacgacc gcggcggcag cgccacggcc acctcgcagg tgacggtgct
    12781 ggcgccgaac gtgacgccga cggccgcgtt cagcgcggtg gtcgaccacc tcgacgtcga
    12841 cttcgacgcc agcatttcca acgatccgga cggcaacgtc gtcgcctacg catgggactt
    12901 cggtgacgga acgccgacgg tggaggggat cgcccccacc cacacctacg cgggagcggg
    12961 gacctacaac gtgacgctga cggttaccga cgaccgtggc ggtgtcggaa ccgtgacact
    13021 gcccgtcacc gccacgctgc cgcccaacgt cctgccgacc gcagcgttca gctcgggcca
    13081 cgtcgatctc accgcgacgt tcgatgcctc ggcgtcgagc gacccggacg gaacgatcgt
    13141 gtcccacgcc tgggacttcg gtgacggaac ccagggcaac ggcgccacga cggcgcacac
    13201 ctacctggcg gcgggcacct acgacgtgac cttgaccgtc gtcgacgacc gcggcggcca
    13261 ggacacagtg acccatgagg tcaccgtgac cgatcccccc aacctcgctc cgaccgcgtc
    13321 gttcaccttc ggtcacaccg acctgaccgc caacttcgac gcctcgctgt cgtcggaccc
    13381 cgaaggccgg ccgctcagct acgcgtggac cttcggtgac ggcagcggga cctcgacgag
    13441 cgtcgctccc acctacacct atgcggcggc gggcacctac ggcgtgacgc tgaccgtgac
    13501 cgacgatgcc ggagccaccg cctcggtgac ctggccggtg accgtgaccg acccgccgcc
    13561 ggccaacgtc agtccgacgg cgtcgttctc gtcgaacacc agcttcctga ccgcggacgt
    13621 ggatgggcgt gggtcgagcg accccgacgg atccatcgtg agctggtcgt ggaactgggg
    13681 tgacggcacc gctccgggtt ccggggccac cgcctcgcac gtcttcgccg gtgccggcac
    13741 ctacacggtg accctcaccg tcaccgacga cgacggtgcc atcgggaccg tcaccggcca
    13801 ggtgaccgtc tcggcgcctc cggcgaacgt cgcacccacc gcgtcgttca ctgcctcggc
    13861 caacgacctg accctgtcgg tggacgcatc gacgtcgacc gaccccgacg gttccatcgt
    13921 gagctggtcg tgggtctggg gtgacaacac cgcagccgga gcgggctctg ccgcgaccca
    13981 cacgtacgcc ggtgccggca cctacacggt gacgctgacc gtcaccgacg acgacggtgc
    14041 gaccgacacg accagtcgaa cggtgaccgt gaccgctccg gtggtgtcca actacgcgtc
    14101 cgacaccttc agccgaacgg tcagcaacgc gctggggaca gcggacgtcg gcggtgcctg
    14161 gaccaccact ggctcggccg ccagctactc ggtgtccggc ggcctcggcc gggtctcgac
    14221 gaccgccggg gcgagccgca ccatcaccct cggctcggtc gccgcgaccg acaccgatct
    14281 ccgagcccga ctgggcttcg acaagaccca gaccggaggt ggctcctacg tcggcatcat
    14341 cggacgtcgg gtgaactcca ccaacgacta ccggctcaag ctgcgcttcc agtccaacgg
    14401 gtcggtcacg gcccagctcg tgcggatcgt caacgggacc gagaccgtcg ttcagaacct
    14461 gtcgacggtt cccgggctca ccgtcaacgg cggcgagttc gtccgggttc gactccaggt
    14521 caccggcacc aatccgacca acctggccgc caaggtctgg aaggacggga cggccgaacc
    14581 gacggcctgg cagctgcagg cgaccgatgc cagcgcctcg ttgcaggcga acggttcggt
    14641 cgggatctgg gactacctgt cgggttccgc gaccaacggt cccgtcacga tgctgatcga
    14701 cgacgtcgtc gccgggccgg tcggtggcgg gacgccgcct ccgaccaacg cggcgccgtc
    14761 ggccgcgctc acgtcgtcgg tcacgggtct cgacgtggcc gtcgacggct ccgggtcctc
    14821 cgacggtgac ggaacggtcg agtcgtactc atggaactgg ggtgacggga ccccggccgg
    14881 atccggggtg accgccgcgc acacctacgc ggttgcgggc acctacacga tcaccctgac
    14941 ggtgaccgac gacgacggcg ccaccggaac cagcagctcc acggtcgtgg tgacgagcgg
    15001 gcccgccccg accaacttcg tcgaggacag cttcggccgc gacacgacca acggatgggg
    15061 cagtgccgag atcggcggtg cctggtccac gacgagcacg gcgtcggcct actcggtgtc
    15121 cggcggtgcc ggtcgcatca ccatgaacgc cggagcaggt cgctacgcca gcctcaactc
    15181 cgtgacccag accgcgtccg acgtccgggc ttcgctcagc ctcgacaagg cccagaccgg
    15241 cggtggaacc tacatggccc tcgtcggccg ccgtgtcgac ggttccaacg actaccgggt
    15301 caagctgcgg gtgcaggccg gtggagcggt gacggcccag ctggtccggg tggtgaacgg
    15361 caccgagacc gtcatccaga gcgtggcgac cgtcccgggg ttgacctgga acgccgggga
    15421 ggtcctccac gtccggttcc aggtgagcgg taccggcacc accaacctcg cggccaaggt
    15481 ctggaaggac ggcacggccg agcccaccgg ctggacgctg tcgggggcgg atcagaccgc
    15541 cgctctgcag tccgccggtg gcgtgggaat ctggtcgtat ctctccggat cggcgaccaa
    15601 cgcgccgatg accctcacgg tggacgacct cgtcgccggt ccgctcggct gaggtcgtcg
    15661 tcctccgccc gtcgtcgatg ggtccgtcgg gagcctccgg ttcccgacgg acccacgacg
    15721 ttcggtacgg tcaaccccgc tccacctgac ctcgaccgcc caccacgccg gacaccccac
    15781 tcaggatgac gacctcgcac atcggacagg agcagtcccg gtcccgacgg accgatgccg
    15841 ccgctgctga tgccgcggct ggtggatcgg gacccctggg ccgcgtcgtc gccaaggtcc
    15901 agaaggtcgc ggcggcggtg tcggccgagg tcgtccagtc gatgaccagc ttcctgctgc
    15961 agatcatcgc ggtccgcgag ctcagcgccg ccggcctggg cgtgttcgcc ctgctctaca
    16021 gctgtgtgat cctgtcgaca gcggtgacca ccggcatggt cggcgactcg ctcacggtcc
    16081 tggatcgcca cgacgcctcg gtccgtggtg ggctgcaggc ggtggcgctc accatcgccg
    16141 tcgtcggtgg gaccgtcgcg tgcgtgctgt cgggagcgac cggtctgatc agctgggacc
    16201 tggctccgct gttcggtctt gcggccacga tcttcgtgct ggaggagctg atccgccgca
    16261 tgttgatggc gtcgctgagg ttctggttcg tcgtggcggt cgacacgacg tgcctggtgg
    16321 tcaccgcgtt ctggctgggt ctgttcaagg tgctcgacgg gcagttggtc atgcgggacc
    16381 tgctgtcgtc gttggtggtg gcccagctgt gcggcttgct ggtcggagtc gtcctgctgc
    16441 ccagggccga gcggtggtgg gcgacgatgc gggccagccg gtggcgtgag gtcgtccgct
    16501 acgggcgctg gcgggcggca cagcaggccg tgcggccggc catgatggca gcggtgcgca
    16561 cgatcgtgat cgtggccgtc ggcaccaccc tgttcggcga gctcgaagca gcgagggtct
    16621 tcgctgcgcc tgccatgttg atcgtcaacg gcgtcgggag cttcctgttc gcctcgtacg
    16681 cggcgcgtcg gcgcagcacg ctgcgggagc tgatccccct ggccgaccgt ggcaccggga
    16741 tgctggtcgg gctctccctg gtgctcggag cggtgtcggt ggcgctgctg cccgcggtcg
    16801 gcgacgtgat caccagcggc aagttcgaac tggatccgat cgcggtgttc gggtggtcgg
    16861 tgtacgcggc ggcgagcgcg gtcctgatgc cgtacgggag cctggcggcg gtccggggac
    16921 ggcacgccgc gatgatgggt ctgcgtgtgg tcgaggcgat cgtttcgctc acgtccttgt
    16981 tcctgctgct gcgggcgctg cacgtggacg tgtcctacgt cccgtatgcc ctggcgctcg
    17041 gctcgctcgc gatgggtgtg gtcgctcgtc agatgatcct cgtgcccacg gcgcgggagg
    17101 aggagcgggc ggccgctcga cgggagcggc gccggaacgc aaggcgggcc cagggtgccg
    17161 tccggtcggc tgctccgggc ggaccgcgct ccacagcgga ctgaggcccg gtcggttcac
    17221 ctaacctggg acggtggcac gctcgcagcg cactctctcc ggccgcttcc tcggtctggt
    17281 cgacgactgg tggctcgcgg tcgcgggggt ggcgttcatc gtcggcagtg acttcaagta
    17341 ccgcgttcgc ccccccggcc aggcgctcgg cggtggcatc gacgccgcga tcatggtgga
    17401 gatgctgctg tacggcctgg tcgccggcta cctgctcctc gaccgcttcc gggtgccgag
    17461 agtccgtcgg atcccggcgc cgctgtacct ggccgcctgc ttcgtgggct tggccgtgct
    17521 gtcgctcatg tacacgccgt acctccagta cggcgtggtg cgctgcgtgc aggccacgat
    17581 cctgctcgcg gtggtggtca tcgcggcgat ggacggcgac cgatcgcact tccaccgctt
    17641 cgcccacctg tacctgctcc tgatcctggc cggggtgggc tacggactgg tccgcccgag
    17701 cccaccgttg aaggcgagcc aggccggacg gttcacctgg ctggcggtcc acccgacggt
    17761 gtcgggtgcc atgatgtgcc tggccgcggt cgtcgccgtc ggctacgtcg tcggcggacg
    17821 ctccgaccgg cccggacccc gttggccgcc gttgctctac tgggggatcc tggcggtcgt
    17881 cgccggtgga ggactggcgg gtcacacccg gggggccatc ctcggcggga tctgcggcgc
    17941 cgcggtcgtg ctggtcacga cccgtcgcgg gcgcgccgtt gtcgaggtcg gcctggtggg
    18001 gctcgtcctg gtcgccgggg tggcgatcat ggcgagcgac aaggtgacga cgtacttcga
    18061 acgaggggac acgtcggcgc agctcacgac gctgagccac cggaccgagc tgtggagcgt
    18121 ggcgttcgag tccatcgaga agaagccgtt gttcggcaac gggatcaccg cgtccagggg
    18181 gatcttctac gaggcgaccg gactcggcgg cggtcacaac ggcgccatca acgtgctggt
    18241 cgagctcggt gcggtgggtc tgctggtctg ggccgctctg ctgatcagcc tgctcctcgg
    18301 gatccgtcac ctgccccgca acggaccgag ggacctgcag ctcgaccggg cgatgctgct
    18361 cggggtcatg accttcctgc tggtggactc gatcttctac gagggcatcg gttcggtggc
    18421 caacgtggcg agcacgtggc tcttcatgtg catcggctgg tacgtggtcg cccggcgcga
    18481 cggccgacgc ccgatcgcgg cggccgaggt ggagtccgtt cccgtctcgg ccggcgggtg
    18541 acccgagggg cgctcgacgg cgggagttgc tccggcgatc agcggaaggc gccgaagtgc
    18601 cgggttccgg ccggacgacc gatcaacgcg gcgaccgagg cgggcaactc cggggcggag
    18661 ggatcgagcg cctgccccga cggcgtcgga cgtccggccg catcgacgat cggggcgccc
    18721 gtcaccatct gaccggacgc ttcctgtccg gttgcggtcc ggaacgctcc gaggtcgttg
    18781 aggaccgcgg ggttggtcgc cccccgtgac cacacgacga gccacgtcgg cgagttggcg
    18841 gcgccgcggt ggtaggcgtt cccgggtgag gtgatggcca tctgctcggc gctgaaggtc
    18901 cgggagtagt cctccacgca cagcaggcag ttgccggccg agggggccgc gatcaggttg
    18961 ccctgtacca ggaccggtcc gttgacccag ggcatggtcg gatcggggaa cgggcggcgg
    19021 gggtcgtgac ccggaacgga cgggtcgttg cccctccggc tgtcctggac gatgttcacg
    19081 ggccggttgt tgccgacgaa ggtgttgttg acgagcacga cctcggacgt gtcgttgatc
    19141 ttgatgccgt tgccgccgtt gcccgacacg aggttgtcgg cgaagaccgc cttggccgac
    19201 agctccagcg acgtcccgtg gccgctgttg gcgatcatgt cgcaaccggt cacggtgatg
    19261 tcgtagacgg cctggtcggt ccacaggccc gggccgaggt tgtcgcggaa cacgcagccg
    19321 gtgacggtga ggccccgcgt cctggtcacc ttgaacccgc ctgacaccgg tgcgctgttg
    19381 aagtgctcgg tgttgttggc actcgagacg accccgagca acctcaggcc gtcggcgtag
    19441 ttggcgtgga tgccgagcat cccggagcgg gccacctcga cgttgcgcag ggtcgcgccg
    19501 gtggacacga cgctgacgcc ggtggtggcc atgtcggaga cgacgaggtg ctccagggtc
    19561 accccgggtg cctcgacggt gatcgcgccc atgtcgggga ccgaggggct gtagcggcgg
    19621 aaaccgatgc cgcgcacggt cgagcccgtg gcccggatcg acatcgccct gatcagcgtc
    19681 gatgcgcgga cctcgtggcc ggacgggtcg gtcccgatcc gcagccgcga cccggcgtcg
    19741 tccttgtgga accgaccggg gccgacctcg accgccgagg cgacctgggc ctggggagct
    19801 ccgtcgaccc acacctggtc ggggtgcgcg gccatcggga actgcgggtt cacgaagcgc
    19861 cactggtcag ccgagttgtc ggaggcgccg cgggtgtagg tcggggagtg gtcgaacgag
    19921 acgttccagc cgtcgcgtcg ccatgtggac ccgtcggcga cccacccggt cacggcgtcg
    19981 ctgccgtcga accacacgac ctcgccgggc cagttctgaa tcgtcacggt ccgtcccgac
    20041 gggatggtca ccgactcgtg gtaggtgccg gcccgcacca cgatggtggc gccggtgcgg
    20101 gcgatggcga tggccttccc cagggtccgc aggggagcgg cgaccgaccc attggcggcg
    20161 tcgtcgccgg cgggcgacac gacgatggcc gaggcgggga ccgggtagga ggtcgacccg
    20221 agcgtggtgc cggatccgtg cgcagtggcg ggtgcgagtg caggtgcgga cggtggcacc
    20281 gtcggtcgtg ccggcaccgt ggcggcagcc cgccgggtcg agctgtcggc cagggcggat
    20341 ccgctgtcgt cggactccga tccggcgggt gccccggaac aggcggcagc caacagggtg
    20401 gcgccgatgg tgatcacggc gatctggatg cggtgcttca cggtggagct ctccctcggg
    20461 ccggccgtac cgggtacatc ggcccatgtg cggatcgggt tgagggaaga tccgctcagc
    20521 gcccgtccag gagatcctac gcgtgaatat tgcgatcgtg ttgcgacaac ccacatccgt
    20581 cttctaatta cacggttgtg atcatgacgg gatcgtcatg tggagccgtc gggtgcggct
    20641 gcgtcggtgg ctgctcagag atcgagctcg cgctccagga tgcgtcgcag catcgagctc
    20701 gacgtccgct gcgtgtaggg gacgtaggcc acgtcgacgc cgtgggccgc gaagtccgat
    20761 tcgagcttgt cgcccttgtc ggttcccctc cagtcgtcgc ccttgatgat cacgtcgaag
    20821 tgcagtcgct cccacatctc gagcttgtgg gggacgtcct cggcgaccgc ctcgtcgacg
    20881 aagcggatgg ccccgacgat cgccagacgt tcgtcgatgg gcaccaccgg tcggacaccc
    20941 ttggtgcgct gggacatctc gtcggtcacc acgccggcga tcaggtagtc gcagaactgg
    21001 gtggcgttgc gcagcatgtt gaggtggccg acgtggaaca ggtcatacac cccgggtgcg
    21061 tagccgacgc gacgttcgtt gcgggagggc tggtcggatc cgggcatctc tccgacgata
    21121 tcggcagagg gcgtcgaacc cgacctgacg ggaggtctcc gattatgcgt actgacgtgt
    21181 acacatcgag agatgcaggc ggtggacagc cgggtgtaga actcgggacc gaccgacccg
    21241 aacaaccagg ggggaaccca cagtgaactt cgccttcagc gaggagcagg aagagctccg
    21301 caaggtcgtc cgtgacttcc tgaacgccaa gtccgacgag cccaccgtcc gcgagctcat
    21361 ggacaccgag gccggctacg acgccgcggt gtggacccag atggccgagc agatgggcct
    21421 ccaggggctc gtcatccccg aggagttcgg cgggtccggg tactccttcg tcgagctcat
    21481 cgtcgtgctc gaggagatgg gacgccgcct gctgtgcgcc ccgtacttct ccaccgtcgt
    21541 gctcgccggt cagaccctga tccactccgg tgacgacgcg gccaaggccg cccacctgcc
    21601 ggggatcgcg tcgggcgaga ctgtcgccac cctcgcctac accgaaccga acggtcgctg
    21661 ggacgagtct gcggtgaccg cgacggccac cgcggacggc gacacctgga agatcaacgg
    21721 tgagaagctc tacgtcctcg acggccacac cgcggatctg atcatcaccg cggcccgcac
    21781 cgacgcgggt gtgagcctct tcgcggtggc cggcgacgcc cccggtctta cccgcaccgc
    21841 gctgtcgacc atggaccaga cccgcaagca ggcccgcctc cagtacgcgg acgtgcccgg
    21901 caccctcatc gggaccgacg gcggaggctg ggacgtgctg tccaccgtgc tcgacctcgc
    21961 tgcggtggcc ctcgccgccg aacaggtcgg cggctcccag gaggtcctcg aggccgcggt
    22021 ccagtacgcc aaggaccggg tgcagttcgg acgtcccatc ggctcgttcc aggcgatcaa
    22081 gcacaagtgc gccgacatgc tgctcgaggt cgagtcggcc aagtccgccg
//
LOCUS       DAOQOD010000031        22041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_803_length_22041_cov_12.657145, whole genome shotgun sequence.
ACCESSION   DAOQOD010000031 DAOQOD010000000
VERSION     DAOQOD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_22041_cov_12.657145"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="folP"
                     /locus_tag="PLZ72_04980"
     CDS             complement(<1..791)
                     /gene="folP"
                     /locus_tag="PLZ72_04980"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNA35684.1"
                     /translation="MSPPPPVRMRRPSVFAVLNASPESFSGNRVAADDAADLAEAMVA
                     DGADVLDIGAQSLRTDQPELPVEIELDRLLPVLEAVRGRVGPGVTISVDTYRSRVAEE
                     ALRRGASIVNDPSGLRDPEMADLVASTRGAGPASIVVAFNPGTPKVRRPRDERLDDPV
                     EACLRFVDERIDHLTRAGVDPGQLILDPGPDLYKAPDESVAVLRAVPRLRSELGIGRV
                     LWAVSRKDFVGALTGRLPHERGAGTLGALAAVDLQDRDLIRVHDVA"
     gene            complement(788..1786)
                     /locus_tag="PLZ72_04985"
     CDS             complement(788..1786)
                     /locus_tag="PLZ72_04985"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNA35685.1"
                     /translation="MTDESTPPSRAAGVDPTRRDCAPIRTFHHADRSLDELRAAKGST
                     TVSVCLPARNEAATVGAIVETLVRDTVRTGLVDELIVIDDHSTDDTGPRARHAGARVV
                     DAGSVLPEFGRGHGKGEVLWKSLHVSSGDLVLWCDADLRDFRSHLVTGILGPLLCEPD
                     VEVAKGFYRRAEADGIGGGRVTELVARPLLSLLFPALAGLHQPLSGEYGGRRAVLERL
                     PFVEGYGVEVGLLLHYLRQRGTTGLVQVDLDERHHRHRSLDDLGPQAMAVTQTILRHA
                     TAAGLGHIDGAVPPVPAVAELLRPGRAPHVVDVSERPPMVEVAAYLELVRADRTAR"
     gene            1977..2249
                     /locus_tag="PLZ72_04990"
     CDS             1977..2249
                     /locus_tag="PLZ72_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNA35686.1"
                     /translation="MATIRIPTTLRTLTGGASSVEADGTTVGEVLANLDAAHPGFKDR
                     LFDDQGALRRFVNVFLADDDVRYLQGVDTPVADGAEISIIPAVAGG"
     gene            2280..2618
                     /locus_tag="PLZ72_04995"
     CDS             2280..2618
                     /locus_tag="PLZ72_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35687.1"
                     /translation="MDDDDLFGHEPDGELAEHDPLLPPRLMAVDDVSAAPVDPPAAES
                     VPPRYRRSMGATMLAASMIGLREVLENPRDDRPVVEQHVDEGDVDRPIDVVLDPDDPS
                     ASVVRIRRES"
     gene            complement(2728..4224)
                     /locus_tag="PLZ72_05000"
     CDS             complement(2728..4224)
                     /locus_tag="PLZ72_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35688.1"
                     /translation="MARIVIIGAGVGGLGTALCTARSGHRVTIVERDDTPLPTDPHGA
                     FEWDRHGAPQVRHSHAFLARLRNLLRDRHPDVITALLDAGATELRFLEMAPEGMDLEP
                     EPGDEDLAALACRRTTFEWVLRRMALAEGAVELIHGRSVTSLVTADETVDGIPLVVGV
                     TLSDGTTLDADVVVDAGGRRSDVPALLAPHGVEIPEAEEDTGIIYLSRFFRLLDLDES
                     PPSVVVGADLGYLKYGVFPGDNHTFSITFAVGSRDSEMRRLLLDPDRFLTAAALIPAT
                     APYVDGRSEPITDVEVMGGLINRRRWFLNDGDRPLVAGFHAVGDCHTATNPLYGRGCS
                     LAMVQAQVLADLLSDPAMVADHVARATAYEKAVHAEVTPWYDAAVAQDRFNRSQTPGS
                     PPATPAGADDGVDIAVRPDAGASDPTEVDPSDFLRGVLREGLLPALRSDPVVLRAFLA
                     TFNLLRPPDSLLSDADVIGRVMAIYQTRNERPAEPPLGPTRAAFLAAV"
     gene            4575..6200
                     /gene="groL"
                     /locus_tag="PLZ72_05005"
     CDS             4575..6200
                     /gene="groL"
                     /locus_tag="PLZ72_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNA35689.1"
                     /translation="MAKIIQFDEDARRHLESGMNQLADAVRVTLGPKGRNVVLAKKWG
                     APTITNDGVSIAKEIELEDPYENIGASLVKEVAKKTDDVAGDGTTTATVLAWSMVREG
                     LRNLAAGANPVGIKRGIEAAVDAAVAKIAELAAEVDSKDQIAQVASISAADAEIGTLI
                     SDAIDKVGKDGVITVEESQTFGMGLEFTEGMRFDKGYISPYMVTDAERMEAVLDNPYV
                     LFVSSKITNVRDLVPVLEKVMQSGKPLVIIAEDIEGEALATLVVNKIRGTFNSVAVKA
                     PGFGERRKAMLQDMAILTGGQVISEEVGLKLDSIDLSLLGNADKVVVTKDETTIVNGG
                     GSKEDLEGRIAQIKAEIDNTDSDYDREKLQERLAKLSGGVAVLKVGAATEVELKEKKH
                     RIEDAVSTTKAAIEEGVVAGGGVTLLRAQAAVDELVKKLAGDEATGATLISRALEGPI
                     KQIAENAGLEGGVVVERVRSLGKASEGLNAATGEYEDLVAAGIIDAAKVTRSALQNAA
                     SIAALFLTTEAVVADAPEKAAAGGAPDMGGMDF"
     gene            6450..7373
                     /locus_tag="PLZ72_05010"
     CDS             6450..7373
                     /locus_tag="PLZ72_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA35690.1"
                     /translation="MSDVATVLVTMLVGVGAGILSALLGVGGAVITTPAVRVLGATPI
                     QAVGSTVPAILPSAISGTLRYAREGLVDWSAALGLGISGAVFAVGGALASDLIGGPTL
                     MVFTAVLMLWSGFSVVRGGRRAAAGPGDEGTAAVDLVVPEHGDGSWIDAVEGTEASET
                     ESAFVVEHHEPTGTGPTAGGARGSRHPLPLLVALGATSGFVAGLLGVGGGIVMVPVLT
                     GPLRVPMKSAVASSLVAVAIFSVPALVTHMALGHIDWAYALPLMVGVVPGARIGAHLT
                     IGSSERTVRLLFGTMIVVLALVYGASEVAAL"
     gene            complement(7343..>7612)
                     /locus_tag="PLZ72_05015"
     CDS             complement(7343..>7612)
                     /locus_tag="PLZ72_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA35691.1"
                     /translation="RDDFDARRTGLDHLAFTVATRTDLDRWALRLDQQGVEHSGVLEI
                     PVGAILNLRDPDGIALALFWDDPDRERHRGPATPPGHSAATSDAP"
     assembly_gap    7613..7712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7713..8078)
                     /locus_tag="PLZ72_05020"
     CDS             complement(<7713..8078)
                     /locus_tag="PLZ72_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNA35692.1"
                     /translation="MGPPRPDPALPHLEEMGPDPNLGVLLRNPYLEFSAQLLDHLHTV
                     GYDDLRPAHLVVFQHIDPAGSRITDLARRAQMTKPSISYLVEQLETRGYLERTDDPSD
                     GRARLVHLTTRGWSQITDAL"
     gene            8181..9068
                     /gene="fdhD"
                     /locus_tag="PLZ72_05025"
     CDS             8181..9068
                     /gene="fdhD"
                     /locus_tag="PLZ72_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNA35693.1"
                     /translation="MARSRTESRLVRRFAIAGDERGVASPVDQGRAHQRLLVEEPLEI
                     RVDGTTVATTMRTPGHDFELAVGWCHAEGLLDGVELRGIRYCATGSAVDAGFNVVTVE
                     TSGRGRPVEPRLNPMTSSCGICGTDTVDRLLERLDRLPVGAPLSADVLSSVTEWVAAD
                     QELFRSTGGSHAAAVLSPDGRACVVREDIGRHNAVDKAVGRMVLDGDLPATGSILWIS
                     GRASLEMVQKAWAAGVAALVSVSAPSSLAVRTAERAGMVLIGFARDGRFTVYAGADRV
                     LADSEVGIATRCVSEDPYI"
     gene            9049..11367
                     /locus_tag="PLZ72_05030"
     CDS             9049..11367
                     /locus_tag="PLZ72_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HNA35694.1"
                     /translation="MRTPISDGRVGRALRRIGLHPELWAGLSPNGITQQKPRHYRDMA
                     RIAVDNAHHPKYAWDVLTKGVCDGCALGVAGLHDWTIDGVHLCTTRLELLELNTADPL
                     DPILLSDVDALRSRSGRELRALGRLAHPMRRRRGEAGFRRITWDEALDASAGALAATT
                     PERMAVFLTSRGLTNETYYAAGKAARALGTANIDSAARVCHAPSTLGLKATIGVAAST
                     CSLRDVIESDLVVLWGTNPANNQPVFMKYLDQAKRNGTRVVVVNPFLEPGLERYWVPS
                     SAESAVFGTRICDLHVPVRPGGDIALANAVLKALIARRAVDHSFIDHHTNGWAETVAA
                     LDAQDLDDLLRQAGVDADTFERFVDLYAGADASVLIWSMGITQHTFGVEGVQGIVNLG
                     LARGNVGRNGAGLMPIRGHSGVQGGAEMGAYATALPGGVGIDAGSAAALSQQWGFPVP
                     DHDGLTAPEMVEAAARGELDVVWMSGTNFLEVLPDPVAVAAALDRVPTRIHQDVIVTT
                     QMLVDGDDVILLPVATRYEQEGGGTSTTTERRVAFSPEIPRRVGEARSEWRLFGDIAT
                     RARPERAHAFSWPTNRDLRAEIARVVPAYAGVEFLERTGDAIQWGGRHLCPGGRFPTP
                     DGRGRFTPLEPPTVDVPDGRFLVSTRRGKQFNSIVWADVDPLTGAGRDAVYLDRSDAA
                     ALGLSDGDPVVLRSEVGEMAARVRVTRLPSRTAQVHWPEGNVLLPAGADHREPGSKIP
                     DYQAIVEIVPVRDGPPAQATTRAAPPSGRRAT"
     gene            complement(11319..12077)
                     /locus_tag="PLZ72_05035"
     CDS             complement(11319..12077)
                     /locus_tag="PLZ72_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35695.1"
                     /translation="MRIRSHLRTTALVVLGALVIGTPAACGDSEEDRFSAAADKARTS
                     STAAPERPVGPGTGEPSDRPAGTARPGSTAFPGDTAFPGDRTSTTVATVPAPDALLAA
                     IDAFRTELGTTKAVELTVHFPVGSGPYASLSYQLPDRPTAVDRRDWRDGRVGEASPVK
                     LTGSDDVAARVWDLGSVDWNAVAAAVPQATGLVEAQVGGPLEGSTGVTHLIAADGAPF
                     FDGTVIRVYVDGGTRRTGGYVALRPDGGAARVVA"
     gene            12149..12832
                     /locus_tag="PLZ72_05040"
     CDS             12149..12832
                     /locus_tag="PLZ72_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNA35696.1"
                     /translation="MPEASTPTVGLAVVHLFFRTAPLADRAGIVAALDAAAERGDQAV
                     VVALLGHKADLGVMALSSDQWNLRRLQTDLVAAGLDLVDSYVSLTESSEYAEGIPEEM
                     KQARLYPQLPPEGKPVFCFYPMSKRREVGANWFQLDFDRRKELMYEHGASGRKFRGRV
                     LQVVTGSTGTDDFEWGVTLFCVNPDDVKDVVYTMRYDEASAVYGEFGPFYVGMVADPG
                     TVLGELGID"
     gene            13086..13925
                     /locus_tag="PLZ72_05045"
     CDS             13086..13925
                     /locus_tag="PLZ72_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35697.1"
                     /translation="MALAVVVLVAAVAGAENHGRRRLRDAVAEQLRSSGITGTIDVSV
                     PGGPVPVVLPALFGRGLSELEIRIRDGQVAGLPVARADYVLTDLQGDVSLRTGQVHVR
                     SIGGGDVRIEVAPSALADSIGTELVIRDGRLRAGPDELLVDTEVVGDALVLSGPAESL
                     WGAPIEVPIADGYLLPCTPEVSIRRSVVVLSCRGSKLPGVLRAPLGIDSRGGSDVAPV
                     GSLVPPASTVVAGPGTATTTEDPVTTTIAPTTIAPTETVPVVTVAPPVEPVPAPAAPP
                     APG"
     gene            complement(13934..14218)
                     /locus_tag="PLZ72_05050"
     CDS             complement(13934..14218)
                     /locus_tag="PLZ72_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35698.1"
                     /translation="MRIRATAVFERVIYNCFVTDPSRPERPVLEMDALLRDGDADGPV
                     LLPVPQFMALVGGPAVAEPMLRRLSAQGRVVRHQGVAHLSFPTWQPVADD"
     gene            14269..14739
                     /locus_tag="PLZ72_05055"
     CDS             14269..14739
                     /locus_tag="PLZ72_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35699.1"
                     /translation="MTDWTDEERFAEHGRQLAAAIDAVIEPWVTRCVTETCAAAGIPV
                     DDRVRDAASDAARRCRREVAAEMAALVAADVDAQTVTPLQVLRTSVRFPTEALVDLGV
                     EPPRRDDFDRRAFPEDIYGLGPAGFSDVDPSLRDPGLAWGAAKAHVHRRRHLER"
     gene            complement(14793..14975)
                     /locus_tag="PLZ72_05060"
     CDS             complement(14793..14975)
                     /locus_tag="PLZ72_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35700.1"
                     /translation="MAVRTDCRHYSSRTVTSGEVVQRCRVDAAEPMPFACPEGCLFFE
                     PRSITDAGWSREPGSH"
     gene            15132..15470
                     /locus_tag="PLZ72_05065"
     CDS             15132..15470
                     /locus_tag="PLZ72_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNA35701.1"
                     /translation="MKLVTAVVKPFKLDDVKQALKGVGVVGMTVTEVRGFGRQGGHTE
                     TYRGTEYQVDFLPKVKIEAVVDDADVAAVTDAIVDAARTDKIGDGKVWVTNVDELVRI
                     RTGETGADAV"
     gene            15635..17938
                     /locus_tag="PLZ72_05070"
     CDS             15635..17938
                     /locus_tag="PLZ72_05070"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNA35702.1"
                     /translation="MAIPLHRSSLLDDRSCGGRGFCDRYTQVVEDWLRSLWVDAVAAA
                     GGGDGMALVAVGGQGRGELCPGSDLDLLLLADKGVDAARVADGLWYPIWDERLKLGHA
                     VRSIRDTLALAAEDLETATALLSARHVAGDPALTAELAEKARLAWQRKGRRWLDVLSR
                     AVQARHAAHGEVAFDLEPDLKEGRGGLRDVHALAWARAAGADVDERLLAELFDHHDAL
                     LAVRVELHRAGGRAGDVLTLDDQDDVAARLGDVDADALMTRVAVAGRAIAWSSDESWH
                     EIGTRLATTRFGRASRDRPLDGGLVLRDGRVALADEGSPVADPVAVLRVAVAAARVPT
                     RISPRTLELLSEAPELPDPWPEPARLLLVELLGSGAAAIPVIEVLDRWGLWVRLVPEW
                     EPARSRIQRNALHRFTVDRHLLETASEASRLGAHTRQDLLSMAALLHDLGKGYPGDHS
                     ESGETLATAITTRMGFAAGDVATIAHGVRHHLLLPDVATRRDLDDPATVAMVAEVVGT
                     AERLALLRALSEADGRATGPSAWSDWKAQLVDQLTQRVSARLSGDPIDEVAADPFPSA
                     EQRAMLDAGVLEVRADGDTITVACPDRPGTFHRVAGVLALHGLDVLSASIHSERGMAL
                     DEFRVAVGPSGTVPWDRVGADVARVLDGRLAIQARLDERVRSQLGRRRPGIHQLGHAV
                     RFDDGASADATVVEVVGADSLGLLYRLTRALTDLDVDIRSARIHTLGADVVDTFYVTS
                     AAGGPITDEEHRHEIERALLHALEPVT"
     gene            complement(17970..18851)
                     /locus_tag="PLZ72_05075"
     CDS             complement(17970..18851)
                     /locus_tag="PLZ72_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNA35703.1"
                     /translation="MGNLEWVDGPPDGSRRRSIDRTGPVLIAAFEGWNDAGDAATSAV
                     EHLWEHWGATTVAAIDPEDFYDFTATRPQVRRDPHGRRVLEWPANELGWAEPAGTDGV
                     VLLRGVEPQLRWRTFCETVLGAARDLDCRWILTVGALLADVPHTRPTPVFGTAEDPTL
                     GSRLGLTASDYEGPTGIVGALHSEATAHGVPTASLWAAVPAYAPSVPSPRAALGLVTR
                     TASMLGTDVDTGVLEAAGISYERQLGQLVSEDEATLEYVRLLERRHDAEHLGVDDDSV
                     PNADRLLEEVERFLRDQ"
     gene            18945..19376
                     /locus_tag="PLZ72_05080"
     CDS             18945..19376
                     /locus_tag="PLZ72_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA35704.1"
                     /translation="MRIETCSTVDADLVAAMARLIPQLSRSAPPPDSAALAAIVGSDA
                     CHLLVARDDDGSILGSMTLVVFPIPTGIRAWIEDVVVDDAARGRGVGEALNRHAIALA
                     GSLGATTIDLTSRPSREAANRLYRRIGFEPRETNVYRYQGD"
     gene            19457..21085
                     /locus_tag="PLZ72_05085"
     CDS             19457..21085
                     /locus_tag="PLZ72_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNA35705.1"
                     /translation="MNPQPGTPLDLGRDASGWVDVCALEDIADRHGKAVVVRGTDVAL
                     MRDGDRIHAVGGTCPHRGGQIADGRVVDGEVICPLHLWDFDLRTGISAFNPKDRLPHF
                     DARVRDGRVEIDADSVPAGPGRPDVYLGPWVRRGATDRGMDLVHHLAEGGSPYVESMG
                     STRLQPDSAPMGPHPSLDDLVFLPAQLARLPLLDDEPVATSVRLGTRAARPLDLAVPL
                     VVSHMSFGALSAEAKVALATGAAAAGTAIGSGEGGMLPEERAAAGLYVLEMASGYFGW
                     TEDAVAAADAVEIKIGQGAKPGLGGVLPAAKVTERIAEVRGIEAGVDSHSPSRFPDIG
                     SPAALAERLERIRSINPGIPIGIKIAAGRVDDDVAVAVDVGADYLVVDGFGGGTGAAP
                     VHVRDHVGIPSWVALHRARAWLDDHGHADVQLVVTGGYRTPDEMAKAMALGADAVALA
                     TASMMAIGCQQYRACHRGTCPVGIATQDPVLRSRLDPAVSAERLTRFLLAATEMMTDY
                     ARITGRRSLGELAREDLAALRPDAAALLDIDVRA"
     gene            21193..21975
                     /locus_tag="PLZ72_05090"
     CDS             21193..21975
                     /locus_tag="PLZ72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA35706.1"
                     /translation="MAAPADSARPSLSVTIWSVWSWFAFGLSVIVFIPIILTVYVVTF
                     PFDRTHYWAGYMYRKMPVLHQKLTPMWNFHVRGELPANPRNPYVVVANHESFVDILLI
                     SHLPFEMKWLSKQEMFKIPIAGWLMYVAGDIKLDRGDRDSAKKAMDRCVWYLKHRVSV
                     MVFPEGTRAVDGELGKFKDGAFRLAIEAQVPILPLAVHGTKEALRKHDWRFGRANAAV
                     QVMEPVSTEGLTLEDVDSLKERVRDLISDQLDVMEQDPTLSN"
BASE COUNT         3060 a         7766 c         8008 g         3107 t
ORIGIN      
        1 gccacgtcgt ggacacggat caggtcccgg tcctgcaggt cgaccgcggc cagggcgccg
       61 agggtccccg ccccgcgttc gtggggcaac cggccggtga gcgccccgac gaagtccttg
      121 cgcgacaccg cccacagaac ccgaccgatc ccgagctcgg agcgcaggcg gggcaccgcc
      181 cgcaggaccg cgaccgactc gtcgggcgcc ttgtagaggt ccggacccgg gtcgaggatc
      241 agctggccgg gatcgacccc ggcccgggtc aggtggtcga tgcgctcgtc gacgaaccgg
      301 aggcacgcct cgacggggtc gtcgagtcgc tcgtcccgcg gccggcgcac cttgggggtg
      361 ccggggttga acgccaccac gatcgacgcg gggccggcgc cgcgggtgga ggcgaccagg
      421 tcggccatct cggggtcgcg cagaccggac gggtcgttga cgatcgacgc cccgcgtcgc
      481 agcgcctctt cggcgacgcg actgcggtac gtgtcgaccg agatcgtcac gccggggccg
      541 acccggccgc ggaccgcctc cagcacgggc agcaggcgat cgagctcgat ctcgaccggc
      601 agctcgggct ggtcggtgcg caacgactgc gccccgatgt cgagcacgtc ggcgccgtcg
      661 gcgaccatgg cctcagccag gtctgccgcg tcgtcggcgg ccacgcggtt gccggagaac
      721 gactccgggg acgcgttcag caccgcgaag accgacggcc ggcgcatccg caccggcggg
      781 ggagggctca acgggcggtc cggtcggcgc gcaccagctc gaggtaggcc gccacctcga
      841 ccatcggcgg gcgttcggac acgtccacga cgtggggggc gcggcccggt cgcagcagct
      901 ccgccaccgc cggcaccggg gggacggccc cgtcgatgtg gcccagcccg gcggcggtcg
      961 cgtggcgcag gatcgtctgg gtgaccgcca tggcctgcgg gccgaggtcg tcgaggctgc
     1021 ggtggcggtg gtggcgttcg tccaggtcga cctggaccag ccccgtggtt ccccgctggc
     1081 gcaggtagtg cagcagcaga cccacctcga ccccgtagcc ctcgacgaac ggcaggcgct
     1141 cgagcaccgc ccggcgaccg ccgtactcgc ccgacagcgg ctggtgcagt ccggccaggg
     1201 cggggaacag gagcgacagg aggggccggg cgaccagctc ggtgacccgt ccaccgccga
     1261 ttccgtcggc ctcggctcgt cggtagaagc ccttggccac ctcgacgtcc ggctcgcaca
     1321 ggaggggtcc gaggatgccg gtgaccaggt gggaccggaa gtcgcgcagg tcggcgtcgc
     1381 accagagcac caggtcgccc gaggagacgt gcagcgactt ccacagcacc tcgcccttgc
     1441 cgtgaccgcg gccgaactcg gggagcacgg agccggcgtc gacgacccgc gctccggcgt
     1501 ggcgggcgcg cgggccggtg tcgtcggtcg agtggtcgtc gatcacgatg agctcgtcga
     1561 ccaggccggt gcgcacggtg tcgcgcacca gcgtctcgac gatcgctccg acggtggcgg
     1621 cctcgttccg agccggcagg cacaccgaca ccgtcgtcga tcccttggcg gcccgcagtt
     1681 cgtcgaggga tcgatccgcg tggtggaagg tgcggatcgg cgcgcagtcc cgacgggtcg
     1741 gatcgacgcc ggcggcacgg gacggggggg tggactcgtc ggtcattccg ccgcgcaagc
     1801 taccggtcca cgccggggga atcgtaggcg gcgccccgtg gttgcaaccg ggcgccgggg
     1861 ccgtcgagtt cccctacggc ccgccggtgg tggagaatca ccaccggcga cggtccccgg
     1921 acccgcatcc gcaacgttct gtcccgcccc gacaccaccg ctcccaggag acacccatgg
     1981 cgacgatccg gatcccgacc accctgcgca ccctgaccgg cggcgcatcg tcggtggaag
     2041 cggacggcac caccgtcggc gaggtgctgg ccaacctcga cgcggcccac ccgggtttca
     2101 aggaccggtt gttcgacgac cagggcgcgt tgcgccgctt cgtcaacgtc ttcctggccg
     2161 acgacgacgt ccgctacctc cagggcgtcg acaccccggt ggccgacggc gccgagatct
     2221 cgatcatccc ggcggtcgcc gggggctgag ccgatccggg ccgtcgtagg ctggccgccg
     2281 tggacgacga cgacctcttc ggccatgagc ccgacggcga gctcgccgag cacgatccgc
     2341 tgctgccgcc gcggctgatg gccgtcgacg acgtctccgc tgctccggtc gacccgccgg
     2401 ccgcggagag cgtccctccc cggtaccgcc ggtcgatggg cgccaccatg ttggcggcgt
     2461 cgatgatcgg actgcgagag gtcctcgaga accccaggga tgatcgcccg gtggtcgagc
     2521 agcacgtgga cgagggcgac gtcgaccgcc cgatcgacgt cgtgttggac cccgacgacc
     2581 cgtcggccag cgtggttcgc atccgtcgag agagttgagt cagccgtccg gcggcgggcc
     2641 ggtgggttcc gcggtctctc ggtcagacgg tctttttggg gcaggcggtc cttgggccag
     2701 gcggtccttg ggtcaggcgg tccgcggtca gaccgcggcc aggaaggcgg cccgggtcgg
     2761 ccccagcggc ggctccgctg gccgctcgtt ccgggtctgg tagatcgcca tcacccgtcc
     2821 gatcacgtcc gcgtcgctga gcagcgagtc cggcggacgc aacaggttga acgtggccag
     2881 gaacgcccgc aggacgaccg gatcgctgcg cagggccggc agcagcccct cgcgcagcac
     2941 ccctcggagg aagtccgacg ggtcgacctc ggtcgggtcg gacgcccctg catccgggcg
     3001 cacagcgatg tccacgccgt cgtcggctcc ggccggtgtc gccggcgggg aaccgggggt
     3061 ctgggagcgg ttgaagcggt cctgcgccac cgcagcgtcg taccaggggg tgacctcggc
     3121 gtgcaccgcc ttctcgtacg cggtcgcccg ggcgacgtgg tcggccacca tcgctgggtc
     3181 cgacaggagg tcggccagga cctgggcctg gaccatggcc aggctgcacc cgcgtccgta
     3241 cagcgggttc gtggcggtgt ggcaatcacc gacggcgtgg aacccggcca ccagcgggcg
     3301 atcgccgtcg ttgaggaacc agcggcgacg gttgatcagc ccgcccatca cctcgacgtc
     3361 ggtgatcggc tcggaacgac cgtcgacgta gggggcggtg gcggggatga gagcggcggc
     3421 ggtgaggaag cggtcggggt ccagcagcag ccgacgcatc tcggagtcgc ggctgccgac
     3481 ggcgaacgtg atcgagaagg tgtggttgtc gccggggaac acgccgtact tgaggtagcc
     3541 caggtccgct ccgaccacga ccgacggagg tgactcgtcg aggtcgagca gccggaagaa
     3601 gcgggacagg tagatgatcc cggtgtcctc ctcggcctcg gggatctcca cgccgtgcgg
     3661 ggcgagaagg gcgggcacgt cgctgcgtcg gccgccggcg tcgacgacca cgtccgcgtc
     3721 gagggtggtt ccgtccgaca gggtcacgcc gacgaccaga ggtatcccgt cgacggtctc
     3781 gtccgcggtc accagcgagg tgacggagcg gccgtggatc agctcgacgg ctccctcggc
     3841 cagcgccatc cgccgcagca cccactcgaa ggtggtgcga cgacaggcca gggcggccag
     3901 gtcctcgtcg ccgggttcgg gctccagatc catcccctcg ggggccatct cgaggaagcg
     3961 caactcggtc gctccggcgt cgagcagtgc ggtgatgacg tcggggtggc ggtcgcgcag
     4021 caggttgcgg agccgggcca ggaacgcgtg ggagtggcgg acctggggcg caccgtgccg
     4081 gtcccactcg aaggcgccgt gcgggtcggt gggaaggggg gtgtcgtcgc gttcgacgat
     4141 cgtcacgcgg tgcccggacc gggcggtgca caacgcggtc cccaggccgc cgacgccggc
     4201 gccgatgatc acgatgcgtg ccacgtcgtc ctccctctcg acttcctcgt gggccgtggg
     4261 cggcgcggtc cgcccccgac acccgtgctg gctccccggg tgggtcgggg tggcgttccc
     4321 tgccggtgtc gtccggtcga gttgaccgaa cggtcaagtt cctggagggc acggtaacag
     4381 ggtccgtcgc ctgagaagcc ggcccgggtg ggcacccggc tgttagcact caccatccct
     4441 gagtgctaag aatgtctggc actctcatgc cgcgagtgcc aacccgggac cgggaggtcg
     4501 gcgtcagccc gaccgatccg cctccgggac cgaaccgccc agaagcaacc gccgcagttc
     4561 caacggagga ctccatggcc aagatcatcc agttcgacga ggacgcacgc cgccacctcg
     4621 agtcgggcat gaaccagctc gccgacgcgg tccgcgtcac gctcggcccc aagggtcgca
     4681 acgtcgtgct cgccaagaag tggggcgccc cgacgatcac caacgacggc gtctccatcg
     4741 ccaaggagat cgagctcgag gacccgtacg agaacatcgg cgcctcgctg gtcaaggagg
     4801 tcgccaagaa gaccgacgac gtcgccggtg acggcaccac caccgccacc gtgctcgcct
     4861 ggtcgatggt gcgcgagggg ctgcgcaacc tcgccgccgg tgccaacccg gtcggcatca
     4921 agcggggcat cgaggccgcc gtcgacgccg cggtcgccaa gatcgccgag ctcgccgcag
     4981 aggtcgactc caaggaccag atcgcccagg tcgcgtccat ctccgccgcg gacgccgaga
     5041 tcggaacgct gatctccgac gccatcgaca aggtcggcaa ggacggcgtc atcaccgtcg
     5101 aggagagcca gaccttcggc atgggcctcg agttcaccga gggcatgcgc ttcgacaagg
     5161 gctacatctc gccgtacatg gtgaccgacg ccgagcgcat ggaagcggtg ctcgacaacc
     5221 cgtacgtgct gttcgtgtcg tccaagatca ccaacgtccg cgacctggtg cccgtgctgg
     5281 agaaggtcat gcagtcgggc aagccgctgg tgatcatcgc cgaggacatc gagggcgagg
     5341 cgctggccac cctggtcgtc aacaagatcc gcggcacctt caactcggtc gcggtcaagg
     5401 ccccgggctt cggtgagcgt cgcaaggcga tgctgcagga catggccatc ctcaccggcg
     5461 gccaggtcat ctccgaggag gtcggcctca agctcgactc catcgacctc agcctgctcg
     5521 gcaacgccga caaggtcgtc gtcaccaagg acgagacgac catcgtgaac ggtggcggct
     5581 ccaaggagga cctcgagggc cgcatcgccc agatcaaggc cgagatcgac aacaccgact
     5641 ccgactacga ccgcgagaag ctccaggagc gcctcgccaa gctgtcgggc ggcgtggccg
     5701 tcctcaaggt cggcgccgcc accgaggtgg agctcaagga gaagaagcac cgcatcgagg
     5761 acgccgtgtc gaccaccaag gccgccatcg aggagggtgt cgtcgccggc ggtggcgtga
     5821 ccctgctgcg ggcccaggcc gccgtcgacg agctggtcaa gaagctggcg ggcgacgagg
     5881 ccaccggtgc caccctgatc tcccgtgcgc tcgagggccc gatcaagcag atcgccgaga
     5941 acgccgggct cgagggcggc gtcgtggtcg agcgggtgcg ctcgctcggc aaggcctccg
     6001 aggggctcaa cgccgccacc ggcgagtacg aggacctggt cgccgccggc atcatcgacg
     6061 ccgccaaggt cacccgctcc gctctgcaga acgcggcgtc gatcgctgcg ctgttcctga
     6121 ccaccgaggc cgtcgtggcc gacgccccgg agaaggctgc ggccggcggc gcccccgaca
     6181 tgggcgggat ggacttctga tcccgatctg acccgggctt cgcccggagc tcgctgggat
     6241 ggacgagggc ggccctccgg ggtcgccctc gtcgcgtccg gcgaccgacc tcctcccgcc
     6301 ccgaacttgt aggtggcggt cgctgtggtg gcgtgtgggc tacaagttgg tcgggctccg
     6361 ccgaacttgt tgctggcggt cgctgtggtg gcgtgtgggc tacaagttcg gctcggcggg
     6421 agtgctcacg tagcgtcggt ccggtggaca tgtccgacgt cgccaccgtg ctggtcacga
     6481 tgctcgtcgg cgtcggggcc ggaatcctgt cggcgctgct gggggtcggc ggtgcggtca
     6541 tcaccacgcc ggcggtgcgg gtgctcggcg cgacgccgat ccaggccgtc ggctctaccg
     6601 ttcccgccat cctcccgagc gcgatctcgg gcaccctgcg ctacgcccgc gagggcctgg
     6661 tcgactggtc cgccgcgctc ggcctcggca tctccggggc ggtcttcgcc gtcgggggtg
     6721 cgctggcatc ggacctgatc ggtggaccga ccctcatggt gttcaccgct gtgctgatgc
     6781 tgtggtccgg gttctcggtc gttcggggcg gacgtcgggc ggcggcgggg ccgggcgatg
     6841 agggcaccgc ggcggtcgac ctcgtggtcc ccgaacacgg tgacggctcg tggatcgacg
     6901 cggtcgaggg gaccgaggcg tccgaaaccg agagcgcgtt cgtcgtcgag caccacgagc
     6961 cgaccgggac gggcccgacc gcaggcgggg ccagaggctc gcggcacccc ttgccgctgc
     7021 tggtggctct cggggcgacg tcggggttcg tcgccggtct gctcggcgtc ggcggaggca
     7081 tcgtgatggt gccggtgctc accgggccgt tgcgggtccc gatgaagtcg gcggtggcct
     7141 cgagcctggt ggcggtggcg atcttctcgg tgccggccct ggtcacccac atggctctgg
     7201 gccacatcga ctgggcgtat gccctgccac tcatggtggg ggtcgtgccc ggcgctcgca
     7261 tcggagcaca cctcaccatc gggtcctcgg agcgcacggt gagattgctg ttcggaacga
     7321 tgatcgtggt gctggccctg gtctacggcg catcggaggt ggccgcgctg tgacccggcg
     7381 gcgtcgccgg tcctcggtgg cgttcccggt cgggatcgtc ccagaacagg gcgagcgcga
     7441 ttccgtcagg atcccggagg ttgaggatcg ctccgacggg aatctccagc acgcctgagt
     7501 gctcgactcc ctgctggtcg aggcggaggg cccaccggtc gaggtcggtt cgggtcgcga
     7561 cggtgaaggc gaggtggtcc agcccggtcc ggcgggcgtc gaagtcatcc cgnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagcgcat cggtgatctg gctccacccg
     7741 cgcgttgtga ggtggacgag cctcgcccgg ccgtcgctgg gatcatcggt tcgttccaga
     7801 tagccgcgtg tctccagttg ttcgacgagg taggagatcg acggcttggt catctgcgcc
     7861 cgtcgggcga ggtcggtgat cctcgagccg gccgggtcga tgtgttggaa gacgaccagg
     7921 tgcgcgggcc ggaggtcgtc gtagcccacg gtgtggaggt gatccaggag ctgtgcggag
     7981 aactccaggt aggggttcct caggaggacc ccgaggttgg ggtcgggtcc catctcctcg
     8041 aggtgtggga gcgctgggtc ggggcgaggt gggcccatga agcggagtat cgcacacgat
     8101 tggaagaatg tcttacttac gtcagcaggt cgcgtacggt tccgcggttg aggggatcct
     8161 ggcccccgta ggctggcgca gtggcccgat cccgtaccga gagccggctc gtcaggcgct
     8221 tcgcgatcgc tggcgacgag cgtggggtcg ccagccccgt cgaccagggg cgggcgcacc
     8281 aacgactcct ggtcgaggag ccgctggaga tccgtgtcga cgggacgacc gtcgccacca
     8341 ccatgcgcac gccgggccac gacttcgagc tggcggtcgg gtggtgtcac gccgaaggcc
     8401 tcctcgacgg tgtcgagctc cgggggatcc gctactgcgc caccggatct gcggtcgacg
     8461 ccggcttcaa cgtggtgacc gtggagacct cggggcgtgg ccggcccgtc gaaccccggc
     8521 tcaacccgat gacgtcgtcg tgcgggatct gcggaaccga caccgtcgat cgactgctcg
     8581 agcgactgga ccgcctcccg gtcggcgctc cactgtcggc cgatgtgctc tcgtcggtca
     8641 ccgagtgggt ggcggcagac caggagctgt tcaggtccac cggcgggagc cacgccgcag
     8701 cggttctctc acccgatggc cgggcgtgtg tggtgcgcga ggacatcggg cgtcacaacg
     8761 cggtcgacaa ggccgtcggg cggatggttc tcgacggcga cctcccggcc accggatcga
     8821 tcctctggat ctcgggccgg gccagcctcg agatggtgca gaaggcgtgg gccgccggcg
     8881 ttgcggccct ggtgtcggtc agcgctccga gctcgttggc ggttcggacc gcggagcggg
     8941 cggggatggt gctgatcggg ttcgcgcggg acggccggtt cacggtgtac gcgggcgccg
     9001 atcgggttct ggcggattct gaggtcggga ttgcgacaag gtgtgtgagt gaggacccct
     9061 atatctgacg ggagggtggg gcgggcgctg cggcggatcg gcctgcaccc cgagttgtgg
     9121 gccgggctca gccccaacgg catcacccag cagaagccgc ggcactaccg cgacatggcc
     9181 agaatcgccg tcgacaacgc acaccacccg aagtacgcgt gggacgtcct caccaagggc
     9241 gtgtgcgacg ggtgcgccct gggcgtggcc ggcctgcacg actggaccat cgacggcgtc
     9301 cacctgtgca ccacgcgcct ggagctgctc gagctcaaca ccgcggaccc actcgacccg
     9361 atcctgctca gcgacgtcga cgcgctccgg tcgcgctccg gcagggagct gcgggccctc
     9421 ggacgcctgg cgcaccccat gcgtcgccgt cgtggcgagg ccggcttccg tcgcatcacc
     9481 tgggacgagg ccctcgacgc gtcggccggg gcgctggccg ccacgacccc ggagcgtatg
     9541 gcggtcttcc tcacctcccg cgggctcacc aacgagacct actacgcggc cggcaaggcc
     9601 gcccgcgccc tcgggaccgc caacatcgac tccgcggccc gggtgtgcca cgcgccgtcc
     9661 accctcgggc tgaaggccac gatcggcgtc gccgcctcga cctgctcgtt gcgcgacgtc
     9721 atcgagagcg atctggtcgt gctgtggggc accaacccgg ccaacaacca gccggtgttc
     9781 atgaagtacc tcgaccaggc caagcgcaac ggcacccggg tcgtggtcgt caatccgttc
     9841 ctggaaccgg gactcgagcg gtactgggtc ccgagctcgg cggagtccgc ggtgttcggg
     9901 acacgcatct gcgacctgca cgttccggtg cggcccggtg gcgacatcgc gctggccaac
     9961 gcggtgctca aggcgctcat cgcccgccgg gcggtcgacc actcgttcat cgatcaccac
    10021 accaacggct gggccgagac ggtggccgcc ctcgacgccc aggacctcga cgacctgctg
    10081 aggcaggccg gcgtcgacgc ggacaccttc gagcggttcg tcgacctcta cgccggagcg
    10141 gacgcgtcgg tcctgatctg gtcgatgggg atcacccagc acacgttcgg agtcgagggg
    10201 gtccagggga tcgtcaacct gggcctggcc cgcggcaacg tcgggcgcaa cggcgccggg
    10261 ctcatgccga tccggggtca ttcgggagtg cagggcggcg ccgagatggg ggcctacgcc
    10321 accgcgctgc ccggcggcgt cggcatcgac gccgggtcag ctgcggcgct gtcgcagcag
    10381 tggggcttcc cggtgccgga ccacgacggc ctgaccgccc ccgagatggt cgaggcggcg
    10441 gcccggggcg agctcgacgt cgtgtggatg tcgggcacga acttcctgga ggtgctgccc
    10501 gacccggtcg cggtggccgc cgcgctcgac cgcgtcccga cccgcatcca ccaggacgtg
    10561 atcgtcacca cccagatgct cgtcgacggc gacgacgtga tcctgctccc cgtcgccacc
    10621 cgctacgagc aggagggcgg cggcacctcg acgaccaccg aacgtcgcgt cgcgttctcg
    10681 cccgagatcc cccgccgggt cggcgaggcc cgcagcgagt ggcggctgtt cggcgacatc
    10741 gcgacccggg cccgccccga gcgggcccac gcgttctcct ggccgaccaa ccgggacctg
    10801 cgcgccgaga tcgcccgggt ggtccccgcg tacgccggcg tcgagttcct ggagcggacc
    10861 ggcgacgcga tccagtgggg gggacggcac ctctgtccgg gaggtcgctt cccgacaccg
    10921 gacgggcgcg gccgcttcac gccgctggag ccgccgaccg tcgacgtccc cgacgggcgg
    10981 ttcctggtgt cgacccggcg gggcaagcag ttcaactcca tcgtgtgggc cgacgtcgac
    11041 cctctgaccg gggcgggacg agacgccgtg tacctcgacc gctccgacgc cgccgcgttg
    11101 ggcctgtccg acggcgatcc cgtcgtgctg cgctccgagg tcggcgaaat ggccgcccgg
    11161 gttcgcgtga cccgcctgcc gtcacgcacg gcccaggtcc actggcccga gggcaacgtg
    11221 ctgctgcccg ccggggccga tcaccgggaa cccggctcga agatcccgga ctaccaggcg
    11281 atcgtggaga tcgtcccggt gcgggacggc cccccggctc aggccaccac ccgagccgcg
    11341 cctccgtcgg gacgcagggc gacgtagccg ccggtgcggc gggtgccgcc gtcgacgtac
    11401 acgcggatca cggtcccgtc gaagaacggc gccccgtccg ccgcgatcag gtgggtgacg
    11461 ccggtcgatc cctcgagcgg tccaccgacc tgcgcctcga ccaggcccgt tgcctgggga
    11521 acggcggcag caaccgcgtt ccagtcgacc gagccgaggt cccacacccg cgccgcgacg
    11581 tcgtcggagc cggtcaactt caccggtgag gcctcaccga cccgtccgtc gcgccagtcc
    11641 cgtcggtcga ccgcggtggg ccggtcgggc agctggtagc tcaggctggc gtaggggccg
    11701 ctgccgacgg ggaagtgcac cgtgagctcc acggccttcg tggtcccgag ctcggtccgg
    11761 aacgcgtcga tcgccgcgag cagcgcgtcc ggggcgggca ccgtggcgac cgtggtcgag
    11821 gtcctgtctc ccggaaacgc ggtgtcgccg gggaaggcgg tactcccggg acgcgcggtg
    11881 ccagccggcc gatcggatgg ttcgccggtc cccggcccga ccggacgctc gggggcggcg
    11941 gtgctcgacg tgcgggcctt gtcggccgcc gcggagaagc ggtcttcctc ggagtcgccg
    12001 catgcggccg gagtcccgat cacgagtgcg ccgaggacga cgagagccgt cgttcggagg
    12061 tgcgacctga tcctcatccc cgcagtgtgc ccgaaccgct gctgtgtccg atgcgaccgg
    12121 gatcgagtcg gtccctacac tcgcgcccat gcccgaggca tccaccccga ccgtcggcct
    12181 cgccgtggtc cacctcttct tccgcacggc accgctcgcg gaccgcgccg ggatcgtcgc
    12241 cgctctcgac gccgccgccg aacggggcga ccaggcggtg gtcgtcgcgc tgctcggcca
    12301 caaggcggac ctcggtgtca tggcgctgtc gtcggaccag tggaacctcc gccgactcca
    12361 gaccgacctg gtcgctgccg gcctggacct ggtcgacagc tacgtgtcgc tgaccgagag
    12421 ctcggagtac gccgagggga tccccgagga gatgaagcag gcccggctgt acccgcagct
    12481 gccgcccgag gggaagccgg tgttctgctt ctaccccatg tcgaagcggc gggaggtcgg
    12541 cgccaactgg ttccagctcg acttcgatcg acgcaaggag ctcatgtacg agcacggtgc
    12601 ctcgggtcgc aagttccgcg gtcgggtcct gcaggtcgtc accggttcca ccggcaccga
    12661 cgacttcgag tggggtgtga ccctgttctg cgtgaacccc gacgacgtga aggacgtcgt
    12721 gtacaccatg cgctacgacg aggcctcggc ggtgtacggc gagttcgggc cgttctacgt
    12781 gggcatggtc gcggaccccg ggaccgtcct cggtgagctc gggatcgact gagggtccgg
    12841 ccgcgggtgc cggactcgcc gccccggccg agcgtcgcgg ggcaccgccc gacgggcgtg
    12901 ccgtcctcga cggaccgctc cccgcggaac ccgcggctcc cgaaccgacc ggggtcggcc
    12961 cggccgggag gagggcggcg ccggaacggg cgtggagtcg tccggtctgg gcgtcccgga
    13021 ccgcggacga gatcgacgga gaccgcaccg gatcgccgtc gcgccgctgg gtccgctgga
    13081 gcgccgtggc cctcgccgtc gtggtgctgg tcgcggcggt cgcgggcgcc gagaaccatg
    13141 gtcgccggcg cctgcgcgac gcggtcgccg agcagctccg ctccagcggc atcaccggga
    13201 ccatcgacgt ctccgttccg ggaggaccgg tgccggtggt cctgcccgcc ctcttcggac
    13261 gtggtctgtc ggagctggag atccggatcc gcgacgggca ggtggccggg ctgccggtcg
    13321 ctcgggccga ctacgtcctg accgacctgc aaggggacgt gtcgctgcgg accggtcagg
    13381 tccacgtgcg ttcgatcggc gggggtgacg tccgcatcga agtcgctccg agcgcgttgg
    13441 ccgacagcat cggcaccgag ctggtcatcc gcgacggccg gctccgagcc gggcccgacg
    13501 agctgctggt cgacaccgag gtcgtcggcg atgcgctcgt cctgtccggg ccggccgagt
    13561 cgctctgggg cgcgccgatc gaggtgccga tcgcggacgg ctacctcctg ccctgcaccc
    13621 cggaggtgag catccggcgt tcggtggtcg tgctgtcctg ccggggatcg aagctcccgg
    13681 gtgtcctgcg cgcacccctg ggcatcgaca gccgcggcgg cagcgacgtc gccccggtcg
    13741 gctcgctcgt gccccccgcc tcgaccgtcg tcgccggtcc gggaacagcg accaccaccg
    13801 aggaccctgt cacgaccacg atcgccccga ccacgatcgc cccgactgag acggtcccgg
    13861 tggtcaccgt cgctccgccg gtcgagccgg ttcccgcgcc ggccgcccca cccgcaccgg
    13921 gctgaacgcc gggtcagtcg tcggcgacgg gctgccaggt cgggaacgac aagtgggcca
    13981 caccctggtg gcggaccacg cgaccctggg ccgacagccg acgcagcatc ggctcggcca
    14041 ccgcgggccc gccgacgagc gccatgaact gcgggaccgg cagcagcacc ggaccatcgg
    14101 cgtcgccgtc ccgcagcagg gcgtccatct cgagcaccgg acgctcgggc cgcgacgggt
    14161 ccgtcacgaa gcagttgtat atcacccgct cgaacaccgc cgtcgcacgg atgcgcatcc
    14221 accgtttgta cccgaaccgg gacctgcagg cggaccagac tggggtcggt gaccgattgg
    14281 accgacgagg agcgcttcgc cgagcacggg cgacagctgg ccgcagccat cgacgcggta
    14341 atcgagccat gggtgacgcg ttgcgtgacc gagacctgtg ccgcggccgg gatccccgtc
    14401 gacgaccggg tccgggacgc tgcgtctgac gcggcacgac ggtgccgccg agaggtcgcc
    14461 gcggagatgg ccgccctggt cgctgccgac gtcgatgccc agaccgtcac cccgctgcag
    14521 gtgctgcgga cctcggtccg attccccacc gaggccctgg tcgacctcgg cgtcgagccc
    14581 ccgcgacgcg acgacttcga tcggagagcc ttccccgagg acatctacgg actgggaccg
    14641 gccgggttca gcgacgtgga tccgtcgctg cgggacccgg gactggcgtg gggcgcggcc
    14701 aaggcccacg tgcaccggcg acgtcacctc gagaggtgac gatgccgaca ggccggcggg
    14761 gcgggccccc ggaacccgag acgggaccgc tgtcagtggg agccgggctc gcggctccag
    14821 ccggcgtcgg tgatcgaacg cggctcgaag aacaggcacc cctccgggca ggcgaacggc
    14881 atcggctccg ccgcgtcgac gcggcatcgc tggacgacct ccccggacgt cacggtccga
    14941 ctcgagtagt ggcggcagtc ggtgcgaacg gccatggcac gatcctacgg gcgacccgac
    15001 acgtaaccga gcctcccgcc gccggaaaca ctgccgaaac aaggggtccg tagcttcatg
    15061 gccaccccgg tggaacgagc gccggaacct gagcaccgtg tcgccgaccg gaccgagaag
    15121 ggaacctacg catgaagctg gtcaccgccg tcgtcaagcc gttcaagctc gacgacgtca
    15181 agcaggcact gaagggggtc ggcgtggtcg gcatgaccgt gaccgaggtc cggggcttcg
    15241 gccgccaggg cggccacacc gagacctacc ggggcaccga gtaccaggtc gacttcctgc
    15301 ccaaggtcaa gatcgaagcc gtcgtcgacg acgccgacgt ggcggcggtg accgacgcca
    15361 tcgtcgacgc cgccaggacc gacaagatcg gtgacggcaa ggtctgggtg acgaacgtgg
    15421 acgagttggt ccggatccgc accggcgaga ccggcgcgga cgccgtgtga gctgaccgga
    15481 cccaggatcc gggggcgacc gtcctacgcg cggtacacgc cgaactgtgc cgacggcggt
    15541 cgctcccgac gcgcgctcgg gcgggtggga cgggccgctc gctcgtcgcc gccggatccg
    15601 agagccgcac ccgcatgatc acggctgact gaacatggcc atcccgctgc accggagctc
    15661 cctgctcgac gaccgctcct gtggtgggcg cgggttctgc gatcgctaca cgcaggtggt
    15721 cgaggactgg ctgcgctccc tgtgggtcga tgccgtggcc gccgccggtg gcggcgacgg
    15781 catggcgctc gtcgcggtcg gtggccaggg gcgaggtgag ctctgccccg gttccgacct
    15841 cgacctgctc cttctcgccg acaagggcgt cgatgccgcc cgggtcgccg acggcctgtg
    15901 gtacccgatc tgggacgaac gactgaagct cggacacgcg gtgcgcagca tccgtgacac
    15961 cctggcgctc gcagcggagg acctggagac cgcgacggct ctgctgtcgg ctcgccacgt
    16021 ggccggtgac cccgcgttga ccgccgagct ggccgagaag gcccgactgg cgtggcagcg
    16081 caagggtcgc cgctggctcg atgtcctgtc ccgggcggtg caggcccgcc acgccgcgca
    16141 cggagaggtc gccttcgacc tggagcccga tctcaaggag gggaggggag gactgcgcga
    16201 cgtccacgct ctggcctggg cgcgcgccgc gggagccgac gtcgacgaac ggctgctcgc
    16261 cgagctgttc gaccaccacg acgccctcct ggctgttcgg gtggagctgc accgagccgg
    16321 tggtcgggcc ggcgacgtgc tgaccctcga cgaccaggac gacgtggcgg cccggctcgg
    16381 cgacgtggac gcggatgccc tgatgacccg ggtggcggtc gccggtcggg cgatcgcgtg
    16441 gtcgagcgac gagagctggc acgagatcgg cacccggctc gccacgaccc ggttcggtcg
    16501 cgccagtcgc gaccggccgc tcgacggtgg cctggtcctg cgcgacgggc gtgtcgccct
    16561 ggccgacgag gggtccccgg tggccgaccc cgtggccgtc ctgcgggtcg cggtcgcggc
    16621 ggcgagggtg ccgacgcgga tatcgccccg gaccctcgaa ctgctgagcg aagcccccga
    16681 gctgcccgac ccgtggccgg agccggcccg tctcctgctc gtcgagctcc tggggtcggg
    16741 cgctgcggcg atcccggtca tcgaggtgct cgaccgctgg gggctctggg tgcggctggt
    16801 ccccgagtgg gagccggcgc ggtcgcgcat ccagcgcaac gccctgcacc gcttcaccgt
    16861 cgaccgtcac ctgctggaga cggcgtcgga ggcgtcacgg ctgggggccc acacgcggca
    16921 ggacctcctg tcgatggcgg cgttgctcca cgacctggga aagggctatc ccggcgacca
    16981 cagcgaatcg ggcgagaccc tggcgacggc gatcacgacc cggatgggct tcgccgccgg
    17041 cgatgtggcg accatcgccc acggggttcg tcaccacctg ctgctgccgg acgtggcgac
    17101 ccgacgggac ctggacgacc cggcgacggt ggcgatggtc gccgaggtgg tcggcaccgc
    17161 cgagcggctg gcgctgttgc gggcgttgtc cgaagccgac ggtcgggcca cgggtccctc
    17221 cgcgtggagc gactggaagg cgcagctggt cgaccagctg acccagcggg tgtccgcccg
    17281 gctcagcggt gacccgatcg acgaggtggc cgcggatccg ttcccgtcgg ccgagcagcg
    17341 ggcgatgctg gacgccggcg tgctcgaggt ccgggccgat ggcgacacca tcaccgtggc
    17401 ctgtcccgac cgcccgggca ccttccaccg cgtcgccggg gtgctggccc tgcacggcct
    17461 cgatgtcctc agcgcctcga tccactccga gagggggatg gccctcgacg agttccgcgt
    17521 cgcggtcggc ccgagcggca ccgttccgtg ggatcgggtg ggcgcggacg tggccagggt
    17581 gctggacggg cggctggcga tccaggctcg actcgacgag cgggtccgca gccaactcgg
    17641 tcgccgacgg ccgggcatcc accagctcgg ccacgccgtg cgattcgacg acggggcctc
    17701 ggccgacgcc acagtcgtcg aggtggtcgg tgccgactcg ctgggtctcc tgtaccggct
    17761 gacccgggcg ttgaccgacc tcgacgtcga catccgcagc gccaggatcc acaccctggg
    17821 tgcggacgtg gtcgacacgt tctacgtgac ctccgctgcc ggcggcccga tcaccgacga
    17881 ggagcatcgc cacgagatcg agagggcgct gctgcacgcc ctcgagccgg tgacctgagc
    17941 ccggaggggc cgtgctcatc ccgacggact cactggtcgc gcaggaagcg ctcgacctcc
    18001 tcgagcagcc ggtcggcgtt cgggaccgag tcgtcgtcga ctccgaggtg ctcggcgtcg
    18061 tggcggcgtt cgagcaggcg cacgtactcg agcgtcgcct cgtcctcgga cacgagctgg
    18121 ccgagctggc gctcgtagct gatcccggcg gcctcgagca cgccggtgtc gacgtcggtg
    18181 ccgagcatcg acgccgtccg ggtcaccagg ccgagcgccg cccggggcga cgggaccgac
    18241 ggggcatagg ccggtaccgc ggcccacaac gacgccgtgg gcaccccgtg ggcggtcgct
    18301 tcgctgtgca gcgctcccac gatgccggtc ggcccctcgt agtccgacgc ggtgaggccc
    18361 agtcgtgacc cgagggtcgg gtcctccgca gtgccgaaca ccggcgtggg acgggtgtgg
    18421 ggaacgtccg cgagcagcgc gcccacggtc aggatccagc ggcagtccag atcgcgagcg
    18481 gccccgagca cggtctcgca gaaggtccgc caccgcagtt gaggctcgac tccgcgcagc
    18541 aggaccacgc cgtcggtccc cgccggttcc gcccagccca gctcgttggc cggccactcg
    18601 aggacccggc ggccgtgggg gtcccggcgg acctgcggtc gggtcgcggt gaagtcgtag
    18661 aagtcctcgg ggtcgatcgc cgcgaccgtg gtggcgcccc agtgctccca gaggtgctcg
    18721 acggcgctgg tggccgcgtc accggcgtcg ttccagccct cgaaggccgc gatcagcacg
    18781 gggccggtgc ggtcgatcga tcgccgtcga gagccgtccg gcggcccgtc gacccactcc
    18841 aggttcccca tcggttcacc atatcggtga gccccgaccg ctccaccggg gtcgagcggt
    18901 tccggcggat cgctcccggt cccggcgggg tagcgtccac gctcatgcgg atcgagacct
    18961 gcagcaccgt cgacgccgac ctggtggcgg cgatggcccg cctgatcccc cagctgtcgc
    19021 ggtcggcccc tccgccggac agcgcggccc tcgccgcgat cgtcggatcc gacgcatgcc
    19081 acctgttggt ggcccgcgac gacgacgggt cgatcctggg gtcgatgacg ctggtggtgt
    19141 tccccatccc gaccgggatc cgcgcctgga tcgaagacgt cgtggtcgac gacgccgccc
    19201 ggggccgcgg cgtgggcgag gcgctcaacc gtcacgccat cgcgctggcc gggtcgctcg
    19261 gggcgacgac gatcgacctg acgtcgcgcc ccagccgcga ggcggccaac cgcctctacc
    19321 gacggatcgg cttcgaaccc cgcgagacca acgtctaccg gtaccaggga gactgatcga
    19381 cccgggtccg cccacacgtg accgtcccgt cagccacgtc agccgcgagc gaccccccga
    19441 caccggaggc aacgaggtga acccgcagcc cggaaccccc ctcgacctcg gccgtgacgc
    19501 ctcgggctgg gtcgacgtgt gcgccctcga ggacatcgcg gaccggcacg gcaaggccgt
    19561 ggtcgtgcga ggcaccgacg tggccctcat gcgcgacggc gaccggatcc acgccgtcgg
    19621 cgggacctgt ccgcaccgcg gcggccagat cgctgacggt cgggtggtcg acggcgaggt
    19681 catctgtccg ctgcacctgt gggacttcga cctgcgcacc ggcatctcgg cgttcaaccc
    19741 gaaggaccga ctgccccact tcgacgcccg ggtccgcgac ggtcgcgtcg agatcgacgc
    19801 cgacagcgtg ccggcaggtc ccggacgccc ggacgtgtac ctggggccgt gggtccgccg
    19861 cggggcgacc gaccggggca tggacctcgt ccaccacctc gccgagggtg gcagccccta
    19921 cgtcgagtcg atgggctcga cccggctcca gccggactcc gcgccgatgg gtccgcaccc
    19981 atcgctcgac gacctggtgt tcctgcccgc gcagctcgcc cggttgccgc ttctcgacga
    20041 cgagcccgtg gccacctcgg tccgcctcgg cacgcgggca gcgcggccgc tcgacctggc
    20101 cgtcccgctg gtcgtgtcgc acatgagctt cggtgcgctg tcggccgagg ccaaggtagc
    20161 cctggcgacc ggggcagcgg ccgccgggac agcgatcggc tccggtgaag gcggcatgct
    20221 ccccgaggag cgcgccgcgg cggggctgta cgtgctcgag atggcgtcgg gctacttcgg
    20281 ctggaccgag gatgccgtcg ccgcggccga cgcggtcgag atcaagatcg gccagggggc
    20341 caagcccggc ctcggcggcg tcctgcccgc ggccaaggtc accgagcgga tcgccgaggt
    20401 ccggggcatc gaggcgggcg tcgacagcca ttcgccgtcg cggttccccg acatcggctc
    20461 acccgccgcg ctcgccgagc gcctcgagcg gatccgttcg atcaaccccg gcatcccgat
    20521 cggcatcaag atcgcggccg ggcgcgtcga cgacgacgtc gccgtcgccg tcgacgtcgg
    20581 agccgactac ctggtcgtcg acggcttcgg cggcgggacc ggagccgccc cggtgcacgt
    20641 gcgcgaccac gtcggcatcc cgagctgggt ggcgctgcac cgcgcccggg cctggctcga
    20701 cgaccacggc cacgccgacg tccagctggt ggtgaccggg ggctaccgga cgcccgacga
    20761 gatggccaag gcgatggccc tcggggccga cgccgtcgct ctggccaccg catcgatgat
    20821 ggcgatcggc tgtcagcagt accgggcgtg tcaccgaggc acctgtccgg tcggcatcgc
    20881 cacgcaggac ccggtgctgc gcagccgact ggatccggcg gtctcggccg agcgcctcac
    20941 ccgcttcctc ctcgctgcga ccgagatgat gaccgactac gcccgcatca ccggccggcg
    21001 ctccctgggc gagctcgccc gagaggacct ggccgcgctg cgccctgacg cggcggccct
    21061 gctcgacatc gacgtccggg cctgagtggt ccgggccccc cggatcgggc cggcctcggg
    21121 gtgccagtcg ccgttcggag ggtgtgacgg gagccgatgt cgccgtcggc gcccgggccc
    21181 tagtgttcct ccatggctgc acccgccgac tctgcccgcc cctccctgtc ggtgacgatc
    21241 tggtccgtct ggtcgtggtt cgcgttcggg ctgagcgtga tcgtgttcat cccgatcatc
    21301 ctcaccgtgt acgtggtgac gttcccgttc gaccggaccc actactgggc ggggtacatg
    21361 taccggaaga tgccggtgct gcaccagaag ctcaccccga tgtggaactt ccacgtgcgc
    21421 ggcgagctgc cggccaaccc ccgcaacccc tacgtggtgg tcgccaacca cgagtccttc
    21481 gtcgacatcc tgctgatcag tcacctgccg ttcgagatga agtggctgtc caagcaggag
    21541 atgttcaaga tcccgatcgc cggctggctg atgtacgtcg ccggtgacat caagttggat
    21601 cgcggggacc gggattcggc caagaaagcc atggaccgtt gcgtctggta cctgaagcac
    21661 cgcgtgtcgg tgatggtctt ccccgaaggc acccgggccg tcgacgggga gctcggcaag
    21721 ttcaaggacg gtgcgttccg cctggcgatc gaggcccagg tgcccatcct cccgctggcg
    21781 gtgcacggca ccaaggaggc gctgcgcaag cacgactggc gcttcggtcg ggccaacgcc
    21841 gcggtgcagg tcatggagcc ggtcagcacc gaggggttga ccctcgagga cgtcgattcg
    21901 ctcaaggagc gggtgaggga cctgatctca gatcagctcg acgtgatgga gcaggacccc
    21961 accctgtcga actgaccgct cggcgccctg ccgatgttgt agctggcggt cgctgtggtg
    22021 gcgtgtgggc tacaagttcg g
//
LOCUS       DAOQOD010000032        21641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_825_length_21641_cov_11.045029, whole genome shotgun sequence.
ACCESSION   DAOQOD010000032 DAOQOD010000000
VERSION     DAOQOD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_825_length_21641_cov_11.045029"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..779
                     /locus_tag="PLZ72_05095"
     CDS             234..779
                     /locus_tag="PLZ72_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35707.1"
                     /translation="MDHSADEVIPTADPTLDDEPSRPARSRLTPPRVWAGLMVLCLVV
                     AMTCFLAAGVIRRTDSASGTVGDALATFGGVFLATSLFAFVGFVFGAARESSDEARTE
                     HGVRSALVASGIVFVMALVGGGMVVAQTVEERLELDPATTAPTSGPKGSGTSSTSVAP
                     GAPAPAPTVATCPPDALPGTC"
     gene            complement(802..3015)
                     /locus_tag="PLZ72_05100"
     CDS             complement(802..3015)
                     /locus_tag="PLZ72_05100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNA35708.1"
                     /translation="MATVTRVLPWRRHTQPPAMEVQEIVEQYRRFHPKLPTESITEAY
                     RVAAGSHKGQLRRSGEPYITHPLAVAGIVARYGMDDVTISAALLHDAVEDTVLTLEEV
                     EAGFGSEVREIVDGVTKLERVHFDSKEAQQAATMRKMLVAMAKDLRVLVIKLADRLHN
                     MRTLAALPAWKQERTAQETLDIYAPLAHRLGMQEVKQQLEDLCFAALHPKRYAEIDHM
                     VANRTPERDRFLVEILDEVTGRLKDVNIAAEVTGRPKHLWSIYEKMVVKGKAFDEIFD
                     LVGIRVVVDSSKDCYAALGTIHALWTPVPGRFKDYIAMPKFNLYQSLHTTVIGPGGRP
                     LEVQVRTPEMHHRAEWGVAAHFSYKEGHSEDVAWLTRIVDWQQEMTDPGEFMANLKVD
                     LDQDEVFVFTPQGDVIALPVGATPVDFAYAIHTEVGHRCIGARVGGRLLPLDSPLSSG
                     DTVEIFTSKVAGAGPSRDWLDFVATRSASNKIRQWFSRERREDALESGRDALIKALRR
                     EGLPVQRILGGSAMSDVSDQLRFQSVDAMYVAIGEHHLSPEAVAARVGRLVRTAEGER
                     EEVISTPVRPTNGRRRSRPAGVHVEGYDDVLVRMARCCTPVPGDAIMGFVTRGRGVSV
                     HRVDCSNATSLLDGHQERVIDVDWDTESVGDFVALVEVKALDRPRLLQDVTATLSENH
                     VNIISAQTHSGGDRVAKMRFEFELGDASHLDTLLNRIRSIDSVYDAHRVVPGSAD"
     gene            complement(3128..4363)
                     /gene="secF"
                     /locus_tag="PLZ72_05105"
     CDS             complement(3128..4363)
                     /gene="secF"
                     /locus_tag="PLZ72_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNA35709.1"
                     /translation="MGLASDLYRGRNDFDFPRLWVRVGIVSTVLIIISLASMVTRGLN
                     LSIDFEGGSVWEVPSETFTEAQARTALADFGDSAVERFQEATTTEGGRVIRVSGRVDN
                     VAEGAKAADALAEASGLEKGEVKVNTVGPSWGSDITSQARLSLIVFMVLVAAYIAWRL
                     ETRMAIAAMVAVIHDIIITVGVYSVFQIEVTPATVISFLTILGFSLYDTIVVYDRVQE
                     NATRFGRSGQYTYTSIMRRSLNQVFMRSLNTTMVTIIPVISILVVGQIVFGQETLGDF
                     SLALLIGLVSGTYSSLFVAPPVTAWLKEREPRWRQIRERLVAKGVDVSDTTWHVASAG
                     APAAATSGVPARPRRGATGTASRPTRSSTTAVEDRSTDPVSSTDPAEATVGDADAAPA
                     PRNPYGSVPPRPRKTKKKR"
     gene            complement(4365..6248)
                     /gene="secD"
                     /locus_tag="PLZ72_05110"
     CDS             complement(4365..6248)
                     /gene="secD"
                     /locus_tag="PLZ72_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNA35710.1"
                     /translation="MTKHPLRYVIATMVVAYGLVAVTIALGKTPTLGLDLQGGISVNL
                     QPVKDGKVDDSVSPEQLDQAIGIIRKRVDALGVAEPEVSRQGNTIIVQLPGATDQQQV
                     LDIVGKTAELRFRPVLADVGVIPKGKDRKKAEAEIAKLRKELKIPEGVTAAQVATEEQ
                     QKQAAANPQTTPEAPPTTEAPTTTAAPTTTAGPATTVDPTRSGGNRSRLTGQQGGTTT
                     TTAPATTTTAPATTTTTTPPPLNQWGVNAFDSKFGQLYQLEQALGTQTTPPEDDKADA
                     EVNLADKDGNVMKLGPTMLTGRAVETATAGLGQDGRWQVNPVFRKGKDGIDLFNKAAA
                     LCNSGAAQCPGSNGQPGRLAIVLDSEVLSAPTINNPTFDRDSISISGAFDEDEAKSLA
                     VALRFGSLPIELQAQQAETVSATLGADALQAGIIAGAIGLFLVCAYLVFYYRLLGLAT
                     VGSLTISASILWVVMCWINATVTLAGVVGIVVSIGISLDSSIVFFETIKEDVRNGAGL
                     RPSVDKAFGAAYSTIVKADMSSLIGAGVLYWLSVGPVRGFAFYLGVATILDLISAYVF
                     LRPAALVLARSHHGEHPRRFGIPTDDLAPAVRARAESEPVPATATGGAAPSGDGASSG
                     EEA"
     gene            complement(6348..6863)
                     /gene="yajC"
                     /locus_tag="PLZ72_05115"
     CDS             complement(6348..6863)
                     /gene="yajC"
                     /locus_tag="PLZ72_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNA35711.1"
                     /translation="MNALAPLLAAESQGSPLGILILIVPFGLLIWLMVVPQRKQKARQ
                     QELISSLSVGDEVMTTGGIVGQITHIEDGLVHLEIDHDVVIRVAKGSIGRSMAEPDPD
                     AAPAKGGLLGGMFGGGAAASRERDADAVDVRSSTAKGAASKGGASKGTGVGAARSASS
                     KGTAKAGSRKK"
     gene            complement(6925..8061)
                     /gene="tgt"
                     /locus_tag="PLZ72_05120"
     CDS             complement(6925..8061)
                     /gene="tgt"
                     /locus_tag="PLZ72_05120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNA35712.1"
                     /translation="MKLTIDVEHRDGRARTGTVHTARGAFRTPLFMPVATRGSIRALM
                     THELTGLAAADGTRAEVLLANTYHLMLRPGAEVVAELGGLAAFSGWDGLTLTDSGGYQ
                     VFSLGPKLHDGGAEFRSTYDGSTHLLTPEGAVATQELLGADIQMVLDVCAALPSSRVA
                     LRDALETTASWAARARAAHRRTGNQALFGIVQGGTETDLRAESAQRTVELDFDGYAVG
                     GLSVGETREEMLPALDAALEHLPVDQPRYLMGVGDPLSIVEAVARGVDMFDCVLPTRL
                     ARHGTLLTDAGRLNIKRAEFARSEDPIDPNCPCPTCATYARGYLRHLTAVGEPAAASL
                     CTVHNLTWMLGLVARIRAAIAAGRLDGLRAEVAGVWSGSGPGLG"
     gene            complement(8058..9107)
                     /gene="queA"
                     /locus_tag="PLZ72_05125"
     CDS             complement(8058..9107)
                     /gene="queA"
                     /locus_tag="PLZ72_05125"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNA35713.1"
                     /translation="MRTDELDYDLPEGAIAQVPIEPRDAARLLVDRGPGRDPEHRTVA
                     ELPELVRPGDLLVLNDTRVLPARVPIERASGGTGEVLLLEERDDGWWEVLCRPARKLR
                     PGDVVDATAGGLRFEIGPDVGDGRKLVRPHSDVPLLDALEHAGEMPLPPYIGERLTDA
                     ERYQTVFSARPASAAAPTAGLHLTDRLLAELTRRGVATAAVELVVGLDTFRPLSTERV
                     EDHAIHTERYSVPAATWEAVERTRADGGRVVAVGTTSVRALESRATRGEPSGRTDLFI
                     TPGYRFAVVDAMVTNFHLPRTSLLALVQAFVGPRWRDLYATALADGYRFLSFGDAMLL
                     ERAEPRERADVGVVG"
     gene            complement(9129..10277)
                     /locus_tag="PLZ72_05130"
     CDS             complement(9129..10277)
                     /locus_tag="PLZ72_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35714.1"
                     /translation="MQRDHFTDEHDMFREAFRTFLDREMVPHREAWTEAEIVDRDVFA
                     KAGASGFLAMAVPEQYGGAGVDDFRYSQVIVEELTRADLYPHGLGMTLHNDVCLPYFL
                     DYTTDEQKQRWLPGIASGELITAVAMTEPGIGSDLASMTTTAIRDGDTYVLNGSKTFI
                     TNGIHADLVLVACKTDPTQKHKGMSIVVVERGMEGFERGRNLDKVGMHAQDTAELFFT
                     DVRVPVANRLGDEGQGFVLLVKNLPQERLSIAAAAVAHCRAMLTWTIDYAKERTAFGQ
                     PIGTFQNSRFKLAEMETEVTIAETFVDRCVELLNDGRLTAEEASMAKWWTTELQKRVA
                     DTGMQLHGGYGYMNEYPIARAWTDARITSIYGGTTEIMKEIIGRSMGF"
     gene            complement(10415..11524)
                     /gene="ruvB"
                     /locus_tag="PLZ72_05135"
     CDS             complement(10415..11524)
                     /gene="ruvB"
                     /locus_tag="PLZ72_05135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNA35715.1"
                     /translation="MAREELLDPSPHEGDDDVDRSTASQVAPGEGADLVDPSSDPVAT
                     LRPRTLDEFVGQTELKRRLAVILEAARRRGQAADHFLFAGPPGLGKTSLAGIVATEMD
                     VTMQVTSGPALERPGDLAAILTKLGDGDVLFIDEIHRLSRSIEEVLYPAMEDFQLDIV
                     LGKGPAARSIRLDLPRFCLVGATTRTGLITGPLRDRFGLVARLDYYEPDDLRAIVVRA
                     AGILGADIDGAGAEEIAGRSRGTPRIANRLLRRVRDVAEVEGDGRIDAATARAGLEMF
                     GIDSMGLDKVDRSILTSLCARFGGGPVGLSTLAIAVAEPPETVEDVYEPYLIQQGLLI
                     RTPRGRVATAAAWQHLGMVPPPSALPGTVDPSLFD"
     gene            complement(11568..12167)
                     /gene="ruvA"
                     /locus_tag="PLZ72_05140"
     CDS             complement(11568..12167)
                     /gene="ruvA"
                     /locus_tag="PLZ72_05140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNA35716.1"
                     /translation="MIGSLRGTLLDRDGPELLVEVGGIGYRLQVTPATAAGAGDVGAE
                     TFLHVHQQVREDAVTLYGFTTIEDRRVFEVLIGTHGVGPALGLAILSVHSPLELRRIL
                     ASDDVAALCLVPGVGRKTAARLLVELKSRMQAGEVDASAAVAAITGAPRGGTRADVRD
                     ALAELGYGADEIGRVLVDLPADGDTSELLREALQRLAAA"
     gene            complement(12164..12643)
                     /gene="ruvC"
                     /locus_tag="PLZ72_05145"
     CDS             complement(12164..12643)
                     /gene="ruvC"
                     /locus_tag="PLZ72_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNA35717.1"
                     /translation="MFVLGVDPGLSRCGYCVLRVGDGAPRAAALGVMRTDPDMDVPRR
                     LAELRDDFRGLLDEFEPRSVAVERVLFQVNVRTAMGVGQASGVLMAEAAARGAEVVEY
                     SPNQVKDAVAGHGDADKEQVQKMVQILLDLPAPPHPPDAADAAAVALTHVAMSGVLR"
     gene            12950..14020
                     /locus_tag="PLZ72_05150"
     CDS             12950..14020
                     /locus_tag="PLZ72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35718.1"
                     /translation="MTPVPAFGSDVAVVPPTATAPVTAPTDVGASAPVVQLSGVGHRF
                     GANDALRDLSFEVPDGAITVLLGPNGAGKTTAIRAITGALAPMWGTVRTFGLDPDDDG
                     HLVRPRCGVVSAKPALYDRLSGRDNLAYAAELYGVGADAADRIERCATRFGIEDALDQ
                     LVGGYSTGMKTRLALARSVLHDPQLLLYDEPTSGLDPESSYAVLDMIRDMTSYGHTVV
                     MCTHLLAEAEGLADHIVMIESGTDLISGSPTDLTRRYWPNPIVTLDAEDPRLLDRAAA
                     FPGVSGYRRDPAGARLEVDDLRRVPDMVASLIADGVRVTRVEPHVPTLEDLYFTIRRE
                     SGVGERSIAAPGGAARPDGGLR"
     gene            14017..>14550
                     /locus_tag="PLZ72_05155"
     CDS             14017..>14550
                     /locus_tag="PLZ72_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNA35719.1"
                     /translation="MTTIEFEDRNPAGATGPRRTARHTRRTRAARDFDRHRMWVVART
                     DLRQLVQSKDYWIPMVALGSLFFFVIPTILLLTITRIGDINVVQQASQALKILPAQAQ
                     AQIQGSSPQGRTAFALAVYLFAPVAVVVPLTISTAVGAATLVGERERGTGEFLAHSPA
                     SVREIYLGKLFASFVPGY"
     assembly_gap    14551..14650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14854..15780
                     /locus_tag="PLZ72_05160"
     CDS             14854..15780
                     /locus_tag="PLZ72_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35720.1"
                     /translation="MEESPDRRLQRWAAAHHGLYRATDALEAGLTRRQIRYRVEQGRA
                     DRIGYDLFRIAGAPVTQDQRTLAAAWRSGGVVSHRTAAELHDLIGRSIHTPHASVARR
                     SSHLLDDVVLHRTEDLLDVETMELRGVPVTTPARTLVDMGLQVTDKELEKALHRAVHT
                     GRTTFEEVWRIYRRISAQGRNGAGPIGDLLRSIDIDSAAAESDLEVELIAALRTFGAP
                     TPVRQHDVVVDGHRFRLDLAYPDHRVFLEGDGFGVHGDREGFELDRWRQDLLVVHGWW
                     PLRFTWRQIRRSPEWCALTVLRKLDQVGSPDF"
     gene            complement(15817..16335)
                     /locus_tag="PLZ72_05165"
     CDS             complement(15817..16335)
                     /locus_tag="PLZ72_05165"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA35721.1"
                     /translation="MSDPGAADRDPVDPADSDLRIGDPAPDFTLPGVDGRNGQSVEVT
                     LSDLRGRPVVVAFYPADDSPVCTAQLSEYTRTIGEFDRLDASVLAISPQSPESHRAFA
                     AAQDGFAFPLLSDEDLAVGRSFGVVGLLDLYRRSTFVIDAAGIVRYAHRYLNAGTGYQ
                     STPDLLTILSQI"
     gene            complement(16332..17081)
                     /locus_tag="PLZ72_05170"
     CDS             complement(16332..17081)
                     /locus_tag="PLZ72_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNA35722.1"
                     /translation="MSGHSKWATIKHKKGAADAKRGKLFAKLIRQVEVAARNGGGDPD
                     MNPTLRTMFQKARDASVPLDTIERAIKRGTGELEGVTYEEVTYEGYAPHGVAIYIEGL
                     TDNRNRTGPEVRSIFSKQGGSIAEPGAVAWQFERKGIVIVPGSVDEDEVMMAAIDAGA
                     DDVSGDGEHWQVTCEAQLTAAVRTGIEEAGITVESSDLTMLATSTVELDSVEAAKAVL
                     RLVDALEDHDDVQHVYANMDIADDVLAAIDA"
     gene            complement(17098..17676)
                     /gene="pdxT"
                     /locus_tag="PLZ72_05175"
     CDS             complement(17098..17676)
                     /gene="pdxT"
                     /locus_tag="PLZ72_05175"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNA35723.1"
                     /translation="MTVGVLALQGAFGLHVDLLESLGVRAVEVRRADQLDSVDRLVLP
                     GGESTTMSMLAERFDLLEPLRDFCRSGRPVFGTCAGAILLGSEILDGRDDQHCLGAVD
                     ISVRRNGFGRQVDSFEIDVDVDALADAGLGDAPFHAVCIRAPVIERVGDGVEVMARID
                     GSPALVRDGAIMVAIFHPELAGDRRLHELFVG"
     gene            complement(17673..18587)
                     /gene="pdxS"
                     /locus_tag="PLZ72_05180"
     CDS             complement(17673..18587)
                     /gene="pdxS"
                     /locus_tag="PLZ72_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNA35724.1"
                     /translation="MSQPGTPSLSGADHQTGSTLVKRGLAEMLKGGVIMDVVNAEQAR
                     VAEDAGATAVMALERVPADIRVDGGVARMSDPVMIEGIQAAVTIPVMAKARIGHFVEA
                     QILQALGVDYVDESEVLTPADETHHIDKWAFTVPFVCGATNLGEALRRISEGACMIRS
                     KGEAGTGNIVEAVRHLRSITGDIRKLTQADSAELFEWAKQLRAPLPLVQEVAETGVLP
                     VPLFCAGGIATPADAALAMQLGAQAVFVGSGIFKSDDPAPRAKAIVEATTHFRDADIL
                     AKVSRGLGAPMTGIGMDQIEVTFADRGW"
     gene            complement(18725..19573)
                     /locus_tag="PLZ72_05185"
     CDS             complement(18725..19573)
                     /locus_tag="PLZ72_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35725.1"
                     /translation="MSATPPRSDGSRTAEPGSDPARTAGFDARTGSVVTAWSLFGIMP
                     GMVVAVVVAIVAAPLLGLLALVVVAGAWALYVRLQVRGALDRVLGQVGARPLPSEDEP
                     RWANVVDGLGATSGVNDPELWVIDGVEANALAAASEDRAVLVVTRGLVDSLTVVELEG
                     VAANLLGRIKDGSARYGTVVVGLLGLFLGTVDAAGKILADGLGDQRAVHTDLAAVAMT
                     RYPPGLAAALEHLERIGTERPGVPPATAHLWMAPVIEGDVGIDPAVAATVAQPLDYRA
                     AVLHEL"
     gene            complement(19585..20682)
                     /locus_tag="PLZ72_05190"
     CDS             complement(19585..20682)
                     /locus_tag="PLZ72_05190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA35726.1"
                     /translation="MRIGLVCPYSLTMPGGVQGQVLALARSMRRRGHETRVLAPCDGP
                     PPELFVTPLGNSLPTAANGSVAPVAPDPAAQFRTIRALWDEAFDVVHLHEPLAPGPTV
                     TSLLLKPAPLVGTFHAAGSQPAYTAMKPLVQWGARRLDLRVAVSEDARDLARDHLDGD
                     YELMFNGVEVQRFASAEPWPTDGPTVLFVGRHEPRKGLDVLLSALEELPAEVRIWVAG
                     TGPDTDALQARFTDERIEWLGRIDDHERDRRLAGADVFCAPSLGGESFGVILLEAMAA
                     RTPVVASDIPGYTKVATAPAPDGSVPPEPAALLAPVGDAAALADRLRRVLGDPAEAAR
                     QRDLGWERVQPFDVERLTDLYLQRYHQLVAG"
     gene            complement(20693..>21641)
                     /locus_tag="PLZ72_05195"
     CDS             complement(20693..>21641)
                     /locus_tag="PLZ72_05195"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HNA35727.1"
                     /translation="LSVGGLRLASRLVRVLPEPMAAFAARGLAQAAVPLSADQRLIAE
                     RNMRRVLGPDASDTQVRRAVRHVFESYARYWVDTLRLPDLDADQVSDGFTIEGLEHIA
                     DALDRGVGPILALPHVGGWEWAGRWLGSEHGWTVTAVAETLEPPELHEWFLELRRGLG
                     MQIIPLGPGAGSESAAALARGDIMCLLCDRDLSGTGIEVEFFGERTTLPGGPALMALR
                     SESPLLPTAVYFRGRRCHGVVGAPLDTERRGRLRDDVARVTQDLAFAMEDLIRVAPEQ
                     WHLLQPNWPSDVVALGAADGTGPAADGSGPAAEGSGVPQ"
BASE COUNT         3043 a         7577 c         7823 g         3098 t
ORIGIN      
        1 ccgtgcatgc ggccgcgcac gcgaccagat agcgaagacc cctcatcacc cctagctccc
       61 ctctcgggcg acagccatgc gcccgatgtc acaagtcaac ctcatctccg aacggcgatc
      121 aaggagcagg tggtgagccg atcggtcgca cccgtggtca tggggtcggt tccgttccgg
      181 tggcgtacca tcgatgaccc accagatccg tcgaaacccg ggagaggacc ccgatggacc
      241 acagtgccga cgaggtgatt cccaccgccg atccgacgct cgacgacgag ccgtcgcgcc
      301 cggcacggtc acggctcact ccaccgcggg tgtgggcggg gctgatggtc ctgtgcctgg
      361 tggtggcgat gacgtgcttc ctcgccgccg gcgtgatccg ccgcaccgac tccgcatcgg
      421 gaaccgtcgg cgacgccctc gcgaccttcg gcggggtctt cctcgccacg agcttgttcg
      481 cgttcgtcgg cttcgtcttc ggcgccgccc gcgagtcctc tgacgaggcc cggaccgagc
      541 acggtgtccg gtccgcgctg gtcgcgtccg ggatcgtgtt cgtgatggcg ctcgtcggcg
      601 gcgggatggt cgtggcccag accgtcgagg agcgactcga actggacccg gcgaccaccg
      661 ccccgacgag cgggccgaag gggtccggca cgtcgtcgac ctcggtcgcc ccgggagctc
      721 ccgccccggc acccaccgtc gccacgtgtc cgcccgacgc gctgcccggt acctgctgac
      781 cggccggagc gggaggggtc ctcagtcggc tgagccggga accacccggt gggcgtcgta
      841 cacgctgtcg atgctgcgga tgcggttgag cagggtgtcg aggtgggagg cgtcgccgag
      901 ctcgaactcg aagcgcatct tggccacccg atcgccgccc gagtgggtct gggcgctgat
      961 gatgttcacg tggttctcgc tgagcgtcgc ggtcacgtcc tggagcaggc ggggccggtc
     1021 gagcgccttg acctcgacca gcgccacgaa gtcgccgacc gactcggtgt cccagtcgac
     1081 gtcgatcact cgctcctgat ggccgtcgag caacgacgtg gcgttggagc agtcgacccg
     1141 gtgaaccgac acgccccgac cgcgggtgac gaagcccatg atggcgtccc cgggaacggg
     1201 tgtgcagcag cgggccatgc gcaccagcac gtcgtcgtag ccctcgacgt gcacacccgc
     1261 cggccgggac cggcgccgac cgttggtggg ccgcaccggg gtggagatga cctcttcgcg
     1321 ctcgccctcc gcggtgcgca ccagccgacc gacccgtgcc gcaactgctt cgggcgacag
     1381 gtggtgctcg ccgatggcga catacatggc gtcgacggac tggaagcgca actggtccga
     1441 cacgtcggac atggcgctgc cgcccaggat cctctggacc ggcaggccct cacggcgcag
     1501 cgccttgatc agagcgtcgc ggccggactc cagggcatcc tcgcgccgct cgcgggagaa
     1561 ccactggcgg atcttgttcg acgcggaccg ggtggcgacg aagtcgagcc agtcccggct
     1621 gggtccggcc ccggccacct tcgacgtgaa gatctcgacc gtgtcacccg acgacagcgg
     1681 actgtcgagc ggtagcagtc gaccgcccac ccgtgcgccg atgcagcggt ggcccacctc
     1741 ggtgtggatg gcgtaggcga agtcgaccgg ggtggcgccg accgggaggg cgatgacgtc
     1801 gccctgcggg gtgaacacga agacctcgtc ctggtccagg tcgaccttca ggttcgccat
     1861 gaactcgccg gggtcggtca tctcctgttg ccagtcgacg atccgcgtca gccaggcgac
     1921 gtcctcggag tggccctcct tgtacgagaa gtgggccgcc acgccccatt cggcccgatg
     1981 gtgcatctcg ggggtgcgga cctgcacctc gagcggacgt ccgcccgggc cgatcaccgt
     2041 cgtgtgcagc gactggtaca ggttgaactt gggcatggcg atgtagtcct tgaaccgccc
     2101 gggaaccggg gtccacagcg cgtggatggt tcccagcgcc gcgtagcagt ccttggacga
     2161 gtcgaccacg acccggatcc cgaccagatc gaagatctcg tcgaacgcct tgcccttgac
     2221 gaccatcttc tcgtagatgg accagaggtg cttcggccgt ccggtcacct cggcggcgat
     2281 gttgacatcc ttcagccgtc cggtgacctc gtcgagaatc tcgaccagga aacggtcacg
     2341 ttccggggtc cggttggcga ccatgtggtc gatctcggcg taccgcttgg ggtgcagcgc
     2401 ggcgaagcac aggtcctcga gctgctgctt gacctcctgc atccccaagc ggtgcgccag
     2461 cggggcgtag atgtcgaggg tctcctgggc ggtgcgctcc tgcttccagg ccggcagcgc
     2521 ggccagggtc cgcatgttgt ggaggcggtc ggccagcttg atgaccagga cccggaggtc
     2581 cttggccatc gccaccagca tcttgcgcat ggtggcggcc tgctgggcct ccttggagtc
     2641 gaagtggacc cgctccagct tggtcacgcc gtcgacgatc tcgcgcacct cggacccgaa
     2701 gccggcctcg acctcctcga gtgtcagcac cgtgtcctcg acggcgtcgt gcaggagcgc
     2761 ggcggagatg gtgacgtcgt ccatgccgta gcgggcgacg atgcccgcca ccgccaacgg
     2821 gtgggtgatg tagggctcgc cggagcgacg cagctggccc ttgtgggacc ccgccgccac
     2881 ccggtaggcc tcggtgatcg actcggtggg cagcttgggg tggaaccgtc ggtactgctc
     2941 gacgatctcc tgcacctcca tggccggagg ctgggtgtgg cgccgccacg gcaggacgcg
     3001 cgtcaccgtc gccatgtcgg accctcctgg cgcccaggcg ccgccgggga cggcgtcgat
     3061 cgctggtcgt gccgcacgtc caccacgcta ccgagcccgc ccggagcgcc gtacccgacc
     3121 gtgcggctca gcgcttcttc ttggtcttgc ggggacgggg cggcaccgag ccgtacgggt
     3181 tccgcggcgc gggtgcggca tcggcatccc cgacagttgc ttcggccggg tcggtcgacg
     3241 agacggggtc ggtggaccga tcctcgaccg ccgtcgtcga cgagcgggtc ggccgggagg
     3301 cggtgccggt ggccccgcgt cgcggacggg cggggacgcc ggacgtcgcc gcggccgggg
     3361 caccggcgct cgcgacgtgc caggtcgtgt cggagacgtc gactcccttg gcgaccaggc
     3421 gttcgcggat ctgacgccag cggggctcgc gctccttgag ccacgcggtg accggcgggg
     3481 ccacgaacag cgacgagtag gtccccgaca cgaggccgat cagcagcgcc agcgagaagt
     3541 cgccgagcgt ctcctggccg aacacgatct ggcccaccac caggatcgag atgacgggga
     3601 tgatcgtcac catcgtggtg ttgagcgacc gcatgaacac ctggttcagc gaccgtcgca
     3661 tgatcgaggt gtaggtgtac tggccggatc ggccgaaacg ggtcgcgttc tcctggacgc
     3721 ggtcgtacac gacgatcgtg tcgtagagcg agaaccccag gatcgtcagg aacgagatga
     3781 ccgtcgccgg ggtgacctcg atctggaaca ccgagtagac gccgacggtg atgatgatgt
     3841 cgtggatcac cgccaccatc gcggcgatcg ccatccgggt ctccaaccgc caggcgatgt
     3901 aggcggcgac cagcaccatg aacacgatga gcgacaggcg ggcctgcgag gtgatgtcgc
     3961 tcccccagga ggggccgacg gtgttgacct tgacctcgcc cttctcgagt ccggaggcct
     4021 cggcgagggc gtccgcggcc ttggccccct cggcgacgtt gtcgacccgc ccggacaccc
     4081 ggatgacccg gccgccctcg gtggtggtgg cctcctggaa ccgctcgacg gcgctgtcgc
     4141 cgaagtcggc cagagccgtg cgggcctggg cctcggtgaa ggtctccgag ggcacctccc
     4201 acaccgatcc gccctcgaag tcgatcgaca ggttgaggcc tcgggtgacc atcgacgcca
     4261 gcgagatgat gatgagcacg gtcgagacga tcccgacccg cacccacagg cgggggaagt
     4321 cgaagtcgtt gcgcccgcgg tagagatccg acgccaggcc catgtcaggc ctcctctccg
     4381 gacgatgcgc cgtctccgga cggtgctgct ccgccagtcg cggtggccgg caccggctcg
     4441 gattccgctc gggcacggac ggcgggcgcc aggtcgtcgg tggggatccc gaagcgcctc
     4501 gggtgctcac cgtggtggga gcgggccagc accagggcgg ccgggcgcag gaacacgtac
     4561 gccgagatca ggtcgaggat ggtggccacg cccaggtaga aggcgaagcc gcgcaccggg
     4621 ccgaccgaca gccagtacag gacgccggct ccgatcagcg agctcatgtc ggccttgacg
     4681 atcgtcgagt aggccgcgcc gaacgccttg tcgaccgacg gtctcaggcc cgctccgttg
     4741 cgtacgtctt ccttgatcgt ctcgaagaac acgatggagc tgtcgaggga gatgccgatc
     4801 gacaccacga tgcccacgac gccggccagg gtgacggtcg cgttgatcca gcacatcacc
     4861 acccacagga tcgacgccga gatggtgagg ctgccgaccg tggccagccc gaggaggcgg
     4921 tagtagaaga ccaggtaggc gcacaccagg aacagaccga tcgccccggc gatgatgccg
     4981 gcctgcagcg cgtcggcgcc cagcgtcgcg gacacggtct cggcctgctg ggcctgcagc
     5041 tcgatcggca gcgagccgaa ccgcagcgcg acggccaggc tcttggcctc gtcctcgtcg
     5101 aaggcgcctg agatcgagat cgagtcccgg tcgaaggtgg ggttgttgat cgtcggcgcc
     5161 gacagcacct cggagtccaa cacgatggcg agtcgcccgg gctggccgtt gctgccgggg
     5221 cactgcgccg cacccgagtt gcacagcgcg gctgccttgt tgaacaggtc gatgccgtcc
     5281 ttgcccttgc ggaacaccgg gttgacctgc cagcgaccgt cctggcccag cccggccgtt
     5341 gcggtctcga ccgcccgccc ggtcagcatg gtcggaccga gcttcatgac gttgccgtcc
     5401 ttgtcggcca ggttgacctc ggcgtcggcc ttgtcgtcct cgggcggggt ggtctgggtg
     5461 ccgagcgcct gctcgagctg gtacagctga ccgaacttgg agtcgaaggc gttgaccccc
     5521 cactggttga gcggcggcgg ggtggtggtc gtggtggtgg ccggtgcggt cgtcgtggtg
     5581 gccggtgcgg tcgtcgtggt ggtgccgccc tgctggccgg tcaggcggct gcggttcccg
     5641 ccggatcggg tggggtcgac ggtggtggcc ggacccgcgg tcgtggtcgg cgccgcggtc
     5701 gtggtcggcg cctcggtcgt cgggggcgcc tcgggggtgg tctgcgggtt ggcggccgcc
     5761 tgcttctgct gctcctcggt ggcgacctgg gccgcggtga ccccttcggg gatcttcagc
     5821 tccttgcgga gcttggcgat ctccgcctcg gccttcttgc ggtccttgcc cttggggatc
     5881 acgccgacgt cggcgaggac gggccggaag cgcagctcgg cggtcttgcc gacgatgtcg
     5941 aggacctgct gctggtcggt ggcaccgggg agctgcacga tgatcgtgtt gccctggcgg
     6001 gagacctcgg gttcggccac gccgagggcg tcgacgcgct tgcggatgat gccgatggcc
     6061 tgatcgagct gctcgggcga gaccgagtcg tcgaccttgc cgtccttgac cggctggagg
     6121 ttgaccgaga tgccgccctg gaggtcgagg ccgagggtcg gggtcttgcc cagagcgatg
     6181 gtgaccgcga ccaggccgta ggccacgacc atggtggcga tcacgtatct caacgggtgc
     6241 ttggtcattc tcgtcctcgt cgatggggcc gggtcagggt accggcccga cggcgggacg
     6301 cggatgatgc gggccgccct tcccgccttc ccccggggtg ggaccgccta cttcttgcgg
     6361 gagcccgcct tggccgtccc cttggaggag gcgctgcggg ccgcgccgac gccggtcccc
     6421 ttggacgcgc ctcccttgga agcggctccc ttggcggtcg agctgcggac gtcgacggcg
     6481 tcggcgtcgc gctcgcggga tgctgcggca cccccaccga acatgccgcc gagcaacccg
     6541 cccttggccg gggcggcgtc ggggtcgggt tcggccatcg agcgaccgat cgagcccttg
     6601 gccacccgga tcaccacgtc gtggtcgatc tcgaggtgga ccaggccgtc ctcgatgtgg
     6661 gtgatctgac cgacgatgcc gccggtggtc atgacctcgt caccgaccga cagcgagctg
     6721 atcagctcct gctgacgggc cttctgcttg cgctggggca cgaccatcag ccagatcagc
     6781 aggccgaagg ggacgatcag gatcaggatg ccgagggggc ttccctggct ctcggcggcg
     6841 aggagggggg cgagcgcgtt cacgacccga gatggtaggg cggaggggtg cgacccgggc
     6901 aatcaggcgc gggggtcgcg acgctcaccc gaggccggga ccggatccgg accagacccc
     6961 ggcgacctcg gcgcgcagcc cgtccaaccg gccggcggcg atggcggccc ggatgcgggc
     7021 gaccaggccg agcatccagg tcaggttgtg gaccgtgcac agcgacgcgg cggcgggctc
     7081 accgaccgcg gtgaggtggc gcaggtagcc cctcgcgtac gtcgcgcacg tcgggcacgg
     7141 gcagttcggg tcgatcgggt cctcggagcg ggcgaactcg gcgcgcttga tgttgagccg
     7201 accggcgtcg gtcaacagcg tgccgtggcg ggccagtcgg gtgggcagca cgcagtcgaa
     7261 catgtcgaca ccccgggcga ccgcctcgac gatcgacagc gggtccccca cccccatcag
     7321 gtagcggggc tggtcgacgg ggaggtgctc gagcgcggcg tcgagggcag gcagcatctc
     7381 ctcgcgggtc tcgcccaccg acaggccacc gaccgcgtag ccgtcgaagt cgagctcgac
     7441 ggtgcgctgg gcggactcgg cgcgcaggtc ggtctcggtg ccgccctgca cgatgccgaa
     7501 cagcgcctgg tttccggtcc gccggtgtgc ggcgcgggcc cgggccgccc acgacgcggt
     7561 cgtctcgagc gcgtcgcgca gcgcgacccg gctcgacggc agcgccgcgc acacgtcgag
     7621 gaccatctgg atgtcggcgc cgagcagctc ctgggtggcg accgcaccct cgggggtgag
     7681 caggtgggtg ctcccgtcgt aggtggagcg gaactcggcg ccgccgtcgt gcagcttcgg
     7741 tccgagcgag aacacctggt agccgcccga gtcggtcagc gtgagtccgt cccagcccga
     7801 gaacgcggcc aggccgccga gctcggccac cacctcggcg ccggggcgca gcatcaggtg
     7861 gtaggtgttg gccagcagca cctcggcgcg ggtcccgtcc gccgcggcca ggcccgtcag
     7921 ctcgtgggtc atcagcgccc ggatcgagcc gcgggtggcg accggcatga acagcggcgt
     7981 gcggaacgcg cctcgagcgg tgtgcaccgt gccggtgcgg gcccgcccgt cgcggtgctc
     8041 gacgtcgatc gtgagcttca cccgaccacc ccgacgtcgg cccgctcccg cggctcggct
     8101 cgttccagga gcatcgcgtc gccgaaggac aggaaccggt agccgtcggc cagggccgtg
     8161 gcgtacaggt cacgccagcg ggggccgacg aacgcctgca ccaacgccag caggctggtg
     8221 cggggcaggt ggaagttggt gaccatcgcg tcgacgacgg cgaagcggta cccgggggtg
     8281 atgaacagat cggtgcggcc cgatggctcg ccgcgggtgg ctcgcgactc gagcgcccga
     8341 accgaggtgg tgcccaccgc caccacccgg ccgccatcgg cccgggtgcg ctcgaccgcc
     8401 tcccacgtgg cagccggcac cgagtatcgc tcggtgtgga tcgcgtggtc ctcgacccgc
     8461 tcggtcgaca gcggacggaa cgtgtcgagg ccgacgacca gctccaccgc cgccgtcgcc
     8521 accccgcgcc gggtcagctc ggcgaggagc cggtcggtca ggtgcagacc cgcggtgggc
     8581 gccgccgcgg acgcgggtcg ggccgagaac accgtctggt agcgctcggc gtcggtcagc
     8641 ctctccccga tgtacggcgg cagcggcatc tccccggcgt gctccagcgc gtcgaggagc
     8701 gggacgtcgc tgtggggtcg tacgagcttg cggccgtcac cgacgtcggg accgatttcg
     8761 aagcggaggc cgccggcggt ggcgtcgacg acgtcgccgg ggcgcagctt gcgggccggg
     8821 cggcacagca cctcccacca gccgtcgtcg cgctcctcga gcaggagcac ctcgcccgtc
     8881 cccccgctgg cccgttcgat cggcacccgg gccggcagca cgcgggtgtc gttgaggacc
     8941 agcaggtcgc cggggcgcac cagctcgggc agctcggcca cggtccggtg ctcggggtcc
     9001 cgtcccgggc cgcggtcgac caggagccgt gccgcgtcac gcggttcgat cggcacctgg
     9061 gcgatggcgc cctcgggcag gtcgtagtcg agctcgtcgg tgcgcatcgg ggccggcggg
     9121 tcggccggtc agaaccccat ggagcgaccg atgatctcct tcatgatctc ggtggtcccg
     9181 ccgtagatcg aggtgatgcg ggcgtcggtc caggcccggg cgatcgggta ctcgttcatg
     9241 tagccgtagc cgccgtgcag ctgcatgccg gtgtcggcca cccgcttctg cagctcggtg
     9301 gtccaccact tggccatcga cgcctcctcg gcggtgaggc ggccgtcgtt gagcagctcg
     9361 acgcagcggt cgacgaacgt ctcggcgatc gtcacctccg tctccatctc ggccagcttg
     9421 aagcggctgt tctggaaggt gccgatcggt tggccgaacg cggtgcgctc cttggcgtag
     9481 tcgatcgtcc aggtgagcat cgcccggcag tgcgccaccg cggccgccgc gatcgacagc
     9541 cgctcctggg gcaggttctt gaccagcagc acgaagccct ggccctcgtc gcccaggcgg
     9601 ttggccaccg ggacgcggac gtcggtgaag aacagctcgg cggtgtcctg ggcgtgcatc
     9661 ccgaccttgt ccaggttgcg gccccgctcg aagccctcca tgccccgctc gacgacgacg
     9721 atcgacatgc ccttgtgctt ctgggtgggg tcggtcttgc acgcgaccag gaccaggtcc
     9781 gcgtggatgc cgttggtgat gaaggtcttg gagccgttga gcacgtaggt gtcgccgtcg
     9841 cggatggcgg tggtggtcat cgacgccaaa tccgacccga tgcccggctc ggtcatggcc
     9901 accgcggtga tcagctcgcc agaggcgatg cccggcagcc agcgctgctt ctgctcgtcg
     9961 gtcgtgtagt cgaggaagta gggcaggcag acgtcgttgt gcagcgtcat gcccaggccg
    10021 tgggggtaca ggtcggcccg ggtgagctcc tcgacgatca cctggctgta gcggaagtcg
    10081 tcgacgcccg ctccgccgta ctgctcgggc acggccatgg ccaggaaccc ggaggcgccg
    10141 gccttggcga acacgtcgcg gtcgacgatc tcggcctcgg tccaggcctc gcggtgcggg
    10201 accatctcgc ggtcgaggaa cgtccggaac gcctcgcgga acatgtcgtg ctcgtcggtg
    10261 aagtggtcgc gttgcactgg gtgacctcgg gagtgggggt tgcggtggtg gggtggttgg
    10321 gtgcgatggc ccggccctgc gccggcaggt ccggaccgtt cagatgtgcc tcccagactc
    10381 ccacgcggac ggtcctgcga ccgaaccggg ccggtcagtc gaacaggctc gggtccaccg
    10441 ttccgggcag cgccgacggt ggcggcacca tcccgaggtg ctgccacgcc gccgcggtgg
    10501 cgacccgtcc ccggggcgtg cggatgagca ggccctgttg gatcaggtac ggctcgtaga
    10561 cgtcttcgac cgtctcggga ggttcggcca ccgcgatggc cagggtcgac aggccgacgg
    10621 gtccgccgcc gaagcgggcg cacagcgagg tgaggatcga ccggtcgacc ttgtcgaggc
    10681 ccatggagtc gatgccgaac atctcgaggc cggcgcgggc cgtcgccgcg tcgatgcggc
    10741 cgtcgccctc cacctcggcc acgtcgcgca cccggcgcag cagtcggttg gcgatgcggg
    10801 gcgtgccccg tgaccgaccg gcgatctcct cggcgccggc cccatcgatg tcggcgccga
    10861 ggatgccggc cgcgcgcacg acgatggcgc gcaggtcgtc gggctcgtag tagtcgagcc
    10921 gggccaccag gccgaagcga tcccgcagcg gcccggtgat cagcccggtg cgggtggtgg
    10981 ccccgaccag gcagaagcgg ggcaggtcca ggcggatcga gcgcgccgcg gggcccttgc
    11041 ccaggacgat gtcgagctgg aagtcctcca tggccgggta gaggacctct tcgatcgacc
    11101 gggagagccg gtggatctcg tcgatgaaca ggacgtcgcc gtcgcccagc ttggtgagga
    11161 tggcggccag gtcgcccgga cgttcgagcg cggggcccga ggtgacctgc atggtcacgt
    11221 ccatctcggt ggcgacgatg ccggcgagcg acgtcttgcc caacccgggc ggccccgcga
    11281 acaggaagtg gtccgcggcc tgacctcgac gccgggccgc ctccaggatg acggcgaggc
    11341 ggcgcttgag ctcggtctgg ccgacgaact cgtccagcgt gcgggggcgg agggtcgcga
    11401 ccggatcgga ggaggggtcg acgaggtcgg ccccctcgcc gggcgcgacc tgcgacgcgg
    11461 tggagcggtc gacgtcgtcg tcgccctcgt gcggcgacgg gtcgaggagc tcttcgcgtg
    11521 ccatcagcgg agccacccgc gagccggacc ggcgcagggt cgcgctctca cgcggccgcc
    11581 agcctctgga gcgcctcgcg cagcagctcg gaggtgtcgc cgtcggcggg caggtcgacc
    11641 agcacccgac cgatctcatc ggcgccgtag ccgagctcgg ccaacgcgtc gcgaacatcc
    11701 gcgcgggtcc cgccgcgggg cgctccggtg atggccgcca ccgcggcgga ggcgtcgacc
    11761 tcgccggcct gcatccggga cttgagctcg acgagcagcc gagccgcggt cttgcggccg
    11821 actccgggga ccaggcagag cgcggcgacg tcgtcggagg cgaggatgcg gcgcagctcc
    11881 agcggtgagt gcaccgacag gatggccagt ccgagggcgg gtccgacgcc gtgggtgccg
    11941 atcagcacct cgaagacccg tcggtcctcg atcgtcgtga acccgtaaag ggtcaccgcg
    12001 tcctcacgga cctgctggtg gacgtgcagg aacgtctcgg cgccgacgtc gcccgcaccg
    12061 gcggcggtgg ccggggtgac ctggagccgg tagccgatgc ctccgacctc gacgagcagc
    12121 tccggtccgt cgcggtcgag cagggtcccg cgcagcgagc cgatcaccgg agcaccccgc
    12181 tcatggcgac gtgggtgagc gcgaccgcgg cggcatcggc tgcgtcgggc gggtgcggcg
    12241 gagcggggag gtcgaggagg atctgcacca tcttctgcac ctgttccttg tcggcgtcgc
    12301 cgtggccggc gacggcgtcc ttcacctggt tgggcgagta ctcgacgacc tcggcgcccc
    12361 gggcggcggc ttcggccatg aggacccccg acgcttgacc gacgcccatg gcggtgcgca
    12421 cgttgacctg gaacaggacg cgctccaccg ccaccgaccg cggctcgaac tcgtcgagca
    12481 ggccgcggaa gtcgtcgcgg agctcggcga gccgcctggg gacgtccatg tcggggtcgg
    12541 tgcgcatgac gccgagcgcg gcggcccggg gcgcgccgtc gccgactcgc agcacgcagt
    12601 agccgcaccg cgacagcccg ggatccacac cgagtacgaa cacaggttcg accgtagcaa
    12661 ctgcgaccgg gtgagcgggg ggaccctcgc ggccgcgtga ccgggaccgc cgggtggcgg
    12721 atcggcccgg tcgcgcccac gtctcccacc acggccacgg catcacgagc tacagaacgc
    12781 ggcggacgcg ccgatacgac cagtgatgga gtccctcgga tcccccgctg ccccgccgcg
    12841 tcccaccacg gagggctccg gctcctcccc caccgcgctc gtccccccgc cgtaccacgg
    12901 gccgctgccg accccaccgg tgtcagccgt accgtcggtg ccgacaggcg tgacgccggt
    12961 gccggcgttc ggatccgatg tcgccgtcgt ccctccgacg gccacggccc cggtcaccgc
    13021 cccgaccgac gtgggcgcat cggctcccgt ggtgcagctg tcgggagtcg ggcaccgctt
    13081 cggcgccaac gacgcgctgc gggacctgag cttcgaggtg cccgacggcg ccatcaccgt
    13141 gctgctgggc ccgaacggcg ccggcaagac gaccgccatc cgggccatca ccggagccct
    13201 cgccccgatg tggggcacgg tgcgcacctt cggactcgac ccagacgacg acggccacct
    13261 ggtgcggccc cgctgcgggg tggtgtcggc caagcccgct ctctacgacc ggctgagcgg
    13321 acgcgacaac ctggcctacg cggctgagct ctacggagtc ggcgccgacg cggcggaccg
    13381 gatcgaacgt tgcgccaccc gcttcggcat cgaggacgct ctcgaccagc tcgtcggcgg
    13441 ctactcgacg gggatgaaga cccgcctggc gctggcgcgc tcggtgctcc acgacccgca
    13501 gctgctgctg tacgacgagc cgacctcggg tctggacccg gagtcctcct acgcggtgct
    13561 cgacatgatc cgcgacatga cctcctacgg ccacacggtg gtcatgtgca cccacctgct
    13621 ggccgaggcc gaggggctgg cggaccacat cgtcatgatc gagtcgggca ccgacctgat
    13681 ctccggttcg ccgacggacc tgacccgtcg ctactggccg aacccgatcg tcaccctcga
    13741 cgccgaggac ccgcggctgc tcgaccgggc ggccgcgttc cccggcgtgt cggggtaccg
    13801 acgggacccc gccggggccc gactcgaggt cgacgacctg cgccgcgtac ccgacatggt
    13861 cgcctcgctg atcgccgacg gcgtgcgggt gacccgggtc gagccgcacg tcccgaccct
    13921 cgaggacctc tacttcacca tccgccgcga gtccggggtc ggagagcgca gcatcgccgc
    13981 acccggcggc gccgcccgac ccgacggagg tctccgatga cgacgatcga gttcgaggac
    14041 cgcaacccgg ctggcgccac cggcccccga cggaccgcgc gtcacacccg tcgcacccgg
    14101 gccgcccggg acttcgaccg ccaccgcatg tgggtggtgg cccgcaccga cctgcgccag
    14161 ctcgtgcagt ccaaggacta ctggatcccg atggtggcgc tcggctcgct gttcttcttc
    14221 gtgatcccga cgatcctgct gttgaccatc acccgcatcg gcgacatcaa cgtggttcag
    14281 caggccagcc aggcgctcaa gatcctcccg gcccaggccc aggcccagat ccagggcagc
    14341 tcgccgcagg gccggacggc cttcgccctg gcggtgtacc tgttcgcacc ggtggcggtg
    14401 gtggtgccgc tgacgatctc gaccgcggtc ggcgccgcca ccctcgtcgg cgagcgggag
    14461 cggggaaccg gcgagttcct ggcccactcc cccgcctcgg tgcgcgagat ctacctgggc
    14521 aagctgttcg ccagcttcgt gcccggctac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn tggatcctgg gcgcgatcgc ctgggtggtc gcggcgatca gcctgcacac
    14701 cggcgtccga tccgtcaccc gggcccgcct cctcggcgtc gcccagtgat ataggggtcc
    14761 tcactcacat cccttgtcac aatcccgacc ccagaatcaa cgggtcgcgg tccccggtga
    14821 caactcactc cctgatcggt actcctgtcc tccgtggagg agagccccga tcgacgactg
    14881 cagcggtggg ccgcggcgca tcacgggctg taccgggcga ccgacgcgct cgaggccggc
    14941 ctcacccgac gccagatccg ctaccgggtg gaacagggac gggccgaccg gatcggctac
    15001 gacctcttcc ggatcgccgg cgctccggtg acccaggacc agcgcaccct ggccgcggcg
    15061 tggcgcagcg gcggcgtggt ctctcaccgg accgcagctg agctccacga cctcatcggc
    15121 cggagcatcc acacgccgca cgcctcggtg gcgcggcgca gctcgcacct gctcgacgac
    15181 gtcgtgctgc accgcaccga ggacctgctc gacgtcgaga ccatggagct gaggggcgtc
    15241 ccggtcacca cccccgcccg gacgctcgtc gacatgggcc tgcaggtgac cgataaggag
    15301 ctcgagaagg ccctgcaccg agcggtccac acgggtcgca ccaccttcga ggaggtgtgg
    15361 cggatctaca ggcggatctc ggcgcagggc cgcaacgggg cgggaccgat cggcgacctc
    15421 ctgcgatcga tcgacatcga ctccgccgcg gccgagagcg atctggaggt cgagctgatc
    15481 gccgcgctgc gcacgttcgg ggcaccgaca ccggtccgcc agcacgacgt cgtcgtcgac
    15541 gggcacaggt tccgcctcga cctggcctat cccgatcacc gcgtcttcct ggagggcgac
    15601 gggttcgggg tccacggcga ccgtgagggc ttcgagctcg accgctggcg ccaggacctg
    15661 ctcgtcgtcc acgggtggtg gccgctgcgg ttcacgtggc gtcagatccg tcgatcgccc
    15721 gagtggtgcg ctctgacggt gctgcgcaag ctggatcagg tcgggtcgcc ggatttctga
    15781 ggccgggatc gtgacaaggg atgtgagtgc ggacccctat atctgggaga ggatggtgag
    15841 gaggtcgggg gtgctctggt agccggtgcc ggcgttgagg tagcggtgcg cgtagcggac
    15901 gatcccggcg gcgtcgatga cgaacgtcga gcgccggtac aggtcgagca gtccgacgac
    15961 gccgaaggat cggccgacgg ccaggtcctc gtcggacagc aacgggaacg cgaacccgtc
    16021 ctgggccgcg gcgaacgccc ggtgcgactc gggcgactgc ggcgagatgg ccagcaccga
    16081 cgcgtcgagg cggtcgaact cgccgatcgt cctcgtgtac tcgctcaact gcgccgtgca
    16141 gaccggcgag tcatccgccg ggtagaaggc gaccaccacc ggccttcccc gcagatccga
    16201 cagcgtcact tcgaccgact ggccgttgcg accgtcgacg ccgggcagtg tgaagtccgg
    16261 agccggatca ccgatccgga ggtccgagtc agccgggtcg accggatccc gatcggcagc
    16321 gcccggatcg ctcacgcgtc gatcgcggcg agcacgtcgt cggcgatgtc catgttggcg
    16381 tacacgtgct gcacgtcgtc gtggtcctcg agcgcgtcga cgagccgcag cacggccttg
    16441 gccgcctcga cggagtcgag ctcgaccgtc gaggtggcca acatggtcag atccgacgac
    16501 tcgacggtga tcccggcctc ttcgatgccc gtccggaccg ccgcggtgag ctgcgcctcg
    16561 caggtgacct gccagtgctc gccgtcgccg gacacgtcat ccgcgccggc gtcgatggcc
    16621 gccatcatca cctcgtcctc gtcgaccgag ccgggcacga tgacgatccc cttgcgctcg
    16681 aactgccacg ccaccgcacc gggctcggcg atcgagccgc cctgcttgga gaagatcgag
    16741 cgcacctcgg ggccggtgcg gttgcggttg tcggtcagac cctcgatgta gatcgccaca
    16801 ccgtgcggtg cgtagccctc gtaggtgacc tcctcgtagg tgacgccctc gagctcaccg
    16861 gtcccccgct tgatcgcccg ctcgatggtg tccagcggca ccgatgcgtc ccgcgccttc
    16921 tggaacatgg tccgcagcgt cgggttcatg tcggggtcgc caccgccgtt gcgcgccgcc
    16981 acctcgacct ggcggatcag cttggcgaag agcttgcccc gcttggcatc cgccgcgccc
    17041 ttcttgtgct tgatcgtcgc ccacttggaa tgcccggaca tagcgctcct gttcgcgtca
    17101 cccgacgaag agctcgtgca ggcggcggtc gccggcgagc tccggatgga agatggccac
    17161 catgatcgcg ccgtcgcgca ccagagcggg actcccgtcg atgcgcgcca tgacctcgac
    17221 gccgtcgccg acccgttcga tgaccggggc ccggatgcac acggcgtgga acggcgcgtc
    17281 gccgaggccg gcgtcggcca gcgcatcgac gtcgacgtcg atctcgaagc tgtcgacctg
    17341 acgcccgaac ccgttgcgcc gcactgagat gtccaccgcc cccaggcagt gctggtcatc
    17401 ccggccgtcg aggatctccg agcccagcag gatcgccccg gcgcacgtcc cgaacacggg
    17461 tcgacccgac cggcagaagt cgcgcagcgg ttcgagcagg tcgaagcgct cggccagcat
    17521 cgacatggtg gtggactcgc cgccgggcag gacgaggcgg tcgacggagt cgagctggtc
    17581 ggcccggcgc acctctacgg cgcggacccc gagcgactcg aggaggtcga cgtgcaggcc
    17641 gaacgccccc tgaagggcga ggaccccgac ggtcaccaac cgcggtccgc gaacgtgacc
    17701 tcgatctggt ccatgccgat gccggtcatg ggcgcaccca ggccccggct caccttggcg
    17761 aggatgtcgg cgtcgcggaa gtgggtggtg gcctcgacga tggccttggc ccgcggcgcg
    17821 gggtcgtcgg acttgaagat cccggagccg acgaacacgg cctgcgcacc cagctgcatg
    17881 gccagcgcgg cgtccgcggg agtggcgatg cctccggcgc agaacagcgg caccggcagc
    17941 acgccggtct cggcgacctc ctgcaccagc ggcagcgggg cccgcagctg cttggcccac
    18001 tcgaacagct cggcggagtc ggcctgggtc agcttgcgga tgtcgccggt gatcgaacgg
    18061 aggtgacgca ccgcctcgac gatgttgccg gtgccggcct cgcccttgga gcggatcatg
    18121 caggcaccct cggagatgcg ccgcagcgcc tcgcccaggt tggtcgcccc gcacacgaac
    18181 ggcacggtga acgcccactt gtcgatgtgg tgggtctcgt cggcgggggt cagcacctcg
    18241 gactcgtcga cgtagtccac gccgagcgcc tggaggatct gggcctcgac gaagtgcccg
    18301 atgcgggcct tggccatgac cgggatggtc accgcggcct ggatgccctc gatcatcacc
    18361 gggtcgctca tgcgggccac cccgccgtcg acgcggatgt cggcgggcac acgctcgagc
    18421 gccatcaccg cggtcgcccc cgcgtcctcg gccactcggg cctgctcggc gttgacgacg
    18481 tccatgatga cgccgccctt cagcatctcg gcgaggccgc gcttgacgag ggtcgaaccg
    18541 gtctggtggt cggcgccgga gagggagggg gtgcctggct gggacatggc cgcagtctac
    18601 cgacgagccc ggggtcgacc cgaagcggga aaccccaccc cgaacccgtt ctgtagtgcg
    18661 agatccacac gttcgcctgg atctcgcact acagaacggc ggtcagcggc gccgcgaacg
    18721 ggtctcagag ctcgtggagg accgcagcgc ggtagtcgag gggctgggcg acggtcgccg
    18781 ccaccgcggg atcgatcccg acatcacctt cgatcaccgg cgccatccac aggtgcgcgg
    18841 tcgccggtgg gacccccggt cgctcggtcc cgatccgctc gaggtgctcc agcgccgcgg
    18901 ccagacccgg cgggtagcgg gtcatggcca ccgcggccag atcggtgtgc accgcccgct
    18961 ggtcgcccag tccgtcggcc aggatcttgc cggcagcgtc gacggtgccg aggaacaggc
    19021 cgagcaggcc gaccacgacg gtgccgtacc gggccgagcc gtccttgatg cgtcccagca
    19081 ggttggcggc gacgccttcg agctccacga cggtcagcga gtcgaccaga ccgcgggtca
    19141 ccaccagcac ggcgcggtcc tcggacgccg cggccagcgc gttggcctcg acgccgtcga
    19201 tcacccagag ctcggggtcg ttgaccccgc tggtggcgcc gagcccgtcg acgacgttgg
    19261 cccaccgcgg ttcgtcctcg gacggcagcg gtcgggcgcc gacctggccg agcacccggt
    19321 cgagcgcgcc gcgcacctgg aggcgcacgt acagagccca cgccccggcg acgaccacca
    19381 gggcgagcag cccgaggagc ggagcggcca cgatcgccac cacgacggcg acgaccatgc
    19441 cgggcatgat cccgaacagc gaccaggcgg tgacgaccga gcccgtccgg gcgtcgaagc
    19501 ccgcggtccg ggccggatcg gagcccggtt cggcggtccg tgacccgtcg gagcggggag
    19561 gcgttgcaga cacggcggtc caggctaccc ggcgacgagc tggtggtacc gctgcaggta
    19621 aaggtcggtg aggcgctcga cgtcgaaggg ctgcacccgc tcccagccga ggtcccgctg
    19681 ccgggcggcc tccgccgggt caccgagcac ccgtcgcagc cgatcagcca acgccgcggc
    19741 atcgccgacc ggggcgagca gcgcagcggg ttcgggtggg accgacccgt cgggcgccgg
    19801 ggcggtggcg accttggtgt agccggggat gtcgctggcc accaccggcg tccgggccgc
    19861 catggcctcg agcaggatga cgccgaagga ctccccgccg agcgacgggg cgcagaacac
    19921 gtcggccccg gccagtcggc ggtcccgctc gtggtcgtcg atgcgaccga gccactcgat
    19981 gcgctcgtcg gtgaagcggg cctgcagggc gtcggtgtcg ggtcccgtgc ccgccaccca
    20041 gatccgcacc tcggcgggca gctcctccag cgcgctcagc aggacgtcga ggcccttgcg
    20101 gggctcgtgt cgtccgacga acagcaccgt cgggccgtcg gtgggccacg gctcggcaga
    20161 ggcgaaccgc tgaacctcca ccccgttgaa catgagctcg tagtcgccgt cgaggtggtc
    20221 ccgagcgagg tcgcgggcgt cctcggacac cgcgacgcgc aggtccagac ggcgggcacc
    20281 ccactgcacc agcggcttca tggcggtgta ggcgggttgg gagcccgcag cgtggaaggt
    20341 gccgacgagc ggggcgggct tgagcaggag cgacgtgacc gtcgggccgg gggcgagcgg
    20401 ctcgtggagg tgcacgacgt cgaacgcctc gtcccacagc gcacggatgg tgcggaactg
    20461 agcggcgggg tcgggggcga ccggggcgac cgagccgttg gccgcggtgg gcaggctgtt
    20521 gcccagcggg gtcacgaaca gctcgggcgg cggaccgtcg cacggggcca gcacacgggt
    20581 ctcgtgcccg cgtcgacgca tggagcgcgc cagggcgagg acctggccct gcacgccgcc
    20641 gggcatggtc aggctgtacg ggcagaccag gccgatgcgc atcggcggca ccctactgcg
    20701 ggacgccgga gccctcagcg gccggaccgg agccgtcggc ggccgggccg gtcccgtcgg
    20761 cggcgcccag cgccacgacg tcgctcggcc agttgggctg gagcaggtgc cactgctcgg
    20821 gggcgacccg gatcaggtcc tccatggcga aagcgaggtc ctgggtgacc cgggcgacgt
    20881 cgtcgcgcag ccgcccccga cgctcggtgt cgagcggggc tccgaccacg ccgtggcagc
    20941 gacgaccccg gaagtagacc gcggtcggca gcagcggcga ctccgagcgc agcgccatca
    21001 gggccggccc gccgggcagc gtggtgcgct cgccgaagaa ctcgacctcg atgcccgtgc
    21061 ccgacaggtc ccggtcgcac agcaggcaca tgatgtcgcc gcgggcgaga gccgcggccg
    21121 actcggagcc cgctcccggc ccgagcggga tgatctgcat gcccagtccc cggcgcagct
    21181 ccaggaacca ctcgtggagc tcgggcggct cgagcgtctc ggccaccgcg gtgaccgtcc
    21241 agccgtgctc gctgccgagc caccggccgg cccactccca gccgcccacg tgaggcagcg
    21301 ccaggatggg cccgacgccg cggtcgaggg cgtcggcgat gtgctcgagc ccttcgatcg
    21361 tgaagccgtc ggaaacctgg tcggcgtcga ggtcgggcag ccggagggtg tcgacccagt
    21421 agcgggcgta gctctcgaac acgtggcgca ccgcccggcg gacctgggtg tcggatgcgt
    21481 ccgggccgag cacccggcgc atgttgcgct cagcgatcag gcgctggtcg gccgacagcg
    21541 gcaccgcggc ctgagccagt ccccgggccg cgaacgccgc catgggttcc gggaggacgc
    21601 gcacgagccg cgacgccagc cggagtccac cgacggacag t
//
LOCUS       DAOQOD010000033        21336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_841_length_21336_cov_12.194775, whole genome shotgun sequence.
ACCESSION   DAOQOD010000033 DAOQOD010000000
VERSION     DAOQOD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 21336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21336
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_21336_cov_12.194775"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..832)
                     /locus_tag="PLZ72_05200"
     CDS             complement(23..832)
                     /locus_tag="PLZ72_05200"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNA35728.1"
                     /translation="MADQNDRGADDPIDLTDGSVDDRRLATIRGLLAKAEATEFPEEA
                     EAFFNKASELIARYAISEALIWASTSRGREHPDELRLVVHSPYVAQKSVLVGRVAAAH
                     GCRAIRFGSGSARDGEVVAVIGFPTDLRWIETLVTSLLVQLTAAMLARCPRGLTSAQS
                     AGWRRSFIIGFSEEVGNRLQADREVAARDADAFDDGGRRGDRNGHSVSLVLVERSEEV
                     RDEFRKRFPHTQTTRASTGGSRAGHHAGRAAGRDAAITRESIGGPRGLRRG"
     gene            complement(911..1867)
                     /locus_tag="PLZ72_05205"
     CDS             complement(911..1867)
                     /locus_tag="PLZ72_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNA35729.1"
                     /translation="MDGSTAGGCTDPWRLGPSDRDQPAGRVLPLVLGTYLEDFREAEP
                     AVRSDGDPEDLHRFRVSLRRARSLMAVGSDVLPSEELVLLQALASWFANVTSPVRDLD
                     VLLVDLDDLGRSVVPELHDALDELRVAVAAQRADAFDALVAELDGDRYPVLLRRWQAM
                     TAVVRVGGSDPGPDARRPMGEVSDQLIWSSYRRLRRRGRRAMSTDDLDAWHDLRKAIK
                     RFRYLVAAFAPMYPDSAFEQVPRRLADLQDTLGRLQDHHVQAAWIETIGVSAGGRAAL
                     AAGVVANTLHERSDRAHDHCREAWSEFDRPKLRRRLRVLLTA"
     gene            complement(2009..4357)
                     /locus_tag="PLZ72_05210"
     CDS             complement(2009..4357)
                     /locus_tag="PLZ72_05210"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNA35730.1"
                     /translation="MTQTFAAEGGYQIFTLGSSEWFWLIFSMAVAAIALIAGYFMMQG
                     VLSKQTGTAEMEEIASAVQEGAMAYIKRQFRTILLIVVPLAVVVFATSTEVLNVSGTE
                     GLDRVQSGIFRTLAFLAGAAFSGFIGFLGMWLATRANIRTAAAAKTGSMDAALQVAFR
                     AGGTIGLFTAGLGLFGATLIVMVFQNTASAILVGFGFGGSLLALFLRVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDKRNPATIADNVGDNVGDCAGMASDLFESFGVVLVANIILGV
                     TAFRAIGIGGGNAAKGLIFPLAMMAVGLLASIVSIFLVKARPGETDALKPINRGLNVA
                     SVIALVGAAVIAFAYVGDPRGSEVSNVGLRMFIAIVAGVVLGQAASRITQYYTSSQFK
                     PVKDIAESGRTGPATVVLSGISSGMESAVWAVVAIAVAILVGLGLGGGSSLFAFYMVA
                     LTGIGMLSTTGIVVAEDTFGPIADNAQGIAEMSGELEGETERILDGLDTVGNTTKAVT
                     KGFAIGSAVIAAVALFASFRETIASTAMTEVGNAVRELDVAAAFDAVSINIADPKTFA
                     GALIGGTVVFLFSSLAILAVGRTAGTVVQEVRRQFREKPGIMDYSEKPDYGPVIDICT
                     GAALRELTTPALLAVLMPVVVGFGLGAMALGGYLAATIVVGALMANFLSNSGGAWDNG
                     KKYIEEGNLGGKGSDVHKAVVIGDTVGDPFKDTAGPAINPLIKVMNLVSLLVLPAILS
                     LSDNDVARYFIAAAALVVVIGSVIYSKSQSTSFGDDVAETTA"
     gene            complement(4551..5219)
                     /locus_tag="PLZ72_05215"
     CDS             complement(4551..5219)
                     /locus_tag="PLZ72_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNA35731.1"
                     /translation="MLNLAPLFALLPDWLDPEKIISRGGYLLIFAIVFAESGLLIGFF
                     LPGDSLLFTAGMFAAGTFEDKLPNVQFNIWVLCLGVFIAAVAGDQVGYLFGRKAGPAL
                     FSRPDSRFFKQEHLEKAQSFFEHHGPRAIVLARFVPIVRTFCPIVAGAGQMEYGTFVR
                     FNVVGGFLWSVGVTLLGYFLGNVPLIADNIELALLMVVAVSLIPIAIEVIKSRRAKKA
                     HAAG"
     gene            complement(5245..8286)
                     /locus_tag="PLZ72_05220"
     CDS             complement(5245..8286)
                     /locus_tag="PLZ72_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNA35732.1"
                     /translation="MPDDMPRPPRRERKERGPRPNRARWALGILAGVLILLALSLRAI
                     ATFWTDFLWFDSLDLSAVWRRLLSARLTLGVAVTLVFFVILFVNLLVAERLAPRFRPV
                     VGPEDEVVVRYRQLVDGRQRVLSFGLALLVALVPGLSAANQWKAWLLFRFGGSFGQAD
                     EQFGTDIGFYVFKLPFLSLVVDWLFAFLLVTAVIVAVVHYLNGAIRLQPMGERITQNA
                     KAQLSVLLALAALVKAADYALQRFELTFASGSSFDGAGYTAVNARIPATEFLILISVF
                     VAVLFIVNIWRRGWIMPGIVVALWVLVALIVGSVYPAFVQRFQVSPAELSKERPFITR
                     NIDATREALGLGDVTEVAFDYQKTVTPGAIEAQRTNLADARLLDPAVIQPTVQELEFE
                     REFYRFDDIDVDRYVVEAADGQTPAPDRVPAIVSARELNAAGIKDATWEKLHLIFTHG
                     YGLALAPANSTNSRGEPDFLVWGIPAQTKGLPELKRPEIYHGEGMGGYAIVGTDQKEL
                     STDQVSTSYTGRSGVPLDSMARRAAFALRFGQIEPMISSNLTERSKVIYRRDVTERVN
                     AVAPYLTLDTDPYPVMVDGRIKYVIDAYTTADTYPYGQSIDASSIDARLQGSFNYMRN
                     SVKAVVDAYDGTVTLYLTDTLYGGKKDPIIRAWARAFPDLYQTDIPESLSRHFRYPEL
                     MFKVQTEAWGRYHQSDPSAFFNNSDRWSIAQQPPNSASTAVSQDPATGQTTTNLPRIE
                     PYYQMMQLTPDAPAEFLLTRPFVLSSNDESGRNLTSVMIASNDPGSYGQLRQIVMGNG
                     DSDSNGPKVDGPLQAHQKIVTYEPVSQYQTIVGRNGSSVRYGNMLILPFGNSLLYARP
                     IYAKAEQSGRYALTRVAVTNGDLVGFGETIDSAVADLLDGDDSGAVEQPADTLTPTEG
                     PAPSTTEPSDSAPSGSEGRSATELLADADARFDEADKKLKAGDLGGYQDAVADARDLM
                     RRAEQALSRSSASSTTAPSTTPSTTTPPAGAPTAAAPGGSG"
     gene            complement(8304..10694)
                     /locus_tag="PLZ72_05225"
     CDS             complement(8304..10694)
                     /locus_tag="PLZ72_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNA35733.1"
                     /translation="MTDLPADPVPDPPELDPGTLGSAEFGRTRKGFEPVEVRALLGRA
                     ADALRAWKERDDRLTARLADLERRLEQSQELDEQRITTVLGEETARIVAAAREAATEI
                     RTKAETQAAQLVADSEERAARLIADSEEEATATAARLTDEATALRDEAAAVRADAQEH
                     ADRVRAEASAEHDALLAKAKTVLDERTAEAEAVASGIREAAQIELDAARADGVRLRDE
                     ARVAADAEVERSRAEGRAMVDEARQLRKQMLQDLAERRRTARRQMEAARAGRDRIVES
                     LGAAADRVVQVIEDLAAGDDQIQRAADAAAAAVVDDIDEVVAELETGLGPGALPSGPD
                     AVDDVDAGDDGLDAQDAETADDADAGADPQDDAVASPELEVVDSDGPPTDADADVEPS
                     PGATVHDLFERIRAERRADSEAVHPSVDPSGGSADDDLDLYDDEEDDEGDESGDDDDG
                     TRGAEGSDDELAPVVVLEPADLVDGDGGLGDGGDDGDAGGADVVAIETTAVDAVIETT
                     VRESTVVAVVADSAADGVTVVETEVVETTTVETEVVAVDAVVDLDAAAELDVALEEAA
                     AESSALDRRDDLLGGVEKALSRSLKRLVSDEQNEVLDRLRRIRRGRVELEVLLPVGDD
                     LGIFAAALHDDFAAAAAAGARFWAAESDDDVDSAVARIDADVVEGGLEVRVADLLELR
                     RAHLARALESAESDGVELAEMADHVRAAYREWRTRSVPDLAGDLATAGFALGEQAAAG
                     DGTSWCWMVDNGGLPCSDAEDNALAGPTPCGSAFPTGDIVPPAHPGCRCILVPAPR"
     gene            complement(10806..12059)
                     /locus_tag="PLZ72_05230"
     CDS             complement(10806..12059)
                     /locus_tag="PLZ72_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNA35734.1"
                     /translation="MSAEPAGVEPVGDPSDPQHAGAESLDGAEPLDQTVRSTRSRRRR
                     WLWGGVGLAVLAVLAGVLFIPTPYYLFQPGSVRPAETRIDVSGAPSYTTDGDVMFTTV
                     YVDQASLATLLRGVIDDAVEIRSEEDVYGPEGRDASRRANQQRMDLSKLIATKVALQY
                     LGYPAEFTGRGARVLGVAEGGGSVGKLRVGDVITSVDGHDVRLPSDIQAGLRGKVPGD
                     TVEVTARRGSGKDVTTVSESITLGSARTEDDAASTTTRPPGSTTSSTVGSDGAAASPA
                     EATPGVRPVLGISVEPDAPSVDSPVRVDIDSGDVTGPSAGLAWALAVVDRMTPESLTD
                     GKEVAVTGEILPDGTVGPIGGIVQKVAAVKRAGVRTFLYPEATPDDEERQMREVAGGG
                     VRLVPVKTLDDAVVALHPGGVRRAN"
     assembly_gap    12147..12246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12391..12900)
                     /locus_tag="PLZ72_05235"
     CDS             complement(12391..12900)
                     /locus_tag="PLZ72_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35735.1"
                     /translation="MSQHMVIFQTADGSPAYSQFESVSEAVGFVESLRNDQGIENARM
                     FELKEIKYELKPIFKVELKELNPAPSAPASAQPQPQPAPAPAAAAAPAARPQPAAAPG
                     AGDTTSFGSFGQPGQPGQPGAPGPQPGAQPGSAPSTPDAAPAAPAPTPPADQPGSDAP
                     PARRGLFGR"
     gene            13075..13839
                     /locus_tag="PLZ72_05240"
     CDS             13075..13839
                     /locus_tag="PLZ72_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35736.1"
                     /translation="MADPIPDPPSDHPTGPATSVANTDREAVVLAGHRGDLVTVVDAL
                     AASEPSVRAAALGALGRIAARAPIDPDVVPDALARALADPDPTVRRRAAAEVARWAAT
                     CSEPTGAEPAGSEPTGAAPTPVVDGLLGRLTDDEVDTVTEVAAFACGELALDDRTRPR
                     VVTALDRLARTHADSLCREAAVAALGSIGDPAGLSAVLHGCSDRATVRRRAVLALAAF
                     DDPAATEALRRLVADRDLQVRQSAEELLAIESGETT"
     gene            complement(13903..15255)
                     /locus_tag="PLZ72_05245"
     CDS             complement(13903..15255)
                     /locus_tag="PLZ72_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA35737.1"
                     /translation="MRHRTRRVGLATTAALLAVVTLAATACGGRDDDSGSGSTTTAKE
                     GDSSSAAFIDPSVDCTDYQGTEGINGDTIKIGTVRPASGTYAIYDQVTTGLNAWVTYV
                     NQSGGLKAKDGKSYKLELIKEDDAYDPAKTPALVKKLVEQDKVFALVGDIGTEPNLSV
                     RDYLNEKCVPNIALATGSTQWGNNKQYPWYIAGLPSYATEAHAWIEYLKESNPKAKIA
                     LLYQDDDFGQAYEKAIKKAIKGTDITIVAEQGFNPLGGTTPEAAVTKLSQSDADTFIV
                     GLGGTPCPTSLKLKPDTWKPATIVSVTCAGKTALSLAGGKDQGVIAAQATYDPSDPAD
                     AQVPAVQQFRKDATAAGLTEQQIDGGITGPGWGFGSLFGLGIENAETLDRAGVMNALW
                     SLDTDSFGLVRDGVTVITDGTEDPWAIEGFRIVQREGDGWKELSPITNWEGKSNSFAG
                     "
     gene            complement(15278..16381)
                     /locus_tag="PLZ72_05250"
     CDS             complement(15278..16381)
                     /locus_tag="PLZ72_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNA35738.1"
                     /translation="MSDATALGDGPTAKVAPSPARRVPVGRVVVSVVVAAVLVALPFM
                     FPAFRVEQFVGWMALAIAACGLNLLTGYNGQISVGHGALYGTGAYATALLITDLGWPV
                     WPSVAAAGVICFVVGVAIGLPALRIKGLYLALVTLAVATLFPLIIEQFSSITGGGSGL
                     SITTPQETPRGVIERPIRLESPGGSLAPDQWKYFVFLVLTVVVFVVMRNIVRSRTGRS
                     LVAVRDNETAAEVSGVHVARVKILTFGVSSAMAGIGGAVFALNNGRINPSSFTIVVSI
                     YFLIAVVVGGAASVIGPAIGAVAYGLFIDVLSPELPERFKPATPVILGVLLILLMLFA
                     PGGIVGMWRTSAAKRAAKRARAATAAAASGDTV"
     gene            complement(16378..17262)
                     /locus_tag="PLZ72_05255"
     CDS             complement(16378..17262)
                     /locus_tag="PLZ72_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNA35739.1"
                     /translation="MTDFLQVLFVGIGDGAIYAMLGLGLVVIYRSTGLLNFAQGEMAM
                     FSTFIVWTVWDLGVPLPLAVIAGMVGGFGVGALAHQIAVRPVGDPHEKPLAVVMVTIG
                     LFVGLNSAAQLVWGTLDRDLPPLFGSGGIEVAGVAIAWQKIGAVIVLAVLALGFYLLF
                     QRTGLGLAMRAVASNSESASLVGVPVPKLLMLGWGLAAAVGTVAGVFTAPGRGLGSNM
                     MQLTLIFGFAAITLGGFDSLLGAVVGGFVVGIASSVVPQYVPGFEKMPLAPAFILILA
                     VLLFRPEGLFGRKQVQRV"
     gene            complement(17267..17974)
                     /locus_tag="PLZ72_05260"
     CDS             complement(17267..17974)
                     /locus_tag="PLZ72_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35740.1"
                     /translation="MSLLEVRGLRAGYGIVEVLFGLDLDVDAGELVVVLGANGSGKTT
                     TLRAVSGMLESRGEIVFDGRSLRGLRPDQVVQAGIAHVPQGRGTINDLTVVENLRIGA
                     WTRRDSEVAADIDLWCDAFPRLGERRDQLAGSMSGGEQQMLAIARAFMSRPRLLLLDE
                     PSLGLAPLVTAEVFDRLRDITDRTGTAVLLVEQNANLALDFAGRAYVLEAGEIVASGP
                     AAQLQHDETVQKAYLGI"
     gene            complement(18042..18806)
                     /locus_tag="PLZ72_05265"
     CDS             complement(18042..18806)
                     /locus_tag="PLZ72_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35741.1"
                     /translation="MAVLEVVDVGVRFGGVVALDSLSFTIDAGQICALIGPNGAGKTT
                     LFNVVSRIYEPTSGSVRFDGHDLLGLPAHRIAELGVGRTFQNLALVPGLSVLDNVKVG
                     GHARSRGGFWAAALRLPPMPAQERGLEARAMDVLERLSLAPLAQRPCAGLPYGTLKRI
                     EFARALVGQPKLLMLDEPASGLTHGEVDELGALIRQIRDEFSLTVLLVEHHMSMVMAI
                     SDKVVVMDLGRKIAEGLPAQVREDPKVIAAYLGAPA"
     gene            complement(19009..20013)
                     /locus_tag="PLZ72_05270"
     CDS             complement(19009..20013)
                     /locus_tag="PLZ72_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNA35742.1"
                     /translation="MARDLAIDLGTANTLVYARGEGIVLNEPSVIALNSRTNEVLAMG
                     RDAWKMIGRTPSYIVAVRPLRQGAITDFEVTQEMIRLLLKRVGVNRFNRPRVVICVPS
                     AITAVEKRAVTEAARKAGATEAQLIEQPMAAAIGAGLHIHEPMANMVVDIGGGTSETA
                     LISLGGIVALQAVRVGSFDIDASIITYIRREYGIAIGEQTAEEIKILIGSAYPTPDEV
                     RAEVRGRELMSGLPKTVILSAAEVRTAIAEPVNTMVDSVLSCVAEAPPELAQDLIERG
                     IFLVGGGSLLKGLDLRLAEETEVPVRMVRQPLESVVLGAGRVIESYEELRDMFMEARR
                     "
     gene            complement(20018..20212)
                     /locus_tag="PLZ72_05275"
     CDS             complement(20018..20212)
                     /locus_tag="PLZ72_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35743.1"
                     /translation="MSNLVFLGVALVISVVGVTILVLRSRPPSSPHSSIDQFHDKMRA
                     LAPDEPEIEGRVVRRRRTRR"
     gene            complement(20209..20748)
                     /locus_tag="PLZ72_05280"
     CDS             complement(20209..20748)
                     /locus_tag="PLZ72_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNA35744.1"
                     /translation="MPTSGTPSVSRPPERGDDWTALVAGGLPVGDAYAWAVRPDCGAV
                     VVFTGTARDHSAGRPEVTELAYEAYEDHAVARMDRVVDELRRRWPAAGRVAILHRVGD
                     VPIGQEAVVVAVSAPHRAEAFDAARFAIDAVKESVPVWKRERWAGGQDWGSDARVVVG
                     ADQVGADQVGPDPLGTTRP"
     gene            20833..>21336
                     /locus_tag="PLZ72_05285"
     CDS             20833..>21336
                     /locus_tag="PLZ72_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35745.1"
                     /translation="MQEPAERGPDGSDAAADGATARRWVHPSELGLQTRSRSDRRRGT
                     WLASTLVLGGLGLLAFGLIIGVGADRDPGTVASSPSHDVSATLASLTVLDGQSRRTVT
                     GVVLDAEGHVAVRSGSVSGAQEVWVSCGGAAPQAARVVAHDDGLDLAVVALGEPAGRP
                     VTAAGEAS"
BASE COUNT         3144 a         7491 c         7587 g         3014 t
ORIGIN      
        1 gggacccggt ccggcgggcc gttcagccac gacgcagtcc gcggggccca ccgatcgact
       61 cacgggtgat cgccgcgtcg cgtccggccg cccgccccgc gtggtgcccg gctcgggatc
      121 caccggtcga ggcccgggtg gtctgggtgt gcgggaaccg cttgcggaac tcgtcgcgca
      181 cctcctcgga tcgctcgacg agcaccagcg acaccgagtg gccgttgcgg tcgccccgac
      241 ggccgccgtc gtcgaaggcg tcggcgtcgc gggccgccac ctcgcggtcg gcctggagcc
      301 gattgccgac ctcttcggag aagccgatga tgaagctgcg acgccacccg gcggactgcg
      361 ccgaggtgag gccgcggggg catcgggcca gcatggcggc cgtgagctgc accagcaggc
      421 tggtcaccag cgtctcgatc cagcgcaggt cggtggggaa cccgatcacg gcgaccactt
      481 cgccgtcgcg ggccgacccc gagccgaacc ggatggcgcg gcaaccgtgc gcggcggcga
      541 cccgacccac cagcaccgac ttctgggcga cgtagggcga gtggacgacc aggcgcagct
      601 cgtcggggtg ctcccggccc cgtgacgtgc tggcccagat gagcgcctcg ctgatggcgt
      661 agcgggcgat cagctccgag gccttgttga agaacgcttc ggcctcctcg gggaactcgg
      721 tggcctcggc cttggcgagc agcccccgga tggtggccag acgtcggtcg tcgaccgatc
      781 cgtcggtgag gtcgatgggg tcgtcggccc cgcggtcgtt ctggtctgcc atgcgctcca
      841 gcctgacacg gcgctccgac atcggtcacc ggggagcgcg acccggtgtt cggcccggcc
      901 gggctgcgga tcaggccgtc agcagcacgc ggagacggcg cctcagcttc gggcggtcga
      961 actccgacca cgcctcccga cagtggtcgt gagcccgatc ggagcgctcg tgcagcgtgt
     1021 tggcgacgac gccggcggcc aacgccgccc ggcctcccgc gctgacgccg atcgtctcga
     1081 tccacgccgc ctggacgtgg tggtcctgca gccgacccag ggtgtcctgc aggtcggcga
     1141 gtcgacgtgg cacctgctcg aaggcgctgt cggggtacat gggggcgaac gcggcgacca
     1201 ggtagcggaa ccgcttgatc gccttgcgca ggtcgtgcca ggcgtcgagg tcgtcggtgg
     1261 acatggcccg ccggccccgg cggcgcaacc ggcggtacga cgaccagatg agctggtcgg
     1321 acacctcgcc catcggtcgg cgggcgtccg gtccgggatc gctgcccccc acccggacga
     1381 cggcggtcat cgcctgccag cgcctcagca ggaccgggta gcgatcgccg tcgagctcgg
     1441 cgaccagcgc gtcgaaggca tcggcgcgct gtgcggcgac ggcgacgcgg agctcgtcga
     1501 gggcgtcgtg cagctccggc acgaccgacc ggccgaggtc gtccaggtcg acgagcagga
     1561 cgtcgaggtc acggaccggc gaggtcacgt tggcgaacca cgacgccaac gcctgcagca
     1621 ggaccagttc ctcggacggc aggacgtcgg accccaccgc catcagcgac cgggcccgcc
     1681 gcaggctcac ccggaaccgg tgcagatcct cgggatcacc gtcggagcgg accgccggtt
     1741 cggcctcccg gaagtcctcg aggtacgtac cgaggaccag cggaaggacg cgaccggcgg
     1801 gctggtcccg gtccgacggc ccgagccgcc acggatcggt gcaccctccc gccgtcgaac
     1861 cgtccatcgg ttcagtctcg caggacgacg gcgcgatccg acgccaccat cggacgattt
     1921 cggtccctca tcggcgattc cgatgacagt tgagtaccgc ggttgtcgtg acttgtcgtc
     1981 agaaacctgg gtttggggat gggggggctc aggccgtggt ctcggccacg tcatcgccga
     2041 acgacgtcga ctgcgacttg gagtagatca ccgagccgat gacgaccacc agcgccgcgg
     2101 cggcgatgaa gtaccgggcc acgtcgttgt cgctcagcga caggatcgcc ggcagcacca
     2161 gcagcgacac caggttcatc accttgatca gcgggttgat cgccggaccg gcggtgtcct
     2221 tgaacgggtc gccgaccgtg tcgccgatga cgacggcctt gtgcacatcg gagcccttgc
     2281 cgcccaggtt gccctcttcg atgtacttct tgccgttgtc ccaggcaccg cccgagttcg
     2341 acaggaagtt cgccatcagg gcgccgacga cgattgtggc ggccaggtag ccgccgaggg
     2401 ccatggcgcc gaggccgaag ccgacgacga ccggcatcag caccgcgagc agcgccgggg
     2461 tggtcagctc ccgcagggcg gcgccggtgc agatgtcgat gaccggcccg tagtcgggct
     2521 tctcggagta gtccatgatc ccgggcttct cccggaactg ccgccgcacc tcctgcacca
     2581 cggtgccggc ggtccggccc acggccagga tggccagcga cgagaacagg aacacgaccg
     2641 tgccgccgat gagcgctccg gcgaaggtct tgggatcggc gatgttgatc gacacggcgt
     2701 cgaacgccgc ggcgacgtcg agctcgcgca cggcgttgcc gacctcggtc atggccgtcg
     2761 acgcgatcgt ctcgcggaac gacgcgaaca gggccaccgc ggcgatgacc gcggatccga
     2821 tggcgaagcc cttggtcacg gccttggtgg tgttgccgac ggtgtcgagg ccgtcgagga
     2881 tccgttcggt ctcaccctcg agctcgccgg acatctcggc gatgccctgg gcgttgtcgg
     2941 cgatcgggcc gaaggtgtcc tcggcgacga cgatgccggt ggtcgacagc atgccgatgc
     3001 cggtcagggc gaccatgtag aaggcgaaca gcgagctgcc cccgcccagg ccgaggccga
     3061 ccaggatggc gacggcgatg gcgacaaccg cccacaccgc ggactccatg cccgaggaga
     3121 tgcccgacag gacgacggtc gcgggaccgg tccgtccgga ctcggcgatg tccttgaccg
     3181 gcttgaactg gctggacgtg tagtactggg tgatccggct ggcggcctgg ccgagcacga
     3241 cgccggcgac gatggcgatg aacatccgca ggccgacgtt gctgacttcg gagccacggg
     3301 ggtcacccac ataggcgaag gcgatgaccg ccgctccgac cagggcgatg accgacgcga
     3361 cgttgaggcc ccggttgatc ggcttgaggg cgtcggtctc acccggacgg gccttgacga
     3421 ggaagatcga cacgatcgac gccagcaggc cgacggccat catggccagc gggaagatga
     3481 ggcccttggc ggcgttgccg ccgccgatgc cgatggcccg gaaggcggtg acgcccagga
     3541 tgatgttggc gaccagcacg acgccgaacg actcgaagag gtccgaggcc atgccggcgc
     3601 agtcaccgac gttgtcgccc acgttgtcgg cgatggtggc cgggttgcgc ttgtcgtcct
     3661 cggggatgcc ggcctcgacc ttgccgacca ggtccgcgcc gacgtcggcg gccttggtga
     3721 agatgccgcc gccgacccgc aggaacagcg ccagcagcga cccgccgaag ccgaagccga
     3781 ccaggatggc cgacgcggtg ttctggaaga ccatgacgat gagggtggcg ccgaacagcc
     3841 ccaggccggc ggtgaacaga ccgatggtgc caccggcgcg gaacgcgacc tgcagcgcgg
     3901 cgtccatgga accggtcttg gccgccgctg cggtgcggat gttggcgcgg gtggcgagcc
     3961 acatgccgag gaagccgatg aagcccgaga aggcggctcc ggccaggaac gcgagcgtgc
     4021 ggaagatgcc ggactggacc cggtcgagcc cctcggtccc cgacacgttc agcacctcgg
     4081 tcgaggtggc gaacaccacg acggcgaggg ggacgacgat cagcaggatg gtgcggaact
     4141 ggcgcttgat gtaggccatc gcgccttcct ggaccgccga ggcgatctcc tccatctcgg
     4201 cggtgccggt ctgcttgctc agcacgccct gcatcatgaa gtagccggcg atcagggcga
     4261 tggcggcgac cgccatggag aagatcagcc agaaccactc ggagctaccg agagtgaaga
     4321 tctggtagcc gccctcggcg gcgaaggtct gcgtcatggg tttccctcgt gatgtcgtcg
     4381 cggaccgggt gtcggtcggc gcgcgcccgc tgcgggatgc ggtgtgggcg cggaccgggg
     4441 acggttccgg tcccagcggt accggggcag ttcagtggac ggggcgcccg tccgtcaaaa
     4501 tcccggcggc ggaccgatcg gatcgacggc ccggaaccgt caccggtcga tcagccggcg
     4561 gcgtgcgcct tcttggcgcg ccgcgacttg atcacctcga tggcgatcgg gatcagcgac
     4621 accgccacca ccatcagcag ggcgagctcg atgttgtcgg cgatcaacgg cacgttgccc
     4681 aggaagtagc cgagcagggt gacgccgacg ctccacagga agccgccgac cacgttgaag
     4741 cgcacgaagg tgccgtactc catctgcccg gccccggcca cgatcggaca gaaggtccgc
     4801 acgatcggca cgaaccgggc cagcacgatg gcccgggggc cgtggtgctc gaagaagctc
     4861 tgcgccttct ccaggtgctc ctgcttgaag aagcgcgagt cgggacggct gaacagggcg
     4921 gggccggcct tgcgtccgaa caggtagccg acctggtcgc cggcgaccgc ggcgatgaac
     4981 acgcccaggc acagcaccca gatgttgaac tggacgttgg ggagcttgtc ctcgaaggtc
     5041 cccgcggcga acatcccggc ggtgaacagc agggagtcgc ccggcaggaa gaacccgatc
     5101 agaagccccg actccgcgaa gacgatggcg aagatgagca ggtagccgcc ccgggagatg
     5161 atcttctcgg ggtcgagcca gtccggcagc agcgcgaaca gaggggccag gttcagcacg
     5221 ggcgctcagg gtagcggggc gcgctcatcc cgacccaccg ggggccgcgg ccgtcggagc
     5281 gccggcaggc ggtgttgtcg tcgacggggt cgtcgacggt gctgtcgtgg acgaggccga
     5341 tgagcgcgac agcgcctgct ccgcccggcg catcaggtcc cgggcatcgg ccaccgcatc
     5401 ctggtagccg cccaggtccc cggccttcag cttcttgtcg gcctcgtcga agcgggcgtc
     5461 cgcgtcggcg agcagttcgg tggcgctgcg gccctcggaa ccggacggtg cggagtcgga
     5521 gggctcggtc gtggacgggg ccggcccctc ggtgggggtc agggtgtcgg cgggctgctc
     5581 gaccgctccg ctgtcgtcgc cgtcgagcag gtcggccacc gcggagtcga tcgtctcgcc
     5641 gaagccgacc aagtccccgt tggtcaccgc cacccgggtc agcgcgtagc ggccgctctg
     5701 ctcggccttg gcgtagatgg ggcgggcgta gagcaacgag ttcccgaacg gcaggatcag
     5761 catgttgccg tagcggacgc tcgacccgtt ccgaccgacg atcgtctggt actgcgacac
     5821 cggctcgtag gtgacgatct tctggtgcgc ctgcagcgga ccgtcgacct tgggtccgtt
     5881 cgagtcggag tcgccgttgc ccatcacgat ctggcggagc tgtccgtagg aaccggggtc
     5941 gttggaggcg atcatcaccg aggtgaggtt gcgaccgctc tcgtcgttgg aggacaggac
     6001 gaacgggcgg gtcagcagga actcggccgg ggcatcgggc gtcagctgca tcatctggta
     6061 gtacggctcg atgcggggca ggttggtggt agtctgaccg gtcgccgggt cctgggagac
     6121 agcggtggac gccgagttcg gcggctgctg ggcgatgctc cagcggtcgc tgttgttgaa
     6181 gaacgccgag gggtccgact ggtggtatcg accccaggcc tcggtctgga ccttgaacat
     6241 cagctccggg tagcggaagt gccggctgag cgactcgggg atgtcggtct ggtacaggtc
     6301 ggggaacgcc ctggcccagg cgcggatgat cgggtccttc ttgccgccgt agagcgtgtc
     6361 ggtcaggtac agcgtgacgg tgccgtcgta ggcgtcgacg accgccttga cgctgttgcg
     6421 catgtagttg aacgagccct ggagtcgggc gtcgatgctc gaggcgtcga tcgactgtcc
     6481 gtacgggtag gtgtcggcgg tggtgtaggc gtcgatgacg tacttgatgc ggccgtcgac
     6541 catgaccggg tagggatcgg tgtccagggt caggtagggg gcgacggcgt tgacccgctc
     6601 ggtgacgtcg cggcggtaga tgaccttgga gcgctcggtc aggttgctcg agatcatcgg
     6661 ttcgatctgt ccgaaccgca gggcgaacgc ggcgcggcgg gccatcgagt cgagcgggac
     6721 gcccgagcgc ccggtgtagg acgtggagac ctggtcggtc gacagctcct tctggtcggt
     6781 gccgacgatc gcgtagcccc ccatcccctc accgtggtag atctcgggcc gcttcagctc
     6841 cgggaggcct ttggtctggg ccgggatccc ccacaccagg aagtcgggtt caccccggct
     6901 gttggtcgag ttggccgggg ccagcgccag gccgtacccg tgggtgaaga tcaggtgcag
     6961 cttctcccac gtggcgtcct tgatgccggc cgcgttcagc tcgcgggccg acacgatggc
     7021 cgggacgcga tccggtgccg gggtctgtcc gtcggcggcc tcgaccacgt agcggtccac
     7081 gtcgatgtcg tcgaaccggt agaactcgcg ctcgaactcc agctcctgca cggtgggctg
     7141 gatgaccgcc gggtcgagca gtcgggcgtc ggccaggttg gtccgctggg cctcgatcgc
     7201 gccgggggtg acggtcttct ggtagtcgaa ggcgacctcg gtgacgtctc cgagcccgag
     7261 cgcctcccgc gtcgcgtcga tgttgcgggt gatgaacggc cgctccttgg agagctcggc
     7321 cggcgacacc tggaatcgct gcacgaacgc cgggtagacc gacccgacga tcagggcgac
     7381 cagcacccac agggccacga cgatgccggg catgatccag ccccgacgcc agatgttcac
     7441 gatgaacagc acggcgacga acaccgagat caggatcagg aactcggtcg ccgggatccg
     7501 ggcgttgacc gccgtgtagc ccgcaccgtc gaacgagctc cccgacgcga aggtcagctc
     7561 gaagcgctgg agggcgtagt cggcggcctt gaccagggcg gcgagggcga gcaggaccga
     7621 cagctgcgcc ttcgcgttct gggtgatgcg ttcccccatc ggctgcaggc ggatcgctcc
     7681 gttcaggtag tggacgaccg cgacgatcac ggcggtgacc agcaggaacg cgaagagcca
     7741 gtcgaccacc aacgacagga acgggagctt gaagacgtag aagccgatgt cggtgccgaa
     7801 ctgctcgtcg gcctgaccga aggacccgcc gaagcggaac agcagccacg ccttccactg
     7861 gttcgccgcg ctcagccccg ggacgagcgc gacgagcagg gccagaccga agctcaggac
     7921 acgctgtcgg ccgtcgacca gctggcggta gcggacgacg acctcgtcct cgggaccgac
     7981 caccggccgg aaccggggag ccaggcgttc cgccaccagc aggttcacga agaggatgac
     8041 gaagaacacc agcgtcaccg cgacgcccag cgtgaggcgg gccgacagca gccgtcgcca
     8101 gaccgcgctg aggtccaacg agtcgaacca caggaagtcg gtccagaagg tggcgatcgc
     8161 ccgaagtgac agcgcgagca ggatcaggac gccggccagg atcccgagag cccagcgggc
     8221 ccggttgggg cgcggccccc gttccttgcg ctcgcgccgc gggggccggg gcatgtcgtc
     8281 gggcatccgc acggcggatg aggttaccga ggagccggaa ccaggatgca tcggcatccc
     8341 gggtgcgccg gcgggacgat gtcgccggtg gggaaggcgg acccgcacgg cgtcgggccg
     8401 gccagggcgt tgtcctcggc gtcgctgcag ggcaggccgc cgttgtcgac catccagcac
     8461 cacgacgtcc cgtcgccggc cgcggcctgc tcgcccaggg cgaagcctgc ggtcgccagg
     8521 tcccccgcca gatcgggcac cgagcgggtc cgccactcgc ggtacgcggc acggacgtgg
     8581 tcggccatct cggccagctc gacgccgtcg gactcggcgc tctcgagagc gcgggccagg
     8641 tgcgcccgcc gcagctcgag caggtcggcg acgcgtacct cgaggccccc ctcgaccacg
     8701 tccgcgtcga tccgagccac cgcggagtcg acgtcgtcgt cggactcggc cgcccagaac
     8761 cgggctccgg cggccgcggc ggcggcgaag tcgtcgtgca gggcggcggc gaagatgccg
     8821 aggtcgtcgc cgaccggcag caggacctcg agctcgaccc gtccgcggcg gatccggcgg
     8881 agtcggtcga gaacctcgtt ctgctcgtcg gagaccaggc gcttgagcga tcgggacagt
     8941 gccttctcca ccccgccgag caggtcgtcg cgtcggtcga gggccgagga ctccgccgcc
     9001 gcctcctcga gcgcgacgtc gagctcggcg gcggcgtcga gatcgaccac cgcgtcgaca
     9061 gccaccacct cggtctcgac ggtcgtcgtc tcgaccacct ctgtctcgac gacggtgacc
     9121 ccgtcggccg cggaatcggc gaccacggcg acgacggtcg actcgcgcac cgtggtctcg
     9181 atgacggcgt cgaccgcggt ggtctcgatc gccaccacgt cggcgccacc ggcgtcaccg
     9241 tcgtcgccac catcgccgag cccgccgtcg ccatcgacga ggtcggcggg ctcgagcacc
     9301 acaaccgggg cgagctcgtc gtcggacccc tccgctccgc gagtgccgtc gtcgtcatcc
     9361 ccgctctcgt cgccctcgtc gtcctcctcg tcgtcgtaga ggtcgaggtc gtcgtcagcc
     9421 gacccgcccg acgggtccac cgacgggtgg acggcctcgg agtccgctcg acgttcggcg
     9481 cggatccgct cgaagaggtc gtgcaccgtg gcgcccggac tcggctcgac gtcggcatcg
     9541 gcatcggtgg gcggcccgtc gctgtcgacc acctctagct cgggcgacgc cacggcatcg
     9601 tcctgcgggt cggcaccggc atccgcgtcg tcggcggttt cggcatcctg cgcgtcgagg
     9661 ccatcgtcgc cggcatcgac gtcgtcgacc gcgtccggtc ccgacggcag cgcgcccgga
     9721 ccgagccccg tctcgagctc ggcgacgacc tcgtcgatgt cgtcgaccac cgccgccgcg
     9781 gccgcatccg cggcccgctg gatctggtcg tcgccggccg cgaggtcctc gatcacctgg
     9841 acgacccgat ccgcggcggc gccgagggac tcgacgatcc ggtcacgacc ggctcgtgcg
     9901 gcctccatct ggcgtcgggc cgtccgccgg cgttccgcca ggtcctgcag catctgcttg
     9961 cggagctggc gggcctcgtc gaccatcgcc cggccctcgg cccgggaccg ctccacctcg
    10021 gcgtcggccg cgacgcgtgc ttcatcacgt aggcgaacac cgtcggcgcg cgccgcgtcg
    10081 agctcgatct gcgccgcttc gcggatgccc gacgcgaccg cctcggcctc ggcggtcctc
    10141 tcgtcgagga cggtcttggc cttggccagc agggcgtcgt gctccgccga tgcctcggca
    10201 cggacgcggt cggcgtgctc ctgggcgtcg gcccggaccg cggccgcctc gtcgcggagg
    10261 gcggtggctt cgtcggtcag ccttgccgcg gtcgcggtgg cctcctcctc gctgtcggcg
    10321 atcaggcggg cggcgcgctc ctcgctgtcg gcgaccagct gagcggcctg cgtctcggcc
    10381 ttggtgcgga tctcggtggc cgcctcgcgc gccgcggcca cgatgcgagc ggtctcctcg
    10441 cccaggacgg tggtgatccg ctgctcgtcc agctcctggg actgctcgag gcgccgctcc
    10501 aggtcggcca gacgggccgt caggcgatcg tcgcgctcct tccaggcccg cagagcatcc
    10561 gcggcccgcc ccagcagcgc ccgcacctcg acgggttcga agcccttgcg ggtccgcccg
    10621 aactccgccg accccagggt cccgggatcc agttccggcg gatcgggcac aggatcggca
    10681 ggaaggtcgg tcatggccga atgctacggg cgcaccgccc ggcggtcgcg gacggccccg
    10741 gagcgatcgg acggaggttc cggacgcggc acgccgccgt cgtgagccgt ccgagcgggg
    10801 gcggttcagt tggctcggcg cacaccgccg ggatgaagcg ccaccacggc gtcgtcgagc
    10861 gtcttgaccg ggaccagccg gaccccacct ccggcgacct cccgcatctg gcgttcctcg
    10921 tcgtcggggg tggcctcggg gtacaggaag gtccggacgc ccgcccgctt caccgccgcc
    10981 accttctgga cgatgccgcc gatcgggccg accgttccgt cggggaggat ctcgccggtg
    11041 acggcgacct ccttgccgtc ggtcaacgac tcgggggtca tccggtcgac gacggccagc
    11101 gcccaggcca accccgccga cggaccggtc acgtctccgg agtcgatgtc cacccgcacc
    11161 ggggagtcga ccgagggagc gtcgggttcg accgagatgc cgagcaccgg ccggactccg
    11221 ggggtcgcct cggccggcga ggcggcggcg ccgtcggaac cgacggtgct cgatgtggtg
    11281 gatccgggcg gtctggtggt ggtcgacgcg gcgtcgtcct cggtgcgcgc cgagcccagg
    11341 gtgatcgact ccgacaccgt cgtgacgtcc ttgcccgaac cccggcgggc ggtgacctcg
    11401 acggtgtcgc cgggcacctt cccgcggagg ccggcctgga tgtcggaggg gagccgcacg
    11461 tcgtgtccgt cgaccgacgt gatgacgtcg ccgacccgga gcttgccgac cgaaccgccg
    11521 ccctcggcca caccgagcac ccgggcaccg cgaccggtga actccgccgg gtaccccagg
    11581 tactggaggg cgaccttggt ggcgatcagc ttggacaggt ccatccgctg ctggttggcc
    11641 cgacgcgagg cgtcgcgccc ttcggggccg tagacgtcct cctcggatcg gatctcgacc
    11701 gcgtcgtcga tgacgccccg cagcaacgtg gcgagcgacg cctggtccac gtacaccgtc
    11761 gtgaacatga cgtcgccgtc tgtcgtgtag gagggggcgc ccgacacgtc gatccgcgtc
    11821 tcggccggac ggaccgatcc gggctggaac aggtagtacg gcgtcgggat gaacaggacg
    11881 ccggcgagca ccgccagcac cgccagaccg actccgcccc acagccagcg gcggcgacgg
    11941 gaccgggtcg agcgcaccgt ctgatccagc ggctcggccc cgtccagcga ctcagccccg
    12001 gcgtgctgcg gatcggacgg gtcgccgacg ggttcgacac ccgcgggttc ggcgctcacg
    12061 tccgggtctg ctccggggcg ctccgacgga aggtcggcag tcggtacgct gggcggggtc
    12121 atgggtcggt caccgtcatc tcgtgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnntcgt ggtcgtgggc ttcttcaccg tcatcggctg agcccaccga ccgtcacccg
    12301 cggccgacgc cccgcggaca cccggacgcg accgcacgcc ggtcacccca cgaggaggtg
    12361 aaccggcgtg cggtccggga aacgatcgtc tcagcggccg aagaggccgc gccgcgccgg
    12421 cggagcgtcg gagccgggct ggtccgcggg tggcgtcgga gccggggcgg cgggagcggc
    12481 gtcgggggtc gacggcgccg agccgggctg agccccgggc tgcgggccgg gagccccggg
    12541 ctgaccgggc tgaccgggct ggccgaacga gccgaacgag gtggtgtcac ccgcaccggg
    12601 cgccgcagcg ggctgcggac gagcggccgg agccgcagcc gccgccggag cgggtgcagg
    12661 ctggggctgg ggctgggcag acgccggggc cgacggagcg gggttgagct ccttcagctc
    12721 gaccttgaag atcggcttga gctcgtactt gatctccttg agctcgaaca tccgggcgtt
    12781 ctcgatgccc tggtcgttgc gcagggactc cacgaacccg acggcctcgc tcaccgactc
    12841 gaactggctg tacgcggggc tgccgtccgc ggtctggaag atcaccatgt gttgcgacat
    12901 cgtctgggta cctctctcgt ccctcgtcgt ccgggggctt caatcctagg ggtggacacg
    12961 gccgagcgga ctcatccgac cgggtggttg actgaacggt agccgtccgg acaccccggc
    13021 gacgcccgtc acaccccgcg gtccgtccga accgaccgcg accgacaccg ccccgtggcc
    13081 gatccgatcc ccgacccacc ctccgaccac ccgaccggtc ctgcgacgag cgtcgcgaac
    13141 accgaccgcg aggccgtggt cctcgccggt caccgcggcg acctcgtcac cgtcgtcgac
    13201 gccctggccg cgtccgaacc atcggttcgg gccgcggcgc tcggtgccct cgggcgcatc
    13261 gccgcccggg cgccgatcga tcccgacgtc gtgcccgacg ccctggcccg tgcactcgcg
    13321 gatcccgacc ccaccgtccg gcgacgtgcc gctgccgagg tcgcccgatg ggcagcgacg
    13381 tgttccgaac cgaccggtgc cgaaccggcc gggtccgagc cgaccggtgc ggcgccgaca
    13441 ccggtggtcg acggcctgct cggccggttg accgacgacg aggtcgacac cgtcaccgag
    13501 gtcgccgcgt tcgcctgcgg tgagctggcc ctcgacgacc gcacccggcc gcgcgtcgtg
    13561 acggcgctcg accgactggc ccgcacccac gccgactccc tgtgccggga ggcggcggtg
    13621 gccgctctcg gcagcatcgg cgacccggcc gggctgtccg cggtgctcca cggctgctcg
    13681 gaccgggcaa ccgtacggcg gcgagcggtg ctggccctgg ccgccttcga cgacccggcg
    13741 gccaccgagg cgctccgacg gctcgtcgca gaccgcgacc tgcaggtccg ccagtccgcc
    13801 gaggagctcc tggccatcga gtcgggcgag acgacgtgac accggacgcg tgacggccgc
    13861 cccgaccaga gggtcggggc ggccgtcgtc ggaacgcagg gatcagccgg cgaagctgtt
    13921 ggacttgccc tcccagttgg tgatcggcga gagctccttc cagccgtcgc cctcacgctg
    13981 gacgatgcgg aagccctcga tcgcccacgg gtcctcggtc ccgtcggtga tcaccgtgac
    14041 gccgtcgcgc accaggccga agctgtcggt gtcgagcgac cacagggcgt tcatcacgcc
    14101 ggcccgatcg agcgtctcgg cgttctcgat cccgaggccg aacaacgagc cgaagcccca
    14161 tccgggaccg gtgatgccgc cgtcgatctg ctgctcggtg agcccggcgg ccgtcgcgtc
    14221 cttgcggaac tgctggacgg cgggcacctg ggcatcggcc gggtccgacg ggtcataggt
    14281 cgcctgggca gcgatcacgc cctggtcctt gccgccggcg agcgacaggg ccgtcttgcc
    14341 cgcgcaggtc accgacacga tcgtcgccgg cttccaggtg tcgggcttca gcttgagcga
    14401 cgtcggacac ggcgtgccgc cgaggccgac gatgaaggtg tcggcgtcgg actgcgacag
    14461 cttggtcacc gccgcctcgg gagtcgtccc gcccagcggg ttgaagccct gctcggcgac
    14521 gatcgtgatg tcggtgccct tgatcgcctt cttgatcgcc ttctcgtagg cctgaccgaa
    14581 gtcgtcgtcc tggtagagca gggcgatctt ggccttcggg ttggattcct tcaggtactc
    14641 gatccaggcg tgggcctcgg tcgcgtagga cggcagaccg gcgatgtacc aggggtactg
    14701 cttgttgttg ccccactggg tggaaccggt cgccaacgcg atgttgggca cgcacttctc
    14761 gttcaggtag tcgcggaccg acaggttcgg ctcggtgccg atgtcgccga ccagcgcgaa
    14821 caccttgtcc tgctcgacga gcttcttgac cagcgccggg gtcttggccg ggtcgtaggc
    14881 gtcgtcctcc ttgatcagct cgagcttgta ggacttgccg tccttggcct tcagcccgcc
    14941 gctctggttc acgtaggtga cccaggcgtt gagtccggtg gtcacctggt cgtagatcgc
    15001 gtaggtgccg ctggccggac ggacggtgcc gatcttgatc gtgtcgccgt tgatgccctc
    15061 ggtgccctgg tagtcggtgc agtcgaccga cgggtcgatg aaggcggcgg aactgctgtc
    15121 gccctccttg gccgtggtcg tggacccgga gccggagtcg tcatctcgac ccccgcaggc
    15181 ggtggcggcc aacgtgacga cggccagcaa cgccgccgtc gtcgccagac ccacccgtcg
    15241 agttcgatgt ctcatcatct gtcctcctgg ttggtgatca gaccgtatcg ccggacgcgg
    15301 cggccgcggt ggccgcccgg gcccgcttgg ccgcacgctt ggcggccgac gtccgccaca
    15361 tccccacgat cccgcccggg gcgaacagca tcagcaggat gagcagcacg ccgaggatca
    15421 ccggcgtcgc cggcttgaac cgctcgggca gctccggcga caggacgtcg atgaacaggc
    15481 cgtaggcgac cgcaccgatg gccgggccga tcacgctggc ggccccgccg accaccaccg
    15541 cgatcaggaa gtagatcgac accacgatcg tgaagctcga cgggttgatc cggccgttgt
    15601 tgagggcgaa caccgctccg ccgatgccgg ccatggccga gctgaccccg aaggtgagga
    15661 tcttgacccg ggcgacgtgg acgcccgaga cctcggcggc ggtctcgttg tcgcgcaccg
    15721 ccaccaacga gcgcccggtg cgggaccgga cgatgttgcg catgaccacg aacaccacga
    15781 cggtcagcac caggaacacg aagtacttcc actggtcagg agccagggag ccgcccggtg
    15841 actcgaggcg gatcggtcgc tcgatcacac cgcggggcgt ctcctgcggc gtcgtgatcg
    15901 acaggccgga cccacctccg gtgatcgacg agaactgctc gatgatcagc gggaacagcg
    15961 tcgccaccgc cagggtgacc agcgccaggt acaggccctt gatccgcagc gcggggagac
    16021 cgatggcgac gcccaccacg aagcagatca ccccggctgc ggccaccgac ggccacaccg
    16081 gccagccgag gtcggtgatc aggagcgcgg tcgcgtaggc gccggtcccg tacaacgcac
    16141 cgtgaccgac ggagatctgc ccgttgtagc cggtgaggag gttgagcccg cacgccgcga
    16201 tggccagcgc catccagccg acgaactgct cgacccggaa cgccgggaac atgaacggca
    16261 gggccacgag caccgcagcg accaccaccg acaccaccac gcgccccacc gggacgcggc
    16321 gcgccggtga cggagcgacc ttcgccgtcg ggccgtcgcc gagagccgtt gcgtcgctca
    16381 tacccgctgc acctgctttc gaccgaacag cccttcgggt ctgaacagca ggaccgccag
    16441 gatcaggatg aacgccggcg ccagcggcat cttctcgaac ccgggcacgt actggggcac
    16501 caccgacgag gcgatcccga cgacgaagcc gccgacgacc gcgccgagca ggctgtcgaa
    16561 tccgccgagg gtgatggcgg cgaagccgaa gatcaacgtc agctgcatca tgttggaacc
    16621 gaggccgcgg cccggtgcgg tgaacacccc cgccacggtg ccgaccgcgg cggcgaggcc
    16681 ccacccgagc atgaggagct tggggacggg cacgccgacc agagacgcgg attcggagtt
    16741 cgacgcgacc gcccgcatgg ccaggcccag cccggtgcgc tggaacagca ggtagaagcc
    16801 gagggcgagg accgccagca cgatgacggc gccgatcttc tgccaggcga tggcgactcc
    16861 cgcgacctcg atgccgccgc tgccgaacaa cggtggcagg tcccggtcga gcgtgcccca
    16921 cacgagctgg gcggcggagt tgagacccac gaacaggccg atggtgacca tcacgaccgc
    16981 caacggcttc tcgtggggat cgccgacggg acggaccgcg atctggtgcg cgagcgcgcc
    17041 gacgccgaag ccgccgacca tcccggcgat caccgcgagc ggcaggggca cacccaggtc
    17101 ccagacggtc cacacgatga acgtcgagaa catcgccatc tcgccctggg cgaagttgag
    17161 caggccggtc gaccggtaga tcaccaccag cccgaggccg agcatggcgt agatggcgcc
    17221 gtcgccgatc ccgacgaaca ggacctggag gaagtcggtc atgtcatcag atcccgaggt
    17281 aggccttctg gacggtctcg tcgtgctgca gctgcgcagc cgggccgctg gcgacgatct
    17341 caccggcctc cagcacgtag gcccgaccgg cgaagtcgag ggccaggttg gcgttctgct
    17401 ccacgagcag cacggcggtc ccggtgcggt cggtgatgtc gcgcagccgg tcgaacacct
    17461 cggcggtcac cagcggggcc aggcccaggg acggctcgtc gaggagcagg agccgagggc
    17521 gcgacatgaa cgcccgggcg atcgccagca tctgctgttc gccgcccgac atcgatccgg
    17581 cgagctgatc gcgccgctcc cccagccggg ggaaggcgtc gcaccagagg tcgatgtcgg
    17641 ccgcgacctc ggagtcccgg cgtgtccagg caccgatgcg caggttctcg accacggtca
    17701 ggtcgttgat cgtgccccgt ccctggggga cgtgcgcgat gccggcctgg accacctggt
    17761 cgggccgcag gccgcgcagc gaccggccgt cgaagacgat ctcgccgcgg ctctcgagca
    17821 tccccgacac cgcccgcagg gtcgtcgtct tgcccgagcc gttggcgccg agcaccacga
    17881 ccagctcccc ggcgtcgacg tcgaggtcca ggccgaacag cacctcgacg atgccgtagc
    17941 cggcgcgcag gccgcgcacc tcgagcaggc tcacgacgcc acccccggat ttctgaggtc
    18001 gcgattgtga caagggttgt cggtgcggac ccctatatcg gtcatgcggg ggctccgagg
    18061 taggcggcga tgaccttggg gtcctcgcgg acctgggcgg gcaggccctc ggcgatcttg
    18121 cggcccaggt ccatgaccac gaccttgtcc gagatggcca tgaccatcga catgtggtgc
    18181 tcgaccagca gcaccgtcaa cgagaactcg tcacggatct ggcggatcag cgcgccgagc
    18241 tcgtcgacct ctccgtgggt gagcccgctg gccggctcgt ccagcatcag cagcttcggc
    18301 tggccgacca gggcccgcgc gaactcgatg cgcttgagcg tgccgtaggg cagacccgcg
    18361 cacggacgct gggccagggg tgccagcgac agccgctcga gcacgtccat ggcccgggcc
    18421 tcgagcccgc gctcctgggc cggcatgggc ggcagccgca acgccgcggc ccagaagccg
    18481 cctcggctgc gggcgtgtcc gcccaccttg acgttgtcga ggaccgacag gcccggcacc
    18541 agcgccaggt tctggaacgt gcgaccgacg ccgagctcgg cgatgcggtg cgccggcagt
    18601 ccgagcaggt cgtgcccgtc gaagcggacc gagcccgacg tcggctcgta gatgcggctg
    18661 acgacgttga acaacgtggt cttgcccgca ccgttgggac cgatcagcgc gcagatctga
    18721 ccggcgtcga tggtgaacga caacgagtcg agcgcgacca ctccgccgaa ccggaccccc
    18781 acgtcgacca cttcgagcac tgccacggcg tacgtcctcg gtgtccggtt ccgtccccgc
    18841 agggcccgga ccgtccacct ggcccgagtg gcgcggaacc ctagcgtccg ccgagccgca
    18901 ccggttcgat atcgcggccg acgccggatg atcagtcggg ctggggctcc atcgcagcgc
    18961 ctccaccgct gggccgccac cggccacccc gtgaccccgg cccaccgttc agcggcgcgc
    19021 ctccatgaac atgtcgcgga gctcctcgta ggactcgatc acccgtcccg cgccgagcac
    19081 caccgactcg agcggctggc gcaccatgcg caccggcacc tcggtctcct cggccagccg
    19141 caggtcgagg cccttgagca gggacccacc gccgaccagg aagatgcccc gctcgatcag
    19201 gtcctgggcc agctcgggcg gggcctcggc cacgcagctc agcaccgagt cgaccatggt
    19261 gttgaccggc tcggcgatcg cggttcgcac ctcggccgcc gagaggatca ccgtcttggg
    19321 caggccggac atgagctcgc gaccccgcac ctcggcgcgc acctcgtcgg gcgtcgggta
    19381 cgcggagccg atgaggatct tgatctcctc ggcagtctgc tcgccgatgg cgatcccgta
    19441 ctcacgacgg atgtaggtga tgatcgacgc gtcgatgtcg aagcttccga cccgcaccgc
    19501 ctgcaacgcc acgatcccac cgagggagat caacgcggtc tcggaggtgc cgccgccgat
    19561 gtcgacgacc atgttggcca tcggctcgtg gatgtgcagc cccgcgccga tcgcggccgc
    19621 catcggctgc tcgatcagct gcgcctcggt ggccccggcc ttgcgggccg cctcggtgac
    19681 cgcccgcttc tccaccgcgg tgatcgcgga cggcacgcag atgaccactc ggggacggtt
    19741 gaagcggttg acgcccaccc gcttgagcag gagccggatc atctcctggg tgacctcgaa
    19801 gtcggtgatc gccccctggc gcagcggacg caccgccacg atgtagctgg gggtccggcc
    19861 gatcatcttc caggcgtccc ggcccatggc caggacctcg ttggtgcggc tgttgagcgc
    19921 gatcaccgac ggctcgttca acacgatgcc ctcgccgcgc gcgtagacca gggtgttcgc
    19981 cgtccccagg tcgatggcca ggtcgcgtgc cagcgtccta cctccgggtc cggcgtcgac
    20041 ggaccacccg gccctcgatc tcgggctcgt cgggggccag cgcgcgcatc ttgtcgtgga
    20101 actggtcgat gctcgagtgc ggagaggacg gcggccggct ccgcaggacc aggatggtca
    20161 cgccgaccac gctgatgacg agcgcgaccc cgaggaagac caggttgctc acggtctcgt
    20221 cgtccccaag ggatctggac cgacctgatc cgcaccgacc tgatcggcgc cgacgacgac
    20281 gcgggcgtcg gagccccagt cctgcccgcc ggcccagcgc tcgcgcttcc acaccggcac
    20341 cgactccttg accgcgtcga tggcgaagcg ggccgcgtcg aacgcctcgg cccggtgagg
    20401 tgccgacacg gcgacgacga cggcctcctg gccgatcggg acgtcgccga cccggtgaag
    20461 gatcgccacc cgaccggcgg cgggccagcg ccgacgcagc tcgtcgacca cccggtccat
    20521 ccgcgccacc gcgtggtcct cgtaggcctc gtaggccagc tcggtcacct cgggtcgacc
    20581 ggccgagtgg tcccgggcgg tcccggtgaa cacgaccacc gcaccgcagt ccggtcggac
    20641 cgcccacgcg taggcgtcgc ccaccggaag gccaccggcg acgagcgcgg tccagtcgtc
    20701 gccgcgctcg ggcggacgcg acacgctcgg ggttccggac gtcggcacgg cgtcatcgta
    20761 gggaagttcg acgcccgcgc ccatccggag ccgcggccgg cgcggcgtcg acgatcggta
    20821 tcgtcgggct ccatgcagga accggccgag cgcgggcccg acggctccga cgccgcggcc
    20881 gacggagcga ccgcccggcg ttgggtgcac cccagcgagc tgggcctgca gacacgttcg
    20941 cgttcggacc gacggcgcgg cacctggttg gcctcgacgc tggtgctcgg cggcctcggc
    21001 ctgctggcgt tcggtctgat catcggggtc ggggcggacc gggatcccgg caccgttgcg
    21061 tcatccccgt cccacgacgt ctccgcgacg ttggcgtcgc tcaccgtcct cgacggccag
    21121 agccgacgca cggtgaccgg cgtcgtgctc gatgccgagg gccatgtggc ggtccggtcc
    21181 ggttcggtca gcggagccca ggaggtctgg gtgagctgcg gcggcgctgc accccaggcg
    21241 gcccgcgtcg tggcccacga cgacggtctg gacctggcgg tcgtcgcgct cggcgaaccc
    21301 gcgggccgac cggtcaccgc ggccggtgag gcgtcc
//
LOCUS       DAOQOD010000034        21098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_854_length_21098_cov_12.992634, whole genome shotgun sequence.
ACCESSION   DAOQOD010000034 DAOQOD010000000
VERSION     DAOQOD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 21098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21098
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_854_length_21098_cov_12.992634"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLZ72_05290"
     CDS             complement(<1..491)
                     /locus_tag="PLZ72_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA35746.1"
                     /translation="MNAADSTGATVLTGATVLTPERQPDGTAIALLGDRIAAVGTEAD
                     CRAALRGAGVPDDSVTTEDLTGSTVVPGFVDAHLHPMVMAVFEQQVLLDQVRSIAELN
                     DLLADRARSTPPDETIVGFQLDGEVLAEGRVPTAAELDAATGNRAVVLLCRDGHHAVG
                     STVA"
     gene            complement(488..1672)
                     /locus_tag="PLZ72_05295"
     CDS             complement(488..1672)
                     /locus_tag="PLZ72_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNA35747.1"
                     /translation="MSDSHRSAPVRTVELFCAEVPLSRLARSAMSHSDSGLGMAIAAE
                     DPWEVAEFLFVRLTDEDGVEGWGECYVWTPESAVSHRGMAMVIEEHLGRYLLDARPTD
                     VQAIGARMDRNIPRNEMAKGVLDMALHELAARQVDRPVHDLIGGAAVDRIPLCALVPM
                     ADPDFVAKICSGYVDAGYRSLRIKLGAGPRADLEVMTAVRDAVGWDVRIRCDFNQAYD
                     APTAVRVLRLLEPLDLDAAEQPLPVGDLIGMRWVQERTAVPLFLHEGFFQMSELVTAV
                     ELGAMGVSGINAERPGGITAALRAIDYAAARGMGTIIHNQPLGIGTAAHAHVAAARWD
                     RLGHAPELAGDVMFEEHLTDARYPMEDGCLVLPGGAGWGITVDRDALERHLVAEPVVL
                     SA"
     gene            1658..2587
                     /locus_tag="PLZ72_05300"
     CDS             1658..2587
                     /locus_tag="PLZ72_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNA35748.1"
                     /translation="MGVAHAGNVTVASPLCIIDYIPRSDRPPTVASAAIPDRVAAATL
                     SPALRRIAEVLIDDPQAIAFGTVGTVAERAGTSTPSVVRLATALGYEGFADLRDAARD
                     ELAARIRTDADRVARPAPEDPVVALIEVERANVTDTLAGVDPAAVTAVVDLLSDRSRR
                     VWVLPSTQTEGVARRLVDQIDIVRGRAALLGGTEFRIASTLQALRPGDLVVTMDVPRH
                     EAALVRLQAAAVDRGGVPVVLSGDTRTALRFEGGHLLAFARDSVGPFDSLVGLTVLCT
                     LLVNSLVERHKADASRRVVELEQTWTDRDLFRI"
     gene            complement(2648..3652)
                     /locus_tag="PLZ72_05305"
     CDS             complement(2648..3652)
                     /locus_tag="PLZ72_05305"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNA35749.1"
                     /translation="MATDSTRRPQSSPAVWRAADVADPTTWTFELTDDHRREIVAAAR
                     SASTSATPSVTESASFPLPTLGPEIARWTAELAHGRAFQLVRGFPVDELDPDETRTAF
                     LGLGSHLGVPVGQNRSGEILTDIRDERLPAEEPHVRRYRTRLRQDFHTDGADIVGLLC
                     LHTALRGGESRIASAGAVYNALLESRPDLLEVLHRPFHWDRQGEEAPGEQPWFELPPL
                     SEVDGTPRFFYLGWYIRDAQRHPEVPRLTDEQLEAMELLEVIANDTAVHVEMDFRPGD
                     IQWINNGRVLHAREAYDDAEDPAERRHLLRLWLAAHDFASVEGELRAGIGPDAPVTPS
                     "
     gene            complement(3656..4174)
                     /locus_tag="PLZ72_05310"
     CDS             complement(3656..4174)
                     /locus_tag="PLZ72_05310"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNA35750.1"
                     /translation="MHRTTSIDPQEVTMSRIQLAINVTDVDSAVSFYERLFGTPAAKR
                     KDGYANFEIAEPPLKLVLIESAEGGTLNHLGVETTTAAEVAAAEARLSGAGLQTTGVD
                     DTTCCYATKTETWVTDPDGAKWEWYVKTGDAEQMSPSAADSATCCAPAAVPESLTEVE
                     TASTGASSVSCC"
     gene            complement(4183..4710)
                     /locus_tag="PLZ72_05315"
     CDS             complement(4183..4710)
                     /locus_tag="PLZ72_05315"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNA35751.1"
                     /translation="MGDHRPQVLFVCVHNAGRSQMAAALLAHHGGDRVVVRSAGTAPA
                     EEINEAVVEVMAEVGIDLRSSGAHPKLLDPAAVRVSDVVITMGCGDECPFHPGVRYLD
                     WVLDDPAGLRVDEVRPIRDEIDRRVRALLVDLVGEETSGVPIIPGHRPRLLRRLGGAG
                     RSGPRKCIDNYQCIV"
     gene            complement(4722..5459)
                     /locus_tag="PLZ72_05320"
     CDS             complement(4722..5459)
                     /locus_tag="PLZ72_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNA35752.1"
                     /translation="MSERGGAAEDLLAVAPETGTGELRLDLSRRLVAEGLGTGLLVVA
                     VVGSGIAASRLSAGDVGLQLLENSLATGAALVGLILAFGAVSGAHFNPVVTMVDRLLG
                     TATTRDSLLYVVAQFVGGCAGAVVANIMFELPAVQLATAERSSPALWLSEIVATLGLL
                     LVIQGCVRSGRSDVVAFAVAGWIVGAYWFTSSTSFANPAVTVARALSDTFAGIAPSSV
                     PMFLVMQILGAAIAAGLIRLFYPHPPS"
     gene            complement(5456..6118)
                     /locus_tag="PLZ72_05325"
     CDS             complement(5456..6118)
                     /locus_tag="PLZ72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA35753.1"
                     /translation="MVDRTERVRRLAALGERVRLAIVDELASGDRSPGELRRRLELES
                     NLLAHHLDVLEGAGIVTRTRSDGDGRRCYVRLNRPALVGLLPAPDGVVGEALFVCTHN
                     SARSQLAAATWRSVVGTRAWSAGTHPAERVHPGAVAAAERGGLDLDGSVPRSLDDVEL
                     PPLVITVCDRAHEELSEHTGWLHWSIADPVPSGDAAAFDRVVAELHDRVSAMLSDQPG
                     AG"
     gene            6244..>7136
                     /locus_tag="PLZ72_05330"
     CDS             6244..>7136
                     /locus_tag="PLZ72_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA35754.1"
                     /translation="MPDISPREPLSRNVRVVSLVSFLQDTASELLYPVLPFFVTGVLG
                     ASPAVLGLIEGLADGTASVVKAASGRLADLRHRRPLVATGYGLSALGKVLLALATAWP
                     AVLGSRFTDRVGKGMRGPPRDAIIADETTPQNRGRAFGFHRAADTAGAVVGPLIGLFL
                     YDLVGQRFRPLFWIAVIPAVLSVALVFAVRERPHPHARQERAWSLHGMPNRFWRVLAI
                     VGLFGLVNFPDTLLLLRAKDLGFDFAGVVALYVLYNLTYAAGSYPAGRLSDRWSRSVV
                     VAAGLAVFAVAYLGFGLTSTSA"
     assembly_gap    7137..7236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7237..7574
                     /locus_tag="PLZ72_05335"
     CDS             <7237..7574
                     /locus_tag="PLZ72_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35755.1"
                     /translation="LAVFAVAYLGFGLTSTSAWAWVLFPLYGCYTALTDGVIRAWIAD
                     LVPAHSRGTALGVQAAVSGFGLLVAGVWAGLAWGGTGRVPFLISGLLVAGLALLLFAL
                     GPSLTSPPP"
     gene            7728..7931
                     /locus_tag="PLZ72_05340"
     CDS             7728..7931
                     /locus_tag="PLZ72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNA35756.1"
                     /translation="MAMTLRLDQADQDALREHAAAEGISMQDVARRAIRDYIARSNHR
                     ARVSNATELILNVHADAIERLGQ"
     gene            7928..8320
                     /locus_tag="PLZ72_05345"
     CDS             7928..8320
                     /locus_tag="PLZ72_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNA35757.1"
                     /translation="MSEPVEFLDLDDLVDMARILLGDPPPIRDIGLLGSAAARPQTTV
                     GGRDAYPTVWSKAAALLQSVVDNHALVDGNKRLGWLATAVFLEINDASVAGATDDEVF
                     DLVWAIASARSTIEEIAHHLEMMSRRRS"
     gene            8589..9149
                     /locus_tag="PLZ72_05350"
     CDS             8589..9149
                     /locus_tag="PLZ72_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA35758.1"
                     /translation="MLIITGPPGAGKSTVAASVAQHSATAVRVEADWFWTTIVARFID
                     PWLPEADAQNRTVLRSAFHAARAFEDGGYDTVVEGIVGPWMLDVVADTLRDSSPVDYV
                     VLRPSLTSCLSRAARRPAVERVPGHPPLDRSGPIRHMWARFSDLGRLESHAVDTSTMS
                     EDETVRAVLEARHDGRLRVELPTPRS"
     gene            9290..10084
                     /locus_tag="PLZ72_05355"
     CDS             9290..10084
                     /locus_tag="PLZ72_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35759.1"
                     /translation="MGPLTDRRYLRDSQYRETANLVARSNLHERYGRGDWFRWIASQA
                     PWPDHGWVLEVGCGTGRLWADAADIVPDSSRVVLCDLSPTMVRESLAVADGATGGSVA
                     ALPQLPFPDDSFDLVIVSMVMQHLDVPEPAIAELARVTAPRGALVIGAASEQHLDEIM
                     VLRRRFGDDDATIGGAFGAGGLDAATERVARSFSRVDWQRYEDQLVVDDREAIVDYLR
                     SWSPLDDADDDTLQRLRAEVDRQFDAEFGVMTITKDVGCIVARSPS"
     gene            10151..11185
                     /locus_tag="PLZ72_05360"
     CDS             10151..11185
                     /locus_tag="PLZ72_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA35760.1"
                     /translation="MASRGVLRCLAAAVLFGASAPAASVLAGDMTALVLAGLMYLGAA
                     LAVAPLCVRRRPRPEVLRAGWRPLGLAVVFGGALGPALLVAGLAQVPAATASLMLNLE
                     LVATVALAATVFREHLGRRLLAAAALLTIAGVLLVLEPGVGVQPGALLIVGACACWGI
                     DNAVTARIDQISPEQVTLVKGIVAGSCNVVIGLVVAGGWGVSTSELAAALAIGAVGYG
                     ASITLWVKGAQDLGAARGQVIFATAPFIGALISWTVLSEPVTATQAIAVPIAAVGVVL
                     SLRSSHEHEHRHRVTVHEHEHTHDDGHHEHSHEDGFAGRHTHRHEHRDLVHAHPHVPD
                     LHHRHDHGID"
     gene            11252..11572
                     /locus_tag="PLZ72_05365"
     CDS             11252..11572
                     /locus_tag="PLZ72_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35761.1"
                     /translation="MCELGLDVDLMLLSADEVASELGARVRRERLRQNMTQRTLADRA
                     GVSRATLARMEQAGTATLPNFLAVLAALRRTGDLQQLLEPPPTETIGHFLARSEPQRL
                     RGRR"
     gene            11569..12837
                     /locus_tag="PLZ72_05370"
     CDS             11569..12837
                     /locus_tag="PLZ72_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNA35762.1"
                     /translation="MRAVDVVYEGADGPWIVARVAQRGSETFVEYTDEHVATGADLAP
                     LKHPVDQRSRSFPGATPEHLPGLLSDAGPDSWGRSVLRRDMRRHGIDAPEPLDMLTWL
                     GRRTMGALTFRPVEGPEPADVALVELDRLQDEALRHLAGQDTHDQAAEAVALAAGGSA
                     GGARPKITAARSPEGELIADNGDLPPGSTPWLVKFHGQDDSASLSVVEASYLEMARTA
                     GISTCEHELLPGSSGTQYLAVRRFDRTVEPGAQPGRVHMATAAGLLEAYPELGQHVGY
                     HDVIRLTRRVTGDVRETTEMVRRAVFNVVAHNRDDHARNIAYLWTPADGWRLAPAFDL
                     TFSLGPRPTFAAAGPGEHYLDVAGKGASITRDDLVGLHKPAGLEPHVVADMVDEAVAA
                     VDEWPTVADRNGVDADTIEHAAARLPALGL"
     gene            12996..13199
                     /locus_tag="PLZ72_05375"
     CDS             12996..13199
                     /locus_tag="PLZ72_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35763.1"
                     /translation="MLAVIYMVSRYWIATLVSPEETSAQVRVVFDQALVDQGGLLTDD
                     PARLILLAVSDNGPAKTVVRHTC"
     gene            complement(13269..13598)
                     /locus_tag="PLZ72_05380"
     CDS             complement(13269..13598)
                     /locus_tag="PLZ72_05380"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNA35764.1"
                     /translation="MTDIKHYAYRVLWSAEDDEFVATVAEFPSLSWLHADQTKALQGL
                     VDLVAGVVADMEASGDPIPEPITERRFSGKFNVRVPESLHRELALSAAQEGVSLNRLV
                     SDRLARS"
     gene            complement(13595..13849)
                     /locus_tag="PLZ72_05385"
     CDS             complement(13595..13849)
                     /locus_tag="PLZ72_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35765.1"
                     /translation="MAKIDKTVAAMRNAPQNNSYNELRSVCVHFFGEPRQSGASHAAF
                     KTPWPGDPRVNIQKAKDGKAKSYQVRQVLKAIDKVQEEQK"
     gene            complement(14131..14251)
                     /locus_tag="PLZ72_05390"
     tRNA            complement(14131..14251)
                     /locus_tag="PLZ72_05390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14178..14180),aa:Leu,seq:caa)
     gene            complement(14365..14631)
                     /locus_tag="PLZ72_05395"
     CDS             complement(14365..14631)
                     /locus_tag="PLZ72_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35766.1"
                     /translation="MTAFRELVHRLRSDAGLTQAELADRMGTTESAIARMEGGGTRPT
                     LETLEKVAAAVGADLVVGVGENLSEHRTIVKLEREGHAVVRRAG"
     gene            complement(14639..14836)
                     /locus_tag="PLZ72_05400"
     CDS             complement(14639..14836)
                     /locus_tag="PLZ72_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35767.1"
                     /translation="MRSTFAEFSLAVLEHWEQVAGLDLPPPRGVGSVVFNHDQFPAAL
                     RIRELSSKLTAQLVDEQRCGG"
     gene            15319..15942
                     /locus_tag="PLZ72_05405"
     CDS             15319..15942
                     /locus_tag="PLZ72_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNA35768.1"
                     /translation="MDFVRPIEAIVPGAQGRVLAVLAETTAELNLRSIAQLAEISQAQ
                     TSRLLPELVDLGVVERREVPPASLFRIVPEHIASRALLALARSADAVLDEMGRLARAL
                     PHPPASVIVFGSFARREADTHSDIDVVIVRPTEIDEDDDEWLDSLDTWRRAVRRLSGN
                     VVEVLDVSTDEATAKLRGRSQVWTDIRRGGQVVHGLSIDELRDARSG"
     gene            15899..16342
                     /locus_tag="PLZ72_05410"
     CDS             15899..16342
                     /locus_tag="PLZ72_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35769.1"
                     /translation="MASASTNSGTPEVARPARTRSVSAAQVRSYAAKAREFAEAAASD
                     LQAGRNIAATSLAIHAGINAADAVCGARLAKRAAGEDHDQVLGLLGQAGPDGAEVARD
                     LRRLLPLKTKAEYEPDDIAPSIAAKAVERARRCAAVAQRVAERRG"
     gene            16852..17085
                     /locus_tag="PLZ72_05415"
     CDS             16852..17085
                     /locus_tag="PLZ72_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNA35770.1"
                     /translation="MFARGRPPALARPSSPRAGSGRTAGTDAGKAWNDADAEALIDED
                     PRSYKDIDQVMDDQQDLVAVHHTLSQILNYMGV"
     gene            complement(17173..18135)
                     /locus_tag="PLZ72_05420"
     CDS             complement(17173..18135)
                     /locus_tag="PLZ72_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35771.1"
                     /translation="MQVQDGSRLVGRVFPAVDSYGSGSSVGWQALLVVDGDPVEVWNR
                     YAEVLRVKDGDAPESCVVRTVTVPGDPSATSTTALVTVGDGTPSRFLTEPRLDGENRL
                     DCDFTDGPLAMSMAVGAAVQCDSLTPASGCRLRPESHLFIRSSPGTTTSSTDLPRRGT
                     DQLRYERGLASVAGTDKVNVPPTAAIPTGAVVAANLPTNLDPTRLPGPGDPIDDGIDE
                     YLVGSPFKVPAGGRSLVAPSQLIDCNSGLVAVVSVPGKPEATVAWFDSSSTGDDPIQT
                     KTGTDSGGTPWVNGLISTAGGYYLDITAVPAGDRTDALVTECGD"
     gene            complement(18417..18875)
                     /locus_tag="PLZ72_05425"
     CDS             complement(18417..18875)
                     /locus_tag="PLZ72_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35772.1"
                     /translation="MQRARTEAEKETRAAEHRLEFERRRFERLGQRRNGPRDGRPVRV
                     DVDDDAWAVLQRDVVAQGTYLIWRMGELVRFEMDQLAAGNLSGRPSSRRRRGPGEGEP
                     SPRQRFLRIQVDDARWKDLRLAAVDLGVSSARYLGEIAEAEAHRLGWRAL"
     gene            complement(19225..19758)
                     /locus_tag="PLZ72_05430"
     CDS             complement(19225..19758)
                     /locus_tag="PLZ72_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35773.1"
                     /translation="MTELLAYVDESERPGRYLMSCVVIDRADAGRARSAARGLLLPGQ
                     RRLHFHSESDRRQRFLVADLLAIDVGASVFVCRSAPGRRTAQARAACLAAIVLDLQST
                     GEPVRLTLESRHNQDADDHPIIWAARRPTPPLTYEHVSGAGEPLLWLADAYAWLVGAG
                     GDWQRRLGNSVRVIDVG"
     gene            complement(19755..20900)
                     /locus_tag="PLZ72_05435"
     CDS             complement(19755..20900)
                     /locus_tag="PLZ72_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA35774.1"
                     /translation="MRGHVHKRGSTWGYVVDVGRDPATGKRKQRTKGGFRTRAAAEDA
                     LTEVLNEIRSGTVVAPSSVTLEEYLTEWLTTVRPQLRETTWASYGVAVQRICWGLGAG
                     KLQALTAVEVERFYARLVNEGGPAGRPLAAKTVRNTHIVLHRALADAERLRLVMRNVA
                     HLARPPAVPHVERATWTADELAAFLEHVSGDRLFAAYVLLATTGMRRGEVFGLRWRDV
                     DLSARRASVTQTVTTVNDKLVLGPTKTNRSRRTISLDPTTVDVLHAHRVVQDQERATA
                     GQAWDPSYDLVLCDGTGRPEHPDRFSRQFQRYVKATDLPPLRGPHNLRHTWATLALRA
                     GVHPKVVSDRLGHATIAVTIDTYSHVAPSLDAEAADTVAADIFGSSA"
     gene            complement(21004..21087)
                     /locus_tag="PLZ72_05440"
     tRNA            complement(21004..21087)
                     /locus_tag="PLZ72_05440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21051..21053),aa:Leu,seq:taa)
BASE COUNT         3170 a         7433 c         7184 g         3211 t
ORIGIN      
        1 gcgacggtcg aaccgacggc gtggtggccg tcgcggcaca ggagcaccac cgcacgattc
       61 ccggtggcgg cgtcgagctc ggcggcggtg ggcacgcgtc cctcggcgag cacctcgccg
      121 tcgagctgga acccgacgat cgtctcgtcc ggcggggtgc tgcgggcccg atccgcgagc
      181 aggtcgttca gctcggcgat cgatcggacc tggtccaaca ggacctgctg ttcgaacacg
      241 gccatgacca tcgggtgcag gtgggcgtcg acgaacccgg gcacgaccgt ggagccggtc
      301 aggtcctcgg tcgtcacgct gtcgtcgggg actccggcgc cgcgcagggc ggcccggcag
      361 tccgcctcgg tgccgacggc ggcgatccgg tcgccgagca gcgcgatcgc ggttccatcg
      421 ggctggcgct ccggggtcag gacggtggcg ccggtcagga cggtggcgcc ggtggagtca
      481 gcggcgttca cgccgagagc accaccggct cggcgaccag gtgacgctcg agggcgtcgc
      541 ggtcgacggt gatcccccac ccggcgccgc cgggcaggac caggcaaccg tcctccatgg
      601 gatagcgggc gtcggtgagg tgctcctcga acatgacgtc accggccagc tcgggtgcgt
      661 ggccgaggcg gtcccagcgg gccgcggcga cgtgggcgtg ggccgcggtg ccgatgccca
      721 gcggttggtt gtggatgatc gtgcccatgc cgcgggccgc ggcgtagtcg atggcccgca
      781 acgccgcggt gatgccgccc ggccgctcgg cgttgatgcc gctcacgccc atcgcgccga
      841 gctcgacggc ggtcacgagc tcggacatct ggaagaagcc ctcgtgcagg aacagcggca
      901 ccgcggtgcg ctcctgcacc caccgcatcc cgatcaggtc ccccaccggc aggggctgct
      961 cggcggcgtc gaggtcgagc ggttcgagaa ggcgcaggac ccgcaccgcg gtgggagcgt
     1021 cgtaggcctg gttgaagtcg cagcggatgc ggacatccca gccgaccgcg tcgcgcaccg
     1081 cggtcatcac ctcgaggtcc gccctcgggc cggcgccgag cttgatgcgc agcgaccggt
     1141 acccggcgtc gacgtagccc gagcagatct tcgccacgaa gtccgggtcg gccatgggga
     1201 ccagggcgca cagcgggatg cggtcgaccg ccgctccgcc gatgaggtcg tgcaccggcc
     1261 ggtcgacctg tcgggccgcc aactcgtgca gggccatgtc gagcacgccc ttggccatct
     1321 cgttgcgggg gatgttgcgg tccatgcggg cgccgatggc ctgcacgtcg gtcgggcggg
     1381 cgtcgagcag gtagcgcccg aggtgctcct cgatgaccat ggccatgccc cggtgcgaca
     1441 cggccgactc gggcgtccac acgtagcact cgccccaccc ctcgacgccg tcctcgtcgg
     1501 tcaggcgcac gaagaggaac tcggccacct cccacgggtc ctccgccgcg atcgccatgc
     1561 ccaggccgga gtcgctgtgc gacatcgcgg atcgcgccag ccgcgacagc ggcacctcgg
     1621 cgcagaacag ctcgacggtg cggacggggg ccgatcggtg ggagtcgctc acgccgggaa
     1681 tgtaacagtt gcttcaccgc tctgtatcat cgattacatc ccacgctcgg accgaccccc
     1741 caccgtcgca tcggcggcga tcccggaccg ggtcgccgcc gcgaccctgt ccccggcgct
     1801 gcggcgcatc gccgaggtgc tcatcgacga cccacaggcg atcgcgttcg gcaccgtcgg
     1861 caccgtcgcc gagcgcgccg ggacgagcac cccgtcggtg gtccggctcg ccaccgctct
     1921 cggctacgag ggcttcgccg acctgcgcga cgcggcccgc gacgagctcg cggcgcgcat
     1981 ccgcaccgac gccgaccggg tggcccgtcc cgcccccgag gacccggtcg tcgccctgat
     2041 cgaggtcgag cgggccaacg tgaccgacac cctcgccggc gtcgatcccg ccgcggtcac
     2101 cgccgtggtc gacctgctgt cggaccggtc ccgtcgggtc tgggtgctgc cgtcgacgca
     2161 gaccgagggc gtggcccggc gcctggtcga ccagatcgac atcgtccgcg gccgcgccgc
     2221 cctgctgggc ggcacggagt tccggatcgc ctcgaccctc caggcgctac ggcccggcga
     2281 cctggtggtg accatggacg tcccccgcca cgaggccgct ctcgtccgcc tccaggccgc
     2341 tgctgtcgac cggggtggcg tgcccgtcgt gctgagcggc gacacccgca ccgccctccg
     2401 cttcgagggc gggcacctgc tcgccttcgc ccgggactcg gtcgggccgt tcgactcgct
     2461 cgtcgggttg acggtgctgt gcaccctgtt ggtcaactcg ctcgtcgaac gacacaaggc
     2521 cgacgcgtcc cgacgggtgg tcgagctcga gcagacctgg accgaccggg acctgttccg
     2581 gatctgaggt cccgcagggt gcggagcagt ggtcggggcc ggcgaccggc agccccgagc
     2641 cccccggtca gctcggcgtc acgggagcgt cgggcccgat gccggcccgc agctcgccct
     2701 cgacgctggc gaagtcgtgg gcggcgagcc acaaccgcag gaggtggcgt cgctccgcgg
     2761 ggtcctcggc gtcgtcgtag gcctcgcgtg cgtgcagcac ccggccgttg ttgatccact
     2821 ggatgtcgcc gggccggaag tccatctcga cgtgcaccgc cgtgtcgttg gcgatcacct
     2881 cgagcagctc catcgcctcg agctgctcgt cggtcaggcg cggcacctcg gggtggcgtt
     2941 gcgcgtcgcg gatgtaccac cccaggtaga agaagcgcgg cgtgccgtcg acctcgctga
     3001 gcggcggcag ctcgaaccac ggctgctccc ccggcgcctc ctcgccctgg cggtcccagt
     3061 ggaacggacg gtgcagcacc tcgagcaggt cgggccgcga ctcgagcaac gcgttgtaga
     3121 ccgcgcccgc gctggcgatg cggctctcac cgccccgcag cgccgtgtgc aggcacaaga
     3181 ggccgacgat gtcggctccg tcggtgtgga agtcctgtcg cagtcgggtg cggtagcgcc
     3241 ggacgtgcgg ctcctcggcc gggagccgct cgtctcggat gtcggtcagg atctcaccgg
     3301 agcggttctg gccgaccggg acgcccaggt gggagcccag tccgaggaac gcggtgcggg
     3361 tctcgtccgg gtcgagctcg tccaccggga acccgcggac gagttggaac gcccggccgt
     3421 gcgcgagctc ggcggtccag cgggcgatct ccggtccgag cgtcggcagc gggaacgacg
     3481 ccgactccgt cacgctcggg gtcgccgagg tcgatgcgga gcgggccgcg gcgacgatct
     3541 cacgtcggtg gtcgtcggtc agctcgaagg tccaggtggt cgggtcggcc acgtcggccg
     3601 cccgccacac cgccggcgag ctctggggtc gtcgagtgga atcggtcgcc acggttcagc
     3661 agcagctgac cgacgaggcg ccggtcgacg ccgtctccac ctcggtcagg ctctccggaa
     3721 cggcggccgg agcgcagcac gtggcggagt cggcggcgct gggagacatc tgctcggcgt
     3781 cgccggtctt gacgtaccac tcccacttgg ccccgtcggg gtcggtcacc caggtctcgg
     3841 tcttggtggc gtagcagcag gtggtgtcgt cgacgccggt ggtctggagt ccggcgcccg
     3901 acaggcgggc ctcggccgcg gcgacctcgg ccgcggtggt cgtctcgacg ccgaggtggt
     3961 tgagggtgcc tccctcggcg gactcgatga gcacgagctt gaggggcggc tctgcgatct
     4021 cgaagttggc gtagccgtcc ttgcgcttgg cggcgggggt gccgaagagc ctctcgtaga
     4081 acgagacggc ggagtcgacg tcggtgacgt tgattgcgag ctggatccgg gacatggtga
     4141 cctcctgggg atcgatcgat gttgttcgat gcatcggtca ccctacacga tgcattgata
     4201 attgtcgatg cattttcgcg gacccgagcg acccgcacca ccgagtcgtc gcagcaggcg
     4261 cggtcgatgc cccgggatga tcggcacccc tgaggtctcc tcgcccacca ggtccacgag
     4321 gagggcccgg acccgtcggt cgatctcgtc gcggatgggg cggacctcat cgacgcggag
     4381 gccggccggg tcatcgagca cccaatcgag atagcgaacg cccggatgga acgggcactc
     4441 gtcgccgcag cccatcgtga tgaccacgtc ggagacccgg accgcggccg ggtcgaggag
     4501 cttcgggtgc gctccactcg agcggaggtc gatccccacc tcagccatga cctcgacgac
     4561 cgcttcgttg atctcctcag cgggtgcggt accggccgag cggaccacga cgcgatcacc
     4621 gccatggtgg gcgagcagcg ccgcggccat ctgggaccga ccggcgttgt gcacgcagac
     4681 gaacaggacc tggggtcggt ggtcacccat ggggtgccgg atcagctcgg gggatgcggg
     4741 tagaagagtc ggatcaggcc cgcggcgatc gcggcgccga ggatctgcat gaccaggaac
     4801 atcggcaccg acgacggagc gatgccggcg aacgtgtccg agagcgcccg agcgaccgtg
     4861 accgccgggt tggcgaagct cgtggagctg gtgaaccagt acgcgccgac gatccacccg
     4921 gcgaccgcga acgccaccac gtcggaacgg ccggatcgca cgcagccctg gatcaccagc
     4981 agcaggccga gggtcgccac gatctcggac aaccacaggg cgggcgagga ccgctccgcg
     5041 gtggcgagct ggacggccgg cagctcgaac atgatgttcg ccaccacggc tcctgcgcag
     5101 ccgccgacga actgcgccac gacgtacagg agcgaatccc gcgtggtggc cgtgccgagg
     5161 agccggtcca ccatggtgac gacggggttg aagtgggcgc cggacaccgc accgaacgcg
     5221 aggatcagac cgaccagggc tgctccggtc gccagggagt tctcgagcag ctgcaggccc
     5281 acgtcacccg ccgacagcct ggacgccgcg atccccgacc cgacgacggc gacgaccagg
     5341 agtccggtcc cgagtccctc ggcgacgagt cgacgactca gatcgagccg cagctcaccg
     5401 gtgccggtct cgggagcgac ggccagcagg tcctccgcgg ctcccccgcg ttcgctcacc
     5461 cggcacccgg ctgatcggac agcatcgctg agaccctgtc gtgcaactcg gcgaccacac
     5521 gatcgaacgc ggccgcgtct ccggagggga ccgggtcagc gatcgaccag tgcaaccacc
     5581 cggtgtgctc cgacagctcc tcgtgagcgc gatcgcacac cgtgatgacg agcggcggca
     5641 gctccacgtc gtcgagcgag cggggaaccg agccgtccag gtcgagccct ccccgttccg
     5701 ccgccgcgac cgctccgggg tggacccgct cggccggatg cgtgccagcc gaccacgctc
     5761 tggttccgac cacggaccgc caggtcgccg ccgcgagctg gctgcgcgcc gagttgtggg
     5821 tgcacacgaa gagcgcctca cccacgaccc cgtccggggc cggcagcagg ccgaccagcg
     5881 ccgggcggtt cagccgcacg tagcagcgac gtccgtcgcc gtcggaccgg gtccgtgtga
     5941 cgatccccgc cccctcgagc acgtcgaggt ggtgggcgag caggttcgac tccagctcga
     6001 gacgacgacg cagctcgccg ggagaacgat ccccgctggc cagctcatcc acgatcgcaa
     6061 gccggacccg ctcgcccagc gctgcaaggc gcctgacgcg ttccgttcgg tcgaccatcg
     6121 gagagcagcg tagtctctcg atagatcttg agtcatcctc tgtcgaggtt tgcccggacc
     6181 gtgcgtcccg cacagcggct cccactcgct ccggacgaac cccgcatgtg ggagcatcag
     6241 cgcgtgcccg acatctctcc cagggagccg ttgagccgca acgtcagggt cgtctcgctc
     6301 gtgtcgttcc tgcaggacac ggcgtcggag ttgctgtacc cggtcctgcc gttcttcgtg
     6361 accggcgtgc tgggggcgtc tccggcggtg ctggggttga tcgaggggtt ggccgacggc
     6421 accgcatcgg tggtcaaggc ggcgtcgggt cgcctggcgg acctgcgtca ccgtcgaccg
     6481 ctggtggcca cggggtacgg cctgtccgcg ttgggaaagg tgctgctggc gctcgcgacc
     6541 gcgtggccgg cggtgctcgg gtccaggttc accgaccggg tcggcaaggg catgcgaggc
     6601 cctccccgcg atgcgatcat cgctgacgag accacccctc agaaccgcgg ccgcgccttc
     6661 gggttccacc gtgcggccga cacggccggc gcggtggtcg gcccgctgat cggactcttc
     6721 ctctacgacc tggtcggcca gaggttccgg cccctgttct ggatcgcggt gatccccgcg
     6781 gtgctgagcg tcgctctcgt cttcgccgtg cgagaacgac cgcaccctca cgcccgacag
     6841 gagcgggcgt ggtcgctgca cggcatgccg aaccgcttct ggcgtgtgct cgcgatcgtc
     6901 gggctgttcg gtctcgtcaa cttccccgac acgctgctgc tgctgcgcgc caaggacctc
     6961 ggcttcgact tcgccggggt ggtggcgttg tacgtgctct acaacctgac ctacgccgcg
     7021 ggttcgtatc cggccgggag gttgtcggac cggtggtcgc gatcggtggt cgtggccgcc
     7081 ggactggccg tcttcgcggt ggcttacctg ggattcggac tgacctcgac ctcggcnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngact ggccgtcttc gcggtggctt
     7261 acctgggatt cggactgacc tcgacctcgg cgtgggcctg ggtcctgttc ccgctctacg
     7321 gctgctacac cgccctgacc gacggggtca tccgtgcgtg gatcgcagac ctggtacccg
     7381 cgcacagccg tggcaccgcc ctcggggtcc aggcggccgt ctcggggttc ggcctgttgg
     7441 tcgctggggt gtgggcgggt ctggcgtggg gcgggacggg ccgcgttccg ttcctcatct
     7501 cgggattgct cgtcgcgggc ctcgctctgc tcctgttcgc gctcggcccc tcgctcacgt
     7561 ctccaccgcc ctgaccggct ccagcggaat cgtccgtccc gttgccatac gcgggtcggc
     7621 ggtgcgatct ggatcgtcgg tgacaccggg tcgggcaggg tggaaccgcc cggcctgact
     7681 gccgtcatac cggtcgagtc cggtatcaga ctgataccgt ggtcgtgatg gccatgacgc
     7741 tacggctcga ccaagcggat caggatgcgc ttcgggaaca cgccgccgcc gaaggtatct
     7801 ccatgcagga cgttgcccgc cgagccatcc gggactacat cgccaggagc aaccaccgcg
     7861 ctcgcgtgtc gaacgcgacc gagctgatcc tgaacgtcca cgccgacgcg atcgagcgcc
     7921 tcggtcagtg agcgaaccgg tcgagttcct cgacctcgac gacctcgtcg acatggcacg
     7981 gatcctgctc ggcgaccctc cgccgatccg cgacatcgga ctcctcggat cggccgcggc
     8041 ccgtccccag accaccgtcg gcggccggga cgcctacccg accgtgtggt cgaaggcagc
     8101 cgcgctgctg cagtcggtgg tcgacaacca cgcgctcgtc gacgggaaca agcggctcgg
     8161 ctggctcgcc accgccgtct tcctcgagat caacgacgca agcgtggcag gcgcgaccga
     8221 cgacgaggtg ttcgacctcg tgtgggcgat cgcgtcagcc cgttcgacca tcgaggagat
     8281 cgctcaccac ctcgagatga tgagccggcg tcgatcgtga caccagactc accgccggga
     8341 ccgcaccaag aacagagcgt gggcgaagtc gtcgtcggca acgctcgcgc aatgcacaat
     8401 ctgtgggagg tcgggcgatc ggtggatctg cacgagctca cgctgattcc agcggtccgg
     8461 ccgtggaccg ccgggaccct tgaacgggag aggatgtctg acgtggccgg tcccgacccg
     8521 agtgctcacc acaggttcgt gcgcaggtcg agcgcctgtt cctccacggc cggcaccgca
     8581 ggagccccgt gctgatcatc accggaccgc cgggtgccgg gaagtcgacc gtcgccgcgt
     8641 cagtcgcaca gcactcggcc accgcagtgc gtgtcgaggc cgactggttc tggaccacca
     8701 tcgtcgccag gttcatcgac ccatggctgc ccgaggctga cgcgcagaac cgcacggtgc
     8761 ttcggtcggc gttccacgcg gcccgggcgt tcgaggacgg ggggtacgac accgtcgtcg
     8821 agggcatcgt cgggccgtgg atgctcgacg tcgtcgccga caccctgcgg gactcctcgc
     8881 ccgtggacta cgtcgtgctc cggccgtcac tcacgtcctg tctgagccgc gctgcccgac
     8941 gccccgccgt ggagcgggtt cccgggcacc ccccgctcga tcgatcagga ccgatccgcc
     9001 acatgtgggc ccggttctcc gatctcggcc gactcgagag ccacgccgtc gacacgtcga
     9061 ccatgtccga ggacgagacg gttcgagccg tgctggaggc acgtcacgac gggcgcctcc
     9121 gagtggagct cccgacgcca cggagttgac cggacgcgcc accgctcaag ccgtcgatcc
     9181 atgggaccca agcacgtccc ggagcgtcgg acttctcgtg tcgctcggcc gggccctgct
     9241 ggccttcccg agccgacaac cggcgaccgg gtcgtgcaac gatctgagga tgggaccttt
     9301 gaccgatcgc cgctaccttc gggacagcca ataccgcgag accgccaacc tggtcgcccg
     9361 atccaacctg cacgagcggt acgggcgcgg cgactggttc cgatggatcg cctcgcaggc
     9421 cccgtggccc gatcacggat gggtgctcga ggtcggctgc ggcaccggcc ggttgtgggc
     9481 cgacgctgcg gacatcgtgc cggacagctc cagagtcgtg ctgtgcgacc tgtcgccgac
     9541 gatggtccgc gagtccctcg ccgtcgccga tggcgcgacc ggtggatcgg tcgccgctct
     9601 gccccaactg ccgttccccg acgattcgtt cgatctcgtc atcgtgagca tggtgatgca
     9661 gcacctcgac gttccggagc cggcgatcgc cgagctcgcc cgggtcaccg ccccgcgagg
     9721 agcgctcgtc atcggcgcag ccagcgaaca gcacctcgac gagatcatgg tcctccgacg
     9781 tcgcttcggc gacgacgacg ccacgatcgg tggcgcgttc ggtgccggcg gactcgacgc
     9841 tgccaccgag cgcgtcgccc gctccttcag ccgagtcgac tggcagcgat acgaggacca
     9901 gctggtcgtc gacgaccggg aggcgatcgt cgactacctc cggtcctggt cccccctcga
     9961 cgacgccgac gatgacacgc tgcagcgact ccgcgccgag gtcgaccggc agttcgacgc
    10021 cgagttcgga gtgatgacca tcaccaagga cgtcgggtgc atcgtcgccc gttcaccgag
    10081 ttgacgcgag aacgcatcca cgacctgcgt ccgggctcga cggcgccggc tcgcatggca
    10141 gcctgaggac atggcctccc gtggggtgct ccgatgtctc gccgcagccg tgctcttcgg
    10201 tgcgtcggct cccgccgcgt cggtgctcgc cggcgacatg acggcgctcg tcctggccgg
    10261 gctcatgtac ctcggcgcgg cgctcgccgt cgcaccgctg tgcgtccgcc gacgaccccg
    10321 tcccgaggtg ctccgggcag ggtggcgtcc gctcggtctc gccgtcgtgt tcggcggcgc
    10381 gctcggcccg gcgttgctgg tcgcaggact cgcccaggtg cccgccgcca ccgcatcgtt
    10441 gatgctgaac ctcgagctgg tcgccaccgt cgctctggcg gccaccgtgt tccgcgagca
    10501 cctcggtcgc cggctgctcg cggcggcagc gcttctcacg atcgccggag tcctcctggt
    10561 gctcgagcca ggcgtcgggg tgcaaccggg cgcattgctg atcgtcggtg catgtgcgtg
    10621 ctggggcatc gacaacgcgg tgacggcccg gatcgatcag atctcgccgg agcaggtgac
    10681 cctggtgaag ggcatcgtcg ccgggtcctg caacgtcgtg atcggtctcg tcgtcgcggg
    10741 cggatgggga gtcagcacga gcgagttggc agccgcattg gccatcggcg ccgtcggcta
    10801 cggcgcatcc atcaccctct gggtcaaggg tgcccaagac ctcggcgcgg cccgcggaca
    10861 ggtcatcttc gcgactgccc cgttcatcgg cgcgctcatc tcctggaccg tgctgtccga
    10921 accggtgacc gcgacgcagg cgatcgctgt tccgatcgcg gccgtggggg tcgtcctgtc
    10981 gctgaggtcg agccacgagc acgagcaccg ccatcgcgtc acggtccacg agcacgagca
    11041 cacccacgac gacggccacc acgagcactc ccacgaggac ggcttcgccg gccgccacac
    11101 ccaccgtcac gagcacaggg atctggtcca cgcccaccct cacgtgcccg acctccacca
    11161 ccgccacgat cacggcatcg actgaacgct gacctgatcc gtaatgactc ctattcgacg
    11221 cacaatcgaa ggaattgagc gatagtgttg cttgtgtgag ttgggactcg acgtggatct
    11281 gatgttgttg agcgcggatg aggtggcgtc cgagctcggc gcccgggtgc gccgcgaacg
    11341 cctgcgccag aacatgactc agcgcacact ggccgaccgg gctggcgtca gtcgggcgac
    11401 actggctcgg atggagcagg ccggcacggc gacgcttccg aacttcctcg ccgtcctcgc
    11461 cgccctccgt cgcaccggcg acctgcagca gctcctggag ccgccgccca ccgagacgat
    11521 cggacacttc ctggcccgat cggagcccca gcgactgcgc ggccggcggt gagagccgtc
    11581 gatgtcgtct acgaaggtgc cgacggcccg tggatcgtcg cacgcgtcgc acagcgtggg
    11641 tccgagacct tcgtcgagta caccgacgag cacgtcgcga ccggagccga cctcgctccg
    11701 ctgaagcatc cggtggatca gcggagccgc agcttcccgg gcgcgacccc ggagcacctt
    11761 ccagggctgc tgtccgacgc gggtccggac tcctgggggc gcagcgtgct gcggcgtgac
    11821 atgcgtcggc acggtatcga cgctcccgaa cccctcgaca tgctcacttg gctcgggcgc
    11881 cggacgatgg gagcactgac cttccgcccg gtcgagggtc ccgagcccgc ggatgtcgca
    11941 ctcgtggagc tcgaccgctt gcaggacgaa gctcttcgcc acctcgctgg acaggacacg
    12001 cacgaccagg ccgccgaggc agttgcgctc gctgccggag gaagcgccgg cggggcccga
    12061 cccaagatca ccgcggctcg ctcacccgag ggagagctga tcgccgacaa cggagacctg
    12121 ccacccggct cgactccctg gctggtcaag ttccacggcc aggacgacag cgcatccctg
    12181 tccgtcgtgg aagcgtcgta cctggagatg gcacgaacag cgggcatctc cacctgtgag
    12241 cacgaactgc tccccggcag ctcgggaacg cagtacctgg cggtgcggcg cttcgacagg
    12301 accgtcgagc ccggagcgca gcccggccgg gtccacatgg ccacagcagc cggccttctc
    12361 gaggcgtacc ccgaactcgg ccagcacgtc ggctaccacg acgtcatccg cctgacgcgg
    12421 cgggtgaccg gagacgtgcg cgagaccacc gagatggtca ggcgggccgt gttcaacgtc
    12481 gtcgcccaca atcgcgacga ccacgctcgc aacatcgcct atctgtggac acccgccgac
    12541 ggatggcggc tcgcccccgc gttcgacctc acgttctcgc tcggcccgag accgacgttc
    12601 gcggccgccg gtcccggcga gcactacctc gacgtagcgg gcaaaggtgc ctccatcacc
    12661 cgcgacgacc tggtcgggct ccacaagccg gcgggcctcg agccccatgt ggtcgccgac
    12721 atggtcgacg aagccgtggc ggcagtcgac gagtggccga cggtcgcgga tcgcaacggc
    12781 gtcgacgcgg acaccatcga gcacgctgcg gcacggcttc cggcgctcgg tctctgacga
    12841 acggcgatcc gacggcgagg gacccgccac accgagcgcg accgatgcct cggatcgtca
    12901 gttgggcacg aggctgcccc gaggggtgac gaccggtcgt ggactgggag ctcgaagcag
    12961 accgggcgga gatcgctcgg ttgaccgaag caattgtgct cgcagtcatc tacatggtca
    13021 gccgctactg gatcgccacg ctcgtgtcgc ccgaggagac ctccgcccag gtccgcgtcg
    13081 tgttcgacca ggcgctcgtg gaccagggcg gactgctcac cgatgaccca gcccggctga
    13141 tcctgctagc tgtctctgac aacggcccgg ccaagactgt tgtgcgacac acatgctgac
    13201 aggtagggcg cacgcgactg gggagggtct ggggcatccg atcgggcggc agacgcgaac
    13261 gggcggggtc agctgcgagc gagccgatcg ctgacgagcc tgttcaagct gacgccttcc
    13321 tgtgcggcgg agagcgccag ttcacggtgc agggactcgg gaacgcgaac gttgaacttg
    13381 cccgagaagc gccgctcggt gatcggctcc ggaatcgggt caccgctcgc ctccatgtcg
    13441 gcgacgacgc cggcgacgag atcgaccagg ccctgtagcg ccttggtctg gtccgcgtgg
    13501 agccatgaca gtgacgggaa ctcggccacg gtggcgacga actcgtcgtc ctcggcagac
    13561 cacaggactc ggtaggcata atgcttgatg tcggtcactt ctgctcctcc tgcaccttgt
    13621 cgatggcctt gagtacctgt cgaacttggt aggacttcgc cttgccgtcc ttcgccttct
    13681 ggatgttcac ccgtggatca cccggccaag gggtcttgaa agcggcatgc gatgccccgc
    13741 tctggcgcgg ctccccgaag aagtggacgc acaccgagcg aagctcgttg taggagttgt
    13801 tctgcggtgc gttacgcatc gcggccacgg tcttgtcgat cttggccacc cgttgacggt
    13861 accagtatcg gtaccacctg acaaccgttc tggaccccgt tgcgcggccg ccccaccaac
    13921 gctccgcccc ccgttgtggc tgccctccac atgcctcccg cccgcggccc gccatcgcca
    13981 gccccagccg cacttctcca cggtgccccc ccatttccgc ctcccgagga gcgccagtcc
    14041 gagtagttcg cgaccgaacc gcgactagtc gcaccccaaa ggaaccgatt cggcaagctt
    14101 tcaggaccaa gaacccttgt agacactggg tgcccggggc gggattcgaa cccgcacgct
    14161 ccgaagagct gaggggtttg agccttcggc ccaccgtgac gactggtgtc gctcaggccg
    14221 agttggtggt gcccagtagg ccgttccggg cctccatcgc ggtgttcgtg acggctcgtg
    14281 cgttggagtg cgagagattc cgggtagttc gcgactactt ccgcgactgc tcacggccgg
    14341 tatcgaaccc tgcgagggcc ggcgtcagcc ggcgcggcgg accacggcgt ggccttcgcg
    14401 ctcgagcttc acgatcgtcc ggtgctccga gaggttctcc ccgactccga caacgagatc
    14461 cgctcccaca gcggccgcga ccttctccag tgtctcaagc gtcgggcgcg taccaccgcc
    14521 ttccatgcgg gcgatggccg actcggtggt gcccatgcgg tcggccaatt cggcctgggt
    14581 cagcccggcg tcactgcgga gtcggtggac gagctcccgg aaggccgtga ttcgcgcctc
    14641 atccgccgca gcgctgctcg tccacgagct gggcggtcaa cttggaactg agctcacgaa
    14701 tgcgaagtgc cgcggggaac tggtcgtggt tgaagacgac agatccgacg cccctgggag
    14761 gggggagatc gagaccggcg acctgttccc agtgctcgag cacggcgagc gagaactcgg
    14821 cgaaggtgga cctcacctcc ggcggaatgt ctggagacac ctgcaccctg gcgtgcagct
    14881 catcggccca ctcggcccat cgggcccgcg ggtactcaac tgcgaatccg gcagcggtct
    14941 tcgccgacca atcccggcag cgcatcgccc acagcgcgat gtccgtgtag agctgcgatg
    15001 cctgctccgc tcggcggtcc tgtcgcgctt ccgctgccgt gatcttcgcc gtcgatcttc
    15061 cgccgacgat ggccgcgccg gcgccgacga cggcgccagc gaagccagct ccggcgctga
    15121 caaggacttc tcccattccc accgtcctga cggtactgcc acgaagttcc gcgacgcggc
    15181 agagccatga gcacatcgcg gtccaggcct ccggcccaac gtgacaactg gtgccgctca
    15241 ggccgacctg gcatgcatcc ccatcacaga tggaagattc gttccatatt tgcgcgctag
    15301 ctgggtaacc tctattggat ggacttcgtc cgaccgatcg aggccatcgt gcccggcgct
    15361 caggggcggg tgctcgccgt gctcgccgag accacggccg agctgaacct gcggtcgatc
    15421 gcacagctcg ccgagatcag ccaggcccag acctcgcggc tgctcccgga gctcgttgat
    15481 ctcggcgtcg tggagcggcg agaagtgccg ccggcgtcgc tcttccggat cgtccccgaa
    15541 cacatcgctt cccgggcgct gctggcactc gcccgctccg ccgacgccgt cctggacgag
    15601 atggggcgac tggcccgtgc gcttccccac ccaccagcca gcgtgatcgt cttcggatcg
    15661 ttcgcccgac gcgaagccga tacccacagc gacatcgatg tcgtcatcgt tcgcccgaca
    15721 gagatcgacg aggacgacga cgaatggctc gactcgctcg acacgtggcg acgcgcggtt
    15781 cggcgactca gcggcaacgt cgtcgaggtg ctcgacgtca gcaccgacga ggccaccgcc
    15841 aagctcagag gccggagcca ggtgtggacc gacatccgcc gtggcggaca ggtcgtgcat
    15901 ggcctcagca tcgacgaact ccgggacgcc cgaagtggct aggccagcgc ggaccaggtc
    15961 ggtctcagcc gcgcaggtcc gctcatacgc ggcgaaggca cgggagttcg ccgaagccgc
    16021 agccagcgat ctccaggccg gtcgcaacat cgccgccaca agcctcgcca tccacgccgg
    16081 catcaacgcc gccgacgccg tctgcggcgc ccggctcgcc aagcgagcag caggcgagga
    16141 ccacgaccag gtactcgggc tgctcggcca agccggaccg gacggcgccg aagtcgctcg
    16201 cgacctccga cgactgcttc ccctgaagac caaggccgag tacgagcccg acgacatcgc
    16261 tccatccatc gcagcaaagg cggtcgaacg cgctcggcga tgcgccgcag tggcccagcg
    16321 agttgctgaa cggaggggct agccactcgg cctccgctgc gggcggcatc gacagaccga
    16381 gccgccgacg gccacggcgg aacgcccggt caggcgaccg cccgaagagg tacgcgaacg
    16441 gtgacttcgt gtaaccgacc cctcgaccgc tggcgtgcag tgacgccgtg gtgtcgtcgt
    16501 gcccggggcg ggattcgaac ccgcacgctc cggagagctg aggggtttaa gcgtcgatcc
    16561 accggtccgg gtggttcaag tgaagcccgt tccaccccgt cctgtcagcg atcgagcgat
    16621 cacgcggtcc gccagaaggc ggcccgtacc gcccagttcc ggcgcgttcc ctagcaatcc
    16681 gtgggcagaa accgagttca ccgaacgtga acaccaccac ggccgcaaca tgtgggtcac
    16741 ccgcaagggc gccatccggg cccgcaccgg tgatctgggc gtcatcccgg ggtcgatggg
    16801 cacccggtcg tacatcgtca tgggccgagg gtcatcggcg tcgtactgct cgtgttcgca
    16861 cggggcaggc cgccggctct cgcgcggccg agctcgccgc gagctggatc tggacggact
    16921 gcgggaacag atgccggcaa ggcgtggaac gacgctgatg ccgaagcgct catcgacgag
    16981 gacccgcggt cctacaagga catcgaccag gtcatggacg accaacagga cctcgtcgcc
    17041 gtccaccaca ccctgtccca gatcctcaac tacatgggcg tctgacgaca tccgcgacct
    17101 gctcggcgcg tcagccaccc aggacaggtc aacgagacct gaacgatgcg gcgagcgttg
    17161 gcttctcggc tctcagtcgc cgcattcggt gacgagtgcg tcggtgcggt cacccgccgg
    17221 gacagcggtg atgtcgaggt agtagccgcc ggcggtggag atcaggccgt tgacccacgg
    17281 ggtgccgccg ctgtcggttc cggtcttggt ctggatcggg tcatcgcccg tactcgacga
    17341 gtcgaaccag gccacggtcg cttcgggctt gccggggacg gacacgacgg cgacaagtcc
    17401 gctgttgcag tcgatcagtt gggagggggc gacgaggctg cggcctccgg cgggcacctt
    17461 gaacggtgag ccgaccaggt actcgtcgat cccgtcgtcg atggggtccc ccgggccggg
    17521 gagccgggtt gggtcgaggt tcgtggggag gttggcagcg accacagcgc cagtggggat
    17581 ggcggcagtt ggcggaacgt tgaccttgtc cgtgccggcg acggaggcga gtcctcgttc
    17641 gtagcggagc tgatccgttc cgcgccgtgg gaggtcggtg ctcgatgtcg tggttcccgg
    17701 actggaccgg atgaacaggt gggactcggg tcgaagtcgg caaccggacg cgggggtcag
    17761 cgagtcgcac tgcaccgcgg cccccaccgc catggacatg gccagcgggc cgtcggtgaa
    17821 atcgcagtcg agtcggttct caccgtccag gcgcggttcg gtgaggaacc ggctgggcgt
    17881 tccgtcgccc accgtgacca atgcggtggt cgaggtggcc gatgggtcac ccggcacggt
    17941 gaccgttcgg acgacacagc tttcgggtgc gtcaccgtcc ttgacacgca gcacctcggc
    18001 gtaccggttc cacacctcca ccggatcgcc gtcaacgacc aacagcgcct gccagccgac
    18061 gctggaacct gatccgtagc tgtccacagc cgggaacacc cgcccgacca ggcgggaccc
    18121 gtcctgaacc tgcaccccag atcccaatgg ggtgccaggg ggctgcagcc gtgagtcatc
    18181 cactccgacc aacgccgccg gacccgactt cgcccccgcg tccacggaac cggaaccggt
    18241 gtcgacccga tcaccacacc ccgacgcgac gagcacaacc gcagccgcgc tgagcaccaa
    18301 acggattgag acaagacggc tgcccgacga gcgcaacatc accccagcat catcccactc
    18361 cacccccgac cctgtgccgc gtcgacgcag gatcgatgca ggggcggttg cgaccttcag
    18421 agggctcgcc aaccaaggcg gtgcgcctcc gcctcagcga tctcaccgag gtagcgagcc
    18481 gagctcacgc cgagatcgac cgcggcgagc cggaggtcct tccacctcgc atcgtcgacc
    18541 tggatccgga ggaagcgctg gcgcggtgac ggttcacctt cccccggacc ccgtcgacgc
    18601 cgcgacgacg gtcgaccgga caagttgccg gcggcgagtt gatccatctc gaagcgcacc
    18661 agctccccca tcctccagat caggtacgtc ccttgcgcga cgacatcgcg ctgcagcacc
    18721 gcccacgcat cgtcatccac atcgaccctg actggacgac catctcgggg accgttccga
    18781 cgctgaccca gccgctcgaa ccgtcgccgc tcaaactcca accgatgctc cgccgcacga
    18841 gtctccttct ccgcctcggt acgcgcccgc tgcatgcggg aaatctctgc aagcagctca
    18901 cgcttacgcg tgagaagccg ctccgtgtgg gcgaccttct gctcctcggc gtcggcccgg
    18961 tccttgaggt cgaccaggtc ctgcgccttg tatcagcgcg catccgccgc ttctcggccc
    19021 ggtaccgggg gtggtcaggg ctgaccaagc ctgtgctcat ggggcggtca gccctgacca
    19081 acgcatgacc acgcgagtga catgccaaga atctccgtgt cccaccggcc gttcggtggc
    19141 acactggaca ggccgacggc acagagcccg aggtagtgaa ctcagttgat ggtcggacga
    19201 ccagcagctg ggtttgcgac tttttcagcc cacgtcgatg acgcgcaccg agttgccgag
    19261 ccggcgctgc cagtccccac ccgccccgac cagccaggcg tacgcatcgg cgagccacag
    19321 cagtggctcc ccggctccgc tcacgtgctc ataggtcagc ggtggcgtcg ggcgacgcgc
    19381 ggcccagatg atggggtggt cgtcggcatc ctggttgtgg cgggactcga gcgtcagtcg
    19441 gaccggctca ccggtcgact gcaggtcgag gacgatcgcc gccaggcagg ccgctcgcgc
    19501 ctgcgctgtc cgccggcctg gtgccgatcg acagacgaac acgctggcac cgacgtcgat
    19561 ggcgagcaga tcggcgacga gaaagcgttg tcgccgatcg ctctcgctgt ggaagtgcag
    19621 tcgacgctga ccggggagga gcaggccccg cgcagccgac ctggcccgac ccgcatccgc
    19681 tcggtcgatg acgacgcagc tcatgaggta gcggccgggc cgttcgctct cgtcgacgta
    19741 ggccagcagc tcggtcatgc cgaggagccg aagatgtcgg ccgccaccgt gtcggcggct
    19801 tcggcgtcca ggctgggcgc cacgtggctg taggtgtcga tggtgaccgc aatggtggcg
    19861 tggccgaggc ggtccgacac gaccttcggg tgcaccccgg cccggagcgc gagggtcgcc
    19921 caggtgtggc gcaggttgtg ggggccccgc agcggcggca ggtccgtcgc cttcacgtag
    19981 cgctggaact gccgggagaa ccggtccggg tgctccggcc gtcccgtgcc gtcacagagc
    20041 acgaggtcgt agctggggtc ccaggcctgg ccggccgtcg cccgctcctg gtcctgcacg
    20101 acccgatgag cgtggagcac gtcgacggtc gtggggtcga gcgagatcgt ccgacggctc
    20161 cggttggtct tggtcgggcc cagcaccagc ttgtcgttca ccgtcgtcac cgtctgggtg
    20221 accgaggccc gtcgggccga caggtcgacg tctcgccacc gtaggccgaa cacttcgccc
    20281 cgacgcatgc cagtggtggc cagcagcacg taggcagcga agagccggtc acccgaaacg
    20341 tgttccagga aggcggccaa ctcgtccgcg gtccacgttg ctcgctccac atgcggtacc
    20401 gcaggcggcc gggccaggtg cgcgacgttg cgcatcacca gccgcagtcg ctcggcgtcg
    20461 gccagcgccc ggtgcaacac gatgtgcgtg ttgcggaccg tcttggcggc caacgggcgg
    20521 ccggcgggac cgccctcgtt cacgaggcgg gcgtagaagc gctcgacctc cacggcggtc
    20581 agcgcctgga gcttgccggc acccaggccc cagcagatcc gctgcaccgc caccccgtag
    20641 ctggcccagg tcgtctcccg gagctgcggg cggaccgtgg tgagccactc ggtcaggtac
    20701 tcctcgaggg tgaccgacga gggcgccacc accgtgccgg agcggatctc gttgagcact
    20761 tcggtgaggg cgtcctcggc cgcggcccgg gtgcggaacc cgcccttggt gcgttgcttg
    20821 cgcttgccgg tggcgggatc ccgaccgacg tcgacgacgt agccccaggt gctccctcgc
    20881 ttgtggacat gcccgcgcat ggccgcaccg tagcccgaag tgcgttagca atctgtgagc
    20941 atgagccgat tctggacagg ggaccactcc ccggatcggc acgtttcgcc tgtcattgca
    21001 gggtgcccgg ggagagattc gaactctcac gctccgaaga gccgaggggt ttaagccctc
    21061 tgcgcctgcc agttacgcca cccgggcggg tgcccatg
//
LOCUS       DAOQOD010000035        20979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_866_length_20979_cov_10.833445, whole genome shotgun sequence.
ACCESSION   DAOQOD010000035 DAOQOD010000000
VERSION     DAOQOD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20979
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_866_length_20979_cov_10.833445"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3252)
                     /locus_tag="PLZ72_05445"
     CDS             complement(<1..3252)
                     /locus_tag="PLZ72_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA35775.1"
                     /translation="MGDVDHGADDDGADDDSADDADDADDGADVDGVAETDPDDAEDD
                     TDDAAEDAKWGLSDDAPIVGTGAPPVVAVMVTRDPGDWFEECLASIADQDYENLSLLV
                     VDNGSVDDPTARVADVLPSAFVRRLPEDRGFAAAANEALVSVEGAPFLLFVHDDVRLR
                     PDAVTAMVAEAFRANAGVVGAKLVDWDDEHVLRSVGLAVDVFGSSVPLVDPGELDQSQ
                     HDSARHVFAVSTACMLVRSDLFTTIDGFSAEIPFFGEDVDLCWRVHIAGASVQFCPRA
                     TVGHRERFSDRRPLEDRTRYETRHETRMVLANTELRRWWWTVPVGVVLGLVDLIGSAL
                     PGRLRHSGDVLAAGVWNLVHTPDLVRARARTRRARRVHDADYRTLFHKGSHRLRTLVR
                     TDDGEGRLAAATRSGRVALADMTSVSSRAAAGLVIATVIVALVGARHLISGPLPVMRE
                     LVSGGSSATDLVSQWWTAWRGVGLGESSVPVGLVPAFGTVGTVLLGSIGLARRLLILA
                     PLVIGAFGAWKMLSGTRSIRGRSAALAVYALNPIALNALATGRLQALVVYAAAPWLLR
                     RVARQAGAAPFADPDRPPVSWPRHLAGNGLLLGTVAAVSPLGSLLLAVTVSVFGVAAV
                     VGGERDRALRLPAAALGGLLFSLPLQLPWLVESITGGDAASLSGLWATRAALPSAAEM
                     MTGSIGPIQTGWLGWGLVVAALVPLLTGRAWRLGWTVGAWTVILGSLALSVLLASSSL
                     LGGAGVALFLMPTALGLSVAVAMAPLAFEDDVVSGDFGVGQLASAVGVVAMVIGLVPV
                     LFAAPEGRWYLPEGDYQRALELVDRSDAGRTLWIGDPDVLPESGWRLNGPDDLAVGVT
                     EGHLPTVTQRYRLDGGSGVEHLRAAVRAALDGRTSRVGQLLAPMGIRYVLLIDRPAPE
                     PFSSLEVPLPHGAVAAFQEQLDLTRIPVAPGARLFEVAGAWPLRSDITELDLPANGVP
                     TLAGQILRPMPRPPAVLGRGPGTSFSGELPDGADVAQAVTADEGWTLVTGSGTADRSG
                     LFGWAQRFRVDEGGESTLSWNTPVAARILQLIQVVGLVVLIAMVGRRRRLAAPR"
     gene            3658..4566
                     /locus_tag="PLZ72_05450"
     CDS             3658..4566
                     /locus_tag="PLZ72_05450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA35776.1"
                     /translation="MKALITGAGGFVGHHLIDHLNACGDEVMTTDRSIDGLDITDAPA
                     LLESFRRQRPEVVYHLAGASDVGGSWSTPQATFRSNAEGTLNVLWAAREAGVDRVLTV
                     GSADVYGKVAVEDLPIRESLAMRPVSPYAASKASADHVALQAHLGFGQSVVRARPFNH
                     LGPGQSNRFVAAALAERVARNELDDESHVRVGNLSPRRDFTDVRDVVRAYRLLVEHGV
                     DGEVYNVCSGQAIAVQALADELIALARIPMELVIDPDLERPVDIPVLLGDNSRLCADT
                     GWTPEIPLTTTLADLLDDQRRRVHTT"
     gene            complement(4656..5171)
                     /locus_tag="PLZ72_05455"
     CDS             complement(4656..5171)
                     /locus_tag="PLZ72_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35777.1"
                     /translation="MAAFDDDPPVTTSHPPTSSHLGSSGPERLGERIARLRVAAGMTQ
                     AELAHRVAISRVALSNLESGRNVPGERTITLLAGVFGLEPPELVAGTAYPSSKAERLP
                     LVTARHTEVQLRLALLERDLRWLEGAPVAVAARVAAEWRRELVELADRASGPAEIAAV
                     DAARRRMAERT"
     gene            5238..6218
                     /gene="gmd"
                     /locus_tag="PLZ72_05460"
     CDS             5238..6218
                     /gene="gmd"
                     /locus_tag="PLZ72_05460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA35778.1"
                     /translation="MTRRALITGITGQDGSYLAELLLDEGYEVIGMVRRSSTVTFERI
                     AHLQDRIATVPGDLLDQASLIDLLRTYRPTEVYNLAAQSFVQTSFSQPVLTGDVTGLG
                     VTRLLDAIRIVDPDIRFYQASSSEMFGKVVEVPQTESTPFYPRSPYGVAKQYGHWITV
                     NYRESYDLHASSGILFNHESPRRGLEFVTRKITNTVAKIKLGLTDELRLGNLDAERDW
                     GFAGDYVRAMWLMLQQDSPDDYVVATGETHSVRRFCEIAFGHVGLDHEQFVVIDEAFY
                     RPAEVDLLIGSPAKAESTLSWTPTVGFEDLVTMMVDADIDLLSGRLGPIG"
     gene            6309..7310
                     /gene="cofD"
                     /locus_tag="PLZ72_05465"
     CDS             6309..7310
                     /gene="cofD"
                     /locus_tag="PLZ72_05465"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNA35779.1"
                     /translation="MTTETAAPMPTGRVAVLCGGVGAARFLRGLLEVHDPTAVTGIVN
                     VGDDSIIHGLHISPDLDTITYTLAGADNPDTGWGLRGETWQAMEQLRAHAAANAIDAA
                     ADAAGWFSLGDRDLGTHLYRTSRLAQGAALSQITAEICRTFDLRLRLVPVTGDPVRTR
                     LRAADGRDLSFQEYFVRERHSVEVASVRFDGADRARPDPAAIDALESASAVIIAPSNP
                     IVSIDPVLAVTGVRDLVSSRRDRTVAISPIVGGAALKGPADRLMRELGLEPSVVGVAR
                     HYAPIASTLVIDPADEALADRVEAEGIRAVVTPTVMSAPGVASALATTCVGLAEGRA"
     gene            7307..8035
                     /gene="cofE"
                     /locus_tag="PLZ72_05470"
     CDS             7307..8035
                     /gene="cofE"
                     /locus_tag="PLZ72_05470"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNA35780.1"
                     /translation="MTGGIELIPVRGLGEVTRGTDLAAMICDAVELVHHDVVVVTQKV
                     VSKAEGAMERVDPDDPLSHKPLVERESVRILRRRGDLIISETRHGFVCANAGIDLSNV
                     ERGWAALLPEDPDRSARRIRDGIRGRTGTEVGVVVSDTFGRTWRRGVTDVAIGSAGLI
                     PVLDLRGTADAYGRELQVTEVAVVDEIAGAADLVMGKSTGVPVAIVRGLGDEVFSPRR
                     DDGTEEHRGVLGDVVRSPAEDLFR"
     gene            complement(8072..8821)
                     /locus_tag="PLZ72_05475"
     CDS             complement(8072..8821)
                     /locus_tag="PLZ72_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35781.1"
                     /translation="MERFADKVVLITGAASGIGRATAERLASEGAALALSDVNAEGLE
                     ATSETCRAAGARVFAQVSNVADEAQVADLVAAAVSEHGRLDVAVNVAGILHFEDLRRT
                     ELADWDRIIAVNLTGTFLVCRAVMDHLLPTGGNIVNTSSTSALAGHPWTVSYSASKGG
                     VYALTQCLAVEFGKQGVRCNSVAPGSILTPIQNEFRLPEGADVKLINRITALDEHRGP
                     ETVASAIAFLASDDAAHVNGTCLKVDGGTLA"
     gene            complement(8930..9877)
                     /locus_tag="PLZ72_05480"
     CDS             complement(8930..9877)
                     /locus_tag="PLZ72_05480"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNA35782.1"
                     /translation="MRALVTGGAGFIGSNLVDRLLAEGHAVDVVDNLSSGKLTNLADA
                     RADRDHEFTFHQMDICDPAVSELIERRSPEVVFHLGAQIDVRVSVADPALDARINVLG
                     TINVLEGARRAGARKVVFASSGGTIYGDVDPDDLPVGEGHSQHPVAPYGVSKKVATDY
                     LHAYRELHQLEYTSLALANVYGPRQDPHGEAGVVAIFAGKLLAGEPCTVFGDGAQTRD
                     YVFVDDVVDAFVRAAERGSGLLCNIGTGVESSVLDLYATMAANAGVTEPATHAPARMG
                     ELARSCLDASRAELHLGWRPFTDLSAGTAVTLDWFRERG"
     gene            complement(9969..10985)
                     /locus_tag="PLZ72_05485"
     CDS             complement(9969..10985)
                     /locus_tag="PLZ72_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNA35783.1"
                     /translation="MKAVILVGGFGTRLRPLTLSVPKQMLPMGGVTMLQRVVEHLGGL
                     GVEEVVLSLGYQPEVFLKEFPDGEIAGVPVRYAVEPEPLDTGGAIAFAARHAGIDSTF
                     LALNGDVLTDLDVAQLWAFHRSTGGAATIALTEVEDPSRYGVVPLAADGRVEAFIEKP
                     EPGTAPTHWVNAGTYVLEPDVLDLVPDGARCSIEREVFPALVAQRRLFGLQASAYWID
                     AGTPEAYLQAHLDMLDGLRGSFEPAVSDSARVDAGAMLKRSMVGAGATVAVGVRVVDS
                     VVMAGARISAGASITRSIIGARAVIGEGCELSELSVVGFDEDVPAGTVEAGGHFPPPT
                     EWPA"
     gene            complement(10987..11757)
                     /locus_tag="PLZ72_05490"
     CDS             complement(10987..11757)
                     /locus_tag="PLZ72_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35784.1"
                     /translation="MGKASSSKKIRRVQQAGVSRAPGQRRNLAYPALIVGIIVVGTLL
                     VFLARDSRQATASEAPTTRDHWHEALGINICGEFLGNPSDDGPDRTGIHTHADGLIHI
                     HPFGSGATGDNATMQVFLDQIGASVTDDSLTLKDGTTKTNGDKCDGKAGEVALFVWPP
                     QASDKSEPEKFTGTDITEHRFTEDGGAYVLSFNPKGYTPKLPPSMTELANPSDVEPGQ
                     STGGLNSATSSTVPAAPSTPAESTTTAAPAESTTSAPR"
     gene            complement(11835..13088)
                     /locus_tag="PLZ72_05495"
     CDS             complement(11835..13088)
                     /locus_tag="PLZ72_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA35785.1"
                     /translation="MNILVLCPHYAPDVAPTGEVMTAIVEALAARGHRLHVVTALPWY
                     RHHAIEDGWAGRPARTESTPWGWITRLHPFPTDKTNIPARAVAFAGFTGMATVRAAFT
                     RIRPDVVMVMSPPLPLGVAGWLAAIPRRTPFVFNIQDVFPDVAVELGAITDPRVIAAA
                     SWLERFLYRRADAVTVLSDDLRDNVAGKLGRHRPERVVVIPNFVDTERIRPTDPDNSY
                     RAENDLTGRTVVMYAGNVGLSQSLDLVVEAARRFRDRGRDDVVFVINGGGSALAGLRE
                     SARGLDNLRFVGMQPRERLPEVLAAGDLHLVPLRTGLARSSVPSKLYSILAAGRPVLA
                     SVDGGTEVATTIERAGAGLSVPPEDADRFVAALERMLSDRTALAAMGASGRAFVEGWV
                     SPAAVGAAYEQLFESVRVGRFRTGA"
     gene            complement(13108..14262)
                     /locus_tag="PLZ72_05500"
     CDS             complement(13108..14262)
                     /locus_tag="PLZ72_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNA35786.1"
                     /translation="MTRVLVNLLWLVPGVVGGSEESTTDALRAVADVLDRDGSGGDLD
                     IHLAVLEQFAHAHPDLAERYDLVVADQDGRDKRRRVWAEQTWLAARTRALRPQVVHHA
                     GGVVPFVHPGRTVLTIHDLQPLDLPHNFRPAKRNYIRLMAARSARAADIVTVPSRFTR
                     ERVVERLRVAPARVEVVPWWVSPPRHQDTSEPFPLPPMLGGDRFVLYPAITYPHKRHD
                     VLLDAFALVAARHPRLRLVLTGGAAASESAVQTRIRRPDLDGRVLRTGRVPAAQLESL
                     FAAAALVAVPSDYEGFGLPVLEAMVRGVPVVAAAAGSLPEVARPEDLVPAEDVAAWAA
                     AIEAVLAESTDARNARIAGGRERAAQFGPERTAAALLTAYRRAAAARVPT"
     gene            complement(14259..15437)
                     /locus_tag="PLZ72_05505"
     CDS             complement(14259..15437)
                     /locus_tag="PLZ72_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNA35787.1"
                     /translation="MPPDDPTLPRPDRDVAARRVALTLTQLSHRVPGGTATSVLDLAR
                     ALIERRDVEVVVVGARGLTRRPPVLGSDVEVTVVDLPLPARILYDAWDRTGRPAVDSA
                     VADGAVADRAGAADLVHLTVPMGPPRSAVPLVATVHDLFPLSRPEWFTRRGVRLMAPA
                     LMRIRDRADAVVVPSRSVADDCLAHGFDPDRLHVVPWGSRPVRLPADGAESVRRRHGL
                     VGPYVLFVGTVEPRKGLPVLTDALARLGRPELTLVVAGPAGWGPALDGELGTVPGPVV
                     RTGYLSDADLAALRAGASVCCLPSFAEGFGLPALEALAAGTPVVTTARTALAEVVGDA
                     AVLVPAGRPDRLADALRRVLDDEDLAAALRAAGPERAATFSWERTAESTAEIYRAVTP
                     "
     gene            15521..16498
                     /locus_tag="PLZ72_05510"
     CDS             15521..16498
                     /locus_tag="PLZ72_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNA35788.1"
                     /translation="MVGDPHSDFWRHRKVLVTGGNGFLGSAVLRELHDVGAAEVVAPS
                     AAEYDLRRRDDAEAMIGLHSPDLVIHLAAKVGGIGANMERPSDLYLDNLLMGTHLLDA
                     TLRARTPKTVVIGTICSYPKFTPVPFSEDSLWQGYPEETNAPYGIAKLAQLVQLQANR
                     DQYGQSAIYLMPTNLYGPGDKFHPSVSHVIPALIKKCVDAAESGADSIEVWGTGTASR
                     EFLYVDDAAHGIVLAAQLYDGRDPVNLGTDEEMPIRDLVGLICELVGFDGEIRWDTTK
                     PDGQPRRRVDPSRARDAFGFEARVPFREGLRRTIDWYRENREQAEARDS"
     gene            complement(16536..18023)
                     /locus_tag="PLZ72_05515"
     CDS             complement(16536..18023)
                     /locus_tag="PLZ72_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNA35789.1"
                     /translation="MGRATARGSGRTGRTWPQRILIGANVVLVLACLGIAAGLTKIRT
                     TLEQVPVVDVGGALSAPVDVGQPRNVLIIGTDSHKGLDKSDPVVKGRLEGENLADVIM
                     VLRVDPTDGSARLLSIPRDSKVELPDGSKQRINAAIGGPQGPKTLVQTIKRNFGISID
                     NYVEVDFAAFKDLVEVLGTVPVYFTTPVRDRTTGLYIDNAGCIPLNPDQALAYARSRH
                     FEFKQNGKWKTDGTGDLGRISRQQDFMKRAMRRASDQGIRNPSTATGMVDAAASAVVL
                     DDTLNVGTILDLLGQFRSFNPDDLVTYQVPTEAAPSGGVAYQAVIWDQAMPMLRPFWS
                     QGDTRQPADVIVDVKGSSKASDTLQSLSDQLDAAGFDAEPVEARGAGSATTITHGPLG
                     RDAAVLLARYLGTVPKLVEDPDIIGFRVVLNVGSPAPTVRSEPLSIDQLPPALTTTTT
                     TPPPPGDGTVPEESSTTTTVLEGAPPDAAPPGIVPTDPEKAAACH"
     gene            18119..19150
                     /locus_tag="PLZ72_05520"
     CDS             18119..19150
                     /locus_tag="PLZ72_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNA35790.1"
                     /translation="MVTADVALAVVGGRLCTDLRDVTADLSALDSTGFWAVVIPFEGP
                     PVCARFAEVRPATRWPGAPWVGPDPTAWSTSLDRDRFCSGVDSIRELIRAGDVYQVNL
                     TRRLSAPLPAAPDGTTTDIAALGAALASGNPAPFSAVVRLPDHGIHVASASPERFLSR
                     DGDVLRSSPIKGTAVDEESLLDKDRAENVMIVDLVRNDLGRVCEWGSVTVPSLLAVEQ
                     HPGLVHLVSTVEGRMRPGAGWADALDATFPPGSVTGAPKEAALAVIGALEPVPRGVYC
                     GAVGWVDADRRRGDLNVAIRTFWIEDGWLHLGTGGGITIDSDPEGEWQETELKARNLL
                     RVARGMVRS"
     gene            19147..19995
                     /locus_tag="PLZ72_05525"
     CDS             19147..19995
                     /locus_tag="PLZ72_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNA35791.1"
                     /translation="MTDPVVLWLDGELVDPAAAAVAWNDHGLTVGDGVFETIELRGGA
                     AFALTRHLDRLERSCHGLGLDPPDRTRLSGAVDAVSSAWGTSGGRLRITVTSGAGPMG
                     SERGDAAPTLIVSATSMTVQRDPTAVVVVPFTRNENGALAGLKTTSYAENVVALRLAR
                     AVGAGEAVLANTAGELCEGTGSNVFVGHDGVLVTPPLSSGCLAGVTRALLLEAMAAAG
                     QPAVEAALPVGALAGATEAFLVSTGRHVQPISHVDGVALPAAPGPLTERAARIWARAY
                     ADEVDP"
     gene            complement(19996..20808)
                     /locus_tag="PLZ72_05530"
     CDS             complement(19996..20808)
                     /locus_tag="PLZ72_05530"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNA35792.1"
                     /translation="MQFERAKAALAGTRFADFRTVEETGSTNADMVEILRTQDPAEDR
                     RPAVLVADHQSAGRGRRDRTWDAPPGSSLLMSIGLPVGDIDRSRWPLVNAAVALAAVD
                     AAPDLRIKWPNDLVEVGSGPGGADLKVGGILAELHEDLGGMGACMIVGIGLNLNWGQM
                     PPELADTATSLDIILGGDVVADVIVTDLLVSLDSKWLGMLGRSAASIEPFVDAYRSRS
                     TTIGRHVRVELDGDRELVGTAVDVDDSGALVVEGEDGTRSTVTVGDVVHLRV"
     gene            20825..>20979
                     /locus_tag="PLZ72_05535"
     CDS             20825..>20979
                     /locus_tag="PLZ72_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNA35793.1"
                     /translation="MFSKVLIANRGEIAVRVIRACREMGITSVAVYSDLDRDALHVRL
                     ADEAYALG"
BASE COUNT         3017 a         7493 c         7657 g         2812 t
ORIGIN      
        1 ccgtggggcg gcgagccgtc ggcggcgacc caccatcgcg atcagcacca cgagcccaac
       61 cacctggatc agctgcagaa tccgggcggc gaccggggtg ttccacgaca acgtggactc
      121 cccaccctcg tcgacccgga accgctgggc ccacccgaac aacccgctgc gatccgccgt
      181 gcccgacccg gtgaccagcg tccatccctc gtcggccgtg accgcctgcg cgacgtcggc
      241 accatcgggc agctcgccgg agaagctggt cccggggcct cggccgagca ccgccggggg
      301 acgcggcatc ggtcgcagga tctgaccagc cagcgtcggc accccattgg cgggcaggtc
      361 gagctcggtg atgtcggagc gcagcggcca ggccccggcg acctcgaaca gccgggctcc
      421 cggggcgacc gggatccggg tcaggtccag ctgctcctgg aacgccgcga ccgccccgtg
      481 gggcaggggg acctcgagcg acgagaacgg ctccggggca ggtctgtcga tcagcagcac
      541 gtagcggatg cccatcggcg cgagcagctg tccgacccgg gaggtccgtc cgtcgagggc
      601 ggcccggacg gcggcgcgca agtgctccac ccccgaccct ccgtcgagcc ggtagcgctg
      661 ggtgaccgtg gggaggtggc cctcggtcac ccctaccgcc aggtcgtcgg gaccgttcag
      721 gcgccacccc gactccggca ggacgtcggg gtcgccgatc cacagggtgc gaccggcgtc
      781 gctgcggtcg accagctcca gggcgcgttg gtagtcgccc tcgggcaggt accagcggcc
      841 ctcgggtgcg gcgaacagca ccggaaccag accgatcacc atggcgacga cgcccaccgc
      901 cgaggcgagc tgcccgacac cgaagtcgcc gctgaccacg tcgtcctcga acgcgagcgg
      961 cgccatcgcc accgcgaccg acaggccgag cgcggtcggc atgaggaaca gcgccacgcc
     1021 cgcgccgccg agaagcgacg acgacgccag cagcacgctc agcgcgaggg aaccgaggat
     1081 gacggtccac gcaccgacgg tccagcccag gcgccaggcc cgcccggtca gcaacggcac
     1141 cagcgccgcc acgaccaggc cccaccccag ccagccggtc tggatcggcc cgatgctgcc
     1201 ggtcatcatc tccgccgccg atggcagcgc cgcccgggtc gcccagagtc cgctcagcga
     1261 cgccgcgtcg cccccggtga tcgactcgac cagccagggc agctgcaacg ggaggctgaa
     1321 caggaggccg ccgagggcgg ccgcgggcaa ccgcaacgcc cggtcacgtt cgccgccgac
     1381 caccgcggcc accccgaaca ccgacaccgt caccgccaac agaagcgacc cgagcgggct
     1441 caccgcggcg accgtgccga ggaggagacc gttgccggcc aggtgtcggg gccacgagac
     1501 cggggggcgg tccggatccg cgaagggcgc ggccccggcc tgacgggcga cccgccgcag
     1561 cagccacggg gccgccgcat agacgacgag ggcctggagc cgtccggtcg ccagcgcgtt
     1621 cagggcgatc gggttgagcg cgtagaccgc cagggccgcg gaccgcccgc ggatggaccg
     1681 cgtgcccgac agcatcttcc aggccccgaa cgcgccgatc accagcggcg ccaggatcag
     1741 caaccgacgg gcgagcccga tcgacccgag caggacggtg cccaccgtgc cgaacgcagg
     1801 aaccagaccg accggaaccg agctctcccc gagtcccacg ccgcgccacg ccgtccacca
     1861 ctgcgacacg agatcggtcg cgctcgaccc gccgctgacc agctccctca tgaccggcag
     1921 aggtccgctg atcaggtgtc gagctcccac cagggcgacg atcaccgtgg cgatcaccag
     1981 gccggctgcc gcccgactgc tcaccgaggt catgtcggcg agcgcgacgc gtcccgaacg
     2041 ggtggcggcg gccagacggc cctcgccgtc gtcggtgcgc accagcgtcc gcagccggtg
     2101 gctccccttg tggaacaggg tccggtagtc ggcgtcgtgg acccgtcggg cccgtcgcgt
     2161 ccgggcgcgg gcccgcacca ggtccggggt gtggaccagg ttccacaccc cggcagcgag
     2221 gacgtcaccg ctgtggcgga gccgcccggg gagagccgag ccgatcaggt cgaccaggcc
     2281 caggacgacg cccaccggga cggtccacca ccagcgccgc aactcggtgt tggccaggac
     2341 catgcgggtc tcgtggcggg tctcgtagcg ggtgcggtcc tcgagcgggc gtcgatccga
     2401 gaagcgctcg cggtggccaa cggtcgcccg ggggcagaac tgcaccgatg ccccggcgat
     2461 gtggacccgc cagcacaggt cgacgtcctc tccgaagaag gggatctcgg cgctgaaccc
     2521 gtcgatcgtg gtgaacaggt ccgagcgcac gagcatgcat gcggtcgaca ccgcgaagac
     2581 gtgacgggcg ctgtcgtgct gggactggtc gagctcaccc gggtcgacca acggcaccga
     2641 gctgccgaag acgtcgacgg cgaggccgac cgatcgcagc acgtgctcat cgtcccagtc
     2701 gacgagcttg gcaccgacca cgccggcgtt ggcccggaac gcctcggcga ccatcgcggt
     2761 caccgcgtcg ggacgcaggc ggacgtcgtc gtgcacgaac agcaggaacg gggctccctc
     2821 gaccgacacc agggcctcgt tggccgcggc cgcgaacccc cggtcctcgg ggagtcgacg
     2881 gacgaaggcg gacggcagca cgtcggccac ccgggcggtg gggtcgtcga cggacccgtt
     2941 gtcgacgacg agcagggaga ggttctcgta gtcctggtcc gcgatggacg cgaggcactc
     3001 ctcgaaccag tcccccggat cgcgggtgac catgaccgcg acgaccggcg gcgctccggt
     3061 gcccacgatc ggggcgtcgt cggacagccc ccacttggcg tcctcggccg cgtcgtcggt
     3121 gtcatcctcg gcatcgtcgg gatcggtctc ggccaccccg tcgacatcgg cgccatcgtc
     3181 ggcatcgtcg gcatcgtcag cgctgtcatc gtcagcgccg tcatcgtcag cgccgtggtc
     3241 gacgtcgccc acgcctggag gcccctcgtc ctcgacggag cccggcccgg tctcgatcag
     3301 ccgagcatcg ctcggcaccg cagccgtggg ggacacgtcc gcgctcggct ccgcgaggtc
     3361 atcgggctcg ctctcgcggg gatctccgtc ggcgtcaccg gctcccggag gtggagtgga
     3421 ggggatcgtc ctggcctttc tgaggcaggg gcccgcatcg acgctcaccg cgcgaggtgg
     3481 acccagcgga gggggtggga cggatggcga tcgtagtgcg gtccccctcc ccgaccggtt
     3541 catgccggcg acgcccgagg ggacggcggt ccgtcccggt ctccgatcgg caccttctag
     3601 agtcatcgcc atggccacac ccggctccaa ggcacgccga ccgacccgga acctccggtg
     3661 aaggcgctca tcaccggagc cggcggcttc gtcggccacc acctcatcga ccacctgaac
     3721 gcctgcggcg atgaggtcat gaccaccgac cgctcgatcg acggtctcga catcaccgat
     3781 gcccccgccc tgctcgagtc gttccgacgt cagcgcccgg aggtggtgta ccacctggcg
     3841 ggagcgagcg acgtcggcgg ttcgtggtcc actcctcagg cgacgttccg ctccaacgcc
     3901 gagggcacct tgaacgtgct gtgggccgct cgggaggccg gggtcgaccg ggtgctgacc
     3961 gtcggttccg cggacgtcta cggcaaggtc gccgtcgagg acctgccgat cagggagtcg
     4021 ctggcgatgc gtccggtgag tccgtacgcg gcgtccaagg cctcggcgga ccacgtggcc
     4081 ctccaggcgc acctgggctt cggccagtcg gtcgtgcgcg cccgaccgtt caaccacctg
     4141 ggcccgggcc agagcaaccg cttcgtcgca gcggcgctgg ccgagcgcgt cgcccgcaac
     4201 gagctcgacg acgagagcca cgtgagggtc ggcaacctga gccctcggcg ggacttcacc
     4261 gacgtccgcg acgtggtgcg ggcctaccgg ttgctcgtcg agcacggcgt cgacggcgag
     4321 gtctacaacg tgtgcagcgg ccaggcgatc gccgtgcagg cgctggccga cgagctcatc
     4381 gccctggccc ggatccccat ggagctggtg atcgatcccg acctggagcg cccggtcgac
     4441 atcccggttc tgctcggcga caactcccgc ctgtgcgccg atacgggctg gacgccggag
     4501 atcccgctga cgaccacgct ggccgacctg ctcgacgacc aacgccgcag ggtgcacacc
     4561 acctgacgcg cactccgtcg cgccgccaac tcgctttgtc ccgcacagac gtcgtgatca
     4621 cgacgtctgt gcgggacaaa gcccgggggg agggctcagg tccgctcggc catgcgtcgg
     4681 cgtgccgcgt cgaccgccgc gatctcggcg ggacccgagg cccgatccgc caactcgacc
     4741 agctcacgtc gccactcggc ggcgacccgc gccgccaccg cgaccggagc gccctcgagc
     4801 catcgcagat cccgctccag cagcgccagc cggagctgga cctcggtgtg gcgggcggtg
     4861 acgagcggca gccgctccgc cttggacgac gggtacgcgg tgccggcgac gagctcgggc
     4921 ggttcgagcc cgaaaacccc ggccagcagg gtgatcgtgc gctcccccgg gacgttgcga
     4981 ccggactcca ggttcgacag cgcgacccgc gagatcgcca cccggtgggc gagctcggcc
     5041 tgggtcatgc ccgcggccac gcggagccgg gcgatccgct ctcccaggcg ctcgggcccg
     5101 gacgagccga ggtgggagga ggtcgggggg tgcgacgtcg tcacgggagg gtcgtcatcg
     5161 aaggccgcca tcggcgggag cagatcctgc caactgggtt ggcagacggg agacggccac
     5221 gagcgtagaa cagagccatg acccgacgtg cgctcatcac cggcatcacc ggccaggacg
     5281 gctcctacct ggccgagctc ctcctcgacg agggctacga ggtgatcggc atggtccgac
     5341 ggtcctcgac ggtcaccttc gagcgcatcg cccacctcca ggaccgcatc gcgacggtcc
     5401 ccggcgacct gctggaccag gccagtctga tcgacctact gcgcacctac cgtccgaccg
     5461 aggtgtacaa cctggcggca cagagcttcg tccagaccag cttcagccag ccggtgctca
     5521 ccggggacgt caccgggctc ggtgtcacac gactgctcga cgccatccgc atcgtcgacc
     5581 ccgacatccg cttctaccag gcgtcgagct ccgagatgtt cggcaaggtg gtcgaggttc
     5641 cccagaccga gtcgacgccg ttctaccccc gctcccccta cggcgtggcc aagcagtacg
     5701 gccactggat cacggtcaac taccgcgaga gctacgacct gcacgcctcg agcgggatcc
     5761 tgttcaacca cgagtcaccg cgccgcggcc tggagttcgt gacccgcaag atcaccaaca
     5821 cggtcgccaa gatcaagctc ggcctcaccg acgagctccg gctcggcaac ctggacgccg
     5881 agcgcgactg gggcttcgcc ggcgactacg tgcgggcgat gtggctgatg ctgcagcagg
     5941 acagccccga cgactacgtg gtcgccaccg gcgagaccca ctccgtgcgg cgcttctgcg
     6001 agatcgcctt cggccacgtc ggcctggacc acgagcagtt cgtggtgatc gacgaggcgt
     6061 tctaccggcc cgccgaggtg gacctgctga tcggatcacc ggccaaggcc gagtccacgc
     6121 tgagctggac gccgacggtc ggtttcgagg acctggtcac gatgatggtc gacgccgaca
     6181 tcgacctgct gtcgggccgc ctcggcccga tcggctgacc ccgcccccgc tttgtcatgc
     6241 gtggacgtcg tgatcacgac gtccacgcat gacaaagcgg tgaggtggct gacggtaggg
     6301 tccggccgat gacgaccgag accgccgccc cgatgccgac ggggcgagtc gccgtgctct
     6361 gcggcggggt gggcgccgcc cggttcctga gggggctgct cgaggtccac gacccgacgg
     6421 cggtcaccgg catcgtcaac gtcggcgacg actcgatcat ccacggactg cacatcagcc
     6481 ccgacctgga cacgatcacc tacaccctcg ccggcgccga caaccccgac accggatggg
     6541 gtctccgcgg cgagacgtgg caggcgatgg agcagctccg ggcccatgct gcggccaacg
     6601 cgatcgacgc cgctgccgac gccgccggct ggttctcgct cggcgaccgc gacctgggaa
     6661 cccacctcta ccggacgtca cggctggccc agggtgccgc cctgagccag atcaccgccg
     6721 agatctgccg caccttcgac ctgcgccttc gcctcgtccc cgtcaccggc gacccggtgc
     6781 ggacccgcct gcgagccgcc gacgggaggg acctgtcgtt ccaggagtac ttcgtgcgtg
     6841 agcgccactc ggtcgaggtg gcgtcggtcc gcttcgacgg agccgaccgt gctcggccgg
     6901 atcccgcggc catcgacgcg ctcgagtctg cctccgcggt catcatcgcc ccgtcgaacc
     6961 cgatcgtgtc gatcgacccc gtcctggcgg tcaccggggt ccgcgacctg gtgagctccc
     7021 gacgcgaccg caccgtcgcc atcagcccca tcgtcggcgg agccgccctc aagggtcccg
     7081 ccgaccggct gatgcgcgag ctcggcctcg aaccctcggt ggtcggagtc gcgcgccact
     7141 acgccccgat cgcgtccacc ctggtcatcg atccggccga cgaggccctg gcggaccggg
     7201 tcgaggccga ggggatccgg gccgtcgtga cccccacggt catgagcgct ccaggggtgg
     7261 cctcggccct cgccacgacc tgtgtgggcc tcgcggaggg gcgggcgtga ccgggggcat
     7321 cgagctgatc cccgtccgcg gactggggga ggtcacccgc ggcaccgacc tggccgccat
     7381 gatctgcgac gcggtcgagc tcgtccacca cgacgtggtg gtcgtgaccc agaaggtggt
     7441 gtccaaggcc gaaggggcga tggagcgcgt cgatccggac gacccgttgt cgcacaagcc
     7501 gctggtcgaa cgcgagtcgg tgaggatcct ccggcggcgc ggggacctga tcatctccga
     7561 gacccgccac ggcttcgtgt gcgccaacgc cggcatcgac ctgtccaacg tcgagcgggg
     7621 ctgggcggct ctgctgcccg aggatccgga tcgctcggcc cgacgcatcc gcgacgggat
     7681 ccggggtcgg accggcaccg aggtcggcgt ggtcgtgtcg gacacgttcg ggcgcacctg
     7741 gcggcggggc gtgaccgacg tggccatcgg atcggcaggg ctgatcccgg tcctggacct
     7801 gcgggggacc gccgatgcct acggtcgtga gctgcaggtg accgaggtgg ccgtggtcga
     7861 cgagatcgcc ggcgcggccg acctggtgat gggcaagtcg accggcgtgc cggtcgcgat
     7921 cgtgcgggga ctgggcgacg aggtgttcag cccccgccgc gacgatggca ccgaggagca
     7981 ccggggggtc ctcggcgacg tcgtccgctc ccccgccgag gacctgttcc gctgacgggg
     8041 cccgaacccg tcgggcggcc cgggtcgctc gtcaggccag ggtgccgccg tcgaccttga
     8101 ggcaggtgcc gttgacgtgc gcggcgtcgt cggaggcgag gaacgcgatg gcgctggcga
     8161 ccgtctcggg cccgcggtgc tcgtcgaggg cggtgatccg gttgatcagc ttcacgtccg
     8221 ccccctcggg cagacggaac tcgttctgga tcggggtcag gatcgatccg ggagcgacgc
     8281 tgttgcagcg cacgccctgc ttgccgaact cgaccgccag gcactgggtc agcgcgtaga
     8341 ccccgccctt ggacgccgag tacgacacgg tccacgggtg gccggccagc gccgaggtcg
     8401 acgaggtgtt gacgatgttt ccgccggtgg gcaggaggtg gtccatcacc gcccggcaca
     8461 ccaggaacgt cccggtcagg ttcaccgcga tgatccggtc ccagtcggcc agctcggtcc
     8521 gccgaaggtc ctcgaagtgc aggatgcctg ccacgttgac cgccacgtcg agccgtccgt
     8581 gctcggacac cgcggcagcc accaggtcgg cgacctgggc ctcgtcggcg acgttcgaca
     8641 cctgggcgaa cacccgcgcg cccgcggcgc ggcacgtctc ggaggtggcc tcgagacctt
     8701 cggcgttgac gtcggagagg gccagcgcgg cgccctcgga tgccagtcgt tccgccgtgg
     8761 cgcggcctat gcccgacgcg gccccggtga tcagaaccac cttgtcggcg aagcgctcca
     8821 cctggtcacc ccctggcgga cccggccgcc gtccccggtc gactgctggt cgaccgaccc
     8881 gccgagctga cgggacgtca gatcatagga gccgacgagg ccctcgacct cagccccgct
     8941 cccggaacca gtccagggtg acggcggtgc cggcgctcag gtcggtgaag ggtcgccacc
     9001 ccaggtggag ctcggcccgc gacgcgtcga ggcaggaccg ggccagctcc cccatgcgcg
     9061 ccggggcgtg ggtggcgggt tcggtcaccc ccgcgttggc cgccatggtc gcgtacaggt
     9121 cgagcaccga ggactcgacc ccggtgccga tgttgcacag cagaccgctg ccccgctccg
     9181 cggcccggac gaaggcatcg accacgtcgt cgacgaacac gtagtcgcgg gtctgcgcgc
     9241 cgtcaccgaa gacggtgcac ggctctccgg ccagcagctt cccggcgaag atggcgacca
     9301 cacccgcctc gccgtgcggg tcctggcggg ggccgtagac gttggccagc gccagggagg
     9361 tgtactcgag ctggtgcagc tcccggtagg cgtgcaggta gtcggtggcg accttcttgg
     9421 acacgccgta cggagcgacg gggtgctgtg agtggccctc gccgacgggc aggtcgtcgg
     9481 ggtcgacgtc gccgtagatg gttccacccg acgaggcgaa gacgaccttg cgggcaccgg
     9541 cccgacgggc gccctccagc acgttgatgg tgccgagcac gttgatgcgg gcgtcgaggg
     9601 cggggtcggc caccgagacc cggacgtcga tctgggcgcc gaggtggaac acgacctcgg
     9661 gggaccgccg ttcgatcagc tccgagaccg ccgggtcgca gatgtccatc tggtggaagg
     9721 tgaactcgtg gtcgcggtcg gctcgggcgt cggccaggtt cgtgagcttg ccgctcgaca
     9781 ggttgtcgac gacgtccacg gcgtgaccct cggccaggag ccgatctacc aggttcgagc
     9841 cgatgaaccc ggcccctccc gtcaccagtg ctctcatgtg gtccccctca ctctcgtcgc
     9901 ccctccgctg gacggtccga tccgaccggg gctgctcgta cggtgtgcca accggtccgg
     9961 cgacgtgctc acgccggcca ctcggtgggg ggcgggaagt ggcccccggc ttcgacggtg
    10021 ccggccggga cgtcctcgtc gaagccgacg accgacaact ccgacagctc gcacccctcg
    10081 ccgatcacgg cgcgggcccc gatgatggat cgggtgatgc tcgccccggc cgagatccgg
    10141 gcgccggcca tgaccaccga gtccaccacg cgtaccccga cggcgacggt ggcgccggcg
    10201 ccgaccatcg accgcttgag catcgctccg gcatcgacgc gggcggagtc cgacaccgcg
    10261 ggttcgaacg agccgcgcag gccgtcgagc atgtccaggt gggcctggag gtaggcctcg
    10321 ggcgtcccgg cgtcgatcca gtaggccgac gcctggagcc cgaagagacg cctctgggcc
    10381 accagggcgg ggaagacctc tcgctcgatc gagcagcggg cgccgtccgg caccaggtcg
    10441 agcacgtcgg gctcgagcac gtaggtgccg gcgttgaccc agtgggtggg ggccgtgccg
    10501 ggctcaggct tctcgatgaa ggcctcgacg cgcccgtcgg ccgcgagcgg gacgacgccg
    10561 taccgggacg ggtcctccac ctcggtcagg gcgatggtgg cggcgccgcc ggtggagcgg
    10621 tggaacgccc acagctgggc gacgtcgagg tcggtgagga cgtcgccgtt gagggccagg
    10681 aacgtgctgt cgatcccggc gtggcgggcg gcgaaggcga tcgcccctcc ggtgtcgagc
    10741 ggctcgggtt cgaccgcgta gcgcacgggc accccggcga tctccccgtc ggggaactcc
    10801 ttgaggaaga cctcgggctg gtagccgagc gagagcacga cctcttcgac acccagcccg
    10861 ccgaggtgct cgaccacgcg ctggagcatg gtgacgccgc ccatcggcag catctgcttg
    10921 ggcaccgaca gcgtcagcgg gcggagtcgg gtgccgaagc ccccgacgag gatgacggcc
    10981 ttcatgtcag cgaggggcgg acgtcgtcga ctcagcggga gcggccgtgg tcgtcgactc
    11041 ggccggagtg ctcggcgccg ccggcacggt gctcgacgtc gcggagttga gaccaccggt
    11101 ggactgaccc ggctcgacgt cggaggggtt ggccagctcg gtcatcgagg gcggcagctt
    11161 gggggtgtag cccttcgggt tgaacgacag cacgtaggcg ccgccgtcct cggtgaagcg
    11221 gtgctcggtg atgtcggtcc cggtgaactt ctcgggctcg gacttgtcgc tcgcctgcgg
    11281 cggccacacg aacagcgcca cctcgccggc cttgccgtcg cacttgtcac cgttggtctt
    11341 ggtggtcccg tccttgagcg tcagcgagtc gtcggtgacg ctggcgccga tctggtcgag
    11401 gaacacctgc atcgtggcgt tgtcgccggt ggcgccggac ccgaaggggt ggatgtggat
    11461 cagcccgtcg gcgtgggtgt ggatgccggt gcggtccgga ccgtcgtcgg acgggttgcc
    11521 caggaactcg ccgcagatgt tgatcccgag cgcctcgtgc cagtggtccc gggtggtcgg
    11581 cgcctcgctg gcggtggcct ggcggctgtc gcgggccagg aacaccagca gcgtccccac
    11641 gacgatgatc ccgacgatca gggccggata ggccaggttc cgacgctgcc cgggggcacg
    11701 gctgacgccg gcctgctgga cgcgccggat cttcttggag gaggaggcct tgcccatacg
    11761 ggcctagacg gtagcgccgc gacggccctc gggccgaacc cgccccaggg gctgctcagg
    11821 acccccgtcc ggggtcaggc cccggttcgg aatcgaccga cccggaccga ttcgaacagc
    11881 tgctcgtagg cggcgccgac cgcggcgggt gatacccagc cttcgacgaa cgcccggccc
    11941 gatgcaccca tggcggccag ggcggtgcga tccgacagca tccgctcgag ggcggcgacg
    12001 aaccgatcgg cgtcctccgg ggggaccgac aggccagcgc cggcccgctc gatcgtggtg
    12061 gcgacctcgg tcccgccgtc gacgctggcc agcaccggcc gtcccgcggc caggatcgag
    12121 tacagcttgg acggcacgct cgaccgggcc aggccggtgc gcagcggcac caggtgcagg
    12181 tcgcccgcgg cgagcacctc ggggaggcgt tcccgcggct gcatccccac gaagcggagg
    12241 ttgtcgagcc ctcgggccga ttcccgcagg cccgccaggg cggagccccc tccgttgatg
    12301 acgaacacga cgtcgtcgcg cccgcggtcg cggaagcgtc gggccgcctc gacgacgagg
    12361 tccagcgact gcgacaagcc gacgttgccg gcgtacatga ccaccgttcg ccccgtcagg
    12421 tcgttctcgg cgcggtagga gttgtcgggg tcggtggggc ggatgcgctc ggtgtcgacg
    12481 aagttcggga tgacgaccac ccgttcgggc cggtgccgac cgagcttgcc ggcgacgttg
    12541 tcacgcaggt cgtcgctgag cacggtgacc gcgtcggcac gccggtacag gaagcgctcg
    12601 agccacgacg cggcggcgat gacccggggg tcggtgatgg ctccgagctc gacggccacg
    12661 tcggggaaca cgtcctggat gttgaacacg aacggcgtgc gccgggggat cgccgccaac
    12721 cagccggcga cgcccagcgg gagcggcggc gacatgacca tgacgacgtc gggacggatc
    12781 cgggtgaaag cggcgcgcac cgtcgccatg ccggtgaagc cggcgaaggc gacggcgcgg
    12841 gcggggatgt tggtcttgtc tgtcgggaac gggtggagcc gggtgatcca accccacggg
    12901 gtcgactcgg tgcgcgccgg tcggccggcc cagccgtcct cgatggcgtg gtggcggtac
    12961 cacggcagcg ccgtcaccac gtgcagacgg tgcccccgcg ctgcgagggc ctcgacgatg
    13021 gcggtcatca cctcgccggt gggggcgacg tcgggggcgt agtgggggca gaggacgagg
    13081 atgttcatga gcggggtgca gcggcttcta cgtcgggact cgggcagcgg cggcacgacg
    13141 gtaggccgtg agcagggcgg ccgccgtgcg ctccgggccg aactgcgccg cccgctcccg
    13201 acccccggcg atgcgagcgt tccgggcgtc tgtcgactcg gcgaggacgg cctcgatggc
    13261 agcagcccag gctgccacat cctcggcggg caccagatcc tcgggtcggg cgacctcggg
    13321 cagcgacccg gccgccgcgg cgaccaccgg cacgccgcgc accatcgcct cgagcacggg
    13381 caggccgaaa ccctcgtagt cggaggggac cgccaccagt gccgccgcgg cgaagagcga
    13441 ttcgagctgc gccgcgggaa cccgaccggt gcgcaggacc cggccgtcga ggtccggccg
    13501 ccggatccgg gtctgcaccg cgctctccga cgcggccgca cccccggtga ggaccagacg
    13561 cagtcggggg tgtcgggccg caaccagggc gaacgcgtcg agcaggacgt cgtggcgctt
    13621 gtgggggtag gtgatcgccg ggtagaggac gaatcggtcg ccgcccagca tcggcggcag
    13681 cgggaacggc tcggaggtgt cctggtgtcg gggcggcgag acccaccacg ggacgacctc
    13741 gacccgggcg ggggccaccc gcagccgttc gacgacccgc tcccgggtga accggctcgg
    13801 cacggtgacg atgtccgcgg cgcgcgccga acgggcggcc atgagccgga tgtagttccg
    13861 cttggccgga cggaagttgt ggggcaggtc gagcggctgc aggtcgtgga tcgtcaggac
    13921 cgtccgcccg gggtgcacga acgggacgac tccgccggcg tggtggacca cctgcgggcg
    13981 cagcgctcgg gtgcgggccg ccaaccacgt ctgctccgcc cacacccgcc ggcgcttgtc
    14041 gcgaccgtcc tggtccgcca cgaccaggtc gtagcgctcg gccaggtcgg ggtgtgcgtg
    14101 cgcgaactgc tcgaggaccg ccaggtggat gtcgaggtcg cctcccgatc cgtcccggtc
    14161 caagacgtcg gcgaccgccc tgagcgcatc ggtcgtcgac tcctcgctgc cgcccaccac
    14221 gcccggcacc agccacagca ggttcaccag cacccgggtc acggcgtcac cgcccggtag
    14281 atctcggcgg tcgactccgc ggtgcgctcc cagctgaacg tggcggcccg ctcgggcccg
    14341 gcggcgcgaa gggccgcggc caggtcctcg tcgtcgagca cccgacgcag ggcgtcggcc
    14401 aggcggtccg gacgacccgc ggggaccagc acggcggcgt cgccgaccac ctcggccaac
    14461 gcggtgcggg cggtggtcac caccggggtt ccggctgcca gcgcctcgag ggccggcaag
    14521 ccgaacccct cggcgaacga cgggaggcag cacaccgatg cgccggcgcg caacgcggcc
    14581 aggtcggcgt cgctcaggta tccggtgcgg acgaccgggc cgggcaccgt tcccagctcc
    14641 ccgtcgaggg caggacccca gccggcgggg cccgcgacca cgagggtcag ctccggccga
    14701 ccgagtcggg cgagggcatc ggtcagcacc ggcaggccct tgcggggctc gaccgttccg
    14761 acgaacagca cgtacgggcc gacgagcccg tgccgtcgac ggaccgactc ggcgccgtcg
    14821 gcggggagcc gcacgggtcg ggaaccccac ggcacgacgt gcaggcggtc gggatcgaag
    14881 ccgtgtgcga ggcagtcgtc ggccacggag cgggacggga ccacgaccgc gtcggcacgg
    14941 tcccggatcc gcatgagcgc aggcgccatc agtcggaccc cccgtcgggt gaaccactcg
    15001 ggacggctga gcgggaacag gtcgtgcacg gtcgcgacca gggggacggc gctgcggggc
    15061 ggtcccatcg gaaccgtcag gtggacgagg tcggctgcac ccgcccggtc ggccaccgca
    15121 ccgtcggcca cagcgctgtc gaccgccggg cgaccggtcc ggtcccacgc gtcgtagagg
    15181 atccgtgccg ggagggggag atcgaccacg gtcacctcca cgtcggagcc gaggacgggc
    15241 ggccgtcggg tcagcccacg ggctcccacg acgacgacct cgacgtctcg ccgctcgatg
    15301 agcgcgcggg cgagatcgag cacggaggtg gccgtgcccc ccgggacccg gtgcgacagc
    15361 tgcgtgaggg tgagggcgac gcggcgcgca gcgacgtcac gatcggggcg cggcagggtc
    15421 gggtcgtcgg ggggcaccgt cggccagtct ctcgcacctc cggcacgccc ggaagcatgc
    15481 acgccacgag cgcccacccg agatggcgta gctttcggcg atggtcggag atccgcacag
    15541 cgacttctgg cggcaccgga aggtgctggt gacgggcgga aacggcttcc tgggctcggc
    15601 ggtgctccgg gagctccacg acgtcggcgc cgccgaggtg gtcgcgccga gcgccgccga
    15661 gtacgacctg cgtcgccgcg acgacgctga ggccatgatc ggcctccact cccccgacct
    15721 ggtcatccac ctggccgcca aggtcggcgg gatcggcgcc aacatggaac ggccgtcgga
    15781 cctgtacctc gacaacctcc tgatgggcac ccacctgctc gacgccaccc tgcgggcacg
    15841 gacccccaag acggtggtca tcggcacgat ctgctcctac ccgaagttca cgccggtgcc
    15901 cttcagcgag gactcgctgt ggcagggcta ccccgaggag accaacgctc cgtacgggat
    15961 cgccaagctg gcccagctgg tgcagctcca ggccaaccgg gaccagtacg gccagtcggc
    16021 gatctacctg atgccgacca acctctacgg gcccggcgac aagttccacc cgtcggtcag
    16081 ccacgtgatc cccgcactca tcaagaagtg cgtcgacgcc gccgagtccg gtgccgacag
    16141 catcgaggtc tgggggacgg gcaccgcgtc acgggagttc ctctacgtcg acgacgccgc
    16201 gcacgggatc gtgctggcgg ctcagctcta cgacggacgc gacccggtca acctgggcac
    16261 cgacgaggag atgccgatcc gcgacctggt gggcctgatc tgcgagctgg tcggcttcga
    16321 cggcgagatc cgctgggaca cgaccaagcc ggacggtcag ccccgtcgtc gggtcgaccc
    16381 cagccgggcc cgcgacgcgt tcggcttcga ggcccgggtg ccgttccgcg agggcttgcg
    16441 ccggacgatc gactggtacc gcgagaaccg ggagcaggcc gaggcccgcg acagctgacg
    16501 accggcggag ccgacgaccg cccgggcccg gtgggtcagt ggcaggcggc ggccttctcg
    16561 gggtcggtcg gcacgatccc gggcggagcg gcgtccggcg gggcgccttc caggaccgtc
    16621 gtcgtcgtgc tcgactcctc cgggacggtg ccgtcgccgg ggggcggcgg cgtggtggtc
    16681 gtggtggtca gcgccggcgg gagctgatcg atcgacagcg gttcggagcg gacggtcggc
    16741 gcggggctgc ccacgttgag cacgacccgg aacccgatga tgtcggggtc ctcgaccagc
    16801 ttgggcaccg tccccaggta gcgggccagc agcaccgccg cgtcccggcc cagcggaccg
    16861 tgggtgatcg tggtcgcgct cccggcgccg cgggcctcga ccggctccgc gtcgaagccc
    16921 gccgcgtcga gctggtccga cagcgactgg agggtgtcgg aggccttgga cgaacccttg
    16981 acgtcgacga tgacgtccgc gggttgacgg gtgtcgccct gggaccagaa cggcctcagc
    17041 atcggcatcg cctggtccca gatgaccgcc tggtaggcca ctcccccgct cggcgccgcc
    17101 tcggtaggca cctggtaggt gaccaggtcg tcggggttga agctgcggaa ctgcccgagc
    17161 aggtccagga tggtcccgac gttgagggtg tcgtcgagca cgaccgccga cgcggcggcg
    17221 tcgaccatgc cggtggcggt gctcgggttg cggatgccct ggtccgacgc ccggcgcatg
    17281 gcccgcttca tgaagtcctg ctgacggctg atccggccca ggtcgccggt gccgtcggtc
    17341 ttccacttcc cgttctgctt gaactcgaag tggcggctgc gggcgtaggc gagcgcctgg
    17401 tccgggttca gagggatgca gcccgcgttg tcgatgtaga gcccggtcgt gcggtcccgg
    17461 acgggggtgg tgaagtacac cggaacggtc cccagcacct cgaccaggtc cttgaacgcg
    17521 gcgaagtcga cctcgacgta gttgtcgatc gagatgccga agttgcgctt gatggtctgc
    17581 acgagggtct tgggaccctg cggtccgccg atggcggcat tgatgcgctg cttggagccg
    17641 tcgggcagct ccaccttgga gtcgcgcggg atcgacagga gccgggccga tccgtcggtc
    17701 gggtcgaccc gcagcaccat gatcacgtcg gccaggttct cgccctcgag ccgacccttg
    17761 accaccgggt ccgacttgtc gaggcccttg tgggagtcgg tgccgatgat cagcacgttg
    17821 cggggttgcc cgacgtcgac cggggccgac agcgcgccac cgacgtcgac gaccggcacc
    17881 tgctccaggg tggtgcggat cttggtgagg ccggccgcga tccccaggca cgccagcacc
    17941 agcacgacgt tggccccgat gaggatgcgc tggggccaag tgcggccggt gcggcccgac
    18001 ccgcgtgctg tcgctcgtcc cacgatcggc cgacggtagc agcggccgcg gtcccctccg
    18061 tggcatgccc ccgtccactc gggggccctc tcaggatccc cggccgtagg atcggccgat
    18121 ggtcaccgcg gacgtcgccc tcgccgtcgt cggcggccga ctgtgcaccg acctgcgcga
    18181 cgtgaccgcg gatctgagcg ccctcgactc gaccgggttc tgggcggtcg tcatcccgtt
    18241 cgagggaccg ccggtgtgcg cccgcttcgc cgaggtgcgt cccgccacgc gctggccggg
    18301 cgccccgtgg gtcggtccgg accccaccgc gtggtcgacc agcctggacc gcgaccggtt
    18361 ctgctccggg gtggactcga tccgggagct gatccgcgcc ggcgacgtct accaggtcaa
    18421 cctgacccga cgcctcagcg cgccgctccc ggccgccccc gacggcacca ccaccgacat
    18481 cgctgcgctg ggagccgcgt tggcttccgg gaacccggcg ccgttctcgg ccgtcgtgcg
    18541 tctgccggac cacggcatcc acgtggccag cgcgtcgccg gagcgcttcc tgtcccgcga
    18601 cggcgacgtg ctgcgctcct cgccgatcaa gggcacggcg gtcgacgagg agtcgctgct
    18661 cgacaaggac cgggccgaga acgtcatgat cgtcgacctg gtgcgcaacg acctgggacg
    18721 cgtgtgcgag tggggttcgg tcacggtccc gtcgctgttg gcggtggagc agcaccccgg
    18781 cctggtgcac ctggtcagca ccgtcgaggg ccggatgcgt ccgggggcgg gatgggccga
    18841 cgccctggac gccacgttcc cgcccgggtc ggtgaccggc gcacccaagg aggcggcgct
    18901 ggcggtcatc ggagcgctcg agccggtgcc gcggggcgtc tactgcggcg cggtcggctg
    18961 ggtcgacgcg gatcgacgac ggggcgacct gaacgtggcg atccggacgt tctggatcga
    19021 ggacgggtgg ctgcacctgg gcaccggcgg cggcatcacc atcgactcgg accccgaggg
    19081 cgagtggcag gagaccgagc tcaaggcgcg caacctgctg cgggtcgcgc gggggatggt
    19141 gaggtcgtga ccgatccggt ggtgctgtgg ctggacggcg agctggtcga tcccgccgcc
    19201 gcggcggtgg catggaacga ccacggcctg accgtgggcg acggcgtgtt cgagacgatc
    19261 gagctgcgcg gcggcgccgc gttcgcgctg acccgccacc tggaccggct cgagcgcagc
    19321 tgtcacggac tggggctgga cccacccgac cgaacccggc tgagcggtgc ggtcgacgcc
    19381 gtgagctcgg cgtggggaac ctcgggcggg cggctgcgga tcacggtgac cagcggggcg
    19441 ggaccgatgg gatccgagcg aggtgacgcc gcacccaccc tgatcgtctc cgcgacgtcg
    19501 atgacggttc agcgcgaccc gaccgcggtg gtggtggttc cgttcacccg caacgagaac
    19561 ggcgccctgg ccggtctcaa gacgacctcg tacgccgaga acgtcgtggc gctgcgcctc
    19621 gcccgcgccg tcggcgccgg tgaggccgtc ctggccaaca ccgccggtga gctctgcgag
    19681 ggcaccggct cgaacgtgtt cgtcggccac gacggcgtcc tggtgacacc gcccctgtcg
    19741 agcggttgcc tggccggggt gacccgcgcc ctgctgctcg aggcgatggc cgcggcgggg
    19801 cagcccgcgg tcgaggccgc tctaccggtg ggggcgctcg ccggggccac cgaggcgttc
    19861 ctggtgtcga ccggtcgtca cgtgcagccg atcagccacg tcgacggggt ggccctgccc
    19921 gcagcaccgg gcccgctcac cgaacgggcc gcccggatct gggcccgggc gtacgccgac
    19981 gaggtcgacc cgtgatcaga cccgcaggtg cacgacgtcg ccgacggtga cggtgctgcg
    20041 ggtgccgtcc tcgccctcga cgacgagcgc tcccgagtcg tcgacgtcga cggcggtgcc
    20101 gaccagctcg cggtcgccgt cgagctcgac gcggacgtgg cgcccgatcg tggtcgaccg
    20161 ggaccggtag gcgtcgacga acggctcgat gctcgccgcg gaacggccga gcatcccgag
    20221 ccacttgctg tcgagcgaga cgagcaggtc ggtgacgatc acgtcggcga cgacgtcgcc
    20281 gcccaggatg atgtcgagcg acgtggcggt gtcggccagc tcgggtggca tctggcccca
    20341 gttcaggttg aggccgatgc cgacgatcat gcaggcgccc ataccgccga ggtcctcgtg
    20401 gagctcggcc aggatccctc cgaccttcag gtcggcaccg ccggggccgg agccgacctc
    20461 caccaggtcg ttgggccact tgatccgcag gtcgggcgcc gcgtcgacag cggccagggc
    20521 caccgctgcg ttgaccagcg gccagcggga ccggtcgatg tcgccgacgg gcagaccgat
    20581 cgacatcaac agcgacgagc ccggcggcgc gtcccatgtc cggtcccgtc gtccccgacc
    20641 ggcgctctgg tggtcggcga cgaggaccgc gggacgacgg tcctcggcgg ggtcctgcgt
    20701 gcgcaggatc tcgaccatgt cggcgttggt cgagccggtc tcctcgacgg tgcggaagtc
    20761 cgcgaagcgg gtgcccgcga gagctgcttt ggcgcgttcg aactgcatca ggagaagata
    20821 ggtcgtgttt tccaaggtgc tgatcgcaaa ccggggtgag atcgcggtcc gggtcatccg
    20881 ggcgtgccgc gagatgggga tcacctccgt ggccgtgtac tccgacctcg accgggacgc
    20941 gcttcacgtg cgcctggccg acgaggccta cgccctggg
//
LOCUS       DAOQOD010000036        20968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_868_length_20968_cov_10.836465, whole genome shotgun sequence.
ACCESSION   DAOQOD010000036 DAOQOD010000000
VERSION     DAOQOD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20968
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_20968_cov_10.836465"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..3787
                     /gene="dnaE"
                     /locus_tag="PLZ72_05540"
     CDS             233..3787
                     /gene="dnaE"
                     /locus_tag="PLZ72_05540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNA35794.1"
                     /translation="MPDSFVHLHQHTEYSMLDGAARIGDVVASAVADGQPAMGITDHG
                     NMYGVLDFYKACRDSGVTPIIGTEAYMAHDSRFERPPRRGKVDDSGGDVEGGRKLYYH
                     LTLLAENDVGYRNIIQLASRAYMEGYYYKPRVDWDTLAEHHEGVIATTGCLGGHVLQS
                     LMNEGFDAALAKAARLQDIFGRDNLFVEIQDHGIPEQHRTNPQLLEIARKLQAPLLAT
                     NDSHYVHRDDAVSHDALLCVQTGSLMSDPDRFKFHSDEHYLKTAAEMRHLFAEAPESC
                     DNTLWIAERCNVEIEFGKPQLPNFPLPEGFTDDAEYLEHLTFEGARKRWGDQLPDNVV
                     ERLAYELKVIGDMGFSSYFLIVWDLIRHARDANIRVGPGRGSAAGCAVAYTLWITDLD
                     PIKYDLLFERFLNPSRISMPDIDMDFDSRYRDEMIRYAAERYGRDHVAQIVTFSTIKA
                     RAAVRDAARVLGKPYIVGDKVAKAMPPLVMGRDTPLYACLEEHPKFVDGYKMAAELRE
                     MYATDPETREVVDVAKGLEGLRRQDGIHAAAVVITKEPLTEYLPIQRKPESGVPIEEA
                     PVVTQYEMHGVEELGLLKMDFLGLRNLDVISDTLELIRRTRGVEVDIDDVDLTDEPTY
                     ELLRRADTIGVFQLEGGAMRALIRSLAPTEFEDVAALVALYRPGPMAANMHTDYADRK
                     NGRQEVTYLHPDAEEILGDTYGLCIYQEGIMRIAQRFAGYSLADADNLRKACGKKVRE
                     LIAKERTKFVEGCEATGYGAELGTAWFDIIEPFADYAFNKSHSYGYGFVAYQTAYLKA
                     NYPAEYLSALLTSVKTNLDKAAVYLAECRAMGIEVTVPDVNRSLSDFAPVVPEPDGDG
                     ANLDGGADRILFGLSAVRNVGTGLVELIIEERDENGPFEDFYDFCQRVNTQVLNKRTI
                     ESLIKAGGFDSMGHPRQGLLTVFEQIVDTTIARRKEHDMGVMSLFGEMEEGPAFDERV
                     RIPDVEFDKKHRLAFEKEMLGLYVSDHPLLGAEQALKRKVDCTILELGDLDDGTQRTV
                     GGVITGLQRKWTKKGDLMAVFVLEDLQGSVEVMVFPKTMAQIGHLLVEDAVVITTGRV
                     DKRDETAKFIPRELELFEPQVDEHPPLRLHLPPSRLDNATIDRLQELIGDFPGDSEVH
                     ILLGDKQVLRLSDDHLVDTRTGLIGELRALLGHESVVL"
     gene            3976..4647
                     /locus_tag="PLZ72_05545"
     CDS             3976..4647
                     /locus_tag="PLZ72_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35795.1"
                     /translation="MALDVTTKDCTALSDAELAEMADLCADGGICYEIGLLSKLVSDW
                     VLVTQVREGAHLRAFSFSTLERIGGTPSVLIGVAGIRRTSKRDSFLRAIVSDNLRRAL
                     MAFPDEDVLVGTRMVDPGAFEAYKVMVDIVPRPGHKVSGEERAWGRRLAKRFEVDAER
                     YDQHTFTVTGDGAAPLVLDHEPLKPEKVDPDVAAQFTHLETDRGDCLITFGWVMAEEL
                     LKYNG"
     gene            4644..5330
                     /locus_tag="PLZ72_05550"
     CDS             4644..5330
                     /locus_tag="PLZ72_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNA35796.1"
                     /translation="MTRVDPDPASPDPADPGGRAAFSQVLASRRMCRDFKPDPVADDV
                     LDRVLAAAFRAPAAGNTDALDLVVLAGADTARHWDVTLPADRRPGFRWPGLMRAPVLV
                     EVIVDPAAYVERYSLPDKAHSGLGDGAEAWPVPFWFVDGGAAVMAVLLAAESEGLGAL
                     LFGVFGHEEAVRRALGVPAGRRVVATVALGLPAPGGRLPSRSARRGRPGPDRRVHRGG
                     WAGQGPAPSG"
     gene            5400..8111
                     /locus_tag="PLZ72_05555"
     CDS             5400..8111
                     /locus_tag="PLZ72_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA35797.1"
                     /translation="MMQRFWSWLAVNLGKHAVIVTAVLAAFTILVGTGITRLEFATGQ
                     DSYLNAKDQVYKDNVVYQKLFGGEAMLSVITMDEGHTVDELFTPEGLATFQSVTEEIE
                     GIEGVQGVISPVTALQFSSDLVTPPEGGTPFDSIAAKALLHASEVAEKSGDTASAQAR
                     SADTAATAGRLLAVPADQQVLSNPAWVKFLLLDNNDQVRKALLPFFPDKTHALMITRL
                     DGNMSIEQEGTTSDAVVAATKDLQFPNSSTVTTGAPILLKNINDYLKGGMLTLGGIAV
                     LAMILILLLFFSVRWRLLPLAVVLVGVVWAFGFAGYLGIPLTLVTIAGLPVMLGIGID
                     YAIQMHSRIEEEVIIAHSPHPIQEAARGLGPGLLVVTFDAVFAFLALQIAHVPMVRAF
                     GWLMIVGIVAICISSILNPLAVLGMREYRSPTRSGDFSEGRLGRMVVKMGSLPGSFAL
                     PLAAVSIIVFVGGSIVEPSIKLETDPVNWVNPDSKVVTDLHKVQDEIGGSSELGIFVE
                     STGDGTLFTDDVVSRLDSFSRQQMEEHEKVLLIGTGIINTVSDLTDVDGAAHVAPTAE
                     SVQDAWEIAPDAIKASTVARDGKAINLIYMTRPVPLDRLAGVVDDIREKPDAPAGTRL
                     TPSGLAVVGVGLLQNLEANLVQLTWLAVGLVFAFLVIRLRSLTRGLLSMAPVLVASGA
                     ATLAIWALGISLSPMTAIGGPLVVATCTEFTSLILLRYIEERRRGLEPRAAMDVTAAR
                     TGRAFIVSAMTAVAGVAVISFSSLPLLRNFGIFIALKVTIALLAALIVLPPLIIWADK
                     RGWVSKGMLDHHVEPYLEVADHKAGVGAPTLVGAGVADAPAAPAAPAAPDPAPAAPEA
                     TTGPAAATPPPYSGPPTVPDPGLLPPPYPGPSPDQGPPTQPPPFPGG"
     gene            complement(8161..10242)
                     /locus_tag="PLZ72_05560"
     CDS             complement(8161..10242)
                     /locus_tag="PLZ72_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNA35798.1"
                     /translation="MPNRLADETSPYLRQHADNPVDWWPFGDEAFELARRTDRPVLIS
                     VGYSSCHWCHVMAHESFEDPDTAALINDRFVPIKVDREERPDVDAVYMEATQAMVGRG
                     GWPMTVFADADGRPFFCGTYFPPEPRGGQPGFTQVLDAVHQAWVDRRGDLLEQAERLT
                     ESLRRPVGPAGDDTSEVGRADIDEATMGLLSAFDADWGGFGRAPKFPHTMSLDHLLRQ
                     HHRTGSAAALGAVVTSLDAMAVGGIHDHLGGGFSRYSVDRQWLVPHFEKMLYDNALLL
                     RIYTHAAQVTGEERFAAVAGSIVEYVERDLTHPDGGRYSAEDADSVPAGSAEATHAHE
                     GAFYVWTPEQVATALEAAGLGAHAREVCEWYGITAAGNFEGASIPNRLHARQHPALAP
                     DDRPPAVEAARVALLSDRSRRPRPGLDDKVLTEWNALFVAALAEAGGALGEPDWIAGA
                     ERTARFLLDRLRGPDGRWLRSWQAGPGEADAGRSRHLAYAADHGALLDAFLTLHEVTG
                     HQMWLAEATAVATALDDLFIDDDGTVFSTGSDAPALLVRPREVMDDATPSGASLAAVG
                     FLRLSAHTGDDRWAGRARAVLGALGHLVGRHPLGFGDMLRAVEMTTEGMTELVVSGDR
                     PDLVDVALRAFRPTTVLAWGEPGPGPLWEGRVDDGSDGRAYLCRDHLCEAPITDASEL
                     VRALDAHRRRA"
     gene            10343..10867
                     /locus_tag="PLZ72_05565"
     CDS             10343..10867
                     /locus_tag="PLZ72_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35799.1"
                     /translation="MAKPAELIAAATTVIDAAVAASPDPTAEETVLAAGIFGHALPSN
                     AELAVAGAAADSLPHVGNGLIEAVENVTAMAAVQAMDAERDGLTVRMLVAVTATDIHL
                     LDWQTGRGTTRILRSLGRVSTDVTIKKLGLSRRVELLDHATGTDVHIVGTTAAFSPEA
                     AGDKAVLALLDVPA"
     gene            10934..11683
                     /locus_tag="PLZ72_05570"
     CDS             10934..11683
                     /locus_tag="PLZ72_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA35800.1"
                     /translation="MEIRLDGRVALVTGASKGIGQAIAAEMAAAGASVMLSSRKEGGL
                     RAAAASITPTGGADIDVFAANAGDPEAAAACVSATLERFGGLDILVNNAATNPYMGRM
                     IDIDLPRYDKTYEVNLRGAFVWTQEAYHRAMAERGGCVINIASIGGLSVEVAIGHYNV
                     TKAALIHLTRSLAKELAPGVRVNAICPGLVKTDMARALWESNEDSVAAHVPLRRLGEP
                     SDIAEAAVFLASDRASWITGTTMVVDGGMLL"
     gene            complement(11767..12639)
                     /locus_tag="PLZ72_05575"
     CDS             complement(11767..12639)
                     /locus_tag="PLZ72_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA35801.1"
                     /translation="MPIHETREHPQGSAADPVGLDRVTFDHVAIAVRHPTDPWPIFAD
                     VLGGRYLDRGVNPGFGWTQLRFANGFVLEGLNPEPSDRDDFLNRFLDRNGPGPHHLTF
                     KVPDLDRMLDRVRAAGFDPVGEDRTDPTWYEAFLHPAQAHGIVVQLAQVGPDPHAPAP
                     EPEGFPELTFDHPMASLGRVVHAVADLDGAVSLFRDVLGGRVMSSGAAIDGNHWVELG
                     WNGPGRLRLLEGVHAEIAEWIGDRPGRLRHLFFSFDEPRTVPGVREVAENRWVLDADD
                     VLGTRLVLSSNARP"
     gene            complement(12626..13849)
                     /locus_tag="PLZ72_05580"
     CDS             complement(12626..13849)
                     /locus_tag="PLZ72_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNA35802.1"
                     /translation="MPELVALAVPPGPAFLDALRRIWDDGDAVLPLDPHAPAPHRDRL
                     LNALAPSAVVEADGTRHHRSGGRPVEDGDAVVIATSGTTGAPRGAVHTHAGVAAAARM
                     TSAACGATSSDVGWLACLPLGHVGGFSVITRALLAGTGLEIHDRPDAARIDDAARRGA
                     THVSLVPTLLGRIDPARWRAILLGGSAVPVRRPDNTIATYGMTETFGGVVYDGLALDG
                     VAIRIGSGPDGDSGPEGPIELRSPSLMRAYRDGTDPRTDDGWYRTGDRGSIDAAGVLR
                     VAGRTDDLIITGGVNVWPAPVEDRLRTHPAVADVAVLGVADAEWGQRVRAVVVPSDPR
                     HPPTLDELRSHVKEHLPAAAAPRELLLVDVLPRTALGKISRYELSRRSGPDERSGSDD
                     RRRGTGKAGSPDAHP"
     gene            complement(13842..15083)
                     /locus_tag="PLZ72_05585"
     CDS             complement(13842..15083)
                     /locus_tag="PLZ72_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA35803.1"
                     /translation="MFPDWVWASGEESSDPLVHHPDPDGGGMLTTRVDQLGGSGHLER
                     MDADCRDVAAAGATVLRYGMPWARTEVSPGVYDWSLWDRALAAAEAAGVEVVVDLCHF
                     GLPEHLCGPGSAHRGFTDPAWVDAFLRYVEAFLARYREPRWFTPVNEPTTTAFCSASW
                     GAWNDGVSGAEDYGRALVLCELADALAAAAIRADRPAAFPGAEALQIPAAVHPDRADE
                     ADQQISRWFAALDLRVGHDLDPAAEDLLAGVPDAWLERLAPVADPTGIVAGHDLYPVS
                     VHPYGAVGSDPVELTIEDRVGAWESLARRCAERYGLPIWVAETSNLGLDPADGPAWLA
                     ALAAACGRLRGDGVDVRGICWYSRGDQRDWDTALVPPAGEITRVGLYDMDRRPRPAFD
                     AFRDAATAGAPPPRPGPSPGA"
     gene            15224..15724
                     /locus_tag="PLZ72_05590"
     CDS             15224..15724
                     /locus_tag="PLZ72_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35804.1"
                     /translation="MEQRGVVPIPVPAGPPARILAVLIPVVAVALIAARAARDGLRAS
                     PDDPVSPWIIAVVTVAVGGWLSWRALTQRAELGPDGLSCRNLVTSFEVDWDRVESLIV
                     LRRGPLAMVDLRIRSHRRRLRVGAATRFSDESAEVVLDQIRAHPDAAARLIDPRDDAP
                     LDPHGV"
     gene            15759..16685
                     /locus_tag="PLZ72_05595"
     CDS             15759..16685
                     /locus_tag="PLZ72_05595"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNA35805.1"
                     /translation="MSGSPTSSAADRPSAPAGWRLWVLGARPRTLPAAVAPVLVGTAA
                     APRSELRWWVFLCAMGVAVFVQIATNYANDYSDGRRGTDDPTARVGPPRLVGNGLATP
                     GEVKRAMLVAFGLTALFGTPLVLFVDWRLVFVGVAAIAAGWFYTGGSRPYGYAGFGEV
                     FVFVFFGLVATVGSFYVQALTVTWLPVVAGVALGCLATALLVVNNLRDIPGDTAVGKQ
                     TLAVRLGDRATRGLYVVLMVVPFLLLPIMAGLGERPVAAVAFVALVLARRPVVEVLGG
                     ARGARLIAVLGDTGRVHLVYGLLVAVGFLIGG"
     gene            complement(16767..17591)
                     /locus_tag="PLZ72_05600"
     CDS             complement(16767..17591)
                     /locus_tag="PLZ72_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA35806.1"
                     /translation="MVDPNRTLRSSVVMLHGFAQTGGCLGPVVDALVDHTVVRPDLPG
                     HGGAAALADLDLWGIASHLARAIGPTLDGPAVWFGYSMGGRVALHVALAHPELVSGLV
                     LLGATAGIADAAAREDRRTADLARADRLEAVGLDRFLDEWLAQPLFAGLPEWARFDAE
                     RRRNTVPGLAGSLRHAGTGSMEPLWDRLGTIDVPVLCVVGEHDRKFTDEAHRMADARS
                     GGLPDAAVDVVVGAGHSAHLERPERVAELVVPFCERLAPRTAGRRDADPSPRWYTP"
     gene            17754..19661
                     /gene="menD"
                     /locus_tag="PLZ72_05605"
     CDS             17754..19661
                     /gene="menD"
                     /locus_tag="PLZ72_05605"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNA35807.1"
                     /translation="MSQFPTPTTERPGTPVPGAVDPNDRADPTADPTAGTAAADVAAT
                     FCAALVDEWGRLGVTDAVVSPGSRSTPLALALADSAAVRVHVVHDERSAAFTALGLGV
                     ATRRPALLLCTSGTAAANFHPAVVEAHQAAVPMVVVTADRPPELQGVGAPQTIDQRHL
                     FGSAVRWYCEPGPAAADGAPWWRDLARDSLRRSLGTTPGPVHLNLAFREPLVGTAGTL
                     PPPGTDGGAGADATAVGASGAAWGLVDEELGQTLAAVSNRRGLIVAGARTAVTAADAD
                     AVLALADTLGWPVLADAPSGCRRTHPRVVTTADVLLRHDGFAAAHRPEFVLRLGALHS
                     SRVLGEWLARSGAVQVGIDRHGLVPDPDHVLSWSRPADPAEVCRQLAGARTEPAPAEW
                     TRAWADAEATARRTLDEALGAGGVATEPTTALDALAAVPADGVLVVSSSMPVRDLEWF
                     APARDDVRVVANRGANGIDGVTSTAVGVALTWTPTVLLTGDVAFLHDTNALIGLRRRG
                     VPLVIVVVDNDGGGIFSFLPQADALEPERFEQLFGTPHGLDVAAVAEAHGVAAERVTS
                     RAGLRAAINGALTRGGVRVVAVTSDRSANVAVHRELFSRVAVALDELVTAADPAAGGV
                     NGGGFGNVADR"
     gene            19658..20701
                     /locus_tag="PLZ72_05610"
     CDS             19658..20701
                     /locus_tag="PLZ72_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35808.1"
                     /translation="MSRPGDTSDDHQPTPTGDHEPDIDPDAEFDGPPIVEIAEAIRRE
                     GAPVPRRLGRSVVAEDRALGWAEFGHPDGDVVLWFHGTPGARTQVPPSIHQNALQRGF
                     RVVTVERPGTGTSTDHRYRRIADFAPDVESLVDDLGIGRFAVVGLSGGGPYTLAVARH
                     LPERVVVASLLGGLGPTRGPDAVWSYTRALRFVAPAFEVLRTPIGRVLASGIDLLAPV
                     ADPVFSAYAALLGFADRPVLRDPQFRAMFLQDLLAAHELRSVAHDMALFSRHWGFALD
                     EVTPPVIVWQGLADTIVPPSHGHHQASRLPRAELRVRPGEGHFAGFADVATVLDRLRE
                     VWAQETSEPVTAP"
     gene            complement(20709..>20968)
                     /locus_tag="PLZ72_05615"
     CDS             complement(20709..>20968)
                     /locus_tag="PLZ72_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNA35809.1"
                     /translation="DDAELAVQAELERADRGRYAGPVGWVDAEGNGVFGVGIRGVQIA
                     EDPTSARLFAGVGVVDGSDPWTELEETRSKAAAVLGALTRI"
BASE COUNT         2888 a         7571 c         7536 g         2973 t
ORIGIN      
        1 gcccgccagc catccggctg cgttgttgta gctggcaggt cgcccggcga cctgtgggct
       61 acaagttcgg tgggggaggg ctcgtgggag cggcccggag gacaccgtcg gcgggtcgcg
      121 cgatactcgg aggcggaatc acacgactgt cgtttgtgga ccccgtcgcc ccgctccggc
      181 gcggacggcc gggggtggcg accgtcccgg ggagcgacac agggagcgag aggtgccgga
      241 cagcttcgtc cacctgcacc agcacaccga gtactcgatg ctcgacggcg cggcgcgcat
      301 cggcgacgtc gtcgcctcgg cggtcgcgga cggccagccg gccatgggga tcaccgacca
      361 cggcaacatg tacggggtcc tcgacttcta caaggcctgc cgggactcgg gggtcacccc
      421 gatcatcggc accgaggcct acatggccca cgactcccgc ttcgagcgcc cgccccggcg
      481 gggcaaggtc gacgactccg gcggcgacgt cgagggtggc cgcaagctct actaccacct
      541 gacgctgctg gccgagaacg acgtcggcta ccggaacatc atccagctgg ccagccgcgc
      601 ctacatggag ggctactact acaagccccg ggtcgactgg gacacgctcg ccgagcacca
      661 cgagggcgtc atcgccacca ccggctgcct cggcggccac gtgctgcagt cgctcatgaa
      721 cgagggcttc gacgcggcgc tggccaaggc cgcccgcctc caggacatct tcggacgcga
      781 caacctcttc gtcgagatcc aggaccacgg catccccgag cagcaccgca ccaacccgca
      841 gctgctcgag atcgcccgca agctccaggc cccgctgctc gccaccaacg acagccacta
      901 cgtgcaccgc gacgacgccg tgtcccacga tgcgctcctg tgcgtgcaga ccggctcgct
      961 gatgagcgac ccggaccgct tcaagttcca cagcgacgag cactacctga agaccgcggc
     1021 cgagatgcgc cacctgttcg ccgaggcgcc cgagtcctgc gacaacacgc tgtggatcgc
     1081 cgagcgatgc aacgtcgaga tcgagttcgg caagccgcag ctgcccaact tcccgctgcc
     1141 cgagggcttc accgacgacg ccgagtacct cgagcacctc accttcgagg gcgcccgcaa
     1201 gcgctggggc gaccagctgc ccgacaacgt cgtcgaacgc ctggcctacg agctcaaggt
     1261 catcggcgac atggggttca gctcctactt cctgatcgtg tgggacctga tccgccacgc
     1321 ccgcgacgcc aacatccgcg tcggtcccgg ccgtgggtcc gcggcggggt gcgcggtcgc
     1381 ctacacgctg tggatcaccg acctcgaccc catcaagtac gacctgctgt tcgagcggtt
     1441 cctcaacccc agccgcatct ccatgcccga catcgacatg gacttcgact cccgctaccg
     1501 cgacgagatg atccgctacg ccgcggagcg atacgggcgt gaccacgtgg cgcagatcgt
     1561 caccttctcg acgatcaagg cccgggccgc ggtgcgcgac gcggcgcggg tgctcggaaa
     1621 gccctacatc gtgggcgaca aggtcgccaa ggccatgccg ccgctggtga tggggcgcga
     1681 caccccgctg tacgcctgtc tggaggagca ccccaagttc gtcgacggct acaagatggc
     1741 cgccgagctg cgcgagatgt acgcgaccga ccccgagacc agggaggtcg tcgacgtcgc
     1801 caagggcctc gaggggctgc gacgccagga cggcatccac gccgccgcgg tggtgatcac
     1861 caaggagccg ctgaccgagt acctgccgat ccagcgcaag cccgagtccg gcgtgccgat
     1921 cgaagaggca ccggtcgtca cccagtacga gatgcacggg gtcgaggagc tcggcctgct
     1981 caagatggac ttcctgggcc tgcgcaacct cgacgtcatc tccgacacgc tcgagctgat
     2041 ccggcgcacc cggggagtcg aggtcgacat cgacgacgtc gacctgaccg acgaacccac
     2101 ctacgagctg ctgcgtcggg ccgacacgat cggcgtgttc cagctggagg gcggcgccat
     2161 gcgggccctc atccggtcgc tggcgccgac cgagttcgag gacgtcgccg ccctcgtcgc
     2221 cctgtaccgg cccggtccga tggcggcgaa catgcacacc gactacgcgg accgtaagaa
     2281 cggccggcag gaggtcacct acctgcaccc cgacgccgag gagatcctcg gcgacaccta
     2341 cgggttgtgc atctaccagg aagggatcat gcggatcgcc cagcgcttcg cgggctactc
     2401 cctggccgac gccgacaacc tccggaaggc gtgtgggaag aaggtgcgtg agctcatcgc
     2461 caaggagcgc accaagttcg tcgagggttg cgaggccacc ggctacggcg ccgagctggg
     2521 caccgcctgg ttcgacatca tcgagccctt cgccgactac gcgttcaaca agagccacag
     2581 ctacggctac ggcttcgtcg cgtaccagac cgcctacctc aaggcgaact accccgccga
     2641 gtacctctct gcgcttctga ccagcgtcaa gaccaacctc gacaaggccg cggtgtacct
     2701 ggccgagtgc cgggccatgg gcatcgaggt caccgtcccc gacgtcaacc gctcgctgtc
     2761 ggacttcgct cccgtggtgc ccgaacccga cggcgacggc gcgaacctcg acgggggagc
     2821 ggaccggatc ctcttcggcc tgtccgcggt ccgcaacgtc ggcaccggcc tggtcgagct
     2881 catcatcgag gagcgggacg agaacggccc cttcgaggac ttctacgact tctgccaacg
     2941 ggtcaacacc caggtgctca acaagcgcac catcgaatcg ctgatcaagg cgggcgggtt
     3001 cgactcgatg ggtcaccccc gtcagggtct gctcaccgtg ttcgagcaga tcgtcgacac
     3061 gaccatcgcc cgtcgcaagg agcacgacat gggcgtcatg tcgctcttcg gcgagatgga
     3121 ggagggaccc gcgttcgacg agcgggtccg catccccgac gtcgagttcg acaagaagca
     3181 caggctcgcg ttcgagaagg agatgctcgg cctctacgtg tcggaccatc cgctgctcgg
     3241 cgccgagcag gccctcaagc gcaaggtcga ctgcacgatc ctcgagctcg gcgacctgga
     3301 cgacggcacc cagcgcacgg tcggcggggt catcaccggc ctgcagcgca agtggaccaa
     3361 gaagggcgac ctgatggccg tgttcgtcct cgaggacctg cagggctcgg tcgaggtgat
     3421 ggtgttcccc aagaccatgg cccagatcgg tcacctgctg gtcgaggacg cggtggtgat
     3481 caccaccggt cgggtcgaca agcgcgacga gaccgccaag ttcatccccc gcgagctcga
     3541 gctgttcgaa ccgcaggtcg acgagcaccc gccgctgcgg ctgcacctgc cgccgtcgcg
     3601 cctcgacaac gccacgatcg accggctcca ggagctgatc ggcgacttcc ccggcgactc
     3661 cgaggtgcac atcctgctgg gcgacaagca ggtgctgcgc ctctccgacg accacctggt
     3721 cgacacccgc acgggcctta tcggtgagct gcgagcgctg ctcggccacg aatcggtggt
     3781 gctgtgagcc gttccgggac cgaccccgga cgcgggtcgc gacgcatggc tgaccgcgga
     3841 tctgccacag ccgaccgccg ggcctgactt gccgaatggg cgtcggggcg actgaactag
     3901 tgggaccact cgtgtaccca gaaccgggtc cgcgtccccg aagcgggatg tgacgacacc
     3961 tcagggagaa gatgcatggc gctcgacgtc acgaccaagg actgcaccgc tctgagtgat
     4021 gcggaactgg ccgagatggc cgacctctgc gccgacgggg gcatctgcta cgagatcggg
     4081 ctcctgtcga agctggtcag cgactgggtg ctggtcaccc aggtccggga gggggcgcac
     4141 ctccgggcgt tctcgttctc gaccctcgag cggatcggcg gaactccgag cgtgctgatc
     4201 ggggtggccg gcatccggcg cacctccaag cgcgacagct tcctgcgggc gatcgtgagc
     4261 gacaacctgc gccgggcgct gatggcgttc cccgatgagg acgtgctggt cggcacccgc
     4321 atggtcgacc ccggtgcatt cgaggcctac aaggtcatgg tcgacatcgt cccccgtccg
     4381 ggccacaagg tgtccggaga ggagcgggcc tggggtcgtc gcctggccaa gcgattcgag
     4441 gtcgacgccg agcgctacga ccagcacacc ttcaccgtca ccggtgacgg ggcggcgccg
     4501 ttggtgctcg accacgagcc gctcaagccg gagaaggtcg acccggacgt cgccgcgcag
     4561 ttcacccatc tcgagaccga tcgaggggac tgcctgatca ccttcggctg ggtgatggcc
     4621 gaggagctcc tcaagtacaa cgggtgaccc gagtcgaccc cgaccctgcc agccccgacc
     4681 ctgccgaccc cggcgggcgg gccgccttct cccaggtcct cgcgtcgcgg cgcatgtgcc
     4741 gcgacttcaa acccgacccg gtcgccgacg acgtcctcga ccgggtcctg gccgcggcgt
     4801 tccgggcccc ggccgccggc aacaccgacg cgttggacct ggtcgtgctc gccggcgcgg
     4861 acaccgcccg ccactgggac gtcaccttgc ccgccgatcg ccgacccggg ttccgctggc
     4921 ccgggttgat gcgggcgccg gtgctcgtcg aggtgatcgt cgacccggcc gcctacgtcg
     4981 agcgctactc cctgcccgac aaggctcaca gcggcctggg cgacggagcg gaggcgtggc
     5041 cggtgccgtt ctggttcgtc gacggcggtg ccgcggtcat ggccgtgctg ctggccgccg
     5101 agtccgaagg gctgggagcg ctgctgttcg gtgtcttcgg ccacgaagag gcggtccggc
     5161 gggccctcgg agtgcccgcc gggcgacggg tcgtggccac cgtcgccctc ggtctccccg
     5221 cgccgggagg ccgactcccg tcacggtcgg cgcgccgagg tcgaccgggt cccgatcgtc
     5281 gcgtccaccg gggcgggtgg gctggacagg gtccggctcc gtcgggctag cgtcgccgtt
     5341 cgactgggga caggtgcggt tcaccacgtt ccgctcgaga gccgagggag acaactccga
     5401 tgatgcagcg cttctggtcc tggctggcgg tcaacctggg caagcacgcg gtgatcgtga
     5461 ccgcggtgct ggccgccttc acgatcctgg tcggcaccgg catcacccgc ctcgagttcg
     5521 ccaccggcca ggacagctac ctgaacgcga aggatcaggt ctacaaggac aacgtcgtct
     5581 accagaagct gttcggtggc gaagcgatgc tgtcggtcat cacgatggac gagggccaca
     5641 ccgtcgatga gctgttcact ccggaagggc ttgcgacctt ccagagcgtc accgaggaga
     5701 tcgaggggat cgagggcgtc cagggtgtga tctccccggt caccgccctg cagttctcgt
     5761 ccgatctcgt gacgccgccg gagggcggca cgccgttcga ctcgatcgcg gccaaggcgc
     5821 tgctgcacgc gagcgaggtc gcggagaagt ccggggacac cgccagtgcc caagcccgga
     5881 gcgccgacac ggcggcgacc gcgggccgac tcctcgcggt gccggccgac caacaggtgc
     5941 tctccaaccc cgcgtgggtg aagttcctcc tgctcgacaa caacgaccag gtccgcaagg
     6001 cgctcctccc gttcttcccc gacaagaccc acgccctgat gatcacccgg ctcgacggca
     6061 acatgtcgat cgagcaggag ggcacgacct ccgacgcggt ggtcgcggcg accaaggacc
     6121 tccagttccc gaactcgtcg acggtcacca cgggtgcgcc gatcctgctg aagaacatca
     6181 acgactacct gaagggcggg atgctgaccc tcgggggcat cgcggtgctc gcgatgatcc
     6241 tgatcctgct gctgttcttc agcgtgcgtt ggcggctgct gccgctcgcg gtggtgctgg
     6301 tcggtgtcgt gtgggcgttc ggcttcgccg gctacctcgg catcccgctc acgctggtga
     6361 ccatcgccgg tctgccggtc atgttgggca tcggcatcga ctacgcgatc cagatgcact
     6421 cgcggatcga ggaggaggtg atcatcgccc actcgccgca cccgatccag gaggccgctc
     6481 gcgggctcgg gccgggtctg ctcgtcgtca ccttcgacgc cgtgttcgcg ttcctcgcgc
     6541 tgcagatcgc tcacgtgccg atggtccgtg cgttcggctg gctgatgatc gtcggcatcg
     6601 tcgccatctg catcagctcg atcctcaacc cgctcgcggt cctgggcatg cgggagtacc
     6661 ggagtccgac gcgctcgggt gacttcagcg agggccgtct cggtcggatg gtcgtcaaga
     6721 tgggcagcct gcccggcagc ttcgccctgc cgctcgcggc cgtgtcgatc atcgtgttcg
     6781 tcggcgggag catcgtcgag ccgtcgatca agctcgagac cgacccggtc aactgggtca
     6841 accccgacag caaggtcgtc accgacctcc acaaggtgca ggacgagatc ggcgggtcct
     6901 cggagctcgg catcttcgtc gagtcgacgg gcgacggaac gctgttcacc gacgacgtcg
     6961 tgtcccggct ggactcgttc agccgacagc agatggagga acacgagaag gtcctgctga
     7021 tcggcaccgg gatcatcaac accgtgtcgg acctgaccga cgtcgacggc gcggcgcacg
     7081 tcgccccgac cgccgagtcg gtgcaggacg cgtgggagat cgcccccgac gcgatcaagg
     7141 cctcgaccgt cgcccgcgac ggcaaggcga tcaacctcat ctacatgacc cggcccgttc
     7201 cgctcgaccg cctcgccggt gtcgtcgacg acatccgaga gaagcccgac gccccggccg
     7261 ggacgcggct cacgccgtcg ggtctcgccg tggtcggcgt cggactgctc cagaacctgg
     7321 aggcgaacct ggtccagctg acctggctgg ccgtcggact cgtgttcgcc ttcctcgtca
     7381 tccggctccg ctccctcacc cggggcctgt tgtcgatggc gccggtgctc gtcgcgtcgg
     7441 gagcggcgac gctggcgatc tgggcgctcg ggatctcgtt gagcccgatg acggcgatcg
     7501 gcggcccgct cgtcgtcgcg acctgtaccg agttcacctc cctgatcctg ttgcgctaca
     7561 tcgaggagcg gcgccggggc ctggagccgc gggcggcgat ggacgtgacc gcagcacgga
     7621 cgggtcgggc cttcatcgtc tcggccatga ccgcggtcgc tggtgtggcg gtgatctcgt
     7681 tctcgtcgct gccgttgctg cgcaacttcg gcatcttcat cgccctcaag gtcaccatcg
     7741 ccctgctggc cgcgctcatc gtgctgccgc cgctgatcat ctgggccgac aagcgggggt
     7801 gggtgtccaa gggcatgttg gaccaccacg tcgagcccta cctcgaggtc gccgaccaca
     7861 aggcaggggt cggtgcgccc accctggtcg gcgcgggggt ggccgatgct ccggccgctc
     7921 cggccgctcc ggcggcaccg gatcccgcac cggccgcgcc ggaggcgacg acgggcccag
     7981 ccgccgccac cccgccgccg tactccgggc cgcccacggt cccggacccg gggctgctgc
     8041 cgccgccgta cccgggcccg tcgcccgatc agggtccgcc cacccagccc ccgccgttcc
     8101 cgggcggctg accgcccggg ctccggcggg tcgcgatgac ccgggcccgg gtgggtccgc
     8161 tcaggcccga cgccggtggg cgtcgagcgc ccggacgagc tcggacgcgt cggtgatcgg
     8221 cgcctcgcac aggtggtccc ggcacaggta ggcccgtccg tcgctcccgt cgtcgacccg
     8281 gccctcccac agggggccgg gtccgggctc cccccaggcc agcaccgtcg tgggccggaa
     8341 cgcgcgcagg gcgacgtcga ccagatcggg gcgatcgccc gacacgacca actcggtcat
     8401 gccctcggtg gtcatctcca ccgctcggag catgtcgccg aagccgagcg ggtggcggcc
     8461 gaccaggtgg cccaacgccc cgagcacggc ccgggcgcgg cccgcccagc ggtcgtcgcc
     8521 ggtgtgggcc gacaggcgga ggaagccgac cgcggccagg ctcgcccccg acggggtggc
     8581 gtcgtccatg acctcgcggg gccgcaccag cagcgccggg gcgtcgctgc cggtcgagaa
     8641 gacggtgccg tcgtcgtcga tgaacaggtc gtcgagagcc gtggccaccg cggtcgcctc
     8701 ggccagccac atctgatgac cggtcacctc gtgcagggtg aggaacgcgt ccaggagcgc
     8761 tccgtggtcc gcggcgtagg ccaggtgccg ggaccggccc gcgtcggcct cgcccggccc
     8821 ggcctgccag ctccgcagcc accgcccgtc ggggcctcgg agccggtcga gcaggaaccg
     8881 ggcggtccgt tccgccccgg cgatccagtc aggctctccg agggcaccgc ccgcttcggc
     8941 cagagcggcc acgaacaggg cgttccactc ggtcaggacc ttgtcgtcga ggccggggcg
     9001 gggccggcgg gaccgatcgg agagcagagc gacccgggcg gcctccaccg cgggcggtcg
     9061 atcgtcgggt gccagcgcgg ggtgctggcg ggcgtgcagc cggttgggga tcgacgcccc
     9121 ttcgaagttg ccggcggcgg tgatcccgta ccactcgcac acctcccggg cgtgggcgcc
     9181 gagtccggcg gcctcgaggg ccgtggcgac ctgctcgggc gtccacacgt agaacgcgcc
     9241 ctcgtgggcg tgggtggcct ccgccgagcc cgccggcacc gagtcggcgt cctcggcgga
     9301 gtagcgccct ccgtcggggt gggtcaggtc acgctccacg tactcgacga tcgatccggc
     9361 caccgcggcg aagcgctcct caccggtgac ctgcgcagcg tgggtgtaga tgcgcagcag
     9421 cagggcgttg tcgtagagca tcttctcgaa gtgcgggacc agccactgcc ggtccaccga
     9481 gtagcggctg aagccgccgc cgaggtggtc gtggatgccg ccgaccgcca tggcgtcgag
     9541 ggacgtgacc acggctccga gcgcggcggc cgaaccggtg cggtggtgct ggcgcagcag
     9601 gtggtccagg ctcatcgtgt gggggaactt gggggcgcgt ccgaatccgc cccagtcggc
     9661 gtcgaaggcg gagagcaggc ccatcgtggc ctcgtcgatg tcggcccgac cgacctcgga
     9721 cgtgtcgtcg ccagcggggc cgaccgggcg gcgcaacgac tcggtcaggc gttccgcctg
     9781 ctccagcagg tcgccacgcc gatcgaccca ggcctggtgc accgcgtcga gcacctgcgt
     9841 gaagccgggc tggcctcccc gcggttcggg tgggaagtag gtgccgcaga agaacgggcg
     9901 accgtcggcg tccgcgaaca ccgtcatcgg ccagccgccc cggccgacca tggcctgggt
     9961 ggcctccatg tagaccgcat cgacgtcggg gcgctcttcg cggtcgacct tgatcggcac
    10021 gaagcggtcg ttgatcagcg ccgcggtgtc ggggtcctcg aaggactcgt gggccatcac
    10081 gtggcaccag tggcacgacg agtagcccac cgagatcaga accggccggt cggtgcgtcg
    10141 ggcgagctcg aacgcctcgt cgccgaacgg ccaccagtcg accgggttgt cggcgtgctg
    10201 acgcaggtac gggctggtct cgtccgcgag gcggttcggc atggcggcaa cctacgtggt
    10261 gggtcgacgt gtgcggtgat cgccgacgga tccgtggagc ctgcggggtt ggaacgcggg
    10321 cccgaccccg gcaggctggg ggatggccaa acccgctgag ctcatcgccg ccgccaccac
    10381 cgtgatcgac gcggcggtcg ccgcgtcgcc ggatccgacc gccgaggaga cggtgctcgc
    10441 cgccggcatc ttcggtcacg ccctgccgtc gaacgccgaa ctggccgtgg ccggagcggc
    10501 ggccgacagc ctcccccacg tcggcaacgg gttgatcgag gccgtggaga acgtgaccgc
    10561 catggccgcg gtgcaggcga tggacgccga gcgcgacggg ctcaccgtcc ggatgctggt
    10621 cgcggtgacc gccaccgaca tccacctgct cgactggcag accggccgcg gcaccacccg
    10681 cattctgcgg agcctcgggc gcgtgagcac cgacgtgacc atcaagaagc tgggtctgag
    10741 ccgacgtgtg gagctgctcg accacgccac cggcaccgac gtccacatcg tgggcacgac
    10801 cgctgcgttc agccccgagg cggccggcga caaggccgtc ctggccctgc tcgacgtgcc
    10861 ggcctgaaac caccgggacc cgcccccggg agcggtccgg tacccggtga agggcgccgc
    10921 gtagcgtggc gccatggaga tccgactgga cggacgggtg gctctcgtca cgggagcctc
    10981 gaaggggatc gggcaggcga tcgcggccga gatggccgcg gcgggtgcgt cggtcatgct
    11041 gtcgtcgcgc aaggagggcg gcctgcgcgc tgccgccgcg tcgatcacgc cgaccggtgg
    11101 cgccgacatc gacgtgttcg ccgccaacgc cggtgacccc gaggcggccg ccgcctgcgt
    11161 gtccgccacc ctcgagcgct tcggcggcct cgacatcctg gtcaacaacg cggcgaccaa
    11221 cccgtacatg ggccgcatga tcgacatcga cctgccccgg tacgacaaga cctacgaggt
    11281 caacctgcgc ggggcgttcg tgtggacaca ggaggcctac caccgggcga tggccgagcg
    11341 aggtggatgc gtgatcaaca tcgcctcgat cggtggcctg tcggtcgagg tggcgatcgg
    11401 tcactacaac gtgaccaagg ccgctctcat ccacctcacc cgttctctgg ccaaggagct
    11461 cgcgcccggg gtccgggtca acgccatctg cccgggactg gtcaagaccg acatggcccg
    11521 ggccctgtgg gagtcgaacg aggactcggt ggccgcgcac gtgccgttgc gtcgcctcgg
    11581 cgaaccgtcc gacatcgccg aggccgcggt gttcctggcc tcggacaggg cgtcgtggat
    11641 caccgggacg accatggtcg tcgacggtgg gatgctcctc tgacggtggc acggcgttcc
    11701 ggccgacgag ccggccgacg atccggccga ggagccggct gatgagcagg ctgatgggct
    11761 gaccggtcag gggcgggcgt tggacgacag caccagtcgg gtgcccagca cgtcatccgc
    11821 gtcgagcacc cagcggttct cggccacctc gcggaccccg ggaaccgtcc tcggctcgtc
    11881 gaagctgaag aacaggtggc gcagccgacc cggtcggtcg ccgatccact cggcgatctc
    11941 ggcgtgcacg ccctcgagca gacgcagacg gccgggaccg ttccagccga gctcgaccca
    12001 gtggttgccg tcgatcgcgg cgcccgagga catgacccgt cctccgagca cgtcgcggaa
    12061 caggctcacc gccccgtcca ggtcggccac ggcgtggacg acccgcccca gagaggccat
    12121 cgggtggtcg aaggtcagct cggggaaccc ctcgggctcg ggagccggtg cgtgggggtc
    12181 ggggccgacc tgcgccagct gcacgacgat cccgtgggcc tgggcggggt gaaggaacgc
    12241 ctcgtaccag gtcgggtccg tccggtcctc gcccaccggg tcgaaccccg cggcgcggac
    12301 ccggtcgagc atgcggtcga ggtcggggac cttgaaggtc aggtggtggg gtccgggccc
    12361 gttgcggtcg aggaaccggt tcaggaagtc gtcccggtcg ctcggctcgg ggttgaggcc
    12421 ctcgaggacg aacccgttgg cgaagcggag ctgcgtccag ccgaagccgg ggttgacccc
    12481 ccgatcgagg tagcggccac cgagcacgtc ggcgaagatc ggccacgggt cggtcgggtg
    12541 ccggacggcg atcgcgacgt ggtcgaacgt gacgcggtcc agcccgacgg ggtcggcggc
    12601 ggagccctgc gggtgctcgc gcgtctcatg gatgggcatc gggtgatccc gcctttccag
    12661 tgcccctgcg gcggtcatcg gatccggatc gttcgtcggg tccggagcgt cggctcagtt
    12721 cgtacctcga gatcttgcca agcgcggtgc gtgggagcac gtcgacgagc agcagctccc
    12781 ggggtgcggc cgcggcgggc aggtgctcct tcacgtgcga gcggagctcg tccagggtcg
    12841 gcgggtggcg tgggtcgctg ggcacgacca cggcccggac ccgctggccc cactcggcgt
    12901 cggccacgcc cagcaccgca acgtcggcta ccgcagggtg ggtgcggagg cggtcctcga
    12961 ccggagcggg ccacacgttg accccgccgg tgatgatcag gtcgtcggtc cgcccggcca
    13021 cgcgcagcac gccggcagcg tcgatggagc cccggtcgcc ggtgcggtac cagccgtcgt
    13081 cggtgcgggg atcggtcccg tcgcggtagg cccgcatgag cgacgggctc cgcagctcga
    13141 tcggcccctc ggggccggag tcgccgtcgg ggccggatcc gatccggatg gcgacgccgt
    13201 cgagtgcgag accgtcgtag acgacgccgc cgaaggtctc ggtcatcccg taggtggcga
    13261 tcgtgttgtc cggacgacgg accgggacgg cggagccgcc gaggaggatc gcccgccacc
    13321 gtgccggatc gatccgtccg aggagggtgg ggaccagcga cacgtgggtg gcgccgcgcc
    13381 gggcggcgtc gtcgatgcgc gccgcgtcgg ggcggtcgtg gatctcgagt ccggtccccg
    13441 cgagcagcgc ccgggtgatg accgagaagc cgccgacgtg gccgagcggc aggcaggcca
    13501 gccagccgac gtcgctgctg gtggctccgc aggccgcaga ggtcatcctg gccgcggcgg
    13561 cgaccccggc gtgggtgtgg accgccccgc ggggagctcc ggtggtgccc gaggtggcga
    13621 tgaccacggc gtcgccgtcc tcgacgggcc gccccccgct ccggtggtga cgtgttccgt
    13681 cggcctcgac gaccgcggat ggcgcgagcg cgttcaggag ccggtcccgg tgcggagccg
    13741 gcgcgtgcgg atcgaggggg agcacggcgt cgccgtcgtc ccagatccgc cggagcgcgt
    13801 cgaggaacgc cggcccgggc ggcacggcga gagcgaccag ctcaggcacc gggggatggt
    13861 ccgggtcgtg gcggtggcgc tccggcggtg gccgcgtcgc ggaacgcgtc gaacgccggg
    13921 cggggccggc ggtccatgtc gtagaggccg acgcgggtga tctcgcccgc cgggggcacc
    13981 agggcggtgt cccagtcgcg ctggtcgccc cggctgtacc agcagatgcc ccggacgtcg
    14041 acgccgtcgc ctcgcagtcg tccgcacgcc gcggccaggg cagcgagcca ggccggtccg
    14101 tcggcggggt ccagtcccag gttggacgtc tcggcgaccc agatcggcag cccgtagcgc
    14161 tccgcacagc ggcgggcgag gctctcccac gcaccgacgc ggtcctcgat cgtcagctcg
    14221 accgggtcgg accccaccgc gccgtacggg tggaccgaca ccgggtagag gtcgtggccg
    14281 gccacgatgc cggtcgggtc ggccaccggg gccagtcgct ccaaccatgc gtcggggacc
    14341 ccggcgagca ggtcctcggc cgccgggtcg aggtcgtggc cgacgcgcag gtcgagcgcc
    14401 gcgaaccacc gcgagatctg ctggtcggcc tcgtcggccc gatccgggtg caccgcggcg
    14461 gggatctgca acgcctcggc gccggggaac gccgccggcc ggtcggcccg gatcgccgcc
    14521 gcggccaggg cgtcggccag ctcgcacagc accaacgcgc ggccgtagtc ctcggcgccg
    14581 ctgaccccgt cgttccacgc cccccacgac gccgagcaga acgccgtggt ggtcggctcg
    14641 ttgaccgggg tgaaccagcg gggctcgcgg tagcgggcca ggaaggcctc gacgtagcgg
    14701 aggaacgcgt cgacccacgc cgggtcggtg aagcccctgt gcgccgatcc ggggccgcac
    14761 aggtgctcgg gcaggccgaa gtggcacagg tcgaccacga cctcgacccc ggcggcttcc
    14821 gcggcggcca gggcccggtc ccacagcgac cagtcgtaga cgccggggga cacctcggtg
    14881 cgcgcccacg gcatcccgta gcgcagcacg gtcgcgccgg ccgcggcgac gtcgcggcag
    14941 tcggcgtcca tgcgctccag gtggccgctg ccgccgagct ggtcgaccct ggtcgtgagc
    15001 atccccccgc cgtcggggtc tgggtggtgc accagcgggt ccgaggactc ctcgcccgat
    15061 gcccagaccc agtcggggaa caccacctcg gcggtgccgg gtgtcgggtc gggttcgggg
    15121 tcgggtctca gggtggtctc cggtgaggcg tgcagcgtga ggcggtcggg ggtggcgtcg
    15181 gcgcggatcg cggcgcggtc aggaacggta gcgtcgtcgc ccgatggagc agcgcggcgt
    15241 cgtcccgatc ccggtcccgg cggggccgcc ggcgcggatc ctggccgtgc tcatcccagt
    15301 ggtcgcggtg gcgctgatcg cagcgcgggc tgctcgggac ggactcagag cctcccccga
    15361 cgatccggtg tcgccgtgga tcatcgccgt ggtgaccgtc gcggtgggtg ggtggctgag
    15421 ctggcgggcg ctgacccaga gggccgagct cggacccgat ggcctgagct gccgcaacct
    15481 cgtcacctcg ttcgaggtcg actgggaccg ggtcgagtcg ctgatcgtcc tgcgccgggg
    15541 tccgctggcg atggtcgacc tgcggatccg ctcgcaccgt cgccggctgc gggtgggggc
    15601 agccacacgg ttctccgacg agtccgccga ggtggtcctc gaccagatcc gggcccaccc
    15661 cgatgctgcg gcccgactga tcgacccgcg cgacgacgca cctctggacc cgcacggcgt
    15721 ctgagccccg ccgcggtccg tcggtagcat cggccgccgt gtccggatcc ccgacctctt
    15781 ccgctgccga ccggccgtcg gcgcccgccg gctggcggct ctgggtgctc ggggcccgac
    15841 cccgcaccct tcccgccgcg gtggccccgg tgctggtcgg caccgcggcg gccccccgga
    15901 gcgagctgcg ctggtgggtg ttcctctgcg ccatgggcgt ggcggtgttc gtgcagatcg
    15961 ccaccaacta cgccaacgac tacagcgacg gtcggcgcgg caccgacgac ccgacggccc
    16021 gggtcggccc accccgactg gtcggcaacg gcctggccac cccgggcgag gtcaagcggg
    16081 cgatgctcgt cgcgttcggg ctgaccgcgc tgttcggcac gccgttggtg ctgttcgtgg
    16141 actggcgcct ggtgttcgtc ggcgtggccg cgatcgccgc cgggtggttc tacaccggag
    16201 ggtcccggcc ctacggctac gccgggttcg gcgaggtgtt cgtcttcgtg ttcttcggcc
    16261 tggtggcgac cgtcgggtcg ttctacgtgc aggcgctcac ggtgacgtgg ttgccggtgg
    16321 tcgccggcgt cgcgctcggt tgcctggcga ccgcgctgct ggtggtgaac aacctccgcg
    16381 acatccccgg cgacaccgcg gtgggcaagc agaccctggc ggtgcggctc ggggaccggg
    16441 ccacccgagg gctctacgtg gtcctgatgg tcgtgccgtt cctgctgctt ccgatcatgg
    16501 ccgggctggg ggagcggccg gtggccgcgg tggcgttcgt cgcgctggtg ctggcccgtc
    16561 gacccgtggt cgaggtcctc ggcggcgccc gtggcgcccg gctcatcgcc gtcctcggcg
    16621 acaccggccg ggtccatctc gtgtacgggc tgctggtcgc cgtcggcttc ctgatcggcg
    16681 gctgaacccc gcccccaagg tttctgacga cactggagta ccgccacggt gctgacttgt
    16741 cgtcagaacc cggggtgggg gtgcggtcag ggggtgtacc agcgggggga ggggtcggcg
    16801 tcgcgtcggc cggcggtgcg gggcgccagt cgctcgcaga acgggacgac gagctcggcc
    16861 acccgctccg gccgttcgag gtgcgcggag tgccctgcgc ccaccacgac atccaccgcg
    16921 gcgtccggga ggccgccgga gcgggcatcg gccatgcgat gggcctcgtc ggtgaacttc
    16981 cggtcgtgct cgccgacgac gcacagcacc gggacgtcga tggttccgag ccggtcccac
    17041 agcggctcca tggagccggt cccggcatgg cgcaggctcc cggccagccc ggggacggtg
    17101 ttgcggcgcc gctcggcgtc gaagcgggcc cattcgggca gacccgcgaa cagcggttgg
    17161 gccaaccact cgtcgaggaa tcggtcgagg ccgaccgcct cgaggcggtc ggcccgggcc
    17221 aggtccgcgg tccgtcgatc ctcgcgggct gcggcatcgg cgatgccggc tgtcgctccg
    17281 aggaggacga gtccggacac cagctcgggg tgcgcgagcg ccacgtgcag cgccacccgg
    17341 ccccccatcg agtagccgaa ccacacggcg ggcccgtcga gcgtcggtcc gatcgcccgc
    17401 gccagatgcg aggcgatgcc ccacaggtcg agatcggcca gcgcggccgc gccaccgtgg
    17461 ccggggaggt cgggtctgac gacggtgtgg tcgacgaggg cgtcgacgac agggccgagg
    17521 cagccgccgg tctgggcgaa gccgtgcagc atgaccaccg acgagcgcag ggtccggttc
    17581 gggtcgacca cgcctcccac cgtaggctgg cccgtcgccg tcggcggccc cgggtccgac
    17641 ccgtggagcc cgacccgcag cccgcgtccg gccgagcaca cctcgtcacc ggccccatcc
    17701 cacgacccga gcgatccccg accatgtccc agtccccgat atcccagtcc ccgatgtccc
    17761 agttcccgac tccgacgacc gaacgacccg gcaccccggt cccgggcgcg gtcgatccga
    17821 acgaccgggc cgacccgacc gccgacccga ccgccgggac ggcagccgct gacgttgccg
    17881 ccacgttctg cgccgcgctg gtcgacgagt ggggccgcct cggcgtcacc gacgccgtcg
    17941 tctcgccggg ttcgcgctcg acgccgctgg ccctggcgct ggccgacagc gccgctgtgc
    18001 gcgtccacgt cgtgcacgac gaacgctccg ccgcgttcac ggcgctcggg ctgggtgtcg
    18061 ccacccgacg tcccgctctg ctgctgtgca ccagcggcac cgcggcggcg aacttccacc
    18121 ctgcggtggt cgaggcccac caggccgcgg tgccgatggt cgtcgtcacc gccgaccgcc
    18181 cgccggagct ccagggggtc ggcgcgccgc agacgatcga ccagcgccac ctgttcggct
    18241 ccgcggtgcg ctggtactgc gagccgggac ccgccgcggc cgacggtgct ccgtggtggc
    18301 gtgacctcgc ccgcgactcg ttgcgccgtt cgctcggcac cacccccgga ccggtccacc
    18361 tgaacctggc gttccgcgag cccctggtcg gcaccgccgg aaccctgccc ccgccgggta
    18421 ccgacggcgg tgccggcgcc gacgcgacgg cggtcggagc ttcgggagcc gcgtggggcc
    18481 tggtcgacga ggagctgggt cagacgctcg ccgccgtctc gaaccggcgc ggcctcatcg
    18541 tcgccggtgc ccgcaccgcg gtgaccgcgg cggacgccga cgcggtgctg gccctggccg
    18601 acaccctggg ttggcccgtg ctcgcagatg ctccgtcggg ctgtcgacga acccatccgc
    18661 gggtggtgac gaccgcggac gtcctcctgc gccacgacgg gttcgcggct gcccaccgac
    18721 ccgagttcgt gctccgcctc ggggcgttgc actcgtcgcg ggtcctgggc gagtggctgg
    18781 cccgcagcgg tgcggtccag gtcggcatcg accgccacgg cctggttccc gaccccgacc
    18841 acgtgctgtc gtggtcccgt ccggccgatc ccgccgaggt gtgccgccaa ctcgccggtg
    18901 cccgaaccga gccggctccg gcggagtgga cccgggcgtg ggcggatgcc gaggcgacgg
    18961 cccgtcggac cctcgacgag gccctcggcg ccggcggtgt tgccacggaa cccacgaccg
    19021 cactcgacgc cctggcagcg gtgcccgccg acggtgtgct ggtggtgtcg tcgtccatgc
    19081 cggtgcggga cctcgagtgg ttcgcccccg cccgcgacga cgtccgcgtc gtggccaacc
    19141 ggggcgccaa cggcatcgac ggcgtcacct cgaccgcggt cggcgtggcg ctcacctgga
    19201 cgccgaccgt gctgctcacc ggtgacgtcg cgttcctcca cgacaccaac gccctgatcg
    19261 gcctgcgccg tcggggcgtc cctctggtga tcgtggtggt ggacaacgac ggcggcggca
    19321 tcttcagctt cctgccccag gccgacgcgc tcgaaccgga acgcttcgaa cagctcttcg
    19381 ggaccccgca cgggctcgac gtcgcggcgg tggccgaggc ccacggagtg gccgccgaac
    19441 gggtcaccag ccgagccggg ttgcgcgccg cgatcaacgg cgcgctcaca cggggaggcg
    19501 tgcgggtggt ggccgtcacc tcggaccggt cggccaacgt ggcggtgcac cgggagctgt
    19561 tctcccgggt cgcggtcgcg ctggacgagc tcgtcaccgc agcggacccg gcggccggtg
    19621 gggtgaacgg cggcggtttc ggtaacgtcg cggaccgatg agcaggccgg gcgacacctc
    19681 cgacgaccac cagccgaccc cgacgggcga ccacgagccc gacatcgatc ccgatgccga
    19741 gttcgacggc cctccgatcg tggagatcgc cgaggcgatc cgacgcgagg gagcaccggt
    19801 gccccgccgt ctcggacgca gcgtcgtcgc cgaggaccgg gcgctcggat gggccgagtt
    19861 cggacatccc gacggcgatg tcgtcctctg gttccacggc acgcccggcg cgcgcaccca
    19921 ggtgcccccg agcatccacc agaacgccct gcagcgcggc ttccgggtcg tcacggtgga
    19981 acgtccgggg accggaacct cgaccgacca ccgttaccgg cgcatcgcgg acttcgctcc
    20041 cgatgtcgag tcgctggtcg acgacctggg catcggccgc ttcgccgtgg tcggcctgtc
    20101 cggcggcggg ccgtacaccc tggcggtcgc gcggcatctg cccgagcggg tcgtcgtggc
    20161 gtcgctgctc ggtgggctcg gcccgacccg cggccccgac gcggtgtggt cctacacgcg
    20221 ggcgctgcgc ttcgtcgccc ccgcgttcga ggtgctgcgg accccgatcg gccgggtgct
    20281 cgcgtcgggg atcgacctcc tggcaccggt ggccgacccg gtgttctcgg cctacgccgc
    20341 cctgctcggc ttcgcggatc gtcccgtgct gcgtgacccg cagttccggg ccatgttcct
    20401 gcaggacctg ctcgcggcgc acgagctgcg ctcggtcgcc cacgacatgg cgctgttcag
    20461 ccgccactgg ggcttcgccc tcgacgaggt caccccgccg gtcatcgtct ggcaggggtt
    20521 ggccgacacg atcgtcccgc ccagccacgg gcaccaccag gcgtcccgcc tgccccgcgc
    20581 cgagctgcgg gtacgtccgg gcgaagggca cttcgccggc ttcgcggacg tggcgaccgt
    20641 gctcgatcgc ctgcgcgagg tgtgggccca ggagaccagc gaaccggtca ccgccccctg
    20701 accacgggtc agatccgggt gagggcgccg aggaccgcgg cggccttgga gcgggtctcc
    20761 tccagctcgg tccacggatc ggagccgtcg accacgccga cgccggcgaa cagccgggcc
    20821 gaggtcgggt cctcggcgat ctgcacgccg cggatgccga cgccgaacac gccgttgccc
    20881 tcggcgtcga cccagccgac cggtccggcg tagcgaccgc ggtcggcccg ttccagctcg
    20941 gcctgcaccg ccagctcggc gtcgtcgt
//
LOCUS       DAOQOD010000037        20952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_870_length_20952_cov_11.256113, whole genome shotgun sequence.
ACCESSION   DAOQOD010000037 DAOQOD010000000
VERSION     DAOQOD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20952
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_20952_cov_11.256113"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PLZ72_05620"
     CDS             complement(<1..1208)
                     /locus_tag="PLZ72_05620"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNA35810.1"
                     /translation="MTWTPPERTADAAAIHAAADADRRTNPELYSLDPDDVLTRVLER
                     TDGSTEAFLPGWRDGLEQFLGSLAEDGRLNALGARTMSEQATAKLVSAHRVARAIEAD
                     ATYADDLIPPIVIVGGWRTGTTFLFRLMARDPRLRAPLPVDLTRPWRVAGLDAAAREE
                     LLSRMDAAPSPLHVLNPDLRRVHDHGPRLPEECVLALGADLRSWGFSSTARLESYSRW
                     LAQQDLGPSYREYRRVLALLDDHDGRRFVLKSPAHLAEPGALVDTFPGAVVVNLHRDV
                     VETVASGASLFSVFRSTYSDEVDPFDVGRFQTDQTELWMRRAVEHRSAHDPATATFVD
                     LAYRDLVASPVEALRRVYRAAGMEPPDDLPGFVEAHHRASPRNEHGAHRYAPEDFGLD
                     PAELRERFADL"
     gene            complement(1205..2275)
                     /locus_tag="PLZ72_05625"
     CDS             complement(1205..2275)
                     /locus_tag="PLZ72_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HNA35811.1"
                     /translation="MTIEEDWAELTGSLAGAGARMSELTAGLGPVEAADGHRALLRAL
                     NNQLGRLEVDRERPELVPFNGWREKFFMDNPDFRYWVADVRHDRRYRISGTLGDAVFQ
                     SITAYATRGGPARSVGRIDSDSIGVDDDGRFTVVASSEPPESGDWLPLPEGADTIWVR
                     HFHADVDRDRLGDATIEPLDEPHEPPTLDPEALGRRIRRLARTMAAVPEVIAAATADE
                     RDRPNELRHWTEMAGGAAFTEPDITYVRGSWSLGPDEALVIEGDVVASRYWNVLLYSR
                     FLNSLDQRHRSVSRTSATATIVDGRYRFVVAPRDPGGAGDWLDTEGRPFGLVVMRWLQ
                     PQGELPLPRVHRCPIGDLERRP"
     gene            2350..3033
                     /gene="merB"
                     /locus_tag="PLZ72_05630"
     CDS             2350..3033
                     /gene="merB"
                     /locus_tag="PLZ72_05630"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HNA35812.1"
                     /translation="MTNLSSSTTLASMIDRTSDAFVAGCGFLHEPRHQRVGAAMYRIL
                     AGGQPASIADIASRAEVTTAEVEEWVGSWPAVFRDDDGAVTGFFGLSVDDGFNHRADV
                     DGMGTAWTWCTYDPLFIARVLGATVRVSSHCPVTGTEVRVTVTSDGVEAVEPAAAVMS
                     MMPPDLASFDDIITTLCHYIWFFADDEAARRWTAENPDTFVVSIEDGFEIGRRMTDAV
                     FDAVLAERT"
     gene            3084..4382
                     /locus_tag="PLZ72_05635"
     CDS             3084..4382
                     /locus_tag="PLZ72_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA35813.1"
                     /translation="MTADAATGTRPRRIVLVLPTETYRAADFIAAAAEVGVEVVVATE
                     HRPPLATEMESRLVVVDLDRPEEAAARIAAMGDRAPIDAVVGVDDQGVLTAAHAGEIL
                     GLAHNPPDAVAATRDKAQMRGVLASWGVRQPDFRVLVPGADVAEAAAAVGCPCVLKPL
                     SLSASTGVIRADTPADAVVVAERIRRILADHGRSPDEALLIERFVPGAEVSLEALLHD
                     GDLQVLALFDKPDPLDGPYFEETIYVTPSRLGGPEQTAVLAAAAEACRALGLRDGPVH
                     AELRVGPGADGEVAAWVLEVAARSIGGLCSRVLRFGTGISLEQLIVEHAVGRDVADSV
                     RTDGAAGVMMLPIPRSGVLTGVDGLDAARGVAGVTGVEITATIGRRIDALPDGGRYLG
                     FVFARGDTPDSVESALRRAHSELTVTIVDAPRSVDGRTPA"
     gene            4379..5812
                     /locus_tag="PLZ72_05640"
     CDS             4379..5812
                     /locus_tag="PLZ72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF040546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HNA35814.1"
                     /translation="MRVLLVSTYELGHQPLHVASPAAALERAGHRVSALDLSVEQWDP
                     QLIDDADAVAVSVPMHTAMRLALEAAGLVREQRPGLPVCLYGLYAGTSHDTVVGTLAD
                     RVISGEYEPALLEWVGSLDGHGSTDGADAAAGRNSSEQIVHLGRGRFDVPARHLLPPL
                     ERYARLSDGDEERLVGYVEASHGCTFSCRHCPVPTVYGGRIRIVDIDTILADIDQLVA
                     AGATHITFGDPDFLNGVHHSRRVVAALHERHPHLSFDCTTKVELILRHRDVWADFADA
                     GCRFVVSAFETVDDHVLEILDKGHTRAEMAEATAVLRSHGIEVRPSLLPFTPWTTLAS
                     LVELMDFVADHDLIGNVDPVHYSIRLLVPEDSLLVDHPAMGPHLGRYVADQLGYEWSS
                     ADPRTDELQVRVAAVVERAAAVDQPLATTFAEVDALVRAAAGMEPRHTPIDADVAARA
                     QSRPRLTEAWFCCAEPTEMQRRPLGER"
     gene            complement(5837..6448)
                     /locus_tag="PLZ72_05645"
     CDS             complement(5837..6448)
                     /locus_tag="PLZ72_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNA35815.1"
                     /translation="MKPVVLVTDAARAKVLELLAEEDDGTPMGLRIEITGENGPEYRY
                     DLSFDAVSAAEPGDSVVDWAGLTVIVPADSIEKLQGAQLDLPSGDGPGLVMRNPNRPD
                     PLAGMDVELTGDVTERINTLLEKVINPNLSQHGGFAELRAWEEPKAYILMGGGCQGCA
                     VSSITLRTGIERTLKDQIPEIEEVVDVTDHAAGTNPYYEPSKK"
     gene            complement(6516..6833)
                     /locus_tag="PLZ72_05650"
     CDS             complement(6516..6833)
                     /locus_tag="PLZ72_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNA35816.1"
                     /translation="MALTNDPIERIHQALRQVYDPELGLDIVSMGLIYRLEAEGDRIR
                     IDMTLTTPGCPVSETLPEMARQAALDALGPDGEDRLDFQVVWDPPWCPDLIDPESLAS
                     LGL"
     gene            complement(6934..8775)
                     /locus_tag="PLZ72_05655"
     CDS             complement(6934..8775)
                     /locus_tag="PLZ72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNA35817.1"
                     /translation="MDTTNPNKPRVAPVEPADGDHTGGNDVVIDVRPLIERGEEPFGT
                     IMEAVGTLDGRALVVVAPFEPVPLQGVLSAQGFHYASEQVGETEWRVRFEPGATSTAD
                     PSPATGPGPSGVAPPGPADTGTVTGTGTDTDAEAEVSPFTIRRPPSTTGTASGAAPTP
                     PTPGAAPAGPAAMMAMNPTANVPPAWLPLGFMAAAGVGLVGFGVAAATTAPTVVTFPR
                     SDEVIATVHLAVLAFLSTAVLGALHQFGPVVGARPLRSVPVGALTGVLFVPGAWAIPI
                     GFATGHVGVIQTGGVLATAAVVLAAWNLSRPLSAPDKGAPIVGLRMAVIYLVATAAFG
                     VTYAFDRSNFWFELLSHRVLAHAHLGLIGWLGLAYVSVAEKLWPMFLLAHRPHVRAGV
                     RAVWSVGLGAPVLTVGLLWPSELLSIVGGALVLAGLVSHLTSLAQVIHHRRRGLELLH
                     GYVLGAAACLVVAMVLGVVAGLAPVGVEVRTRLTAAEVVALILWLALAVLGHSHKIVP
                     FISWNRLRDRGIRTGRDGKPLLFAHLVDKRASQVTFGLALLGAAAALGGVLGSTTVIV
                     RGAGALLALAGLVAIANLVSGPLLMIRWHDRRPDQSDGSGRPAEVSS"
     gene            8904..9137
                     /locus_tag="PLZ72_05660"
     CDS             8904..9137
                     /locus_tag="PLZ72_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNA35818.1"
                     /translation="MPVTDPDLDVRNEIPKRRHELIFETYAALGAGEAFVLVNDHDPK
                     PLYYQFAAEHEGEFSWGYLEEGPDVWRVRIGRV"
     gene            complement(9864..10463)
                     /locus_tag="PLZ72_05665"
     CDS             complement(9864..10463)
                     /locus_tag="PLZ72_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35819.1"
                     /translation="MSRSTHLSPRRRTASCLLAVLAAALPLAAAACSDDSDASSSSTT
                     TTATADPGDSGHDHVEVGAVDFSFEDLPSTIDAGTQLTLRNDADRELHELVAFRLPDD
                     EKRPVAELLKLPESELMPMLGAPSTVLLAAPGEDQVAAVGDGTLTEPGRYLVMCSIPT
                     GVEPAEYLRAAAAANGQKPNVDGGPPHFTSGMFAELTVR"
     gene            complement(10543..11394)
                     /locus_tag="PLZ72_05670"
     CDS             complement(10543..11394)
                     /locus_tag="PLZ72_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA35820.1"
                     /translation="MDTTPTTHPPAPTERPRYRPVEIAPETWVIQDTAGEDQPGPAVH
                     MNSMLIRGAQPVVVDTGVPDNADRYFEDLFGLVDPADVRWVFVSHDDIDHYGNVGELM
                     RLCPDATLVTSWFLCERLGSELDVAPTRWRWLDDGETLDVGDRTLSAIRPPLYDSPTT
                     RGLFDPVTGVYWGSDCYACPVATGTEFVDDLDPDEWAGGTGVFARWNSPWVDLVDADR
                     YAAQCRRIEELPLRAIASTHGPTIGASDLARAHEIIRSAPTLATPPQPGQADLDAMLA
                     ALRPAAA"
     gene            11531..12685
                     /locus_tag="PLZ72_05675"
     CDS             11531..12685
                     /locus_tag="PLZ72_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA35821.1"
                     /translation="MAGSLTMDRVRSDVEVLSRAGLDVTTFVAEVEESVGRAVPHVAA
                     CMCTVDPATRLLTSTYKFGDLYGNDSRDLEWGLIEYSGTDPTAFSAMFAARRDSLGMV
                     IETADAVESSPRMEQLIVPHYDYADEVRLIARQGDLAWGGMALFRGEGDTPFSDAEIT
                     FLASLSEAYAVGLRVGLLTRCSGSLEECPPAPHADAGPAVAIFDAEDRVVQVSVGATQ
                     LLAEMTSRENSADASGTLAALVAGARRFQDGTSDVLPRGRVRLPSGRWLVLHASPLSS
                     VDGRRGDVVITMEEARPPEIVPLVVAAFGLTERERDVTRLVLQGSDTKEIAAVLHLSR
                     YTVQDHLKAVFDKADVRSRRELVSRVYFDQYAPRLGSEVAPSGWFAATGD"
     gene            12778..13212
                     /locus_tag="PLZ72_05680"
     CDS             12778..13212
                     /locus_tag="PLZ72_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35822.1"
                     /translation="MPSSSALAWIAFAHDAVDAAAAGSDDPAIIDPELAARLHELLEG
                     WAQHARQTEVLALSFEVPPDETEFLAHAFLRISDRWTSAADERGFDISPSEGDEFYNA
                     LVEAVIVALEHAEDDSGVEFGEALRSSWPRFDRLDPPPDEPE"
     gene            13315..14244
                     /locus_tag="PLZ72_05685"
     CDS             13315..14244
                     /locus_tag="PLZ72_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35823.1"
                     /translation="MRRQTFSMGIDPITRSTPERVELADGTTAWVVDTGPDDGLPVLF
                     VGGSATSATVVDLVSFLSSAREELGIRLISVERNGFGDTPFDPDGDYDDFARTAIGVL
                     DAKGVDDFSIVAISGGGPYAQRVAASVPDRLRSVHFAAAYTGTPDAGPPKDLCRLEPQ
                     SRAALAQGYSDDPASWWAFGDDAIAHRIPGFLDAAVADAERALRDPRAVAHEFDLFCH
                     PTDVDLSVVDAPAFLYYSPQDTSTPLPFADWYAQRLPHVVADRRDLTGDHDEQYRHWD
                     QILVDLAWPDDPPTLVCGEVPAGSTSLPGLCMD"
     gene            14309..16060
                     /locus_tag="PLZ72_05690"
     CDS             14309..16060
                     /locus_tag="PLZ72_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA35824.1"
                     /translation="MADAHDLIVIGGNAGGLSAAVFARRSGLDRVTVLEPGTTLALPG
                     VAGEFGLDVSYGETVVSVELQDDDLVVTTHRQCYRSRAVLVALRSPIEDWAPDIPTVT
                     GERVHIDEIPSPANDVDVLVVGHTDQAVELTVRLADLGAGVVLAAGGMNPRRLSPVGA
                     QLLNDLERERRATLLYSSRPQQIADVDGFPMAYFNDRRTPDLQFDHVVFSSGRRAVGP
                     ADVGASDAALGTGRLWFLGQDDETPEGAPSAPGASIGRDLVTAVFPDAAIPAPEPSAW
                     SRHEVPGVTEELRQEHYNATITHFEPTHSDLWVLRVRPDHGSVSHLPGQYASLGLGYW
                     EERVDDAIEANIDERWDKLVRRSYSISHRIFDEHGYLADEVGSDELEFYIVLVPPTPD
                     NVPGLTPRLALKKPGDRIYLGPKVAGRYTLAPVTDPADTVVFLSTGTGEAPQNAMVTE
                     LLRKGHHGPIVSAVSVRQWADLGYLDQHRRLEQRFANYHYVPLPTREPDVPKRYIQDL
                     IAGDVFAADLGITLDPQNTHVFLCGNPAMIGLPEGGDDGDQTFPETVGVVQLLVERGF
                     TLDHRKKRGNIHYEEYW"
     gene            16116..17165
                     /locus_tag="PLZ72_05695"
     CDS             16116..17165
                     /locus_tag="PLZ72_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA35825.1"
                     /translation="MKVSVSVGSAYYDGSRWEDLVAYVAAAERLGASTVWSAEAWGMD
                     AVASLGYLAAVTDRIRLGSGIMQISARTPAATAMTALSLAQLSGDRFSLGLGVSGPQV
                     VEGLHGAEFADPVGRLREYIEIVRMGLRGDRLAYEGRHYVLPRPGGEGKALRSSVPPR
                     PDLPIHLATLGPRSLELTGELADGWLGTSFIPEQASVMLDPIRRGAERTGRAFSDIEI
                     EVNAHLAVSDDVESLIGIAKFGMAFSLGAMGSATTNFYNNAYSRAGFADVADRVRRLW
                     VDGDKDAAIAAVPDEMVLSAYLIGTESMVRDRIRAYRDAGVTTLRLGPSGSTAAEQIE
                     HLEMAVDLIGSAAAD"
     gene            17168..18709
                     /locus_tag="PLZ72_05700"
     CDS             17168..18709
                     /locus_tag="PLZ72_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA35826.1"
                     /translation="MAHPGHHAADRPDDTAVVVLGDGAEPVESATWAELRARAVAVAD
                     DLWGLGLRAGDPVAFCLENRVDFFALVWACQDAGFRYTPVPTRLTAEEVRHILSDCGA
                     RVVLHSARTRSVVVPACSELDGVIRIDLDPGDVFGAGSGEAPRYERVEGVALLYSSGT
                     TGRPKGVWRAAPPEPIEELPPGDQLLAAAYGVGPGSVYLSTAPLYHSAPLTFLVQMGR
                     IGATTVVMDRFDPGAALAAIERYRVTHSQWVPTMFVRLLRLDEAERRAHDLSSHTFAI
                     HGAGPCPVPVKEAMLDWWGPIIHEYYAGTEGAGTCVIGPQEWLARKGSVGRPIRGRVM
                     ILDDDGTELPVGQDGQVWFSESTDFRYLHDDAKTAGVRRADAATFGDLGHLDDDGYLY
                     LTGRQAFTINVGGVNVYPREIELVLLEHPAVADVAVFGRPDDEYGEQVVAVVEVAAGI
                     EPDTVLEADTVLEADTVLEAELLSHCRDRLATFKLPRTVHLVDELPREPTGKLRVADL
                     QARYR"
     gene            complement(18722..19165)
                     /locus_tag="PLZ72_05705"
     CDS             complement(18722..19165)
                     /locus_tag="PLZ72_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNA35827.1"
                     /translation="MSAFDAPYEPSPWEPISEEVARYESSAGTEPSALVGESWMILWT
                     IGARSGSVRKTPLVRIADGAGRYAVIGSQGGAPTNPNWVHNLRANPVARIQDGADLHD
                     LTAREVDGDEKAQWWARGQAIWPDYDTYQASTDRTIPVFVLEPAG"
     gene            19285..19605
                     /locus_tag="PLZ72_05710"
     CDS             19285..19605
                     /locus_tag="PLZ72_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35828.1"
                     /translation="MGPDQAPGSSDWMVPIGILAGLLGTAAAGVGALIALLSTNSCGA
                     FADSCDTYGQPAEEFLPAVGVMALGAAAAVVGFVLLVVGMSRAARARQSSDPRSEPGP
                     PLLR"
     gene            complement(19610..20674)
                     /locus_tag="PLZ72_05715"
     CDS             complement(19610..20674)
                     /locus_tag="PLZ72_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35829.1"
                     /translation="MAGQFMKRSGSTARRRPRRLTAAALALAAAALLVTACAPAPEPG
                     QRYRVPADFAEGLLTALGDIHGPPPGANVDGCVPSTAHPRPVILLHGFAGNMADNMGG
                     ISPFLANQGYCVFALTYGDPDGSLFGGLHDIRRSSMEEFGPFVDDVLARTGARQVDVV
                     GHSEGSIMPRWWMRFGPSVHSDGTPKVATFVGVGPATHGADLGGNAATLRSIPFFAGV
                     IDTMVQNGCGACEQVLSGSSFLKQLNSSEPQPGERFTGPTQPGVRYMMLATQLDNFLV
                     PYQYGFIDHPAVTNTTVQDVCAIDEADHLSIVFDPVAYDVIDNFLDPATATTPRCVAT
                     QPVFAPIDQRDWPPPTLPAD"
     gene            complement(20765..>20952)
                     /locus_tag="PLZ72_05720"
     CDS             complement(20765..>20952)
                     /locus_tag="PLZ72_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35830.1"
                     /translation="MLHWGGWGGSFIIMDLDRRTTFSYTMNKMAAGIVGSPTGEAYLR
                     AGYAALGVDVPGHVVAL"
BASE COUNT         2970 a         7367 c         7653 g         2962 t
ORIGIN      
        1 aggtcggcga agcgctcccg gagctcggcg ggatccagcc cgaagtcctc gggcgcgtac
       61 cggtgggcgc cgtgctcgtt gcgcggcgac gcccggtggt gcgcctcgac gaaccccggg
      121 aggtcgtcgg gcggttccat ccccgcggcc cggtagacgc ggcgcagcgc ctcgaccggc
      181 gacgcgacca ggtcccggta ggccaggtcc acgaacgtcg cggtcgccgg gtcgtgtgcg
      241 gagcggtgct cgacggcgcg ccgcatccac agctcggtct ggtcggtctg gaacctgccg
      301 acgtcgaacg ggtcgacctc gtcgctgtag gtcgagcgga acaccgagaa cagcgacgcc
      361 cccgatgcga cggtctcgac gacgtcccgg tgcaggttga cgaccaccgc cccggggaac
      421 gtgtcgacca gggcgcccgg ctcggcgagg tgggccggtg acttcagcac gaagcgtcgt
      481 ccgtcgtggt cgtcgagcag ggcgagcacc cggcggtact cccggtacga cgggccgagg
      541 tcctgctggg ccagccaccg cgagtagctc tcgaggcggg ccgtcgacga gaagccccag
      601 ctgcgcaggt cggctccgag ggcgagcacg cactcctcgg gcagacgggg tccgtggtcg
      661 tgcacccggc gcaggtcggg gttgagcacg tgcagcggag aaggagccgc gtccatgcgg
      721 ctcaacagct cctcccgcgc cgcggcgtcg agcccggcga cccgccacgg ccgggtgaga
      781 tccaccggca gcggtgcccg caacctcggg tcgcgggcca tcagccggaa caggaacgtg
      841 gtcccggtgc gccagccgcc gacgatcacg atcggtggga tcaggtcgtc ggcgtaggtg
      901 gcgtcggcct cgatcgcccg ggccacccgg tgggcagaga ccagcttggc cgtcgcctgc
      961 tcggacatcg tgcgggcgcc cagcgcgttg agccggccgt cttcggccag cgaccccagg
     1021 aactgctcga ggccgtcgcg ccagccgggc aggaaggcct cggtggagcc gtcggtgcgt
     1081 tcgagcaccc gcgtcaggac gtcgtcgggg tcgagcgagt agagctctgg gttcgtccga
     1141 cggtccgcgt cggccgccgc gtggatggcg gctgcgtccg cggtccgctc gggtggggtc
     1201 caggtcacgg ccgccgctcc aggtcaccga tcgggcagcg gtgcacgcgg gggagcggga
     1261 gctcgccctg cggttgcagc cagcgcatca cgaccaggcc gaacggacgg ccctcggtgt
     1321 cgagccagtc gccggctccg ccgggatcgc ggggcgcgac cacgaaccgg tagcggccgt
     1381 cgacgatcgt cgcggtggcc gacgtgcgcg acaccgagcg gtgccgctgg tcgagcgagt
     1441 tcaggaaccg gctgtagagc agcacgttcc agtaccggct ggccacgacg tcgccctcga
     1501 tcaccagcgc ctcgtcgggt ccgaggctcc aggacccacg gacgtaggtg atgtccggtt
     1561 cggtgaacgc ggcccctccg gccatctcgg tccagtggcg gagctcgttc ggccgatccc
     1621 gctcgtcggc tgtggccgcg gcgatcacct cgggaacggc ggccatcgtg cgggccaggc
     1681 gtcggatgcg gcgccccagc gcctcgggat cgagcgtcgg cggctcgtgc ggttcgtcga
     1741 gcggctcgat cgtggcgtcc ccgaggcggt cccgatcgac atcggcgtgg aagtggcgga
     1801 cccagatcgt gtcggcgccc tcgggtagag ggagccagtc cccggactcc ggcggctcag
     1861 acgacgcgac caccgtgaaa cgcccgtcgt cgtcgacgcc gatggagtcg gagtcgatcc
     1921 gcccgaccga gcgggcgggt ccgccccggg tcgcgtacgc ggtgatcgac tggaacacgg
     1981 cgtcgccgag cgtgccggag atccggtagc ggcggtcgtg gcgcacgtcg gcgacccagt
     2041 agcggaagtc ggggttgtcc atgaagaact tctcgcgcca cccgttgaac ggcaccagct
     2101 cgggccgctc ccgatcgacc tccagacgac cgagctggtt gttcagcgcc cgcagcagcg
     2161 ctcggtgccc gtcggcggcc tcgacagggc cgagcccggc ggtcagctcc gacatgcggg
     2221 ctccggctcc ggccaggctc ccggtcagtt ccgcccagtc ctcctcgatc gtcacccggt
     2281 caccttgtca cgatcggcgt gccggtcggg gcagcgggcg gagccggtcc tgtcccctac
     2341 gatccgtgga tgaccaacct gtcgtcgtcg accacgctcg cctcgatgat cgaccgcacc
     2401 agcgacgcgt tcgtcgccgg ctgcgggttc ctccacgagc cgcgacacca gcgggtcggc
     2461 gcggccatgt accggatcct ggccggtgga caaccggcgt cgatcgccga catcgcctcg
     2521 agggccgagg tcacgaccgc cgaggtcgag gagtgggtcg ggtcctggcc ggcggtgttc
     2581 cgcgacgacg acggagcggt gacagggttc ttcggcctca gcgtcgacga cggcttcaac
     2641 caccgggccg acgtcgacgg catgggtacg gcgtggacct ggtgcaccta cgacccgctg
     2701 ttcatcgccc gggtgctcgg cgccaccgtc cgggtcagct cgcactgccc ggtcaccggg
     2761 accgaggtcc gcgtcaccgt cacctccgac ggggtcgagg cggtcgagcc ggccgccgcg
     2821 gtgatgtcga tgatgccgcc ggacctggcg tcgttcgacg acatcatcac cacgctgtgc
     2881 cactacatct ggttcttcgc ggacgacgag gcggcgcgcc ggtggaccgc ggagaacccc
     2941 gacacgttcg tggtgtcgat cgaggacggc ttcgagatcg gtcgccggat gaccgacgcc
     3001 gtgttcgacg ccgtcctcgc cgagcggacc tgagcagccg gcaccggaga tccgctcgga
     3061 cgggcccgca gtagcctgcg gacgtgacgg cggacgcggc gaccgggacc cgaccccggc
     3121 gcatcgtgct ggtgctgccc accgagacct accgggccgc cgacttcatc gcggcggccg
     3181 ccgaggtggg ggtggaggtg gtcgtcgcca ccgagcaccg accgcccctc gccacagaga
     3241 tggagagccg gctggtggtc gtcgacctcg accgacccga ggaggcagcg gcgcggatcg
     3301 ccgcgatggg ggaccgggcc ccgatcgacg cggtggtcgg cgtcgacgac cagggagtgc
     3361 tcaccgccgc gcacgccggt gagatcctcg gcctggccca caacccgccc gacgcggtcg
     3421 ccgccacccg cgacaaggcc cagatgcgcg gggtgctcgc gtcgtgggga gttcgtcagc
     3481 ccgacttccg cgtcctggtc cccggagcgg acgtcgccga ggccgcagcc gcggtcggct
     3541 gtccgtgcgt cctcaagccg ctgtcgctgt cggcgagcac cggcgtcatc cgggccgaca
     3601 cccccgcgga cgcggtcgtg gtcgccgagc ggatccgacg gatcctggcc gaccacgggc
     3661 ggtcgccgga cgaggcgttg ctcatcgagc ggttcgtgcc cggtgcggag gtgtcgctcg
     3721 aagcgctcct gcacgacggt gacctgcagg tgctggccct gttcgacaag ccggacccgc
     3781 tcgacggccc ctacttcgag gagacgatct acgtcacgcc gtcgcggttg ggcgggccgg
     3841 agcagaccgc ggtgctggcc gccgcggccg aggcctgtcg ggcgctcggc ctgcgcgacg
     3901 gaccggtgca cgccgagctg cgggtcgggc cgggagcgga cggcgaagtc gccgcctggg
     3961 tgctcgaggt cgcggcccgc tcgatcggcg ggctgtgctc ccgggtgctg aggttcggga
     4021 ccgggatctc gctcgaacag ctcatcgtcg agcacgccgt gggtcgggac gtcgccgact
     4081 ccgtacggac cgacggcgct gccggggtga tgatgctgcc gatcccgcga tcgggcgtgc
     4141 tgaccggcgt cgacggcctg gacgccgccc gcggcgtggc gggcgtgacc ggcgtcgaga
     4201 tcaccgcgac gatcggccgc cgcatcgacg cgctgcccga cggcgggcgt tacctcggct
     4261 tcgtgttcgc ccggggcgac acgcccgact cggtggagtc ggctctgcgc cgggcccact
     4321 cggagctcac cgtcaccatc gtcgacgcac cgcgctctgt cgacggcagg actccggcat
     4381 gagggtcctg ctcgtgtcca cctacgagct gggtcatcag ccgctgcacg tcgcgtcccc
     4441 tgccgccgcg ctggaacgcg ccggccaccg ggtcagcgcg ctcgacctgt cggtcgagca
     4501 gtgggacccg cagctgatcg acgacgccga cgccgtcgcg gtgtcggttc cgatgcacac
     4561 cgcgatgcgg ctggcgttgg aggccgccgg cctggtgcgt gagcagcggc ccggcctgcc
     4621 ggtgtgcctc tacgggctgt acgccgggac cagccacgac acggtggtcg gcacgctggc
     4681 ggaccgcgtg atctccggcg agtacgagcc cgccctcctg gagtgggtcg gttcgctgga
     4741 cgggcacggt tccaccgacg gggccgacgc ggcggccggc cgtaacagca gcgaacagat
     4801 cgtgcacctc gggcggggcc gcttcgacgt ccccgcccgg cacctgctgc cgccgttgga
     4861 gcgctacgcc cggctgtccg acggtgacga ggagcgcctg gtcggctacg tcgaggccag
     4921 ccacggctgc acgttcagct gccggcactg cccggtgccg accgtgtacg gcggccggat
     4981 ccgcatcgtc gacatcgaca cgatcctggc cgacatcgac cagctggtcg cggcgggggc
     5041 cacccacatc accttcggcg accccgactt cctcaacggc gtgcaccact cccggcgggt
     5101 cgtcgccgcg ctgcacgaac ggcacccgca tctcagcttc gactgcacga ccaaggtcga
     5161 gctgatcctc cgccaccgcg acgtgtgggc cgacttcgcc gacgccgggt gccggttcgt
     5221 cgtctcggcg ttcgagaccg tcgacgacca cgtgctcgag atcctcgaca agggccacac
     5281 ccgggccgag atggcggagg ccaccgccgt cctgcgcagc cacggcatcg aggtgcggcc
     5341 gtccctgttg cccttcaccc cttggaccac cctcgccagc ctggtcgagc tgatggactt
     5401 cgttgccgac cacgacctga tcggaaacgt cgacccggtg cactacagca tccggctcct
     5461 ggtgcccgag gactcgttgc tggtcgacca cccggccatg ggaccgcacc tcggtcgcta
     5521 cgtcgccgac cagctcggct acgagtggag ctcggccgac ccccggaccg acgagttgca
     5581 ggtgcgtgtc gccgccgtgg tcgaacgcgc cgccgccgtc gaccagccgc tggcgacgac
     5641 gttcgccgag gtcgacgccc tggtgcgggc cgcggccggg atggagccga ggcacacccc
     5701 gatcgacgcc gacgtggcgg cgcgggccca gagccgaccg cggctcaccg aggcgtggtt
     5761 ctgctgcgcc gagccgaccg agatgcagcg ccggccgctc ggtgagcgct gagcggtccg
     5821 ggtcgccggg cggggactac ttcttggacg gctcgtagta cgggttggtg cccgcggcgt
     5881 ggtcggtgac gtcgacgacc tcttcgatct cgggaatctg gtccttgagc gtgcgttcga
     5941 tcccggtgcg cagcgtgatc gacgacaccg cgcacccctg gcacccgccg cccatcagga
     6001 tgtaggcctt gggctcctcc caggccctca gctcggcgaa gccgccgtgt tgggacaggt
     6061 tcgggttgat caccttctcg agcagggtgt tgatgcgctc ggtgacgtcg ccggtcagct
     6121 ccacgtccat gcccgccaac ggatcgggcc ggttcgggtt gcgcatgacc aggccggggc
     6181 cgtcgccgga gggcaggtcg agctgtgcgc cctggagctt ctcgatcgag tccgcgggca
     6241 cgatcaccgt gagccccgcc cagtcgacga ccgagtcgcc gggttcggcc gcggacacgg
     6301 cgtcgaagga caggtcgtag cggtactcgg gtccgttctc gccggtgatc tcgatccgca
     6361 ggcccatcgg ggtcccgtcg tcctcctcgg cgagcagctc gaggaccttg gcccgggccg
     6421 cgtcggtcac gagcaccacc ggcttcaccg agtcaccccg gatcgggtcg tggtcgtggc
     6481 cgcaggcgag ggggcggggg gtcggtcggt ggggatcaca ggcccaagct tgccaggctc
     6541 tcggggtcga tcagatcggg acaccacggc gggtcccaca ccacctggaa gtcgagccgg
     6601 tcctcaccgt ccggtccgag ggcgtcgagc gcggcctggc gtgccatctc gggaagggtc
     6661 tccgacaccg ggcagcccgg tgtggtcagg gtcatgtcga tcctgatgcg gtcgccttcg
     6721 gcctcgaggc ggtagatgag ccccatgctg acgatgtcga gcccgagctc ggggtcgtac
     6781 acctggcgga gggcttggtg gatccgttcg atcgggtcgt tggtcagagc catggcgggt
     6841 tcctcggtga tgaaggcgca gcggagtcgt gtcgaacggt ccgcggtcgg ttccgctccg
     6901 gccaggctac ggcggtcgct cccggaggtg agctcacgac gacacctcgg ccggtcggcc
     6961 cgaaccgtcg gactgatccg gccgccggtc gtgccagcgg atcatgagca gcggacccga
     7021 caccaggttg gcgatcgcga cgaggccggc gagcgccagc agggcgccgg cgccgcggac
     7081 gatgacggtg gtcgagccga gcacgccgcc gagcgcggcc gccgcgccca gcagcgccag
     7141 accgaaggtg acctgggacg cgcgcttgtc gaccaggtgg gcgaagagca gcggctttcc
     7201 gtcgcggccg gtcctgatgc cgcggtcccg caggcggttc cacgagatga aggggacgat
     7261 cttgtgggag tggccgagca cggccaaggc caaccacagg atcagcgcga ccacttcggc
     7321 cgcggtcagc cgggtccgga cctcgacgcc gaccggcgcc agccccgcga ccaccccgag
     7381 caccatggcg accaccaggc acgccgccgc gccgagcacg tatccgtgga ggagctcgag
     7441 gccccgccgc cgatggtgga tcacctgggc gagcgaggtg aggtgcgaca cgagcccggc
     7501 cagcaccagc gccccaccga cgatcgacag gagctccgag ggccacagca acccgacggt
     7561 gaggaccggg gctccgaggc cgaccgacca cacggcgcgc acgccggccc gcacatgggg
     7621 tcggtgcgcc aggaggaaca tgggccagag cttctcggcc accgacacgt aggccaggcc
     7681 gagccatccg atcagtccga ggtgggcgtg cgccaggacc cggtgcgaca ggagctcgaa
     7741 ccagaagttg ctgcggtcga acgcgtaggt gacaccgaac gcagccgtgg cgaccaggta
     7801 gatcaccgcc atgcgcagcc cgacgatcgg cgctcccttg tccggggcgg agagcggacg
     7861 cgagaggttc cacgcggcca gaaccactgc cgcggtcgcc aggacgcccc cggtctggat
     7921 caccccgacg tggccggtgg cgaacccgat cgggatcgcc cacgccccgg ggacgaacag
     7981 cacgccggtc aacgctccga ccggcaccga gcgcagcggg cgggcaccga cgaccggtcc
     8041 gaactggtgc agggcgccga ggaccgcggt ggagaggaag gcgagcacgg cgaggtgcac
     8101 ggtggcgatg acctcgtcgc tgcgggggaa ggtgaccacg gtcggggcgg tggtcgcggc
     8161 ggccacgccg aagccgacca ggcccactcc ggccgcggcc atgaacccga gcggcaacca
     8221 cgcgggaggc acgttggcgg tggggttcat cgccatcatc gcggccggcc cggcgggcgc
     8281 cgcgccaggg gtgggcggcg tgggtgcggc gcccgacgcg gtgcccgtgg tcgacggtgg
     8341 gcgacggatg gtgaacggcg acacctccgc ctccgcgtcc gtgtcggtgc cggtgccggt
     8401 gacggtgccg gtgtcggccg ggccgggtgg tgcgacaccg ctcggaccgg gtccggtggc
     8461 gggggacgga tcggccgtac tggtggctcc gggctcgaag cggacccgcc actcggtctc
     8521 gccgacctgc tcggaggcgt agtggaagcc ctgggcggac agcacgccct gcaacgggac
     8581 cggctcgaag ggtgcgacga cgaccagggc tcgtccgtcg agcgttccca ccgcctccat
     8641 gatcgttccg aacggctcct cgccgcgttc gatgagcggt cggacgtcga tgacgacgtc
     8701 gtttcctccg gtgtgatcac cgtcggcggg ctcgaccgga gcgacgcgtg gcttgttcgg
     8761 gttcgttgtg tccatccgga tcctccgacg accgaccctg gcggaataac tctatcgaga
     8821 ggtagaaaaa tagggaaggc cggtccgagc agaccggcac caccatcggt gcccgcgggc
     8881 accacgatca gaggagcgct cccatgccgg tcaccgatcc cgatctcgac gtccgcaacg
     8941 agatcccgaa gcgtcgccac gagctcatct tcgagaccta cgcggcgctc ggtgcgggcg
     9001 aggccttcgt gctggtcaac gaccacgatc ccaagccgct ctactaccag ttcgcggccg
     9061 agcacgaggg tgagttctcc tggggctacc tcgaggaggg cccggacgtc tggcgggtcc
     9121 gcatcgggcg ggtctgagcc gacacggcac ccgatcgcgg cccgacgcga gaggagagcg
     9181 agacgatcgc gcccagcgac gcgagggcga gcatcgtggc ggtcacccgc agcgagctcc
     9241 ggtgacggtg gacgaggcgg tggccgatca tccacgggtg gtggaagccc ggccggcgca
     9301 tcacccgggt cgtgatcgtc ccgagagccc gagcgctccg acgggagacg cggaggtggc
     9361 gcccgcgtcg ctgcaccgtg accgccacgg agatgccgga gatccgggcg aacctcggcg
     9421 atgacgcgag gggttcgatc gcacggaggt agcgcgcctc gtcgatcagg atcgctcggg
     9481 cccacccgat ggcgacggcg accacgcccg ccgatccgac cactatcagt gacatcgcga
     9541 ccacactagt tggtcgtcga tcgggcgaac aggtccggac cggcgacgtc ggaccggttg
     9601 cccgtgacag tctgtcgggc gtcgaggtct ccgaggagcg gaccgaaggg gggtgtgatg
     9661 gccaagaaga agggcaagaa gaaggacaag ggcaagaaga aggacaagaa gggcaagaag
     9721 aaggacaagg gcaagaaggg caagaagaag tagcgccgga tcggaggcgg ggcgcacgct
     9781 ccgtcgacgg ccctgaccgg acggccgacg gccccgaggg atgcaccctc ggggccgtcg
     9841 tcgcccgtgc gtcgatcggg ggttcagcgc acggtgagct cggcgaacat gccggaggtg
     9901 aagtgcggcg gtccgccgtc gacgttcggc ttctggccgt tggccgcggc tgcagcccgg
     9961 aggtactcgg ccggctcgac gccggtgggg atcgagcaca tcacgaggta ccggccgggc
    10021 tcggtcaggg tgccgtcccc gaccgccgcg acctggtcct cgcccggggc ggcgagcagg
    10081 acggtgctcg gcgcgcccag catcggcatg agctcggact cgggcagctt cagcagctcg
    10141 gccacggggc gcttctcgtc gtcggggaga cggaacgcga ccagctcgtg cagctcgcgg
    10201 tcggcgtcgt tgcgcagcgt cagctgcgtg ccggcgtcga tggtcgaggg caggtcctcg
    10261 aacgagaagt cgaccgcgcc gacctccacg tggtcgtggc cgctgtcgcc agggtcggcc
    10321 gtggcggtgg tcgtcgtgct cgacgacgag gcgtcgctgt cgtcgctgca cgcggcggcc
    10381 gccaacggga gggcggccgc gagcacggcc agcaggcacg aggcggtccg gcgtcgcggt
    10441 gagaggtggg tggatcggga catgggggtt cctttcgggg tggatgtctg ttcaggggat
    10501 gggactgatc ggttgagggg gtcgggcggt agagcccggc gatcacgccg ccgcggggcg
    10561 gagggcggcc aacatcgcgt cgaggtcggc ctggccgggc tggggcgggg tcgccagcgt
    10621 cggtgcggac cggatgatct cgtgggcccg ggccaggtcg ctggccccga tcgtcgggcc
    10681 gtgcgtgctg gcgatggccc gcaacggcag ctcctcgatc cgacggcact gcgcggcgta
    10741 gcggtcggcg tcgacgagat cgacccacgg gctgttccag cgggcgaaca cgccggtgcc
    10801 gcccgcccac tcgtcggggt cgaggtcgtc gacgaactcg gtcccggtcg cgaccggaca
    10861 cgcgtagcag tccgagcccc agtagacgcc ggtgacggga tcgaacaggc cccgggtggt
    10921 gggcgagtcg tacaggggag gacggatcgc gctcagggtg cggtcgccga cgtccagcgt
    10981 ctcgccgtcg tcgagccacc gccaacgtgt cggagcgacg tcgagctcgg agccgagccg
    11041 ctcgcacagg aaccagctgg tgacgagcgt cgcgtccggg cagagccgca tcagctcgcc
    11101 gacgttgccg tagtggtcga tgtcgtcgtg gctgacgaac acccagcgga cgtccgccgg
    11161 gtcgacgagg ccgaacaggt cctcgaagta gcggtcggcg ttgtcgggaa ccccggtgtc
    11221 gaccacgacg ggctgggcgc ctcggatcag catcgagttc atgtggaccg ccggtccggg
    11281 ctggtcctcg ccggcggtgt cctggatgac ccaggtctcc ggggcgatct cgacgggccg
    11341 gtagcggggt cgctccgtcg gagcgggggg atgggtggtg ggtgtggtgt ccatgtcggc
    11401 cacgttgagg cacgggcctc gggctcgaac atcccgtagc ggcggggact tccctcccgc
    11461 cgggtgcgga caacccaccc cccagaggtg tgggatggca cgacgggcgc gtccgtccga
    11521 tactgctcac atggccggaa gcctgaccat ggacagggtg cgaagcgacg tcgaggtcct
    11581 ctcgcgggcc ggactggacg tgacgacctt cgtcgccgag gtggaggagt cggtcggtcg
    11641 ggccgttccc cacgtcgccg cgtgcatgtg caccgtcgat ccggccaccc gactcctcac
    11701 cagcacctac aagttcggcg acctgtacgg gaacgactcc cgcgacctcg agtgggggct
    11761 catcgagtac agcgggaccg acccgacggc gttctcggcg atgttcgcgg cgcgccggga
    11821 ctcgctcggg atggtgatcg agaccgccga cgcggtcgag tcgagcccga ggatggagca
    11881 gctgatcgtc ccgcactacg actacgcgga cgaggtccgg ctgatcgccc gacagggcga
    11941 cctggcctgg ggtggcatgg cgctgttccg gggggagggc gacacgccgt tctccgacgc
    12001 cgagatcacg ttcctcgcct cgctgtcgga ggcctacgcc gtcggactgc gggtcgggct
    12061 gctcacccgt tgcagcggtt ccctcgagga gtgcccgccc gcgccgcacg cggatgccgg
    12121 gccggcggtg gcgatcttcg acgccgagga tcgggtggtt caggtgtcgg tcggagccac
    12181 gcagctactg gccgagatga ccagcaggga gaactcggcc gacgcgtcag ggaccctggc
    12241 ggcactggtc gccggcgcac gtcggttcca ggacggcacc tccgacgttc tgccccgcgg
    12301 tcgcgtgcga ctgccgtcgg ggcggtggct ggtcctgcac gcgtcgccgc tgagctcggt
    12361 cgacggtcgt cgcggcgacg tggtcatcac catggaggaa gcccgcccgc ccgagatcgt
    12421 gccgctggtc gtcgcggcgt tcgggttgac cgaacgcgaa cgtgacgtga cccggctcgt
    12481 cctgcaggga tcggacacca aggagatcgc cgcggttctg cacctgtctc gctacacggt
    12541 gcaggaccac ctcaaggccg tgttcgacaa ggccgacgtg cgcagccggc gtgagctggt
    12601 ctcgcgggtc tacttcgacc agtacgcacc ccggctgggg tcggaggtcg ctccgagcgg
    12661 ttggttcgcc gccaccggcg actgacaccc ccgctcgatc gtcggagcga tcccgattcc
    12721 gggaccggcg ctcgaccgcg gcgataccct gacggcgtgc acgtgacggt ctctcccgtg
    12781 ccttcctcca gcgccctcgc ctggatcgcc ttcgcccacg acgcggtgga cgccgccgcg
    12841 gcgggatccg acgatccggc catcatcgac cccgagctgg cggcccgact gcacgagctg
    12901 ctggagggct gggcgcagca cgcccggcag acagaggtcc tggccctgtc gttcgaggtc
    12961 ccacccgatg agaccgagtt cctcgcccac gccttcctgc gcatctcgga ccggtggacc
    13021 tccgccgcgg acgagcgcgg cttcgacatc tcaccgagcg agggcgacga gttctacaac
    13081 gccctggtcg aggccgtgat cgtcgccctc gagcacgccg aggacgactc gggtgtcgag
    13141 ttcggcgagg cgctccggtc gagttggccc cgattcgatc ggctggaccc gcccccggac
    13201 gaacccgagt agggggcccg gcgtgtccct ccggacagct cgccgctggc tcgagcctcc
    13261 ggtgcgtcgg tgcgtcggtt ccccattcga cgggacgggg tgccaggatg cgccatgcga
    13321 cggcagacct tctcgatggg gatcgacccg atcacccgct cgactcccga acgggtcgag
    13381 ctcgcggacg ggaccaccgc ctgggtcgtc gacacgggac ccgacgacgg cctccccgtc
    13441 ctgttcgtcg gaggctcggc gaccagcgcg acggtcgtcg acctggtcag cttcctgtcc
    13501 tcggcgcgcg aggagctcgg gatccggctg atctcggtgg agcgcaacgg cttcggcgac
    13561 accccgttcg accccgacgg cgactacgac gacttcgccc gcaccgcgat cggggtcctg
    13621 gacgccaagg gagtcgacga cttctcgatc gtcgcgatct ccgggggcgg cccctacgcc
    13681 cagcgggtcg cggcgtcggt gccggaccgt ctgcgatccg tgcacttcgc cgcggcgtac
    13741 accgggacac ccgacgccgg cccgccgaag gacctgtgca ggctcgagcc gcagtcccgc
    13801 gccgcgctcg ctcagggcta cagcgacgac cccgcgtcct ggtgggcctt cggtgacgac
    13861 gccatcgccc accgcatccc cggcttcctc gacgccgccg tggctgacgc ggaacgtgcc
    13921 ctccgcgacc ccagggcggt ggcccacgag ttcgacttgt tctgccaccc gaccgacgtc
    13981 gacctctcgg tggtcgacgc gccggcgttc ctctactaca gcccgcagga cacctcgacg
    14041 ccgctgccgt tcgcggactg gtacgcgcag cggctcccgc acgtggtcgc cgaccgtcgg
    14101 gacctgaccg gcgaccacga cgagcagtac cggcactggg accagatcct ggtcgacctg
    14161 gcatggccgg acgatccccc gaccctcgtc tgcggggagg tccccgccgg gtcgacgtcg
    14221 ctgccgggcc tctgcatgga ctgatcgtcg cgagccgatg ctctcccggc tttggagcag
    14281 ggggtgggcc gcccgttagg ttgccccgat ggctgacgca cacgacctca tcgtcatcgg
    14341 cggcaacgcc ggcggcctct cggccgcggt gttcgcccgt cggagcggac tcgaccgcgt
    14401 caccgtgctc gaacccggaa ccaccctcgc cctgcccggc gtggccggcg agttcggact
    14461 cgacgtctcc tacggtgaga cggtcgtgtc ggtcgagctc caggacgacg acctggtcgt
    14521 caccacccac cgccagtgct accgcagccg ggccgtcctg gtggcactgc gctcacccat
    14581 cgaggactgg gcgcccgaca tcccgaccgt caccggcgaa cgggtccaca tcgacgagat
    14641 cccctcgccc gccaacgacg tcgacgtcct ggtcgtcggg cacaccgacc aggcggtcga
    14701 gctgaccgtg cggctggccg acctcggcgc cggcgtcgtg ctggccgcag gcgggatgaa
    14761 cccccgacgc ctgtcgccgg tcggagccca gctcctcaac gacctggaac gggagcggcg
    14821 agcgacactg ctgtactcgt cgcggcccca gcagatcgcg gacgtcgacg ggttcccgat
    14881 ggcgtacttc aacgaccgcc ggacccccga cctccagttc gaccacgtcg tgttctcctc
    14941 ggggcggcga gcggtcggac ctgccgacgt gggcgcgagc gacgcggcac tgggcaccgg
    15001 ccgcctctgg ttcctcggcc aggacgacga aacccccgag ggcgcgccga gcgctcctgg
    15061 tgcctcgatc ggccgggacc tggtcaccgc cgtgttcccc gacgccgcca tccccgcccc
    15121 cgagccgtcc gcgtggagcc gccacgaggt gcccggggtg accgaggagc tgcgccagga
    15181 gcactacaac gccaccatca cccacttcga gccgacccac tcggacctgt gggtgctgcg
    15241 ggtccgcccg gaccacggat cggtgtcgca cctgcccggc cagtacgcgt cgctcgggct
    15301 cggctactgg gaggagcgcg tcgacgacgc gatcgaggcc aacatcgacg agcggtggga
    15361 caagctcgtc cgtcgctcgt actcgatcag ccaccggatc ttcgacgagc acggctacct
    15421 ggccgacgag gtcggatccg acgagctcga gttctacatc gtgctggtcc cgccgacgcc
    15481 ggacaacgtg cccggcctga ccccgcgact ggcgctcaag aagcccggcg accgcatcta
    15541 cctgggtccc aaggtggccg gccgttacac gttggcgccg gtcaccgacc ctgccgacac
    15601 cgtggtgttc ctgtccaccg ggaccggcga ggcgccgcag aacgcgatgg tcaccgaact
    15661 gctgcgcaag ggccaccacg gcccgatcgt gtcggcggtg tcggtgcgtc agtgggccga
    15721 cctcggctac ctcgaccagc accgccggct cgagcagcgc ttcgccaact accactacgt
    15781 cccgctgccc acccgtgagc ccgacgtccc caagcgctac atccaggacc tgatcgccgg
    15841 cgacgtgttc gccgccgatc tcggcatcac cctcgacccg cagaacaccc acgtgttcct
    15901 gtgcggcaac ccggcgatga tcgggctgcc cgagggcggc gacgacgggg accagacctt
    15961 ccccgagacc gtcggcgtgg tgcagctgct ggtcgaacgg ggcttcaccc tcgatcaccg
    16021 caagaagcgg ggcaacatcc actacgagga gtactggtag accgacgccg tcgcccgggc
    16081 cggaaccggc gacgccgaat ccaggggggc agaacatgaa ggtctcggtg tcggtgggca
    16141 gcgcctacta cgacgggtcg cgctgggagg acctggtcgc ctacgtggcg gccgccgaac
    16201 ggctgggagc cagcacggtc tggtcggccg aggcgtgggg gatggacgcg gtcgcgtcgc
    16261 tcgggtacct ggccgcggtc accgaccgga tccgcctcgg ctcggggatc atgcagatca
    16321 gcgcccgcac cccggcagcg acggccatga ccgccctgtc tctcgcgcag ctgtcgggtg
    16381 accgcttctc gctcgggctc ggggtcagcg gacctcaggt ggtcgagggc ctgcacggcg
    16441 ccgagttcgc cgatccggtc gggcgtctgc gcgagtacat cgagatcgtg cggatgggcc
    16501 tgcgcggcga ccggttggcc tacgagggtc ggcactacgt cctgccgcga ccgggcggcg
    16561 agggcaaggc tctgcgatcc tcggttccgc cccgccccga tctgccgatc cacctcgcca
    16621 cgctcggacc gaggtcgctc gagctcaccg gcgagctggc cgacggctgg ctcggcacct
    16681 cgttcatccc cgagcaggcg tcggtgatgc tggaccccat ccgccgcggg gccgagcgga
    16741 ccgggcgggc gttctccgac atcgagatcg aggtcaacgc ccacctcgcc gtctccgacg
    16801 acgtcgagtc gttgatcggg atcgccaagt tcggcatggc cttctcgctc ggagcgatgg
    16861 gttccgcgac gacgaacttc tacaacaacg cgtactcccg ggccggcttc gcggacgtgg
    16921 cggaccgggt ccgacgcctg tgggtcgacg gcgacaagga cgccgcgatc gcggcggtcc
    16981 ccgacgagat ggtgctgtcg gcgtacctga tcggaaccga gtcgatggtc cgtgaccgca
    17041 tccgggccta ccgcgacgcc ggggtcacca ccctccggct cgggcccagc ggatcgaccg
    17101 cggccgagca gatcgaacac ctcgagatgg ccgtcgacct catcggttcg gcggcggcgg
    17161 actgaacgtg gcccaccccg gacaccacgc tgcggaccgg cccgacgaca ccgccgtcgt
    17221 ggtcctggga gacggggccg aaccggtcga gtccgccacc tgggccgagc tgcgcgcccg
    17281 tgccgtcgcc gtggccgacg atctgtgggg tctcggcctg cgagcgggcg acccggtcgc
    17341 gttctgcctg gagaaccggg tcgacttctt cgccctggtg tgggcgtgcc aggacgccgg
    17401 gttccgctac acgccggtgc cgacacggct gaccgccgag gaggtgcgcc acatcctctc
    17461 ggactgcggc gcccgcgtgg tgctgcactc ggcccggacc cgatccgtgg tcgtcccggc
    17521 gtgctccgag ctcgacgggg tcatccggat cgacctcgat cccggcgacg tcttcggtgc
    17581 cgggtcgggc gaggccccgc gctacgaacg ggtcgagggc gttgcgctcc tctactcgtc
    17641 ggggacgacc gggcgaccca agggcgtgtg gcgcgccgcc ccgccggagc cgatcgagga
    17701 gctgccgccg ggcgaccagc tcctggccgc ggcctacgga gtcgggcccg gctccgtgta
    17761 cctgtcgacc gcgccgctgt accactcggc acccctcacg ttcctggtgc agatgggccg
    17821 gatcggtgcg acgacggtcg tcatggaccg cttcgatccc ggcgccgcgt tggccgccat
    17881 cgagcgatac cgggtcaccc acagccagtg ggtgccgacg atgttcgtgc gtctgctgcg
    17941 gctcgacgag gccgagcgcc gggcccacga cctgtcgtcg cacaccttcg ccatccacgg
    18001 agccggaccc tgcccggtcc cggtcaagga ggcgatgctc gactggtggg gcccgatcat
    18061 ccacgagtac tacgccggga ccgagggcgc cggcacctgc gtgatcggtc cgcaggagtg
    18121 gctggcccgc aaggggtcgg tcggccgccc gatccggggc cgtgtgatga tcctcgacga
    18181 cgacggcacc gagctcccgg tcggacagga cgggcaggtg tggttcagcg agagcaccga
    18241 cttccgctac ctgcacgacg acgccaagac cgccggggtc cgccgggccg acgccgccac
    18301 cttcggcgac ctcggccatc tcgatgacga cggctacctg tacctcaccg gtcggcaggc
    18361 gttcacgatc aacgtcggcg gcgtgaacgt ctacccgcgg gagatcgagc tggtcctgct
    18421 cgagcacccc gcggtggccg acgtcgcggt cttcggccgg cccgacgacg agtacggtga
    18481 gcaggtggtg gccgtggtcg aggtcgccgc agggatcgag ccggacacgg tgctggaggc
    18541 ggacacggtg ctggaggcgg acacggtgct ggaggcggag ctgctctccc actgccggga
    18601 ccgactcgcc accttcaagc tcccccggac ggtgcacctg gtcgacgagc tgccccggga
    18661 accgaccggg aagctgcggg tcgcggacct gcaggcccgc taccgctgag cgggggagcg
    18721 gtcagccggc cggttcgagc acgaacacgg ggatcgtgcg gtccgtcgac gcctggtagg
    18781 tgtcgtagtc gggccagatc gcctggccgc gcgcccacca ctgggccttc tcgtcgccgt
    18841 cgacctcccg tgcggtcaga tcgtggaggt cggcgccgtc ctggatgcgg gcgacggggt
    18901 tggcccgcag gttgtgcacc cagttggggt tggtcggtgc cccgccctgg gagccgatca
    18961 ccgcgtagcg gccggctccg tcggcgatgc gcaccagcgg cgtcttgcgc accgacccgg
    19021 aacgggcgcc gatcgtccac aggatcatcc acgactcgcc gaccagggcg ctcggctcgg
    19081 tgccggcgga gctctcgtag cgggcgacct cctcggagat cggttcccac gggctcggct
    19141 cgtacggggc gtcgaaggcg gacatcggtt ctcctggtgt cgggcgggac ggacggctcc
    19201 gaggttcaag gtctccggcg tccgcgtcga ccaacggtag atcggacccg caccggcgcg
    19261 tgtccacgag ccacggacga ccacatggga cctgaccaag caccgggcag ctcggactgg
    19321 atggtcccca tcggaatcct ggccgggctg ctcggaacgg cggccgccgg ggtcggtgcg
    19381 ctcatcgccc tgctctccac caactcgtgc ggcgccttcg ccgacagctg cgacacctac
    19441 gggcagccgg ccgaggagtt cctccccgcc gtcggggtga tggccctggg cgcggcggca
    19501 gcggtggtcg gattcgttct cctcgtcgtg gggatgtccc gggcggcccg agctcgacag
    19561 tcgtcggatc ctcgctctga acccggcccg cccctgctcc gctgagggat cagtccgccg
    19621 gaagggtcgg cggtggccag tcccgctggt cgatcggggc gaacaccggt tgggtggcga
    19681 cgcatcgggg ggtggtcgcg gtcgccggat ccaggaagtt gtcgatcacg tcgtaagcga
    19741 ccgggtcgaa cacgatcgac agatggtcgg cttcatcgat ggcgcagacg tcctggacgg
    19801 tcgtgttggt gacggccgga tggtcgatga agccgtactg gtacggcacc aggaagttgt
    19861 cgagctgggt ggccagcatc atgtagcgca cgccgggctg ggtcgggccg gtgaaccgtt
    19921 cgccgggctg gggttccgac gagttcagct gcttcaggaa cgacgacccc gacaggacct
    19981 gctcgcaggc cccgcagccg ttctgcacca tcgtgtcgat caccccggcg aagaacggga
    20041 tcgaccgcag ggtggcggcg ttgccgccga ggtcggcacc gtgggtggcc ggtccgaccc
    20101 cgacgaacgt cgcgaccttg ggcgtgccgt cggaatgcac cgacggaccg aatcgcatcc
    20161 accagcgggg catgatcgac ccctcggagt ggccgaccac gtcgacctgc cgggcgccgg
    20221 tgcgggcgag cacgtcgtcg acgaacgggc cgaactcctc catcgaggag cggcggatgt
    20281 cgtgcagccc gccgaacagc gaaccgtccg ggtcgccgta ggtgagggcg aacacgcagt
    20341 agccctggtt ggcgaggaac ggggagatgc cgcccatgtt gtcggccatg ttgccggcga
    20401 agccgtgcag gaggatcacc ggtcgggggt gggcggtgga cgggacgcag ccgtcgacgt
    20461 tggcgcccgg gggcgggccg tggatgtcgc ccagggcggt caacaggcct tcggcgaagt
    20521 cagcggggac ccgatatcgc tggcccggct ccggcgccgg ggcgcacgcc gtcacgagca
    20581 gcgccgccgc ggcgagggcg agcgccgccg ctgtcaggcg tcgtggacgc cgccgggccg
    20641 tcgagccgct ccgcttcatg aactgtcccg ccatcatcgt ccccccacga ttccgcccca
    20701 ccagaggccg gaaccacggt accaccggcc tccggcggcg gtccgggcgg cctcgatcgc
    20761 ggcgtcagag cgcgacgacg tgtccgggca cgtccacccc gagggcggcg tacccggcgc
    20821 gcagataggc ctctccggtc ggcgagccga cgatgccggc ggccatcttg ttcatcgtgt
    20881 aggagaacgt cgtccgacgg tcgagatcca tgatgatgaa cgacccaccc cagccgcccc
    20941 agtgcagcat cc
//
LOCUS       DAOQOD010000038        20916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_872_length_20916_cov_11.031111, whole genome shotgun sequence.
ACCESSION   DAOQOD010000038 DAOQOD010000000
VERSION     DAOQOD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20916
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_20916_cov_11.031111"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLZ72_05725"
     CDS             complement(<1..667)
                     /locus_tag="PLZ72_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNA35831.1"
                     /translation="MHDAGPILIRDVEDPRLADYRHLNDAAARVAAGRDDTTGGGCAI
                     VEGAVALEVVLRAGIPLRSLLLTRTRAESMAPLLERHAADVPLFVADRDVLAEVVGFD
                     LHRGVLASAERRPPPPADEVLGSCSRVVVTEGLNDHENLGALFRNAAALRIDAVLLDD
                     RTADPLYRRCIRVSSGWAAVLPHARLGALPGGYDRLRSHGFRVVALTPSPDALAADRA
                     AAAG"
     gene            complement(708..4220)
                     /locus_tag="PLZ72_05730"
     CDS             complement(708..4220)
                     /locus_tag="PLZ72_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA35832.1"
                     /translation="MEATPGTGMAEHHHHAHIGASESLLATRHHAIRDYVIGAVAWYA
                     IFAALPNDLKGPWFVVGGFTTAIVSITRALRIPPPVRRPAILMVAAGFCSLTGGVVRG
                     VESMLTGNPYPFPSTADVFVVTSYLLLLAAMILVVAERLPRFSIDPLLDAVVAAIAVG
                     VVQWSVILIPHLRDASATTGALIINLLYSAMSLVLIGIAVFALVAGGHRSTANRLLAT
                     AFVMVVLVDWIATLVTGGVIDESVRLWAAPVPLILGTLGLMQPSVVLVASRPTDPSAL
                     RILTRKRIAVLALALLTCPTLLLVFAALGQSNRMILPAVASIALAPLVMVRLGRLVGQ
                     NEMLAALEATLRSVGERLVAAETEEDVVRIITVGLEQVLGARLLDGGLILDPTDRRRR
                     PDTGSLTPAIRALDPGSFINPRSLRTGEVRLVDTNEPGQWYLANVVLQRELRALLVAV
                     SRSEISDDEHNALQSLSREASIALRAVEQTEINVRQRSEERFGSLVDNSSDIVAIMDD
                     HGRLSYVSPVAARLLGYPSDFRGIDHVLDLVHPDDRDAAARMLDDVRFGKRVPIEVRL
                     RHVAGTYHWFEVLGVDLQSDPNIAGIVLTAREIGDRKAAEQQLQLSEARFKALVQNSS
                     DVVLVVGANGTVRYASPSVVSTMGLSPDELMGRTLEEAFRDSGVDWEVALRSGHDQGE
                     HPELLEFGFRNERSDWIHMEASVTDLRREEAVGGFVLNARDVTERTTMMQRLRYQSTH
                     DALTGLANRVLAAEELGGMLSRNAGGSTVAVISLDIDDFKDVNDSLGHGVGDRLLYAV
                     ADRIKESLAFGDIAARSSGDEFVVVLERAHGEAQVLELADRLLSSLERPFDVDGRELT
                     VTASAGVAYDHDRETAAEVLLRNADTAMYKAKQMGKRRSVVFEPHMHTASADRLELRA
                     DLARAIDTEQFVAHYQPIVDLLTRRIVGCEALIRWQHPQRGLLSPAVFVPLAEESGLI
                     GAMGEWMMERACRDLSEWRSSMPEVAEAMTISVNLTAQEVHGERLVPVVTDILRRTGL
                     PADRLVLEVTESNLLSDTQVIQDRMQRLRELGTRLAIDDFGTGYSSLGYVQKFAFDVL
                     KIDRSFIEGLDRQTNRQIVTAVLDLARELQVRVVAEGIEEEDQEQALIELGCVYAQGY
                     RYSRPVPAAQFRKLLIEQTAPARA"
     gene            complement(4305..4823)
                     /locus_tag="PLZ72_05735"
     CDS             complement(4305..4823)
                     /locus_tag="PLZ72_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35833.1"
                     /translation="MIDVSGNDVIRTPVATINEQGGPFIGSTALSDQKIGWVQDLRSL
                     GLFGRAWAVGVVLFSVARALLTWPTLSTYGVDPWVFLAIDVITAPPYGVGQALTVKIL
                     RDPSRHTRDATGWAALVVVMFLAPYIYILMASGERMPLAAYVLVALWALLFGTLAIVR
                     MRKQVLADDDLA"
     gene            complement(4820..5539)
                     /locus_tag="PLZ72_05740"
     CDS             complement(4820..5539)
                     /locus_tag="PLZ72_05740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA35834.1"
                     /translation="MAQTATPTIVGGSTGLAVITDDPLYTFTPEKLDPELVSEHTTPE
                     TDIGRVAERFVYEIYRVSGFCKESERGVVEETEPWRPGSALHIVRADDQVLGTVRTLL
                     GTYQQLPIGQFEPIVTPPTGRLCEVASLAVKPDRRGMGVANELHRMTFEFGVRQQVEG
                     FCFLVDSWMVTFFEDYYAFPVTRMAPIQDYMGGQIEPIYVPMDQLLIDFPSRRPFAYE
                     VAISGFTPQEIVDFDLPIILR"
     gene            5644..6606
                     /gene="hisG"
                     /locus_tag="PLZ72_05745"
     CDS             5644..6606
                     /gene="hisG"
                     /locus_tag="PLZ72_05745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNA35835.1"
                     /translation="MSISGNGRERAVALLFGFGRPSRSDYPRRVLKLVLPKGSLERST
                     MDLFEAADLPVRRDSSVSYKATIDDPRIESVRILRPQEIPTYVADGLFDLGITGRDWV
                     EETGSQVTSLGELRYSKATTNPITVVVAVPGDSEYRSVSDLPSGVRVSTEYPELTRRF
                     FADKGVDADIRLSYGATEAKVPDIVDCVVDITETGSALRAAGLRVIDVILVSYTELVA
                     NPAAFADPDKRHAMEQVLTLLSGVLEARGKVLVKLNVATDALDAVIEVLPAMKTPTVN
                     ELYGSAGYAVETVVPKNEINILIPALKDAGATDIIELPLSKIVH"
     gene            6636..6857
                     /locus_tag="PLZ72_05750"
     CDS             6636..6857
                     /locus_tag="PLZ72_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35836.1"
                     /translation="MGAAPLEATVVEFDREVGLGVVVTDDGERLGFHSTAIADGSRDI
                     AVGSRVLIGLAAAHAGTVEASPVTPVPGT"
     gene            complement(6917..7396)
                     /locus_tag="PLZ72_05755"
     CDS             complement(6917..7396)
                     /locus_tag="PLZ72_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35837.1"
                     /translation="MSSLWTPDGERPVARKPAPESTTSSGAASAPIDPADTDPGADPL
                     RAAAAALGLDLDAMTEEERTQLAAELEEMMQVRRQVAATPASEMLTNYLMRFFDLATI
                     YLEAEPPAFADAATVIEAFRSVLDGVGDRLGEHEPLLREALGQAQMVFVQVKNAAGA"
     gene            7765..8283
                     /gene="infC"
                     /locus_tag="PLZ72_05760"
                     /pseudo
     CDS             7765..8283
                     /gene="infC"
                     /locus_tag="PLZ72_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            8479..8673
                     /gene="rpmI"
                     /locus_tag="PLZ72_05765"
     CDS             8479..8673
                     /gene="rpmI"
                     /locus_tag="PLZ72_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNA35838.1"
                     /translation="MPKMKTHSGAKKRYKVTGSGKILRRNSNLNHILEKKSPARKRRL
                     AGTTELTGGDRNRARRQLGL"
     gene            8782..9159
                     /gene="rplT"
                     /locus_tag="PLZ72_05770"
     CDS             8782..9159
                     /gene="rplT"
                     /locus_tag="PLZ72_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNA35839.1"
                     /translation="MARVKRGVHAKKKHRAVLNKAKGYYGHRSRTFRAANEAVMHAGN
                     YAFRDRRARKGEMRKLWIQRINAACRLNDISYSRFINGLKLAEVDIDRKALADVAVRD
                     PQAFAALVEVARAAVDAAPASAS"
     gene            9315..10139
                     /locus_tag="PLZ72_05775"
     CDS             9315..10139
                     /locus_tag="PLZ72_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNA35840.1"
                     /translation="MLLAEALAAGVEIRSVFVEPAGQDLPAVRTARGAGVDVHDVLDG
                     ALARVLDMPTPQSVVAVAAADPVQADAVLEQARSARRPVMVLVEVQDPGNAGTLVRVA
                     EAAGCAGVVLTERSVDLHNPKTVRATAGALFRLPVAEGADALRLVSAADGMGIPSWAT
                     VRDGGTPIDAVDLRSGGLLLVGSESHGLPTEVVAASTGRLSIPMEGAVESLNAAVAGA
                     LVAFEAARQRRVGLGTGPGPTDREPVRGSAVALGHDVSSPVGDGGGGSDGVEERSR"
     gene            10136..11179
                     /gene="pheS"
                     /locus_tag="PLZ72_05780"
     CDS             10136..11179
                     /gene="pheS"
                     /locus_tag="PLZ72_05780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNA35841.1"
                     /translation="MSGAELAEETRTLVAAGLARLADAPSSAEVRSIESELLGKRSRL
                     TEMKAGMRDLDAEDRRVLGAALNQARTELEAAAAERLDHLAARERRAVLAAERLDLTE
                     YQGRASTGHRHLVTQTWERLEDVFVGMGFTVATGPEVETDWFNFEALNLPPSHPARSL
                     WDTLYLDTSDLDGVGDQQLLLRTHTSPVQVRTMLRAVREGSGPPIYVVCPGRAYRQDT
                     ADATHLPVFHQIEMLVIDRGITMGDLSGTIETFTKAYFGQELTSRLRPSYFPFTEPSA
                     EVDISQPDGSWLEVGGCGMVHPNVLRAGGLDPEEWSGFAFGFGIERLTMIRHHVTDIR
                     DFITNDMRFLEQF"
     gene            11215..13608
                     /gene="pheT"
                     /locus_tag="PLZ72_05785"
     CDS             11215..13608
                     /gene="pheT"
                     /locus_tag="PLZ72_05785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNA35842.1"
                     /translation="MRVTLNWLREFAPVEGSPHDIAEQLTDLGLEVGEVTPVGGALGG
                     VIVARVLEVREHPDADRVRLVDVDTGDGEALQIVCGASNMAAGDLVPLATVGTVMPGG
                     MEIAARKMRGQMSNGMLCSAQELELGDDHSGILILPAEEVPGVPLTEALGLSADVVFD
                     LEVNPNRPDALSVAGVARDLAARQGVPFTIPTPAPVGSGPTFDGAVSVTINDPHLCGR
                     FALRGLGGVRIGASPRWMAQRLIAAGMRPINNVVDASNYVMLELGQPTHAFDLDKVRG
                     AQLRVRRVRAGEQLETLDGVLRDLDPADGVIVDGDDVPIALAGVMGGASTEISDTTTD
                     VIVEAAWWDPLSVADTSARHNLHSEASARFKRGMDPDVADRALDRLAELLVRQDAATL
                     RPGSVVAEGNLPERVTVAVRPARVNAVLGLSLDRSTMVDLIDPIGFTADIVGDDLSVA
                     IPSWRPDSTAEVDVIEEIGRMYGLSRIPRTVPVSAHPGRLDAAQRARRSVRRALAGFG
                     VSEAMPMPFLAPGDLERCGLPAGGLVLANPLAAEESVLRSSLRPGLLSAIAYNASHRR
                     PGVWLAEIGRVFETGERGVVVDVAESARLGRVLDGERDQLAAALAGAEAPTAVELLDV
                     VLGSLGVGPVVLRAEERPGLHPARTAVVEVAGLDAGMVGEIDPEVLERLGIEERVAWL
                     ELELGALLELVAPVRRARAVSRFPSTDVDLAFVVEDRVPAAAVRTTIRAVGAPLLQDL
                     DLFDVFRSESLGADRRSLAFRLRFQAEDRTLTDAEVGVVRQAIIDEVVATHDATLRA"
     gene            13731..14762
                     /gene="argC"
                     /locus_tag="PLZ72_05790"
     CDS             13731..14762
                     /gene="argC"
                     /locus_tag="PLZ72_05790"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNA35843.1"
                     /translation="MAGDTARVGIIGASGFTGAELLRICAQHPGLDVAFATGDSQAGT
                     AAADLYPGLAPAYPDLVFQAWDPALLDGVDGVFVGLPHGASQTLMADVLGKVRTVVDL
                     GADFRLSDPADYQRWYHEPHTAPELLDRFVYGLPELYRDRIEGADAIAAPGCYPTATT
                     LALRPLVDAGLIHTEGIIVDAVSGVSGAGRPPKPHTTFCAVDENVEAYGLLDHRHTAE
                     MDMNLGATVLFTPHLVPMNRGILATCYARPAVDGLSTDAVLDVLAQRYADEPFVVAST
                     RSPSTKATLGTNAAHVTARYDARTGTVVSIAAIDNLGKGASGQAVQCMNLALGLPETQ
                     GLAVVGTAP"
     gene            14759..15691
                     /gene="argB"
                     /locus_tag="PLZ72_05795"
     CDS             14759..15691
                     /gene="argB"
                     /locus_tag="PLZ72_05795"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNA35844.1"
                     /translation="MSPEASGAPSGGLDPRRDPDLAVQVLLQALPYIEAFRGAVVVVK
                     FGGNAMGDPDLFEQFASDIVLMHRVGMRPVVVHGGGPQIGEWLDKLGKASEFVDGRRV
                     TDAETLDVAQMVLIGKVNADIVTALNGFGPLALGLAGTDAHLIEAEAHDPELGFVGAV
                     TRVNPALITRTLAMDLIPVIATIGVDRTGQTYNINADDAATAIAEELGAQKLIFLTDV
                     PGLLADAEDPASLITRVDTDEVARLIDDGTISGGMVPKMSGCARAVGGGVGSVHLIDG
                     RRPHVLLLELLTDAGVGTMVVPAAAAAADEGETS"
     gene            15688..16860
                     /locus_tag="PLZ72_05800"
     CDS             15688..16860
                     /locus_tag="PLZ72_05800"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HNA35845.1"
                     /translation="MTAPSPLMSTYPPSPVTFVRGEGSWLYDDTGRAYLDLLSGLAVT
                     SLGHSHPDVADALAEQARTLLHVSNLFGTVPGPQVAATLDRLLGGGGQVFFANSGAEA
                     NECAIKLARRWAGAPAGRYKVVSALGSFHGRTLATLHATGQPAKHEPFQPLPDGFVHV
                     AWDDLDALAAAIDPSVAAVLLEPVQGEGGVNPATVEYFRGVRDLCDERGVLFMVDEVQ
                     TGLGRCGAWFAHQHFGVVPDVVTMAKALGNGVPIGACWARADVAAAFEPGDHATTYGG
                     QPLATAAARAVLAVMEDRDVPARARHIGEVVASELTAAPGVLDVRGLGALIAVEVAVD
                     ARQLCSELLGRGVVVNAVTPTALRLAPSLLIDDAELRSGTSSIAETVAEILEGDEN"
     gene            16860..17801
                     /gene="argF"
                     /locus_tag="PLZ72_05805"
     CDS             16860..17801
                     /gene="argF"
                     /locus_tag="PLZ72_05805"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNA35846.1"
                     /translation="MSANPPLRHLLEVDDLSAVELARILDLAELTDPPPVLAGSGGVL
                     LFEKPSARTRTSMEMAIDQLGGHPVSLSGSEVGIDTRETAEDLGRLMSGYGRVIGARV
                     FEHHKLERMASVASVPVVNLLSDDAHPVQTLADLLTLRQCFGRLDGLTVTYVGDANNV
                     ARSLAIGCGLVGAAFRISSPPGYRFDDTSTDRIRSAGTDLTVVDEPTEAASGADAIYT
                     DAWYSMGQEEEKRIRQEAFARWRVDESLMDAAGDGAVFLHCLPAHRGDEATDGVLDGP
                     RSRIWPQAHNRMHSARGLLLWLAELSADQDPERGGPS"
     gene            17801..18265
                     /gene="argR"
                     /locus_tag="PLZ72_05810"
     CDS             17801..18265
                     /gene="argR"
                     /locus_tag="PLZ72_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNA35847.1"
                     /translation="MAGKQQRQHRIGRLLVEHRVTNQAQLTELLAADGVTVTQATVSR
                     DLEELGAIKVRVPGGDTVYAIPELPREQVSPQDHLRRVLGDWLVEVERSGDLVVLRTP
                     PGSAHVVASAIDRNGLDGVVGTVAGDDTLLVIAREGQGDPVALQLQQLAGLA"
     gene            18334..19530
                     /locus_tag="PLZ72_05815"
     CDS             18334..19530
                     /locus_tag="PLZ72_05815"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNA35848.1"
                     /translation="MAKRVVLAYSGGLDTSVAVRWMIENLGVEVVCLSADVGQEGTLE
                     GNREKALGAGAIAYEELDLRQEFADDFLAPLIKANALYEDQYPLVSALSRPLIAKKQV
                     EIARKYGADGVAHGCTGKGNDQVRFEVSYMALAPDLEQLAPVRNWGFTREDSIEYAAT
                     HGIPVGATKEKLYSIDDNLFGRAIECGEMEDPWAEPPAGVWKMTERTATEPAEFTLSF
                     ERGVPVALDGEARPLFEIVIALNHLVGSYGWGRLDMVENRRVGIKSRETYEAPGALAI
                     MLAHRDLESLTVERDLGRQKIALQHRYAELVYDGLWYSPLRDALDAFVDVSQQHVTGD
                     VRLRLEPNRCWVVGRRAPKSLYDYGLATYDAADRFRHADSEGFVRIWGLGVQTWSDVQ
                     GPGSQR"
     gene            19527..>20916
                     /gene="argH"
                     /locus_tag="PLZ72_05820"
     CDS             19527..>20916
                     /gene="argH"
                     /locus_tag="PLZ72_05820"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNA35849.1"
                     /translation="MTEDGSPADRGQLWHGRFDVPPAEALVEFTESLSYDQRLLPDDV
                     ACSKAHVRGLHRAGLIDEGELAALLGGLDSVEMEYRTGVLDFVASDEDVHTAIERRVT
                     ELVGPVGGKIHTGRSRNDQVATAFRHHVVGQMAATAELVLGLVDVLADRADRSLTGGV
                     DGDPVRLPGYTHLQRAQPVLLAHHLMAHAWALLRDVDRLADCRRRTHVSPLGAGALAG
                     SSLPLDPDATAADAGFPARFENSLDATSDRDFVAESLFVCTTIGLHLSRMGEEIVLWT
                     SDEFGFAVLDDTYATGSSMMPQKKNPDVAELARGKAGRLIGDLTGFLATMKSLPLAYN
                     RDLQEDKEPLFDALDTVRLALLAMTGLYATITFRPEPMAAAADSPYAAATDLAELLVT
                     DGIPFRSAHGIVGALVRRSLDEGIPLVDLVAAEPELGERGVALLRPGAGTANRASPGG
                     GGPEPVAAQRGRL"
BASE COUNT         3150 a         7331 c         7386 g         3049 t
ORIGIN      
        1 acccggccgc ggcggcccgg tccgcggcga gcgcgtccgg tgacggggtg agcgcgacca
       61 cccggaaccc gtggctccgc agccggtcgt agccgccggg cagcgccccg aggcgggcgt
      121 ggggcagcac cgcggcccag cccgacgaca cacggatgca tcgtcggtac agcggatcgg
      181 cggtgcggtc gtcgagcagc accgcgtcga tgcggagcgc ggcggcgttg cggaacagcg
      241 cgcccaggtt ctcgtggtcg ttgagaccct cggtcaccac cacccgcgag caggacccca
      301 gcacctcatc tgcgggtggg ggcggccgac gttcggctga cgcgaggacc cctcggtgga
      361 ggtcgaagcc gacgacctcg gcgagcacgt ctcggtccgc gacgaacagc ggcacgtccg
      421 cggcgtggcg ctcgagcagc ggggccatgg actccgcccg ggtccgggtg agcaacaggg
      481 agcgcaacgg gatgccggca cggagcacga cctcgagggc gaccgccccc tcgacgatgg
      541 cgcagccccc gccggtggtg tcgtcgcggc cggccgcgac gcgggccgcc gcgtcgttga
      601 ggtggcggta gtcggccaga cgcgggtcct cgacgtcccg gatcaggatc ggaccggcgt
      661 cgtgcaccgg cccagtctcc cgcacccgac cgtcgtgggg atcccggtca ggcgcgggcg
      721 ggagcggtct gttcgatcag cagcttgcgg aactgggcgg cggggacggg gcgggagtac
      781 cggtagccct gcgcgtagac gcagccgagc tcgatcaggg cctgctcctg gtcctcttcc
      841 tcgatcccct cggccaccac ccggacctgc agctcgcggg ccaggtcgag cacggcggtc
      901 acgatctggc ggttggtctg gcggtcgagg ccctcgatga aggagcggtc gatcttcaac
      961 acgtcgaagg cgaacttctg cacgtagccg agcgacgagt agccagtccc gaagtcgtcg
     1021 atcgcgagcc gggtgccgag ctcgcgcagg cgttgcatcc ggtcctggat cacctgggtg
     1081 tccgacagga ggttcgactc ggtgacctcg agcaccagtc ggtcagccgg cagaccggtg
     1141 cggcggagga tgtcggtcac caccgggacc agtcgctcgc cgtggacctc ctgggcggtg
     1201 aggttgaccg agatggtcat ggcctcggcg acctcgggca tggacgaccg ccactcggac
     1261 aggtcgcggc acgcccgttc catcatccac tcgcccatgg cgccgatcag gcccgactcc
     1321 tcggccaacg ggacgaacac ggcgggggag agcaggccgc gctgcgggtg ctgccaacgg
     1381 atcagcgctt cgcagccgac gatccggcgg gtgagcaggt cgacgatcgg ctggtagtgg
     1441 gccacgaact gctcggtgtc gatcgcccgg gccagatccg cgcgcagctc caggcggtcc
     1501 gccgacgcgg tgtgcatgtg gggctcgaac acgaccgagc gccgcttgcc catctgcttg
     1561 gccttgtaca tggcggtgtc cgcgttgcgc agcaggacct cggccgcagt ctcgcggtcg
     1621 tggtcgtagg cgaccccggc ggaggcggtg acggtgagct cccgaccgtc gacgtcgaag
     1681 ggccgctcca acgacgacag caggcggtcg gcgagctcca ggacctgcgc ctcaccgtgg
     1741 gcccgttcga gcacgacgac gaactcgtcg ccggaggagc gtgccgcgat gtcgccgaag
     1801 gccaggctct ccttgatgcg gtccgcgacc gcgtacagca gccggtcccc gacgccgtgt
     1861 cccaggctgt cgttgacgtc cttgaagtcg tcgatgtcga gcgagatcac cgccaccgtg
     1921 ctgccgccgg cgttgcggct caacatcccg cccagctcct cggcggccag cactcggttg
     1981 gccaggccgg tgagggcgtc gtgggtcgac tggtagcgca gccgctgcat catggtcgtg
     2041 cgctcggtca cgtcgcgggc gttgaggacg aagccgccga cggcctcctc ccgacggagg
     2101 tcggtgaccg acgcctccat gtggatccag tcgctgcgct cgttgcggaa gccgaactcg
     2161 agcagctcgg ggtgttcgcc ctggtcgtgg ccggaccgca gggcgacctc ccagtccacg
     2221 ccgctgtcgc ggaaggcctc ctcgagggtg cggcccatca gctcgtcggg gctgaggccc
     2281 atggtcgaga ccaccgacgg gctggcgtag cggacggttc cgttggcccc gacgaccagg
     2341 accacgtcgc tcgagttctg gaccagcgcc ttgaagcggg cctccgacag ctgcagctgc
     2401 tgctcggcag ccttgcggtc cccgatctcg cgggcggtga ggacgatgcc ggcgatgttc
     2461 ggatcgctct gcaggtcgac gccgagcacc tcgaaccagt ggtaggtgcc ggcgacgtga
     2521 cgcaaccgga cctcgatggg cacccgcttg ccgaagcgca cgtcgtcgag catccgtgcc
     2581 gcggcgtcgc ggtcatcggg atggaccagg tcgagaacgt ggtcgatgcc ccggaagtcc
     2641 gacgggtagc cgaggaggcg cgcggcgacc ggtgacacgt aggagagacg gccgtggtcg
     2701 tccatgatcg ccacgatgtc ggagctgttg tcgacgaggg acccgaagcg ctcctcggac
     2761 cgctgccgga cgttgatctc ggtctgctcc accgctcgca acgcgatcga cgcctcacgg
     2821 ctcagcgact gcagggcgtt gtgttcgtcg tcggagatct cgctgcggga gacggcgacg
     2881 aggagggcgc gcagttcgcg ttgaagtacg acattcgcca gataccactg gcccggctcg
     2941 ttcgtatcta cgagtcggac ctcaccggtg cgcaggcttc gcgggttgat gaatgatccc
     3001 ggatcgagtg cacggatcgc tggggtgagc gacccggtat cgggcctgcg tcgccgatcc
     3061 gttgggtcga ggatcagccc gccgtcgagt agtcgcgcgc cgagcacctg ctcgagtccg
     3121 acggtgatga tccggacgac gtcctcctcg gtctccgcgg cgaccaggcg ctcaccgacc
     3181 gaacgcaggg tcgcctcgag cgcagccagc atctcgttct gacctacgag acgtcccagc
     3241 cggaccatca ccagaggggc gagggctatg gaggcgacag cgggcaggat catgcggttg
     3301 gactggccca gggcggcgaa cacgaggagc agagtcgggc acgtgagcag agctagagcg
     3361 aggacggcga tccgctttcg ggtgaggatg cgcagtgccg atgggtccgt ggggcgactg
     3421 gcgacgagta ccaccgacgg ttgcatcaga ccgagtgtcc cgagaatgag gggcaccggc
     3481 gcggcccaga ggcgaacgct ctcgtcgatc accccgccgg tgacgagggt cgcgatccag
     3541 tcgacgagga cgaccatcac gaaggccgtc gcgagcaggc ggttggccgt cgagcggtgt
     3601 ccgccggcta cgagtgcgaa aacggcgatg ccgatgagta cgagtgacat cgccgagtag
     3661 aggaggttga tgatcagtgc gccggtcgtc gccgacgcgt cccgaaggtg ggggatgagg
     3721 atgaccgacc actggacgac accgacagcg atcgccgcga cgacggcgtc gaggagcgga
     3781 tcgattgaga atcgaggaag ccgctcggcc accaccagga tcatcgccgc gagcaacaga
     3841 aggtaggagg tcacgacgaa gacgtcggcg gtgctgggga acgggtaggg gttgccggtg
     3901 agcatcgact cgacgcccct gacgacgcca ccggtcagac tgcagaatcc agctgcgacc
     3961 atcaagatgg ctggacgccg caccgggggc gggatcctca gtgctctcgt gatcgacacg
     4021 atcgcggtgg tgaatccgcc gacgacgaac cacgggccct tcaggtcatt gggcagcgcg
     4081 gcgaagatcg cgtaccaggc gacagcaccg atgacgtagt cgcggatcgc atgatgccgt
     4141 gtggcgagga gtgactccga tgcgccgatg tgtgcgtggt gatgatgctc cgccattccg
     4201 gtccctgggg ttgcctccac ggaaccatcg gcgcgttgct accggaactg agccattgcg
     4261 acctcatcac cgggtgagaa cccggctagg agggccagac gccgtcaggc caagtcgtcg
     4321 tctgcaagca cctgcttgcg catccggacg atcgcgagag tgccgaacag cagcgcccac
     4381 agggcgacga gaacgtatgc ggcgagcggc attcgttctc cggacgccat caggatgtag
     4441 atgtagggcg cgaggaacat caccaccacg agggctgccc atccggtggc gtcacgagtg
     4501 tgccgtgagg gatcacggag gatcttcact gtcagcgcct gacccacgcc gtaaggcggc
     4561 gccgtgatca cgtcgattgc caggaagacc cacgggtcga cgccgtaggt gctgagcgtc
     4621 ggccaggtca gtaatgccct ggcgaccgag aacagaacga cgcccactgc ccaggcgcgg
     4681 ccgaacaggc cgagggaacg gaggtcctgg acccaaccga tcttctgatc cgacaacgcc
     4741 gtgctcccga tgaatgggcc cccctgctcg ttgatcgtag cgacgggtgt cctgatcacg
     4801 tcgttcccgg acacgtcgat caccgcaaga tgatcggcag gtcgaagtcc acgatctcct
     4861 gaggggtgaa cccggagatg gcgacctcat atgcgaatgg gcggcggctg gggaagtcga
     4921 tgagcagctg atccattggc acgtagatcg gttcgatctg gccccccatg tagtcctgga
     4981 tcggtgccat acgggtgacc gggaaggcgt agtagtcctc gaagaacgtc accatccacg
     5041 agtcgaccag gaagcagaag ccttctacct gttggcgcac gccgaactcg aaagtcattc
     5101 ggtgcagctc attggcgacg cccatccctc gtcgatcggg cttcacggcc agggatgcca
     5161 cctcgcacag gcgtccggtc ggtggagtca cgatcggctc gaactggccg atcggcagtt
     5221 gctggtaggt tccgagcagg gtgcgaaccg tcccgaggac ttgatcgtct gcccgtacga
     5281 tgtgaagagc ggagccgggc cgccatggct cggtctcttc cacgacacca cgttcggact
     5341 ccttgcagaa ccccgaaacc cggtaaatct cgtagacgaa ccgctcggcg acccgaccga
     5401 tgtccgtctc gggagtcgtg tgttccgaaa cgagctctgg gtcgagcttc tcaggtgtga
     5461 acgtgtacag cgggtcatca gtgatgacgg ccagcccggt cgaaccaccg acgatggttg
     5521 gagttgcagt ctgcgccacg tgccttgccc cttcaggccg ggctcttccc cagctccccc
     5581 cgacggaggt gagccgggcg gcagttcagc acatgggagg tgggtcgcgc taggagaact
     5641 tcggtgagca tctcaggaaa cggtcgcgag cgcgccgtgg cgctgctatt cggcttcggc
     5701 cgtccgagcc gctccgacta ccctcgccgc gtgctgaagc tcgtcctccc gaagggttcg
     5761 ctggaacggt ccaccatgga cctgttcgag gcggccgacc tgcccgtccg tcgtgattcg
     5821 tcggtgtcgt acaaggccac gatcgacgac ccccggatcg agtcggtgcg gatcctgcgc
     5881 ccccaggaga tcccgaccta cgtcgcggac ggcctcttcg acctcggcat caccggtcgg
     5941 gactgggtcg aggagaccgg ttcccaggtg acgtcgctgg gtgagctgcg ctactcgaag
     6001 gcgacgacca acccgatcac ggtcgtcgtc gcggtgccgg gcgactccga gtaccggtcg
     6061 gtgtcggacc tgccgtcggg ggtgcgggtg tccaccgagt accccgagct gacgcgccgg
     6121 ttcttcgccg acaagggcgt cgacgccgac atccggctct cctacggcgc caccgaggcc
     6181 aaggtgcccg acatcgtcga ctgcgtcgtc gacatcaccg agaccggctc ggccctgcgc
     6241 gccgcggggc tgcgggtcat cgacgtgatc ctggtgtcgt ataccgagtt ggtcgccaac
     6301 ccggcggcct tcgccgatcc cgacaagcgc cacgccatgg agcaggtcct cacgttgctc
     6361 agcggcgtgc tcgaggcgcg gggcaaggtg ctggtgaagc tcaacgtcgc caccgacgcc
     6421 ctcgacgcgg tgatcgaggt gttgcccgcc atgaagaccc cgacggtcaa cgagctgtac
     6481 ggctcggccg gctacgcggt cgagacggtg gtgcccaaga acgagatcaa catcctgatc
     6541 ccggcgctca aggacgcggg cgccaccgac atcatcgagc tcccgctgtc caagatcgtc
     6601 cactgagacg gtccgcgacg tacgcgatcc cgacgatggg cgctgcaccg ctcgaggcga
     6661 cggtcgtcga gttcgaccgt gaggtcgggt tgggcgtggt cgtcaccgac gacggagaac
     6721 gcctcgggtt ccactcgacg gcgatagcgg acggcagtcg cgacatcgcg gtcggctcgc
     6781 gggtgctcat cgggctggcc gcggcgcacg ccggaaccgt cgaggcctcc ccggtcaccc
     6841 ccgtccccgg aacatagggg tcgcaaccga caggacctgt cggttccgcg ccccggaacg
     6901 cttcggggac gcccggtcag gcccccgccg cgttcttgac ctggacgaac accatctgcg
     6961 cctgtccgag cgcctcgcgc agcagcggtt cgtgttcgcc caagcggtcg cccacgccgt
     7021 cgaggacgct gcggaacgcc tcgatcaccg tggcggcatc ggcgaacgcc ggcggctcgg
     7081 cctcgaggta gatcgtggcc aggtcgaaga agcgcatcag gtagttggtc agcatctccg
     7141 acgccggggt cgccgcgacc tggcgacgca cctgcatcat ctcctcgagc tcggcggcca
     7201 actgggtccg ctcctcctcg gtcatcgcgt cgaggtccaa cccgagcgcg gcggcggccg
     7261 cgcgcagcgg gtccgcgccg gggtcggtgt cggccggatc gatcggagcc gatgctgccc
     7321 cgctcgacgt ggttgactcc ggcgccggct ttcgggcgac gggccgttcg ccgtcgggtg
     7381 tccacagcga gctcatgggt gtgctccagg tcggtcgggg ggaagggtcg agggaggtcc
     7441 cggcggtggc ggtcggcgtg ccgatcggca cccggacctg accgctccgg cctcggctgg
     7501 tacgctactg cgcacaaccg aggcaagcgg gctcccgccc ctcccggcca cgacggatcc
     7561 ggtcccggcc cagccggcga cccaccgtga cggtgcgccc gggattcgtc gattccgacc
     7621 ctccccaccg ggtgggccga cgacgtcatt gcgggtgccc gagggcatcc gcttctttcg
     7681 ttttcgaccc cggtcgattc cgatggcggg ctcggcaccg acagaccacc gaggaaagga
     7741 actgctgttg agcagtgatc ggccatagct gccgcatcga acgacgagcc ccgcatcaac
     7801 gatcgcatcc gcgctcgtga ggtccgactg atcggacccg acggggacca gctcggcatc
     7861 aagccgctgc ccgaggccct ggccaccgcc cggtccctcg acctcgacct cgtcgaggtg
     7921 gcggccgagg ccaacccgcc ggtgtgtcgg atcatgaact tcaccaagtt caagtacgag
     7981 gcgcagcaac gggccaagga gtcccgcaag aaggccacca acatcgtggt caaggagatg
     8041 aagtaccgcc ccaagatcgg tggcggcgac ttcgacacca agacccgcaa ggtcgcccag
     8101 ttcctgggcg agggccacaa ggtcaaggtc acgatcatgt tccgcggacg ggagatgcag
     8161 caccccgagc tcgggcgccg catcctcgac cgggtggccg aagaggtcgc ccacctggga
     8221 cgggtcgagg tcatgcccaa gcaggacggc cgcaacatga ccatggtcct gggccccgac
     8281 aagaaggcgc aggccgccca cgccaagaag gcgaccgagg acggatcgga cggacccggc
     8341 gacgaggtcg cccccgtcgc ggatgccgtg gagagggctc cggccccggc cgccgagacc
     8401 tgaccccggt cgggtcagcc gcccgacgtc gccctgacgg gaccgacagg acaccgctcg
     8461 agagcacaag gaaccaccat gcccaagatg aagacccaca gcggcgccaa gaagcgctac
     8521 aaggtgaccg gaagcggcaa gatcctgcgc cgcaacagca acctgaacca catcctcgag
     8581 aagaagtccc cggcgcgcaa gcgacgcctg gccgggacga ccgagctcac cggcggggac
     8641 cgcaaccggg cccgccgtca gctgggcctc tgagacccgc cggagaccgc tccggcacca
     8701 ccccgtcccg cccgagtgtc ccctcggtgc gcaccgcaga tgtgcgcccc gacggggacc
     8761 accccgcaga aggagtcaca gatggcacgc gtcaagcgtg gcgttcatgc caagaagaag
     8821 caccgcgccg tactgaacaa ggccaagggc tactacggcc accggagccg cacgttccgg
     8881 gccgccaacg aagcggtgat gcacgccggg aactacgcgt tccgggaccg tcgggcccgc
     8941 aagggcgaga tgcgcaagct ctggatccag cgcatcaacg cggcgtgccg gctcaacgac
     9001 atcagctaca gccggttcat caacggcctg aagctggccg aggtcgacat cgaccgcaag
     9061 gcgctcgctg atgtcgccgt gcgcgacccg caggcgttcg ccgccctggt cgaggtggcc
     9121 cgcgccgcgg tcgacgctgc gcccgcctcc gcgagctgaa gcggatcacc ctgtcgccga
     9181 acgcggacga tcgttcgtcg gcccgtcccc gggccggcct gagcccgtcc aacccggcac
     9241 tggtcaacct gcggcgcctc tcgaggcgcc gcagggcgcg tcaggactcc ggcacgttcc
     9301 tggtcgacgg tccggtgctc ctcgccgagg ccctggcagc cggcgtcgag atccgctcgg
     9361 tgttcgtcga gcccgccggc caggacctgc cggcggtgcg aaccgcccga ggggccggtg
     9421 tcgacgtcca cgacgtgctg gacggtgcgc tcgcccgggt gctcgacatg ccgaccccgc
     9481 agtcggtcgt cgcggtcgcc gccgcggatc ctgtgcaggc cgacgcggtt ctggagcagg
     9541 cccggagcgc tcgacgtccg gtgatggtgc tggtcgaggt gcaggacccg ggcaacgccg
     9601 gaaccctggt ccgcgtcgcc gaggccgccg gatgcgccgg tgtcgtgctg accgagcgct
     9661 cggttgacct ccacaacccc aagacggtcc gagccaccgc aggagcgctg ttccgactgc
     9721 cggtggccga gggcgccgat gcgctcaggt tggtctcggc agccgacggc atgggcatcc
     9781 cgtcgtgggc cacggtccgc gacggcggca caccgatcga tgcggtcgac ctccgcagtg
     9841 gcggactcct cctggtgggc agcgagtccc acgggctgcc caccgaggtg gtcgccgcgt
     9901 cgacggggcg actcagcatc ccgatggagg gagcggtcga gtcgctcaac gccgcggtcg
     9961 ccggggcgtt ggtcgccttc gaggcggcac gacagcgacg ggtgggcctc gggaccggcc
    10021 ccggaccgac ggaccgggaa ccggttcgtg ggagtgccgt cgcgctgggc catgatgtca
    10081 gctctcccgt cggcgacggc gggggaggga gcgacggagt cgaggagcga tcccggtgag
    10141 cggtgcagaa ctggcggagg agacccggac cctggtggcg gccggcctgg cgcggctggc
    10201 cgacgccccg agctccgccg aggtgcgctc gatcgagtcg gagctgctcg gcaagcgctc
    10261 ccggctcacc gagatgaagg cggggatgcg cgacctcgat gccgaggacc ggcgggtgct
    10321 cggcgcggcg ttgaaccagg cccggaccga gctcgaggcc gcggcggccg aacgactgga
    10381 ccacctggct gcccgggaac gtcgggcggt cctggcggcc gagcggctcg acctcaccga
    10441 gtaccagggc cgggcgagca ccggtcatcg ccacctggtc acccagacat gggaacgact
    10501 cgaggacgtg ttcgtcggca tgggcttcac cgtggccacc gggcccgagg tcgagaccga
    10561 ctggttcaac ttcgaggcgc tcaacctgcc cccgtcgcac ccggcccgct cgctgtggga
    10621 caccctctac ctcgacacgt ccgacctcga cggcgtcggg gaccagcagc tcctgttgcg
    10681 gacccacacc tcgccggtgc aggtccgcac catgctccgg gcggtgcgtg aggggagcgg
    10741 ccctccgatc tacgtcgtct gtccgggccg ggcctaccgc caggacaccg cagatgccac
    10801 gcacctgccg gtgttccacc agatcgagat gctggtgatc gatcgcggca tcaccatggg
    10861 cgacctgtcc ggcacgatcg agaccttcac caaggcctac ttcggccagg agctcacgtc
    10921 gcgactgcgt ccgtcgtact tcccgttcac cgaaccgtcc gccgaggtcg acatctccca
    10981 gcccgacggc tcgtggctcg aggtcggcgg ctgcggcatg gtgcacccca acgtgctgcg
    11041 cgccggcggc ctcgaccccg aggagtggtc cgggttcgcc ttcggtttcg gcatcgagcg
    11101 gctcaccatg atccgccacc acgtgaccga catccgcgac ttcatcacca acgacatgcg
    11161 attcctggag cagttctgac atgcgggtga cgctgacatg agggtgacac tgacatgagg
    11221 gtgacgctga actggttgcg ggagttcgcc ccggtcgagg gcagtccgca cgacatcgcc
    11281 gaacagctca ccgacctcgg gctcgaggtc ggcgaggtga ccccggtggg cggagcgctg
    11341 ggtggggtga tcgtcgcccg ggtgctcgag gtccgtgagc accccgacgc ggaccgggtc
    11401 cgcctggtcg acgtcgacac cggcgacggc gaggccctcc agatcgtgtg cggcgcctcg
    11461 aacatggccg cgggggacct ggtcccgttg gcgacggtgg gcacggtgat gcccggcggc
    11521 atggagatcg cggcgcggaa gatgcgcggg cagatgagca acgggatgct gtgctcggcc
    11581 caggagctcg agctcggcga cgaccactcc gggatcctga tcctgcccgc cgaggaggtc
    11641 cccggggtcc cgttgaccga ggcgctcggg ctgagcgccg acgtggtgtt cgacctggag
    11701 gtgaacccga accggcccga cgctctgagc gtcgcgggcg tggctcgtga cctggccgcc
    11761 cgccaggggg tgccgttcac gatcccgaca cctgcgccgg tcggatcggg accgaccttc
    11821 gacggagcgg tgtcggtcac gatcaacgat ccgcacctgt gcggacggtt cgccctgcgc
    11881 gggctcggcg gggtccgcat cggagcgtcg ccgcggtgga tggcccagcg cctgatcgcg
    11941 gcggggatgc gtccgatcaa caacgtggtc gacgcgtcga actacgtgat gctcgagctc
    12001 ggtcaaccca cccacgcctt cgacctcgac aaggtgcgcg gtgcgcagct gcgcgtgcgt
    12061 cgggtcaggg ccggcgagca gctcgagacg ctcgacggcg tgctccgcga cctcgacccg
    12121 gccgacggcg tcatcgtcga cggagacgac gtcccgatcg ccctggccgg cgtgatgggt
    12181 ggcgcatcga cggagatctc cgacaccacg accgacgtca tcgtcgaggc cgcctggtgg
    12241 gacccgctgt cggtggccga cacctccgcc cgacacaacc tgcactccga ggcgtcggcc
    12301 cgattcaagc ggggcatgga ccccgacgtc gccgatcgtg cgctcgatcg gctggccgag
    12361 ctgttggtgc gccaggatgc cgcgacgctg cgtccgggct cggtggtcgc cgaggggaac
    12421 cttcccgagc gcgtcacggt cgccgtccgt cccgcgcggg tcaacgccgt cctggggctg
    12481 tctctcgacc ggtcgacgat ggtggacctc atcgacccga tcggtttcac ggcggacatc
    12541 gtgggcgacg acctgtcggt cgccatcccg tcgtggcggc ccgattcgac cgccgaggtc
    12601 gacgtcatcg aggagatcgg tcgcatgtac gggctgtcgc ggatcccgcg gaccgttccc
    12661 gtgtcggccc atccgggtcg tctcgacgcc gcgcagcggg cccgacggtc ggtgcgacgc
    12721 gcgctggccg ggttcggcgt cagcgaggcc atgccgatgc cgttcctggc gccgggcgac
    12781 ctcgagcgtt gcggtctgcc cgccggcggg ctcgtcctgg ccaacccgct cgcggccgag
    12841 gagtcggtgc tgcgcagctc gctgcgaccg ggtctgctca gcgcgatcgc ctacaacgcc
    12901 agccaccgtc ggccgggggt gtggctcgcc gagatcggcc gggtgttcga gaccggcgag
    12961 cggggcgtcg tcgtcgacgt cgccgagtcg gctcgcctcg ggcgggtgct cgacggcgaa
    13021 cgggaccagt tggccgccgc gctggccggt gccgaggccc cgaccgcggt tgagctgctc
    13081 gacgtcgtgc tcggttcgct cggggtcggg ccggtggtgc tgcgtgccga ggagcgaccg
    13141 ggtctgcacc cggcgcgcac cgcggtggtc gaggtcgccg gcctcgacgc ggggatggtc
    13201 ggtgagatcg accccgaggt gctggaacgg ctcggcatcg aggagcgagt cgcgtggttg
    13261 gaactggagc tcggggcgct gctggagctc gtcgctccgg tccgccgggc ccgggcggtg
    13321 agccggttcc cgtcgaccga cgtcgacctg gccttcgtgg tcgaggaccg ggtcccggcc
    13381 gccgcggtcc gcacgaccat ccgggccgtg ggcgcgcccc tgttgcagga cctggacctg
    13441 ttcgacgtgt tccgctccga gtcgctcggc gcggaccgtc ggagcctggc gttccggctg
    13501 cggttccagg ccgaggaccg gacgctgacc gacgccgagg tcggcgtcgt gcgccaggcg
    13561 atcatcgacg aggtcgtcgc cacccacgac gccactctcc gcgcctgacc ctcccgttgt
    13621 gcccgttctg ttcggcggat cacaacctga cggttgtgat ccgccgaaca gaacgggaag
    13681 gggtgcagaa gtatgcggcg gtgtgcatga gagtgcgcta gggtgctgcc atggcaggtg
    13741 acaccgcccg ggtcggcatc atcggagcgt cgggcttcac cggcgccgag ctgctgcgca
    13801 tctgcgccca gcacccggga ctggacgtcg ccttcgccac cggcgacagc caggccggca
    13861 ccgcggcagc cgacctgtac ccgggtctcg ccccggcgta tcccgacctg gtgttccagg
    13921 cgtgggatcc cgctctgctc gacggggtcg acggcgtgtt cgtcggactg ccccacggcg
    13981 cgagccagac gctgatggcc gacgtcctcg gcaaggtccg cacggtcgtc gacctggggg
    14041 ccgacttccg actctcggac ccggccgact accagcgctg gtaccacgaa ccccacaccg
    14101 cgcccgagct cctcgaccgg ttcgtctacg gcctgccgga gctgtaccgg gatcggatcg
    14161 agggtgccga cgcgatcgcg gctcccggct gctacccgac cgcgaccacc ctggcgctgc
    14221 gccccctggt cgacgccggg ttgatccaca ccgagggcat catcgtcgac gccgtctccg
    14281 gcgtgtccgg ggccggtcga cctcccaagc cgcacaccac gttctgcgcg gtcgacgaga
    14341 acgtcgaggc ctacggcctg ctggatcacc gccacaccgc cgagatggac atgaacctgg
    14401 gcgccaccgt cctgttcacc ccgcacctgg tcccgatgaa ccgcggcatc ctcgccacct
    14461 gctacgcgcg gcccgcggtc gacgggctca gcaccgacgc ggtgctcgac gtgctcgcgc
    14521 agcgctacgc ggacgagccg ttcgtcgtcg cgtcgacccg gtcgccgtcg accaaggcca
    14581 ccctcggcac caacgccgcc cacgtcaccg cccgctacga cgcccggacc gggaccgtcg
    14641 tgtccatcgc cgcgatcgac aacctgggca agggcgcgtc gggccaggcc gtgcagtgca
    14701 tgaacctggc gctcgggctg cccgagaccc agggactcgc cgtcgtgggg acggctccgt
    14761 gagccccgag gcgagcggcg ccccgagcgg ggggctcgat ccccgtcgcg accccgacct
    14821 ggccgtgcag gtcctgctgc aggctctgcc gtacatcgag gcgttccgcg gcgcggtcgt
    14881 cgtggtgaag ttcggcggca acgccatggg cgaccccgac ctgttcgagc agttcgcgtc
    14941 cgacatcgtg ctgatgcacc gcgtcgggat gcgcccggtc gtcgtgcacg gcggcggccc
    15001 gcagatcggc gagtggttgg acaagctcgg caaggccagc gagttcgtcg acggccgccg
    15061 cgtcaccgac gccgagaccc ttgacgtggc ccagatggtg ctgatcggca aggtgaacgc
    15121 cgacatcgtc accgccctca acggcttcgg tccgctggcg ttgggcctgg ccggcaccga
    15181 cgcgcatctg atcgaagccg aggcgcacga ccccgagctc ggcttcgtcg gagcggtcac
    15241 ccgggtcaat ccggcgttga tcacgcggac gctggccatg gacctcatcc cggtcatcgc
    15301 gaccatcggc gtcgaccgga ccggccagac ctacaacatc aacgccgatg acgccgccac
    15361 cgcgatcgcc gaggagctgg gcgcgcagaa gctcatcttc ctcaccgatg tccccggtct
    15421 gctcgccgat gccgaggacc cggcgtcgct catcacccgg gtcgacaccg acgaggtcgc
    15481 ccggctcatc gacgacggca ccatctccgg gggcatggtc cccaagatgt cgggctgcgc
    15541 ccgcgccgtc ggcggcgggg tcggatcggt gcacctgatc gacgggcgtc ggccgcacgt
    15601 gctgctgctc gagctgctca ccgacgccgg tgtcggcacg atggtcgtcc cggccgcggc
    15661 cgctgcggcg gacgaagggg agacctcgtg accgctccat cgccgctcat gtcgacctac
    15721 ccgccgtcgc cggtcacctt cgtgcgcggc gaggggtcct ggctctacga cgacaccggc
    15781 cgggcatacc tcgacctgct gtcgggtctg gcggtgacct cgctcgggca ctcgcatccg
    15841 gatgtggccg acgccctggc tgaacaggcc cgcacgttgc tgcacgtctc caacctgttc
    15901 ggcacggtcc ccggcccgca ggtcgccgcc accctcgacc ggctcctcgg cgggggtggc
    15961 caggtgttct tcgccaactc gggcgccgag gccaacgagt gcgccatcaa gctggcccgt
    16021 cgttgggccg gcgcgccggc cggccgctac aaggtcgtct cggctctcgg cagcttccac
    16081 ggtcggacgc tcgccacgct gcacgccacc ggccagcccg ccaagcacga gccgttccaa
    16141 ccgctgcccg acgggttcgt ccacgtcgca tgggacgacc tcgacgccct ggccgcggcg
    16201 atcgacccgt cggtcgcggc ggtgctgttg gagccggtcc agggtgaagg tggggtgaac
    16261 ccggccaccg tcgagtactt ccggggcgtc cgggacctgt gcgacgagcg cggcgtgctg
    16321 ttcatggtcg acgaggtgca gaccggcctg ggccgctgcg gcgcctggtt cgcgcatcag
    16381 cacttcggtg tggtgcccga cgtggtcacg atggccaagg cgttgggcaa cggcgtgccc
    16441 atcggggcct gctgggcccg cgccgatgtc gccgccgcct tcgagcccgg cgaccacgcc
    16501 accacctacg gcggtcaacc gctggcgacc gcggcggccc gagcggtcct ggcggtgatg
    16561 gaggaccggg acgtaccggc ccgggcccgt cacatcggtg aggtcgtcgc gtcggagctg
    16621 accgctgcac ccggggtgct cgacgtccgg ggcctcggcg ccctgatcgc cgtcgaggtg
    16681 gccgtcgacg cccggcagct gtgctcggag ctgctcggcc ggggcgtggt cgtcaacgcc
    16741 gtcacgccga cggccctgcg ccttgccccc agcctgctga tcgacgacgc cgagctccgc
    16801 tcgggcacct cgtcgatcgc cgagaccgtg gccgagatcc tggaaggtga cgagaactga
    16861 tgagtgcgaa cccgccgctg cggcacctgc tcgaggtcga cgatctgagc gcggtcgagc
    16921 tcgcccgcat cctcgatctg gccgagctga ccgatccgcc gccggtcctg gccggcagcg
    16981 gcggcgtgct gctgttcgag aagccctcgg cgcgcacccg cacctcgatg gagatggcga
    17041 tcgaccagct cggcggtcat ccggtcagcc tgtcagggtc cgaggtcggc atcgacaccc
    17101 gcgagaccgc cgaggacctg ggccgtctca tgtcgggcta cggccgtgtc atcggcgccc
    17161 gggtgttcga gcaccacaag ctcgagcgca tggcgtcggt ggcgtcggtg ccggtggtga
    17221 acctgctctc cgacgacgcc cacccggtgc agaccctggc cgacctgctg acgttgcgcc
    17281 agtgcttcgg ccgactcgac gggttgaccg tcacctatgt cggcgacgcc aacaacgtcg
    17341 cccgctcgct cgccatcggc tgcggcctgg tcggcgcggc gttccggatc tcgagccctc
    17401 ccggctaccg cttcgacgac acgtcgaccg accggatccg ctcggcgggc accgacctca
    17461 ccgtcgtcga cgaaccgacc gaggccgcct ccggcgcgga tgcgatctac accgacgcct
    17521 ggtactcgat gggtcaggag gaggagaagc ggatccgcca ggaggccttc gcccggtggc
    17581 gcgtcgacga gtccctgatg gacgcggcgg gtgacggcgc ggtgttcctg cactgcctgc
    17641 cggcgcaccg cggtgacgag gcgaccgacg gcgtcctcga cggcccccgg agccggatct
    17701 ggccccaggc ccacaaccgg atgcactcgg cccgcggcct gctgctgtgg ctcgccgagc
    17761 tgagtgccga tcaggacccc gaacggggcg gaccttcctg atggccggaa agcagcagcg
    17821 tcagcaccgc atcggccggc tgctggtcga gcaccgcgtc accaaccagg cgcagctgac
    17881 cgagctgctc gcggccgacg gcgtcaccgt gacgcaggcc acggtcagcc gtgacctcga
    17941 ggagctcggg gcgatcaagg tccgggtccc cggcggcgac accgtgtacg ccatccccga
    18001 gctgccccgt gagcaggtca gcccccagga ccacctccgg cgggtcctcg gcgactggct
    18061 ggtcgaggtc gagcgctccg gtgacctggt cgtgctgcgc accccgcccg gctccgcgca
    18121 cgtcgtggcg tcggccatcg accgaaacgg gctcgacggg gtcgtcggca ccgttgccgg
    18181 tgacgacacg ctcctggtga tcgcccgtga gggccagggc gacccggtgg cccttcagct
    18241 gcaacagttg gccggactgg cctgatctgg aactggccga accggcctga acgcaaactg
    18301 gccggaccgg cctgatcgaa aggacgacag aacatggcga agcgggtggt gctggcctac
    18361 agcggcgggc tcgacacctc ggtggcggtg cggtggatga tcgagaacct cggggtcgag
    18421 gtcgtctgcc tgtcggccga cgtcggccag gagggcaccc tcgagggcaa ccgggagaag
    18481 gcgctcgggg ccggggcgat cgcgtacgag gagctcgacc ttcgccagga gttcgcggac
    18541 gacttcctgg ccccgctcat caaggccaac gcgctctacg aggaccagta cccgctggtg
    18601 tcggcgctgt ctcggccgct catcgccaag aagcaggtcg agatcgcccg caagtacggc
    18661 gcggacggcg tcgcacacgg ctgcaccggc aagggcaacg accaggtgcg cttcgaggtc
    18721 agctacatgg cgctcgcccc cgacctggag cagctcgccc cggtgcgcaa ctggggcttc
    18781 acccgcgagg actcgatcga gtacgccgcc acccacggca tcccggtcgg ggccaccaag
    18841 gagaagctct actcgatcga cgacaacctc ttcggtcggg cgatcgagtg cggcgagatg
    18901 gaggacccgt gggccgagcc gcccgcaggg gtctggaaga tgaccgagcg gaccgccacc
    18961 gaacccgccg agttcaccct cagcttcgag cgcggcgttc ccgtcgccct ggacggcgag
    19021 gcccggccgc tgttcgagat cgtcatcgcc ctcaaccacc tggtcggcag ctacggctgg
    19081 ggacgcctcg acatggtcga gaaccgtcgc gtcggcatca agagccgcga gacctacgag
    19141 gcccccggcg ccctggcgat catgctggcg caccgcgacc tcgagtcgct gaccgtcgag
    19201 cgtgacctcg gccgccagaa gatcgcgctg cagcaccgct acgccgagct ggtctacgac
    19261 ggactgtggt actcgccgct gcgcgacgcc ctcgacgcgt tcgtcgacgt ctcccagcag
    19321 cacgtcaccg gcgacgtccg gctccgcctc gagcccaacc ggtgctgggt cgtcggtcgg
    19381 cgggccccga agtcgctcta cgactacgga ctcgccacct acgacgcggc ggaccggttc
    19441 cgccacgcgg attccgaggg cttcgtgcgc atctggggtc tcggcgtgca gacctggtcc
    19501 gacgtccagg gcccgggttc gcagcgatga ccgaggacgg ctcgcccgcc gatcggggcc
    19561 agctctggca cggtcggttc gacgtcccgc cggccgaggc gctcgtcgag ttcaccgagt
    19621 cgctcagcta cgaccagcgc ctcctgcccg acgacgtcgc ctgctccaag gcgcacgtcc
    19681 gggggctgca ccgggccggc ctcatcgacg agggggagct ggccgccctc ctcggtggcc
    19741 tggactcggt cgagatggag taccggaccg gggtgctcga cttcgtcgcc tccgacgagg
    19801 acgtccacac cgccatcgag cgccgggtca ccgagctggt cggcccggtc ggcggcaaga
    19861 tccacaccgg tcggtcgcgc aacgaccagg tcgccaccgc gttccgccat cacgtggtgg
    19921 gccagatggc ggcgaccgcc gagctggttc tcggtctcgt cgacgtgctc gccgatcggg
    19981 ccgaccgatc cctgaccggc ggcgtcgacg gtgacccggt gcgcctgccg ggctacacgc
    20041 acctccagcg ggcccagccg gtgctgctgg cgcatcacct gatggcgcac gcctgggcgc
    20101 tgctgcgaga tgtcgaccgc ctggccgact gccggcgccg cacccacgtg tcgccgctcg
    20161 gcgcgggcgc gctggccggg tcgtcgctgc cgctcgatcc cgacgcgacc gcggccgacg
    20221 ccgggttccc ggcgcggttc gagaactcgc tggacgccac ctcggaccga gacttcgtcg
    20281 ccgagtcgct gttcgtgtgc acgacgatcg gcctgcacct gtcgcggatg ggcgaggaga
    20341 tcgtcctgtg gaccagcgac gagttcggct tcgccgtcct cgacgacacc tacgcgaccg
    20401 gctcgtcgat gatgccgcag aaaaagaacc ccgacgtcgc cgagttggct cggggcaagg
    20461 ccggtcgcct gatcggtgac ctgaccggct tcctcgccac catgaagtcg ttgccgctcg
    20521 cgtacaaccg cgacctccag gaggacaagg agccgctgtt cgacgcgctc gacacggttc
    20581 gcctggcact gctcgcgatg accgggctgt acgcgacgat caccttccga cccgagccga
    20641 tggcggcggc ggcggactcg ccgtatgcgg cggcgaccga cctggccgag ctgctggtca
    20701 ccgacggcat cccgttccga tcggcccacg gcatcgtcgg ggcgttggtg cgtcggtccc
    20761 tcgacgaggg catccccctg gtcgacctcg tcgccgccga accggagctg ggcgagcggg
    20821 gcgtggccct gctccggccc ggggcgggca cggccaaccg cgccagcccg ggcgggggag
    20881 gccccgagcc ggtcgcagcc cagcgcggtc gtctcg
//
LOCUS       DAOQOD010000039        20905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_873_length_20905_cov_10.455300, whole genome shotgun sequence.
ACCESSION   DAOQOD010000039 DAOQOD010000000
VERSION     DAOQOD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_20905_cov_10.455300"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLZ72_05825"
     CDS             <1..233
                     /locus_tag="PLZ72_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35850.1"
                     /translation="SATAVRALPLPSDTGAWKEGTDLIAGLGLDEEGRRNEILHRFGE
                     VMVRAYGPADRDLLAWWWPRATGRRAGGADGA"
     regulatory      262..383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            465..1688
                     /locus_tag="PLZ72_05830"
     CDS             465..1688
                     /locus_tag="PLZ72_05830"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNA35851.1"
                     /translation="MSPDTTDDTTEEIPATGAWRPGQPVASRRFLRVAGEMTRRGSDP
                     DGLEHSGTSRFLRLEGGGRLDVVDVAYETWGTLAGDGANAVLICHALTGDAHVAGSSG
                     PGQPTPGWWDALVGPGRAVDTDRWFVVCSNVLGGCQGSTGPSSVAPDGRPHGSRFPVV
                     TIRDMVRVQAMLADHLGIGRWRAVLGGSMGGMQALEWATMFPERVERLVLASTTAAAS
                     AQQIAWSHIGRKAVAADPRFRGGDYYDAEPGDGPHRGLAVARMCAQVTYRSDEVFTDR
                     FHRTTLGPMEFTLDPIFDVEGYLDYHGLKLARRFDANSYMRLNRAMDLHDVARGRGST
                     EAALSRITAPALVAAVRSDGLYPPIQQHVLADGLASVGGDVTWVDVDSPHGHDGFLIE
                     TPQLAPPVRDFLERP"
     gene            1685..2926
                     /locus_tag="PLZ72_05835"
     CDS             1685..2926
                     /locus_tag="PLZ72_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA35852.1"
                     /translation="MTATPPEPVGDSASRPAVAPETATVLAGRERNGAALAPTIVTSS
                     TFVTPSVAEGRRLATSTTSDSFYGRYGNPTVNDFADAIAVLEGAEAARAFSSGMGAIT
                     AVVLGLCSSGDHIVTQRQLYGGTQLLFQGACPRLGIDVTFVDGTDPDEWAAAVRPGKT
                     MLLFAETPANPRLDLVDLEALGAIAGPMKVVDSTFATPLGQRPLDFGIDLSLHSATKG
                     IGGHNDVSLGVVAGSRELLDWIWGFAVLQGANASPYDAAGGLRGLRTLGVRLRHQSAS
                     ASQLAAALEADPRVAEVRYPGLASHPQHDLARRQMRLSGGQLTFDLVGGAEAGERFVE
                     STRICRLAPSLGGPETLVTHPASSTHVGLLPDELAAAGIGPGTIRMSVGLEDPDDVLA
                     DVRAALDAIDVDGSSRADAPG"
     gene            2948..3730
                     /locus_tag="PLZ72_05840"
     CDS             2948..3730
                     /locus_tag="PLZ72_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNA35853.1"
                     /translation="MPELIEVEMFRAAAEAVVGRTVSRVDAPDSWFLKHGLSADVLGA
                     AVPGAEVLAATRRGKLLMMPMSSGAVLGLRFGMTGRLAVDGRMPIERLEFSSRRVDPA
                     WVRFELEFVEGGCLSIIDPRRLGGVELDPDVSTLGPDASTVTVDELRSALGRSAAPLK
                     AVLLDQHRLAGLGNLLVDEILWRSRLDPLRPARSLDPDEQRALARSIRSTVQVLTRRG
                     GSHTGDLQVARVPGAACPRCSAPLDRRTVGGRTTYSCPQEQT"
     assembly_gap    3865..3964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3965..4426
                     /locus_tag="PLZ72_05845"
     CDS             <3965..4426
                     /locus_tag="PLZ72_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35854.1"
                     /translation="PALEARATESEQATTRLNRIVIALLVLAAVIAGATVYFWMRTRP
                     DRSRSTGRPERRGAVLVTADGHEEPLLDPGPAAGLRPPVGEDAWRVRPAPQLGGTPAQ
                     VGAGAAEAAPEPRPAQGSSTGWWASQEPDQAPTATGSGPRDRPEPGPDTAE"
     gene            4437..4655
                     /locus_tag="PLZ72_05850"
     CDS             4437..4655
                     /locus_tag="PLZ72_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35855.1"
                     /translation="MESFDPQDLVERFRERAAAVRRRQIPPVEGEARREFIRQAEVDF
                     RDFAIIGDADAVLDDGVLVLRVDLRPDR"
     gene            4770..5843
                     /locus_tag="PLZ72_05855"
     CDS             4770..5843
                     /locus_tag="PLZ72_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNA35856.1"
                     /translation="MTQVSDIGQQEGWVWACPYCRAPLASGSTQCSHCGSPLQDPDGD
                     DLFTTVAADYAEVIPPDPPFARDNMWTTDANDPDPYEVEDAVGESELVDDVGEDAAGA
                     GPDDVWDVPPFPGAEPGRAAAGFPGAGVGSPGFSAEPNRRPADVIEEPFSLLDDVVEP
                     VHHSGTLFSGRTNGRATADSTPSDRVGPFAAPAAPSAGPHTGAGATGPQLPPPFPDPT
                     PWSVPGGTAPPPPPVPDAHGLAAAISRLRPEAAEAAEVPISVCGALLVRDEVVLAAVT
                     GQMLGHAAVVVLTNQRVMVVNGRRWQPIVDVFALGPDLVVRGRHDRDVASLTFSDATQ
                     LSTVDAIGEVALAVELAERIRGN"
     gene            5909..5984
                     /locus_tag="PLZ72_05860"
     tRNA            5909..5984
                     /locus_tag="PLZ72_05860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5942..5944,aa:Gly,seq:gcc)
     assembly_gap    6046..6145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6146..7786
                     /locus_tag="PLZ72_05865"
     CDS             <6146..7786
                     /locus_tag="PLZ72_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNA35857.1"
                     /translation="ERIGLLVIDEAHCISDWGHDFRPDYRRIADVLTSLPPDVPVLAA
                     TATANDRVTHDVAEQIGARAHTFRGSLDRPSLHLGVVSLASSGERLAWLAGWVRARSG
                     PGIVYALTVSEVERIASFLVACGLSAAAYSGATDAAARAAIEDDLDQGRLDCVVATSA
                     LGMGYDNSRLAYVVHVGSPSSPVSYYQQVGRAGRGTSAAEVVLCPTPTDGDVWAYFDS
                     TAMPPRHVVDEVLTVLQRGGPASIADIEAGVNLRRGRLEVLLKILDVEGAVDRDGTRW
                     FRTEQPWTYDDERHHRLAAARRSEQQAMRSYVATSGCRLRYLRDALDDDGAENCGRCD
                     NCTGTPVDDGVDPDIQAAAQQHLRSAVVRLEPRKQWPRGLDGRSGRIGGDDRHEEGRA
                     LGFGSDPGWAEVLGELFRAPDAEPDDVLLRGVAAALKAWDWGRRPTWVAAVPSRRRPL
                     LVGGLARRLSEIGHLELVDAVRRVRTDAPPQEQMENSLNQAANVLDAFEFAPDLPLGP
                     GLVVDDTWRSGWTMTVVAEGLRRSGAGLVLPFVLWRRP"
     gene            8121..8513
                     /locus_tag="PLZ72_05870"
     CDS             8121..8513
                     /locus_tag="PLZ72_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35858.1"
                     /translation="MVAALATGAASLLAWRDVGGFSGGSETGWTEFDGGLGRGWIAVL
                     AGVLLALAGVLVAAEKGRAGRTLGTLTGVGLVVFSVLEWGLGLRGVRSGPGPGLWLLF
                     VVGLIVVAAIGILAPEPEPAPGHQAPAR"
     gene            8565..8641
                     /locus_tag="PLZ72_05875"
     tRNA            8565..8641
                     /locus_tag="PLZ72_05875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8599..8601,aa:Val,seq:gac)
     gene            complement(8823..>9307)
                     /locus_tag="PLZ72_05880"
     CDS             complement(8823..>9307)
                     /locus_tag="PLZ72_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA35859.1"
                     /translation="SSSLVSTDGGAARWASGRPLPTSGASGPPRDRGRRVRPRERLDD
                     GPDGTRARNRLTNASDLCAPDLIDVETTAVLRKRWIAGDLTDRRFSEAISDLEDLDLT
                     RYPTLSLMHRAFDLRSNVTAYDAAYIALAERLGCFLLTADQKVAAAPTVACAVEVLHP
                     "
     assembly_gap    9308..9407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9408..9599)
                     /locus_tag="PLZ72_05885"
     CDS             complement(<9408..9599)
                     /locus_tag="PLZ72_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35860.1"
                     /translation="MPNILVRDLPDDVHGALQRRAEQQGQSLQQYLTNELIRLVERPT
                     PEELFARVDRRRGGKVGLGQ"
     gene            9739..9960
                     /locus_tag="PLZ72_05890"
     CDS             9739..9960
                     /locus_tag="PLZ72_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNA35861.1"
                     /translation="MTDILIRDVPDDVLSAIDARAKRVGLSRSEYLRRALDRERLSDA
                     GPVTVEQLRRVAALVTDLNDPSVMDGAWS"
     gene            9957..10364
                     /locus_tag="PLZ72_05895"
     CDS             9957..10364
                     /locus_tag="PLZ72_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNA35862.1"
                     /translation="MRSWLIDKSAISRLHLDPRAGEWAERIERGLVRICTVTLLEVGY
                     SARSATDHRSLLDESPIASMPIEHLTPKIEDRALELQRVLSERGHHRAPSVPDLLIAA
                     TAELTDLSVLHVDKDFELIASVTGQRVERLDVD"
     gene            complement(10435..10950)
                     /locus_tag="PLZ72_05900"
     CDS             complement(10435..10950)
                     /locus_tag="PLZ72_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNA35863.1"
                     /translation="MSPADGDEVPWLDDREQAAWRSYLDLRDELALRLERHLQTASGL
                     SSADYQVLVHLSETDGDRARPVELCRTLRWQQSRLSHQLTRMERRGLVERLECHEDGR
                     GSIVALTPAGRSAIESAAPGHVRDLRDLLIEPLGRDGFLQLGDLARQVLDAMPEESTG
                     TTRADAGNRSS"
     gene            complement(10947..13340)
                     /locus_tag="PLZ72_05905"
     CDS             complement(10947..13340)
                     /locus_tag="PLZ72_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNA35864.1"
                     /translation="MRGSRWRASVAVLAVVVLAAACTQDVDGDDSSAGTGGLPADVGA
                     ATFSTTGSVGQVHVSGATADQELALYDRDGKTVGTGTTDAQGALIFREVDPGQGYRVA
                     TTDDPVLASTAVRVESPTSSLPEQSFYDGQHLEPGFTYITTRDGTTLSSSVYLPGPVE
                     DGPYPTVVEYSGYDPSRPGTNLLEENAEALRPIVGDDPSALCPVAPFACNAPAQPSSM
                     MALAMGYAVVAVNVRGTGCSGGAYDFFEPLQLTDGYDVIETVAAQPWVKDHHVGMVGL
                     SYPGIAQLFVASTNPPSLAAIAPLSVIDDTVRGTLSPGGIFNVGFAGSWAEDVGKKAE
                     AYGQGWEKARVDAGDTTCADNQLFRGQNVDVAAKARSQRYYPPKMADPLNASLFVDKI
                     DVPVFLTGSFQDEQTGGRFPLLFDRFTGSPVTHFTAMNGAHADGYAPVNLAEWKTFLD
                     LYVAGKLTDIPTPVKLFAPVIMDRIFKADIALPPLRFLDSPSIEQARAAYEAEAPINV
                     LFESGAGDTTQPGAPVPSVQVRTTEWPPPGTRPEAFYLGPDGTMSTTAPTGASKGSGA
                     DTASRFEVDPALAERTTFTGDSGDIFHALPDYDWQQEPDGSAAVFVSEPFDEDQVFAG
                     SASADLWIRSSSTSADLGVTLSEVRPDGKETFIQAGVLRAEHRKVAKGSTKLLPLHSL
                     LKSDAEPLEANTWTEARVEVFPFAHIVRAGSRIRLSVHTPGGDRPRWSYVIDEQPDGA
                     TIDVGHSAEHPSRLVMPLSPELLTDTPYPAALPPCPGLRGQPCRTFVPYANTETDDG"
     gene            13479..14420
                     /locus_tag="PLZ72_05910"
     CDS             13479..14420
                     /locus_tag="PLZ72_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35865.1"
                     /translation="MTLSVPGIDPDDPAFVYDEMAYFEENCAEYDLDAAADVVVERVE
                     RRLGDGRVVSALRWGSEPPRVVLVHGGAQNAHTWDTVALALRPHGVLAVDLPGHGRSS
                     WRADQAYDPVSNAEALAEALPPLIEGPALLVGMSLGGLTAIALAARLPEVARSVLVDI
                     TPGVDADKAAAVHAFIAGPQSFPTFGEIFERTVEFNPTRTPESLRRGILHNAHRCEDG
                     SWEWNYDRRPLAEHPEGDAAGVVAGLWEAISESTDPLVLVRGSNSPVVDEADVAELRR
                     RRPDARVVVVDDAGHSVQGDRPLELAALLRDEVAALA"
     gene            14536..17106
                     /locus_tag="PLZ72_05915"
     CDS             14536..17106
                     /locus_tag="PLZ72_05915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA35866.1"
                     /translation="MERAVPDSWEADVVLRDGHTVRMRSIRPDDAGLITRFHERQSPE
                     SIYFRFFSPRPRLSERELEHFTRVDHRDRVAFVALLDDELVGVARYERYEGTDTAEVA
                     FFVDDRHHGRGLATVMLEYLAAAARENGITRFRATTLPNNRKMLKVFASAGYEVASQL
                     DDGIIDVAFDLRPTDEVTAAVDRRERLAEAASVRRMLEPATVALIDAGAGGPRSTTGP
                     AHRPVPIEDACLVRLAERLRAGAFPGSVTLGGPAALDGLPEGVDLVVVAGPAAALPEV
                     LDRCADRAAGAVVVLSEADASQTEAILEVVRRRGLRLLGPGSLGVCNTDPAVALDALC
                     VEARPLPGTIGVLSESGEVVSSVLDHARRVGLGISTLVSTAEPADLNVADLLSYWADD
                     DRTRAVLLHLGSAPLPSRFVRAARAASRARTVAALASTARVAGGRPSPEQRRDRAMLR
                     QSGVIPVASLQELFAIGRLLADQPPPGGRGVAVVGASRAAVDLAVDACAAAGLDPVTG
                     HVGPAPDSLAAAASEPTVRSVVVIDTTPGPLPPPDLVDAVLGFSRDHSELTVVISAVG
                     SERPARLVDDATGVAVPLFTFPEHAGNALGRLATAREWRSTARVYGDETPSGLDVDAT
                     RALVERWRADGGPDQVLELDASRQEQLLAACGLRVADRREVRTVEQAVAAAEEIGWPV
                     ALKARRRDRRKRTALSGVALDVAGVDDLSATWARMEEALGPEGMQPVVVQRMVEQGLD
                     VAVRIRRPDRIGTVEVGLGGPMAAFDPWELGVLPLALPDASMLVAASSVARALTDPLN
                     RVPVVSLVHRLAALVDAVEEIHEVRADPVIVAGPVAWITDVRILIGSPRGELPVRHLG
                     "
     gene            complement(17083..18219)
                     /locus_tag="PLZ72_05920"
     CDS             complement(17083..18219)
                     /locus_tag="PLZ72_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35867.1"
                     /translation="MDFRWPDPIAELSAEAHRVGRAAAASRAVREDSWLIGTSREFSL
                     ELAERGWLGMTWPVEEGGGGRNALERFAVFEALISEGAPLATSWFADRQIGPTLLQFG
                     TPEQRARWLPEIIAGRSAWSIGMSEPDAGSDVASLRTPAVRRGDRWVVNGQKIWTSGA
                     ADADWCYLIARTDPDAPAHAGLSELVVDLHSPGIDIRPIVDATGNAHFCEVHLDGVEV
                     PTENLVGAENGSFKQVMRQMEHERGGIDRLVSNRRLYLDVVGAVHHDDPLVRQEVAAL
                     ESGYRIGRLMVLREVLGQAPPGFSAVTKTFCTEFEQRVADLCARVAGADALLWDRVAR
                     NVCYAPAYTIMGGTTSILRNIVGERVLGLPREPRPERSTQVADR"
     gene            complement(18272..19537)
                     /locus_tag="PLZ72_05925"
     CDS             complement(18272..19537)
                     /locus_tag="PLZ72_05925"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNA35868.1"
                     /translation="MADSITITDNRTGESLEVPILDGGIDAKAWSKLLPGIWFHDPGF
                     GSTSGAASSITELDGEAGFLRYRGYPIEQLAESSSYLEVAYLLIHGELPTPEQFENWR
                     YEITHHTFIHENVRKRFMEGFHHDAHPMGMLVSTIAALSTFYPEARELTPDVMMKQVV
                     RLIAKMPTLAAGAFRHSVGMPFVYPDNSLDFAANFLSMMWKTAEPRYDANPVLARALD
                     VLFILHADHEQNCSTTAMRTVGSAHADPYVATAAATAALYGPRHGGANEAVIRMLTEI
                     GDIEHVPAYVERVKAGDIRLQGFGHRVYKNYDPRARIIKKTADEVFEVTGKNPLLDIA
                     LKLEEVALSDPYFTDRRLYPNVDFYSGLIYQAMGFPMDMFTVLFAIPRTAGWLSHYLE
                     LLDQDSRIYRPRQLYVGHPVRDYTPIERR"
     gene            complement(19665..20588)
                     /locus_tag="PLZ72_05930"
     CDS             complement(19665..20588)
                     /locus_tag="PLZ72_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNA35869.1"
                     /translation="MDRKQLRAFVAVAEQRSFSAGARVLGTVQSNVSAHVARLEKQLG
                     VLLIDRSTNELTDEGRVVFERARRIEGEFEALDADVASLRDVVSGSVRLGAIGTTARW
                     LVAPLLDSVTAQYPEIRVIVLDATTTSLVPNLASGAVDLAIVNLPVDETDLLVEPLFD
                     EDRILIVPDGHPLADHDSVTLSELSRHELLLEARGTAFRDVLDQAATAEGVVLRPKAE
                     LDGMRLLASLVFAGFGAGVVPASAIPPNVEATWRRIPVEGVPGRSVGLARRRRGLPTA
                     AQRVVAEQIRRTIAEGAASVPGVYPTLRPAT"
BASE COUNT         2745 a         7231 c         7560 g         3069 t
ORIGIN      
        1 tgtcggcaac cgcggtgcgg gcgctgcccc tgccgagcga caccggagcg tggaaggagg
       61 gaaccgacct gatcgccggc ctgggcctcg acgaggaggg ccgccgcaat gagatcctgc
      121 accgcttcgg cgaggtgatg gtccgggcgt acgggccagc ggaccgggac ctgctcgcct
      181 ggtggtggcc gcgagcgacg ggccgtcggg ccggcggcgc cgatggtgct tgaccggggt
      241 cacccggagc gtgttctctg gtgcgatcat caggagaggc cccgtcgggg gtccggcaac
      301 ctgggacgga cacccaaggt gcctttccgc ttcggcgggg atgtggccga ccggccgagc
      361 cggtggcaac caagtgtccg aagggccgcc gcgtgcggag atccagggtc gccggtcgcc
      421 ggcgcgggcg tcgtcccgcc ccgaccctcc gaccccaccg cgccgtgagc cccgacacca
      481 ccgacgacac caccgaggag atccccgcca ccggggcctg gcgacccggc caacccgtcg
      541 cctcgcgtcg gttcctgcgg gtggcgggtg agatgacccg acgcgggtcc gatcccgacg
      601 gcctggagca cagcggcacg agccggttcc tccggctgga gggcggcggg cgcctcgacg
      661 tggtcgacgt cgcctacgag acgtggggca cgctcgccgg cgacggtgcg aacgcggtgc
      721 tgatctgcca cgccctcacc ggcgacgccc acgtggccgg atcgtcgggg ccgggccagc
      781 ccaccccggg ctggtgggac gccctggtcg gcccgggtcg tgcggtcgac accgaccgct
      841 ggttcgtcgt gtgctccaac gtgctcggag gctgccaggg aagcaccgga ccgtcgtcgg
      901 tcgcgcccga cggccggccg cacgggtcgc ggttcccggt cgtcaccatc cgcgacatgg
      961 tgcgagtcca ggccatgctg gccgaccacc tcgggatcgg gcggtggaga gcggtcctcg
     1021 gcggctcgat gggaggcatg caggcgctcg agtgggcgac catgttcccg gagcgggtcg
     1081 agcgtctggt cctggcctcg accacggccg cggcgtcggc tcaacagatc gcgtggagcc
     1141 acatcgggcg caaggctgtc gccgcggacc cccggttccg gggcggcgac tactacgacg
     1201 ccgagcccgg cgacggaccg caccgaggcc tggccgtcgc gcggatgtgc gcccaggtca
     1261 cctaccgcag cgacgaggtg ttcaccgatc gcttccaccg caccaccctc ggtccgatgg
     1321 agttcaccct ggatccgatc ttcgacgtcg agggctacct cgactaccac ggcctcaagc
     1381 tcgcccgccg gttcgacgcc aacagctaca tgcgactgaa tcgggcgatg gacctccacg
     1441 acgtggcccg cggccggggc agcaccgaag ccgccctgag ccggatcacc gcccccgcgc
     1501 tggtcgccgc ggtgcgctcc gacggcctct acccgccgat ccagcagcac gtgctcgccg
     1561 acgggttggc gtcggtcggc ggcgacgtga cgtgggtcga cgtcgactcg ccccacggcc
     1621 acgacggctt cctgatcgag accccgcagc tcgccccgcc cgtccgcgac ttcctggaga
     1681 gaccatgacc gcaacccctc ccgagcccgt cggcgactcc gcgtcccggc cagcggtcgc
     1741 acccgagacg gcgacggtgc tcgccggccg ggagcgcaac ggcgccgcgc tggcgccgac
     1801 gatcgtgacc tcgtcgacgt tcgtgacccc gtcggtggcc gagggccgtc gcctggcgac
     1861 gtcgaccacc tcggactcgt tctacggccg ctacggcaac ccgaccgtca acgacttcgc
     1921 cgacgcgatc gcggtcctgg agggcgccga ggcggcccgt gcgttctcgt cggggatggg
     1981 cgcgatcacc gcggtggtgc tcgggctgtg ctcgagcggc gaccacatcg tcacccagcg
     2041 ccagctctac ggtgggaccc agctgttgtt ccagggggcc tgcccccggc tcggcatcga
     2101 cgtcaccttc gtcgacggga ccgaccccga cgagtgggcc gccgcggtcc gacccggcaa
     2161 gacgatgctc ctgttcgccg agacgccggc caacccccgc ctcgacctgg tcgacctcga
     2221 ggcgttgggc gccatcgccg gtccgatgaa ggtggtcgac tccaccttcg ccaccccgct
     2281 cggccagcga ccgctggact tcggcatcga cctgtcgctg cactcggcca ccaaggggat
     2341 cggcggccac aacgacgtca gcctgggcgt ggtggcgggc agccgtgagc tcctcgactg
     2401 gatctggggg ttcgcggtgc tgcagggcgc caacgcctct ccgtacgacg ccgcaggcgg
     2461 gttgcgcggc ctgcgcaccc tcggcgtgcg gctgcgccac cagtcggctt ctgcgtcgca
     2521 gctcgccgcg gcgctggagg ccgacccccg ggtcgccgag gtgcgctacc cgggcctggc
     2581 ctcacacccc cagcacgacc tcgcccgccg ccagatgcgg ctgagcggcg gccagctcac
     2641 cttcgacctg gtcggtggcg ccgaggcggg ggagcgcttc gtcgagtcga cccggatctg
     2701 ccgtctggcc ccgtcgctcg gcggccctga gaccctggtg acgcaccccg cgtcgagcac
     2761 ccacgtcgga ctgctgcccg acgagcttgc cgccgcgggg atcgggccgg ggacgatccg
     2821 catgtcggtg gggctcgagg accccgacga cgtcctggcc gatgtgcgcg ccgccctcga
     2881 cgccatcgat gtcgacggat cgtcccgtgc ggacgcgccc ggctgacggc acacgacggg
     2941 aaccaccgtg ccggagctga tcgaggtcga gatgttccgc gccgcggccg aagcggtcgt
     3001 ggggcggacc gtgagccggg tcgacgcccc ggacagctgg ttcctcaagc acggtctgtc
     3061 cgccgacgtg ctcggcgccg cggtgccggg agccgaggtc ctggccgcga cccgccgcgg
     3121 gaagctgctg atgatgccga tgagcagcgg ggccgtcctc gggttgcggt tcgggatgac
     3181 cggccggctc gcggtcgacg ggcggatgcc gatcgaacgc ctcgagttct cgagtcgacg
     3241 tgtggatccg gcgtgggtcc gcttcgaact ggagttcgtc gagggcggat gcctgtcgat
     3301 catcgaccca cgacgtctcg gcggggtgga gctcgacccc gacgtctcga cgctcggccc
     3361 cgacgcatcc acggtgaccg tcgacgagct gcgctcagcc ctcgggcggt cggcggcgcc
     3421 gctgaaggcg gtcctgctcg accagcaccg cctggccggt ctcgggaacc tgctggtcga
     3481 cgagatcctc tggcgctccc gtctggaccc gctgaggccg gcccgttcgc tcgatcccga
     3541 cgagcagcgc gccctggccc ggtccatccg ttccaccgtg caggtgctga ctcgccgagg
     3601 gggctcccac accggcgatc tccaggtggc ccgggtgccc ggcgccgcgt gcccacgctg
     3661 ctcggccccg ctcgaccggc gcaccgtcgg aggccgtacc acctactcct gcccgcagga
     3721 gcagacgtga ggcccgaacc tgtcccgatc ccggtcaccg gcgccccgcg acgacgccac
     3781 tagggtttcg cggcatgtgg gccagcccgg actcccgacg cctcccggcc cggtgacccg
     3841 cgccgccgtg gccgtggtgc ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnncccgcc ctcgaggcac gtgccaccga gagcgaacag gccaccaccc ggctcaaccg
     4021 catcgtcatc gccctgctgg tgctggccgc ggtgatcgcg ggggcaaccg tgtacttctg
     4081 gatgcgcacc cgaccggacc gcagccgatc caccggtcgg ccggagcgtc gaggtgccgt
     4141 tctggtcacc gcggacggcc acgaggagcc cctgctcgac ccgggtcccg ccgccggatt
     4201 gcgccccccg gtcggcgagg acgcctggcg ggtccggccc gcgccgcagc tcggcggaac
     4261 acccgcgcag gtgggcgcag gggccgccga ggcagcaccg gagccccgcc cggcacaggg
     4321 gtcttcgacg ggttggtggg cgtcgcagga gccggatcag gcgccgaccg cgaccgggtc
     4381 gggcccacgg gatcgcccgg agccgggccc ggacacggcc gagtagcctc ggcccgatgg
     4441 agtccttcga tccccaggac ctggtcgagc gcttccggga acgggccgcg gcagtccgtc
     4501 ggcgtcagat cccaccggtc gagggcgagg cccgacgcga gttcatccgt caggccgagg
     4561 tcgacttccg ggacttcgcg atcatcggcg acgccgacgc cgtcctcgac gacggcgtcc
     4621 tggtgcttcg ggtcgatctg cgtcccgacc gctgacccgc gttgccgatc gcgggcgacc
     4681 cgaccgctcg cgtccggaga ggacccgagg gaggcttgtc gccctcgatc ccgtacgctg
     4741 gatggcgttc agccacaccg gaggagcggg tgacgcaggt gtccgacatc ggccagcagg
     4801 aggggtgggt ttgggcctgc ccgtattgcc gtgccccgct ggcgtccgga tcgacgcagt
     4861 gctcgcattg cggctctcca ctccaggacc ccgacggtga cgacctgttc accacggtgg
     4921 cggcggacta cgccgaggtg atcccgcccg acccgccctt cgcccgggac aacatgtgga
     4981 ccaccgacgc caacgacccg gacccctacg aggtcgagga cgcggtgggg gagtccgagc
     5041 tggtcgacga tgtcggcgag gacgcagcgg gggccggtcc cgacgacgtg tgggacgtac
     5101 cgccgttccc cggcgcggag cccgggaggg cggccgctgg attcccggga gcgggagtgg
     5161 gttcgccggg tttcagcgcg gagccgaacc gtcgtccggc cgacgtcatc gaggagccgt
     5221 tctccctgct cgacgacgtg gtcgaaccgg tgcaccacag cgggacgctg ttctcgggtc
     5281 ggaccaacgg ccgagccacc gcggacagca cgccgtccga tcgggtgggc ccgttcgcag
     5341 ctccggccgc gccttccgcc gggccccaca ccggggctgg ggccaccggt ccgcagctgc
     5401 cgccgccgtt ccccgatccc actccgtggt ccgtccccgg gggcacagca cctccaccgc
     5461 cgcccgtccc cgatgcccac ggcctggccg cggcgatatc ccgactgcga cctgaggcgg
     5521 ccgaggctgc cgaggtcccg atctcggtgt gcggggccct gctcgtccgc gacgaggtgg
     5581 tgctggcggc tgtcaccggt cagatgctcg gccacgccgc ggtggtggtc ctgaccaacc
     5641 agcgcgtcat ggtggtgaac ggacgccgct ggcagccgat cgtcgacgtg ttcgcgctcg
     5701 gtccggacct cgtggtccgc ggtcgccacg accgcgacgt ggcgtcgctc accttctcgg
     5761 acgccaccca gctctccacc gtcgacgcga tcggcgaggt cgccctggcc gtcgagctcg
     5821 ccgagcggat ccgcggcaac tgagggagtc gacccgggga gcggccaact tgtgagcccg
     5881 acggcctctg ctacagtcgg ccgtcctcgc ggacgtagct cagttggtag agcgcaacct
     5941 tgccaaggtt gaggtcgccg gttcgaggcc ggtcgtccgc tccagaggag gagcccccgc
     6001 cccggcgggg gctcctccgc gcacgggccc gggtcgaccg tcgatnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnngagcg gatcgggctc ctggtgatcg acgaggcgca
     6181 ctgcatctcc gactggggtc acgacttccg gcccgactac cgccgcatcg ccgacgtcct
     6241 cacctcgctg ccccctgatg tcccggtgct cgccgccacc gccacggcca acgaccgggt
     6301 gacccacgac gtggccgagc agatcggagc ccgggcccac acgttccggg gctcgctcga
     6361 ccggccgtcg ctgcatctcg gcgtggtgtc gttggccagt tcgggtgagc gcctggcctg
     6421 gctggcgggc tgggtccggg cccggagcgg cccgggcatc gtgtacgcgc tgaccgtctc
     6481 ggaggtcgag cggatcgcgt cgttcctggt cgcctgcggc ctgtcagcgg ccgcctactc
     6541 aggggccacc gacgccgcag cccgcgccgc catcgaggac gacctggacc agggtcgcct
     6601 cgactgcgtc gtcgccacgt cggcgctcgg gatgggctac gacaactccc ggctggccta
     6661 cgtcgtgcac gtcggctctc cgtcgtcgcc ggtgtcgtac taccagcagg tcggccgggc
     6721 gggtcggggg accagcgcgg ccgaagtcgt cctctgcccg acgccgaccg acggagacgt
     6781 gtgggcctac ttcgactcca ccgccatgcc gccccgccac gtggtcgacg aagtgctgac
     6841 ggtcctgcag cgcggagggc cggcctcgat cgcggacatc gaggccgggg tgaacctgcg
     6901 gcggggccgg ctcgaggtgc tgttgaagat cctcgacgtc gagggtgccg tcgaccgcga
     6961 cggcacccgc tggttccgca ccgagcagcc ctggacctac gacgacgagc ggcaccatcg
     7021 gctggcagcg gcgcgtcgct ccgaacagca ggccatgcgg tcctacgtgg cgacctccgg
     7081 ctgccggctg cgctacctgc gcgacgcgct cgacgacgac ggcgccgaga actgcggtcg
     7141 atgcgacaac tgcacgggga cccccgtcga cgacggagtc gaccccgaca tccaggctgc
     7201 agcgcagcag cacctccgta gcgcggtggt gcgcctggag ccccgcaagc agtggccgcg
     7261 gggcctggac ggacggtcgg ggcgcatcgg cggggatgac cgccacgagg agggtagagc
     7321 cctcgggttc gggtcggatc ccggctgggc cgaggtgctc ggggagctgt tccgtgcgcc
     7381 cgatgccgaa cccgacgacg tcctgctccg gggagtggca gccgccctga aggcgtggga
     7441 ctggggtcga cggcccacgt gggttgccgc ggtacccagt cggcgccggc cgttgctcgt
     7501 cgggggcctg gcccgtcggc tgtcggagat cggtcatctg gagctcgtcg acgccgtgcg
     7561 acgcgtccgc accgacgccc cgccgcagga gcagatggag aactcgctca accaggcggc
     7621 caacgtgctc gacgccttcg agttcgcgcc ggacctcccg ctcggacccg ggttggtggt
     7681 ggacgacacc tggcgatccg gctggaccat gaccgtcgtc gccgaggggc tgcgccgctc
     7741 cggcgccggg ctggtgctgc ccttcgtcct gtggcgccgc ccgtgagctc tccggtcgtg
     7801 gcgtcgggcg ccgggtcagg tcacagcgac ggaacgcctg cgtcgatcct cagcgcgggg
     7861 gaccggtccg ctgtccgggc tggcaagctg gagcggtgag ttctcagctc ccgcccccgg
     7921 gtccagcccg cagtgccgtc tccgacgcgg accatcagtg ggccgctccg tcggcggctt
     7981 ccacgccgtc ggttccacca tcgcccgcga cgccttcctc gggctgggag gccggtgccc
     8041 cgctcggcgg gatcgccccc gcgccggcag cgcctgctcc tccggcgtca cgccgggacc
     8101 tgcttctcac ctcgatcctg ttggtcgccg ccctcgccac cggtgcggcc agccttctgg
     8161 cctggcgtga cgtcgggggg ttcagcggcg ggtccgagac cggctggacg gagttcgacg
     8221 gcgggctcgg acggggatgg atcgccgtgc tcgccggtgt gctcctggcg ctcgccggtg
     8281 tcctcgtggc cgccgagaag ggccgggcgg ggcgcaccct cggcacgttg accggagtag
     8341 ggctcgtggt gttctcggtg ctcgagtggg gtctgggtct gcgcggggtg cgcagcgggc
     8401 cgggcccggg tctctggctg ctgttcgtgg tcggactgat cgtggtggcg gccatcggca
     8461 tcctggcccc cgaaccagaa cccgcgcccg gccatcaggc accggctcgg tgagcgtcgt
     8521 ggcgtcagac cggcggtccg ggcctgctat gttgtcgccc tcgcgggcgt atagctcagt
     8581 tggctagagc gcttcctcga cacggaagag gtccacggtt caagtccgtg tacgcccacc
     8641 agtcaggtcc cctgccacgg caggggatcc tgcgttttcg ggtccggcca ccgtggtccg
     8701 gcccaacggg gcggacctgg ctcctgatgt gcggcacgag caggctccga gtctcgcctg
     8761 acgacgacga acaggacacg cgccgcgaga tcgtcgcatc gccaacgtcc cggtcccggt
     8821 gttcagggat gcaggacctc gacagcgcag gcgaccgtgg gagcggcggc cactttctgg
     8881 tcggcggtga gcaggaagca gccgagtcgt tcggcgagag cgatgtatgc ggcgtcgtag
     8941 gcggtcacgt tcgatcgcaa gtcgaacgcc cggtgcatca gcgagagcgt gggatagcga
     9001 gtgaggtcga ggtcctcgag gtcgctgatg gcttcggaga agcgccggtc ggtcaggtca
     9061 ccagcgatcc accgtttgcg gaggaccgct gtggtctcca cgtcgatcag atccggcgca
     9121 cacaggtcgc tcgcgttggt gagacgattg cgtgcgcggg tgccgtcggg gccgtcatca
     9181 aggcgttcgc gaggacggac gcgtcgacca cgatcacgag gcgggccgct cgccccggag
     9241 gtcggcaacg gcctgcccga ggcccacctt gccgccccgc cgtcggtcga cacgagcgaa
     9301 gagctctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg cccgaggccc
     9421 accttgccgc cccgccgtcg gtcgacacga gcgaagagct cttcgggggt cgggcgctcg
     9481 accagacgga tgagctcgtt cgtcaggtac tgctggagcg actggccctg ctgctccgcc
     9541 cgacgttgga gtgcgccatg gacatcgtct gggaggtctc gcacgaggat gttcggcatg
     9601 cttgcaatat gcaagcatgc cgaaggcgat gcaaggctct cttgttacgc catgccccac
     9661 gagacgccgc ctcagagtcg gctggccggc ggcaagatat cgagcagcga tatcatctga
     9721 tatcggaggt ggtgcagaat gacggacatc ctgatcagag acgttcccga cgacgtcctc
     9781 agcgcgatcg atgccagggc gaagcgggtc gggctgtcgc gcagcgagta cctccgacgg
     9841 gcgctcgacc gcgagaggct cagtgatgca ggcccggtca ccgtcgagca gttgcggcga
     9901 gtcgcggcgc tcgtcaccga cctgaacgat ccttccgtga tggacggcgc ctggtcgtga
     9961 gatcgtggct catcgacaag tcggccatct cacgacttca cctcgaccct cgcgccggcg
    10021 agtgggcgga gcgcatcgag cggggcctcg tgcggatctg caccgtcacg ctgctggaag
    10081 tcggctactc cgcccgctcc gcgaccgacc accggtcgct gctggacgaa tctccgatcg
    10141 cgtcgatgcc gatcgagcac ctcacgccga agatcgaaga ccgcgcactc gaactccagc
    10201 gggtgctgtc tgagcgaggg caccacaggg caccctccgt gccggacctg ctgatcgcag
    10261 ccacagccga gctcacggac ctcagcgtcc tgcacgtgga caaggacttc gagttgatcg
    10321 cttccgtcac gggccaacgc gtcgagcggc tcgacgtgga ctgacctgga gcccgcgtac
    10381 tggcgtccgt gcccgttcgg cccgaacgtc gacgaatcgg atcggttcga ccgctcagct
    10441 cgaccggttc cccgcgtcgg cccgggtggt gccggtggac tcctcgggca tggcgtcgag
    10501 cacctgtcga gccaggtctc ccaactgcag gaagccgtcg cgcccgaggg gttcgatcag
    10561 caggtcccgc aggtcccgga cgtgtccggg cgcggcggac tcgatcgccg accggcccgc
    10621 cggggtcagg gccacgatcg atccccgacc gtcctcgtgg cactccagac gttccaccag
    10681 gccccgccgt tccatccggg tcagctggtg cgacaggcgg ctctgctgcc agcgcagggt
    10741 gcggcacagc tcgacgggcc gggcccgatc cccgtcggtc tccgacaggt gcaccaggac
    10801 ctggtagtcg gcgctggaga gccccgaggc ggtctgcagg tgccgttcca gacgcagggc
    10861 caactcgtcg cgcaggtcga ggtaggaccg ccacgcggcc tgctcccggt cgtccagcca
    10921 cgggacctcg tcgccgtccg ccgggctcat ccgtcgtcgg tctcggtgtt cgcgtagggg
    10981 acgaaggtcc gacacggctg tccgcgcagt cccgggcacg gcgggagggc ggcggggtac
    11041 ggcgtgtcgg tcaacagctc gggcgacagc ggcatcacca ggcgtgatgg gtgctcggcg
    11101 gagtggccga cgtcgatcgt ggcgccgtcg ggctgctcgt cgatcacgta gctccagcgg
    11161 ggacggtcgc cgccgggggt gtgcaccgag aggcggatcc gtgacccggc ccgcacgatg
    11221 tgggcgaacg ggaacacctc gacgcgggcc tcggtccacg tgttcgcctc gagcggttcg
    11281 gcgtcggact tcaggaggga gtgaaggggc agcagcttgg tcgacccctt ggccaccttg
    11341 cggtgctcgg cgcgcagcac cccggcctgg atgaaggtct ccttgccgtc cgggcggacc
    11401 tccgacagcg tcacccccag gtcggcgctg gtcgaggagg accggatcca caggtccgcg
    11461 ctggccgaac ccgcgaagac ctgatcctcg tcgaacggct ccgacacgaa caccgcggct
    11521 gaaccgtcgg gttcctgctg ccagtcgtag tcgggcaggg cgtggaagat gtcgccgctg
    11581 tcgccggtga aggtggtgcg ctcggccagg gccggatcga cctcgaagcg ggacgccgtg
    11641 tcggcaccgg agcccttgct cgccccggtc ggggcggtgg tgctcatggt cccgtcgggg
    11701 cccaggtaga aggcttcggg ccgggttccg ggcggcggcc actcggtggt gcggacctgg
    11761 accgacggaa ccggcgcccc cggttgggtg gtgtcgccgg cgcccgactc gaacaggacg
    11821 ttgatcgggg cttcggcttc gtaggccgcc cgggcctgct cgatcgaggg cgagtcgagg
    11881 aagcggagcg gggggagggc gatgtcggcc ttgaagatcc tgtccatgat caccggggcg
    11941 aagagcttga ccggcgtggg gatgtcggtg agcttcccgg cgacgtagag gtcgaggaac
    12001 gtcttccact cggcgaggtt gaccggcgcg tagccgtcgg cgtgggcacc gttcatggcg
    12061 gtgaagtgcg tgaccgggga tccggtgaag cggtcgaaca ggagcgggaa ccggccgccg
    12121 gtctgctcgt cctggaagga gccggtcagg aagaccggga cgtcgatctt gtcgacgaac
    12181 aacgacgcgt tgagtgggtc ggccatcttc ggcgggtagt agcgctggga acgggccttg
    12241 gcggcgacgt cgacgttctg gccacggaac agctggttgt cggcgcaggt cgtgtcgccg
    12301 gcgtcgacgc gggccttctc ccagccctgg ccgtaggcct cggccttctt gccgacgtcc
    12361 tcggcccacg atccggcgaa gccgacgttg aagatcccgc cgggggacag ggtgccccgg
    12421 acggtgtcgt cgatgaccga cagcggagcg atcgcggcca gcgacggcgg gttggtcgag
    12481 gcgacgaaca gctgggcgat tcccgggtag gagagcccga ccatgccgac gtggtggtcc
    12541 ttgacccagg gctgggccgc gacggtctcg atgacgtcgt agccgtcggt gagctggagc
    12601 ggctcgaaga agtcgtaggc cccgccggag caccccgtgc cccggacgtt caccgcgacg
    12661 acggcgtagc ccatggcgag cgccatcatc gacgagggtt gggccggtgc gttgcaggcg
    12721 aagggcgcga cggggcacag agcggacggg tcgtcgccga cgatcggtcg gagggcctcc
    12781 gcgttctcct cgagcaggtt ggtgcctggc cgtgacgggt catagcccga gtactcgacc
    12841 accgtcgggt agggcccgtc ctcgaccgga cccggcaggt acaccgacga cgacagggtc
    12901 gtgccgtccc gggtcgtgat gtaggtgaag cccggctcca ggtgctggcc gtcgtagaag
    12961 gactgctcgg gcagcgacga cgtcgggctc tcgacccgaa ccgccgtcga ggcgaggacc
    13021 ggatcgtcgg tggtcgccac ccggtacccc tgtccgggat cgacctcgcg gaagatcaac
    13081 gccccctgcg catcggtggt gccggtcccg acggtcttgc cgtcgcggtc gtacagggcc
    13141 agctcctgat cagcggtggc gccgctcacg tggacctggc cgacgctgcc ggtggtcgag
    13201 aacgtggccg ctccgacatc ggcgggcagg ccgcctgttc ctgcggagga gtcgtcgccg
    13261 tcgacgtcct gggtgcaggc ggctgcgagc acgacgaccg ccagcaccgc caccgacgcg
    13321 cgccagcgcg atcccctcat ccggtgcatc ctggcggtcc tccctcgtca cccccgtccc
    13381 gccggtcggg aggggcctcg gttcgacgtg catcttgtca tcccgacgcg ccgcgttcgc
    13441 ggcggaccct gcccccgggg gctcctacat tggcggccat gaccctctcg gtacccggga
    13501 tcgatcccga tgacccggcc ttcgtctacg acgagatggc ctacttcgag gagaactgcg
    13561 ccgagtacga cctggacgca gcggcggacg tcgtggtcga gcgggtggag cgacgcctcg
    13621 gcgacggtcg ggtggtgtcg gctctgcgct gggggtcgga acctccccgg gtggtcctcg
    13681 tccacggcgg cgcccagaac gcgcacacct gggacaccgt cgctctggct ctgcgcccgc
    13741 acggcgtcct cgccgtcgac ctgcccggac acggccgctc gagctggcga gccgaccagg
    13801 cctacgaccc ggtctccaac gccgaggccc tggccgaggc cctgccgccg ctgatcgagg
    13861 gtccggctct gctggtgggc atgtcgctcg gcgggctgac cgcgatcgcg ctcgcggcgc
    13921 gtctaccgga ggtcgcccgg tcggtgctcg tcgacatcac ccccggtgtc gacgccgaca
    13981 aggccgcggc ggtgcacgcg ttcatcgccg gtccgcagtc gtttccgacc ttcggtgaga
    14041 tcttcgagcg cacggtcgag ttcaacccga cccgcacccc ggagtcgttg cgtcggggca
    14101 tcctccacaa cgcccaccgt tgcgaggacg ggtcctggga gtggaactac gaccggcgtc
    14161 ccctcgccga gcaccccgag ggcgatgcgg ccggggttgt ggcgggtctg tgggaggcga
    14221 tctccgagtc gaccgatccc cttgtgctgg tgcgcggctc gaacagcccc gtggtcgacg
    14281 aggcggacgt cgccgagctg cggcgtcggc gtcccgacgc ccgtgtcgtc gtggtcgacg
    14341 acgccggtca cagcgtccag ggcgaccggc cgctcgagct ggcggcgctg ctgcgcgacg
    14401 aggtcgccgc gctcgcctga ccggccccac acccgctttg tcccgcacag gcgcggtgat
    14461 caccgcgcct gtgcgggaca aagcggaggg ggtcgccgag gcggtgcagc ggggccggtc
    14521 ggtagtgtcg ccgcggtgga gcgtgccgtg cccgactcgt gggaagccga cgtcgtcctc
    14581 cgcgacggcc acaccgtacg gatgcgttcg atccgacccg acgacgccgg gttgatcacc
    14641 cgcttccacg aacgccagtc cccggagtcg atctactttc ggttcttctc gccgcggcct
    14701 cggttgtcgg agcgcgagct cgagcacttc acccgcgtgg atcaccgcga tcgggtcgcg
    14761 ttcgtcgccc tgctcgacga cgagctggtc ggagtcgccc ggtacgagcg ctacgagggg
    14821 accgacaccg ccgaggtggc gttcttcgtc gacgaccggc accacggtcg ggggctggcg
    14881 accgtcatgc tcgagtacct ggccgcggcg gcccgcgaga acggcatcac ccggttccgg
    14941 gccacgaccc tgcccaacaa ccgcaagatg ctcaaggtct tcgcctccgc gggttacgag
    15001 gtcgccagcc agctcgacga cggcatcatc gacgtggcgt tcgacctacg tcccaccgac
    15061 gaggtgaccg cggcggtgga ccggcgtgaa cggttggccg aggccgcgtc ggtgcgacgg
    15121 atgctcgagc ccgcgaccgt ggcgctcatc gatgccggcg ccggcggtcc gaggtcgacc
    15181 accggtcccg cgcatcgccc ggtcccgatc gaggacgcct gcctggtccg actggccgag
    15241 cgactccgcg ccggtgcgtt ccccggatcg gtcaccctcg gcgggcccgc cgcgctcgac
    15301 gggttgcccg agggggtgga cctggtggtg gtcgcgggcc cggccgcggc gctccccgag
    15361 gtcctcgacc ggtgcgcgga tcgagccgcc ggcgcggtcg tggtgctgtc cgaggcggac
    15421 gcctcccaga ccgaggccat cctcgaggtc gtccgacgcc gtggtctgag gctcctcggg
    15481 cccggcagcc tcggagtctg caacaccgac cctgcggtcg ccctggacgc tctgtgcgtc
    15541 gaggctcgac cgttgccggg gacgatcggc gtgctgagcg agtcgggcga ggtggtgtcg
    15601 tcggtgctgg accacgcccg acgggtcggc ctcgggatct cgaccctggt gtcgaccgcg
    15661 gagcccgccg acctcaacgt ggccgacctc ctgagctact gggccgacga cgaccgcacc
    15721 cgggcggttc tcctccacct gggttcggca ccgctgccgt cacgcttcgt gcgggcggcc
    15781 cgtgcggcgt cccgggcgag gacggtcgcc gcgttggcat cgacggcgag ggtggccggc
    15841 ggtcgaccga gccccgagca gcgtcgggac cgggcgatgc tgcgccagtc gggggtcatc
    15901 ccggtggcga gcctccagga gctgttcgcc atcgggcgtc tcctcgcgga ccagccgccg
    15961 ccgggtggtc ggggcgtggc ggtggtcggc gcctctcgtg ctgcggtcga tctggccgtc
    16021 gatgcgtgtg cggccgccgg cctggacccg gtcaccggcc acgtcgggcc cgccccagac
    16081 tcgctggcgg ccgcggcatc ggagccgact gtgcgatcgg tggtcgtcat cgacacgacc
    16141 ccggggccgc tgccgccgcc cgatctggtc gatgcggtgc tcgggttctc acgggaccat
    16201 tccgagctga ccgtcgtgat ctcggcggtg ggcagcgagc ggccggcccg gctcgtcgac
    16261 gacgcgaccg gggtcgcggt tcccctcttc accttccccg agcacgccgg caacgccctc
    16321 ggtcggctgg ccaccgcccg tgagtggcgg tcgaccgccc gggtctacgg cgacgagacc
    16381 ccgtcgggcc tcgacgtgga cgcgacacgg gcgctggtgg agcggtggcg ggccgacggg
    16441 ggaccggacc aggtgctcga gctcgacgcg tcgcgccagg agcagctgct ggcggcctgc
    16501 gggctgcggg tggcggatcg gagggaggtc cggaccgtcg agcaggccgt cgccgcggcg
    16561 gaggagatcg gctggcccgt ggcgctcaag gcccggcgac gcgaccgccg caagcgcacc
    16621 gcgctcagcg gcgtcgcgct cgacgtcgcc ggagtcgatg acctctccgc gacctgggcc
    16681 cgcatggagg aggcgctcgg gccggagggc atgcagccgg tggtggtcca gcggatggtg
    16741 gagcagggtc tcgacgtcgc ggtgcgcatc cgccggccgg atcggatcgg gaccgtcgag
    16801 gtgggcctgg gcggtccgat ggcggcgttc gatccgtggg agctgggtgt cctcccgctc
    16861 gccctgcccg acgcgtcgat gctggtggcg gcctcgtcgg tggcccgggc gttgaccgac
    16921 ccgctcaacc gggtgccggt cgtgtcgctc gtgcaccgct tggcggcgct ggtcgacgcg
    16981 gtcgaggaga tccacgaggt ccgcgccgat ccggtcatcg tggccggtcc cgtggcgtgg
    17041 atcaccgacg tgcggatcct gatcggctcc ccgcgcggcg agctaccggt ccgccacctg
    17101 ggttgaccgc tccggtcggg gttcacgggg gagccccagg acccgctcgc cgacgatgtt
    17161 gcgcaggatc gacgtggttc cgcccatgat cgtgtaggcg ggcgcgtagc agacgttgcg
    17221 ggcgacgcgg tcccacagga gggcatccgc gccggcgacc cgtgcgcaca ggtccgcgac
    17281 gcgctgctcg aactcggtgc agaacgtctt cgtcaccgcc gagaacccgg ggggcgcctg
    17341 gccgaggacc tcgcgcagga ccatgaggcg cccgatccgg tagccggact cgagcgcggc
    17401 gacctcctgg cgtacgagcg ggtcgtcgtg gtgcacggca ccgacgacat cgaggtacag
    17461 gcgccggttc gacaccagcc ggtcgatccc gccccgttcg tgctccatct ggcgcatgac
    17521 ctgcttgaag ctgccgttct cggcgccgac caggttctcg gtcggcacct cgacgccgtc
    17581 gaggtgcacc tcgcagaagt gggcgttgcc ggtggcgtcg acgatcgggc ggatgtcgat
    17641 ccccggggag tgcaggtcga cgacgagttc ggacagcccg gcgtgggcgg gggcgtcggg
    17701 gtcggtgcgg gcgatcaggt agcaccagtc ggcgtccgcg gcacccgagg tccagatctt
    17761 ctggccgttc accacccagc ggtcgccgcg gcgcacggcc ggggtccgca gcgatgccac
    17821 gtcggagccg gcgtcgggct cgctcatccc aatcgaccag gcggagcgcc cggcgatgat
    17881 ctcggggagc caccgggcgc gctgctcggg ggtgccgaac tgcagcaggg tcggtccgat
    17941 ctgacggtcg gcgaaccacg atgtcgccag cggtgcaccc tcggagatga gcgcctcgaa
    18001 caccgcgaag cgctccagcg cgtttcggcc gccgccgcct tcctcgaccg gccaggtcat
    18061 gcccagccag ccccgttcgg cgagctccag ggagaactcc cgggaggtgc cgatcagcca
    18121 ggagtcctcg cgcaccgctc gcgacgcggc cgcggcacgg ccgacccggt gggcttcggc
    18181 ggacagctcg gcgatggggt cgggccagcg gaagtccatg gcggggccgt cgagccggtt
    18241 cggtgcccca gcggacggtg gaaccggtcg ctcagcgacg ctcgatcggc gtgtagtccc
    18301 ggaccggatg gccgacgtag agctggcggg gccggtagat gcggctgtcc tggtcgagca
    18361 gctccaggta gtgcgacagc catccggcgg tgcgggggat ggcgaacagc acggtgaaca
    18421 tgtccatcgg gaagcccatc gcctggtaga tgagacccga gtagaagtcg acgttggggt
    18481 agaggcgacg gtcggtgaag tacgggtccg acagcgccac ctcttcgagc ttgagggcga
    18541 tgtcgagcaa ggggttcttg ccggtgacct cgaacacctc gtcagcggtc ttcttgatga
    18601 tccgggcccg ggggtcgtag ttcttgtaga cccggtggcc gaagccctgg agccggatgt
    18661 cgccggcctt gacccgctcg acgtaggcgg ggacgtgctc gatgtcgccg atctcggtga
    18721 gcatccggat gaccgcctcg ttggctccgc cgtggcgcgg accgtagagc gcggcggtgg
    18781 ccgcggcggt ggccacgtac gggtcggcgt gcgcgctgcc gacggtgcgc atggcggtgg
    18841 tggagcagtt ctgctcgtgg tcggcgtgga ggatgaacag cacgtcgagc gcccgggcca
    18901 ggaccgggtt ggcgtcgtag cggggctcgg cggtcttcca catcatcgac aggaagttgg
    18961 cggcgaagtc caggctgttg tccgggtaga cgaagggcat cccgacgctg tggcggaacg
    19021 ctcccgcggc gagggtcggc atcttggcga tcaggcggac gacctgcttc atcatgacgt
    19081 cgggcgtcag ctcgcgcgcc tctgggtaga acgtcgacag cgcggcgatg gtcgacacga
    19141 gcatgcccat cgggtgtgcg tcgtggtgga agccctccat gaagcgcttg cgcacgttct
    19201 cgtggatgaa cgtgtggtgg gtgatctcgt agcgccagtt ctcgaactgc tccggggtgg
    19261 gcagctcgcc gtggatcagc aggtaggcga cctcgaggta gctcgaggac tcggccagct
    19321 gctcgatcgg gtagccgcgg tagcgcagga agccggcctc gccgtcgagc tcggtgatcg
    19381 acgacgccgc accggaggtg gatccgaagc cggggtcgtg gaaccagatc cccggcagga
    19441 gcttggacca cgccttggcg tcgataccgc cgtcgaggat cggtacctcg agcgattccc
    19501 ccgtccggtt gtcggtgatg gtgatgctgt cggccacgtg cgtctcctgg gggaaggatc
    19561 gtccggtgcc atcctacggc gctgggagcc gtgtgctcgc cgtcactgcc tcgacgctgc
    19621 atcccggcca gctccgtcgc cgcccgggaa cagtgcggcg gcgttcaggt cgccggtcgc
    19681 agagtcgggt agacgccggg aaccgatgct gcgccctcgg cgatggttcg gcggatctgc
    19741 tcggcgacga cgcgctgggc ggcggtgggc agccccctgc gccgtcgggc cagtccgacg
    19801 ctgcggccgg ggacgccctc gaccgggatc cgccgccagg tcgcctcgac gttcgggggg
    19861 atggcggacg cggggacgac tccggccccg aagccggcga acaccagcga ggccagcagt
    19921 cgcatcccgt cgagctcggc cttggggcgc aggaccacac cctcggcggt ggccgcctgg
    19981 tcgagcacgt cgcggaacgc cgtgccccgg gcctcgagca gcagttcgtg gcgggagagc
    20041 tccgacaggg tgaccgagtc gtggtcggcc agaggatggc cgtcggggac gatcaggatg
    20101 cggtcctcgt cgaagagggg ctcgaccagc aggtcggtct cgtcgaccgg gaggttcacg
    20161 atggcgaggt caaccgctcc agacgccagg ttcggcacga gcgaggtcgt ggtggcatcg
    20221 agcacgatca cccggatctc ggggtactgg gcggtgacag aatcgaggag gggagcgacc
    20281 agccagcggg cggtggtgcc gatcgctccg agtcggaccg atccggacac gacgtcgcgg
    20341 agcgacgcga cgtccgcgtc gagggcttcg aactcgccct cgatgcgacg ggcccgctcg
    20401 aagaccacgc ggccctcgtc ggtgagctcg ttggtggagc ggtcgatgag cagcaccccg
    20461 agttgcttct cgagccgtgc cacgtgggcc gacacgttgg actggacggt gccgagcacc
    20521 cgagccccgg ccgagaacga ccgctgctcg gcgacggcga cgaatgctcg cagctgcttc
    20581 cgatccatcg ccagccacga taccaactat ctcccagata ttcttgaccg attgcttgct
    20641 cctggctatc gtggtgctca ccaagaacga ccgaggtcag aggacatccc cccccgtccc
    20701 actcggttca gcaccacggt ccatcccccc ctggaccgta ggggcactgc tgaaggactg
    20761 gctccccccg gccagtcctt cagccttttt ccaccgctcg acggggtgat ccgccatccc
    20821 acgacgccgg ccggaccgat aggttccaca tcagtgtcac tctggtcggt gtctgggagc
    20881 ttggctcgta ggttctctgc cgatc
//
LOCUS       DAOQOD010000040        20286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_911_length_20286_cov_13.123968, whole genome shotgun sequence.
ACCESSION   DAOQOD010000040 DAOQOD010000000
VERSION     DAOQOD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_20286_cov_13.123968"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLZ72_05935"
     CDS             complement(<1..1136)
                     /locus_tag="PLZ72_05935"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HNA35870.1"
                     /translation="MTDIDALIEAMTVEEKLAQLGCVWSTHLVDDHGFSPDAARRLLA
                     HGTGQITRIAASTGLRPQGVAVFANEIQRFLVEETRLGIPAVIHEESTAGFCARDAVQ
                     FPQAIGLGATWDRELVHEIADHIRTEMVAVGARQSLAPVLDVARDPRWGRVEETYGED
                     PVLAGELGTAYVIGLHGTMPESPADAAASVRARDGVIATAKHFLGYGLPEGGRNHGPV
                     QLGSRELREVFAEPFAAAIRDAGLSSVMNSYSSVDGLPCASSALILIELLRGELGFTG
                     TVVADYFSVDLLRTFHRVAPTKAVAAAKALLAGMDVELPALDCYAELPPLIEAGIVPI
                     GVVDAAVRRVLSQKEALGLFDDPFVDSDAAPAAFGTAGGVALARR"
     gene            1194..1556
                     /locus_tag="PLZ72_05940"
     CDS             1194..1556
                     /locus_tag="PLZ72_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNA35871.1"
                     /translation="MADVVIFHNPNCSTSKYAVGVASDLGVEVDERRYLLKAQRPTRD
                     EIVALLGQLEDPVTDLVRRDAQFAKLGLTDADVASADQVADVLAEHPQLLQRPVLVKD
                     GRAIIGRPKDRVEPFLRG"
     gene            complement(1602..2192)
                     /locus_tag="PLZ72_05945"
     CDS             complement(1602..2192)
                     /locus_tag="PLZ72_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35872.1"
                     /translation="MPTAHSVATRPNQRDHILDTALRLMSERGAKGMTMRQLASACDL
                     QVAAIYHYFESKDALLAAVVAERQYGSRMSDAFPIDPAADAADRLRSLFGHVWAGALE
                     EQSIWRLLISEGMHGEAAVLPVGQALLELLEPATRGWIERFVPEVDDPSTAAKLIVGQ
                     LLTGFVRHVFEPDRDPVAVGAECAEPVVVAVFGPGA"
     gene            2564..3673
                     /locus_tag="PLZ72_05950"
     CDS             2564..3673
                     /locus_tag="PLZ72_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35873.1"
                     /translation="MPGKRRNLVRGFGPICVALLAVVSACSGDERVVANADTALSVEP
                     LAQGFVAAAAPDSIVAAGGLHLDGSKATIGSSVWKWRTESATWSALPHLPDNLLAVGV
                     ASVGANTFVVARDCTSFPEATDLASDCHRTVVVRLVDGSWTVVEPPHDVVNPVSSIGL
                     VGQVGQRYLLLELSGSPTAFFAVFDVQTSKWSTVPRPELPAEQMMTGLCFSDGHILGY
                     ATGIVSSPEVTSDAVSLVALDEHLQWSKVLTPGKVKFPRNNSSSLCAADSLILQTTAD
                     EMTVLFVDSNDEPALRAIPPPAPIEALPGPGVPPLGTIDRQIVSVPRDGKEQLSSLGS
                     LSSGNPILGLSDTARAYVLYDVGSDKMPAVQLVGD"
     gene            3772..4512
                     /locus_tag="PLZ72_05955"
     CDS             3772..4512
                     /locus_tag="PLZ72_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35874.1"
                     /translation="MTFNIAGAAGAPYTTADHSNSITSGDPYLRQQHAMPIVYIAQAS
                     QPTGIAVQEDCDNTDPNITDAREYLMWELVSRGYQYFGVSTKTLTTRHCVQGTWIFAK
                     GTYAWADYLWLPGTTEGESRAAVCMRTVVFVQVANCSSHLTTAAGDQQQQSFSFWAFA
                     EQQVPYSFAVWSGGDFNTNNLNVLYPWSTNSSEGDTTLARNTFGSKKIDYSWGRLPIG
                     QAAGGSVVAGAPNYSDHNYVFAPFNWVY"
     gene            5185..5289
                     /locus_tag="PLZ72_05960"
                     /pseudo
     CDS             5185..5289
                     /locus_tag="PLZ72_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032728035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(5413..6768)
                     /locus_tag="PLZ72_05965"
     CDS             complement(5413..6768)
                     /locus_tag="PLZ72_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA35875.1"
                     /translation="MTILEDPSAASDRDFAQADFCDPHTFDDAWDLYAWLRQQPGLHH
                     DKKNDLFIAARHEDVFTVSRDSEMYCCRYGVRPAIAGDMSIITLDGEEHIRQRRLINQ
                     GFTPRRVREMLPHIRELANETIDAMLEKVTAEAAAAGADVGQARIDFVEDFAIHVPLI
                     IICELLGLDPDQRLSMYKWSDDMMAGDGHVDGDDPKLIAAAEAFGEYATMLIGLIAER
                     RENPTDDIISALTQAFDEGGLAREHEKAYQGVTKEELESREGFTGLNDDELLAFLTVL
                     LVAGNETTRNAISGGLMALSKFPEQKQLMIDNLWDDEFMDRAIDELIRYVTPVLGFIR
                     TVTADHDYQGTALAEGDRVLMLYAAANRDAAVFDRPDELDLTRESNPHLAFGIGQHFC
                     LGSNLAKAEVKLVFQELLSRIPDIEVPEGTVAGRGESSLVIALEDIPATFDASKCPVA
                     H"
     gene            6906..8162
                     /locus_tag="PLZ72_05970"
     CDS             6906..8162
                     /locus_tag="PLZ72_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA35876.1"
                     /translation="MTTTDPVAISDVDLLDLDAFVEGRHHAMFATLRQQDPVHFHAEP
                     DGPGFWAVTRHHDLQAVNRDTTTFSSQLGGTQRVDPDPEEFDTRGVLLLDSDPPQHTR
                     YRRLVNKGFTPRMIGLLEQHLRYRAELIVDSVIEQGSCEFVSDLAAELPLQAIAEIMG
                     VPQEDRRLLFDWSNAMIGSQDPEYQDPDNPDRPLQAAGEIFAYANDLASQRRDDPRDD
                     IVTTLINAEIEGEKLSELEFDMFMLLLTVAGNETTRNATSAGMHALLTHPDEMSRFLG
                     HLDDDAYRSVALDEVLRWATPVLHFRRTAAVDTEIRGREIAAGDKVLIWHVSANRDEE
                     VFDDPFRFDLERRPNEHVAFGGGGAHYCLGANLAKLELDIIFAEVLSRMPDLSAAGPA
                     EMLRSNFIGGVKHLPVSFTPGPRKHG"
     gene            complement(8198..9322)
                     /locus_tag="PLZ72_05975"
     CDS             complement(8198..9322)
                     /locus_tag="PLZ72_05975"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNA35877.1"
                     /translation="MRTSLCDQLDIEFPIFAFTHCRDVVAAVSNAGGLGVLGAVGFTP
                     EQLATELEWLDEHCHRPYGVDVVIPGKYEGMGELDPVKLEAELQTLVPEEHKDFARKL
                     LADHDVPELPADQIAPTLIGWTEATASPLIDEALRHDGVKLIANALGTPPPEVIERIH
                     GAGRLVAALCGAPAQAMRHKNAGVDIIIAQGTEGGGHTGDVASLVLWPQVIDAVAPTP
                     VLAAGGIGNGRQMAAALAMGAEGVWTGSLWLTVEEADIPPAQMQSYIDATSRDTVRSR
                     SWTGKPCRMLRNDWTDAWENPENPQPLGMPLQFMVTGEAVARGHAYPEQAKDVNFNPV
                     GQVVGQMNKVRRSRDVVLGMVEEYLEATGRLNELNERAGA"
     gene            complement(9428..10957)
                     /locus_tag="PLZ72_05980"
     CDS             complement(9428..10957)
                     /locus_tag="PLZ72_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNA35878.1"
                     /translation="MDPTNAPPAPTPEVPARHARSAPRARRALTALAAVVLALGAWGV
                     SVPTSSAVTGFTFAGSGWGHGVGMSQWGARGLAEQGSSAAQILGHYYSGTTVTTTSTA
                     TIRVLLATNSTLTLTPWGPTTFVAGAALGTTSAPVTVTSSGSTIQLSGGLNTSATGTV
                     SIALGGPPLEVSPPRVKYNRGFLQLHPTGSGTVQAVVTLPMQDYLYGLGEMPSSWPDA
                     ALQAQAIAGRTFAQKKVAEVAGAGTYDIVGGLPDQSYLGWDKEYGSLGQRWVAAVDAT
                     ASQVVTYGGGLIDAVYSASSGGHTENSETVWVAAVPYLRGVPDPADLTGGNPNAMWSR
                     TYNGAQLGAWFGVGEVTSIQVLGPVGVSGRLDKATIRLIGTAGSRDLIGSSFRSIVNT
                     NSPSLQLMSSRFTVSGATPGPSVPSNSPPIGIFTMAKAEGSTVVVGGAAGDPDGAPRL
                     RVVSTMGRETAVREVQAHPAGLWMAQWTGGKGTRTVCVWVLDNPTGASTALGCRDITV
                     K"
     gene            11054..>11262
                     /locus_tag="PLZ72_05985"
     CDS             11054..>11262
                     /locus_tag="PLZ72_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35879.1"
                     /translation="MTPIVGAVGVQSEVTPSGSAGVRVATANIRHGRPEGAAGPDPAR
                     MADTLGRIGADVLAVQEVDRRTRRAG"
     assembly_gap    11263..11362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11363..11724
                     /locus_tag="PLZ72_05990"
     CDS             <11363..11724
                     /locus_tag="PLZ72_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNA35880.1"
                     /translation="HLSTDPGVAVTQLRRALRSLDELAAGRPALLLGDLNLEARVVAP
                     VLVEAGFVAAPSGPTHPARRPTRRIDWVLVRRATVRSSEVLDVTCSDHRPLVATVERV
                     DGSGTVRTDVDADGRVP"
     gene            11771..12607
                     /locus_tag="PLZ72_05995"
     CDS             11771..12607
                     /locus_tag="PLZ72_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA35881.1"
                     /translation="MEREPDELAATPHQSDADRPPVGEPLVVSRHNPGARPAWTGSSD
                     VPAGAAEPAAAPVASMEGRVAVVTAATGALGVALAAEMVGRGARVLLVDDDLDGLLDV
                     VDSLAPARAVPVRCDLGSDADVAAACDFVTRSTAVDVVVHVGRHDGDDAEVRAVGRNA
                     LDDRYRADVRGPLGLIDGLSPVPGSAPTVVLVDPPRSTSDPWGPVATDLVAEHLPVRD
                     GVRVTRATCSEDLDPAEFAGAVIDLLGRDDVGVSRVELVGLPGPAPAVAPTPSAGTWL
                     DG"
     gene            12635..>13060
                     /locus_tag="PLZ72_06000"
     CDS             12635..>13060
                     /locus_tag="PLZ72_06000"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HNA35882.1"
                     /translation="MARTCMRPGCDRPAAARVAFDPVALQLWLDPLARRSAPVQELCE
                     FHVERLTVPRGWSATDRRPGVVADVVPGSPGLSVVDAAPSSTPVPEPEAESEPESEPD
                     APIDAGEPMAEPPVADLPVVSEPVVSEPVVSEPVVSEPVV"
     assembly_gap    13061..13160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13229..14860
                     /locus_tag="PLZ72_06005"
     CDS             13229..14860
                     /locus_tag="PLZ72_06005"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNA35883.1"
                     /translation="MVDFAFQEMFPQAKGDTPYRQLTSDFVEVDDYRGQRILRVDRQA
                     LTLLAAEALRDIAHLFRPGHLAQLAKILDDPEATENDRFVARTMLQNACVSAGMVLPS
                     CQDTGTAIVMGKKGQQVWTHSSDGAPGGDEEALARGVFRTYTESNLRYSQVVPITTYD
                     EVNTGTNLPAQIDLMAVDGDEYHFLVMAKGGGSANKTFLFQETKALLNPESLMSFVAE
                     KIRTLGTSACPPYHLAIVIGGTSAEMNLKTVKLASARYLDGLPTEGNGLGHAIRDPEL
                     EADIWRLAADTGIGAQFGGKYFVHDVRVIRLPRHGASCPVGIGVSCSADRQSVAKITE
                     AGVFVEQLETDPAQYLPEVTDDHLADEVVHVDLNRPMEQIRAELSRYPIRTRLSLSGP
                     MVVARDIAHAKLKERIERGEGLPQYVKDHPIYYAGPAKTPDGMASGSFGPTTAGRMDS
                     YVDLLMANGGSFVTLAKGNRSAQVRRACQTHGGFYLGSIGGPAAILAQDAIKHVEVLE
                     YPELGMEAIWRIEVEDFPAFIITDDKGNDFFADLL"
     gene            14960..15994
                     /locus_tag="PLZ72_06010"
     CDS             14960..15994
                     /locus_tag="PLZ72_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35884.1"
                     /translation="MRAIIVGVAAAAALLATSCQPPPGSSTPGAGTWAVNPSATDASI
                     AGDAVSTHLAYQPTVAAVGKLAVIFPGTSATTLAFSELTRALTGVGYHVIVLRYPNSI
                     GTTAACPDSNAATDPDCFRVFRSEVVFGAGVDDPDGQAYDHPTASVNQSNSVVNRLTK
                     LLDHLSAVAPAAGWGQFQVRSGATCTQVDPTYGGCAIDWSKVTVVGHSQGAGVGLYLA
                     KFFPLHRVVMLSGAFDAYDLGGGNYAVAPWITEGPLAVPAANIGSLIHTSDPNAARIR
                     AVAQLVGIPGPEADVSTGPSFGGSRQLVTGIPSACPIGDSAPSHNSTAVDLCVPDYAY
                     DAAWRYLATS"
     gene            16031..16699
                     /locus_tag="PLZ72_06015"
     CDS             16031..16699
                     /locus_tag="PLZ72_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNA35885.1"
                     /translation="MEITLFTNLLGAFGLGAASGLNPWIPLLGLGLAQRLGVVELSSS
                     FDWLGATPTLLVLVALLVLDLIGDKVAVVDSVLHAVGVVIAPASGAVVFAAQENLLSN
                     THPVLAGGVGLVLGGTVHAARSVLRPAVTATTGGVGNPVISAVEDVVSTVLTVLAVVV
                     PVLAFLTLVGLVAWAVVVLRRWRARRLRDRQARAGAARGPGGVTGASDGSPSTPAPGQ
                     PGLP"
     gene            16961..18712
                     /locus_tag="PLZ72_06020"
     CDS             16961..18712
                     /locus_tag="PLZ72_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA35886.1"
                     /translation="MADLIIRNGTVVDGTGAPATPGDVAVTDGIITEVGPDLAGRGAR
                     EIDADGRLVTPGFVDIHTHFDGQVTWDPIVAPSSLHGVTTIAMGNCGVGFAPAAPDRH
                     AWLISLLEGVEDIPGTALAEGLTWDWESFPEYLDSVAAKPHTVDIGAHVPHAALRTYV
                     MGERGAEHTEAPTEGEIARMAALVEEALAAGAMGFATSRTEVHRTSEGTNIGTLTAGD
                     AELLAIAEAMRRAGTGVTQLISDLYQTPDDEVAQRELDLLERFARTSGRPLSFTMQQA
                     YHSPERWRFQMDWVDRMVAAGLDVKAQVGVRPIGVLLGLQATANPFLLTPSYGEVAGL
                     ALPERVAALRDPERRRRILAEHAELVAGLEPGILRHIVGGVDLTFEMADPVDYELHTD
                     RSLGARARATGVDPTEMVYDLLLERDGEQLLYLPLFNFAHGDFGDLFEMITAPNVLFG
                     LSDAGAHCGAICDASMTTSSLVVWSRDRRDGEPVPLERIVHGHTQRNARHVGWLDRGV
                     VAPGYVADLNVIELESLACHPPTIVVDLPAGGRRLMQTAEGYRHTIKSGVVTFSDGEH
                     TGELPGELLRGTRPTPT"
     gene            complement(18819..20246)
                     /locus_tag="PLZ72_06025"
     CDS             complement(18819..20246)
                     /locus_tag="PLZ72_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA35887.1"
                     /translation="MADEFDAVVVGSGPNGLVAALELAQAGWRVLVLERSDTLGGGAR
                     TAELTLPGFRHDVCSAIHPLGLASPALRDLGLEQVGVEWVQPDAPLAHALRPGHSVLQ
                     ERSLAATAVGLGVDGRPWERLFGPSVGSGLDLVDSLLDPLDLPPRHPVQLARYGMSGL
                     WSVSALARRRFRGDDGPALLAGLAGHSMLSLDAPITTGFGMLLGQLAHVVGWPMARGG
                     SQAIADGLVALIRALGGTVLTGEDVRSLDDLPPARAVICDVTPRQLIDIAGDRLPSAY
                     RRRLERYRYGPGVFKVDWALDGPVPWIDARTARAGTVHLGGTLAEVRASEEDVVSGRV
                     PQVPFLLLAQQSNFDPDRAPAGKHTLWGYCHVPSGCDVDMTEAMEARIEQFAPGFRDL
                     VLARHTMSAPQFEAYNPNYVGGDINGGVADLRQFLFRPVPSLDPWSTPVEGLYLCSSS
                     TPPGGGVHGMCGRAAARSVLRRQPL"
BASE COUNT         2958 a         6965 c         7177 g         2986 t
ORIGIN      
        1 cgccgggcca gggccacacc gccggcggtc ccgaacgcgg ccggagcggc gtcggagtcc
       61 acgaacgggt cgtcgaagag gccgagcgcc tccttctggg acaggacccg gcgcaccgcg
      121 gcgtcgacga cgccgatcgg cacgatcccc gcctcgatca ggggtggcag ctcggcgtag
      181 cagtcgagcg cgggcagctc gacgtccatg cccgccagca gggccttggc tgccgcgacc
      241 gccttggtgg gggcgacgcg gtggaaggtt cgcagcaggt cgaccgagaa gtagtcggcc
      301 acgaccgtgc cggtgaagcc gagctcgccg cgcagcagct cgatcaggat gagcgccgac
      361 gacgcacacg gcagcccgtc gaccgacgag taggagttca tgaccgagct cagcccggcg
      421 tcgcggatgg ccgccgcgaa cggctcggcg aacacctcgc gcagctcccg ggatccgagc
      481 tgcaccgggc cgtggttgcg gccaccctcg ggcaggccgt agccgaggaa gtgcttggcg
      541 gtggcgatca ccccgtcgcg ggcccgcaca gaggctgccg cgtcggcggg actctccggc
      601 atggttccgt gcagcccgat cacgtaggcg gtgccgagct cgccggcgag caccggatcc
      661 tcgccgtagg tctcctcgac ccgcccccac cgggggtcgc gggcgacgtc gaggaccggc
      721 gccaggctct ggcgcgcgcc gaccgcgacc atctcggtgc ggatgtggtc ggcgatctcg
      781 tggaccagct cacggtccca ggtggcgccc agaccgatcg cctgggggaa ctgcaccgcg
      841 tcgcgggcgc agaacccggc ggtcgactcc tcgtggatca ccgcggggat gccgaggcga
      901 gtctcctcga ccaggaaccg ctggatctcg ttggcaaaca cggcgacgcc ctggggacgc
      961 aggccggtcg acgcggcgat ccgggtgatc tggccggtgc cgtgcgcgag cagccgtcgg
     1021 gccgcgtcgg gggagaagcc gtggtcgtcg accagatggg tcgaccagac gcagccgagc
     1081 tgggccagct tctcctcgac ggtcatggcc tcgatcaggg cgtcgatgtc ggtcatggcc
     1141 acgatcttgg caggccgttc cgacatctgc ggtcgcccgc taccgtgcgg cccatggccg
     1201 acgtcgtgat cttccacaac ccgaactgca gcacctccaa gtacgcggtc ggagtggcct
     1261 cggacctggg ggtggaggtg gacgaacgcc gctacctgct caaggctcag cgtcccaccc
     1321 gcgacgagat cgtcgcgctc ctcggccagc tcgaggatcc cgtcaccgac ctggtccgcc
     1381 gcgacgcgca gttcgccaag ctcgggctca ccgacgcgga cgtcgcgagc gccgaccagg
     1441 tggccgacgt gctcgccgag cacccgcagc tgctgcaacg tccggtgctg gtcaaggacg
     1501 gccgggcgat catcggtcga cccaaggacc gggtggagcc gttcctgcgg ggatgaccgc
     1561 gtagaacctg cgggcgtcga ccggggtcgg cgcccgctcg gtcaggctcc gggaccgaag
     1621 acggcgacga cgacgggctc cgcgcactcc gcgccgacgg cgaccgggtc gcggtcgggt
     1681 tcgaacacgt ggcggacgaa cccggtgagc agctgtccga cgatcagctt ggccgcggtg
     1741 gatggatcgt cgacctcggg gacgaaccgc tcgatccagc cgcgggtggc cggctcgagc
     1801 agctcgagca gcgcctggcc gaccggcagg acggcggcct cgccgtgcat gccctcgctg
     1861 atgagcagcc gccagatcga ctgctcctcg agcgcccccg cccacacgtg gccgaacagc
     1921 gaccggagcc ggtccgcggc gtcggcggcg gggtcgatcg ggaaggcgtc cgacatccgg
     1981 ctgccgtact ggcgctcggc gaccaccgcg gccagcaggg cgtccttgga ctcgaagtag
     2041 tggtagatcg ccgcgacctg caggtcacag gccgaggcga gctgccgcat ggtcatgccc
     2101 ttggcgccgc gctcggacat caaccgcagc gcggtgtcca ggatgtggtc gcgctggttg
     2161 ggtcgcgtcg cgacggagtg ggcggtcggc atggcctcag tgtgtcaggt gcggctcgcg
     2221 gcgggcgcga cccgacgggt gggtccgctc agcctcaatc caccgattcg gttcttgggg
     2281 tgacggtgac ggctgctggt ggttgccgtc tggtgatcgg gcggcgccgg tgtggttcgg
     2341 ggcgcgttga agctggcgtc gcggctcgtg ggttgtcggc gctcagggtg gtgtgggcgt
     2401 gcgccggccg ctgggcgggt tcagcctgac ggggcgggca acgcccggag ggcgttgagg
     2461 gtgtcggtga acggaaggca cggcgtcaat tgtcacacca gggcatctgt gcgaggctga
     2521 agctggcgag tcggccggag gcttcgagga agggcgcaat cgtatgcctg gaaagaggcg
     2581 gaacctcgtt cgcgggttcg ggcctatctg tgtggcactt ctcgctgtcg tctcggcgtg
     2641 ctcaggcgac gagagagttg ttgctaacgc tgacaccgca cttagcgtcg agcctctcgc
     2701 ccaaggattt gttgcggctg cggcgccaga ttcgatcgtc gccgccgggg ggctacacct
     2761 cgacggttca aaggcaacga ttggatcgtc tgtgtggaag tggagaacag aaagcgcgac
     2821 ctggtcggca ctcccgcacc tgcccgacaa cttgcttgct gtcggggtag cctcggtcgg
     2881 cgcaaacacg tttgtggtgg cgcgcgactg cacgtcgttc ccggaagcaa ccgacctcgc
     2941 cagcgattgt caccgcacag tcgttgtcag gttggtcgac ggctcgtgga ccgtcgtcga
     3001 acctccgcac gatgtcgtca accccgtgtc atcgatcgga ttggtcggcc aagtgggcca
     3061 gcgatacctg ctgctggagt tgtccggatc acctacggcg tttttcgccg tgtttgatgt
     3121 ccagacttcg aagtggagta cggttccaag acccgagctt ccggctgagc aaatgatgac
     3181 cggactgtgc ttctctgacg gacacatact cggatacgcg accggtatcg tctcctcccc
     3241 cgaggtgact tcggacgcgg tcagtttggt ggctctcgat gagcaccttc agtggtcgaa
     3301 ggtactcact ccagggaagg tgaagttccc acgaaacaac agctcgtcct tgtgtgcagc
     3361 tgattcgctt attcttcaaa ccacggcgga cgagatgacc gtgttgttcg tcgacagcaa
     3421 cgacgaaccg gcgctcagag cgattccacc tccggctccg atcgaagcgc ttccgggtcc
     3481 gggagttccc ccgcttggga ccatcgatcg gcaaatcgtc tcagttccgc gagatggcaa
     3541 ggagcaactg tcaagccttg ggtcgctctc ctcgggaaat ccgattcttg ggctgagcga
     3601 cacggcgagg gcctatgtct tgtacgacgt cgggagcgac aagatgcccg ctgttcaatt
     3661 ggttggcgac tgatgggacg tcgactgctc gcgaagacac tggtagtggg agcccttacc
     3721 ctcggagccg tcaatgtccc ggctggcgcg acggtcacac caacttcgta catgacgttc
     3781 aacatcgccg gcgctgcagg agctccgtac acaaccgcag atcattcgaa ctcgatcacg
     3841 agcggcgatc cctatctaag acagcaacac gcgatgccca tcgtgtacat agcccaggcg
     3901 tctcagccga cgggcatcgc tgttcaggaa gattgtgaca atacagatcc gaacatcacc
     3961 gatgcgcgcg aatacctcat gtgggaattg gtgtctcgag gataccagta cttcggtgtg
     4021 agtacgaaga cgttgacaac gaggcattgc gtccaaggca cgtggatatt cgccaaaggg
     4081 acgtacgcgt gggccgacta tctatggctt ccggggacaa cggagggtga atctcgagca
     4141 gccgtctgca tgcgtactgt cgtgttcgtc caagtggcca actgctcgag tcacctgaca
     4201 acggcggctg gggatcagca gcagcaatcc ttctcttttt gggcgtttgc cgagcagcaa
     4261 gtcccgtatt cattcgccgt gtggtcgggt ggcgacttta ataccaacaa cctcaacgtc
     4321 ctgtatccat ggtcaacgaa ctcctccgaa ggcgatacca cgctcgctcg aaataccttc
     4381 ggaagcaaga agatcgatta ttcgtggggc aggttgccga tcggccaggc tgcgggaggt
     4441 tccgtcgtcg ctggcgcccc gaactactcc gaccacaact atgtgttcgc cccgttcaac
     4501 tgggtgtact agtggtccgt cgctgaaata gcgagggcga gttcaagggg tgacggctac
     4561 accgtgaggt aggggttgag cctccaccgg tcagagcgtg atgcgggcga gactgcccgc
     4621 tgttcacaca caaccccgac cggtggagac gcccagcatg ccagcccaac cccctgtccg
     4681 cgcctgaacg cgaagagatc cgcgcaggga tcgaacgctg tgagaccgac tctgtgatcg
     4741 ccggccgtct cggccggcac cgcgccacga tcaacgccga gatcaaccgc aacggcggcc
     4801 gagcccgcta caccgcaacc gccgcgcaga cccgagccga ctcgtcgcgg gcccgcccga
     4861 aggccaccag gttcgacgct gatccggtac tcgccgcgca tgtgaccgaa cgcctcgagg
     4921 cgaaagacag cccgatgacg atctcgatcg agttggcccg cggaacccac ggcctggtcg
     4981 cctcgctcag ccacgagtgc atctaccagg ccatccacgc gcacggccga cgaggcctgc
     5041 gagccggact gcatgaagga cttcaccgca ggcgtcgatg ccgcaaacac cgggtgttcg
     5101 gcccgcccgc accgaagaag agccccctcg gcctgttcaa cccgatcagc ctccgaccag
     5161 gcgtcgcgtt gccgtggcga ggtcggccac ctcgaaggcg acctcatcat cggagccgcc
     5221 aacgcctccg cgttggtgac cttgttcgac cgggcaagcc gctacctctg gctcgcggac
     5281 ctgcccgacg gctaccacgc cgacggaact ctcgccgcgg tctgcgagat cctcgaccgc
     5341 agtcagaccg tcacagccgc cagtagaccc aggcacgtgg acgaccaccc cggcccgacg
     5401 gtggattgag gctcagtggg cgacggggca cttcgacgcg tcgaaggtcg ccgggatgtc
     5461 ctcgagcgcg atcaccagcg acgactcgcc gcggccggcg acggtgccct cggggacctc
     5521 gatgtcgggg atgcggctga gcagctcctg gaacacgagc ttgacctcgg ccttggccag
     5581 gttggagccg aggcagaagt gctggccgat cccgaaggcc aggtgcgggt tggactcgcg
     5641 ggtgaggtcg agctcgtcgg gccggtcgaa caccgccgcg tcgcggttgg cggccgcgta
     5701 cagcatcagg acccggtcgc cctccgcgag cgccgtgccc tggtagtcgt ggtcggcggt
     5761 gacggtccgg atgaacccga gcaccggggt gacgtagcgg atgagctcgt cgatcgcccg
     5821 gtccatgaac tcgtcgtccc acaggttgtc gatcatgagc tgcttctgct cggggaactt
     5881 cgacaacgcc atcaggccgc cggagatggc gttgcgggtg gtctcgttgc cggcgaccag
     5941 caggacggtg aggaaggcga gcagctcgtc gtcgttgagg ccggtgaagc cctcacggga
     6001 ctcgagctct tccttggtga cgccctggta ggccttctcg tgctcgcggg ccaggccgcc
     6061 ctcgtcgaag gcctgggtga gtgccgagat gatgtcgtcg gtcgggttct cgcgccgctc
     6121 ggcgatcaga ccgatcagca tcgtggcgta ctcgccgaac gcctcggccg ccgcgatcag
     6181 cttggggtcg tcgccgtcga cgtggccgtc gccggccatc atgtcgtcgg accacttgta
     6241 catcgacaga cgctggtcgg ggtcgagtcc gagcagctcg cagatgatga tcagcgggac
     6301 gtggatggcg aagtcctcga cgaagtcgat ccgggcctgg ccgacgtccg caccggccgc
     6361 ggccgcctcg gcggtgacct tctccagcat ggcgtcgatc gtctcgttgg ccagctcccg
     6421 gatgtggggg agcatctcgc gcacgcggcg gggggtgaag ccctggttga tcaggcggcg
     6481 ctggcggatg tgctcctcgc cgtcgagggt gatgatcgac atgtcgcccg cgatcgcggg
     6541 gcggaccccg tagcggcagc agtacatctc ggagtcgcgg ctgacggtga acacgtcctc
     6601 gtggcgcgcc gcgatgaaga ggtcgttctt cttgtcgtgg tgcagtcccg gctgctgacg
     6661 cagccatgcg tacaggtccc acgcgtcgtc gaaggtgtgg ggatcgcaga agtcggcctg
     6721 cgcgaagtcg cggtccgacg cggcggaggg gtcctcgagg atcgtcatga gaagaccgta
     6781 caagtgaccg aacgctcgtt caagggccaa tcgaacgatc gttcagagcc cggggtcggt
     6841 acgatcgtgg cgcgcccggt cgggaccacc ggtcccgcac gcggggcgcg ccgacagggg
     6901 ggcacatgac cacgaccgat cccgtcgcga tcagcgacgt cgacctgctc gacctcgacg
     6961 cgttcgtcga gggtcgtcac cacgccatgt tcgccacgct gcgccaacag gacccggtcc
     7021 acttccacgc cgagccggac ggcccggggt tctgggcggt caccaggcac cacgacctgc
     7081 aggcggtcaa ccgcgacacc accaccttct cgagccagct cgggggcacc cagcgcgtcg
     7141 acccggaccc cgaggagttc gacacccgcg gcgtgctgct gctcgactcc gacccgcccc
     7201 agcacacccg ctaccggcga ctggtcaaca agggcttcac cccccgcatg atcggcctgc
     7261 tcgaacagca cctgcgctac cgcgccgagc tgatcgtcga ctcggtcatc gaacagggca
     7321 gctgcgagtt cgtctccgac ctggccgccg agctcccgct gcaggccatc gccgagatca
     7381 tgggggtccc ccaggaggac cgccgcctgc tcttcgactg gtccaacgcc atgatcggct
     7441 cccaggaccc cgagtaccag gacccggaca acccggaccg cccgctgcag gccgcgggcg
     7501 agatcttcgc ctacgccaac gacctggcct cccagcggcg cgacgacccc cgcgacgaca
     7561 tcgtcaccac cctcatcaac gccgagatcg agggggagaa gctcagcgag ctcgagttcg
     7621 acatgttcat gctcctgctg accgtcgccg gcaacgagac gacccgcaac gccacctcgg
     7681 ccgggatgca cgcgctgctg acccaccccg acgagatgtc ccgcttcctc gggcacctcg
     7741 acgacgacgc gtaccggtcg gtcgcgctcg acgaggtgct gcggtgggcc acaccggtgc
     7801 tgcacttccg acgcaccgca gcggtcgaca ccgagatccg cggtcgggag atcgcggcgg
     7861 gcgacaaggt gctcatctgg cacgtgtcgg ccaaccgcga cgaagaggtg ttcgacgacc
     7921 cgttccgctt cgacctggaa cgccggccca acgagcacgt cgccttcggt ggcggcggag
     7981 cgcactactg cctgggggcg aacctggcca agctggagct cgacatcatc ttcgccgagg
     8041 tgctcagccg catgccggac ctgtccgcgg cgggaccggc cgagatgctg cggtcgaact
     8101 tcatcggcgg ggtcaagcac ctgccggtca gcttcacccc gggtccacgc aagcacggct
     8161 gaccggtcgg gtcagcggcc ggaccgctcg ccgagggtca ggccccggcg cgctcgttga
     8221 gctcgttgag ccggccggtg gcctcgaggt actcctcgac catgccgagc acgacgtcgc
     8281 gggagcgccg gaccttgttc atctggccga cgacctggcc gaccgggttg aagttcacgt
     8341 ccttggcctg ctcggggtag gcgtggccgc gggcgaccgc ctcgccggtg accatgaact
     8401 gcaacggcat gccgagcggc tgggggttct cggggttctc ccaggcgtcg gtccagtcgt
     8461 tgcgcagcat ccgacacggc ttgccggtcc acgaacggga gcgcaccgtg tcgcggctgg
     8521 tggcgtcgat gtaggactgc atctgcgccg gcgggatgtc ggcctcttcg acggtcagcc
     8581 acagcgagcc cgtccacacg ccctcggcgc ccatcgccaa cgccgcggcc atctgacggc
     8641 cgttgccgat gccgccggcg gcgagcaccg gggtcggggc gacggcgtcg atcacctggg
     8701 gccacagcac cagcgaggcg acgtcgccgg tgtggccgcc gccctcggtg ccctgggcga
     8761 tgatgatgtc gaccccggcg ttcttgtggc gcatcgcctg ggccggggcg ccgcacagcg
     8821 cggcgaccag ccgaccggcg ccgtggatgc gctcgatcac ctcgggcggc ggcgtgccca
     8881 gagcgttggc gatcagcttc acgccgtcgt ggcgcagcgc ctcgtcgatc agcggcgacg
     8941 cggtggcctc ggtccagccg atcagggtcg gggcgatctg atcggcgggc agctcgggaa
     9001 cgtcgtggtc ggccaggagc ttgcgggcga agtccttgtg ctcctcgggt acgagggtct
     9061 gcagctcggc ctcgagcttg accgggtcga gctcgcccat cccctcgtac ttcccgggga
     9121 tgacgacgtc gacgccgtag gggcggtggc agtgctcgtc gagccactcg agctcggtgg
     9181 ccagctgctc gggggtgaac ccgaccgctc ccaggacgcc gaggccgccg gcgttggaca
     9241 ccgcggcgac gacgtcgcgg cagtgggtga aggcgaagat cgggaactcg atgtcgagct
     9301 ggtcgcagag gctggtgcgc atggcggcac ggtagcggag aactggaacg tgtttcagtt
     9361 ctgctccgtc gagccgccgt cggccggacc taccggagca tgcgtcggga ccggccgacc
     9421 ggggttgcta cttgacggtg atgtcacggc acccgagcgc ggtggacgcc ccggtcgggt
     9481 tgtcgagcac ccacacgcac acggtccggg tccccttgcc gccggtccac tgggccatcc
     9541 acaacccggc gggatgcgcc tgcacctcgc gcaccgccgt ctcgcggccc atcgtcgaca
     9601 cgacccgcag acgcggggct ccgtcggggt cccccgcggc tccgccgacc acgaccgtcg
     9661 acccctcggc cttggccatc gtgaagatgc cgatcggcgg gctgttcgac gggaccgacg
     9721 gtcccggggt cgcgccgctg accgtgaacc tgctcgacat gagctggagc gacggcgagt
     9781 tcgtgttgac gatcgatcgg aacgacgacc cgatcaggtc gcggctcccc gcggttccga
     9841 tcagtcggat cgtcgccttg tcgagacgac ccgacacccc gaccggcccg agcacctgga
     9901 tcgacgtgac ctcgcccacc ccgaaccagg cgccgagctg ggcaccgttg taggtccgcg
     9961 accacatggc gttggggttc ccgccggtca ggtcggccgg atcgggcaca ccgcgcaggt
    10021 aggggaccgc ggcgacccac accgtctcgc tgttctcggt gtggccaccc gaggacgccg
    10081 agtacaccgc gtcgatcaac ccgcccccgt aggtcacgac ctgcgacgcg gtggcgtcga
    10141 ccgcggcgac ccagcgctgg ccgagcgatc cgtattcctt gtcccacccg aggtacgact
    10201 ggtcgggcag gccgccgacg atgtcgtagg tgccggcgcc cgccacctcg gcgactttct
    10261 tctgggcgaa ggtccgcccc gcgatcgcct gggcctgcag cgcggcgtcg ggccacgacg
    10321 acggcatctc gcccagcccg tagaggtagt cctgcatcgg cagggtgacg acggcctgga
    10381 cggtcccgga cccggtgggg tgcagctgaa ggaacccccg gttgtacttg accctcggcg
    10441 gcgacacctc gagcggcggt ccgccgagcg cgatcgacac cgtgcccgtc gccgacgtgt
    10501 tcagaccgcc ggagagctgg atggtcgaac cgctggacgt gaccgtcacc ggagccgacg
    10561 tcgtgcccag cgccgcgccg gcgacgaagg tcgtggggcc ccacggcgtg agggtcagcg
    10621 tcgagttggt ggcgagcagg acccggatgg tggccgtcga cgtcgtggtc acggtggtgc
    10681 ccgagtagta gtggcccagg atctgcgcgg ccgacgagcc ctgctcggcc aggccgcggg
    10741 caccccactg gctcatgccg acgccgtggc cccagccgga gccggcgaag gtgaacccgg
    10801 tgaccgccga gctggtcggg acgctcaccc cccaggctcc gagggcgagc accacggcgg
    10861 ccagggcggt cagggcccgg cgggccctcg gtgcggatcg ggcgtggcgt gccggaacct
    10921 ccggggtcgg agcggggggc gcgttcgtcg gatccatcgg gagcgatgcc acggtgactg
    10981 cctcggctcg actgtcctcc gacttgagga tcgggtcggg gtcaggggtc tcacgctgtg
    11041 cgacccttgt ggggtgaccc cgatcgtagg agccgtcgga gtgcagtcgg aggtcacccc
    11101 ttcggggagt gccggcgtgc gggtggccac cgcgaacatc cgccacggac gtcccgaggg
    11161 cgcggcgggc ccggatcccg ctcgcatggc cgacaccctc gggcggatcg gggcggacgt
    11221 gctcgccgtc caggaggtcg accggcgcac gcgacgggcc ggnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nncccacctg tccaccgatc cgggggtcgc ggtcacccag
    11401 ctccggcggg cgctccggtc gctcgacgag ctggcggcgg gtcgaccggc gctgctgctc
    11461 ggcgacctga acctggaggc ccgggtggtc gccccggtgc tggtcgaggc cgggttcgtc
    11521 gcggcgccgt cgggccccac ccatccggcg cggcgaccga cccgccggat cgactgggtg
    11581 ctggtgcggc gggcgaccgt gcgctcctcg gaggtgctcg acgtgacctg cagcgatcac
    11641 cgtccgctgg tcgcgacggt cgagcgcgtg gacggctccg ggacggtccg aaccgacgtg
    11701 gacgccgacg gtcgggttcc ctagcctcgc cgcagcgatc gagaggcgga gcgggcgggc
    11761 gatgcaccgg atggaacggg aacccgacga gttggcggcc acgccgcacc agagcgacgc
    11821 cgaccggccg ccggtcggcg aaccgctcgt cgtctcccgg cacaacccgg gggcccgccc
    11881 ggcgtggacc ggatcctctg acgtcccggc cggcgcggcg gagcccgcgg cagcgccggt
    11941 cgcctccatg gagggacggg tggccgtggt gaccgcggcc accggcgcgc tcggtgtcgc
    12001 tctcgccgcc gagatggtcg gccggggggc acgggtcctg ctcgtggacg acgatctcga
    12061 cggactgctc gacgtggtgg actcgctggc tcccgcgcgg gccgtaccgg ttcggtgcga
    12121 cctcggttcg gacgcggacg tggccgcggc ctgcgacttc gtcacgcgga gcacggccgt
    12181 cgacgtcgtg gtgcacgtcg gtcgacacga cggcgacgac gccgaggtcc gagcggtcgg
    12241 tcggaacgct ctcgacgacc gctaccgcgc cgacgtgcgt ggcccgctgg gcctgatcga
    12301 cggcctgtcc ccggtgcccg gttccgctcc gacggtggtg ctggtcgatc cgccgcgctc
    12361 gaccagcgac ccgtgggggc cggtggcgac cgatctggtg gccgagcacc tgccggtgcg
    12421 cgatggggtg cgggtcaccc gggcgacctg cagcgaggat ctcgaccccg ccgagttcgc
    12481 gggagcggtc atcgacctgc tcgggcgcga cgacgtcggg gtgtcacggg tcgagctggt
    12541 gggtctgcca ggaccggccc cggcggtggc cccgactccg tcggcaggta cctggctcga
    12601 cggttgagcg ggcgccgggc gatagcctgc gggagtggcg aggacgtgca tgcgaccggg
    12661 gtgcgaccgc ccggccgcgg cgcgggtcgc gttcgacccg gtcgccctgc agctgtggct
    12721 cgatccgctg gcccggcgat cggcgccggt gcaggagctg tgcgagttcc acgtcgagcg
    12781 gttgaccgtg ccccggggct ggtcggcgac ggaccgtcgg cccggagtcg tggccgacgt
    12841 ggttccgggc tctccgggcc tgtcggtcgt ggatgctgcg ccgtcgtcca cgccggttcc
    12901 cgagcccgag gcggagagcg agcccgagag cgagcccgac gctccgatcg acgccggcga
    12961 accgatggcc gaacctccgg tcgcggatct gccggtggtg tcggagccgg tggtgtcgga
    13021 gccggtggtg tcggagccgg tggtgtcgga gccggtggtg nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn ccgaccggag gtcgccggtc ggatcccggg ttgggtgcgg
    13201 caggaccggt ggtgcctact gtggcgccat ggtcgacttc gccttccagg agatgttccc
    13261 ccaggccaag ggggacacgc cgtaccgcca gctgacctcc gacttcgtcg aggtcgacga
    13321 ctaccggggt cagcggatcc tgcgggtcga tcgacaggcg ctgaccctgc tggccgccga
    13381 ggcgctgcgc gacatcgcgc acctgttccg ccccggtcac ctggcccagc tcgccaagat
    13441 cctcgacgac cccgaggcga ccgagaacga ccggttcgtc gcccgcacga tgctgcagaa
    13501 cgcgtgcgtg tcggccggga tggtgctccc gtcgtgccag gacaccggca ccgccatcgt
    13561 catgggcaag aagggccaac aggtctggac ccactcctcc gacggcgccc ccggcggcga
    13621 cgaggaggcc ctggcccgcg gcgtgttccg cacctacacc gagagcaacc tccgctactc
    13681 gcaggtcgtg ccgatcacca cctacgacga ggtcaacacc ggcaccaacc tcccggccca
    13741 gatcgacctg atggccgtcg acggcgacga gtaccacttc ctggtcatgg ccaagggcgg
    13801 gggcagcgcc aacaagacgt tcctgttcca ggagaccaag gcgctgctca accccgagtc
    13861 gctgatgagc ttcgtggccg agaagatccg caccctcggg acctcggcgt gcccgccgta
    13921 ccacctggcg atcgtcatcg gcggcacgtc ggccgagatg aacctgaaga ccgtcaagct
    13981 ggccagcgcc cgctacctcg acggcctccc gaccgagggc aacgggctcg gccacgcgat
    14041 ccgggacccc gagctcgagg ccgacatctg gcgtctggcc gcggacaccg gcatcggcgc
    14101 ccagttcggc ggcaagtact tcgtccacga cgtgcgggtg atccgcctgc cccgccacgg
    14161 ggcgtcgtgt ccggtcggca tcggtgtcag ctgctcggcg gaccgccaga gcgtcgccaa
    14221 gatcaccgag gcgggcgtgt tcgtcgagca gctcgagacc gacccggcgc agtacctgcc
    14281 cgaggtcacc gacgaccacc tggccgacga ggtcgtgcac gtcgacctca accgtccgat
    14341 ggaacagatc cgcgccgagc tcagccgcta cccgatccgg acgcggctgt cgctgagcgg
    14401 tccgatggtg gtggcccgtg acatcgccca cgccaagctc aaggagcgca tcgagcgggg
    14461 tgagggcctg ccccagtacg tcaaggacca cccgatctac tacgcggggc cggccaagac
    14521 gcccgacggg atggcgtcgg gttcgttcgg gccgaccacc gcgggtcgga tggacagcta
    14581 cgtcgacctg ctcatggcca acggcggcag cttcgtgacc ctggccaagg gcaaccggtc
    14641 cgcccaggtg cgccgggcgt gtcagaccca cggcgggttc tacctgggct cgatcggcgg
    14701 accggcggcc atcctggcgc aggacgccat caagcacgtc gaggtgctcg agtaccccga
    14761 gctgggcatg gaggcgatct ggcggatcga ggtcgaggac ttccccgcct tcatcatcac
    14821 cgacgacaag ggcaacgact tcttcgccga cctgctctga cccatgccac tgcggccggc
    14881 cgccaggacg gattgcgttg tgcgcctgcg ccgtttgtgg cagattttcc gccgccggag
    14941 ggagcgtgtc gtgggtcaca tgagagcgat catcgtgggt gttgcggccg cggccgccct
    15001 gctggcgacg tcgtgccagc ccccgcccgg gagctcgacg ccgggagccg ggacctgggc
    15061 ggtcaacccc agcgccaccg acgcgtcgat cgccggcgac gccgtctcga cgcacctggc
    15121 gtaccagccg accgtcgcgg cggtcggcaa gctggcggtg atcttcccgg gcacgtcggc
    15181 caccacgctc gcgttctcgg agctcacccg ggcgctcacc ggagtgggct accacgtgat
    15241 cgtgctgcgg tacccgaact cgatcgggac caccgccgcg tgccccgatt cgaacgcggc
    15301 caccgacccg gactgcttcc gggtgttccg ctccgaggtc gtcttcgggg ccggggtcga
    15361 cgaccccgac ggtcaggcct acgaccatcc gaccgcgtcg gtgaaccagt cgaactcggt
    15421 ggtcaaccgc ctcaccaagc tgctcgacca cctgagcgcc gtcgcacccg ccgccggctg
    15481 gggtcagttc caggttcgct ccggcgccac ctgcacccag gtcgacccga cctatggcgg
    15541 ctgcgccatc gactggtcca aggtgaccgt cgtcgggcac tcgcagggcg cgggcgtcgg
    15601 cctgtacctg gccaagttct tcccgctgca ccgggtggtc atgctctcgg gggcgttcga
    15661 cgcctacgac ctcggtggcg gcaactacgc ggtcgcgccg tggatcaccg aggggccgct
    15721 cgccgttccg gcggcgaaca tcgggagcct gatccacacc tccgacccga acgcggcgcg
    15781 catccgggcc gtggcgcagc tggtcggcat cccgggaccc gaggccgacg tgtcgaccgg
    15841 cccgtccttc ggcggcagcc gtcagctggt gaccggcatc ccgtccgcgt gtccgatcgg
    15901 tgactcggcc cccagccaca actcgaccgc ggtggacctg tgcgtccccg actacgcgta
    15961 cgacgccgcc tggcggtacc tggccacgtc ctgacggcgc ggcggctccg cgcccgggca
    16021 gactcccgcg gtggagatca cgctgttcac caacctgctc ggggccttcg ggctcggagc
    16081 ggcgtcgggt ctgaacccgt ggatcccgct gctcggcctc ggcctggccc agcgactggg
    16141 cgtggtcgag ctgtcgtcga gcttcgactg gttgggagcc acgccgaccc tgctggtcct
    16201 cgtcgccctg ttggtcctcg acctgatcgg cgacaaggtc gcggtcgtcg actcggtgct
    16261 gcacgccgtc ggtgtggtga tcgccccggc gtcgggagcg gtggtgttcg ccgcacagga
    16321 aaacctgctg agcaacacgc atccggtcct ggccggaggc gtcggcctgg tgctcggcgg
    16381 cacggtgcac gcggcccgct cggtgctgcg acccgcggtc accgccacca ccggtggggt
    16441 cggcaacccg gtgatcagcg cggtcgagga cgtggtgtcg accgtgctca ccgtgctcgc
    16501 ggtcgtggtg ccggtgctgg cgttcctgac cctggtcggt ctggtggcgt gggcggtggt
    16561 ggtgctccgt cggtggcggg cccgacggtt gcgggaccgt caggcgaggg ccggtgcagc
    16621 ccgtggtccc ggcggggtca caggagcgtc ggacggttcg ccgagcacgc ccgcgccagg
    16681 gcagcctggg ttgccctgaa gtcgcgcacc tgacgctcgg cggcggtggt gctcgggcgg
    16741 agcatccagg cgatgcggtg acggagtcgg ctggccatgc ctctccatcg gcgcatgcgg
    16801 ggttggatca agcggaaccc gggatcgggt tggtcctcgg gcctccgcgt ggcacgaccc
    16861 gctccggcgt ggcacggtgg cgggcgcgcc ccgacgtcgg ggagcagagg tggctcgggc
    16921 gcaccgcccc ggcgaccgtg gccgcaccag ggggaacgac atggccgatc tgatcatccg
    16981 aaacgggacc gtcgtcgacg gcaccggagc gccggcgacc cccggcgacg tggccgtgac
    17041 cgacgggatc atcaccgagg tgggcccgga tctcgccggt cggggcgccc gcgagatcga
    17101 cgccgacggc cggctggtca cacccggctt cgtcgacatc cacacccact tcgacggcca
    17161 ggtcacgtgg gatccgatcg tggcgccgtc gtcgctgcac ggggtgacca cgatcgccat
    17221 gggcaactgc ggggtcggtt tcgccccggc ggcgccggac cggcacgcct ggctgatcag
    17281 cctgctcgag ggggtcgagg acatccccgg caccgcgctg gccgaggggc tcacctggga
    17341 ctgggagtcg ttccccgagt acctcgactc ggtggcggcg aagccgcaca ccgtcgacat
    17401 cggcgcgcac gtgccgcacg ccgcgctgcg cacctacgtg atgggcgagc gcggcgccga
    17461 gcacaccgag gccccgaccg agggtgagat cgcccggatg gccgcgctgg tcgaggaggc
    17521 gctggcggcc ggagccatgg gattcgccac gtcgcgcacc gaggtgcacc gcaccagcga
    17581 gggcaccaac atcggcaccc tgaccgcggg ggacgccgag ctgctcgcca tcgccgaggc
    17641 catgcgtcgg gccggcaccg gcgtgacgca gctgatcagc gacctctacc agactcccga
    17701 cgacgaggtg gcccagcgcg agctcgacct gctggagcgg ttcgctcgga cgagcggtcg
    17761 accgctgagc ttcacgatgc agcaggcgta ccactcgccg gagcggtggc ggttccagat
    17821 ggactgggtc gaccggatgg tcgccgccgg gctcgacgtc aaggcgcagg tcggggtccg
    17881 gccgatcggc gtgctgctcg gtctgcaggc gaccgccaac ccgttcctgc tgaccccgtc
    17941 ctacggcgag gtggcggggc tcgccctgcc cgagcgggtg gcggcgttgc gcgacccgga
    18001 gcgccgccgt cggatcctgg ccgagcacgc cgagctggtg gccggtctcg agccggggat
    18061 cctgcgacac atcgtcggcg gcgtcgacct caccttcgag atggccgacc cggtcgacta
    18121 cgagctgcac accgaccggt ccctcggcgc ccgggcccgg gccacgggcg tggacccgac
    18181 ggagatggtc tacgacctgc tgctcgagcg cgacggcgag cagctgctgt acctgccgtt
    18241 gttcaacttc gcccacggcg acttcggtga cctgttcgag atgatcaccg cccccaacgt
    18301 gctgttcggg ttgtccgacg ccggcgcgca ctgcggggcg atctgcgacg cgtcgatgac
    18361 gacgtcgtcg ctggtggtgt ggagtcggga ccgtcgtgac ggcgagccgg tcccgctcga
    18421 gcggatcgtg cacggtcaca cgcagcgcaa cgcccgccac gtcggctggt tggatcgggg
    18481 ggtggtcgct ccggggtacg tggccgacct caacgtcatc gagctggagt cgttggcgtg
    18541 ccatccgccg acgatcgtcg tcgacctgcc cgccggcggt cggcggctga tgcagaccgc
    18601 cgaggggtac cggcacacga tcaagtccgg ggtggtcacc ttcagcgacg gggagcacac
    18661 cggcgagctg cccggagagc tgctgcgcgg cacccgcccc acccccacct gagcgcccgc
    18721 tcttcccgtt cttcccgttc tgtagtgcga gatccagggc gaacgcctgg atctcgcact
    18781 acagaacggg ttgtgggggg tgtcgcggga gtcgggggtc agaggggttg gcggcgcagc
    18841 accgatcggg cggctgcccg cccgcacatg ccgtgcaccc cgcccccggg cggtgtcgac
    18901 gacgagcaca ggtacaggcc ctccaccggg gtcgaccacg gatcgagcga cgggaccggc
    18961 cggaacagga actggcgcag gtcggccacg ccgccgttga tgtcgccgcc gacgtagttg
    19021 gggttgtagg cctcgaactg tggggcgctc atcgtgtggc gggccagcac caggtcccgg
    19081 aagccggggg cgaactgctc gatgcgcgcc tccatcgcct cggtcatgtc gacgtcgcag
    19141 cccgacggga cgtggcagta gccccagagg gtgtgcttgc cggccggggc cctgtcgggg
    19201 tcgaagttcg actgctgggc cagcagcagg aacgggacct gcggaacccg ccccgacacg
    19261 acgtcctcct cggaggctcg cacctcggcc agcgtgccgc ccaggtgcac cgtcccggcc
    19321 cgggcggtcc gcgcgtcgat ccacggcacc ggtccgtcga gggcccagtc gaccttgaac
    19381 acgcccggtc cgtagcggta ccgctcgagg cggcgacggt aggccgacgg cagccggtcg
    19441 ccggcgatgt cgatgagctg gcggggggtg acgtcgcaga tgaccgcccg tgcaggaggg
    19501 aggtcgtcga ggctccggac gtcctcgccg gtgagcacgg ttccacccag cgcccggatc
    19561 agcgccacga gcccgtcggc gatcgcctgc gatccgcccc gcgccatcgg ccagccgacc
    19621 acgtgggcga gctggccgag cagcatgccg aacccggtgg tgatcggggc gtcgagcgac
    19681 agcatcgagt gtccggccag ccccgcgagc agggcggggc cgtcgtcgcc gcggaaccgc
    19741 cgccgggcca gagccgacac cgaccacagc cccgacatgc cgtagcgggc cagctgcacc
    19801 gggtgccgcg gcggcaggtc cagggggtcg agcagcgagt cgaccaggtc caggcccgag
    19861 cccacgctcg gtccgaacag ccgctcccac ggtcggccgt cgacgccgag gccgaccgcg
    19921 gtcgcggcca gcgaccgctc ctgcagcacc gagtggcccg gacgcagcgc atgggcgagc
    19981 ggggcgtcgg gctgcaccca ctcgacgccg acctgctcga ggccgaggtc gcgcagggcc
    20041 ggggaggcca acccgagtgg gtggatggcc gagcagacgt cgtggcggaa gccgggcagg
    20101 gtcagctcgg cggttcgggc gccgccgccg agggtgtcgg agcgttccag gacgaggacc
    20161 cgccagccgg cctgggcgag ctcgagcgcg gcgacgagcc cgttgggacc gctgcccacg
    20221 accaccgcgt cgaactcgtc ggccaccgcc ccaccctacg cggccacccc gcaccccccg
    20281 cccacc
//
LOCUS       DAOQOD010000041        20240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_916_length_20240_cov_11.822690, whole genome shotgun sequence.
ACCESSION   DAOQOD010000041 DAOQOD010000000
VERSION     DAOQOD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_20240_cov_11.822690"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLZ72_06030"
     CDS             complement(<1..607)
                     /locus_tag="PLZ72_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNA35888.1"
                     /translation="MNPEMMEALDALAESRGISKDALFAALAEALEAAYKKQNDDYEF
                     VWVNIDTETGEMRVFAQELDEDNLPTGPEMDVTPDDFGRIAAQTVRQVMSQRIREVER
                     ELKYEEYAGREGDIVTGIIQQSDARYTLLDLGRVEALLPQSEQVNYERPEPGERVKAY
                     IVEVRKTVKGPQIVVSRTHPGLIRRLFELEVPEIADGVVEIK"
     gene            complement(604..1182)
                     /gene="rimP"
                     /locus_tag="PLZ72_06035"
     CDS             complement(604..1182)
                     /gene="rimP"
                     /locus_tag="PLZ72_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNA35889.1"
                     /translation="MSTTADRLARLVTPVADDLSVTVYDIEDSSGTLRVLLDRPDGID
                     VDTLADASRRISRAMDEDGSFASAAVLEVSSPGLERKLRTAAHFLGAVGEQVKIKLAP
                     GVADRRIDGVVAAADDRTVTVATPDGGPDRTLLLADITSAHTVFVWETTPKPGTKRTV
                     PASNHSGDARPRSDRDPQPAEPADPAETEATA"
     gene            complement(1438..2871)
                     /gene="proS"
                     /locus_tag="PLZ72_06040"
     CDS             complement(1438..2871)
                     /gene="proS"
                     /locus_tag="PLZ72_06040"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNA35890.1"
                     /translation="MAASTSGRKAPVLTPQSEDFPRWYQDVLNKAELADNGPVRGTMV
                     IRPYAYALWERMVDEVDRRIKAAGAENAYFPLFIPESYLQREAEHVEGFSPELAVVTV
                     GGGRELEEPVVVRPTSETVIGEFMAKWVQSYRDLPLLLNQWANVVRWELRPRTFLRTS
                     EFLWQEGHTAHATEGDARAYARRILAEVYRDFMVEVLAIPVITGVKIPQERFAGATNT
                     LTCEAMMRDGKALQMGTSHELGQNFARAFQIDYLDETGTQQLCWTTSWGVSTRMMGGL
                     IMAHGDDAGLRVPPRLAATQAVVLVVRDEDGAVTAAARGLVDRLVAAGVRARLDDRIS
                     TPFGRRATDWELKGVPVRIEVGPRDLAEGQVVVVRRDRPGKEPIPVERLAQEVPVLLS
                     SIQQSLHDEALARRDDRTVDATTVEEAASAAADGFVRIPWAAVGDAGVERLAADAVTV
                     RCLITADGDVPESVEDPDLVAVVARSY"
     gene            2918..3919
                     /locus_tag="PLZ72_06045"
     CDS             2918..3919
                     /locus_tag="PLZ72_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA35891.1"
                     /translation="MARSRLGTVGWMAVGAGAATAAAWAAAEVSARRIRSRTDPANDA
                     LRPPPSDVVHRSIDTADGASLHVVERGQGRPLLLMHGVTLQWWVWGAQFNTLADHYRV
                     LAWDMRGHGRSTVGSEGITLEAIADDIAAVLEDLDLRDAILVGHSMGGMALGRFCARH
                     EDLMIERCGGLMFLDTSAAPLALPGRLDGNVRAEELVRRAAYAGTSRPRVRYGWPDST
                     PSVLMARSAFGRRPSATAVLDVREMLSVMDQDNLVAAGRAIVDHDVRSDLAHVKVPAM
                     VVVGSADRLTPVAQARVLADSIPGSVLRVLPGIGHQTMQEDPVRFAELVDELSTLSP"
     gene            complement(3944..4882)
                     /locus_tag="PLZ72_06050"
     CDS             complement(3944..4882)
                     /locus_tag="PLZ72_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNA35892.1"
                     /translation="MDPLLELAEQQHGLITLGQARSLGIGQQTVHRWASGPSWERLSD
                     EVLRRRGSAPNRAQHVLAAVLDAGPGAFLSFASGAAWWRVPGPPLDPIGIVRVSRTSR
                     PSGTGAEIHTVRSLPERWTTTLDAVPIVRPELLALHLFATEPYARAERWVERLWAMRL
                     LSGPSLGAMLCDFGARGRNGIASVRRYLDDRGPGYVPAATGIESRTMQILRQVGIVVE
                     RQVDLGGEESWTGRVDFLVVGMPIIIEVQSELHHAALVDRVADDARRARLEADGFIVV
                     ELWDTEVWSHPSVAVARVAAAVATHRSVRSITTSEL"
     gene            complement(5002..6231)
                     /gene="ispG"
                     /locus_tag="PLZ72_06055"
                     /gene_synonym="gcpE"
     CDS             complement(5002..6231)
                     /gene="ispG"
                     /locus_tag="PLZ72_06055"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNA35893.1"
                     /translation="MDAGAVVFPRRPTRQIQVGSVLVGGGAPITVQSMTTTRTSDVDG
                     TLQQIYALAANGADIVRCTCNEMEAAEGLARMVPRSPLPIVADIHHQYRMALAALEAG
                     VQGLRLNPGNIRNPAHIKAVAVEARDRGVPIRIGVNGGSLDPALYERHGGRVTPEAMV
                     ESALQEIAYFDEVGFDLIKISVKASNVPLMIEAYRQLAEATDHPLHLGVTEAGPLPDG
                     LIKATAGIATLLAEGIGDTIRFSLTADPVEEAKAGRTLLESFGLRERKNVDLIACPSC
                     GRAEVDVIQIAEAARDAFAEKQLPLQIAVMGCVVNGPGEARDADLGIAAGRHRGHLFV
                     KGTNVAVVPEDEMVATLVEWAEFIAEHGTDAALARVDVEKARREAEKDRSAMFADKGA
                     DVNASEQRIEQIHRRLD"
     assembly_gap    6235..6334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6338..7984)
                     /locus_tag="PLZ72_06060"
     CDS             complement(6338..7984)
                     /locus_tag="PLZ72_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA35894.1"
                     /translation="MTIHDPGSGQDGGPAESSLARTPSTQPGHDPAALAEERNEMAGV
                     LGTPLTRALRLGVLLALLVGLGVWKGLPIVIVILAIVLMIFLHELGHYLAARWSGMKV
                     TEFFIGFGPRIWSFRRGETEYGIKAIPAGAYVKIPGMTSLEDVDPADEARSYRQAPFH
                     NRLAVAVAGSTMHFLVAFLLLIVQFAFIGHPDGDRWQIGNISPGSAAQAAGLHEGDRL
                     VRFDGRTVEDYDSFRSLIGGHDPGTVDVVIDRDGQEMTVPVTLSQRARVIGTVGEDLD
                     LVDNGSELVVGSMRVDGQVARSGLRPGERVVAVAGRPVDSADGVVDAIGSGGSAVDGG
                     TFTVTTETADGVRSDHQVDLGSAIDTTAPTAFLGIGSEPIIVTDGLPTAVASSVTEFG
                     RYVGATVAGTGRVLWPPNIVGFLADTATGSEPRDTVSTPTPAAQSQSPAEERPVSIVG
                     IVLFGSEMTAESWSNLIGLLIGLNIMLGVLNLIPLLPFDGGHVAIAVYEKAQEMRRRQ
                     TRRYLADVSHLLPVAYGVVMVLGLLFVAAMYLDVTQGVSL"
     gene            complement(7981..9150)
                     /gene="dxr"
                     /locus_tag="PLZ72_06065"
     CDS             complement(7981..9150)
                     /gene="dxr"
                     /locus_tag="PLZ72_06065"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNA35895.1"
                     /translation="MTGSLTTVSLLGSTGSIGTQTIDVVAAEPDRFRISVLGAGSDVV
                     TLAEQARSVRPDVVVVADRSRAAELRSLVASDIEVDAGPDAFAAAAGSADVVVNAVVG
                     FAGLPVTMAALSSGRRLALANKESLIAAGPVVRTVRDTPGAEIVPVDSEHCAVHQCLR
                     SGDGTRRLRRIVLTASGGPFRGRTAADLADVTREDALAHPTWSMGPKITIDSSTLMNK
                     GLEVIEAHELFDVDYDDIDVVVHPQSIVHSMAEFTDGATIAQLSMPDMRLPIGYALAY
                     PDRIGTPFGSMDLTRSFELTFEPPDRATFRCLDLAERAGRAGATAPAWMSAANEVAVD
                     AFLAGRIAWSGIAELVERALDRWDGTPADSLEAVLAADAAARAVTTELLDREVAA"
     gene            complement(9306..10985)
                     /locus_tag="PLZ72_06070"
     CDS             complement(9306..10985)
                     /locus_tag="PLZ72_06070"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNA35896.1"
                     /translation="MTDHDDPGRIEEPRSEGVRIIGAQEAAEAAGRSDVARRRRTGEK
                     RYGDRPDSAAPGEGLPTVRISTSDSGDDAFDSGEVVHPPSQEPRWADDEGRGFGHARA
                     IPADEPDPYAPVDDVPDWDRSPAPADDPFAGSDDSFVLPHWTEPPTGQVPKVVTGDDF
                     VEDEPTGSFGATPRWRDEGERHVETDFDDLLDDAPRLGALGGSDPRSGYDDDDDDYFF
                     DSESDRDPIEAFAPDDDVSALSEPRAPRRTRSRPRMTAVDGGSGDGEEHPPDSGGVGG
                     DRNLVTAVGVGVALVAVGLLCFKLGGLATTALACVVVGVAAYEYFGTLEGRSFQPAGL
                     LGMVATVGLMIATYTSGLNAYPIVLPLTVALGLLWYLFVQPGEGSVRNLGVTLLGVMW
                     IGFLGSFATLLLGLGKVGEDAGASSNIGIGVLIAAVVVAVSHDVGAYFIGRYFGHTPL
                     SAASPNKTLEGTAGGVGVAIVVTVVVVGIFGIAPIGGDFFRVVVFALLCALVAPIGDL
                     CESFVKRDLGIKDFGTILPGHGGVLDRFDALLFVLPTAYFVTILFGTWTGG"
     gene            complement(11017..11586)
                     /gene="frr"
                     /locus_tag="PLZ72_06075"
     CDS             complement(11017..11586)
                     /gene="frr"
                     /locus_tag="PLZ72_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNA35897.1"
                     /translation="MSDELIEAVLEDAGDHMDKAVAHARHEFAGVRTGRANPGLVEKL
                     PVDYYGASTPLQQLASFSVPDARMLVITPFDKGAMGGIERAIQDSGLGLNPSNDGVAI
                     RLAFPPLTEERRKEYVRLVKSKAEDGRTAVRGARRDARKELDSLEKDGDISSDDLHRA
                     EGELDKLTKKHEAAIDEALEAKTVELLEG"
     gene            complement(11675..12391)
                     /gene="pyrH"
                     /locus_tag="PLZ72_06080"
     CDS             complement(11675..12391)
                     /gene="pyrH"
                     /locus_tag="PLZ72_06080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNA35898.1"
                     /translation="MATPHWGRVLIKLSGEAFAGEAGFGIDGEVVSAIASEIVAVKSD
                     FDVDVAVVVGGGNIWRGMTGEGAGMDRAQADYMGMLATIINGLALQETLERLGQPTRV
                     QTAIQMAQVAEPYIRRRAIRHLEKGRVVIFVGGTGNPFMTTDTTAALRAVEIDADAIL
                     MGKNGTDGVYTADPKLDPDAEFLAEVSYIDVLNRGLKVMDSTAISLCMDNDLPICVFD
                     LMGSNLHAILAGERVGTLVR"
     gene            complement(12394..13185)
                     /gene="tsf"
                     /locus_tag="PLZ72_06085"
     CDS             complement(12394..13185)
                     /gene="tsf"
                     /locus_tag="PLZ72_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNA35899.1"
                     /translation="MADITAADVKSLRDATGAGMMDAKKALVEADGDRQKAAQILRQK
                     GLSKVATLEDRENNQGAVAIATQGNAASLVELKSETDFSAKAADFVELTQRLADAVLA
                     DGEGAVASLSDDLDSLKIAKRENIEIGKVARFEAADGNVLDAYVHIQDGRGTNAVLIE
                     IEGTDAETAHEVALHIAFAKPSALSRDEVDPALAETARSGFEELTLKEGKPEQAVPKI
                     VEGRMSSWYAERVLPEQGMFGDKETVQQRLGGARIVRFAQAVIGD"
     gene            complement(13185..14171)
                     /gene="rpsB"
                     /locus_tag="PLZ72_06090"
     CDS             complement(13185..14171)
                     /gene="rpsB"
                     /locus_tag="PLZ72_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNA35900.1"
                     /translation="MSAVVSMKQLLEAGVHFGHQTRRWNPKMKRFIHGERGGIYLIDL
                     HQTLTGIEKSYVFVRDLVANGGTVLFIGTKKQAQDSIQGYAEKCGMPYINQRWLGGML
                     TNFETISKRVSKMKEYQRMRDSGEFEAMPKKEALLLTRELDKLERNLNGIRDLERLPD
                     AVFILDTKKEDIAVTEANKLGIPVVAVVDTNCDPDIIQYVIPGNDDAIRSGSLLTRVI
                     ADAVLEGRLMRSKTHPESAPRERSAEEQAEKAAEQARAQAEARQAAAEREARVLAARE
                     APADESSVLAEGAAAEDITSEAPTANQVAADELALDGEPAAASPVTPKTEEN"
     gene            complement(14396..14920)
                     /locus_tag="PLZ72_06095"
     CDS             complement(14396..14920)
                     /locus_tag="PLZ72_06095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA35901.1"
                     /translation="MRTALVAIVSLLLSGPSVAAPVPAHGPAVPVVGTASMEPTPGPG
                     GYRPPVDGDVVDPFRAPINPYGPGNRGLEYATDEGAPARSIGDGVVVFAGPVARRGVV
                     TVEHPDGLRSSLTGLATIGVAPGERVAAGDVLGTTGLRLHLGVRRGDEYIDPASLFGA
                     EIPPLHAVLVPLPP"
     gene            complement(15055..15861)
                     /gene="whiG"
                     /locus_tag="PLZ72_06100"
     CDS             complement(15055..15861)
                     /gene="whiG"
                     /locus_tag="PLZ72_06100"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HNA35902.1"
                     /translation="MTTRTRTEDTTTDEELARLWSSYKDKGTTRAREALIVHYSPLVK
                     YVASRLAVGLPQNVEQSDLVSYGMFGLIDAIDKFEPERGYKFETYAISRIKGSILDEL
                     RSIDWVPRSVRAKVRQIERAYAKLEAKHHRAPSDEELASELRWTDDQLQTALNQIANV
                     GLAALDEILTGSGERGESITLGDTIADRSSHGPMGAFEVAETRQLLAQAVNGLPEREK
                     IVLTLYYYESLTLQEIGRVLGVTESRVCQIHTKAVLHLRSRFNSMDRIPG"
     gene            complement(15861..16742)
                     /locus_tag="PLZ72_06105"
     CDS             complement(15861..16742)
                     /locus_tag="PLZ72_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA35903.1"
                     /translation="MAAVALAPSTLAVYARDVSSFARWAEADPQEPVRRPDLVDRDRI
                     RRYLASLSASGRAPRTMARTMASLRRYFTWCQRRGLVSVDPTVGVSAPSGAARLPRVL
                     RADELRRLLDESPDTEDPVVTARDTAVLELLYGSGLRVSELCGLDVDQVDLARSVITV
                     WGKGSKQRIVPLSGPARDALVDWLAPTGGRVSFLTDVAGSVPLDTDGAAVFHNRRGRR
                     LGPRDVRRLIDRRSPVPTHPHALRHTFATHLLDGGADLRVVQELLGHADLATTQVYTH
                     VSKDRLSRVFRESHPRA"
     gene            complement(16909..18162)
                     /locus_tag="PLZ72_06110"
     CDS             complement(16909..18162)
                     /locus_tag="PLZ72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNA35904.1"
                     /translation="MTPPRRPSGTPDGPDADLDDRSVPLEAYLGALATLDGMGSASLR
                     TLLALGSPSEVWERICRGAVDARLRNALAPVARDASGSWAAQARSVDPVALWTRWRSR
                     GIGAVSIGSPSYPACLLDDLDPPVLLFHRGDLDALAHPRVAVIGTRRATGYGRRVAHE
                     LGLDLASAGVCVVSGLALGIDAAAHNGAVAASDRALGSTAETHVDPSVSGRTQAGPVA
                     VVGGGVDAPGPSTNAALARRVVDVGAVLSEAPPGTRPAPWRFPVRNRILAALAPVLVV
                     VESAERGGSMLTVEQAQLRDRTVLAVPGPVDSPASAGTNALISEGALVCRGADDVVSA
                     LGLSLEGVANSPVENRPEPDGDAASVLAVLGFTPVSIESLSRDLDLDLGRLSVALGRL
                     EALGWAERRGPFVERVARSDGGPRR"
     gene            complement(18159..19670)
                     /locus_tag="PLZ72_06115"
     CDS             complement(18159..19670)
                     /locus_tag="PLZ72_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNA35905.1"
                     /translation="MLAIAHSATLIGARGYPVRVEAHVSDGLPSFAIVGLPDASCRES
                     RDRVRAALLSSKFVVKQARTTVNLAPTHLRKAGAGLDVAIALALMVATGQLDAELLDG
                     HAFIGELGLDGSMRPVIGTLPLCEAAGDLRPVVAHADAAEAALVRPDARCARTLSELV
                     GALRGDLPWPDGEPRRPHGSGPPDEPDLRDVQGQTVARRALEVAAAGGHHLLMVGPPG
                     GGKTMLAERLPGLLPDLTDQAALDVSRVHSAAGALEDTSRLLRRPPMRAPHHTASMVA
                     LVGGGSTVLRPGEISLASGGVLFLDELGEFPAAHLDALRQPLESGVVRVARAHVGASL
                     PARFLLVAATNPCPCGVGPFGPCRCSAAQIQRYQRRLSGPLLDRFDLGLWVDPPPASE
                     VLGQRSAGESTAVVRDRVARARERARERGISANRQLRGEALERAAPLSAEALAVLRDH
                     LAAGRLTMRGAQRIRAVALTLMDLDGLDGPLDAERVQVALSMRTHAQALGVAA"
     gene            20015..>20240
                     /locus_tag="PLZ72_06120"
     CDS             20015..>20240
                     /locus_tag="PLZ72_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35906.1"
                     /translation="MSGSPRRGLDHTATAECEVRRVQPYEATKLYLCPGCARDIPPGT
                     GHVVAVPLDAPDLRRHWHRGCWDKRLTRAPR"
BASE COUNT         2875 a         7031 c         7299 g         2935 t
ORIGIN      
        1 ccttgatctc gacgacgccg tcggcgatct cgggcacctc gagctcgaac aggcggcgga
       61 tcagtccggg atgggtgcgc gacacgacga tctgcgggcc cttgacggtc ttgcggacct
      121 cgacgatgta ggccttgacc cgctcccccg gctcggggcg ctcgtagttg acctgctcgg
      181 actgcggcag cagggcttcg acccgaccga ggtcgagcag cgtgtagcgg gcgtcggact
      241 gctggatgat cccggtgacg atgtcgccct cgcggccggc gtactcctcg tacttgagct
      301 cgcgctcgac ctctcggatc cgctgggaca tgacctggcg gaccgtctgg gccgcgatgc
      361 gaccgaagtc gtcgggggtg acgtccatct cgggaccggt cggcaggttg tcctcgtcga
      421 gctcctgggc gaagacgcgc atctcacctg tctcggtgtc gatgttgacc cagacgaact
      481 cgtagtcgtc gttctgcttc ttgtaggccg cctcgagcgc ctcggcgagg gcggcgaaga
      541 gggcgtcctt ggagatccca cggctctcgg cgagggcgtc gagcgcctcc atcatctctg
      601 ggttcatgct gtggcctcgg tttcggctgg gtcggcgggt tcggctggtt gcgggtcgcg
      661 gtcggatcgc gggcgggcgt cgcccgagtg gttcgacgcg gggacggtgc gcttcgtccc
      721 gggcttgggg gtggtctccc acacgaacac ggtgtgggcg gaggtgatgt cggcgaggag
      781 gagcgtccgg tccggtccgc cgtcgggggt cgcaacggtc accgtgcggt cgtccgccgc
      841 ggccacgacg ccgtcgatgc ggcggtcggc cacgccgggg gcgagcttga tcttgacctg
      901 ctcccccacc gctccgagga agtgcgccgc ggtgcggagc ttgcgctcca gacccgggct
      961 cgacacctcg agcacagctg ccgaggcgaa ggatccgtcc tcgtccatgg cccggctgat
     1021 gcgccgggag gcgtcggcga gggtgtccac atcgatgccg tcggggcggt cgagcagtac
     1081 ccgcaaggtg ccgctcgagt cctcgatgtc atagacggtc acgctgaggt cgtcggcgac
     1141 gggcgtgacg aggcgcgcga gtcggtcagc cgtggtggac accgtgctcc tcctcgttcg
     1201 tcgtctgccg ctggcgcgga tgctcctttc cccgcggacg cggggtcgga tcacgcaaaa
     1261 ggcgtgggcg acgcccacgc cttccacgga tccggacggg tcgagtatag gcggaccgac
     1321 gactccgccg cggatcggcc gggatggcgc gtcacgggtg cgcaccgtgg ccgggagtcc
     1381 ggtcgcgccg cggtcggcga tgggttccct ggcacctcgg gcacaccggc tccggtctca
     1441 gtaggagcga gccaccaccg cgaccagatc cggatcctcg accgactccg ggacgtcgcc
     1501 gtccgcggtg atgaggcaac ggaccgtgac cgcatcggcg gccagccgct cgaccccggc
     1561 gtcgccgacc gccgcccacg ggatgcggac gaagccgtcg gcggcagcgc tcgccgcctc
     1621 ctcgacggtc gtcgcgtcga cggtccggtc gtcgcgacgg gcgagcgcct cgtcgtgcag
     1681 agactgctgg atcgagctca ggagcaccgg gacctcctgg gccaacctct cgacggggat
     1741 cggctccttg ccgggtcggt cacgtcggac gaccacaacc tggccctcgg cgaggtcgcg
     1801 cggcccgacc tcgatgcgga ccggaacccc cttgagctcc cagtccgtgg cccgtcggcc
     1861 gaacggcgtg ctgatccggt cgtccaggcg ggcgcggacg ccggctgcga cgagccggtc
     1921 gaccagccca cgggcggccg cggtgacggc tccgtcctcg tcgcggacga cgaggacgac
     1981 ggcctgggtg gccgccagcc ggggcgggac ccgcaggccg gcgtcgtcgc cgtgggccat
     2041 gatcagcccg cccatcatgc gggtggacac gccccaggac gtcgtccagc acagctgctg
     2101 ggtcccggtc tcgtcgaggt agtcgatctg gaacgcccgg gcgaagttct gaccgagctc
     2161 gtggctggtg cccatctgca gtgccttgcc gtcgcgcatc atcgcctcgc aggtgagcgt
     2221 gttcgtcgct ccggcgaagc gctcctgggg gatcttcacc ccggtgatca ccgggatggc
     2281 gagcacctcg accatgaagt cgcggtacac ctcggccagg atccgtcggg cgtaggcgcg
     2341 ggcgtcaccc tcggtggcgt gggcggtgtg gccctcctgc cagaggaact cgctggtgcg
     2401 caggaacgtg cggggccgca gttcccagcg cacgacgttg gcccactggt tcagcagcag
     2461 cggcaggtca cggtaggact gcacccactt ggccatgaac tcgccgatga cggtctcgga
     2521 ggtcggccgc accacgacgg gctcctcgag ctcgcgaccg ccgccgacgg tgacgaccgc
     2581 cagctcgggg ctgaagccct cgacgtgctc ggcctcacgt tgcaggtacg actcggggat
     2641 gaacagcggg aagtaggcgt tctcggcccc ggcggccttg atccggcggt cgacctcgtc
     2701 gaccatccgc tcccacaggg cgtacgcgta cgggcggatc accatcgtgc cgcggaccgg
     2761 gccgttgtcg gcgagctccg ccttgttgag gacgtcctgg taccagcggg ggaagtcctc
     2821 ggactggggc gtgagcaccg gcgccttgcg gccggatgtg gaggctgcca tggcggcgga
     2881 gcctaccggc gaccgcgacc gggcctacgc tgcgtccatg gctcgatctc ggctcggcac
     2941 ggtgggatgg atggcggtcg gggccggtgc ggccaccgcg gcggcgtggg ccgcggccga
     3001 ggtgtcggcc cggaggatcc gctcgaggac cgatcccgcc aacgacgctc ttcgtccccc
     3061 gccgtccgat gtcgtgcacc gctcgatcga caccgcggac ggcgcctcgc ttcacgtcgt
     3121 cgagcgcggc cagggccgac cgctgctgct gatgcacggc gtgacgttgc agtggtgggt
     3181 gtggggggcg cagttcaaca ccctggcgga ccactaccgg gtactggcgt gggacatgcg
     3241 cggccacggt cgctcgacgg tgggttccga gggcatcacg ctcgaggcga tcgcggacga
     3301 catcgccgcg gtgctcgagg atctggacct gcgcgacgcc atcctggttg gccactcgat
     3361 gggtgggatg gcactgggac ggttctgcgc ccgccacgag gacctcatga tcgagcgctg
     3421 cggcggtttg atgttcctcg acacgagcgc cgctcccctg gccctgcccg gtcgcctcga
     3481 cgggaacgtc cgggcagagg agctggttcg tcgggcggcg tacgccggaa ccagtcggcc
     3541 gcgggtccgc tacggctggc ccgacagcac cccgtcggtg ctgatggcgc gctcggcgtt
     3601 cggccggcga cccagcgcca ccgcggtgct cgacgtccgg gagatgctgt cggtcatgga
     3661 ccaggacaac ctggtggcgg ccggtcgggc gatcgtggac cacgacgtcc gttccgatct
     3721 ggcgcacgtg aaggttccgg ccatggtcgt ggtcggctcg gcggaccgac tcaccccggt
     3781 ggcccaggcc cgggtgctgg ccgactcgat ccccggttcg gtgctgcgtg tgctgccggg
     3841 gatcgggcac cagaccatgc aggaggaccc cgtccgcttc gccgagctcg tcgacgagct
     3901 cagcaccctc tccccctgac cgcgacccgt tctgttcgtt cgatcacaac tccgacgttg
     3961 tgatcgaacg aacagaacgg tgggtggcga cggctgcggc gactcgtgcc accgcgacgg
     4021 aggggtgcga ccacacctcg gtgtcccaca gctcgacgac gatgaagccg tcggcctcga
     4081 gccgggcccg ccgggcgtcg tcggccaccc ggtcgaccag cgccgcgtgg tggagctcgc
     4141 tctggacctc gatgatgatc ggcatgccga cgaccaggaa atcgacccgc cccgtccagg
     4201 actcctcacc gccgaggtcg acctgtcgtt ccacgacgat cccgacctgc cgcaggatct
     4261 gcatggtgcg cgactcgatc ccggtggcgg ccgggacgta gcccgggccg cggtcatcga
     4321 ggtagcgacg aaccgaggcg atcccgttgc gcccgcgggc gccgaagtcg cacagcatgg
     4381 caccgagcga cgggcccgac aggagccgca tcgcccagag gcgctcgacc caccgttcgg
     4441 ctcgagcgta cggctcggtc gcgaacaggt gcagcgccag cagctcgggg cgcacgatcg
     4501 gtacggcgtc gagcgtggtc gtccatcgct ccggcagcga tcggacggtg tggatctccg
     4561 ctccggtgcc cgacggtcgg ctggtccggg agacccggac gatcccgatc ggatccaggg
     4621 gcggacccgg gacccgccac cacgccgcac cgctggcgaa cgacaggaac gctcccggac
     4681 cggcgtcgag caccgcggcg aggacgtgct gggcccggtt cggcgcggat ccacgacggc
     4741 gcaggacctc gtcggagagc cgttcccacg acggaccgga tgcccagcga tggacggtct
     4801 gctggccgat cccgagcgaa cgggcctggc cgagggtgat cagcccgtgc tgctgctcgg
     4861 cgagctcgag gagtggatcc atgtccacga cgatggcgaa cgacccgccc cgcgtgaaga
     4921 ggggaccgct ccccggtcgc cccaccccgt tctgttcgtt cgttcacaac ggtggtggtg
     4981 tgatcgaacg aacagaacgg gtcagtcgag gcggcggtgg atctgctcga tgcgctgctc
     5041 ggacgcgttc acatcggcgc ccttgtccgc gaacatggcg gagcggtcct tctcggcctc
     5101 gcggcgggcc ttctcgacgt cgacgcgggc cagggccgca tcggtcccgt gctctgcgat
     5161 gaactcggcc cactcgacca acgtcgccac catctcgtcc tcggggacga ccgccacgtt
     5221 ggtgcccttg acgaacaggt gaccgcggtg gcggcccgcc gcgatgccca gatcggcgtc
     5281 gcgggcctct ccggggccgt tgaccacgca gcccatcacc gcgatctgaa gaggcagctg
     5341 cttctcggcg aaggcgtcgc gggccgcctc ggcgatctgg atgacgtcga cctcggcccg
     5401 accacagctc ggacatgcga tcagatcgac gttcttgcgc tccctcagac cgaaggactc
     5461 gagcagggtg cgcccggcct tggcctcctc gaccggatcc gcggtgagcg agaagcggat
     5521 cgtgtcgccg atgccctcgg cgaggagcgt ggcgatcccg gcggtggcct tgatcagccc
     5581 gtcgggaagc gggccggcct cggtcacgcc gaggtgcaac gggtggtcgg tggcctcggc
     5641 gagctgacgg taggcctcga tcatgagcgg cacgttcgat gccttgaccg agatcttgat
     5701 caggtcgaaa ccgacctcgt cgaagtaggc gatctcctgg agggcggact cgaccatggc
     5761 ctcgggggtg acccgtccgc cgtgtcgttc gtagagcgca gggtcgagcg atccaccgtt
     5821 gaccccgatg cggatgggga cgccccggtc cctggcctcg accgcgaccg ccttgatgtg
     5881 ggccgggttg cggatgttgc cggggttgag ccgcagcccc tgcaccccgg cctcgagggc
     5941 ggccagcgcc atgcggtact ggtggtggat gtcggcgacg atcggcagcg ggctgcgcgg
     6001 caccatgcgg gccaggccct cggcggcctc catctcgttg caggtgcagc gcacgatgtc
     6061 ggccccgttg gcggccaggg cgtagatctg ctgcaacgtg ccgtcgacat cggaggtgcg
     6121 cgtcgtggtc atcgactgca ccgtgatcgg ggcgccccct cccacgagca ccgagcccac
     6181 ctggatctgt cgggtggggc gccggggaaa gacaaccgct cctgcgtcca tgcgnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgcta caggctgacg ccctgggtga
     6361 cgtcgaggta catggccgcg acgaacagca gaccgagcac catgaccacg ccgtaggcca
     6421 ccggcagcag gtgcgagacg tcggcgaggt agcgacgggt ctgacgacgg cgcatctcct
     6481 gggccttctc gtagaccgcg atggccacgt gaccgccgtc gaagggcagc agcgggatca
     6541 ggttgagcac gcccagcatg atgttgaggc cgatgagcag tccgatcagg ttggaccagc
     6601 tctcggcggt catctccgaa ccgaacagga cgatgccgac gatggacacc ggccgctcct
     6661 cggccggcga ctgcgactgg gcggccgggg tcggcgtcga caccgtgtcg cggggttctg
     6721 acccggtggc ggtgtcggcg aggaacccga cgatgttcgg cggccacagg acgcgcccgg
     6781 tcccggccac cgtggccccg acgtagcgac cgaactcggt caccgacgag gcgaccgcgg
     6841 tcgggaggcc gtcggtgacg atgatcggct ccgagccgat ccccaggaac gccgtcggcg
     6901 ccgtggtgtc gatcgccgag cccaggtcga cctggtgatc gctgcggact ccgtccgcgg
     6961 tctcggtggt gacggtgaag gttcccccgt cgaccgccga accgccggag ccgatcgcgt
     7021 cgacgactcc gtcggcgctg tcgacgggcc gccccgccac cgccacgacc cgttcgccgg
     7081 ggcgcaggcc cgagcgtgcc acctggccgt cgacgcgcat cgagcccacg acgagctcgg
     7141 atccgttgtc gaccaggtcc aggtcctcac cgacggtgcc gatcacccgg gcccgctgcg
     7201 acaacgtcac cgggaccgtc atctcctgac cgtcgcggtc gatgacgaca tcgacggtcc
     7261 ccggatcgtg gcctccgatc agggatcgga agctgtcgta gtcctcgacg gtgcgcccgt
     7321 cgaagcggac gagccggtcg ccctcgtgca gaccggcggc ctgtgccgcg ctccccggcg
     7381 agatgttccc gatctgccag cggtcaccgt cgggatggcc gatgaaggcg aactggacga
     7441 tcagcaggag gaacgcgacc aggaagtgca tcgtcgagcc ggcgaccgcg accgcgagcc
     7501 ggttgtggaa cggcgcctgt cggtagctgc gggcctcgtc cgcggggtcg acgtcctcga
     7561 gcgaggtcat cccggggatc ttcacgtacg cgccggcggg gatggccttg atgccgtact
     7621 cggtctcacc tcggcggaag gaccagattc gtgggccgaa gccgatgaag aactcggtga
     7681 ccttcatccc ggaccaacgg gcggccaggt agtggccgag ctcgtgcagg aagatcatca
     7741 gcacgatcgc caggatcacg atgacgatcg gcagaccctt ccagacgccc agccccacga
     7801 gaagagccag caggacgccc agacgcagcg cccgggtgag cggcgtgccg agcaccccgg
     7861 ccatctcgtt gcgctcctcg gcgagcgcgg cggggtcgtg gccgggttgg gtcgacggcg
     7921 tgcgtgcgag ggacgactcg gccggcccgc cgtcctggcc cgatccagga tcgtggatgg
     7981 tcacgctgcg acctcccgat cgagcagctc ggtggtgacg gcgcgggctg cggcgtcggc
     8041 ggcgagcacg gcctcgagcg agtcggccgg ggtgccgtcc caacggtcca gggcccgctc
     8101 gaccagctcg gcgatgccgc tccaggcgat gcggccggcc aggaacgcgt cgacggccac
     8161 ctcgttggcg gccgacatcc acgccggggc ggtggcaccg gcgcggcccg ctcgctcggc
     8221 caggtcgagg cagcggaagg tggcccggtc ggggggctcg aaggtgagct cgaaggaccg
     8281 cgtcaggtcc atcgacccga acggcgttcc gatccgatcg gggtaggcca gcgcgtagcc
     8341 gatcgggagg cgcatgtcgg gcatcgacag ctgggcgatc gtggcgccgt cggtgaactc
     8401 ggccatcgag tgcacgatcg actgcgggtg cacgaccacg tcgatgtcgt cgtagtcgac
     8461 gtcgaacagc tcgtgggcct cgatgacctc gagtcccttg ttcatcagcg tggagctgtc
     8521 gatcgtgatc ttcgggccca tgctccaggt ggggtgcgcc agggcgtcct ccctggtcac
     8581 gtcggccagg tccgccgcgg tgcggccccg gaacggcccg cccgacgcgg tcagcacgat
     8641 gcggcgcagg cgccgggtcc cgtcgcccga acgcaggcac tggtgcaccg cgcagtgctc
     8701 gctgtcgacc ggaacgatct ccgctcccgg ggtgtcgcgc acggtgcgaa ccaccgggcc
     8761 cgccgcgatg agcgactcct tgttggccag cgcgagccgg cggcccgagc tcagggcggc
     8821 catcgtcacg ggcagcccgg cgaagccgac caccgcgttg accaccacgt cggccgaccc
     8881 tgccgcggca gcgaaggcgt cgggtccggc gtcgacctcg atgtcggacg cgacgaggct
     8941 ccggagctcg gcggcccgtg accgatccgc gaccacgacg acgtcggggc gtacggaccg
     9001 ggcctgttcg gccagggtca ccacgtcgga gccggcgccg aggaccgaga tgcggaagcg
     9061 gtcgggttcg gcagccacga cgtcgatcgt ctgggtgccg atggatccgg tggagcccaa
     9121 cagggagacg gtggtcagcg agccggtcat ggagcctcca tcgtaccgac acggtccggc
     9181 gtggaacccg gggcctgcct ccagccggga gcctgctcag gagccgtgcg ctccagcgga
     9241 acccgatcct gtgcgacgcc ccgcccctga cgcaccgacg cggtcgggcc cgtcgatcga
     9301 cgggctcagc cgccggtcca ggtgccgaac aggatcgtga cgaagtacgc ggtcggcagg
     9361 acgaacagca gggcgtcgaa gcggtcgagc accccgccgt gtccgggcag gatcgtcccg
     9421 aagtccttga tgccgagatc ccgcttcacg aacgactcgc agaggtcgcc gatgggggcc
     9481 accagggcgc acagcagggc gaacaccacc acccggaaga agtcgcctcc gatcggggcg
     9541 atgccgaaga tgccgacgac gaccacggtg accacgatgg cgacgcccac cccaccggcg
     9601 gttccctcga gggtcttgtt ggggctcgcg gccgacagcg gggtgtgccc gaagtagcgg
     9661 ccgatgaagt aggccccgac gtcgtggctc accgccacca ccaccgcggc gatgagcacg
     9721 ccgatgccga tgttggacga ggcgccggcg tcctcgccga ccttgcccag cccgagcagc
     9781 agcgtggcga aggacccgag gaagccgatc cacatcaccc cgagcagggt gactcccagg
     9841 ttgcgcaccg atccctcgcc cggctgcacg aacaggtacc acagcaggcc cagagccacc
     9901 gtcagcggca ggacgatcgg gtaggcgttc aggcccgagg tgtacgtggc aatcatgaga
     9961 ccgaccgtcg ccaccatgcc gagcagcccg gccggctgga acgagcggcc ctccagggtc
    10021 ccgaagtact cgtaggcggc cacgccgacg accacgcagg ccagggccgt ggtggccagg
    10081 ccacccagct tgaagcacag caggccgacc gccaccaggg cgacgcccac accgacggcg
    10141 gtgaccaggt tgcggtcccc gccgacccca ccgctgtcgg gcggatgctc ctcgccgtcc
    10201 ccggacccgc cgtcgaccgc cgtcatcctc ggacggctgc gggtccgccg cggggcgcgg
    10261 ggctcgctca gggccgagac gtcgtcgtcg ggagcgaagg cctcgatcgg gtcgcggtcc
    10321 gactccgagt cgaagaagta gtcgtcgtca tcgtcgtcgt acccgctgcg gggatcggag
    10381 ccgccgagcg ctcccaggcg gggcgcgtcg tcgagcaggt cgtcgaagtc ggtctcgacg
    10441 tggcgctcac cctcgtcgcg ccagcgcggg gtggcgccga agctgccggt cggctcgtcc
    10501 tcgacgaagt cgtcgccggt caccaccttg gggacctgcc cggtgggggg ctcggtccag
    10561 tgcgggagga cgaacgagtc gtcggacccg gcgaacgggt cgtcggccgg agcggggctg
    10621 cggtcccagt cgggcacgtc gtcgaccggt gcgtaggggt cgggctcgtc cgcggggatg
    10681 gcgcgggcat ggccgaagcc gcgcccttcg tcgtcggccc agcggggctc ctgggacggc
    10741 ggatgcacca cctcgccgct gtcgaaggcg tcgtcgccgg aatcgctggt ggagatgcgg
    10801 accgtcggca gaccctcacc gggcgctgcg gagtcgggtc ggtcgccgta gcgcttctct
    10861 ccggtccgac ggcgccgggc gacgtcggag cgaccggcgg cttcggccgc ctcctgcgcg
    10921 ccgatgatgc ggaccccctc ggaacggggt tcctcgatcc ggcccggatc gtcgtggtcg
    10981 gtcatctccg tcctcctgtc cgcggcacgg gtccgctcag ccctcgagca gctcgacggt
    11041 cttggcctcg agcgcctcgt cgatcgccgc ctcgtgcttc ttggtcagct tgtccagctc
    11101 gccctcggcg cggtggaggt cgtccgagga gatgtcgccg tccttctcga gcgagtcgag
    11161 ctccttgcgg gcgtcgcgtc gggcgccgcg cacggcggtc cgaccgtcct cggccttgga
    11221 cttgaccagg cgcacgtact ccttgcggcg ctcctcggtc agcggaggga acgccaggcg
    11281 gatcgccacg ccgtcgttgg aggggttcaa ccccagcccc gagtcctgga tggcccgttc
    11341 gatgccgccc atcgcgccct tgtcgaaggg ggtgatcacc agcatgcggg cgtccggcac
    11401 cgagaagctc gccagctgct gcagcggggt ggaggcgccg tagtagtcga ccggcagctt
    11461 ctcgaccaga cccgggttgg cgcggccggt gcgcaccccg gcgaactcgt gacgggcgtg
    11521 ggccacggcc ttgtccatgt ggtcgccggc gtcctcgagc acggcttcga tcaactcatc
    11581 gctcacgggc tcagcctccc acaaccggcc ggcctcgggt gcgacaccac tcccccgtcg
    11641 tcgagggaac ggcggcgaac ccggtcgatc cggctcagcg gaccagggtg ccgacgcgct
    11701 cgccggccag gatggcgtgc aggttcgatc ccatcaggtc gaacacgcag atgggcaggt
    11761 cgttgtccat gcacagcgag atggccgtcg agtccatgac cttgagaccg cggttgagga
    11821 cgtcgatgta gctcacctcg gccaggaact cggcgtcggg gtcgagcttg gggtccgcgg
    11881 tgtagacgcc gtcggtgccg ttcttgccca tgaggatcgc gtcggcgtcg atctcgaccg
    11941 cccgcagcgc ggcggtcgtg tcggtggtca tgaacgggtt cccggtgcca ccgacgaaga
    12001 tcacgacccg ccccttctcg aggtggcgga tggcgcgacg ccggatgtag ggctcggcga
    12061 cctgagccat ctggatggcg gtctggaccc gggtgggctg acccagacgc tcgagggtct
    12121 cctgcaacgc cagaccgttg atgatcgtgg cgagcatgcc catgtagtcg gcctgggccc
    12181 ggtccatgcc cgcaccctcg ccggtcatgc cccgccagat gttgccgccc ccgacgacga
    12241 cggcgacgtc cacgtcgaag tcggacttga ccgcgacgat ctccgacgcg atcgcggaga
    12301 cgacctcgcc gtcgatgccg aatccggcct cgccggcgaa cgcctcgccg gagagcttga
    12361 tgagcacccg accccagtgg ggcgtggcca cgatcagtcc ccgatgacgg cctgggcgaa
    12421 gcgcacgatc cgggcgcctc cgaggcgctg ctggaccgtc tccttgtcgc cgaacatgcc
    12481 ctgctcgggc aggacccgct cggcgtacca ggagctcatc cgcccctcga cgatcttggg
    12541 caccgcctgc tcgggcttgc cctccttgag cgtcagctcc tcgaagccgc tgcgggcggt
    12601 ctcggccagg gccgggtcga cctcgtcacg ggacagggct gagggcttgg cgaaggcgat
    12661 gtgcagcgcg acctcgtgtg cggtctcggc gtcggtgccc tcgatctcga tcagcacggc
    12721 gttggtgcca cggccgtcct ggatgtggac gtacgcgtcg agcacgttgc cgtcggcagc
    12781 ctcgaagcgg gcgaccttgc cgatctcgat gttctcccgc ttggcgatct tgaggctgtc
    12841 gaggtcgtcg gagagcgacg cgacggcacc ctcgccgtcg gcgaggacgg cgtcggccag
    12901 ccgctgggtg agctcgacga agtcggcggc cttggccgag aagtcggtct cggacttgag
    12961 ctcgaccagc gaggccgcgt tgccctgggt ggcgatggcc accgcgccct ggttgttctc
    13021 gcggtcctcc agggtcgcga ccttggacag gcccttctgg cgcaggatct gggcggcctt
    13081 ctgacggtcg ccgtcggcct cgaccagagc cttcttggca tccatcatcc cggcgccggt
    13141 ggcgtcgcgg agggacttga cgtctgcagc ggtgatgtcg gccatcagtt ctcctcggtc
    13201 ttgggggtga cggggctggc cgcggcgggc tcgccgtcga gcgcgagctc gtccgccgcg
    13261 acctggttgg cggtcggtgc ctcggaggtg atgtcctcgg cggcagcacc ctcggcgagg
    13321 acggagctct cgtcagccgg cgcctcacga gcagccagca ctcgggcttc gcgctcggcc
    13381 gcggcctgac gggcctcggc ctgggcacgc gcctgctcgg cggccttctc ggcctgctcc
    13441 tcggccgagc gctcacgcgg cgctgattcg gggtgggtct tggagcgcat caggcggcct
    13501 tcgaggacgg cgtccgcgat gacgcgggtc aggaggctgc cggagcggat ggcgtcgtcg
    13561 ttgccgggga tgacgtactg gatgatgtcg ggatcgcagt tggtgtcgac caccgcgacg
    13621 accgggatgc cgagcttgtt ggcctcggtg acggcgatgt cctccttctt ggtgtcgagg
    13681 atgaacaccg cgtcggggag gcgctcgagg tcgcggatgc cgttgaggtt ccgctccagc
    13741 ttgtcgagct cgcgggtgag cagcagcgct tccttcttgg gcatggcctc gaactcaccg
    13801 gagtcccgca tgcgctggta ctccttcatc ttggagaccc gcttggagat cgtctcgaag
    13861 ttggtgagca tgccgcccag ccagcgctgg ttgatgtagg gcatgccgca cttctcggcg
    13921 tagccctgga tcgagtcctg ggcctgcttc ttggtgccga tgaacaggac ggtgccgccg
    13981 ttcgcgacca ggtcccgcac gaagacgtag gacttctcga tgcccgtgag ggtctggtgc
    14041 aggtcgatca ggtagatccc cccgcgctcc ccgtggatga accgcttcat cttggggttc
    14101 cagcggcggg tctggtggcc gaagtgcact ccggcctcga gcagctgctt catgctgacg
    14161 acagcagaca tgacgtgtct ccttcccttc ggttcgacga gacccgggca cgacaccgct
    14221 ccgtgagtca cggggcggcg accgaagagg gcgcccgggc gaggatggat ctggtggatt
    14281 cagctccgac ggctgtcggg ccgaccggcg agtgtagtcg tgtccctccc tcgtccacga
    14341 acgcgctccg cgctccccca cgacgccgtg gcgagtcggg cggatggctg ccccatcatg
    14401 ggggtagcgg gacgagcacg gcgtgcagcg gtgggatctc cgccccgaac aggctcgcgg
    14461 gatcgatgta ctcatcgccg cgccggacgc ccagatgcag tctgagtccg gtcgtcccca
    14521 gaacgtcgcc cgccgcgaca cgctcccccg gggcgactcc gatggtcgcc aggccggtga
    14581 gcgacgagcg cagaccgtcg gggtgctcga cggtcaccac cccgcgtcga gcgaccggac
    14641 cggcgaacac caccacgccg tctccgatcg agcgggccgg cgcaccctcg tcggtggcgt
    14701 actccagccc gcggttgccc ggcccgtacg ggttgatcgg tgcccggaag gggtcgacga
    14761 cgtcgccgtc gaccgggggc cggtacccgc ccggtccggg cgtcggttcc atcgaggccg
    14821 tcccgaccac gggcacggcc gggccgtggg cgggaacggg tgcagccacc gacgggccgg
    14881 acaggagcag cgagacgatc gcgaccagag cggtgcgcac ggggacctcc cggggatcgc
    14941 cctccgggac cggaggacct ggattcgggg gaagtcgagc tgtcgaggtc gcgagatcgg
    15001 gtcacgggcg tcggcccgga ccgctcggac atcgcgtccg gtggcggagg ggcctcagcc
    15061 ggggatgcgg tccatgctgt tgaaccggct gcgcaggtgc aggaccgcct tggtgtggat
    15121 ctggcagacc cgggactcgg tgaccccgag gacccggccg atctcctgca gggtcaggct
    15181 ctcgtagtag tagagcgtga ggacgatctt ctcccgctcc ggcagcccgt tgaccgcctg
    15241 tgccagcagc tggcgggtct cggccacctc gaacgcaccc atcggtccgt gcgaggagcg
    15301 gtccgcgatc gtgtcgccga gcgtgatcga ctctccccgc tcacccgagc cggtgaggat
    15361 ctcgtcgagc gcggccagac cgacgttcgc gatctggttg agcgccgtct ggagctggtc
    15421 gtcggtccag cgcagctcgg acgccagctc ctcgtcggac ggtgcccggt ggtgcttggc
    15481 ctcgagcttg gcgtaggccc gctcgatctg gcgcaccttg gcccgcaccg agcgcggcac
    15541 ccagtcgatc gagcggagct cgtcgaggat cgatcccttg atccggctga tggcgtaggt
    15601 ctcgaacttg tagccccgct ccggctcgaa cttgtcgatg gcgtcgatca ggccgaacat
    15661 gccgtagctg accaggtcgg actgctcgac gttctggggc agacccaccg ccaggcggct
    15721 ggccacgtac ttgaccagcg gcgagtagtg cacgatcagc gcttcgcggg cccgggtcgt
    15781 gcccttgtcc ttgtaggacg accacagccg cgccagctcc tcgtcggtgg tggtgtcctc
    15841 ggtccgggtt cgggtggtca tcaggccctc gggtggctct ctcggaagac ccggctgagc
    15901 cggtccttgc tgacgtgcgt gtagacctgc gtggtcgcca gatcagcgtg accgaggagc
    15961 tcctggacga cgcgcaggtc ggctcctccg tcgaggaggt gggtggcgaa ggtgtggcgc
    16021 agggcgtgcg ggtgcgtggg caccggggac cgccggtcga tcagacgccg gacgtcgcgg
    16081 ggaccgagtc gccgaccccg acggttgtgg aacacagccg cgccatccgt gtcgagcggg
    16141 acggaacctg cgacgtcggt caggaacgac acccggccac cggtgggtgc gagccagtcc
    16201 acgagggcgt cgcgcgccgg cccgctcagc ggcacgatcc gctgcttgga gcccttgccc
    16261 cacacggtga tcaccgaccg ggcgaggtcg acctgatcga cgtcgagtcc gcagagctcc
    16321 gacacccgca ggcccgagcc gtagagcagc tccagcacgg cggtgtccct ggccgtgacg
    16381 accggatcct cggtgtccgg cgactcgtcg agcagccggc ggagctcgtc ggcacgcagc
    16441 accctgggca gtcgggcggc ccccgacggg gcgctgacgc cgacggtcgg atcgaccgac
    16501 accaggcccc ggcgctgaca ccaggtgaag tagcggcgca gcgaggccat ggtccgggcc
    16561 atcgtccgcg gagcccgacc cgaggcgctc aggctcgcga ggtaccgacg gatgcggtcg
    16621 cggtcgacca ggtcgggacg gcggaccggc tcctggggat ccgcctcggc ccatcgggcg
    16681 aaggagctga cgtcacgggc gtagacggcc agagtcgacg gcgccaacgc gacggcggcc
    16741 acgaactcct cgatctccca cgacccgtcc ggcacgctca ccgcccctca cgtcgaaaac
    16801 tgctctccgt agcgtagcga gcgcgctccg tgaacacacg ctcgatcgtc cggacccttc
    16861 ggatccggtt ggcccgaagc ggaccatcga ccctacgggt ccgccgcgtc aacgtcgcgg
    16921 acccccatcg gagcgcgcga cccgttcgac gaacggacct cgacgctcgg cccagccgag
    16981 cgcctccagc cggcccagcg ccaccgacag gcgaccgaga tccagatcca ggtcccggga
    17041 cagcgactcg atgctcaccg gggtgaagcc gagcacggcg aggaccgaag cagcatcgcc
    17101 gtcgggctcg ggccggttct ccacaggtga gttggcgaca ccctcgagcg acagacccag
    17161 ggccgacacg acgtcgtcgg cgccacggca gaccagcgcc ccttccgaga tcagggcgtt
    17221 ggtccccgcc gaggccgggc tgtcgaccgg gccgggaacc gccagcacgg tccgatcccg
    17281 cagctgcgcc tgttccacgg tgagcatcga tccaccccgt tcggccgatt cgacgacgac
    17341 cagcaccgga gccagcgcgg ccaggatccg gttgcggacc gggaaccgcc acggtgccgg
    17401 acgggtgccg ggcggagctt ccgacaggac cgctccgacg tcgacgaccc gccgggcgag
    17461 cgcggcgttg gtcgacggcc ccggcgcgtc cactccccct cccaccaccg caactggtcc
    17521 agcctgggtg cggcccgaca cggaggggtc gacatgggtc tccgctgtcg accccagagc
    17581 ccgatccgac gcggcgacgg caccgttgtg cgcggcggcg tcgataccga gggccaggcc
    17641 cgacacgacg cagaccccgg ccgaggccag atcgagaccg agctcgtgag cgactcgacg
    17701 gccgtagccg gtggcccgtc gcgtgccgat gacggcgact cgagggtggg ccagcgcgtc
    17761 gagatctccc cggtggaaca acagaaccgg cgggtcgagg tcgtcgagca ggcacgccgg
    17821 gtacgacggg gatccaatgg acaccgctcc gatcccccgc gagcgccacc gcgtccagag
    17881 tgcgacggga tccacgctcc gagcctgggc agcccagctg ccgctcgcgt cgcgggcgac
    17941 cggggcgaga gcgttccgca acctcgcgtc gacggcgccg cgacagatcc gttcccacac
    18001 ctcgctcggc gagccgagcg ccagcagcgt tcgcaacgac gcgctgccca tcccgtcgag
    18061 ggtcgcgagc gctcccaggt acgcctcgag cggaacggat cgatcgtcga ggtccgcgtc
    18121 gggcccgtcg ggcgtaccgg acggtcgtcg gggcggggtc atgccgccac ccccagcgcc
    18181 tgggcgtggg tgcgcataga cagggcgacc tgcacccgct cggcgtcgag cggcccgtcc
    18241 agcccgtcga ggtccatgag ggtcagggcc accgctcgga tgcgttgtgc ccccctcatg
    18301 gtcaaccggc cggcggccaa gtggtcgcgc aggaccgcga gcgcctctgc cgacagcggg
    18361 gcggctctct cgagcgcctc gccccggagc tggcggttgg ccgagatgcc gcgttcccgg
    18421 gcccgttcac gagcccgggc gacccggtcg cgcaccacgg cggtgctctc gccggccgat
    18481 cgctgcccga gcacctctga tgccggcggc gggtcgaccc atagcccgag gtcgaagcgg
    18541 tcgagcagcg gacccgacag acgccgctgg tagcgctgga tctgggccgc gctgcatcgg
    18601 cagggcccga acgggccgac cccgcacggg cagggattgg tggccgcgac caggaggaac
    18661 cgggccggca gcgacgcacc gacgtgagcg cgggcgacgc gcaccacgcc cgactcgagc
    18721 ggttggcgca gggcatcgag atgcgcggcg gggaactcac cgagctcgtc gaggaagagg
    18781 acgcccccgg aggccaggga gatctcgccg gggcgcagga ccgtggaacc gccgcctacg
    18841 agggcgacca tggacgcggt gtggtgcgga gcgcgcatgg ggggtctccg cagcagtcgg
    18901 gaggtgtcct cgagcgcccc ggcagccgag tgcaccctcg acacgtccag cgcggcctgg
    18961 tcggtcagat cggggagcag tccggggagg cgttcggcca gcatggtctt gcccccgccg
    19021 ggcgggccga ccatgagcag gtggtggccg ccggctgcgg ccacctcgag ggcgcgacgg
    19081 gcaaccgtct gaccctggac gtcgcggagg tcgggctcgt cgggcggtcc gctgccgtgg
    19141 ggccggcgcg gctcgccgtc gggccatgga aggtcgcccc gcagggcacc caccagctcg
    19201 ctcaacgtcc gggcgcaacg agcgtcggga cggacgagtg cggcctcggc cgcgtcggca
    19261 tgagcgacga ccggccgcag gtcccccgcc gcctcgcaca gcggcagggt tccgatcacc
    19321 gggcgcatcg aaccgtccag gccgagctca ccgatgaagg cgtggccgtc gagcagctcg
    19381 gcatccaact ggccggtggc caccatcagc gccaaggcga tggcgacatc gagaccggca
    19441 cccgccttgc ggagatgcgt gggggcgagg ttgacggtgg tccgggcctg cttgaccacg
    19501 aacttcgacg acagcagggc ggcccgcaca cggtcacgcg actcacggca cgaagcgtcc
    19561 ggaagaccga cgatggcgaa cgacggcaga ccgtcgctga catgggcttc gacccggacc
    19621 gggtacccac gggcaccgat cagggtcgcg gaatgggcga tcgcgagcac tgctgcctcc
    19681 ccggcgacgg agtcgacgct ccgttcgaca cggaacgtgg ggcagcccac gaagctgcga
    19741 tcgcggcagg tacgccgcgg caggaaccct agggccgccc tgtgacaccg ccacccgccg
    19801 gacaccgggg accgcgaccc ggtccgggga acccgactgc gtcaccgacc cgctcctcgt
    19861 cgatcgcccg cgggcgcgat gatctggcca tgggcatgaa cccccaccac cagcatcgtc
    19921 gctccaactg ggactacgtc tacgtgggcg ccgccctcgt ggtggcgctc gccgtgctgg
    19981 cctgggcgtt cttcggctga ggtcggccga gcccatgtcc ggatcgcccc gacgcgggct
    20041 cgaccacacc gccaccgccg agtgcgaggt gcgacgggtc cagccctacg aggcgaccaa
    20101 gctctacctg tgtcccggct gtgcccgcga catcccgcct gggaccggtc acgtcgtggc
    20161 ggtccccctc gatgctccgg acctgcggcg gcactggcac cggggatgct gggacaagcg
    20221 cctgacccgc gccccccgct
//
LOCUS       DAOQOD010000042        20030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_930_length_20030_cov_10.056521, whole genome shotgun sequence.
ACCESSION   DAOQOD010000042 DAOQOD010000000
VERSION     DAOQOD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_20030_cov_10.056521"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PLZ72_06125"
     CDS             <1..1286
                     /locus_tag="PLZ72_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNA35907.1"
                     /translation="DAMARMIRRHWPTGDLTGLPRKDRAGCDYDAYLPDDLVGRRFML
                     DGETAADVSDAEANVARLNASAVTLADTEALARLLLRAEAVGSSHIEGLVVGGRRLLR
                     FEVASELGSDEARDVTADEVLSNIRAMDYAVHTLANEQVTVDGILEMHRLLLEDTDRA
                     QFAGRLREEQNWIGGSSYNPCSAAFIPPPEDDVPRLLADLCTFANGDSLPPIAQAAVA
                     HAQFETIHPFVDGNGRAGRALIHVILRRRGLCPKVVPPISLILATRSSNYIAGLTAFR
                     HVGPADSAAAREGLNHWVATFAVATSAAAHQALAFEDRIEEIKRDWQVRLGRLRRNSA
                     AERLVDLLPGTPIVTSTSAAALLDRSFNAANDAIERFVDAGILTKMTVGRRNRAWEAR
                     EILDAFTDLERGLASPTGDTRAAEPVRRVPARPPS"
     gene            1573..2052
                     /locus_tag="PLZ72_06130"
     CDS             1573..2052
                     /locus_tag="PLZ72_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNA35908.1"
                     /translation="MTAWRDVEAAAPELAARVRELFEARTHKTIATLRADGAPRVSGI
                     ECEFVDGELQFGSMTGARKGADLRRDPRFALHGPTVDPVKGQEAAWPGEAKVAGRAVP
                     AGALTAEPGAADDEVPEGELFVADITEVVITALDEAGTKLVIDSWTPTRGLRRVERA"
     gene            complement(2068..3042)
                     /locus_tag="PLZ72_06135"
     CDS             complement(2068..3042)
                     /locus_tag="PLZ72_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA35909.1"
                     /translation="MTDVIARAEEIADGLLFPAALATDRAELLPVGNLDALADAGLYG
                     LFGPEDLGGWAADLDTAGAAVERIASGCLTTALVWLQHHGLVGNLVLGAGCLRDEWLA
                     DLCSGARRSGIVFTGLLPGPSSLNAVADGDGWVIDGYAPWVSGWGRIDTLQVAARGPD
                     DTVVTVALGELDRPGLTAGRHELAVLDASGTVKLTFDRVGIDADHVIGVAPHRPGETN
                     ALSLRLNGSLALGVARRCCALLGPTALDEQLDEVRNRLDTAGDDDIAEARATASAFAH
                     KAATTLLVHTGSRSIELDDHAQRLAREAMFLMVFGSRPAIRSALLQMV"
     gene            complement(3039..3968)
                     /locus_tag="PLZ72_06140"
     CDS             complement(3039..3968)
                     /locus_tag="PLZ72_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA35910.1"
                     /translation="MDDAGNPADTAVEQQTVIGAGGLPIAVYERNRGAAQTVLLVHGY
                     PDNHSVWDPVAEILAERFHVVTYDVRGAGASGVPDGTRGYRLDRLTDDVAAVIAATLP
                     DDRRVHLVGHDWGSVQGWHFVGDARVAARIASFTSMSGPQLDAVGDFLRRRGTAPPSV
                     ATAAYQAAKSWYVGMFQLPALAPAFWRSPLGRRAWPRVLEQVEGVPAERLPDADALTR
                     TQADGANGVGLYRANVPAAMRSPRPPHTSVPVQLVVAEKDRYVSPAMALCVAPLADEL
                     VVVRVDGGHWLPLTRPERVAELITEHIDRLDRS"
     gene            complement(4021..5373)
                     /locus_tag="PLZ72_06145"
     CDS             complement(4021..5373)
                     /locus_tag="PLZ72_06145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA35911.1"
                     /translation="MKRLGAAAALVVWVTAGIAFGPATGAQPPTTGSTSSTVPDGTGT
                     SSTTTTTADPTTSTTSPDGSSTTSTVPGDTTTTLPEGVTVPPIDPLVPLPPEGDLPTE
                     DEPEDPTAGITPKGPWDGVGTTNPVLAGLRAQVDLTQNRLVAAQTAVAQAEATLAEAQ
                     AARAEVEGAQRAAAERYATVRDRFTRRVAASYVAAGQPQLRLRPGRDMSAEADRVVLP
                     GVITDSDRALADEYLREREKVSERLRTSIEAETAATQTVESARGALAAAQAAATEAEK
                     KLAETPALIAGFVFPVAGPTSFISSYGFCRDGCSRSHQGNDLFARAGTPVVAVEDGWV
                     EKIGTNRLGGLTVWTRGRSGYRYYYAHLRDWAPLEAGQMIAAGTVIGTVGNTGNAITT
                     PPHLHFQIHPGDAPAIDPYPILVRAPRVTLTPEQIAAATGWSVEQATLNQAAAAPPGA
                     "
     gene            complement(5370..6515)
                     /locus_tag="PLZ72_06150"
     CDS             complement(5370..6515)
                     /locus_tag="PLZ72_06150"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNA35912.1"
                     /translation="MPDNDEQLDVEMIDETVEPSARAHTAVQVIGADDPDSLVESGDG
                     AADPDGTDSDESASSFGQDHAINSFLLGESAPGSRREKPWTRVLAMAGYALRDAVHGI
                     RRRPALAASATLTVALCALLAGGAAIARAGVDATMSRWADGVEFVVYLQPGATSADID
                     RVGDELRSSDGVRSVHLVSQQQAYDEYRDLYAEDKTMVEAVTPDLLPASFRVAPADAD
                     PTLIQRITRPLASDPDVYQVVTADETVQDVRDLSGAVSGFGTWLAIVLGVVGVVLSAT
                     MIRSSIAARQREIAMMRSVGATRTYIAAPVAIEGVLIGVAGAGVAAVTLTLALTQAAG
                     SSSSVVTSLLPPSGEARTVILVVFAATLAVCTAVSVLTTASTLRRSR"
     assembly_gap    6812..6911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6912..7910
                     /locus_tag="PLZ72_06155"
     CDS             <6912..7910
                     /locus_tag="PLZ72_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNA35913.1"
                     /translation="QADTAQAGTAPAGNLIDRVSDRLDRWQRSVSPLAVVHAVLKKYG
                     EDRGGQLAMILTYRGFFAAFPLLLAFVNVVGLLVQDNEELREELINSTLGDVPIIGTE
                     VLKADVGGSVAVVVGSVLLSLWAGLGLLEALQELLNTTWGIPVYDRPNWFVRRARSIP
                     AVLLLGGCLVLSGSRAWFDLPPVLDDVVSVLMPVTAGALCYLGLHWLLCPRKVPFVAQ
                     LPGALFVGLSWYVLLTLAEWYVGRFVVRSSDTYGVFVVVFGLLSWAYLLGTLYLYGNE
                     LSSVLYEHRWPRSLTGRNLTDADRDAFARVSEREVRVRGTDIAIDVPTDPAPPPER"
     gene            complement(7948..9957)
                     /locus_tag="PLZ72_06160"
     CDS             complement(7948..9957)
                     /locus_tag="PLZ72_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNA35914.1"
                     /translation="MLIGRPLASDEADHQRISKTIGLAVFSSDAISSTAYATGEILIV
                     LVGAAGMVQANGEPTTNLLKPIAVLVVILLALVANSYRETIHAYPDGGGAYVVARENL
                     GETPSLVAGASLLVDYVLTVAVSIAAGVLALYSLFPALRPYRVELCLLFLIILTLGNL
                     RGVKESGKIFSVPTYIYIAILAVFITWATVRHLGGNLPVFDPTTAENVESIKKIQEDH
                     GPYIGGISMFIFARAFASGAVALSGVEAISNGIPAFRKPTSKNAAITLGWMAAILGST
                     FFMISIIGSWMRVIPNESQSALAIMGEQVFGGDNPLFWVLQLATTAILILAANTAYAD
                     FPRLAAIIGKDGYLPRQFANRGDRLVFSNGILILAGAAAILLIVFNGDVTLLIPLYAV
                     GVFTSFTISQTGMVRHHFKERQKGWHLSAVNSTLGAIATFIVAMVVIVSKFAIGAWII
                     VVLIPCIVVAFRSVKRHYNHVARSLRVDPDFRVPARVLRHGVVVLVGGVNQSSLGAIR
                     YARTVGAEDMVAVTVAIDADEAARVRDSWQRFGVDIPLEVIESPFRDLTHTVVDYLDE
                     LDTRWHHDYLTVVIPEFVLPHWWQGVFHNQSALALKVALKFRKDTVVVNVPFLLPDDD
                     GAETATEAAAALGLDFSHEGGLAGVGPDEQPTIDRIEPASRREHD"
     gene            complement(10120..10620)
                     /locus_tag="PLZ72_06165"
     CDS             complement(10120..10620)
                     /locus_tag="PLZ72_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNA35915.1"
                     /translation="MATIPDEEWYEVVVEIPQGSRNKYEIDHDTHEVWLDRHLFAATV
                     YPAEYGFFPDTLAEDGDPLDALVLLEDPTFPGCHVRTRPVGQLEMTDEAGRDLKVLCV
                     PAGDPRWAHIQDVADVDPFLLNEIHHFFRVYKDLEPGKGSDIGDWTGRADALASIAAA
                     RAAHPG"
     gene            complement(10651..11229)
                     /locus_tag="PLZ72_06170"
     CDS             complement(10651..11229)
                     /locus_tag="PLZ72_06170"
                     /inference="COORDINATES: protein motif:HMM:NF039596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35916.1"
                     /translation="MRMPAAQRRTQLLDTAVHVFAERGYHAASMNDVAEAAGVTKPVL
                     YQHFSSKRDLFVELLTAIGDELRETIDKATADAAGPRQQVEQGMRAYFHYVDARTDCF
                     RVLFGAGARRDPEFASFARSVEESIAESIAELIVVDGQPAAHRDVLAHGIVGMTEAAS
                     RHWLAHDRTPDVDTLASQLSRLAWSGMRGLDG"
     gene            11391..12455
                     /locus_tag="PLZ72_06175"
     CDS             11391..12455
                     /locus_tag="PLZ72_06175"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HNA35917.1"
                     /translation="MSESPPPVAPVPAVAGPVLERLRCAPFHRDQHVDPIGNAAGYDG
                     YLVVDVAPPWDREVTDQEPLRSIVGGQSSAAVSADGSRWRPLARVPDPGLVARGLRRV
                     TAHRATVADLDGLELTGPFERREWVLPAERVVALGRAVLGLEPLDGDDPIGDDEISGA
                     LVAPEVAGSDLLICTHGRRDQCCGSLGTTVHELLGQLWAGSAAPARRQRISHTGGHRF
                     APTSITLPDGYLWSHLDAEAADRLVRRVDPPEAFALNCRGCSLFPSPARQIADRAGLI
                     EVGWEWADAPRGFQLAAFDRATMATTVRVTAVLAGGAVRSFDVTAVPDRQVPTPTCGV
                     IDAPEYSTDTVWRVDRVEPV"
     gene            complement(12474..12752)
                     /locus_tag="PLZ72_06180"
     CDS             complement(12474..12752)
                     /locus_tag="PLZ72_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35918.1"
                     /translation="MDRDGSQAQDPVLAKRARILHWTEVGQRVAYLCFALSVVVFVIA
                     FIAQFPPWTVSVIVGLIVVGSVVFIPAVILGHAAKAADREDRGETSHY"
     gene            12903..13646
                     /locus_tag="PLZ72_06185"
     CDS             12903..13646
                     /locus_tag="PLZ72_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35919.1"
                     /translation="MARAGRERLLIENCGEEHALSADRTLTFGRSADVVVDINPYLHR
                     VLGRFRHDGRSWWLDNVGRSIPLTLLSIEGLSSATVGPGSSTPILAVESAVTFSAGPA
                     SYELVVTNERAERLVDLGSVAGDGPLVTLEWGHVELNADQVDMLRVICGPRLDRPADQ
                     WADIPTNRAAAAELGWSLAKYNRKLDHVCDKLARVGVRGLRGDLGLSAMDRRRTLVDH
                     VVRSGLLAALAVPDDGPEVPQQSSAGRAG"
     gene            complement(13660..>14301)
                     /locus_tag="PLZ72_06190"
     CDS             complement(13660..>14301)
                     /locus_tag="PLZ72_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35920.1"
                     /translation="ASVDAETLPPASGWRVPVGIRHDDLGVAVLDLRRSGALVLGPPR
                     SGRSTALAMVVSRLDPTVCGSVLTVDGHRPDGAVDALSELLERITAGGTGTEGTTSTA
                     STIVAVDDLPELLDGPDGVAIDAVLDRLLRIGPDVPLRVLATAEADAASRCYADSFRR
                     LRSTRTGLLLRPDPDLHPPLLHTSLPLHDELVPTAGRGWLVDPDGVVAVQLAR"
     assembly_gap    14302..14401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14402..17960)
                     /locus_tag="PLZ72_06195"
     CDS             complement(<14402..17960)
                     /locus_tag="PLZ72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNA35921.1"
                     /translation="MTYRVLHDGAERDVRVDLADDTTVGEVARAIAEHLGDTTAPNPT
                     SEAPGSVRTLTAVPGTAATGEGSTAVDPDRLAVDAAPRCAATVRVVTASEHPVEPPWW
                     SPVVLTRLVAAVEPDPGDPGAGDARLAYGTTTLGPLRLRVGSALSAHAIGSTDRVEVD
                     GTPLRGGVRLGHGSIVRIGDSTFTVRIEGPLRPPDAEGPWRDHGRIPAVVDPDQPRSV
                     ELPTPPSADRLPGFPVLSAAVPLLMGAAMWVATRSLAVTGFVLFSVAFVVASGIEVRR
                     EHRRERRFREESFRSDLNRAVEHARTRRAEQLERAERHLPSGAEVAAWVHADRRRVWE
                     RHPDQPDALRVRIGTGDGPAMDPVVVAAGGRADLRVELDAAVEELSVQELPIGVDLGS
                     SDLALCGPGDEPAALARAVLLQLAATLGPEHLEVVLDVAPERAPAWEWLRWLPHRVSD
                     GDPTVDPASQSEPGSSPRRVVTLVDRSGWGPGPVPGAHLRRAGTIWVASSVTEVPDGV
                     ATVVTTTPTAAVVRRHDGAPATLRPDGVALEECEPVARALAPLRAPSGDDSVPATVSL
                     RSVLATPGLLDDDAAVLDRWVQSRTRPGSLRAPIGATGGGVVDIDLCSDGPHGLVAGT
                     TGSGKSELLRTLVVSLALHHPPDRLTFLLVDYKGGAAFRGISELPHVVGLVTDLGQAE
                     ARRALVSLRAEVRRRERVVASAGVTDMVEVPAADASPALLVVVDEFATLAAELPDLLD
                     GLLDVAQRGRSLGVHLLLATQRPGGVVTDAIRANLSIRLALRVADEEDSRDVIDGVDA
                     AHLPAEHPGRAVLRLGPGRRSTLQVASTTGAATPERRVVVHDLHPGPTTAVTSARPGT
                     GPTDPATASPTELDAAVDRCRSAAASEPGRAAPRRPWVEPLPEHLERSALERPSRPGA
                     LVIGLADLPAEQRRTTVEVDLERRGGVLVMGAPRSGRSGALRTIAAASVSDPRTPTTV
                     HAIDNGRSLDGIERTAGAGGTVDVPVGADDAEATLRLLRSLRRRCGTAGTGTADPGSR
                     GRVVLLVDGIGAFVERHERVNRGEAVDLLTALAGDGPASGVHVVVTAGRAAEVPPAIL
                     AVLDQRVLLRCATPDDAAVLDVVDPDGLARELASPDLPAGRGLFDGRLVQVAAPPILL
                     AGPDGDPGRPRSVAPRRARRLAASVDAETLPPASGWRVPV"
     gene            complement(17997..18263)
                     /locus_tag="PLZ72_06200"
     CDS             complement(17997..18263)
                     /locus_tag="PLZ72_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35922.1"
                     /translation="MAKLGLDPEAMAALQKQLQADAGTINSLTRKLDGLLKSAWWEGS
                     DATKFRSEWDGNHKAQLTRVAAALEATAQAVAANVSQQRQASQS"
     gene            18424..19098
                     /locus_tag="PLZ72_06205"
     CDS             18424..19098
                     /locus_tag="PLZ72_06205"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HNA35923.1"
                     /translation="MAIVPTLLAIGIGVGLGLYWGGRLSNLWEWVPPLWEALVGGVVL
                     TVLVDLVPWSGPVMTFLSIVATAALLGFAVVNVRIGGMVLVVAGLGLNLLVTVLNWGM
                     PVSTSALVSAGIITEAQADSVVLNGGRTADGSILGFLGDVIPLPWGQVISIGDVLILV
                     GLALVVASILRRYEVGRPSGHHGRGGSGDYSSALAALGRGPAPRRGPGLHPSRLGGPE
                     GGRRRR"
     gene            19110..19517
                     /locus_tag="PLZ72_06210"
     CDS             19110..19517
                     /locus_tag="PLZ72_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35924.1"
                     /translation="MVDNTAFQVLLLIHILAAVVAFGGNFVQPMLQRANTDDAALAKV
                     NKLIQLPALVVLFLAGIGLILTSKVQDVAVFKFDQMWISVAFLVVIVAIVLQYLIARA
                     YENGSTSAVPGLTGGLHLCLIVGLYLMIWKPGL"
     gene            19564..>20030
                     /locus_tag="PLZ72_06215"
     CDS             19564..>20030
                     /locus_tag="PLZ72_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA35925.1"
                     /translation="MSIVSYSRDGAVARLTISRPERRNALNHTALDELHDGVRRAAAD
                     QVRVLVLTGDADQFCAGADLKELEDLAFTRALRAALDDLAALPFPTVAAISGACMGLG
                     MQLALSCDLRLATPDARFAVPVAKLGLMVDHWTIQRLALLAGPSTARWMVLTAR"
BASE COUNT         2686 a         6947 c         7345 g         2852 t
ORIGIN      
        1 gagacgcgat ggcgcgcatg atccgccggc attggcccac cggagatctg accggccttc
       61 ctcgcaagga ccgcgccgga tgcgactacg acgcgtacct gccggacgac ctcgttggcc
      121 gccggttcat gctcgacggc gagacggctg ctgacgtctc ggacgccgag gcgaacgtgg
      181 ctcgcctgaa cgcgtcggcg gtgacgctgg cggacaccga agcgctggct cgcctcctcc
      241 tccgcgccga ggcggtcggc tcatctcaca tcgagggtct cgtggtcggt ggccgccggt
      301 tgctgcgctt cgaggtcgcg tccgaactcg gctccgatga agcccgtgac gtcacggcgg
      361 acgaggtgct gtcgaacatc cgtgcgatgg actacgccgt gcacacgttg gcgaacgagc
      421 aggtcaccgt cgacggcatc ctggagatgc accgacttct gctcgaggac accgacagag
      481 cccagttcgc cggacgactg cgtgaggagc agaactggat cggaggctcg agctacaacc
      541 cgtgctctgc agcattcatc ccgccgcccg aggacgacgt gccccgactg ctcgccgacc
      601 tgtgcacgtt cgcgaacggg gactcgttgc ccccgatcgc gcaggccgcc gtcgcccacg
      661 cccagttcga gaccatccac ccgttcgtgg acggcaacgg ccgagcgggt cgagctctca
      721 tccacgtcat ccttcgccgc cgcgggttgt gcccgaaggt cgtgccgccg atcagcctca
      781 tcctcgccac tcgatcctcg aactacatcg ctggactgac cgcctttcgc cacgtcggcc
      841 cggccgacag tgcagcggct cgggagggcc tgaaccactg ggtcgcaacg ttcgcggttg
      901 cgacctctgc ggccgcacac caggcgcttg ctttcgagga caggatcgag gagatcaagc
      961 gggactggca ggtgcgtctg ggaagactcc gccgcaactc tgcagcggaa cgtctcgtcg
     1021 acctccttcc tgggacgccc atcgtcacct cgaccagcgc cgccgccctc ctcgaccgct
     1081 ccttcaacgc ggcgaacgac gccatcgaac gattcgtcga cgccggcatc ctgaccaaga
     1141 tgaccgtcgg gcgcaggaac cgagcctggg aggcgcggga gatcctcgac gccttcaccg
     1201 acctggaacg agggctggcc tcaccgaccg gagacacccg cgctgctgag ccggtccgac
     1261 gtgtcccggc tcgtcctccg agctgagcgc ctccggtcgt tcctcatggg cgtccgaagg
     1321 ctccggatcg actagtggcc agcctggttc ggcacgtcct gacccggcga acttgtagct
     1381 ctcggtcgct gcggtggcgt gtgggctaca agttcgctgg tccgtgtcga acttgtagct
     1441 ctcggtcgct gtggtggcgt gtgggctcca agttcgctgg ttgctgctcg acgtgttccc
     1501 cggtgaccgc gacgccgtgt cgccctaggt tgtcgacatg gaccagttgc gcggagatcc
     1561 cgggaccagc cgatgacggc gtggcgtgac gtcgaggccg ctgcccccga gctcgccgcc
     1621 cgggtccggg agctgttcga ggcgcggacc cacaagacga tcgccacgct gcgcgccgac
     1681 ggcgccccgc gcgtctcggg catcgagtgc gaattcgtcg acggcgagct gcagttcggg
     1741 tcgatgaccg gggctcgcaa gggggccgac ctgcgccgcg acccgcgctt cgcgctgcac
     1801 ggcccgaccg tggacccggt caagggtcag gaggccgcct ggcccggtga ggccaaggtc
     1861 gccggacggg cggtgcccgc cggggcgttg accgccgaac cgggagcggc cgacgacgag
     1921 gtgcccgagg gcgagctgtt cgtcgcggac atcaccgagg tcgtgatcac cgcgctcgac
     1981 gaggccggga cgaagctcgt gatcgactcg tggacaccta cccgggggct gcggcgcgtc
     2041 gaacgggcct gaggtccgac cgcggcgtca gaccatctgc agcagggcgc tgcggatggc
     2101 gggacgggag ccgaacacca tcaggaacat cgcctcgcgg gccagtcgct gcgcgtggtc
     2161 gtcgagttcg atcgagcggc ttccggtgtg gaccagcagc gtggtcgccg ccttgtgggc
     2221 gaacgcagag gcggtggcgc gggcctcggc gatgtcgtcg tcgccggcgg tgtcgagacg
     2281 gttgcgcacc tcgtcgagct gttcgtcgag cgcggtcgga cccagcagcg cgcagcaccg
     2341 tcgcgcgacc cccaacgcca gcgacccgtt gagccgcagg ctgagggcgt tggtctcgcc
     2401 cggccggtgc ggggccacgc cgatcacgtg gtccgcgtcg atgccgaccc ggtcgaaggt
     2461 gagcttcacg gtcccgctgg cgtcgagcac ggcgagctcg tggcgaccgg cggtcagtcc
     2521 cggccggtcc agctcaccga gagcgacggt gacgacggtg tcgtcgggcc cgcgggccgc
     2581 gacctgaagc gtgtcgatgc gaccccagcc cgacacccag ggggcgtatc cgtcgatcac
     2641 ccacccgtcg ccgtcggcaa cggcgttgag gctggacggt ccgggcagca gtccggtgaa
     2701 cacgatgccg gaacgtcggg ccccggagca caggtccgcc agccactcgt cgcgcaggca
     2761 gccggcgccg agcaccaggt tgccgaccag gccgtggtgt tgcagccaca ccaacgcggt
     2821 ggtcaggcat cccgaggcga tccgttcgac ggcggccccg gcggtgtcga ggtcggcggc
     2881 ccagccgccg aggtcctcgg ggccgaacag cccgtacagc ccggcgtccg cgagggcgtc
     2941 caggttcccg accggcagca actcggcccg gtcggtggcc agggcggcgg ggaacagcag
     3001 cccgtcggcg atctcctcgg cgcgggcgat gacgtcggtc acgatctgtc cagtcggtcg
     3061 atgtgctcgg tgatcagctc ggccacccgc tccggtcggg tcaacggcag ccagtgtccg
     3121 ccgtcgacgc gcacgaccac cagctcgtcg gcgagcggag cgacgcacag cgccatcgcc
     3181 gggctcacgt agcggtcctt ctcggccacg acgagttgca ccggcaccga cgtgtgcggc
     3241 ggtcgtgggg agcgcatcgc cgcgggcacg ttggcccggt acaggccgac tccgttggcg
     3301 ccgtcggcct gggtgcgggt gagcgcgtcg gcgtcgggga gccgctcggc gggcactccc
     3361 tcgacctgtt cgaggacacg gggccaggcc cgtcggccga gcggcgaccg ccagaacgcg
     3421 ggcgccaacg cgggcagctg gaacatgccg acgtaccagg acttggccgc ctggtacgcc
     3481 gcggtcgcca ccgacggggg agcggtcccg cggcggcgca ggaagtcgcc gaccgcgtcg
     3541 agctgcggtc ccgacatcga tgtgaacgag gcgatgcgtg ccgccacccg ggcgtcgccc
     3601 acgaagtgcc agccctgcac cgagccccag tcgtggccga ccaggtggac ccgccggtca
     3661 tcggggagcg tcgccgcgat caccgcggcg acgtcgtcgg tcagccggtc gaggcggtag
     3721 ccgcgggtgc cgtcgggaac gcccgacgcg cccgcgccgc gcacgtcgta ggtcaccacg
     3781 tggaagcgct cggcgaggat ctccgccacc gggtcccaga ccgagtggtt gtcgggatag
     3841 ccgtgcacca gcagcaccgt ctgggcggcg ccccggttcc gctcgtagac cgcgatcggc
     3901 aacccgccgg caccgatcac ggtctgctgc tcgacggccg tgtcggcggg gttccccgcg
     3961 tcgtccatcc cgagagcctt ccccatccgg accggacccg gccagccccg gtcggcggcg
     4021 tcaggctccg ggaggtgcgg cggcggcctg gttgagcgtc gcctgctcga ccgaccagcc
     4081 cgtggcggcg gcgatctgct cgggggtgag ggtgacccgc ggggcgcgca ccaggatcgg
     4141 gtacgggtcg atcgccggtg cgtcgcccgg gtggatctgg aagtgcaggt gcgggggagt
     4201 ggtgatggcg ttgccggtgt tgccgaccgt gccgatcacc gttccggccg cgatcatctg
     4261 gccggcctcg agtggggccc agtcgcgcag gtgggcgtag tagtagcggt agccgctgcg
     4321 accccgggtc cacaccgtga gcccgcccag gcggttggtg ccgatcttct ccacccagcc
     4381 gtcctcgacc gcgacgaccg gggtgccggc ccgggcgaac aggtcgttgc cctgatgcga
     4441 tcggctgcaa ccgtcgcggc agaacccgta gctcgagatg aagctggtcg gtcccgcgac
     4501 cgggaacacg aacccggcga tcagcgccgg ggtctcggcc aacttcttct cggcttcggt
     4561 cgcggcggcc tgggccgcgg ccagggcgcc gcgggcggac tcgacggtct gggtggccgc
     4621 ggtctcggcc tcgatcgagg tccgcagccg ttcggagacc ttctcgcgct ctcgcaggta
     4681 ctcgtcggcc aacgcccggt ccgagtcggt gatcacgccg ggcaggacca cccggtcggc
     4741 ctcggccgac atgtcccgcc cgggccgcag tctcagctgg ggttggccgg cggcgacgta
     4801 ggaggcggcg acgcggcggg tgaagcggtc ccggaccgtg gcgtagcgct ccgccgcggc
     4861 gcgctgggcg ccctcgacct cggcgcgggc ggcctgggcc tcggccaggg tggcttcggc
     4921 ctgggccacc gcggtctggg cggcgaccag gcggttctgg gtcaggtcga cctgggcccg
     4981 cagacctgcc aacaccgggt tggtggtgcc gactccgtcc cagggtccct tcggggtgat
     5041 cccggcggtc ggatcttcgg gctcgtcctc ggtgggcagg tcgccctcgg gcggcagggg
     5101 gacgagcggg tcgatcggcg ggacggtcac gccctcgggc agcgtcgtgg tcgtgtcacc
     5161 cgggacggtg ctggtggtgc tcgatccgtc gggcgacgtg gtcgacgtcg tcggatccgc
     5221 cgtggtcgtg gtggtgctcg aggtgccggt gccgtcgggg acggtgctcg acgtcgagcc
     5281 ggtcgtcggg ggctgggcgc ccgtcgcggg accgaacgcg atgccggcgg tcacccacac
     5341 gacgagcgcg gcggccgcgc cgagccgctt caccgggacc gcctcaacgt gctggcggtg
     5401 gtcagcaccg acaccgcggt gcacaccgcg agggtcgccg cgaacacgac gaggatcaca
     5461 gtgcgcgcct cgcccgacgg cggcaggagc gatgtcacca ccgagctgct cgacccggcg
     5521 gcctgggtga gggcgagcgt cagggtcacc gcggcgacac cggcgccggc aacaccgatg
     5581 agcacgcctt cgatggcgac cggggcggcg atgtaggtcc gggtggcgcc gaccgaccgc
     5641 atcatggcga tctcgcgctg acgggcggcg atcgacgagc ggatcatcgt cgccgacagc
     5701 acgacgccga ccacgccgag gacgatcgcc agccaggtcc cgaaccccga caccgcaccg
     5761 gagaggtccc gtacgtcctg cacggtctcg tccgcggtga cgacctggta cacgtcgggg
     5821 tcagacgcca gcggtcgggt gatccgctgg atgagtgtcg gatcggcgtc ggcgggagcg
     5881 acccggaacg atgccggcag caggtcgggt gtgaccgcct cgaccatcgt cttgtcctcg
     5941 gcgtacaggt cgcggtactc gtcgtaggcc tgctgttggg agacgaggtg caccgaccgg
     6001 accccgtcgg aggatcggag ctcgtcgccg actcggtcga tgtcggccga ggtggcgccg
     6061 ggctggaggt agacgacgaa ctcgaccccg tcggcccagc gcgacatggt ggcgtcgact
     6121 ccggcgcggg cgatggcggc gccgccggcc agcagggcgc acagcgcgac ggtgagcgtg
     6181 gccgatgccg ccagggcggg tcggcgacgg atcccgtgca ccgcgtcgcg cagcgcgtag
     6241 ccggccatgg ccagcacccg ggtccacggc ttctcccgcc gcgaccccgg ggcggactcg
     6301 ccgagcagga aggagttgat ggcgtggtcc tgaccgaagg agctggccga ctcgtcggag
     6361 tcggtgccat cggggtcggc ggccccatcg cccgactcga ccaggctgtc ggggtcgtcg
     6421 gctccgatca cctgaaccgc ggtgtgggcg cgcgccgacg gctccacggt ctcgtcgatc
     6481 atctcgacgt cgagttgctc gtcgttgtca ggcacgcgcg aaaggtctcc caggaacctg
     6541 gacctgcagg ccctcgacga gcccggtcca gagtgtgtcg gccgatccgg cccgaacccg
     6601 tagcggaatc ttccgcctcg cccccgcgtc gggctgccca cccgcccgga acggccgggt
     6661 gggcacggcc cacgagggta caggagccct gccgctgcga acagggcggg ccggtgaacc
     6721 acggttgacc caccggcgcc ggagtaccgt ccgacgatgg cgagcgaccg cgacggcggc
     6781 gacacagcac aggccgacac agcgcaggcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn ncaggccgac acagcgcagg ccggcacagc accggccggc aacctgatcg
     6961 accgggtctc ggaccgcctc gaccgctggc agcgctcggt ctcaccgttg gcggtggtcc
     7021 acgccgtgct caagaagtac ggcgaggacc gggggggtca gctcgccatg atcctcacct
     7081 accggggctt cttcgctgcc ttcccgctgc tgctcgcctt cgtcaacgtc gtcggactgc
     7141 tggtgcagga caacgaggag ctgcgcgagg agttgatcaa ctcgaccctc ggcgacgtcc
     7201 cgatcatcgg caccgaggtc ctgaaggccg acgtcggcgg cagcgtcgcg gtggtggtcg
     7261 gctcggtgct cctgtcgctg tgggcggggc tgggcctgct cgaggcgttg caggagctgc
     7321 tcaacacgac gtgggggatc ccggtctacg accggcccaa ctggttcgtc cggcgggcgc
     7381 ggtcgatccc ggcggtcctg ctgctcgggg gctgcctggt gctgtcgggg tcgcgggcct
     7441 ggttcgatct gccgccggtg ctcgacgacg tggtgtcggt cctgatgccg gtgaccgcgg
     7501 gagccctgtg ctacctgggg ctgcactggc tcctgtgccc gcgcaaggtg ccgttcgtcg
     7561 cccagctccc cggcgcgctg ttcgtcgggt tgtcctggta cgtgctgttg accctcgccg
     7621 agtggtacgt cggacgtttc gtcgtccgca gctcggacac ctacggcgtc ttcgtcgtcg
     7681 tgttcggcct gctcagctgg gcgtacctgc tcggcaccct gtacctgtac ggcaacgagc
     7741 tgagctcggt cctctacgag caccggtggc cgcgcagcct gaccggccgc aacctgaccg
     7801 acgcggatcg agatgcgttc gcccgggtgt ccgagcgcga ggtccgggtg cgcggcaccg
     7861 acatcgccat cgacgttccc accgacccgg ccccgccgcc ggagcgctga cgtcgaccgg
     7921 atccgtcggc gacgtcggcg tccgtgttca gtcgtgctcg cgacgcgatg cgggttcgat
     7981 ccggtcgatc gtcggttgct cgtcgggtcc gacgcccgcc agtccgccct cgtgggagaa
     8041 gtccaggccg agtgcagcag cggcctcggt ggcggtctcg gccccgtcgt cgtcgggcag
     8101 caggaacggc acgttcacca ccaccgtgtc cttccggaac ttcagcgcca ccttcagcgc
     8161 cagcgccgac tggttgtgga agacgccctg ccaccagtgg gggagcacga actcggggat
     8221 cacgacggtg aggtagtcgt ggtgccagcg ggtgtcgagc tcgtccaggt agtcgacgac
     8281 tgtgtgggtg aggtcgcgga acggcgactc gatgacctcg agggggatgt ccactccgaa
     8341 gcgttgccag gagtcgcgca cccgggcggc ctcgtcggcg tcgatggcga cggtcaccgc
     8401 caccatgtcc tcggcgccca cggtgcgggc gtagcggatg gcgcccagcg acgactggtt
     8461 cacgccgccg accagcacga ccaccccgtg gcgcagcacc ctggccggga ccctgaagtc
     8521 ggggtcgacc ctcaggctcc gggccacgtg gttgtagtgg cgcttgacgc tgcggaacgc
     8581 caccacgatg caggggatca gcaccacgat gatccaggcg ccgatcgcga acttggagac
     8641 gatcaccacc atcgccacga tgaaggtggc gatggccccg agcgtcgagt tgaccgcgga
     8701 caggtgccag cccttctgac gctccttgaa atggtggcgc accatgccgg tctgggagat
     8761 ggtgaagctg gtgaacacgc cgaccgcgta cagcgggatc agcagcgtca cgtcgccgtt
     8821 gaacacgatc agcaggatcg ccgcagcgcc ggccaggatc aggatgccgt tggagaacac
     8881 cagccggtcg ccgcggttgg cgaactggcg gggcaggtag ccgtccttgc cgatgatcgc
     8941 cgccaggcgg gggaagtcgg cgtacgcggt gttggccgcc aggatcagga tggcggtggt
     9001 cgccagctgc agcacccaga acagcgggtt gtcaccgccg aagacctgtt cgcccatgat
     9061 cgccagcgcg gactgcgact cgttggggat gacccgcatc cagctgccga tgatgctgat
     9121 catgaagaag gtggagccga ggatcgccgc catccagccg agggtgatcg ccgcgttctt
     9181 cgacgtcggc ttgcggaacg ccgggatgcc gttcgagatc gcctcgacgc cggacagcgc
     9241 gacggcaccc gacgcgaacg cccgggcgaa gatgaacatc gagatgccgc cgatgtaggg
     9301 gccgtggtcc tcctggatct tcttgatcga ctcgacgttc tcggcggtgg tcggatcgaa
     9361 caccggcagg ttgccgccca ggtggcggac cgtcgcccag gtgatgaaga cggccaggat
     9421 cgcgatgtag atgtaggtcg ggaccgagaa gatcttgccg gactccttga cgccgcggag
     9481 gttgccgagg gtcaggatga tcaggaacag caggcagagt tcgacccggt aggggcgcag
     9541 ggcggggaac agcgagtaga gcgccagcac gcccgctgcg atcgacaccg ccacggtgag
     9601 cacgtagtcg accagcagcg acgccccggc gaccagcgac ggggtctcgc ccaggttctc
     9661 gcgggccacg acgtaggcgc cgcccccgtc ggggtaggcg tggatcgtct cccggtacga
     9721 gttcgccacc agtgccagca ggatgaccac cagcacagcg atcggcttga gcaggttggt
     9781 ggtgggttcg ccgttggcct gcaccatccc cgccgcgccg accagcacga tgaggatctc
     9841 gccggtcgcg tacgcggtcg aggagatcgc gtccgaggag aacaccgcca ggccgatggt
     9901 cttggagatg cgctggtggt cggcctcgtc gctggcgagc gggcgaccga tgagcacacg
     9961 tttgagcgtc gatgccatgc ggcggcgaac ttacggtcgc acccgggggc gagggaagcc
    10021 cagagtcccg gaaatgtccg gacgcgcgag atgttcacat tccgggtgac cgccgtcacc
    10081 gccggtgggg ggccgtgggg ggtcgagtgc gcgccggtct cagccgggat gagcggcgcg
    10141 ggccgccgcg atcgacgcca gggcgtcggc ccggccggtc cagtccccga tgtcggagcc
    10201 cttgcccggt tccaggtcct tgtagacgcg gaagaagtgg tggatctcgt tgagcaggaa
    10261 cgggtcgacg tcggcgacgt cctggatgtg ggcccagcgg gggtcgccgg ccgggacgca
    10321 caggaccttg aggtcgcgtc ccgcttcgtc ggtcatctcc aactggccga ccgggcgggt
    10381 gcggacgtgg cagccgggga acgtcgggtc ctcgagcaac accagggcgt cgagggggtc
    10441 gccgtcctcg gcgagggtgt cggggaagaa gccgtactcg gcggggtaga cggtggcggc
    10501 gaagaggtgg cggtcgagcc agacctcgtg ggtgtcgtgg tcgatctcgt acttgttgcg
    10561 ggacccctgg gggatctcca ccacgacctc gtaccactcc tcgtcgggga tcgtcgccac
    10621 cgctgccctg ccttccggtt cgggtcgggc tcagccgtcg agcccgcgca tacccgacca
    10681 tgccagccgc gacagctggg aggccagcgt gtcgacgtcg ggtgtgcggt cgtgcgcgag
    10741 ccagtgtcgg ctggccgcct cggtcatgcc gacgatgccg tgggcgagca cgtctcggtg
    10801 cgcggcgggt tggccgtcga cgacgatcag ctccgcgatc gactcggcga tcgactcctc
    10861 gaccgatcgg gcgaacgacg cgaactcggg gtcgcggcgc gctccggcgc cgaacaggac
    10921 gcggaagcag tcggtgcggg cgtcgacgta gtggaagtag gcccgcatgc cctgctcgac
    10981 ctgctggcgc ggtccggccg catccgcagt ggccttgtcg atcgtctcgc gcagctcgtc
    11041 gccgatcgcg gtgagcagct cgacgaacag gtcccgcttg gagctgaagt gctggtacag
    11101 gacgggcttg gtcacccccg cggcttcggc cacgtcgttc atcgacgccg cgtggtagcc
    11161 acgttccgcg aacacgtgca ccgccgtgtc gagcagctgg gtgcgccgct gggcggccgg
    11221 catcctcatc gctgcgaccg gacccgggcc tggcggggcc cggagtcggg agtggcggac
    11281 cggggcatgc ggaccatggt accgcccatg ttaccgcgtg gtaacaagcg ggtgcgggga
    11341 cccggtcggt ggctcgtcgg cgccggcccc ggcctcggta gcctgcccgg gtgtccgagt
    11401 cccctccgcc ggtcgctccg gtccccgccg tcgcgggccc ggtcctggag cgactgcgct
    11461 gcgccccgtt ccaccgcgac cagcacgtcg acccgatcgg caacgcggcg ggctacgacg
    11521 gctacctggt ggtcgacgtc gctccgccgt gggaccggga ggtcaccgac caggagccac
    11581 ttcgctcgat cgtcggcggg cagagctccg ccgcggtctc cgctgacggt tcgcggtggc
    11641 gtccgctcgc gcgggttccg gatcccgggc tggtcgcccg cgggttgcgc cgggtcaccg
    11701 cgcaccgggc gacggtggcc gatctcgacg gcctggagct gacggggccg ttcgaacgtc
    11761 gtgaatgggt ccttcccgcc gagcgggtgg tggctctcgg acgggccgtg ctcggtctcg
    11821 aaccgctcga cggcgacgac ccgatcggcg acgacgagat cagcggtgca ctggtggcgc
    11881 ccgaggtcgc cggctccgac ctgctgatat gcacccacgg acgccgcgac cagtgctgcg
    11941 gcagcctcgg caccaccgtg cacgagctgc tcggacagct gtgggccggc tcggccgcac
    12001 cggcccgacg ccaacggatc tcccacaccg gaggccaccg gttcgctccg acctcgatca
    12061 ccctgcccga cggctacctg tggtcccacc tcgacgccga ggccgcggac cgtctcgtgc
    12121 gacgggtcga ccctccggag gccttcgccc tcaactgccg gggctgctcg ctgttcccga
    12181 gtccggcccg tcagatcgcg gaccgggccg ggctgatcga ggtcggctgg gagtgggccg
    12241 acgccccccg cgggttccag ctcgcagcgt tcgaccgcgc caccatggcc accaccgtga
    12301 gggtgacggc cgtgctggcg gggggtgcgg tccggagctt cgacgtcacc gcggtgccgg
    12361 accgacaggt gccgaccccc acctgcgggg tgatcgacgc tccggagtac tcgaccgaca
    12421 cggtgtggcg cgtcgatcgg gtcgagccgg tctgagcggt cgcggttgct cgctcagtag
    12481 tgcgacgtct cgccgcggtc ctcgcggtca gcggccttgg cggcgtggcc gaggatcacc
    12541 gccgggatga acacgaccga gccgacgacg atcagcccga cgatgacgct caccgtccac
    12601 ggcgggaact gggcgatgaa ggcgatcacg aacacgacga ccgacaaggc gaagcagagg
    12661 taggcgacgc gctggcccac ctcggtccag tgcaggatgc gcgcccgctt cgccagcacg
    12721 gggtcctggg cctggctgcc gtcccgatcc atgcgatcag cctgcccgat ccgaggcggt
    12781 cgcggcgatt cccacctccc tcgggcggtg tgcctcaaat caactgaaat gacatgagat
    12841 ggccttgaat ggtatccatg tcgaaccaca gcacaggccg cgggacgacg gaggcgaggg
    12901 ccatggcgag agcggggcga gaacgccttc tgatcgagaa ctgcggtgag gagcacgccc
    12961 tttccgcgga ccgaacgctc accttcggcc gctccgccga cgtggtcgtc gacatcaacc
    13021 cgtacctgca ccgcgtgctc gggcgcttcc gccacgacgg ccgctcctgg tggctcgaca
    13081 acgtcggtcg gtccatcccc ctgacgctgc tgtcgatcga aggcctgtcg tcggcgacgg
    13141 tgggacccgg gtcgtcgacc ccgatcctgg cggtcgagtc cgcggtgacc ttcagcgccg
    13201 gtcccgccag ctacgagctg gtggtcacca acgagcgggc cgagcggctg gtcgatctcg
    13261 gctccgtcgc cggcgacgga cccctggtca ccctggagtg gggtcacgtc gagctcaatg
    13321 cggatcaggt cgacatgctc cgcgtgatct gcggcccgag gctggaccgc ccggcggacc
    13381 agtgggccga catccccacc aatcgggcgg cggcggccga gctcggctgg agcctggcca
    13441 agtacaaccg caagctcgac cacgtctgcg acaagctcgc ccgggtcggc gtgcgcggcc
    13501 tgcgtggcga tctcggcctc agcgccatgg accgccgtcg gactctggtc gaccacgtcg
    13561 tgcgcagcgg cctgctcgca gccctggcgg tgcctgacga cggcccggag gtgccgcagc
    13621 agagctctgc cggtcgggcc ggctgaaggg acgctccgct cagcgggcca gctgcacggc
    13681 gaccacgccg tcggggtcga ccaaccatcc ccggcccgcg gtcggcacga gctcgtcgtg
    13741 caacggcagc gaggtgtgca gcagcggagg atgcaggtcc gggtcgggcc gcaggagcag
    13801 acctgtccgc gtcgatcgca gccgtcggaa gctgtcggcg tagcagcggg acgccgcgtc
    13861 cgcctcggcg gtggcgagca cgcgcagggg cacgtccggt ccgatccgca gcagtcggtc
    13921 cagcacggcg tcgatggcga cgccgtcggg cccgtccaac agctcgggca ggtcgtcgac
    13981 ggcgacgatc gtcgacgccg tcgacgtcgt gccctcggtg cctgtgcccc cggcggtgat
    14041 ccgttcgagg agttcgctca gggcgtcgac cgcaccgtcg ggacggtggc cgtccacggt
    14101 gagcacggag ccgcagaccg tcgggtcgag tcggctgacg accatggcca gcgcggtgct
    14161 acggcccgac cggggaggcc cgagcaccag ggcaccgctg cggcgcaggt cgaggaccgc
    14221 cacgccgagg tcgtcgtggc ggatcccgac cgggaccctc catcccgacg ccggcggcaa
    14281 cgtctcggcg tcgaccgagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 ncgaccggga ccctccatcc cgacgccggc ggcaacgtct cggcgtcgac cgaggcggcc
    14461 aggcgacggg cgcgccgcgg ggcgaccgac cgggggcgtc ccgggtcgcc gtcgggcccg
    14521 gccagcagga tcggtggcgc agccacctga accaggcgcc cgtcgaacag cccccgaccc
    14581 gccggcaggt cggggctcgc cagttcgcgg gccaggccgt cggggtcgac gacgtcgagc
    14641 actgccgcgt cgtcgggggt ggcgcagcgc agcagcaccc tctggtcgag gacggccagg
    14701 atcgccggcg gcacctcggc ggcgcgcccg gcggtcacca ccacgtgcac gcccgaggcg
    14761 ggtccgtcgc cggcgagcgc ggtgagcagg tccaccgcct cgccccggtt cacccgttcg
    14821 tgacgctcga cgaaggcgcc gatcccgtcg acgagcagga cgacgcgccc gcgcgagccg
    14881 gggtccgctg tgcccgtccc cgcggtgccg caccggcggc gcaacgagcg caggagccgg
    14941 agggtggcct cggcgtcgtc cgctccgacg ggcacgtcga cggtccctcc ggccccggcg
    15001 gtccgttcga tcccgtcgag cgagcggccg ttgtcgatcg cgtggacggt cgtcggggtc
    15061 ctcggatcgc tgaccgaggc cgccgcgatg gtgcgcaggg ctccggagcg ccccgagcgg
    15121 ggagcgccca tgaccagcac cccgccccgg cgctccaggt cgacctcgac ggtcgtccgg
    15181 cgctgctcgg cgggcaggtc ggcgagtccg atcaccaggg ctccgggacg tgatggccgc
    15241 tccagcgccg accgctcgag gtgctcgggc agcggttcca cccacggtcg tcgaggagcg
    15301 gctcgcccgg gttccgacgc ggcggcggag cggcagcggt cgaccgccgc gtcgagctcg
    15361 gtgggggaag ccgtcgccgg atcggtcggt ccggtcccgg gtcgggccga tgtcaccgcg
    15421 gtggtcggtc ccgggtgcag gtcgtgcacc accacgcgcc tctcgggcgt ggcagctccg
    15481 gtcgtgctgg cgacctgcag ggtcgatcgt cgaccgggac ccaggcgcag gacggcccgg
    15541 ccggggtgct ccgccgggag gtgggcggcg tcgacgccgt cgatgacgtc acggctgtcc
    15601 tcctcgtcgg cgaccctcag ggcgaggcgg atcgacaggt tggcccggat tgcgtcggtg
    15661 accaccccgc ccgggcgctg ggtggcgagc agcaggtgga cccccaggct gcggccgcgc
    15721 tgggccacgt cgagcagccc gtcgaggagg tcggggagct cggcggccag ggtggcgaac
    15781 tcgtcgacga cgaccagcag cgcgggggac gcgtcggcgg cgggcacctc gaccatgtcg
    15841 gtcacacccg cagatgccac cacccgctcc cggcggcgga cctcggcacg cagcgacacg
    15901 agcgcccgac gggcctcggc ctgtcccagg tcggtgacca ggccgaccac gtggggcagc
    15961 tccgagatcc cccggaacgc ggcgccgccc ttgtagtcga ccagcaggaa cgtcaggcgg
    16021 tccggcgggt ggtgaagcgc cagggagacc acgagggtcc gcaggagctc gctcttgccc
    16081 gacccggtcg tgccggccac caggccgtgg ggcccgtcgg agcacagatc gatgtcgacg
    16141 acgccgccgc cggtcgcacc gatcggcgcc cgcagcgacc ccggacgggt ccggctctgc
    16201 acccaccggt cgagcaccgc ggcgtcgtcg tcgaggagcc ccggcgtggc gaggacggag
    16261 cgcaacgaca ccgtcgcggg caccgagtcg tcccctgacg gggcacgcag cggggccagc
    16321 gcccgagcca ccggttcgca ctcctccaac gcgacgccgt cgggtcgcag cgtcgccggc
    16381 gcgccgtcgt gccgtcggac gacggcagcg gtgggtgtgg tcgtgacgac cgtggcgacg
    16441 ccgtcgggaa cctcggtgac cgaggacgcg acccagatcg tcccggcgcg acggaggtgc
    16501 gctccgggca ccgggccggg accccagccg gaccgatcga ccagcgtgac gacccggcga
    16561 ggcgaggacc ccggctcgct ctgcgacgcc gggtcgaccg tcggatcgcc gtcgctcacg
    16621 cggtggggga gccaacgcag ccactcccac gccggtgccc gttcgggggc gacgtcgagg
    16681 accacctcga ggtgctcggg gccgagggtg gcggccaact gcaacaggac cgctcgggcc
    16741 agcgccgcgg gttcgtcgcc gggaccgcac agcgccaggt ccgagctgcc gaggtcgacg
    16801 ccgatcggca gctcctgcac cgacagctcc tcgactgctg cgtcgagctc gacgcgcagg
    16861 tcggcgcggc cgccggcggc cacgaccacc gggtccatcg cggggccgtc gccggtgccg
    16921 atgcggaccc gcagggcgtc gggctggtcc ggatggcgtt cccacacccg gcgacgatcg
    16981 gcgtgcaccc acgcggcgac ctcagcgccg ctgggcaggt ggcgctcggc ccgttcgagc
    17041 tgttcggctc gacgggtgcg ggcgtgctcg acggcgcggt tcaagtcgct gcggaagctc
    17101 tcctcgcgga agcgccgctc gcgtcggtgc tcccgacgga cctcgatgcc ggaggcgacc
    17161 acgaacgcca cgctgaacag cacgaagccg gtcaccgcca ggctgcgggt cgcgacccac
    17221 atcgcagcgc ccatcagcag cggaacggcc gcggacagga ccgggaagcc gggcagtcga
    17281 tccgcggagg gcggtgtggg cagctcgacc gaccggggtt ggtcggggtc gaccaccgcc
    17341 gggatccgtc cgtggtcgcg ccatgggccc tctgcgtcgg gtggccggag cgggccctcg
    17401 atccggaccg tgaacgtgct gtcgccgatc cgcacgatgc tgccgtggcc cagccggact
    17461 ccacctcgca gcggcgtccc gtcgacctcg acccggtcgg tcgaaccgat ggcgtgggcg
    17521 ctgagggccg agcccacccg cagccgcagc ggaccgaggg tcgtggttcc gtaggccaac
    17581 cgggcgtctc cggctccggg gtcacccgga tccggctcga ccgcagcgac cagacgggtc
    17641 aggacgaccg gtgaccacca cggcggttcc accgggtgct cggacgccgt gacgacgcgg
    17701 accgtcgccg cgcaccgggg ggcggcgtcg accgcgaggc ggtcggggtc gaccgcggtc
    17761 gacccctcgc cggtggccgc cgtccccggg accgcggtga gggtgcggac gctgcccgga
    17821 gcttcgctcg tcgggttcgg ggcggtcgtg tccccgaggt gttcggcgat ggcccgggcg
    17881 acctcgccga cggtggtgtc gtcggcgagg tcgacccgga cgtcgcgctc ggcgccgtcg
    17941 tggaggaccc ggtaggtcac gtgtcgcatc gtctcggtcc tcgcgggtcg acgtgctcag
    18001 gactgcgagg cctggcgctg ctggctgacg ttggcggcca cggcctgggc ggtcgcctcg
    18061 agcgcggcgg cgacccgggt gagctgcgcc ttgtggttgc cgtcccactc gctgcggaac
    18121 ttggtggcgt ccgaaccctc ccaccaggcc gacttgagca ggccgtcgag cttgcgggtc
    18181 aacgagttga tcgtcccggc gtcggcctgc agctgcttct gcagagccgc catcgcttcc
    18241 gggtcgagtc cgagcttggc catggtgagc acctcctgtg cgggccgccc ggtccgtccg
    18301 ggcgactccg aggagggaac aacggcgaga ccgctcctgt catcggggac cgcgacccgt
    18361 tgacgggaac ccgaacctgt cctaggggtg ctccgggagc cggccgacgg ctagtttccc
    18421 gcggtggcca tcgtcccgac cctcctcgcc atcgggatcg gcgtcggcct cgggctgtac
    18481 tggggtgggc ggctgtcgaa cctgtgggag tgggtgccgc cgctgtggga ggcactcgtc
    18541 ggcggggtcg tcctcaccgt cctggtcgac ctcgtgccct ggtccggtcc ggtgatgacg
    18601 ttcctgtcga tcgtggcgac cgccgcgctg ctggggttcg cggtggtcaa cgtccgcatc
    18661 ggcgggatgg tgctggtggt cgcggggctg ggactcaacc tgctggtcac ggtcctcaac
    18721 tggggcatgc cggtcagcac ctcggcgctg gtgtcggccg ggatcatcac cgaggcccag
    18781 gcggactccg tggtcctgaa cggcggccgc acggccgacg ggtcgatcct cggattcctg
    18841 ggcgacgtga tcccgctgcc gtggggtcag gtcatctcga tcggcgacgt cctgatcctg
    18901 gtcgggctcg cgctggtcgt cgccagcatc ctgcggcgct acgaggtggg gcggccgagc
    18961 ggtcaccacg gtcggggcgg atcgggggac tacagctccg ctctggccgc cctgggtcgt
    19021 ggcccggctc cgcgcagggg accgggcctg cacccgtcgc ggctcggagg tcccgagggc
    19081 ggtcgccgtc gccggtaggc tgcgccgcca tggtcgacaa caccgcgttc caggtactgc
    19141 tgctcatcca catcctggcg gcggtcgtcg ccttcggcgg caacttcgtg cagccgatgc
    19201 tgcagcgggc caacaccgac gacgccgcgc tggccaaggt gaacaagctg atccagctgc
    19261 cggctctggt ggtgctgttc ctggccggca tcgggctgat cctgaccagc aaggtccagg
    19321 acgtcgcggt gttcaagttc gatcagatgt ggatctcggt cgccttcctg gtggtgatcg
    19381 tcgccatcgt tctgcagtac ctgatcgccc gggcctacga gaacggatcg acctcggcgg
    19441 ttccgggtct gaccggtggg ctgcacctgt gcctcatcgt cggcctctac ctgatgatct
    19501 ggaagccggg cctctgacgc ggtccgcagc ccggacctcg ccctgcagac cggacaccgc
    19561 gccgtgagca tcgtcagcta ctcccgggac ggcgcggtgg cgcgcctgac catctcccgt
    19621 cctgagcggc gtaacgcgct caaccacacc gccctcgacg agctgcacga cggcgtgcgc
    19681 cgggcggccg cggaccaggt gcgggtgctg gtcctcaccg gtgacgcgga tcagttctgc
    19741 gccggggccg acctcaagga gctcgaggac ctggcgttca cccgggcgct gcgtgcggct
    19801 ctcgacgacc tggccgcact gccgttcccg accgtcgccg ccatctccgg ggcgtgcatg
    19861 ggcctgggga tgcagttggc cctgtcgtgc gacctccgac tggccacgcc cgacgcccgc
    19921 ttcgcggtcc cggtggccaa gttgggcctg atggtcgacc actggacgat ccagcgcctg
    19981 gcgctgctgg ccgggccgtc gacggcgcgg tggatggtgc tgaccgcccg
//
LOCUS       DAOQOD010000043        19821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_947_length_19821_cov_13.982546, whole genome shotgun sequence.
ACCESSION   DAOQOD010000043 DAOQOD010000000
VERSION     DAOQOD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_19821_cov_13.982546"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..643)
                     /locus_tag="PLZ72_06220"
     CDS             complement(26..643)
                     /locus_tag="PLZ72_06220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA35926.1"
                     /translation="MTEDPASFWDDRYRSTPPRDLSWFQAVATPSIELIAELDVDPLS
                     GVIDVGGGASPLVDGLVDRGFTDLTVLDVSGAALAMARERLGSAPVSWVVGDVTTWRP
                     DRTWDLWHDRAVFHFMTDPEQRAGYLRSLDAAVAPGATIVVATFAPDGPERCSGLVVD
                     RYDAEGLVAALTERIPARLLMARRQTHTTPWGATQAFTWVALRRS"
     gene            complement(640..1683)
                     /locus_tag="PLZ72_06225"
     CDS             complement(640..1683)
                     /locus_tag="PLZ72_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA35927.1"
                     /translation="MLSVVAGSGTTDADTNRLLLGVLRRSTGRADLAFGDPPSPISGG
                     FDAGLLRFRLESPPPDLDRELVARIVARSPLGRFEAEVQRRVADAGIPTPVVRLAVDD
                     PDGLGGFVIVMDHVPGRPPLAGLGPAAVLGNLPRLTRQLPDQLASVAGRLHAVDPAPV
                     ERALRSEGTEVPITAVELAEHLSAAAEDRGRGDLVGACGDLLRHRPDGGTRAVTHGDL
                     HPFNLIDGPDGTVLIDWTLAAIAHPAFTLAFTQLVLAHPPIALPTPARTALGPIARAM
                     ARRFTTTYRTLCPDADDHLDPTQLDWHRRLHALRVLVEMSAWDGDAPAGHPWLILAPI
                     ARQILDRPRGPSS"
     gene            1772..2719
                     /locus_tag="PLZ72_06230"
     CDS             1772..2719
                     /locus_tag="PLZ72_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35928.1"
                     /translation="MRRRPEPEILVPTRAPSRRSVLRAGALLGGLGAAGALVGCGDDG
                     SSAGGSTTTAAGGGATTGALIASFPQSTPYIAAGSPARMPYLIADREGVPLRSIDGPV
                     RFTVSRDGTPVGEPVEVTPRSDGVPRAYLPLAFTFAEPGIYDVSAEYGGSTLEGSVQV
                     VDRSKITTPMVGDRLPAVASPTTANAMGVDPLCSRDPVCPYHSVSLDAAVASGKPAIL
                     VVSTPAFCQTAVCGPLLDHVMEAVGEPGGINVIHSEVYANPTAVDDLAKATVAPVPKA
                     FDMAFEPALFVADASGVVVSRGDIIVDRGELAEMLAPIR"
     gene            complement(2733..4364)
                     /locus_tag="PLZ72_06235"
     CDS             complement(2733..4364)
                     /locus_tag="PLZ72_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA35929.1"
                     /translation="MKKTALIALAIAQFVMVLDQSVMNVSISQLVADFDTTVPTIQAV
                     ITLYCLVMAMLMLTGGKIGDIIGRRRAFTIGLVIYAVGSATTAVAPTVAVLALGWSVL
                     EGIGAALVLPAMAALIAGNFEGTARKQAYAVIGGVAGAGIAVGPIVGGWATTELSWRV
                     VFAGEVVLVAIILALTPKVADALRDGPAPRLDVVGAVLSATGIGAVVLGILQSSTWGF
                     LTPKDSPVEPLGFSLAVFVVIGGALLLWAFVVWSRHREAQGRDPLVHLRLFEVLPLRS
                     GLVGLLTQNLVLMGVFFVVPLYLQLVIGLDALDTGLRMLPISITMFLFSAIGSRLSAR
                     VSVRTIVRTGLVVSTLAIVVLIATVDAQLNDLGFAVSMALLGVGMGLIVSQLGNVVQS
                     SVDASGRGEAGGLQYTGQQLGSSVGVALIGAIVLAGLTNVFVRTIDADERIAPEVAQQ
                     VSTAAGNGVDFVASDDLAAAATDAGIDEQSADALVDDYEQAQLRALKTGLLVTGVLAL
                     LSLMSTRHLPATVTAGDDDREDDPVGDDPVGADPL"
     gene            4552..4944
                     /locus_tag="PLZ72_06240"
     CDS             4552..4944
                     /locus_tag="PLZ72_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNA35930.1"
                     /translation="MILADVNLGEILWTTLVIFFMVMYFMILFSILGDLFRDHDTSGW
                     GKAAWVLFLVIAPFLTALIYLIARGNGMAKRSMAAQADAQKQFNEYVQSVAGSGSPTE
                     QIAQAKQLLDSGAIDQAEFDRLKAKALG"
     gene            5045..6715
                     /locus_tag="PLZ72_06245"
     CDS             5045..6715
                     /locus_tag="PLZ72_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA35931.1"
                     /translation="MLNPRTYERGWLRFDLVAGIVLAAILVPQGMAYAELAGLPPVTG
                     LYTTIACLVAYAIFGPSRVLVLGPDSSVSPLILASITPLVVVGDPGSAIVLAGMLAVL
                     VGLIEIGLGVAKLGFVADLLSSEVRVGYMNGLAVTIIVGQLPKLFGFSTDADGFVDEL
                     KAFWNGLDQTNRTTLITGLAVLLVLLVLPRITTRIPAVLVAIVGVTVVSALLGLADEG
                     VKTVGTLPQGFPTPSLPWTSASDALALMVGAVGITMVSLTDTIATASSFAARRGDEVD
                     ANAEMIGVGASNIAAGFFQGFAVSVSSSRTAVADQSGARTQLTGLVGAGLVALLLLVF
                     NGLLADLPQTALAAVVIVAALSLADLPALARFARLRRSSLVLSLVASAGVIFLGVLQG
                     IVVAIVLAILLFFRRNWWPHGTVLAEVPELGGWHAVSVHPEGRQVDGIVVFRWEAPLF
                     FANAGQFRDQVRALVRDSSPAYVVLQCEAVTDIDVTAAEVLKDLDEELNAKGVHLAFV
                     ELRDRLADLIVRYDLDATLQAEHFYASVEEAVAALASERDGGPAGAGS"
     gene            6717..>7356
                     /locus_tag="PLZ72_06250"
     CDS             6717..>7356
                     /locus_tag="PLZ72_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA35932.1"
                     /translation="MSDDSTTAPEDGSGQSQEGSRWRRRKRPGPRRAEGMPTGPIEVR
                     RAVIDAAAQLFCRDGVAEVTLRDVAQRARVNLGLISRYVGSRDDLVRATFADLSDKLT
                     AEVRADPTAARGFESDSVMVCWTRILSHLVVVDPETAVELGRAPMEDLVAAAQDVYGQ
                     SEPAARLRVAQLMGSAMGWRLFEPFLMEAAGLDPDSIDDIRAELLGVHRRLAA"
     assembly_gap    7357..7456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7466..8839)
                     /locus_tag="PLZ72_06255"
     CDS             complement(7466..8839)
                     /locus_tag="PLZ72_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNA35933.1"
                     /translation="MAKKSGRRSERPTDPRERAESGRAVRSVVPRASLGDWTPAPDRD
                     PLGVLSAQDKVRDQDLVPLRHSRMGVSPFTFYRGAAAVMAADLAPMPRTGLDVQLCGD
                     AHLANFGVFASPDRAMVFDLNDFDETHPGPFEWDLRRLVASFEVAARDRSFDDPQRVQ
                     IVADSVLSYVSAMRQYSTMSNLEVWYDRLTVDDIAQRWAARASDDVLRRFRRNVDKAR
                     SKDRIRAFDKLVEVTDGTPRFRSDPPVLVPVRELVTGAEHAALMKAVDESLKLYRRTL
                     APDRRLLMDRYRFVDLARKVVGVGSVGTRCWVALLIGNDETDPLFLQVKEANASVLEA
                     HLGASRYGQHGQRVVEGQRLIQSSPDVFLGWDQVRGPDGVARDFYFRQLWDWKGSADL
                     ERMPAEAMAMYAAICGRCLARAHARTGDSVALAAYLGKGRTMTASLVEFARRYADQNE
                     ADHRSFS"
     gene            8950..10239
                     /locus_tag="PLZ72_06260"
     CDS             8950..10239
                     /locus_tag="PLZ72_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNA35934.1"
                     /translation="MNEERRGADALTLPDPRRRAAAAVAILTALAALAALAVGLVRSF
                     PQGPSLVLMVVVAGGLGLDGLRRRGASRRIELVVAAVVLAAVVVLAVVWRTGGAALVA
                     AVLLALGGVAARIAFAARVVRPAAPRPSRPVVIWNPRSGGGKAEKANLGAEAAARGIE
                     AVELRPGDDLVQLVEDAVARGADALAAAGGDGTQALVATIAAREGLPFACIPAGTRNH
                     FALDLGVDRDDVVGALDALVDGGERVVDLAEVNGRVFVNNASLGVYAEAVQRDSYRGA
                     KTSTLLNTVPDAFGKDGPDHPTLRWTGPDGRPGPPVAALLVSNNPYRLGTIVGSGTRP
                     DMDGATLGVVSLGTRLQRWTAPEFVVDSDRPVPVGIDGEALVMDPPLVFRSRPRALRV
                     RIAQAHPGASPSSDAPVSAWAALPALWRIVTGRPGVG"
     gene            10744..19242
                     /locus_tag="PLZ72_06265"
     CDS             10744..19242
                     /locus_tag="PLZ72_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35935.1"
                     /translation="MTDRFSLEPRFYKAGTELLPIDTNLSADGSRTAKTVSAGGKGLA
                     ATANWFSTKLGNLSQGVTVGVGADALTMTPVGAVSLPDPTSLSNVAVYPEVWPGVDLR
                     YRVTPTGLKEEMVLKNPSVASSYSFSLTGATTTGPVVAGKGDVAAQFDGSLGLSGSLG
                     ERVMFAPPVVTGDGDGRIFTDAKPTLTGSGSSVSVAVDPAWLAAQPASEFPIVVDPTI
                     TGRAGTGWASFAAGGGSVGDSYVGNSCVNPPTCSGYLWRSMANFSHGELYSNFVQSAT
                     LHMAAGAVGSPSGTLGLVGWASSADYDGAATYINGQSSVFGIGAGTSFADVDITETMR
                     DMAELDPGNYGLLGFAFYQTSGYYSLHRLGFSTIDVTYESDGHVSNLSPASGSQTAHT
                     RRPTLTATVGNAGGATTVNVRFQVACPGQSTIDSNWLAASPGTPYSWALPSDMPWNAE
                     CRWYAGGRNSSYEDWSPGYSITAVSQPAPAPALDRTMDGSIVSTPTLSLSAPGTLTDP
                     ESDTVYLDYSIATGSDGVTGRVVRSPLLALNTTFSAPTSSLPDGIYYATVRSVDSTGA
                     ASAWVKPIRFRVVQRLGVQAKMAYDSTGPFNVNLASGNLVVAASGPSFSTLGGDAGVN
                     FTYNSQAAVPKGVRAAFWDDTNADGVLQSSGSPKDAKRAERIDPVASYNWNDTAGPIP
                     GTVNPNSFIAEVTGSYKNTTGSAQNVSFQAVPVDSTGAGNKVKVQVKGGAAAGGPETS
                     AWNTATTAVTVPANDTVSVRVEYAKLAAVASSGFRIVFSEGSGWADVPADRWMQYAPV
                     LPGGWSSTGPGLGTLGYSRLSPGENQVTLYDDSGAAHVWRSNGDSFTPPPDEDGVLRR
                     QPNGFTYTNAAGTSVTVSSGFEFVDDDGYQYLFDDTGQIVSVVAPADAVNPGALRYAY
                     ARLYTSGPMRMTQIKDPFGRTVDLTYKDPSTANCPTDGSSSQPPSYTLCLVTYTGFTR
                     NSTGTAPTTNLYYDTAGNLAAVVNPGDTGTGVVRPRTDFAYDAAGRIVGVRDSYTNDL
                     IASSVFTATNVPSSDPHWWTVTYGSGGTDGMVTQLTAPEPSSGGTRPQQTYTWNLTST
                     YPGAKSATRVAVAGSVNASGWTQHVDIDTAGRPVQSIDQGGVAIETFWDPDLDKQDRV
                     VKTIDHHYLANPTTGLQTTTIYDAADRPTDTYGPGTPSEFIGQTSATAPHATTAYDEG
                     IPGLAAKWFNNTSASGPAALYSTSSGTESWGSGAPSNAAFPGAAIGNDFSGTLTGQAT
                     FASPTALGVRADAGRLFVDDIKVGDTFNGAYRAAVTTDQPSNYWRLDDTTGSTTATDI
                     ASGVTGTYIGAPTLGSVGALNQGGSADPDTSITVGGTGVSGETARGVNVTAPSGTDAL
                     NLSGDMAIEAWVNPSTTTGYQMIVDRSTSTGTTAVYELRLNAGRLEWCQVNNGVITVV
                     TAATALPVNAWSHVAATRISTPTGLVIKLYVNGVDVTPAGGPFNVATASLPTDTTRIG
                     NRTDGWVFSGKLDDVAIYSHGLSAARVATHFAAATNATAAELSTANAWTQTIRDDGPS
                     QWWRLGETTGTTAVDASSEGSSPGVYSSPTLGATGAIVGDPDTAVTFNGTSGGVTLPD
                     DVISSRSLTIEMWFKATGAGALYGQRNATSTKYAPILYVGTDNKLYGGLWGPGYGAAN
                     LVSTGSVVGNGWHHVALTSNGVSQTLYLDGAQIGDRSGVPDVLGMPIGSIGSAWTNGW
                     TATNNAWFVFNGTIDDVAVYSYPLSGSRVKTHYDALSLGEFSGTKRVRVDYQDLTGNA
                     ALQVVQGRPGSSTAVSAGVLSPRYGLVTSTVDPDGKKTSSEYTNPELGLKTADVADPA
                     GLNLRTVYTYEGASAANQQFRRQLTRSLPGITGTATTPVTYGYWAQGSGATNTADNPC
                     TTGTTETINQGGAAKTRTAVDPDGAASATAQTSETRYHGDGLIAATKRNAETGWTCFT
                     YDGRGRTLTQTIPAFGSSPQRVVTTNYKADPDGAGAAPATAEWTSVTDDAASTVATSP
                     ILTKTDWLGRTISYTDALGRTTTTAYDQAGRVTTTTGPTGYGTVTQAYDSNGRPTTSS
                     VNSQPIATTTYNTSSGVATSTTYPSGAGNAGNGVTVTNGFDTRGRATSVTWTGPESTA
                     IASNTVSRSAAGRIVDEATDGTIGATGDANTTGPNYTYDGAARLTDAYAPGHHYTYNY
                     GTPSGSDCPSTSPVYATLIANKNSNRTSVTDQPLPSGTATTTKSCYDAADRLTSTTAG
                     SIGTNVTYDTRGRTTTLGTDSYAYDQTDRHSATTANASAARNALLAVGGPAALTSRDT
                     WLQQRLIDAGWTVTIGDDDTVTASSASGKQLIVLSESVTQAGIGTKFTSVAVPLISAE
                     PFLYDELGMTGTGTNQGGTSGATETQVAVTAAGVQSSLGAGLSAGNATIASSGVDLSW
                     GKPAATATVAATIASDATKATLFGYRTGAAMVTGTAPAPRVGWTHYTGSPTPLNSTAV
                     ALFNAAIGWAAPASATTSVTYVRDVTDRIIARKVNGNLNACFSYTAGGDTPDQTLAPS
                     GSSCSTTVTETVSGLPGGATLTSRGNSTTDVWSLSNIHGDTLAITNGNGVKQGTTLTY
                     NPDGQALGDNPDNSTGAFDNGWLGRHQRPLERQGNLLQTIEMGARPYSVTTGRFLEID
                     PVEGGTANDYTYVTDPLNSFDLDGMCGVFGNPFKKCSKDQKGQRGFLGGVFSKGARGV
                     RVIMTLPASQLGITWAKMHRGKCSWDGTHWMSVCTGMRGGYGRGGTTVGSVYMSGRTP
                     DARMMRHESKHANQYAWFGGGAMFPVVYGIQEWTSGGGKHNTFERQAGLKDGCYVANR
                     PFCR"
BASE COUNT         3164 a         6939 c         6626 g         2992 t
ORIGIN      
        1 cgatcatgat actggcctgc gccggtcagc tccgtcgtag ggctacccac gtgaacgcct
       61 gggtggcacc ccacggggtg gtgtgggtct ggcgtctggc catgagcaac cgggcgggga
      121 tccgctcggt cagcgcggcg accaggccct cggcgtcgta gcggtcgacg acgagacccg
      181 agcagcgttc cggtccgtcg ggggcgaacg ttgccaccac gatcgtggcc ccgggagcaa
      241 ccgcggcgtc gagggagcgc aggtatcccg cacgctgctc cgggtcggtc atgaagtgga
      301 acaccgcacg gtcatgccac agatcccatg tgcgatccgg acgccaggtg gtgacgtcgc
      361 cgaccaccca gctcaccggc gccgagccca gtcgttcccg tgccatcgcc agcgcggccc
      421 ccgacacatc gagaacggtg aggtcggtga acccgcggtc gaccagtccg tcgaccaggg
      481 gcgacgcgcc cccgccgacg tcgatcaccc cgctgagcgg gtccacgtcc aactcggcga
      541 tgagctcgat cgacggcgtc gccaccgcct ggaaccacga caggtcccgc ggcggggtgg
      601 accggtagcg gtcgtcccag aagctcgccg ggtcctcggt cacgacgacg gcccccgagg
      661 tcggtcgagg atctggcggg cgatgggggc gaggatcagc cacgggtgcc cggccggcgc
      721 gtcgccgtcc catgccgaca tctccaccag gacccgcagg gcgtgcaggc ggcggtgcca
      781 gtcgagctgg gtcgggtcga ggtgatcatc ggcgtcggga cagagcgtcc ggtaggtcgt
      841 cgtgaagcgg cgggccatcg cccgggcgat cgggccgagc gcggtccggg ccggtgtcgg
      901 cagggcgatc ggcgggtggg ccagcacgag ctgcgtgaag gcgagtgtga acgcagggtg
      961 ggcgatcgcg gcgagcgtcc agtcgatcag gacggtgccg tcgggtccgt cgatcaggtt
     1021 gaacgggtgc aggtcgccgt gggtgaccgc cctcgtgccg ccgtcgggtc ggtggcgcag
     1081 gaggtccccg caggccccga cgagatctcc ccgtccgcga tcctcagcgg ccgcgctcag
     1141 gtgctctgcg agctcgacgg cggtgatcgg cacctcggtg ccttctgagc ggagcgctcg
     1201 ctcgaccggt gcgggatcga cggcgtgcag gcggccggcc accgaggcga gctggtcggg
     1261 gagttggcgg gtgagccggg gcaggttgcc gagcaccgcc gcggggccga ggccggcgag
     1321 gggcggtcga cccggcacgt ggtccatgac gatgacgaag ccgcccagcc cgtcggggtc
     1381 gtcgacggcc agtcggacca caggtgtcgg gatgcccgca tcggccacgc ggcgttggac
     1441 ctcggcctcg aacctcccga gcggcgagcg cgccacgatc cgggccacca gctcccggtc
     1501 gaggtccggg ggcggactct ccaggcggaa ccggagcagt ccggcgtcga agccgccgct
     1561 gatggggctc ggcgggtcgc cgaaggcgag gtcggcccga ccggtggaac ggcgcaggac
     1621 cccgagcagg agccggttgg tgtcggcgtc ggtcgtcccg gatccggcca cgaccgacag
     1681 catgtcaccc ccgtcggccg ggctgtcgcg gtgaccgacg cccggggtcc cccgataccg
     1741 gcccggcaac gaggtgtggc actgtccggg tatgcgacgt cgccccgagc ccgagatcct
     1801 cgtcccgacc cgagcgccga gccggcgcag cgtgctccgc gccggagcgc tgctgggcgg
     1861 actcggtgcc gccggagcac tcgtcggctg cggcgacgac ggatcgtcag ccggcggatc
     1921 gacgacgacc gccgcgggcg gcggggccac cacgggcgcg ctgatcgcct cgttccctca
     1981 gagcaccccc tacatcgcgg cggggtcgcc ggcccggatg ccgtacctca tcgcggaccg
     2041 tgaaggggtt ccgctgcgca gcatcgacgg tccggtgcgc ttcaccgtga gccgggacgg
     2101 cacgccggtg ggcgaaccgg tcgaggtgac gccccgctcc gacggggtgc cacgcgccta
     2161 cctgccgttg gcgttcacgt tcgcggaacc cggcatctac gacgtgagcg ccgagtacgg
     2221 gggctcgacc ctcgaggggt cggtgcaggt ggtcgatcga tcgaagatca ccactccgat
     2281 ggtcggtgac cggttgcccg cggtcgcctc accgaccacg gcgaacgcga tgggcgtcga
     2341 cccgctgtgc tcgcgggacc cggtgtgccc gtaccactcg gtcagcctcg acgccgcggt
     2401 ggcatcgggc aagcccgcga tcctggtcgt ctccactccg gcgttctgcc agaccgcggt
     2461 gtgcggaccg ttgctcgacc acgtgatgga ggccgtcggt gagcccggcg ggatcaacgt
     2521 gatccactcc gaggtgtacg cgaacccgac cgcggtcgac gacctggcca aggcgacggt
     2581 cgccccggtg cccaaggcgt tcgacatggc gttcgagccg gcgctgttcg tcgccgacgc
     2641 gtcgggcgtg gtggtcagcc gcggcgacat catcgtcgac cgcggcgagc tggccgagat
     2701 gctcgccccg atccgctgac gcgtccgtcc gctcagagcg ggtcggcgcc gacgggatcg
     2761 tcgccgacag gatcgtcctc gcggtcgtcg tctccggctg tgaccgtggc cggcaggtgc
     2821 cgggtcgaca tcagcgacag cagcgccagc acgccggtga ccagaagccc ggtcttcaac
     2881 gcccgcagct gggcctgctc gtagtcgtcg acgagcgcgt cggcggactg ctcgtcgatg
     2941 ccggcgtcgg tggccgcggc ggccaggtcg tcgctggcga cgaagtcgac gccgttgccg
     3001 gccgcggtgc tcacctgctg ggccacctcg ggggcgatgc gctcgtcggc gtcgatggtg
     3061 cggacgaaca cgttggtcag cccggccagc acgatcgccc cgatgagtgc gactccgacc
     3121 gaggagccga gctgctggcc ggtgtactgg aggccgccgg cctcgccccg gcccgacgcg
     3181 tcgaccgagg actgcacgac gttgccgagc tgcgacacga tcagacccat gccgacgccg
     3241 agcagcgcca tcgacacggc gaagccgagg tcgttcagct gcgcgtcgac cgtggcgatc
     3301 agcacgacga tcgcgagggt ggacacgacc aacccggtgc ggacgatggt gcggaccgac
     3361 acccgggccg acagacgtga cccgatcgcc gagaacagga acatcgtgat cgagatcggc
     3421 agcatccgca gtccggtgtc gagcgcgtcg aggccgatga cgagctgcag gtagagcgga
     3481 accacgaaga acacgcccat cagcaccagg ttctgggtca gcagcccgac cagcccggaa
     3541 cgcaggggga ggacctcgaa caaccgcagg tgcaccagcg ggtcccgacc ctgggcctcc
     3601 cggtggcggg accacacgac gaaggcccac agcagcagcg ctccgccgat gacgacgaac
     3661 acggcgagag agaacccgag cggctcgacc ggcgagtcct tgggggtgag gaagccccag
     3721 gtgctcgact ggaggatccc gagcacgacg gctccgatcc cggtggccga caggacggca
     3781 ccgacgacgt ccagtcgggg agcgggtccg tcgcggaggg cgtcggcgac cttcggggtg
     3841 agggccagga tgatcgcgac caggaccacc tcgccggcga acacgacccg ccacgacagc
     3901 tcggtggtcg cccaaccgcc gacgatgggc ccgaccgcga tcccggcgcc ggccacgccg
     3961 ccgatcacgg cgtaggcctg cttgcgggcg gtcccctcga agttgccggc gatcagcgcc
     4021 gccatcgccg gcagcaccag agcggcgccg atcccctcga ggaccgacca cccgagggcg
     4081 agcaccgcga ccgtcggcgc cacggcggtg gtggccgagc cgaccgcgta gatgaccagt
     4141 ccgatggtga acgcccggcg ccgaccgatg atgtcgccga tcttcccgcc ggtcagcatc
     4201 agcatcgcca tcaccaggca gtagagggtg atgaccgcct ggatggtggg gacggtggtg
     4261 tcgaagtccg caaccagctg gctgatcgac acgttcatga ccgactggtc gaggaccatc
     4321 acgaactggg cgatcgccag cgcgatgagc gccgtcttct tcatcgtcgg gtcccctgtc
     4381 ggtgagctcg gccgggaccg atgtctaccg catgggtgtc ccgtgatgta gacagttggc
     4441 tactcgcggc gcgcccgccc gtcggtctgg gatgaccgac ccgggtgccg ccccggcagc
     4501 ggacacttcc ggtccgcgaa cacaatccga catcagagag tagagacaac gatgatcctg
     4561 gccgacgtga acctgggtga gatcctctgg accacactcg tcatcttctt catggtcatg
     4621 tacttcatga tcctgttcag catcctgggc gacctgttcc gggaccacga caccagcggg
     4681 tggggcaagg ccgcgtgggt gctgttcctg gtcatcgccc cgttcctgac cgcgctgatc
     4741 tacctgatcg cccgcggcaa cgggatggcc aagcgctcca tggccgccca ggccgacgcg
     4801 cagaagcagt tcaacgagta cgtccagtcc gtcgccggca gcggatcgcc gaccgagcag
     4861 atcgctcagg ccaagcagct gctcgactcc ggtgcgatcg accaggccga gttcgaccga
     4921 ctcaaggcca aggcgctggg ctgacgcccg tcgtctccac caccgtggac ctctggctgt
     4981 cgtcctgtcg ccgtgcggcg tcggggcggc agccggttcg cgttccggcg caggtcccgg
     5041 cccggtgctg aacccgcgca cctacgaacg gggctggctg cgcttcgacc tggtcgccgg
     5101 catcgtgctg gccgcgatcc tggtcccgca gggcatggcc tacgccgagc tggccgggct
     5161 gccgccggtc accggtctct acacgacgat cgcctgcctg gtcgcctacg cgatcttcgg
     5221 gccgtcgagg gtcctggtcc tcgggccgga ctcgtcggtg tccccgctga tcctcgcgtc
     5281 gatcaccccg ttggtcgtcg tcggcgatcc gggcagcgcc atcgtgctgg cggggatgct
     5341 ggcggtgctg gtcggcctga tcgagatcgg cctcggagtc gccaagctcg gcttcgtggc
     5401 cgacctgctc tcgagcgagg tccgggtcgg ctacatgaac ggcctggccg tcacgatcat
     5461 cgtcggccag ctccccaagc tgttcgggtt ctcgaccgac gccgacgggt tcgtcgacga
     5521 gctgaaggcg ttctggaacg gactcgacca gaccaaccgg accacgctga tcaccggact
     5581 ggccgtgctg ctcgtcctgc tggtgctgcc gcggatcacc acccggatcc ccgccgtgct
     5641 cgtcgccatc gtgggcgtca ccgtcgtgtc ggccctgctc ggtctggccg acgagggcgt
     5701 gaagaccgtc ggcacgttgc cccagggctt ccccacgccg tcgctgccgt ggaccagcgc
     5761 gtccgacgcg ttggccctca tggtcggcgc cgtcggcatc acgatggtgt cgctcaccga
     5821 caccatcgcc accgcgtcga gcttcgcggc ccgtcgcggt gacgaggtcg acgccaacgc
     5881 cgagatgatc ggagtcgggg cgtccaacat cgccgccggc ttcttccagg gcttcgccgt
     5941 ctcggtcagc agctcgcgca ccgcagtcgc cgaccagtcc ggtgcgcgca cgcagctcac
     6001 cgggctggtc ggcgccgggc tggtcgcgct gctgctgctc gtgttcaacg gcctgctcgc
     6061 cgatctgccc cagaccgcgc tcgccgcggt ggtgatcgtc gcggcgctgt cgctcgccga
     6121 cctgccggcg ctcgcccgtt tcgcccggct acgtcgctcc tcgctcgtcc tcagcctggt
     6181 ggcgagcgcc ggcgtgatct tcctgggcgt gctgcagggg atcgtcgtcg ccatcgtcct
     6241 ggcgatcctg ctgttcttcc gacgcaactg gtggccccac ggaacggtgc tcgccgaggt
     6301 ccccgaactg ggcggttggc acgcggtgag cgtccacccg gaaggtcgtc aggtcgacgg
     6361 gatcgtcgtg ttccgctggg aggcgccgct gttcttcgcc aacgccggcc agttccgtga
     6421 tcaggtccgc gcgctcgtgc gcgacagctc acccgcctac gtcgtgctgc agtgcgaggc
     6481 ggtgaccgac atcgacgtca ccgcggccga ggtgctcaag gacctcgacg aggagctcaa
     6541 cgcgaagggc gtgcacctgg cgttcgtcga gctgcgtgac cgcctggccg acctgatcgt
     6601 ccgctacgac ctggacgcca cgctccaggc cgagcacttc tacgcgagcg tcgaggaggc
     6661 cgtcgccgcc ctcgcgtccg agcgtgacgg cgggcccgcc ggggcggggt cctgagatgt
     6721 ccgacgactc gacgactgcg ccggaggacg ggtcgggaca gtcccaggag ggttcccggt
     6781 ggcggcgccg caagcggccg ggtccccgtc gggccgaggg catgcccacc gggccgatcg
     6841 aggtgcgccg agccgtcatc gacgcggccg cccagctgtt ctgccgcgac ggggtcgccg
     6901 aggtcaccct gcgcgacgtc gcacagcgcg cccgggtgaa cctgggtctg atcagccgct
     6961 acgtcggcag ccgtgacgac ctggtccggg ccaccttcgc cgatctcagc gacaagctga
     7021 ccgcagaggt ccgcgccgat ccgaccgcgg cccgcgggtt cgagtccgac tcggtcatgg
     7081 tgtgctggac ccggatcctg agccacctgg tggtggtcga ccccgagacg gccgtcgagc
     7141 tcggccgggc cccgatggag gacctggtgg ccgcggcgca ggacgtctac ggccagtccg
     7201 agccggccgc ccggctgcgg gtggctcagc tgatgggctc ggccatgggc tggcggctct
     7261 tcgagccgtt cctgatggag gccgcgggtc tggaccccga ctcgatcgac gacatccgcg
     7321 ccgagctgct cggcgtgcac cggcggctgg ccgcccnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnngagg accggtcagc tgaaggaccg gtggtcggcc tcgttctggt
     7501 ccgcgtaccg gcgggcgaac tcgaccaacg acgcggtcat ggtccgaccc ttgcccaggt
     7561 aggcggcgag ggcgacgctg tcgccggtgc gggcgtgggc ccgggccagg caacggccgc
     7621 agatggctgc gtacatggcc atcgcctcgg cgggcatccg ttcgaggtcc gcggagccct
     7681 tccagtccca cagctggcgg aagtagaagt cgcgggcgac gccgtcggga ccacgcacct
     7741 ggtcccagcc caggaacacg tcgggagacg actggatgag gcgctgaccc tcgaccacac
     7801 gttggccgtg ctggccgtag cggctggcgc cgaggtgggc ctcgagcacg ctcgcgttgg
     7861 cctccttcac ctgcaggaag agcggatcgg tctcgtcgtt gccgatcagg agcgccaccc
     7921 agcagcgggt gccgacgctg ccgaccccga ccaccttccg ggccaggtcg acgaagcggt
     7981 accggtccat cagcagacgt cgatcagggg ccagcgtccg tcggtacagc ttcagcgact
     8041 cgtcgaccgc cttcatcagc gcggcgtgct ccgcgccggt caccagctcc cggaccggca
     8101 cgagcaccgg aggatcgctg cggaacctgg gggtgccgtc ggtgacctcg accagcttgt
     8161 cgaaggcccg gatgcggtcc ttgctgcggg ccttgtcgac gtttcgtcgg aagcgccgca
     8221 acacgtcatc ggaggcgcgt gccgcccacc gctgcgcgat gtcgtcgacg gtcaggcggt
     8281 cgtaccagac ctccaggttg ctcatggtcg agtactgcct catggccgac acgtacgaca
     8341 gcaccgagtc ggcgacgatc tggacccgct gggggtcgtc gaaggaacgg tcacgggcgg
     8401 ccacctcgaa gctggcgacc agtcggcgca ggtcccactc gaacggtccg ggatgcgtct
     8461 cgtcgaagtc gttgaggtcg aacaccatgg cccggtccgg cgaggcgaag acgccgaagt
     8521 tggccaggtg ggcgtcgccg cacagctgca cgtccagacc ggtgcggggc atcggtgcca
     8581 ggtcagcggc catcaccgcg gccgcacccc ggtagaacgt gaacggcgac acgcccatcc
     8641 gggagtggcg cagcgggacc aggtcctggt cgcggacctt gtcctgcgcc gagagcaccc
     8701 cgagggggtc gcggtcgggg gccggggtcc agtcgccgag cgaggcgcgg ggaaccaccg
     8761 atcgaaccgc tcgtcccgac tcggcccgct cccgcgggtc ggtcggccgc tcggatcgcc
     8821 taccggactt cttggccatg gggtccatcc tcctccgccc ggcgcacatg ccgtcgacac
     8881 cgcgaggaga gcatcatgta gacatctgat caagtcaggc gctcgtgggt cgcgccagag
     8941 ggaggatccg tgaacgagga gcgacggggc gccgacgcgc tgaccctgcc cgatccgcgc
     9001 cgtcgggcgg ccgccgcggt cgcgatcctc accgcgctcg ccgcactggc tgcgctcgca
     9061 gtcgggctgg tccggtcgtt cccccagggc ccgagccttg tcctcatggt cgtcgtcgcc
     9121 gggggactcg gcctcgacgg gttgcgtcga cggggtgcct cacggcgcat cgagttggtc
     9181 gtcgcggcgg tggtgctcgc tgccgtcgtc gtgctggccg tcgtctggcg gaccggggga
     9241 gcggcgctgg tcgcagcggt gctcctggcg ctcggtgggg tcgcggcccg gatcgcgttc
     9301 gccgcccggg tcgtccgccc tgcagcgccg cgcccgtcgc gaccggtggt gatctggaac
     9361 ccccgctccg gcggaggcaa agccgagaag gccaacctcg gcgccgaggc cgcggcccga
     9421 gggatcgagg cggtggagct ccgtcccggc gacgacctgg tgcagctggt cgaggatgcg
     9481 gtggcccggg gtgccgacgc cctggctgcg gcaggcggcg acggcaccca ggccctggtg
     9541 gccacgatcg cggcccgtga gggcctgccg ttcgcttgca tcccggccgg cacccgcaac
     9601 cacttcgccc tcgacctggg cgtggaccgc gacgacgtgg tcggagctct cgacgcgctc
     9661 gtcgacggcg gcgagcgggt ggtcgacctg gccgaggtca acggccgggt gttcgtgaac
     9721 aacgcgtcgc tgggcgtcta cgccgaggcg gtgcagcgcg acagctaccg cggcgccaag
     9781 acctcgacgt tgctgaacac cgtgcccgac gcgttcggca aggacggacc cgatcacccg
     9841 accctgcgct ggacgggtcc tgacgggcgg cccggcccac cggtcgcggc cctgctggtg
     9901 tccaacaacc cctaccggct cggcaccatc gtcggctcgg ggacacgacc cgacatggac
     9961 ggcgccaccc tcggcgtcgt gagcctgggt acgcggctcc agcggtggac cgcccctgag
    10021 ttcgtggtcg actcggaccg acccgttccg gtcggaatcg acggagaagc cctggtgatg
    10081 gatcctccgc tggtcttccg gagccggccc cgtgccctgc gcgtccgcat cgcccaggcc
    10141 catccgggcg cctcgccgtc gtcggacgcg ccggtgtcgg cgtgggccgc gctgccggcc
    10201 ctttggcgca tcgtgaccgg gcgccccggc gtcggctgat ctcggagccg cggccggcac
    10261 cgcacgggtg gccagggtgg ttcaaacagc ggctccacag ccactgcggt ccgacggtga
    10321 gtgcgaggta accacttctt attaggtcct tgacgttgtc ctgatgtccg accagagtgc
    10381 tgtcctcgct gtctgtgtgg agaggggtgg cggatggtgg gtggtggacg tccccgtgtt
    10441 tcgatcgcgg ctgtggtcgc gtcggctgtt gtggtggttg gtttgccggc catcgtgtcg
    10501 gcgtctgcgg cgccgtccgt ggaggtgaag ccccctgcgg tggtggagtc gacagcgacg
    10561 gtggcgaagg atgcgtcggc gccgcagttc gggtcggtgg ctcccgcggt cgacgtggcg
    10621 gcccctggtt cgttgccgga gctccacgct tcgagtgggg aggggtttca gctgggtcgt
    10681 tcgtcggaga aggtcgcgga tcggacagca gttcgccagc ggttccggaa tccggacggg
    10741 tcggtgacgg atcggttctc gttggagccc cggttctaca aggcgggcac cgagttgctg
    10801 ccgatcgata cgaacctgtc ggcagatggt tcccgaacgg cgaagacggt gtcggcgggg
    10861 ggcaagggtc tggccgcgac ggcgaactgg ttctccacaa agctcggcaa cctgtcgcag
    10921 ggtgtgacgg tgggtgtggg ggcggatgcg ttgacgatga cgccggtggg tgcggtgtcg
    10981 ttgccggatc cgacgtcgct ttcgaatgtg gcggtgtatc cggaggtgtg gccgggtgtg
    11041 gatctccgct accgggtcac tccgacgggg ctgaaggagg agatggtcct caagaacccg
    11101 tccgtcgctt cttcctattc gttcagtctg acgggtgcga cgacgacggg cccggtcgtt
    11161 gctgggaagg gtgacgtcgc ggcgcagttc gacgggtcgc tcggcttgtc cggctcgttg
    11221 ggtgagcggg tgatgtttgc tccgccggtc gtgacgggcg atggcgatgg acggatcttc
    11281 accgatgcga agccgacgtt gacgggttct ggttcgtcag tgtcggtggc ggttgatccg
    11341 gcgtggttgg ctgcgcagcc tgcctccgag ttcccgatcg tggtggaccc gacgatcacc
    11401 ggtcgagcgg ggacgggttg ggcgtcgttc gcggcgggtg gcgggtcggt gggtgactcc
    11461 tacgttggca acagttgtgt gaatccgccg acgtgcagcg gttacttgtg gcggtcgatg
    11521 gcgaatttca gccatggcga gctgtactcg aacttcgtgc agtccgcgac gctgcacatg
    11581 gcggccggtg cggtgggttc cccgtcgggg acgctcgggt tggtcggttg ggcgtcatcg
    11641 gccgattacg atggggcggc cacctacatc aatgggcagt cgtcggtgtt cggcatcggt
    11701 gccggcacgt cgttcgctga tgtcgacatc actgagacga tgcgtgacat ggctgaattg
    11761 gaccctggga actacggctt gttggggttc gcgttctacc agacttccgg ctactacagc
    11821 ctccaccgtc tcgggttctc gacgatcgat gtcacctacg agtccgacgg ccatgtgtcg
    11881 aacctgtcgc cggcatctgg ttcacagacg gcgcacactc gtcgtccgac gctgaccgca
    11941 acggtcggca atgcgggcgg ggcgacgacg gtgaacgtca ggttccaggt ggcgtgtccc
    12001 ggtcagtcga cgatcgattc gaactggctc gcggcgtcac cgggtacgcc gtactcgtgg
    12061 gcgttgccgt cggacatgcc gtggaacgct gagtgccgct ggtacgcggg tgggcgcaac
    12121 tcgagctacg aggactggag ccccggctac tcgatcaccg cggtctctca accggcgccg
    12181 gcgccggcgt tggaccggac gatggacggg tcgatcgtct ccactccgac gttgagcttg
    12241 tcggcgccag ggacgttgac cgatcccgag tccgacacgg tctatctcga ctactcgatc
    12301 gccaccggct ccgacggtgt gaccggccgg gtcgtccgtt ctccgctgtt ggcgttgaac
    12361 acgacgttca gcgcgccgac gtcgtcgcta ccggacggga tctactacgc gacggtgcgt
    12421 tcggtggatt cgaccggcgc cgcgtcagcg tgggtgaagc cgatcaggtt ccgggtcgtg
    12481 caacgcctcg gcgtgcaggc caagatggcc tacgactcga ccggcccgtt caacgtgaac
    12541 ctggcgtcag gcaacctcgt cgttgctgcc agtggtccgt cgttctcgac gctcggtgga
    12601 gacgccgggg tgaacttcac gtacaactcg caggccgcgg tgcccaaggg cgttcgggcc
    12661 gcgttctggg acgacaccaa cgcggacggg gtgttgcagt cgtcgggttc gccgaaggac
    12721 gcgaagcgtg ccgagcgcat cgacccggtc gcgtcgtaca actggaacga cacggcgggc
    12781 ccgatcccgg gcacggtgaa cccgaactcg ttcatcgcgg aggtgaccgg ctcgtacaag
    12841 aacacgaccg gctccgcaca gaacgtcagc ttccaagcag tcccggtcga ctcgaccggc
    12901 gccggcaaca aggtcaaggt ccaggtcaag ggcggcgccg ccgccggcgg accggagacc
    12961 tcggcgtgga acacggcgac gaccgcggtg acggtcccgg cgaacgacac ggtgtcagta
    13021 cgggtcgagt acgccaagct cgcagccgtt gcgtcatcgg ggttccgaat cgtgttctcc
    13081 gaaggctccg gctgggctga tgtccctgcg gaccgctgga tgcagtacgc gcccgtgctc
    13141 cccggcggat ggtcatcgac cggacccggg ttggggacgc tcggctactc gcggctcagc
    13201 cccggggaga accaggtcac cttgtatgac gactccggcg cagcccacgt gtggcgctcc
    13261 aacggcgaca gcttcacccc gccacccgat gaggacggtg tcctgcgccg ccagccgaat
    13321 ggattcacct acaccaacgc tgcgggaact tctgtcacgg tttcgtccgg gttcgagttc
    13381 gtcgacgacg acggctatca gtacctgttc gatgacacgg gacagatcgt gtcggtcgtc
    13441 gcacccgctg acgccgtgaa ccccggcgcg ctccgctacg cgtatgcgcg gctgtacacg
    13501 tccggtccca tgcgcatgac gcagatcaag gacccgttcg gccgcaccgt cgacctcacc
    13561 tacaaggatc cctcaacggc gaactgcccg accgacggat cctcgtcaca gccgccgtcc
    13621 tacaccctgt gcctcgttac ctacaccggg ttcacccgga actcgaccgg caccgcaccc
    13681 accaccaacc tctactacga caccgctggc aaccttgcgg ccgtcgtcaa ccccggtgac
    13741 accggcaccg gcgtggtccg accgcggacc gacttcgcct acgacgcagc cggtcgcatc
    13801 gtcggggtcc gagacagcta caccaacgac ctgatcgcat cgagcgtctt caccgcgacc
    13861 aacgttccat cctccgatcc gcactggtgg accgtcacgt acggctccgg tggaactgac
    13921 ggcatggtca cccagctcac cgccccggaa ccttcctctg gagggacccg cccgcagcag
    13981 acctacacgt ggaacctcac ctccacatat ccaggcgcga agtccgcaac acgtgtggcg
    14041 gtcgcgggat cggtgaacgc atcagggtgg acccagcacg tcgacatcga caccgcaggt
    14101 cgaccagtcc agagcatcga tcagggcggc gtcgcaatcg agacgttctg ggaccccgac
    14161 ctcgacaaac aggaccgcgt cgtcaagacg atcgaccacc actacctggc aaacccgacc
    14221 acagggctgc agaccaccac catctacgac gcagcggacc ggcccaccga cacctacggg
    14281 cccggcacac catcagagtt catcggacag acctccgcaa cagcaccaca cgccacgacg
    14341 gcctacgacg aaggcattcc cgggctcgca gcgaagtggt tcaacaacac cagcgcatcg
    14401 ggacccgccg cgctctactc gacctcgtcg ggcaccgagt cgtgggggag cggcgcccct
    14461 tccaacgcag cgttccctgg agcagccatc ggcaacgact tctccggcac cctcaccggt
    14521 caggccacgt tcgcgtcccc gaccgcgctc ggtgtccgcg cggatgccgg gcgtctgttc
    14581 gtcgacgaca tcaaggtcgg cgacacattc aacggcgcct accgggccgc tgtcaccacc
    14641 gaccagccgt ccaactactg gcgcctcgac gacaccaccg gctcgacaac cgcgactgac
    14701 atcgcctccg gtgtcaccgg cacctacatc ggagccccga ccctcggctc tgtcggtgcg
    14761 ttgaaccagg gcggttccgc cgaccccgac acctcgatca ccgtcggcgg caccggcgtc
    14821 tccggtgaga ccgccagggg cgtcaacgtc accgccccaa gcggcaccga cgcactcaac
    14881 ctctccggcg acatggcgat cgaagcgtgg gtcaacccgt cgacgacgac cggctaccag
    14941 atgatcgtcg accgctccac cagcaccgga accacagccg tctacgagct gcgcctcaac
    15001 gccgggcgcc tcgagtggtg ccaggtcaac aacggcgtca tcaccgtcgt caccgctgcg
    15061 acggctctgc ccgtcaacgc ttggtcccac gtcgcagcca cccgcatctc caccccgacc
    15121 gggctcgtca tcaagctcta cgtcaacggc gtcgacgtca ccccagccgg cggaccgttc
    15181 aacgtcgcca ccgcatcact gcccaccgac acgacacgga tcggcaaccg aaccgacggt
    15241 tgggtgttct ccggcaagct cgacgacgtc gccatctaca gccacggact ctccgcagcc
    15301 cgcgtcgcga cgcacttcgc cgcagccacc aacgccacag cagccgaact gtccacagcc
    15361 aacgcgtgga cccaaaccat ccgcgacgac ggtccgtcgc agtggtggcg tctcggcgaa
    15421 acgaccggaa ccacagcagt cgacgcgtcc agcgaaggtt ccagccccgg cgtctactcc
    15481 tcgcccacgc tcggcgcgac cggcgccatc gtcggtgacc ctgacaccgc tgtgacgttc
    15541 aacgggacga gcggcggcgt cacgctgccc gacgacgtca tctcgagccg cagtctcacc
    15601 attgagatgt ggttcaaggc taccggcgcc ggagccctgt acgggcaacg caacgccacg
    15661 agcaccaagt acgcgccgat cctctacgtc ggcaccgaca acaagctcta cggcggactc
    15721 tggggacccg gctacggcgc cgcgaacctc gtctcgaccg gctcagtcgt cggcaacggg
    15781 tggcaccacg tcgccctcac ctcgaacggt gtgtcccaga ccctctacct cgacggcgcg
    15841 caaatcggag accgctccgg cgtccccgac gtgcttggca tgccgatcgg atcgatcggc
    15901 tccgcgtgga ccaacggatg gaccgccacc aacaatgcct ggttcgtgtt caacggcacc
    15961 atcgacgacg tcgcggtata cagctacccg ctgtcgggct caagggtgaa gacccactac
    16021 gacgccctca gcctcggcga attctccggc acgaagcggg tccgagtcga ctaccaggac
    16081 cttaccggca acgccgcctt gcaagtcgtc caaggccgcc cgggctccag caccgccgta
    16141 tccgcgggag tgttgtctcc ccgatacggg ctggtcacct cgaccgtgga cccagacgga
    16201 aagaagacct cctcggagta caccaatccc gagctcggat tgaaaaccgc ggatgtcgcg
    16261 gatccggccg ggctgaacct gcggaccgtc tacacctatg agggcgccag cgccgcgaac
    16321 caacagttcc gacggcaact cacccggtcc ctcccaggga tcaccggcac cgccaccacc
    16381 ccagtcacct acggctactg ggcccaaggc tccggagcga ccaacaccgc ggacaacccg
    16441 tgtaccacgg gcaccaccga gaccatcaac caaggcggtg ccgcaaagac ccgcaccgct
    16501 gtcgaccccg acggcgccgc cagcgcgacc gcacagacct cggagacccg ctaccacggc
    16561 gacggcctga ttgccgcgac gaaacgcaac gccgagaccg gctggacgtg tttcacctac
    16621 gacggacgcg gccgaaccct cacccagacc atccccgcct tcggatcctc accccagcgg
    16681 gtcgtgacca ccaactacaa ggccgacccc gacggcgccg gtgcagcgcc ggcaacagcc
    16741 gagtggacca gcgtcaccga cgacgccgcc agcaccgtgg cgacgagccc gatcctcacc
    16801 aagaccgact ggctcggccg caccatctcc tacaccgacg ccctcggccg caccaccacc
    16861 accgcgtatg accaggcggg acgcgtcacc accacgacgg gtcccaccgg ctacggcact
    16921 gtcacgcagg catacgactc caacggacga cccactacct cgtcggtcaa ctcccaaccc
    16981 atcgccacca ccacctacaa cacctcatcg ggcgtcgcca cctcgaccac gtacccatcc
    17041 ggggcaggca acgccggcaa cggcgtcacc gtcaccaacg gcttcgacac ccgaggacgc
    17101 gccacgtcgg tcacctggac cggccccgaa tccaccgcca tcgcgtccaa caccgtgtcg
    17161 cgctccgccg cgggacgcat cgtcgacgaa gcaaccgacg gcaccatcgg tgccaccggc
    17221 gacgccaaca ccaccgggcc gaactacacc tacgacggcg ctgcgcgact caccgacgcc
    17281 tacgcccccg gacaccacta cacctacaac tacggcaccc cctccggcag cgactgccca
    17341 tccacctcac ccgtctacgc gacgctgatt gccaacaaga actccaaccg gacatcggtc
    17401 accgaccagc cactgccttc gggcactgcg acgacgacca agtcgtgcta cgacgcagcc
    17461 gaccgcctca cctccaccac ggcaggctcg atcggcacca acgtcaccta cgacacccga
    17521 ggccgaacca ccacgctcgg caccgacagt tacgcctacg accagaccga ccgccacagc
    17581 gccaccactg ccaacgcgtc cgcggcccgt aacgccctcc ttgccgtcgg cggccccgca
    17641 gccctcacct cccgcgacac ctggctgcag caacggctca tcgacgcagg atggaccgtg
    17701 acgatcggcg acgacgacac cgtcactgca tcctctgcga gcggcaagca gctgattgtc
    17761 ttgtccgagt cggtgactca ggccgggatc ggcaccaagt tcacgtccgt cgcggtgccg
    17821 ctcatctccg cagagccgtt cctctacgac gagctcggca tgaccggcac cggcaccaac
    17881 cagggcggca cgtccggcgc caccgagaca caggttgctg tcacggcagc aggtgttcaa
    17941 tccagcctcg gtgcgggcct gtccgctgga aacgccacca tcgcctcctc aggtgtcgac
    18001 ctctcctggg gcaagcccgc cgccaccgca accgtcgcag ccacgatcgc gagtgacgcc
    18061 accaaagcca cgctcttcgg ctaccgcacc ggagccgcca tggtcaccgg caccgccccg
    18121 gcaccgcgcg ttggatggac ccactacacc ggcagcccaa caccactgaa ctcaacagcg
    18181 gtcgcgctgt tcaacgccgc tattggctgg gcggccccag cctcggccac aacgagcgtc
    18241 acctacgttc gagacgtcac cgaccgcatc atcgcccgca aagtcaacgg aaacctcaac
    18301 gcctgcttca gctacaccgc aggaggcgac acccccgacc agaccctggc cccttccggt
    18361 agtagctgct cgacgaccgt caccgagacc gtgagcggac tgcccggcgg cgcgacgctc
    18421 acctcgcgcg ggaactcaac gaccgacgtg tggtccctgt ccaacatcca cggcgacacc
    18481 ctcgccatca ccaacggaaa cggcgtcaag caaggcacca ccctcaccta caaccccgac
    18541 ggccaagccc tcggcgacaa ccccgacaac agcacgggag cattcgacaa cggctggctc
    18601 ggccgccatc aacggcccct cgagcggcaa ggcaacctgc ttcagacgat cgagatggga
    18661 gcgcgtccct acagcgtcac cacaggtcgc ttcctggaga ttgatccggt cgagggggga
    18721 actgccaacg actatacgta cgtgactgat ccgctcaact cgttcgacct ggacggaatg
    18781 tgcggcgtat ttgggaatcc gttcaagaag tgcagtaagg accaaaaggg ccaacgagga
    18841 ttccttggtg gagtgttctc aaagggagcg cgcggggttc gcgtgatcat gacactgcca
    18901 gcgtcgcaac ttgggataac ctgggccaaa atgcaccgag ggaagtgctc ttgggatggc
    18961 actcactgga tgtcagtgtg cacgggcatg cgaggtgggt atgggcgtgg cggaaccacg
    19021 gtggggagcg tctacatgtc gggacgtacg ccggacgcaa ggatgatgag gcatgagagc
    19081 aaacatgcga atcaatacgc gtggtttgga ggtggagcaa tgttccccgt cgtatatggc
    19141 atccaggaat ggaccagcgg gggcggaaag cacaacacct ttgaaaggca ggctggtctc
    19201 aaggacggat gctacgtggc gaatcgccct ttctgtcgct agaccacgaa caccgcccgt
    19261 ccgtctcgcc gcaatagttg cctggtgttt gcttggggct ctttcatcag cgtcgtgtgt
    19321 tccgacctca cagccgggag tgattggagc atccggcggc cggaatagtc ttaatgcatg
    19381 gcttgcgcca tgcccttctt ctgctgtgat cggactgcag gtgtttcgct cctatccggg
    19441 ttgggaccct gagacggatc gacccctttg gagtgcccgg tttgatcggc ccctcgagac
    19501 gggaacgccg atcaacctga actcgctccc gccggatacg attgagtcag gctcaatatc
    19561 atctgctctc tcgggcggag aagtggcctt ggcagtgtct aggggaagaa ccggagcaac
    19621 cggcgttgca tacgtgacgt ttcgtccatc tgagttgtcg ccaagatcgg tctggctcga
    19681 cggcagggag atctcgcccg gagagtttcg tagatcaacc cgcgaaagct gccagtcgtg
    19741 agggggcgaa ggcgccgaga ggaggatgtc ccgttcgctg ttgaagtcgc cgcgggtggt
    19801 ggtcccggtg ttcatgcggc g
//
LOCUS       DAOQOD010000044        19050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1017_length_19050_cov_11.886339, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000044 DAOQOD010000000
VERSION     DAOQOD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_19050_cov_11.886339"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLZ72_06270"
     CDS             <1..832
                     /locus_tag="PLZ72_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNA35936.1"
                     /translation="AVIDRLAGAPSTEERRALPGMDAARADILLGGALVLEQICDGLG
                     IDELVISEYALREGVLLDGLHRLRGVSLHHLSDLRRASVEHLMELCDDDPEHSYQVAT
                     LALQLADALGDRLGLDGDDVELLHAGALLANVGLFISHSGHHKHSYYVIRNSEHLTGF
                     TDREIELIAQVARYHRKGRPADKHPEFAALSSEDQDRVRAMVCLLRVAIGLDRNHDGA
                     VERVAVTTGFTDDGAERVRLTLIGAPDADLSLEVYAAAERSNMLSDQLGATVEVVAAG
                     "
     gene            complement(837..1208)
                     /gene="mscL"
                     /locus_tag="PLZ72_06275"
     CDS             complement(837..1208)
                     /gene="mscL"
                     /locus_tag="PLZ72_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNA35937.1"
                     /translation="MLKGFKEFIAKGNAVDLAVGVVIGAAFGVVVNSITKGIIDPLIG
                     LILPGDVKDLAAKSFTVDGAEFLYGSVISAAITFVITAAAIYFLIVAPMNAWRDRGGR
                     SEDEKSNEEKMVELLERIADK"
     gene            complement(1288..1758)
                     /gene="greA"
                     /locus_tag="PLZ72_06280"
     CDS             complement(1288..1758)
                     /gene="greA"
                     /locus_tag="PLZ72_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNA35938.1"
                     /translation="MADPHELSEATRDRLQAELDELRTHGRIEMADRIERAREHGDLK
                     ENAEYHAAKEEKAKMEARIAQLFGILENAVIVEGGDSDVVRSGSVVTLRYDGDDEDEV
                     ERYLVGSIEERHDDLDVITPNSALGEALMGAAAGSSVTYATPNGEMTVVVVDIA"
     gene            1949..2947
                     /locus_tag="PLZ72_06285"
     CDS             1949..2947
                     /locus_tag="PLZ72_06285"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNA35939.1"
                     /translation="MTHRIGVIGGDGIGPEVLAEALKVLDATGVAYDRTDYDLGAHRY
                     LADGTVLPDEVLEEWRGLDALLLGAVGDPAPGVAAPAGLVERGILLRMRFDLDQYINL
                     RPFRLPPKADFEVIRENTEGTYAGEGGFLRKGTPNEIATQGSVNTRLGVERCVRFAFE
                     RAAATDRRHLTLVHKKNVLTFAGDLWQRTFDEVAEQYPQVSTGYDHIDAACIHFVERP
                     ERYDVIVTDNLFGDILTDLGGAVAGGVGYAASANLNPARTGPSMFEPVHGTAPDIAGQ
                     NKANPIAAIISLQLMLDFLGEADAAAAVAAACDNPERYTGSTTDIGDAVAAAVRSA"
     gene            3030..3947
                     /locus_tag="PLZ72_06290"
     CDS             3030..3947
                     /locus_tag="PLZ72_06290"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNA35940.1"
                     /translation="MPITPTSKIWMNGELVPWDEARIHVLTHSLHYGMGVFEGIRAYE
                     TPQGPGVFRLTEHIERLERSARIMGMTLPYSVDELVQATKDVVAASGLPSAYIRPIAY
                     YGYGEMGLNTLPCSVDVSIACWPWGAYLGDDAVTKGVRMKISTWLRHDHNAMPPASKT
                     TGNYVNSSLAKVEALNAGYDEAIMLNPSGLISECTGENIFVARHGRLVTPPVSAGALE
                     GITQSTVATIAADLGIDVVEGDLTRSDLYIAEEMFVCGTAAEVSAVNSVDDRAIPCPG
                     PKTSAIAAEYAKVVRGQVDRYKDWVELVS"
     gene            3967..5649
                     /gene="cimA"
                     /locus_tag="PLZ72_06295"
     CDS             3967..5649
                     /gene="cimA"
                     /locus_tag="PLZ72_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNA35941.1"
                     /translation="MSGLDLPDVGGDLTAVRTVDIFDTTLRDGAQFEGISLTVEDKLR
                     VAEQLDWLGVAWIEGGYPQANPKDAEFFRRAAAGELELSTATLVAFGSTRRPAGRVDE
                     DPTLAALVGAGTSTVCIVGKSWDFHVTEALGTTLDEGVAMVGESVRFLRDAGLRVFFD
                     AEHFFDGYKANPEFALRVLEAAATAEAEVLVLCDTNGGSLPHEVQRITAEVTSYFEGT
                     AVGIHTQNDSGCAVANSVAAVLGGASHVQGTVNGYGERTGNANLMTVIPDLTLKLGIA
                     TLPDGRLDRLTAVSRHVAELVNLPPHPADPYVGSSAFAHKGGLHTSALGKVGGASYEH
                     IDPAVVGNRTRVLVSDLGGRAGMAMKAAEFGVELDSRAAAELSERLKVLEAEGYVFEA
                     ADASLELLMRRATGWDHEFFSVEAYRVSSYHRQAVTPEGRPLGPVSGAAPALDLSTEA
                     TVKLWVGPDRLAAVGEGNGPVNALDAALRAALNGRYEALARIHLTDFRVRVLDSATTP
                     GLSDTGSVVRVLVDFTDGDRAWTTTGVSPNIIEASWQALTDGIVFGLLHADR"
     gene            5825..6352
                     /locus_tag="PLZ72_06300"
     CDS             5825..6352
                     /locus_tag="PLZ72_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35942.1"
                     /translation="MAAPTFVPADPVDTVRWYRSAPRRPDPWVVGRPGEVDGPQPAGE
                     GLGTPGPDAGYALTLAAAFRGTLTLRPGEHESDAIVGGAAVAMKRAGLLGRAPVAHDL
                     RVGLGVWGFLDDNPAAELVELRRDRFDEVHLTHHYPRLRRIADAVPEAVLLLGHTEIL
                     ELHGRDWRSCLDLTV"
     gene            complement(6406..7404)
                     /locus_tag="PLZ72_06305"
     CDS             complement(6406..7404)
                     /locus_tag="PLZ72_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNA35943.1"
                     /translation="MSSTGPDDPSPDDPGPGAGPGEERPWWEQDGMVVDDRPPRRLSN
                     GRIALLVAAVVAALALIGFAVVGSNDDPPSADRSTTAAPSTEPTRRPPTEARIATAKN
                     PSILVHSSPPDGWDDMEPVEVWDAPVPPSSQASTAARPPLPRDDYPIKGRYTDPSGWS
                     FSNPSGWGDPFVMLVTEQRGDWLKVEIPVRPNGTPGYVAAVDVELSTTDYRLDLRLGT
                     RTLTLYKGDDVVMTTPVVIGKPETRTPTGRFYITDLVHQEDPAGAYGPLALPTNAYSE
                     QIDEFDNGVPVIALHGTNRPELMGQDVSNGCIRLPNDKIQMLADTIPMGTPIDVWA"
     gene            7489..8907
                     /locus_tag="PLZ72_06310"
     CDS             7489..8907
                     /locus_tag="PLZ72_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNA35944.1"
                     /translation="MTNPEPAPSGPVRVRFAPAPTGYLHLGSARAALFNWLTARHLGG
                     TFLLRIEDTDLERSQQALIDVVFEALEWLGLDWDEAPVLQSTRSEHHQAAVDRLLADG
                     SAYWSDPVPEADRGRTGGRPYDPADRDRDLGPGPGRAVRFRTPDDGTTAWTDLIRGDI
                     SFDNATIEDFVIRRADGSPTFFVANAVDDHDMAVTHVIRGEDLINVTPKQLLLRAALG
                     DDRPLELGHMPLIVNEQRKKLSKRRDDVSLLDYRDRGFLPAAMVNYLALLGWGLPDGS
                     EIRLDPLRELPPLFDIADVNDSSASFDAKKLRFVNAEHLRALPVTEFARLVEPFVAAE
                     PWADRFHAGTFGRIAADIQVRAETLAEVPAQVDFLFRSDPDIDEASWTKAMVPEAAGW
                     IDAVIAAAEEWPWEATELHQLTFALADRLDANRRKFQAPIRVALTGRTVGPPLFESME
                     VLGRDEVVRRLRAARERLGATG"
     gene            8894..9538
                     /locus_tag="PLZ72_06315"
     CDS             8894..9538
                     /locus_tag="PLZ72_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNA35945.1"
                     /translation="MPPAEERPSLRARARAHRRRWIGRLVLAAVLALVGYLVLTFVQV
                     LSASRDDDRVRSDAIVVLGAAQYDGTPSPVLRQRLDHALDLYRDGVAGRIVLTGGKQA
                     GDRFTEAYAGYRYLTGRGVPGDDLLVVTDGSSTWDSLRAAERVLRREGLDRVTLVSDS
                     YHSRRLLGVADEVGLDAGVSPSGAAPTVPELLRETGLVALGQVIGYGRMLRLSS"
     gene            complement(9558..11696)
                     /locus_tag="PLZ72_06320"
     CDS             complement(9558..11696)
                     /locus_tag="PLZ72_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35946.1"
                     /translation="MPHATASGPIVSVDDTGTFRIGPDGADWLGPGRPTAAVADGRVT
                     ATVEVDADRSWVVIALEALVDLDGLATGAFDRPSVGVEFTPTDRRVGDHPVEQEAQPH
                     RALAFQHCEFALPATAGADLDGFFLLPHRPPTGWPLLVSDHSGRTVMLAPLEDFHEQT
                     IGLNGGTVRVGWHGDLERVPRGFRTELGVFTGPSARSCLETWGSLLQERAGTVRPGRW
                     ADELARRPSYWTDNGAAYWYRTEPGLDAASSIVAAVDDLRGRGVPLGAVQLDSWFYPH
                     VESRPFDTDEWTVPPSAMVAWEPRDDVLPDGIPALRRRLGDPPLVAHIRHLSAQAPIA
                     SEVPVHLDGPYAAPATAEAYERWLDQCVAWGVETFEHDWLVEVFFGVRELRSEPGRAR
                     DWQEGIDRAASERGITLQWCMGTPADLARSTTLTQVTSVRTCGDHGYIATAGQLWAWF
                     AVTNALARVLGLMPFKDVFRTDAEVAGANGEPEALLSALSTGPVGLGDRVGRFDDAIV
                     PRICRADGLLVKPDVPVAATSASMLANPAFNPTPLVAECHSDHPAGRWCYVVTMHCCP
                     TDDPVDGVLDLADLDDGRPDGDVVLWDWRARTAERVGPDHRRPIHLGREDWRYLVLAP
                     LLDSGMAVIGDVEKFVTAGDSRVRVRAIDGGVSLVVLGAGEDVTVTGWSELPPRSSTA
                     PVVHDPVSGVWELTVAVPARGWTTVEVLAG"
     gene            complement(11755..12528)
                     /locus_tag="PLZ72_06325"
     CDS             complement(11755..12528)
                     /locus_tag="PLZ72_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35947.1"
                     /translation="MTTTPNTTASPTTPTRRRRALAVGIVGLSALVPVALPAAAGAED
                     SRSAPPTTSEACKPFTHARERIAGEITRRQATIVELVASLQAASDPYGVNGGQIVALQ
                     SSANGLATLGQQIATACYPDVASLRADAQKVLFEHRIYALRVPQSRVIEAADRLGTAR
                     NELQSVADTLATKVGDDAEARAELDAMNQALADADSVIGRPPTLTGAPADVVALEPAQ
                     DLDPTRAALTAARDAVKAAHQDLASARDHAKAAVAALRS"
     gene            complement(12525..12920)
                     /locus_tag="PLZ72_06330"
     CDS             complement(12525..12920)
                     /locus_tag="PLZ72_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35948.1"
                     /translation="MDHDDRPGRHRPRRSAAARIVRLSAGTLGAVAVVALGAGCGNAR
                     ADDGSTPASVSRNAAETTTVDEPPAPSTAPTSTAAPATVDGADTASADSLADLDARID
                     GAGRSLDAADTATRQATDALNTDNEGTIP"
     gene            13022..14224
                     /locus_tag="PLZ72_06335"
     CDS             13022..14224
                     /locus_tag="PLZ72_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA35949.1"
                     /translation="MTVTDTDQLRYRFRGGETWREPWDDYRWLRDHDPVHRVDHPTQG
                     TFWVLSRFDDVFDAVRDTATFSSAQGLTPDSDVMGFFEGQAAPLVMLDPPEHTAMRRL
                     VSRPMTPSRVDALAPTIAAFVDERLGRLAELSGGGSPVDVVEVLLKPLPSMLVAHFLG
                     IPEQDRGRFDGWTDAIVAANSREDLGSAMHAFAELFAYSESLIERRRTDPGDDVVSDL
                     VTADVDPRWITGFVFTMIAGGNDTTTGLLGGSLELLTARRDQRLLLIEDLSLVRPAVD
                     ELLRLTSPVQNLARTATVEVERAGVRIPAGAKVMLLYGSANRDERQYGPTADELDVTR
                     NPERILSFGYGAHHCLGAAVARLAATITLERILERFPDFVVDAASGRFAPGPYVRRYE
                     TLPFLGVP"
     gene            complement(14265..14630)
                     /locus_tag="PLZ72_06340"
     CDS             complement(14265..14630)
                     /locus_tag="PLZ72_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNA35950.1"
                     /translation="MASITMRTFDDPDETRRPDKTQMDIVDLGTAKVARLTAQPGWTW
                     SECIKPIAGTDSCQTRHVGVVQSGSLTVTHDDGTEMVLTAGNVYVIEPGHDARNDGNQ
                     PFVALEFESHTAEMYAKDS"
     gene            14801..15295
                     /locus_tag="PLZ72_06345"
     CDS             14801..15295
                     /locus_tag="PLZ72_06345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA35951.1"
                     /translation="MRYRDCPTVEVSQRVAASPDRAWRAVCDIGLPARCGGELQAARW
                     LDGATEPAVGARFEGVNRNEHLGEWTTTSTVVEVEPGRRWVWEVADVASGPWSRWGFE
                     VDPVRDGVVMRQWARIGPGRSGLSLAIDAMPDREARIVEVRMAELADAMRGNLDRVRT
                     ELEG"
     gene            15362..17035
                     /locus_tag="PLZ72_06350"
     CDS             15362..17035
                     /locus_tag="PLZ72_06350"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNA35952.1"
                     /translation="MSTQPEPIDFVREKVRDDRAAGRFGGRVQTRFPPEPNGFLHVGH
                     AKSVYLNFGIAEEFDGICHLRFDDTNPVAEDVSYVEAIVEDLAWLGVTPSGPPRFTSD
                     SFDQLYLWAEDLIRAGLAYVDDQDQATISAQRGGFGQPGVESPHRNRSVEDNLERFRG
                     MRDGRYSDGECVLRARIDMQADNMWLRDPVLYRIRRAHHHRTGDRWIIYPTYDWAHGQ
                     VDAIEGTTHSICTLEFAEHRPLYDWCLDHVELPSDRPEQTEFARLNLTHTVLSKRVLR
                     TLVEDGLVDGWDDARMPTVRGLRRRGYPAEALRAFCDHISVAKTNSTVEIELLESFVR
                     THHNRHALRRMAVLDPLRLTITNWPEGHVEWMDVINNPEDDAEGTRAVPFTGELWIER
                     DDFMLDPPPKYFRLTPGREVRLRNAFFVTATDVVLDDDGNVVEVLATYDPESRGGAAP
                     DGRKVKSTLHWVSRPHAADATVHLYERLFRSAHPGADGVDPLEDLDRGSRTTRSAKVE
                     PALAEADPGQVVQFERLGYFAADLDDPSVFHRTVGLRDEWANIQKQRTT"
     gene            17122..18528
                     /locus_tag="PLZ72_06355"
     CDS             17122..18528
                     /locus_tag="PLZ72_06355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA35953.1"
                     /translation="MIDRLGALDAEFLHVEDGVGHMHIAGACTFDGPPPTFDDIEQMV
                     RSKMHLIPRYRQRVRSVPFELGRPIWVDDPHFDLRYHLRHTALPAPGDDAALCRLMGR
                     VMSQPLDRDRPLWETWLVEGLEGGRWALVFKVHHCMVDGISGVGLLTVLLDIGPESDP
                     VEEQPWSPTAEPGVARKVLGTWSGLAADLSTQAHRVGSALAHPVDAGRDVVGLVAGTA
                     RLGRALTATPQSPIDGSIGPHRTWAFSSASFDDVRAVRAECGGTVNDIVLTAVTAGYA
                     ALLRHRSEDLDRAVVRTLVPVSTRPTSGAGVPDNRVSLMLLELPVGVADHLDRLRVIQ
                     GRMTELKASHMVEVGELATTLGDLAPPMVIGPASRWLVGLLRRVPQRSVNTVTTNVPG
                     PQFPLYCLGREMLSYLPYVPITHGLRVGTAILSYNGRLFFGVTGDAATTPDVDVLADA
                     TAAAVGEMRDQVGATATR"
BASE COUNT         2640 a         6730 c         6818 g         2862 t
ORIGIN      
        1 ggcggtgatc gatcggctcg ccggcgcgcc gagcacggag gagcgtaggg ccctgcccgg
       61 aatggacgcg gcccgagctg acatcctgct cggcggcgcc ctggtgctgg agcagatctg
      121 cgacgggctc ggcatcgacg agctggtgat ctcggagtac gccctgcgcg aaggcgtgct
      181 cctcgacggg ctgcaccgcc tccgcggcgt gtcgctgcac cacctgtcgg atctgcgccg
      241 ggcctcggtc gaacacctga tggagctctg cgacgacgac cccgagcact cgtaccaggt
      301 cgccaccctc gccctgcagc tcgcggacgc gctgggggac cgtctcggcc tggacggcga
      361 cgacgtcgag ctgctgcacg ccggggccct gctcgccaac gtcgggctgt tcatcagcca
      421 ctcgggtcac cacaagcaca gctactacgt gatccggaac tccgagcacc tcaccggatt
      481 caccgaccgg gagatcgagc tcatcgccca ggtggcccgc taccaccgca agggtcgccc
      541 ggccgacaag catcccgagt tcgccgcgtt gtcgagcgag gaccaggacc gggtgcgggc
      601 gatggtgtgc ctgctgaggg tggccatcgg cctggaccgc aaccacgacg gtgccgtcga
      661 acgggtcgcc gtcaccacag ggttcaccga cgacggagcc gagcgggtgc ggctcaccct
      721 catcggcgcg cccgacgccg acctgtcgct cgaggtctac gcggccgccg agcgttcgaa
      781 catgctctcg gatcagctcg gtgcgaccgt cgaggtcgtc gccgcgggct gagaggtcac
      841 ttgtcggcga tgcgctccag cagctcgacc atcttctcct cgttggactt ctcgtcctcg
      901 gatcggccgc cccggtcacg ccaggcgttc atcggggcca cgatcaggaa gtagatcgcc
      961 gccgcggtga tcacgaacgt gatcgcggcc gagatcacgc tgccgtagag gaactcggct
     1021 ccgtcgacgg tgaaggactt ggcggccagg tccttgacgt cgccgggcag gatgaggccg
     1081 atcagcgggt cgatgatccc cttggtgatc gagttgacca cgaccccgaa cgcggcgccg
     1141 atgacgacgc cgaccgccag gtcgacggcg ttgcccttgg cgatgaactc cttgaacccc
     1201 ttcaacatgc tgctcactcc tgtctggacg accgccccat gatgggcgcc ggtcgatcgg
     1261 caggggtgga ccgcggggtg gatccgatca ggcgatgtcg accacgacga cggtcatctc
     1321 gccgttgggc gtggcatagg tgacggagct gccggccgcc gcgcccatca gcgcctcgcc
     1381 cagagcggag ttcggggtga tcacgtcgag gtcgtcgtgg cgctcctcga tcgagccgac
     1441 caggtaccgc tcgacctcgt cctcgtcgtc gccgtcgtac cgaagggtca ccacgctgcc
     1501 ggagcggacc acgtcggagt cgccgccctc gacgatgacc gcgttctcca ggatcccgaa
     1561 cagctgggcg atccgggcct ccatcttggc cttctcctcc ttggccgcgt ggtactcggc
     1621 gttctccttg aggtcgccgt gctcgcgggc ccgctcgatg cggtccgcca tctcgatgcg
     1681 cccgtgggtg cggagctcgt cgagctcggc ctggaggcgg tcacgggtgg cttcggacag
     1741 ctcgtgggga tcggccatgg cgggccagcc tacgagcgct gcggagcgcg cgggtacact
     1801 ggcgcccgtg acccacacgt gcgcgttccc gaccgtcgtc cggtccgaca tcattcgcta
     1861 gcgcacgccc caccggcgtg cgcgttctga ccgtccacct cgtgggcggt tttcttttgt
     1921 ccgagaccga gagacaggca gagcagacat gacccacagg atcggcgtga tcggcggcga
     1981 cggaatcggc cccgaggtgc tggccgaggc gctgaaggtc ctcgacgcca ccggcgtggc
     2041 ctacgaccgc accgactacg acctgggcgc gcaccgctac ctggccgacg gcaccgtgct
     2101 tcccgacgag gtgctcgagg agtggcgcgg gctcgacgcc ctgctgctcg gcgcggtcgg
     2161 cgatccagct cccggcgtgg ccgccccggc cggactggtc gagcggggga tcctgctgcg
     2221 catgcgcttc gacctggacc agtacatcaa cctgcgtccg ttccgcctcc cgcccaaggc
     2281 cgacttcgag gtgatccgcg agaacaccga gggcacctac gccggcgagg gcgggttcct
     2341 gcgcaagggc acccccaacg agatcgccac ccagggctcg gtcaacaccc gcctcggcgt
     2401 cgagcgctgc gtccgcttcg cgttcgaacg cgccgcggcc accgatcgac gccacctgac
     2461 gctggtgcac aagaagaacg tgctgacctt cgccggcgac ctgtggcagc gcaccttcga
     2521 cgaggtcgcc gagcagtacc cgcaggtgag caccggctac gaccacatcg acgccgcctg
     2581 catccacttc gtggagcgtc ccgagcgcta cgacgtgatc gtgaccgaca acctcttcgg
     2641 cgacatcctc accgacctgg gcggcgcggt cgccggcggg gtcggctacg cggcgtcggc
     2701 caacctgaac ccggcccgca ccggcccgtc gatgttcgag ccggtccacg gcaccgcccc
     2761 cgacatcgcc gggcagaaca aggccaaccc gatcgcggcg atcatctcgc tgcagctcat
     2821 gttggacttc ctgggcgagg ccgacgccgc cgccgcggtc gcggcggcct gtgacaaccc
     2881 ggagcgctac accggctcca ccaccgacat cggcgacgcc gtcgccgccg cggtcaggag
     2941 cgcctgacca ccgccaacga gaacaggggt cggaatcgac gcatcctgtc gattccgagc
     3001 cccagaaccg cacgcacgaa ggaaccacca tgcccatcac ccccaccagc aagatctgga
     3061 tgaacggcga gctcgtcccc tgggacgagg cccgcatcca cgtgctgacc cacagcctcc
     3121 actacggcat gggcgtgttc gagggcatcc gggcctacga gaccccgcag ggtccgggtg
     3181 tgttccgact caccgagcac atcgaacgcc tggagcgctc ggcgcgcatc atgggcatga
     3241 cgctgccgta ctcggtcgac gagctggtgc aggcgaccaa ggacgtggtg gccgccagcg
     3301 ggctgccgtc ggcctacatc cgccccatcg cctactacgg ctacggcgag atgggcctca
     3361 acaccctgcc gtgctcggtc gacgtgtcga tcgcgtgctg gccgtggggc gcgtacctgg
     3421 gcgacgacgc cgtcaccaag ggcgtgcgca tgaagatcag cacctggctg cgccacgacc
     3481 acaacgccat gcccccggcg tcgaagacga ccggcaacta cgtcaactcc agcctggcca
     3541 aggtcgaggc gctcaacgcc ggctacgacg aggcgatcat gttgaacccg tcggggctga
     3601 tctcggagtg caccggcgag aacatcttcg tcgcccgcca cggccgcctg gtcaccccgc
     3661 cggtctcggc cggggcgctc gagggcatca cccagtcgac cgtcgccacc atcgcggccg
     3721 atctcggcat cgacgtggtc gagggcgacc tgacccgctc cgacctctac atcgccgagg
     3781 agatgttcgt ctgcggcacc gcggcggagg tctcggcggt caactcggtc gacgaccggg
     3841 cgatcccgtg cccgggcccc aagacctcgg cgatcgcggc cgagtacgcc aaggtcgtcc
     3901 gcggccaggt cgaccgctac aaggactggg tggagctcgt ctcatgagtg cgtcgcgagc
     3961 gttccgatga gcggcctgga cctgcccgac gtcggcggcg acctcaccgc ggtccggacc
     4021 gtcgacatct tcgacacgac gctgcgcgac ggcgcccagt tcgagggcat ctcgctgacc
     4081 gtcgaggaca agctgcgggt cgccgagcag ctcgactggc tcggagtggc ctggatcgag
     4141 ggcggctacc cgcaggccaa ccccaaggac gccgagttct tccgtcgcgc cgcggcgggc
     4201 gagctggagc tgtcgaccgc cacgttggtg gcgttcggct cgacccgccg gcccgccggc
     4261 cgtgtcgacg aggacccgac gctggccgcg ctggtcggcg ccggcacgtc gacggtctgc
     4321 atcgttggca agtcgtggga cttccacgtc accgaggcgc tcggcaccac cctggacgag
     4381 ggcgtggcca tggtcggcga gtcggtccgg ttcctgcgcg acgccggcct gcgggtgttc
     4441 ttcgacgccg agcacttctt cgacggctac aaggccaacc ccgagttcgc cctgcgggtg
     4501 ctcgaggccg ccgccaccgc ggaggccgag gtgctggtgc tctgcgacac caacggtggg
     4561 tcgctgccgc acgaggtgca gcgcatcacc gccgaggtca cctcgtactt cgaagggacc
     4621 gccgtcggta tccacaccca gaacgactcg ggctgcgcgg tggccaactc ggtcgccgcg
     4681 gttctcggcg gggccagcca cgtgcagggg accgtcaacg gctacggcga gcggaccggc
     4741 aacgccaacc tgatgacggt catccccgac ctgaccctca agctcggcat cgccacgctg
     4801 cccgacggcc gcctggaccg cctcactgcg gtgtcacgcc acgtcgccga gctggtcaac
     4861 ctgccgccgc accccgccga cccctacgtc gggtcctcgg cgttcgccca caagggcgga
     4921 ctgcacacct cggcgctggg caaggtcggc ggggccagct acgagcacat cgaccccgcc
     4981 gtggtcggca accggacccg ggtgctggtg tcggacctgg gcggccgggc cgggatggcc
     5041 atgaaggccg ccgagttcgg tgtcgagctc gattcccgcg ccgccgcgga gctgtcggaa
     5101 cggctcaagg tcctcgaggc cgaggggtac gtcttcgaag cggccgacgc gtcgctcgag
     5161 ctgctcatgc gccgggcgac cggttgggac cacgagttct tctccgtcga ggcgtaccgg
     5221 gtgtcgagct accaccgtca ggccgtgacc ccggagggcc ggccgctcgg gccggtgtcg
     5281 ggcgcggcgc cggcgctcga cctgtcgacc gaggcgacgg tgaagctgtg ggtcggtccg
     5341 gaccggctcg ccgcggtggg ggagggcaac ggcccggtca acgccctcga cgccgcgctg
     5401 cgcgccgcgc tgaacggtcg ctacgaggcg ctggcccgca tccacctgac cgacttcagg
     5461 gtgcgcgtgc tcgacagcgc caccacgccg ggcctgtccg acacgggctc ggtggtccgg
     5521 gtgctggtcg acttcaccga cggcgaccgc gcctggacca cgaccggggt gtcgccgaac
     5581 atcatcgagg cgtcctggca ggcgctgacc gacggcatcg tcttcgggct cctgcacgcc
     5641 gaccgctgag cccccccggc gatcttgtag ctggcggtcg cttgggtggc gtgtgggcta
     5701 caagttcggt gtgcgccggc gatgttgtag ctggtggttg ctggggtggc gtgtgggcta
     5761 caagttcggt ggcgggcgcc gatgattccc gggccggcgg cgggcggcgc taccgttcgc
     5821 agcgatggcc gcgcccacct tcgtccccgc cgatcccgtc gacaccgtgc gctggtaccg
     5881 cagcgcgccg cgtcgacctg acccgtgggt cgtcggccgc ccgggcgagg tcgacggccc
     5941 ccagcccgcc ggtgagggcc tgggcacacc cggtccggac gccggctacg cgctgaccct
     6001 ggctgcggcg ttccggggga ccctgacgtt gcgccccggc gagcacgagt cggacgcgat
     6061 cgtcgggggt gccgcggtgg ccatgaagcg ggcgggtctg ctcggtcggg cgccggtcgc
     6121 ccacgacctg cgcgtcggcc tgggcgtgtg gggcttcctg gacgacaacc cggccgccga
     6181 gctggtcgag ctccggcgcg accgcttcga cgaggtccac ctcacgcacc actacccgcg
     6241 actccgccgg atcgccgacg cggtgcccga ggcggtcctg ctcctgggcc acaccgagat
     6301 cctcgagctc cacggccgcg actggcgcag ctgcctcgac ctgacggtct gacggtctgc
     6361 ccgactgcct gacggtcgat ccaacggccg ggcgatcggg tcgcctcagg cccagacgtc
     6421 gatcggcgtg cccatcggga tcgtgtcggc cagcatctgg atcttgtcgt tggggagtcg
     6481 gatgcagccg ttcgagacgt cctggcccat cagctcgggc cggttcgtcc cgtgcagggc
     6541 gatgacgggc acgccgttgt cgaactcgtc gatctgctcg ctgtaggcgt tggtcggcaa
     6601 cgcgagcggt ccgtaggcgc cggccgggtc ctcctggtgg acgagatcgg tgatgtagaa
     6661 gcggccggtc ggcgtccggg tctcgggctt gccgatcacc accggcgtgg tcatcaccac
     6721 gtcgtcgccc ttgtacaggg tcaacgtgcg ggtgccgagc cggaggtcga gccggtagtc
     6781 ggtcgtcgac agctccacgt cgaccgccgc gacgtagccg ggggtgccgt tcgggcggac
     6841 ggggatctcg accttcagcc agtcgccgcg ctgctcggtg accagcatca cgaacgggtc
     6901 gccccagccc gacgggttgg agaagctcca cccggacggg tcggtgtagc ggcccttgat
     6961 cgggtagtcg tcacggggca gcggcggccg ggcggccgtg ctcgcctggc tgctgggcgg
     7021 aacgggggcg tcccacacct cgacgggttc catgtcgtcc cacccgtccg gtgggctgga
     7081 gtggaccagg atcgacgggt tcttcgccgt ggcgatccgg gcctcggtcg gggggcgtcg
     7141 cgtgggctcg gtcgacggcg ccgcggtcgt ggagcggtcg gcggacggcg gatcgtcgtt
     7201 cgagcccacc acggcgaatc cgatcagggc cagcgcggcg accacggcgg cgacgagcag
     7261 cgcgatccgt ccgttggaga gccgtcgggg agggcgatcg tcgacgacca tcccgtcttg
     7321 ctcccaccac ggccgttcct cgcccggtcc ggcgccggga ccggggtcgt cgggactggg
     7381 gtcgtcggga ccggtgctgc tcatcggtgc tccatgtcga ctcgacatcc taggagggca
     7441 ggtcgccgcg gcccgggcgc ccgtcggcca accggtagcg tgacgcccgt gacgaacccc
     7501 gagcccgccc cctccgggcc ggtacgtgtc cgcttcgccc ccgcccccac gggctacctg
     7561 cacttgggca gcgcacgagc cgcgctcttc aactggttga ccgcccgcca cctcggcggc
     7621 acgttcctgc tgcgcatcga ggacaccgac ctcgaacgct ctcagcaggc cctcatcgac
     7681 gtggtcttcg aagcgctcga gtggttgggc ctcgactggg acgaggcgcc ggtgctgcag
     7741 tcgacccgtt cggagcacca ccaggccgcg gtcgaccggc tcctcgccga cggctcggcc
     7801 tactggtccg atccggtgcc ggaggccgat cgtggacgca ccggcggacg cccctacgac
     7861 ccggccgatc gtgaccgcga ccttggtccc ggcccgggcc gtgccgtgcg gttccggacc
     7921 cctgatgacg gcacgacggc gtggaccgac ctgatccgcg gcgacatcag cttcgacaac
     7981 gccaccatcg aggacttcgt gatccggcgg gccgacgggt ccccgacctt cttcgtcgcc
     8041 aacgccgtcg acgaccacga catggcggtg acccacgtga tccgcggcga ggacctgatc
     8101 aacgtcaccc ccaagcaact gctgctccgg gctgcgctgg gcgacgaccg accgctcgag
     8161 ctcgggcaca tgcccctcat cgtcaacgag cagcgcaaga agctgtccaa gcgccgtgac
     8221 gacgtgtcgc tcctcgacta ccgcgaccgt gggttcctgc ccgcggcgat ggtcaactac
     8281 ctggccctcc tcggctgggg gctgcccgac ggctccgaga tccgcctcga cccgctgcgg
     8341 gagctcccgc cgctgttcga catcgccgac gtcaacgact cgtcggcctc gttcgacgcc
     8401 aagaagctgc gcttcgtcaa cgccgagcac ctgcgggccc tgccggtcac cgagttcgcc
     8461 cgactcgtcg aaccgttcgt cgccgccgaa ccgtgggccg accgcttcca cgcgggcacc
     8521 ttcggtcgga tcgccgcgga catccaggtt cgcgccgaga ccctcgccga ggtccccgcc
     8581 caggtcgact tcctgttccg gtccgacccc gacatcgacg aggcgtcctg gaccaaggcg
     8641 atggtgcccg aggcggccgg ctggatcgac gcggtcatcg ccgccgcgga ggagtggccg
     8701 tgggaggcca ccgagctgca tcagctcacg ttcgccctgg ccgaccggct cgacgccaac
     8761 cggcggaagt tccaggcccc gatccgcgtc gcgctcacag gacggaccgt cggcccgccg
     8821 ctgttcgagt cgatggaggt gctggggcgc gacgaggtcg tgcgccggct gagagcggcg
     8881 cgggagcgcc tcggtgccac cggctgagga gcgtccgtcg ctgcgggcgc gggcccgtgc
     8941 ccatcggcgc cgctggatcg gtcgactcgt gctggccgcg gtgctcgccc tggtcggcta
     9001 cctggtgctg accttcgtcc aggttctgtc ggcgtcgcgg gacgacgaca gggtccgcag
     9061 cgacgcgatc gtcgtgctcg gcgccgcgca gtacgacggg acgccctcgc cggttctgcg
     9121 ccagcgactc gaccacgccc tcgacctgta ccgcgacggc gtggccggac ggatcgtgct
     9181 caccggcggc aagcaggccg gcgaccgctt caccgaggcc tacgccggct accgctacct
     9241 gaccggccgg ggcgttcccg gcgacgacct gctggtcgtg accgacggct cctcgacgtg
     9301 ggactccctc cgcgccgctg agcgcgtcct gcgtcgcgag ggcctcgatc gggtgacgtt
     9361 ggtgtccgac agctaccact cccgtcggct gctgggcgtc gccgatgagg tcggcctcga
     9421 cgccggggtc tcgccgagcg gtgcggcacc cacggtgccc gagctgctgc gcgagaccgg
     9481 gttggtcgcg ctcggtcagg tgatcggata cggccggatg ctgcgcctgt cgagctgacc
     9541 acgccgccgc gggtagctca gccggccagc acctcgaccg tcgtccagcc ccgtgccggc
     9601 accgcgacgg tgagctccca gacccccgag acggggtcgt ggacgacggg agcagtgctc
     9661 gagcgtggcg ggagctcgga ccagcccgtg acggtcacgt cctcgccggc tccgagcacg
     9721 accaggctga cgcccccgtc gatggcccgg acccggaccc gggagtcgcc ggcggtcacg
     9781 aacttctcga cgtcgccgat gacggccatc ccggagtcga gcagcggagc cagcaccagg
     9841 taccgccagt cctcccgccc gaggtggatc gggcgacggt gatcgggccc cacccgctcg
     9901 gcggtgcggg cccgccagtc ccagaggacc acgtcgccgt cgggccgtcc gtcgtcgagg
     9961 tcggccaggt ccaggacgcc gtcgaccggg tcgtcggtcg gacagcagtg catggtcacc
    10021 acgtagcacc accgtccggc ggggtggtcg ctgtggcact cggcgaccag cggggtcggg
    10081 ttgaacgccg ggttggccag catcgacgcg ctggtggcgg cgaccgggac gtcgggcttc
    10141 accagcaggc cgtcggcgcg acagatccgc ggcacgatcg cgtcgtcgaa ccggccgacg
    10201 cggtcgccga ggccgaccgg accggtcgac agcgccgaga gcagcgcctc gggttcgccg
    10261 ttggcgccgg ccacctcggc gtcggtgcgg aacacgtcct tgaacggcat caggccgagg
    10321 acccgggcca gcgcgttggt gacggcgaac cacgcccaca gctgtccggc ggtggcgatg
    10381 tagccgtggt cgccgcaggt gcgcaccgac gtgacctggg tgagggtggt gctgcgggcg
    10441 agatcggccg gggtgcccat gcaccactgg agcgtgatcc cgcgttcgct ggcggcgcgg
    10501 tcgatgccct cctgccagtc ccgggcgcgt cccggttcgc tgcgcagttc gcggaccccg
    10561 aagaagacct cgaccagcca gtcgtgctcg aaggtctcga ccccccaggc gacgcactgg
    10621 tcgagccacc gctcgtaggc ctcggcggtg gccggcgccg cgtacgggcc gtcgaggtgc
    10681 accggaacct ccgacgcgat gggagcctgg gccgacaggt gacggatgtg ggcgaccagc
    10741 ggcgggtccc ccaaccgccg gcgcagtgcc gggatcccgt cgggcaggac gtcgtcgcgg
    10801 ggttcccagg cgaccatggc gctgggcggg acggtccact cgtcggtgtc gaacggccgc
    10861 gactcgacgt gggggtagaa ccacgagtcg agctgcaccg ctccgagcgg aacgccccgg
    10921 ccccgcaggt cgtcgaccgc ggcgactatc gacgacgcgg cgtccaaccc cggttcggtc
    10981 cggtaccagt aggcggcgcc gttgtcggtc cagtacgacg gtcgccgggc cagctcgtcg
    11041 gcccagcggc ctggccggac ggtcccggcc cgctcctgca gcagcgatcc ccaggtctcc
    11101 aggcaggatc tggcactcgg gccggtgaac acgccgagct cggtgcggaa ccctctcggc
    11161 acccgctcca ggtcgccgtg ccaaccgacg cggaccgtcc cgccgttgag cccgatcgtc
    11221 tgctcgtgga agtcctcgag cggggcgagc atcacggtgc gtcctgagtg gtccgacacc
    11281 agcagcggcc aaccggtcgg cggtcggtgc ggcagcagga agaacccgtc gaggtcggcc
    11341 ccggcggtgg cggggagcgc gaactcgcag tgctggaagg ccagagcgcg gtgcggctgg
    11401 gcttcctgct cgacgggatg atctccgacg cgccggtcgg tcggggtgaa ctccaccccg
    11461 accgacgggc ggtcgaacgc cccggtggcc aggccgtcga gatcgacgag cgcctccagg
    11521 gcgatcacca cccaggaccg atcggcgtcg acctcgacgg tggcggtgac ccgaccgtcg
    11581 gcaacagccg cggttggccg gccgggcccc aaccagtcgg cgccgtcggg gccgatgcgg
    11641 aaggtcccgg tgtcgtcgac cgagacgatc ggtccggagg cggtggcgtg tggcaccggc
    11701 gcagtctggc ccgaccggca gccaccgtgc atcggaggtg tcgccgagcc gggatcagga
    11761 ccgcagggcg gcgacggcgg ccttggcgtg gtcccgggcc gaggccaggt cctgatgggc
    11821 cgccttcacc gcgtcgcggg ccgcggtgag cgcggcccgt gtcggatcga ggtcctgggc
    11881 gggctcgagg gcgacgacgt cggccggcgc tccggtcagc gtcggcggcc gaccgatgac
    11941 gctgtccgcg tcggcgagag cctggttcat ggcatcgagc tcggcccggg cctcggcgtc
    12001 gtctccgacc ttggtggcca gggtgtcggc gacggactgc agctcgttgc gggccgtccc
    12061 cagtcggtcc gcggcctcga tgacccggga ctgcgggacc cggagggcgt agatgcggtg
    12121 ctcgaacagg accttctgcg cgtcggcccg gagggaggcg acgtcggggt agcaggcggt
    12181 ggcgatctgc tggccgagcg tggcgagccc gttggccgag ctctgcaggg cgacgatctg
    12241 gccgccgttc accccgtagg gatccgatgc ggcctgcagc gaggcgacga gctcgacgat
    12301 ggtggcctgg cgccgggtga tctcgcccgc gatccgctca cgggcgtggg tgaacggctt
    12361 gcacgcctcc gatgtcgtcg gcggagcgga tcgggagtcc tcggctcccg ccgcggcggg
    12421 tagcgcgacg ggcacgaggg cgctgagccc gacgatcccg acagccagag cacggcggcg
    12481 tcgggtcggg gtggtcggcg aggcggtggt gttcggggtg gtggtcatgg gatggtccct
    12541 tcgttgtcgg tgttgagagc gtcggtggcc tggcgggtgg cagtgtctgc ggcgtcgagg
    12601 gagcggccgg cgccgtcgat gcgggcatcc aggtcggcca gcgagtcagc ggaggcggtg
    12661 tcggcgccgt cgacggtcgc gggcgcggcg gtcgaggtgg gcgcggtgct cggcgcgggt
    12721 ggctcgtcga ccgtcgtggt ctcggcggcg ttccgggaca cggaggcggg cgtggacccg
    12781 tcgtcggcgc gggcattgcc gcatccggct ccgagcgcca cgaccgccac cgctcccagg
    12841 gttcccgcgg agagtcggac gatgcgggct gcggcgcttc gtcggggtcg gtgtcggccc
    12901 ggtcggtcgt cgtgatccat gaccgcagca tcgacgtccg gcgtcagggg gcggtcagcg
    12961 agatgtgagc gtccgatgag ggccggtgcc agaatccggg tgtctcgaag ggggggacgt
    13021 cgtgacggtc accgacaccg atcagctccg ctaccggttc cggggagggg agacttggcg
    13081 cgaaccgtgg gacgactacc gctggttgcg cgaccacgat ccggtccacc gggtcgacca
    13141 cccgacccag ggcacgttct gggtcctgtc gcgcttcgac gacgtgttcg acgcggtgcg
    13201 cgacaccgcc accttcagct ccgcccaggg cctcaccccc gactcagacg tgatggggtt
    13261 cttcgagggg caggccgcgc ctctcgtcat gctggacccg ccggagcaca ccgccatgcg
    13321 ccgattggtg tcgcgtccga tgacgccgag ccgggtcgac gccctggctc cgacgatcgc
    13381 cgcgttcgtc gacgagcgcc tgggccgact cgccgagctc agcggaggag gttctccggt
    13441 cgacgtcgtg gaggtgctgc tcaagccgct gcccagcatg ctggtggcgc acttcctggg
    13501 gatccccgaa caggaccggg gccgattcga cggctggacc gacgcgatcg tcgccgccaa
    13561 ctcacgggag gacctcggct ccgcgatgca cgcgttcgcc gagctgttcg cctactccga
    13621 gtccctgatc gaacgtcggc gaacggaccc gggcgacgac gtcgtgtcgg acctggtgac
    13681 ggcggacgtg gatccccgat ggatcaccgg gttcgtgttc acgatgatcg ccggcggcaa
    13741 cgacaccacc accggcctgc tcggcgggtc cctggagctg ttgaccgcgc gtcgggacca
    13801 gcggctcctc ctgatcgagg acctgtccct cgtccgcccc gcggtcgacg agctgctgcg
    13861 tctgaccagc ccggtgcaga acctggcccg caccgccacc gtcgaggtgg agcgagccgg
    13921 ggtgcgcatc ccggcgggag ccaaggtgat gctgctgtac gggtcggcca accgggacga
    13981 gcggcagtac ggcccgaccg cggacgaact cgacgtcacg cggaacccgg agcggatcct
    14041 gagcttcggc tacggcgcgc accactgtct gggggcggcg gtcgcccggc tggccgcgac
    14101 gatcaccctc gagcggatcc tggagcggtt cccggacttc gtcgtcgacg ccgcctcggg
    14161 tcgcttcgct ccggggccgt acgtgcggcg ctacgagacc ctgccgttcc tcggagtgcc
    14221 gtgaccacgg gcggctgacc gccgcccccg agttggcgtg cgcgtcacga gtccttggcg
    14281 tacatctcgg cggtgtggct ctcgaactcg agtgccacga agggctggtt gccgtcgttc
    14341 ctggcgtcgt ggccgggctc gatcacgtac acgttgccgg cggtgagcac catctcggtg
    14401 ccgtcgtcgt gggtgaccgt cagcgatccc gactggacga cgccgacgtg gcgggtctga
    14461 caggagtcgg ttccggcgat gggtttgatg cactccgacc aggtccatcc cggctgagcg
    14521 gtcagccggg ccaccttggc ggtgccgagg tcgacgatgt ccatctgggt cttgtccggg
    14581 cggcgggtct cgtcggggtc gtcgaacgtc ctcatcgtga tggaagccat gctccacctc
    14641 ctgtgtcccc tgaaccctcg cgcctgatgt ggcgattgtc acgggcgacc gacgtgatca
    14701 gatcccggtt ctcaggaccg ggctcacctg gctcgcccgg ctcggtcggt acgctcgcgc
    14761 ggctgacgcg acggccgctt cagcgaggag ggtgccgacg atgcgctatc gcgactgccc
    14821 gacagtggag gtcagccaac gagtcgccgc gtcgcccgat cgtgcatggc gggcggtctg
    14881 cgacatcggc ctgccggcgc ggtgcggggg tgagttgcag gccgcccggt ggctcgatgg
    14941 tgcgaccgaa ccggcggtcg gcgcccgatt cgagggcgtc aaccgcaacg agcacctcgg
    15001 cgagtggacg acgaccagca cggtcgtcga ggtcgaaccg ggccgacggt gggtgtggga
    15061 ggtggccgac gtcgcgtccg ggccctggtc ccgctggggc ttcgaggtgg atccggtgcg
    15121 tgacggcgtc gtgatgcgcc agtgggcgcg gatcgggccg ggtcggtcag gactgagcct
    15181 ggcgatcgac gccatgccgg accgcgaggc ccggatcgtc gaggtccgga tggccgagtt
    15241 ggctgacgcg atgcgcggca acctcgatcg ggtccgcacc gagctcgaag gatgatcgga
    15301 ccgccggtcg acaccgctgc gtggtgagat cggacgctca gccccggtag gttctgacgc
    15361 catgtccacc cagccggaac cgatcgactt cgtcagggag aaggtccgcg acgaccgcgc
    15421 cgcgggtcgg ttcggcgggc gggtccagac gcggttcccg ccggagccga acgggttcct
    15481 gcacgtcgga cacgccaagt ccgtctacct gaacttcggg atcgccgagg agttcgacgg
    15541 catctgccac ctccgcttcg acgacacgaa ccccgtggcc gaggacgtct cctacgtcga
    15601 ggcgatcgtc gaggacctcg cctggctggg cgtcaccccg tcgggtccgc cccggttcac
    15661 ctccgactcg ttcgaccagc tctacctctg ggccgaggac ctgatcaggg ccggactcgc
    15721 ctacgtcgac gaccaggacc aggcgacgat ctcggcccag cgcggcgggt tcggacagcc
    15781 cggcgtcgag agcccgcacc ggaaccgaag cgtcgaggac aacctggagc gcttccgggg
    15841 catgcgcgac ggtcggtact ccgacgggga gtgcgtcctg cgggcccgca tcgacatgca
    15901 ggccgacaac atgtggctgc gcgacccggt gctctaccgg atccgccgag cccatcacca
    15961 ccgcaccggc gaccgctgga tcatctaccc gacctacgac tgggcccacg gtcaggtcga
    16021 cgcgatcgag ggcacgacgc actcgatctg caccctcgag ttcgccgagc accgaccgct
    16081 ctacgactgg tgcctggacc acgtcgagct cccgtcggac cgacccgagc agaccgagtt
    16141 cgcccgtctc aacctgaccc acaccgtgct gtccaagcgg gtgctgcgca cgctggtcga
    16201 ggacgggctg gtcgacggct gggacgacgc ccggatgccg accgtgcggg gtctgcgacg
    16261 ccgcgggtac ccggccgagg cgctgcgggc gttctgcgat cacatctcgg tggccaagac
    16321 gaactcgacg gtcgagatcg agctgctcga gagcttcgtg cgcacccacc acaaccgtca
    16381 tgcgctgcgg cggatggcgg tgctcgaccc gctcaggctg acgatcacca actggcccga
    16441 gggccacgtc gagtggatgg acgtcatcaa caaccccgag gacgacgccg aggggacccg
    16501 ggccgtgccg ttcaccggcg agctgtggat cgagcgtgac gacttcatgc tggacccccc
    16561 gcccaagtac ttccggctga cgcccggtcg cgaggtccgg cttcgcaacg ccttcttcgt
    16621 caccgccacc gacgtggtgc tcgacgacga cgggaacgtc gtcgaggtgc tcgccaccta
    16681 cgacccggag agcaggggag gggcggcccc cgacggtcgc aaggtcaagt cgactctgca
    16741 ctgggtgtcg cgcccgcacg cggcggacgc gaccgtccac ctctacgagc ggctgttccg
    16801 ctcggcgcac ccgggtgccg acggggtcga cccgctcgag gacctggacc gcgggtcgcg
    16861 gaccacccgg tcggccaagg tcgagccggc cctggccgag gcggatcccg gccaggtcgt
    16921 gcagttcgag cgcctcggct acttcgctgc ggatctggac gacccgtcgg tgttccaccg
    16981 caccgtcggc ctgcgcgacg agtgggccaa catccagaag cagcgcacca cctgactccg
    17041 cggcggcccg accccgcttt gtcccgcaca ggcgcggtga tcaccgcgcc tgtgcgggac
    17101 aaagtcgggc atcatgggcc cgtgatcgat cggctcggag ctctcgacgc cgagttcctc
    17161 cacgtcgagg acggcgtcgg ccacatgcac atcgccggcg cctgcacctt cgatggacct
    17221 ccacccacgt tcgacgacat cgagcagatg gtccgctcga agatgcacct gatcccgcgc
    17281 taccgccagc gggtccgctc ggtccccttc gagctcggtc gtccgatctg ggtcgacgac
    17341 ccgcacttcg acctccgcta ccacctccgc cacaccgcgc tgcccgcgcc cggcgacgat
    17401 gccgcactgt gtcgcttgat gggacgggtg atgtcccagc ccctcgaccg ggaccgaccg
    17461 ttgtgggaga cgtggttggt cgagggtctc gagggcggtc ggtgggcgct ggtgttcaag
    17521 gtccaccact gcatggtcga cggcatctcc ggtgtgggtc tgctcaccgt cctgctggac
    17581 atcggtccgg agtcggaccc cgtcgaggag caaccgtggt cgccaaccgc tgagcccggc
    17641 gttgcccgca aggtgctggg gacatggagc gggctggcag ccgacctctc cacgcaggcc
    17701 catcgggtcg gatctgctct ggcccatccg gtggatgccg ggcgcgacgt cgtgggcctg
    17761 gtcgccggaa ccgcccggtt gggtcgtgcg ttgacggcca ccccacagtc gcccatcgac
    17821 gggtcgatcg ggccccaccg cacctgggcg ttctcgtcgg catcgttcga cgacgtgcgg
    17881 gcagtgcgcg ccgagtgtgg cggcacggtc aacgacatcg tgctgaccgc ggtgaccgcc
    17941 ggctacgcgg cgctgctgcg tcaccgctcc gaggacctgg accgagcggt ggtccgcacg
    18001 ttggtgcccg tctccacgcg gcccaccagc ggtgcgggcg tgcccgacaa ccgggtgtcg
    18061 ttgatgctgc tcgagctgcc ggtgggggtg gcggaccacc tcgaccgcct gcgcgtcatc
    18121 cagggtcgga tgaccgagtt gaaggcctcc cacatggtcg aggtcgggga gctcgccacc
    18181 actctgggcg accttgcccc gccgatggtg atcgggcctg ccagccggtg gctggtcggg
    18241 ctcctgcgtc gggtccccca gcgatccgtc aacacggtga cgaccaacgt tcccggtccc
    18301 cagttcccgc tctactgcct ggggcgggag atgctgtcgt acctgccgta cgtcccgatc
    18361 acccacgggc ttcgggtcgg caccgccatc ctctcctaca acggtcggct gttcttcggt
    18421 gtcaccggtg acgcggcgac cacccccgac gtcgacgtcc tggccgacgc gacggcggcc
    18481 gcggtggggg agatgcgcga tcaggtcggt gcgaccgcca cgcgctgatc tggattttcc
    18541 tcgtcggtac gacgtcggtg gaatcgagta gtgccgcggc cgggggcccg ggcggtaccg
    18601 cgacgacggc tcggaccggt ggtggttccg gcaatgtcag atggacggtg ggtccagggt
    18661 caggcggcgg tgaggaggtg gtggagcagt tcgtcgagcg cgtacgacgt gaggtgttcg
    18721 ccgccgccgt ggcagcgaca cgagtcgggc ccgatgcccg gtggcggctc cggtggttgc
    18781 gtctggagtg gcagcacggt gacatgcacg cggaagccga agaatcccct gcagttgcag
    18841 gggattctct ggagcacccc caacgggatt cgaacccgtg ccgccacctt gagagcgacc
    18901 cttgggccga cttctacgga tggtgacggg cgtagacgac tgcaatttca ggggtttcgc
    18961 gatgaccgga tgtcagcgaa cgaacccgaa tcgcagagat tgtcccgttc gctgttgaag
    19021 tcgccgcggg tggtggtccc ggtgttcatg
//
LOCUS       DAOQOD010000045        18982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1020_length_18982_cov_11.685687, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000045 DAOQOD010000000
VERSION     DAOQOD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18982
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1020_length_18982_cov_11.685687"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLZ72_06360"
     CDS             complement(<1..182)
                     /locus_tag="PLZ72_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35954.1"
                     /translation="MSTGLSASRRPGPLVTIGLLAAVTLSVALGACSGGSGGSSAEKS
                     TTTTEVTTTTFGLPAGA"
     gene            complement(179..1123)
                     /locus_tag="PLZ72_06365"
     CDS             complement(179..1123)
                     /locus_tag="PLZ72_06365"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HNA35955.1"
                     /translation="MLSKALGFLGRAFIIVGLFILGFVAFQLWGTSLEEGRNQSELTD
                     QLAHSVRNTAKVSSDGDAGKLANALAKAFENTDPATAPPTPAPAEGEPVGVIQIPKIG
                     LERVIVQGVSKKDLKKGPGHYPGTPLPGQAGNSGIAGHRTTYGAPFNRIDELAPGDEI
                     NITTPQGRFLYKVIKAPDSDAAPYIVKPTDVTVLDDKGDNRITLTACHPEYSARQRII
                     VNAVLSEEPAPTSPPSKEVTEAVTTSNRALDEGMSGDDSALLPAIAFALAALLVGIAA
                     WFIGTRWKKWPMWLLGTPIVLGLVWFSYVYLDRYLPSL"
     gene            1251..>1891
                     /gene="ccmA"
                     /locus_tag="PLZ72_06370"
     CDS             1251..>1891
                     /gene="ccmA"
                     /locus_tag="PLZ72_06370"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNA35956.1"
                     /translation="MELAVHLRDAVALLGRFPALAGADLDVARGEVVLLRGPNGAGKT
                     TLLRVCAGLAAVSSGVAEVLGVDLVRHRSAIRRRVGLLGHATYLYDDLTVTENVDFWA
                     RAAGAEVADGRRALDRLEVASRLHDLPVHALSAGQRRRVSFAAMVARRPELWLLDEPH
                     AGLDASGRDIIDGLVREAASVGATVLFASHELDRADDVADRTVEVVGGTVVAAP"
     assembly_gap    1892..1991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1992..2667
                     /locus_tag="PLZ72_06375"
     CDS             <1992..2667
                     /locus_tag="PLZ72_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNA35957.1"
                     /translation="SRPMRDAVLVMTKDLRLEWRSKVGIGQVLPFSLLVVLLFAFALD
                     PERGVLDRVTSGLFWVVVLFSSVLTIERAFSVEAADGIFDALRLSGLAPAAIYAGKVA
                     ALAVQLLVVEAVLAVAVAVFYDTTFRTPVLTVGVALVATVGIAAAGAVYGALAARLRS
                     GTTLLPLLLLPLLAPVLIGATRGFEVALGREAGPGWPWVALLGIFAVVYLTLGALLFR
                     PLLEDA"
     gene            2664..3473
                     /gene="ccsA"
                     /locus_tag="PLZ72_06380"
     CDS             2664..3473
                     /gene="ccsA"
                     /locus_tag="PLZ72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNA35958.1"
                     /translation="MTVDNPTTGAGRPPGAASEPPTGPPTTGSTATRVLGVITLVGIA
                     ALVLLGVFLTPADAELGDTIRILYMHVPTVSTAYLAFVITAIASVMYLWKRTEFWDLF
                     AASSAELGVLFFALTILNGMLWGKITWGVFWRWEPRLTTTAVLMLTYVGYLAVRQIPN
                     DTRTRGTISAVIGIMAVVNIPITHKAVDWWRGLHQEKTIFGTVDPDMKGLQLFTAYLG
                     LTVFLVLFAWLLIHRFRVAWLAERAADVGVDAAIAERRAERAVPSSAGGAS"
     gene            3470..3604
                     /locus_tag="PLZ72_06385"
     CDS             3470..3604
                     /locus_tag="PLZ72_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35959.1"
                     /translation="MSDTAYIATAWIATFGSLGLYSLWLLRRGRKLSRSVPPDERRWL
                     "
     gene            3595..4245
                     /locus_tag="PLZ72_06390"
     CDS             3595..4245
                     /locus_tag="PLZ72_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNA35960.1"
                     /translation="MAVTPPDPDGMHLDDPESADATTGPAESDDRLDVTPRTAPDRRP
                     GGSGSSRTRTYVAVGVVAVIVVGLGLVLVNGLRDASTFFYNVDEVAAKHDELQGERIR
                     VQGNVVDGTVRKTDDGVSFVLRFRGEELPVDHVGDPPELFGPKIPVVLEGTLRGDTFH
                     SDTILVRHDATYDEENHDRVREAEKDAVTTTAPGGPAAQDVTETGPGGPALGGASG"
     gene            4242..6269
                     /locus_tag="PLZ72_06395"
     CDS             4242..6269
                     /locus_tag="PLZ72_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNA35961.1"
                     /translation="MNRALGLAGVIVGFGGALLGIIVLATGLRTRRESLLRMGRSYAG
                     IVLAGAVLACFAMQRALITRDYTVRYVFEHGSSRTPPLFNVATMWSALDGSILLWAVI
                     LAGYTYLVAHKFRRRSSDPLVAWALIVMFVVAVFFFGLMLSATNPFRGVVPPPGYDGP
                     GPNPLLQNHILMAFHPPMLYLGYVGFTVPFAFAVAALVTGRVGEGWLVETRRWTLFAW
                     MFLTAGIVLGAWWSYEVLGWGGYWKWDPVENASFLPWLTATAYIHSVMVQERRGMLRV
                     WNLSLLCATFSLTILGTFLTRSGVVESVHAFSDGTVGPLLLGFFALVVAVSVGLIGWR
                     GDRLRSPGRIDSPVSREGAFLANNVVFAAFAFIVLLGTVFPLIVEAVNGDRLSVGVPY
                     FSRMTMPIGFVLLFLMGVAPVLPWRKGTPELLRDRLWWPAWAGVGSVLLALAVGATGW
                     AALVAFGLGGFAAGSAVRQLALATRRQGWRGLLGRANGGMVVHLGAIVIGVAFAASMS
                     YIRQAELTLEPGKTATVAGHSVEYLGSRTIEADNRVELIARVRVDGDRVFEPRLNKYR
                     VDGQTIGTPSVRVGPTDDVYLALTKAPSGEGSAIGLRVIVQPLILWLWVGGIVMVVGT
                     LLALFPGRRRRNPLDPVSAPVVSTLPRRDHEADPGPRPDPDSDPEPEPVPT"
     gene            6266..6841
                     /locus_tag="PLZ72_06400"
     CDS             6266..6841
                     /locus_tag="PLZ72_06400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA35962.1"
                     /translation="MRHRSAIIAAVVGVALAGLVALLAFSPQGGDGAASASPVVGKVA
                     PALVGTTADGQSFDLDKHRGQWVLVNFFATWCPPCVAEHPELVRLAGEQDGTLQVVSV
                     AAGDTAEKVTEFFAEKGGDWPVLVTDTDAASIDYGLIKLPESYLIAPDGTVVEKYIGG
                     ITAEQVEARIARGAGTGAGGPASSTVVGAGS"
     gene            6838..7416
                     /locus_tag="PLZ72_06405"
     CDS             6838..7416
                     /locus_tag="PLZ72_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNA35963.1"
                     /translation="MTAEVSRRAPVRHRYRQQWWWWALMGLIVVVAVVVGAGSPPRSG
                     TSDERLFALAGRLKCLQCVGESVAASQAPLAIQFREEIRDQMRQGGTDDEILNFFADR
                     YGTEVLLTPPSSGVGGLVWVVPVVVVAGAVLLLAGTFRRWSRERSEHHASDEDVALVA
                     DALAAEPRAGSRPVPDPDSAPAPAPEPRSDDR"
     gene            7413..>8152
                     /locus_tag="PLZ72_06410"
     CDS             7413..>8152
                     /locus_tag="PLZ72_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35964.1"
                     /translation="MSTTARQGHLDPDRLAELEEQRDHLLASLRDLEREHDAGDLDDH
                     DYGELKDDYTARAAEVIRAIDEHRDLLEQSRPERNRGRTALVVVSVLAVAVLAGFLLA
                     RSSGQRGSGTITGNDDTLRQRLASCQMTSFQKPAEGVDCYADILDEHPDNLEALTYQG
                     WAFVRDDQVERGAKNLARVVEIDPDYPDARVFRAVLLARAGTAARAKGDDATARQSYT
                     AAAAEVDRFYRNDPPEVAVQVLRQEGLER"
     assembly_gap    8153..8252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8253..8667
                     /locus_tag="PLZ72_06415"
     CDS             <8253..8667
                     /locus_tag="PLZ72_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35965.1"
                     /translation="CLDGVLAADPTSTDALLSKALTLLGTETPDVAAATTLAERILAV
                     EPDNGNALLLLASAAVYRDDLDGATALLDRVDGLPRPTGAALIGSPEDLRSMIERRRS
                     GGSSGPGGATTTTTEPRATASTVPGAPRIPNADGG"
     gene            8770..9987
                     /locus_tag="PLZ72_06420"
     CDS             8770..9987
                     /locus_tag="PLZ72_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNA35966.1"
                     /translation="MRIMLVGAGGVGDAFCAIASHREFVESIVVGDIDPARAERVASR
                     DDRFTATTLDASDPASIAAAATDARADLVMNAADPRFVMPIFDGAFAAGANYMDMAMS
                     ISEPHPDRPHELTGVKLGDRQFAKAEKWTERGLLAVCGMGVEPGMADVFARYAADELF
                     SSIQEVGIRDGADMVVRGYDFAPTFSIWTVIEECLNPPVIWERDRGWYTTPPFSEPEV
                     FRFPEGIGDVECVNVEHEEVLLVPRWIDCERVTFKYGLGDEFIQVLETLHKLGLDRTD
                     PVSVKGSSVSPRDVVAACLPDPAGLGDRIEGKTCAGAWVTGTGKDAEGNATGPRDVYL
                     YHVVDNAETMGRDGAQAVVWQTAVGPVIACELIDAGVWTGAGVVGPEAMSPVPFLDLL
                     GGEYGSPWGIEER"
     gene            complement(9992..10618)
                     /locus_tag="PLZ72_06425"
     CDS             complement(9992..10618)
                     /locus_tag="PLZ72_06425"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA35967.1"
                     /translation="MAPLPDADPPPARRVDPRVVHTRQVVIDAVADLLSTDGLAQLTM
                     DAIAARSGVARSTIYRHWPDRVELMVDVFESVSSVADPLDPCGPLHADLRATGDSLAR
                     GLTEHRWGLILSCLVGTAGHDDEIHRALNTFGRARRAESAEIFSRAITRGEIRSDLDP
                     VCEAERFAGPFFLRRLVTADTLDADFVEAQVRATCRALGAPYEHPPAV"
     gene            10752..12344
                     /locus_tag="PLZ72_06430"
     CDS             10752..12344
                     /locus_tag="PLZ72_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA35968.1"
                     /translation="MTAHPESWIRERRWWILACLCLSLVLVVAGNSSLNVALPKIQAD
                     LGSAQSDLQWMVNAYGLVFAVLLLPAGALADRYGRKSALQLGMVIFGAASFVSIFATE
                     NWHLIALRGVTGLGAAFIMPGTLSILANVFHDPRERQRAIAIWAGFAGLGGAIGPILS
                     GFLLEHYSWGSVFLISVVIAAVALVAGAYLLPNSSDPHEAILDPLGVMLAIAAVGTLL
                     YGIIEGPDNGWTSTITLGSIAAAVVLMGAFVTWELRNPNPMLDVRLFRNRSFSVGSTT
                     ITLQYFAAFGLFFAMAQYLQIAHGYSPLQAGLAGLPIGVMSMIGAPLSATFVRRFGHR
                     GVVGVGLIMSAGGLALLSTASPTTSLALLLVGFCLLGLGAGQSTAPSTTLIMTAVPRS
                     KAGVGSAVNDLSRELGGALGVAVLGSVISSVYRSNIAGHVGPALASTAGTSVNETLAA
                     AQHAASSGSGAAAGALVHGAQMSFADGFGAAMVLGALVLLLNAALVWFRGLDVDPHAG
                     WGSDAGGRPPVNADAPTDPASA"
     gene            complement(12395..12643)
                     /locus_tag="PLZ72_06435"
     CDS             complement(12395..12643)
                     /locus_tag="PLZ72_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35969.1"
                     /translation="MASHTSTGDTIFHRDGRWRVVLLGQGDPDAMFGTLDVLAELEPD
                     VVASSAFDGITGAYELGDRPWASCVAEARDGLQRTLAT"
     gene            complement(12823..13797)
                     /locus_tag="PLZ72_06440"
     CDS             complement(12823..13797)
                     /locus_tag="PLZ72_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA35970.1"
                     /translation="MPAVTADTLTLPRISPAAVDDVERPVANISTGPTGLEGMGFPVR
                     RALAGAPYQLTDPFIMMDHLGEIDYGIGEPKGTDWHPHRGFETVTYLMDGLFQHQDSS
                     GGGGLIEPGDTQWMTAGSGVLHIETPPASMVESGGLFNGVQIWVNLPASAKWTPPAYQ
                     GITKRDILLLTSPDAGAIIRVIAGDVDGHVGPGSTHTPITIIHASIHAGERLSLPWNP
                     SFNAMAYVFAGSGTAGTEEAPIHGGNMVVFGRGDRLTLAADATQDSRTDALEVMLLGG
                     EPIGEPAFQYGPFVMNTEAELAQAIDDYQHGRMGVIPPGALQPHVHRG"
     gene            complement(13810..14715)
                     /locus_tag="PLZ72_06445"
     CDS             complement(13810..14715)
                     /locus_tag="PLZ72_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA35971.1"
                     /translation="MGDYGRDVQFGLFLSPDTAILDQTLATAQAADAAGLELIGIQDH
                     PYQKQFLDTWALMATVLARTERIRVFPDVASLPLRPPAVLAKAAASLDVISGGRFELG
                     LGAGAFWEAIAAMGGPNRSGAEAAEALTEAVEIVRLMWSAQRSVRFTGTHYQIAGVHP
                     GPQPAHDMGIWLGVGGPRMLALVGRAADGWLPSSGFFPPDKLPDMHRRIDDAATDAGR
                     DPSDISRIYNVWGTITDGPSNGEFDGPVDQWIDTLTGLVLDGGMNAFVFGAEGDDPAQ
                     MSAFMDRVVPAVREQVAAGRSGRTP"
     gene            complement(14739..15299)
                     /locus_tag="PLZ72_06450"
     CDS             complement(14739..15299)
                     /locus_tag="PLZ72_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNA35972.1"
                     /translation="MSTTESTTATGTYTIDPAHSRIGFVARHAMVTKVRGSFTEFTGS
                     GSFDEENPANSQLQLTIQANSIDTGNADRDGHLRSNDFFDMETFPEITFASTGVAKTG
                     DDTFEVTGDLTIKGVTRPVTVEFDYTGTAVDPYGNTRLGLEGKTTVNRKDWGVNWNTA
                     LEAGGVLVSEKVTLEFDVSAIRSADA"
     gene            15382..15846
                     /locus_tag="PLZ72_06455"
     CDS             15382..15846
                     /locus_tag="PLZ72_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA35973.1"
                     /translation="MTDEPAWVVWEAVQSMYEQVETELGRRLGSGSQLSCTDYKVLAA
                     LTSHPDGRMRLFELVDALGWEKSRVSHQVSRMTERGLLDKTRCPNDRRGAHVGPTARA
                     RAAMAEAEPAYIDAVQALLGETLTRAQLASLHDAARAVLAGIADPDPVGSQR"
     gene            complement(15881..16741)
                     /locus_tag="PLZ72_06460"
     CDS             complement(15881..16741)
                     /locus_tag="PLZ72_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35974.1"
                     /translation="MKRLSAIGALLVAVSLLGAACTVGGPAPTDWKVKPATIKVVTGE
                     DMDAGDEPYVIQVGFRSKLGVAGSTHVQVASQCRSGALPATDAAPNGTTVAIPAGSAD
                     VTFPQAQNLDIGDVLLGTAPLEIFGTLTFVMERDGILDSCAMTDAFESALVPVMHDAL
                     ETLIASSPVPPTQEQLISLIVDNIGNFLNAVGSFLAAIIEGLGNPDDVIGVGVQILLP
                     TAGAFTDLLNAAFSIGGVFLPGLESGFIPINGLPSSVKIRVGSLLPSAATFDFDGDAA
                     HYVYSSTVGA"
     gene            complement(16890..17354)
                     /locus_tag="PLZ72_06465"
     CDS             complement(16890..17354)
                     /locus_tag="PLZ72_06465"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNA35975.1"
                     /translation="MTLYLVRHGSAGARDDSDPHDDDRPLDPIGHQQAERIRDWLVHE
                     SPVVVRSSPYRRCVQTVDPLAAALGLDVVVDERLAEGTPIEESWALVEELVTTTAVLC
                     SHGDVIPELVSRAQRRGMVVPGKSGCSKGSVWALRHWDDRTFATGLYTPVRV"
     gene            complement(17476..>18982)
                     /locus_tag="PLZ72_06470"
     CDS             complement(17476..>18982)
                     /locus_tag="PLZ72_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNA35976.1"
                     /translation="TAPFWHAGSTTARAAGVIAPDRASGIGAPAVADLDGDGSPEVVV
                     ADLEGSVHVFGADGSVRASMSSDPRFSRGEATDERNRMKRGFVGGAALGDLDGDGAFD
                     VVAAAMDRHVYAWHADGSPLAGFPVLVVDPAQVDDVDADTHHVTFRSPGATGQGGEIV
                     ATPALGDLDGDGRPEIVIGTQEQYDEPPAVFPGLGLPGQSGNTRLFAISSYGTGHPAG
                     ADRSAAHPDDRAYLAGWPVALPMFLTAVLPTIGDGVATQAAIGDVDSDGAVEVVATSV
                     SGQTMVLDADGRSPYGRPFGVQLALNWLGAVPPGTDSSDTAALTSAFGGAAIGRLDPD
                     VPDGGSGPGLDVVVPTTGAGRALDTLFANDQPGDPQLTAWSGRDGAIRPGFPRVTADV
                     AFFVTPAIADVDGDGSDEVVAANGVQMLDAFRGDGTPPSGWPKLTGGWAVGTPAFGDW
                     DGDGRGDVAITRRDGHLLVWRVPTRADALGDWIRFGGNDRNDGTPSLGSNG"
BASE COUNT         2548 a         6477 c         6794 g         2963 t
ORIGIN      
        1 gctccggccg ggagcccgaa ggtcgtcgtg gtcacctcgg tggtggtggt cgacttctcg
       61 gcgctggaac cgccggagcc gcccgagcag gcgcccaggg ccaccgacag cgtcaccgcg
      121 gcgagaagac cgatcgtgac gagcggcccg ggccgacggg atgcggatag gccggtgctc
      181 acagcgacgg caggtaccgg tccaggtaca cgtagctgaa ccacaccagg ccgagcacga
      241 tcggcgtgcc cagaagccac atcggccact tcttccagcg tgtgccgatg aaccacgctg
      301 cgatcccgac cagcagcgcc gcgagagcga aggcgatcgc gggaagcagg gccgagtcgt
      361 cgccggacat gccctcgtcg agggcgcggt tcgaggtggt caccgcctcg gtcacctcct
      421 tggatggcgg cgaggtgggc gccggctcct cggacagcac ggcgttgacg atgatccgct
      481 gacgggccga gtactcggga tgacaggccg tcagcgtgat gcggttgtcg cccttgtcgt
      541 cgagcacggt cacgtcggtg ggcttgacga tgtagggcgc cgcgtcggag tcgggagcct
      601 tgatcacctt gtagaggaat cggccctgcg gggtggtgat gttgatctcg tcgccgggag
      661 ccaactcgtc gatccggttg aacggcgcgc cgtaggtggt tcggtgcccg gcgatgccgg
      721 agttgccggc ctggcccgga agcggggtgc ccgggtagtg gcccggcccc ttcttcaggt
      781 ccttcttgga gaccccctgg acgatgacgc gctccaggcc gatcttgggg atctggatca
      841 cgccgaccgg ctcgccctcg gcgggggccg gtgtgggcgg cgcggtggcc gggtcggtgt
      901 tctcgaacgc cttggccagc gcgttggcga gcttgccggc gtcgccgtcg gaggacacct
      961 tggcggtgtt gcgcaccgag tgggcgagct ggtcggtgag ctcggactgg ttgcggccct
     1021 cctccaggct ggtgccccag agctggaacg cgacgaagcc caggatgaac aggccgacga
     1081 tgatgaacgc ccggccgagg aaccccagag ccttcgacag cacctcacca ccgtatcggg
     1141 gcttggcccg cgccccgacg cgaccggtag cgttgccggg tccccgaggg tgctctccgc
     1201 tgtggcgcgg cgctccctcc gccctccgat ccgctcggaa gtcctgtccc ttggagctcg
     1261 cagtccacct ccgtgatgcc gtcgccctcc tcggccggtt cccggccctg gccggggccg
     1321 atctggacgt ggcgcggggc gaggtcgtac tgctgcgggg ccccaacggc gccggcaaga
     1381 ccaccctgct gcgggtgtgc gccggtctgg ccgcggtgag ctcgggggtg gccgaggtcc
     1441 tgggggtgga cctggtccgc caccgctccg cgatccgtcg ccgggtcggt ctgctcggcc
     1501 acgccacgta cctctacgac gacctgaccg tgaccgagaa cgtcgacttc tgggcccggg
     1561 ccgcgggcgc cgaggtcgcg gatgggcgcc gggccctcga ccggctcgag gtggcgtccc
     1621 ggttgcacga cctgccggtg cacgccctgt cggccggtca gcgtcggcgg gtgtcgttcg
     1681 cggcgatggt ggcccggcga cccgagctgt ggctgctcga cgaaccgcac gccgggctcg
     1741 acgcgtcggg gcgcgacatc atcgacggtc tcgtccgcga ggcggcgtcg gtgggtgcga
     1801 cggtgctgtt cgcgtcccac gagctcgatc gggccgacga cgtggcggac cggaccgtgg
     1861 aggtcgtcgg cgggacggtg gtcgcagcgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn ngtcgcgtcc gatgcgtgac gcggtgctgg tcatgaccaa ggacctccgc
     2041 ctcgagtggc gctccaaggt cggcatcgga caggtgctcc cgttctcgct gctggtcgtg
     2101 ctgctgttcg ccttcgctct ggaccccgag cgtggcgtgc tcgaccgtgt cacctccggg
     2161 ttgttctggg tggtcgtcct gttctcgtcg gtgctcacca tcgagcgtgc gttctcggtc
     2221 gaggcggccg acgggatctt cgacgccctg cgcctgtcgg gtctggcccc cgccgccatc
     2281 tacgccggca aggtcgcggc tctggccgtc cagctcctgg tggtcgaggc ggtgctcgcg
     2341 gtggcggtcg cggtcttcta cgacacgacg ttccgcacgc cggtgctcac cgtcggcgtc
     2401 gccctggtcg ccaccgtcgg catcgccgcg gccggcgcgg tgtacggggc gctggcggcc
     2461 cggctgcgca gcgggaccac gctgctgccg ttgctcctgc tgccgctgct cgctccggtg
     2521 ctgatcggcg ccacccgcgg cttcgaagtg gctctggggc gcgaagctgg tccgggttgg
     2581 ccctgggtcg ccctgttggg catattcgcc gtggtctacc tcacgctcgg agcgctcctg
     2641 ttccggcccc tcctggagga cgcatgacag tcgacaaccc gacgaccggc gcgggccggc
     2701 ccccgggcgc cgcatcggaa ccaccgacgg gtccgcccac gaccgggtcg accgccaccc
     2761 gggtgctcgg cgtgatcacc ctggtgggca tcgccgcgct ggtcctgctc ggcgtgttcc
     2821 tcaccccggc cgacgccgag ctgggcgaca cgatccgcat cctgtacatg cacgtgccca
     2881 cggtctcgac cgcatacctg gccttcgtga tcacggcgat cgcctcggtg atgtacctgt
     2941 ggaagcgcac cgagttctgg gacctgttcg ccgcgtcgtc ggccgagctc ggcgtgctgt
     3001 tcttcgccct caccatcctc aacgggatgc tgtgggggaa gatcacctgg ggggtgttct
     3061 ggcgctggga gccgcggctg accaccacgg cggtgctcat gttgacctac gtcggctacc
     3121 tggcggtccg tcagatcccc aacgacacgc gcacccgcgg cacgatcagc gccgtgatcg
     3181 gcatcatggc ggtcgtcaac atcccgatca cccacaaggc ggtcgactgg tggcggggcc
     3241 tgcaccagga gaagacgatc ttcggcaccg tcgaccccga catgaagggc ctgcagctgt
     3301 tcaccgccta cctgggcctg accgtgttcc tcgtgctgtt cgcctggctg ctgatccacc
     3361 gcttccgggt ggcgtggctg gccgagcggg ccgctgacgt cggcgtcgac gccgccatcg
     3421 cggagcgccg ggccgagcgg gccgtcccgt cgagcgccgg aggtgcgtca tgagcgacac
     3481 cgcgtacatc gcgacggcct ggatcgcgac attcggatcg ctcgggctct actcgctgtg
     3541 gctgctgcgg cgcgggcgca agctgtcgcg ctcggttccg cccgacgagc ggaggtggct
     3601 gtgaccccgc ccgatcccga cgggatgcat ctcgacgatc ccgagtccgc cgacgcgacc
     3661 acgggaccgg ccgagagcga cgaccggctc gacgtcaccc ctcgaaccgc tcccgaccga
     3721 cgcccgggcg ggtcgggttc gtcgcggacc cggacctacg tggcggtcgg cgtcgtcgcc
     3781 gtgatcgtgg tcgggctcgg cctggtgctg gtcaacggcc tccgcgacgc gtcgacgttc
     3841 ttctacaacg tcgacgaggt cgccgcgaag cacgacgagc tgcagggcga gcgcatccgc
     3901 gtgcagggca acgtcgtcga cggaacggtg cgcaagaccg acgacggcgt gagcttcgtg
     3961 ctgcgcttcc ggggcgagga gctgcccgtc gaccacgtcg gtgacccgcc cgaactgttc
     4021 ggccccaaga tccccgtggt gctcgagggc accctccgcg gcgacacgtt ccacagcgac
     4081 acgatcctgg tccgccacga cgcgacctac gacgaggaga accacgaccg ggtccgggag
     4141 gccgagaagg acgcggtgac cacgaccgca ccggggggac ccgccgcgca ggacgtgacc
     4201 gagaccggcc cggggggacc ggcgctgggg ggtgcctccg ggtgaaccgg gccctcggac
     4261 tggccggggt catcgtcgga ttcggcgggg cgctcctcgg catcatcgtt ctggccaccg
     4321 gcctgcggac gcgacgcgag tcgctgctgc gcatgggccg ctcctacgcc ggcatcgtgc
     4381 tggccggggc ggtgctggcc tgcttcgcca tgcagcgggc gctgatcacc cgcgactaca
     4441 ccgtccgcta cgtgttcgag cacggctcgt cacggacccc gccgctgttc aacgtggcca
     4501 ccatgtggtc cgctctcgac gggtcgatcc tgctgtgggc ggtgatcctg gccggctaca
     4561 cgtacctggt cgcccacaag ttccgtaggc gttcctcgga cccgctggtg gcgtgggctc
     4621 tcatcgtcat gttcgtggtg gcggtgttct tcttcggcct gatgctcagc gccaccaacc
     4681 cgttccgcgg ggtggtcccg ccgcccggct acgacggccc gggacccaac ccgctgctgc
     4741 agaaccacat cctgatggcg ttccatccgc cgatgctcta cctgggctac gtcggtttca
     4801 ccgtcccgtt cgcgttcgcc gtggcggcgc tggtgacggg tcgggtgggg gaggggtggc
     4861 tggtcgagac ccggcgctgg acgctgttcg cctggatgtt cctcaccgcc ggcatcgtgc
     4921 tgggcgcctg gtggagctac gaggtgctcg gctggggcgg gtactggaag tgggacccgg
     4981 tcgagaacgc gtcgttcctt ccgtggctga ccgcgacggc ctacatccac tcggtgatgg
     5041 tccaggagcg ccgggggatg ctgcgggtct ggaacctgtc gctgttgtgc gccacgttct
     5101 cgctgacgat cctgggcacg ttcctgaccc gatccggggt ggtcgagtcg gtccacgcgt
     5161 tctccgacgg caccgtcggt ccgttgctgc tcgggttctt cgcactggtc gtcgcggtgt
     5221 cggtcgggct gattggctgg cggggcgacc gcctccgctc acccgggcgc atcgactcgc
     5281 cggtgtcacg cgagggcgcc ttcctggcca acaacgtcgt gttcgcggcg ttcgcgttca
     5341 tcgtcctgct cggcacggtg ttccccctga tcgtcgaagc ggtcaacggg gaccggctgt
     5401 cggtcggcgt cccgtacttc agccgcatga cgatgccgat cggcttcgtg ctcctgttcc
     5461 tgatgggggt cgcacccgtg ctcccgtggc gcaaggggac gcccgagctg ctgcgcgacc
     5521 ggttgtggtg gccggcgtgg gccggggtcg gttccgtcct cctcgccctc gccgtcggcg
     5581 ccaccgggtg ggcggcgctg gtggcgttcg gcctgggtgg gttcgcggcc ggctccgcgg
     5641 tccgtcagtt ggcgctcgcc acccgacgcc aggggtggcg ggggctgctc ggccgggcca
     5701 acggcgggat ggtcgtgcac ctcggcgcca tcgtgatcgg tgtcgccttc gcggcgtcga
     5761 tgagctacat ccgccaggcc gagctcaccc tcgagccggg caagaccgcg acggtggccg
     5821 gccacagcgt cgagtacctc gggtcgcgca cgatcgaggc cgacaaccgc gtcgagctga
     5881 tcgcccgggt gcgcgtcgac ggcgaccggg tcttcgaacc ccgtctgaac aagtaccgcg
     5941 tcgacggcca gacgatcggc accccgtcgg tgcgggtcgg gccgaccgac gacgtctacc
     6001 tggcgttgac caaggcgccg tcgggtgagg gctccgccat cgggctgcgc gtcatcgtgc
     6061 agcccctgat cctgtggttg tgggtcggcg ggatcgtgat ggtggtcggg acgttgctcg
     6121 ccctcttccc gggccgtcgg cgtcgcaacc cgctcgaccc ggtgtccgcg cccgtcgtct
     6181 cgacccttcc tcgacgcgat cacgaggcgg atcccggccc ccgacccgat cccgactccg
     6241 accccgaacc cgagccggtt ccgacgtgag gcaccgctcc gcgatcatcg ccgccgtcgt
     6301 cggggtggcc ctggccggcc tcgtagccct cctggcgttc agtccgcaag gcggcgacgg
     6361 cgccgcgtct gccagcccgg tggtcggcaa ggtcgcaccc gcgctggtcg gcaccaccgc
     6421 ggacgggcag agcttcgacc tggacaagca ccgcgggcag tgggtgctgg tcaacttctt
     6481 cgccacgtgg tgcccgccgt gcgtggccga gcaccccgag ctggtccgcc tggccggcga
     6541 gcaggacggc accctgcagg tggtgagcgt ggccgccggc gacaccgccg agaaggtcac
     6601 cgagttcttc gccgagaagg gcggggactg gccggtgctg gtcacagaca ccgacgccgc
     6661 ctcgatcgac tacgggctga tcaagctgcc cgagtcgtac ctcatcgccc ccgacggcac
     6721 cgtggtcgag aagtacatcg gcgggatcac cgccgaacag gtcgaggccc gcatcgcccg
     6781 gggcgcgggc acgggcgcgg gtggtccggc gtcctcgacc gtcgtgggag ccggctcgtg
     6841 accgccgagg tctcccgacg ggcaccggtg cgccaccggt accgccagca gtggtggtgg
     6901 tgggcgctga tgggtctcat cgtggtggtc gccgtcgtcg tcggcgccgg ctcgccaccg
     6961 cgttcgggca cctctgacga gcgactcttc gccctcgccg gccgcctcaa gtgcctccag
     7021 tgcgtcggcg agtccgtcgc cgcgtcccag gctccgctcg cgatccagtt ccgcgaggag
     7081 atccgcgacc agatgcgcca gggcggcacc gatgacgaga tcctgaactt cttcgccgac
     7141 cgctacggca ccgaggtcct cctgaccccg ccgtcgagcg gtgtcggcgg gctggtgtgg
     7201 gtcgtcccgg tggtcgtggt ggccggagcg gtcctgctcc tggcggggac cttccggcga
     7261 tggagccggg agcgatcgga gcaccacgcg tcggacgagg acgtcgccct ggtcgccgac
     7321 gccctcgcgg ccgagccacg ggccgggtcc cggcccgtgc cggatccaga ctcggctccg
     7381 gctccggctc cggagccgcg atcggatgac cggtgagcac cacggcacgt cagggccacc
     7441 tcgaccccga ccgcctggcc gagctcgagg agcagcgcga ccacctgctg gcgtcgctgc
     7501 gcgacctcga gcgcgagcac gacgccggcg acctcgacga ccacgactac ggcgagctga
     7561 aggacgacta caccgcccgg gccgccgagg tgatcagggc catcgacgag caccgcgacc
     7621 tgctcgagca gagccgaccg gagcggaacc gtggtcgcac cgcgctcgtc gtcgtgtcgg
     7681 tgctcgcggt ggcggtcctg gccggcttcc tgctggcccg ctcgtcgggc caacgcggca
     7741 gcgggacgat caccggcaac gatgacacgc tgcgccaacg cctggcgtca tgtcagatga
     7801 cgagcttcca gaagccagct gagggcgtcg actgctacgc cgacatcctc gacgagcacc
     7861 ccgacaacct ggaggcgttg acctaccagg ggtgggcgtt cgtccgtgac gaccaggtgg
     7921 agcggggggc gaagaacctg gctcgggtgg tcgagatcga tcccgactat cccgacgccc
     7981 gagtcttcag ggcggtgctg ctcgcccggg ccggaaccgc ggctcgggcc aagggagacg
     8041 acgcgaccgc ccgtcagagc tacaccgcgg cggcggccga ggtcgaccgc ttctaccgca
     8101 acgacccgcc cgaggtcgcg gtgcaggtcc ttcgtcagga gggcctcgaa cgnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgcctcg acggagtcct ggccgcggac
     8281 ccgacctcga ccgacgcact gctgtccaag gcgctcaccc tgctcggcac cgagaccccc
     8341 gacgtggccg cggcgacgac gctggccgag cgcatcctgg ctgtggaacc cgacaacggc
     8401 aacgccctgc tgctgctggc gtcggccgcg gtgtaccgcg acgatctcga cggcgcgacg
     8461 gcgctgctcg accgggtcga cggacttccc cgaccgaccg gcgccgcgct gatcgggtcc
     8521 cccgaggacc tgcggtcgat gatcgaacgc aggcgctcgg gaggatcgtc gggtccggga
     8581 ggggcgacga ccacgaccac cgagccccgc gccaccgcgt cgaccgttcc gggcgcgccg
     8641 cgcatcccca acgccgacgg cggctgaccc gccccctgac ccgccccccc accccgttct
     8701 gttcgttgtt tcacaacgct gacgttgtga aacaacgaac agaacggggt ggtcggtagc
     8761 ctgccggcca tgaggattat gctggtcgga gcgggagggg tcggcgacgc gttctgcgcg
     8821 atcgcatcgc accgcgagtt cgtcgagtcg atcgtcgtgg gcgacatcga cccggcccgg
     8881 gccgaacggg tcgcgtctcg cgacgaccgg ttcaccgcca ccaccctcga cgcgtcggac
     8941 ccggcgtcga tcgccgctgc cgccaccgac gcccgcgccg atctggtcat gaacgcggcc
     9001 gacccccggt tcgtcatgcc gatcttcgac ggcgcgttcg ccgcgggtgc gaactacatg
     9061 gacatggcca tgtcgatctc ggagccgcac ccggaccggc cccacgagct caccggcgtc
     9121 aagctcggcg accgccagtt cgccaaggcc gaaaagtgga ccgagcgggg gctgctcgcg
     9181 gtgtgcggca tgggggtgga gcccggcatg gccgacgtgt tcgcccgcta cgccgccgac
     9241 gagctcttct cctcgatcca ggaggtgggc atccgcgacg gggccgacat ggtggtgcgc
     9301 ggctacgact tcgcaccgac cttctcgatc tggaccgtca tcgaggagtg cctgaacccg
     9361 ccggtgatct gggagcgcga ccgcggctgg tacaccacac cgccgttctc cgaacccgag
     9421 gtgttccggt tccccgaggg catcggcgac gtcgagtgcg tcaacgtcga acacgaagag
     9481 gtcctgctgg tcccgcgctg gatcgactgc gagcgggtca ccttcaagta cggcttgggc
     9541 gacgagttca tccaggtgct cgaaacgctc cacaagctcg gcctcgaccg caccgacccg
     9601 gtctcggtca aggggtcctc ggtgtcacct cgtgacgtgg tggcggcctg ccttcccgat
     9661 ccggccggcc tcggggaccg catcgagggc aagacctgcg ccggggcgtg ggtgaccggg
     9721 acggggaagg acgccgaggg caacgccacc ggtccgcgtg acgtctacct gtaccacgtg
     9781 gtcgacaacg ccgagaccat gggtcgcgac ggcgcccagg ccgtcgtgtg gcagaccgcc
     9841 gtcggtccgg tgatcgcgtg cgagctgatc gacgccgggg tgtggaccgg tgccggagtc
     9901 gtcggacccg aggccatgtc accggtcccg ttcctggacc tgctcggcgg cgagtacggc
     9961 tcgccgtggg ggatcgagga gcgctgaggg atcagaccgc tggggggtgt tcgtagggtg
    10021 cgccgagcgc acggcacgtc gctcggacct gtgcttcgac gaagtcggcg tcgagcgtgt
    10081 cggcggtgac gagtcgccgc aggaagaacg gccccgcgaa gcgttcggct tcgcataccg
    10141 ggtcgagatc gctcctgatc tcacctcggg tgatcgctcg ggagaagatc tctgcggact
    10201 cggctcgacg ggcccgaccg aaggtgttga gggctcggtg gatctcgtcg tcgtggcccg
    10261 cggtcccgac cagacacgag aggatcagcc cccacctgtg ctcggtcaga ccgcgggcca
    10321 ggctgtcgcc ggtggcgcgc aggtcggcgt ggagcggccc gcacggatcg aggggatcgg
    10381 ccaccgagct gacggactcg aacacgtcga ccatgagctc cactcgatcg ggccagtgcc
    10441 gatagatggt cgatcgggcg acgcccgagc gggcggcgat cgcgtccatc gtgagctgcg
    10501 cgaggccgtc ggtggagagc aggtccgcaa ctgcgtcgat gacgacctga cgggtgtgga
    10561 ccacccgggg atcgacacgc cgagcgggtg gtggatcggc gtcggggagc ggagccatcg
    10621 gttgtcactg taggacaatg tgtcctagag tgcgacacta tgtctcagag atgtccacgc
    10681 catcggatgc gaggcctgac gcagcctcgg gaggcgatga cctcgacggc accgctgatg
    10741 cgaggcaccc catgaccgcg caccccgagt cgtggatccg tgaacgacgc tggtggatcc
    10801 tggcgtgcct gtgcctgtcg ctggtgctcg tggtcgccgg caactcgtcg ctcaacgtgg
    10861 cgctccccaa gatccaggcg gacctggggt cggcccagag cgacctccag tggatggtca
    10921 acgcctacgg cctggtcttc gccgtgctgc tgttgccggc cggcgcgctg gctgaccgct
    10981 acggccggaa gtccgcgctg cagctcggca tggtcatctt cggtgcagcg tccttcgtct
    11041 cgatcttcgc gacggagaac tggcacctca tcgcgctgcg tggcgtcacc ggcctcggag
    11101 cggcgttcat catgccgggg acgctgtcga tcctggcgaa cgtgttccac gatccacgcg
    11161 agcgccaacg cgcgatcgcc atctgggccg gtttcgccgg gctcggaggt gccatcggcc
    11221 cgatcctctc cgggttcctg ctcgagcact actcctgggg atcggtgttc ctgatcagcg
    11281 tcgtcatcgc cgcggtcgcg ttggtggcag gcgcctacct cctgcccaac tcctcggatc
    11341 cccacgaggc gatcctcgat ccgctcggag tgatgctcgc gatcgcagcg gtcgggacgc
    11401 tgctttacgg gatcatcgaa ggccccgaca acggatggac ctcgacgatc accctgggat
    11461 ccatcgctgc tgcagtcgtc ctgatgggcg cattcgtcac gtgggagctg cgtaacccga
    11521 acccgatgct cgatgtccgg ctgttcagga acaggtcgtt ctcggtcggc tctaccacga
    11581 tcactctgca gtacttcgcg gcgttcgggc tgttcttcgc catggcgcag tacctgcaga
    11641 tcgcccacgg ctactcaccg ctccaagccg gcctcgcggg gcttccgatc ggcgtcatga
    11701 gcatgatcgg ggcgccgttg agtgccacgt tcgtgcggcg cttcggccat cggggagtgg
    11761 tcggggtcgg cctgatcatg tcggcggggg gtctcgctct gttgtcgacc gcgtcaccga
    11821 cgacgtcgct ggcgctcctg ctggtcggct tctgcctgct cgggctgggc gcaggtcagt
    11881 cgaccgcgcc gtcgacgacg ctgatcatga ccgcagtccc gcggtccaag gccggtgtgg
    11941 gctcagcggt gaacgacctg tcacgggagc tcggcggagc gctgggcgtc gcggtgctcg
    12001 gcagcgtgat ctcgtcggtg taccgatcca acatcgccgg ccacgtcggc cccgcgctcg
    12061 cctcgaccgc gggaacgtcg gtcaacgaga cgctggccgc agcccagcac gccgcttcga
    12121 gtggctcggg agccgcagcg ggagcgttgg tccacggggc gcagatgagc ttcgcggacg
    12181 ggttcggtgc cgcgatggtg ctcggggcgc tggtcctgct cctgaacgcc gccctggtct
    12241 ggttccgcgg cctcgacgtg gatccgcacg ccggctgggg ctcggacgcg ggcggcaggc
    12301 cacccgtgaa cgccgacgca cccaccgacc cggcgagcgc gtagacccat ctcctctcgg
    12361 ctctccgagg cgagcccgcg agcgctgatc acgctcaggt ggccagcgtg cgttggagcc
    12421 catctcgggc ctcggccacg cacgacgccc atgggcgatc accgagctcg tacgcgccgg
    12481 tgatgccgtc gaatgcgctc gacgccacca cgtcgggctc cagttcggcc agcacgtcga
    12541 gcgtgccgaa catggcgtcg gggtcgccct ggcccagcag gacgacccgc caccgcccgt
    12601 cccggtggaa gatcgtgtcg ccggtactgg tgtgcgatgc cacccaggag ctcgatgtgg
    12661 tcgaggtcgg cgtcggtccc ggtgttgtgg aacatgatgt tgcccgcgtc aggagagacc
    12721 cacagatgcg cgtgggtgac cagccgttcc gattggacgt cgggccgggt ggcccacagg
    12781 tcggcgcgca cctgctgcac ggggctgcct ccgagggtct cgtcaaccgc ggtggacgtg
    12841 gggttgcagc gcccccgggg ggatgacgcc catgcggccg tgctggtagt cgtcgatcgc
    12901 ctgggcgagc tccgcctcgg tgttcatgac gaacggcccg tactggaagg cgggttcgcc
    12961 gatcggttcg ccgcccagca gcatgacctc gagggcgtcg gttcggctgt cctgggtggc
    13021 atcagccgcg agggtcaggc ggtcaccgcg gccgaacacg accatgttgc cgccatggat
    13081 cggcgcctcc tcggtcccgg cggtgccgga cccggcgaac acgtacgcca tcgcgttgaa
    13141 gctcgggttc cacggcagcg acagccgctc gccggcgtgg atcgacgcgt ggatgatggt
    13201 gatcggcgtg tgggtggagc cggggccgac gtgtccgtcc acgtcgcccg cgatcacccg
    13261 gatgatcgcc ccggcgtcgg ggctggtgag cagcaggatg tcccgcttgg tgatgccctg
    13321 gtaggcgggc ggggtccact tggccgacgc agggaggttc acccagatct ggaccccgtt
    13381 gaacagcccg ccggactcga ccatcgaggc cggaggggtc tcgatgtgca ggacgcctga
    13441 tccggcggtc atccactggg tgtctcccgg ttcgatcagg ccgccgccac cgctggagtc
    13501 ctgatgctgg aacaggccgt ccatcaggta ggtcacggtc tcgaagccgc ggtggggatg
    13561 ccagtcggtg cccttcggtt ccccgatccc gtagtcgatc tcaccgaggt ggtccatcat
    13621 gatgaacgga tcggtcagct ggtacggcgc accggccagc gctcgacgga ccgggaaccc
    13681 catcccttcg agaccggtcg ggccggtgga gatgttcgcg accgggcgtt cgacgtcgtc
    13741 gacggccgcg ggggagatgc ggggcagggt gagggtgtcg gcggtgacag cgggcatgtg
    13801 ggctcctccc tacggggtgc ggccggatcg gccggcggcg acctgttcac ggacggcggg
    13861 gacgacccgg tccatgaacg cggacatctg ggcagggtcg tcgccctcgg ctccgaacac
    13921 gaacgcgttc atgccgccgt cgagcacgag accggtcaac gtgtcgatcc actgatcgac
    13981 cgggccgtcg aactcgccgt tggaggggcc gtcggtgatc gtgccccaca cgttgtagat
    14041 ccgcgagatg tccgacgggt cccggccggc gtcggtggcg gcgtcatcga tccgccggtg
    14101 catgtcgggc agcttgtcgg gcgggaagaa cccgctggag ggcagccagc cgtccgcggc
    14161 acgcccgacc agcgccaaca tgcggggccc gccgactccc aaccagatgc ccatgtcgtg
    14221 cgccggttgc ggaccggggt gcaccccggc gatctggtag tgggtgccgg tgaagcggac
    14281 cgagcgttga gcggaccaca tgagacgaac gatctcgacc gcctcggtca acgcctcggc
    14341 ggcttcggcg ccgctgcggt tgggcccgcc catcgccgcg atcgcctccc agaacgcgcc
    14401 ggcgcccaac cccagttcga atcgaccgcc cgagatcaca tcaaggctgg ccgcggcctt
    14461 ggcgagcact gcgggtggcc gcaacggcag gctggcgacg tcggggaaca cgcggatccg
    14521 ctcggtgcgc gccaacacgg tcgccatcag ggcccacgtg tcgaggaact gcttctggta
    14581 cggatggtcc tggatcccga tcagctccag accggcagcg tcagcggcct gagcggtcgc
    14641 caacgtctgg tcgaggatcg ccgtatcggg cgagaggaac agtccgaact ggacatcacg
    14701 gccgtagtca cccatgggtc gcaacctgcc gatcggcctc aggcgtccgc ggagcggatc
    14761 gcggagacgt cgaactcgag ggtcaccttc tcgctgacga gcacgccgcc ggcctccagc
    14821 gcggtgttcc agttgacgcc ccagtccttg cggttgacgg tggtcttgcc ctcgaggccg
    14881 aggcgcgtgt tgccgtaggg gtcgacggcg gtgccggtgt agtcgaactc gacggtcacg
    14941 ggcctggtga cgcccttgat ggtcaggtcg ccggtcacct cgaaggtgtc gtcgccggtc
    15001 ttggcgaccc cggtcgacgc gaaggtgatc tcggggaagg tctccatgtc gaagaagtcg
    15061 ttgctgcgca ggtggccgtc ccggtcggcg ttgccggtgt cgatgctgtt cgcctggatg
    15121 gtcagctgca gctgggagtt ggccgggttc tcctcgtcga aggagccgct gccggtgaac
    15181 tcggtgaagg agccccggac cttggtgacc atggcgtggc gggcgacgaa tccgatccgg
    15241 ctgtgggccg ggtcgatcgt gtaggtgccg gttgcggtgg tggattctgt ggtggacatt
    15301 ctctggctcc tcggggtgaa tggttgatac atcctccaac atagcggatg tgtcaactat
    15361 tcccaaggta tgctcgagga cgtgaccgac gagccggcgt gggtggtctg ggaggccgtc
    15421 cagtcgatgt acgagcaggt ggagaccgag ctgggccgcc gactgggctc cggctcgcag
    15481 ctgtcgtgca ccgactacaa ggtcctggct gcgctcacct cccatcccga cgggcggatg
    15541 cggctgttcg agctggtcga cgcgctcggc tgggagaaga gccgggtgtc ccatcaggtc
    15601 agccgcatga ccgaacgcgg cctgctggac aagacccgct gccccaacga tcgaaggggt
    15661 gcgcacgtgg ggccgaccgc ccgggcccgc gccgcgatgg ccgaggccga accggcctac
    15721 atcgatgcgg tgcaggcgct gctgggtgag acgctcaccc gggctcagct cgcctcgctc
    15781 cacgacgcgg cccgagccgt cctcgcagga atcgccgatc cggacccggt cggcagccag
    15841 cgctgatcgc gaccgctacg gccgcccggg gccgacgagc tcaggctccg acggtgctcg
    15901 agtacacgta gtgggcggcg tcgccgtcga agtcgaacgt ggccgcggac ggcagtagcg
    15961 accccacccg gatcttcacc gagctgggca gcccgttgat cgggatgaag cccgactcga
    16021 gtcccggcag gaacacgcct ccgatcgaga aggcggcgtt gagcaggtcg gtgaacgcac
    16081 cggcggtcgg cagcaggatc tgcacaccca caccgatgac gtcgtcgggg ttgccgagtc
    16141 cctcgatgat ggcggccagg aagctgccga ccgcgttgag gaagttcccg atgttgtcca
    16201 cgatcagcga gatcagttgc tcctgggtgg gcggcaccgg cgacgaggcg atcagcgtct
    16261 cgagcgcgtc gtgcatgacg ggcaccagcg cggattcgaa ggcgtcggtc atggcgcagc
    16321 tgtccaggat gccgtcgcgc tccatgacga aggtcagggt gccgaagatc tcgagcgggg
    16381 cggtgccgag cagcacatcg ccgatgtcga ggttctgggc ctgggggaag gtcacatcgg
    16441 ccgagccggc cgggatcgcc accgtggtgc cgttgggcgc cgcgtcggtg gccggcagcg
    16501 cgcctgagcg gcactgcgag gccacctgga cgtgcgtcga accggcgacg ccgagcttgg
    16561 agcggaaccc gacctggatc acatagggct cgtcgccggc gtccatgtcc tcgcccgtga
    16621 cgaccttgat cgtggcgggc ttgaccttcc agtcggtggg ggccggacct cccaccgtgc
    16681 aggccgctcc gagcagcgac accgcgacga gcaacgcacc gatggccgaa agccgcttca
    16741 tgggaccccc gagagccgtg gccgaccacc cctggtcgga tccgcggagc gtaaccgatc
    16801 ggcgccccgg cgtgtccgat gtctcagcgg tacggaacgg ggcgtcggca aggtgcggct
    16861 cgacggcgcg tctcgaccgt gcgggtggct cagacccgga ccggcgtgta gagcccggtg
    16921 gcgaaggtgc ggtcgtccca gtgtcgcaag gcccacaccg agcccttgga gcacccgctc
    16981 ttgccgggca ccaccatgcc ccgtctctgg gcccgactca ccagctcggg gatcacgtcg
    17041 ccgtggctgc acaggacggc cgtggtggtg acgagctcct cgaccagcgc ccaggactcc
    17101 tcgatcgggg tgccctcggc cagtcgttcg tcgacgacga catcgaggcc cagcgccgcg
    17161 gccagcgggt cgacggtctg cacgcagcgg cggtaggggc tggaacgaac gacgaccggt
    17221 gactcgtgca cgagccagtc gcggatccgc tcggcctgct ggtggccgat cgggtcgagg
    17281 ggccggtcgt cgtcgtgggg atcggagtcg tcgcgcgcgc ccgcggatcc gtgccgcacc
    17341 aggtacagcg tcatcggcgg tgcaccgtca cgtcaggtcc gcggggggtc ggcccatcgc
    17401 cgtcacggta accgcggccc gcggctcggg tggaacccga cgagcgatcg atccggcagt
    17461 ggccgagagc tctgctcaac cgttggatcc gagcgacggg gtgccgtcgt tgcggtcgtt
    17521 gcccccgaag cggatccagt cgccgagggc gtcggcacgg gtcggcaccc gccacaccag
    17581 gaggtggccg tcccggcggg tgatcgccac gtcgccgcgg ccgtcgccgt cccagtcacc
    17641 gaacgccggg gtgcccacgg cccagccgcc agtcagcttg ggccatcccg acggtggcgt
    17701 gccgtcgccg cggaaggcgt cgagcatctg gaccccgttc gctgcgacca cctcgtcgga
    17761 cccgtcgccg tcgacgtccg cgatggcagg ggtgacgaag aaggcgacgt ccgcggtgac
    17821 gcgtgggaag cccggccgga tggctccgtc acggccggac caggcggtca gctgcgggtc
    17881 gccgggctgg tcgttggcga acagggtgtc gagggctcgg cccgcgccgg tggtcggcac
    17941 gaccacgtcc aaccccggac cgctcccgcc gtcggggacg tcggggtcga gacgaccgat
    18001 cgctgctcca ccgaacgcgc tggtcagcgc ggcggtgtcg ctgctgtcgg ttccgggtgg
    18061 caccgccccc agccagttca gcgcgagctg cacgccgaac ggacggccgt aggggctgcg
    18121 gccgtccgcg tcgagcacca tcgtctggcc gctgaccgag gtggcgacca cctcgaccgc
    18181 cccgtcgctg tcgacgtcgc cgatcgcagc ctgggtcgcc accccgtcgc cgatcgtcgg
    18241 cagcaccgcg gtcaggaaca tcggcagcgc gaccggccag cccgccaggt aggcgcggtc
    18301 gtcgggatgg gcggcggacc gatccgcgcc ggcggggtgc ccggtcccgt acgacgagat
    18361 ggcgaacagc cgggtgttgc cgctctggcc gggaagcccg agaccgggga acacggcggg
    18421 gggctcgtcg tactgctcct gggtcccgat cacgatctcg ggccgaccgt cgccgtcgag
    18481 gtcgccgagg gcgggtgtgg ccacgatctc gccgccctga ccggtggcgc cggggctgcg
    18541 gaacgtgacg tggtgcgtgt ccgcgtcgac gtcgtcgacc tgggccgggt cgacgacgag
    18601 caccgggaac ccggccagcg gggagccgtc ggcgtgccag gcgtagacgt gacggtccat
    18661 cgccgcggcc acgacgtcga acgcgccgtc accgtcgagg tcgccgagcg cggcgccgcc
    18721 gacgaagccg cgcttcatcc ggttgcgctc gtcggtcgcc tcgccccggc tgaagcgggg
    18781 gtcgctcgac atcgacgccc ggaccgagcc gtcggcgccg aacacgtgca ccgacccctc
    18841 gagatcggcc acgaccacct cgggcgatcc gtcgccgtca aggtccgcca cggccggggc
    18901 tccgatcccc gaggcacggt ccggtgcgat cacccccgcg gcccgggctg ttgtcgaacc
    18961 ggcgtgccag aacggggcgg tc
//
LOCUS       DAOQOD010000046        18843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1028_length_18843_cov_12.316266, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000046 DAOQOD010000000
VERSION     DAOQOD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_18843_cov_12.316266"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1767)
                     /locus_tag="PLZ72_06475"
     CDS             complement(88..1767)
                     /locus_tag="PLZ72_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA35977.1"
                     /translation="MTSGTADRDQFDLATVNEVVSAAVPDREAVVFGDRRLTYGELAE
                     RSRRLASYLHGKGIGCHTERAELAPHESGQDHVALYLYNGNEYLEGVLGCAKARAVSF
                     NVNYRYVAEELRYLFDNASARGVIYHASFAPVVAELLESMEPEERGRIGVLLQVDDGS
                     GEALLDGAEDYETALAGSDPAGPGLTPSPDDLYIVYTGGTTGMPKGVLWRSDDIFMNA
                     MGGKEVGTWVEMTSYDQIADRARNNAGFRLLALPPLMHGAAQWAAFMMLNSGATIILP
                     ESRTLDAAEVWRLVEDEGVQSIALVGDAMARPLLDELVAGDYDTSSLFVLGNGGAVFS
                     ATLKAEFLEVLPDLLINDSLGSSETGAQASTLSAKGGVRAAEFQPGPGATVVSEDLGS
                     VLEPGHDGIGWSAQSGAVPLGYLGDADKTARTFPTIAGVRYSVPGDRAVWRPDGVIEL
                     LGRDSQCINSGGEKIFVEEVEQAILSHPDVADVLVAGRPSERWGNEVVAVVEMVPGAS
                     PDEADLTAHAGNSVARYKLPKAWVFVEKIHRSPAGKADYRWAKQLAADSAG"
     assembly_gap    1857..1956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2101..2745
                     /locus_tag="PLZ72_06480"
     CDS             2101..2745
                     /locus_tag="PLZ72_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA35978.1"
                     /translation="MTITVFLVDDHEVVRRGVRDLLESTGDVTVVGEAGSVAEAIERV
                     PAAAPDVAVLDVQLPDGTGIELCRELRSQRPELSCLMLTSFPDDDALLDAVVAGASGY
                     VLKQVRGSDLVDAVRQVATGHSLLDPVLRERAAKRIRSGPEEDERLSHLTPQERRILD
                     LLADGMTNRQIADEMFLAEKTVKNYVSNLLAKMGMSRRTEAAVYAARMAERQHR"
     gene            complement(2809..3852)
                     /locus_tag="PLZ72_06485"
     CDS             complement(2809..3852)
                     /locus_tag="PLZ72_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNA35979.1"
                     /translation="MGARSSLLAGHGDDFAWSLFDAAPDAIILVADSGEIAFANEAAG
                     QMFGCPVEDLLRLSVEDLVPAPLRQVHSVRRRDYEEHPRVRQMGSGLELSAVRPDGTE
                     FPVEISLSPMDLSGARFTVAAVRDITERVRAEAAYMESQEALRRAEMRTAVVEDRDRI
                     ARDLHDTVIQRLFGAGLSLQAAASMTEGALRDRLEAVVGDLDEMIFDLRTTIFSLQGT
                     RTLAGGLRGELLDAATEAALASGLDVRVQFDGPIESLDDRVAPHLLPVLREALANVAK
                     HAQATRARVSVVVDADVALVVADDGIGAPDEVFGGSGLSNLAARAEELGGTSSLVPGP
                     DGGSVLEWRVPRT"
     gene            4044..5471
                     /locus_tag="PLZ72_06490"
     CDS             4044..5471
                     /locus_tag="PLZ72_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNA35980.1"
                     /translation="MQPTPSTGEQRSWAKATGFLVALYLVGLALIAGIVLVAGGKDGG
                     GGSAGGSGPGTTIPVSLTEFKIDGQLMAPPGAVTLEVTNDGTMQHDLTIADLGKTTGM
                     IPPGETVTLDLGTLDAGKYGVVCTVAGHEGSGMKATLEVAEGMEGMDHSGSSSSSSQA
                     AAGGQDSTSGEGEMDYAKMDEDMIASFKAFPAKTKGVGNQPLDPTLVLPDGTKQFNLT
                     AKIADWEVEPGKVVKAWTYNGMVPAPRIRLNVGDTAEFVIKNELPVNTDVHWHGITVP
                     FDQDGVAPITQDPIKPGETFTYRFTVNKPEIGMYHPHLHGQMGLPNGMFGEIQVGDTP
                     VVSNRTVSGVQIPADVKPVQDIPMILNDAGVIGFSLNGKSFPATEPIVVKEGEWVTMT
                     YFNEGFQVHPMHLHQFPQLITAVDGIPLDQPYYADTVLVGPGQRFTVQFQATAKGTWV
                     FHCHILNHAEREDGMFGMVTAVVVQ"
     gene            5657..6694
                     /locus_tag="PLZ72_06495"
     CDS             5657..6694
                     /locus_tag="PLZ72_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA35981.1"
                     /translation="MDTVDALTPTWLDRALELSGPRSVTGLEQRPLGTGQMCDSHRIR
                     LTHADGTGSSLIAKFPSSDPSSRSTGLLLRAYEKEIRFYQQLAPTLPVRTPTAHVAAI
                     DTDSGSFVLLLDDLDPARPGDQLAGCDLTAARAALGELVGLHAPRWGDPALADIEWLV
                     GDRGPARDMMIGLLGSMWTGFVDRYRDRLEAHVLEAGAIVFDRLEAFYRDREPTTIIH
                     GDFRLDNLMFHPDGTVTVLDWQTCTVGPGPADAAYFVGAGLLPELRRAHEVELFEHYH
                     RGLVDAGVVVDRDDCWSAYRRGTWAGLVMAVGASMLVERTERGDDMFMAMASRHALQA
                     LDLDAGELLDD"
     gene            complement(6723..8495)
                     /locus_tag="PLZ72_06500"
     CDS             complement(6723..8495)
                     /locus_tag="PLZ72_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNA35982.1"
                     /translation="MALVVALGLLVAIRMLSERSDGSSPTGHRSGSGPDARLVDGIRF
                     TDVTERAGLLEPHSRTDLTGESAMTAGAAVLDLDADGDLDVYLTRIGLPNRMMINDGS
                     GRFTDRAGELGVGGDPYDGSSAAVFGDVDADGDPDLVLTGARRNGTTLLRNDGGHFVD
                     ATAGSGLDDLPPVADGDMAQMHGATFADYDRDGLLDLLVTHWDEVVPAALRDAGDSIT
                     PDAHGRTVCARAQWLAERDFPRADGSPPNRGRLYHNEGGGRFRDVSSALGLPFDEVMG
                     FTGSFVDVDADGWDDLLLTGDFCTSRLFRNDAGTGFTDVTAASGVGTDENGMGSVVTD
                     LDGDGHPDWFVTGIGPVGDAAAPPQLGGFGSSGNRAYLNDGDGTFTDATDRLGLRNGG
                     WGWGAAVEDFGNDGRREVVMTNGYGIGEASDPDRSSGTPADDPLVFWVPDGATFVPAA
                     ADVGLDDTGLGRALVPFDMDRDGDLDLLIANFGTPPRLYRNDTPTRHWVVVRLDDQTT
                     PGNRGAIGARITVTPTDGRPVTGWITGGGSYESQLPPEVHIGLGPKKSIERVEVLWPG
                     DPTPQVLDGVAGDRVLVVERATGS"
     gene            8627..10138
                     /locus_tag="PLZ72_06505"
     CDS             8627..10138
                     /locus_tag="PLZ72_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35983.1"
                     /translation="MTVPTVMVGEREEGHPDPSVADGDVHRWRFQPPRLRDPRLHVAA
                     VVVSVQVLGQVALGFDLSIAQILVCLLTAAAIELALTAPRTRVVAWPASALLTGNGIA
                     LILRVPGTEHGDWWSMRGWYVFAATTAVGVLSKYVLRSRGRPLFNPSNLALVVTFLVL
                     GSGIADPQDLWWGPMSVGLAATYAIVVVGGVLVTRRLDLLRVSVAFWSVFAALMGVLA
                     ISGHAMTARWNLGPVAGMQFWTTLALSPEVLIFVFFMITDPRTGVTGRAGGVLYGAAV
                     ALTSGVLIAFQSTEYATKVALLAGLVVVCALRPVLEHLAPADPPVGVLRWTRQRRGRA
                     MSVAAIGIVAVGLTVLGSSTTGSVPEPRIGDRPAVELGEDQRPRVEIGDGLDGLGGSF
                     GTERARQVADDLVRALLLADRALADGDDDLADEVATGPFRRDLSDRAAAPAPSRTVAR
                     ALVDVVRDPDDFQAQPRLAITLTGTVDGSEWSATYHVLATTADVRIERELRPG"
     gene            complement(10336..11784)
                     /locus_tag="PLZ72_06510"
     CDS             complement(10336..11784)
                     /locus_tag="PLZ72_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA35984.1"
                     /translation="MATPVIVGTAQSIDRPGDHDLLDAPGPIELMVAAARAAADDAGA
                     PTLLDRVGWIGVTGGFYRYRNPAAVVAEELGLSGVGTAITEITGTAPQDMVGLACERI
                     AAGELDAALVLGGEARWTNQQLKRQGLSPRWNESPGEGTPELIGGFAPEAISEMAQLG
                     SATVFYALFEDALRLSHGRTVDEQRDHCAELWARESRVAEANPYAWDRTRHEVAAIRD
                     ESEQNRMICFPYPKAMVANNTVNMASAVLICSTELARSAGVADDRRVFPHVVTSSHET
                     WEVRRRAELTGCPALTAAGEAALEHSGLDVSDIEHVDLYACFPSIVQMSSAALGLDEG
                     RPLTVTGGLGFAGAAISNSVGHSIAAMVPLVRAGGAGLVHGNGGSATKHSFAVYSPEP
                     PRAFARIDCQDRVDLHEREPLDESFDGPVTVDAASVRFDREGPTDVLAAVRSEDGARA
                     WATSADPGILDEAMRNGISGREGRSAAGSLHL"
     gene            12018..13436
                     /locus_tag="PLZ72_06515"
     CDS             12018..13436
                     /locus_tag="PLZ72_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNA35985.1"
                     /translation="MLLASLGAACGGSSDDATGRGSTDPGTDTATCATDLADGPTEIT
                     VWHPYNTLTKTTLESIAEAYNASQSKVHVNVEAQGNTIELQKKFDEMMATPDQLPDVV
                     FTEDTNLQFMSDSGVVVPASACQSSDQSGGRYLDGLFPAVTAAYTVQGTLWPAAFSVS
                     TLMMYANNAQLRAAGLEPGVFPETLDELRTMSEKIKAANLPGVKAPLVMKLDPWVLET
                     LLTGSGQLIVNHGNGRDELASASELDNRSSVEVLEWIQGMVDDGLLTTIPYGDSIDDF
                     LAFSQGNSSFIISGQRSITTVAAVMDGQTQGVDIAGAPSSDVLQKLDIEAGLFPGLEE
                     AGRSGVSGSAGYVVGGSDARTAAAWDFMKFFNALPQQQRWVLEGSYVPSSQAVLDSPE
                     VQKAFTTTRGGRWTATVSEGIAELDPDFPGPLVGPYGAYRDRLNRLLDEVGAGAEVRP
                     ALTAASDDITDLLRSYRSEVGG"
     gene            complement(13489..14457)
                     /locus_tag="PLZ72_06520"
     CDS             complement(13489..14457)
                     /locus_tag="PLZ72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNA35986.1"
                     /translation="MTVPEPSSAAPGSEPFVAEAEVLGRTLPPTVRSALLRMVDSPPR
                     EGAILVRGVPVGELPPTPATPTEPTTKDHVSELVLTAVARTLGEPVGYRPEHGGDLVQ
                     NLVPTVAGADRQVSTSSRVDLAFHTETAFHPHAPRFLVLLCLRGHPDARTTLCSVHDL
                     IDALDGDTLSVLRQARFVCGVDESFLDGARAALASTERRSGPGSVSEASTGPLGPRGP
                     FPVIGGTADRPTLWYDADLMRGTDPAAQAALDRLDRAVAEHHCSVVLEAGDCLVVDNT
                     AAVHGRSSYQARFDGTDRWLQRAFVVTDLDESAADRDGRIIQTLFA"
     gene            complement(14551..15417)
                     /locus_tag="PLZ72_06525"
     CDS             complement(14551..15417)
                     /locus_tag="PLZ72_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNA35987.1"
                     /translation="MTEPADTGAISLDDRLADASGSLSAAERRVAEVVVADRELVAFG
                     TVARIAERAGTSGATVVRFADRLGFSGFRGLQESVRADLSHRLLPAAARIRRPVSDDL
                     VGGLGRSVAASLERTLRHLDPAEVDRAVRLLAGRRRRVFVMGGDAGSGVAHQVSVHLS
                     MLREGVEELVGGAVEVGRTIARSGPDDVLVVIDLPRYDRAVLDVVAWARGRGLRTIAV
                     TDSSLSPVAAGADGVLTVQSHDVGPFDSYVSALAVGEVLVAGVAVRMGRSAVDRLDAL
                     ESVWVDTDALSD"
     gene            15507..16835
                     /locus_tag="PLZ72_06530"
     CDS             15507..16835
                     /locus_tag="PLZ72_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNA35988.1"
                     /translation="MAASIGSLMEEPDPGHDGRSVLLLDRTGGPMTEAWIIDACRTPR
                     GTGKVGKGSLAHLHTQRLGATVLAALAERTGLDTGDVDDVIFGCSWQVGTQSGDLGRM
                     AALDAGYPITASGVTLDRFCGSGITAVNLAAASVMSGLEDVVVAGGAEMMSSYKQNAP
                     KNRNPFMDNGNLHLRAQHPQTNQGVAADAIATLEGIPRSALDALAAESQRRAEVAISE
                     GRFERSLVPVLDEDGTVALDREEYPRPGTTVDSLAQLPASFEALADMPLDEDGTTYRA
                     MVLARYPGLEIEHVHHAGNSSGVVDGAAAILVTSPEYARAHGLTPRARVRAIANMGDD
                     PTLMLNAPVPATRKVLERAGMTLDDIDLFEVNEAFAVVPEKYIRDLDIDREKINVNGG
                     AIALGHPIGATGSLLIGTVLDELERRDLNVGLVTMCAAGGMAPAVVIERI"
     gene            16871..>18843
                     /locus_tag="PLZ72_06535"
     CDS             16871..>18843
                     /locus_tag="PLZ72_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNA35989.1"
                     /translation="MSIAINEDHVALADTVAAFLTQRGSLTEARARLDGAPEALPDFW
                     GELAGLGWLGLHLGEDVGGSGFGLEELVVVAEQTGRALMPGPFLPTVIASAAVQSAGT
                     DEQRARLLPGLADGSVTAAVALDGDVTVSDGKASGFVRTAVGAGIADLLLVPAGSDVV
                     VVTLRDSGPAADPVAGVSIEVPDNLDPSRRSGRVTLDGAPAETVAGGAGALVDSARLL
                     FSAEAVGIARECTEQAAEYAKERQQFGRPIAMFQAVKHHCANMLVATELATGNVWDAA
                     RTEAGTDAFSAAAAAAATLAIPAADLCAQLNIQVHGGIGFTWEHDAHMFLRRATAVER
                     VVSADHAASALTDLHRSGVRRSVTIELPPEAEAFRDEVRTFAERIKGLPADEQRTELI
                     DSGYVMPHWPAPWGRDAGAVEQLVIEQEFSAAGIQKPGYGITGWVILTLIQYATEDQI
                     ARWVRPALEQEVIWCQLFSEPDAGSDAAGITTRATRVDGGWLLNGQKVWTSGAHVAGF
                     GFATVRTDPDVPKHDGITVMVIDMHAEGVEVRPLKMPMGSSEFNEVFFTDVFIPDDDV
                     VGPVDGGWTVARATLGNESVSIGGGQGGMAMPAEMLIDAYDANPTSLAGGSERVGRYA
                     ANNRACDILNLRSAHRAVAGAAPGPEGAITKLVL"
BASE COUNT         2776 a         6977 c         6435 g         2555 t
ORIGIN      
        1 ccccgccgtc gtcagctcag cccgcggagt ccgcggcgag ctgcttggcc cagcgcgccg
       61 cagcccgccc gccccgccgt cgtcagctca gcccgcggag tccgcggcga gctgcttggc
      121 ccagcgatag tccgccttgc ccgccgggga gcggtggatc ttctcgacga acacccacgc
      181 cttcggcagc ttgtagcggg ccaccgagtt gcccgcgtga gcggtcaggt cggcttcgtc
      241 gggcgacgcg ccgggcacca tctcgaccac ggcgacgacc tcgttgcccc accgctcgct
      301 cggccgcccg gcgacgagga cgtcggccac gtcggggtgc gacaggatcg cctgctcgac
      361 ctcctcgacg aagatcttct ctccccccga gttgatgcac tgcgaatcgc ggccgagcag
      421 ctcgatgacc ccgtcggggc gccacaccgc ccggtcgccc gggaccgagt agcgcacgcc
      481 ggcgatggtc gggaacgtgc gggcggtctt gtcggcgtcc ccgaggtagc cgagcggcac
      541 cgccccgctc tgcgccgacc agccgatgcc gtcgtggccg ggttcgagca ccgatcccag
      601 gtcctcgctc accaccgtcg cgcccggtcc gggttggaac tcggcggccc gcacgccgcc
      661 cttggccgac agggtggacg cctgggcgcc ggtctccgag gaccccagcg agtcgttgat
      721 cagcaggtcg ggcagcacct cgaggaactc ggccttgagc gtggcgctga acaccgcccc
      781 gccgttgccg agcacgaaca acgacgacgt gtcgtagtcg ccggcgacga gctcgtccag
      841 cagcgggcgc gccatggcgt cgccgaccag ggcgatcgac tggaccccct cgtcctcgac
      901 cagacgccag acctcggccg cgtcgagcgt ccgcgactcc ggcaggatga tcgtggcgcc
      961 cgagttcaac atcatgaacg ccgcccactg cgcagccccg tgcatgagcg gcggcagcgc
     1021 cagcagccgg aacccggcgt tgttgcgggc ccgatcggcg atctggtcgt acgaggtcat
     1081 ctcgacccag gtgccgacct ccttgccgcc catggcgttc atgaagatgt cgtcggagcg
     1141 ccacagcacg cccttgggca tcccggtcgt cccgccggtg tagacgatgt acaggtcgtc
     1201 gggcgacggg gtgagccccg gaccggccgg gtccgacccc gcgagcgcgg tctcgtagtc
     1261 ctcggctccg tccaggagcg cctcgccgga gccgtcgtcg acctggagca gcaccccgat
     1321 tcgaccgcgc tcctcgggct ccatcgactc gagcagctcg gcgacgacgg gagcgaagct
     1381 ggcgtggtag atcacgccgc gggccgaagc gttgtcgaac aggtagcgga gctcctcggc
     1441 gacgtagcgg tagttcacgt tgaacgacac ggcccgggcc ttggcacagc cgaggacgcc
     1501 ctccaggtac tcgttgccgt tgtagaggta cagcgccacg tggtcctgac ccgactcgtg
     1561 gggagccagc tcggcgcgtt cggtgtggca gccgatcccc ttgccgtgca ggtaagaggc
     1621 cagacggcgg ctccgctcgg ccagctcgcc gtaggtcagg cgacggtcgc cgaagacgac
     1681 cgcctcgcgg tcggggaccg cggcggacac cacctcgttc accgtcgcga ggtcgaactg
     1741 gtctcgatct gctgtgccgg acgtcatggt ccagacagta gcgagggatc accgccgcca
     1801 tctgacgggc cgtcagctcg actccctcgt cccgtcggga ccctcggctc ccccgtnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg accttcggct cccccgtcgg
     1981 gaccttcggc tcccccgtcg ggaccttcgg ctcccccgtc gggaccttcg gccctgtgca
     2041 cccgcggccc ggacacggca gccttgaccc acggtccacg acccgacagg aaatgagcca
     2101 atgaccatca ccgtgttcct cgtggacgac cacgaggtcg tccgacgagg cgtgcgcgac
     2161 ctcctcgaga gcacgggtga cgtgaccgtc gtcggagaag ccggctcggt ggccgaggcc
     2221 atcgagcggg tccccgccgc cgcccccgac gtggccgtgc tcgacgtgca gctgcccgac
     2281 gggaccggga tcgaactgtg ccgggagctg cggtcccagc ggccggagct gagctgcctc
     2341 atgctcacct cgttccccga cgacgacgcc ctcctcgatg cggtggtggc cggcgcctcg
     2401 ggctacgtgc tcaagcaggt ccgcggctcg gacctcgtcg acgcggtccg acaggtggcc
     2461 accggacact cgctgctcga ccccgtgctt cgtgagcggg ccgccaagcg gatccgcagc
     2521 gggcccgagg aggacgagcg cctcagccac ctgacccccc aggagcgacg catcctggac
     2581 ctgttggccg acggcatgac caaccgccag atcgccgatg agatgttcct ggccgagaag
     2641 accgtcaaga actacgtgtc gaacctgttg gccaagatgg gcatgagccg ccgcaccgaa
     2701 gcggccgtgt acgcggcccg gatggccgaa cgccagcacc gctgaggtcc tggagcgggc
     2761 cgatcggccc cgcggtcgcg gtcgccgact tcgacccgac cggacccgtc aggtccgcgg
     2821 cacccgccac tcgaggaccg atcccccatc ggggccgggc accagcgacg acgtcccccc
     2881 cagctcctcg gcccgggcgg ccaggttcga caggcccgaa ccgccgaaca cctcgtcggg
     2941 ggcaccgatg ccgtcgtcgg cgacgaccaa cgccacgtcc gcgtcgacca ccaccgacac
     3001 gcgggcccgg gtggcctggg cgtgcttggc cacgttggcc aacgcctcgc gcagcaccgg
     3061 cagcagatgc ggagctacgc ggtcgtcgag cgactcgatc ggcccgtcga actgcacccg
     3121 gacgtccagc ccggacgcca gagcagcctc ggtggcggcg tcgagcagct cgccgcgcag
     3181 accgccggcc aacgtccggg tgccctggag cgagaagatc gtcgtccgca ggtcgaagat
     3241 catctcgtcg aggtcgccca cgacggcctc gagccggtcc cgcagagctc cctccgtcat
     3301 cgacgcggcc gcctggaggc tgagccccgc accgaacagg cgttggatca ccgtgtcgtg
     3361 caggtcgcgg gcgatgcggt cccggtcctc gaccaccgcg gtgcgcatct cggcgcgccg
     3421 cagcgcctcc tggctctcca tgtaggccgc ctcggcccgc acccgttcgg tgatgtcgcg
     3481 cactgcggcg acggtgaagc gtgcgccgga cagatccatg ggactcaggc tgatctccac
     3541 cgggaactcg gtgccgtcgg gacggaccgc agagagctcc agccccgagc ccatctgccg
     3601 gacacggggg tgttcctcgt agtcgcgccg tctgaccgag tggacctgcc gcagcggagc
     3661 cggcaccagg tcctcgacgc tcaggcgcag caggtcctcg acaggacagc cgaacatctg
     3721 gcccgcggcc tcgttggcga acgcgatctc accggagtcg gccaccagga tgatcgcgtc
     3781 cggggcagcg tcgaacagag accatgcgaa gtcgtcgccg tgaccggcga gcagcgagct
     3841 gcgagctccc acggctcagc gccgtcctga tccgacgtcg gtgtgcgcac ccaccgtgca
     3901 ggaccgtacc ggacccggga ccgccaggag cagccggcag cgacccccga cccaccgatt
     3961 cgggaccttg gtccctttgc gccgtggccg ccgacggcac aggatgcatg acgttcccga
     4021 agtccgtccc gagaggatca cgaatgcaac caaccccctc caccggcgag cagaggtcct
     4081 gggccaaggc cacagggttc ctcgtcgcgc tctacctggt cggactggcc ctgatcgccg
     4141 ggatcgtcct ggtcgccggc ggcaaggacg gcggcggggg ctccgccggc ggctccggcc
     4201 ccgggacgac catccccgtg tcgctgaccg agttcaagat cgacggccag ctcatggccc
     4261 cgcccggcgc agtcaccctc gaggtgacca acgacggcac catgcagcac gacctgacca
     4321 tcgcggatct cggcaagacg accgggatga tcccgcccgg cgagaccgtc accctcgacc
     4381 tcggcaccct cgacgccggc aagtacggcg tcgtctgcac cgtcgcgggc cacgagggat
     4441 ccggcatgaa ggccaccctc gaggtggccg agggcatgga gggcatggac cactccggtt
     4501 cgtcgagctc ctccagccag gccgccgccg gcgggcagga cagcacctcg ggtgagggcg
     4561 agatggacta cgccaagatg gacgaggaca tgatcgccag cttcaaggcg ttcccggcca
     4621 agaccaaggg cgtcggcaac cagcccctcg acccgaccct ggtcctcccc gacggcacca
     4681 agcagttcaa cctgaccgcc aagatcgccg actgggaggt cgagccgggc aaggtcgtca
     4741 aggcctggac ctacaacggc atggtccccg cccctcggat ccggctgaac gtcggcgaca
     4801 ccgccgagtt cgtcatcaag aacgagctcc cggtcaacac cgacgtccac tggcacggca
     4861 tcaccgtccc cttcgaccag gacggcgtcg ccccgatcac ccaggatccg atcaagccgg
     4921 gcgagacctt cacgtaccgc ttcacggtca acaagcccga gatcggcatg taccacccgc
     4981 acctgcacgg ccagatgggt ctgcccaacg gcatgttcgg cgagatccag gtcggcgaca
     5041 cgccggtcgt gagcaaccgg accgtcagcg gcgtgcagat cccggcggac gtcaagcccg
     5101 tccaggacat cccgatgatc ctcaacgacg ccggcgtcat cggcttctcg ctgaacggca
     5161 agtccttccc cgccaccgag ccgatcgtgg tcaaggaggg cgagtgggtc accatgacct
     5221 acttcaacga gggcttccag gttcacccga tgcacctgca ccagttcccg cagctgatca
     5281 ccgcggtcga cggcatcccg ctcgaccagc cctactacgc cgacacggtg ttggtcggtc
     5341 ccggacagcg cttcaccgtg cagttccagg ccaccgccaa gggcacctgg gtgttccact
     5401 gccacatcct caaccacgcc gagcgcgagg acgggatgtt cggaatggtg accgccgtcg
     5461 tcgtccagta gcgcagcaga tcgcgacccg ggtccgagcc accgctcccc cgatcgcacc
     5521 gacctccgga cggggaggca ggaccccggg gacctcacgg tccccggggt ccgacgcgtc
     5581 cggctccgac gcccggcgct actgtcggcg gacgcgggcg gactcgcagt gggggtggag
     5641 gttgaccgtc gagatcgtgg acaccgtcga cgcgctgacg ccgacgtggc tcgaccgggc
     5701 cctggaactg tccgggccgc gttcggtcac cggcctggaa caacgcccgc tcggcaccgg
     5761 tcagatgtgc gacagccacc ggatccgact gacccacgcc gacgggaccg gttcgtcgct
     5821 gatcgccaag ttcccgtcga gcgatccgtc gagccgcagc accgggctgc tcctgcgggc
     5881 ctacgagaag gagatccggt tctaccagca gctcgctccg acgctgccgg tgaggacgcc
     5941 gaccgcgcac gtcgccgcca tcgacacgga ttccgggtcg ttcgtgctgc tcctcgacga
     6001 cctcgacccg gcccggcccg gcgaccagct cgccggctgc gacctcaccg cggcccgtgc
     6061 cgcactcggc gagctggtcg gcctgcacgc cccccgctgg ggcgaccccg ccctggccga
     6121 catcgagtgg ctcgtcggtg accggggccc ggcccgcgac atgatgatcg ggctgctcgg
     6181 ctccatgtgg acggggttcg tcgaccgata ccgcgaccgc ctggaggccc acgtgctcga
     6241 ggccggagcg atcgtgttcg accggctgga ggcgttctac cgggaccgcg aaccgacgac
     6301 gatcatccac ggcgacttcc gtctggacaa cctgatgttc caccccgacg ggaccgtcac
     6361 cgtcctcgac tggcagacct gcaccgtcgg tcccggcccc gcggatgccg cctacttcgt
     6421 gggagccggg ctcctgcccg agctgcgcag ggcccacgag gtggagctgt tcgagcacta
     6481 ccaccggggt ctcgtcgacg ccggcgtggt cgtcgaccga gacgactgct ggagcgccta
     6541 ccgccggggc acctgggccg ggctggtcat ggcggtgggg gcgtcgatgc tcgtcgagcg
     6601 caccgagcgc ggtgacgaca tgttcatggc gatggcgtca cggcacgccc tgcaggccct
     6661 cgacctcgac gccggcgagc tactcgacga ctgagcacgg gtccgaccgc cagtcgcgac
     6721 cttcaggacc ccgtcgcccg ttccaccacg aggacccggt cgccggccac tccgtcgagc
     6781 acctggggtg tcgggtcgcc gggccagagc acctcgaccc gttcgatcga cttcttcgga
     6841 cccagaccga tgtgcacctc cggtggcagc tgggactcgt aggacccacc gccggtgatc
     6901 cagcccgtca ccggtcgtcc atctgtcggg gtgaccgtga tgcgggcacc gatggcccca
     6961 cggttccccg gcgtcgtctg atcgtcgagt cggaccacga cccagtgtcg ggtcggggtg
     7021 tcgttgcggt acagccgcgg cggcgtgccg aagttggcga tcagcaggtc gaggtcgccg
     7081 tcgcggtcca tgtcgaaggg caccagagcc cgccccagcc cggtgtcgtc caggccgaca
     7141 tcggctgcgg ccgggacgaa cgtcgcgccg tccgggaccc agaacacgag gggatcatcg
     7201 gcaggggttc ccgacgaccg atcgggatcg gacgcctccc cgatgccgta gccgttggtc
     7261 atcaccacct cacgacggcc gtcgttgccg aagtcctcga ccgcggcgcc ccacccccat
     7321 ccaccgttgc ggagtcccaa cctgtccgtg gcgtcggtga aggtcccgtc accgtcgttg
     7381 agataggccc ggttgcccga ggaaccgaac cctccgagct gcggaggggc tgccgcgtct
     7441 ccgaccgggc cgatgccggt gacgaaccag tcggggtggc cgtcgccgtc caggtctgtg
     7501 accaccgacc ccatgccgtt ctcgtcggtg cccacgccag aggccgcggt cacgtcggtg
     7561 aagccggtgc cggcgtcgtt gcggaacagc cggctggtgc agaagtcgcc ggtgaggagc
     7621 agatcgtccc agccgtcggc gtcgacgtcg acgaagcttc ccgtgaagcc catgacctcg
     7681 tcgaacggaa gcccgagcgc ggacgagacg tcccggaacc ggccgccgcc ctcgttgtgg
     7741 tagagccggc cgcggttggg gggcgagccg tcggcacgcg ggaagtcgcg ctcggccaac
     7801 cactgcgccc gggcgcacac cgtcctcccg tgcgcgtcgg gggtgatcga gtcgccagcg
     7861 tccctcagcg cggcggggac gacttcgtcc cagtgcgtga cgaggaggtc gagcagcccg
     7921 tcacggtcgt agtcggcgaa ggtcgctccg tgcatctgcg ccatgtcacc atcggcgacc
     7981 ggaggcaggt cgtcgagccc gctgccggca gttgcgtcca cgaagtggcc tccgtcgttg
     8041 cggagcagag tggtgccgtt gcggcgggct ccggtgagga ccaggtcggg atcgccgtcg
     8101 gcatccacgt ctccgaacac ggccgctgac gacccgtcgt aggggtcgcc tccgacaccg
     8161 agctcgccgg cgcggtccgt gaaccgcccg gagccgtcgt tgatcatcat ccggttcggc
     8221 aacccgatgc gggtcaggta cacgtccagg tcgccgtcgg cgtcgagatc gaggacggcc
     8281 gcgccggcgg tcatggccga ctcgccggtc aggtcggtgc ggctgtgcgg ctcgagcagt
     8341 cccgccctct cagtgacgtc ggtgaagcgg atgccgtcga cgagccgagc gtcgggcccc
     8401 gacccggatc ggtgacccgt cggcgaagaa ccgtcggacc gttccgacag catccgaatc
     8461 gccaccagga gaccgagggc gacgacgagc gccacgaccg gcaccgcccg ccgcgctgat
     8521 ggcgaccgcc gctgaaccga gtcacccccc acgagagctc agcgtaccgg cgcacccttg
     8581 ccggtaccgg cggtcgtcgt catactcgcg gggccgctca ggggaggtga ccgtgccgac
     8641 ggtgatggtg ggggaacggg aggaaggcca ccccgacccg tccgtggcgg acggcgacgt
     8701 gcaccggtgg cggttccagc ccccgaggtt gcgtgacccg cgtctgcacg tcgccgcggt
     8761 cgtggtgtcg gtccaggtgc tcggccaggt cgcactcgga ttcgacctgt cgatcgcaca
     8821 gatcctggta tgtctgctga ccgccgcggc gatcgagctg gcgctgacgg ccccacgcac
     8881 ccgggtcgtg gcgtggccgg cgagcgcgct gctgaccggc aacggcatcg cgctgatcct
     8941 gcgtgtcccc ggcaccgagc acggcgactg gtggtcgatg cggggctggt acgtgttcgc
     9001 ggccaccacg gcggtcggcg tgctgtcgaa gtacgtcctc cgatcgcggg ggcggccgct
     9061 cttcaacccg tccaacctgg cgttggtggt gacgttcctc gttctgggtt ccgggatcgc
     9121 cgacccgcag gacctctggt ggggcccgat gtcggtgggc ctcgccgcca cgtatgcgat
     9181 cgtggtcgtc gggggcgtgc tcgtcacccg gcggctcgac ctgctccggg tgtcggtcgc
     9241 gttctggtcg gtgttcgcgg ccctcatggg cgtgctggcg atcagcggcc acgccatgac
     9301 cgcccgatgg aacctcggtc cggtggccgg aatgcagttc tggaccacgt tggccctatc
     9361 gcccgaggtg ctcatcttcg tgttcttcat gatcaccgat cctcgaaccg gcgtgaccgg
     9421 gcgcgccgga ggtgtcctgt acggcgccgc ggtggcgctc accagcggag tcctgatcgc
     9481 gttccagagc accgagtacg cgaccaaggt cgcgttgctc gccggcctgg tcgtcgtctg
     9541 cgccctgcgt cccgtcctcg agcacctggc gccggccgac ccgcctgtcg gtgtgctcag
     9601 gtggacccgg cagcggaggg gccgcgccat gtcggtggct gcgatcggga tcgtcgccgt
     9661 cggcctgacg gtgctgggtt catcgaccac cggatcggtt cccgagccgc ggatcgggga
     9721 ccgtcccgcc gtcgagctcg gagaggacca gcggccgcgg gtggagatcg gcgacgggct
     9781 cgatgggctc ggcggctcct tcgggaccga gcgggcccga caggtcgccg acgacctggt
     9841 cagggcactg ctgctggccg accgtgccct ggccgatggc gacgacgacc tggccgacga
     9901 ggtggcgacc ggccccttcc gacgggacct ctccgaccga gccgccgccc cggcgccgtc
     9961 gcggacggtc gcccgagcgc tcgtcgacgt ggtgcgcgac cccgacgact tccaggccca
    10021 gcctcgcctg gcgatcaccc tcaccggcac cgtcgatgga tccgagtggt cggcgaccta
    10081 ccacgtcctc gcgaccacag cggacgtgcg catcgaacgc gagctgcgac cgggctgacc
    10141 gccgccggcg caacgcgctc cgctgccggc ggaggcagac gggtccgcgg cctcagtccg
    10201 tccggggaga tgtcgcggcg tagccctgct ccacgctcat gacccccatc gccgcgtggt
    10261 cctcgcggcg cggcgacgca tcggtgtcgc tcgccccggc cggctgggag agacgctgtc
    10321 cggagccagg cgccctcaga ggtgcagtga cccggccgcc gagcgcccct ctcggcccga
    10381 gatgccgttt ctcatcgcct cgtcgaggat gcccggatcc gcgctggtcg cccaggctcg
    10441 ggcgccgtcc tcggagcgca ccgcggccag cacgtcggtc gggccctcgc ggtcgaagcg
    10501 caccgacgcg gcgtcgaccg tcacgggacc gtcgaagctc tcgtccagcg gttcccgctc
    10561 gtgcaggtcg acccgatcct ggcagtcgat gcgggcgaac gcccgcggcg gctccggcga
    10621 gtacaccgcg aagctgtgct tggtggcgct gcccccgttg ccgtggacga gccccgctcc
    10681 cccggcccgc accagcggaa ccatcgccgc gatcgagtga ccgacagagt tgctgatcgc
    10741 ggcaccggcg aaccccagcc cgccggtgac ggtcagggga cgaccctcgt cgagcccgag
    10801 agcggccgac gacatctgca cgatcgacgg gaagcaggcg tacaagtcga cgtgctcgat
    10861 gtcggacacg tccagcccgg agtgctcgag cgccgcctcc cccgccgcgg tgagcgccgg
    10921 gcacccggtc agctcggcgc gtcggcgcac ctcccaggtc tcgtgcgagg aggtgacgac
    10981 gtgcgggaac acccgccggt cgtcggccac gccggccgag cgggccagct cggtcgagca
    11041 gatcaggacc gccgacgcca tgttgaccgt gttgttggcc accatcgcct tggggtacgg
    11101 gaagcagatc atccggttct gctccgattc gtcgcggatg gctgcgacct cgtggcgggt
    11161 gcggtcccag gcgtacgggt tcgcctccgc cacccgcgac tcccgcgccc acagctcggc
    11221 gcagtggtcg cgttgttcgt ccaccgtgcg gccgtggctc agccgcagcg catcctcgaa
    11281 cagcgcgtag aacaccgtcg ccgatccgag ctgcgccatc tcggagatcg cctcgggggc
    11341 gaaccctccg atcagctccg gcgttccctc gcccggcgac tcgttccagc gcggcgacag
    11401 gccctggcgc ttgagctgct ggttggtcca acgggcctcg ccgcccagca ccagcgccgc
    11461 gtcgagctct ccggccgcga tccgctcaca tgcgaggccg accatgtcct ggggggcggt
    11521 tccggtgatc tcggtgatgg cggtgccgac accactgagt ccgagctcct cggccacgac
    11581 cgcggccggg ttccggtagc ggtagaagcc tccggtgacc ccgatccagc cgacccgatc
    11641 gagcagggtg ggcgcccccg cgtcgtcggc cgcggcccgg gcggccgcga ccatgagctc
    11701 gatgggcccg ggcgcgtcga ggaggtcgtg gtcgcccggg cggtcgatgc tctgggccgt
    11761 ccccacgatg acgggggtgg ccacgggacg caggggcgcg ggaccggggt cgttctgtgt
    11821 cggcacgggc gacagtctgg cagcgccgtg acgctgtgat cgccggcacg caccgcgaac
    11881 gacgtcgttc ccggcagact gtgacacagg ggggatcgag acccggaagg gatcgcgtgg
    11941 tggagaaggc agtgccgatg tcccgaacgt cacgacgccg cagtcgcggg caccggtggg
    12001 tgcgctgcgg tgccgtggtg ctcctcgcgt cgctcggtgc cgcctgcggc gggagcagcg
    12061 acgacgcgac gggccgaggc tcgaccgatc ccgggaccga caccgccacg tgcgccacgg
    12121 acctggccga cgggccgacc gagatcaccg tgtggcaccc ctacaacacc ctgaccaaga
    12181 ccacgctcga gtcgatcgcc gaggcctaca acgcgtcgca gtccaaggtg cacgtgaacg
    12241 tcgaggccca gggcaacacc atcgagctcc agaagaagtt cgacgagatg atggccacgc
    12301 ccgaccagct ccccgacgtg gtgttcaccg aggacaccaa cctccagttc atgagcgact
    12361 ccggcgtcgt cgtgccggct tcggcgtgcc agagctccga ccagagcggc ggtcggtacc
    12421 tcgacgggct cttccccgcc gtcaccgccg cgtacacggt gcagggcacc ctgtggcccg
    12481 ccgcgttcag cgtctcgacg ttgatgatgt acgccaacaa cgcccagctc cgcgccgccg
    12541 gcctcgagcc gggtgtcttc cccgaaacgc tcgacgagct gcggaccatg tccgagaaga
    12601 tcaaggccgc gaacctcccc ggggtgaagg cgccgctggt gatgaagctc gacccgtggg
    12661 tgctcgagac gctgctcacc gggtcggggc agctcatcgt caaccacggc aacggccgcg
    12721 acgagctcgc ctcggcctcc gagctcgaca accgcagctc cgtcgaggtc ctggagtgga
    12781 tccagggcat ggtcgacgac gggctgctca ccaccatccc ctacggcgac agcatcgacg
    12841 acttcctggc gttcagccag ggcaactcca gcttcatcat cagcggccag cggtcgatca
    12901 cgactgtcgc cgcggtgatg gacggccaga cccagggcgt cgacatcgcc ggcgccccga
    12961 gctcggacgt ccttcagaag ctcgacatcg aagccgggtt gttccccggc ctggaggagg
    13021 ccggccgctc cggggtgtcg ggcagcgccg gctacgtggt cggcgggtcc gacgcccgga
    13081 ccgcggccgc gtgggacttc atgaagttct tcaacgccct cccccagcag cagcgttggg
    13141 tgctcgaggg gtcctacgtg ccgtcgagcc aagcagtgct cgactcgccc gaggtgcaga
    13201 aggcgttcac cacgacccgg ggtggacgct ggaccgccac cgtctccgag gggatcgccg
    13261 agctcgaccc ggacttcccg ggtccgctcg tcggcccgta cggcgcgtac cgggaccggc
    13321 tcaaccgact gctcgacgag gtcggcgcag gcgccgaggt ccgaccggcg ctgaccgcgg
    13381 catccgacga catcaccgac ctcctgcgca gctaccgctc cgaggtcggc ggctgagctg
    13441 cccgccgagc cggatccggc cggcggatgg gctggcggcg agcgggcgtc aggcgaacag
    13501 cgtctggatg atccggccgt cccggtcggc ggccgactcg tcgaggtccg tgaccacgaa
    13561 ggcccgctgc aaccagcggt cggtcccgtc gaagcgagcc tggtacgacg accggccgtg
    13621 gaccgccgcg gtgttgtcga ccaccaggca gtcgccggcc tcgagcacga ccgagcagtg
    13681 gtgctcggcc accgcccgat ccaggcgatc cagggcggcc tgagccgccg ggtcggtacc
    13741 ccgcatcaga tccgcgtcgt accagagggt ggggcgatcg gcggttcccc cgatgacggg
    13801 gaatgggccc cgcggtccga gcggtcccgt cgacgcctcc gacaccgacc ccggcccgga
    13861 ccgccgctcg gtcgatgcga gagcggcccg ggccccgtcg aggaacgact cgtccactcc
    13921 gcagacgaac cgcgcctggc gcaggaccga gagggtgtcg ccgtcgaggg catcgatcag
    13981 gtcgtggacc gagcagaggg tggtccgggc gtcgggatgg ccccgcaggc acagcaggac
    14041 caggaacctc ggcgcgtggg ggtggaaagc cgtctcggtg tggaacgcca ggtccacccg
    14101 cgacgaggtc gacacctggc ggtcggctcc cgcgacggtc gggaccaggt tctggaccag
    14161 atcgccgccg tgctcgggtc ggtagccgac cggctccccc aacgtccggg cgaccgcggt
    14221 cagcacgagc tcgctgacgt ggtccttggt cgtcggctcc gtaggggtcg ccggggtggg
    14281 cgggagctcc ccgaccggaa cgccccggac caggatcgcc ccctcgcggg gcggcgagtc
    14341 gaccatccgg agcagcgcgg agcggaccgt cggcggaagg gtccgtccga gcacctccgc
    14401 ctcggcgacg aacggctcgg atcccggcgc ggccgacgac ggctcgggca cggtcacggc
    14461 cggatcccag gacactgcaa caaccattcc accatgctag cggtgtcgga ccagttgttt
    14521 cagtctcgag cggggagcgg tagccgcgcc tcagtcgctc agcgcatcgg tgtcgaccca
    14581 caccgactcg agcgcgtcca ggcggtccac cgcgctccgg cccatccgga ccgccacccc
    14641 ggccacgagc acctcgccca cggccagcgc cgagacgtag ctgtcgaagg gtccgacgtc
    14701 gtgggactgg accgtcagca ccccgtccgc tccggcggcc accggcgaca gcgacgagtc
    14761 ggtcacggcg atcgtccgga gaccgcgtcc tcgggcccag gccaccacgt cgagcacggc
    14821 ccggtcgtag cgggggagat cgatcacgac caggacgtcg tcgggaccgg accgggcgat
    14881 cgtccgaccg acctcgaccg caccgccgac gagctcctcg accccttcgc gcagcatcga
    14941 caggtgcacc gacacctggt gggccacgcc ggacccggca tcgccaccca tcacgaacac
    15001 acgccggcga cgacccgcca gcagtcggac cgcccgatcg acctccgccg gatcgaggtg
    15061 gcggagggtc cgctccagcg acgccgccac ggatcgcccc aggcccccca ccaggtcgtc
    15121 cgagaccggg cgccggatcc gggcggccgc gggaagcagc cggtgcgaca gatccgcccg
    15181 caccgactcc tgcaggccgc ggaaccccga gaaaccgagc cggtcggcga agcggacgac
    15241 cgtcgccccg ctcgtgccgg cccgctccgc gatgcgcgcc accgtcccga acgcgaccag
    15301 ctcccggtcc gcgaccacga cctcggccac ccgtcgctcg gcggcggaga gcgagcccga
    15361 ggcatccgcc agccggtcgt cgagggagat ggcgccggtg tcagcgggtt cggtcacacc
    15421 ccatattggg ccgcgaccca ccccgacggg gccatcgacg tagccgccga cggaccgttc
    15481 ggcggcacac cgaccgattc cgtaggatgg ccgcttccat aggatcactt atggaggagc
    15541 ccgaccccgg ccacgacggt cggagcgtgc tcctcctcga ccgcacaggg ggacccatga
    15601 ccgaggcctg gatcatcgac gcgtgccgca cgccgcgggg gacgggcaag gtgggcaagg
    15661 gatcgctcgc ccacctccac acccagcggc tgggcgcgac cgtgctcgcc gccctggccg
    15721 agcgcaccgg gctcgacacc ggcgacgtcg acgacgtgat cttcggctgc tcctggcagg
    15781 tcggcaccca gagcggcgac ctcggccgca tggccgccct cgacgccggc tacccgatca
    15841 ccgccagtgg cgtcaccctc gaccggttct gcggatcggg gatcaccgcg gtcaacctgg
    15901 ccgcggccag cgtcatgtcc ggcctcgagg acgtggtggt cgccggcggc gccgagatga
    15961 tgtcgagcta caagcagaac gcccccaaga accgcaaccc gttcatggac aacggcaacc
    16021 tccacctgcg ggcccagcac ccccagacca accagggcgt cgccgccgac gcgatcgcca
    16081 cgctcgaggg catcccccgc tccgccctcg acgctctggc cgccgagtcg cagcgtcggg
    16141 ccgaggtcgc catctccgag ggccgcttcg agcgctcgct cgtgccggtg ctcgacgagg
    16201 atggcaccgt cgccctcgac cgcgaggagt acccccgccc cggcacgacc gtcgactcgc
    16261 tggcacagct ccccgcgagc ttcgaggcgc tcgccgacat gccgctcgac gaggacggca
    16321 ccacgtaccg ggcgatggtc ctggcgcgct accccggcct ggagatcgag cacgtgcacc
    16381 acgccgggaa ctcctccggc gtggtcgacg gcgccgcggc gatcctggtg acgagccccg
    16441 agtacgcgcg ggcccacggc ctgacgccgc gggcacgggt gcgggcgatc gccaacatgg
    16501 gcgacgaccc gaccctgatg ctcaacgccc cggtgcccgc cacccgaaag gtgctcgagc
    16561 gcgccggcat gacgctcgac gacatcgacc tgttcgaggt caacgaggcg ttcgccgtcg
    16621 tccccgagaa gtacatccgc gacctcgaca tcgaccggga gaagatcaac gtcaacggcg
    16681 gagcgatcgc gctcggacac ccgatcgggg ccaccggcag cctgctgatc ggcaccgtcc
    16741 tcgacgagct cgagcgccgc gacctcaacg tcggcctggt caccatgtgc gccgcaggtg
    16801 gcatggcccc cgccgtcgtc atcgaacgca tctgagaacc gaacccgacc cgaccagcag
    16861 gagcaaccga atgtcgatcg ccatcaacga ggaccacgtc gcgctcgccg acaccgtcgc
    16921 cgccttcctg acccagcgcg gctcgctgac cgaggcccgg gcccgcctcg acggcgcccc
    16981 cgaggccctc cccgacttct ggggcgagct ggccgggctc ggctggttgg gcctgcacct
    17041 gggcgaggac gtcggcgggt ccggcttcgg gctcgaggag ctcgtcgtcg tcgccgagca
    17101 gaccggacgc gccctgatgc ccgggccgtt cctgccgacc gtgatcgcct ccgcggccgt
    17161 gcagtccgcc ggcaccgacg agcagcgggc ccgcctgctg cccggcctgg ccgacggctc
    17221 ggtcaccgcc gcggtcgccc tcgacggcga cgtgaccgtt tccgacggga aggcctcggg
    17281 cttcgtgcgc accgcggtcg gcgccgggat cgccgacctg ctgttggtcc cggccggaag
    17341 cgacgtcgtc gtcgtgacgc tgcgcgactc cggtccggcc gccgatccgg tcgccggcgt
    17401 gtcgatcgag gtgcccgaca acctcgaccc cagccgccgc tccggccggg tcaccctgga
    17461 cggcgccccc gccgagaccg tcgccggcgg cgccggcgcg ctggtcgact cggcccggct
    17521 gctgttctcg gccgaggcgg tcggcatcgc ccgggagtgc accgagcagg ccgccgagta
    17581 cgccaaggag cgtcagcagt tcggtcggcc gatcgccatg ttccaggcgg tcaagcacca
    17641 ctgcgccaac atgctcgtcg ccaccgagct ggccaccggc aacgtgtggg acgcggctcg
    17701 caccgaagcg ggcaccgacg ccttctccgc cgctgcggcc gccgctgcga ccctggcgat
    17761 ccccgctgcg gacctgtgcg cccagctgaa catccaggtg cacggcggca tcggcttcac
    17821 ctgggagcac gacgcccaca tgttcctgcg tcgggccacc gcggtcgagc gggtcgtctc
    17881 cgccgaccac gccgcgtccg cgctgaccga cctgcaccgc tccggtgtgc gccgctcggt
    17941 caccatcgag ctgccgcccg aggccgaggc gttccgggac gaggtccgga ccttcgccga
    18001 gcggatcaag ggcctccccg cggatgagca gcgcaccgag ttgatcgact ccggctacgt
    18061 gatgccgcac tggcccgcgc cgtggggtcg cgacgccggg gcggtcgagc agctggtgat
    18121 cgagcaggag ttctccgcgg caggcatcca gaagccgggc tacggcatca ccggctgggt
    18181 gatcctcacg ctgatccagt acgccaccga ggaccagatc gcccgctggg tgcgccccgc
    18241 gctcgagcag gaggtgatct ggtgccagct gttcagcgag cccgacgccg gctccgacgc
    18301 ggcgggaatc accaccaggg ccacccgggt cgacggcggc tggctgctca acggccagaa
    18361 ggtatggacc agcggcgccc acgtcgccgg cttcggcttc gccaccgtgc gtaccgaccc
    18421 cgacgtcccc aagcacgacg gcatcaccgt gatggtcatc gacatgcacg ccgagggcgt
    18481 cgaggtccgg ccgctcaaga tgccgatggg ctcctcggaa ttcaacgagg tgttcttcac
    18541 cgacgtgttc atccccgacg acgacgtggt cggaccggtc gacggcggct ggaccgtcgc
    18601 ccgggccacg ctcggcaacg agagcgtcag catcggcggc ggccagggcg gcatggccat
    18661 gcccgccgag atgctgatcg acgcctacga cgccaacccg acctcgctcg ccgggggctc
    18721 cgagcgcgtc ggccgctacg ccgccaacaa ccgcgcctgc gacatcctga acctgcgcag
    18781 cgcgcaccgg gccgtggccg gcgccgcacc gggccctgaa ggtgcgatca ccaagctggt
    18841 gct
//
LOCUS       DAOQOD010000047        18821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1032_length_18821_cov_11.624161, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000047 DAOQOD010000000
VERSION     DAOQOD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1032_length_18821_cov_11.624161"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1581
                     /locus_tag="PLZ72_06540"
     CDS             220..1581
                     /locus_tag="PLZ72_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNA35990.1"
                     /translation="MSNPYLTGNFAPVTEEMTVADLEVTGSIPEELTGRYLRNGPNPV
                     IAPDPATYHWFLGDGMVHGLRLDGGRARWYRNRWIRSTGVLDALGEPRAADRPDDFGP
                     NTNVIGHAGRTFALVESGPVPYELTEELDTVGPTDFDGTLDGAYTAHPKRDPLTGELH
                     AVAYHWAWGNRVEVTVVDGSGRVAHCRPVETTGSPMVHDMSLTERFAVVYDLPVVFDL
                     DAAMAGSTLPYNWSDDYPPRIGFVPRADTVDATGDVVWVDVDPCFVFHPMNAHDVVGA
                     DGRVESVVLDVVRWGRMFDRGHQGPFESGRPELVRWTVDLARRTVSAAPLSDLDLEFP
                     RVDERRIGRPCRFGYTAVNLAGSGGVAHGGIARHDLRDGTVEVLDLGVGAGQNEAVFV
                     PASPDADEDDGWVLCLTYGADTDTSRLEIRHAQALTDGPVATVHLPVRVPFGFHGNWI
                     PDA"
     gene            complement(1591..3156)
                     /locus_tag="PLZ72_06545"
     CDS             complement(1591..3156)
                     /locus_tag="PLZ72_06545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNA35991.1"
                     /translation="MLGRRQFLVASGAAVGAAALGATSACSPAGGPVPGVFGLGVASG
                     LHSPTEVVLWTRVELANAPGTTTVDWELADTASLEHVVASGTAPVSAASDGCVKVLVD
                     GLDPDRSYWFRFRSAGTTSTVGRARTLPAAGSSPTSLRLAFAACQSYASGFYGAWRDI
                     AAQDLDAVLFLGDYIYESFPIQLQRRLRDEYLEDADTLERYRQRYRLYRSDPDLQAAH
                     AAHPIVPTWDDHEVANDHDRAFLAEFPERGAAAHQAWFEYMPVWPIDGSRVFRDLRWG
                     DLAQLFVLDCRQYRDEHLGTALLGLLGGTVMSDDLQAVGRTMLGAPQRQWLLDGLASA
                     QQTGVRWKLVGNPVMIAPVRLVDMDTPEMRALDLSLPRHAGLYLGTDSWDGFGWERDL
                     VLGHLRDHSITDVAFLTGDQHTFMAAPLSTDFDDPAAPTVAYEYTGGSISSPCGITEE
                     LLSGGLERYSTSPPFDFMDIHRNGYGLVELTPDAMSVTFRALDATSPAATPVPSVRFD
                     TLVGDPQPQVTRL"
     gene            complement(3325..4194)
                     /locus_tag="PLZ72_06550"
     CDS             complement(3325..4194)
                     /locus_tag="PLZ72_06550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA35992.1"
                     /translation="MRFTYAEALTNPSYMAPLAQAADAAGYDAFLVPDSVAYPEVSNS
                     IYPFTPDGSREFLEDKPLLEPFALISALAMVTERIRFTVSVLKLPVRHPLHTAKLAST
                     AAVLSGNRLNLGVGVSPWPEDYEMTGTPWEGRGRRMDECIDIVSSLLDGGYHEHHGEF
                     FDLPSLKICPVPTQKVPILVGGHSGPALRRAARLDGWIHGGGSDDLDELIERVKQLRR
                     ESDRADEPFQIQVISAEGFSVDGVRRLEDKGVTDVIVGFRWPYVVGADTEPLADKIGA
                     LERFAETTIAACR"
     gene            complement(4398..5321)
                     /locus_tag="PLZ72_06555"
     CDS             complement(4398..5321)
                     /locus_tag="PLZ72_06555"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA35993.1"
                     /translation="MKHRNLLAVGLIAAVALFAGACSGDPGGPATPANTPPVANIDTG
                     GVTTGLAPLTIAFDATGSVDTDGTIVNYYWDFGDSHTQNGDVVSHTFAPGTWTVQLKV
                     TDDKGAIGTTATTIVATNNAPTADFSISPATGTAPLNATFDASILSGDIDGTIVSYAW
                     DFQELGTESGATVVKTIPAGTIDVTLTVTDNNGATATKTRSVTATGAPATAPTGFTKT
                     GSGCCPTYGDFSWNPVPGADSYDIFLDPTFGCTWFGHTYQQSFPGQVSTAHFVDGGAT
                     GMCLGTQYDAKIRAVANGTAGPWSATIHFTL"
     gene            complement(5389..6810)
                     /locus_tag="PLZ72_06560"
     CDS             complement(5389..6810)
                     /locus_tag="PLZ72_06560"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA35994.1"
                     /translation="MNRSVRLRRVAALIAVPVVAVLVAASCSTPPGTPAPNYGFKFRA
                     SKVTVVNHNDTFFYGTRDEPFVYNLWFRVKVGVPGSAQVGIVGDRAQAFDDLGDGQSH
                     VLVGGERAEVDFPNVQLLDVLDLANPVNGLEVVGTWTWAMEQDDVTVLPVAQAAAGVM
                     ENALDATVAAGSVPSDPNALISQLFDDFGQAFTLIAGGLFDSIPGIPDDAIGSRFYVG
                     VAAKGSLAGIINGANVNFPTVDIPVISVPPNIDGGMIFALDGGRNIGGQQFSSSGARH
                     DYDLQVINTATLNKPPVASATASVTSGSAPLAVAFNGAGSSDPDGTVVAYDWNFGDFT
                     TGSGAITSHTFTQPGTYPVTLTVTDNRGDATSTTVTVTVTGAPTVAPTNLQKVGSGCC
                     DTYGDFAWTPVPGADGYEVFMDGVFGQGCFTDHGAVINGQTNHGRVQAVGLCLGSDYE
                     VKIRARANGQWGPWSPIVYVNNL"
     gene            7064..8782
                     /locus_tag="PLZ72_06565"
     CDS             7064..8782
                     /locus_tag="PLZ72_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA35995.1"
                     /translation="MLSYDEAARQVTAAGMPFETVQQDVMGVPRTLFRNAPNSLRDIV
                     VAASARSDDVTFLVYEDERWGFERFGREVSALGTALVEQYGIGRGDRVAIAMRNYPEW
                     VVSFAAAVSIGAISVSFNAWWTAEEMDFALSDCGASVLIADAERIERSRATCERLGVP
                     PLAVRAPDAAALGTTAAPVAQWSDVVGARATDPTVALPDVAIDPDDDATILYTSGTTG
                     NPKGAVSTHRAVVQALMGYACRSALDKARDPDRQGGLAAPSFILVVPLFHVTGCVPVM
                     LSCMLGGLKLVMVYRWDPERALQLIEREEISNFVGVPTQNFDMLNCEAFERYDTSSLR
                     SVGGGGAPAPPELVQRIATTYAKAAPGIGYGMTETNAYGPQNGGADYLTHPTSAGRTT
                     PIMAVEIRDPSGVPVPVGEVGEIWFNGPNLIRGYWNRPDATAEVLVDGWLRSGDVGRL
                     DAEGFVYVEDRIKDMVLRGGENVYCAEVEAAIHEHPAVHEVAVFGVPHDRLGEEVAAV
                     VVPRAGASVTETELVDFVAERLAPFKVPTRLRIRPEPLPRNAAGKFLKRDLRREEEAA
                     SASATA"
     gene            8854..9897
                     /locus_tag="PLZ72_06570"
     CDS             8854..9897
                     /locus_tag="PLZ72_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNA35996.1"
                     /translation="MDAATKTNPGNFFEDFTLGQRIDHATPRTVTAGDRALYGALYPT
                     RFALPSSDEFAAACGLSPAPVEELIGFHIAFGKTVPDISLNAVANLGYAECRFRRPVR
                     PGDTLRSSSEVIGLKENSNGRTGVVWVRSTATDQHGRTAIDWARWVMVRKRDESAPAP
                     EAVVPDLAPFVEPADLIVPDGLDLTAYDAVAAGSPFRFDDYEVGEKIDHVDGVTLSEA
                     EHMMATRLWQNTAKVHFNTEARDDGRRLIYGGHVISMARALSFNGLANAQLIAAINAG
                     AHTNPAFADDTVYAWSEVLDKAELDAPGVGALRLRLVATKGRDESMTLKGDDGKYLPH
                     VLLDLDLWVLCPR"
     gene            complement(9935..10462)
                     /locus_tag="PLZ72_06575"
     CDS             complement(9935..10462)
                     /locus_tag="PLZ72_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HNA35997.1"
                     /translation="MAIDTFYAYLGVYDDPADALADYEAVHALHTEAGLIDAYDAAVV
                     EKRDDGKVKIVKKHETPTRVGGVLGGGVGLATGLVIALFPAAAIGTGLLVGTTAGGAL
                     LGSLAGHAAAGMSRKDLKEAGEQLDEGTAGLVVVAVADMQSKIEHAMKKARKVEEKQL
                     KADSEAIEAEATEKS"
     gene            complement(10578..11411)
                     /locus_tag="PLZ72_06580"
     CDS             complement(10578..11411)
                     /locus_tag="PLZ72_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA35998.1"
                     /translation="MDPATPSTTETDSRSAPPTFDTRRVRAMVRRSLLSQSRNPDFWF
                     LLLVLPLVDGLLFGSIGVAYGIGDAPVVLLVTGIMLFHLIWQLTLAGSFGLLDEVWSR
                     NLLNLIATPLTEREMLSSFGVVGLLRTGVSVTVIGAVGVVFFAVSPTSAGVVLVPSAA
                     LLLLFGWSVALFVVGLTLQYGDRAEVFSWGTLVLLMPLSGVFYPVESLPPVLQVVARA
                     IPLTHVFDAVRIGIEDGEVAWAQLAVAAAGTAVIAAASTWFVAHQLRRFRRDGWVTRF
                     V"
     gene            complement(11411..12178)
                     /locus_tag="PLZ72_06585"
     CDS             complement(11411..12178)
                     /locus_tag="PLZ72_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA35999.1"
                     /translation="MEQPTTPTGPDVVLRASRLRKDFGDTRAVDDVSITIHAGERVGI
                     VGPNGAGKTTTLLMLLGAIEPDHGTIDIAGHRLPGGRSDAMTRVGFAAGYLPLPSGLT
                     VTETLELFGRLYGVADPTTRAAEVLDELHIDRLAGQKVESLSSGQGTLVGFAKAVIHH
                     PRLIVLDEPTASLDPDVAMRVRDRLEHMNSERNATLLLTSHDMREVAELTTRVIFLRQ
                     GRIIADGPPDQVVASHGHADLESMFLAEAARVREQEV"
     gene            12368..12730
                     /locus_tag="PLZ72_06590"
     CDS             12368..12730
                     /locus_tag="PLZ72_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNA36000.1"
                     /translation="MAQYLLSVWHDEDYIDAFETTPPEVIERLHAQVGALNADMEKKG
                     VWVFGGGLVPASSATVVRPGTDGDVSITDGPYVEAKEMMGGFWVIEAPDLDSALEWTR
                     RAALACEGAVELRPFQDE"
     gene            12723..14138
                     /locus_tag="PLZ72_06595"
     CDS             12723..14138
                     /locus_tag="PLZ72_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNA36001.1"
                     /translation="MSESGARAARSASSAGPAAELSNAVESVFRRESGRCTATLIRVL
                     GDVDLAEDAVAEAFEVALTRWPRDGVPDNPGAWITTTARRRALDRLRRESTRHDRHVA
                     AHRTFGTGDPTGADPSTVGRLVEELGGEQVEVVADDQLRLMFLCCHPVLAPDSQVALT
                     LRLLGGLQTPEIARAFLVPEATMAQRIVRAKRKLRDNHARYRIPVEAELPDRLPPVLA
                     AISLIFTEGHRATDGSGLVRVELSNEAIRLGRVLADLMPDEPEAAGLLALMLLTDARR
                     SARLDAAGALVPLAEQDRDRWDQDRIAEGLDRLAPWSRRVDPGRFRIQAELAAAHARA
                     ATFADTDWARIVELYDVLLQRRSDPVVAINRAVALGELDGPAAALLALEGLTTTSVSS
                     STPTSRGTPPGPISWPGPGVRPRPSRRTTGRSPSPPRRRSGGSSPLVARRAEPGRPRR
                     SRPEVGCGVDIGRGAPTGGST"
     gene            14135..15187
                     /locus_tag="PLZ72_06600"
     CDS             14135..15187
                     /locus_tag="PLZ72_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNA36002.1"
                     /translation="MTDTAARNDTATRNDTATRNVTADREDRPPPPDRADPTAIGAQR
                     VALPGSDVKVGPLGHLAAERQRLDALRWEHFDVTRTSATLGAEVSGISLADDLPDPVI
                     ADLARALADYKVLFFRDQPLTTAQHVAFARRFGELEVHPFLPANTDSPEVVRFAKSAD
                     VGGYENGWHHDVTWREVPSMGAVLHAVEVPDSGGDTLFADAHAALDELEPQLRERVDG
                     LLAVHDFVHVFGHTLSPDDLASMRELHPPVAHPVIATHDRTGRDHLYVNRFFVSHLTE
                     ADGEPLDPAESAQLIDALCRPFETPELQVRLRWEPDSVAFWDNRAVQHYACSDYWPDV
                     RVMERASIVGTRPVRR"
     gene            15296..16363
                     /locus_tag="PLZ72_06605"
     CDS             15296..16363
                     /locus_tag="PLZ72_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNA36003.1"
                     /translation="MGSYTASAVAAIVGGVIVAVVIFVPDVAYRYRRSGRLRGSDVVA
                     VVVVPTYALALWTYTLLPLPDPDDIVCRDPNLDAFRFVDQIRASGVDSLADLLTNWAF
                     ASTVLNVLLFVPLGVILRWRFRRGVLVALVVGFAVSLLIETTQLTGLWGVYDCAYRYF
                     DVDDLITNTTGAVVGSVLSWLVLRHRPDPEVVPDEAMATSGQRIVAVACDVVVMLLVG
                     TATVVAWRAIALRTLKIDEAVHPLVQRSLQWGVPLALEAIAVVGFGRTIGEWAVRLGT
                     VARRPGWTLPGRVLKLAFGPGLIAAVGWWDSGWSGVLLFVLAALSVVVAVVTPDHRGL
                     ANTVSGLRPVVTPWGRPADPD"
     gene            16389..17417
                     /locus_tag="PLZ72_06610"
     CDS             16389..17417
                     /locus_tag="PLZ72_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA36004.1"
                     /translation="MAPTFLTEFDPRYGEPVAVSDGVVRVVADNPSKYTAWGTGTYVV
                     GEAQVAVIDPGPDLPAHVDALLAALDGRAVSHILITHTHADHSPAAAALAAATGAPTY
                     GFGPHPIDAGTGDDAVDGPDAADRPDAADRPDAADGGEETESGDRDFVPDVVVRDGDV
                     LVGDGFRFECLHTPGHISNHVCFAEVERNLLFPGDHVMGWSTTVVSPPDGDMDAYIAN
                     LRRLVDRHAAGRDAIYLPTHGSAIVDPGPFVESLIEHRLERDRQILDLLAAAPAGDGV
                     SVEGLVAVMYADVRPELHRPAGRSVLAHLIGLAGRGSVERRPDGRWQLRRRPGQSGAM
                     TVTGPVLP"
     gene            17441..18466
                     /locus_tag="PLZ72_06615"
     CDS             17441..18466
                     /locus_tag="PLZ72_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNA36005.1"
                     /translation="MTGPGSATPPGSAAPPAAAVGPSPSPGTSAEPPTGPDVRNGISL
                     AVASAACFGLSGSLASALMLSGWTAGAAVTARVMIAALVLVVPGVRAAGVDGRDRWAV
                     LRRNAGTIVAYGVLAVAATQLCYFYAVSHLPVGVALLVEYTAPVGVVGWMWAVHRQRP
                     SRLTVLGAAFALCGLPLLLDLLGSAGSGGGVDPVGVAWALAAMVGAAAYFVMSADDRS
                     GLPPITLAASGLVVGAVVLGAAGLLGVLPVTATTDDVAFQRLVVPWWVPVLALGVVTA
                     AVAYVTGIAATRRLGSRLASFVALTEVIAATAFAWLLLGQTPLPVQLVGAAVVLGGVA
                     VVRLGEP"
     gene            complement(18565..>18821)
                     /locus_tag="PLZ72_06620"
     CDS             complement(18565..>18821)
                     /locus_tag="PLZ72_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36006.1"
                     /translation="AVAGAAPGPEGAITKLVLSENLAEAAAVLNATNPDAAYLDAAGL
                     TSGALVLWHRGMSIAGGTSEIKRNQIGERILGLPRDPLIK"
BASE COUNT         2627 a         6450 c         6941 g         2803 t
ORIGIN      
        1 cggagggaga cctggaggcg gctccagcac caggccccct gccaggcccc gtggatgagg
       61 acgaacatgt cccgaaccgt accggtcccg atacctgtcg ggagcagcct gggcgtgcgc
      121 accgatgtcg acgcggtggg gtcgctacgt tgagctgatc gaaccggcgc acacgagggg
      181 cgcccggttc tcaaacgtcg atgccgcgga ggtctgatcg tgagcaatcc gtacctgaca
      241 ggcaacttcg ctccggtgac cgaggaaatg accgtcgccg acctggaggt caccgggtcg
      301 atccccgagg agctcaccgg ccgctacctg cgcaacggtc cgaacccggt gatcgcgccc
      361 gatccggcca cctaccactg gttcctgggc gacgggatgg tccacggcct gcgcctcgac
      421 ggtggccgcg ctcgctggta ccggaaccgc tggatccgca gcaccggcgt gctcgacgcc
      481 ctcggtgaac cccgcgccgc ggatcgaccc gacgacttcg gcccgaacac caacgtgatc
      541 ggccacgccg ggcgcacctt cgccctggtc gagtcgggac ccgttcccta cgagctcacc
      601 gaggagctcg acacggtcgg accgaccgac ttcgacggca ccctcgacgg cgcctacacc
      661 gcgcacccca agcgcgatcc gctcaccggg gagctgcacg cggtcgccta ccactgggcc
      721 tgggggaacc gcgtggaggt gacggtggtc gacgggtccg gtcgggtggc gcactgccgg
      781 ccggtggaga cgaccgggag cccgatggtg cacgacatgt ccctgaccga gcggttcgcc
      841 gtggtctacg accttccggt cgtgttcgac ctcgacgccg cgatggcggg ctcgaccctc
      901 ccgtacaact ggtccgacga ctatccgcct cggatcggct tcgtcccccg agccgacacg
      961 gtggatgcga cgggcgacgt cgtgtgggtc gacgtcgacc catgcttcgt gttccacccc
     1021 atgaacgccc acgacgttgt cggcgccgac ggtcgggtcg agtcggtcgt gctcgatgtc
     1081 gtgcggtggg gccgcatgtt cgaccgcggg caccagggcc cgttcgagag cggtcgcccc
     1141 gagctggtgc gctggaccgt cgatctggcg cgacggacgg tgagcgcggc tccgctcagc
     1201 gacctcgatc tggagttccc ccgggtggac gagcgccgga tcggtcgacc gtgccggttc
     1261 ggctacaccg cggtgaacct tgcggggtcg ggcggggtgg cgcacggcgg gatcgcccgc
     1321 cacgacctgc gtgacggcac cgtcgaggtg ctcgacctcg gagtcggcgc cggccagaac
     1381 gaggcggtgt tcgtccccgc atcacccgac gccgacgagg acgacggctg ggtgctgtgc
     1441 ctcacctacg gcgctgacac cgacacgagc cggctggaga tccgccacgc ccaggccctc
     1501 accgacggcc ccgtcgccac ggtgcacctg ccggtgcggg ttccgttcgg gttccacggg
     1561 aactggatcc cggacgcctg accgccggga tcagaggcgc gtgacttgcg gctgcgggtc
     1621 gcccaccagc gtgtcgaagc ggaccgaggg aaccggcgtg gcggccggtg acgtcgcgtc
     1681 gagtgcccgg aacgtgaccg acatcgcgtc cggggtcagc tcgacgagcc cgtacccgtt
     1741 tcggtggatg tccatgaagt cgaacggcgg actcgtgctg taccgctcga ggccacccga
     1801 gagcagctcc tcggtgattc cgcacggcga ggagatcgac ccgccggtgt actcgtaggc
     1861 gacggtcggc gccgcggggt cgtcgaagtc ggtgctgagc ggtgcggcca tgaacgtgtg
     1921 ctggtctccc gtgaggaacg ccacgtcggt gatcgagtgg tcgcgcaggt ggccgagcac
     1981 caggtcgcgc tcccagccga agccgtccca cgagtcggtg cccagataca ggccggcgtg
     2041 acggggcagc gacaggtcca gggcgcgcat ctccggcgtg tccatgtcga cgaggcggac
     2101 cggggcgatc atcaccgggt tgccgaccag cttccagcgc acaccggtct gctgggccga
     2161 ggcgaggccg tcgaggagcc actgcctctg cggcgcgccc agcatcgtcc gaccgaccgc
     2221 ctggaggtcg tcgctcatga ccgtcccgcc cagcagcccg aggagcgccg tgccgaggtg
     2281 ctcgtcgcgg tactggcggc agtcgaggac gaacagctgg gccaggtcgc cccaacgcag
     2341 atcccggaac acccggctgc cgtcgatcgg ccacaccggc atgtactcga accacgcctg
     2401 gtgcgccgcg gcgccgcgtt cggggaactc ggccaggaac gcccggtcgt ggtcgttggc
     2461 cacctcgtgg tcgtcccacg tcgggacgat cggatgcgcg gcgtgggcgg cctgcaggtc
     2521 ggggtcgctg cggtacaggc ggtagcgctg gcggtagcgc tcgagggtgt cggcgtcctc
     2581 gaggtactcg tcgcgcagac ggcgttgcag ctggatcggg aacgactcgt agatgtagtc
     2641 gccgaggaac agcaccgcgt cgaggtcctg ggcggcgatg tcgcgccacg ccccgtagaa
     2701 gccgctggcg taggactggc acgcggcgaa cgccagtcgc agcgacgtcg gtgaggatcc
     2761 cgcggcgggc agggtgcggg cccgcccgac cgtcgaggtc gtcccggcgg agcggaagcg
     2821 gaaccagtag gagcggtcgg ggtccaggcc gtcgaccagc accttgacgc acccgtcgga
     2881 cgcggcgctg accggggcgg tgccggaggc gacgacgtgc tcgagcgagg ctgtgtcggc
     2941 cagctcccag tcgaccgtgg tggtgccggg agcgttggcc agctcgacgc gggtccacag
     3001 gaccacctcg gtcggggagt gcaggcccga ggcgacgccc aggccgaaga cgccgggaac
     3061 cggaccgccg gccgggctgc acgcacttgt cgctcccagt gcggcggctc ccaccgccgc
     3121 gccgctcgcg accaggaact gccttcgacc cagcatctcg actcccgctc ttcgcctgag
     3181 cgaccaggct acgcgtcccg ccgggcacac cccgaatccg attcaggatc ccctcccgtc
     3241 cttcccttcc cgttctgtag tgcgagatcc aggcgaacgc ctggatctcg cactacagaa
     3301 cgggggtcag cggcagaacg ggggtcagcg gcaggcggcg atcgtggtct cggcgaaccg
     3361 ctcgagtgcg ccgatcttgt cggcaagcgg ctccgtgtcc gcgccgacga cgtacggcca
     3421 gcggaagccg acgatcacgt cggtcacgcc cttgtcctcc aggcgacgga cgccgtcgac
     3481 gctgaagccc tcggcgctga tcacctggat ctggaacggc tcgtcggccc gatccgactc
     3541 gcggcgcaac tgcttcaccc gctcgatcag ctcgtcgagg tcgtcggagc cgccgccgtg
     3601 gatccagccg tcgaggcggg cggcgcgacg gagcgcggga ccggagtggc cgccgaccag
     3661 gatcggcacc ttctgggtgg ggaccgggca gatcttcagc gacggcaggt cgaagaactc
     3721 gccgtggtgc tcgtggtagc cgccgtcgag caggctcgac acgatgtcga tgcactcgtc
     3781 catgcggcgg ccgcgaccct cccacggagt tccggtcatc tcgtagtcct cgggccaggg
     3841 gctgacgccg acgccgaggt tgagccggtt gcccgacagc accgcggcgg tcgaggcgag
     3901 cttggcggtg tgcagcgggt ggcgcaccgg cagcttgagc accgagacgg tgaagcggat
     3961 gcgctcggtg accatggcca gggcgctgat cagagcgaac ggctcgagca gcggcttgtc
     4021 ctcgaggaac tcgcgggagc cgtcgggggt gaacggatag atcgagttcg acacctcggg
     4081 gtaggcgacc gagtcgggga cgaggaaggc gtcgtagccc gcggcgtccg cggcttgggc
     4141 cagcggcgcc atgtagctcg ggttggtcag cgcctcggcg taggtgaacc gcaactgtgc
     4201 cccctcgggt gatccgccgg ccggtttggc cgtcgggggc tcatcgtgtc acgcgtgcgg
     4261 ggtggcggtg gtcagcgccc gggtcggaac cgagacgcga gccgcatgcc gatccgatcg
     4321 ctgctcggac gcgacggtgg ccgcaccctg gaggggtgcg gccaccgggt cagtcgatca
     4381 ggatcgggcc ggtcgactca gagggtgaag tggatcgtgg cggaccaggg gccggcggtg
     4441 ccgttcgcca ccgcacggat cttggcgtcg tactgcgtgc ccaggcacat tccggtcgct
     4501 ccgccgtcga cgaagtgggc tgtcgagacc tgaccgggga acgactgctg gtaggtgtgg
     4561 ccgaaccagg tgcatccgaa ggtcggatcc aggaagatgt cgtaggaatc cgcgccgggg
     4621 accgggttcc acgagaagtc gccgtaggtc ggacagcagc ccgagcccgt cttggtgaag
     4681 ccggtcgggg cggtcgccgg ggcgccggtc gcggtgaccg aacgggtctt ggtggccgtg
     4741 gcgccgttgt tgtcggtgac ggtcagggtc acgtcgatgg tcccggcggg gatcgtcttg
     4801 acgaccgtcg cgccggactc ggtgccgagc tcctggaagt cccaggcgta gctgacgatc
     4861 gtgccgtcga tgtcgcccga caggatcgag gcgtcgaagg tggcgttcag cggggccgtc
     4921 ccggtcgccg gcgagatcga gaagtcggcg gtcggggcgt tgttggtggc cacgatggtg
     4981 gtcgccgtgg tgccgatcgc gcccttgtcg tcggtgacct tcagctgcac cgtccacgtc
     5041 ccgggggcga aggtgtggct cacgacgtcg ccgttctggg tgtggctgtc gccgaagtcc
     5101 cagtagtagt tgacgatggt gccgtcggtg tcgaccgagc cggtggcgtc gaaggcgatt
     5161 gtcagcggcg ccagacccgt ggtcacgccc ccggtgtcga tgttcgccac cggcggggtg
     5221 ttggccgggg tggccggtcc gccggggtca ccggagcacg ctccggcgaa cagggcgacg
     5281 gccgcgatca ggccgacggc gagcaggttg cggtgcttca tggatctccc tccagggctg
     5341 aacgatgtgt cgtgtggggt gggcgggtgc cgagtcggtc cgacggcgtc agaggttgtt
     5401 gacgtagacg atcggggacc acggacccca ctggccgttg gcccgagccc ggatcttcac
     5461 ctcgtagtcg gaaccgaggc agaggccaac ggcctggacc cgcccatggt tcgtctggcc
     5521 gttgatcacc gcaccgtggt cggtgaagca gccctggccg aacacgccgt ccatgaagac
     5581 ctcgtagccg tcggcgccgg gcaccggggt ccaggcgaag tctccgtagg tgtcgcagca
     5641 gcctgagccc accttctgca ggttggtggg ggcgacggtc ggggcgccgg tgacggtgac
     5701 ggtcacggtg gtcgaggtgg cgtcgccgcg gttgtcggtg acggtgaggg tcaccgggta
     5761 ggtgccgggc tgggtgaagg tgtggctggt gatcgccccg gaaccggtgg tgaagtcgcc
     5821 gaagttccag tcgtaggcga caacggtgcc gtccgggtcg ctcgagccgg ccccgttgaa
     5881 cgccaccgcc agcggcgcgc tgcccgaggt caccgacgcg gtggccgagg cgaccggcgg
     5941 cttgttcagg gtggcggtgt tgatcacctg caggtcgtag tcgtggcggg cgccgctcga
     6001 ggagaactgc tggccgccga tgttgcggcc gccgtcgagg gcgaagatca tgccgccgtc
     6061 gatgttcggt gggacgctga tgaccgggat gtcgacggtc gggaagttca cgttggcgcc
     6121 gttgatgatc ccggccaggg agcccttggc cgcgactccg acgtagaagc gggagccgat
     6181 ggcgtcgtcg gggatgccgg ggatggagtc gaacaggccg ccggcgatca gggtgaacgc
     6241 ctggccgaag tcgtcgaaga gctgggagat cagggcgttg gggtcgctgg ggacggagcc
     6301 ggcggccacg gtggcgtcga gggcgttctc catgacgccg gcggcggcct gcgcgaccgg
     6361 cagcacggtc acgtcgtcct gctccatcgc ccaggtccag gtcccgacga cctcgaggcc
     6421 gttgaccggg ttggccaggt ccaacacgtc gagcagctgc acgttgggga agtcgacctc
     6481 ggcgcgctcg ccgccgacca ggacgtggga ctgtccgtcg ccgaggtcgt cgaaggcctg
     6541 ggctcggtcg ccgacgatgc cgacctgagc ggatccgggg acgccgacct tgacccggaa
     6601 ccacaggttg tagacgaacg gctcgtcgcg ggtgccgtag aagaacgtgt cgttgtggtt
     6661 gacgacggtg accttgctgg cccggaactt gaacccgtag ttgggtgcgg gagtccccgg
     6721 aggtgtcgag caggacgccg ccaccagcac ggcgaccacg gggacggcga tcagggcggc
     6781 gacgcggcgc agccggacag agcggttcat ggcggggttc tccctcgtcg gcgcggggac
     6841 gcccccacgt tcgcaacgga cctcctgagc cgggttcagg aggtcttgaa cgatagttca
     6901 ggaacgggtt cgcgaacaag gtgggtggcg ggcaattggc ccatcccgac gggccggggc
     6961 acggccgctc ggaccgcacg ggatcggatc cggtcagcga tgagcgagca gcggcaggtg
     7021 ctcgagacgt cgcggggtcc gaagcatctg cgatgctctc gggatgctca gctacgacga
     7081 ggcggcccgg caggtgaccg cggccgggat gccgttcgag accgtgcagc aggacgtgat
     7141 gggagtgcca cgcacgttgt tccgcaacgc tccgaacagc ctgcgcgaca tcgtcgtcgc
     7201 cgcgtccgct cgcagcgacg acgtcacctt cctggtctac gaggacgaac ggtggggctt
     7261 cgagcgcttc ggtcgcgagg tctcggccct cgggacggcc ctggtggagc agtacggcat
     7321 cggacgcggc gaccgggtgg cgatcgccat gcgcaactac cccgagtggg tcgtgtcctt
     7381 cgccgccgcg gtgtcgatcg gggcgatctc ggtcagcttc aacgcctggt ggaccgccga
     7441 ggagatggac ttcgccctgt ccgactgcgg ggcgtcggtg ctgatcgcgg acgccgagcg
     7501 gatcgagcgc agccgggcca cgtgcgagcg actgggggtt ccgcccctcg ccgtgcgcgc
     7561 tccggacgcg gccgcgctcg gtaccaccgc cgcaccggtc gcccagtggt ccgacgtcgt
     7621 gggagcccgg gccaccgatc cgaccgtcgc cctgcccgac gtggcgatcg atcccgacga
     7681 cgacgccacg atcctctaca cgtcgggcac gacgggcaac cccaagggcg cggtctccac
     7741 ccaccgcgcc gtcgtgcagg cgctgatggg ctacgcgtgc cgctccgcgc tggacaaggc
     7801 gcgtgacccc gaccgtcagg gcggactggc cgctccgtcg ttcatcctgg tcgtcccgct
     7861 gttccacgtg accggctgcg tcccggtcat gctcagctgc atgctcggcg ggctgaagct
     7921 ggtgatggtc taccggtggg atcccgagcg ggcgttgcag ctgatcgaac gggaggagat
     7981 ctcgaacttc gtcggcgtcc ccacccagaa cttcgacatg ctgaactgcg aggcgttcga
     8041 gcgctacgac acctcgagcc tgcgcagcgt cgggggcggg ggagcgccgg ccccgccgga
     8101 gctggtgcag cgcatcgcga ccacctacgc gaaggccgcg ccgggcatcg gctacgggat
     8161 gaccgagacc aacgcctacg gaccccagaa cggcggggcc gactacctga cgcacccgac
     8221 cagcgccggg cggacgacgc cgatcatggc ggtcgagatc cgcgacccga gcggcgtgcc
     8281 ggtccccgtc ggcgaggtcg gtgagatctg gttcaacgga ccgaacctga tccgcggcta
     8341 ctggaaccga cccgacgcca ccgccgaggt gctcgtcgac gggtggctgc gcagtggcga
     8401 tgtcggccga ctcgacgcgg agggcttcgt ctacgtcgag gaccggatca aggacatggt
     8461 gctgcgaggc ggcgagaacg tgtactgcgc cgaggtcgag gccgcgatcc acgagcaccc
     8521 cgccgtgcac gaggtggcgg tcttcggcgt gccccacgac cgcttgggcg aggaggtcgc
     8581 ggcggtcgtc gtgccaaggg ccggcgcgtc ggtgactgag accgagctgg tcgacttcgt
     8641 ggcggaacgt ctggcgccgt tcaaggtgcc gacccgtctg cggatccggc ccgagccgct
     8701 gccgcgcaac gcggccggca agttcctcaa gcgcgacctg cgccgcgagg aggaggcggc
     8761 ctcggcctcg gcgactgcgt gacggcggcg gctcgacgtc ggctcgacgg cggcttgatg
     8821 gcggcggcgg gaccccgggt accgtgagcg ccgatggacg cagcgaccaa gaccaacccg
     8881 ggcaacttct tcgaggactt caccctcggg cagcggatcg accacgccac cccccgcacc
     8941 gtcaccgccg gtgaccgggc gctgtacggc gccctgtacc cgacgcggtt cgccctgccg
     9001 tcctccgacg agttcgcggc cgcctgcgga ctcagcccgg cgccggtcga ggagctgatc
     9061 ggcttccaca tcgcgttcgg caagacggtg ccggacatct cgctcaacgc cgtggccaac
     9121 ctgggctacg ccgagtgccg cttccgccga ccggtgcgcc ccggagacac gctgcggtcg
     9181 agctctgagg tgatcggtct caaggagaac tccaacggtc gcaccggtgt cgtgtgggtg
     9241 cggtcgaccg ccaccgacca gcacggtcgc accgccatcg actgggcccg ttgggtcatg
     9301 gtccgcaagc gggacgagtc ggcccccgcc cccgaggccg tcgtgcccga cctggcgccg
     9361 ttcgtggagc cggccgacct gatcgtgccc gacggactcg acctcaccgc ctacgacgcg
     9421 gtggcggccg gttcgccgtt ccgattcgac gactacgagg tgggcgagaa gatcgaccac
     9481 gtcgacggcg tcaccctgtc ggaggccgag cacatgatgg cgacccggct gtggcagaac
     9541 acggccaagg tccacttcaa caccgaggcc cgcgacgacg gccgccgcct gatctacggc
     9601 ggccacgtca tctcgatggc gcgggccctg tcgttcaacg ggctggccaa cgcccagctg
     9661 atcgcggcga tcaacgcggg cgcgcacacc aacccggcct tcgccgacga caccgtctac
     9721 gcgtggtcag aggtgctcga caaggccgag ctcgatgccc ccggcgtcgg ggcgctgcgt
     9781 ctgcggctgg tggccacgaa gggccgcgac gagtcgatga cgctcaaggg cgacgacggc
     9841 aagtacctgc cgcacgtgct gttggacctg gacctctggg tgctctgccc ccgctgagtg
     9901 ctgtgccccc gctgaccgag ggctcgctcc ggggtcagga cttctcggtg gcctcggcct
     9961 cgatcgcctc ggagtcggcc ttcagctgct tctcctcgac cttgcgggcc ttcttcatgg
    10021 cgtgctcgat cttggactgc atgtcggcca ccgcgaccac gaccaggccg gcggtgccct
    10081 cgtcgagctg ctcgccggcc tccttgaggt ccttgcggct catgcccgcg gccgcgtgcc
    10141 cggcgagcga acccaggagt gctcctccgg cggtggtgcc gaccagcaat cctgtgccga
    10201 tggcggccgc cgggaagagg gctatcacca gaccggtggc cagcccgacg ccaccgccga
    10261 gcacgccgcc gacccgggtg ggtgtctcgt gcttcttgac gatcttgacc ttgccgtcgt
    10321 cgcgcttctc gaccacggcg gcgtcgtagg cgtcgatcag gcccgcctcg gtgtggaggg
    10381 cgtggaccgc ctcgtagtcg gcgagggcgt cggcggggtc gtcgtagacg ccgaggtagg
    10441 cgtagaaggt gtcgatcgcc attgggagct ccgttccgtc ggtccgccgg cgggtgcccg
    10501 ccgcggtacc ggtgcagcct acggaggtcg gccggcggtc gtcgtcgccc cgaacgggtg
    10561 atccgacgga gcggggctca gacgaagcgg gtcacccagc cgtcgcggcg gaaccggcgg
    10621 agctggtggg ccacgaacca ggtcgaagcc gccgcgatga ccgcggtgcc ggcagcggcg
    10681 accgcgagct gggcccatgc cacctcgccg tcctcgatgc cgatcctcac cgcgtcgaac
    10741 acgtgggtga gcgggatggc tcgggcgacg acctgcagga ccgggggcag cgactcgacg
    10801 gggtagaaca cgcccgacag cggcatcagc agcacgagcg tcccccacga gaacacctcc
    10861 gcccgatcgc cgtactggag cgtcaacccg accacgaaca gggcgacgga ccagccgaac
    10921 agcagcagga gcgcggcgct ggggacgagc acgaccccgg ccgaggtggg cgacacggcg
    10981 aagaacacga cgccgaccgc accgatgacg gtgaccgaca ccccggttcg cagcagtccg
    11041 acgacgccga agctgctcag catctctcgc tcggtgagcg gtgtggcgat caggttgagc
    11101 aggttgcggc tccacacctc gtcgagcagc ccgaaggagc cggccagggt gagctgccag
    11161 atcaggtgga acagcatgat ccccgtgacc agcaggacca ccggtgcgtc cccgatcccg
    11221 taggccacgc cgatcgaccc gaacaggagc ccgtcgacca gcggcagcac gagcagcagg
    11281 aaccagaagt cggggttgcg gctctgcgac aggaggctgc ggcgcaccat ggcccggacc
    11341 cgcctggtgt cgaacgtcgg tggggcgctc cgggaatcgg tctcggtcgt cgagggggtg
    11401 gcgggatcca tcagacctcc tgttcgcgca cgcgggcggc ctcggccagg aacatcgatt
    11461 cgagatcggc gtgaccgtgg gaagccacga cctggtccgg tgggccgtcc gcgatgatcc
    11521 gaccctggcg gaggaagatc acccgggtgg tcagctcggc gacctcgcgc atgtcgtggc
    11581 tggtcagcag cagcgtggcg ttgcgctccg agttcatgtg ctcgaggcgg tcccgcaccc
    11641 tcatcgccac gtcggggtcg agcgacgcgg tgggctcgtc caacacgatg aggcgcggat
    11701 ggtggatcac cgccttggcg aagccgacca gggtgccctg gcccgacgaa agcgactcca
    11761 ccttctggcc cgccagtcgg tcgatgtgca gctcgtcgag cacctcggcg gcccgggtgg
    11821 tcgggtcggc cacgccgtag agacggccga acagctcgag ggtctcggtg acggtgaggc
    11881 ccgacggcag cggaaggtag cccgccgcga agccgacccg ggtcatggcg tcgctgcgtc
    11941 ccccgggcag gcggtgcccg gcgatgtcga tcgtgccgtg gtcgggctcg atggcgccca
    12001 gcagcatgag cagcgtcgtc gtcttgcccg caccgttggg tccgacgatg ccgacccgct
    12061 ccccggcgtg gatcgtgatg ctgacgtcgt cgacggctcg ggtgtcgccg aagtccttcc
    12121 gtagtcgaga tgcccggagt acgacgtcag gtccggtcgg cgtcgttggc tgctccatcg
    12181 acccagtctc caccgccaca ccccgttctg tagtgcgaga tacaggcaaa cgcctggatc
    12241 tcgcacgaca gaacggtggg cgggagaatt ctcggcgatc ggtgtcgatc cacgtcgtcc
    12301 cgttcgtcgt ggtggtgacc gcaacgctcc ggaggttccg ggccggtcca ggacgaggag
    12361 aacgaccatg gcgcagtacc tgctctcggt gtggcacgac gaggactaca tcgacgcgtt
    12421 cgagaccacc ccgcccgagg tgatcgagcg gctccacgcc caagtgggag cgctcaacgc
    12481 cgacatggag aagaagggcg tctgggtgtt cggcggcggg ctggtcccgg cgtcgtcggc
    12541 caccgtggtg cgccccggca ccgacggcga cgtgtcgatc accgacgggc cctacgtcga
    12601 ggccaaggag atgatgggtg gcttctgggt gattgaagcc cccgacctgg attcggcgct
    12661 ggagtggacc cgccgggcgg cactcgcgtg cgagggagcc gtcgagctgc gcccgttcca
    12721 ggatgagtga gagcggagct cgggccgctc ggtccgcgag ctccgctggc cccgccgcgg
    12781 agctgagcaa tgcggtcgag tcggtgttcc ggcgggagtc gggacgctgc accgccacgc
    12841 tgatccgtgt cctcggcgac gtcgacctcg ccgaggacgc ggtggccgag gcgttcgagg
    12901 tggccctgac gcgatggccc cgtgacggcg tgcccgacaa cccgggggcg tggatcacca
    12961 ccaccgcccg gcggcgggct ctcgaccgac tccgacgtga gtcgacccgc cacgaccgac
    13021 acgtggccgc ccaccgcacg ttcgggaccg gcgacccgac cggcgctgac ccctcgaccg
    13081 tcggtcggct ggtcgaggaa ctgggaggtg agcaggtgga ggtcgttgcc gacgaccagc
    13141 tccgactgat gttcctgtgc tgccatccgg tgctggcgcc cgattcgcag gtggcgctga
    13201 cgttgcggct gctgggaggt ctgcagacgc cggagatcgc ccgggcgttc ctggtccccg
    13261 aggccacgat ggcccagcgg atcgtgcggg ccaagcgcaa gctgcgcgac aaccacgccc
    13321 ggtaccggat cccggtcgag gccgagctcc ccgaccggct gccgcccgtt ctcgcggcga
    13381 tctcgctgat cttcaccgag gggcaccgcg ccaccgacgg gtccggtctg gtccgcgtcg
    13441 agctgtcgaa cgaggcgatc cgactggggc gagtcctggc ggatctcatg cccgacgagc
    13501 ccgaggcggc aggcctgctg gcgctgatgc tgctgaccga cgcccgccgc tcggcgcgtc
    13561 tggatgcggc gggtgctctg gttccgctgg ccgagcagga tcgggaccgg tgggatcagg
    13621 atcggatcgc cgagggcctg gaccggctcg ccccctggag tcggcgggtg gatcccggtc
    13681 ggttccggat ccaggccgag ctggcggcgg ctcacgcgag agccgccacc ttcgccgaca
    13741 ccgactgggc gcggatcgtc gagctgtacg acgtcctgct gcagcggcgg tccgacccgg
    13801 tcgtggcgat caaccgggcc gtcgcgctcg gcgagctcga tgggcccgcg gccgcgctcc
    13861 tcgccctcga ggggctgacg accacgagcg tcagcagctc gactcctacc agccgtggca
    13921 cgccgcccgg gccgatctcc tggcccgggc cgggcgtccg tccgaggccg tcgaggcgta
    13981 cgaccgggcg atcgccctca ccgcctcgga ggcggagcgg cggttcctca ccgctcgtcg
    14041 ccaggcgtgc ggagccgggc agaccccgcc gtagccgtcc ggaggtcggc tgcggtgtag
    14101 acatcggcag gggcgcaccg acagggggat cgacatgacc gacacggccg cacggaacga
    14161 cacggccaca cggaacgaca cggccacacg gaacgtcacg gccgaccgcg aggaccgacc
    14221 gccgccgccg gaccgggcgg atccgaccgc gatcggcgcc cagcgggtcg ctctgccggg
    14281 cagcgacgtg aaggtcggcc ctctcggcca cctggcggcc gaacgccagc ggctcgacgc
    14341 cctgcgctgg gagcacttcg acgtcacccg gacctcggcg acgttggggg ccgaggtgag
    14401 cgggatctcc ctggccgacg acctgcccga tccggtcatc gccgacctgg cccgcgccct
    14461 ggccgactac aaggtgctgt tcttccggga ccagccgctc accacggccc agcacgtcgc
    14521 gttcgcccgc cggttcggtg agctcgaggt ccacccgttc ctgccggcga acaccgacag
    14581 ccccgaggtc gtccgcttcg ccaagtccgc ggacgtcggt ggctacgaga acggctggca
    14641 ccacgacgtc acctggcgcg aggtgccatc gatgggggcg gtgctccacg ccgtcgaggt
    14701 gcccgactcc ggaggggaca cgctgttcgc ggacgcccac gccgcgctcg acgagctcga
    14761 gccgcagctg cgcgagcgtg tcgacgggct gctggcggtg cacgacttcg tccacgtgtt
    14821 cggccacacg ctgtcgccgg acgacctcgc ctccatgcgg gagctgcatc cgccggtggc
    14881 gcatccggtg atcgccaccc acgaccgcac tggccgggac cacctgtacg tgaaccggtt
    14941 cttcgtgtcc cacctgaccg aggccgacgg tgagcccctc gacccggccg agagcgccca
    15001 gctcatcgac gccctgtgcc gaccgttcga gacccccgag ctgcaggtga ggctccgctg
    15061 ggagccggac tcggtcgcgt tctgggacaa ccgggcggtg cagcactacg cctgcagcga
    15121 ctactggccc gacgtccggg tgatggagcg ggccagcatc gtcggcaccc gacccgtgcg
    15181 acgctgatcg acccgcaccc acccccgctt tgtcccgcac agacgcggtg atcaccgcgt
    15241 ctgtgcggga caaagcggtg gcgggacaga gcgggggcgg tagcgtccgg cccccgtggg
    15301 gagctacacg gcctcggcgg tcgcagcgat cgtcggtggc gtcatcgtcg ccgtcgtcat
    15361 cttcgtgccc gacgtggcgt accggtaccg ccggtccggg aggctgcggg gatcggacgt
    15421 ggtggccgtc gtcgtggtcc cgacctacgc gctggcgctg tggacctaca ccctgctgcc
    15481 gttgccggac cccgacgaca tcgtgtgtcg cgacccgaac ctcgacgcct tccgcttcgt
    15541 cgatcagatc cgggcgtcgg gggtggactc tctggcggac ctgctgacga actgggcgtt
    15601 cgcgtcgacg gtgctgaacg tcctgctgtt cgtgccgctg ggggtcatcc tccgttggcg
    15661 gttccggcgg ggagtgctgg tcgccctggt cgtcggcttc gccgtgtcgt tgctcatcga
    15721 gacgacgcag ctgaccgggc tgtggggggt ctacgactgc gcgtaccgct acttcgacgt
    15781 cgatgacctg atcaccaaca cgaccggcgc cgtcgtcggg tcggtgctgt cgtggctggt
    15841 gctgcgccac cggcccgatc cggaggtcgt ccccgacgag gccatggcga cctcgggtca
    15901 gcggatcgtc gccgtcgcct gcgacgtcgt ggtcatgctg ctggtcggca cggccaccgt
    15961 cgtcgcctgg cgggcgatcg cgctgcggac cctgaagatc gacgaggccg tccacccgct
    16021 ggtgcagcga tcgctgcagt ggggcgtgcc gctggcgctc gaggcgatcg ccgtcgtggg
    16081 cttcggacgc acgatcgggg agtgggcggt gcgactcggg acggtcgccc ggcgacccgg
    16141 ctggacgctg cccgggcgcg tgctcaagct ggcgttcggt ccggggctga tcgcggcggt
    16201 cgggtggtgg gactcggggt ggtccggcgt gctgctgttc gtcctggccg ccctcagcgt
    16261 ggtggtggcg gtggtgacgc cggaccatcg ggggctggcc aacaccgttt cgggtctgcg
    16321 cccggtcgtc acgccgtggg gtcgtccagc cgatcccgac tgatctccat cggtcgtacc
    16381 gtggggccgt ggccccgacg ttcctgaccg agttcgaccc gcggtacggc gagccggtgg
    16441 cggtctccga cggggtcgtc cgtgtggtgg cggacaaccc gagcaagtac accgcgtggg
    16501 gaacggggac ctacgtggtc ggcgaggccc aggtggccgt catcgacccc ggcccggacc
    16561 tgcccgccca cgtcgacgcc ctgctcgcgg cgctcgacgg tcgagcggtg tcgcacatcc
    16621 tgatcaccca cacccacgcg gaccactcgc ccgcggccgc cgcgctggcg gcggcgacgg
    16681 gagctcccac ctacgggttc gggccccatc cgatcgatgc cggaaccggc gacgacgcgg
    16741 tcgacgggcc ggatgcggca gaccggcctg atgctgcaga ccggcctgac gcagccgacg
    16801 ggggcgagga gaccgagtcg ggagatcggg acttcgtccc cgacgtcgtg gtgcgcgacg
    16861 gcgatgtgct ggtcggcgac gggttccgct tcgagtgcct ccacaccccc ggccacatct
    16921 ccaaccacgt gtgcttcgcc gaggtggagc gcaacctgct gttccccggc gaccacgtga
    16981 tgggctggtc caccacggtc gtgtcgccgc ccgacggcga catggacgcc tacatcgcca
    17041 acctccgtcg cctggtcgac cgacacgccg caggccggga cgccatctac ctgcccaccc
    17101 acgggtcggc catcgtggac ccgggtccct tcgtcgagtc cctcatcgag caccgactcg
    17161 aacgcgaccg ccagatcctc gacctgctcg ccgcagctcc cgccggtgac ggcgtcagtg
    17221 tcgagggtct ggtggcggtg atgtacgcgg acgtccgtcc ggagctgcac cggccggcgg
    17281 gtcgctcggt gctcgcccac ctgatcggcc tggccggccg ggggtcggtg gagcgccggc
    17341 ccgatggtcg gtggcagctc cggcgacgac cggggcagtc tggggcgatg accgtcacgg
    17401 gaccggtgct cccctgagca ccccgaacga accacgtccc gtgaccggac cgggctccgc
    17461 aactccaccg ggttccgcag ctccgccggc cgccgcggtc ggaccgagcc cgtcgcccgg
    17521 aacgtcggcg gagcccccga ccggaccgga cgtccgcaac gggatctccc tggccgtcgc
    17581 gtcggccgca tgcttcggct tgtccggatc gctggcctcg gcgctgatgc tcagcgggtg
    17641 gaccgcgggc gccgcggtga ccgcccgggt catgatcgcc gcgctggtgc tggtggtccc
    17701 cggcgtacga gcggcgggtg tcgacggtcg ggaccgctgg gcggtgctgc ggcgcaacgc
    17761 ggggacgatc gtggcctacg gggtgctcgc ggtcgccgcg acccagctct gctacttcta
    17821 cgcggtgtcc cacctgccgg tcggggtcgc actgctggtc gagtacaccg ccccggtcgg
    17881 agtcgtcggc tggatgtggg cggtgcaccg tcaacgcccg agccggctga cggtgctcgg
    17941 cgcagcgttc gcgctgtgcg ggctgccgct gctgctcgac ctgctgggtt cggcgggcag
    18001 cggaggtggg gtggaccccg tcggggtcgc ctgggcgctc gccgcgatgg tcggcgccgc
    18061 ggcctacttc gtcatgtccg ccgatgaccg gtcgggtctc ccgccgatca cgctggccgc
    18121 atcgggcctg gtcgtcggcg cggtcgtcct gggcgcggcc gggctgctgg gcgtgctgcc
    18181 ggtcaccgcg acgaccgacg acgtcgcgtt ccagcggctg gtcgtcccgt ggtgggtccc
    18241 ggtcctggcg ctcggggtgg tcaccgccgc agtcgcgtac gtgaccggga tcgccgccac
    18301 ccgacggctg ggttctcgcc tggccagctt cgtggcgttg accgaggtga tcgcggccac
    18361 cgcgttcgcg tggctgctgc tgggccagac gccgttgccg gtccagctgg tcggtgccgc
    18421 ggtcgttctc ggcggcgttg ccgtggtccg cctcggcgag ccctgaccgt cgcgacccct
    18481 gccgcagcgg ttctgtcgtg cgagatccag gcgaacgcct ggatctcgca ctacagaacg
    18541 gggagggggc aggaacgggt ggggctactt gatcaggggg tcgcggggga ggccgaggat
    18601 ccgctcgccg atctggttcc gcttgatctc cgaggtgccg ccggcgatcg acatgccccg
    18661 gtgccacagc accagcgccc ccgacgtgag cccggcggca tcgaggtagg cggcgtcggg
    18721 gttggtcgcg ttgagcaccg cggccgcctc ggccaggttc tccgacagca ccagcttggt
    18781 gatcgcacct tcagggcccg gtgcggcgcc ggccacggcc c
//
LOCUS       DAOQOD010000048        18403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1064_length_18403_cov_11.125027, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000048 DAOQOD010000000
VERSION     DAOQOD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 18403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18403
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_18403_cov_11.125027"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLZ72_06625"
     CDS             complement(<1..317)
                     /locus_tag="PLZ72_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNA36007.1"
                     /translation="MADHNPLGVMPSMADDLARVEAELGDAVRSGDEYLTEISSHLIA
                     AGGKRVRPGFCIASAATALTVDAPASAAAVTGGVAVELVHLGSLYHDDVMDEATMRRT
                     VESV"
     gene            506..3034
                     /locus_tag="PLZ72_06630"
     CDS             506..3034
                     /locus_tag="PLZ72_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNA36008.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEEIIGQGGSSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLSRVRQQVIQLLSGYPGSSTQPPGGGEKAG
                     ATSGGSGGETPSGSLVLDQFGRNFTQLARDKKLDPVIGRDRETERMMQVLSRRTKNNP
                     VLVGEPGVGKTAIVEGLAQAIAADQVPETLKDKQLYTLDLGALVAGSRYRGDFEERLK
                     KVLKEIKTRGDIILFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEY
                     RKHLEKDAALERRFQKVVVDEPSVAHTIEILKGLRERYEQHHRVTITDQALVAAANLA
                     DRYISDRFLPDKAIDLIDEAGSRLRIRRMQTPPDYREIESELADVVRQKKEAVESQDF
                     EAAGGLRDREKELLAKKEEKDLEMKAAGVDLFDEVDEEAIAEVLSLWTGIPVYKLTEE
                     ETAKLLRMEDELHKRVIGQEDAIKAVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKT
                     ELAKTLAEFLFGDDQAMISLDMSEYMEKHTVSRLVGSPPGYVGYEEGGQLTEAVRRKP
                     FSVVLFDEIEKAHPDVFNTLLQILEEGRLTDSQGRSVDFRNTVLIMTSNLGTADLRKA
                     NVGFSKRDEAVTYERMKEKVNEALKAHFRPEFLNRIDDVIVFHELSQDEVIQIVDLMI
                     ERVTTQMAAQGMGLEVTPAAKVFLGERGYDPTLGARPLRRAIQRLVEDPLSERLLWKE
                     FRAGQTIVVDVEPDPDDDGQLHAVFRAVEGFTPPPIEELAATGD"
     gene            3306..3674
                     /locus_tag="PLZ72_06635"
     CDS             3306..3674
                     /locus_tag="PLZ72_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36009.1"
                     /translation="MALLVGAGVACAPGTPQAVLNVPGNYVANLWNPPPGYQQGAVAS
                     VIMCLDSTSAVRLETGDGGQGSVGVIRLEWAGSTYAPVGTGSAITLTTPVLSPGCGTL
                     TFGPDCCHVDNYLAIKATKV"
     gene            3798..4583
                     /locus_tag="PLZ72_06640"
     CDS             3798..4583
                     /locus_tag="PLZ72_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36010.1"
                     /translation="MPALSRSLSPGARTRSPWVLVLGILVALTAAACRTDVDVLVAVD
                     PDGSGKVTVTADLDADAAAQLGDPSRLVLTDLADAGWDVDGPTAWRGGLRVVAVRRFS
                     SPEELSAVLDEVGGAGGVFSDTSLVLGNGLLSDSTEFRTRLHLTGDPAQFSDEQLAQV
                     LGGLPLGRTPEELAAEGFDSADAATLTVRVALPDGVDESNGRITKGVARWSAPLAGGT
                     STDTDLRASASTLRTSTLVLVALGAVLLVGAAVVAVIGAARRD"
     gene            4659..6014
                     /gene="radA"
                     /locus_tag="PLZ72_06645"
     CDS             4659..6014
                     /gene="radA"
                     /locus_tag="PLZ72_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNA36011.1"
                     /translation="MARTRTVFRCMSCGAGAPKWSGRCSSCGDWNTLSEELETPDAGL
                     EPLAASSPPTPITQLTAADGAPVPTGIAELDRVLGGGFVPGSVTLLGGEPGVGKSTLV
                     LQALAARARAGARVLYVTGEESADQVRLRAERIDALDDNLLLAAETAMGNVAVHLEAV
                     RPELVVVDSVQTLFDARFSSAPGSVTQVRECAHRLVQIAKATGTSVLLVGHVTKDGQL
                     SGPRVLEHVVDTVLSLDGDRHHDLRILRAVKHRFGSTDEIGMFSVHGDGLASMADPSS
                     LLLGDRTPGLPGSVVVPALEGQRPLLVEVQSLVAGSSLAQPRRSAQGLDQGRLSLLLA
                     VLQQRVGVDLSQADVYAMTVGGVKVTEPAADLAVCLAAVSSATGAPISPGVVACGEVG
                     LGGELRQVRRIERRLAEAARLGFTTAVVPHSTPTIAVPMEVLRVPSLRVALDTVGLLE
                     Q"
     gene            6123..>7092
                     /gene="disA"
                     /locus_tag="PLZ72_06650"
     CDS             6123..>7092
                     /gene="disA"
                     /locus_tag="PLZ72_06650"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNA36012.1"
                     /translation="MALRRNPELQAALAAVAPGQPLREGLDRILKAGMGALIVIGDGP
                     EVLNISSGGFLVNAAFSPQRLSELAKTDGAIVLTPDAGYIARANVHLVPNPNVPTEET
                     GTRHRTAERVARSIGVPVLSVSEDMAVVTVYTKSTRYQLEPITAILNRCNQALQTLER
                     YKGRLNEESTNLTSLEVEDLVTLRDVVALLQRAELVARISDEIERHLVELGTDGRLVR
                     LQLRELMAGVEDERRLVVLDYLQTDASWDLEQAIDTLSDLDMEDLLDPDKVAHALHLS
                     GHGQEDDMDAHLQPRGYRMLARIPRLPDELADRLVAHYGSLGKLSRA"
     assembly_gap    7093..7192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7427..8092
                     /locus_tag="PLZ72_06655"
     CDS             7427..8092
                     /locus_tag="PLZ72_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNA36013.1"
                     /translation="METLPSGTPAHLARSERASRGLIVIPDIWGLRPLFTEMCDDLAA
                     RTGWSVGTFEPFPGRDLPNEGEPDAGPARFAVLPELSDDRLLGDAVDLADLTGCDDVG
                     LIGFCMGGMYALKAAATGRFDRIAAFYGMIRVPEAWQGPGQGDPLDALDARGSTEVMA
                     VVGSVDPYTPTDHVQELRDRGVDVVHYEGADHGFVHDPTRPSHRADDAADAWDRVLTF
                     LAG"
     gene            complement(8119..8769)
                     /locus_tag="PLZ72_06660"
     CDS             complement(8119..8769)
                     /locus_tag="PLZ72_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNA36014.1"
                     /translation="MDLLHNVELFDRFDDEDHRRVADAAERVDLIRSDVVFTEGAEAD
                     ACYVVIDGRVAIANKSIDGRESMVALMERGDLFGEMGFFDGMGRSAEARALEQSSVIR
                     IPYDVLQALWEDKPELLWSVVRMLSQRIRATDEALADAFFLDVTGRTAKHLLELAGDR
                     DDFEIPITQEELAGLVGASRERVNKAIASFLRLGWIEQNDRQYRILKRRELEIRST"
     gene            8898..9590
                     /gene="ispD"
                     /locus_tag="PLZ72_06665"
     CDS             8898..9590
                     /gene="ispD"
                     /locus_tag="PLZ72_06665"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNA36015.1"
                     /translation="MSEVATGPVWCVVVAAGRGSRFGGPKQQAIRAGRTVLERSVATA
                     GAACDGVVVVVPDDWTDPVDGADRVVVGGATRSASVASGLDAVPADAAVVLVHDAARP
                     LASPELYRRVIEAVRSGADAVVPVVAVIDTIRSLDGGTVDRDLLRAVQTPQGFRVEAL
                     RAAHRSGGDATDDATLVEAVGGSVELVDGERTNLKITEPSDLDVAEALLRRAAASTGG
                     DTDGAPGGDSLL"
     gene            9587..10090
                     /gene="ispF"
                     /locus_tag="PLZ72_06670"
     CDS             9587..10090
                     /gene="ispF"
                     /locus_tag="PLZ72_06670"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNA36016.1"
                     /translation="MSIRVGNGFDIHRFSDAPDRVLVLGGVSFPGEPGLHGHSDADVV
                     AHAVAEALLGAAGLGDLGSHFPDTDERWRGADSLELLAEVVRLLHEDGWSAVNVDCSV
                     IAERPKLAPHRDRMQVVLSDLVGAPVTVKGRRAEGIGGLGRAEGIACLATALIETRVH
                     FVDQRSS"
     gene            10090..11139
                     /gene="rlmB"
                     /locus_tag="PLZ72_06675"
     CDS             10090..11139
                     /gene="rlmB"
                     /locus_tag="PLZ72_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNA36017.1"
                     /translation="MAGRSPGGSRRGGSDRSRSSGSGSAGRGGKGSGASGRASGGSRG
                     GGGPKGAGPRRSGGARGGAPSGRANPPGSERARQGAAARRAPRKDPGRTTAPKERGLG
                     GTQVEGRQAVRELLLAGRRKVYEVFLNAEMDRADIVGDIVELADELRVPITEVSRGRL
                     DSISHTEAPQGVVARAAELPHTALHDLCEPVDGRPPFLLAVDGVTDPGNLGALLRIAE
                     CAGVTGVVLPRHRAVHITPTVTKAAAGAVEYLRMALVGGLPTAISDLRDAGVWVVGLD
                     AGGEVSVHDLPVATDAVCLVLGAEGKGLSRLAAQRCDLVASIPLHGHLSSLNVASAGA
                     VACYEIARRRPPATV"
     gene            11165..11926
                     /locus_tag="PLZ72_06680"
     CDS             11165..11926
                     /locus_tag="PLZ72_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36018.1"
                     /translation="MSDSTTTDPRPAALDAARHTAQAVQDVGAKFMLDMDMYGEVAGL
                     GYQGLGFYIAGRGGVLGDVEHVEVFEAMTFFPAETVQAGWESSASVESRADSAGRFAG
                     YAARWGANNVADGTEGLDRLAELCEKVIQAADGSDAPLFAGWRDLPEPDGEPELVVHR
                     MNALRELRAARHIAAIRQVGLDPATAFMIRTPYMAGIFGWPEPSEPPAEIDKDLWQSA
                     EDLTERAFAADLAVLDDDELAEFCRLTDALLAAIP"
     gene            complement(11975..13057)
                     /locus_tag="PLZ72_06685"
     CDS             complement(11975..13057)
                     /locus_tag="PLZ72_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNA36019.1"
                     /translation="MTVRSPEISPAHRRVIKRGIRIGVAAGLATLCSQLLHLPNPWFA
                     TVAAIVAIQGTLQATFRSGRNSLVGAFIGAALGLGVAYVAKDQAWAVALVVAAPLVLF
                     GWFRLPAIGQQAALVASVVVLVPESTDFSTFDFALIRLSQAVIGIVVAMAVQAVLFPP
                     RAHRTARRELGGVYRDMAHLLGSATEVLDGQPRRADAVRQARIDGRARLAKVDDLWDD
                     AMAEHPSHGLLGSHWRVTTRRIWEQCAVLSTEVADAGDSALLRACRDELTTLTLALQQ
                     AMHDISGWFRHAHPDSPIELTNLEPVRLAALNRVRRVEQGDRADPYPRTLQALAVANA
                     CNVIAERLTDLDTQHRDAVRVHGPAE"
     gene            complement(13099..13791)
                     /locus_tag="PLZ72_06690"
     CDS             complement(13099..13791)
                     /locus_tag="PLZ72_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HNA36020.1"
                     /translation="MTTTPDTTKTPDTTTTPDTTTAPGSTSMEPLARLSAAVGVAATT
                     IDALDPAQYDRPTPCPPMSVGALAEHLVMALRRVALAGRDVPLAEWPIDAADVAAGTW
                     GDALRVAGSDVEQAWADPALLERPTTLPWGTFPGREVLDVYTNEMVIHTWDLATATDQ
                     HPEWDPAVLEASLAVMHAQLPIPVRAPIWEAIYAGMPAEVPRDIPFADAVDVAEDAPL
                     IDRLVAWNGRTP"
     gene            13897..>14772
                     /locus_tag="PLZ72_06695"
     CDS             13897..>14772
                     /locus_tag="PLZ72_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA36021.1"
                     /translation="MRADRLVAILLMLQRRDVVTAAEVAEELEISVRTARRDLEALAM
                     AGVPVYPQRGRGGGWRLLGGGRLDLSGLTAGEARSLFVLVGGRPEVGPEVRSALRKLV
                     RALPEPLREGAEAAAGSVVVDPVGWDRHRGEPRRPDRLDDLEAAVIDGECLHLGYRAR
                     SGEVTERVVHPLGLAAKGRNWYLVAGTATGLRTFRADRVERAERTGEPVVRPDGFDLS
                     EAWARIVSDVDQLRTPLEALGTAVPEALPYLRMVLGRRVEVIGDEAAGSDPAGSDLAG
                     SGPESATPSDGSVRIR"
     assembly_gap    14773..14872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14873..15178
                     /locus_tag="PLZ72_06700"
     CDS             <14873..15178
                     /locus_tag="PLZ72_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HNA36022.1"
                     /translation="ADARGALVSLSAVRHLDGGEQRFRWSAHLDDVDPDSVTASLADG
                     VLTVSAATRPGPVARTVEVTVADPSEPAALPADTPSDEVGSESAEPTVTAEDTSEEV"
     gene            complement(15283..15936)
                     /locus_tag="PLZ72_06705"
     CDS             complement(15283..15936)
                     /locus_tag="PLZ72_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HNA36023.1"
                     /translation="MTTDPPRRPRLSRERVLDAAVAIADERGLAALTIRSLADRLGSR
                     PMALYHHVRNKEEILDGIVDVVFSEIDLPRIGGDWRAEMRRRADSARAVLHRHPWSIG
                     LLESRAAPGPAILRHHDAVIGTLRVAGFSIADTAHVYAVIDSFVYGFALQEAGLPFDG
                     PDDIAGFAEGVLDHMPVDEYPWLVEMTTEHVMQPGYDFGDEFAVGLELILDSFAPDE"
     gene            16047..17099
                     /locus_tag="PLZ72_06710"
     CDS             16047..17099
                     /locus_tag="PLZ72_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNA36024.1"
                     /translation="MRSDRELHTPTRPDPGSTMRAVVQSSYGPPDQLRIETLGVPQVG
                     DTDVLVEVHAAGAGRGAWHLLTGTPLMARLGIGLRRPRRPVAGTDVAGVVLATGAKVD
                     RFEPGDRVFGIGRGTIAEMAVVPASKLASIPPNCSFAEASILAESGLTALQAVHDRAE
                     VRPGDRVLVLGASGGVGSFAVQMSAGLGARVTGVCRSDKTSAVRAFGADEVVAYDRAD
                     ITERGDRELFDVIVDAGGRHPLRRLRRILTPRGRLVIVGGEGGGRITGGFGRGFRAAA
                     LSPFVGQRLTMVVSKETSADLERLGELVADGTVRPPLERTLPLGRAADAFALLEAGEV
                     TGKVAVTVCAPSGDDG"
     gene            complement(17260..17862)
                     /locus_tag="PLZ72_06715"
     CDS             complement(17260..17862)
                     /locus_tag="PLZ72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF028039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HNA36025.1"
                     /translation="MARNVLIALGAFGVLLVGGCGVLLVAGGAAVDKAAESIDKEIAS
                     STSTTAESVAPAEPSTASGSESSFVDGVLTTPKMIIKITDKKVIPVGEPGNEYGDKPV
                     IAFWYETTNLTDGDVSPMNWVFVFSAYQDNDPNAVNELDLASLPDPQFLENQMVKIKK
                     GGTVASAVAYELDDQTTPVELVASEGLGSHEIGRMTYNFG"
BASE COUNT         2588 a         6382 c         6551 g         2682 t
ORIGIN      
        1 acgctctcga cggtgcggcg catggtggcc tcgtccatga cgtcgtcgtg gtagagcgac
       61 cccaggtgca ccagctccac cgcgacgccc ccggtcaccg ccgccgcgct cgccggcgcg
      121 tccacggtca gggccgtggc ggccgacgcg atgcagaagc cgggtcgcac gcgcttgccg
      181 ccggccgcga tcaggtgcga cgagatctcg gtgaggtact cgtctccgga tcggaccgcg
      241 tcgccgagct cggcctcgac ccgggccagg tcatcggcca tggagggcat gaccccgagc
      301 gggttgtggt cggccatctg cgccacgcta cccggcgccg gcagggcgga ccccaccggc
      361 cgatgtcaca cgtggctctc cgtcgggcgg ctcttccggg acgatcgtcg ggcgttcggg
      421 ggagtcccgg agcctccaca gggcggggaa cgggtgacgg gggtcgggcg ttacacttac
      481 ccaacgtctt ctcgggaggc actaagtgtt cgaacgtttc accgaccggg cccgtcgggt
      541 ggtcgtgttg gcccaggagg aggctcgcct cctcaaccac aactacatcg ggaccgagca
      601 catcctcctc ggcctcatcc acgagggtga gggagtcgcg gccaaggcgc tcgagtcgct
      661 cggcatctcg ctcgaggcgg tgcgccagca ggtcgaggag atcatcggcc agggcggatc
      721 gtcgccgtcg ggtcacatcc cgttcactcc ccgcgccaag aaggtcctcg agctgtcgct
      781 ccgcgaggct ctgcagctcg gtcacaacta catcggtacc gagcacatcc tcctcggcct
      841 catccgcgag ggcgagggcg tggccgccca ggtgctggtc aagctcggcg cggacctgtc
      901 ccgggtgcgt cagcaggtca tccagctgct gtcgggctac cccggatcgt ccacccagcc
      961 cccgggcggt ggagagaagg cgggtgccac ctccggtggg tcgggtggtg agacgccgtc
     1021 ggggtcgctg gtgctcgacc agttcggtcg gaacttcacg cagctcgcgc gcgacaagaa
     1081 gctcgacccg gtgatcggtc gcgaccgcga gaccgagcgg atgatgcagg tgctcagccg
     1141 ccgcaccaag aacaacccgg tgctggtcgg cgaacccggc gtcggcaaga ccgccatcgt
     1201 cgagggcctc gcccaggcga tcgccgcgga ccaggtcccc gagacgctga aggacaagca
     1261 gctctacacg ctcgacctgg gtgcgctcgt cgccgggtcc cgctaccggg gtgacttcga
     1321 ggagcggctc aagaaggtcc tcaaggagat caagacccgc ggcgacatca tcctgttcat
     1381 cgacgagatc cacaccctcg tcggcgcggg tgccgccgag ggcgcgatcg acgccgccag
     1441 catcctcaag cccatgctcg cccgcggcga gctccagacg atcggcgcca ccaccctcga
     1501 cgagtaccgc aagcacctgg agaaggacgc cgcgctcgaa cgccgcttcc agaaggtggt
     1561 cgtcgacgag ccgtcggtcg cccacacgat cgagatcctc aagggcctgc gtgagcgcta
     1621 cgagcagcac caccgggtga cgatcaccga tcaggcgctg gtcgcggcgg ccaacctggc
     1681 cgaccggtac atctcggacc gcttcctgcc cgacaaggcg atcgacctga tcgacgaggc
     1741 gggctcacgt ctgcgcatcc gccgcatgca gacgccgccg gactaccgcg agatcgaatc
     1801 cgagctcgcc gacgtcgtcc gccagaagaa ggaagcggtc gagtcccagg acttcgaggc
     1861 cgccggtggc ctccgcgacc gggagaagga gctcctcgcc aagaaggagg agaaggacct
     1921 cgagatgaag gcggccggtg tcgacctgtt cgacgaggtc gacgaagagg cgatcgccga
     1981 ggtcctgtcg ctgtggaccg gcatcccggt ctacaagctg accgaggagg agaccgccaa
     2041 gctgctgcgc atggaggacg agctgcacaa gcgggtcatc ggccaggagg acgccatcaa
     2101 ggcggtcagc caggcgatcc gccgcacccg ggccggcctc aaggacccca agcgcccgtc
     2161 gggctcgttc atcttcctgg gcccctccgg cgtcggcaag accgagctgg ccaagaccct
     2221 cgccgagttc ctcttcggcg acgaccaggc gatgatcagc ctcgacatgt cggagtacat
     2281 ggagaagcac accgtcagcc gcctggtggg ctcgcctccc ggctacgtcg gctacgagga
     2341 gggcggtcag ctcaccgaag ccgtccgccg caagccgttc tcggtcgtcc tcttcgacga
     2401 gatcgagaag gcgcaccccg acgtgttcaa cacgctgctg cagatcctcg aggagggtcg
     2461 cctgaccgac tcgcagggtc ggtcggtcga cttccgcaac accgtgctca tcatgacgtc
     2521 caacctggga acggcggacc tgcgcaaggc caacgtcggc ttctccaagc gcgacgaggc
     2581 ggtcacctac gagcgcatga aggagaaggt caacgaggcg ctcaaggcgc acttccggcc
     2641 cgagttcctg aaccgcatcg acgacgtcat cgtcttccac gagctgagcc aggacgaggt
     2701 catccagatc gtcgacctga tgatcgagcg ggtgaccacc cagatggccg cccagggcat
     2761 gggtctcgag gtcacgcctg cggccaaggt cttcctgggc gagcggggct acgacccgac
     2821 gctcggcgcc cggccgctgc gtcgggcgat ccagcggctg gtcgaggacc cgctgtcgga
     2881 gcgactgctg tggaaggagt tccgcgccgg ccagacgatc gtggtcgacg tcgagcccga
     2941 ccctgacgac gacgggcagc tccacgctgt gttccgcgcg gtggagggct tcaccccgcc
     3001 tccgatcgag gagctcgccg ccaccggcga ctgagcccgg accggaccga cttgtttcgt
     3061 tctggctgtc gtcctgccgg tcaatccggc aggacgacag ccagaaccgc gttcgggacc
     3121 tccggcccta ccggcatctc ccgacgtgtg gtagtccacg accggagggc gcggtcgggg
     3181 accagaccgc cgtggagtcc gtcgggcggt ctccgcagaa cggaggtgca ccaatgccca
     3241 cttcatccag atcgaaggcc cccgtccgga tcggccgggg acggaccgcg gcgatcgttg
     3301 cggtcgtggc cttgctcgtc ggtgccgggg tggcctgcgc tccggggaca ccgcaggcgg
     3361 tcctgaacgt cccgggcaac tacgtggcca acctgtggaa cccgccgccg ggctaccagc
     3421 agggtgccgt cgcgtcggtg atcatgtgcc tcgactcgac ctcggcggtg cgcctcgaga
     3481 ccggtgatgg tggtcagggc tcggtcggcg tcatccgact cgaatgggcc ggatcgacct
     3541 acgccccggt gggcaccggt agcgccatca ccctgaccac gccggtcctg tcgccggggt
     3601 gcggaacgct gacgttcgga cccgactgct gccacgtcga caactacctg gcgatcaagg
     3661 ccaccaaggt ctgatccggc cccttctcgg gcaccgatga ccgccggatg gtcgcaggtg
     3721 cccgagttcg atcggggcca ggtcgtcgat cgaccggccg ccatgggccg cgcgtcggcc
     3781 gggtagcgtt cgaagccgtg cccgccctgt cccgctcgtt gtctcccggc gctcggaccc
     3841 ggtcgccgtg ggtgctggtg ctgggcatcc tggtcgcgct gacggccgcg gcgtgtcgga
     3901 ccgacgtcga tgtgctcgtg gcggtcgatc cggacgggtc cggcaaggtc accgtcacgg
     3961 cggacctcga cgccgacgcc gcggcacagc tgggcgaccc ttcacgactg gtcctgaccg
     4021 acctggccga cgccggctgg gatgtcgacg ggccgacagc ctggaggggc gggctccggg
     4081 tcgtcgccgt gcgacgcttc tcgtcgccgg aggagctctc cgcggtcctc gacgaggtcg
     4141 gcggggcagg aggggtgttc tccgacacat cgctcgtcct cggcaacggc ctcctctcgg
     4201 actcgacgga gttccggacc cggctccacc tgaccgggga cccggcccag ttctccgacg
     4261 agcagctggc acaggtcctc ggaggcctcc cgctgggtcg cacccctgag gagctggcag
     4321 ccgagggttt cgactccgcc gatgccgcaa ccctgaccgt ccgggtcgcc ctgcccgacg
     4381 gggtcgacga gtccaacggt cgcatcacca agggggtggc gaggtggtcg gctccgctgg
     4441 ccggtggtac ctccaccgac acggacctgc gggcgtcggc ttcgacgctt cgaacctcga
     4501 cactggtgct ggtcgccctg ggggcggtac tgctcgtcgg cgctgcggtc gtcgcggtga
     4561 tcggggcagc ccgacgcgac tgacccgtcg ttcggtgtgg gaacacccgt tcgacccatc
     4621 cgcgtcggct gtcacccccg tcgcctaccg tcgggcccat ggcacggacc aggaccgtct
     4681 tccggtgcat gagctgcggt gcgggtgcgc ccaagtggtc ggggcgctgt tcgtcctgcg
     4741 gtgactggaa cacgctgtcc gaagagctgg agacgcccga cgccggtctc gaaccgctcg
     4801 ccgcgtccag tccgcccacc ccgatcaccc agctgaccgc cgccgacggc gcgccggtgc
     4861 ccacagggat cgccgagctc gaccgggttc tgggcggggg cttcgtcccc ggcagcgtca
     4921 cgctcctggg cggggagccc ggcgtcggca agtcgaccct ggtcctgcag gcgctcgcgg
     4981 cgcgggcgcg tgccggcgcc cgggtcctct acgtcaccgg tgaggagtcc gcggaccagg
     5041 tgcgcctgcg cgcggagcgg atcgacgccc tggacgacaa cctgctcctg gccgccgaga
     5101 ccgccatggg caacgtggcg gtgcacctcg aagcggtccg gcccgaactg gtggtggtcg
     5161 attcggtgca gaccctgttc gacgcccgct tctcgagcgc ccccggctcg gtcacacagg
     5221 tgcgcgagtg cgcgcaccgg ttggttcaga tcgccaaggc caccggcacg tcggtgctgc
     5281 tggtcggcca cgtcaccaag gacggccagc tctcggggcc ccgggtgctc gaacacgtgg
     5341 tggataccgt cctgtcgctc gacggcgacc gccaccacga cctgcgcatc ctgcgggcgg
     5401 tcaagcaccg cttcggctcc accgacgaga tcggcatgtt cagcgtccac ggcgacggcc
     5461 tggcgtccat ggccgacccg tcctcgctgc tgctcgggga ccgcaccccg gggttgcccg
     5521 ggtcggtggt cgtgcccgcg ctagagggtc agcggccgct gctggtcgag gtccagtcgc
     5581 tcgtcgccgg gtcgtcgctc gcccagccgc ggcggtcggc ccaggggctg gaccaggggc
     5641 gcttgtcgct gttgctcgcc gtgctccagc agcgggtcgg cgtcgacctg tcgcaggccg
     5701 acgtctacgc catgaccgtc ggaggggtga aggtcaccga accggccgcg gacctcgccg
     5761 tgtgcctcgc cgctgtgtcc tcggcgaccg gcgcccccat ctctcccggg gtcgtggcct
     5821 gcggagaggt cggcctgggc ggtgagctcc gacaggtgcg tcgcatcgaa cggcgactgg
     5881 ccgaagcggc ccggctcggc ttcaccaccg ccgtggtccc ccactccaca ccgaccatcg
     5941 cggtgcccat ggaggtgttg cgcgtgccca gcctccgcgt ggcgttggac accgtcggcc
     6001 tcctggagca gtgaatggac ggcccccacc cgacgccgga accgacgccg gggtggccga
     6061 acgaacgccg accgcggtgg actgaacgac cgatcaggcg acccgggcac taggctgcgt
     6121 cgatggcgct gcgccgcaac cccgaactgc aggccgcgct ggccgcggtc gcgccggggc
     6181 agccgctgcg agagggcttg gaccggattc tcaaggccgg catgggggcc ctgatcgtga
     6241 tcggcgacgg accggaggtg ctcaacatct cctccggcgg gttcctggtg aacgcggcgt
     6301 tctcgcccca gcggttgtcg gagctggcaa agaccgacgg tgccatcgtc ctcacccccg
     6361 acgccggcta catcgcccgc gccaacgtgc acctggtccc caacccgaac gtccccaccg
     6421 aggagaccgg gacccgtcac cggacggccg agcgggtggc gcgctcgatc ggcgtcccgg
     6481 tgctgtcggt gtccgaggac atggcggtgg tgaccgtcta caccaagtcg acccgatacc
     6541 agctggagcc gatcacggcg atcctgaacc gctgcaacca ggcgttgcag accctggagc
     6601 gctacaaggg gcgtctcaac gaggagtcca ccaacctgac ctcgctcgag gtcgaggacc
     6661 tggtcacgct ccgcgacgtg gtcgccctgc tgcagcgggc ggagctggtg gcccgcatct
     6721 ccgatgagat cgagcgtcac ctggtcgagc tcggcaccga cggccggctc gtgcgactgc
     6781 agctccggga gctgatggca ggggtggagg acgagcggcg gctcgtcgtc ctcgactacc
     6841 tgcagaccga cgcctcgtgg gacctcgaac aagccatcga caccctctcg gacctggaca
     6901 tggaggacct tctcgacccc gacaaggtcg cacacgcgct gcacctgtcg ggccacggtc
     6961 aggaggacga catggacgcc cacctccagc cccgcggcta tcggatgctg gcgcgcatcc
     7021 cccgcctgcc cgatgagctg gccgatcggc tcgtcgccca ctacgggtcg ctcggcaagc
     7081 tgagccgcgc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctggccga
     7201 tcggctcgtc gcccactacg ggtcgctcgg caagctgagc cgcgcgtcgg tcgaggacct
     7261 gtgcacggtc gacggcgtga ccgagcactg ggcgttgacg atcaaggacg cgctcggacg
     7321 catcgccgag tcgtccatcc tcgaccgcta caactgagcg gatcgtcggt ccggtcgtct
     7381 ggtcgggacc ccgcgaggcg gagcaccggc actagcgtcg gcgcccgtgg agaccctgcc
     7441 gtccggaacc cccgcccacc tcgcccgctc cgaacgggcg tcgcgggggc tgatcgtcat
     7501 ccccgacatc tggggcctgc gcccgctgtt caccgagatg tgcgacgacc tcgccgcccg
     7561 cacgggttgg agcgtcggca ccttcgagcc gttccccggc cgcgacctgc cgaacgaggg
     7621 cgagcccgac gccggaccgg cacgcttcgc ggtcctgccc gagctgtccg acgaccggct
     7681 gctcggcgat gcggtcgacc ttgcggatct caccgggtgc gacgacgtcg ggctcatcgg
     7741 gttctgcatg ggtgggatgt acgccctcaa ggcagcggcg accgggcgct tcgaccgcat
     7801 cgccgccttc tacgggatga tccgggtgcc cgaggcgtgg cagggcccgg ggcagggcga
     7861 tccgctcgac gcgctcgacg cccgcgggtc gaccgaggtc atggcggtcg tcggctccgt
     7921 cgacccgtac acacccaccg accacgtcca ggagctccgc gaccgcgggg tcgacgtcgt
     7981 ccactacgag ggggccgacc acggcttcgt gcacgacccg acccggccca gtcaccgggc
     8041 cgacgacgcg gccgacgcgt gggaccgggt cctcaccttc ctggcgggct gaacccggcg
     8101 tcggtcgggt gggtctgctc aggtcgagcg gatctccagc tcgcggcgct tgaggatccg
     8161 gtactggcgg tcgttctgct cgatccagcc gagccgcagg aagctggcga tcgccttgtt
     8221 cacccgctca cgggacgcgc cgaccaggcc ggccagctcc tcctgggtga tcgggatctc
     8281 gaagtcgtcg cggtccccgg cgagctcgag caggtgcttg gcggttcggc cggtgacatc
     8341 gaggaagaag gcgtcggcca acgcctcgtc ggtggcgcgg atgcgctggc tcagcatgcg
     8401 caccaccgac cacaggagct cgggcttgtc ctcccacagg gcctgcagca cgtcgtaggg
     8461 gatgcggatc acgctggact gctccagcgc ccgggcttcg gcagagcggc ccatcccgtc
     8521 gaagaacccc atctcgccga acaggtcgcc gcgctccatg agcgccacca tcgactcgcg
     8581 gccgtcgatc gacttgttgg cgatggcgac ccggccgtcg atgaccacgt agcaggcgtc
     8641 ggcctcggcg ccctcggtga acacgacatc gctgcggatg agatccaccc gctccgcggc
     8701 gtcggccacc cgccggtggt cctcgtcgtc gaagcggtcg aacagctcga cgttgtggag
     8761 caggtccatg tcgttcacga ccggcatcgt agtgacagag atcttcgtca cacctagtcc
     8821 gcgggcgggc cgttcccggc ccgttccgat gggttgtcga tcccgggatc ggtcgtggcg
     8881 gccggtacgg tgcgggcgtg agcgaggtgg cgacggggcc ggtgtggtgc gtggtggtcg
     8941 cggcgggtcg cggatcgcgc ttcggcggtc ccaagcagca ggcgatccgg gcgggccgga
     9001 ccgtcctgga gcgctcggtc gcaaccgccg gtgcggcctg cgacggtgtc gtggtggtcg
     9061 tgcccgacga ctggaccgac ccggtcgacg gtgccgatcg ggtggtggtc ggaggtgcga
     9121 cgcggtccgc gtcggtggcg tccgggctgg acgccgtgcc cgccgacgcc gcggtcgtcc
     9181 tggtgcacga cgcggcccga ccgctggcct cgcccgagct ctaccggagg gtgatcgagg
     9241 cggtccgatc gggcgccgac gcggtcgtcc cggtcgtggc ggtcatcgac accatccgca
     9301 gcctcgacgg gggcacggtg gaccgcgacc tgctgcgggc ggtgcagacc ccccaggggt
     9361 tccgggtcga agccctccgc gctgctcacc gctccggcgg cgacgccacc gacgacgcca
     9421 ccctggtcga ggcggtgggg ggatcggtgg agctggtcga cggcgagcgg acgaacctca
     9481 agatcaccga gccgtccgac ctggacgtgg cggaggcact gctccggcgg gccgctgcct
     9541 cgaccggagg cgacaccgac ggtgcccccg ggggagactc cctcctgtga gcatccgggt
     9601 cggcaacggc ttcgacatcc accgattcag cgacgccccc gaccgcgtcc tggtgctcgg
     9661 tggcgtgagc ttcccgggcg agcccggcct gcacggtcat tccgacgccg acgtcgtcgc
     9721 ccacgcggtg gccgaggcgc tgctgggcgc ggccgggctc ggcgacctcg gatcccactt
     9781 ccccgacacc gacgaacggt ggcgcggcgc cgacagcctg gagctgttgg ccgaagtcgt
     9841 gcggctgctg cacgaggatg gttggtcggc ggtcaacgtc gactgctcgg tgatcgccga
     9901 gcggccgaag ctcgcccccc accgtgatcg catgcaggtc gtgctcagcg acctcgtcgg
     9961 agcgccggtc acggtcaagg gccgtcgggc cgagggcatc gggggcctcg gccgcgccga
    10021 gggcatcgcg tgtctggcca ccgcactgat cgagacgcgg gtccacttcg tcgaccagag
    10081 gagcagctga tggccggtag gtcccccgga ggatcacgtc gaggagggtc ggaccggtcg
    10141 cgctcgtccg ggtcggggtc cgcgggacgt ggcgggaagg gctccggggc atcgggccgg
    10201 gcctccggcg gatcgcgggg gggtggtggt cccaagggtg ccggaccccg ccgcagcggt
    10261 ggcgcccggg gcggggcacc gtccgggcgc gccaaccctc cgggcagcga acgggcccga
    10321 cagggcgccg cggcgcgccg ggcgccgcgc aaggacccgg gtcgcacgac cgcacccaag
    10381 gagcgcgggc tcggcggcac ccaggtcgaa ggacggcagg ccgtgcggga gctgctgctg
    10441 gccgggcgtc gcaaggtcta cgaggtgttc ctcaacgccg agatggaccg ggccgacatc
    10501 gtcggcgaca tcgtggagtt ggccgacgag ctgagggtcc cgatcaccga ggtgagccgg
    10561 ggtcggctcg actcgatcag ccacaccgag gcaccccagg gcgtggtggc ccgcgccgcg
    10621 gagctgcccc acaccgcgct gcacgacctg tgcgagccgg tcgacggccg tccgccgttc
    10681 ctgctcgcgg tcgacggcgt gaccgacccc gggaacctgg gcgcgctgtt gcgcatcgcc
    10741 gagtgcgccg gggtgaccgg ggtggtgctg ccgcgtcacc gggcggtgca catcaccccg
    10801 acggtgacca aggcggcggc cggcgcggtc gagtacctgc gcatggcgct ggtcggcggt
    10861 ctgccgaccg ccatctccga tctgcgcgac gccggggtgt gggtcgtggg cctcgacgcg
    10921 gggggcgagg tgtcggtgca cgacctgccc gtcgctaccg acgcggtctg cctcgtcctc
    10981 ggcgccgagg gcaagggcct gtcgcggctg gccgcccagc gctgcgacct ggtggcgtcg
    11041 atcccgttgc acggccacct gtcgtcgttg aacgtcgcca gcgccggagc ggtcgcgtgc
    11101 tacgagatcg ctcgtcgtcg ccctcccgcc acggtgtgac ccggtcccgg tagcgtccgg
    11161 gaccatgagc gactcaacca ccaccgaccc gcgtcccgcc gccctcgacg ccgcccggca
    11221 caccgcccag gcggtccagg acgtcggtgc caagttcatg ttggacatgg acatgtacgg
    11281 cgaggtcgcc gggctcggct accagggcct cggcttctac atcgccggtc gcggcggcgt
    11341 gctgggcgac gtcgagcacg tcgaggtctt cgaggccatg accttcttcc cggccgagac
    11401 cgtgcaggcg ggctgggagt cgtccgcctc ggtcgagagc cgggccgact ccgctggtcg
    11461 cttcgccggc tacgcggcgc gctggggcgc gaacaacgtc gccgacggaa ccgagggcct
    11521 ggaccgactg gccgagctgt gcgagaaggt gatccaggcc gcggacggct ccgatgcccc
    11581 gctgttcgcc ggctggcgcg acctgcccga acccgacggc gaacccgagc tggtcgtgca
    11641 ccggatgaac gcgctgcgcg agctgcgcgc cgcgcgccac atcgctgcca tccgtcaggt
    11701 cgggctggat ccggcaacgg cgttcatgat ccgcacgccg tacatggcgg ggatcttcgg
    11761 ctggccggag ccgtccgagc ctcctgcgga gatcgacaag gacttgtggc agagcgccga
    11821 ggacctcacc gagcgggcct tcgcggcgga cctggccgtg ctcgacgacg acgagctggc
    11881 cgagttctgc cggctgaccg acgccctgct cgccgccatc ccgtagggcg gccaaggtcc
    11941 tggaacccgc cacccgcagc atcggtcggg ccggctattc ggcgggtccg tggacgcgca
    12001 ccgcgtcgcg gtgctgggtg tcgaggtccg tcagccgttc ggcgatgacg ttgcaggcgt
    12061 tggctaccgc cagcgcctgc aacgtccggg gataggggtc ggcccggtct ccctgctcga
    12121 cccgccggac ccggttcagc gcggccagcc gcaccggctc caggttggtc agctcgatcg
    12181 gactgtcggg atgggcgtgg cggaaccagc cgctgatgtc gtgcatggcc tgttggagcg
    12241 cgagcgtgag cgtggtcagc tcatcccggc aggctcgcag cagggcggag tcacccgcgt
    12301 cggccacctc ggtgctcagc accgcgcact gctcccagat ccggcgggtg gtcaccctcc
    12361 agtgcgaacc gagcaggccg tgcgacgggt gctcggccat ggcgtcgtcc cacagatcgt
    12421 cgaccttggc cagccgggcc cgcccgtcga tgcgcgcctg gcgcaccgcg tcggcccggc
    12481 ggggttggcc gtcgagcacc tcggtggccg acccgagcag gtgagccatg tcccggtaca
    12541 cgccgcccag ctcgcggcgc gcggtccgat gggcccgcgg cgggaacagc acggcctgca
    12601 ccgccatggc gacgacgatg ccgatcaccg cctggctgag tcggatcagc gcgaagtcga
    12661 aggtggagaa gtcggtgctc tcggggacca gcaccaccac cgacgccacc agcgccgcct
    12721 gctgaccgat cgccgggagc cggaaccacc cgaacagcac cagcggcgcg gcgaccacca
    12781 gggcgaccgc ccacgcctgg tccttggcga cgtaggccac gcccagaccg agcgcggccc
    12841 cgatgaacgc ccccacgagc gagttgcgcc cggagcggaa cgtcgcctgc agcgtgccct
    12901 ggatggcgac gatcgcggcg acggtcgcga accacgggtt gggcaggtgc agcagctgag
    12961 agcacagcgt ggccagcccg gcggcgaccc cgatgcggat cccgcgcttg atcacgcgcc
    13021 ggtgggcggg ggagatctcc ggagatcgga cggtcatcga ccctggatca tcccagccca
    13081 ccggccgcga cgggtcgatc agggggtgcg gccgttccag gcgaccaggc ggtcgatcag
    13141 cggggcgtcc tcggcgacgt cgaccgcgtc ggcgaacggg atgtcgcgcg ggacctccgc
    13201 gggcatgccc gcgtagatcg cctcccagat cggggcgcgg accgggatgg gcagttgggc
    13261 gtgcatgacc gcgagcgacg cctcgagcac cgccgggtcc cactcggggt gctggtcggt
    13321 cgcggtcgcc aggtcccagg tgtggatgac catctcgttg gtgtagacgt cgagcacctc
    13381 gcggccgggg aacgtccccc acggaagcgt cgtcgggcgt tcgagcagcg ccgggtcggc
    13441 ccacgcctgc tcgacgtcgc tcccggcgac gcgcagggca tcaccccagg tgccggcggc
    13501 gacgtccgcg gcgtcgatcg gccactcggc gagcgggacg tcccgaccgg cgagcgccac
    13561 gcgccgcagg gccatgacca ggtgctcggc gagtgcgccg accgacatgg gcgggcacgg
    13621 ggtgggacgg tcgtactggg cggggtcgag ggcgtcgatc gtcgtggcgg cgacgccgac
    13681 cgcggcgctc aggcgggcga gcggctccat cgaggtgctg ccgggggcgg tggtcgtgtc
    13741 gggagtcgtg gtcgtgtcgg gagtcttggt cgtgtcggga gtcgtggtca tggcggggtc
    13801 tcctggtggt cgggatgatc cggtcgtccg gttcaggagc agcatccacg agtgaagtgg
    13861 ccaggatatg gccacttcag atgagaagat cgggacgtgc gtgccgaccg actcgtggcg
    13921 atcctgctga tgctgcagcg tcgtgacgtg gtcaccgcgg ccgaggtggc cgaggagctc
    13981 gagatctcgg tgcgtacggc ccgtcgcgac ctcgaggcgc tggccatggc cggggtgccg
    14041 gtctacccgc agcggggccg cggcggcggg tggcgtctgc tcggtggcgg gcgactcgac
    14101 ctcagcggtc tgaccgcggg tgaggcccgg tcgctgttcg tcctcgtcgg cggccgaccg
    14161 gaggtcggcc ccgaggtgcg ctctgcgctg cgcaagctgg tgagggcgct gccggaaccg
    14221 ttgcgcgagg gagccgaggc ggccgccggg tcggtcgtgg tcgatcccgt cggttgggac
    14281 cgccatcgcg gcgagccacg gcggcccgat cggctcgacg acctggaggc cgcggtgatc
    14341 gacggcgagt gcctgcacct gggctaccgg gcgcgctccg gcgaggtgac cgagcgggtg
    14401 gtccaccccc tggggctcgc agccaagggc cgcaactggt acctggtcgc cgggaccgcg
    14461 acggggctgc gcaccttccg cgccgaccgg gtcgaacggg cagagcgcac cggggagccg
    14521 gtggtgcggc ccgatgggtt cgacctgagc gaggcgtggg cgcggatcgt gtccgacgtc
    14581 gatcagctcc gcaccccgct cgaggcgtta gggaccgcgg ttcccgaggc cttgccctac
    14641 ctgcgcatgg tcctcggacg ccgggtcgag gtcatcggcg acgaggccgc cgggtccgac
    14701 cccgccgggt ccgacctcgc cggttccgga cccgagtcgg caaccccgtc ggacggctcg
    14761 gtccgcatcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggatgc
    14881 ccgcggcgct ctcgtgtcgc tcagcgcggt ccgccacctc gacggcggcg agcagcggtt
    14941 ccgctggtcc gcccacctcg acgacgtcga ccccgactcg gtgacggcgt cgctcgccga
    15001 cggcgtcctg accgtgtccg ccgccacccg gccgggcccg gtcgcccgca ccgtcgaggt
    15061 gaccgtcgcc gatccgtcgg agcctgcggc gctgcccgcc gacaccccgt cggacgaggt
    15121 cgggtccgag agcgccgagc cgacagtcac cgcagaggac acatccgaag aggtctgacg
    15181 gttcccgtca cggcgatggg cggacccgac cgggtccgcc cgccgccgcg tccgggcatc
    15241 gacgcagggt gggggacgac cggccgctgc gccggacgct ccctattcgt ccggggcgaa
    15301 cgagtcgagg atcaactcga gtccgaccgc gaactcgtcg ccgaagtcgt agccgggctg
    15361 catcacgtgc tcggtggtca tctcgacgag ccacgggtac tcgtcgacgg gcatgtggtc
    15421 gagcacgccc tcggcgaacc cggcgatgtc gtcggggccg tcgaacggga gccccgcctc
    15481 ctggagggcg aacccgtaga cgaacgagtc gatcacggca tacacgtggg cggtgtcggc
    15541 gatgctgaac ccggcgaccc gcagggtccc gatcacggcg tcgtggtgac gcaggatcgc
    15601 gggcccgggc gccgcgcgtg actcgagcag gccgatgctc cacggatggc ggtgcagcac
    15661 ggcgcgggca gagtcggccc ggcgacgcat ctcggcccgc cagtcgccgc cgatgcgggg
    15721 aaggtcgatc tcggagaaca cgacgtcgac gatcccgtcg aggatctcct ccttgttccg
    15781 gacgtggtgg tagagggcca tgggcctgga ccccaggcga tccgcgaggg accggatcgt
    15841 cagggcggcc aagccgcgct cgtccgcgat ggcgaccgct gcgtccagca cccgttcccg
    15901 gctgaggcgg ggccgtcgcg gtgggtcggt cgtcacgtgc ttgaccgtag ccgcaccctc
    15961 gtacatagta cggtacggat ccgtacaaag tacggtttca gaccacccgc cagatctcca
    16021 cccgaccgac cgccggagcc gaccccatgc gcagcgaccg cgaactgcac accccgaccc
    16081 gtcccgaccc cggctccacc atgcgggcgg tggtccagtc cagctacggg ccacccgatc
    16141 agctccggat cgagaccctc ggcgtgccgc aggtcggcga caccgacgtg ctggtcgagg
    16201 tccacgccgc cggcgccggt cggggagcgt ggcacctgct gaccgggacg cccctgatgg
    16261 cgcgattggg catcgggctg cgccgacccc ggcgaccggt ggcggggacc gatgtcgccg
    16321 gggtggtgct ggcgacagga gcgaaggtcg accgcttcga accgggtgac cgggtgttcg
    16381 ggatcgggcg cggcaccatc gccgaaatgg ccgtagtccc cgcctcgaag ctggcgtcga
    16441 tccccccgaa ctgctccttc gcggaggcgt cgatcctggc ggagtcaggc ctcaccgcgc
    16501 tgcaggccgt ccacgacagg gcggaggtcc gacctgggga tcgggtcctg gtcctcggag
    16561 cctcgggcgg tgtcggatcg ttcgcggtgc agatgtccgc ggggctcggc gcccgggtga
    16621 ccggagtgtg ccggagcgac aagacctcag cggtccgggc gttcggcgcg gacgaggtcg
    16681 tcgcctacga ccgcgccgac atcaccgagc gcggtgaccg ggagctgttc gacgtgatcg
    16741 tcgacgccgg cggccgtcac ccgctccggc gcctccgacg gatcctgacc cctcggggcc
    16801 ggctcgtcat cgtcggtggc gaaggcgggg gtcgcatcac cgggggcttc ggccgcggct
    16861 tccgcgccgc cgcgctgtca ccgttcgtcg ggcagcgcct gaccatggtc gtgtcgaagg
    16921 agacgtccgc ggacctggag cgtctcggtg aactggtcgc cgacgggacc gtgcgtcctc
    16981 cgctggagcg aaccctcccg ctcggccggg ccgccgacgc gttcgctctg ctggaggccg
    17041 gcgaggtgac cggcaaggtg gcggtcacgg tttgcgcgcc gtccggcgac gacgggtgag
    17101 cgttcatctg agctcgggat ccacgtcgtg cgctcggtga aggtgtgcag aagagccggg
    17161 aggggcagcc gcgttcatgt cggctacgac gtcggcatcg ccgaactggg acgtggctgg
    17221 cctgggaaca gcggtaatca gcgctcccgt ccccagcccc tacccgaagt tgtaagtcat
    17281 cctgccgatc tcatggctcc cgagaccctc agacgcgacc aactccacgg gcgtggtctg
    17341 gtcgtcgagt tcgtaggcaa ccgcactagc gaccgtgccg cccttcttga ttttcaccat
    17401 ctggttctcc aggaactgcg gatccgggag cgaagccagg tcaagttcat tcacagcgtt
    17461 gggatcgttg tcctgatagg cggagaacac gaagacccag ttcatggggc tcacgtcgcc
    17521 atccgtcagg ttggttgttt cgtaccagaa ggcaatgacc ggtttgtcgc catactcatt
    17581 tccaggttct ccgacgggaa tcaccttctt gtcagtgatc ttgatgatca tcttcggggt
    17641 cgtaagtact ccgtccacga aggaggattc gctcccagag gcagtgcttg gctcggctgg
    17701 ggcgaccgac tctgcagttg ttgacgtgct ggaagcgatc tccttgtcga tggattcagc
    17761 ggccttgtct accgccgcac cacccgcgac caagagaaca ccacaaccgc caacaaggag
    17821 tacgccaaat gcccccaagg cgatcagcac gttgcgagcc atgtgggact tcttcggcgg
    17881 ctgcggactc aactgagcct ggcctggctg agcagacggt gatggccccg ttgttgaatg
    17941 ctgctcggtt ggtgctccgg gagcctgctc gggcggatac cacttgccgt ccgatgcctg
    18001 ccaccaaccc tgtccttggc tcgtgtcgct catcttggag tccccgcaga tcggctgtga
    18061 tcgggacgat cccgaccgtc ctctcagcgt aaaggcgacc agacgagatt ccacgcgctg
    18121 tcgaaagaca atcgcgatgt caccccagac cagtccgtcg agaaccaact cgtcgctctc
    18181 ggaagtcgag cattccggcc cgcctgcaac ggctggctcg aacttggcgc aacgcctgac
    18241 gcgattccac gaagggcgcc ccgacgcgat cgatgccgtg gttggctgtg ctcaccaata
    18301 gtgcatcccg acttggtgac cttgcaggcg tggcaaaccg cagcattctg gcgcttcgga
    18361 gttgcggggg gtgtgggcgg gtagcggggg tccgcggttg agg
//
LOCUS       DAOQOD010000049        17829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1110_length_17829_cov_10.930516, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000049 DAOQOD010000000
VERSION     DAOQOD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_17829_cov_10.930516"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="groEL"
                     /locus_tag="PLZ72_06720"
     CDS             <1..1088
                     /gene="groEL"
                     /locus_tag="PLZ72_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNA36026.1"
                     /translation="DFTEGMQFDKGYLSPYFVTDAERQEAVLENPYVLFVNGKIGSVQ
                     DLVPVLEKVMQAGKPLLIIAEDVEGEALATLVVNKIRGTFNSVAVKAPGFGERRKAML
                     QDMAILTGGQVIAEEVGLKLDNTTLDLLGQARKVVITKDDTTIIEGAGSGEDVAGRVA
                     QIKAEIDNTDSDWDREKLQERLAKLSGGVAVVKVGAATEVELKEKKHRIEDALSATRA
                     AIEEGVVAGGGTALVRAKAAVASAVEALDGDERTGAGIVLRSLDAPARLIADNAGLEG
                     AVVVQQLERETGSVGLNAATGEFEDLLKAGVIDPAKVTRAALQNAASIASLLLTTECL
                     VADKPEEAAAGGGMPDMGGMGGMGGMM"
     gene            1258..1419
                     /locus_tag="PLZ72_06725"
     CDS             1258..1419
                     /locus_tag="PLZ72_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36027.1"
                     /translation="MENRPAPDPAKLLQYWMEWERGETPPGRTMSNLKTHGLRDLLEG
                     LVEASSGAA"
     gene            1422..2171
                     /locus_tag="PLZ72_06730"
     CDS             1422..2171
                     /locus_tag="PLZ72_06730"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNA36028.1"
                     /translation="MSRGGPQQIPRPEIWTPGRPAPWSQLDPQSRLILPDRVRSVYPR
                     DRLGLAAPVAADGAEPSAVLVPIYDLDGELHVVLTRRSWNLRTHRGEVSFPGGRADPG
                     EDHVTTALREANEEIALDPALVEPLGELDHLTTISRRAYIVPVVAMVSRVPELRPNDA
                     EVDRVLHVPLSELTADGVFREERWGEGPTSRPIYFFDLDGDTVWGATAAMLRQLLARL
                     TGTDPGTPIDMDPARDIPPGFLLPGETDRVV"
     gene            complement(2195..2602)
                     /locus_tag="PLZ72_06735"
     CDS             complement(2195..2602)
                     /locus_tag="PLZ72_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA36029.1"
                     /translation="MTGPVFVDTSAIYAVLDPHDRQNFTAGEILVDLITSGSTMVTHS
                     AVIHECSALVQRRLGMAERRDLHGRLLPLFDIEWVDAAISDRAVSALMAADRRDASLV
                     DWTSFEVMRRRAIRIAFAFHDDFVAQGIDVLAR"
     gene            complement(2599..2808)
                     /locus_tag="PLZ72_06740"
     CDS             complement(2599..2808)
                     /locus_tag="PLZ72_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36030.1"
                     /translation="MRRLSTLRQKSQAALLRDALDQLLAHDERERLTEMALEVISRYR
                     GSTPEDGQTSIAEDHNAALEDAYLS"
     assembly_gap    2916..3015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3025..4326
                     /locus_tag="PLZ72_06745"
     CDS             3025..4326
                     /locus_tag="PLZ72_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNA36031.1"
                     /translation="MVASQDHMGTVDFMGPYQRRIIDDELDELFTQLPAILLDGPKGV
                     GKTSTAEQRCVTVRRLDVEAERQVVAADPSVIAGDARPLLLDEWQRVHPTWDAVRRLV
                     DADSTGGQFILTGSAPTKESHSGAGRITSMRMRALTLPERGVAETSVSLAALLGGNGD
                     VGGRTEIALADYVAEMVAGGFPGLRHLEGRALTRQLDSYIERIASHDLPEAGFAVQRP
                     ATVMAWLRAYAAATGTTASWEKIRAHATVSADARPAKTTTQPYIELLTALRILDPVTA
                     WIPSNNHLGALTLAPCHHLADPALAARLVKRSATRLLRGDAPDPLQPRDGGFLGGLFE
                     SLATLSVRVFAQASDARVHHLRTEKGRREVDLIVETDEGVLGVEVKLGAAVGDNDVRH
                     LVWLRDKLGDECIDTLVLHTGPEAYRRTDGVAVVPLALLGP"
     gene            complement(4544..5428)
                     /locus_tag="PLZ72_06750"
     CDS             complement(4544..5428)
                     /locus_tag="PLZ72_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA36032.1"
                     /translation="MLIAQISDPHVTTEGTVVRSLVDTPQRFADALATCAALPKVPDV
                     ILLTGDLVNDGTAEEYALLAEALAEAPCPVLPIPGNHDDPLLLHATFAALDVCGAALP
                     DPRSGPFHYVVDDHPVRLVALDSTVAGHHHGQVTEQSAAWLDATLTAAPERPTMLFMH
                     HVPYATGAWWFDYSGIDGRDLLREVLVRHPQVLRVVSGHVHRATSTQWGHLTLSSAPS
                     TAFLSGTGVGDGPPLIVDQRAAPQLFAVDGDSLVASEADLPGLRLSIDMRDLVQDWST
                     YESVTRSRGPVSKDMFGG"
     gene            complement(5547..11645)
                     /locus_tag="PLZ72_06755"
     CDS             complement(5547..11645)
                     /locus_tag="PLZ72_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA36033.1"
                     /translation="MLPFLIILVGCAALVPLQWQRLRPPGPRTRQRPALGATAVVLAG
                     AVTVGSLSATFRPADAEDAPGDADAATLAGDRMVTAPMAGYQLADAFGEVPGVTQVDP
                     VGIADDGTIVGNYVHEGRPKAFRARPGQPFEELYAAGAERITADAVAPDGTIAGHIAT
                     LSAPDADGWVGVWDPDDEGNLGDFTRRLAQPKGAPTVETVRAVNSRGDVLLGTSVLHP
                     DGNRTLLAHPQPGGSNPQLSALDMNETGQVVGFATWTEFNQPVTRAVQWNAGVPVLQL
                     QFGQVSAIADVIADDGTIGMRAATGTPEQGYRTWVVAPGVGPATPVAPLGQEVLLRDI
                     NERRTAVGMVMADGALRPVAFDGTLKRLDRLVDEPTVHLQSAIGINTDGVIAGDVDWQ
                     GAASVFLARPVNPVVFVHGAGASRISQTAPRTADRGDEGDQEWIRCGADRMHLSLWPE
                     DVQAGRALTNLAAFAPLRDETCYGIGSWADDALGVYDTLLSHIEGTGFRPYRVDARLD
                     RYTTAGCDTSQNSPNLFTFAYDWRLDNGDSGRRLADFMGCVRRFWPDRDVNIVTHSMG
                     SLVAQRYLLDAGDDAPVDRLITFGAPWLGSPKLVNVLYTGDFAAGPVNGPAGIIRHIV
                     GSFPAAHQLMAGPYYHQLATRPVFRELGADRDLDGVDRDDFTFAHLKQMLDAENPTFT
                     PGTVAESFQTQAGQADWSSLDTDVDITHFVGLQAGRNTIGTVVYRTRPVCDLSSFSCE
                     PRTVVEQSLVCGDGTVPLVSAARVGRGRDLNAPDAEIQVLTSPSADENGRAEHTGMTT
                     NPAALGRLDQLLATPSAKDGPGTYSAPLGAEDQDTASCGGSANGAGLAAAGQGAPDRA
                     HSLRYLELLGGSDVTVTDDLRHVTPMADGSTGTVPGVTQFRSNAEDAGLTTLPLSSTR
                     TYRVAFHATGDPLQLELLDGTQDNPTRAMRWTDVQVPEGAAELEMAPERTPVLRIDSD
                     GDGEVDSPVAPTVDLSGPAAADVTAPELTVTAVGTGEDRRYAVSATDDGPGAIDVLVS
                     TGGRFAPYDGPFSAPAGTTTIRAFATDAAGNRSPMTDVAIGSVPTTPLTTGTRTREAN
                     PRGWNTGPVDVALRAEAAGGVASVTWWAEGATATAPSTVAGDRADVAVTATGVTHLRF
                     RATGADGTVEPVRTLTVRIDDDDPSVQLQTPYADSTVADLDRIAGTAADATSAPAEVD
                     VEILDPDDRVWDGDSWEDVGDGEHRWLNATVTTGPAGTATFERRSANPSGADLPPGGY
                     RIGLRVTDGAGHRSVTEPARVTVAADAAWQAVELAPLGPSQTSSARAVDDRGAVLGTT
                     TDGGTTDVVWAGGTVTALPVAEDAEVTARSIDGSMAGSVSSGGGGSRAFEWSPTGTPT
                     ELALTPGTTSSWAVDRNDAGTVIGMTGNAGVFVDAVRWDDDGAHRLALPFTGVPATTD
                     INSGGAIVGWIDPPDIGDVTPRRAVRWDPDGTAHPLGTLGSPSRAPSTATAVNDLGVV
                     VGSAWTDVEIAGDQHAVVFRDGRAVPVDAGLFADGATATDVNDRGWIVGDYTTRTGSL
                     PRAFLSTDAESAVDLNTLLPDGSGWVLERATGINDLGQIVGRGRLDGVPRGFVLSTLH
                     APVAEDVSVTAAGETRITLDGWDPDPTDTLTHHLVGTASHGSVELLDGGVVRYRPQRG
                     FTGTDRFDYRVEDGRFRSDSATVTVTVTEDPTGPDNREPLATITLPATGIEGTSVTAD
                     GSASSDPDGDELTFAWDLDGDGAFDDGTAPTATTTLLDDGPHSVALRVTDPDGATASA
                     TATLTVLNSAPTIGGLRGTASVTAGTPLELSGTVDDVPADTHTAVLDPGDGSAPAPVA
                     VTDGRFTLRHTYTTAGVHDAVLSVVDDDGAHAEARITVTVEAPERVLAPATISVAGSV
                     AVPGSGTRPDRIGLVGAVIAPKGSTGSTWGAIGIARTGSTPMALGSVRVTRAGTYTEG
                     GVRYGVVIGTGVLGSGRTARSVTYELTVADGRPDRVWLKVTDSSGAAVTGLTVGGTPP
                     GGGLTFLSGSASVTPR"
     gene            11821..12960
                     /locus_tag="PLZ72_06760"
     CDS             11821..12960
                     /locus_tag="PLZ72_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNA36034.1"
                     /translation="MMSQLTAERVRRDIEVVSRAGLPLPEFLTEVDGSLRRAVPYRAM
                     CSALFDPATHLCTATVKLGELAADEEADARWGTIEYGYDNPTNFAALSAAARPATSAR
                     LEALNEPDVLFRAREFLHPQYGFSDELRSIARADGHTWGGFALHRRDGDPDYTEEDIA
                     FVGALSSDLAVGFRSALLVGRVGELDAPRDLPAAPAVLIVHGDDVLGQMNDGASAWLD
                     DLGVTPEDTGTSSVISGLVAAARRLAAGTGTMTPRLRVRGRSGRWLVLHSSPLSGPTS
                     SGTDVVVTIEEARPPEIVAIVVAAFDLSPRERDVTQLVLQGVDTRGIATALSMSRYTV
                     QDHLKSVFEKADVRSRRELVSRVYFDQYVPRMGSDIGADGWFVGT"
     gene            complement(12984..14534)
                     /gene="guaB"
                     /locus_tag="PLZ72_06765"
     CDS             complement(12984..14534)
                     /gene="guaB"
                     /locus_tag="PLZ72_06765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNA36035.1"
                     /translation="MAPLSDDSPAPSASPSTAPAGLSAAIGDPSDRFGSMGLTFDDVC
                     LVPGPSDVIPAEVDTSTRLTPNIVLAIPLMSAAMDTVTEARLAIAIAREGGLGVIHRN
                     LSIDDQVTEVDKVKRSESGMIVDPVCLGRDHLVADALDLMAKYRISGVPIVDDDHHLV
                     GILTNRDLRFEDDPSKRVDQVMTAEGLVTAPQGTTLEEAQAILGRHRIEKLPVVDDDG
                     RLTGLITVKDINKKLKYPNATKDPQGRLRCAAAVGVGDDALERAQALVDAGVDLLVVD
                     TAHGHSAGVLEVVRKIKANLNVDVVAGNIATGAATAALIDAGADAVKVGVGPGSICTT
                     RIVAGVGVPQLTAVQECARVAAAHGVGIIADGGVRYSGDIAKAIAAGADVVMVGSLLA
                     GVDETPGEIVLSQGERYKAYRGMGSLGAMNARSFSKDRYFQGEVETTEKVVPEGVEGR
                     VPYKGPAQKILYQLIGGLRSAMGYCGSHTIGELQTGAQFVRMSPAGLREAHPHDVTIT
                     AEAPNYWM"
     gene            14597..15913
                     /locus_tag="PLZ72_06770"
     CDS             14597..15913
                     /locus_tag="PLZ72_06770"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA36036.1"
                     /translation="MERRRRVVVVGAGFAGLELVKGLAAAPVDVVLVDANNFHTFQPL
                     LYQVATAGLDGDDIAAPVRAVVRSQANVDVRLGQVVDIDVDDRRVVFRDGPALSYDRL
                     VLAAGAVTNTYGVPGVDEHGFGLKSLEDALQLRQHLLRQFERASVDPSLVEQGVLDVV
                     IAGGGPTGVELAGGIAELYRNVLSHDFPTLDVRKARVVIVEPGDRVLAPFHPKLSARA
                     ARTLARLGVELVFGQGVTGTDGKVVQLDDGSSVPCSTLVWTAGVKASPLADVLARHLG
                     DGTLTRGGRVVVTPELTVPGHPEIAVVGDLAASPDGSGGVLPQLAPVAIQGGRLVAAN
                     LVAELAGHPAEPFHYVDKGTMATVGRRNAVAQLPGRLRFSGTPGWLAWLGLHLIMLIG
                     FRNRANVMVNWAWNYVTYDRASRLIVGDDDHPARPSGRPMPTDPDR"
     gene            15964..16353
                     /locus_tag="PLZ72_06775"
     CDS             15964..16353
                     /locus_tag="PLZ72_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36037.1"
                     /translation="MPSVTFEGETHGEIVVKVRRWLASVDGQEEGGLTAAEAVTQGAE
                     LTKDALRIIASAAPEPVAQSDLVKGLTNMGYKATDATRSAVIAGLDSIEEMTGGGVVH
                     QASDVGRRVVWQMNTSMAKQLLRSLRS"
     gene            16468..17628
                     /locus_tag="PLZ72_06780"
     CDS             16468..17628
                     /locus_tag="PLZ72_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HNA36038.1"
                     /translation="MAEIEIGLGKDGRRGYSLDEVAIVPSRRTRDADEVETSWQIDAY
                     QFALPVIAAPLDGVTSPATAVEMDRLGGVGALHAEGLWCRHDDPTALLERIAAHDPRD
                     GEGDDRDGVALLREAYDAPVRPELVEQRIAEIRDAGATVCVAVSPAHTEALLPTIKKA
                     EPDLLMIQGTVTSAEHVSGGAHDPLDLKHLVRRLEVPVVVGGCASYQAALHLMRTGAA
                     AVLVGIGTGATSTTGRVLGVGNPQATAVADARAARMRHLDETGVYVHLIADGGMATGG
                     DIAKAVVCGADAVMLGAPLAAADEAPAGGAVWSMTSFHSRLPRGRLRRVPRRGSLEQI
                     LLGPSDRADGRTNLMGGLRKAMAVTGHGTLKDLQKAELVVTAPPAVRPGAQR"
BASE COUNT         2380 a         6121 c         6689 g         2539 t
ORIGIN      
        1 tcgacttcac cgagggcatg cagttcgaca agggctacct gtcgccgtac ttcgtgaccg
       61 acgccgagcg ccaggaagcg gtgctcgaga acccgtacgt gctgttcgtc aacggcaaga
      121 tcggctcggt ccaggacctg gtccccgtgc tcgagaaggt catgcaggcc ggcaagccgc
      181 tgctgatcat cgccgaggac gtcgagggcg aggcgctggc caccctggtg gtcaacaaga
      241 tccgcggcac cttcaactcg gtcgcggtca aggccccggg cttcggcgag cgtcgcaagg
      301 cgatgctgca ggacatggcg atcctcaccg gcggtcaggt catcgccgag gaggtcggcc
      361 tcaagctcga caacaccacc ctcgacctgc tcggtcaggc ccgcaaggtc gtcatcacca
      421 aggacgacac caccatcatc gagggcgccg gctccggtga ggacgtggcc ggtcgggtcg
      481 cccagatcaa ggccgagatc gacaacaccg actccgactg ggaccgcgag aagctccagg
      541 agcgcctcgc caagctcagc ggtggcgtgg ccgttgtcaa ggtcggcgcc gccaccgagg
      601 tggagctcaa ggagaagaag caccgcatcg aggacgcgct gtcggccacc cgcgccgcca
      661 tcgaagaggg tgtggtcgcc ggcggcggca ccgccctggt ccgtgccaag gccgcggtcg
      721 cctcggccgt cgaggccctc gacggtgacg agcgcaccgg tgcgggcatc gtgctgcgct
      781 ccctcgacgc cccggcccgg ctcatcgccg acaacgccgg cctcgagggt gcggtcgtgg
      841 tccagcagct cgagcgtgag accggctcgg tcggcctcaa cgccgccacc ggcgagttcg
      901 aggacctgct caaggccggc gtcatcgacc cggccaaggt gacccgtgcc gcgctgcaga
      961 acgcggcgtc gatcgccagc ctgctgttga ccaccgagtg cctcgtcgcc gacaagcccg
     1021 aagaggctgc cgccggcggc ggcatgcccg acatgggcgg catgggtggc atgggcggca
     1081 tgatgtgatc aggcccgcct gacgtcgacg gccccgccct ccgggcgggg ccgtcccgcg
     1141 tccggcgata taggggtccg cactcacaac ccttgtcaca atcccgacca gagaatccga
     1201 cgtcgtgtcc gggtcgcccg gcgcggtcct gacccgggcg ggcatatcgt gccggggatg
     1261 gagaatcgtc cggcccctga ccccgccaag ctgctgcagt actggatgga gtgggagcgt
     1321 ggcgagacgc cgccggggcg gacgatgagc aacctgaaga cccacggtct gcgcgacctg
     1381 ctcgaagggc tcgtcgaggc gtcgtcgggc gcggcctgaa cgtgtcccgc gggggcccgc
     1441 agcagatccc tcggccggag atctggaccc ccggccgtcc cgcgccgtgg tcgcagctcg
     1501 acccgcagtc gcggctgatc ctgcccgacc gggtgcgttc ggtgtacccc cgcgaccgcc
     1561 tcgggctggc cgctccggtc gccgcggacg gcgccgagcc ctcggcggtg ctggtgccga
     1621 tctacgacct cgacggcgag ctgcacgtgg tgctgacccg ccgcagctgg aacctgcgca
     1681 cccaccgcgg cgaggtcagc ttcccgggcg ggcgcgccga tcccggcgag gaccacgtga
     1741 ccaccgcgct gcgtgaggcg aacgaggaga tcgccctcga cccggccctg gtcgaaccgc
     1801 tcggcgagct cgaccacctg accaccatct cgcgccgcgc ctacatcgtg ccggtggtgg
     1861 cgatggtgtc gcgggtgccc gagctgcgtc ccaacgacgc cgaggtcgac cgggtcctgc
     1921 acgtcccgct gtcggagctc accgcggacg gcgtgttccg cgaggagcgc tggggtgagg
     1981 gcccgaccag ccggccgatc tacttcttcg acctcgacgg cgacaccgtg tggggcgcga
     2041 ccgccgccat gttgcgccag ctgctcgccc ggctgaccgg caccgacccg ggcaccccga
     2101 tcgacatgga cccggcccgc gacatcccgc ccgggttcct cctgccgggg gagaccgacc
     2161 gggtcgtctg agcgggtgct ccgaccgacg ccggtcagcg tgccaggacg tcgatgccct
     2221 gggcgacgaa gtcgtcgtgg aacgcgaacg cgatgcggat cgcccgccgg cgcatcacct
     2281 cgaacgacgt ccagtcgacc aggctggcgt ctcggcgatc ggcggccatc agcgcgctga
     2341 ccgcccggtc gctgatcgcg gcgtcgaccc actcgatgtc gaacagcggc aggagccggc
     2401 cgtgcagatc ccgtcgctcc gccatcccga ggcggcgctg caccagagca ctgcactcgt
     2461 ggatcaccgc gctgtgcgtg accatcgtcg atcccgacgt gatgaggtcc accaggatct
     2521 ctcccgcggt gaagttctgg cggtcgtggg ggtcgagtac ggcgtagatc gcggacgtgt
     2581 cgacgaagac gggtccggtc acgagaggta ggcgtcctcg agcgcagcgt tgtgatcttc
     2641 agcgatgctc gtctggccgt cctcgggcgt tgaccctcgg taccggctga tcacctcgag
     2701 cgccatctcg gtgagccgct cccgctcgtc atgggcgagc agctggtcga gggcatcacg
     2761 caggagggcg gcctgggact tctgtcgcaa cgtggaaagg cggcgcatga cgagcagttc
     2821 gcagggatga cataggaggc aggtggcgcg aacacccggg gttgcaagcg agtgccgaaa
     2881 cggtagcgtt ggaagtgccg aaacggtagc gtcagnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnagtgc cgaaatggta gcgtcgcagg atcatatggg gaccgtggac
     3061 ttcatgggcc cgtaccagcg aaggatcatc gacgacgagt tggacgagct gttcacgcag
     3121 ctgccggcca ttctgctcga cggcccgaag ggggtcggaa agacctccac ggcagagcag
     3181 cgctgcgtga cggtgcggcg gctcgacgtc gaggcggagc ggcaggtggt cgctgccgac
     3241 ccgtcggtga tcgccggcga cgcccgaccg ctgctgctcg acgagtggca gcgtgtccac
     3301 cccacgtggg acgccgtgcg ccgactggtc gacgcggatt ccaccggagg gcagttcatc
     3361 ctcaccgggt cggccccgac gaaggaatcg cactccgggg caggacgcat cacctccatg
     3421 cgcatgcggg cgctcaccct ccccgagcgt ggcgtcgcgg agacatcggt ctcccttgct
     3481 gcgctgctgg gtgggaacgg tgacgtcggt gggagaacgg agatcgcact ggcggactac
     3541 gtcgcagaga tggtggccgg cgggttccct ggcctgcgac acctggaggg ccgggccttg
     3601 acgcgccagc tcgacagcta catcgaacgg atcgcgagcc acgacctacc cgaggccggg
     3661 ttcgcggtcc agcggcccgc gactgtcatg gcctggcttc gcgcctacgc ggcggcgacg
     3721 ggaacgaccg cgtcctggga gaagatccgg gcccacgcga cggtctcggc cgacgcacgc
     3781 ccggcgaaga ccacgacgca gccgtacatc gaactcctca ccgcgctccg catcctcgat
     3841 cccgttactg cgtggatccc ttccaacaac cacctcggcg ccttgaccct ggcgccctgc
     3901 caccacctcg cggatccggc cctggctgcc cggctcgtga agcggagcgc tacgcgcctg
     3961 ttgcgtggag atgctccgga cccgttgcag cctcgcgacg gcggcttcct cggtgggctc
     4021 ttcgagagcc ttgccacgct ctcggtcagg gtcttcgcgc aagcatccga cgcgcgggtg
     4081 catcacctgc gcaccgagaa gggccgccgt gaggtcgatt tgatcgtcga gacggacgag
     4141 ggggtgctcg gggtcgaggt gaagctgggg gctgccgttg gcgacaacga cgtacgacat
     4201 ctcgtctggc tgcgcgacaa gctcggcgac gagtgcatcg acacgttggt gctccacacc
     4261 ggccccgagg cctaccggcg aaccgacggc gtcgcagtcg tgccgctggc actgctcgga
     4321 ccctgacagg tcaggggtac gttcctgcca gcaatgtcac ctgcaaactc gacgaatgtc
     4381 acccgcgaat cgccgacatt ggagacgcga gattcttcga atgccgagcg caactcaaca
     4441 cgtgagcctg ctgatcgaca cgctcgcctg cccgtagcgg cggcggtccg cggaacggat
     4501 cgcccggccg gtggctccgg acgtggtcct cccgccggtg gcctcagccg ccgaacatgt
     4561 ccttggagac cggtccccgg gaccgggtga ccgactcgta ggtcgaccag tcctgcacca
     4621 ggtcgcgcat gtcgatgctg agccgcagcc ccggcaggtc cgcctcggag gcgaccagag
     4681 agtcgccgtc gacggcgaac aactgcggag cggcgcgctg gtcgacgatc agcggcggac
     4741 cgtcgccgac gccggtcccg ctcaggaacg cggtcgacgg cgcgctgctc agggtgaggt
     4801 gaccccactg ggtcgacgtg gcgcggtgga cgtgtcccga caccacccgc agcacctggg
     4861 ggtgacggac cagcacctcg cgcagcaggt cccgtccgtc gatgcccgag tagtcgaacc
     4921 accacgcccc ggtcgcgtac ggcacgtggt gcatgaacag catcgtcgga cgttccggcg
     4981 cggcggtcag ggtggcgtcg agccacgccg cgctctgctc ggtgacctgc ccgtggtggt
     5041 ggccggcgac ggtggaatcg agggcgacca ggcggacggg gtggtcgtcg accacgtagt
     5101 ggaacggtcc ggaccgcggg tcgggcagcg ccgcaccgca cacgtcgagc gcggcgaagg
     5161 tggcgtgcag cagcaacggg tcgtcgtggt tgcccgggat cggcaggacc gggcacgggg
     5221 cctcggccag cgcctcggcg agcagcgcgt actcctcggc ggtcccgtcg ttgacgaggt
     5281 cgccggtcag caggatcacg tcggggacct ttggcaacgc cgcgcacgtg gccagcgcgt
     5341 cggcgaagcg ctgcggggtg tcgaccagcg accggaccac ggtcccctcg gtggtcacgt
     5401 ggggatcgga gatctgggcg atcagcatgg gccgagtctg ccccgccgca ctcgacggcg
     5461 cagacgccgg gcggcgaacc ggctgccgtc ctcgcggctc ggggccggga ggacgacagc
     5521 cggatccacc ggggcagaac tgaacctcag cgaggggtga ctgaggcgct gcccgacagg
     5581 aatgtgagcc ctccgcccgg cggtgtgcca cccacggtca gcccggtcac cgccgcgccc
     5641 gagctgtcgg tgaccttcag ccacacccgg tcggggcggc cgtcggcgac ggtgagctcg
     5701 taggtgaccg accgggcggt ccgcccggag cccaggacgc cggtgccgat cacgacgccg
     5761 tagcgcacgc cgccctcggt gtaggtgccg gccctggtca ccctgacgct gccgagcgcc
     5821 atcggcgtcg agcccgtccg tgcgattccg atcgcccccc acgtcgaacc cgtcgagccc
     5881 ttcggggcga tcaccgctcc gaccagaccg atgcggtccg gccgggtccc gctgccgggc
     5941 acggcgaccg aaccggcgac gctgatcgtg gccggggcga gaacccgctc cggggcctcg
     6001 acggtgaccg tgatgcgggc ctcggcgtgg gcgccgtcgt cgtcgacgac gctcaggacc
     6061 gcgtcgtgga ccccggcggt ggtgtaggtg tgccgcaggg tgaaccgacc gtcggtgacg
     6121 gcgaccgggg ccggcgccga gccgtcaccg gggtcgagca ccgcggtgtg ggtgtcggcg
     6181 gggacgtcgt cgacggtgcc cgacagctcg agcggagtcc cggcggtgac cgacgcggtg
     6241 ccccgcaggc cgccgatcgt cggcgccgag ttgagcaccg tcagggtcgc ggtggcgctc
     6301 gccgtggcgc cgtcgggatc ggtgacccgc agcgccacgc tgtggggtcc gtcgtcgagc
     6361 agcgtcgtcg tcgcggtcgg tgccgttccg tcgtcgaagg cgccgtcgcc gtcgaggtcc
     6421 caggcgaagg tcagctcgtc gccgtcggga tcgctcgacg ccgagccgtc ggcggtgacc
     6481 gaggtgccct cgatgccggt ggccggcagc gtgatcgtcg ccagcggttc ccggttgtcg
     6541 ggtccggtcg ggtcctcggt caccgtgacg gtcaccgtcg ccgagtcgga gcggaaccgt
     6601 ccgtcctcga cccggtagtc gaaccggtcg gtgccggtga agccccgttg cggtcggtag
     6661 cgcaccacgc cgccgtcgag gagctcgacc gagccgtgcg acgcggtgcc gaccaggtgg
     6721 tgggtcaacg tgtcggtggg atcagggtcc cacccgtcga gggtgatgcg ggtctcgccg
     6781 gcagcggtca ccgacacgtc ctcggcgacc ggggcgtgca gagtcgacag cacgaacccg
     6841 cggggcacgc cgtcgagccg gccgcgcccg acgatctgac cgaggtcgtt gatgccggtg
     6901 gcccgctcca gcacccagcc cgacccgtcg ggcagcagcg tgttgaggtc gacggccgac
     6961 tcggcgtcgg tcgacaggaa cgcccggggc agcgaaccgg tgcgggtggt gtagtcgccc
     7021 acgatccagc cccggtcgtt gacgtcggtg gcggtggcgc cgtcggcgaa gagcccggcg
     7081 tcgaccggaa cggcccggcc gtcgcggaac accacggcgt gctggtcgcc ggcgatctcg
     7141 acgtcggtcc acgccgagcc gaccacgacc ccgaggtcgt tgacggcggt cgccgtgctc
     7201 ggtgcccgcg acgggctgcc cagcgtgccg agcggatggg cggttccgtc ggggtcccag
     7261 cgcacggcgc ggcggggggt gacgtcgccg atgtcgggcg ggtcgatcca gccgacgatc
     7321 gctccgcccg agttgatgtc ggtggtggcc ggcaccccgg tgaagggcag cgccaggcgg
     7381 tgggcgccgt cgtcgtccca gcggacggca tcgacgaaga cgccggcgtt gccggtcatg
     7441 ccgatcacgg tgcccgcgtc gttgcggtcg accgcccagc tcgacgtcgt ccccggggtg
     7501 agcgccagct cggtcggggt tccggtcggg ctccactcga acgcccggct cccgccgccg
     7561 cccgaggaga ccgatccggc catcgacccg tcgatcgagc gggcggtcac ctcggcgtcc
     7621 tccgcgaccg gcagagcggt cacggtgccg ccggcccaca ccacgtcggt ggtgccgccg
     7681 tcggtcgtgg tgcccagcac ggcgccgcgg tcgtcgaccg ctcgggcgct cgaggtctgc
     7741 gacgggccga ggggcgccag ctcgaccgcc tgccacgcgg cgtcggcggc cacggtcacc
     7801 cgggccggct cggtgacgct ccggtgtccg gccccgtcgg tcacccgcag accgatgcgg
     7861 tagccgccgg gaggcaggtc cgctcccgac gggttggcgg agcgacgttc gaaggtcgcg
     7921 gtgcccgccg ggccggtcgt gacggtggcg ttcagccacc ggtgctcgcc gtcgccgacg
     7981 tcctcccagg agtcgccgtc ccacacccgg tcgtccgggt cgaggatctc gacgtcgacc
     8041 tcggcgggag cggaggtggc gtcggcggcg gtcccggcga tgcggtcgag gtcggcgacg
     8101 gtgctgtcgg cgtagggggt ctgcagctgg accgacgggt cgtcgtcgtc gatgcgcacg
     8161 gtgagggtgc gcaccggttc gacggtgccg tcggcgccgg tggcccggaa ccgcaggtgg
     8221 gtgaccccgg tcgcggtcac cgcgacgtcc gcccggtcgc cggcgacggt gctcggcgcg
     8281 gtggcggtgg cgccctcggc ccaccaggtc accgaggcga cgcctccggc ggcctcggcg
     8341 cgcagcgcca cgtcgaccgg accggtgttc caaccccgcg ggttggcctc gcgggtgcgg
     8401 gtcccggtgg tcagtggcgt ggtgggaacc gagccgatcg cgacgtcggt catcggggac
     8461 cggttgccgg ccgcgtcggt ggcgaacgcc cggatcgtcg tcgtgcccgc cggggcgctg
     8521 aacggtccgt cgtagggggc gaaccggccg ccggtcgaca ccagcacgtc gatggccccg
     8581 gggccgtcgt cggtggccga caccgcgtac cggcggtcct ccccggtgcc gacggcggtg
     8641 acagtcagct cgggagcggt gacgtccgcg gcggcgggac cggacaggtc gacggtcggg
     8701 gccaccggtg agtcgacctc gccgtcgccg tcggagtcga tgcgcagcac cggggtgcgc
     8761 tccggcgcca tctccaactc ggcggcgcct tcgggcacct ggacgtcggt ccagcgcatg
     8821 gcccgcgtgg ggttgtcctg ggtgccgtcg agcagctcga gctgcagggg gtcgccggtg
     8881 gcgtggaacg cgacccggta ggtgcgcgtc gacgacagcg gcagcgtggt gagtccggcg
     8941 tcctccgcgt tcgagcggaa ctgggtgacg ccggggacgg tgccggtcga gccgtcggcc
     9001 atcggggtga cgtggcggag gtcgtcggtc acggtgacgt cggagccgcc cagcagctcg
     9061 aggtagcgca gggagtgcgc ccggtccggg gccccctggc cggcggcggc gagtccggcg
     9121 ccgttcgcgg agccgccgca gctggcggtg tcctggtcct cggcccccag cggtgccgag
     9181 taggtgccgg gtccgtcctt ggccgacggg gtcgccagca gctggtcgag acgtccgagc
     9241 gccgcggggt tggtcgtcat cccggtgtgc tcggcccgcc cgttctcatc ggcggacggc
     9301 gaggtgagga cctggatctc ggcgtcgggc gcgttgaggt cacggccgcg gcccacccgg
     9361 gctgcgctca ccagcgggac tgtgccgtca ccgcagacca gagactgctc gacgacggtt
     9421 cggggttcgc agctgaagct ggacaggtcg cacaccggtc gggtgcggta cacgacggtg
     9481 ccgatcgtgt tgcgccccgc ctgcagcccg acgaagtggg tgatgtccac gtcggtgtcg
     9541 agcgagctcc agtcggcctg gccggcctgg gtctggaacg actcggcgac ggtgccgggg
     9601 gtgaaggtgg ggttctcggc gtcgagcatc tgcttgaggt gggcgaaggt gaagtcgtcg
     9661 cggtcgacgc cgtccaggtc gcggtcggcg ccgagctcgc ggaacaccgg gcgggtggcg
     9721 agctggtggt agtacggccc ggccatcagc tggtgcgcgg cggggaagct gccgacgatg
     9781 tggcggatga tgcccgccgg gccgttgacc ggaccggcgg cgaagtcgcc ggtgtagagg
     9841 acgttgacca gcttggggga gccgagccac ggcgccccga aggtgatcag ccggtcgacg
     9901 ggggcgtcat caccggcgtc gagcaggtag cgctgggcga ccaggctgcc catcgagtgg
     9961 gtgacgatgt tgacgtcgcg gtcgggccag aacctgcgca cgcagcccat gaagtcggcc
    10021 agtcgtcggc cgctgtcgcc gttgtcgagt cgccagtcgt aggcgaaggt gaacaggttc
    10081 gggctgttct gggatgtgtc gcagccggcg gtggtgtagc ggtcgaggcg ggcgtcgacc
    10141 cggtaggggc ggaacccggt cccttcgatg tggctgagca gcgtgtcgta gacaccgagg
    10201 gcgtcgtcgg cccaggagcc gattccgtag cacgtctcgt cgcgcagcgg cgcgaacgcc
    10261 gccaggttcg tcagcgctcg accggcctgg acgtcctcgg gccacaggct gaggtgcatg
    10321 cggtctgccc cgcagcggat ccactcctgg tcgccctcgt cgccgcggtc cgcggtgcgg
    10381 ggggctgtct gggagatgcg gctggcgccg gcgccgtgca cgaacacgac cgggttgacc
    10441 ggccgggcga ggaacaccga cgcggcgccc tgccagtcga cgtcgccggc gatcacgccg
    10501 tcggtgttga tgccgatcgc gctctggagg tgcacggtcg gctcgtccac gagccggtcg
    10561 aggcgcttga gcgtgccgtc gaaggcgacg ggcctcagcg cgccgtccgc catgaccatg
    10621 cccaccgcgg tgcggcgttc gttgatgtcg cgcagcagca cctcctgccc gagcggcgcc
    10681 accggggtgg ccggccccac gccgggtgcg accacccagg tgcggtagcc ctgctcgggg
    10741 gtgccggtgg cggcccgcat cccgatcgtg ccgtcatcgg cgatcacgtc cgcgatggcg
    10801 ctgacctgac cgaactggag ttggagcacc ggtacaccgg cgttccactg cacggcccgg
    10861 gtcacgggct ggttgaactc ggtccaggtc gcgaagccga cgacctggcc ggtctcgttc
    10921 atgtcgaggg cgctcagctg cgggttcgac ccgccgggct gggggtgcgc gagcagggtg
    10981 cggttgccgt cggggtggag gacggacgtg cccagcagga cgtcgccgcg gctgttgacg
    11041 gcgcggacgg tctcgacggt cggggcgccc ttgggctggg ccagccggcg ggtgaagtcc
    11101 ccgaggttgc cctcgtcgtc agggtcccac accccgaccc agccgtccgc gtcgggtgcg
    11161 gacagggtgg cgatgtggcc ggcgatcgtc ccgtcggggg cgaccgcgtc agcggtgatg
    11221 cgctcggcgc ccgccgcgta gagctcctcg aagggctggc ccggtcgggc ccggaacgcc
    11281 ttgggtcggc cttcgtggac gtagttgccg acgatggtgc cgtcgtcggc gatgccgacc
    11341 gggtcgacct gggtgactcc ggggacctcg ccgaacgcgt cggcgagctg gtagccggcc
    11401 atcggtgcgg tgaccatgcg gtcgccggcg agcgtcgcgg cgtcggcgtc gccgggggcg
    11461 tcctcggcgt cggccggcct gaacgtcgcc gacagggacc cgacggtgac cgcgccggcc
    11521 agcacgaccg ccgtcgctcc gagcgcgggt ctttgtcgtg tccgcgggcc cggtggtcgg
    11581 agccgctgcc actgcagcgg gacgagggcg gcacagccca cgaggatgat caggaacggg
    11641 agcatggaac gcctccgggt cgggtcgggc gggggtgccc gatcccgcca cactgaggcc
    11701 gccggccgtt cctgcccatc cccgcgcgtc gtgttcctgt ggaccgcgag cggtgggtcc
    11761 cggccacccc cagcaatgtg ggggtgtgct cgggtggccc gtcggtgcag agtggaaccc
    11821 atgatgagcc agctgaccgc cgagcgggtc cgccgtgaca tcgaggtcgt gtcgcgggcg
    11881 ggcctgccgc tccccgagtt cctgaccgag gtcgacgggt cgctgcgccg cgccgttccg
    11941 taccgggcga tgtgctcggc gttgttcgac cccgccaccc acctgtgcac cgccacggtg
    12001 aagttgggcg agctcgccgc cgacgaggag gccgacgcac gctgggggac catcgagtac
    12061 ggctacgaca acccgacgaa cttcgccgcg ttgtcggcgg ccgcacggcc ggcgacctcg
    12121 gcccgcctcg aggcgctgaa cgagccggac gtgctgttcc gggcccgcga gttcctccac
    12181 ccccagtacg ggttctccga cgagctgcgg tcgatcgccc gcgcggatgg tcacacctgg
    12241 ggcggcttcg ccctgcatcg tcgtgacggc gacccggact acaccgagga ggacatcgcg
    12301 ttcgtcggtg cgctctcgtc ggacctggcg gtcgggttcc gctcggcgct gctggtcgga
    12361 cgggtcggcg agctggacgc tccccgcgac ctgccggccg cgccggcggt gctgatcgtg
    12421 cacggcgacg acgtcctcgg ccagatgaac gacggcgcga gcgcctggct cgacgacctg
    12481 ggggtcaccc ccgaggacac cggcacatcg agcgtgatct cggggctggt cgcggcggcc
    12541 cgtcgactcg ccgccggcac cggcacgatg actccgaggc tgcgggtccg gggtcgaagc
    12601 gggcgctggc tcgtgctgca ctcgtcgccg ctgagcggac cgaccagctc gggcaccgac
    12661 gtggtcgtca ccatcgaaga ggcacgtccg cccgagatcg tggcgatcgt ggtcgcggcg
    12721 ttcgacctgt cgccccgtga gcgtgacgtc acccagctgg tgctgcaggg cgtcgacacc
    12781 cgcggcatcg cgaccgccct gagcatgtcg cgctacaccg tgcaggacca cctgaagtcg
    12841 gtcttcgaga aggccgacgt ccgcagccgt cgcgagctcg tgtcgcgggt gtacttcgac
    12901 cagtacgtcc cgcgcatggg ttccgacatc ggcgccgacg gctggttcgt cggcacctga
    12961 gcggaaccgt cacatccggt ggttcacatc cagtagttgg gtgcctcggc ggtgatggtc
    13021 acgtcgtggg ggtgcgcctc gcgcagaccg gccgggctca tccgcacgaa ctgcgctccg
    13081 gtctgcagct cgccgatggt gtgcgacccg cagtagccca tggccgagcg caggccgccg
    13141 atcagctggt acaggatctt ctgcgccggg cccttgtagg gcacccgacc ctcgacccct
    13201 tcgggcacga ccttctcggt ggtctcgacc tcgccctgga agtagcggtc cttggagaag
    13261 ctgcgggcgt tcatggcgcc gagcgagccc atgccccggt acgccttgta gcgctcaccc
    13321 tggctgagga cgatctcgcc gggcgtctcg tccacgccgg cgagcaggct gccgaccatg
    13381 accacatcgg cgcccgcggc gatcgccttg gcgatgtcgc cggagtagcg cacgccgcca
    13441 tcggcgatga tgccgacgcc gtgggcggcg gcgacccggg cgcactcctg caccgcggtc
    13501 agctgcggca cgccgacgcc ggcgacgatg cgggtggtgc agatcgagcc cggaccgacg
    13561 ccgaccttga ccgcgtcggc cccggcgtcg atcagcgcgg cggtcgccgc tccggtggcg
    13621 atgttgccgg cgaccacgtc gacgttcagg ttggccttga tcttgcggac cacctcgagc
    13681 acgccggcgg agtggccgtg cgcggtgtcg accaccagca ggtccacccc ggcgtcgacc
    13741 agggcctggg cccgctcgag cgcgtcgtcg ccgacgccga ccgcggccgc gcagcgcagc
    13801 cgaccctgcg ggtccttggt ggcgttcggg tacttgagct tcttgttgat gtccttgacg
    13861 gtgatgagcc cggtgaggcg gccgtcgtcg tcgaccacgg gcagcttctc gatgcggtgc
    13921 cggccgagga tcgcctgggc ctcctccagc gtggtgccct gtggcgcggt gaccaggccc
    13981 tcggcggtca tcacctggtc gacccgcttg gaggggtcgt cctcgaagcg caggtcgcgg
    14041 ttggtcagga tgccgaccag gtggtggtcg tcgtcgacga tcggcacgcc gctgatgcgg
    14101 tacttggcca tcaggtccag agcgtcggcc accaggtggt cgcggcccag gcagaccggg
    14161 tcgacgatca tccccgactc ggagcgcttg accttgtcga cctcggtgac ctggtcgtcg
    14221 atcgacaggt tgcggtggat gacgcccagg ccgccctcgc gggcgatggc gatggcgagc
    14281 cgggcctcgg tgacggtgtc catggccgcc gacatgagcg ggatggccag cacgatgttc
    14341 ggggtcaggc gggtcgaggt gtcgacctcg gcggggatga cgtcggacgg tccggggacc
    14401 aggcacacgt cgtcgaaggt gaggcccatg gagccgaagc ggtcgctggg gtcaccgatg
    14461 gcggcggaga ggccggccgg ggcggtcgac ggggaggcgg acggggcggg ggagtcgtcg
    14521 gagaggggcg ccatggcggg atgctagtcg gtcgtcgtca ggggatcgaa ggccgcggcg
    14581 ccggtaggtt gacggcatgg aacgccgacg tcgagtcgtg gtggtgggtg ccggcttcgc
    14641 cggcctggag ctggtgaagg gcctggccgc cgcgccggtg gacgtggtgc tggtcgacgc
    14701 caacaacttc cacacgttcc agccgctcct gtaccaggtc gcgaccgccg gtctcgacgg
    14761 cgacgacatc gccgctccgg tgcgggccgt cgtgcgttcc caggccaacg tcgacgtccg
    14821 tctgggccag gtggtcgaca tcgacgtcga tgaccggcga gtggtgttcc gcgacggacc
    14881 cgctctgtcc tatgaccgtc tcgtgctggc cgcgggcgcg gtcaccaaca cgtacggggt
    14941 gccgggggtc gacgagcacg gcttcgggct caagtcgctc gaggacgcgc tgcagctccg
    15001 tcagcacctg ctgcgccagt tcgagcgggc gtcggtcgac ccgtcgctgg tcgaacaggg
    15061 cgtgctcgat gtggtcatcg ccggcggcgg acccaccggc gtcgagctcg ccggtggaat
    15121 cgccgagctg taccgcaacg tcctctcgca cgacttcccg accctggacg tccgcaaggc
    15181 ccgggtcgtg atcgtcgaac cgggcgaccg ggtgctggcg ccgttccatc ccaagctgtc
    15241 ggcgcgggcc gctcgcaccc tggcccgcct gggcgtcgag ctggtcttcg gccagggcgt
    15301 gaccggcacc gacggcaagg tcgtccaact cgacgacggc tccagcgtgc cgtgctccac
    15361 gctggtgtgg accgccgggg tgaaggcgag cccgctcgcc gacgttctcg cccgtcacct
    15421 gggcgacgga accctgaccc gcggcggtcg ggtggtcgtc accccggagc tgaccgttcc
    15481 cggccacccc gagatcgccg tggtcggcga cctggccgcg tcacccgacg gctccggcgg
    15541 cgtgctgccg cagctcgccc cggtggccat ccagggcggg cggctggtgg ccgccaacct
    15601 ggtcgccgag ctggccgggc atcccgccga gccgttccac tacgtggaca aggggacgat
    15661 ggccaccgtc ggccgtcgca acgcggtggc gcagctgccg gggcgcctcc ggttctcggg
    15721 cacgccgggg tggctggcgt ggctgggcct gcacctgatc atgctgatcg ggttccgcaa
    15781 ccgcgccaac gtgatggtca actgggcgtg gaactacgtc acctacgacc gggcgtcgcg
    15841 actgatcgtc ggcgacgacg accacccggc ccggccgtcg ggtcgcccga tgccgaccga
    15901 tcccgaccgg tagggcgcgg gtggggccgc cgtccgcacc ggacgggccg gtagcgtggg
    15961 tccatgccga gcgtgacctt cgaaggtgag acccacggtg agatcgtcgt caaggtccgg
    16021 cgctggctgg cgtcggtcga cggccaggag gagggcgggc tcaccgcggc ggaggccgtc
    16081 acccagggcg ccgagctgac caaggacgcg ctgcggatca tcgcttcggc cgcgcccgag
    16141 cccgtggcgc agtcggacct ggtcaagggg ctgaccaaca tgggctacaa ggccaccgac
    16201 gccacccgct ccgcggtgat cgccgggttg gactcgatcg aggagatgac cgggggtggg
    16261 gtcgtgcacc aggcctccga cgtcggccgg cgggtggtgt ggcagatgaa cacctcgatg
    16321 gccaagcagc tcctgcgctc ccttcgcagc tgacggcgtg acccggggcc ctgccgtcgt
    16381 cgaccggtga gcgtcggagg cgacctgggc aggggtcggt cggcccgacc gcagtaagct
    16441 cccgcctttc ctccggacag gtgtgacgtg gcagagatcg agatcggatt gggcaaggac
    16501 gggcggcggg gctactcgct ggacgaggtg gccatcgtcc ccagccgccg gacccgcgac
    16561 gcggacgagg tcgagacgtc ctggcagatc gacgcctacc agttcgccct cccggtgatc
    16621 gcggcgccgc tcgacggggt gaccagcccg gccaccgcgg tcgagatgga ccgcttgggc
    16681 ggcgtgggcg cgctgcacgc cgaggggctg tggtgtcgtc acgacgaccc gaccgcgctg
    16741 ttggagcgca tcgccgcgca cgatccccgc gacggcgagg gcgacgaccg cgacggggtg
    16801 gcactgctgc gcgaggcgta cgacgcgccg gtgcgtcccg agctggtcga gcagcgcatc
    16861 gccgagatcc gcgacgccgg cgccacggtg tgcgtggcgg tcagcccggc gcacaccgag
    16921 gccctgctgc ccacgatcaa gaaggccgaa cccgatctgc tgatgatcca gggcacggtc
    16981 acctcggccg agcacgtcag cggcggcgcc cacgatccgc tcgacctcaa gcacctggtg
    17041 cgtcggctcg aggtgccggt ggtggtcggc gggtgcgcca gctaccaggc ggccctgcac
    17101 ctgatgcgca ccggcgcggc cgcggtgctg gtcggcatcg gcaccggcgc cacctcgacc
    17161 acgggtcgcg tgttgggcgt cggcaacccg caagccaccg cggtcgcgga cgcccgcgcg
    17221 gcgcgcatgc gtcacctcga cgagaccggc gtgtacgtgc acctgatcgc cgatggcggg
    17281 atggccaccg gcggtgacat cgccaaggcg gtcgtgtgcg gcgccgacgc ggtcatgctc
    17341 ggcgcgccgt tggccgcggc ggacgaggcg ccggccggtg gcgcggtgtg gtcgatgacg
    17401 tcgttccact cccgactgcc ccggggtcgt ctgcgacggg tgccgcggcg cgggtcgttg
    17461 gagcagatcc tgctgggccc gtcggatcgt gccgacggtc gcaccaacct gatgggcggg
    17521 ctgcgcaagg cgatggccgt gaccggccat ggcacgttga aggacctgca gaaggccgag
    17581 ctggtcgtca ccgccccgcc cgccgtgagg cccggagccc agcgatgagc ggcgacccga
    17641 tggggcagag cccagcgatg agcggcgacc cggcaggaca gagcccagcg atgagcggcg
    17701 acccggcagg acagagccca gcgatgagcg gcgacgcggt gaaggcggcg gtggtcgacg
    17761 accagccgac ggtcctggtg gtcgacttcg gcgcccagta cgcgcagctg atcgcccgcc
    17821 gagtccgcg
//
LOCUS       DAOQOD010000050        17796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1115_length_17796_cov_12.852207, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000050 DAOQOD010000000
VERSION     DAOQOD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17796
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1115_length_17796_cov_12.852207"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..551
                     /gene="nuoB"
                     /locus_tag="PLZ72_06785"
     CDS             66..551
                     /gene="nuoB"
                     /locus_tag="PLZ72_06785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNA36039.1"
                     /translation="MPKPLTKLLNLSRSYSMWVYQWGLACCAIEMGAAFASPRYDVMR
                     LGVIPLPAGPRQTDLVVISGTVTDKMAPSILRLYEQMPDPKYVISMGSCANCGGPYWD
                     SYSVTKGVDQILPVDVYVPGCPPRPEALLEGVVLLQQRIKTEDMAERWTGEAIVVGSD
                     G"
     gene            544..1302
                     /locus_tag="PLZ72_06790"
     CDS             544..1302
                     /locus_tag="PLZ72_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNA36040.1"
                     /translation="MADDAATTDAPAETPEEEVEAVQPDELREGIVARFGDLLGDALV
                     DSLIDPGVDLTIRVRTDAWRAAADVARNHLGARYFCFLSAIDWLPSPYGRSMDSEVDT
                     VLAAQAGDVAEPRSSEIVQGTTGGDTRFQVFARVAHLNGDGGFWGITIKADVPDDSLT
                     VESWVPIYAGADWHEREAWEMFGLTFTGHPGLRNIYLPSGFEGHPLRKDYPLLSRMVK
                     PWPGIVDVEPMPTDDEDGDGGGAAAEGSAEGGDS"
     gene            1302..2474
                     /locus_tag="PLZ72_06795"
     CDS             1302..2474
                     /locus_tag="PLZ72_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HNA36041.1"
                     /translation="MTAVTDRRQLQQIAARAADARVNVELETEGMTLNIGPQHPATHG
                     TLRIVARLDGEQVIAAEPVAGYMHRGYEKLSEVRTYAQATALMNRIDWLGSFANEVPF
                     ILAAERLMDVEAPPRATHIRTILFEMSRIAHLILFLGDMGVQLGAVTPVFFAFRDREF
                     VLNQIEAATGGRFHPYFDRIGGLKDDLPKGWIADTKAVMEKVRTFCDEIEDVLMGAEI
                     FETRTRGIGVIPPDVAQQYGLSGANLRASGVDWDLRRDNCVGLAYPELDWKVWTHPDG
                     DSFARFWVRLQEVRESTKIIDQLCDGLPSGPIMAKVPRIIKVPAGEAWVSTENPLGEM
                     GYYVVSQGDLTPFRVKIRSASFNNVSITPWLLRGVYVPDIITILASLYFILGDIDR"
     gene            2471..3511
                     /locus_tag="PLZ72_06800"
     CDS             2471..3511
                     /locus_tag="PLZ72_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNA36042.1"
                     /translation="MTGVLSAIELAYWQQTTIRLLAGLVAVLLLTGGVVYLYLFKFVS
                     FMQSRLGPMEAGPYGSLQLLAEVGKWIQKEDISTKDADKFIFGLAPAVVLVSTFLMFV
                     VIPAGPNAVMADLGTGIYYVLAISSLSVIGILMAGWASANKYALMGALRAGGQLIAYE
                     LPLVLGVLGVVIQAGSLNLQDIVYAQSNGEIFGISAIGNPYILTQFVAFAIFMIAVQA
                     ELTQTPFDMPIAESELVAGYQVEYSGIRFLVYFIAEFATAVGFAAIASTLFLGGWALP
                     ASWGFSDTAMHVIGPINVSIKAVFLVGVVFWVRFTFPRFREDQLQRLAWKGLIPLALA
                     NIVVTAILKVVF"
     gene            3511..4062
                     /locus_tag="PLZ72_06805"
     CDS             3511..4062
                     /locus_tag="PLZ72_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNA36043.1"
                     /translation="MPAKPSKPQVPGLIKGLGVTLRTAKETIFPDGMLKPPAPSKGAA
                     TVQYPREKEAPVTRARGVIALKEENCTACMLCVRECPDWCIYIEAHKTLAPPRREGGK
                     PRVVNELDRFDFDYALCMYCGICVEVCPFEALFWSPEYEYSEAHISDLIHDRSRLAQW
                     METVPDFEPYESGSEQKVKKVPR"
     gene            4148..4696
                     /locus_tag="PLZ72_06810"
     CDS             4148..4696
                     /locus_tag="PLZ72_06810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNA36044.1"
                     /translation="MSLFATAQSAVSFDTPANIAFAVIAVVMVIAAVRVVTTRNVVHA
                     ALWLMIVLGGNGLLYLLLQAEFVAMTQFLVYLGAIVVLFLFGIMLTRAPLGRSDDLDN
                     DQRWMGVAVSLILLVVIGYSLIKTYSDDKLTFGAVRNAGTNAQTQDLATSIFVDYLVP
                     FEVVSVLLLAALIGAIVLARRD"
     gene            4698..4994
                     /gene="nuoK"
                     /locus_tag="PLZ72_06815"
     CDS             4698..4994
                     /gene="nuoK"
                     /locus_tag="PLZ72_06815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNA36045.1"
                     /translation="MLLNQFLLLAAVLFAVGVYGVIVRRNGVLVLMSIELILNAVNIN
                     LIAFSTFWGMGGQVFALFVIAVAAAEVGVGLAIVLLIYRNKRSIDIDALDELKG"
     gene            5006..7348
                     /locus_tag="PLZ72_06820"
     CDS             5006..7348
                     /locus_tag="PLZ72_06820"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNA36046.1"
                     /translation="MWFFTHVWIVPAMMALSFLLILFFGKKMPKKGAEIGIFFVALCL
                     IYSLFTLGNWITWSHDSPTLAEGAPEAQQLAAEKGALANVPAECSKVAAHPVASVASE
                     KAEGGSGDSGTSGGHGDEGATAANSTGTGTGEVASGAAATSGSAKAAEAGAEETLTRP
                     VVRCATWIDSPEASITWGTQIDGLSIMMLLVVPLISLLVHIFSTDYVHGDRRYTHYFA
                     FLSLFTASMLFFVLSQSTLQMIVGWELVGVCSFALIGHWWEEKPNSDAALKAFLTNRV
                     GDMGLLIGMIILFFAAGNSFSIMDITILANEGGIRHTVLMAASICLIAAVMSKSGQFI
                     LHTWLPDAMAGPTPVSALIHAATMVVAGIFMVARLYPVFFHGLSISGSSINLLAVVGA
                     VTALFGALLAFVQKDIKKVLAYSTVSQLGFMVTSLGVGAWTAAMFHLFTHAFFKACLF
                     LGAGSLSHACHHSFNMVDDMGGLRKIMPKTFWTYLIATGALAGIFPLSGFWSKDEILA
                     GTGSFPGSSHANGTYHLMLIMLLIAAFCTAAYMTRTIWYAFYGEYRGHGHPHESGPRI
                     TVPLIILATLGAIAGLANLPAQLSGWLDVPDSVQTRFEHLVEPVGLYFPQITHAEFNP
                     ALAILSVAVGGAGIVLVYLYYWKGAFAGLHGLAGRNRTAAFFKNILVNKYYFDWLYTD
                     VITGWVKGGLARGSNWVNKNVIDGVVNGVGRGAAAGGRWVYDNVDQTVVDGAVNGAGS
                     VSSATGGGLRRITSGHIQQYAALFFAAAAVLAGMFVVLIG"
     gene            7374..8924
                     /locus_tag="PLZ72_06825"
     CDS             7374..8924
                     /locus_tag="PLZ72_06825"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNA36047.1"
                     /translation="MKQFLNDWGLILVTFLPMVGVLAMLCVPRESEETHKLLAVITSL
                     ATAFVGILLMLNFNYDRAGILQFRVDHSWIDAINSRFIMGLDGLSLPLLALTLLIMPL
                     VFIYSWNHIPEPGNVKAFFVLMLVLEMGMIGTFLAEDLILFFVFFEIVLLPMYFLIGV
                     WGGPERKYASIKFFIYTLLGSALMLVAFVAVYFLVKNPVTGEAIQTFSIPELKAAGGE
                     GITRAAQVVIFGGMFMGFGIKVPMFPFHTWLPDAHTQAPTQGSVILAAVLLKLGTYGF
                     MRIAIPILPDGARQWAPVIGVLAVIGIIYGALCCLAQTDMKRLIAFSSVSHMGFTMLA
                     ISTLTTFGFNAAVFGMVAHGLITGMLFFIAGSMKDRYHTLEIKRLGGLLVSAPKLGWI
                     FGLCTMASLGLPGLAGFWGEFPAILSAYAPAEGLNITLFRGLMIAAALGTVLAAGYLL
                     WLYQRTAFGTPTEEFADDPHIHDVTTPEWISWTPLLVLIVLLGFVPAIIFNVTEPAVN
                     IIAKTVGG"
     gene            8931..10457
                     /locus_tag="PLZ72_06830"
     CDS             8931..10457
                     /locus_tag="PLZ72_06830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNA36048.1"
                     /translation="MSLAAQLVAQVGAPVSFRGPTIDYHALAPEIVLSAAVVVLVLLD
                     SIKLERAKAYMPVVTNLALLGALIPLLTLAYDGGTRALFGGSYVVGPMPLMLKAMFIV
                     TTYVVVLLSTNYMAEGDYWESEYYTLLVSSLLGMVVMASSRDLIGIFVALELLSIPAY
                     MLASWRKGDVRSNEAGLKYFLMGVFASAVLLYGMSLVYGLGGSTRLEDIGRVLADAPT
                     DGVPVVTLALVFVVIGFAFKVSAVPFHVWAPDTYEGAPTPVTTFLAVASKAAGFVALI
                     SLVFYAFLPRADIIEPLFFVLAIGSMTVGNLAALRQTNVVRMLAYSGIAHAGFMLAPF
                     VVAGSNPFRSMQSIVVYLVIYAAMNLGAFACVIAMARQTRSAELKDLAGVVRSMPGLA
                     WCMVLFLASLIGMPPLGGWYAKFGVFKVVVGAETGLGYATAIIMAINTAIAAYYYLNV
                     AKTMIFDDPPEGAPVRAPRTPPSLAAAIAISLVATFAFGILPGLLTNVTSYGPLALGG
                     "
     gene            10545..11729
                     /locus_tag="PLZ72_06835"
     CDS             10545..11729
                     /locus_tag="PLZ72_06835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNA36049.1"
                     /translation="MKSLADRVARFGSVRFDEFVDAALYDPDDGFFTSMGDAGRGRAG
                     RAGGDFITSPEVGPLFGAVLARFIDARWEELDRPDPFVVIEAGAGRGTLALSVLAAEP
                     VCAPALRYVLVEHSAELRRAQARHLALADPAEVLGPPGDPDDGVPSPAAGSGPLVCSL
                     VELPVAVVDGVVIANELLDNIPFRLFECTESVPDGEAWAEVRVTVDAGRFVELRVPAE
                     PEISARLDVLAPDARPGSRVPLQDGASAWLHAALGVLNRGSVLVFDYAAASTASLADR
                     PVDEWLRTYRDHDRGVGPLEDPGTQDVTVEVAVDQLAMIAAPDSDLAQADWLRRWGIQ
                     GMVEEGNRLWAERAGVGDLAALRARSRAVEAEALCDPTGLGAFRTLEWRRPATAPPPP
                     LS"
     gene            11894..13906
                     /gene="acs"
                     /locus_tag="PLZ72_06840"
     CDS             11894..13906
                     /gene="acs"
                     /locus_tag="PLZ72_06840"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNA36050.1"
                     /translation="MNDAATPDDGPTIEDYYREDRTFPPSPEFTAQAVLGDPSIYDRA
                     EADPEAFWAEQARDLITWDTDFHTTLEWELPFAKWFVGGTLNVSANCLDRHVAAGRGD
                     KVAIHWEGEPGDTRAITYAELLADVSRFANVLKSLGIGTGDRVCIYLPMIPEAVVAML
                     ACARLGAAHSVVFGGFSPDSLIDRIDDAGATVVVTSDAGYRRGKPFLLKPNVDVAVAA
                     CPTVRSVVVVDRCHADVPMEDGRDHWWHNLMAEQSDHCPPVSVDAEQLLFLLYTSGTT
                     ARPKGIMHTSGGYLTQVAWTHKVTFDLHADTDVYWCAADIGWVTGHSYIVYGPLANGA
                     TTVMYEGTPDTPREELRNGTDPAGWDKDRLWDIIERYGVTQLYTAPTAIRTFMKWGAE
                     WPERHDLSSLRVLGTVGEPINPEAWMWYHRHIGSERCPIVDTWWQTETGSQMVNPLPG
                     LTATKPGSACRPLPGIGAELVDDDGNVVTRGGGYLTLTRPWPSMLRGIWGDPERYRDT
                     YWSRFEGRYFAGDGAKLDEDGYLWVLGRVDDVMNVSGHRISTAEVESALVDHPAVAEA
                     AVVGATDATTGQAIVGYVILRGSYTASEELREEIRQHVSTKLGAIARPKAVILVTDLP
                     KTRSGKIMRRLLRDVVEGRELGDTTTLADATVVEAIREGAATSTED"
     gene            13954..14451
                     /locus_tag="PLZ72_06845"
     CDS             13954..14451
                     /locus_tag="PLZ72_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36051.1"
                     /translation="MPTWDPDFSNWLWRDDPLDAATSIVFDMDGVLSNASTRQHYLEH
                     PRRDWESFFQACGDDELIDEVAQLLDVLDTELQIVLLTARPIRVQPQTLAWLERYGLR
                     WDVLIMREYGDYMAARHFKQRTVYELRHRGHELQLAFEDDPRNVAMFHGEGVPCIYIH
                     SGYYN"
     gene            complement(14568..15140)
                     /locus_tag="PLZ72_06850"
     CDS             complement(14568..15140)
                     /locus_tag="PLZ72_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin G domain-containing protein"
                     /protein_id="HNA36052.1"
                     /translation="MIDDGPDGLDGTIGSQVSSGVEEQGAVAQRRAAVAPDEPPVAVD
                     RLAEVPHDKRLNPGDGPYAVTVRLRTTSPKGNVVQKGQFGVPGGYFKIDMDDGRVACL
                     FLGSKGSSHARSRRSIVDGRWHEVRCERDGDDLVLRVDGAEVARRDKPVGSIDNTFPL
                     TIAGKLACDQVERFCDYFSGDIDRVLIERG"
     gene            complement(15401..16243)
                     /locus_tag="PLZ72_06855"
     CDS             complement(15401..16243)
                     /locus_tag="PLZ72_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNA36053.1"
                     /translation="MRNNLKTAVLLAGLGGLCMFIGSYWGPTGVVVGLLIGLAMCGGS
                     YWASDKLAIRSAKAIEVTAEQMPQYHAIVADLAMRAGIPKPTLYVSPNPQPNAFATGR
                     DPDHAAVCVTQGLLQILDWDEIRGVLAHELSHVRNRDILIGSVAAAVAMGITFVASMA
                     RFAMIFGGGRSDERSNPFGALLMVILAPIAAALIQMAISRSREYEADASAARMIGTGE
                     PLARALEKLEAGAERVPVKVNPAEASAYIVNPLRGRNASNLFMTHPPVPERIARLRSG
                     AWAV"
     gene            complement(16318..16806)
                     /locus_tag="PLZ72_06860"
     CDS             complement(16318..16806)
                     /locus_tag="PLZ72_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNA36054.1"
                     /translation="MPSFDITSEVDAQEIRNAVDQASREVANRFDFKDTGSSLDLGDD
                     AITLRSSTEDRLAALRQVLEEKFVKRGISMKALSYEKVEEASGATVRQVAKLVAGINS
                     DRAKDLNKFIKALGLKGVQSQTQGEQVRVTAKKRDDLQAVIAACKDHDFEIPLQFGNF
                     RD"
     gene            16908..16993
                     /locus_tag="PLZ72_06865"
     tRNA            16908..16993
                     /locus_tag="PLZ72_06865"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16942..16944,aa:Tyr,seq:gta)
     gene            complement(17049..17546)
                     /locus_tag="PLZ72_06870"
     CDS             complement(17049..17546)
                     /locus_tag="PLZ72_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNA36055.1"
                     /translation="MILRPAVAADAPAILEIYNHEVLTSNVTFDLTPRTLEDQRRWIT
                     DRSGAHAAVVAVDADDGSEVVIGFGALSPYRDRPGYSTSVEDSVYVHDAHRGRGVGVA
                     LLSELVERATAHGFHAVFARIVAGHDASIGLHARLGFEIVGREKEVGRKFGRFHDVVV
                     MERLL"
BASE COUNT         2585 a         6456 c         5781 g         2974 t
ORIGIN      
        1 ggactcgtgt acgcctggcg caagggggtg ctcaagtggg cctagggctc gtcgagagcg
       61 gcaagatgcc caagccgctc accaagctcc tcaacctgag ccgcagctac tccatgtggg
      121 tgtaccagtg gggtctcgcg tgctgcgcga tcgagatggg cgccgcgttc gcctctcccc
      181 gctacgacgt gatgcgcctc ggcgtcatcc ctctgcccgc cgggccccgt cagaccgacc
      241 tcgtggtcat ctccggcacc gtcaccgaca agatggctcc gtccatcctc cgtctgtacg
      301 agcagatgcc cgatcccaag tacgtgatct cgatggggtc ctgcgccaac tgcggcggcc
      361 cctactggga cagctactcg gtgaccaagg gcgtcgacca gatcctgccc gtcgacgtgt
      421 acgtgccggg ctgtccgccc cggcccgaag cgctgctcga gggcgtcgtg ctgctccagc
      481 agcgcatcaa gaccgaggac atggccgagc gctggaccgg cgaggcgatc gtggtgggct
      541 ccgatggctg acgacgccgc caccaccgac gcccccgccg agacccccga ggaagaggtc
      601 gaagcggtcc agcccgacga gctgcgcgag ggcatcgtcg cccgcttcgg cgacctgctc
      661 ggtgacgccc tcgtcgactc gctgatcgac cccggcgtcg atctgaccat ccgggtccgc
      721 accgacgcct ggcgcgccgc cgccgacgtg gcccgcaacc acctcggcgc ccgctacttc
      781 tgcttcctgt cggccatcga ctggctgccg tcgccctacg gccggtccat ggactccgag
      841 gtcgacaccg tcctcgccgc ccaggccgga gacgtcgccg agccccgctc gagtgagatc
      901 gtgcagggca ccaccggcgg cgacacccgc ttccaggtgt tcgcccgcgt cgctcacctc
      961 aacggcgacg gcggcttctg gggcatcacc atcaaggccg acgtccccga cgactccctg
     1021 accgtcgagt cgtgggtgcc gatctacgcc ggtgccgact ggcacgagcg cgaggcgtgg
     1081 gagatgttcg gcctcacctt caccggccac cccggcctgc gcaacatcta cctgccgagc
     1141 ggcttcgagg gtcacccgct gcgcaaggac tacccgctgc tcagccgcat ggtgaagccg
     1201 tggcccggca tcgtcgacgt cgagcccatg cccaccgacg acgaagacgg cgacggcggc
     1261 ggtgctgccg cggagggttc cgccgaggga ggagacagct gatgaccgcc gtcaccgacc
     1321 gccgccagct ccagcagatc gcggcccgcg ccgcggacgc ccgggtcaac gtcgagctcg
     1381 agaccgaggg catgaccctc aacatcggcc cgcagcaccc ggccacccac ggcacgctgc
     1441 gcatcgtcgc ccgcctcgac ggcgagcagg tcatcgccgc ggagccggtc gccggctaca
     1501 tgcaccgcgg ctacgagaag ctctccgagg tccgcaccta cgcgcaggcc accgcgctga
     1561 tgaaccggat cgactggctc ggcagcttcg ccaacgaggt cccgttcatc ctcgccgccg
     1621 agcggctgat ggacgtcgag gccccgcccc gcgccaccca catccgcacg atcctcttcg
     1681 agatgtcgcg gatcgcgcac ctgatcctgt tcctcggcga catgggcgtg cagctcggcg
     1741 ccgtcacccc ggtgttcttc gccttccgcg accgcgagtt cgtcctcaac cagatcgagg
     1801 cggccaccgg cggacggttc cacccgtact tcgaccgcat cggcggcctc aaggacgacc
     1861 tgcccaaggg ctggatcgcc gacaccaagg ccgtcatgga gaaggtccgc accttctgcg
     1921 acgaaatcga agacgtcctg atgggcgccg agatcttcga gacccgcacc cgcggcatcg
     1981 gcgtcatccc ccccgacgtc gcccagcagt acgggctgtc gggcgccaac ctgcgcgcct
     2041 cgggcgtcga ctgggacctg cgccgcgaca actgcgtcgg cctcgcctac cccgagctcg
     2101 actggaaggt ctggacccac cccgacggcg acagcttcgc ccgcttctgg gtgcgcctcc
     2161 aggaggtgcg cgagtccacc aagatcatcg accagctctg cgacggcctg cccagcgggc
     2221 cgatcatggc caaggtgccc cgcatcatca aggtgcccgc cggagaagcg tgggtctcga
     2281 ccgagaaccc gctcggcgag atgggctact acgtggtcag ccagggcgac ctgacgccgt
     2341 tccgggtcaa gatccgctcc gccagcttca acaacgtgtc gatcacgccg tggctgctgc
     2401 gcggcgtgta cgtccccgac atcatcacga tcctggcgtc gctctacttc atcctggggg
     2461 acatcgaccg atgaccggtg tcctgtccgc gatcgagctc gcctactggc agcagacgac
     2521 catccgcctg ctcgccgggc tggtcgccgt gctgctgctg accggcggcg tcgtctacct
     2581 gtacctgttc aagttcgtct ccttcatgca gagccggctc gggcccatgg aagccggccc
     2641 ctacggctcg ctccagctgc tcgccgaggt cggcaagtgg atccagaagg aagacatctc
     2701 caccaaggac gccgacaagt tcatcttcgg cctggccccc gcggtggtgc tggtgtcgac
     2761 gttcctgatg ttcgtcgtca tccccgccgg ccccaacgcc gtgatggccg acctgggcac
     2821 cggcatctac tacgtgctgg ccatcagctc gctgtcggtc atcggcatcc tcatggccgg
     2881 ctgggcgtcg gccaacaagt acgccctcat gggcgccctg cgcgccggcg gccagctcat
     2941 cgcgtacgag ctcccgctgg tgctcggcgt gctcggcgtg gtcatccagg ccggcagcct
     3001 caacctccag gacatcgtct acgcccagtc caacggcgag atcttcggca tctccgccat
     3061 cggcaacccg tacatcctca cccagttcgt cgccttcgcc atcttcatga tcgccgtgca
     3121 ggccgagctg acccagacgc cgttcgacat gccgatcgcc gagtccgagc tggtcgcggg
     3181 ctaccaggtc gagtactccg gcatccgctt cctcgtctac ttcatcgccg agttcgccac
     3241 cgccgtcggc ttcgccgcca tcgcctccac cctgttcctc ggcggctggg cgctgccggc
     3301 gtcgtggggc ttctccgaca ccgccatgca cgtcatcggc ccgatcaacg tgtcgatcaa
     3361 ggccgtgttc ctggtcggcg tcgtgttctg ggtgcgattc accttccccc gcttccgtga
     3421 ggaccagctc cagcgcctgg cgtggaaggg gctcatcccg ttggcgctcg ccaacatcgt
     3481 ggtgacggcg atcctgaagg tggtgttctg atgcccgcca agccgagcaa gccgcaggtg
     3541 ccgggtctca tcaagggcct cggcgtcacc ctccgcaccg ccaaggagac gatcttcccc
     3601 gacggcatgc tcaagccgcc ggctccgtcc aagggcgcgg ccaccgtgca gtacccgagg
     3661 gagaaggagg cgccggtcac ccgggcccgc ggcgtgatcg ccctcaagga ggagaactgc
     3721 accgcctgca tgctgtgcgt gcgcgagtgc cccgactggt gcatctacat cgaggcgcac
     3781 aagaccctcg ccccgccgcg gcgtgagggc ggcaagcccc gcgtggtcaa cgagctcgac
     3841 cgcttcgact tcgactacgc gctgtgcatg tactgcggca tctgcgtcga ggtctgcccg
     3901 ttcgaggcgc tgttctggag tcccgagtac gagtactccg aggcccacat ctcggacctg
     3961 atccacgacc ggtcccgcct ggcgcagtgg atggagaccg tgcccgactt cgagccctac
     4021 gagtccggct cggagcagaa ggtcaagaag gtgccgcgat gagtgagcgc agccagcgat
     4081 cgcggcggcg cgaggcacga gcccgccgcg gatggggtcc gggggcgcag ccccaggagg
     4141 tgagggcatg agcctgttcg ctacggcgca gtccgccgtc tccttcgaca cccccgccaa
     4201 catcgccttc gcggtgatcg cggtggtgat ggtgatcgcc gcggtccgcg tcgtcaccac
     4261 ccgcaacgtc gtgcacgccg cgctgtggct gatgatcgtg ctcggcggca acgggctgct
     4321 ctacctgctg ctgcaggccg agttcgtcgc catgacccag ttcctggtct acctgggcgc
     4381 catcgtcgtg ctgttcctgt tcggcatcat gttgacccgg gcgccgctcg gccgctccga
     4441 cgacctcgac aacgaccagc gctggatggg cgtggcggtg tcgctgatcc tgctcgtggt
     4501 catcggctac tcgctgatca agacctactc cgacgacaag ctcaccttcg gggcggtgcg
     4561 caacgccggc accaacgccc agacccagga cctggccacg tcgatcttcg tcgactacct
     4621 cgtcccgttc gaggtcgtgt cggtcctcct gctcgcggcg ctgatcgggg ccatcgtgct
     4681 ggcccggagg gactgacgtg ctgctcaacc agttcctgct gctcgccgcc gtgctgttcg
     4741 ccgtcggcgt ctacggcgtc atcgtgcggc gcaacggcgt gctcgtgctg atgtcgatcg
     4801 agctcatcct caacgccgtg aacatcaacc tgatcgcctt ctccaccttc tggggcatgg
     4861 gcgggcaggt gttcgccctg ttcgtgatcg ccgtcgccgc cgccgaggtc ggcgtcgggt
     4921 tggcgatcgt gctgctcatc tatcggaaca agcgctccat cgacatcgac gcgctcgacg
     4981 agttgaaggg ctgacaccgc gcatcatgtg gttcttcacc cacgtctgga tcgtgccggc
     5041 catgatggcc ctgtcgttcc tgctgatcct gttcttcggc aagaagatgc ccaagaaggg
     5101 tgccgagatc ggcatcttct tcgtggcgct ctgcctgatc tactcgctgt tcaccctcgg
     5161 caactggatc acctggtccc acgactcgcc gacgctggcc gagggtgccc ccgaggccca
     5221 gcagctcgcg gccgagaagg gcgcgctcgc caacgtcccc gccgaatgct ccaaggtcgc
     5281 cgcgcacccg gtggcctcgg tcgcctccga gaaggccgag ggcggttccg gagacagcgg
     5341 cacgagcggc ggccacggcg acgagggcgc caccgcagcc aacagcaccg gcaccggcac
     5401 cggtgaggtc gcctccggcg cagccgccac ctcgggctcc gccaaggccg ccgaggccgg
     5461 cgccgaagag acgctgaccc ggccggtcgt gcgctgcgcc acctggatcg actcccccga
     5521 ggcgtcgatc acctggggca cccagatcga cggcctgtcg atcatgatgc tgctggtcgt
     5581 gccgctgatc tcgctgctgg tgcacatctt ctcgaccgac tacgtgcacg gcgaccggcg
     5641 ctacacccac tacttcgcct tcctgtcgct gttcaccgcc tcgatgctgt tcttcgtgct
     5701 gtcccagagc acgctgcaga tgatcgtcgg ctgggagctc gtcggtgtct gctcgttcgc
     5761 gctgatcggg cactggtggg aggagaaacc caactccgac gccgcgctca aggcgttcct
     5821 caccaaccgc gtcggcgaca tgggcctgtt gatcgggatg atcatcctgt tcttcgcggc
     5881 cgggaactcg ttctcgatca tggacatcac gatcctggcc aacgaaggcg ggatccgaca
     5941 caccgtgctg atggcggcca gcatctgcct gatcgccgcg gtcatgtcca agtcgggcca
     6001 gttcatcctg cacacctggc tgcccgacgc catggccggc cccaccccgg tgtccgcgct
     6061 catccacgcc gcgaccatgg tcgtcgccgg catcttcatg gtcgcccggc tgtacccggt
     6121 gttcttccac ggcctgtcga tcagcggctc ctcgatcaac ctgctcgccg tcgtcggcgc
     6181 cgtcaccgcc ctgttcggag cgcttctcgc cttcgtccag aaggacatca agaaggtgct
     6241 ggcctactcg acggtcagcc agctcggctt catggtcacc tcgctcggcg tcggggcgtg
     6301 gaccgcggcc atgttccacc tgttcaccca cgccttcttc aaggcctgcc tgttcctggg
     6361 tgccggctcg ctcagccacg cgtgccacca cagcttcaac atggtcgacg acatgggtgg
     6421 tctgcggaag atcatgccca agacgttctg gacctacctg atcgccaccg gcgccctcgc
     6481 aggcatcttc ccgctgtcgg ggttctggtc caaggacgag atcctggccg gaaccggttc
     6541 gttcccgggc agcagccacg ccaacggcac ctaccacctg atgctgatca tgttgctgat
     6601 cgcggcgttc tgcaccgcgg cctacatgac ccgcaccatc tggtacgcgt tctacggcga
     6661 gtaccgcggc cacggacacc cccacgagtc cggcccgcgc atcaccgtgc cgctcatcat
     6721 cctcgccacc ctcggcgcca tcgccggtct ggccaacctg ccggcgcagc tctcgggctg
     6781 gctcgacgtc cccgactcgg tgcagacccg cttcgagcac ctggtcgagc cggtcgggct
     6841 gtacttcccg cagatcaccc acgccgagtt caacccggcg ctcgccatcc tgtcggtcgc
     6901 ggtcggcggc gccgggatcg tgctcgtcta cctctactac tggaagggcg cgttcgccgg
     6961 cctgcacggc ctggccggac gcaaccgcac cgcggcgttc ttcaagaaca tcctggtcaa
     7021 caagtactac ttcgactggc tgtacaccga cgtcatcacc ggctgggtca agggcgggct
     7081 cgcccgcggc agcaactggg tgaacaagaa cgtcatcgac ggcgtcgtca acggtgtcgg
     7141 ccgcggcgcc gccgcgggcg gacggtgggt ctacgacaac gtcgaccaga ccgtggtcga
     7201 cggggcggtc aacggtgccg gttcggtctc gagcgccacc ggcggtggcc tccgccgcat
     7261 caccagcggt cacatccagc agtacgcagc cctgttcttc gcggctgccg cagtcctggc
     7321 cggcatgttc gtcgtgctca tcggctgatc cgagtaggga gaaccacgtt ccgatgaaac
     7381 agttcctgaa cgactggggc ctgatcctcg tcacgttcct gccgatggtg ggcgtgttgg
     7441 cgatgctgtg cgtccccagg gagtccgagg agacgcacaa gctgctggcc gtgatcacgt
     7501 cgctggccac ggcgttcgtc ggcatcctgc tgatgctgaa cttcaactac gaccgggccg
     7561 ggatcctcca gttccgggtc gatcacagct ggatcgacgc catcaacagc cgcttcatca
     7621 tgggtctcga cgggctctcc ctcccgctgt tggccctgac gctgctgatc atgccgctgg
     7681 tgttcatcta cagctggaac cacatccccg agcccggcaa cgtcaaggcg ttcttcgtcc
     7741 tgatgctcgt gctcgagatg ggcatgatcg gcacgttcct ggccgaggac ctcatcctct
     7801 tcttcgtctt cttcgagatc gtgctgctgc cgatgtactt cctcatcggc gtgtggggcg
     7861 gccccgagcg caagtacgcg tcgatcaagt tcttcatcta cacgctgctc ggctcggcgc
     7921 tcatgctggt cgccttcgtc gccgtctact tcctggtcaa gaacccggtg accggcgagg
     7981 ccatccagac cttctccatc cccgagctca aggccgccgg cggcgagggc atcacccggg
     8041 ccgcccaggt cgtcatcttc ggcggcatgt tcatgggctt cggcatcaag gtcccgatgt
     8101 tcccgttcca cacctggctg cccgacgccc acacccaggc ccccacgcag ggctcggtga
     8161 tcctggccgc cgtgctgctg aagctcggca cctacgggtt catgcgcatc gccatcccga
     8221 tcctgcccga cggcgcccgc cagtgggccc cggtcatcgg cgtcctcgcc gtcatcggca
     8281 tcatctacgg cgccctgtgc tgtctggccc agaccgacat gaagcgcctg atcgccttct
     8341 cgtcggtcag ccacatgggc ttcaccatgc tggccatctc caccctcacc accttcggct
     8401 tcaacgccgc ggtgttcggg atggtcgccc acggcctcat caccggcatg ttgttcttca
     8461 tcgccggctc catgaaggac cgctaccaca ccctcgagat caagcgcctc ggcggcctgc
     8521 tggtctcggc gcccaagctg ggctggatct tcggtctgtg caccatggcg tcgttgggcc
     8581 tgcccggcct ggccggcttc tggggtgagt tccccgccat cctgtcggcc tacgcgccgg
     8641 ccgagggcct caacatcacg ctcttccggg gcctgatgat cgccgccgct ctcggcacgg
     8701 tcctcgctgc gggctacctg ctgtggctgt accagcgcac cgcgttcggc acgcccaccg
     8761 aggagttcgc ggatgacccg cacatccacg acgtcacgac ccccgagtgg atctcctgga
     8821 cgccgctgct ggtcctgatc gtcctgttgg gcttcgtgcc cgccatcatc ttcaacgtga
     8881 ccgaaccggc ggtgaacatc atcgccaaga cggtcggagg ctgatcgacc gtgtcgctgg
     8941 cagcccagct cgtcgcccag gtcggggcac cggtgtcgtt ccggggaccg acgatcgact
     9001 accacgctct cgcgcccgag atcgtgctca gcgccgcggt cgtggtgctg gtgctgctgg
     9061 actcgatcaa gctggagcgg gccaaggcct acatgccggt cgtcaccaac ctggcgctgc
     9121 tcggcgcgct gatcccgctg ctgaccctcg cctacgacgg cggcacccgg gcgctgttcg
     9181 gcgggtccta cgtcgtcggg cccatgccgc tgatgctcaa ggccatgttc atcgtgacca
     9241 cctacgtggt cgtgctgctg tcgaccaact acatggccga gggcgactac tgggagtccg
     9301 agtactacac gctcctggtc agctcgctgt tgggcatggt cgtcatggcc tccagccgtg
     9361 acctgatcgg catcttcgtc gccctcgagc tcctgtcgat ccccgcctac atgctggcgt
     9421 cgtggcgcaa gggcgacgtc cgctccaacg aggccggcct caagtacttc ctgatgggcg
     9481 tgttcgcctc ggcggtgctg ctctacggca tgtcgctggt gtacggcctg ggcggctcga
     9541 cccgcctgga ggacatcggc cgggtcctcg ccgatgcccc caccgacggc gtcccggtcg
     9601 tgaccctggc gctggtgttc gtggtgatcg gcttcgcctt caaggtgtcc gccgtgccgt
     9661 tccacgtgtg ggcgcccgac acctacgagg gcgcacccac cccggtgacc accttcctgg
     9721 ccgtcgcgtc caaggccgcc ggcttcgtcg cgctgatcag cctggtcttc tacgccttcc
     9781 tgccccgggc cgacatcatc gagccgctgt tcttcgtgct ggcgatcggc tcgatgaccg
     9841 tcggcaacct ggccgctctg cgccagacca acgtggtgcg catgctcgcc tactccggca
     9901 tcgcgcacgc cgggttcatg ctcgccccgt tcgtggtcgc gggctccaac ccgttccggt
     9961 cgatgcagtc gatcgtcgtg tacctggtga tctacgcggc catgaacctg ggcgccttcg
    10021 cctgtgtgat cgccatggcc cgccagaccc gctcggccga gctgaaggac ctggccggcg
    10081 tcgtgcgctc catgccgggt ctggcgtggt gcatggtgct gttcctggcc tcgctgatcg
    10141 gcatgccgcc cctcggtggc tggtacgcca agttcggcgt gttcaaggtc gtcgtcggcg
    10201 ccgagaccgg cctcggctac gccaccgcga tcatcatggc gatcaacacg gcgatcgcgg
    10261 cgtactacta cctgaacgtg gccaagacga tgatcttcga cgacccgccg gagggcgctc
    10321 cggtccgggc gccgcgcacc ccgccgtcgc tggcggcggc catcgccatc tcgttggtgg
    10381 ccacgttcgc cttcggcatc ctgccggggc tgctgaccaa cgtcacctcg tacggcccgc
    10441 tcgccctcgg cggctgacgg cccatgcccg cccccacccg ttctgttcgt tgtttcacac
    10501 ctccagagtt gtgaaacaac gaacagaacg ggggtagcgg ggcgatgaag tctctggccg
    10561 accgcgtcgc ccgcttcggg tcggtccgtt tcgacgagtt cgtcgacgcc gcgctctacg
    10621 accccgacga cgggttcttc acctcgatgg gtgacgccgg ccgaggtcgc gccggacggg
    10681 cgggaggcga cttcatcacc agccccgagg tcggccccct gttcggcgcc gtgctcgccc
    10741 gcttcatcga cgcccgctgg gaggagctcg accgacccga cccgttcgtg gtgatcgagg
    10801 ccggcgccgg acgcggcacc ctcgccctgt cggtgctggc cgcggagccg gtgtgcgcgc
    10861 cggcgctgcg ctacgtgctg gtcgagcact ccgccgagct gcgacgtgcc caggcccgtc
    10921 atctggcgtt ggccgatccg gccgaggtgc tcggcccgcc gggcgatccc gacgacgggg
    10981 tgccgtcacc ggcggcggga tcgggtccgc tcgtgtgctc gctggtcgag ctgcccgtcg
    11041 ccgtggtcga cggcgtcgtg atcgccaacg agctgctcga caacatcccg ttccggctct
    11101 tcgagtgcac cgagtccgtt ccggatggcg aggcctgggc cgaggtgcgc gtcaccgtcg
    11161 acgccggccg gttcgtcgag ctgcgcgtcc ccgccgagcc ggagatctcc gcccgactcg
    11221 acgtcctcgc ccccgacgcc cgcccgggtt cgcgggtccc gctgcaggac ggtgcctccg
    11281 catggctcca cgccgcgctc ggcgtgctga accgagggtc ggtgctggtg ttcgactacg
    11341 cggcggcgag caccgcatcg ctggcggacc gaccggtcga cgagtggctg cgcacctacc
    11401 gcgaccacga ccgcggcgtc ggtccactcg aggatccggg cacccaggac gtcaccgtcg
    11461 aggtcgccgt cgaccagctc gccatgatcg ccgcccccga ctcggacctg gcccaggccg
    11521 actggctgcg ccggtggggg atccagggca tggtcgagga gggcaaccgg ctgtgggccg
    11581 agcgggccgg tgtcggcgat ctggctgcgc tgcgggcacg cagccgtgcg gtcgaggccg
    11641 aggcgctgtg cgacccgacc ggcctcggcg ccttccgcac cctcgaatgg cgccgccccg
    11701 ccaccgcacc cccacccccg ctttcatgac catccgcgcc actggtggcg cgaacggtca
    11761 tgagaatcac gccaccgcac ctccaccccc gttttcatga ccatccgcgc cactggtggc
    11821 gcgaacggtc atgagaatcc gggggatggg acggcggtac agtccgacgg ggatcacgac
    11881 gacaggggga acgatgaacg acgcggccac gcccgacgac ggcccgacca tcgaggacta
    11941 ctaccgggag gaccggacct tcccgccgtc gccggagttc accgcccagg cggtccttgg
    12001 cgacccgtcg atctacgacc gcgccgaggc cgaccccgag gcgttctggg ccgagcaggc
    12061 ccgcgacctc atcacctggg acaccgactt ccacaccacc ctcgagtggg agctgccgtt
    12121 cgccaagtgg ttcgtcggcg gcaccctcaa cgtgagcgcc aactgcctcg accgccacgt
    12181 cgccgccggc cgcggcgaca aggtcgccat ccactgggag ggcgaaccgg gcgacacccg
    12241 cgccatcacc tacgccgagc tcctcgccga tgtcagccgg ttcgccaacg tgctcaagag
    12301 cctcggcatc ggcaccggtg accgggtctg catctacctg ccgatgatcc ccgaggccgt
    12361 cgtcgccatg ctggcctgcg cccgactcgg cgccgcgcac tcggtcgtct tcggcggctt
    12421 cagccccgac tcgctcatcg accgcatcga cgacgccggc gcgacggtcg tggtcacctc
    12481 cgatgccggg taccggcgcg gcaagccgtt ccttctcaag ccgaacgtcg acgtggccgt
    12541 cgccgcgtgc ccgacggtgc gctcggtcgt cgtcgtcgac cgctgccacg cggacgttcc
    12601 catggaggac ggccgggacc actggtggca caacctgatg gcggagcagt ccgaccactg
    12661 cccgccggtg tcggtcgacg ccgagcagct gctgttcctg ctctacacct cgggcaccac
    12721 cgcccgaccc aagggcatca tgcacacctc gggtggctac ctgacccagg tggcgtggac
    12781 ccacaaggtc accttcgacc tgcacgccga caccgacgtc tactggtgcg cggccgacat
    12841 cggctgggtg accggccaca gctacatcgt gtacggaccg ctcgccaacg gcgccaccac
    12901 cgtcatgtac gagggcaccc ccgacacgcc gcgggaggag ctgcgcaacg gcaccgaccc
    12961 cgccggctgg gacaaggacc ggctgtggga catcatcgag cgctacggcg tcacccagct
    13021 ctacaccgcg ccgaccgcga tccggacctt catgaagtgg ggcgccgagt ggcccgagcg
    13081 ccacgacctg tcgtcgctgc gggttctggg caccgtcggc gagccgatca accccgaggc
    13141 gtggatgtgg taccaccgcc acatcggctc ggagcgctgc ccgatcgtcg acacgtggtg
    13201 gcagaccgag accggcagcc agatggtcaa cccgctgccg ggcctgaccg ccaccaagcc
    13261 cggttcggcg tgccgcccgc tgccgggcat cggtgccgag ctggtcgacg acgacggcaa
    13321 cgtggtcacc cgcggcggcg gctacctgac cctcacacgg ccctggccgt cgatgctgcg
    13381 cggcatctgg ggcgacccgg agcgctaccg cgacacctac tggtcgcggt tcgagggccg
    13441 ctacttcgcc ggcgacggcg ccaagctcga cgaggacggc tacctgtggg tgctcggccg
    13501 ggtcgacgac gtcatgaacg tgtcgggcca ccgcatctcc accgccgagg tcgagtccgc
    13561 gctggtcgac cacccggcgg tggccgaggc ggcggtcgtc ggggccaccg acgccaccac
    13621 cggccaggcc atcgtcggct acgtcatcct gcggggcagc tacaccgcgt ccgaggagct
    13681 gcgcgaggag atccgtcagc acgtgtcgac caagctcggc gccatcgccc ggcccaaggc
    13741 cgtcatcctg gtgaccgatc tgcccaagac ccgctcgggc aagatcatgc ggcgactgct
    13801 gcgcgacgtg gtcgagggcc gagagctcgg cgacaccacc acgctggccg acgcgacggt
    13861 ggtcgaggcc atccgcgagg gcgccgcgac ctcgaccgag gactagctac cgcgcccgcc
    13921 gggcgggtcg ggcgagggcg tatcctgccc gccgtgccga cgtgggatcc ggacttcagc
    13981 aactggttgt ggcgcgacga cccgctcgat gccgccacct cgatcgtgtt cgacatggat
    14041 ggggtcctgt ccaacgccag cacccgccag cactacctcg agcacccccg tcgtgactgg
    14101 gagtcgttct tccaggcgtg cggcgacgac gagctcatcg acgaggtggc gcagctgctc
    14161 gacgtgctcg acaccgagct tcagatcgtg ctgctcacgg cccgaccgat ccgggtgcag
    14221 ccccagacgc tggcgtggct cgaacggtac gggctgcgct gggacgtgtt gatcatgcgg
    14281 gagtacggcg actacatggc tgcccggcac ttcaagcagc gcacggtcta cgagctgcgc
    14341 caccggggtc acgagctgca gctggccttc gaggacgacc cccgcaacgt ggcgatgttc
    14401 cacggcgagg gcgtcccgtg catctacatc cactccggct actacaactg agcgcttccc
    14461 gccctcgcct ccccgctttg tagtgcgaga tccaggcgaa cgcctggatc tcgcactaca
    14521 gaacggcggt tgggaggttg ggggtggggg gtcgacgacc tgctcgatca accccgctcg
    14581 atcaggactc ggtcgatgtc gccggagaag tagtcgcaga accgctcgac ctggtcacag
    14641 gccagcttcc cggcgatggt caacgggaac gtgttgtcga tcgagccgac cggcttgtca
    14701 cggcgcgcca cctcagctcc gtcgacgcgc agcaccaggt cgtcgccgtc gcgctcgcag
    14761 cgcacctcgt gccacctgcc gtcgacgatc gaccgccgcg accgggcgtg cgacgagccc
    14821 ttggagccga ggaacaggca ggcgacgcga ccgtcgtcca tgtcgatctt gaagtagccg
    14881 ccgggcacgc cgaactgacc cttttgcacc acgttcccct tcggcgaggt ggtgcgcaga
    14941 cggacggtca ccgcgtacgg cccgtcgccc gggttcagcc gcttgtcgtg cggaacctcg
    15001 gcgagtcgat cgactgccac cggcggctcg tcgggagcga ccgcggcgcg tcgctgtgcg
    15061 actgccccct gctcctcgac gcccgagctg acctgggagc cgatggtgcc atcgagcccg
    15121 tcgggtccgt cgtcgatcaa ggtttgagat cccggaggtt cgtccatcct ccagtccgcc
    15181 accacctcgg agatcgggct tccggacccg ctcttggtcg acgtcgagct cgagcgggac
    15241 ggagctgatg aggtggacgg atgtgccgtg tcccgtcccc ggttctgggc ggagtccgcg
    15301 accgcatcgt cgcctccgca ggcggaggac ccgagcgccg tgaccagggt gatcgccacg
    15361 accgcgccga tggagagggc ccggcggggc ggcacctcgc tcagaccgcc caggcgccgg
    15421 agcggagacg ggcgatgcgc tcggggaccg gggggtgcgt catgaacagg ttcgacgcgt
    15481 tgcgaccccg cagcgggttg acgatgtagg ccgacgcctc ggccgggttc accttcaccg
    15541 gcacccgctc ggcccccgcc tcgagcttct cgagcgcccg cgccaacggc tcgccggtgc
    15601 cgatcatgcg ggccgccgac gcgtcggcct cgtactcccg gctgcggctg atcgccatct
    15661 ggatcagagc ggcggcgatc ggggccagga tcaccatcag cagcgcgccg aacgggttgc
    15721 tgcgctcgtc ggagcggccc ccgccgaaga tcatggcgaa gcgggccatg ctggccacga
    15781 aggtgatgcc catcgccacc gccgcggcca ccgacccgat caggatgtcg cggttgcgca
    15841 cgtgggacag ctcatgggcc aggacgccgc ggatctcgtc ccagtcgagg atctgcagca
    15901 gcccctgggt gacgcacacc gcggcgtggt ccgggtcgcg cccggtggcg aaggcgttgg
    15961 gctgcgggtt gggcgacacg tacagcgtgg gcttggggat gccggcgcgc atggccagat
    16021 ccgccacgat ggcgtggtac tgcggcatct gctccgccgt gacctcgatg gccttggcgg
    16081 agcggatggc gagcttgtcc gacgcccagt aggagccgcc gcacatggcg aggccgatga
    16141 gcaggccgac gacgacaccg gtcggtcccc agtaggagcc gatgaacatg cacaacccgc
    16201 cgagcccggc cagcaacacg gcggtcttga ggttgttgcg cacatccgca gagtagggcg
    16261 gagccggggt gggggccgga cggcgctgaa cgggggctca gaggtccggc cgcgacgtca
    16321 gtcccggaag ttgccgaact gcagcgggat ctcgaagtcg tggtccttgc aggcggcgat
    16381 cacggcctgg aggtcgtcgc gcttcttggc ggtcacccgg acctgctcgc cctgggtctg
    16441 ggactggacg cccttgagcc cgagcgcctt gatgaacttg ttgaggtcct tggcccggtc
    16501 cgagttgatg ccggcgacga gcttggcgac ctgacgcacg gtggcccccg acgcctcctc
    16561 gaccttctcg taggacagcg ccttcatcga gatgccccgc ttgacgaact tctcctccag
    16621 cacctgccgc agcgcggcca gccggtcctc ggtcgaggag cgcagcgtga tggcgtcgtc
    16681 gccgaggtcg agcgacgagc cggtgtcctt gaagtcgaac cggttcgcga cctcgcggga
    16741 cgcctggtcg accgcgttcc ggatctcctg ggcgtcgacc tcggaggtga tgtcgaagct
    16801 gggcatctgt gaccttcctg actggtgcgg ccgatcgccg ggtgcggacc cgccgtcgga
    16861 tcgggttcgt tcgtcgttga cgcgaacgct atcgtgaccg cttctgcggg tcggtgcccg
    16921 agcggccaaa gggagcagac tgtaaatctg ccggcgtatg ccttcggagg ttcaaatcct
    16981 ccccggccca ccaacgcgac gatgacggca ggccccggtg gcctgccgtc tgcgcgtccg
    17041 ggccgcgctc agagcagtcg ctccatgacg accacgtcgt ggaagcgtcc gaacttgcgg
    17101 cccacctcct tctcccggcc gacgatctcg aagccgaggc gggcgtgcag gccgatcgag
    17161 gcgtcgtggc cggcgacgat gcgggcgaac acggcgtgga agccgtgcgc ggtcgcccgt
    17221 tcgaccagtt cgctcagcag cgccacgccc acgccgcgac cccggtgggc atcgtggacg
    17281 tagaccgagt cctcgaccga cgtcgagtag cccggccggt cccggtacgg cgacagggct
    17341 ccgaacccga tgacgacctc ggagccgtcg tcggcatcca cggccaccac cgcggcgtgg
    17401 gcgccggagc ggtcggtgat ccaccggcgt tggtcctcga gggtgcgggg ggtcaggtcg
    17461 aacgtgacgt tcgaggtgag gacctcgtgg ttgtagatct cgaggatggc cggggcgtcg
    17521 gccgcgaccg cggggcgcag gatcacccgg gcgacgctac cggtcccgac gcggacccca
    17581 gcgcagggtt tctgtggtcg ggattgtgac aagggttgtg agtgcggacc cctatatcga
    17641 gggttggacg acgatggcgc tgggttcgat gacgccgatg gcgccggtca tcgcctcgac
    17701 ccgggcccac tccgacaccg tcgccgactc gctcagcagg atgatctgct ggtgctccga
    17761 gctgcgcacg agcagctcca acatggccgg cagggt
//
LOCUS       DAOQOD010000051        17287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1165_length_17287_cov_13.157149, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000051 DAOQOD010000000
VERSION     DAOQOD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17287
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_17287_cov_13.157149"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..649)
                     /gene="rpsD"
                     /locus_tag="PLZ72_06875"
     CDS             complement(32..649)
                     /gene="rpsD"
                     /locus_tag="PLZ72_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNA36056.1"
                     /translation="MSRYTGPRARVSRRLGTNIWGTNGENIAMEKRPYPPGEHGRTRR
                     RGNVSEYLLQLQEKQKARFAYGMTEKQFRNLYEEANRRQGVTGENLLRYCEQRLDNVV
                     YRATWAATRPQARQFVTHGHVEVNGRRVNVPSYRVRKGDVIRLREKSRQMLVIQWNMD
                     TLGRVAAPWLDTTDGGQEVTVRELPLREHIDVPVREQLIVELYSK"
     gene            complement(653..1051)
                     /gene="rpsK"
                     /locus_tag="PLZ72_06880"
     CDS             complement(653..1051)
                     /gene="rpsK"
                     /locus_tag="PLZ72_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNA36057.1"
                     /translation="MAKPPAGGRRPRKKERKHVTHGVAHIKSSFNNTIISITDLEGNV
                     LAWASAGNAGFKGSRKSTPFAAQLAAEQAARRAMEHGVRKVDIVVKGPGSGRETAIRS
                     IQNVGIEVTGIKDVTPVPHNGCRPPKRRRL"
     gene            complement(1051..1428)
                     /gene="rpsM"
                     /locus_tag="PLZ72_06885"
     CDS             complement(1051..1428)
                     /gene="rpsM"
                     /locus_tag="PLZ72_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNA36058.1"
                     /translation="MARIAGVDIPREKRLVISLTYIYGIGRTASEQICDTAGIDPSTR
                     VRDLTDAEVTQLRSVIEEGYKVEGDLRREVNQNIKRKMEIGCYQGLRHRRGLPVRGQR
                     THTNARTRKGPKKTVAGKKKVRK"
     gene            complement(1463..1576)
                     /gene="rpmJ"
                     /locus_tag="PLZ72_06890"
     CDS             complement(1463..1576)
                     /gene="rpmJ"
                     /locus_tag="PLZ72_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNA36059.1"
                     /translation="MKVKPSVKKICEKCRVIRRNGRVMVICDNPRHKQRQG"
     gene            complement(1643..1867)
                     /gene="infA"
                     /locus_tag="PLZ72_06895"
     CDS             complement(1643..1867)
                     /gene="infA"
                     /locus_tag="PLZ72_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNA36060.1"
                     /translation="MPKPKEDAIVLEGTVIEPLPNAMFRVELENGHKVLAHISGKMRM
                     HYIRILPGDKVQVELTPYDLTRGRITYRYK"
     gene            complement(2035..2712)
                     /locus_tag="PLZ72_06900"
     CDS             complement(2035..2712)
                     /locus_tag="PLZ72_06900"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNA36061.1"
                     /translation="MSSLRLIVFGRQGAGKGTQAVRLADHYGIPHISTGDMLRAAAAS
                     GSDFGRQVKEIMDSGSLVSDEVMEGVVAERLAQPDAAAGFLLDGYPRTPGQAEYLQTL
                     LAPDGVQAAINLEVPEDVVVDRITGRRVCSSCGATYALGRDESAVSGLCANCGGEVLQ
                     RPDDTEDAVRKRLALYAESTEPLLNWFRERDLLVDVDGVGDPDDIAAALVARIDERRF
                     GAPGAGS"
     gene            complement(2802..4133)
                     /gene="secY"
                     /locus_tag="PLZ72_06905"
     CDS             complement(2802..4133)
                     /gene="secY"
                     /locus_tag="PLZ72_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNA36062.1"
                     /translation="MSRIGNIIKVPDLRNKVLFTLMMLVLYRFGSYVPVPGIDQNAVA
                     AIEDQARQGGVLAFLQLFSGRALTRFSLFALGIMPYITASIIMQILAVVIPKLQEWQQ
                     QGAVGQRKTTQWTRYVTIGISILQSTGLVFLFHNGGGGLTGGQSNLDLVPGFSPAIVM
                     LIVLTFTAGSALLMWMGELITTRGIGNGMSLIIFCSVVSGLPSAFSAVYAAENGAVKL
                     AIFIAFFLVLLVFIVFIELGQRRIPVQFAKRVVGRRMYSGQSTYIPLKVNQAGVIPII
                     FASAVINLPVLLSQVLPSDGWGASVSKFINDYLADTTNMVYLAFLGLLIVGFTYFYTA
                     IAFDPVQQADNLRKQGGFIPGIRPGPQTEHYLTKVLNRITLPGALFVAILAISPTILV
                     GVLLGSSASGAAYSIGGSSLLIAVGVALETMKQVDSQLMMRNYEGFLSAKK"
     gene            complement(4293..4778)
                     /gene="rplO"
                     /locus_tag="PLZ72_06910"
     CDS             complement(4293..4778)
                     /gene="rplO"
                     /locus_tag="PLZ72_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNA36063.1"
                     /translation="MKIHDLKPADGSRKRRKRVGRGIGGKGGKTAGRGTKGQKARDTI
                     PVSFEGGQLPLSMRVPKLRGFNNPFRVEYQAINLDTIAASGLDEITPDTLRSKGLVSK
                     GALVKVLGRGEIDRKVTVKVHAVSASAEAAITAAGGTVEKLPLPFAVRPAFSGSAHTN
                     R"
     gene            complement(4780..4962)
                     /gene="rpmD"
                     /locus_tag="PLZ72_06915"
     CDS             complement(4780..4962)
                     /gene="rpmD"
                     /locus_tag="PLZ72_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNA36064.1"
                     /translation="MAQIKVTQVRSSIGTKPKQRGTLRALGLGRIGKSHVLEDTPDLR
                     GQLQKVPHLIEIEELD"
     gene            complement(4962..5525)
                     /gene="rpsE"
                     /locus_tag="PLZ72_06920"
     CDS             complement(4962..5525)
                     /gene="rpsE"
                     /locus_tag="PLZ72_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNA36065.1"
                     /translation="MAPNNDMDLRESRVININRVAKVVKGGRRFSFTALVVIGDGNGR
                     VGLGYGKAKEVPLAIQKGTEEARKNLFTVPLAGGTIIHPVIGVVGAGRVLLKPAAPGT
                     GVIAGGAARAILEEAGIHDVLCKSLGSSNYINIAKATVDGLRQQRRPDEIAALRGLPV
                     DEIIPAGLLRAYQESERGPAPEPTEVA"
     gene            complement(5525..5890)
                     /gene="rplR"
                     /locus_tag="PLZ72_06925"
     CDS             complement(5525..5890)
                     /gene="rplR"
                     /locus_tag="PLZ72_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNA36066.1"
                     /translation="MSDKSKQKRDSRIRRHARVRRQVMGTPERPRLAVFRSNRHVVAQ
                     VIDDRAGVTLAAASTLEGDLRSGATGNVAAAARVGTLVAERAKAAGIEQVVFDRGGFR
                     YHGRVAALADAARDAGLEF"
     gene            complement(5904..6440)
                     /gene="rplF"
                     /locus_tag="PLZ72_06930"
     CDS             complement(5904..6440)
                     /gene="rplF"
                     /locus_tag="PLZ72_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNA36067.1"
                     /translation="MSRIGKAPIAVPSGVTVTLDGQSISVKGPKGELSRTYSELISVR
                     QDGEELVVERSGDDRDSRAQHGLVRSLIQNMVTGVTQGFQKDLEIVGVGYRAQAKGQG
                     LELSLGFSHPVVVDAPDGISFNVPAPTRIEVHGIDKQLVGQVAANIREWRKPEPYKGK
                     GVRYADEHVRRKAGKAGK"
     gene            complement(6450..6854)
                     /gene="rpsH"
                     /locus_tag="PLZ72_06935"
     CDS             complement(6450..6854)
                     /gene="rpsH"
                     /locus_tag="PLZ72_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNA36068.1"
                     /translation="MTMTDPIADMLTRIRNANIAMHDDVRMPSSKLKEALAGLLQKEG
                     YIVGFSVTDNPSGPGQFLNIDMKYSEERRRVISGIRRVSKPGLRVYRKHDELERVLGG
                     LGIAVLSTSKGLMTDREARRQRVGGEVLCHVW"
     gene            complement(6871..7056)
                     /locus_tag="PLZ72_06940"
     CDS             complement(6871..7056)
                     /locus_tag="PLZ72_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNA36069.1"
                     /translation="MATEALKQKQQRKPKFKVRAYTRCRRCGRPRSVYRKFGLCRVCL
                     RELAHAGEIPGVTKASW"
     gene            complement(7056..7598)
                     /gene="rplE"
                     /locus_tag="PLZ72_06945"
     CDS             complement(7056..7598)
                     /gene="rplE"
                     /locus_tag="PLZ72_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNA36070.1"
                     /translation="MKTRYNDEIRSQLLESLGLGNIMEVPRLEKIVVNVGAGKATENR
                     GLLDKVVEDLTAICGQKPVITKAKKSIAGFKLREGNAIGVKVTLRGDNMWEFFDRLIT
                     LAIPRIRDFRGLSSKSFDGRGNYTFGVTEQLIFPEIDYDKIDASRGMDITIVTTARTD
                     AEGRALLDAFGFPFRREGQQ"
     gene            complement(7625..7930)
                     /gene="rplX"
                     /locus_tag="PLZ72_06950"
     CDS             complement(7625..7930)
                     /gene="rplX"
                     /locus_tag="PLZ72_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNA36071.1"
                     /translation="MKIRKGDRVRVLSGKDKGKEGEVMSVDPAANKAIVEGVNVAKRH
                     QKPTRSMQQGGIIDKAMPIHVSNLALIDSTGKATRAGYRIGADGTKTRVARSNGGEL"
     gene            complement(7943..8311)
                     /gene="rplN"
                     /locus_tag="PLZ72_06955"
     CDS             complement(7943..8311)
                     /gene="rplN"
                     /locus_tag="PLZ72_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNA36072.1"
                     /translation="MIQQESRLRVADNSGAREVLVIKVLGGSRRRYASIGDIFVATVK
                     DALPGAAVKKGDVVKCVVVRTKKERRRPDGSYIRFDENAAVLINDQKLPRGTRIFGPV
                     GRELRDKKFMRIVSLAPEVI"
     gene            complement(8308..8589)
                     /gene="rpsQ"
                     /locus_tag="PLZ72_06960"
     CDS             complement(8308..8589)
                     /gene="rpsQ"
                     /locus_tag="PLZ72_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNA36073.1"
                     /translation="MSDDTATTGTERNARKVREGIVTSNKMERTAIVTVTERVRHPRY
                     NKTMQQDKKLYVHDETNDLNVGDRVRVMETRPLSKLKRWRVLEIVERAR"
     gene            complement(8586..8813)
                     /gene="rpmC"
                     /locus_tag="PLZ72_06965"
     CDS             complement(8586..8813)
                     /gene="rpmC"
                     /locus_tag="PLZ72_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNA36074.1"
                     /translation="MAKDTSVASLGDEQLIERLAEEKDRLFKLRFQLATGQLENSARI
                     GQTKKDIARINTELRAREIAAAEAAIGSEAS"
     gene            complement(8813..9229)
                     /gene="rplP"
                     /locus_tag="PLZ72_06970"
     CDS             complement(8813..9229)
                     /gene="rplP"
                     /locus_tag="PLZ72_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNA36075.1"
                     /translation="MLMPRKVKHRKQHRGRTKGVSKGGTNVTHGQYGIQALEPGWITA
                     RQIEAARIAMTRHIKRGGKVWINVFPDKPVTQKPAETRMGSGKGNPERWVAVVRPGRV
                     LFELSYPDDAIARGALNRAIQKLPVKAKIITREEEI"
     gene            complement(9229..10149)
                     /gene="rpsC"
                     /locus_tag="PLZ72_06975"
     CDS             complement(9229..10149)
                     /gene="rpsC"
                     /locus_tag="PLZ72_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNA36076.1"
                     /translation="MGQKVNPYGFRLGTTTDWKSRWFATREEYVDYLIEDWKIRDYLM
                     NKLPNAAISRIEVERTRDRLRVDVHTARPGIVIGRRGAQADELRADLTKLTGNPKVQL
                     NIQEIKQPEVDAALIAQGVADQLANRIAFRRAMKRAVQNAQKAGALGIRVQCSGRLGG
                     SEMSRTEWYREGRVPLHTLRADIDYGFREARTPAGRIGVKVWIYKGDILPYKSANEDK
                     ITKEAAMAVGETAGQERPRVVSTKPVAPAAAAAPASPAPAPEVSDEDLKPLVQEADPE
                     FERLLAEEEEIERSTREHHETPHFRPGDAD"
     gene            complement(10509..10856)
                     /gene="rplV"
                     /locus_tag="PLZ72_06980"
                     /pseudo
     CDS             complement(10509..10856)
                     /gene="rplV"
                     /locus_tag="PLZ72_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(10853..11137)
                     /gene="rpsS"
                     /locus_tag="PLZ72_06985"
     CDS             complement(10853..11137)
                     /gene="rpsS"
                     /locus_tag="PLZ72_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNA36077.1"
                     /translation="MPRSLKKGPFVDDHLLKKVDDLNEKGQKNVIKTWSRRSTIIPEM
                     VGHTIAVHDGRKHVPVYVTESMVGHKLGEFAPTRTFKFHAGQEKGARGRR"
     gene            complement(11153..11989)
                     /gene="rplB"
                     /locus_tag="PLZ72_06990"
     CDS             complement(11153..11989)
                     /gene="rplB"
                     /locus_tag="PLZ72_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNA36078.1"
                     /translation="MAVRKRKPTSPGRRFQTVSDFSDITRSTPEKSLVAKKSKTGGRN
                     NHGRKTARHRGGGHKQQYRVIDFKRRKDGVPAKVAAIEYDPNRTCRIALLHYLDGEKR
                     YILAPKGLNVGDMVQSGQGAEIRSGNALPLRYIPVGTVVHNVEFKPGAGGKMARSAGS
                     SVQLVAKEGDYATLRLPSTEMRRVPIDCRATVGEVGNSEHDLVKIGKAGRNRWKGVRP
                     QTRGVAMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRNKNKPSQKLLVRRRRTRGS
                     RR"
     gene            complement(11991..12287)
                     /gene="rplW"
                     /locus_tag="PLZ72_06995"
     CDS             complement(11991..12287)
                     /gene="rplW"
                     /locus_tag="PLZ72_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNA36079.1"
                     /translation="MKDPRDVIIRPVVSEKSYGLLDTGVYTFIVAPSASKPEIRDAVQ
                     AIWPGVKVKKVNTLNRKGKKRRNRRSPTFGSRPDTKRAVVTLAEGDQIDLFEVK"
     gene            complement(12290..12946)
                     /gene="rplD"
                     /locus_tag="PLZ72_07000"
     CDS             complement(12290..12946)
                     /gene="rplD"
                     /locus_tag="PLZ72_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNA36080.1"
                     /translation="MASITLHDASGASTGTVELDDATFGIEPNVAVMHQVVTAQLAAK
                     RAGTQSTKTRAEVAGGGAKPWKQKGTGRARAGSSRSPIWRGGGVALGPKPRKYDQKTP
                     KKMKRLALRSALSDRAADDKVVVVSAWDFDTPSTKAAAAALAAMGVEGRALVVLSPDD
                     VTAWKSFRNLPSVHVLTAGELNTYDVLVSDHVVFTEATLPTSAPAVAEPAADTSEEGA
                     "
     gene            complement(12946..13608)
                     /gene="rplC"
                     /locus_tag="PLZ72_07005"
     CDS             complement(12946..13608)
                     /gene="rplC"
                     /locus_tag="PLZ72_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNA36081.1"
                     /translation="MAKTAALVGEKLGMTQVWDESNRIVPVTVLRVSPARVVQIRTPE
                     KDGYSALQVTYGHRDARKLTKPLAGHYAKGDVDPGVRLVELRLDDISAYEVGQTLDAD
                     TFAAGDLIDVTAVSKGKGFAGAMKRHGFKGQGASHGAHRVHRKPGSIGQCATPARVFK
                     GVRMAGRTGGTKVTTLNLEVVKADTDQEVILVRGAVPGPTGGVVLLRNAVKRSASKEG
                     AA"
     gene            14079..15131
                     /locus_tag="PLZ72_07010"
     CDS             14079..15131
                     /locus_tag="PLZ72_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36082.1"
                     /translation="MTSTDPAVVADGLVKRFGDTEALRGVSFEIPRATVLGVLGPNGA
                     GKTTAVRILSTLLRADGGRALIDGIDVGRHPRQARARIGLTGQYAAVDERLTGIENLQ
                     LVGRLFHMRKADAAARGRELLDRFSLDQAADRVVKGYSGGMRRRLDIAMSLIARPSVL
                     FLDEPTTGLDPRSRLEMWDVIDGLVADGTTLLLTTQYLDEAERLADDIVVIDKGTVIA
                     RGTEEELKARSGGSSVTVTLSDPADLPRVRGLLADLVPAGAVESVDAVARTVSVPVTR
                     TDHVVPTVVRALDAAGLDVLDVAAHEPTLDDVFLQLTGHTATTDDPGVDADSGPEPGR
                     PATSADTPPTTAGAPR"
     gene            15128..15964
                     /locus_tag="PLZ72_07015"
     CDS             15128..15964
                     /locus_tag="PLZ72_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA36083.1"
                     /translation="MTTTAPDPSVAAPSTISSHTVPTGPRWLASDIRVEAGRHLTATW
                     RNPDLLVFATIQPIMFVVLFVYVFGGAITVPGYSSYQQFVVPGIFAQTVVFGSAFTGI
                     GIAEDMQKGFIDRLRSLPMYPSAVLVGRTTSDLVRNTFTFAIMMIVGLIVGFRLEGGL
                     ANAVLATVLLLAFAFAFSWIQALIGLSVKSVEAANSAGFIWMFPMTFVSSAFVDPSTM
                     PGWLQPIAEHNPFTVVTNAVRALYNGMDPGNDLWISIAWAVGITVVFGALSFRKFSSA
                     SR"
     gene            complement(16162..16482)
                     /gene="rpsJ"
                     /locus_tag="PLZ72_07020"
     CDS             complement(16162..16482)
                     /gene="rpsJ"
                     /locus_tag="PLZ72_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNA36084.1"
                     /translation="MAADQKIRIRLKAYDHEVIDQSTKKIVETVTRTQASVRGPIPLP
                     TEKHRFTVIRSPHKDKDSREHFEMRIHKRLIDIIEPTPKTVDSLQRLDLPAGVDIEIK
                     IQNA"
     gene            16633..17097
                     /locus_tag="PLZ72_07025"
     CDS             16633..17097
                     /locus_tag="PLZ72_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA36085.1"
                     /translation="MSEPIAAVIDTWHEFLRGARPDALDELLDDDVVFHSPIVFTPQR
                     GKAITTLYLTAATQALPGDAPTGDDAGDHPADVAMPGGSFRYTKQVLSGDTAVLEFET
                     TIDGLYVNGVDIIRCNPDGRIVEFRVMIRPLQAVNLVHRQMAAMLESMSSSG"
BASE COUNT         2359 a         5781 c         6157 g         2990 t
ORIGIN      
        1 tgaagccttc ctcaggggtt tcggcgggcg gttacttgga gtagagctcg acgatgagct
       61 gttcgcgcac cgggacgtcg atgtgctcgc gcagcgggag ctcgcggacg gtgacctcct
      121 ggccgccgtc ggtcgtgtcg agccaggggg cggcgacgcg accgagggtg tccatgttcc
      181 actggatgac cagcatctgg cgggacttct cgcgaaggcg gatgacgtcg cccttgcgga
      241 cccggtagct gggcacgttg acccggcggc cgttgacctc gacgtggccg tgggtcacga
      301 actggcgggc ctgcgggcgg gtggccgccc acgtggcgcg gtacacgacg ttgtcgaggc
      361 gctgctcgca gtagcgcagc aggttctcgc cggtgacgcc ctggcggcgg ttcgcctcct
      421 cgtagaggtt gcggaactgc ttctcggtca tgccgtaggc gaagcgggcc ttctgcttct
      481 cctgcagctg gagcaggtac tcgctgacgt tgccccgacg gcgggtgcgg ccgtgctcgc
      541 cgggcgggta ggggcgcttc tccatggcga tgttctcgcc gttggtgccc cagatgttgg
      601 tgccgagccg gcgcgagacg cgggcgcggg ggccggtgta gcgggacatg ggtcagagcc
      661 tccgacgctt ggggggacgg cagccgttgt gcggcacggg ggtgacgtcc ttgatgccgg
      721 tcacctcgat gcccacgttc tggatggatc ggatggcggt ctcgcggccc gagcccgggc
      781 ccttgaccac gatgtcgacc ttgcgcacgc cgtgctccat ggcccgccgg gcggcctgct
      841 cggccgcgag ctgggcggcg aacggggtgg acttgcggga gcccttgaac ccggcgttgc
      901 ccgccgaggc ccacgccagg acgttgccct cgaggtcggt gatcgagatg atcgtgttgt
      961 tgaacgagct cttgatgtga gcgacgccgt gggtgacgtg cttgcgttcc ttcttccggg
     1021 gacgacggcc cccggccggt ggctttgcca ttacttgcgg accttcttct tgccggcgac
     1081 ggtcttcttg ggacccttgc gggtgcgggc gttggtgtgg gtgcgctgac cccggaccgg
     1141 caggccgcga cggtggcgca ggccctggta gcagccgatc tccatcttgc gcttgatgtt
     1201 ctggttgacc tcacgacgga ggtcgccctc gaccttgtag ccctcttcga tcaccgatcg
     1261 gagctgggtc acctcggcgt cggtcaggtc ccggacgcgg gtggacgggt cgatcccggc
     1321 ggtgtcgcag atctgctcgg acgcggtgcg accgatgccg tagatgtagg tgagggagat
     1381 gacgaggcgc ttctccctcg ggatgtcgac gccggcgatg cgtgccattg gttcctgtcc
     1441 tgctttctcg gaggcgagct gctcagccct ggcgctgctt gtggcgcggg ttgtcgcaga
     1501 tgaccatcac ccgaccgttg cggcggatga cacggcactt ctcgcagatc ttcttgacgc
     1561 tgggcttgac cttcatgcgg acttctcgat cattcgacgg ccgtcggggg tgacggcgct
     1621 cggttgcggt gacgaccgtg cgtcacttgt agcggtaggt gatccgaccc cgggtgaggt
     1681 cgtagggggt gagctccacc tggaccttgt cccccggcag gatccggatg tagtgcatcc
     1741 gcatcttccc ggagatgtgc gccaggacct tgtgcccgtt ctcgagctcg acccggaaca
     1801 tggcgttggg gagcggctcg atgaccgtcc cctcgaggac gatggcgtct tccttcggct
     1861 tgggcagaac acttcctcct gatggatccc cagtcgcacg gactacagcg gggtgggtcg
     1921 cacggcgcgt gcaaggacgg ggtcctgacg ggccgacggg caaatatatg tcaacggtcc
     1981 gccgcgatca aaggcggctg tgcgaggctc cccggtcggg tcgatcccgg ccggtcacga
     2041 ccccgccccg ggggcaccga aacggcgttc gtcgatccgg gccaccagcg ccgcggcgat
     2101 gtcgtcgggg tcgccgacgc cgtcgacgtc gacgagcagg tcccgctccc ggaaccagtt
     2161 gagcagcggt tcggtcgact cggcgtacag ggccagccgt ttgcggacgg cgtcctcggt
     2221 gtcgtcggga cgctgcagca cctcaccccc gcagttggcg cacagcccgg agaccgccga
     2281 ctcgtcgcgg ccgagggcgt aggtggcgcc gcacgacgag cagacccgtc gaccggtgat
     2341 gcggtcgacg acgacgtcct cgggcacctc gaggttgatc gccgcctgca cgccgtcggg
     2401 ggcgagcagc gtctggaggt actcggcctg accgggggtg cgcgggtagc cgtcgagcag
     2461 gaaccccgcc gccgcgtcgg gctgcgcgag ccgctcggcc accacgccct ccatgacctc
     2521 gtcgctgacc agcgaaccgg agtccatgat ctccttgacc tgacggccga agtccgatcc
     2581 cgacgccgcg gcggcccgca gcatgtcgcc ggtcgagatg tgggggatgc cgtagtggtc
     2641 ggccaggcgc accgcctgag tgcccttccc cgcgccctgc cggccgaaga cgatcagtcg
     2701 cagtgagctc atggcgtact ccgtggggtg acacccgggc ccgctcggcg aaccggggcg
     2761 ggcggagggg acggacggga cggcggcaga gtcgccaccg gtcacttctt ggccgacagg
     2821 aatccttcgt agttgcgcat catcaactgg ctgtcgacct gcttcatggt ctcgagcgcc
     2881 acgccgacag cgatcagcag cgagctgccg ccgatcgagt aggcggcgcc gctggcgctc
     2941 gaacccagca ggaccccgac gaggatcgtc ggcgagatgg ccaggatggc cacgaacagc
     3001 gcgccgggca gggtgatgcg gttgagcacc ttggtcaggt agtgctcggt ctgcggtccg
     3061 ggtcggatgc cggggatgaa cccgccctgc ttgcgcaggt tgtcggcctg ctggaccggg
     3121 tcgaaggcga tcgccgtgta gaagtaggtg aagccgacga tgagcaggcc caggaacgcc
     3181 aggtacacca tgttggtcgt gtcggccagg tagtcgttga tgaacttcga caccgacgcc
     3241 ccccagccgt cgctgggcag gacctggctg agcaggaccg gcaggttgat caccgccgag
     3301 gcgaagatga tcgggatcac gccggcctgg ttgaccttga gcgggatgta ggtgctctgc
     3361 cccgagtaca tgcgccgacc gaccacccgc ttggcgaact gcaccgggat gcggcgctgg
     3421 ccgagctcga tgaagacgat gaacacgagc agcaccagga agaaggcgat gaagatcgcc
     3481 agcttcaccg ctccgttctc ggccgcgtac accgccgaga acgcggaggg cagacccgac
     3541 acgaccgagc agaagatgat cagcgacatg ccgttgccga tgccgcgggt ggtgatcagc
     3601 tcgcccatcc acatcagcag cgccgagccc gcggtaaagg tcagcacgat cagcatcacg
     3661 atcgcgggcg agaagcccgg cacgaggtcc aggttggact gaccgccggt gaggccaccg
     3721 ccgccgttgt ggaacaggaa caccaggccg gtcgactgca ggatcgagat gccgatggtg
     3781 acgtagcggg tccactgggt ggtcttgcgc tgaccgaccg ccccctgctg ctgccactcc
     3841 tgcagcttgg ggatcaccac ggccaggatc tgcatgatga tcgacgccgt gatgtacggc
     3901 atgatcccca gcgcgaacag gctgaagcgg gtcagggccc gaccggagaa cagctggagg
     3961 aacgccagca cgccaccctg gcgggcctgg tcctcgatgg cggccacggc gttctggtcg
     4021 atgcccggca cgggcacgta gctgccgaag cggtacagga cgagcatcat cagggtgaac
     4081 aggaccttgt tgcggaggtc cggcaccttg atgatgttgc cgattcggga cactgcagct
     4141 cctgggcgga ttggtgtcgg gcggacccga tcaccgtcga ccggttcggt tcgggcctga
     4201 gcggatgcga tcggcgcctg cggccggtgg ccgagggtag ccgagcgccg ccgccccgac
     4261 ggggtccggg gccgctcggg gccccggatc cgtcagcggt tggtgtgggc ggagccggag
     4321 aaggccggcc gcacagcgaa cggcagcggc agcttctcca ccgtgccccc cgcggcggtg
     4381 atcgccgcct cggccgaggc cgacacggcg tgcaccttca cggtgacctt gcggtcgatc
     4441 tcgccgcgac cgagcacctt gaccagcgcc cccttggaca cgaggccctt ggagcgcagc
     4501 gtgtcggggg tgatctcgtc gaggcccgac gccgcgatcg tgtcgaggtt gatcgcctgg
     4561 tactcgaccc ggaacgggtt gttgaagccg cgcagcttgg gcacccgcat ggacaacggg
     4621 agctggccgc cctcgaagct caccgggatg gtgtcgcgcg ccttctggcc cttggtgccg
     4681 cggccggcgg tcttgccgcc cttgccgccg atgccgcgac cgacgcgctt gcgccgcttg
     4741 cgcgacccgt cggccggctt gaggtcatgg atcttcatgt cagtccagct cctcgatctc
     4801 gatgaggtgc ggcaccttct ggagctggcc ccgcaggtcg ggggtgtcct ccaggacgtg
     4861 gctcttgccg atccggccga ggccgagggc tcgcagggtg ccgcgctgct tgggcttggt
     4921 gccgatcgag gaccgcacct gggtgacctt gatctgagcc atcacgccac ctcggtgggc
     4981 tcgggcgccg gaccccgctc ggactcctgg taggcccgaa gcaggcccgc cgggatgatc
     5041 tcgtcgaccg ggagaccccg cagcgcggcg atctcgtcgg gccggcgctg ctgacggagg
     5101 ccgtcgacgg tggccttggc gatgttgatg tagttcgacg agccgagcga cttgcacagc
     5161 acgtcgtgga tgccggcctc ttcgaggatg gcgcgggccg caccgccggc gatgacgccg
     5221 gtaccgggag cggcgggctt gagcaggacg cgcccggcgc cgaccacgcc gatgaccggg
     5281 tggatgatgg tgccgccggc gagggggacg gtgaagaggt tcttgcgcgc ctcctcggtc
     5341 cccttctgga tggcgagcgg cacctccttg gcctttccgt agccgaggcc gacccggccg
     5401 ttgccgtcgc cgatcaccac cagggcggtg aaggagaacc gacggccgcc cttcaccacc
     5461 ttggcgacgc ggttgatgtt gatgacccgt gactcgcgca ggtccatgtc gttgttcggg
     5521 gccatcagaa ctccagtcct gcgtctcggg cggcatccgc gagggcggcg acgcgaccgt
     5581 ggtagcggaa gccgccccgg tcgaagacca cctgctcgat gccggcggcc ttggcccgct
     5641 cggcgacgag cgtgccgacc cgggccgcgg cggcgacgtt gccggtggca ccggagcgca
     5701 ggtcgccctc cagcgtggag gcggcggcga gggtgacacc ggcgcggtcg tcgatcacct
     5761 gggcgaccac gtggcggttc gatcggaaca cggccaggcg cggacgctcg ggggtgccca
     5821 tgacctggcg gcgcacccgg gcgtgacgac ggatgcggct gtcacgcttc tgcttgctct
     5881 tgtcactcat ggttgcgcac ctgtcacttg ccggccttgc cggccttgcg gcggacatgc
     5941 tcgtcggcgt agcgcacgcc cttgcccttg tagggctccg gcttgcgcca ctcacggatg
     6001 ttggcggcca cctggccgac cagctgcttg tcgatcccgt ggacctcgat gcgggtcggc
     6061 gccgggacgt tgaaggagat gccgtcgggg gcgtcgacca cgaccgggtg cgagaacccg
     6121 agcgacagct cgaggccctg ccccttggcc tgggcccggt agccgacgcc gacgatctcc
     6181 aggtccttct ggaagccctg ggtcacgccg gtcaccatgt tctggatcaa ggagcggacc
     6241 aggccgtgct gggcccggga gtcgcggtcg tcgccgctgc gctccaccac gagctcctcg
     6301 ccgtcctggc ggaccgagat cagctcggag taggtgcgcg acagctcgcc cttggggccc
     6361 ttgaccgaga tggactgccc gtcgagggtg acggtcacgc cgctcgggac ggcgatgggt
     6421 gccttgccga ttcgagacat gggctcacct caccagacgt ggcagaggac ctcaccaccg
     6481 acccgctggc ggcgcgcctc gcggtcggtc atgagtccct tgctcgtgga caggacggcg
     6541 atgccgaggc ctccgaggac gcgctcgagc tcgtcgtgct tgcggtagac gcgaagtccc
     6601 ggcttggaga cacgacggat gccggagatc acccggcgtc gctcctcgga gtacttcatg
     6661 tcgatgttca ggaactggcc ggggcccgac gggttgtcgg tcacggagaa cccgacgatg
     6721 tagccctctt tctggagcag gccggcgagg gcctccttca gcttggacga gggcatcctg
     6781 acgtcgtcgt gcatcgcgat gttcgcgttg cggatgcggg tcagcatgtc ggcgatgggg
     6841 tcggtcatgg tcatgtcgtg ctccctcccc tcaccagctg gccttggtca cgcccgggat
     6901 ctctcccgcg tgtgcgagct ctcgcaggca cacgcggcag aggccgaact tgcggtacac
     6961 cgagcgcggc cggccacacc ggcggcaccg ggtgtaggcc cgcaccttga acttgggctt
     7021 gcgctgctgc ttctgcttca gagcctcggt cgccattact gctgcccttc acgacggaac
     7081 gggaagccga aggcgtccaa cagggcgcgg ccttcggcat cggttcgagc ggtggtcacg
     7141 atcgtgatgt ccatgccccg ggaggcatcg atcttgtcgt agtcgatctc ggggaagatc
     7201 aactgttcgg tcacgccgaa ggtgtagttg ccccggccgt cgaagctctt cgacgacagg
     7261 ccccggaagt cgcggatgcg ggggatcgcc agcgtgatca gccggtcgaa gaactcccac
     7321 atgttgtcgc cgcgcagcgt caccttgacg ccgatggcgt tgccctcgcg gagcttgaag
     7381 cccgcgatcg acttcttggc cttggtgatg accggcttct gaccgcagat ggcggtcagg
     7441 tcctcgacga ccttgtcgag caggccgcgg ttctcggtcg ccttgccggc gccgacgttg
     7501 accacgatct tctcgaggcg ggggacctcc atgatgttgc ccaggcccag ggactccagc
     7561 aactgcgagc ggatctcgtc gttgtaacgg gtcttcaggc gggggacgat cgccgtggcg
     7621 gtgctcacag ctcacctccg ttgctgcggg cgacacgggt cttggtcccg tccgctccga
     7681 tgcggtagcc ggctcgggtc gccttgccgg tgctgtcgat cagggccagg ttcgacacgt
     7741 ggatcggcat cgccttgtcg atgatgccgc cctgctgcat ggagcgggtg ggcttctggt
     7801 ggcgcttggc cacgttgacg ccctcgacga tcgccttgtt ggcggccggg tcgaccgaca
     7861 tgacctcgcc ctccttgccc ttgtccttgc cggacaggac ccggacgcgg tcgcccttgc
     7921 ggatcttcag gttcgccatg ggtcagatca cctccggggc gagcgacacg atgcgcatga
     7981 acttcttgtc gcgcagctca cgtccgacgg ggccgaagat gcgggtcccg cggggcagct
     8041 tctggtcgtt gatgaggaca gccgcgttct cgtcgaagcg gatgtagctg ccgtcgggac
     8101 gacgccgctc cttcttggtg cgcacgacga cgcacttgac gacgtcgccc ttcttgaccg
     8161 ccgcgcccgg cagggcgtcc ttgacggtgg ccacgaagat gtcgccgatc gaggcgtacc
     8221 ggcggcggga gccaccgagg accttgatga cgagcacctc tcgggctccg gagttgtcgg
     8281 ccacccggag gcggctctcc tgctggatca tcgggcgcgc tccacgatct cgaggacccg
     8341 ccagcgcttc agcttggaca gcggccgggt ctccatgacg cgcacgcggt cgccgacgtt
     8401 gaggtcgttg gtctcgtcgt ggacgtacag cttcttgtcc tgctgcatcg tcttgttgta
     8461 ccgggggtga cggacacgct cggtcacggt gacgatggcg gtgcgctcca tcttgttgga
     8521 cgtgacgatg ccctcgcgca ccttgcgggc gttgcgctct gtgccggtcg tcgcggtgtc
     8581 gtcgctcatg acgcctcgct cccgatcgcg gcttctgccg ccgcgatctc acgggcccgc
     8641 agctcggtgt tgatgcgggc gatgtccttc ttggtctgac cgatccgggc cgagttctcg
     8701 agctggccgg tggccagctg gaaccgcagc ttgaagaggc ggtccttctc ctcggcgagg
     8761 cgctcgatca gctgctcgtc gcccagggag gcgacgctgg tgtccttcgc catcagatct
     8821 cctcctcgcg ggtgatgatc ttggccttca ccggcagctt ctggatggcc cggttcagcg
     8881 caccgcgggc gatggcgtcg tcggggtagc tgagctcgaa gagcacccgt ccggggcgca
     8941 cgacggcgac ccagcgctcc gggttgccct tgccggatcc catccgggtc tcggccggct
     9001 tctgggtcac cggcttgtcg gggaagacgt tgatccacac cttgccgcca cgcttgatgt
     9061 ggcgggtcat ggcgatacgg gcggcctcga tctggcgggc ggtgatccac ccgggctcga
     9121 gggcctggat gccgtactgg ccgtgggtca cgttggtgcc gcccttggag acacccttgg
     9181 tgcggccgcg gtgctgcttg cggtgcttga ccttgcgggg catcaacatc agtcagcgtc
     9241 tcccggtcgg aagtgcgggg tctcgtggtg ctcgcgggtg gagcgttcga tctcctcctc
     9301 ctcggcgagc aggcgttcga actcggggtc cgcctcctgg accagcggct tgaggtcctc
     9361 gtcggacacc tcgggtgccg gcgcgggcga ggccggagcc gccgcagctg cgggggccac
     9421 gggcttggtg gacacaaccc ggggacgctc ctggccggcg gtctcgccga cggccatggc
     9481 ggcctccttg gtgatcttgt cctcgttggc gctcttgtag ggcaggatgt cgcccttgta
     9541 gatccagacc ttgacgccga tgcgaccggc cggggtgcgg gcctcgcgga agccgtagtc
     9601 gatgtcggcg cgcagcgtgt gcagcgggac gcgaccctcg cggtaccact cggtgcggct
     9661 catctcggag ccgccgaggc gaccggagca ctggacccgg atgccgaggg cgccggcctt
     9721 ctgggcgttc tgcacggccc gcttcatggc ccgccggaac gcgatgcggt tggcgagctg
     9781 gtcggccacg ccctgggcga tcagcgcggc gtcgacctcg ggctgcttga tctcctggat
     9841 gttcagctgg accttggggt tgccggtcag cttggtgagg tccgcgcgca gctcgtcggc
     9901 ctgggcgccg cggcgtccga tgacgatgcc gggacgggcg gtgtgcacgt cgacgcggag
     9961 tcggtcgcgg gtgcgctcga cctcgatccg gctgatggcc gcgttcggca gcttgttcat
    10021 caggtagtcg cggatcttcc agtcctcgat gaggtagtcg acgtactcct cacgggtggc
    10081 gaaccaccgg gacttccagt ccgtggtggt gccgaggcgg aagccgtagg ggttgacctt
    10141 ctggcccatc agtcctcgtc cttcttctcg gtctcgccat cgctgtcgtc ggaggcgtcg
    10201 gagtcggcgt cggccgcatc gtcggccgcc tcggcctcgg cgtcggagac gacctcgtcg
    10261 gtcgccgcct cgtcaccggc ctgctcggcg gtcgcctcgt cggtcgcggc ctcgtcgacc
    10321 gtgctttctt cgacggtgct gtcctctgcc gtgctgtcct ctgccggcgc ctcctcggcg
    10381 gcggcaccgg agcggcgctt gctgccggcc acgcgacggg cccgggccga tgcggcgtcg
    10441 ggggtggccc ggccggactc ggcctgacgg gcgcggatcc ggtcgaactc ctcgaccgag
    10501 tagcgcccca cgatgatcgt gatgtggcag gtgcgcttgc ggatgcgggt ggcccgaccg
    10561 cgggcccggg ggcgccagcg cttgagcgtg gggccctcgt ccgcgtaggc ggtggcgacg
    10621 aagagctcct cgggcgggat gtcgttgttg ttcgccgcgt tggcgacggc ggagtcgagc
    10681 accttgatga tgacgtcggc cgcgccccgc tcggagaact gcaggatggt ccgggcctcg
    10741 gccacggact tgccccggat caggtcgagc acgacccggg ccttggtggc cgacatccgg
    10801 gcgtggcggt gctgggcgcg aacgccgggc cgctcgttgg tcttgggtcc ggtcatcgcc
    10861 gacccctcgc gcccttctcc tgcccggcgt ggaacttgaa ggtccgggtg ggagcgaact
    10921 cgccgagctt gtggccgacc atcgactcgg tcacgtacac cggcacgtgc ttgcggccgt
    10981 cgtggacggc gatcgtgtgg ccgaccatct cggggatgat cgtggagcga cgcgaccagg
    11041 tcttgatcac gttcttctgg cccttctcgt tgaggtcgtc caccttcttg agcaggtggt
    11101 cgtccacgaa gggacccttc ttcaggctgc gaggcatctc agatctccct tgtcaccggc
    11161 gcgatccgcg cgtccggcga cggcgcacga gcagcttctg ggacggcttg ttcttgttgc
    11221 gggtgcggcc ctcgggcttg ccccagggcg acaccgggtg acgaccaccc gaggtcttgc
    11281 cctcgccacc accgtgcggg tggtcgacgg ggttcatggc cacaccgcgg gtctgcgggc
    11341 gcacgccctt ccagcggttg cggccggcct tgccgatctt gacgaggtcg tgctcggagt
    11401 tgccgacctc gccgacggtg gcccggcagt cgatcgggac gcggcgcatc tcggtcgagg
    11461 ggagacgcag ggtggcgtag tcgccctcct tggccacgag ctgcacgctc gagccggccg
    11521 agcgggccat cttgccgccg gcgcccggct tgaactcgac gttgtgcacg acggtgccga
    11581 ccgggatgta gcgcagcggc agggcgttgc cggagcggat ctcggcaccc tggccggact
    11641 ggaccatgtc gccgacgttg agtcccttgg gggcgaggat gtagcgcttc tcgccgtcga
    11701 ggtagtgcag cagggcgatg cgacaggtgc ggttggggtc gtactcgatg gcggcgacct
    11761 tggccggcac gccgtccttg cgccgcttga agtcgatcac gcggtactgc tgcttgtgac
    11821 cgccgccgcg gtgacgtgcg gtcttgcggc cgtggttgtt gcggccaccg gtcttggact
    11881 tcttggcgac gagggacttc tccggggtgg agcgggtgat gtcgctgaag tccgagacgg
    11941 tctggaaccg acgaccgggg ctggtgggct tgcgcttgcg aaccgccatg tcacttcacc
    12001 tcgaacaggt cgatctggtc accttcggcc agggtgacga cggcccgctt ggtgtcgggc
    12061 cggctgccga aggtgggcga acggcggttg cgccgcttct tgcccttgcg gttgagcgtg
    12121 ttgaccttct tgaccttcac gccgggccag atggcctgga ccgcgtcgcg gatctcgggc
    12181 ttggaggcgc tgggagcgac gatgaaggtg tagacgccgg tgtcgagcag cccgtaggac
    12241 ttctcggaga cgaccggccg gatgatgacg tcgcggggat ccttcacgat caggcccctt
    12301 cctcggaggt gtcggcggcg ggctcggcga cggccggagc ggacgtgggc agggtggcct
    12361 cggtgaacac gacgtggtcg ctgaccagga cgtcgtaggt gttgagctca ccggcggtga
    12421 ggacgtgcac cgacggcagg ttgcggaagc tcttccaggc ggtgacgtcg tcggggctca
    12481 gcacgacgag tgcccgaccc tcgacgccca tggcggccag cgccgcggcc gcggccttgg
    12541 tcgacggggt gtcgaagtcc caggcggaga cgaccacgac cttgtcgtcc gcagcgcggt
    12601 ccgacagggc cgagcgcagc gccaggcgct tcatcttctt gggggtcttc tggtcgtact
    12661 tgcggggctt ggggcccagg gcgacgccgc caccacgcca gatcggcgag cggctggagc
    12721 cggcgcgggc acggccggtg cccttctgct tccagggctt ggcgccgcca ccggcgacct
    12781 cggcccgggt cttggtcgac tgcgtgccgg cccgcttggc ggcgagctgg gcggtgacga
    12841 cctggtgcat gacggccacg ttgggctcga tgccgaaggt ggcgtcgtcg agctcgacgg
    12901 tgcccgtgga ggcaccggag gcgtcgtgga gagtgatcga ggccatcagg cggcaccttc
    12961 cttgctggcg gagcgcttca cggcgttgcg cagcagcacg accccgcccg tggggccggg
    13021 caccgcaccg cggaccagga tgacctcctg gtcggtgtcg gccttgacca cctcgaggtt
    13081 cagggtggtg accttggtcc cgccggtgcg accggccatg cggacgccct tgaacacgcg
    13141 ggccggggtg gcgcactggc cgatcgagcc gggcttgcgg tgcacgcggt gcgcaccgtg
    13201 cgaggcgccc tggcccttga agccgtggcg cttcatggcg ccggcgaagc ccttgccctt
    13261 gctcaccgcg gtgacgtcga tcaggtcgcc ggcggcgaac gtgtcggcgt cgagggtctg
    13321 gccgacctcg tacgcggaga tgtcgtccag tcggagctcg accaggcgga cccccgggtc
    13381 cacgtcgccc ttggcgtagt ggccggccag gggcttggtc agcttgcggg cgtcccggtg
    13441 gccgtaggtc acctgcagtg cgctgtagcc gtccttctcc ggggtccgga tctgcacgac
    13501 ccgggccggt gagacgcgca gcacggtgac cgggacgatc cggttcgact cgtcccagac
    13561 ctgggtcatg cccagcttct ctcctaccag tgcagctgtt ttcgccatcg tggttcaggt
    13621 tccttcggcg aggtccatcg acgtccgtcg tccccggttg ggaccgattc ggcgccgctc
    13681 acgcagacgc tccgcgcgga gcaggtccgg gcggagcgac gtctcgattg tgccgtgcgg
    13741 ttccccggcc gtgtgccgtg ggcctgcgcg gacgtcggtt gataacgatt ccgggagacc
    13801 gacacggggc gcacgcagcg acccgagcga cgacctggga gtcgggatca cacggtggcc
    13861 ctgtctctcc ggggaaccgg agcgcaacga gccgtccgtg acgaccgggt gagactagca
    13921 gcgccctcgg tggccgccaa gtccggatcc cccactcccg ttctgccgcc cccactcccg
    13981 ttctgtagtg cgagatccag gcgttcgcct ggatctcgca ctacagaacg gggaaatagc
    14041 gggggcgctg tcagcggagg tggctactgt gcgccgccgt gacctccacc gatcccgccg
    14101 tcgtcgccga cggcctcgtc aagagattcg gcgacaccga ggcgctgcga ggcgtcagct
    14161 tcgagatccc acgagccacc gtgctcggcg tcctcggccc caacggggcc ggcaagacca
    14221 ccgcggtgcg catcctcagc accctgctgc gcgccgacgg cggccgggcg ctcatcgacg
    14281 gcatcgacgt gggtcgccac ccccgtcagg cccgggcccg aatcgggctg accggccagt
    14341 acgccgcggt cgacgagcgg ctcaccggga tcgagaacct ccagctggtc ggacgtctgt
    14401 tccacatgcg caaggccgac gccgccgccc ggggacgcga gctgctcgac cgcttcagcc
    14461 tggaccaggc cgctgatcgt gtggtcaagg gctactccgg tggcatgcgg cgccggctcg
    14521 acatcgccat gagcctgatc gcccgaccgt cggtgctgtt cctcgacgag ccgaccacgg
    14581 gcctcgaccc acgcagccgg ctggagatgt gggacgtgat cgacggcctg gtcgccgacg
    14641 gcaccacgtt gctgctcacc acccagtacc tggacgaggc cgagcgcctg gccgacgaca
    14701 tcgtcgtcat cgacaagggc accgtgatcg cccggggcac cgaagaggag ctgaaggccc
    14761 gaagcggcgg atcgagcgtg accgtcaccc tgtcggatcc ggccgacctg ccccgcgtgc
    14821 gagggctgct cgcggacctg gttcccgccg gagcggtcga atcggtcgac gccgtggccc
    14881 ggaccgtctc ggtcccggtc acccggaccg atcacgtggt tcccaccgtg gtgcgtgctc
    14941 tcgacgccgc gggcctcgac gtcctcgacg tcgccgcaca cgaacccacc ctcgacgacg
    15001 tgttcctcca gctcaccggc cacaccgcca ccaccgacga tcccggcgtc gacgccgatt
    15061 ccggcccgga accgggccga ccggcgacat cggccgacac gccccccacc accgctggag
    15121 caccacgatg accaccaccg cccccgatcc gagcgtcgcg gcaccgtcaa cgatctcgtc
    15181 gcacacggtg ccgaccggac cgcgctggct cgccagcgac atccgggtcg aggccggacg
    15241 acacctgacc gcgacctggc gcaaccccga cctgctggtg ttcgccacga tccagccgat
    15301 catgttcgtg gtgctgttcg tctacgtgtt cggcggcgcc atcaccgttc ccggctactc
    15361 gagctaccag cagttcgtcg tgccgggcat cttcgcccag accgtcgtgt tcggatcggc
    15421 cttcaccggc atcggcatcg ccgaggacat gcagaagggc ttcatcgacc ggctccgctc
    15481 actgccgatg tacccctcgg cggtgctggt cgggcgcacc acatcggatc tggtccgcaa
    15541 cacgttcacc ttcgccatca tgatgatcgt cggcctgatc gtcgggttcc gtctggaggg
    15601 cgggttggcg aacgcggtgc tggccaccgt gctgctgctg gcgttcgcgt tcgccttcag
    15661 ctggatccag gcgctgatcg gcctgtcggt caagtcggtc gaagcggcca actccgcagg
    15721 cttcatctgg atgttcccga tgacgttcgt gtcctcggcg ttcgtcgacc cgtcgaccat
    15781 gccgggttgg ctccagccga tcgcggagca caacccgttc accgtcgtga ccaacgcggt
    15841 ccgggcgctc tacaacggca tggaccccgg caatgacctg tggatctcga tcgcctgggc
    15901 cgtcggcatc accgtcgtgt tcggagcgtt gtcgttccgc aagttctcca gcgcctcgcg
    15961 ctgagcagcc cccgccgaac ttgtagccca caggtcaccc gagcgaccgc cagctacaag
    16021 aacgcggacc gccgccgaac ttgtagccca cacgccgtcc gagcaaccgg gagctacaag
    16081 aatgcggaac gccgccgaac ttgtagccca caggtcgcct gagcaaccgc cagctacaag
    16141 ttcggcggcg ggacgcgggc gtcaggcgtt ctggatcttg atctcgatgt cgacgccggc
    16201 gggcaggtcg aggcgctgca gcgagtcgac ggtcttgggg gtcggctcga tgatgtcgat
    16261 cagccgcttg tggatccgca tctcgaagtg ctcgcgggag tccttgtcct tgtgcggcga
    16321 gcggatgacg gtgaagcggt gcttctcggt gggcagcggg atcgggcccc ggaccgacgc
    16381 ctgggtgcgg gtcacggtct cgacgatctt cttggtcgac tggtcgatga cctcgtggtc
    16441 gtaggccttc aggcggatgc ggatcttctg gtcggctgcc atggctgcac tctccggggt
    16501 tcgcgaccgc ctgggccgca cgggtctcat cgactgtcaa gaagcacgcc cggcgctccg
    16561 ggcacggcga gccagcgtag cggccgctgc gccgagggtc catccggctg gatgtggcgc
    16621 gacactgggc cgatgagcga acccatcgca gcagtcatcg acacctggca cgagttcctg
    16681 cgcggcgccc gcccggacgc cctcgacgag ctcctcgacg acgacgtcgt gttccactcg
    16741 ccgatcgtgt tcacccccca gcgaggcaag gcgatcacca cgctctacct gaccgccgcc
    16801 acccaggcgc tccccggcga cgcgccgacg ggcgacgacg ccggcgacca cccggctgac
    16861 gtcgcgatgc ccggcggcag cttccgctac accaagcagg tgctctccgg cgacaccgcc
    16921 gtgctcgagt tcgagacgac gatcgacggc ctctacgtca acggcgtcga catcatccgc
    16981 tgcaaccccg acggtcggat cgtcgagttc cgggtgatga tccggcccct gcaggccgtg
    17041 aacctggtgc accggcagat ggcggcgatg ctcgagtcga tgagctcctc gggctgaccg
    17101 ccttccgccg aggcggacgg cgcacggacc gccgaacggg acagcggacc aacaccccgg
    17161 tcgatccacc ggatcccgga cgcgacggag gggcgccgaa gcgcccctcc gtcgagctcg
    17221 tccgagcgga ccggtcaggt cacttgagga tcttggtgac gcgacccgcg cccaccgtac
    17281 gaccacc
//
LOCUS       DAOQOD010000052        17275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1166_length_17275_cov_11.220616, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000052 DAOQOD010000000
VERSION     DAOQOD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_17275_cov_11.220616"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /gene="lexA"
                     /locus_tag="PLZ72_07030"
     CDS             complement(<1..432)
                     /gene="lexA"
                     /locus_tag="PLZ72_07030"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNA36086.1"
                     /translation="MSARPTPTEPTGGPLTQRQHDVLAFIEKTVSERGYPPSVREIGE
                     GVGLASPSTVHSHLHTLQKLGYLRRDPTKPRAIEVTADTASGTTAERRPTRHVPLIGD
                     VAAGTGVLAQEQVEELLPLPADFCGDGELFMLRVRGDSMIDA"
     gene            633..1046
                     /locus_tag="PLZ72_07035"
     CDS             633..1046
                     /locus_tag="PLZ72_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNA36087.1"
                     /translation="MAAVIELHTGDRIPSAPRRPELRLLEGGRPSETSRPVVSSRTYL
                     IRRIAAVTVAALVVLVVAQILGGVGRIVASELDAAPTASAQVHVVRAGETAWAIAGRY
                     APEMDRRQAVDQLLALNGQEMIRSGQTLRLPASFG"
     gene            1119..1586
                     /gene="nrdR"
                     /locus_tag="PLZ72_07040"
     CDS             1119..1586
                     /gene="nrdR"
                     /locus_tag="PLZ72_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNA36088.1"
                     /translation="MRCPSCGGLEDRVVDSRAADDGSTIRRRRQCELCATRFTTFERI
                     GEVSLVVVKRDGRREPYERAKVRSGLESACKGRGIEPAVLDSLLSSVEDRLAVRGGEV
                     ESAQVGALVLEDLRGLDGVAAVRFASVYKGFQGPEDFEREIQLLVEPPSPPAG"
     gene            complement(1615..2349)
                     /locus_tag="PLZ72_07045"
     CDS             complement(1615..2349)
                     /locus_tag="PLZ72_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNA36089.1"
                     /translation="MHQLVPEPGPVLDVLTSLADDNRPVPADRPWVMANMVMSIDGAF
                     AVDGRSGALGTPGDRTVFHALRALADVILVGAGTARDEHYRRPVAVADAAAIRRERGQ
                     EPAARLVLVSRRGLIPDDQPFLTGDGPDPLLVHAQPLPAVVPARVDTLECPDAAGGVD
                     LPALLGHLRSNGARLVLCEGGPGLLGELHRQDLVDELFVTVSPRLVGGRSVGLLGSGP
                     ALLRARRLHRLWRDEDDALFSSYRRP"
     gene            2984..5740
                     /locus_tag="PLZ72_07050"
     CDS             2984..5740
                     /locus_tag="PLZ72_07050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNA36090.1"
                     /translation="MSITPPDTTIRLTRRFSTPGRSPYDEVAWDLRDARLTDFRSGEV
                     VFEQTRVEFPADWSVTASNIVAQKYFRGQMGSGRRERSLRQVIDRVVGRITEWGERDG
                     YFSDADEAATFSEELRWLLLHQRVSFNSPVWFNIGTPGAREQAAACFILDVEDDLASI
                     LNWYEEEGTIFKGGSGAGANLSKIRSSKELLSAGGTPSGPVSFMRGADASAGTIKSGG
                     TTRRAAKMVILDADHPDIEEFVWCKAIEERKARVLRDAGFDMDLDGADSFSVQYQNAN
                     NSVRLTDEFMQAALDGRDWELTSRTTGEVVETVPARRLLREIAEASWECADPGVQFDT
                     TINRWNTTPHAGRIDASNPCSEHLRLANSACNLASINLLSYLSEDGEFDAEAFEHTVR
                     VVVTAQDILVGGSEYPTDKIAAEARAARDIGIGYTNLGAALMALGLPYDSEDGRAWAA
                     TITGIMTGAAAVRSTELAARLGPAPLFEADADAWSGVYRMHLDAARAVDAVEGQPDLN
                     QHLVRLWTEALDRIAVVGARNAELSVAAPTGTISFMMGADTTGIEPDLGLVKHKKLVG
                     GGSLQIVNQTVPRALRRLGYDDAAVERIVAWIDENHSVVGCPDLDAAHLPVFACAMGD
                     NPISYGGHLRMMSAIQPFFSGAMSKTVNVPEDTSVDDVEQLLIDAWRSGIKCVALYRD
                     NCKVGQPLTTGKKEAAAPSAPEQVAIQVVEQVARPVRERLPRTRRSRTFEFRVADCKG
                     FVTVGEYEDGRPGELFIRVSKQGSTLAGIMDAFAISVSHGLQYGVPLRAFVGALVGMR
                     FEPAGMTDDPEVRIANSLVDYLFRRLALEYIPYEERVELGILGVSERLQPTLPGVEEA
                     ATPTVQGSDAPADPPTPAPHAASAEPIRTAAAAPLCMTCGVQMTRAGSCFVCTECGTT
                     SGCS"
     gene            5809..6165
                     /locus_tag="PLZ72_07055"
     CDS             5809..6165
                     /locus_tag="PLZ72_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36091.1"
                     /translation="MDIEQFYDADPRRRRSEEEQFGRDWFDGDGVRWELNWVADTGEV
                     YIMREPVEPGAMDAVGDTWVADMPVDLVTVEILGVVTDGAALGAALDGWTAHEGAAGS
                     LTWVRERISEVVAPTE"
     gene            complement(6252..6740)
                     /locus_tag="PLZ72_07060"
     CDS             complement(6252..6740)
                     /locus_tag="PLZ72_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNA36092.1"
                     /translation="MTRARSVAALMAVAATVALGACAAPPPVASPSGAAASSASAPDM
                     LAAVNAQRAANGKAALALCGPLIRAAETQSAAQAAQMRMHHSQLAALANSAGYFGWLS
                     LGENVAAGQTSVDQVMTAWMNSSGHKANILGSYTHAGFAQATGANGVIYWTQEFGSGG
                     SC"
     gene            6925..>7590
                     /locus_tag="PLZ72_07065"
     CDS             6925..>7590
                     /locus_tag="PLZ72_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36093.1"
                     /translation="MTDDPPPVDGSSDPVVDGPADGLHWGLDAVLGAAVLGTRAAGEV
                     AGAMASAPPVRFLGGVARWCARPLSAHGAAVRAAVVRDGLPAARDAVRRVTPAVVDAV
                     DLDSILAAVDVGALIDRVDVDGIVSRVDVEGIVSRVDVDRIVAQVDIGALLERVDIGA
                     LLDRIDLNALLAGVDLDALLAGVDLNALLAGVDLNALLSGVDLNALLAGVDLNDLLDG
                     VELD"
     assembly_gap    7591..7690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7886..8341
                     /locus_tag="PLZ72_07070"
     CDS             7886..8341
                     /locus_tag="PLZ72_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HNA36094.1"
                     /translation="MGTFDVVENFTQDIAEIGWSVAAVAGDRTGCDWAYSVGLDLTYD
                     HPELIIVGVDAPLAGAIVQAMAEKVSEGADLRDCGTVHVGPMQLRFRAVDGLFCSQGD
                     WFNLGRLVMAEMGRSWPETLQIVWADANGRYPEAPGEPDWFMRQPLLVG"
     gene            8395..9246
                     /locus_tag="PLZ72_07075"
     CDS             8395..9246
                     /locus_tag="PLZ72_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNA36095.1"
                     /translation="MFVPRPMSAPAVTSDVVSSLAAFRSATRELGPADWARPTDCPGW
                     DVHDVVAHVVTFEAFLDGEPAPAVDPEVTGVADPHVRNEIGRLNEAWVASWRGRPPRD
                     LLDGMDDLVQRRTRELAEVGDEDLAVVGWSPVGEVARSRFLQVRVLDVWFHEQDIREA
                     TGRPARSDRDVVERVVSELTHGLAFVVARRAGASDGSRVRFAVDPDDGADPIVLDIEV
                     VDGRGRVSSTETPSPTATIAAELGAFGRLVGGRRPPQEMLDAGRVSLDGDVELGRRVV
                     EHLGFMI"
     gene            9439..9918
                     /locus_tag="PLZ72_07080"
     CDS             9439..9918
                     /locus_tag="PLZ72_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNA36096.1"
                     /translation="MAFDVGDRVVYPHHGAAVIKKREKRKVNGTTTEYLVLEMAHGEL
                     TLSVPVDKADDVGMRPPIGKEEVEDLFELLGKKDVREPANWSRRFKNHQEKLKSGDVY
                     QVAEVVRNLALRDQAKGLSAGEKSMFVKARSVLVSELSFALDVSEDDALAEVEKQLQ"
     gene            10001..11032
                     /locus_tag="PLZ72_07085"
     CDS             10001..11032
                     /locus_tag="PLZ72_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA36097.1"
                     /translation="MSTPSSDLRVLQVLGHNDRDGVGQASLELHRELARRGMQVRTLA
                     LAPGREAGLSAVVPTMSPSARSLAAHTQLRREQRWADVVILRGQVPAAAAGLSRMSGA
                     PPTVLALTDEPEVWSDVPIPSRVMRLLGRAAAVVVTSPSQTASADRWHMDPADVHVIP
                     YGVTTTDVSDSRRVASRAALGLPADGVVAHLTRPAADDDGLRARLEGLGVGITVIEPR
                     DDRIVHFGAPEPEDTEELAVAAADVVVSTGPETSGPPWELLRGAAWGRAVIVDRWPGR
                     APELRGALAELLDDSTGWTDLGDAITAGRAEIERRGSAARRRVAERFSMPTVGAAWAD
                     LLGSLSDRR"
     gene            11065..11823
                     /locus_tag="PLZ72_07090"
     CDS             11065..11823
                     /locus_tag="PLZ72_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36098.1"
                     /translation="MPRHRSAPVILAILAVACLSVSACLPQERGASREVDDGVEAPRR
                     PPVFPTTGPVPRELLIDGLLYDFTARPTSLDLWGPPVAEARCAATRITDTLGPPRLSE
                     LGYRPATSGASLNDIDLTDTERELVAAQFTSCVDMREAIAAVLMGDGHMEAKEATCLA
                     DGLVQQGVSDQFARAWAFGRTVNPAEGDGKMASAFMSLATVCLPADAFRWYDTNLPGD
                     DQVQGSGNGSGTTTSTVPGTEDGLQSRIGSTSTP"
     gene            11845..12921
                     /gene="lgt"
                     /locus_tag="PLZ72_07095"
     CDS             11845..12921
                     /gene="lgt"
                     /locus_tag="PLZ72_07095"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNA36099.1"
                     /translation="MTATVLSAAGPLRVGSTVASIPSPPFRDLALGPLSFRMYGLMIA
                     LGVIAAVWIARRRYSSYGGDPEDITTIALVAVPAGLVGARLYHVITDWPDLYSDGRWW
                     PKAFMIWQGGLGIPGGVLLGAIAGVVACRVMGISWRIVGDAVAPAIPVAQAIGRLGNW
                     FNQEVFGRPTDLPWGLRIDPQFRPRDYLTSPTFHPTFLYEGLWNLGLAGLIVWASGRL
                     VLRRGRWLAVYVAGYGLGRLWVEALRSDHANTVLGLRVNIWTSLIALAGGLLWLFWRG
                     SPVDREATERLRSGTTMAQIIDGDGVRAPLVVPPAAGPGSADGPGSADGPGSDDESDP
                     SDGQDPSVDAVPSDDPAQPGGAAE"
     gene            complement(12897..13721)
                     /locus_tag="PLZ72_07100"
     CDS             complement(12897..13721)
                     /locus_tag="PLZ72_07100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA36100.1"
                     /translation="MTVLGAIDGWGASFAAAAVVRGSSPVATHGDGSRVVRVASITKL
                     LTAWAALIAVEEGATTLDTPLGPPGSTVRHLLAHAGGYDFDSDAVLAAPGTRRIYSNT
                     GYELLARHIGDATGFEFADYMAEAVLAPLGMGSSELRGSAARDLWSDVEDLLRFAAET
                     RAPTLLHPSTVATALAPAFAELDGVLPGWGPQRRCWWGLGPELKGDKDPHWSGSTASP
                     NTYGHFGGSGTFLWVDPAADLTCVVLTDREFGDWAVRAWPGFSERVRSTYSAAPPG"
     gene            complement(13718..14533)
                     /locus_tag="PLZ72_07105"
     CDS             complement(13718..14533)
                     /locus_tag="PLZ72_07105"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNA36101.1"
                     /translation="MAIDVAAHITEIDRHGFTRIRGVLDSGAVAELLSDLGRIETEEG
                     IVPADNDFEGTRTRRAYNLLRHGPSWEAVAVHPDVLAVVEGVLDPGCLLSSLSSIRIE
                     PGETDQPLHADDQLIPLTPPHPPVVCNTMWALTEFTADNGATRVVRDSHRWGKPTYGG
                     DHPTEPAIMDPGDVLVWNGSLWHAGGANRTDRDRVGIAVNYCAGWIRQQENQQLGLAP
                     DRVARFPRRLQELVGYSVYQGLIGHIERRSPIDLLADSTSNPDAGTTMVWDRT"
     gene            14699..15853
                     /locus_tag="PLZ72_07110"
     CDS             14699..15853
                     /locus_tag="PLZ72_07110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA36102.1"
                     /translation="MTTTFANLGIAADICHALQERGITDAFPIQEKTIPDILAGRDVC
                     GKAKTGSGKTLAFGLPLMQMLPRAEPGRPTGLALVPTRELATQVRDELVAASHAGGVR
                     IAAIYGGDPIEKQIKALKNGVELAVCTPGRAIDLIERGDLSVADVRHVVIDEADRMAD
                     MGFLPQVEWILRNVEGDHQTLLFSATLDGVVDGLVRRYQHDPTRHEVASKAVNVEQMT
                     HRFLLVHEMDKVKVAASIIRNAQRTMVFSATKHGADRLARKLEDEGVDAAPIHGDLRQ
                     KFREKALSDFSHGRIRALVATDVAARGIHVDDIDVVIHYDPPSDHKTYVHRSGRTARA
                     GESGVVVSLVLYNQELEIKRLQKRLGLDIPIVEIFSNDPRLADVADWPED"
     gene            15941..>17275
                     /locus_tag="PLZ72_07115"
     CDS             15941..>17275
                     /locus_tag="PLZ72_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNA36103.1"
                     /translation="MADHARGLTVDRRRELGSHATPEALALHLVELALRHLHRLPQRV
                     LDPSCGAGSFLLAAADALVRRGADPAEVVEQRLEGWDVDPEAVAHCREALRRWAAAHG
                     VRRPVDVRVVELDALDPTAGPAGSVDLVVGNPPFLSQRTLDTARDVARREQVDARFGP
                     LGPYVDEAAVFLLVAAEMLSPGGVAVMVQPRSTLSARDAGAVRDRLLEVAAPVAVWAD
                     DGRHFDAEVDVWAPVLRRGVDGDRGEEVEHGVEVHWGTAADAVDRPRPEPGQSWGPLL
                     ATALGVPEIAPAGEMAPAGAAGHRAIGDVATATAGFRDEFYALSAAARSLDEPGWGPQ
                     LPPLVTVGMIDVGRLDRRRPRRLGGRLVADPRLDVDSLQTDAPAVARWARKRQVPKVL
                     VATQTRVLEAVADLDRQMVPVTPTVSVEPTGAVGVGPQELLAAVCAPPSAARL"
BASE COUNT         2357 a         5984 c         6270 g         2564 t
ORIGIN      
        1 ggcgtcgatc atggagtcgc cccggacccg cagcatgaac agctcgccgt cgccgcagaa
       61 gtcggccggc agcgggagga gttcctcgac ctgctcctgg gcgagcaccc cggtcccggc
      121 cgcgacgtcg ccgatcaggg ggacgtgacg ggtcggacgg cgttcggccg tggtcccgct
      181 ggcggtgtcg gcggtgacct cgatcgcccg gggcttggtg gggtcgcggc gcaggtatcc
      241 cagcttctgc agggtgtgga ggtggctgtg cacggtcgac ggggaggcaa ggccgacccc
      301 ctcgccgatc tcccgcacag acggtgggta gccgcgctcg ctgacggtct tctcgatgaa
      361 ggcgagcacg tcgtgctgac gctgcgtcag gggcccgccg gtgggctcgg tgggggtggg
      421 acgtgcgctc atgggatcct cccggtcgaa caggtgtttg gaccgtagca cgtcggggag
      481 cgcaagtggt ggaacacccg ttccccgaac cgatgttcga tcaggtcctt gacggcgaac
      541 agctgttcgg gcaaggtgtg gcgaacgggt gttcccctcg gttccgggga acgccgatcg
      601 gcactgtcac acctcgtctg gagactctcc ccatggctgc agtcatcgaa ctccacaccg
      661 gcgaccggat ccccagcgct ccccgccgtc ccgagctacg ccttctcgaa ggtggtcgtc
      721 cgagcgagac gagccgaccg gtcgtgtcgt cccgcacgta cctgatccgc cggatcgcgg
      781 cagtgacggt ggccgctctc gtcgtcctcg tcgtggcgca gatcctgggc ggtgtcgggc
      841 ggatcgtcgc gtccgagctc gacgctgcgc cgacggcctc cgcccaggtc cacgtcgtgc
      901 gagccgggga gacggcgtgg gccatcgcag gccgctatgc ccccgagatg gaccgtcgcc
      961 aggctgtgga ccagctcctg gcgctcaacg gccaggagat gatccgttcg ggtcagaccc
     1021 tccgccttcc ggcatcgttc gggtgacacg ctcgtcgacc gctgtcctac atggtgggag
     1081 atcgtcgccc aggggtaggc gccggtaggg tcggctccgt gcgttgcccc agctgtggtg
     1141 gactcgagga ccgggtcgtg gactcccgcg ccgcggacga cggcagcacc atccgacgtc
     1201 gccgtcagtg cgagctgtgc gcgacgcggt tcacgacgtt cgagcgcatc ggcgaggtgt
     1261 cgttggtcgt ggtcaagcgc gacggacggc gtgaacccta cgaacgggcc aaggtgcgct
     1321 ccggtctgga gtctgcgtgc aaggggcggg ggatcgaacc cgcggtcctg gactcgctgc
     1381 tgtcgtcggt cgaggaccgc ctcgcggtgc gcggtggaga ggtcgagagc gcccaggtcg
     1441 gcgcgttggt gctcgaggac ctgcggggcc tcgacggcgt cgccgcggtc aggttcgcgt
     1501 cggtgtacaa gggcttccag ggcccggagg acttcgagcg cgagatccag ctcctggtcg
     1561 agcccccgtc cccaccggcc ggatgagcgg tcggccgccg agcggagtgc ccgctcaggg
     1621 ccgccggtag ctcgagaaca gcgcgtcgtc ctcgtcgcgc cacagcctgt ggagtcggcg
     1681 agctcgcagc agcgccggcc ccgagccgag cagaccgacc gagcggcctc cgaccagccg
     1741 cggcgagacc gtcacgaaca gctcgtcgac gaggtcctgc cggtggagct ctccgagcag
     1801 accgggcccg ccctcgcaca gcaccagacg tgccccgttc gagcgaagat gacccaggag
     1861 agcggggagg tcgactccac ctgcagcatc ggggcattcg agcgtgtcga cccgggcggg
     1921 cacgacggcg gggagcggct gggcatggac cagcagcggg tcggggccgt caccggtcag
     1981 gaacggctgg tcgtcgggga tgagcccgcg gcggctgacc agaaccagcc gtgcggctgg
     2041 ctcctggccc cgctccctgc ggatcgcggc tgcgtcggcc accgcgaccg gtcgccggta
     2101 gtgctcgtcg cgggctgtcc ccgcgccgac caggatcacg tcggcgaggg ctctcagcgc
     2161 gtggaagacc gtgcggtcac cgggggtccc cagcgccccg gaccgaccgt cgaccgcgaa
     2221 ggcgccatcg atgctcatga ccatgttcgc catcacccac ggacgatcag cggggaccgg
     2281 ccggttgtcg tcggccagtg aggtcaggac gtcgaggacg gggccgggtt cgggaacgag
     2341 ctggtgcacg gaccgaccct acgactcgga ccgcacctgt cccgagccat ccggcgagcg
     2401 tggggccggg agggggcccg gatgatcgga ccctctacgg ggccagcggc cgcccacggc
     2461 ccccaccgcg agcgaacgcg gtggacatct gtgggtaacc gcgccggaaa tccacatgat
     2521 gttccccttt cgacccacag gtgacccttc ctagggtgtg catctgcggg ccgtggtgcg
     2581 ggcggccgga atggtggctg acgccttccc gatccgtcgg gtcggcgact cctgggggtc
     2641 tgggccccga cgaccgcggt cgtcggttgt cctgtccggc ctccgcccgg tcgtccgtgc
     2701 ccgaccgtgc gcgtcggttg gttccggctg gcggcgggtg gtgactgcgt cccggcgggg
     2761 cgcaggtggc atggtcggat atgtcacacc gagggtgccc ggctccgtcc tacccagggt
     2821 gcagcatggt cgtcatgtcc cgccagcagc tcggcacgga acgtggatcg ggtcgcggcg
     2881 accgcacgac ggtcagcgac cgcctggccg gcgggccgcc cgacgccgtg catgtcggac
     2941 ggtcgacacc tcagacaacg gacaacggcg caaggagcgt tctgtgagca tcacccctcc
     3001 cgacaccacc attcggctca cccggcgctt cagcacaccg gggcgatcgc cgtacgacga
     3061 ggtggcgtgg gacctgcgtg acgcccgatt gaccgacttc cgcagcggag aggtcgtgtt
     3121 cgagcagaca cgggtcgagt tcccggccga ctggtcggtc accgcctcga acatcgtcgc
     3181 ccagaagtac ttccgtgggc agatgggcag cggccgtcgg gagcgttccc ttcggcaggt
     3241 gatcgatcgg gtcgtcggtc ggatcaccga gtggggggag cgtgacggct acttctccga
     3301 cgcggacgag gcggcgacgt tctccgagga gctgcggtgg ctgcttctgc accagcgggt
     3361 gtcgttcaac tctccggtct ggttcaacat cggcaccccc ggcgcccggg agcaggcggc
     3421 ggcgtgcttc atcctggacg tcgaggacga cctggcgtcg atcctcaact ggtacgagga
     3481 ggagggtacg atcttcaagg gtggctcggg ggcgggtgcc aacctgtcca agatccgttc
     3541 gtccaaggag ctgctgagcg cgggtgggac cccgtcgggt ccggtcagct tcatgcgggg
     3601 cgccgatgcg tccgccggca ccatcaagag cggcggaacc actcgacggg ccgccaagat
     3661 ggtcatcctc gacgcagatc accccgacat cgaggagttc gtgtggtgca aggcgatcga
     3721 ggagcgcaag gcccgggttc tgcgcgacgc cggcttcgac atggacctcg acggcgccga
     3781 ctcgttctcg gtccagtacc agaacgccaa caactcggtg cgcctcaccg acgagttcat
     3841 gcaggccgcg ctcgacggga gagactggga gctcacctcg cgcacgaccg gtgaggtggt
     3901 ggagacggtc ccggcccgtc gcctcctgcg cgagatcgcc gaggcgtcct gggagtgcgc
     3961 agatcccggc gtgcagttcg acacgacgat caaccgctgg aacacgacgc cgcacgccgg
     4021 tcggatcgac gccagcaacc cgtgcagcga gcacctgcgc ctggccaaca gcgcgtgcaa
     4081 cctggccagc atcaacctgc tgtcgtacct gtccgaagac ggcgagttcg acgccgaggc
     4141 cttcgagcac accgtccgcg tggtcgtgac cgcgcaggac atcctggtcg ggggatccga
     4201 gtacccgacc gacaagatcg ccgccgaggc ccgcgctgcc cgcgacatcg gcatcggcta
     4261 caccaacctc ggcgcggcgc tcatggcgtt gggcctgccc tacgactccg aggacggacg
     4321 ggcgtgggcg gcgacgatca ccggcatcat gaccggggcg gcggcggtgc gctcgaccga
     4381 gctcgccgcc cggctcggcc cggctccgct gttcgaggcg gacgcagacg cctggagcgg
     4441 cgtctaccgc atgcacctcg acgcggcgcg cgccgtcgac gcggtcgagg gccagccgga
     4501 tctcaaccag cacctggtgc ggctgtggac cgaagccctc gatcggatcg ccgtcgtcgg
     4561 ggcccgcaac gcggagctgt cggtggccgc tcccaccggg acgatctcgt tcatgatggg
     4621 tgccgacacg accggcatcg aacccgatct gggactggtg aagcacaaga agctcgtcgg
     4681 cggcggatcg ctgcagatcg tcaaccagac cgtgccccgg gccttgcgcc gactcggcta
     4741 cgacgacgcc gccgtcgagc ggatcgtggc atggatcgac gagaaccaca gcgtcgtcgg
     4801 ctgcccggac ctggacgccg cacacctgcc ggtcttcgcc tgcgccatgg gcgacaaccc
     4861 gatctcctac ggcgggcacc tgcggatgat gtcggccatc cagccgttct tctcgggtgc
     4921 catgtccaag acggtcaacg tccccgagga cacctcggtc gacgacgtcg agcagctcct
     4981 gatcgacgcg tggcgcagtg gcatcaagtg cgtcgcgctc taccgggaca actgcaaggt
     5041 cggccagccc ctcaccaccg gcaagaagga agccgctgcc ccctccgcac cggagcaggt
     5101 cgccatccag gtcgtcgagc aggtcgcccg gccggtccgg gaacggctgc cccgtacccg
     5161 ccggtcccgc accttcgagt tccgcgtggc ggactgcaag ggcttcgtga ccgtcggcga
     5221 gtacgaggac ggccgtcccg gtgagctgtt catccgggtc tccaagcagg gatcgacgct
     5281 ggcggggatc atggacgcct tcgccatctc ggtcagccac ggccttcagt acggcgtgcc
     5341 gttgcgggcc ttcgtcggcg ccctggtcgg gatgcgcttc gaaccggcgg gcatgaccga
     5401 cgaccccgag gtccggatcg ccaactcgct ggtcgactac ctgttccggc ggctcgctct
     5461 cgagtacatc ccctacgagg agcgcgtcga gctcgggatc ctgggcgtgt ctgaacggct
     5521 gcagcccacc ctgccgggcg tcgaggaggc cgccacgccg accgttcagg gctccgacgc
     5581 gccagccgat ccgcctaccc ctgcacccca tgctgcgtcg gccgaaccca tccggactgc
     5641 cgcggcagct ccgttgtgca tgacgtgcgg ggtccagatg acccgggccg gatcctgctt
     5701 cgtctgcacc gagtgcggca ccaccagcgg ctgcagctga catgggtgga cacgtggcga
     5761 gccgacgtga ggccggctgg tcggtcggtg agtagggtcg gggttcacat ggacatcgaa
     5821 cagttctacg acgccgatcc gcgccgccgg cgctccgagg aggagcagtt cggtcgcgac
     5881 tggttcgacg gtgacggcgt gcgctgggag ctcaactggg tcgccgacac cggcgaggtc
     5941 tacatcatgc gcgagccggt ggaacccggc gccatggacg ccgtgggcga cacgtgggtg
     6001 gccgacatgc ccgtggacct ggtcaccgtg gagatcctcg gcgtggtgac ggacggggcc
     6061 gccctcggcg ccgcgctcga cgggtggacc gcccacgagg gcgccgcggg ttcgttgacg
     6121 tgggttcggg agcggatctc cgaggtggtc gccccgaccg agtgagcaag cgggcccttc
     6181 tgcttctgct cacccgtcgg gctggattgc tcgtcgatgt cgggcgtcct gagcgcgtga
     6241 gcgggtccag gtcagcagct gccgccggag ccgaactcct gggtccagta gatgaccccg
     6301 ttggcgccgg tggcctgggc gaacccggcg tgggtgtagc tcccgaggat gttggccttg
     6361 tggcccgatg agttcatcca ggcggtcatg acctggtcga ccgacgtctg accggcggcc
     6421 acgttctcac ccaggctgag ccagccgaag taccccgcgc tgttggcgag ggccgccagt
     6481 tgcgagtggt gcatccgcat ctgcgccgcc tgcgcagccg actgggtctc ggcggcccgg
     6541 atcagcggtc cgcacaacgc gagagctgcc ttgccattgg cggctcgctg ggcgttcacc
     6601 gccgcgagca tgtcgggggc cgacgccgat gacgcggcgg ctccggaggg agatgcgacc
     6661 gggggcgggg cggcgcacgc ccccagggcg acggtggcgg cgaccgccat cagggcggcg
     6721 acggatcggg cgcgggtcat cggggtcctc ccgggatgct ggtgctgctg ctggtgctgt
     6781 tgctgttcgg gcgacgcgat cgatcgcggc gcccgacagg tggtcggtgc gttccgtggc
     6841 ggacttgagc ccgaacgcgg tcgagtgcgg gaggtcggga ggaactgccc acggaggtcg
     6901 gatcatcggc aatgatctgg cgtggtgacc gacgatcctc cccctgtcga cgggtcttct
     6961 gatccggtcg tcgatggccc cgccgacggc ctccactggg gtctcgacgc cgtcctcggc
     7021 gctgcggtcc tgggaaccag ggctgcgggt gaggtcgcgg gggcgatggc ttccgctccg
     7081 ccggtccggt tcctgggagg ggtggcccgg tggtgtgccc ggccgctgag tgcccatggc
     7141 gcagcggttc gggcggccgt ggtgcgcgac ggactgccgg cggcccggga cgccgtacgg
     7201 cgtgtcacgc ctgcggtggt cgacgcggtc gatctcgact cgatcctcgc cgcggtagac
     7261 gtgggggccc tgatagaccg ggtggacgtc gacggcatcg tgtcacgggt ggatgtcgag
     7321 ggcatcgtgt cccgcgtcga tgtcgatcgc atcgtcgctc aggtggacat cggcgcgctc
     7381 ctcgaacggg tcgacatcgg cgcgctgctc gaccggatcg acctgaacgc gctgctggcg
     7441 ggggtggacc tggacgcgct gctcgccggt gtggacctca acgcgctgct cgccggggtc
     7501 gacctcaacg ccctgttgag cggcgtcgac ctgaacgcgc tgctggccgg ggtggatctg
     7561 aacgacctcc tcgacggcgt cgagctcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn gatcggcgac cagtccggca gccggagtgg aggccgtcgg cgcggcagtg
     7741 gtccgttcga cccggttgcg gttgcccgtc gtgggcccca cgtccggggt gtccgggggt
     7801 tctgcccacg ctccgtccgg caccggtcac aggttggcgg aatcgggtgc agacaaccgt
     7861 ggtcggcttc gtcacactgc gggccatggg aaccttcgac gtcgtcgaga acttcacgca
     7921 ggacatcgcc gagatcggct ggtcggtcgc cgcggtcgcc ggggaccgga ccgggtgcga
     7981 ctgggcctac agcgtcgggc tggacctcac ctacgaccac cccgagctga tcatcgtggg
     8041 ggtcgacgcc cccctggccg gagcgatcgt gcaggccatg gcggagaagg tctccgaggg
     8101 tgccgacctg cgcgactgcg gaaccgtcca cgtcgggccg atgcagctgc ggttccgggc
     8161 tgtcgacggg ctgttctgca gccagggcga ctggttcaac ctgggtcggc tggtgatggc
     8221 cgagatgggt cgcagctggc cggagaccct ccagatcgtg tgggccgacg cgaacggccg
     8281 ctaccccgag gcgcccggcg aacccgactg gttcatgcgc cagcccctgc tggtcggctg
     8341 acgtcgatca catccggccc cgacgggcga tcccgggtgt ggcaggctcc ggcggtgttc
     8401 gtgccccgac cgatgtccgc cccggcggtg acctccgacg tggtctcctc gctcgccgcc
     8461 ttccgcagcg cgacgaggga gctcggaccc gccgactggg cacggcccac cgactgcccg
     8521 gggtgggacg tgcacgacgt ggtcgcccac gtcgtgacct tcgaggcgtt cctcgacggt
     8581 gagccggcgc ccgcggtcga cccggaagtg accggcgtcg ccgaccccca cgtccgcaac
     8641 gagatcggcc gcctcaacga ggcgtgggtc gcctcctggc gggggcgacc tccccgggat
     8701 ctgctcgacg ggatggacga cctcgtgcag cgccgcaccc gtgagctggc cgaggtcggc
     8761 gacgaggacc tggccgtcgt gggttggagc ccggtcggcg aggtggcccg ctcgcggttc
     8821 ctgcaggtcc gcgtcctcga cgtctggttc cacgagcagg acatccggga ggcgaccggc
     8881 cgcccggccc gctccgaccg cgacgtcgtg gagcgggtcg tctccgagct cacccacggc
     8941 ctcgcgttcg tggtcgctcg gcgggccggc gcgtcggacg gatcccgtgt gcgcttcgcc
     9001 gtcgaccccg acgacggcgc agatccgatc gtgctcgaca tcgaggtggt cgacggacgt
     9061 gggagggtgt cgtcgacgga gacgccgtcg cccaccgcga cgatcgccgc ggagctcgga
     9121 gcgttcggtc gtctggtcgg cgggcgtcgc ccgccacagg agatgctcga cgccggccgg
     9181 gtgtcgctcg acggcgacgt cgagctcggt cgacgggtgg tcgagcacct cgggttcatg
     9241 atctgagcgc cgggacgtcc tcgcgtgtga cgcccgggcg ctgagctctc aggccccgcc
     9301 gatcgcgtcg accggggtcc aggaccggtg gtatcatcgt ccgttcgtca ggtccggctc
     9361 gttgcggagc aggggaccgg atccgaccgg cgggcacccg tcccgaccgc aaccctcgac
     9421 cccgtgagga aacccccagt ggcattcgac gtcggcgacc gcgttgtcta cccgcaccac
     9481 ggtgcggcgg tgatcaagaa gcgcgagaag cgcaaggtca acggcaccac caccgagtac
     9541 ctggtgctcg agatggccca cggcgagctc accctgtcgg tcccggtcga caaggccgac
     9601 gacgtcggca tgcgcccgcc gatcggcaag gaggaggtcg aggacctgtt cgagctcctc
     9661 ggcaagaagg acgtccgaga gcccgccaac tggagccggc ggttcaagaa ccaccaggag
     9721 aagctcaagt cgggcgacgt gtaccaggtg gccgaggtcg tccggaacct ggcgctgcgc
     9781 gaccaggcca agggcctctc cgcgggtgag aagtcgatgt tcgtcaaggc ccgcagcgtc
     9841 ctggtgtcgg agctgagctt cgcgctcgac gtcagcgagg acgacgccct ggccgaggtg
     9901 gagaagcagc tgcagtgagc tgaccgccgg tgcgccaccc cgcgccggcc gttctcctcg
     9961 agctgcgccc gcccagccgc cgacctgccg tcccccgccg gtgagcaccc cttcgtcgga
    10021 cctgcgggtc ctgcaggtac tcggacacaa cgaccgcgac ggcgtcgggc aggcgtcgtt
    10081 ggagctgcac cgggagctgg cccgacgggg gatgcaggtt cggaccctgg cgctggcccc
    10141 cggtcgtgag gccgggctgt ccgcagtggt tcccaccatg agcccctcgg cccgctcgtt
    10201 ggcggcgcac acgcagctcc gccgggagca gcggtgggcc gacgtggtga tcctgagggg
    10261 ccaggtccct gccgcggcgg cgggcctgtc gcggatgtcg ggcgctcctc cgacggtgct
    10321 ggcgctgacc gacgaacccg aggtctggtc cgacgtgccg atcccgtccc gggtgatgcg
    10381 cctcctcgga cgtgccgcgg cggtcgtggt gacctcgccg tcgcagaccg cctccgccga
    10441 ccgctggcac atggaccccg ccgacgtcca cgtcatcccc tacggcgtca ccacgaccga
    10501 cgtcagcgac tcccggcgcg tggcgtcacg tgccgctctg ggactcccgg ccgatggtgt
    10561 cgtggcccac ctgacccgac ccgcagccga cgacgatggg ctgcgggccc gcctggaagg
    10621 actcggcgtg gggatcacgg tgatcgaacc ccgagacgac cggatcgtgc acttcggagc
    10681 tccggagccg gaggacaccg aggagctcgc cgtggcggcc gctgacgtgg tcgtctcgac
    10741 cggtccggag acctcggggc cgccgtggga gctcctgcgg ggcgcggcat ggggtcgggc
    10801 ggtgatcgtc gaccggtggc ccgggcgggc gccggagctg cggggagcgc tcgccgagct
    10861 gttggacgac tccaccggct ggaccgacct cggcgacgcg atcacggccg gccgtgccga
    10921 gatcgaacgt cgggggagcg ccgcccgccg tcgggtggcc gagcggttct cgatgccgac
    10981 ggtgggcgcc gcgtgggccg atctgctggg ttcgctcagc gaccgccgat gaagcggaca
    11041 acaccgccgc taccctccgg tggcgtgccg cgccaccgct cagctcccgt gatcctggcg
    11101 attctggctg tggcgtgcct gtcggtcagc gcgtgtcttc cccaggagcg gggggcgtca
    11161 cgtgaggtcg acgacggcgt cgaggcaccc cgacgtccgc cggtgttccc cacgacgggt
    11221 ccggtccccc gggagctgtt gatcgacggc ctcctctacg acttcaccgc tcggccgacg
    11281 agcctggacc tgtggggccc gccggtcgcc gaggcccgct gcgcggccac ccgcatcacc
    11341 gacacgctgg gcccaccgcg cctgtcggag ctggggtacc ggccggcgac cagcggggcc
    11401 tcgctcaacg acatcgatct caccgacacc gaacgggagc tggtcgcggc gcagttcacg
    11461 tcgtgcgtcg acatgcgcga ggcgatcgcc gcggtgctga tgggcgacgg gcacatggag
    11521 gccaaggagg ccacgtgcct ggccgacggg ctggtgcagc agggtgtctc cgaccagttc
    11581 gcccgggcgt gggcgttcgg acgaaccgtg aacccggcag agggcgacgg gaagatggcg
    11641 tcggcgttca tgtcgctggc gacggtgtgc ctgcccgccg acgcgttccg gtggtacgac
    11701 accaacctgc ccggcgacga ccaggtgcag ggcagcggca acggcagcgg gacgaccacc
    11761 tccaccgttc cggggaccga ggacggcctg cagagccgca tcggctccac ctccactccc
    11821 tgagcggcca tcatgggccc cgacatgact gccaccgtgc tctccgctgc gggcccgtta
    11881 cgggtcggct ccaccgtcgc ctcgatcccc agtccgccgt tccgcgacct ggctctcggg
    11941 ccgctgtcgt tccgcatgta cggcctgatg atcgccctcg gcgtgatcgc cgcggtgtgg
    12001 atcgcccgac ggcggtactc gagctacggc ggcgatcccg aggacatcac cacgatcgcg
    12061 ctggtcgccg tacctgcggg cctggtcggg gcccggctct accacgtcat caccgactgg
    12121 ccggacctgt actccgacgg ccggtggtgg ccgaaggcgt tcatgatctg gcagggcggg
    12181 ctgggcatcc ccggaggggt cctcctggga gcgatcgccg gcgtggtggc gtgtcgggtg
    12241 atggggatca gctggcggat cgtcggcgat gccgtggctc cggccatccc ggtcgcccag
    12301 gcgatcggac ggttgggcaa ctggttcaac caggaggtct tcggccgacc gaccgacctg
    12361 ccgtggggtc tgcgcatcga tccgcagttc cggccccggg actacctgac gtcgcccacg
    12421 ttccacccga cgttcctgta cgaggggctc tggaacctcg gtctggccgg gctgatcgtc
    12481 tgggccagcg gacggctggt cctgcgacgg ggtcggtggc tcgcggtgta cgtggcgggc
    12541 tacggcctcg gccggctctg ggtcgaggcg ctgcgctcgg atcacgccaa cacggtcctg
    12601 ggtttgcggg tcaacatctg gacgtcgctg atcgccctcg ccggcgggct gctgtggctg
    12661 ttctggcggg ggagcccggt cgaccgcgag gccaccgaac gcctgcgttc gggcacgacc
    12721 atggcccaga tcatcgacgg cgacggagtc cgggctccgc tggtggtccc tccggccgcc
    12781 ggtcctggct ccgctgacgg tcctggctcc gctgacggcc ctggctccga tgacgagtcc
    12841 gacccctccg acgggcagga tccatccgtc gacgccgttc cgtcggacga ccccgctcaa
    12901 ccgggtggag cagccgagta ggtgctgcgg acccgctccg agaatccggg ccatgccctg
    12961 accgcccagt ccccgaactc gcggtcggtc agcaccacgc aggtcaggtc ggccgcgggg
    13021 tcgacccaca ggaaggttcc gctgcctccg aagtggccgt aggtgttcgg cgaggccgtg
    13081 gatccactcc agtgcgggtc cttgtcgccc ttcagctccg gtcccagacc ccaccagcac
    13141 cgacgttggg gcccccagcc cggcaggacc ccgtcgagct cggcgaaggc cggggcgagc
    13201 gcggtggcga cggtgctcgg atgcagcagc gtcggagccc gggtctcggc cgcgaaccgc
    13261 aggaggtcct cgacgtcgga ccacaggtcg cgggccgcgg atccccgcag ctcgctcgag
    13321 cccatgccca gcggggcgag caccgcctcg gccatgtagt cggcgaactc gaacccggtc
    13381 gcgtccccga tgtgtcgggc cagcagctcg tagccggtgt tggagtagat gcgacgggtg
    13441 ccgggagcag ccaggaccgc atcggagtcg aagtcgtagc cgccggcgtg ggcgaggagg
    13501 tgccggacgg tgctgccggg tgggccgagc ggggtgtcca gcgtggtggc gccctcctcg
    13561 accgcgatca gcgcggccca ggcggtgagc agcttggtga tcgacgcgac tctcacgacg
    13621 cgggagccgt cgccgtgcgt cgcgaccggt gaggagccgc ggaccaccgc cgcggcggcg
    13681 aacgaggctc cccagccgtc gatcgctccg agcacggtca tgtcctgtcc cagaccatcg
    13741 tggtaccggc gtccgggttc gacgtgctgt ccgcgaggag gtcgatgggc gatcggcgct
    13801 cgatgtggcc gatcagcccc tggtacaccg agtagccgac cagctcctgc aggcggcggg
    13861 ggaaccgcgc gacccggtcc ggtgccaggc ccagctgctg gttctcctgc tggcggatcc
    13921 agccggcgca gtagttcacg gcgatgccga cgcggtcccg gtcggtgcgg ttggcgccgc
    13981 cggcgtgcca caggctgccg ttccacacga ggacgtcgcc ggggtccatg atcgccggct
    14041 cggtcgggtg gtccccgccg taggtcggct ttccccaccg gtggctgtcg cgcacgaccc
    14101 gcgtggcccc gttgtcggcg gtgaattcgg tgagcgccca catcgtgttg cacaccaccg
    14161 gtgggtgggg tggggtcagg gggatgagct ggtcgtcggc gtggagcggt tggtcggtct
    14221 cgccgggttc gatgcggatc gacgacagac tcgacagcag gcaacccggg tcgagaaccc
    14281 cctcgaccac ggcgaggacg tcgggatgga ccgcgacggc ctcccacgac ggaccgtgac
    14341 gcagcaggtt gtacgcccgc cgggtccggg tgccctcgaa gtcgttgtcg gcgggaacga
    14401 tcccctcctc ggtctcgatc cggcccaggt cggagaggag ttcggcgacc gctccggagt
    14461 cgagcacgcc ccggatgcgc gtgaacccgt gccggtcgat ctccgtgatg tgcgccgcca
    14521 cgtcgatcgc catctcggtg cctctctctc gatctgttcg gccgccccgc ccgaccggtc
    14581 cgcaccgtcg cggatggtcc gctgcggtcg gagggtggtg ctctggacac gggtcagggt
    14641 cggccgctcc ggtgctccgc actacgatga cgtccatcat cacggatccg aagcggaaat
    14701 gacgacaacc ttcgccaacc tcggcatcgc cgccgacatc tgccacgccc tccaggagcg
    14761 aggcatcacc gacgccttcc ccatccagga gaagacgatc cccgacatcc tcgccggccg
    14821 cgacgtctgc gggaaggcca agaccggttc gggcaagacc ctggccttcg gtctgcccct
    14881 gatgcagatg ctgccccggg cagagccggg gcggccgacc ggcctggctc tggtgcccac
    14941 ccgcgagctc gccacgcagg ttcgcgacga gctggtcgcg gcatctcacg ccgggggagt
    15001 tcgcatcgcg gcgatctacg gcggcgaccc catcgagaag cagatcaagg ccctcaagaa
    15061 cggtgtcgag ctggcggtgt gcacccccgg cagggccatc gacctgatcg agcgcggtga
    15121 cctgtcggtc gcggacgtcc gccacgtggt catcgacgag gccgaccgca tggccgacat
    15181 ggggttcctg ccccaggtcg agtggatcct gcgcaacgtc gagggcgacc accagacgct
    15241 cctgttctcc gcaaccctcg acggggtggt cgacgggctg gtccggcgat accagcacga
    15301 cccgacccgc cacgaggtcg cctccaaggc ggtcaacgtc gagcagatga cccaccggtt
    15361 cctgctggtc cacgagatgg acaaggtgaa ggtcgcggcg tcgatcatcc gcaacgccca
    15421 gcgcaccatg gtgttctcgg ccaccaagca cggcgcggac cgcctggccc ggaagctcga
    15481 ggacgagggg gtcgacgcag caccgatcca cggggacctg cgacagaagt tccgggagaa
    15541 ggcgctgtcg gacttctccc acggcaggat ccgggcgctg gtcgccaccg acgtcgccgc
    15601 ccggggcatc cacgtcgacg acatcgacgt cgtcatccac tacgacccac cgagcgacca
    15661 caagacctac gtccaccgtt ccgggcgtac cgcccgcgcc ggcgaatccg gtgtggtcgt
    15721 gtcgctcgtg ctctacaacc aggagctcga gatcaagcgg ctccagaagc ggttgggcct
    15781 cgacatcccc atcgtcgaga tcttctccaa cgacccccgc ctggccgacg tggccgactg
    15841 gcccgaggac tgacgggccg gaacgccggc cgcaccagtg gtcgaccgcg accgcgtcga
    15901 gggctccggc ggtccgcgac accgctcggc gcgggacgcg gtggccgacc atgctcgggg
    15961 cctgaccgtt gatcggcggc gggagctggg atcccacgca acccccgaag cgctggcgct
    16021 ccatctggtc gagctggcgc tgcgacacct gcatcggctc ccgcagcgtg tgctcgaccc
    16081 ttcctgcgga gcggggtcct tcctgctcgc cgccgccgac gccctggtgc gccggggggc
    16141 ggatcccgcc gaggtggtgg agcagcggct cgagggctgg gacgtcgacc ccgaggcggt
    16201 ggcccactgc cgtgaggcgt tgcggcgctg ggccgcggcc cacggtgtgc gacggccggt
    16261 cgatgtccgc gtcgtcgagc tcgacgccct ggacccgacc gccggcccgg ccggctcggt
    16321 ggatctggtg gtcggcaacc cgccgttcct gtcccagcgc accctcgaca ctgcccggga
    16381 cgtcgcccgg cgtgagcagg tggatgcacg cttcggtccg ctggggccct acgtcgacga
    16441 ggcggccgtg ttcctcctcg tcgccgcgga gatgctctcg ccgggcggag tggccgtcat
    16501 ggtccagccg cgctcgaccc tgtcggcccg cgacgccggg gcggtccgcg atcgcctgct
    16561 ggaggtcgcc gcaccggtcg cggtgtgggc cgacgacggc cggcacttcg acgcggaggt
    16621 cgacgtgtgg gcgccggtgc tgcgtcgcgg cgtggacggc gaccgcggag aggaggtcga
    16681 acacggcgtc gaggtccact gggggaccgc ggcggacgcc gtggatcgtc cacggccaga
    16741 accgggccag agctggggac cgctcctcgc caccgcgctg ggcgtccccg agatcgcccc
    16801 ggcaggtgag atggcccccg ccggtgcggc ggggcatcgc gcgatcggcg acgtcgcgac
    16861 cgccaccgcc ggattccggg acgagttcta cgccctgagc gccgcggcac gatccctgga
    16921 cgagccgggg tgggggccgc agctcccccc tctggtcacc gtcggcatga tcgacgtggg
    16981 acgtctcgat cgacggcggc cccgccgcct cgggggtcgg ctcgtggccg acccccggct
    17041 cgacgtcgat tcgctccaga cggacgcacc ggcagtcgcg cgctgggccc ggaagcgaca
    17101 ggtgcccaag gtgctggtgg ccacccagac acgcgtgctc gaggcggtcg ccgacctcga
    17161 ccggcagatg gtccccgtca ccccgaccgt cagcgtggag ccgacggggg ccgtcggcgt
    17221 cggcccgcag gagctcctgg ccgcggtctg cgccccgccc tcggcggcgc gcctg
//
LOCUS       DAOQOD010000053        17132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1174_length_17132_cov_12.242724, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000053 DAOQOD010000000
VERSION     DAOQOD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17132
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_17132_cov_12.242724"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLZ72_07120"
     CDS             complement(<1..1306)
                     /locus_tag="PLZ72_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HNA36104.1"
                     /translation="MASLLDPLPIGSRTAPSRVVFGDHETNLGRGRVLSERHVAYYGR
                     RARGGAGVIVTEDASVHPSDWPLERAPLALECGPGWSAVVEACRPHGTVVLAGLGHAG
                     GQGTSHWSQRELWAPGRVPEVNTREVPKEMEADDIAAVVDGFADAARRAVVAGCDGVE
                     VNAGQFSLVRQFLSGLTNTRGDEWGEDRTLFARRVLAAVRDAVGADRVVGLRLSCDEL
                     APWAGITPEMATELAPALVLDDSGAPIVDLLTVVRGSIFSAGATRPDGHTAPGFNLEL
                     TRSIRDAVRAVAGDRVVVAAQGSIVDVEMAERAVADGMADAVEMTRAQLADADLVAKV
                     RAGQPERIRPCVLCNQTCQVRDNRNPIVTCVVDPTTGHETEDAPVPVEGAGVGGDGPA
                     VTVLGGGVAGLEAARVAALDGHRVTLHERGDRTGGSARIAAAL"
     gene            complement(1449..2648)
                     /gene="mftC"
                     /locus_tag="PLZ72_07125"
     CDS             complement(1449..2648)
                     /gene="mftC"
                     /locus_tag="PLZ72_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226361.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HNA36105.1"
                     /translation="MPLDRTVPPDVTVQPATATRSLVEEFKGGLDAPICLTWELTYAC
                     NLSCVHCLSSSGRRDPRELSTDECFAVVDELQRLQVFYVNIGGGEPMLRPDFFPIVQY
                     AVDHQVGVKFSTNGTFIDADAARRLAAMDYLDVQISIDGADAGVNDLIRGEGSYAAAR
                     RAMDNLADAGFGPFKISLVVTRHNVDQLDGFKALADSYGAQLRVTRLRPSGRGVDSWH
                     DLHPTQSQQRDLYHWLLGRNDVLTGDSFFHLSALGDPLPGLNLCGAGRVVCLIDPVGD
                     VFACPFVIHDEFRAGNVRDPGGFTRVWRESDLFTSLREPESPGACASCGSYDACQGGC
                     MASKFFVGLELTDPDPECVHGHAEPLLAAVGARGAAVPTPSADHSRPGVPVPSPVTLK
                     PRRRARV"
     gene            complement(2710..3027)
                     /gene="mftB"
                     /locus_tag="PLZ72_07130"
     CDS             complement(2710..3027)
                     /gene="mftB"
                     /locus_tag="PLZ72_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443073.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HNA36106.1"
                     /translation="MTAPVVTAPVGSVPVDPGDVLVLHPSVSLRPERFGALAYSFDTR
                     KLSFLKHPDLVRVVEALDGVHTVDDVLGSSGVDPSRWPSFRRALGTLVASQMLVAGGE
                     RAA"
     gene            complement(3047..3187)
                     /gene="mftA"
                     /locus_tag="PLZ72_07135"
     CDS             complement(3047..3187)
                     /gene="mftA"
                     /locus_tag="PLZ72_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HNA36107.1"
                     /translation="MPTDTQTTVEPAAESTDASVATDESVLVDDELLVEEVSIDGMCG
                     VY"
     gene            3419..3793
                     /locus_tag="PLZ72_07140"
     CDS             3419..3793
                     /locus_tag="PLZ72_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36108.1"
                     /translation="MTIDDAARLRLYDQARTNWGSDAADTLMSLMPTDPDSFATRDDM
                     AMLRSDLRGEMSELRGELRGEMSQLRNEMTELRGELRGEMTELRREMAVTSAETIRTI
                     VFAMLATNATLVGLVFAAVRLA"
     gene            3908..5035
                     /locus_tag="PLZ72_07145"
     CDS             3908..5035
                     /locus_tag="PLZ72_07145"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HNA36109.1"
                     /translation="MPTTTRAAVLWGHHEDWKIEEVELDDPGHGEVLVKTSYAGMCHS
                     DEHVVQGDMPVPHFPFIGGHEGSGVVEAVGPGVDTVAPGDHVAMSFVPSCGRCKWCAK
                     GQQNLCDLGAKLFDLQMITREPGSVSHRLGDTEAARFAQVGSFAEHMLLSQDSLVKIE
                     EDLPLDVAALVSCGVATGWGSAVYRANVTAGDTVAVIGVGGVGINAVQGAAMAGAKRV
                     IAIDPVEFKREKAMEMGATHTYSSIEEASLAVPDLTWGDMCDSVILVPGVVTGDMIEP
                     ALNLASKGGTLVVTAIGNMLETDTALNLFMLSMLNKEIKGTIFGSGNPRADIPNLLSM
                     YREGTLKLDELITNRYPLDDINRGYQDLRDGKNIRGVIDFT"
     gene            5032..6003
                     /locus_tag="PLZ72_07150"
     CDS             5032..6003
                     /locus_tag="PLZ72_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA36110.1"
                     /translation="MTDRSDPPSRPVLSGADLLRRLTTRVVGRRRELEAVVATLGADR
                     HLLLEGPPGTGKSTVLRAVAEESGVGFEFVEGNAELTPARLVGAFDPAAVLSEGYSPD
                     VFVDGPLTSALRDGSLLYVEEINRVPEETLNVLITVMSEGELHVPRLGHVVAAPGFRL
                     VAAMNPFDAVGTARIASAVYDRVCRLAMGYQGADEEAMIVAAAAPGAADAMGVDGVGR
                     AVAMVRDSRDHPDLRTGSSVRGAIDFVMVAGELAELRGLAVLDPGVTLDAALLSMSGR
                     IRLREGSGRSAEDVVTELWDGHFAQVDESDDGAAQPDGGDDGGSGKA"
     gene            5913..6959
                     /locus_tag="PLZ72_07155"
     CDS             5913..6959
                     /locus_tag="PLZ72_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA36111.1"
                     /translation="MGRPLRSGGRVRRRRGAARRRRRRRVGKSLTPPPGGGRPDSDDV
                     KEGDAAREAVADAARRTTSRRDLARHERFEQISPEVGVLDPDAFAEAMQDDADDALAL
                     LAELTGATDERLRDLARRLAGRVMVDLGRIGPERRRGVGRLRRVPRRDADGDIDLDSS
                     LDAVAAARAGVVTGDELRVAAWQRPSTAVCLLVDRSGSMAGDRLAAAAVAAAAVIFRA
                     PLDCSVVAFAEDAIVLKAQDEVRDPDEVVADLLRLRGFGVTDLGLALRTAAAQLARST
                     AGRRITLLMSDCRVTAGGDALVHAAGTHEVAVLAPNGDTADAEAFADALGARWTELAG
                     PTSVPDAISTVLLG"
     gene            complement(7061..8671)
                     /locus_tag="PLZ72_07160"
     CDS             complement(7061..8671)
                     /locus_tag="PLZ72_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA36112.1"
                     /translation="MTEATTIDAARAAAFQFEGQDIAWLVDHWATHRPDKVFLIWEPK
                     DGPSRSWTYAEFAEETKALAAGLAARGVGVGDMVLIHAENCPEGVIGWYACARVGAVA
                     VTTNTRSVAAEVNYFITHTGCVGAITQPRYAALVAEAGPDLGWIVVTDDNSGVAATDE
                     EAAHGRDGWASLHGDPSDAPERDRDPMLPAGILFTSGTTSKPKAVVHTHANALWAAKV
                     NPTNIQLEADDVYLASLPFFHVNTQSWAIWSILGVGGTVVLQPKFSASGFWPVIEKHS
                     VTHISLLPFVIQNAFEQGIPEDHTVKVGVFGLIMPVLDQMMGMKVVAAYGMTELVTHC
                     IHSNPWEHYPDMAMGKVSPGYEMLVVTEDGSLAEVGQMGELWIRGIRGVSVCLEYFHN
                     DEANAKFFTDDGWARTGDIVMLLEDGNIRYCDRDKDALKVGGENVSAREVEDVIRTVP
                     GLTDAQVVAKSHPRLDMVAVAFVIKADPAADEAELSAQIIDACRQNLADFKVPRAVYF
                     VMEYPTAELGKISKKDLRELADSFDPVD"
     gene            complement(8822..10852)
                     /locus_tag="PLZ72_07165"
     CDS             complement(8822..10852)
                     /locus_tag="PLZ72_07165"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNA36113.1"
                     /translation="MTSDAADPGSDALWYPPADRVRCSRMRSFITTAAAHGGGELADT
                     AALHRWSVERPEEFWRLALGELLPAVDDSGPVWERGAHMADVRWFPEVSMNVAEQILA
                     AGPPDGLLAVAVDERGRRRELTRAEATAEVGRVAAALRAAGVGVGDRVVAWLPNTIET
                     VLLMLGAASIGAVFSSSSPDFGADGVLDRFAQITPTVLVAADGYTYGGRAFDRTPELA
                     TVIEGLPTLRQVVVVPFLRDDPDLSGVPGSPATWDDWLEPFRGAVVEFVPLPFDHPWY
                     VLFSSGTTGVPKCIVHRSGGVLLTHAKEQQLHLDVGPGDRVLYFTTCGWMMWNWLASV
                     PASGAAIVLYDGNPAHPGPERLWELAAAEGVHLLGVSAKFIDAVRHSGYRPGERTDLA
                     SLRTLASTGSPLSPEGFEWVMDAVAPGVGPDGLHLASISGGTDLCGCFVLGDPTRPVH
                     AGEIQGPALGMAVDVWTDAGTPAAVGERGELVCTGPFPSMPLGFWGDEPDGGPPGGVD
                     DHLGPRYRAAYFERFPGVWAHGDFATWTVHGGMVIHGRSDTTLNPGGVRIGTSEIYRQ
                     VERVEGVEESLVFGQQTDEDVRIVLLVRLAAGTALTDDLEAQIRARVRTGCTPRHVPA
                     VIVAVADLPRTRSGKLAELAVADTVNGRPVRNTTALANPEVLADIAAAVPAP"
     gene            10935..12416
                     /locus_tag="PLZ72_07170"
     CDS             10935..12416
                     /locus_tag="PLZ72_07170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA36114.1"
                     /translation="MERLSGMDATFLYLETPAGHMHVAMTGIYDASTMPGGYSFETIK
                     QTIGERVPLVPPFRRRLVEVPFNLHHPVWIEDPDFDLDAHVHRVVCPAPGGRRELARL
                     AGQIASTPIDRTRPLWEAWVIEGLKHDRFGFIAKVHHATIDGSGGAELMTALFDLDPS
                     GREVDVEPVAVEHVPSDLELLSYAALSKAKRLGDAAGLIGRTAQSVTNVVTGIRNPER
                     RHGAVPLTAPRTPMNASITPDRSVAFARVSLDEVKAVKEALGVKVNDVILAICSGTLR
                     HYLKGIDALPEEPLLAVCPVSVRTEDERHSQGNKVSAMFTSLATDVEDPFERIQAIAN
                     STAGAKEDHNAVGARTLTDWAEWATPRTFGLAARLYSSMNLANSHRPIHNLVISNVPG
                     PPFPLYMSGAEMVATYPMGPIMDGAGLNITVMSYRENVDIGYLGCTDLVPDIWDIADA
                     TSVAFDEIKQAVAELDPTITKAPAPVTTTRTSTSNGRRSATDQ"
     gene            12446..14071
                     /locus_tag="PLZ72_07175"
     CDS             12446..14071
                     /locus_tag="PLZ72_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA36115.1"
                     /translation="MAYNIADLFEHTVDAVPNRTALICGDTRLTFAELDERANRFGHF
                     LQSRGVRPGDHVGIYAVNSEPWVTAMLGCVKIRAVPVNVNYRYVEAELAYLIGNADLV
                     ACVFDQEYAPRLAAVVADAPKLRTLVHIADGSGADTSALGSTDFDDACAEGSPERDFE
                     DRSDDDVYIIYTGGTTGMPKGVMWRHEDIYFALGQGIDALTNERVASEFTKAEQAAAS
                     ETPLIFGVIPPLMHGAAQIATMSQWFIGSTIVLVSKFDPAGVWDLVDAHGVNSVIITG
                     DAVARPMIEALEDEPDRWDASSLISVSSSAALFSQSVKERFLDRFPNLIITDSIGSTE
                     GGFNGVTYAAKGATVDGGGPTVNPGRDVAVLDDDLQVIPPGDTRVGRLGRTGNIPLGY
                     YNDPAKTAEVFVTAGDGRRYSMGGDFARWTEDGRLVMLGRGSVSINSGGEKIFPEEVE
                     AALKSHPAVYDCLVVGVPDERWGQRVAAIVELRDGHDVTLDELAGHARTLVAGYKIPR
                     ELHVVDQIVRSPSGKPDYPWAKELATRGDARVG"
     gene            14061..15635
                     /locus_tag="PLZ72_07180"
     CDS             14061..15635
                     /locus_tag="PLZ72_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNA36116.1"
                     /translation="MSADAGPDDTSSGCGPRNVVVILLDSLNRHMVGAYAPVLAQAPE
                     FDTPNLDRFAARAVRFDRHHTGSLPCMPARHDILVGALDFLWRPWGSVEIWEDAVTYD
                     LRRAGVTTMLVSDHPHLFEAGGENYHTDFGAWRYVRGHEGDPWRTAADPSWVGTPALP
                     AREGWIPHAYDESRTWFRSEEDFPGARTMAEAARWLADEAPSAERFMLFVDEFDPHEP
                     FDVPEPWMSRYDPDWDGPRVIWPPYAVDAVASGVIDERTARHIRANYGAKLSFVDHQF
                     GRLLDELDRRDLWDDTAVIVCTDHGHYLGEHDIFGKPSTPVRAVLGHIPLMVAWPGRG
                     PGVVDRLTTTVDIHATICDVFGVTPEHRTHGRSLVPLLDDPAGGPAREWALTGIWGQG
                     VTVTDGRRRYLRAPDPGNRPLSMWSNRWSTMPIHAFPELRLPRPDGRATLERMPGSDV
                     PVIHQPFGPGDPVPYWAAAEGHSQTSLLFDLDDDPAELHDLTGTATEVEMVDLLRAAL
                     GEIDAPGDQLVRLGLS"
     gene            complement(15646..16230)
                     /locus_tag="PLZ72_07185"
     CDS             complement(15646..16230)
                     /locus_tag="PLZ72_07185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA36117.1"
                     /translation="MRWTVHGERPIYESEWVRLVLTDVEVPAVPGHPARRFEHHVVRS
                     RGPAAGAVVLDDVETDDAGPRPCVLLLWRHRFITDTWGWEVPGGGVDPGEASIDAAAR
                     EVLEESGWRIGGDAGTVEHLVDYFPMNGLGDNRFDLFLARGAVHEGDPTDPSEAERVE
                     WVPVDRLRGEISAGRVQDGLSLSALLWCLAFGLL"
     gene            complement(16261..16917)
                     /locus_tag="PLZ72_07190"
     CDS             complement(16261..16917)
                     /locus_tag="PLZ72_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNA36118.1"
                     /translation="MTGFVRDVALGVATGLPMLVVVGPIALLLVDVGVRRGLRAGSPA
                     ALGVALGDVVLAVVVAVAGTALGSRLAPVADQLRIGAAVLVTAIAVHAVLSVRRAGAS
                     GPGGSERSVPVLAPAGGPGESSSDHGTGRLLGGFAALTLANPLTLLVYTGVVVGGGAG
                     VGTAGWVTGMGLASLAVHHGWIGVGHVVGSALPSGALRVARYVAAGLLLGLAVHLLVG
                     "
BASE COUNT         2506 a         6252 c         6087 g         2287 t
ORIGIN      
        1 ggagagcggc cgcgatccgg gccgacccgc cggtgcggtc gccccgctcg tgcagagtca
       61 cccggtggcc gtcgagcgcc gccacccggg cggcctccag acccgcgact ccgccgccga
      121 ggacggtcac cgccgggccg tccccgccga cgccggcgcc ttcgacgggc acgggagcgt
      181 cttcggtctc gtgtccggtc gtcggatcga cgacgcaggt cacgatcggg ttgcggttgt
      241 cgcggacctg gcaggtctgg ttgcacagca cgcagggtcg gatccgctcc ggctgcccgg
      301 cccggacctt ggccaccagg tcggcgtcgg ccagctgcgc ccgggtcatc tcgaccgcgt
      361 cggccatgcc gtcggccacg gcgcgctcgg ccatctcgac gtcgacgatc gagccctgcg
      421 cagccaccac cacgcggtcc ccggccaccg cccgcaccgc gtcacggatc gagcgggtca
      481 gctccaggtt gaaccccggc gcggtgtggc cgtcggggcg ggtggcgccc gcggagaaga
      541 tcgagccgcg cacgacggtg agcaggtcga cgatcggggc gccggagtcg tcgagcacga
      601 gtgcgggggc gagctcggtg gccatctcgg gggtgatgcc ggcccagggg gcgagctcgt
      661 cgcagctcag acggagcccg acgacccggt cggcaccgac cgcgtcgcgc accgcggcca
      721 gcacccggcg ggcgaacagc gtccggtcct cgccccactc gtcgccgcgg gtgttggtca
      781 accccgacag gaactggcgc accagggaga actggccggc gttgacctcg acgccgtcgc
      841 agcccgcaac gactgcccgt cgcgccgcgt cggcgaaccc gtcgacgacc gccgcgatgt
      901 cgtcggcctc catctccttg gggacctcgc gggtgttcac ctcggggacc cgacccggcg
      961 cccacagctc gcgctgggac cagtgcgagg tgccctggcc cccggcgtgg ccgagcccgg
     1021 ccagcacgac cgtcccgtgg ggtcggcacg cctcgaccac cgccgaccag ccggggccgc
     1081 actcgagcgc gagcggagcg cgttcgagcg gccagtccga cgggtgcacc gaggcgtcct
     1141 cggtgacgat cacgccggcg ccgccgcggg cccgacgccc gtagtaggcg acgtggcgct
     1201 ccgagagcac ccgaccgcgt ccgaggttgg tctcgtggtc gccgaacacg acgcggctcg
     1261 gcgcggtccg ggagccgatc ggcaacgggt cgagcaggga cgccatcggc cgccatggtg
     1321 ccccaccggc gccgccgggg ccgaacccac acccgcccca gccacatata ggggtgctca
     1381 acgacacacc ttgtcgcaat ccgcgcccca gaaaccggca gccgcggcgg ccggaggtga
     1441 tcagctgatc agacgcgggc gcggcgcctg ggcttcagcg tgaccggcga cgggacgggc
     1501 acacccggcc gcgagtggtc ggccgacggg gtcggcaccg cggctccccg ggcgccgacc
     1561 gcggcgagca gcggctcggc gtggccgtgg acgcactcgg ggtccgggtc ggtcagctcg
     1621 agcccgacga agaacttgga ggccatgcag cccccctggc atgcgtcgta gctgccgcag
     1681 ctggcgcacg cccccgggct ctccggttcg cggagcgacg tgaagagatc cgactcccgc
     1741 cagacccggg tgaacccgcc ggggtcgcgg acgttgccgg cccggaactc gtcgtggatg
     1801 acgaacgggc aggcgaacac gtcgccgacc gggtcgatca ggcagacgac ccgaccggcg
     1861 ccgcacaggt tgagcccggg cagcggatcg ccgagggcgg acaggtggaa gaacgagtcg
     1921 ccggtgagca cgtcgttgcg accgagcaac cagtggtaca ggtcccgctg ctgggactgc
     1981 gtggggtgca ggtcgtgcca ggagtccaca cctctgcccg aggggcgcag ccgggtcacg
     2041 cgcagctgcg cgccgtacga gtcggccagg gccttgaacc cgtcgagctg atcgacgttg
     2101 tggcgggtga cgaccaggct gatcttgaac ggcccgaacc cggcgtcggc caggttgtcc
     2161 atcgcacggc gggctgccgc gtaggaaccc tcaccgcgga tcaggtcgtt gaccccggcg
     2221 tcggcgccgt cgatggagat ctgcacgtcg aggtagtcca tggcggccag gcgtcgtgcc
     2281 gcgtcggcat cgatgaaggt gccgttggtg gagaacttga ccccgacctg gtggtcgacg
     2341 gcgtactgca cgatcgggaa gaagtccggg cgcagcatcg gttcgccgcc gccgatgttg
     2401 acgtagaaca cctggagtcg ctgcagctcg tcgacgaccg cgaagcactc gtcggtgctg
     2461 agctcccggg ggtcacgccg acccgagctc gacaggcagt gcacgcagct gaggttgcag
     2521 gcgtaggtca gctcccaggt gaggcagatc ggggcgtcga gccctccctt gaactcctcg
     2581 accagcgaac gggtcgccgt ggccggctgg acggtgacgt cgggcggcac ggtcctgtca
     2641 agcggcacgg tcctgtcaag cggcacggtc ctgtcaggca gcacggtcct gtcaggcagc
     2701 acggtcctgt caggcggcac gttcgcctcc ggccacgagc atctgcgacg cgacgagcgt
     2761 gcccaacgcc cggcggaagc tcggccaccg cgacgggtcc acgccggaac tcccgagcac
     2821 gtcgtcgacc gtgtgcaccc cgtcgagggc ttcgacgacg cgcaccaggt cggggtgctt
     2881 gaggaacgag agcttgcggg tgtcgaagct gtaggcgagc gcgccgaagc gctcgggcct
     2941 cagggacacc gaggggtgca gcacgagcac gtcaccggga tcgacgggaa cggacccgac
     3001 aggagcggtc acgacaggag cggtcacgac aggaccggaa cccgactcag tagacgccgc
     3061 acatgccgtc gatcgagacc tcttcgacca gcagttcgtc gtccaccagg acggactcgt
     3121 cggtggccac ggacgcatcg gtcgattcgg cggcgggctc gacggtggtc tgggtgtcgg
     3181 tcggcattcg ttgctcctcc gggaggtcga tggcggaatc gtaccggccc cggcccgctc
     3241 gacccacgcg agaccacggg ccctcggccc tccgggaccg gaacccgtcg tcacacccgt
     3301 cgaggaggat cccgaccgga acctcccgga atcggtgccc tcggggcgcc ctcttcgaca
     3361 cgcctgtcga cgatcaggac gagggcaccc gttgacggtg ccggagagcg agaccaccat
     3421 gaccatcgac gacgcggcgc ggctgcgcct gtacgaccag gctcggacca actggggctc
     3481 cgacgccgcc gacaccctga tgagcctcat gcccaccgac cccgactcct tcgccacccg
     3541 cgacgacatg gcgatgctgc gaagcgacct gcgcggcgag atgagcgagc tgcgcggcga
     3601 gctccggggc gagatgagcc agctccggaa cgagatgacc gagctgcgcg gcgagctccg
     3661 gggcgagatg accgagctgc gccgggagat ggccgtcacc tcggccgaga cgatccgcac
     3721 gatcgtgttc gccatgctgg ccaccaacgc caccctcgtc ggcctggtgt tcgccgcggt
     3781 ccgcctggcc tgacggcggg ccaatgtgac tccggtcggc ccggatcctg tcgaccaccg
     3841 gtggggatag agtcgccgtc gatcgaacgc ggaccgcccg gcccgccgca gaacaggggg
     3901 acaaccgatg ccgaccacca cccgcgccgc cgtcctgtgg ggccaccacg aggactggaa
     3961 gatcgaagag gtcgagctcg acgacccggg ccacggcgag gtcctcgtca agacctccta
     4021 cgccggcatg tgccactccg acgagcacgt ggtgcagggc gacatgccgg tcccgcactt
     4081 cccgttcatc ggcggtcacg aggggtccgg agtggtcgag gcggtcggcc ccggcgtgga
     4141 caccgtcgcc cccggcgacc acgtcgccat gtccttcgtg cccagctgcg gtcgctgcaa
     4201 gtggtgtgcc aagggccagc agaacctgtg cgacctgggc gccaagctgt tcgacctgca
     4261 gatgatcacc cgagagcccg gcagcgtctc gcaccgcctc ggcgacaccg aggccgcccg
     4321 cttcgcccag gtcggttcgt tcgccgagca catgctgctc agccaggact cgctggtgaa
     4381 gatcgaggag gacctccccc tcgacgtcgc cgcgctcgtc tcctgcggcg tcgccaccgg
     4441 gtggggctcc gcggtctacc gggcgaacgt gaccgcgggc gacaccgtcg cggtcatcgg
     4501 cgtgggcggc gtcggcatca acgccgtgca gggcgcggcc atggccggcg ccaagcgcgt
     4561 gatcgccatc gaccccgtcg agttcaagcg cgagaaggcc atggagatgg gggccaccca
     4621 cacgtactcg tcgatcgaag aggcgtcgct cgcggtcccg gatctgacct ggggcgacat
     4681 gtgcgactcg gtgatcctgg tccccggcgt ggtgaccggc gacatgatcg aaccggcgct
     4741 caacctggcc tccaagggag gcaccctggt ggtcaccgcg atcggcaaca tgctcgagac
     4801 cgacacggcg ctgaacctgt tcatgctctc gatgctgaac aaggagatca agggcacgat
     4861 cttcggttcg ggcaaccccc gcgccgacat ccccaacctg ctgtcgatgt accgcgaggg
     4921 caccctcaag ctcgacgagc tgatcaccaa ccggtacccg ctcgacgaca tcaaccgggg
     4981 ctaccaggat ctgcgcgacg gcaagaacat ccgcggggtc atcgacttca cgtgaccgac
     5041 cgctcggacc cgccgtcccg cccggtcctc tcgggcgcgg acctgctgcg ccgcctcacg
     5101 acccgggtcg tggggcggcg acgcgagctg gaggccgtcg tcgcgaccct cggagcggat
     5161 cgccacctgt tgctcgaggg gccgccgggc accggcaagt cgacggtcct gcgggcggtg
     5221 gccgaggagt ccggcgtcgg cttcgagttc gtcgagggca acgccgagct gaccccggcc
     5281 cgcctcgtcg gcgcgttcga ccccgccgcg gtgctctcgg agggctactc ccccgacgtg
     5341 ttcgtagacg gcccgctgac ctcggcgctg cgcgacggct ccctcctgta cgtcgaggag
     5401 atcaaccgcg tccccgaaga gaccctgaac gtcctcatca ccgtgatgag cgaaggcgag
     5461 ctgcacgtcc cccggctcgg ccacgtcgtc gcggccccgg ggttccggct ggtggcggcc
     5521 atgaacccgt tcgacgcggt gggcaccgcc cgcatcgcgt ccgcggtgta cgaccgggtg
     5581 tgccgcctgg cgatgggcta ccagggggcc gacgaggagg cgatgatcgt cgccgccgcc
     5641 gcccccggcg ctgccgacgc gatgggcgtc gacggcgtgg gccgggccgt cgccatggtg
     5701 cgcgacagcc gcgaccaccc ggacctgcgc accggctcgt cggtccgcgg tgcgatcgac
     5761 ttcgtgatgg tggccggcga gctcgccgag ctccggggtc tggccgtgct cgaccccggg
     5821 gtgaccctcg acgcggcgct gctctccatg tcgggtcgga tccggctccg ggaggggtcc
     5881 ggccgcagcg ccgaggatgt cgtcaccgag ctgtgggacg gccacttcgc tcaggtggac
     5941 gagtccgacg acggcgcggc gcagcccgac ggcggagacg acggcgggtc gggaaaagcc
     6001 tgaccccgcc acccggtggg ggccgcccgg acagcgacga cgtcaaggag ggtgacgcgg
     6061 cgcgcgaggc ggtcgccgac gccgcccggc gcaccacctc gcgtcgggac ctggcccgcc
     6121 acgagcgctt cgagcagatc tcacccgagg tcggcgtcct cgaccccgac gcgttcgccg
     6181 aggcgatgca ggacgacgcg gacgacgccc tggccctgct cgcggagctg accggggcga
     6241 ccgacgagcg actccgggac ctggcccgcc ggctggccgg gagggtgatg gtcgacctgg
     6301 gtcggatcgg cccggagcga cggcgcgggg tggggcggct gcggcgggtt ccccgccggg
     6361 acgcggacgg cgacatcgac ctggacagct cgctcgacgc cgtcgccgcc gcccgggccg
     6421 gggtcgtcac cggcgacgag ctgcgcgtcg cggcgtggca gcgaccgtcg accgcggtgt
     6481 gcctgctggt cgaccggtcc gggtccatgg ccggcgaccg gttggccgcg gcggccgtcg
     6541 cggccgcggc ggtgatcttc cgggcgccgc tcgactgctc ggtggtggcc ttcgccgagg
     6601 acgccatcgt gctcaaggcc caggacgagg ttcgcgaccc cgacgaggtg gtcgccgacc
     6661 tgctgcgact ccggggcttc ggggtgacgg acctcggtct ggctctgcgg accgcggcag
     6721 cgcagctggc ccgatcgacc gcggggcgtc gcatcacgtt gctcatgtcg gactgtcggg
     6781 tgaccgccgg cggtgacgcc ctggtgcacg cggccgggac ccacgaggtg gcggtgctcg
     6841 cgccgaacgg cgacaccgcg gacgccgagg cgttcgccga cgcgttgggg gcccgctgga
     6901 ccgagctggc cggcccgacg tcggtccccg acgcgatcag caccgtcctg ctcggctgac
     6961 aggcgctgct cggctgagcg gcgctgccct cccccgcgtt ctcatgaccg ttcgcgccac
     7021 cggtggcgcg gtcggtcacg agaacgcggg atcgtgctgt tcagtcgacc gggtcgaacg
     7081 agtcggccag ctcgcgcagg tccttcttgg agatcttgcc gagctcggcg gtggggtact
     7141 ccatcacgaa gtagaccgcg cgcgggacct tgaagtcggc gaggttctgt cggcacgcgt
     7201 cgatgatctg cgccgacagc tccgcctcgt cggccgcagg gtcggccttg atcacgaacg
     7261 cgacggcgac catgtcgagg cgtggatgcg acttggcgac gacctgtgcg tcggtcagac
     7321 cgggaaccgt gcggatcacg tcctcgacct cgcgggccga cacgttctcg ccgccgacct
     7381 tgagggcgtc cttgtcgcgg tcgcagtagc ggatgttgcc gtcctcgagc agcatcacga
     7441 tgtcgccggt gcgggcccag ccgtcgtcgg tgaagaactt cgcgttggcc tcgtcgttgt
     7501 ggaagtactc gaggcagacc gagacgcccc ggatgccccg gatccacagc tcgcccatct
     7561 ggccgacttc ggcgagggag ccgtcctcgg tgacgacgag catctcgtag cccggcgaca
     7621 ccttccccat cgccatgtcg gggtagtgct cccacgggtt ggagtggatg cagtgggtga
     7681 ccagctcggt catcccgtag gcggcgacga ccttcatgcc catcatctgg tcgaggaccg
     7741 gcatgatcag gccgaacacc ccgaccttga cggtgtggtc ctcggggatc ccctgctcga
     7801 acgcgttctg gatgacgaac ggcaggagcg agatgtgggt caccgagtgc ttctcgatga
     7861 ccggccagaa gccgctggcg gagaacttgg gctgcagcac gacggtgccg ccgacgccga
     7921 ggatcgacca gatcgcccag ctctgggtgt tgacgtggaa gaacggcagc gacgccaggt
     7981 acacgtcgtc ggcctcgagc tggatgttgg tcgggttgac cttggcggcc cacagcgcgt
     8041 tggcgtgggt gtgcaccacg gccttgggct tggaggtggt gcccgaggtg aacaggatcc
     8101 cggccggcag catcgggtcg cggtctcgtt ccggggcgtc ggagggatcg ccgtgcagcg
     8161 acgcccagcc gtcacggccg tgggcggcct cctcgtcggt cgcggcgact ccggagttgt
     8221 cgtcggtgac gacgatccag ccgaggtccg ggcccgcctc ggccaccagc gccgcatatc
     8281 gcggctgggt gatggccccg acgcagccgg tgtgggtgat gaagtagttg acctccgcgg
     8341 cgacgctgcg ggtgttggtg gtcaccgcca cggccccgac ccgtgcgcac gcgtaccagc
     8401 cgatgacccc ctcggggcag ttctcggcgt ggatgagcac catgtcgccg acgccgacgc
     8461 cccgggcggc gaggccggcg gcgagcgcct tcgtctcctc ggcgaactcg gcgtaggtcc
     8521 agctccggga cggtccgtcc ttgggctccc agatcaggaa gaccttgtcg ggtcggtggg
     8581 tggcccagtg gtcgacgagc caggcgatgt cctggccctc gaactggaac gccgcggccc
     8641 gcgccgcgtc gatggtggtg gcttcggtca tctgggacgc cccctgtgcg ttctcggtcc
     8701 cgtgctctgg cggacctgac gatatgtcag ttcccgcgga gccgacgccg acccggggtt
     8761 cgcgtgtgag aacgactcct ggcacgtcga tcccacacgc gaacaccgcg cgggccctgg
     8821 gtcagggggc cggcaccgcg gcagcgatgt cggccagcac ctcggggttg gccagcgcgg
     8881 tggtgttgcg gaccgggcgc ccgttgacgg tgtcggccac ggccagctcg gcgagcttgc
     8941 ccgaacgggt ccggggcagg tccgccacgg cgacgatcac cgccgggacg tgacggggcg
     9001 tgcagccggt gcggacccgt gcgcggatct gcgcctccag gtcgtcggtc agcgcggtcc
     9061 cggcggccag cctcaccagc agcacgatgc ggacgtcctc gtcggtctgc tggccgaaca
     9121 ccaacgactc ctcgacgccc tcgacacgct cgacctggcg gtagatctcg gaggtgccga
     9181 tgcgcacgcc cccggggttg agcgtcgtgt cggaacgccc gtggatgacc atccctccgt
     9241 ggacggtcca ggtggcgaag tcgccgtggg cccacacgcc ggggaaccgc tcgaagtacg
     9301 cggcgcggta gcgggggccc aggtggtcgt cgaccccacc gggagggccg ccgtcgggct
     9361 cgtcgcccca gaagcccagg ggcatcgacg ggaacggtcc ggtgcacacg agctcgccgc
     9421 gctcgccgac cgcggccggc gttccggcat ctgtccacac gtcgacggcc atccccagcg
     9481 ccggaccctg gatctcaccg gcgtggacgg ggcgggtcgg gtccccgagc acgaagcagc
     9541 cgcacaggtc ggtccctccc gagatggagg ccaggtgcag gccgtcggga ccgaccccgg
     9601 gggcgaccgc gtccatcacc cactcgaagc cctccggcga cagcggcgaa cccgttgagg
     9661 cgagcgtccg caacgaggcg aggtcggtcc gctcccccgg gcggtatccg gagtggcgca
     9721 ccgcgtcgat gaacttggcc gacacgccga gcaggtggac accttctgcg gccgccagct
     9781 cccacagccg ctcggggccg gggtgggcgg ggttgccgtc gtacaggacg atcgccgccc
     9841 ccgacgccgg caccgacgcc agccagttcc acatcatcca gccgcacgtc gtgaagtaca
     9901 ggacccggtc gccgggcccg acgtcgaggt gcagctgctg ctccttggcg tgggtgagca
     9961 gcaccccgcc gctgcggtgc acgatgcact tgggcacccc ggtggtcccg gagctgaaca
    10021 gcacgtacca cgggtggtcg aacggcagcg gcacgaactc gaccacggcg ccccggaacg
    10081 gttcgagcca gtcgtcccag gtggcgggag accccgggac ccccgacagg tcggggtcgt
    10141 cgcggaggaa cggaaccacc acgacctgcc ggagcgtcgg cagcccctcg atgaccgtgg
    10201 ccagctccgg ggtccggtcg aaggcgcggc cgccgtaggt gtagccgtcg gcagcgacca
    10261 ggaccgtcgg ggtgatctgg gcgaaccggt cgagcacccc gtcggctccg aagtccggcg
    10321 acgacgacga gaacaccgcc ccgatcgacg cggcgccgag catgagcagg accgtctcga
    10381 tcgtgttggg cagccaggcg acgacccggt cgccgacgcc gacgccggcg gcccgcaggg
    10441 cggcggcgac ccggccgacc tcggccgtgg cctcggcccg ggtcagctcg cgtcggcggc
    10501 cccgctcgtc gaccgcgacc gccagcagcc cgtcgggtgg tccggcggcc aggatctgct
    10561 cggcgacgtt catgctgacc tcggggaacc aacggacgtc ggccatgtgg gcgccgcgct
    10621 cccacaccgg ccccgagtcg tccaccgccg ggagcagctc gcccagggcg aggcgccaga
    10681 actcctcggg tcgctcgacc gaccaacggt ggagggcggc cgtgtcggcc agctcccctc
    10741 cgccgtgcgc cgcggccgtc gtgatgaagg accgcatccg ggagcaccgc acccggtccg
    10801 ccggcgggta ccagagggcg tcggaaccgg ggtcggcggc gtcggaggtc atcgactcgg
    10861 cctaccacag cagcggggcg cgatgatggt gtccccgccg cggccgcggc ggctcgtcat
    10921 caggagggac ccaggtggaa cgactgtcgg gaatggacgc cacgttcctg tacctggaga
    10981 cgccggccgg gcacatgcac gtggccatga ccggcatcta cgacgcctcg acgatgcccg
    11041 gcggctacag cttcgagacg atcaagcaga cgatcggcga gcgggtcccc ctggtccccc
    11101 cgttccggcg gcgcctggtc gaggtcccct tcaacctcca ccacccggtg tggatcgagg
    11161 accccgactt cgacctcgac gcccacgtgc accgcgtggt gtgcccggcg cccggcgggc
    11221 gccgtgagct ggcccgactg gccgggcaga tcgcctcgac gccgatcgac cgcacccgtc
    11281 ccctgtggga ggcgtgggtg atcgagggcc tcaagcacga ccgcttcggt ttcatcgcca
    11341 aggtccacca cgccacgatc gacggctccg gcggcgcgga gctgatgacc gccctgttcg
    11401 acctcgaccc ctccggccgc gaggtcgacg tggagccggt cgcggtcgag cacgtgccca
    11461 gcgacctgga gctgctgtcg tacgcggcgc tgtccaaggc caagcggttg ggcgatgccg
    11521 ccggcctgat cggccgcacc gcccagtcgg tgaccaacgt cgtcaccggc atccgcaacc
    11581 ccgagcgccg tcacggcgcc gtcccgctca ccgcgccgcg caccccgatg aacgcgtcga
    11641 tcacccccga ccgctcggtc gcgttcgccc gcgtctccct cgacgaggtc aaggcggtca
    11701 aggaggcgct gggcgtcaag gtcaacgacg tgatcctggc catctgctcg ggcacgctgc
    11761 gccactacct gaagggcatc gacgccctgc ccgaggagcc gctgctggcg gtgtgcccgg
    11821 tgtcggtgcg caccgaggac gaacgccact cccagggcaa caaggtgtcg gccatgttca
    11881 cctcgctcgc caccgacgtc gaggacccgt tcgagcggat ccaggccatc gccaactcca
    11941 cggccggagc caaggaggac cacaacgcgg tcggggcgcg gaccctcacc gactgggccg
    12001 agtgggccac ccctcgcacc ttcggcctgg ccgcccggct gtacagctcg atgaacctgg
    12061 ccaactcgca ccggccgatc cacaacctgg tcatctccaa cgtccccggt ccgccgttcc
    12121 cgctgtacat gtcgggcgcg gagatggtgg cgacctaccc gatgggcccg atcatggacg
    12181 gagccggact caacatcacg gtgatgagct accgcgagaa cgtcgacatc ggctacctcg
    12241 gctgcaccga cctcgtcccc gacatctggg acatcgcgga cgccacctcg gtggccttcg
    12301 acgagatcaa gcaggccgtc gccgagctcg acccgaccat caccaaggcc ccggcgccgg
    12361 tcaccaccac ccggacctcg acgtcgaacg ggcgccgcag cgcgacagat cagtgagcac
    12421 cgcagcgact cggtagcgtg ccgccgtggc ctacaacatc gctgacctgt tcgagcacac
    12481 cgtcgacgcg gtgccgaatc gcaccgcact catctgcggc gacacgcggc tgaccttcgc
    12541 cgagctcgac gagcgggcca accgcttcgg ccacttcctg cagtcccggg gtgtccggcc
    12601 cggagaccac gtcggcatct acgcggtcaa ctccgagccg tgggtgaccg ccatgctcgg
    12661 ctgcgtcaag atccgtgccg tcccggtcaa cgtcaactac cgctacgtcg aagccgagct
    12721 ggcctacctg atcggcaacg ccgatctggt ggcctgcgtc ttcgaccagg agtacgcccc
    12781 gcggctggcc gcggtcgtcg cggacgcgcc caagctccgc acgctggtgc acatcgccga
    12841 cggctccgga gcggacacgt ccgcgctcgg gtcgaccgac ttcgacgacg cgtgtgccga
    12901 gggatcgccc gagcgggact tcgaggaccg ctccgacgac gacgtctaca tcatctacac
    12961 cggcggcacg accggcatgc ccaagggcgt gatgtggcgc cacgaggaca tctacttcgc
    13021 tctcggccag gggatcgacg ccctgacgaa cgagcgggtc gccagcgagt tcaccaaggc
    13081 ggagcaggcc gcggcgtcgg agactccgct gatcttcggg gtgatcccgc cgctcatgca
    13141 cggcgcggcg cagatcgcca ccatgagcca gtggttcatc ggctcgacga tcgtgctggt
    13201 gtccaagttc gaccccgccg gggtgtggga cctggtcgac gcccacggcg tcaactcggt
    13261 gatcatcacc ggcgacgcgg tggcccggcc gatgatcgag gcgctcgagg acgagccgga
    13321 ccgctgggac gcgtccagcc tgatctcggt gtcgtcgagc gcggcgctgt tctcgcagtc
    13381 ggtcaaggag cggttcctcg accggttccc gaacctgatc atcaccgact cgatcggctc
    13441 caccgagggc ggcttcaacg gcgtcaccta cgcggccaag ggcgcgacgg tcgacggcgg
    13501 tggcccgacg gtgaacccgg gtcgcgatgt cgcggtgctc gacgacgacc tgcaggtgat
    13561 cccgccgggc gacacgcggg tcggtcgact gggccgcacc ggcaacatcc cgctcggcta
    13621 ctacaacgac ccggccaaga ccgccgaggt gttcgtgacc gccggcgacg gacggcgcta
    13681 ctcgatgggc ggcgacttcg cccgctggac cgaggacggc cggttggtga tgctcggccg
    13741 gggttcggtg tcgatcaact ccggcggcga gaagatcttc cccgaggagg tcgaggccgc
    13801 cctcaagagc caccccgcgg tgtacgactg cctggtggtc ggcgtgcccg acgagcggtg
    13861 ggggcagcgg gtggcggcga tcgtcgagct gcgtgacggc cacgacgtca ccctcgacga
    13921 gctggccggg cacgcccgga ccctggtggc cggctacaag atcccccgcg agctgcacgt
    13981 ggtcgaccag atcgtccgct ccccctccgg caagcccgac tacccgtggg ccaaggagct
    14041 cgccacccgg ggcgacgccc gtgtcggctg acgcaggtcc cgacgacacc tcctcgggtt
    14101 gcgggccccg caacgtggtc gtcatcctgc tcgacagcct caaccgccac atggtcggcg
    14161 cctacgcccc ggtcctggcc caggcccccg agttcgacac gccgaacctc gaccgcttcg
    14221 cggcccgggc ggtgcgcttc gaccgccacc acaccggatc gctgccgtgc atgccggccc
    14281 gccacgacat cctcgtcggc gcgctcgact tcctgtggcg cccgtggggc tcggtcgaga
    14341 tctgggagga cgccgtcacc tacgacctgc ggcgggccgg tgtgaccacc atgttggtgt
    14401 cggaccaccc ccacctgttc gaggccggcg gcgagaacta ccacaccgac ttcggcgcct
    14461 ggcgctacgt ccgcggccac gagggcgacc cgtggcgaac cgcggcggac ccgtcgtggg
    14521 tcggcacccc ggcgctgccc gcccgcgagg gctggatccc ccacgcgtac gacgagagcc
    14581 ggacctggtt ccgttccgag gaggacttcc ccggcgcccg caccatggcc gaggccgccc
    14641 gctggctggc cgacgaggcg ccgtccgctg agcgcttcat gctgttcgtc gacgagttcg
    14701 acccccacga gcccttcgac gtccccgagc cgtggatgtc gcgctacgac cccgactggg
    14761 acgggccccg cgtcatctgg cccccgtacg cggtcgacgc cgtcgcctcc ggcgtcatcg
    14821 acgagcgcac cgcccgccac atccgcgcca actacggcgc caagctctcg ttcgtcgacc
    14881 accagttcgg acggctgctc gacgagctcg accggcggga cctctgggac gacaccgcgg
    14941 tgatcgtgtg caccgaccac ggccactacc tcggcgagca cgacatcttc ggcaagccgt
    15001 cgaccccggt gcgtgccgtg ctcgggcaca tcccgctgat ggtcgcctgg cccggacgcg
    15061 gccccggcgt ggtcgaccgg ctgaccacca ccgtcgacat ccacgccacg atctgcgacg
    15121 tgttcggcgt gacacccgag caccggacac acggccggtc gctggtgccg ttgctcgacg
    15181 acccggcggg cgggcccgcg cgggagtggg cgctgacggg catctggggc cagggggtga
    15241 ccgtcaccga cggtcggcgg cgctacctgc gcgcccccga cccggggaac cgtccgctgt
    15301 cgatgtggtc caaccggtgg tcgaccatgc cgatccacgc cttcccggag ctgcgcctgc
    15361 cccggccgga cggtcgggcg acgctcgagc ggatgccggg ctccgacgtc ccggtcatcc
    15421 accagccctt cggccccggc gaccccgtcc cctactgggc ggcggccgag ggccactcgc
    15481 agacgagcct gctgttcgac ctcgacgacg acccggccga gctccacgac ctgaccggca
    15541 ccgcgaccga ggtcgagatg gtcgacctgc tgcgcgccgc gctcggcgag atcgacgctc
    15601 cgggcgacca gctcgtccgg ctggggctga gctgagcgga cccgctcaga ggagtccgaa
    15661 ggccagacac cacaggagcg cgctgagcga caacccgtcc tgcacccggc cggcggagat
    15721 ctcaccgcgg agccggtcga ccggcaccca ttcgacccgc tcggcctcgg acgggtcggt
    15781 cggatctccc tcgtggaccg cgccgcgggc caggaacagg tcgaagcggt tgtcacccag
    15841 gccgttcatc gggaagtaat cgaccaggtg ctcgacggtg ccggcatcac cgccgatgcg
    15901 ccaccccgac tcctcgagca cctcccgggc cgccgcgtcg atcgaggcct cgccggggtc
    15961 caccccgccg ccggggacct cccagcccca cgtgtcggtg atgaaccggt gccgccacag
    16021 cagcagcacg cagggccgtg gcccggcgtc gtcggtctcg acgtcgtcga ggaccaccgc
    16081 ccccgcggcg gggccgcggg agcgcacgac gtggtgctcg aagcgacggg ccgggtgccc
    16141 cggcaccgcc ggcacctcga cgtcggtcag caccagccgg acccactccg actcgtagat
    16201 cggtcgctca ccgtggacgg tccaacgcac agccggaccg tagcgcccgc gtcggggtcc
    16261 tcagccgacg agcaggtgca ccgcgagtcc gagcaggagg ccggccgcga cgtagcgcgc
    16321 gacccgcagc gcgccggacg gaagggccga gccgacgacg tggccgaccc cgatccagcc
    16381 gtggtggacc gccagggacg ccagtcccat gcccgtgacc cagcccgcgg tgcccacgcc
    16441 cgctccgccg ccgacgacca ccccggtgta gacgagcagg gtcagcgggt tggccagggt
    16501 cagggccgcg aaccccccga ggagtcggcc cgtgccgtga tcgctgctcg actcgccggg
    16561 gccgccggcg ggggcgagca ccgggaccga ccgctcggag ccgcccgggc cggatgcgcc
    16621 ggcccgccgc accgagagca ctgcatggac cgcgatggcg gtcaccagca ccgctgcacc
    16681 gatccgcagc tgatcggcga ccggtgccag ccgggacccg agggcggtcc cggccaccgc
    16741 gaccaccacc gccagcacga cgtcgccgag ggccacgccg agcgcggcgg gagatccggc
    16801 ccgcagtccc cgacggacgc cgacgtcgac cagcagaagt gcgatcggtc cgacgaccac
    16861 gagcatcggc agtccggtgg ccaccccgag ggcgacgtca cgcacgaatc ccgtcatgga
    16921 ccgagcttgg cccggaatcg accgagcgat cggggttctt ccatggccgt taggcttgtt
    16981 caccatggat tccttggtga tcgatggaat cgaccgggcg atcctcgccg agttgcggat
    17041 cgacggtcgg gcgagctggc gggagctcgg cgagcgcgtc gggctggggc ccaccgccac
    17101 cgcggaccgg gtccgccggc tggagcgcca cg
//
LOCUS       DAOQOD010000054        17105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1180_length_17105_cov_11.336070, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000054 DAOQOD010000000
VERSION     DAOQOD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1180_length_17105_cov_11.336070"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1598
                     /locus_tag="PLZ72_07195"
     CDS             267..1598
                     /locus_tag="PLZ72_07195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNA36119.1"
                     /translation="MSQDSSPAPNPELFDTIVNLTKRRGFVYPSAEIYGGFRSTYDYG
                     PIGVLLLRNVKDAWWRSMVQERGDVVGLDASILSPPAVWEASGHLANFTDPLVDCTNC
                     NTRWRLDKLEDPEQCPGCGKRGTFTEPRQFNLMFKTQAGPVEDASAVAYLRPETAQGM
                     FINFKNVIDTTRVRPPFGIAQIGKSFRNEITPGQFVFRTREFEQMEMEYFVPPADAQR
                     WYEYWCNERYQWYLDHGMPADRLRVRAHDADELSHYSSGTSDVEFLFPWGWDELEGIA
                     NRGDYDLTQHAVHSGEKLEWYDQAANERWVPHVIEPAAGATRTAMAFLMAAYDEEEVR
                     GEKRVVLRFHPRIAPYKAAVLPLSKKPELTGPAGELAASLRRSFMCDYDETQSIGRRY
                     RRQDELGTPFCITYDFDTLEDRAVTVRDRDSMDQERVALAEVEAYLAEKLG"
     gene            1643..3466
                     /gene="dnaG"
                     /locus_tag="PLZ72_07200"
     CDS             1643..3466
                     /gene="dnaG"
                     /locus_tag="PLZ72_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNA36120.1"
                     /translation="MGIVDEDIARVRESTDLVAVVSGYTQLKRVGQRWSGLCPFHNEK
                     SASFSVNQAEGLYHCFGCKASGDAITFVREIEHLDFPGAVEWLAAKAGITLRYTERDE
                     SEGRKRQNRLFSLTEQACEWYHQRLRTGADAAAARSYLRSRGFDADEVARYRIGWAPD
                     DWDQLVRTLKASHDDLEAAGLGFLNRRNRLQDFFRGRILFPILDERDRVIGFGGRKLP
                     DADGPKYQNSRDNVLYNKSKALYAINWAKADAVASDQIVVCEGYTDVIGFSRAGIPRA
                     VATCGTALTEDHVRILKRFTRNIVLAYDADEAGQSAAERVYAWEAAHDLRFSVLELPA
                     GSDPDDLARTDPDALRSAVEHPRPFLEFRVTRVLGAADLSGPEGRARAAESALEVIAQ
                     HPDPLVRDQYVMELADTCRIDATQLRVRLEDVRRNPRPPEPERRRRGPDRDQDRGDDP
                     GSRPAPPRRPPLGDGVEREVLRLAVNQPELAAPFLEDSFFRHPTARAALDQLRRNDTL
                     AQAVEDAPPEVADLLTELAVEESTSEPGDVLARFASEVGRRELAELESEARASPDPLA
                     YSDVVAWLKVTLDHLRHPRAEVETVTQLLAFLRDRHQHARG"
     gene            3913..4818
                     /gene="rpoD"
                     /locus_tag="PLZ72_07205"
     CDS             3913..4818
                     /gene="rpoD"
                     /locus_tag="PLZ72_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNA36121.1"
                     /translation="MYLREIGQVPLLTAAEEVDLAQRIEAGVKAEERMADLAASGESV
                     DFEERRRLDRFRRDGERAKDELTQANLRLVVSIAKRYLGRGMHILDLVQEGNLGLMRA
                     VEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVESINKVHRQQRQLIQE
                     LEREPTVEELAAKCELKPEKVREILRISQDPLSLDSPVGEEDDSYLADFIADNDAVMP
                     ADVATANSLSEQIMSALDDLNDREKQVVRMRFGLDGGQPRTLEEVGREFGVTRERIRQ
                     IESKTLAKLRHPRSREKLEDYLKAD"
     gene            4896..4968
                     /locus_tag="PLZ72_07210"
     tRNA            4896..4968
                     /locus_tag="PLZ72_07210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4929..4931,aa:Asn,seq:gtt)
     gene            5088..7565
                     /locus_tag="PLZ72_07215"
     CDS             5088..7565
                     /locus_tag="PLZ72_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA36122.1"
                     /translation="MTEVDAQWVPERSDPTGPDRAALIEDLPDAVLVMDHQARLLWGN
                     RAAEDLFGRSLEESVGLDCITLVHPSDLEMAAVSLAGMQSERVGQPLELRVSTPEGWR
                     RMELVGHSRADDIILVFRDLTDRHRWEVASDNLDVLRTVLQHISTVVMVIEPDGRVRS
                     SSAALTRLLGLDQLEVEGRHLSGLVSSDDRVAVGRAIREALDAPVGASVAVDVGARRA
                     DDSFLPVTFSLVNLVDDPTVGGLVMTVHDISRSSAAEAALLETNAVLSATLESVADGI
                     VATDGVGIRTCNRQFFEIWEIPEESPARSDIYALTDHIRSMVEDPDALVARIAAIDAD
                     LAATSEDIIEFLDGRVIERRSRPRLIDGRAAGRVWSFRDVTATRRLQEELSHQAVHDP
                     LTGLANQVLFAQRLEAAVAGAGEGAEVAAMFVDLDNFKAVNDTLGHSSGDRLLVEMAE
                     RLRSSVRPGDTVARLGGDEFALLLVDLDGEEAAITVARRLIENVSRPIDLLAETVVVG
                     ASIGVAVTDRPVEPDSLLRSADLAMYHAKQSGRNQFRLYTSDMAHTSGGRGNADARLR
                     GAAARGELVVHYQPVVDHARDDAIVGLEALVRWNHPDRGLVMPGDFIPYAEASGLIDE
                     IGLHVLEEACRTAAGWQDYGPDAPLVSVNLSPHQLVDEGLPDRVGAVLAAVGLAGDRL
                     VLEFTESSLMQDPSVVARQIRAIRRHGVHIAIDDFGTGHSSLARLQQFPINTLKIDRA
                     FVHQVEDHTGRSLVRAIVQLAHTLNMLTVAEGVETAIQQQRLDEVGVDLAQGFLYHRP
                     LPAEEIAELLASCRGGLGRAAAPAPLP"
     gene            7572..8249
                     /locus_tag="PLZ72_07220"
     CDS             7572..8249
                     /locus_tag="PLZ72_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNA36123.1"
                     /translation="MTQVRPPSVRAGSRRLGVILGAAGVFGASSAVAARGVPESEVAA
                     FRALNELPAWVTPVLWPPMQLGSLWGPVAAGVLTWRRSHAWRPTAGVVIGGVAAWQLA
                     KVVKSFVQRGRPADELDHISWRPGTPREGLGFVSGHTTVAFAMVTALWPYAGRAQRLG
                     LLAVAAVVAGSRIQVGAHLPVDAVGGAALGVLVGETWTLLAGTGPADATTGSGPTQAP
                     PAGGSEQ"
     gene            8261..10183
                     /locus_tag="PLZ72_07225"
     CDS             8261..10183
                     /locus_tag="PLZ72_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNA36124.1"
                     /translation="MSAIVVVVVATVTTLLGSRLLGVRRGWVRQLLAGAIGWLVALVV
                     AVGLLDWDWDADELLAYTAVLAVPASMALMLAFDLVARPGTLAQGDRAGLIEAPSPIT
                     DLRSRIAPIARYRELLAILRRHQLLGHLNGRRDRTGTDQDPPEVRLREALEDAGGVYV
                     KLGQIAATRIDLLSPKVCAELGKLQSRSVPVPADDVRAVIENAYGRPADEVFERFDWE
                     PLAAASIGQTHTARLRDGTEVVVKVQRPGVEVTIERDLAALDQLARLAEKRTVAGRDL
                     RVSRLAAEFGRSLRSELDFLDEAGATATMHHLLEGSPIAVPRAFRDLSTRRVMVQERL
                     VGVPLSELDPDRDIDRDALARQLLSVFIEQLLSVGIFHADPHPGNVMVLEDGTLGLID
                     FGAIGRLDSIQIQAVVDMLIALTRGDASLLRDGVERVADVGVHVSRDRLERSLARLLA
                     DHVGTGGSVDVRALADLVPVLGEFEITLPGDLVVLMRAMVTLDGTLGVLSPGFSVTAA
                     GKELAQREAAERADGDPQQLITEALVAELPVLRRLPADVGRTLALASRGNLTFRTVAA
                     EDESRFTRTLWNRTLLAGIGLGLALVSVLLMGLEVGPLVGGDTRLVVLLGAGGLAFGS
                     VLVMRVVAAVVRDGTV"
     gene            10217..10289
                     /locus_tag="PLZ72_07230"
     tRNA            10217..10289
                     /locus_tag="PLZ72_07230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10250..10252,aa:Ile,seq:cat)
     gene            10528..10635
                     /locus_tag="PLZ72_07235"
     tRNA            10528..10635
                     /locus_tag="PLZ72_07235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10578..10580,aa:Gly,seq:acc)
     assembly_gap    10732..10831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10912..13335
                     /locus_tag="PLZ72_07240"
     CDS             10912..13335
                     /locus_tag="PLZ72_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNA36125.1"
                     /translation="MPIHAVSQMDGRIDRRVSRWRVQTFGPADEHPYRRRTSDWIRLI
                     VAVAVVAWLATGADQIGDVEQAVFEAFNQLPDSLDDLFRLLYGLGTLWGLGLVVVAAL
                     IGRRWRLARDLVLAGLLAAALARSIGVLVDGDSASEAVQATVRLGSSSPAFPATRLAV
                     IVAVVAAAAPYVTRPTRRTGRLFAVVMSLAAMYLGVALPDAVVAGAVLGWGCGALVHL
                     LFGSPGGRPTLPQMRASLAELGVDVEDLDLAPEQPDDATLMTASTEDGPLRIRVLGRD
                     EADAQVLGRIWRSIVFKSPPGRLHLTRIEAVEHEAYAVLLAAQAGVRTPEVVVTGTAG
                     PGAAVYVEHPVDGRPLSELGADELTENRLEQLWRQVDALHGAGVVHLDLTLDHVVLTG
                     DGDIALTSFGSATVGDRHDYRLHDVATLLVATSEVVGADRAVAAAAFGIGRERLAAVL
                     PVLQSAVLPSALRPGSRSERSAFTDRLSVLRSTAAAAVEVEPPRLTEMHRISGATLAM
                     VIGTFLGVAALLSQVGDPQELWDTMAAADWEWVSLAIVVSLLTNFSTAMALMGSVPIR
                     IPLLRTAELQLSLSFANLAVPTVGGLASQVRYLQKQGVDLAGAVAAGGVVSGVANVVV
                     TSSVCLLAILLSPAKITTEPIEPNQLLGLLLIAAVIIGAVCGIVFGVPRIRAHVLAPV
                     RNAWAIVSSALRSPRQLGQLVFGWAANALMYAFVLYCCVAAFGPPVNFWTIVLINTGV
                     STLAFAVPVPGGATAVSSVGVAGLLTAVGASQEVAVAASLAYQVTATFIPAVPGWFCF
                     RNLMAHDFL"
     gene            13373..14020
                     /locus_tag="PLZ72_07245"
     CDS             13373..14020
                     /locus_tag="PLZ72_07245"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HNA36126.1"
                     /translation="MTVDAWLLGAAMALSPLAVLASLLLLTTERGTTKAVTYAVGWVV
                     AVGSIGVLTVAAASTVHASPGSTTSSVSSWLYVLLGVVLVGWAIRRRVAARDGAPEPT
                     WMGRLDGMSPVVSLGFGLFMPPYAIAAAAVNSIVHGNAGGPAADVVVPVVTFTVVASL
                     GVIVPVAFALLSSSSDEVIARGREWLLARWPTVLFWMLVVVGGYLVAKGVYLLAT"
     gene            complement(14065..14631)
                     /locus_tag="PLZ72_07250"
     CDS             complement(14065..14631)
                     /locus_tag="PLZ72_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36127.1"
                     /translation="MAVIDVAGFVADLKNHAADHGFHIHDERHFVETYSMRQAWEVDL
                     HPEEACGGPVEFHLAVEVDPRTVLAFEDQVLELPEGDEPEERHQLPMTFTWSLPPLPH
                     CPDLLLLATELAGIGGPDLPLEVSAVDSFQSVTDPSERTLTVVARVPVSLSQVYMGEE
                     MLCDALERCLEVSRFLLDGAPSWMDGEV"
     gene            14702..15502
                     /locus_tag="PLZ72_07255"
     CDS             14702..15502
                     /locus_tag="PLZ72_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNA36128.1"
                     /translation="MSSDASDSRSVRTVQLLLDRTVAAVADALAEDRLDVDGVASDSS
                     WRDRGERPGQYRIDLVADAAALSVLRAAGVGVLSEESGMEDRGNDLVVVVDPVDGSTN
                     ASRGLPWYATSLCAVDAEGPLAALVVNLATGTRYSAVRGGGAFRDGRPVRVSSVQRPA
                     DALVVLNGYPSQHLGWRQYRALGATALDLCAVADGSVDATVDCAGDALGPWDYLGGVL
                     LIGEAGGVVADLDGRPLVELGHGVRRTPVSAATSQLLDALMSARGPTG"
     gene            15569..16096
                     /locus_tag="PLZ72_07260"
     CDS             15569..16096
                     /locus_tag="PLZ72_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA36129.1"
                     /translation="MIDAVQRVLLRVFRVLPRRVRRWIVRFGTPKYTVGAICIVQRRD
                     GAILLVRHSYAPRWGTPGGLAKRREPVDIAAARETLEEVNLAVELIGEPVVVVEPVPR
                     RVDVVFLARPAATAALDDVRPSSPEIVGTAWFLPDELPELQPETATALVALARDGRID
                     LAGSGLTPSSARPTR"
     gene            complement(16065..16907)
                     /locus_tag="PLZ72_07265"
     CDS             complement(16065..16907)
                     /locus_tag="PLZ72_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36130.1"
                     /translation="MLSGIADKVAVVTGAARPRSIGRATARRLAAEGARVACLDIARP
                     YDDFPDYAVATADDLDEIVEELRATGAEAVAVRADVSDEDQVTAAIAEVGERLGRVEL
                     LANVAGGSGAGMGAAPLLGLTKGEFQRVLDVNLVGTWLVSRACAEVMIEAGSGGRIVN
                     TSSQAGKVGWPMIGAYSAAKAGVINLTQVMAKEWAASQILVNAVCPGTVDTDLVNPGN
                     LFVDLMDAAQPDGFGGWVGREIPIGRMQTADEVASVIAFLMSDDARYITGEAVNVSGG
                     QMMA"
     gene            17026..17099
                     /locus_tag="PLZ72_07270"
     tRNA            17026..17099
                     /locus_tag="PLZ72_07270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17060..17062,aa:Val,seq:tac)
BASE COUNT         2313 a         5961 c         6115 g         2616 t
ORIGIN      
        1 ccgggggaac cgggggagcg ggaggagcgg ggggagccgc gggaggaccg ggcggcgggg
       61 tcggaacggc cgggggaacg tcgtcggacg gatcggtccc gcccggaggg ggcgggggca
      121 ggttgctcac cgcgtggacc tggtcggctg cgacttcatc cgcgtcacgg tagcgacggg
      181 cgcctcgtct gcggcggacc tgcccccacc gagcggacgg catcccgtgt gatcggggcc
      241 tcggcgcgcc ggtacgctcg gcccccatgt cccaggactc ctcgcccgct ccgaatccgg
      301 agctgttcga cacgatcgtc aacctgacca agcgccgcgg cttcgtctac ccctccgccg
      361 agatctacgg cgggttccgc tccacctacg actacgggcc catcggcgtg ctgttgctgc
      421 gcaacgtcaa ggacgcgtgg tggcgctcga tggtccagga gcgcggcgac gtcgtcggcc
      481 tcgacgcgtc gatcctgtcg cccccggcgg tctgggaggc ctccgggcac ctcgccaact
      541 tcaccgaccc gctggtcgac tgcaccaact gcaacacccg ctggcggctg gacaagctgg
      601 aggaccccga gcagtgcccg ggctgcggaa agcgtggcac cttcaccgaa ccccgccagt
      661 tcaacctgat gttcaagact caggccggcc cggtggagga cgcctcggcg gtggcctacc
      721 tgcggcccga gaccgcgcag ggcatgttca tcaacttcaa gaacgtgatc gacaccaccc
      781 gggtgcggcc gccgttcggc atcgcgcaga tcggcaagtc gttccgcaac gagatcaccc
      841 ccggccagtt cgtcttccgc acccgcgagt tcgagcagat ggagatggag tacttcgtgc
      901 cgcccgcgga cgcccagcgc tggtacgagt actggtgcaa cgagcgctac cagtggtacc
      961 tggaccacgg catgccggcg gaccggctgc gcgtccgggc ccacgacgcg gacgagctgt
     1021 cgcactactc gtcgggcacc tccgacgtgg agttcctgtt cccctggggt tgggacgagc
     1081 tcgagggcat cgccaaccgc ggcgactacg acctgaccca gcacgccgtg cactccggcg
     1141 agaagctcga gtggtacgac caggccgcga acgagcgctg ggtcccgcac gtgatcgagc
     1201 ccgccgccgg ggccacgcgc accgcgatgg cgttcctgat ggcggcctac gacgaggagg
     1261 aggtgcgcgg cgagaagcgg gtcgtgctgc ggttccaccc gcgcatcgcc ccgtacaagg
     1321 ccgcggtgct gccgctgtcg aagaagccgg agctcaccgg accggccggc gagctggccg
     1381 cgagcctgcg ccgttcgttc atgtgcgact acgacgagac ccagtcgatc gggcgtcgct
     1441 accgccgcca ggacgagctc ggcaccccgt tctgcatcac ctacgacttc gacaccctcg
     1501 aggaccgcgc ggtgaccgtc cgcgaccgcg actcgatgga ccaggagcgg gtggcgctgg
     1561 ccgaggtgga ggcgtacctg gccgagaagc tcggctgagt cgggccggac gggctgtcgg
     1621 gcggcgcttg tagtctccgc ccgtggggat cgtcgacgag gacatcgcac gggtccggga
     1681 gtcgacggac ctcgtcgcgg tcgtctcggg ctacacgcag ctcaagcggg tcggccaacg
     1741 gtggtccggc ctgtgcccgt tccacaacga gaagtcggcc agcttctcgg tcaaccaggc
     1801 cgaggggctc taccactgct tcggatgcaa ggcctccggc gacgccatca ccttcgtccg
     1861 cgagatcgag cacctcgact tcccgggtgc ggtggagtgg ctggcggcca aggccggcat
     1921 cacgctgcgc tacaccgagc gcgacgagtc cgaagggcgc aagcgccaga accgcctgtt
     1981 ctccctcacc gagcaggcct gcgagtggta ccaccaacgg ctgcgcaccg gagcggatgc
     2041 ggcggcggcg cggtcctatc tccggtcccg gggcttcgac gccgacgagg tggcccgcta
     2101 ccgcatcggg tgggcgcccg acgactggga ccagttggtc cgcacgttga aggcctccca
     2161 cgacgacctc gaggcggccg gcctcgggtt cctcaaccgt cgcaaccgcc tgcaggactt
     2221 cttccgcggc cggatcctgt tcccgatcct cgacgagcgc gaccgggtga tcggcttcgg
     2281 cggacggaag ctgcccgacg ccgacggacc gaagtaccag aactcgcgtg acaacgtcct
     2341 ctacaacaag tccaaggcgc tgtacgccat caactgggcc aaggccgatg cggtcgcctc
     2401 cgatcagatc gtcgtgtgcg agggctacac cgacgtgatc ggcttctccc gtgccggcat
     2461 cccgcgagcg gtcgccacgt gcgggacggc tctcaccgag gaccacgtgc ggatcctcaa
     2521 gcggttcacc cgcaacatcg tgctggccta cgacgcggac gaggccggcc agtccgccgc
     2581 ggagcgcgtc tacgcctggg aggccgccca cgacctgcgg ttctcggtgc tcgaactgcc
     2641 ggcggggtcg gaccccgacg acctggcccg caccgacccc gacgccctgc gctccgcggt
     2701 ggagcacccc cgaccgttcc tcgagttccg ggtgacccga gtgctgggcg ccgcggacct
     2761 gtccggaccc gagggccgcg cccgggccgc cgagtcggca ctcgaggtga tcgcccagca
     2821 ccccgacccg ctcgttcgag accagtacgt gatggagctg gccgacacgt gtcggatcga
     2881 cgccacccag ctgcgggtcc ggctcgaaga cgtccgtcgc aacccccgac cgcccgagcc
     2941 ggaacggcgt cggcgcggac ctgaccggga ccaggatcgg ggcgacgacc ccgggtcccg
     3001 tccggcgccg ccgcggcggc cgccgctcgg tgacggggtc gagcgggagg tgcttcgcct
     3061 ggcggtcaac cagcccgagc tggccgctcc gttcctcgag gactccttct tccgccaccc
     3121 gacggcacgc gccgcgctcg accaactgcg ccgtaacgac accctggccc aggcggtgga
     3181 ggacgctccg cccgaggtgg ccgacctgct caccgagctg gcggtcgagg agtcgacctc
     3241 ggagcccggc gacgtgttgg cccgcttcgc cagcgaggtc gggcgccggg agctggccga
     3301 gctcgagtcg gaggcgcgtg catcgccgga ccccctcgcg tactccgacg tcgtcgcctg
     3361 gttgaaggtc acgctcgacc acctgcgcca ccccagagcc gaggtggaaa cggtgaccca
     3421 gttgctagcc ttcctgcggg accggcacca gcacgcaagg ggttgatcgg attgtccatc
     3481 tgggagcagg tgccgacgga ggagatcgac gatctccaac gacgggccga cgcgagcgga
     3541 gccgtcctgc cgctggacga cgcgctgagc gcgctccgga tggagatgac cgatcaggtc
     3601 ctcgacgaca cccggacgtt ctgggacggt cgaggggtcc ggatcgacgc cgaggtcgtc
     3661 cccgacgaca tcgacctgct gccggccgac gacccggccg acgaccccga gcaccgcgtc
     3721 gtggaccggt ccgacgatgt cgacgacagc ggtgacgagc cggccgaggt cgcactcgtc
     3781 gagcccggtg agctggcgag cgagtcggtc cgccgtcggc ggctgctgcg caccacgggt
     3841 cgtcggggcc gccccgtgtc gctgtccggg acgggtgccg gaacggggag ctcgacggcg
     3901 gatcccgttc gcatgtacct ccgcgagatc ggccaggtcc ccctgctcac cgcggccgaa
     3961 gaggtcgacc tcgcccagcg catcgaggcc ggcgtcaagg ccgaggagcg gatggccgac
     4021 ctggcggcat cgggcgagtc ggtcgacttc gaggagcgcc gccgactgga ccggttccgt
     4081 cgcgacggcg aacgcgccaa ggacgagctg acccaggcca acctgcgcct ggtcgtgtcg
     4141 atcgccaagc gctacctcgg tcgcggcatg cacatcctgg atctggttca ggaggggaac
     4201 ctcggcctga tgcgggcggt cgagaagttc gactacacca agggcttcaa gttctccacc
     4261 tacgcgacct ggtggatccg ccaggcgatc acccgggcca tcgccgatca ggcccgcacg
     4321 atccgcatcc cggtgcacat ggtcgagtcg atcaacaagg tgcaccgcca gcagcgccag
     4381 ctcatccagg agctcgaacg ggagcccacg gtcgaggagc tggccgccaa gtgcgagctg
     4441 aagcccgaga aggtgcgcga gatcctccgc atctcgcagg atccgctctc gctcgactcg
     4501 ccggtcggcg aagaggacga cagctacctg gcggacttca tcgccgacaa cgacgcggtc
     4561 atgcccgcgg acgtggccac ggccaactcg ctgtccgagc agatcatgtc ggcgctcgac
     4621 gacctcaacg accgagagaa gcaggtcgtg cggatgcgct tcgggctcga cggcggccag
     4681 ccgcgcaccc tcgaggaggt cggacgcgag ttcggcgtca cccgggagcg gatccgtcag
     4741 atcgagtcga agaccctggc caagctccgc catccccgga gccgggagaa gctcgaggac
     4801 tacctgaagg ccgactgaga cccgatcacc cggttgggga ccgatggtcg gtggcgctag
     4861 tctccaaggc tgccgtcccc ccgggacggt cgccttccgg ggtagctcag ctggcagagc
     4921 agctgactgt taatcagcgg gtcgtgggtt cgagccccac ccccggagca gcacgcgtcc
     4981 gaccctcggc tactcgccga gggtcgttcc gcgtccgttc ggactctcgt ggtcccagcg
     5041 gcgggctgcg ttctcaatcc tggatcgtgc gtgccgatag tcccgtgatg accgaggtcg
     5101 acgcccaatg ggtcccggag cggtcggacc cgacgggtcc cgaccgggcc gcgctgatcg
     5161 aggacctacc cgacgccgtc ctggtgatgg accaccaggc gcggctgctg tggggaaacc
     5221 gggccgcgga ggacctgttc ggccggtcgc tcgaggagtc ggtgggactc gactgcatca
     5281 ccctggtgca cccttccgac ctcgagatgg cggccgtgtc gctggccggc atgcaatccg
     5341 agcgcgtcgg acagccgctc gaactgaggg tcagcacccc ggaagggtgg cgacggatgg
     5401 aactggtcgg ccacagccgg gccgacgaca tcatcctcgt gttccgcgac ctgaccgacc
     5461 ggcaccgctg ggaggtggcg tcggacaacc tcgacgtcct gcggaccgtg ctccagcaca
     5521 tcagcacggt ggtgatggtg atcgagcccg acggtcgcgt ccgcagctcg tcggccgcgc
     5581 tgacacgtct gctcgggctc gaccagctcg aggtcgaggg ccggcacctg agcggcctgg
     5641 tctcgagcga cgatcgggtg gcggtggggc gcgcgatccg cgaggcgctg gacgccccgg
     5701 tgggagcgtc ggtggcggtg gacgtcgggg cccgtcgggc cgacgattcg ttcctgcccg
     5761 tcacgttctc tctggtcaac ctggtggatg acccgactgt cggcggcctg gtgatgaccg
     5821 tccacgacat ctcccggtcc tcggcggcgg aggcagccct gctcgagacc aacgcggtgc
     5881 tgtcggccac cctcgaatcg gtcgcggatg ggatcgtggc gaccgacggg gtcggcatcc
     5941 ggacctgcaa ccggcagttc ttcgagatct gggaaatacc cgaggagtcg cctgcccgca
     6001 gcgacatcta cgccctgacc gaccacatcc gctcgatggt cgaggacccc gacgccctgg
     6061 tggcccgcat cgcggcgatc gacgcggatc tggcggcgac gagcgaggac atcatcgagt
     6121 tcctcgacgg gcgggtcatc gagcgacgat cccgcccacg actcatcgac ggtcgtgccg
     6181 cgggtcgtgt gtggagcttc cgtgacgtca ccgcgacccg acggctccag gaggagctgt
     6241 cccatcaggc ggtccacgac ccgctgacgg gcttggccaa ccaggtgttg ttcgcccagc
     6301 ggttggaggc cgcggtggcc ggggccggtg agggcgctga ggtggccgcc atgttcgtcg
     6361 acctcgacaa cttcaaggcc gtgaacgaca cgctcgggca ctcgagcggt gaccggctgc
     6421 tggtcgagat ggccgagcgg ctccggtcat cggtccgacc cggcgacacg gtggcccggc
     6481 tcggcggcga cgagttcgcg ctcctgctcg tcgacctcga cggcgaggag gccgccatca
     6541 ccgtcgcccg acgtctgatc gagaacgtgt cgcggcccat cgacctgctg gccgagacgg
     6601 tggtcgtcgg agccagcatc ggcgtcgccg tcaccgaccg tccggtggag cccgactcgc
     6661 tgctgcgcag cgcggacctg gccatgtacc acgccaagca gagcggtcgg aaccagttcc
     6721 ggctctacac gtcggacatg gcgcacacct cgggcggacg cggcaacgcg gacgcacgtc
     6781 tgcgtggggc ggccgcacgc ggcgagctgg tggtgcacta ccagcccgtc gtcgaccatg
     6841 cccgcgacga tgcgatcgtc ggtctcgagg ccctggtccg atggaaccat cccgatcggg
     6901 gactggtcat gcccggggac ttcatcccct acgccgaggc cagcggtctg atcgacgaga
     6961 tcggactcca cgtcctcgag gaggcctgtc ggacagcagc cggttggcag gactacggcc
     7021 ctgacgcccc tctggtcagc gtgaacctgt caccgcacca gctcgtcgac gagggacttc
     7081 cggaccgggt gggagccgtg ttggccgccg tgggcctggc cggtgaccgt ctcgtgctcg
     7141 agttcaccga gagctccctg atgcaggacc cgtcggtggt ggcccgtcag atccgcgcca
     7201 tccgacgtca cggcgtccac atcgcgatcg acgacttcgg caccggtcac tcgtcgctcg
     7261 cccggttgca gcagttcccg atcaacaccc tcaagatcga ccgggccttc gtccatcagg
     7321 tcgaggacca cacgggccgg tcgctggtcc gagcgatcgt gcagctcgcc cacactctca
     7381 acatgctcac ggtggccgag ggcgtggaga ccgccatcca gcagcagcgc ctcgacgagg
     7441 tgggtgtgga cctggcccag ggcttcctgt accaccgtcc gctgcccgcc gaggagatcg
     7501 ccgagctcct cgcgagctgt cgcgggggtc tgggccgagc agcggcgccc gcaccgctac
     7561 cctgagccgc catgacgcag gttcggcccc cttccgtccg ggccggatca cgacggttgg
     7621 gtgtgatcct cggagccgcg ggggtgttcg gggcctcgtc agcggtcgcg gcgcgagggg
     7681 tgcccgagtc cgaggtcgcg gcctttcgtg ccctcaacga gctgcccgcc tgggtgaccc
     7741 ctgtgctgtg gccgccgatg cagctcggca gcctgtgggg cccggtcgct gcgggggtcc
     7801 tgacctggcg acggtcgcac gcctggcgtc cgaccgccgg ggtggtgatc ggcggcgtcg
     7861 ccgcgtggca gctggccaag gtggtcaagt ccttcgtgca gcgcgggagg ccggccgacg
     7921 agctcgacca catctcctgg cgccccggca ccccacggga gggcctcggg ttcgtctcgg
     7981 gccacaccac cgtcgcgttc gccatggtca ccgcgctgtg gccgtacgcg gggcgggcgc
     8041 aacggctcgg gctgctggcc gtcgccgcgg tcgtggccgg gtcgcggatc caggtcgggg
     8101 cgcacctgcc ggtcgacgcc gtcggcggtg ccgctctcgg cgtcctggtc ggcgagacct
     8161 ggacgctcct cgccgggacc ggaccggctg acgcaaccac ggggtcgggt cccacgcagg
     8221 cgccaccggc aggaggttcg gagcagtgat caggttcctg gtgagcgcga tcgtcgtcgt
     8281 ggtcgtcgcc acggtgacca cgctgctggg cagtcggctg ctcggagtgc ggcggggctg
     8341 ggtgcgccaa ctgctggccg gggcgatcgg atggctggtg gcgctggtgg tcgctgtcgg
     8401 tcttctcgac tgggactggg acgcagatga gctgctggcc tacacggcgg tcctggccgt
     8461 gccggcgtcg atggcgctga tgctggcgtt cgacctggtg gcacgacccg ggaccctggc
     8521 ccagggggat cgggccgggc tgatcgaggc gccgtctccg atcacagacc tccggtcccg
     8581 gatcgcgcct atcgcccggt accgcgagct gttggccatc ctgcggcgcc accagctcct
     8641 cggacacctc aacggtcgcc gggaccgcac cggaaccgat caggatccgc ccgaagtgcg
     8701 gttgcgggag gcgctcgagg acgccggcgg cgtctacgtg aagctcggcc agatcgcggc
     8761 gacccgcatc gacctgctgt cgccgaaggt gtgcgccgag ctgggcaagc tccagagccg
     8821 gtcggtcccg gtgcccgcgg acgacgtgcg ggccgtgatc gagaacgcct acggccggcc
     8881 ggccgatgag gtgttcgagc gcttcgactg ggagccgctg gccgcagcgt cgatcggtca
     8941 gacccacacc gcccggttgc gagacgggac cgaggtggtg gtgaaggttc agcggccggg
     9001 cgtcgaggtc accatcgaac gggatctcgc cgcgctggac cagttggcgc gcctggccga
     9061 gaagcgcacc gtggccggcc gcgacctgcg ggtgagtcgc ctggccgccg agttcggtcg
     9121 cagcttgcgc tccgagctcg acttcctcga cgaagcaggc gcaaccgcca ccatgcacca
     9181 cctgctcgag ggcagcccga tcgccgttcc ccgggccttc cgggacctga gcacgcgtcg
     9241 ggtcatggtc caggaacgtc tggtcggggt tccgctgtcg gagctggacc ccgatcggga
     9301 cattgaccgc gacgccctcg cccgtcagct gctgtcggtg ttcatcgagc agctcctgag
     9361 cgtggggatc ttccacgccg acccccaccc gggcaacgtc atggtgctcg aggacgggac
     9421 gctcgggttg atcgacttcg gagcgatcgg ccgactggac tcgatccaga tccaggcggt
     9481 cgtcgacatg ctgatcgccc tcacgcgggg cgacgcctcg ctgctgcgtg acggggtgga
     9541 acgggtggcg gacgtcggcg tccacgtgtc gcgggaccgg ctcgagcgat ccctggcgcg
     9601 gctgctcgcg gatcacgtcg gcaccggggg atcggtcgac gtgcgggccc tggccgacct
     9661 ggtcccggtc ctcggcgagt tcgagatcac gctgccgggc gacctggtgg tcctgatgag
     9721 ggcgatggtc accctcgacg gaaccctggg cgtgctcagc cccggcttct cggtcaccgc
     9781 ggcgggcaag gagctcgcac agcgcgaggc cgccgaacgg gccgacggcg acccccagca
     9841 gctgatcaca gaggcgttgg tggccgagct gccggtcctg cgacgcctac ccgctgacgt
     9901 ggggaggacg ctcgctctgg cgtcgagggg caacctcacg ttccgcaccg tcgccgccga
     9961 ggacgagtcc cggttcaccc gcacgctgtg gaaccggacc ctgctcgccg gaatcggcct
    10021 cggcctggcg ttggtgtcgg tgctgctcat ggggttggag gtcggtccac tggtcggggg
    10081 cgacacccgg ctcgtggtgc tgctcggcgc cggcggtctg gcgttcggct ctgtgctcgt
    10141 catgcgggtg gtcgccgctg tcgtgcgcga cggcacggtg tgagtcggcc ggtggtgggg
    10201 ccggtacgct cccgccgggc cggtagctca gtggtcagag caggcgactc ataatcgctc
    10261 ggtcccgggt tcgatccccg gtcggcccac gaaggacacg atcacctggt cgtcaccgtt
    10321 gcccgcgggt acccgtgtgc aacgactgcg tagccgccga caatgaggaa cctgacctca
    10381 cgcctgtcgc agcactcgat gaactcgacg aagtccggac tgaggaactt catccgcgca
    10441 ctccagactg agttcggtcg ccatcgccag acgttcctgg gcgggtcgag tgcgccagta
    10501 ctcgacccac gagtcatcgg ggtcaccgag acgtcgcttg gtggcgacgg cgacgacctg
    10561 atgcatgcga ccagcctacc gacgctcgcg tcgctggcgg attggtcccg ggttcgattc
    10621 ccggccggct caccagatcc gacgatgttg tagctggtgg tcgctggggt ggcgtgtggg
    10681 ctacaagttc ggcggcgggt ggcgatgttg tagctggcgg tcgctcgggt gnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtgtgggc tacaagttcg gccggagtgg
    10861 gcgggcgcgt ggctccgcgg tgcggccgac acgctcccgt agcctgcggc ggtgccgatc
    10921 cacgcggtct cccagatgga cggccggatc gaccggcgcg tgtcgcgatg gcgggtgcag
    10981 accttcggcc ccgcggacga gcacccgtac cgccgtcgga ccagcgactg gatccggctg
    11041 atcgtcgccg tcgctgtcgt cgcctggctc gctaccggtg ccgaccagat cggcgacgtc
    11101 gagcaggccg tcttcgaggc gttcaaccag ctgcccgact ccctcgacga cctgttccgc
    11161 ctcctctacg ggctcgggac gctgtggggc ctcggcctgg tcgtggtggc tgccctgatc
    11221 ggccgacggt ggcgactggc ccgggacctc gtcctggccg gcctcctcgc cgccgcgctg
    11281 gcgcgttcca tcggcgtgct cgtcgacggc gacagcgcga gcgaggcggt ccaggccacg
    11341 gtccgactcg gctcgtcgag cccggcgttc ccggccaccc gcctggcggt catcgtcgcc
    11401 gtcgtcgccg cggccgcccc ctacgtcacc cggccgaccc gtcgcaccgg acgcctgttc
    11461 gcagtcgtga tgtcgctcgc ggccatgtac ctgggcgtcg ctcttcccga cgcggtcgtc
    11521 gcaggtgccg tcctgggctg ggggtgcggg gcgctcgtcc acctgctgtt cggctcgccc
    11581 ggaggtcgtc ccacgttgcc gcagatgcgg gcatcgctcg ccgagctcgg tgtcgacgtc
    11641 gaggatctgg acctcgcacc ggagcagccc gatgacgcaa ccctgatgac cgcgtcgacc
    11701 gaggacgggc cgctgcggat ccgggtcctg ggtcgcgacg aggccgacgc ccaggtcctc
    11761 ggccggatct ggcgctcgat cgtgttcaag tccccgccgg ggcgactgca cctgacccgc
    11821 atcgaagcgg tcgagcacga ggcctacgcc gtgctgctgg ccgctcaggc cggggtccgg
    11881 accccggagg tcgtcgtgac cgggaccgcg ggtccgggtg ctgcggtgta cgtggagcac
    11941 ccggtggacg gtcgccccct gtccgagctc ggtgccgacg agctgaccga gaaccgactc
    12001 gagcagctct ggcgacaggt cgatgccctc cacggagccg gggtcgtgca cctcgacctc
    12061 acgctcgacc acgtggtgct gaccggcgac ggggacatcg ccctcacctc gttcggttcc
    12121 gccaccgtcg gggaccgcca cgactaccgg ctccacgatg tcgccacgtt gctggtcgcc
    12181 acctccgaag tcgtcggcgc ggaccgggcc gtggccgccg cggctttcgg catcggccgg
    12241 gagcgcctgg ccgcagtcct gcccgtactg cagtccgcgg tgctgccgtc tgcgctgcgg
    12301 cccgggtccc gttcggagcg ttcggcgttc accgaccggc tgagcgtgct gcgctccacc
    12361 gccgcggcag cggtcgaggt ggaaccgccg cgactgaccg agatgcaccg gatctcgggc
    12421 gcgaccctgg ccatggtcat cggcaccttc ctgggtgtcg ccgccctgct cagccaggtc
    12481 ggcgaccccc aggagctgtg ggacacgatg gccgccgcgg actgggagtg ggtgagcctg
    12541 gcgatcgtcg tgtcgctcct caccaacttc tccaccgcca tggccctcat gggcagcgtg
    12601 ccgatccgga tcccgctgtt gcgcaccgcg gagctgcagc tgtcgctgtc gttcgccaac
    12661 ctggccgtcc ctaccgtcgg cgggctcgcg tcgcaggtgc gctacctgca gaagcagggt
    12721 gtggacctgg ccggggcggt ggcggccggg ggagtcgtgt cgggcgtggc caacgtggtc
    12781 gtgaccagct cggtgtgcct gctggcgatc ctgctgtccc cggcgaagat cacgaccgag
    12841 ccgatcgaac cgaaccagct gctcgggctg ctgctgatcg cagcggtgat catcggagcg
    12901 gtgtgcggga tcgtgttcgg cgtgccccgc atccgggccc acgtcctggc cccggtccgc
    12961 aacgcctggg cgatcgtgtc atcggcgctg cgttcgcccc gccaactcgg ccagctggtg
    13021 ttcgggtggg cggccaacgc cttgatgtac gcgttcgtcc tctactgctg cgtcgccgcg
    13081 ttcggaccgc cggtcaactt ctggacgatc gtgctcatca acaccggggt gtcgaccctg
    13141 gcgttcgcgg tgccggtgcc cggcggggcg accgcggtca gctcggtcgg ggtggccggt
    13201 ctgctcaccg cggtcggggc gagccaggag gtggcggtgg ccgcgtcgct ggcctaccag
    13261 gtcaccgcga cgttcatccc ggcggtgccc gggtggttct gcttccggaa cctgatggcc
    13321 cacgacttcc tgtgaccgtg gacgaccggg tggccgtggg cggtgctgtc gcgtgaccgt
    13381 cgacgcgtgg ctcctcgggg cggcgatggc gttgtcgccc ttggcggtgc tggcgtccct
    13441 gctgctgttg acgaccgagc ggggcacgac gaaggcggtc acctacgccg tcgggtgggt
    13501 ggtcgcagtc ggttcgatcg gcgtgctcac cgtcgccgcc gcctcgacgg tgcacgcgag
    13561 tccgggaagc accacgtcga gcgtgtcgtc ctggttgtac gtccttctgg gcgtggtcct
    13621 cgtcggatgg gcgatccgcc gtcgggtcgc ggcgcgtgac ggcgcccccg aacctacgtg
    13681 gatggggaga ctcgacggca tgtccccggt cgtgtcgctc ggcttcggcc tgttcatgcc
    13741 cccgtacgcg atcgcggccg cagcggtgaa ctcgatcgtg cacggcaacg ccggcggacc
    13801 ggccgcggat gtggtcgttc ccgtcgtgac cttcacggtc gtggcgtcgc tcggggtgat
    13861 cgtgcccgtg gcgttcgctc tgctgtcctc gagctccgac gaggtgatcg cccgcgggcg
    13921 ggaatggctc ctcgctcgct ggccgacggt gctgttctgg atgctggtcg tcgtcggcgg
    13981 ctacctggtc gccaagggcg tgtacctgct cgcgacctga tcaccggaac ggtgtcaccg
    14041 agcgggtccg ctctcctctg tcgatcagac ctcgccgtcc atccaggacg gggcgccgtc
    14101 gagcaggaag cggctgacct ccaggcatcg ctccagggcg tcgcagagca tctcctcgcc
    14161 catgtagacc tggctgaggc tgaccgggac ccgagcgacg acggtcaggg tccgctcgga
    14221 cgggtccgtc accgactgga acgagtcgac cgccgagacc tcgagcggca ggtcagggcc
    14281 gccgatgccg gccagctcgg tggcgagcag cagcaggtcc gggcagtgcg gcagcggcgg
    14341 cagcgaccag gtgaaggtca tggggagctg gtggcgctcc tcgggttcgt cgccctcggg
    14401 cagttccagc acctgatcct cgaaggcgag cacggtgcga gggtcgacct cgaccgccag
    14461 gtggaactcg acgggaccgc cgcacgcctc ctcggggtgc aggtccacct cccacgcctg
    14521 gcgcatggag taggtctcga cgaagtgccg ctcgtcgtgg atgtggaagc cgtggtccgc
    14581 ggcgtggttc ttgaggtccg ccacgaaccc ggcgacgtcg atgactgcca tcgcccacca
    14641 gattgccagc cgggggcggc cccgtgaaac cacgttgcgc ggggaccggt accctcgatc
    14701 cgtgagctcc gacgcgtccg actcccgctc ggtccgcacc gtccagctgt tgctcgaccg
    14761 cacggtggcc gccgtggccg acgccctggc cgaggaccgc ctcgacgtcg acggggtcgc
    14821 gtcggactcc agctggcggg accgcgggga gcgacccggt cagtaccgca tcgatctggt
    14881 cgccgatgcc gccgcgctgt cggtgctgcg cgccgcaggc gtcggggtcc tcagcgagga
    14941 gtccgggatg gaggaccggg gcaacgacct ggtcgtggtc gtcgacccgg tcgacggctc
    15001 gacgaacgcc agccgggggt tgccgtggta cgcgaccagt ctctgcgccg tcgacgccga
    15061 gggtccgctc gccgcgttgg tggtgaacct ggccaccggg acgcgctact cggcggtccg
    15121 gggtggggga gcgttccgcg acggccggcc cgtgcgggtc tcgtcggtgc agcgaccggc
    15181 cgacgctctg gtcgtgctca acgggtaccc gtcgcagcac ctcggttggc gccagtaccg
    15241 ggcgctggga gcgaccgccc tcgacctgtg cgcggtggcc gacgggtcgg tcgacgcgac
    15301 ggtcgactgt gccggcgacg ccctcggacc gtgggactac ctgggcggtg tgctgctgat
    15361 cggcgaggcc ggcggggtgg tggccgacct ggacggccga ccgctcgtcg agctcggcca
    15421 cggcgtgcgc cgcacgccgg tctccgcggc cacgtcccag ctgctcgacg cgctgatgag
    15481 cgcccggggc cccaccggct gaccacgaac ccgtcggcgg tgccgatccg cccgaccggt
    15541 tcgagccgct gcgccgtatc gtggcggggt gatcgacgcc gtccaacggg tgctgctgcg
    15601 cgtgttccgg gtgctgcccc gacgcgtccg gcgttggatc gtgcgcttcg gcacccccaa
    15661 gtacaccgtg ggcgcgatct gcatcgtgca gcgtcgcgac ggggcgatcc tgctggtgcg
    15721 gcactcctac gcccctcggt ggggcacccc gggcgggttg gccaagcgcc gtgaaccggt
    15781 cgacatcgcc gcggcccgcg agaccctcga ggaggtcaac ctggccgtcg agctgatcgg
    15841 cgagccggtg gtggtggtcg agcccgtccc ccgccgcgtc gacgtcgtgt tcctggcccg
    15901 acccgcggcg accgctgcgc tcgacgacgt ccgacccagc tcgcctgaga tcgtgggcac
    15961 cgcgtggttc ctgcccgacg agctgccgga gcttcagccg gagaccgcga ccgcgctggt
    16021 ggcgctcgcc cgcgacggac gcatcgacct ggccggatcg ggcctcacgc catcatctgc
    16081 ccgcccgaca cgttgaccgc ctcgccggtg atgtagcggg cgtcgtcgga catcaggaac
    16141 gcgatcaccg acgccacctc gtccgcggtc tgcatgcgtc cgatcgggat ctcccggccg
    16201 acccagccgc cgaagccgtc gggttgcgcg gcgtccatca ggtcgacgaa caggttcccg
    16261 gggttgacca ggtcggtgtc gaccgtgccg gggcacacgg cgttgaccag gatctgcgac
    16321 gccgcccact ccttggccat cacctgggtg aggttgatga ccccggcctt ggccgccgag
    16381 taggcgccga tcatcggcca cccgaccttg ccggcctgcg aggacgtgtt gacgatccgt
    16441 ccgccgctgc cggcctcgat catcacctcg gcgcacgcgc ggctgaccaa ccaggtgccg
    16501 accaggttga cgtcgagcac ccgctggaac tcgcccttgg tcaacccgag caggggagcg
    16561 gcgcccatcc ccgccccgga cccgccggcg acgttggcga gcagctcgac gcgacccagg
    16621 cgttcgccga cctcggcgat cgcggcggtc acctggtcct cgtcggacac gtccgctcgc
    16681 accgcgacgg cctcggcgcc ggtggcgcgc agctcctcga cgatctcgtc gaggtcgtcg
    16741 gcggtggcca cggcgtagtc ggggaagtcg tcgtaggggc gggcgatgtc gaggcacgcg
    16801 acccgggccc cctcggccgc caggcgtcgg gcggtggccc gaccgatcga gcgggggcgg
    16861 gcggcgccgg tgaccacggc gaccttgtcg gcgattccgg agagcatgtt cggatggtac
    16921 gggattcgat tccgatgact gtcagcgcca ctggtggcgc gaggagtcat gagaatccgc
    16981 aggtcgatgg aggaggctgg gccgcaggta cgctcgtcga cttccgggcg tgtagctcag
    17041 ttggtcagag cacctgcctt acaagcaggg agtcgggggt tcgaagccct ccacgcccac
    17101 tccgg
//
LOCUS       DAOQOD010000055        17048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1189_length_17048_cov_12.577708, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000055 DAOQOD010000000
VERSION     DAOQOD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17048
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_17048_cov_12.577708"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLZ72_07275"
     CDS             complement(<1..583)
                     /locus_tag="PLZ72_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNA36131.1"
                     /translation="MRSPRRLLLITAIGLTAAVGLSGCGVAADDTAATVGGVTISATL
                     VNQLATDDAFMAAMTNQALQDQRTGVVEGPAARQVLAFLVSSEVLAQEVARWDATVSE
                     QDMAQGEATISQQAPELKGRARDSVLRYLVDRAALQARLGEIDPSSDKDMRRLYEGVP
                     AYWDQVCMTAVVVPAGGVADARAALRKGADLAEL"
     gene            complement(648..4148)
                     /gene="mfd"
                     /locus_tag="PLZ72_07280"
     CDS             complement(648..4148)
                     /gene="mfd"
                     /locus_tag="PLZ72_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNA36132.1"
                     /translation="MDDEPAPTAAPTGPPLAGLLGVLRDEPGLLGALGRRSTVLVLPD
                     AARAAALAGLTALSRRRPIVVAVPNVAEAERLAGDLTAFLPQDAVELFPSWETLPFER
                     VSPSIETMGRRLRVMGRLRCGDDTLQVVVAPARALAQRLGPGVEDTEPVRIVPGERLD
                     QQELVERLVLSGYRREYQVEHRGEVAVRGSIIDVFPSTADRPVRIDLWGDEVDRLAEF
                     SVADQRSGADLTHVELYPARELLPTAAVRERAERLLATDPWGREQWQRLADGEVFEGM
                     EAWLPWLVDHDGGPEHVLVDLLDDDGLVLLVEPRRLRDRVGDVLAEEADLGATLARTW
                     GLDDRVDLPRLHLDMERLLARTEAPTWSVAAVPDSPDTPAVASSGWPPAVGGGEALIG
                     QLRQLVADGYRVVVCADGSGSATRLAQLIAESGLSFAVLEPGTDGEVDARALLAPGGT
                     VVVAPIDRGCILPGVKVALLAEADLTGRRRTHRPPKAPRRDAQRFYEDLKVGDYVVHQ
                     HHGVARFAGMVERTMGGAARDYLLLEYRGDDKLYVPSEQIDAIRLYSGGETPSLNRMG
                     GADFARTKAKVRSAVAEIAQELVVLYQRRRAAAGHPSGPDTPWQREMEAAFPFQETPD
                     QLSAIRDVKEDMESTVPMDRLVCGDVGFGKTEVAVRAVFKAVQDGKQAAVLVPTTLLA
                     QQHFQTFADRYAGYPVRVEVLSRFLTNAQAREVTEGLANGSVDVVIGTHRLLSGDIAF
                     KDLGLLVVDEEQRFGVSHKEAIKQFKNDVDVLTLSATPIPRTLEMSLTGIRDLSLLNT
                     PPAARQPILTYVGEYDERAVAEAIRRELLREGQVFYVHNRVRDIEEHAARIRDLVPEA
                     RVAVAHGQMDEGTLERVVLDFWEGEFDVLVCTTIIESGIDMPTVNTLVVERADLLGLG
                     QLHQIRGRVGRSGQRAYAYLFFPPDRQLSEEAYERLKTIGETTELGSGFRIAMRDLEI
                     RGAGNLLGTGQTGHVAAVGYDLYVQMVNEAIAQLNGEPVPEPTEITLELPLAAHLPAD
                     YVERDDLRLDAYRRLAGVQSQADVDDIQAEWEDRFGPVPDPAANLLAIARLRAECVRT
                     GVEEIAVTKASALAGPGWVARLAPVRLAQSKQMRLARLYKGAVYKEETSQLQLPVRAG
                     ATLPADLVEALTQLLPDAAR"
     gene            4191..5051
                     /locus_tag="PLZ72_07285"
     CDS             4191..5051
                     /locus_tag="PLZ72_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36133.1"
                     /translation="MAVVLATLSAIAFGVGDFLGGLSARRMAAVATALGTQVVGLVLI
                     LALSPVIGGTVTATDLGWGMAAGVVGSSALMVFYWALGAGQMSVVAPISAVTSALVPL
                     CFGLLDGERPGAVAMTGALLALPAIVLISREPSDPVGADERDDPPHRAPGSRVVAASA
                     VAGAGFGAFFVLISRSGEDSGLWPLASARSVAVVVVGAAVLLTGVRRVERTGVQLAIG
                     AGSFDVAANALFLLASRQGLLTLVGVIGSMYPASTIVLARVVLRERIARHQMTGLALA
                     AASLVAVTIG"
     gene            complement(5067..5651)
                     /gene="pth"
                     /locus_tag="PLZ72_07290"
     CDS             complement(5067..5651)
                     /gene="pth"
                     /locus_tag="PLZ72_07290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNA36134.1"
                     /translation="MPWDLLVLGLGNPGDRYAGTRHNVGAEAVELLARRHGTRLKVDG
                     RVNARVADIRLGDIRVVLAVPTTYMNESGQAAVALVRRYGIEDMAHLVVVHDELDLDP
                     GRLKLKLGGGLAGHNGLRSLDAHLHSADFVRVRMGVGKPPGGSDRGADWVLSKVRGRV
                     RDELDVMIAEAADAVADIATDGLDAAMQRWNQRG"
     gene            complement(5651..6301)
                     /locus_tag="PLZ72_07295"
     CDS             complement(5651..6301)
                     /locus_tag="PLZ72_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNA36135.1"
                     /translation="MSEVTLTASTGRETGTRPSRRLRREGLIPGVVYGEGKDATVLSV
                     DRKELRRALSTDAGQNALISLTVDGTTYLTVVKELQRHPVRREVTHVDFLTVDPDAAI
                     EVEVPIHLVGEAKEVSQNGGITEQRLMALKVLVKPGAIPDGIDADITDLTLTTTITAA
                     DLVLPAGVELVTDPEQAVVTAELTRAAVTARNEGDDSEGGDGDGDAEAAGDGDAEG"
     gene            6591..7508
                     /locus_tag="PLZ72_07300"
     CDS             6591..7508
                     /locus_tag="PLZ72_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36136.1"
                     /translation="MTAGERLGVGGCPLFPRDHAFHASVAALPVRADSAEVVAALGGR
                     SLAVRPAFSASVWQGSRSGIPVNVVDSRLTAPVDVDGGTYAYLSDLSGHPIPASPRLE
                     GWPGMAWDRHLLIVDSATCTASEMFFVTPPGLSPTRRWGAETAVRIDLGSNSPRAEGT
                     ATASGTSMLAGMVRYDEVARDRLDHAIGVSVPTIRAGAVRWPATHTDGRSEDPRAPEM
                     GSWLRLRRDVDLSALGPQARVVARALRDHGAVVVDTNHDGLALSGEPDERWDDADLAT
                     LRTLTAADFEVVDPDPMRAEPGTESYRIR"
     gene            complement(7536..8519)
                     /locus_tag="PLZ72_07305"
     CDS             complement(7536..8519)
                     /locus_tag="PLZ72_07305"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNA36137.1"
                     /translation="MELVTKKRLTMVAGRVNRELAEEIAERLGISLAPMEIAEFANGE
                     LHCKFGESIRGADVFIFQSHCSTSELSVNDALMEQLIMVDAARRASAKRITIVAPFYG
                     YGRQDRKAEGREPITAKLLANMFEVAGAKRIVSVDLHSGQIQGFFDGPVDHLTAMPVL
                     VQWMADNLGEDLVVVSPDAGRVKVAERYANALHADLAIVHKRRVKGAKNQVEAKDVVG
                     EVEGRTCVLIDDMIDTAGTIVAAAEQLAEHGASSVYCAATHGVFSGPAIDRLKNSNLE
                     KVVVTNTLPLPPEKHIDKIEILSAAGIIADAIDAVFEDTSVSEIFGGQNQH"
     gene            complement(8549..9670)
                     /locus_tag="PLZ72_07310"
     CDS             complement(8549..9670)
                     /locus_tag="PLZ72_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNA36138.1"
                     /translation="MNETPIDTAAGPPPPLAAVVLAAGEGRRMQSSRPKPLHRLCGRP
                     MLMYVIDSLAGTDTSRVAIVVGHKGDRVAKTMLEHVGDVAVDFVEQRVQRGTGDAALV
                     GLVGLPDDDDDGDVLVLPGDAPLLRPETIASLVDHHRRTGAAATLLTAVMDDPTGYGR
                     VIRSGDGSVERVVEHTDADAEERAVDEINTSIYCFRRNLLAPALRRVQPDNAQGEYYL
                     TDVVSVLSGTGHRVEAVVAPDAAETQGVNDRVQLAAAEAELRRRTNEALLRSGVTMLD
                     PASVFVDTTARVGQDVTLFPGVILQGATVVGDGTELGPNTRLVDCIVGDDCVIEQSTG
                     RSARIGDRCTVGPFASLGPGAELASDVTTGPFYTADTAT"
     gene            complement(9703..9777)
                     /locus_tag="PLZ72_07315"
     tRNA            complement(9703..9777)
                     /locus_tag="PLZ72_07315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9743..9745),aa:Gln,seq:ttg)
     gene            complement(9842..10903)
                     /locus_tag="PLZ72_07320"
     CDS             complement(9842..10903)
                     /locus_tag="PLZ72_07320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA36139.1"
                     /translation="MTSGARAGSPEVAIPASETVPGLVTVAIPARNESASIRRVLDAV
                     RSQTYPDLQIVVVDGSSEDDTAAIVADVARSDPRVELVANPDRVIPRAMNLALAHARG
                     EWFVRVDAHCRIDDDYVERVVEHLRTGRWAGVGGRKDGVGRTSQGRAIAAVMGSPFGQ
                     GNSVYHYGTETRTVDHIPFGAYPTALVRDLGGWNENQLVNEDFEFDYRVRRSGGELLF
                     DPAIRIDWDCRQSVSALFRQYRRYGAGKVQTLVRHPESVGLRHLAAPVVVGALAGSLP
                     LLAARRTRPLGAAALAGYGALVAVASMRTGTDLAPRDRRWVPAAFVALHLGWGLGFWE
                     EIAATAVGRGGPRRSLEAS"
     gene            complement(10900..12450)
                     /locus_tag="PLZ72_07325"
     CDS             complement(10900..12450)
                     /locus_tag="PLZ72_07325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA36140.1"
                     /translation="MLRYSDADELPATGSRLPRLRLVEPGVIDLTDPAPAIPSSVGLA
                     GPTTGPDASIIRNPPTSAIARRERQAIGRGLRLLGHVLDAAALAAPLVAYASLTDAVS
                     LKVTVLFVLVGTVLLWPTHRRGRHVVTPSEGLLSVITRIGLAPVVTSVLVTLSQFREL
                     SQPGKFIDVVAVLQIVLFTLPLVMLGRLITARLTATARNRGFDLDDTLIVGSGPVGQS
                     VASALVENPGFGLVPCGFVDRFDDQDDLPLVGDPEDLPEILQLTDVRHVVLAFGAASE
                     TELVRIVRRCPDGVQFYAVPRLFELGVSHENVGHEVDGLPLVPLRRPGTACHMWPAKR
                     AFDVITSGLLLLLTAPVMLACAVAVKLSSPGPVFFRQVRVGIGGRPFEILKFRTMKVN
                     DDSASQWSVDDDDRVTRAGRFLRPTHLDELPQLINVLRGEMSLVGPRPERPHFVEQFG
                     AEIEEYHERHRVPVGITGWAQVNGFWGDTSIETRVRLDNRYIENWSLWRDLVIALRTI
                     PTLLGKRR"
     gene            12793..13908
                     /locus_tag="PLZ72_07330"
     CDS             12793..13908
                     /locus_tag="PLZ72_07330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA36141.1"
                     /translation="MPSEPLHLEVVGELGALSTDWDDIVSLMERPSPFLRSWWLDGAG
                     AGTPAIVVVRRGGVLIGGAAFELDRLGRGPVSVERVRCLGQGPLAPDHLDVVAAPTDV
                     ADVLDAVLAWLRGSGPRVVDLDGLADDGALAGSLRDHVASRTAAPFASLTGGIDGYLA
                     ARPGKLRSTIGRTARRLEREGSTMTTVTGPATSADEADGVRAEAERALRDLHRLHDQR
                     WSDESGFLDAWDRFVAVATAGIERGEVRIHEVTSAAGEVVATELDLVLGDIVAFYQAG
                     RRTEREWRGAGTALRAWILRNAADSGAVEYDLLRGDEPYKADWSTGRRDVVRVRLGVG
                     RSGRVLHAAGSAWRRCAPHVHALARRLRRGGSGLNDP"
     gene            complement(13895..15388)
                     /locus_tag="PLZ72_07335"
     CDS             complement(13895..15388)
                     /locus_tag="PLZ72_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNA36142.1"
                     /translation="MGGPTSESAEPTDDPSSDEPSLGTTASRGFLWANLGIFTRYVTA
                     LILAAVLARVLSPVDYAVMVTLMLITFYFDNALDLGMGAALVYEQEEGVSERVQVAFT
                     ANVGLGAVLAALLIVVAPFVAGYFQQSAYVAEFRCLAVVVMLSAFTTVPWSLFMRNMD
                     FRRRAVVEVARDLSRFVATLGLALAGFGAWSVVIGLFVAYTVALVGTWLALRFRPTFA
                     WDLTTVKQLFRYAWRVAGNRVLGLLALNGDYFVVGNRRHDQYASYYQAFRLPEFVMGA
                     QLNGLSAVLFPMYSRIRSDGPEALRQGMYKALRIVALFSFPVGAGLALVARDAFTVFY
                     GTSNAAGIQTMEVISIAGAITGLGFATGDLLMAINRPGVLLRLNAVMVPLMLSSMWIV
                     SPYGIVWVATVHLVIQIVFVGARQLIVDHMVESPTSAALACLVPGLVVTAGIVAFALP
                     VRLLSDEGFLSLVAIGLAGTLGGLASLALYPPARHELTDLVAKLRGR"
     gene            complement(15420..16763)
                     /locus_tag="PLZ72_07340"
     CDS             complement(15420..16763)
                     /locus_tag="PLZ72_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36143.1"
                     /translation="MTPMRTSADLVGYTGEPAPVVERRPVLRLTRVQVMIVAISAVVT
                     AAVAAGFVSLQAPVYNTTRSVIVLSGSNPNDNDVLSRALESLASSKGMAAEVKRRGNL
                     DLSIDQIAGMISTRRSPESPYMDVVVSSPDQKLSEAVSAQVIPAMRGVFDQNQAEIPA
                     EQRIPGPIFQEVFAKPLQSTSTFPVWFAALFGALLGGLVPYLIFLFRNLRKPVVASAQ
                     DVTDAIDLPILVKVPVLAGRGGNAQDAVAGVISAVERLSLNEPIHRLVLVGADNGIDR
                     SALALALACVVARSFGQPVALVDADLERGTLTGMVNAEDVAGLSECLAGQLDPEKALL
                     TLSDAQIPRDLDGIAAPEGMVRFLGAGVDRSRNILRMRSTFHRVLEGMAGRYVVIING
                     PKVPGPVPTSQLLSLADATLMVVAEGQTSLTDARSAGDTLRSFSSGPAGVVVIRR"
     gene            16978..>17048
                     /locus_tag="PLZ72_07345"
     CDS             16978..>17048
                     /locus_tag="PLZ72_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HNA36144.1"
                     /translation="MGSLIKKRRKRMRKKKHKKLLRRT"
BASE COUNT         2451 a         5980 c         6222 g         2395 t
ORIGIN      
        1 cgagttcggc cagatccgcg cccttgcgca aggcggcgcg ggcgtcggcc acgccgccgg
       61 cggggacgac gaccgcggtc atgcagacct ggtcccagta ggcggggaca ccctcgtaga
      121 gccgccgcat gtccttgtcc gagctggggt cgatctcacc caggcgggcc tgcagcgcgg
      181 cccggtccac caggtagcgc agcaccgagt cccgggcccg gcccttcagc tcgggggcct
      241 gctggctgat ggtcgcctcg ccctgggcca tgtcctgctc ggagaccgtg gcgtcccaac
      301 gggcgacctc ctgggccagg acctcggagg agaccaggaa cgccagcacc tggcgggcgg
      361 cgggaccctc gaccacaccg gtgcgctggt cctgcagggc ctggttggtc atggccgcca
      421 tgaaggcgtc gtcggtggcc agctggttga ccagggttgc gctgatcgtg accccaccga
      481 cggtggccgc ggtgtcgtcc gcggccacac cgcagccgga caggccgacc gcggcggtca
      541 ggccgatggc ggtgatcaac aggaggcgcc gcggggatcg catggccgcg gatgctaccg
      601 agcggacccg tcgatcgttc aacgcgaccc ggggtcggtc cccacgatca tcgggccgcg
      661 tcgggcagca gctgggtcag cgcctcgacc aggtcggccg gcagcgtcgc cccggctcgc
      721 accggcagct ggagctgcga cgtctcctcc ttgtacaccg cgcccttgta caggcgggcc
      781 agccgcatct gcttggactg cgccagtcgc accggagcca ggcgcgccac ccaccccggc
      841 cccgccaggg cggaggcctt ggtgaccgcg atctcctcga ccccggtgcg cacgcactcg
      901 gcccgcagcc gtgcgatggc caggaggttg gccgccgggt cgggcacggg accgaaccgg
      961 tcctcccact cggcctggat gtcgtcgacg tcggcctggc tctgcacgcc ggccagccgc
     1021 cggtaggcgt cgaggcgcag gtcgtcgcgc tcgacgtagt cggcgggcag gtgcgcggcg
     1081 agcgggagct cgagggtgat ctcggtgggt tcgggcaccg gctcgccgtt gagctgggcg
     1141 atggcctcgt tgaccatctg cacgtacagg tcgtagccga ccgcggcgac atgtccggtc
     1201 tggccggttc cgagcaggtt gcccgccccc cggatctcca ggtcgcgcat cgcgatgcgg
     1261 aacccggatc ccagctcggt ggtctcgccg atcgtcttca gccgttcgta ggcctcctcg
     1321 gagagctgcc ggtccggggg gaagaacagg taggcgtagg cccgctggcc ggagcggccg
     1381 acgcggccgc ggatctggtg gagctggccg agacccagca ggtcggcccg ttcgaccacc
     1441 agggtgttga ccgtcggcat gtcgatgccg gactcgatga tcgtggtgca caccaggacg
     1501 tcgaactcgc cctcccagaa gtcgagcacg acccgctcga gggtgccctc gtccatctgg
     1561 ccgtgggcga ccgcgacccg cgcctcgggc accaggtcgc ggatgcgcgc cgcgtgctcc
     1621 tcgatgtcac gcacccggtt gtgcacgtag aagacctgcc cctcgcggag cagctcacgg
     1681 cggatcgcct cggcgacggc ccgctcgtcg tactcgccga cgtaggtgag gatcggctgg
     1741 cgggccgcgg gcggggtgtt gagcagcgac aggtcccgga tccccgtcag gctcatctcg
     1801 agggttcgcg ggatcggggt ggccgacagg gtgagcacgt cgacgtcgtt cttgaactgc
     1861 ttgatcgcct ccttgtggct caccccgaag cgctgctcct cgtcgacgac cagcagcccg
     1921 aggtccttga aggcgatgtc gccggacagg agccggtggg tgccgatgac cacgtcgacc
     1981 gatccgttgg ccagaccctc ggtgacctcg cgggcctggg cgttggtcag gaaccgcgac
     2041 aggacctcga cgcgaacggg gtagccggcg tagcgatcgg cgaaggtctg gaagtgctgc
     2101 tgggcgagca gggtggtcgg gaccaggacc gcggcctgct tgccgtcctg gaccgccttg
     2161 aacacggccc ggaccgccac ctcggtcttg ccgaagccga cgtcgccgca caccaggcgg
     2221 tccatgggga ccgtggactc catgtcctcc ttgacgtcgc ggattgcgct cagctggtcc
     2281 ggcgtctcct ggaaggggaa ggcggcctcc atctcgcgct gccagggcgt gtcgggcccc
     2341 gaggggtgac cggcggctgc ccgacggcgc tggtagagca ccaccagctc ctgggcgatc
     2401 tcggccaccg cggagcggac cttggccttg gtgcgggcga agtccgcccc gcccatgcgg
     2461 ttgagcgacg gggtctcgcc gccggagtag agccggatgg cgtcgatctg ctccgaaggg
     2521 acgtagagct tgtcgtcgcc ccggtactcg agcaggaggt agtcgcgggc ggcaccgccc
     2581 atggtgcgtt cgaccatgcc ggcgaagcgg gcgacgccgt ggtgctggtg cacgacgtag
     2641 tcgccgacct tcaggtcctc gtagaacctc tgggcgtcac gacggggcgc cttgggcggc
     2701 cggtgggtgc gccgacggcc ggtgaggtcg gcctcggcca gcagggccac cttcaccccg
     2761 ggcaggatgc agccgcggtc gatcggggcg acgacgaccg tgccgcccgg ggcgagcagc
     2821 gcccgggcgt cgacctcgcc gtcggttccc ggctcgagca cggcgaagct caatccgctc
     2881 tcggcgatca gctgggccag gcgtgtggcg gagccggacc cgtccgcgca caccacgacc
     2941 cggtagccgt cggccacgag ctggcgcagc tggccgatca gcgcctcgcc cccgccgacc
     3001 gcgggcggcc accccgagga cgcgaccgca ggggtgtcgg gcgagtcggg caccgcggcc
     3061 accgaccagg tgggggcctc ggtgcgcgcg agcaggcgct ccatgtcgag gtgcagccgg
     3121 ggcaggtcga cccggtcgtc gaggccccag gtgcgagcga gtgtggcgcc caggtccgcc
     3181 tcctcggcca gcacgtcgcc gacccggtcg cgcagacgac gtggttcgac cagcaggacc
     3241 agcccgtcgt cgtcgaggag gtcgaccagc acgtgctcgg gtccgccgtc gtggtcgacc
     3301 agccacggca accaggcctc catgccctcg aacacctcgc cgtcggccag gcgctgccac
     3361 tgctcgcggc cccaggggtc ggtggccagg agccgttcgg cccgctcgcg gaccgcggcg
     3421 gtgggcagga gctcccgcgc cgggtacagc tcgacgtggg tcaggtcggc gccgctgcgc
     3481 tggtcggcga cggagaactc ggccaggcgg tcgacctcgt cgccccacag gtcgatgcgc
     3541 acgggccggt ccgcggtcga cgggaagacg tcgatgatcg atccgcgcac cgccacctcg
     3601 ccgcggtgtt cgacctggta ctcgcgtcgg tagcccgaca gcaccagccg ctcgaccagc
     3661 tcctgctggt cgaggcgctc cccgggcacg atccggacgg gctcggtgtc ctcgacgccg
     3721 gggccgagcc gctgcgccag cgcccgggcc ggagcgacca ccacctggag ggtgtcgtca
     3781 ccgcagcgca gccgacccat gacccgcagc cgacggccca tcgtctcgat cgacgggctg
     3841 acccgttcga acgggagcgt ctcccaggac gggaacagct cgacggcgtc ctggggcagg
     3901 aacgcggtca ggtcaccggc cagtcgttcg gcttcggcca cgttcggcac cgcgacgacg
     3961 atcggtcggc ggcgcgacag tgcggtgagc ccggccaggg ccgcagcccg ggccgcgtcc
     4021 ggcagcacca gcaccgtgga gcgacgcccg agggcaccga ggagaccggg ctcgtcgcgc
     4081 aacacgccga gcaggcccgc cagcggcggt ccggtcgggg ctgcggtcgg ggcgggttcg
     4141 tcgtccatgg gctgctggaa cctacccgcg gtgtgggagc atcggcgccc gtggccgtcg
     4201 tcctcgcaac cctctccgcg atcgcgttcg gcgtcggcga cttcctgggc gggctgtcgg
     4261 cccggcgcat ggcggcggtt gccaccgccc tgggcaccca ggtcgtgggc ctggtcctga
     4321 tcctcgccct gtccccggtc atcggcggca ccgtgaccgc gaccgacctc ggatggggga
     4381 tggcggccgg ggtggtcgga tccagcgcac tgatggtctt ctactgggcg ctgggcgccg
     4441 ggcagatgag cgtcgtcgcc ccgatctcgg cggtgacctc ggccctggtc ccgctctgct
     4501 tcggcctcct ggacggagag cgaccgggtg cggtcgccat gacgggagcc ctgctggccc
     4561 tgccggccat cgtcctcatc tcccgcgagc cctccgatcc ggtcggcgcc gacgaacgcg
     4621 acgatccacc ccatcgggca cccggttccc gggtggtggc ggcatcggcc gtcgccggcg
     4681 cgggcttcgg ggcgttcttc gtgctgatct cccgctccgg cgaggacagc gggctgtggc
     4741 cgttggcgtc ggcccggtcc gtggccgtcg tcgtggtcgg tgccgcggtc ctgctgaccg
     4801 gggtgcggcg ggtcgagcgc accggcgtgc agctggcgat cggggcgggg agcttcgacg
     4861 tggccgcgaa cgcgctgttc ctgctggcca gccgtcaggg gttgctcacg ctcgtcgggg
     4921 tgatcggttc gatgtacccg gcctccacga tcgtcctggc ccgggtggtg ctgcgcgaac
     4981 gcatcgcccg ccaccagatg acgggcctcg cactcgccgc cgcgtcgctc gtcgcggtca
     5041 cgatcggctg atccgaccgt cggggttcag ccgcgttggt tccagcgctg catcgcggcg
     5101 tcgagcccgt cggtggcgat gtcggcgacg gcgtccgcgg cctcggcgat catgacgtcg
     5161 agctcgtcgc ggacccgccc ccggaccttg gacagcaccc agtcggcacc gcggtccgag
     5221 cctccgggcg gcttgcccac ccccatccgc acccggacga agtcggcgga gtggaggtgc
     5281 gcgtcgagcg agcgcagtcc gttgtggccg gccagtcccc cgccgagctt gagcttgagc
     5341 cgacccggat ccaggtcgag ctcgtcgtgg acgacgacca ggtgagccat gtcctcgatg
     5401 ccgtagcgac ggaccagcgc cacggcggcc tgcccggact cgttcatgta ggtggtggga
     5461 acggccagca ccacccggat gtcgccgagt cggatgtcgg cgacgcgggc gttgacccga
     5521 ccgtcgacct tcaaccgggt gccgtgacgc cgggccagca gctcgaccgc ctcggccccc
     5581 acgttgtggc gggtcccggc gtagcggtca ccggggttcc cgagtccgag gaccagcagg
     5641 tcccagggca tcagccctcg gcgtcgccgt cgccggcggc ctcggcgtcg ccgtcgccgt
     5701 cgccgccctc ggagtcgtca ccctcgttgc gggcggtgac cgcggcacgg gtgagctccg
     5761 cggtcacgac cgcctgctcg gggtcggtga ccagctcgac gccggccggc agcaccaggt
     5821 ccgccgcggt gatggtcgtg gtgagcgtga ggtcggtgat gtcggcgtcg atcccgtcgg
     5881 ggatggcacc cggcttcacc aggaccttca gggccatcag ccgctgctcg gtgatgccgc
     5941 cgttctggga gacctccttg gcctcgccca ccaggtggat cggaacctcg acctcgatcg
     6001 cggcgtccgg atcgacggtc aggaagtcga cgtgggtcac ctcgcggcgg accgggtgac
     6061 gctggagctc cttgacgacg gtcaggtagg tcgtcccgtc gacggtcagc gagatcaggg
     6121 cgttctggcc ggcgtcggtg ctcagcgccc gacggagttc cttgcggtcg accgacagga
     6181 cggtcgcgtc cttgccctcg ccgtagacca caccggggat gaggccttcg cgacggaggc
     6241 gacgggacgg acgggtcccg gtctcccggc cggtggaggc ggtgagcgtg acctcggaca
     6301 tgacggaact cctgcgaaac tctgcggacg gccaccccgt cgggtgacgg cggactcggc
     6361 ccgaacgggc gacccgccac gttaccggcc gggcgcaccg tcgggccagc caccgcccgc
     6421 cgtgaccgga gacgaatgtg agcaggaccg acctgaaccg acaccgaccg cgaagggcgg
     6481 ctgtcgtgct cgtgtcgacg gtgctcgccg tcgccgtcgg agcgtgtgcc ggcaaccgcg
     6541 accggtcgtc ccgtgcgtcg accacgacga ccgcacggac cacttcgttc gtgaccgccg
     6601 gcgagcggct gggcgtcgga ggctgtccgc tcttcccccg ggaccacgcg ttccacgcct
     6661 ccgtcgccgc ccttcccgtc cgagcggact ccgccgaggt ggtcgcggcc ctggggggtc
     6721 ggtccctggc ggtccgcccc gccttctcgg cctcggtctg gcaggggtcg cgttcgggca
     6781 tcccggtcaa cgtcgtcgac tcacgactca ccgcgcccgt cgacgtcgat ggtgggacct
     6841 acgcctacct ctcggacctc tccggtcatc cgatccccgc ctcaccacgc ctggagggat
     6901 ggccggggat ggcctgggac cgccacctgc tgatcgtcga ctccgcgacc tgcaccgcgt
     6961 cggagatgtt cttcgtcacc ccgcccggac tcagcccgac ccgccgctgg ggcgccgaga
     7021 cggcggttcg gatcgacctc gggtcgaact ctccgcgagc cgagggcacc gccaccgcgt
     7081 cgggcacgtc gatgctcgcg ggcatggtcc gctacgacga agtggcccgg gaccgactcg
     7141 atcacgcgat cggagtgtcg gtcccgacca tccgtgccgg agcggtccgc tggcccgcca
     7201 cccacaccga cgggcgctcg gaggaccccc gagccccgga gatgggcagt tggttgcggc
     7261 tccgacgcga cgtggacctg tcagcgttgg gtccccaggc ccgggtcgtc gcccgagcgc
     7321 tgcgggacca cggtgcggtg gtggtcgaca cgaaccacga cggcctcgcg ctctcgggag
     7381 aacccgacga gcggtgggac gatgccgacc tcgccacgct ccgcaccctg acggcagccg
     7441 acttcgaggt ggtcgatccc gacccgatgc gggccgaacc gggcacggag tcgtaccgga
     7501 tccgctgaga acatccgacc ggggtgcggc cgacctcagt gctggttctg gcccccgaag
     7561 atctcgctga ccgacgtgtc ctcgaacacc gcgtcgatgg catcggcgat gatcccggcc
     7621 gcggagagga tctcgatctt gtcgatgtgc ttctcgggtg ggagcggcag ggtgttggtc
     7681 accacgacct tctccaggtt cgagttcttc aaccggtcga tggcgggccc ggagaacacg
     7741 ccgtgggtcg ccgcgcagta caccgacgag gcaccgtgct ccgccaactg ctcggcagcc
     7801 gcgacgatcg tgccggcggt gtcgatcatg tcgtcgatca gcacgcaggt ccggccctcg
     7861 acctcgccga cgacgtcctt ggcctcgacc tggttcttgg cacccttcac ccggcgcttg
     7921 tgcacgatgg ccaggtccgc gtgcagggcg ttggcgtagc gctcggccac cttcacccga
     7981 ccggcgtcgg gcgacacgac caccaggtcc tcacccaggt tgtcggccat ccactgcacc
     8041 agcaccggca tggcggtcag gtggtcgacc ggtccgtcga agaagccctg gatctgaccc
     8101 gagtggaggt ccaccgacac gatgcgcttg gcgccggcga cctcgaacat gttggcgagg
     8161 agcttggcgg tgatcggctc gcgtccctcg gccttgcggt cctgacgccc gtacccgtag
     8221 aagggcgcga cgatcgtgat ccgcttggcc gacgcccgac gggcggcgtc gaccatgatc
     8281 agctgctcca tcagcgcgtc gttgaccgac agctcggagg tggagcagtg gctctggaag
     8341 ataaagacgt ccgctccacg gatcgattcg ccgaacttgc agtgcagctc gccgttggcg
     8401 aactcggcga tctccatggg ggccagcgag atgccgaggc gctcggcgat ctcctccgcc
     8461 agctcgcggt tcacgcgacc ggccaccatc gtcagccgct tcttggtgac cagttccatg
     8521 ccccggacct cgttccccgc gtcgtcgttc aggtggcggt gtccgcagtg tagaacggcc
     8581 cggtcgtgac atccgacgcc aactcggcgc ccggaccaag cgacgcgaac gggccgacgg
     8641 tgcagcggtc accgatgcgg gccgagcgcc cggtgctctg ctcgatcacg cagtcgtctc
     8701 cgacgatgca gtcgaccaag cgggtgttgg gacccagctc ggtgccgtcg ccgaccaccg
     8761 tggcgccctg gaggatcacc ccggggaaca gcgtgacgtc ctggccgacg cgggcggtcg
     8821 tgtcgacgaa caccgacgca ggatcgagca tggtgacccc ggagcgcagc agcgcctcgt
     8881 tggtccgacg gcgcagctcg gcctccgcag cggcgagctg gacccggtcg ttgaccccct
     8941 gggtctcggc tgcgtcaggg gccacgacgg cctctacccg gtgaccggtg cccgacagga
     9001 ccgacaccac gtcggtcagg tagtactcgc cctgggcgtt gtcgggctgc acccgccgca
     9061 gcgcgggggc gagcaggttg cgacggaagc agtagatcga cgtgttgatc tcgtccaccg
     9121 ctcgttcctc agcgtcggca tcggtgtgtt cgaccacacg ttcgacgctg ccgtccccgg
     9181 agcggatgac ccggccgtag ccggtcggat cgtccatcac cgcggtgagc agggttgcgg
     9241 cagcacccgt gcggcgatgg tggtcgacca acgacgcgat ggtctcgggt cgcagcagcg
     9301 gggcatcgcc gggcaggacc aggacgtcac cgtcgtcatc gtcgtcgggc agcccgacca
     9361 gtccgaccag cgccgcgtcg ccggtcccgc gctgcacccg ctgctcgacg aagtccaccg
     9421 cgacgtcacc cacgtgctcg agcatggtct tggccacccg gtcgcccttg tggccgacga
     9481 cgatcgccac ccgcgaggtg tcggtgccgg cgagcgagtc gatcacgtac atgagcatcg
     9541 ggcgtccgca caggcggtgc agcggcttgg ggcgcgacga ctgcatccgg cggccctcgc
     9601 ccgcggcgag caccaccgcg gcgagcggcg ggggcggtcc ggcggcggtg tcgatcgggg
     9661 tctcgttcat gggtcctcgg gagtcggacg ggccggtggg gatggctggc ggggcaggat
     9721 tcgaacctgc atcttcctga tccaaagtca ggcgttctgc cgttgaacta cccgccaccg
     9781 gttcggggat caacgtagtg cgccgcggtc tcggtcctcc caccgcgcgg cgacggggtc
     9841 ctcaggacgc ctccagggag cgtcgcggac cccctcgccc gacggcggtc gctgcgatct
     9901 cctcccagaa gccgagtccc cacccgaggt ggagcgcgac gaaggcggca ggaacccacc
     9961 gtcggtcccg gggtgccagg tcggtgccgg tgcgcatcga ggcgaccgct accagcgccc
    10021 cgtagccggc cagcgccgcg gcgccgaggg gtcgggtccg ccgagcggcg agcagcggga
    10081 gcgatccggc cagggcgccg accaccaccg gggcggcgag gtggcgcaac cccaccgact
    10141 cggggtgacg gaccagggtc tggaccttcc ccgccccgta gcgacggtac tggcggaaca
    10201 gcgccgagac gctctggcgg cagtcccagt cgatgcggat cgccgggtcg aagaggagct
    10261 ctccgcccga tcggcgcacc cggtagtcga actcgaagtc ctcgttgacc agctggttct
    10321 cgttccagcc ccccaggtcc cggacgagcg cggtcgggta ggccccgaac gggatgtggt
    10381 cgacggtccg ggtctcggtt ccgtagtggt acaccgagtt gccctgaccg aacggcgagc
    10441 ccatgaccgc ggcgatggcc cggccctggg aggttcgacc caccccgtcc ttgcggccgc
    10501 cgactccggc ccaccgaccg gtccggagat gttcgacgac ccgctcgacg tagtcgtcgt
    10561 cgatccggca gtgggcgtcg acgcgcacga accactcgcc gcgggcgtgg gccagcgcca
    10621 ggttcatggc ccgggggatc acccggtcgg ggttggccac cagctcgacg cggggatccg
    10681 agcgggccac gtcggcgacg atcgccgcgg tgtcgtcctc ggaggagccg tcgaccacca
    10741 cgatctgcag gtcggggtag gtctgggacc gcacggcgtc gagcacgcgc cggatggagg
    10801 cggactcgtt gcgggcgggg atggcgacgg tgaccagacc gggcaccgtc tcggacgccg
    10861 ggatggcgac ctcgggtgat ccggcgcgtg cgccggatgt caccgacgct tgccgagcag
    10921 ggtcgggatc gtccgcagtg cgatcaccag gtcccgccac agcgaccagt tctcgatgta
    10981 gcggttgtcg agccggaccc gggtctcgat gctggtgtcg ccccagaacc cgttgacctg
    11041 ggcccacccg gtgatgccga ccgggacccg gtggcgctcg tggtactcct cgatctctgc
    11101 gccgaactgc tcgacgaagt gcgggcgttc cggccgtgga ccgacgagcg acatctcacc
    11161 gcgcagcacg ttgatcagct ggggaagctc gtcgagatgg gtgggacgca ggaaccgtcc
    11221 ggcgcgggtg acccggtcgt cgtcgtcgac gctccactgg ctggccgagt cgtcgttgac
    11281 cttcatggtc cggaacttga ggatctcgaa gggacggccg ccgatgccga cgcgcacctg
    11341 gcggaagaag accgggcccg ggctggagag cttgaccgcc accgcgcacg ccagcatcac
    11401 cggggcggtc agcagcagga ggagtcccga ggtgatgacg tcgaaggccc gcttggccgg
    11461 ccacatgtgg cacgccgtgc cgggacgccg caggggaacc agcgggagtc cgtcgacctc
    11521 gtgaccgacg ttctcgtggc tgaccccgag ctcgaagagc cgagggacgg cgtagaactg
    11581 cacgccgtcg gggcaacgac gcacgatccg gacgagctcg gtctccgatg cggcaccgaa
    11641 ggcgaggacg acgtggcgga cgtcggtgag ctgcaggatc tcggggaggt cctcgggatc
    11701 gcccaccagc ggcaggtcgt cctggtcgtc gaagcggtcg acgaacccgc aggggacgag
    11761 cccgaagccg gggttctcga ccagggccga ggcgacggac tgaccgaccg gacccgagcc
    11821 gacgatgagg gtgtcgtcga ggtcgaagcc ccggttgcgg gcggtggccg tgagccgggc
    11881 ggtgatgagg cggccgagca tgaccagcgg cagggtgaac aggacgatct gcaacaccgc
    11941 gaccacgtcg atgaacttgc ccggttggga cagctcgcgg aactgcgaca gcgtcaccag
    12001 gaccgaggtg accacggggg ccagtccgat ccgggtgatc accgacagga ggccctcgct
    12061 gggggtgacc acgtggcgac cacgacggtg ggtgggccac agcagcacgg tgccgaccag
    12121 cacgaacagg acggtcacct tgagcgacac ggcatcggtg agcgaggcgt aggcgacgag
    12181 cggggcggca agggccgccg cgtcgaggac gtgaccgagg agccggagac cccgaccgat
    12241 ggcctggcgc tcccgacggg cgatcgccga ggtcggcggg ttgcggatga tcgaggcgtc
    12301 gggcccggtg gtcggccccg ccaggcccac gctcgacggg atcgcgggcg ccgggtcggt
    12361 caggtcgatg acacccggtt cgacgagccg cagtcgcggc aggcgcgacc cggttgccgg
    12421 tagctcgtcc gcatcggaat acctcaacat atcgcccctc ttccccagct ccggacgtgt
    12481 ccgtccgata ccgaagtggg ttgtcggttc cccccgactc acccgaccca gctcgtcttc
    12541 tgctgctcca gccgaagccg cagccctgca ctgaccctga ctctgacccg ggccgaatcg
    12601 ccgatcctcc cccgagtgac cgacgggccc ggttccccgt cccggcacaa tgtcagcatg
    12661 tgacgaccgc caccgtcaag ggtcaacccc ggggcaaatc ccccggaagg gtcggcgaca
    12721 tgggtcacac ggcccccaca cgcaccgccg tcccgatggc gcggggttgt cgccgtccgc
    12781 cacactcccc cgatgccgtc ggagccgttg cacctggagg tggtgggcga actgggtgcc
    12841 ctgtcgaccg actgggacga catcgtgtcg ctcatggagc ggcccagccc gttcctgcgg
    12901 agctggtggc tggacggagc tggggcgggc actcccgcca tcgtcgtggt gcgccgcggg
    12961 ggcgttctga tcggcggcgc tgcgttcgag ctggaccgcc tcggccgagg accggtctcg
    13021 gtcgaacggg tgcgatgcct ggggcagggg cccctcgccc cggaccacct cgacgtggtg
    13081 gccgcgccga ccgatgtggc cgacgtcctc gacgcggtgc tggcctggct gcgcgggagc
    13141 ggaccgcgcg tcgtggacct cgatggcctc gccgatgacg gagcgctggc cgggagcctg
    13201 cgcgatcacg tggcgagccg caccgccgca ccgttcgcgt cgctcaccgg cggcatcgac
    13261 gggtacctgg ccgcccggcc cgggaagctg cggtcgacga tcggtcggac ggcccgtcgc
    13321 ctcgagcggg aagggtcgac catgacgacg gtgaccggtc cggcgacgag cgccgacgaa
    13381 gccgacggag tgcgggccga ggcggagcgg gcgctgcggg acctgcaccg gctccacgac
    13441 caacgctggt ccgacgagtc ggggttcctc gacgcctggg accgattcgt cgcggtcgcc
    13501 accgcgggca tcgagcgcgg cgaggtccgg atccacgagg tgacgtccgc cgcaggagag
    13561 gtcgtggcaa ccgagctcga cctggtcctc ggcgacatcg tcgccttcta ccaggccggc
    13621 cgacgcaccg agcgggagtg gcgcggagcg ggcaccgcgc tgcgggcgtg gatcctgcgc
    13681 aacgccgccg actccggtgc cgtggagtac gacctgctcc gcggtgacga gccctacaag
    13741 gccgactggt cgaccggacg acgcgacgtc gtccgggtcc gcctcggcgt cggccgatcc
    13801 gggagggtcc tgcacgcggc ggggtcggcg tggcggcggt gcgccccgca cgtccacgcg
    13861 ctggcacgac ggctgcgccg gggaggctcg gggctcaacg acccctgagc ttggcgacca
    13921 ggtcggtgag ctcgtgtcgc gccggcgggt acagcgccag ggaggccagc ccaccgaggg
    13981 tcccggccag cccgatcgcg accagcgaca ggaacccctc gtcgctcagc aggcggaccg
    14041 gcagggcgaa ggcgacgatc cccgcggtga cgacaaggcc ggggaccaga catgccaggg
    14101 cggccgacgt gggcgactcc accatgtggt cgacgatcag ctggcgggcc ccgacgaaca
    14161 cgatctggat cacgaggtgc accgtggcca cccacacgat cccgtagggc gacacgatcc
    14221 acatcgagct cagcatgaga gggaccatca ccgcgttgag gcgcaggagg acgcccggtc
    14281 ggttgatcgc catcaggagg tcgccggtgg cgaagcccag cccggtgatc gccccggcga
    14341 tcgagatgac ctccatggtc tggatcccgg cggcgttgga cgtcccgtag aagacggtga
    14401 aggcgtcacg ggcgacgagc gcgagtccgg cccccaccgg gaacgagaac agcgcgacga
    14461 tccgcagcgc cttgtacatg ccctgtcgca acgcctccgg tccgtcggag cggatgcgcg
    14521 agtacatcgg gaacagcacc gcggaaaggc cgttgagctg cgctcccatg acgaactccg
    14581 gcaaccggaa cgcctggtag tagctggcgt actggtcgtg acggcggttg ccgaccacga
    14641 agtagtcgcc gttgagggcc aacagcccga ggacccggtt gcccgcgacg cgccaggcgt
    14701 agcggaacag ctgcttcacc gtggtgaggt cccacgcgaa cgtcgggcgg aaccgcaggg
    14761 cgagccaggt ccccacgagg gcgacggtgt aggcgacgaa cagcccgatg accaccgacc
    14821 acgcgccgaa gccggccagg gcgaggccca gggtggcgac gaagcggctc aggtcacggg
    14881 ccacctccac caccgcccga cgacggaagt ccatgttccg catgaacagc gaccacggca
    14941 ccgtcgtgaa cgcggacagc atgaccacga cggccaggca gcggaactcg gccacgtacg
    15001 ccgactgctg gaagtagccg gcgacgaacg gggcgacgac gatcagcagc gccgcgagga
    15061 cggcaccgag gccgacgttg gcggtgaacg cgacctgcac ccgctccgag acgccctcct
    15121 cctgctcgta gacgagagcg gcgcccatgc ccaggtcgag ggcattgtcg aagtagaagg
    15181 tgatgagcat cagcgtgacc atcaccgcgt agtcgacggg gctgaggacc cgggccagca
    15241 ccgcggccag gatcagcgcg gtgacgtagc gggtgaagat gccgaggttc gcccacagga
    15301 acccccggct ggcggtcgtg ccgagcgacg gctcatcgga tgacggatcg tcggtcggct
    15361 ccgcgctctc ggacgtcggt ccacccatgg tgctcaggcc ggttccgggt ccgcggcgct
    15421 cagcgccgga tgacgacgac gccggcggga ccggagctga aggaccggag ggtgtcgccg
    15481 gcggagcggg cgtcggtcag gctggtctgc ccctctgcga ccaccatcag cgtcgcgtcg
    15541 gccagcgaca gcagctgcga cgtcggcacc ggtcccggga ccttggggcc gttgatgatg
    15601 accacgtagc ggccggccat gccctcgagc acccggtgga acgtggagcg catgcgcagg
    15661 atgttgcggc tgcggtcgac ccccgcgccg aggaagcgca ccatcccctc cggcgcggcg
    15721 atgccgtcga ggtcacgggg gatctgggcg tccgagagcg tgagcagcgc cttctcgggg
    15781 tcgagctggc ccgccaggca ctccgagagc cccgccacgt cctcggcgtt gaccatcccc
    15841 gtgagggtcc cgcgctcgag atcggcgtcg accagcgcga ccggctggcc gaagctgcgg
    15901 gccaccacgc acgccaacgc cagcgccaac gccgaacggt cgatgccgtt gtcggcgccg
    15961 accagcacga ggcggtggat cggctcgttc aggctcagcc gctccaccgc ggagatcacc
    16021 ccggcgacgg cgtcctgggc gttgccgccc cggccggcca ggaccgggac cttgaccagg
    16081 atcggcagat cgatcgcgtc ggtgacgtcc tgggccgagg cgacgaccgg cttgcggagg
    16141 ttgcggaaca ggaagatcag gtacggcacc aggccgccga gcagcgcccc gaacagggcg
    16201 gcgaaccaga ccgggaacgt ggaggtggac tgcagcggct tggcgaacac ctcctggaag
    16261 atcggtcccg ggatccgctg ctcggcgggg atctcggcct ggttctggtc gaacacgccc
    16321 cgcatggcgg ggatgacctg ggcgctgacc gcctcggaca gcttctggtc ggggctggag
    16381 accaccacgt ccatgtaggg cgactcgggg gaccgacggg tggagatcat cccggcgatc
    16441 tggtcgatgc tcagatcgag gttcccgcga cgcttcacct cggcggccat gcccttggag
    16501 ctggccaggc tctcgagcgc acgggagagc acgtcgttgt cgttggggtt ggagcccgac
    16561 agcacgatca ccgaccgcgt cgtgttgtag accggcgcct gcagggacac gaagccggcc
    16621 gcgaccgcgg cggtcaccac ggcgcttatc gccacgatca tcacctgcac ccgggtcaga
    16681 cggagcacgg gacgacgctc gaccacgggg gccggttcgc cggtgtagcc gaccaggtcg
    16741 gccgacgttc gcatgggtgt catcggcgcc agcgtctcca tggtccgggc cacccgggag
    16801 gttccgggcg gcgcggggcg tctcgtcggg agctcgacgg gcatcggtgc ccgcggagcc
    16861 actccggttc gggagtcgag tgtaccgagc gggtcggccg gaccggaatc ggtggatccc
    16921 ccagcgcgac cgccggtttg ggttcgtcac gccgggccgc ctactgtgtc cgccgccatg
    16981 ggttccctga tcaagaagcg ccgtaagcgc atgcgcaaga agaagcacaa gaagctgctg
    17041 cggcggac
//
LOCUS       DAOQOD010000056        17033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1191_length_17033_cov_11.947933, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000056 DAOQOD010000000
VERSION     DAOQOD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 17033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17033
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_17033_cov_11.947933"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLZ72_07350"
     CDS             <1..1068
                     /locus_tag="PLZ72_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA36145.1"
                     /translation="RVRIVTPDGRRLSLGTHASKRAAEAAYARSVTDQADGKLATPSA
                     VTTPTLGDYASSWVETRLTKRGEPLRPRVKDLYLMQLRLHIVPTFGTRRLARITTAQV
                     RGWNARLRGPDGPGASTAAKCYRLLRSILTTAVEDGLIPANPCTIKGAGVEPADERPI
                     PTVAQVHDLVEHLPERMRCVALLAAFVGLRRGEILGMRLGDVDLDRREIAIVRQRQLD
                     RHGNHLVCPPKSDAGRRVLTIPAALAVDLRGHLDAFAQPGDDGYVFTGQKGGPLAPHV
                     WQDAWEKARTEVGIPEIHLHDLRHLAGTLAASTGASTKEVMYRLGHGTHQAALRYQHA
                     TRHRDRAIADALDELIAPRSN"
     gene            complement(1127..1561)
                     /locus_tag="PLZ72_07355"
     CDS             complement(1127..1561)
                     /locus_tag="PLZ72_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36146.1"
                     /translation="MPTTAVRRIAFAALIAAASIGPIAACSDDSDDAADTDSTTTTAE
                     ATATASATDDLDAFCAAATELVGVTPTSEQLAEYAQLAPQDIAAPVETFIAAFESADG
                     DLGAVFADPEASAAADEIAAFESEACGITQPGPPPGAPGTEG"
     gene            complement(1638..2291)
                     /locus_tag="PLZ72_07360"
     CDS             complement(1638..2291)
                     /locus_tag="PLZ72_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36147.1"
                     /translation="MNDGPGHLGGTLRYLVAATHWSLLAGSGLIIGAGVALVARWPEA
                     LWPVHGTALGLMVGMAAVAVDERCATVVDASPRPLWWRTAVRSAGPLALVLFWATAHW
                     ALRAPLPDRLDVLILQGAVACGIGFGLATAARASGRSEPGVTLAAAAVPLVAGAALAR
                     PFEPDLPLFPVWPHEDWDRALLIWTVLGTAAALVAARALWRDARPRRNPGVTHRPDR"
     gene            complement(2288..3196)
                     /locus_tag="PLZ72_07365"
     CDS             complement(2288..3196)
                     /locus_tag="PLZ72_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36148.1"
                     /translation="MTAAESPAIVTVAEPARPPWMDLARIEVRRYAGRPAFLVGLVLT
                     YATLLPYLDGDEPTDELSMIAPAALLGMVGLSVSAHRVWASDRCAEAAGPTPVGQASR
                     TVGHLAACVVPFLAGLGFVLLTVLRAQSVPTVRGGYAEMMSDGWVAAVWFALGAVSCL
                     GGPILGVVVARKTKRAAAPIVTTVGLVLAVIVFQGLFEPLRRVRVVLPWTYWGGPFGI
                     DGDPQRSVVLSGSPGWWLTYLIVLCAIGAVVAVRTDVEARSARGRTALIVLAGLAVVT
                     VVLAMFTGTAETLVSPVPGDGPAWGG"
     gene            complement(3193..4059)
                     /locus_tag="PLZ72_07370"
     CDS             complement(3193..4059)
                     /locus_tag="PLZ72_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36149.1"
                     /translation="MPTTVRVSHVTRRYGPVAVLDDVSFDLSPGITALLGPNGAGKTT
                     LLGLLSTASRADAGEITVLGRRADGPLAERTEIRRRLGFLPQEVRFPAGMTAFGFLDY
                     IAVLKKWDDTAARHDEVRRVLGVVGLEDRPTIKVRKLSGGQRRRLAFAQALIGDPDLL
                     ILDEPTTGLDPEQRATFRSTLSERARHGTVLLATHQTEDVAAVCDRVIVLVGGRVAHD
                     GDVAGFVASARDRVWLGERPVADAVATWRDPGGGVRSVGGTPPADAQAAPPTVEDAYL
                     LLVGPRAVQGAA"
     gene            complement(4071..4925)
                     /locus_tag="PLZ72_07375"
     CDS             complement(4071..4925)
                     /locus_tag="PLZ72_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36150.1"
                     /translation="MSAWHVDDTVATAYGTRRLDAVQRASVEAHAVRCEDCRAFLAAS
                     ATGDHRRTDLDLDLDQMWTRIEAAISAPELGRGGQALLRLGASMSDVVVTRVVAAQSR
                     QWTIATTLVLAVTAVAAVLGPVDSAHAAFLIVAPLLPALGVAATYRLVPHGIHLLERA
                     APFSPARMLLWRTAYVVFTAVPVAVAVGAVVLHHPGAAVSWLLPALACTLCVTAASTW
                     SDPVRPAAFVALGWTGVIIAWQVRDTPWAITTASTQLVAVAVSVGAALLLHRRLTSDP
                     APSAIGGT"
     gene            complement(4922..5494)
                     /locus_tag="PLZ72_07380"
     CDS             complement(4922..5494)
                     /locus_tag="PLZ72_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA36151.1"
                     /translation="MAGGSGDERRDVVLVAAIASGDAAALRVLYDRHAPWLVARLRAR
                     CWDDALVADAVQDTFLAVWRGAGRWRADGDVGAWIWGIGVRQLVTHLRRAGRQARPAP
                     FPAQGAEASTEERVLESIDLGEAGAVLASLSPELMEVVQAMVFDGLTAKETGRMLGVP
                     EGTVKGRMRKAKSVLRTELAGTSLQLGDLR"
     gene            complement(5687..5760)
                     /locus_tag="PLZ72_07385"
     tRNA            complement(5687..5760)
                     /locus_tag="PLZ72_07385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5726..5728),aa:Gly,seq:ccc)
     gene            5890..6456
                     /gene="dcd"
                     /locus_tag="PLZ72_07390"
     CDS             5890..6456
                     /gene="dcd"
                     /locus_tag="PLZ72_07390"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNA36152.1"
                     /translation="MILCDRTIRAEIESGRIVVDPFDPAMVQPSSIDVRLDHRFLVFR
                     SHTRPVIDVKQDLSDLTELVEADDDRPFILHPGEFVLGSTAERVGIPDDLVARIEGKS
                     SLGRLGLLIHTTAGFVDAGFEGHITLELSNVATLPITLYPGMKVGQISFIRMDGPAEH
                     PYGTGALGSKYSGQVGPTPSQYWKNFDA"
     gene            complement(6527..6880)
                     /locus_tag="PLZ72_07395"
     CDS             complement(6527..6880)
                     /locus_tag="PLZ72_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36153.1"
                     /translation="MTLGHVVVVVRPGDPGGVMLGDLLGAAGAEVLLTDSVALAAGTA
                     LSDPPSTIVLATPATTLVADLRGSLSARQRGVSVVVVVDDADAADSALADGADSVLIR
                     PVEAERLVEAIDRLS"
     gene            complement(6891..9167)
                     /locus_tag="PLZ72_07400"
     CDS             complement(6891..9167)
                     /locus_tag="PLZ72_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNA36154.1"
                     /translation="MSATDTSSDATHDSDAPTPVPGPKRLQPHQISVAIGIVIALIVV
                     VSGIAASTLQWHDDSPIQREVFGGVPGALKFAFYVVIPIMFIIGSVMFANRVRNWERG
                     GPDRRTITPKNAKKRMEKLRAGLYMQTLLRDPAAGIMHSMIYFGFLVLLAVTSILEIN
                     HQLPEGIKFLHGGVYEAFSFIGDLGGVVFLTGVTWAIVRRYIQRPYRIRIKTKPEHAV
                     ILGVLAAIGVTGFLAEGFRIADTGMPSFEKWSFIGYPLALVFENMSNLDTWHQVMWVA
                     HILTFVAFLVILPVTMLRHIFTSPLNMYLSEKDRPKGAMKAMPNLMETELESFGAYRV
                     EDFTWKQLLDTDACTMCGRCTAVCPAHATGKPLDPREIVLKTGEVMAATGNPVVSPPI
                     GVDSEITVSADLLFERITTEEIWSCTSCKACDENCPVNIEILDKILDMRRYLTLMESD
                     FPTELGTAFRAMENSGNPWGMSQAERGDWAKDLEGIDIIDGSDPMTAEYLYWVGCAGS
                     FDDKNKKVTQAMAKLLQRAGVSFAILGPAENCTGDPARRSGNEYIFQMLAMQNIETLD
                     GMGVRKIITQCPHCFNTIGNEYPQLGGHYEVIHHSQFLEQLIDSGRLDLSGAKLEERI
                     VYHDSCYLGRHNDVYLAPRNVIGRLGGVEIVEATRSGTKGMCCGAGGARMWMEEHIGK
                     QVNVERSQELVATGATRIATACPFCYVMIDDGVKAQGVEDDDVKVGDLAMHVLDAIEN
                     ADPLAQPPLAEAIEASED"
     gene            complement(9248..9667)
                     /locus_tag="PLZ72_07405"
     CDS             complement(9248..9667)
                     /locus_tag="PLZ72_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNA36155.1"
                     /translation="MSQLTEHLARIPLLAGCSSRDLELLGRAADQITLRGGSTLTRQG
                     DIGREAFVLLDGTVEVRRDDRPVAELGPGAVIGELALLDGGPRTATAVATTDVTVLVL
                     TRPAFNAVLDEIPTLAHRLLVTLARRLRDAEEPDQIL"
     gene            9919..10767
                     /locus_tag="PLZ72_07410"
     CDS             9919..10767
                     /locus_tag="PLZ72_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNA36156.1"
                     /translation="MRVVAVAGAKGGVGKTAAAVNLAMVASAAGYRTLLWDLDPQGAA
                     THCYRARARVKGGATRLLGGKRGLDSYIRSTDHVRLDLLPADPSFRVVDTVLSSRRWP
                     DRVIRKLLRPLDRSYDVVILDCAPGLGIVTESVVAASDLVLVPVVPAPLSVRSLEQLQ
                     AFIDEHRPGLPLLAFLSMLDDRKVLHRQVVGQLQGDPRVALSAVPVSSAVERMGLEQV
                     PAVLASPLNLAAVAYRRLWAEVEDRLDLDAPSGSVIDLTGPAAPSDGSARPEAPLLSV
                     DQPPVA"
     gene            complement(10749..11510)
                     /locus_tag="PLZ72_07415"
     CDS             complement(10749..11510)
                     /locus_tag="PLZ72_07415"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HNA36157.1"
                     /translation="MHQLVLLRHGESEWNKLNLFTGWYDCDLTDAGRAEAAAAGPMLT
                     DAGLEPDVVHTSLQLRAIRTANLALESMGRLWVPVRRSWRLNERHYGDLTGRNKKETV
                     DKYGEEQVLVWRRSYDTPPPAIAADNGFNPNDDARYADLPPELVPTAECLADVVDRML
                     PYWYDSIVPDLAAGHTVLVAAHGNSLRALVKHLLDISDADITELNIPTGVPLALELDA
                     SFRPADDIPLEQRYLMDADAVKAKADAVARQATGG"
     gene            11675..12706
                     /locus_tag="PLZ72_07420"
     CDS             11675..12706
                     /locus_tag="PLZ72_07420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA36158.1"
                     /translation="MSASSAGTARAPVSVSVVIPCFNSLDYLPETVASVLAQDHRDLE
                     VVLVDDGGTDDLAGWVRDRAEPRVRLIRQDNAGPSAARNTGIAASDGELVAFLDSDDT
                     WEPDYLSRMVGCFDDPAVGLAYSGWDVVDAEGRPNGRATVSEWSGDVWDRFVTRNPVA
                     CSGAVVRRTVFDDVGVFAVNRDRFPIDVEDWEMWVRIAASHQVAVVAEVLVHHRRHGA
                     NSSSDPESLHEAYGHFLDTVFDGQPPERQALRPLATARAEIVLGWHSLADRRDPGRAL
                     AYRRSAARHAPEVRRAPDWWRLGAAAFALKVAGERGFRIVRDANQGARRVLARLPGPR
                     VRREGTFTP"
     gene            12938..14776
                     /gene="dnaK"
                     /locus_tag="PLZ72_07425"
     CDS             12938..14776
                     /gene="dnaK"
                     /locus_tag="PLZ72_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNA36159.1"
                     /translation="MPKAVGIDLGTTNSVVSALEAGDAVVIPNAEGARTTPSVVAFGK
                     DGEVLVGEVAKRQAVTNADRTIRSVKRHMGTNWKIEIDGKNYASQEISARILMKLKRD
                     AESYLGDTVTQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAALAYGMDKE
                     AEDQTILVFDLGGGTFDVSVLEIGDGVFEVKSTHGDTSLGGDDWDQRVIDWLVDSFKG
                     DHGVDLGNDKMAVQRLKEAAEKAKIELSQVAQTQINLPFITATDAGPLHLDYALSRAK
                     FQEMTADLIERCKVPFEQAIKDAGISKDQVDHVIMVGGSTRMPAVQELVEGLTGREPH
                     KGVNPDEVVAMGAAIQAGVLKGDVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTIPT
                     KKTEVFTTAEDSQPSVEIHVLQGEREMADFNKTLGKFQLVDLPLAPRGVPQIEVTFDI
                     DANGIVNVSAKDRATNKEQSITITGQSSLSKDQIDQMVRDAESHAEEDRRRKDEADVR
                     NRSDTLVYQTEKVLREQGDKVDPAEKETVEAALAELKDALNGSDLDRMNTASESLMAA
                     SQKLSQMLYEQASRDEAAGAGASAGGDAGASDDEVIDAEIVDEEGK"
     gene            14773..15408
                     /locus_tag="PLZ72_07430"
     CDS             14773..15408
                     /locus_tag="PLZ72_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNA36160.1"
                     /translation="MSSTGDGDASGRSPAPDDAPLDEDVAVGEVVDDLPDDSSAADDL
                     AALVDADLDLTADSLEDLGQTAEAAVVEAVDVAQRLEVERNELRDLVQRVKADFDNYK
                     RRQEVQRSEQRATAAMELVRELLPVLDACELAVAQGHADVAPVEAQLIGTLAKRGLVK
                     VTDVDVEFDPNVHEAVLHEEGDGTPVVVEVMRSGYLWNDHVVRAAMVKVKG"
     gene            15412..16542
                     /gene="dnaJ"
                     /locus_tag="PLZ72_07435"
     CDS             15412..16542
                     /gene="dnaJ"
                     /locus_tag="PLZ72_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNA36161.1"
                     /translation="MSSPQREWFEKNYYAVLGVSETAAQKDITKAYRQLARQLHPDAN
                     PDDPVAEERFKEVSAAYEVVGDPDKREQYDEVRRMGPVGAGFGGPGGFGPGGARFEGA
                     DLSDLIGNLFSRGRQRGGGPQAASTGPRRGSDIEATLTMTFDDAARGATTTVNVVSDI
                     RCPDCFGSGAAVGTAPLSCPDCGGTGAVAEDQGFFSFSSPCPRCGGRGRIIEQPCTTC
                     SGLGSVRRPRQVKVRIPQGVRDGQRIRVKGRGGAGANGGPDGDLYVRVQVEPHPLYGR
                     DGDNLLLSVPVTYPEAALGADVTVPTLDGDAVTIRVPAGTRSGRTFRVRGKGVATPKT
                     TGDLLVTIEIDVPTEIGDDERVALEALAAARTGPSPRAHLEV"
     gene            16545..16919
                     /locus_tag="PLZ72_07440"
     CDS             16545..16919
                     /locus_tag="PLZ72_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNA36162.1"
                     /translation="MPEFHQAVYVISVAAELAGVHPQTLRIYERKGLVDPGRTRGGSR
                     RYSEADIALLRRIQELTNDGLNLAGVKRVLELEDRVAELEERNRQLSAEIAERVDEVH
                     RQYRRDLVPLHQAVVRYERPRR"
BASE COUNT         2642 a         6067 c         5990 g         2334 t
ORIGIN      
        1 cgagtccgca tcgtcacccc cgacggccga cgcctcagcc tcggcaccca cgccagcaag
       61 cgcgccgccg aggcggcata cgcccggtcg gtcaccgacc aggccgacgg caagctcgcc
      121 accccgtcgg cggtcaccac gccgacgctc ggcgactacg cgtcgagctg ggtcgagacc
      181 cggctcacga agcgaggtga gccgctccgg ccacgggtca aggacctcta cctcatgcag
      241 ctgcgtctgc acatcgtccc gaccttcggc acgaggcgac ttgcccggat caccaccgcc
      301 caggtccgcg gctggaacgc ccgcctccga ggacccgacg gacccggcgc cagcaccgcg
      361 gccaagtgct accgcctgct ccggtcgatc ctgacgacgg ccgtggagga cggcctgatc
      421 ccggccaacc cctgcaccat caagggtgcc ggcgtcgaac cggccgacga gcgcccgatc
      481 cccaccgtcg cccaggtcca cgacctcgtc gagcacctcc ccgagcggat gcgatgcgtc
      541 gccctcctcg ccgccttcgt cggcctccgc cgaggcgaga tcctcggaat gcggctcggc
      601 gacgtcgacc tcgaccgccg cgagatcgcc atcgtccgcc agcgccagct cgaccgacac
      661 ggcaaccacc tcgtctgccc acccaagtcc gatgccggcc gacgagtcct caccatcccg
      721 gcggcgctcg cagtcgacct acgcggccac ctcgacgcct tcgcacagcc gggtgacgac
      781 ggctacgtct tcaccggcca gaagggcggc ccactcgcgc cgcacgtctg gcaggacgca
      841 tgggagaagg cccgcaccga ggttggcatc cccgagatcc acctccacga cctccgacac
      901 ctcgccggca ccctcgcagc gagcaccggc gccagcacca aggaggtcat gtaccgcctc
      961 ggccacggca cccaccaagc cgccctccgc taccagcacg ccacgaggca ccgcgaccga
     1021 gccatcgcag acgccctcga cgagttgatc gccccacgct cgaactgagc gagcgcaggg
     1081 cgatcccgcc cccagaggcc gtgctcggca ggtggtgctc ggccgcctat ccctcggtgc
     1141 cgggggcacc tggaggtggg ccgggctggg tgatgccaca cgcctcggac tcgaacgctg
     1201 cgatctcgtc ggctgctgcg gatgcttcgg gatcggcgaa gacggcaccg agatcgccgt
     1261 cggcagactc gaaggcagcg atgaacgtct cgacaggcgc cgcgatgtcc tgcggcgcga
     1321 gctgtgcgta ctcggcgagc tgctcgctgg ttggggtgac gccgacgagc tcggtggcgg
     1381 cagcgcagaa ggcgtcaagg tcatcggttg ccgaagcggt ggctgtggcc tcggcggtcg
     1441 tggtcgttga gtcggtgtcg gccgcgtcgt cggaatcgtc gctgcacgcg gcgatcggac
     1501 cgatggacgc tgcggcgatc agggcggcaa aggcgatgcg gcggacggcg gtggtcggca
     1561 tggttcctcc aggtgcagga tggggcccga cgggttcggg tctcggcggc tctgtcgctg
     1621 cgaacgctgt tgcgggatca ccgatctggg cgatgtgtga cgccggggtt ccggcgcggt
     1681 cgagcgtcgc gccacagggc tcgagcggcg accagcgcgg ctgccgtgcc gagtaccgtc
     1741 cagatgagca gggcccggtc ccagtcctcg tggggccaga ccgggaacag cggcaggtca
     1801 ggctcgaagg gccgggcgag cgcggcgcca gcgaccagcg gaaccgcggc agcggcgagg
     1861 gtcacccctg gttcgctgcg gcccgacgcc cgggcggccg tggccagccc gaacccgatc
     1921 ccacaggcga cagcaccttg caggatgagg acgtcgaggc ggtcgggaag gggcgctcgg
     1981 agggcccaat gggccgtggc ccagaagagg acgagagcga gcgggcccgc cgagcggacc
     2041 gcggtccgcc accagagcgg acgggggctg gcatccacca cggtcgcgca gcgctcgtcg
     2101 acggcgacgg cggccatccc gaccatgaga cccagggcgg tgccgtgcac cggccagagc
     2161 gcctccggcc accgagccac gagagcgacg ccggctccaa tgatgaggcc ggacccggcg
     2221 aggagcgacc agtgggtcgc cgcgacgagg tagcggagcg tcccgccgag atgcccgggc
     2281 ccgtcgttca tccgccccag gcgggcccat ccccgggcac cgggctgacg agcgtctcgg
     2341 ctgtcccggt gaacatggcg agcaccacgg tcaccaccgc caacccggcc agaacgatga
     2401 gcgcagtccg accacgggcg gaccgcgcct cgacatcggt cctgaccgcg acgaccgctc
     2461 cgatggcgca caggacgatc aagtaggtca gccaccagcc gggtgagccg ctgagcacaa
     2521 ccgagcgctg cgggtcgccg tcgatgccga acgggccacc ccagtaggtc caggggagca
     2581 cgacacggac acggcggagc ggttcgaaca ggccctggaa cacgatcacg gccaggacga
     2641 ggccgaccgt ggtcacgatc ggtgccgcgg cccgcttggt cttccgggct acgacgacac
     2701 cgaggatcgg tccgcccagg cagctcacgg cgccgagggc gaaccacaca gcagccaccc
     2761 acccgtccga catcatctcc gcgtacccgc cgcgaaccgt cggcaccgac tgcgcccgca
     2821 gcacggtcaa aagcacgaaa ccaaggccgg cgaggaacgg aacgacacag gcggccaggt
     2881 ggccgacggt acgcgatgcc tgaccgaccg gtgtcgggcc ggcggcctcc gcgcagcggt
     2941 ccgacgccca cacccgatgc gcgctcacgc tgaggccgac catcccgagc agagcggccg
     3001 gggcgatcat cgacagctcg tcggtcggct cgtcaccgtc gaggtagggc aagagcgttg
     3061 cgtaggtgag taccaacccg acgaggaacg ccgggcgacc ggcgtagcgg cggacctcga
     3121 tgcgggccag atccatccac ggcggccggg ccggctcggc caccgtgacg atcgcgggag
     3181 attcggctgc ggtcatgccg ccccctgcac ggcccgaggg ccgacgagga gcaggtaggc
     3241 gtcctcgacc gttggaggtg ccgcctgtgc atccgccggc ggggtgcccc cgacggagcg
     3301 cacgccgccg ccagggtcgc gccaggtcgc gaccgcgtcg gcaaccggtc gctcgccgag
     3361 ccacacccgg tcgcgggcgc tggccacgaa cccggcgacg tcgccgtcat gggcgacgcg
     3421 tccgccgacc aggacgatca cccggtcaca gactgcggcg acgtcctcgg tctggtgagt
     3481 ggcgagcagc acggtgccgt ggcgggcccg ttccgacagg gtcgaccgga acgtggcccg
     3541 ctgctcgggg tcgaggccgg tggtcggctc atcgaggatc agcaggtccg ggtcaccgat
     3601 cagcgcctgg gcgaaggcga gccgccgccg ctgaccgccc gacagcttcc gcaccttgat
     3661 cgtcggtcgg tcctccagac cgacgacacc caggacccgt cgcacctcgt cgtggcgggc
     3721 agcggtgtcg tcccacttct tgagcacggc gatgtagtcg aggaacccga acgccgtcat
     3781 cccagccggg aaccggacct cctggggcag gaagccgagc ctgcggcgga tctcggtgcg
     3841 ttccgccagc ggtccgtcgg cccgacggcc gagcacggtg atctcgcccg cgtcggcccg
     3901 ggatgcggtg ctcagcaacc cgagcagcgt cgtcttgccc gctccgttgg ggcccagcag
     3961 cgccgtgatg cccggcgaca ggtcgaagct gacgtcgtcg agcaccgcga ccgggccgta
     4021 gcgacgggtc acgtgtgaga cgcggacggt ggtgggcacg ggtccctcct tcaggttccg
     4081 ccgatggcgg acggggccgg gtcgctcgtg aggcgacggt ggagaagcag cgcagcgccg
     4141 accgagacgg cgactgcgac gagctgggtc gacgcggtcg tgatcgccca cggcgtgtcg
     4201 cgcacctgcc aggcgatgat cacaccggtc catccgaggg cgacgaatgc ggcgggccgg
     4261 accgggtcgg accaggtcga ggccgcggtg acgcacaacg tgcaggccag ggctggcagc
     4321 agccagctca ctgccgcccc cggatggtgc agcaccacgg cccccacagc gacggcgacc
     4381 ggcaccgcag tgaagacgac gtacgcggtg cgccacagca gcatccgcgc cggggagaaa
     4441 ggcgccgccc gctcgagcag gtggatgccg tggggcacga gccggtaggt cgcggcgacg
     4501 cccagcgcag gcagcagtgg ggcaacgatc aggaaggcgg cgtgggccga atcgaccgga
     4561 ccgagcaccg ccgccaccgc agtgaccgcg agcacaagcg tcgtggcgat cgtccactgc
     4621 cggctctgcg cggcgacgac gcgtgtcacc actacgtccg acatggacgc cccgaggcgg
     4681 agcaacgcct gcccgccgcg ccccagctcc ggggcggaga tcgcagcctc gatccgggtc
     4741 cacatctggt cgaggtcgag gtcgaggtcg gttcgacgat ggtcgccggt cgcgctggcg
     4801 gccaggaagg cacggcaatc ctcgcaccgg acggcgtggg cctcgaccga tgcccgctgc
     4861 acggcgtcga gtcggcgcgt gccgtaggcg gtcgccaccg tgtcgtccac gtgccaggcg
     4921 ctcatcgaag atctcccaac tgcaacgacg tgccggcgag ctcggtgcgc aggacgctct
     4981 tggccttgcg catccggccc ttcaccgttc cctcggggac gccaagcatc cgtcccgtct
     5041 ccttcgccgt cagtccgtcg aaaaccatcg cttgcaccac ctccatcagc tcgggcgaca
     5101 gcgatgccag cacggccccc gcctcgccga ggtcgatcga ctccagcacc cgctcctcgg
     5161 tgctcgcctc ggcaccctgt gccgggaacg gcgccggtcg agcctgacgt cccgcccggc
     5221 gcaggtgcgt gaccagttgg cgcacgccga tcccccagat ccacgcaccg acatcgccgt
     5281 cggctcgcca acgccccgct ccccgccaca ccgccaggaa cgtgtcctgc accgcgtcgg
     5341 ccaccagcgc atcgtcccaa caacgggcgc ggagccgggc aaccaaccac ggcgcatgcc
     5401 ggtcgtagag gacccgcagc gcagctgcgt caccggatgc gatggccgcg acgaggacga
     5461 cgtccctccg ctcgtcgccg gaaccgccag ccacgctcac cgtcgcacca ccgatgacct
     5521 catcgtcaca ctgtcgctcg aacactgcga tccggatcac gacgcgtcct gctcggatcg
     5581 agacggccga tccgtagggt cacgttaggg tcacgccgcc gatttcggcc catcaacgac
     5641 gaaggcccag gtccccggaa accgagcctg acctgggcct tcacagtgga gcgggtgacg
     5701 ggaatcgaac ccgcgttctc agcttgggaa gctgatgttc taccattgaa ctacacccgc
     5761 gtagttcgcc gacctggcct gacggccgac cggcgagcgc ccactgtagc gtccgcgccc
     5821 ggggctcccg tcccgcccac gtcgacccgc cgggcgggag ggcagcaggg cgccgactac
     5881 cgttcgggcg tgatcctctg cgaccggacc atccgtgccg agatcgaatc cggccgcatc
     5941 gtcgtcgacc cgttcgaccc cgccatggtg cagccgagct cgatcgacgt tcgcctcgac
     6001 caccggttcc tggtgttccg gagccacacc cgacccgtga tcgacgtgaa gcaggacctg
     6061 tcggacctga ccgagctcgt cgaggccgac gacgaccggc ccttcatcct gcaccccggc
     6121 gagttcgtgc tcggctcgac cgccgagcgc gtgggcatcc ccgacgacct ggtcgcccgc
     6181 atcgagggca agagctccct cggacgcctc ggcctcctca tccacaccac tgcgggcttc
     6241 gtcgacgccg gcttcgaggg ccacatcacc ctggagctgt cgaacgtcgc gacgctgccg
     6301 atcaccttgt atccgggcat gaaggtcggc cagatctcgt tcatccgcat ggacggcccc
     6361 gccgagcacc cctacggaac cggggcgttg ggctccaagt actccggtca ggtcggcccg
     6421 acccccagcc agtactggaa gaacttcgac gcctgaggcc ctccgcgtgt gggatcgacg
     6481 cgtcagccgt cgttctcaca cgcgaacgac ggtcgagggt gggcaatcag ctcagccggt
     6541 cgatcgcctc gaccaggcgt tcggcctcga ccggtcggat cagcaccgag tcggctccgt
     6601 cggccagcgc cgagtcggcc gcgtcggcgt cgtcgaccac gaccaccacc gacacgccgc
     6661 gctgacgagc cgagagcgag ccccgcaggt cggccaccag cgttgtcgcc ggagtggcca
     6721 gcacgatggt gctgggtggg tcggacagcg ccgtgccggc ggcgagcgcg accgagtcgg
     6781 tgagcaggac ctcggcgccc gccgctccca gcaggtcgcc cagcatgact ccgccgggat
     6841 cgccgggccg gaccacgacg acgacgtggc ccagcgtcac gaccgctccg tcagtcctcg
     6901 gaggcctcga tggcctcggc gagcgggggc tgcgccagcg ggtctgcgtt ctcgatcgcg
     6961 tcgaggacgt gcatggccag gtcgccgacc ttgacgtcgt cgtcctcgac gccctgggcc
     7021 ttcacgccgt cgtcgatcat cacgtagcag aagggacatg cggtggcgat gcgggtcgcc
     7081 ccggtcgcca cgagctcctg ggagcgctcg acgttgacct gcttgccgat gtgctcctcc
     7141 atccacatcc gggcaccgcc ggcgccgcag cacattccct tggtgcccga gcgggtggcc
     7201 tcgacgatct cgacgccgcc gaggcgaccg atgacgttgc gcggggccag gtacacgtcg
     7261 ttgtggcggc cgaggtagca cgagtcgtgg tacacgatcc gctcctcgag cttggcgccc
     7321 gacaggtcca accggccgct gtcgatcagc tgctccaaga actggctgtg gtggatcacc
     7381 tcgtagtgcc caccgagctg cgggtactcg ttgccgatcg tgttgaagca gtgggggcac
     7441 tgcgtgatga tcttgcgcac tcccatgccg tcgagcgtct cgatgttctg catggcgagc
     7501 atctggaaga tgtactcgtt gcccgagcgc cgggccgggt cgccggtgca gttctcggcc
     7561 gggccgagga tggcgaagct gacgccggcc cgctgcagca gcttggccat cgcctgggtg
     7621 acctttttgt tcttgtcgtc gaaggacccg gcgcaaccga cccagtacag gtactcggcg
     7681 gtcatcgggt ccgagccgtc gatgatgtcg atcccctcga ggtccttggc ccagtcgccg
     7741 cgctcggcct ggctcatgcc ccaggggttg cccgagttct ccatggcccg gaacgcggtg
     7801 cccaactcgg tcgggaagtc ggactccatc agcgtcaggt agcgacgcat gtcgaggatc
     7861 ttgtcgagga tctcgatgtt gaccgggcag ttctcgtcgc acgccttgca cgacgtgcac
     7921 gaccagatct cctcggtggt gatccgctcg aacagcaggt cggccgacac ggtgatctcg
     7981 gagtcgaccc cgatcggggg cgacacgacc gggttgccgg tggcggccat gacctcgccg
     8041 gtcttgagca cgatctcgcg ggggtcgagc ggcttgccgg tcgcgtgcgc cgggcacacc
     8101 gcggtgcacc ggccgcacat cgtgcaggcg tcggtgtcga gcaactgctt ccaggtgaag
     8161 tcctcgaccc ggtaggcgcc gaagctctcg agctcggtct ccatcaggtt gggcatggcc
     8221 ttcatggcgc ccttggggcg atccttctcc gacaggtaca tgttcagcgg agaggtgaag
     8281 atgtggcgca gcatcgtcac cggcaggatg accaggaagg cgacgaaggt gaggatgtgg
     8341 gcgacccaca tgacctggtg ccaggtgtcg aggttcgaca tgttctcgaa caccagcgcc
     8401 agcgggtagc cgatgaagct ccacttctcg aagctcggca tgccggtgtc cgcgatgcgg
     8461 aagccctcgg ccaggaaccc ggtgacgccg atagcggcca gcacgccgag gatgaccgcg
     8521 tgctcgggct tggtcttgat gcggatgcgg tacggccgct ggatgtagcg gcgcacgatc
     8581 gcccacgtga cgccggtgag gaacaccacg ccgcccaggt cgccgatgaa cgagaacgcc
     8641 tcgtacacgc cgccgtggag gaacttgatg ccctcgggca gctggtggtt gatctccagg
     8701 atcgaggtga ccgccaacag caccaggaag ccgaagtaga tcatcgagtg catgatcccg
     8761 gcggcggggt cgcgcagcag ggtctgcatg tacagcccgg cgcggagctt ctccatgcgc
     8821 ttcttggcgt tcttcggggt gatcgtccga cggtccgggc cgccgcgctc ccagttgcgg
     8881 acgcggttgg cgaacatcac cgagccgatg atgaacatga tcgggatgac cacgtagaac
     8941 gcgaacttca gggcgccggg gacacccccg aacacctcgc gctggatcgg cgagtcgtcg
     9001 tgccactgca gggtcgatgc cgcgatcccc gacaccacca cgatcagcgc gatgacgatg
     9061 ccgatggcga ccgagatctg gtgcggctgc agacgcttcg gtcccggaac cggcgtcgga
     9121 gcgtcggagt cgtgggtggc gtcggaggac gtgtcggtgg cactcatagg cactcgcgaa
     9181 cctagtccga cgccgtcatc cgcggtgaag tcctgacggt ccgtcagccc ggtccgcgtc
     9241 gtgggtctca gaggatctgg tcgggttcct cggcgtcgcg cagccgacgg gccagcgtca
     9301 ccagcaggcg gtgcgccagc gtggggatct cgtcgagcac cgcgttgaac gcgggcctgg
     9361 tcaggactag gacggtgacg tcggtcgtgg cgaccgcggt ggcggtgcgg ggcccgccgt
     9421 cgagcagggc gagctcgccg atgacggcgc ccggtccgag ctcggccacc ggccggtcgt
     9481 cgcggcggac ctcgacggtc ccgtcgagca gcacgaacgc ctcgcgcccg atgtcgccct
     9541 gtcgggtcag ggtgctgccg ccgcggagcg tgatctgatc cgccgcacgc cccaggagct
     9601 cgaggtcccg ggacgagcat ccggccagga gcgggatccg cgcgaggtgt tcggtgagct
     9661 gcgacacgtc cgggagacta cggggccggg gatccggcgc gtcggggatc gacgcggagc
     9721 gtcagggcgt cggagcgctc cggcatcgac accgggtgcc ctgtcggtcg gcttgaatgg
     9781 ggacgctggg agcaccgagg tgctcagggc cgggttcgac ggcagctcgc cgtggggtgc
     9841 ggccgatgcc gcgggggtcc gcggccgcgg ggcccgcgac ggggttcctg cgagccgatc
     9901 accaacagga gtgcgcagat gagggtcgtc gcggtcgcgg gagccaaggg cggggtcggc
     9961 aagaccgccg ccgcggtcaa cctcgcgatg gtcgcgtcgg cggccgggta ccggacgctg
    10021 ctgtgggacc tcgaccccca gggcgcggcc actcactgct atcgcgcccg ggcccgggtc
    10081 aagggcggcg ccacccgcct gctcggaggg aagcggggtc tcgacagcta catccgcagc
    10141 accgaccacg tgcgactcga cctgttgccc gcggatccgt cgttccgggt ggtggacacg
    10201 gtcctgtcgt cgcgtcggtg gcccgaccgt gtcatccgca agctgctgcg tccgctcgac
    10261 cgctcctacg acgtcgtcat cctcgactgc gcgcccggcc tgggcatcgt gaccgagtcg
    10321 gtggtggccg cgagcgacct ggtgctggtg ccggtcgtgc cggccccgtt gtcggtgcgg
    10381 agcctggagc agttgcaggc cttcatcgac gagcaccgcc cgggactgcc gctgctggcg
    10441 ttcctgtcga tgctggacga ccgcaaggtg ctgcaccgcc aggtggtggg tcagctccag
    10501 ggcgaccctc gcgtcgcgct cagcgcggtg ccggtgtcct ccgcggtgga gcggatgggc
    10561 ctcgagcagg ttccggcggt gctggcgtcg ccgctcaacc tggccgccgt ggcctaccgg
    10621 cgtctgtggg ccgaggtgga ggaccgcctc gacctggacg cgccctcggg ctcggtgatc
    10681 gacctcaccg ggccggcggc gccgtccgac gggtccgccc gtcccgaggc gccgctgttg
    10741 tccgtcgatc agcccccggt ggcctgacga gccacggcgt cggccttggc cttcaccgcg
    10801 tcggcatcca tgaggtagcg ctgctccagc gggatgtcgt cggcgggccg gaacgacgcg
    10861 tcgagctcga gcgccagcgg cacgccggtg gggatgttca gctcggtgat gtcggcgtcg
    10921 gagatgtcga ggaggtgctt gaccagcgcc cgcagcgagt tcccgtgggc cgcgaccagc
    10981 accgtgtgcc cggcggccag gtccgggacg atcgagtcgt accagtacgg gagcatccgg
    11041 tcgacgacgt cggccaggca ctcggcggtc ggcaccagct cgggcgggag gtcggcgtag
    11101 cgggcgtcgt cgttcgggtt gaacccgttg tcggccgcga tggccggtgg cggcgtgtcg
    11161 tagctgcgcc gccacacgag cacctgctcc tcgccgtact tgtcgaccgt ctccttcttg
    11221 ttgcggccgg tcaggtcgcc gtagtggcgt tcgttgagcc gccagctccg ccgaaccggg
    11281 acccacagcc ggcccatcga ctcgagcgcc aggttggcgg tgcggatggc gcgcagctgc
    11341 aacgaggtgt gcaccacgtc gggctcgaga ccggcgtcgg tgagcatcgg cccggcggcc
    11401 gccgcctcgg cgcggccggc gtcggtgagg tcgcagtcgt accagccggt gaacaggttg
    11461 agcttgttcc actcgctctc gccgtggcgg agcaggacga gctggtgcat gacggtctcc
    11521 ctggtcgagg tccgtcgccg gatcggagcg acgcccgatc gtggcgcatc ggtcgacgcc
    11581 ggtgccaggc gaagccgtgg ccggcgagcg cgcgaccgac cccgtggcca tcggtccccg
    11641 cggccgagca ccaggggctc ggtacactcc ccgcgtgagc gcgtcctcgg ccgggacggc
    11701 ccgggcaccg gtgtcggtgt cggtcgtgat cccgtgcttc aactccctcg actacctgcc
    11761 cgagacggtc gcgtcggtgc tcgcgcagga ccaccgcgac ctcgaggtcg tgctcgtcga
    11821 cgacggcggg accgacgacc tggccggctg ggtccgggac cgggccgagc cccgggtgcg
    11881 actcatccgc caggacaacg cgggaccgtc cgcggcgcgc aacaccggga tcgccgcgtc
    11941 cgacggtgag ctggtggcgt tcctcgactc cgacgacacg tgggagccgg actacctctc
    12001 ccgcatggtc ggctgcttcg acgaccccgc cgtgggcttg gcctacagcg gctgggacgt
    12061 cgtcgacgcc gaggggaggc ccaacgggag ggccacggtg tcggagtggt ccggcgacgt
    12121 ctgggaccga ttcgtgaccc gcaacccggt ggcgtgctcc ggggcggtcg tgcgtcggac
    12181 ggtgttcgac gacgtcgggg tgttcgcggt gaaccgcgac cggttcccga tcgacgtcga
    12241 ggactgggag atgtgggtgc gcatcgccgc gtcgcaccag gtggcggtgg tggccgaggt
    12301 cctggtccac catcgtcgtc acggggccaa ctcgtcgtcg gatcccgagt cgcttcacga
    12361 ggcctacggc cacttcctcg acaccgtgtt cgatggccag ccgccggagc gccaggcgtt
    12421 gcgaccgctc gcgaccgccc gcgccgagat cgtgttgggc tggcacagcc tggccgaccg
    12481 ccgggacccg ggacgtgccc tggcctaccg gcgctcggcc gcccgccacg cgcccgaggt
    12541 gcgtcgggcg ccggactggt ggcgactggg agccgcggcg ttcgctctga aggtggcggg
    12601 cgaacgcggg ttccggatcg tccgcgacgc caaccagggt gctcggcgcg tgctggcgcg
    12661 gttgcccggc ccccgcgtgc gccgcgaggg cacgttcacc ccctgagccg tcgtcagcct
    12721 gagcccgtct tcagcgaagt gggccgccgg agagcagacc tcacaaccag acactcaagt
    12781 ttggtatgct caacgctgtc acgaatgggc cggatcccgg aacggaccga taacctgagt
    12841 cgtcgacgag cagatctgtc gatgcgctca gcccaggatc cgccctcagc cccccccaac
    12901 atcccagacc gcaggaaacg gaaagcgaga cccccagatg cccaaggcag tcggaatcga
    12961 cctcggtacg accaactccg tcgtctcggc cctcgaggcc ggtgacgccg tggtgatccc
    13021 caacgcagaa ggcgcccgga cgacgccgtc ggtggtggcg ttcggcaagg acggcgaggt
    13081 gctcgtcggc gaggtggcca agcgccaggc ggtcaccaac gcggaccgca ccatccgctc
    13141 ggtcaagcgc cacatgggca ccaactggaa gatcgagatc gacggcaaga actacgcgtc
    13201 gcaggagatc tcggcgcgca tcctgatgaa gctcaagcgg gacgccgagt cctacctggg
    13261 cgacaccgtc acccaggcgg tcatcaccgt gccggcctac ttcgacgacg cccagcggac
    13321 cgccaccaag gaggccggcc agatcgcggg cctcgaggtg ctgcgcatca tcaacgagcc
    13381 gaccgccgcc gcgctggcct acggcatgga caaggaggcc gaggaccaga cgatcctggt
    13441 gttcgacctc ggtggtggga ccttcgacgt gtcggtgctc gagatcggcg acggcgtctt
    13501 cgaggtcaag tccacccacg gcgacaccag cctgggcggc gacgactggg accagcgcgt
    13561 gatcgactgg ctggtcgact cgttcaaggg cgatcacggc gtcgacctgg gcaacgacaa
    13621 gatggcggtc cagcggctca aggaagcagc cgagaaggcc aagatcgagc tgtcgcaggt
    13681 ggcgcagacc cagatcaacc tgccgttcat caccgccacc gacgccggcc cgctgcacct
    13741 cgactacgcc ctgtcgcggg ccaagttcca ggagatgacc gcggacctga tcgagcggtg
    13801 caaggtcccc ttcgagcagg cgatcaagga cgccgggatc tccaaggacc aggtcgacca
    13861 cgtgatcatg gtcggcggct ccacccgcat gcctgcggtg caggagctcg tcgagggcct
    13921 gaccggtcgt gagccgcaca agggcgtcaa ccccgacgag gtcgtcgcca tgggcgccgc
    13981 gatccaggcc ggcgtgctca agggcgacgt caaggacgtc ctgctgctcg acgtcacccc
    14041 gctctccctc ggcatcgaga ccaagggcgg cgtgatgacc aagctgatcg agcgcaacac
    14101 gaccatcccc accaagaaga ccgaggtctt caccaccgcc gaggacagcc agccgtcggt
    14161 cgagatccac gtgctgcagg gcgaacgcga gatggccgac ttcaacaaga cgctcggcaa
    14221 gttccagctg gtcgacctgc ccctcgcccc ccggggcgtg ccgcagatcg aggtcacctt
    14281 cgacatcgac gccaacggca tcgtgaacgt gtcggccaag gaccgcgcca ccaacaagga
    14341 gcaatccatc acgatcaccg ggcagtcgtc gctgtccaag gaccagatcg accagatggt
    14401 ccgcgacgcc gagagccacg ccgaggagga ccgccggcgc aaggacgagg ccgacgtccg
    14461 caaccgctcg gacaccctcg tgtaccagac cgagaaggtg ctccgcgagc agggcgacaa
    14521 ggtcgacccg gccgagaagg agacggtcga ggcggctctg gccgagctca aggacgccct
    14581 caacggctcg gacctcgacc gcatgaacac cgcgtccgag tcgctgatgg ccgccagcca
    14641 gaagctgtcg cagatgctct acgagcaggc ctcccgtgac gaggcggccg gtgccggtgc
    14701 atccgcgggc ggcgacgccg gtgcgtccga cgacgaggtc atcgacgccg agatcgtcga
    14761 cgaggagggc aagtgagctc cacgggcgac ggggacgcgt cggggcgttc ccctgcccct
    14821 gatgacgccc cgctcgacga ggacgtcgcg gtcggggagg tcgtcgatga cctccccgac
    14881 gacagctccg ctgccgatga cctggccgcg ctcgtcgacg ccgacctcga cctgaccgcg
    14941 gactcgctcg aggacctggg gcagaccgcc gaggccgccg tcgtggaggc cgtcgacgtg
    15001 gcccagcgac tcgaggtcga gcgcaacgag ctgcgcgacc tggtccagcg ggtcaaggcg
    15061 gacttcgaca actacaagcg tcgccaggag gtgcagcgct ccgagcagcg cgccaccgcc
    15121 gcgatggagc tggtccggga gctgctcccg gtcctcgacg cctgcgagct cgccgtcgcc
    15181 cagggccacg ccgacgtggc cccggtcgag gcccagctga tcgggacgct ggccaagcgc
    15241 ggcctggtca aggtcaccga cgtcgacgtc gagttcgacc cgaacgtcca cgaggcggtc
    15301 ctgcacgaag agggcgacgg caccccggtc gtcgtcgagg tgatgcgctc gggatacctg
    15361 tggaacgacc acgtcgtgcg ggcggcgatg gtcaaggtga agggctgacc ggtgtcctcc
    15421 ccccagcgtg agtggttcga gaagaactac tacgcggtgc tcggcgtgtc ggagacggcc
    15481 gcccagaagg acatcaccaa ggcctaccgt cagctcgccc gtcagctgca ccccgacgcc
    15541 aaccccgacg accccgtcgc cgaggagcgc ttcaaggagg tctctgccgc ctacgaggtg
    15601 gtcggcgacc ccgacaagcg cgagcagtac gacgaggtcc gccgcatggg ccccgtcggc
    15661 gccggcttcg gcggtccggg tgggttcgga cccggtgggg cccgcttcga gggcgcggac
    15721 ctgtcggacc tgatcggcaa cctgttcagc cgcggccgcc agcgtggcgg cggcccgcag
    15781 gcggcgagca ccggaccccg gcgcgggtcc gacatcgagg ccaccctgac catgaccttc
    15841 gacgacgccg cccgcggcgc gacgaccacg gtcaacgtcg tgtccgacat ccgctgcccc
    15901 gactgcttcg gcagcggcgc cgcggtcggc accgctccgt tgagctgccc ggactgcgga
    15961 gggaccgggg cggtggccga ggaccagggc ttcttctcgt tcagctcgcc gtgtccccgc
    16021 tgcgggggtc ggggtcgcat catcgaacag ccgtgcacca cgtgctcggg cctgggctcg
    16081 gtgcggcgcc cacgtcaggt caaggtccgg atcccccagg gcgtgcggga cggacagcgg
    16141 atccgggtca agggccgggg tggcgcggga gccaacggcg gtcccgacgg agacctctac
    16201 gtccgcgtcc aggtcgagcc ccacccgctc tacggccgcg acggcgacaa cctgctgctt
    16261 tccgtgccgg tcacgtaccc cgaggccgcg ctcggagccg acgtcaccgt gcccaccctc
    16321 gacggcgacg cggtgacgat ccgggtcccc gccgggacgc gcagcggtcg gaccttccgc
    16381 gtccggggca agggcgtggc caccccgaag accaccgggg acctgctcgt caccatcgag
    16441 atcgacgtgc ccaccgagat cggcgacgat gaacgggtag cgctcgaggc gttggccgcc
    16501 gctcggaccg gtccgtcacc ccgagcccac ctggaggtgt gaccgtgccc gagttccacc
    16561 aggccgtcta cgtcatctcg gtcgccgcgg agctggcggg cgtgcatccc cagaccctgc
    16621 gcatctacga gcgcaagggc ctcgtcgacc cgggtcggac ccggggtggc agccgccgct
    16681 acagcgaggc cgacatcgcc ctgctgcggc ggatccagga gctgaccaac gacggcctca
    16741 acctggccgg ggtcaagcgc gtgctcgagc tcgaggaccg cgtcgcggag ctcgaggagc
    16801 gcaaccggca gctgtcggct gagatcgcgg agcgggtcga cgaggtccac cgccagtacc
    16861 gccgggatct ggttccgctg caccaggccg tggtccgcta cgagcgcccg cgccgctgag
    16921 ccggtttccg ctcggccgtc gccccgatag cgttgagcgc cgtgtcgcgc cctctccgtc
    16981 ccgcgcccgg cctggcccga gcgcgaacaa ccccaccggc gccaccgccc cca
//
LOCUS       DAOQOD010000057        16383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1258_length_16383_cov_12.146497, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000057 DAOQOD010000000
VERSION     DAOQOD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_16383_cov_12.146497"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLZ72_07445"
     CDS             complement(<1..509)
                     /locus_tag="PLZ72_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNA36163.1"
                     /translation="MRRGGVALVLVGLLVAGVVASCGARVDVGVDGVSTRWIRVAAPQ
                     PRRALLVTDADRSPSDSDGLPLVLVLHGLGQNAEIMAPLGAFSSLARDGRAVVVFPDG
                     WHRSWNAGRCCGDAVGRGVDDVAYLNALIDEVVSETGADPRQVHMVGFSNGGMMVYRY
                     LCEGATRLRG"
     gene            657..788
                     /locus_tag="PLZ72_07450"
     CDS             657..788
                     /locus_tag="PLZ72_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36164.1"
                     /translation="MADGRRERHGPARWLLGRLIDRDHHDHCTYDDCPSDELDHASR"
     gene            814..2259
                     /locus_tag="PLZ72_07455"
     CDS             814..2259
                     /locus_tag="PLZ72_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36165.1"
                     /translation="MRQVGGPGEETVTGVAGRDDVVLGIGTTTGVVPDPPRPGTRSAT
                     FVDVVAAADGVPKSTTRSDQARTTDGRAIASGTVGTTQPTPDSQGGGGGQVSRVSMAC
                     GSTPSSERPDPAVVAPAGGSDGWCAPVGEDGTLGPAQLIGSDADDAVAGVTVHSDLGD
                     SAGAGTPDPSAFAVGRTSGLFPGAKDPTGGYLGEGDALVLRLDPTGSVRWARQFGTVA
                     TDEADAVTTSDDGDVIVGGSTDGRTDGDLVGTLGARDAWMARIDPSGNLRWLTQFGSP
                     GADRVRAVGRGGDPLRGTELFVGGGSTGGSIGSTTSLGGLDAMTAAFDASGRLVWSGQ
                     FGSPRDDEVTGVVVDGSTVYVSGTTDGEIVGAERISLTPTDEPDPGEGPTTAPSTTAP
                     PTTAPPTGGGRDSFLAAIDAASGEVRWVAQFGTAGEEQTTAMTRTESGLLVVSGSTTG
                     QLGATPPGGGTDGFMIAFRLPATGGGAASIV"
     gene            complement(2309..3859)
                     /locus_tag="PLZ72_07460"
     CDS             complement(2309..3859)
                     /locus_tag="PLZ72_07460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNA36166.1"
                     /translation="MAITDPRNDVTEPSAAVVVDGGAVEIAPPDLQQMSVPGRHERGP
                     VLSHLSLKMVLLKAGVVLADVLTIVVAYTVTTIVVGSGAAWSTGQIDDHLRIAALTLP
                     VWPVLFARQQMYASRFLTRLMDELRRVVHVVFVGTVALVLIGWVAGMDLDHIWVLCFL
                     LVALACVTLERFLIRRWFVHRRRTGRSLRDVVIIGTNAEALDLAEALQNPALGYRLVG
                     FVATDPAEPTDPADVGGLPVLAGTADTVSFAHALEAQGVILATTSLELGLSNRLLREL
                     LDGGLHVEITSGLRDVTPERMTVRPLGRHPVVYLEPARRFGWRAVAKRIFDTVLSSVA
                     LIVAAPVLIVAAIAIKITSPGPVLFRQVRVGRDGEPFFVLKLRTMVVDAEDRLAELMA
                     QNEADGPLFKMSDDPRITRVGRILRKLSIDELPQLWNVVRGDMSLIGPRPALPREVAQ
                     WSDELHERLRVRPGITGMWQVSGRSDTGFEEYQRLDLFYVDNWSILIDMGILLRTVPA
                     VLTGRGAS"
     gene            complement(4427..5713)
                     /locus_tag="PLZ72_07465"
     CDS             complement(4427..5713)
                     /locus_tag="PLZ72_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36167.1"
                     /translation="MRGPAGDTPKGRAARARIVEAAWDLLVHDGSVPLTAGLTVRSVA
                     AAAGMSPGAVQHHFPTMADLSDAMVDALLTDISVGPTDAVGDGMAQFDSDGLAAAVRA
                     AAQFNWDIINSVEELDIERRLRRVLILALGPGASAAALRSRLRSELWGECRGLIEHML
                     QALLDSAGRRLVDPITVADMAQLSGALADGLLTERLSGDVEIRSGLYADAVVALMQAL
                     SVPDGVQRTFADVDAGLHRLATRPAAPDTTDPVDVACAAAGLFSEGYADVGLAAVAAA
                     AGADVSVESIVDAFGTSRSVAAVSFARHLDAVVAAAVRRRDTSVEVALSDVVTELARR
                     AQAEPWVAAALVHERVLARASDPDRLVGLDVRRAVPLDDLIGDLLAELRPGWERREVD
                     ATAALVVDTVLNQSAGRSEEALGALVARVLRLVPSD"
     gene            complement(5754..7106)
                     /locus_tag="PLZ72_07470"
     CDS             complement(5754..7106)
                     /locus_tag="PLZ72_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36168.1"
                     /translation="MDHRGAAAAERWGLDPYGVGAAAAAPDPAEVDLDSIDLCDLARF
                     EHGFPHDTFAALRERRPVWYHPPGPHVPGDDGFWVLSRHADVLAAASDPVTFSSVGGP
                     GREGGGTLIEDLPVGYTGVLLNMTDDPHHQRFRSLLTPALSPRAIRSLEADIRRRSDG
                     IVAAALSRSEEAGEVDLLVHVAAELPLQAAASLIGVPQEDRARLIEWADATLDHDDHD
                     LGGTSDRAIAASAAMFEYGTTLIEARRDDPRDDLLSRAVHGEIDDESAPGGRRRLRPD
                     ELQLLFSLLVAAGTETTRNTIAVGVAALAARPDDWVRLREDRSLLPGAVEEMLRWASS
                     TPCNRRTATVDVDVHGQLIRAGDRVTLWWASADRDRSEFADPFRFDVTRAPNRHLAFG
                     HGSHFCLGSRLARLEIAAIVDSLLDRVEPPEQLGPIEWTRSNKHTGVRHLPVRMVARR
                     "
     gene            complement(7269..7817)
                     /locus_tag="PLZ72_07475"
     CDS             complement(7269..7817)
                     /locus_tag="PLZ72_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNA36169.1"
                     /translation="MIIVVVVLVVVIGLLVVVALNKLRQQRVSVDEAFAGIDVQLTRR
                     AELIPNLVETVKGYMGHERGTLEAVTQARAAAQSANTVGEKAAADGQMRQALANLFAV
                     AEAYPDLKASTNFQQLQGELTRTEDQLAFARQYYNDACATLNRTMVTIPWSLFAGMSG
                     VEKGVFYDAPEGHSAPPTVSFG"
     gene            complement(7915..9636)
                     /locus_tag="PLZ72_07480"
     CDS             complement(7915..9636)
                     /locus_tag="PLZ72_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNA36170.1"
                     /translation="MKSRRRTAAAWVIRVLVLAAVAVVWFVPPLSEKTSDTATISRYD
                     AVFDLSADGSLKITETIDVQMPYGKHGIFRIFDTRDERRNGVEHPVRDLTVTRDGQPE
                     PYQWDDAPAGQESLRIGSAAVTLTPGQHTYVITSTTTDTLERGPGGTVRWWWNVVGTG
                     WQMSMESVTATVHLPAEVTRVECVQGKDTPCRADASERTLTVRTGPLDPYTPVTVRAT
                     FPEGALPPPAPDPADHTVLWSIVAAVAAVALGAWFIVATRERPPGFPVLFEPPSGVSP
                     ALGAMVLDEVPSDDDLQATFYDLGAKGVIRLDGNDSAWNINLLVDPQDPRVSEMERGL
                     LVGLGLTAAGDAFLVSKSADAGEKLTTARATMRAIVARQSGEYLKPSGVGVLGTLLGW
                     VATVGVFVLAGLRLFGQGTWLGWPLFFGLAAFSLVAVTVSTDPGSRSKRTPEGRELWS
                     RAGGFARFLTTDSAESRFEAAKHMDWYPTYLAWALVFGSADAWAQRYLDQGVEPPALP
                     WIYWTGTHHYGAATFASSMANSFDAAISSANASYAASQASSGSGSFGGGGFSGGSGGG
                     GGGGGSW"
     gene            9717..10679
                     /locus_tag="PLZ72_07485"
     CDS             9717..10679
                     /locus_tag="PLZ72_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36171.1"
                     /translation="MNDALDPRPRPRSPRVALATSTEAWSLDEDAPLLTAALADVGLD
                     ARGVVWDDPDVDWSAFELVVIRSTWDYATRHREFIEWTERVQAVTDLRNPAAVIAWNT
                     DKRYLADLADDGVAVTPTTFLTTPMPEAEVSERIAESVGSDRGIVVKPTVSAGSKDTA
                     RYASRAEDPHALGAAATHAASLLDRGRAVMVQPYLDSVDDDGADGGETGLVFLGGTFS
                     HAFRKGPLLALGADPVAGLYAEETISAREPSDEQRAVAASVLDAARARTGFTELYARV
                     DLLPGEDGSPVLLELELCEPSFFLTSSPGSAARAAEAIAAAVRR"
     gene            complement(10693..11358)
                     /locus_tag="PLZ72_07490"
     CDS             complement(10693..11358)
                     /locus_tag="PLZ72_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNA36172.1"
                     /translation="MQKPLVRLVAAILAVLLVVGVGVAAIAPMAGAHGDDGVLAVVSA
                     TPSGSSSTITVKLTFEGDGHPVDGATVTAVADDGAGAAIDPVPMTAAGSPGEYTARLE
                     FPSAAVWNVRVTAVSPSATVTLTQDIAGPASTSSTPTGSTPTSGSTPGSTPASTSGAP
                     VDGVTATREDTAEEAVEQSDSSPLPWVLGIGALVVVVGAVAWVVARRGSGPDGPDRPD
                     DSA"
     gene            complement(11433..13085)
                     /locus_tag="PLZ72_07495"
     CDS             complement(11433..13085)
                     /locus_tag="PLZ72_07495"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNA36173.1"
                     /translation="MIRTRRRPLLWLVVGLGLLAAAGPLAGTASAHASLVSSTPGNDE
                     VVEQAPREVVLTFDDGVNVDDGGVRVLDPAGEDVAEGSPSTTADGTEVRQPVASGPEG
                     TYTVSYRALSDDGHVISGSYVFHVGRRTGSAAAAAAGGFDALRVLNAFGRWIALTGGL
                     LVGGVLAMALFVDGPRVRRQHPTDGDADTGAGSGPQGWGSELASARFLLLPGAVSVLF
                     GVGLSLLASAADLAGGSILDGPGATGRFLGASWAGTVAGLRVLVALVLVLAVLPPRAL
                     RRAPWLAAIGILAALALPSLGGHAWTASPVGLAVASDALHVLAAAAWVGALGVLVLSW
                     DGTRERAAAFSRMALVAAPLTVATGVLNAALQERSLSALTDTTHGRLVLAKALGTVAL
                     LAFGWVHRRQLADAARWSARVLRSYRAEVLVGLAVVAVTAVLVGTPPGREAARSAEPV
                     QMVKQAGDTTVRMEVSPAAAGPNDVHVYYLARDGSLARVDAAELKVSTGGVAPRAVPI
                     TPVTASHGVAEGVQLTPGDWTFELTVVVGGAPASTTFRVPIA"
     gene            13225..14070
                     /locus_tag="PLZ72_07500"
     CDS             13225..14070
                     /locus_tag="PLZ72_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA36174.1"
                     /translation="MTDEPTQTPPAVDRVTYAVDGGVATITIDAADAGNSLTSSMRDG
                     MTELVEWASADHGVRAIVLTGAGERHFCTGANLGGPQKPTPPRPEGAPDRLLGDGARM
                     IRRGWQRLIASILDSEKPVVARVNGTAAGGGAHLVLACDLVVMADTAKLVEVFVRRGI
                     LPDAGGAWMLPRIVGPQVTKELMFLADDVSAERCRELNIANRVAPAAELDAAVGELTG
                     RLVQAPTKALGLTKWLVNRAFESSRHTAFEEEAAAQEWLMHSEDFQEGLAAFRERRSP
                     EFRGW"
     gene            14241..14939
                     /locus_tag="PLZ72_07505"
     CDS             14241..14939
                     /locus_tag="PLZ72_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36175.1"
                     /translation="MALVLLAGLTTACGAGAAEPLRPLGNASAPRATTPGAATDPTGT
                     PPDSITPNAPEPAGPTTTAPEPGDGPGHVVADVLDRYDRVLARLHADPAAMNRPGDPV
                     GSELLGELGRVVPAGAVLFDDIRSQLAGRAARGESIPGEDRQLPYVHHAIDADVEGAD
                     RVTFTWCSWSPGVVRDTATGAVVDDTVGHGTGTGVAERIAGTWMLSSLDESTLDLLPA
                     GTADPCPGADRSRP"
     gene            15002..15859
                     /locus_tag="PLZ72_07510"
     CDS             15002..15859
                     /locus_tag="PLZ72_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36176.1"
                     /translation="MAFGGPSSAGAAGDSTASSRVTRDGRIITSIINRTPIRYPGRGT
                     APSTYWLTLTDTELSFLLAVLANDPRLQGDFVDVMSTLADAGPDRTADVQVQIRRGRF
                     TGQTRAVPTDRSAPAQVLARRMITSLPPLRPVQSPPTATPVVIGEPAFTSFDPGTWAT
                     VVDRSLTAGAVTARVRARPVSFRVRSGDPADNRMAICHGPGRPFDPTDPAPPGRQARR
                     AGTCAVTYLTRTGVEGRRDRWYGELTVIWRAEWTTDGITWRSLGDIPQIAVVGRTVVP
                     ATTAIESRS"
     gene            complement(15876..>16383)
                     /locus_tag="PLZ72_07515"
     CDS             complement(15876..>16383)
                     /locus_tag="PLZ72_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36177.1"
                     /translation="SAPTTPPSPDADAAPAESAGRDHGGSHRLLDEALAALVHNGVLT
                     QAQADAVTAEVTQRAEAARSERRDAWDARRQEMLDAAASVFGLTGEQLADELKAGKTL
                     DQLASEHGVDAQKISDALVTGADEQIDAAVAGGHLDAARAAELKEKFVQRIPDLVDAG
                     AALFGGRD"
BASE COUNT         2370 a         6007 c         5876 g         2130 t
ORIGIN      
        1 ccccggagtc gggtcgcccc ttcgcagagg tagcggtaga ccatcatccc gccgttcgag
       61 aacccgacca tgtggacctg tcggggatcg gcaccggtct ccgagacgac ctcgtcgatc
      121 agcgcgttca ggtaggcgac atcgtcgact ccccgtccga ccgcgtcccc gcagcaccga
      181 ccggcgttcc agctgcggtg ccagccgtcg gggaagacca ccaccgcccg gccgtcccgg
      241 gccagcgagc tgaacgcccc gagcggggcc atgatctcgg cgttctggcc cagtccgtgg
      301 agcacgagca cgagcgggag accgtcggag tctgatggcg acctgtccgc gtcggtgacc
      361 agcagcgccc ggcgcggctg cggtgccgcg acccggatcc agcgtgtcga caccccgtcc
      421 actccgacgt cgacacgggc cccgcacgac gcaacgaccc cggcgacgag aaggccgacg
      481 agcaccagtg cgacgccacc tcgacgcatg catgcgcacc tgctccatcg gccgcccgtc
      541 ctcacgggcg gggagcgtac cgactgcggt ccgagcgcga tgccgggctc ctgacaccga
      601 tgacgggtca cgagcggtga tactgctcgg atgcgtctgc gaccggtgac cgtccgatgg
      661 ctgatggtcg tcgcgagcgc cacggtcctg ctcgctggct gctcggacga ctcatcgacc
      721 gcgaccacca cgaccactgc acctacgacg actgcccctc cgacgagctc gaccacgcct
      781 cccggtgacg acgggccgct cccggtcgcc tgggtgcgcc aggtcggcgg cccgggcgag
      841 gagacggtga ccggcgtcgc cggccgcgac gacgtcgtgc tgggaatcgg cacgacgacc
      901 ggcgtggttc ccgatcctcc tcgaccggga acccgctcgg ccaccttcgt ggacgtcgtg
      961 gccgcggcgg acggagtccc gaagtcgacc acgcgctcgg accaggcccg cacgaccgac
     1021 ggccgagcga tcgccagcgg gaccgtcggc acgacgcaac cgacacccga cagccaggga
     1081 gggggcgggg gccaggtctc gagggtgagc atggcgtgcg gttcgacacc ctcctccgag
     1141 cgacccgacc cggccgtggt cgctccggcc ggaggaagcg acggttggtg cgccccggtc
     1201 ggagaggacg ggacgctcgg gccggcgcag ctcatcggca gcgacgccga cgacgccgtt
     1261 gccggcgtga ccgtccactc ggatctgggt gactccgccg gcgccggaac cccggatccg
     1321 tctgcgttcg cggtgggacg gaccagcggg ctcttccccg gcgccaagga tccgaccggc
     1381 gggtatctgg gcgagggcga cgccctcgtc ctccggctgg atccgaccgg ttcggtccgc
     1441 tgggcacgcc agttcggaac ggtcgcgacg gacgaggccg acgccgtcac gaccagcgac
     1501 gacggagacg tgatcgtcgg tggctccacc gacggccgca ccgatggcga cctcgtcggc
     1561 accctcgggg cccgcgatgc ctggatggct cggatcgacc cgagcgggaa ccttcggtgg
     1621 ctgacccagt tcggctcacc aggcgccgat cgggtgcggg cggtcggtcg tggaggcgat
     1681 ccactgcgag gaaccgagct cttcgtcggc gggggctcca ccggcggctc gatcggatcg
     1741 acgaccagcc tcggcggtct cgacgccatg accgctgcgt tcgacgcgtc cggacggttg
     1801 gtgtggtcgg gccagttcgg ctcaccacgc gacgacgagg tcaccggggt cgtcgtcgac
     1861 gggtccaccg tgtacgtcag cggcaccacc gacggcgaga tcgtcggggc cgaacggatc
     1921 tcgctgacgc cgaccgatga accggacccg ggcgagggcc cgacgacggc accctcgacc
     1981 acagcacccc cgaccacagc acccccgacc ggtggaggcc gggacagctt cctggccgcg
     2041 atcgacgccg cgtccggtga ggtccggtgg gtggctcagt tcggaaccgc cggcgaggag
     2101 cagaccaccg ccatgacccg caccgagtcg ggactgctcg tggtgtcggg ctcgaccacg
     2161 ggccagctcg gggcgacccc gcccggaggt gggaccgacg gcttcatgat cgccttccga
     2221 cttcccgcga ccgggggcgg cgcggcgtcg atcgtctgag caggagcaga cccgagttcg
     2281 atcccgcccc gcggaacgac gcctgaggtc agctcgcgcc gcggccggtg aggaccgccg
     2341 ggaccgtgcg cagcaggatc cccatgtcga tcaggatcga ccagttgtcg acgtagaaca
     2401 ggtcgagtcg ctggtactcc tcgaagccgg tgtcgctgcg cccgctcacc tgccacatgc
     2461 cggtgatccc cggtcggacc cgcaaccgct cgtgcaactc gtcgctccac tgcgccactt
     2521 cgcggggcag ggcggggcga ggaccgatca ggctcatgtc acccctgacg acgttccaca
     2581 gctggggcag ctcgtcgatc gacagcttgc gcaggatccg gccgacccgg gtgatccggg
     2641 ggtcgtcgct catcttgaac agcggaccgt cggcctcgtt ctgcgccatc agctcggcca
     2701 gacggtcctc ggcgtcgacg accatggtcc gcagcttcag gacgaagaac ggctcgccgt
     2761 cgcgaccgac gcgcacctgc cggaacagca ccggtccggg cgaggtgatc ttgatggcga
     2821 tcgcggcgac gatcaggacg ggggcggcga cgatgagcgc aaccgatgac agcaccgtgt
     2881 cgaagatgcg cttggccacg gctcgccatc cgaagcggcg ggcgggttcc aggtagacca
     2941 cagggtgacg accgagggga cgcacggtca tccgctccgg cgtgacgtcg cgtaggccgg
     3001 aggtgatctc gacgtgcagg cctccgtcga gcagttcacg caggagacgg ttggacaacc
     3061 ccagctcgag gctggtcgtg gccaggatca ccccctgggc ttcgagcgcg tgggcgaagg
     3121 acacggtgtc ggccgtgccg gccagcaccg gcagaccccc gacgtcggcc gggtcggtgg
     3181 gctcagccgg gtccgtggcg acgaagccga caagccggta cccgagggcc gggttctgga
     3241 gtgcctcggc caggtcgagc gcctcggcgt tggtcccgat gatcaccacg tcgcgcagcg
     3301 accgaccggt ccggcgccgg tgcacgaacc accgtcggat caggaaccgc tcgagggtca
     3361 cgcaggccag ggcgacgagc aggaagcaga ggacccatat gtgatcgaga tccatcccgg
     3421 cgacccaccc gatgagcacc agcgcgaccg tgccgacgaa caccacgtgc accacccgac
     3481 ggagctcatc catcaggcgg gtgaggaacc tggacgcgta catctgctga cgggcgaaca
     3541 ggacgggcca gaccggcagg gtcagcgccg cgatgcgaag gtggtcgtcg atctgccccg
     3601 tgctccacgc ggctccggag ccgacgacga tcgtggtcac ggtgtaggcg accacgatgg
     3661 tcagcacgtc cgcgaggacg acccccgcct tgagcaggac catcttgagc gacaggtgcg
     3721 agaggaccgg gccgcgctcg tggcgtccgg gcaccgacat ctgctgcaga tccggcggcg
     3781 cgatctcgac ggcaccgcca tcgacgacga cggccgcgct cggctcggtc acgtcgttcc
     3841 tcgggtcggt gatggccacg tcgagcgcct cgtagatcct gtcgttccgt gcacagggtc
     3901 gacggaacat gcgctccgtg tccccagtcg cttgctgtcg gtcatggacc ggctcggcgg
     3961 gccgatgtcg atccttacgt ccggctcagc gtaacgactc ccacggacac tgggatctga
     4021 gcagacgtgc ccgacgccac acatgggccg aatggcccac accgacctcc cgagccgtgc
     4081 tcccgagccg ctctgtagtg cgagatccag gtgttcacct ggatctcgca ctacagaacg
     4141 acgaaggcgc gggtccggct gccgggacga ccggtgccgg tccgactgat ggggcggggt
     4201 cggaccggca ccggtgggtc gacctgcacc ggggggtgcg gtcgaacgcc ccgatcgtgg
     4261 ccggaaccgc agcgggcgtc cacggatcga gggccgaatc ctcgaatccg ctcccgatcg
     4321 acccggaatt cccgcgcggc tgcatcggaa cggtccgtcc ggctccgccc tgaggcggga
     4381 cagagcccga gcggggccat cggcgccgct tctgacagaa gtgctgtcag tccgacggca
     4441 ccagccgcag cacccgggcg accagggccc ccagcgcctc ctcggaacgg ccggcggact
     4501 ggttcaggac ggtgtcgacc acgagcgccg cggtcgcgtc gacctcgcga cgctcccagc
     4561 ccggacgcag ctcggccagc aggtcaccga tcaggtcgtc gagggggacg gcgcggcgga
     4621 cgtcgaggcc gacgagccga tcgggatcgg aggctcgggc caggacgcgc tcgtgcacca
     4681 gcgccgcggc cacccacggc tccgcctggg cccgtcgggc gagctccgtc accacgtccg
     4741 acagggcgac ctcgaccgag gtgtctcgtc gtcggacggc agcagcgacc actgcgtcca
     4801 ggtgtcgggc gaacgacacc gcggcgaccg accggctggt gccgaaggcg tccacgatcg
     4861 actcgacgct gacgtctgcg cccgcggctg ccgccaccgc ggccaggccg acgtccgcgt
     4921 acccctccga gaacagcccg gcagccgcgc acgccacgtc gaccggatcc gttgtgtcag
     4981 gagcagctgg cctggttgcc agccggtgca acccggcgtc gacatcggcg aacgtccgct
     5041 gcacgccgtc ggggaccgac agcgcctgca tcagggcgac gacggcatcc gcgtacagac
     5101 ccgagcggat ctccacgtca ccggacaggc gctcggtgag gaggccgtcg gccaacgcac
     5161 cggagagctg agccatgtca gccaccgtga tcggatccac cagccgccgt ccagccgagt
     5221 cgagcagcgc ctggagcatg tgctcgatga ggccgcgaca ctctccccac agctcggacc
     5281 gcagccggct ccgaagtgcg gccgcgctcg ccccggggcc gagcgcgagg atgaggacac
     5341 ggcggagacg tcgctcgatg tcgagctcct cgacggagtt gatgatgtcc cagttgaact
     5401 gggccgcagc tcgcacggcg gccgccagac cgtcggaatc gaactgcgcc atgccgtctc
     5461 cgactgcatc ggtcggcccc accgagatgt cggtgagaag cgcgtcgacc atggcgtcgg
     5521 acaggtcggc catcgtcggg aagtggtgct ggaccgcgcc gggcgacatc ccggccgccg
     5581 cggcgacgct gcgaaccgtc aggcccgcgg tcaacggcac ggacccgtcg tggaccagca
     5641 gatcccaggc ggcctcgacg atccgggctc gcgcggcccg acccttgggc gtgtcgcccg
     5701 ctggtcccct caccgctccc ccctcctcct cgtgcggccg gtccccggcc gggtcaccgt
     5761 cgcgcgacca tccggaccgg caggtgtcgg accccggtgt gcttgttgga acgcgtccac
     5821 tcgatcggtc ccagctgctc gggcggctcg acccggtcga gcagcgagtc gacgatcgcg
     5881 gcgatctcaa ggcgggccag ccgcgacccc aggcagaagt gcgatccgtg gccgaaggcc
     5941 aggtggcggt tgggtgctcg cgtcacgtcg aagcggaacg ggtcggcgaa ctcggatcga
     6001 tcgcggtcag cggacgccca ccacaacgtc acccggtccc cggcccggat caattggccg
     6061 tgcacatcga cgtcgacggt agcggtccga cggttgcacg gagtcgagga cgcccagcgg
     6121 agcatctcct cgaccgcgcc gggcaggagc gaccggtcct cacgcagcct gacccagtcg
     6181 tcggggcggg cggcaagcgc ggcgaccccg accgcgatcg tgttgcgcgt cgtctcggtg
     6241 cccgccgcga ccagcagcga gaacagcagc tgcagctcgt cggggcgcag gcgccgacgt
     6301 cccccggggg cggactcgtc gtcgatctcg ccgtggaccg ctctcgacag gaggtcgtcg
     6361 cggggatcgt cacgacgagc ctcgatcaac gtcgtcccgt actcgaacat cgcggcgctg
     6421 gccgcgatgg cacgatccga ggtccctccg aggtcgtggt cgtcgtggtc gagggtggcg
     6481 tcggcccatt cgatgaggcg ggcccggtcc tcctggggga cgccgatcaa cgacgccgcc
     6541 gcctgcagcg gcagctcggc agccacgtgc accagcaggt cgacctcgcc cgcttcctcc
     6601 gagcggctca gcgcggcggc gacgatgccg tcggaccgcc tccggatgtc ggcctcgagc
     6661 gatcggatcg cacgaggtga cagcgcgggt gtgagaaggg accggaaccg ctggtggtga
     6721 gggtcgtcgg tcatgttgag cagcacaccc gtgtagccga cgggcaggtc ctcgatgagc
     6781 gtgcccccgc cctcgcgtcc ggggccgccg acgctggaga acgtgaccgg atccgaggcc
     6841 gccgccaaca cgtccgcgtg gcggctcagg acccagaacc cgtcgtcgcc ggggacgtgg
     6901 gggccgggtg ggtggtacca gacgggtcgg cgttcccgca gcgcggcgaa ggtgtcgtgg
     6961 gggaagccgt gctcgaagcg ggccaggtcg cacaggtcga tcgaatcgag gtcgacctct
     7021 gcagggtccg gtgcagcagc ggcggcgccc accccgtacg ggtcgagtcc ccatcgttcg
     7081 gcggcggccg ccccccggtg gtccacgtcg cgcacgctac cggctcggga tgaccgagcc
     7141 ggggaggacc cgtcgacggg atcggggtcg gtcgccgatg cggggttagg tggttcgtcc
     7201 cggtcgcccg gcgtctcgcc ttcggacagg ccgatccggt ccctgatccc ctacccgaaa
     7261 cgacagccct acccgaacga gaccgtcggg ggtgccgagt ggccctcggg ggcgtcgtag
     7321 aacacgccct tctccactcc ggacatcccg gcgaacagcg accacgggat ggtgaccatc
     7381 gtccggttca gggtggcgca ggcgtcgttg tagtactggc gggcgaaggc gagctggtcc
     7441 tcggtgcggg tcagctcacc ctggagctgc tggaagttgg tcgacgcctt gaggtcggga
     7501 tacgcctcgg cgaccgcgaa caggttggcg agcgcctggc gcatctggcc gtcggccgcc
     7561 gccttctccc ccaccgtgtt ggcggactgc gccgcagcgc gggcctgggt gaccgcctcg
     7621 agcgttcccc gttcgtggcc catgtagccc ttgaccgtct cgaccaggtt ggggatgagc
     7681 tcggcgcgac gggtgagctg cacgtcgatg ccggcgaagg cctcgtcgac cgacacccgc
     7741 tgctggcgaa gcttgttgag cgcgacgacg accagcaggc cgatgacgac caccagcacc
     7801 acgacgacga tgatcacgat ccacacggtg gttcctccct gatcgggcgc ccagtctgac
     7861 acggggtcga gcccaccggg cgaccggccg gagcggggac accgctgagc cgcgtcacca
     7921 cgagccgccg cccccgccac cgccgcccga gccgcccgag aaccctccgc cgccgaagct
     7981 ccccgacccc gacgacgcct gcgacgcggc gtagctggcg ttggccgagc tgatggccgc
     8041 gtcgaacgag ttggccatcg acgaggcgaa ggtcgcggcg ccgtagtggt gcgtgccggt
     8101 ccagtagatc cacggcaggg caggcggctc gacgccctgg tcgaggtagc gctgggccca
     8161 ggcatcggcc gatccgaaca ccagcgccca cgccaggtac gtcgggtacc agtccatgtg
     8221 cttggcggcc tcgaagcggg actcggccga gtcggtggtg aggaagcggg cgaagccccc
     8281 ggcgcgtgac cacagctcac gaccctcggg ggtccgcttg ctgcggctgc ccggatcggt
     8341 cgacaccgtc accgcaacca acgagaaggc ggccaggccg aagaagagcg gccagcccag
     8401 ccacgtcccc tggccgaaca ggcggaggcc ggccaggacg aacacgccga cggtggccac
     8461 ccacccgagc agggtgccca gcacgccgac cccggagggc ttcaggtact ccccggactg
     8521 gcgggcgacg atcgcccgca tggtggcgcg ggcggtggtc agcttctcgc cggcgtccgc
     8581 cgacttcgac accaggaacg cgtcgcccgc cgcggtcagg cccagcccga cgagcaggcc
     8641 gcgctccatc tcggacaccc ggggatcctg cgggtcgacg agcaggttga tgttccacgc
     8701 cgagtcgttg ccgtcgaggc ggatcacgcc cttggctccc aggtcgtaga aggtggcctg
     8761 gaggtcgtcg tcggagggga cctcgtcgag caccatggcc ccgagcgcgg gcgacacgcc
     8821 cgacggtggt tcgaacagca ccgggaagcc cggcggccgc tcccgggtgg cgacgatgaa
     8881 ccatgctccg agggcgaccg cggccacggc cgcgacgatc gaccagagca cggtgtggtc
     8941 ggcggggtcg ggcgcgggtg gcggcagcgc cccctcgggg aacgtggcgc ggaccgtcac
     9001 cggcgtgtac gggtcgagcg ggccggtgcg aaccgtcagc gtccgttccg acgcgtcggc
     9061 tcggcacggc gtgtccttgc cctgcacgca ctcgacccgg gtcacctcgg cgggcaggtg
     9121 cacggtcgcg gtgaccgact ccatcgacat ctgccagccg gtgccgacga cgttccacca
     9181 ccagcggacg gttccgccgg gaccgcgttc cagggtgtcg gtcgtggtcg aggtgatcac
     9241 gtaggtgtgc tgccccgggg tgagggtgac cgcggcgctg ccgattcgca gcgactcctg
     9301 gcccgctggg gcgtcgtccc actggtaggg ctcgggctgg ccgtcgcggg tgacggtcag
     9361 gtcccggacc gggtgctcca ccccgttgcg acgctcgtcg cgggtgtcga agatgcggaa
     9421 gatcccgtgc ttgccgtacg gcatctgcac gtcgatcgtc tcggtgatct tcagggagcc
     9481 gtccgcggac aggtcgaaca cggcgtcgta gcgcgagatg gtggcggtgt cgctggtctt
     9541 ctccgacagc ggcggcacga accagaccac cgcgaccgcc gccagcacca ggacccggat
     9601 cacccaggcg gcggcggtcc ggcgtcgcga cttcacggcc aggaccctat cgaccccacc
     9661 ccgttccgta gtgcgaggat cacacgttcg tgtggtcctc gcactacaaa gcgggggtga
     9721 acgatgccct cgacccccgt ccccgacccc ggagcccccg ggtcgccctg gccaccagca
     9781 ccgaggcgtg gtcgctcgac gaggacgccc cgctgctcac cgcggcgctg gccgacgtcg
     9841 gtctcgacgc ccgcggggtg gtctgggacg accccgatgt cgactggtcc gccttcgagt
     9901 tggtcgtcat ccgttccacc tgggactacg cgacccgcca ccgcgagttc atcgagtgga
     9961 ccgagcgggt ccaggccgtc accgacctgc gcaaccccgc cgcggtgatc gcctggaaca
    10021 ccgacaagcg atacctggcc gacctggccg acgacggcgt cgccgtcacc ccgaccacgt
    10081 tcctgaccac gccgatgccc gaggcggagg tgagcgagcg gatcgcggag tcggtcgggt
    10141 cggaccgggg catcgtggtc aagccgaccg tgtcggccgg gtcgaaggac accgcccgct
    10201 acgcctcccg ggccgaggat ccccacgcgc tcggcgccgc cgccacccat gccgcctcgt
    10261 tgctcgaccg gggccgggcc gtcatggtcc agccctacct ggactcggtc gacgacgacg
    10321 gcgccgacgg cggcgagacc ggcttggtgt tcctcggcgg caccttcagc cacgcgttcc
    10381 gcaaggggcc gctgctggcg ctcggcgccg atccggtggc gggcctgtac gccgaggaga
    10441 ccatctcggc ccgggagccg agcgacgagc agcgcgctgt cgccgcatcc gtcctcgacg
    10501 ccgcccgcgc ccgcaccggc ttcaccgagc tatacgcccg ggtcgacctg ctgcctggcg
    10561 aggacggttc gccggtcctg ctggagctgg agctgtgcga gccgtcgttc ttcctgacca
    10621 gctcaccggg ctcggcggca cgagccgcgg aggcgatcgc cgccgcggtc aggcgctgag
    10681 gaccgacgtc ggtcaggcgg agtcgtcggg gcggtccggg ccgtcgggac ccgacccgcg
    10741 gcgggcgacc acccacgcca ccgctccgac caccacgacc agggccccga tcccgaggac
    10801 ccacggcagc gggctggagt cgctctgctc gacggcttcc tcggcggtgt cctcacgggt
    10861 ggcggtgacg ccgtcgaccg gggccccgct ggtggaagca ggagtcgacc cgggggtcga
    10921 ccccgaggtc ggggtcgacc ccgtcggggt gctcgaggtc gaggccggac cggcgatgtc
    10981 ctgggtgagc gtgaccgtcg ccgacggcga cacggcggtc acccgaacgt tccagacggc
    11041 ggcggacggg aactcgaggc gggcggtgta ctcgcccggg gaccccgccg cggtcatcgg
    11101 gacgggatcg atggccgctc cggcgccgtc gtcggccacg gcggtcaccg tggcgccgtc
    11161 gaccgggtgc ccgtcgcctt cgaaggtgag cttgaccgtg atcgtgctgg atgaccccga
    11221 cggtgtcgcc gacacgacgg cgaggacgcc gtcgtcgccg tgggctcccg ccatcggagc
    11281 gatggcggcg acgcccaccc cgacgacgag caggacggcc aggatcgcgg cgacgaggcg
    11341 gacgagcggc ttctgcatcc ccagatcatg ccccggccgc tgcgacggga ccgagcaccc
    11401 cggccgaact cccgaccgac gtgcgggggc gatcaggcga tcgggacccg gaacgtcgtg
    11461 ctcgccggtg cacccccgac cacgacggtc agctcgaagg tccagtcgcc gggtgtgagc
    11521 tgcacgcctt cggcgacgcc gtggctggcg gtgaccgggg tgatgggaac cgcccgcggc
    11581 gccacgcctc cggtggagac cttcagctcg gccgcgtcga cccgtgccag ggatccgtcg
    11641 cgggccaggt agtagacgtg cacgtcgttg ggaccggccg cggccggcga gacctccatg
    11701 cgaaccgtcg tgtcaccggc ctgcttgacc atctggaccg gctcggcgga tcgggccgcc
    11761 tcccggcccg gcggcgtgcc gaccagtact gcggtgaccg cgaccaccgc caggccgacg
    11821 aggacctcgg cccggtagct gcggagcacc cgggcgctcc agcgggcggc gtcggccagc
    11881 tgtcgtcggt gcacccagcc gaaggccagc agcgccaccg tccccaacgc cttggccagc
    11941 accagccgcc cgtgggtcgt gtcggtgagc gcggagagcg accgctcctg aagcgcggcg
    12001 ttgagcacgc cggtggccac ggtcagcggc gccgcgacca acgccatccg actgaacgcg
    12061 gcagcgcgtt cgcgggtgcc gtcccagctg agcacgagca cgcccagagc accgacccac
    12121 gccgcggcgg ccaggacgtg gagagcgtcg gacgcgacgg ccagtccgac cgggctcgcc
    12181 gtccatgcgt gaccacccag gctgggaagt gccagcgcgg ccaggatccc gatcgcggcc
    12241 agccacggcg cccgccgcaa cgcccggggc gggaggacgg cgagcacgag caccagcgcg
    12301 accagcaccc gcaggcccgc gaccgtgccc gcccagctgg cgccgaggaa ccggccggtg
    12361 gcacccggcc cgtcgaggat cgatccgccg gccaggtcgg ccgcgctggc caggaggctc
    12421 aggccgacgc cgaacaggac cgacaccgct ccgggtagca gcaggaaccg ggccgaggcg
    12481 agctcgctgc cccacccctg gggcccggaa cccgctccgg tgtcggcgtc gccatcagtt
    12541 gggtgctgcc tgcggacccg gggtccgtcg acgaacaggg ccatggccag cacgcctccc
    12601 accagcaggc ccccggtgag cgcgatccac cgcccgaacg cgttcaggac ccggagggcg
    12661 tcgaagccgc cggcggcggc cgcggcggcc gatccggtgc gacgaccgac gtggaacacg
    12721 taggagccgg agatgacgtg gccgtcgtcg gagagggcgc ggtacgacac cgtgtaggtg
    12781 ccctcgggcc cgctcgccac cggctgacgg acctcggtcc cgtccgccgt cgtgctcggc
    12841 gacccctcgg ccacgtcctc acccgccggg tcgagcaccc tgacgccgcc gtcgtcgacg
    12901 ttgaccccgt cgtcgaacgt gagcacgacc tcccgcggcg cctgctccac cacctcgtcg
    12961 ttgccggggg tggaggacac gagcgacgcg tgcgcggacg cggtgcccgc caacggcccg
    13021 gccgcggcca ggaggcccaa cccgacgacc agccagagca gggggcggcg acgggtccgg
    13081 atcacggcgg cgatggtacc caggccctcc gttgaggttg cgccggtccc gaccacgacg
    13141 gcactgtcag tcgtgtccgg gccaccacgg ctcgggcccg cgaccgcccg aacccgacgg
    13201 cgacgaccac cgaggacgta ccccatgacc gacgagccca cccagacccc gcccgcagtc
    13261 gaccgggtca cctacgccgt cgacggcggc gtcgccacga tcaccatcga cgccgcggat
    13321 gcgggcaact ccctgacgtc gtcgatgcgc gacgggatga ccgagctggt cgagtgggcc
    13381 tcggccgacc acggcgtgcg ggcgatcgtg ctgaccggcg ccggggagcg ccacttctgc
    13441 accggcgcca acctgggcgg tccgcagaag ccgactccgc cccgacccga gggcgcgccg
    13501 gaccgcctgc tcggtgacgg cgcccggatg atccggcgcg gctggcagcg gctgatcgcg
    13561 tcgatcctcg actcggagaa gccggtggtc gcccgggtca acggcaccgc cgcgggcgga
    13621 ggcgctcacc tggtgctggc gtgtgacctg gtggtcatgg ccgacaccgc caagctggtc
    13681 gaggtgttcg tccgacgggg catcctcccc gacgcgggcg gcgcctggat gctgcctcgc
    13741 atcgtcgggc cgcaggtcac caaggagctg atgttcctgg ccgacgacgt gtccgccgag
    13801 cggtgccgtg agctgaacat cgccaaccgg gtcgcgcccg ccgccgagct cgacgcggcc
    13861 gtcggcgagc tcaccgggcg cctggtccag gcgccgacca aggcgctcgg gctgaccaag
    13921 tggctggtca accgggcctt cgagtcgagc cgccacacgg ccttcgagga ggaggccgcc
    13981 gcccaggagt ggctgatgca ctcggaggac ttccaggagg gtctggccgc cttccgcgag
    14041 cgtcggtcgc cggagttccg cggctggtga tcgatcgggg gatcgggtcg ggtcggcgac
    14101 cggccgatcc ctttcacagc atttcaacat ctttggtgtt gcgtcaggct gtttgagtcg
    14161 tcacactggc cgccatgtcc gggctcggtt ggatcaggtc agcgtcacca cgtcaagcac
    14221 cgcgcgccgg cgccacctgg gtggctctcg tgcttctcgc cgggctgacg acggcgtgcg
    14281 gggcgggggc ggcggagccg ctccgcccgc tcggcaacgc ctcggcccca cgggccacca
    14341 cccccggggc cgcaaccgac cccacaggga ccccgcccga ctcgatcacg cccaacgctc
    14401 cagagcccgc cggcccgacc acgaccgcac cggagcccgg cgacggcccc ggtcatgtcg
    14461 tcgccgacgt cctcgaccgc tacgaccggg ttctggcccg gctccacgcc gaccccgcag
    14521 cgatgaaccg cccgggcgat cccgtcggct cggaactgct gggagagctg ggccgggtgg
    14581 tgcccgccgg ggccgttctc ttcgacgaca tccggtccca gctggccggc cgggccgccc
    14641 ggggtgagtc gatcccgggc gaggaccgcc agctcccgta cgtgcaccac gccatcgacg
    14701 cggatgtcga gggagccgac cgggtgacct tcacgtggtg cagctggtcg ccgggggtgg
    14761 tgcgcgacac cgccaccggg gcagtcgtcg acgacaccgt cggccacggg accggcacgg
    14821 gcgtggcgga gcggatcgcc gggacgtgga tgctctcgtc gctcgacgag agcactctcg
    14881 acctgctccc ggccggtacg gccgacccct gccccggagc ggatcggagc cggccgtgat
    14941 ctcccctcgg cgtcgccgtc ccgttgccgt cgcggtcgct gtcgcgatcg tgtcggcggt
    15001 gatggctttc ggtggcccgt cgtcggcggg agcggccggg gactccaccg cctcatcgcg
    15061 ggtcacccgc gacgggcgga tcatcacgtc gatcatcaac cggacgccca tccgctaccc
    15121 gggccgggga accgccccca gcacctactg gctcactctc accgacaccg agctgtcgtt
    15181 cctgttggcc gtcctcgcca acgacccccg cctgcagggc gacttcgtcg acgtgatgag
    15241 cacgctggcc gacgccggac cggaccggac cgcggacgtc caggtccaga tccgacgagg
    15301 tcggttcacc gggcagaccc gggccgtgcc caccgaccgg tccgccccgg ctcaggtcct
    15361 ggcccgccgc atgatcacga gcctgccccc gcttcgaccg gtccagagcc ctccgaccgc
    15421 gactccggtc gtgatcggcg agccggcctt cacctcgttc gaccccggga cctgggcgac
    15481 cgtcgtcgac cggtcgctca ccgccggggc cgtcaccgcc cgggtgcggg cccgaccggt
    15541 gtcgttccgg gtccgcagcg gagatcccgc cgacaaccgg atggcgatct gccacgggcc
    15601 gggccggccg ttcgacccga ccgaccccgc cccgccgggc cgccaggccc gccgggccgg
    15661 gacgtgtgcg gtgacctacc tgacccgcac cggggtcgag ggccgccgcg accgctggta
    15721 cggcgagctg accgtgatct ggcgggccga gtggaccacc gacgggatca cgtggcgatc
    15781 cctcggcgac atcccccaga tcgcggtcgt cggtcggacg gtggtgcccg ccaccaccgc
    15841 catcgagtcc cggtcctgac caggcccggg actcgtcagt cgcgtccgcc gaagagcgcg
    15901 gcgccggcgt cgaccaggtc cgggatccgc tggacgaact tctccttcag ctccgcggca
    15961 cgggcggcgt cgaggtggcc gcccgccacg gcagcatcga tctgctcgtc ggcgcccgtc
    16021 acgagggcgt cgctgatctt ctgggcgtcg acgccgtgct cggacgcgag ctgatcgagc
    16081 gtcttgccgg ccttcagctc gtcggcgagc tgctctccgg tcaggccgaa caccgacgcg
    16141 gccgcgtcga gcatctcctg gcgtcgggcg tcccaggcat cccgccgttc ggaccgggcc
    16201 gcctccgctc gctgcgtcac ctcggcggtg accgcatcgg cctgcgcctg ggtgaggacg
    16261 ccgttgtgca ccagggcggc cagggcctcg tcgaggaggc ggtgcgagcc gccgtggtcg
    16321 cgtccagccg actcggccgg ggccgcgtcg gcatcgggcg acgggggcgt cgtcggagcg
    16381 ctc
//
LOCUS       DAOQOD010000058        16196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1276_length_16196_cov_10.358776, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000058 DAOQOD010000000
VERSION     DAOQOD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_16196_cov_10.358776"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..909
                     /gene="truA"
                     /locus_tag="PLZ72_07520"
     CDS             88..909
                     /gene="truA"
                     /locus_tag="PLZ72_07520"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNA36178.1"
                     /translation="MSDADTTVRLRLDVAYDGSGFHGFAENRDVVTVGGALRNALERV
                     LSVPVAITCAGRTDAGVHARGQVVTLDVPVAAIDALARRDRRGPGDGYCRLRDALNAL
                     VGPSVVVTAVSPATDDFDARFSARSRTYRYTVWSSEVPDPFLAASAWWVDQPLDLDAM
                     RTASEQVVGEHDFSCFCRRPRATDPGAEPVSLVRRVLSARWDESPDRPGLLTFTISAT
                     AFCHQMVRSITGMVVDVGRGRRTPDDVTAALASLDRARAGQLAPPHGLVLWEVGY"
     gene            1239..1688
                     /gene="rplM"
                     /locus_tag="PLZ72_07525"
     CDS             1239..1688
                     /gene="rplM"
                     /locus_tag="PLZ72_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNA36179.1"
                     /translation="MRTYSPRADEIERDWYVVDAEGLTLGRMSTEVARVLRGKHKAMY
                     TPHMDTGDHVIIINADKVVLTNNKAEAKQVYRHTGYPGGIRSTTYATLLAEKPAEAVR
                     RTVRGMLPKNRLGRQMLKKLKVYAGPTHPHSAQQPQPLAIEHARARA"
     gene            1718..2110
                     /gene="rpsI"
                     /locus_tag="PLZ72_07530"
     CDS             1718..2110
                     /gene="rpsI"
                     /locus_tag="PLZ72_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNA36180.1"
                     /translation="MSTPLTQTTGRRKRAVARVRLRPGTGVITINKRPLEDYFPNATH
                     RMILTEPLRVTETSETYDVDATLHGGGVTGQAGAIRLGIARSLIEVDPELRPALKKAG
                     FLSRDAREKESKKYGLKKARKAPQYSKR"
     gene            2121..3494
                     /gene="glmM"
                     /locus_tag="PLZ72_07535"
     CDS             2121..3494
                     /gene="glmM"
                     /locus_tag="PLZ72_07535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNA36181.1"
                     /translation="MTVRFGTDGVRGLAGRDLTPEIATAIGRAAVRAFGARRVVIGRD
                     TRRSGPLLEAALAAGVCAEGADAVLAGVVPTPAVAFAAERDGVVGVVISASHNAFADN
                     GIKLFAPGGLKLTDDQQHAVEAVIAAALDAVATAGTPYEGVPVGADVGSLLADVTLAE
                     EYAASIEGVTEGRRLDGLRVVVDCANGAASAVAPDVLADLGAEVTVLFAEPDGTNIND
                     GCGSTHPEVLQGAVLETGAHLGLAFDGDADRLLAVDERGRLVDGDQIMALCATDLRDR
                     GRLTDDTVVVTVMSNLGFRQAMGREGITVIETAVGDRHVLAAMAEGDLSLGGEQSGHL
                     VFGDLGTTGDGLLSAVVLADLVVREGVLGGEVTVGAMVDAAMTKLPQVLRNVAVDAPM
                     PDVADRLADEIDRVGAELGDEGRVLLRPSGTEPVVRVMAEAPTEDAAAAAVDRLVAAV
                     EALRTSA"
     gene            3599..7108
                     /locus_tag="PLZ72_07540"
     CDS             3599..7108
                     /locus_tag="PLZ72_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNA36182.1"
                     /translation="MCGIIAVVRRPTDRTPAAAAELAQVLHDVAPLLEASDPGSTQDR
                     TAALSAAAERLTTVDAALRGVPGVVSLRSDRAAADRLAAAADGLAERLAQLEAELDDP
                     QTAPPAEQLEAVNAALLACKDALWAIRQDRLRAVREVDGLLGATFDADGGTEAGVAVA
                     LSLHQALSALDRLEVRGRDSAGLEVQLTGHGLDPADPAVSRALEERAGVSFTDGSVRL
                     GDGVLVVVHKAAAEIGELGDNTAALRAAIRSDDLLQRALRSADVDGLVLGHTRWASIG
                     IISEPNAHPQVSDELDAEGTALEPGAGGAPCVTAVLNGDVDNHADLVASDGLRLPAEV
                     TTDAKVIPTLVSRGLVAGRAPREAFREAVARLEGSVAIGAASAASPDRLLLALRGSGQ
                     ALYVGLADEAYIVASEPYGVVEETATYLRLDGETPADPDNPVASRGQIVELAAGRAGT
                     IDGIGRWSYDGTELPVTAADLTHAQITTRDIDRGTFPHFLLKEITDAPGSFRKTLRGK
                     LIESDGRFDVELPEDSLPAEVRRRLAAGEVTRVLVIGQGTAAVAGQSLAEHLRELLAD
                     TEVRVEALPATELSGFRLRADMSDTLAVAISQSGTTTDTNRTVDLLRGRGASVVAIVN
                     RRNSDLTDKADGVLYTSDGRDVEMSVASTKAFYAQVAAGLLLGYSISEQVPGGRTPDH
                     PDEQELLRSLAQVPAALEATLATRPDIAAAARTFAPSRRYWAIVGNGPNQIAARELRI
                     KLSELCYKSIACDATEDKKHIDLSSEPMILVCAAGLVGSTADDVAKEVAIFRAHKAAP
                     IVIASNGDRAFDAALAVLSVPDTHPRLGFVLATMVGHLFGYEAALGIDAQARPLREVR
                     AAIDEAVSTLGGDLRELNDGEALMRRLRPVLTASASAFMDGLRTGSYDGHLEAGTAVR
                     VAGLFRYALGISPLDAYQVEFGKVGTPGVVLEDLEAGLTAAIEELTRPVDAIKHQAKT
                     VTVGISRSDESLLQVALVDALIQAGSPRDRLTYSTLRSLGELDPAVIEVVGSTRYAVE
                     HGDDLDQAQLVVLDRRGISTGLASRVDRDSRLRGTKALVAREQELMVARGRADGRLVV
                     IVPETKDGVTTGLQLLHVVLPDTLPAPTARAVLQGYRRRYQALKDAVTETEDVFRDDL
                     LAQQPVADLLTVPILDLADRWRT"
     gene            7105..7527
                     /locus_tag="PLZ72_07545"
     CDS             7105..7527
                     /locus_tag="PLZ72_07545"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNA36183.1"
                     /translation="MTDRDDAPTSVGAAGVGTVVGVGTDLVAVDRLRTALDRTPSLLH
                     RLFTPAEQARCGRHADPLPHLAARFAAKEATMKALGRGMSAMSFTDVEVLTAADGSPS
                     LRLTGRARQVADTVGVTSWLVSLTHDGPMAHAVVLART"
     gene            7628..9016
                     /locus_tag="PLZ72_07550"
     CDS             7628..9016
                     /locus_tag="PLZ72_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNA36184.1"
                     /translation="MLTVLTPSQMAAVDAAAAEPVEVLIGRAAGAVARAAVDLMGGTY
                     GRRVVVVAGPGHNGEDGRVAAALLARRGVRVTVHDVADAPTVLGPADLVIDAAFGTGF
                     RGNYRFPDVDGAPVLAVDIPSGISGLTGAVSGAPAPAIRTVTFAALKPGLVLGAGPSY
                     AGEVVVADIGLDVEDTAVVGAAGCHLVTDDDVLAWVPARAVDDHKWRHAVWVVAGSPG
                     MTGAAQLCSTAALRAGAGYVRLTVPGVEDPPAPTEAVVLGARTDDWDRLMSSDASRFG
                     CVAIGPGLGRDAGSAARVRAALAATDRPVVLDGDGLAAIADAPTAALHSRPVVDDVPA
                     PTVLTPHDGEFEMLTGAPPGEDRVDAARALAATTGAVVLLKGPTTVVAEPDGDVLLVR
                     SGDERLATAGTGDVLTGTIAALIASGALPVRAAAAGAHLHGRAAELGPRRGLVASDVA
                     DALPDAWADLLG"
     gene            9123..10316
                     /gene="alr"
                     /locus_tag="PLZ72_07555"
     CDS             9123..10316
                     /gene="alr"
                     /locus_tag="PLZ72_07555"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNA36185.1"
                     /translation="MPRHAAVHVDLDAVAHNVAVLKDLVAPAELCVVVKADGYGHGSE
                     DVAAVAVAAGADRLAVALVEEGQELRDVGIDAPVLVLSQPPPDAMVDAFAAGLNPTVD
                     TAEGIAAAAAAAGGTPWPVQLKVDTGMHRVGCDLADAVDLARAVLDAGLELQGTFTHL
                     AVADEPDRPETDEQLELFAAVLAELAAAGIDPGLRHAANSAAALVHPAARLDLVRVGI
                     ATYGVPPSADLELPVPLEPAMNVRAEVSRVRSVAAGEGVSYGLRHRFRSPARLAVVPL
                     GYADGVPRRLGELGADVLIGGVRRPMRGVVTMDQLVVEVTDGPEVAVGDEVVLIGRQG
                     DEEITTGEWAEMLGTISYEIVCGFGPRLPRRHLTRISHGAGFVDGWADRPAGPEPDPS
                     GGGGS"
     gene            10313..11254
                     /locus_tag="PLZ72_07560"
     CDS             10313..11254
                     /locus_tag="PLZ72_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNA36186.1"
                     /translation="MIDGIDGVAVGHWSDPVALTGCTVVVFDPAVVASGEVRGGAPAS
                     HEFALLDPAATVPTIDAVVLSGGSAFGLAAAVGVTGVLEEHDRGLPTMAGRVPIVVGL
                     SLFDLAVGDASVRPGVEQGRIAAAEAIAGAGAGSAVGRIGTGTGATASKWRGIERAVP
                     GGLVGALRRDGDLVVAALVAANPWGDVHGAGYDDEPWPELPPPGDTGLERTADGTAAA
                     GSGAVTNTTIGVVVTNARLDKLGCHHVARGAHDGLARAITPAHSRVDGDAFVAAATGA
                     VDATVERVRAHAVEVVDRAIRQVAELADLAPGDRLVP"
     gene            11339..12010
                     /locus_tag="PLZ72_07565"
     CDS             11339..12010
                     /locus_tag="PLZ72_07565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNA36187.1"
                     /translation="MAPVADDSPVPADRAERAAAFAELRDRALECRRCRLAEGRTQVV
                     FGSGDPDADLMFVGEGPGAEEDRQGLPFVGRSGQLLDRLMAEEMDLTRDRAYIANVVK
                     CRPPDNRDPKLDEIEACRPWLEAQVDLIGPKVIVTLGNFSSRLLLDTREGITRLRGSV
                     YPYRDRPDGTPVQLVPTFHPAAVLRGGGEPMAGMRADLVRAKQTLGTAASAGTGASVD
                     AGGSA"
     gene            12007..12678
                     /gene="tsaE"
                     /locus_tag="PLZ72_07570"
     CDS             12007..12678
                     /gene="tsaE"
                     /locus_tag="PLZ72_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNA36188.1"
                     /translation="MIDQARLVARTASVLETRDLAAALAELARPSDLLVLVGDLGAGK
                     TAFTQGFGRGLGIDEQITSPTFALVRSYTGRLDLYHLDVYRLEQVSEALDLGLSELLD
                     DGSVTVIEWGDTIAPALPHDYLEVHLRFDVPDGPAPTGESSAPATEAAAGAAAGSAEV
                     FERRIVELRPIGPRWSARVRALGAALAPWTEGGAGNDADTGTGNDADTGERGAPTERD
                     GVGEC"
     gene            12672..13370
                     /gene="tsaB"
                     /locus_tag="PLZ72_07575"
     CDS             12672..13370
                     /gene="tsaB"
                     /locus_tag="PLZ72_07575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNA36189.1"
                     /translation="MLILGIETATVKAGCAIGGHEGVLASAHSGKAKRHAENLTPAIE
                     FICGQAGIELGEIGLVAVDVGPGLFTGLRVGVATAKAIAFGLRVPMIGVSSLDLLAFP
                     VRFSNRLIVTAIDARRGELYYAFYRQVPGGVQRLGEHQIGSPDDLASQLLASPDEILL
                     VGDGANRYAEAFEGLAKVELVTHDHCHPSAESLVQLAHARALREEFERPDAIEPIYLR
                     RPDAEINWSVRDAR"
     gene            13378..13956
                     /gene="rimI"
                     /locus_tag="PLZ72_07580"
     CDS             13378..13956
                     /gene="rimI"
                     /locus_tag="PLZ72_07580"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNA36190.1"
                     /translation="MLPTHRGPSVVAPAGDGVVVVPMRRRHLRGVVDIENRTNHRPWS
                     LSLFQGELRMPTSRRYVVALDGHRVVGFAGLMLTGFEGHITNVAVDPDHRRRHIATRM
                     LLVLFREALALDVEDLTLEVRVTNRGAQDLYRGFGFSPGGVRPNYYNDIKEDALIMWA
                     HGIGTDEGRARLDALTDALPVALAELVVDRAG"
     gene            13946..15001
                     /gene="tsaD"
                     /locus_tag="PLZ72_07585"
     CDS             13946..15001
                     /gene="tsaD"
                     /locus_tag="PLZ72_07585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNA36191.1"
                     /translation="MPADAASPRLLGGAAAGSDALVLGIETSCDETAAACVRGGTDVL
                     SSVVSSQVDLHAKYGGVVPEIASRAHNELIIPVVARAMVEAGVDGRRIDAVAATTGPG
                     LIGALLVGVSAAKALALTWDVPFVSVNHLEAHLYAALLEDPHLELPMVVLLVSGGHTM
                     LVLMEDHGRYRILGQTLDDAAGEAFDKVARFLGLGYPGGPAIDREAMDGDPEAVRFPR
                     SMIDDGLDFSFSGLKTAVVNHVRAKPDVSTPDVAASFQAAVVDVLVAKARRAAAETGA
                     RALAIGGGVAANSLLREQFLTACTDDGLHAFLPSRAMCTDNAAMIASAGWYRLRDDGP
                     SPLSTGADPNLRLTSHR"
     gene            15015..>16196
                     /locus_tag="PLZ72_07590"
     CDS             15015..>16196
                     /locus_tag="PLZ72_07590"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HNA36192.1"
                     /translation="MGEASSGAGEGAVAGERAGTNEWAGADEPAGPDEWSGRFEQQAA
                     ACDDLGSPLWARLLRIVAADVRAEGPSWSVLRERAGLRFGQAGPLRLVGAAHRLALAG
                     SAPEWASLLPSCGGEAPPADADGERVLRSGWAGLVDHHGDALVAGLDREVQTNEVGRA
                     AALGYAHAVALADRGGPARLVEIGCSGGLNLRLDRFGLDLSGGGSGPVLGDPDGRVRL
                     APEMSTVARAGLSTVGVVERTGIDPHPIDPTSVEGRLTLLSFVWPDQVERFARLAAAI
                     DLAREVPAELVATAGRPTAEVLAEVLARPVGAGTATIVQHSIVWQYIPTDERAAVTSA
                     IESAGERATAGEPVVWVRFEPDVWDRGRVAVWVRRWPEGGDRLVAHADYADAAWVVPG
                     VA"
BASE COUNT         2051 a         5994 c         5947 g         2204 t
ORIGIN      
        1 cctcaccccg accccaccct cggtttctga cgacaagtca cgacaaccgc ggtactcaaa
       61 tgtcatgaga acccgcggga accgggcgtg agcgacgccg acacgaccgt tcggctgcgg
      121 ctcgacgtcg cgtacgacgg gtccggcttc cacggcttcg ccgagaaccg ggacgtggtc
      181 accgtcggcg gcgccctgcg caacgctctg gagcgggtgc tgagcgttcc ggtcgcgatc
      241 acctgcgccg gccgcaccga cgcgggggtg cacgcccgcg gtcaggtggt gaccctcgac
      301 gtcccggtcg ctgcgatcga cgctctggcc cggcgagacc gacggggtcc cggcgacggc
      361 tactgccggc tgcgcgacgc gctgaacgcg ctggtcggtc cgtccgtggt ggtgaccgcc
      421 gtgtcgcccg cgaccgacga cttcgacgcc cgcttctcgg cccggtcccg cacgtaccgc
      481 tacaccgtgt ggagctctga ggtccccgac ccgttcctgg ccgcctcggc atggtgggtc
      541 gatcagccgc tcgacctgga cgccatgcgg accgcgagcg agcaggtcgt gggggagcac
      601 gacttctcgt gcttctgccg acggccccgg gccaccgatc cgggcgcgga gccggtgtcg
      661 ctggtgcgga gggtgctctc ggcccgctgg gacgagtcgc cggaccgccc gggtctgctc
      721 accttcacga tcagcgcgac ggcgttctgc caccagatgg tccgatcgat caccgggatg
      781 gtcgtcgacg tcggccgggg tcgacgcacg cccgacgacg tcaccgccgc gctcgcctcg
      841 ctcgaccggg cccgggccgg ccagctcgcc ccaccccacg gtctggtcct gtgggaggtc
      901 ggctactgag gatgcacccg ccgccgtcgt tgttgtagct ggtggtcgct ggggtggcgt
      961 gtgggctaca agttcggcgg cggctggcgt tgttgtagct ggtggtcgct ggggtgacgt
     1021 gtgggctaca agttcggtgg caggcgctgg ggctgccccg gggccgcttg caccctccga
     1081 cgcccagccc gtacgcttgc ccgtcggccc gagcggtcgc cggtatcgcg ggtgcccgtc
     1141 ggccccgcag tccgggaccc gcccgttgga ccacaccccc acgtcggggt cgtccgccac
     1201 ccggccggtt gagatcgaag cagaagagag tgagcgctgt gcgcacctat tcgccacgag
     1261 cagacgagat cgaacgcgac tggtacgtcg tcgacgccga ggggctgacc ctcggtcgca
     1321 tgtccaccga ggtggcccgg gtccttcggg gcaagcacaa ggccatgtac accccgcaca
     1381 tggacaccgg cgaccacgtg atcatcatca acgccgacaa ggtcgtgctg accaacaaca
     1441 aggccgaggc caagcaggtc taccgccaca ccggctatcc gggcggcatc cgctccacca
     1501 cctacgccac gctgctggcc gagaagcccg ccgaggccgt ccgccgcacg gtgcggggca
     1561 tgctccccaa gaaccgactc ggccgccaga tgctgaagaa gctgaaggtg tacgccggac
     1621 cgacccaccc tcattcggcc cagcagccgc agcccctggc catcgagcac gcccgcgccc
     1681 gggcctgaga cgcgtcgagc acggagatca agcagagatg agcacccctc tcacccagac
     1741 caccggccgt cgcaagcggg ccgtcgcccg cgtccggctg cggcccggca ccggcgtgat
     1801 caccatcaac aagcgcccgc tcgaggacta cttcccgaac gcgacgcacc ggatgatcct
     1861 gaccgagccg cttcgggtca ccgagaccag cgagacctac gacgtcgacg ccaccctcca
     1921 cggtggcggc gtgaccggcc aggccggcgc catccgtctg ggaatcgccc gctcgctcat
     1981 cgaggtcgac cccgagctgc gccccgccct caagaaggcc ggcttcctgt cccgcgacgc
     2041 acgcgagaag gaatccaaga agtacggcct caagaaggcc cgcaaggccc cgcagtactc
     2101 caagcgctga tcgtcccccg gtgaccgtcc gcttcggcac cgacggggtg cgcgggctcg
     2161 ccggtcgcga cctcaccccc gagatcgcca cggccatcgg ccgggccgcg gtccgggcct
     2221 tcggtgcccg gcgcgtcgtc atcggccgcg acacccgccg gtcgggtccg ctgctcgagg
     2281 ccgccctggc cgccggggtg tgcgccgagg gtgccgatgc ggttcttgcc ggcgtcgtgc
     2341 ccacccccgc ggtggcgttc gccgccgagc gcgacggtgt ggtcggggtg gtcatctcgg
     2401 cgtcgcacaa cgccttcgcc gacaacggca tcaagctgtt cgcccccggc gggctcaagc
     2461 tgaccgacga ccagcagcac gccgtcgagg cggtgatcgc cgcggcgctc gacgcggtag
     2521 cgaccgccgg aaccccgtac gagggcgtcc cggtcggagc cgacgtcggg tccctgctcg
     2581 cggacgtcac cctggccgag gagtacgcgg cgtcgatcga aggcgtcacc gagggtcgtc
     2641 ggctcgacgg gctgcgggtg gtcgtcgact gcgcgaacgg cgccgcgtcg gccgtcgccc
     2701 ccgacgtgct cgcggacctc ggcgccgagg tcaccgtgct gttcgccgag cccgacggca
     2761 ccaacatcaa cgacggctgc ggctccaccc accccgaggt gctccagggc gcggtgctcg
     2821 agacgggcgc ccatctgggg ctcgccttcg acggcgacgc ggaccgactc ctggcggtcg
     2881 acgagcgggg ccgcctggtc gacggcgacc agatcatggc gctgtgcgcc accgacctgc
     2941 gcgaccgcgg gcggctcacc gacgacaccg tcgtggtgac cgtcatgtcc aacctgggct
     3001 tccgtcaggc gatgggacgc gagggcatca cggtgatcga gaccgcggtg ggtgatcgcc
     3061 acgtgctggc ggccatggcc gagggcgacc tcagcctggg gggcgagcag tcgggccacc
     3121 tggtgttcgg cgacctgggc acgaccgggg acggtctgct gagcgcggtg gtgctggccg
     3181 acctggtggt ccgcgagggc gtgctgggcg gcgaggtcac cgtcggggcg atggtcgacg
     3241 cggccatgac caagctgccg caggtgcttc gcaacgtcgc ggtcgacgcc ccgatgcccg
     3301 acgtggccga ccgcctggcc gacgagatcg accgggtcgg cgcggagctc ggcgacgagg
     3361 gccgggtcct gctgcggcca tcgggcaccg agcccgtcgt gcgggtcatg gccgaggcgc
     3421 ccaccgagga cgctgccgcg gccgcggtcg accgactggt cgcggcggtc gaggccctgc
     3481 gtacctcggc ctgaccggtc cgtcggtccg gacccgctcc tggggatcgg gtgatcgact
     3541 cggtgcggaa atcgggcacc ccctacactt gaccgacccc ctacggaggt gcaccaaggt
     3601 gtgtgggatc atcgccgtcg tccggcgacc gaccgatagg acgccggccg cggcagcgga
     3661 gctggcccag gtcctccacg acgtcgcgcc gttgctcgag gcgtccgacc cgggatcgac
     3721 gcaggatcga acggccgcac tgtccgccgc cgccgagcgg ttgacgacgg tcgacgctgc
     3781 gctgcgcggc gtccccggtg ttgtgagcct ccgctcggac cgtgccgccg cggaccgact
     3841 ggctgcggct gccgacggcc tcgccgagcg gctcgcccag ctcgaggccg agctcgacga
     3901 cccccagacc gcaccgcccg ccgagcagct cgaagcggtg aacgccgcgc tgctcgcctg
     3961 caaggacgcg ctgtgggcca tccgccagga ccggctccgt gccgtccgcg aggtcgacgg
     4021 gctcctggga gcgactttcg acgccgacgg cggcaccgag gccggggtcg cggtagcgct
     4081 cagcctccac caggcgctgt cggcgctcga ccgcctcgag gtccgcggcc gcgactccgc
     4141 cgggctggag gttcagctga ccggccacgg gttggacccg gccgacccgg ccgtgagccg
     4201 agccctcgag gagcgcgccg gcgtgtcgtt caccgacgga tcggtccgac tcggtgacgg
     4261 tgtcctggtc gtggtccaca aggccgcggc cgagatcggg gagctgggcg acaacaccgc
     4321 ggcgctgcgc gccgcgatcc gctccgacga cctgctgcaa cgcgctctgc gctccgcgga
     4381 cgtcgacggc ctggtgctcg gccacacccg gtgggcgtcg atcggcatca tctccgaacc
     4441 caacgcccac ccccaggtgt ccgacgagct cgacgccgag ggcacggcgc tcgaacccgg
     4501 cgccggcggc gccccgtgcg tgaccgcggt cctcaacggc gacgtcgaca accacgcgga
     4561 cctggtcgcg tcggacggac tgcgcctccc cgccgaggtc accaccgacg ccaaggtgat
     4621 ccccaccctg gtcagccggg gtctcgtcgc cgggagggca ccccgtgagg cgttccgcga
     4681 ggcggtcgca cgcctggagg ggtcggtggc gatcggcgcg gcgtccgccg cgtccccgga
     4741 ccgactgctc ctggcgctgc gcggcagcgg tcaggccctc tacgtcgggt tggccgacga
     4801 ggcctacatc gtcgcgtccg agccctacgg cgtggtcgag gagaccgcca cctacctccg
     4861 cctcgacggc gagacccccg cggaccccga caacccggtc gcgagccggg gccagatcgt
     4921 cgagctggcc gccggacggg ccggcacgat cgacgggatc gggcgctggt cctacgacgg
     4981 caccgaactg ccggtgaccg cagccgacct gacccacgcc cagatcacca cccgcgacat
     5041 cgaccgcggg accttcccgc acttcctgct caaggagatc accgacgcgc ccggctcgtt
     5101 ccgcaagacg ttgcggggca agctcatcga gtcggacggc cggttcgacg tcgagctgcc
     5161 cgaggactcg ctgcccgccg aggtccgtcg acggctggcc gccggcgagg tgacccgggt
     5221 gctcgtgatc gggcagggca ccgcggcggt cgccggccag agcctggccg aacacctccg
     5281 cgagcttctg gccgacaccg aggtccgcgt cgaggcgcta ccggcgacgg agctgtcggg
     5341 attccgcctc cgcgccgaca tgtccgacac gttggccgtc gcgatcagcc agtcgggcac
     5401 caccaccgac accaaccgca ccgtcgacct gcttcggggg cggggagcgt cggtggtggc
     5461 gatcgtcaac cgacgcaact cggacctgac cgacaaggcc gacggcgtcc tctacacctc
     5521 cgacggacgc gacgtggaga tgagcgtggc gtcgaccaag gcgttctacg cgcaggtcgc
     5581 ggccggactg ctgctcggct actccatctc cgagcaggtg cccggcggcc gcacccccga
     5641 tcaccccgac gagcaggagc tgctgcgctc cctcgcccag gtgcccgccg cgctcgaggc
     5701 caccctcgcc acccgccccg acatcgcggc cgcggcccgg accttcgccc cgtcgcgccg
     5761 ctactgggcg atcgtcggca acggccccaa ccagatcgcg gcccgcgagc tgcggatcaa
     5821 gctgtcggag ctctgctaca agtcgatcgc gtgcgacgcc accgaggaca agaagcacat
     5881 cgacctgtcc tccgaaccga tgatcctggt gtgcgccgca ggcctggtcg gatcgaccgc
     5941 cgatgacgtg gccaaggagg tggcgatctt ccgggcccac aaggccgccc cgatcgtcat
     6001 cgcctcgaac ggcgaccgcg ccttcgacgc cgcgctggcg gtgctcagcg tgcccgacac
     6061 ccacccccgc ctggggttcg tgctggccac catggtcggg cacctgttcg gctacgaggc
     6121 cgcgctcggc atcgacgccc aggcccggcc gctgcgcgag gtgcgcgccg cgatcgacga
     6181 ggcggtctcc accctcggcg gtgacctgcg ggagctgaac gacggcgagg cgctgatgcg
     6241 tcggctccga ccggtcctga ccgcgtcggc ctcggcgttc atggacggcc ttcgcaccgg
     6301 ttcctacgac gggcacctcg aggccggcac cgcggtgcgg gtcgcggggc tgttccgcta
     6361 cgcgctgggc atctctccgc tcgacgccta ccaggtcgag ttcggcaagg tcggcacccc
     6421 gggagtcgtg ctcgaggacc tcgaagcggg tctgaccgcg gcgatcgagg agctcacccg
     6481 gcccgtcgac gccatcaagc accaggccaa gaccgtcacc gtcggcatca gccgctccga
     6541 tgagtcgctg cttcaggtcg cgctggtcga cgctctcatc caggccggtt ctccccgcga
     6601 ccgcctcacg tactccacgc tccgctccct cggcgagctc gacccggcgg tcatcgaggt
     6661 cgtcggctcc acccgctacg ccgtcgagca cggcgacgac ctggaccagg cccagctggt
     6721 ggtgctcgac cgtcggggga tctccaccgg gctggcgtcg cgggtcgacc gcgacagccg
     6781 gctgcgcggc accaaggccc tggtcgcccg cgagcaggag ctgatggtgg ctcggggccg
     6841 ggccgacggc cggttggtcg tcatcgtccc cgagaccaag gacggcgtga ccaccggcct
     6901 gcagctgctg cacgtggtgc tgcccgacac gttgcccgcg ccgaccgccc gcgcggtgct
     6961 ccagggctac cgccgtcgct accaggcgct caaggacgcg gtcaccgaga ccgaggacgt
     7021 gttccgcgac gacctgctgg cccagcagcc ggtggccgac ctgctcacgg tcccgatcct
     7081 ggacctggcc gaccgctggc ggacgtgacc gaccgggacg acgccccgac gtcggtcggc
     7141 gccgcggggg tcgggacggt cgtgggcgtc ggcaccgacc tggtcgccgt cgaccggttg
     7201 cgcaccgcgc tggatcggac gccgagcctg ctccaccgcc tgttcacccc ggccgagcag
     7261 gcccgctgcg gtcgacacgc cgatccgctc ccgcacctgg ccgcccgctt cgcggccaag
     7321 gaagcgacga tgaaggccct cggtcgtggc atgtcggcca tgtcgttcac cgacgtcgag
     7381 gtgctgaccg cggcggacgg ctcgccgtcg ctgcgcctca ccggtcgggc ccgccaggtg
     7441 gccgacacgg tgggggtgac cagctggctg gtgtcgctca cccacgacgg tccgatggcc
     7501 cacgccgtcg tgctggcccg gacctgaccg tcccggcggg gtccgtcccg agggcaccgt
     7561 ccggtcgggg atcccggcgc cgtcgatctc ggccccatcc ggtggcgggt cgcggcagga
     7621 tgacggtgtg ctcaccgtcc tgaccccgtc gcagatggcc gcggtcgacg ccgcggccgc
     7681 cgagccggtg gaggtgctca tcgggcgggc cgccggcgcc gtggcccggg cagcggtcga
     7741 cctgatgggc gggacctacg gacgtcgggt cgtggtcgtg gccggaccgg gccacaacgg
     7801 cgaggacggt cgggtcgccg ccgcgctcct ggcccggcgg ggggtgcggg tgaccgtgca
     7861 cgacgtggct gacgccccca cggtgctcgg accggccgac ctggtgatcg acgccgcctt
     7921 cggaaccggg ttccggggca actaccggtt ccccgacgtc gacggtgctc cggtcctggc
     7981 cgtcgacatc ccgtcgggga tctcgggcct gaccggcgcc gtctcgggcg ctcccgctcc
     8041 cgcgattcgc accgtcacct tcgctgcgct caagccgggc ctcgtgctcg gagccggacc
     8101 ctcctacgcc ggcgaggtcg tcgtggccga catcggcctc gacgtcgagg acaccgccgt
     8161 ggtcggcgcc gccggctgtc acctggtcac cgacgacgac gtgctcgcct gggtcccggc
     8221 ccgagccgtc gacgaccaca agtggcgtca cgccgtgtgg gtggtcgccg ggtcgccggg
     8281 catgaccggg gccgcgcagc tgtgctcgac cgccgcgctg cgtgccggag ccggctacgt
     8341 gcggttgacc gtccccggcg tcgaggaccc gcccgccccg accgaggcgg tggtgctcgg
     8401 ggcgcgaacc gacgactggg accgcctgat gtcgagcgac gcctcccggt tcggctgcgt
     8461 cgccatcggg cccggactgg gtcgcgatgc cgggtccgca gcgcgggttc gcgccgcgct
     8521 ggccgcgacg gaccggccgg tcgtcctcga cggcgacggc ctggccgcga tcgccgacgc
     8581 cccgacagcg gcactccact cccgtcccgt cgtggacgac gtgccggcgc cgaccgtgct
     8641 caccccccac gacggcgagt tcgagatgct gaccggggcg ccccctggcg aggaccgcgt
     8701 cgacgcggcc cgcgccctgg ccgccaccac cggggcggtg gtgctgctga agggaccgac
     8761 gaccgtggtc gccgagcccg acggcgacgt gctgttggtc cgatccggag acgagcgtct
     8821 ggccaccgcc gggaccggcg acgtcctcac cggcacgatc gccgcgctga tcgcgtcggg
     8881 ggcgctgccg gttcgggccg cggccgcagg cgcgcacctg cacggtcgcg ccgccgagct
     8941 cggtccgcgg cgcggcctgg tcgcctccga cgtcgccgac gccctgcccg acgcctgggc
     9001 cgacctgctc ggctgacacc ccccactctc gctttgtccc gcagggacgc ggtgatcacc
     9061 gcgtccctgc gggacagagc ggagccgggg gtgggcgggg cggcgggtag catcgacctg
     9121 tcatgcctcg tcacgccgct gtccacgtcg acctggacgc cgtggcgcac aacgtggcgg
     9181 tcctcaagga tctggtcgcc cccgccgagc tgtgcgtcgt ggtcaaggcc gacggctacg
     9241 gccacggctc cgaggacgtc gccgcggtcg cggtggcggc cggggcggac cgcctggccg
     9301 tcgctctggt cgaagagggc caggagctgc gcgacgtcgg catcgacgcg ccggtcctgg
     9361 tgctgtccca accgccgccg gatgccatgg tcgacgcctt cgccgccggt ctgaacccca
     9421 cggtcgacac cgccgagggg atcgccgctg ccgcggccgc ggccggcggg acgccgtggc
     9481 cggttcagct caaggtcgac accggcatgc accgcgtcgg ctgcgacctc gccgacgcgg
     9541 tcgacctggc ccgcgccgtg ctcgacgccg gactggagct ccagggcacg ttcacccacc
     9601 tggctgtcgc cgatgagccc gaccgccccg agaccgacga gcagctcgag ctcttcgccg
     9661 cggtgctcgc cgagctcgcc gcggcgggga tcgaccccgg gctccgccac gccgccaact
     9721 ccgctgctgc gctcgtacac cccgcggcgc ggctggacct ggtgcgcgtc gggatcgcca
     9781 cctacggggt gcccccgtcg gccgacctgg agctcccggt cccgttggag cccgccatga
     9841 acgtgcgcgc cgaggtgtcg agggtgcgca gcgtcgccgc cggcgagggc gtcagctacg
     9901 gcctgcgtca ccgcttccgg agcccggccc gtctggcggt cgtgccgctc ggctacgcgg
     9961 acggcgttcc gcgtcggctc ggtgagctgg gcgccgacgt gctcatcggt ggcgtgcgtc
    10021 ggcccatgcg cggcgtggtg accatggacc agctggtcgt cgaggtgacc gacgggcccg
    10081 aggtggccgt cggcgacgag gtggtcctca tcggtcgcca gggcgacgag gagatcacca
    10141 ccggcgagtg ggcggagatg ctcggcacca tctcctacga gatcgtctgc ggtttcggcc
    10201 cgaggttgcc gcggcgacac ctgacccgga tcagccacgg agccggcttc gtcgacgggt
    10261 gggcggaccg gcccgccggg cccgaacccg atccatccgg cgggggtggg tcgtgatcga
    10321 cgggatcgac ggggtggcgg tcgggcactg gagcgacccg gtggcactca ccggctgcac
    10381 cgtcgtggtg ttcgacccgg cggtggtcgc ctcgggcgag gtgcgcggcg gggcgccggc
    10441 cagccacgag ttcgccctgc tcgacccggc ggcgacggtg ccgacgatcg acgccgtggt
    10501 cctcagcggg ggttctgcgt tcggcctggc cgccgcggtc ggcgtcaccg gtgtcctaga
    10561 ggagcacgac cggggtctgc cgaccatggc gggccgggtg ccgatcgtgg tcggtctcag
    10621 cctgttcgac ctggccgtcg gcgacgcctc ggtccgtccc ggggtcgagc agggtcggat
    10681 cgcggccgcc gaggcgatcg ccggtgcggg ggcgggttcg gccgtgggcc ggatcggtac
    10741 cggcaccggg gcgaccgcgt ccaagtggag gggcatcgag cgcgctgttc ccggcgggct
    10801 ggtcggtgcg ctgcggcgcg acggcgacct ggtcgtcgcc gctctcgtcg ccgccaaccc
    10861 ctggggcgac gtccacggcg ccggctacga cgacgagccg tggccggagc tgccgccgcc
    10921 gggtgacacc ggcctggagc gcaccgccga cggcacggcc gccgccggct cgggcgccgt
    10981 caccaacacg acgatcggtg tcgtggtgac caacgcgagg ctggacaagc tgggttgcca
    11041 ccacgtcgcc cgaggcgcgc acgacgggct ggcccgtgcc atcacgcccg cgcacagccg
    11101 cgtcgacggc gacgccttcg tcgcggcggc caccggggcg gtcgatgcca ccgtcgagcg
    11161 ggtccgcgcc cacgccgtcg aggtggtcga ccgggcgatc cggcaggtcg cggagctggc
    11221 cgacctggcg cccggcgacc ggctggtccc gtgacccctc gaccgtgacc cctcgaccgt
    11281 gacgccggct gccgcccgcg cgtcgggctc cccgggcgcg tcccgttagg ctcggatcgt
    11341 ggctcccgtc gcagatgact cacccgtccc ggccgaccgg gccgagcggg ccgcggcgtt
    11401 cgctgagctg cgggaccggg cgctggagtg tcggcgctgc cgcctggccg agggccgcac
    11461 ccaggtcgtc ttcgggtccg gcgaccccga cgcggacctc atgttcgtgg gcgaagggcc
    11521 cggcgccgag gaggaccgcc agggcctgcc gttcgtcggc cggtccggtc agctgctcga
    11581 tcggctgatg gccgaggaga tggacctgac ccgcgatcgc gcctacatcg cgaacgtcgt
    11641 caagtgccga ccacccgaca accgggaccc caagctggac gagatcgagg cgtgccgccc
    11701 ctggctcgag gcccaggtcg acctgatcgg acccaaggtg atcgtcacgc tcgggaactt
    11761 ctcgtcgcgg ctgctgctcg acacccgcga ggggatcacc aggctccgag ggtcggtgta
    11821 cccgtaccgg gaccgccccg acggcacgcc cgtgcagctg gtgccgacgt tccaccccgc
    11881 cgcggtgctg cgcggcggcg gcgaaccgat ggccgggatg cgcgccgacc tggtgcgggc
    11941 caagcagacg ctcggcaccg ctgcgtcggc cgggaccggc gcgtcggtcg acgcgggagg
    12001 ctccgcgtga tcgatcaggc ccggctggtg gcgcggaccg cgtcggtgct cgagacacgt
    12061 gatctcgcgg cggcgttggc tgagctggcc cgcccgtcgg acctgctggt cctggtcggc
    12121 gacctcggcg cgggcaagac ggcgttcacc cagggcttcg gccgggggct cggcatcgac
    12181 gagcagatca ccagccccac cttcgctctc gtgcgcagct acaccggtcg tctcgacctg
    12241 taccacctgg acgtgtaccg gctcgagcag gtgtccgagg cgctcgacct ggggctgtcg
    12301 gagctgctcg acgacgggtc ggtgaccgtg atcgagtggg gcgacaccat cgccccggcc
    12361 ctgccgcacg actacctcga ggtccacctg cgcttcgacg tgcccgacgg cccggcgccg
    12421 acgggtgaga gctcggcccc tgcaaccgag gccgctgccg gggctgccgc cggctcggcg
    12481 gaggtgttcg agcgtcgcat cgtggagctg cgtccgatcg gtccgcggtg gtcggcgcgg
    12541 gtccgcgccc tgggcgcggc gctggcgccg tggaccgagg gcggcgccgg caacgacgcc
    12601 gacaccggca ccggcaacga cgccgacacc ggtgagcggg gcgcaccgac ggaacgggac
    12661 ggagtgggcg agtgctgatc ctgggcatcg agaccgcgac ggtgaaggcg ggctgcgcca
    12721 tcggaggcca cgagggcgtg ctcgcctcgg cgcactccgg caaggccaag cgccacgccg
    12781 agaacctcac cccggccatc gagttcatct gcggtcaggc cggcatcgag ctcggtgaga
    12841 tcggactggt cgccgtcgac gtcgggcccg gcctgttcac cggcctgcgc gtcggggtgg
    12901 cgacggccaa ggcgatcgcg ttcggcctgc gggtcccgat gatcggcgtg tcgagcctgg
    12961 acctgctggc gttcccggtc cggttcagca accgactgat cgtcaccgcc atcgacgccc
    13021 gccgaggcga gctctactac gccttctacc gacaggtccc cggcggcgtg caacgcctgg
    13081 gcgagcacca gatcggttca cccgacgacc tggccagcca gctgctcgcc agccccgacg
    13141 agatcctgct cgtcggcgac ggcgccaacc gctacgccga ggcgttcgag gggctggcca
    13201 aggtcgagct ggtcactcac gaccactgcc acccgtcggc cgagtcgctg gttcagctcg
    13261 cgcatgcccg ggcgctgcgc gaggagttcg agcggcccga cgcgatcgaa ccgatctacc
    13321 tgcggcgacc cgacgccgag atcaactggt cggtgcgcga tgcccgctga gcgctccgtg
    13381 ctcccgacgc accgtggccc gtcggtcgtc gctcccgctg gcgacggcgt ggtcgtggtc
    13441 ccgatgcggc gacgtcacct gcgcggcgtc gtcgacatcg agaaccgaac caaccatcgg
    13501 ccgtggtcgc tgtcgctgtt ccagggcgag ctgcggatgc cgacctcgcg ccgctatgtc
    13561 gtggcgctcg acgggcaccg cgtcgtcggc ttcgccgggc tgatgctcac cggtttcgag
    13621 ggccacatca ccaacgtggc cgtcgacccc gaccaccgcc gccggcacat cgcgacccgg
    13681 atgctgctcg tgttgttccg cgaggcgctg gcgctcgatg tcgaggacct gaccctcgag
    13741 gtgcgggtga ccaaccgtgg cgcccaggac ctgtaccggg gcttcggctt ctcgcccggc
    13801 ggcgtccggc ccaactacta caacgacatc aaggaggacg ccctcatcat gtgggctcac
    13861 gggatcggga ccgacgaggg tcgggcgcgc ctcgacgcgc tcaccgacgc gctgccggtc
    13921 gccctggccg aactggtggt ggaccgtgcc ggctgacgcg gcgtcacccc gactgctcgg
    13981 cggggccgcg gcgggttccg acgcgctcgt gctcggcatc gagacgtcgt gcgacgagac
    14041 cgccgcggcg tgcgtgcggg gcggcaccga cgtgctgtcg tcggtggtga gctcgcaggt
    14101 cgacctgcac gccaagtacg gcggcgtggt gcccgagatc gcgagccggg cccacaacga
    14161 gctgatcatc ccggtcgtgg cgcgggccat ggtcgaggcc ggcgtcgacg gtcgccgcat
    14221 cgacgccgtc gccgcgacca ccgggccggg cctgatcggc gccctgctgg tcggcgtgtc
    14281 ggcggccaag gcgctcgcgc tcacctggga cgtgccgttc gtgtcggtca accacctcga
    14341 ggcgcacctg tacgccgcgc tgctcgagga cccgcacctg gagctgccga tggtggtgct
    14401 gctggtgtcg ggcggccaca cgatgctggt gctgatggag gaccacggcc gctaccggat
    14461 cctcggccag accctcgacg acgcggccgg cgaggcgttc gacaaggtcg cccgcttcct
    14521 cggcctcggc taccccggcg gcccggcgat cgaccgtgag gccatggacg gcgaccccga
    14581 ggcggtgcgg ttccctcgct cgatgatcga cgacggcctc gacttctcct tcagcggcct
    14641 gaagaccgcg gtggtcaacc acgtgcgggc caaacccgat gtgtccaccc cggacgtggc
    14701 cgccagcttc caggccgcgg tggtcgacgt gctcgtcgcc aaggcgcggc gggcggcggc
    14761 ggagaccggg gcccgggcgt tggcgatcgg cggcggggtg gccgccaact cgctgttgcg
    14821 cgagcagttc ctgaccgcgt gcaccgacga cggcctgcac gccttcctgc ccagccgggc
    14881 catgtgcacc gacaacgcgg cgatgatcgc ctcggccggc tggtaccggt tgcgtgacga
    14941 cggaccgtcg ccgctgagca ccggagcgga ccccaacctg cggctgacca gccaccgctg
    15001 agggtcggca gcacgtgggg gaggcgtcca gcggagccgg cgagggcgcc gtcgccggcg
    15061 agcgggccgg aacaaacgag tgggccggag ctgacgaacc tgccggaccc gacgagtggt
    15121 ccggtcgctt cgagcagcag gccgcggcgt gcgacgatct cggctccccg ctgtgggccc
    15181 gcctcctgcg gatcgtcgcc gcggacgtcc gggccgaggg tccgtcgtgg tcggttctgc
    15241 gcgagcgggc cgggttgcgc ttcgggcagg ccggaccgct gcggttggtc ggcgccgcgc
    15301 accggctcgc gttggcgggg tcggcgccgg agtgggcgtc gctgctaccg agctgtggcg
    15361 gtgaggcccc gccggccgac gcggacggcg aacgggtcct ccgatccggg tgggcggggc
    15421 tggtcgacca ccacggtgac gcgctggtgg ccgggctgga ccgagaggtg cagaccaacg
    15481 aggtcggccg ggccgccgcg ctgggctacg cgcacgccgt cgccctcgcc gatcggggcg
    15541 ggccggcgag gttggtcgag atcggctgct ccggcgggtt gaacctgcgc ctggaccgct
    15601 tcggtctcga cttgtccggt ggcgggtccg gcccggtgct cggcgatccg gacggccggg
    15661 tgcggctggc gccggagatg tcgaccgtgg ctcgggccgg gctgtcgacg gtgggcgtcg
    15721 tcgagaggac cggcatcgat ccccacccga tcgacccgac gtcggtcgag gggcggctca
    15781 cgctgctgtc gttcgtgtgg cccgaccagg tcgagcgctt cgcgcggctc gccgccgcca
    15841 tcgacctggc ccgtgaggtc cccgccgagc tggtggcgac ggccgggcga cccaccgccg
    15901 aggtgctggc cgaggtgttg gcccgaccgg tcggggcggg cacggcgacg atcgtgcagc
    15961 actcgatcgt gtggcagtac atcccgaccg acgagcgggc cgcggtcacc tctgcgatcg
    16021 agtcggcggg ggagcgggcc acggccggtg agccggtcgt gtgggtgcgc ttcgaacccg
    16081 acgtgtggga ccgcgggcgg gtggcggtgt gggtcaggcg gtggcccgag gggggtgacc
    16141 ggctggtcgc ccacgccgac tacgccgacg cggcctgggt ggtgcccggg gtggcg
//
LOCUS       DAOQOD010000059        16091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1284_length_16091_cov_11.827014, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000059 DAOQOD010000000
VERSION     DAOQOD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_16091_cov_11.827014"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..859
                     /locus_tag="PLZ72_07595"
     CDS             122..859
                     /locus_tag="PLZ72_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNA36193.1"
                     /translation="MTGRRERAVVGMRLGAALAAELAGLALTVTVLSSPGSQQSTTTD
                     PLDHAGHVAGWVTIALLGYLLVVTGIQALALGLHGIAPGRPVAGRLAATATRLGPRAI
                     AGVAAAVLLSGATACQPTDPTPAGRDGQPVPVALEAVTPTVDATVSMVLESEPSEPVT
                     MELEAEPAVPTDPAAPAGEARTVVVGRGDNLWEIARREVSNRLGRPATATETDPYWRM
                     LVEINRPRLVDPTVPDLIYPGQVLQLP"
     gene            995..1987
                     /locus_tag="PLZ72_07600"
     CDS             995..1987
                     /locus_tag="PLZ72_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNA36194.1"
                     /translation="MQTVGLIVFIAVAVGFDFTNGFHDAANAVAASITTRAIQPMMAL
                     GMAAALNVVGALVSTKVAATVGEGIITIEPGTHGLVIVFSALLGAILWNLITWWFGLP
                     SSSTHALIGGLVGAGLASASVIQWESLISHVVIPMALSPVIGFGLGYLLMIAIIWMFR
                     HGRPHTVNHGFRRASVLSTAASAFSHGTQDAQKTMGIIALALVATGHLSSFSVPTWVI
                     LLAAAAMGLGTLAGGWRVIRTLGSRITPLDPPRAFAAQSSASAVLLVSAYVYALPVSS
                     THIMTTSIMGVGASRRLSAVRWSVAQRVAMAWILTIPGAAISAWVAYLVVNAVV"
     gene            complement(2019..3362)
                     /locus_tag="PLZ72_07605"
     CDS             complement(2019..3362)
                     /locus_tag="PLZ72_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNA36195.1"
                     /translation="MAARGDQKEFRRRVDDAVAVLFALVTEAISGGTSALLDHDTERA
                     QAIIDGDKGIDDRCDELIGVVKERLSSAVLDAEELEYLVAVLQIVPELERSADLAEHV
                     ASQTLENLSEVITARSRGLIQSMSDVAVQMWQSAGTAFRRGSRDAAPALREADDEIDD
                     MASRLVIEATREGADPQVAAQLALLARFYERLGDHAVNLAARVDQMAAPRRLVRLSRP
                     ERERRDSESLPEKRGGVRRVMHRLSRLRVVPHDDAFFVLFRSAAENAIECAGALGRLA
                     AANGDAAGALETVTECERRGDELTVELLRRLDASFVTPYDREDIHALTEEIDDVVDDM
                     FEAASVLQFALHDQRPPELLEIAELVVTMCDEMLALIECLETKNGARLRLERIEGLER
                     QADAAYRRGLARLFDGDYEPLEVIKLKDVLQSLEGSCNAVEDVSDVVESILVKNS"
     gene            3398..4201
                     /locus_tag="PLZ72_07610"
     CDS             3398..4201
                     /locus_tag="PLZ72_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36196.1"
                     /translation="MLTLVAVGLVVAAAAAFRGTWSPCGLSMLSTVTPLAESGRGHRY
                     RWTATWFFLGAVLGGATTGSVAAVAAVGIGALDLSAPTTYAIAGVAAVCAAGLDGRLL
                     GPPIPHHRRQVNEDWLGRYRAWVYGAGFGWQIGTGVATYIMSAGVYLVIALAALTGDP
                     RSALVVCVAFGAVRGSMVLIGARITSAERLARVHERFEAWREPVRRGVLGVLVLAAVV
                     LAVAGGAVAVVAAIASATVVSTIGAIRRVRRGSPAPAADACSIVGAAAT"
     gene            complement(4232..5518)
                     /locus_tag="PLZ72_07615"
     CDS             complement(4232..5518)
                     /locus_tag="PLZ72_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNA36197.1"
                     /translation="MEPIDPELLNRALAALGERFSMGVILFDGRLDLIWASGATSGVV
                     GWDVSEILGRNVLDLVHPDDIERVMPMLEVALDDPLARLNRSSSVRTAELSVRVTDPG
                     GGWRTVLVAGRVIDLDGNLVVTVRPAEERVAFDRVLQLLGRGERLSMTLDGVLDIVRA
                     HFEQPACIVHSVDGSTVVEGDREALVLDDADELLAALRDRGAPCESIDAIRWVFTVPS
                     AGRNDVVAAMVLKAPWADDPAPYDLAIIERVVGLAALAFERALFDRQLNQAATTDHLT
                     GLLNRRSFERELRSMPVDDGQPVLVLFADLDGLKEINDRHGHAVGDAVLAAVAARLTS
                     AVRSVDVVGRLGGDEFVVACPGLGDARRDDLIARVRSAVEGRVVVDGRDVPVRLSMGV
                     ATTADGAELATVIDRSDLEMYRDKRARRHLALTPPV"
     gene            complement(5602..6060)
                     /locus_tag="PLZ72_07620"
     CDS             complement(5602..6060)
                     /locus_tag="PLZ72_07620"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNA36198.1"
                     /translation="MSRTRKIGILMVLAALPALFACEAPPPAVAVGKIDISIKLQRAF
                     VYDTNGALMREIPVSSGAGGRTPLGNFRVYSRSSWTTSASDSRVSMKWMTRFNGGIGF
                     HGIPRRGSTPLATPLGVRPVSHGCVRMADADAEWIFHNVPNGTPVNVIPK"
     gene            6307..7419
                     /locus_tag="PLZ72_07625"
     CDS             6307..7419
                     /locus_tag="PLZ72_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA36199.1"
                     /translation="MRVPAAAISFPDEDREWILSQIDESLRSGQLTLGQIGRQLEAEF
                     AAAHDVAHAVSVSSGTSAVEIPLRVLECKGKEVLVQANTFFATAAAAVSAGADIRFVD
                     CDPATMAMDPAALQAAIGPNTVGVVVVHIGGYVAPAIRDIQRICADNGLWLFEDAAHA
                     HGSSLDGQFAGTFGVAGSFSFYPTKVIAGGEGGMLITNDSHFVDEAKIYRDQGKASFL
                     TNDHTRLGYNWRMSEPHAAIALSQLRRLDEFVDHRRHVASLYDAALADGPVTPLAIPA
                     ESRCNYYKYIAFLPPDVDRSFIKKRMREEFEVALSGEVYELPLHLQSVFEPWADGPLP
                     GAEELCARHICLPVSAVMTDEQVELVVRALNEVVAP"
     gene            7416..8348
                     /locus_tag="PLZ72_07630"
     CDS             7416..8348
                     /locus_tag="PLZ72_07630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA36200.1"
                     /translation="MTAASGTVAVTGGSGFIGSNVVDALIDSGRTVRVIDTVEPHRPD
                     VEWLPDADILDVESLLPAVEGTEAVFHLAAMADVNDIYADPARAVALNVQGTVNVLEA
                     GRRTGAGRTVLASTVWVYGSTVGEVVDETTPFTEDLDRHLYITTKISSEFACRDYLNL
                     YDRPFTVLRYGIPYGPRMRETTVMSAFFRRALSGEAITIDGDGMQSRNFVFVEDLAQA
                     HVLALRAEAENQVINVDGPVPVTIRQVAELTAQLAGDVEVSFGPSRPGDLKPQTVRND
                     KALDVLGWKPEVGIEDGMARSFEWYRANRAQGAS"
     gene            8350..9195
                     /locus_tag="PLZ72_07635"
     CDS             8350..9195
                     /locus_tag="PLZ72_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA36201.1"
                     /translation="MTSPRRIAVVPAYNEEPTVRDVLGKLYGYVDELVVVDDGSTDDT
                     RGEIEKFLAGRDGARMLVHDVNQGMSEAYYLAFGDLRRRLAAGELSPDDFVYTVDADG
                     QHELAVLEDLHRRMHDERLDALLVQRDLSTYPPYKQVGNWVMSTWATIWSGGVALKDV
                     ESGYRIFRLGALADALDYYQGYKYSETVEVAIVLSRLGYRVRNDVLVPVPVFRSRTRM
                     KDVVIDLAAMPAAAFRVTLRRPKDQPVPSLVPPVLTSAAIAAAAVAPPLAAVGLLRRR
                     RRSRR"
     gene            complement(9184..9690)
                     /locus_tag="PLZ72_07640"
     CDS             complement(9184..9690)
                     /locus_tag="PLZ72_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA36202.1"
                     /translation="MTPIERLIAHDQIRLLASRYAVAVDSRDLDALVRLFVDDVQVGR
                     DLRGRDALRESFRESLSAVGVTILNVGTHQIDLDAADAATGLVYCHGQVADGDRWIHQ
                     SILYRDTYARRNGEWLFVRRVHELWYGQAVENNPLEQPPAHWPTNPDGRGTVPESFET
                     WVGFWSAT"
     gene            complement(9693..10496)
                     /locus_tag="PLZ72_07645"
     CDS             complement(9693..10496)
                     /locus_tag="PLZ72_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36203.1"
                     /translation="MHTAGGRLTGRVALVTGAGEGIGRASARRLARDGAAVVIAELDG
                     ATGSDAERELRSDLGARAAFIATDVTVRDDVEAAVDRAVDLFGSIDVLVNNAWGGGTM
                     SRLEHKSDAQMAHGLQMAFWSAQWSMQRAFPHMRDAGGGSIINVCSLNGVNAHPFTAE
                     YNVGKEALRALTRTAAREWARHRIRANALCPGAATASFRAVEAAMPEMVAEILTQVPM
                     GYMGDPDDDIAGVVAFLASDDSRYLTGNTLFVDGGGHINGVPWAPDLPD"
     gene            complement(10520..11764)
                     /locus_tag="PLZ72_07650"
     CDS             complement(10520..11764)
                     /locus_tag="PLZ72_07650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA36204.1"
                     /translation="MTEQGSRRTALATGYGLATVALPIAALPAGSSTRDVVTVVVAVV
                     AAAAFAAVFVGWAATGRNGWVGAALVAVASLLYSVTPAGASTAIIALLVGAGTGLVAG
                     PRPWPDPRALRVAGTIAGVAVLGTAWALRGYGSSAVFLAAALGAAAVVLIRGLGERSD
                     LPESADRPEPTRRGRVLVGVAVAGAVGLVAWTGGNDPQLSWFGPVVSHGPRDRPEVAI
                     TFDDGPNESATLEVTRILDDHGVKGTFFLVGRAVAARPAVARQVVADGHLVGNHSYHH
                     DYWRWLDPRYPELGATQAVIGRDVGVCPRFFRPPHGQRTPLMNLQLARRGMVAVTWDV
                     SAADWVTNDPDLVARRILARVRPGSIILLHDSIDGHVDADRRVVIEALPRILDGLERK
                     GLQPVRLDRLIGGPAYLPDSDC"
     gene            11785..13125
                     /locus_tag="PLZ72_07655"
     CDS             11785..13125
                     /locus_tag="PLZ72_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36205.1"
                     /translation="MTDPSPPHPATLTTPDRQTPMLLRTGTVATAVIAAAWAVIGALM
                     LLRPVFISHDSLSNNVHVWFIADQLWSGHGIPMHVPALANGQALTFPYASIPWTTAAL
                     LWPLLGDRAVTLWLVLGFVATVVATFWTFPTLRRGWWAAATLTNPALVISPLLGQLPF
                     LWSIAAFTAAIGLWQRRRVVPAIALTAVASIIHPAVVMPIVALAVAAWLPFEDGDRRR
                     SLVGSWALTVLISLPAVWAVFQSPVVAQTSTATQVGALLQTVGMRLLVLAVPVALVLL
                     QRIPRAPRWTPVALTVVFVVLQVPMYTPFGMDFAWGSLTRDPDAQVDAFVASGAVRSG
                     DTYRVLTGRDGKYGLYAVARAGGVLDSELFPEGMHRGPFASTRSYAAFLRGRDVDTVV
                     WFPSYDARYTRSNESVLLERMAAEGCTDGVAVTRRDGAGPWRAYDVERGCRAQP"
     gene            complement(13116..13640)
                     /locus_tag="PLZ72_07660"
     CDS             complement(13116..13640)
                     /locus_tag="PLZ72_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNA36206.1"
                     /translation="MHSDAGGASHDDIALDDGGYARVVGERLREIRKQKKLSLQEVEK
                     VSELEFKASVLGAYERGERAISVPRLHRLARFYKVPVDQLLPGGDDPEDPARAEVDAS
                     GARIDLTRLEDIAGAEAEMLSRYLRMIQVQRQDFNGRVLTIRRDDMRAIACILGVTQE
                     SADQRLDELGLRQG"
     gene            complement(13805..14497)
                     /locus_tag="PLZ72_07665"
     CDS             complement(13805..14497)
                     /locus_tag="PLZ72_07665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNA36207.1"
                     /translation="MTRPPDASTQILVDDLGDPAGPSLGDDAFHHLSRALRLRPGESV
                     RAVDGAGGWVHCTFDGSTRLVPTGAAGLEAAPEQPISVAFVPVKGDRPELVVQKLTEL
                     GVDRIVITSSERSVVRWDGERAERHRRRLEKVSVEALQQCRRLWLPRIELIPLASLLA
                     DGAVLADMDGDRSVEVGRTVVVGPEGGWTDRERAEASRPAVALGPHVLRAETAAIAAG
                     VLLAAARERGGV"
     gene            complement(14516..15640)
                     /locus_tag="PLZ72_07670"
     CDS             complement(14516..15640)
                     /locus_tag="PLZ72_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNA36208.1"
                     /translation="MADLYEILEVSHDASMDEIKRNYRRLARECHPDANPDDPGAEAR
                     FKELAAAYEVLSDPDKRSRYDRFGSADGFDVGDPFGSGVSGLGDLFDAVFGGAGSPFG
                     GVRRTAGGPRRGPDLEAVATIEFNDAVFGTQTDVTVRTATACEDCGGSGAEAGSSPET
                     CTECGGSGEVRMVRQSVLGQIVTAAACRRCGGLGQTIASPCPTCGGDGCWVGDKTYTV
                     DVPRGVDHGSTLRLTGRGAVGPRGGPPGDLYVHLQVTPHERFVREGDDLVERIPIGFA
                     QAALGVKLPLETLDGWEDLVVPRGTQSGASFRLKGRGVPHLSGRGRGDLLVVVDVETP
                     TKLSDEEAELLRQYALLRGEDLSAPGEGLLSKVKSVFQGG"
     gene            complement(15644..>16091)
                     /locus_tag="PLZ72_07675"
     CDS             complement(15644..>16091)
                     /locus_tag="PLZ72_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36209.1"
                     /translation="TDLGAGTAPPGVAGAAIADAAVDAVRAAAADAGQQVYVDGAAHV
                     AGSFETRETVEQVLTILEQHFVVVSLLREMIDGGLTVAIGSETGVETLADCALVVAPY
                     SAGGTPAGAIAVLGPTRMDYAETVSTVSVVSRRLSRMLTDGRTAEG"
BASE COUNT         2206 a         6011 c         5578 g         2296 t
ORIGIN      
        1 cggcgacgat cgcggccccc gccgacggct gcggtgcacc ccgcgtcacc atgacggccg
       61 gcgcggtcca gaccgtcacg ccgaccgggt catcggacgc gacgcccgcc ggggaacgac
      121 cgtgaccggg cgacgggaac gcgccgtcgt cgggatgcga ctcggtgccg ccctggccgc
      181 cgagctcgcc ggtctggccc tcaccgtcac cgttctgagc tcccccggca gccagcagtc
      241 gacgacgacc gatccgctcg atcatgcggg gcacgtcgcg gggtgggtca cgatcgcgtt
      301 gctgggctac ctgctggtgg tcaccggcat ccaggcactg gccctggggc tgcacggaat
      361 cgcacccgga cgtccggtcg ccggacgcct cgcagcgacc gccacccgac tcgggccccg
      421 ggcgatcgcc ggtgtcgccg cggcggtcct gctctcgggt gcaaccgcct gtcagcccac
      481 cgatcccacc cccgcaggtc gcgatggcca gcccgttccg gtggcactgg aggcggtgac
      541 ccccacggtc gacgcgacgg tctcgatggt tctggaatcg gagccgtcgg agccggtcac
      601 catggaactc gaggcggagc ccgctgtccc gaccgatccg gctgcgccgg ccggtgaggc
      661 ccggacggtc gtcgtcggcc gaggagacaa cctctgggag atcgcccgac gcgaggtgtc
      721 gaaccggctc ggccgccctg ccaccgcgac cgagaccgac ccgtactggc ggatgttggt
      781 cgagatcaac cgcccccgac tggtcgaccc gaccgtgccc gacctcatct accccggcca
      841 ggtgctccaa ctaccctgac caccggtcag cctcgctcgg tacgtaccgt cccgcgccgg
      901 gatcggccgg atctgcccgt tccgcgcagg cgctactctc cgacccgatg cgcgccggcc
      961 ccccgacccg atcccgtcgg agcgggcccc gctgatgcag accgtcggcc tgatcgtgtt
     1021 catcgcggtg gcggtgggct tcgacttcac caacgggttc cacgacgccg ccaacgccgt
     1081 cgccgcgtcg atcaccaccc gggcgatcca gccgatgatg gccctgggca tggccgcggc
     1141 gctcaacgtg gtcggcgccc tggtgtccac aaaggtcgcg gccaccgtcg gcgagggcat
     1201 catcacgatc gagcccggca cccacggtct ggtcatcgtg ttctcggcgc tgctcggagc
     1261 gatcctctgg aacctgatca cgtggtggtt cgggctgccg tcctcgtcga cccacgccct
     1321 catcgggggc ctggtgggag ccgggttggc ctcggcgagc gtcatccagt gggagtcgtt
     1381 gatcagccac gtcgtcatcc cgatggcgct gtccccggtg atcggcttcg gcctcggcta
     1441 cctgctgatg atcgccatca tctggatgtt ccgccatggt cgaccgcaca ccgtgaacca
     1501 cggcttccgg cgggcgtcgg tgctgtccac cgccgccagc gcgttcagcc acgggaccca
     1561 ggacgcccag aagacgatgg gcatcatcgc actcgccctg gtcgcgacag gtcacctgtc
     1621 gagcttcagc gtcccgacgt gggtgatcct gctggccgcc gcggcgatgg gcctgggcac
     1681 gctcgccggg gggtggcggg tcatccgcac cctcggcagc cgcatcacgc cgctcgatcc
     1741 gccccgggcg ttcgccgctc agagctcggc atccgctgtg ctgctggtgt cggcctacgt
     1801 gtatgccctc ccggtgtcct cgacgcacat catgaccacg tcgatcatgg gagtcggggc
     1861 cagccgacgt ctctcggcgg tgcgctggag cgtcgcccaa cgggtcgcca tggcctggat
     1921 cctcaccatc ccgggtgccg cgatctcggc gtgggtcgcc tacctggtgg tcaacgccgt
     1981 cgtctgagcg ggatcccgcg aatcgacgac cgggccggtc aggagttctt gaccaggatc
     2041 gactcgacca cgtcggagac gtcctcgacc gcgttgcagg agccctccag ggactgaagc
     2101 acgtccttga gcttgatcac ctcgagaggt tcgtagtcgc cgtcgaagag ccgggccagg
     2161 ccgcgccggt aggcggcgtc ggcctgacgc tccaggccct cgatgcgctc caggcggagc
     2221 cgggcgccgt tcttggtctc gaggcactcg atgagggcga gcatctcgtc gcacatggtc
     2281 acgaccagct cggcgatctc cagcagctcg ggcgggcgct ggtcgtggag cgcgaactgg
     2341 agcaccgacg cggcctcgaa catgtcgtcg acgacgtcgt cgatctcctc ggtgagggcg
     2401 tggatgtcct cgcggtcgta cggcgtgacg aagctcgcgt cgagtcgtct cagcagctcc
     2461 acggtgagct cgtcgccgcg ccgttcgcac tcggtgaccg tctcgagcgc accggcggcg
     2521 tcgccgttcg ctgcggccag gcggccgagc gccccggcgc actcgatcgc gttctcggcg
     2581 gcggaacgga acagcacgaa gaaggcgtcg tcgtggggca ccacccggag tcggctgagc
     2641 cggtgcatga cccgacgcac cccgccgcgc ttctcgggca gcgactcgga gtcgcgccgc
     2701 tcccgctccg gccgcgacag gcggaccagc cgtcgtgggg cggccatctg gtcgacccgg
     2761 gcggccaggt tcaccgcgtg gtcaccgagc cgctcgtaga agcgggcgag gagggcgagc
     2821 tgcgccgcga cctgcggatc cgcgccctcg cgggtggcct cgatcaccag ccggctcgcc
     2881 atgtcgtcga tctcgtcgtc ggcttcgcgc agcgccgggg cggcgtcgcg cgatccccgt
     2941 cggaacgcgg tcccggccga ctgccacatc tgcaccgcca cgtcgctcat cgactggatc
     3001 aacccgcggc tgcgggcggt gatgacctcg ctcaggttct cgagagtctg actggcgacg
     3061 tgctcggcga gatcagcgct tcgttcgagc tcgggcacga tctgcaggac cgcgaccagg
     3121 tactcgagct cctcggcgtc gagcacggcg ctcgacagcc gctccttgac cactccgatg
     3181 agctcgtcgc agcggtcgtc gatgcccttg tcgccgtcga tgatcgcctg ggcccgctcg
     3241 gtgtcgtgat cgaggagggc cgaggtgccg ccgctgatcg cctcggtcac cagggcgaac
     3301 agcaccgcga ccgcgtcgtc gacccggcgg cggaactcct tctggtcacc tcgtgcagcc
     3361 accgcagcag ggtatctgct ccgcgtagga tggccggatg ctgacactcg tcgcggtggg
     3421 cctcgtcgtt gccgcagcag ccgcgttccg ggggacctgg tcgccgtgcg gcctgtcgat
     3481 gctgtcgacg gtgaccccgt tggccgagtc cggtcggggc caccggtacc ggtggaccgc
     3541 gacgtggttc ttcctgggcg cggtgctggg cggcgccacc accggctcgg tcgccgcggt
     3601 cgccgcggtc ggcatcgggg cgctcgacct gtccgccccg accacctacg cgatcgccgg
     3661 agtcgccgcg gtgtgcgccg ccgggctcga cggacggctt ctcggcccgc cgatccccca
     3721 ccaccgccgc caggtcaacg aggactggct cggccgctac cgggcatggg tctacggggc
     3781 cgggttcggc tggcagatcg gcaccggcgt cgccacctac atcatgagcg ccggcgtgta
     3841 cctggtcatc gccctcgccg cgctgacggg cgaccctcgc agcgccctgg tggtgtgcgt
     3901 cgccttcggc gccgtccgcg gctcgatggt gctgatcgga gcacggatca cctccgccga
     3961 acgtctggcc cgggtgcacg aacgcttcga ggcgtggcgc gagccggtcc ggcgcggcgt
     4021 cctgggcgtg ctcgttctgg ccgccgtcgt cctcgccgtc gccggtgggg ccgtcgccgt
     4081 cgtcgcagcc atcgcgtcgg ccaccgtcgt gtcgacgatc ggcgccatcc gccgcgtccg
     4141 ccgcgggtcg ccggccccgg ccgcggatgc ctgctcgatc gtgggggccg ccgccacctg
     4201 acacggcgac cgcgtcagac gtgagggcgc ttcagaccgg aggcgtcagg gcgaggtgcc
     4261 gccgtgcccg cttgtcgcgg tacatctcca ggtcgctgcg gtcgatcacc gtcgccagct
     4321 ccgcgccgtc ggccgttgtg gcgacgccca tcgacaaccg gacgggcacg tcacggccgt
     4381 cgacgacgac ccgcccctcg accgcggagc ggacccgagc gatcagatcg tcacgcctgg
     4441 cgtcgccgag cccgggacac gcgacgacga actcgtcgcc gccgaggcgg cccacgacgt
     4501 cgacggagcg caccgcggag gtcaaccggg cggccaccgc ggcgagcacc gcatcgccga
     4561 cggcgtggcc gtgtcgatcg ttgatctcct tcagcccgtc gaggtcggcg aacaggacca
     4621 ggaccggctg tccgtcgtcg accggcatcg agcgcagctc ccgttcgaag ctgcgccggt
     4681 tcaggagacc ggtcaggtgg tcggtggtcg cggcctggtt gagctgccgg tcgaagagcg
     4741 cccgttcgaa ggcgagggcg gccaggccga cgaccctctc gatgatcgcg aggtcgtacg
     4801 gcgcggggtc atcggcccac ggggccttga gcaccatcgc ggcgacgacg tcgttgcggc
     4861 ccgccgacgg cacggtgaac acccatcgga tcgcgtcgat cgactcgcac ggcgcgccac
     4921 ggtcgcgcaa cgcggcgagg agttcgtccg cgtcgtcgag caccagcgcc tcccgatccc
     4981 cctccaccac ggtggacccg tccacggagt ggacgatgca cgccggctgc tcgaagtgcg
     5041 cccgcacgat gtcgagcacc ccatcgaggg tcatggagag gcgctcgccg cggccgagga
     5101 gttgcagcac ccggtcgaac gcgacacgtt cctcggccgg acggacggtg accaccaggt
     5161 tgccgtcgag atcgatcacc cggcccgcca cgaggaccgt gcgccatccc cctcccggat
     5221 cggtgacccg caccgacagc tcggcggtcc ggaccgagga cgagcggttg agccgtgcca
     5281 gcgggtcatc cagggcgacc tcgagcatgg gcatgacccg ctcgatgtcg tcggggtgga
     5341 cgaggtcgag cacgttgcgg cccaggatct cggagacatc ccacccgacg acgccggagg
     5401 tcgccccgct cgcccagatg aggtcgaggc gaccgtcgaa gaggatcacg cccatcgaga
     5461 agcgctcgcc gagggcggcc agcgcacggt tcagcagctc gggatcgatc ggctccacac
     5521 ggtcccatcg gccgcgaccc ccgccggatt gaacctcgag ggccgaagga cccacgggcc
     5581 ggcgcccggg tgccgatcag ctcacttcgg gatgacgttg accggggtgc cgttgggcac
     5641 gttgtggaag atccactcgg catcggcgtc ggccatgcgc acgcagccgt gcgacaccgg
     5701 ccggacgccc agcggggtcg ccaggggggt cgagccccga cgggggatcc cgtggaaacc
     5761 gatgccgccg ttgaagcggg tcatccactt catcgacacc cgggaatccg acgcggacgt
     5821 cgtccacgac gaacggctgt agacccggaa gttgccgagc ggtgtgcggc ccccggcgcc
     5881 gctcgagacg gggatctcgc gcatgagcgc gccgttggtg tcgtagacga acgcccgctg
     5941 gagcttgatc gagatgtcga tcttgccgac cgccaccgcc ggcgggggcg cctcgcaggc
     6001 gaacagcgcc ggcagagccg ccagcaccat gaggatcccg atcttcctgg tcctgctcac
     6061 gacgccttcg ctcctcatcg ctgctccagc ggtccgccgc ggaccgtggg gtcggtcaag
     6121 tgtgccaagg cggccacacg gcgccaactc ctgggcccga cccggaccgg cggcgggggc
     6181 gccgatgacg gtgtcggagg acctcaacgg gtagtgtcgg cgacatctga cactggccgg
     6241 atcggccgaa ccgctgggtt cgctccggcc ggtcgacccc cctgcacaga aaggcacggc
     6301 cgccgaatgc gcgtccccgc agcagccatc tcgttccccg acgaggaccg ggagtggatc
     6361 ctcagccaga tcgacgagag cctccggtcc gggcagctga ccctcggcca gatcggccgt
     6421 cagctcgaag ccgagttcgc cgccgcccac gacgtggccc acgccgtgtc ggtcagctcg
     6481 ggaaccagcg cggtcgagat cccgctgcgg gtcctcgagt gcaagggcaa ggaggtgctg
     6541 gtccaggcca acaccttctt cgccaccgcg gcggctgcgg tgtccgcagg ggccgacatc
     6601 cgcttcgtcg actgcgaccc ggccaccatg gccatggacc ccgccgcgct gcaggccgcc
     6661 atcggcccca acaccgtcgg ggtggtcgtg gtccacatcg gcggctacgt cgccccggcc
     6721 atccgcgaca tccagcgcat ctgcgccgac aacggcctct ggctgttcga ggacgccgcg
     6781 cacgcccacg gcagctcgct cgacgggcag ttcgccggca ccttcggcgt cgccggatcg
     6841 ttcagcttct accccaccaa ggtcatcgcc ggcggtgagg gcggcatgct catcaccaac
     6901 gacagccact tcgtcgacga ggccaagatc taccgcgacc agggcaaggc gtcgttcctg
     6961 accaacgacc acacccgtct gggctacaac tggcggatga gcgagccgca cgccgcgatc
     7021 gcgctcagcc agctgcgccg cctcgacgag ttcgtcgatc accggcgcca cgtcgcctcg
     7081 ctgtacgacg ccgcgctggc cgacggtccg gtcaccccgc tcgcgatccc tgccgagtcg
     7141 cgctgcaact actacaagta catcgccttc ctgccgccgg acgtcgaccg ctccttcatc
     7201 aagaagcgca tgcgcgagga gttcgaggtc gccctctccg gtgaggtcta cgagctcccg
     7261 ctgcacctcc agtcggtctt cgagccgtgg gccgacggcc cgctgcccgg cgccgaggag
     7321 ctgtgcgccc gccacatctg cctgccggtc tccgcggtga tgaccgacga gcaggtcgaa
     7381 ctggtcgtcc gcgccctcaa cgaggtggtg gcaccgtgac cgccgcctcc ggcaccgtcg
     7441 cggtcaccgg cggatcgggc ttcatcggct ccaacgtcgt cgacgccctg atcgactcgg
     7501 gccgcaccgt ccgggtgatc gacaccgtcg agccgcaccg ccccgacgtc gagtggctcc
     7561 ccgacgccga catcctcgac gtcgagtcgc tgctgcccgc ggtcgagggc accgaggcgg
     7621 tgttccacct cgccgccatg gccgacgtca acgacatcta cgccgatccc gcccgggcgg
     7681 tcgcgctcaa cgtgcagggc acggtcaacg tgctcgaggc cggccgccgc accggtgccg
     7741 gtcgcacggt cctggcctcg acggtgtggg tgtacggctc gaccgtcggc gaggtcgtcg
     7801 acgagaccac cccgttcacc gaggacctcg accgccacct ctacatcacg accaagatct
     7861 cctcggagtt cgcgtgtcgg gactacctga acctctacga ccgtccgttc accgtgctgc
     7921 gctacgggat cccgtacggc ccccgcatgc gcgagacgac cgtgatgagc gccttcttcc
     7981 gccgtgcgct gtcgggcgag gcgatcacca tcgacggcga cggcatgcag agccgcaact
     8041 tcgtcttcgt cgaggacctg gcccaggccc acgtcctcgc cctgcgagcc gaggccgaga
     8101 accaggtgat caacgtcgac ggtccggtgc cggtcaccat ccgccaggtg gccgagctga
     8161 ccgcccagct ggccggcgac gtcgaggtct cgttcggccc gtcgcgcccc ggtgacctca
     8221 agccgcagac cgtgcgcaac gacaaggcgc tcgacgtcct cggctggaag cccgaggtcg
     8281 gcatcgagga cggcatggcc cgcagcttcg agtggtaccg cgccaaccgg gcgcagggag
     8341 cgtcctgagg tgaccagccc ccgccggatc gcggtcgtcc ccgcctacaa cgaggagccg
     8401 accgtccgcg acgtgctcgg caagctctac ggctacgtcg acgagctcgt cgtggtcgac
     8461 gacggctcca cagacgacac ccgcggcgag atcgagaagt tcctcgccgg ccgcgacggc
     8521 gcccggatgc tggtccacga cgtcaaccag ggcatgagcg aggcctacta cctggccttc
     8581 ggcgacctgc ggcgccgcct ggccgccggc gagctgtcgc ccgacgactt cgtgtacacc
     8641 gtcgacgcgg acggccagca cgagctcgcg gtgctcgagg acctgcaccg ccgcatgcac
     8701 gacgaacgcc tcgacgcgct gctggtgcag cgggacctgt cgacgtatcc gccctacaag
     8761 caggtcggca actgggtgat gagcacctgg gccaccatct ggtccggtgg cgtggcgctc
     8821 aaggacgtgg agtccggcta ccggatcttc cgcctcggcg cgctggccga cgcgctcgac
     8881 tactaccagg gctacaagta ctccgagacc gtcgaggtgg ccatcgtgct gtcgcggctc
     8941 ggctaccggg tccgcaacga cgtgctcgtg cccgtgccgg tgttccgctc ccggacccgc
     9001 atgaaggacg tggtcatcga cctggccgcc atgcccgccg ccgcgttccg ggtgaccctg
     9061 cgccgaccga aggaccagcc ggtccccagc ctggtgccgc cggtgctcac ctcggccgcc
     9121 atcgccgccg ccgctgtggc cccgccgctc gcggccgtcg ggctcctgcg gcgtcgccgt
     9181 cgctcacgtc gctgaccaga agccgaccca ggtctcgaag ctctcgggga ccgtgccccg
     9241 accgtccggg ttggtcggcc aatgggcggg cggctgctcc agcgggttgt tctccaccgc
     9301 ctggccgtac cagagctcgt ggacccgacg gacgaacagc cactcgccgt tccgacgggc
     9361 gtaggtgtcg cggtacagga tcgactggtg gatccaccgg tcgccgtcgg cgacctgccc
     9421 gtggcagtac accaggcccg tcgccgcgtc cgcggcgtcc agatcgatct ggtgcgtgcc
     9481 gacgttgagg atggtgaccc ccaccgcgga caacgactcc cggaacgact ctcgcagcgc
     9541 gtcacgacca cggaggtccc taccgacctg cacgtcgtcg acgaacagac gaaccagcgc
     9601 gtcgaggtcc cgggagtcga ccgccaccgc gtagcggctc gccagcagtc ggatctggtc
     9661 gtgggcgatg agtcgttcga tcggggtcac ggtcagtccg gcaggtcggg cgcccacggc
     9721 acgccgttga tgtggccacc gccgtcgacg aagagggtgt tgccggtcag gtagcgggag
     9781 tcgtcggagg ccaggaacgc caccaccccg gcgatgtcgt cgtcggggtc gcccatgtag
     9841 cccatcggga cctgggtgag gatctcggcc accatctcgg gcatggccgc ctcgaccgcc
     9901 cggaacgacg ccgtggccgc ccccgggcac agggcgttgg cccggatgcg atgacgggcc
     9961 cactcgcggg ccgcggtgcg ggtgagcgcc cgcaacgcct ccttgccgac gttgtactcg
    10021 gcggtgaacg gatgggcgtt gacgccgttg agcgagcaca cgttgatgat cgagccgcct
    10081 ccggcgtcgc gcatgtgggg gaaggcgcgc tgcatggacc actgcgccga ccagaacgcc
    10141 atctgcaggc cgtgggccat ctgcgcgtcg ctcttgtgct cgagccgcga catggttccg
    10201 ccgccccagg cgttgttgac caggacgtcg atcgagccga acaggtcgac ggcccggtcg
    10261 accgccgcct ccacgtcgtc gcggacggtg acgtcggtgg cgatgaacgc ggcacgggca
    10321 cccaggtcgg aacgcagctc gcgctcggcg tcggagcccg tcgcaccgtc gagctcggcg
    10381 atgaccaccg ctgccccgtc gcgagccaga cgccgcgccg aggcccgacc gatcccctcg
    10441 ccggccccgg tcaccaacgc gacccgacct gtcagacgcc ccccggcggt gtgcacggcc
    10501 ggcacgctag cgcccgcgat cagcagtcgg agtcgggcag gtatgccggt ccgccgatga
    10561 gccggtcgag gcggaccggt tggagaccct tgcgctccag cccgtcgagg atcctgggca
    10621 gggcttcgat caccacccgg cgatccgcgt cgacgtggcc gtcgatgctg tcgtgcagca
    10681 ggatgatcga ccccggccgc acccgggcca ggatccgccg ggccaccagg tcgggatcgt
    10741 tcgtgaccca gtccgcggcg gagacgtccc aggtgaccgc caccatcccc cgacgggcga
    10801 gctgcaggtt catcagcggg gtgcgctgcc cgtgcggggg ccggaagaag cgggggcaca
    10861 cgcccacgtc ccggccgatg acggcctggg tggcgccgag ctcgggatag cggggatcga
    10921 gccaccgcca gtagtcgtgg tggtacgagt ggttgccgac caggtgcccg tccgcgacga
    10981 cctgccgggc caccgcgggg cgggcggcga cggcccgccc gaccaggaag aacgttccct
    11041 tcacgccgtg gtcgtcgagg atccgggtca cctcgagggt ggccgactcg ttgggtccgt
    11101 cgtcgaaggt gatcgccacc tcgggccggt cgcgggggcc gtgggagacg acaggaccga
    11161 accagctcag ctgcgggtcg ttgccgccgg tccacgccac cagcccgacc gccccggcca
    11221 ccgcgacgcc gaccaggacc cggccccgac gggtcggctc gggacggtcg gcggactccg
    11281 gcaggtcgga gcgttccccg agtccgcgga tgagcaccac cgccgcggcg ccgagcgcgg
    11341 cggcgaggaa caccgccgag gagccgtacc cgcgcagcgc ccaggccgtg ccgagcaccg
    11401 ccaccccggc gatcgtgccg gccacgcgca gagcgcgggg atcgggccac ggtcggggcc
    11461 cggccacgag gccggttccg gcgccgacca gcagggcgat gatggcggtc gaggcacctg
    11521 ccggggtcac cgagtacagc agggacgcga ccgcgaccag cgccgctccg acccacccgt
    11581 tgcgcccggt ggcggcccac ccgacgaaca ccgccgcgaa cgccgctgct gcgaccacgg
    11641 cgacgacgac ggtgacgaca tcacgggtcg aggaccccgc cggcagcgcc gcgatcggca
    11701 gggcgacggt tgccagcccg tatcctgtgg ccagcgcggt ccggcgactc ccctgttcgg
    11761 tcatccgccc gcctccgatc gtccatgacc gacccgtctc cgccgcaccc ggccacccta
    11821 acgacccccg accggcagac cccgatgctg ctgaggaccg gcaccgtggc caccgcggtg
    11881 atcgcggcgg cctgggccgt gatcggggcc ctgatgctgc tgcggccggt gttcatcagc
    11941 cacgactcgc tgtcgaacaa cgtccacgtc tggttcatcg cggatcagct ctggagcggc
    12001 cacgggatcc cgatgcacgt cccggcgctc gccaacggcc aggccctcac cttcccgtac
    12061 gcgtcgatcc cgtggaccac cgccgccctg ctgtggccgc tgctcggcga ccgggctgtc
    12121 accctctggc tggtcctcgg cttcgtggcc accgtcgtcg ccacgttctg gaccttcccc
    12181 accctgcggc ggggctggtg ggccgcggcg acgctgacca acccggccct ggtcatctca
    12241 ccgctgctcg ggcagctccc gttcctctgg tcgatcgccg cgttcaccgc cgcgatcggg
    12301 ctctggcagc ggcgccgggt ggtcccggcg atcgccctga ccgctgtcgc gtcgatcatc
    12361 caccccgcgg tcgtgatgcc gatcgtcgcg ctcgcggtcg cggcgtggct gccgttcgag
    12421 gatggggatc ggcgccgatc gctcgtcggc tcctgggcgc tcacggtcct gatcagcctc
    12481 ccggcggtgt gggcggtgtt ccagtcgccg gtggtcgccc agacctcgac cgccacccag
    12541 gtgggcgctc tgctgcagac cgtgggaatg cgcctgctcg tcctggccgt gcccgtcgcg
    12601 ctcgtcctcc tgcagcggat cccccgggcc cctcgttgga cgccggtcgc tctcaccgtc
    12661 gtgttcgtgg tcctgcaggt gccgatgtac acgccgttcg gcatggactt cgcgtggggc
    12721 agcctcaccc gggaccccga cgcgcaggtc gacgccttcg tcgcgtccgg ggcggtccgc
    12781 tccggcgaca cctaccgggt gctcaccggc cgcgacggca agtacggcct gtacgcggtc
    12841 gcccgggccg gcggcgtgct cgactcggag ctcttccccg agggcatgca ccgggggccg
    12901 ttcgccagca cccgctccta cgcggcgttc ctgcgcggtc gcgacgtgga caccgtggtg
    12961 tggttcccga gctacgacgc ccgctacacg cgctccaacg agagcgtcct gctcgagcgc
    13021 atggcggccg agggctgcac cgatggcgtg gcggtcaccc gtcgggacgg tgccggcccg
    13081 tggcgggcct acgacgtcga gcgggggtgt cgggctcagc cctgacggag tccgagctcg
    13141 tcgaggcgct ggtcggccga ctcctgggtc acccccagga tgcaggcgat ggcccgcatg
    13201 tcgtcacggc ggatggtgag cacccgaccg ttgaagtcct ggcgctgcac ctggatcatc
    13261 cgcaggtagc ggctcagcat ctcggcctcg gcgccggcga tgtcctcgag gcgggtcagg
    13321 tcgatccggg cccccgaggc gtcgacctcg gcccgggcgg ggtcctcggg gtcgtcgccg
    13381 ccgggcagga gctggtcgac gggcaccttg tagaagcggg ccaaccggtg cagccggggg
    13441 accgagatgg cccgctcgcc ccgctcgtag gcgccgagca ccgacgcctt gaactcgagc
    13501 tcggagacct tctcgacctc ctgcagcgag agcttcttct gcttgcggat ctcacgcaac
    13561 cgctcaccga cgacccgggc gtagcccccg tcgtccaggg cgatgtcgtc gtggctcgct
    13621 ccacctgcgt ctgagtgcac cgtcgatcct tcgtccgtcg ggccgcccgc ccacccacgg
    13681 tcgggatcgg cacggtcggg ttcgaccacc actgtgagtc actctgcgaa cccgatccac
    13741 ggggaaaagt accctcaccg acacgctccg tggcaatcgg gcgccaccgg tggagcgaca
    13801 gccgtcagac gccgccacgc tcccgggcgg cagcgagcag cacgccggcg gcgatcgcgg
    13861 cggtctcggc ccgcaggacg tgaggcccca gcgcgaccgc cgggcggctc gcctcggcgc
    13921 gctcccggtc ggtccatccg ccctcggggc cgacaacgac ggtgcggccg acctcgaccg
    13981 accggtcgcc atccatgtcg gcgagcaccg ccccgtcggc cagcagggac gccagcggga
    14041 tcagctcgat ccggggcaac cagagtcgtc ggcactgctg gagcgcctcg acgctgacct
    14101 tctccagccg ccggcggtgc cgctcggccc gctccccgtc ccagcgcacc accgatcgct
    14161 ccgaggaggt gatgacgatc cggtcgacgc ccagctcggt gagcttctgg acgacgagct
    14221 ccggtcggtc gcccttcacc ggcacgaacg cgaccgagat cggctgttcg ggcgcggcct
    14281 cgagcccggc cgcgccggtg gggaccaggc gggtcgaacc gtcgaaggtg cagtggaccc
    14341 agccgccggc cccgtcgacg gcgcgcaccg actcccccgg gcgcagtcgc agcgcccggg
    14401 acaggtgatg gaacgcgtcg tcgccgaggg acgggcccgc cgggtcgccg aggtcgtcga
    14461 cgaggatctg ggtcgaggcg tccggcggtc gggtcacgct cggagcatgc tgccgtcagc
    14521 cgccctggaa gacggacttg accttggaga gcaggccctc acccggagcc gagaggtcct
    14581 cgccacgcag cagcgcgtac tggcggagca gctccgcctc ctcgtcgctc agcttggtcg
    14641 gcgtctcgac gtcgaccacg accagcaggt cgccccggcc gcgcccgctc aggtgcggga
    14701 cgccgcggcc cttcaggcgg aagctcgccc cgctctgggt gcccctgggc acgacgaggt
    14761 cctcccagcc gtcaagagtc tcgagcggca gcttgacgcc gagcgcggcc tgggcgaacc
    14821 cgatcgggat ccgctcgacc aggtcgtcgc cctcgcgcac gaagcgctcg tgcggggtca
    14881 cctggaggtg cacgtacagg tcgccggggg ggccgccgcg cgggccgacg gccccgcgtc
    14941 cggtcaggcg cagggtggag ccgtggtcga ccccgcgggg gacgtcgacg gtgtaggtct
    15001 tgtcgcccac ccagcagccg tcgccgccgc aggtcggaca cggcgacgcg atcgtctgac
    15061 ccagcccgcc gcagcgtcgg cacgcggccg cggtcacgat ctgacccagc acgctctggc
    15121 ggaccatccg cacctcgccg gagccgccgc actcggtgca ggtctcgggc gaggaaccgg
    15181 cctcggcccc gctccctccg cagtcctcgc acgcggtcgc ggtccggacc gtgacgtcgg
    15241 tctgcgtgcc gaacaccgcg tcgttgaact cgatcgtggc gaccgcctcg aggtccggcc
    15301 cccgtcgggg accgccggcg gtgcgccgga cacccccgaa cgggcttccc gccccgccga
    15361 acacggcgtc gaagaggtcg cccaggcccg acaccccgga gccgaacggg tcgccgacgt
    15421 cgaagccgtc ggcggagccg aaccggtcgt accggctgcg cttgtcgggg tccgacagca
    15481 cctcgtaggc cgccgcgagc tccttgaacc gggcttcggc gccggggtcg tcggggttcg
    15541 cgtcgggatg gcactcccgg gccaggcgcc ggtagttgcg cttgatctcg tccatcgagg
    15601 cgtcgtggga cacctcgagg atctcgtaca ggtcagccac ccgtcacccc tcggccgttc
    15661 gtccgtcggt cagcatccgg ctcagccggc ggctcacgac cgacacggtg gagaccgtct
    15721 cggcgtagtc catgcgcgtc ggccccagca ccgcgatcgc tccggcggga gtccctcccg
    15781 ccgagtacgg agccacgacc agcgcgcagt ccgcgagcgt ctccacaccg gtctccgagc
    15841 cgatggcgac ggtgagaccg ccgtcgatca tctcccgcag cagcgacacc acgacgaagt
    15901 gctgctcgag gatggtcagg acctgctcga cggtctcgcg cgtctcgaac gagccggcga
    15961 cgtgcgcggc gccgtcgacg tagacctgtt ggccggcatc ggccgccgca gcccgcaccg
    16021 cgtcgaccgc cgcgtccgcg atggccgcgc cggcgacgcc cggaggggcg gtcccggcgc
    16081 ccagatcggt c
//
LOCUS       DAOQOD010000060        16090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1285_length_16090_cov_9.739757, whole genome shotgun sequence.
ACCESSION   DAOQOD010000060 DAOQOD010000000
VERSION     DAOQOD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16090
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_16090_cov_9.739757"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLZ72_07680"
     CDS             <1..696
                     /locus_tag="PLZ72_07680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070677 - rRNA
                     (cytosine-2'-O-)-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNA36210.1"
                     /translation="HAGIPGKRLVRLDDHTEAAVTADLVARAAAGDRVALVSDAGLPG
                     LSDPGAVLVAAAWSAGVAVEVVPGPFAAAVGLVGSGMLEPSGRFCFEGFLPRKGPARR
                     TRIAALVGEERTTVLYEAPHRLRSTLEDLRDALGADRRVALCRELTKLHEETWRGSLG
                     DAVARCAEVEPRGEFVVVLAGAEPPPDATDDELRRSLDRELETGASRRDAAATVAARF
                     DVAPNRVKRLLSG"
     gene            complement(722..1939)
                     /gene="larC"
                     /locus_tag="PLZ72_07685"
     CDS             complement(722..1939)
                     /gene="larC"
                     /locus_tag="PLZ72_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNA36211.1"
                     /translation="MSGTGDRTPSGLWINPAVGVAGDMLLAALVDLGASLDAVRAAVG
                     SLGIDGWTLDAGGTTRRGLTATSVTVHAEDGQRHRPWSAIDARLADADLPAAISRGAR
                     RTFELLARAEAAVHGVEMDQVHFHEVGAIDALVDIVGTWAAWSDLGQPDVSAGPVGLG
                     VGTVAMAHGTVAVPAPATLELLRRIPTVPVDAAGETATPTGVALLVSMVDSPDRWGPM
                     PAGTVHSVGRGAGSWDPPGHANVVTAVAFEPTDPTADREVGVETVLIETVLIETTVDD
                     VTPEVLGHVIDAAIAMGADDAWVLPVTMKKSRPGHQVRVLCSPPLVPEVRGLLARETG
                     TLGWREWSVTKHELPRHETVVELDGRPVRIKVSPHGAKPEHDDVVGIARASGRSARDV
                     AADAAAAFRDTLP"
     gene            complement(1936..3483)
                     /gene="larB"
                     /locus_tag="PLZ72_07690"
     CDS             complement(1936..3483)
                     /gene="larB"
                     /locus_tag="PLZ72_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNA36212.1"
                     /translation="MTQLDDPPDDVAVGARDRLVEVLRSVGPVVVAFSGGVDSALLAH
                     VALDALGPDRVLAVTARSPSLPSGEWERCRDLAGRWGLPWRSVDTDEMSRPGYVVNDA
                     DRCAWCKDSLLDELDPIIDAGGDLDGATIVLGVNVDDLGDHRPGQEAARARGARFPFV
                     EAGITKAVIRTLARDAGLEVWDRPSQPCLSSRIPYGTPVTMTTLSSIDRAESAVRAMG
                     FSDLRVRHLGDTARVEVPLAELARAGELAGPIAEAVRDAGYRRVVLDLAGLRSGNLND
                     ALSDQARRAATTGLRHTRPDLDRVRRTGLPEAVYAAGKTPAQVVEVVAAMLRDATDPV
                     IVTRTDAGHRDAIDASVCGGELPEPDGRWSDTLTWRHIDAAAENGLGSRRVAVVSGGT
                     SDRPVLDECVGTLTALGAEVRTVVDVGVAGLHRLLDSLDQLTDARVVVALAGMEASLP
                     TVLGGLIHQPIVAVPTSTGYGSSLEGVTALLSLLASCAPGVSVVGIDNGYGAACAALR
                     ILGSGSR"
     gene            3549..4571
                     /locus_tag="PLZ72_07695"
     CDS             3549..4571
                     /locus_tag="PLZ72_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNA36213.1"
                     /translation="MSATSAPVIDVNPGRVDVVGIGNALVDVLSRGTDELVSELGLVK
                     GSMNLIDDARVSELYERMGPGVEVSGGSAANTMAGIASLGGSAHFIGRVRDDQLGAVF
                     AHDMVATGVAYTTSAATDGPSTGCCLIVVTPDAQRTMNTYLGASVFLGPDDVDADVVA
                     SGTVLYLEGYLFDRPDAQEAFRRAAAIAHDAGRTVAVTLSDSFCVERHRDDFLDLVAD
                     HVDLLFANEAEITALYRTDFDAAVEAVRRDGTVAALTRSELGSVIVTGDATIEVPARA
                     VSELVDTTGAGDLYASGFLHGVSRGRDLATCGALGSLAASEVISHMGPRPEVSLAGLA
                     APLLAD"
     gene            complement(4508..5281)
                     /locus_tag="PLZ72_07700"
     CDS             complement(4508..5281)
                     /locus_tag="PLZ72_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36214.1"
                     /translation="MTWFALWYIQTHLILSLIGGPLRRWQQQRPRRLWTAIGALFVLG
                     AAGAPGLSIMMFYVACWVLGYAHHDGRVEAWLRPRWKRVCWLLGPVGAVAFFGFHQRA
                     IVVAAFGVAFLGIFWLSLALGLQPRIEPWLEGRFQRSVTSWFSQRSLTIYLWHGVALW
                     VMVELDLPGALVTRLVGCAVLVVAAVVVFGWLEDVAARRPAQLWPRLPSARASRPNGR
                     LRPAVIDLRARDGSRVPAPQSASSGAANPASDTSGRGPM"
     gene            complement(5278..5658)
                     /locus_tag="PLZ72_07705"
     CDS             complement(5278..5658)
                     /locus_tag="PLZ72_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNA36215.1"
                     /translation="MADSVDTRLKRSIYLDALRAVALVRVVIFHATGSWLVTAFTAMP
                     LMFFIAGTLYAASLDRRATSKVVRDRYRRIMVPYWGYLAAMVALWGALGVLGEITPAN
                     WVGFLMPVVSMAGPKGPGPTRRCT"
     gene            5842..6501
                     /gene="deoC"
                     /locus_tag="PLZ72_07710"
     CDS             5842..6501
                     /gene="deoC"
                     /locus_tag="PLZ72_07710"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNA36216.1"
                     /translation="MPVPVAIEHAALRPEATVAEVLEGAAIAASRGCVAVCVAPAMVH
                     PVVTAMTRPDGGAIAVASVVGFPHGTQLSRTKAVEAAAAVALGAERLVAVADLGAVAD
                     GDLTAVAADVAAVRDAVPSTLIDVVVESALWTPAVLRQVCEAVIDGGADSVTTSSGFH
                     PAGGASDGAVAVLRAAAGDRARVTAAGGITDSATALAMVEAGADTLRLADTFAVLDGL
                     R"
     gene            6501..7628
                     /locus_tag="PLZ72_07715"
     CDS             6501..7628
                     /locus_tag="PLZ72_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36217.1"
                     /translation="MPASGGTVGPDVVVPGSQQVAPAFFLAVTVVAVLVLVGLLVRRH
                     LRARRGSARRRPIGVSAALGVVVLAGAAAWLTRPPPFFVPELGALPTLMPTDNVFRRT
                     ATDLPVSADSGRWIGSISDLELSAGFGGRVVDGVVWGIPYNFVDDATVRRPVAIRLEP
                     RSSFPGPYPITDPAYIESMPTYGFDMHYVGIDPERSEVWELLSTRAWFGRWEADSGAH
                     YSTRSNAYPVGSTIAAGLPLLPGTITYDEVASGEVDHVVLAAIETTATGSWIWPARGT
                     DGRSASPEAPPQGAWLRLRETADLSALGPQATVVADALRTHGLILSDTGPGFALRGTP
                     DRRWDDDDLATLRTLHASDFEVVDPSGIVVDPEQLAVRPPA"
     gene            complement(7641..8462)
                     /locus_tag="PLZ72_07720"
     CDS             complement(7641..8462)
                     /locus_tag="PLZ72_07720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNA36218.1"
                     /translation="MTTTSTGPDDAFARAGRTAGQIRDRLGEHTVAIVLGSGWASAVD
                     GLGPAAGSIPVDDLDGVPPPTAAGHGHTVTSLDVDGVATLVFTGRAHLYEGHGPTAVV
                     HAVRAAVLSGCRVVCLTNASGSLRSDVGVGTPVVISDHLNLTGTSPLLGAEPPGAVGN
                     RFVDLTGLYSPRLRAAAGSDRVEGVYAGVIGPHYETPAEVRMLATMGADLVGMSTVLE
                     AIAARHLGAEVFGVSLVTNLAAGLGPGFDHGSVLAAGSAAVGELSGMLRRVVGAA"
     gene            8598..11420
                     /locus_tag="PLZ72_07725"
     CDS             8598..11420
                     /locus_tag="PLZ72_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNA36219.1"
                     /translation="MRTLERLTQAPGGPSGPRRGLGPVLGVLLGVLSLPILVLLVVSK
                     WGVLIAVLLAALIGLGLWTMKRGFLFIEIVAFLIHFDGVGVGPIRLGRITAVVAFGVV
                     AYKLVVERWRPPAIPTRHWLPIMLLVLYGVLSGVWIEYIGAWFFAFALLGLALAYYAV
                     SALLVDSHEKVMRYLRAFWVGGLFGSMAGVLGLVLGTRSVGFGGDPNYFGMIQATMIP
                     LTVYYRRNARTPREKSWYTLALILVLAGAAGAGSRSGLIGGSIAIVGTLITRPRLSAT
                     RRAKVAVGAVLLAGTAFLIGFVANPNNLSRGFADRGAGRLDMWTATIPLIKDRPLLGH
                     GFGQLGRKVLPSLTTSPGVEELMDQRADVSSHNTILDILGDLGIVGLCIWGSIFGVTI
                     LGFLRPRWRQTKEISTTMFVMMLPIASSSMFLPMINQKIAVSLVGMASALQVPSWGAR
                     WRGFSGIMGSGRSLDVVGPPAGGMAAGVPARTSAADPRTERPDLKWEPVVLARWDVKI
                     SRRFRYLIAAGAFAGLLTFSLVASFLPTTWSASADVILIDMSATQGNNRVAINSTANQ
                     VMDTIATSGAYAQTLKQLSGVDLTVPEVRDRVITTRTGFGAAMTIAFSDTDRRLVEQV
                     QPHVLPAFDRTLQSSREASVAVLEDELRPIYPGEQRFDTSQLYLPLDGEASLGSDQTS
                     RVWVGMIGALFGALSALAWILFQQHRPRVNNDDDLTEAIGLGVWSHVGRPGRRYGASP
                     AQYAQVGTMATEMAAGESPPRRVVITTPTPDRAAGTLATGVAAAIAAEGRRVVLVDAQ
                     TDAPGLTRRLSWPWRSGFVDVMAGAPIDALLRRVDRWRLSSSARRTLGRHGDQMRLLP
                     VGRRPRDVTVIRPEVIDRFGPDVAVVIMAPSLCSGVAVGALLAWADVTVLALVEGRTV
                     TFDAEDAAAPIRTFATAPSGVVMLDV"
     gene            11448..12281
                     /locus_tag="PLZ72_07730"
     CDS             11448..12281
                     /locus_tag="PLZ72_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNA36220.1"
                     /translation="MNDSARTEIEWFDSHCHLDGEEDPATVVADAAAAGVVGMVTVGT
                     DGASSAVALELARSIGRVWATAGVHPHEAEHGIERLQEVVAEAVEAADGRLVAIGECG
                     LDYHYDHSPREAQRRVFAEQVEMAHRFDLPLVIHTREAWSDTFAVLREQTVPDRTVFH
                     CFTGGPEEAMRCVDLGAHLSISGIVSFRNATDLRSAVACVPLDRLMVETDSPYLTPVP
                     FRGRRNRPEYVRRVGEELAAVLGRRPEEVAASTTEAARRFFGMRSGGPVVGDRSPTGD
                     V"
     gene            12639..13472
                     /gene="rsmA"
                     /locus_tag="PLZ72_07735"
     CDS             12639..13472
                     /gene="rsmA"
                     /locus_tag="PLZ72_07735"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNA36221.1"
                     /translation="MLGRTDVDELLARHGLAARRSLGQNFVADPNTVRAIARRAAVGP
                     GDRVVEIGPGLGSLTLALAETGAEVLAVEKDRSLLPVLTEVLAGSSGVCVVQGDATTI
                     EWDHLLGVGPWVLVANLPYNVAVPLTLRVLCRAPQVERLVVMVQREVADRLCASPGGR
                     TIGVPSIKVAWWASARIAVRVGPEVFVPRPRVESAVVELVRHPPPSTRIGPRQLFPLV
                     EQAYRQRRKMLRSTLAGSVSAAQFASADVDATARPEQLGLEEWVRLAEVVALGDRGGA
                     R"
     gene            13469..14176
                     /locus_tag="PLZ72_07740"
     CDS             13469..14176
                     /locus_tag="PLZ72_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNA36222.1"
                     /translation="MIEVVAPAKLTLSLRIRGVRSDGFHEIDAEMVSLDLGDVLEIDL
                     EGDGLLVERDGRWEPGGSDDLVNRSLRLVDRRVGVRLRKVVPAGAGLGGGSADAAAIL
                     RWAGVRDPRRAVHLGADVAFCVVGGRARVRGIGEDIEPLPFEDRTFTLLTPPLHCSTP
                     VVYRRWDDMGGPLGDHGNDLEPAALSAYPELARWRDELENLTGRRPRLAGSGSTWFVE
                     GAYEGPSTVVARTVRGS"
     gene            14368..15930
                     /locus_tag="PLZ72_07745"
     CDS             14368..15930
                     /locus_tag="PLZ72_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36223.1"
                     /translation="MSVAVVLAWQFRFVQDDAFISFRYAKNLVDGNGLVFNPGQPVEG
                     YTNFLWTVLMAVPIALGLDPVVFSQVAGILAMVGTVLVTERLGTQVLRSSVRGTVVAI
                     ALVLQFSFLAYGTGGLETQLQSFLVSSAVLALVPWLGMPGRDGAAAARAGGASALIAM
                     ALLTRLDSAVLLVVPGVVAAVVLVRRRAVAHLAALVVPAAVILGAWFAWKVAYYGAAV
                     PNTLEAKSMGPTSVLVGAAFLFVFVWYSLYTVFLPALVRRARDLVGIPGMWPVVGALL
                     VWVVYLLYVGADFMEFRFMVPAMPFIAVVLIGIIYCCRSVAARWILVSALMLGLPFHA
                     ALFEGQINVDSTRTLEQWVVGPTGWVAMGRRLARDVATVAGSADDPPVIAVSAAGAIP
                     YESDLPTVDMLGLNDPVTAREGLAIPVQTLPKPGHIRMATVEHLRSEGVNLVIGHPMA
                     SDPSDGPYDLASLVETMFLRAGIDVSNLEGVTVVEVPLADGKVLPMLYLKPSRVVDRL
                     VEDRVWRQVPTG"
     gene            complement(16005..>16090)
                     /locus_tag="PLZ72_07750"
     CDS             complement(16005..>16090)
                     /locus_tag="PLZ72_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HNA36224.1"
                     /translation="KRRKRMRKKKHKKLLRRTRHQRRARGK"
BASE COUNT         2145 a         5603 c         5873 g         2469 t
ORIGIN      
        1 cacgccggca taccgggcaa gcggctggtg cgcctcgacg accacaccga ggcggcggtg
       61 accgcggacc tcgtcgcccg ggccgcggcc ggcgaccgcg tggcgctggt cagcgatgcc
      121 ggcctgcccg ggttgtcgga tccgggcgcg gtcctggtag ccgcggcgtg gagcgccggc
      181 gtggcggtcg aggtcgtgcc cggaccgttc gccgccgcgg tgggcctcgt cggcagcggg
      241 atgctggagc cgtccggtcg gttctgcttc gagggattcc tgccccggaa gggcccggcg
      301 aggcgcaccc ggatcgccgc cctggtgggc gaggagcgca cgaccgtcct ttacgaggcc
      361 ccgcaccggc tccgctccac cctggaggac ctgcgcgacg ccctcggcgc ggaccgccgt
      421 gtggcgctgt gccgcgagct gaccaagctc cacgaggaga cctggcgcgg ctcgctcggc
      481 gacgccgtcg cccggtgtgc cgaggtggag ccccgcggcg agttcgtcgt ggtgctcgcg
      541 ggggccgagc cgccgcccga tgccaccgac gacgagctgc gtcgctccct cgatcgtgaa
      601 ctggagaccg gcgccagccg ccgcgacgcc gcggccacgg tcgcggcacg cttcgacgtg
      661 gcccccaacc gggtcaagcg cctgctgagc ggctgagcgg ctgagcgcgt gagggcgccg
      721 gtcaggggag cgtgtcgcgg aacgcggcgg cggcatcggc ggccacgtca cgggcggagc
      781 gccccgacgc ccgggcgatc ccgaccacgt cgtcgtgctc gggcttggct ccgtgggggc
      841 tgaccttgat gcgcaccggg cggccgtcca gctccaccac cgtctcgtgg cggggcagct
      901 cgtgcttggt gaccgaccac tcgcgccacc ccagcgtgcc ggtctcgcgt gcgagcagcc
      961 cgcgcacctc cgggaccagc ggcgggctgc acagcacccg gacctggtga ccgggccgac
     1021 tcttcttcat ggtgaccggc aggacccacg cgtcgtcggc tcccatggcg atcgcggcgt
     1081 cgatgacgtg gccgaggacc tcgggggtga cgtcgtcgac ggttgtctcg atcagcaccg
     1141 tctcgatcag cactgtctcg accccaacct ctcggtccgc ggtcgggtcg gtcggctcga
     1201 acgccaccgc ggtcaccacg ttggcgtgcc cgggcgggtc ccaggagccg gccccccggc
     1261 cgaccgagtg gaccgtcccg gcgggcatcg gtccccagcg gtcgggggag tccaccatcg
     1321 acacgaggag tgcgaccccg gtgggggtgg cggtctcgcc ggctgcgtcg accggcaccg
     1381 tcgggatcct gcgcagcagc tcgagggtgg ccggtgcggg gaccgcaacg gtgccgtgcg
     1441 ccatggcgac ggtgccgacc ccgaggccga ccggcccggc ggacacgtcg ggttggccca
     1501 ggtcggacca cgccgcccag gttccgacga tgtcgaccag ggcgtcgatc gcaccgacct
     1561 cgtggaagtg cacctggtcc atctccacgc cgtggacggc ggcctcggcg cgggcgagca
     1621 gctcgaaggt ccgccgggct ccccggctga tcgcggcggg gaggtcggcg tcggccagcc
     1681 gggcgtcgat ggccgaccag ggtcggtggc gctggccgtc ctccgcgtgg acggtgaccg
     1741 aggtcgcggt caaccccctc cgggtggtgc caccagcgtc gagtgtccac ccgtcgatgc
     1801 cgagcgagcc gaccgcggcg cgcaccgcgt cgagcgaggc gccgaggtcg accagggcgg
     1861 cgagcagcat gtcgccggcg acgccgaccg ccgggttgat ccacagcccg gacggagtcc
     1921 ggtcgccggt tccgctcacc gcgacccgga cccgaggatg cgcagggccg cgcacgccgc
     1981 gccgtagccg ttgtcgatgc cgacgacgct gacccccgga gcgcaggacg cgagcagcga
     2041 cagcagcgcg gtgacgccct cgaggctgga cccgtagccg gtcgaggtgg ggaccgcgac
     2101 gatcggttgg tggatgaggc cgcccaggac cgtgggcagc gacgcctcca tgcccgccag
     2161 cgcgacgacg acccgggcat cggtgagctg gtcgagggag tcgaggagcc ggtgcaggcc
     2221 ggcgaccccg acgtcgacga cggtgcgcac ctcggcgccg agcgcggtca gggtcccgac
     2281 gcactcgtcg agcaccggtc ggtccgaggt gccgccggac acgacggcca cccgtcggga
     2341 cccgagaccg ttctcggcgg cggcgtcgat gtgccgccac gtgagggtgt cggaccaacg
     2401 gccgtcgggc tcgggcagct cgccaccgca gaccgaggcg tcgatcgcgt cgcggtgccc
     2461 ggcgtcggtg cgggtgacga tgaccggatc ggtggcgtcg cgcagcatcg ctgcgacgac
     2521 ctcgacgacc tgggccgggg tcttgcccgc cgcgtagacc gcttcgggca gaccggtccg
     2581 gcgcacccgg tcgaggtcgg ggcgggtgtg tcgcagtccg gtggtggcgg cccgccgggc
     2641 ctggtcgctc agcgcgtcgt tgaggttgcc cgagcgcagg ccggccaggt ccagcacgac
     2701 gcggcggtac ccggcgtcgc ggaccgcctc ggcgatcggt ccggccagct cgccggcccg
     2761 tgccagttcg gcgagcggga cctcgacgcg ggcggtgtcg cccaggtggc ggacccgcag
     2821 gtccgagaac cccatggccc tcaccgcgga ctcggcccgg tcgatcgacg acagcgtcgt
     2881 catcgtcacc ggggtcccgt aggggatgcg cgaggacaaa cacggttggg agggacggtc
     2941 ccacacctcg aggccggcgt cgcgggccag ggtccggatc acggccttgg tgatcccggc
     3001 ttcgacgaag gggaagcggg cgccacgggc gcgagccgcc tcctggccgg gccggtggtc
     3061 gcccaggtcg tcgacgttga ccccgagcac gatcgtggca ccgtcgaggt caccgcccgc
     3121 gtcgatgatc ggatcgagct cgtcgagcaa cgagtccttg caccaggcgc agcgatccgc
     3181 gtcgttgacg acgtagccgg gtcggctcat ctcatcggtg tcgaccgaac gccagggcaa
     3241 cccccaacgg ccggccaggt cccgacagcg ctcccactcg ccgctgggca gcgacgggga
     3301 gcgggccgtg acggccagga cccggtcggg tccgagggcg tcgagggcga catgggccaa
     3361 cagcgccgag tcgaccccgc ccgagaacgc gaccaccacc ggtcccaccg accggagcac
     3421 ctcgaccagt cggtcccggg ctccgaccgc gacgtcgtcg ggaggatcgt cgagctgggt
     3481 cacggaccgg atcgtaccgg cggcaccggg tccgactccc gtggggggtg ccgctagcgt
     3541 cggcggccgt gtctgcaacc tcagcccccg tgatcgacgt caaccccggc cgcgtggacg
     3601 tcgtcggcat cggaaacgcc ctggtcgacg tgctcagccg tgggaccgac gagctcgtct
     3661 ccgagctcgg gctggtcaag ggatcgatga acctgatcga cgacgcgcgt gtctccgagc
     3721 tgtacgagcg gatgggaccc ggcgtcgagg tgtcgggcgg atcggcggcc aacacgatgg
     3781 ccggcatcgc gtcgctcggc ggcagcgccc acttcatcgg acgggtgcgg gacgaccagc
     3841 tcggggcggt gttcgcccac gacatggtcg ccacgggggt cgcctacacc acctcggccg
     3901 cgaccgacgg gccgtcgacg gggtgctgcc tgatcgtcgt cacccccgac gcccagcgca
     3961 cgatgaacac ctacctgggg gcgtcggtgt tcctcggacc cgacgacgtc gacgcggacg
     4021 tcgtggcgtc gggcacggtg ctgtacctcg agggctacct gttcgatcgc cccgacgcgc
     4081 aggaggcctt ccgtcgggcc gccgccatcg cgcacgacgc cggccggacg gtcgcggtga
     4141 ccctgtcgga cagcttctgc gtcgaacgcc accgcgacga cttcctggat ctggtcgccg
     4201 accacgtcga cctcctgttc gccaacgagg ccgagatcac cgctctgtac cgcaccgact
     4261 tcgacgcggc ggtcgaggcc gtgcgtcgcg acggaaccgt cgccgcgctg acgcgctccg
     4321 agctcggctc ggtcatcgtc accggggacg ccacgatcga ggtgcccgcc cgggcggtgt
     4381 cggagctggt cgacaccacc ggcgccggcg acctctacgc ctcgggcttc ctgcacggag
     4441 tcagccgggg ccgggacctg gccacctgcg gcgccctcgg ctccctcgcg gcctcggagg
     4501 tcatctctca catgggaccg cgacccgagg tgtcgctggc cgggttggcc gcaccgctgc
     4561 tcgccgactg aggcgccggc acccgggacc cgtcgcgggc ccgcaggtcg atgaccgcgg
     4621 ggcggagccg accgttcggg cgcgacgccc gggccgaggg gagccgtggc cacagctggg
     4681 ccgggcgtcg agccgcgacg tcctccaacc agccgaagac cacgaccgcg gccacgacga
     4741 gcacggcaca gccgaccagg cgcgtcacga gggcgcccgg caggtcgagc tcgaccatca
     4801 cccacagggc gacgccgtgc cagaggtaga tggtcagcga gcgctggctg aaccagctgg
     4861 tcaccgaacg ctggaaccgg ccctcgagcc acggctcgat ccggggctgg aggccgagcg
     4921 cgagcgacaa ccagaagatg cccaggaacg ccacgccgaa cgcggcgacc acgatcgccc
     4981 gctggtggaa gccgaagaac gcgaccgccc cgaccggacc cagcagccag cacacccgct
     5041 tccagcgggg tcgcagccac gcctccaccc ggccgtcgtg gtgggcgtag ccgagcaccc
     5101 agcaggccac gtagaacatc atgatcgaca ggcccggtgc gccggccgcg cccagcacga
     5161 acagggcgcc gatcgccgtc cagagtcggc gcggtcgctg ctgctgccaa cggcgcaacg
     5221 gcccgccgat cagcgacagg atcaggtgcg tctggatgta ccagagggcg aaccacgtca
     5281 ggtgcagcgc cgtgtcggcc cggggccctt cggtcccgcc atcgacacga ccggcatgag
     5341 gaagcccacc cagttggccg gtgtgatctc accgagcacc ccgagcgcac cccacagggc
     5401 gaccatcgcc gccagataac cccagtacgg caccatgatc cggcggtagc ggtcccggac
     5461 gaccttggac gtggcccgac ggtcgagcga cgccgcgtag agcgtgccgg cgatgaagaa
     5521 catcaacggc atcgcggtga aggcggtgac cagccaggac ccggtggcgt ggaagatcac
     5581 cacgcgcacg agcgcgaccg cccgcagcgc atccaggtag atgctccgct tcaatcgcgt
     5641 gtcgacggag tccgccaacg atctcgcccc ttgtgcccct tgcccctgag tgacggtgac
     5701 ccgaacccca cacccggtcc ccgtcggcgc agaccctatg aaatccgcca caccggatca
     5761 atggctgagc cggatttccg tgggtggatc tccgatgccc ggtggcgaga ccccggtccc
     5821 ggggccggta ctctcgggtt ggtgccggtc cccgtcgcca tcgagcacgc cgccctgcga
     5881 cccgaggcga cggtggccga ggtcctcgag ggcgccgcga tcgcggcctc tcggggctgc
     5941 gtggcggtgt gcgtggcgcc ggccatggtc catcccgtcg tcaccgccat gacccgaccc
     6001 gacgggggtg cgatcgcggt cgcgtcggtc gtcgggttcc cccacgggac ccagctgtca
     6061 cgcaccaagg cggtcgaggc cgcggctgcg gtcgccctcg gcgcggagcg cctggtagcg
     6121 gtcgccgacc tgggagcggt cgcggatggc gacctgacgg cagtggcggc cgatgtggcg
     6181 gcggtccgcg acgcggtgcc gtccaccctg atcgacgtgg tcgtcgagtc agcgctgtgg
     6241 acgcccgcgg tgctgcgcca ggtctgtgag gcggtgatcg acggcggggc ggactcggtg
     6301 accacgtcga gcgggttcca cccggccggt ggggcgagtg acggagccgt ggccgtgctg
     6361 cgggccgcgg ccggggaccg ggcgcgggtc accgcggcgg gcggcatcac cgacagcgcg
     6421 acggccctgg cgatggtcga ggccggcgcc gacacgctgc gcctggcgga cacgttcgcg
     6481 gtcctcgacg gcctccgctg atgccggcgt caggggggac ggtcggcccc gacgtggtcg
     6541 tccccggatc ccagcaggtc gcgcccgcgt tcttcctggc cgtgacggtc gtggcggtcc
     6601 tggtcctcgt cggtctgctc gttcgtcgcc acctccgggc ccgtcgcgga tctgcgaggc
     6661 gtcgaccgat cggggtgtcc gccgcgctgg gggtcgtggt gctcgccggc gccgcggcgt
     6721 ggctcacgcg tccgccgccg ttcttcgtgc ccgagctcgg tgcgctcccg acgctcatgc
     6781 cgaccgacaa cgtcttccgg cgcaccgcga ccgacctccc ggtctccgcc gacagcgggc
     6841 gttggatcgg ttcgatctcg gacctggagc tctccgccgg cttcgggggc cgggtcgtcg
     6901 acggcgtggt gtgggggatc ccctacaact tcgtcgacga cgccaccgtc cgtcgcccgg
     6961 tcgccatccg gctggagcct cgcagcagct tcccgggccc gtacccgatc accgatccgg
     7021 cctacatcga gtcgatgccg acctacggct tcgacatgca ctacgtgggc atcgaccccg
     7081 agcgctccga ggtgtgggag ctgttgagca cccgggcctg gttcggtcgg tgggaggccg
     7141 actccggcgc gcactactcg acccggtcga acgcctaccc cgtcgggtcg accatcgccg
     7201 cggggctgcc gttgctgccc ggcacgatca cctacgacga ggtcgcgtcc ggcgaggtcg
     7261 accacgtcgt gctcgccgcg atcgagacga ccgcgaccgg cagctggatc tggcccgccc
     7321 gcggcaccga cggccgatcg gcgtcgcccg aggcgccacc gcagggagcc tggctgcgtc
     7381 tgcgcgagac agcggacctg tcggcgctcg gcccgcaggc gacggtggtg gccgacgcgc
     7441 tccggaccca cgggttgatc ctgtccgaca ccggtcccgg cttcgcgttg cggggcacgc
     7501 cggaccgacg ctgggacgac gacgacctgg cgacgctgcg cacgctgcac gcgtcggact
     7561 tcgaggtggt cgacccgtcc gggatcgtgg tcgacccaga acagctcgcc gtccgccctc
     7621 cggcctgatc ggccggcggt tcaggcagct cccaccaccc gacgcagcat cccgctcagc
     7681 tcacccactg ccgcgctccc cgcggcgagc accgaaccgt ggtcgaagcc ggggcccagc
     7741 ccggcggcca ggttggtgac cagcgacacg ccgaacacct cggcgccgag gtggcgcgcc
     7801 gcgatggcct cgaggaccgt cgacatcccg accaggtcgg cccccatcgt cgcgagcatg
     7861 cggacctcgg ccggggtctc gtagtgcggt ccgatcaccc ccgcgtacac cccctccacc
     7921 cggtcggacc ctgcggcggc gcgcagacgg gggctgtaga gaccggtcag gtcgacgaac
     7981 cggtttccca ccgctcccgg cggctccgcg ccgagaagcg ggctcgtccc ggtcaggttc
     8041 aggtggtcgc tgatcaccac cggcgtcccg acgccgacgt cgctccgcag ggatccggac
     8101 gcgttggtga ggcacacgac ccgacatccc gacagcaccg cggcgcgcac cgcgtggacg
     8161 accgcggtcg gtccgtgacc ctcgtagagg tgggcccggc cggtgaacac gagggtcgcg
     8221 acgccgtcga cgtcgagcga ggtgaccgtg tggccgtgac cggccgcggt cggcggcggg
     8281 accccgtcga ggtcgtcgac ggggatcgag ccggcggccg gtccgaggcc gtcgaccgcg
     8341 gaggcccacc cggagccgag cacgatcgcc acggtgtgct caccgagtcg gtcgcggatc
     8401 tggccggcgg tgcgcccggc ccgggcgaag gcgtcgtcgg ggcccgtgct cgtcgtggtc
     8461 atcgccggat ccttgcactt gcacctgcgg gcacccgcta cgggtcggtg gaccagcccc
     8521 ggtagcctgg atccgcggat cggccgggtg tgcgaccgcg gccggcaccg gggacgtccc
     8581 gggcccacgg gaggatcgtg cgcacactgg aacgtctgac tcaggccccc ggcggaccgt
     8641 cgggtccccg tcgcggcctc ggaccggtcc tcggcgtgct cctcggggtc ctgtcgctgc
     8701 cgatcctggt tctcctggtc gtctccaagt ggggcgtgct catcgccgtg ctgctggccg
     8761 cgctgatcgg cctcggtctg tggacgatga agcggggctt cctgttcatc gagatcgtgg
     8821 cgttcctgat ccacttcgac ggcgttggcg tgggaccgat ccgcctggga cgcatcacgg
     8881 cggttgtcgc cttcggcgta gtggcgtaca agctggtggt ggagcgttgg cgtccacccg
     8941 caatacccac ccggcattgg ctgccgatca tgctcctcgt cctctatggc gtcctcagtg
     9001 gggtctggat cgagtacatc ggcgcctggt tcttcgcgtt cgccctcctc ggactggcgc
     9061 tcgcttacta cgcggtctct gccctcctcg tcgacagcca cgagaaggtc atgcggtacc
     9121 tccgggcgtt ctgggtcggt gggctcttcg gctcgatggc gggggtgctg ggactcgtcc
     9181 tgggcacacg gtccgtcgga tttggtgggg atcccaacta cttcgggatg atccaggcga
     9241 cgatgatccc gctgactgtc tactaccggc gcaacgcgcg gactccgagg gagaagtcct
     9301 ggtacaccct tgctctgatc ctggtcctgg ctggcgcagc cggggcggga tcacgttccg
     9361 gcctgatcgg ggggtccatc gcgatcgtcg gcacgctgat cacccgacct cgcctgagcg
     9421 cgacacgtcg cgccaaggtc gcggtcggag cggtcctgct cgccgggacg gcgttcctca
     9481 tcggcttcgt ggccaacccc aacaacctca gccgggggtt cgccgaccga ggggccggac
     9541 gcctggacat gtggacggcg acgattcctt tgatcaagga ccgacccctg ctcggacacg
     9601 gcttcggtca gctcgggcgg aaggtcctcc ccagcctcac gacgtccccg ggggtcgagg
     9661 agctgatgga ccagcgggct gacgtgtcgt cgcacaacac catcttggac atcctcggcg
     9721 acctcggaat tgtcgggttg tgcatctggg gttcgatctt cggcgtcacg atcctcggat
     9781 tcctccgacc gaggtggcgt cagaccaagg agatctccac gaccatgttc gtgatgatgc
     9841 tcccgatcgc gtcgagttcg atgttcctcc cgatgatcaa ccagaagatc gcggtgtcgc
     9901 tggtcgggat ggcgagcgct ctgcaggttc cgtcgtgggg agcccggtgg cgaggtttct
     9961 cgggcatcat ggggtccggt cgctccctcg acgtggtggg tccacctgca ggtggaatgg
    10021 ccgcgggcgt cccggcccgc acctccgcgg ccgatccgag gaccgagcgc cccgatctca
    10081 agtgggagcc ggtcgtgctc gctcgctggg acgtcaagat ctcgcggagg ttccgctacc
    10141 tcatcgccgc gggagccttc gccgggctgt tgacgttctc cctcgtcgcg tcgttcctcc
    10201 cgacgacctg gagtgcgtcg gccgacgtga tcctcatcga catgtccgcc acccagggca
    10261 acaaccgggt cgccatcaac tcgacggcga accaggtgat ggacaccatt gccacgtccg
    10321 gggcctacgc ccagacgctg aagcagctct cgggagtgga tctgaccgta ccggaggtcc
    10381 gcgaccgggt tatcacgacg cgcaccgggt tcggagctgc catgaccatc gcgttctcag
    10441 acaccgaccg acgcctggtc gagcaggtgc aaccccatgt cctgccggcg ttcgaccgaa
    10501 ccctgcagtc ctcgagggag gcttcggtcg ctgtcctcga ggacgagctg agaccgattt
    10561 acccgggcga gcagcgcttc gacacctccc agctgtacct gccgctcgat ggggaggcgt
    10621 cgctgggttc ggatcagacc tccagagtct gggtggggat gatcggagcg ctgttcggag
    10681 ccctcagtgc tctggcctgg atcctattcc agcagcaccg gccgcgggtc aacaacgacg
    10741 acgatctgac cgaggcgatc gggctcggtg tgtggtccca tgtcggccgg ccggggcgcc
    10801 gttacggagc gagccccgcc cagtacgcac aggtggggac gatggccacc gagatggccg
    10861 cgggagagtc cccaccccgg cgtgtcgtga tcaccacacc gaccccggat cgagcagccg
    10921 gaaccctcgc caccggagtc gcggcggcga tcgccgccga aggacgacgt gtcgtcctgg
    10981 tcgacgccca gacggacgct cccggtctga cgcggcggct gtcgtggccg tggcgctccg
    11041 ggttcgtcga cgtcatggcc ggcgcgccga tcgacgcgct tctccggagg gtcgaccggt
    11101 ggcgcctgtc gtcctcggcg cgaaggactc tgggtcggca cggcgaccag atgcggctcc
    11161 tgcccgtggg ccgacgaccc cgcgacgtca cggttatccg cccggaggtg atcgaccggt
    11221 tcgggccgga cgtggcggtg gtgatcatgg ctccctcgct ctgctccggg gttgcggtgg
    11281 gagcgttgct ggcgtgggcg gatgtcacgg ttctcgccct ggtcgagggt cgtaccgtca
    11341 cgttcgatgc cgaggacgcg gcggcgccga tccgcacctt cgccacggcc ccgagcggcg
    11401 tggtcatgct cgatgtgtga gagcctggtg gacgtgaggt acgtcgcgtg aacgactccg
    11461 ctcgtaccga gatcgagtgg ttcgacagcc actgccacct ggacggcgag gaggacccgg
    11521 cgacggtcgt ggccgacgcg gccgcggccg gggtggtggg gatggtcacc gtcggcaccg
    11581 atggtgcgag ttcggcggtg gcgctcgaac tcgctcgatc gatcggacgt gtgtgggcga
    11641 cggccggtgt ccatccgcac gaggcagagc acggtatcga gcgcctgcag gaggtggtgg
    11701 ctgaggcggt cgaggccgcg gacggtcgac tggtcgcgat cggtgagtgc ggcctggact
    11761 accactacga ccactcaccg cgagaggctc agaggcgggt gttcgccgag caggtcgaga
    11821 tggcccaccg gttcgacctg ccactggtga tccacacgcg cgaggcgtgg agcgacacgt
    11881 tcgccgtgtt gcgggagcag accgtgccgg atcgaacggt cttccattgc ttcaccggcg
    11941 gaccggagga ggcgatgcgg tgcgtggatc tcggggccca tctgtcgatt tcagggatcg
    12001 tgagcttcag gaacgcgacc gacctccgct ccgcggtcgc atgcgtgccg ctggacaggc
    12061 tgatggtcga gacggactcg ccctatctca ccccggttcc gttccgaggc cgtcgcaatc
    12121 ggcccgagta cgtgcgccgg gtgggcgagg agctcgcagc ggtactcggt cggcggcccg
    12181 aggaggtcgc cgcgtcgacg acggaggcgg cccgacggtt cttcgggatg cggtccggcg
    12241 gaccggtcgt gggggaccgt tcgccgacgg gtgacgtgtg agcaccccgt cggtccggct
    12301 gcggatcgcg ctggtagcga ccgtgtgcct tgtggccgtc ctgatcgggt cgtccttgac
    12361 ccgatcggac gaccagccgt cgacctcgtc cacgccgggt cggtcgagcg cgtcgtcccc
    12421 gacggagtcg agcgccacat cggccgccgt tccgagggcg acgattgctc ccgctcggat
    12481 cacggtcctt cccgactggt accggaagca gtcgagccgg tacgcggaac gacggccgtc
    12541 gacggtgcca ccggacgtcc cgacgacgac cgagccccgg ccggtgatga gcaacggatt
    12601 gggtcggccg agcttcacgg tggcggagcc gggctgagat gctgggacgg accgacgtcg
    12661 acgaactcct ggcgcggcac ggcctggcgg cgcggcggtc gctcggtcag aacttcgtcg
    12721 ccgatccgaa caccgtccgg gccatcgctc ggcgtgctgc ggtcggtccg ggcgaccgcg
    12781 tcgtcgagat cggtcccgga ctgggctccc tgacgctggc gctcgctgag accggggccg
    12841 aggtcctcgc cgtcgagaag gaccggtcgc tccttcccgt gctgaccgag gttctcgccg
    12901 ggtcgtcggg tgtgtgcgtg gtccagggcg atgcgacgac gatcgaatgg gaccacctgc
    12961 tcggagtcgg tccatgggtc ctcgtggcga acctcccgta caacgtggcg gtaccgctga
    13021 cgttgcgggt gctgtgccgg gcaccccagg tcgaacgact cgtcgtgatg gtccaacgag
    13081 aggtcgcgga ccgcttgtgt gcctcaccgg gtggtcggac gatcggtgtt ccgtccatca
    13141 aggtggcgtg gtgggcttcg gcccggatcg ctgtccgcgt cgggcccgag gtgttcgtgc
    13201 cccgtccacg ggtcgagtcc gcagtggtcg agctcgtccg acatccgcca ccgtcgaccc
    13261 ggatcggacc ccgccagctc ttcccgctcg tcgagcaggc gtaccgacag cgccgcaaga
    13321 tgctgcgctc gacgctggcc ggatccgtct ctgccgccca gttcgcctct gccgacgtcg
    13381 acgcgacggc gcgcccggag cagctcgggc tcgaggagtg ggtgcgcctc gccgaggtcg
    13441 tcgcgctcgg cgatcgcgga ggcgcgcggt gatcgaggtc gtcgctccgg cgaagctgac
    13501 gttgtcgcta cggatccgcg gggtccgatc cgacgggttc cacgagatcg acgccgagat
    13561 ggtctcgctc gacctcggcg acgtactcga gatcgatctc gagggcgacg gtctcctggt
    13621 cgaacgtgac gggcggtggg aaccgggggg atccgacgat ctggtgaacc ggtcgctccg
    13681 cctggtcgac cgtcgggtgg gcgtacggct gcgtaaggtc gttcccgctg gagcgggcct
    13741 cgggggcggc agtgccgacg ccgcggcgat cctgcggtgg gcgggcgtgc gggatccgcg
    13801 acgggcggtc cacctcggtg cggacgtcgc gttctgcgtc gtcggcggtc gggcccgggt
    13861 ccgcggaatc ggggaggaca tcgagccact gcccttcgag gaccggacct tcacgttgct
    13921 gaccccacca ctccactgct cgaccccggt cgtctaccgg cgttgggacg acatgggagg
    13981 accgctcggc gatcacggca acgatctgga gcccgcggcg ctcagcgcct accctgagct
    14041 cgcccggtgg cgagacgagc tggagaacct gacaggacgc cgtccccgtc tggcaggcag
    14101 tgggtccacg tggttcgtgg agggggcgta cgagggaccc tccacggtgg tggcccggac
    14161 ggtgcggggg tcgtgacgga gcgcggcctc cgtggcggag tcggcgggga cgaggtcgga
    14221 tcgaccgaaa ggaacaccgt atcgatggcg atggatgtga cgatcgcgga gcgagtcgtt
    14281 ccgcaggcgg aggtgcagag gcagccgctg acatcgatcg aggcggcgct gggtcgggtt
    14341 ccctcggcgt ggcgcagcgt tctcgtcgtg tcggtcgctg tggtgctggc gtggcagttc
    14401 cggttcgtgc aggacgatgc tttcatttcg ttccgatacg cgaagaactt ggttgacggc
    14461 aacggcctgg tcttcaaccc tgggcagccg gtcgagggat acacgaactt cctgtggacc
    14521 gtcctcatgg cggtgccgat cgccctgggg ctggatcccg tcgtcttctc ccaggtcgcc
    14581 ggcatcctgg cgatggtcgg cacggtgctc gtcacggagc gcctcggcac ccaggtgctc
    14641 cgatcgtccg tacgagggac ggtggtggcc atcgccctgg tgctgcagtt ctccttcctg
    14701 gcctacggga ccggcgggct cgagacccag ctccagtcct tcttggtctc gtcagcggtg
    14761 ctcgcgctcg ttccgtggct cgggatgccg ggtcgggatg gggcggccgc ggcccgagcg
    14821 ggcggggcat cggcgctgat tgccatggcg ctgctcaccc gactggactc ggccgtgctc
    14881 ctcgtggttc ccggtgtcgt cgccgcggtg gtgctggttc ggcgtcgtgc cgtcgcccac
    14941 ctggcggcgt tggtggtccc cgccgcggtg atcctcggag cttggttcgc gtggaaggtc
    15001 gcttactacg gcgccgcggt acccaacacc ctcgaggcga agtcgatggg accgacgtcg
    15061 gtgctcgtgg gcgcagcgtt cctgttcgtg ttcgtctggt acagcctcta caccgtcttc
    15121 ctccctgcgc tggtgcggcg ggcccgggac ctcgtcggga tccccgggat gtggcccgtc
    15181 gtgggagcgc tcctcgtctg ggtcgtgtac ttgttgtacg tcggtgcgga cttcatggag
    15241 ttccgcttca tggtgccggc gatgccgttc atcgctgtgg tcctgatcgg gatcatctac
    15301 tgctgccggt cggtcgcggc acgatggatc ctcgtctcgg ctctcatgct cggattgccc
    15361 ttccacgcag cgctgttcga aggtcagatc aacgtcgact cgacccggac gctcgagcag
    15421 tgggtcgttg gaccgaccgg ttgggtggcc atggggcgac gactcgcccg cgacgtggcg
    15481 acggtggctg gatcggccga cgatcccccg gtgatcgcgg tgagtgctgc cggagcgatc
    15541 ccgtacgagt ccgacctgcc gacggtcgac atgctggggc tcaacgatcc ggtgaccgca
    15601 cgggagggcc tggccatccc ggtgcagaca ctccccaaac cggggcacat ccggatggcg
    15661 accgtggagc acctccgctc ggagggggtg aatctcgtga tcggccatcc gatggcatcc
    15721 gatccctccg acggtcccta cgatctggcg tcgctggtcg aaacgatgtt cctccgtgcc
    15781 ggcatcgacg tgtccaacct cgaaggcgtg accgtcgtgg aggtcccgct cgccgatggc
    15841 aaagtgctgc cgatgctgta cctgaagcct tcgcgggtcg tcgaccgact ggtggaggac
    15901 cgcgtctgga ggcaggtgcc gacaggctga ggctgcaccc atcggtcgga acaggatcag
    15961 gcgcgggacc agtcgcgtct cagatgtggg gacccggtcc ggacctactt gccgcgggcg
    16021 cggcgttggt gacgagtccg ccgcagcagc ttcttgtgct tcttcttgcg catgcgctta
    16081 cggcgcttct
//
LOCUS       DAOQOD010000061        16015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1292_length_16015_cov_11.323684, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000061 DAOQOD010000000
VERSION     DAOQOD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 16015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16015
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_16015_cov_11.323684"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLZ72_07755"
     CDS             complement(<1..1013)
                     /locus_tag="PLZ72_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36225.1"
                     /translation="MDPAGRNRSDHRSPGAPSASETWSDGASDLGILLAGLTGGAGVI
                     HLVMVPAHAGGGSWIDPVGFAVVGWLQIALAVVFLLRRGTRGVAVATAALNAAALAVW
                     IWARTVGLPFGSHAGVVEQVGLIDGVCAALEAAAVVVAVVMAWAPRRLRVGVLVPSIA
                     AVAVLGLVTVGLVSTGSDGEGAAGHSHGTEGTAADAAEHRTQMAAIDDERCDKGFNPK
                     SYWTEASALGVDTYGGGTMAMAPPAPTSGSPDMQQGRGSIGLDRLVSATARSDSGAEL
                     DAAALVVALAGATDEDYRDWRAWMVKRNAVSAGGAAADGGHAHGSAAAPASASSAAPD
                     DNGG"
     gene            1268..1909
                     /locus_tag="PLZ72_07760"
     CDS             1268..1909
                     /locus_tag="PLZ72_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36226.1"
                     /translation="MTDRPHQLTRLTLGDAADAWRRAGFAVDANGSFRLGRTDVVALG
                     TGRPGVRGWALDGIDHPLDGLATVDGHDDDAPDRSPSSHPNGITRLDHVVVSTGDCER
                     TTAAFSSAGLDLRGTRSTTAFGSPVRQSFFWAGDVILELVGPDDGEPTTDEPTSILGL
                     AMVADDLDATAEWLGDRVGTPKDAVQPGRRIATLRHRDLGLGLPVAVMTPHRR"
     gene            1987..3786
                     /locus_tag="PLZ72_07765"
     CDS             1987..3786
                     /locus_tag="PLZ72_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNA36227.1"
                     /translation="MADYRAPVKDALFTLRHIGMMEDLSKTERYAHADPETVAAVLEE
                     QGRFMQEVFAPANAIGDRVGLKWSPDGVETPAEFRAAYAKFVEAGWQGLNADPEYGGA
                     GFPESVYACASEFETAANGALTMAPGLTTGAIECLQHWGTEEQKEVYLRKLITGEWTG
                     TMNLTEPQAGSDVGLCTTKAVPQGDGTYLITGTKIFISFGEHDMADNIVHLVLARLPE
                     APPGTKGISLFIVPKFMVGDDGSLGERNDVRCVSIEHKMGIHASPTCVMSYGDDGGAV
                     GYLVGEENQGMRAMFTMMNSARIAVGIQGAALGDAAYQKALAYSQERRQGREIGGDSK
                     EPAFIIAHPDVRRMLLFMRSHVEAMRALIVYNAAALDLSRALDGDEATAWGEIAELLT
                     PVTKAWCTDVGMEVTSTAVQVFGGMGYIEESGVAQHFRDMRIAPIYEGTNGIQAMDLV
                     GRKLGLRMGGVVNDLLDRMTATVAELDAAGESFASMATELRAGIDTLSDVTSWIMTSG
                     LADPKEALAGATPYLRIFGIVTGGWLMARQALAARAELDAGSSDSAYLEAKIVTARFY
                     AEQQLPQAAGWVASVKGGAAVLYDVDADQLASV"
     gene            3837..4442
                     /locus_tag="PLZ72_07770"
     CDS             3837..4442
                     /locus_tag="PLZ72_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36228.1"
                     /translation="MLDHVFTDAIGALRDALEKALLERQAFEERFQSDVLLGDLTWET
                     SYGLPGEGLPPRVRADLTLEWPTWSQAAYRSWYIEEELPELPRIDIEVVLRIQRLVSP
                     PDPRKVLDALPLESPAIGGERLHRSGPTVEAVSDEDLTETEHAIEISYEGSYELDEAT
                     LADGSTLDDHFSAMGGWISSTLVRLGDLNFEFLPPLEKDDS"
     gene            4520..5299
                     /locus_tag="PLZ72_07775"
     CDS             4520..5299
                     /locus_tag="PLZ72_07775"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNA36229.1"
                     /translation="MTESDVLQVERSDGYAVVTLDRANARNALNATLLAGLTSTMAEL
                     DSDGSVRAIVLTGADPAFCAGLDLKELAASPERLAGGFGDRSELRRPFPDMSTPVIGA
                     VNGVAITGGFELALACDFLIASEHARFADTHARVGIMPGWGLTVLLPQAVGLRRAREL
                     SATGNFLDAETAYDWGLVNHVVAHEDLLPVARKLAADIAGNDPDGVRQILSTYTAGSL
                     LTAADALDLEQRHSREWLARRDAGDLDERRRAVTERGRTQL"
     gene            complement(5357..6196)
                     /locus_tag="PLZ72_07780"
     CDS             complement(5357..6196)
                     /locus_tag="PLZ72_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA36230.1"
                     /translation="MHECFDVSISDHVAHLQLSRPDAYNSMIPSFWSELPQIVGEIDA
                     AGSARAIVISSTGKHFCAGMDLSVFTGGGGLAGSPGVSEEGRRRAHLWNLVQHLQDSF
                     TALETARMPVLAAIQGGCIGGAVDMVTAADMRYCSADAFFCVQEINIGMTADVGTLQR
                     LPKIIPEGVARELAYTGDRMPAQRAYECGLVNQVFEDHQSLVDGTLEIAARIASKSPL
                     AIWGTKEMVNYTRDHSVADSLRYMAGWQAGMFQPGDMLEEFGAKADKRPAEFEPIPPR
                     PQI"
     assembly_gap    6418..6517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6518..8686
                     /locus_tag="PLZ72_07785"
     CDS             <6518..8686
                     /locus_tag="PLZ72_07785"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNA36231.1"
                     /translation="AVTQFESTHARRAFPCFDEPDMKAVFAVSLVVPDHLLAASNASE
                     VDRTDLGDGTVRVTFADTMVMSTYLVALVVGPLEVTDTRMIDGRDRPIPLRVIHPAGS
                     DGLTPFALEVADAGIRLLEDYYDIAYPGDKIDLVAVPDFAFGAMENLGLITFREIALL
                     VDPDEATQPELQRVADVINHELAHMWFGDLVTMRWWEGIWLNEAFATFMEVAASDAFR
                     PEWDTWTGFGLARAAAFDTDALASTRPIEFPVHSPADAEAMFDVLTYEKGASVLRMLE
                     RYLGAERFRDGIRHYLRTHAYGNTATTDLWDALEEVTGEPVRRIMDAWILSGGHPVVT
                     ARLVDDGIELDQTAAARSGDPSLGERWPVPLVVTATVDGSTRHERVLLEGPTVVDLGG
                     APIELRLNTGGDGFFRSELPASLRTADAVDPDSTPLERFVLVDDTWAALLRGTLTAAE
                     VEQLLRLVAWRETDPSVWRRIAQAVRELRRLEGWDGAVRAPRLAQDVAALPRSRATEV
                     ISGVDESGRERWRDLRGILTVVLGVAGNDGDIRDLARDIVHTDPERLRERRDVDPALG
                     AAALDVVAATGDRADHDLIEQRWRTAANPQDTIRHLYALADTPVPECFEKTLDLALGE
                     VRAQDSGYLLRRALANPRHAGRAVEFVSRRWDDVQAKVPSSALVRMLEGVRSITDADL
                     AADVVAFAEAHPLPSGSRVLAQHLERMAVNVTAAQRLRSG"
     gene            8726..9931
                     /locus_tag="PLZ72_07790"
     CDS             8726..9931
                     /locus_tag="PLZ72_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA36232.1"
                     /translation="MSGPPAPGGHRGRTVEHIARPRTAGIGLDVFAVEDRSAQAVCSS
                     LEPGRFTVSVDGPRRRRPLTVEHPGGPVAWELAGLTPGRRHRVTVRTADGRTVARRAF
                     RTLRPPPGEELFRFATLNDLHLGREDKRGTDRLGTRPGARTPQDMARDALGDALAWGA
                     STIVVKGDVCDQSFDRYWDQALSLFAAVPVPVWMLPGNHDTGTRRAVEPDVAARLRGL
                     RMIRGVEHLDVDGLRIVLVESGIPDGGWGDIARHRHDVADLVADARAAGTGTFIGTHH
                     QPQRFRTPLYWPHGTPGPNAGEFARTVRAADAGVMVSSGHTHRCRRRDVAGVPWTEVA
                     STHHFPATWAGYRVFEGGLMQVVRRTAVPGALAWSETTRDALGGIWALWATGTLSDRS
                     FALDWRRLR"
     gene            complement(9924..11129)
                     /locus_tag="PLZ72_07795"
     CDS             complement(9924..11129)
                     /locus_tag="PLZ72_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNA36233.1"
                     /translation="MITDDLLAAAQGVLPDVVELRRDLHVHPELGNHLPRTQGRVLEA
                     LDGLGLEISTGTALSSVVADLVGGAGDGPTILLRGDMDALPMPEDTGLDFASVVDGAM
                     HACGHDAHTAMLVGAATVLAGRRDRLAGRVRFMFQPGEEGFGGATDMIDEGVLDGVDA
                     AFALHVAPNMFSDFVIHRSGAALASADVVKVVVTGRGGHASSPHWATDPVPVACEIVL
                     ALQSMITRTVNAFDPAVLTIAQLDAGTTDNVIPESAALTGTLRAVSEHTRHAVWASID
                     QVAQGVAAAHGCTATVEIVEGYPVTVNDAAFTRWASGVAAEVVGADHVVEMPSPVMGA
                     EDFSYVLREVPGAMFFLGVCPPENPNPFEAPSCHSNRMVLNEDGMATGVAVHVGVATA
                     FLDARGQIT"
     gene            complement(11195..11827)
                     /locus_tag="PLZ72_07800"
     CDS             complement(11195..11827)
                     /locus_tag="PLZ72_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNA36234.1"
                     /translation="MTTTFDVLIGLTLVVAVADWIAVGTDRRRAEYVLKPLTMVVLIG
                     AALAADPVSSGAARGWMVAGLLCSLAGDVFLMLEDHFVEGLASFLVGHVAYVVGLWNL
                     GVDLPRFAVGVVVVAVLMAVIGAPIVSGARRQDPRLAVPVAAYITVISLMVASAVGTG
                     IWIAVLGAALFYVSDGVIGLTRFVRDFPQSRLVVMTTYHLGQIGLVLALV"
     gene            complement(11824..13023)
                     /locus_tag="PLZ72_07805"
     CDS             complement(11824..13023)
                     /locus_tag="PLZ72_07805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNA36235.1"
                     /translation="MTTYLVVGASLAGLSAARTLRRGDPTATIVVVDPSAELPSDRPP
                     LSKQVLAGEWEPDRAQQPSAPEVPDLDLDLRLGVHAVALDVATRTVTLSDGTEQAADG
                     IVIATGATPRRLPGAPPGGVHVLRDMADSLALRRELDAGPARVAVIGAGFIGAEVAAT
                     CRGRGHEVTMIEAAPVPLPRALPAPIGSFVADLHRRHGVDVRLGAGVDRLDADDSGRV
                     RAVRLADGSSVDAEVVVVGIGVTPSTGWLGGSGLDLSDGIVCDSTGLAAPGITAAGDV
                     ASWFNEHFGERMRVEHWEHAIEHGEAAATRLLGGDPAAVAFASVPWFWSDQYDAKIQM
                     AGRPDGDDELHVVDGTIDDGRFVVAFRRGERCTGVLGVNRPRLVMQARMRLRESLAWE
                     PVAELFR"
     gene            complement(13102..13293)
                     /locus_tag="PLZ72_07810"
     CDS             complement(13102..13293)
                     /locus_tag="PLZ72_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNA36236.1"
                     /translation="MKVVVDYDLCESNAVCMGIAPEVFEVREDDLLYVLQEEPSEELR
                     EKVTEAARRCPKQAITVEG"
     gene            complement(13374..14129)
                     /locus_tag="PLZ72_07815"
     CDS             complement(13374..14129)
                     /locus_tag="PLZ72_07815"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36237.1"
                     /translation="MTDLDGRVVVITGGNGGIGLGMAHGVAAAGAAVAVWGRDEAKND
                     AAVEALRAAGAVASSVVVDVADEAAVDAAMAQTLAVHGRVDTMIANAGIGGGAPFVDQ
                     TLEQWRRVMAVNLDGAFLCFRAATRHLLERGEGGSLIGVSSTSAIHGAPANQAYSCSK
                     TAMIALIRGLAVELARHGVRVNGIMPGWVETDMTAPLKGWEKFMANTTARTPVRRWGV
                     PSDFAEAAAFLADPTLTFHTGDLLVIDGGYSIF"
     gene            14230..14829
                     /locus_tag="PLZ72_07820"
     CDS             14230..14829
                     /locus_tag="PLZ72_07820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA36238.1"
                     /translation="MSGSPLRIATARDVDALSQVLARGFGDDPIWRWMAPTGTRWPDR
                     LGPIFRHLLRPPIGFGATWTTDDLEGTAVWSPPGRSGFPNGAAVRSAPTMLRGFGPAG
                     IRRTLRLVARMEKAHPPEPHWYLELLATDAHLRGRGVGSRLIGPGLDRADAEGVGAYL
                     ESSKWDNVPFYRRHGFEVVEEMVAVPGSPPMWRMWRDPR"
     gene            complement(14856..15560)
                     /locus_tag="PLZ72_07825"
     CDS             complement(14856..15560)
                     /locus_tag="PLZ72_07825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA36239.1"
                     /translation="MTDPGPTSSAAPDEGHRQYRYSPPPGSTTILLVRHGESAPERSD
                     RPFPVRDGHGDPELAPDGREQARRVGERLAAEHRAGATIDAVWVTTLQRTQQTAAPLV
                     NRLGLTPNVVADLREVHLGEWELQFRAKVASGDPIALRMFTEERWDVIPGAEPGDEFA
                     ARLRRGIDTIVAAHPDQRVVAVVHGGVIGQLLATATGSSPFAFIGADNGSISELVVQS
                     DGRWRLRRYNDVAHLR"
     gene            complement(15574..>16015)
                     /locus_tag="PLZ72_07830"
     CDS             complement(15574..>16015)
                     /locus_tag="PLZ72_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36240.1"
                     /translation="VMDPSVQEAADHIDDVTAEMCGLDLSELDEPGTTDDTLPDDTLP
                     DDTLPEPGAAAGDIDLEHIDRIKDEHQGSSWVEKLSTTSIMNDTAVSLSADASDPITP
                     AEAMEACQVVRSALVSINPDVTVSITNGETAVVQAPAGGACAPA"
BASE COUNT         2308 a         5913 c         5551 g         2143 t
ORIGIN      
        1 ccgccgttgt cgtcgggcgc cgcgctcgac gccgatgccg gcgccgcggc ggagccgtgg
       61 gcgtgaccgc catcagcagc agctcccccg gccgacaccg cgttccgctt gaccatccag
      121 gcccgccagt cccggtagtc ctcgtcggtg gcgccggcca gggcgacgac cagcgcggcg
      181 gcgtcgagct cggcgccgga gtcggagcgg gccgtcgccg agaccaggcg gtcgaggccg
      241 atcgaacccc gcccctgctg catgtccggg gaccccgacg tcggagccgg gggcgccatg
      301 gccatggtcc cgccgccgta ggtgtcgacg ccgagcgccg aggcctcggt ccagtagctc
      361 ttcgggttga agcccttgtc gcagcgctcg tcgtcgatgg ctgccatctg ggtgcggtgc
      421 tccgccgcgt cggcagccgt gccctcggtc ccgtggctgt ggcccgccgc cccttcgccg
      481 tccgatccgg tcgagacgag tccgacggtg accagtccga gcaccgcgac cgccgcgatc
      541 gacgggacga gcacacccac ccggagccgt cgcggcgccc acgccatcac cacggccacg
      601 acgaccgccg cggcctcgag ggcggcgcac acgccgtcga tcagcccgac ctgctcgacc
      661 actcccgcgt gcgagccgaa cggcaggccc accgttcggg cccagatcca cacggccagc
      721 gcggcggcgt tgagcgcggc ggtggccacc gcgacgccgc gggtcccacg gcgcagcagg
      781 aagacgaccg ccagcgcgat ctggagccag cccacgaccg cgaagccgac cgggtcgatc
      841 caggacccgc cccccgcgtg cgccgggacc atgaccaggt ggatgacgcc cgcgccaccg
      901 gtgagcccgg cgagcaggat tcccaggtcg gaggccccgt cgctccaggt ctccgaggcg
      961 ctgggcgcgc ccggagaacg gtggtcggag cggttgcgac ccgctgggtc catggttcac
     1021 accccccggc ggacacccgt gcggcccgcc cgtcgtgatg acgtctcgcg tgacatcggc
     1081 cacccactgt agggcccgac agacgaatgg aggtcggcga cgcccgtccg gtagagtcgc
     1141 acccctatgt cgaagcgcac ccagaagaag aaggcgaacg tgcgtcgcaa gaaggccaac
     1201 cacgggcgca agcccaacat ggggcgcaac tcctgacgat cgccgcggcc ggatgggacc
     1261 accacccgtg acggatcggc cgcaccagct cacccgactg accctcggcg acgccgccga
     1321 cgcgtggcgt cgcgccggtt tcgccgtcga cgcgaacggc agtttccgac tcggccgcac
     1381 cgacgtggtc gccctgggca caggtcgacc gggcgtccgc ggctgggcgc tggacggcat
     1441 cgaccacccc ctcgacgggc tcgccaccgt cgacggccac gacgacgacg ctccggaccg
     1501 ctccccgtcc tcgcatccca acgggatcac ccgcctcgac cacgtcgtgg tgagcaccgg
     1561 cgactgcgag cgcacgaccg ccgcgttcag ctccgccggc ctcgatctcc ggggcacgcg
     1621 gagcacgacc gcattcgggt cccccgtccg acagagcttc ttctgggccg gagacgtgat
     1681 cctggagctg gtcggccccg acgacgggga gccgaccacc gacgagccca cgtcgatcct
     1741 cgggctcgcg atggtcgccg acgacctcga cgccaccgcc gagtggctgg gcgaccgggt
     1801 cggcacgccg aaggacgccg tgcagcccgg caggcgcatc gccacgctcc gccaccgcga
     1861 cctggggctc ggtctgccgg tcgcggtgat gacaccgcac cgccgctgag ctccgctgcc
     1921 cggcccgatc cgccaccgga ccgcccgcga tggtgggcga cggttaccgc tcggtaacat
     1981 ggcggcatgg ccgactaccg agcacccgtc aaggacgccc tgttcaccct ccgtcacatc
     2041 ggcatgatgg aggacctgtc caagaccgag cgctacgccc acgccgatcc cgagaccgtc
     2101 gccgccgtgc tcgaggagca gggccggttc atgcaggagg tgttcgcccc ggccaacgcc
     2161 atcggcgacc gggtcgggct caagtggtcc ccggacgggg tggagacgcc cgcggagttc
     2221 cgcgccgcct acgccaagtt cgtcgaggcc ggatggcagg gcctcaacgc cgaccccgag
     2281 tacggcggag ccggcttccc cgaatcggtg tacgcctgcg cgtccgagtt cgagaccgcc
     2341 gccaacggcg cgctcaccat ggcccccggc ctcaccaccg gagcgatcga gtgccttcag
     2401 cactggggca ccgaggagca gaaggaggtg tacctccgca agctgatcac cggcgagtgg
     2461 accggcacga tgaacctgac cgagccgcag gccggatccg acgtcgggct gtgcaccacc
     2521 aaggccgtcc cgcagggcga cggcacctac ctgatcaccg gcaccaagat cttcatctcg
     2581 ttcggcgagc acgacatggc cgacaacatc gtgcacctgg tgctggcccg cctgcccgag
     2641 gctccccccg gcaccaaggg gatctcgctg ttcatcgtcc ccaagttcat ggtgggcgac
     2701 gacggctccc tcggcgagcg caacgacgtg cggtgcgtgt cgatcgagca caagatgggc
     2761 atccacgcgt cgccgacctg cgtgatgagc tacggcgacg acggcggggc ggtcggctac
     2821 ctggtcggcg aggagaacca gggcatgcgg gccatgttca cgatgatgaa ctcggcacgc
     2881 atcgcggtcg gcatccaggg cgcggcgctc ggcgacgccg cctaccagaa ggcgctggcc
     2941 tactcccagg aacggcgcca gggccgcgag atcggcggcg actccaagga gccggcgttc
     3001 atcatcgccc accccgacgt gcggcggatg ctgctgttca tgcgcagcca cgtcgaggcg
     3061 atgcgggcgc tcatcgtcta caacgccgcc gcgctcgacc tgtcccgcgc cctcgacggc
     3121 gacgaggcga ccgcctgggg cgagatcgcc gagctgctca ccccggtcac caaggcgtgg
     3181 tgcaccgacg tcggcatgga ggtcacgtcg accgcggtcc aggtgttcgg cggcatgggt
     3241 tacatcgagg agtccggcgt cgcccagcac ttccgcgaca tgcgcatcgc ccccatctat
     3301 gagggcacca acgggatcca ggccatggac ctggtcggcc gcaagctggg cctgcggatg
     3361 ggcggcgtcg tcaacgacct gttggaccgg atgaccgcga ccgtcgccga gctcgacgcc
     3421 gccggcgagt cgttcgcatc gatggccacc gagctgcggg ccggcatcga cacgctgtcg
     3481 gacgtgacga gctggatcat gaccagcggc ctggccgacc ccaaggaggc gctggcgggc
     3541 gcgacgccct acctgcgcat cttcgggatc gtcaccggcg gctggctgat ggcccgtcag
     3601 gccctggcgg cccgggcgga gctcgacgcc ggttcgtccg actcggccta cctcgaggcc
     3661 aagatcgtga ccgcccgctt ctacgccgaa cagcagctgc cccaggccgc cggttgggtc
     3721 gcctcggtca agggcggggc cgcggtcctc tacgacgtcg acgccgacca gctcgcctcg
     3781 gtgtgacggc gggcgcgtcg agcggtccgc cacggcgccg gtagccttcg ccgcgcatgc
     3841 tcgaccacgt cttcaccgat gcgatcgggg cgctgcgcga cgccctggag aaggcgttgc
     3901 tcgaacgtca ggcgttcgag gagcggttcc agtccgacgt gctgttgggc gacctcacgt
     3961 gggagacctc ctacgggcta cccggtgagg ggttgccgcc gcgggtgcgt gcggacctga
     4021 ccctcgagtg gccgacgtgg tcgcaggccg cctaccgctc ctggtacatc gaagaggagc
     4081 tgcccgagct gccccgcatc gacatcgagg tcgtgctgcg catccagcgc ctggtgtcac
     4141 cgcccgatcc gcgcaaggtg ctcgacgcgc tgcccctcga gagcccggcg atcggcggcg
     4201 agcgactgca ccggtccggt ccgacggtcg aggcggtctc cgacgaggac ctgaccgaga
     4261 ccgagcacgc gatcgagatc agctacgagg gcagctacga gctcgacgag gccaccctgg
     4321 ccgacgggtc caccctcgat gaccacttca gcgccatggg cgggtggatc tcctccaccc
     4381 tggttcgcct cggcgacctc aacttcgagt tcctcccccc cctcgaaaag gacgactcct
     4441 gatatagggg tcctcaacga catcccttgt cacaatcccg accacagaaa ccccgacacg
     4501 aaatccggag gccgccgccg tgaccgagtc cgacgtgctg caagtcgagc gatccgacgg
     4561 gtacgccgtc gtcacgctcg accgagcgaa cgcccgcaac gcgctgaacg ccacgctcct
     4621 ggccgggctc acctcgacca tggccgagct ggactcggac ggatcggtgc gtgccatcgt
     4681 gctgaccggg gcggacccgg cgttctgcgc cggcctcgac ctcaaggagc tcgccgcgtc
     4741 acccgaacgg ctcgccggcg ggttcggcga cagatccgag ctgcgccggc cgttcccgga
     4801 catgtccacc ccggtcatcg gtgcggtcaa cggtgtggcc atcaccggcg ggttcgagct
     4861 cgcgctggcc tgtgacttcc tgatcgcctc ggagcacgcc cgtttcgccg acacccacgc
     4921 gcgggtcggc atcatgcccg gctggggact gaccgtgctg ctcccccagg cggtcgggct
     4981 gcgccgagcc cgcgagctgt cggccaccgg caacttcctc gacgccgaaa ccgcctacga
     5041 ctggggcctg gtcaaccacg tcgtggccca tgaggacctc ctgccggtgg cccggaagct
     5101 ggccgccgac atcgccggca acgaccccga cggagtccgt cagatcctgt ccacctacac
     5161 ggccggatcg ctcctcaccg ccgccgacgc cctcgatctg gagcagcgcc acagccggga
     5221 gtggctcgcc cgacgagacg ccggcgacct cgacgagcgt cgacgggcgg tcaccgaacg
     5281 cggtcgcacc cagctctgag cggcctccgc cgatttctga ggtcgggatt gcgacaaggg
     5341 atgtcgatga ggacccctat atctgggggc ggggggggat gggttcgaac tcggcggggc
     5401 gcttgtcggc cttggcgccg aactcctcca acatgtcgcc gggctggaac atgccggcct
     5461 gccagccggc catgtagcgc aggctgtcgg ccacggagtg gtcccgggtg tagttgacca
     5521 tctccttggt gccccagatg gcgagcggcg acttggacgc gatccgggcc gcgatctcga
     5581 gcgtgccgtc gaccagggac tggtggtcct cgaagacctg gttcaccagc ccgcactcgt
     5641 aggcccgctg agcgggcatc cggtcaccgg tgtaggcgag ctcgcgggcg acgccctcgg
     5701 ggatgatctt gggcaggcgc tgcagcgttc ccacgtccgc ggtcatcccg atgttgatct
     5761 cctgcacgca gaagaaggcg tccgccgagc agtagcgcat gtcggccgcg gtcaccatgt
     5821 cgaccgcgcc gccgatgcag ccaccctgga tcgcggccag caccggcatc cgcgccgtct
     5881 cgagggcggt gaaggagtcc tgcaggtgct ggaccaggtt ccacaggtgg gcccgacgac
     5941 ggccctcctc gctcacgccc ggcgagccgg cgagcccgcc cccaccggtg aacaccgaca
     6001 ggtccatccc ggcgcagaag tgcttgccgg tcgaggagat gacgatggcc cgggcggagc
     6061 cggcggcgtc gatctcgccc acgatctgcg ggagctcgga ccagaagctc gggatcatcg
     6121 agttgtaggc gtccggccgt gacagctgga ggtgggccac gtgatcggag atggagacgt
     6181 cgaagcactc gtgcacgacc gcgaccgtac cgcccaccct ccggtcgtgc ccgaagccgt
     6241 cgcgtcgtgc tcggtagcct gcggcggtgc cgagcgacga cccgtaccgc ctcccccgcc
     6301 acgtcctgcc ccaccgctac gacctggagc tggtcgtcga ccccgaacag cccggattct
     6361 cggggcgggt ccgcatcgag gtcgaggtga tcgagacgac cgaccggatc accctcannn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg gtcacccagt tcgagtccac
     6541 ccacgcccgc cgagcgttcc cctgcttcga cgagcccgac atgaaggccg tgttcgcggt
     6601 gtcgctcgtc gtccccgacc acctgctggc ggcatcgaac gcgtccgagg tcgaccgcac
     6661 cgacctcgga gacggaaccg tccgcgtcac cttcgccgac acgatggtga tgtcgacgta
     6721 cctggtcgcc ctcgtcgtcg gccccctcga ggtgaccgac acccggatga tcgacggccg
     6781 ggatcgaccg atcccactgc gggtcatcca cccggcgggg tccgacggcc tcaccccgtt
     6841 cgcgctcgag gtcgccgatg cgggcatccg gctcctcgag gactactacg acatcgcgta
     6901 tccgggcgac aagatcgacc tggtggccgt ccccgacttc gccttcgggg cgatggagaa
     6961 cctggggctg atcaccttcc gcgagatcgc cctcctggtc gaccccgacg aggccaccca
     7021 gcccgagctg caacgtgtcg cggatgtcat caaccacgag ctggcccaca tgtggttcgg
     7081 cgatctcgtg accatgcggt ggtgggaggg gatctggctc aacgaggcct tcgccacctt
     7141 catggaggtc gccgcctccg acgccttccg acccgagtgg gacacctgga ccggcttcgg
     7201 actggcccgg gccgccgcgt tcgacaccga cgccctcgcc tcgacccgac cgatcgagtt
     7261 cccggtccac agcccggccg acgcggaagc catgttcgac gtcctcacct acgagaaggg
     7321 agcatcggtg ctgcgcatgc tcgagcgcta cctgggtgcc gagcgcttcc gggacgggat
     7381 ccggcactac ctccgcaccc acgcctacgg caacaccgcc accaccgacc tctgggacgc
     7441 gctcgaggag gtgaccgggg aaccggtgcg gcggatcatg gacgcctgga tcctctcggg
     7501 aggccacccg gtggtgaccg cccggctcgt cgacgacggc atcgagctcg accagaccgc
     7561 cgccgcccgc tccggggatc cgtcgctggg cgaacgctgg ccggtgccgc tcgtggtcac
     7621 cgccaccgtc gacggctcca cccgccacga gcgcgtcctg ctcgagggac ccaccgtcgt
     7681 cgacctcggc ggcgcaccga tcgagctgcg actcaacacc ggcggagacg ggttcttccg
     7741 ttcggagctg cccgcgtcgc tgcgaaccgc cgacgcggtc gaccccgact ccacaccgct
     7801 cgagcggttc gtgctcgtcg acgacacgtg ggcggcgctg ctgcgcggca ccctcaccgc
     7861 ggcggaggtc gagcagctgc tccgcctcgt cgcgtggcgc gagaccgacc cgtcggtgtg
     7921 gcgccggatc gcccaggccg tccgcgagct ccgtcggctc gaagggtggg acggcgccgt
     7981 ccgggcgccc cgcctggcgc aggacgtcgc cgccctcccc cgttcccgtg ccaccgaggt
     8041 gatctccgga gtcgacgagt cgggtcggga gagatggcgg gacctgaggg gcatcctcac
     8101 cgtcgtgctc ggcgtcgccg ggaacgacgg tgacatccgg gatctggcgc gggacatcgt
     8161 gcacaccgac cccgaacgac tccgggagcg ccgcgacgtg gacccggctc tcggcgccgc
     8221 ggcgctcgac gtcgtcgccg ccaccggcga tcgggccgac cacgacctga tcgaacagcg
     8281 gtggcggacc gcggccaacc cccaggacac gatccgccac ctctacgccc tggccgacac
     8341 gccggtcccc gagtgcttcg agaagacgct ggatctggcc ctcggcgagg tgcgggccca
     8401 ggacagcggc tacctgctgc ggcgggcgtt ggccaacccc cgccacgcgg gtcgcgccgt
     8461 cgagttcgtc tcacgccgct gggacgacgt ccaggccaag gtcccctcca gcgccctggt
     8521 ccgcatgctc gagggtgtcc gcagcatcac cgacgcggac ctcgcggccg acgtcgtcgc
     8581 gttcgccgag gcgcacccgc tgccgagcgg ctcacgggtg ctggcccagc acctcgagcg
     8641 gatggccgtc aacgtgacgg cggcacagcg tctgcgctcg ggttgatgcc gctctcgggt
     8701 cccgacccgg catcctgaac cgaccatgtc cggacctccc gcgcccggcg gccaccgtgg
     8761 ccgcaccgtc gagcacatcg cccgcccccg gaccgcgggg atcggcctgg atgtgttcgc
     8821 ggtcgaggac cgctcggccc aggccgtgtg ctcgtcgctg gagccgggcc ggttcaccgt
     8881 gtcggtcgac ggtccccgcc ggcgtcgacc gctcacggtc gagcacccgg ggggaccggt
     8941 ggcgtgggag ctggccgggc tcacccccgg gcgccgacac cgggtgacgg tccgcaccgc
     9001 ggacggacgg acggtcgccc ggcgggcgtt ccgcacgctg cggccgccgc cgggcgagga
     9061 gctgttccgc ttcgccaccc tcaacgacct gcacctgggc agagaggaca agcgcgggac
     9121 ggatcgactc ggcacgcgac ccggagcgcg gacgccccag gacatggcgc gcgacgccct
     9181 gggcgacgcg ctcgcctggg gcgcctcgac gatcgtggtc aagggcgacg tgtgcgacca
     9241 gtcgttcgac cggtactggg accaggcgct ctcgttgttc gccgcggtcc cggtcccggt
     9301 gtggatgctg ccgggcaacc acgacaccgg cacccggcgg gcggtcgaac ccgacgtggc
     9361 ggcgcgcctc cggggcctgc ggatgatccg cggcgtggag cacctcgacg tcgacggcct
     9421 gcgcatcgtg ctggtcgagt cgggcatccc cgacggcggc tggggtgaca tcgcccgcca
     9481 tcgacacgac gtcgccgacc tggtggccga cgcccgggcc gcgggcaccg gcacgttcat
     9541 cggcacccac caccagccgc agcggttccg gacgccgctg tactggccgc acggaacccc
     9601 cggtccgaac gccggggagt tcgcccggac cgtccgggcc gcggacgccg gggtcatggt
     9661 cagctccggt cacacccatc gctgtcgacg gcgcgacgtg gccggggtcc cctggaccga
     9721 ggtcgcctcg acgcaccact tcccggccac ctgggccggc taccgggtct tcgagggcgg
     9781 cctcatgcag gtggtccgtc gcaccgcggt tccgggcgcg ctggcctgga gcgagaccac
     9841 ccgcgacgcc ctgggcggca tctgggcgct gtgggccacc gggacgctgt cggaccgcag
     9901 cttcgcgctc gactggcgcc ggctcaggtg atctggcccc gggcgtcgag gaacgcggtg
     9961 gcgaccccca cgtgcaccgc gacgccggtg gccatgccgt cctcgttgag gaccatccgg
    10021 ttggagtggc acgacggggc ctcgaacggg ttggggttct cgggtgggca gacccccagg
    10081 aagaacatgg cgccgggcac ctcgcgcagc acgtaggaga agtcctcggc ccccatgacc
    10141 ggcgacggca tctccacgac gtggtccgcc cccaccacct cggcggccac accggaggcc
    10201 caacgtgtga acgcggcgtc gttcaccgtc accggatagc cctcgacgat ctccaccgtg
    10261 gccgtgcacc cgtgggcggc ggccacgccc tgggcgacct ggtcgatgct cgcccacacg
    10321 gcatgtcggg tgtgctcgct gaccgcccgc agcgtgccgg tcagagcggc ggactcgggg
    10381 atcacgttgt cggtcgtgcc ggcgtcgagc tgcgcgatgg tcagcacggc cgggtcgaac
    10441 gcgttgaccg tgcgggtgat catcgactgc agcgccagga cgatctcgca cgccaccggc
    10501 accgggtcgg tggcccagtg cggcgacgac gcgtggccgc cccggccggt cacgaccacc
    10561 ttcaccacgt cggccgaggc cagagccgca cccgagcggt ggatgacgaa gtcggagaac
    10621 atgttgggag cgacgtgcag ggcgaacgcg gcgtcgacgc cgtcgaggac gccttcgtcg
    10681 atcatgtcgg tcgccccgcc gaagccctcc tcaccgggtt ggaacatgaa ccggacccgg
    10741 cccgccagcc gatcgcgccg gccggccagc acggtcgcag ccccgaccag catcgcggtg
    10801 tgggcgtcgt ggccgcaggc gtgcatcgct ccgtcgacga ccgacgcgaa gtccagaccc
    10861 gtgtcctcgg gcatgggcag ggcgtccatg tcgccgcgca gcaggatcgt cgggccgtcg
    10921 ccggcgccgc cgacgagatc cgcgaccacc gagctcagcg cggtgccggt gctgatctcg
    10981 aggccgaggc cgtccagcgc ctcgaggacc ctgccctggg tgcgcggcag gtggttcccc
    11041 agctccgggt gcacgtggag gtcccggcgg agctccacca cgtcggggag caccccctgg
    11101 gcggcggcca gcaggtcgtc ggtgatcatc ggtcatcctc cacggccccg accacccccc
    11161 ggtgggatcg gcccgggcgg gggcgacggg gcggtcacac cagggcgagc accagaccga
    11221 tctggcccag atggtacgtc gtcatcacga ccagccggga ctgcgggaag tcccgcacga
    11281 accgggtcag cccgatcacc ccgtcggaca cgtagaacaa cgccgctccg aggaccgcga
    11341 tccagatgcc ggtgccgacc gcgctggcga ccatcagcga gatcaccgtg atgtaggcgg
    11401 ccaccggcac ggcgagccgg gggtcctgac ggcgcgcccc tgagacgatc ggcgctccga
    11461 tcaccgccat gaggaccgcc acgaccacga cgcccaccgc gaagcgcggg aggtcgacgc
    11521 ccaggttcca caacccgacg acgtaggcga cgtggccgac caggaacgac gccagaccct
    11581 cgacgaagtg gtcctcgagc atcaggaaga catccccggc gagggagcac agcaggcccg
    11641 ccaccatcca gcccctcgcc gcgcccgacg acaccgggtc cgcggccagg gcggcgccga
    11701 tcagcaccac catggtcaac ggcttgagga cgtactcggc gcggcgtcgg tcggtgccga
    11761 ccgcgatcca gtcggccacg gcgacgacga gcgtgagccc gatcaggaca tcgaacgtgg
    11821 tcgtcatcgg aacagctccg cgaccggctc ccacgcgagc gactcccgca gcctcatccg
    11881 ggcctgcatg accagacggg gccggttcac ccccagcacg ccggtgcagc gctccccgcg
    11941 tcggaaggcg acgacgaacc ggccgtcgtc gatcgtgccg tccaccacgt gcaactcgtc
    12001 gtctccgtcc ggtcgtccgg ccatctggat cttggcatcg tactggtcgg accagaacca
    12061 cggcaccgac gcgaaggcca ccgcggccgg atcgcctccg agcagccggg tcgcggccgc
    12121 ctcgccgtgc tcgatcgcgt gctcccagtg ctcgacgcgc atccgctcgc cgaagtgctc
    12181 gttgaaccag ctggccacgt cgcccgcggc ggtgatgccg ggggcggcca ggccggtgga
    12241 gtcgcagacg atcccgtcgg acaggtcgag tcccgagccg ccgagccatc cggtgctggg
    12301 ggtgacgccg atgccgacga ccaccacctc ggcgtcgacc gacgagccgt cggcgaggcg
    12361 gaccgcccgg acccgtcccg aatcatcggc gtcgaggcgg tccacgcccg cccccaaccg
    12421 cacgtccacg ccgtggcgtc ggtgcaggtc ggccacgaac gatccgatcg gggcgggcag
    12481 ggcccgcggc aggggcaccg gggccgcctc gatcatcgtg acctcgtgac cccgaccccg
    12541 acacgtggcc gccacctcgg cgccgatgaa gccggctccg atcaccgcca cccgcgccgg
    12601 accggcgtcg agctcccggc gcagggccaa cgaatcggcc atgtcgcgca ggacgtgcac
    12661 cccgccgggg ggcgcaccgg ggagccggcg tggggtggca ccggtggcga tcacgatgcc
    12721 gtcggcggcc tgctcggtgc cgtccgacag cgtcacggtc cgggtggcga cgtcgagggc
    12781 gaccgcgtgc acgcccaacc ggaggtcgag atcgaggtcg ggaacctcgg gcgcggaagg
    12841 ctgctgcgcc cggtcgggct cccactcgcc ggcgaggacc tgcttggaca gcgggggccg
    12901 gtccgagggc agttccgccg acgggtcgac gacgacgatc gtggcggtcg ggtcgccgcg
    12961 gcgcagggtc cgcgccgcgc tcaggccggc cagggacgcg ccgacgacga ggtaggtggt
    13021 catcatcggg gatcggggat cacggacggg acgggtcgtt cgggatcggg atgcccgggg
    13081 ctcgaccccg gacgccgagg gtcagccctc gacggtgatc gcctgcttgg ggcaacgccg
    13141 ggcggcttcg gtcaccttct cacggagctc ttcggacggc tcctcctgga ggacgtagag
    13201 gaggtcgtcc tccctgacct cgaacacctc gggtgcgatg cccatgcaca cggcgttcga
    13261 ctcgcacagg tcgtagtcca cgacaacctt catgcccatc ccctttgctc ctctccgcac
    13321 cggccggcga ccgagcgaac gttcaggagc actctaccga cgggggtgcc gtgtcagaag
    13381 atcgagtagc ccccgtcgat caccaggagg tcgccggtgt ggaacgtcag ggtcggatcg
    13441 gccaggaacg cggccgcctc ggcgaagtcc gacggcacgc cccaacgccg caccggggtc
    13501 cgggccgtgg tgttcgccat gaacttctcc caccccttca gcggcgcggt catgtcggtc
    13561 tcgacccagc ccggcatgat cccgttgacc cgcacgccgt gacgggcgag ctcgaccgcc
    13621 agtccccgta tcagggcgat catcgccgtc ttcgagcacg agtacgcctg gttcgccggc
    13681 gccccgtgga tggccgaggt cgacgagacg ccgatgagcg aaccgccttc gccgcgctcg
    13741 aggaggtggc gggtggcggc ccggaagcac aggaaggctc cgtcgaggtt gaccgccatg
    13801 acccgacgcc actgctcgag ggtctgatcg acgaacggcg caccgccgcc gatgccggcg
    13861 ttggcgatca tcgtgtcgac ccgtccgtgc accgcgaggg tctgcgccat ggcggcgtcg
    13921 acggcggcct cgtcggcgac gtcgacgacc accgagctcg cgaccgcacc cgcggcacgc
    13981 agcgcctcga cagcggcgtc gttcttggcc tcgtcgcgac cccacaccgc gaccgcggcg
    14041 cccgcggcgg ccacgccgtg ggccatcccc aacccgatgc cgccgttgcc gccggtgatg
    14101 accaccaccc gtccgtccag gtccgtcacg ccgttcgcct cccgtgctcg tacgcggtcc
    14161 ggagcggatc cggcgcgtcg tgcggcacgg tacccgccgc ggccggatcg gccggtagcg
    14221 tggccgcccg tgtcgggatc ccccttgcgc atcgccaccg cccgggacgt cgacgccctg
    14281 tcgcaggtcc tggcccgcgg cttcggcgac gacccgatct ggcggtggat ggccccgacc
    14341 ggcacccggt ggccggatcg cctcggtccg atcttccgac acctgctccg accgccgatc
    14401 gggttcgggg cgacgtggac caccgatgac ctggagggca ccgcggtgtg gtccccgccg
    14461 ggtcggtcgg gtttccccaa cggcgccgcg gtccgctccg ccccgaccat gctgcggggg
    14521 ttcggtccgg cagggatccg acggacgctg cgtctggtgg cccggatgga gaaggcccat
    14581 ccgcccgagc cgcactggta cctggagctc ctcgcgaccg atgcccacct gcgggggcgt
    14641 ggggtgggca gccgtctgat cggaccgggt ctggaccgcg ccgacgccga gggtgtcggc
    14701 gcctacctcg agagctccaa gtgggacaac gtccccttct accgacgcca cggcttcgag
    14761 gtggtcgagg agatggtcgc cgtgcccggg tcaccgccga tgtggcggat gtggcgcgac
    14821 ccccgctgag gccactcccc ccgccggtca ggccctcatc gcaggtgggc cacgtcgttg
    14881 tagcggcgga gccgccagcg accgtcgctc tggacgacga gctcgctgat cgagccgttg
    14941 tcggcgccga tgaacgcgaa cggggagctg cccgtggcgg tggccagcag ctggccgatc
    15001 acgccgccgt ggaccacggc gacgacccgt tggtccggat gcgccgcgac gatcgtgtcg
    15061 atcccccggc gcaaccgggc cgcgaactcg tcgcccggct ccgcacccgg gatcacgtcc
    15121 catcgctcct cggtgaacat gcgcagcgcg atcgggtcac ccgaggcgac cttggcccgg
    15181 aactgcagct cccactcccc gaggtggacc tcgcggaggt cggccacgac gttgggggtg
    15241 agcccgagcc gattgacgag cggcgccgcg gtctgctggg ttcgctggag ggtggtcacc
    15301 cacacggcgt cgatcgtggc accggcacgg tgctccgcgg cgaggcgctc acccacccga
    15361 cgggcctgct cacggccgtc cggtgccagc tccgggtcac cgtggccgtc gcgcacgggg
    15421 aacggccgat cggagcgctc gggagccgac tcgccgtggc ggaccagcag gattgtggtc
    15481 gacccgggag ggggtgagta gcggtattgg cggtggccct cgtcgggcgc ggcggaagag
    15541 gtcggtccgg ggtcggtcac acccgacagg atgtcacgcc ggggcgcagg cccctccggc
    15601 gggcgcctgc accaccgccg tctcgccgtt ggtgatggag acggtgacgt cggggttgat
    15661 cgacaccagg gcggagcgca cgacctggca cgcttccatg gcctcggcgg gggtgatcgg
    15721 gtccgaggcg tcggccgaca acgacaccgc ggtgtcgttc atgatcgacg tggtcgacag
    15781 cttctccacc cacgacgacc cctggtgctc gtccttgatc cggtcgatgt gctccaggtc
    15841 gatgtcaccg gccgccgccc cgggctcggg gagcgtgtcg tcggggagcg tgtcgtcggg
    15901 gagcgtgtcg tcggtggtcc cgggctcgtc gagctcggac aggtcgagcc cgcacatctc
    15961 ggcggtgacg tcgtcgatgt gatcggccgc ctcctgcacc gacgggtcca tgacc
//
LOCUS       DAOQOD010000062        15963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1299_length_15963_cov_11.319965, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000062 DAOQOD010000000
VERSION     DAOQOD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1299_length_15963_cov_11.319965"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1004
                     /locus_tag="PLZ72_07835"
     CDS             3..1004
                     /locus_tag="PLZ72_07835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA36241.1"
                     /translation="MADHTPPGGARWGMTIPFDGVPLHEQRDWYRELEDLGYTDVWSA
                     EAMGADAFTPLALASAWAPSLRLGTAIVPAFTRGPAVLAQSVASLADAAPGRFAFGIG
                     TSSDVIVGRWNGIPFEEPYRQVRDMVRFVREALAGEKVSGTFGRSEVKGFRLGVRPEV
                     DPPILVAALREGMLRLAGREGDGAIINWLSAEDVSTVAPIVQQAAADAGRGPKEVVAR
                     IFVAPTTDRDLVMKVGRYAIAAYLTVPVYAAFHEWMGRGEQLAEMSRRWADGDRAGAL
                     DAIPDSLVDELIVWGSPEQCRDHIGRYVANGVTTPAIAVLPFGVDQRQAIRDLAPVG"
     gene            1009..1980
                     /locus_tag="PLZ72_07840"
     CDS             1009..1980
                     /locus_tag="PLZ72_07840"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNA36242.1"
                     /translation="MTDQTAGDPPAPVTIVDVAPRDGLQSDGLIVSTEAKLELIERIV
                     RAGIRRVETTSFVNPARVPQMADAAEVMAGLAARRAASPAGDPLREASFIGLVLNRRG
                     LDRALEAGCDEVNAVVVVSDTFSERNQGTTTDEGIVVWAEIAEAAAEAGVPVSVTLAA
                     SFGCPYEGEVPVDRLADVVSRCVEVGGVEVALADSIGVAVPTDVRARLAVARDAIAAA
                     GTGQHLRVHFHNTRNTGLANVATAVEEGVTVVDSSLGGIGGCPFAPGATGNVPTEDVA
                     YLLSRMGHDTGIDLDELVGAVHWMQSDALLDRDTPGALARAGVFPRR"
     gene            complement(2148..3743)
                     /locus_tag="PLZ72_07845"
     CDS             complement(2148..3743)
                     /locus_tag="PLZ72_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA36243.1"
                     /translation="MLLHQVIEFAAGTSPDVDALVAGDRRWTFSELERDVARMGTTLA
                     HVVARDERIAWFSDNRPELVMALYAVPRAGALLVLGNIRHTAAEQAAMLKASSASVVI
                     GSAEQLARLTEHADELHDVRRWYCLDGVATHDGPVTDDGAELPSGTRDISELFAREPE
                     PPRSGLDDDTHVWLIHTSGTTGRAKGALLTHRSLVAAATNTVLARPISDDDVYLFPFP
                     LFHVAAYNVVVHHLGRRPVVLLPRFDPDEVLTAIRDESVTTVSLAPTMLAMLLEHPER
                     SVEDLSSLRRIGYGASAMPLDLLRRTLSELPEVGLAQGYGMTELSGNAVFLGPDEHRR
                     AGADEPWLLSAAGRPAPLVQIRIADDRGQEVPAGDVGEVLVRGDQVCAGYWDDPGATV
                     ESHHGEWFRTGDLGRLDEAGYLYIVDRKKDIIISGGENVSSREVEDIVSEHPDVAAVA
                     VVGLPDDTWGERVCAVVVWNGTDDPTRATDLDARARALVDWTEGRMAGFKRPRVIVSA
                     PELPLNASGKVDKHQIRDHLRPS"
     gene            complement(3802..4869)
                     /locus_tag="PLZ72_07850"
     CDS             complement(3802..4869)
                     /locus_tag="PLZ72_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36244.1"
                     /translation="MKVRLEPADEYMHPGPEPTFNESMYFNLYDPDARLGGFVRIGNR
                     PNEGHAEMTVCLYLPDGRVGFMFRRADIDSNDRFDAGGATIDVVEPFRTDVEHAEGAG
                     GGRIDVGYHGQVVLLEQPLDMAHPRRAFTENPYADARVQLTYEGISRPFGGEPEERHE
                     RPGEEFAKGHYEQLVRGTGTIAVGDSTWSVAGLGLRDHSWGPRTWQAPWYYRWLTMNF
                     SDGTGLMLSRIARPDSRGTRSGFVWDGRELHLVGDVTLRTEWVGDDSHHSRIHATFST
                     VRLGTDGITPGIERTVTGDVLNLIPLRNRRDERVTRISEGLTRWELDRALGDDPTATV
                     GHGLSEYLDQIVDGNPVGLDE"
     gene            complement(4911..5426)
                     /locus_tag="PLZ72_07855"
     CDS             complement(4911..5426)
                     /locus_tag="PLZ72_07855"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HNA36245.1"
                     /translation="MTLTLRREHTLADTVSPGMRLLLVGLNPSPYAAEVGVGFARPGN
                     RAWPALAAAGLAEVDRDPLHLLHHAGIGMTDLVKRTTATAGELRAEEFRDGLERLEVL
                     CDWLQPTAVCVLGITGWRAATGMRSARLGPQAATLGGCPVYVMPNPSGLNAHTDVDDL
                     VRHLRAATELR"
     gene            complement(5645..6460)
                     /locus_tag="PLZ72_07860"
     CDS             complement(5645..6460)
                     /locus_tag="PLZ72_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36246.1"
                     /translation="MELRDRVIVVTGGGSGIGAAMCRRFAVEAPTAIVVVDINGDAAA
                     SVADDVREAAPGTEVTSRAIDVADEAQVGAMIAEVFLHHGRLDLFCANAGIGTGMGVE
                     APDPAWEQIWHVNVMAHVYAARALLPAWLEAGEGHLLITASAAGLLTNLGDAPYTATK
                     HAAVGLAEWLSVTYGDRGLHVACLCPQGVRTPLLFGDSDSPTDGALAADVVKAQRILE
                     PEEVADTVVEGLADERFLILPHPEVADYERARATDRDRWLGAMRRLQAKLLGR"
     gene            complement(6508..7536)
                     /locus_tag="PLZ72_07865"
     CDS             complement(6508..7536)
                     /locus_tag="PLZ72_07865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA36247.1"
                     /translation="MSTDAASIAPDRPVGLTFASFQALGLDVGLAMARTARDAGCSSF
                     WTAETVGTEAFATLGAVAAGVGGLDLGTGVLALQLRTPQVAAMGAATLQALAPDREVL
                     LGVGISSPVVVGRWHGAEYGTRPVAQVREFLELTAACLSGEPVTYDGEFYSANRFRLG
                     VRLHGPRPQLVLGALNQRMLRLAGELADGVLLNYLPASAVPWCVEQVRAGEQFAQREP
                     GSCRIHAYVHVGVCDPDRARPSGRKDLFSYAVVDAYARAFERAGFEDEIAALRAAHAA
                     GDREAAIAAVSDRMVDAIDICGDAAHVATAVQEYRDAGVESPVIMPMPWGEDRVATVE
                     ATIDAVTG"
     gene            7679..7858
                     /locus_tag="PLZ72_07870"
     CDS             7679..7858
                     /locus_tag="PLZ72_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36248.1"
                     /translation="MTEVREPQSDESIRAVMQLIDQGLGDIADRSIVSTSEMADLLLD
                     VRSLLSRLDAEVHTN"
     gene            complement(7884..9290)
                     /locus_tag="PLZ72_07875"
     CDS             complement(7884..9290)
                     /locus_tag="PLZ72_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA36249.1"
                     /translation="MSPADPGPPDAPVERMSSMEAVMWVLDSDPRLASAFANLTILDR
                     APDRDELRTRMLAATTVVPRLRQKVVDGAPLSTPAWIDDTDFDIDHHLRWIHLGPNDL
                     DSVVADLSSRPFDRSRPLWEFVVIDGLPDGAAAMVQRMDHTLTDGEGGIRLSVQFLDL
                     ERHPERREPPAPTARPGRTDSNTTDTNTTDSADDPARGLVDQATRGLRAAANVPSALF
                     DMVRDSMRQVATPGRRSSLWTERSTERWFGRSTINLEDAKSAAHQLGGSVNDLFVTGA
                     LQAAARIHSAADAPVDELRVAIPLSQRSSGTAGGNAFTPAMALLPAGPMDVTERFTRV
                     HERLDRVKASRGSMGLEGPATVARLLPDSALVGIVGRSAGAIDFVCSNVRAAPFDLYI
                     AGAHLEANYPLGPLVNTAFNLTTMSYRGWLFLGLLVDRAAVADPDALLAAVDDAYSEL
                     LAAGGIGTRRTPDLSVTT"
     gene            complement(9287..>9547)
                     /locus_tag="PLZ72_07880"
     CDS             complement(9287..>9547)
                     /locus_tag="PLZ72_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNA36250.1"
                     /translation="HPHQLRTPQTSSFPSGHASAGACAATLLSRDLGVAPLWWTLAAA
                     VSWSRVHVGVHHGSDLVGGAALGAALGRLSTSVWPPRVPSAV"
     assembly_gap    9548..9647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9674..10522)
                     /locus_tag="PLZ72_07885"
     CDS             complement(9674..10522)
                     /locus_tag="PLZ72_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNA36251.1"
                     /translation="MALPLPTSLSPSKVSAFKDCALAFRFSAIDKIPEPPSMPATRGT
                     TVHRALELLFVEAPADRTPQRAQECLRTALEEMSTHEEYVGLELDDAAAADFAATSAR
                     MVDRYFTLEDPQSITPIGLELFLTAQLGSLTVRGIIDRLELDQDGELVVTDYKTGRAP
                     SPAQEQSRLGGVHFYSLLCERVLGRIPSKVQLIYLGDQPQVITTVPSEQSTRGVARKL
                     DAIWSAVERACDKEDFRPRRSALCNWCNFKAFCPAFGGDPSQAASLSIRPGPQRALDL
                     AAVDAG"
     gene            complement(10607..>11380)
                     /locus_tag="PLZ72_07890"
     CDS             complement(10607..>11380)
                     /locus_tag="PLZ72_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36252.1"
                     /translation="RDLLLVHATGFCAGVWGPLVEHLRGFRVTAIDVRGHGRSDAPDL
                     GPDGPGMSWAGTGDDVLAAIDALDLQDPVGAGHSMGGASLLIAELARPGTFGALWGFE
                     PIVMPPSIAVVDRPNPLADGARRRRDQFPSAEAAFANFAAKAPFDVLDPDALAAYVRY
                     GFEEHSDGTLSLRCRPEVEAATYEMGPRHPTFERLGAVPIPVTVLRGQDTPYSPAAFA
                     PAVADALPHGVLEEHPELGHFGPLQDPAAMAASISAALG"
     assembly_gap    11381..11480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11495..11872)
                     /locus_tag="PLZ72_07895"
     CDS             complement(11495..11872)
                     /locus_tag="PLZ72_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36253.1"
                     /translation="MTLPDDYLVRRCLDCWVVVGPSGLFVIGRAAGDPASASERTASM
                     AHTLRTRLADVMSWVPFVSAVVVGDRPRHDLACPMVELDELGSLLRDGGQQIGEGALQ
                     VLRHHVPGAVQQIELARGVASTR"
     gene            12073..13227
                     /locus_tag="PLZ72_07900"
     CDS             12073..13227
                     /locus_tag="PLZ72_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNA36254.1"
                     /translation="MEISRRTVLRWSGAAGMMAVGAVVLDSCAPSGTEEGADPNGLVL
                     LPGFTSRVIARANEPVADTGFVFRGFPDGAATFVDDEVPGGWYLAVNHEIPGSGGVSS
                     LRFAPDGTITDAFSICHGTTLNCAGGATPWGTWLTCEEYDLGRVQECDPTGRHGAIER
                     PALGAFAHEAAAVAPDGRVYLTEDRPDGGFYRFTPDTAGDLSRGLLEVATGSAAPGPV
                     TWVAVPDPSSSSTFSRRQVPTMIRFDGGEGIDMLGDRVLFSTKGDRRIWRYDPTDSTV
                     VVHHQAAEKGDGVLDAVDNLWVDDASSNVFVAEDGGNMEVVMLRPDSTVTAVARVVGQ
                     EGSEIAGPCFSPDGQRLYFSSQRAPVGPFNAPFGMTYEVSGPWDELLGRP"
     gene            complement(13231..>14682)
                     /locus_tag="PLZ72_07905"
     CDS             complement(13231..>14682)
                     /locus_tag="PLZ72_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA36255.1"
                     /translation="LPDIGKHFDASQTQLNMVAVGYSLGLAATVLWLGALGDRYGRRQ
                     MLLAGVALSLPASLIAAWAPSIHVLVAARVLGGVAAGMAYPTTLALITALWAGAGRTR
                     AIALWSAIGGAVSALGPLLSGVFLGRWWWGSVFLVTVPLIVVAIPMIRRVVPSHVNET
                     TETVDNLGGVISVVMVGAIIIAINFVVVPNLRTAAVIVGVIGLLLVAAFVVRQRRATN
                     PLYDLHIAARPIFWVAAVAGIIVFGSLMGAMFIGQQFLQNVLGYSTLDSGFAVIPAAV
                     FMVLCAPRSATLVEQQGARFTLLFGYAFVLAGFLFMLLVWGESSPYWHVGLAYSLVGA
                     GVGFAGTPASHSLTGAVPVTRAGMASGTADLQRDLGGAIMQSILGALLTAGYAASFSQ
                     LIASSPDADQISTSVQSELTKSFSSAANTAESYPQYSTQIIQAARDSFVQGQDWAYAA
                     GIIAVAVGAVLVFVFFPRHDREQRLLAEYAAEA"
     assembly_gap    14683..14782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14783..15428
                     /locus_tag="PLZ72_07910"
     CDS             <14783..15428
                     /locus_tag="PLZ72_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36256.1"
                     /translation="FDGAEGERRGPVVVAGVLTVGLPFIMPEFFRNDTGMGLAGLQLV
                     LLIAMFVLDPGRIDRRSRPVHLVRLALVASLAVGAAVSAMRMVWLIVERRRGTSSARD
                     LLWAGGLVWVHLVLAFAFLLWEMDGGGPGERVHRAPGPADLGFPQQDDPQRWPGWRPV
                     FVDYLHVAATGAISFSPADVVVLSHRAKAATFLLSASSLAILGLVVASAVNLLS"
     gene            15577..>15963
                     /locus_tag="PLZ72_07915"
     CDS             15577..>15963
                     /locus_tag="PLZ72_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA36257.1"
                     /translation="MAESSERYQRGAKKLREVYAGDVVDLPEGSFPFNDVMLRSLFAE
                     VWDRELLDVRSRRLLIMGVIATLGQTDTWKIQARASLRNRELSPDELRETLIMLAPYA
                     GYPNVAPLIMACEEVISTWTSDGEPGP"
BASE COUNT         2211 a         5391 c         5858 g         2203 t
ORIGIN      
        1 ccatggccga ccacacccct cccggcggag cgcgctgggg catgacgatc cccttcgacg
       61 gcgttccgct ccacgagcag cgcgactggt accgcgagct cgaggacctc ggctacaccg
      121 acgtgtggtc ggcggaggcg atgggcgccg atgccttcac gccgctggcg ctcgcctccg
      181 cgtgggcccc gtcgctgcgg ctcggcactg ccatcgtgcc ggcgttcacc cggggtccgg
      241 ccgtgctcgc ccagagcgtc gcgtcgttgg ccgacgccgc gccgggccgg ttcgccttcg
      301 gcatcggcac gtcgtcggac gtgatcgtcg gacgttggaa cgggatcccg ttcgaggagc
      361 cgtaccgcca ggtgcgcgac atggtccgct tcgtgcgcga ggcgctggcg ggcgagaagg
      421 tgtcggggac cttcggacgg tccgaggtca agggcttccg actgggcgtc aggcccgaag
      481 tcgatccgcc gatcctggtc gcggcgctcc gcgaggggat gctgcgcctg gctggccgcg
      541 agggcgacgg cgccatcatc aactggctgt cggccgagga cgtgtccacc gtcgcgccga
      601 tcgtgcagca ggccgccgcg gacgccggcc ggggacccaa ggaggtggtg gcccgcatct
      661 tcgtcgcccc caccaccgac cgggatctgg tcatgaaggt ggggcgctac gccatcgccg
      721 cgtacctgac cgtgccggtg tacgcggcgt tccacgagtg gatggggcgc ggcgagcagc
      781 tcgccgagat gtcccgccgc tgggccgacg gcgaccgggc gggggcgctc gacgccatcc
      841 ccgactcgct ggtcgacgag ctgatcgtct ggggcagccc cgagcagtgc cgcgaccaca
      901 tcggccgcta cgtcgccaac ggtgtcacca ccccggccat cgcggtgctg cccttcggcg
      961 tcgaccagcg ccaggccatc cgcgacctgg cccccgtagg ctgagccgat gaccgaccag
     1021 acggcgggag atccgcccgc gccggtgacg atcgtcgacg tggcgccccg cgacgggctc
     1081 cagtccgacg ggctgatcgt gtcgaccgag gccaagctcg agctgataga gcggatcgtg
     1141 cgagccggga tccgccgggt cgagacgacg tcgttcgtga accccgccag ggttccgcag
     1201 atggccgacg cggccgaggt catggccggc ctggccgccc ggcgtgcggc gtcacccgcg
     1261 ggcgacccgc tccgggaggc gtcgttcatc ggcctcgtgc tcaaccgccg gggactggat
     1321 cgggcgctcg aggccggctg cgacgaggtc aacgccgtcg tcgtcgtcag cgacaccttc
     1381 agcgagcgca accagggcac caccaccgac gagggcatcg tggtgtgggc cgagatcgcc
     1441 gaggcggccg ccgaggcggg ggtgcccgtc agcgtgacgc tggccgccag cttcggctgc
     1501 ccctacgagg gcgaggtgcc cgtcgaccgc ctggccgacg tcgtctcacg ctgcgtggag
     1561 gtcggcgggg tcgaggtggc actggccgac tcgatcggcg tggcggtccc caccgacgtg
     1621 cgcgcccgac tggcggtcgc ccgcgacgcc atcgccgcgg cgggcaccgg ccagcatctg
     1681 agggtgcact tccacaacac gcgcaacacg ggcctggcca acgtggccac cgcggtcgag
     1741 gagggcgtga ccgtggtcga ctcgtcgctc ggcgggatcg gcggctgtcc gttcgccccc
     1801 ggcgccaccg ggaacgtccc cacggaggac gtcgcctacc tgctgtcgcg gatgggtcac
     1861 gacacaggga tcgacctgga cgagctggtc ggggccgtcc actggatgca gtccgacgcc
     1921 ctgctcgacc gtgacacgcc cggagcgctg gcccgggccg gggtgttccc gcgccgctga
     1981 cgcgaaaatg ggcggatgcc ggtgtccgca ccatccctcc cgatggcacg ggcaccggca
     2041 tcggcggtcg ttgcccccag caccgaccgc tgtcggccag cgtcgctgac gaacgggcag
     2101 cggggcatcc gtaaccccta cccaacccct accgacctgg tctggattca ggagggtcgc
     2161 aggtggtcgc ggatctggtg cttgtcgacc ttgcccgagg cgttgagcgg caactcgggt
     2221 gcgctcacga tcacccgcgg tcgcttgaag ccggccatcc gaccctcggt ccagtcgacc
     2281 agagcccggg cccgggcgtc gaggtcggtc gcccgggtcg ggtcgtcggt gccgttccac
     2341 accacgacgg cgcacacccg ttcgccccag gtgtcgtcgg gcagaccgac cacggcgacc
     2401 gcagcgacgt cggggtgttc ggagacgatg tcctcgacct cgcgggacga cacgttctca
     2461 ccgcccgaga tgatgatgtc cttctttcgg tcgacgatgt acaggtagcc ggcctcgtcc
     2521 aaccggccga ggtcgccggt gcggaaccac tcgccgtggt gcgactcgac cgtggcgccc
     2581 ggatcgtccc agtagcccgc gcagacctgg tcgccgcgca ccaggacctc gccgacgtcc
     2641 ccggcgggca cctcttggcc gcggtcgtcc gcgatgcgga tctggaccag gggggcgggc
     2701 cggccggccg cgctcagcag ccacggctcg tcggctccgg cccgccggtg ctcgtcgggg
     2761 cccaggaaca cggcgttgcc cgacagctcg gtcatgccgt agccctgggc caggcccacc
     2821 tcgggcagct cgctcagcgt gcgacgcagc aggtcgagcg gcatcgccga ggcgccgtag
     2881 ccgatgcggc gcaggctcga gaggtcctcg acgctgcgtt cggggtgctc caacagcatc
     2941 gccagcatgg tgggcgccag gctgacggtg gtgaccgact cgtcacggat ggcggtgagg
     3001 acctcgtcgg ggtcgaagcg ggggagcagc acgaccggtc ggcggcccag gtggtgcacg
     3061 acgacgttgt aggcggcgac gtggaacagc gggaacggga acaggtagac gtcgtcgtcg
     3121 gagatcgggc gggcgagcac cgtgttggtg gccgcggcca ccagggaacg gtgggtgagc
     3181 aacgcgccct tggcccggcc ggtggtcccg gaggtgtgga tcaaccacac gtgggtgtcg
     3241 tcgtcgaggc ctgatcgtgg cggttcgggt tcccgggcga acagctcgct gatgtcccgg
     3301 gtccccgacg ggagctcggc tccgtcgtcg gtcacggggc cgtcgtgggt cgcgacgccg
     3361 tcgaggcagt accagcggcg gacgtcgtgc agctcgtcgg cgtgctcggt cagccgggcg
     3421 agctgttcgg ccgagccgat gaccaccgat gccgagctgg ccttgagcat cgccgcctgt
     3481 tcggccgcgg tgtggcggat gttgcccagc accagcagcg ctccggcgcg gggcacggcg
     3541 tagagcgcca tgaccagctc gggtcggttg tcggagaacc acgcgatccg ctcgtcgcgg
     3601 gcgacgacgt gagcgagggt cgtccccatg cgggcgacgt cccgttcgag ctcggagaag
     3661 gtccagcggc ggtcgccggc cacgagggcg tcgacgtcgg gcgaggtgcc cgcggcgaac
     3721 tcgatcacct ggtgcaacag catcgtcgga ccctaccggc ccgtgggcag ccgtcggtcg
     3781 ggcaactgct ggacggagca gctactcgtc gaggccgacc gggttcccgt cgacgatctg
     3841 gtccaggtac tccgacaggc cgtgtccgac cgtggcggtc gggtcgtcgc cgagcgcccg
     3901 gtcgagctcc cagcgggtga gcccttcgga gatgcgggtg acccgctcgt cgcggcggtt
     3961 gcgcaacggg atcaggttga gcacatcgcc ggtgacggtg cgctcgatgc ccggggtgat
     4021 cccgtcggtc cccaggcgga cggtggagaa ggtggcgtgg atccgcgagt ggtgggagtc
     4081 gtcgccgacc cactcggtgc gcagcgtcac gtcgccgacc aggtgcagct cccgaccgtc
     4141 ccagacgaag ccgctgcggg tcccgcggct gtcgggtcgg gcgatgcggc tgagcatcag
     4201 gccggtgccg tcggagaagt tcatggtgag ccaccggtag taccaggggg cctgccaggt
     4261 gcgggggccc cacgagtggt cccgcagccc gagcccggcg acgctccagg tggagtcgcc
     4321 gacggcgatc gtcccggtgc cgcgcacgag ctgctcgtag tggcccttgg cgaactcctc
     4381 gcccggccgc tcgtggcgct cctcgggctc accgccgaag gggcgcgaga tgccctcgta
     4441 ggtgagctgc acccgggcgt ccgcgtaggg gttctcggtg aaggcccggc gcgggtgggc
     4501 catgtcgagg ggctgctcga gcagcaccac ctgcccgtgg tagccgacgt cgatccggcc
     4561 gccacccgcc ccctccgcgt gctcgacgtc ggtgcggaac ggttcgacga cgtcgatcgt
     4621 cgctccgccg gcatcgaagc ggtcgttgga gtcgatgtcc gctcgacgga acatgaagcc
     4681 gacccgtccg tcgggcaggt acaggcacac cgtcatctcg gcgtggccct cgttgggacg
     4741 gttgccgatc cgcacgaacc cgcccagccg agcgtcgggg tcgtacaggt tgaagtacat
     4801 cgactcgttg aaggtgggct cggggcccgg gtgcatgtac tcgtcggcgg gctcgaggcg
     4861 caccttcatg gtcgtccccc tggtcgatcg gtcgctgcgg cgaatctagg tcagcgcagc
     4921 tccgtcgccg cccggaggtg ccgaacgagg tcgtcgacgt cggtgtgcgc gttcagtccg
     4981 ctcgggttcg gcatcacgta cacggggcac ccgccgagcg tcgcggcctg cggcccgagc
     5041 cgggcggagc gcattccggt cgcggcccgc cacccggtga tcccgagcac gcacaccgcg
     5101 gtcggctgca gccagtcgca gaggacctcg aggcgttcga gtccgtcgcg gaactcctcg
     5161 gctcgcagct cacccgccgt ggcggtggtc cgcttgacga gatcggtcat gccgatgccg
     5221 gcgtggtgca gcaggtgcag cgggtcgcgg tcgacctcgg ccaacccggc ggcggcgaga
     5281 gcgggccacg cccggttgcc cggccgggcg aagccgacgc cgacctcggc tgcgtacggc
     5341 gacgggttca gaccgaccag cagcagcctc atgcccgggc tgacggtgtc ggcgagcgtg
     5401 tgctcccggc gcagggtgag cgtcacacga tcgtcgttcg accgttcgtc gaggactgcg
     5461 aagcccgcac ccgtcacgac gtggtggagg cgccggcgcg gccaccggtc cgggtccgcg
     5521 ctgacggcga tccctacctc ggcgccgaca tccaggctct cgtggagatc ccgccaggcg
     5581 agagggcacc gtcgggggtc gacagcgacg tggtcgtgca ctgcgggccg tgatggcacc
     5641 gcggtcagcg gccgaggagc ttggcctgca accgcctcat ggcgcccagc caccggtcgc
     5701 ggtcggtggc tcgggcccgt tcgtagtcgg cgacctcggg gtgcggcagg atcaggaagc
     5761 gttcgtcggc cagaccctcg accacggtgt cggcgacctc ctcgggttcg aggatgcgct
     5821 gcgccttgac gacgtccgcg gcgagcgctc cgtcggtggg cgagtcggag tccccgaaca
     5881 gcagcggggt ccggaccccc tgcgggcaca ggcacgccac gtggagcccc cggtcgccgt
     5941 aggtgaccga cagccactcg gccagaccga ccgcggcgtg cttggtggcg gtgtaggggg
     6001 catctcccag gttggtgagc aggccggcgg ccgacgcggt gatcaacagg tggccctcgc
     6061 ccgcctcgag ccacgccggg agcagcgccc gcgccgcgta gacgtgggcc atcacgttga
     6121 cgtgccagat ctgctcccag gccgggtccg gtgcctcgac gcccatcccg gtgccgatcc
     6181 cggcgttggc gcagaacagg tcgagccgtc cgtggtgcag gaacacctcg gcgatcatcg
     6241 ctcccacctg ggcctcgtcg gccacgtcga tcgcccggga tgtcacctcg gtccccggcg
     6301 ccgcctcgcg gacgtcgtcg gccaccgacg cggcggcgtc gccgttgatg tccacgacca
     6361 cgatcgcggt cggggcctcc accgcgaacc ggcggcacat cgccgcgccg atcccgctgc
     6421 cgccaccggt caccacgatc acccggtccc tgagctccat ggcgtccgac cctacgccgt
     6481 gccggtcgtc cgtcggggcg gatcggctca gccggtgacg gcgtcgatgg tggcctcgac
     6541 ggtggcgacc cggtcctcgc cccacggcat cggcatgatc accggtgact cgaccccggc
     6601 gtcgcggtac tcctgcaccg ccgtggccac gtgggcggcg tcgccgcaga tgtcgatcgc
     6661 gtcgaccatc cggtccgaca ccgcggcgat cgccgcctcc cggtcgccgg cggcgtgcgc
     6721 ggcacgcagc gcggcgatct cgtcctcgaa cccggctcgc tcgaacgccc gggcgtaggc
     6781 gtcgaccacc gcgtaggaga acaggtcctt gcgtcccgac ggtcgggccc gatccgggtc
     6841 gcagaccccg acgtgcacgt aggcgtggat ccgacacgaa cccggttctc gctgggcgaa
     6901 ctgctcaccg gctcggacct gctccacgca ccacggcacc gccgatgccg gcaggtagtt
     6961 caacaggacc ccgtcggcca gctcgccggc caggcgcagc atccgctggt tgagcgcgcc
     7021 gagcaccagc tgcggtcgcg ggccgtgcag acggaccccc aggcggaacc ggttcgccga
     7081 gtagaactcg ccgtcgtagg tcaccggctc gccgctcaga caggcggcgg tcagctcgag
     7141 gaactcgcgg acctgggcga cgggtcgggt cccgtactcg gcgccgtgcc agcggcccac
     7201 cacgaccggc gaggagatgc cgacgcccag cagcacctcg cgatccggcg ccagggcctg
     7261 caacgtggcg gctcccatgg cggccacctg cggggtgcgc agctggaggg cgagcacccc
     7321 ggtgcccagg tcgagaccac cgacgcctgc cgcgaccgcg ccgagcgtcg cgaacgcctc
     7381 ggtgccgacg gtctccgcgg tccagaacga cgagcagccg gcgtcgcggg ccgtccgggc
     7441 catggccagt cccacgtcca ggccgagggc ctggaagctg gcgaaggtga gccccacggg
     7501 ccggtccggg gcgatgctgg cggcgtcggt cgacatggca cggaacggta gccgggggca
     7561 ccaacggggc gatcctgagg tgtggttcgg gaaacggcgc ccgcgtcgtc ccaggcgcgg
     7621 ggtacccttc tggcctgagg cgaacgagtc ggcctgaggc gaacgaggag gacatgagat
     7681 gaccgaggtc agagagccgc agtccgatga gtccatccga gcggtcatgc agctgatcga
     7741 ccagggtctc ggtgacatcg cggaccggtc gatcgtgtcg acctccgaga tggccgacct
     7801 cctgttggac gtccggtcgt tgttgtcccg cctcgacgcc gaggtgcaca cgaactgagt
     7861 gcctggccct cgcgggcagg gtgtcaggtc gtgacgctga ggtcgggggt gcggcgcgtg
     7921 ccgatcccgc cggcggccag cagctcgctg tacgcgtcgt cgaccgcagc gagcagggcg
     7981 tcggggtcgg cgacggcggc ccgatcgacc agcagaccca ggaacagcca cccccggtac
     8041 gacatggtcg tcaggttgaa cgcggtgttg accagcggtc ccagcgggta gttggcctcg
     8101 aggtgcgctc cggcgatgta caggtcgaac ggtgccgccc gcacgttgga gcagacgaag
     8161 tcgatcgcac cggcgctgcg tccgacgatg ccgaccaggg ccgagtccgg caggagtcgg
     8221 gccacggtcg ccggaccctc gagccccatc gacccgcgtg aggccttgac ccgatcgagg
     8281 cgctcgtgca cccgggtgaa gcgctcggtg acgtccatcg ggccggcggg cagcagggcc
     8341 atcgcggggg tgaaggcgtt gcctccggcg gttccggagc tgcgctggct cagcgggatg
     8401 gccacccgaa gctcgtcgac cggtgcgtcc gctgcggagt ggatccgggc cgcggcctgt
     8461 agtgctccgg tcacgaagag gtcgttgacg ctgccgccca gttgatgcgc ggccgacttg
     8521 gcgtcctcga ggttgatcgt gctgcgcccg aaccagcgct cggtggatcg ctcggtccac
     8581 agcgatgatc gacgtccggg ggtggccacc tgccgcatcg agtcgcggac catgtcgaac
     8641 agggcgctcg gcacgttcgc ggcggcgcgc agaccccggg tcgcctggtc gaccaacccc
     8701 cgagccgggt cgtcggcact gtcggtggtg ttggtgtcgg tggtgttgct gtcggtgcgg
     8761 ccgggccgtg cggtcggtgc cggcggttct ctccgctccg ggtgccgctc caggtcgagg
     8821 aactgcaccg acaggcggat gccaccttcg ccgtcggtga gggtgtggtc catccgctgg
     8881 accatcgcgg cggcgccgtc ggggagtccg tcgatgacga cgaactccca cagtggccgg
     8941 gaccggtcga acggccggga cgacaggtca gcgacgaccg agtccaggtc gttcggaccc
     9001 aggtggatcc accgcaggtg gtggtcgatg tcgaagtcgg tgtcgtcgat ccaggccggc
     9061 gtgctgagcg gtgcgccgtc gacgaccttc tggcgcaacc gggggacgac cgttgtcgcg
     9121 gcgagcatcc gggtccgaag ctcgtcgcgg tccggggcac ggtcgaggat ggtgaggttg
     9181 gcgaaggccg aggccaggcg agggtcgctg tcgagcaccc acatgaccgc ctccatcgac
     9241 gacatgcgtt cgaccggtgc gtccggcggt cccggatcgg cggggctcac accgccgacg
     9301 gtacccgcgg gggccacacc gaggtgctga gcctgccgag ggcggcaccg agcgccgctc
     9361 cgccgaccag gtccgaaccg tggtgcaccc cgacgtggac ccggctccac gacaccgcgg
     9421 cggcgagggt ccaccacaga ggcgccacgc cgagatcgcg gctgagcagg gtggccgcgc
     9481 acgcgccggc cgatgcgtgc ccgctgggga acgacgacgt ctgcggcgtc cggagctggt
     9541 gcgggtgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc cgccggtcgc
     9661 gccgctgccc ggttcatccg gcgtcgacgg cggccaggtc gagggcgcgc tgcggtcctg
     9721 gtcggatcga caacgacgca gcctgcgacg ggtcaccgcc gaacgcaggg cagaacgcct
     9781 tgaagttgca ccagttgcac agcgcggacc ggcgagggcg gaagtcctcc ttgtcgcacg
     9841 ctcgttcgac cgccgaccag atggcgtcga gcttgcgggc cacgccgcgg gtggactgct
     9901 ccgagggaac cgtcgtgatg acctgtggct ggtcacccag gtagatgagc tgcaccttgg
     9961 acgggatgcg gccgaggacc cgttcgcaca gcagcgagta gaagtgcacg ccgccgagtc
    10021 gggactgttc ctgcgcgggc gacggggcgc ggccggtctt gtagtcggtc acgaccagtt
    10081 cgccgtcctg gtcgagctcc agccggtcga tgatgccccg gacggtgagc gaaccgagct
    10141 gggcggtcag gaacagctcg aggccgatcg gcgtgatcga ctgcgggtcc tcgagggtga
    10201 agtagcgatc gaccatccgg gccgaggtgg cggcgaagtc ggctgcggcg gcgtcgtcga
    10261 gctccaggcc gacgtactcc tcgtgggtcg acatctcctc cagggccgtg cgcaggcact
    10321 cctgggcccg ttggggcgtg cggtcggcgg gagcctcgac gaagaggagc tcgagggccc
    10381 ggtgcacggt ggtgccgcgg gtggcgggca tggacggcgg ttcggggatc ttgtcgatgg
    10441 ccgagaaccg gaacgcgaga gcacagtcct tgaacgcgga caccttcgac ggcgacaggg
    10501 aggtgggcag cggcagggcc atggccggaa cactacggcg cccccctgac agcggggacg
    10561 tggaggtgcc cgcaggccac cttcgggaag gcccctcggt ccgggttcag ccgagagcgg
    10621 cggagatcga cgccgccatg gcggccgggt cctgcagcgg gccgaagtgc cccagttcgg
    10681 gatgctcctc gaggaccccg tgggggagcg cgtcggccac cgcgggggcg aacgcggcgg
    10741 gcgagtacgg cgtgtcctgg ccgcggagga ccgtcaccgg gatcggcacg gcgccgagtc
    10801 gctcgaatgt ggggtggcgg ggccccatct cgtaggtggc ggcctccacc tcgggtcggc
    10861 agcgcaacga cagcgttccg tcggagtgct cctcgaaccc gtagcgcacg taggcggcca
    10921 gcgcatccgg atcgaggacg tcgaacggcg ccttggccgc gaagttggcg aacgccgcct
    10981 cggcggaggg gaactggtcg cgtcggcgcc gggccccgtc ggcgagcggg tttggacggt
    11041 cgacaactgc gatcgagggt ggcatgacga tcggctcgaa cccccacagg gcgccgaagg
    11101 tgccggggcg ggcgagctcg gcgatcagca gcgaggctcc ccccatcgag tgtccggccc
    11161 cgaccggatc ctggaggtcg agcgcgtcga tcgcggccag gacgtcgtcg cccgttcccg
    11221 cccacgacat ccccggaccg tcggggccca ggtccggggc gtcggaccgg ccgtggccgc
    11281 gcacgtcgat cgcggtgacc cggaagcccc gcaggtgctc gaccagcggg ccccacaccc
    11341 cggcgcagaa gccggtggcg tggaccagca gcaggtcgcg nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn gagggggaga gatgtcagcg ggtgctggcg acgccgcggg
    11521 cgagctcgat ctgctgcacg gcgccgggaa cgtggtggcg caggacctgc agcgctccct
    11581 cgccgatctg ctggccgccg tcgcgtagca gcgatccgag ctcgtccagt tcgaccatcg
    11641 ggcaggccag gtcgtgacga ggacggtcac ccaccaccac cgcgctcacg aatgggaccc
    11701 aggacatgac gtcggccaac cgggtgcgca gcgtgtgggc catcgatgcg gtgcgctccg
    11761 acgcggaggc ggggtcgccc gccgctcggc cgatgacgaa cagcccggag gggccgacga
    11821 cgacccagca gtccaggcag cgtcggacca ggtagtcgtc gggaagggtc atgacgaccc
    11881 ggtcctccga cgtgttcggg cgcggtctga acaccttcct tccatcggca gcaccggact
    11941 ggttgttgag cggaaccgcg tcggtcagcg ccgggtgtcg gccggcgacg atgtctacgg
    12001 ggcggcctgc accggtactc tcgccaggga tcggtcggct gagcggtcga ccggtggacg
    12061 gggagacgtg acgtggagat cagtcgtcga acggtgctgc gctggtccgg agccgcgggg
    12121 atgatggcgg tcggcgcggt ggtcctcgac tcgtgcgccc cgtcgggcac cgaggaggga
    12181 gcggacccca acggcctggt gctgctgccc gggttcacca gtcgggtgat cgcccgggcc
    12241 aacgaaccgg tcgccgacac cgggttcgtg ttccggggct tccccgacgg cgccgcgacc
    12301 tttgtcgacg atgaggtccc cggaggctgg tacctggcgg tcaaccacga gatccccgga
    12361 tcgggcggcg tgtccagcct gcgcttcgct cccgacggca ccatcaccga cgccttctcg
    12421 atctgccacg gcaccacgct caactgcgcc ggcggcgcga cgccgtgggg gacgtggctg
    12481 acgtgtgagg agtacgacct gggtcgggtc caggagtgcg accccaccgg acggcacggt
    12541 gcgatcgagc ggccggcgct cggggcgttc gcccacgagg cggcggcggt cgcccccgac
    12601 ggccgggtct acctgaccga ggaccgcccc gacggcgggt tctaccggtt cacccccgac
    12661 accgccggcg acttgtcccg gggtctgctc gaggtggcga ccggctcggc ggcaccgggt
    12721 ccggtgacgt gggtggcggt ccccgacccg tcgtcgtcga gcacgttcag ccgtcggcag
    12781 gtcccgacca tgatccgctt cgacggcggc gagggcatcg acatgctcgg cgaccgggtc
    12841 ctgttcagca ccaagggcga tcgacggatc tggcgctacg acccgaccga ctcgaccgtg
    12901 gtcgtccatc accaggccgc cgagaagggc gacggggtgc tcgacgcggt cgacaacctc
    12961 tgggtcgacg acgcctcgtc gaacgtgttc gtggccgagg acggcgggaa catggaggtg
    13021 gtcatgctcc ggcccgactc cacggtcacc gcggttgccc gcgtggtcgg ccaggagggc
    13081 tccgagatcg ccgggccgtg cttcagcccc gacggccagc gcctgtactt cagctcgcag
    13141 cgggctccgg tcgggccctt caacgccccg ttcggcatga cctacgaggt gagcggcccg
    13201 tgggacgagc tcctgggtcg cccctgaccg tcaggcctcg gcggcgtact ccgccaggag
    13261 gcgctgctcg cggtcgtggc gggggaagaa cacgaacacg agcacggcgc cgacggcgac
    13321 ggcgatgatc cccgccgcat aggcccagtc ctggccctgc acgaacgagt cccgagcggc
    13381 ctggatgatc tgcgtggagt actgggggta gctctcggcg gtgttcgccg cgctggagaa
    13441 gctcttggtc agctcgctct gcaccgaggt gctgatctgg tccgcgtcgg gggacgaggc
    13501 gatcaactgc gagaacgacg ctgcgtaacc cgcggtgagc agcgccccca ggatcgactg
    13561 catgatcgct ccgcccaggt cgcgttgcag gtccgcggtg cccgaggcca tgccggcccg
    13621 ggtgaccggg accgcaccgg tgagcgagtg cgacgccggg gttccggcga accccacgcc
    13681 cgccccgacc agcgagtagg ccagaccgac gtgccagtac ggcgagctct ctccccacac
    13741 cagcagcatg aacaggaagc cggcgagcac gaaggcgtag ccgaacagca gggtgaagcg
    13801 ggcgccctgc tgttcgacga gcgtggccga gcgcggtgcg cacagcacca tgaacaccgc
    13861 ggcggggatc acggcgaagc ccgagtcgag cgtcgagtac ccgagcacgt tctgcaggaa
    13921 ctgctggccg atgaacatgg ctcccatcag cgacccgaac acgatgatcc ctgccaccgc
    13981 agcgacccag aagatcgggc gagccgcgat gtgcaggtcg tagagggggt tggtcgcccg
    14041 gcgctgccgc acgacgaacg ccgcgacgag caggaggccg atcaccccga cgatcactgc
    14101 cgcggtacgc aggttgggca ccaccacgaa gttgatggcg atgatgatcg ccccgaccat
    14161 cacgaccgag atgacgccgc cgaggttgtc gacggtctcg gtcgtctcgt tgacgtgcga
    14221 cggcacgacc cgacggatca tcgggatggc gacgacgatg agcggaacgg tgaccaggaa
    14281 caccgatccc caccaccacc tccccaggaa cacacccgac agcagggggc cgagagcgga
    14341 gacggcaccg ccgatcgccg accagagtgc gatcgcccgg gtccgaccgg cgcccgccca
    14401 caacgccgtg atgagcgcca gcgtggtcgg ataggccatc ccggcggcga ccccaccgag
    14461 gacgcgggcg gcgacgagca catggatgct gggagcccag gccgcgatga ggctcgcggg
    14521 cagagagagg gcgacgccgg cgagcagcat ctgccgacgg ccgtagcggt cgccgagggc
    14581 acccagccag aggaccgtgg ccgccaaccc gagcgagtag ccgaccgcca ccatgttgag
    14641 ctgggtctgc gacgcgtcga agtgcttgcc gatgtcgggc agnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nncttcgacg gggccgaggg ggagcgacgg gggccggtgg
    14821 tggtggccgg tgtgctcacc gtgggcctgc cgttcatcat gccggagttc ttccgcaacg
    14881 acaccgggat gggcctcgcc gggctgcagc tggtgctcct gatcgccatg ttcgtgctcg
    14941 atcccggccg gatcgaccga cggtcccgac cggtgcacct ggtccgactc gcgctcgtcg
    15001 cctcgctggc ggtgggcgcc gcggtgtccg cgatgcggat ggtgtggttg atcgtcgagc
    15061 gccgacgcgg cacgtcgtcg gctcgtgacc tcctgtgggc cggcgggctg gtgtgggtgc
    15121 acctggtgct ggcgttcgcg ttcctgctgt gggagatgga cggcggcggg ccgggggagc
    15181 gggtccaccg ggcgccgggg cccgcggatc tgggcttccc ccagcaggac gacccgcagc
    15241 gctggcccgg gtggcgaccg gtgttcgtgg actacctgca cgtggcggcc accggggcga
    15301 tctcgttcag cccggccgac gtcgtggtgc tgtcgcaccg ggccaaggcg gcgacgttcc
    15361 tgctgtcggc gtcgtcgctg gcgatcctcg gtctggtcgt ggccagcgcg gtcaacctgc
    15421 tcagctgacc cggtcgctcc ggtgttcgcg tgtgggatcg acctcccacc ggtcgttgtc
    15481 gcacgcgaac gtcgggttcg ctgcgtccgc tgatcacgtc gcggtggggc gacggtggtg
    15541 taagagaacg ccaccgatcc gaggagggca gttccgatgg ctgagagcag cgagcggtac
    15601 cagcgggggg cgaagaagct gcgcgaggtg tacgcgggcg acgtggtcga cctgcccgag
    15661 ggcagcttcc cgttcaacga cgtgatgctg cggagcctgt tcgccgaggt ctgggaccgg
    15721 gagctactcg acgtgcggtc ccgacggttg ctgatcatgg gggtcatcgc caccctcggt
    15781 cagaccgaca cgtggaagat ccaggcccgg gcgtcgttgc gcaaccggga gctgagcccc
    15841 gacgagctgc gcgagacgct catcatgctg gccccctacg ccggctaccc caacgtggcg
    15901 ccgttgatca tggcctgcga ggaggtcatc tcgacctgga cctccgacgg cgagcccggc
    15961 ccc
//
LOCUS       DAOQOD010000063        15937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1303_length_15937_cov_12.123473, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000063 DAOQOD010000000
VERSION     DAOQOD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_15937_cov_12.123473"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLZ72_07920"
     CDS             complement(<1..318)
                     /locus_tag="PLZ72_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36258.1"
                     /translation="MVDLTIAAIPFFWGSMGAEALYLKRARDAGAAPSAGDYEWRDTA
                     TSLGMGVASLVVPAVTRPIFKRFDVVDGRYRRTVLGVAGAAAVAAVAADAIARSRELA
                     AGEV"
     gene            complement(374..1252)
                     /locus_tag="PLZ72_07925"
     CDS             complement(374..1252)
                     /locus_tag="PLZ72_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HNA36259.1"
                     /translation="MQPTTAPTTTAPPETTLDWTTVRSGPIDGFDITLRFAATLPADQ
                     VQPVIDSFNAAATRWEQVITADLAPYSPSGFDGCDDAGPVGTIDDVVIDVSVGPGDGP
                     GGVLGSAGFCLRTGTLARAGIMNFDAADVGTWLANGRFDRIVLHEMGHVLGIGTMWPY
                     AGLRDTTEPTDPRFTGPGAVAEWNTLSASTDTGVPIEAGGGPGTALAHWRESTFRSEL
                     MTGWISSGPSPLSRLSIASLADLGYTVDVDGADPYTLGSVMTDALFPGTEAHGASAGL
                     GSGFILLTPDGPLGSD"
     gene            complement(1461..2195)
                     /locus_tag="PLZ72_07930"
     CDS             complement(1461..2195)
                     /locus_tag="PLZ72_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA36260.1"
                     /translation="MSDTPRSEPVLLVDDDAGVRTLTFNRPGALNAFDQELWYAAAAA
                     LEGAAADDHVRCVLVTGNGRAFSAGQDLGEMADPTVFEGQEPGYQRFMPVLESFPKPI
                     VAAVNGVGVGIGLTMLLHCDIVLMSDQARLKVPFISLGVTTEASASLLMPATMGWQRA
                     AEVLFTEPWIDADQAVADGIALRAVPHDRLLDEARALAGHIGGLPLGPLLATKALMQA
                     GRADAVAAARTREVAEFEHLVRAMTG"
     gene            2255..3622
                     /locus_tag="PLZ72_07935"
     CDS             2255..3622
                     /locus_tag="PLZ72_07935"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA36261.1"
                     /translation="MSTTPADLAAASNAESTAADRRYVFHSWSAQDLISPMPIAGGDG
                     AWFWDNEGNRYLDFASQLVNLNLGHQHPDMIAAIKAQADTLCTVAPPFANDVRTEAAR
                     LVAEHAPGALNRVFFTNGGAEANENAVRMARLHTGRQKVLAAYRSYHGATATAITLTG
                     EPRRWASEPGAPGVVHFFGPYPYRSSFGAATDEQECERALAHLADVVQMEGPHTVAAI
                     VLESVVGTNGVLVPPDGYLAGVRELCDRHGILMIADEVMVGFGRVGEWFAVDRWGVEP
                     DLLTFAKGVNSGYVPLGGVLISDDVAATFAERPYPGGLTYSGHPLACATAVASIGIFE
                     RDGIVERSRRLGDDVIAPALVEMADRHPSVGEVRGMGCFFAIELVRDRGTREPLVPFN
                     AAGADAAPMVEVMSACKSGGVWPFAHFNRLHVAPPLVISEDDLRSGLAVIDAALDVSD
                     RLVGG"
     gene            3833..4036
                     /locus_tag="PLZ72_07940"
     CDS             3833..4036
                     /locus_tag="PLZ72_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNA36262.1"
                     /translation="MATGTVKFFNSEKGYGFISRDDNSEDIFVHFSQIAGSGYRNLEE
                     GQKVEFEVGPGRKGDEAQNVRAV"
     gene            4203..>5314
                     /locus_tag="PLZ72_07945"
     CDS             4203..>5314
                     /locus_tag="PLZ72_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36263.1"
                     /translation="MAQDDTPTSEQPATGDAPDSELARLRAENERLQRELEATGPAGA
                     AAGPRHRGRWFASWVLIVVGSLLVPVSVTSLWLNRTITDTDRYVETVAPLIRDQDIQR
                     AIEARLEKVLYAQVDIQAEVANVLPERAAPLAAPITAGLKNLISEVIHRVVTSDKVAE
                     LWDRANRIAQEQVEQVLTVSNGRKGVVSVDLTDTLKEVQKQLTDAGVPFVGNIAVPKV
                     DLDVMQSDTIAQVQTAFGIFDRLATILPWLTLLILGAGILAAPDRRKGLVHAASGWVI
                     GSLLLLVGVALGRMLYLNALPPGVSMPANETFFDTISRFLRGGGRTVLVLGLLILVVA
                     LVTGPSGPAVRFRTAVSRLVGAAGSGVDRTGVDLGPV"
     assembly_gap    5315..5414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5445..6620
                     /locus_tag="PLZ72_07950"
     CDS             5445..6620
                     /locus_tag="PLZ72_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA36264.1"
                     /translation="MHDEPVIDADNHYYEALDAFTRHRDPAWADRTVRWCDIDGRKYH
                     VVGGRVSRAVTNATFDPVVRAGAMHDYFRGNPDGRDPLSFLTDREPIRPEYRDRDARI
                     ATLDEQGLAACWMFPTLGMIYEELLTADPEACCVTFRAFNRWVQDDWGFAHRDRIFAA
                     PYITLADPDWAVDELQWALDAGARLIVMRPAAPTTVLGRVSPFDPRFARFWGLLDESG
                     VPLVLHAGDGGVSSNGYAPDGFSASFSGGFTPSIKFFAIEQAIKDFLLSMLLENHLVR
                     YPNLRIVSVENGAEFLPDLFRKARSMAHKVAGWFPDDPVDMFRAQVWINPFWEDDVVS
                     VVEQMGADRVVFGSDWPHIEGMPRPLDYLPEVKELSAADQRLVLHDNAAGLLAPATP"
     gene            6696..8405
                     /locus_tag="PLZ72_07955"
     CDS             6696..8405
                     /locus_tag="PLZ72_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA36265.1"
                     /translation="MADLTIRNGLLVDGTGAPARPADVVVDGDRIVGVTEPGQGSAGH
                     REIDADGRLVTPGFVDIHTHYDGQATWDPEMTPSGWHGVTTVVMGNCGVGFAPADPAR
                     RDWLIQLMEGVEDIPGTALAEGMSWNWESFPEYLDELDSMPRVMDVAAQIPHGAVRGY
                     VMGERGAANEPASAEDIALMSKLVEEGLRAGAVGFTTTRTVLHRAKDGELAAGTTAEV
                     EELLGLGDALGAAGTGVFGVASDMIDPTPEFDWMIEISRRTGRPVTFGCLQNDVRPEQ
                     WRELLDRAADANAAGARIVPQVAGRPACLLFGFESSVHPFITHRAYREIAHLPLAERV
                     AELRTPERREAILSEEVTRSGFGAYLLSSFQKLFPLGDPPDYEPAPERSVAAIAAREG
                     RRPEEVTYDLMLGRDGTELLYFPMLGYSHGDFEAMREMLDRDDTVLGLGDGGAHCGIL
                     CDASLPTYMLTHWSRDRDRGARFTLEQVVAMQTSRTAALYGFDDRGVVAPGLRADLNV
                     IDLDGLRLSPPEMVHDLPAGGRRLIQRATGFAATICAGEPVRLDDESTGSRPGRLVRS
                     ARA"
     gene            8449..>9290
                     /locus_tag="PLZ72_07960"
     CDS             8449..>9290
                     /locus_tag="PLZ72_07960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA36266.1"
                     /translation="MEVLAAVYGLEDAGSEARRLEAAGVDGVFTFEGPHDVFIPLALA
                     AAATDLSVMTNVAIAFPRNAVHMAHAAWDLHVLSGGRCTLGLGTQVRPHIEGRFGVDF
                     DRPVERMRDAVEAVRSVFRTWQDGVPLEHRGPFRTHTRMSPMFSPPPHPAGPPRIAVG
                     ALGPRLTAMAAEVADALAVMPVTSEEFFTEVTLPAVDRGRTDRPSRLEQLEVLPELIV
                     CCGRDAAEQAVADAGCRALLGFYVSTPAYRPVFERAGRGDLQPRAQQLTREGRWDALG
                     DLID"
     assembly_gap    9291..9390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9391..9624
                     /locus_tag="PLZ72_07965"
     CDS             <9391..9624
                     /locus_tag="PLZ72_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNA36267.1"
                     /translation="DAIDGTFDGVLAVRVSAPPVDGRANEAVRRLLADAFGVARSEVT
                     IVGGHGSRRKRVEIDGDEAGLRARLAELADPAG"
     gene            complement(9700..10107)
                     /locus_tag="PLZ72_07970"
     CDS             complement(9700..10107)
                     /locus_tag="PLZ72_07970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA36268.1"
                     /translation="MTDPVATESLRELMPLGALLQFSVLSSTAEQVVLSAPWQPEHCT
                     TGGILHGGYMMALADAAGALCAFKNLPPDTWTSTIESKTNMIRAVASGTVTATSTPVH
                     VGRTTIVVQTDLTRDDGKLAARTTQTQAVMSAS"
     gene            10174..11166
                     /locus_tag="PLZ72_07975"
     CDS             10174..11166
                     /locus_tag="PLZ72_07975"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNA36269.1"
                     /translation="MSDRRPEPRSDELDPSGPGGSAPAVSEIFDPEAWAPVPGFEDLD
                     DITYHRAVDVGVVRVAFNRPEVRNAFRPRTVDQLYRVLDHARQSPDVGCVLLTGNGPS
                     PKDGGWAFCSGGDQRIRGRDGYRYTVEDESGDPGLTAEAIDPARSGRLHILEVQRLIR
                     VMPKVVICVVPGWAAGGGHSLHVVSDLTLASREHARFKQTDADVASFDGGFGSAYLAR
                     QVGQKFAREIFFLGDEYSAEDAHRMGMVNAVVDHQDLERVALDWGRKVMGKSPTAQRM
                     LKFSLNLIDDGLVGQQVFAGEATRLAYMTDEAAEGRDSFLERREPDWSPFPWHF"
     gene            complement(11246..11617)
                     /locus_tag="PLZ72_07980"
     CDS             complement(11246..11617)
                     /locus_tag="PLZ72_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4499 domain-containing protein"
                     /protein_id="HNA36270.1"
                     /translation="MSVRRQPSLLWRILVTLGIVTLTAAACSDPVWEKVEDTVGDTVP
                     RSTIRSILVGLLAVHSLESLLVWRSARRRGDAGPFRWALATFVWGFPVMGRLRRSRKA
                     EDMALEAVALADEALALTDAA"
     gene            complement(11719..12570)
                     /locus_tag="PLZ72_07985"
     CDS             complement(11719..12570)
                     /locus_tag="PLZ72_07985"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNA36271.1"
                     /translation="MLVTLLGTGSPLPDPERAGPATLVRAGGLDLLFDCGRGVLMRLA
                     AAGSGPTALATVFLTHLHSDHTSDLNDVITTRWVMSMAPSPLTLVGPPGTQAVVDRTL
                     EMLSDDIGYRIAHHDDLTEGPQVHVIEVLDGPVELPALAAAGVAVTAAPTDHRPVTPT
                     VGYRIEHDGRSAVIAGDTVPCEGLDRLVAGAGVYVQTVIREDVIAQIPLARLQDVCDY
                     HSTVTQAAQTAARAGVGTLVLTHMVPAPPPGGAQEWIDVATAHFGGTVLAPDDLTAVE
                     VPPATAG"
     gene            12717..13517
                     /locus_tag="PLZ72_07990"
     CDS             12717..13517
                     /locus_tag="PLZ72_07990"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNA36272.1"
                     /translation="MLAVVALVGLVAACSSDDDSSPTTTATEKTTTTAPKKLTILVSN
                     DDGVGAEGIDALVNALKKMPDTEVIVSAPATNQSGAGGKTTPGELTATPTTMISGTDA
                     TGVAGFPADSVAWALDGGIEQKPQLVVTGVNSGQNIGAFIALSGTVGAARAGAQRGIP
                     ALAVSAGINDAEGYAEAAALAVKWIEEHRAALLDGSMATTPASVTNINVPACSAGTPR
                     GLVEVPASSQPPADLNPVDCSAPAPPAPTDDNGAIAGGWASISDIGMG"
     gene            13883..14728
                     /locus_tag="PLZ72_07995"
     CDS             13883..14728
                     /locus_tag="PLZ72_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36273.1"
                     /translation="MTELGGHDLTGAAALVTGGGSGIGLECARRLVADGCAVTIMGRS
                     EDRLRDGAAALEDVAPGRALVRTSPGDARSEADVADAVRIASEATGGLDFAVASAGRG
                     GLGPIVTTPLEEWTDILETNLTGTFLLFKHAGAAIAASGGGAMVAISSIAAAVTHPFM
                     GPYCVSKAGVDMLVRQTADELGRAGVRVNAVRPGIVATDLVSMVMDDDQVMGSYLENM
                     PVSRSGTVDDVASVVRFLLGGESSWVTGTSLSVDGGHHLRRGPDFEPAARAFFGDDAV
                     EGRVS"
     gene            14725..15936
                     /locus_tag="PLZ72_08000"
     CDS             14725..15936
                     /locus_tag="PLZ72_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36274.1"
                     /translation="MNGPTAGGGATGRPELDLLDGDFYVDDPYSTYAWLRDNEPVYWD
                     AVNELWIVTRYDDVVEVEKHKERFISSDKVKGGYRPNLAADPAIIGLDDPLHSHRRNL
                     VSRRFTPRAVSRWEDHVRSTVSGLFDAAQAHGGPIEIVGELAAPLPAEMIGLLLGFPD
                     EMFPKLMEWSERTIALGGGPRYLNEEGITAAMEFAQASADLYEEKQRCPADDVMTVWT
                     TAEVPGHDFGVDEVISDCLLLLDGGAETTRTVIARGMIELARRPEQWARLRDGVDLSI
                     AVEELIRFVTPVHNMCRVAVEDTVLRGTAIAAGEQLMLMYGSANRDPAHFADPEALDV
                     TRHPNHHLSFGFGTHFCLGSSLARLEIRLFFEELLSRASELRVVEGSVEEMPNAFVYG
                     LRSARMELVAD"
BASE COUNT         2256 a         5577 c         5633 g         2271 t
ORIGIN      
        1 gacctcgccg gctgccaact cgcggctgcg ggcgatcgcg tcggcggcga ccgcagcgac
       61 ggccgcggct cccgcaaccc ccagcaccgt ccggcggtac cgcccgtcga cgacgtcgaa
      121 ccgcttgaag atcggccggg tgacggcggg aaccaccagg ctggccacgc ccatccccag
      181 gctggtggcg gtgtcgcgcc actcgtagtc cccggcggac ggcgcggcgc cggcgtcgcg
      241 ggcccgcttc aggtacagcg cctcggcccc catcgaaccc cagaagaagg ggatcgccgc
      301 gatcgtcaga tccaccatgg gtcgacccta cttctggagc cgacctcgtg gtcggcgaac
      361 ctggcggcgg tgctcagtcg ctgccgagcg ggccgtccgg ggtgagcagg atgaacccgg
      421 agcccagccc ggccgatgcc ccgtgcgcct cggtgccggg gaagagggca tcggtcatga
      481 ccgagccgag cgtgtacggg tcggccccat ccacgtcgac ggtgtagccg aggtcggcca
      541 gcgacgcgat cgacaggcgg ctcagcggtg agggcccgga cgagatccac ccggtcatca
      601 gctcgcttcg gaacgtcgac tcgcgccagt gggcgagcgc ggtgccgggc ccgccgccgg
      661 cttcgatcgg gacgccggtg tcggtcgatg cgctcagggt gttccactcg gccaccgcgc
      721 ccggtccggt gaagcgggga tcggtgggct cggtcgtgtc tctgagcccg gcgtagggcc
      781 acatcgtgcc gatgccgagc acgtggccca tctcgtgcag gacgatccgg tcgaagcgcc
      841 cgttcgccag ccacgtgccg acgtccgcgg cgtcgaagtt catgatgccg gcgcgggcga
      901 gggtgccggt gcggaggcag aaccccgccg acccgagcac gccgccgggg ccgtcgccgg
      961 gaccgaccga gacgtcgatc accacgtcgt cgatcgtgcc gaccggtccc gcgtcgtcgc
     1021 agccgtcgaa cccgctgggg gagtaggggg cgaggtcggc ggtgatcacc tgctcccaac
     1081 gcgtggcggc tgcgttgaac gagtcgatca ccggttggac ctggtccgcg ggcagcgtcg
     1141 ccgcgaaccg cagcgtgatg tcgaacccgt cgatcgggcc gctgcggacc gtggtccagt
     1201 ccagcgtcgt ctccggcgga gcggtcgtgg tcggcgcggt cgtcggctgc accgccggtg
     1261 gagtgtccga gccggccacg ggcggtggtg gcgtgcacgc cgcgaggacg agcaggggag
     1321 cggcgccggc gacgagcaca cggaggaatc gaccgcgacc tctgaccatg tggtctgtgt
     1381 cgacgcgctg cgggcgcgga ttgagcctgt ggccgacaac gggccgtccg gcccatgcgc
     1441 cccggtggcg gtcggctggc tcagccggtc atcgcccgga cgaggtgttc gaactccgcg
     1501 acctcccggg tccgcgccgc ggccaccgcg tcggcccgtc cggcctgcat gagcgccttg
     1561 gtggccagca gcggtccgag cggtagtccg ccgatgtggc ccgccagcgc ccgggcctcg
     1621 tcgagcagcc ggtcgtgggg gaccgcccgc agcgcgatgc cgtcggcgac ggcctgatcg
     1681 gcatcgatcc acggctcggt gaacagcacc tcggcggccc gctgccagcc catcgtcgcc
     1741 ggcatcagga ggctggccga ggcctcggtg gtcacaccga gggagatgaa cgggaccttg
     1801 agccgggcct ggtcggacat gaggacgatg tcgcagtgca acagcatggt caggccgatg
     1861 ccgaccccca cgccgttgac cgcggcgacg atcggtttgg ggaacgactc gagcaccggc
     1921 atgaaccgct ggtagccggg ctcctggccc tcgaacacgg tcgggtcggc catctcgccc
     1981 aggtcctggc cggccgagaa cgcccggccg ttgccggtga ccagcacgca ccggacgtgg
     2041 tcgtcggccg cggcgccctc cagggcggcg gcggccgcgt accagagctc ctggtcgaag
     2101 gcgttgagcg ccccgggccg gttgaacgtg agggttcgga cgccggcgtc gtcgtcgacg
     2161 aggaggaccg gctcggagcg cggggtgtcg gacatgggcc cgatggctag caccgcccgg
     2221 acgtcgccgt caccgtcgtc ctaacgtgac ggccgtgagc accacccccg ccgacctggc
     2281 cgccgcgtcc aacgccgaga gcaccgcggc ggaccgccgc tacgtgttcc actcctggtc
     2341 cgcccaggac ctgatctcac cgatgccgat cgccggtggt gacggcgcct ggttctggga
     2401 caacgagggc aaccggtacc tcgacttcgc ctcccagctg gtgaacctga acctgggcca
     2461 ccagcacccc gacatgatcg ccgcgatcaa ggcccaggcc gacacgctgt gcaccgtcgc
     2521 tccaccgttc gccaacgacg tgcgcaccga ggcggctcgc ctggtcgccg agcacgcgcc
     2581 cggtgcgctc aaccgggtgt tcttcaccaa cggcggagcc gaggccaacg agaacgccgt
     2641 gcggatggcc cgcctccaca ccggacgcca gaaggtgctg gccgcctatc gcagctacca
     2701 cggcgccacc gccaccgcga tcacgctgac cggcgagccg cgccgctggg cgtccgagcc
     2761 gggtgcgccg ggggtggtgc acttcttcgg gccgtacccg taccgctcct cgttcggagc
     2821 ggccaccgac gagcaggagt gcgaacgggc gctcgcgcac ctggccgacg tcgtgcagat
     2881 ggagggcccg cacaccgtcg ccgcgatcgt gctcgagtcc gtcgtcggta caaacggggt
     2941 gctggtgccg cccgacgggt acctggccgg agtgcgcgag ctgtgcgacc gccacgggat
     3001 cctgatgatc gcggacgagg tcatggtcgg cttcggtcgc gtcggtgagt ggttcgccgt
     3061 cgaccgctgg ggggtggagc cggacctgct gaccttcgcc aagggtgtga actccggcta
     3121 cgtccccctc ggtggtgtgc tcatctccga cgacgtggcc gccacgttcg ccgagcgtcc
     3181 gtatcccggc gggctcacct acagcggtca ccccctggcg tgcgcgaccg cggtggcgag
     3241 catcgggatc ttcgaacgcg acggcatcgt cgagcggtcc cgccggctcg gcgacgacgt
     3301 catcgccccg gccctggtcg agatggccga tcggcacccg tcggtgggcg aggtgcgcgg
     3361 catggggtgc ttcttcgcca tcgaactggt ccgcgaccgc gggacccgcg agccgttggt
     3421 cccgttcaac gcggccggcg ccgatgcggc gccgatggtc gaggtgatgt cggcctgcaa
     3481 gtcgggtggg gtctggccct tcgcccactt caaccggctg cacgtcgctc cgccgttggt
     3541 gatctccgag gacgacctgc gttccgggct ggcggtgatc gacgccgcgc tcgacgtctc
     3601 ggaccgtctg gtcggaggct gaggcacccg gtcgcagcgc accggccaac tgccgtgtgc
     3661 cccgacgtga ccgagccgaa ggcgggaccg tccccggtct ggggtagggt ggacgcaccg
     3721 tcggatcacc ggcgggatcc gaacagcgaa ccgctagtcc tcggggtgcc ggaaccgtcg
     3781 gcaccccgtt gcgcaatcag gccccgctca gcggggagaa acgagtcata ccgtggcaac
     3841 gggaaccgtc aagttcttca actccgagaa gggttacggc ttcatcagcc gtgacgacaa
     3901 ctccgaggac atcttcgtcc acttctccca gatcgcgggc agcggctacc gcaacctgga
     3961 agagggacag aaggtcgagt tcgaggtcgg tcccggccgc aagggcgacg aagcccagaa
     4021 cgtccgcgcc gtctgatccg gaccatcgat cgcagctcga cggcgacacc cgacctcggt
     4081 cgggtgtcgc cgtcggcgcg tccgggtccg atccggtgaa cgcggatacg acaccgaccg
     4141 atcgccacct caccccggcg gggtgagtcg ttccgagagg ctgtacgggt agcgtcgcgc
     4201 ccatggccca ggacgacacc ccgacatcgg agcagccggc gaccggcgac gccccggaca
     4261 gcgaactggc ccgtctccgg gccgagaacg aacgcctcca acgggagctg gaagcgaccg
     4321 ggcccgccgg tgctgcagct gggccccgcc accgcggacg ttggttcgcg tcgtgggtcc
     4381 tgatcgtcgt cgggtcgctg ctggtgccgg tgtcggtcac gtcactgtgg ctcaaccgga
     4441 ccatcaccga caccgaccgc tacgtcgaga ccgtcgcccc gctgatccgc gaccaggaca
     4501 tccagcgggc catcgaggcc cgtctcgaga aggtcctcta cgcccaggtc gacatccagg
     4561 ccgaggtcgc caacgtcctg cccgagcgcg ccgccccgct ggccgcgccg atcaccgccg
     4621 ggttgaagaa cctgatcagc gaggtgatcc accgggtggt gaccagcgac aaggtcgccg
     4681 agctgtggga ccgggccaac cggatcgccc aggagcaggt cgaacaggtg ctcaccgtct
     4741 ccaacgggcg caagggcgtg gtgtcggtgg acctcaccga caccctcaag gaggtccaga
     4801 agcagctgac cgacgccggg gtgcccttcg tcgggaacat cgcggtcccc aaggtcgacc
     4861 tcgacgtcat gcagtccgac acgatcgccc aggtccagac cgcgttcggg atcttcgacc
     4921 gcctggccac catcctcccg tggctcaccc tgctcatcct cggagccggg atcctggccg
     4981 cgccggatcg ccgcaagggc ctggtccacg cggcgagcgg ctgggtcatc gggtccctgt
     5041 tgctgctcgt cggtgtcgcg ctgggacgga tgctgtacct gaacgcgctg ccgccggggg
     5101 tgtcgatgcc ggccaacgag accttcttcg acacgatcag ccggttcctg cgtggcggcg
     5161 gtcgcaccgt gttggtgctg ggcctgctca tcctggtcgt cgccctggtc acggggccga
     5221 gcggcccggc ggtcaggttc cgcaccgcgg tgtcccgcct ggtcggggcc gcgggcagcg
     5281 gggtcgatcg gaccggagtc gatctgggcc cggtnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnaaccta gagtgggtcc cagggggtcc caccgtgcac gacgaaccgg
     5461 tcatcgacgc cgacaaccac tactacgagg cgctcgacgc cttcacccgc caccgcgacc
     5521 cggcgtgggc ggaccgcacc gtgcgatggt gcgacatcga cgggcgcaag taccacgtgg
     5581 tcggcggccg ggtgagccgg gcggtcacca acgccacctt cgacccggtc gtccgggccg
     5641 gggcgatgca cgactacttc cggggcaacc ccgacggccg tgacccgctc agcttcctga
     5701 ccgaccgcga accgatccga cccgagtacc gcgaccgcga cgcccgcatc gccaccctcg
     5761 acgagcaggg cctggccgcg tgctggatgt tcccgacgct cggcatgatc tacgaggaac
     5821 tgctcaccgc cgaccccgag gcctgctgcg tcaccttccg ggcgttcaac cgctgggtgc
     5881 aggacgactg gggcttcgcc caccgcgacc gcatcttcgc cgctccgtac atcaccctgg
     5941 ccgatccgga ctgggccgtc gacgagctgc agtgggcgct cgacgccggc gcccggctga
     6001 tcgtcatgcg ccccgccgcg ccgaccacgg tgctcggtcg ggtctcgccg ttcgacccgc
     6061 ggttcgcccg gttctggggc ctgctcgacg agtcgggggt tccgctggtc ctgcacgccg
     6121 gcgacggcgg ggtcagctcc aacggctacg cccccgacgg gttctcggca tcgttctcgg
     6181 gtgggttcac cccgtcgatc aagttcttcg ccatcgaaca ggccatcaag gacttcctgc
     6241 tgtcgatgct gctcgagaac cacctggtgc gctacccgaa cctgcgcatc gtctcggtcg
     6301 agaacggagc cgagttcctg cccgacctgt tccgcaaggc gcgctcgatg gcccacaagg
     6361 tcgccgggtg gttccccgac gaccccgtcg acatgttccg ggcccaggtg tggatcaacc
     6421 cgttctggga ggacgacgtc gtgtcggtcg tcgagcagat gggggcggac cgggtcgtgt
     6481 tcggttccga ctggccacac atcgagggga tgccgcgccc gctcgactac ctgcccgagg
     6541 tcaaggagct gtccgcggcg gaccagcggc tcgtgctgca cgacaacgcc gccggactcc
     6601 tcgcccccgc caccccgtag cacccctatc gttctgtagt gcgagatcca ggcgaacgtg
     6661 tggatctcgc actacagaac gataggggga ctggtgtggc cgatctgacc atccgcaacg
     6721 ggctgctcgt cgacggcacg ggggcaccgg cgcgacctgc cgacgtcgtc gtcgacggcg
     6781 accgcatcgt cggggtgacc gaaccgggtc aggggtccgc cggtcaccgc gagatcgacg
     6841 cggacggccg tctggtcaca cctggcttcg tcgacatcca cacccactac gacgggcagg
     6901 ccacctggga cccagagatg actccgtcgg gctggcacgg cgtcaccacc gtggtcatgg
     6961 gcaactgcgg cgtcggcttc gctcccgcgg acccggcccg tcgggactgg ctgatccagc
     7021 tgatggaggg agtcgaggac atccccggca ccgccctggc cgaggggatg agctggaact
     7081 gggagtcgtt ccccgagtac ctcgacgagc tcgactcgat gccccgggtc atggacgtcg
     7141 cggcccagat cccccacggc gcggtccgcg gctacgtgat gggggagcgg ggcgcggcca
     7201 acgaaccggc ctccgccgag gacatcgcac tcatgtccaa gctggtcgag gaggggctgc
     7261 gggccggtgc ggtcggcttc accaccaccc gcaccgtcct gcaccgggcc aaggacggcg
     7321 agctggccgc cggcaccacc gccgaggtcg aggagctcct cgggctgggc gacgcgctcg
     7381 gcgcggcggg caccggcgtg ttcggtgtgg ccagcgacat gatcgacccc acgcccgagt
     7441 tcgactggat gatcgagatc tcgcggcgaa ccgggcgtcc ggtcaccttc ggatgcctcc
     7501 agaacgacgt ccgacccgaa cagtggcgag agctgctcga ccgggccgcg gacgcgaacg
     7561 ccgccggggc gcgcatcgtc ccgcaggtcg ccgggcgccc ggcgtgcctg ctgttcgggt
     7621 tcgagtcgtc ggtgcacccg ttcatcaccc acagggcgta ccgcgagatc gcccatctgc
     7681 ccctggccga gcgtgtcgcc gagctgcgca cgcccgagcg acgtgaggcg atcctctccg
     7741 aggaggtcac ccggtccggc ttcggcgcct atctcctgtc gagcttccag aagctgttcc
     7801 ccctcggcga cccgcccgac tacgaacctg cccccgaacg cagcgtcgcc gccatcgcgg
     7861 cgcgggaggg tcgacggccc gaagaggtca cctacgacct gatgctcggc cgtgacggca
     7921 ccgagctcct ctacttcccg atgctcggct actcccacgg cgacttcgag gccatgcgcg
     7981 agatgctcga ccgcgacgac acggtcctcg gtctgggcga cggcggggcc cactgcggca
     8041 tcctctgcga cgccagcctc ccgacctaca tgctcaccca ctggtcccgt gaccgcgacc
     8101 gcggcgcccg cttcaccctc gagcaggtcg tggccatgca gacgagccgg accgcagcgc
     8161 tctacggatt cgacgaccgc ggggtggtgg ccccgggact ccgggccgac ctcaacgtca
     8221 tcgacctgga cggcctgcgc ctgtccccgc ccgagatggt ccacgacctg ccggccggag
     8281 gtcggcggct gatccagcgg gccaccgggt tcgccgccac catctgcgcc ggggagccgg
     8341 tccgactcga cgacgagtcc accgggtccc gccccggtcg actggtccgc tcggctcgag
     8401 cttgagcgcg gtcccggtcc tgccaccccg cacggtcggg gggtggtgat ggaggtgctc
     8461 gccgcggtct acggactgga ggacgccgga tcggaggccc gtcgactcga ggctgcgggc
     8521 gtcgacgggg tgttcacctt cgaaggtccc cacgacgtct tcatcccgtt ggcccttgcc
     8581 gcagccgcga ccgacctgtc ggtcatgacc aacgtggcga tcgccttccc ccgcaacgcg
     8641 gtgcacatgg cccacgcggc ctgggacctg cacgtcctgt ccggcggtcg ctgcacgttg
     8701 ggtctgggca cccaggtccg cccgcacatc gagggtcgct tcggggtgga cttcgaccgt
     8761 ccggtcgaac ggatgcgcga cgccgtcgag gcggtgcgat cggtgttccg cacctggcag
     8821 gacggcgtgc cgctcgagca ccggggcccg ttccggaccc acacccggat gtcgccgatg
     8881 ttctcgccgc cgccgcaccc cgccggcccg ccccgcatcg ccgtcggtgc gctcggtccg
     8941 cgcctgaccg cgatggccgc cgaggtggcc gacgcgctgg cggtcatgcc ggtgacctcg
     9001 gaggagttct tcaccgaggt caccctgccg gcggtggatc ggggtcggac cgaccgcccg
     9061 agtcggctgg agcagttgga ggtgctcccg gagctgatcg tgtgctgcgg ccgcgacgca
     9121 gccgagcagg cggtcgccga tgccgggtgc cgggcgctgc tcggcttcta cgtgtcgacc
     9181 cccgcctacc gtccggtctt cgaacgtgcc gggcggggcg acctccaacc ccgcgcccag
     9241 cagctgacgc gggagggccg ctgggacgcg ctcggcgacc tgatcgacga nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacgcgatcg acggcacgtt cgacggggtg
     9421 ctggcggtcc gggtgtcggc cccgccggtc gacggtcgtg ccaacgaggc ggtgcgtcga
     9481 ctgctcgccg acgcgttcgg ggtggcccga tccgaggtca ccatcgtcgg aggccacggg
     9541 tcgcgccgca agcgggtgga gatcgacggt gacgaggccg ggctccgggc tcgtctggcc
     9601 gaactcgctg atcctgcggg ctgagccctg cgtcagctga cggctgggcc gccgcagcgc
     9661 gggtcgcgct cggagcggta gctcgctcga tcctcctgat cagctcgcgg acatcaccgc
     9721 ctgggtctgg gtggtgcgcg ccgccagctt gccgtcgtcg cgggtgaggt cggtctgcac
     9781 gacgatcgtc gtgcgcccga cgtggaccgg agtgctcgtc gcggtcaccg tcccggaggc
     9841 gacggcgcgg atcatgttgg tcttcgactc gatcgtggac gtccacgtgt cgggcgggag
     9901 gttcttgaac gcgcacagcg cgccggctgc atcggcgagc gccatcatgt agccgccgtg
     9961 aaggatcccg cccgtggtgc agtgctcggg ctgccacggt gcactgagca cgacctgctc
    10021 ggccgtgctg ctcagcacgg agaactgcag gagcgcgccg agcggcatca gctcgcgcag
    10081 cgattcggtg gcgacgggat cggtcatggc acagcttcgc gctgctcggc gcgtcgtctc
    10141 gggacggccg gtggcgcctg acagactctg ctcatgtccg atcgacgccc cgagccccgt
    10201 tccgacgaac tcgaccccag cggaccgggc ggttctgccc cggccgtgtc ggagatcttc
    10261 gatcccgagg cgtgggcgcc ggtgcccggc ttcgaggacc tcgacgacat cacctaccac
    10321 cgggcggtcg acgtcggggt ggttcgggtt gcgttcaacc ggcccgaggt ccgcaacgcc
    10381 ttccggccgc gcaccgtcga ccagctctac cgggtgctcg accacgcccg ccagagcccg
    10441 gacgtggggt gcgtcctgct gacaggcaac gggccgtcac ccaaggacgg gggctgggcc
    10501 ttctgctccg gaggggacca gcgcatccgg ggacgcgacg ggtaccgcta caccgtcgag
    10561 gacgagtcgg gcgacccggg actgaccgcc gaggccatcg acccggcccg ctccggtcgg
    10621 ctgcacatcc tcgaggtcca gcggctgatc cgggtgatgc ccaaggtggt catctgcgtc
    10681 gtgcccgggt gggccgccgg cggcggccac agcctccatg tggtcagcga cctcacgctc
    10741 gccagccgcg agcacgcccg cttcaagcag accgacgcgg acgtcgccag cttcgacggt
    10801 gggttcggct ctgcgtacct ggctcgtcag gtcggtcaga agttcgctcg ggagatcttc
    10861 ttcctgggcg acgagtactc agccgaggac gcccaccgca tgggcatggt gaacgccgtg
    10921 gtcgaccacc aggacctgga gcgggtggcg ctcgactggg ggaggaaggt catgggcaag
    10981 tctccgaccg cccagcgcat gctgaagttc agcttgaacc tgatcgacga cggcctggtc
    11041 ggccagcagg tgttcgccgg ggaggcgacc cgcctggcct acatgaccga cgaggcggcc
    11101 gaaggtcgcg actcgttcct ggagcgacgc gaaccggact ggtcgccgtt cccctggcac
    11161 ttctgacccc ggcatccgaa cactggggtc gcgatcgaca cgtcctgtcg attccgcgcc
    11221 ccggaacggg acgagggcgg gaggatcagg ccgcgtcggt caacgcgagg gcctcgtcgg
    11281 ccagggcgac tgcctcgagc gccatgtcct cggccttgcg ggagcggcgc aggcggccca
    11341 tcaccgggaa gccccacacg aaggtggcca gggcccagcg gaacggaccg gcgtcaccgc
    11401 ggcgccgggc ggagcgccac acgaggagcg actccaggga gtgcacggcg agcagtccga
    11461 cgaggatcga tcggatcgtc gaacgcggca cggtgtcgcc gacggtgtcc tcgaccttct
    11521 cccacaccgg gtcgctgcac gcagcggcgg tcagggtcac gatcccgagg gtgacgagga
    11581 tcctccagag caggctgggc tggcggcgga cggacatgtc ggggctcctc gggtctcggc
    11641 cgaatcggtc ggggtcaacc tatcgccgga tcaccgagtt ggggctgatc ggaacgcgaa
    11701 cgccggaacc ggtcgggatc aacccgccgt cgcgggaggc acctcgacgg cggtgaggtc
    11761 gtcgggggcg aggacggtcc cgccgaagtg cgcggtcgcg acgtcgatcc actcctgggc
    11821 tccgcccggt ggcggtgccg gcaccatgtg ggtgagcacg agcgtcccga ctccggccct
    11881 cgccgcggtc tgcgcggcct gggtcaccgt cgagtggtag tcgcacacgt cctggaggcg
    11941 ggccaggggg atctgggcga tcacgtcctc gcggatcacg gtctgcacgt agacgcccgc
    12001 tcccgcaacc aggcggtcga gcccctcaca gggcacggtg tctcccgcga tcaccgcaga
    12061 gcgaccgtcg tgttcgatcc ggtagccgac ggtcggggtc accggacggt ggtcggtcgg
    12121 cgccgcggtc accgcgacgc ccgcagcggc cagcgccggt agctcgaccg gaccgtcgag
    12181 cacctcgatc acgtgcacct gcggtccctc ggtcaggtcg tcgtgatgcg cgatgcggta
    12241 gccgatgtcg tcgctcagca tctcgagggt ccggtcgacg acggcctgcg tcccgggggg
    12301 accgaccagc gtgagcgggg acggcgccat ggacatgacc caccgggtgg tgatcacgtc
    12361 gttgaggtcg gaggtgtggt cggagtgcag gtgggtgagg aacaccgtcg ccaacgccgt
    12421 gggacccgaa cccgccgcgg cgagtcgcat gaggacgccc cgaccgcagt cgaacagcag
    12481 gtccagtcct cccgcccgca ccagcgtggc gggcccggca cgctccgggt cggggagggg
    12541 agaaccggtg ccgagcaacg tgaccaacat ggcggccacg gtagcgtggc ggtccgagcg
    12601 gccagggggg tcgccgccac agggggtgca gtccgatgcc gtcgaccgat ccgtccacga
    12661 gcgtgcgtcg tcgagggtcc gggaggctct tacgccccgc tcgcggcgcc gtcgccgtgc
    12721 tcgccgtagt ggcgctggtc ggactggtgg cagcgtgcag ttcggatgac gactcatcgc
    12781 cgacgacgac cgcgaccgag aagacgacca cgacggcgcc gaagaagctg acgatcctgg
    12841 tcagcaacga cgacggcgtc ggggccgagg ggatcgacgc cctggtcaac gccctgaaga
    12901 agatgcccga caccgaggtg atcgtgtcgg cgccggcgac caaccagagc ggtgccggcg
    12961 gcaagaccac ccccggggag ctgaccgcga cgcccaccac gatgatcagc ggcaccgacg
    13021 ccaccggtgt cgccgggttc cccgccgact ctgtggcgtg ggcgctcgac ggcgggatcg
    13081 agcagaagcc gcagctggtg gtgaccggcg tgaactccgg gcagaacatc ggggcgttca
    13141 tcgccctgtc gggcacggtg ggcgccgccc gtgccggcgc ccagcgtggg atccccgcgc
    13201 tggccgtctc ggccgggatc aacgacgccg agggctacgc cgaggcggcc gcactcgcgg
    13261 tgaagtggat cgaggagcac cgcgccgcgc tgctcgacgg gtcgatggcc acgacgccgg
    13321 cgtcggtgac caacatcaac gtcccggcct gctccgcggg caccccccgg ggactggtcg
    13381 aggtgccggc gtcgtctcag cccccggccg acctgaaccc ggtcgactgc agcgctccgg
    13441 cacctccggc gccgaccgac gacaacgggg ccatcgccgg cgggtgggcc tcgatctccg
    13501 acatcggaat gggctgaccg gattgcacca cccgatgccc accctcggcg ggtggtcgga
    13561 gacggtacgt gggatcttcg ggtcgggccg ggtgaatcgg cgtagcccag accccggaag
    13621 gtgttgaccc tggttcaggt ttgtgttctg gtggatcccg agttcgcgtg gcgatgcgaa
    13681 ccgcaccgtt gtccggtgcg agcaggggcc tgggggcacc ggggtgtccg gaacgacgac
    13741 gtggtcggtc cggttccgac gggagcggtc acgtggtgac cgtcgaccgt cggcacctac
    13801 ggccaccccg agggggagat gatcgacgac ggggcccttt ccgggccccg tcgccgcgtc
    13861 gcagtggcta ccgtgccggt ccatgaccga actcggggga cacgatctga ccggcgccgc
    13921 cgcgctcgtc accggcggcg gcagcggcat cggactggag tgcgctcggc gactggtggc
    13981 cgacggctgc gcggtgacca tcatgggccg cagcgaggac cgactgcgcg acggcgccgc
    14041 cgcgctggaa gacgtcgcac ccggccgggc gctggtgcgc acctcgcccg gcgacgcccg
    14101 ctccgaggcg gacgtggccg acgcggtgcg gatcgcgagc gaggcgaccg gaggactcga
    14161 cttcgccgtc gcctcggccg gtcgcggtgg cctgggaccg atcgtcacca ccccgctcga
    14221 ggagtggacc gacatcctcg agaccaacct gaccggcacg ttcctcctgt tcaagcacgc
    14281 gggcgccgcc atcgccgcga gcggaggcgg tgcgatggtc gcgatctcgt cgatcgccgc
    14341 tgcggtcacc cacccgttca tgggtccgta ctgcgtgtcc aaggccggcg tcgacatgct
    14401 ggtccgccag accgcagatg agctcggccg ggccggggtg cgggtcaacg cggtgcgacc
    14461 gggcatcgtc gccaccgacc tggtctcgat ggtcatggac gacgaccagg tcatgggcag
    14521 ctacctggag aacatgccgg tgtcgcggtc gggcaccgtc gacgacgtcg cctcggtcgt
    14581 gcggttcctg ctcggtgggg agtcgtcgtg ggtgaccggc acgtcgctca gcgtggacgg
    14641 cggccaccac ctgcgccgcg gcccggactt cgaaccggcg gcccgagcgt tcttcggtga
    14701 cgacgcggtc gaggggaggg tgtcgtgaac ggcccgacag ccggcggcgg agcgaccggt
    14761 cgacccgagc tcgacctgct cgacggcgac ttctacgtcg acgatcccta ctcgacctac
    14821 gcgtggctgc gcgacaacga gcccgtctac tgggacgcgg tcaacgagct gtggatcgtc
    14881 acccgctacg acgacgtggt cgaggtcgag aagcacaagg agcgcttcat cagctccgac
    14941 aaggtcaagg gcggctaccg cccgaacctc gccgccgacc cggcgatcat cggcctcgac
    15001 gacccgctcc actcccaccg tcgcaacctg gtgtcgcgtc gcttcacacc ccgggcggtg
    15061 tcgcggtggg aggaccacgt gcgctccacc gtgagcggcc tattcgacgc cgctcaggcc
    15121 cacggcggtc cgatcgagat cgtcggcgag ctggccgctc cgctgccggc cgagatgatc
    15181 ggcctgctgc tcggcttccc cgacgagatg ttccccaagc tcatggagtg gtccgaacgc
    15241 acgatcgccc tcggcggcgg gccgcgctac ctcaacgaag aggggatcac cgcggcgatg
    15301 gagttcgccc aggcgtccgc cgacctctac gaggagaagc agcgctgccc cgcggacgac
    15361 gtcatgacgg tgtggaccac cgccgaggtc cccggccacg acttcggcgt ggacgaagtc
    15421 atctcggact gcctgctgct gctcgacggc ggcgccgaga cgacccgcac cgtcatcgcc
    15481 cgggggatga tcgagctggc ccgccgtccc gagcaatggg cgcggctgcg tgacggggtc
    15541 gacctgtcga tcgccgtcga ggagctgatc cggttcgtga cgccggtcca caacatgtgt
    15601 cgggtggccg tcgaggacac cgtgttgagg ggcaccgcga tcgctgccgg agaacaactc
    15661 atgttgatgt acggctcggc caaccgggac ccggcccact tcgccgaccc ggaggcgctc
    15721 gacgtcaccc gccacccgaa ccaccacctg tccttcgggt tcgggaccca cttctgcctg
    15781 gggtcgtcgc tggcccgact cgagatccgg ctgttcttcg aggagctgct gtcgcgggcg
    15841 tcggagctgc gggtggtgga gggatcagtc gaggagatgc ccaacgcgtt cgtctacggg
    15901 ctgcggtcgg cccggatgga gctcgtcgcc gactgag
//
LOCUS       DAOQOD010000064        15927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1305_length_15927_cov_11.228516, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000064 DAOQOD010000000
VERSION     DAOQOD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15927
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1305_length_15927_cov_11.228516"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLZ72_08005"
     CDS             <1..440
                     /locus_tag="PLZ72_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA36275.1"
                     /translation="SVGSGSLMVPLLLMLYPPLTSNQLVGTDLVQAVPLVAAATLGHA
                     AFGEVQLDLTAALLVGALPGVWIGAHISSRAPDHLIRPLLAVVLIVSGLKMLGVGSLA
                     VGVIGLVLLAGAVAVILRSSAAAAQRSGSSRPHRRAADRARAS"
     gene            complement(380..1111)
                     /locus_tag="PLZ72_08010"
     CDS             complement(380..1111)
                     /locus_tag="PLZ72_08010"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNA36276.1"
                     /translation="MGSIRIVGAGRAGGSFALALGGVGWVPEVMLRGVPLEAAADGVD
                     VVLLCVPDGAVAEVAASIRPNPSCVVVHCAGSLTLDVLDPHPVRASVHPLVSLPSPAV
                     GAQRLAGAWFGVAADPPGRDRVDELVGALDGRVVEVADRDRATYHAAAAMASNHLVAL
                     LGQVERTAASVGVPLDAYLDLARGSLANVAELGPAAALTGPVSRGDWATVAAHLAALP
                     AEERPAYEALARSAARLCGRELPDL"
     gene            complement(1129..1593)
                     /gene="folK"
                     /locus_tag="PLZ72_08015"
     CDS             complement(1129..1593)
                     /gene="folK"
                     /locus_tag="PLZ72_08015"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNA36277.1"
                     /translation="MSTPAVRTFLSLGSNLGDRRQHLSQAIESLPDVVAVSPVYETEP
                     VGGPGGQGPYLNLVVELHTPIAPRALLSICHRIEANAQRVRGERWGPRTLDVDILWMD
                     GVTMADEALTLPHPRWKDRRFVLAPLRDLAPDLVTERELQLSDGVVTRVEDL"
     gene            complement(1590..2009)
                     /gene="folB"
                     /locus_tag="PLZ72_08020"
     CDS             complement(1590..2009)
                     /gene="folB"
                     /locus_tag="PLZ72_08020"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNA36278.1"
                     /translation="MADNPVSDTIELRGLRVVGVVGVLAEERERAQPLEIDLDVDVDL
                     TAAGVSDDLSDSVDYGELCDVVVATVAGPGAERPELLERLAASIADAVLDRDDRIGAV
                     TVSVRKLRPPVPHDLSTSGVRIRRQRRSRTSTIGFSR"
     gene            complement(2029..2544)
                     /locus_tag="PLZ72_08025"
     CDS             complement(2029..2544)
                     /locus_tag="PLZ72_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36279.1"
                     /translation="MAAKGKWAQGIKPRHFHWIIQDQLAVCERPGGFGANHRKVRRQE
                     EIIWIREQGFDLVVSVSPAPHNLHNYDEMGVTWLHWPLPSAEESSRYLERTYGGLRQL
                     LGSRKQVIVHADELSDRLVGLMAGYLVWSGMVPEPPQAINIIERLCGRQLDADGRTVV
                     NDAARLAAAGD"
     gene            complement(2585..3487)
                     /gene="folP"
                     /locus_tag="PLZ72_08030"
     CDS             complement(2585..3487)
                     /gene="folP"
                     /locus_tag="PLZ72_08030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNA36280.1"
                     /translation="MSGGRAPDPVDRPLVMGILNVTPDSFSDGGVHDDVERARARTLE
                     MIAEGVDVVDVGGESTRPGAAPTDPGAEQARVLPVIEAILEPCRAAGVRLSIDTRHES
                     TARAALAAGATLLNDVSASLAGVAAQAGAGWIAMHMVGDPRTMQVSPRYDDVVAEVFG
                     FLRERAAAATAMGVSEVWVDPGIGFAKTTAHNLALLAHLDDLVADGTPVVVGTSRKRM
                     LGVLAARSDAGLAPFVGDGDGGGGTAEPPPVDVSDRLAGSLSTATWAMILGARMVRVH
                     DVGATARVLGVVAAARAAQRQSGV"
     gene            complement(3484..4116)
                     /gene="folE"
                     /locus_tag="PLZ72_08035"
     CDS             complement(3484..4116)
                     /gene="folE"
                     /locus_tag="PLZ72_08035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNA36281.1"
                     /translation="MTFDDLNALDPEPETPLASAIDRDRIARAVREILEAIGEDPDRE
                     GLRDTPSRVARMYAETCSGLHEDATQHLKVTFDADHDEMVMVRDIPLYSMCEHHLVPF
                     YGFAHVGYIPNTDGRVTGLSKLARLVDGFARRPQVQERLTTQVAEAIEATLQPRGVLV
                     VVEAEHLCMAMRGVRKPGASTVTSAVRGVFRDDVSARMEAMQFVESGRRR"
     gene            complement(4113..5981)
                     /gene="ftsH"
                     /locus_tag="PLZ72_08040"
     CDS             complement(4113..5981)
                     /gene="ftsH"
                     /locus_tag="PLZ72_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNA36282.1"
                     /translation="MTEPDPSPPPAPARPPRSPRRGRFHLESPWVRVAVAVAVGFVVV
                     LIWTTVLRPDPPQELRLDQFQDRLRDDRVRTATIVNSSSSVEGELTDGTEYRTVFPEA
                     YGETLTRQLLARGVEAQADNSDGSLWSDIVFGLLPTFLIVGVFVWFVLNMQRGGKALT
                     FGRARTAAGDRAGDVTFADVAGADEAVTELREVVEYLRDPERFARLGARIPKGVLLVG
                     PPGTGKTLLARAVAGEAGVPFLSLSGSDFVEMFVGVGASRVRDLFRQAVDKAPAIVFV
                     DEIDAVGRHRGAGVGGGHDEREQTLNQLLVEMDGFAQTDGVVLIAATNRPDILDPALL
                     RPGRFDRQVVVDTPDIAGRTAVLEVHLRGKPVADDVDVAVLARRTPGFSGADLANLVN
                     EGALLAARRGGDRIAAVDLTAAVERVLAGPERSRILSEDERRVVAVHESGHAIVSHVL
                     PHADGVHKVSIVSRGAALGYTVLLPEEDRHLHSRAQLSDQLAVALAGRAAEEAVFDQL
                     TTGAIDDIQRATRLARAMVTEYGMSDKLGPRRLVSGDGEPFLGLDQGRPADHGAEVAA
                     RVDEEVSRLLDEAHERARRILEDHRPGLDRLASTLLERETVADAELADLLAAVGDP"
     gene            6125..8533
                     /locus_tag="PLZ72_08045"
     CDS             6125..8533
                     /locus_tag="PLZ72_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNA36283.1"
                     /translation="MADVTSPSSSASTAADVAVDHGERRMVRSPTSLLRLIVGVTVTA
                     VGVVITWRFANTMAALNRDWEQLTHLLPGWIRAIPPLVVGLTLILVPVVVNVQLLRYR
                     RLRLFGVVNLAALAAFAISEVAVDALTRQPPGLFPSAYSAAKGSVNDPLLAAFVAAFV
                     VGIPYLPAATRRLAGWVIGLSLIATLGFAEVPAVAWVTDVGIGITCGAAVALLFGTPD
                     TAPDRRELIAGLARSGIDMADIRPAAVDARGSTPWLGTTRSGRGVFVKVLNQDNRSAD
                     LMFRGFRALFLRNTGDERPMSSLRRAVEHEALLSLRAAAAGVPTPELLTVSEIGNDAM
                     LLAFEAIEGDSLDRVGSDAVTDDLLDSIWAQVVTLQDHGIAHRDLRLANVFVAADGEL
                     YVIDFGFAELAASPLLLATDLAELIASTAPVVGVERAVDAAERAVGVDGLARAFARLQ
                     PYALGGATRAALKEADLLDPLRAEVERRARIPEPDYHGTVPASGRAAVLPILLGLATT
                     AALIVGVPGSSTGPAPIAAWGQVSWVVVATVIGLAAATTASIGSLRDGITVRHVLLSR
                     LAAGFADTLAPWHTANIATRVGVLRSHGIETAAALGSVGLAVVTRIVTQLATLYLAVR
                     ISGRDGSIDVVADADVGWVVAAGAGVVVGALTALLPGPRRSITRAVTPALRAWIAGLR
                     SLTGRPVRLAQLLVGSLFVPLAMVGAMVAADRAVGGTLDPPSLALVTLGVAFLAAFLP
                     FPGGAGVTEAGLVGGLVLLGERAELAVSAVVLFRVATFWLPVAAGAVSMRWLRRNAQL
                     ASGS"
     gene            complement(8512..9075)
                     /gene="hpt"
                     /locus_tag="PLZ72_08050"
     CDS             complement(8512..9075)
                     /gene="hpt"
                     /locus_tag="PLZ72_08050"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNA36284.1"
                     /translation="MDVPGPTSFDDDPAIGRVLLTEEQIATRIAELGAQLTGDYAGSA
                     PLLVCVLRGAYVFLTDLARCIDLPVEIDFIAVSSYGATTRTSGVVRLVKDLEVDLTDR
                     DVILVEDIVDTGLTLRYLRRSLEARGPSSLEVCALLARESADLEGLGVRYVGFTIPDD
                     FVIGYGLDVAERYRNLRYLAIYDPDAS"
     gene            complement(9087..10049)
                     /gene="tilS"
                     /locus_tag="PLZ72_08055"
     CDS             complement(9087..10049)
                     /gene="tilS"
                     /locus_tag="PLZ72_08055"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNA36285.1"
                     /translation="MRRPEAPVGATIDALLRRCSFPPVGSSVTCAVSGGADSSALLVL
                     AVAAGLDVTAVHVDHGLRPGSADEALVVEDLAHRLGTRFRAETAHVAPGPDLEARART
                     ARHAAVGPDALFGHTADDQAETVLLRLLRGTGPAGLAAMTARQHPILALRRSETHDLC
                     RQLRIDVVVDPTNDSWDHRRNRVRHRVLPLLDEVAERDVVPLLCRLAGLAADQSALVD
                     SLAADLDPTDAAVLVAAPEPLASSAVRRWWRDVTGSGLGPDAAALQRILDVAAGRAIG
                     CDVVGGWRVARSAGRLRLVAPAGGAHRRPDGSGNPTGGAPVPGH"
     gene            complement(10036..11100)
                     /locus_tag="PLZ72_08060"
     CDS             complement(10036..11100)
                     /locus_tag="PLZ72_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNA36286.1"
                     /translation="MSSSIDWALAERVAARIAGEEPFAASYHADRMADELQELTAVAQ
                     PRVESETGFTSQAGAARARVVGRLDWVRANLASYRRMLRPVLGRLEELDGPAAAVSGK
                     VAGAELGALLGWMSTRVLGQYDLLVLEEEDPDDQDLVYYVGPNILGLEKRQSFPPGEF
                     RLWVALHEVTHRTQFTGVPWLRPYFLGLVEQLVDAADPDPTRLFTAVGRVAEGIRTRT
                     NPLDDGGIAALFATPEQRLTMDKVGGLMSLLEGHGDVVMDRAATDLVPSAPRFARVLR
                     ARRSEMNLATRLLQRVIGLEAKLAQYQQGEAFIAAVEDAGGPALLNRAFERSELLPSL
                     AEIRDPDTWIRRVGAAVATA"
     gene            complement(11172..11324)
                     /locus_tag="PLZ72_08065"
     CDS             complement(11172..11324)
                     /locus_tag="PLZ72_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36287.1"
                     /translation="MQVAALLADSPVWHYWLAVPIALGAIFVVIAVVLGYFFKVTSTR
                     YPSDEG"
     gene            complement(11335..11712)
                     /gene="rsrA"
                     /locus_tag="PLZ72_08070"
     CDS             complement(11335..11712)
                     /gene="rsrA"
                     /locus_tag="PLZ72_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HNA36288.1"
                     /translation="MSPTPSASPTPGDRADRPDPSGQVDHPGHDHSVASDCDEAIAEL
                     YAFLDGELDDRVMLQVETHLRRCSPCLEAFDFEADLRRVIAAKCTEQVTPEVRARFCG
                     LLHELSSGGTDGVSGVTAPSDGS"
     gene            complement(11744..12328)
                     /locus_tag="PLZ72_08075"
     CDS             complement(11744..12328)
                     /locus_tag="PLZ72_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA36289.1"
                     /translation="MADQSTFADQVEPFVDQLYAAALRMTRNPADAEDLLQETFVRAF
                     RGFPNFTDGTNLRAWLYRILTNTFINSYRAKQRRPEQTELDEVEDLYLYRRLGGLEAA
                     QLGRSAEDELMDMFTEGDVRQALDDLPESFRMAVLLADVEGFSYKEIAEIMDVPIGTV
                     MSRLHRGRKALQRSLHQFAVERGLADRDPVVIEP"
     gene            complement(12422..12571)
                     /locus_tag="PLZ72_08080"
     CDS             complement(12422..12571)
                     /locus_tag="PLZ72_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36290.1"
                     /translation="MSSTTGCRHCRGLCAGTADRCHDCGRWLRSQDYDAGGSPQRSGV
                     GRHSG"
     gene            complement(12710..13624)
                     /locus_tag="PLZ72_08085"
     CDS             complement(12710..13624)
                     /locus_tag="PLZ72_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36291.1"
                     /translation="MQGRNRAHRRSAFSLLVCLVAVMAAVLVGTTNAGAAGDDVDAPD
                     TAATPTPAVEVPDGPVEVPDGQGAEAPPAEQPAEEADGIITITQAPLPFDGQCNPLAQ
                     AQLTYTTTSDADVFRIVITSATKPCNPIAAAAVAYAMPKGASPAGGQWPQTLVERQDF
                     TIAEASTTTITFAKECDAVQFDVITGESPQTISPTGPWHGPLLFVGDTKTSEQYFPPM
                     EKCGTAPTTVPAPPTTATVQAATTVPQTATNVVTGATVADTTATQGENAAALALTGAS
                     SARLALIGAGLLVAGGALFAWSRRSREQ"
     gene            13843..14613
                     /locus_tag="PLZ72_08090"
     CDS             13843..14613
                     /locus_tag="PLZ72_08090"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNA36292.1"
                     /translation="MTRDELLRTFDLTDRVAVVTGGSRGIGRAVAEGFAAVGARVVVA
                     SRKADACDEAATAIRRAGGTALAVPTHMGDLDAVARLVRTTVEEFGGIDVVVNNAANP
                     LALPIGSITPDALAKSHDVNLRGPLFLVQEALEHLRASDHAAIVNVLTAGIFTRGEYV
                     SLYVAAKSALAAFTRAMAAELAGDGIRANGLAPGTVRTDMVLNTPEEFQALAVDAQLI
                     KRMAEPEEMVPAALFLASDASSFMTGQTLVVDGGMTSH"
     gene            complement(14668..14847)
                     /locus_tag="PLZ72_08095"
     CDS             complement(14668..14847)
                     /locus_tag="PLZ72_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36293.1"
                     /translation="MTNDAEHHGLERHPHGGQEPGDTDVQAERQEEGVEAEIEEEIVE
                     AHRRELARQEEGSTG"
     gene            complement(14857..>15927)
                     /locus_tag="PLZ72_08100"
     CDS             complement(14857..>15927)
                     /locus_tag="PLZ72_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36294.1"
                     /translation="RADAARRCAARLRAGGVQLEGYTMLEAADDIRDLVRALDLGRVS
                     AAAGGYTTTAAAAWARANPGSVRSLLLTNPTPPGESVMADPARSLAASFARLRHLCDT
                     DDRCRTAYPDLATAYLQRFGQLDADPVLVETTALGGLGPFRVLLDGRRYAAGLESAMR
                     QSSRLGLVPSAVRGASDELTAAAGIDEDVSFYAGSTFGGAFLSATCSYDERLNRTAEI
                     SDSAMRQFAGANEPTFGRLCEAWGVPSVFDELSPPLTIDVPVLIAVGALSASGVDDWA
                     DAMAASLPRATVVDVPTMSEDLAFSPPPCLRELRARFLREPDAQLAADACRKDPPTID
                     FVTPDWSPAPPRSVVLARTGNG"
BASE COUNT         2183 a         5782 c         5755 g         2207 t
ORIGIN      
        1 cgtcggtcgg atccggctcg ctcatggttc cgctgctgct gatgctctac ccgccgttga
       61 cctccaacca gctcgtcggc accgacctgg tccaggccgt tccgctcgtc gccgcggcga
      121 cgctcggcca cgcggcgttc ggagaggtgc agctggacct gaccgcggcg ctcctggtgg
      181 gtgcgctccc gggcgtgtgg atcggggcgc acatcagctc ccgagccccc gaccacctga
      241 tccgacctct gctggcggtc gtgctgatcg tgtcgggcct gaagatgctc ggggtcggct
      301 ctctcgcggt gggcgtgatc ggactggtgc tcctggccgg cgccgtggcg gtgatactcc
      361 gctcgagcgc cgcggccgct cagaggtcgg ggagctcccg gccgcacaga cgggccgcgg
      421 accgagccag ggcctcgtag gccgggcgct cctccgccgg cagggctgcc aggtgcgccg
      481 ccaccgtcgc ccagtcgccg cgggagaccg ggccggtcag ggcggccgcc gggcccagct
      541 cggcgacgtt ggccaggctg ccgcgcgcca ggtccaggta cgcgtcgagc ggcacaccga
      601 ccgaggccgc ggtgcgttcg acctgaccga gcagcgccac caggtggttc gacgccatcg
      661 ccgccgcggc gtggtaggtc gcgcgatcgc gatcggcgac ctcgaccacc cgcccgtcga
      721 gtgcaccgac cagctcgtcg acccgatccc ggcctggtgg atcggccgcg acaccgaacc
      781 acgccccggc cagccgttgc gcccccaccg ctggcgacgg caacgacacc agcgggtgga
      841 ccgacgcccg gaccgggtgc gggtcgagga cgtcgagggt gagcgacccc gcgcagtgca
      901 cgaccacgca gctcgggttc ggtcggatcg acgccgccac ctcggcgacg gcaccgtcgg
      961 gtacgcacag cagcacgacg tcgactccgt cggccgcggc ctccaggggg acgccacgca
     1021 acatcacctc gggcacccag ccgaccccgc ccagggccag ggcgaaggaa cctccggccc
     1081 gacccgcgcc gacgatacgg atggagccca cggcccggcg tcccggcctc agaggtcctc
     1141 gacccgggtg acgacaccgt cggagagctg cagctccctc tcggtgacca ggtccggggc
     1201 caggtcccgc aacggtgcca gcacgaagcg gcgatccttc cagcgggggt ggggcagggt
     1261 gagcgcctcg tcggccatcg tgacaccgtc catccacagg atgtcgacgt cgagggtgcg
     1321 cggcccccag cgttcgcccc gcacccgctg ggcgttggcc tcgatgcgat ggcagatcga
     1381 cagcagcgcc cggggggcga tcggcgtgtg cagctcaacc accaggttca ggtagggccc
     1441 ctgcccgccc ggcccgccga ccggttcggt ctcgtagacc ggcgacaccg cgacgacgtc
     1501 ggggagcgac tcgatcgcct ggctcaggtg ctggcgacgg tcgcccaggt tggagccgag
     1561 ggagaggaag gtgcggaccg caggagtgct caccgggaga acccgatggt cgaggtcctc
     1621 gaccggcgct ggcgacggat gcgcactccg ctggtggaca ggtcgtgcgg caccggcggt
     1681 cgcagcttgc gcacgctcac cgtcaccgcg ccgatccggt cgtcacgatc gagcaccgcg
     1741 tcggcgatcg atgccgccag gcgctccagc agctccggcc gttcggctcc gggacccgcc
     1801 acggtcgcca cgaccacgtc gcacagctcg ccgtagtcga cgctgtcgga gaggtcgtcg
     1861 gagacccccg ccgcggtcag gtcgacatcg acgtcgaggt cgatctcgag cggttgggcc
     1921 cgttcgcgct cctcggccag cacgccgacg acgcccacga cccgcaggcc gcgcagctcg
     1981 atcgtgtcgc tcacggggtt gtcggccacg acgacgcgtc cggtccgctc agtcgcccgc
     2041 ggcggccaac cgtgcggcgt cgttgaccac ggtgcggccg tcggcgtcga gctgacggcc
     2101 gcagaggcgc tcgatgatgt tgatggcctg cggcggctcg ggaaccatcc ccgaccacac
     2161 caggtatccc gccatcaggc cgaccagtcg gtccgacaac tcgtcggcgt gcacgatgac
     2221 ctgcttgcgg gagccgagca gctgtcgcag gccgccgtag gtgcgctcca ggtagcggct
     2281 ggactcctcg gcgctcggca gcggccagtg caaccaggtg acgcccatct cgtcgtagtt
     2341 gtgcaggttg tggggagccg gcgacaccga cacgaccagg tcgaagccct gctcgcggat
     2401 ccagatgatc tcctcctggc gccgcacctt gcgatggttg gccccgaacc cgccggggcg
     2461 ctcgcacacc gccagctggt cctggatgat ccagtggaag tgacggggct tgatcccctg
     2521 cgcccacttg cccttggctg ccaccggcac ctcctccgat cgatcgtcac cgtcccgggc
     2581 gggctcacac cccggactgc cgctgtgcgg cacgggccgc agcgacgacg ccgaggacgc
     2641 gcgcggtcgc cccgacatca tggacccgca ccatgcgggc tccgaggatc atggcccacg
     2701 tcgccgtgct cagcgaaccg gcgaggcggt ccgacacgtc caccgggggc ggctccgccg
     2761 tccctccgcc gccgtcaccg tcgcccacga acggcgccaa cccggcatcg gagcgggcgg
     2821 cgagcacccc gagcatgcgc ttgcggctcg tcccgaccac caccggtgtg ccatcggcca
     2881 ccaggtcgtc gaggtgggcg agcagcgcca ggttgtgggc ggtcgtcttg gcgaagccga
     2941 tccccggatc gacccacacc tcgctcacac ccatcgccgt cgccgccgcc gcccgctcgc
     3001 gcaggaagcc gaacacctcg gcgacgacgt cgtcgtaccg gggcgagacc tgcatggttc
     3061 gggggtcgcc caccatgtgc atggcgatcc aaccggcacc ggcctgcgct gcgacccctg
     3121 ccagcgacgc gctgacgtcg ttgagcagcg tcgcgcccgc ggcgagagcc gcccgggcgg
     3181 tcgactcgtg acgggtgtcg atcgacagtc tcacgcccgc ggcccggcag ggttcgagga
     3241 tcgcctcgat gaccggcagg acgcgggcct gctcggcgcc cggatcggtc ggcgcggccc
     3301 cggggcgggt cgactcgccg ccgacgtcga cgacgtcgac gccctcggcg atcatctcga
     3361 gggtgcgggc ccgggcccgc tcgacgtcgt cgtgcacgcc gccgtcggag aacgagtcgg
     3421 gcgtgacgtt gaggatcccc atcaccagcg gacggtccac cggatcgggg gcacgaccac
     3481 cgctcaacgg cggcgaccgg actcgacgaa ctgcatggcc tccatccggg ccgacacgtc
     3541 gtcgcggaac accccgcgga ccgccgaggt caccgtcgac gcgcccggct tgcgcacgcc
     3601 gcgcatggcc atgcacaggt gctcggcctc gaccacgacc agcacgcccc gcggctgcag
     3661 cgtcgcctcg atcgcctcgg ccacctgggt ggtcagccgc tcctggacct gggggcggcg
     3721 ggcgaacccg tcgaccaggc gggccagctt ggacaagccg gtgacccgac cgtcggtgtt
     3781 ggggatgtag ccgacgtggg cgaacccgta gaacggcacc aggtggtgct cgcacatgga
     3841 gtacagcggt atgtcgcgca ccatgaccat ctcgtcgtgg tccgcgtcga aggtgacctt
     3901 caggtgctgc gtggcgtcct cgtgcaggcc cgagcacgtc tcggcgtaca tgcgagccac
     3961 ccgcgacggc gtgtcgcgga ggccttcacg gtcggggtcc tcgccgatgg cctcgaggat
     4021 ctcccgcacg gcccgggcga tgcgatcgcg gtcgatcgcg gaggccagcg gcgtctcggg
     4081 ttcggggtcg agcgcgttca ggtcgtcgaa ggtcacgggt ctcccacggc ggccaggagg
     4141 tccgccagct cggcatcggc caccgtctct cgttccagca gggtggaggc cagacggtcc
     4201 agcccggggc ggtggtcctc gaggatacga cgcgcccgct cgtgcgcttc gtcgagcagc
     4261 cgggagacct cctcgtcgac acgggcggcg acctcggcac cgtgatcggc cgggcgaccc
     4321 tggtcgaggc ccaggaacgg ctcgccgtcg ccggacacga gacggcgggg tccgagcttg
     4381 tcgctcatgc cgtactcggt gaccatcgcc cgggccagtc gggtggcgcg ctggatgtcg
     4441 tcgatcgccc cggtggtcag ctggtcgaag accgcctctt cggccgcccg gccggccagc
     4501 gccaccgcca gctggtccga cagctgggcc cgactgtgga ggtggcggtc ctcctccggc
     4561 agcagcaccg tgtagcccag ggccgccccc cgcgacacga tcgacacctt gtggaccccg
     4621 tccgcgtggg gcagcacgtg gctgacgatg gcgtggcccg actcgtgcac cgccaccacc
     4681 cggcgctcgt cctcgctgag gatccgactc ctctccggac cggcgagcac ccgctcgacc
     4741 gcggcggtga ggtcgaccgc ggcgatgcgg tcgccgccac gccgggctgc gagcagcgcc
     4801 ccctcgttga ccaggttggc caggtcggcc ccggagaagc ccggggtgcg ccgggcgagg
     4861 acggcgacat ccacgtcatc cgcgaccggc ttgccacgga ggtggacctc gagcaccgcg
     4921 gtcctcccgg cgatgtcggg agtgtcgacg accacctggc ggtcgaaccg gcccgggcgc
     4981 agcagggccg ggtcgaggat gtcgggccgg ttggtcgccg cgatcagaac caccccgtcg
     5041 gtctgggcga acccgtccat ctcgacgagg agctggttga gggtctgctc ccgttcgtcg
     5101 tgtccgccgc cgacaccggc gccgcggtgg cggccgacgg cgtcgatctc gtcgacgaag
     5161 acgatcgcgg gcgccttgtc gaccgcctgg cggaacaggt cgcgcacccg actggcgccg
     5221 accccgacga acatctcgac gaagtccgag cccgacaggc tgaggaacgg gacgcccgct
     5281 tcgccggcca cggcccgggc gagcaacgtc ttgcccgtgc ccggtgggcc gacgagcagg
     5341 acgcccttgg ggatgcgggc gcccaggcgg gcgaagcgct ccgggtcccg caggtactcg
     5401 accacctcac gcagctcggt gacggcctca tcggccccgg cgacgtccgc gaacgtgacg
     5461 tcgccggccc ggtcgccggc cgcggtgcgg gcccgaccga aggtcagcgc cttgccgccg
     5521 cgctgcatgt tgaggacgaa ccacacgaac acgccgacga tcaggaacgt gggcagcagc
     5581 ccgaacacga tgtcggacca gaggctcccg tcggagttgt ccgcctgggc ctcgaccccg
     5641 cgggcgagca gctggcgggt cagcgtctcg ccgtaggcct cggggaacac ggtgcggtac
     5701 tcggtcccgt cggtgagctc gccctcgacg ctcgacgacg agttcacgat cgtggcggtc
     5761 cgcacccgat cgtcgcgcag gcggtcctgg aactggtcga gacgcagctc ctggggcggg
     5821 tcgggccgca gcaccgtcgt ccagatgagc acgacgacga acccgacagc gacggcgacc
     5881 gcgacgcgga cccagggcga ctcgaggtgg aaccgcccgc gccgcggcga gcggggcggg
     5941 cgcgccggag cgggcggagg ggaggggtcg ggctcggtca ccggcgctca ggctaccgag
     6001 gggcccctga cgctcccacc ggatcgggac gcgccgaccg actcggagcc ccatccccgg
     6061 gggaccgctc gctaccatcc gggtcggcgc cgagcccgac cggacggcgc caccgtcccg
     6121 cacgatggcc gacgtgacct cgccctcctc ctccgcgtcg accgcggccg acgtcgccgt
     6181 cgaccacggc gaacgccgga tggtgcggtc gcccacgtcg ctgctgcggc tcatcgtggg
     6241 cgtgaccgtg accgcggtcg gggtcgtgat cacctggcgg ttcgccaaca ccatggccgc
     6301 cctcaaccgc gactgggagc agctgaccca cctgctcccg gggtggatcc gggccatccc
     6361 gccgctggtc gtgggcctga ccctgatcct cgtgccggtg gtggtcaacg tccagctgct
     6421 gcggtaccgg cggttgcggc tgttcggcgt ggtcaacctg gccgccctgg ccgcgttcgc
     6481 catcagcgag gtggccgtcg acgccctgac ccgccagcca cccggcctgt tccccagcgc
     6541 ctactccgcg gccaagggat cggtgaacga cccgctgctg gccgccttcg tcgccgcgtt
     6601 cgtcgtcggg atcccgtacc tgccggccgc cacccgacgc ctcgccggtt gggtgatcgg
     6661 cctcagcctc atcgccacgc tcggcttcgc cgaggtgccg gccgtggcct gggtgaccga
     6721 cgtgggaatc gggatcacct gcggcgccgc cgtcgccctg ctgttcggaa cccccgacac
     6781 cgccccggac cgccgggagc tcatcgccgg actggctcgc tccggcatcg acatggccga
     6841 catccggccc gccgcggtcg acgcccgcgg ctccacgccg tggctgggga cgaccagatc
     6901 gggtcgcggg gtgttcgtca aggtcctcaa ccaggacaac cgctctgcgg acctcatgtt
     6961 ccgcgggttc cgcgcgctgt tcctgcgcaa caccggtgac gagcgaccga tgtcatcgct
     7021 gcggcgagcg gtcgagcacg aggccctgct ctcgctgcgt gccgccgcgg ccggggtgcc
     7081 gaccccggag ctgctcaccg tctcggagat cggcaacgac gcgatgttgc tggcgttcga
     7141 ggcgatcgag ggcgactcgc tcgaccgcgt cgggagcgac gccgtcaccg acgacctgct
     7201 cgactcgatc tgggcgcagg tcgtgaccct gcaggaccac ggcatcgccc accgggacct
     7261 ccggctggcc aacgtcttcg tcgccgccga cggcgagctc tacgtgatcg acttcggctt
     7321 cgccgagttg gccgcgtcac cgctgctgct cgccaccgac ctggccgagc tcatcgcgtc
     7381 gaccgcaccg gtcgtcggcg tcgagcgggc cgtcgacgcc gcggagcgcg ccgtcggtgt
     7441 cgacggcctg gcccgtgcgt tcgcccggct ccagccctac gcactcgggg gcgcgacccg
     7501 ggccgctctc aaagaggccg acctgctcga cccgctgcgg gccgaggtcg aacgccgggc
     7561 ccggatcccc gaacccgact accacgggac ggttcccgcg tcgggtcggg cggccgtcct
     7621 ccccatcctg ctgggcctcg ccacgaccgc cgccctgatc gtcggtgtgc cgggatcgtc
     7681 caccggcccg gcaccgatcg cggcatgggg tcaggtctcc tgggtcgtgg tcgccaccgt
     7741 gatcgggctg gccgccgcca ccaccgcaag catcgggtcc ctgcgcgacg ggatcacggt
     7801 tcggcacgtc ctgctgtcgc ggttggccgc cggcttcgcg gacaccctgg ctccctggca
     7861 cacggcgaac atcgccaccc gcgtcggcgt cctgcgatcg cacgggatcg agacggccgc
     7921 ggccctgggt tcggtcggac tcgcggtggt gacccgcatc gtgacccagc tcgccaccct
     7981 gtacctggca gtccgcatct cgggtcgtga cggatcgatc gatgtcgtcg ccgacgccga
     8041 cgtgggttgg gtcgtggccg ccggtgccgg cgtcgtcgtc ggtgcgctca cagcgctgct
     8101 gccgggtccg cgccggtcga tcacccgggc ggtgacaccg gcgctgcggg cgtggatcgc
     8161 cggactgcgc agcctgaccg gccgccccgt ccgactggct cagctcctcg tcggctcgtt
     8221 gttcgtgccg ctggccatgg tcggggcgat ggtggcggcg gaccgggcgg tgggcggcac
     8281 cctggacccg ccgtcgctcg cgctcgtcac cctcggcgtg gcgttcctcg cggcgttcct
     8341 tccgttcccg ggcggtgccg gcgtcaccga agccggcctg gtcggaggac tcgtcctgct
     8401 cggcgaacgg gccgagctcg cggtcagcgc cgtcgtgctg ttccgggtgg cgacgttctg
     8461 gctgccggtc gccgccggag cggtgtcgat gcgctggctg cgccggaacg ctcagctggc
     8521 gtcggggtcg tagatcgcca ggtaccggag gttgcggtag cgctccgcca cgtcgagccc
     8581 gtagccgatc acgaagtcgt cggggatggt gaagccgacg tagcggacac cgagaccctc
     8641 caggtcggcc gactctcggg ccagcagcgc gcacacctcg aggctggagg gtcctcgggc
     8701 ctcgaggctc cggcgcaggt agcgcagcgt gaggccggtg tcgacgatgt cctcgacaag
     8761 gatgacgtcc cggtcggtca ggtcgacctc gaggtccttc accaatcgga cgacccccga
     8821 tgtccgggtg gtcgccccgt aggacgacac ggcgatgaag tcgatctcga ccggtaagtc
     8881 gatgcagcgg gccagatcgg tcaagaacac gtacgcgccg cgcagcacgc acaccagcag
     8941 aggggcactg ccggcgtagt ccccggtcag ttgggctccc agctcggcga tgcgggtggc
     9001 gatctgctcc tctgtcagga gcacccggcc gatcgccgga tcgtcgtcga acgaggtggg
     9061 ccccggcacg tccatcggcg tcgaccctag tgccccggca cgggggcacc gcccgtcggg
     9121 ttccccgagc cgtcgggccg acggtgtgcc cctcccgccg gtgcgaccag acgcagacgg
     9181 cccgccgaac gggccacccg ccaacctccg acgacgtcgc agccgatcgc ccgccccgcg
     9241 gccacgtcga ggatccgctg cagtgcggcc gcgtcggggc ccaggcccga tccggtgacg
     9301 tcgcgccacc accgacgcac cgcggaggac gccagcggtt ccggagcggc aaccagcacg
     9361 gccgcgtcgg ttgggtcgag gtccgcggcg agcgagtcca ccagcgccga ctgatcggcg
     9421 gccagcccgg ccaggcgaca cagcagcgga acgacgtcgc gttcggcgac ctcgtcgagc
     9481 agcggcagga cgcgatgacg gacgcggttg cgtcggtggt cccaggagtc gttggtcgga
     9541 tcgaccacga cgtcgatccg cagctgacgg cacaggtcgt gggtctcgga gcgtcgcagg
     9601 gccaggatcg gatgttggcg ggcggtcatc gccgcgagtc cggccgggcc ggttccccgg
     9661 agcagacgga gcaggacggt ctccgcctgg tcgtcggccg tgtggccgaa cagcgcgtcc
     9721 ggccccaccg cggcgtgacg ggccgtccgg gcccgggcct cgaggtcggg gcccggtgcc
     9781 acgtgtgccg tctcggcccg gaaccgggtc ccgaggcggt gggccaggtc ctcgacgacg
     9841 agcgcttcgt cggcggagcc cgggcgcagt ccgtgatcga cgtgcacggc ggtgacgtcg
     9901 aggccggcgg cgacggcgag cacgagcagc gcggaggagt ccgctccgcc cgagaccgcg
     9961 caggtgaccg acgatccgac cggcgggaac gagcaccgtc tgaggagcgc gtcgatggtc
    10021 gccccgaccg gtgcctcagg ccgtcgcaac ggctgctccg acccgacgga tccaggtgtc
    10081 ggggtcgcgg atctcggcga gcgacggcag gagctccgag cgctcgaagg cgcggttgag
    10141 cagggcgggc cccccggcgt cctcgaccgc ggcgatgaag gcctctccct gctggtactg
    10201 ggcgagcttg gcctcgagcc cgatgacgcg ctggagcagg cgggtggcca ggttcatctc
    10261 cgagcggcgg gcgcgcagca cccgggcgaa gcgtggcgcc gaggggacga ggtcggtcgc
    10321 ggcgcggtcc atgaccacgt cgccgtgccc ttcgagcagg ctcatcagcc caccgacctt
    10381 gtccatggtc agccgctgct ccggggtggc gaacagcgcc gcgatccctc cgtcgtcgag
    10441 cgggttggtg cgggtgcgga tgccctcggc cacccgaccc accgcggtga acagccgggt
    10501 cgggtcgggg tccgcggcgt cgacgagctg ctcgacgagc cccaggaagt acgggcggag
    10561 ccacgggacc ccggtgaact gggtgcgatg ggtgacctcg tgcagcgcca cccacaggcg
    10621 gaactcgccg ggcgggaagg actggcgctt ctccaggccg aggatgttgg ggcccacgta
    10681 gtagacgagg tcctggtcgt cggggtcctc ctcttcgagg accagcaggt cgtactgacc
    10741 gagcacccgg gtcgacatcc acccgaggag cgcccccaac tcggcgcccg ccacctttcc
    10801 cgagaccgcg gcggccggac cgtcgagctc ctccagacga ccgagcacgg gccgcagcat
    10861 gcgccggtag ctcgccaggt tcgcccgcac ccagtccagc cggcccacga cgcgggcccg
    10921 ggccgcaccg gcctgcgatg tgaagccggt ctcggactcg acacggggct gggcgacggc
    10981 ggtcagctcc tggagctcgt cggccatccg atccgcgtgg tacgacgcgg cgaagggctc
    11041 ctctccggcg atgcgggccg ccacgcgctc cgccagggcc cagtcgatcg acgagctcac
    11101 gattcaccgc ccgccacggt tcgacccggt ccgacccgca cgatggagtc ggagcgcgac
    11161 ccgaccgagg ttcagccctc gtcgctgggg tagcgggtgg acgtgacctt gaagaagtag
    11221 ccgaggacca ctgcgatcac gacgaagatc gcgccgagcg cgatcgggac cgcgagccag
    11281 tagtgccaca cgggcgagtc ggcaaggagc gctgcaacct gcatggcgtc cactctagga
    11341 gccatccgac ggagcggtca cacccgacac gccgtcggta ccgccgctgc tgagctcgtg
    11401 gagcagtccg cagaagcggg ccctgacctc gggtgtcacc tgctcggtgc acttggcggc
    11461 gatcacccgg cggaggtcgg cttcgaagtc gaaggcctcg aggcacggcg aacaccggcg
    11521 gaggtgcgtc tcgacctgca gcatcacccg gtcgtcgagc tccccgtcga ggaaggcgta
    11581 gagctcggcg atggcctcgt cgcagtcgga cgcgacgctg tggtcgtgac ccgggtggtc
    11641 gacctgcccg gacggatccg gacggtcggc ccggtcaccg ggggtggggg aggcggaagg
    11701 ggtcggggac atgggagcgg atgggtcggg ctgtgctcga atgctacggc tcgatcacga
    11761 ccggatcacg gtcggcgagc ccacgttcga cggcgaactg gtgcaacgaa cgctggaggg
    11821 cctttcttcc ccggtggagc cgactcatca ccgtgccgat cgggacgtcc atgatctcgg
    11881 cgatctcctt gtaggagaag ccctcgacat cggcgagcag taccgccatc cggaacgact
    11941 ccggcaggtc gtcgagcgcc tggcggacgt cgccctcggt gaacatgtcc atcagctcgt
    12001 cctcggcgct gcggccgagc tgggccgctt cgagaccgcc gaggcggcgg tagaggtaca
    12061 ggtcctcgac ctcgtcgagc tcggtctgct cggggcgacg ctgcttggcg cggtaggagt
    12121 tgatgaacgt gttggtgagg atccggtaga gccaggcccg gaggttggtg ccgtcggtga
    12181 agttggggaa cccccggaag gcgcgcacga acgtctcctg gaggaggtcc tcggcgtcgg
    12241 ccgggttccg ggtcatccgg agcgcggcgg cgtacagctg gtcgacgaag ggttcgacct
    12301 gatccgcgaa ggtggactgg tccgccatgg gagttccacc gtaggcgacc cctgtgacgg
    12361 tgtcagccga cgggtacagc gtcgcgacgg taggccccgg tccggtcgag gggcggggag
    12421 gtcagccgga gtggcgaccc accccgctgc gctgcggcga cccgccggcg tcgtagtcct
    12481 ggctgcggag ccagcgcccg cagtcgtggc accggtcggc cgtgcctgcg cacagtcccc
    12541 ggcagtggcg gcaccccgtt gtgctgctca tggccgtacc tctcgctcgg ttcggagaaa
    12601 tggcccccgt acacgacgga agcaccgtcc acggtgacgt ggacggtgct tccagtcaac
    12661 agggcctttc gacccaattc ggatcaggtg gtctcggtgc tgagggggct cactgctccc
    12721 gggaacgacg gctccaggcg aacagagcgc caccggccac gaggagaccg gcgccgatca
    12781 gggccagacg ggccgaggac gccccggtca gggcgagggc cgccgcgttc tcgccctggg
    12841 tggcggtggt gtcggcgacg gtcgccccgg tcaccacatt ggtggcggtc tgggggacgg
    12901 tggtcgccgc ctggaccgtg gcggtggtcg gcggggcggg aaccgtggtc ggggcggtgc
    12961 cgcacttctc catgggcggg aagtactgct cggaggtctt ggtgtcaccg acgaacagga
    13021 gcggaccgtg ccacgggccg gtgggagaga tggtctgcgg gctctcgccg gtgatcacgt
    13081 cgaactggac ggcgtcgcac tccttggcga acgtgatcgt ggtcgtcgag gcctcggcga
    13141 tggtgaagtc ctgccgctcg accagggtct gcggccactg gccaccggcg ggcgacgcgc
    13201 ccttgggcat ggcgtaggcc accgcggcgg ccgcgatcgg gttgcacggc ttggtggcgc
    13261 tggtgatcac gatgcggaac acgtcggcgt cggacgtggt cgtgtaggtg agctgagcct
    13321 gcgcgagcgg gttgcactga ccgtcgaacg gcaggggagc ctgagtgatc gtgatgatcc
    13381 cgtcggcctc ctcggcaggc tgctcagccg ggggtgcctc ggctccctgg ccatcgggaa
    13441 cctcgaccgg gccatcggga acctcgacgg ccggcgtcgg ggtcgctgcg gtgtcgggtg
    13501 cgtcgacgtc gtcaccggcg gctccggcgt tcgtcgtccc gacgagaacc gccgccatca
    13561 cggcgacgag gcacaccagg agcgagaacg cgcttctccg gtgggcccgg tttcgtccct
    13621 gcatgtcgtc cacttcgtac tccctcgcct cgcccagccg gtcgatccgg cccacaggat
    13681 cgcagacctg ccggattcgg acaaggagac ggggaggtcg acccacccga cccaccggtg
    13741 cagcaccggc cgggctccct ggtcgatact gaccggcgat cgacgctgac ccgtcgccca
    13801 ccgcatcgcc ggcgccggtc ggccccacgc ccaggagacc cgatgacccg agacgaactg
    13861 ctgagaacct tcgacctgac cgaccgggtc gccgtggtca ccgggggcag ccgggggatc
    13921 ggccgggcgg tggccgaggg tttcgccgcc gtcggtgccc gggtcgtggt ggcgagccgc
    13981 aaggccgacg cgtgcgacga ggcggccacc gccatccgcc gggccggcgg caccgcgctg
    14041 gcggtaccca cccacatggg cgacctcgac gccgtcgcac ggctcgtccg caccaccgtc
    14101 gaggagttcg gcggaatcga cgtggtcgtc aacaacgccg ccaaccccct cgccctcccg
    14161 atcggttcga tcacccctga cgcgctggcc aagtcccacg acgtgaacct gcggggaccg
    14221 ctcttcctgg tccaggaggc gctcgagcac ctccgcgcca gcgaccacgc ggcgatcgtc
    14281 aacgtcctga ccgccgggat cttcacccgc ggcgagtacg tctcgctgta cgtcgcggcg
    14341 aagtccgcgc tcgccgcgtt cacccgggcg atggccgccg agctggccgg cgacgggatc
    14401 cgggccaacg ggctggcgcc cggcaccgtc cgcaccgaca tggtgctgaa cacccccgag
    14461 gagttccagg cgctcgctgt cgacgcccag ctgatcaagc gcatggccga gccagaggag
    14521 atggtgcccg ccgctctctt cctggccagc gacgcgtcga gcttcatgac cggtcagacc
    14581 ctcgtcgtcg acggtggcat gaccagccac tgacgcctcg agccgatccg gtgcaccaac
    14641 acgggatcgg acgacaccaa cggcgggcta cccggtcgag ccctcctcct gtcgggccag
    14701 ctcgcggcgg tgcgcctcca cgatctcctc ctcgatctcg gcctcgacgc cctcctcctg
    14761 gcgttcggcc tggacgtcgg tgtcgcccgg ttcctgcccg ccatggggat gacgctccaa
    14821 gccgtggtgc tcagcgtcgt tcgtcatggt ggactcctac ccgttcccgg tccgcgccag
    14881 aacgaccgac cgcggcgggg ctggcgacca gtccggcgtg acgaagtcga tcgtcggcgg
    14941 gtccttccgg catgcgtccg cggcgagctg cgcatctggt tcgcgcagga accgggcccg
    15001 cagctcgcgc aggcacggcg gcggcgagaa cgccaggtcc tcgctcatgg tggggacatc
    15061 gaccaccgtg gcccgtggca gcgacgccgc catcgcgtcg gcccagtcgt ccaccccgga
    15121 cgcggacagg gcgccgaccg cgatcaggac gggcacgtcg atggtcaacg gcggcgacaa
    15181 ctcgtcgaac acgctcggca cgccccacgc ttcgcacagc cggccgaagg tcggctcgtt
    15241 cgcccccgcg aactgacgca tcgccgagtc cgagatctcg gcagtccggt tcagccgctc
    15301 gtcgtagctg caggtcgccg acaggaacgc gccgccgaac gtggagccgg cgtagaagct
    15361 cacgtcctcg tcgatgccgg cggctgcggt gagctcgtcg ctcgctcccc ggacggcgct
    15421 gggtaccagg ccgagccggg aggactgccg catggcggat tcgagtcccg ccgcgtatcg
    15481 cctgccgtcg agcagcacgc ggaacgggcc gaggcctccg agcgccgtgg tctcgacgag
    15541 caccggatcc gcgtccagct gaccgaagcg ctggaggtag gccgtggcca ggtcggggta
    15601 cgcggtccgg cacctgtcgt cggtgtcgca caggtggcgg agccgggcga aggaggccgc
    15661 gagcgatcgg gccgggtcgg ccatcaccga ctcccctggc ggagtcgggt tggtcagcag
    15721 aagcgacctg accgagcccg ggttggcccg tgcccacgcc gccgcggccg tggtggtgta
    15781 cccgcccgcc gcggcgctga cccgtcccag gtcgagagcg cggaccaggt cacgaatgtc
    15841 gtcggccgcc tcgagcatcg tgtacccctc gagctgcacc ccgccggcac gaagtcgcgc
    15901 cgcgcagcga cgggctgcgt cggcccg
//
LOCUS       DAOQOD010000065        15786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1318_length_15786_cov_15.161337, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000065 DAOQOD010000000
VERSION     DAOQOD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15786
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_15786_cov_15.161337"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1334)
                     /locus_tag="PLZ72_08105"
     CDS             complement(195..1334)
                     /locus_tag="PLZ72_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA36295.1"
                     /translation="MAYIIERKDRFYVVAYDGLDPLTGKERRRWHPAGGDRHEAEAMA
                     ARIERDAAGTAPRRGGPVHLGEFLTDTWLPTKRRHVRASTSYRYSWMVDNYINPSIGH
                     VPLRRLRADHLDGLYDQLATTGGRHRTGLAPKTVHEVHVIVRSGLDLARRRQLVDSNV
                     AHAANARHKRQTRTVPRSWTAAELASFLASAGHHRLYPALHLTACTGMRRGEVAGLRW
                     SDLDRANQRLSISRTLQSIAGQPVEVPVKTRTSRRCIDLDDATIDVLARWRRRLAREG
                     LPHGADDWMFVNTGGRFVNPESLSQLFDRLQRAMPDLARIRFHDLRHTHASLLIMDGV
                     PVKVVSERLGHANVAFTIHTYQHLLPGMSAAAAQQFAALLAAHAR"
     gene            complement(1447..2310)
                     /locus_tag="PLZ72_08110"
     CDS             complement(1447..2310)
                     /locus_tag="PLZ72_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36296.1"
                     /translation="MSHRPSPPPPQVIDLPGQSGITIRALDWGGDGPPVVLLHPNGFC
                     GGLYEPIAHCIAAAARPIALDLRGHGGSTAPDHPGAYRFENLAIDVLEVLDHLDLPTV
                     AGVGGSLGGAVAILVHRLDPQRWTRLLLAEPVAFPTDAFPHQHENPMAAAARRRRPTF
                     ATRAEMTGTYRSKEPLSQLAPEALDAYIRWGTTVDASGVHLACRPEIEATIFEVSAAA
                     GGAAHAWDHLPDLTCPTTIVAGEHTFLPDIFAAQAKRARADLITVPGGHFVLHENTTR
                     GADLIAREALP"
     gene            complement(2336..3367)
                     /locus_tag="PLZ72_08115"
     CDS             complement(2336..3367)
                     /locus_tag="PLZ72_08115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA36297.1"
                     /translation="MKLDLMTGGTRLNTIQQLARHAAESGHSGLVITEAGRTAYLSCA
                     AAALAADIDLLTGIAVAFPRSPMVTAQIAWELADATDGRFRLGLGTQVRAHIERRYSA
                     TFDPPGPRIRDYIGAVRACFDAFRGASPLDYHSDHYELTLLPPIWSPGPISAPDPPID
                     LAAVNPWMLRLAGRVADGVHVHPLNTRTYLTDTVAPQLAAGAQLAGRHLDHFEIIVPA
                     FLVVGDTTDERDRWRELARTQIAFYGSTPNYAFIFDDIGHPGTTAALRDHQRAGDIAG
                     MSAVITDDILRHFVTEGTWSTIADAIADRYAGLATRVVNYFGAIAWTEDPQHLSRWKP
                     IAESLAAIP"
     gene            complement(3480..4586)
                     /locus_tag="PLZ72_08120"
     CDS             complement(3480..4586)
                     /locus_tag="PLZ72_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA36298.1"
                     /translation="MTTTSNQDLIGREGVNDLDAILAMTNTDIDSAVHAITDNAEAIF
                     TWDYEKGARPGLNKLYEKAKTAQWNGETDLPWDTDVDLEQVAKLLLPSFGPDQMDVAN
                     TPLATWGDAEWLQLGMESQVWALSQFMHGEQGALLCTAKIVETVPWIDAKYYAATQVM
                     DEARHVEVFAKYLDTKLEGYYPINTHLGMLLDDIVADARWDMTYLGMQIMVEGLALAA
                     FGFMHMLTEEPLLKQLLRYVMADEARHVAFGVLSLKEYYAELTDNELAERQEFAFEAA
                     VRMRDRFLQQEVWDRLGIDPKVAIPLTQASPIRSEFQKMLFTKIVPNCKKLGLLDANN
                     AWLRRRFEELGVIQFEDWVDTEAEYEAFALAEIK"
     gene            complement(4631..5482)
                     /locus_tag="PLZ72_08125"
     CDS             complement(4631..5482)
                     /locus_tag="PLZ72_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36299.1"
                     /translation="MTLEGTDGVRLAADRRGDPDQPVVVLLHGAGQTRHAWLRTTERL
                     SNEGFCTVAVDLRGHGDSDWAPDGDYRRRAFAADIELIRSQLGPIAAVVGASLGGITA
                     LLAHDQRPGGITNALVLVDIAPRIEQTGADRILAFMADRPEGFQSVEEVAEAVAAYNP
                     HRPAPKDLDGLKRNLRLGDDGRYRWHWDPKLIDGPTSLISARDPEALHAAARRLRVPC
                     LLVRGRHSDLLTEDGAAEFRRAVPHAEFVDINGAGHMVAGDTNDPFTEAVAAFLAPLA
                     QRQRESA"
     gene            complement(5500..6936)
                     /locus_tag="PLZ72_08130"
     CDS             complement(5500..6936)
                     /locus_tag="PLZ72_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNA36300.1"
                     /translation="MNNDNVVAELDSIPASVPHCDRFVELLHGEGMSSPVSEITSARV
                     GRTATAARAATTVLIAGRHAARAAVGPIVHRGLLSRPDWSPTAAREARRAFTELGPTY
                     VKLAQLIASSPGLFPEVLSDELRTLLDRVPPLAPVDVDHVIRRELGGAPGDVFGRFDP
                     VPLASASIAQVHAATLHDGTDVVVKVQRRGIRARLAADLSILAGAAHLLERRSARARM
                     ANPVAVVEDFSATLAHELNFVLEAQAMERFADNLKTFGENSGVRVPAVHWRFTTPRVL
                     TMEQIHGHSIDDTEALRATGFDLAELLKRGVRAWMEAALHHGFFHGDVHAGNLMVDTA
                     GNVVFLDFGIVGQLDDRTKAIIRNGLPALLIDGDFVEVAKSIYDLGAVLKPVDLDQAS
                     ADIAEIVEPILAKPLSEISYGQVLIDIVRIGTRYEVRLPRELVLVAKQLLYFERYAKL
                     MAPDWNILDDPELIGFLFESVHAERPAS"
     gene            6899..7522
                     /locus_tag="PLZ72_08135"
     CDS             6899..7522
                     /locus_tag="PLZ72_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36301.1"
                     /translation="MESSSATTLSLFTLPVDHQGIPSAPSAELDPYLDAAARCFVRYG
                     VRRTSVQDVATELGVNRTTVYRQVGTIDNQIRLLIARDLHRLLAELPASLADKSGPRA
                     VVELMAAIIGYASKHPVLAKVLADEPEVIGPFLASDLPEVIARIKSALAPLLQAAMAT
                     SQIATRDADLLAESLVRLGITLILAPPPGDLGTFLAEVLVPTLKPPP"
     gene            complement(8100..9302)
                     /locus_tag="PLZ72_08140"
     CDS             complement(8100..9302)
                     /locus_tag="PLZ72_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36302.1"
                     /translation="MNRPPVTDWTADFDHLDPTFVADPYPIYEQLRTDCPIAHTDRYH
                     GVWIPTRYEDLAAIAHDHHHFSSRTILVTDRPESPHDLGFHAPPITEDPPYHTDIRRL
                     LLPVFSPKVVADYEPITRAMANDLIDGFITDGGCDAATDFAQHVPIKVITRMLGIPDS
                     DHAQFRDWIHRLIEESPIAGDATFEAIFEIGGYLAGHVDQHRTSPRDDIITHLLEARM
                     PDGRPLTDEEILGVCILLLLAGIDTTWSAIGSSLLHLATNPDDRRRLRDEPDLINSAT
                     EEFLRVFAPVTMAREVIEDTEVGGCPMRKGDRLLLPFPSGNRDPEIFDRPDNVVLDRE
                     ANRHFAFGVGIHRCLGSNLARMELRVALQTWLERIPEFTLTDPDAVTWSAGQVRGPRT
                     LPFAFTAG"
     gene            complement(9316..9507)
                     /locus_tag="PLZ72_08145"
     CDS             complement(9316..9507)
                     /locus_tag="PLZ72_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNA36303.1"
                     /translation="MRVVVDAERCQGHNRCHALAPVLFDIDDLGYATAAPGSVPADLE
                     AAARLAEANCPEHAITIDP"
     gene            complement(9504..10592)
                     /locus_tag="PLZ72_08150"
     CDS             complement(9504..10592)
                     /locus_tag="PLZ72_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36304.1"
                     /translation="MTGTPRLRYTRDEILTDPDYTRRIERAGVLLHGGYGPDGTYLPP
                     RSTHRIPAIAAWTAKLAAAGHPTRVIDPDAVDHAFVPNTEQAKLLLRNGAAGAMTRIL
                     TLIGVVEGFGNDGIKLIPPMDLQSHIVEPIAGTCLGHLFDGLLEAHGNDEAGRGPEAG
                     HDQMWFAVRDAALADPPITIDMFENLPIAPPPGYEGPARPSPEAITVGGMLDGLRDDV
                     APELQLLVRAMIQILVIELLAYHTFAWASEVLGDPECSHDAGFARDTIDHIRIDEDIH
                     VAYLQCALAELATLTVRTVSGTTVPGADLVNAARRAALDNQTGDRFDRILAYRLAQVR
                     SELAAHPDGARLTTEFDALAAKPAEALT"
     gene            complement(10589..11203)
                     /locus_tag="PLZ72_08155"
     CDS             complement(10589..11203)
                     /locus_tag="PLZ72_08155"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36305.1"
                     /translation="MREVTDNIAAALPAASAVFAARGLDGTRMDDIVEATGIPRPTLY
                     YHFSSKEEILAWLLERLLRDLSVEIGHILDRDELARDRLHAVFGAYLGLFAKHRELCT
                     VLLTELGRITRIPALADAIWASFHEPVRKLLHAGEIDGSLRPIDDDVTASAIFGAVTM
                     VGLHYVVTGQPIDSDAAAGRLDDLLLGGLAPPTRPRTSTKGRKR"
     gene            complement(11200..12465)
                     /locus_tag="PLZ72_08160"
     CDS             complement(11200..12465)
                     /locus_tag="PLZ72_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36306.1"
                     /translation="MTDTLSAEPPPMSPDGLVMETLLTPEGRADPYPRYRLLRDAAPV
                     HHSELGPVWFLTRWSDCNNVLRDPRLGKGSLDADRAALFSPGLPPRQRSVMGNSMLFV
                     NPPDHTRLRGLVSRGFTPRQIARLEAHIGHMADVIVERMAEAGEVDVMDALAFRLPVQ
                     VIGELVGVPPSDRDQFRVLVRNAAAALEPGVTEIQLDAAEHAMAVMDDYFRGLIARRR
                     RDLDHDLTSALIAARDGDDRLTEDEMVATLILLFSAGFETTTNLIGNGLLCLLRNPDQ
                     FDRLRADPALVDSAVEEMLRFESPVQVDARTAFEPVEIDGHTITTGETVVTFLGAANR
                     DPAVFPDPDRFDVTRHPNHPLSFAAGIHYCLGANLARLEGRVVFDRLTRRFADIAWLD
                     NGPDWRGTLILRGVNHLNVAVTPKEGTTR"
     gene            complement(12475..13464)
                     /locus_tag="PLZ72_08165"
     CDS             complement(12475..13464)
                     /locus_tag="PLZ72_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA36307.1"
                     /translation="MREPLVPTLDRLPESVRRFELPTPFPVGPVNCYLLLDDPVTVVD
                     PGMLYADSVATLTAELAKVGLDAADVGAVVVTHAHPDHYGTAGWLAERAGAPIYAGRA
                     EVPKLLENRDRDQLYTLIRSFGIPEEMLGVFPAFYGAVREWLHDIDESRVIAVDDGDE
                     LRLGGRTLQALVTPGHAAGHLSLWEPVSSRLLSGDHLLPAITPNPLVELDGDSDLLRR
                     RSLVDYLGSLDRFEALDPQVVLPGHGPAFTSVGALVTATRAHHERRAEEIHAHISRLV
                     EPTAYELSRAVFPHIDGFEILLAISEVLGHVDLLVADGDVVIDLGSPTRYAST"
     gene            13578..>15786
                     /locus_tag="PLZ72_08170"
     CDS             13578..>15786
                     /locus_tag="PLZ72_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA36308.1"
                     /translation="MTSALHRLGRWCAHRPWRVIALWMLAAAAVLTAGARWGAASSES
                     IEIPGTESQRAIDLLEERYPDAAGGRARVVFATDAGRVTDPQFRSGAQGTLDQIRALP
                     RVRQVIDPFGPAGGFLVSPDGRIAFAEIVYAVTAREVGVDGVDALTATAGPARDAGLD
                     VAFGGPVVERIGPDDTHASELIGLAVAVIVLAVAFGSVTAMALPMCTSLVGLGVGLAL
                     MRLLGGFVEVTSVAPILGSMIGIAVGIDYSLFIVTRHRQQLAQGYELDESVARALATS
                     GQAVVFAGVTVMIAILGLALIGIPLVAALGYAVSLMVAIAVLVAITLLPALLGLVGTR
                     IDRLRVPGVQLRYESDHHTSRSTSARWARLVTRRPWPFLAAGSVLLVALAVPAARLEL
                     GWPDAGNLPPDTTARHAYDLLAEGFGEGFNGPLLIAVDLREVADPPGALATISTAVAA
                     TPGVQFASPAQTNDTGDTAVIQVVPTTSPQDPATEDLVDRLRGETVPTLEADTRTVIE
                     VTGTTAAFIDISKRLADRLAVFIGAVVLLSFLLLTAVFRSPLVALKAALVNLLGIAAA
                     YGALVAVFQWGWGLDAVGLDETVPIVSFLPMMMFAILFGLSMDYEVFILSRVREEWVA
                     TGDPTGSVVGGIATSARVITAAALIMISVFASFVLGDTPEIKMFGFGLAVAVALDATV
                     IRMVVVPATMTLLGSRNWWLPGWLDRILPNLDIEGTGTLGPNPAVAGRDAAHPD"
BASE COUNT         2240 a         4861 c         6046 g         2639 t
ORIGIN      
        1 gctcgtcggg ctcataaccc gaaggtcgca ggttcaaatc ctgcccccgc caccaactga
       61 atgtgcaggt caggccccac ttttcggagt ggggcctgct gcttgtctgg ggtgacgtct
      121 accgggtgat ctaccggctc ggcgggacct ggcccgctga cctgggcgtt cgctggagtc
      181 gtgccggtag acgtctaccg ggcgtgggcg gcgaggagcg ccgcgaactg ctgcgcggcg
      241 gcggcgctca tgccggggag caggtgctgg taggtgtgga tggtgaaggc gacgttggcg
      301 tggccgagcc gttcgctgac gaccttgacc ggtacgccgt ccatgatgag cagcgaggcg
      361 tgggtgtggc ggaggtcgtg gaaccggatg cgggccaggt cgggcatggc tcgctggagg
      421 cggtcgaaga gctggctgag cgattcgggg ttgacgaacc ggccgccggt gttgacgaac
      481 atccagtcat ctgcgccgtg gggcagtcct tctcgggcga ggcgtcgccg ccagcgggcg
      541 agcacgtcga tggtggcgtc gtcgaggtcg atgcagcggc ggctggtgcg ggtcttgacg
      601 gggacctcga caggctgacc ggcgatgctc tggagggtgc ggctgatcga gaggcgctgg
      661 ttggcccggt cgaggtccga ccagcggagc ccggcgacct cgccgcggcg catgccggtg
      721 caggcggtga ggtgcagcgc cgggtagagc cggtggtggc cagccgaggc caggaacgac
      781 gccagttcgg cggcggtcca ggaccgggga accgtgcggg tctggcgctt gtggcgggcg
      841 ttggcagcat gggcgacgtt gctgtcgacg agctggcggc gccgggcgag gtcgaggccg
      901 gaccggacga tcacgtggac ttcgtgcacg gtcttgggag cgaggccggt gcggtgacgt
      961 ccgccggtgg tggccagctg gtcgtagagg ccgtcgaggt ggtcggcgcg caggcgccgg
     1021 agcgggacgt gcccgatgga agggttgatg tagttgtcca ccatccacga gtagcggtac
     1081 gaggtggatg cccggacgtg gcgacgcttg gtcggtagcc acgtgtcggt gaggaactcg
     1141 ccgaggtgca cgggtccgcc gcggcgtgga gcggtgccgg cggcatcgcg ttcgatgcgc
     1201 gccgccatgg cttcggcctc gtggcggtca ccgccggcgg ggtgccatcg gcgtcgctcc
     1261 ttgcccgtga gcgggtcgag gccgtcgtag gcgacgacgt agaagcggtc cttgcgttcg
     1321 atgatgtagg ccatcgcccg agcttggcca cttcgggacc aacccgacga gcctgctggt
     1381 gccgtgacca gggacttcca ccgctgggcg tcgagtgatc accggtcgac tcgatccggt
     1441 ctctcgtcat ggaagtgctt cgcgagcgat gaggtcggcg ccgcgggtgg tgttctcgtg
     1501 caggacgaag tggccgccgg ggacggtgat gaggtcggca cgggctcgct tcgcctgggc
     1561 ggcgaagatg tcgggcagga aggtgtgctc gccggcgacg atcgtggtgg gacaggtcag
     1621 gtcggggagg tggtcccagg cgtgggcggc gccgcctgcg gcggctgaga cctcgaagat
     1681 cgtcgcttcg atctcgggcc ggcacgcgag gtgcacaccg ctggcgtcga cggtggttcc
     1741 ccaccggatg taggcgtcga gggcttcggg agcgagctgg gacagcggtt ccttgctgcg
     1801 gtaggtgccg gtcatctcgg cgcgtgttgc gaaggtgggt cgccggcgcc gcgccgctgc
     1861 ggccatgggg ttctcgtgtt ggtgggggaa ggcgtcggtg gggaatgcca cgggctcggc
     1921 gagcagtagc cgggtccagc gttgggggtc gaggcggtgc acgaggatgg caacggcacc
     1981 gccgagagat ccgccgacgc cggcgaccgt ggggaggtcg aggtggtcga ggacctcgag
     2041 gacgtcgatg gcgaggttct cgaatcggta ggcgccgggg tggtcgggcg cggtgctgcc
     2101 gccgtggccg cgcaggtcga gggcgatggg gcgggcggcc gcggcgatgc agtgggcgat
     2161 gggctcgtac agcccgccgc agaagccgtt ggggtgcaac agcacgaccg gtggcccgtc
     2221 gccgccccag tcgagtgccc ggatggtgat gccggactgg ccggggaggt cgatgacctg
     2281 gggtggtggt ggcgatggtc ggtgcgacat ggggggcgcc gttggggtcg cgccgtcagg
     2341 ggatggccgc gagcgattcg gcgatgggct tccagcggga gaggtgctgg gggtcctccg
     2401 tccaggcgat ggcgccgaag tagttgacga cgcgggtggc gaggccggcg tagcggtcgg
     2461 cgatggcgtc agcgatggtg gaccaggttc cttcggtgac gaagtggcgg aggatgtcgt
     2521 cggtgatgac ggcgctcatc ccggcgatgt cgccggcgcg ctggtggtcg cgcagcgcgg
     2581 cggtggtgcc ggggtggccg atgtcatcga agatgaacgc gtagttgggg gtcgagccgt
     2641 agaaggcgat ctgggtgcgg gcgagctcgc gccatctgtc ccgttcgtcg gtggtgtcgc
     2701 cgacgacgag gaaggcgggg acgatgatct cgaagtggtc gaggtggcgg ccggcgagct
     2761 gcgcgccggc ggcgagctgc ggtgcgacgg tgtcggtgag gtaggtgcgg gtgttgaggg
     2821 ggtggacgtg gaccccgtcg gcgacccggc ccgcgaggcg cagcatccac gggttgaccg
     2881 cggcgaggtc gatgggcggg tccggcgcgc tgatgggccc gggggaccag atgggtggca
     2941 ggagggtgag ctcgtagtgg tcgctgtggt agtcgagcgg ggaggcgccg cgaaacgcgt
     3001 cgaagcacgc ccggacggcg ccgatgtagt cgcggatgcg ggggccgggc gggtcgaagg
     3061 ttgcgctgta gcggcgttcg atgtgggcgc ggacctgggt gccgagcccg aggcggaacc
     3121 ggccgtcggt ggcgtcggcg agctcccagg cgatctgggc ggtgaccatg gggctgcggg
     3181 ggaacgcgac ggctatcccg gtgagcaggt cgatgtcggc ggcgagggcg gcggccgcgc
     3241 acgacaggta ggcggtgcgg ccggcttcgg tgatgacgag cccggagtgg ccgctctcgg
     3301 cggcgtgccg ggcgagctgc tggatggtgt ttaggcgcgt gccgccggtc atgaggtcga
     3361 gcttcatggc gcgctcctca cgtagtgaat gcgatccgct gggtgggttc cccctgccac
     3421 catcgaggaa tgctgttggg ggccacttcg acaggtcggg tcatccacgt ccccgggtgc
     3481 tacttgatct cggcgagcgc gaatgcttcg tattctgctt cggtgtcgac ccagtcctcg
     3541 aactggatga cccccagctc ttcgaagcgg cggcggagcc atgcattgtt ggcgtcgagc
     3601 aggccgagct tcttgcagtt ggggacgatc ttggtgaaga gcatcttctg gaactcgctg
     3661 cggatgggcg aggcttgggt gagcgggatc gcgaccttcg ggtcgatgcc gaggcggtcc
     3721 cagacctctt gttggaggaa ccggtcgcgc atgcggacgg cggcctcgaa cgcgaactct
     3781 tggcgttcgg caagctcgtt gtcggtgagc tcggcgtagt actccttgag gctgagcacg
     3841 ccgaaggcga cgtggcgggc ttcatcggcc atgacgtagc ggaggagctg tttgaggagg
     3901 ggttcctcgg tgagcatgtg catgaacccg aacgcggcga gggcgaggcc ttcgaccatg
     3961 atctgcatgc cgaggtaggt catgtcccac cgggcgtcgg cgacgatgtc gtcgagcagc
     4021 atgccgagat gagtgttgat ggggtagtag ccctcgagct tggtgtcgag gtacttcgcg
     4081 aacacttcga cgtggcgggc ttcgtccatg acctgggtgg cggcgtagta cttggcgtcg
     4141 atccagggga cggtctccac gatcttcgcg gtgcagagca gagcgccctg ttcgccgtgc
     4201 atgaactggc tgagcgccca tacctgggat tccatgccga gctgcaacca ttcggcgtct
     4261 ccccaggtgg ccagcggggt gttggcgacg tccatctggt cgggcccgaa cgatggcagc
     4321 agcagcttgg ccacctgctc gaggtcgacg tcggtgtccc acggaaggtc ggtctcaccg
     4381 ttccactgcg cggtcttggc cttctcgtag agcttgttga gcccgggccg agcgcccttc
     4441 tcgtaatccc aggtgaagat ggcttccgcg ttgtcggtga tggcgtgcac cgccgagtcg
     4501 atgtcggtgt tggtcatggc caggatggcg tcgaggtcgt tgacgccctc gcggccaatg
     4561 aggtcctggt tggaggtggt ggtcatgttg gggctccttg ggaggcgtga acgacgaggg
     4621 tggactctgg tcatgcgctc tcgcgctggc gttgtgcgag aggggcgagg aacgcggcaa
     4681 cagcttcggt gaaggggtcg ttcgtgtcgc cggcgaccat gtggccggca ccgttgatgt
     4741 ccacgaactc cgcgtggggg acggcccggc ggaactccgc tgcgccgtct tcggtgagca
     4801 ggtcgctgtg gcggccccgc acgagcaggc atggcacgcg cagtcgtcgc gctgcggcgt
     4861 gcagggcttc ggggtctcgg gcgctgatca ggctcgtggg gccgtcgatg agctttgggt
     4921 cccagtgcca gcggtagcga ccgtcgtcgc cgaggcgaag gttgcgcttc aaaccgtcga
     4981 ggtctttggg ggcggggcgg tgcgggttgt aggcggcgac cgcttcggcg acttcttcga
     5041 ccgattggaa gccttcgggt cggtcagcca tgaaggcgag tatccggtcg gcgccggttt
     5101 gctcgatgcg gggcgcgatg tcgacgagga cgagcgcgtt cgtgatcccg ccagggcgct
     5161 ggtcgtgcgc gagcagcgcg gtgatcccac ctagggacgc gccaacgacc gcggcgatcg
     5221 ggccgagctg ggagcggatc aattcgatgt cggcggcgaa cgcccgtcgg cggtagtcac
     5281 cgtcgggcgc ccagtcgctg tccccgtggc cccgcaagtc gacggcgacg gtgcagaacc
     5341 cttcgttgct gagccgttcg gtggttcgta gccacgcgtg gcgggtctgc ccggcaccgt
     5401 ggagcaggac gacgacgggc tggtccgggt cgcctcgccg gtcagccgcg agccggacac
     5461 cgtcggtccc ttcgagggtc atcctcgtcg ccgtgttgct catgaggcgg gccgttcggc
     5521 gtggacgctc tcgaagagga agccgatgag ttcggggtcg tcaaggatgt tccagtcggg
     5581 ggccatgagc ttggcgtaac gctcgaagta gagaagctgc ttcgcgacga ggacgagctc
     5641 acggggcagg cgcacctcgt agcgtgtacc gatccggacg atgtcgatga gcacctggcc
     5701 gtaggagatc tccgacagcg gcttggcgag gatcggttcc acgatctcgg cgatgtcggc
     5761 cgacgcctgg tcgaggtcaa ccggcttgag gacggcgccg aggtcataga tcgacttggc
     5821 gacttcgacg aagtcgccgt cgatgagcag agcggggagc ccattgcgga tgatggcctt
     5881 ggtgcggtcg tcgagctggc cgacgatacc gaagtccaag aacacgacgt tgccggcggt
     5941 gtcgaccatg aggttcccgg cgtggacgtc gccgtggaag aacccgtggt gcagcgcagc
     6001 ttccatccaa gcgcgcacgc cccgcttcaa cagttcggca agatcgaacc cggtggcccg
     6061 gagcgcctcg gtgtcgtcga tgctgtggcc atggatctgc tccatggtga gcacccgcgg
     6121 cgtcgtgaac cgccaatgca ccgcggggac acggacgcca gagttttcgc cgaaggtctt
     6181 gaggttgtcg gcaaagcgtt ccattgcctg ggcttcgagc acgaagttga gctcatgggc
     6241 gagtgtcgct gagaagtcct cgacgaccgc gacggggttt gccatccttg cccgggccga
     6301 ccgccgctcc aacaggtggg cagcgccggc aaggatcgac aggtcggcgg cgagtcgggc
     6361 tcggatgccg cgacgttgca ccttgaccac gacgtcggtg ccgtcgtgca gcgtcgccgc
     6421 atgcacctgt gcgatcgacg ccgaggccaa cggaaccgga tcgaaccgcc cgaagacgtc
     6481 accgggggcg ccgccgagct cgcggcggat gacgtgatcc acatcgaccg gcgcgagcgg
     6541 cggcacacgg tcgagcaacg tccgcaactc gtcggacagc acctcgggga acagcccggg
     6601 gctcgacgcg atgagctgcg ccaacttcac ataggtgggc cccaactcgg tgaaggcacg
     6661 acgggcttca cgcgccgcgg tcggactcca gtccggccgc gagagcaacc ctcggtgcac
     6721 aatcgggcca accgccgctc gagccgcatg gcgaccggcg atcaacacgg tggtcgcggc
     6781 gcgggcggcg gttgcggtgc gcccgacacg tgcagaagtg atctcgctga cgggactgga
     6841 catgccctct ccgtgcaaca actcgacgaa tctgtcgcag tgtggcacac tggccgggat
     6901 ggagtccagc tccgcgacga cgttgtcgtt gttcaccctg cccgtggacc accaagggat
     6961 cccgagtgct ccgagcgcgg agctcgaccc gtacctcgat gccgcagcca ggtgtttcgt
     7021 gcgctatggc gtgcggcgaa cctcggtgca ggacgtcgcg accgagctgg gcgtgaatcg
     7081 cacgacggtc taccgccagg tcggcacgat cgacaatcag atccgcctcc tgatcgcccg
     7141 cgacctgcat cggctgctcg ccgagctgcc agcgtcgctt gctgacaaga gcggacctcg
     7201 ggccgtggtc gagttgatgg cggcgatcat cggttatgcc tccaagcacc cggtgctggc
     7261 caaggtcctc gccgacgaac ccgaggtgat cggaccattc cttgcctccg accttcccga
     7321 ggtcatcgcc cgcatcaagt ccgccctcgc accgctgctg caggcggcga tggcgacgag
     7381 ccagatcgcc acccgggacg ccgatctgct cgccgagtcc ctggtccggc tgggcatcac
     7441 cttgatcctc gccccaccgc cgggcgacct cggcacgttc ctcgccgagg tgctcgtccc
     7501 caccctcaaa ccacctccct gacacctggt caccgccgag tgctcagctc agctggtcgg
     7561 gaatcaacgc gtggctgcgt gttgagcggg tggatctgga tgccgtccgc gacctccgag
     7621 gcgtctctca ggccgatcac gtcacattgc tcctggtatc gcgccggccc tgccggcggc
     7681 gttgcacgaa acgcccgaga gcggtcgccg atgcgacagc tgccagagct cccgagagca
     7741 gcccgacaac cactcctagg ccgatgcttg gcttggactc cacgatgacg cgcccggcga
     7801 cacggtcgct cacttcgtcc acgagcgctt gaacaccggg aaccctgaat agcaccgttg
     7861 gcacgccgat ctcctgagct gcggcccgct gcagctcgtg gtcgcttggg cgcgatgtcg
     7921 cgatcgtgag cgtcgccatc aacacagcga caactgcgga ggcgaacgcg gcgcctgcga
     7981 tcgccgcgat gccaattggt cgagacgcac ttgatccact atcgggtcga tcaaggctcg
     8041 tcactgcgct actccgttcc gtccccgtgt gagcacgggt gatcagccgt cgtcgctccc
     8101 tacccggcgg tgaacgcgaa gggcagggtg cgggggccgc ggacctggcc ggcggaccag
     8161 gtgacggcgt cggggtcggt gagggtgaac tcggggatgc gttcgagcca ggtttgcagg
     8221 gcgactcgta gttccattcg ggcgaggttg gagccgaggc agcggtggat gccgacaccg
     8281 aaggcgaagt gccggttggc ttcgcggtcg aggacgacgt tgtcggggcg gtcgaagatc
     8341 tcggggtcgc ggttgcccga ggggaacggc aggagcaggc ggtcgccttt gcgcatgggg
     8401 caaccgccga cttcggtgtc ttcgatgact tcgcgggcca tggtcacggg cgcgaacacg
     8461 cggaggaact cctcggtggc ggagttgatg agatcgggtt cgtcgcggag gcggcggcgg
     8521 tcgtcggggt tggtggcgag gtgcagcagt gaggagccga tggccgacca ggtggtgtcg
     8581 atgccggcga ggagcagcag gatgcacacg ccgaggatct cctcgtcggt gagtggccgg
     8641 ccgtcgggca tgcgggcctc gaggaggtgg gtgatgatgt cgtcgcgggg gctggtccgg
     8701 tgctggtcga cgtgtccggc aaggtagccg ccgatctcga agatggcttc gaaggtggcg
     8761 tcaccggcga tggggctttc ttcgatgagg cggtggatcc agtcgcggaa ctgggcgtgg
     8821 tcgctgtcgg ggatgccgag catgcgggtg atgaccttga tggggacgtg ctgcgcgaag
     8881 tccgtggcgg cgtcgcatcc gccgtcggtg atgaacccgt cgatgaggtc gttggccatg
     8941 gcccgggtga tgggctcgta gtcggcgacg accttcgggg agaagactgg gagcaggagc
     9001 cggcggatgt cggtgtggta cggggggtcc tcggtgatgg gcggggcgtg gaatccgagg
     9061 tcgtggggtg attcggggcg gtcggtgacc aggatggtgc gggaggagaa atggtggtgg
     9121 tcgtgggcga tggcggcgag gtcctcgtag cgggtgggga tccacacgcc gtggtaccgg
     9181 tcggtgtgcg cgatcgggca gtcggtccgg agctgctcgt agatcgggta ggggtcggcg
     9241 acgaaggtgg gatcgaggtg gtcgaagtcg gcggtccagt cggtgacggg tggtcggttc
     9301 atcgctggtc tccggtcagg ggtcgatggt gatggcgtgt tcggggcagt tggcttcggc
     9361 gagccgggcg gcggcttcga ggtcggcggg gaccgacccg ggtgcggcgg tggcgtagcc
     9421 gaggtcgtcg atgtcgaaca ggacgggggc gagcgcgtgg cagcggttgt ggccctggca
     9481 gcgctcggcg tcgacgacga ctctcatgtg agggcttcgg cgggcttggc ggcgagggcg
     9541 tcgaactcgg tggtgaggcg ggcgccgtcg gggtgcgcgg cgagctcgga gcggacctgg
     9601 gcgaggcggt aggcgaggat ccggtcgaac cggtcgccgg tctggttgtc caatgccgcc
     9661 cggcgggcgg cattgacgag atcggcgccg gggacggtgg ttccactgac ggtgcgcacg
     9721 gtcagcgtgg cgagctcggc cagcgcgcat tggaggtagg cgacgtggat gtcttcgtcg
     9781 atgcggatgt ggtcgatggt gtcgcgggcg aagccggcgt cgtggctgca ctcggggtcg
     9841 ccgagcacct cggacgccca cgcaaaggtg tggtaggcga gcagctcgat gacgaggatc
     9901 tggatcatcg cccgcacgag cagttgcagc tcgggggcga cgtcgtcgcg caggccgtcg
     9961 agcatgccgc cgacggtgat cgcttcgggt gatggtcttg cggggccttc gtagccgggc
    10021 ggcggcgcga tgggcaggtt ctcgaacatg tcgatggtga tcggggggtc ggcgagcgcg
    10081 gcgtcgcgca ccgcgaacca catctggtca tggccggctt cggggccgcg accggcttcg
    10141 tcgttgccgt gggcctcgag gaggccatcg aagaggtgcc cgaggcaggt gccggcgatc
    10201 ggttcgacga tgtgggactg gaggtccatc ggggggatga gcttgatgcc gtcgttgccg
    10261 aacccttcga cgaccccgat gagggtgagg atgcgggtca tggcgccggc tgcgccgttg
    10321 cgcagcagca gcttggcttg ttcggtgttg ggtacgaagg cgtggtcgac ggcgtcgggg
    10381 tcgatgacgc gggtggggtg cccggcggcg gcgagcttcg cggtccatgc cgcgatggcg
    10441 gggatgcggt gggtggaccg gggcgggagg taggtgccgt cgggtccgta gccgccgtgg
    10501 agcagcacgc cggctcgttc gatgcgccgg gtgtagtcgg ggtcggtgag gatctcgtct
    10561 cgggtgtagc gaagccgtgg cgtgccggtc atcgtttccg tcctttggtg gaggttcggg
    10621 gtcgggtcgg gggagcgagc cctccgagga gcaggtcgtc gaggcggccc gcagcggcat
    10681 cggagtcgat gggttggccg gtgacgacgt agtggaggcc gaccatggtc actgcaccga
    10741 agatcgccga tgcggtcacg tcgtcgtcga tcgggcgcag tgacccgtcg atctcgccgg
    10801 cgtggagcag cttgcggacc ggttcgtgga agctcgccca gatggcgtcg gccagcgcgg
    10861 ggatgcgggt gatgcggccg agctcggtga gcagcacggt gcacagctcg cggtgctttg
    10921 cgaacaggcc gaggtaggcg ccgaagacgg cgtggagccg gtcgcgggcg agttcgtcac
    10981 ggtcgaggat gtggccgatc tccaccgaga ggtcacgcag gagccgttcc agcagccagg
    11041 cgaggatctc ctctttggag gagaagtggt agtagagcgt cgggcggggg atgccggtgg
    11101 cttcgacgat gtcgtccatg cgggtgccgt cgagaccccg ggccgcgaac accgccgagg
    11161 cggccgggag cgcggcggcg atgttgtcgg tgacctctct catcgtgtgg tgccttcctt
    11221 gggggtgacg gcgacgttga ggtggttcac gccgcgcagg atcagcgtcc cacgccaatc
    11281 ggggccgttg tcgagccacg cgatgtccgc gaagcggcgg gtgaggcggt cgaagacgac
    11341 ccggccttcg agccgggcga ggttggcgcc gaggcagtag tggatcccgg cggcgaagct
    11401 gagcgggtgg ttcgggtgcc gggtcacgtc gaagcggtcg gggtccggga acaccgcagg
    11461 gtcgcggttg gcggcgccga ggaacgtcac gacggtctct ccggtcgtga tggtgtgccc
    11521 gtcgatctcg acgggttcga acgcggtgcg ggcgtcgacc tgcacggggc tctcgaagcg
    11581 cagcatctcc tcgaccgcgg aatcgacgag ggcggggtcg gctcgcagcc ggtcgaactg
    11641 gtcagggttt cgcagcaggc acagcagccc gttgccgatg aggttggtgg tggtctcgaa
    11701 ccctgcggag aacaacagga tgagggtggc aaccatctcg tcctccgtca ggcggtcgtc
    11761 gccgtcgcgt gcggcgatca acgccgaggt caggtcatgg tcgagatcgc ggcgacgccg
    11821 ggcgatgagc ccacggaagt agtcgtccat gaccgccatg gcatgctcgg cggcgtcgag
    11881 ctggatctcg gtgacgcctg gttcgagggc ggcggccgca ttgcgcacga ggactcggaa
    11941 ctggtcacgg tccgacggtg ggacgccgac gagctcgccg atgacctgca ccggcagccg
    12001 gaacgccagg gcgtccatca cgtcgacctc gcccgcttcg gccatccgct cgacgatcac
    12061 gtcggccatg tggccgatgt gggcctcgag gcgggcgatc tggcggggtg tgaagccccg
    12121 cgacacgaga ccgcgcaggc gggtgtggtc gggtgggttg acgaagagca tcgagttgcc
    12181 catcacgctg cgttgacggg gcgggaggcc ggggctgaac agcgcggccc ggtcggcgtc
    12241 gaggctgccc ttgcccagcc gcgggtctcg caggacgttg ttgcagtccg accagcgggt
    12301 caggaaccac accgggccga gctcgctgtg atgcaccggc gcggcgtcgc gcagcagccg
    12361 gtaacgggga tagggatcgg cgcggccctc cggggtgagg agggtctcca tgaccagccc
    12421 gtcggggctc atcgggggag gttcggcgct cagtgtgtcg gtcatggcgg tccttcaggt
    12481 cgacgcgtag cgggtcgggg atcccaggtc gatcaccacg tcgccgtcgg cgacgagcag
    12541 atcgacgtgg ccgagcacct cggagatggc gagcaggatc tcgaagccgt cgatgtgggg
    12601 gaacaccgcc cgcgacagct cgtaggcggt cggttcgacc agccgggaga tgtgggcgtg
    12661 gatctcctcg gcccggcgct cgtggtgggc tcgcgtggcg gtcacgagcg cacccaccga
    12721 cgtgaaggcc ggaccgtgcc ccgggagcac gacctgggga tcgagggctt cgaaccggtc
    12781 gagggagccg aggtagtcga cgaggctgcg gcgccgcagc aggtcgctgt cgccgtcgag
    12841 ctcgacgagg gggttgggag tgatcgccgg caacaggtga tcgcccgaga gcaaccgcga
    12901 cgagacaggc tcccacagcg acaggtggcc ggcggcgtgg cccggcgtga ccagcgcctg
    12961 cagcgtccga ccgccgaggc gcagctcgtc gccgtcgtcc accgcgatga cccgtgactc
    13021 gtcgatgtcg tgaagccact cgcggaccgc gccgtagaag gcgggaaaga ccccgagcat
    13081 ctcctcgggg atcccgaacg agcggatcag cgtgtagagc tggtcgcgat cgcggttctc
    13141 cagaagcttc ggaacctcgg cacgaccggc gtagatcggc gcgcccgccc gctcggcgag
    13201 ccatccggcg gtgccgtagt gatcgggatg cgcgtgcgtc acgacgaccg cgccgacgtc
    13261 ggcggcgtcg aggccgacct tcgccagctc ggcggtcaac gtcgccacgc tgtcggcgta
    13321 gagcatcccc gggtcgacga cggtcaccgg gtcatcgagc aacaggtagc agttcaccgg
    13381 gccgaccggg aacggcgtgg gcagctcgaa gcggcgcacc gactctggga gccggtcgag
    13441 cgtcggcacc aagggttcac gcatccgtcg tcctccgtcc gcacatagtg acggcatcta
    13501 gacatcgtgt ctagaccaag ttggacgcga tgtctagact cgccgtgcgg tggtaagccg
    13561 ccctcggagg tctccacgtg acgtctgctc tccatcgcct gggccgctgg tgcgcgcacc
    13621 ggccctggcg cgtcatcgcg ctgtggatgc tggccgccgc cgcggtgctc actgccggcg
    13681 cacgatgggg tgcggcgtcg agtgagagca tcgagatccc cggcaccgaa tcgcaacggg
    13741 cgatcgacct gctcgaggag cgctaccccg acgcagccgg cgggcgcgct cgggtggtct
    13801 tcgccaccga cgccggccgg gtcaccgacc cgcagttccg gtcaggcgcc cagggcaccc
    13861 tggaccagat ccgggccctg ccccgggtcc ggcaggtgat cgaccccttc ggccccgccg
    13921 gcgggttcct cgtctcgccg gacgggcgga tcgcgttcgc cgagatcgtg tacgccgtga
    13981 ccgcccgcga ggtcggcgtc gacggcgtcg atgccctcac cgccaccgcc ggtcccgccc
    14041 gcgacgccgg tctcgatgtt gcgttcggcg gccccgtcgt cgagcggatc ggacccgacg
    14101 acacccacgc ctccgagctc atcggactcg cagtcgcggt gatcgtcctc gccgtcgcgt
    14161 tcggatcggt cacggcgatg gcgttgccga tgtgcacgtc actcgtcggc ctgggcgtcg
    14221 gtctcgcgct catgcgcctc ctcggcgggt tcgtcgaggt cacctccgtc gcgccgatcc
    14281 tcggatcgat gatcggcatc gcggtcggga tcgactactc gctgttcatc gtcacccgac
    14341 accgacagca actcgctcag ggatacgagc tcgacgagtc cgtcgcgcga gccctcgcca
    14401 cctccggcca agccgtcgtg ttcgccggcg tgacggtcat gatcgccatc ttgggcctcg
    14461 cgctcatcgg catccccctt gtcgccgcgc tcggctacgc cgtctcactc atggtcgcga
    14521 tcgcggtcct cgtcgccatc accctgctcc ccgcgctgct cggcctcgtc ggaacccgca
    14581 tcgaccggct ccgcgtccct ggcgtgcaac tgcgctacga gagcgaccac cacacctccc
    14641 ggtccacctc ggcccgatgg gcccgcctcg tcacccgccg accctggccg ttcctcgcag
    14701 ccggctccgt gctcctcgtc gcactggcgg ttcccgcggc ccgcctcgag ctgggctggc
    14761 ccgacgcggg gaacctgccc cctgacacca ccgcccgcca cgcgtatgac ctgctcgccg
    14821 agggcttcgg tgaagggttc aacgggcccc tgctgatcgc cgtcgacctg cgtgaggtcg
    14881 ccgatccccc cggcgcgctg gcaaccatca gcaccgcagt cgccgcaacc ccgggcgtgc
    14941 agttcgcgtc acccgcccag accaacgaca ccggcgacac cgcggtcatc caggtcgttc
    15001 ccaccaccag cccccaagac ccagccaccg aggacctcgt cgaccgcctc cgcggcgaaa
    15061 cggtccccac cctcgaggca gacacccgca ccgtcatcga ggtcaccggc acgaccgccg
    15121 cgttcatcga catctccaaa cgcttggccg accggctcgc cgtcttcatc ggagcggtgg
    15181 tcctgctgtc gttcctcctc ctcaccgcgg tgttccgctc accgctggtc gcgctcaagg
    15241 ccgccctcgt caacctgctg gggatcgccg ctgcctacgg tgcgctcgtc gcggtcttcc
    15301 agtggggatg gggcctcgac gccgtcggcc tcgacgagac cgtgcccatc gtgtcgttcc
    15361 tgccgatgat gatgttcgcc atcctcttcg gcctctccat ggactacgag gtcttcatcc
    15421 tctcccgtgt ccgcgaggag tgggtcgcca ccggcgaccc caccggaagc gtggtcggag
    15481 gcatcgccac ctcggcccgc gtgatcaccg ccgccgcact catcatgatc agcgtcttcg
    15541 catccttcgt tctcggcgac acccccgaga tcaagatgtt cggcttcggc ctcgccgtcg
    15601 ccgtcgccct cgacgccacc gtcatccgca tggtcgtcgt ccccgccacc atgacactgc
    15661 tcggctctcg caactggtgg ctgcccggat ggctcgaccg gatcctgccg aacctcgaca
    15721 tcgagggcac cggcacactc ggcccgaacc ccgccgttgc gggacgcgac gccgctcacc
    15781 ccgacc
//
LOCUS       DAOQOD010000066        15576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1349_length_15576_cov_11.316603, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000066 DAOQOD010000000
VERSION     DAOQOD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_15576_cov_11.316603"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PLZ72_08175"
     CDS             <1..1497
                     /locus_tag="PLZ72_08175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA36309.1"
                     /translation="PGVVVALSGHGDEPVSAERWARRVAPPGWDVVVPEAPTDPDGTR
                     SWFPTGPRGADPVSLERSVGQVAERVLRLQADGRPVAVVGFSQGGALAMCVGLLGCRP
                     DAVVSICGFLPDVDDDSFVERLDRGPDAPPLLVLAGSDDEVTPAFLSEDAAAILGGGG
                     RRVTVSVEPGGHEVSDVAAQRTRSFITDECDRGVKVTLGLPVERTSTGAELVSGDAIV
                     ELAAGYERLGFDAAFVTDHPAPDDRWLAGGGHQAMEPTVALTAAAVATRRLLVHTNVY
                     VLPYRNPFLAAKALASLDVVSGGRLIVGVAAGYLEPEFRALGAGFEDRTDRLEDALEA
                     LPRIWSESKLALSGPGYESRSVTAEPRPLQRPHPPIWVGGNSPAALRRAVDRAQGWSP
                     FPTPGGMESYVRTSAIADLDGLARALERARERCESVGRTEPLTICFTPFALPRYLADP
                     VAGLSELVDEVATLAAMGVDWVSLQVPGLGRSEVLDRAAALAAALGKG"
     gene            complement(1546..2166)
                     /locus_tag="PLZ72_08180"
     CDS             complement(1546..2166)
                     /locus_tag="PLZ72_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNA36310.1"
                     /translation="MSADPSNPTRPVLDRGTAVVTMELERGVVGDLATLDALAAEAAA
                     RGTLAACGRLVGAARSRDVPVVHCVAQWRPDRRGTALNTPLTRALARNPEQILAGSPA
                     VELVAELGDTSGDLTSVRHHGLTPFTGTDLDALLRSLEVSTVVVCGVSLNVGVLGLCL
                     SAADLGYRVVVASDAVVGVPAEYGDSVIAHTLAMVATIVTVSDLLA"
     gene            2202..2927
                     /locus_tag="PLZ72_08185"
     CDS             2202..2927
                     /locus_tag="PLZ72_08185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNA36311.1"
                     /translation="MGLLDADPVAAGSNDAAAAGTAPVDRPIVLASGSRYRAQLLADA
                     GIPVEVMAPDVDERALDHRLDAVGPDGLAVELALLKAAAVAPRCIGRIVVAADQVGVL
                     AEPSGRWRMLTQKSEPAAAVEQLTALSGRRHHLLNGVVVLDEAGDAHIGIDVQVVQMR
                     RFGRTEAESYVGRFEPFDTSGSYRLEDGEAFERDAGPESALVEWVRGEHPSGVLGLPL
                     PLLGRLLDDAHGRGPTHGRPRSG"
     gene            3026..3310
                     /locus_tag="PLZ72_08190"
     CDS             3026..3310
                     /locus_tag="PLZ72_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36312.1"
                     /translation="MRYELIRVAQLVFALATMFGAFLAGLAIGWLRWGRTPPDEPEEA
                     IDLGAVPRIVKTDLFSPAVPPAMVRIDGQLGPAVSAEPVDAEVVAPSAWR"
     gene            3367..3912
                     /locus_tag="PLZ72_08195"
     CDS             3367..3912
                     /locus_tag="PLZ72_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA36313.1"
                     /translation="MDDPGIRRSPAPPASDGLTPEVLVEIERISRLKYAYLRCLDQKD
                     WDGLTDLFTPDAVAEYSGGNYTYAGRDEIVAFIARNMSRETFHSSHRVHQPEIDVDGD
                     DAVGVWALEDTVVDTEWGIVLFGAAFYRDDYRRIDGRWLISRTAYRRSFEAMVPTSSV
                     EGFAVTASWWATDGRSTLPVQ"
     gene            4025..5257
                     /locus_tag="PLZ72_08200"
     CDS             4025..5257
                     /locus_tag="PLZ72_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA36314.1"
                     /translation="MTTVEGSTANTAETGEIPMIVSVDDHVVEPPHVWETWLPAKFKD
                     RGPKIERRGVGAMKHIGGGAYEQEFDPDGKQADCWVFEDLVYIHKRHVAAVGYSRDEM
                     TMTPMTYDEMRPGCYDPKARVEDMLMNHVDVSLCFPSFPRFCGQAFTEAKDRELGEAC
                     VYAYNDWMVEEWCGDSDGHLEPLTIVPLWDADLAAAEVRRNAARGVHAVAFSEVPYHL
                     GLPSIHSGFWDPFFRACEETDTVICMHIGSSSRMPATSPDAPTAVAASLSFNNSMASL
                     SDFLFSGVLVRFPNLKLAYSEGQIGWLPYMLERVDDVWSEHRAWGGVADLIPEPPSSY
                     YYRQVFGCFFRDQHGLNSLDVIGEDNVTFESDYPHTDSTWPHTKEVAEKMMAHLTPEV
                     RYKILRGNAIRMLSLDIT"
     gene            5350..5916
                     /locus_tag="PLZ72_08205"
     CDS             5350..5916
                     /locus_tag="PLZ72_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNA36315.1"
                     /translation="MPEQTTRHRLLVSVPDLGDANFDQTVVYVIEHDGDGALGVVLNR
                     PSDTEVVEHLPELRAPVLSPAVFFVGGPVAVGGVLALGRRRLGSGSDPTGPVAPLPGS
                     LALIDMEALLDGRVDGIDAVRLFTGYSGWAAGQLDAEIAAGAWHVVTALPDDVLCGDP
                     GSLWRSVMRRQGGRTAAQGLFPEDPSVN"
     gene            5994..7004
                     /locus_tag="PLZ72_08210"
     CDS             5994..7004
                     /locus_tag="PLZ72_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36316.1"
                     /translation="MGAATEHHDDVTRIRPRRIRFLTAAVMVSVIVVAAACAPPPPPN
                     QVANPDRTQVVAETHGADRGADLLDVPYLEDAYLGCGAGSASHDPCNGSQTLDVRLHT
                     GDGPARGTIVFVHGGGFTGGDKSDPTGLSGPILAQLGRGWDVVSVNYRLNRGDLAPWP
                     AAVDDVAAAVAWVRGHGAEVGLGTTRIVTAGHSAGGTLATLVALAANSDDPVYAGSDA
                     VDGWIDIAGINDFSTVHGGFWGSRWSAELDQLSARMSPIRFVDAQDPPGYVIHGDDDG
                     LVPASHSLALKDEADRRGARVSLDVVDAWSDRAWMPDRFRNHVPTGGVNVDALNRFLD
                     GI"
     gene            complement(7040..8461)
                     /locus_tag="PLZ72_08215"
     CDS             complement(7040..8461)
                     /locus_tag="PLZ72_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA36317.1"
                     /translation="MTDITPALIDGTDVLTAEVNEVVSPYDGRPVGAVPACETEHLDR
                     AIAVALERHRGGPPPAHERAAVLDRAAQLLSERLDEFAQSISEESAKPITTARVETQR
                     AVDTIRFSAAEARTFTGEMVPLDASSAGVGKLGFTKRVPIGVVGAISPFNFPLNLVCH
                     KIAPAVAAGCPVVLKPASSTPLTALRVARLFEEAGLPPGWINVVTCSGRVANHMVLHP
                     DVAMITFTGSPEVGWGIRADAPRKRVSLELGNNAPVIIEPDGDWEAAATKIAVGGYAY
                     SGQTCISVQRVYVHRSLHEGFVAALADRVAQLRVGPPSDPATEVSALIDPGESDRVSG
                     WISEAAGDGARVVVGGTRGEQGVLVPTLLDGVRPDMKVCHTEVFGPVVGVAAYDGFAE
                     ALALANDTRYGLQAAVFTRDIGAALKAADALDFGGVMVNEIPSWRADQQPYGGVRDSG
                     NTREGPPYTVQEMTERRMIVIQG"
     gene            complement(8567..9421)
                     /locus_tag="PLZ72_08220"
     CDS             complement(8567..9421)
                     /locus_tag="PLZ72_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36318.1"
                     /translation="MVRRRPDERSLVPIIDRMVDQRYAMAVEVVSRLRAEDPSATADE
                     LADRLIRRCAKDLAVGGAVAGGAAASPVAGVAVAAATAGADATWSMGRLSEMIMGIGL
                     IYGHPQHTVDERTALVLAVLGLSESAAVGLTGVAARVGSRGGARLVSRLPSGAAAPST
                     AGATRRTLARLSSSKGPWSLAALVPYGLGAGIGAAGNAVLAHSVGRAARQYFSSTMSP
                     APPRHDPVREDVGEGEVLDEPVGSGPVGSGTASGTASGIVDAEIVDAVIIETVRVRST
                     GDHPPAPG"
     gene            complement(9539..9766)
                     /locus_tag="PLZ72_08225"
     CDS             complement(9539..9766)
                     /locus_tag="PLZ72_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36319.1"
                     /translation="MRRTVNQSLHVATIDGELDDLVLEVPHATHGYTDEHDAPESNEV
                     QRPRKRVRHWKQTFWKRRTNVRRRRAEAYLT"
     gene            9986..10774
                     /locus_tag="PLZ72_08230"
     CDS             9986..10774
                     /locus_tag="PLZ72_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36320.1"
                     /translation="MGDPTVPDSDVGASAEPDQDPDPVLRRVSDLVADRNRARTARPV
                     VVGIGGPVCVGKSTYSAALAERLRGQGGLVTIVVAGDGFLWPNAVLAERGILERKGFP
                     ESYDLLGASGFLDAVRSGDRDVVAPRYSHEDYDVVEGSEPVGAVDVVIFEGVVVLQET
                     FGPRLDLGVYLHAEADLLRQWFVDRFVELVAGLDEGSTSVLGLFRGLDETGLRQAAVG
                     VWESYNVPNNRDHIEPMRDGADIVVELGPSHEILQWHQPRRPRP"
     gene            10947..11138
                     /locus_tag="PLZ72_08235"
     CDS             10947..11138
                     /locus_tag="PLZ72_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36321.1"
                     /translation="MSWGEDRLRDLLGRLDDQYVVLASVRDVVDEMERDVEDGRDWDT
                     DTRDELVRRLRALLLSDEA"
     gene            complement(11444..11899)
                     /locus_tag="PLZ72_08240"
     CDS             complement(11444..11899)
                     /locus_tag="PLZ72_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNA36322.1"
                     /translation="MKILVATDGSPHAIDAAARGLAVLGAAESVTLLCVVHTPAVVSS
                     GMESGFAGGMATPEVVDAAWVGAKEEADASIDRTIAALPEGPAYERRVETGEPGLAIV
                     AAAEEIGADVIVIGSRGRGAIKRALLGSVSSHVVHNASCPVVVTQTHDA"
     gene            12171..13178
                     /locus_tag="PLZ72_08245"
     CDS             12171..13178
                     /locus_tag="PLZ72_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36323.1"
                     /translation="MLGGAELIHLGGGESLPCPGIDSLPLSPEVRNMGLGTSRLALPP
                     ARLHVIPDAVVMPGSGVVCTRDGRVVAESVTSPMIGRVPLDGKELRTPPLDLDGTVAV
                     FRSPLRSDYHTLVDDLPRAGLVAHPALGRLGPITLLHGGALSEMEATLLGSLGGHRVR
                     LREVEPGQPVRAARVVIPNFVTRPGAGAVPSWYRRWSDRVPLPEASDAPRRVYVSAAT
                     DADVPDREELQQVLDLHGIVDLDRVGGSVAERLAVLRDAEMIVGRGRDALAVALISRS
                     AHVVELFDPPTLDPSMYYLAVSKGLPYHYVPVAEDARTALDVPSEGSDALDRVLDRLV
                     A"
     gene            complement(13239..14357)
                     /locus_tag="PLZ72_08250"
     CDS             complement(13239..14357)
                     /locus_tag="PLZ72_08250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36324.1"
                     /translation="MDFTFSEEQQAIGDLAGQVLGDRSTHERLRELERSDGPRFDADL
                     WAELAELGVLGATVPEEHGGAGLDLVALGAVLEAAGRTAAAVPLWETLGLGVPAIARF
                     APASLAAEVLAAVAAGTTVLTAAWHETGAEPLVPTTIASRSGDGWSVTGTKVCVPAGA
                     IADAVLVPAALEESGSVGLFLVRSDRGRADGVGMEPLVTTLGDPQAALLFAEAPATLV
                     AEGAEALRWAYERAVATQCAVATGVCAEALRLTADYTKERKQFDVPIASFQAVAHRAA
                     DAYIDTEAVRLTTHQALWRLGNDQPATAEVATAKFWAAWGGQRVVHAAQHLHGGVGVD
                     RDYPLHRCFLQAKELELQLGGSTQQLLALGDFIAHEPV"
     gene            complement(14390..>15576)
                     /locus_tag="PLZ72_08255"
     CDS             complement(14390..>15576)
                     /locus_tag="PLZ72_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36325.1"
                     /translation="DPMHIAYTEDQQRLRDELRAYYDDLLTDEVKELLHQEAGVGPTH
                     RRIVKQMATDGWLGIGWPTEYGGQGRSQMEQFIFFDESMRMGAPVPMLTINTVGPTIM
                     QYGTDEQKDFFLPKILAGDLHFCIGYSEPGAGTDLAALRTKAVRDGDEFIINGQKMWT
                     SLASDADYCWLAVRTDPDAPKHKGISMIIVDMRSPGITVDPLHLLSSHDINATYFDDV
                     RARADWLVGGENNGWSLIVNQLNHERVTLCSPGLLEQSYMEVREWAQNTTNADGSRVI
                     DREWVQLNLARVHAGLEFLRLINWKVAWTATEGRLDVADASVIKTFGTEFYLEAFRLL
                     MEILGPSAYLERHSVGSVLKGRLEGNYRSLLILTFGGGTNEIQRDLIGMFGMGLPRAI
                     RS"
BASE COUNT         2146 a         5411 c         5668 g         2351 t
ORIGIN      
        1 cccggtgtgg tcgtcgcgtt gtccggccac ggcgacgagc cggtctcggc cgagaggtgg
       61 gcacgcaggg tcgcccctcc gggctgggac gtggtggtgc ccgaggcgcc gacggatccc
      121 gacgggaccc gaagctggtt cccgaccggg ccccgcgggg ccgatccggt ctcactcgag
      181 cggtcggtcg gccaggtggc cgaacgggtg ctccgactgc aggccgacgg ccgaccggtc
      241 gccgtggtgg gcttctccca gggcggagcg ctggcgatgt gtgtgggcct gctcggctgc
      301 cgtcccgatg cggtggtgtc gatctgcggc ttcctgcccg acgtcgacga cgactcgttc
      361 gtcgaacgcc tcgatcgggg cccggacgcc ccgccgctgt tggtgctggc cggatccgac
      421 gacgaggtca cccccgcctt cctgtccgag gacgccgccg cgatcctcgg cgggggcggg
      481 cgacgagtga ccgtgtcggt ggaacccggc ggccacgagg tctccgacgt ggcggcgcag
      541 cggacccgca gcttcatcac cgacgagtgc gaccgcggcg tgaaggtcac gctcgggctc
      601 ccggtcgaac gcacctcgac cggagcggag ctcgtgtcgg gcgacgccat cgtcgagctc
      661 gccgccggct acgagcgcct cggcttcgac gccgccttcg tgaccgacca cccggcgccg
      721 gacgaccgct ggctcgccgg cgggggccac caggccatgg aaccgaccgt cgcgctgacg
      781 gcggctgcgg tggcgacccg acggctgctg gtgcacacca acgtgtacgt cctgccgtac
      841 cggaacccgt tcctggccgc gaaggccctg gcgtcgctcg acgtcgtctc cggcggccgg
      901 ctcatcgtcg gcgtcgccgc cggctacctc gaacccgagt tccgggcgct cggcgccggt
      961 ttcgaggatc gcaccgaccg cctcgaggac gccctcgagg cgctgccccg catctggtcg
     1021 gagtcgaagc tcgctctcag cggccccggc tacgagtcgc gctcggtgac cgccgagccg
     1081 cgcccgctcc aacggccgca cccgccgatc tgggtgggcg gcaacagccc ggccgcgttg
     1141 cggcgcgcgg tcgaccgggc ccaggggtgg tcgccgttcc cgactccggg gggaatggag
     1201 tcctacgtgc gcaccagcgc catcgccgat ctggacggcc tggcccgtgc cctggagcgg
     1261 gcccgggaac gctgcgagtc ggtcggtcgc accgaaccgc tcaccatctg cttcacgccg
     1321 ttcgccctgc cgcggtacct cgccgacccg gtggccggtc tgtccgaact ggtcgacgag
     1381 gtcgccacgc tcgccgcgat gggcgtcgac tgggtgtcgt tgcaggtgcc gggtctgggc
     1441 cgttccgagg tgctcgaccg tgccgccgcg ttggccgccg ccctcgggaa gggttgagcc
     1501 ggccctcccg ggccacggcc tcatcggcca cggagcagac ctgtctcagg cgagcaggtc
     1561 cgacaccgtg acgatcgtgg ccaccatcgc cagggtgtgg gcgatcaccg agtcgccgta
     1621 ctccgcggga acccccacga ccgcgtcgga ggcgacgacg acccggtagc cgaggtcggc
     1681 cgcggagagg cacaggccga gcaccccgac gttgagcgac acgccgcaca cgaccaccgt
     1741 cgacacctcc agggaccgca ggagcgcgtc gaggtcggtg ccggtgaacg gcgtcaaccc
     1801 gtggtggcgc acgctggtca ggtccccgga cgtgtcgccg agctcggcca ccagctcgac
     1861 cgccggcgag ccggccagga tctgctccgg gttccgggcg agggcccggg tgagcggcgt
     1921 gttgagcgcg gtgccccggc ggtccgggcg ccactgcgcc acgcagtgga cgaccggcac
     1981 gtcgcgggac cgggccgctc cgaccaggcg gccgcacgca gccagggtgc cgcgagcggc
     2041 ggcctcggcg gccagggcgt cgagggtggc caggtcgccg acgacgcccc gctccagctc
     2101 catcgtgacg accgcggtcc cgcggtcgag cacaggacgg gtcgggttcg agggatcggc
     2161 ggacacggtc ggtgagcgta ccggtgccac actctcggtc cgtgggactc ctcgacgccg
     2221 atccggtcgc tgcgggatcg aacgatgcgg cggcagcagg caccgcgccc gtcgatcgcc
     2281 cgatcgtgct ggcctccggg tcccgctacc gggcccagct cctggctgac gcgggcatcc
     2341 cggtcgaggt gatggcaccc gatgtggacg aacgtgccct cgatcaccgg ctcgacgcgg
     2401 tgggccccga cggcctggcc gtcgagctcg cgctcctcaa ggccgcggca gttgcccccc
     2461 gatgcatcgg gcgcatcgtc gtggccgccg accaggtcgg ggtgctggcc gagccgtcgg
     2521 ggcggtggcg gatgctcacc cagaagtcgg agcccgccgc ggcggtcgag cagctgacgg
     2581 cgctgtcggg tcggcgacac cacctgctca acggcgtggt cgtcctcgac gaggccggcg
     2641 acgcccacat cggcatcgac gtccaggtgg tgcagatgcg tcgcttcgga cggaccgagg
     2701 cggagtccta cgtcgggcga ttcgagccct tcgacaccag cgggtcctac cgactcgagg
     2761 acggtgaggc gttcgagcgg gatgcggggc ccgaatcggc gctggtggaa tgggtgcgcg
     2821 gcgagcaccc gagcggcgtc ctgggcctgc cgctcccgct cctcggtcga ctcctcgacg
     2881 acgcccacgg tcggggaccc acccacgggc gaccccgctc gggctagggt ccggaccagt
     2941 ttccgtcggg acgtcgtggg gggcacgttc gatccgacgg atggggcagg gaccgttcgg
     3001 gggcggtcgg ggcgaagggg gcgagatgcg ctacgagttg atccgtgtcg cacagctggt
     3061 gttcgcgctg gcgacgatgt tcggggcctt cctggccgga ctggccatcg gctggttgag
     3121 atgggggcgg accccgcccg atgagcccga ggaggcgatc gacctcggcg cggtgccacg
     3181 gatcgtgaag accgacctgt tctcgcccgc ggtccctccg gcgatggtcc ggatcgacgg
     3241 gcagctcgga ccggccgtga gcgcagagcc ggtcgacgcc gaggtggtcg ctccgtcagc
     3301 ctggcgctga cggagcgacc acctcggcgg tcaggatcac cgcaccgaca tcacggagga
     3361 acgacgatgg acgatcccgg catccggcgg tcaccggctc cccccgcctc cgacggcctc
     3421 acgccggagg tcctggtcga gatcgaacgg atctcacggc tcaagtacgc ctacctgcgc
     3481 tgcctggacc agaaggactg ggacggcctg accgatctgt tcacgcccga cgccgtcgcc
     3541 gagtacagcg gcgggaacta cacctacgcg ggccgcgacg agatcgtggc gttcatcgcg
     3601 cggaacatga gccgagagac cttccactcc agccaccggg tgcaccaacc cgagatcgac
     3661 gtcgacggcg acgacgcggt cggggtgtgg gcgctcgagg acacggtggt cgacaccgag
     3721 tggggcatcg tcctgttcgg cgccgccttc taccgcgacg actaccggcg catcgacgga
     3781 cgctggctga tctcgcgcac cgcctaccga cgcagcttcg aggcgatggt tccgacctcg
     3841 tcggtggagg gcttcgcggt gaccgccagc tggtgggcca ccgacggtcg cagcaccctg
     3901 ccggtgcagt agcggcgacg ggcggttccg tcccggcccg gtcctgcgat acagtttctg
     3961 acgacccgtc agaaactgat ccgacggctc gtcagcaccc ggcacgaccg ggggaggagg
     4021 caccgtgacc accgtcgagg gcagcaccgc caacaccgcg gagaccggcg agatccccat
     4081 gatcgtcagc gtcgacgacc acgtcgtcga accgcctcac gtgtgggaga cgtggctccc
     4141 ggccaagttc aaggaccggg gtcccaagat cgaacggcgc ggcgtcggcg ccatgaagca
     4201 catcgggggc ggcgcgtacg agcaggagtt cgaccccgac ggcaagcagg ccgactgctg
     4261 ggtcttcgag gacctcgtct acatccacaa gcgccacgtc gccgccgtcg gctactcccg
     4321 cgacgagatg accatgacgc cgatgaccta cgacgagatg cgcccggggt gctacgaccc
     4381 caaggcccgc gtcgaggaca tgctcatgaa ccacgtcgac gtgtcgctgt gcttcccgtc
     4441 gttcccgcgc ttctgcggcc aggcgttcac cgaggccaag gaccgcgagc tcggcgaggc
     4501 ctgcgtgtac gcctacaacg actggatggt cgaggagtgg tgcggtgact ccgacggcca
     4561 cctggaaccc ctgaccatcg ttcccctctg ggacgcggac ctggccgccg ccgaggtgcg
     4621 tcgcaacgcg gcgcgcggcg tgcacgcggt ggcgttctcc gaggtgccgt accacctggg
     4681 tctgccgagc atccacagcg gcttctggga cccgttcttc cgggcgtgcg aggagaccga
     4741 cacggtcatc tgcatgcaca tcggatcgtc gtcgcgcatg cccgccacct cgccggacgc
     4801 tccgaccgcg gtggcggcgt cgctcagctt caacaactcg atggcgtcgc tgtcggactt
     4861 cctgttctcc ggggtcctcg tgcgcttccc gaacctgaag ctggcctact ccgagggtca
     4921 gatcggttgg ctgccctaca tgctcgagcg cgtcgacgac gtctggagcg agcaccgggc
     4981 ctggggcggt gtcgccgacc tcatcccgga gccgccgtcg agctactact accggcaggt
     5041 gttcggctgc ttcttccggg accagcacgg tctgaactcc ctcgacgtca tcggagagga
     5101 caacgtcacc ttcgagagcg actaccccca caccgactcg acctggcccc acaccaagga
     5161 ggtcgcggag aagatgatgg cgcacctcac ccccgaggtc cgctacaaga tcctgcgggg
     5221 caacgccatc cggatgttgt cgctcgacat cacctgatcg tcggttcgtt cccagagcct
     5281 gttcccggcc ccggccccgc gatcgtgtcc gcggtgccgg ggccggggcg cgtctgggaa
     5341 gctggggtgg tgccggagca gaccacgcga caccgcctcc tcgtgtcggt acccgacctg
     5401 ggcgacgcca acttcgacca gacggtggtc tacgtgatcg agcacgacgg tgacggggcg
     5461 ctgggcgtgg tgctgaaccg cccgtcggac accgaggtcg tcgagcacct gcccgagctg
     5521 cgcgccccgg tcctgtcgcc cgcggtgttc ttcgtcggtg gcccggtggc ggtcggtggt
     5581 gtgctcgcac tcgggcggcg tcgtctgggc agcgggagcg accccaccgg cccggtggcc
     5641 cccctcccgg gatctctcgc gctgatcgac atggaggcgc tgctcgacgg acgggtggac
     5701 ggcatcgacg cggtgcggtt gttcaccggc tactccggct gggccgctgg tcagctcgac
     5761 gcagagatcg cagccggggc gtggcacgtc gtcaccgccc tgcccgacga cgtgctgtgc
     5821 ggtgatcccg gttcgctgtg gcgttcggtg atgcggcgtc agggtggccg gacggcggcc
     5881 cagggcctgt tccccgagga cccgtcggtc aactgacccg attccgctcc ggcgcgtggg
     5941 tcgaacggac cgatgttctc aaccggtgac aggagggagc cgttgccccg gacatgggag
     6001 cagcaaccga gcaccatgac gacgtcacgc ggatccgccc acggcgaatc cggttcctga
     6061 ccgccgccgt gatggtgtcg gtgatcgtgg tcgccgctgc gtgtgcgccg ccgccgccgc
     6121 cgaaccaggt cgccaacccg gatcgtacgc aggtggtcgc cgagacccat ggcgccgatc
     6181 gcggtgcgga cctgctcgac gtgccctacc tcgaggacgc ctacctcggc tgtggtgcgg
     6241 gttcggcctc gcacgatccg tgcaacggat cccagacgct cgacgtccga ctccacacgg
     6301 gcgacggtcc ggcgcgaggg accatcgtct tcgtccacgg cggcggcttc accggcggtg
     6361 acaagagcga tccgacgggg ctcagcggtc cgatcctggc ccagctcgga cggggttggg
     6421 acgtcgtgtc ggtcaactac cggctgaacc ggggggacct cgcgccctgg ccggccgcgg
     6481 tggacgacgt cgcggcggcg gtcgcctggg tccgaggaca cggcgcggag gtcggtctgg
     6541 ggacgacccg catcgtcacc gccggccact ccgcgggcgg gacgctggcc acgctcgtcg
     6601 ccctggccgc gaactccgac gacccggtct acgccggatc ggacgcggtg gacgggtgga
     6661 tcgacatcgc cgggatcaac gacttctcga cggtccacgg ggggttctgg ggttcccgct
     6721 ggagcgcgga gctggatcag ctctcggccc gcatgtcgcc gatcaggttc gtggacgcgc
     6781 aggacccgcc cggctacgtg atccacggcg acgacgacgg cctcgtgccg gcttcccact
     6841 ccctcgcact caaggacgag gccgatcggc gtggcgcccg ggtcagcctg gatgtggtgg
     6901 atgcctggag cgaccgtgcg tggatgcccg accggttccg caaccacgtc ccgaccggcg
     6961 gggtcaacgt cgacgcgctg aaccggttcc tcgacgggat ctgatcagtc cgcggagtcg
     7021 ttccggcccg ccgtgtcgtt cagccctgga tgacgatcat gcggcgctcc gtcatctcct
     7081 ggacagtgta ggggggacct tcccgggtgt ttcccgagtc gcgcacgccc ccgtagggct
     7141 gctggtcggc ccgccacgac gggatctcgt tgaccatcac acccccgaag tcgagcgcgt
     7201 cggcggcctt gagggcggca ccgatgtctc gggtgaacac ggcggcctgc aggccgtagc
     7261 gggtgtcgtt ggccagggcg agggcctcgg cgaagccgtc gtaggcggcg acgccgacga
     7321 cggggccgaa cacctcggtg tggcacacct tcatgtccgg gcgcacgccg tcgagcaggg
     7381 tggggaccag cacgccctgc tcgccgcggg tcccgccgac cacgacccgg gcgccgtcgc
     7441 cggccgcctc ggagatccag ccgctgaccc gatccgactc gcccgggtcg atgagggcgc
     7501 tgacctcggt ggcaggatcg gacggaggac cgacgcgcag ttgcgccacc cggtcggcca
     7561 gagcagcgac gaatccctcg tggaggcttc ggtgcacgta cacccgttgc accgagatgc
     7621 aggtctggcc ggagtacgcg tagccgccga cggcgatctt ggtcgcggcg gcctcccagt
     7681 cgccgtccgg ctcgatgatg acaggggcgt tgttgccgag ctccaggctc acccgcttgc
     7741 gaggggcgtc ggctcggatg ccccagccga cctcgggtga tccggtgaag gtgatcatgg
     7801 cgacgtcggg atgcagcacc atgtggttgg cgacccggcc gctgcaggtc acgacgttga
     7861 tccacccggg cggaaggccg gcctcttcga agagccgggc gacgcgcagc gcggtgagcg
     7921 gggtgctcga cgcgggcttg aggacgaccg gacaaccggc agcgaccgcc ggagcgatct
     7981 tgtggcacac caggttgagc gggaagttga acggcgagat cgcaccgacg acaccgatcg
     8041 ggacccgctt ggtgaagccc aacttgccga caccggccga cgaggcgtcg agggggacca
     8101 tctccccggt gaacgtccgg gcctccgcgg cggagaagcg gatcgtgtcg accgcccgct
     8161 gcgtctccac ccgggcggtg gtgatcggct tggcggactc ctccgagatc gactgggcga
     8221 actcgtcgag ccgctccgac agcagctgcg cggcgcggtc caggacggca gcccgctcgt
     8281 gggcgggagg gggaccgccc cggtggcgct cgagcgccac cgcgatggct cggtcgaggt
     8341 gctcggtttc gcaggcgggg acggcaccga ccggacgacc gtcgtagggg gagacgacct
     8401 cgttgacctc tgcggtcagg acgtcggttc cgtcgatcaa cgccggggtg atgtcggtca
     8461 tggcccgagg ctacccacgg ccccgctccc gagaacggcc gttgcagccg cggtgctcgg
     8521 cactaccgct cggcgcgtcc gcggtgctcg gcgatcggtg agggagtcag cccggcgccg
     8581 gggggtgatc gccggtggaa cggacccgca ccgtctcgat gatgaccgcg tcgacgatct
     8641 cggcgtcgac gatcccggaa gccgtcccgg aagccgttcc ggaaccgaca ggtccggaac
     8701 cgacgggctc gtcgaggacc tccccctctc cgacgtcctc gcgcaccggg tcgtggcgcg
     8761 ggggagcggg gctcatcgtc gacgagaagt actggcgggc cgcccggccg acagagtggg
     8821 ccagcaccgc gttgccggcc gctccgatgc ccgcacccag cccgtacggc acgagcgcgg
     8881 ccaggctcca cggccccttg gagctcgaca acctcgcgag cgtccgccgc gtggctccgg
     8941 cggtggaggg cgcggcggcg cccgagggca ggcgtgacac gaggcgggcg ccgccccggg
     9001 agccgacccg tgccgccacc ccggtcagtc cgaccgcagc gctctcggac aggccgagca
     9061 cggcgagcac gagtgccgtg cgctcgtcga cggtgtgctg ggggtggccg tagatgaggc
     9121 cgatgcccat gatcatctcc gacaggcggc ccatcgacca ggtggcatcg gcccctgcgg
     9181 tggccgcggc gaccgccacg cccgcgaccg gtgaggcggc ggccccgcca gcgaccgctc
     9241 cacccaccgc gagatccttg gcacagcgac ggatcagccg gtccgcgagc tcgtcggcgg
     9301 tggccgaggg gtcctcggcg cgcaggcgtg acacgacctc gacggccatg gcgtatcgct
     9361 ggtcgaccat ccggtcgatg atcgggacga gcgagcgctc gtcggggcga cgacggacca
     9421 cgtcgtcacg ctaacggagc atgccggttc ccggctgtcg tctcgaccgc cggtgcgttg
     9481 atccgatcac cgctccaccc gcgtgccgga gcatcggcgg gtggagcagc acgtccgctc
     9541 aggtgaggta ggcctcggcc cgccgcctcc gcacgttcgt ccgccgcttc cagaaggtct
     9601 gcttccagtg ccgcacccgc ttgcggggcc gctgcacctc gttggactcg ggggcgtcgt
     9661 gctcgtcggt gtagccgtgg gtggcgtgcg gaacctccag gaccaggtca tcgagctccc
     9721 cgtcgatggt ggcgacgtgg agactctggt tgaccgtgcg tcgcacccgt cggtgctccg
     9781 cctttcggag atctcgctcc tcctgcagcg cagaagttgc tccggaccga cgagtgcggt
     9841 ggcggttgga catcggcacc tccctggatc ttgtgtggcc tgggttgggc tgcgcgaacc
     9901 ggacgggtcc ggcccgcacc cctaggggac accccgggcc gaccgatgtc aagcatcccc
     9961 cgggtgatcg tgacatggtg gggacatggg tgatccgacg gtgcccgact ccgacgtcgg
    10021 ggccagcgcc gagcccgatc aggatccaga cccggtgctc cgccgcgtga gcgacctcgt
    10081 cgccgatcgg aaccgtgcca ggacggctcg accggtggtg gtcggcatcg gaggcccggt
    10141 gtgcgtcggc aagtccacgt actccgccgc gctcgccgag cgccttcgtg gccagggtgg
    10201 tctcgtgacc atcgtggtcg cgggcgacgg gttcctgtgg cccaacgcgg tgctggctga
    10261 gcgggggatc ctcgagcgca agggcttccc cgagtcatat gacctgctcg gagccagcgg
    10321 gttcctcgac gcggtgcgat ccggcgatcg cgatgtggtg gcaccgaggt actcccacga
    10381 ggactacgac gtcgtggagg ggagcgaacc ggtcggcgcg gtcgacgtgg tgatcttcga
    10441 gggagtggtc gtcctgcagg agaccttcgg gccgcgtctg gacctcgggg tctacctgca
    10501 tgccgaggcc gacctcctga ggcagtggtt cgtggatcgc ttcgtcgagt tggtcgccgg
    10561 tctcgacgag ggttcgacct cggtgctcgg gttgttccgt ggcctggacg agacaggact
    10621 tcggcaggcc gcggtggggg tgtgggagtc ctacaacgtc ccgaacaacc gcgaccacat
    10681 cgagccgatg cgcgacggag ccgacatcgt ggtcgagctc ggtccgtccc acgagatcct
    10741 gcagtggcac cagccgcgcc gcccgcggcc ctagattcgt cactgtgacg gaactgcggg
    10801 gcgggggacc gggggcgggg gacgacggcc gctgctcgtg gtgtggacgc cggctgcccg
    10861 atccggccgg aaccggccgc ccacggaggt actgccggcg gagctgtcgt cagagggcct
    10921 tcgaggcccg ccggcggctg gacgagatgt cctggggcga agatcgtctg agggatctgc
    10981 tcgggcgact cgatgaccag tacgtggttc tggccagcgt gcgtgacgtc gtcgacgaga
    11041 tggagcgtga cgtcgaggac ggccgggact gggacaccga cacccgggac gagctcgtcc
    11101 gtcgcctccg ggcgttgctg ttgtccgatg aggcctgacc cgtcgtggcg tgcaacgggc
    11161 gtgcgtcccg acgggatgcc ctctcgcaag acgccggaaa tgctgattat gtccgtaggc
    11221 cgggacggcc gtcgcgcctc tgtccgaact tgtagctggc aggtcgcctg acggtcggtg
    11281 gactacaagt ttgccggcgg gcaccgaact tgtagctggc aggtcgcctg acggtcggtg
    11341 gactacaagt tcgccggcgg gcaccgaact tgtagctggc aggtcgcagg acggtcggtg
    11401 gactacaagt tcgccggcgg gcaccgaacc aggcggtggg ctgtcaggcg tcgtgggtct
    11461 gggtcacgac caccgggcac gaggcgttgt gcaccacgtg gctgctcacc gagccgagca
    11521 gggcgcgctt gatcgcccct ctcccccgcg acccgatcac gatgacgtcg gccccgatct
    11581 cctccgcggc ggcgacgatg gcgaggccgg gctcgcccgt ctcgacgcgt cgttcgtagg
    11641 ccggtccctc gggcagggcc gcgatcgtgc ggtcgatgct ggcgtcggcc tcctccttgg
    11701 cgcccaccca cgcggcgtcc accacctcgg gcgtggccat gccgccggcg aagccggact
    11761 ccatgcccga cgacacgacc gcgggcgtgt gcacgacgca cagcagggtg accgactcgg
    11821 ccgcaccgag gacggccagc ccccttgccg cggcgtcgat ggcgtgcgga gagccatcgg
    11881 tggcgacgag gatcttcacg ggtgggtccc cttccggtcg gcctgtcgtc agggcgacga
    11941 tcgctctgac gacgtgaggg taccggaggg tcggaggggt cgtcgcgcct cggtccgggg
    12001 gggtcggaat atccccgagg tcggtcatcc atcgctgggc tcagttcggt tacagttccg
    12061 tgacgatggg aaggatggtg gagctgatga cgaggtggtt gcctccctcc ggtcgtggcc
    12121 ggctcagcgc tcgccgtggt catgcctggc cccgtaccgt cccggcgtcg atgctgggcg
    12181 gcgccgagct catccacctc ggtggtggcg agtcgctgcc gtgcccgggc atcgactcgc
    12241 tccctctgtc gcccgaggtc cgcaacatgg gactgggcac atcgaggctt gcgctgcccc
    12301 ccgctcggtt gcacgtgatc cccgacgccg ttgtgatgcc gggcagcggt gtggtctgca
    12361 cccgtgacgg ccgggtggtc gccgagagcg tcacctcgcc gatgatcggg cgagttccgc
    12421 tcgacggcaa ggagttgcga acccctccgc tcgatctcga cggcacggtg gcggtgttcc
    12481 gatctcccct gcgctccgac taccacacgc tggtcgacga ccttccccgg gcggggctgg
    12541 tggcccatcc cgcgctcggg cggctcggtc cgatcacctt gctccacggt ggggccctga
    12601 gtgagatgga ggcgaccctg ttggggagcc tcggagggca ccgggtcaga ctccgggagg
    12661 tcgaacccgg tcagccggtc cgggcggccc gggtcgtcat ccccaacttc gtgacccgtc
    12721 cgggcgcggg tgctgtgccg agctggtatc gccgatggag cgatcgcgta cccctgcccg
    12781 aagcatccga tgcacctcga cgcgtgtacg tctcggctgc gaccgacgcc gacgtgcccg
    12841 accgagagga gctgcagcag gtcctcgacc tccacggcat cgtcgatctg gaccgggtcg
    12901 gggggtcggt ggccgaacgg ctggcggtcc tgcgcgatgc ggagatgatc gtcggtcggg
    12961 gtcgcgacgc cctggccgtg gcgctgatca gccggtccgc ccacgtggtc gagctgttcg
    13021 acccgccgac cctcgatccg tcgatgtact acctcgcggt gtccaagggc ctgccctacc
    13081 actacgtccc ggtggccgag gacgcccgga cggctctcga tgtcccatcg gaggggtccg
    13141 acgccctcga ccgggttctc gaccggctgg ttgcctgagt cggcacggga gcaggacccg
    13201 cggcgggcag cgccgatcgt cgggaacccc ggcggaattc agaccggttc gtgggcgatg
    13261 aagtcgccca acgccagcag ctgctgcgtg gacccgccga gctgcagctc cagctccttg
    13321 gcctgcagga agcaccggtg cagcgggtag tcgcggtcca cgcccacgcc gccgtggagg
    13381 tgttgggccg cgtgcacgac ccgctgacca ccccaggcgg cccagaactt ggcggtggcg
    13441 acctcggcgg tggccggctg gtcgttgccc aggcgccaca gcgcctggtg cgtggtcagc
    13501 cgcaccgcct cggtgtcgat gtaggcgtcg gccgcccggt gagccacggc ctggaacgac
    13561 gcgatcggca cgtcgaactg cttgcgctcc ttggtgtagt cggcggtcag gcgcagcgcc
    13621 tcggcgcaca ctccggtggc gaccgcgcac tgggtggcca cggcccgctc gtaggcccag
    13681 cgcagggcct cggccccctc ggcgacgagc gtggccggtg cctcggcgaa cagcagggcg
    13741 gcctgggggt cgccgagagt ggtgacgaga ggctccatcc ccacgccgtc ggcacggccc
    13801 cgatccgagc gcaccaggaa gaggccgacc gagccggact cctcgagcgc ggcgggaacc
    13861 agcaccgcgt cggcgatcgc gccggcgggg acgcagacct tggtgcccgt cacgctccag
    13921 ccgtcaccgg agcgtgacgc gatcgtggtc ggcaccagcg gctcggcgcc ggtctcgtgc
    13981 cacgccgcgg tcaacaccgt cgtgcccgcg gccactgcag ccagtacctc ggcggccagc
    14041 gaggccgggg cgaagcgggc gatggcgggg acgccgaggc cgagcgtctc ccacagcggg
    14101 actgccgccg cggtccttcc ggcggcttcg agcacagcgc cgagggcgac caggtcgagc
    14161 cccgctcccc cgtgctcctc gggcacggtg gcgccgagaa cgccgagctc cgcgagctcg
    14221 gcccacaggt ccgcgtcgaa tcgcgggccg tcggaccgtt cgagctcgcg caggcgctcg
    14281 tgggtcgagc ggtcgccgag cacctgaccg gcaaggtcgc cgatcgcctg ctgctcctcg
    14341 ctgaaggtga agtccatgtc gtgtcgtcct ctcgtcccgt cccggatgct cagctgcgga
    14401 tcgcccgcgg caggcccatg ccgaacatgc cgatcaggtc ccgttggatc tcgttggtgc
    14461 cgccgccgaa ggtgaggatc agcaggctgc ggtagttgcc ctccaggcgg cccttgagca
    14521 ccgagccgac cgagtggcgc tccaggtagg ccgacgggcc gaggatctcc atcagcaggc
    14581 ggaacgcctc caggtagaac tcggtgccga aggtcttgat gaccgacgcg tcggccacgt
    14641 cgaggcggcc ctcggtggcg gtccaggcga ccttccagtt gatcaggcgg aggaactcga
    14701 gcccggcgtg cacacgggcc aggttgagct gcacccactc acggtcgatc acccgggagc
    14761 cgtcggcgtt ggtggtgttc tgcgcccact cccgcacctc catgtaggac tgctcgagca
    14821 gaccgggcga gcacaacgtc acgcgctcgt ggttgagctg gttgacgatc aggctccagc
    14881 cgttgttctc gccgccgacc agccagtcgg cccgggcccg gacgtcgtcg aagtaggtgg
    14941 cgttgatgtc gtggctggac agcaggtgca acgggtcgac ggtgatgccc ggcgagcgca
    15001 tgtcgacgat gatcatcgag atgcccttgt gcttgggtgc gtcggggtcg gtgcgcaccg
    15061 ccagccagca gtagtccgcg tccgaggcca gcgacgtcca catcttctgg ccgttgatga
    15121 tgaactcgtc gccgtcgcgg acggccttgg tgcgcagggc ggccaggtct gtgcccgcac
    15181 ccggctcgga gtagccgatg cagaagtgca ggtctccggc caggatcttg ggcaggaaga
    15241 agtccttctg ctcatcggtg ccgtactgca tgatcgtcgg cccgaccgtg ttgatggtca
    15301 gcatcgggac cggggcgccc atgcgcatcg actcgtcgaa gaagatgaac tgctccatct
    15361 ggctgcggcc ctggcctccg tactcggtgg gccagccgat gccgagccag ccgtcggtgg
    15421 ccatctgctt gacgatgcga cggtgggtgg ggccgacccc cgcctcctgg tgcaggagct
    15481 ctttgacctc gtcggtcagc aggtcgtcgt agtaggcgcg cagctcgtcg cggaggcgct
    15541 gctggtcctc cgtgtaggcg atgtgcatgg gatctc
//
LOCUS       DAOQOD010000067        15491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1359_length_15491_cov_12.585644, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000067 DAOQOD010000000
VERSION     DAOQOD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15491
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1359_length_15491_cov_12.585644"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLZ72_08260"
     CDS             <1..306
                     /locus_tag="PLZ72_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36326.1"
                     /translation="GGSEIPWSIKNNAIHGNLTVVGQTSEWIGILFNRIDRNVILAGI
                     TLTGTDDSTGTGTNSLFVVRNTIGRNIACGWIDPGVFGYGNSISGHALGLCEPLTAS"
     gene            413..865
                     /locus_tag="PLZ72_08265"
     CDS             413..865
                     /locus_tag="PLZ72_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36327.1"
                     /translation="MELDNPTPIDSQVVRCGPLTDADRDLIAAATEVVDRCSDGANHT
                     VAAAVRDASGRIHTGVNVFHFTGGPCAELVVLGVARAAGAGELESIVAVGDEDRGVLS
                     PCGRCRQVLADLHGGLRVILPTGGALAAVSAVDLLPFRYEQTYERRLR"
     gene            complement(893..2329)
                     /locus_tag="PLZ72_08270"
     CDS             complement(893..2329)
                     /locus_tag="PLZ72_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA36328.1"
                     /translation="MTDHDAAPDADQELPAGPTRSRRLSASDTVLWDLDRDPVLRSTI
                     TAVALLDRAPDWGRLRTRIDRASQLIPRMRQRVGPAPFGIGAPRWVDDGDFDLDHHLR
                     RVRVPLPGRLRDVLDLVQPIASDSFDPVRPLWEFTLVEGMRGGGAALVQKLHHCLTDG
                     VGGMELALLLLDDVRDQPEPDDLSLRLSPHLATPPGAQGLRRAARVPLAVSSAALTAA
                     TATLKDPLGTVTDVGELAGGVLRLLSPVRVTSPLMERRSLRRRFHSIDVSLEDLRAAG
                     HAAGGTLNDAFMAAVVEGLRRYHQDHGTELEHLSITMPVSMRRTGDAVGGNRFTPARF
                     AVPARAMPPIEQMRTIGAIAHSWQHSRALALTDAVAGALDVLPPPVVSAVMGSMLKGI
                     DTVITNVPGLPSGCYLAGSEIVREYAMAPTSGAAVNVALVSVGETACIGVSVDLGAVP
                     DDDVLLEKLVDGFRSITDVVGHQPLRRA"
     gene            complement(2407..3825)
                     /locus_tag="PLZ72_08275"
     CDS             complement(2407..3825)
                     /locus_tag="PLZ72_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA36329.1"
                     /translation="MIGHVGSAAAIETHSASLFFFGDRVYKVKKPVDLGFLDFRTEEG
                     RAAACRREVELNRRLAPDVYLGVAQMTDPDGQVCEHLVVMRRMPPHRRLRACLERGED
                     VHVALERIADELASLHDASPPPGPMESVATRDAIRRLWCDGFDSMRPHVGTLLGATDQ
                     QRIEELVLRFLAGRAPLFEQRIRDGAVRDGHGDLQAEDIFLLDDGPRVLDCLEFDDTL
                     RWGDVLGDAAFLAMDLERFGRPELARLFLDAFGQRSGIPWPPALEHHYVAYRAHVRAK
                     VGVIRAGQGAGSTAEVDRLQALCRRHLEAARVRAVLVGGLPGTGKTTLSRRLAERVDA
                     VVLRSDELRRRHPEAGRYAPTTTDEVYADLLAEAEHHLAHGRHVVLDATWSAPEHRRL
                     ARTVADRVAADVVEIECVAPAGMTVERIRHRLAAGVDLSEATPEVAEQMARRFGPWPE
                     AEQVRTDLAPEDSTRSALLAFG"
     gene            complement(3822..4862)
                     /locus_tag="PLZ72_08280"
     CDS             complement(3822..4862)
                     /locus_tag="PLZ72_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNA36330.1"
                     /translation="MDAVQLTQWKHDPEVRDVPQPDPGPGQVLVRIAGAGMCHSDLHL
                     IHDFESGMVPFEPPFTLGHENAGWVEAVGVGVSGLELGQPVAVYGPTGCGRCHRCVQG
                     MQNYCEHQAELTSMAAGLGDDGGMAQFMLVHDARHLVPLDDLDPVEAAPLTDAGLTPY
                     HAVKRSADLLVAGSTALVIGVGGLGHLGVQILEAVTPATVIAVDTRTSALDLAATAGA
                     EHTVQAGDDAAAEIADLTGGKNADVVLDFVGSDSTIQLAVAAGRSLGHITVVGIAGGS
                     YPFSFFTVPYEASLATTYWGSIPELMEVLNLAERGLIRAEVERISIDEAPDGYRRLAR
                     GEVDGRLVVVPG"
     gene            complement(4980..7301)
                     /gene="ppsA"
                     /locus_tag="PLZ72_08285"
     CDS             complement(4980..7301)
                     /gene="ppsA"
                     /locus_tag="PLZ72_08285"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNA36331.1"
                     /translation="MSATTTDGAIAWLDEVSLAEVDRVGGKGANLGEMASAGFPVPPG
                     FVVCADGYLAAMDAAGVRRELLSEFREAVASADDPVELRARARHLQETVRRAGVPGAL
                     RTALVDAYHRLGDDVAVAVRSSATAEDAAGTSFAGMHSTFTDVAGPDDLIRRLLDCWV
                     SLYGERVISYRAGQSLTDEPSIAVVVQQLVPSERAGVMFSVDPSTGDQDLIVIEAALG
                     LGEVVVSGQVQPDTYTLTKDGPRIREVRVGHQTHRLVASSGGRVDREELGSADADDRV
                     LTDGQAIELAELGLHVEEHYGEPQDMEWAIGEGRTYLVQSRPITTLDTGPSGEPTAGT
                     ADDAATLLQGLAASSGTASGRVRVLMTPDRAADLLSGEVLVAPLTNPDWVPAIRRAAA
                     VVTDSGGMTCHAAIVSRELGVPCVVGARTATTLLRDGEVVTVDGRRGTVLRGAVPVVA
                     PATAVEPRAADAAPLEVPALATRIYVNLAMSEHAVEVAAGPVDGVGLLRAEFMVADAL
                     DGVHPRQLLAQGGRDEFLRRMSDSLLRITRAFTPRPVVYRTIDFRSNEFRGLRGGEQF
                     EPVEENPMIGYRGCFRYVKEPDLFALELELLARVRDETPNLHLMIPFVRTRWELERCL
                     ELVDASPLATHRNLHRWVMAEVPSVVHRIPEYAAMGIDGVSIGSNDLTQLLLGVDRDS
                     TVCAELFDESDAAVLDAIARIIRAAHDSGITASLCGQAPSNDPEFAAHLVRLGIDSIS
                     VNPDAVDAARRAVGAAERRLLVDSARRGRDRTG"
     gene            complement(7402..8409)
                     /locus_tag="PLZ72_08290"
     CDS             complement(7402..8409)
                     /locus_tag="PLZ72_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNA36332.1"
                     /translation="MRAWSVTAPAPIDRGPLELSDRPDPEPGPGEVRVRVSVCGVCRT
                     DLHVAEGDLAVHRAGVVPGHEIVGVVDRLGPGASRFAEGDRIGIAWLRRTCGVCRFCR
                     RGAENLCTSPSFTGWDEDGGYAEHAVVDERFAYSLPAGFADEHVAPLLCAGIIGFRAL
                     RRAELPPGGRLGIYGFGASAHITAQIAIAQGATVHVMTRSPEARELALALGCASVGGA
                     TDAPPEPLDSAINFTPSGDVVPAALRALDRGGTLAVAGIHMSDTPPLDYDRELFQERQ
                     IRSVTANTRRDGEDLLRIAGRIGVQVHTTAYPLERADEALADLAHDRLTGAAVLRVGP
                     P"
     gene            complement(8459..10204)
                     /locus_tag="PLZ72_08295"
     CDS             complement(8459..10204)
                     /locus_tag="PLZ72_08295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNA36333.1"
                     /translation="MTTGSCCTVHPIAAERLGPVLRSTNTTISPANHGRRTSTRSTPQ
                     ADLLVVGHISTGDPSTPSAEAMAVSDGRIVGIGSRSDVDGLTGPSTTIVAPDGVVIPG
                     LVEPHMHIWASLLNLDWLDLSHEACPTFDDVVSALRSAVGGAAPGEPLMAKQFDPSLY
                     PGEPVLTRDLLDRISPDRSVVVVNASLHYMYANSTALAAAGVTDTTPDPPGGTFGRVD
                     GRLNGVIGEVPAMMALMAAVPMPTSDQITDGVRRILDAAARRGVTSLREAATGTLGGV
                     GELALLHQINGSRRLPVRVSTAQFSIMGDRTPSEVAAAWRDADVTPFSGDDMVRADAW
                     KVVTDGSNQGRSGYFLQPYLGEESGGKANWTPEGLRDVLEVGLSDGWQVMVHTNGDAA
                     VEFALCAMEDLLPHHPAGDLRHRFEHVSFSTDDQLTRMAAAGISPSFLMNHVYFWGAA
                     FRDTILGPERAQRLDRVASAYAAGMRPSLHSDYTVTEIHPLLSARTAVQRRLQADGTV
                     LNPAECVSPDQALAAITTHAAWQIHADDRGSLGVGQRADFAVVSDDPWASDPDGWPEI
                     TVSETYLDGTPAYQG"
     gene            complement(10251..10943)
                     /locus_tag="PLZ72_08300"
     CDS             complement(10251..10943)
                     /locus_tag="PLZ72_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36334.1"
                     /translation="MAAPLDRSSARRSGSRASGRSYGGVPAEVRVAQRRDRFLAAGLE
                     LFGTRGYQSTSVRAVCREAGLSERYFYESFANTEALLIGVYQHCTDRMHDDILVAMAT
                     GDRPAGGSSAEPLDHVVRTTLEAFYASVADPRVLRVCWLEVLGVSTLVDSTYISGIDR
                     FAHLVATVMAETFGLSPDERLRLIATGLVGSVIMTAIRWYLDGQKEPVTTLVAANSVL
                     FLGAARQLSPRS"
     gene            11085..11417
                     /locus_tag="PLZ72_08305"
     CDS             11085..11417
                     /locus_tag="PLZ72_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNA36335.1"
                     /translation="MDVHEVIVIGSGFSGQSAVIGLQKAGIEDWIVLERRSFMGGTWC
                     QNTYPGAAVDVQSPLYSISSEPYPWSQIFAERDELERYTNFIIDKHHIRERTELNADV
                     SALDWDDD"
     gene            11427..12545
                     /locus_tag="PLZ72_08310"
     CDS             11427..12545
                     /locus_tag="PLZ72_08310"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA36336.1"
                     /translation="MISTRDGRTFCARFVILGPGPLSNPKIPDFEGRDTFEGHSFHSN
                     AWDHDYDLAGKRVAVIGSGASAAQIIPAIAPEVGELHVFQRTPHWVLPRRDRKFSRLE
                     RALLKNRRVYGALRSAIYLGLETRVIGFKYFPRLLDLVAKRQALAHIARQIPDPEIRR
                     KVTPDYTIGCKRVIVSSTLYPALCRPNVKLHDSSDGIDEIVPAGIITTGGDKVELDAI
                     VYATGYDATDGATTFTVTGHDSTPITDLWAEFPRAYLGTTMPGFPNLFLMLGPNTGIG
                     HTSALFIMESQLRYALDCIQRTRRNNRHAIEPSVEAEDRYTEWVHRAMERTVWKSGGC
                     SSWYQNSSSGKVIAMYPGFSFSYRFRAGRFRSEDHIFT"
     gene            12656..12883
                     /locus_tag="PLZ72_08315"
     CDS             12656..12883
                     /locus_tag="PLZ72_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNA36337.1"
                     /translation="MADTWLQTLITETPQEGFELAITLSRRGVKYTQPDTDVLHELRP
                     DYANSADSLTAASQVVAVNFQTVAAANNYWR"
     gene            complement(12871..13737)
                     /locus_tag="PLZ72_08320"
     CDS             complement(12871..13737)
                     /locus_tag="PLZ72_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36338.1"
                     /translation="MAADPAETGFDLPAGAFRALDWAATGDTGPTALLLHGLSGVADV
                     WCDTVGALGESRPRCVAVDQRGHGHSPATPGRYRVGDHVGDVRTLVGRLGAPVHLVGH
                     SMGARVAFVTAARRPELVASVVVVDIGPEAWVANIDRTTRMFRSMPTRFADRAEALEF
                     GQLYGSGPGAAERFVEQRLRPQPDGTFTWLASADGLVETVSAHRRRNFWAEWDALAVP
                     TLLVRGGTSGELRPRTFEQMRRRNPRVEVVELDGVGHNVPLLAPQRLAAEIVTFWSRL
                     PVDGERDADQRQ"
     gene            complement(13756..13896)
                     /locus_tag="PLZ72_08325"
     CDS             complement(13756..13896)
                     /locus_tag="PLZ72_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36339.1"
                     /translation="MSKLRMTAAAIALAFGATVVLPSCAGVYVDSSGKTCVIVLAIPI
                     CN"
     gene            complement(13950..15182)
                     /locus_tag="PLZ72_08330"
     CDS             complement(13950..15182)
                     /locus_tag="PLZ72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNA36340.1"
                     /translation="MAAIVVLGGAGGVGRVAVEALTHIDSVSEVVVADRRRAEAAAVV
                     AELDDPRLTAAAVDVSSAASLSSLISGAAVVLNCVGPFYRFGPPTLRAAIDAGVGYVD
                     ICDDLDATRAMLEMDGDARAAGVTALIGMGNSPGLANIFVKLCADGLLDEVTTADICH
                     VHGGEPFEGAAVLKHRIHAMVDDVPLFIDGEQITVRQLDESGAAHVRDVDFAGVGTYP
                     TFPYPHPETVTLPMTFPTLQRATNLGVVYPLSYFQLTQDLVRAGMASEEPVAVGDALV
                     APIDVMVALLQQQRPRLLAEAGVEEAGGCLHVEVGGIKDGESHSYVFSLTSRGAGAGE
                     GTGIPAALGAALHVRGSFDGHAGVHAPEAIVPVDELLTLAADVVTAMSIGSGDGIPLT
                     VEHVGPDGSAQILPWSFS"
     gene            15295..>15491
                     /locus_tag="PLZ72_08335"
     CDS             15295..>15491
                     /locus_tag="PLZ72_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNA36341.1"
                     /translation="MDEFDYIVVGGGSAGCVVAARLSEDPSVSVCLLEAGGEGRDVLI
                     RAPLGFAAAMPRGINSWNYETV"
BASE COUNT         2365 a         5335 c         5579 g         2212 t
ORIGIN      
        1 gggggatcgg agatcccgtg gtcgatcaag aacaacgcga tccacgggaa cctcaccgtg
       61 gtcggccaga cctccgagtg gatcggcatc ctgttcaacc ggatcgaccg caacgtgatc
      121 ctggccggga tcaccctgac agggaccgac gactcgaccg ggaccggcac caactcgctg
      181 ttcgtggtgc gcaacacgat cggtcgcaac atcgcctgcg gatggatcga ccccggcgtg
      241 ttcggctacg gcaactcgat ctcgggccac gcgctcggtc tgtgcgaacc gctgacggcg
      301 agctgacaga gcagtctcac ccggggatcg gtgcccagga tcacggcagc cgatctcgcg
      361 acgtggcagg ttggtgggcg gagggggagc gtgtccacgc tcccagccca cgatggagct
      421 cgacaacccc accccgatcg actcgcaggt cgtccgctgc ggaccgctga ccgatgccga
      481 ccgcgacctg atcgcggcgg ccaccgaggt ggtcgaccgc tgctccgacg gagccaacca
      541 cacggtcgct gcggcagttc gggacgcgtc gggacgcatc cacaccggcg tgaacgtgtt
      601 ccacttcacc ggcggaccat gtgcggagct ggtggtgctc ggtgtggccc gcgccgccgg
      661 agccggtgag ctggaatcga tcgtcgccgt cggggacgag gaccgggggg tgctctcgcc
      721 gtgcggacgc tgccgccagg tgctcgccga cctccacggc ggtctgcgcg tgatcctgcc
      781 caccggcggg gcgctcgcag ccgtctccgc agtcgacctg ctcccgttcc gctacgagca
      841 gacctacgag cggcgcctgc gctgaggtcc ggcaacggac gctgtcgcgg agtcaggctc
      901 tccgcagggg ctggtggccg acgacgtcgg tgatggaccg gaacccatcg acgagcttct
      961 cgaggagcac gtcgtcgtcg ggaacggctc cgaggtccac cgacacgccg atgcaggccg
     1021 tctcgccgac agacaccagg gcgacgttga ccgcggcgcc gctcgtcggc gccatggcgt
     1081 actcgcggac gatctccgat ccagcgaggt agcagcccga gggaaggccc ggcacgttgg
     1141 tgatgacggt gtcgatgccc ttgagcatgg agcccatgac agcgctcacc actggaggcg
     1201 ggaggacgtc gagcgctccg gccacggcgt cggtgagggc gagcgccctc gagtgctgcc
     1261 agctgtgggc gatcgctccg atggtccgca tctgttcgat cggcggcatc gcccttgccg
     1321 gcactgcgaa gcgcgcgggg gtgaaccggt tgccgccgac ggcgtccccg gtgcggcgca
     1381 tgctgaccgg catcgtgatc gacaggtgct ccagctccgt cccgtggtcc tggtggtagc
     1441 ggcggagacc ctcgaccact gcggccatga acgcatcgtt cagcgttcct cccgccgcgt
     1501 gtccagctgc tctgaggtcc tcgaggctga cgtcgatcga gtggaacctg cggcggaggc
     1561 tccggcgctc catcagcgga ctcgtgacac gcaccggcga gagcaggcgc agcacgccgc
     1621 cggccagctc gccgacatcg gtcacggtgc ccaacgggtc cttcagcgtt gccgtcgccg
     1681 ccgtgagcgc ggccgacgac acggcgagcg gaacgcgcgc agctcgtcgc agtccctgtg
     1741 cgccgggagg agtcgcgaga tgcgggctca gccgcagcga caggtcgtcg ggctccggct
     1801 ggtccctcac gtcgtcgagc aacaggaggg ccaactccat cccaccgacc ccgtccgtga
     1861 ggcagtggtg cagcttctgc acgagggcgg cacccccgcc cctcatccct tcgaccaggg
     1921 tgaactccca caggggtcgc accgggtcga aggagtcgga ggcgatcggc tgaaccagat
     1981 ccaggacgtc ccgcagtcga ccggggaggg ggacgcggac gcggcggagg tggtggtcga
     2041 ggtcgaagtc cccgtcgtcc acccagcggg gcgctccgat gccgaacggt gccggaccga
     2101 cccgctgtcg catccgtggg atgagctggc tggcccggtc gatgcgcgtg cgaagacgac
     2161 cccagtccgg cgcccggtcc agcaaggcga ccgcggtgat cgtggaacgc agcacgggat
     2221 cgcggtccag gtcccacaac acggtgtccg acgcgctcag gcgtcggctc cgggtggggc
     2281 ccgcaggcag ttcctggtcg gcgtccggtg cagcatcgtg gtcggtcatg gccgacaacc
     2341 cttcgtcggg ggttcgcctc gtggtcatca tgtcctcccc ggacggcgcg gaggaaggga
     2401 cgaaggtcac ccgaacgcga ggagagccga acgggtcgag tcctcgggag cgaggtcggt
     2461 ccggacctgt tcggcttcgg gccagggacc gaatcgacgg gccatctgtt ccgccacctc
     2521 gggggtggcc tccgagaggt cgacgccggc ggcgagacga tgtcggatgc gctcgacggt
     2581 catgccggcg ggagcgacgc actcgatctc gaccacgtcc gccgcaaccc gatcggccac
     2641 cgtccgggcg agccgacggt gctcgggtgc cgaccaggtg gcgtcgagca ccacgtgccg
     2701 accgtgagcg aggtggtgct cggcctcggc tagcaggtcg gcgtagacct cgtccgtggt
     2761 ggtcggggcg taccgacctg cctctgggtg ccgtcgtcgc agctcgtcgg agcggaggac
     2821 gaccgcgtcg acccgctcgg caagtcgtcg tgacagcgtc gtcttgcccg tcccgggaag
     2881 accgccgacg agcaccgctc gaacgcgggc cgcctcgagg tgccggcggc acagggcctg
     2941 caaccggtcg acctcggcgg tgctgccggc tccctgaccg gcgcggatga caccgacctt
     3001 ggctcgcacg tgggcccggt aggcgacgta gtggtgctcg agagccggcg gccacgggat
     3061 ccccgagcgc tgaccgaatg cgtcgaggaa gaggcgggcg agctccggtc gcccgaagcg
     3121 ctccaggtcc atggcgagga acgcagcgtc gcccagcacg tcgccccagc ggagggtgtc
     3181 gtcgaactcg aggcagtcga gaacccgggg gccgtcgtcg agcaggaaga tgtcctcggc
     3241 ctgcaggtcc ccgtgtccgt cgcggaccgc cccgtcccgg atgcgctgct cgaacagcgg
     3301 ggcccgtccc gccaggaacc tcagcaccag ctcctcgatc cgctgctggt cggtcgcccc
     3361 gaggagggtc ccgacgtggg ggcgcatcga gtcgaagccg tcgcaccaca ggcgccggat
     3421 ggcgtcgcga gtcgccaccg actccatcgg cccgggcggg ggcgatgcat cgtgaaggct
     3481 ggccagctcg tcggcgatcc gttcgagagc gacatgcacg tcctcgcctc gttccaggca
     3541 cgctcgcagc cggcggtgcg gcggcatgcg cctcatcacg accaggtgct cgcacacctg
     3601 accatcggga tcggtcatct gggcgacccc caggtacacg tcgggggcga ggcgtcggtt
     3661 gagctcgacc tcgcgtcgac aggccgcggc tcggccctcc tcggtgcgga agtcgaggaa
     3721 ccccagatcg acgggcttct tgaccttgta gacccggtcg ccgaagaaga acagcgacgc
     3781 ggagtgggtc tcgatcgccg ccgcggagcc gacgtgtccg atcacccggg gacgaccacc
     3841 aggcgcccgt cgacctctcc acgggccaga cgtcggtagc cgtcgggcgc ttcgtcgatc
     3901 gagatccgtt cgacctcggc gcggatcagg ccccgttctg ccagattcag gacctccatg
     3961 agctccggga tgctccccca gtaggtcgtc gccagggacg cctcgtaggg gacggtgaag
     4021 aagctgaacg ggtaggaccc gccggcgatc ccgaccacgg tgatgtggcc gagggagcgt
     4081 ccagcggcga cggcgagctg gatcgtggag tcgctgccga cgaagtcgag gacgacgtcg
     4141 gcgttcttcc ctccggtcag atcggcgatc tccgcggctg cgtcgtcgcc ggcctggacc
     4201 gtgtgctccg caccggccgt cgccgccaga tcgagcgccg acgtgcgggt gtcgaccgcg
     4261 atcaccgtcg ccggggtcac agcctcgagg atctggacgc ccaggtggcc caggccaccg
     4321 actccgatga ccagcgcggt ggaacccgcc acgagcaggt ccgctgaccg cttgacggcg
     4381 tggtagggcg tgaggcccgc gtcggtgagc ggagctgctt ccaccgggtc caggtcgtcg
     4441 agcggcacca ggtgtcgggc atcgtgaacg agcatgaact gcgccatgcc gccgtcgtcg
     4501 ccgagtccgg ccgccatgga ggtgagctcg gcctggtgct cgcagtagtt ctgcatcccc
     4561 tgcacgcagc gatggcatcg cccgcagccg gtcgggccgt agaccgccac gggctggccg
     4621 agctcgagcc cggagactcc gaccccgacg gcctcgaccc agccggcgtt ctcgtggccc
     4681 agggtgaacg gcggttcgaa cggaaccatc cccgactcga agtcgtggat caggtgcagg
     4741 tccgagtggc acatccccgc accggcgatc cggacgagca cctgtccggg acctgggtcg
     4801 ggttggggga cgtccctgac ctcggggtcg tgcttccact gggtgagctg gactgcgtcc
     4861 atggcgttct tccccctgtg tcgtggtgtc aggaccagtc gagcacttcc ggcgccggtc
     4921 gaacagggtc ggatgtcagg tcgtccggct gtgggaatcc cgcggcggtg tcgagcgggt
     4981 caaccggttc ggtcccgacc acgccgggcc gagtcgacga ggaggcggcg ctcggcggcg
     5041 ccgaccgctc ggcgagccgc gtcgacggcg tcggggttca ccgagatcga gtcgatgccg
     5101 agccggacga ggtgtgcggc gaactcgggg tcgttcgacg gtgcctgacc gcagagcgat
     5161 gccgtgatcc cggagtcgtg cgcggcccgg atgatgcggg cgatcgcgtc gaggaccgcg
     5221 gcgtcggact cgtcgaacaa ctcggcgcag accgtggagt cgcggtcgac tccgagcagc
     5281 agttgggtca ggtcgttgga tccgatcgag acaccgtcga tgcccatcgc ggcgtactcg
     5341 gggatgcgat ggaccaccga cgggacctcg gccatgaccc atcggtggag gttccggtgc
     5401 gtcgccaggg gactggcgtc gacgagctcg aggcagcgct ccagctccca gcgggtccgc
     5461 acgaacggga tcatcagatg gagattcggg gtctcgtcgc ggacccgggc gagcagctcg
     5521 agctcgaggg cgaagaggtc cggctccttg acgtatcgga agcagccccg gtagccgatc
     5581 atcgggttct cctcgaccgg ttcgaactgc tcgccgcctc gcagcccgcg gaactcgttg
     5641 ctgcggaagt cgatcgtgcg gtacacgacc ggccgcgggg tgaaggcgcg ggtgatgcga
     5701 agcagcgagt ccgacatccg ccggaggaac tcgtcacggc cgccctgggc cagcagctgg
     5761 cgtgggtgca cgccgtcgag ggcgtcggcc accatgaact cggcccgcag caggcccacc
     5821 ccgtccaccg gcccggcagc gacctcgacg gcatgctcgg acatggcgag gttgacgtag
     5881 atcctggtgg cgagcgcagg gacctcgaga ggggcggcgt cggccgcgcg gggttcgacg
     5941 gcggtggccg gggcgacgac gggcaccgct cctctgagca ccgtgccccg acggccgtcg
     6001 acggtgacga cctccccgtc gcggagcagg gtggtggcgg tgcgagctcc gaccacgcac
     6061 gggacgccga gctcgcgcga cacgatcgcg gcgtggcacg tcatgcctcc gctgtcggtg
     6121 accaccgcgg cggctctgcg gatcgcaggg acccagtcag ggttggtgag cggggccacc
     6181 aggacttccc cggagagcag atcggcggcc cggtcagggg tcatcaggac ccggacgcgc
     6241 ccggacgcag tgccggatga ggcggccaga ccctgcagga gcgttgcggc atcatcggct
     6301 gtgcccgccg tgggctcgcc gctcggaccg gtgtccaggg tcgtgatcgg tctcgactgc
     6361 accaggtagg tgcggccctc gccgatcgcc cactccatgt cctgcggctc gccgtagtgc
     6421 tcctcgacgt gcaggcccag ttcggccagc tcgatcgcct ggccgtcggt gaggacgcgg
     6481 tcatcagcgt cagcggatcc cagctcctcg cggtcgactc ggccgccgga gctggcgacg
     6541 aggcgatggg tctgatgccc gacgcgcacc tcgcggatgc gagggccatc cttggtgagg
     6601 gtgtaggtgt cgggctgcac ctgtccggac acgacgacct cgcccagacc cagggccgcc
     6661 tcgatgacga tcaggtcctg atcaccggtc gatggatcga cgctgaacat gacgccggcg
     6721 cgctcggatg gcacaagctg ctgcacgacc acggcgatcg agggttcgtc ggtgagggac
     6781 tgcccggcgc ggtaggagat gacccgctct ccgtacagcg acacccagca atcgagcagc
     6841 cgccggatca ggtcgtccgg gccggcgacg tcggtgaagg tcgagtgcat cccggcgaac
     6901 gaggtcccag cggcgtcctc ggcggtggcc gaggagcgca ccgccacggc cacgtcgtcg
     6961 ccgagtcggt ggtaggcgtc gaccagggcc gtccggagag caccgggaac tccggcccgg
     7021 cggacggtct cctgcaggtg ccgggcccgt gcgcggagct cgacgggatc gtcggccgat
     7081 gcgacggcct cacggaactc cgacaggagc tcccggcgga cgcctgcagc atccatggcg
     7141 gccaggtagc cgtccgcaca caccacgaac ccgggaggaa ccgggaagcc ggcggaggcc
     7201 atctcgccga ggttcgcacc cttgccgccg acgcgatcga cctctgcgag cgagacctcg
     7261 tcgagccagg cgatcgctcc gtcggtggtg gtcgcgctca tgacacctcc aggtctccgg
     7321 actccagctt gcaccagggg acagcgcagt gttgagggac caaggtcctg cctcgtcggc
     7381 ggatcaggtg ggcgacgtgg ctcacggtgg acccacgcgc aggaccgccg ctccggtcaa
     7441 cctgtcgtgg gcgagatcgg ccaacgcctc atcggcgcgc tccagcgggt acgcggtcgt
     7501 gtgcacctgc acgccgatgc gtccggcgat gcgcagcagg tcctctccgt cacgacgggt
     7561 gttggcggtc acgctgcgga tctggcgctc ctggaacagc tcgcggtcgt agtcgagcgg
     7621 aggggtgtcg gacatgtgga tgccggcgac ggccagggtt ccgccgcgat cgagcgcgcg
     7681 gagcgcggcg gggacgacgt cgccggatgg cgtgaagttg atcgcggagt cgaggggttc
     7741 cgggggtgcg tcggtggcgc cgcccaccga cgcacacccg agcgccagag cgagctcccg
     7801 ggcttccggc gaccgggtca tcacgtggac cgtcgccccc tgcgcgatcg cgatctgggc
     7861 ggtgatgtga gccgatgctc cgaacccgta gatgcccagg cgtccaccgg ggggaagttc
     7921 ggccctccgc agtgctcgga acccgatgat cccggcgcac agcaggggtg ccacgtgctc
     7981 gtcggcgaac cccgcgggga gtgagtaggc gaagcgctcg tcgaccacgg cgtgctcggc
     8041 gtagccgccg tcctcgtccc agccggtgaa cgacggggac gtgcacaggt tctcggctcc
     8101 gcgccgacag aaccggcaca cgccgcaggt gcgcctcagc caggcgatgc cgatccggtc
     8161 gccttcggcg aagcgggagg ctcccggccc caggcggtcg accacgccga cgatctcgtg
     8221 gcccgggacc actccggccc ggtgcactgc gagatcgcct tcggctacgt ggaggtccgt
     8281 ccggcacaca ccgcagaccg agacccgcac ccggacctcg ccgggaccgg gttcggggtc
     8341 gggacgatcc gacagctcca acgggcccct gtcgatcggc gccggagcgg tcacggacca
     8401 cgctctcatc cgccccaccc tgccacagcg accccggccg accgcggcgc tctcggcgtc
     8461 agccctggta ggcgggggtt ccgtcgaggt aggtctccga cacggtgatc tcgggccagc
     8521 cgtcgggatc gctggcccac ggatcgtcgg acacgaccgc gaagtcggcc cgctgtccga
     8581 caccgagact cccgcggtca tcggcgtgga tctgccacgc cgcgtgcgtc gtgatcgccg
     8641 ccagggcctg gtcgggcgac acgcactcgg ccgggttgag caccgtgccg tcggcttgca
     8701 gccgtcgctg caccgcggtt cgggcgctga gcagcgggtg gatctcggtg acggtgtagt
     8761 cggagtgcag cgacgggcgc atgcccgccg cgtacgccga cgcgacgcgg tcgagtcgct
     8821 gggcacgctc cgggccgagg atcgtgtcgc ggaacgcggc gccccagaag tagacgtggt
     8881 tcatcaggaa gctgggcgag atcccggccg cggccatccg ggtgagctgg tcgtcggtgg
     8941 agaacgagac gtgctcgaat cggtgccgca ggtccccggc cggatggtgg gggagcaggt
     9001 cttccatggc gcacagagcg aactcgaccg ccgcgtcgcc gttggtgtgc accatcacct
     9061 gccagccgtc gctcagcccg acctccagca cgtcgcggag tccctcggga gtccagttgg
     9121 ccttgccgcc ggactcctcg cccaggtacg gctgcaggaa gtagccgctt cgtccctggt
     9181 tcgagccgtc ggtgacgacc ttccaggcat cggcgcggac catgtcgtcg ccgctgaacg
     9241 gggtgacgtc ggcatcgcgc catgcggcgg cgacctcgga gggagtgcgg tcccccatga
     9301 tggagaactg ggcggtcgag acccgcaccg gcagccgtcg ggatccgttg atctggtgga
     9361 gcagcgcgag ctcgccgacg ccgccgaggg tgccggtggc cgcctcacgc agcgatgtca
     9421 cccctcgacg ggccgcagcg tccaggatgc gacggacgcc gtcggtgatc tggtccgagg
     9481 tcggcatcgg caccgcagcc atcagggcca tcatcgccgg cacctcgccg atcaccccgt
     9541 tcagacggcc gtcgacccgg ccgaacgtcc cgcccggcgg gtccggtgtc gtgtcggtca
     9601 ctccggcggc ggccaacgct gtcgagttgg cgtacatgta gtgcagcgat gcgttgacga
     9661 cgaccacgga tcgatcgggc gagatccggt cgagcagatc ccgggtcagg acgggttcgc
     9721 cggggtagag gctgggatcg aactgcttgg ccatcagcgg ctccccgggc gctgctccac
     9781 cgacggcgct ccgcagcgcg ctcacgacgt cgtcgaaggt cgggcacgcc tcgtgggaga
     9841 ggtcgagcca gtcgaggttg agcaacgagg cccagatgtg catgtgcggc tcgaccaggc
     9901 ccgggatgac cacgccgtcg ggggcgacga tcgtggtgga gggaccggtg aggccgtcga
     9961 cgtcggaccg ggatccgatt ccgacgatcc gcccgtcgct caccgccatc gcctcggccg
    10021 agggcgtcga ggggtcgccg gtggagatgt ggccgaccac cagcagatcg gcctgcgggg
    10081 tcgaacgggt cgatgtccgg cgcccgtggt tcgcgggaga gatcgtcgta ttggtcgatc
    10141 ggagcaccgg accgaggcgt tcggcggcga tcgggtggac ggtgcagcac gaaccggtgg
    10201 tcacgagggc tcctcctggc gtcggtgtcg tgacggtacc ggtgcgttgg tcacgagcgc
    10261 ggcgacagct ggcgtgctgc tccgaggaag agcaccgagt tcgccgcgac cagcgtggtg
    10321 acgggttcct tctggccgtc gaggtaccac cggatcgcgg tcatgatgac gctgcccacc
    10381 agtccggtgg cgatcaggcg cagccgctcg tcgggggaga ggccgaacgt ctcggccatc
    10441 accgtggcga ccaggtgggc gaaccggtcg atccccgaga tgtaggtgga gtcgaccagc
    10501 gtgctcaccc cgagcacctc cagccagcac acccgcagca cccgcggatc tgccaccgag
    10561 gcgtagaacg cctcgagggt cgtgcgcacg acgtggtcga gaggttcggc tgatgagccc
    10621 ccagccggcc ggtcaccggt ggccatggcg acgaggatgt cgtcgtgcat ccggtcggtg
    10681 cagtgctggt agacgccgat caggagggct tcggtgttgg cgaaggactc gtagaagtag
    10741 cgctcggaca ggcccgcctc acggcagacc gcccgcacgc tcgtcgactg gtagccacgg
    10801 gttccgaaca gctcgagtcc ggcggccagg aagcggtcgc gacgttgggc gacgcgcacc
    10861 tcggcgggga ctccgccgta ggagcgaccc gacgcccggg atccggagcg tcgtgccgag
    10921 gagcgatcga gaggtgcggc catcgcagtg attgtggccc agagcgacgc cgtcgcccgc
    10981 cggcgtgaca cacgttcgct tgacccggca cgctcgccgt cggaaagtga caacactcat
    11041 tgccagatga ggcggacccg gaccgccggt gcgaggaggt agcggtggac gtccacgagg
    11101 tgatcgtcat cggcagcggc ttcagcggcc agagcgcggt gatcggcctg cagaaggccg
    11161 ggatcgagga ctggatcgtc ctcgaacgcc gcagcttcat gggtgggacg tggtgccaga
    11221 acacgtaccc gggcgctgcg gtcgacgtgc agtcgccgct gtactcgatc tcctccgagc
    11281 cgtacccgtg gagccagatc ttcgccgagc gcgacgagct ggagcgctac accaacttca
    11341 tcatcgacaa gcaccacatc cgggagcgca ccgagctgaa cgccgatgtg tcggccctcg
    11401 actgggacga cgactagcgt cactgggtga tcagcacccg cgacggccgc acgttctgtg
    11461 cccgcttcgt gatcctcggc cccggtccgc tcagcaaccc gaagatcccc gacttcgagg
    11521 ggcgggacac cttcgagggg cactcgttcc acagcaacgc ctgggaccac gactacgacc
    11581 tggccggcaa gcgcgtggcg gtgatcggca gtggcgcgag cgcagcccag atcatcccgg
    11641 ccatcgcccc cgaggtcggg gagctgcacg tcttccagcg caccccgcac tgggtcctcc
    11701 cgcggcggga ccggaagttc tctcggctgg agcgcgcgct gctcaagaac cgtcgcgtct
    11761 acggcgcgct gcgctccgcg atctacctcg gcctcgagac gagggtgatc ggcttcaagt
    11821 acttcccgcg gctgctcgac ctggtggcca agcgccaggc gctcgcccac atcgcccgac
    11881 agatccccga tcccgagatc cgccgcaagg tgacccccga ctacacgatc ggctgcaagc
    11941 gggtcatcgt ctcgtcgacc ctgtaccccg cgctctgccg accgaacgtg aagctgcacg
    12001 actcgagcga cggcatcgac gagatcgtgc cggccggcat catcaccacc ggcggcgaca
    12061 aggtcgagct cgacgccatc gtgtacgcca ccggctacga cgccaccgac ggcgcgacga
    12121 ccttcaccgt caccggccac gactcgactc cgatcaccga cctgtgggcc gagttccccc
    12181 gcgcctacct cggtacgacc atgccggggt tcccgaacct gttcctgatg ctcggaccca
    12241 acaccggcat cggccacacc tcggcgctgt tcatcatgga gtcgcagttg cgctacgcgc
    12301 tcgactgcat ccagcgcacc cggcgcaaca accgccacgc catcgagccg tcggtcgagg
    12361 ccgaggaccg ctacacggaa tgggtgcacc gggccatgga gcggaccgtc tggaagtccg
    12421 gcggatgcag cagctggtac cagaactcga gctcggggaa ggtcatcgcc atgtatccgg
    12481 gcttctcgtt cagctaccgg ttccgggccg gtcgcttccg ctccgaggac cacatcttca
    12541 cctgacgggc ctgatcacca gcgacggcgg tcggcgatgc cggcgtggac ccggtcgtcg
    12601 ctggctgcac ctacgatcgg acgtatccga cgtccagaga ggtgcgtgag atcccatggc
    12661 tgacacgtgg ttgcagaccc tgatcaccga gaccccccag gagggcttcg agctggcgat
    12721 caccctgagt cggagagggg tgaagtacac ccagcccgac accgacgtgc tccacgagct
    12781 acggcccgac tacgcgaact cggcggactc cctgactgcg gcgtcgcagg tggtggcggt
    12841 caacttccag acggtggccg cggccaacaa ctactggcgc tgatcggcgt cgcgctcccc
    12901 gtcgacgggg agtcgactcc agaacgtcac gatctccgcg gccaggcgtt gcggagcgag
    12961 gagcgggacg ttgtggccca cgccgtcgag ctccaccacc tcgacgcgtg ggttgcggcg
    13021 gcgcatctgc tcgaaggtgc ggggtcgcag ctccccgctg gtcccaccgc gcaccaggag
    13081 cgtcgggacc gccagcgcgt cccactccgc ccagaagttc cgtcgtcggt gggcgctcac
    13141 cgtctcgacc agaccgtcgg ccgacgccag ccatgtgaag gtgccgtccg gctgtggccg
    13201 taggcgctgc tcgacgaagc gctcggccgc cccgggtcca gatccgtaga gctggccgaa
    13261 ctcgagcgcc tcggcgcggt cggcgaaccg cgtcggcatt gagcggaaca tgcgggtggt
    13321 ccgatcgatg ttggccaccc acgcctcggg cccgatgtcg acgaccacca ccgacgccac
    13381 cagctcggga cggcgagcgg cggtcacgaa cgcgacacga gcccccatcg agtggccgac
    13441 caggtggacc ggcgctccga gccggcccac gagcgtccgg acgtcgccga cgtggtcgcc
    13501 gacccggtag cggcccgggg tggccggact gtggccgtgg ccgcgctggt cgaccgcgac
    13561 gcatcgcggc cggctctctc cgagcgcgcc gaccgtgtcg caccagacgt ccgcgacccc
    13621 gctgagaccg tgcagcagca gggcggtggg accggtgtcg ccggtggcgg cccagtcgag
    13681 ggcgcggaac gccccggccg ggaggtcgaa cccggtctcg gccgggtcag cggccacggc
    13741 gggcggcgag ctcgatcagt tgcagatcgg gatggccagg acgatcacgc acgtcttgcc
    13801 cgaggagtcg acgtagaccc cggcgcaact ggggagcacc accgtggcac cgaaggcgag
    13861 ggcgatcgcg gctgctgtca tgcggagctt ggacacggag cggatcctaa ccacggcggg
    13921 gtgggcgacg acgggattcc cgccgtcggt cacgagaagc tccacgggag gatctgtgcc
    13981 gagccgtcgg gcccgacgtg ctcgacggtc agcgggatcc catcgcccga tccgatgctc
    14041 atcgcggtca ccacgtccgc ggccagcgtg agcagctcgt cgaccggcac gatcgcctcg
    14101 ggggcatgga cgccggcatg gccgtcgaac gacccgcgca cgtgcaacgc ggcgcccagc
    14161 gcggcgggga tgccggtgcc ctcgcccgca ccggcaccgc gcgatgtgag ggagaagacg
    14221 taggagtggg actcaccgtc cttgatcccg ccgacctcga cgtgcaggca gccaccggcc
    14281 tcctcgacac cggcctcggc caacaggcgc ggacgctgtt gctggaggag agcgaccatc
    14341 acgtcgatcg gagcgacgag ggcgtcgccg acggcgaccg gttcttccga tgccatgccg
    14401 gcgcgcacca ggtcctgggt caactggaag tacgacagcg ggtagacgac gccgaggttg
    14461 gtggcccgct gcagcgtggg gaacgtcatc ggcagcgtga cggtctcggg atgcgggtag
    14521 gggaaggtcg gataggtgcc gacgcccgcg aagtcgacgt cgcggacgtg ggcggcaccg
    14581 ctctcgtcca gctgtcgcac cgtgatctgc tcgccgtcga tgaacagcgg aacgtcgtcg
    14641 accatggcgt ggatccggtg cttcagcacc gccgcaccct cgaagggctc tccaccgtgg
    14701 acgtggcaga tgtcggcggt ggtcacctcg tcgaggaggc cgtctgcgca caacttgacg
    14761 aagatgttgg ccaggcccgg cgagttgccc atcccgatca gggcggtgac ccctgcggcg
    14821 cgggcgtcac cgtccatctc caacatcgcc cgggtggcgt cgaggtcgtc gcagatgtcg
    14881 acgtaaccca cgccggcatc gatcgcggca cgcagggtcg gggggccgaa gcggtagaag
    14941 ggtccgacgc agttgaggac gacggcggct ccgctgatca acgacgacag ggaggcggcg
    15001 ctgctgacat cgaccgccgc ggcggtcaac ctggggtcgt ccaactcggc caccaccgcc
    15061 gccgcctcgg cgcgtcggcg gtcggcgacc acgacctcgg acacggagtc gatgtgggtg
    15121 agcgcctcga ccgccacgcg acccacgcct cccgcaccgc cgagcaccac gatcgctgcc
    15181 atcccgcctc ctcggtcggc cgcccggtcc tcatcgtgcg gagccttccc cacgctaggc
    15241 cgacccggga cccggcccgg tcttcgtcgc ggcagggacg gtacggtgcg ggtcgtggac
    15301 gagttcgact acatcgtcgt cggcggcggc tccgcgggct gcgtggtggc cgctcgcctg
    15361 agcgaggacc cgtcggtcag cgtctgcctg ctcgaggccg gcggcgaggg ccgcgacgtg
    15421 ttgatccgcg ccccgctcgg cttcgcggcc gccatgccgc ggggcatcaa cagctggaac
    15481 tacgagacgg t
//
LOCUS       DAOQOD010000068        15468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1360_length_15468_cov_12.261468, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000068 DAOQOD010000000
VERSION     DAOQOD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15468
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_15468_cov_12.261468"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1645
                     /gene="ahcY"
                     /locus_tag="PLZ72_08340"
     CDS             194..1645
                     /gene="ahcY"
                     /locus_tag="PLZ72_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNA36342.1"
                     /translation="MTFTDFKVADLALAPIGRKQIELAEQEMPGLMAIRAEYTDSRPL
                     AGARITGSLHMTVQTAVLIETLTALGAEVRWCSCNIFSTQDEAAAAVAVGPDGTVEDP
                     RGVPVFAWKGETLDEYWWCTEQVLTWPADTEGHTGPNMILDDGGDATLLVHKGTEYEA
                     AGAVPDTVEEDSDEWGVVLSTLRRTLAEDSQRWTRIGAGIKGVTEETTTGVHRLYQMF
                     EQGLLLFPAINVNDSTTKSKFDNLYGCRHSLVDGINRATDVMIGGKLAVVCGYGDVGK
                     GCVQSLRGQGARVVVTEIDPICALQAAMEGIQVVRLEDVVETADIFITTTGNKDIITA
                     EHMARMKHNAIVGNIGHFDNEIDMAGLYRQSDIRKVNIKPQVDEFVFPDGHSVIVLAE
                     GRLLNLGCATGHPSFVMSCSFSNQVLAQIELFTKTADYPLGVHVLPKQLDEDVARFHL
                     DALGVRLTTLTPEQSAYLGVPVEGPYKPDHYRY"
     gene            complement(1695..3707)
                     /locus_tag="PLZ72_08345"
     CDS             complement(1695..3707)
                     /locus_tag="PLZ72_08345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNA36343.1"
                     /translation="MTDTPDVADEHPAPPPSIGTLPGLTGLRGIAVVAVVAYHLGYLP
                     GGFVGVDLFFVLSGFLITSLLMSRPPVGAAGLRRWWARRVRRLTPAVAVVVLAVLALF
                     LTRSGIALDAVATMTWWQNWHLIAEGTSYWASSPSPLRHAWSLSIEEQFYLLWPVTLL
                     GLLALGRRWRRPAAVVAGAAALGAAASFLWASWLSRADGADLSRIYFGTDTRIGALLV
                     GCAAAAWMQRDPQRWQRPPRVRPAVSVTAVGAGILLAVLSVSLAPDSLWSYRGGLALV
                     ALGALVLVVACSGPGPVSSALSGRVLQWLGTRSYAIYLWSWPVQLTVQERWPDLELRW
                     VAATTALTSLLLADVSMRVVENPLRFGTGWAQGIRPRQAAWGLGLVALVVGSAVAARS
                     TVPTTAERLAQEFEQVPDPTTTVTAPGDGTGPTDGVAPTAPPTTVCVPTTDVATQFSG
                     GDRKFDPSTVEGIGDPVGDKCGATRVLVVGDSTGRGAANGLRRAPASGLEVWDRTVLG
                     CGIEVNPAKCPSWRTIWPAAVQEVRPQVVLVHLGVSVDVVDGPEPRFTAPEAAGVRQQ
                     VIGEAMDALAATGARVVWTLPAVPLDNGTFYCGGSRKGTGCDPDWIARWNADVVAAAT
                     PRGVAVVNLEDWVASRGRTKADRPDGLHFSGAGLDAEAALLAPQLR"
     gene            3924..4748
                     /locus_tag="PLZ72_08350"
     CDS             3924..4748
                     /locus_tag="PLZ72_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA36344.1"
                     /translation="MTQDHPIDLRHPRTHRPGRHDVGTSPTPPRPLSMVYGGGGMFGI
                     GYCVGVAHGLAAAGVPVDSAPALGTSAGSWAAAVVALGVTYDEIAGIDAPRVPNRRPG
                     VLADAAESVFGAATHPLVSVSAVCVRSRRRHILDGDRYRLSDLVAASSAVPGLLPAHR
                     VDGRLYVDGGVRSATSVDAAPAAESVIVVAPLAGPHLGAMGRGAGLLLQREVRAFRRR
                     YPDRRITVIRPDRAIAAHAGRNPMNLFDADRARAVYPLAVDQGVRCAERLAAAPVV"
     gene            4809..5675
                     /locus_tag="PLZ72_08355"
     CDS             4809..5675
                     /locus_tag="PLZ72_08355"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36345.1"
                     /translation="MDGFEGKVAVVTGGASGIGAAMAAAFAARGMKLVLADIEPGPLE
                     ETAHALRGDGADVFAVTADVSRAADVERVGQAAIDTFGALHVACNNAGVGSGGATWEV
                     DLSTWEWVLGVNLMGVVHGLRSFTPRIIASGGGHIVNTASMAGLTSPAFMSPYNVSKH
                     AVVTMSESMYVELEMLHPEVGISVLCPGWVQTRINESDRNQPDDVAAAQAAAEAADGD
                     VPVDAAQLKAMIDGFVAEGIRPAVVADMVVSAIEERRFYILTHPEWQTMVRDRVERML
                     GGENPTMALPGN"
     gene            complement(5676..6266)
                     /locus_tag="PLZ72_08360"
     CDS             complement(5676..6266)
                     /locus_tag="PLZ72_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNA36346.1"
                     /translation="MLEVLPPRRCAVCAAPGSALCGPCTSRLPPAPDREPPVGLDDCW
                     ALLAYDGETPRLVAELKFRNHRDVLSPVSRALAHFVTGSPSAVNSVTWAPTSTRRRRR
                     RGFDQAELLARGIGGRASTPVTGVLERVSAGAQTGADRDHRLLGPAFRATAPVAGHVV
                     VVDDVWTTGATLSAAAATLREAGATRVSGLVLAVRP"
     gene            6357..9071
                     /gene="secA"
                     /locus_tag="PLZ72_08365"
     CDS             6357..9071
                     /gene="secA"
                     /locus_tag="PLZ72_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNA36347.1"
                     /translation="MGFMDRLLRTGEGKKVRALADLVPDIGALEPEMQALSDEDLQHR
                     TVEFREQLDRGIPLDDLLIPAFAVVREAASRIIGQRHYDVQLMGGAALHFGWVAEMKT
                     GEGKTLVSTLPVYLNGLPGTGVHVITVNDYLARRDAEWMGQVHRFLGLTIGMILPGEQ
                     DPNFKREQYACDITYGTNNEFGFDYLRDNMALSLEEKVQRGHVYAIVDEVDSILIDEA
                     RTPLIISGRVGDAAKLYYKFASVARGLTRDVDYEVDEEKRVVFPLPDGIAKVEKSLGV
                     ENLYDDVSNSLVHQLQVSIKAKELYKRDRDYLVQNGEVKIVDEFTGRVLEGRRWSEGL
                     HQAVEAKEGVKIKEENQTLATVTLQNYFRMYDKLAGMTGTALTEAAEFMSTYNLQVVP
                     IPTHRPVQRRDDADLIYRSEAAKFSAVVDDIVERQAEGQPVLVGTVSVEKSERLSREL
                     EKRGVPHSVLNAKQHTKEAEVVTQAGRIGSVTVATNMAGRGVDILLGGNPEGLARREV
                     HAEGLEDGTEEAEARYEELLAKYEVECKAEGEEVRKLGGLYVLGTERHESRRIDNQLR
                     GRSGRQGDPGASRFYLSLEDDLMRFFATGAIEWVMGKGWDDDVPIEAKMVTRAIEKAQ
                     NTVEARNGEIRKNVLKYDEVMNEQRKVIYARRDEVLEGVDLREETLQALAEATNELIG
                     THCGSDYSDDWDIEALLGDTATYWPVELTVEDLTEAASQDGLYDLLMADAVSHYEGRE
                     AEFGADTFRQIERQIMLRIIDAKWRDHLKEMDYLQEGINLRALGQKDPLTEWQREGFD
                     MFGSLMEAVSKEYVQYVMHVQAVKEEQPRLQPQRFDYSAPDDPSSGPNPVPAVVAAES
                     AHEAAAVQADDDAAPQQPVVKSEWDKTPRNAPCPCGSGKKFKVCHGAA"
     gene            9120..10223
                     /gene="prfB"
                     /locus_tag="PLZ72_08370"
     CDS             9120..10223
                     /gene="prfB"
                     /locus_tag="PLZ72_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNA36348.1"
                     /translation="MQDFTPRISALRTRLAEAEGYLRVAELRARRPQLETELGRPDLW
                     DEPESAQALQREFAELSDDLDLFDRLESRVDDVDTLAELAREEGDESLEPEVESELGS
                     LVGEFDRLELRALFTGDYDDRDAIVEVNSGAGGVDAQDWAEMLLRMYVRWAERSGMDL
                     EIESLNEGTEAGISSATFLLRGRNAYGWMRSEHGVHRLVRISPFDNNARRQTSFASVK
                     VTPWIDDVDDEIDIDDKDLRIDVYRSSGAGGQHVNVTDSAVRITHLPTGIVTSCQNER
                     SQHQNKDRAMKILKAKLLDLERQKRAEKLAEITGEGAAVDFGSQDRNYVLQPYQQVKD
                     LRSGLELSNPEAVLDGELDDLMEAYLQWVRVGG"
     gene            10462..11139
                     /gene="ftsE"
                     /locus_tag="PLZ72_08375"
     CDS             10462..11139
                     /gene="ftsE"
                     /locus_tag="PLZ72_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNA36349.1"
                     /translation="MIKLENVTKIYKGEMVALRDASVDIDKGEFVFLVGPSGSGKSTF
                     LRLLNREERPEKGRIFVAGKDIAELPGYKVPYLRRNIGSIYQDFKLLPTKSVYENVAF
                     ALEAIGRPRSAIREAVPQILDLVGLAKKQSNMPNELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPATSVGIMKLLDRINRTGTTVVMATHDRGIVDTMRRRVIELDRGRVVRD
                     QARGVYE"
     gene            11146..12042
                     /locus_tag="PLZ72_08380"
     CDS             11146..12042
                     /locus_tag="PLZ72_08380"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNA36350.1"
                     /translation="MAVRADYILRETGSNLVRNLTLTFAAVLTVAVSLAMVAASYLIG
                     AGINNSFLGLRSDVDLFVYMNPAATAEQIKAMEKTLDSSPQVGEFDFYDSARSYDEFK
                     RLFRDQPDLVNTIKPEDLPQSFRIKPKSTDAEIVSSLGDELEAKEGVLRVEYAAEYAK
                     RMQTSVRTLNTWVLIVGGVLIAASVLLIFNTIRTAVFARRREIEVMRLVGASNWFIRL
                     PFIVEGLVHGLLGAALAAVGAWGFDQLWKRNFVDQVGFELLNQIRWDSGQLWTSILII
                     FVVGALTGGIGSGIAVSRFLKV"
     gene            12060..13025
                     /locus_tag="PLZ72_08385"
     CDS             12060..13025
                     /locus_tag="PLZ72_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA36351.1"
                     /translation="MTVGDGYRTIGYDLSDGVATITLDRPDHMNAFTVRMCLEMIDAF
                     DRVDDDPDVRAVIVTGAGRAFCAGADLVGGADAFDPSGSAAAGAASELGEGGGAPPGG
                     PAASELSPGERAMRRSVDPRRDFGGLLTLRIFEVTKPVIAAINGPAVGIGATMTLPMD
                     IRLASDSARFGFVFAARGIVPEAASSWFLPRLVGIQQALEWCYTARVFPAAEALGGGL
                     VRSLHAPEDLLGAARALATEIATNSAPVSVALTRQLMWRGLVADHPMEAHRIDSPGVT
                     TTGSGADAREGITAFFEKRPPEWRQQVPGDLPDWFPWWDQPSFDP"
     gene            13161..13763
                     /locus_tag="PLZ72_08390"
     CDS             13161..13763
                     /locus_tag="PLZ72_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNA36352.1"
                     /translation="MRPDNRHIPDIHHDLDNNHHDPFGRRVGRGRPIRGSGRGRGRGG
                     YRSRSGGRANRGDVRAAVLALLAEQSMHGYQIISELDERTGGAWKPSPGSVYPTLQML
                     ADEGLVRSEPEGGKNVFHLTDSGRRAAAEGDSAPWDAVAGDGATVDVRRTIAQLAMAF
                     KQVTHTGTVDQVERATRILNDARKAIYGILAEDPSETRSS"
     assembly_gap    13908..14007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14008..>15468)
                     /locus_tag="PLZ72_08395"
     CDS             complement(<14008..>15468)
                     /locus_tag="PLZ72_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA36353.1"
                     /translation="PLMSEELVYRRRWWILGVLCLSLMVITLDNTILNVALPTLVRDL
                     GATDSQLQWIVDSYTLVFAGLLLTAGSLGDRFGRKGALQIGMVVFAVGSALSAFATDP
                     NHLIVTRSLMGIGGAFIMPATLSILTNVFPAEERGRAIGVWAGVSGLGIALGPLLGGY
                     LVEHFSWHAVFTVNIPIAVVAIGAGVVLLPRSKDPSAPRLDPLGAVLSIAGLVTLVFG
                     LIEAPGRGWTDGVILGCFAAAAVLLTLFAVHELHSAEPMFDVTFFKNPRFTAASTAIT
                     LVFFALFGFSFLQTQYFQFVLGYSPAETGVRLLPLALTLMVVAPLSSRIVEKVGTKVV
                     VGTGLTLVTISLLLQLQLTAESPYLPVMWRMMLLAMGMGMTMAPATESIMGSLPLAKA
                     GVGSAMNDTTRQVGGALGVAIIGSVLSSVYGSKIVEALSGKVPDQALDAARSGVGGAL
                     AVAERAGDAGDGLAQLARNAFVDAMHGGAVVAAVAAA"
BASE COUNT         2485 a         5415 c         5403 g         2065 t
ORIGIN      
        1 tgtccgagga tctggtcggc gcctgacgcc gtccgggcgc ggctccggac ccaccgaggt
       61 ccctccgccc ggtcgcgggt aggctctggt cccgccaggg ctgccctgta cccggagtca
      121 gacgacccca gcccgagctc gtcccacacg acttcaccac cgcggcgcgt ccgccgcgcc
      181 caggaggcat cccatgacgt tcaccgactt caaggtcgcg gacctcgccc tggcccccat
      241 cggccgcaag cagatcgagc tcgccgagca ggagatgccc ggtctgatgg cgatccgcgc
      301 cgagtacacc gactcccgac ccctcgccgg ggcccggatc accgggtcgt tgcacatgac
      361 cgtgcagacc gccgtgctca tcgagaccct caccgccctc ggggccgagg tgcgttggtg
      421 cagctgcaac atcttctcga cccaggacga ggccgccgcc gcggtcgccg tcggcccgga
      481 cggcaccgtc gaggacccgc gcggggtacc ggtcttcgcc tggaagggcg agacgctcga
      541 cgagtactgg tggtgcaccg agcaggtgct cacctggccg gccgacacag agggccacac
      601 cggcccgaac atgatcctcg acgacggcgg cgacgccacg ctgctggtgc acaagggcac
      661 cgagtacgag gcggccggcg cggtccccga caccgtggag gaggactccg acgagtgggg
      721 cgtcgtgctc tcgacgttgc gccggaccct cgccgaggac tcacagcgct ggacccgcat
      781 cggggccggc atcaagggcg tgaccgagga gacgaccacc ggcgtgcacc gcctctacca
      841 gatgttcgag cagggcctgc tgctgttccc ggcgatcaac gtcaacgact ccaccaccaa
      901 gtccaagttc gacaacctct acggctgtcg ccacagcctc gtcgacggca tcaaccgggc
      961 gaccgacgtg atgatcggcg gcaagctcgc ggtcgtctgc ggctacggcg atgtcggcaa
     1021 gggctgcgtg cagtcgctgc gcggacaggg cgcccgggtg gtcgtcaccg agatcgaccc
     1081 gatctgcgcg ctgcaggccg ccatggaggg catccaggtg gtgcgtctcg aggacgtcgt
     1141 cgagaccgcc gacatcttca tcaccacgac cggcaacaag gacatcatca ccgccgagca
     1201 catggcccgg atgaagcaca acgcgatcgt cggcaacatc ggccacttcg acaacgagat
     1261 cgacatggcc ggcctgtacc gccagagcga catccgcaag gtcaacatca agccgcaggt
     1321 cgacgagttc gtgttccccg acggccactc ggtgatcgtg ctcgccgagg gccggctgct
     1381 gaacctcggc tgcgcgaccg ggcacccgag cttcgtgatg agctgctcgt tctcgaacca
     1441 ggtgctggct cagatcgagc tgttcaccaa gaccgccgac tacccgctcg gcgtccacgt
     1501 gctgcccaag cagctcgacg aggacgtcgc ccggttccac ctcgacgccc tcggcgtccg
     1561 cctcaccacg ctcaccccgg agcagtcggc ctacctcggg gtcccggtcg agggccccta
     1621 caagcccgac cactaccggt actgagcgcc accggcaccg aacgccaccg gtacccgacc
     1681 gcggcggggt cgcctcaacg gagctgcggg gcgagcagcg cggcctcggc gtcgaggccg
     1741 gcgcccgaga agtgcagccc gtcggggcgg tccgccttgg tccgcccccg cgaggccacc
     1801 cagtcctcga ggttcaccac ggccacgccc cgcggggtcg cggccgcgac cacgtccgcg
     1861 ttccagcggg ctatccagtc gggatcgcac cccgtgccct tgcgggagcc accgcagtag
     1921 aaggtgccgt tgtcgagcgg gaccgccggg agcgtccaca ccacgcgggc accggtggcg
     1981 gccagggcat ccatcgcctc gccgatgacc tgttggcgga cgccggccgc ctcgggggcg
     2041 gtgaagcggg gttcggggcc gtcgacgacg tcgaccgaca cgccgaggtg caccagcacc
     2101 acctgagggc ggacctcctg gaccgcggcg ggccagatcg ttcgccacga cgggcacttg
     2161 gccgggttca cctcgatccc gcacccgagc accgtccggt cccagacctc gagcccgctg
     2221 gcgggggcgc gcctcaggcc gttggcagcg ccccgcccgg tgctgtcgcc gacgaccagg
     2281 acccgggtcg ccccgcactt gtcgccgacc gggtcgccga tcccctcgac cgtgctcggg
     2341 tcgaacttcc tgtcgccccc ggagaactgg gtggccacgt cggtggtggg gacgcacacg
     2401 gtggtggggg gagcggtcgg cgcgaccccg tcggtcggtc cggtgccgtc gcccggagcc
     2461 gtgacggtcg tggtcgggtc gggaacctgt tcgaactcct gtgccaggcg ctcggcagtg
     2521 gtcggcaccg tcgatcgagc ggccacggca gagccgacga ccagcgcgac caggcccaga
     2581 ccccaggccg cctgccgggg tcggatcccc tgggcccagc cggtgccgaa gcgcagcggg
     2641 ttctcgacca cgcgcatgga gacgtcggcc aggagcagcg aggtgagcgc cgtcgtcgcg
     2701 gccacccagc ggagctcgag atcgggccag cgctcctgga cggtcagctg gaccggccag
     2761 gaccacaggt agatcgcgta cgaacgggtg ccgagccact gcaggacacg tccggacagg
     2821 gcgctgctga ccggcccggg tccgctgcag gcgaccacca gcaccagcgc gcccagggcg
     2881 acgagcgcca ggccgccgcg gtagctccac agggagtccg gagcgaggga gacgctgagg
     2941 acagcgagga ggatcccggc gccgacggcg gtgacgctca ccgctggccg tacacgcgga
     3001 gggcgttgcc atcgctgcgg atcccgctgc atccaggctg cggcggcgca tccgaccagc
     3061 agggcgccga tgcgggtgtc ggtgccgaag tagatgcgcg acaggtcggc tccgtcggca
     3121 cggctcagcc acgacgccca caggaacgac gcggccgctc cgagggccgc ggcgccggcg
     3181 accacggcgg cggggcgtcg ccaccggcgg ccgagcgcca ggaggccgag cagggtcacc
     3241 ggccacagca ggtagaactg ctcctcgatc gacagcgacc aggcgtggcg cagcggcgac
     3301 ggcgagctcg cccagtagct ggtcccctcg gcgatcaggt gccagttctg ccaccacgtc
     3361 atggtggcga cggcgtcgag cgcgatgccc gagcgggtca ggaacagggc caggaccgcc
     3421 agcacgacca cggcgacggc cggggtgagg cgtcgcacgc gacgggccca ccaccgtcga
     3481 agcccggcag cgccgaccgg ggggcgactc atcagcagcg acgtgatcag gaatccggac
     3541 aggacgaaga acaggtcgac gccgacgaag ccgccgggca ggtagcccag gtggtaggcg
     3601 accacggcca cgaccgcgat cccgcgcagg ccggtgaggc cgggcagggt gccgatcgac
     3661 ggcggtggcg ccgggtgctc gtcggccacg tcgggggtgt cggtcacggt ggtcatcgtg
     3721 cggggtgccc gcggtcgggg cggcgtcggg gacgtcgccc cgttcgatcc tgtcggatcg
     3781 ggggccgccg gggaatgggc ggatcccccc gggtcaggcg tccacgttcc tcgacgtggg
     3841 gagcattacc ttctcgtcgc ctctcgacgg tacccgtacc gtcgaggtgg cgggccccgc
     3901 ccgctcgggc gaacggaggc gacatgaccc aggatcaccc gatcgacctc cgccacccgc
     3961 ggacgcaccg gccgggacgc cacgacgtcg ggacgagccc gacgccgccg cgtccgctgt
     4021 cgatggtgta cggcggggga ggcatgttcg ggatcggcta ctgcgtcggt gtcgcccacg
     4081 ggctggcggc ggcgggggtc ccggtcgatt ccgcgccggc cctcggcacc tcggcgggtt
     4141 cctgggccgc cgcggtggtg gccctgggcg tcacctacga cgagatcgcc gggatcgacg
     4201 ccccgagggt ccccaaccgc cgaccggggg tcctggccga cgccgcggag tcggtgttcg
     4261 gcgcggccac ccacccgttg gtgtcggtct cggcggtctg cgtgcgcagc cgtcgacgtc
     4321 acatcctcga cggcgaccgc taccgcttgt cggacctggt ggcggcctcg tccgcggtgc
     4381 cgggactgct cccggcgcac cgggtcgacg gccggctcta cgtcgacggc ggagtccgtt
     4441 cggccacctc ggtcgacgcc gcgccggcgg cggagtcggt catcgtcgtc gctcctctcg
     4501 ccggcccgca cctgggggcg atggggagag gcgccggact gctgctccaa cgcgaggtcc
     4561 gggccttcag gcgccggtat ccggaccgtc ggatcaccgt catccgaccc gaccgggcga
     4621 tcgcggcgca cgccgggcgc aaccccatga acctcttcga cgcggaccgg gcccgggcgg
     4681 tctatccgct ggcggtcgat cagggtgtgc gttgcgccga gcgcctcgcc gcggcgccgg
     4741 tcgtctgacc tacgctccgt ccggcctcgg gtggtcgccc gcaggtgacg tcgtcccggg
     4801 ggtgtggcat ggatgggttc gagggcaagg tcgcggtcgt gaccggtggg gcgagcggga
     4861 tcggcgccgc catggccgcc gccttcgcgg cccgaggcat gaagctcgtg ctcgccgaca
     4921 tcgagcccgg gccgctcgag gagaccgccc acgccctccg cggcgacggt gcggacgtct
     4981 tcgccgtcac cgcggacgtg agccgggccg cggatgtcga gcgtgtcggc caggccgcga
     5041 tcgacacgtt cggagccctc cacgtggcct gcaacaacgc gggggtgggc agcggcggcg
     5101 ccacctggga ggtcgacctg tccacctggg agtgggtgct gggcgtgaac ctgatgggcg
     5161 tggtgcacgg cctgcggtca ttcacgccgc gcatcatcgc cagcggcggt ggccacatcg
     5221 tcaacaccgc gtcgatggcc ggtctgacat caccggcgtt catgagcccc tacaacgtgt
     5281 ccaagcacgc ggtggtgacc atgtcggagt cgatgtacgt cgagctcgag atgctgcacc
     5341 ccgaggtggg catcagcgtg ctgtgccccg gatgggtgca gacccgcatc aacgagtcgg
     5401 accgcaacca acccgatgac gtcgcggcgg cccaggccgc cgccgaggcc gccgacggcg
     5461 acgttccggt cgacgccgcc cagctcaagg cgatgatcga cgggttcgtg gccgagggga
     5521 tacgccccgc cgtcgtcgcc gacatggtcg tgagcgcgat cgaggagcga cgcttctaca
     5581 tcctcacgca cccggagtgg cagacgatgg tccgggaccg ggtggagcgg atgctgggag
     5641 gcgagaaccc gaccatggcg ctgccgggca actgatcacg ggcgcacggc cagcaccaga
     5701 ccgctgaccc gcgtcgctcc cgcctctcgc agcgtcgcgg ccgcggccga cagcgtcgcc
     5761 ccggtggtcc acacgtcgtc gaccaccacg acgtgtccgg ccaccggtgc ggtcgcacgg
     5821 aaggcggggc cgagcaaccg gtggtcgcgg tcggcgccgg tctgggcgcc ggcggagacc
     5881 cgctcgagca ccccggtcac cggtgtcgac gcccggcctc cgatgccgcg ggccagcagt
     5941 tcggcctgat cgaagccgcg ccggcgacgt cggcgggtgc tggtgggagc ccaggtgacc
     6001 gagttcaccg cgctggggga accggtcacg aagtgggcca gggcccggct gaccggcgac
     6061 aggacgtcgc ggtggttccg gaacttgagc tcggcgacca gccggggtgt ctccccgtcg
     6121 taggccagca gcgcccagca gtcgtccagc ccgacgggcg gctcccgatc gggtgccggc
     6181 gggagccggg acgtgcacgg tccgcacagg gcggagcccg gtgccgcgca cacggcgcag
     6241 cggcgggggg gcaggacctc gagcacggcc cgaccgtacg gcccggcccc gacaacggcg
     6301 ccggagccgg tgggcgcaag ggggtcgacc accgccaccg gtagagtggg cccgctatgg
     6361 gattcatgga tcgactgctg cgcaccggcg aggggaagaa ggtccgcgcc ctggcggacc
     6421 tggttcccga catcggagcg ctcgaacccg agatgcaggc cctctccgac gaggacctcc
     6481 agcaccgcac cgtcgagttc cgcgagcagc tcgatcgggg catcccgctc gacgacctgc
     6541 tgatcccggc cttcgccgtc gtacgcgagg ccgccagccg catcatcggc caacgccact
     6601 acgacgtgca gctcatgggt ggtgccgccc tgcacttcgg gtgggtcgcc gagatgaaga
     6661 ccggcgaggg caagacgctc gtgtcgaccc tgccggtgta cctcaacggc ctgcccggca
     6721 ccggggtgca cgtcatcacc gtcaacgact acctggcccg tcgcgacgcc gagtggatgg
     6781 gtcaggtcca ccggttcctc ggcctcacca tcggcatgat cctgcccggt gagcaggacc
     6841 cgaacttcaa gcgcgagcag tacgcgtgcg acatcaccta cggcaccaac aacgagttcg
     6901 gcttcgacta cctgcgcgac aacatggcgt tgtcgctcga ggagaaggtc cagcgcgggc
     6961 acgtctacgc catcgtcgac gaggtcgact cgatcctcat cgacgaggcc cgcaccccgc
     7021 tgatcatctc gggtcgggtc ggcgacgcgg ccaagctcta ctacaagttc gcgtcggtgg
     7081 cccgcggcct cacgcgtgac gtcgactacg aggtcgacga agagaagcgg gtcgtcttcc
     7141 cgctgcccga cggcatcgcc aaggtcgaga agagcctcgg tgtggagaac ctctacgacg
     7201 acgtctccaa cagcctcgtc caccagctgc aggtctcgat caaggccaag gagctctaca
     7261 agcgcgaccg cgactacctg gtgcagaacg gcgaggtgaa gatcgtcgac gagttcaccg
     7321 gtcgagtcct cgagggccgt cgctggtccg agggcctgca ccaggcggtc gaggccaagg
     7381 aaggcgtgaa gatcaaggag gagaaccaga ccctggcgac ggtcaccctc cagaactact
     7441 tccgcatgta cgacaagctc gccggcatga ccggcaccgc cctgaccgag gcggcggagt
     7501 tcatgtcgac ctacaacctg caggtcgtcc cgatccccac ccaccggccg gtgcagcgcc
     7561 gagacgacgc agacctgatc taccgcagcg aggcggccaa gttctccgcg gtggtcgacg
     7621 acatcgtcga gcgtcaggcc gagggtcagc cggtgctggt cggcaccgtg tcggtggaga
     7681 agtccgagcg cctcagccgc gagctcgaga agcgcggcgt tccgcactcc gtgctgaacg
     7741 ccaagcagca caccaaggag gccgaggtcg tcacccaggc cggccggatc ggctcggtca
     7801 ccgtcgcgac caacatggcg ggccgcggcg tcgacatcct cctcggcggc aaccccgagg
     7861 gcctcgcccg gcgcgaggtg cacgccgagg ggctcgagga cggcacggag gaggccgagg
     7921 cccgctacga ggagctgctc gccaagtacg aggtcgagtg caaggccgag ggcgaagagg
     7981 tccgcaagct cggcggcctg tacgtgctgg gcaccgagcg ccacgagagc cgccgcatcg
     8041 acaaccagct ccgcggccgc tccggccgtc agggcgaccc gggagccagc cggttctacc
     8101 tgtcgctcga ggacgacctc atgcgcttct tcgccaccgg ggcgatcgag tgggtcatgg
     8161 gcaagggctg ggacgacgac gtgccgatcg aggccaagat ggtcacccgg gccatcgaga
     8221 aggcgcagaa caccgtcgag gcgcgcaacg gcgagatccg caagaacgtc ctcaagtacg
     8281 acgaggtcat gaacgagcag cgcaaggtga tctacgcccg gcgcgacgag gtgctcgagg
     8341 gcgtcgacct gcgcgaggag accctccagg cgctggccga ggcgaccaac gagctgatcg
     8401 gcacccactg cggctcggac tacagcgacg actgggacat cgaggccctc ctcggcgaca
     8461 ccgccaccta ctggccggtc gagctcaccg tcgaggacct caccgaggct gcttcccagg
     8521 acggcctcta cgaccttctc atggccgacg ccgtcagcca ctacgagggg cgcgaggccg
     8581 agttcggcgc cgacacgttc cgtcagatcg agcgtcagat catgttgcgc atcatcgacg
     8641 ccaagtggcg ggaccacctc aaggagatgg actacctcca ggagggcatc aacctgcggg
     8701 cgctcggcca gaaggacccc ctcaccgagt ggcagcgaga gggcttcgac atgttcggct
     8761 cgctcatgga ggcggtcagc aaggaatacg tgcagtacgt gatgcacgtc caggcggtca
     8821 aggaggagca gccccgcctc cagccgcagc gcttcgacta ctccgctccc gacgacccgt
     8881 cctcggggcc gaacccggtg cccgccgtgg tcgccgcgga gtccgcccac gaggccgcag
     8941 ctgtccaggc cgacgacgac gccgccccgc agcagccggt ggtcaagagc gagtgggaca
     9001 agaccccgcg caacgcaccg tgcccgtgtg ggagcggcaa gaagttcaag gtctgccacg
     9061 gcgcggcctg agcccgaccg agcagcctga gcccgaccga acagacgaga acccacaccg
     9121 tgcaggactt caccccccga atcagcgcgc tccgcacccg actggccgag gccgagggct
     9181 acctgcgcgt cgccgagctg cgggcccgcc gtccacagct cgagaccgag ctcggtcggc
     9241 cggacctgtg ggacgagccc gagtccgccc aggccctgca gcgggagttc gccgagctga
     9301 gcgacgacct cgatctcttc gaccggctcg agtcccgcgt ggacgacgtc gacaccctgg
     9361 ccgagctggc ccgcgaggag ggcgacgagt cgctcgagcc cgaggtcgag tccgagctcg
     9421 ggtcgctggt gggcgagttc gatcgcctcg agctgcgggc gctgttcacc ggcgactacg
     9481 acgaccgcga cgccatcgtc gaggtcaact cgggcgccgg cggcgtcgac gcccaggact
     9541 gggccgagat gctgctgcgc atgtacgtgc gttgggcgga gcgctccggc atggacctgg
     9601 agatcgagtc gctcaacgag ggcaccgagg cgggcatctc ctcggccacc ttcctgctgc
     9661 ggggtcgcaa cgcctacggc tggatgcgct ccgagcacgg ggtgcaccgg ctggtgcgca
     9721 tctcgccgtt cgacaacaac gcccgtcgcc agaccagctt cgcctcggtg aaggtcaccc
     9781 cgtggatcga cgacgtcgac gacgagatcg acatcgacga caaggacctg cgcatcgacg
     9841 tgtaccgctc gtcgggcgcc ggcggccagc acgtcaacgt gaccgactcg gcggtgcgca
     9901 tcacccacct gcccaccggc atcgtcacct cgtgtcagaa cgagcggtcc cagcaccaga
     9961 acaaggaccg ggccatgaag atcctcaagg ccaagctgct cgacctggaa cgtcagaagc
    10021 gggccgagaa gctggccgag atcaccggcg agggcgcggc ggtcgacttc gggagccagg
    10081 accgcaacta cgtgttgcag ccctaccagc aggtcaagga cctgcggtcg ggcctggagc
    10141 tgtcgaaccc cgaggcggtg ctcgacggcg agttggacga cctgatggag gcctacctcc
    10201 agtgggtccg cgtcggcggc tgacccggtc acgtcgaccc cccctgcccg gaccccctgc
    10261 ccgttctgtt cgttggatca caacacgagt gttgtgatcc aacgaacaga acgggcgctg
    10321 gggtgtgggt gggcctccgc cacttgcggc gccggggctg agagactgct gccgacgtcg
    10381 gttcgcggcc ctccccgctg agctatcgtc acaggttcgt aacatcgccg cgggtcctcc
    10441 ctcccgcgga gagaggtcag cgtgatcaag ctcgagaacg tgaccaagat ctacaagggc
    10501 gagatggtcg cccttcgtga cgcttcggtc gacatcgaca agggcgagtt cgtcttcctc
    10561 gtgggtccct cggggtcggg caagtccacc ttcctgcgac tcctcaaccg cgaggagcga
    10621 cccgagaagg gccggatctt cgtcgccggc aaggacatcg ccgagctgcc gggctacaag
    10681 gtgccgtacc tgcgccgcaa catcggcagc atctaccagg acttcaagct gctgccgacc
    10741 aagagcgtct acgagaacgt cgccttcgcg ctcgaggcga tcggtcgacc ccgctccgcc
    10801 atccgcgagg ccgtccccca gatcctcgac ctggtcggac tggccaagaa gcagtcgaac
    10861 atgcccaacg agctctccgg cggcgagcag cagcgggtgt cgatcgcccg ggccttcgtg
    10921 aaccggccgc tgatcctgct cgcggatgag ccgaccggga acctggaccc ggccacctcg
    10981 gtgggcatca tgaagctgct cgaccgcatc aaccggacgg gcaccaccgt cgtcatggcc
    11041 acccacgacc gcggcatcgt cgacacgatg cgccgacggg tcatcgagct cgaccggggg
    11101 cgggtcgttc gcgaccaggc ccgcggcgtc tacgagtgag gcgacgtggc cgtacgcgct
    11161 gactacatcc tccgcgagac ggggtccaac ctcgtccgga acctgaccct cacgttcgcg
    11221 gcggtgctca ccgtggcggt gtcgctggcc atggtcgcgg ccagctacct gatcggtgcc
    11281 ggcatcaaca acagcttcct gggactgcgc tccgacgtcg acctgttcgt ctacatgaac
    11341 ccggcggcca ccgccgagca gatcaaggcg atggagaaga ccctcgactc cagtccccag
    11401 gtcggcgagt tcgacttcta cgacagcgcc cggtcctacg acgagttcaa gcgcttgttc
    11461 cgcgaccagc ccgacctggt caacacgatc aagccagagg acctgccgca gagcttccgg
    11521 atcaagccga agtcgaccga tgccgagatc gtctcgtcgc tcggcgacga gctcgaggcc
    11581 aaggagggcg tgctgcgcgt cgagtacgca gccgagtacg ccaaacggat gcagacctcg
    11641 gtgcggacgc tgaacacctg ggtgctgatc gtcggcggcg tgctgatcgc ggcctcggtc
    11701 ctgctgatct tcaacaccat ccgcaccgcg gtgttcgccc ggcggcgcga gatcgaggtc
    11761 atgcgactgg tcggggccag caactggttc atccgactgc cgttcatcgt cgagggcctg
    11821 gtccacggac ttctgggcgc ggcgttggcc gcggtggggg cgtggggctt cgaccagctc
    11881 tggaagcgca acttcgtcga ccaggtcggc ttcgagctgc tcaaccagat ccgctgggac
    11941 tccggtcagc tctggacctc gatcctgatc atcttcgtgg tcggcgccct gaccggtggc
    12001 atcggctcgg gcatcgcggt gagccggttc ctcaaggtgt gagccgggac gacaggggga
    12061 tgacggtggg ggacggctac cgcacgatcg gctacgacct cagcgacggc gtggccacga
    12121 tcacgctcga ccgaccggat cacatgaacg cgttcaccgt tcgcatgtgc ctggagatga
    12181 tcgacgcgtt cgaccgagtc gacgacgacc ccgatgtccg ggcggtgatc gtcaccggcg
    12241 cggggcgggc cttctgcgcc ggggcggacc tcgtcggcgg agcggacgcc ttcgacccgt
    12301 cgggatcggc cgcggccggg gcggcctcgg agctgggcga gggtgggggc gccccgcccg
    12361 gcggccccgc ggcctcggag ctgtcgccgg gggagcgcgc gatgcgccgg tccgtcgacc
    12421 cgcggcggga cttcggcggc cttctcacgc tgcgcatctt cgaggtcacc aagccggtga
    12481 tcgccgccat caacggcccg gcggtgggca tcggcgccac catgacgttg ccgatggaca
    12541 tccggctggc gtcggactcg gcgcggttcg gtttcgtctt cgcggcccgc gggatcgtcc
    12601 ccgaggccgc ctccagctgg ttcctgcccc gactggtcgg catccagcag gcgctcgagt
    12661 ggtgctacac ggccagggtg ttcccggcgg ccgaggcgtt gggcggcggg ttggtccgat
    12721 ccctgcacgc gcccgaggac ctgttgggtg cagcccgggc cctggcgacc gagatcgcca
    12781 ccaactcggc accggtgtcg gtcgctctga cccggcagct gatgtggagg ggcctggtgg
    12841 cggatcaccc gatggaggcc catcggatcg actcgccggg ggtcaccacc accggatcgg
    12901 gagcggacgc ccgcgagggg atcaccgcct tcttcgagaa gcgcccgccc gagtggcgcc
    12961 agcaggtgcc cggagatctg ccggactggt tcccgtggtg ggaccagccc tcgttcgacc
    13021 cctgagcgaa ccggccactg attcccggtc gggtgttgac ggacccgcac gggatcgatc
    13081 acgatatatc gcaaccgatc gagacagatc gagatgtccg gtcgatccag gccggcgaag
    13141 gaacgagagg ggccgcaacc gtgcgaccgg acaaccgcca catccctgac attcaccacg
    13201 acctcgacaa caaccaccat gatccgttcg gccgtcgggt tgggcgggga cgtcccatca
    13261 gaggcagcgg ccgcggtcgg ggtcggggcg gctaccgctc ccgctccggc ggccgcgcca
    13321 accggggcga cgtccgtgcc gcggtgctgg ccctgctcgc cgagcagtcg atgcacgggt
    13381 accagatcat cagcgagctc gatgagcgca ccggtggcgc ctggaagccg agccccggct
    13441 cggtctaccc gacgctgcag atgctggccg acgagggcct ggtccgatcc gagcccgagg
    13501 gcggcaagaa cgtgttccac ctcaccgact ccgggcgccg ggcggcagcc gagggtgact
    13561 ccgccccctg ggatgcggtg gccggcgacg gcgccaccgt cgatgtccgg cgcaccatcg
    13621 cccagctggc catggccttc aaacaggtca cccacaccgg gactgtggat caggtcgagc
    13681 gagccacccg gatcctcaac gatgcccgca aggcgatcta cggcatcctc gccgaggacc
    13741 cgtccgagac caggtcctcc tgaaccgcac catggtgccg gtcgggatgc ccgcctcttt
    13801 caggccgcgg cgccggcgag cacgaggtcg accagatcga cgacccggcg cgggcccagg
    13861 cccgcgttgc gcatgagcag cccgtagatg accgcgccga ccagggcnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc cgcggccacc gcggcgacga cagctccgcc
    14041 gtgcatcgcg tcgacgaagg cgttgcgggc gagctgggcc agtccgtcac cggcatcacc
    14101 ggcccgttcg gccaccgcca gagcaccacc cacgccgctg cgagcggcgt cgagcgcctg
    14161 gtcggggacc ttgcccgaca gcgcctcgac gatcttcgat ccgtacaccg aggacagcac
    14221 gctgccgatg atggcgacgc cgagagcacc gcccacctgg cgggtcgtgt cgttcatggc
    14281 cgagccgacg ccggccttgg ccagagggag ggagcccatg atcgactctg ttgccggcgc
    14341 catggtcatg cccatgccca tggcgagcag catcatccgc cacatcaccg ggaggtacgg
    14401 actctcggcg gtcagctgca gctgcagcaa cagggagatg gtgaccagcg tcaatccggt
    14461 ccccaccacg accttggtcc cgaccttctc gacgatccgg ctggacagcg gtgcgacgac
    14521 catcagcgtg agggccaggg gcagcaggcg cacgccggtc tcggcggggg agtaccccag
    14581 tacgaactgg aagtactggg tctgcaggaa cgagaagccg aacagggcga agaacaccag
    14641 ggtgatcgcg gtggacgccg cggtgaagcg ggggttcttg aagaaggtga cgtcgaacat
    14701 cggctcggcg gagtgcagct cgtggacggc gaacagggtc aggagcaccg cggcggcggc
    14761 gaagcagccg aggatgaccc cgtcggtcca cccccggccg ggagcctcga tgaggccgaa
    14821 caccagcgtc acgagcccgg cgatcgacag gactgctccg agcgggtcga gccggggtgc
    14881 cgacgggtcc ttggagcgtg ggagcagcac gaccccggcc ccgatcgcga cgacggcgat
    14941 cgggatgttg acggtgaaca ccgcgtgcca gctgaagtgc tcgaccaggt agccgccgag
    15001 cagcgggccc agggcgatgc cgagccccga tacgcccgcc cacacgccga tggcccgtcc
    15061 gcgctcctcg gcggggaaca cgttggtgag gatcgacagc gtggcgggca tgatgaacgc
    15121 cccgccgatc cccatcagcg atcgggtcac gatcaggtgg ttcgggtcgg tggcgaacgc
    15181 ggagagggcc gagccgactg cgaacacgac catgccgatc tgcagggcgc ccttgcgacc
    15241 gaaccggtct cccaggctgc cggcggtgag cagcagcccg gcgaacacca gggtgtagct
    15301 gtcgacgatc cactgcagct ggctgtcggt cgcaccgagg tcccgcacca gggtgggcag
    15361 ggcgacgttg aggatggtgt tgtcgagcgt gatcaccatc agcgacaggc acagcacgcc
    15421 gaggatccac caccggcgtc ggtacacgag ctcttcggac atgagggg
//
LOCUS       DAOQOD010000069        15417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1366_length_15417_cov_11.471879, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000069 DAOQOD010000000
VERSION     DAOQOD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15417
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_15417_cov_11.471879"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..372)
                     /locus_tag="PLZ72_08400"
     CDS             complement(4..372)
                     /locus_tag="PLZ72_08400"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNA36354.1"
                     /translation="MSSPDRYDHRRLGVYGEDRAQRWYLERGYVLLDRNWRCPDGELD
                     LVLGRRDGGGSTIVFCEVKTRTNHRFGSPFEAVGRDKQRRLRQLALRWLDAHDVRVRH
                     LRFDVAAVTSGRIEVLTDAF"
     gene            complement(460..804)
                     /gene="rplS"
                     /locus_tag="PLZ72_08405"
     CDS             complement(460..804)
                     /gene="rplS"
                     /locus_tag="PLZ72_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNA36355.1"
                     /translation="MKTTDLIDQASLRTDIPDFAPGDTLKVHVKVIEGNKERVQVFQG
                     AVIARQGGGLQETFTVRKLSYGVGVERTFPLHSPIIDRIELHSRGDVRRAKLYYLRDR
                     VGKAAKIKEKRD"
     gene            complement(922..2553)
                     /locus_tag="PLZ72_08410"
     CDS             complement(922..2553)
                     /locus_tag="PLZ72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNA36356.1"
                     /translation="MSRTVRVAVVAAVVALLGAACTSTEDSSSRDDGPAASSTTVVRE
                     CTPDRPTEVVAAPVTGSTHDLDITSFDGTTIRAHWFPVEGATDVPTVLMGPGWGMAGD
                     TDVDAVGVLGAVNIAVLRDAGYNVLTWDPRGFGASGGTAQVDSPDHEGRDVQVLLDWV
                     AAQPEVRTDADGDPAVGMVGGSYGGGIQLVAAAIDCRIDAIVPIVAWHSLVSSLYPRE
                     TFKQGWADVLSAVGARAHLDPMVAAANEQGRRVGVLTAEQIDWFAARGPADRVSEITA
                     PTLLVGGTVDTLFPLAEDVANYRALRGAGTDVSMFWFCGGHGTCLADKGDEQLMVRRV
                     VAWLDRWVKRDPTVDTGPRIDVIDQYGRRHVADDYPGVAGEPLIGRGSGTLELAAGGG
                     SGPATIPPGKADVLAGVAGRLIPAPASRAIEVEVPVPADSLALGAPRLTLTYTGTAPA
                     GDRPTRVFAQLVDPASGLVIGNQIAPVPVVLDGSEHRSTVDLEVISHRVTEGSPLLLQ
                     LVATSTLYLEPLLGGRIEVSKAAIEVPTATGYRAG"
     gene            complement(2550..3308)
                     /gene="trmD"
                     /locus_tag="PLZ72_08415"
     CDS             complement(2550..3308)
                     /gene="trmD"
                     /locus_tag="PLZ72_08415"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNA36357.1"
                     /translation="MRIDVITIFPAMVRDFAGASLLGKAQERGLLDLRVHDPRDVTTD
                     VHRTVDDAPFGGGAGMVLTPGPLFDIVEREQPPRPLFLLGPGGRTLDQPLARELAALD
                     GFSLLCGRYEGVDDRVRSHLVDGEISIGDVVLAGGEVAAMVVLEAVGRLVPGVMGNDT
                     SADEESFSDGLLEHPQYTRPAQFRDWDVPDVLRSGDHARVARWRRATALRRTMDHRPD
                     LIDARGGLSDEDRRVLVEFSMLRPDDERPPDADR"
     gene            complement(3323..3811)
                     /gene="rimM"
                     /locus_tag="PLZ72_08420"
     CDS             complement(3323..3811)
                     /gene="rimM"
                     /locus_tag="PLZ72_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNA36358.1"
                     /translation="MTPDDGPRMLEVGRIAKAHGLNGEVNVVLVSNRPERLDPGSVLS
                     TDRGELTVVSSRRHCDRWLVRFAGHDDRTGAEALRGLELRAEPIEDPDELWVHELIGC
                     RVLSVDGVDRGPVIAVQENPAADLLVLESGALVPVVFVVDGPVDGTVRVEVPDGLFEL
                     GS"
     gene            complement(3808..4098)
                     /locus_tag="PLZ72_08425"
     CDS             complement(3808..4098)
                     /locus_tag="PLZ72_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNA36359.1"
                     /translation="MSDESIDDGVDTDEGPRPTHAAAVLEHVAKSLVDVPDEARVDVD
                     DKGRRTQVNLRVADGDMGRIIGKRGRMANAIRTVTRAAAAHDGVEIDVEFLD"
     gene            complement(4095..4367)
                     /gene="rpsP"
                     /locus_tag="PLZ72_08430"
     CDS             complement(4095..4367)
                     /gene="rpsP"
                     /locus_tag="PLZ72_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNA36360.1"
                     /translation="MAVRLRLMRMGKKKQPTYRIVAADSRSPRDGRFIEIVGTYDPRR
                     EPSAVTVDNDKAVDWLQKGAQPSERVQKLLEISGAWEQFESSKAKK"
     gene            complement(4568..6043)
                     /gene="ffh"
                     /locus_tag="PLZ72_08435"
     CDS             complement(4568..6043)
                     /gene="ffh"
                     /locus_tag="PLZ72_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNA36361.1"
                     /translation="MFESLSDRFDGIFTRLRGKGRLSEADVDEVLREIRLALLEADVN
                     LGVVKAMLARIRERAIGEDLSQALNPALQVKKIVLEELTTTLGGETLRITYASKPPTI
                     VLMAGLQGSGKTTNSAKLARWFKAQGRNPILVGADLQRPAAVQQLRTLGAQIDVPVWS
                     AEDDVVASGLGDPVAVARGAVDEARRRGRDVLIVDTAGRLAIDAEMMEQVRQISDSLE
                     PDYTFLVVDAMTGQDAVTVAESFHQTLELDAVILTKLDGDARGGAALSVKEVVGRPIA
                     FASTGEKIEDFDLFHPDRLAGRILGEGDLATLIEKAETAFEQEEAEAAAARLLDGTFT
                     LDDFLEQMQALKKMGPLSGVMSLLPGVPKDIRDVEIDDRQIAHVEAIIKSMTPAERVE
                     PDIVDGSRRARIAKGSGTSPNEVTQLITQFKQMRQMMKDMGKAPRRKKGGRGKGSKKA
                     KKGGRVTAKGPAAVNKAAFSLPTLEEMEAQLPKGGGFPKLG"
     assembly_gap    6087..6186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6187..6890
                     /locus_tag="PLZ72_08440"
     CDS             <6187..6890
                     /locus_tag="PLZ72_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNA36362.1"
                     /translation="AVEPDMRIEIWSDVVCPWCYLGARRLTAAIGQFVEAYPDARPTV
                     HFRAYELDPGAPAEPRPMRPVIERKYGPGAFDSMTARLTALGVAEGIEYRFEDTLRVN
                     TFDAHRLLAWAGAIDDGSLEGSSIDHDGGSGVQQRLSDALFRAYFTEGRNVADRVTLI
                     EICRQAGLDPEAAAEVLESGGFAEEVRTDEETARDLDITGVPATVVDGRLLVPGAQEV
                     ATFARVLERAAASVR"
     gene            6938..8089
                     /locus_tag="PLZ72_08445"
     CDS             6938..8089
                     /locus_tag="PLZ72_08445"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR02326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HNA36363.1"
                     /translation="MAGHQHTDDSGAAPPVLLTPGPLTTSERTRRAATVDWGSWDRRF
                     NDLTASVRHRLAAASGAARPVLCVPMQGSGTFAVEAAVRTLVAPGDRLVVAVNGTYGA
                     RIADMATRAGRRAVVVEASTDVPVPVDAVADAFEQHPDARHLAVVHCETSTGLLNPVE
                     DLADVAHQMGRALIVDAMSSFGVLDTAIDHPAVHAVVAASGKCLEGLPGMGFVLVPPD
                     VLDAAAGRCDSLSLDLVDQHRYMERTGQWRYTPPTHVVAALHEALLQYEEEGGAPVRL
                     DRYRANRGALDAGMAALGFRHYLHESLRTPIIQTFHSPDHPSWSFPTFYELVKQRGFV
                     LYPGKLTTEETFRVGCIERSRPPSSPRRSAPSSRLWTQWASPSLFHRAD"
     gene            8139..8816
                     /locus_tag="PLZ72_08450"
     CDS             8139..8816
                     /locus_tag="PLZ72_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonatase-like hydrolase"
                     /protein_id="HNA36364.1"
                     /translation="MRTEIVVMDMAGTTVSDDGLVVAAFTRAVASLGIDEDHERFPAM
                     MDYVNTTMGESKITVFRTLFDGDDDMAQRANAAFEHAYGDLVAEGRATALPGAAETIA
                     TLRGRGLKVALTTGFSPVTRDAIIDSLGWRDHVDLALSPADVGRGRPFPDLNLSALLR
                     LGGTDVRALATVGDTAFDVLAGRASGAGVVAGVLSGAHDRDTLSAAGADHVIDTVADL
                     PDLLANL"
     gene            complement(9052..10326)
                     /gene="ftsY"
                     /locus_tag="PLZ72_08455"
     CDS             complement(9052..10326)
                     /gene="ftsY"
                     /locus_tag="PLZ72_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNA36365.1"
                     /translation="MEPLVILAVILALVAVLAVVAGATLVSRRGRGETLEPPPEVDLE
                     QIDEAGPGAVAPREAPTPSDRDTLEEPEVEVVEPRGAMFGGTVEITGPELTEEEEAAN
                     IAAAEAAEAEWESEQAGGVEELVRPSFRDRLSRARSAFSGYVGSIMSRSTIDEETWDD
                     LEEALIRADVGIGPAQELLDAVRSTVKERGLTTPDELIDAVKDEMKARLAGSVELHVA
                     ETAPTVWLFVGVNGVGKTTTIGKLGRRLADQDRTVVMAAGDTFRAAAAEQLTMWADRC
                     GADIVRGAEGGDPSSIIFDAIASASAKGADVVLADTAGRLHTKSNLMDELAKVRRVAD
                     KGDGTVTEVLLVLDATTGQNGLQQARQFTEATDVTGVVLTKMDGSAKGGIVFAIRSEL
                     GIPIKLVGLGETAADLVDFDADEFIDALFAEE"
     gene            10525..11616
                     /locus_tag="PLZ72_08460"
     CDS             10525..11616
                     /locus_tag="PLZ72_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA36366.1"
                     /translation="MDQPSLTTPTPAAEIERLLFELKKVVVGQDRAIERVLVCLIAGG
                     HCLLESVPGLAKTLMVETLASSVGGSFSRIQFTPDLLPADLVGTRIYRASSEQFDVEL
                     GPVFANFVLADEINRAPAKVQSALLEVMAEHHVTIGGQRFDAPEPFLVLATQNPIESE
                     GVYPLPEAQRDRFLMKVLLDYPAPAEEVEIVQRMGTNPPHAEQALTLAQVEGLQAAAE
                     EVFVDRSVLEYAVNLVLCTRTPENFGLPELRTFIELGASPRASLGLIRGGRALALLRG
                     RNYLTPQDVFDVAPEIMRHRMLLSYEALAQDVDVEQVLARVLGTVPAPRLSPTQGGGD
                     AYYGTSTDAGAWNAPAASGAAAAWGNGAS"
     gene            11613..12557
                     /locus_tag="PLZ72_08465"
     CDS             11613..12557
                     /locus_tag="PLZ72_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA36367.1"
                     /translation="MSGLSHTLGLVGDRPAEEILRRLTLDVNRKLDGMLHGDFLGLVP
                     GRGTEPGETRAYSPGDDVRRIDWNVTARMQDPYIRETIADRELEAWLVVDRSARLDFG
                     TATCEKRDLALAAAAGVGLLTGRGGNRVGALILRGSELTTVPPRNGRTHLLGILERIM
                     STPRGDGSGTSDLAATLKRAGAIAPRRGLVVVISDFLDDPDRWRTALGTVALRHAVLC
                     VEVVDPRELELPPVGLVQFRDPATGATREVNTNDSGTRARYAEAAARQRATIAGAIRG
                     AGADHLQLRTDRDWILDLARYVSRRRRRAELAATAAPR"
     gene            12618..13589
                     /locus_tag="PLZ72_08470"
     CDS             12618..13589
                     /locus_tag="PLZ72_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA36368.1"
                     /translation="MTDWFLHPERLWLILVVVGLAALYVGLQFTSPRYAVRFSNLELL
                     DSVAPRRPGWRRHVVAGCFLVAAAVLVAAFAQPVMTEKVPSERSTIVLAIDTSLSMAA
                     TDVSPNRIEAAQAAAKEFIDKLPDQLNVGLISFAGSAQLLVPPTQDHAKVNAAIDGLE
                     LDKSTAIGDATKLAVQVIEDQARGTDGKKVDGAVVVMSDGETTVGLPTADAIPIAKKA
                     GVAITTIAYGTQEGEITVDTDGDGVGQRTRVPVNVEELRSLAEGTGGTAYTAETAQSL
                     RSVYSELGSTIGYDEKSQDVTYRFVGLSLVLLLVAAGFSLRWFSRLP"
     gene            complement(13645..>15417)
                     /locus_tag="PLZ72_08475"
     CDS             complement(13645..>15417)
                     /locus_tag="PLZ72_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA36369.1"
                     /translation="TVGVGEPVRRHVRSAAGAGAASAAIDALLDALLDGAVVVDGDLD
                     AALSASLSSPSAVVVTRRGDRFAAAGWRLRAAGSGATGAALDEARDRAEQAGSERDRA
                     RRGVESARESVAATRDAEQQAARRRDRAAADRAALADKAARLDHDAQEAAGELEALGA
                     HLQELVDRLAREEVRVAELSGQLPALEAAEAELGERARAMAEARERLDARGADVQARR
                     TDLEVRIAALDERRRYVGSRLEQIGERLARNQAEVAAAADRRIVLEARLVATDRLHSF
                     LSDRLSRIEPVLAELRQERRRQSEATREVTNLLDGLRSERSTSERSLEETRARLQKAE
                     ISDAESRMRLDTAVESLRHDLDTEPDAAMAAECPSLPEGVSAPARVRELERELRLMGP
                     INPLALQEFDELSERHTFLESQLEDIRQTRRELSKVIKAVDEEIVSVFSAAYADVSQN
                     FTLLFETLFPGGQGRLRLTDPDNLLETGIELEARPSGKNVRKLSLLSGGERSLTALAY
                     LFAVFRSRPSPFYVMDEVEAALDDVNLHRFLDLIAEFRDAAQLIVVSHQKRTMEAADV
                     LYGVTMEPAGSSRVVSERAATVLD"
BASE COUNT         2215 a         5558 c         5336 g         2208 t
ORIGIN      
        1 cgctcagaac gcgtcggtca ggacctcgat ccgaccgctg gtgaccgcgg cgacgtcgaa
       61 gcgcagatgg cggacacgaa cgtcgtgggc gtccaaccac cgcaacgcca actggcgcag
      121 ccgtcgctgc ttgtcccgac caacggcctc gaacggagat ccgaaccggt ggttggttcg
      181 ggtcttgacc tcgcagaaca cgatggtcga cccgccgccg tcgcgtcggc cgaggaccag
      241 gtccagttcg ccgtcggggc agcgccagtt ccggtcgagc aggacatagc cccgctccag
      301 gtaccaccgc tgggcgcggt cctcgccgta gacgccgagg cgccggtggt cgtagcggtc
      361 cggtgacgac atggttcccc ctcgccggac gcgccggcgg tcggtgtgga gggggaaggc
      421 gatcgggcgc tcaacccaga gcgaccggag aggcgacgat cagtcgcgct tctccttgat
      481 cttggcggcc ttaccgacgc ggtcgcgcag gtagtagagc ttggcccgac ggacgtcgcc
      541 gcgggagtgc agctcgatgc ggtcgatgat cggcgagtgc agcgggaacg tgcgctcgac
      601 gccgacgccg taggacagct tgcggaccgt gaaggtctcc tgcagaccgc cgccctgacg
      661 agcgatcacc gctccctgga agacctggac acgctccttg ttgccctcga tcaccttcac
      721 gtgcaccttc agggtgtcgc ccggggcgaa gtcggggatg tcggtgcgga ggctggcctg
      781 gtcgatgagg tcggtggtct tcattcggga gatctcctgg gtggcgccgt ggggaacggg
      841 ccgcgtcgga cggccgctgg cgcgcagcgg gccgatgata gtgcctgcag ctcgcgacgg
      901 tgaaacccca ccggtcgcac ctcagcccgc gcggtagccg gtcgcggtgg gcacctcgat
      961 ggcggccttc gacacctcga tgcggccacc cagcaggggc tccaggtaga gagtcgaggt
     1021 ggcgacgagc tggaggagca gcggggaccc ctcggtgacc cgatgcgaga tcacctcgag
     1081 atcgacggtg ctgcggtgct cgctcccgtc gaggacgacc gggaccggcg cgatctggtt
     1141 cccgatcacc agcccgctcg ccgggtcgac gagttgggcg aagaccctgg tcggtcgatc
     1201 cccggccgga gcagtccccg tgtaggtgag ggtgagtcgg ggagccccga gcgccaggga
     1261 gtcggccggc accggcacct ccacctcgat cgcccgtgac gccggtgccg ggatcagccg
     1321 tccggccacc ccggcgagca cgtcggcctt cccgggtggg atcgtcgcgg gcccggatcc
     1381 gcctccggct gccaactcga gggtgcccga acccctgccg atgagcggct ctccggcgac
     1441 cccggggtag tcgtcggcga cgtgtcgtcg gccgtactgg tcgatgacgt cgatccgggg
     1501 tcctgtgtcc accgtcggat cacgcttcac ccagcggtcg agccacgcca cgacacgacg
     1561 gaccatcagt tgctcgtcgc ccttgtcggc caggcaggtg ccgtggccgc cgcagaacca
     1621 gaacatggac acgtcggtgc cggcgccgcg aagcgcccga tagttggcga cgtcctccgc
     1681 cagcgggaac agggtgtcca ccgttccacc gaccaacagg gtcggagcgg tgatctcgga
     1741 gaccctgtcc gccggccccc gggccgcgaa ccagtcgatc tgctccgcgg tcagcacccc
     1801 gacgcgtcgg ccctgttcgt tggccgccgc gaccatcggg tcgaggtggg cgcgggcgcc
     1861 caccgcgctc aggacatcgg cccagccctg cttgaaggtc tcccgcgggt acagcgacga
     1921 caccagcgag tgccatgcca cgatcgggac gatcgcatcg atgcggcagt cgatcgccgc
     1981 ggcgaccagc tggatgccgc cgccgtagga gccgccgacc atccccaccg ccgggtcgcc
     2041 gtcggcatcg gttcgcacct cgggctgggc tgccacccag tcgaggagga cttggacgtc
     2101 gcggccctcg tggtccggcg agtcgacctg ggccgtcccg ccggaagccc cgaagcccct
     2161 cgggtcccag gtcaggacgt tgtagcccgc gtcccgcagc accgcgatgt tgaccgcgcc
     2221 gagaacgccg accgcgtcca cgtcggtgtc acccgccatc ccccagcccg gccccatgag
     2281 caccgtcggg acgtccgtgg cgccctccac ggggaaccag tgggcccgga tggtggtgcc
     2341 gtcgaacgac gtgatgtcga ggtcgtgggt ggaaccggtg accggggccg ccaccacctc
     2401 ggtcggtcga tcgggtgtgc actcgcggac aacggtggtg ctcgacgccg cgggcccgtc
     2461 gtcacgcgag gacgaatcct cggtgctcgt gcacgccgca cccaacagcg ccaccaccgc
     2521 ggcgacgacc gcgacccgca ccgtccggct catcggtccg cgtccggcgg tcgctcgtcg
     2581 tcgggccgta gcatcgagaa ctccaccagg actctccggt cctcgtcgct cagcccgcct
     2641 cgggcatcga tcaggtcggg gcggtggtcc atggtgcggc gaagcgccgt cgcccgacgc
     2701 cagcgggcga cccgggcgtg atcaccggac cgcagcacat cgggcacgtc ccagtcccgg
     2761 aactgcgccg gccgggtgta ctgcgggtgt tccagcagac cgtcggagaa ggactcctcg
     2821 tccgcggacg tgtcgttgcc catcaccccc gggacgaggc ggccgaccgc ctcgagcacg
     2881 accatcgccg ccacctctcc cccggccagc acgacgtcgc cgatcgagat ctcgccgtcg
     2941 acgagatggc tgcggacccg gtcgtcgact ccttcgtagc gaccgcacag cagagagaac
     3001 ccgtcgagtg ctgcgagctc ccgggccagg ggctggtcca gggtccgacc gcccggaccg
     3061 agcaggaaca gcggtcgcgg cggctgctcc cgttcgacga tgtcgaacag ggggcccggg
     3121 gtgaggacca tgccggctcc cccaccgaag ggggcgtcgt ccacggtgcg gtgcacgtcg
     3181 gtggtcacgt cgcgggggtc gtgcacccgg aggtcgagca gaccccgctc ctgggccttg
     3241 ccgagcaggc tggctccggc gaagtcccgg accatcgccg ggaagatggt gatgacgtcg
     3301 atgcgcacgg agatcgaccg ggtcaggaac cgagctcgaa gagcccgtcg gggacctcga
     3361 ctcgcaccgt gccgtcgacg ggaccgtcga cgacgaacac gaccgggacg agggcaccgg
     3421 actcgagcac cagcagatcg gcggccgggt tctcctgcac cgcgatgacc gggcctcgat
     3481 cgaccccgtc gacgctgagg acccgacatc cgatcagctc atggacccag agctcgtcgg
     3541 gatcctcgat cggctcggct cgcagctcca gcccccggag cgcctcggct ccggtccggt
     3601 cgtcgtggcc ggcgaagcgc accagccagc gatcgcagtg gcggcgggac gagacgacgg
     3661 tcagctcccc gcggtcggtg gacagcaccg agcccggatc gagccgttcg ggccggttgg
     3721 acaccaggac gacgttgacc tcgccgttca ggccgtgggc cttggcgatc cgaccgacct
     3781 cgagcatccg gggtccgtcg tcgggcgtca atccaggaac tcgacgtcga tctcgacgcc
     3841 gtcgtgggcc gcggcggcac gggtgacggt gcggatggcg ttggccatgc gaccgcgctt
     3901 gccgatgatg cgacccatgt cgccatccgc gacccgcagg ttcacctggg tgcggcggcc
     3961 cttgtcgtcg acgtcgaccc gggcttcgtc cggcacgtcg acgagggact tcgcgacgtg
     4021 ttcgagcacg gccgccgcgt gtgtggggcg cggaccctcg tcggtgtcga cgccgtcgtc
     4081 gatcgactcg tcgctcactt cttggccttg gaggactcga actgctccca ggccccggag
     4141 atctccagca gcttttggac ccgctcggag ggctgggcgc ccttctggag ccagtcgacg
     4201 gccttgtcgt tgtcgacggt caccgccgag ggctcccggc gggggtcgta ggtgcccacg
     4261 atctcgatga accgaccgtc gcggggggaa cgggaatcgg cggcgacgat gcggtaggtg
     4321 ggctgcttct tcttgcccat ccgcatgagg cggagtcgaa cggccacgga gttgtccttc
     4381 ttcttgttcg gaggggtcgg gtgtcggctt gtgggtccga tccgatccac cggggtgggt
     4441 cggatcgggc gaaccggtct ggtcgggcgc acggcacgcg cagcgacgca gggcagcggt
     4501 ccatcttggt cgcgccgggc gacggaggca agctctccgg gaccgaccgg tgcctcggac
     4561 ccgggcctca accgagcttg gggaaccctc cgcccttggg gagctgggct tccatctcct
     4621 ccagcgtggg cagcgagaac gctgccttgt tgaccgcggc cggccccttg gcggtgaccc
     4681 ggccaccctt cttcgccttc ttggagccct tgccgcgacc gcccttcttg cggcgcggcg
     4741 ccttgcccat gtccttcatc atctggcgca tctgcttgaa ctgggtgatg agctgggtca
     4801 cctcgttggg agacgtgccc gagcccttgg cgatccgggc ccgccgcgag ccgtcgacga
     4861 tgtcgggctc gacacgctcg gccggggtca tcgacttgat gatcgcctcg acgtgcgcga
     4921 tctggcggtc gtcgatctcg acgtcgcgga tatccttggg gacgcccggc agcagcgaca
     4981 tcacccccga cagcgggccc atcttcttga gcgcctgcat ctgctcgagg aagtcgtcga
     5041 gggtgaaggt gccgtcgagc agccgggccg cggcggcctc ggcctcctcc tgctcgaagg
     5101 cggtctcggc cttctcgatc agggtggcca ggtcgccttc gcccaggatc cgccccgcca
     5161 agcggtcggg gtggaacagg tcgaagtcct cgatcttctc gccggtggac gcgaaggcga
     5221 tcggacggcc caccacctcc ttgaccgaca gggcggcgcc gccgcgggcg tcgccgtcga
     5281 gcttggtcag gatgacggcg tcgagctcga gggtctggtg gaacgactcg gcgacggtga
     5341 cggcgtcctg gccggtcatc gcgtcgacga ccaggaacgt gtagtccggc tcgagcgagt
     5401 ccgagatctg acggacctgc tccatcatct cggcatcgat cgccagtcgt cccgcggtgt
     5461 cgacgatcag gacgtctcgg cccctccgtc gggcttcgtc gaccgcgccg cgggccacgg
     5521 ccaccggatc gcccagaccc gaggcgacga cgtcgtcctc ggcagaccac accggcacgt
     5581 cgatctgggc cccgagcgtc cgcagctgct ggaccgcggc aggtcgctgg aggtcggcgc
     5641 cgaccaggat ggggttgcga ccctgcgcct tgaaccagcg ggcgagcttg gccgagttgg
     5701 tcgtcttgcc cgacccctgc agaccggcca tcaggacgat cgtcggcggc ttcgacgcgt
     5761 aggtgatgcg cagcgtctcg ccgccgaggg tggtcgtgag ctcctcgagg acgatcttct
     5821 tgacctgcag cgccgggttc agcgcctggg agaggtcctc accgatggcc cgctcgcgga
     5881 tccgggcgag catcgccttg accacgccca ggttgacgtc ggcctcgagc agggccagtc
     5941 ggatctcgcg gaggacctcg tcgacatcgg cctcggagag ccgtcccttg ccgcggagcc
     6001 gggtgaagat gccgtcgaaa cggtcggaga gtgactcgaa catcgggggg ccacgctacc
     6061 ggcgagcggg ctcacccggg caaccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnngagc ggttgaaccc gacatgcgca tcgagatctg gtccgacgtc gtgtgcccgt
     6241 ggtgctacct gggtgcccga cggctcaccg ccgcgatcgg gcagttcgtc gaggcgtacc
     6301 ccgacgctcg gccgacggtc cacttccggg cctacgagct tgaccccggt gcgccggcgg
     6361 agccccggcc gatgcgtccg gtcatcgagc ggaagtacgg accgggggcg ttcgactcga
     6421 tgacggcgcg gttgaccgcg ctcggggtgg ccgagggcat cgagtaccgc ttcgaggaca
     6481 cactccgggt caacactttc gatgctcacc gcctgctcgc atgggccggg gcgatcgacg
     6541 acgggtcgct cgaggggtcc tcgatcgacc acgacggagg gtccggggtg caacagcgcc
     6601 tctccgacgc gctgttccgg gcgtacttca ccgagggtcg caacgtcgcg gaccgcgtca
     6661 ccctgatcga gatctgccga caggccgggc tcgatccgga ggcggccgcc gaggtgctgg
     6721 agtcgggggg cttcgccgag gaggtccgca ccgacgagga gacggcccgc gacctcgaca
     6781 tcaccggcgt ccccgccacc gtcgtcgacg gacgcctcct cgtgcccggc gcccaggagg
     6841 tcgccacctt cgcacgggtg ctggagcggg ccgcggcgtc ggtgcgctga tcgtctccca
     6901 gccgatccgg tcggtccgct cccctagcct gcgagacgtg gcgggccacc agcacaccga
     6961 cgacagcgga gcggcgccgc cggtcctgtt gacccccggc ccgctcacga ccagcgagcg
     7021 gacccgccga gcggccaccg tcgactgggg gtcgtgggac cgacggttca acgacctgac
     7081 cgcgtcggtg cgccaccggc tggcagcggc ttcgggagcg gcacgaccgg tgctgtgcgt
     7141 accgatgcag gggtcgggga ccttcgcggt ggaagccgcg gtgcggaccc tggtcgcgcc
     7201 gggcgaccgg ctggtcgtcg cggtcaacgg cacgtacgga gcacggatcg cggacatggc
     7261 cacccgggcc ggccgccggg ccgtcgtggt cgaggcctcg accgacgtcc cggttcccgt
     7321 cgacgcggtg gccgacgcct tcgagcagca ccccgacgcc cgccacctcg ccgtcgtgca
     7381 ctgcgagacg tcgaccggtc tcctgaaccc ggtcgaggac ctggccgacg tggcccacca
     7441 gatggggcgg gctctcatcg tcgacgccat gagcagcttc ggcgtgctcg acaccgcgat
     7501 cgaccaccct gcggtgcacg ccgtggtcgc cgcgtcgggc aagtgcctgg agggcctgcc
     7561 cggcatgggg ttcgtcctgg tgccgccgga cgtcctcgac gcggcggcgg gccgctgcga
     7621 ttcgctgtca ctcgacctgg tcgaccagca ccgctacatg gagcggaccg gtcagtggcg
     7681 ctacaccccg ccgacgcatg tggtcgcggc gctgcacgag gcgctcctgc agtacgagga
     7741 ggagggcggg gcgccggtcc gactggaccg ctaccgcgcc aaccgcgggg cgttggacgc
     7801 cgggatggcc gccttggggt tccgccacta cctccacgag tcgctgcgga cgccgatcat
     7861 ccagaccttc cactcccccg atcacccgtc ctggtcgttt ccgaccttct acgagctggt
     7921 gaagcagcgg ggcttcgtgc tctatccggg caagttgacc accgaggaga cgttccgggt
     7981 cggctgcatc gagcgatcac gcccaccgtc ctcaccgcgg cggtcggcgc catcgagtcg
     8041 gctctggacg caatgggcat ccccgtccct cttccaccga gctgactgaa ccgcaccgcg
     8101 gtcgggtgac ccggccgaca gaccgacagg aggcaccgat gcgcaccgag atcgtggtca
     8161 tggacatggc cggcaccacc gtgtccgacg acgggctggt ggtcgccgcc ttcacccggg
     8221 cagtggcttc gctcggcatc gacgaggacc acgaacgctt cccggccatg atggactacg
     8281 tcaacaccac catgggcgag tccaagatca ccgtgttccg gaccctgttc gatggcgacg
     8341 acgacatggc ccagcgagcc aacgccgcgt tcgaacacgc ctacggcgac ctggtcgccg
     8401 agggtcgggc cacggctctt ccgggcgcgg ccgagaccat cgcgacgctc cgcggtcggg
     8461 ggctcaaggt ggcgctgacc accgggttct ctcccgtcac ccgcgacgcc atcatcgact
     8521 cgctgggctg gcgcgaccac gtcgacctgg cgttgtcgcc ggcggacgtc ggacggggtc
     8581 gaccgttccc ggacctgaac ctctcggccc tgttgcgcct gggcggcacc gacgtgcggg
     8641 cgctggcgac ggtcggcgac accgcgttcg acgtcctcgc cggtcgggcc tccggggccg
     8701 gcgtcgtggc cggcgtgctg agcggggccc acgatcggga cacgctgagc gcggccgggg
     8761 cggaccacgt catcgacacc gtggccgatc tgcccgacct gctggcgaac ctgtagccca
     8821 cgggtcacca cacgaccgcc ccgctacaag ttcggcaggc ggcaccgaac ttgtagccca
     8881 caggtcacca cacgaccgcc cagctacaag tccggcaggg cgcaccgaac tcgtagccca
     8941 cgggtcacca cacgaccgcc cagctacaag ttcggcaggc ggcaccgaac tggtagccca
     9001 caggtcacca cacgaccgcc cagctacaac atcggcaggt ggatcgggtg gtcactcctc
     9061 ggcgaacagg gcgtcgatga actcgtcggc gtcgaagtcg accaggtcgg cggcggtctc
     9121 gccgagaccg acgagcttga tcgggatgcc gagctcggaa cggatggcga agacgatccc
     9181 gcccttggcg gagccgtcca tcttggtcag caccacaccg gtcacgtcgg tcgcctcggt
     9241 gaactgacgg gcctgctgca gaccgttctg gccggtcgtg gcgtcgagca cgagcaggac
     9301 ctcggtgacg gtgccgtcgc ccttgtcggc gacccggcgg accttggcca gctcgtccat
     9361 caggttcgac ttggtgtgca accggccggc ggtgtcggcc agcacgacgt cagccccctt
     9421 ggccgatgcc gaggcgatgg cgtcgaagat gatcgagctc ggatcgccac cctcggcgcc
     9481 tcggacgatg tcggccccgc agcggtcggc ccacatggtg agctgctcgg cggccgcggc
     9541 ccggaaggtg tcaccggcgg ccatgaccac cgtccggtcc tggtcggcca accgacgacc
     9601 cagcttgccg atggtcgtcg tcttgccgac gccgttgacc ccgacgaaca accacaccgt
     9661 gggcgcggtc tcggccacat gcagctcgac cgaacccgcg agtcgggcct tcatctcgtc
     9721 cttgaccgcg tcgatcagct cgtcgggggt ggtgaggccg cgctccttga ccgtcgagcg
     9781 caccgcgtcc agcagctcct gggccgggcc gatgcccacg tcggcccgga tcagcgcctc
     9841 ttcgaggtcg tcccaggtct cctcgtcgat ggtcgaccgc gacatgatcg aaccgacgta
     9901 gcccgagaag gccgatcggg cccgcgacag ccggtcgcgg aacgacggcc ggacgagctc
     9961 ctcgacaccg ccagcctgtt cggactccca ctcggcctcg gccgcctcgg ccgccgcgat
    10021 gttggccgct tcttcctctt cggtgagctc cgggccggtg atctcgaccg tgccaccgaa
    10081 catggcgccc cggggttcga cgacctcgac ctcgggttcc tcgagcgtgt cgcggtccga
    10141 cggcgtcggg gcctcgcggg gcgccacggc ccccggcccg gcctcgtcga tctgctcgag
    10201 atccacctcg ggcggcggtt cgagcgtctc cccgcgcccc cgtcgcgaca cgagcgttgc
    10261 gcccgccacg accgccagga cggcgacgag ggccaggatg acggcgagga tcacgagtgg
    10321 ttccatcggc ggggagccta caagtgcgct ggagcgggca agctgaagcg gggatcagct
    10381 tccccgaggt cgcgacgcgc caccgtccgg ggagcccgta ccgtgacgtg accgacacga
    10441 ccaccgaaca cgacccaccg accccaccga ccccgccgcc cgacagcccg tcgccggcga
    10501 accgccgaag agagagcgac gaccgtggac cagccttccc tgacgacccc cacccccgct
    10561 gcggagatcg agcgcctgct gttcgagctc aagaaggtcg tcgtcggcca ggaccgggcc
    10621 atcgagcggg tcctggtctg cctgatcgcg ggtgggcact gcctgttgga gtcggtgccg
    10681 ggcctggcca agaccctcat ggtcgagacg ttggcctcgt cggtgggcgg cagcttctcg
    10741 cgcatccagt tcacccccga cctgctgccc gccgacctgg tcgggacacg catctaccgg
    10801 gcctcgtcgg agcagttcga cgtggagctc gggccggtgt tcgccaactt cgtgctcgcc
    10861 gatgagatca accgggcccc ggcaaaggtc cagtccgctc tcctcgaggt gatggccgag
    10921 caccacgtga cgatcggcgg acagcgcttc gacgccccgg agccgttcct ggtgctggcc
    10981 acccagaacc cgatcgagtc cgaaggcgtc taccccctcc ccgaggccca gcgcgaccgc
    11041 ttcctgatga aggtgctcct cgactacccc gcacccgccg aggaggtgga gatcgtccag
    11101 cggatgggca ccaacccgcc ccacgccgaa caggcgctca ccctggccca ggtcgagggg
    11161 ctccaggccg ccgccgagga ggtcttcgtg gaccgcagcg tgctcgagta cgccgtcaac
    11221 ctggtgctgt gcacgcggac gccggagaac ttcggcctgc ccgagctgcg cacgttcatc
    11281 gagctcgggg ccagcccccg cgcctcgctc ggcctcatcc gcggcggtcg tgcgctcgcc
    11341 ctgctgcggg gccgcaacta cctcaccccc caggacgtgt tcgacgtcgc ccccgagatc
    11401 atgcgccacc ggatgctgct gtcctacgag gcgctcgccc aggacgtcga cgtcgagcag
    11461 gtcctggccc gcgtgctggg caccgtgccg gcccctcggc tgagcccgac gcagggcggc
    11521 ggcgacgcct actacggaac gtccaccgac gccggggcgt ggaacgctcc ggccgccagc
    11581 ggcgccgctg cggcctgggg gaacggcgct tcgtgagcgg cctctcgcac accctcggtc
    11641 tcgtcgggga ccgcccggcc gaggagatcc tccgccggct gaccctcgac gtgaaccgca
    11701 agctcgacgg gatgctccac ggcgacttcc tgggcctggt ccccggccgg ggcaccgaac
    11761 ccggcgagac ccgcgcctac agccccggcg acgacgtccg ccgcatcgac tggaacgtca
    11821 ccgcccggat gcaggacccc tacatccgcg agacgatcgc tgatcgtgag ctcgaggcgt
    11881 ggctggtcgt cgaccgctcg gcccgcctcg acttcggaac cgcgacctgc gagaagcggg
    11941 acctggcact ggccgccgcg gccggtgtcg ggctcctcac cggccggggc ggcaaccggg
    12001 tcggggcgtt gatcctgcgc ggctcggagc tgacgacggt gcctccccgc aacggtcgga
    12061 cccacctgct cgggatcctc gagcgaatca tgtccacccc ccgcggagac ggcagcggta
    12121 cgagcgacct ggccgcgacg ctgaagcggg ccggggccat cgccccccgc cgcggactgg
    12181 tcgtcgtcat ctcggacttc ctcgacgacc ccgaccgctg gcgcaccgcc ctgggcacgg
    12241 tggcgctgcg ccacgcggtg ctgtgcgtcg aggtggtcga tccccgcgag ctggagctcc
    12301 cgccggtcgg gttggtgcag ttccgcgacc cggcaaccgg cgccacccgc gaggtcaaca
    12361 ccaacgactc cggcacccgg gcccgctacg ccgaggctgc ggcacgccag cgcgccacga
    12421 tcgccggcgc catccgcggc gccggagccg accatctgca gctgcgaacc gaccgggact
    12481 ggatcctcga cctggcccgg tacgtgtccc gacgccgccg acgggccgag ctggccgcca
    12541 ccgcagcacc ccgctgagag aactcccgct gtgagagatg accgacaccg aacgaccgac
    12601 gacgaggccg acaacagatg actgactggt tcctgcaccc cgagcgcctg tggctgatct
    12661 tggtcgtcgt cggcctcgcg gctctgtacg tcggcctgca gttcacgagc ccccgctacg
    12721 cggtccgctt ctccaacctc gagctgctcg actcggtcgc cccccgccgc ccgggctggc
    12781 ggcgccacgt cgtggccgga tgcttcctgg tcgcggcggc agtcctggtg gccgcgttcg
    12841 cccagccggt catgaccgag aaggtgccct cggagcgatc gacgatcgtc ctggccatcg
    12901 acacctcgct gtcgatggcg gccaccgacg tgtctccgaa ccggatcgaa gccgcccagg
    12961 ccgcggccaa ggagttcatc gacaagctgc cggaccagct caacgtcggc ctgatcagct
    13021 tcgcgggcag cgcccagctg ctcgtgcccc ccacccagga ccacgccaag gtgaacgcgg
    13081 cgatcgacgg cctcgagctc gacaagtcga ccgcgatcgg tgacgccacc aagctcgcgg
    13141 tgcaggtgat cgaggaccag gcccgtggca ccgacggcaa gaaggtcgac ggcgcggtcg
    13201 tggtcatgtc cgacggcgag acgaccgtcg gactgccgac agccgacgcg atcccgatcg
    13261 ccaagaaggc cggcgtggcc atcacgacca tcgcctacgg cacccaggag ggcgagatca
    13321 ccgtcgacac cgacggcgac ggcgtcggcc aacgcacccg ggtgccggtc aacgtcgagg
    13381 agctgcggag cctggccgag gggaccggtg gcaccgccta caccgccgag accgcccaga
    13441 gcctgcgctc ggtgtactcc gagctgggct cgaccatcgg ctacgacgag aagtcccagg
    13501 acgtcacgta ccgcttcgtc ggcctgagcc tggtgctgct gctggtcgcg gcaggattct
    13561 cgcttcgctg gttcagccgc ctgccctgat ccggcgaccg ccgacacgcc ccgaccgcgg
    13621 ccacggtctc gatcgaggac ggtctcaatc gaggacggtg gcggcccgct cggacacgac
    13681 ccgggacgag cccgccggtt ccatcgtgac gccgtagagg acgtccgccg cctccatggt
    13741 gcgcttctgg tggctcacca cgatcagctg cgcagcgtcg cggaactcgg cgatcaggtc
    13801 gaggaaccgg tggaggttca cgtcgtcgag cgcggcctcc acctcgtcca tcacgtagaa
    13861 cggcgacggc cgggagcgga acaccgcgaa caggtacgcc agagcggtca gcgagcgctc
    13921 gccgcccgag agcagcgaca gcttgcggac gttcttgccc gacggccgtg cctcgagctc
    13981 gatgccggtc tcgagcaggt tgtccggatc ggtgagccgg agccgaccct gaccaccggg
    14041 gaagagggtc tcgaacagca gggtgaagtt ctggctgacg tcggcgtagg cggcggagaa
    14101 gacgctgacg atctcctcgt cgaccgcctt gatcaccttc gagagctcgc gccgggtctg
    14161 acggatgtcc tcgagctggg actcgaggaa cgtgtgacgc tcggagagct cgtcgaactc
    14221 ctggagggcc agcgggttga tcgggcccat gaggcgcagc tcgcgctcga gctcccgcac
    14281 ccgggcgggg gcgctgacgc cctcgggcag cgacgggcac tcggcggcca tcgcggcgtc
    14341 gggctcggtg tcgagatcgt gacgaagtga ctcgaccgcc gtgtcgaggc gcatccggga
    14401 ctcggcatcg gagatctcgg ccttctggag ccgggcccgg gtctcttcca gcgaccgctc
    14461 cgaggtgctc cgctcggagc gcaggccgtc gagcaggttg gtgacctcgc gggtggcctc
    14521 ggactgacgg cggcgctcct gccggagctc ggccagcacg ggttcgatcc ggctcagccg
    14581 gtccgagagg aaggagtgga gtcggtcggt ggcgaccaac cgggcctcga gcacgatccg
    14641 acggtcggcc gcagcggcga cctcggcctg gttgcgcgcc agccgttcgc cgatctgctc
    14701 cagtcgggaa ccgacgtagc ggcgccgctc gtcgagcgcc gcgatccgca cctccagatc
    14761 ggtccgacgg gcctggacgt ccgcgccacg ggcgtcgagg cgctcacggg cctcggccat
    14821 ggcccgcgcc cgttccccca gctcggcctc ggcggcctcc agcgccggaa gctgcccgga
    14881 gagctcggcg acccggacct cttcgcgtgc caagcgatcg acgagctcct gcaggtgggc
    14941 acccagcgcc tccaactcgc ccgcggcctc ctgagcgtcg tggtccagtc gtgcggcctt
    15001 gtcggccagc gcggcacggt ccgcggcggc ccgatcccgt cgacgcgctg cctgctgctc
    15061 ggcgtcacgg gtggcggcca ccgactcgcg ggccgactcc accccgcgcc tagcccggtc
    15121 ccgctcggag ccggcctgtt cggctcggtc gcgtgcctcg tccagcgcag cgcccgtggc
    15181 gcccgagccg gcggcccgca gtcgccaacc cgccgccgcg aagcggtcgc cccgacgggt
    15241 gaccacgacg gccgacggcg acgacaacga tgccgacagc gcggcgtcga ggtcaccgtc
    15301 gacgaccacg gccccgtcga gaagggcatc gagcagtgcg tcgatcgccg cggacgccgc
    15361 tccggcaccg gcagcggacc gcacgtggcg gcggaccggc tcgccgacgc cgacggt
//
LOCUS       DAOQOD010000070        15107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1396_length_15107_cov_12.714058, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000070 DAOQOD010000000
VERSION     DAOQOD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 15107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15107
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_15107_cov_12.714058"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLZ72_08480"
     CDS             complement(<1..271)
                     /locus_tag="PLZ72_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36370.1"
                     /translation="MEQFVEGVGGEVFAAAVAATRISPDVWWRLRWVCLVWQHRNIGV
                     WSGCRAWRAGGSEAAGSVVEGELAAVSDDLPAFPVQFVVMEAAQQD"
     gene            complement(339..1160)
                     /locus_tag="PLZ72_08485"
     CDS             complement(339..1160)
                     /locus_tag="PLZ72_08485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA36371.1"
                     /translation="MGHDAITGGDPPPTGLAAPHDHGGPLWWDPLSHRPAHANPETGP
                     LLGVVGVLVGSNVIANEVLPSWAYIPWNIGVAGALVWVARRWGRTTPRELGLAPDRIR
                     SGLLWGGLAALAVVGTTAVGALLPVTRELFQDDRAAGLSLGGLATYALVEIPFGTVLA
                     EETMFRGVLPAMFRRRFAHRRNWAVRGDLAAATLFGLWHVLPSLDLAASNTALKDLPF
                     GLGVPVAIAGSVAATAVAGLGFTWLRNRSGSLVAPVLLHWAINGSGIVAAWIVQH"
     gene            complement(1172..3238)
                     /locus_tag="PLZ72_08490"
     CDS             complement(1172..3238)
                     /locus_tag="PLZ72_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HNA36372.1"
                     /translation="MTDSTTAGASAEPTSGDLPATGTADLEEVGVPAGSAAVSRLESV
                     LSLVSPVTSSPGAPLETTNFIRSVQPSLMPRTSLHQGVVSALSILTARKVSKVVERLT
                     DRVTFGTDSLTARLVARAALVAGGNALAAVPDPDDQRWPAGVSRLTGELLQFGAAGGA
                     LYDVLSKLDTGESDNVRSLRRLGITTGVSVGFAAYASRRFLTERRSFFTDDFDQLSLT
                     LPQTMLNTAAVTAVGTGLGASFKLTRRGWIRYFGPGITRNVLARGVNAGLWAGAATGL
                     YWAGISYIGRSNEKIDPGYSQAPTDPLVSGSPESLASFEELGQQGRRFVLDSVDPDLI
                     DETLDESGARTPIRIYIGFNDHPIHSSSRAETALAELERTGAYDRKYLLLVSPTGTGW
                     VDHTVQEATEFFARGDIATVCIQYGRYPSFLSLQKVRQGRRQFRTLALGVHQRLQGMA
                     PEDRPEVLVFGESLGAWSSSDVVMKSGIDGFDSYSISRALWFGMPHLARWSKAGLDRP
                     GAMTPPGTVGVFDRWEELERLTPAERDALRVVQLSHDNDPITHVDPSILFRPPDWLGE
                     PRGRNVPAGQRWFPVVTFLQTLIDAANAMRNEPGEFRSTGHDYRADTARFVAEGYRLP
                     YSEEQLAAVEVQLRRLEKERAARAKGTEDGDAPAGSDGAAAADGPVRDGRTAGPAWLR
                     KAGIGG"
     gene            complement(3293..3366)
                     /locus_tag="PLZ72_08495"
     tRNA            complement(3293..3366)
                     /locus_tag="PLZ72_08495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3330..3332),aa:Pro,seq:tgg)
     gene            3452..>4806
                     /gene="tig"
                     /locus_tag="PLZ72_08500"
     CDS             3452..>4806
                     /gene="tig"
                     /locus_tag="PLZ72_08500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNA36373.1"
                     /translation="MKATLEPVPDTPDLEPKVKLTVQVEEVELEPAIAAAWKEIAKEV
                     KIPGFRPGKAPRALMEKQIGSGYARAEALNRAIPEFYGEAIIENDVDVIAQPELDITD
                     GEESGDVTFTAVVGVRPTINIAGYGSLRVEVPSPEVATEDIDAQIDRIRSQYGELEAV
                     ERAAASDDFVTIDISGTQDGEEVDGLTADDYLYRVGSGMIASELDENLLGASAGDVVE
                     FTAEHPDPEQDDVDFRVVVKEVKERVLPELTDEWVAEATEFETVEQLTDDVRSRLAEG
                     AENRTRGAVRTGVVTELTQLVDEEIPESLVSAEMSSQLQNVIMRLQQQGIGLDDYLRV
                     IGQDQDDFLTGLKESAVDGVKADLALRAVVAAEQIVAPEDEVDHAIEHMIGDADISLD
                     DAREQLRRGGQLSELRSDLAAQKAIDWLVAHAEVVDPDGNAIDERFLQPLEHDHDHDD
                     H"
     assembly_gap    4807..4906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5010..5654
                     /locus_tag="PLZ72_08505"
     CDS             5010..5654
                     /locus_tag="PLZ72_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNA36374.1"
                     /translation="MPPTQPQSYLVPNVIEQTSRGERYSDLYSRLLRENIIFLGTPID
                     DAVANLICAQLLFLESENPDKDISIYINSPGGDINALMAIYDTMQYIRPEITTLCFGQ
                     AASAAAVLLAAGTPGKRLALPNARVLIHQPYAQSGYAQVSDLEIAANEIIRLKETLEQ
                     VLSRHTGQSVEKISKDTDRDYVMTAAEAKEYGIIDEVIEARDRVDNSGPIAAIR"
     gene            5744..7024
                     /gene="clpX"
                     /locus_tag="PLZ72_08510"
     CDS             5744..7024
                     /gene="clpX"
                     /locus_tag="PLZ72_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNA36375.1"
                     /translation="MAKFGDGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAETGDLVLEELPKPAEIFEFLDDFIVGQDRAKKILSVAVYNHYKRVQHGATR
                     SGPDEVELAKSNIMLIGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLIQAADFDVKKAETGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGT
                     TASVPPQGGRKHPHQEFIQIDTTNILFICGGAFAGLDKIVEQRVGKKGIGFGAEVRSV
                     TERREGDLFAQALPEDLLKFGLIPEFVGRLPVLGVVDNLDREALIQILVEPKNALVKQ
                     YRKFFELEEVDLEFTDAALAAVADQALARGTGARGLRAILEEVLLGVMYELPSRSDVS
                     KVVVDEDSVLEKVNPTLVPRSGKPRSSRPRRAAS"
     gene            7074..8387
                     /locus_tag="PLZ72_08515"
     CDS             7074..8387
                     /locus_tag="PLZ72_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNA36376.1"
                     /translation="MGIIESLAWLDSHLDHESSSAGIAAGQVDGLDLTAMSELMALLG
                     DPQLDVPTIHVTGTNGKGSVTAMIVGLLQAHGLSVGSATSPHLERINERLRRDGEPID
                     DQTLSEVLDGIRSVEPLLSVRPSWFELVTAASLRWFAEAPVGVAVVEVGLLGRYDATN
                     VVRSQVAVVTGVGGDHTDFAPGWELAVAGEKAGIIEPDAPAVLGRMTPELREVFRAEG
                     PASLVAEDVDFEVLASRPAVGGRLVDVAGVHDRYDEIFLPLHGSVQAHNAAVAVAAVE
                     SFFDRALEVDLVRDGLALVRLEGRVEVVSHQPLVVLDGAHNPDALNALGATLDEEFST
                     VGSRVVVLGLLAGRDPDAAVAAVRSLRPDLVVCTSADAGERALEPAVLAEACERAGLA
                     AQVEPSPTEAVRRAVGQAQEEDMVVVTGSFRLIGPARAAVAAALS"
     gene            complement(8496..8909)
                     /gene="ndk"
                     /locus_tag="PLZ72_08520"
     CDS             complement(8496..8909)
                     /gene="ndk"
                     /locus_tag="PLZ72_08520"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNA36377.1"
                     /translation="MTRTFIMCKPDAVERGLVGEIIARIERKGLRIVAMDLRVPDTAL
                     AQAHYEEHADKPFFGELVTFLTRGPVVAMVVEGPDDGTWQLMRTLIGKTKVEDAQPGS
                     IRGDFATTTNENLVHGSDGTESAAREIGLWFPNFA"
     gene            9178..10215
                     /locus_tag="PLZ72_08525"
     CDS             9178..10215
                     /locus_tag="PLZ72_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNA36378.1"
                     /translation="MARFNSSFLGRDMAVDLGTANTLVYVRGEGIVLCEPSVVAVQVK
                     DGRAVAVGTEAKRMIGKTPAHIQAIRPLKDGVISDFEVCETMLRYFIQQVHSSRFSKP
                     RMIICVPSGVTGVEQRAVQDAAEFAGARKPARIIEEPMAAAIGAGLPVHEPTGNMIVD
                     IGGGTTEVAMISLGGVVTSQSVRVGGDELDDAIIQYIKKEYSLALGERTAEVVKMELG
                     SAWELEQELQAEIRGRDLVTGLPKTIVTSTHEIREAIEEPVSSIVDAVKVTLDKTPPE
                     LAADVMERGIVLAGGGALLKGLPTRLAQETGMPIRIAPRPLEAVALGSGMALEEFPAL
                     EQAGLFSSHRD"
     gene            10221..11057
                     /gene="mreC"
                     /locus_tag="PLZ72_08530"
     CDS             10221..11057
                     /gene="mreC"
                     /locus_tag="PLZ72_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNA36379.1"
                     /translation="MRTVETRRRSRYVLLVLSLLAVTLITLDSRGVGVFDGIRNTAGD
                     VFAPVGDAFAWVTSPISNAWGGMSDYDDLKAENERLRQRVDELEGGEAEAANAVEQLE
                     RLQEQLQIEFVGDIPTQIARVATGPYSNFDTFRTEIDKGSDAGLAVGMPVVTRAGLVG
                     RLERVSRTRSVVQLATDPDFTIGVRLASTQDIGLGHGGGDSNRFVVDRGIDVGDPVEP
                     GEVVLTSGLDDSVMPKDIPIGLVDKVRPDENTGMQLLLVDYAVDFSQLDVVQVLKWTP
                     PR"
     gene            11080..11589
                     /gene="mreD"
                     /locus_tag="PLZ72_08535"
     CDS             11080..11589
                     /gene="mreD"
                     /locus_tag="PLZ72_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNA36380.1"
                     /translation="MTDGRSLIRWVLIVSVATVLQIGLATQVRVAGVHPDLLLLVSIC
                     AGLTSGPGTGAGIGFACGLVLDLFLPGRFGVTALAYLLVGYGSGLAGDAVARPTRAIS
                     VGLVAAASAAGVLLYAAIGQLLGQRSLSDPRLGAIVGIVTAFNAVLALPVLAVCRWAH
                     PDDTRLRLR"
     gene            11659..13782
                     /gene="mrdA"
                     /locus_tag="PLZ72_08540"
     CDS             11659..13782
                     /gene="mrdA"
                     /locus_tag="PLZ72_08540"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNA36381.1"
                     /translation="MDTESRQVRLSALGIVALSLFLALFVRLWFLQGIDRQKFEVASA
                     SNRLRVIHEEGPRGRILDRNGKVIVDNRTSRVVALDREPLRKMDDDERNAHFEELART
                     MTELGRPTKVALIQKRYDDKRYAPQEYVPIVEDVTEEVEIHLSERAERFPGVVVETRS
                     VRSYPYGSLAGQVLGYVGEINEKELVAKGGTLPAGVTTTDPPETTTTTPGVPPKPYQP
                     GDLIGKSGVERSYEDYLRAVPGNRTIEVNAKGDLIDVVKRETPRAGDDVWLSIDIDLQ
                     AEAERLLAAKIDSLRGRTDKTGARFDAPQGSVVIQEPTTGQVLAMASYPPFDPSSTVN
                     GISSELWAQLNDSANGQPLYNWALQGMYAPGSTFKPFTAFAAMSSGLLPTGNESYVDN
                     GVYRLDVCKGTKCEFRNAGGTPHGRVNVPKSLTVSSDVYYYWIGEKFWVGRDRFGDTP
                     IQDAAAKFGIGSPTGIRLPGENSGRLPTPAARKEAYEANPGKFATGDWRTGDNIITSI
                     GQGDVLVTPLQLVDAYATLANGGTRYRPQIVSKVTRIKDAALPPGQEGNFDVVTTVEP
                     EALGKVDMTPQQHGQIVSGLVGVTQSGEGTAAASWRASPTAWPMAGKTGTAQVRGKAD
                     TALFAGWGPTGPGAVPQYAISVVVPEAGFGGDVAAPLAFRILQPLSHGQLTPACTVVD
                     RSACDMAALAANQASQSDVGSGGPG"
     gene            13782..14903
                     /gene="rodA"
                     /locus_tag="PLZ72_08545"
     CDS             13782..14903
                     /gene="rodA"
                     /locus_tag="PLZ72_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNA36382.1"
                     /translation="MTRTLTPSGDRFRSTDLTAPWRHVDTVLLGVVVLVVVTGTAMVY
                     SATRNLAGGSGYMVRQGLFVVLGAALMVGISLIDYRRIADWWQVIYGLSLIGMAGIFT
                     PLGAVVNGTKGWYRFGPFSLQPAEFAKLGVILAVAAYLSQSDRVDLRRLAVALGLFGV
                     PTVMTLLQPDLGSALVFIVIALGMLVVGGVQMRHLLVLVALGLVAVLGILNSGTLDQY
                     QKDRLTSFVTPDGQSYNVSQAQQAIGSGGLTGYGFTKGPQTRGGYVPEQETDFIFTVA
                     GEELGFVGAGALVVLLGLMVWRVWRNAQLAGDTLGTLICVGIMSMMLFHIFENIGMTL
                     GIMPVTGIPLPFVSYGGSSMMTSFAAVGLVQSVHMHRYA"
BASE COUNT         2316 a         5327 c         5205 g         2159 t
ORIGIN      
        1 tgtcttgttg tgcagcttcc atcaccacga actgcaccgg aaacgctggc agatcatccg
       61 acacggcagc caactcacct tctacgaccg aacccgctgc ctcggatcct ccggcccgcc
      121 aggcccgaca cccggaccac acgccgatgt tgcggtgctg ccacaccaga cacacccacc
      181 ggagccgcca ccagacatcg gggctgattc gtgtcgctgc cacggcggcg gcgaacacct
      241 caccgcctac gccctcgacg aactgctcca ccacctcctc accgccgcct gaacccgaac
      301 ccgctgaaga tcaccacacc cgatcggtgg atccaggttc agtgctggac gatccaggcg
      361 gccacgatgc cgctgccgtt gatcgcccag tgcaggagca cgggtgcgac caggctgccc
      421 gagcggttcc tcaaccaggt gaagcccagt ccggccaccg cggtggccgc cacgctgccg
      481 gcgatcgcga cggggacacc caggccgaag ggcaggtcct tcagcgcggt gttgctcgcc
      541 gccaggtcga gtgacggcaa cacgtgccac agcccgaaca gggtcgcggc cgccaggtcg
      601 ccgcgcaccg cccagttccg acggtgggcg aagcgcctcc ggaacatcgc cggcaggacg
      661 ccgcggaaca tcgtctcctc cgccaggacc gtgccgaacg ggatctcgac cagggcgtag
      721 gtggcgagac ctcccagcga cagcccggca gcccggtcgt cctggaacag ctcgcgggtc
      781 actggcagca gggcgccgac ggctgtcgtt cccaccaccg ccaaggcggc caacccgccc
      841 cagaggagac cggagcggat ccggtccggt gccaggccga gctcccgggg agtcgtccgg
      901 ccccaccgtc gggcgaccca caccagggct cccgccacgc cgatgttcca ggggatgtac
      961 gcccaggacg gaaggacctc gttggcgatg acgttcgaac cgaccaggac gccgacgacg
     1021 cccaggagcg ggccggtctc gggattcgcg tgcgcgggcc ggtgcgacag cgggtcccac
     1081 cacagcggtc ccccgtgatc gtggggcgcg gcaagccccg tcggcggcgg gtcgcccccg
     1141 gtgatcgcgt cgtggcccac cccgtggccg ctcagccgcc gatgccggcc ttgcgcagcc
     1201 acgccggacc cgcggtgcgc ccgtcgcgga ccggaccgtc ggccgcggcg gcaccgtcgg
     1261 agcccgccgg cgcgtcgccg tcctcggttc ccttggcgcg cgccgcgcgc tccttctcga
     1321 gtcgacgcag ttgaacctcg accgcggcga gctgctcctc ggagtacggg agtcggtagc
     1381 cctcggcgac gaagcgggcg gtgtcggccc ggtagtcgtg gccggtggag cggaactcgc
     1441 ccggctcgtt gcgcatcgcg ttggcggcgt cgatcagcgt ctgcaggaac gtcaccaccg
     1501 ggaaccagcg ctgccccgcg ggcacgttgc ggccgcgggg ctcgcccaac cagtccggcg
     1561 gccggaacag gatcgacggg tcgacgtggg tgatcgggtc gttgtcgtgg ctgagctgga
     1621 ccacccgcag ggcgtcacgt tcggcggggg tgaggcgttc gagctcctcc cagcggtcga
     1681 acacgccgac ggttccgggc ggggtcatgg ccccggggcg gtccaggccg gccttggacc
     1741 agcgggccag gtggggcatg ccgaaccaca acgcccgact gatgctgtag gagtcgaagc
     1801 cgtcgatccc ggacttcatg accacgtccg acgacgacca ggcgcccagg ctctcgccga
     1861 agaccaggac ctcgggccgg tcctcggggg ccatgccctg gagtcgctgg tgcacgccga
     1921 gggccagggt gcggaactgg cgacgcccct gacgcacctt ctgcagcgac aggaacgacg
     1981 ggtagcggcc gtactggatg cacaccgtgg cgatgtcgcc ccgggcgaag aactcggtgg
     2041 cctcctgcac ggtgtggtcg acccagccgg tgccggtggg ggagaccagg agcaggtact
     2101 tccggtcgta ggcgccggtc cgctcgagct cggcgagcgc cgtctcggcc ctcgacgacg
     2161 agtggatcgg gtggtcgttg aagccgatgt agatgcggat gggggtccgg gccccggact
     2221 cgtcgagggt ctcgtcgatc agatccgggt ccaccgagtc gagcacgaac cgacggccct
     2281 gctgtccgag ctcctcgaag gacgccaggc tctcgggaga ccccgacacc aacggatcgg
     2341 tgggagcctg cgagtagccg gggtcgatct tctcgttgga gcggccgatg taggagatgc
     2401 cggcccagta gagacccgtg gccgctccgg cccacagccc ggcgttgacg ccccgggcca
     2461 ggacgttgcg ggtgatgccg ggtccgaagt agcggatcca cccgcggcgg gtcagcttga
     2521 acgatgcccc caggccggtg ccgacagcgg tgaccgccgc ggtgttcagc atggtctgcg
     2581 gaagcgtgag cgacagctga tcgaagtcgt cggtgaagaa cgaccgccgc tcggtgagga
     2641 agcggcgtga cgcgtacgcg gcgaagccga ccgacacccc ggtggtgatg ccgaggcggc
     2701 gcaggctccg cacgttgtcg gactcgccgg tgtccagctt ggacagcacg tcgtagaggg
     2761 cgccgccggc cgctccgaac tgcagcagct cgccggtgag gcgggagacc ccggcgggcc
     2821 agcgctggtc gtcggggtcc ggcacggcgg ccagtgcgtt gccgccggcg accagagcgg
     2881 cgcgggccac caggcgggcg gtcagcgagt cggtgccgaa ggtcacccgg tcggtcagcc
     2941 gctcgacgac cttgctgacc ttgcgggcgg tcaggatcga cagcgcgctg accacgccct
     3001 ggtgcagcga ggtgcgcggc atcagcgacg gctgcacgga gcggatgaag ttcgtggtct
     3061 cgagcggtgc gccgggcgac gacgtcaccg gtgacaccag cgacagcacc gactcgagtc
     3121 gggacacggc cgcggatccg gcgggcacgc cgacctcctc gagatcggcg gtcccggtgg
     3181 cggggaggtc gcccgaggtg ggctccgcgg acgctccggc ggtggtgctg tcggtcatgg
     3241 ccatgagcct acggggagcg tcgtacgctt cggctcaccc gatcggagtg agtcgggacg
     3301 gcgagattcg aactcgcggc ccccggctcc caaagccggt gcgctaccag gctgcgccac
     3361 gtcccgtggg cgccgacact acccggaccg ccggatcgtc ggtgccggcg atgggaaccg
     3421 tcgatcggcg ccgttgtagg ctctcgcccc tgtgaaagcc acgctcgaac ccgttcccga
     3481 cacccccgac ctggaaccca aggtcaagct caccgtccag gtggaggagg tcgagctcga
     3541 gccggccatc gccgcggcct ggaaggagat cgccaaagag gtgaagatcc ccgggttccg
     3601 ccccggcaag gccccccgcg ccctgatgga gaagcagatc ggatccggct acgcccgggc
     3661 cgaggcgctc aaccgggcca tccccgagtt ctacggcgag gcgatcatcg agaacgacgt
     3721 ggacgtcatc gcccagcccg agctcgacat caccgacggc gaggagtccg gcgacgtcac
     3781 cttcaccgca gtcgtcggcg tccgtccgac catcaacatc gccggctacg gcagcctgcg
     3841 ggtcgaggtc cccagccccg aggtggccac cgaggacatc gacgcacaga tcgaccggat
     3901 ccgcagccag tacggcgagc tcgaggcggt cgagcgcgcc gcggccagcg acgacttcgt
     3961 caccatcgac atctccggca cccaggacgg cgaagaggtc gacggcctca ccgcggacga
     4021 ctacctgtac cgggtcggct cggggatgat cgcctcggag ctcgacgaga acctcctcgg
     4081 cgcctcggcc ggcgatgtgg tcgagttcac cgccgagcac cccgaccccg aacaggacga
     4141 cgtcgacttc cgcgtcgtgg tcaaggaggt caaggagcgg gtgctgcccg agctgaccga
     4201 cgagtgggtc gccgaggcaa ccgagttcga gaccgtggag cagctcaccg acgacgtgcg
     4261 ctcacgcctg gccgagggcg ccgagaaccg cacccggggg gcggtgcgga ccggggtcgt
     4321 caccgagttg acccagctcg tcgacgaaga gatccccgag tcgctggtct ccgcagagat
     4381 gtcgtctcag ctgcagaacg tcatcatgcg actgcagcag cagggcatcg gcctggacga
     4441 ctacctgcgg gtgatcggcc aggaccagga cgatttcctt accgggctga aggagagcgc
     4501 cgtcgacggc gtcaaggccg acctcgcgct gcgggccgtc gtggccgccg agcagatcgt
     4561 cgcacccgag gacgaggtcg accacgccat cgagcacatg atcggcgacg ccgacatctc
     4621 gctcgacgac gcccgtgagc agctgcgccg gggcggtcag ctctccgagc tgcgctcgga
     4681 cctggcagcg cagaaggcga tcgactggct ggtcgcgcac gccgaggtgg tggatcctga
     4741 tggcaacgcc atcgacgagc ggttcctgca gccgctcgag cacgaccatg accacgacga
     4801 ccacgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat cacgatcacg
     4921 actgatcgcc gggcgggcgc ggtagcgtgg atccccaccc ggacgggtcg accagcgtcg
     4981 gccccgcagc accaccagga gccgatcgag tgccccccac ccagccgcag agctacctcg
     5041 tccccaacgt catcgagcag acgagccggg gtgagcgcta ctcggacctg tactcgcgcc
     5101 tgctgcgcga gaacatcatc ttcctcggca ccccgatcga cgacgccgtc gccaatctga
     5161 tctgcgcgca gctgctgttc ctcgagtcgg agaaccccga caaggacatc agcatctaca
     5221 tcaactcgcc gggcggcgac atcaacgcgc tgatggccat ctacgacacc atgcagtaca
     5281 tccggcccga gatcacgacc ctgtgcttcg gacaggccgc ctcggcggcc gcggtgctgc
     5341 tggccgcggg gaccccgggc aagcgcctgg ccctgcccaa cgcccgggtg ctgatccacc
     5401 agccctacgc gcagtccggc tacgcgcagg tgtcggacct ggagatcgcc gccaacgaga
     5461 tcatccggct caaggagacc ctcgagcagg tgctcagccg ccacaccggt cagtcggtgg
     5521 agaagatctc caaggacacc gaccgcgact acgtcatgac cgcggccgag gccaaggagt
     5581 acggcatcat cgacgaggtc atcgaagccc gtgaccgggt cgacaacagc ggtcccatcg
     5641 cggcgatccg ctgacggatc gggcacaatg gttccccacg acgggccgct tcccgtcgtg
     5701 accacaggtt cgagcacgct cgacgcaccc cggaaggagc ccggtggcca agttcggtga
     5761 cggaggagag ctcctcaagt gctcgttctg cggcaagtcg cagaagcagg tcaagaagct
     5821 catcgcgggc cccggcgtct acatctgcga cgagtgcatc gacctgtgca acgagatcat
     5881 cgaggaggag ctcgccgaga ccggcgacct ggtcctcgag gagctgccca agccggcgga
     5941 gatcttcgag ttcctcgacg atttcatcgt gggtcaggac cgggccaaga agatcctgtc
     6001 ggtcgcggtc tacaaccact acaagcgggt gcagcacggc gccacccgct ccggccccga
     6061 cgaggtcgag ctggccaaga gcaacatcat gctcatcggg ccgaccgggt gcggcaagac
     6121 gctgctggct cagacgctgg cccgcatgct caacgtgccg ttcgccatcg ctgacgccac
     6181 cgctctcacc gaggccggct acgtcggcga ggacgtcgag aacatcctgc tcaagctgat
     6241 ccaggctgcg gacttcgacg tcaagaaggc cgagaccggc atcatctaca tcgacgagat
     6301 cgacaaggtc gcccgcaagt ccgagaaccc gtcgatcacc cgtgacgtct ccggtgaggg
     6361 cgtccagcag gcgctgctca agatcctcga gggcaccacc gcctcggtgc cgccgcaggg
     6421 cgggcgcaag cacccccacc aggagttcat ccagatcgac acgacgaaca tcctgttcat
     6481 ctgcggcggt gcgttcgccg ggctggacaa gatcgtcgag cagcgggtgg gcaagaaggg
     6541 catcggcttc ggtgccgagg tgcgctccgt caccgagcgt cgcgagggcg acctgttcgc
     6601 ccaggccctg cccgaggacc tgctcaagtt cgggctcatc cccgagttcg tcggacggct
     6661 ccccgtcctc ggcgtggtcg acaacctcga ccgcgaggcc ctgatccaga tcctggtcga
     6721 acccaagaac gcgctggtca agcagtaccg caagttcttc gagctcgaag aggtcgacct
     6781 ggagttcacc gacgccgcgc tggccgcggt ggccgatcag gccctggcgc ggggcaccgg
     6841 cgcccgtggc ctgcgggcca tcctcgagga ggtgctcctc ggcgtcatgt acgagctgcc
     6901 gtcgcgcagc gacgtcagca aggtcgtcgt cgacgaggac tccgtgctcg agaaggtcaa
     6961 cccgaccctg gttccccgtt cgggcaagcc tcggtcgagc cggccgcgcc gcgccgcgtc
     7021 ctgagccgac tccctcgtcc ggccgttcag ccgttccccg atcgacctga cccgtgggca
     7081 tcatcgaatc gctggcgtgg ctcgactccc acctggacca cgagtccagc tcggcgggca
     7141 tcgccgccgg tcaggtcgac ggcctcgacc tgaccgccat gagcgagctg atggcgctgc
     7201 tcggcgaccc gcagctcgac gtgccgacca tccacgtcac cggcaccaac ggcaaagggt
     7261 cggtgacggc gatgatcgtg gggctgctcc aggctcacgg cctgagcgtc ggcagcgcca
     7321 ccagccccca tctggagcgg atcaacgaac gcctccgtcg tgacggggag ccgatcgacg
     7381 accagacgct gtcggaggtg ctcgacggga tccgctcggt cgagccgctc ctgtcggtgc
     7441 ggccctcctg gttcgagctg gtgaccgccg cgtcgctgcg ctggttcgcc gaggcgccgg
     7501 tcggcgtcgc tgtcgtcgag gtcggtctgc tgggccgcta cgacgcgacc aacgtggtcc
     7561 gttcccaggt ggcggtcgtg accggcgtcg gcggcgacca cacggacttc gcccccggct
     7621 gggagctggc cgtcgcgggc gagaaggccg ggatcatcga gcccgacgcc ccggcggtgc
     7681 tgggtcggat gacgccggag ctgcgcgagg tgttccgcgc cgagggcccc gcgtcgctgg
     7741 tggccgagga cgtcgacttc gaggtgctcg ccagtcggcc cgcggtgggt ggtcgcctgg
     7801 tcgacgtcgc cggggtgcac gatcgctacg acgagatctt cctgccgttg cacggctcgg
     7861 tgcaggccca caacgccgcg gtcgccgtcg cggcggtcga gtcgttcttc gaccgggcgc
     7921 tcgaggtcga cctcgtccgt gacggcctgg ccctggtccg cctggagggc cgggtcgagg
     7981 tggtgtcgca ccagccgttg gtcgtgctcg acggagccca caaccccgac gcgttgaacg
     8041 cgctcggagc caccctcgac gaggagttct ccaccgtcgg ttctcgggtg gtcgtgctcg
     8101 gcctgctcgc cggtcgtgat cccgacgccg ctgtcgccgc ggtgcgctcg ctgcggcccg
     8161 acctggtcgt gtgcacgtcg gcggacgcgg gggagcgggc gctggagccc gcagttctgg
     8221 ccgaggcgtg cgagcgggcc ggtctggccg cgcaggtcga gccgtcgccg accgaggcgg
     8281 tgcgccgggc ggtcggtcag gcccaggaag aggacatggt ggtcgtcacc gggagcttcc
     8341 gcttgatcgg ccccgcccgc gccgctgtcg ccgccgcgct ctcctgaccg ccgcgcgcct
     8401 gaccgccgcg ctcctgaccc cagcgcgcag ctttgtcccg cacagacgcg gtgatcaccg
     8461 cgtctgtgcg ggacaaagcc tggtcttgag gcgcgtcagg cgaagttcgg gaaccagagg
     8521 ccgatctcgc gggccgcgga ctcggtgccg tcggagccgt ggaccaggtt ctcgttggtc
     8581 gtggtggcga agtcgccgcg gatcgagccg ggctgggcgt cctcgacctt ggtcttgccg
     8641 atcagggtcc gcatcaactg ccaggtgccg tcgtcgggtc cctcgaccac catcgccacc
     8701 acggggccgc gggtgaggaa cgtgaccaac tccccgaaga acggcttgtc ggcgtgctcc
     8761 tcgtagtggg cctgggccag agcggtgtcg gggacccgca ggtccatggc gacgatccgc
     8821 agccccttgc gctcgatgcg ggcgatgatc tcgccgacca gcccccgctc gacggcgtcg
     8881 ggcttgcaca tgatgaaggt cctggtcacg gcgggtgcgc tcctggggaa tcgtgtcggg
     8941 ggtgggtccg gaggccgacg agggcgatct ccggaggccg aaggctagga gcgcgaccgg
     9001 cacgcgggcg aacccggctg tgggcctgca ccgcgcagcg acatgggcca ctggtcgaaa
     9061 cggctcggac tcggtagggt cattgcagtt ctgttgcgga gctgagtccc gtggagtcgc
     9121 cccctccaca gcactctccg cgtcaacccg ttccctccac gtagtctccg aggaccaatg
     9181 gcacggttca actcgtcgtt cctgggccgc gacatggcgg tcgatctggg caccgccaac
     9241 acgctcgtct acgtgcgcgg agagggcatc gtcctgtgcg agccctcggt ggtcgccgtg
     9301 caggtcaagg acggccgcgc cgtcgcggtc ggcaccgagg ccaagcggat gatcggcaag
     9361 accccggcgc acatccaggc catccgtccg ctcaaggacg gcgtgatctc ggacttcgag
     9421 gtctgcgaga ccatgctgcg ctacttcatc cagcaggtgc acagcagccg gttctccaag
     9481 ccccgcatga tcatctgcgt gccctcgggc gtcaccggcg tcgagcagcg cgccgtgcag
     9541 gacgctgcgg agttcgccgg ggcccgcaag ccggcccgga tcatcgagga gccgatggcc
     9601 gccgccatcg gtgccggact gccggtgcac gagccgaccg ggaacatgat cgtcgacatc
     9661 ggaggtggca ccaccgaggt cgccatgatc tcgctcggcg gcgtggtcac cagccagtcg
     9721 gtgcgcgtcg gcggcgacga gctcgacgac gcgatcatcc agtacatcaa gaaggagtac
     9781 agcctggcgc tcggcgaacg caccgccgag gtcgtcaaga tggagctggg atccgcctgg
     9841 gagctcgagc aggagctcca ggccgagatc cgtggccgcg acctggtcac cggcctgccc
     9901 aagacgatcg tcacctcgac ccacgagatc cgcgaggcga tcgaggagcc ggtctcctcc
     9961 atcgtcgacg cggtgaaggt cacgctcgac aagaccccgc ccgagctggc cgcagacgtc
    10021 atggagcgcg gcatcgtgct cgccgggggc ggtgccctgc tcaagggcct ccccacccga
    10081 ctggcccagg agacgggcat gccgatccgc atcgcccccc ggccgctcga ggccgtcgcg
    10141 ctcggctcgg gcatggcgct cgaggagttc ccggcgctgg agcaggccgg cttgttctcc
    10201 tcgcaccgcg actgaccccg gtgcgcaccg tcgagacgcg acgtcgctcg cggtacgtgc
    10261 tgctcgtcct cagcctgctg gcggtcaccc tgatcacgct cgactcccga ggcgtcgggg
    10321 tgttcgacgg catccgcaac accgccggtg acgtgttcgc cccggtggga gacgcgttcg
    10381 cgtgggtgac cagcccgatc agcaacgcct ggggcgggat gagcgactac gacgacctga
    10441 aggccgagaa cgagcggttg cgtcaacggg tcgacgagct cgagggggga gaggccgagg
    10501 ccgccaacgc ggtcgagcag ctcgagcggc tccaggagca gctgcagatc gagttcgtcg
    10561 gagacatccc gacccagatc gcccgcgtcg ccaccgggcc gtactcgaac ttcgacacct
    10621 tccgcaccga gatcgacaaa gggtccgacg ccgggctggc cgtcggcatg ccggtcgtga
    10681 cccgcgccgg actcgtcggc cggctggagc gggtcagccg cacccggtcg gtggtgcagc
    10741 tcgccaccga ccccgacttc accatcggcg tgcgcctggc cagcacccag gacatcggcc
    10801 tgggtcacgg cggcggggac tcgaaccgct tcgtggtcga ccggggcatc gacgtcggcg
    10861 acccggtcga acccggcgag gtggtgctga ccagcggact cgacgactcg gtcatgccca
    10921 aggacatccc gatcggcctg gtcgacaagg tccgacccga cgagaacacc ggcatgcagc
    10981 tgctgctggt cgactacgcg gttgacttct cccagctcga cgtcgtccag gtgctcaagt
    11041 ggacgccgcc gcggtgaccg gccccgcggc ccgacggcca tgaccgacgg gcgatccctc
    11101 atccgctggg tgctgatcgt gtcggtcgcc acggtcctgc agatcggcct ggccacccag
    11161 gtccgcgtcg ccggggtcca ccccgacctg ctcctgctcg tgtcgatctg cgctggactc
    11221 acctcgggcc cgggaaccgg cgccgggatc gggttcgcat gcgggctggt cctcgacctg
    11281 ttcctcccgg gccgcttcgg tgtcaccgcg ctcgcgtacc tcctcgtcgg ctacggctcc
    11341 gggctggccg gcgacgcggt ggctcggccc acccgggcga tctcggtcgg gctcgtcgcg
    11401 gcggccagcg cggccggggt cctgctctac gccgccatcg gccagttgct gggacaacgc
    11461 tcattgtccg acccgcggct cggcgccatc gtcggtatcg tcacggcgtt caacgcggtg
    11521 ctcgcgctcc cggtgctggc ggtgtgccgg tgggcgcacc cggacgacac ccgactccgc
    11581 ctccgttgag cgcacgccgc ccgatcaccc gttccccatg tcgcaccgat ccccgagccc
    11641 gctgcgggcc cgctcctgat ggacaccgag tcccgacagg tccgcctcag cgccttgggc
    11701 atcgtggcgt tgtcgctgtt cctcgccctg ttcgtccggc tctggttcct gcagggcatc
    11761 gaccgacaga agttcgaggt ggcctccgcc tccaaccgtc tccgcgtcat ccacgaggag
    11821 ggtccccggg gtcggatcct ggaccgcaac ggcaaggtca tcgtcgacaa ccggacctcg
    11881 cgggtggtcg ccctggaccg cgagccgctg cgcaagatgg acgacgacga gcgcaacgcc
    11941 cacttcgagg agctggcccg gaccatgacg gagctgggta ggcccaccaa ggtggcgctg
    12001 atccagaagc gctacgacga caagcgctac gccccccagg agtacgtccc gatcgtcgag
    12061 gacgtgaccg aggaggtcga gatccacctg tcggagcgcg ccgagcggtt cccgggcgtg
    12121 gtggtggaga cccgatcggt gcgctcctac ccctacggat cgctggccgg gcaggtcctc
    12181 ggctacgtcg gcgagatcaa cgagaaggag ctcgtggcca agggtgggac cctgcccgcc
    12241 ggggtgacca cgacggatcc gcccgagacg accacgacca ccccgggggt tccgcccaag
    12301 ccgtaccagc ccggagacct gatcggtaag tctggggtgg agcgctccta cgaggactac
    12361 ctgcgcgccg tgcccggcaa ccgcaccatc gaggtcaacg ccaagggcga cctcatcgac
    12421 gtggtcaagc gggagacgcc ccgcgccggc gacgacgtct ggttgagcat cgacatcgac
    12481 ctccaggccg aggccgagcg cctgctcgcc gccaagatcg actcgctgcg gggccgcacc
    12541 gacaagaccg gagcgcgctt cgacgcaccg caggggtcgg tggtcatcca ggagccgacc
    12601 acgggtcagg tcctggccat ggccagctac ccgccgttcg acccgtcgag caccgtcaac
    12661 gggatctcgt ccgagctgtg ggcgcagctc aacgacagcg ccaacggcca gccgctgtac
    12721 aactgggcgc tgcagggcat gtacgccccc ggatcgacgt tcaagccgtt cacggcgttc
    12781 gcggcgatgt cgagcgggct gctgccgacc ggcaacgagt cctacgtcga caacggcgtg
    12841 tacagactcg acgtctgcaa gggcaccaag tgcgagttcc gcaacgcggg tggcaccccg
    12901 cacggacggg tcaacgtgcc gaagtcgctg acggtgtcct ccgacgtcta ctactactgg
    12961 atcggcgaga agttctgggt gggccgcgac cgcttcggcg acaccccgat ccaggacgcg
    13021 gcggccaagt tcggcatcgg gtcgccgacc gggatccgcc tgcccggcga gaactccgga
    13081 cggctcccca cccccgcggc gcgcaaggag gcctacgagg ccaaccccgg caagttcgcc
    13141 accggcgact ggcgcaccgg cgacaacatc atcacctcga tcggccaggg cgacgtgctc
    13201 gtcacgccgc tgcagctggt cgatgcgtac gccaccctcg ccaacggcgg cacccgctac
    13261 cggccccaga tcgtctccaa ggtgacccgt atcaaggacg cggcgctgcc gccgggtcag
    13321 gagggcaact tcgacgtggt gaccacggtg gagcccgagg cgctcggcaa ggtcgacatg
    13381 accccacagc agcacggtca gatcgtcagc gggcttgtcg gcgtgaccca gagcggcgag
    13441 ggcaccgcag cggcgtcgtg gcgggcgagc cccaccgcgt ggccgatggc gggcaagacc
    13501 ggcaccgccc aggtgagggg caaggccgac accgccctgt tcgcggggtg gggcccgacc
    13561 ggacccggcg cggtgcctca gtacgccatc agcgtggtgg tgcccgaggc gggcttcggt
    13621 ggcgacgtgg ccgccccgct cgcgttccgg atcctccagc cgctcagcca cggccagctg
    13681 accccggcgt gcaccgtggt ggaccgctcc gcctgcgaca tggcggcgct cgccgcgaac
    13741 caggccagcc agtccgacgt gggatccgga ggacccggct gatgacccgc accctcaccc
    13801 cgtcgggcga ccggttccgc tccaccgacc tgaccgcgcc ctggcgtcac gtcgacaccg
    13861 tgctgctcgg cgtggtggtg ctggtcgtgg tcacggggac cgcgatggtc tacagcgcca
    13921 cgcgcaacct cgccggcggc tccgggtaca tggtccgcca gggcctgttc gtcgtgctgg
    13981 gcgcggcgct catggtgggc atctcgctga tcgactaccg ccgcatcgcg gactggtggc
    14041 aggtgatcta cgggctgtcg ctgatcggca tggccgggat cttcaccccg ctgggcgcgg
    14101 tggtgaacgg caccaagggg tggtaccgct tcgggccgtt ctcgctgcag cccgccgagt
    14161 tcgccaagct cggggtgatc ctggcggtgg ccgcctacct gagccagagc gaccgggtcg
    14221 acctgcggcg cctggccgtc gccctcggct tgttcggcgt gccgaccgtc atgacgttgc
    14281 tgcagccgga cctggggtcc gcgctggtgt tcatcgtgat cgccctgggc atgctcgtcg
    14341 tcggcggcgt gcagatgcgt cacctgctgg tgctggtcgc cctcggactg gtggcggtgc
    14401 tcggcatctt gaactccggc accctcgacc agtaccagaa ggaccgcctc acgtcgttcg
    14461 tgacccccga cggccagtcc tacaacgtca gccaggccca gcaggcgatc ggctcgggcg
    14521 ggctgaccgg ctacgggttc accaagggcc cccagacccg gggcggctac gtgcccgagc
    14581 aggagaccga cttcatcttc accgtggccg gggaggagct cggcttcgtc ggtgccggcg
    14641 cgctggtggt cctgctgggt ctgatggtgt ggcgggtgtg gcgcaacgcc cagctggccg
    14701 gcgacacgct cgggaccctg atctgcgtcg gcatcatgtc gatgatgttg ttccacatct
    14761 tcgagaacat cggcatgacg ctcgggatca tgccggtgac gggcatcccg ctgccgttcg
    14821 tctcctacgg cgggtcgtcg atgatgacct cgttcgccgc ggtgggcctg gtgcagtcgg
    14881 tccacatgca ccgctacgcc tgacggcccg gggagcggtt ccgtcccccg gtcccgccgt
    14941 cggtacactt ggcccaccat gaccgcccac ctggccgacc cccgaggggg gcctcctgac
    15001 gcgggtggct ccctgtggga ccgcctggaa ccgctgctgc accgggtcca gaagccggcc
    15061 cgctacatcg gctgcgagga cggcgccgcc acccccgact ggtccgc
//
LOCUS       DAOQOD010000071        14891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1422_length_14891_cov_12.222499, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000071 DAOQOD010000000
VERSION     DAOQOD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_14891_cov_12.222499"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..311)
                     /locus_tag="PLZ72_08550"
     CDS             complement(51..311)
                     /locus_tag="PLZ72_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36383.1"
                     /translation="MTDPSDHADLEHDPNDPDGTRGHAGASVTVPTTIVCVDCGGTCH
                     LLTAPFDDGVGPVGFRPGDIVSYRCADCLDRWDVELDESDVN"
     gene            complement(314..1090)
                     /locus_tag="PLZ72_08555"
     CDS             complement(314..1090)
                     /locus_tag="PLZ72_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA36384.1"
                     /translation="MADDAAPPLPGSPGAPLWDPLAVPAATVIVLRDGTDGPEALMLK
                     RDRDLSFAGGMWVFPGGRIDDTDFDPEAPGNPDDADEAVENAARRAAAREAIEESGVE
                     VDPTALRRWSHWTPPPLQDKRFSTAFFVAPAVAADVRVTIDDGEIRAHRWARPDEVIR
                     RRDAGEVALAPPTFITLTQLLRHDSVARILDAAPTGPIEHFSTRVALRDGGWTAVYHG
                     DSTFDELGDLGGMGEVDLDAPGPRHRLVMAERWSYQREGC"
     gene            1433..4000
                     /locus_tag="PLZ72_08560"
     CDS             1433..4000
                     /locus_tag="PLZ72_08560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNA36385.1"
                     /translation="MALDQDVVSTTESIDTAAAGRPVQVVGDTPAPPTSTGLVDLTDS
                     ATGSPMQVRKRDGSLEPVDLNKIVRAVARCAVGLDNVDPMRVATRTISGLVDHSSTEE
                     LDELSIRTAAALISEEPNYSRLAARLLATVIDKEVQNQDIYSFSQSVAMGHAQGIIGD
                     ETAAMVATNARKLNDAVLADRNWLYEFFGLRTVYDRYLLRHPESRRVVETPQYFLLRV
                     ACGLSRTPEEAIEFYELISSLEYLPSSPTLFNSGTAHPQMSSCYLLDSPEDNLDAIYD
                     RYRDVARLSKHAGGIGLSYSRVRSRGSLIRGTNGLSNGIVPWLKTLDSSVAAVNQGGR
                     RKGAACVYLETWHADIEEFLELRDNVGDTARRTLNLNLANWVPDLFMERVEKDWQWSL
                     FDPKKVPHLIDLYGDEFRDAYIQAENDGLFERQVPARQIYQRMMRTLAETGNGWMTFK
                     DASNLKCNQTGTTKADGTPNVVHLSNLCTEILEVTNQAETAVCNLGSVNLGAMVRPGA
                     DGAPAFDFDRLAEVVRLAVPFLDRVVDINFYPTDAAGRSNSKWRPVGLGLMGLQDVFF
                     KLGLPFDSDAAKDLSTRISEEIYFNALTASADLAEAHGAHDGFAETRAASGQLQFDLW
                     GVTPTDAARWDALKARIAEVGLRNSLMIAIAPTATIASIAGCYECIEPQVSNLFKRET
                     LSGEFLQINTYLVRELQARGLWTDSIISAIKRDEGSVQGIDAIPADVRAVYRTAWELP
                     MRALLDMGAARGAYIDQSQSLNLFMESPTIGKLSSMYVHAWKSGLKTTYYLRSRPATR
                     INKTTTGATTSSTPDGSGPGGPGGPGGGEPAPAPTAYSDEEALACSLENPEACEACQ"
     gene            4088..5188
                     /locus_tag="PLZ72_08565"
     CDS             4088..5188
                     /locus_tag="PLZ72_08565"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNA36386.1"
                     /translation="MALNEAVSLDPDLFDPAVTSGTVAPTAGTAPAAGQLLDPGFHLT
                     LRPMRYPQFYEMYRAAIRNTWTVEEIDFSDDLVDLQRKLLPAERHLINRLVAFFATGD
                     SIVSNNLVLNLYQHINTPEARMYLSRQLYEEALHVQFYLTLLDTYIPDQDERAQAFAA
                     IDNIPSIRRKGEFCFKWIDSIHGLAELRTREERKQFLLNLICFAACIEGLFFFGAFAY
                     VYFLRSKGLLNGLASGTNWVFRDESCHMNFAFEVIEQVRREEPDLFDDDLGARVTEMI
                     SEAVECEFGFAEDLLSQGVAGMSLVDMREYLQYVADQRLLTLGLSKVYGSKNPFAFME
                     LQDVQELTNFFERTVSSYQVGVSGDVAFDEDF"
     gene            complement(5195..6262)
                     /locus_tag="PLZ72_08570"
     CDS             complement(5195..6262)
                     /locus_tag="PLZ72_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA36387.1"
                     /translation="MIRRSRSTRSTRDGGGDATTVALAGAGAIAVVHALAAPSAGCRV
                     TAVASAGGSSARHLAGQLDEQVRHAVRAVPIDDLPAGADLLVVASPPDSHVALALQGL
                     AAGADVLVEKPLATNLADADRLVDAARAATASGVVLRCAENLLHAPSWAVITGHRSAM
                     GPLQHLSARTLQPPPSWGHFTRPLTAGGVLFDLGPHALALVMALAGEPVVSVTGELSS
                     TRDDGADDDASVQLRFASGLVATVEVSWTAPEVEWSLQAASADGVARLELSPDVLVEI
                     DGEPVAVPDRVADAADPALERMGYIGQLARLEESQTVETARDVLEVICAAYASAGDGG
                     TEVPVPFAGDRTLTPMQLWQG"
     gene            complement(6273..7205)
                     /locus_tag="PLZ72_08575"
     CDS             complement(6273..7205)
                     /locus_tag="PLZ72_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA36388.1"
                     /translation="MAHRFRFGIQTTRPFEGMTWSETARRIEELGYSTLFVPDHFGDQ
                     LAPIAALSVAAEATTTLKVGALVFDNDYRHPVTLAKEMATLDLLSSGRVELGLGAGWM
                     VTDYEQSGMTYDPPAVRVDRFEEALAVVQGLFGDGPVDFDGEHYRISGLEGLPRPHTP
                     GGPPLLIGGGGRRVLSIAARHADIVGVNPDLRAGRIDAATTADALASAVDRKIAWVRQ
                     AAGDRIEQIELNQLCFAATITDDLDPYVEFLAGLFGTPADEVLESPTVVAGSLPEIVD
                     RLEARRDRWGFSYHVFQGDAAEVMAPVVAELSGR"
     gene            7349..9616
                     /locus_tag="PLZ72_08580"
     CDS             7349..9616
                     /locus_tag="PLZ72_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNA36389.1"
                     /translation="MNRPTRLLLRFTAIVVLGVSALALVAAALLPQIGKLPEAASFTA
                     RTKIALPSLPEASHIVTQSGEQYGILAGVENRDVVTLDQISPELQKTVLAVEDADFYD
                     HDGVSVRSILRAFRANSDAGTISQGGSTITQQLVKLSLVGDERNFTRKMKEASLAVQL
                     EQQLCDGVAKKVCKDQILEQYLNTVYLGRGAYGVQAGAQTYFDKPASQINYAEAAVLA
                     SLIRYPNGYDPIRFPKVAKERRLVVLKRMQDQGLIDHNQATFIASLPLPTRAYGRPVA
                     TTTESLTYVERKVRDELVKAEWLAPTEELRRYLVFNGGLRITTTIDPQMQAAAEAAAA
                     TNPLHAANPETAVALATVEPATGAVRAIVGEADVPGKGLVETAAPLGGRSSGSSFKVF
                     TLVAALEAGYSINSTVSGAYAPDNMKKLWGVKESGPYPDDCPSQGMVDLGRALAQSNN
                     CAFMRLQGAVGFDAVKDTAVKLGVTASSLDPNHVEPACFTIGCDALVKPLDMALAYAT
                     IANDGRRNDAHFVSKVEDRTGKVIFEAKPRNEQVIPVDVARQAIVGMEKVVTSGTYSA
                     GSLPQRRPAAGKTGTTEVEGGRNTDVWFIGFTPQLSTAVWIGNPAANTHMKGGKVQGG
                     ATAARVWHAFMSDVLDGAPVLDFPEADKLPKAKAVPDPWKGSSSKSTGSTSKSSTRSR
                     SRSTTIAPDSGTGGSGSSGSGSSGHGTGGGSGGDDGAGGGNGGGTGAGGGTGTGTGTG
                     GTGTGGGGGGAGAGG"
     gene            9646..10359
                     /locus_tag="PLZ72_08585"
     CDS             9646..10359
                     /locus_tag="PLZ72_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNA36390.1"
                     /translation="MGSLDGLIDVQTLDTRADQVRHRRDHLPQAEAVAEARRDRDAAD
                     ARAETARGRLRELVKREKALEDEAALVEDKAAEIDRKLYDGSVVAHKELEAFQADHRL
                     LKARQSELEDEAIEIMEAAEPIQAEVDALESEVAGLDARLDALTAEMDAARSELDGEL
                     ATLERERAVAAGAVPDDVLVAYESTRSRLGGVGAARLLGNRCEGCHLEIPSAELEALR
                     HAPEDALVLCPECDRILVR"
     gene            10384..10896
                     /locus_tag="PLZ72_08590"
     CDS             10384..10896
                     /locus_tag="PLZ72_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36391.1"
                     /translation="MFIWFAVLGVVITWAVFQSPAIDYRLVALGSVLPVVEVPFGAGP
                     LHALVAPTAVLVVVMAATVRRRLVRRRWLGLPIGMYMHLVVDLAFTLTATFWWPFLGW
                     SFTPGPAPELDRGIWSVVMELAGLALGWWAWRRFGLDDAQRRSRFLRTGQLDRAVMGG
                     PGPRSGGRTP"
     gene            10893..11489
                     /locus_tag="PLZ72_08595"
     CDS             10893..11489
                     /locus_tag="PLZ72_08595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA36392.1"
                     /translation="MSSLVVVRHGRTAANAAGLLLGRLDVDLDALGRRQAVALAAAVT
                     ASSGPIRTVVSSPLRRAVETAEAFGLPVEVDERFIEVDYGDWDGRPLSEVPPEVWSEW
                     RSDPHFSPTAGESLHGVAARVDEACRDWAARAGDGSVVVATHVSPVKAAVGWALGADV
                     NWTSHVDPASITRIDVDGGRPRLRSFNETGHLVGLAAG"
     gene            11557..12321
                     /locus_tag="PLZ72_08600"
     CDS             11557..12321
                     /locus_tag="PLZ72_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36393.1"
                     /translation="MELLDEESHRILLWAVRLHEVGHAPSRAELTGLAEGRRPAGLAT
                     VTGLTGRHSEFQEGSVESLLRRGLLQSVGASGMAPTELGRRVIDALGLEDGFPTFEVL
                     DADLSSSDPLAFARIVGRVASLHRPMVVDPYCRRSELEYLVAHTSVTRVLVSDRLDDE
                     ELAGLVEHVRSLGHRDLKLRIRVAAAEEIQDRCVVSRDRVLQVGGLPSAVGGGASVIS
                     EPHDLGEQTREHYRAVWRSATRLVSHRPRRGRSRAA"
     gene            12413..13708
                     /locus_tag="PLZ72_08605"
     CDS             12413..13708
                     /locus_tag="PLZ72_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36394.1"
                     /translation="MRKNVTRALIVAIAALGLIASACGGDDESSDKSTTTTEASNSGG
                     DRGNVDGQLKLGNLVPQTGDLNVIVKSLETPVAMAVKAINDAGGVNGKPVELVVADDG
                     TNPNVAATSYAKLINTDKVDAIIGPAPSGVAAKMLNSFGTDKVPTCSGSTTAANLSGG
                     GDGYFFRTAPGDDLQGPALAALITGDKHEKVAILARNDDYGKGFSESLSKALESSGAQ
                     VTETVLYNPDASNFDADVQKALDSSPDAVVILGFNDDGAKVVSTLIGKGAGPSQMPLY
                     TADGMKGSSFGKTVDPSDPSKVEGIRGTAPASAPQGVESPFTAEFAKTGIDTIFSSYY
                     WDCTNLMALAAVKAKSDSGEAIAENFAANLEGDNDCNNFKDCKALLEEGKSIHYRGAS
                     SAFEKWNKMEPGTGVYDVWAYTAEGKDANVEGAAQIKIG"
     gene            complement(13831..14562)
                     /locus_tag="PLZ72_08610"
     CDS             complement(13831..14562)
                     /locus_tag="PLZ72_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36395.1"
                     /translation="MTGEPLLVVRDLVAGYVAGVDILRGTSIDVARSELVGIIGPNGA
                     GKSTLIKAVFGLVPVRSGTMTLDGDDIVGVHGYDLVSRGVGYVPQVRNVFGTLSVEEN
                     LRMGVYLRPDRFAARRDDVLDLFPTLRDRRGDRADALSGGQRQVLAMARALMMEPSLL
                     LLDEPSAGLSPAMQDEVFARIAAINHAGVSVLIVEQNAHKALRICHRGYVLDNGTDAY
                     TGTGAELLEDPKVIELYLGSLVRNR"
     gene            complement(14559..>14891)
                     /locus_tag="PLZ72_08615"
     CDS             complement(14559..>14891)
                     /locus_tag="PLZ72_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36396.1"
                     /translation="QLLLDKIRELPGEGITVLFVEHDMDVVSAISDVVVCMAEGRIIA
                     SGTAGQVASDPRVIDAYLGGSSGDLRDMADELAPVVDELYADGRTPDSTDSDGTDSDG
                     TDSDGARP"
BASE COUNT         2267 a         5270 c         5232 g         2122 t
ORIGIN      
        1 ctcctcaccg ccgcctgagg cgtcggctcc gccgatcggc ggaaccagga tcagttcacg
       61 tcggactcgt cgagctcgac gtcccagcgg tccaggcagt cggcgcagcg gtacgacacg
      121 atgtcgccgg gccggaaccc gaccgggccg accccgtcgt cgaacggggc ggtcagcagg
      181 tggcaggtcc caccgcagtc gacgcacacg atcgtcgtcg gcacggtgac cgacgccccg
      241 gcgtgtcccc gggtcccgtc ggggtcgttg ggatcgtgct cgaggtcggc gtgatcggac
      301 gggtcggtca tcctcagcac ccctcgcgct ggtaggacca gcgctcggcc atgaccagcc
      361 ggtgccgggg tccgggggcg tcgaggtcga cctcgcccat gccgcccagg tctccgagct
      421 cgtcgaaggt gctgtcgccg tggtacacgg cggtccaccc gccgtcgcgc agcgcgaccc
      481 gggtcgagaa gtgctcgatc ggaccggtcg gcgctgcgtc gaggatccga gccaccgagt
      541 cgtggcggag cagctgcgtc agggtgatga acgtcggcgg ggcaagcgcg acctcgccgg
      601 cgtcgcggcg gcggatcacc tcgtcgggtc gggcccatcg gtgcgcgcgg atctccccgt
      661 cgtcgatggt gacccggacg tcggcggcga cggcgggggc cacgaagaag gcggtggaga
      721 agcgcttgtc ctgcagtggt ggaggtgtcc agtgggacca ccgtcgaagt gcggtcggat
      781 cgacctcgac cccggactcc tcgatcgcct cccgggccgc ggcccgacgc gccgcgttct
      841 cgaccgcctc gtcggcatcg tcgggattcc cgggagcttc ggggtcgaaa tcggtgtcgt
      901 cgatgcgacc tccggggaac acccacatcc cgccggcgaa gctgaggtcg cggtcccgct
      961 tcagcatgag cgcctcgggt ccgtcggtgc cgtcgcgcag cacgatgacg gtggcggccg
     1021 gcaccgcgag cgggtcccac agcggggctc cgggggagcc cggcagcggg ggagcggcgt
     1081 cgtcggccat cgccccaccc taggacgcgc ccgggccccg aaccgcgggg atcgatgccg
     1141 aactcgcgcg gagagtggca atcgtcacgt ttctgagggt gttccggtgg acgcggagcg
     1201 aggttttcgg aaagcgttga cacctctgta gttacctggc tgtaattacg gccctgtccc
     1261 cacatcttgg gggtggggga acgctcggcc acaacatctg gtgccagtcc gaccgctggt
     1321 cgcacggccc gaggtcggcc cgagggtccc gcagcacgtg atgagaaccc tccggttcgg
     1381 gcgaaccgga gacaccgaca ccagcgcagg gagcgcagaa gggggacgag tcgtggcact
     1441 cgaccaggac gtcgtcagca caaccgagag catcgacacc gctgcggccg gacgcccggt
     1501 gcaggtggtg ggcgacaccc ccgcgccgcc gacgtcgacc ggtctggtcg acctcaccga
     1561 ctcggccacc ggatccccga tgcaggttcg caagcgcgac ggcagcctgg agcccgtcga
     1621 tctcaacaag atcgtccgcg ccgtcgcccg atgcgcggtg ggactcgaca acgtcgaccc
     1681 gatgcgggtc gccacccgca ccatctcggg cctggtcgac cactcgagca ccgaggagct
     1741 cgacgagctg tcgatccgca ccgcggccgc gctgatctcc gaggagccga actactcgcg
     1801 cctggcggcc cggctgctcg cgacggtgat cgacaaggag gtccagaacc aggacatcta
     1861 ctccttctcc cagtcggtcg ccatgggcca cgcccagggg atcatcggcg acgagaccgc
     1921 ggcgatggtc gccaccaacg cccgcaagct caacgatgcc gtgctggccg atcgcaactg
     1981 gctctacgag ttcttcggcc tgcggaccgt gtacgaccgc tacctgctgc gccaccccga
     2041 gtcccgtcgg gtcgtcgaga cgccgcagta cttcctgctg cgcgtcgcct gtggcctgtc
     2101 gcgcaccccg gaggaggcga tcgagttcta cgagctgatc tccagcctgg agtacctgcc
     2161 gagctcgccg acgctgttca actcgggaac cgcccatccg cagatgtcga gctgctacct
     2221 gctcgactcg cccgaggaca acctcgacgc catctacgac cgctaccgcg acgtcgcccg
     2281 cctgtccaag cacgccggcg gcatcgggct gtcctacagc cgggtccggt cccgcggctc
     2341 gctgatccgg ggcaccaacg gcctctccaa cggcatcgtg ccgtggctca agaccctcga
     2401 cagctccgtc gcggcggtca accagggcgg ccggcgcaag ggcgccgcgt gcgtctacct
     2461 cgagacctgg cacgccgaca tcgaggagtt cctcgagctg cgcgacaacg tcggcgacac
     2521 cgcccggcgc acgctcaacc tgaacctggc caactgggtc cccgacctgt tcatggagcg
     2581 ggtcgagaag gactggcagt ggtcgctgtt cgaccccaag aaggtcccgc acctgatcga
     2641 cctctacggc gacgagttcc gcgacgccta catccaggcc gagaacgacg gcctgttcga
     2701 gcgtcaggtc ccggcccgtc agatctacca gcggatgatg cgcaccctgg ccgagaccgg
     2761 caacggctgg atgacgttca aggacgcatc caacctcaag tgcaaccaga ccgggaccac
     2821 caaggccgac ggcaccccca acgtcgtgca cctgtccaac ctctgcaccg agatcctcga
     2881 ggtcaccaac caggccgaga ccgcggtgtg caacctgggc tcggtcaacc tcggcgccat
     2941 ggtgcgtccg ggtgccgacg gcgcccccgc gttcgacttc gaccggttgg ccgaggtggt
     3001 ccgcctggcg gtgccgttcc tcgaccgggt cgtcgacatc aacttctacc cgaccgacgc
     3061 cgccggccgc tccaactcca agtggcgtcc ggtcggcctg gggctgatgg gcctgcagga
     3121 cgtgttcttc aagctcggcc tgccgttcga ctccgacgcg gcgaaggacc tgtcgacccg
     3181 catctccgag gagatctact tcaacgccct gaccgcgtcc gcggacctgg ccgaggccca
     3241 cggcgcccac gacggcttcg ccgagacccg cgccgcgtcg ggccagctcc agttcgacct
     3301 gtggggcgtc accccgaccg acgccgcccg ctgggacgcc ctcaaggcgc gcatcgccga
     3361 ggtcggcctc cgcaactcgc tgatgatcgc catcgcgccg accgcgacga tcgcgtcgat
     3421 cgccggctgc tacgagtgca tcgagccgca ggtgtcgaac ctgttcaagc gcgagacgct
     3481 ctcgggcgag ttccttcaga tcaacaccta cctggtacgc gagctgcagg cccgcggcct
     3541 gtggaccgat tcgatcatct cggcgatcaa gcgcgacgag ggatcggtcc agggcatcga
     3601 cgccatcccc gcggacgtcc gggccgtgta ccgcaccgcc tgggagctgc cgatgcgggc
     3661 cctgctcgac atgggcgccg cccgcggcgc gtacatcgac cagagccagt cgctgaacct
     3721 gttcatggag tcgccgacca tcgggaagct gtcgtcgatg tacgtccatg cctggaagtc
     3781 cgggctgaag accacgtact acctgcgttc ccgtccggcg acccgcatca acaagacgac
     3841 gacgggagcg acgacgtcga gcacgccgga cggctcgggt cccggtggac cgggaggccc
     3901 ggggggcggc gaacccgcgc cggcgcccac cgcctactcc gacgaagagg ccttggcgtg
     3961 ctcgctcgag aaccccgagg cgtgcgaggc ctgccagtga gcaccgctcg ctgagcagtc
     4021 cgccgactcc gaccccacct tcttccgacc cgccccactc gcccgcaccc aaggagcacc
     4081 gcgtcccatg gccctgaacg aagccgtctc gctcgacccc gacctgttcg accccgccgt
     4141 cacatcgggc accgttgccc cgaccgccgg caccgcgccc gccgcgggtc agctgctcga
     4201 ccccgggttc cacctcaccc tgcggcccat gcgctacccg cagttctacg agatgtaccg
     4261 cgccgcgatc cgcaacacct ggaccgtcga ggagatcgac ttctccgacg acctcgtcga
     4321 cctccagcgc aagctgctgc ccgccgagcg gcacctgatc aaccgcctgg tcgcgttctt
     4381 cgccaccggc gactcgatcg tgtcgaacaa cctggtgctg aacctgtacc agcacatcaa
     4441 cacgcccgaa gcccggatgt acctgagccg ccagctctac gaggaggcgc tgcacgtcca
     4501 gttctacctg acgctgctcg acacctacat cccggaccag gacgagcggg cgcaggcgtt
     4561 cgcggcgatc gacaacatcc cgtcgatccg ccgcaagggc gagttctgct tcaagtggat
     4621 cgactcgatc cacggcctgg ccgagctgcg cacccgcgag gagcgcaagc agttcctgtt
     4681 gaacctgatc tgcttcgcgg cctgcatcga ggggctgttc ttcttcgggg cgttcgccta
     4741 cgtctacttc ctgcgctcca agggcctgct caacgggttg gcgtcgggca ccaactgggt
     4801 gttccgcgac gagtcctgcc acatgaactt cgccttcgag gtgatcgagc aggtccgacg
     4861 cgaggagccc gacctgttcg acgacgacct cggcgcccgg gtcaccgaga tgatctccga
     4921 ggcggtcgag tgcgagttcg gcttcgccga ggacctcctg agccagggcg tggccggcat
     4981 gtcgctggtc gacatgcgcg agtacctgca gtacgtggcc gaccagcggc tgctcaccct
     5041 ggggctgtcc aaggtgtacg gatcgaagaa cccgttcgcc ttcatggagc tccaggacgt
     5101 ccaggagctg accaacttct tcgagcggac cgtgtcgtcg taccaggtgg gcgtgtccgg
     5161 cgacgtcgcc ttcgacgagg acttctgagc gggctcagcc ctgccacagc tgcatcgggg
     5221 tgagcgtccg gtcgccggcg aacggcaccg gcacctcggt cccgccgtcg ccggccgacg
     5281 cgtacgcggc gcagatcacc tcgagcacgt cgcgggccgt ctcgacggtc tgggactcct
     5341 cgaggcgggc cagctgtccg atgtagccca tccgctccaa cgccgggtcc gccgcgtcgg
     5401 cgacccggtc cgggaccgcc accggctcgc cgtcgatctc gaccagcacg tcgggcgaga
     5461 gctccaggcg ggcgacgccg tcggccgacg cggcctgcag cgaccactcc acctccggtg
     5521 cggtccacga cacctcgacc gtggcgacca gtccggaggc gaagcgcaac tgcaccgacg
     5581 cgtcgtcgtc ggcgccgtcg tcgcgggtgg aggacagctc gccggtcacc gacacgaccg
     5641 gctcgcctgc cagcgccatc accagcgcca gggcgtgcgg gccgaggtcg aacaggacgc
     5701 cgccggcggt gagcggtcgg gtgaagtgcc cccaactcgg cggcggctgc agggtccggg
     5761 ccgacaggtg ctgcaacggc cccatggcgg agcggtgccc ggtgatcacc gcccagctcg
     5821 gggcgtgcag caggttctcg gcgcatcgga ggaccacccc ggacgcggtg gcggcccggg
     5881 cggcgtcgac gaggcggtcg gcgtcggcca ggttcgtcgc cagcggcttc tcgaccagca
     5941 cgtcggctcc cgcggccagg ccctgcaggg cgagagcgac gtgggagtcc ggcgggctgg
     6001 cgacgaccag caggtcggcc ccggcgggca ggtcgtcgat cggcacggcg cgcaccgcgt
     6061 ggcgcacctg ctcgtcgagc tggcccgcca ggtgccgggc cgacgaaccg ccggccgatg
     6121 ccaccgcggt cacccgacag ccggcggacg gcgccgccag ggcgtgcacg acggcgatgg
     6181 cgccggcccc ggcgagcgcg acggtggtgg cgtcaccgcc gccgtcgcgg gtggagcggg
     6241 tggagcgcga ccgccggatc acggggtgac cgtcagcgcc cgctcagctc ggccacgacc
     6301 ggggccatga cctcggccgc gtcgccctgg aacacgtggt agctgaaccc ccagcggtcc
     6361 cgtcgggctt ccaggcggtc gacgatctcg ggaagggacc cggcgaccac ggtcggcgac
     6421 tcgagcacct cgtcggccgg ggtgccgaac agcccggcca ggaactcgac gtacgggtcg
     6481 aggtcatcgg tgatggtggc cgcgaagcac agctggttga gctcgatctg ctcgatgcgg
     6541 tccccggcgg cctggcggac ccaggcgatc ttgcggtcga ccgccgaggc gagcgcgtcg
     6601 gcggtcgtcg ccgcgtcgat ccgaccggcg cgcaggtcgg ggttgacccc gacgatgtcg
     6661 gcgtggcgag ccgcgatcga cagcaccctc cgtcctccac cgccgatcag cagcggggga
     6721 ccgccggggg tgtgggggcg aggcaggcct tccagacccg agatccggta gtgctcgccg
     6781 tcgaagtcga ccgggccgtc gccgaacagg ccctgaacga cggcaagcgc ctcttcgaag
     6841 cggtcgacgc ggaccgcggg cgggtcgtag gtcatgccgg actgctcgta gtcggtgacc
     6901 atccagccgg cgccgagccc gagctcgacc cggcccgagc tgagcaggtc cagggtggcc
     6961 atctccttgg ccagcgtgac cgggtgtcgg tagtcgttgt cgaacaccag ggcgccgacc
     7021 ttcagcgtgg tggtggcctc ggctgccacc gacagcgccg cgatcggcgc gagctggtcg
     7081 ccgaagtggt cggggacgaa cagggtgctg tagccgagct cctcgatccg tcgggcggtc
     7141 tcggaccacg tcatcccctc gaaggggcgg gtggtctgga tgccgaagcg gaaccgatgg
     7201 gccatggccg ggcacgctac cggtcgaccc tgcggatctg cgtccctgag gaactgctcg
     7261 gtcatcgccg agcacgcccg tcggcgcccc gaccgggccg tcggcggccg gtagcgtggg
     7321 acggagccgc tgccggcgac cccctgccat gaaccgaccg acccgactcc tcctgcgctt
     7381 caccgcgatc gtcgtgctgg gcgtcagcgc gctcgccctg gtcgcggcgg ctctcctgcc
     7441 ccagatcggc aagctccccg aggccgcgtc gttcaccgcc cggaccaaga tcgcgctgcc
     7501 gtccctgccg gaggcctccc acatcgtcac gcagtccggc gagcagtacg ggatcctggc
     7561 cggggtggaa aaccgcgacg tggtcacact cgaccagatc tcgcccgagc tgcagaagac
     7621 ggtgctggcg gtggaggacg ccgacttcta cgaccacgac ggcgtgtcgg ttcgatcgat
     7681 cctgcgggcg ttccgggcca acagcgacgc gggcaccatc tcccagggcg gctcgacgat
     7741 cacccagcag ctggtcaagc tctccctggt cggcgacgag cggaacttca cccgcaagat
     7801 gaaggaggcg tccctcgcgg tccagctcga gcagcagctc tgcgacggtg tggccaagaa
     7861 ggtgtgcaag gaccagatcc tcgagcagta cctcaacacc gtctacctgg gtcgcggcgc
     7921 gtacggggtg caggccggcg cccagaccta cttcgacaag ccggcgtccc agatcaacta
     7981 cgcggaggcc gcggtgctgg catcgctgat ccgctacccg aacgggtacg acccgatccg
     8041 cttcccgaag gtggccaagg agcgccggct ggtcgtgctc aagcggatgc aggaccaggg
     8101 tctgatcgac cacaaccagg ccacgttcat cgcctcgctc ccgcttccca ccagggccta
     8161 cggccggccg gtggccacca ccaccgagtc gctgacctac gtcgagcgca aggtccgtga
     8221 cgagctggtg aaggccgagt ggctggcgcc gaccgaggag ctgcgccgct acctggtgtt
     8281 caacggcggg ctgcgcatca ccaccaccat cgatccgcag atgcaggcgg cggccgaggc
     8341 tgcggccgcg acgaacccgc tgcatgcggc caaccccgag accgcggtgg cgctggccac
     8401 ggtcgagccc gccaccggag cggtccgggc gatcgtcggt gaggccgacg tccccggcaa
     8461 gggcctggtc gagaccgcgg ccccgctcgg cgggcgctcg tcgggatcgt cgttcaaggt
     8521 gttcaccctg gtcgccgctc tcgaagccgg ctacagcatc aacagcaccg tgtcgggggc
     8581 ctatgccccc gacaacatga agaagctgtg gggggtcaag gagtcgggtc cctaccccga
     8641 cgactgcccg tcgcagggga tggtcgacct gggtcgggcc ctggcgcagt cgaacaactg
     8701 cgcgttcatg cgcctgcagg gcgcggtggg cttcgacgcg gtcaaggaca cggcggtgaa
     8761 gctgggcgtc accgcgtcgt cgctcgatcc gaaccacgtc gagccggcgt gcttcaccat
     8821 cggctgcgac gccctggtca agccgctcga catggccctg gcctacgcca cgatcgccaa
     8881 cgacggacgc cgcaacgacg cccacttcgt gtcgaaggtg gaggaccgca ccggcaaggt
     8941 gatcttcgag gccaagcccc gcaacgaaca ggtcatcccc gtcgacgtcg cccgtcaggc
     9001 catcgtcggc atggagaagg tcgtcaccag cggcacctac tccgccgggt ccctgcccca
     9061 gcggcgcccg gcggccggca agaccggcac caccgaggtc gagggcggcc ggaacaccga
     9121 cgtgtggttc atcgggttca ccccgcagct gtcgaccgcg gtgtggatcg gcaacccggc
     9181 ggccaacacc cacatgaagg gcggcaaggt tcagggtggt gccaccgcag cccgggtctg
     9241 gcacgcgttc atgtccgacg tcctcgacgg cgccccggtg ctcgacttcc ccgaggccga
     9301 caagctcccc aaggccaagg ccgtgcccga tccgtggaag ggatcgtcgt cgaagtcgac
     9361 cggctcgacg tcgaagtcct cgacccggtc gcggtcgcgt tccacgacca ttgcgccgga
     9421 ctccggcacc ggagggtccg ggtcgagcgg gtccgggagc tccggccacg gcaccggcgg
     9481 tgggtccgga ggtgacgacg gtgcaggggg cggcaacggc ggtggcaccg gtgcgggcgg
     9541 cggtaccggt accgggacgg ggaccggagg gacgggaacc ggtggcggcg ggggcggagc
     9601 cggtgccggc ggctgagctt cggtccgccc tgtaggttct gcggcgtggg atcgctcgac
     9661 ggcctgatcg acgtgcagac gctggacacc cgggccgatc aggtccggca tcggcgtgat
     9721 cacctcccgc aggcagaggc cgtggccgag gcccgtcgcg accgcgacgc cgcggacgcc
     9781 agggccgaga ccgcccgggg tcggctccgc gagctggtca agcgcgagaa ggcgctggag
     9841 gacgaagccg ctctggtcga ggacaaggcg gccgagatcg accggaagct ctacgacgga
     9901 agcgtggtcg cccacaagga gctcgaggcg ttccaggccg accaccgact gctcaaggcc
     9961 cgccagtccg agctcgagga cgaggccatc gagatcatgg aagcggccga acccatccag
    10021 gccgaggtcg acgcgctcga atccgaggtc gccggtctcg acgcccgcct ggacgccctg
    10081 accgccgaga tggacgcggc gcgatccgag ctcgacggcg agctcgcgac actggagcgt
    10141 gagagggccg tggcggccgg tgcggtgccc gacgatgtgc tcgtcgccta cgagagcacc
    10201 cgcagccgcc tgggcggggt gggggcggcc cgactgctcg gcaaccgctg cgaggggtgc
    10261 cacctggaga tcccgtcggc cgagctggaa gcgctgcgac acgcccccga ggacgcgttg
    10321 gtgctgtgtc cggagtgcga ccgcatcctg gtgcgctgag cgctgcgggg taggtgcgcg
    10381 gtcgtgttca tctggttcgc ggtcctcggg gtggtgatca cctgggcggt gttccagagc
    10441 ccggcgatcg actaccggct ggtggccctc ggctcggtgc tgcccgtggt cgaggttccc
    10501 ttcggtgccg gcccgctgca cgccctggtg gcgccgacgg cggtgctcgt ggtcgtgatg
    10561 gcggccacgg tccgtcggcg tctggtgcga cgccggtggt tgggtctgcc gatcggcatg
    10621 tacatgcacc tggtcgtcga cctggcgttc acgctgaccg ccacgttctg gtggcctttc
    10681 ctgggctggt cgttcacccc cggtcccgca cccgagctcg accgtggtat ctggagcgtg
    10741 gtgatggagc tcgccggcct cgcgctcggg tggtgggcgt ggaggcgctt cgggctcgac
    10801 gacgcgcagc ggcggtcacg gttcctgcgc accggtcagc tcgaccgggc ggtcatgggt
    10861 ggcccgggcc cccgttcggg ggggaggacg ccgtgagctc gctggtcgtc gtgcgtcacg
    10921 gtcggacggc ggccaacgcg gcggggctgc tgctcggacg gctcgacgtg gacctggacg
    10981 cgctcggacg acgccaggcg gtcgcactgg ccgccgcggt gaccgcgtcg agcggaccga
    11041 tccggacggt ggtgtccagt ccgttgcgtc gggcggtgga gaccgccgag gcgttcgggc
    11101 tgcccgtcga ggtcgacgag cgcttcatcg aggtcgacta cggcgactgg gacggccgac
    11161 cgctgtccga ggtcccaccc gaggtttggt cggagtggcg ctcggacccg cacttcagcc
    11221 cgaccgcggg cgagtccctg cacggcgtgg cggcgagggt cgacgaggcg tgccgggact
    11281 gggccgctcg agccggggac ggatcggtgg tcgtcgccac ccacgtgtcg ccggtcaagg
    11341 cggctgtcgg ctgggcgctc ggtgcggacg tgaactggac cagtcacgtc gacccggcgt
    11401 cgatcacccg catcgacgtc gacggcggcc gaccccggct gcggtccttc aacgagaccg
    11461 gtcacctggt cggactcgcc gcgggctgag cccgatatga cgacggtcac ccccagagtc
    11521 gccggttcgt cgcgccaccc ctctaggttg gggggaatgg agttgttgga cgaggagtct
    11581 catcggatcc ttctgtgggc ggtccgcctg cacgaggtgg gacacgctcc gagccgcgcc
    11641 gagctgacgg gactcgccga gggccgccga cccgccgggc tcgccacggt caccgggttg
    11701 accggtcggc actccgagtt ccaggagggg tcggtggagt cgttgctgcg ccggggtctc
    11761 ctgcagtccg tcggcgcgtc cggcatggcc ccgaccgagc tggggcgtcg ggtgatcgac
    11821 gccctcgggc tcgaggacgg cttcccgacc ttcgaggtgc tcgacgcgga cctcagctcg
    11881 agcgatccgc tggccttcgc ccgaatcgtc ggtcgggtcg cgtcgctgca ccgaccgatg
    11941 gtcgtcgacc cgtactgccg tcgcagcgag ctcgagtacc tggtcgccca cacgtcggtc
    12001 acacgggtgc tggtcagcga ccgcctcgac gacgaggagc tcgccggcct ggtggagcac
    12061 gtccggtccc tgggccaccg cgacctgaag ctgcgcatcc gcgtcgcggc ggccgaagag
    12121 atccaggacc gctgcgtcgt cagtcgggac cgggtcctgc aggtgggcgg tctgccgtcg
    12181 gctgtcggag gcggagcgtc ggtcatctcc gagccccacg acctcggcga gcagacccgc
    12241 gagcactacc gcgccgtgtg gcgatccgcg acccgcctgg tcagccaccg gccccgtcgc
    12301 ggtcgatccc gcgccgcctg agcgcgaaag acgtcaccgg cgactctctc gaacctaggg
    12361 tggtcggacc cggacacggc gtccgggggg acgatccgag gagacacggg tgatgcgcaa
    12421 gaacgtcacg cgagcgctca tcgtcgccat cgcggcactg gggctgatcg cctcggcctg
    12481 cgggggcgac gacgagtcga gcgacaagtc cacgaccacg acagaggcgt cgaacagcgg
    12541 cggcgaccgc ggcaacgtcg acggccagct gaagctgggc aacctcgtcc cccagaccgg
    12601 cgacctgaac gtcatcgtca agtcgttgga gacgccggtg gccatggcgg tcaaggcgat
    12661 caacgacgcc ggcggcgtca acggcaagcc ggtggagctc gtcgtcgccg atgacggcac
    12721 caacccgaac gtggccgcca ccagctacgc caagctgatc aacaccgaca aggtcgacgc
    12781 catcatcggc ccggcaccgt cgggtgtggc cgccaagatg ctcaactcgt tcggcaccga
    12841 caaggtcccg acctgctccg gatcgaccac cgcggccaac ctgagcggcg gcggcgacgg
    12901 ctacttcttc cggaccgccc ccggcgacga cctccagggc ccggcgctcg ccgccctcat
    12961 caccggtgac aagcacgaga aggtggcgat ccttgcccgc aacgacgact acggcaaggg
    13021 cttctcggag tccctgtcca aggcgctcga gtcgagcggc gcgcaggtca ccgagaccgt
    13081 gctgtacaac cccgacgcct cgaacttcga cgcggacgtc cagaaggccc tcgactcgtc
    13141 gcccgacgcc gtggtcatcc tcggcttcaa cgacgacggc gccaaggtcg tcagcacgct
    13201 gatcggcaag ggcgccggcc cgtcgcagat gccgctgtac accgcggacg ggatgaaggg
    13261 ctcgagcttc ggcaagaccg tcgacccgtc ggacccgtcc aaggtcgagg gcatccgcgg
    13321 caccgctccg gcgtccgctc cccagggggt cgagagcccg ttcaccgctg agttcgccaa
    13381 gaccggcatc gacacgatct tctcgtcgta ctactgggac tgcaccaacc tgatggcgct
    13441 cgcggcggtc aaggccaaga gcgacagcgg tgaggcgatc gccgagaact tcgccgccaa
    13501 cctcgagggc gacaacgact gcaacaactt caaggactgc aaggcgctcc tcgaagaggg
    13561 caagtcgatc cactaccgcg gcgcctcctc ggcgttcgag aagtggaaca agatggagcc
    13621 cggcaccggc gtctacgacg tctgggcgta caccgccgaa ggtaaggacg ccaacgtcga
    13681 gggcgcggcc cagatcaaga tcggctgatc gggccggcgg acggctccgg ccgtacgact
    13741 ctcctgcggg gcgcccttcc gggggcgccc cgcagcgcgt ccggggtcgg gtcagcgtcc
    13801 ggggttcggg tcgagttggg ccggatcggc tcagcggttg cggaccaacg agcccaggta
    13861 cagctcgatg accttggggt cctcgaggag ttctgccccg gtgccggtgt aggcgtcggt
    13921 gccgttgtcg agcacgtagc cccgatggca gatccgcagc gccttgtggg cgttctgctc
    13981 gacgatgagc accgacacgc ccgcgtggtt gatcgcggcg atgcgggcga acacctcgtc
    14041 ctgcatggcg ggggacaggc cggcggacgg ctcgtcgagc aggaggaggc tgggctccat
    14101 catcagcgcc cgggccatgg cgagcacctg gcgttgcccg ccggagaggg cgtcggcccg
    14161 atccccgcgt cggtccctca gcgtggggaa cagatcgagc acgtcgtcgc gtcgagcggc
    14221 gaagcggtcc gggcgcaggt acacgcccat gcggaggttc tcctcgacgc tcagggtgcc
    14281 gaacacgttg cggacctggg ggacgtagcc gaccccgcgc gacaccaggt cgtagccgtg
    14341 gacgccgacg atgtcgtcgc cgtcgagggt catcgtcccg gaccggaccg gcaccaggcc
    14401 gaacacggcc ttgatcaggg tggacttccc ggcgccgttg ggtccgatga tgccgaccag
    14461 ctcggatcgg gcgacgtcga tcgaggtccc ccggaggatg tcgacgccgg cgacgtagcc
    14521 ggcgacgagg tcacggacga cgagcagcgg ttcgccggtc atggccgtgc tccgtcggaa
    14581 tctgtgccgt cggaatctgt gccgtcggaa tctgtgctgt cgggagtcct gccgtcggcg
    14641 tagagctcgt cgacgacggg ggcgagctcg tcggccatgt cgcgcaggtc accgctcgat
    14701 ccgcccaggt aggcgtcgat cacccgcgga tccgacgcga cctgcccggc ggtccccgag
    14761 gcgatgatgc ggccctcggc catgcacacc accacgtcgg agatggccga gaccacgtcc
    14821 atgtcgtgct cgacgaacag gacggtgatg ccctcgccgg gcagctcgcg gatcttgtcg
    14881 agcagcagct g
//
LOCUS       DAOQOD010000072        14696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1448_length_14696_cov_11.310361, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000072 DAOQOD010000000
VERSION     DAOQOD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_14696_cov_11.310361"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PLZ72_08620"
     CDS             <1..1352
                     /locus_tag="PLZ72_08620"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNA36397.1"
                     /translation="RCAAARQGWIDQVAATRPDVVVVMAGTRDLADRRLSPSTPWAAP
                     GSPEIDDFLRADVADLLGDLAATGTQVVVLSTPPVRSTAPDPAPVPTTLPADPTQAAM
                     LVAEGQEAAAGVPAAGHAENDDARRAAWNSVLRSAASSAGVTFLDADAEMATWPGGAL
                     DPRWRADGIGLSPEGAMRLSRWIAPRIRDLQTGVAAPAPLSALSTAGELPPAPPPTPR
                     RTVPAGRAVDTLVVGDSVAHGIGYGLERWSKGRSDIEVLNGARLGCPIARGGSFRFRR
                     DIATFGADCDWATVFPGLLQGQRPDVIVLGTGIWEVVDRRFPGDDRWRHIGQPEVDAY
                     ILKELVSAIDVLGASGAKVVLLTYPHFQAGLDQGFTNLPESDPARVDRLNELLRQAAA
                     ARPGVATVVDLQGWYAQQPPDQDRVRRPDGLHFTDEFAPTIGGWLGPQLVALGRTPI"
     gene            complement(1408..2979)
                     /locus_tag="PLZ72_08625"
     CDS             complement(1408..2979)
                     /locus_tag="PLZ72_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA36398.1"
                     /translation="MTDAPAQDDSPTPDDSPDVVVIGAGPAGLTAAYQLSKDGVAATI
                     LEADDIVGGISRTAERDGWRFDIGGHRFFTKVAPVEAVWHEILSDDEFLKRPRLSRIY
                     YQGKYYDYPIKPLNALRNLGFIEAVRCTLSYLWAKVRPPKDQSTLEGYVVADYGRRLY
                     EHFFRTYNIKLWNVDPKYLSSDFGAQRIKGMSLWSAVWEPIRTRLFGSRADRAKQVTS
                     LIEEFQYPKYGPGMMWERCAEIVTERGAELHMETTVTTVRRDPDAKRATAVVARAADG
                     SAVEHAADEVISTMPFSHLLKAMDPPPPAEVVAAADQLRFRDFLTIALVVPVEYGFPD
                     NWIYIHAPEVQVGRIQNFGQWSPYLVKDGRTCLGLEYFVFEGEGLWSKPDDELVALGT
                     QELAALGLVDPSRVEAGYVVRMPKAYPVYDEDYKANVAVLRRWLEANVPNVHPVGRNG
                     MHKYNNQDHSMLTAMLTVENILGRGDHDIWSVNVEEEYHEEGSGVDTAPASATSTAGT
                     GRDAPVVPSSAPRVN"
     gene            complement(3122..3391)
                     /locus_tag="PLZ72_08630"
     CDS             complement(3122..3391)
                     /locus_tag="PLZ72_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36399.1"
                     /translation="MTSLLTDPDRSRTREVTFDVTLRDGTVQRVDGADAYEPERSMTT
                     FFRTDGRPTVDCWSVRMASFRTDEILAIRRLEDVEVGAEPALRIV"
     gene            complement(3554..5260)
                     /locus_tag="PLZ72_08635"
     CDS             complement(3554..5260)
                     /locus_tag="PLZ72_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA36400.1"
                     /translation="MSTVELNVAVITEAIAAAHPDRDGLVFRDRRLSWADVADRTRRL
                     ARVLLDSGIGLRSEFGSGPRWESVHDHVALYLHNGNEYLEGMLGAWKARAAPFNVNYR
                     YVADELRYLLDDASARAVIVHECFAPTLAAVLDSLADPPALILQVADGSGHPLVPGAV
                     DYDAALAAVGPTTSPVELPDDVVSGWSGDDLYLCYTGGTTGMPKGAMWRQEDFLIAAL
                     GVRRRDGTDFEDVEEIVAAAASGRLRALPAPPLMHGAAHWNALSCWIAGGTVIIQDHP
                     EHLDPEDLLRTVERHRATSLLVVGDPVARPLADALAAARADGRAHDLSSLRHLLSGGA
                     VLSPALKAELVELVPGLTVVDVLGSTESGRQGVSNVRAGDAPSVGFVPSSTSVVLDES
                     RIARLAPGDPAIGWLAQSGRVPLGYLNDEEKTAATFPEIDGVRHAVAGDRARLRADGT
                     IELHGRDSVCINTGGEKVFAEEVETALKTHPDVFDAVVCGRPSPRWGEEVVALVQMRS
                     GSDADDEGLRAAAGEHLARYKLPKEIIRLDRLVRSPSGKADYRWARAVAADPPRAEGS
                     AP"
     gene            5310..5948
                     /locus_tag="PLZ72_08640"
     CDS             5310..5948
                     /locus_tag="PLZ72_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36401.1"
                     /translation="MPSPATRPAWSAAAARLAERDPVMAALHDRHGAPRLARGPRADA
                     RFEALANSIAYQQLNGRAAATIWGRVLDAVGPSFTPEAVLAVDPSQLRAAGLSNAKTA
                     AILDLAARVDDGRVRLARIGSLDDDEVIEHLVTVRGIGPWTAQMFLMFDLRRIDVWPT
                     GDYGVRAGFARAHGWEAIPTEKEMVALGEPFSPYRSVVAWWCWREVDTATPG"
     gene            5981..7441
                     /locus_tag="PLZ72_08645"
     CDS             5981..7441
                     /locus_tag="PLZ72_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA36402.1"
                     /translation="MERTDRDRQRADDLWLAERLRATDHLDCLREEDFSRLDRDGHVY
                     LDHTGAGLYPESLVRDHLEMLAGGVFGNPHSDNPTSRPASEMVEEAREAVLRFVGADP
                     DTYECIFTPNATGAIRLVAEAFPFGADRPLVLSSDNHNSVNGIREHAHRAGAPVTYVP
                     VLAPDLRLDQERLLSALEGPPGLLALPAQSNYSGVQHPLPEVPDGWRVLWDAAAFAPT
                     NPLHLDDRHVDFVSLSFYKVFGYPTGVGALIARRDALAELVRPWFAGGTIAIASVAAA
                     GHRLVPGHAGFEDGTLDFLSLPAVRAGIEVMERIDINSVHDRVQVLTAWTLERMQDLR
                     HANGSPLVQVLGPWEPVDRGATVAFVLVDPDGHELADRSVERMATTAGISLRTGCFCN
                     PGAGETARDVSADDLRPFLAADTYEGRPVSLCEVDQVFRRHRDVGVSALRVSLGMSSN
                     EADVRTWIDFLGGLLDRRVDELDPPPPVPPPRPDGA"
     gene            complement(7482..8501)
                     /locus_tag="PLZ72_08650"
     CDS             complement(7482..8501)
                     /locus_tag="PLZ72_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36403.1"
                     /translation="MAGSGSAHLRVDQDVLLRVENLVVDFPAGRGRKVSAVANVSFDV
                     AQGETLGLVGESGCGKSTTGKAVMQLPRPTRGSVRLDGTELTALKGAELRQVRPRMQM
                     IFQDPISSLNPRRTVSQIVGEPLSIWKTGDAASRTAKVEEVLTAVGVDPAAAAERKPH
                     EFSGGQCQRISIARAVITDPQLIICDEPVSALDVSVQAQILNLLEDMKASYGLTLVFV
                     AHDLAVVKNVSDRIAVMYLGKMCEIGPPDALFDRPAHPYTDALVAAIPLPDPTVDTST
                     LPHLGGELPSPLDPPSGCRFRTRCPRAQELCASAEPQMQKIAEGHYVACHFPLETPVA
                     EPGVH"
     gene            complement(8501..9697)
                     /locus_tag="PLZ72_08655"
     CDS             complement(8501..9697)
                     /locus_tag="PLZ72_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36404.1"
                     /translation="MSGPEVIDGPTQSGLPGSGPLLEVADVATSFRSPRGLVRAVDGV
                     SFTLERGQCLGIVGESGSGKSVLSRSIMGLLPSTAERSGRVTFEGRDISELSSEGYRE
                     LWGTEMAMVFQDPMTALNPVMKVGNQITESLRYHLDMDKSEARETAVALLRAVRIPEA
                     DKRFTSYPHEMSGGMRQRVVIAVALACGPKLLFADEPTTALDVTVQAQILDLLGEQQR
                     ERYMAMILVTHDLGVVASRAQEIAVMYAGRVVEKAPTAALFADMQMPYTEALLAAIPK
                     LGDGSHTRLSAIGGRPPDLVNPPKGCAFSPRCPYARERCHEESPTLQENPNVPGHQFA
                     CFYPVSSPETLEIKDRLAAQKMMAATPPPGAPAVDPAVSNTPPPFSGPAPATPPPFPG
                     PSQGTL"
     gene            complement(9694..10662)
                     /locus_tag="PLZ72_08660"
     CDS             complement(9694..10662)
                     /locus_tag="PLZ72_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA36405.1"
                     /translation="MPAPDQVPVHDPSLVNLEPSLSVYGLAVESDQLEPVPEGGRPRR
                     KRLGVGAWLAIVWLGGLLALAILAPLLPIPDPNQSFTEIARKGPTAGHWLGGDANGRD
                     LLARLIYGTRASFAIGFGAVGFGLVIGGLLGLATGFFRGKVDGIITPIMTILLAIPQF
                     VLALSLVTVLSSGDQVSSVQRIGVVILGLGIVSVPILGRITRANTLSWSEREFVLAAR
                     AMGAKNWRIMLRDILPNVFPAMLSISLLGVGIAIVAEGGLALFGVGVQPPTPSWGNII
                     AENKDQLNRAPHIVMFTIVVLFLTVLALNFLADVVGKRFEVRESLL"
     gene            complement(10649..11596)
                     /locus_tag="PLZ72_08665"
     CDS             complement(10649..11596)
                     /locus_tag="PLZ72_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA36406.1"
                     /translation="MGIARKLLQLVITVILVTLFASFLLELLPGDPVEVLVPFGSDEQ
                     RQLVREETGLDQPFLVRYGNWLGGFVTGDMGNQYTVSSTRPVVDRVTTSLWPTVLLMV
                     YAQTLALIIAIPVGVIAAYRQDTWFDRLSNTSAFAMLAIPTFALGFVLQYYIGVKLGW
                     ADTGGYKSPTQDLAGHVRSMILPTITLAVGQIAVYMRLLRSDMIATLQQDFITMAKAK
                     GITTKRILFRHALRPSSLTLLTVAGLNIGTLIGGALIIEVVFNIPGLGYLMGEAIATR
                     QIVMFQSLVAIIAIFYVFVNFCVDILYTVLDPRIRHAGT"
     gene            complement(11725..13326)
                     /locus_tag="PLZ72_08670"
     CDS             complement(11725..13326)
                     /locus_tag="PLZ72_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36407.1"
                     /translation="MVAAACGGSDNSSGGGGSGNDADAGKPVSGGSVTYGLEAETAGG
                     WCLPEAQLAIAGIQVARTMYDTLTAPDENGDYVPFLAQSVEPNADYTQWTIKLREGVK
                     FHDGTTLDSTVVKNNIDAFRGQYPTRKSLLFVFVLSNIASVDVVDPLTLTVTTKTPWP
                     AFPAFLHSSGRLGISAQAQLDDPATCDRNLIGTGPFKLKEWKLNDHLTAVKNPDYWQK
                     DADGNQLPYLDEISYRPIPDSDARVNALLAGELTAMHTSLPESIDRLRTEKENGKVGL
                     VESDKYAEVAYIMLNASKAPFDNINARMAAAYSLDRDSFNQVRNLGLTKVASGPFAEG
                     SVGYLPDAGFPKYDLDKAKDYAAKYTEETGKPLEITVLSTPDPATVKSAQFIQEQVEK
                     AGFKVNLKTVEQAALINNALGSDWNAMAWRNHPGGAPDLQYVWWNSAAPTNFGKFKDP
                     EMDQLLNEGRAESDKAAAKTIYENVNKRFGSQAWNLWLNWTIWDIASAPDVHGVYGPD
                     FPGGEKPFPGLATGHPVSGMWVAKG"
     gene            complement(13550..>14696)
                     /locus_tag="PLZ72_08675"
     CDS             complement(13550..>14696)
                     /locus_tag="PLZ72_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNA36408.1"
                     /translation="APPAPGVPGLTPEVAALPASSRDLDRVRSARDAAVATLDRDRRE
                     LDGTRISRETAQQQQQAEATLLARRRQERDKVDAVLTRQRSALRAVASEWYMTGNADR
                     RAIDPTLGASELAELRRQAVLGDAAAAATADAIAFLDRRRHGLSEEIDALERSSERVA
                     GRLEELTRREQELSTAVAADEVAVSGAETAVAAARLNATVDGTDISTVALDAYWRAQG
                     HMAVTDPGCRISWWALAGIGRTESRHGTYLGSAVGVDGRAEPPIVGPPLDGTNGFRKV
                     PDSDGGALDGDAGSDRAVGPMQFLPGTWRTVGRDGNGDGRADPDNIYDAALGAAVYLC
                     RSGDLSAEPALRGAYLTYNRSLVYVDTVIGFATAYRDAVALPSPAAR"
BASE COUNT         2112 a         5026 c         5367 g         2191 t
ORIGIN      
        1 accgctgcgc ggcggcccgc caggggtgga tcgaccaggt cgccgccacc cgccccgacg
       61 tcgtggtggt catggccggg acccgcgacc tggcggaccg gcggctgtcg ccctcgacgc
      121 cgtgggccgc gcccggatca ccggagatcg acgacttcct gcgagccgac gtggcggacc
      181 tgctcggcga cctggcggcg accggcacgc aggtcgtcgt cctgtcgaca cctccggtcc
      241 gcagcacggc gcccgatccg gcaccggtcc cgaccacgct gcccgccgat ccgacgcagg
      301 cggcgatgct cgtcgccgag ggccaggagg ccgccgccgg tgtccctgcg gccggtcacg
      361 ccgagaacga cgacgcccgg cgggcggcat ggaactcggt gctgaggtcg gcggcctcct
      421 cggccggtgt gaccttcctc gacgcggatg ccgagatggc gacctggccc ggcggtgccc
      481 tcgacccacg gtggcgcgcc gacgggatcg gtctcagccc cgagggtgcc atgcggctgt
      541 cacgctggat cgcgccccgc atccgggacc tgcagaccgg cgtcgccgcg ccggctccgc
      601 tctccgcgct gtcgaccgcg ggggagctcc cgccggcgcc tccgccgacc ccgcgacgga
      661 ccgtcccggc cggccgggcg gtcgacacgt tggtggtcgg cgactcggtc gcgcacggga
      721 tcggctacgg cctggagcgg tggtccaagg ggcgctccga catcgaggtg ctgaacggcg
      781 cccggctggg gtgcccgatc gcccggggcg gcagcttccg gttccgccgc gacatcgcga
      841 cgttcggggc ggactgcgac tgggcgacgg ttttcccggg gctgctgcag ggccagcggc
      901 ccgacgtgat cgttctgggc accggcatct gggaggtggt cgaccgccgg ttccccggag
      961 acgaccgctg gcgccacatc ggccagcccg aggtcgacgc ctacatcctc aaggagctgg
     1021 tgtcggcgat cgacgtgctc ggtgcgtccg gcgccaaggt cgtgctgctg acctacccgc
     1081 acttccaggc cggcctcgac caggggttca cgaacctgcc cgagtcggat ccggcccggg
     1141 tcgaccgcct caacgagctg ctccgccagg ccgcggccgc ccgcccgggg gtggcgacgg
     1201 tggtcgacct ccagggctgg tacgcccagc agccgccgga ccaggatcgg gtgcgtcgac
     1261 ccgacgggtt gcacttcacc gacgagttcg ccccgacgat cggcgggtgg ctcggcccgc
     1321 aactcgtcgc cctcggccgc accccgatat aggggtcctc accgacacac cttgtcgttc
     1381 tgcgcgtacg agaaaccccg acggggctca gttcactcgc ggggcgctgc tcgggacgac
     1441 cggggcgtcg cggccggtgc cggccgtcga ggtggccgag gccggggcgg tgtccacccc
     1501 ggagccctcc tcgtggtact cctcctcgac gttgaccgac cagatgtcgt ggtcgccccg
     1561 cccgaggatg ttctcgacgg tcaacatggc ggtgagcatc gagtggtcct ggttgttgta
     1621 cttgtgcatg ccgttgcgcc cgacgggatg cacgttgggg acgttggcct cgagccagcg
     1681 gcgcagcacc gcgacgttgg ccttgtagtc ctcgtcgtag accgggtacg ccttgggcat
     1741 gcgaacgacg tagccggcct cgactcggct cgggtcgacc aggcccagcg cggcgagctc
     1801 ctgggtgccg agggcgacga gctcgtcgtc cggcttggac cacaggccct cgccctcgaa
     1861 cacgaagtac tcgaggccca ggcacgtgcg cccgtccttg accaggtacg gcgaccactg
     1921 gccgaagttc tggatgcggc cgacctgcac ctcgggggcg tggatgtaga tccagttgtc
     1981 ggggaagccg tactcgaccg ggacgaccag cgcgatcgtg aggaaatcac ggaagcgcag
     2041 ctggtccgct gccgcgacga cctcggccgg cggcggcggg tccatggcct tcaacaggtg
     2101 cgagaagggc atggtcgaga tgacctcgtc ggcggcgtgc tccacggccg agccgtcggc
     2161 ggcccgggcg accaccgcgg tggcccgctt ggcgtccggg tcccgccgga cggtggtgac
     2221 ggtggtctcc atgtggagct cggcgcctcg ctcggtgacg atctcggcgc agcgctccca
     2281 catcattccg gggccgtact tggggtactg gaactcctcg atcaggcttg tgacctgctt
     2341 ggcgcggtcg gcgcggctgc cgaacagccg cgtccggatc ggttcccaca ccgcgctcca
     2401 cagcgacatt cccttgatgc gctgggcgcc gaagtcgctg gagaggtact tggggtcgac
     2461 gttccagagc ttgatgttgt aggtccggaa gaagtgctcg tagagccggc gtccgtagtc
     2521 ggcgacgacg tagccctcga gggtgctctg gtccttgggc ggccggacct tggcccagag
     2581 gtacgacagg gtgcagcgga cggcctcgat gaagccgagg ttgcgcaggg cgttgagcgg
     2641 cttgatcggg tagtcgtagt acttgccctg gtagtagatg cggctgaggc gggggcgctt
     2701 gaggaactcg tcgtcgctga ggatctcgtg ccagacggcc tcgaccgggg cgaccttggt
     2761 gaagaaccgg tgaccgccga tgtcgaagcg ccacccgtcg cgttccgcgg tgcggctgat
     2821 cccgccgacg atgtcgtcgg cctcgaggat ggtggcggcc acgccgtcct tggacagctg
     2881 gtaggcggcg gtcagcccgg ccgggcccgc accgatgacg acgacgtcgg gggagtcgtc
     2941 cggggtgggg gagtcgtcct gggcgggtgc gtcagtcatg ggagatcgtc ggggtcctgg
     3001 agagcggcgg ggacgggagg cggcgggatc cggcacggcc cgcacgtccc ccaaacgtag
     3061 ccgccagccc ctggcgagcc cgaacatgca cgttgcgacc gcgtccgcgc tgcgccgggc
     3121 gtcacacgat gcgcaaagcg ggctcggcgc cgacctccac gtcctcgagg cggcggatcg
     3181 ccaggatctc gtcggtgcgg aagctggcca tcctcacgct ccagcagtcg accgtcggcc
     3241 ggccgtcggt gcggaagaac gtcgtcatcg accgctcggg ctcgtaggcg tcggccccgt
     3301 cgacgcgctg cacggtgccg tcgcgcagcg tgacgtcgaa ggtgacctcc cgggtgcggg
     3361 agcggtccgg atcggtgagc agcgaggtca tgggctcacg ctacgaaggc gaccctgtgg
     3421 atcgatagac cgcgaaccgt gtgatcttcc cgtgcaccgc ctgtgtcccc gttgtggtcc
     3481 agctgtgtgc aaccccactc ccacagggga tcacagtgga tcgggcgagt ctgggccgga
     3541 tcacgaccct ggttcagggg gccgagccct ccgcccgggg cgggtcggcc gccaccgccc
     3601 gggcccaccg gtagtcggcc ttgcccgacg gcgagcgcac cagtcggtcg agccggatga
     3661 tctccttggg cagcttgtag cgggccaggt gctcgcccgc cgcggcccgc agcccctcgt
     3721 cgtcggcgtc ggagccggag cgcatctgca ccagcgccac gacctcctca ccccagcgcg
     3781 gcgacggacg gccgcacacg accgcgtcga acacgtcggg gtgggtcttc agggcggtct
     3841 cgacctcttc ggcgaagacc ttctccccac cggtgttgat gcacaccgag tcgcggccgt
     3901 gcagctcgat ggtgccgtcg gcccggaggc gggcccggtc gccggccacc gcgtggcgga
     3961 ccccgtcgat ctcggggaac gtcgccgcgg tcttctcctc gtcgttcagg tagccgaggg
     4021 ggacccggcc ggactgggcc agccaaccga tcgccggatc gcccggcgcc aaccgggcga
     4081 tccggctctc gtcgaggacc accgacgtgc tcgacggcac gaaccccacg gacggagcgt
     4141 ccccggcgcg gacgttggag accccctggc gacccgactc ggtggagccc aacacgtcga
     4201 cgaccgtgag ccccgggacc agctccacca gctcggcctt cagcgcgggc gacaggaccg
     4261 caccacccga caggaggtgc cgcaacgacg acaggtcgtg ggctcgaccg tcggcgcggg
     4321 ccgcggccag ggcatcggcc aacgggcgcg ccaccgggtc gccgaccacc agcagcgacg
     4381 tcgcacggtg gcgctcgacc gtgcgcagca ggtcctcggg atcgaggtgc tcgggatggt
     4441 cctggatgat gacggtccca ccggcgatcc agcatgacag ggcgttccag tgcgcggcgc
     4501 cgtgcatcag cggaggggcc ggcagggccc ggagccgacc cgacgctgcg gcggcgacga
     4561 tctcctcgac gtcctcgaag tcggtgccgt cccgacgccg gacgccgagc gcggcgatca
     4621 ggaagtcctc ctggcgccac atcgccccct tgggcatgcc ggtggtgccg ccggtgtagc
     4681 agaggtacag gtcgtcgccg gaccagccgg agaccacatc gtcgggaagc tcgaccggcg
     4741 aggtggtcgg accgaccgcg gcgagcgccg cgtcgtagtc gacggcgccg gggacgagcg
     4801 ggtgaccgct gccgtcggcg acctgcagga tcagcgccgg tggatcggcc agcgagtcca
     4861 acaccgcggc cagcgtcgga gcgaagcact cgtggacgat caccgcccgg gccgaggcgt
     4921 cgtcgagcag gtagcgcagc tcatcggcga cgtagcggta gttgacgttg aacggagccg
     4981 cccgcgcctt ccatgctccg agcatcccct cgaggtactc gttgccgttg tggaggtaca
     5041 gggcgacgtg gtcgtgcacc gactcccagc gcggacccga tccgaactcg gagcggagcc
     5101 cgatgccgga gtccagcagc acgcgggcca gtcggcgggt gcggtcagcg acgtcggccc
     5161 agctcagtcg tcggtcccgg aacaccaggc cgtcgcggtc gggatgggcg gcggcgatcg
     5221 cctcggtgat cacagcgacg ttcagctcca cggtcgacat gctggcagga tggcatggtg
     5281 atcggcacct cccgctcccg caggccctga tgccgagccc ggccacccgc cctgcgtgga
     5341 gcgccgcggc ggcgcggttg gccgagcggg acccggtcat ggcggcgctg cacgatcgcc
     5401 acggcgcccc ccgcctggcc cggggcccgc gggccgacgc ccgcttcgag gccctggcca
     5461 actcgatcgc ctaccagcag ctcaacggtc gggccgcagc gacgatctgg ggtcgggtgc
     5521 tcgacgccgt cgggccgtcc ttcacccccg aggccgtcct ggcggtcgac ccgtcgcagc
     5581 tgcgggccgc cggtctgtcg aacgccaaga ccgcagcgat cctcgatctg gccgcccggg
     5641 tcgacgacgg tcgggttcgc ctcgcccgga tcggatcgct cgacgacgac gaggtgatcg
     5701 agcacctggt caccgtgcgg gggatcggtc cgtggaccgc gcagatgttc ctgatgttcg
     5761 acctgcgccg gatcgacgtg tggccgacgg gtgactacgg cgtgagggcc ggcttcgccc
     5821 gggcccacgg atgggaggcg atacccaccg agaaggagat ggtcgccctc ggcgaaccct
     5881 tctcccccta ccgcagcgtg gtcgcctggt ggtgctggcg ggaggtcgac accgccactc
     5941 ccgggtgacc ccgagcggtg tccgcggcca gactgggccg atggagcgga ccgaccggga
     6001 ccgtcagcgc gccgatgacc tgtggctcgc ggagcggttg cgcgcgaccg atcacctgga
     6061 ctgtctgagg gaggaggact tctcgcgcct cgaccgcgac ggccacgtct acctggacca
     6121 caccggcgcc gggctctatc ccgagtcgct cgtgcgcgac cacctggaga tgctcgccgg
     6181 cggggtgttc ggcaaccccc actccgacaa cccgacctcc cgtccggcca gcgagatggt
     6241 cgaagaggcc cgcgaggcgg tgctgcgttt cgtcggtgcc gatcctgaca cctacgagtg
     6301 catcttcacc ccgaacgcga ccggtgccat ccgcctggtc gccgaggcgt tcccgttcgg
     6361 cgccgaccga cccctggtgc tcagctccga caaccacaac tcggtcaacg ggatccgcga
     6421 gcacgcgcac cgggccggag cgccggtcac ctacgtgccg gtcctcgccc ccgacctgcg
     6481 actcgaccag gagcgactgc tgagcgccct cgagggcccg cccggcctgc tcgcgctgcc
     6541 cgcccagtcc aactactccg gggtgcagca cccgctgccg gaggtcccgg acggctggcg
     6601 ggtgctgtgg gacgcagcgg cgttcgctcc gaccaacccg ctgcacctcg acgatcgcca
     6661 cgtcgacttc gtctcgctgt cgttctacaa ggtgttcggg tacccgaccg gggtgggggc
     6721 gctgatcgcc cgccgcgacg ccctggccga gctcgtccgg ccgtggttcg ccgggggcac
     6781 gatcgcgatc gcctcggtgg ccgccgccgg ccaccgactg gttccgggcc acgccggctt
     6841 cgaggacggc accctcgact tcctgtcgct gccggcggtg cgggccggga tcgaggtgat
     6901 ggagcgcatc gacatcaaca gcgtgcacga ccgggtgcag gtgctcacgg cgtggacgct
     6961 ggaacggatg caggatctgc gccatgccaa cggctcgccg ctggtgcagg tcctcgggcc
     7021 gtgggagccg gtggaccggg gggcaacggt ggcgttcgtg ctggtcgacc ccgacggcca
     7081 cgagctcgcc gaccggtccg tcgaacggat ggccacgacg gcggggatct cacttcggac
     7141 cggctgcttc tgcaaccccg gcgccggcga gaccgcgcgt gacgtgagcg ccgatgacct
     7201 ccggccgttc ctggccgccg acacctacga gggccggccg gtgtcgctgt gcgaggtcga
     7261 ccaggtgttc cgtcggcacc gtgacgtcgg cgtcagcgcc ctgagggtgt cgctcgggat
     7321 gtcgtcgaac gaggccgacg tccggacctg gatcgacttc ctgggcggac tgctcgaccg
     7381 acgggtggac gagctggatc cgcctccacc ggtcccaccc cctcgtcccg acggcgcctg
     7441 aggtcgcggc tcagctcacc tcgggtcagt gcagctacag atcagtgcac gcctggttcg
     7501 gcgaccggcg tctcgagcgg gaagtggcac gccacgtagt gaccctcggc gatcttctgc
     7561 atctgcggct cggccgacgc gcacagctcc tgggcccggg ggcagcgggt gcggaagcgg
     7621 caccccgacg gcggatcgag cggagagggg agctctccgc ccaggtgcgg gagtgtgctg
     7681 gtgtccaccg tcgggtccgg cagcgggatg gccgcgacga gggcgtcggt gtacgggtgg
     7741 gcgggccggt cgaacagcgc gtcgggaggc ccgatctcgc acatcttgcc caggtacatg
     7801 accgcgatgc ggtccgagac gttcttgacg accgccaggt cgtgggcgac gaacacgagg
     7861 gtcaggccgt agctggcctt catgtcctcg agcaggttga ggatctgggc ctggaccgac
     7921 acgtccagag cggagacggg ttcgtcgcag atgatcagct gcgggtcggt gatgaccgcg
     7981 cgggcgatcg agatgcgctg gcactggccg ccggagaact cgtggggctt gcgctcggcc
     8041 gcggctgcag gatcgacgcc gaccgcggtc aggacctcct cgaccttggc ggtccgggag
     8101 gcggcgtcgc cggtcttcca gatcgacagc ggttccccga cgatctggct gaccgtgcgg
     8161 cgggggttca gcgagctgat cgggtcctgg aagatcatct gcattcgggg ccggacctgg
     8221 cgcagctcag cacccttgag ggcggtgagc tccgtgccgt cgaggcgcac cgacccacgg
     8281 gtggggcggg ggagctgcat gacggccttg ccggtggtcg acttgccgca gccggactcg
     8341 ccgaccaggc cgagggtctc gccctgggcg acgtcgaagg acacgttggc gaccgcggag
     8401 accttgcggc cgcggccggc ggggaagtcg acgaccaggt tctcgacccg caggaggacg
     8461 tcctggtcga cgcggaggtg cgccgatccg ctgccggcca tcagagggtc ccttgcgacg
     8521 ggccggggaa cggcggagga gtggcgggcg ccgggccgct gaacggcggc ggggtattcg
     8581 agacggccgg atcgaccgcg ggagcacccg gcggaggggt cgccgccatc atcttctggg
     8641 cggccaggcg gtccttgatc tccaacgtct cgggggagct caccgggtag aagcacgcga
     8701 actggtggcc ggggacgttc gggttctcct gcagggtcgg gctctcctcg tggcaacgct
     8761 cgcgggcgta ggggcagcgg ggactgaacg cgcacccctt cggtgggttg accaggtccg
     8821 gcgggcgtcc gccgatggcc gacaaccggg tgtgggaccc gtcgccgagc ttggggatgg
     8881 ccgcgagcag cgcctcggtg tagggcatct gcatgtcggc gaagagagcc gcggtgggcg
     8941 ctttctcgac gacgcgaccg gcgtacatga cggcgatctc ctgggcccgc gacgccacca
     9001 ccccgaggtc gtgggtgacc aggatcatgg ccatgtaccg ctcgcgctgc tgctcgccga
     9061 gcaggtccag gatctgggcc tgcaccgtca cgtccagggc ggtggtcggc tcgtccgcga
     9121 acaggagctt gggcccgcag gccagagcga cggcgatgac gacccgctga cgcatgccgc
     9181 cggacatctc gtgcgggtac gaggtgaagc gcttgtcggc ctcggggatg cgaaccgccc
     9241 gcaacagcgc cacggcggtc tcccgcgcct cggacttgtc catgtcgagg tggtagcgga
     9301 gcgactcggt gatctggttg cccaccttca tcaccgggtt cagcgcggtc atcgggtcct
     9361 ggaagaccat cgccatctcg gtgccccaca gctcgcggta cccctcggag ctcaactccg
     9421 agatgtcgcg accctcgaag gtgacgcggc ctgaacgctc ggctgtggag ggcagcaggc
     9481 ccatgatcga acgggacagc accgacttgc cggacccgga ctcgcccacg atccccaggc
     9541 actgaccccg ctcgagggtg aacgacacgc cgtcgacggc acgcaccagt ccgcgcggcg
     9601 agcggaacga cgtggcgacg tccgcgacct cgagcagggg tccggaaccg gggaggcccg
     9661 actgggtcgg gccgtcgatg acctcggggc cgctcacagc aggctctccc tcacctcgaa
     9721 gcgctttccg acgacgtcgg ccaggaagtt cagcgcgagc acggtcagga acagcacgac
     9781 gatggtgaac atgacgatgt gcggcgcccg gttcaactgg tccttgttct cggcgatgat
     9841 gttgccccac gacggggtcg gcggctggac cccgacgccg aagagggcga ggccgccctc
     9901 ggcgacgatg gcgatgccca cgccgagcag cgagatcgac agcatggcgg ggaacacgtt
     9961 gggcaggatg tcgcgcagca tgatccgcca gttcttggcc cccatcgccc gggcggccag
    10021 gacgaactcc cgctcggacc agctcagggt gttggcccgg gtgatgcggc cgaggatcgg
    10081 gaccgacacg atccccagac ccaggatcac gacgccgatc cgctgcacgc tgctgacctg
    10141 gtcgcccgag gacaggacgg tcaccagcga cagcgccagg acgaactggg ggatggcgag
    10201 caggatcgtc atgatcgggg tgatgatccc gtcgaccttg ccccggaaga agccggtggc
    10261 caggccgagc agaccgccga tgaccaggcc gaagcccacg gcgccgaagc cgatggcgaa
    10321 gctggcgcgg gtgccgtaga tcagccgggc caacaggtcg cggccgttgg catcgccgcc
    10381 gagccagtgc ccggcggtcg gacccttgcg ggcgatctcg gtgaaggact ggttggggtc
    10441 ggggatgggc aggagcgggg cgaggatcgc cagcgccaac agcccgccca gccagacgat
    10501 ggccagccac gcaccgaccc cgaggcgctt tcggcgcggg cgcccgccct cgggaaccgg
    10561 ttcgagctga tcggactcga ccgccagccc gtagaccgac agcgacggct cgaggttgac
    10621 gagcgacggg tcgtggacgg ggacctggtc aggtgccggc atgtcggatc cgggggtcga
    10681 ggacggtgta gaggatgtcg acgcagaagt tcacgaacac gtagaagatg gcgatgatcg
    10741 ccacgagcga ctggaacatg acgatctggc gtgtggcgat cgcctcaccc atcaggtagc
    10801 cgaggccggg gatgttgaac acgacctcga tgatcagagc cccaccgatc agggtgccga
    10861 tgttcaggcc ggccacggtc agcagcgtca gcgacgacgg tcgcagagcg tgtcggaaca
    10921 ggatccgctt ggtggtgatc cccttggcct tggccatggt gatgaagtcc tgctggagcg
    10981 tggcgatcat gtcgctgcgc agcagccgca tgtagaccgc gatctgaccg accgccaacg
    11041 tgatcgtcgg caggatcatc gagcggacgt ggccggccag gtcctgggtg ggtgacttgt
    11101 agccaccggt gtcggcccag cccaacttca cgccgatgta gtactgcagg acgaagccga
    11161 gcgcgaacgt cggtatggcc aacatcgcga acgcggacgt gttcgacaat cggtcgaacc
    11221 aggtgtcctg gcggtaggcg gcgatcacac cgacggggat ggcgatgatc agcgccagcg
    11281 tctgggcgta caccatgagc agcaccgtcg gccacagcga cgtcgtcacc cggtcgacca
    11341 cgggccgcgt ggagctcacc gtgtactggt tccccatgtc accggtgacg aatccgccca
    11401 gccagttgcc gtagcggacg aggaagggct gatcgagccc ggtctcctca cgaacgagct
    11461 gacgctgctc gtcgctgccg aaggggacga gcacctcgac cgggtcaccc ggcagcagct
    11521 ccaacaggaa cgacgcgaac aacgtcacca ggatcacggt gatgaccagt tggaggagct
    11581 tcctggcgat gcccactgat gcagacgtcc tgtcggtagg aatgggtggg aacgacgagc
    11641 ggagggtgga accgctccgg tcgggaccgg atgggggacg gggcgcccgg atggctccgg
    11701 gtcaccccgc ccacaccgac gggctcagcc cttggcgacc cacatcccgc tgacgggatg
    11761 accggtggcc aggcccggga acggcttctc gccgcccggg aagtcggggc cgtagacgcc
    11821 gtggacgtcg ggcgccgagg cgatgtccca gatggtccag ttgagccaca ggttccaggc
    11881 ctgcgaaccg aagcgcttgt tcacgttctc gtagatggtc ttggccgcgg ccttgtcgga
    11941 ctcggcccga ccctcgttga gcagctggtc catctccggg tccttgaact ttccgaagtt
    12001 ggtcggcgcc gcgctgttcc accagacgta ctggaggtcg ggagcgccac cggggtggtt
    12061 gcgccacgcc atggcgttcc agtccgaacc gagtgcgttg ttgatcagcg ccgcctgctc
    12121 gacggtcttg aggttcacct tgaagccggc cttctcgacc tgctcctgga tgaactgggc
    12181 ggacttgacc gtggccgggt ccggcgtgga cagcaccgtg atctcgagcg gcttgcccgt
    12241 ctcctcggtg tacttggccg cgtagtcctt ggccttgtcc aggtcgtact tggggaagcc
    12301 cgcgtcgggc aggtacccga ccgagccctc ggcgaacggc ccggacgcca ccttggtgag
    12361 gccgaggttc cggacctggt tgaacgagtc ccggtccagg gagtaagcgg ccgccatgcg
    12421 ggcgttgatg ttgtcgaagg gagccttcga cgcgttcaac atgatgtagg cgacctcggc
    12481 gtacttgtcg gactcgacca ggccgacctt gccgttctcc ttctcggtgc ggagccggtc
    12541 gatgctctcg ggcagcgacg tgtgcatggc ggtgagctca ccggccagca gggcgttgac
    12601 ccgggcgtcg ctgtcgggga tcggccggta ggagatctcg tcgaggtacg gcagctggtt
    12661 gccgtcggcg tccttctgcc agtagtcggg gttcttgacc gccgtcaggt ggtcgttgag
    12721 cttccactcc ttgagcttga acgggcccgt cccgatcagg ttccggtcgc aggtcgccgg
    12781 gtcgtcgagc tgtgcctggg cgctgatacc gaggcggccg gagctgtgca ggaacgccgg
    12841 gaacgcgggc cacggggtct tggtcgtgac cgtcaacgtc agcgggtcga ccacgtcgac
    12901 cgaggcgatg ttggacagga cgaagacgaa cagcagcgac ttgcgggtcg ggtactggcc
    12961 ccggaaggcg tcgatgttgt tcttgaccac ggtcgagtcg agcgtggtgc cgtcgtggaa
    13021 cttgacgccc tcacgcagct tgatcgtcca ctgcgtgtag tcggcgttcg gctcgaccga
    13081 ctgggccagg aacggcacgt agtcgccgtt ctcgtccggg gcggtgagcg tgtcgtacat
    13141 ggtgcgggct acctggatgc cggcgatcgc gagctgcgcc tcgggcaggc accagccgcc
    13201 ggccgtctcg gcctcgaggc cgtaggtgac gcttccgccg gacaccggct tgccggcatc
    13261 ggcgtcgttg ccggaaccgc ctccgccgga gctgttgtcg cttccgccac acgcggccgc
    13321 gaccatcgcg acggtgagaa cacctgccgc gagccacgac cagcgcgtgc tcttcgagcc
    13381 tcgtcgaacc accgttgata ccccctggga tgagcgaccc gcaccgtgtt gacggtcccg
    13441 ccgtgacggc gttcacaggt cgagtggggc gcactctagc gccgccgaac gcgctgtcaa
    13501 cgcacgtcgc gaccgatcat ccgttcacct tcgcccggca tcggacgcct catcgcgccg
    13561 cgggcgacgg gagcgcgacg gcgtcgcggt aggcggtggc gaagccgatg acggtgtcga
    13621 cgtagacgag gctgcggttg taggtgaggt aggcgccccg caacgccggc tcggccgaca
    13681 ggtcgccgct gcggcacagg tacaccgccg cgccgagcgc ggcgtcgtag atgttgtcgg
    13741 gatcggctct gccgtcaccg ttgccgtcgc gccccacggt tcgccaggtg cccggcagga
    13801 actgcatcgg acccacggcc cggtccgacc cggcatcacc gtcgagggcc cctccgtcgg
    13861 agtcgggcac cttgcggaac ccattggtgc cgtcgagcgg cggaccgacg atcggcggct
    13921 ccgcccgacc gtcgacgccg acggcgctcc ccaggtaggt gccgtggcgg ctctcggttc
    13981 gtccgatccc ggccaacgcc caccagctga tccgacagcc cgggtcggtc acggccatgt
    14041 gcccctgggc ccgccagtag gcgtcgagcg cgacggtgga gatgtcggtg ccgtcgacgg
    14101 tggcgttgag gcgagcggcg gcgacggcgg tctcggcgcc gctcaccgcc acctcgtcgg
    14161 ccgccaccgc ggtgctcagc tcctgctcgc gccgggtcag ctcctcgagg cgcccagcga
    14221 cgcgttcgct gcttcgctcc agagcgtcga tctcctcgga caggccgtgg cgtcgacggt
    14281 cgaggaacgc gatcgcgtcc gctgtcgcgg cggcagcggc gtcgcccagg acggcctggc
    14341 ggcgcagctc ggcgagctcg gatgcgccca gggtggggtc gatggcgcgc cgatcggcgt
    14401 tgccggtcat gtaccactcc gacgccacag cccgaagcgc cgatcgctgt cgggtgagca
    14461 ccgcgtcgac cttgtcgcgc tcctgtcgtc gacgggccag cagcgtggcc tcggcctgct
    14521 gctgctgctg cgctgtctcg cgtgagatcc gtgtcccgtc gagctcccgg cggtcgcggt
    14581 cgagggtggc caccgcggcg tcgcgggcgc tgcggacccg gtccaggtcc cgggagctgg
    14641 ccggcagggc cgccacctcg ggggtgaggc cggggacgcc cggtgccggc ggagcc
//
LOCUS       DAOQOD010000073        14605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1466_length_14605_cov_12.077182, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000073 DAOQOD010000000
VERSION     DAOQOD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_14605_cov_12.077182"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="PLZ72_08680"
     CDS             complement(<1..1744)
                     /locus_tag="PLZ72_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36409.1"
                     /translation="MTEEGRVPAASLAASAGVRDIPATSFVVPPRRGSLIDRDRMVKS
                     VLEPEAPILEVVAPPGFGKSTALVLCAEADDRPYAWVQLSEADDDAVHLGRHLALALD
                     RLAPLEPADGARIVGAGRSALGDIFPALAGVLADRSPCVVVLDDVHHLTSSEAEAGLQ
                     MLLSSVPEGSTVVLVGRRIPIALGRHRMDGRVHRVEASDLAMGLIEAAELFGRAGVSL
                     DDEELADLVTRVEGWPGGLHLAALALHQGRNPRSFSGADRLVAEYLIEEVLDGTEPEM
                     VSFMEGSAVLDRMAADELDELLERSDSSRMLGSIEGHGNLFLIPLDHDGQRFRYHHLF
                     RDHLLQRLRRDGPDRALELERRASGMRARSGDVDSAVRHAVRACDRERAAGLILGSVL
                     DRWLDGRLGQVGEWLDLLGTGAVDEIPEAAIAAAWCAVAAGDRDGAERSCRAAARFAD
                     HGPLSDGTPTVEVALAAVRMLMGSRGVDRVVSDAEVVRQAGGPGVNRLWALATGTQGS
                     GYAMLGRPDLARERLSEAIDAVGETPYVRAVGLALLALLALQDGELATAVTLSRSARS
                     TADAHGLECFAPAIS"
     gene            1939..2946
                     /locus_tag="PLZ72_08685"
     CDS             1939..2946
                     /locus_tag="PLZ72_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNA36410.1"
                     /translation="MQRSSDPGPADTVTFPGCPTPVAPLGVGTWAWGDSRTWGMGSYD
                     TALDETTIGDAWRSSLGAGVGLFDTAEVYGSGESERILGRLLSADPSGRDRMVIATKF
                     WPAPWRWNVVPALRQALEASLGRLQIPTVDLYQIHGPISLRSASAMAEALATVVDAGL
                     VRAVGVSNYSVREMTRIHSELRRRGVPLASNQIELSLLRTRPLSSGLIDACRDLGVVP
                     IAYSPIGQGRLTGKYSASNPPPGRRNFSAHPMEAVDRVVAVAREIGDAHDGRTPAQVA
                     LRWIIDKGAVPIPGAKNERQAVDNAGALGWSLDAAEVAALDRVALAGTDSIANRVWQH
                     G"
     gene            2994..4460
                     /locus_tag="PLZ72_08690"
     CDS             2994..4460
                     /locus_tag="PLZ72_08690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HNA36411.1"
                     /translation="MTEVIEAVTVVDGTGAPAFIGDVAIDEERIVEVVPREGAGSGLV
                     LAPGFIDSHTHDDLAVLVHPQMEFKVLGGVTTCVVGNCGMGAAPFPAAAAMGAVLHPG
                     HDLDPWDGYRGYLRRVDDTAPSVNVAALIGHGTVRSSVMGLDDGPPDDDQIAAMSRLI
                     DEGMAAGCVGMSTGLTYEPGIWARTEELTALAAVVAAAGGIYTSHIRDEGSHLIEAVD
                     EAIGIGRDAGLPVVVSHLKAMGEPNWGTVAAALERIDDAGPAVAADQYPYTASSTMLA
                     AVAASGRIGTLVTPDAVVIASTTDHPEWHGRSLAELADRLGVPGDRVATAVLDAEPVA
                     TVIVHSMSEDDVRTVLGHPGIMVGSDGIPSLEGHPHPRLYGTFARVLGRYVRDLGVLG
                     LEEAVHRMTGRPAEVFGLVDRGVIRPGAVADLVLFDPATVVDRGTFDDPRQSPDGIVG
                     VWVNGARVVTDGVHLGTRSGRALRRRRERVSRTGSASA"
     gene            complement(4742..6004)
                     /locus_tag="PLZ72_08695"
     CDS             complement(4742..6004)
                     /locus_tag="PLZ72_08695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA36412.1"
                     /translation="MSSTAESRDVLVIGGGPAGAAVAMRAAAGGARVTVFEKGRPGRD
                     KICGDGLTPRAVRALDALDVALDDAHRIDGLRMTANATVRQMDWPGIAPFPAHGAVWP
                     RRRLDERLMERAAAAGAELLWSHDAVPVVDEGVGAAPNRATGVTSVDADGRTRRWEAP
                     LVVLAAGAPGAAARLLGAERVPDETFGLAIRTYAESPRHAERYLEACLTLRDGSGTPV
                     PGYGWIFPAGDGTVNIGVGALSTMKGFKKLNLNALQDSYRALVGRDWELGPDLERPRA
                     WRLPMSARRRSGPGWVAIGDAAGLINPMNGEGIDYALESGTLAADLFLADPVTAPVRY
                     DAEVGERFDAFLRTGRRFSFLIGHPWILRNGLRVAVGTQAVAEITLQVMGNLVDGSTP
                     GAGGWTMRAADRVLAAGDPLLRRTRARP"
     gene            complement(6060..6893)
                     /locus_tag="PLZ72_08700"
     CDS             complement(6060..6893)
                     /locus_tag="PLZ72_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36413.1"
                     /translation="MDDRRRVPVPTPSGWTIESTRGFGPFVTRIRYRLADGTIRVWRS
                     RDHRKGLDRPGVPRRTWWIAVLFCIGSTCFTVGPLPAYESWVGTRWVGLTFFVGSIFF
                     TSASYLCFVEASNAPDAVVDDPQRRHRGPFRLISWRPHSIDWWSTGVQLVGTVYFNVM
                     TFLAMYDGWDTAQANRLVWRPDTIGSICFLVASYLAWAEVCHSAGRLRLRDVSWWIVV
                     LNLLGSVFFGVSAVGAYTLGSGELVNEWLDNAGTVAGGVCFFVAAAMLIPEARSKGAP
                     A"
     gene            7048..7680
                     /locus_tag="PLZ72_08705"
     CDS             7048..7680
                     /locus_tag="PLZ72_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA36414.1"
                     /translation="MGLYDDRVLPRLVDLTCGSKGMERYRAKAIDGLAGEVVEIGFGT
                     GLNVPLYPDAVTTVWAVEPSERSRELASDRVTARGVRVEHVGLDGQRLPLDDASCDAG
                     LCTFTLCTVPDPMRALAELRRVIRPGGQLHVLEHGAAPDPGIARWQERIEPWQKRLAG
                     GCHLTRDTTEMLRRAGFVELRLTTRFVKGPKPWSYFTWGRATNPRSPDVL"
     gene            complement(7750..>8150)
                     /locus_tag="PLZ72_08710"
     CDS             complement(7750..>8150)
                     /locus_tag="PLZ72_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNA36415.1"
                     /translation="VRFHRLVFPLREAVGSLMRREHDVVGPALQPYYQDLYDHVLRIT
                     ESTDSLRDLVATIVETNLSVRDYRQNQVMKKVTSWAAIIAVPTLVTGFYGMNVRFAGL
                     GTTWAAWLAVALIVVTSVTLYLMFRRRDWL"
     assembly_gap    8151..8250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8557..10104
                     /locus_tag="PLZ72_08715"
     CDS             8557..10104
                     /locus_tag="PLZ72_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36416.1"
                     /translation="MQQNDHGSDDQIPDVRTPTSPEALGLSRSLMEDLVIRRAVNDGR
                     SPMTALADKLHVSITVVESLLNSLRDRKLIEYDGMEGRAYVVRATEAGQQFATQRSRE
                     SIYCGPMPVPLDLYTAVVRHQRPQIELDHDRLQRSFSDLVLSDGLLDQLGPAIHGDGA
                     MFLYGPPGTGKSSVAERIIRAYEDVVLVPHCVEVDGQIVSVFDPTLHDEIDEQPPGMD
                     RRWVACHRPAVITGGELHAGMLDLQLERDSGVYLAPLQMKANNGILVIDDFGRQAMTP
                     DALLNRWIVPLDRSVDYLTLLGRKFEVPFELKVVLSTNLDPAKLGDEAFFRRIHNKIY
                     IGACTDEQFDWILSRVTRATGLELQAPAAVRLRQAARSQGDGELRAYLPGVVCKLANA
                     ICRYENQARVLTPELVDRVLALYFTEDLGVERTGPAPHHTVDVPPFAPDPELESEMAR
                     LAGPGPEQDSDAPLGAAPEAFDAPVDADGADAPVPPPYPGMFTGSLDPAAATIAEGLL
                     ADGLQPR"
     gene            complement(10149..10433)
                     /locus_tag="PLZ72_08720"
     CDS             complement(10149..10433)
                     /locus_tag="PLZ72_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36417.1"
                     /translation="MVTLLIEHEITDLATWTAAFERFADARRHAGVRSHRIAHPLDDP
                     TRIVVELDFDGAERAAEFRDFLREVVWADPTSSPALVGRPVTRLLESVPI"
     gene            10537..11541
                     /locus_tag="PLZ72_08725"
     CDS             10537..11541
                     /locus_tag="PLZ72_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA36418.1"
                     /translation="MSIEGLCATVRSERDLRIALLDELRRSVPFDAYAWVLTDPRTEV
                     GASPVADVPCLSELPRLIRLRYATEHARWTGAGDPVVTLVDSTAGRPEQSRLYRELLA
                     DLGVSDVASVVFRDVNGCWAFLDLWRMDGTFDPAETRLLRSGVEPITAALRRCVLHTF
                     ADTDPHVGRSLPTPVVILLDDDLRVREQTPTAEGYLATLLPPEADQRAIPAGAYNVAA
                     QLLAVEDGVDDHEPAARVHVEPLDWLVFRAARLGDDIAVTIERASPFERLELFARAAG
                     LSPRESELVEHLTTGADTRQVAALMFVSENTVQDHLKAVFAKTGVRSRRELLARVAGR
                     "
     gene            complement(11544..12392)
                     /locus_tag="PLZ72_08730"
     CDS             complement(11544..12392)
                     /locus_tag="PLZ72_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNA36419.1"
                     /translation="MYTPTSSHRTLRRGIAAGFALLALAGVLTACGGDDVTADKDRST
                     TTTTAKPSSGGATNPTGGVARTSWKLASPADGTDPTLDFAPEGIVSGSTGCNRFNGTW
                     TEDGDSLKIALGPMTQMACTSDAATQQEKSIIDSLAKVASAEQTATTLVLKDSDGKEL
                     LTYDAVTGDLRGTSWKVNGVNTGNAVETSALTEALTLDFGTEGSVTGFGGCNNFRGSY
                     TLEGGVISFSEFASTKKACEPDVMELEDKYLAALAAASVVERSGNTLNLRDDKGATQV
                     AATLAG"
     gene            complement(12484..13251)
                     /locus_tag="PLZ72_08735"
     CDS             complement(12484..13251)
                     /locus_tag="PLZ72_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HNA36420.1"
                     /translation="MIFGTRRRRRRRELYDAGFTDDWRQLCRDRLDWWDVLAPDERER
                     VELVALGLIARADWEPANGFEVTEDMQVLIAAQAALLTANLPYEDPYRDVHAVIVHPT
                     TAIMHGEHSQVDGIYSDDPTPVLGEAMMHGPLLVAWDEVQDDVHHAGDGRNVVFHEFA
                     HTLDMLDGVTDGTPPMDADLARRWVPVCTAVYEAVVEGRAGEVLDDYAGVNPAEFFAV
                     VTEAFFDDPLGLRDEHPDLYDCFSDFYGQDPAGWFEA"
     gene            complement(13214..14500)
                     /locus_tag="PLZ72_08740"
     CDS             complement(13214..14500)
                     /locus_tag="PLZ72_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNA36421.1"
                     /translation="MGTSAVIIAVTIGLLVGAASAAVVVHLRWARRSAEEGAALRERV
                     VAAEAAVRTDDQLLEAYRSVAGSTLQQQSERLLELAGARYQTLEATALGHWQTQGAKV
                     VQQLEHQAAQLRELEAQRRSESAVLTNAVQELARSSDQVRTEARQLTGALRDNSVRGT
                     WGEVQLRRVLQQAGMERHVDFLEQSGVTRGGADRAGRPDVVVHLPDGRCVVIDAKAPL
                     DRYLDAANCDDDQSQLPLLAAHAKAVGAHAAALSKRDYDSLVPGALDLVVMFLPGDAY
                     LAAASNAQPSLVEDAWARGVVLASPSTLLGFLRGVALGWRERQVAEQAEEIARTGREL
                     HERLSVFAGHLDKVGGSLSRAVDSFNGAVGSLEARVLPQARRFEDLGAGSGRDLGTRS
                     TVDAGPRRLNVVPSITGDSTADSSDDLRNPTPSTAS"
BASE COUNT         2159 a         5184 c         5162 g         2000 t
ORIGIN      
        1 cggagatcgc cggagcgaag cactccagac cgtgcgcgtc ggcggtggat cgcgccgagc
       61 gcgacagggt gacggccgtt gcgagctcac cgtcctgcag cgccagcaac gccagcaacg
      121 ccaggccgac cgcccggaca tacggcgtct ctccgactgc gtcgatcgct tcggagagcc
      181 gctcccgggc gaggtcgggc cgcccgagca tggcgtaccc cgatccctgg gttccggtgg
      241 ccagcgccca cagccggttc acccccgggc cgccggcctg ccgcacgacc tcggcgtcgg
      301 agaccaccct gtcgacgccg cggcttccca tcagcatccg caccgccgcc agcgcgacct
      361 ccaccgtggg ggtgccgtcg gacaggggcc cgtggtcggc gaagcgggcg gccgcccgac
      421 aggaacgctc ggcgccgtcg cgatcgccag ccgcgacggc gcaccaggcc gcggcgatcg
      481 ccgcttcggg gatctcgtcg accgcgccgg ttccgaggag atccaaccac tccccgacct
      541 ggccgagccg gccgtcgagc caacggtcga gcacggagcc gaggatcagc cccgcggcac
      601 gttcacggtc acaggcccgc accgcgtgtc gcacggcgga gtcgacgtca ccggatcggg
      661 cccgcatccc actggcccgg cgctcgagct ccagcgctcg gtccgggcca tcgcgacgca
      721 gccgctggag caggtggtcg cggaacaggt ggtggtagcg gaaccgctgg ccgtcgtggt
      781 cgagcgggat caggaacagg ttcccgtggc cctcgatcga accgagcatg cgagacgagt
      841 cggatcgttc gagcagctcg tccagctcgt cggcggccat gcggtcgagc acggccgagc
      901 cctccatgaa ggagaccatc tcgggctcgg tgccgtcgag gacctcctcg atcaggtact
      961 cggccaccag ccggtcggca ccggagaacg acctcgggtt tcggccctgg tgcaacgcca
     1021 gcgcggccag gtggagccca ccgggccagc cctcgacccg cgtgacgagg tccgccaact
     1081 cctcgtcgtc aaggctcacg ccggcccggc cgaacagctc ggcggcctcg atcagcccca
     1141 tggccaggtc cgatgcctcc acccggtgca cccgaccgtc catccggtgc cgaccgagcg
     1201 cgatcgggat ccggcgtccg accaggacga cggtcgaccc ctccggcacc gacgacagca
     1261 gcatctgcag accggcctcg gcctcggagg acgtgaggtg gtggacgtcg tcgagcacga
     1321 ccacgcacgg cgaccggtcc gcgagcacgc cggccagcgc ggggaagatg tcacccaggg
     1381 ccgaccgacc cgcccccacg atccgggcgc cgtcggccgg ctcgagcggc gcgagccggt
     1441 ccaaggcgag ggcgaggtgg cgccccaggt gcacggcgtc atcgtcggct tcggacagct
     1501 gcacccaggc gtacggccgg tcgtccgcct cggcgcacag caccagggcg gtcgacttgc
     1561 cgaaccccgg cggggccacc acctcgagga tcggagcctc gggttcgagc accgacttga
     1621 ccatccggtc gcggtcgatg agggaccccc tgcgaggggg gacgacgaac gaggtggccg
     1681 ggatgtcgcg tacgcccgcc gacgcggcca gcgacgctgc gggaactcgg ccttcctcgg
     1741 tcattccgga tgacaacgta ctccgcctcg tcgcaccccc gtcctgtaga tactccccga
     1801 tggccctcga ggttcccggc gcaatccgac cgtttccgtg accgtacgcg ccacctgcgg
     1861 cgcgaacggt cacgggaatc gccggcgggt ccgatcagca ggctccccac cgaaggcacc
     1921 gggcgcgagg ctggggacat gcagcgatca tcggacccgg gcccagccga caccgtcacc
     1981 ttcccgggat gcccgacgcc ggtcgcacct ctcggggtgg gaacctgggc gtggggcgac
     2041 tcgcgcacct ggggcatggg ctcctacgac acggcgctgg acgagaccac gatcggcgac
     2101 gcgtggcgga gctcgctcgg cgccggggtc gggctcttcg acaccgccga ggtgtacggc
     2161 tccggggaga gcgagcgcat cctcggccgc ctgctgtcag ccgacccgtc ggggcgggac
     2221 cggatggtca tcgccaccaa gttctggccg gcgccgtggc gttggaacgt ggtcccggcg
     2281 ctgcgccagg cgctcgaggc ctcgctcgga cggctgcaga tcccgaccgt cgacctgtac
     2341 cagatccacg gcccgatcag cctgcggtcc gcctcggcga tggccgaggc gctggcgacc
     2401 gtcgtcgacg ccggactggt ccgcgccgtc ggcgtgtcga actactcggt ccgtgagatg
     2461 acccggatcc actccgagct gcggcgccga ggggtgcccc tggcgtcgaa ccagatcgag
     2521 ctgtcgctgc tgcgcacccg gccgctgagc tcggggctca tcgacgcctg ccgagacctc
     2581 ggcgtggtgc cgatcgccta ctcgccgatc ggccagggcc gactgaccgg caagtactcg
     2641 gcgtcgaacc cgccgccggg ccgacgcaac ttctcggcgc atccgatgga ggcggtggac
     2701 cgggtggtcg ccgtcgcccg tgagatcggt gacgcgcacg acggccggac ccccgcccag
     2761 gtcgcgttgc gctggatcat cgacaagggc gcggttccca ttcccggcgc caagaacgag
     2821 cgccaagccg tcgacaacgc cggcgccctg ggctggtcgc tcgacgcggc cgaggtcgcg
     2881 gcgctcgacc gggtcgctct ggccggcacc gactcgatcg ccaaccgcgt ctggcagcac
     2941 ggataggcgc ccgagcgcga tgaccgggtt cgcaccatga ccgggttcgc agcatgaccg
     3001 aggtgatcga ggcggtgacc gtcgtcgatg ggacgggagc gccggcgttc atcggcgacg
     3061 tggcgatcga cgaggagcgc atcgtcgagg tcgtcccccg ggagggcgcc ggatcgggtc
     3121 tggtgctcgc tcccggcttc atcgactccc acacccacga cgacctggcc gtgctcgtgc
     3181 acccgcagat ggagttcaag gtgctcggcg gggtgaccac atgcgtggtc ggcaactgcg
     3241 ggatgggcgc cgctccgttc ccggcggccg ccgcgatggg tgcggtgctg cacccgggcc
     3301 acgacctcga cccgtgggac ggctaccgcg ggtacctgcg ccgggtggac gacaccgcgc
     3361 cctcggtcaa cgttgccgcg ctgatcgggc acggcaccgt ccgcagttcg gtgatgggcc
     3421 tcgacgacgg tccgcccgac gacgaccaga tcgccgcgat gagccgtctg atcgacgagg
     3481 gcatggctgc cgggtgcgtc ggcatgtcca cggggctgac ctacgaaccg ggcatctggg
     3541 cccgcaccga ggagctgacc gctctcgcgg ccgtcgtcgc cgcggccggc ggcatctaca
     3601 ccagccacat ccgcgacgaa gggtcgcacc tgatcgaggc ggtcgacgag gcgatcggga
     3661 tcggccggga cgccgggctc ccggtggtcg tctcgcacct gaaggccatg ggagagccca
     3721 actggggcac ggtcgccgct gcgctcgagc ggatcgacga cgccggtccc gccgtcgcgg
     3781 cggaccagta cccgtacacc gcgagctcga ccatgctcgc cgcggtcgcg gcgtccgggc
     3841 ggatcggaac gctggtcacc cccgacgccg tcgtgatcgc atcgacgacc gaccacccgg
     3901 agtggcacgg gcggtcgttg gccgagctgg ccgaccgcct cggggtcccc ggcgaccggg
     3961 tggcgacggc ggtcctcgac gcggagccgg ttgcgaccgt gatcgtgcac tcgatgtcgg
     4021 aggacgacgt gcggaccgtg ctcggacatc ccgggatcat ggtcggcagc gacggcatcc
     4081 ccagcctgga gggccacccg cacccccggc tgtacggcac gttcgcccgg gtgctcggcc
     4141 gctacgtgag agacctgggg gtcctcgggt tggaggaggc ggtgcaccgg atgacgggtc
     4201 gacccgccga ggtgttcggg ttggtcgacc gcggggtcat ccgccccggc gccgtcgccg
     4261 acctggtgct gttcgacccg gcgaccgtcg tcgaccgggg caccttcgac gatccccggc
     4321 agtcgcccga cgggatcgtc ggggtctggg tcaacggcgc ccgggtggtc accgacggcg
     4381 tgcacctggg cacccgctcg ggccgggcgc tgcgtcgacg gcgcgagcgc gtctcacgca
     4441 cgggttcggc gtcggcgtga gcgaggagcg ctcccatcgt cagggacacc acgacgctca
     4501 ccaggaggaa caccgccgcc gctgacgtga tcgtgatgct gcccatggtc cgacctcctc
     4561 gtccgatgtc gacccggcgg cgggagccgg gcgggtcctg acggggatca tcctcatcgg
     4621 cgggaggtcg tggcgtaatg gggacgactc cccgccgacc cgttctgtag tgcgagatcc
     4681 aggtgttcgc ctggatctcg cactacagaa cggggttttg gggcttgggg gcgcggggcc
     4741 gtcaggggcg ggcgcgggtg cggcgcagca gcgggtcgcc cgcagccagg acgcggtccg
     4801 cggcccgcat ggtccagccg ccggcgcccg gagtggagcc gtcgaccagg ttgcccatga
     4861 cctgcagggt gatctcggcg acggcctggg tgccgaccgc cacccgcagt ccgttgcgca
     4921 ggatccaggg gtggccgatc aggaagctga accgtcgacc ggtgcgcagg aacgcgtcga
     4981 agcgctcccc cacctcggcg tcgtagcgca ccggcgcggt cacggggtcg gccaggaaca
     5041 ggtccgcggc gagggtgccg gactcgaggg cgtagtcgat cccctcgccg ttcatcgggt
     5101 tgatcaaccc ggccgcgtcg ccgatcgcga cccagccggg accggaccgt cgacgggcgc
     5161 tcatcggcag acgccacgcc cggggacgtt ccaggtcggg gccgagctcc cagtcacggc
     5221 cgaccagggc ccggtagctg tcctgcaacg cgttcaggtt gagcttcttg aagcccttca
     5281 tggtcgacag ggccccgacg ccgatgttca ccgtcccgtc accggccggg aagatccatc
     5341 cgtagcccgg caccggcgtc cccgacccgt cgcggagggt caggcacgcc tcgaggtagc
     5401 gctcggcgtg acggggcgac tcggcgtagg tccggatcgc caggccgaac gtctcgtcgg
     5461 gcacccgctc ggcgccgagc agtcgggccg ccgcgcccgg cgcacccgcg gcgaggacga
     5521 ccagcggagc ctcccaccga cgggtccgac cgtcggcgtc gaccgaggtc accccggtcg
     5581 cccggttggg tgccgcaccg acaccctcgt cgacgacggg gaccgcgtcg tggctccaca
     5641 gcaactcggc tcccgcggcc gcggcccgct ccatcagccg ttcgtccagt cgccggcggg
     5701 gccacaccgc gccgtgggcg gggaacggcg cgatccccgg ccagtccatc tggcggacgg
     5761 tcgcgttggc ggtcatccgc aggccgtcga tgcggtgggc gtcgtcgaga gcgacgtcga
     5821 gcgcgtcgag cgcccggacc gcccgcgggg taaggccgtc gccgcagatc ttgtcccggc
     5881 ccggtcgccc cttctcgaac acggtgaccc gggcgcctcc cgccgcggcc cgcatggcga
     5941 cggccgcgcc ggcgggaccc ccgccgatca ccagcacgtc ccgggactcc gccgtcgagc
     6001 tcaccccgac agtcaaggcg acccggcgcc gcggagcgaa gtccgggccg ccggtcccgt
     6061 catgccggcg cgcccttgga acgagcctcg gggatgagca tcgccgcggc cacgaagaag
     6121 cacacgcctc ccgccacggt cccggcgttg tcgagccact cgttgaccag ctcaccggag
     6181 ccgagggtgt acgccccgac agccgagacc ccgaagaaga ccgagccgag gaggttcagc
     6241 acgacgatcc accacgacac gtcccggagc cggagccgtc cggcggagtg gcagacctcg
     6301 gcccacgcca ggtagctggc gacgaggaag cagatcgagc cgatggtgtc gggacgccac
     6361 accagccggt tggcctgggc cgtgtcccac ccgtcgtaca tggccaggaa cgtcatgacg
     6421 ttgaagtaca cggtgccgac cagctgcacc ccggtcgacc accagtcgat cgagtgcggc
     6481 cgccaggaga tcagccggaa cggaccgcgg tggcgacgct gcgggtcgtc gacgaccgca
     6541 tcgggagcgt tggaggcctc gacgaagcag aggtacgagg cagaggtgaa gaagatcgac
     6601 ccgacgaaga acgtgagccc gacccaccgg gtgcccaccc acgactcgta cgccggcagc
     6661 ggcccgacgg tgaagcacgt cgagccgatg cagaacagca ccgcgatcca ccacgtccgc
     6721 cgcggaacgc cgggacggtc gagacccttg cggtgatccc gcgagcgcca cacccggatg
     6781 gtcccgtcgg ccaggcggta gcggatccgg gtgacgaacg gaccgaaccc gcgggtcgac
     6841 tcgatggtcc agcccgacgg ggtcgggacc ggcacccgtc gccggtcatc catcgcggtg
     6901 ctcccacatg accggacagt ctgccgatcg gcgggggtgc tgggcgtcac ccgatcgggg
     6961 cggtcggacg gggcgatcac gcggggtggt cgcgactgcg cgacgctcgg ggcctcacga
     7021 cctcgacggt tcccgtactg tcgcggcgtg ggcctctacg acgatcgggt gctgccccgg
     7081 ctggtcgacc tgacctgcgg gtcgaagggc atggagcgct accgcgccaa ggccatcgac
     7141 gggctggccg gcgaggtggt cgagatcggc ttcggcaccg gcctcaacgt gccgctctac
     7201 cccgacgcgg tcacgaccgt ctgggcggtc gagccgtcgg agcgttcacg tgagctggcg
     7261 tcggaccggg tgacggcacg aggcgttcgg gtcgagcacg tcggactcga cggccagcga
     7321 ctgccgctcg acgacgccag ctgcgacgcc gggttgtgca ccttcacgct gtgcacggtc
     7381 cccgatccga tgcgggcgct ggcggagctc cgtcgggtga tccgccccgg tgggcagctg
     7441 cacgtgctcg agcacggcgc cgccccggat cccggtatcg cccgttggca ggagcggatc
     7501 gagccgtggc agaagcgcct ggccggaggc tgccacctga cccgcgacac gaccgagatg
     7561 ctgcgccgcg ccggcttcgt cgaactgcgc ctgaccaccc ggttcgtgaa gggccccaaa
     7621 ccctggagct acttcacctg gggacgggcc accaaccccc gctcccccga cgttctgtag
     7681 tgcgagatcc aggcgaacgc ctggatctcg cactacagaa cgagcacgtg ggggatgccg
     7741 aggcgggcgt cagagccagt cgcggcggcg gaacatgagg tagagcgtga ccgacgtgac
     7801 cacgatcaac gcgaccgcca gccacgcggc ccacgtcgtg cccagtcccg cgaagcggac
     7861 gttcatgccg tagaagccgg tgacgagcgt cggcaccgcg atgatcgccg cccagctcgt
     7921 caccttcttc atcacctggt tctggcggta gtcccgcacg ctcaggttgg tctcgacgat
     7981 cgtcgccacc aggtcccgca acgagtcggt cgactcggtg atgcgcagga cgtggtcgta
     8041 gaggtcctgg tagtagggct gcagggccgg gccgaccacg tcgtgctcgc ggcgcatgag
     8101 ggagccgacc gcctcgcgca gcgggaacac cagccggtgg aagcgcacca nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcacatgtcc acccagaccg tggtgtcgtc
     8281 cacctcgagg aagtcggaga cctgctcgac cgggaagtcc ttctcgacga gcttgccgtc
     8341 gcggtaggcg cgggtctgca cggagtcgct caccgctcca ttcaagccgt cgccgggagc
     8401 tgcgccgcgc aaggtcggtc gaccggcaca cctcgatggt tcaggtgggg cgctcgcgcg
     8461 ccgatgaagg accagcacca gcagagcccg tggctcggtc ggatcggagg cagccggtcc
     8521 gacccacagc cacgacccac cgcggacggg acaccgatgc agcagaacga ccacggctcc
     8581 gacgaccaga tccccgacgt caggacgccc accagccccg aggccctggg tctgagccgg
     8641 tccctgatgg aggacctggt gatccgccgc gccgtcaacg acggccggtc gccgatgacc
     8701 gctctggccg acaagctgca cgtctcgatc accgtggtgg agtccctcct caactcgctg
     8761 cgcgaccgga agctcatcga gtacgacggc atggaaggcc gcgcctacgt ggtgcgcgcc
     8821 accgaggccg gccagcagtt cgcgacccag cgatcccggg agagcatcta ctgcggcccg
     8881 atgcccgttc cgctcgatct ctacacagcg gtcgtgcgcc accagcgtcc gcagatcgaa
     8941 ctggaccacg accgcttgca gcgctcgttc tcggacctgg tcctgtcgga cgggctgctc
     9001 gaccagctcg ggcccgcgat ccacggcgac ggggcgatgt tcctctacgg cccgcccggc
     9061 accggcaaat cctcggtcgc cgagcggatc atcagggcct acgaggacgt ggtgctggtc
     9121 ccgcactgcg tcgaggtcga cggccagatc gtctcggtgt tcgacccgac gctgcacgac
     9181 gagatcgacg agcagccccc cgggatggac cgacgatggg tggcatgcca ccgacccgcg
     9241 gtcatcaccg gcggcgagct ccacgccgga atgctcgacc tccagttgga gcgggacagc
     9301 ggcgtgtacc tggcgccgct gcagatgaag gccaacaacg ggatcctggt gatcgacgac
     9361 ttcggtcggc aggcgatgac gcccgacgcc ctgctcaacc gctggatcgt cccactggac
     9421 cgcagcgtcg actacctgac cctgctcgga cgcaagttcg aggtcccctt cgagctgaag
     9481 gtcgtgctgt cgaccaacct cgacccggcc aagttgggcg acgaggcgtt cttccgccgt
     9541 atccacaaca agatctacat cggggcctgc accgacgagc agttcgactg gatcctgtcg
     9601 cgggtgaccc gggcgacagg cctggagctc caggcgcccg cagcggtgcg tctccgccag
     9661 gccgcccgct cccagggcga cggtgagctg cgggcctacc tacccggcgt ggtgtgcaag
     9721 ctggccaacg ccatctgccg ctacgagaac caggcgcggg tcctgacccc cgagctggtc
     9781 gatcgcgtgc tggcgctgta cttcaccgag gacctcggcg tggagcgcac cggaccggca
     9841 ccgcatcaca ccgtcgacgt gccgcccttc gcccccgacc cggagctcga gtccgagatg
     9901 gcccggctgg caggtcctgg cccggagcag gacagcgatg cgccgctcgg agcggcaccc
     9961 gaggcgttcg atgcacctgt cgatgccgac ggagccgacg cgccggttcc tcccccgtat
    10021 ccggggatgt tcaccgggtc gctggacccg gccgcggcga ccatcgccga ggggttgttg
    10081 gccgacggcc tccaaccgcg ctgaacctgg ccggccgagg agcgttccga cggagcgcgg
    10141 atgcgggatc agatagggac cgactccaac aatcgggtga caggccggcc gacgagcgcc
    10201 ggcgagctgg tcgggtcggc ccacaccacc tcgcggagga agtcccggaa ctccgcagcc
    10261 cgctcagccc cgtcgaagtc caactcgacg acgatgcggg tcgggtcgtc gagcggatgg
    10321 gcgatccggt gcgacctcac ccccgcgtgg cgtcgggcgt cggcgaaccg ttcgaacgcg
    10381 gccgtccagg tcgcgaggtc ggtgatctcg tgttcgatga gcaacgtgac catggttcga
    10441 cggtacggag gacggggtgc tgcgaccatc ccaggtagct gggatcgccg aaggaccccc
    10501 gggccgggct cgacgccgcc gatctagcgt gcggccgtgt cgatcgaagg tctgtgtgcg
    10561 acggtccgct ccgagcgcga cctgcggatc gcactgctgg acgagctgcg acgctcggtg
    10621 cccttcgacg cgtacgcgtg ggtgctcacc gatccgcgca ccgaggtcgg ggcatcgccg
    10681 gtcgccgacg tgccatgtct gtcggagctc ccccgcctga tccgactcag gtacgcgacg
    10741 gagcacgccc gatggaccgg cgccggtgac ccggtggtga cgctggtcga ctcgacagcg
    10801 ggcagaccgg agcagagccg gctgtaccgg gagctcctcg ccgacctcgg cgtcagcgac
    10861 gttgcatcgg tggtgttccg cgatgtcaac gggtgctggg cgttcctgga cctgtggcgc
    10921 atggacggga ccttcgaccc ggccgagacc cgactgctgc gatccggcgt cgagcccatc
    10981 accgccgccc tgcgacggtg cgtcctccac acgttcgccg acaccgatcc ccacgtcggc
    11041 aggtcgctcc cgacgccggt ggtgatcctc ctcgacgacg acctgcgggt ccgggagcag
    11101 accccgaccg cagaggggta cctggcgacg ctcctcccgc cggaagcgga ccaacgagcg
    11161 atacctgcgg gtgcctacaa cgtcgcggcc cagctcctcg ccgtcgagga cggcgtcgac
    11221 gaccacgagc cggccgcccg ggtgcacgtc gagccgctcg actggctcgt gttccgggcc
    11281 gcccgactcg gcgacgacat cgcggtgacg atcgagcggg catcgccctt cgaacgcctg
    11341 gagttgttcg cacgtgcggc cgggctgagc ccacgggagt ccgagctggt cgagcacctc
    11401 acgaccggag ccgacacccg gcaggtcgcg gcgctcatgt tcgtgtcgga gaacacggtc
    11461 caggaccacc tcaaggcggt gttcgccaag accggcgtcc ggagtcgccg cgagctgctg
    11521 gcccgagtcg cgggacggtg agatcagccg gcgagggtgg ccgccacctg ggtggcgccc
    11581 ttgtcgtcgc gcaggttcag ggtgttgccg gagcgctcga cgacgctcgc cgcggccaga
    11641 gcggccaggt acttgtcctc gagctccatc acgtcgggct cgcaggcctt cttggtggag
    11701 gcgaactcgc tgaacgagat gacgccaccc tcgagcgtgt agctgccccg gaagttgttg
    11761 cagccgccga agccggtgac cgagccctcg gtgccgaagt cgagcgtgag cgcctcggtc
    11821 aacgcggacg tctcgaccgc gttgccggtg ttgacgccgt tcaccttcca cgaggttccg
    11881 cggaggtcgc cggtcaccgc gtcataggtg agcagctcct tgccgtcgga gtccttcagg
    11941 acgagggtgg tcgccgtctg ctccgccgag gccaccttgg ccagcgagtc gatgatcgac
    12001 ttctcctgtt gcgtggccgc gtcggaggtg caggccatct gggtcatcgg accgagcgcg
    12061 atcttcagcg agtcgccgtc ctcggtccag gtcccgttga accggttgca gccggtcgag
    12121 cccgacacga tgccctccgg cgcgaagtcg agggtcgggt cggtcccatc tgccggggag
    12181 gcgagcttcc acgacgtccg cgccacaccg ccggtcgggt tcgtcgcccc gccgctgctg
    12241 ggcttggcgg tggtcgtcgt ggtcgaccgg tccttgtccg ccgtcacgtc gtcacctccg
    12301 caggccgtga ggaccccggc gagtgccagc agggcgaacc ctgcggcgat tccacggcgg
    12361 agggttcggt gggagctcgt cggggtgtac atcccgacac gatggcacac cgaccgtcgg
    12421 ctcggggcgc ggtgagggtc ggtcgaggtc aggggttcga gacagcggga cagcggtcgc
    12481 cgatcacgct tcgaaccacc cggccggatc ctgcccgtag aagtccgaga agcagtcgta
    12541 gaggtccggg tgctcgtcgc ggagtccgag cgggtcgtcg aagaacgcct cggtgaccac
    12601 cgcgaagaac tccgcggggt tgaccccggc gtagtcgtcg agcacctcac cggcgcgacc
    12661 ctcgaccacg gcctcgtaca ccgccgtgca gaccggcacc caacgccgag ccagatccgc
    12721 gtccatcggc ggggtgccgt cggtgacgcc gtcgagcatg tcgagcgtgt gggcgaactc
    12781 gtggaacacg acgttgcgcc cgtcaccggc gtggtggacg tcgtcctgca cctcgtccca
    12841 cgccaccaac aacggtccgt gcatcattgc ctcgcccagc accggggtgg ggtcatcgga
    12901 gtagatgccg tcgacttggg agtgttcgcc gtgcatgatc gccgtggtcg ggtggacgat
    12961 cacggcgtgg acgtcccggt acgggtcctc gtaggggagg ttggcggtca gcaacgccgc
    13021 ctgcgccgcg atcaggacct gcatgtcctc ggtgacctcg aagccgttgg ccggctccca
    13081 gtcggcccgg gcgatcagcc cgagcgcgac cagctcgacc cgctctcgct cgtcgggggc
    13141 gaggacgtcc caccagtcga gacgatcccg gcacagctgt cgccagtcgt cggtgaaccc
    13201 ggcgtcgtac agctcacgac gccgtcgacg gcgtcgggtt ccgaagatca tcggaggagt
    13261 ctgccgtgga gtcgcccgtg atcgacggca ccacgttgag ccgccgcggt ccggcgtcga
    13321 ccgtgcttcg ggtgcccagg tcacgaccgg agcccgctcc gaggtcttcg aagcgccggg
    13381 cctgaggcag gacgcgggcc tcgagcgagc cgaccgcgcc gttgaacgag tcgacagcgc
    13441 ggctgagcga gccgccgacc ttgtcgaggt ggccggcgaa caccgacagg cgttcgtgca
    13501 gctcacgacc ggtgcgggcg atctcctcgg cctgctccgc cacctgccgc tcgcgccagc
    13561 cgagggcgac cccacgcagg aagccgagca acgtggacgg ggaggccagc accacacccc
    13621 tggcccacgc gtcctcgacc agcgatggct gggcgttgct cgccgcggcc aggtaggcgt
    13681 cgcccgggag gaacatgacc accaggtcga gcgcgccggg gaccagggag tcgtagtcac
    13741 gcttggacag cgcggcggcg tgcgcgccga cggccttggc gtgcgcggcc agcaacggaa
    13801 gttgtgactg gtcgtcgtcg cagttggccg cgtcgaggta gcggtcgagc ggcgccttgg
    13861 cgtcgatgac gacgcaccga ccgtcgggga ggtggaccac gacatcggga cggccggcac
    13921 gatcggcccc accccgggtg acccccgact gctcgaggaa gtcgacgtgg cgttccatgc
    13981 cggcctgctg caggacccgc cgcagctgga cctctcccca tgtaccgcgc accgagttgt
    14041 cgcgcagggc ccccgtgagc tgccgggcct cggtccggac ctggtcgctg ctgcgggcca
    14101 gctcctgcac ggcgttggtg agcaccgcgc tctcggagcg gcgctgcgcc tcgagctcgc
    14161 ggagttgggc cgcctggtgc tcgagctgct ggacgacctt ggcgccctgg gtctgccagt
    14221 gaccgagcgc cgtggcctcc agcgtctggt agcgggcacc ggccaattcg aggagccgtt
    14281 cggactgctg ctgaagggtg gagcccgcca cggagcggta ggcctcgagg agctggtcat
    14341 cggtgcggac cgccgcttcg gccgccacga cccgctcccg cagcgccgcg ccctcttcgg
    14401 ctgagcgacg ggcccagcgc aggtggacga cgacggcggc cgacgccgca ccgacgagca
    14461 gaccgatggt gacggcgatg atcacggcac tggttcccat ggccacgcat ggtgcacgcg
    14521 gggtgtgaca ccgcaactga gcgaccgttc tcatgacagt tgagtaccgc cagtgtcgtg
    14581 acttgtcgtc agaaactcgg ggttg
//
LOCUS       DAOQOD010000074        14249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1514_length_14249_cov_11.458926, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000074 DAOQOD010000000
VERSION     DAOQOD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_14249_cov_11.458926"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PLZ72_08745"
     CDS             complement(<1..1545)
                     /locus_tag="PLZ72_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNA36422.1"
                     /translation="MTAEPRPGGPDPVVHLPQRDRPWLMRTYSGHSTARASNELYRTN
                     LAKGQTGLSIAFDLPTQTGYDPDHVLARGEVGKVGVPVAHLGHMRTLLDGIPPGEMNT
                     SMTINATAAWLLGLYTANATEQGVATTALRGTTQNDIVKEYLSRGTYVFPPGPSRRLI
                     VDMFAFCNEHIPKWNPMNVCSYHLQEAGATPVQEIAYSLACALGVLDAVKESGQVAPE
                     RMPEVVASVSFFVNAGIRFVEEIAKMRTFTRMWDRLTAERYGITDPKARRFRYGVQVN
                     SLGLTEAQPENNVQRIVLEALGVTMSRDARARSLQLPAWNEALGLPRPWDQQWSLRIQ
                     QVLALETDLLDYPDLFEGSVVMEGLTSELEDAAQAELDEVLELGGAFEAIDELKGRLV
                     RSHTERMRRIESGELTVLGVNAFTETAPSPLAGDDSILKVDPAVQDEMIEDVRRWRTE
                     RDKAAVDKAMRELERVARSDENIMGPTIELARVGGTTGEWADCLREVFGEYRAPTGVA
                     AAAGVAG"
     gene            1978..2208
                     /locus_tag="PLZ72_08750"
     CDS             1978..2208
                     /locus_tag="PLZ72_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36423.1"
                     /translation="MIALLLAADDGSGALFLGEDFFPWMILAFGAAMVVGNILALVRP
                     AADGQRAPAGRAAAMIVVGAIAAVWGAVSLLS"
     gene            complement(2263..2886)
                     /locus_tag="PLZ72_08755"
     CDS             complement(2263..2886)
                     /locus_tag="PLZ72_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA36424.1"
                     /translation="MTIELVRTDGVFALDGGEWEVTNNIWLVGDDRSVLIVDAAHDAD
                     VIAEAVNGRRVTAIALTHGHNDHINAAVALRELVDAPVLLHPADRMLWDAVHPGADPD
                     GDLAAGDVLRAGPAELAVLSTPGHSPGCCSFHDVSGGHVFSGDTLFCGGPGATGRSFS
                     DFPTIITSIREQLLTLPPETVVHTGHGESTTIGAEAPNLEEWVRRGH"
     gene            complement(2891..4663)
                     /locus_tag="PLZ72_08760"
     CDS             complement(2891..4663)
                     /locus_tag="PLZ72_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36425.1"
                     /translation="MTRTLPAPPATRMVAACLLAAVALITSGCTQRSDDARMESDPRP
                     ATSGGSAAPATSLDWAPCRGKLTSQAGLQCATLAVPIDPSTPDGPTLDLALARKKSTG
                     SADERIGSLVMNPGGPGGSGLEFLANASMAFPSSLTDRFDLVSFDPRGVGESDPVRCL
                     DDDQKDEQLEGDLSPDDDAERAHSEAQQAELRKGCETRNPDLVTHMSTADVAADMDRI
                     RAAVGDEKLNYLGYSYGTAIGATYAAKFPANIRAMVLDGSVSPSASVADQAMVQATGF
                     ERTLGNFVTTCDADKSCALAPDTAAAIESTRRSLEEKPVEVKTGAGTRTLTSDLFDYG
                     LATALYDTSTWGPTAKAIKNIRSGGAKTILALVDRQTGRQADGSYDNSSDAQTMVNCA
                     DQNDHLTEEQARVEEEKIRAAAPTFGGLLGAGLTGCNDWPAPAEPTPAPSAVGAPPIL
                     VIGTVGDPATPYEWAQEMSAALTGSVLLTYEGDGHTAFLRGGPCIEDAVVDYLVDLTL
                     PAVGTRCPAQSTGGGFGGTRDMLVEQLTGSGIPKEMATCMVDGMIEKVGEAEFDDMVL
                     NNDVDGITKLAQAAALGCATSGGG"
     gene            complement(4790..5470)
                     /locus_tag="PLZ72_08765"
     CDS             complement(4790..5470)
                     /locus_tag="PLZ72_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNA36426.1"
                     /translation="MPVLDLADRHARHRQNVLLVAVGAYIGVSLMANVMSVRIVLLGP
                     WAIDAGTFTYPLTFTLRDVVHKAGGRTAARVTIVTGAALNLVMVAAFWVTANLPADQT
                     VGPQTEFGNLLIPVARIVVASIVAQVVAELIDTEVYHRVMERLGPRHQWARVVASNAV
                     SIPTDSVLFAIIAFAGDVPTAALVQIMATNVGLKAATTALTWPLIYTVREPGDPEDPA
                     ANAPVPVP"
     gene            5534..6505
                     /locus_tag="PLZ72_08770"
     CDS             5534..6505
                     /locus_tag="PLZ72_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNA36427.1"
                     /translation="MPDVDDAPPDRGTTRDRPAPSPPSVYPPDDHVLRDLPFEMEETG
                     PRSAVSHLRLTPNHCDHGRVDPATLLVLTDVLGGFLVGRVIAPDWMATAQLALHMFEH
                     PGVGELRIGATVVRAGRSTVVVSADIAVGPARPAGHGVSGAGSDGPRRVGAAELTFVR
                     LPRRDTTLDLPTDGPSAGRRLSLAVPGSGLTAPLRRSIGVDEPGGSVGLEVTPYVRNS
                     FGAVNGGVVAALAAAAAEGAAAERTGVPVRVADVAVTYLAQARVGPVEASASVLRVDG
                     PVALVRVEVRDPGLPDDDGRPRVAVTAHVHCVTDADAGSGRGSLRDS"
     gene            complement(6492..8354)
                     /locus_tag="PLZ72_08775"
     CDS             complement(6492..8354)
                     /locus_tag="PLZ72_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNA36428.1"
                     /translation="MEIARHGMMSPMRHHVRVVAALLVTATLAVGLAACTRDPDVTGL
                     PSELPPADGATTTVPLPVAAPPCVQTPNGVGQDPPPGSRPGDLIASYELDGQYKSSVG
                     FPTDAHAWRISYVSTGLDEHDLQIVCGMAAAPAKGPSTFTVDSGPVGRMLAWAHGTSG
                     LQQACLPSLAPDTSLWGKMSDGINAISWGSGFGARKGDPANGALQTALDRGWVVSAAD
                     YQPNDTYVVGRIAAANVIDAARATSQLMDQQHPGEAPDAYDTITWGHSQGGHAAVWAG
                     QMMDSYQEAAPNHDAATLRLAGVAALAPAANFIVQPDRQGIEAGNGLADWEMHHTIEM
                     LGLPLPALELQIAPPLFSYIFGSWAVLSGRGTPADDATTPAFPRDAGVLDLRAVTTAE
                     GAETVAQLAALCTVTDARKVQSIAAPYRDAARHKMLVPELWNLPDAYSPGEFFKGGVD
                     RACATRDPERSDGGGLGDWCRWIRWNLPGPDGVNPFPKVPEQDGAPVPMLIGQGMDDQ
                     VIHCRPADGTPDADVPAAADCMSVALYESLRTADDGYCPATGDRGHLELALYRKDGSA
                     SPAGHLPIPGQISAAGSGKAAADLTFTGSPMERFMTGAFDRTLGTGCTARVMNP"
     gene            complement(8408..9343)
                     /locus_tag="PLZ72_08780"
     CDS             complement(8408..9343)
                     /locus_tag="PLZ72_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNA36429.1"
                     /translation="MTITDGNLQWTEHPDPEPEAGELLVAVRAAGLNAGDLLQLAGHY
                     PAPAGSPADIPGLELAGEVLAVGTGVQRYAVGDRVMAVVGGGAQAERAVVHESCALPV
                     PDTLDWAGAGGFPEAFTTAHDAVFTQAGLAVGETLCVHGAAGGVGVAAVQLGVATGAH
                     VVATVRNPDLRDRVAELGATVVAPERFAAHGPFDVVLELIGGPNLRDDVRALSIGGRI
                     TVIGVGAGPKAEISLLDLMAVRGRIHGSTLRARPLEQKAAAADAVRRHVLPLVTRGRI
                     HVPVAATFPMSAAAEAYERFRSGGKFGKIVLVAGN"
     gene            complement(9441..10109)
                     /locus_tag="PLZ72_08785"
     CDS             complement(9441..10109)
                     /locus_tag="PLZ72_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36430.1"
                     /translation="MAEQLRTSRRIDAMGSAVTGAWHHRAMTVWAVLALGFAAAFVAA
                     MVAAARGRSTKFVGSLAGLIALACGWVWWSDARMEARLSQAASAVAGVQVHVDCQELF
                     FGEWMPTRGAGRVDARDDGSMDDVAHLDRKICTAAASWPEDDTDDAYTAVHVLTHEAG
                     HVRGEANEAATECWAVQNSARVAEALGASRPRSERRAQWYLRAVFPRMSSTYRGDGCT
                     ISGS"
     gene            10173..10556
                     /locus_tag="PLZ72_08790"
     CDS             10173..10556
                     /locus_tag="PLZ72_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HNA36431.1"
                     /translation="MTPQEVVAGFIAAIESRDVDAALALLAEDVSYENMPMDPIVGRE
                     MTGVVLHSYLDLSTEVEWRIVREVLDGDVVINERVDRFHIGDGWLELPVAGFWEVRDG
                     LITLWRDYFDMSAYTSQLDALVGDG"
     gene            10767..11486
                     /locus_tag="PLZ72_08795"
     CDS             10767..11486
                     /locus_tag="PLZ72_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36432.1"
                     /translation="MDPRSQLTARLSTELDDPDGARSVVAAMDDAAVAAAEALADGDI
                     AGSPWLSYRPSTVPVDEIDSLWVFSYGFRFADPAADPVGLGARVPGPDELVPGPTNEA
                     LARAAADVVVDHPVPVVAQWEVARELQKLGVADVISVEPDTAPDGSVVYLSTAGVADK
                     GLRQLTAAGTEPGQAGVLCFRYHAERCVLTARAAGLTADVPEGADLPSKFDPKSGQDW
                     TRSLETWIPVDLAGRTVLKAG"
     gene            complement(11773..12537)
                     /locus_tag="PLZ72_08800"
     CDS             complement(11773..12537)
                     /locus_tag="PLZ72_08800"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA36433.1"
                     /translation="MSADVPTVVLPGPAMVPLVRAAAALEAEDIPPFAVIGGVAVTVR
                     LGRAVRATADLDAVTDQRYTPTALEVLRARGDATYDAAEPHTITIAGAEVQFQDVEPV
                     DDADVSHLDDKDLLYVAAHAHALAAATPVRLLAAETRTEATVPVATTGALLATKLHAY
                     LDRRRVAGRDKRPGDLWDIYNLLLHLGGDAAADLAGANPSLIRVVATTVRDQLVAGAA
                     RARSVLLISNDERYQVITAEEISYAAVAFLDSLGER"
     gene            complement(12527..13573)
                     /locus_tag="PLZ72_08805"
     CDS             complement(12527..13573)
                     /locus_tag="PLZ72_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36434.1"
                     /translation="MGRRSTDVLDRSLREQAMAALVEAVGGVQPASNSTDDLEIELDG
                     HRVTIEVKTMAYATVDRVEALKRKGVPTGDKSILVVADRINQPAQAALKEAGWGYLDL
                     ATGSLFLRAPGIRIDTTVDAVEPGERPKPVGVVGRAGRVVAYELLRRRYDEDPRPVLT
                     TTSFDQFDLARSSTSDARRALIGAGLLTEDGEPVLPELFWALAREWAPTDRRWLASVP
                     DPKDWDRAHDPTEPTWRLGGAQAAIELGAPIVTAADGPIDLYVPGPVLLSIAVRRYGS
                     SDPLAAAASVAVPAVHQVTAPPDPASGERRHRGWLLVHPVAAALDLAAAGDARSLQVL
                     AEWRPDGEAVWHER"
     gene            13814..14044
                     /locus_tag="PLZ72_08810"
     CDS             13814..14044
                     /locus_tag="PLZ72_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36435.1"
                     /translation="MAADSALAVQARLHRDAGLEPPDPIAATRAALADQRGADAALNS
                     PDAPTVVEPEPKPLIAPTAPPPTRSTGPSLGL"
BASE COUNT         1891 a         4993 c         5237 g         2128 t
ORIGIN      
        1 gccggccaca cccgcggcgg cggcgacccc ggtcggggcg cggtactcgc cgaacacctc
       61 gcggaggcag tcggcccact cgccggtggt cccgccgact cgggccagct cgatcgtcgg
      121 gcccatgatg ttctcgtcgg agcgggcgac ccgttccagc tcgcgcatcg ccttgtcgac
      181 cgcggccttg tcgcgctcgg tgcgccaacg gcggacgtcc tcgatcatct cgtcctgcac
      241 cgccgggtcg accttgagga tgctgtcgtc gcctgcgagc ggtgaggggg cggtctcggt
      301 gaaggcgttg acgccgagca cggtcagctc gccgctctcg atccgacgca tgcgctcggt
      361 gtgggagcgc accaggcgac ccttgagctc gtcgatcgcc tcgaaggcgc cgccgagctc
      421 gagcacctcg tcgagctcgg cctgagcggc gtcctccagc tcggaggtga gaccctccat
      481 gaccaccgac ccctcgaaca ggtcggggta gtcgagcagg tcggtctcga gcgccagcac
      541 ctgctggatg cgaagcgacc actgctggtc ccacgggcgg ggcaggccga gcgcctcgtt
      601 ccacgccggc agctgcaggg atcgggcccg ggcgtcgcgg gacatggtca cgccgagcgc
      661 ctcgagcacg atgcgctgca cgttgttctc cggctgcgcc tcggtcagac ccagggagtt
      721 gacctgcacg ccgtagcgga agcggcgggc cttggggtcg gtgatgccgt agcgctcggc
      781 ggtgaggcgg tcccacatgc gggtgaaggt ccgcatcttg gcgatctcct ccacgaagcg
      841 gatgccggcg ttgacgaaga agctcaccga cgccacgacc tcgggcatcc gctcgggcgc
      901 gacctggccg gactccttca ccgcgtcgag gacacccagc gcgcacgcga gcgagtaggc
      961 gatctcctgc accggcgtcg ccccggcctc ctgcaggtgg taggagcaca cgttcatcgg
     1021 gttccacttg gggatgtgct cgttgcagaa cgcgaacatg tcgacgatca gtcggcggct
     1081 cggccccggc gggaagacgt aggtccccct cgagaggtac tccttgacga tgtcgttctg
     1141 ggtcgttccg cgcagagcgg tggtcgccac gccctgctcg gtggcgttgg ccgtgtagag
     1201 cccgagcagc cacgccgcgg tcgcgttgat cgtcatcgac gtgttcatct cgccgggagg
     1261 gatgccgtcg aggagggtgc gcatgtggcc gaggtgcgcc accgggacgc cgacctttcc
     1321 gacctcgccc cgggccagca cgtggtcggg gtcgtagccg gtctgggtgg gcaggtcgaa
     1381 cgcgatcgac aggccggtct gccccttggc caggttggtg cggtacagct cgttggaggc
     1441 ccgggcggtc gagtggcccg aataggtccg catcaaccac gggcggtcgc gctggggcag
     1501 gtggacgacg ggatccggac cgcccggacg gggctcagca gtcatgctcc attgtcggcc
     1561 gtgccgaccc cgatccgcca gacgttcccg cggcgccggg gacggtgcgc tcggaccgac
     1621 agcggtccga ccgcggggat cgggctctgc gcgataccgg gggacccgac gagggcggga
     1681 gggaggggag cgggccggat ccggcggcgg atcgacgacc ccacacagcg gggcggagcg
     1741 agggccacaa ggcgccagca cttgggaggg tgctggtggc gatgcatttg gagggagatt
     1801 ggtgtccctc gctcctttgc cccggctgtg gggtgtcggt gggggcgatg tccaccgacg
     1861 tcattagtat cggcacaagt cccgtcggct tgaacagatt ctggccggat aatcccatta
     1921 ttttctgaga ggtctccgcg gcgtcatgac cccggaccgg accgtgaggc acccgtcatg
     1981 atcgctctgc tgctcgccgc cgatgacggg agcggcgcgc tgttcctggg cgaggacttc
     2041 tttccgtgga tgatcctcgc cttcggtgcc gccatggtcg tcggcaacat cctcgccctg
     2101 gtccgtcctg ccgcggacgg tcagcgggct ccggcgggac gggcggcggc gatgatcgtg
     2161 gtcggtgcga tcgccgcggt gtggggcgcg gtcagcctgt tgagctgacc gcaatcgccg
     2221 acggtcggtc agtggccccg gtcggtcagt ggccccggtc ggtcagtggc cccgtcggac
     2281 ccactcctcg aggttcgggg cctcggcccc gatggtcgtc gactcgccgt ggccggtgtg
     2341 cacgacggtc tcgggcggca gcgtgaggag ctgctcgcgg atcgacgtga tgatcgtcgg
     2401 gaagtccgag aagctgcgcc cggtggctcc ggggccaccg cagaacagcg tgtcgccgga
     2461 gaacacgtgc ccacccgaga cgtcgtggaa gctgcagcag ccgggcgagt ggcccggggt
     2521 gctcagcacc gcgagttcgg cgggcccagc cctcagcacg tcgcccgcgg ccaggtctcc
     2581 gtcgggatcc gcgcccgggt gcactgcgtc ccacagcatc cggtcggcgg ggtgcaacag
     2641 gaccggagcg tcgaccagct cccgcagcgc caccgccgcg ttgatgtggt cgttgtgacc
     2701 gtgggtgagc gcgatggcgg tgacccgccg cccgttcacc gcctcggcga tcacgtcggc
     2761 gtcgtgggcg gcgtcgacga tcagcaccga gcggtcgtcg ccgacgagcc agatgttgtt
     2821 ggtcacctcc cactcgccgc cgtcgagggc gaagaccccg tcggtgcgca ccagttcgat
     2881 cgtcatgggt tcaccctcct ccggacgtgg cgcacccgag cgcggcggcc tgggcgagct
     2941 tcgtgatccc gtcgacgtcg ttgttgagca ccatgtcgtc gaactcggct tcgccgacct
     3001 tctcgatcat cccgtcgacc atgcaggtcg ccatctcctt cgggatcccc gaccccgtca
     3061 gctgctcgac cagcatgtcg cgggtgccgc cgaacccgcc gccggtgctc tgggccgggc
     3121 accgggtgcc gacggcgggc agggtgaggt cgaccaggta gtcgaccacg gcgtcctcga
     3181 tgcagggacc gccccggagg aacgcggtgt ggccgtcgcc ctcgtaggtc agcagcacgc
     3241 tgcccgtgag tgcagcggac atctcctggg cccactcgta gggtgtggcc gggtcaccga
     3301 cggtgccgat gacgaggatg ggcggtgcgc cgaccgcgga cggcgccggg gtgggctccg
     3361 ccggggcggg ccagtcgttg cagccggtga ggccggcgcc gagcagaccc ccgaaggtcg
     3421 gtgccgcggc acggatcttc tcctcctcga cgcgggcctg ctcctcggtg aggtggtcgt
     3481 tctggtccgc gcagttgacc atggtctgcg cgtcgctcga gttgtcgtag ctgccgtcgg
     3541 cctgtcgccc ggtctggcgg tccaccaacg ccaggatcgt cttcgcaccc ccggagcgga
     3601 tgttcttgat cgccttcgcg gtcggacccc acgtcgaggt gtcgtagagc gcggtcgcga
     3661 ggccgtagtc gaacaggtcc gacgtcagcg tgcgggtgcc cgcgccggtc ttcacctcga
     3721 ccggcttctc ctcgagggac cggcgggtcg actcgatcgc cgccgcggtg tcgggggcga
     3781 gggcgcagga cttgtccgca tcgcaggtgg tgacgaagtt cccgagcgtg cgctcgaacc
     3841 ccgtcgcctg gaccatggcc tggtcggcca ccgatgccga cgggctgacc gagccgtcga
     3901 ggaccatcgc ccggatgttc gcggggaact tcgccgcata ggtcgccccg atggcggtgc
     3961 cgtacgagta gccgaggtag ttgagcttct cgtcgccgac cgcggcgcgg atgcggtcca
     4021 tgtccgcggc cacgtcagcg gtgctcatgt gcgtgaccag gtcggggttg cgggtctcgc
     4081 accccttccg cagctcggcc tgctgggcct cggagtgggc ccgttcggcg tcgtcgtcgg
     4141 gagacaggtc accttcgagc tgctcgtcct tctggtcgtc gtccaggcag cggaccggat
     4201 cgctctcccc gacgccgcgc gggtcgaagc tgaccaggtc gaaccggtcg gtcagcgacg
     4261 acgggaacgc catcgacgcg ttggcgagga actcgagacc cgaaccgccc ggcccgccgg
     4321 ggttcatcac gagggacccg atccgctcgt cggcggaccc cgtcgacttc ttgcgcgcca
     4381 acgccaggtc gagggtcggg ccgtcgggcg tcgaggggtc gatcgggact gccagggtgg
     4441 cgcactgcag gccggcctgc gacgtcagct tgccccggca gggggcccag tccagcgacg
     4501 tcgccggggc ggcggacccg cccgaggtgg cgggtcgcgg gtcggactcc atgcgggcat
     4561 cgtcggaacg ctgcgtgcat ccggaggtga tgagggcgac ggcggcgagc aggcacgccg
     4621 cgaccatgcg ggtcgccggc ggagccggga gggttcgggt cacgccggtc gaggctaccg
     4681 aggtgccgtc gcgacgcggt tcaggccgtc gagtcgcccg agcggcctct cagcgcgtcg
     4741 gtcggtccgt cccccgctgc cgccccgcgt gctcggggct ccgccgaggt cacgggacgg
     4801 ggacgggtgc gttcgcggcc gggtcctcgg gatcgccggg ttcgcggacg gtgtagatga
     4861 ggggccacgt cagggcggtg gtggccgcct tgagcccgac gttggtggcc atgatctgca
     4921 ccagggcggc agtcgggacg tcgccggcga aggcgatgat cgcgaacagg acggagtcgg
     4981 tcgggatcga caccgcgttc gacgccacga cccgtgccca ctggtggcgg ggcccgagcc
     5041 gttccatcac ccggtggtac acctcggtgt cgatcagctc ggccacgacc tgggccacga
     5101 tcgaggcgac gacgatgcgc gcgacgggga tgagcaggtt cccgaactcg gtctgtgggc
     5161 cgaccgtctg gtccgcgggc aggttcgcgg tgacccagaa ggcggcgacc atcaccaggt
     5221 tgagcgccgc tccggtcacg atcgtcaccc gcgccgcggt ccgcccaccg gccttgtgca
     5281 ccacgtcgcg cagggtgaac gtcaacgggt aggtgaacgt gccggcgtcg atcgcccacg
     5341 gccccaggag cacgatgcgc accgacatga cgttggccat gagcgacacg ccgatgtagg
     5401 cgccgaccgc gaccagcagc acgttctggc ggtgtcgggc gtggcggtcg gccaggtcga
     5461 gcacgggcac gggtccgaac gctagagccc cggcacggac aggtcccgct aagagcggcc
     5521 taccatccgc tccgtgccgg acgtcgacga tgccccgccg gaccgcggca ccacccggga
     5581 ccgcccggca ccctcgcccc cgtcggtcta tccgcccgat gaccacgtac tgcgcgacct
     5641 ccccttcgag atggaggaga ccggtccccg ctcggcggtg tcccatctga ggctcacgcc
     5701 gaaccactgc gaccacggac gcgtggaccc ggccacgctg ctcgtgctca ccgacgtgct
     5761 gggcggattc ctggtcggcc gggtgatcgc cccggactgg atggcgaccg ctcagctcgc
     5821 cctgcacatg ttcgaacacc cgggcgtcgg tgaactgcgg atcggagcga ccgtcgtccg
     5881 ggcgggccgc agcacggtcg tcgtgtccgc cgacatcgct gtcggcccgg cccggcctgc
     5941 cggccacggg gtgtcgggcg ccggatcgga tggtccacgc cgcgtcggag cagccgagct
     6001 gaccttcgtc cggctgcccc gacgcgacac gaccctggac ctgccgaccg acggtccctc
     6061 agccggtcga cgcctgtcac tggccgtccc cggatcgggc ctgaccgccc cgctccgccg
     6121 gtcgatcggc gttgacgagc cggggggctc cgtcggtctg gaggtgaccc catacgtccg
     6181 caacagcttc ggagccgtca acggcggcgt ggtcgccgct ctggcggccg ctgccgccga
     6241 gggagctgcc gccgaacgga ccggggtccc ggtccgggtc gcggacgtcg ccgtcaccta
     6301 cctggctcag gcccgtgtcg gtccggtcga ggcgtcggcg tcggtgctgc gcgtcgacgg
     6361 cccggtcgcc ctggtccgcg tcgaggtccg tgacccgggt ctgcccgacg acgacggccg
     6421 accccgggtc gccgtgaccg cccacgtcca ctgcgtgacg gacgcggacg cggggtccgg
     6481 gaggggttcg ctcagggatt catgacccgg gcggtgcagc cggtgcccag ggtgcggtcg
     6541 aaggccccgg tcatgaaccg ctccatcggt gatccggtga atgtgagatc cgcggcggcc
     6601 ttgccgctgc cggccgccga gatctgaccg gggatcggaa ggtgacccgc cggactggcc
     6661 gaaccgtcct tgcggtacag cgcgagctcg aggtggccac ggtctccggt cgccgggcag
     6721 tagccgtcgt cggcggtccg cagcgactcg tacagggcga cgctcatgca gtcggcggct
     6781 gcgggcacgt ccgcgtccgg cgtcccgtcg gctggtcggc agtggatgac ctggtcgtcc
     6841 atcccctggc cgatgagcat gggcaccggc gcgccgtcct gctcggggac cttcgggaac
     6901 gggttgaccc cgtcgggtcc gggcaggttc cagcggatcc aacggcacca gtcgcccagg
     6961 ccgccgccgt cgctgcgctc cgggtcgcgc gtcgcgcacg cccggtcgac gccgcccttg
     7021 aagaactcgc ccggggagta ggcgtcgggc aggttccaca gctcggggac cagcatcttg
     7081 tgacgggcgg cgtcgcggta cggcgccgcg atcgactgga ccttcctcgc gtcggtgacc
     7141 gtgcacagtg cggccagctg cgcgacggtc tcggcgccct cggcggtggt gacggcccga
     7201 aggtcgagca ctccggcgtc gcgggggaac gccggagtgg tcgcgtcatc tgcgggggtg
     7261 ccccgtcccg acagcaccgc ccacgaaccg aagatgtagg agaacagcgg aggagcgatc
     7321 tgcagctcga gggcggggag gggcaggccc agcatctcga tcgtgtggtg catctcccag
     7381 tcggccaggc cgttgcccgc ctcgatgccc tgacggtcgg gctggacgat gaagttggcg
     7441 gccggcgcca acgcggccac ccccgccagc cgcagggtgg cggcgtcgtg gttgggcgcg
     7501 gcctcctggt aggagtccat catctggccg gcccagacgg cggcgtggcc gccctgcgag
     7561 tggccccagg tgatcgtgtc gtaggcgtcg ggcgcctcgc cggggtgctg ctggtccatc
     7621 agctggctcg tcgcccgggc tgcgtcgatg acgttcgccg cggcgatgcg gccgaccacg
     7681 taggtgtcgt tgggctggta gtccgcagcc gacaccaccc aaccccggtc gagcgccgtc
     7741 tgcagcgccc cgttcgccgg gtcgcccttg cgggcaccga acccgctgcc ccacgagatg
     7801 gcgttgatgc cgtcggacat cttcccccac agcgacgtgt cgggagcgag cgacggcagg
     7861 cacgcctgct gcagcccgga ggtgccgtgg gcccacgcca gcatccgccc gaccggaccc
     7921 gagtcgacgg tgaaggtgga cgggcccttc gccggcgcgg ccgccatccc gcacacgatc
     7981 tgcaggtcgt gctcgtcgag accggtcgac acgtaggaga tccgccacgc gtgggcgtcg
     8041 gtcgggaagc ccaccgacga cttgtactgg ccgtcgagct cgtaggaggc gatcaggtct
     8101 ccggggcgtg acccgggcgg cgggtcctgg cccacgccgt tcggggtctg cacgcacggc
     8161 ggtgccgcca ccggcagcgg caccgtcgtc gtggccccgt cggccggcgg cagctccgac
     8221 gggagtcctg tgacgtcggg gtcccgagtg cacgcggcca ggccgacggc cagcgtggcg
     8281 gtcacgagca gggcggcgac gacgcgtacg tggtggcgca tggggctcat catgccgtgc
     8341 cgtgcgattt ccatgactct ccgcgccacc agtggagcgg agagtcatgg aaatcacggg
     8401 ttgggcgtca gttgcccgcg accaggacga tcttgccgaa cttcccgccg gagcggaacc
     8461 gctcgtaggc ctccgccgcg gccgacatcg ggaaggtcgc cgcgaccggg acgtgtatcc
     8521 gaccccgggt gaccagggga agcacgtggc gccggacggc gtcggccgcc gcggccttct
     8581 gctcgagcgg acgtgcccgc agcgtcgagc cgtggatgcg gccccggacc gccatcaggt
     8641 cgagcaggct gatctccgcc ttgggtccgg cgccgacgcc gatcaccgtg atgcgcccgc
     8701 cgatcgacag ggcccggacg tcgtcgcgca ggttgggtcc accgatcagc tcgagcacca
     8761 cgtcgaacgg tccgtgggcg gcgaagcgct cgggtgcgac cacggtcgcc ccgagctcgg
     8821 cgacgcggtc gcgcaggtcg gggttccgca ccgtcgccac cacgtgggcg ccggtggcga
     8881 ctcccagctg cacggcggcg accccgacgc ccccggccgc gccgtgcacg cacagcgtct
     8941 cgccgacggc gagtccggcc tgggtgaaca cggcgtcgtg cgcggtggtg aacgcctcgg
     9001 ggaatcctcc ggccccggcc cagtcgaggg tgtcgggcac ggggagcgcg cagctctcgt
     9061 gcaccaccgc cctctcggcc tgggcgccgc cgccgaccac ggccatcacc cggtcgccga
     9121 cggcgtagcg ctggacgccg gttccgaccg cgagcacctc gccggcgagc tcgaggccgg
     9181 ggatgtcggc cggtgacccg gccggggcgg ggtagtggcc tgcgagctgc aacaggtcac
     9241 ccgcgttcag ccccgccgct cggacggcga ccagcagctc tccggcctcc ggctcggggt
     9301 cggggtgctc ggtccactgc aggttcccgt cggtgatcgt cacggcgcgc atcgtccgat
     9361 cgtcgcgcac gggacgtcgc ccggggtccg gaacggagat ctggccggac tgcacggtcc
     9421 gagccggtcg accccgtgcg tcagctgccg gagatggtgc agccgtcgcc gcggtaggtg
     9481 ctgctcatcc gagggaagac agcgcggagg taccactgcg cgcggcgctc ggaccgggga
     9541 cgggacgctc ccagcgcctc ggcgacccgg gcggagttct gcacggccca gcactcggtg
     9601 gcggcctcgt tcgcctcacc gcggacgtgg ccggcctcgt gggtcagcac gtgaacggcc
     9661 gtgtaggcgt cgtcggtgtc gtcctcgggc cacgacgcgg ccgcggtgca gatcttgcgg
     9721 tcgaggtggg cgacgtcgtc catgctgccg tcgtcgcggg catcgacccg cccggcgccg
     9781 cgggtcggca tccactcgcc gaagaacagc tcctgacagt cgacgtggac ctgcacaccg
     9841 gcgacagcgc tggccgcctg ggagagccgc gcctccatgc gggcgtcgct ccaccagacc
     9901 cagccgcacg ccagcgcgat cagtccggcg agcgacccga cgaacttggt cgaacggcca
     9961 cgcgcggcgg cgaccatcgc cgcgacgaac gcggcggcga accccagggc cagcaccgcc
    10021 cacaccgtca tggcccgatg gtgccacgct ccggtgaccg cagagcccat cgcgtcgatc
    10081 cgccgcgatg tcctgagttg ctcggccatg atgtcggcgg acgaacacca ctggagacga
    10141 acgacggtga acccgatcga ggaggacgac cgatgacacc ccaggaggtc gtggcgggct
    10201 tcatcgccgc catcgagtcc cgtgacgtcg acgctgccct ggcgctcctc gccgaggacg
    10261 tgtcctacga gaacatgccg atggacccca tcgtcggccg ggagatgacg ggggtggtgc
    10321 tgcactccta cctggatctc tccaccgagg tcgagtggcg gatcgtgcgc gaggtcctcg
    10381 acggcgacgt cgtcatcaac gagagggtcg accggttcca catcggcgac ggctggttgg
    10441 agctcccggt ggccgggttc tgggaggtcc gcgacgggct gatcacgctg tggcgcgact
    10501 acttcgacat gtccgcctac acctcgcagc tcgacgcgct cgtcggcgac ggctgaatgc
    10561 ggtcgtcgct gcgacaccct cgacgtccat ccggcgtcgt atcgccgggg cgcaggttcc
    10621 gccgcggagc ggttgctgcg gcggtcggga tcctcgccat cgtcgctgcc gtcgccgcgt
    10681 gtggcggggg cgacgacgtc gccgagcggc gggcgtcgtc cgcgccgtcc tcgaccagtc
    10741 cgtcctcgac caccgcggag gtgccgatgg atccccggtc ccagctcacg gcccgattgt
    10801 cgaccgagct cgacgacccc gacggcgccc gtagcgtcgt cgccgcgatg gacgatgcag
    10861 cggtggcggc tgcagaggcg ctcgccgacg gcgacatcgc cgggagtccg tggctgtcgt
    10921 accggccctc gacggtgccg gtcgacgaga tcgactcgct gtgggtcttc tcctacggct
    10981 tccgcttcgc cgatcccgcc gccgaccccg tcgggttggg cgctcgcgtt cccggccccg
    11041 acgagttggt acccggaccg accaacgagg cgctggcaag ggctgcggcg gacgtcgtgg
    11101 tcgatcaccc ggtgcccgtc gtcgcccagt gggaggtggc ccgggagctg cagaagctgg
    11161 gcgtcgccga cgtcatctcg gtcgagcccg acacggctcc ggacggttcg gtcgtctacc
    11221 tcagcaccgc cggcgtcgcc gacaagggcc tccgccagct caccgccgcg ggcacggagc
    11281 cgggtcaggc cggcgtcctg tgcttccgtt accacgccga acgatgcgtg ctcaccgccc
    11341 gcgccgccgg gttgaccgcg gacgtccccg agggggcgga cctgccgtcg aagttcgacc
    11401 cgaagtcggg tcaggactgg acgcggtccc tcgagacgtg gatccccgtc gacctggccg
    11461 gccgaaccgt cctcaaggcg ggctgaccct gccgtccgac cagaactggg gtcggaatcg
    11521 acaggtggcg tcgattccaa ccccggttct catggcggag cgggtgacga gaatcgaact
    11581 cgcgtattca gcttgggaag ctgatcagag ggggtgcgct gaccagtcgg ttcgcgtgat
    11641 cgggctggtc aggccgtact tcgcgtgtca tgccgtatcg ctgcggcgcc cctgatccca
    11701 ctccgtaggg tcacgctagg gtcacgccgc cgggcctcac gtactcagcc ttccgagccg
    11761 atgtggcccg gatcagcgct cgccgaggga gtcgaggaac gccaccgcgg cgtagctgat
    11821 ctcctcggcg gtgatgacct ggtagcgctc gtcgttcgag atgaggagca ccgaccgggc
    11881 ccgggccgcg ccggcaacga gctggtcgcg gacggtcgtg gccaccacgc ggatgaggct
    11941 cgggttcgcg cctgcgaggt cggcggccgc gtcgccgccg aggtggagga gcaggttgta
    12001 gatgtcccag aggtcgccag gtcgcttgtc gcggccggcg acgcgacgcc ggtccaggta
    12061 ggcgtggagc ttggtggcga ggagcgcgcc agtggtggcc acgggaaccg tcgcctctgt
    12121 ccgtgtctcg gcggccagga gccgcacggg ggtcgcggcg gccagcgcat gggcgtgcgc
    12181 ggcgacgtag agcaggtcct tgtcgtcgag gtgcgacacg tcggcgtcgt cgaccggttc
    12241 gacgtcctgg aactgcacct ccgcaccggc gatcgtgatg gtgtgcggct cggctgcgtc
    12301 gtaggtggcg tcgccgcggg cgcgcaggac ctcgagagcc gtcggtgtgt agcgctggtc
    12361 ggtgacggcg tcgaggtcgg cggtcgctcg cacggctcgg cccaggcgga cggtgacggc
    12421 gacgccgccg atcaccgcga agggcgggat gtcctcggct tcgagcgcgg ctgccgctcg
    12481 cacgagcggc accatggccg ggccggggag gacgacggtc gggacgtcag cgctcatgcc
    12541 acacggcctc gccgtcgggt cgccactcgg cgagcacctg gagcgacctc gcgtcgcccg
    12601 ccgccgccag gtcgagggcg gcggccaccg ggtggaccag gagccagccg cgatggcgcc
    12661 gttcacccga tgccggatcg gggggcgccg tcacctggtg gacggccgga acggcgacgg
    12721 atgcggctgc cgccagggga tcggaggatc cgtagcgtcg gacggcgatc gacagcagca
    12781 caggaccggg gacgtagagg tcgatcggcc cgtccgcagc ggtgacgatc ggcgcgccga
    12841 gctcgatcgc ggcctgggcg cccccgaggc gccaggtcgg ttcggtcggg tcgtgtgctc
    12901 ggtcccagtc tttcgggtcg gggacgctgg cgagccatcg ccggtccgta ggcgcccatt
    12961 cgcgggcgag ggcccagaac agctcgggca gcaccggttc tccgtcctcg gtgagcagcc
    13021 cggcgccgat gagggcccgt cgggcgtcgc tggtggagga gcgggcgagg tcgaactggt
    13081 cgaacgaggt cgtggtcaga accgggcgcg gatcttcgtc gtaccgacgc cggaggagtt
    13141 cgtaggccac gactcgtccg gcccgcccga cgaccccgac aggcttcggc cgctcgcctg
    13201 gttccacggc atccacggtg gtgtcgatcc ggatgccggg agcacgcagg aacaggctcc
    13261 ccgtcgcgag gtcgaggtag ccccagcctg cttccttgag cgcagcctgg gccggctggt
    13321 tgatccggtc ggcgacgacc aggatcgact tgtccccggt cggcacgccc ttccgcttca
    13381 gagcctcgac gcggtcgacc gttgcgtagg ccattgtctt cacctcgatg gtcacccggt
    13441 gaccatccag ctcgatctcg aggtcgtccg tggagttgct cgcgggctgc acgcctccca
    13501 ccgcctcgac gagcgcggcc atggcctgct cgcgcaggct ccgatcaaga acgtcggtcg
    13561 acctccggcc catgccgggg agtgtagccg gttccggtat ccgggtatac ccggataccg
    13621 gaaccaggat agatcaaccg gttcgggagg tccgagaagg gccgtcgtgc ctattggcac
    13681 ccggcgttga atgacacttg tgtaattcgg tcgcggtcgc ccccgaagcc ttgcgccgct
    13741 tgacatcgcg aactctcaag cctgtttaga gcagcttgag agcactgcct gctgccatct
    13801 gaagacgcct cgcgtggcag ccgactcggc gctcgccgtc caggcccgcc tccaccgcga
    13861 cgctggtctc gaacccccgg acccgatcgc cgcgacgcgg gctgcgctcg ccgaccagcg
    13921 gggcgccgat gcggcgctga actcgcctga cgccccgacc gtggtcgaac cggagccgaa
    13981 gcccctgatc gcccccaccg cgccgccgcc gacccgctcg accggtccgt cgctcggtct
    14041 gtagcccggc gggtaccctg gatcttgcgt cgcccggccg cacatgacgg gccttctcca
    14101 ccggcagcca tcgctgccct ggcatctgga gcaggccatg ccacgcgaac gacgcgcagg
    14161 aggcggaatc gaccgcctcc cctcgggccg ctatcgagtc cgcatcgtca cccccgacgg
    14221 ccgacgcctc agcctcggca cccacgcca
//
LOCUS       DAOQOD010000075        14232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1517_length_14232_cov_12.474642, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000075 DAOQOD010000000
VERSION     DAOQOD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14232
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_14232_cov_12.474642"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..528)
                     /locus_tag="PLZ72_08815"
     CDS             complement(118..528)
                     /locus_tag="PLZ72_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA36436.1"
                     /translation="MLVVDTGVLVAAADLSDPAHEPCAALLASPAGPIVTTGLVIAEA
                     SYLISRELGAGPEALLFQAIAQGEVHVADLVQSDWVRIHQLVEDYRDLPLGGTDASVI
                     ALAERLDQSTVATLDHRHFGVVRPTHRDAFDLLP"
     gene            complement(531..758)
                     /locus_tag="PLZ72_08820"
     CDS             complement(531..758)
                     /locus_tag="PLZ72_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36437.1"
                     /translation="MTITVDLPDEALARLRSEAERRGISFDELLVEFAGTLPSDSAES
                     KPKLAFIGSGASKDGISDKVDELLADGFGRS"
     gene            959..1243
                     /locus_tag="PLZ72_08825"
     CDS             959..1243
                     /locus_tag="PLZ72_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36438.1"
                     /translation="MSPSPASRSTSDKVAAQGRVRLGAWMIIAGTLGTVLAVLTYIDV
                     WNVSDTKPTAWAVVAALCAVLSLGGFVVLWAGLTAGAKAAAAAGRTDEPA"
     gene            complement(1296..1658)
                     /gene="rsfS"
                     /locus_tag="PLZ72_08830"
     CDS             complement(1296..1658)
                     /gene="rsfS"
                     /locus_tag="PLZ72_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNA36439.1"
                     /translation="MTAAAPDESRRTALIAADAAEDKLATDVVVLHVGPVVGLCEYFV
                     LATGSNDRQVKAIVDAVEERVAEEIGERPRAVEGADARRWVLLDYGDVVVHVFLAEER
                     EYYRLERLYSDAEVLERP"
     gene            complement(1655..2965)
                     /locus_tag="PLZ72_08835"
     CDS             complement(1655..2965)
                     /locus_tag="PLZ72_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNA36440.1"
                     /translation="MTATETDPVAEVAPVVRRRRSEAPSTGGRRGRQGRRSGRAGGDG
                     SRTVWWQLASTAAFAVLIVALALLGYQASRLITGGGTDKVTDPAAPGYVAEVRPTPVD
                     LVAVTTDDGVLAGVLIVSSAGAKTGGTVSALPATASAGPGEKGTFPLLLDDFAAGGLD
                     QLRTSLGVGLTFGFTSAEQISARDLTTLAGVAGPITVNNVDNLIDAPDKATIRAGRTG
                     ETVRYRAGEVVLEPADVAGFLAFAGVGEAPDRQMLRHLTVWEQLLTALKGKDLSALAG
                     EGSAEAGGGIGAVSDLLGDLGAGEVTYEPVPVAPQPIPGTYFTAMVPDAAALPTYVAR
                     VVPFPTSAVPGQRARVELLNGTTDKDAALLVAPKVVAAGGEITLLGNADSFDVTATQV
                     HYPVPEAREAAEEIASALGVTATAAPASSGGVDVSVIVGSDRTS"
     gene            complement(2971..3585)
                     /gene="nadD"
                     /locus_tag="PLZ72_08840"
     CDS             complement(2971..3585)
                     /gene="nadD"
                     /locus_tag="PLZ72_08840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNA36441.1"
                     /translation="MAAGSAPGAGVGPGRRIGVFGGTFDPPHVGHLVPALFVAEALAL
                     DVVLLVVANVPWQKVGSRPISPARDRLDMVRAAVDGVDVLEASDLEIRRGGDTFTVDT
                     IAELRSQGPDDDLVLILGSDAASGLDTWERPDELRRTCRLAVVERPGEPVSVPDGFRH
                     VTVPVPQLDVSSTVLRDRAAAGLSLRYLVPDGAVAILERRRLYR"
     gene            3749..4576
                     /locus_tag="PLZ72_08845"
     CDS             3749..4576
                     /locus_tag="PLZ72_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA36442.1"
                     /translation="MSDSVGMYLNEIGAVALLSAEEERELSRTIELGAAARRRKAAGE
                     RGRDLDKQIAAAAAAKDRFIRANLRLVVSIARRYPLPPGMELLDLVQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQAIGRALDQKASLVRLPGDRSAALRGALRAVSGDGDELD
                     DENARLHRLTTPTSLDRTVGDDDSNELVDLLADDRPGPEDDLLSAEHDEWLHGLLDVL
                     DPRARSAVEARFGLSDGRRRSYREVGEDLGVTAEAARRLVKRAVNTVRDRALATASA"
     gene            4645..6015
                     /locus_tag="PLZ72_08850"
     CDS             4645..6015
                     /locus_tag="PLZ72_08850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNA36443.1"
                     /translation="MADTPVTGAERAPWTPASWRDRHAEQQPDWPDEAVLDATLKTLA
                     SYPPLVFAGEARQLTTELGRVAAGQAFLLQGGDCAESFEAFSADSIRERLKVILQMAV
                     VLTYSTGVPAVKVGRIAGQFAKPRSSAIETVDGVGLPSFRGHMVNDIAFTAAARVPEP
                     QRLIEAYHQSAATLNLLRAFTKGGFADLGRVHQWTQEFVRSSPEAVRYEQLAREIDRA
                     LRFMRACGLDPDSVPTLHEVDFYTSHEALVLGYEEALTRRDSLTGDFYDCSAHMIWIG
                     ERTRGLDDAHVEFFRGVSNPVGVKVGPTASADEVLALCQALNPDRVPGRLTLITRMGA
                     DRITDGLRPLLRAVTDAGHPVVWACDPMHGNTFTAPSGRKTRHFDAILAEIKGFFDAH
                     EAEGTWPGGVHVELTGDDVTECLGGAEELVDDDLGLRYESMCDPRLNGRQSLDLAFRV
                     AELLRG"
     gene            6191..7996
                     /locus_tag="PLZ72_08855"
     CDS             6191..7996
                     /locus_tag="PLZ72_08855"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNA36444.1"
                     /translation="MTTIEIDEQGEPSIDGIDIDPAMVADIRKFETMLARNLAGDLDD
                     DVFRVFRLANGIYGQRQGGHNQMVRIKAPYGAITPAQLDRLADISERYSRGWGHLTTR
                     QNIQFHYVQLERVPELMWDIATVGMTTREACSDTVRNVTGCHLAGACPHEVLDIQPWA
                     EATFRYFLRHPISQRLPRKFKINFSGCATDCGQAMFNDVGVIAVNRTLDDGTTEAGFR
                     VFVAGGLGANPHPALALEEFTTREELMPTIEACVRVFEQAGNRDNKLRARMKWLVDEL
                     GFDELQRRILASRRFLVASSSWPGGIPAQVVATGDAPAGTGSGPVTAVGQGTPVFLTG
                     TSELQRWDQANVVRGTARPTVSAMAWSRLGDVTAAQFRGIAAIARDLDAEVRITNRQN
                     FVLRGLREDQLPDLHARLVALDMAKPGAELARDVVACPGADTCNLAVTQSRGLANAIS
                     EALEAAGLAEVDGVRTNISGCTNSCGQHHTADIGFFGAERRAHGSSAPGYQLLLGGYV
                     GDERAEFGQKALRLPAKAAPQAVVRIVGRYSAEREYGETFSSWLGRVGGAKAVAAELK
                     DLDEFPTPEENPDFYVDYGETGPYAAVIGESECAA"
     gene            7996..8724
                     /locus_tag="PLZ72_08860"
     CDS             7996..8724
                     /locus_tag="PLZ72_08860"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNA36445.1"
                     /translation="MAITIPVNGESSGLDPAPGPFTPASFTDAELAELAAGFESAPAS
                     KIVQWAADSFGPHLALAASMTDAVLIDLAVKVAPAIEVVFIDTGYHFPETLETVERVR
                     RRYGLNLRIMTVAPHDEELWKVDPENCCSAVKVGQLDRALAGKEAWMSGLRRDEAETR
                     AGSPIVARDVRGLIKVNPLANWTAADVDDYIERHDVPVNPLIDQGYPSIGCQPCTSKV
                     APGEDPRSGRWKGRGKTECGLHLA"
     gene            complement(8801..10039)
                     /locus_tag="PLZ72_08865"
     CDS             complement(8801..10039)
                     /locus_tag="PLZ72_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36446.1"
                     /translation="MRTRAIVTLAGLSAVLLVGAACTRDGPGTDGGDASPGGGLSTPG
                     MHRIDPPPLGARHGEARALAGDQLLVWGGQTAENSMEGRTLGDGAVLDVRTGRWSRVD
                     PSPFPHGLFSPAAAFDGVEVVVVGTECDETIPAPTLGVPPECRGPAAAAWDPTDGSWR
                     RLTAPPLTTGSLGVPGFSPSVQTAGGDGRAVFVDTYPLRSIAWDRRDGSWTTVDPPVD
                     VRADAWSWPCADPSRPWVVAIGADDDPGVQLLAVLRPDGSSWTTPVRSDAPGDASSCG
                     GGRVVTSGWDGLTAVGSVIDPLTGATESAFEGGSDTPTQAAASGEWMFVRSADLTIAE
                     STTTAAGPAAGPELRTDRPTTSVRLLPDGPLVPAGTRSNLFGLTGRSASYVGTGIVMC
                     ELALPDRCRMWVPPAAATAG"
     gene            complement(10048..10896)
                     /locus_tag="PLZ72_08870"
     CDS             complement(10048..10896)
                     /locus_tag="PLZ72_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36447.1"
                     /translation="MSLTDRIKSKVERSKPRITECIIYARPLPLIADEKPPAGDDFRF
                     KCVFDPTDRHLRQLETPERLELMKPFFAHGGCVILGIHEPTDTAMAKMWLLTYTPKPS
                     DAKRGLLPIDLAKDECFLLDLWTHPDYRRQAVAFTMAYELGACVDRFYPQLRWVYGYA
                     HKDNEASRNLMELIYGMWAVQEIKEVEIGDFWVNVVPGSDTPKFGPFSKKGRHSGDGF
                     QMPGRPRSGDMYRDYHHKDGFATGIMEAEVSDGWMWPGPEWFDNDHPKGPDGKPATPE
                     ALPREI"
     gene            11077..11982
                     /locus_tag="PLZ72_08875"
     CDS             11077..11982
                     /locus_tag="PLZ72_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNA36448.1"
                     /translation="MDEPEDRPGSAPATDTAVPTRLMVLGLAHRDGSVHGSELYPVAA
                     ACGISDETVRSCMRRLIADGLFLRDGEGRDAVFRPTDAGRASLEVTHQRHLMAYAQDA
                     AGRGWDRRWRLVAFAIPESRRAARDAFRDHLRTLGGAAVQPGLYVSPHRWHGEVVEEA
                     ARLGIAEHVSTLSTEDLDVGGETNPRVLVDRLWPLAEVARSYEAFIATYRDVPERLEA
                     MRQRHERISEQDFLPGALHIAIRFSECFDADPLLPPELLPRPWPGREAREVLARCRRI
                     GVLAREDKAGPGLFRVFDDAIAHLP"
     gene            complement(12063..13571)
                     /locus_tag="PLZ72_08880"
     CDS             complement(12063..13571)
                     /locus_tag="PLZ72_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNA36449.1"
                     /translation="MSTDGTVAVAGDEDRLAGEALLDLLNGFIAELRTAGLPVSLTEN
                     LDAMEAVRHIPLDDRNAFKYALAATLVKNHAHWRAFETVFEVYFSLRGSEYSIDEDGD
                     GSGPEVELDEDLDGEQPEGAGKGDGGGGHADGMTPEELAEALYQALLKGDQARMRAMA
                     RQAVKRYAGMEPGRPVGGTYYLYRTMRNLDLDGMLERLMEQSRSDAPEELTPLEERLE
                     HDEYEHRIEQLKQEIEAEIRRRLVADRGVDAMAKTLRKPLPEDIDFMHASREEMVALR
                     KALQPLTRRLAVRLARKRRHGRKGALDFRNTVRHSLSYGGVPAEPKFRYPRPAKPEIF
                     VVADISGSVAAFARFTLHLVHAIASQFSRVRSFVFIDGIDEVTRFFEGEEDIVQAIHR
                     VNTEADVVWVDGHSDYGHAFEVFWNRWGREIGPKTTVIILGDARNNYHASQSWVIKEM
                     RQKARHVYWLNPEPRAYWDTGDSIVGEYAVHCDGAFECRNLRQLERFVDHLV"
     gene            complement(13568..>14232)
                     /locus_tag="PLZ72_08885"
     CDS             complement(13568..>14232)
                     /locus_tag="PLZ72_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA36450.1"
                     /translation="SKALYEWNYKKQLLRIQASRGESETAGWDQIEEDIFSDDFLLTR
                     PLLEAIRAEDPVVLLIDEVDRVEVETEALLLEILSDYQVSIPELGTITARQIPMVFLT
                     SNNTRELSEALKRRCLYLHVDYPDMEREKEIVLTKVPGVTETLADEIARIVRSIRQIE
                     LKKAPSVSETIDWARTLVLLGKDHVDADTAVGTVNILLKYQSDIAKAVKEIAAEPQAF
                     KR"
BASE COUNT         2102 a         5211 c         4888 g         2031 t
ORIGIN      
        1 tcccatcggc tccactccgg ggtctgtcgg cgtcccgcac gggacgtcga catccgtaga
       61 accttctcca gcactgggat gtcgtctccc gacggccgag gctgcccgga tcaccgatca
      121 gggcagcagg tcgaacgcgt cccggtgcgt tggtcgcacc acgccgaagt gacgatggtc
      181 cagagtggcc acggtcgact ggtcgagacg ctccgccaga gcgatcacgc tcgcgtcggt
      241 gccgccgagt ggcaggtccc ggtagtcctc gacgagctgg tggatccgta cccaatcgga
      301 ctgcacaaga tccgcgacgt gaacctcgcc ctgggcgatc gcctggaaga gcaaggcctc
      361 gggtccggcg ccgagctccc gactgatcag gtaggacgcc tcggcgatga ccagcccggt
      421 cgtgacgatc gggcccgccg ggctggcgag cagcgccgca cacggctcgt gcgccgggtc
      481 tgataggtcg gcagcggcga ccaggacgcc ggtgtcgacg acgagcaccg tcaggatcgg
      541 ccgaagccgt ccgcgagcag ctcgtcgacc ttgtccgaga tgccgtcttt cgatgcaccc
      601 gagccgatga acgccagctt gggcttcgac tccgcgctgt cggacggcag cgtcccagcg
      661 aactcgacga gcagctcgtc gaacgagatc ccccgacgct ccgcctctga gcgcaaccgc
      721 gcgagcgctt cgtccgggag gtcgacggtg atggtcacct cgtcaggata cgccaaggtg
      781 gtcgcgttgg tccctcgtac tcgacgagcg ccaccgaact tgtaggccac aggtcgccgc
      841 tgcgaccacg agctacaagt ccgacgagcg ccaccgaact tgtaggccac aggtcgccac
      901 agcgaccgag agctacaagt tcgacaggcg acggtccggc tgcgatagaa atcggtccgt
      961 gagcccctcc cccgccagcc ggtccaccag cgacaaggtc gccgcccagg gccgcgtgcg
     1021 cctcggggcg tggatgatca tcgccgggac gctcggcacc gtgctcgccg tgctgaccta
     1081 catcgacgtg tggaacgtgt cggacaccaa gccgaccgca tgggcggtcg tcgcagcgct
     1141 gtgcgcagtg ctgtcgctcg gggggttcgt cgtgctgtgg gccgggctga ccgccggcgc
     1201 caaggccgct gcagccgcgg gccgcaccga cgagccggcc tgaccgaccg gaccaccgac
     1261 gggaccaccg acgggaccac cgacgggagc gggcctcagg gccgctcgag cacctcggcg
     1321 tcggagtaca gccgctcgag ccggtagtac tcgcgctcct cggccaggaa cacgtggacg
     1381 accacgtctc cgtagtcgag cagcacccat cggcgggcat cggcgccctc gaccgcccgg
     1441 gggcgttcgc cgatctcttc ggccacccgc tcctcgaccg cgtcgacgat cgccttcacc
     1501 tgccggtcgt tggacccggt ggcgagcacg aagtactcgc acagaccgac caccgggccg
     1561 acgtgcagca cgacgacgtc ggtggcgagc ttgtcctcag ccgcgtcggc cgcgatcaga
     1621 gcggtgcgcc gcgactcgtc gggggcggcc gcggtcacga cgtgcggtcc gaaccgacga
     1681 tcacgctcac gtcgacaccc cccgagctcg ccggagcggc ggtggccgtg acccccaacg
     1741 cggacgcgat ctcctctgcg gcctcgcggg cctcgggcac ggggtagtgg acctgagtgg
     1801 cggtgacgtc gaacgagtcg gcgttgccga gcagggtgat ctcgccaccg gccgcgacca
     1861 ccttgggggc gaccagcagc gcggcgtcct tgtcggtcgt gccgttgagg agctcgacgc
     1921 gggcccgctg gccggggacc gcagaggtcg ggaagggcac gacgcgggcc acataggtgg
     1981 gcagcgcggc ggcgtcgggc accatcgcgg tgaagtaggt gcccgggatc ggttggggtg
     2041 ccacgggaac gggctcgtag gtgacctctc cggcgccgag gtcgcccaac aggtccgaca
     2101 ccgcgccgat gccccctccg gcctcggccg atccctcgcc ggccagggcg ctcaggtcct
     2161 tgcccttcaa cgcggtcagg agctgctccc acacggtgag gtggcgcagc atctggcggt
     2221 ccggggcctc acccacgccg gcgaaggcga ggaaccccgc cacatcggcg ggctcgagca
     2281 cgacctcacc ggcccggtag cgcaccgtct cgccggtccg gccggcccgg atggtcgcct
     2341 tgtcgggcgc gtcgatcagg ttgtcgacgt tgttgaccgt gatcggtccg gccactccgg
     2401 ccagggtcgt caggtcgcgg gcggagatct gctccgccga ggtgaacccg aacgtgagcc
     2461 cgactccgag cgaggtgcgc agctggtcca ggccgccggc ggcgaagtcg tcgagcagca
     2521 gcgggaacgt gcccttctcc cccggtccgg ccgaggccgt ggccgggagc gccgagacgg
     2581 tgccgccggt cttggccccc gccgaggaca cgatcagcac accggccagc accccgtcgt
     2641 cggtggtgac cgcgaccagg tcgaccgggg tcggccgcac ctcggcgacg tagcccggag
     2701 ccgccgggtc ggtgaccttg tcggtcccgc cgccggtgat cagtcgactg gcctggtagc
     2761 cgagcagggc gagcgccacg atcagaaccg cgaacgctgc tgtcgaggcg agctgccacc
     2821 acaccgtgcg ggaaccgtcg cctccggccc gcccggagcg tcgaccctgg cgaccccggc
     2881 gaccgccggt cgacggcgct tcgctgcgcc gacgtcgaac gacgggcgcg acctcggcca
     2941 cgggatcggt ctcggtggcc gtcatggcgc tcaacgatag aggcggcggc gttccaggat
     3001 ggcgacggcc ccgtcgggca ccaggtagcg caacgagagc ccggcggcgg cccggtcgcg
     3061 caggacggtc gacgacacgt cgagctgcgg taccggcacc gtcacgtggc ggaacccgtc
     3121 ggggaccgag acgggttcgc cgggacgttc gaccacggcc agccgacacg tccggcgcag
     3181 ctcgtcgggc cgttcccacg tgtcgaggcc ggacgcggcg tcggatccca ggatgagcac
     3241 caggtcgtcg tcggggccct gggagcgcag ctcggcgatc gtgtcgacgg tgaacgtgtc
     3301 gcctcctcga cggatctcga ggtcgctcgc ctccagcacg tcgacgccgt cgaccgcggc
     3361 tcggaccatg tcgaggcggt cccgggccgg gctgatcgga cgcgacccga ccttctgcca
     3421 cggcacgttg gcgaccacca gcagcacgac gtcgagggcg agggcctcgg ccacgaacag
     3481 ggcgggcacc aggtgcccga cgtgaggggg gtcgaaggtg ccgccgaaga ccccgatccg
     3541 acgacccggg ccgacgccgg cccccggagc cgaacccgcg gccatcggcg gatggtatcg
     3601 gccgattccc aaaagcgtcg cgaatgggcc ggacgacctc tcaactcgtc gccggacgcg
     3661 ccgatcaaca tcaggtcggg acttcgcagc cgaacctcga ccggcgccgc gccgtttccc
     3721 agagctgaca ccagagggat ggaatctgat gagcgattcc gtagggatgt acctgaacga
     3781 gatcggtgcc gtggcgctgc tgagcgccga ggaggagcgc gagctctccc gcacgatcga
     3841 gctcggcgcc gcggcccggc gccgcaaggc cgccggggag cggggccggg acctcgacaa
     3901 gcagatcgcc gcggccgcgg cggccaagga ccgattcatc cgcgccaacc tccgcctggt
     3961 ggtcagcatc gcccgccgct acccgctgcc ccccggcatg gagctgctcg acctggtcca
     4021 ggaggggaac ctgggcctgg agcacgcggt ggacaagttc gactggcgca agggcttcaa
     4081 gttctccacc tacgccacgt tctggatccg ccaggcgatc ggacgtgccc tcgaccagaa
     4141 ggcgtcgctg gtgcgactcc ccggtgaccg ctccgccgcc ctgcgcggcg cgctccgggc
     4201 cgtgtcgggc gacggcgacg agctcgacga cgagaacgcc cgactgcacc gcctcaccac
     4261 gccgacgtcg ctcgaccgca ccgtcggcga cgacgactcc aacgagctgg tcgacctgct
     4321 cgccgacgac cggcccggcc ccgaggacga cctgctcagc gccgagcacg acgagtggct
     4381 ccacggtctc ctcgacgtgc tcgatccccg cgcccgcagc gcggtcgagg cccgcttcgg
     4441 cctctccgac ggacgccgcc gcagctaccg ggaggtcggc gaggacctgg gcgtcaccgc
     4501 cgaggccgcc cgccggctgg tcaagcgggc ggtcaacacc gtccgggacc gggcgctggc
     4561 caccgcgtcg gcctgagcgc cggtcgcccg gcccaccggg tttcccgctt cggccccggg
     4621 gcgggccccg gatatcctgg cccgatggcc gacacgcccg tgaccggagc cgagcgcgcc
     4681 ccgtggaccc ctgcgtcctg gcgggatcgt cacgccgagc agcaacccga ctggcccgac
     4741 gaggccgtcc tcgacgccac cctcaagacc ctggcgtcct acccgccgct ggtgttcgcg
     4801 ggtgaggccc gtcagctcac caccgagctc ggtcgggtcg cggccggcca ggcgttcctg
     4861 ctccagggcg gcgactgcgc cgagtccttc gaggcgttct cggcggactc catccgggag
     4921 cgcctcaagg tgatcctcca gatggcggtc gtgctcacgt actcgaccgg cgtgccggcg
     4981 gtgaaggtgg gtcgcatcgc cggccagttc gccaagccgc gctcctcggc gatcgagacg
     5041 gtcgacggcg tcgggctgcc cagcttccgg ggccacatgg tcaacgacat cgccttcacc
     5101 gctgcggccc gcgtccccga gccgcagcgt ctgatcgagg cgtaccacca gtccgcggcc
     5161 accctgaacc tgctgcgggc gttcaccaag ggcggcttcg ccgacctcgg acgggtccac
     5221 cagtggaccc aggagttcgt gcgctccagc cccgaggcgg ttcgctacga gcagctcgcc
     5281 cgcgagatcg accgggcgct gcggttcatg cgcgcctgtg gcctcgaccc cgactcggtg
     5341 ccgacccttc acgaggtcga cttctacacc agccacgagg cgttggtgct cggctacgag
     5401 gaggcgctca cccgccgtga ctcgctcacc ggcgacttct acgactgctc ggcgcacatg
     5461 atctggatcg gcgagcgcac ccgcggcctc gacgacgccc acgtcgagtt cttccgcggc
     5521 gtcagcaacc cggtcggcgt caaggtcggc ccgaccgcat cggccgacga ggtgctggcg
     5581 ttgtgccagg ccctcaaccc cgatcgggtt ccgggccgcc tgacgctgat cacccggatg
     5641 ggcgccgatc gcatcaccga cggcctgcgg cccctgctgc gggcggtcac cgacgccggc
     5701 cacccggtcg tgtgggcgtg cgacccgatg cacggcaaca ccttcaccgc cccgagcggc
     5761 cgcaagaccc ggcacttcga cgccatcctc gccgagatca agggcttctt cgacgcccac
     5821 gaggccgagg ggacctggcc cggcggggtg cacgtggagc tgaccggcga cgacgtcacc
     5881 gagtgcctcg gcggcgccga ggagctggtc gacgacgacc tgggcctgcg ctacgagtcg
     5941 atgtgcgatc cgcgcctcaa cggtcgccag tcgctcgatc tggcgttccg ggtcgccgag
     6001 ctcctccgcg gctgactccc ccgcccctcc tgcgggggat ctggggttcc cttcgacaca
     6061 ccgtgtcgat tccgcgcccg gttctccggg gacggcgtcc gatcgaatgt tgacccgata
     6121 ggtcatgttt gcgaaactcg accgatccgg tcaggtattc ggccgggcag cgacccgacc
     6181 ggaagccccc atgaccacga tcgagatcga cgagcagggt gaaccgtcca tcgacgggat
     6241 cgacatcgac cccgccatgg tcgccgacat ccgcaagttc gagaccatgc tggcgcgcaa
     6301 cctcgccggc gacctggacg acgacgtgtt ccgcgtcttc cgcctggcca acggcatcta
     6361 cggacagcgc cagggcggcc acaaccagat ggtgcgcatc aaggccccct acggggcgat
     6421 caccccggcg cagctggacc gcctggccga catctccgag cgctacagcc gcggctgggg
     6481 acacctgacc acccgccaga acatccagtt ccactacgtg cagctcgagc gggttcctga
     6541 gctgatgtgg gacatcgcga cggtcggcat gaccacccgc gaggcgtgct cggacaccgt
     6601 gcgcaacgtg accggctgcc acctcgccgg ggcgtgcccg cacgaggtgc tcgacatcca
     6661 gccctgggcc gaggcgacgt tccgctactt cctgcgccac ccgatcagcc aacggctccc
     6721 ccgcaagttc aagatcaact tctcgggatg cgccaccgac tgcggccagg ccatgttcaa
     6781 cgacgtcggc gtcatcgcgg tgaaccgcac cctcgacgac gggacgaccg aagcgggctt
     6841 ccgggtcttc gtcgccggcg ggctcggcgc caacccgcac cccgctctcg ccctcgagga
     6901 gttcaccacc cgcgaggagc tgatgccgac catcgaggcc tgcgtccggg tcttcgaaca
     6961 ggccggcaac cgcgacaaca agctgcgggc ccgcatgaag tggctcgtcg acgagctggg
     7021 cttcgacgag ctccagcgcc ggatcctggc gtcgcggcgg ttcctggtcg cgtcgtcgtc
     7081 ctggccgggc ggcatccccg cccaggtcgt cgccaccggc gacgcacccg ccggcaccgg
     7141 ctccggcccg gtgaccgctg tcggccaggg caccccggtg ttcctgaccg gcacctccga
     7201 gctgcagcgc tgggaccagg ccaacgtggt gcgcggcacc gccaggccca ccgtgtcggc
     7261 catggcgtgg tcacggctcg gcgacgtcac cgccgcccag ttccgcggca tcgccgccat
     7321 cgcccgcgac ctcgacgccg aggtccgcat caccaaccgt cagaacttcg tgctgcgagg
     7381 actgcgcgag gaccagctcc cggacctgca cgcccggctg gtcgccctcg acatggccaa
     7441 gcccggcgcc gaactggccc gcgacgtggt ggcctgcccc ggcgccgaca cctgcaacct
     7501 cgccgtcacc cagagccgtg gcctggccaa cgcgatcagc gaggcgctcg aggcggccgg
     7561 cctggccgag gtcgacggcg tccgcaccaa catctcgggc tgcaccaact cgtgcggcca
     7621 gcaccacacc gccgacatcg gcttcttcgg cgccgaacga cgagcccacg gatcctcggc
     7681 ccccggctac cagctgctgc tcggcggcta cgtcggtgac gagcgcgccg agttcggcca
     7741 gaaggcgctg cgtctgcccg ccaaggccgc cccccaggcg gtggtgcgca tcgtcggccg
     7801 ctactccgcc gagcgcgagt acggcgagac gttcagctcg tggctcgggc gggtcggcgg
     7861 cgccaaggcc gtcgccgccg agctgaagga cctcgacgag ttcccgactc cggaggagaa
     7921 ccccgacttc tacgtcgact acggcgagac cggcccgtac gcggcggtca tcggcgagtc
     7981 ggagtgcgcg gcctgatggc gatcaccatc cccgtcaacg gcgagtcgag cggtctggac
     8041 ccggctcccg gaccgttcac ccccgcctcg ttcaccgacg ccgagctcgc cgagctcgcc
     8101 gccggattcg agtcagcccc ggcgtcgaag atcgtgcagt gggccgccga cagcttcggt
     8161 ccgcacctgg cgttggccgc gtcgatgacc gacgccgtgc tgatcgacct ggcggtcaag
     8221 gtcgccccgg cgatcgaggt cgtcttcatc gacaccggct accacttccc cgagacgctc
     8281 gagaccgtcg agagggtccg gcgccgctac gggctgaacc tgcggatcat gaccgtcgcc
     8341 ccccacgacg aggagctctg gaaggtcgac ccggagaact gctgctcggc ggtcaaggtc
     8401 ggtcagctcg accgggcgct ggccggcaag gaggcctgga tgtcggggct gcgccgcgac
     8461 gaggccgaga cccgggccgg gtcgccgatc gtggcccgcg acgtgcgcgg cctgatcaag
     8521 gtgaaccctc tggccaactg gaccgcggcc gacgtcgacg actacatcga acgccacgac
     8581 gtgccggtca acccgctgat cgaccagggc tacccgtcga tcggctgcca accctgcacc
     8641 tcgaaggtcg ctccgggcga ggacccccgc tcgggccgct ggaagggccg cggcaagacc
     8701 gagtgcggtc tgcacctggc ctgagcgctg aattggacct gagcgctgcg ctcgacctga
     8761 ggactgcgct cgccctgggg catacgcagt cacccggcca tcacccagcc gtcgcggccg
     8821 ccggcggcac ccacatccga catcggtccg gcagtgccag ctcgcacatg acgattccgg
     8881 ttccgacgta gctggccgat cggccggtca acccgaacag gttcgaccgg gttccggccg
     8941 gaaccagcgg accgtccggc aacagacgca cgctcgtcgt gggtcggtcc gtccggagct
     9001 caggccccgc cgcgggtccc gctgcggtcg tggtggactc ggcgatcgtg agatccgccg
     9061 atcgaacgaa catccactcg ccggaggcgg cggcctgcgt cggggtgtcg gatccgccct
     9121 cgaacgcgga ttccgtcgcc ccggtcaacg ggtcgatcac cgacccgacc gcagtcagtc
     9181 cgtcccagcc cgaggtcacg acccgcccgc cgccgcagct ggacgcgtcg cccggcgcat
     9241 cggatcgaac cggtgtcgtc cacgaggaac cgtccgggcg caggacggcg agcagctgga
     9301 cgcccgggtc gtcgtctgct ccgatcgcca ccacccatgg ccgggacgga tcggcgcacg
     9361 gccacgacca ggcatcggcc cgcacgtcga ccggcgggtc gaccgtcgtc cacgaaccat
     9421 cgcgacgatc ccaggcgatg gatcgcagcg gatacgtgtc gacgaacacc gctcgcccgt
     9481 cgccaccggc ggtctggacg ctcggcgaga atccgggaac gccgagcgat cccgtggtca
     9541 gcggtggcgc agtcaaccgt cgccacgagc cgtcggtcgg gtcccacgcg gcggcagccg
     9601 gtccccggca ctccgggggg accccgaggg tgggcgcggg gatcgtctcg tcgcactcgg
     9661 tgccgaccac caccacctcg acaccgtcga acgcggcggc gggtgagaag agtccgtgcg
     9721 gaaacgggga cgggtccact cggctccacc ggccggtccg cacgtcgagg accgccccgt
     9781 cgcccagggt ccggccctcc atcgagttct ccgccgtctg tccgccccac acgagcagct
     9841 gatccccggc gagcgcacgc gcctcgccgt gacgggcacc gaggggcggc ggatcgatcc
     9901 ggtgcatccc cggagtcgac aagcccccac cgggcgacgc gtcaccgcca tcggtgccgg
     9961 gcccgtcgcg ggtgcaggcg gcgccgacca gcagaacggc cgacagcccc gccagcgtga
    10021 cgatcgcccg ggtccgcacc gagcggctca gatctcccgg gggagggctt ccggcgtggc
    10081 gggtttgccg tcggggccct tggggtggtc gttgtcgaac cactcgggac cgggccacat
    10141 ccagccgtcg gacacctcgg cctccatgat cccggtcgcg aacccgtcct tgtggtggta
    10201 atcgcggtac atgtcgccgc tgcggggccg gcccggcatc tggaacccgt cgccggagtg
    10261 gcggcccttc ttggagaacg gaccgaactt gggggtgtcg gagccgggca cgacgttgac
    10321 ccagaagtcg ccgatctcca cctccttgat ctcctgcacc gcccacatgc cgtagatcag
    10381 ctccatcagg ttgcgcgacg cctcgttgtc cttgtgggcg tagccgtaca cccatcgcag
    10441 ctgcgggtag aagcggtcta cgcaggcgcc gagctcgtag gccatcgtga aggcgacggc
    10501 ctgacggcgg tagtcgggat gggtccacag gtcgagcagg aagcactcgt ccttggccag
    10561 gtcgatcggc agcagacccc gcttggcgtc cgacggcttg ggtgtgtagg tcaggagcca
    10621 catcttggcc atggcggtgt cggtcggctc gtggataccc aggatcacgc agccgccgtg
    10681 ggcgaagaac ggcttcatca gctcgagccg ttccggcgtc tccagctggc ggaggtggcg
    10741 gtcggtcggg tcgaacacgc acttgaaccg gaagtcgtca ccggccggcg gcttctcgtc
    10801 cgcgatcagc ggcagcggcc gggcgtagat gatgcattcg gtgatccgcg gcttggaccg
    10861 ctcgaccttc gacttgatcc ggtcggtcaa cgacacgttg cgcccccctg gttctcgatg
    10921 gcgggcccgc cccgccgtcc cgtctccccg gacggtgccg gtcacggtac ccggcgcggg
    10981 cgggcccggt tgacaggatc ggacccctgg cgacacccta cggtcgtgtc ccgaacgacc
    11041 gtaggacgaa cgaccgacga tccggttgac gaaccggtgg acgaaccgga ggaccgaccg
    11101 ggaagtgcgc cggcgaccga caccgcggtt ccgacccgcc tcatggtcct gggcctggcc
    11161 caccgcgacg gctcggtgca cggatcggag ctctacccgg tcgccgccgc atgcgggatc
    11221 tccgacgaga cggtccgctc ctgcatgcgc cgcctgatcg ccgacggcct cttcctccgc
    11281 gacggcgagg gccgcgacgc ggtgttccgg cccaccgacg ccggacgggc ctcgctcgag
    11341 gtcacccacc aacggcacct gatggcgtac gcgcaggacg cggcgggccg gggttgggac
    11401 cgacgctggc gactggtcgc cttcgccatc cccgaatcgc gacgcgccgc ccgcgacgcc
    11461 ttccgggacc acctccgcac cctcggcgga gccgcggtgc aaccgggcct gtacgtctcg
    11521 ccgcaccgct ggcacggaga ggtcgtcgag gaggccgccc gcctcggcat cgccgagcac
    11581 gtcagcacgt tgagcaccga ggacctcgac gtgggcggcg agaccaaccc ccgggtgctg
    11641 gtggaccgtc tctggccgct ggccgaggtc gcccgcagct acgaggcgtt catcgccacc
    11701 taccgcgacg tccccgagcg gctcgaggcc atgcgtcagc gccacgagcg catctccgag
    11761 caggacttcc tgcccggcgc gctgcacatc gccatccgct tcagcgagtg cttcgacgcg
    11821 gatccgctgc tgccaccgga gctgctgccg cggccgtggc cgggtcgtga ggcccgtgag
    11881 gttctggccc gctgccgacg catcggggtg ctggcccgtg aggacaaggc cggacccggc
    11941 ctgttccggg ttttcgacga cgccatcgcc cacctcccct gaccctcccc gttctgttcg
    12001 ttggatcaca cccctgaggg tgtgatccaa cgaacagaac gggggtcgga ccgggtcgag
    12061 cgctagacga ggtggtcgac gaaccgctcg agctggcgca ggttgcggca ctcgaacgcc
    12121 ccgtcgcagt gcacggcgta ctctccgacg atcgagtcgc cggtgtccca gtaggcccgc
    12181 ggctcggggt tgagccagta gacgtgacgg gccttctggc gcatctcctt gatcacccag
    12241 gactgcgacg cgtggtagtt gttgcgggcg tcacccagga tgatcaccgt cgtcttgggc
    12301 ccgatctccc gaccccagcg gttccagaac acctcgaagg catggccgta gtccgagtgg
    12361 ccgtccaccc agaccacgtc ggcctcggtg ttgacccggt ggatcgcctg gacgatgtcc
    12421 tcctcaccct cgaagaaacg ggtgacctcg tcgatgccgt cgatgaacac gaagctgcgc
    12481 acccgtgaga actggctggc gatcgcatgc accaggtgca gggtgaagcg ggcgaacgcg
    12541 gccaccgacc cggagatgtc ggcgacgacg aagatctcgg gcttggccgg ccgcgggtag
    12601 cggaacttcg gctccgcggg aacaccgccg tagctgagcg agtggcgcac cgtgttgcgg
    12661 aagtcgagcg cccccttgcg gccgtgccgc cgcttgcggg ccagacgcac cgccaggcgc
    12721 cgggtcaggg gctgcagcgc cttgcgcagc gccaccatct cctcgcggct ggcgtgcatg
    12781 aagtcgatgt cctcgggcag cggcttgcgc agagtcttgg ccatggcgtc gacgccgcgg
    12841 tcggcgacca gccggcgacg gatctcggcc tcgatctcct gcttgagctg ctcgatgcgg
    12901 tgctcgtact cgtcgtgctc gaggcgctcc tcgagcgggg tcagctcctc gggcgcgtcg
    12961 gagcgggact gctccatcag ccgctcgagc atcccgtcga ggtccaggtt ccgcatggtg
    13021 cggtacaggt agtacgtgcc gccgaccgga cggccgggct ccatgccggc gtagcgcttg
    13081 accgcctgac gggccatggc ccgcatccgc gcctggtcgc ccttcaacag cgcctggtac
    13141 agcgcttcgg ccagctcctc gggggtcatc ccatcggcgt ggccaccacc gccgtcgccc
    13201 ttgccggcgc cctcgggctg ctcgccgtcg aggtcctcgt cgagctcgac ctccgggccg
    13261 ctgccgtcgc cgtcctcgtc gatcgagtac tccgagccgc gcagcgagaa gtacacctcg
    13321 aacacggtct cgaacgcccg ccagtgggcg tggttcttga ccagcgtcgc ggccagtgcg
    13381 tacttgaacg cgttgcggtc gtcgaggggg atgtggcgga ccgcctccat cgcgtcgagg
    13441 ttctcggtca ggctcaccgg cagccccgcg gtgcgcagct cggcgatgaa gccgttgagc
    13501 aggtcgagca gcgcctcgcc cgccaaccgg tcctcgtcgc cggccaccgc gaccgtgccg
    13561 tcggtgctca ccgcttgaag gcctgtggtt cggcggcgat ctccttgacc gccttggcga
    13621 tgtcggactg gtacttgagc aggatgttga cggtgccgac cgcggtatcg gcatcgacgt
    13681 ggtccttgcc caacagcacc agcgtgcggg cccagtcgat cgtctcggac accgacggcg
    13741 ccttcttcag ctcgatctgg cggatggatc ggacgatgcg ggcgatctcg tcggccaggg
    13801 tctcggtcac gcccgggacc ttggtcaaca cgatctcctt ctcgcgctcc atgtcggggt
    13861 agtcgacgtg caggtacagg caccgacgct tgagcgcctc ggacagctcc cgggtgttgt
    13921 tggaggtcag gaacaccatc gggatctgac gggcggtgat cgtgccgagc tcggggatcg
    13981 acacctggta gtcggagagg atctcgagca ggagggcctc ggtctcgacc tcgacgcggt
    14041 cgacctcgtc gatcagcagc accacgggat cctcggcgcg gatcgcctcg agcagcggac
    14101 gggtcaggag gaagtcgtcg gagaagatgt cctcctcgat ctggtcccag cccgccgtct
    14161 cgctctcgcc ccgcgacgcc tggatgcgca ggagctgctt cttgtagttc cactcgtaga
    14221 gcgccttgga ct
//
LOCUS       DAOQOD010000076        14181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1526_length_14181_cov_12.579428, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000076 DAOQOD010000000
VERSION     DAOQOD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_14181_cov_12.579428"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..419
                     /locus_tag="PLZ72_08890"
     CDS             273..419
                     /locus_tag="PLZ72_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36451.1"
                     /translation="MTGPSGQVYMLHIEAWREDEVRRTVHLSVIGNRGSGLRLSGKGL
                     WRLL"
     gene            complement(488..1630)
                     /locus_tag="PLZ72_08895"
     CDS             complement(488..1630)
                     /locus_tag="PLZ72_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNA36452.1"
                     /translation="MSSGHIVERNGSFRVMVYAGRDPVTGSKRQITRTVRGSRKDAER
                     VRNQLLVQVQNGTASPSSATFGELLDAWYDAVSPDWSPRTAVEHRRIIDSVLDPALGK
                     KKLANLRASDLDALYGSLRAGAGDRKPLSPATVRKFHTVARAALQQAVRWEWIGSNPA
                     ANASPPKGRRKEPKPPTPEDLARLLKAAEEQDPDFYVFVRLAAVLGARRGEVLGIRWA
                     DLTSDHHSVEINSSVVIGDGQVVRKETKTDRVRRVAIDDATAALLDQHRERWEDRARE
                     GGTELVDDAYVLSYEIDGSLPWRPDSATRRFTKLRDKLGLDDVRLHDLRHYVATRLIA
                     NGVDPRTVANRLGHASPSTTLSIYSHFVPEADRDAADLLGGLIDGE"
     gene            complement(1751..1826)
                     /locus_tag="PLZ72_08900"
     tRNA            complement(1751..1826)
                     /locus_tag="PLZ72_08900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Glu,seq:ctc)
     gene            complement(1888..1962)
                     /locus_tag="PLZ72_08905"
     tRNA            complement(1888..1962)
                     /locus_tag="PLZ72_08905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Gln,seq:ctg)
     gene            2124..2570
                     /locus_tag="PLZ72_08910"
     CDS             2124..2570
                     /locus_tag="PLZ72_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNA36453.1"
                     /translation="MTGATSSDEDMDLDLDWSGLEVLTYDECRYLLSIEPVGRLGFVH
                     DGSPVVLPINYVLDGGSIVFRTGRGSKLETATMGRAVCVEIDSWNALEHTGWSVVAKG
                     HAEHVADGAELERLARLPVKPWCRPDVRRNWVRVMIEELSGRRIHQ"
     gene            complement(2599..3843)
                     /locus_tag="PLZ72_08915"
     CDS             complement(2599..3843)
                     /locus_tag="PLZ72_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNA36454.1"
                     /translation="MATELGADGWLLISAALVLFMTPGLAFFYGGMDRSRNVLNMLMM
                     NFWCLLIIPIVWVVIGYSIAYGDTAGSLFGGFDAAFLGNLNLSSQSGADTLMLVAFLG
                     MFAVITPALISGAVADRMKFAAWAVFAPVWMLLVFAPVFKWVYGGWLGVRGSLDFAGG
                     TAIHVNAGIAALVAVLILGKRKGWPGEGHPPHSMPLVMIGTGILWFGWFGFNAGSALS
                     ANGVAVQAFMNTFLAAAAAGLAWAIVERIKDGHFTNLGAASGIVAGLVAITPACGYVS
                     SMSSIFIGLAAGVICCYAVHIKFRAGFDDALDVVGVHFVGGLVGSLMIGLFADADYFG
                     AEHMNGLFYGGGLELLAEQALANGVTIVYSGIVTAVILLAIKATIGLRVTEEVEATGL
                     DVAEHAETAYNTGDAFMERAGF"
     gene            4022..4276
                     /locus_tag="PLZ72_08920"
     CDS             4022..4276
                     /locus_tag="PLZ72_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNA36455.1"
                     /translation="MCRNITTLRGLEPAATPEEIEAAARQYVRKVSGVQSTSAATEVA
                     FERAVALVTDATAELLAQLPPRKVPPKNDPPLRRLAARGA"
     gene            complement(4283..5095)
                     /locus_tag="PLZ72_08925"
     CDS             complement(4283..5095)
                     /locus_tag="PLZ72_08925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36456.1"
                     /translation="MPLPSPAPDRTALVTGASSGIGTEIARELARRGQNVTLVARRAD
                     KLHELATELSALGVTAEVLAADLSDRDARAELPDRVAANGRTVSILVNNAGLSTVGPV
                     HRSDPAAELNMVEVDVVAVVDLCSRFLPGMVERRTGAVLNVASTAAFQPLPGQAGYAA
                     GKAFVLSYTRSLGGELRDTGVTATALCPGPVHTGFADAAGISAEDAESSLPSFMWESA
                     DAVARCGVDGLAAGRPVAIPGPANRVSASLVGLVPKQLLVPLLASRHPGLKD"
     gene            5179..6228
                     /locus_tag="PLZ72_08930"
     CDS             5179..6228
                     /locus_tag="PLZ72_08930"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNA36457.1"
                     /translation="MDPRPGHPEVLALDPVRPDPEQLAALADRMAAGSLVAFPTETVY
                     GLGARIDRPAALDRIFAVKGRPATDPLIVHVASIDALDGIVGRVPDRLTALARAFWPG
                     PLTVVLDRGPLIRDSITAGGATVAVRVPAHPVAAGLIRAVGTGVAAPSANRFGRISPT
                     RAADVVDELADRLAPGDVVVDGGPTPLGIESTVVDLTGPRPTVLRHGGVSVEDLVEVL
                     GALDAPERRTVDDDSASPAPGGLLRHYAPATPLVLVDGDRQRVEELAVALAERSVPAR
                     SLDLPADPAAAAAVLYRTLREADGTASVLLAAVVAPEGVGRGVNDRLFRAAHGRVVTD
                     SSPGTVDRLVALARA"
     gene            6390..7178
                     /locus_tag="PLZ72_08935"
     CDS             6390..7178
                     /locus_tag="PLZ72_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36458.1"
                     /translation="MLLVALVLAVTVAACAPTGTTEISSAPDGYPDQAPDGYPSQYPD
                     GYPSPQVMAEVTLPDGSVATVPQPSVPPQLDTSDAVPAPEPAAASAPAPAPRAPERPT
                     DDRAVQQLTDAAAIVASSRSLALLRTDWRAALPGWQIRFLPGRKGLRGATYPDRRVIE
                     IYVRSGDRPDELAHVVAHELGHAVDVARLDDADRAAWRAARGVPANVRWFPEGSAVAD
                     YATPAGDWAESFAHWQVGSGWFSRVGPPPDAAQQVLIARLAGLG"
     gene            7294..8226
                     /locus_tag="PLZ72_08940"
     CDS             7294..8226
                     /locus_tag="PLZ72_08940"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNA36459.1"
                     /translation="MTEQTTASHRGTSGDDPGRPSTIRGLIKACRPKQWAKNVLVFVA
                     PAAAGVLDDPYYLKHTLVGFVAFCAVSSATYLLNDTLDVESDRRHPTKCRRPIASGVV
                     PIPLALALAAVLAVLGCALAGFISWQLLAVVAGYAVLTTCYSAFLKQVPILDLVILSA
                     GFILRLLGGAYAAGVEVSEWFLLVSLFGSMFIAAAKRYAEKRELGDRAAELRPTLGEY
                     SFEFLALVRTSAIAAVTMSYCLWAVERGQMHGGAATPWIQLSIIPFVIAILRYALLVD
                     QGHGSAPEEVFAEDRMMQLMGVLLALFLFVGLYL"
     gene            8223..9596
                     /locus_tag="PLZ72_08945"
     CDS             8223..9596
                     /locus_tag="PLZ72_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA36460.1"
                     /translation="MSRVDPTAGREQLLWGWGRTSPARATVVAPERAEQVVDVVRSVG
                     PTGVVARGLGRSYGDPAQNAGGVVLDGTSRSGLLELDLARGIARMEAGTSLDDLMKWL
                     VPLGWFVPVTPGTRIVTVGGAIASDVHGKNHHKKGSWGNHVLSLRLVDGRGEVRDLSP
                     TEEPDLFWATVGGMGLTGIILDATVQLQAVSSSRILVDSDRAADLDEVMALMIDGDDD
                     YDYSVAWIDVMAKGRRLGRSVLDRGRFATRDEVLAAGVHRPADYAPHHLASVPDLFPS
                     GLVNHLTVKAFNELWFRKSPARRRDALMGIEHFFHPLDMVSDWNRVYGRRGFIQWQYV
                     VPDEASDFVRYSLERISGAGLPSFLSVLKRMGPGNAGHLSFPIAGWTLTIDIPVTAGL
                     DELFDDLDRRVADLGGRLYLAKDSRMDPALLAQMYPRLEEWREIRDKADPQHRFRSDL
                     GRRLGLV"
     gene            complement(9636..11138)
                     /locus_tag="PLZ72_08950"
     CDS             complement(9636..11138)
                     /locus_tag="PLZ72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNA36461.1"
                     /translation="MGHLRHRPRLRWLVGFVAAFALVATACGGGGSDDGDDQGSGGDS
                     AGVVDAADCPVKEVSSSTAPTNITVWHAYVGLQASTLQQIADAFNASQDKVKVDVQAQ
                     GTYEELLKKFQDALANPASLPDLVLTEDTTTQFMIDSGVVVPAASCREADPGSDATYD
                     DVLPAVTSAYTVDDVLWPGAFSVSMPVMYVNKAILAQADVPTDSYPQTIEELRSVAEK
                     IKAANVPGLKTPLVIKVDSWFVENWLTGAGKPLVDNDNGRSGLATESELVQPDTTRIY
                     EWFKSMIDDGLATAIPSSSTGVDEYLALNAKSSAILIQTSTAISTVAALLEGSVSKDQ
                     LGGVGIDATINTGLDIDVALQPGLEEAGQGQIGGSAWYMVKTDEKLGVPAAWQFIRYY
                     LQTPNQVTWTLQGSYLPVLKSAQDDPTLQKEFTTTTKGKWLNTAYQGLKNLNPDFPGP
                     VIGPFNEFRAIMRTSLDSVAFNGVGVKDAIKKADTDFQASLDQYKTDTGG"
     gene            complement(11156..12049)
                     /locus_tag="PLZ72_08955"
     CDS             complement(11156..12049)
                     /locus_tag="PLZ72_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNA36462.1"
                     /translation="MATDVTTLDGSSDAAPVAGPRPGRARSRWAAAGWYLVLTLLSVI
                     VLFPVYMLLLRAISDPLAYVAEGQPLHPVAVEWDAFTNAFTEASLGRSMALSFVVTLV
                     IVVAQLLTSVLAAYAFAFLEFPFKRVLFAVVIATLLLPIEVTLVANLQTVRDIGLLNS
                     VPGLSVPFLASAFGIFLIRQGFLGIPRDLRDAATLDGYGHLRFLFRVAVPLTRPIIGS
                     FVVISFLGAWNQYVWPRFATTEDSWQTVQVALRSIANDRVDQLNIGFAAAVIAALPLV
                     VLLILFQRQIVRGLTAGAVKG"
     gene            complement(12039..12980)
                     /locus_tag="PLZ72_08960"
     CDS             complement(12039..12980)
                     /locus_tag="PLZ72_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNA36463.1"
                     /translation="MTDLPVTEVLADDGEPIAVPLGSRTRSPARRVTDALLGYLFLLP
                     ALVVFGLFAFFPLGRLVFDSVHRQPRFLNRPPTYQGFGQLKDTLTSDQFTSGLTHSAQ
                     FMLYSVPLGLVLGVLLAVAAHRRLRGIKVFQAIFSSTVASSVAVASVIFFTLVNPQVG
                     YFKDVSWLSLDQPQSAMFSVALSSVWQNLGLTFIIVLAALQAVPEELNEAATLDGFGA
                     VRRFFRITVPLISPALLFLAIVLVISALQAYAQIEVLTGGGPAGATETLLFKIADPKG
                     IRPLHERAGLSLGLFALTGVVALVQYSLLSRRVHYGD"
     gene            complement(12977..14059)
                     /locus_tag="PLZ72_08965"
     CDS             complement(12977..14059)
                     /locus_tag="PLZ72_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36464.1"
                     /translation="MSGVTFAQVTKAFGSVTAVDSLSLEIVDGEFMVLLGPSGCGKST
                     ALRMIAGLEEITSGMLSIGDRVVNHVPPRERDIAMVFQSYALYPHMTVRKNIESPLVA
                     NRSARVDAAEREQRVQEAARALDLTDYLDRKPGELSGGQRQRVALARAIVRRPEVFLM
                     DEPLSNLDAKLRTQTRLELVELHRRLGTTIVYVTHDQVEAMTMADRIAIIDHGRLQQV
                     GAPQAVYDRPANLFVAGFVGSPPMNTLEGTVTFVGGEPVITTGHGALAASQAGTAAEG
                     DAVVVGVRSEHLRIGSGPAALTGEVENVELLGHERHVVVRVGDALLVVRQPTDAGAAV
                     VGETVTLVAEPTAVHLFDPATTERVN"
BASE COUNT         2095 a         4878 c         5108 g         2100 t
ORIGIN      
        1 ctcccggcct cgtcaccacc aggcggacat cacctggcat gggacctaag cgcgcgggga
       61 tccttggagc ggccgagatt cctcggctct ggtgcggttg agtggcagcc gctgctggat
      121 cgtgacgaca ggtgctgctc gatgccgatc agacgtggat cacgggccga gcttgcatag
      181 gtcgttgccg aggaactcga cgacgtcgct cgccgggaat agacgatggt gtcgccacat
      241 acgtcgagcc tgagcgttgc tcgacgaccg aggtgaccgg cccgtcgggt caggtgtaca
      301 tgctgcacat cgaggcgtgg agagaggacg aagtccgtcg cacggtgcac ctgtcggtca
      361 tcggcaaccg tggcagcggg ctccgcttgt caggcaaggg cttgtggcga ctcctctagc
      421 cgcctgccga cgacacgctg tcggaccaga gttgacgcgt cacgttcggc cgcttggccg
      481 agcagcgcta ctcgccgtcg atgagaccgc ccaggaggtc ggcggcgtct cggtctgcct
      541 ccgggacgaa gtggctgtag atcgacagcg tcgtggacgg gctggcgtgg ccgaggcggt
      601 tggcgacggt cctcgggtcc acgccgttcg cgatcagtcg ggtcgccacg taatggcgca
      661 ggtcgtgcag ccgcacgtcg tccagtccga gcttgtcccg gagcttggtg aagcggcggg
      721 tggcgctgtc gggtcgccag gggagcgacc catcgatctc gtagctcagc acgtacgcgt
      781 cgtcgaccaa ctccgtgccg ccctctcgcg cccggtcttc ccagcgctct cggtgctggt
      841 cgagcagcgc agcggtggca tcgtcgatcg ccactcggcg gacgcggtcg gtcttggtct
      901 ccttgcggac gacctggcca tcaccgatga ccaccgacga gttgatttcg accgagtgat
      961 gatcactggt gaggtcggcc caacggatgc cgaggacctc accacgacgt gcgccgagca
     1021 ccgctgccag ccgcacgaac acgtagaagt ccggatcctg ctcctcggcc gccttcaaca
     1081 ggcgggcgag gtcctcgggc gtcggcggct tgggctcttt gcgtcgaccc ttcggcggcg
     1141 aggcgttcgc ggcggggttc gacccgatcc actcccaccg gaccgcctgc tgcagcgctg
     1201 ctcgggcgac ggtgtggaac ttgcgaacgg tggcgggcga gagcggcttg cggtcgccgg
     1261 cgccggcgcg gagcgatccg tacagcgcgt cgaggtcgga ggcccggagg ttggccagct
     1321 tcttcttgcc gagcgccggg tcgaggaccg agtcgatgat gcgacggtgt tcaaccgccg
     1381 tgcgcggaga ccagtccggt gacacggcgt cgtaccacgc gtcgagcagc tcgccgaacg
     1441 tggccgagct cggcgacgcg gtgccgttct gaacctggac gagcagctgg ttgcgaaccc
     1501 gctcggcgtc cttgcggctg ccccgcacgg tgcgggtgat ctggcgcttg ctgccggtca
     1561 ccgggtcacg accggcgtac accatcacgc ggaatgaacc attgcgctcg acgatgtggc
     1621 cgctggacat gccatcacgg taccggccgg ccctgacggg attaccaaat gggttaccaa
     1681 tcggcccatc gggatccggc ggcctcagcc cggaaacgac gaaagccctg caattgcagg
     1741 gctctcggtc tggcaccccc aacgggattc gaacccgtgc cgccaccttg agagggtggt
     1801 gtcctaggcc gctagacgat gggggcctac gtccgaccag gaggtcggcg atatgtggat
     1861 ccgaccggag ccggagatct gtcgatctgg ttcggggggg aggactcgaa cccccaataa
     1921 ctggaccaga accagttgtg ttgccgatta caccaccccc gaaaggtggt gcgccccgaa
     1981 gagcgccgga gcactctagc cgaggcccac cgggccgacg gaaatcggat ccgaccctcc
     2041 cgccgccccc agtgaccgat ggccccgacc agggtcgggt cccgcggtct accgtgagcg
     2101 gtgcgggatc gaagggggcg acgatgaccg gcgcgacgtc atccgacgag gacatggatc
     2161 tggacctgga ctggtcgggc ctcgaggtcc tcacctacga cgaatgccgc tacctcttgt
     2221 cgatcgagcc ggtcggccgg ctcggtttcg tgcacgacgg ctccccggtg gtgctgccga
     2281 tcaactacgt gctcgacggc ggatcgatcg tgttccgcac cggacggggc tccaagttgg
     2341 agacggccac gatgggtcgg gcggtgtgcg tcgagatcga ctcgtggaac gcgctggagc
     2401 acacgggctg gagcgtcgtg gccaagggcc acgccgagca cgtggccgac ggggccgagc
     2461 tcgaacggct ggcccggctg ccggtcaagc cctggtgtcg ccccgacgtg cgccgcaact
     2521 gggtgcgggt gatgatcgag gagctgagcg gccgccgcat ccaccagtga cctgaccggc
     2581 ccgaccgggc gacccccatc agaacccggc tcgctccatg aacgcgtcgc cggtgttgta
     2641 ggccgtctcg gcgtgctcgg cgacgtccag gccggtcgcc tcgacctcct cggtgacccg
     2701 caggccgatg gtcgccttga tcgccagcag gatcaccgcg gtgacgatgc cggagtagac
     2761 gatggtgacg ccgttggcca gcgcctgctc ggccagcagc tcgagcccgc cgccgtagaa
     2821 gagcccgttc atgtgctcgg cgccgaagta gtcggcatcc gcgaagagcc cgatcatgag
     2881 cgagccgacc aggccgccga cgaagtgcac gccgaccacg tcgagggcgt cgtcgaagcc
     2941 ggccctgaac ttgatgtgca cggcgtagca gcagatcacg ccggcggcga ggccgatgaa
     3001 gatcgacgac atcgacgaca cgtagccgca cgccggggtg atggcgacca gtccggcgac
     3061 gatgcccgac gccgctccca ggttggtgaa gtgcccgtcc ttgatgcgct cgacgatcgc
     3121 ccacgccaga ccggcggcag cggcggccag gaacgtgttc atgaaggcct ggaccgcgac
     3181 gccgttggcc gacagggcgg agccggcgtt gaagccgaac cagccgaacc acaggatgcc
     3241 ggtgccgatc atcaccaacg gcatggagtg cgggggatgg ccctcgcccg gccaaccctt
     3301 ccgcttgccc aggatgagca cggcgaccag cgccgcgatt ccggcgttga cgtggatggc
     3361 ggtcccgccc gcgaagtcga gtgaaccccg cacgccgagc cagccgccgt agacccactt
     3421 gaagaccggg gcgaacacca gcagcatcca cacgggggcg aacaccgccc atgccgcgaa
     3481 cttcatgcgg tccgccacgg cgcctgagat cagcgccggg gtgatcacgg cgaacatccc
     3541 gaggaacgcg accagcatga gcgtgtcggc gccggactgc gaggacaggt tcaggttccc
     3601 caggaacgcg gcgtcgaagc cgccgaacag ggacccggcg gtgtcgccgt acgcgatcga
     3661 gtagccgatc accacccaga cgatggggat gatgagcagg caccagaagt tcatcatcaa
     3721 catgttcagc acgttgcggc tccggtccat gcctccatag aagaaggcga gtcccggcgt
     3781 catgaacagc acgagtgccg ccgagatcag caaccagccg tcggctccga gttctgtggc
     3841 catggccctt tccgtttcgg gatcgtggcg tgggggcgta ccgcccgcac ggtcgccgtc
     3901 ggcgacaccg gcactttcgt gtcaccgcgt ttcgtgcgtg tttcgccggt ggggcgacgg
     3961 tgtgaaggtt gccggggagc tcgccgggtc gcgtggcgtc cgccggcgcg aggctggtgg
     4021 catgtgccgg aacatcacga ccctgcgggg cctcgaaccc gcggcgacgc ccgaggagat
     4081 cgaggcggcc gcccgtcagt acgtgcgcaa ggtgagcggg gtgcagtcga cctcggcggc
     4141 caccgaggtg gcgttcgaac gcgcggtggc gctcgtcacc gacgcgacgg ccgagctcct
     4201 ggcccagctg cccccgcgca aggtgccgcc gaagaacgac ccgccgttgc ggcgactggc
     4261 cgcacgcggg gcctgagcgg ggtcagtcct tcaggccggg gtgccgactc gcgagcagcg
     4321 gcacgagcag ctgcttgggc accagcccga ccagcgacgc gctcacccgg ttggccgggc
     4381 cggggatggc gaccgggcgt cccgccgcga ggccgtcgac accgcagcgc gccacggcat
     4441 cggcggactc ccacatgaac gacggcagcg acgactcggc gtcctcggcc gagatccccg
     4501 ccgcgtcggc gaacccggtg tgcaccggac ccgggcacag cgcggtggcc gtcaccccgg
     4561 tgtcgcgcag ctcgccgccg agcgagcggg tgtaggagag cacgaacgcc ttgcccgcgg
     4621 cgtatccggc ctggcccggt agcggttgga acgctgcggt cgaggccacg ttcagcaccg
     4681 ccccggtccg gcgctccacc atcccgggca ggaaccggct gcacaggtcg accaccgcca
     4741 cgacgtcgac ctcgaccatg ttgagctcgg cggccggatc gctgcggtgc accggcccga
     4801 ccgtcgacag cccggcgttg tttaccagga tgctgaccgt ccggccgttg gcggcgacgc
     4861 ggtcgggcag ctccgcccgg gcgtcgcggt ccgacaggtc ggccgccagc acctcggcgg
     4921 tcacccccag cgccgacagc tcggtggcca gctcatgcag cttgtcggcg cggcgggcga
     4981 ccagcgtgac gttctggccc cggcgggcca gctcccgggc gatctcggtg ccgatcccgg
     5041 aggaggctcc ggtgaccagg gcggtgcggt cgggggcggg ggacggcagc ggcatggccg
     5101 cgacgctacc ggtggggggg tcggtggtgc cgtgccgacc acgggccacc acgatcgggt
     5161 gggtagcgtg cggccccgat ggacccacgg cctggccacc ccgaggtgtt ggcgctggat
     5221 ccggtccggc cggaccccga acagctcgcc gcgctggccg accgcatggc cgccgggtcg
     5281 ctggtcgcgt tcccgaccga gacggtctac gggctcggtg cccgcatcga ccgtcccgcc
     5341 gcgctcgacc ggatcttcgc ggtcaaggga cgtccggcga ccgacccgct gatcgtgcac
     5401 gtcgcctcga tcgacgcgct cgacgggatc gtcgggcggg tgccggatcg gttgacggcg
     5461 ctggcccggg cgttctggcc cggtccgctc accgtggtgc tcgatcgcgg tcccctcatc
     5521 cgcgacagca tcaccgcggg tggcgcgacc gtggcggtcc gggtcccggc gcatcccgtg
     5581 gcggcgggtc tgatccgggc cgtcggcacc ggggtggcgg ctccgtcggc gaaccggttc
     5641 ggacgcatca gtcctacccg ggcggcggac gtggtcgacg agctggccga ccggctggcg
     5701 cccggggacg tggtcgtcga cggtggaccc accccgctcg gcatcgagtc gaccgtcgtc
     5761 gacctgaccg gcccgcgtcc gacggtcctg cgccacggcg gggtgagcgt cgaggacctc
     5821 gtcgaagttc tcggcgcgct cgacgcgccg gagcggcgca ccgtcgacga cgactcggcg
     5881 tcccccgcac cgggcggtct gctgcgccac tacgcgcccg cgacgccgct ggtgctggtc
     5941 gacggcgacc gacagcgggt cgaggagctc gcggttgcgc tcgccgaacg atcggttccg
     6001 gcccgatcgc tcgacctgcc ggcggatccg gccgcggccg ccgcggtcct gtaccgcacc
     6061 cttcgtgagg ccgacgggac cgcatcggtg ctgctcgctg cggtcgtcgc tcccgaaggg
     6121 gtcgggaggg gagtgaacga ccggctcttc cgggccgccc acggcagggt ggtcaccgat
     6181 tcctcccccg ggaccgtgga ccggttggtg gctctcgcgc gagcatgacc gccgacaggt
     6241 cggggcgatg cgggggcgcg ccggcggctg agcgtgcccc gggggaaccg gacatgggcg
     6301 aaccacaacc cacccgtcga tcgacgccgc gccgttccac gcgggcggga gcgccgtcgc
     6361 cggccaggac gcgtcgatcg gctccgtcgg tgctcctcgt cgcgctggtg ctggccgtca
     6421 cggtcgcggc ctgcgccccg accggcacga ccgagatctc ctccgctccc gacggctacc
     6481 ccgatcaggc tccggacggc tatccgagcc agtacccgga cggctacccg tcgcctcagg
     6541 tgatggccga ggtgactctc cccgacgggt cggtcgcgac cgttccccag ccgtcggtgc
     6601 ctccacagct cgacacgtcc gacgccgtcc cggctcccga gccagccgcc gcatcggcgc
     6661 ccgctccggc gccgcgtgcc ccggaacgcc cgaccgacga ccgtgcggtg cagcagctca
     6721 ccgacgcggc ggccatcgtc gcgtcgagcc gttcgctcgc cctgctgcgc accgactggc
     6781 gcgccgcgct gccggggtgg cagatccggt tcctgcccgg ccgcaagggc ctgcgcggcg
     6841 ccacctaccc cgaccgccgc gtcatcgaga tctacgtccg ctccggtgac cgtcccgacg
     6901 agctggccca cgtcgtcgcc cacgaactgg gtcacgccgt cgacgtcgcc cgcctcgacg
     6961 acgcggatcg ggcggcctgg cgcgccgccc gcggcgttcc ggcgaacgtc cggtggttcc
     7021 ccgagggctc ggcggtggcc gactacgcca cgccggcggg ggactgggcc gagagcttcg
     7081 cccactggca ggtcggctcc ggctggttct cccgggtggg accaccaccc gacgcggcgc
     7141 agcaggtcct gatcgcccgc ctggccgggc tcggctgagc tgtcgggccg gccggtcccc
     7201 gaaggtcggg ggtctcgggg atggggaacc cggccgatac actgacccgg tcctggacct
     7261 caggacctcg ggatcggacc acacggggct tcggtgacgg aacagacgac ggcatcccac
     7321 cgcgggacca gcggcgatga tcccggtcga ccgtcgacga tccggggcct gatcaaggcg
     7381 tgccgcccca agcaatgggc caagaacgtc ctggtgttcg tcgcgccggc cgctgcgggg
     7441 gtgctcgacg acccctacta cctcaagcac acgctggtcg gcttcgtcgc cttctgcgcg
     7501 gtgtcctccg cgacctacct gctcaacgac acgctcgacg tcgagtcgga ccgccggcac
     7561 cccaccaagt gccgccgccc gatcgcgtca ggcgtggtgc ccatcccgct cgctctcgct
     7621 ctggccgcgg tgctggccgt gctcgggtgc gctctggccg gcttcatcag ctggcagctg
     7681 ctcgcggtgg tggcgggcta cgccgtgttg accacctgct actcggcgtt cctcaagcag
     7741 gtgccgatcc tggacctggt catcctgtcg gcgggcttca tcctgcggct cctcggcggc
     7801 gcctacgcgg cgggggtcga ggtctcggag tggttcctcc tggtgtcgct gttcgggtcg
     7861 atgttcatcg ccgccgccaa gcgctacgcc gagaagcgcg agctcggcga ccgtgccgcg
     7921 gagctgcgcc ccacgctcgg cgagtacagc ttcgagttcc tggcgctcgt gcgcacctcg
     7981 gcgatcgccg cggtcaccat gtcgtactgc ctgtgggcgg tcgagcgcgg ccagatgcac
     8041 ggcggggctg ccacgccgtg gatccagctg tcgatcatcc cgttcgtgat cgccatcctg
     8101 cgctacgcac tgctggtcga ccagggtcac ggatccgccc ccgaagaggt cttcgccgag
     8161 gaccggatga tgcagctcat gggggtgctg ctggccctgt tcctgttcgt ggggctgtac
     8221 ctgtgagccg ggtcgacccg accgccggac gggagcagct gctgtggggc tggggtcgga
     8281 cctccccggc ccgtgccacc gtcgtcgcgc ccgagcgcgc cgaacaggtc gtcgacgtgg
     8341 tccgctcggt gggtccgacc ggggtcgtcg cccgcggtct gggccgttcc tacggcgacc
     8401 cggcccagaa cgccgggggc gtggtcctcg acgggacctc ccgctccggt ctgctcgagt
     8461 tggacctcgc ccgggggatc gcccgcatgg aggccggcac cagcctggac gacctgatga
     8521 agtggctcgt gccgctcgga tggttcgtgc cggtcacccc gggcacccgg atcgtgaccg
     8581 tcggcggagc gatcgccagc gacgtgcacg gcaagaacca ccacaagaag ggctcgtggg
     8641 gaaaccacgt gctgagcctc cgcctggtcg acggccgggg cgaagtccgc gacctcagcc
     8701 cgaccgagga gccggacctc ttctgggcga ccgtcggcgg catgggcctg accggcatca
     8761 tcctcgacgc caccgtgcag ctccaggcgg tcagctccag ccgaatcctg gtcgacagcg
     8821 accgcgccgc ggacctcgac gaggtcatgg cgctgatgat cgacggcgac gacgactacg
     8881 actactccgt ggcgtggatc gacgtgatgg ccaagggccg ccggctgggc cgctcggtgc
     8941 tcgaccgggg ccgattcgcc acccgcgacg aggtgctcgc cgccggggtg caccggcccg
     9001 ccgactacgc cccgcaccac ctcgcgtcgg tgcccgacct gttcccctcg ggactggtca
     9061 accacctgac ggtcaaggcg ttcaacgagc tgtggttccg caagtcaccc gcccggcgcc
     9121 gcgatgccct gatgggcatc gagcacttct tccacccgct cgacatggtc tcggactgga
     9181 accgggtcta cggccggcgt ggcttcatcc agtggcagta cgtggtgccc gacgaggcga
     9241 gcgacttcgt ccggtactcg ctcgagcgga tctcgggtgc gggcctgccg tcgttcctgt
     9301 cggtgctcaa gcggatgggc ccgggcaacg ccgggcacct cagcttcccg atcgccggct
     9361 ggaccctcac catcgacatc cccgtcaccg ccggcctcga cgagctcttc gacgacctcg
     9421 accgtcgggt ggcggatctc ggtggacgcc tctacctggc caaggactcc cggatggacc
     9481 cggcgctcct ggcgcagatg tacccgcggc tcgaggagtg gcgcgagatc cgcgacaagg
     9541 cagatcccca gcaccggttc cgcagcgacc tcggccgtcg gctgggcctc gtctgacccg
     9601 ggctctcgac ggagtccgcc ggcggaggcg ggtgctcagc ccccggtgtc ggtcttgtac
     9661 tggtcgaggg acgcctggaa gtcggtgtcg gccttcttga tcgcgtcctt cacgccgacg
     9721 ccgttgaacg ccacgctgtc gagcgacgtg cgcatgatgg cccggaactc gttgaacggg
     9781 ccgatgaccg gaccggggaa gtcggggttg aggttcttta ggccctggta ggcggtgttc
     9841 agccacttgc ccttggtggt ggtggtgaac tccttctgca gggtcgggtc gtcctgggcg
     9901 gacttcaaca ccggcaggta ggagccctgc agggtccagg tcacctggtt cggggtctgc
     9961 aggtagtacc tgatgaactg ccacgcggcg gggacgccca gcttctcgtc ggtcttgacc
    10021 atgtaccagg ccgatccgcc gatctggccc tgaccggcct cctcgaggcc gggctgcagg
    10081 gccacgtcga tgtccaggcc ggtgttgatg gtggcgtcga tgccgacgcc acccagttgg
    10141 tccttggaca ccgacccctc gagcagggcg gcgaccgtcg agatggcggt ggaggtctgg
    10201 atcaggatcg cggagctctt ggcgttgaga gccaggtact cgtcgacgcc ggtcgacgag
    10261 ctcgggatgg ccgtggcgag gccgtcgtcg atcatcgact tgaaccactc gtagatgcgg
    10321 gtggtgtcgg gctggaccag ctcggactcg gtcgccaacc cgctgcggcc gttgtcgttg
    10381 tcgaccaggg gcttgccggc gccggtcagc cagttctcga cgaaccacga gtcgaccttg
    10441 atcaccagcg gggtcttgag acccggcacg ttcgcggcct tgatcttctc ggcgaccgaa
    10501 cgcagctcct cgatggtctg ggggtagctg tcggtgggca cgtcggcctg ggccaggatc
    10561 gccttgttga cgtacatgac cggcatcgag acgctgaagg cgccgggcca cagcacgtcg
    10621 tcgacggtgt aggccgaggt gaccgcgggc agcacgtcgt cgtaggtggc gtcggacccg
    10681 gggtcggcct cccggcacga cgcggcgggg accacgactc cggagtcgat catgaactgc
    10741 gtggtggtgt cctcggtcag caccaggtcc ggcagcgacg ccgggttggc cagggcgtcc
    10801 tggaacttct tgagcagctc ctcgtaggtg ccctgggcct ggacgtcgac cttcaccttg
    10861 tcctggctgg cgttgaacgc gtcggcgatc tgctgcaggg tcgaggcctg aaggcccacg
    10921 taggcgtgcc agacggtgat gttggtgggt gccgtcgacg acgacacctc cttcaccggg
    10981 cagtcggccg cgtcgacgac accggcggag tcaccgcccg agccctggtc gtcgccgtcg
    11041 tcggaaccac ccccaccgca ggcggtggcg acgagggcga aggccgcgac gaacccgacc
    11101 agccagcgga ggcgtggtcg atggcggagg tgacccatgg tgctctctct ttctgtcagc
    11161 ccttgaccgc gccggcggtc aggcctcgga cgatctgacg ttggaacagg atcagcagca
    11221 ccaccagggg cagcgcggcg atcaccgcgg cggcgaagcc gatgttgagc tggtcgacgc
    11281 ggtcgttggc gatcgagcgc aacgccacct gcacggtctg ccagctgtcc tcggtggtgg
    11341 cgaagcgggg ccacacgtac tggttccacg cgcccaggaa cgagatcacc acgaagctgc
    11401 cgatgatcgg gcgggtgagc ggcaccgcga cgcggaacag gaagcgcagg tggccgtagc
    11461 cgtcgagggt ggcggcgtcg cggaggtccc gcgggatgcc caggaagccc tgtcggatca
    11521 ggaagatgcc gaacgcgctg gccaggaacg gcaccgacag cccgggcacc gagttgagca
    11581 gaccgatgtc gcggacggtc tggaggttgg cgacgagggt gacctcgatc ggcagcagga
    11641 gggtggcgat gaccacggcg aacaggaccc gcttgaacgg gaactcgagg aacgcgaacg
    11701 cgtacgcggc gagcaccgac gtcagcagct gggccacgac gatgaccagg gtcaccacga
    11761 acgacagggc catcgagcgg cccaggctgg cctcggtgaa cgcgttggtg aacgcgtccc
    11821 actcgacggc cacggggtgc agcggctggc cctcggcgac gtaggccaac gggtccgaga
    11881 tggcccgcag caacagcatg tagacgggga acaggacgat gaccgacagc agcgtcagca
    11941 ccaggtacca accggcggcc gcccagcggg agcgggcccg accgggtcgt ggccccgcta
    12001 ccggtgccgc atcggaggat ccgtcgagag tcgtcacgtc agtcgccata gtgcacccgt
    12061 cgtgacagca gggagtactg caccagggcc accaccccgg ttagggcgaa cagccccagg
    12121 gacagcccgg cgcgttcgtg cagcgggcgg atgcccttgg ggtccgcgat cttgaacagc
    12181 agcgtctcgg tggcgcccgc gggcccgccg ccggtcagca cctcgatctg cgcgtaggcc
    12241 tgcagggcgc tgatgaccag gacgatggcc aggaacagca acgcggggga gatgagcggc
    12301 acggtgattc ggaagaaccg tcggaccgcc ccgaacccgt cgagggtggc ggcctcgttc
    12361 agctcctcgg ggacggcctg gagcgcggcg agcacgatga tgaaggtcag ccccaggttc
    12421 tgccagaccg agctgagggc gaccgagaac atggcggact ggggctggtc gaggctcagc
    12481 cagctgacgt ccttgaagta gccgacctgc gggttgacca gggtgaagaa gatgacactc
    12541 gccaccgcca ccgacgaggc gacggtggag gagaagatcg cctggaacac cttgatgccc
    12601 cgcaaccgcc ggtgcgcggc gaccgccagc agcacgccga gcaccaggcc cagcggcacg
    12661 ctgtagagca tgaactgcgc cgagtgggtg aggcccgagg tgaactggtc gctggtcagc
    12721 gtgtccttga gctgcccgaa gccctgatag gtggggggtc ggttgaggaa ccggggctgt
    12781 cggtgtaccg agtcgaacac cagacggccg agcgggaaga acgcgaacag gccgaacacg
    12841 accagtgccg gcagcaggaa caggtagccg aggagcgcgt cggtgacccg gcgggccgga
    12901 gagcgggtgc gcgacccgag cgggacggcg atcggttcgc cgtcgtccgc gagcacctcg
    12961 gtcaccggca ggtcggtcaa ttgacccgct ccgtggtcgc ggggtcgaac aggtgcacag
    13021 cggtcggctc ggcgacgagc gtcaccgtct cgcccacgac cgcggcgccg gcgtcggtgg
    13081 gctggcgcac caccagcagg gcgtcgccga ctcggaccac gacgtgacgc tcgtggccca
    13141 gcagctcgac gttctccacc tcgccggtga gcgcggccgg gcccgatccg atgcggaggt
    13201 gctcggagcg cacgcccacg acgacggcgt cgccctcggc ggcggtgccc gcttgcgagg
    13261 cggccaacgc gccgtggccg gtggtgatca cgggctcgcc gccgacgaag gtgacggtgc
    13321 cctcgagggt gttcatgggc gggctgccga cgaagccggc gacgaacagg ttggccggcc
    13381 ggtcgtagac cgcctggggt gcgccgacct gctggagccg cccgtggtcg atgatggcga
    13441 tgcggtccgc catggtcatg gcctcgacct ggtcgtgggt gacgtagacg atcgtggtcc
    13501 ccagccgtcg gtgcagctct accagttcga ggcgggtctg ggtgcgcagc ttggcgtcga
    13561 ggttggacaa cggctcgtcc atcaggaaca cctcgggtcg ccgcacgatg gcgcgggcga
    13621 gcgcgacccg ctggcgctgg ccgcccgaga gctccccggg cttgcggtcc aggtagtcgg
    13681 tgaggtccag cgcccgcgcc gcctcctgga cccgctgttc gcgctcggcc gcgtcgaccc
    13741 gggccgacct gttggccacg agcggcgact cgatgttctt ccgcaccgtc atgtgcgggt
    13801 agagcgcgta gctctggaac accatggcga tgtcgcgctc gcgcgggggg acgtggttga
    13861 ccacccggtc accgatgctc agcatccccg aggtgatctc ctcgaggccg gcgatcatgc
    13921 gcagcgcggt cgacttgccg cacccggagg ggccgagcag caccatgaac tcgccgtcga
    13981 cgatctccaa cgacaacgag tcgaccgcgg tgaccgaccc gaaggccttg gtcacctggg
    14041 cgaacgtgac accgctcatc gtggaaccaa cctgtgatcg tctcgtcggg cggcagctcg
    14101 atctgccgca gtgggtccgc ggcagactag gcggcgcacg cctcgccgcg gctgaccggg
    14161 gtgtcgggca tgggtcaccc g
//
LOCUS       DAOQOD010000077        14079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1541_length_14079_cov_12.337208, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000077 DAOQOD010000000
VERSION     DAOQOD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_14079_cov_12.337208"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1703
                     /locus_tag="PLZ72_08970"
     CDS             162..1703
                     /locus_tag="PLZ72_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNA36465.1"
                     /translation="MPVADGRGGFNGAVLFPTMTFAIFFAIVLGVSWRIADRPLAWRW
                     FVLAASYVFYGWWDARFCLLIAGSTLINHLVVNLVARARTDRAARVSMIVGVGANLVV
                     LGFFKYYGFFVDSLLQILEPVGLAPTALLIKVGLPVGISFFTFCAISYVVDVYRQEQE
                     PIALLDFAVYLSFFPHLVAGPIVRVSEFEPQLRRRRNRERVDAVRAARLICRGMFKKV
                     VIANYLATAIVDPVFASPGQSPNLELAVAAVAYAVQIYADFSGYTDMAIGIALLMGIR
                     FPDNFDQPYSATSIQDFWRRWHMTLSRWLRDYVYIPLGGNRGGERAEMRNLVATMAIG
                     GLWHGANWTFVVWGFYHGFGLVAERKWRERTPRSWRRGAIDLRNPDADDDTAAADGYT
                     RVMARPTVRPEPNVWVSRAAVFAFVTVGWVFFRAVDLPTAIGYLVGLVTRWGVGDLVT
                     PLVLVTIVVGMVGQFVPRRVGDALEYRASHLPPVLLGIGVGLFLVVCNLLGPVGVAPF
                     IYFQF"
     gene            1753..2817
                     /locus_tag="PLZ72_08975"
     CDS             1753..2817
                     /locus_tag="PLZ72_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HNA36466.1"
                     /translation="MRVTDDDRGADRAARSGPDAPKGRPASRPGRPRRRVRRTMSATT
                     AWKSYLVALVVFAVLGSGGLRESAEGMPLGWPRDVAMALTGGLDRAVNLVSLNRPYDW
                     AAERLGRTQADEDFEFPVPTTTPDATATTTTLPPLRTPTAADPLKVVVAGDSTAIGVG
                     DRLKVAVDDDPTLAVDVQGKVATGLTRSDYFNWGARTKELMETLSPDVVVFMVGANDT
                     QAVMAPDGTVVARYGTPGWADAYRRQVAGIMDLAHDGSRRLLWIGQPRVGNAKINATL
                     SQVNDIIQEEAAGRPWVTYFDLASVVAGPGGSFSEYVTFPDGQTVHCFAGDGVHLSMK
                     CLDRSMSELVPALRELYRTT"
     gene            complement(2840..3658)
                     /locus_tag="PLZ72_08980"
     CDS             complement(2840..3658)
                     /locus_tag="PLZ72_08980"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNA36467.1"
                     /translation="MNAALLVLSAVIGIVAGWQLTVPVHRYKELRPLTPRDVAAEVGT
                     ADQPPPVIRAEYRQARCPHCHHVYRAADVVPVLSWFRGCPSCGERLAFTVPLLQVVVP
                     VAMVLTVATTSSGWVALPYLWLVVVLAAVAIIDLRIWLIPWWMPWVGAAVGFVLICAM
                     SLVIGAPDAIVRAVISGVVMFGLFFVLWLVAPGKLGFGDVRLMFLLGLFLGWFSLVLP
                     IYGLLFGSLIGLAMGIGSLVARRGTRFPFGPALALGALMALWLNGPLLNSLGMG"
     gene            complement(3783..4280)
                     /locus_tag="PLZ72_08985"
     CDS             complement(3783..4280)
                     /locus_tag="PLZ72_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HNA36468.1"
                     /translation="MTDDVFPDRNMEHLDKLAWVIEDQGWVADPVEADAGPPPRPGYT
                     YTIGFETAFDRPEVVIFGLQAVAARGLLGMIADQHRGGVVLPVDGVFVGLLESDLPCA
                     LLTVDLAVHAARFPDASAFHAGRGVGADRWRMLQFVWPDRNGRLPWDQDYDPRLRVAQ
                     PVIGT"
     gene            complement(4277..5803)
                     /locus_tag="PLZ72_08990"
     CDS             complement(4277..5803)
                     /locus_tag="PLZ72_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA36469.1"
                     /translation="MLGEIVRTAADRFGDREVLRRATDALTYRDLDAAADRVAAGLVH
                     RGIGPGDVVSDVLPSCAEWVVLAVALDRIGAVHAPVSPKLTAPERAELVALVAPDLVV
                     TDRAGVEGLPLRSDVVVVEPGDRGEPLWGPRRPTTPDTGDRPGPTTDDPGRVTTICFT
                     SGTTGRAKGAVFTVAHQRAVQDLDLGEGAEQRWGGGAAMLASTQFAHVGMALKLPWYL
                     RTGSTLHVLDTWRADDALRLVAEHRMPTIGVVAPQLALMLRSPLMESLDLGSVSAIIA
                     GGAASPPGLVQEARERFGAAYSIRYSSTESGGVGLGTAFDADDDEALHTIGRPRAGVE
                     ARVTDEDDGPLPDGEVGELQIRSAAVMDRYWNDPDATAAALTTDRWLRTGDLAVRRDD
                     GCYVLSGRRTDMYIRGGYNVFPEEVEAALSDHPAVAAVAVAPRPDEVMGEIGVAVVVA
                     RDPATAPSLQDLRDHAATRLARHKLPEDIVVVPRLPLTTVAKLDRAALAGIVADRSPT
                     "
     gene            6003..7139
                     /locus_tag="PLZ72_08995"
     CDS             6003..7139
                     /locus_tag="PLZ72_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36470.1"
                     /translation="MSRDRHLRRSRSGSPTGRRALGALTLIGVAGLLAACAPASRNSS
                     GHGSTPALDASRAPAPAAPVTTRPTTYVTAGARAGIGGCDLFPVDHAFHASIASLPVR
                     SSSGATIAAIGPSETLLPGFSAATWMGSRSGYPTNLIDSRTAPRSFVAVASMYQSVSE
                     NQSVPWPDDPRFEGWPDRAWDRHLLTVDTHRCESWELINAQAPGENVFAALLHPGSWY
                     ADRVVRLDLSSNTIPPTGTVTASGLSMLAGLVRYDEVASSRVDHALSMVLPDIGAGAT
                     WPASRSDGRSTDPAAPPMGTWFRLRADVDISGLRPQARVVAQAMKDHGVVLVDTGPHA
                     AISGEPDVRWDDADLDGLRSLTIGQFEVVDPTPMKVADGSYRIR"
     gene            7202..8473
                     /locus_tag="PLZ72_09000"
     CDS             7202..8473
                     /locus_tag="PLZ72_09000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA36471.1"
                     /translation="MPAEADPVRPSPSGAAPLAGVRVIEVSLLGPGAVGTHLADLGAE
                     VIKVEPPAGDYIRQMTWPIIEGSSLLHWHIHRGKKSLTLDLRTDEAQDVFRDLVRDAD
                     AVIEAMRPGALARRGLGYEDLLEINPRLVFCTISGYGMTGPYANLPSHGIAYDTWAGI
                     VNPTFNDDGFPYLPEHASIGIHAGPLFGALGVLAGITKARATGEGSFLEIAQSDAAAA
                     MDWLRSETWKAYERPESEVTGNKADDYERRAPGTAGMEEGVRYQVYESSDGFVLFMAS
                     EQEFWKNFCTGVDRMDMFERWPGNTYGDHARGNTEMRRELAAIFATRTTAEWVEWSGT
                     VNTTIAPVNTPQTLADDPQFLDRFGFMPHETHGADMIGTPLKFLDAELPDPTPAPTAG
                     QHNDEVLGSVLGYDGERIAALRAAGALGEGN"
     gene            8525..10732
                     /locus_tag="PLZ72_09005"
     CDS             8525..10732
                     /locus_tag="PLZ72_09005"
                     /inference="COORDINATES: protein motif:HMM:NF005801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HNA36472.1"
                     /translation="MTAPIVVERTSRLAAPPGPVWAHASSMAGVNLELAPFVRMTHPT
                     DRDRLTGPQVSFTSWLLAFGLVPSDRHSFRLLEVADSGREGGSFREDSRSWMQARWRH
                     DRDVVAEGSGSRVTDRLEVTPRLAVARPLVAALVGFLFTHRHRRLRDRFGEERPGPDR
                     AARAPGTVAGDALGPAQQDDVVTEDGPMSTDATTPGEAAGDGTRGDLRWASVPALVRD
                     AAERHGTAEAVVDLAGDEPVRLDWAELRRRVDNMAKGLIAAGVEPGDRVAIWAPNCWE
                     WVVAVLGLQSAGAALVPLNTRYKGLEAADILARSRARLLLTVEGFLGNDYVSMLDAAG
                     RPDTLEGIVLLRDTGARPAGAITLDELLAGGDAVPDAELDARIAALDGNDVSDVLFTS
                     GTTGAPKGVVQTHAATLRAFLDWSTLVGLRAEDRYLIINPFFHTFGYKAGILACLMTG
                     ATMVPLPVFDVEAAIDAIEAERISMIPGPPTLYQTLLNHPRFDRSRVDTLRLAVTGAA
                     VVPVELVVAMEEQLGFETVLTAYGLTEACGVVTVCRREDGAETIATTSGRAIPGVEVV
                     IADPDGNRLGPDQPGEVLVRGYNVMREYFEDPEQTAEAVDPDGWLHTGDVGTLDARGY
                     LRITDRTKDMFIVGGFNAYPAEIEQLLGAHPDIAQVAVVGVPDERMGEVGVAFVVPTA
                     GAELDGDAVIAWAREHMANYKVPRRVEIIDALPLNASGKVLKFELRDRATSGS"
     gene            10746..11609
                     /locus_tag="PLZ72_09010"
     CDS             10746..11609
                     /locus_tag="PLZ72_09010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA36473.1"
                     /translation="MKFSQALLGANPLDWPRIAAAAEEAGFDSVAVSDHVVYPARLDS
                     RYPYTPDGVPLFSPDEDWPDPWVAVGAMSAVTTRLRFLTNVYVLPLRNPFVVAKAVGT
                     AAYLSRGRVGLGIGAGWMAEEFELMGQPFARRGKRMDEMVEVVRTLWRGGMVEHHGEF
                     YDFDPVEMRPAPPAPVPIYVGGHSEIAMRRAATLGDGWLGMYYSVEKLEQHCRTLERA
                     REDAGTADRPFEIIASPMVTPTAENCERLEAAGVTTILTSAWMAQGVLGPDSTEQAIE
                     LIATFGERFIA"
     gene            11727..12428
                     /locus_tag="PLZ72_09015"
     CDS             11727..12428
                     /locus_tag="PLZ72_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNA36474.1"
                     /translation="MSDDVRHLGTIEGREIHGASGPAAPPEPPPPRAGGAPVVHPGGP
                     PARPGRTVAVRAEVAEALHDIRDAHRRGPKPNTRPLPRWVERLAWVLDDAIEIPATGG
                     RRVGVDGFITLVPGIGDAVGVALSMVVVSAGVLAGVSWPTIIRMLWNVAFEGVVGLIP
                     FAGVVFDMAYKANDRNVRLIEADLADRAATRRSSLGVILALVLTSIVGFVMMVVLSLL
                     GITVMVWILWKLLGG"
     gene            12511..13239
                     /locus_tag="PLZ72_09020"
     CDS             12511..13239
                     /locus_tag="PLZ72_09020"
                     /inference="COORDINATES: protein motif:HMM:NF039921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA36475.1"
                     /translation="MATKSATEQVAAEGSATDPTDPTDPTESDELRAVDGRVPGRRGL
                     ATRARLLEQTRELLYTTSYRDLKVVDIARGAGTSPATFYQYFPDAESALWALADELVG
                     EGRERLTRPVRDGSWNGRAAYDTCERIAASFLEFWDEHRALLAVIDLAATEGDVRFRE
                     IRLQLLMSFTDALAEEVRGQRDAGRLPADLDPTATASVLVAMLAQVSGHRYGIERHGT
                     TGPELQRALARLLYSGVTGRKPPG"
     gene            13246..14010
                     /locus_tag="PLZ72_09025"
     CDS             13246..14010
                     /locus_tag="PLZ72_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36476.1"
                     /translation="MVDRPAGRRGLPALGVPVHRVAPGAPVTLDPVVRFWTATGGDVG
                     MIRVDRLPITSTSLWTRPVRPDPDHVPLQLHFDVKEALGFEHAVIATHRTVLHRPSRV
                     GDRVGHHQALRAIGPERMTALGRGRSWEVDLVLTDARGDLLQVETFSAFGYRPGTGSR
                     ASRPRGAGQGRQGDSPGVSGHRIRAAAAASRVWAPVHHDVVAARRAGFDGVIACTQHL
                     AAIAEQTRLREARPHGSVRVLEVRMGRPVVACREGS"
BASE COUNT         1975 a         5169 c         4964 g         1971 t
ORIGIN      
        1 cctctccatc caccacgctg tcccccgaca tcgttgcgct cgccgacgcc gacctccgcc
       61 agcatccacg tgacattagt cctactgctg gtagggattc atctgcgcgg gttgggtcga
      121 atcacccagc gctgggggag atgccgccgg tgcggccgtg gatgcccgtc gcggacggcc
      181 gcggcggctt caatggtgcg gtgctgttcc cgacgatgac gttcgcgatc ttcttcgcga
      241 tcgtcctcgg cgtcagctgg cggatcgcgg atcggcccct ggcctggcgt tggttcgtgc
      301 tggccgccag ctacgtgttc tacgggtggt gggacgcccg gttctgcctg ctgatcgccg
      361 gctcgaccct gatcaaccac ctggtggtca acctggtggc gcgggcgcgc accgaccggg
      421 cggcccgcgt ctcgatgatc gtcggggtcg gcgccaacct cgtcgtgctc ggcttcttca
      481 agtactacgg gttcttcgtc gactcgctgc tgcagatcct ggagccggtg gggctggcgc
      541 cgacggcgct gctgatcaag gtgggactgc cggtcgggat ctcgttcttc acgttctgcg
      601 ccatcagcta cgtcgtcgac gtctaccgcc aggagcagga accgatcgcg ctgctcgact
      661 tcgcggtcta cctgtcgttc ttcccccacc tcgtggccgg tccgatcgtg cgggtgtcgg
      721 agttcgagcc tcagctgcgg cgtcgacgca accgcgagcg cgtcgacgcg gtgcgcgccg
      781 cccgacttat ctgccgcggc atgttcaaga aggtcgtgat cgcgaactac ctggccaccg
      841 cgatcgtcga cccggttttc gcgtcgccgg gtcagtcgcc caacctcgag ctcgcggtcg
      901 ccgcggtcgc ctacgcggtg cagatctacg ccgacttctc cggctacacc gacatggcga
      961 tcggcatcgc gctgctgatg ggcatccgct tccccgacaa cttcgaccag ccctactcgg
     1021 cgacctcgat ccaggacttc tggcggcgct ggcacatgac gctgtcgcga tggctgcggg
     1081 actacgtgta catcccgctg ggcggcaacc ggggcggcga gcgcgccgag atgcgcaacc
     1141 tggtcgccac gatggccatc ggcgggctct ggcacggcgc caactggacc ttcgtggtgt
     1201 ggggcttcta ccacgggttc ggcctggtgg ccgagcgcaa gtggcgggag cggacgccga
     1261 ggtcctggcg gcgtggcgcc atcgacctgc gcaacccgga cgccgatgac gacaccgcgg
     1321 cggccgacgg ctacacccgt gtgatggccc gaccgacggt gcggcccgaa ccgaacgtgt
     1381 gggtctcgcg cgccgcggtg ttcgcgttcg tcaccgtcgg atgggtgttc ttccgggccg
     1441 tcgacctccc caccgccatc ggctacctgg tgggcctggt cacccggtgg ggtgtcggcg
     1501 acctggtcac cccgctcgtg ctggtgacga tcgtcgtcgg catggtcggt cagttcgtgc
     1561 cccgccgggt cggcgacgcc ctcgagtacc gggcgtcgca tctcccgccg gtgctcctcg
     1621 ggatcggagt cggtctgttc ctcgtggtgt gcaacctcct cgggccggtc ggggtcgccc
     1681 cgttcatcta cttccagttc tgatggatcc gacccccgcc gcccagcttc cgaacctgcc
     1741 gacagtgacc cgatgcgcgt gaccgacgac gaccgaggag ccgaccgggc cgcccgatcg
     1801 ggcccggacg cgccgaaggg ccgacccgcg tcccgaccgg gccgaccccg gcgtcgggtc
     1861 cgtcggacga tgtcggccac caccgcgtgg aagtcgtacc tggtcgcgct ggtcgtgttc
     1921 gccgtgctcg gctccggcgg tctgcgggag tccgccgagg ggatgccgct cgggtggccc
     1981 cgcgacgtcg ccatggcgct gaccggcgga ctcgaccggg cggtcaacct ggtgtcgctc
     2041 aaccgtccct acgactgggc cgccgagcgc ctcggccgga cccaggccga cgaggacttc
     2101 gagttcccgg tcccgacgac cacccccgac gccaccgcca ccaccacgac cctccccccg
     2161 ctgcggacac cgaccgcggc cgacccgctg aaggtcgtcg tggccggcga ctcgaccgcg
     2221 atcggcgtcg gtgaccgcct caaggtcgcc gtcgacgacg accccaccct ggccgtcgac
     2281 gtgcagggca aggtggcgac cggcctgacg cgctccgact acttcaactg gggtgcccga
     2341 accaaggagc tcatggagac cctgtcgccc gacgtggtcg tcttcatggt cggcgccaac
     2401 gacacccagg cggtgatggc tcccgacggg accgtcgtgg cccgctacgg cacgccgggc
     2461 tgggccgacg cgtaccgccg tcaggtggcg gggatcatgg acctggccca cgacgggtcg
     2521 cgccggttgc tgtggatcgg ccaaccccgg gtcggcaacg ccaagatcaa cgccacgctg
     2581 tcgcaggtca acgacatcat ccaggaggag gcggccggtc ggccgtgggt cacctacttc
     2641 gacctggcgt cggtggtggc gggacccggc gggtcgttct cggagtacgt cacgttcccc
     2701 gacggccaga ccgtgcactg cttcgccggt gacggcgtcc acctgagcat gaagtgcctg
     2761 gaccgctcga tgtccgagct cgtcccggcg ctgcgcgagc tctaccggac cacctgaccg
     2821 cccgggtccc ggcgacccct cagcccatcc cgaggctgtt gagcagcgga ccgttgagcc
     2881 acagggccat cagggcgccc agcgccagcg ccgggccgaa cgggaaccgc gtcccccggc
     2941 gggcgacgag cgagccgatc cccatggcca gtccgatgag gctgccgaac aggagcccgt
     3001 agatcggcag caccaggctg aaccagccga ggaacaggcc gagcaggaac atcagccgca
     3061 cgtcgccgaa gccgagcttg cccggggcga ccagccacag gacgaagaac aggccgaaca
     3121 tcacgacgcc gctgatgacg gcgcgcacga tggcgtcggg tgccccgatc accagcgaca
     3181 tggcgcagat cagcacgaag ccgaccgcgg cgccgaccca cggcatccac cacgggatga
     3241 gccagatgcg caggtcgatg atcgcgaccg cggccaacac gacgaccagc cacagatagg
     3301 ggagagcgac ccaacccgag ctggtggtgg ccaccgtcag gaccatggcg accgggacca
     3361 cgacctgcag cagcgggacg gtgaacgcca ggcgttcacc gcaggagggg cagccgcgga
     3421 accagctgag gaccgggacg acgtcggcgg cccggtagac gtggtggcag tgcgggcagc
     3481 gggcctggcg gtactcggcg cggatgaccg gcggtggctg gtcggcggtg ccgacctcgg
     3541 cggcgacgtc gcggggggtg agcgggcgga gctccttgta gcggtgcacg ggcaccgtga
     3601 gctgccatcc ggcgacgatg ccgatgacgg cggacaggac caggagcgcg gcgttcaccg
     3661 gttcccatcg gctggcggcg gacggtcctg aagcttcggc gagtcgggag gcccaccggg
     3721 tcgacgttcc cggtgtccgc gtgtgggatc gacgcgacgg cggtcgttct cgcacacgga
     3781 cgtcaggtgc cgatgaccgg ctgggccacc cgcaggcgag ggtcgtagtc ctggtcccac
     3841 ggcagccgac cgttgcggtc gggccacacg aactgcagca tccgccagcg gtcggcgccc
     3901 actccacgtc ccgcgtggaa cgcggacgcg tcggggaacc gcgccgcgtg cacggcgagg
     3961 tcgacggtga gcagggcgca cggaagatcg gactcgagca gaccgacgaa cacgccgtcg
     4021 accgggagca cgacgccgcc ccgatgctgg tccgcgatca tgccgaggag cccccgggcg
     4081 gccacggcct ggagcccgaa gatcacgacc tcgggacggt cgaaggcggt ctcgaagccg
     4141 atcgtgtacg tgtaccccgg ccgcggcggt ggtccggcgt cggcctcgac cggatccgcg
     4201 acccagccct ggtcctcgat cacccaggcc agcttgtcca ggtgctccat gttgcggtcg
     4261 gggaagacgt cgtcggtcac gtggggctcc gatcggcgac gatgccggcc agggccgccc
     4321 ggtcgagctt ggccacggtg gtcagtggca gtcgcggcac cacgacgatg tcctcgggca
     4381 gcttgtggcg ggccagccgc gtcgccgcgt ggtcgcggag gtcctgcagc gagggtgcgg
     4441 tcgccgggtc ccgggccacg acgacggcga cgccgatctc gcccatgacc tcgtcgggtc
     4501 ggggcgcgac ggccaccgcc gccaccgccg ggtggtccga caacgcggcc tccacctctt
     4561 cggggaacac gttgtagccc ccgcggatgt acatgtcggt gcgtcgaccc gacaggacgt
     4621 agcagccgtc gtcgcgacgc acggccaggt cgccggtgcg cagccagcgg tccgtcgtca
     4681 acgccgccgc ggtcgcatcg gggtcgttcc agtagcggtc catcaccgcc gcggagcgga
     4741 tctgcagctc gccgacctcg ccgtcgggca gcggaccgtc gtcctcgtcg gtgaccctcg
     4801 cctcgactcc cgctcggggt cggccgatcg tgtgcagcgc ctcgtcgtca tccgcgtcga
     4861 acgcggtgcc gagtccgacg ccgccggact cggtcgagga gtagcggatc gagtaggccg
     4921 cgccgaagcg ctcgcgggcc tcctgcacga gcccgggcgg cgacgcggcc ccgccggcga
     4981 tgatcgccga gaccgatccc aggtcgaggg actccatcag cggtgagcgc agcatgagcg
     5041 cgagctgggg agcgaccacg ccgatggtgg gcatccggtg ctcggcgacc aggcgcaggg
     5101 cgtcgtcggc ccgccaggtg tcgaggacgt gcagggtcga accggtccgc aggtaccagg
     5161 gcagcttcaa cgccatgccc acgtgggcga actgggtcga ggccaacatg gcggcgccgc
     5221 cgccccaacg ctgctcggcg ccctcgccca ggtcgaggtc ctgcaccgcc cgctggtggg
     5281 cgacggtgaa caccgctccc ttggcccgcc cggtggtccc ggaggtgaag cagatcgtgg
     5341 tgacacgacc ggggtcgtcg gtcgtcggac cgggccggtc gccggtgtcg ggcgtcgtcg
     5401 gtctgcgggg accccacagc ggctcacccc ggtcgccggg ctccaccacc acgacgtcgg
     5461 accgcaacgg cagaccctcg acgccggcac ggtcggtgac caccaggtca ggggcgacca
     5521 gggcgaccag ttcggccctc tcgggcgcgg tgagcttggg gctgaccggg gcgtggacgg
     5581 cgccgatccg atcgagggcg accgcgagga ccacccactc ggcacacgac ggcagcacgt
     5641 cggacacgac gtcgccggga ccgatccccc ggtgaaccag gccggcagcg acgcggtcgg
     5701 ccgccgcgtc gaggtcgcgg taggtcagcg cgtcggtcgc gcggcgcagc acctctcggt
     5761 caccgaagcg gtcggccgcg gtccgcacga tctcgccgag cacgccggga gcttacgggt
     5821 gccgcggcac cgacgggacc ggcacagaag atgacgaaac gatgtccatc ccgtaatggg
     5881 ggtgcaacat ttcccccgca gatcgcgaga cagcaccccg aagcccgctc aaggatggag
     5941 gaccccccgt cgatacccgg gacacagcac cccgtcgtcc cacccctcag gacccgatct
     6001 ccatgtcacg agaccgacat ctccgccgct cccgttccgg atccccgacc ggtcgccgtg
     6061 ccctcggggc gctcaccctg atcggagtcg ccggcctgct cgcggcctgc gcccctgcct
     6121 cccggaactc gtcgggccac ggctcgacgc cggcgctgga cgcgtcgcgg gcgcccgctc
     6181 ccgctgcacc cgtcacgacc cgtccgacga cctacgtcac cgcaggtgcc cgagcgggga
     6241 tcggggggtg cgacctgttc ccggtcgacc acgcgttcca cgcgtcgatc gcatcgctgc
     6301 cggtgcgcag cagcagcggc gcgaccatcg cggcgatcgg gccgtcggag accctgctcc
     6361 ccgggttcag cgccgcgacg tggatgggct cgcgctcggg gtacccgacc aacctgatcg
     6421 actcccgcac cgctccccgc tccttcgtcg cggtcgcctc gatgtaccag tcggtgtcgg
     6481 agaaccagtc ggttccgtgg ccggacgacc cccgcttcga ggggtggccc gaccgggcct
     6541 gggaccggca cctgctcacc gtcgacaccc accgctgcga gtcctgggag ctgatcaacg
     6601 cgcaggcgcc cggcgagaac gtgttcgcgg cgctcctgca cccgggcagc tggtacgcgg
     6661 accgggtcgt ccgcctcgac ctctcgtcca acacgatccc cccgacgggg acggtgaccg
     6721 cgtcgggcct gtcgatgctg gcgggcctcg tccgctacga cgaggtcgcc tcgagtcgcg
     6781 tcgaccacgc gctgagcatg gtgctgcccg acatcggcgc cggcgcgacc tggccggcca
     6841 gccgctcgga cggacgttcg accgatccgg ccgctccgcc gatggggacg tggttccgcc
     6901 tgcgcgccga cgtcgacatc tcgggcctgc gcccccaggc ccgggtcgtc gcccaggcga
     6961 tgaaggacca cggcgtggtc ctggtcgaca ccgggcccca cgcggcgatc tccggcgaac
     7021 ccgacgtccg ctgggacgac gccgatctcg acggcctgcg gtcgttgacg atcgggcagt
     7081 tcgaggtggt cgacccgacg ccgatgaagg tcgccgacgg gagctaccgc atccgttgac
     7141 ctccgctacg atctgacgac ccgtcagatc ccgcgccgga gccggcgcgt ccggaggaac
     7201 catgcccgcc gaagccgacc ccgtccgacc gagcccgagc ggcgccgcgc ccctcgccgg
     7261 ggtccgggtc atcgaggtgt ccctgctcgg cccgggcgcc gtcggcaccc acctggccga
     7321 cctcggcgcc gaggtcatca aggtcgagcc gcccgcgggc gactacatcc gccagatgac
     7381 gtggccgatc atcgagggca gctcgctgct gcactggcac atccaccgcg gcaagaagtc
     7441 cctcacgctc gacctgcgca ccgacgaggc ccaagacgtg ttccgggacc tcgtgcgcga
     7501 cgccgacgcg gtcatcgagg ccatgcggcc cggtgccctg gcccgacgcg gcctcggcta
     7561 cgaggacctg ctcgagatca acccccggct cgtgttctgc accatctccg gctacggcat
     7621 gaccggcccg tacgccaacc tccccagcca cggcatcgcg tacgacacct gggccggcat
     7681 cgtgaacccg acattcaacg acgacggctt cccgtacctg cccgagcacg cctcgatcgg
     7741 catccacgcc ggaccgctgt tcggagccct cggcgtgctg gccggcatca ccaaggcgcg
     7801 ggccaccggc gaggggtcgt tcctggagat cgcgcagtcc gacgccgcgg cggccatgga
     7861 ctggctgcgc agcgagacct ggaaggccta cgagcggccc gagtccgagg tcaccggcaa
     7921 caaggccgac gactacgagc gtcgtgcccc cggcaccgcc ggcatggaag agggcgtccg
     7981 ctaccaggtc tacgagtcct ccgacgggtt cgtcctgttc atggcctccg agcaggagtt
     8041 ctggaagaac ttctgcacgg gcgtcgatcg gatggacatg ttcgagcggt ggcccggcaa
     8101 cacctacggc gaccacgccc ggggcaacac cgagatgcgc cgcgagctgg cggcgatctt
     8161 cgccacccgc accaccgccg agtgggtgga gtggtccggc accgtcaaca cgacgatcgc
     8221 cccggtcaac acgccccaga ccctggccga tgacccccag ttcctcgacc ggttcgggtt
     8281 catgccgcac gagacccacg gcgccgacat gatcggcacg ccgctcaagt tcctcgacgc
     8341 cgagctgccg gatcccaccc cggcgccgac cgcgggtcag cacaacgacg aggtgctcgg
     8401 cagcgtgctc ggctacgacg gcgagcgcat cgccgccctg cgggcggcgg gcgcgctggg
     8461 cgagggcaac tgagccgaat cccgggccgc accgggggct ccgctgatcg ggtcaggggg
     8521 agacatgacc gcaccgatcg tggtggaacg cacgagccgc ctggcggcgc caccgggtcc
     8581 ggtgtgggcc cacgcctcgt cgatggcagg cgtcaacctc gagctcgccc cgttcgtgcg
     8641 catgacccac cccacggacc gcgatcggtt gaccggcccc caggtgagct tcaccagctg
     8701 gctgctcgcg ttcgggctcg tgccgtccga ccggcactcg ttccggttgc tcgaggtggc
     8761 cgactccggc cgcgagggcg gatcgttccg cgaggactcc cgcagctgga tgcaggcccg
     8821 gtggcgacac gaccgcgacg tggtcgccga aggctccggt tcccgggtca ccgatcgtct
     8881 ggaggtcacg ccgcgactcg cggtcgcccg gccgctcgtc gccgctctcg tcgggttcct
     8941 gttcacccac cgccaccggc gcctgcgcga ccggttcggc gaggagcgcc ccggcccgga
     9001 tcgagccgct cgggcgcccg gtacagtcgc cggcgatgca ctcggcccgg cgcagcaaga
     9061 tgacgtcgtg acggaggacg gacccatgtc gacagacgca acgactcccg gtgaggccgc
     9121 cggcgacggc acccgcggcg acctccgctg ggcgtcggtg ccggcgctgg tgcgcgacgc
     9181 cgcggagcgc cacggcaccg ccgaggcggt ggtggacctc gccggtgacg agccggtgcg
     9241 gctcgactgg gccgagctgc gccgccgggt cgacaacatg gccaagggcc tgatcgccgc
     9301 gggcgtcgag cccggtgacc gcgtggcgat ctgggcgccc aactgctggg agtgggtggt
     9361 cgcggtcctc ggcctgcagt ccgccggcgc cgctctcgtc ccgctcaaca cccgctacaa
     9421 gggcctggaa gccgccgaca tcctcgcccg cagccgggcc cgcctgctgc tcaccgtcga
     9481 ggggttcctc ggcaacgact acgtgtccat gctcgacgcc gccggacgcc ccgacaccct
     9541 cgagggcatc gtcctgctgc gcgacaccgg ggcgcggccg gccggcgcga tcaccctcga
     9601 cgagctcctc gccggcggcg acgcggtgcc cgacgccgag ctcgacgccc ggattgccgc
     9661 gctcgacggg aacgacgtgt ccgacgtgct gttcacctcg ggcacgaccg gcgcccccaa
     9721 gggcgtggtc cagacccacg ccgcgacgct gcgggcattc ctcgactggt cgaccctcgt
     9781 cgggcttcgc gctgaggacc gctacctgat catcaacccg ttcttccaca ccttcggcta
     9841 caaggccggc atcctggcgt gtctgatgac cggggcgacc atggtcccgt tgccggtgtt
     9901 cgacgtcgag gccgccatcg acgccatcga ggccgagcgc atcagcatga tccccggacc
     9961 gccgacgctg taccagacgc tgctgaacca cccccgcttc gaccggtccc gggtcgacac
    10021 gctgcgcctg gcggtcaccg gcgcggcggt ggtgcccgtc gagctcgtcg tcgccatgga
    10081 ggagcagctc gggttcgaga ccgtgctcac cgcctacggg ctcaccgagg cgtgcggggt
    10141 ggtcaccgtc tgccgacgcg aggacggcgc cgagacgatc gccacgacct ccggtcgggc
    10201 catccccggc gtcgaggtcg tcatcgcgga tcccgacggc aaccggctcg gcccggacca
    10261 gcccggcgag gtcctggtgc ggggctacaa cgtcatgcgg gagtacttcg aggacccgga
    10321 gcagaccgcc gaggccgtcg accccgacgg gtggctgcac accggcgacg tcggcacgct
    10381 cgacgcccgc ggctacctgc gcatcaccga ccgcaccaag gacatgttca tcgtcggcgg
    10441 gttcaacgcc taccccgccg agatcgagca gctcctcggg gcccatcccg acatcgccca
    10501 ggtggccgtc gtcggcgtgc ccgacgagcg gatgggagag gtgggcgtcg cgttcgtggt
    10561 gccgaccgcc ggtgccgagc tcgacggcga tgcggtcatt gcctgggccc gggagcacat
    10621 ggccaactac aaggtccccc gccgggtcga gatcatcgac gccctaccgc tcaacgccag
    10681 cggcaaggtc ctcaagttcg aactacgcga ccgggccacc tccgggtcct gacaggagct
    10741 gtcccatgaa gttctcccag gcgcttctcg gcgccaaccc gctcgactgg ccccgcatcg
    10801 cggctgcggc cgaggaggcc ggcttcgact cggtcgccgt gtcggaccac gtcgtgtacc
    10861 ccgcccggct cgactccagg tacccctaca cgcccgacgg cgtcccgctg ttcagccccg
    10921 acgaggactg gccggacccg tgggtggcgg tcggggcgat gtcggcggtc accacccggc
    10981 tgcgcttcct gaccaacgtg tacgtgctgc cgctgcgcaa cccgttcgtg gtggccaagg
    11041 cggtcggcac cgcggcctac ctgtcccgcg gtcgggtcgg cctcggcatc ggcgccgggt
    11101 ggatggccga ggagttcgag ctgatgggcc agcccttcgc ccggcgcggc aagcgcatgg
    11161 acgagatggt cgaggtggtc cgcaccctgt ggcgcggcgg catggtcgag caccacggcg
    11221 agttctacga cttcgacccg gtggagatgc gcccggctcc acccgccccg gtgccgatct
    11281 acgtcggagg tcactccgag atcgccatgc gccgggccgc caccctcggc gacggctggc
    11341 tcggcatgta ctactcggtc gagaagctcg agcagcactg ccggaccctg gaacgggccc
    11401 gtgaggacgc cggcaccgcg gaccgtccgt tcgagatcat cgcctcgccc atggtcacgc
    11461 cgaccgccga gaactgcgaa cggctcgagg cggccggggt gacgaccatc ctgacctcgg
    11521 cgtggatggc ccagggggtg ctcggcccag acagcaccga gcaggcgatc gagctgatcg
    11581 ccacgttcgg cgagcggttc atcgcctgac ccgctcggac ctcgggcgcc ggcgaccagt
    11641 gagaggtcgc cgtcgcccga gatcgcgctc agctcgacag cgggagcgcg tggacccgga
    11701 gcggacccgg gggagaatgg gggatcgtga gcgacgacgt ccgccacctg ggcaccatcg
    11761 agggccggga gatccatggc gcatccggcc ccgccgcgcc ccccgaacct ccgcccccgc
    11821 gggccggtgg cgcacccgtc gtgcatcccg gaggtccgcc cgcacgcccg gggcggaccg
    11881 tggcggtccg tgccgaggtg gccgaggcgc tgcacgacat ccgcgacgcc caccgccgag
    11941 gtccgaagcc caacacccgt cccctgccgc gctgggtgga gcggttggcg tgggtgctcg
    12001 acgacgccat cgagatcccg gccaccggcg gtcgtcgcgt cggcgtcgac gggttcatca
    12061 ccctggttcc cggcatcggc gacgcggtcg gggtggccct gtcgatggtc gtcgtctccg
    12121 ccggtgtcct ggccggtgtg agctggccga cgatcatccg catgctgtgg aacgtcgcct
    12181 tcgaaggggt ggtcggcctg atcccgttcg ccggcgtcgt cttcgacatg gcctacaagg
    12241 ccaacgaccg caacgtccgg ctcatcgagg ccgacctggc cgaccgcgcc gccacccgcc
    12301 gctccagcct gggcgtgatc ctggcgctgg tgctgaccag catcgtcggg ttcgtgatga
    12361 tggtcgtgct gagcctgctg ggcatcacgg tcatggtgtg gatcctctgg aagctgctcg
    12421 gcggctgagc accgccggta cgctgccctc ggtcggtgac ggccctcggg gcgggtccgc
    12481 cccggaccga tcacgacgaa ggacgcggga gtggcaacga agagcgcaac ggagcaggtc
    12541 gcagccgagg gctcggcgac cgatccgacc gatccgaccg atccgaccga gagcgacgag
    12601 ctgcgggcgg tcgacgggcg ggtgcccggt cgacgcgggc tggccacgcg ggcccgactg
    12661 ctcgagcaga cccgggagct gctgtacacg acgtcgtacc gggacctgaa ggtggtcgac
    12721 atcgcccggg gcgcggggac atcgccggcc accttctacc agtacttccc cgacgccgag
    12781 tccgccctgt gggcgttggc cgacgagctg gtcggcgagg gtcgtgagcg cctgacccgg
    12841 ccggtgcgcg acgggagctg gaacgggcgt gcggcctacg acacctgcga gcgcatcgcc
    12901 gcgagcttcc tcgagttctg ggacgagcac cgggcgctgc tcgcggtcat cgacctggcc
    12961 gccaccgagg gcgacgtccg gttccgcgag atccgcctcc agctgctgat gagcttcacc
    13021 gacgccctgg ccgaggaggt ccgcggccag cgcgacgcgg gccggttgcc ggcggacctc
    13081 gacccgaccg ccaccgcgtc ggtgctggtg gcgatgctgg cgcaggtctc ggggcaccgc
    13141 tacggcatcg aacgccacgg caccaccggt ccggagctcc agcgggcgct ggcccgactt
    13201 ctctactccg gggtgaccgg ccgcaaaccc ccgggctgat ccgacgtggt cgatcgtccg
    13261 gccggccgtc ggggcctgcc cgccctcggc gtgccggtcc accgggtcgc tcccggggcc
    13321 ccggtgacgc tcgatccggt cgtccggttc tggaccgcga ccgggggcga tgtcgggatg
    13381 atccgggtcg accgactgcc gatcacctcc acctcgctgt ggacacgtcc ggtccgtccg
    13441 gaccccgacc acgttcccct gcagctgcac ttcgacgtga aggaggcgtt gggattcgag
    13501 cacgccgtca tcgccaccca ccggacggtg ctgcatcggc cgagccgggt cggtgaccgc
    13561 gtgggccacc accaggcact gcgcgcgatc ggcccggagc ggatgaccgc gctgggacgg
    13621 ggccgttcgt gggaggtcga cctcgtgctc accgacgcga ggggcgacct gctccaggtc
    13681 gagaccttct ccgcgttcgg gtaccggccc ggaaccgggt cgcgcgccag ccgcccccgg
    13741 ggtgcgggcc agggtcgaca gggcgactcg ccgggtgtgt ccggccatcg catccgcgcc
    13801 gcggcggccg cgtccagggt ctgggctccg gtacaccacg acgtggtcgc ggcccgtcgg
    13861 gccgggttcg acggggtgat cgcgtgcacc cagcacctgg cggcgatcgc cgagcagacc
    13921 cggctgcggg aggcccgccc gcacggatcg gtacgggttc tcgaggtcag gatgggtcgg
    13981 ccggtggtgg cctgccgcga agggtcctga accccgcttt gtcctgcacc agcgcggtga
    14041 tcaccgcgct ggtgcatgac aaagcggggg tgtggcggc
//
LOCUS       DAOQOD010000078        13681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1591_length_13681_cov_8.257596, whole genome shotgun sequence.
ACCESSION   DAOQOD010000078 DAOQOD010000000
VERSION     DAOQOD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13681
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_13681_cov_8.257596"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLZ72_09030"
     CDS             complement(<1..735)
                     /locus_tag="PLZ72_09030"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNA36477.1"
                     /translation="MAVPRSLMSPDPRARPAASVTVDRVRVTVLTPRLLRLEHAADRQ
                     FEDRGSTAVVNRRFTVPDFEVREHAGGFVLDTGEVTLTVTDASRPLTRSTTSARIGRG
                     RQSVTWRPGDRARTNLGGTARTLDNCRGREVRELVDLDPDGELVFSDWTRWDLDPGLL
                     SRDGWVVLDDSTSPVFDPDAGRPEPARGRGWPTARPAGRRQDLYLFAHGTDHRGALAD
                     AAALIGPQPLPPRYAFGYWYSRYWAYT"
     gene            853..1272
                     /locus_tag="PLZ72_09035"
     CDS             853..1272
                     /locus_tag="PLZ72_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA36478.1"
                     /translation="MPAPVFTGIAHLDLSVSDIDASAEWYEKVLGLRNLHRAELDGRT
                     MVVMLAGAARMIIGLNQHDGRPDGRFDERRVGLDHVGFEVPERADLDEWQRHLAALDV
                     EHSPVADTDVGSALVFRDPDGIQLEIWWNAPRRPKES"
     gene            complement(1321..3936)
                     /locus_tag="PLZ72_09040"
     CDS             complement(1321..3936)
                     /locus_tag="PLZ72_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNA36479.1"
                     /translation="MAERAASPIGTFRKAGVAILAVSLVAVLAACSSSDDDESAPTTV
                     GSGDRYAATIRRTTGGVPHITGKTLADASFGEGWASGQDRACDLADQVIKVRGERARW
                     LGAGEDDANVDSDAAWRAIGIHERAVKDWEEVSEELNEQITAYTAGWNAHLKAVGAKG
                     VKGWCSGAAWLKPLEPVDVYAYARSIALTASSARLARYIPTAHPPTETASVSPDASGP
                     GTHTTLRLASTQDPAAPSPATGPVVDAAAIGSNGWAIGKDRSADGRGMLVANPHFPWE
                     GELRFWEAHLTVPGQIDAYGVTLSGLPGLGIGFTKTFGWTHTVSAGNRFTAYRLSLVP
                     GQPTKYRYGDQVRDLVPSEHAIEVLGDDGKVSTVTRTTWSSHYGPVLDFPGVGWTDQS
                     TITYRDANIDNAAFAEQYLAFLKAKDLDEFIAAGKKINGVPLFNTIATSNDGRAYYAD
                     WSATPNLSPEAIAAYDASLASDPIVKIAADSGAVLLDGSDPRFEWVDAPGARSPGLVP
                     SDRQPAIERPDYVFNANDSFWMPNAEVMLDGDYTPLFGRQKTPRSPRTRENAVVLSDT
                     ADTGPAGEGGLFTLDSLADASLLNQGYTARELLDDVVQRCTATPSVTVPELPAETPNP
                     AATPDTPPPAPGLPAATVDISAACKVLGDWDGIYDLDRKGPAIWRETMSRYKGNDFLN
                     AGALWATPFDASDPVNTPNGLAPAAGGTDQVLVNLARAVQTLDAAGIPLDATLGETQF
                     AMRDGKRVPLHGGTGVDGVTNVVTWGSLTSTSDPVVRDLKREKVAAGSTLNRLDGETG
                     YPVNFGTSFLLALAYTDDGPTAKAFLTYSNTENPKNPDYVTATERFSAKKWRKVAFTE
                     KQVEADTTSTVNVRG"
     gene            complement(4082..5680)
                     /locus_tag="PLZ72_09045"
     CDS             complement(4082..5680)
                     /locus_tag="PLZ72_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNA36480.1"
                     /translation="MGTRTTIEVPADEWLGVGPLVTFAAPDEFAVLGMLRMVGDGARR
                     YWWSGDGRRVVRQRGGTDDGTYDILLSPRLVEFAFGRALTGEAATIDLVTDDDGSVLA
                     VTARTDTQALTVAADPRAYPSIDEVFASELAADGAWAEVDAEELAALIDVARRRPAVE
                     HIDDEEPDPLFWLAVVGGEGIMIDLRWPGLGLTEFQLRARAEGSRTAAVPPSQLADAL
                     IGLEGPITVVVPDFAHNAIRVSSADRDALILPIETTFERMRFSTEQVLAEVFGPTVVQ
                     RDTDGHYRLSHDIPVFARLVDDEPVRIQVFAVAVDGVEHSAELLSELNDHNARLGFAR
                     CFWMNDQVLVECDLMAGTTEAAELQAAHERVLTISRELGPLLAAVFGGTPAETHVGGA
                     GSGNWDDYLRTVVAAELVDGTLTPISDADAWPYPGTVWALTSDNPMGTWRSDEENQAA
                     RPELIAAVDAAGARHHRSVGTSVDTEHSEVGLVVWDTDRDTVLDIARRFRQEAIFEID
                     PDEVRVIACFDDRAAARPRGLLLR"
     gene            5838..6494
                     /locus_tag="PLZ72_09050"
     CDS             5838..6494
                     /locus_tag="PLZ72_09050"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNA36481.1"
                     /translation="MGEAPTGPDHGPVLRYGDGQRALQDRFDTRRLADRLAETATDDV
                     TGYRSFIESLDTFFLATVDEHGQPQCSHKAGDPGVVRVIDGHTLAFPSYDGNGMFLSL
                     GNLAANPAVGMLFIDFEGGTRLRVNGIASVDLDDPLTAEFPCAQAVVRGRVTAAFPNC
                     RRYVHPRRRVERSPFVPTGQDDPPVPDWKLIPWFDGHLAADDPALDPQRPSAPATPEF
                     "
     gene            complement(6504..7859)
                     /locus_tag="PLZ72_09055"
     CDS             complement(6504..7859)
                     /locus_tag="PLZ72_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNA36482.1"
                     /translation="MLWRGKAIAVVACVAVALVAGSCTNNPTGGGMAPTGSGCMSPPV
                     IGPGASLYNCSLIGLDLSGVDLSGADLRGSDLTGADLSGANLTNANLSGANLTGANLT
                     GADMAGAILSGAILLGALFLGANLLGAVFDFGGFTPPAGGSTGGGGAVGTPVGACTGP
                     YCAGYNEATVDTGVDVCGETYGADLGVPSFYLHQDNDTLAANGQRSVVTDAATDFTGA
                     VLGGTQFAFRGLSLSKANFTNAVIHGVSVGCQDGSGGRFDGATFVGGELIDITLPNTT
                     AVGATFVDLHMCSVNLSDSDLDGAKFRGETGSTCTDIMNAVPSRFSAIVMFERSSLIG
                     AQFGDPALLSEDPAGTAPNGWYRSAWVNYTGDGKPSVTFQDADLTSASATYPEFRNAR
                     FTGATLSSFVSQAQAQPALYDSVDFTTADWTGATFTGPHDYAGSTCPDGTVAGPGDAC
                     F"
     gene            complement(7951..8325)
                     /locus_tag="PLZ72_09060"
     CDS             complement(7951..8325)
                     /locus_tag="PLZ72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA36483.1"
                     /translation="MIDHLGIQCSDVAASAAFYDRVLAPLGASRLMEPHPGVIGYGTT
                     QPDFWLGPLSDSHTGWREDHIAFQAPDRAAVRAFFDAAVALGAEVLHEPREFPEYHPG
                     YFGAFVRDPDGNNVEAVCHAPE"
     gene            complement(8322..9395)
                     /locus_tag="PLZ72_09065"
     CDS             complement(8322..9395)
                     /locus_tag="PLZ72_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA36484.1"
                     /translation="MPPTTIELGIDTFGDMTVDPDGVPQHPARVIRDVVDQAVLAERV
                     GVDVIGLGEHHRDDFALSAPEIVLAAIAARTERIRLSTAVTVLSSDDPIRVYERFATL
                     DAVSNGRAEIIVGRGSFTESFPLFGFDLRDYEVLFDDKLSLLAELLTEEPVTWSGRTR
                     PPLRDQVVHPRTESGRIRTWVGVGGSPESVVRAARHRMPLMLAIIGGEPARFAPFVDL
                     YHRALGEFGHDSLPVGCHSPGHVADTDEQAIEEAWPHHRVLHGRIAAERGWSPMTRSD
                     FEAQVGPRGALYVGSPETVARKIAATSRVLGLSRFTLKIGSGPMPDALQRHSIELFGA
                     EVIPRVRELLADGASQPPGTMAP"
     gene            9517..10101
                     /locus_tag="PLZ72_09070"
     CDS             9517..10101
                     /locus_tag="PLZ72_09070"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HNA36485.1"
                     /translation="MTAPAGYTYDDPSLSPSPVTLADLDDLLASVLWSEADAAALRRA
                     GEILVPQTEAILDVWYGFVGSNPHLVATFAGSDGTPDGDYLAAVRGRFGRWIEDLCRR
                     DFDAGWLAYQDEIARRHHTSGKNRTDSVDSPSGHVPLRHLFALVVPITVTIRDFLASG
                     ATDEVELDAMYQAWFKAVTLSATLWARPYSPDLW"
     gene            10155..10715
                     /locus_tag="PLZ72_09075"
     CDS             10155..10715
                     /locus_tag="PLZ72_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA36486.1"
                     /translation="MPERVVAALDRIARGVRAHRQTVATDAGVTVLQADLLRALHHGP
                     PPPALAGPLAADLGVSQPTVSDSLAALERKGLVERRSTPGDRRRSTFALTPDGDRLAG
                     GLRRADDVLADTVAALDPDDQEHVLRGLLDMITGMLDNRILAVARTCPTCRYFEAGTD
                     GPRCSLLQVPLAPGDLRVDCPDHEPV"
     gene            complement(10762..12108)
                     /locus_tag="PLZ72_09080"
     CDS             complement(10762..12108)
                     /locus_tag="PLZ72_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA36487.1"
                     /translation="MTDRLVVIGGDAGGMSAAAQARRLRPDLEIVVFERGEFTSYAAC
                     GLPYFVGGEVTDRDRLVARTPEEFRTRGIDVRTRHEVTAVDTAARTVEVRPLDGGGTT
                     IEPYGELLIATGASPVKPPLPGIDGPGVFDLRTIPGADAVRAAIDAGARRAVVIGGGY
                     IGVEVAEAFAARGLEVTLVELADQPMLTLDADMAGLVTTALEELGVKVLLGTGVTGFV
                     HEGGRLTAVDTTAGEIPADLSVVGLGVRANSALAAAAGIPVGASGGIVVDDRLHTQVD
                     GVWAAGDCVESHHRITGERVVIALGTHANKQGRVVGSNIGGTPSTFGGVLGTAITKVG
                     STEVGRTGLSEREAAAAGLDAFAVTARAAVRAHYYPGADPITVKLVVERGTGRLLGGQ
                     IVGGSGSAKRIDVVATALWNRMDVRDVAGMDLAYAPPFSPVWDPVLVAAGKAALQL"
     gene            12211..12672
                     /locus_tag="PLZ72_09085"
     CDS             12211..12672
                     /locus_tag="PLZ72_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA36488.1"
                     /translation="MRLNSYEVDPGGHKRLIGWAEHLDRGILPKELRALVETRVSQIN
                     GCAFCLAMHTDEGRTAGLPQAKLDTVAAWRDDPTFTDRERAALDLAETMTRIADGGRV
                     SDDVWDAAAAAFDEAELASLVQVVAIINAFNRINVATERGSNHYLEYASHR"
     gene            complement(12730..13137)
                     /locus_tag="PLZ72_09090"
     CDS             complement(12730..13137)
                     /locus_tag="PLZ72_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36489.1"
                     /translation="MSPLSALHHTRVRAALGPDASPRARPATVTDTAADGVANVRFLD
                     GDTSTLSVADSVRLAATLDRPDLCRLRGEPLVLWSAQHGVLAVATGPTSPPDRLVVQL
                     VSRVEDGSVVELIGGDDQPSWQVFAASGAIPAR"
     regulatory      complement(13144..13232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1903 a         4715 c         5074 g         1989 t
ORIGIN      
        1 ggtgtacgcc cagtagcggc tgtaccagta gccgaaggcg taccggggtg gcagcggctg
       61 cggtccgatc agcgcggcgg cgtcggccag cgcgccgcgg tggtcggtgc cgtgggcgaa
      121 caggtacagg tcctggcggc ggcccgccgg acgcgccgtc ggccagccgc ggccccgggc
      181 cggctcgggt cgaccggcgt cgggatcgaa caccgggctg gtcgagtcgt cgaggaccac
      241 ccagccgtcg cgcgacagca ggcccgggtc caggtcccaa cgggtccagt cgctgaacac
      301 cagctccccg tcgggatcca ggtcgacgag ctcgcggacc tcgcgacccc ggcagttgtc
      361 gagcgtgcgg gcggtgccgc ccaggttggt gcgggcccgg tcgccgggcc gccaggtcac
      421 cgactgtcga ccgcggccga tccgggccga ggtcgtcgag cgggtgagcg gtcgggacgc
      481 gtcggtcacg gtcagggtca cctcgccggt gtcgaggacg aagcccccgg cgtgttcgcg
      541 gacctcgaag tcgggcacgg tgaagcggcg gttgaccact gcggtgctgc cacggtcctc
      601 gaactgccga tccgcggcgt gctccaggcg gagcagccgc ggggtgagca ctgtcacccg
      661 gacccggtcg acggtgacgc tggctgcggg acgagcccgc gggtcggggc tcatcagcga
      721 acggggaacg gccatggctc ggacggtacc gccggagggg cgcggcgggc ggcgactcgt
      781 aggctcggtg gcctcagccg ggtcgtcgcc ggaggtcccg tcggaacgga cccgccgagg
      841 aggaggatcg cgatgcctgc gcccgtgttc accgggattg cccacctgga cctgtcggtg
      901 tccgacatcg acgccagcgc cgagtggtac gagaaggtcc tcggactgcg gaacctccac
      961 cgagccgagc tcgacgggcg aacgatggtg gtcatgttgg cgggagcagc ccggatgatc
     1021 atcgggctca accagcacga cggtcgcccc gatggtcgct tcgacgaacg gcgggtcggt
     1081 ctggaccacg tcggcttcga ggtgcccgag cgggccgatc tcgacgagtg gcagcgccac
     1141 ctggcggcgc tcgacgtcga gcactcgccg gtcgccgaca ccgacgtggg gtcagccctg
     1201 gtgttccggg accccgatgg catccagctc gagatctggt ggaacgcgcc ccgtcgaccc
     1261 aaggagtcat gaccgggacc gcggccccga ccggaacgac gccgggccgg ggcagcggcg
     1321 tcagcctcgc acgttcacgg tgctggtcgt gtcggcctcg acctgcttct cggtgaaggc
     1381 gaccttgcgc cacttcttgg ccgagaagcg ttcggtggcc gtcacgtagt cggggttctt
     1441 cgggttctcg gtgttgctgt aggtgaggaa cgccttggcc gtcggaccgt cgtcggtgta
     1501 ggcgagcgcc agcaggaacg acgtcccgaa gttgaccggg tagccggtct cgccgtcgag
     1561 ccggttgagg gtcgagccgg ccgccacctt ctcgcgcttc aggtcccgga cgacgggatc
     1621 cgaggtcgag gtgagcgacc cccaggtgac gacgttggtg acgccgtcga ctccggttcc
     1681 gccgtgcagc gggacccgct tgccgtcgcg catggcgaac tgcgtctcgc cgagggtggc
     1741 atcgaggggg atgcccgccg cgtccagggt ctgcacggca cgtgcgaggt tgaccagcac
     1801 ctgatccgtg ccgccggcgg ccggggccag cccgttgggc gtgttcaccg gatccgaggc
     1861 atcgaacggg gtcgcccaca gcgcgccggc gttcaggaag tcgttgccct tgtagcggct
     1921 catcgtctct cgccagatcg cgggtccctt gcggtccagg tcgtagatgc cgtcccagtc
     1981 gcccaggacc ttgcacgccg cagagatgtc gacggttgcg gccggaagtc cgggggccgg
     2041 cggcggggtg tcgggcgtgg cggccggatt gggcgtctcg gccggcagct cgggaaccgt
     2101 cacactcggc gttgcggtgc agcgctgcac cacgtcgtcg agcagctcgc gggcggtgta
     2161 gccctggttg agcagcgacg catcggcgag cgagtcgagg gtgaagagac cgccctcgcc
     2221 ggcgggtccc gtgtcggcgg tgtcgctgag cacgacggcg ttctctcggg tgcgtggcga
     2281 acgcggggtc ttctgacgcc cgaacagcgg cgtgtagtcg ccgtcgagca tcacttcggc
     2341 gttgggcatc cagaacgagt cgttggcgtt gaacacgtag tccggtcgct cgatggccgg
     2401 ctggcggtcc gacgggacca gcccggggct gcgcgcccca ggggcgtcga cccactcgaa
     2461 gcgcggatcg gagccgtcga ggagcaccgc tccgctgtcg gccgcgatct tgacgatcgg
     2521 gtccgacgcc agcgacgcgt cgtacgcggc gatcgcctcg ggtgacaggt tcggcgtggc
     2581 ggaccagtcg gcgtagtagg cgcggccgtc gttcgaggtg gcgatggtgt tgaacagcgg
     2641 cacgccgttg atcttcttgc cggcagcgat gaactcgtcg aggtccttgg ccttgaggaa
     2701 cgccaggtac tgctcggcga acgcggcgtt gtcgatgttt gcgtcgcggt aggtgatggt
     2761 cgactggtcg gtccagccga cgccggggaa gtcgagcacc gggccgtagt ggctcgacca
     2821 ggtggtgcgg gtgacggtgc tgaccttgcc gtcgtcgccg aggacctcga tggcgtgctc
     2881 gctcgggacg aggtcgcgga cctggtcgcc gtagcggtac ttggtcggct gtccggggac
     2941 gaggctcagg cggtaggcgg tgaagcggtt cccggccgac acggtgtggg tccagccgaa
     3001 cgtcttggtg aacccgatgc ccagtccggg cagacccgac agggtgacgc cgtaggcgtc
     3061 gatctgtccg ggcacggtca ggtgcgcctc ccagaagcgc agctcgccct cccacgggaa
     3121 gtgcgggttg gcgacgagca tccctcggcc gtccgcggag cggtcctttc cgatggccca
     3181 cccgttggag ccgatcgcgg cggcgtcgac gaccggtccg gtggcgggtg agggggcggc
     3241 ggggtcctgg gtcgaggcca gacgaagggt ggtgtgggtc cccgggcctg aggcatcggg
     3301 ggacaccgaa gccgtctcgg tcggcggatg ggcggtgggg atgtagcggg ccagtcgggc
     3361 gctgctggcg gtcaacgcga tggagcgggc gtaggcgtac acgtcgaccg gctccagggg
     3421 cttcagccag gcggcgccgg agcaccagcc cttgacgccc ttggcgccga cggccttcag
     3481 gtgggcgttc cagcccgcgg tgtaggcggt gatctgctcg ttcagctcct ctgagacctc
     3541 ctcccagtcc ttgaccgccc gctcgtggat cccgatcgcg cgccaggcgg cgtccgagtc
     3601 gacgttggcg tcgtcctcgc cggcgccgag ccagcgggcc cgttcgcctc gcaccttgat
     3661 gacctggtcg gccaggtcgc atgcccggtc ctggccgctg gcccagcctt cgccgaacga
     3721 cgcgtcggcc agggtcttgc cggtgatgtg gggaacgcct ccggtcgtgc ggcggatcgt
     3781 ggccgcatac cggtcgcccg agccgaccgt ggtcggtgct gattcgtcgt cgtcggagct
     3841 gctgcacgca gccaggacgg cgacgaggga gacggccagg atggccactc ccgccttccg
     3901 gaaggtgccg atcggactgg ccgcgcgttc cgccacttga cgctcccgtt ggtcgtcggt
     3961 ccgacgaacc gtagtgctcg ctacgagcgt ccgtgcctga acacggctca tgggtcgccg
     4021 accgaccggc cgacgagatg ggccagcggt ggtcgtgtca gcgacgtcgt tcgggagcgg
     4081 ttcagcggag gagcagccct cggggtcggg ccgcggcccg gtcgtcgaag caggcgatga
     4141 cgcgcacctc gtcggggtcg atctcgaaga tcgcctcctg gcggaaccgt cgggcgatgt
     4201 cgaggaccgt gtcgcggtcg gtgtcccaga ccaccaggcc gacctcggag tgctcggtgt
     4261 cgaccgaggt gcccaccgag cgatggtggc gggcgccggc ggcgtcgacc gcggcgatga
     4321 gctcggggcg tgccgcctgg ttctcttcgt cggagcgcca cgtccccatc gggttgtccg
     4381 acgtgagcgc ccacacggtt cccggatacg gccaggcgtc ggcgtcggag atcggggtga
     4441 gcgtgccgtc gaccagctcg gcggccacga cggtgcgcag gtagtcgtcc cagttgccgg
     4501 aaccggcacc cccgacgtgt gtctcggcgg gggtgccgcc gaacaccgcg gccagcagcg
     4561 ggcccagctc gcggctgatg gtcagcaccc gttcgtgcgc ggcctgcagc tcggcggctt
     4621 cggtggttcc ggccatgagg tcgcactcga cgaggacctg gtcgttcatc cagaagcagc
     4681 gggcgaagcc gagccgggcg ttgtggtcgt tgagctcgga caggagctcg gcgctgtgct
     4741 cgacgccgtc gaccgcgacc gcgaacacct ggatcctcac cggttcgtcg tcgaccaggc
     4801 gggcgaacac cgggatgtcg tggctcagcc ggtagtggcc gtcggtgtcg cgctgcacca
     4861 cggtggggcc gaagacctcg gccagcacct gctcggtgga gaaccgcatg cgctcgaagg
     4921 tggtctcgat gggcaggatg agcgcgtcgc ggtcggcgct gctgacgcgg atggcgttgt
     4981 gggcgaagtc cggaacgacg acggtgatcg gaccctcgag tccgatcagg gcgtcggcca
     5041 gctggctggg tggcaccgcc gcggtgcgcg agccctccgc ccgggcgcgc agctggaact
     5101 cggtgagccc caggccgggc cagcggaggt cgatcatgat cccctcgccg ccgacgacgg
     5161 cgagccagaa cagcgggtcc ggctcctcgt cgtcgatgtg ctcgaccgcc ggacgacgcc
     5221 gggccacgtc gatgagggcc gccagctctt cggcgtcgac ctcggcccac gcgccgtcgg
     5281 cggccaactc ggacgcgaag acctcgtcga tgctgggata ggcccgaggg tccgcggcga
     5341 ccgtcagggc ctgcgtgtcg gtgcgggcgg tgacggcgag caccgagccg tcgtcgtcgg
     5401 tgaccaggtc gatggtggcc gcctcgccgg tgagcgcccg gccgaaggcg aactcgacca
     5461 gtcggggtga cagcaggatg tcgtaggtcc cgtcgtcggt gccgccgcgc tggcggacga
     5521 cccgtcgacc gtcgccggac caccagtacc gcctggcgcc gtcgcccacc atccgcagca
     5581 tcccgagtac ggcgaactca tcgggcgcgg cgaaggtcac caacggcccg acgcccaacc
     5641 actcgtcggc cggcacctcg atcgtcgtcc tggtccccac cgtccgctcc cctcggtcga
     5701 ctcctggtcc gtcgacagta ccgggcggca ccgacagttc gatcctggtc ccgacccgat
     5761 cccgacgatc ctgacgatcc cgccgggcgg cggtcgagtc tcgggatagc gttcgccggg
     5821 acgcaggagg cgcggtgatg ggtgaagctc cgaccggacc cgaccacggg ccggtcctcc
     5881 gctacggcga cggtcagcgc gcgctgcagg accgcttcga cacccgacgc ctggccgacc
     5941 gtctggccga gaccgccacc gacgacgtga ccggctaccg gtccttcatc gagtcgctcg
     6001 acaccttctt cctggcgacg gtcgacgagc acggccagcc gcagtgctcc cacaaggccg
     6061 gagacccggg ggtggtgcgg gtcatcgacg ggcacaccct ggcgttcccg agctacgacg
     6121 gcaacggcat gttcctcagc ctcggcaacc tggcggccaa cccggcggtc gggatgctgt
     6181 tcatcgactt cgagggcggc acccgcctgc gggtcaacgg gatcgcgtcg gtcgacctgg
     6241 acgacccgct gaccgccgag ttcccctgcg cccaggccgt cgttcggggc cgggtgaccg
     6301 cggcgttccc gaactgccgt cgctacgtcc acccccgccg gcgcgtcgaa cgctcgccgt
     6361 tcgtgccgac cggacaggac gacccgccgg tgccggactg gaagctcatc ccgtggttcg
     6421 acggccacct cgccgccgat gacccggcgc tcgacccgca gcgcccgagc gctccggcca
     6481 cgccggagtt ctgagcggac cgttcagaag caggcgtcgc cgggtccggc cacggtgccg
     6541 tcggggcagg tggagccggc gtagtcgtgc gggccggtga aggtcgcacc cgtccagtcg
     6601 gcggtggtga agtccacgct gtcgtagagg gccggctgcg cctgggcctg cgacacgaaa
     6661 ctgctcaacg tggcgccggt gaaccgggcg ttgcggaact ccgggtaggt ggccgacgcg
     6721 ctggtgagat ccgcgtcctg gaaggtcacc gacggcttgc cgtcgccggt gtagttgacc
     6781 cacgccgacc ggtaccagcc gttgggggcg gtacccgccg ggtcctcgct cagcagggcc
     6841 gggtcgccga actgcgcacc gatcaggctc gaccgctcga acatcacgat ggcgctgaag
     6901 cggctgggca ccgcgttcat gatgtcggtg caggttgacc ccgtctcgcc ccggaacttc
     6961 gcgccgtcca ggtcggagtc cgacaggttc accgagcaca tgtgcaggtc gacgaacgtg
     7021 gcgccgaccg cggtcgtgtt cggcagcgtg atgtcgatga gctccccgcc gacgaaggtc
     7081 gctccgtcga agcggccgcc gctgccgtcc tggcaaccca cgctcacgcc gtggatcacc
     7141 gcgttggtga agttcgcctt gctcagcgac agaccccgga acgcgaactg ggttcccccg
     7201 agcaccgccc cggtgaagtc ggtcgccgcg tcggtcacga cgctgcgctg gccgttggcg
     7261 gcgagcgtgt cgttgtcctg gtgcaggtag aacgacggca cgccgagatc cgcgccgtag
     7321 gtctcgccgc agacgtccac gccggtgtcg acggtcgcct cgttgtagcc ggcgcagtac
     7381 ggcccggtgc acgccccgac gggtgtgccc accgcgccgc caccgccggt ggatcctccg
     7441 gccggaggtg tgaacccacc gaagtcgaac accgcgccga gcaggttcgc gcccaggaac
     7501 agcgctccca gcaggatcgc ccccgacaga atcgccccgg ccatgtcggc cccggtcagg
     7561 ttggccccgg tcaggttggc gcccgacagg ttggcgttgg tgaggttggc gcctgacaga
     7621 tcggcgccgg tcaggtccga cccccggagg tcggctccgg agaggtccac gccgctgaga
     7681 tcgagcccga tgagcgagca gttgtagagg ctcgccccgg ggccgatgac cggcgggctc
     7741 atgcaaccgg agccggtggg agccatccca ccgccggtcg ggttgttggt gcacgaaccg
     7801 gcgacgagcg cgacggcgac gcacgcgacg acagcgatgg ccttccctct ccacagcatg
     7861 gggtggaagg tacagcatcg agggacgtcg gtgcatcgga tccattccgg tcggcagcgg
     7921 ccgcgtgagg ctgccgccga ccgtgagtgg tcactccggt gcgtggcaga ccgcttcgac
     7981 gttgttgccg tcggggtcgc ggacgaaggc gccgaagtag cccgggtggt actcggggaa
     8041 ctcccgcggt tcgtgcagga cctcggcgcc gagcgcgacc gcggcgtcga agaaggcgcg
     8101 gaccgcggcc cggtccgggg cctggaaggc gatgtggtcc tcacgccagc cggtgtggct
     8161 gtccgacagc gggccgagcc agaagtcggg ctgggtggtg ccgtagccga tgacgccggg
     8221 gtggggctcc atcagacggg aggcaccgag cggtgccagg accctgtcgt agaacgccgc
     8281 gctggcggcg acgtcgctgc actggattcc gagatgatcg atcatggcgc catcgtgcca
     8341 gggggctgtg acgctccgtc cgcgaggagc tcgcggaccc gggggatcac ctcggcgccg
     8401 aacagctcga tgctgtggcg ctgtagggcg tcgggcatcg gacccgaccc gatcttgagc
     8461 gtgaaccggc tcagtccaag gacccgggag gtggccgcga tcttgcgggc gaccgtctcg
     8521 ggtgagccga cgtagagcgc gcctctcgga ccgacctggg cctcgaagtc cgatcgggtc
     8581 atcgggctcc agccgcgttc ggcggcgatg cgcccgtgca ggacccggtg gtgaggccag
     8641 gcctcctcga tcgcctgctc gtcggtgtcg gcgacgtggc cgggggagtg gcacccgacc
     8701 gggaggctgt cgtggccgaa ctccccgagg gcccgatggt agaggtcgac gaacggggcg
     8761 aagcgggcgg gctcgccacc gatgatggcg agcatcagcg gcatccggtg ccgggcggcc
     8821 cgcaccaccg actcggggct cccaccgacg ccgacccagg tgcggatcct gcccgactcg
     8881 gtccgggggt ggacgacctg gtcgcgcaac ggcggccggg tccggcccga ccaggtgacc
     8941 ggttcctcgg tgagcagctc ggcgagcagc gacagcttgt cgtcgaagag cacctcgtag
     9001 tcgcgcaggt cgaatccgaa cagcgggaac gactcggtga acgacccgcg tccgacgatg
     9061 atctcggccc ggccgttcga cacggcgtcg agggtggcga aacgctcgta gacccggatc
     9121 gggtcgtcgg agctgagcac ggtgaccgcg gtcgacagcc ggatccgctc ggtgcgcgcc
     9181 gcgatcgccg ccagcacgat ctcgggcgcg gacagggcga agtcgtcgcg gtggtgctcg
     9241 ccgaggccga tcacgtccac cccgacccgc tcggcgagca cggcctggtc gaccacgtcg
     9301 cggatgaccc gggccggatg ctgcggcacg ccgtccggat cgacggtcat gtcgccgaag
     9361 gtgtcgatgc cgagctcgat ggtcgtcggg ggcacccggc gacgttagcg accggtcctc
     9421 ggggacggag cggccgtgct tgacaagtcc ataggagtcc tatcaaactg ggtgggcccc
     9481 aggaggggcg accgcctcgg accgaaagga cctgccatga ccgctcccgc tggctacacc
     9541 tacgacgacc cgtcgctgag cccctcaccg gtcaccctcg ccgacctcga cgacctgctc
     9601 gcctcggtgc tgtggtccga agccgacgct gccgcgctcc ggcgggccgg cgagatcctc
     9661 gtcccgcaga ccgaggcgat cctcgacgtg tggtacggct tcgtgggctc gaacccccat
     9721 ctggtggcca ccttcgccgg ctctgacggg acgcccgacg gcgactacct ggctgccgtg
     9781 cgcgggcgct tcgggcggtg gatcgaggac ctgtgccgac gcgacttcga tgccggctgg
     9841 ctcgcctacc aggacgagat cgcccgtcga caccacacca gcggcaagaa ccggacggac
     9901 tcggtcgatt caccttcggg tcacgtcccg ctgcgccacc tgttcgcgct ggtcgttccg
     9961 atcaccgtca ccatccgtga cttcctcgcc tcgggtgcca ccgacgaggt cgagctggac
    10021 gccatgtacc aggcgtggtt caaggccgtc acgctctccg cgactctctg ggcgaggcct
    10081 tacagtcctg atctgtggtg agctcagcgt cggtggcggg gggtggagaa ccccgctcgg
    10141 aggacgatcc gaccgtgccg gagagggtgg tggcggcgtt ggaccgcatc gcccggggcg
    10201 tgcgcgccca ccggcagacc gtcgccaccg acgcgggggt gacggtgctg caggcggacc
    10261 tcctgcgtgc gctccaccac ggccccccgc cgccggcgct ggccggtccg ctcgccgcgg
    10321 acctgggcgt gagccagccg acggtctcgg actccctggc ggcgctcgag cgcaagggtc
    10381 tggtcgagcg gcggtccacg ccgggtgacc gccggcgcag cacgttcgcg ctcacacccg
    10441 acggcgaccg gctggccggt gggctccgac gggccgacga cgtgctcgcc gacaccgtcg
    10501 ccgcgctcga ccccgacgac caggagcacg tgctgcgcgg gctcctcgac atgatcaccg
    10561 gcatgttgga caaccggatc ctggccgtgg cccgcacgtg tccgacctgt cggtacttcg
    10621 aggcggggac cgacgggccc agatgctcgc tgctccaggt cccgctcgca cccggggacc
    10681 tgcgggtgga ctgcccggac cacgagccgg tctgagccgg accgacgcga ccgctgccgc
    10741 tccggcgcgg tcgagccagg gtcagagctg cagggcggcc ttgccggccg cgacgagcac
    10801 cgggtcccac accggagaga acggcggagc gtaggcgagg tccatccccg cgacgtcgcg
    10861 cacgtccatc cggttccaca gcgcggtggc gaccacgtcg atgcgcttgg cggatccgct
    10921 cccgccgacg atctggccgc cgagcagccg accggtgccg cgctcgacga cgagcttcac
    10981 ggtgatcggg tccgcgccgg ggtagtagtg cgccctcacc gcggcgcggg cggtcacggc
    11041 gaaagcgtcc agcccggctg cggccgcctc gcgctcgctg aggccggtcc ggccgacctc
    11101 ggtcgaaccc accttggtga tggcggtgcc gaggaccccg ccgaaggtcg aaggggtccc
    11161 gccgatgttg gagccgacga cgcgaccctg cttgttggca tgggtcccga gagcgatcac
    11221 gacccgttct ccggtgatgc ggtggtgtga ctcgacgcag tctccggcgg cccagacgcc
    11281 atcgacctgg gtgtgcaggc ggtcgtcgac gacgatcccg cccgaggctc cgaccggtat
    11341 tccggccgcc gcggccaacg ccgagttggc ccgtactccc aggccgacga ccgacagatc
    11401 ggcggggatc tctccggcgg tggtatcgac cgcggtgagg cgtccgccct cgtggacgaa
    11461 gccggtgacg ccggtgccga gcaggacctt cacgcccagc tcctcgagcg cggtggtgac
    11521 caggcccgcc atgtcggcgt cgagggtcag catcggctgg tcggccagct cgaccagcgt
    11581 cacctcgagg ccccgcgcgg cgaacgcctc ggcgacctcg acgccgatgt agccgccgcc
    11641 gatcacgacc gcgcgccgcg ccccggcgtc gatggcggca cgcaccgcgt cggcgccggg
    11701 gatggtccgc aggtcgaaca cgcctggccc gtcgatgccg ggcagcggcg gcttgacggg
    11761 ggaggcgccg gtggcgatga ggagctctcc gtacggttcg atggtggtcc cgcctccgtc
    11821 gagcgggcgg acctcgacgg tgcgggccgc cgtgtcgacg gccgtgacct cgtggcgggt
    11881 gcggacgtcg atgccccggg tgcggaactc ctcgggcgtg cgggcgacga gtcggtcccg
    11941 gtcggtcacc tcgccgccga cgaagtaggg gagcccgcat gccgcatagg aggtgaactc
    12001 gccgcgctcg aacacgacga tctccagatc gggtcgcagc cgccgggcct gggccgccgc
    12061 gctcatccct ccggcgtcac cgccgatcac caccagccgg tcagtcatcg ttcacctccg
    12121 gggcgacctg cccgctcgaa gcatacccct aggggtacag gggtgcccgt tccgcctccc
    12181 ccgggggtag accggcggtg gaggtacgag atgagactga acagctacga ggtcgacccc
    12241 ggtgggcaca agcggctgat cggctgggcc gagcacctgg atcgagggat cctgcccaag
    12301 gagctccgag cgctggtcga gacccgggtt tcgcagatca acgggtgcgc gttctgcctt
    12361 gccatgcaca ccgacgaggg tcggaccgcc ggtctgcccc aggccaagct cgacacggtc
    12421 gcggcgtggc gtgacgaccc gacgttcacc gaccgggaac gggccgcact cgacctggcc
    12481 gagacgatga cccgtatcgc cgacggcggc cgggtgtccg acgacgtgtg ggacgcggcc
    12541 gcagcggcct tcgacgaggc ggagctcgcc tcgctggtcc aggtggtcgc gatcatcaac
    12601 gcgttcaacc gcatcaacgt ggccacggag cggggctcga accactacct ggagtacgcc
    12661 tctcatcgct gaccggcgcc ccggtcccgg ggcaacaccc cgcgccagcg gggtcgggcc
    12721 cacacccggt cagcgtgcag ggatcgcacc actggcggcg aagacctgcc aggacggctg
    12781 gtcgtcgccg ccgatcagct cgaccactga gccgtcctcc acacgggaca cgagctggac
    12841 gacgagccgg tcgggagggc tggtcggccc ggtggccacg gcgagcacac cgtgctgcgc
    12901 gctccacagc accagcggct cgccccgcaa ccggcacagg tcaggacggt ccagcgtggc
    12961 ggccaaccga acggagtcgg ccacgctcaa cgttgaggtg tcaccgtcga ggaacctcac
    13021 gttggcgacc ccgtcggcag ccgtgtcggt gacggtggcg ggccgcgccc ggggactggc
    13081 gtcgggcccg agcgctgccc ggacccgggt gtggtggagg gccgaaaggg gggacatggc
    13141 ttctcctcat cgtcgccggc gggggcaccg tccctggtcg ggggttgcca ggccgtcgtc
    13201 gagccgggtc tctcgggcct tctggatgag ctgaccgaca ccgtaccgag ggggtctgac
    13261 agtcgaccga gcgccgtcac cccgccctct tgtccagccc ccgccccacc gaacttgtag
    13321 cccacgcgtc accaccgcga ccgcgggcta caagttcgca gggcgtcgcc gaacttgtag
    13381 cccacgcgtc accaccgcga cagcgggcta caagttcgca gggcgtcgcc gatggtcgag
    13441 gcagctcacg gctccggccg gagcattggg ccctcgggct gttgagcgtg ttgcccggcg
    13501 gcgcgtcgga gactgagatc gggcgatcgt cagccgtcgg tggagccgtc cacggggtcg
    13561 aaagcggcgt cgagctcggt gagcgctcgg cgcacgccgt cgggatcgac caggcgctgt
    13621 cgggcgacct cgagcgccag agccggaggg aggcagtcgg cgaacttcat gttggccgcg
    13681 a
//
LOCUS       DAOQOD010000079        13577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1605_length_13577_cov_10.080461, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000079 DAOQOD010000000
VERSION     DAOQOD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_13577_cov_10.080461"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLZ72_09095"
     CDS             <1..443
                     /locus_tag="PLZ72_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HNA36490.1"
                     /translation="VPSGSVLVWDPIGGPIGVGVAELLAAAGHQVHIATPDQIIGNEL
                     ARAGDLGPANARLAQAGVELHRRSILRVVDGDGALLEDRFSSEVRRLDVASVLDAGHR
                     LPDDELWIRVAALFGAGAVAAGDCVAPRTVHEAVLEGRRAVLGG"
     gene            591..944
                     /locus_tag="PLZ72_09100"
     CDS             591..944
                     /locus_tag="PLZ72_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36491.1"
                     /translation="MIETTRTALENTISQAIERAKAANSAFRTHDDVAVALPGGLFGH
                     VRNGRLISFGLEPVGDVYVDQQDHVSDLPDGGPGHLLWGRIAAVAGGATAAASAVAGV
                     VTLIVGRRHPSAPSS"
     gene            1042..3054
                     /locus_tag="PLZ72_09105"
     CDS             1042..3054
                     /locus_tag="PLZ72_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="HNA36492.1"
                     /translation="MAEQLLWTPLQLGPVTVRNRIVFSAHLTNYAQDGKPTEQHAAYY
                     QERAAGGVGLIITEEHSTHPTDWPYEKLIHGFHRDVIPGYRRITDAVHRHRTPIFAQI
                     NHNGGQASSLYSRLPVWAPSPVADPLFREVPKAVDRHEIAEIVAGYARVAENCAEGGF
                     DGIELQCSHSSIVRGFLSPATNRRTDDYGGPLANRARILLEIVAAVRAAIGTELALGV
                     RICGDELIEGGTTIDDAVEVARMVEGSGHVDYINTSIGVATASLFMIEASMHIPPGYA
                     MFIPSALRQAVDLPVVGVGRFKDPLQAERALAEGQCDLVGVVRGQIADAEFVAKARAG
                     FTDDTRLCLSCNQECVGRMGLNRWLGCIENPRTGRESEGVGEARPVAVGRRVLVIGAG
                     PAGLQAAIAAARNGHHVTVLEREPHAGGQVRLAATVPNRAELGDLVRNQVHECGRLGV
                     TIEYGVDATASEVERRRPDSVIVATGAVAARPWWAPPEADWIVDVRDVLTGAAQPTGD
                     VVVLDELGFHHATSVAEVLADRGCRVEVITPGMVVGQDLGITLDLETWWIRAGAKGIV
                     QSTELVPMGVEDVVDGGHVVNLQHHPTGSMHTRTPDWLVLAVPADPADGLYHELRSSA
                     ELRDRGVDVVRIGDAVAPRRAHAAVIDGERVGAAVPSAAPAAATAS"
     gene            3139..4731
                     /locus_tag="PLZ72_09110"
     CDS             3139..4731
                     /locus_tag="PLZ72_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36493.1"
                     /translation="MATESDHPSRGLAVAGSWWLLTMVVLGVYLVSPVRDATDADPAF
                     APLSAHSLVHHGDLTLDRFGARRLIGHPVVVSDGSLDAGFVVSGVGSTGWEQLSAAVA
                     DPDVDVVDYFPWTGAVLAVPAVLVADGWAALTGTADSGELLAGGGFAWLHAASASLVV
                     LGAVLAVRATAIAALTGPMGRRRVLSNATALVVALGTSAYSTASRALWQHTPSLLLLT
                     LALWCAVRIDRNVTGPGPVDPTGSDPEPTPEPRDPVRLQGWTVGLAAASAAAAVARPT
                     NLAFAVLIVAWVLYRNRHRPLATAASALLGGSAVVCAFVGLSLLLVGRVVPQYYSAGR
                     LQLGGWFAEAVAANWVSPSRGLLWASPIVVMSVAGTVVALRSAGRGEPVVPGRALVVA
                     LWATVAAVTVAVSAFSQWWAGHAFGPRFMTEAVPALVVLALPAVDRLAGGAAADRDAR
                     HPGRRTAALVAVCALALWSVAFHAVGSVAGASGCWNAYPIDVDSDPARVWSLPDAQVL
                     EPVHRVLDPQRRAAQDAVCVGR"
     gene            complement(4735..5502)
                     /locus_tag="PLZ72_09115"
     CDS             complement(4735..5502)
                     /locus_tag="PLZ72_09115"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HNA36494.1"
                     /translation="MSSTPQGWFPDPAGRHQLRWWNGRAWAAAVADDGVQSFDPDGLS
                     PGAPDQPRVDASDAEHAEVVRACHVGALPGDDALTAPVLSVWHRRSGLVDLHPTWSVH
                     DGVGRWLGTFSLAVSGPTPDDRSFVLHRPDGTSLSVTPDRPRAVARLIDSDGTEVARV
                     APVGHSAQRVEVVVDGMVVTSTVYSVGEVALADNSGATIARLVPPPLGVRRRIGRRVG
                     WDPVLVSEQTSVADPVTGRGVNALALAWEHTVREWAG"
     gene            complement(5499..6317)
                     /locus_tag="PLZ72_09120"
     CDS             complement(5499..6317)
                     /locus_tag="PLZ72_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36495.1"
                     /translation="MTTYVLIHGGGSTARFWDRLAPLLEVDGDRVLAVDLPGRGARPA
                     DLAAITVDDEVASVVADVSATAPDGPVVVVAHSSGGLVVPGVVAALCDDEGGSRVTAV
                     VLVAALVPPEGGTGLDCMKPHHAEGLRSAVAALGPDDEPVTLPGAPADPEPFRTATGG
                     DPLDDDTLVFVVEPVRLVPDTVHHYFQPVRWSQVPPSVPLTYVLTLRDRPVPSELQQR
                     MAHRLPATSTVDVVEVDTGHLAPITHPELLAGLIRSARRRGAERDDPEQRGAPR"
     gene            complement(6369..7298)
                     /locus_tag="PLZ72_09125"
     CDS             complement(6369..7298)
                     /locus_tag="PLZ72_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNA36496.1"
                     /translation="MGIDHDHSHGGGGHNHGSALARSGEAAKRPLTLALGLSLLTFVV
                     QVVVGALTGSLALLTDSAHVLTDGVGIALALAAVILATRSRRPERTFGLYRLEVLSAL
                     ANAVLLLAVAAWALYEAVQRIQDPPEVPGWPVLVVGVVGLITNLAALRLLSEGGSLAA
                     EGARLEVLADTVGSVGVVAGGAIIVATGATVIDAWVGLAIALWIVPRSLRLAASALRV
                     LVQAAPAGLDLDAIHDDLATIPDVVGIHDLHLWTLTSDMEVFTAHVMVSTDADHHSVL
                     DQARVLLADRHGIHHATLQVEPEDHQGCDELDW"
     gene            7356..8324
                     /locus_tag="PLZ72_09130"
     CDS             7356..8324
                     /locus_tag="PLZ72_09130"
                     /inference="COORDINATES: protein motif:HMM:NF038209.1"
                     /note="Built with help from friend_finder, bracket5, and
                     grep mycofactocin; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-associated electron transfer
                     flavoprotein alpha subunit"
                     /protein_id="HNA36497.1"
                     /translation="MAPTAPAEPSGRVAVVPVRAGTLPAGALDAARAAAGHVVVVGDG
                     AAAAAAGLAGVATEVRAWDTPGFAPAAWAATLAPVLAAERIVVLPASPDGRDLAPRVA
                     LELDRPVLAGAVSVHDDVVATACIGGSVLAEHLVADPVVVTVQPGPGSTGHGAAPRVE
                     TIEPTPVDGAGPDAVVVEVLPPDVTTMDLAEARRILGGGAGLDSAERFDRLTEVATAL
                     GASMGATRVVTDRGWVPHSRQIGTTGVVVDPDLYLAFGISGAVQHTAGLGDPAHVISV
                     NTDPHCPMMAMADLAVVADANATVDALADLLVGDPSEGDLPEGDGG"
     gene            8317..9627
                     /locus_tag="PLZ72_09135"
     CDS             8317..9627
                     /locus_tag="PLZ72_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA36498.1"
                     /translation="MADFDAIVVGAGPAGSAAAIELARSGASVCLIERGPYPGSKNMY
                     GGVIYGRILDEIIPEWWTEAPVQRWVTRRATMILTEDRALTVDYRSGAWGEPPYNGAT
                     AYRPDFDAWLAEHAVAAGATLVTSTTVTGLLRDGDRADGAIVGVRTDRPDGDLTARVV
                     IACDGVNSFVAKEAGLYGEVDAANYTLGVKETIALPREVIDERFGVRGDHGVDYEIMG
                     CTGDVPGGGFIYTNADTLAVGLVLSLPALARGGVRPEELLRRMKAHPAIAPLVEGGEV
                     TEYSAHVIPEAGFDMMPSMIADGLLVAGDAAAMCLAAGIWLEGVNFAIGSGSAAGKAA
                     ARAIRRADTTAAGLDSYRTRIEASFVMKDHRKLRQAPHLVMGELAQQKLPAMACGVAE
                     AMFHVDNPHPKPGLRRIVARQMKANGVRWRDAVSEGFRAWRTFG"
     gene            9641..9940
                     /locus_tag="PLZ72_09140"
     CDS             9641..9940
                     /locus_tag="PLZ72_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNA36499.1"
                     /translation="MSRPYPEISFEHRMSTVEFRVDEDRAHITVDDTKCRDCSVRGCI
                     TACPADLFVPTADGGILFNYEQCFECGTCYLVCNEEGAITWTYPEGGHGVVFHRT"
     gene            9937..10851
                     /locus_tag="PLZ72_09145"
     CDS             9937..10851
                     /locus_tag="PLZ72_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-associated electron transfer
                     flavoprotein beta subunit"
                     /protein_id="HNA36500.1"
                     /translation="MSTPGTPVPALVAACVKWVDLRPEVDRVHGTVTPSGHGGGFSAA
                     DRSAVEVALRLGEAWDARTVVVCAGPPAADAGLRDLLAAGADRAVRVDLPGADAADVG
                     AQTGEGAAALLAPVLSELGASVVVCGDVSADLGSGTVPAYLAHHLGAAQALGLLDVTV
                     SGSGHLRAVRRLDGGRREVLEVAAPAVLSVEGGVAELRRAPLSATLVAGDAPVEVRPG
                     RERHVGDGPRLRPWRPPTRVVEPPAGEHALDRIVSLTGALVDRTPPRTVELDPAAAAA
                     AIVEQLRAWGHLGADRDPDPGNGAAAGG"
     gene            10856..11563
                     /gene="mftE"
                     /locus_tag="PLZ72_09150"
     CDS             10856..11563
                     /gene="mftE"
                     /locus_tag="PLZ72_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HNA36501.1"
                     /translation="MRLAELSWPDVPEPSVLVVPLGSCEQHGPHLPLDTDTRIAEALA
                     AALVDRLGDGMVLGPTVGIGASGEHESFPGTLSIGTVVLEGVLVELVRSALPPPGSDR
                     PRPFRGVVVVNGHGGNVEATTRAAALLTDEGRDVLVWHPRVPGGDSHAGATETSMLLH
                     LCPEVVDLTRAEVGSTARWRDIGDAVRTGGLAAVSANGVLGDPTGATEEEGRRIVGML
                     VEQLCAAVDRWRDTLER"
     gene            11560..12393
                     /locus_tag="PLZ72_09155"
     CDS             11560..12393
                     /locus_tag="PLZ72_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HNA36502.1"
                     /translation="MSVDQSPPPARVAGPSTGRGTALVTGAARGIGAAVAARLAAEGY
                     RVVLADRCSGPDSDVVLGYRLASRDELDATAAAIGPAATGIELDVRDLDAVRRVVSTV
                     EDLRVVVTAAGVVWGGAPVWDMPGAAWQEVFDVNVTGTYGVIAAAVPRLLDQPEPRTG
                     RIVALASAGASRGLPHMAAYSAAKHAVVGLVRSVAGELADRGVTINAVAPGSTRTPIL
                     RASAEAYRLGAVEEFIPHQPIGRLLDPSEIADAVAWLCSPGAAAVTGSVVAVDGGMTA
                     V"
     gene            12395..>13577
                     /gene="mftF"
                     /locus_tag="PLZ72_09160"
     CDS             12395..>13577
                     /gene="mftF"
                     /locus_tag="PLZ72_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HNA36503.1"
                     /translation="MRFRADRSLRILDRAGERVPVLGGSPLRLWRLTGAGGELVARLA
                     AGDDVVVAAGSPTAALVDRLLDAGALHPDPTSLPSRCSPSDVTVVVPVRDRPDALDGL
                     LASLGPFHAAGAGLVVVDDGSVDGPSHERVSQRHGATYIRRERSGGPGAARDDGIATA
                     TTPLVAVVDSDCVVPPGAGHWWEPLLRLFDDDRVAAVAPRVRTPPGPALLACYEGLRS
                     PLDMGADPARVSPGTRVSYVPAAALVLRTDAHRAVGGFDPDLSVGEDVDLIWRLADAG
                     HRIRYEPTAVLDHPPRRGLGAWMRQRVGYGSSAGPLDARHPWRVAPVRCSPWSAAGWA
                     AIVLGPGSAGPIAGAAILGISAALLPRRLADVRTGAAVRLAVSGHGAAGVQLARAAVR
                     VW"
BASE COUNT         1638 a         5002 c         5086 g         1851 t
ORIGIN      
        1 cggtgccatc ggggtcggtg ctggtgtggg acccgatcgg cggaccgatc ggcgtcgggg
       61 tcgccgagct cctggccgcc gccggccacc aggtccacat cgccacgccg gaccagatca
      121 tcggcaacga gctcgcccgc gccggcgacc tcggcccggc caacgcacga ctggcccagg
      181 cgggcgtcga gctgcaccgc aggtcgatcc tccgggtggt cgacggcgac ggggcgctgc
      241 tcgaggaccg gttcagttcg gaggtgcggc ggttggacgt ggcgtcggtc ctcgacgccg
      301 ggcaccgcct ccccgatgac gagctgtgga tccgggtcgc tgcgctgttc ggtgccgggg
      361 ccgtggccgc cggtgactgc gtggccccgc gcaccgttca cgaagcggtc ctcgagggtc
      421 gccgggccgt cctgggcggc tgaggaccca cccccggaag tgggtggcca ggggctttct
      481 gcccgttgcg ctccctcgtt cgagcggtac cctgagggca gcggatgccg ctgggggaac
      541 ggctggtacc agcgtcggtg ccgacggccg cgcgacaggg agaaccggac atgatcgaga
      601 ccaccaggac agccctcgag aacacgatca gccaggccat cgagcgggcc aaggccgcca
      661 acagcgcgtt ccggacccac gacgacgtgg cggtcgcgct gcccggaggg ctgttcggac
      721 acgtccgcaa cgggcgcctg atcagcttcg gcctggaacc ggtcggcgac gtgtacgtcg
      781 accagcagga ccacgtgagc gacctgcccg acggcggtcc cggccacctg ctgtggggtc
      841 gcatcgctgc ggtcgccggc ggtgcgaccg cagcggcatc ggccgtggcc ggcgtggtga
      901 ccctgatcgt gggccgtcgt cacccgtcgg cgccctcgtc ctgacgccct cgtcctgacg
      961 ctctcgtccg gacctgctgg cccgacccgc ccggtcctgg tcggttcgcg tcgcgtcggg
     1021 cgcggtgcct agcctgcggc catggcggag cagctcctct ggacgcccct tcagctcggg
     1081 ccggtcacgg tccgcaaccg catcgtcttc tccgcgcacc tgacgaacta cgcgcaggac
     1141 ggcaagccga ccgagcagca cgcggcctac taccaggagc gtgccgcggg cggcgtcggg
     1201 ctgatcatca ccgaggagca ctcgacccac cccaccgact ggccctacga gaagctgatc
     1261 cacggcttcc accgtgacgt catcccgggc taccgccgca tcaccgacgc ggtgcaccgg
     1321 caccgcaccc cgatcttcgc ccagatcaac cacaacggcg gccaggccag ctcgctctac
     1381 tcccgtctgc cggtgtgggc gccgtcaccg gtggccgacc cgctgttccg cgaggtgccc
     1441 aaggccgtcg accgccacga gatcgccgag atcgtcgccg gctacgcccg cgtggcggag
     1501 aactgcgccg agggcggctt cgacggcatc gagctccagt gctcgcacag ctcgatcgtc
     1561 cgggggttcc tgtcgccggc gaccaaccgt cgcaccgacg actacggcgg tccgctggcg
     1621 aaccgggccc ggatcctcct cgagatcgtc gccgcggtgc gcgccgcgat cgggaccgag
     1681 ctggcgctcg gggttcgcat ctgcggcgac gagctcatcg agggcggcac cacgatcgac
     1741 gacgcggtcg aggtggcccg gatggtcgag ggaagcgggc acgtcgacta catcaacacc
     1801 tcgatcggcg tggccaccgc ctcgctgttc atgatcgagg cgtcgatgca catcccgccc
     1861 ggatacgcca tgttcatccc gagcgcactt cggcaggcgg tcgacctgcc ggtcgtcggc
     1921 gtcggacggt tcaaggaccc gctccaggcg gagcgggcgc tcgccgaagg gcagtgcgac
     1981 ctggtcggcg tggtgcgcgg ccagatcgcg gacgccgagt tcgtggccaa ggcccgggcc
     2041 gggttcaccg acgacacccg gctgtgtctg agctgcaacc aggagtgcgt cgggcggatg
     2101 ggtctcaacc gctggttggg ctgcatcgag aacccccgga ccgggcgcga gtccgaaggg
     2161 gtcggcgagg cgcggccggt cgcggtcgga cgacgggtgc tcgtgatcgg ggccggaccg
     2221 gccgggctgc aggccgcgat cgcggccgct cgcaacggcc accacgtgac cgtgctcgaa
     2281 cgcgagcccc acgccggcgg ccaggtccgc ctggcggcca cggtgccgaa ccgggccgag
     2341 ctcggggatc tggtccgcaa ccaggtccac gagtgcggcc gcctgggagt gacgatcgag
     2401 tacggcgtcg acgcgaccgc gtccgaggtc gagcgccgcc gccccgactc ggtgatcgtg
     2461 gccaccggtg cggtggcggc gcgcccgtgg tgggcgccac cggaggccga ctggatcgtc
     2521 gacgtccgcg acgtcctcac cggggccgct cagcccaccg gcgacgtcgt cgtcctcgac
     2581 gagctgggct tccaccacgc cacgtcggtg gccgaggtgc tcgcggatcg gggttgtcgg
     2641 gtcgaggtca tcaccccggg catggtcgtc ggccaggacc tgggcatcac gctggacctg
     2701 gagacctggt ggatccgcgc cggggccaag gggatcgtcc agtccaccga gctggtcccg
     2761 atgggcgtcg aggacgtcgt agacggcggt cacgtcgtca acctgcagca ccaccccacc
     2821 gggtcaatgc acacccgcac ccccgactgg ctggtgctgg cggtgccggc cgacccggcc
     2881 gacgggctgt accacgagct gcggtcgtcg gccgagctgc gcgaccgcgg ggtcgacgtc
     2941 gtgcggatcg gcgacgccgt ggccccccgc cgcgcccacg ccgcggtcat cgacggcgag
     3001 cgcgtcggtg ccgcggtccc ctcagcggca cccgcggcgg ccaccgcctc ctgatcggag
     3061 cggtgcacgg ccgctgcggg agcggtccgc cccgggtggc tcaccggatg ggccccgacg
     3121 ttgtgtgaga ctcccgcggt ggccaccgag tccgaccacc cgtcgcgcgg gctcgccgtc
     3181 gcgggatcgt ggtggctgct caccatggtc gtcctcggcg tgtacctggt cagcccggtc
     3241 cgtgacgcca ccgacgccga tccggccttc gcccccctga gcgcccactc gctggtgcac
     3301 cacggtgacc tgaccctcga ccgattcggg gcccgccgcc tcatcggcca tcccgtcgtg
     3361 gtgagcgacg gctcgctcga cgcgggcttc gtggtgtccg gcgtcggctc gaccggttgg
     3421 gagcagctga gcgcagcggt cgcggacccc gacgtcgacg tcgtcgacta cttcccgtgg
     3481 accggtgccg tgctcgccgt tcccgcggtg ctggtcgcgg acgggtgggc ggcactcacc
     3541 gggaccgccg acagcggtga gctgctcgcc ggtggcggct tcgcctggct gcacgcggcg
     3601 agcgcatccc tggtggtcct cggtgcggtg ctcgcggtgc gggcgacggc gatcgcagca
     3661 ctgaccggcc cgatgggtcg ccgtcgggtg ctgtccaacg ccacggccct ggtggtcgcg
     3721 ctcggcacct cggcgtactc gaccgcgagc cgggcgctgt ggcagcacac cccgtcgctg
     3781 ctgctgctca ccctcgcgct gtggtgcgcg gtgcgcatcg atcggaacgt gaccggaccc
     3841 ggaccggtgg accccaccgg gtccgacccg gagccgacgc cggagccacg ggacccggtc
     3901 cggctgcagg gctggaccgt cggcctggcc gcggcgtcgg ccgcagcagc ggtggcccga
     3961 ccgaccaacc tggcgttcgc ggtgctcatc gtggcgtggg tgctgtaccg caaccggcat
     4021 cggcccctcg ccaccgcggc atcggccctg ctcggcggat cggcggtcgt ctgcgccttc
     4081 gtcggcctga gcctgctgct ggtcggccgg gtcgttccgc agtactactc cgcgggacgg
     4141 ctgcagctcg gcggctggtt cgccgaggcc gtggccgcca attgggtcag cccgtcacgg
     4201 gggctcctgt gggcgtcgcc gatcgtcgtg atgtcggtcg ccgggacggt cgtcgcgttg
     4261 cgctcagcgg gccgggggga gccggtggtc cccggtcggg ccctggtcgt ggcgctgtgg
     4321 gccacggtcg ccgcggtgac cgtggcggtc tccgcgttct cgcagtggtg ggccggtcac
     4381 gccttcggtc cccggttcat gaccgaggcc gtcccggcgc tggtggtcct ggccctcccg
     4441 gcggtcgacc ggctggccgg gggcgccgcg gccgatcggg acgcccgaca tccgggtcgt
     4501 cgcaccgctg cgctggtcgc ggtctgcgcc ctcgcgctgt ggtcggtggc gttccacgcc
     4561 gtcggttcgg tggccggcgc cagcgggtgc tggaacgcgt acccgatcga cgtcgactcg
     4621 gatccggccc gggtgtggtc gctccccgat gcccaggtgc tcgagccggt gcaccgggtc
     4681 ctggatccgc agcgccgcgc cgcgcaggac gcggtgtgcg tcgggcgctg acggtcagcc
     4741 agcccactcc cggacggtgt gctcccaggc cagcgccagg gcgttgaccc cacggccggt
     4801 caccgggtcg gcgaccgagg tctgctccga gaccaggacc ggatcccacc cgactcgacg
     4861 tccgatccgc cggcggacgc ccagcggtgg agggacgaga cgggcgatgg tggccccgga
     4921 gttgtcggcc aacgcgacct cgcccacgga gtacaccgtg gaggtcacga ccatgccgtc
     4981 gacgacgacc tcgacccgtt gggcgctgtg gccgacgggc gcgacgcggg cgacctcggt
     5041 cccgtcgctg tcgatcagcc gcgcgacggc ccgggggcgg tcgggggtga ccgacaggga
     5101 cgtgccgtcg ggtcggtgca gcacgaagct gcggtcgtcg ggtgtcgggc ccgagacggc
     5161 caacgagaac gtccccagcc atcgccccac accgtcgtgg accgaccagg tcgggtggag
     5221 gtcgaccagc ccgctgcgac ggtgccacac cgagagcacc ggagcggtga gcgcgtcgtc
     5281 accggggagg gccccgacgt ggcaggcgcg gacgacctcg gcgtgttctg cgtccgatgc
     5341 gtcgacgcgg ggctgatcgg gcgctccggg gctcaacccg tcggggtcga aggactgcac
     5401 gccgtcatcg gcgaccgctg cggcccaggc ccgaccgttc caccaccgga gctggtgacg
     5461 tccggccgga tcggggaacc acccctgcgg cgtgctgctc accggggtgc gcccctctgc
     5521 tcggggtcgt cacgctccgc cccacgacga cgcgcggagc ggatcagccc ggccagcagc
     5581 tcggggtggg tgatgggggc caggtgcccg gtgtcgacct cgacgacgtc gaccgtcgag
     5641 gtcgccggca gccggtgggc catccgttgc tggagctcgc tcgggacggg ccggtcgcgg
     5701 agggtgagga cgtaggtcag cggcaccgac ggcggcacct gcgaccagcg caccggctgg
     5761 aagtaatggt gcacggtgtc ggggaccagc cgcacgggct ccaccacgaa caccagcgtg
     5821 tcgtcgtcga gcggatcgcc gccggtggcg gtgcggaacg gctcggggtc cgcgggcgcg
     5881 ccgggcaggg tgaccggctc gtcgtcggga ccgagcgcgg cgacggcgct tcgcagtccc
     5941 tcggcgtggt gtggcttcat gcagtccagg ccggtcccgc cctcgggggg tacgagcgcg
     6001 gcgaccagca ccaccgccgt cacccgggac cctccctcgt cgtcgcacag ggcggccacc
     6061 acgccgggga cgacgagccc gcccgaggag tgggcgacga ccacgaccgg gccgtccggc
     6121 gccgtggccg agacgtcggc gaccaccgag gccacctcgt cgtcgacggt gatcgccgcc
     6181 aggtccgccg gacgtgcgcc ccggcccggg aggtcgacgg ccaggacccg gtccccgtcg
     6241 acctccaaca ggggagcgag acggtcccag aagcgggcgg tggagcctcc gccgtggatc
     6301 aggacgtagg tcgtcaccgc gctctgccta gcaggctcga cccggcatgc ggtgcgcgtc
     6361 agctgctcct accagtcgag ctcgtcgcag ccctggtggt cctcgggttc gacctggagt
     6421 gtggcgtggt ggatgccgtg ccggtcggcc agcaggacgc gggcctggtc gagcaccgag
     6481 tggtgatccg cgtcggtgct caccatcacg tgggcggtga acacctccat gtccgaggtc
     6541 aacgtccaca ggtgcaggtc gtggatgccg accacgtcgg ggatcgtggc caggtcgtcg
     6601 tggatcgcgt ccaggtcgag cccggcgggg gcggcctgga ccaggacccg cagcgcgctg
     6661 gcggccagcc gcagcgaccg gggaacgatc cacagggcga tcgccaggcc gacccacgcg
     6721 tcgatcaccg tcgctccggt ggcgacgatg atcgctccgc cggcgacgac gcccaccgag
     6781 ccgaccgtgt cggccagcac ctcgagacgg gcgccctcgg cggccagcga cccaccttcc
     6841 gagagcagcc ggagggcggc caggttggtg atcagtccga ccaccccgac gacgagcacc
     6901 ggccaccccg gcacctcggg tgggtcctgg atccgctgga ccgcctcgta cagcgcccac
     6961 gcggcgaccg cgaggagcag cacggcgttg gcgagcgcgg agaggacctc gagccggtac
     7021 agcccgaagg tgcgctccgg acggcgggac cgggtggcca ggatcaccgc ggccagagcc
     7081 agggcgatgc cgaccccgtc ggtcagcacg tgggccgagt cggtcaacag ggcgagcgat
     7141 ccggtcagcg ctccgacgac gacctggacc acgaaggtga gcaggctgag cccgagtgcg
     7201 agggtgagcg gtcgcttggc cgcctcgccg ctgcgggcca gcgccgagcc gtggttgtgg
     7261 ccgcccccgc cgtggctgtg gtcgtggtcg attcccatcg gttcccatta tgccaactcg
     7321 gaggggtcga cacctccggg ctaccttcct ggtccatggc cccgaccgcg cccgccgaac
     7381 cgagtggtcg ggtcgcggtc gtcccggtcc gggccggcac ccttcccgcg ggggccctcg
     7441 acgccgcccg cgccgcggcc ggtcacgtgg tcgtcgtcgg cgacggtgcc gcagccgcag
     7501 ccgccggcct ggccggcgtg gccaccgagg tccgggcctg ggacaccccc ggcttcgctc
     7561 cggcggcctg ggccgcgacc ctggccccgg tgctcgccgc cgagcggatc gtggtgctcc
     7621 cggcgtcgcc cgacggacgt gacctcgccc cccgggtggc gctcgagctc gaccgcccgg
     7681 tgcttgccgg ggcggtgtcg gtgcacgacg acgtggtcgc caccgcgtgc atcggagggt
     7741 cggtgctggc cgagcacctg gtggccgacc cggtcgtcgt cacggtgcag cccggtcccg
     7801 gctcgacggg ccacggggcg gcgccacggg tggagacgat cgagccgacc ccggtcgacg
     7861 gcgccggacc cgacgcggtc gtcgtcgagg tcctccctcc cgacgtgacc accatggacc
     7921 tggccgaagc gcgtcgaatc ctgggcggag gcgccgggct cgactccgcg gagcgcttcg
     7981 accgcctgac cgaggtggcg accgccctgg gcgcgtccat gggcgccacc cgggtggtga
     8041 ccgaccgggg ttgggtgccc cacagccgcc agatcggcac gaccggcgtg gtggtcgacc
     8101 ccgatctgta cctggcgttc ggcatctcgg gggcggtgca gcacaccgcc gggctcggcg
     8161 accccgcgca cgtcatcagc gtcaacaccg acccgcactg cccgatgatg gcgatggccg
     8221 acctggcggt ggtggccgat gccaacgcga cggtcgacgc gctcgctgac ctgctcgtcg
     8281 gcgacccctc cgagggcgac ctcccggagg gcgacggtgg ctgacttcga tgccatcgtc
     8341 gtcggcgccg gtccggcggg atcggccgcc gccatcgagc tcgcccgctc cggcgcgtcg
     8401 gtgtgcctga tcgaacgcgg cccctacccg ggctccaaga acatgtacgg cggcgtcatc
     8461 tacggacgca tcctcgacga gatcatcccc gagtggtgga ccgaggcccc cgtgcagcgc
     8521 tgggtgaccc gccgcgccac catgatcctc accgaggacc gggcgttgac cgtcgactac
     8581 cgcagcggcg cctggggcga gccgccctac aacggtgcca ccgcctaccg gcccgacttc
     8641 gacgcctggc tggcagagca cgccgtggcc gccggcgcca ccctggtcac gtcgaccacc
     8701 gtgaccggtc tgctgcgaga cggcgaccgg gccgacggcg cgatcgtcgg ggtgcggacc
     8761 gaccgtcccg acggcgacct gaccgcccgc gtggtcatcg cctgcgacgg ggtcaactcg
     8821 ttcgtcgcca aggaggccgg cctctacggc gaggtcgacg ccgccaacta caccctgggg
     8881 gtgaaggaga ccatcgctct gccccgagag gtgatcgacg agcggttcgg ggtccgggga
     8941 gaccacggcg tcgactacga gatcatgggc tgcaccggcg acgtccccgg cggcgggttc
     9001 atctacacca acgccgacac gctggcggtg ggcctggtcc tgtcgctgcc ggcgttggcc
     9061 cgcggcggcg tccgtcccga ggagttgctg cggcggatga aggcccatcc ggccatcgcg
     9121 ccgctggtcg agggcggcga ggtcaccgag tactccgccc acgtcatccc cgaggccggc
     9181 ttcgacatga tgccgtcgat gatcgccgac ggtctccttg tcgccggcga cgccgcggcc
     9241 atgtgcctgg ccgccggcat ctggctcgag ggtgtgaact tcgccatcgg atccggctcg
     9301 gcggccggca aggccgcagc gcgggcgatc cggcgcgccg acacgaccgc cgccgggctc
     9361 gactcgtacc ggacccgcat cgaggcgtcc ttcgtcatga aggaccatcg caagctccgc
     9421 caggctccgc acctggtcat gggcgagctg gcccagcaga agctgcccgc gatggcgtgc
     9481 ggggtggccg aagccatgtt ccacgtcgac aacccccacc ccaagccggg cctgcgccgc
     9541 atcgtggccc gccagatgaa ggcgaacggg gtccgctggc gcgacgcggt gtccgagggg
     9601 ttccgggcgt ggaggacctt cggatgaccg ggagcgcgtc atgagccgtc cctacccgga
     9661 gatcagcttc gagcaccgga tgtcgaccgt cgagttccgc gtcgacgagg accgggccca
     9721 catcaccgtc gacgacacca agtgccgcga ctgctcggtc cgcggctgca tcaccgcttg
     9781 cccggccgac ctgttcgtcc ccaccgcgga cggcggcatc ctgttcaact acgagcagtg
     9841 cttcgagtgc ggcacctgct acctcgtctg caacgaagag ggcgcgatca cctggaccta
     9901 ccccgaaggc ggccacggcg tcgtcttcca ccgcacgtga gcaccccggg caccccggtg
     9961 ccggcgctcg tcgctgcgtg cgtgaagtgg gtcgacctgc gccccgaggt ggaccgcgtg
    10021 cacggcaccg tcaccccctc cgggcacggc ggcgggttct ccgccgcgga ccgctccgcg
    10081 gtcgaggtcg ctctgcgtct cggtgaggcc tgggacgccc gaaccgtcgt cgtgtgcgcc
    10141 ggaccgccgg cggcggacgc cgggctgcgc gacctgctcg ccgccggcgc ggaccgggca
    10201 gtgcgcgtgg acctgcccgg tgccgacgct gcggacgtgg gggcccagac cggcgagggg
    10261 gccgccgcgc tcctggcccc ggtgctctcg gagctcggcg cgtccgtcgt cgtgtgcggc
    10321 gacgtcagcg cggacctcgg ttcgggcacc gtcccggcct acctggccca ccacctgggg
    10381 gccgcccagg ccctcggact gctcgacgtc accgtctccg gctcgggtca cctccgggcc
    10441 gtccgacgcc tcgacggcgg ccgacgcgag gtgctcgagg tcgccgcccc ggcggtgctg
    10501 tcggtggagg gcggcgtggc cgaactgcgc cgcgctccgc tgtcggcgac gctcgtcgcc
    10561 ggtgacgccc ccgtcgaggt caggcccggt cgggaacgcc acgtcggcga cggaccccga
    10621 ctgcgtccgt ggcgcccacc gacccgggtg gtcgagccgc cggccggtga gcacgccctc
    10681 gaccggatcg tgtcgctcac cggcgcgctg gtcgaccgga cgccgccgcg gaccgtggag
    10741 ctcgatccgg ccgccgcggc ggccgcgatc gtcgagcagc tccgggcctg ggggcacctc
    10801 ggcgccgacc gtgatcccga ccccggaaac ggggcggctg cgggaggctg agcgggtgcg
    10861 cctggcggag ctgagctggc ccgacgtccc ggagccgtcc gtgctggtgg tgccgctcgg
    10921 cagctgcgag cagcacgggc cgcacctgcc gctcgacacc gacacccgca tcgccgaggc
    10981 gctcgccgcg gccctcgtcg accggctcgg cgacgggatg gtgctcggcc cgaccgtcgg
    11041 catcggcgcg tcaggggagc acgagagctt ccccggcacg ctgtcgatcg gaaccgtcgt
    11101 gctcgagggc gtcctcgtcg agctcgtccg ctcggcgctt ccaccaccgg gctcggaccg
    11161 gccccgaccg ttccgcgggg tggtcgtcgt caacggtcac ggcgggaacg tcgaggcgac
    11221 cacccgggcc gcggcgctgc tcaccgacga gggccgcgac gtgctggtct ggcatccccg
    11281 cgttcccggg ggcgactccc acgccggtgc gaccgagacg tccatgctgc tgcacctgtg
    11341 ccccgaggtg gtcgacctca cccgcgccga ggtcggttcc accgcccgct ggcgcgacat
    11401 cggcgacgcg gtgcggaccg gtgggctggc cgcggtgagc gccaacggcg tgctcggcga
    11461 cccgaccggc gccaccgagg aggaggggcg gcggatcgtc gggatgctcg tcgagcagct
    11521 gtgcgccgcg gtcgaccgtt ggcgggacac actggagcgg tgagcgtgga tcagtcccca
    11581 cccccggcgc gcgtcgccgg cccgtcgacc gggcgcggca ccgcgctggt caccggagcg
    11641 gcgcggggca tcggtgccgc ggtggcggcc cgcctcgcgg ccgagggcta ccgggtcgtc
    11701 ctggccgatc ggtgcagcgg gccggactcc gatgtcgtgc tcggctaccg gttggcgagt
    11761 cgtgacgagc tcgacgccac cgcagccgcg atcggcccgg cggcgacggg gatcgagctc
    11821 gacgtgcgcg acctcgacgc ggtccgacgg gtggtctcca ccgtggagga cctgcgggtc
    11881 gtggtcaccg cggccggggt ggtctggggc ggggcgccgg tgtgggacat gccgggagcg
    11941 gcgtggcagg aggtgttcga cgtcaacgtc accggtacct acggggtgat cgccgcggcg
    12001 gtgccgcggc tgttggatca acctgaaccc cggaccggtc ggatcgtcgc gctcgcctcg
    12061 gccggggcgt cgcgtggcct gccccacatg gcggcctact cggcggccaa gcacgcggtg
    12121 gtcggcctgg tccgctcggt cgccggcgag ctcgccgacc ggggcgtcac catcaacgcc
    12181 gtcgcccccg gctccacccg caccccgatc ctgcgggcgt cggccgaggc gtaccgcctg
    12241 ggggcggtcg aggagttcat cccgcaccag ccgatcggac ggctgctcga cccgtcggag
    12301 atcgcggacg ccgtcgcgtg gctctgctcg ccgggtgccg ccgcggtgac cgggtccgtc
    12361 gtcgcggtcg acggcggcat gaccgcggtc tgacgtgcgg ttccgggcgg accgctcgct
    12421 gcggatcctc gaccgagccg gcgagagggt ccccgtcctc ggtggctctc cgctgcggct
    12481 gtggcgtctc accggggccg gaggtgagct cgtcgctcgg ttggccgccg gtgacgacgt
    12541 ggtcgtcgcg gcaggttcgc cgaccgctgc gctggtcgac cggctgctcg acgccggtgc
    12601 gctgcacccc gacccgacga gcctgccctc gaggtgttcc ccgagcgacg tcaccgtggt
    12661 cgtcccggtc cgtgaccgtc ccgacgcgct cgacgggctg ctcgcgtcgc tggggccgtt
    12721 ccacgctgcc ggggccggac tggtcgtcgt cgacgacgga agcgtcgacg gcccctcgca
    12781 cgagcgggtg tcgcagcgac acggtgccac ctacatccgc cgggagcgat ccggcggtcc
    12841 gggtgccgcc cgtgacgacg gcatcgccac ggcgacgacc ccgctcgtcg cggtcgtcga
    12901 cagcgactgc gtcgtgccgc ccggggcggg gcactggtgg gagccactac tgcggctgtt
    12961 cgacgacgat cgcgtcgccg ccgtcgcacc ccgggtgcgc acaccgccgg ggcccgctct
    13021 gctggcgtgc tacgaggggc tccgctcacc gttggacatg ggagcggatc cggcccgggt
    13081 gagtcccggg acccgcgtct cctatgtgcc ggccgccgcc ctggtgctgc gcaccgacgc
    13141 ccatcgggcc gtcggcgggt tcgaccccga cctcagcgtc ggcgaggacg tcgacctgat
    13201 ctggcggttg gccgacgccg gtcaccggat ccgctacgaa ccgacagcgg tgctcgacca
    13261 tccgccgcgc cgggggctgg gggcgtggat gcgccagcgg gtcggctacg gctcgtcggc
    13321 cgggccgctc gacgcccgcc acccctggcg ggtggcaccg gtgcgctgct cgccgtggag
    13381 cgcggcgggc tgggcggcga tcgtgctcgg tcccgggtcg gccgggccga tcgcgggggc
    13441 ggcgatcctg gggatcagcg ctgcgctgct gccccgacgc ctcgccgacg tgcggaccgg
    13501 cgctgcagtc cgactcgcgg tgtccggcca cggcgccgcc ggcgtgcagc tggctcgggc
    13561 cgcggtgcgg gtgtggt
//
LOCUS       DAOQOD010000080        13530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1611_length_13530_cov_11.104935, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000080 DAOQOD010000000
VERSION     DAOQOD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1611_length_13530_cov_11.104935"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..938
                     /locus_tag="PLZ72_09165"
     CDS             102..938
                     /locus_tag="PLZ72_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA36504.1"
                     /translation="MTEPTAHPDPVEVPGPLVRHERTDAGVDVLTLSHGKVNALSSAV
                     LAELRDRVGELRAAGARAVVVTGGPKLFAAGADITEFAERGGDEPFAVAAPERVAEIG
                     AGFLDALEDLAALPCPTIAAVNGVALGGGCELALACDLRVASDRARFGQPEILLGIIP
                     GGGGTQRLARLIGPARAKDMVLTGRTVDAAEALDFGLVDRVVDGDALAPALELATALA
                     QGPAAALALAKRAVDGGLDGTLSDGLRLEQRLFVDSFATGDAAVGVRSFLRDGPGKAQ
                     FP"
     gene            complement(1010..3616)
                     /gene="glgP"
                     /locus_tag="PLZ72_09170"
     CDS             complement(1010..3616)
                     /gene="glgP"
                     /locus_tag="PLZ72_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNA36505.1"
                     /translation="MRALRSFTVRPRLPGGLEPLDRLAGNLRWSWDRPTRELFTGIDP
                     RVWDEGGHDPRRVLAETPTARLEELAADPAFLERLSAVTAALDAYESLPRWFQAQLAK
                     GAIPATGGESDLDGGVVAYFSPEFGIAEAVPQYSGGLGVLAGDHLKAASDLGVPLVAI
                     GLFYRHGYFRQSLTVDGWQQERFPDLDPYAMALELCDGVRISVELGEQTLIAQVWRAS
                     VGRTPLYLLDADIEENAQDLQLVTDRLYGGDVEHRLRQEILLGIGGVRALEALGVPAH
                     VFHTNEGHAGFLGLERIRMLMQRDGLSFAEALEAVRSGAVFTTHTPVPAGIDQFPREL
                     IEKYFGRWASECGIGLDELMSLGHRPGADPSDRFNMAVMGMRLAERRNGVSALHGEVS
                     RAMFHDLWPGVPESETPVGHITNGVHGATWVSSEMAGLFTDSVGIDWQWADPEAWEQV
                     RTVDDDVLWEAHRSAKVRLVEHVRERLRTSGTSLGRSASELAWVDDALDPDALTICFA
                     RRFATYKRAALLLSQADRLRALLGDEDRPIQFVFAGKAHPADDSGKELIRQIVTFSHD
                     VAVRTRFVFVEDYDMALARSLVQGADIWLNTPVRPMEASGTSGMKAVMNGAMHCSVLD
                     GWWAECFRAGSDGRDDSHESDGPNGWAISSAESVDDDARRAELEANSLFDILETQIVP
                     TYHSAGPGSTPTEWVRVMKESLRTLAPFVSAHRMVRDYTSTLYLPAASRAVELAADGY
                     SAARELAGFRSRLDALWHQVHVDSVDVDESVADLGGTRRVTATVALGELGPDEVEVQL
                     VAGQVGQSGELEDTVTVSMAEEGSAPDGHRVFAADAPILSAGRMGVNVRVVPRSRLVD
                     TPLELGRVAWAG"
     gene            3738..5294
                     /locus_tag="PLZ72_09175"
     CDS             3738..5294
                     /locus_tag="PLZ72_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36506.1"
                     /translation="MSRRPLLVALAVLGAVVVVATATGPVRAQGVDPSTTTVPGPGSS
                     TATTGTTDPSGPVAYATADGRVWVGQGDGAPIEVAQGAAVGRGGQAAVAIAPTADLVA
                     FVRADHTLVTVPVDGGPPTVLASDAATTSLGRDPSIAWDGTGSTLAYLAVGTADMAEP
                     RPSVPAPLHDPSWFRVPLPVGVLGNVVKIVQRDGTPISRLGDPSVRSYVGVTASPADG
                     LLLLESVIPGTDQRYTLVGATAEKETPTYFSADDPAFAPDGRFIVAAGPAKGRRELIR
                     VDAQTLDRVTLVRDDSICNPSVSPDGTRIVYGGGRNCSRLMLISSKGGRAIDITPPET
                     PDTATFGVSALGWTSEGRWVTSADCRNDAGRVSCDGPVLFLNPDSGRVRSGPTATTVA
                     PVQRPLVQDVYLDIDLRGPIEFNHSFLLDPKVQGEVTGGDGEPGRLKARLVDGETVME
                     VDLMTEGGTSVSGRITITDPASGVDRSFMVLGRPTVLGVRVLSISGIWVTTQDLPWAT
                     GRFDMAVRRR"
     gene            5291..7339
                     /locus_tag="PLZ72_09180"
     CDS             5291..7339
                     /locus_tag="PLZ72_09180"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HNA36507.1"
                     /translation="MTPPLGRIMFRDLSPVTPGGFPARGVAGDPVPVAVTLVRDGHGL
                     LAGRVRWRPIGATAWDVAPLADRMDGSGRWVGRFTPGAPGMYEFEIQAWWDRFATWRR
                     DLRERAAAAQDLTVEFEVGARLIESMLDLVDPPARDRLGDAVSGLRSASCSEQVRLAV
                     GLDDAVAGLLDGVPDPVETVASSRHRLRVDRERAVFGSWYELFPRSEGGFVPRARTWD
                     RLEAVAAAGFDVLYLPPIHPIGLSHRKGPDNSLVAGPGDVGSPWAIGLAGTGAPAADG
                     GLPVGAGGHDSLHPELGTLADFDRFVARAAELGVEVALDVAFQCSPDHPWAAEHPEWF
                     TRLPDGTIRYAENPPKKYQDIYPINFWPDRDQDRVALWEACRGVFETWAERGVRIFRV
                     DNPHTKPLAFWAWVIPELLERWPDLVLLAEAFTLPSMMHALAEVGFHQSYTYFTWRTS
                     TWDLRSYGEELVGGPAAGWFRPNFWPNTPDILSGPLRDGTLAAFALRAVLAATLSPSW
                     GVYSGFELGENAPASEDNEEYLHSEKYQLVERDWDRSDSLMPLLATLNRIRREHPSMQ
                     RIDSLRFLDVDGEDLLAYTHHVPAGTVVGGRVLGGDDTVICVVNLHPTEAREGIVHLD
                     PAALGLPAGAPYRVLDELDGTTYTWGGSANYVRLDPAERPAHILAPSRSVRCFTTPAL
                     "
     gene            7409..9097
                     /gene="treS"
                     /locus_tag="PLZ72_09185"
     CDS             7409..9097
                     /gene="treS"
                     /locus_tag="PLZ72_09185"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNA36508.1"
                     /translation="MMQPPSTGSLPVADPEWFRRAVFYEVHVRGFYDATGDGNGDLRG
                     LTDKLDYLEWLGIDCLWLLPFYSSPLRDGGYDISDFYQVHPDFGTTEDAARLIEEAHR
                     RGIRVIADMVVNHTSDAHPWFQESRRDATNPKADWYVWGDDDQRWSEARIIFTDTEPS
                     NWSWDPVRGQYYWHRFFHHQPDLNYRNPEVQQAMLDVVTHWLGLGLDGFRLDAVPYLF
                     ERDGTNGENLPETHEYLRRLRKEVDAAFPGKVLLAEANQWPEDVVEYFGDGDECHMCF
                     HFPLMPRMFMSLRREESTPVAEILARMPQIPDGCQWGIFLRNHDELTLEMVTDEERDY
                     MYAEYASDPRMRRNVGIARRLFPLLENDRRQAELLHALLFSMPGTPILYYGDEIGMGD
                     NIYLGDRDGVRTPMQWNPDRNGGFSTADFAQLYLPPLMDPVYGFQALNIEGERRDGAS
                     FLNWLRRIIHVRQQHALFGTGGFEILEVSTPSVFAYLRTPPEGADPSVRPVLCVNNFS
                     GKAQPAELFLSHRAGSVPVELMGGVSFPAIGELPYFVTLPPYGFLWFELTEPAS"
     gene            9219..10535
                     /locus_tag="PLZ72_09190"
     CDS             9219..10535
                     /locus_tag="PLZ72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36509.1"
                     /translation="MAGQRWFAAVDDPRVRIRRVDTLVDGWPSLLWVLAEVRADGGRG
                     DSTWYQLPLGAASECPDELPDVAHVGRLETPRGEAHLFDALADEELALRFCSIVAPGL
                     DVRTVRAQAGEQSNTSLVFDERYILKVFRRVQPGPNPDVEVTEALGRIGFGAVPVPVA
                     VWRRSNTDFAVTRRYERSRGDGVELATASLREMFNRRRPPRDCKLDFAKEAYQLGRDV
                     AALHVALAEAFGSEPADGAAWAADMTAQLHRVASGHLETQRIEEVYQRLSTADDLGRA
                     IRIHGDLHLGQVLRLARSWMVLDFEGEPDRPVDERRRPSSPLRDVAGMTRSFHYAAGM
                     ALREMESPDRELRLLADAWAERAVNTFLSGYADVDDVHRLLPQARASRDALLSVFEMD
                     KAVYEVAYELAHRPDLVDLPIRAVERLLDYDDELPGAPPDESDEPA"
     gene            10532..12517
                     /gene="glgB"
                     /locus_tag="PLZ72_09195"
     CDS             10532..12517
                     /gene="glgB"
                     /locus_tag="PLZ72_09195"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNA36510.1"
                     /translation="MNAAPGPDPAPPGDVDLYLFNEGRHRRLWELLGAHALPGGGATF
                     AVWAPNADRVRVVGDWEHWSDADGDALDLAPIASSGIWWGVEPRAGVGHTYKFEVAGA
                     DGRVTLRADPLARQAEVPPRNASVIAADGAVARSLGVASPWSEPADSWRRARAERNDG
                     RLSVYEVHLGSWRRHPDGSSHTARQLAGPLADWAVELGFTHVELLPVATHPFGGSWGY
                     QVSGYYAPDARLGSPDDLRHLIDTCHDRGLGVIVDWVPAHFPRDEFALARFDGTALYE
                     HADPRRGEHPDWGTLVFNHGRLEVRNFLVANALYWLEEFRVDGLRVDAVASMLYLDYS
                     RRAGEWLPNEHGGREDLDALAFVRELTDVVHDEHPGALVVAEESTSWPGVTAPTAWDG
                     LGFDRKWNLGWMHDSTGYFRHDPVHRSHHHHELTFPMVYAGHERWILPLSHDEVVHGK
                     GSLLAKMPGDEWQRFANLRALLAWQWVHPGRQLLFMGGELAQEQEWSHDRELDWWLLQ
                     HPAHLGVRTLVGDLNRLQADTPALWAGDDDLDGNFWWLDADDRDHSVFAFGRRIPDGH
                     PSRDPAAGQPAPAEVVVVVANLTPVPRPGYRLGVPFEGDWRVLLDTDARRYGGSGHEL
                     VPDGGAVLAADVDTPWQGQPFSLRLTLPPLAVVALSI"
     gene            12581..13459
                     /locus_tag="PLZ72_09200"
     CDS             12581..13459
                     /locus_tag="PLZ72_09200"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNA36511.1"
                     /translation="MTDAPGPPTAEPPRLTVCYRHPDRAGGVGCQRCGRPICVQCMVP
                     ASVGYQCPECTHARPQKVVSGRAAFGGGGADVVVGKVLVALNVALYALTVVVGGSTSA
                     RGAVYEHLVTFGPAVAAGEWWRLVTGGFMHATPLHLLMNMVLLWLLAKELEPVLGHLS
                     FGLLYAVSLLGGALGVMLMSPNDPTLGASGAVFGLMGALVVLQLRARQNPWHTGLGGL
                     VLINLVLTFAIPGISVGGHVGGLLAGAAAGLIVVPRQTPETTVGIRHAFLALTALGLG
                     ALAVVAAGVLAVGGWY"
BASE COUNT         1792 a         4990 c         4809 g         1939 t
ORIGIN      
        1 gacgcctgcg aacccgacgt cgcccggcgg gtcgggctgg ccgacgccct gcgtcgcctt
       61 gacgccgagc ggcgacgacg ggaccggtac attccgggcc catgaccgaa cccaccgccc
      121 acccggaccc cgtcgaggtg cccggcccgc tggtccgaca cgagcgcacc gacgccggag
      181 tcgacgtgct caccctctcc cacggcaagg tcaacgccct cagcagcgcg gtcctggcgg
      241 agctgcgcga ccgcgtcggc gagctccgag ctgccggcgc ccgcgccgtg gtggtgaccg
      301 gcgggcccaa gctgttcgcc gccggcgccg acatcaccga gttcgccgag cgcggcggcg
      361 acgagccgtt cgcggtggcc gcgccggagc gggtggccga gatcggcgcc gggttcctcg
      421 acgcgctcga ggacctggcc gccctgccct gtccgacgat cgccgcggtc aacggcgtcg
      481 ccctgggcgg cggatgcgag ctggccctgg cctgcgacct gcgcgtggcc tcggaccggg
      541 cccggttcgg ccaacccgag atcctgctcg ggatcatccc cggcggaggc ggcacccagc
      601 gcctggcccg gctgatcggc ccggcccgcg ccaaggacat ggtgctcacc ggtcggaccg
      661 tcgacgccgc cgaagccctg gacttcggcc tggtcgaccg ggtggtcgac ggcgacgccc
      721 tggccccggc gctcgagctg gccaccgcgt tggcacaggg gcccgccgcc gcgctggcgc
      781 tggccaagcg ggccgtcgac ggcggcctcg acgggaccct gtccgacggg ctgcggttgg
      841 agcagcgcct gttcgtcgac tcgttcgcca ccggtgacgc ggcggtcggc gtgcggtcgt
      901 tcctgcgcga cggccccggg aaggcccagt tcccctgatc cccggcagtg ccgttggtca
      961 gccggcccag gcagtgctgc cggtcagcca gcccaggcga cgccgttggt cagccagccc
     1021 aggcgacgcg ccccagctcc aggggggtgt cgaccagccg actgcgcggg accacccgca
     1081 cgttcacgcc catgcgcccg gcgctgagga tgggggcatc ggctgcgaag acgcggtggc
     1141 cgtcgggagc ggagccctcc tcggccatcg acacggtgac ggtgtcctcg agctcgccgg
     1201 actgaccgac ctgcccggcc accagctgca cctcgacctc atccggaccg agctcgccca
     1261 gcgcgacggt ggcggtgacc cggcgggtgc cgcccaggtc cgcgaccgac tcgtcgacgt
     1321 cgaccgagtc gacgtggacc tggtgccaga gggcgtccag gcgggaccgg aacccggcca
     1381 gctcccgggc cgcggagtag ccgtcggcgg ccagctcgac cgctcgggac gcggcgggca
     1441 ggtacagcgt cgaggtgtag tcccgcacca tccggtgcgc gctgacgaac ggggcgagcg
     1501 tgcgcaacga ctccttcatc acgcgcaccc actcggtcgg ggtggagccg ggtccggccg
     1561 agtggtaggt cggcacgatc tgggtctcga ggatgtcgaa caggctgttg gcctcgagct
     1621 cggcccggcg ggcgtcgtcg tcgaccgact cggccgacga gatcgcccag ccgttcggtc
     1681 cgtcgctctc atggctgtcg tcgcggccgt cggagccggc gcggaagcac tcggcccacc
     1741 agccgtcgag caccgagcag tgcatcgccc cgttcatgac cgccttcatg ccgctggtcc
     1801 ccgacgcctc catcggccgc accggcgtgt tgagccagat gtcggcgccc tgcaccaacg
     1861 accgggccag ggccatgtcg tagtcctcga cgaacacgaa gcgggtccgc accgcgacgt
     1921 cgtgggagaa cgtgacgatc tggcggatca gctccttgcc gctgtcatcc gcgggatgtg
     1981 ccttgcccgc gaacacgaac tggatcggac ggtcctcgtc gccgagcagg gcacggaggc
     2041 ggtcggcctg gctgagcagc agggcggcgc gcttgtaggt cgcgaaccgc cgggcgaagc
     2101 agatcgtcag ggcgtcgggg tcgagggcgt cgtccaccca ggccagctcc gacgcggacc
     2161 gtccgagcga cgtgcccgag gttcggagcc gctcccggac gtgctcgacc agccgcacct
     2221 tcgccgagcg gtgcgcctcc cacagcacgt cgtcgtcgac ggtccggacc tgctcccatg
     2281 cctccggatc cgcccactgc cagtcgatgc cgaccgaatc ggtgaacaga cccgccatct
     2341 ccgacgagac ccaggtcgcg ccgtgcacgc cgttggtgat gtggccgacc ggcgtctcgg
     2401 actcggggac gcccggccac aggtcgtgga acatcgcccg gctgacctcg ccgtgcagcg
     2461 ctgacacccc gttgcgtcgc tcggccaggc gcatgcccat caccgccatg ttgaagcggt
     2521 ccgaggggtc cgcccccgga cggtgaccga gcgacatcag ctcgtccaga ccgatcccgc
     2581 actcggaggc ccagcggccg aagtacttct cgatcagctc ccgtgggaac tggtcgatgc
     2641 cggccgggac cggggtgtgg gtggtgaaca cggcgccgga gcggacagcc tccaacgcct
     2701 cggcgaagga caacccgtcg cgctgcatca gcatccggat gcgctccagg cccaggaaac
     2761 cggcgtggcc ctcgttggtg tggaagacgt gagcgggcac gccgagcgcc tcgagcgccc
     2821 ggacgcctcc gatgccgagc aggatctcct ggcggagccg gtgctcgacg tcgccgccgt
     2881 agagccggtc ggtgaccagc tggaggtcct gggcgttctc ctcgatgtcg gcgtcgagca
     2941 ggtacagcgg cgttcggccc accgaggccc gccacacctg ggcgatcagc gtctgctcgc
     3001 ccagctccac cgagatccgc acgccgtcgc acagctcgag cgccatggcg tacgggtcga
     3061 ggtcggggaa ccgctcctgc tgccagccgt cgacggtgag cgactggcgg aagtagccgt
     3121 gccggtagaa caggccgatg gcgaccagcg ggacccccag gtccgacgcc gccttgaggt
     3181 ggtccccggc caggacgccc aggccgccgg agtactgcgg gacggcctcg gcgatgccga
     3241 actcgggcga gaagtaggcg accacgccgc cgtcgaggtc ggactccccg ccggtggcgg
     3301 ggatcgcacc cttggcgagt tgggcctgga accagcgcgg gagcgactcg taggcgtcga
     3361 gcgcggcggt caccgccgag aggcgctcga ggaacgccgg gtcggccgcc agctcctcca
     3421 gccgggccgt cggcgtctcg gccaacacac ggcgggggtc gtgtccgcct tcgtcccaga
     3481 cccgcggatc gatgcccgtg aacagctccc gggtggggcg gtcccaggac caccgcaggt
     3541 tcccggccag gcggtcgagc ggctcgagcc cccccggcag tcgggggcgg acggtgaagg
     3601 aacgcagcgc tctcacacac cggcagggta gcgccggtgc gggtccgccc caccgggagg
     3661 gacctgaggg cgctcggggg gttgccggcg cagtcgcggg agggcgacgg cgacccgccg
     3721 gtagcctggg acaacgcatg tcccgccgtc cgctgctcgt cgcgctcgcc gttctcggcg
     3781 ccgtggtcgt ggtcgccacc gcgaccgggc cggtgcgcgc ccagggcgtc gacccatcca
     3841 ccaccacggt gcccggaccg gggtcgtcga cggccacgac cggaacgacc gacccgtccg
     3901 gaccggtcgc ctacgccacc gccgatggcc gggtgtgggt gggccagggc gacggcgccc
     3961 cgatcgaggt cgcccagggt gccgccgtgg gtcggggagg acaggcggcg gtcgccatcg
     4021 ctcccaccgc cgacctggtc gccttcgtga gggcggacca caccctggtg accgtgccgg
     4081 tggacggcgg gccgcccacg gtgctcgcct cggacgcagc gaccacctcg ctcggccgtg
     4141 acccctcgat cgcgtgggac gggaccgggt ccaccctggc ctacctggcg gtcggaaccg
     4201 ccgacatggc ggagccccgg cccagcgtcc ctgcacccct gcacgacccg tcctggttcc
     4261 gggtgccgtt gccggtcggg gtgctcggca acgtcgtgaa gatcgtccag cgcgacggca
     4321 ccccgatctc ccggctcggc gacccgtcgg tccgctccta cgtcggggtg accgcctcgc
     4381 ccgccgacgg gttgctgctc ctcgagtcgg tcatccccgg caccgaccag cgctacaccc
     4441 tggtcggtgc cacggccgag aaggagacgc cgacgtactt ctcggccgac gaccccgcct
     4501 tcgcccccga cggccgtttc atcgtggccg ccggaccggc caaggggcgt cgggagctga
     4561 tccgcgtcga cgcccagacc ctggaccggg tgacgctggt ccgcgacgac tcgatctgca
     4621 acccgtcggt gtcgccggac gggacgcgca tcgtctacgg cggcggccgc aactgctcac
     4681 gactgatgct gatctcgtcc aagggcggcc gcgcgatcga catcacccca cccgagaccc
     4741 ccgacaccgc caccttcggg gtgtcggccc tgggctggac ctccgagggt cgctgggtga
     4801 cctcggcgga ctgccgcaac gacgccggcc gggtctcctg cgacggtccc gtgctgttcc
     4861 tcaaccccga ctcgggccgt gtccgctccg ggccgacggc cacgacggtc gccccggtgc
     4921 agcgtccgct ggtgcaggac gtgtacctcg acatcgacct gcggggtccg atcgagttca
     4981 accactcgtt cctgctggac cccaaggtgc agggcgaggt caccggtggc gacggtgagc
     5041 cgggtcggct caaggcccgg ctggtcgacg gcgagaccgt gatggaggtc gacctgatga
     5101 ccgagggcgg gacgtcggtg tcgggtcgca tcacgatcac cgacccggca tccggcgtgg
     5161 accgctcgtt catggtgctc ggccgtccga ccgtgctcgg cgtgcgggtc ctgtcgatca
     5221 gcgggatctg ggtgacgacc caggacctgc cgtgggcgac cggtcgcttc gacatggcgg
     5281 tccggcgccg gtgaccccgc cgttggggcg gatcatgttc cgggacctgt cgccggtcac
     5341 ccccggcggg ttccccgccc gcggcgtcgc cggcgacccg gtgccggtgg cggtgaccct
     5401 ggtccgcgac ggccacggcc tgctcgccgg acgcgtccgc tggcggccga tcggcgccac
     5461 ggcgtgggac gtcgccccgc tggcggaccg catggacggc tcggggcgct gggtcgggcg
     5521 attcaccccg ggggctccgg ggatgtacga gttcgagatc caggcctggt gggaccgctt
     5581 cgccacctgg cgtcgcgacc tgcgcgagcg ggccgccgcg gctcaggacc tgaccgtgga
     5641 gttcgaagtc ggcgcccgcc tgatcgaatc gatgctcgat ctggtcgacc cgccggcccg
     5701 ggaccgcctg ggcgacgccg tgtccgggct ccggtccgcg tcgtgctcgg agcaggtgcg
     5761 tctcgccgtc ggtctcgacg acgcggtcgc cgggctgctc gacggggtcc ccgacccggt
     5821 cgagaccgtc gcgtcgagca ggcaccgcct gcgcgtcgac cgggaacggg cggtgttcgg
     5881 cagctggtac gagctcttcc cccgatcaga gggcggcttc gtccccagag cgcgcacgtg
     5941 ggaccggctc gaagcggtgg ccgccgcggg gttcgacgtg ctgtacctgc cgccgatcca
     6001 cccgatcggg ctgagccacc gcaagggtcc ggacaactcg ctggtggccg gtcccggcga
     6061 cgtcggcagc ccgtgggcga tcggcctcgc cgggacgggc gcgccggcgg ccgacggcgg
     6121 tctgccggtc ggagcgggcg gccacgactc gctgcacccc gagctgggga cgctggcgga
     6181 cttcgaccgc ttcgtcgccc gggccgccga gctcggtgtc gaagtggcgc tcgacgtcgc
     6241 cttccagtgc agccccgatc acccgtgggc cgccgagcac cccgagtggt tcacccgcct
     6301 gcccgacggc accatccgct acgccgagaa cccgccgaag aagtaccagg acatctaccc
     6361 gatcaacttc tggcccgatc gcgaccagga ccgcgtcgcg ctgtgggagg cgtgccgcgg
     6421 cgtgttcgag acgtgggccg agcggggggt gcggatcttc cgggtcgaca acccgcacac
     6481 caagccgctg gcgttctggg cgtgggtcat ccccgagctg ctggagcgtt ggcccgacct
     6541 ggtgctgctg gccgaggcgt tcaccctgcc gtcgatgatg cacgccctcg ccgaggtggg
     6601 cttccaccag agctacacgt acttcacgtg gcggacctcg acctgggacc tgcgcagcta
     6661 cggcgaggag ctggtcggtg ggccggccgc ggggtggttc cgcccgaact tctggcccaa
     6721 cacgcccgac atcctctcgg gaccgctgcg cgacgggacg ctggccgcgt tcgctctgcg
     6781 ggcggtgctc gccgccaccc tgtcgccgag ctggggtgtg tactcgggct tcgagctggg
     6841 cgagaacgcg ccggcgtcgg aggacaacga ggagtacctc cacagcgaga agtaccagct
     6901 ggtcgaacgg gactgggacc gttcggattc gctgatgccg ctgctggcca cgctcaaccg
     6961 gatccgtcgc gagcacccgt cgatgcagcg gatcgactcg ctgcggttcc tcgacgtcga
     7021 cggcgaggac ctgctggcct acacccacca cgtcccggcg gggaccgtgg tcggcggtcg
     7081 ggtcctcggc ggcgacgaca cggtcatctg cgtggtcaac ctgcacccca ccgaggcccg
     7141 ggaggggatc gtccacctgg atcccgctgc cctcgggctc cctgccggtg cgccgtaccg
     7201 ggtgctcgac gagctcgacg ggaccaccta cacgtggggc gggtccgcca actacgtgcg
     7261 actcgacccc gccgagcgcc cggcccacat cctcgccccc tcccgttctg ttcgttgttt
     7321 cacaacgcct gcgttgtgaa acaacgaaca gaacggggag cgcgggggag cggtgggaag
     7381 cgggggcgcc ggtacgctgg agcgcgccat gatgcagcca ccgtccaccg ggtccctccc
     7441 cgtcgccgat cccgagtggt tccgccgagc cgtcttctac gaggtccacg tccgcggctt
     7501 ctacgacgcc accggtgacg gcaacggcga ccttcgcggt ctcaccgaca agctcgacta
     7561 cctggagtgg ctcggcatcg actgcctgtg gctgctgccg ttctactcgt cgccgctgcg
     7621 cgacggcggc tacgacatct cggacttcta ccaggtgcac cccgacttcg gcaccaccga
     7681 ggacgccgcc cggctgatcg aggaggccca ccgccggggc atccgggtga tcgccgacat
     7741 ggtcgtcaac cacacctccg acgctcaccc ctggttccag gagtcgcgcc gggacgccac
     7801 caaccccaag gccgactggt acgtgtgggg cgacgacgac cagcgctggt ccgaggcccg
     7861 catcatcttc accgacaccg agccgtccaa ctggagctgg gacccggtgc gcggccagta
     7921 ctactggcac cggttcttcc accaccagcc cgacctcaac taccgcaacc ccgaggtcca
     7981 gcaggcgatg ctggacgtgg tgacccactg gctgggactc ggcctcgacg ggttccgcct
     8041 cgacgccgtg ccctacctgt tcgagcgcga cggcaccaac ggcgagaacc tgcccgagac
     8101 ccacgagtac ctgcgccggc tccgcaagga ggtcgacgcc gcgttccccg gcaaggtgct
     8161 gctcgccgag gccaaccagt ggcccgagga cgtcgtcgag tacttcggcg acggcgacga
     8221 gtgccacatg tgcttccact tcccgctcat gccccgcatg ttcatgagcc tgcggcgcga
     8281 ggagtccacc ccggtcgccg agatcctggc ccgcatgccg cagatccccg acggctgcca
     8341 gtggggcatc ttcctgcgca accacgacga gctcaccctg gagatggtga ccgacgagga
     8401 gcgggactac atgtacgccg agtacgccag cgacccgcgc atgcggcgca acgtgggcat
     8461 cgcccggcgg ctgttccccc tgctcgagaa cgaccgccgc caggccgagc tgctgcacgc
     8521 cctgctgttc tccatgcccg gcacaccgat cctctactac ggcgacgaga tcggcatggg
     8581 cgacaacatc tacctgggcg accgcgacgg cgtgcgcacg cccatgcagt ggaacccgga
     8641 ccgcaacggc gggttctcca ccgcggactt cgcccagctc tacctgccgc cgctgatgga
     8701 tccggtgtac gggttccagg cgctcaacat cgagggcgag cgtcgggacg gggcgtcgtt
     8761 cctgaactgg ctgcgccgca tcatccacgt ccgccaacag cacgctctgt tcggcaccgg
     8821 cggcttcgag atcctcgagg tgtcgacccc gtcggtgttc gcctacctgc gtaccccacc
     8881 cgagggcgcc gacccgtcgg tccgcccggt gctgtgcgtc aacaacttct cgggcaaggc
     8941 ccagcccgcc gagctgttcc tgtcgcaccg cgccggcagc gtgccggtcg agttgatggg
     9001 cggggtgtcg ttccccgcca tcggcgagct gccctacttc gtcacgctgc ctccctacgg
     9061 cttcctctgg ttcgagctca ccgaaccggc ctcctgaccc cgtgacgagg ggcctcccgg
     9121 cggtggcggg ttcgaaccgg cccggctcga gcgggtagaa acgacacatc gacgctggtt
     9181 cattctccgc cgagctcgcc cgcctgctgc ctccgttcct ggccggtcag cggtggttcg
     9241 ccgcggtcga cgacccccgg gtccgcatcc gccgggtcga cacactcgtc gacggctggc
     9301 ccagcctgct gtgggtgctc gccgaggtgc gcgccgatgg cggccggggc gactcgacgt
     9361 ggtaccagct cccgttgggt gccgcgtcgg agtgccccga cgagctcccc gacgtcgccc
     9421 acgtcggacg gctcgagacc ccccggggcg aggcgcacct cttcgacgcc ctggccgacg
     9481 aggagctggc cctgcggttc tgctcgatcg tggcccccgg actcgacgtc cggacggtcc
     9541 gggcccaggc cggcgagcag tccaacacgt cgctggtgtt cgacgagcgc tacatcctca
     9601 aggtgttccg gcgtgtgcag cccggcccca accccgatgt cgaggtgacc gaggcgctcg
     9661 gccggatcgg cttcggcgcg gtgccggtgc ccgtcgcggt gtggcggcgc agcaacaccg
     9721 acttcgcggt cacccgccgc tacgagcgca gccgcggcga cggcgtcgag ttggccacgg
     9781 cgtcgctgcg ggagatgttc aaccgccgtc gcccgccccg ggactgcaag ctggacttcg
     9841 ccaaggaggc ctaccagctc ggacgcgacg tcgcggccct ccacgtggct ctggccgagg
     9901 cgttcggttc cgaacccgcc gacggggcgg cctgggccgc cgacatgacc gcacagctgc
     9961 accgggtcgc gtcgggtcac ctcgagaccc agcggatcga ggaggtgtac cagcggctct
    10021 ccaccgcgga cgacctcggc cgcgccatcc gcatccacgg cgacctgcac ctggggcagg
    10081 tcctgcgcct ggcccggagc tggatggtgc tcgacttcga gggcgaacct gaccgaccgg
    10141 tcgatgagcg tcgccgtccg tcgtcgccgt tgcgcgacgt ggcgggcatg acccggtcgt
    10201 tccactacgc ggcgggcatg gcgctgcggg agatggagtc gccggaccgg gagctgcggc
    10261 tgctcgcgga tgcctgggcg gaacgggcgg tcaacacgtt cctgtcgggc tacgcggacg
    10321 tcgacgacgt gcaccggctc ctgccgcagg cccgggcgtc gcgggacgcg ctgctcagcg
    10381 tgttcgagat ggacaaggcg gtctacgagg tcgcctacga gctcgcccac cgtcccgatc
    10441 tggtcgacct gccgatccgg gccgtcgaac ggctgctcga ctacgacgac gagctgccgg
    10501 gcgcgccgcc cgacgagtcg gacgagcccg cgtgaacgcc gcccccggcc cggacccggc
    10561 gccgccgggc gacgtcgatc tctacctttt caacgagggc cgccaccgcc ggctgtggga
    10621 gctgctcggc gcccacgccc tccccggtgg aggcgcgacc ttcgcggtgt gggcccccaa
    10681 cgccgatcgg gtccgggtgg tcggggactg ggagcactgg tccgacgccg acggcgatgc
    10741 cctcgacctg gcgccgatcg cctcgagcgg catctggtgg ggtgtcgagc cccgcgccgg
    10801 ggtgggtcac acctacaagt tcgaggtggc cggggccgac ggccgggtga cgctgcgcgc
    10861 cgatccgctg gcccgccagg ccgaggtgcc gccccgcaac gcctcggtga tcgccgccga
    10921 tggagcggtg gcccgctcgt tgggtgtggc atctccgtgg agcgaaccgg cggactcgtg
    10981 gcgtcgggcc cgggccgagc gcaacgacgg ccgcctgtcg gtctacgagg tgcacctggg
    11041 gtcgtggcgt cgacaccccg acgggagctc ccacaccgca cgtcagctgg ccggaccgct
    11101 ggcggactgg gcggtcgagc tgggcttcac ccacgtcgaa ctgctgccgg tggccactca
    11161 cccgttcggt ggctcctggg gctaccaggt cagcggctac tacgcccccg acgcccgtct
    11221 ggggagtccc gacgacctcc gccacctgat cgacacgtgc cacgaccggg gcctgggcgt
    11281 gatcgtcgac tgggtgccgg cgcacttccc ccgcgacgag ttcgccctgg cccgcttcga
    11341 cggcaccgcg ctgtacgagc acgcggaccc ccgacggggt gagcaccccg actgggggac
    11401 gctggtgttc aaccacggcc ggctcgaggt ccggaacttc ctggtggcca acgccctgta
    11461 ctggttggag gagttccgcg tcgacggtct gcgggtcgat gcggtggctt cgatgctcta
    11521 cctcgactac tcgcggcgag ccggcgagtg gttgcccaac gagcacggcg gccgcgagga
    11581 cctcgatgct ctggcgttcg tgcgcgagct gaccgacgtg gtccacgacg agcaccccgg
    11641 agcgctggtc gtggccgagg agtccacgtc gtggccgggg gtgaccgccc cgaccgcgtg
    11701 ggacggcctg ggtttcgacc gcaagtggaa cctggggtgg atgcacgact cgaccggcta
    11761 cttccgccac gacccggtcc accgctccca tcaccaccac gagctcacct tcccgatggt
    11821 gtacgccggt cacgagcggt ggatcctgcc gctcagccac gacgaggtcg tccacggcaa
    11881 ggggtcgctg ctcgccaaga tgcccggcga cgagtggcag cggttcgcca acctgcgggc
    11941 cctgctcgcc tggcagtggg tgcacccggg gcgccagctg ctgttcatgg gcggtgagct
    12001 ggcccaggag caggagtggt cccacgaccg tgagctcgac tggtggctgc tgcagcaccc
    12061 ggcgcacctg ggcgtgcgca ccctggtcgg cgacctcaac cggctccagg cggacacccc
    12121 ggcgctgtgg gccggcgacg acgacctcga cggcaacttc tggtggctcg acgcggacga
    12181 ccgcgaccac tcggtgttcg cgttcgggcg acggatcccc gacggccacc cgagccgcga
    12241 ccccgcagca ggacagcccg ccccggccga ggtcgtcgtg gtggtggcca acctgacccc
    12301 ggttccgcga ccggggtacc ggttgggcgt gccgttcgag ggcgactggc gggtgctgct
    12361 cgacaccgac gcccggcgct acggcggcag cggccacgag ctggtccccg acggcggagc
    12421 ggtgctcgcc gccgatgtcg acacgccctg gcagggccag ccgttctcgc tgcgcctgac
    12481 cctgccccca ctcgccgtcg tcgccctgtc gatatagggg tgctcagcga cacaccttgt
    12541 cgctttcccg acctcagaat ccggccgata ggttgacctc gtgaccgacg cgcccggacc
    12601 gccgacggcg gagccgcccc ggctgaccgt ctgctaccgc cacccggacc gagccggtgg
    12661 ggtgggctgc cagcgttgcg gtcggccgat ctgcgtgcag tgcatggtcc cggcatcggt
    12721 cggctaccag tgcccggagt gcacccacgc ccgtccgcag aaggtggtgt cgggtcgcgc
    12781 cgcgttcggc ggcggcgggg ccgatgtggt cgtcggaaag gtgctcgtcg ccctcaacgt
    12841 cgccctctat gccctgacgg tggtggtcgg agggtcgacc agcgcccgcg gggcagtcta
    12901 cgagcacctg gtcaccttcg gcccggcggt cgcggcggga gagtggtggc gcctggtcac
    12961 cggcggcttc atgcacgcca cgccgctaca cctgctcatg aacatggtgc tgctgtggct
    13021 gctggccaag gagctcgaac cggtgctcgg ccacctgagc ttcggcctcc tgtacgccgt
    13081 gtcgctgctc ggcggggcgc tgggcgtgat gctgatgtcg cccaacgacc cgaccctcgg
    13141 cgcctccggt gccgtgttcg gactgatggg tgccctggtg gtcctgcagc tgagggcccg
    13201 tcagaacccc tggcacaccg ggctgggcgg actggtgctg atcaacctgg tcctcacctt
    13261 cgccatcccc ggcatctccg tcggcggtca cgtcggcggg ttgctcgccg gagcggcggc
    13321 cgggctgatc gtcgtgccgc ggcagacgcc cgagaccacc gtcggcatcc gccacgcctt
    13381 cctggcactg accgccctcg gcctgggcgc gctggccgtg gtcgcggccg gagtcctggc
    13441 cgtgggcggg tggtactgag cccgggtcgg gctcaggggc gacggtagag cgacaccgtc
    13501 ccgtccgacg ccgggccgtc ggcaccccag
//
LOCUS       DAOQOD010000081        13457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1624_length_13457_cov_13.182435, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000081 DAOQOD010000000
VERSION     DAOQOD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_13457_cov_13.182435"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLZ72_09205"
     CDS             <1..606
                     /locus_tag="PLZ72_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36512.1"
                     /translation="PPRTGHNQPPAREEPTVSNTQPDPAQEPTPNDPADASDVPTPAQ
                     GDDDPVAEVARLRSELQKARKWEERAKTNAGAAKELEGLRKQYESDQERAVREAREAA
                     RSEVLGELGAERVADAFRVAAAGRGLDVDEVIDGINLAKFLSEDGNPDRDAVQAFVDR
                     IAPAQETAPPDLGQGARGGQQVPGLNSSQLQKDLEAALGIR"
     gene            644..>1603
                     /locus_tag="PLZ72_09210"
     CDS             644..>1603
                     /locus_tag="PLZ72_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNA36513.1"
                     /translation="MAISAPTKTSDLSGFITPAEAAPIFERAARTSVVQQLSPQVPLG
                     PSGVKIPVISTRPTAAWVGEGEQKPASAGSITPKTIEPKKLASIFVVSAEVARLNPAQ
                     FVTRMQGSFAETFAVAFDLAALHDVGPDGSAGAGPFSTYVAQTTKTQEIGATAQNAGG
                     VYGDLNAALGKVVSGKDASGRRYRVTGWALDDVVEPAIRGAVDTAGRPIWADLASEQE
                     SDLFTSGRLLGRRGFFGEGVATPDLTTTVGFGGDWGQTAWGVVGGISYRISTEASVTI
                     NGSLVSLFEHNLVAVLAEAEYGWLVNDTAAFVELTNTNNSPITS"
     assembly_gap    1604..1703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1704..2576
                     /locus_tag="PLZ72_09215"
     CDS             <1704..2576
                     /locus_tag="PLZ72_09215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA36514.1"
                     /translation="YPPSSRVGAWLSTHECLQRLAERGHEVHVRTVFAPADYTHGDVR
                     VLGRWDRSVAGYDAVVSHHGDTTSGVRQLCIKRDIPHVLMVHGTPDQVDDSEGPVVWN
                     SESSRAGRNGIVVRPHVDPAIYRTTAGDHVTLINLSREKGGDLFRQIVAAMPERKFLG
                     VRGGYGRQRIPADPNVEMVPTTTDMAADVYSRTRVLLMPSIAETWGRTGVEAMCSGIP
                     VIAHPTPGLVESLGDAGIFVDRNDLAGWVGAIRRLDDPDEYARRSAAALARAAELDTA
                     TDLDRFADYVEACA"
     gene            2573..3238
                     /locus_tag="PLZ72_09220"
     CDS             2573..3238
                     /locus_tag="PLZ72_09220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNA36515.1"
                     /translation="MIAVLIPTFGRADRLARVAANITENTETDHRVVFAVEAEDVESI
                     AAAAELDVDVVVNEGRPNYSGAITTAYRATPAEYVFAGADDLNFHPGWDTAALALMDG
                     WVQVVGTNDLLNPYVTAGLHATHCLVDRRYLDETGGVVDQGPGSFMFDGYDHQYTDTE
                     FIGTAKMRARFRPCFDSVVEHLNAWSPKGEVDATARKTMRAIDTDSELYDSRRDLWFS
                     ISR"
     gene            3235..4176
                     /locus_tag="PLZ72_09225"
     CDS             3235..4176
                     /locus_tag="PLZ72_09225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA36516.1"
                     /translation="MTGRHVVVLGGGGFIGANLCNRLYALGAHVTAVDTVFPSWRRPD
                     VMCRRADLTSLAETVRAIVGAELVFHLAADMGGVGYFHSNADLGASLVNGQITLNVAQ
                     AVEAHETPTTFYASSACAYPVELQQTPGEAPALSEDLIGHGTPDALYGAEKLHGLRIM
                     GKVPGARVGVLHTVFGPLQEHEGRRMKFPAAVATKALAARSSGTLELWGDGSQLRSYL
                     HVDDAVDRIITIATAGRYDGPVNVGAAGPVTCREIAELCLDIVGADADIVTNPAEPTG
                     VLARDCSNARYDDLYGPGPQRSYRAGFESFISWLDRL"
     gene            4173..4787
                     /locus_tag="PLZ72_09230"
     CDS             4173..4787
                     /locus_tag="PLZ72_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36517.1"
                     /translation="MRVHVGIPWRAGDPWREQAFAYVQIHLRGAGLDPVPYDNGADIF
                     SRAGSRNLAVRCADADVVILHDADMVLPAAAYLEAAELALSSDRLVIGFNRYRPLSKA
                     RTVEAFGGADPFALEPIDELVDFSVGGVLAITPECWWAAGGMDERYVDWGCEDFAFAD
                     AAGAALGPVLRVDGPAVHLWHPHAGDPTNPDQQANSRLLAGGER"
     gene            4784..5365
                     /locus_tag="PLZ72_09235"
     CDS             4784..5365
                     /locus_tag="PLZ72_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36518.1"
                     /translation="MTTRLATPDDLTRRMGITVESERALALLADASAAVISYTGQQFV
                     RATTTAQVRVPSDRKVRLGQLPVHDVDAVTDLNDNQLLFTFDGIDTVHVTVNLDSFSF
                     EPWTSPIRTVNVTYDHGFDAIPDDIVAVVCQVAGRAYGTAPQDTGAAREQLGDWSYSA
                     GAAASSGALGLLLPERAVLDRYTQRVRTIGIGP"
     gene            5362..5703
                     /locus_tag="PLZ72_09240"
     CDS             5362..5703
                     /locus_tag="PLZ72_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36519.1"
                     /translation="MRTPEHLMTRLGKIVTVADGDATDVYGNVEPTEVETTVRYELQQ
                     QRRDEAGDPDNWQIGAWSLYLPSGTAVTGWDRFIDDQNDEYELVGPPERKWNPRAQQF
                     SHVQATVRRVT"
     gene            5700..5987
                     /locus_tag="PLZ72_09245"
     CDS             5700..5987
                     /locus_tag="PLZ72_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36520.1"
                     /translation="MSRFVPAANLDVVLDHATHDDRLKVAEHMAIDARHNVNPDTGHY
                     AEEVQTFDDDRGVGVDAHDVAAHIIEFGSINNAPQAPIRRAAAEHGKFEPR"
     gene            5984..6421
                     /locus_tag="PLZ72_09250"
     CDS             5984..6421
                     /locus_tag="PLZ72_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36521.1"
                     /translation="MSLVLLPNTAALTSAFLRAQDEVADLVDDRVYTILPNSKTFPLV
                     RVRRIGGTAGTRFAHWLDAPLFQVDAWADTAAVAFEVTETCRAALVQRFSGIYHFTGV
                     SGVVTSVEAGGITEGFDPDEQTKARGRFDAVVMAHPAPDLTSS"
     gene            6508..7080
                     /locus_tag="PLZ72_09255"
     CDS             6508..7080
                     /locus_tag="PLZ72_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36522.1"
                     /translation="MAVETPEAILIAAGFEIYTAPVGTPIPTDVDSTLNAAFTRVGYT
                     TEDGAVFTRTPSVTPINMHQSFEPVRYVLAGIETTLAFTLGQWDADTIPLAFGGGTWS
                     TTANGWRFDPAEPGVIDERVVVADAVDGETKWRIVMRRVMQQGASSSTLTKSGAAGLA
                     VTLGSMAAGSGTKPWSMFTDSEYVNPVTSA"
     gene            7080..7442
                     /locus_tag="PLZ72_09260"
     CDS             7080..7442
                     /locus_tag="PLZ72_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36523.1"
                     /translation="MPDTPRTIDYSTLRLAVEYADGTRVEGIRVIPSATRAIEAEFNE
                     QFSKVATFDREDWMHTAAWTYLKLREGEDRPFDEWLATVDRLQIGVADDDRPTSGPTR
                     KSSGSSGRRSTKAKTSTD"
     gene            7701..11363
                     /locus_tag="PLZ72_09265"
     CDS             7701..11363
                     /locus_tag="PLZ72_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNA36524.1"
                     /translation="MSQFKKDVGDGAKAAGAAVSSSLGSGARTVVGDLGKVASVTGVA
                     VAGIGAAALKTSSDFNASMSGVASVANASSAELDQLREAALKAGADTVFSATEAAQAQ
                     SELVKAGVSVADVMGGALAGSLDLAAAGQLDLANAATISAQAMNLFGLGGADVSHIAD
                     VLAAGANKSAADVGTLGDALRQGGLVAAQTGLSLEDTVGVLSLFADSALVGSDAGTSL
                     KTMLQRLVPQSDEAAEAMKNLGLQFFDVEGAFVGIDEVAGQLQTALGGLSDEQRNAAL
                     TTLFGSDAVRAASILMEGGKAKVDEYTAAVNDMGAAQRMASTQTDNLKGDIEEFSGAV
                     ETSLIRLGDLGDVAGRQLVQSATAFVDVFNTFATTPAWEAISRNVAELSTGVGGGLTG
                     LADDLQGVLNAVSPAEVDAVFGRLEGGFTTIREAARGLEGVIAGVGLSIGGLGAASVL
                     GPLGGIVPTISPITGILGGLVLGSDAGRDALIGLGKQAAAFATGPGAGLLRSASDLAD
                     ELSEGLASVLSDVGEAAFDAAAKLGPVLGDAMEDLGPPVADILEALGELAAGVLPELA
                     ELAGTVLPPAVDVLGAGLSIAADAVGLLAENTELLVPALAAFAAIKFSDQFRGIGDVV
                     KGAAESVGSFRDAIGQIAATNNVSKLEALKGAAGATVKEVGGLAGGFIAANGPLIALT
                     AAAAGAGYIFKEFAADAGRVAEVSKDLGEAIKSTREEFAAKNEEATRSAFADLEVLDV
                     QNQLGRSLEDMSELAAKYGDNLGTLAGRYSEASDGTRSFKDLISDLPKPVQELALQIQ
                     SLEEAGDIDYSQAIRYFTALGDLAAGTADRVKAAKEEFTSIAQEAADAGKISAEELKT
                     VLAAISEAKTPDELNAAFTMLNNMLGITASAARTAADEMQRLIDMIDKLSNASRDADE
                     GARRLAESRDRVAKAFAEGGAGIDANTEAGRRNADAIRAQVEASEKLAETQALLDPTG
                     QQSTRTLLAQRQALQDLANQGLLTAEELQHLIEIYGLTDQDITATVSADTSAAETSLA
                     EVQTEFDKVKANLEVPVQTQVQALIDQGKYAEAEALLAKLERDRVVSIKARITTAVGG
                     VIAEIEKVTGTDINGNGAIGRAAGGPVWPGQLFKVGEQGPELGYFPKAGQIIDAATTE
                     RLLGTDPKALSGSEMSMAGVEQRIDTLTGEVRTLADALSAVATRPIPRPVVDVHASTG
                     VLVTAQTRARGVRSGD"
     gene            11363..11857
                     /locus_tag="PLZ72_09270"
     CDS             11363..11857
                     /locus_tag="PLZ72_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36525.1"
                     /translation="MVGLPIPEWGLVTLNDIEMDAPGRIGLNSYVLTDGAPRRRKNKV
                     RPGVDGELGSKARKTPRSVQFEVFLDGRWDADGDPTDTSDALAVIAAVQSHILYLRTA
                     ILDDDGDAEGCVDCVVTSAIPGTTHQGPVQVDDLVADPGIGAQVVTFDLTIIRGDLAI
                     VAGS"
     gene            11866..>13457
                     /locus_tag="PLZ72_09275"
     CDS             11866..>13457
                     /locus_tag="PLZ72_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36526.1"
                     /translation="MTVDLRNGANTSGSEITGAFRPSWQEQRNGLGTGSFFLPNPLAA
                     TISKGDVILGRLNGTARMAFIAERAQPQLVSVSDELMEATKWSGRGLLSEWADSKLHD
                     TPTLSNMARTVPVLDDRVMAWWGPDYDETGDGWVDSTVIAVQGWESTFYTGLPAEWSD
                     PPALWIGPSSGDDENAPAGRCLFRRWTLIGEGPAALDYAGDNTVVAYINGRRVGEGGF
                     RRKQRYEFEVTQSGWMLLAFELTNAPDDGDPGGNPTALLFSLQAADGSVYCRSDSDTK
                     ILEYPSVDPEVPLGRQIRQVMIGNPLLEGVWTVTGTETTDDQGTTFPTTGPMTYRLGS
                     DSPWDVVSQACQRHIDIRVDVEGRTLRPFVKGTVGDPSGLTLVSGYSDAGVADPDSVN
                     LDDLQWDCPRAEFDALIVRWARGRFTRGSGSRFGTLDLGQIENQAVAESIADGLLTVV
                     AEQENSASFSVLNPSEVPLPHDTLAVPGPFDVNESETLPVTGVTISGDDDGDAQVDIE
                     VGSLVEDRRTLLERATRRSDVTG"
BASE COUNT         2143 a         4591 c         4599 g         2024 t
ORIGIN      
        1 ccgccccgca cggggcacaa ccaaccaccc gcacgggagg aacccaccgt gagtaacacc
       61 cagccggatc ctgcacagga gccgacgccg aacgatcccg ccgacgcatc cgacgttccg
      121 acccctgcac agggcgacga cgatccggtc gccgaggttg cccgcctccg ttccgagctg
      181 cagaaggctc ggaagtggga ggagcgagcg aagacgaacg ctggagctgc gaaggagctc
      241 gagggtctcc gcaagcagta cgagtccgat caggaacgtg ctgtgcgtga ggctcgagaa
      301 gctgctcgca gtgaagtgct cggcgagctc ggggccgaac gtgtcgccga tgccttccga
      361 gtcgcggccg ccggtcgtgg cctggatgtc gacgaagtga tcgacgggat caacctcgcc
      421 aagttcttga gcgaggacgg caaccccgac cgcgacgccg tccaggcgtt cgtcgatcgg
      481 atcgcacctg cgcaggagac ggccccgccg gatctcggtc agggcgcacg cggtggtcag
      541 caggtaccag gcctgaacag ctcccagctg cagaaggacc tcgaagcggc gctcggcatc
      601 cgctgaccgc ctcaccccct agatccgtcc acaggaggac accatggcga tctccgcccc
      661 caccaagacc agcgacctct ccgggttcat cacgcccgcc gaggccgctc ccatcttcga
      721 gcgggccgcc cgcacctccg tggtgcagca gctctctccg caggttcccc tcggcccgtc
      781 cggggtgaag atccccgtca tctcgacccg ccccaccgcg gcgtgggtcg gtgagggtga
      841 gcagaagccg gcctccgccg gcagcatcac cccgaagacg atcgagccga agaagctcgc
      901 gtcgatcttc gtggtctccg ccgaggtcgc ccgcctcaac ccggcccagt tcgtgacccg
      961 gatgcagggc agcttcgccg agacgttcgc cgtcgcgttc gacctcgccg cgctgcacga
     1021 cgtcggtccc gacggctccg ccggcgccgg cccgttctcc acctacgtcg cccagacgac
     1081 gaagacccag gagatcggcg cgaccgccca gaacgccggc ggcgtctacg gcgacctgaa
     1141 cgcggcgctc ggcaaggtcg tcagcggcaa ggacgcctcc ggtcggcgct accgggtcac
     1201 cggctgggcg ctcgacgacg tcgtcgagcc cgccatccgt ggcgccgtcg acacggccgg
     1261 tcgccccatc tgggccgacc tcgcctccga gcaggagagc gacctcttca cctccggtcg
     1321 gctcctcggc cgccgcggct tcttcggtga gggtgtcgcc acccccgacc tgaccaccac
     1381 cgtcggcttc ggcggcgact ggggtcagac ggcgtggggt gtcgtcggcg gcatcagcta
     1441 ccggatctcc accgaggcct cggtgacgat caacggcagc ctcgtctcgc tgttcgagca
     1501 caacctcgtc gcggtcctgg ccgaggcgga gtacgggtgg ctcgtcaacg acaccgcggc
     1561 gttcgtcgag ctgacgaaca ccaacaacag cccgatcacc tcgnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnntacccgc cgtcgtcgcg ggtcggggcg tggctctcca
     1741 cccacgagtg ccttcaacgc ctcgccgaac ggggccatga agttcacgtc cgcaccgtgt
     1801 tcgccccagc ggactacacg cacggtgacg tgcgggtgct cggccgatgg gaccgctcag
     1861 tcgccggcta cgacgccgtc gtctcacacc acggcgacac cacctccggc gtccgacagc
     1921 tctgcatcaa gcgtgacatc ccgcacgtcc tgatggtgca cggaaccccg gatcaggtgg
     1981 acgacagcga ggggccggtc gtgtggaaca gcgagtcatc ccgggctggc cgtaacggca
     2041 tcgtggtccg cccgcacgtc gaccccgcca tctaccggac caccgccggc gaccacgtca
     2101 cgctcatcaa cctctcgcga gagaagggtg gcgacctgtt tcgccagatc gtcgcagcca
     2161 tgccggaacg gaagttcctc ggcgtgcgcg gcggctacgg ccgccaacgc ataccggccg
     2221 acccgaatgt cgagatggtc ccgacaacca ccgacatggc agcagacgtc tacagccgga
     2281 cccgggtgct cctcatgccg tcgatcgccg agacgtgggg tcggaccggc gtcgaagcta
     2341 tgtgctccgg gatccccgtc atcgcacacc cgacacctgg cctggtcgag tcgctcggcg
     2401 acgccggcat cttcgtcgac cggaacgacc tcgccggctg ggtcggcgct atccgacgcc
     2461 tggacgaccc cgacgagtac gcccgccggt ccgctgcggc gctcgccagg gccgccgaac
     2521 tggacaccgc tacggacctc gaccggttcg ccgactacgt ggaggcctgt gcgtgatcgc
     2581 cgtcctcatc cccaccttcg ggcgtgccga ccggctcgcc cgggtggcgg ccaacatcac
     2641 cgagaacacc gagaccgatc accgggtcgt gttcgcggtc gaggccgagg acgtcgagtc
     2701 gatcgcagcc gccgcggagc tcgacgtcga cgtcgtcgtg aacgagggcc gacccaacta
     2761 ctcgggagcg atcacgacgg cgtaccgggc cactccggcc gagtacgtgt tcgccggcgc
     2821 cgacgacctg aacttccatc ccgggtggga caccgccgcg ctggccctca tggacggctg
     2881 ggtgcaggtg gtcggcacga acgacctgct gaacccgtac gtgaccgccg gcctgcacgc
     2941 cacccactgc ctcgtcgacc gccgctacct cgacgagacc ggcggtgttg tcgaccaagg
     3001 gcccggatcg ttcatgttcg acggctacga ccaccagtac accgacaccg agttcatcgg
     3061 caccgccaag atgcgagcca ggttccgtcc gtgcttcgac tctgtggtcg agcacctgaa
     3121 cgcctggtca ccgaagggtg aggtcgacgc gacggcccgc aagacgatgc gggccatcga
     3181 caccgactcg gagctctacg actcccggag ggacctgtgg ttctcgatca gcaggtgacc
     3241 ggccggcatg tcgtcgtgct cggcggcggc ggcttcatcg gagcgaacct gtgcaaccgg
     3301 ttgtacgcgc tcggcgcgca cgtgaccgcg gtcgacaccg tgtttccgtc gtggcgtcgg
     3361 cctgacgtga tgtgccgccg agccgacctg acgtcgctcg ccgagacggt ccgcgcgatc
     3421 gtcggcgccg agctcgtgtt ccatctggcg gccgacatgg gcggcgtcgg ctacttccac
     3481 agcaacgccg atctgggcgc ctcgctcgtc aacggtcaga tcaccctcaa cgtcgcccag
     3541 gccgtcgaag cgcacgagac accgaccacg ttctacgcgt cgtcggcgtg cgcgtacccg
     3601 gtcgagctgc agcagacccc gggggaagcg ccggcgctct ccgaggacct gatcggtcac
     3661 ggcacgcccg acgccctgta cggggcggag aagctccacg ggctgcggat catgggcaag
     3721 gtgcccggcg cccgggtcgg tgtcctgcac acggtgttcg ggccgctgca ggagcacgag
     3781 ggacggcgca tgaagttccc cgccgcggtg gcgacgaagg cgctcgccgc acggtcgtcc
     3841 ggcacgctcg agctgtgggg cgacgggtcg cagctccgct cgtacctgca cgtggacgac
     3901 gcggtcgatc ggatcatcac gatcgcgacc gcaggccgct acgacggccc ggtgaacgtg
     3961 ggggccgcgg gcccggtcac gtgccgggag atcgccgagc tgtgcctgga catcgtcggg
     4021 gctgacgctg acatcgtgac gaacccggcg gagccgaccg gtgtgctcgc tcgggactgc
     4081 tcgaacgccc gctacgacga cctgtacggg cccgggccgc agcggtccta ccgggccggg
     4141 ttcgagtcct tcatctcctg gctggaccgg ctgtgagggt gcacgtcggc atcccgtggc
     4201 gggccggtga cccgtggcgc gagcaggcgt tcgcttacgt ccagatccac ctacgcggcg
     4261 ccggcctgga cccggtcccg tacgacaacg gcgccgacat cttctcccgg gccgggtccc
     4321 gcaacctcgc cgtccgctgc gctgacgccg acgtcgtcat cctccacgac gccgacatgg
     4381 tcctgcccgc cgctgcgtac ctcgaggcgg ccgagctcgc cctgtcgtcg gaccggctgg
     4441 tcatcgggtt caaccggtac cggccgctgt cgaaggcgcg cacggtcgag gcgttcggcg
     4501 gcgccgaccc gttcgcgctc gagcccatcg acgagctcgt cgacttctcg gtcggcgggg
     4561 tgctcgccat cacgccggag tgctggtggg cggctggcgg catggacgag cggtacgtcg
     4621 actggggctg tgaggacttc gcgttcgccg acgcggccgg cgcagcgctc ggcccggtgc
     4681 tccgtgtcga cgggcccgcg gtccacctgt ggcacccgca cgccggcgac ccgacgaacc
     4741 ctgatcagca ggccaactct cgactgctcg caggaggtga acgatgacga cgaggctcgc
     4801 caccccggat gatctcactc gccgcatggg catcaccgtc gagagcgagc gcgcactcgc
     4861 gctgctcgcc gatgcgtccg ccgccgtcat ctcgtacacg ggccagcagt tcgtgcgggc
     4921 gacgaccacc gcccaagtgc gggtgccgtc ggaccggaag gttcggctcg ggcagctccc
     4981 cgttcacgac gtcgacgccg tcacggacct gaacgacaac cagctcctgt tcaccttcga
     5041 cggcatcgac accgtgcacg tcacggtgaa cctcgactcg ttcagcttcg agccgtggac
     5101 gagcccgatc cgcaccgtca acgtcaccta cgaccacggg ttcgacgcca tccccgacga
     5161 tattgtcgcc gtcgtctgcc aggtcgcggg cagggcctac gggaccgccc cacaggacac
     5221 gggcgccgca cgcgaacagc tcggcgactg gagctactcg gccggtgccg cggcctcatc
     5281 cggtgcgctc ggcctattgc tgccggaacg ggcagtcctt gaccggtaca cccagcgggt
     5341 ccggacgatc ggcatcggac cgtgaggacg cccgagcacc tgatgacccg cctcggcaag
     5401 atcgtcacgg tcgccgacgg cgacgcgacg gacgtgtacg gcaacgtcga accgaccgag
     5461 gtggagacca cggtgcgcta cgagctgcag cagcagcgtc gcgacgaggc cggcgacccc
     5521 gacaactggc agatcggggc atggagcctc tatctgccgt cagggaccgc cgtcactggc
     5581 tgggatcgct tcatcgacga ccagaacgac gagtacgaac tggtcgggcc gccggagcgc
     5641 aagtggaacc cgcgggctca gcagttctcc cacgtgcagg ccacggtgcg gagggtgacg
     5701 tgagccgctt cgtgcccgca gcgaaccttg acgtcgtcct cgatcacgcc acgcacgacg
     5761 atcgactcaa ggtcgccgag cacatggcga tcgacgcacg ccacaacgtg aacccggaca
     5821 ccgggcacta cgccgaagag gtccagacgt tcgacgacga ccgaggcgtc ggcgtcgatg
     5881 cccacgacgt cgccgcgcac atcatcgagt tcgggtcaat caacaacgcg ccgcaggcgc
     5941 cgatccgtcg tgccgccgcc gagcacggga agttcgaacc ccgatgagtc tcgtcctgtt
     6001 gccgaacacc gcagcgctca cctcggcgtt cctgcgtgcc caggacgaag tggcggacct
     6061 cgtcgacgac cgggtctaca cgatcctgcc gaacagcaag acgttcccgc ttgtgcgggt
     6121 gcgccgcatc ggcggcaccg ccggcacacg gttcgcccac tggctcgacg ccccactgtt
     6181 ccaggtcgac gcctgggcag acaccgccgc cgtggcgttc gaagtcaccg aaacctgccg
     6241 tgcagcgctc gtccagcgct tctccggcat ctatcacttc accggtgtct ccggtgtcgt
     6301 gacgtctgtg gaagcaggcg gaatcaccga agggttcgac cctgacgagc agacgaaggc
     6361 tcgcggccgc ttcgacgcgg tggtgatggc gcatccggcg cccgacctga cttccagcta
     6421 gatcacccgc cggcctccgg tcggagcgtg aaaaccatca gtcccgccga agcccggcgg
     6481 ggctttgccg ttcaaggagg ccccaaaatg gcagtcgaaa ccccggaagc gatcctcatc
     6541 gcagcaggat tcgagatcta caccgcaccc gtcgggacgc cgatcccgac cgatgtggac
     6601 tcgactctca acgctgcgtt cacgcgagtc ggctacacca ccgaagacgg tgctgtgttc
     6661 acccgcactc cgtcggtgac gccgatcaac atgcaccagt cgttcgaacc ggtgaggtac
     6721 gtcctcgccg gcatcgagac cacgctggcc ttcaccctcg gccagtggga cgccgacacg
     6781 atccccctcg ccttcggtgg tggtacgtgg tcgaccaccg cgaacgggtg gcggttcgac
     6841 ccggccgagc cgggcgtgat cgacgagcgc gtcgtcgtcg ccgacgccgt cgacggtgag
     6901 accaagtggc gcatcgtgat gcgtcgcgtc atgcagcagg gagcgtcgtc ttcgacgctc
     6961 acgaagtccg gcgccgctgg actcgccgtc accctcggct ccatggccgc cggctcgggc
     7021 accaagccgt ggtcgatgtt caccgacagc gagtacgtca acccggtcac ctcggcctga
     7081 tgcccgacac ccccaggacc atcgactact cgaccctgcg actcgcggtc gagtacgccg
     7141 acggcacgcg cgtcgagggg atccgtgtga tcccctcggc gactcgtgcc atcgaagccg
     7201 agttcaacga gcagttctcg aaggtcgcca cgttcgaccg cgaggactgg atgcacaccg
     7261 ccgcgtggac ctacctgaag ctgcgcgagg gcgaggaccg gccgttcgac gagtggctcg
     7321 ccaccgtcga ccgactgcag atcggggtgg cggatgacga ccgccctacc tcgggcccga
     7381 ctcgcaagtc gtccggctca tccgggcggc gctcgacgaa ggccaagacc tcaacggact
     7441 agccctcctc ggcgaccggg accccggcct cttcgaggcc ttcctgttga tcgccgacga
     7501 gcgacgggcg cggcgggaac tccccgccgc tgctgtcgct gcccctcctc ctgttcctgc
     7561 gccgccgccg gcccgttcgg tggctgcatt cccgcctgac ggtctcactc cggcaacgcc
     7621 ggacgagatc cgggccttct tcgcgaagag gtgaccgcat ggcgcaggcc ggaacagtgt
     7681 gggtggacgt ccgcggcgac atgtcgcagt tcaagaagga cgtcggtgac ggagcgaagg
     7741 ccgccggggc agcggtcagc tcctctctcg gttccggggc ccgcaccgtc gtcggtgatc
     7801 tcgggaaggt cgcttccgtc accggcgtcg ccgtcgctgg catcggcgcc gcggcgctca
     7861 agacgagctc cgacttcaac gcgtcgatgt ccggggtcgc gtcagtagcg aacgcgtcat
     7921 ccgccgagct cgaccagctg cgtgaggccg cgctgaaggc cggtgctgac acggtgttct
     7981 ccgcgacgga ggcggctcag gctcagagcg agttggtcaa ggccggtgtg tcggtcgcgg
     8041 atgtgatggg cggcgctctc gccgggtcgt tggacctcgc cgccgccggc cagctcgacc
     8101 tggcgaacgc ggccacgatc tccgcgcagg ccatgaacct gttcggtctc ggcggcgcgg
     8161 acgtgtccca catcgcggat gtgctcgccg caggggcgaa caagtcggca gcggacgtcg
     8221 gcacactggg cgatgcgctc cgccagggcg gcctcgtggc cgcacagacg ggactctccc
     8281 ttgaggacac cgtcggggtt ctgtcgttgt tcgccgactc ggcgctcgtc ggttccgacg
     8341 ccggcacgtc gctcaagacg atgctgcagc ggctggtgcc gcagtcggac gaagcggctg
     8401 aggcgatgaa gaacctgggc ctccagttct tcgacgtcga gggcgcattc gtcggcatcg
     8461 acgaggtcgc aggtcagctg cagacggcgc tcggtggact gtccgacgag cagcggaacg
     8521 cggcgctcac cactctgttc ggctccgatg ccgtgcgggc ggcgtcgatc ctcatggagg
     8581 gcggcaaggc caaggtcgac gagtacacgg ctgcggtcaa cgacatgggt gcggcgcagc
     8641 ggatggcgtc aacccagacc gacaacctga agggtgacat cgaggagttc tcgggtgcgg
     8701 tcgagacgtc gctgatccgt ctcggtgacc tcggtgacgt cgcaggccgt cagctggtgc
     8761 agtccgcgac ggcgttcgtc gacgtgttca acacgttcgc caccacaccg gcgtgggagg
     8821 cgatctcccg caacgtcgcc gagttgtcga ccggtgttgg cggtggactc accggccttg
     8881 cagacgacct ccagggcgtc ctgaacgctg tgtcgccagc tgaggtcgat gctgtgttcg
     8941 gtcgcctcga aggcggcttc acgacgatcc gtgaagccgc ccgcggactc gaaggcgtga
     9001 tcgccggcgt cggcctgtcg atcggcgggc tcggtgcagc atcggtgctc ggtccgctcg
     9061 gcgggatcgt tccgacgatc tcgccgatca ccgggatcct cggtggactc gtgctcggat
     9121 cagacgccgg ccgcgacgcg ctgatcggtc tcgggaagca ggcagcggcc tttgcgaccg
     9181 gcccgggggc cggtctgctg cggtccgcgt cggatctcgc cgacgagctg tccgaaggcc
     9241 tggcgtcggt gctgtctgac gtaggcgagg cggcgttcga cgctgccgcg aagctggggc
     9301 ctgtccttgg cgacgccatg gaggatctcg gcccgccagt cgcagacatt ctcgaggcgc
     9361 tcggcgagct cgctgcaggc gtgctgcctg agttggcgga gctggccggc acggtcctgc
     9421 cgccggcggt tgacgtgctc ggcgctggcc tgtcgatcgc cgcggatgcc gtcgggttgc
     9481 tggcggagaa cactgagctc ctggtccccg ccctggcggc gttcgccgcg atcaagttct
     9541 ccgaccagtt ccgtggcatc ggcgacgtgg tcaagggtgc cgctgagagc gtcggatctt
     9601 tccgcgacgc gatcggtcag atcgcagcga cgaacaacgt gtcgaagctg gaggcgttga
     9661 agggggctgc gggagccacc gtgaaggagg tcggtggact ggcgggcggg ttcattgccg
     9721 ccaacgggcc actgatcgcc ctgaccgcag cggccgcagg tgcggggtac atcttcaagg
     9781 agttcgccgc cgacgccggc cgcgtggccg aggtgtccaa ggatctcggc gaggcgatca
     9841 agagcacccg cgaggagttc gccgccaaga acgaggaggc gacccggtca gctttcgccg
     9901 accttgaggt gctcgacgtt cagaaccagc tcggtcgcag cctcgaggac atgtccgagt
     9961 tagccgccaa gtacggcgac aacctcggaa ccctggctgg ccggtactcc gaggcatccg
    10021 acggcacgcg gtcgttcaag gacctgatct cggacctgcc gaaacctgtg caggaactgg
    10081 cgttgcagat ccagtcgctc gaagaggcgg gagatatcga ctactcgcag gcgatccgct
    10141 acttcacggc gctgggcgac ctggccgctg gcacagccga ccgagtcaag gctgccaaag
    10201 aggagttcac gtcgatcgcc caggaggcgg ccgacgccgg gaagatctcg gccgaggagc
    10261 tcaagacggt gctggctgcc atctcggaag ccaagacgcc cgacgagctc aacgcagcgt
    10321 tcacgatgct gaacaacatg ctgggcatca cggccagtgc cgccaggacg gcggccgacg
    10381 agatgcagcg gctgatcgac atgatcgaca agctgtcgaa cgcctcccgg gacgcggacg
    10441 agggtgctcg ccggctggcc gagtcccgcg accgggtggc gaaagcgttc gctgagggcg
    10501 gtgcgggtat cgacgcgaac actgaggccg gacgccggaa cgctgacgcg atcagggcgc
    10561 aggtcgaggc gtcggagaag ctggccgaga cgcaggctct cctcgatccg accggccagc
    10621 agtcgacgcg cacgcttctc gctcagcgtc aggcgcttca ggatctcgcc aaccagggtc
    10681 tgctgaccgc cgaggagttg cagcacctga tcgagatcta cggactcacc gaccaggaca
    10741 tcaccgcaac cgtgtcggcc gatacgtcgg cggcggagac gtcgctcgct gaggtgcaga
    10801 ccgagttcga caaggtgaaa gcgaacctcg aggtcccggt ccagacgcag gtgcaggccc
    10861 tcatcgacca gggcaagtac gccgaggccg aggcgcttct cgcgaagctc gagcgtgacc
    10921 gtgtcgtgtc gatcaaggcg aggatcacga cggccgtcgg aggggtcatc gctgagatcg
    10981 agaaggtgac cgggaccgac atcaacggca acggtgcgat cggccgcgcg gcgggtggcc
    11041 cggtgtggcc cggtcagttg ttcaaggtcg gcgagcaggg cccagagctg ggctacttcc
    11101 cgaaggccgg tcagatcatc gacgcggcga cgacagagcg gctgctgggt accgacccga
    11161 aggcactgag cgggtcggag atgtccatgg cgggcgtcga gcagcgcatc gacacgctga
    11221 ctggcgaggt tcgcactctg gccgatgcgt tgtctgcggt ggcgactcgg ccgattccgc
    11281 gtccggtggt cgacgtccat gcaagtaccg gcgtgctcgt cacggcacag acccgtgcgc
    11341 gtggtgtcag gagtggtgac tgatggtcgg tctcccgatc cccgaatggg gactcgtcac
    11401 gttgaacgac atcgagatgg acgcccctgg ccggatcggg ttgaacagct acgtcctgac
    11461 cgacggggca ccgcgccgcc ggaagaacaa ggtgcgtccc ggcgtcgacg gcgagctcgg
    11521 atcgaaggcc cgcaagacgc cccggtcggt ccagttcgag gtgttcctcg acggccgatg
    11581 ggacgccgac ggagacccga ccgacacctc ggatgcactg gccgtgatcg cggcggtgca
    11641 gtcgcacatc ctctacctgc ggacagcgat cctcgacgac gacggcgacg ccgaggggtg
    11701 cgtcgactgc gtggtgacct cggcgatccc gggcacgacg catcaaggcc cggtgcaggt
    11761 cgatgacctt gtcgctgatc ccggcatcgg ggcccaggtc gtgaccttcg acctgacgat
    11821 catccgtggc gacctcgcga tcgtggcggg gtcctgatgg tcgaggtgac cgtcgatctc
    11881 cgcaacgggg cgaacacgtc ggggtcggag atcaccgggg cgttccggcc gtcgtggcag
    11941 gagcagcgca acgggctcgg gaccgggtcg ttcttcctgc cgaacccgtt ggcggcgacg
    12001 atctcgaagg gcgacgtcat cctcggccgc ctgaacggga ccgcccgcat ggccttcatc
    12061 gcggagcggg cgcagccgca gctggtgtcg gtgtccgacg agctgatgga agcgacgaag
    12121 tggtcgggcc gtgggctcct gtcggagtgg gccgactcca agctgcacga caccccgacg
    12181 ctgtcgaaca tggcgcggac ggtgccggtg ctcgacgacc gggtcatggc gtggtggggg
    12241 ccggactacg acgagaccgg cgacggctgg gtcgactcga cagtcatcgc ggtgcagggc
    12301 tgggagtcga cgttctacac gggcctgccc gcggagtggt cggatccgcc ggcgttgtgg
    12361 atcggtccgt cgtccggtga cgacgagaac gcaccagcgg gccggtgtct gttccgccgg
    12421 tggacgctca tcggtgaggg cccggcggcg ctcgactacg ccggcgacaa cacggtcgtc
    12481 gcgtacatca acggccgtcg cgtcggtgag ggcggcttcc gacggaagca gcggtacgag
    12541 ttcgaggtca cccagtccgg gtggatgctc ctggcgttcg agctgacgaa cgcacccgac
    12601 gacggcgacc cgggcggcaa cccgaccgcc ctgctgttct ccctccaggc cgcggacggg
    12661 tcggtgtact gccggtccga cagcgacacg aagatcctcg agtacccgtc ggtcgatccg
    12721 gaggtgccgc tcggccggca gatccgtcag gtgatgatcg gcaacccgct cctcgaaggt
    12781 gtgtggacgg tgacggggac ggagacgacc gacgatcagg ggacgacgtt cccgacgacg
    12841 gggccgatga cgtaccggct cgggtcggac tcgccgtggg atgtggtgtc gcaggcgtgc
    12901 cagcggcaca tcgacatccg agtcgacgtc gaggggcgga cgctgcgccc gttcgtgaag
    12961 ggcaccgtcg gcgacccgtc cgggctgacg ctcgtgtccg gctactccga cgcgggagtc
    13021 gccgacccgg actcggtgaa cctcgacgac ctgcagtggg actgtcctcg cgccgagttc
    13081 gatgcgctca tcgtccgctg ggcgcgtggc cggttcacga ggggttccgg gtcacggttc
    13141 gggacgctcg acctcggtca gatcgagaac caggcggtcg ccgagtcgat cgcagacggc
    13201 ctgttgacgg tggtggcgga gcaggagaac tcggcgtcgt tctcggtgct gaacccgtcc
    13261 gaggtgccgt tgccgcacga cacgttggcg gttcctggcc cgttcgacgt gaacgagtcg
    13321 gagacgctgc cggtgaccgg tgtgacgatc tccggcgacg atgatggcga cgcccaggtg
    13381 gacatcgagg tcgggtcgct ggtcgaggat cgtcgcactc tgctggagcg tgcgacgcgc
    13441 cgcagtgacg tgacggg
//
LOCUS       DAOQOD010000082        13363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1636_length_13363_cov_11.129847, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000082 DAOQOD010000000
VERSION     DAOQOD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_13363_cov_11.129847"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..969)
                     /locus_tag="PLZ72_09280"
     CDS             complement(163..969)
                     /locus_tag="PLZ72_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNA36527.1"
                     /translation="MDDDPADGHDGSDGDDSDLFEYDATLDPADDAVPDVLRAGDIEV
                     LGAMPWSSNNTFLVQVTCGAEHLPAIYKPERGERPLWDFPDGLWKREVATAVLSAHLG
                     VGLVPPTVTRGDEDGRGAPAPAGRGSLQAFVPARFAEHYFTLRDRDDLADTFRTLAAF
                     DLVVNSADRKSGHCLIDGRDRIWAIDNGLTLHREFKVRTVIWDFAGDAVPDPMLRALD
                     DMLGRPLPAELAELLDPFERDALRDRAAGVLAAGRLPHDPTGRRVPWPLV"
     gene            complement(976..1521)
                     /locus_tag="PLZ72_09285"
     CDS             complement(976..1521)
                     /locus_tag="PLZ72_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HNA36528.1"
                     /translation="MTSRSFEFDHLDALIPGTVGEPGARIFYLQARRGNEVVSFRLEK
                     EQVGMLGQYLGQLAQALGSTEAERDVTGLIEPVEPEWVIGSLSVGVDEDAGLIFVNAE
                     ELVPDDSDPFEDLEDFDDDTVDELLAHEPEGAEARFTLQLGQAVAFAEAAMDLVRAGR
                     PPCPLCGRPIGTEGHACPRWN"
     gene            complement(1518..2228)
                     /locus_tag="PLZ72_09290"
     CDS             complement(1518..2228)
                     /locus_tag="PLZ72_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA36529.1"
                     /translation="MADGSSTARTRSGPARRAAPRTRKTPRPTTVLLVRHGQTSTTGK
                     VLPGRAPGLHLADAGVAQAERAAARLGALDVAAVYASPLERTRQTAAPIGRATGRRVR
                     TAKGLLECDFGTWTGQSLARLRKLPEWSTVQTAPSRFRFPGGESFAEMQLRIWNEIER
                     LVALHPGDTVVAVSHADPIKAAVATAAGVHLDLFQRTVISPCSITALLMPAAGSPGTP
                     IVLTVNSTGDDLSGLAPS"
     gene            2253..2939
                     /locus_tag="PLZ72_09295"
     CDS             2253..2939
                     /locus_tag="PLZ72_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNA36530.1"
                     /translation="MEPLQALAAEIVECRRCPRLVEWREQVAREKRAAFRDQEYWGRP
                     VPGFGDPDARLLIVGLAPAAHGANRTGRMFTGDRSGDFLFRSLWRAGYANQPTAVSRD
                     DGLELTDVFITAPVKCAPPQNKPTTVERDNCRPWIDAEVDLLRGLRVVVALGGFGFAE
                     ALRIFGDGSMRPRPRFGHGVEVGLPGGRTLVGCYHVSQQNTFTGRLTEEMLDAVFART
                     RELCAQEPTA"
     gene            3088..3390
                     /locus_tag="PLZ72_09300"
     CDS             3088..3390
                     /locus_tag="PLZ72_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36531.1"
                     /translation="MNVAPTTVAEIVPFAEYGEDVLADNVFSGPADATPAWLEGHLGR
                     VTQLPRGRKGLWGALATNVVPPRVGLSAPVDAGGLAPVRRPMFDVEPGLVIDLDVA"
     gene            3549..3905
                     /locus_tag="PLZ72_09305"
     CDS             3549..3905
                     /locus_tag="PLZ72_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36532.1"
                     /translation="MRPRSPRSILLGVALASLLALSACGQRTQPTGYGEDYQKNFMLG
                     CNEQTAVPEENPEVTTPGPEAPKNFCECVYDGLVEKVPFDDAKKFEEQQAKEDAGDIA
                     VPKPIQKVIDSCNKES"
     gene            complement(3958..5199)
                     /locus_tag="PLZ72_09310"
     CDS             complement(3958..5199)
                     /locus_tag="PLZ72_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNA36533.1"
                     /translation="MADGDDTARSTTTTTDPVADLEQRLADNAATRWTNQWAELKAEV
                     VDTGLCTGCAACVIACPHDVLGYDHESGGYHPYHLEDELGPDDCIHGQKGCTACTRAC
                     ARFRLWEGQADDHLFGRLRRPDEVAGIYHDVLLTRASDDAVNAAGQDGGLVSAILLWA
                     LEHDYIDAALVSYLEAGEGGAGSSWRPKPGVASTPEQILAAAGSRYTYSANTLALDEA
                     IEAGFSRLALVGMSCQSSAPPVMWSRKVGKISKPIVFNLGLLCSKTFDDAIFEELFWA
                     KYGLARQHMVKMNIKGVFQIWMDDGAYHEINLKECHAWTREGCMYCPDFAAEHADISC
                     GGIGENSDWTLTIVRTDLGREIISRMIDQGVIESKPGDSDPGAIALMRKLAEKSRARW
                     PTSAEPAVRVGLPDPTVRSKG"
     gene            5356..6600
                     /locus_tag="PLZ72_09315"
     CDS             5356..6600
                     /locus_tag="PLZ72_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36534.1"
                     /translation="MDLTYPPEAEEFRTEIRAWLEENLPDGWFDEGFEMTPEQKQAFN
                     DDWTAKLFEGGWICATWPAEYGGKGLTTMQGVVLAEEFAKAKAPMRADFFGDTLVGPT
                     ILMNGTEEQKKHFLPRILDGSMRWCQGFSEPDSGSDLASLKTTAVQDGDEWVINGQKV
                     WTTQAQFADYCFVLARTDPDVKKQAGISYLLVPMDQPGIEVRGIVQPDGTAEFNEVFF
                     TDARCPIDNVVGGVNNGWKVANDTLGFERGMSATTGYRRFAEEYRLMVEKAQSNGTID
                     DPTIRQRLMEYYTKIQIMRINGLRSLTATVTGKRDMGVAALGVTNKMFWSETHKAAME
                     LALDIWGAEAMLSTAAPDSGSWPATMRADGREAYPVSPMMSSFFFSRSETIWGGTSQI
                     QRNIVGEKLLGLPREPKPAAAG"
     gene            6898..8313
                     /locus_tag="PLZ72_09320"
     CDS             6898..8313
                     /locus_tag="PLZ72_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNA36535.1"
                     /translation="MLLGIVSVLGVLALIAGAGAAWGLWSFNQFGRKDLDLAHAEDDA
                     PRNFLVIGSDSRAGVRKGDKGSGVMLGKGTPGGQRSDSIAIMRIDPGEERIDMLSIPR
                     DLWVPIAGTGKEQRINTAYAESTQTLIDTIQSDLGIPINHSVEVDLVGFQKLIDAIGG
                     VPMYFPNAVKDRNSGLVVQKKGCMTLDGYMGLAFARSRHLEWSDGVKWHTDPTGDLGR
                     MTRQQLLVRTALGQVKHLGLDDIGSLKGIVDAAVGSVTLDSNMGTSDIIDLAQRFSDF
                     DPAKLQTHSLPVTGHTTDGGASVVLLDQAAAQPVLDIFRGTSASAVTTTTEPPPSPGD
                     ITVDVLNGTTRQGEARRVSFVLTDGGFGTGTVDSAPDPTEATVVHYPKGGRAMAELVA
                     TWLGPAPKLKEDSKLAPGRVTVTIGDDFDSVSEPAGAPAPTDPTPVDPAVEAGGSAPS
                     TTTTTEPGWTPGAVPDGVRCG"
     gene            8357..9316
                     /locus_tag="PLZ72_09325"
     CDS             8357..9316
                     /locus_tag="PLZ72_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNA36536.1"
                     /translation="MVDPAARRSRRPVPRAGTVLVAILAVAAIAVGLGSCSGDDSASG
                     SPEGECPVAPVSVVATIGPWGSIAEELAGDCAHVTTIVDGSRGDPHDVEPTPADRAAI
                     EDADVVITNGLGYDAWADDALAAASSEPAVVVAGEVTGQETGANPHAWYDPAIVREAA
                     TAITQALQEASPTEAEEYYTSRFQTWEQSLAPYDAAIERVRAGAAGRPYAATEPVFDP
                     MAAALGLLDVTPSGFSQSVSNGAEPSPGDVAAFDAVLSGGKAAALIDNSQTDSAMARQ
                     LTARAAGSGVPVVEVTETAPASATSFVAWQVAQLDALAAALGL"
     gene            9394..10167
                     /locus_tag="PLZ72_09330"
     CDS             9394..10167
                     /locus_tag="PLZ72_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36537.1"
                     /translation="MSLREVEVHRGDHLVWSRGSFDIAAGSVVGVIGPNGSGKSTLLR
                     LLLGLLEPTAGRVEVFGDPARRGNRRIGYVPQDDTAAQPESIRCRDLVALGAGGHRVG
                     LGPFGAARRAMAARVDAALAETASSAFAGRRLSEVSGGQRRRVSLAQALVDDPELLLL
                     DEPLANLDLRHQRETVELIERVAHRPRADGRVTTTLIVTHDINPLLPVLTGAVYLLDG
                     HPHHADIGVVVDEHLLSHLYGTSVAVVRTPQGDLFTRSV"
     gene            10169..11050
                     /locus_tag="PLZ72_09335"
     CDS             10169..11050
                     /locus_tag="PLZ72_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNA36538.1"
                     /translation="MAGRVLASGASVLADIGYQPEWTTVMGSSFMRHALIAGTLVALV
                     AGPIGYFVVVRRDSFAAHALAHIGFPGATAAVLLGAPVTAGLAVFCTAGGLAIGLLGR
                     RTVDRETATGTILALSTALGILFASMASEGAAAVTDVLFGNLLAVTTRQLWTFSIMAA
                     VVLVVLAVIARPLLFASIAPDVAAAKGVATGALGLVLMVLLALVTTMAVQVVGTLLLF
                     ALVVTPAAAALAVTARPAAAAGVSSAVALVSVWGGLVLSAVFNLPPSFPIVAIAFVVW
                     ALATAWSRTGARRPTGA"
     gene            complement(11075..11497)
                     /locus_tag="PLZ72_09340"
     CDS             complement(11075..11497)
                     /locus_tag="PLZ72_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA36539.1"
                     /translation="MDAFDADVLIYAAAPRHPLGRRVRLLFPDDAAPAGGAVAGIGSV
                     LLVPELLSKPLRDGADEEVDALAAMLGRLDLRPVDEATADLAAVLGATHRLRAVDATH
                     LATAVIAGADRFITNNSSDFPTSIPEIDITYPQTLPAR"
     gene            complement(11508..11798)
                     /locus_tag="PLZ72_09345"
     CDS             complement(11508..11798)
                     /locus_tag="PLZ72_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNA36540.1"
                     /translation="MTTTSVSEARATLPELLDRVAAGEEITITRHGHEVAVLVRPDAL
                     LVRRADAAIADAERLRTLLQRGRTTPLSDVPALDPERADELAAAVTASRSRR"
     gene            11924..12193
                     /locus_tag="PLZ72_09350"
     CDS             11924..12193
                     /locus_tag="PLZ72_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNA36541.1"
                     /translation="MQRTNIYLAERQTEALDRIAADEGISRAEVIRRLLDRALAGGDD
                     DVRNDLAAIDSSFGLLANLGADELELDRDDGDRGRHLSQVWSTTA"
     gene            12190..12579
                     /locus_tag="PLZ72_09355"
     CDS             12190..12579
                     /locus_tag="PLZ72_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA36542.1"
                     /translation="MILVDSDILIAHLRGVPDARSWLREARLRSPLSISVVTVAELTG
                     GMRSAERREVWALLNVMRPEPVTELVARRAGELRRRFRRSHTGIGIADYLVAATALEL
                     GAELATLNVTHFPMFDDLRPPFEVPLR"
     gene            complement(12515..13057)
                     /locus_tag="PLZ72_09360"
     CDS             complement(12515..13057)
                     /locus_tag="PLZ72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNA36543.1"
                     /translation="MILVDTSAWVEFLRDTGSPACERVDALLDDDIAITDAIRMELLA
                     GARDEDHLRRLRGLTARTTVLATTSADYELAALLYLRCRHSGETVRKLIDCLIAAVAI
                     RHDVAVLHHDRDFEVLARHTELRVEAPEGGSGPDVGDEGPGIAGGARRRGAHGPVEHL
                     SAAEPRTGGGGRRTSGSASR"
     gene            complement(13054..13257)
                     /locus_tag="PLZ72_09365"
     CDS             complement(13054..13257)
                     /locus_tag="PLZ72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNA36544.1"
                     /translation="MSRTNIDLDDDACAEVMRRYHLATKRDAVNLALRLAAADALSTE
                     QARDLRGSGWDAELEALRTTRVP"
BASE COUNT         1858 a         4724 c         4835 g         1946 t
ORIGIN      
        1 accacgccgt cccagtcgcc ccgatcggcg cggcggtcca cctctcgcag cagctcgtcg
       61 aggtcgccgg tggtgatcca gcggtcgagc gccgggtcgt cgcggggttc ggggtcgggc
      121 aacgtcgatg cggtcaccgc ggtcgggctc ctcggtccgt cgtcacacca gcggccacgg
      181 cacgcggcgg ccggtcgggt cgtgggggag tcgaccggcg gccagcaccc cggccgcccg
      241 gtcgcgcagg gcgtcgcgct cgaacgggtc gagaagctcg gcgagctccg cgggcagcgg
      301 ccggcccaac atgtcgtcga gcgccctcag catcgggtcc ggaacggcgt cgccggcgaa
      361 gtcccagatc accgtgcgga ccttgaactc gcggtgcagg gtcaggccgt tgtcgatggc
      421 ccagatgcgg tcccgtccgt cgatcaggca gtggccgctc ttgcggtccg ccgagttgac
      481 gaccaggtcg aacgcggcga gcgtgcggaa cgtgtcggcc agatcgtcgc ggtcgcgcag
      541 cgtgaagtag tgctcggcga accgggccgg cacgaacgcc tgcagcgacc cgcgaccggc
      601 cggcgccgga gccccacggc cgtcctcgtc gccgcgggtg acggtgggtg ggacgaggcc
      661 cacgccgagg tgggccgaca ggaccgcggt ggcgacctcg cgcttccaga gcccgtcggg
      721 gaagtcccac agcggtcgct cgccgcgctc gggcttgtag atcgccggca ggtgctcggc
      781 gccgcaggtg acctgcacca ggaacgtgtt gttgctcgac cacggcatgg cgccgagcac
      841 ctcgatgtcc ccggcgcgca gcacgtcggg gacggcgtcg tcggccggat ccagcgtggc
      901 gtcgtactcg aagaggtcgg agtcgtcgcc gtcgctgccg tcgtgcccgt ccgcggggtc
      961 gtcgtccacg tcgggtcagt tccagcgggg gcaggcgtgg ccctcggtgc cgatcgggcg
     1021 accgcacaag ggacacggcg ggcgtccggc ccggaccaga tccatggcgg cctcggcgaa
     1081 cgcgaccgcc tggccgagct gcagggtgaa gcgggcctcg gccccctccg gttcgtgggc
     1141 gagcagctcg tcgacggtgt cgtcgtcgaa gtcctcgagg tcctcgaacg gatcggagtc
     1201 gtcggggacc agctcctcgg cgttcacgaa gatgagaccc gcgtcctcgt cgacgccgac
     1261 cgacagcgat ccgatcaccc actccggttc gaccggttcg atcagcccgg tcacgtcacg
     1321 ctcggcctcg gtggacccca gcgcctgggc gagctgaccc aggtactggc cgagcatgcc
     1381 gacctgctcc ttctccagcc ggaacgagac cacctcgttc ccccgtcggg cctgcaggta
     1441 gaagatgcgg gcgccgggtt cgccgacggt gccggggatc agtgcgtcga ggtggtcgaa
     1501 ctcgaaagat cggctggtca tgacggtgcc agtccgctca ggtcgtcgcc ggtcgagttg
     1561 accgtcagca cgatcggggt ccccggcgag ccggccgccg gcatcagcag cgcggtgatc
     1621 gagcacggcg agatgacggt gcgctggaac aggtcgaggt gcaccccggc cgccgtcgcc
     1681 accgccgcct tgatcgggtc ggcgtgcgac acggccacga cggtgtcgcc ggggtggagg
     1741 gcgaccagac gttcgatctc gttccagatg cgcagctgca tctcggcgaa tgactcgccg
     1801 ccggggaacc ggaaccgcga cggagcggtc tgcaccgtgg accactcggg cagcttgcgc
     1861 aggcgggcca acgactggcc ggtccaggtg ccgaagtcgc actcgagcag gcccttggcg
     1921 gtccggaccc gccgcccggt cgcccggccg atcggcgccg cggtctgacg ggtgcgctcc
     1981 aggggcgagg cgtagaccgc ggccacgtcg agcgccccca gacgggcggc ggcacgctcg
     2041 gcctgggcca caccggcgtc ggccaggtgc aggccggggg cccgtccggg cagcaccttg
     2101 ccggtggtcg acgtctggcc gtggcggacc agcaggaccg tcgtcggccg aggggtcttc
     2161 cgggttctcg gggcggcgcg gcgggcgggg cccgagcggg tgcgtgcggt gctcgatccg
     2221 tcggccatcg gggtggatcc tatgctcggc ccgtggagcc cctgcaggcg ctggccgccg
     2281 agatcgtcga gtgccggcgc tgcccgcgcc tggtcgagtg gcgggagcag gtggcacgag
     2341 agaagcgggc cgcgttccgc gaccaggagt actggggtcg gccggtgccc ggcttcggcg
     2401 acccggatgc tcggctgctc atcgtcgggc tggcccccgc agcgcacggc gccaaccgca
     2461 ccggccgcat gttcaccggc gaccgctccg gggacttcct gttccggtcg ttgtggcgag
     2521 ccggctacgc gaaccagccg accgcggtga gccgtgacga cggcctggag ctgaccgacg
     2581 tgttcatcac cgcaccggtc aagtgcgcgc ccccgcagaa caagccgacg acggtggagc
     2641 gcgacaactg ccgaccgtgg atcgatgccg aggtcgacct gctccgcggg ctccgggtcg
     2701 tcgtcgccct cggcgggttc ggtttcgccg aggctctgcg catcttcggc gacgggtcca
     2761 tgcgtccccg gccccgcttc ggccacggcg tcgaggtcgg cctgcccggc gggcggaccc
     2821 tcgtcggctg ctaccacgtc agccagcaga acaccttcac cggtcgtctc accgaggaga
     2881 tgctcgacgc ggtgttcgcc cggacccgtg agctgtgcgc acaggagccc accgcctgag
     2941 ccgaggctgt accgggcagg atcacccgtc ggtgcgcggg tctcgctgag ccgttcggcc
     3001 cagccgggcg ggcccaacgg accgatctcg cccggattgg tccgaacgac ccttcggtcg
     3061 gtccgctgcg gcgccgatgg agatcacatg aacgtcgccc cgaccacagt tgcggagatc
     3121 gtgccgttcg ccgagtacgg cgaggacgtc ctggccgaca acgtgttctc cggccctgcg
     3181 gatgccacac ccgcatggct cgaggggcac ctcggtcggg tcacgcagct gccgagggga
     3241 cgcaaggggc tgtggggggc gctggccacc aacgtcgtcc cgccccgggt cggcctgtcg
     3301 gctccggtcg acgccggcgg cctggcaccg gtccgtcgcc cgatgttcga cgtcgagccg
     3361 ggtctggtca tcgatctcga cgtcgcctga ccccgcccga cctcgcccga ctctcgcttt
     3421 gtcccgcaca ggcgcggtga tcaccgcgcc tgtgcgggac aaagcgagag tgggagggcc
     3481 gtgtgggagg gggagagcgc cgttgactac ggtgggcggg ttcccgttgc catccctgga
     3541 gtccctccgt gcgcccccgg tccccccgtt cgatcctcct cggcgtcgcc ctcgcgtcgc
     3601 tgctcgccct gtccgcctgc ggccagcgga cccagcccac cggctacggc gaggactacc
     3661 agaagaactt catgctcggc tgcaacgagc agaccgcggt gcccgaggag aaccccgagg
     3721 tcaccactcc gggaccggag gcccccaaga acttctgcga gtgcgtctac gacggcctgg
     3781 tcgagaaggt cccgttcgac gacgccaaga agttcgagga gcagcaggcc aaggaggacg
     3841 cgggcgacat cgcggtcccc aagccgatcc agaaggtgat cgacagctgc aacaaggaga
     3901 gctgatcgac cggcgctcag cgccagccgt atcggcgctc agcccttcga cccgggctca
     3961 gcccttcgac ctgaccgtcg ggtcgggaag cccgacccgc accgcgggtt ccgccgaggt
     4021 gggccaacgg gccctcgact tctccgccag cttgcgcatc agcgcgatgg cgcccgggtc
     4081 cgagtcgccg ggcttcgact cgatcacgcc ctggtcgatc atgcgcgaga tgatctcgcg
     4141 gcccaggtcg gtgcggacga tcgtcagggt ccagtcggag ttctcgccga tgccgccgca
     4201 ggagatgtcg gcgtgctcgg ccgcgaagtc ggggcagtac atgcacccct cgcgggtcca
     4261 ggcgtggcac tccttgaggt tgatctcgtg gtaggcgccg tcgtccatcc agatctggaa
     4321 cacgcccttg atgttcatct tcaccatgtg ctggcgggcc aggccgtact tggcccagaa
     4381 cagctcctcg aagatggcgt cgtcgaaggt cttggagcac aggaggccca ggttgaacac
     4441 gatcggcttg gagatcttgc cgaccttgcg tgaccacatg accggcggcg ccgaggactg
     4501 gcagctcatg ccgaccagcg ccaggcggga gaagccggcc tcgatcgcct cgtcgagggc
     4561 gagggtgttg gccgagtagg tgtagcggct gcccgccgcg gccaggatct gctccggcgt
     4621 cgacgccacg ccgggcttcg gtcgccacga gctgcccgcc ccgccctcgc cggcctcgag
     4681 gtacgacacg agcgcggcgt cgatgtagtc gtgctcgagc gcccacagca ggatggccga
     4741 caccagcccg ccgtcctggc ccgcggcgtt gaccgcgtcg tcgctggccc gggtgagcag
     4801 cacgtcgtgg tagatgccgg cgacctcgtc gggtcgacgc agccgtccga acaggtggtc
     4861 gtcggcctgc ccctcccata aacggaaacg ggcacacgca cgggtgcagg cggtgcagcc
     4921 cttctggccg tggatgcagt cgtcggggcc gagctcgtct tccaggtggt acgggtggta
     4981 gccgccggat tcgtggtcgt agccgagcac gtcgtggggg cacgcgatca cgcacgccgc
     5041 gcagccggtg cacaggccgg tgtcgaccac ctcggccttg agctcggccc actggttggt
     5101 ccagcgggtc gcggcgttgt cggccagacg ctgctccagg tccgccaccg gatcggtcgt
     5161 ggtcgtggtg gagcgcgccg tgtcatcccc gtcagccatg cggcggaatc tacagcgccc
     5221 ccgtcgcccc tcttccgatt ctcgccccct ccgattctcg tgaccgttcg cgccaccagt
     5281 ggcgcgaacg gtcacgagaa cggcagggac gggtcccggc tggcgcaact tgaccggcgg
     5341 gtcaagactg gggtcatgga cctgacctat ccccccgagg ccgaggagtt ccgcacggag
     5401 atccgtgcct ggctggagga gaacctgccc gacggctggt tcgacgaggg cttcgagatg
     5461 accccggagc agaagcaggc gttcaacgac gactggaccg ccaagctgtt cgagggcggc
     5521 tggatctgcg ccacgtggcc tgccgagtac ggcggcaagg gcctcaccac catgcagggc
     5581 gtggtgctgg ccgaggagtt cgccaaggcc aaggcaccca tgcgcgccga cttcttcggc
     5641 gacacgctgg tcggtccgac catcctcatg aacggcaccg aggagcagaa gaagcacttc
     5701 ctgcccagga tcctcgacgg ttccatgcgc tggtgccagg gcttctccga gcctgactcg
     5761 ggctccgacc tcgcgtcgct gaagaccacc gcggtccagg acggcgacga gtgggtcatc
     5821 aacggccaga aggtgtggac cacccaggcc cagttcgccg actactgctt cgtgctcgcc
     5881 cgcaccgacc cggatgtcaa gaagcaggca ggcatctcgt acctgctcgt ccccatggac
     5941 cagcccggca tcgaggtgcg cggcatcgtg cagcccgacg gcaccgccga gttcaacgag
     6001 gtgttcttca ccgatgcccg ctgcccgatc gacaacgtgg tcggcggcgt caacaacggc
     6061 tggaaggtcg ccaacgacac gctcggcttc gagcggggca tgtcggccac cacgggctac
     6121 cggcgcttcg ccgaggagta ccgcctgatg gtcgagaagg cgcagtccaa cggcacgatc
     6181 gacgacccga ccatccgcca gcggctgatg gagtactaca ccaagatcca gatcatgcgg
     6241 atcaacggcc tgcggtcgct caccgcgacc gtcaccggca agcgcgacat gggcgtcgcc
     6301 gcgttgggcg tgaccaacaa gatgttctgg tccgagaccc acaaagcggc aatggagctc
     6361 gccctcgaca tctggggcgc cgaggcgatg ctgtcgaccg cggctcccga cagcgggtcc
     6421 tggccggcga ccatgcgggc cgacggccgt gaggcctatc cggtcagccc gatgatgtcg
     6481 tcgttcttct tctcccgctc cgagacgatc tggggcggca ccagccagat ccagcgcaac
     6541 atcgtcggcg agaagctcct gggcctgccc cgcgaaccca agccggccgc cgcgggctga
     6601 accccgtcga cgcccatacg gcccacctga gcgtccgctc ggggctgccg cgtcgcgttc
     6661 ctcaccggca agtagcgtga ggagccgacg tcggcggccc cctgcggccc ggccggcgtc
     6721 ggcgcacccc ctgtgaacga ccgatcccca cccccgtcgc gccgccgacc cgtcggcgat
     6781 gccacccctc cccgcggggg cgtcgacgac gtcccgcggg tcgtgcgccg ccgccatcgg
     6841 gacgcggccg ggacatcggg gacaagctcc gggacctcac gccgctggcc gcgccgcgtg
     6901 ctgctcggca tcgtgtcggt gctgggggtg ctggcgctca tcgcgggtgc cggtgcggcc
     6961 tggggtctgt ggtcgttcaa ccagttcggt cgcaaggacc tggacctggc gcacgccgag
     7021 gacgacgctc cccgcaactt cctggtgatc ggctccgatt cccgcgccgg ggtccgcaag
     7081 ggcgacaagg gatcgggggt catgctcggc aagggcacgc ccggcggcca gcggtccgac
     7141 agcatcgcca tcatgcgcat cgacccgggc gaggagcgca tcgacatgtt gtcgatcccc
     7201 cgggacctgt gggtgccgat cgcggggacc ggcaaggagc agcggatcaa caccgcgtac
     7261 gccgagagca cccagacgct catcgacacg atccagtccg atctcggcat cccgatcaac
     7321 cattccgtcg aggtggacct ggtcggcttc cagaagctga tcgacgccat cggcggggtg
     7381 ccgatgtact tcccgaacgc ggtcaaggac cgcaactcgg gtctcgtggt gcagaagaag
     7441 gggtgcatga ccctcgacgg ttacatgggc ctggccttcg cccggtcccg ccacctcgag
     7501 tggagcgacg gcgtcaagtg gcacaccgac cccaccggtg acctcgggcg catgacccgc
     7561 cagcagctgc tggtgcgcac cgcgctgggg caggtcaagc acctggggct cgacgacatc
     7621 ggatcgctca aggggatcgt cgacgccgcg gtcggcagcg tgaccctcga ctccaacatg
     7681 ggcacatccg acatcatcga tctggcccag cggttctcgg acttcgaccc ggccaagctg
     7741 cagacccaca gcctgcccgt gacaggtcac accaccgatg ggggcgcctc ggtggtcttg
     7801 ctggaccagg ccgccgcgca gccggttctc gacatcttcc ggggcacgtc ggccagcgcg
     7861 gtgacgacca ccaccgagcc gcccccgtcg ccgggtgaca tcaccgtcga cgtcctcaac
     7921 ggcaccaccc gccagggcga ggcgcggcgg gtgagcttcg tgttgaccga cggcgggttc
     7981 ggcaccggca ctgtcgactc ggcgccggat ccgaccgagg ccaccgtcgt gcactacccc
     8041 aagggcggac gggcgatggc ggagctggtc gccacctggc tcgggccggc cccgaagctg
     8101 aaggaggact cgaagctggc tccggggcgt gtgacggtga cgatcggcga cgacttcgac
     8161 tcggtgtcgg aacccgcggg ggcgcccgct ccgaccgacc cgacgccggt cgacccggcg
     8221 gtggaggcgg gcgggtccgc cccgtcgacg acgaccacca cagaaccggg gtggaccccc
     8281 ggagcggtgc ccgacggggt ccgctgcggc tgacccagaa atgggaacca aactcatttc
     8341 ggcgtagatt gggcggatgg tcgaccccgc cgcccgtcgc tcccggcgcc ccgttccgcg
     8401 tgcgggcacg gtcctggtgg cgatcctggc ggtggccgcc atcgcggtgg ggctcgggtc
     8461 gtgcagcggt gacgactccg cgagcggatc tcccgagggg gaatgcccgg tcgccccggt
     8521 gtcggtcgtg gccacgatcg gtccctgggg gtcgatcgcc gaggagctcg ccggcgactg
     8581 cgcgcacgtc accaccatcg tcgacggctc ccggggtgac ccccacgacg tcgagccgac
     8641 cccggccgac cgcgccgcca tcgaggacgc ggacgtggtg atcaccaacg ggctgggcta
     8701 cgacgcctgg gccgacgacg ccctggccgc cgcgtcgtcg gagcccgccg tggtcgtcgc
     8761 cggggaggtg accggccagg agaccggcgc caacccccac gcctggtacg acccggcgat
     8821 cgtgcgggag gcggcgaccg cgatcaccca ggcgttgcag gaggcgtcgc cgaccgaggc
     8881 cgaggagtac tacacgtcgc gcttccagac ctgggagcag tcgctcgccc cctacgacgc
     8941 cgcgatcgaa cgggttcggg ccggcgccgc cgggcggccc tacgccgcca ccgagccggt
     9001 gttcgacccg atggccgccg cgctcggcct cctcgacgtc accccgtcgg gcttctcgca
     9061 gtcggtgtcc aacggggctg agccgtctcc cggcgacgtc gccgcgttcg acgccgtgct
     9121 gagcggcggg aaggccgcgg cgttgatcga caactcccag accgactcgg cgatggctcg
     9181 acagttgacc gcacgggcgg cggggagcgg cgtcccggtg gtcgaggtga ccgagaccgc
     9241 tccggcgtcg gccacgtcgt tcgtcgcctg gcaggtcgcg cagctcgacg cgctggccgc
     9301 cgcgctcggc ctgtgagcgg ggctcccgcc cccgagggaa cgaccccgac cggaggaccg
     9361 acgccgaccg ggggaccgtc gagcgatccg gtcgtgtcgc tgcgcgaggt cgaggtccac
     9421 cggggcgacc acctggtgtg gtcccggggc agcttcgaca tcgccgcggg gtcggtcgtt
     9481 ggcgtcatcg gacccaacgg gtcgggcaag tcgaccctgc tgcgcctgct gctcggactg
     9541 ctcgaaccga ccgcgggacg cgtcgaggtg ttcggcgatc cggctcggcg gggcaaccgc
     9601 cgcatcggct acgtccccca ggacgacacc gccgcccaac ccgagtcgat ccgctgccgg
     9661 gacctggtcg ccctcggcgc cggcgggcac cgcgtcggcc tgggcccctt cggagcggcg
     9721 cgccgggcga tggctgcacg ggtcgacgcc gcgctggccg agaccgcgtc gagcgcgttc
     9781 gccggccgtc gcctctcgga ggtgtccggc ggccagcgtc ggagggtgtc gctggcccag
     9841 gcgctggtcg acgaccccga gctgctgttg ctcgacgaac cgctggccaa cctcgacctg
     9901 cgccaccagc gcgagaccgt cgagctgatc gaacgggtgg cacaccgtcc gcgggccgac
     9961 ggccgggtca cgaccaccct catcgtcacc cacgacatca acccgctgct gccggtgctg
    10021 accggggcgg tgtacctgct cgacggccac ccccaccacg ccgacatcgg cgtcgtggtc
    10081 gacgagcacc tgctcagcca cctgtacggc acgtcggtgg ccgtggtccg aaccccgcag
    10141 ggcgacttgt tcacccggag cgtgtgacgt ggcgggacgg gtgctggctt cgggtgcgtc
    10201 ggtgctggcc gacatcggct accagccgga gtggacgacg gtgatggggt cgtcgttcat
    10261 gcgccacgcc ctgatcgccg ggacgctggt cgcgctggtc gccggcccga tcggctactt
    10321 cgtcgtggtg cgacgtgact cgttcgcggc gcacgccctg gcccacatcg gcttccccgg
    10381 cgccaccgcc gcggtcctgt tgggtgcccc ggtcaccgcc ggtctcgccg tgttctgcac
    10441 cgctggcggg ctggcgatcg gcctgctggg tcggcgcacg gtcgaccgtg agacggcgac
    10501 ggggacgatc ctggcgctgt cgaccgcgct tgggatcctg ttcgcgtcga tggccagcga
    10561 gggcgccgcc gcggtgaccg acgtgctgtt cggcaacctg ctcgccgtga cgacccgcca
    10621 gctctggacg ttctcgatca tggcggcggt ggtgctggtg gtgctggccg tgatcgcccg
    10681 accgttgctg ttcgcctcga tcgcccccga cgtcgccgcg gccaagggcg tggcgaccgg
    10741 ggcgctcggc ctggtgctca tggtgctgct ggcgttggtg acgacgatgg cggtgcaggt
    10801 ggtgggaacc ctgctgctgt tcgccctggt cgtcacgccc gccgcggccg cgctggcggt
    10861 gaccgcccgt ccggccgcgg cagccggtgt gagctcggcg gtggcgctgg tgagcgtgtg
    10921 gggcgggttg gtgctctcgg cggtgttcaa cctgccgccc agcttcccga tcgtggcgat
    10981 cgcgttcgtc gtctgggcgc tggcgacagc gtggtcccgc accggggcgc gccgtccgac
    11041 cggagcctga gtcgagggag tgccggcgtc ggggtcagcg cgcggggagc gtctgggggt
    11101 aggtgatgtc gatctcgggg atcgacgtcg ggaagtccga cgagttgttg gtgatgaacc
    11161 ggtcagcccc ggcgatgacc gcggtggcga ggtgggtggc gtcgacagcg cgtagccgat
    11221 gggttgcgcc gaggacggcg gccaggtcag cggtggcctc gtcgaccggt cggaggtcca
    11281 ggcgccccag catcgcggcc agcgcgtcca cctcctcgtc ggcgccgtcg cgcaggggct
    11341 tgctcaacag ctccgggacc aacaggaccg atccgatgcc ggcgacggca ccgcccgccg
    11401 gggcggcgtc gtcggggaag aggagccgga cccgtcgacc cagcgggtgg cgcggggccg
    11461 cggcgtagat gaggacatcc gcgtcgaagg cgtccaccgt cgactgctca gcgccgggac
    11521 cggctggcgg tcaccgcggc ggcgagctcg tcggctcgct cggggtcgag cgccggaacg
    11581 tccgacaggg gggtggtccg gccgcgctgc aggagtgtcc gcagccgttc ggcgtccgcg
    11641 atggccgcat ccgctcggcg gacgaggagc gcgtcgggtc ggaccagcac cgcgacctcg
    11701 tgaccgtggc gggtgatggt gatctcctcg ccggcggcca cccggtccaa cagctccggc
    11761 agcgtcgccc gggcctcgct gaccgacgtc gtcgtcatcc ccgaagtgta cagagtcgta
    11821 catccatgta cgtaccgctc ggtccggctg gccgaccggg tgctcgagtg cggttcctcg
    11881 acacggcatc accccgtgca caggttgatg tatgttgcgg tgtatgcaac gcacgaacat
    11941 ctacctcgcc gagcggcaga ccgaggcgct cgatcgcatc gccgccgacg aggggatctc
    12001 gcgcgccgag gtcatccggc gcctgctcga ccgggcgctc gccggtggcg acgacgacgt
    12061 ccgcaacgac ctcgcggcca tcgactcgtc cttcgggctc ctcgccaacc tgggcgccga
    12121 cgagctcgag ctcgatcggg acgacggaga tcggggtcgg cacctgtcgc aggtctggtc
    12181 gaccacggcg tgatcctcgt cgactccgac atcctgatcg ctcacctccg cggggtgccc
    12241 gacgcgaggt catggctgcg cgaggcgcgc ctccggtccc cgctcagcat cagcgtggtg
    12301 acggtggcgg agctgaccgg agggatgcgc tcagcggaac gccgtgaggt gtgggcgctg
    12361 ctgaacgtga tgcgcccaga gccggtcacc gagctggtcg cccgtcgggc cggtgagctc
    12421 cggcgccggt tccgacgcag ccacaccggg atcgggatcg cggactacct cgtggccgcg
    12481 accgcgctgg agctcggggc agagctggcg acgctcaacg tgacgcactt cccgatgttc
    12541 gacgacctcc gccccccgtt cgaggttccg ctgcgctgag gtgctcgacc ggtccgtgag
    12601 ccccccgtcg gcgagccccc ccggcgatcc caggcccttc atcgccgaca tccggacctg
    12661 agccgccctc aggggcctcg acgcggagct cggtgtggcg ggccagcacc tcgaagtcgc
    12721 ggtcgtggtg cagcacggcc acgtcgtggc ggatggcgac tgcggcgatg aggcagtcga
    12781 tgagcttgcg gaccgtctcg cccgagtgtc gacaccgcag gtagagcagc gcggcgagct
    12841 cgtagtcagc cgaggtggtg gccaggaccg tggtccgggc cgtgaggccg cgcagccgac
    12901 ggaggtggtc ctcgtctcgg gctccggcga gcagctccat ccggatcgcg tcggtgatcg
    12961 cgatgtcgtc gtcgagcaga gcgtcgacgc gctcgcacgc gggggagccg gtgtcgcgga
    13021 ggaactcgac ccaggcggag gtgtcgacga ggatcacggc acccgggtcg tgcgcagcgc
    13081 ctcgagctcg gcgtcccatc cggagccccg gaggtcacgg gcctgttcgg tcgacagcgc
    13141 gtcggccgcc gccagccgca gcgccaggtt caccgcgtcc cgcttggtcg ccaggtggta
    13201 ccggcgcatg acctcggcgc acgcgtcatc gtcgaggtcg atgttggtgc gtgacataca
    13261 ccaacgatac acatccggtg gctggcgaac gccggagcgg cgacgagcgt gccctcggca
    13321 ggattcgaac ctgcgcacac ggctccggag gccgatgctc tat
//
LOCUS       DAOQOD010000083        13282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1652_length_13282_cov_11.150299, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000083 DAOQOD010000000
VERSION     DAOQOD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_13282_cov_11.150299"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLZ72_09370"
     CDS             <1..757
                     /locus_tag="PLZ72_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNA36545.1"
                     /translation="VARLLAAGVVVATVGVGVAGCGDDSDAGATRGEREHKITVAGDS
                     ISVGLGSQLREVLADRAGGGGGEGGGDGLTGADDVVVKVIGEPGTGLARPDNFDWPTR
                     LRALAADFPPDVLVFSVGSNDAQDLTDAAGDVVVPMSDEQAWDAEYSRRLAEVFDAFR
                     PADGEDPATRVVWVGHVRTARDDVADTNRRVHRLATEVAADRPWVEVRDLAALLGTGD
                     TEATRCLLPDGLHLSVDCLAEADRALLGDLVAR"
     gene            807..1448
                     /locus_tag="PLZ72_09375"
     CDS             807..1448
                     /locus_tag="PLZ72_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36546.1"
                     /translation="MDRIEFFFDPVCPFAWLTSRWAVEVSERTDLRIDWRFISLRVLN
                     EERETFERHRQAHLAGLHLLRVADAVRSEHGNDGVGAYYTALGTELHTQGRRDELFGG
                     EEIDLDGLVGRALATAGLSGELAAASGDADRDRVVRAETDLALSRTGADVGTPIITFD
                     PDRPDTSSFFGPVVNRIPRGDEAVELWDALATVARIPGVAELKRSLRGTLDFT"
     gene            1622..2068
                     /locus_tag="PLZ72_09380"
     CDS             1622..2068
                     /locus_tag="PLZ72_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36547.1"
                     /translation="MEVLVGIAVMVPLTLASVSGLLLGMKVSSSTQTDQRMETELTAA
                     TEDLAATPYLICGTAGEYQQMLEAWSGKLGVERTPQEQARRPDVGVTGIEYWDAERSR
                     FRPGCPGDDGAQRLTVTVFRRDADGIDSIRGTVVKRNPDARRRGAR"
     gene            2065..3918
                     /locus_tag="PLZ72_09385"
     CDS             2065..3918
                     /locus_tag="PLZ72_09385"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA36548.1"
                     /translation="MNRREPSGAVPTGAAPSASSASRRAHVLRRRLDRRGQSGLSLLE
                     TLIGVTLSGLLLSPMMAWAAVSMQQQRSIVQRNLSGASLGVLRTVFTRDVVNADRVWV
                     DGEHLADCAVPDEGAETLMVAVTGERHTTYAVAPDPDDGGSRLVRAQCPRAGATALLA
                     NELVGDVLPGGTDVTCVTDVQVDELSDALAADAEARGSKRSDTAGGDREAGTAADPKN
                     PRCERVTLRLTTGELEQVALSAALRPRGSLVAPDPPIAAITARPVEGSRPLSVTFDGS
                     GSRDPRGVALSYSWDFGDGTTATGVRVRHDYTRTGPITATLTVTTPSGRRSSTTVVVT
                     VADNDPVATIAEPADRTKVLRGQKVAFSSKGSGDPIDAPYGGRVVSYRWDFGDGTGSD
                     EPDPVKVFSRSSPPDGFVVSLTVTDDAGRTATSRIGLVVANRAPTVALASDVDAGPAP
                     LTVTFRADVVDEPELDPAPPLKFSWDFGNGSTSDRPDPGPVLYPDAGTYTARLTVTDD
                     EGLSTTATREVVVVEPGPAAPTDLRFLRSGSQRDGRFVQMGWDRSSGARRYELELNCA
                     SCAENASGQTTSTNLRIRGLPEPSQAYDARVRAMDRDGRWGPWSPPVRVTS"
     gene            3915..>4747
                     /locus_tag="PLZ72_09390"
     CDS             3915..>4747
                     /locus_tag="PLZ72_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36549.1"
                     /translation="MSGTGAVEGGVARIRDAASARWARRGGQSGFVVMAGLAVWMLVG
                     GIVMTSLLGMTLSVAQQSKLQAEASRDARAIDGALEVAVATLQTDSTGQAGVPTGKGD
                     GSCVAGVGPRGGALVVPGDDESGGPVTVSATCSGAAGSTEVHRVVLTATTADGSGTGS
                     AALSVVAAEGGGNDVAVDSWVVDDPTVVTTTTSTVPTSSTSTSTTSTSTTSTTTTTTT
                     STTVAEDVTWALTYQSDWWFGYCADVTVSNPTGRPVDWKVAVKIDGRAYTVWNADWYQ
                     DG"
     assembly_gap    4748..4847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4910..>7168
                     /locus_tag="PLZ72_09395"
     CDS             4910..>7168
                     /locus_tag="PLZ72_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNA36550.1"
                     /translation="MRTTTPLTDGWEISTTDDGDGGAAGPWQPVTLPHCTNPTDPVDV
                     GDDHRGPAWYRRTIDVGRRDGRRVVLHVGAAGVVADVSVDGTALAQHRGAYSAFRVDL
                     TDALDAEGRGTVTIRTDNSPTDDVYPLMGDHTMFGGLYREVELIELDPVHIDVAHHAG
                     PGVVVEQTHLDDDSARLTVTVRVANDSAEPFDGSVTVTVLDAEGAPVSTAEVAATVDA
                     GQVRDVDLSIDIDRPRRWDGRRDPYLYRLVAEVADDRVEQPVGLRTFDIDADAGVRLN
                     GRPYRLYGVSRHHDVNGTPAVSRADIERDIDLIDEIGATAVRLAHYQHAEHVLDLCDE
                     RGLVVWAEIPLNAKVSGSDPVTNAASQLTELIVQQRHHPSIVCWGVQNETLISESSAD
                     PRPVIAELHALARELDPSRRTAQAQVFMAGPEDPINSLCDLNARNLYAGWYYGRAEQA
                     GDDLDRHHAANPGVPLGLSEYGADARTEYHATDPEPGDYTEDYQAAMHEVYWRLIDER
                     PWIWSSFVWNMFDFASAIRNEGGTKGFNMKGLVTRDRSVRKDAFWWYKANWSDEPVLH
                     ICSRRFVNRVDPEIDVKVYANVGPVELLVDGDPVGEPVVDGVIHRWRIRLGDGDTRVV
                     ARSGELTDEATFRRVEDADPAYVCPDPRRSAGGGGTVTSWFEESGLEVDHSRFGTWST
                     VGDLLDDPAANAVIEDTFGSAFRDHPMLDTARGISLAIVLDIAGVKMTDEDVQALHDR
                     LYALERPTGSGDG"
     assembly_gap    7169..7268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7269..8731
                     /gene="uxaC"
                     /locus_tag="PLZ72_09400"
     CDS             <7269..8731
                     /gene="uxaC"
                     /locus_tag="PLZ72_09400"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNA36551.1"
                     /translation="RRRRGTAVTGRPLRLHPDRLLPADPSTRAIARRLLEQVEPLPII
                     SPHGHCDPRWWADDEPFADPATLLITGDHYVLRMLHSAGVRLDALGRVPLDGGPVADP
                     RRVWRTFAAHYDLFHATPSRLWLDHALSVVLGADVVPDADNADRLFDELSERLAEPAF
                     RPRALFDRFGIEFLATTESPLDDLADHRAIRASGWGGNVVTAFRPDSVVDPDHPEFPA
                     NLERLAELSGIATDSWAGYLDALAERRRFFIAHGATSTDHGHPSAATADLSADDAAAL
                     FARVRAGEHTPDDAEAFRAQMLTEMVRMSLDDGLVVQLHPGSCRNHDRPGAARFGPDI
                     GADIPTRTDYVAALRPLLERFGHEPGLTLVVFTLDETTYSRELAPLAGYYPILALGAP
                     WWFHDSPEGMRRFRTDTTGTAGFANTAGFTDDTRALLSIPARHDVARRVDCAHLAGLV
                     AEHRLDEDVAARIAVELTYDRPRSVYRVDRSRFSAR"
     gene            8728..10203
                     /locus_tag="PLZ72_09405"
     CDS             8728..10203
                     /locus_tag="PLZ72_09405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HNA36552.1"
                     /translation="MSAATALDAAALAGLPPGAAPTYDRRALTPGVLHVGLGAFGRAH
                     VAHYCEDLLATGDTDAAVVGASLRTGAITAALGPQDGLYTLIESAGGEPSLRVIGAVA
                     ALAHGSEVADHVAAPATTVVTVTVTEKGYCRDPRTGGLDLDHPDVVSDLAPDRDPSRA
                     PRSLPGALVDGLQRRRRAGGADLAVISCDNLPANGEVTRAVVTDLARRRDEDLATWID
                     EHVTFRSTVVDRIVPAATDEDRELVRSRLGVVDRAPVRAEAHRLWVIEAPAPDGGVGG
                     MPAWDRVGAVFTDDLAPFQDRKLRLVNALHSAAAYLGLAAGFDTIAQAVADADLRAWL
                     DRLGVEEIEPTLPAVGHSGSGDPAGPAPAPGAATAEVLDRFANASLRHRCAQVGADGS
                     QKLGPRILATAAIRLDRGDPVVGLATVVAAWSAHVGAALGRGAALEDPLSGPLSVALE
                     RPAEADRVRALLEVITAPDALLDDDRFVAAVVAARRERGWA"
     gene            10219..11199
                     /locus_tag="PLZ72_09410"
     CDS             10219..11199
                     /locus_tag="PLZ72_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNA36553.1"
                     /translation="MTLADVARRAGVSTSTVSRALNGTAPLSPATVARVRRAVDALGY
                     SPHRSARHLAGGRTGAIAVVVPDLTNPFFATIARGAQHRAHENGELTVIADAEDSPSA
                     ELELIARLVPDVDGVVLCSPTAPTARLVAALGSRPAVLVNRPIRGRASVVADQRAAVR
                     LAHDHLRGLGHERIAYVRGPAGKWTSSTRDAQAGRLGLDLVGPVAPTFGGGARAASAL
                     PRGTTAVIAHNDLVAVGLVDALTSSGRRVPEDVSVVGCDDVELASLVRPALTTVRMPL
                     EQLGRRAVDRLAEQISGSGPVGSAPTAADPVLAVTLTVRGSTAPYAAGRT"
     gene            11196..12476
                     /locus_tag="PLZ72_09415"
     CDS             11196..12476
                     /locus_tag="PLZ72_09415"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNA36554.1"
                     /translation="MTSTDGVRVAAVGPPDGTLPPDQVRAAITDGLAGRHVGERVLVL
                     VPDHTRTAPLPFLVEVLNDVLADAATVHVMVALGTHPPLGDRELSDLVGLDRLPVADR
                     HPGLTVSNHRWRDDDELVAIGVVAEAELAAIAGDVWHPSLGGDLPLRLNRAVLDCDHV
                     ILLGPTFPHEVVGFSGGAKYFFPGVSGPEMIDVSHWLGALSGVVGTIGRVDTPTRRLV
                     DHAAAQIPTPVTLVSMVVVDDARLAGVFVGDLDAAWRRAAGLSATRHIRTLERPMQRV
                     LSMAPPMYDELWTAAKAMYKLEPVLADDAELVIFAPHLREVSRVHGAELARVGYHVLP
                     YFLEQWDRFSDVPRAVLAHSTHVRGAGTFSDGVERPRVRVTLSSRVPEAECRALGLGY
                     LDPDSVDVADWTGREDGVLVVPRAGEVLYRLAER"
     gene            12473..12997
                     /locus_tag="PLZ72_09420"
     CDS             12473..12997
                     /locus_tag="PLZ72_09420"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HNA36555.1"
                     /translation="MTGTRAEDRGPVAVVVMGVAGSGKSTVGAALADRLGWDFVDADD
                     HHPAANVDKMRRGEPLDDRDRSPWLDALRTVIDDHARRGAALVLACSALRRSYRDRLG
                     VDGDRVRLVHLDVGRPELERRLRERAGHFMAVDMLGSQLATLEVPDADEALVLDGVRP
                     VPELVRAIVSTLGC"
     gene            complement(13102..>13282)
                     /locus_tag="PLZ72_09425"
     CDS             complement(13102..>13282)
                     /locus_tag="PLZ72_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNA36556.1"
                     /translation="NMVLLCRHHHGVIHRDGWAMTVNPGPGVGEGHATITTPTGAVFH
                     TSHQRPPTQRQPAPA"
BASE COUNT         1732 a         4968 c         4596 g         1786 t
ORIGIN      
        1 tgtggcccgg ctgctcgctg cgggggtcgt cgttgccacg gtgggcgtcg gggtcgccgg
       61 ctgcggcgac gactcggacg cgggcgcgac ccgaggcgaa cgcgagcaca agatcaccgt
      121 cgccggcgac tcgatctcgg tcgggctggg atcgcagctg cgcgaggtgc tggccgaccg
      181 ggccggcggc ggcgggggag agggcggcgg cgatggtctc actggtgccg acgacgtggt
      241 cgtcaaggtg atcggcgaac ccggcaccgg tctggcccga cccgacaact tcgactggcc
      301 gacgcgcctg cgtgccctgg ccgccgactt cccgcccgac gtgctcgtgt tctccgtcgg
      361 ctccaacgac gcccaggacc tgaccgacgc ggcgggcgac gtggtcgtgc ccatgtccga
      421 cgagcaggcg tgggacgccg agtactcccg gcgtctggcc gaggtgttcg acgccttccg
      481 acccgccgac ggcgaggacc cggcgacccg ggtggtctgg gtcggccacg tccgcaccgc
      541 ccgcgacgac gtcgccgaca ccaaccggcg cgtgcaccgg ctggcgaccg aggtcgcggc
      601 ggaccgtccc tgggtggagg tgcgcgacct ggccgcgctg ttggggaccg gcgacaccga
      661 ggcgacccgg tgcctgctcc ccgacggcct tcacctcagc gtcgactgcc tggccgaggc
      721 ggatcgggcg ctgctgggcg acctggtcgc gcggtgacgg tcgagtcggg gaacaacccg
      781 tcgccgctcc ccggttggat gagctgatgg accgcatcga gttcttcttc gaccccgtct
      841 gcccgttcgc ctggctgacc agccgctggg cggtcgaggt gtcggagcgc accgacctgc
      901 ggatcgactg gcgcttcatc tcgctgcgcg tcctcaacga ggagcgggag accttcgagc
      961 gccaccggca ggctcacctg gccggcctgc acctgctgcg cgtcgcggac gcggtgcgca
     1021 gcgaacacgg caacgacggc gtcggcgcct actacaccgc gctggggacc gagctgcaca
     1081 cccaggggcg tcgtgacgag ctgttcggag gtgaggagat cgacctggac ggtctggtgg
     1141 gccgcgccct cgccaccgcc ggcctgtcgg gcgagctggc cgccgcgagc ggggacgcgg
     1201 atcgggaccg ggtcgtgcgg gccgagaccg acctcgccct ctcccgcacc ggtgcggacg
     1261 tcggaacgcc gatcatcacc ttcgacccgg accggcccga cacgtccagc ttcttcgggc
     1321 cggtggtgaa ccggatccct cggggcgacg aggcggtcga gctgtgggac gcgctggcga
     1381 ccgtggcgcg catcccgggg gtggcggagc tcaagcgctc cctgcgcggc accctcgact
     1441 tcacctgacc ccacccccgg gtttctgacg acaagtcacg acactggcgg tactcaactg
     1501 tcatcggaac gcggcgaacg cctcattcga gcgcagttcg tgccgatgag ttcccgtgag
     1561 ccggactgct cctcgtcgcc ccacgcgccc gccagcccgc ggtcagtccg gcgcgtccct
     1621 catggaggtg ctggtcggca tcgccgtcat ggtccccctg acgttggcgt cggtgtcggg
     1681 cctgctgctg ggcatgaagg tgtcgagctc cacccagacc gaccagcgca tggagaccga
     1741 gctcaccgct gccaccgagg acctggccgc cacgccgtac ctgatctgcg gtaccgccgg
     1801 ggagtaccag cagatgctgg aggcatggag cggcaagctc ggtgtcgaac ggaccccaca
     1861 ggagcaggcc cgacgtcctg atgtcggcgt caccgggatc gagtactggg acgcggagcg
     1921 gtcacggttc cgacccggct gcccgggtga cgacggggcg cagcgactca ccgtcacggt
     1981 gttccgacgc gacgcggatg ggatcgactc gatcagggga acggtcgtca agcgcaaccc
     2041 cgacgcccga cgccgaggcg cccgatgaac cgccgggagc cctccggagc cgtgcccacc
     2101 ggagctgcgc cgtccgcatc gtccgcgtcg cggcgtgcac acgtgctgcg gcggcgtctc
     2161 gatcgccgcg gtcagagcgg gctgtcgctg ctcgagaccc tgatcggcgt gacgctcagc
     2221 ggcctgctcc tcagcccgat gatggcgtgg gcggcggtgt cgatgcagca gcagcgctcg
     2281 atcgtccaac gcaacctctc gggagcgtcg ctcggcgtgc tgcgcaccgt gttcacccgt
     2341 gacgtcgtca acgccgaccg ggtctgggtc gacggcgagc acctggccga ctgcgcggtc
     2401 cccgacgagg gtgccgagac cctgatggtg gcggtgaccg gcgaacggca cacgacctac
     2461 gcggtggcac ccgatcccga cgacggcggc tcccgcctgg tgcgcgccca gtgcccgcgg
     2521 gccggtgcca ccgccctgct ggccaacgag ctcgtcgggg atgtcctgcc gggcggaacc
     2581 gacgtcacct gcgtgactga cgtgcaggtc gacgagctct ccgacgccct cgcggcggac
     2641 gccgaggccc gcggatcgaa gaggtccgac accgccggcg gcgaccggga ggccgggacc
     2701 gccgctgacc cgaagaaccc tcgttgtgaa cgcgtcaccc tgcgcctgac caccggtgag
     2761 ctcgagcagg tcgctctgtc cgcggcgctc cgtcctcgcg gcagcctggt cgcacccgac
     2821 ccgccgatcg ctgcgatcac cgcgcggccg gtcgaggggt cccgcccgct ctccgtcacc
     2881 ttcgacggtt cggggtcacg tgatccgagg ggtgtcgcgc tcagctacag ctgggacttc
     2941 ggcgacggca cgaccgccac cggggttcgg gtccgccacg actacacccg gaccggcccg
     3001 atcaccgcca ccctcaccgt gacgacgccg tcgggtcgac ggagctccac caccgtcgtc
     3061 gtgaccgtgg ccgacaacga cccggtcgcc acgatcgccg agccggcgga ccggaccaag
     3121 gtgctccgcg ggcagaaggt cgccttctcc tccaagggct ccggcgaccc gatcgacgcc
     3181 ccgtacgggg gacgggtcgt gtcgtaccgc tgggacttcg gcgacgggac aggctccgac
     3241 gagcccgacc cggtgaaggt gttcagtcgt tcgagcccgc ccgacgggtt cgtcgtctcg
     3301 ctgacggtga ccgacgacgc cgggcgcacc gcgacgtcac gcatcggcct ggtcgtggcg
     3361 aaccgggcac cgaccgtcgc cctcgcgtcc gacgtcgatg cgggaccggc tccgctgacg
     3421 gtcaccttcc gcgccgacgt ggtcgacgaa cccgagctcg accccgctcc gccgctgaag
     3481 ttcagctggg acttcggcaa cgggtccacc tcggaccgac ccgatccggg cccggtgctg
     3541 taccccgacg cgggcaccta caccgcccgg ctgaccgtca ccgacgacga ggggctgagc
     3601 acgaccgcca cccgtgaggt cgtcgtggtc gagccgggac ccgccgcgcc cacagacctc
     3661 cggttcctgc ggtccggctc gcagcgtgac ggccgcttcg tccagatggg gtgggaccgg
     3721 agctcgggcg cgcgacgcta cgagctcgag ctgaactgcg cgtcgtgcgc tgagaacgcc
     3781 tcgggccaga ccacgtcgac gaacctccgg atccggggtc tcccggagcc gtcgcaggcc
     3841 tacgacgccc gggtccgggc catggaccgc gacggtcgct ggggtccgtg gtcgccgccg
     3901 gtcagggtga cctcatgagc gggaccggag ccgtcgaggg cggcgtcgcc aggatccgcg
     3961 acgccgcgag cgcccggtgg gcccgtcgcg gcggtcagag cgggttcgtc gtgatggccg
     4021 gcctggcggt gtggatgctg gtgggtggca tcgtcatgac ctcactgctc ggcatgaccc
     4081 tctcggtcgc ccagcagtcg aagctccagg ccgaggcctc ccgcgacgcc cgagcgatcg
     4141 acggagcgct cgaggtcgcc gtggccaccc tccagaccga ctcgaccggc caggccggcg
     4201 taccgaccgg caagggcgac ggctcgtgcg tcgccggagt cggacctcgg ggcggcgccc
     4261 tggtcgtccc cggcgacgat gagtccggcg ggcccgtcac cgtctccgcc acctgttccg
     4321 gagcggccgg atcgacggag gtccaccgtg tcgtgctgac cgcgaccacc gcggacggct
     4381 ccggcaccgg gtctgccgcg ctgtcggtgg tcgcggcgga gggaggcggc aacgacgtcg
     4441 cggtcgacag ctgggtcgtc gacgatccga cggtcgtcac cacgaccacg tcgaccgttc
     4501 cgaccagctc cacctcgacc agcacgacgt cgaccagcac cacgtcgacg accacgacca
     4561 cgacgacgtc caccacggtc gccgaggacg tgacctgggc gttgacctac cagtcggact
     4621 ggtggttcgg gtactgcgcg gacgtgaccg tctccaaccc caccgggcgt ccggtcgact
     4681 ggaaggtcgc ggtgaagatc gacggccggg cctacacggt gtggaacgcg gactggtacc
     4741 aggacggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt gcaagcgctt
     4861 gcacggtcgg gttaccctga cggggcagga ccgagaccag ggggccatct tgcgcacgac
     4921 gactccgttg accgacggct gggagatcag caccaccgac gacggcgacg gtggcgccgc
     4981 cggcccgtgg cagccggtca cgttgccgca ctgcaccaac ccgaccgatc cggtcgacgt
     5041 cggtgacgac caccggggac cggcctggta ccggcgcacc atcgacgtgg gccgccgtga
     5101 cggccgacgg gtcgtgctgc acgtgggtgc ggccggcgtg gtggccgacg tcagcgtcga
     5161 cggcacggct ctggcccagc accgcggcgc ctactcggcg ttccgcgtcg atctgaccga
     5221 cgccctcgac gccgagggtc gcggcaccgt gacgatccgc accgacaact cgcccaccga
     5281 cgacgtctac ccgctgatgg gcgaccacac catgttcggc ggcctctacc gcgaggtgga
     5341 gctcatcgag ctcgacccgg tgcacatcga cgtcgcccac cacgccggcc cgggggtcgt
     5401 cgtcgaacag acccacctgg acgacgactc ggcccgcctt acggtcactg tccgggtcgc
     5461 caacgacagc gctgagccgt tcgacggctc ggtcaccgtc acggtgctcg acgccgaggg
     5521 cgccccggtc tccaccgccg aggtcgccgc gaccgtcgac gccggccagg tccgcgacgt
     5581 cgatctgagc atcgacatcg accgaccccg ccgctgggac ggtcggcggg acccgtacct
     5641 ctaccggctc gtcgccgagg tggccgacga ccgcgtcgag cagcccgtcg ggctccgcac
     5701 cttcgacatc gacgcggacg ccggagtccg cctcaacggt cggccgtacc ggctctacgg
     5761 ggtcagccgc caccacgacg tcaacggcac ccccgcggtc agccgcgccg acatcgagcg
     5821 cgacatcgac ctgatcgacg agatcggagc caccgcggtc cgcctcgccc actaccagca
     5881 cgccgaacac gtcctcgacc tgtgcgacga gcgcggcctg gtggtgtggg ccgagatccc
     5941 gctcaacgcc aaggtgtcgg ggagcgatcc ggtgaccaac gcggcgtcgc agctgaccga
     6001 gctgatcgtc cagcagcgcc accacccgtc gatcgtgtgc tggggcgttc agaacgagac
     6061 cctgatcagc gagagctcgg cggacccgcg gccggtcatc gccgagctgc acgccctggc
     6121 ccgcgagctc gatccgagcc gtcggaccgc gcaggcccag gtgttcatgg ccggccccga
     6181 ggacccgatc aactcgctgt gcgacctgaa cgcccgcaac ctgtacgccg gttggtacta
     6241 cggccgggcc gagcaggcgg gtgacgacct ggaccgccac cacgccgcca accccggcgt
     6301 cccgctcggt ctgagcgagt acggcgcgga cgcccgcacc gagtaccacg ccaccgaccc
     6361 cgaacccggc gactacaccg aggactacca ggcggccatg cacgaggtgt actggcgcct
     6421 catcgacgag cgcccgtgga tctggtcgtc gttcgtgtgg aacatgttcg acttcgcctc
     6481 ggccatccgc aacgagggcg gcacgaaggg cttcaacatg aagggcctgg tcacccgtga
     6541 ccgctccgtc cgcaaggacg cgttctggtg gtacaaggcc aactggtccg acgagccggt
     6601 gctccacatc tgctcccgac ggttcgtcaa ccgggtcgat cccgagatcg acgtcaaggt
     6661 gtacgccaac gtcggccccg tcgagctcct cgtcgacgga gacccggtcg gcgaacccgt
     6721 cgtcgacggc gtcatccacc gctggcgcat ccgcctgggc gacggcgaca cccgggtcgt
     6781 cgcccgttcc ggcgagctga ccgacgaggc cacgttccga cgcgtcgagg acgccgatcc
     6841 ggcctacgtg tgtcccgatc ctcggcgcag cgccggtggc ggcggcacgg tcacctcgtg
     6901 gttcgaggag tcgggcctgg aggtcgacca cagccgcttc ggcacctgga gcacggtcgg
     6961 cgacctgctc gacgacccgg ccgccaacgc cgtcatcgag gacaccttcg gttcggcgtt
     7021 ccgcgaccac ccgatgctcg acaccgcgcg gggcatctcg ttggccatcg tgttggacat
     7081 cgccggggtc aagatgaccg acgaggacgt ccaggcgttg cacgaccggc tctacgccct
     7141 ggaacgcccc accggctccg gcgacggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnntc cggcgacggc gcgggacggc cgtgaccggt cggccgctcc ggctgcaccc
     7321 cgaccggctc ctgccggccg acccctccac ccgcgccatc gcacgccgac tgctcgagca
     7381 ggtcgagccg ctgccgatca tcagccccca cggacactgc gacccccgct ggtgggccga
     7441 cgacgagccg ttcgcggatc cggccacgct gctgatcacc ggcgaccact acgtgctgcg
     7501 catgctgcac agcgccgggg tgcgcctgga cgccctgggg cgggtgcccc tcgacggggg
     7561 accggtcgcc gatccccgcc gggtgtggcg caccttcgcc gcgcactacg acctcttcca
     7621 cgccaccccg tcgcggctgt ggctcgacca cgccctctcc gtcgtcctcg gcgccgacgt
     7681 cgtgcccgac gccgacaacg cggaccggct cttcgacgag ctctcagaac gactggccga
     7741 gccggccttc cggccccggg cgctgttcga ccgcttcggc atcgagttcc tggccaccac
     7801 cgagtcgccc ctcgacgacc tggccgacca ccgggcgatc cgcgcgtcgg gctggggcgg
     7861 caacgtcgtg accgccttcc ggcccgactc cgtcgtcgac cccgaccacc cggagttccc
     7921 cgccaacctg gagcgcctcg ccgagctcag cggcatcgcc accgactcct gggccggcta
     7981 cctcgacgcg ttggccgaac ggcgccggtt cttcatcgcc cacggcgcta cctccaccga
     8041 ccacggccac cccagcgccg cgaccgccga cctgtcggcc gacgacgccg ccgcgctgtt
     8101 cgcccgggtc cgggccgggg agcacacgcc ggacgacgcc gaggcgttcc gggcccagat
     8161 gctcaccgag atggtccgca tgagcctgga cgacggcctg gtcgtgcagc tgcaccccgg
     8221 gagctgccgc aaccacgacc gccccggtgc cgcccgcttc ggccccgaca tcggcgccga
     8281 catcccgacc cgcaccgact acgtcgccgc gctgcgaccg ctgctcgagc gcttcggtca
     8341 cgaacccggg ctgaccctgg tcgtgttcac cctggacgag accacctaca gccgagagct
     8401 cgcgccgctc gccggctact acccgatcct ggccctcggc gcgccgtggt ggttccacga
     8461 cagccccgag ggcatgcgcc ggttccgcac cgacaccacc ggcaccgccg ggttcgccaa
     8521 caccgccggg ttcaccgacg acacccgggc gctgctgtcg atccccgccc gccacgacgt
     8581 cgcccgccgc gtcgactgcg cgcacctggc cggcctggtc gccgagcacc gcctcgacga
     8641 ggacgtcgcc gcccgcatcg ccgtcgagct gacctacgac agaccccggt cggtgtaccg
     8701 ggtcgaccgg tcccggttca gcgcccgatg agcgccgcga ccgccctgga cgccgccgcc
     8761 ctcgccggcc tcccgccagg ggccgcgccc acctatgacc gtcgagcgct cacgcccggc
     8821 gtgttgcacg tcggtctcgg cgccttcggc cgggcacacg tcgcccacta ctgcgaggac
     8881 ctgctcgcca ccggtgacac cgacgcggcc gtcgtcgggg cgtcgttgcg caccggtgcg
     8941 atcaccgccg cgctcggacc tcaggacggt ctctacactc tgatcgaatc ggccgggggt
     9001 gagccgtcgt tgcgggtgat cggcgcggtc gccgcgctgg cccacggttc cgaggtggcc
     9061 gaccacgtcg ccgcgccggc gaccacggtc gtcaccgtga ccgtgaccga gaagggctac
     9121 tgccgcgacc cccgcaccgg cggcctggac ctcgaccacc ccgacgtcgt gtcggatctg
     9181 gccccggacc gcgatccgtc ccgggcgccg cgcagcctcc ccggagcgct ggtcgacggc
     9241 ctgcagcgcc gacgccgggc gggtggcgcc gacctggcgg tgatctcgtg cgacaacctg
     9301 cccgccaacg gcgaggtcac ccgcgcggtg gtcaccgacc tggcccgtcg ccgcgacgag
     9361 gacctcgcga cgtggatcga cgagcatgtc acgttccgct ccaccgtcgt cgaccggatc
     9421 gtgcccgccg ccaccgacga ggaccgcgaa ctggtccgga gccgcctcgg tgtcgtcgac
     9481 cgggccccgg tgcgcgccga ggcacaccgg ctgtgggtga tcgaggcccc cgcccccgac
     9541 ggaggcgtcg gcggaatgcc ggcctgggac cgcgtcgggg cggtgttcac cgacgacctc
     9601 gcaccgttcc aggaccgcaa gctccgcctg gtcaacgcgc tgcactcggc cgccgcctac
     9661 ctcggcctgg ccgccgggtt cgacaccatc gcccaggcgg tcgccgacgc cgacctccgg
     9721 gcctggctcg accgtctcgg cgtcgaggag atcgaaccga ccctgcccgc ggtcgggcac
     9781 tccggctctg gggatccggc cggccctgca cccgcgcccg gagccgccac cgccgaggtg
     9841 ctcgaccggt tcgcgaacgc ctcgctgcgc caccgctgcg cccaggtcgg cgccgacggc
     9901 tcccagaagc tcggcccccg catcctcgcc accgccgcga tccgcctcga tcgcggcgac
     9961 cctgtcgtcg gactggcgac ggtcgtcgcc gcgtggagcg cgcacgtcgg agcggccctc
    10021 ggtcggggtg ccgccctgga ggacccgctg tccggtccgc tgtcggtcgc cctcgaacga
    10081 cctgccgagg ccgaccgggt gagggcgctg ctcgaggtga tcactgcccc cgacgcgctg
    10141 ctcgacgacg accggttcgt ggccgcggtc gtggccgccc gtcgcgaacg ggggtgggcg
    10201 taggtgggat cgcgagcggt gaccctcgcc gacgtggccc gccgcgccgg ggtgagcacc
    10261 tcgacggtgt cgcgagctct caacggaacg gccccgctgt cgccagcgac cgtggcccgg
    10321 gttcgccgtg ccgtcgacgc cctcggctac tcgccgcacc gctcggctcg ccacctggcc
    10381 ggcgggcgca ccggggcgat cgcggtcgtg gtgccggacc tgaccaaccc gttcttcgcc
    10441 accatcgccc ggggggccca gcaccgggcg cacgagaacg gcgagctgac cgtcatcgcg
    10501 gacgccgagg acagtccgtc ggccgaactg gagctgatcg cccggctcgt tcccgacgtc
    10561 gacggcgtcg tcctgtgctc gccgacggct ccgaccgctc gcctggtcgc cgcgctgggg
    10621 tcgcgtcccg cggtcctggt caaccgtccg atccggggtc gggcgtctgt ggtggccgac
    10681 cagcgcgcag cggttcgcct ggcgcacgac cacctgcgcg gcctcggcca cgagcgcatc
    10741 gcctacgtgc gcggaccggc cgggaagtgg acctcgagca cccgtgacgc ccaggccggc
    10801 cggctgggac tcgacctggt cggccccgtc gcccccacct tcggtggcgg agcccgggcc
    10861 gcgtcggcgc tgccgcgggg cacgaccgcg gtgatcgccc acaacgacct ggtcgcggtc
    10921 ggcctcgtcg acgccctgac gtcgtcgggt cgccgggtgc ccgaggatgt gagcgtggtc
    10981 ggctgcgacg acgtggagct ggcgtcgctg gtccgacccg cgctgaccac ggtgaggatg
    11041 ccgctcgagc agctcggccg tcgggctgtc gaccgactgg ccgagcagat ctccgggtcg
    11101 ggtcccgtcg ggtcggcccc gacggccgcg gatccggtgc tggcggtcac cctgacggtc
    11161 cgcgggtcga ccgcgccgta cgctgcgggg cggacatgac cagcaccgac ggagtccgtg
    11221 tcgcggcggt cggaccgccc gacgggacgt tgccacctga tcaggtccgg gccgcgatca
    11281 ccgacgggct ggccggccgc cacgtgggcg agcgggtcct ggtgctggtg ccggaccaca
    11341 cgcgcaccgc cccgctgccg ttcctcgtcg aggtcctcaa cgacgtcctc gccgacgcgg
    11401 ccaccgtcca cgtcatggtc gccctgggaa cccatccgcc gctgggtgac cgggagctgt
    11461 ctgacctggt cggcctggat cgccttcccg tggctgaccg gcaccccggc ctcaccgtct
    11521 ccaaccaccg ctggcgggac gacgacgagc tggtcgcgat cggtgtcgtc gccgaggccg
    11581 agctggccgc catcgccggc gacgtgtggc accccagcct gggcggggac ctgccgctgc
    11641 gcctcaaccg ggcggtgctc gactgcgacc acgtgatcct gctcggcccg acgtttcccc
    11701 acgaggtcgt cggcttctcc ggcggcgcca agtacttctt ccccggcgtg tcgggccccg
    11761 agatgatcga cgtgtcgcac tggctcggcg ctctgagcgg ggtcgtcggc acgatcggac
    11821 gcgtcgacac cccgacccgc cgactcgtcg accacgcagc tgcgcagatc ccgaccccgg
    11881 tcacgctcgt gtcgatggtc gtggtcgacg acgcccgcct ggccggcgtg ttcgtcggcg
    11941 acctcgacgc cgcgtggcgt cgggccgccg ggctcagcgc cacccgacac atccggaccc
    12001 tcgaacggcc catgcagcgg gtcctgtcga tggcgccgcc catgtacgac gagctgtgga
    12061 ccgcggccaa ggccatgtac aagctggagc cggtcctcgc cgacgacgcc gagctggtca
    12121 tcttcgcccc gcacctgcgc gaggtcagtc gggtccacgg ggcggagctg gcccgggtcg
    12181 gctaccacgt gctgccgtac ttcctcgagc agtgggaccg cttctccgat gtgccgcggg
    12241 cggtgctggc gcactccacc cacgtgcgcg gcgccggcac cttctccgac ggcgtcgaac
    12301 ggccccgggt gcgggtcacc ctctccagcc gggtgcccga ggccgagtgc cgggccctgg
    12361 gcctcggcta cctcgacccc gactcggtcg acgtcgccga ctggaccgga cgcgaggacg
    12421 gcgtgctggt ggtgccccgc gccggcgagg tgctctaccg gttggcggag cggtgaccgg
    12481 gacccgcgcc gaggaccgcg ggccggtggc ggtcgtggtg atgggggtgg cgggcagcgg
    12541 caagagcacc gtcggcgccg cgctggcgga ccggctgggc tgggacttcg tcgacgctga
    12601 cgatcaccac ccggccgcca acgtcgacaa gatgcgccgc ggcgaaccgc tcgacgaccg
    12661 ggaccgctcg ccgtggttgg acgcgctgcg gaccgtcatc gacgaccacg cgcgccgagg
    12721 cgccgcgctg gtcctggcct gttcggcgtt gcgccggtcc taccgcgacc gcctcggcgt
    12781 cgacggcgac cgggtccgtc tggtccacct cgacgtcggg cgccccgagc tggagcggcg
    12841 gctgcgggaa cgcgccgggc acttcatggc cgtcgacatg ttgggcagcc agctcgccac
    12901 cctcgaggtc cccgacgcgg acgaggcgct ggtgctcgac ggcgtccgac cggtacccga
    12961 gctggtccgg gccatcgtgt cgaccctggg gtgctgacgg ctcgacgagg ctctgccggt
    13021 accctcatct ggcccgcgtg ggggcgcggt gaatcgaccg tcgacgggga gttccgttgg
    13081 tccgctcggg tcccgcgctg ctcaggcggg agcgggttgt cgctgggtcg gtggccgttg
    13141 gtgcgaggtg tggaacaccg cgccggtcgg ggtggtgatg gtggcgtgcc cctcaccgac
    13201 accagggccg gggttgacgg tcatggccca gccgtcgcga tggatcacac cgtggtggtg
    13261 gcgacagagg aggaccatgt tg
//
LOCUS       DAOQOD010000084        13033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1690_length_13033_cov_12.631916, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000084 DAOQOD010000000
VERSION     DAOQOD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_13033_cov_12.631916"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..2620)
                     /locus_tag="PLZ72_09430"
     CDS             complement(119..2620)
                     /locus_tag="PLZ72_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA36557.1"
                     /translation="MTDTTPAPLHPIPGDLSSDDPRLMMLLDASVDALMVMDDSARVV
                     WISPSTEQMLGRTLADLRGSNALDLVHPDDLQEAAVLLGRMVDDGVDDDPPAIVRLIT
                     GDGSEVWAEIKGSDLRDVPGIDGVVLSARDISYRFALEEALQQSQRRFEALIHNSNDA
                     IVLLDADLQLTYVGPSIERLTGFPPRAMVGLDVSDVVLEPGRTDLLEALDLVTTDPDR
                     VESLRVRTRHRDGSDRWFGVRVSNKLGDPAISGIIANLRDVTDVVAAEAEAARLTEIF
                     DLTDDLVVVVDGDQTLRYMNPACARFSGIDPAHIPLGRTWDGPRLADTQPGSCPLGNG
                     ERRWTVESTLTRHDGVEVPFSVQVIAHQDPDGRTSRYSAVAHDISESKDLAASLERQA
                     RHDPLTGLPNRSMLEDRMRQLRDCCGPGCPVALLFIDLDNFKVINDSLGHDFGDRLLQ
                     AVAQRLRSVVRPGDTVARFGGDEFVILCDGLTGVEAAENVARRITESMLDPIVVDRSP
                     VHVSVSIGIAEERGDLPDADPIALIRDADTAMYRAKADGRGRVAVFDDELRTRAVERQ
                     QIESALRQAPFDGSLVLHYQPQVDLLSGRLLGVEALVRWRHDGQLLGPGEFIGIAEET
                     DLIVPLGAAVLETACRDLAAWQRMAGWEHLGVSVNVSVRQLQQPGFVDLVDDVLRRTG
                     LTPGTLTLEITESVLLDDAALAELHMDHLKSMGVVVAIDDFGTGYSSLTYLARLPVGI
                     VKLDRAFLEPIGSDLDRSDLVAGILDLVTTMGMDCMAEGIESEDQYLLLAGLGCGMGQ
                     GYHIARPMPAEDFVARLGLLDPHDSWPRGAVPATT"
     gene            2713..3639
                     /locus_tag="PLZ72_09435"
     CDS             2713..3639
                     /locus_tag="PLZ72_09435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA36558.1"
                     /translation="MSDDPPGPDPAPDSGDPDAPRVVRPRHTPVPRRASRRRWAILAG
                     VVALVVAATVTVILAGRVRPGGHDARGGDPPPSDSSTTSIGSSTSAPTTTTTNPPNDL
                     LAPPPTVVPPSVDADGMAPLVRRVDTTDRVVFITIDDGNIRNPSYPDHLARLGVPFTS
                     FLTQPMAKADPAYWKSMVERGGTIQTHTISHPNLRTVGAERMRREICEPVMTFTALFG
                     ARPTLFRPPYGNSSDAVRRVAAECGYAAVVLWTGSTNVGKLTMQDVVLKPGDIILMHY
                     RDTLDADLDDLVARVRAEGFRIGRLEDYLRAQ"
     gene            complement(3673..5340)
                     /locus_tag="PLZ72_09440"
     CDS             complement(3673..5340)
                     /locus_tag="PLZ72_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36559.1"
                     /translation="MTHPHSRTSRRRALGVAAAVLVASMLIAACGGSSESGNGGGSGD
                     AAAGTPTPGGTVTYGLEAENAGGWCLPEAQLAISGIQVARAIYDTLTAPDEHAVYQPY
                     LAESVTPNDDFTQWTIVLRDGVTFHDGSPLDATVVKNNVDAWRGVYPGRSPLLFTFVY
                     EPISAVDVVDAATVKVTTKTSWPSFPAYLFNDGRSGIIAQAQLDDPVSCDRKLIGTGP
                     FQLVEWKPNDHLTAERNANYWQKDASGNQLPYLDQITFRPITDPTSRLNALLAGELNA
                     MHEAGAEVLDQMRQDAEQGKIAVTATTDYTEVAYEMFNVSKPPFDNRDARLAAIYAID
                     REQLNEVRNLGLTKMASGPFAPGEIGYLEDTGYPSYSKKKAKEHLDAYTAATGKPLEF
                     TLVGTPDPGTTKLLQFLQEQWKDSGITANIRTVEQAVLINTALSDDWNVIDWRNHPGG
                     NPDAQYIWWKGGSTVNFNKFDDPEMNALLDAGRAEPDKEKAAGIYQDINRRFATEGYN
                     LWLQWVEWSIGTAPDVHGVYGPDLPDGGKPFTGLATGHPVLGLWVQQ"
     gene            5525..5797
                     /locus_tag="PLZ72_09445"
     CDS             5525..5797
                     /locus_tag="PLZ72_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36560.1"
                     /translation="MRDDRSGPPEDDVAPVALLLSVIGLLGCFPVSLTALAIAYLSRE
                     RIRKSDGALGGERTVARAIVLGWVGVGVSVVVFLVFAVAAVIRRGT"
     gene            5934..6689
                     /locus_tag="PLZ72_09450"
     CDS             5934..6689
                     /locus_tag="PLZ72_09450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36561.1"
                     /translation="MSTERFVGKVALITGAGSGIGRAVAGRLVDEGATVFAVDISADG
                     LAALADEVSGPGAVLTHRADLSEPSACAGAVAECVDRAGRLDVLGNIAGIAASSHLAD
                     VTVEQYRRIMAINTDACFFMCQAAVPHLLETGGNIVNLASNAGLMGQAYTVAYCMSKG
                     AVIQLTRSLAMEFVRTDLRVNAIAPAAVNTPLLHNFEIADGVDVELMKPYMGFRGMAE
                     ADELARLFAFVASDEVPNMHGAVISVDNGITAG"
     gene            complement(6676..7806)
                     /gene="ald"
                     /locus_tag="PLZ72_09455"
     CDS             complement(6676..7806)
                     /gene="ald"
                     /locus_tag="PLZ72_09455"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNA36562.1"
                     /translation="MDALTVGIPTEVKAEEHRVAITPDGVRELLHAGAAVLVQSGAGA
                     DSSIPDDDYRRAGATIVTDAAEVWERADLVCKVKEPLESEFVHFRDDLVLFTYLHLAA
                     YPAVADALLDAGVTGIAYETVQLPDGSLPLLAPMSEVAGRMSVQIGAHFLERHNGGRG
                     VLLGGAPGVRPARVVVLGAGNVGWNAAWMAAGLEAEVDLLDKSIDRLRHVDQIQMGRI
                     TTLSSNRGAVERCVAEADLVIGAVLVPGGRAPVVVSEDMVRSMRPGAVIIDIAIDQGG
                     CVETSHETTHAAPTFLVHDVVHYAVGNMPGAVPHTSTHALTNATLPYLTELARRGARD
                     AARRDPALACGVNTLGGHVVNPTVADALGRSATPLDDLLSPR"
     gene            7967..8776
                     /locus_tag="PLZ72_09460"
     CDS             7967..8776
                     /locus_tag="PLZ72_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36563.1"
                     /translation="MTDLTLNSLFSVEGKVAVVTGGSRGIGEMVAAGLLANGATVLIS
                     SRKAEVCNATAARLAETYGGTCIPLPADVSDLAGVDALVAAVREHTDHVDILVNNAGV
                     SWGAPLEEFPEKGWDKVFDTNVKGVFFLTQRLLPLLEARASAEDPSRVVNIGSIDGIR
                     TPVFDTHSYGPSKAAVHALTRQMAAKLVKRHVLVNAIAPGPFPTWMLSTGVGTGGDVD
                     GTDWEAVGAMTPRGRVGSPEDIAGLTIFLCSRAGAYTVGEVITCDGGAVVS"
     gene            8825..9691
                     /locus_tag="PLZ72_09465"
     CDS             8825..9691
                     /locus_tag="PLZ72_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA36564.1"
                     /translation="MRYHLFLPQMRMTHDQIVERALAAEAAGFEGVAFMDHLAPPLAV
                     EAPMWEAMQVAGWVLARTSTLRVGHLVLCDSLRHPAVLARQVTSLDHASGGRFELGLG
                     WGSVPEELVAFGVGSAEPSARVGRLAETLAILEALWSGEAVTFRGEHFTLTDARQQPP
                     PTGPIPLTIGGSGRRTMELVRRHATWWNLPVHRVDRLEQLRPAAGDARVSVQVMVALL
                     GDGADRGAVAEMVARRYGTGGLESRVVLGTADEVAGHMDELAGRGVERVHVWFADFAP
                     PSTIDAFSAVIG"
     gene            complement(9696..11324)
                     /locus_tag="PLZ72_09470"
     CDS             complement(9696..11324)
                     /locus_tag="PLZ72_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA36565.1"
                     /translation="MAGAAADSAVMSLGSSDTDLADLDGPEDLVRRLLEVARTRTEMD
                     MVWVSRINGDVLSLAIVNGALGDSTILEGSTIPLKDSFCAQVIGGRIASWVPDVRALP
                     ETRDLPMAAQLGIRAFVSVPIKAATGSVIGMLTAVSTDPRDVDERTRDFLQMLADSAS
                     DLLRDVPIDGDDRRRLRDRIGRVIDHDLLTIAFQPIVDLEHRRVAGYEALSRFPGEPA
                     RPDQWFADAAAVGLGVALELHAARTAVSALANLPPDTYLSINLSPAALVSVDPSELVP
                     ADPTRLVLELTEHARVGDYEQLRHALDRFRGAGVRIAVDDAGAGFASFRHILELEPDI
                     VKVDLAISQGIHRDPARQALVGGISELARHSGATLVAEGVEEPADLDRLATLGVSIFQ
                     GYLFGRPAPLPSNGLPEAAIPAHTIGTPAHRSRRVMESRFETAVLSAAVPTAIVDLDG
                     TMRSVSDRFAAFLGRTTAEMTGRRFQELTHPEDLDADVEHLRACLEGRADGYRMTKRY
                     LRPDGSPVSADLIATVVRDRDGAPLYFVSHVLPL"
     gene            complement(11371..12327)
                     /locus_tag="PLZ72_09475"
     CDS             complement(11371..12327)
                     /locus_tag="PLZ72_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36566.1"
                     /translation="MTAGPPPTPHGGTGEADGTDGTDDAVAATRTDLIGSDGLRLAAD
                     VAGPDDGPTVVLLHGGGQTRHSWAGTWRYLADHGWRAWTVDLRGHGDSEWSGDGDYQL
                     QAFAADTTAVAGAVTAATGRPPVLVGASLGGLSALVAVAEADPQDGLARALVLVDVAH
                     RLESAGTDRIGAFMTGNLDGFESLDEAADVIAAYNPHRPRPTNLDGLAKNLRQGPDGR
                     WRWHWDPRFVSGKFGAADETRATIVGPDLLERSADALRVPTLLVRGRSSDLLSEEGAR
                     DFLARVPHAEFADVAGAGHMVVGDRNEVFNSAVLEFLDRHRG"
     gene            complement(12324..>13033)
                     /locus_tag="PLZ72_09480"
     CDS             complement(12324..>13033)
                     /locus_tag="PLZ72_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA36567.1"
                     /translation="HGAWQGAWCWSRLQVSLRGLGALSIAVDLPGHGEDRTPIAGLTL
                     SDYADRVIQVLDGIDEPVTLVGHSLGGATISTVAERRPERIAGLVYLCALLQPSGATP
                     ADLHRHDPDSALVAAIEMAPDGSATSIRPDAAAELFYGDCRPEDVRTAIEHLVPEPIG
                     PAMSTIDTSAERWGSVPRAYVRCDRDRAIVPAEQDRMIAEVGVDVVVEMASSHSPFLS
                     QPDALAAALVEIRDGTA"
BASE COUNT         1808 a         4407 c         4846 g         1972 t
ORIGIN      
        1 gtcgctgtgg tggcgtgtgg gctacaagtt cggtgggcgg cggcgatgtt gtagccgggg
       61 gtcgctgtgg tggcgtgtgg gctacaagtt cggtgggcgg cgccgacagc cgcgcgggtc
      121 aggtggtggc gggaaccgcg ccgcggggcc agctgtcgtg cgggtcgagc agcccgagtc
      181 gggcgacgaa gtcctcggcc ggcatcggtc gggcgatgtg gtacccctgg cccatcccgc
      241 accccagtcc cgccaggagc aggtactggt cctcggactc gatgccctcg gccatgcagt
      301 ccatccccat ggtcgtcacc aggtcgagga tcccggcgac caggtcgctg cggtcgaggt
      361 cggagccgat cggctcgaga aacgcccggt ccagcttcac gatccccacc gggagtcggg
      421 ccaggtaggt caacgacgag tagcccgtcc cgaagtcgtc gatggcgacg accacgccca
      481 tggacttcag gtggtccatg tgcagctcag ccagagcggc gtcgtcgagc aggaccgact
      541 cggtgatctc cagggtgagg gtgcccgggg tgagtcccgt ccgtcggagc acgtcgtcga
      601 ccaggtcgac gaagcccggc tgctggagct gacgcaccga cacgttgacc gacacgccga
      661 ggtgctccca gcccgccatc cgctgccagg ccgccaggtc ccggcacgcc gtctcgagga
      721 ccgcggcgcc cagcgggacg atgaggtcgg tctcctcggc gatgccgatg aactcaccgg
      781 ggcccaggag ctgaccgtcg tggcgccagc ggaccagcgc ctcgacgccg agcaggcggc
      841 ccgagagcag gtcgacctgc ggctggtagt gcaggacgag ggacccgtcg aaaggggcct
      901 ggcgcagggc ggactcgatc tgctggcgct cgacggcacg ggtgcgaagc tcgtcgtcga
      961 acaccgcgac gcggccccgg ccatcggcct tggcccggta catcgcggtg tcggcgtcgc
     1021 ggatcagggc gatcggatcg gcgtcgggca gatcgcctcg ctcctcggcg atgccgatcg
     1081 acacgctgac gtgcaccggg ctcctgtcga cgacgatcgg gtcgagcatc gactcggtga
     1141 tccggcgggc gacgttctcg gcggcctcga cgccggtgag gccgtcgcag aggatgacga
     1201 actcgtcgcc gccgaagcgg gcgacggtgt cacccggacg cacgacgctg cgcaggcgct
     1261 gcgcgacggc ctggagcagc cggtcgccga agtcgtggcc gaggctgtcg ttgatgacct
     1321 tgaagttgtc gaggtcgatg aacagcagcg ccaccggaca gccgggaccg cagcagtcgc
     1381 ggagctgacg catcctgtcc tcgagcatgc tgcggttggg aaggccggtg agcgggtcgt
     1441 ggcgggcctg tcgttcgagc gaggccgcca gatccttgct ctcgctgatg tcgtgagcaa
     1501 cagccgagta tcgcgacgtt cgaccgtcgg ggtcctggtg ggcgatgacc tgcacagaga
     1561 acgggacctc gacgccgtcg tgacgcgtca gagttgattc gacggtccag cgccgctctc
     1621 cgttgccgag cgggcacgag cccggctggg tgtccgcgag ccgaggccca tcccacgtcc
     1681 ggccgagcgg gatgtgggcc gggtcgatcc cggagaagcg ggcacacgcc gggttcatgt
     1741 agcggagggt ctggtcgccg tcgacgacca cgaccaggtc gtcggtgagg tcgaagatct
     1801 cggtgaggcg ggcggcttcg gcctcggcag cgaccacgtc ggtgacgtcg cggaggttgg
     1861 cgatgatgcc cgagatcgcc ggatcgccca gcttgttcga cacccgtacg ccgaaccaac
     1921 ggtcggagcc gtcgcggtgt cgggtccgga cccgcagcga ctccacgcgg tcgggatccg
     1981 tcgtcaccag gtcgagcgcc tccagcaggt cggttcggcc cggctcgagg accacgtcgg
     2041 agacgtcgag gccgaccatg gcccggggag ggaagccggt caaccgctcg atcgacggcc
     2101 ccacgtaggt gagctggagg tccgcatcga gcaacacgat ggcgtcgttg ctgttgtgga
     2161 tcagcgcttc gaaccgtcgt tgtgactgct gcagggcctc ctcgagggcg aatcgatagg
     2221 agatgtctcg tgctgagagg acgacgccgt cgatgccggg cacgtcgcgc aggtccgatc
     2281 ccttgatctc ggcccacacc tcggacccgt caccggtgat cagccggacg atggcgggtg
     2341 ggtcgtcgtc gacgccgtcg tccaccatgc ggccgagcag gaccgccgcc tcctgcagat
     2401 cgtcgggatg gaccagatcg agtgcgttgg atccccgcag gtcagccagc gtccgcccca
     2461 acatctgctc ggtgctcggg ctgatccaca ccacccgggc cgagtcgtcc atcaccatca
     2521 gcgcatcgac cgacgcgtcg agcagcatca tcagacgcgg gtcgtcggag gacagatcgc
     2581 ccgggatcgg gtggagggga gcgggggtgg tgtcggtcat ggcggcgcgg atcgttccgt
     2641 gcgtcagaca tcggtcgtga cccgccgtgg gctgagaact tcgggtcctt cgacccacta
     2701 gcttcggcgg ccgtgtcgga cgatccgccc ggaccggacc ccgccccgga ctccggggat
     2761 ccggatgcgc ccagggtggt ccgtcctcgc cacaccccgg tcccgcggcg agcgtcccgc
     2821 cgtcggtggg cgatcctggc cggcgtggtc gccctggtcg tcgcggcgac ggtgacggtg
     2881 atcctcgccg gccgagtccg gcccggcggt cacgacgccc ggggcggtga cccaccgccc
     2941 tccgattcgt cgacgacgtc gatcgggtcg tcgacgtctg cgcccacgac gaccaccacg
     3001 aacccgccga acgacctgct ggcgcctcct ccgaccgtgg tccccccgtc ggtggacgcc
     3061 gacgggatgg cgccgctggt caggcgggtc gacaccaccg accgcgtcgt gttcatcacc
     3121 atcgacgacg gcaacatccg caacccgtcc tatccggacc acctggcccg gctgggcgtc
     3181 ccgttcacat cgttcctgac ccagccgatg gccaaggccg atccggccta ctggaagtcg
     3241 atggtcgagc gcggcgggac catccagacg cacacgatca gccatcccaa cctccggacg
     3301 gtcggtgcgg agcggatgcg gcgcgagatc tgcgaaccgg tgatgacctt cacggcgttg
     3361 ttcggggcgc ggcccaccct gttccgcccg ccctacggca actcatccga cgcggtccga
     3421 cgggttgccg cggagtgcgg ttacgccgcg gtggtgctgt ggacggggtc gaccaacgtc
     3481 gggaagctga ccatgcagga cgtcgtgttg aagcccggcg acatcatcct catgcactac
     3541 cgcgacacgc tcgacgccga cctggacgac ctggtcgccc gggtgcgcgc cgaggggttc
     3601 cggatcggtc gcctggagga ctacctgcgg gcgcagtgaa gcgctcgcct cactgcgcca
     3661 cggcgtcaga ggtcactgct gcacccagag ccccagcacg gggtggccgg tcgccaggcc
     3721 ggtgaacggc ttgccgccgt cgggaaggtc gggtccgtac acgccgtgga cgtcgggcgc
     3781 ggtgccgatg ctccactcga cccactggag ccacaggttg tagccctcgg ttgcgaagcg
     3841 ccggttgatg tcctggtaga tgccggcggc cttctccttg tcgggttcgg cccgccccgc
     3901 gtcgaggagc gcgttcatct cggggtcgtc gaacttgttg aagttcaccg tcgacccgcc
     3961 cttccaccag atgtactggg cgtcgggatt gccgccgggg tggttgcgcc agtcgatcac
     4021 gttccagtcg tcggacaggg cggtgttgat caggacggcc tgttcgacgg tgcggatgtt
     4081 ggcggtgatc cccgagtcct tccactgctc ctggaggaac tggaggagct tcgtcgtgcc
     4141 cgggtccggg gttccgacca gggtgaactc gagcggcttg ccggtggcgg cggtgtaggc
     4201 gtcgaggtgc tccttggcct tcttcttgct gtaggacggg tagccggtgt cctcgaggta
     4261 gccgatctcg ccgggtgcga acgggccgga cgccatcttg gtcagcccca ggttccggac
     4321 ctcgttgagc tgctcgcggt cgatggcgta gatggccgcc aggcgggcgt cccggttgtc
     4381 gaacggcggc ttggagacgt tgaacatctc gtaggcgacc tcggtgtagt cggtggtcgc
     4441 ggtgacggcg atcttgccct gctcggcgtc ctggcgcatc tggtcgagca cctcggcgcc
     4501 ggcctcgtgc atggcgttca gctcgccggc gaggagggcg ttcagccggc tcgtcgggtc
     4561 ggtgatcgga cggaaggtga tctggtccag gtagggcagc tggttgccgg acgcgtcctt
     4621 ctgccagtag ttggcgttgc gctcggcggt gaggtggtcg ttgggcttcc actcgaccag
     4681 ctggaacgga ccggttccga tcagcttgcg gtcgcagctc acggggtcgt cgagctgggc
     4741 ctgggcgatg atcccggagc ggccgtcgtt gaacaggtag gccgggaacg acggccacga
     4801 cgtcttcgtc gtgaccttca ccgtggcggc gtcgacaacg tcgacggcgc tgatcggttc
     4861 gtacacgaac gtgaacagca gggggctgcg tccggggtac acgccgcgcc aggcgtcgac
     4921 gttgttcttg accaccgtcg cgtcgagagg tgatccgtca tggaacgtca ccccgtcgcg
     4981 caggacgatg gtccactggg tgaagtcgtc gttgggggtg accgactcgg ccaggtacgg
     5041 ctggtacacg gcgtgctcgt cgggcgcggt cagggtgtcg tagatggccc tggccacctg
     5101 gatgcccgag atcgcaagct gggcctctgg taggcaccag ccgcccgcgt tctcggcctc
     5161 gagtccgtag gtcacggtgc cgccgggggt cggggtcccg gcagccgcgt cgccggagcc
     5221 gccgccgttg cccgactccg aggacccgcc gcatgcggcg atcagcatgg aggcgacgag
     5281 caccgcggcg gccacgccca acgctcggcg tcgcgacgtc cgggaatggg ggtgggtcac
     5341 cgttcctcct cgttggacgg gcccggcgat cgggccgccg tgtccggatc cggagatcct
     5401 gcccggtcgg cgccgcggag gggagggtcc ggcgacgtga catctctcac cgggtccggc
     5461 tcccaccgct gtttcgagta tccccggaat cgtggcgggg gcccgggtcg ggcaagctgg
     5521 gccgatgcgc gacgacaggt cgggaccacc cgaggacgac gtcgcgccgg tcgccctgct
     5581 gctgtcggtg atcggactcc tggggtgctt ccccgtctcg ctcaccgcac tggcgatcgc
     5641 ctacctgtcg cgggagcgga tccggaagtc cgacggggct ctgggcggcg agcgcaccgt
     5701 ggcgcgggcg atcgtgctgg ggtgggtcgg cgtcggagtg tcggtggtcg tcttcctggt
     5761 cttcgcggtc gccgcggtga tccggcgggg aacctgaccc gacgcggtga tcggtcgccg
     5821 ggcgggggag cggtacggtt ccggcccggc cccggcgtcg gccgtcgacg ccacccggca
     5881 gtgatcgctc cggaccgatc ggcggtcggg cgtccgcacc acagggagag cacatgagca
     5941 ccgaacgctt cgtcggcaag gtcgcgctga tcaccggggc cggttccggc atcggtcggg
     6001 ctgtggcagg tcgtctggtc gatgaaggcg cgacggtgtt cgcggtcgac atctcagccg
     6061 acggcctggc cgccctggcc gacgaggtgt ccggacccgg agcggtgctc acccaccgtg
     6121 ccgacctgtc ggagccgtca gcgtgcgccg gcgcggtggc cgagtgcgtg gatcgggccg
     6181 gccggctcga cgtgctgggc aacatcgcgg gcatcgcggc gtcgagccac ctggctgacg
     6241 tgaccgtcga gcagtaccgc cgcatcatgg cgatcaacac cgacgcctgc ttcttcatgt
     6301 gccaggccgc cgtgccgcat ctgctcgaga ccggcggcaa catcgtgaac ctggcctcga
     6361 acgccgggct gatgggccag gcgtacacgg tcgcctactg catgtcgaag ggggcggtga
     6421 tccagctcac gcgctcgctg gccatggagt tcgtccggac cgacctgcgg gtcaacgcca
     6481 tcgcacccgc ggcggtcaac accccgctgc tgcacaactt cgagatcgcc gacggcgtcg
     6541 acgtcgagct catgaagccc tacatgggct tccggggaat ggccgaggcc gatgagttgg
     6601 cccggctgtt cgcgttcgtc gcctccgacg aggtgccgaa catgcacggt gcggtcatca
     6661 gcgtcgacaa cgggatcacc gcgggctgag cagatcgtcg agcggggtcg ccgaacgtcc
     6721 gagggcgtcg gcgacggtgg ggttcaccac gtgcccgccg agcgtgttca ccccgcaggc
     6781 cagggccgga tcccgtcgcg ccgcgtcccg ggccccccga cgggcgagct cggtgaggta
     6841 ggggagcgtc gcgttggtca gcgcgtgggt agaggtgtgc gggaccgccc cgggcatgtt
     6901 gcccaccgcg tagtgcacga cgtcgtggac caggaacgtc ggagcggcgt gggtggtctc
     6961 gtggctggtc tcgacgcagc cgccctggtc gatggcgatg tcgatgatca cggcgccggg
     7021 gcgcatcgat cggaccatgt cctcggacac gacgaccggg gcgcggccgc ccgggaccag
     7081 gaccgctccg atcaccaggt cggcctccgc gacgcagcgt tcgacggcgc cccggttgga
     7141 gctgagggtc gtgatgcggc ccatctggat ctggtcgacg tgacgcaggc ggtcgatgct
     7201 cttgtcgagc aggtcgacct cggcctcgag cccggcggcc atccacgccg cgttccaccc
     7261 gacgttgccc gcgccgagaa ccaccacgcg agcgggccgc acgccggggg cccctcccag
     7321 gagcacccct cggcccccgt tgtggcgctc caggaagtgg gcgccgatct ggacgctcat
     7381 ccggccggcg acctcgctca tgggggcgag cagggggagc gacccgtcgg gcagctgcac
     7441 ggtctcgtac gcgatgccgg tgacgccggc gtcgagcagg gcgtcggcca cagcggggta
     7501 cgcggcaagg tggaggtagg tgaacaggac gaggtcgtcg cggaagtgga cgaactcgga
     7561 ctcgagcggc tccttgacct tgcagaccag gtccgcgcgc tcccacacct cggcggcgtc
     7621 ggtcacgatc gtggcgccgg cccggcggta gtcgtcgtcg gggatcgacg agtcggcccc
     7681 ggcgccggac tggaccagaa cggcggcccc ggcgtgcagc agctcgcgga cgccgtcggg
     7741 ggtgatcgcc acccggtgct cctcggcctt gacctcggtc ggtatgccga cggtgagtgc
     7801 atccatcgca gtgatctccg tgggtcgggg tcgccgggaa ccgacgacgg ggtcgagtct
     7861 tgcaaaccgc ggacccgacg cgtcgtccgg cgcgcggggc gtacgctccc ggtcctcgga
     7921 acggcccacc gtgggcggtt cctcgccgac tccgggagct cccacgatga ccgacctgac
     7981 gctcaactcg ctgttctccg tcgagggcaa ggtcgccgtg gtcaccggcg gctcccgcgg
     8041 catcggcgag atggtcgcgg ccggcctgct ggccaacggc gccaccgtgc tcatcagctc
     8101 acgcaaggcc gaggtgtgca acgcgactgc ggcccggctg gcggagacct acggcgggac
     8161 ctgcatccct ctgccggcgg acgtgtcgga cctggccggc gtggacgcgc tggtcgccgc
     8221 ggtgcgagag cacacagacc acgtcgacat cctggtcaac aacgcaggcg tgtcgtgggg
     8281 cgcgccgctc gaggagttcc ccgagaaggg ctgggacaag gtgttcgaca ccaacgtcaa
     8341 gggcgtgttc ttcctcacgc agcggctcct gcccctcctc gaggcccgag cgagcgccga
     8401 ggacccgtcg cgggtggtga acatcggctc gatcgacggc atccgcacgc cggtgttcga
     8461 cacccactcc tacggcccgt ccaaggccgc cgtgcacgcc ctcacccgtc agatggcggc
     8521 aaagctcgtc aagcgacacg tgctggtcaa cgccatcgcg cccgggccgt tcccgacgtg
     8581 gatgctcagc accggcgtcg gcaccggcgg cgacgtggac ggcaccgact gggaggcggt
     8641 cggcgcgatg acgccccgcg gtcgggtcgg cagtcccgag gacatcgccg ggctgaccat
     8701 cttcctgtgc tcgcgggccg gggcctacac cgtcggtgag gtcatcactt gcgacggcgg
     8761 agcagtcgtg tcctgatcca ggctcgtgtc ctgatccagg ctcctgatcg gaggaaaccg
     8821 atcgatgcgc taccacctgt tcctgccgca gatgcgcatg acccacgacc agatcgtgga
     8881 gcgcgccctg gccgccgaag cggccgggtt cgagggggtc gcgttcatgg accacctcgc
     8941 cccgcccctg gcggtcgagg cccccatgtg ggaggcgatg caggtggccg gctgggtcct
     9001 ggcccggacc tcgacgctgc gggtcgggca cctggtgctg tgcgactccc tgcgccatcc
     9061 ggcggtgctg gcccgtcagg tgacctcgct cgaccacgcg tccggaggac ggttcgagct
     9121 gggcctcggt tgggggtcgg tccccgagga gctcgtcgcc ttcggggtcg gttcggcgga
     9181 gccgtcggcg cgggtggggc gcctggccga gacgctggcg atcctcgagg cgctctggag
     9241 cggggaggcg gtcaccttcc ggggtgagca cttcaccctg acggacgctc gacagcagcc
     9301 gccccccacc ggtccgatcc cgttgacgat cggcggcagc ggccggcgga ccatggagct
     9361 ggtgcgccgc cacgccacct ggtggaacct gcctgtgcac cgggtcgacc gtctggagca
     9421 gctgcgaccc gccgccggtg acgcacgcgt gtcggtccag gtgatggtcg ccctgctcgg
     9481 agacggtgcc gaccggggtg cggtggccga gatggtggcc cgtcgctacg gcaccggggg
     9541 cctcgagtct cgggtggtcc tcggcaccgc agacgaagtc gccggccaca tggacgagct
     9601 cgccggtcgg ggagtggagc gggtccacgt ctggttcgcc gacttcgccc cgccgtcgac
     9661 gatcgacgcg ttcagcgccg tcatcggttg aacgctcaga gcgggaggac gtgcgacacg
     9721 aagtacagcg gcgccccgtc gcggtcgcgg acgaccgtcg cgatgaggtc ggcggagacc
     9781 ggcgatccgt ccggacgcag gtaccgcttg gtcatccggt agccgtcggc tcgaccctcg
     9841 aggcaggccc gcaggtgctc gacgtcggcg tcgaggtcct cggggtgggt gagctcctgg
     9901 aagcggcggc cggtcatctc ggcggtcgtc cgtccgagga aggccgcgaa gcggtcgctg
     9961 accgagcgca tggtcccgtc gaggtcgacg atcgcggtcg gcaccgcggc gctgaggacc
    10021 gcggtctcga accgggactc catcacccgt cgggaccggt gagcgggcgt gccgatggtg
    10081 tgggccggga tcgccgcctc gggcagaccg ttcgacggca gcggagccgg acgcccgaac
    10141 aggtagccct ggaagatcga gaccccgagg gtggcgaggc ggtcgaggtc ggcaggctcc
    10201 tccacccctt cggcgaccag ggtcgcaccc gagtgacggg cgagctcgct gatgccgccg
    10261 acgagagcct gacgggccgg gtcgcggtgg atcccctggc tgatggccag gtcgaccttc
    10321 acgatgtcgg gctcgagctc gaggatgtgg cggaagctgg cgaagcccgc accggcgtcg
    10381 tcgacggcga tgcgcacacc agcgccccgg aagcggtcca gggcgtgacg cagctgctcg
    10441 tagtcgccga cgcgggcgtg ctcggtcagc tccaggacga gccgggtcgg atcggccggg
    10501 accaactcgg acgggtcgac cgacaccagt gctgcgggcg acaggttgat gctcaggtag
    10561 gtgtccggcg gcaggttcgc cagcgcggag acggcggtgc gggcggcgtg cagctcgagg
    10621 gccaccccga ggccgaccgc ggccgcgtcc gcgaaccact ggtccgggcg ggccggctct
    10681 ccggggaacc gtgacagcgc ctcgtagccc gcgactcgac ggtgctccag gtcgacgatc
    10741 ggttggaacg cgatcgtgag gaggtcgtga tcgatcacgc gtccgatccg gtcgcggagc
    10801 cggcgtcggt cgtcgccgtc gatcgggacg tcgcggagca ggtcgctggc ggagtcggcc
    10861 agcatctgga ggaagtcgcg ggtccgctcg tcgacgtcgc gggggtccgt ggacaccgcg
    10921 gtcagcatgc cgatgacgct gccggtcgcc gccttgatcg gcacgctcac gaacgcacgg
    10981 atgcccagct gcgccgccat cggcaggtcc cgggtctcgg ggagcgcccg gacgtcgggg
    11041 acccagctgg cgatccgacc accgatgacc tgggcgcaga aggagtcctt cagcgggatc
    11101 gtcgagccct cgaggatcgt gctgtcgccg agggctccgt tgacgatggc cagcgacagg
    11161 acgtcgccgt tgatgcgcga cacccacacc atgtccatct cggtgcgcgt gcgggccacc
    11221 tcgagcaggc gacgcacgag atcttcgggc ccgtcgagat cggccaggtc ggtgtcggac
    11281 gatccgagcg acatcactgc gctatcggcg gcggctccgg ccacctgaag gtttcctgag
    11341 cacccggcgg gggtcgcggt ggaccgaggg tcaaccgcgg tggcggtcga ggaactccag
    11401 caccgcgctg ttgaacacct cgttgcggtc gccgaccacc atgtgcccgg ctccggccac
    11461 gtccgcgaac tcggcgtgcg gcacccgggc gaggaagtcc cgcgccccct cctcgctcag
    11521 gaggtcgctg gagcgacccc tgaccaacag ggtcgggacg cggagcgcgt ccgcggaccg
    11581 ttccaggagg tcggggccga cgatcgtggc ccgggtctcg tcagcagccc cgaacttgcc
    11641 ggacacgaac cgcggatccc agtgccagcg ccaccgaccg tcgggcccct ggcgcaggtt
    11701 cttggccagg ccgtcgaggt tggtgggacg gggacggtga gggttgtacg ccgcgatcac
    11761 gtcagccgcc tcgtcgagcg actcgaaccc gtcgaggttg ccggtcatga acgcgccgat
    11821 ccggtcggtg cccgccgact caaggcgatg ggcgacgtcg acgaggacca acgcccgagc
    11881 caggccgtcc tgcgggtccg cctcggcgac ggcgaccagg gccgacaggc cgccgagcga
    11941 cgctccgacc agcaccggag gtcgtccggt cgcggcggtg accgcaccgg cgaccgcggt
    12001 cgtgtcggcg gcgaaggcct ggagctggta gtcgccgtcg ccggaccact cgctgtcgcc
    12061 gtggccgcgc aggtccaccg tccaggcccg ccagccgtga tcggcgaggt agcgccaggt
    12121 gccggcccag gagtgtcggg tctggccgcc tccgtggagc agcacgacgg tcgggccgtc
    12181 gtcgggcccc gccacgtccg ccgccagcct gaggccgtcg ctgccgatca ggtcggtgcg
    12241 ggtcgcagcg accgcatcgt ccgttccatc ggtcccgtcc gcctcgcccg tcccgccgtg
    12301 cggggtcggt ggcgggccgg cggtcatgcc gtcccgtcgc ggatctcgac cagcgcggcg
    12361 gccaacgcgt cgggctggga caggaacggc gagtgcgacg acgccatctc caccaccacg
    12421 tccacgccga cctccgcgat catccgatcc tgctcggcgg gcacgatcgc ccggtcccgg
    12481 tcgcatcgca cgtaggcccg cggtaccgaa ccccagcgct cggcgctggt gtcgatggtg
    12541 gacatggccg ggccgatcgg ttcggggacc aggtgctcga tggccgtccg gacgtcctcg
    12601 ggtcggcagt cgccgtagaa caactcggcc gctgcgtcgg gccggatcga ggtcgcggag
    12661 ccgtcgggag ccatctcgat ggccgcgacc agcgcgctgt cgggatcgtg gcggtggagg
    12721 tcggcggggg tggcgcccga cggttgcagg agtgcgcaga ggtagacgag cccggcgatg
    12781 cgctcgggac gtcgttcggc gacggtgctg atggtggcgc ctcccaggct gtggcccacc
    12841 agggtgaccg gctcgtcgat gccgtcgagg acctggatca cccggtcggc gtagtcggaa
    12901 agggtcaacc ccgcgatcgg tgtgcggtcc tcgccgtggc ccgggaggtc gacggcgatg
    12961 ctgagcgcac cgaggccccg gagggagacc tggaggcggc tccagcacca ggccccctgc
    13021 caggccccgt gga
//
LOCUS       DAOQOD010000085        12902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1707_length_12902_cov_12.420721, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000085 DAOQOD010000000
VERSION     DAOQOD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_12902_cov_12.420721"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLZ72_09485"
     CDS             <1..539
                     /locus_tag="PLZ72_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HNA36568.1"
                     /translation="VAAAGGPPLAAGPGGHTQRLLTVVKSGSIQDVKAKPQDKVHVWP
                     HLLVIEFVAALVCTVFLLIFSWLVNAPLLELANVNQTPNPSKAPWYFLGLQELLTMFH
                     PMVAGVTIPGMGIFILILAPYMDKNPSNKPEDRKFAVSLMTLFLMFWAVIAIIGSFFR
                     GPGFNFTLPWIDGVFFEL"
     gene            557..1429
                     /locus_tag="PLZ72_09490"
     CDS             557..1429
                     /locus_tag="PLZ72_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNA36569.1"
                     /translation="MNGFFIVIAVVVLVVLAAVALLGAARRRDTDSATGSLSRESIKR
                     DRAARKEAAEMVPTGPSGQEVERSVALERRGGGDLEPVAAATPAVFVPPDPEAYDVSR
                     RQFLNRGVIAMMGLSIAAFGTAIVGFLWPSGSGGFGSKINMGKVTDLQADIGKNNGFL
                     YKPDGRLWITSYPASALPKAEQVYAPAVLNSLKAGLSVLYQKCPHLGCRVPSCNSSQW
                     FECPCHGSQYNQAGEKKGGPAPRGMDSFATEISGGNLIVNTGAVIQGPPIGTNTTGQE
                     AEGPHCIGAASGGH"
     gene            1594..2832
                     /locus_tag="PLZ72_09495"
     CDS             1594..2832
                     /locus_tag="PLZ72_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNA36570.1"
                     /translation="MILATSTQRAFGFVILAVVAIGFVVWFFANLRAGRDEVGSEIEL
                     APNRSPQLTDDELEGHRLNVALFSALGLLAIIAVALPLYWLAEPGRQKGAVEMYDELF
                     IERGLALYETGAQCVTCHGGAGVGGVTTFIINDQNGQFVGQVSWNAPALNNVLYRYDE
                     DQVRYILNYGRPGTPMAAWGAPGGGPLTTQQIDDIIAYLWSVQLKPEDMRKQVDDFVK
                     GIDPALLERMKEVRKANADVVPNGDVEAVNAKRLSRADELALGEILFNNQSLAQGSYS
                     CARCHVAGAPYGQAWQPFLRLQYGSFGPDLVGVENQATEQQHFNLVWNGMDEGKLYFS
                     RKQGNPQMPGFGINRNTGKEAEGVPDFGVEGMLTPEEVWAIVTYERNLSNDSTVKNSL
                     AGTIESPTFVNVPRDTAGSN"
     gene            2838..3839
                     /locus_tag="PLZ72_09500"
     CDS             2838..3839
                     /locus_tag="PLZ72_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36571.1"
                     /translation="MNILLAAIQFDPHIRGVLVVLVGVGVLIGSVYLLLATNTGVRNG
                     FLIAMAGLFGWMFCMGAIWWIYGIGLRGKDPSWHATEINYSRSGEPVHEVLAELPPTD
                     DLGNPQTLLSDFLADNPDVAKKIEQTEGEGFKATTFTKAVTAAPALKAGVDKELAPWR
                     ILPETDSRRGEAAASADAALVAGEAFGPDTATSSYTVKDVFFLGGKPGAEPETVKGER
                     STISQVWHRVQTVFQPKNPPLYAAVTVQQNTHQTVAPGEAVPPAQVNPDADTVTVLLQ
                     RNQGYRRLPSALFTIFCGTLFAVLTWMLHRRDKRAMKERAEWDPSKVPAVRDPETVT"
     gene            3842..4381
                     /gene="ndhC"
                     /locus_tag="PLZ72_09505"
     CDS             3842..4381
                     /gene="ndhC"
                     /locus_tag="PLZ72_09505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNA36572.1"
                     /translation="MGQYLPIVALLVLVAAFAGISLIAQRILVPPRPNAAKVAPYECG
                     ILDTSEPPERFPVRFYLIAMIFIVFDIEIIFFYPFTQVYGALGWFGLVAIGIFTVAVF
                     ESFLYLVSNGALDWGPPQHLRRSRLHVHADRTTANTVRRVGLEGRVDPDAGTDAVSSE
                     AHSDAETDVERDRETVGAH"
     gene            4381..5013
                     /locus_tag="PLZ72_09510"
     CDS             4381..5013
                     /locus_tag="PLZ72_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNA36573.1"
                     /translation="MGLVQNVRDDGFAGLEHNFLTGQLEDLVRWARRRSSWPATFGLA
                     CCAIEMMAAGGPHFDMARFGMEVFRASPRQADVMIVAGRVSQKMAPVLRTIYDQMMEP
                     KWVISMGVCASTGGMFNNYAIVQGVDQVVPVDVYAPGCPPGPETLLHAIVTLHGKIET
                     GEILRRREHNGGGAGLVVEQLDGQSAGGVLVPAEPVTASAAPGNFDIGTR"
     gene            5013..5561
                     /locus_tag="PLZ72_09515"
     CDS             5013..5561
                     /locus_tag="PLZ72_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNA36574.1"
                     /translation="MADQDATVVDDGPDAAEPAPEPTPEPELLFGVPVTTMRGQVVLH
                     PDRDGYVALVERLRSAGYWMCVDLCGVDYLGHPAGRWLPDGVTAERFEVVLNLLNHTE
                     HSRIRVRLQVPADDPVVPTISHLHPGVSNHERETFDLFGIAFDGHPDPSRILMPPGWV
                     GHPLRKDDAPARIPVQFKGAAS"
     gene            5558..6898
                     /locus_tag="PLZ72_09520"
     CDS             5558..6898
                     /locus_tag="PLZ72_09520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNA36575.1"
                     /translation="MSTTADRPDVVVETPSAATDLAALDSALERLRRDGATLEMSETE
                     AEEQAGYHVPAEEGVGERMLLNMGPQHPSTHGVLRLVLEMDGELITRSKPVIGYLHTG
                     MEKTGEQLTYLQGPTNVTRMDYAAPLFNELVFSLATEELLGIEVPERAQWIRTLMCEV
                     NRLSSHLLFMATNGMDLGAVGMMIYGWREREELLRFLELVTGLRMNHNYIRPGGVAVD
                     MPDGWREHLGPLLDVLPERLEEFDVLMTGQPIWRERLQGVGTITAEEAISLGATGPLL
                     RSTGYAWDLRRDMPYLRYDQVDFDVVVGTYGDSFDRYAIRLAEIRESIRIIRQCMDKM
                     PDGDYRIQDKKVTPPPRARIDESMEALIHHFKIFTEGFKVPEGEVYVAVENPRGELGC
                     YMVSDGSSNPVRMHIRGPSFVNLQSLPHLMKDSLIADTVAIVSSVDPIMGDVDR"
     gene            6898..7524
                     /gene="nuoE"
                     /locus_tag="PLZ72_09525"
     CDS             6898..7524
                     /gene="nuoE"
                     /locus_tag="PLZ72_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNA36576.1"
                     /translation="MARLTPDNEALARAIIARYPRPKSALIPLCHLAQEQDGWLTDEA
                     MVHIGELIGCTSAEVLGTASFYEMFKLHPVGRYCVNVCTNISCQLLGAEELLEHAEET
                     LGIRAGGTTEDGAITLEDVECIAACTEAPAIQVNYRYFHNVTVDDLDQIIAEARAGTL
                     SDRVPDHGTLGKVRQDVSADELANVTPPEQQSVRPWIARHQAAAEESA"
     gene            7524..8972
                     /locus_tag="PLZ72_09530"
     CDS             7524..8972
                     /locus_tag="PLZ72_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNA36577.1"
                     /translation="MEPRVSSRFEVVDGHTLAGYQDSGSYRGYDGIKAALAMSPDAVT
                     GMVKDATVLGRGGAGFPAGVKWGFCPPGVWPRYIVVNGDESEPGTYKDRVLMERDPHQ
                     LIEGCLIAAYAIGAAQVFLYVRGEMPHAQERIAAALNEAYAANLVGRNILGTDFSVDI
                     ILTWGAGAYIVGEETALIESLEGNRGMPRLKPPYFPAAKGLYMQPTIVNNVETLSNLP
                     WLFCHGVDEYRSIGTETSPGTRLVALSGHLNSPGVYEIPQGSTTYRELFESEQYGNGI
                     RGGRQLKMFIPGGASAPWFFPEQVDLPLEGRDVGAAGSMLGSGAIVVMDDTTDAVKAC
                     LRVVRFFARESCGKCTPCREGTTWEEKILQRILDGHGRPSDLDLLLDVADNISPGPYP
                     VAADPSQGLDAVPFPPRQTTICPLGPSSVAPITSALRRFRSEFEAKITRRNSIAVSTE
                     AFTAPADESVDELVGEAAGAEPLSGTGGRFDV"
     gene            8995..11484
                     /gene="nuoG"
                     /locus_tag="PLZ72_09535"
     CDS             8995..11484
                     /gene="nuoG"
                     /locus_tag="PLZ72_09535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNA36578.1"
                     /translation="MEITDPVTITVNGREVTAQKGELVIDACERNDAYIPRFCYHPRM
                     EPVGMCRMCLVEIDTGRGPALQPSCMIPVGADVTVTTDSPAVAKVQDGIIEYLLINHP
                     LDCPVCDKGGECPLQDQSMAYGPGESRWVEEKRHYEKPIELSATVLMDRERCILCDRC
                     TRFASEVTDDPLIHFIDRGANTQVNTFPGEPFNSYFSGNTVQICPVGALTARPYRFKA
                     RPWDLETTESTCTACSVGCRMTIDSSRDQVLRFNGVDSDPVNWSWLCDKGRFSFEAVA
                     SEDRLTEPLMRSGSGAGSTLAPARWNVALDAAAAAIRGALDSRGPAGVAVLGGSNLTN
                     ESAYAWARLAKGVIGTDQVDAQLGDGLPAEVFDLPGATIDDVCAPGGTVLYLGPDPKE
                     ELPVLFLRLKHAVLNDGVTLVQVTPTGTSLSRYAAASLHPRPGETAALVAALLGGAAG
                     SDVGGVPAAQLAAAADALAGGFSELRVVVGRANLAESPAVALAAAAAAFEARPTARFL
                     PVVRRGNTRGAVLAGLTPGRFPGGVTLDDGRDQWASVWPAVPEQAGGDADAILAAAAD
                     GKIDVLILLGADPLVDARDRSLARRALAATRTVIALDRFATESTAQAAIVLPVVGISE
                     CDGTTTNLEGRVSTLNARVTAPGTARADWMVAADLAELLGCTALADDGPAELWAELQA
                     NVAALDGLDAAALYSADDADGLVVGLDAGTFVAPAPPAVPAPNAYSSRLVVSRVLYDG
                     GSTVAHMPSSASLVRDGAVVLGPDDVATLDVQAGTRLTVSSPHGSVSGPLVVSAHVPK
                     GTVLIHHALSVDPSPLVSAADPVCDVRVEVA"
     gene            11484..12722
                     /gene="nuoH"
                     /locus_tag="PLZ72_09540"
     CDS             11484..12722
                     /gene="nuoH"
                     /locus_tag="PLZ72_09540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNA36579.1"
                     /translation="MDPLLSGDIGLAAVLIVLLKVVVAFTVLMLGVMAMVWFERKLIA
                     DMQNRIGPNRAGPFGILQTLADGLKLMFKEDLEPERSDPFVFKLAPFLALIPAFLVFA
                     VIPVAGNFSDGADGVVTIFGHETFMQVADPQMGILFVLAMSSVAVYGVMLAGWSSGSK
                     YPLLGSVRASAQMVSYEASLGLSVAAVLLFSGTLSTNGIVAEQSGGRWNLWMTALVPF
                     LIFIVSATAELNRPPFDLVEAEQELVGGFHTEYSSFRFALFFLAEFLNTVTMSAVIVT
                     LFLGGPSGPIPDWAPGWASDFVLPLIYFFAKLLIFLFMFVWFRATLPRFRYDQLMDLG
                     WKFLIPLSLGWFLILTSVKVAIDIGWNPVLTGAVTAAVVALGGLMLRSAVRTAAARHQ
                     AADPLTDQAILGSDAGGGGL"
     gene            12722..>12902
                     /locus_tag="PLZ72_09545"
     CDS             12722..>12902
                     /locus_tag="PLZ72_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNA36580.1"
                     /translation="MGYLDGFKVTFKKWAGTRTDSGRRVTMEYPEQVRPKPVRFHGRH
                     VLNRYEDGMEKCIGCE"
BASE COUNT         1903 a         4842 c         4195 g         1962 t
ORIGIN      
        1 cggtcgccgc cgcgggcgga ccgccgctgg ctgcgggccc cggcggtcac acccagcggt
       61 tgctgaccgt cgtcaagtcc ggttccatcc aggacgtcaa ggccaagccg caggacaagg
      121 tgcacgtgtg gccgcacctg ctggtcatcg agttcgtggc cgccctggtg tgcacggtct
      181 tcctgctgat cttctcgtgg ctggtcaacg cgccgctgct ggagctggcc aacgtcaacc
      241 agacgcccaa cccgtccaag gccccgtggt acttcctcgg tctgcaggag ctgctgacca
      301 tgttccaccc gatggtcgcc ggcgtgacca tcccgggcat gggcatcttc atcctgatcc
      361 tcgcccccta catggacaag aacccgtcga acaagcccga ggaccgcaag ttcgcggtct
      421 cgctcatgac gctgttcctc atgttctggg cggtgatcgc catcatcggg tccttcttcc
      481 gggggcccgg attcaacttc acgctgccgt ggatcgacgg cgtgttcttc gagctgtgat
      541 gtgaggtgag tcgaccatga acgggttctt catcgtcatc gccgtcgtcg tcctggtcgt
      601 gttggccgcc gtcgccctgc tgggtgcggc ccgccgccgc gacaccgact cggccaccgg
      661 ttcgctgtcg cgcgagtcga tcaagcgcga ccgcgccgcc cgcaaggagg cggcggagat
      721 ggtcccgacc ggaccgtcgg gccaggaggt cgagcgctcc gtcgccctcg agcgtcgtgg
      781 tgggggcgac ctcgagccgg tcgccgcggc caccccggcg gtgttcgtgc cgccggaccc
      841 cgaggcctac gacgtctcgc ggcgccagtt cctcaaccgt ggcgtcatcg ccatgatggg
      901 cctgtcgatc gccgcgttcg gcaccgcgat cgtcggcttc ctgtggccgt cgggctcggg
      961 cggcttcggc tccaagatca acatgggcaa ggtcaccgac ctgcaggccg acatcggcaa
     1021 gaacaacggc ttcctctaca agcccgacgg tcgcctctgg atcaccagct acccggcctc
     1081 cgcgctgccc aaggccgagc aggtctacgc cccggcggtg ctcaactcgc tcaaggccgg
     1141 cctgtcggtg ctctaccaga agtgcccgca cctgggctgc cgcgtcccga gctgcaacag
     1201 ctcgcagtgg ttcgagtgcc cgtgccacgg ctcgcagtac aaccaggccg gagagaagaa
     1261 gggcggcccc gccccccgcg gcatggacag cttcgccacc gagatctccg gcggcaacct
     1321 gatcgtcaac accggcgcgg tcatccaggg cccgccgatc ggcaccaaca ccaccggcca
     1381 ggaggccgag ggcccgcact gcatcggtgc ggccagcggc ggtcactgat cgccgtccag
     1441 caccgcagcg cacacaccag cagcgaacac accagcagcg caaccaccag cacagcacgc
     1501 caacagaagc ggcgacccga cccagccggg cgggccaccg agggagcagg gtgaccggca
     1561 ccgccggctc acgaggagac gtacgggaac cagatgatcc tcgcaacatc gacacagcgg
     1621 gccttcggct tcgtgatcct cgccgtcgtg gcgatcgggt tcgtcgtgtg gttcttcgcc
     1681 aacctgcgcg ccggccgcga cgaggtcgga tccgagatcg agctcgcccc caaccgcagc
     1741 ccgcagctca ccgacgacga gctcgagggg caccgcctca acgtcgccct gttctcggcc
     1801 ctgggcctgc tcgccatcat cgccgtcgcc ctcccgctgt actggctggc cgagccgggc
     1861 cgccagaagg gcgcggtcga gatgtacgac gagctgttca tcgagcgggg cctggccctc
     1921 tacgagaccg gcgcccagtg cgtgacctgc cacggcggcg ccggcgtcgg cggtgtcacc
     1981 acgttcatca tcaacgacca gaacggtcag ttcgtcgggc aggtgtcctg gaacgctccg
     2041 gcgctcaaca acgtcctgta ccgctacgac gaggaccagg tccgctacat cctcaactac
     2101 ggccgtccgg gaacgccgat ggcggcctgg ggtgcccccg gcggtggtcc gctcaccacc
     2161 cagcagatcg acgacatcat cgcctacctg tggtcggtgc agctcaagcc cgaggacatg
     2221 cgcaagcagg tcgacgactt cgtcaaggga atcgacccgg cgctgctcga gcgcatgaag
     2281 gaggtccgca aggccaacgc ggacgtcgtg cccaacggcg acgtcgaagc ggtcaacgcc
     2341 aagcgcctgt cccgcgccga tgagctcgcg ctgggcgaga tcctcttcaa caaccagtcg
     2401 ctggcccagg gctcgtactc ctgcgcccgc tgccacgtgg ccggcgcccc ctacggccag
     2461 gcctggcagc cgttcctgcg gctccagtac ggcagcttcg gccccgacct ggtcggggtg
     2521 gagaaccagg ccaccgagca gcagcacttc aacctggtct ggaacggcat ggacgagggc
     2581 aagctctact tctcccgcaa gcagggcaac ccgcagatgc cgggcttcgg catcaaccgc
     2641 aacaccggca aggaggccga gggcgtcccc gacttcggcg tcgagggcat gctcaccccc
     2701 gaagaggtgt gggcgatcgt cacctacgag cggaacctct ccaacgactc gacggtcaag
     2761 aactctctcg ccggcaccat cgagtcgccg acgttcgtga acgtcccccg tgacaccgcc
     2821 gggagcaact gagtccgatg aacatcctcc tcgccgccat ccagttcgac ccccacatcc
     2881 gcggcgtcct cgtcgtgctg gtgggcgtcg gcgtcctgat cggttccgtc tacctgctgc
     2941 tcgccaccaa caccggcgtg cgcaacggct tcctcatcgc catggccggc ctgttcggct
     3001 ggatgttctg catgggggcc atctggtgga tctacggcat cggcctgcgg ggcaaggacc
     3061 cgtcgtggca cgccaccgag atcaactatt cgcgaagcgg tgagccggtg cacgaggtgc
     3121 tcgccgagct gcctccgacc gacgacctgg gcaacccgca gacgctgctg tcggacttcc
     3181 tggccgacaa ccccgacgtc gccaagaaga tcgagcagac cgagggcgag ggcttcaagg
     3241 ccaccacctt caccaaggcg gtcaccgccg cgcccgcgct caaggcgggg gtggacaagg
     3301 agctcgcccc gtggcggatc ctgcccgaga ccgactcccg tcgtggtgag gccgccgcct
     3361 cggccgacgc cgcgctggtg gccggcgagg cgttcggccc cgacaccgcc acgtcgagct
     3421 acaccgtcaa ggacgtcttc ttcctgggcg gcaagcccgg agccgaaccc gagacggtca
     3481 agggcgagcg ctcgaccatc tcgcaggtgt ggcaccgggt ccagacggtg ttccagccca
     3541 agaacccgcc gctgtacgcc gcggtgaccg tgcagcagaa cacgcatcag accgtcgccc
     3601 cgggtgaggc ggtgccgccg gcgcaggtca atcccgacgc cgacacggtc acggtgctcc
     3661 tgcagcgcaa ccaggggtat cggcgcctgc cgtcggccct gttcacgatc ttctgcggga
     3721 ccctcttcgc ggtgctgacc tggatgctgc accgccgtga caagcgggcc atgaaggaac
     3781 gggccgagtg ggatccgtcc aaggtgccgg cggtgcgtga cccagagacg gtgacctgac
     3841 catgggccag tacctgccca tcgtggcgtt gctggtgctg gtcgctgcgt tcgccggtat
     3901 cagtctgatc gcccagcgca tcctggtgcc gccgcgaccc aacgcggcca aggtggcgcc
     3961 ctacgagtgc ggcatcctcg acacctccga gccgcccgag cgcttcccgg tgcgcttcta
     4021 cctgatcgcg atgatcttca tcgtcttcga catcgagatc atcttcttct acccgttcac
     4081 ccaggtgtac ggggcgctgg gctggttcgg cctcgtcgcc atcggcatct tcaccgtcgc
     4141 ggtgttcgag tcgttcctgt acctggtcag caacggggcg ctcgattggg gcccgccgca
     4201 gcacctgcgc cgctcccggc tgcacgtgca cgcggaccgc accaccgcca acacggtgcg
     4261 tcgcgtcggg ctggagggtc gcgtcgaccc cgacgccggg accgacgccg tgtcgtccga
     4321 ggcccactcc gacgccgaga ccgacgtcga gcgggaccga gagacggtcg gggcccactg
     4381 atgggcctcg tccagaacgt gcgcgacgac ggcttcgccg gcctggagca caacttcctg
     4441 accggccagc tcgaggacct ggtccgctgg gcccgccgcc gcagcagctg gcccgccacc
     4501 ttcggcctgg cctgctgcgc catcgagatg atggccgccg gcggcccgca cttcgacatg
     4561 gcccgcttcg gcatggaggt cttccgggcc tctccccgcc aggccgacgt gatgatcgtc
     4621 gccggccggg tctcgcagaa gatggccccg gttctgcgca ccatctacga ccagatgatg
     4681 gagcccaagt gggtcatctc catgggcgtg tgcgcgtcga cgggcggaat gttcaacaac
     4741 tacgcgatcg tccagggcgt cgaccaggtc gtccccgtcg acgtgtacgc ccccggttgt
     4801 ccgccgggcc ccgagacgct gctgcacgcc atcgtcaccc tgcacggcaa gatcgagacc
     4861 ggcgagatcc tccgccgtcg cgagcacaac ggcgggggcg ccggcctggt ggtcgagcag
     4921 ctcgacgggc agagcgcggg tggcgtcctc gtcccagccg agccggtgac ggcgtcggct
     4981 gcgcccggca acttcgacat cgggacccgt tgatggccga ccaagacgca accgtcgtcg
     5041 acgacggccc cgacgcggcc gagcccgcac cggaaccgac gcccgaaccc gagctgctgt
     5101 tcggcgtccc ggtcacgacc atgcgcggcc aggtcgtgct gcaccccgac cgcgacggct
     5161 acgtcgccct ggtcgagcgg ctgcgcagcg ccgggtactg gatgtgcgtc gacctgtgcg
     5221 gcgtcgacta cctggggcac cccgccgggc gttggctgcc cgacggcgtg accgccgagc
     5281 gcttcgaggt cgttctcaac ctgctgaacc acaccgagca ctcccgcatc cgggtgcgcc
     5341 tccaggtccc cgcggatgat ccggtggtgc cgacgatcag ccacctgcac cccggcgtgt
     5401 ccaaccacga gcgcgagacc ttcgacctgt tcggcatcgc cttcgacgga caccccgacc
     5461 cgagccgcat cctcatgccg ccgggctggg tcggtcaccc gctgcgcaag gacgacgctc
     5521 cggcccgcat cccggtccag ttcaaggggg cggcgtcgtg agcaccaccg cggaccggcc
     5581 cgacgtcgtg gtcgagaccc cgtcggccgc caccgacctg gccgcgctcg actccgcgct
     5641 cgaacggctc cgccgcgacg gcgccaccct cgagatgtcc gagaccgagg ccgaggagca
     5701 ggccggctac cacgtccccg ccgaggaggg cgtcggcgag cggatgctgc tcaacatggg
     5761 tccgcagcac ccctccaccc acggcgtgct gcgcctggtg ctcgagatgg acggcgagct
     5821 gatcacccgg tccaagccgg tcatcggcta cctgcacacc ggcatggaga agaccggcga
     5881 gcagctgacc tacctccagg gccccaccaa cgtcacccgc atggactacg cggcgccgtt
     5941 gttcaacgag ctcgtcttct cgctcgccac cgaggagctg ctcggcatcg aggtgccgga
     6001 gcgggcccag tggatccgca ccctcatgtg cgaggtcaac cggctcagct cgcacctgct
     6061 gttcatggcc accaacggca tggacctggg cgcggtcggg atgatgatct acggctggcg
     6121 cgagcgcgag gagctgctgc ggttcctcga gctggtcacc gggctgcgca tgaaccacaa
     6181 ctacatccgc cccggcggcg tggcggtgga catgcccgac ggttggcgcg agcacctcgg
     6241 cccgctcctc gacgtcctgc ccgagcggct cgaggagttc gacgtcctca tgaccggtca
     6301 gccgatctgg cgtgaacggc tccagggcgt cggcacgatc accgccgaag aggcgatcag
     6361 cctgggtgcc accggcccgc tgctgcgctc gaccggctac gcctgggacc tgcgccgcga
     6421 catgccgtac ctgcgctacg accaggtcga cttcgacgtc gtcgtcggca cctacggcga
     6481 cagcttcgac cgctacgcca tccgcctggc cgagatccgc gagtcgattc gcatcatccg
     6541 ccagtgcatg gacaagatgc ccgacggcga ctaccgcatc caggacaaga aggtcacccc
     6601 tccgccccgg gctcgcatcg acgagtccat ggaggcgctc atccaccact tcaagatctt
     6661 caccgagggc ttcaaggtgc ccgagggcga ggtctacgtg gcggtcgaga acccccgggg
     6721 cgagctgggc tgctacatgg tctcggacgg gtcgtcgaat ccggtccgca tgcacatccg
     6781 aggcccgagc ttcgtgaacc tccagtccct accgcacctg atgaaggact cgctgatcgc
     6841 cgacaccgtc gcgatcgtga gctcggtgga cccgatcatg ggcgacgtgg accgctgatg
     6901 gcccgcctca cccccgacaa cgaggcgctg gcccgggcga tcatcgcccg ctacccgcgc
     6961 cccaagtccg ctctcatccc gctgtgccac ctggcccagg agcaggacgg ctggctcacc
     7021 gacgaggcca tggtccacat cggcgagctg atcggctgca cctccgccga ggtgctcggc
     7081 acggccagct tctacgagat gttcaagctg cacccggtcg ggcgctactg cgtcaacgtg
     7141 tgcaccaaca tctcgtgtca gctgctcggc gccgaggagc tgctcgagca cgccgaggag
     7201 accctcggca tccgtgccgg cggcaccacc gaggacggtg cgatcaccct cgaggacgtc
     7261 gagtgcatcg ccgcgtgcac cgaggcgccc gccatccagg tcaactaccg ctacttccac
     7321 aacgtgaccg tggacgacct cgaccagatc atcgccgagg cccgcgccgg cacgctgtcg
     7381 gaccgggtgc ccgaccacgg cacgctcggc aaggtccgcc aggacgtgtc cgccgacgag
     7441 ctcgccaacg tgacgccgcc cgagcagcag agcgtccgtc cctggatcgc ccgccaccag
     7501 gccgcggccg aggagagcgc gtgatggaac cccgcgtctc gtcgcgcttc gaggtcgtcg
     7561 acggccacac cctggccggc taccaggact ccggctccta ccgcggctac gacggaatca
     7621 aggccgcgct ggccatgtcg cccgacgcgg tgaccggcat ggtcaaggac gccaccgtgc
     7681 tcggccgcgg cggcgccggc ttccccgccg gggtcaagtg gggcttctgc cctccggggg
     7741 tgtggccgcg ctacatcgtc gtcaacggcg acgagtccga gccgggcacc tacaaggacc
     7801 gcgtcctgat ggagcgcgac ccgcaccagc tgatcgaggg ctgcctgatc gcggcgtacg
     7861 ccatcggtgc cgcccaggtg ttcctctacg tccgtggcga gatgccccac gcccaggagc
     7921 gcatcgccgc cgcgctcaac gaggcgtacg ccgccaacct ggtcggccgc aacatcctcg
     7981 gcaccgactt cagcgtcgac atcatcctca cgtggggcgc gggggcctac atcgtgggcg
     8041 aggagacggc gctcatcgag tccctcgagg gcaaccgggg catgccgcgg ctcaagccgc
     8101 cgtacttccc ggcggccaag ggcctctaca tgcagccgac gatcgtcaac aacgtcgaga
     8161 cgctgtcgaa cctgccctgg ctgttctgcc acggcgtcga cgagtaccgc tcgatcggca
     8221 ccgagacctc gcccggcacc cgtctggtcg ccctgtcggg tcacctcaac tcgccgggcg
     8281 tctacgagat cccccagggc agcaccacct accgcgagct gttcgagtcc gagcagtacg
     8341 gcaacggcat ccgcggcggt cgccagctca agatgttcat ccccggcggc gcgtcggccc
     8401 cgtggttctt ccccgagcag gtcgacctgc ccctcgaggg gcgcgacgtc ggcgccgcgg
     8461 gctccatgtt gggctcgggc gccatcgtcg tcatggacga caccaccgac gcggtcaagg
     8521 cctgcctgcg ggtcgtgcgg ttcttcgccc gcgagtcctg cggcaagtgc acgccgtgcc
     8581 gagagggcac cacctgggag gagaagatcc tccagcgcat cctcgacggc cacggccgcc
     8641 cgtcggatct cgacctgctg ctcgacgtgg ccgacaacat cagccccggg ccgtacccgg
     8701 tggccgcgga cccctcccag ggtctcgacg ccgtgccgtt cccgccccgg cagaccacga
     8761 tctgcccgct cggtcccagc tcggtcgctc cgatcacctc ggcgctgcgg cgcttccgct
     8821 ccgagttcga ggccaagatc acgcgccgca actcgatcgc ggtgtccacc gaggcgttca
     8881 cggccccggc cgacgagagc gtcgacgagc tggtcggaga ggccgccggg gccgaaccgc
     8941 tgagcggcac gggaggccga ttcgatgtct gatcggcgcc tggagggtcg acgggtggag
     9001 atcaccgacc cggtgaccat caccgtcaac ggccgcgagg tcaccgccca gaagggcgag
     9061 ctggtgatcg acgcgtgtga gcgcaacgac gcctacatcc cgcgcttctg ctaccacccg
     9121 cgcatggagc cggtcggcat gtgccggatg tgcctggtcg agatcgacac cggtcgcggt
     9181 ccggcgctgc agccgtcgtg catgatcccc gtcggggccg acgtgacggt gaccaccgac
     9241 tcgccggcgg tggccaaggt gcaggacggc atcatcgagt acctgctcat caaccacccg
     9301 ctcgactgcc cggtctgcga caagggcggc gagtgcccgc tgcaggacca gtcgatggcc
     9361 tacggcccgg gcgagtcgcg ctgggtcgag gagaagcgcc actacgagaa gccgatcgag
     9421 ctgtcggcca cggtgctgat ggaccgggag cgctgcatcc tctgcgaccg ctgcacccgc
     9481 ttcgcctccg aggtcaccga cgacccgctg atccacttca tcgaccgcgg cgccaacacc
     9541 caggtcaaca cgttccccgg cgagccgttc aactcgtact tctcgggcaa caccgtgcag
     9601 atctgcccgg tcggcgcgct gaccgccagg ccgtaccggt tcaaggcccg cccgtgggac
     9661 ctcgagacca ccgagtcgac ctgcaccgcc tgttcggtcg gatgtcggat gaccatcgac
     9721 tcgagccgtg accaggtgct gcgcttcaac ggcgtcgact cggatccggt caactggtcc
     9781 tggctgtgcg acaagggccg cttctccttc gaggccgtcg cctccgagga ccgcctgacc
     9841 gaaccgctga tgcgctccgg ttccggcgcc ggctcgactc tggctccggc ccgctggaac
     9901 gtcgccctcg acgctgcggc cgccgccatc cgcggcgctc tcgacagccg cggcccggcc
     9961 ggcgtcgcgg tgctcggcgg ctccaacctg accaacgagt ccgcctacgc ctgggcccga
    10021 ctcgccaagg gcgtgatcgg caccgaccag gtcgacgccc agctcggcga cggcctgccc
    10081 gccgaggtgt tcgacctgcc cggcgccacc atcgacgacg tctgcgcccc cggcggcacc
    10141 gtgctctacc tgggacccga tcccaaggag gagctgccgg tcctgttcct gcgcctcaag
    10201 cacgccgtgc tcaacgacgg cgtcaccctg gtgcaggtca cccccaccgg caccagcctg
    10261 tcgcgctacg cggcggcctc gctgcacccc cgaccgggcg agaccgccgc gctcgtggcc
    10321 gccctgctgg gaggcgccgc cggctccgac gtcggaggag tgcccgccgc gcagttggcc
    10381 gccgcggccg acgctctcgc cggcgggttc tccgagctgc gcgtcgtggt cggtcgggcc
    10441 aaccttgccg agtcgcccgc cgtcgcgctc gccgccgcgg ccgccgcgtt cgaggcccgt
    10501 cccaccgccc ggttcctgcc cgtcgtccgc cgcggcaaca cccgcggcgc ggtgctggcc
    10561 gggctcaccc ccggccggtt ccccggcggc gtcaccctcg acgacggccg cgaccagtgg
    10621 gcttcggtgt ggcccgcggt ccccgagcag gccggcggtg acgccgatgc catcctggcc
    10681 gccgcggccg acggcaagat cgacgtgctg atcctgctcg gtgccgaccc gctcgtcgac
    10741 gcccgggacc gctcgctggc ccgacgggcc ctggccgcca cccgcaccgt gatcgccctg
    10801 gaccgcttcg ccaccgagtc gaccgcccag gcagccatcg tgctgcccgt cgtcggcatc
    10861 tccgagtgcg acggcaccac caccaacctc gaaggacgcg tcagcaccct gaacgcccgg
    10921 gtcaccgctc ccggcaccgc ccgcgccgac tggatggtcg ccgcggacct ggccgagctg
    10981 ctcggctgca ccgccctcgc cgatgacggc cccgccgagc tgtgggccga gctgcaggcc
    11041 aacgtcgcgg cgctcgacgg gctcgacgcc gccgcgctct acagcgcgga cgacgccgac
    11101 ggcctggtcg tcggcctcga cgccggcacg ttcgtggcac cggctccgcc cgcggtgccc
    11161 gcccccaacg cctactcgtc gcggctggtc gtgagccgcg tcctctacga cggcggttcc
    11221 accgtggccc acatgccctc gtccgcgtcg ctggtccgcg acggcgccgt cgtgttgggc
    11281 cccgacgacg tcgccaccct cgacgtgcag gccggcaccc ggctcacggt cagctcgccg
    11341 cacggttcgg tgagcggccc gttggtcgtc tcggcgcacg tccccaaggg gaccgtcctg
    11401 atccaccacg ccctgtcggt cgacccgtca ccgctggtgt cggccgccga cccggtgtgc
    11461 gacgtccggg tggaggtggc ctgatggacc cgctgctctc cggcgacatc ggcctggccg
    11521 ccgtcctcat cgtgctgttg aaggtcgtcg tcgccttcac ggtgctgatg ctcggcgtca
    11581 tggccatggt ctggttcgag cgcaagctca tcgccgacat gcagaaccgc atcggcccca
    11641 accgggccgg cccgttcggc atcctgcaga ccctcgcgga cggcctcaag ctgatgttca
    11701 aggaggacct ggagccggag cgctccgacc ccttcgtgtt caagctggcg ccgttcctcg
    11761 cgctgatccc cgcgttcctc gtcttcgcgg tgatcccggt ggccggcaac ttctccgacg
    11821 gcgccgacgg cgtggtcacc atcttcggcc acgagacgtt catgcaggtc gccgatccgc
    11881 agatgggcat cctgttcgtg ctcgccatga gctcggtcgc ggtctacggc gtcatgctcg
    11941 ccggctggtc ctcgggctcc aagtacccgc tgctcggctc ggtgcgcgcc tcggcgcaga
    12001 tggtctccta cgaggcgtcg ctcggcctgt cggtcgccgc ggtgctgctg ttcagcggca
    12061 ccctgtcgac caacggcatc gtcgccgagc agtccggcgg acggtggaac ctctggatga
    12121 cggccctggt gccgttcctc atcttcatcg tgtcggccac cgccgagctc aaccgcccgc
    12181 cgttcgacct ggtcgaggcc gagcaggagc tggtcggcgg cttccacacc gagtactcgt
    12241 cgttccgctt cgccctgttc ttcctggccg agttcctcaa cacggtcacc atgtcggcgg
    12301 tcatcgtcac cctgttcctg ggcgggccgt cgggtccgat cccggactgg gcgccgggct
    12361 gggcgtcgga cttcgtcctg ccgctcatct acttcttcgc caagctgctg atcttcctgt
    12421 tcatgttcgt gtggttccgg gccacgctgc cccgcttccg ctacgaccag ctgatggacc
    12481 tgggctggaa gttcctcatc ccgctgtcgc tcggctggtt cctgatcctg acctcggtca
    12541 aggtcgccat cgacatcggc tggaacccgg tcctcaccgg cgcggtgacc gcggcggtcg
    12601 tggcgctcgg cgggctgatg ctgcgcagcg cggtgcggac cgcggccgcc cgccaccagg
    12661 ccgccgaccc gctgaccgac caggcgatcc tcggctcgga cgccggcggg ggaggtctct
    12721 gatgggctac ctcgacggct tcaaggtcac gttcaagaag tgggccggca cgcgcaccga
    12781 ctccggtcgg cgcgtcacca tggagtaccc cgagcaggtg cgccccaagc cggtgcgctt
    12841 ccacggccgc cacgtcctca accgctacga ggacggcatg gagaagtgca tcggctgcga
    12901 gc
//
LOCUS       DAOQOD010000086        12839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1720_length_12839_cov_11.247966, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000086 DAOQOD010000000
VERSION     DAOQOD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_12839_cov_11.247966"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLZ72_09550"
     CDS             <1..197
                     /locus_tag="PLZ72_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36581.1"
                     /translation="LALLCRHHHGVIHRTGWQMTPNPQTDGASRDGADGDQTSFFTIT
                     TPDGAHLTTHHDRPPRPAPA"
     gene            complement(331..1191)
                     /gene="era"
                     /locus_tag="PLZ72_09555"
     CDS             complement(331..1191)
                     /gene="era"
                     /locus_tag="PLZ72_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNA36582.1"
                     /translation="MSTAAPDGFHSGFVTLVGRPNVGKSTLLNRILGQKVSIVSDKPQ
                     TTRHQIRGILTDDDTQIVFVDTPGIHRPRTLMGERLNDSASDALAGVDVVCLLFDATQ
                     KIGPGDRYIAERVPGDAIVVLNKVDRAPRERVLEQLALAAELDLASYFPVSARTGKGV
                     DALVEHLRSLMPPGPRWYPDGEVTDLGEGFRVAELVREQLLAVTHEELPHSIATRTTQ
                     YEWPRIRVEILVERESQKGMVIGKGGSVLKDVGTRVRRQLPDGAYLELFVKVDKDWQS
                     RPGSLERLGY"
     gene            complement(1188..2456)
                     /locus_tag="PLZ72_09560"
     CDS             complement(1188..2456)
                     /locus_tag="PLZ72_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA36583.1"
                     /translation="MNGTDLVLLVVILVLVLVAGVLAVAETAVTRMTRSRAAALAESD
                     PKRGRRVQRIVDNLERDLNAVYLAVNIVQTVQAALVGVLAGRLFGPLGVAIGIVLNVV
                     VLFTLAEAAPKTWALQHTDRAALMTAPMVELIGRVLRWPARSLIGVANIVLPGKGLKK
                     GPFVTEEEILALAEEAAEAAVIEESERELIESIIDFGDTVVREIMVPRPDVVSIGSDA
                     TVTDAVERSMESGRSRFPVYGESSDDVLGMVYVKDAYEAERTGGGGRGVLEVARPVLF
                     VPETKRVAELLTEMQARNTHVAVAVDEYGGTAGLVTLEDLLEELVGEIHDEFDEEGLD
                     VDEVADGIFLVNATLNVGELNERLDLSLPEGTWDSVGGLVFGTLGRVPAPGDAVDLDG
                     CRIVVDRVDGRRVSTVRVQRVGPAGEEDRS"
     gene            complement(2453..2983)
                     /gene="ybeY"
                     /locus_tag="PLZ72_09565"
     CDS             complement(2453..2983)
                     /gene="ybeY"
                     /locus_tag="PLZ72_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNA36584.1"
                     /translation="MGAEDRPAVARRDGREPGRAAEVAVDLDDLATLLGDVLTAEGVP
                     ATAEASLQLVDADEIAALNAEHMGSAGPTDVLSFPLDGADGPDDAASAGPSADRDWIV
                     GDVVVCPEVAAAQAADHAGSVADELALLVTHGGLHLCGWDHAEPAERDRMWARERELL
                     TALGRTPARDPWEGAG"
     gene            complement(2973..3938)
                     /locus_tag="PLZ72_09570"
     CDS             complement(2973..3938)
                     /locus_tag="PLZ72_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNA36585.1"
                     /translation="MEPARTTTAEPQTIVHVAGNHLMADLLGPRDELLDVVEDAFPRA
                     AITVRGNEISVHGDENDRVGRVFQELVLVLEQGQRIGPDVVRRTIDMVRADERPSEVF
                     THELLRTHRGGRVRPRTSGQKRYVDAIAGNIVTFGIGPAGTGKSWLAVAMGVQALQNK
                     DVERIVLTRPAVEAGERLGFLPGDLMAKVDPYLRPLYDALHDMVGTDTAQKLLERGAV
                     EVAPLAFMRGRTLNNSFIILDEAQNTTPEQMKMFLTRLGFGSKAVITGDVTQVDVPGG
                     RSGLGDLQRILDGIDDLAFVHLTGSDVVRHRIVADIVDAYAQRGG"
     gene            3958..4488
                     /locus_tag="PLZ72_09575"
     CDS             3958..4488
                     /locus_tag="PLZ72_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36586.1"
                     /translation="MASPEEPSRIPDLDAAAADLRIDLEVESRRRERWIRHRLAEEAT
                     LAGALAAAVGHDVAVQVLTGDRVAGVLAVVGNDVIEMRRHHGTTWVSIDAVTALEVPV
                     ALPAAGPALDGARMDEVLDGLAEERTEVVLTLDGGTSVRGVLVAVGTVATVDTGPGGR
                     TAYVPLSAIVSVTRPG"
     gene            complement(4553..4840)
                     /locus_tag="PLZ72_09580"
     CDS             complement(4553..4840)
                     /locus_tag="PLZ72_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA36587.1"
                     /translation="MGTADIEWLSTKEAARRLGVTPRTLYRFIDDGQLVAHRMGRVIR
                     VQEAEVADFIDRCRIQPGSLEALSDQALSDQALSDQAMSDGDPAEPADLGT"
     gene            5016..5693
                     /locus_tag="PLZ72_09585"
     CDS             5016..5693
                     /locus_tag="PLZ72_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNA36588.1"
                     /translation="MTATPPMTTPPPGGRARRLIPSGRATVGAALVVVAAAGVLSTHR
                     AATAAPDERVVVATRSVPSGTVLTADDLGTVAMDLPEGMAAVTADRAEELIGTTSRHD
                     LREMDLVRRSDVARADSDPAAGSTIVPVEVPRTRALVDSVHSGSRVEVLATDPDGTGT
                     RVLATDVLVVGVDDGGSDDGIGVSDRVGYRLAVLDVETAASVVDAAVRSELTIVMSTG
                     RGGPTDG"
     gene            5686..6975
                     /locus_tag="PLZ72_09590"
     CDS             5686..6975
                     /locus_tag="PLZ72_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36589.1"
                     /translation="MADRYVVIGLARSRSRWFGEVARWANAAAAPVDFVKCLTSEEAR
                     AVLGAGRRVSALLLDTELSRFDRDLVADAARDGVPTLLVGSADRRDWEALGCATRLDA
                     DFTRDELVDALDRFARAVAGDDEPTSARVDLSSDPVAGTLIGVLGAGGAGTSTIAMAL
                     AQAWAATGADTVLVDGCRRGDLAMYHDVGDVIPGLPELVELHRGDEADPDEIRSLEFE
                     TRRGYRLVLGLRRARDWAGMRSRAVAAAVHGLQRTHEVSVLDLDDDLETEAETGSADI
                     EDRHALSLAVARRADAVVVVAGADLKGIHDASRLAHDLHRCGTPAERIVVVVNRAPRS
                     TVARAQLARSVRALTDDLGDDGIGAALTVRRHRLEATHHDATALPEGIGCAVLSATRG
                     VIGAGAARPTADPVRIRVGELGTRLESEPVTFHGGRP"
     gene            6972..8273
                     /locus_tag="PLZ72_09595"
     CDS             6972..8273
                     /locus_tag="PLZ72_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNA36590.1"
                     /translation="MSTISDSDLRSPLEEIEQLVQSRANALALDVDTADGRSRLRELI
                     SNAVEDWADDHARGVRPHGLPDPQGVVDRAFRNLARYGPLTDLLADPDVWEIMVNAPD
                     EIFVKRHSGGNRFHHEVFHDDDHVTRTLTKLLDDSATSHRKLDPAEGLQDAQLDDGAR
                     LHIVHSDLSRGGHLMVNIRKFTGVAITRLNELVERGSMTRGAARFLAEAVRARLSIVF
                     AGAPGAGKTTLLSCCAAELDPSLRVVIAEEVFEADVELANVAGMQTRPPRTDRPGIDL
                     RRLVAGFLRMAPDVAIVGEVRDREALPLLLTLSSGVTGYTTIHAGSARQGLTRLRFLA
                     QLSDSSRDLPLSALNNLVSDSVDLVVHTERGPDGPRLTGIVAVEDLVSGPEAVHFTVT
                     DVFGRGIDGSLDWSGDVPVRAAERMAARGIDLRALLEAAGR"
     gene            8270..9142
                     /locus_tag="PLZ72_09600"
     CDS             8270..9142
                     /locus_tag="PLZ72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA36591.1"
                     /translation="MTSLILAGLAATGVYLLVTPRPARRGSTRERFDVRAVADRAELA
                     LQRAGLDGVSPTQFAATVLGVAVVAAALASLVIGPGIGAALVGLLAGSIPVTSWRSRR
                     SAARRAAREHWPRLIEELRVLTGPMGRPIPQALLEVGSRGPAALRPAFAAAQREWNLT
                     TDFERTVTVLKDRLADPTADATCETLLVVHEVGGELDRRLASLAEDRRRDLNDRRDAQ
                     ARLAGARTARLFVILVPLGMAVAGLNVGDGREAYRSTIGQILVVAAILMVGLCWWWAA
                     RLMRLPVDERVFDR"
     gene            9139..10038
                     /locus_tag="PLZ72_09605"
     CDS             9139..10038
                     /locus_tag="PLZ72_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA36592.1"
                     /translation="MSGAELLLLLIGGTGVALVLDQFRWAHRRSMARRLTPYGSAGRP
                     GPRIAAVSSPAAVLMPLVEQSLDRVTRALGIDDDLATRLARADVDVDPARFRTRQALH
                     AVLGLAGGAALALLVRPGSFVSVLTVVGLPLLWVLLDEQRISNRIRHRSHVLQVELPV
                     VAEQLGILIDAGSSLPSALARIARRGRGAAAEDLQRVVLRIRSGLGEAEALSEWADRS
                     DTGAVRRLVSVLSLHREAADLGQLISAEAQAIRAESHRELVERIERRGQLVWIPVTVA
                     TLVPGLLLLAVPFVSALSQVTGA"
     gene            10126..10338
                     /locus_tag="PLZ72_09610"
     CDS             10126..10338
                     /locus_tag="PLZ72_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36593.1"
                     /translation="MSSSASNTTSTTSTTARRRRVRSEIAEGVISTAIAVLIVAFLGI
                     GLWKGFDVMMNSATEKTQNQVEQIGN"
     gene            10452..10757
                     /locus_tag="PLZ72_09615"
     CDS             10452..10757
                     /locus_tag="PLZ72_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36594.1"
                     /translation="MCVGLWTRSTVEAVAQDAARDLAATPEGGLDRARIDEVLDRARS
                     RLGSAGARTDLRLEGVDGSVRLRVRHPGVSLVPRMLSGHGAFGSIDEVIVVGREDTR"
     gene            10754..11221
                     /locus_tag="PLZ72_09620"
     CDS             10754..11221
                     /locus_tag="PLZ72_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36595.1"
                     /translation="MSTSRGERGAVAGLEGVLFAVLILVTGTLVVTNSWSVLQVRRSL
                     DGAAREYIRGYTQAADPTAAQRDGRIALFDVLDGEGVRAGTVSIDGPDPSVFGPCATA
                     TVTLSSTVPALLLPFIGRVASTTVRVTHTELVDAHREMTVGRSYDPEATACAR"
     gene            11211..11624
                     /locus_tag="PLZ72_09625"
     CDS             11211..11624
                     /locus_tag="PLZ72_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36596.1"
                     /translation="MRAERATALVMVPAVALVFIVLAAIALDLTAVAGVQRSAERTLA
                     AAVDDAAGMIDGRAHQADGSIRIDRARAEQLVRARIGAEELPGRVVGLTVDVTATTVD
                     ATMRIETPHIFLRAVPGLADSTLSSPIHLRARLRD"
     gene            11641..>12839
                     /locus_tag="PLZ72_09630"
     CDS             11641..>12839
                     /locus_tag="PLZ72_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNA36597.1"
                     /translation="MDHLETLEPHALHRLDHYRAPGQGMTLTRDRVGDESVLRKSARG
                     GQRAALRREAEVLRALGGHGVVRLIEIVDGADVTELVVLDTGGPSLAAALADPATDSR
                     SALRLLADACDAVSRLHEQGWAHGDLRADHVLLTRRHHIRLCSLGDAVSLGTEPDGGR
                     ADRVALLRIVDDWTRSAGTDGSPMSSRIRSRVLARRTHRLPDDPDPRLLGRILRRTAA
                     TGLAVPSRDRIRLPLPVRSGRRVRRVAVPAVTIILVAGIAWFALPSHGPDPGPGGSDS
                     GGGTAHTTQMSTTTTVALGAAPPAPETAAASGCRAVERSRPDVDGDGCGDDVSIEHDV
                     VVAGGHRYRVGRVGDLAAIGDWDCDGTSTIAVLRPEDGTVHVFDRWALDGRPAQAATW
                     GRVDGAAT"
BASE COUNT         1779 a         4745 c         4492 g         1823 t
ORIGIN      
        1 acctcgcgtt gctgtgccgc caccaccacg gcgtcatcca ccgcaccgga tggcagatga
       61 cccccaaccc ccagaccgac ggagcctcaa gagacggcgc tgacggcgat cagaccagct
      121 tcttcaccat caccaccccc gacggcgccc acctcaccac ccaccacgac cggccaccca
      181 gacccgcacc cgcctgagcg cgggaccggc accggaccaa cccacgctcc acccacccag
      241 cccgggtcag acccacaccc accgaccccc gcgccccgac gcgggcacga ccgtgcacgg
      301 ggcacacccg cgtcggtcgc gctcgatccg tcagtagccg agtcgttcga gggagccggg
      361 tcgggactgc cagtccttgt ccaccttcac gaacagctcc aggtacgcgc cgtcgggcag
      421 ctgacgtcgg acgcgggtgc cgacatcctt gagcaccgac ccgcccttgc cgatcaccat
      481 gcctttctgc gactcgcgct cgaccaggat ctcgacccgg atgcgcggcc actcgtactg
      541 ggtggtgcgc gtggcgatcg agtgcgggag ctcctcgtgg gtgacggcca ggagctgctc
      601 gcggaccagc tcggccaccc ggaacccctc ccccaggtcg gtcacctctc cgtcggggta
      661 ccagcggggg ccgggcggca tgagcgagcg caggtgctcg accagagcgt cgacgccctt
      721 gccggtgcgg gccgagaccg ggaagtacga cgccaggtcg agctcggcgg ccagggcgag
      781 ctgctcgagc acgcgttccc ggggagcgcg gtcgaccttg ttgagcacaa cgatggcgtc
      841 gccgggcacc cgctcggcga tgtagcggtc gccgggaccg atcttctggg tggcgtcgaa
      901 cagcaggcac accacgtcga ccccggccag ggcgtcggag gccgagtcgt tcagccgctc
      961 ccccatcagg gtgcggggtc ggtggatgcc gggcgtgtcg acgaacacga tctgggtgtc
     1021 gtcgtcggtg aggatgcccc ggatctggtg acgggtcgtc tgcggcttgt ccgagacgat
     1081 cgacaccttc tggcccagga tgcggttgag cagcgtggac ttgcccacgt tggggcgacc
     1141 gacgagcgtc acgaaaccgg agtggaaccc gtcaggtgct gcggtgctca cgatcggtcc
     1201 tcctcgcctg ccggcccgac gcgctgcacc cggaccgtgc tcacgcgccg accgtcgacg
     1261 cggtccacga cgatccgaca gccgtcgagg tcgaccgcgt cgccgggagc cggcacccgg
     1321 ccgagggtcc cgaagaccag tccgccgacg gagtcccagg tcccctcggg cagcgacagg
     1381 tcgagccgct cgttcaactc ccccacgttc agggtggcgt tgaccaggaa gatgccgtcg
     1441 gcgacctcgt cgacatcgag gccctcctcg tcgaactcgt cgtggatctc accgaccagc
     1501 tcctcgagca ggtcctcgag ggtgaccagg ccggcggtgc cgccgtactc gtcgaccgcg
     1561 acggcgacgt gggtgttgcg ggcctgcatc tcggtcagca gctcggcgac ccgcttggtc
     1621 tccggcacga aaaggaccgg ccgggccacc tcgaggacgc cgcgcccgcc gcccccggtt
     1681 cgttcggcct cgtacgcgtc cttgacgtac accatgccga gcacgtcgtc ggagctctcc
     1741 ccgtacaccg ggaagcggct gcggccggat tccatcgacc gctccaccgc gtcggtcacg
     1801 gtcgcgtcgg agccgatcga gaccacgtcg gggcgcggca ccatgatctc ccgtacgacc
     1861 gtgtcgccga agtcgatgat cgactcgatc agctcccgtt cggactcctc gatcaccgcg
     1921 gcctcggccg cctcctcggc gagggcgagg atctcctcct cggtgacgaa cgggcccttc
     1981 ttgaggccct tgcccggaag gacgatgttc gcgacgccga tcagcgatcg cgccggccat
     2041 cgcagcaccc ggccgatgag ctcgaccatc ggagcggtca tcagcgcggc acggtctgtg
     2101 tgctgcaacg cccaggtctt gggcgcggcc tcggcgaggg tgaacaggac gacgacgttc
     2161 aacacgatgc cgatcgcgac gcccaacggc ccgaagagcc gtccggccag cacaccgacc
     2221 agggcggcct gcaccgtctg gacgatgttg acggccaggt agacggcgtt gaggtcgcgc
     2281 tcgaggttgt cgacgatgcg ttgcacccgc cggccccgct tgggatccga ctcggccagg
     2341 gcggcggcgc gggaccgggt catgcgggtg accgcggtct cggcgacggc gagcacgccg
     2401 gcgaccagca ccagcaccag gatcacgacc aacaggacca ggtcggtgcc gttcacccgg
     2461 ccccctccca cggatcgcgt gccggtgtgc gaccgagcgc cgtcagcagc tcccgctcgc
     2521 gggcccacat ccggtcgcgt tcggcgggtt cggcgtggtc ccagccgcac aggtggaggc
     2581 ccccgtgggt gacgagcagg gccagctcgt cggccaccga tccggcatgg tcagccgctt
     2641 gggccgccgc gacctcggga cagacgacga cgtcgccgac gatccagtcc cggtcggccg
     2701 acgggccggc cgacgcggcg tcgtcgggtc cgtcggctcc gtcgagcggg aacgacagca
     2761 cgtcggtcgg accggccgaa cccatgtgct cggcgttgag ggcggcgatc tcatcggcgt
     2821 cgaccagttg caatgacgcc tcggcggtcg cggggacgcc ctcggccgtc aggacgtcgc
     2881 cgagcagggt ggcgagatcg tccaagtcga cggccacctc ggcggcccga cccggctcac
     2941 ggccgtcgcg gcgggcgacc gccggacggt cctcagcccc cacgttgggc gtaggcgtcg
     3001 acgatgtcgg ccacgatgcg gtgtcgcacg acgtcggagc cggtcaggtg cacgaaggcg
     3061 aggtcgtcga tgccgtcgag gatccgttgc aggtccccca gtccggaccg gcccccggga
     3121 acgtcgacct gcgtgacgtc gccggtgatc acggccttgg agccgaaccc caaccgggtg
     3181 aggaacatct tcatctgctc cggcgtggtg ttctgggcct cgtccaggat gatgaagctg
     3241 ttgttgaggg tgcggccccg catgaacgcc agcggggcga cctcgacggc tccccgctcg
     3301 aggagcttct gggccgtgtc ggtgccgacc atgtcgtgca gcgcgtcgta gaggggacgc
     3361 aggtacgggt cgaccttggc catcaggtca ccgggcagga acccgagacg ctcgccggcc
     3421 tcgaccgccg ggcgggtcaa cacgatgcgc tcgacgtcct tgttctgcag cgcctggacg
     3481 cccatcgcga cggcgagcca ggacttgccc gtgcccgccg ggccgatgcc gaaggtgacg
     3541 atgttgccgg cgatggcgtc gacgtagcgc ttctgtccgc tggtgcgggg ccggacccgg
     3601 ccgccacggt gggttcgcag cagctcgtgg gtgaacacct cggacggtcg ttcgtcggcg
     3661 cggaccatgt cgatggtgcg ccgcacgacg tcgggaccga tgcgctggcc ctgctcgagg
     3721 accagcacga gctcctggaa caccctcccc acccggtcgt tctcgtcgcc gtggaccgag
     3781 atctcgttgc cccgcaccgt gatggcagcg cgggggaagg cgtcctcgac cacgtcgagg
     3841 agctcgtcgc ggggtccgag gaggtcggcc atgaggtggt tgcccgcgac atggacgatc
     3901 gtctgtggtt cggcggtcgt cgtgcgagcg ggttccatgg aagggcacag tgtaccgatg
     3961 gcctcccccg aggaaccgag ccggattccc gacctcgatg cggccgcggc cgacctgcgc
     4021 atcgatctgg aggtcgagtc ccggcggcgg gagcgctgga tccggcaccg actggcagag
     4081 gaggccaccc tggccggggc gttggcggcc gccgtcggcc acgacgtcgc ggtccaggtc
     4141 ctgaccggtg accgcgtggc cggtgttctc gccgtggtcg gaaacgatgt gatcgagatg
     4201 cgacgccacc acggcaccac ctgggtgtcg atcgacgcgg tcaccgctct ggaggtcccc
     4261 gtcgccctgc ccgccgcggg tccggctctc gacggcgccc gcatggacga ggtgctcgac
     4321 ggcctggccg aggagcgcac cgaggtggtg ctcaccctcg acggcggcac gtcggtgcgc
     4381 ggcgtgctgg tcgcggtggg aaccgtggcc accgtcgaca ccggtccggg tggtcgcacc
     4441 gcctacgtcc cgctgtcggc catcgtgtcg gtgacacgac ccggctgacg acgcggggcg
     4501 accagggggg cgggaaccgg gcccgaccct gccccgggcc cacccggcgc ggtcaggttc
     4561 cgaggtctgc cggctccgcc gggtctccgt ctgacatggc ctgatcggac agagcctgat
     4621 cggacagggc ctgatcggac agggcctcga gcgagccggg ctggatgcga cagcggtcga
     4681 tgaagtcggc gacctccgcc tcctggaccc ggatcacccg acccatgcgg tgcgcgacga
     4741 gctgaccgtc gtcgatgaag cggtacagcg tccgcggggt gacccccaga cgccgagccg
     4801 cctccttcgt gctgagccat tcgatgtccg ccgtgcccat gccgacacgg tacgccccga
     4861 ccgaacctcc ggcaaccagc gggcagtcga ccgtgtctac gcccacgttg atggctttga
     4921 tgatctttct ggtcatttcc tccctcttga ctttgttcca actcgtcccg taacactctg
     4981 cacatgccgc agagccggga cggaaggacg acgacgtgac cgcaacaccg ccgatgacaa
     5041 cgccgccacc aggcgggcgg gcacgtcgcc tgatccccag cggccgcgcc acggtcggcg
     5101 ccgccctggt cgtcgtcgcc gcggccggcg tcctgtccac ccatcgagcc gccaccgcag
     5161 cacccgacga gcgggtggtg gtcgcgacca ggtcggtccc ctcggggacg gtgttgaccg
     5221 ccgatgacct cgggaccgtc gccatggacc tgcccgaagg gatggccgcc gtgacggcgg
     5281 accgggccga ggagctgatc gggacgacca gccgacacga cctccgggag atggacctgg
     5341 tgcgccgcag cgacgtcgcc cgggccgact ccgacccggc ggcgggatcg acgatcgtcc
     5401 ccgtcgaggt gccgcggacc cgggcgctgg tcgactcggt ccacagcggc agccgcgtcg
     5461 aggtgctcgc caccgacccc gacggcaccg gcacgagagt cctcgccacc gatgtgctcg
     5521 tggtcggcgt cgacgacggc ggttccgacg acgggatcgg ggtgtcggat cgggtcgggt
     5581 accgcctcgc cgtcctcgat gtcgagaccg ctgcgtcggt cgtcgacgca gcggtgcgct
     5641 cggagctgac catcgtcatg tcgaccggcc ggggaggacc gaccgatggc tgaccgctac
     5701 gtcgtgatcg gcctcgcccg gtcccggagc cggtggttcg gcgaggtcgc ccgatgggcc
     5761 aatgccgcgg ccgccccggt ggacttcgtc aagtgcctca ccagcgaaga ggcccgcgcc
     5821 gtgctcgggg ccggtcgccg ggtctcggcc ctgctcctgg acaccgagct gtcccgattc
     5881 gaccgcgacc tcgtcgcgga cgccgcccgc gacggcgtcc cgacgctcct cgtcggatcc
     5941 gccgaccgtc gggactggga ggcgctcggc tgcgccaccc gcctggatgc ggacttcacc
     6001 cgcgacgagc tggtggacgc cctcgaccgc ttcgcccgtg ccgtcgccgg cgacgacgag
     6061 ccgacctcgg cccgggtcga cctgtcctcg gacccggttg ccggaaccct gatcggcgtc
     6121 ctcggtgcgg gcggcgccgg cacgtcgacc atcgcgatgg cgttggccca ggcgtgggcc
     6181 gcaaccggag ccgacaccgt gctcgtcgac gggtgccggc ggggtgacct ggccatgtac
     6241 cacgacgtcg gcgacgtcat ccccggcctg cccgagctcg tggagctcca ccgcggcgac
     6301 gaggccgacc ccgacgagat ccgctcgctc gagttcgaga cccgccgcgg gtaccggctg
     6361 gtgcttggtc tacgtcgagc ccgcgactgg gcgggaatgc ggtcgcgggc cgtcgcggcc
     6421 gccgtccacg ggttgcagag gacccacgag gtctcggtcc tcgacctcga cgacgacctg
     6481 gagaccgagg ccgagaccgg ctcggccgac atcgaggacc gccacgcgct gtccctggcc
     6541 gtcgcccgcc gagccgacgc cgtcgtggtc gtcgcgggag ccgacctgaa gggcatccac
     6601 gacgcctcgc gtctcgccca cgacctccac cgctgcggga cgcccgccga gcgcatcgtc
     6661 gtggtggtga accgcgctcc ccgatccacg gtcgcccggg cccagctcgc ccgcagcgtc
     6721 cgggccctca ccgacgacct cggggacgac ggcatcggtg cggccctgac cgtgcgtcgc
     6781 caccggctcg aggccaccca ccacgacgcc accgccctcc cggaggggat cgggtgcgcc
     6841 gtgctgagcg cgacccgcgg ggtcatcggt gccggagcgg cccgaccgac agccgatccg
     6901 gtgcgcatcc gggttggaga gctcggcacc cgcctggagt cggagccggt cacgttccac
     6961 ggaggtcggc catgagcacg atctccgact ccgacctccg ctcaccgctg gaggagatcg
     7021 aacagctggt gcagtcgagg gccaacgccc tggccctcga cgtcgacacc gccgacggcc
     7081 ggtcccgcct gcgggagctg atcagcaacg cggtcgagga ctgggccgac gaccacgccc
     7141 gcggcgtccg gccccacggc ctgcccgacc cccagggagt cgtcgaccgc gccttccgca
     7201 acctggcccg ctacggcccg ctcaccgacc tgctggcgga ccccgacgtg tgggagatca
     7261 tggtcaacgc ccccgacgag atcttcgtca agcggcattc gggcgggaac cgcttccacc
     7321 acgaggtctt ccacgacgac gaccacgtca cccggacgct gaccaagctg ctcgacgact
     7381 cggccacatc gcaccgcaag ctcgatcccg cggagggact tcaggacgcg cagctcgacg
     7441 acggcgcccg gctgcacatc gtgcacagcg atctcagccg aggtggccac ctcatggtga
     7501 acatccggaa gttcaccggc gtcgccatca cccgcctcaa cgagctcgtg gagcgcggtt
     7561 cgatgacccg gggagcggcc cgcttcctcg cagaggcggt acgggcccgg ctcagcatcg
     7621 tcttcgccgg agcgcccggc gccggcaaga ccacgttgct gtcctgctgc gcggcggagc
     7681 tcgacccgtc cctgcgagtg gtcatcgccg aagaggtgtt cgaagccgac gtcgagctgg
     7741 ccaacgtcgc cgggatgcag acccgccctc cgcggacgga ccgccccggg atcgacctcc
     7801 gtcggctggt cgcgggcttc ctgcgcatgg cgcccgatgt cgccatcgtc ggcgaggtcc
     7861 gcgaccgaga ggcgctgccc ctgctgttga ccctgtcgtc gggcgtgacc ggatacacca
     7921 ccatccacgc cggatcggct cgccaggggt tgacccggct gcggttcctg gcccagctgt
     7981 cggactcctc acgggatctg ccgctgagcg ctctcaacaa cctggtcagc gactcggtcg
     8041 acctggtggt gcacaccgag cgaggcccgg acgggccacg tctcaccggg atcgtcgcgg
     8101 tcgaggacct cgtctcaggg cccgaggccg tccacttcac ggtcaccgac gtgttcggcc
     8161 gcgggatcga cggatcgctg gactggtccg gtgacgtgcc ggtccgagcg gccgagcgca
     8221 tggccgcccg aggcatcgac ctgcgcgccc tgctcgaggc ggccggacga tgacgtcgct
     8281 gatcctcgcc gggctcgcag cgacgggcgt ctacctgctc gtcacacccc gccccgcccg
     8341 tcgaggctcg acgagggagc ggttcgacgt ccgtgccgtg gcggaccgag cggagctcgc
     8401 cctgcaacgc gcaggcctcg acggcgtctc ccccacccag ttcgcggcga ccgtgctggg
     8461 cgtcgcggtg gtcgccgccg ccctcgcgtc gttggtgatc gggcccggca tcggggccgc
     8521 cctcgtcgga ctcctggccg gctcgatccc ggtcacctcc tggcgcagcc ggcgcagcgc
     8581 cgcccgacgg gcggcccggg agcactggcc ccggctcatc gaggagctcc gggtgctgac
     8641 cggcccgatg ggccgaccca tcccccaggc cctgctcgag gtcgggtcgc gaggcccggc
     8701 cgcgctccgt cccgccttcg ctgctgctca acgcgagtgg aacctgacga ccgacttcga
     8761 acggaccgtg acggtgttga aggaccgtct cgcggatccc accgcggatg ccacctgcga
     8821 gacgctgctc gtcgtccacg aggtgggcgg tgagctcgac cgacggctgg cgagcctcgc
     8881 cgaggacagg cgtcgcgacc tgaacgaccg ccgcgacgcc caggccaggt tggccggagc
     8941 gcgcacggcc cggttgttcg tcatcctcgt cccgttgggg atggccgtcg ccgggctcaa
     9001 cgtcggagac ggccgggagg cgtaccgcag caccatcggc cagatcctgg tggtcgccgc
     9061 catcctcatg gtcggcctct gttggtggtg ggcggcccga ctgatgcgcc tcccggtcga
     9121 cgagcgggtg ttcgaccgat gagcggcgcc gagttgctgc tcctgctgat cgggggcacc
     9181 ggtgtggccc tggtcctcga ccagttccgc tgggcccatc gacgatccat ggcgcgacgg
     9241 ctgactccgt acgggtcggc gggacgtccc ggcccacgga tcgccgccgt gtcctcccct
     9301 gccgcggtgc tcatgcctct cgtggagcag tcactcgacc gggtcacccg ggccctgggg
     9361 atcgatgacg acctggccac gaggctggcc cgggccgacg tggatgtgga tccggcccgg
     9421 ttccggaccc gacaggcgct gcacgccgtc ctcggtctgg ccggcggagc ggcactcgca
     9481 cttctcgtcc gcccgggttc gttcgtgtcg gtgctgaccg tggtcgggct cccgctcctg
     9541 tgggtgctgc tcgacgagca gcggatctcg aatcggatcc gccaccgatc ccatgtcctg
     9601 caggtcgagc ttcccgtggt ggccgagcag ctggggatcc tgatcgacgc cggatcgtcg
     9661 ttgccgtccg cactggcccg gatcgcccgg aggggacggg gtgccgcggc cgaagacctg
     9721 caacgggtgg tcctccgcat ccgcagcggg ctcggcgagg ccgaggccct gtcggagtgg
     9781 gccgaccggt cggacaccgg ggcggtccgg cgactcgtgt cggtgctgtc gctgcaccgg
     9841 gaggcggccg acctcgggca gctgatctcg gccgaggccc aggcgatccg agccgaatcc
     9901 caccgcgagc tggtggagcg gatcgagagg cggggccagc tcgtgtggat ccccgtcacc
     9961 gtggcgacac tcgtgccggg actcctcctc ctggccgttc cgttcgtgtc ggccctgtca
    10021 caggtgaccg gcgcatgagc acgcaccagc agccacaccg acagcgccca cttcgacaac
    10081 agcacccact tcgacaacag cacacacacc gacagggaga cagccatgag cagttcagcc
    10141 agcaacacga ccagcacgac cagcacgacc gcacggcggc gacgggtccg atccgagatc
    10201 gccgaggggg tcatctccac ggcgatcgcg gtcctgatcg tggcgttcct cggaatcggc
    10261 ctgtggaagg ggttcgacgt gatgatgaac agcgccacgg agaagaccca gaaccaggtc
    10321 gaacagatcg gcaactgagg ccggaggcct ggcccggctc ggtcgccggg gtcggcgagc
    10381 gtggctccgg acttctcagc acccttctcg gggtctccgc cctggtcgtc gccctgggtc
    10441 tcgccaccaa cgtgtgcgtc ggcctgtgga cccgatcgac ggtcgaagcc gtcgcccagg
    10501 acgccgcccg cgacctggcc gccaccccgg agggcggcct ggaccgagcc cggatcgacg
    10561 aggtcctcga ccgggcccgc tcgcgcctcg gatcggccgg ggcgcgcacc gacctccgac
    10621 tcgagggggt cgacgggtcc gtccgtctcc gggtccgcca cccgggcgtc agcctggtgc
    10681 cacggatgct cagcggtcat ggagcgttcg gctcgatcga cgaggtgatc gtggtcgggc
    10741 gggaggacac ccggtgagca cctcacgcgg tgagcgcgga gccgtcgccg gcctcgaggg
    10801 agtgctgttc gccgtcctga tcctggtgac ggggaccctg gtggtgacca actcctggtc
    10861 ggtgctgcag gtccgtcggt ccctcgacgg agcggcacgc gagtacatcc gcgggtacac
    10921 ccaggctgcc gaccccaccg ccgcgcagcg tgatggccgg atcgctctgt tcgacgtgct
    10981 cgacggtgag ggggtgcggg ccggcaccgt gtcgatcgac ggacccgatc cgtcggtctt
    11041 cggcccgtgt gccaccgcga cggtcacgct gtcgagcacg gttccggcgt tgctgctccc
    11101 gttcatcggc cgagtcgcgt cgacgacggt ccgggtgacc cacaccgagc tcgtcgacgc
    11161 ccaccgtgag atgaccgtcg gtcgctccta cgacccggag gcgaccgcct gtgcgcgctg
    11221 aacgggccac ggccctggtc atggtgcccg cggtggcact ggtgttcatc gtcctggccg
    11281 cgatcgctct ggacctgacc gcggtcgccg gcgtccaacg atcggctgag cggaccctcg
    11341 ccgccgcggt cgacgatgcg gccgggatga tcgacgggcg cgcacaccag gccgacggct
    11401 cgatccgcat cgaccgggcc cgagcggaac aactcgtccg ggcacggatc ggggccgagg
    11461 agctgccggg gcgggtcgtc gggctgaccg tcgacgtcac cgcgaccacg gtggacgcca
    11521 ccatgcggat cgagaccccg cacatcttcc tccgcgccgt tcccggcctg gccgacagca
    11581 ccctgtcctc ccccatccac ctgcgggccc gcctgcggga ctgacccgag acgagacgtg
    11641 atggaccacc tcgagacgct ggagccccac gccctgcacc gactcgacca ctaccgcgcc
    11701 cccggccagg ggatgaccct gacccgcgac cgggtcggcg acgagtcggt gctgcggaag
    11761 tccgctcggg gcggacagcg cgccgctctg cgtcgagagg ccgaggtgct gcgggccctc
    11821 ggcggccatg gtgtggtgcg cctgatcgag atcgtcgacg gcgcggacgt caccgagctc
    11881 gtcgtcctcg acaccggtgg gccgtccctg gccgcggcgc tggccgaccc ggccaccgac
    11941 agccgttccg cgctccgact gctcgcggat gcctgcgacg cggtgagtcg actgcacgaa
    12001 cagggatggg cgcacggcga cctgcgcgcc gaccacgtgc tgttgacccg gcgacaccac
    12061 atccggttgt gctcgctcgg cgatgccgtg tccctcggca cagagcccga cggcgggcgc
    12121 gccgatcggg tcgctctcct gcgcatcgtc gacgactgga cccgctcggc cgggacggac
    12181 ggctccccga tgtcgagtcg catccgctcc cgcgtgctgg cgcggcgcac ccatcgcctg
    12241 cccgacgatc ccgacccgcg actgctgggc cggatcctcc gccgcaccgc cgccaccggt
    12301 ctggccgttc catcccggga ccgcatccgc ctcccactcc ccgtccgatc cggtcgccgg
    12361 gtacgacggg tcgcggttcc tgcggtcacg atcatcctcg tcgccgggat cgcgtggttc
    12421 gcgctcccct cccatggccc ggatccgggg cccggcggca gcgacagcgg gggcggcacg
    12481 gcacacacca cccagatgtc caccaccacg acggtcgccc tcggcgccgc gccacccgcg
    12541 cccgagacgg ccgccgcttc cggctgtcgc gccgtcgagc ggtcccgacc cgacgtcgac
    12601 ggcgacggct gcggcgacga cgtcagcatc gagcacgacg tcgtcgtcgc cggcggccac
    12661 cgctaccggg tgggacgagt cggtgacctg gcggcgatcg gcgactggga ctgcgacggc
    12721 acctcgacca tcgccgttct gcgacctgag gacgggacgg tccacgtgtt cgaccgctgg
    12781 gcgctcgacg gccggccggc gcaggccgcg acgtggggac gcgtcgacgg cgcggcgac
//
LOCUS       DAOQOD010000087        12481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1782_length_12481_cov_6.871077, whole genome shotgun sequence.
ACCESSION   DAOQOD010000087 DAOQOD010000000
VERSION     DAOQOD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_12481_cov_6.871077"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLZ72_09635"
     CDS             complement(<1..420)
                     /locus_tag="PLZ72_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36598.1"
                     /translation="MAGVVGGDASAVVVVDDVRVAVTVTTAGGGAVDSVNGQTGVVVL
                     AAADVGADPAGTAAGLVDDLSGVSNAATARTNLGLGALATLSSVSSAEIVDGTIVNAD
                     IGAGAAIALSKLAVDPLARANHTGTQAQSTVTNLVSDL"
     gene            complement(741..944)
                     /locus_tag="PLZ72_09640"
     CDS             complement(741..944)
                     /locus_tag="PLZ72_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36599.1"
                     /translation="MSRYSPKNILEQEPAVISGAVIVAVNALALFGVVTVSAEQLAGL
                     NAGLAALLALFTRQTVTPNAKVR"
     gene            complement(956..1942)
                     /locus_tag="PLZ72_09645"
     CDS             complement(956..1942)
                     /locus_tag="PLZ72_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36600.1"
                     /translation="MPVTVRDQLGGRIPPGWTPAPCVELDNEPWDGFDVPWRTDISDW
                     PVDQARTANLARSDVQVPVDVSTPGQVWDGSVGGIPVQDCRNVATTTVWDLGRPVTWE
                     WFSPRWPTVQVPLPALVRREGDPMAAWDRHWCGIDVDRRRLHEAIQLDSSPWNRVRTI
                     GHTDWTAGWPGGGPGLATWDLSRPWNNPGQPRGVVAAAVPLVPHLIRADELARGVIDH
                     ALFLVLADYAGEHTGYARGSDGTARNHPCRAGERLRLPAADVAFYRPGTPERVVADAL
                     HRFGAIVGDRSDHSGSATSKPGRLSCAQDPRINTGWTGLGLRLSDLEVVHQP"
     gene            complement(1942..2733)
                     /locus_tag="PLZ72_09650"
     CDS             complement(1942..2733)
                     /locus_tag="PLZ72_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNA36601.1"
                     /translation="MKVPGIPFDPGKHPQGPLAPTAIVLHRTYGAFGKDGYRSAYAVG
                     KNGRAGVGIGFHFLVGKNAGQVVQFYDTAVEAAHAKGANSWSVGIEFDGVNEDVLTDW
                     QVAQAAWIIAAVCDAHRIPRIYTTAGIRRRVAGCLPHALVPGSDHTDAVTVADWTRIA
                     AHWTGAATPATTEEDAMTPQQRDEVVGRLDEAIGLLRSIHPAVAQIQTGVMDPVAGVR
                     TMVVQLLGQAHTAADPTQVAQAIAQALPDGLARQVADELAARLAA"
     gene            complement(2730..3023)
                     /locus_tag="PLZ72_09655"
     CDS             complement(2730..3023)
                     /locus_tag="PLZ72_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36602.1"
                     /translation="MAADPIVYLAGAALVLIGSLAAVRGTQRGKEADTAIALSAEMRE
                     WADELLQAEQTCRTELEKVRAEVDRLHGLLVETRDELDSTRAEVRRLRAEVQR"
     gene            complement(3023..3412)
                     /locus_tag="PLZ72_09660"
     CDS             complement(3023..3412)
                     /locus_tag="PLZ72_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36603.1"
                     /translation="MTHRSRPVGHTVRVFCAAAAGLGLWQVGWIADQSSRLGELLPAP
                     WSPTFWIAAWSLVAVAGAVAAVTGADVATRVALVALATTSGLGIVTALALDLSMGRQF
                     WQVGQAALIVVVCFALLVSPLRDDTVH"
     gene            complement(3409..3939)
                     /locus_tag="PLZ72_09665"
     CDS             complement(3409..3939)
                     /locus_tag="PLZ72_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36604.1"
                     /translation="MTTPFVDPQSLHNPATGTSPPASWGDAVRDGVVFCATPPSCRAN
                     RTATQSIADITLTAVSFTAADSWDTDSFHSTVSNQSRLTVPSGLGGKYLIVASANWAN
                     NSTGWRDLSIRANGSTNLAAARVGTGTANPYAISTITTVEQLAAGTYIEVTIWQNSGA
                     ALDITAANVTILQVSL"
     gene            complement(3950..5983)
                     /locus_tag="PLZ72_09670"
     CDS             complement(3950..5983)
                     /locus_tag="PLZ72_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36605.1"
                     /translation="MSFDRVAVTIEMAFGFGPGSSPAAGDWVDLSDWVDLDSSSPAIV
                     ATSGRDTVRAIRPGSLSLTLDNSTGRFNPRNPSGPYYGDLTNGTPVRVRVTHNSTTTT
                     RWSGFVDSGWPQQLTAQLPVVTVTAQDVLGVLAEGAAPDTAFDAQVAAGVAPTHWWRP
                     GEGGWTDKMTSITSRHTGGFESFSAGQTPFGPVVDGDAQTWGQTDADGYGVVVDPGSK
                     IDLTSRQIISMWVQLPGPEDRQLHYLGSPAPLWLLTQCINAPGAPGDVMCLIVQIAAT
                     GALVFVDDRHTGAASPKANLWGSVAGPGGVDRAWLGADGTVHHVAVALNTAAQEPLLW
                     VDGVPVDLMLGSSLSGARAATLTDLFIGQGNDPSAVPYQGVIDHVMVFEDFGSGAFAA
                     PGANPTEDAFVAALYEAGRVAWAGDSLDERLDHVVTAMGLAADTGTFDPSGITTLQGY
                     RAGDVAGLLQTVEDTEQGRIWVDRDGDLRFSKRTWAWDDTVSTTVQMTFSDDPTLLAG
                     GAQEMAETGTVIVDDPLAVTNVAAVNSTYGRQQTAENTASVAAFGRRNAVQLSGLLHP
                     TDKQSLAIAEWIVASQGTATPQVRQISFRVENNPTVLAPFARAVEPGWLVRVHKKAAS
                     GQDLDVTAHVIGVTHTWTFTGWMVTLTLDATRAGWSWFKWGTSTWGGSAAWSF"
     gene            complement(5980..8244)
                     /locus_tag="PLZ72_09675"
     CDS             complement(5980..8244)
                     /locus_tag="PLZ72_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36606.1"
                     /translation="MSNTRKLTIEILGNAKDATRAMLDVRSDSDKMGESLMKVGGIAT
                     GAGVALVGGLLWAAKESENAERQVRKLDNSIANSSQTFKENGKALKDLAADIQSKTAA
                     DADAIVGAQALMVQFGLTEAQVTSLTPLVVDLSQKMGVDLDTAAKAALKAVDGSSTSL
                     KRMGINVDEAAFKLDPFNATVDTLRSTVGGFAEQEGATFSGQMERMKNQLGDIVEGVG
                     GGAIEVFGGITDKVSGLVGATSAANPELANTVGKLGAIGGASLIAVGGLSTAAGWLLK
                     MRDRFTSVSGEGDNAVRSLNGIGKAAAGIAAAGAAVGVVEGVFAVLNQSSGTAARAAA
                     GLDKLKLALADAGSGAGDVVDAFGEMVGAEQDTLRLQNLWQEFGAEVSIVGTGVKADI
                     EQVQRTFDTLGDTDGPEKQLAVLDALEKQTQSLDHSSDQYKTNMEFIERNRAAVEQHV
                     AAVKADRRATDEMTGSTEDGTEATEDATSALQDYADQLRANTDPLFAVIDAQDRVADA
                     KKRVDEAVTSGDLDAWRTATMAVTQAQFDLEGRMGALSTAVANGKVNIDQAKGAVDRL
                     ASSGVITAQQAGVMKSQFDGVAWTTAVAGNRMDETRSKAWWLGQEIGKPRSMHIDSSQ
                     AEQAIGTVQNRLAALRGMMMMQTWGLGGLIAPGRRAMGGPVTKGLPYIVGERGWELFV
                     PDASGTVITHDDAVRAASSGVAPTGGAASSPVTVNVTFAGPVAADSISWVAEQVETAV
                     AKGVRFPRLARSMA"
     gene            complement(8390..8707)
                     /locus_tag="PLZ72_09680"
     CDS             complement(8390..8707)
                     /locus_tag="PLZ72_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36607.1"
                     /translation="MTIEQVGVIPAFRIRYQGEEVTVDHRTFTLGERRRSRRALVELT
                     EADDLSPDFVDQLAALAWVVLTRSQPELSISDLFEQVDLGDIADGEVLTGETPEDESD
                     PEA"
     gene            complement(8726..9193)
                     /locus_tag="PLZ72_09685"
     CDS             complement(8726..9193)
                     /locus_tag="PLZ72_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36608.1"
                     /translation="MPVNRSATVEVRGLKELRQKLKDIDLEKDLKKVHHRVADLVAVD
                     ARQVMAGLPVGGMAGRAASTIKASRSAVGARINLGSASVPFALGVEFGAHRNVQRRRG
                     SHSMLGWNQFQEWRGAGDGAGYAIFPTIRDNRERIMAAYMDEIDRLVRPAFPD"
     gene            complement(9198..10181)
                     /locus_tag="PLZ72_09690"
     CDS             complement(9198..10181)
                     /locus_tag="PLZ72_09690"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNA36609.1"
                     /translation="MSGFLGQLGVKDEVIYGTLVTPDRFFEFLSEGLAAETGRVESPG
                     IRAGTRVLRSDRRVPYINGVTGSIELDVLSLGWSWWLKHMLGTVATTGPAETSVYTHT
                     GTVGDLTGDFFTLQVGVPQIGGGAITPKTVTGCKVTSWELACKAGETLKFKADIDGQT
                     LDHTTGLATASYPAAVEPITFIRGVVTVGGTQVDVAEFSVKVDNTLKTDRRYLRSNAL
                     KKEPVESGTRKVSVELKCDFENLTHQNRVLSTTAAGAQAQVVLVCEGLTTIGTTLKPK
                     VTITVPVVMFDGDTPAVGGPDAVDQSLKGVGLYDGTSSPISVAYQVASAAP"
     gene            complement(10178..10372)
                     /locus_tag="PLZ72_09695"
     CDS             complement(10178..10372)
                     /locus_tag="PLZ72_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36610.1"
                     /translation="MKVRYIGPFTDGVEIAAGGRIWQAEHGVDVDIPDDVAAGLVTQT
                     DIWEQSKPPAKPATKKGEQP"
     gene            complement(10390..10830)
                     /locus_tag="PLZ72_09700"
     CDS             complement(10390..10830)
                     /locus_tag="PLZ72_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36611.1"
                     /translation="MAQSAVPAAVRALHDLLVDGCPWPDREPDIGFGVPAELGREMVI
                     LGPVDGTEDWAQLGARRRDCDFEIGVTVIVRWPGHDALEACDRAYALFAVIEDQLRNP
                     DNIALAHSAGVLWNEIAHPASTPTVEDEGYGHVIQSAVRFRART"
     gene            complement(10830..11687)
                     /locus_tag="PLZ72_09705"
     CDS             complement(10830..11687)
                     /locus_tag="PLZ72_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36612.1"
                     /translation="MIVADEQILAGSSATITGRFRDQDGDLVDPTGPVTVTVTRSDGT
                     AVLTAAATTTPSGTVGVRQAALNATQTTTLDELTATWGDGTQEVTTRIEVVAAYYAST
                     ADIRASDPALTDTAKYPSAAVVAARRLVETEFEDICGVAFVPRYRLAPMRASGATRVV
                     LDDPMLRTVRYADGLTAPQLAAVAASADGVAELPSGTWPSTPWHIGYEHGFDRPPADV
                     LGAFLTRVRDVLNRSNRGVPDRTSSFTSGENGGTFALIVPGQRGSKTGIPDVDVVLDR
                     WSMRTPGIA"
     gene            complement(11687..11911)
                     /locus_tag="PLZ72_09710"
     CDS             complement(11687..11911)
                     /locus_tag="PLZ72_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36613.1"
                     /translation="MTTDDHLRGLREELRGYEASGRTDRAAQVRAEIDRLSSAEVAAP
                     VTEAAVADDPAVETADKPATGRGRGRKKAD"
     gene            complement(11985..>12481)
                     /locus_tag="PLZ72_09715"
     CDS             complement(11985..>12481)
                     /locus_tag="PLZ72_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNA36614.1"
                     /translation="ASPTVAEAWPKLADAIQRINSARFMPGNVIYMHPRRWGWITAAV
                     DTSGRPLFEFSTTPPQSVIGLGQAAEYGQVVGTLQGLPVITDASIPTTLGGGTEDVIC
                     VARSFDILYWEDDLLQFTFEQVPATAPGQVRLAAGRFSLFTPGRYPTSISTISGTGLA
                     APTF"
BASE COUNT         1789 a         4186 c         4521 g         1985 t
ORIGIN      
        1 caaatcactg acgaggttcg tcaccgtcga ctgtgcctgt gtccccgtgt gattcgccct
       61 cgccaacgga tcaaccgcca acttgctcag agcaatcgcc gcacccgccc cgatgtcagc
      121 gttgacgatc gtgccatcga cgatctccgc cgacgacacc gacgacaacg tcgcaagcgc
      181 acccaacccc agattcgtcc gggccgtcgc agcgttcgac acaccggaga ggtcgtcgac
      241 gaggcccgcc gccgtaccgg ccgggtcagc gccgacatcc gcggcggcga ggacgacgac
      301 cccggtctga ccgttcaccg agtcgaccgc accaccgccg gcggtcgtga cggtcacggc
      361 gacgcgcacg tcgtcgacga cgacgaccgc cgaagcgtca ccgccgacca ccccggccac
      421 cccaggctgg gcgacatcga gcggaccggc gagcgtcgtc accccatcga cggtgagcgt
      481 ccacgtccac ccgcggccgc tcaactgtcc ggtgaccgcg tcggcgaacg cgacggtcac
      541 ctcgttgttc gacgccccgc cgaccgtcgc ggtcccggcg tgcgacgtcg accagttcgc
      601 gcacgccgcc gcgatggtcg accccgacag gtcgaccggc gacccggcgg cggtcgtgaa
      661 cgtcgcgacg agtgtcgccg ggtcgcccgc ggtgacaagc caccgcagga accccggacg
      721 ggacgcaacc gatgaggtca tcagcgcacc ttcgcgttgg gtgtgacggt ctggcgggtg
      781 aacagggcga gcagcgcggc gagcccggcg ttgagcccgg cgagctgttc ggcggagacg
      841 gtgacgacac cgaacagggc cagcgcgttg accgcgacga tgacggcacc ggagatcacg
      901 gccggttcct gctcgaggat gttcttcggg gagtagcgcg acatcagctt gtccttcagg
      961 gttggtggac gacttcgagg tccgacaggc gcaggccgag acctgtccag ccggtgttga
     1021 tgcgaggatc ctgggcgcag gacaggcgac ccggtttcga ggtggcggag ccggagtggt
     1081 cggatcggtc accgacgatg gcgccaaacc ggtggagggc gtcggcgacg actcgttcgg
     1141 gggtgccggg ccgatagaac gcgacatccg cggcgggaag ccggagccgc tcgccggccc
     1201 gacacggatg gttgcgggcg gtgccgtcgg acccgcgggc gtagccggtg tgttcgccgg
     1261 cgtagtcggc gaggaccagg aacagggcgt ggtcgatgac gccgcgggcg agttcgtcgg
     1321 cgcggatgag gtgcgggacg agcggcaccg cagcggcgac cacaccgcgg ggttggccgg
     1381 ggttgttcca cggccgggag aggtcccagg tcgcgaggcc gggcccgccg ccgggccagc
     1441 cggcggtcca gtcggtgtgg ccgatggtgc gcacccggtt ccacggcgac gagtcgagct
     1501 ggatcgcttc gtggaggcgg cgtcggtcga cgtcgatgcc gcaccagtgg cggtcccatg
     1561 cggccatcgg gtcgccttcg cggcgcacca gcgccgggag tggcacctgg acggtcggcc
     1621 aacgggggct gaaccactcc caggtgacgg gccgtccgag gtcccagacg gtggtggtgg
     1681 cgacgttgcg gcagtcctgg acggggatgc ctccgacgga gccgtcccac acctggccgg
     1741 gggtcgacac gtcgacgggg acctgcacgt cgctgcgggc gaggttcgcg gtgcgggcct
     1801 ggtcgacggg ccagtcggag atgtcggtgc gccatgggac gtcgaacccg tcccacggtt
     1861 cgttgtcgag ttcgacgcac ggtgcgggtg tccagccggg ggggatccgg ccgccgagtt
     1921 ggtcgcggac ggtgacgggc atcaggcggc gagccgggct gcgagctcat cggcgacctg
     1981 gcgggccagg ccgtcgggca gcgcctgcgc gattgcctgg gcgacctgcg tcgggtcggc
     2041 cgcggtgtgc gcctggccga gcagctgcac caccatcgtg cgcacccccg caacaggatc
     2101 catcaccccg gtctggatct gtgcgacggc ggggtggatg gagcgcagca ggccgatggc
     2161 ttcgtcgagc cggccgacga cctcgtcgcg ttgctggggg gtcatggcgt cctcctctgt
     2221 ggtggcgggg gtggctgcac cggtccagtg cgcggcgatc cgggtccagt cggcgacggt
     2281 gacggcgtcg gtgtggtcgg agccggggac gagggcgtga ggcaggcatc cggcgacgcg
     2341 tcggcggatg cctgcggtcg tgtagatgcg ggggatgcgg tgcgcgtcgc agaccgcggc
     2401 gatgatccac gctgcctggg cgacctgcca gtcggtgagg acgtcctcgt tgacgccgtc
     2461 gaactcgatg ccgacgctcc acgagttggc gcccttggcg tgcgcggcct cgacggcggt
     2521 gtcgtagaac tggacgacct gcccggcgtt cttgccgacc aggaagtgga acccgatgcc
     2581 gacgccggct cggccgttct tgccgaccgc gtacgcggac cggtagccgt ccttgccgaa
     2641 cgccccgtac gtgcggtgca gaacgatcgc cgtcggagcg agcggcccct gcgggtgctt
     2701 gccgggatcg aacgggatgc cggggacctt caccgctgca cctccgctct cagcctgcgc
     2761 acttcggcgc gggtcgagtc gagctcgtct cgggtttcga ccagtaggcc gtgcagccga
     2821 tcgacctcgg cccgcacctt ctcgagctcg gtgcgacacg tctgctccgc ctggagcagt
     2881 tcgtcagccc actcacgcat ctcggccgac aaggcgatgg cggtgtcggc ctccttgccg
     2941 cgttgagtgc cgcgcaccgc ggcgaggctg ccgatgagca cgagggcggc gccggccagg
     3001 tagacgatcg ggtccgctgc catcagtgga cggtgtcgtc gcgcagcgga gagacgagca
     3061 gggcgaagca gacgacgacg atgagggcgg cctggccgac ctgccagaac tgtcggccca
     3121 tcgacaggtc gagagcgagg gcggtgacga tgccgagccc ggatgtggtc gcgagagcga
     3181 cgagcgcgac gcgggtcgcg acatcggcac cggtcaccgc tgcgacagcg cccgccaccg
     3241 cgacgaggct ccacgccgcg atccagaacg tcggtgacca cggtgcgggg aggagctcgc
     3301 cgagccggct cgactggtcg gcgatccacc cgacctgcca gaggccgagg cccgcggccg
     3361 cggcgcagaa cacgcggacg gtgtggccga cgggccgcga ccggtgggtc atagcgacac
     3421 ctgcaggatc gtgacgttcg cggcggtgat gtccagggcg gcgccggagt tctgccagat
     3481 ggtgacctcg atgtaggtgc cggcggcgag ctgctcgacc gtggtgatgg tgctgatcgc
     3541 gtacgggttc gcggtgccgg ttccgacacg tgcggcggcg aggttggtgg atccgttcgc
     3601 tcggatcgac aggtcccgcc accccgtcga gttgttcgcc cagttcgccg acgcaacgat
     3661 caggtacttg ccgccgaggc cggacggcac ggtgagccgc gactggttcg acaccgtcga
     3721 gtggaagctg tcggtgtccc acgagtccgc cgcggtgaac gacacggcgg tcagggtgat
     3781 gtcggcgatc gactgggttg ccgtccggtt cgctcgacac gacggcggcg tcgcgcagaa
     3841 cacgacgccg tcacgcactg cgtcgcccca cgacgccggc ggtgacgtgc ctgtggccgg
     3901 gttgtgcagc gattgggggt cgacgaacgg tgtggtcatc tcgatctcct cagaacgacc
     3961 aggcggccga gccgccccac gtggaggtcc cccacttgaa ccacgaccag ccggcgcggg
     4021 tggcgtcgag ggtgagggtc accatccagc cggtgaacgt ccaggtgtgg gtgacgccga
     4081 tgacgtgagc ggtgacgtcg aggtcctggc cgctcgccgc cttcttgtgc acccgcacca
     4141 gccatcccgg ctccacggcc cgggcgaacg gggcgaggac cgtcgggttg ttctccaccc
     4201 ggaacgagat ctggcggacc tgcggggtcg cagtcccctg cgacgcgacg atccattcgg
     4261 cgatggcgag cgactgcttg tcggtcgggt gcagcagccc cgacagctgc accgcgttac
     4321 ggcgcccgaa cgcggcgacc gacgcggtgt tctcggcggt ctgctggcgg ccgtacgtcg
     4381 agttgaccgc ggcgacgttc gtgacggcga gcgggtcgtc gacgatgacg gtcccggtct
     4441 cagccatctc ctgggcgccg ccggccagca gggtcggatc atcggagaac gtcatctgca
     4501 cggtggtgga cacggtgtcg tcccacgccc acgtgcgttt cgagaatcgg aggtcgccgt
     4561 cgcggtcgac ccagatgcgg ccctgttcgg tgtcctcgac cgtctgcagg agaccggcga
     4621 cgtcaccggc ccgatacccc tggagggtgg tgatgccgga cgggtcgaac gtgccggtgt
     4681 ccgcggcgag ccccatggcg gtcacgacgt ggtccaggcg ttcgtccagc gagtcgcccg
     4741 cccacgcgac gcgacccgcc tcgtacagcg cggcgacgaa cgcgtcttcg gtcgggttcg
     4801 ctcccggagc ggcgaaggct cctgagccga agtcctcgaa caccatgacg tggtcgatca
     4861 caccctgata gggcaccgcc gaggggtcgt tgccctgccc gatgaacaag tcggtgagag
     4921 tcgctgcccg cgccccggac agagatgagc ccaacatgag gtcgacgggc accccgtcga
     4981 cccacagcaa cggttcctgc gctgcggtgt tgagcgcgac cgcgacatgg tgcacggtgc
     5041 cgtccgcgcc gagccacgcc cggtcgacgc cacccggtcc ggcgacggac ccccagaggt
     5101 tggccttcgg tgaggcggcg ccggtgtggc ggtcatcgac gaagacgaga gcgccggtcg
     5161 cggcgatctg cacgatgaga cacatcacgt cgcccggggc gcccggagcg ttgatgcact
     5221 gggtgagcag ccacagcggg gcggggctgc cgaggtagtg cagctgtcgg tcctccgggc
     5281 cgggcagctg cacccacatc gagatgatct gccgggacgt caggtcgatc ttcgagccgg
     5341 ggtcgacgac gacgccgtag ccgtcagcgt cggtctgccc ccatgtctgc gcatcaccgt
     5401 cgacgaccgg cccgaacggt gtctgcccgg cggagaacga ctcgaacccg ccggtgtgcc
     5461 gcgacgtgat cgacgtcatc ttgtcggtcc agccgccctc accgggccgc caccagtgcg
     5521 tcggagcgac cccggccgcg acctgggcgt cgaacgccgt gtcgggcgcg gcaccctcgg
     5581 cgagcacacc caggacgtcc tgtgcggtga cggtcacgac cggcagctgc gctgtcagct
     5641 gttgcggcca gccggagtcg acgaagcctg accagcgggt cgtggtcgtc gagttgtgcg
     5701 tgacccgcac ccgcaccggg gtcccgttgg tgaggtcgcc gtagtacggg ccgctcgggt
     5761 tccgtggatt gaacctgccc gtcgagttgt cgagcgtcaa cgacagcgac ccgggacgga
     5821 tggcccgcac cgtgtcgcgt cccgacgtcg cgacgatcgc cggtgacgac gagtcgaggt
     5881 ccacccagtc cgacaggtct acccagtcac cggcggcggg gcttgatccg ggaccgaacc
     5941 cgaacgccat ctcgatggtg accgcgaccc ggtcgaagct cacgccatgc tccgcgccag
     6001 ccgcgggaac ctgacgccct tagcgaccgc ggtttcgacc tgttcggcca cccacgagat
     6061 cgagtccgct gccaccggac ctgcgaacgt cacgtttacc gtgaccggcg acgacgctgc
     6121 cccgccggtt ggggcgaccc ccgatgatgc ggcccggacc gcgtcatcat gggtgatgac
     6181 cgtcccggaa gcgtcgggga caaacagctc ccagccgcgt tcgccgacga tgtacggcag
     6241 acccttcgtc accgggccac ccatagctcg ccgaccgggg gcgatgaggc cgccgagccc
     6301 ccacgtctgc atcatcatca ttccccgcaa cgcggcaagg cggttctgca cggtgccgat
     6361 cgcctgctcc gcctgggatg agtcgatgtg catcgaccgt ggcttgccga tctcctggcc
     6421 gagccaccac gccttggaac gggtttcgtc catcctgttt ccggccacgg cggtcgtcca
     6481 cgccaccccg tcgaactgcg acttcatcac cccggcctgc tgggccgtga tgacccccga
     6541 gctggccagg cggtcaaccg cacccttcgc ctggtcgatg ttcaccttgc cgttcgcgac
     6601 cgctgtcgac agggcaccca tccgaccctc gaggtcgaac tgcgcttgcg tcacagccat
     6661 cgtcgcggtc cgccacgcgt ccaggtcacc ggacgtgacg gcttcgtcga cccgcttctt
     6721 cgcgtccgcg acccggtctt gggcgtcgat gacggcgaac agcgggtcgg tgttcgctcg
     6781 cagctggtcg gcatagtcct gtagagccga ggtcgcgtct tcggtcgcct cggtgccgtc
     6841 ctcggtggac ccggtcatct cgtcggtcgc tcgtcgatcg gccttgaccg cggcaacgtg
     6901 ttgctccacc gccgcccggt tgcgctcgat gaactccatg ttcgtcttgt attgatccga
     6961 cgagtggtcg agcgactggg tctgtttctc cagggcgtcg agcacggcga gctgcttctc
     7021 ggggccatcg gtatcgccca aggtgtcgaa ggtccgctgc acctgctcga tgtccgcctt
     7081 gacgccggtc ccgacgatcg acacctcggc accgaactcc tgccacagat tctggagccg
     7141 cagcgtgtcc tgctcggccc cgaccatctc gccgaaggcg tcgacgacgt cgcccgcccc
     7201 gctgcccgca tcggcgaggg cgagcttcaa cttgtcgagc ccagcagctg ctcgagctgc
     7261 cgtcccactc gactggttca gcacggcgaa cacaccctcg accacgccga ccgccgcgcc
     7321 ggcagcggcg atccccgccg cggctttccc gatcccgttc agcgaacgga ccgcgttgtc
     7381 accctcaccg gagaccgacg tgaagcggtc gcgcatcttc aggagccagc cggcggcagt
     7441 gctgaggcca ccaacggcaa tgagcgacgc gccgccgatc gccccgagct ttccgacggt
     7501 gttagccagc tcggggttcg cggccgacgt cgcccccacc aggccggaca ccttgtcggt
     7561 gatcccgccg aacacctcga tggcgccacc gccgacgcct tcgacgatgt cgccgagctg
     7621 gttcttcatc cgttccatct gcccggagaa cgtcgcgccc tcctgttcgg cgaacccgcc
     7681 gaccgtcgag cgcaacgtgt ccaccgtggc attgaacgga tcgagcttga acgccgcctc
     7741 gtcgacgttg atccccatgc gcttgagtga cgtggagcta ccgtcgacgg ccttcagtgc
     7801 ggccttggcg gcggtgtcga ggtccacccc cattttctgg ctcaggtcga cgacgagcgg
     7861 cgtcagcgac gtcacctggg cctcggtgag cccgaactga accatcagtg cttgggcccc
     7921 gactatggcg tcggcgtcgg cggcggtctt cgactggatg tcggcggcga gatctttgag
     7981 cgccttgccg ttctccttga aggtctgcga gctgttcgcg atcgagttgt cgagcttgcg
     8041 gacctggcgt tctgcgttct cggactcctt cgccgcccag agcagtccgc cgacgagggc
     8101 gacccccgct ccggtggcga tgcccccgac tttcatcagc gactcgccca tcttgtccga
     8161 gtcggaacgc acgtcgagca tcgcccgagt ggcgtccttg gcattgccga ggatctcgat
     8221 ggtgagcttg cgtgtgttcg acaccccggt caccactcct cgacgtcgac tgtcctggtc
     8281 aggtctgcga tgaacctgtt gatctcatct ggggtcaggc gatcgacgtc ccacggatgc
     8341 agcccgtatc gggcggcgag cgccggccac agcggcaaca gcgcccgcct cacgcttccg
     8401 ggtcggactc gtcttcgggc gtctcgccgg tcaggacctc gccgtcggcg atgtcgccca
     8461 ggtcgacctg ctcgaacagg tcgctgatgc tcagctcggg ctgggatcgt gtcagtacga
     8521 cccaggccaa ggcggcgagc tggtcgacga agtcggggct caggtcgtcg gcttcggtga
     8581 gctcgacgag cgcccgtcgg gaccgccgcc gttcgccgag ggtgaaggtg cggtgatcga
     8641 ctgtgacctc ttcgccctga tagcggatcc tgaacgcggg tatcaccccg acttgttcga
     8701 tggtcacgct ctgctcctgt tctgttcagt ccgggaacgc cgggcgaacc aggcggtcga
     8761 tctcgtccat gtacgcggcc atgatccgct cacggttgtc gcggatcgtc gggaagatcg
     8821 cgtacccggc accgtcacca gcgccgcgcc actcttgaaa ctggttccag ccgagcatcg
     8881 agtgagatcc tcgccggcgc tgcacgttgc ggtgggcgcc gaactcgacg ccgagcgcga
     8941 acggcacgga cgcggaccct aagttgatgc gggcgcccac cgctgaacgc gacgccttga
     9001 tcgtcgacgc cgcacgacct gccatccctc cgacgggcag gcccgccatc acttggcggg
     9061 catcgaccgc gacgaggtcg gccacccgat ggtggacctt cttgaggtcc ttttcgaggt
     9121 cgatgtcctt gagcttctgg cgaagctctt tcagtccacg gacttcgact gtcgcggacc
     9181 ggttcaccgg catggcgtca gggggccgcg gaagcgacct ggtaggcgac gctgatcggc
     9241 gagctcgtgc cgtcgtagag gccgacgccc ttgagggact ggtcgacggc gtcgggtcca
     9301 ccgaccgcgg gggtgtcgcc gtcgaacatg acgaccggca ccgtgatggt caccttcggc
     9361 ttgagggtgg taccgatcgt ggtgagccct tcgcagacga ggacgacctg cgcctgggcg
     9421 cccgccgcgg tggtcgacag cacccggttc tggtgggtga ggttctcgaa gtcgcacttg
     9481 agctcgacgg acaccttgcg ggtcccggac tcgaccggct ccttcttcaa ggcgttcgac
     9541 cgcaggtagc ggcggtcggt cttgagcgtg ttgtcgacct tgacggagaa ctcggcgacg
     9601 tcgacctggg tgccgccgac ggtgacgacg ccacggatga acgtgatcgg ttcgaccgcg
     9661 gcggggtagc tggcggtcgc gagcccggtc gtgtggtcga gggtctggcc gtcgatgtcg
     9721 gccttgaact tgagggtctc accggctttg caggccagct cccacgacgt caccttgcag
     9781 ccggtgacgg tcttcggggt gatggcgccg ccgccgatct gggggacacc gacctgcagc
     9841 gtgaagaagt cgccggtgag gtcaccgacg gtgccggtgt gggtgtagac gctggtctct
     9901 gcggggccgg tggtggcgac ggtgccgagc atgtgcttga gccaccacga ccagccgagg
     9961 gacagcacgt caagctcgat cgagccggtg accccgttga tgtacgggac gcggcggtcc
    10021 gagcgcagga cgcgggtccc ggcccggatg ccgggcgatt cgacccggcc ggtctccgct
    10081 gcgaggccct cggagaggaa ctcgaagaac cggtcggggg tgacgagggt gccgtagatg
    10141 acctcgtcct tgacgccgag ttgtccgagg aatccgctca tggctgctca cccttcttgg
    10201 ttgccggctt ggccggcggt ttggactgct cccagatgtc ggtctgggtg acgagcccgg
    10261 cggcgacgtc gtcggggatg tcgacgtcga cgccgtgctc ggcctgccag atgcgtccgc
    10321 cggcggcgat ctccacgccg tcggtgaacg ggccgatgta gcgcaccttc acgggcgcac
    10381 ctccatggtt caggtccggg cccggaaccg gaccgcggac tggatgacgt gcccgtagcc
    10441 ctcgtcttcg acggtggggg ttgaggcggg gtgggcgatc tcgttccaca gcacgccggc
    10501 ggaatgcgcg agcgcgatgt tgtcggggtt gcggagctgg tcttcgatga cggcgaacaa
    10561 ggcgtaggca cggtcgcatg cttcgagcgc gtcatggccg ggccagcgga cgatgacggt
    10621 gactccgatc tcgaagtcgc agtcgcggcg gcgggcgccg agctgtgccc agtcttcggt
    10681 gccgtcgacg gggccgagga tgaccatctc tcggccgagt tcggcgggga caccgaagcc
    10741 gatgtcgggt tcccggtcgg gccacgggca gccgtcgacg agcaggtcgt gcagggcccg
    10801 caccgcggcg ggcacagcgg actgcgccat caggcgatcc ctggggtgcg catcgaccag
    10861 cggtcgagga cgacgtcgac gtcggggatg ccggtcttcg agccgcgttg gccggggacg
    10921 atgagggcga acgtgccgcc gttctcaccg gaggtgaagc tcgaggtgcg gtccgggact
    10981 ccgcggttcg atcggttcaa cacgtcgcgg acgcgggtga ggaaggcgcc gagcacgtcg
    11041 gcgggtgggc ggtcgaagcc gtgttcgtag ccgatgtgcc acggcgtcga tggccacgtc
    11101 ccggagggga gctcggcgac accgtcggct gatgcggcca ccgctgcgag ctgcggggcg
    11161 gtgagcccgt cggcgtatcg gacggtgcgc agcatcgggt cgtcgagcac gacccgggtc
    11221 gcgcccgacg cgcgcatcgg ggcgagccgg tagcggggca cgaacgcgac gccgcagatg
    11281 tcctcgaact cggtttcaac gaggcggcgg gcggcgacca ccgcggccga cgggtacttg
    11341 gcggtgtcgg tgagcgccgg gtccgacgcc cggatgtcgg ccgtcgacgc gtagtaggcg
    11401 gcgacgacct cgatgcgggt cgtcacctct tgggtgccgt caccccaggt ggcggtcagt
    11461 tcgtcgagcg tcgtggtctg cgtggcgttg agcgccgcct ggcggacacc gacggtgcct
    11521 gacggggtgg tggtcgccgc ggcggtgagg actgcggtcc cgtcggagcg ggtgacggtg
    11581 acggtgacgg ggccggtcgg gtcgaccagg tcgccgtcct ggtcgcggaa ccggccggtg
    11641 atcgtcgccg acgaacccgc gaggatctgt tcgtcggcga cgatcatcag tcggccttct
    11701 tgcggcctcg cccgcggccg gtggcgggct tgtcggcggt ctcgacggcg gggtcgtcgg
    11761 cgactgcggc ttcggtcacc ggtgcggcga cctcggcgct ggagaggcgg tcgatctcgg
    11821 cgcggacctg ggcggcccgg tccgttcgac cggatgcctc gtagccacgg agctcctcgc
    11881 gcaggcctcg caggtggtcg tcggtcgtca tggggttctc cttcatgttc gggccgggcc
    11941 cggtcgaccg cagcggggtg cggtcgaccg ggacgacctc aacgtcagaa cgtcggcgcc
    12001 gcgaggcccg tgccgctgat cgtggagatc gacgtcgggt accgacccgg cgtgaacaag
    12061 gagaagcggc cggcggcgag ccgcacctgg ccgggtgcgg tcgcgggcac ctgctcgaag
    12121 gtgaactgca gcaggtcgtc ctcccaatag aggatgtcga agctgcgggc cacgcagatg
    12181 acgtcctcgg tgccgccacc gagggtggtg gggatggagg cgtcggtgat gaccggcagg
    12241 ccctgaagcg tgccgacgac ctggccgtac tcggcggcct ggccgagccc gatcaccgac
    12301 tgcggcggcg tggtcgagaa ctcgaacagg ggccggccgg aggtgtcgac cgcggcggtg
    12361 atccagcccc aacgacgggg gtgcatgtag atcacgttgc cgggcatgaa ccgcgccgag
    12421 ttgatgcgct ggatcgcgtc cgccagcttc ggccacgcct cggcgaccgt cggcgaggcg
    12481 t
//
LOCUS       DAOQOD010000088        12473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1786_length_12473_cov_11.714527, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000088 DAOQOD010000000
VERSION     DAOQOD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_12473_cov_11.714527"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1894
                     /locus_tag="PLZ72_09720"
     CDS             <1..>1894
                     /locus_tag="PLZ72_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36615.1"
                     /translation="RRLAPAAMALAMLTSVVACAPPTSPGGPTPQEQFCTMWDKVQEA
                     PPTQDEAVLVKDDVVAMATDTTVTGTDCTAPNAKVALDGATLAEGEEVLEKQAPNETA
                     KVAAVTGEEISAGEPVLDNLTVSALSAEIGTWGIRLRGNVNITLSGVTSTIGFVGTLS
                     DLNNWSIQLSSSSFTIPGITVSPVTFTGTLSMSYGVASLELTANASSVKVGDVSVTGA
                     SLKVKASETTGVSASIAGTIKVGPSTASGTVDVAFDRAGAIVSAKADISAHLVGNMAG
                     GKKVDLQGQLKIDGNAQQTSLSFSASGLVGDLQVNQANGSLTLATNKATFTGVLDVQQ
                     GANVVRFNGTIVWDGITASTPYLQVEGAGEISGTLDDGSTASIKGTLQTVVIGGQMRS
                     VVTGAIQVGTLKANGTAIVETNGATTTLEIDGQLADAGFAATLDGYVQITDGRAEIVE
                     LNAAVDGQVHLGDVTLSAANLHIASRYGSTLDVSFDGRLDVGSRATLTGSIAAQFGPN
                     GTLLSLTGQMGGSLQLDTWGIVDFSGGVVANPEQVTLTGSGGVHLINFPLGITFNGSF
                     TSRLDQPTWQLNGSGRFQIGSITVASARLSLSQTAGMKATRAGFYLSIIGIPTYLEAD
                     FYLKSTG"
     assembly_gap    1895..1994
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1995..3235)
                     /locus_tag="PLZ72_09725"
     CDS             complement(<1995..3235)
                     /locus_tag="PLZ72_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNA36616.1"
                     /translation="MASVDEGAVGRRRFLQGSAALAGVAALGGTAVACAPQPGEVVRR
                     GNAAEAGFDHIVVVMMENRSFDHFLGWLPGADGRQAGLTFLDGDGVAHDTHRLTEFAA
                     CDMEDPGHSVTAGRIQYNDGRVDGFLRDAPDTFPIGYFEADQLLFWGEAAPAWTVCDN
                     YFAATLGPTFPNRFFAHCGRTDRVTNTLEISTLPTIWDRLSDAGLEGRYYFSDAPFSA
                     LLGLRHLFITRTVEQFKQDAAAGRLPNVSFVDPRFLIPTPFGTSADYHPVSDIRAGCA
                     FLHEIYEAVTRSPNWERTVLVVNFDEWGGFYDHVVPTEGPDADPNTALRGFRVPCLVA
                     SPLAKRGHVAHDLYDHTSVLKMIEWAWDLPPLAPRDAAANNLADVLDLGGTPDLDVHR
                     WSLPEFQIQDCSTTWLDSIWRR"
     gene            complement(3331..4578)
                     /locus_tag="PLZ72_09730"
     CDS             complement(3331..4578)
                     /locus_tag="PLZ72_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA36617.1"
                     /translation="MTSTSTSDRSSRSSAPLVVLFLRGGMDGLSLLRPTGDSELDRLR
                     GPLAVAADSTVDVGDGFGLHPSMARTAARFTDGGVAFVPACGLPGASRSHFDAQFAVE
                     HADDPSSRAPTEGPSGWLGRHLAATAGSAVAPFRGVSLGQPQVPAILAGSPDVVAARA
                     IPDLALGPEMPARRGRRGDRRGRPDAATTATAVSIETLRSLWLDDGGPLGASAAAAFD
                     VLEKVTDRPPRADPAAPPRSEAERYVTGNGAEGEPGADERGASADPGVAELVELFGSD
                     LGTEVAVLNLGGWDDHNDLGATDGAFAARAAALDATIDALITGIPDVTVLAVSEFGRR
                     VATNDSGGCDHGRGGVALVAGTRVRGGIHGDWPGLADLDDGDVRTANDVRGVFAEVVD
                     SVLGGDPERIVPGAPGDRLSLFR"
     gene            complement(4575..6101)
                     /locus_tag="PLZ72_09735"
     CDS             complement(4575..6101)
                     /locus_tag="PLZ72_09735"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNA36618.1"
                     /translation="MSKLVLSRRQVLVAAAACGLVGTASCSGGTDAGAGDHPGSGRPG
                     ASPTTAPSDLPDGVAVARRFAFAPTVALIELARGDATAFFDQQLDPSLPERPEVAALL
                     SATDPGDVPTEGGPAARRAALGALVSSTLAAGAWSTGQLRAVMTDFWADHLHVSSSLA
                     PEVLFTPAYRRLLSDGALGRFDDLLVSSARSAAMLISLDNASSRADGGRVPNENYGRE
                     LLELQTVGLIGSDGRSVSGRDADAPYSEDDVKAVAHVLSGWSLERGTRRFAFRPAWHD
                     LGPAAGADVLGWTPAGSGEQDGEDLLRHLAQHPATSRHITWKLARRFVADDVAPDDEI
                     VDELAGIYREGDTDIGPVLRRIGALVGTGDRSLPTGSGAKLRRPLDLVAAMLGSTIAV
                     PDPATWQAALRPIGRLLQTLGQVPMGWPAPDGFPEVAGAWSGPGAVIGRWNLASAAAG
                     GFDVLSADPAVAPLVGDELVTTVTGIAPSDRLSEALAPVTEPRSARAVLFASPEFQLR
                     "
     gene            6198..7577
                     /gene="zwf"
                     /locus_tag="PLZ72_09740"
     CDS             6198..7577
                     /gene="zwf"
                     /locus_tag="PLZ72_09740"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNA36619.1"
                     /translation="MSATDADALVLFGITGDLAYKKLFPALYALVEQGRLRGPVIGVA
                     SSEWTLAQLEDRVRGSLAESGLTVDAAVLADLLGRLDYISGDYRSEDTFDRLRSALGS
                     ARLPVCYLAIPPSMFETVTEGLARIGVTDTARVVLEKPFGRDLESAEELNRIVLARLP
                     EDRVFRIDHFVGKEPVLNLLVFRFANSLLEPIWNRRHIERVEITMAESFDVQGRGAFY
                     DTVGAVRDVVQNHLLHMLCLLAMEPPVSEDADALRDEYTKVLRATRVADPGRVVRGQY
                     DGYRAESGVAADSDTETFVALPLEIDNWRWAGVPFLIRAGKSLPVTATEAVVTLREPP
                     RPLFAGPVDDPEPNRFRFRLGPDSFLRIEMQAKVPGDEMSSRTVDLDVDERHAFSGAP
                     AYERLIGDVIEGDSRLFARQDTVEEAWRVVQPLLSDRPPVIAYDPGTWGPAAASDLVP
                     DDHDWMPCA"
     gene            complement(7638..8168)
                     /locus_tag="PLZ72_09745"
     CDS             complement(7638..8168)
                     /locus_tag="PLZ72_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36620.1"
                     /translation="MGLFNSMANYAHKSELRESKDEIVCGEIIRGLSHQGWRPFRIVA
                     HSDRQQMGFKVPGDPVLSAAYLVVDRRSGSGALTQAMGSKTSIGFLAEMRNKVSDPDD
                     LATMWKTDLANLFKLPVAGEVRINHQLNTVNAKTYHLVDLDQYVQGHSVSAEPLVGWI
                     VGQFDALREQLRPLKK"
     gene            complement(8171..9148)
                     /locus_tag="PLZ72_09750"
     CDS             complement(8171..9148)
                     /locus_tag="PLZ72_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36621.1"
                     /translation="MPEPQTPVHWLDTAGTQQGPEPWETVQARVAHAQIPPSTQVWWD
                     GAPGWMPFDQVEAAPAPAPAPAPTPTPTPTPDATVTLAGSAPAEPTTAEPTAATPTPA
                     GASAAPTGASAALMSGLSDQELDDEFIGLVGRSWELYKDTEFATSIDEATLGGVITAL
                     VDCGFVLIDLETAGAAPVVTTTTTTGTAEPVVTGAGHQLRFEEPETQSRVTVQLEHLT
                     PDVATAKIIGHRANAVIGYGQRVKEFNKVGQALRQEVQSSMIVSPEPGTVSFDADLSS
                     GYVYAQIDLLLELDRYVSEDLSVDHDLLRRHLASVVYTMKTFVQMRFGS"
     gene            complement(9253..>9745)
                     /locus_tag="PLZ72_09755"
     CDS             complement(9253..>9745)
                     /locus_tag="PLZ72_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36622.1"
                     /translation="SAEASIASATVRALMAGTVVLLDADTVAGWRRVAPSLGQLHHAI
                     ADLRSQHPDVTVAVVADASIKWALPADEQPAFEQDIVTRAVVCAPAGTRDGTYGWMLA
                     VVERARRDAERVLVVTDRALGGVPVVRLTHAEGHFRFDLDGATVRDVTPSNPHRRRGR
                     HRS"
     assembly_gap    9746..9845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9890..11311
                     /locus_tag="PLZ72_09760"
     CDS             9890..11311
                     /locus_tag="PLZ72_09760"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNA36623.1"
                     /translation="MLSNTSDAAAVPAVPAPPMRDHLDPTQHREWGRSRRKACPRTIQ
                     SLWEMTDARDPVEILQRQEEHRLPFLVPVRHARMLTSPFAFYRGSAAVMSADLATTPS
                     TGIQAQLCGDAHLSNFGLFGSPERALVFDCNDFDETHPGPWEWDVKRLAVSAVLMAAD
                     RGWDTDAQRRLARAVGFGYRVSMADFASKGRLAVWYSKVTDEQLLALAKDDNRAAIEK
                     QFRKARLNDVGRAIAKYTEVVDGRRRFVHQPPLVMRLDDSMSADTAAAMHQNVIGLFG
                     EYLESVPDYLRVLLGGYELIDVALKVVGVGSVGTRCYIALVRGRDDDDLMILQIKEAE
                     HSVLEPYVDPIAYENQGHRVVVGQHLMQAASDQFLGWANVGHHDFYIRQFRDMKGTIN
                     LEKISEPMTEAYLLLCAWTLARAHARSGSRIAIASYLGRGQRFDDAISSFAMAYVLQV
                     HRDFDLFLDAARGGRIEVAESGY"
     gene            complement(11438..>12473)
                     /locus_tag="PLZ72_09765"
     CDS             complement(11438..>12473)
                     /locus_tag="PLZ72_09765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA36624.1"
                     /translation="FDVVDGRYRRTVLGVAGAAAVAAVAADAIARSRELAAGEVRGSS
                     EEPVHHDGGSNRDSPDRDLSDSGPDVVDPPRRRTHDLWKRVAGSSAAAAIGAAGAMIA
                     GTWATRTTARRLYDRRVLPDLGTGVLAHAVAILGWDFIYYWNHRFMHTSRYMWALHVV
                     HHSSERYNLSTALRQPVADSLGTFVPTGALGLLGVRPEIVETARGVNLIYQFWIHSTV
                     IPKLGPFERWFNTASHHRVHHGSNRRYLDRNHGSILIIWDRLFGTFEPEDDDEPVVYG
                     LTKNVNSFNPVTVMTHEYVDIARDVAGSDTWGDRLSFVFRGPGWAYDRHRERGTVGHH
                     IAEEAAPLAG"
BASE COUNT         1816 a         4237 c         4409 g         1811 t
ORIGIN      
        1 cccgacgact cgccccggcc gccatggccc tggcgatgct cacctcggtc gtggcatgcg
       61 cgccgccgac ctcgcccggg ggacccacgc cccaggagca gttctgcacc atgtgggaca
      121 aggtgcagga ggcgccgccg acccaggacg aggccgtcct ggtcaaggac gacgtcgtgg
      181 cgatggccac cgacaccacc gtcaccggca ccgactgcac cgctcccaac gccaaggtcg
      241 ccctcgacgg cgccaccttg gccgagggtg aggaggtcct cgagaagcag gctccgaacg
      301 agacggccaa ggtcgccgcg gtcaccggcg aggagatctc cgccggcgag ccggtgctcg
      361 acaacctgac cgtcagcgcc ctgtcggccg agatcggaac gtggggcatc cggctgcggg
      421 gcaacgtcaa catcaccctg tcgggcgtca cgtcgaccat cggtttcgtc ggcaccctca
      481 gcgacctgaa caactggtcg atccagctct cctccagcag cttcaccatc cccggcatca
      541 cggtcagccc tgtcaccttc accggcacgc tgtccatgag ctacggcgtg gcgtcgctcg
      601 agctcaccgc caacgcgtcg agcgtcaagg tcggcgacgt ctcggtcacc ggcgcctcgt
      661 tgaaggtgaa ggcgtcggag accaccggcg tgtccgcctc gatcgccggc acgatcaagg
      721 tcggtcccag caccgcgagc ggcaccgtcg acgtcgcctt cgaccgggcc ggcgccatcg
      781 tgtcggccaa ggccgacatc tcggcccatc tggtcggcaa catggccggc ggcaagaagg
      841 tcgacctcca gggccagctc aagatcgacg gcaacgccca gcagacctcg ctgtcgttca
      901 gtgccagcgg tctggtcggt gacctccagg tcaaccaggc caacggctcg ttgacgctgg
      961 cgaccaacaa ggccaccttc accggcgtcc tcgacgtcca gcagggcgcc aacgtcgtcc
     1021 gcttcaacgg cacgatcgtc tgggacggaa tcaccgccag cacgccgtac ctgcaggtgg
     1081 agggggccgg cgagatctcc ggaaccctcg acgacggatc gaccgcctcg atcaagggaa
     1141 cgctccagac ggtcgtcatc ggcggccaga tgcgctcggt ggtgaccggg gcgatccagg
     1201 tcggcaccct caaggcgaac ggcacggcca tcgtcgagac caacggcgcg accaccaccc
     1261 tggagatcga cggtcagctc gccgatgccg gcttcgctgc caccctcgac ggctacgtgc
     1321 agatcaccga cggtcgggcc gagatcgtcg agctcaacgc agctgtcgac ggccaggtcc
     1381 acctcggcga cgtcacgctc agcgcggcga acctgcacat cgccagccgg tacggatcga
     1441 cgctggacgt gtccttcgac ggtcgactcg acgtcggtag ccgagccacg ctgaccggtt
     1501 cgatcgcggc ccagttcggg cccaacggca ccctgctgag cctgaccggc cagatgggcg
     1561 gatcgctgca gctcgacacc tggggcatcg tcgacttcag cggtggcgtc gtcgccaacc
     1621 ccgagcaggt cacgctgacc ggttccggcg gcgtccacct catcaacttc ccgctcggca
     1681 tcaccttcaa cgggtccttc acctcccgcc tggatcagcc gacctggcag ctcaacggat
     1741 cgggacggtt ccagatcggc tcgatcaccg tcgcctcggc acggctcagc ctgtcgcaga
     1801 ccgccgggat gaaggccacc cgggcgggct tctacctgtc gatcatcggc atcccgacct
     1861 acctcgaagc cgacttctac ctgaagtcga ccggnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnncgtcgc cagatcgagt ccagccaggt ggtcgagcag tcctggatct
     2041 ggaactcggg cagggaccag cgatgcacgt cgaggtcggg ggtcccgccc aggtccagca
     2101 cgtcggccag gttgttggcc gccgcgtcac gcggggccag cgggggcagg tcccaggccc
     2161 actcgatcat cttcaacacc gatgtgtggt cgtagaggtc gtgggcgacg tggccccgct
     2221 tggccagcgg tgacgcgacc agacacggga cacggaaccc gcggagggcc gtgttgggat
     2281 cggcgtcggg tccttcggtc gggaccacgt ggtcgtagaa gccgccccac tcgtcgaagt
     2341 tgacgaccag caccgtccgc tcccagttgg gggagcgggt gaccgcctcg tagatctcgt
     2401 ggaggaacgc gcaccctgcc cggatgtcgg acaccgggtg gtagtccgcc gaggtgccga
     2461 acggggtggg gatgaggaag cgtgggtcga cgaagctgac gttggggagc cgaccggcag
     2521 ccgcgtcctg cttgaactgc tcgacggtgc gggtgatgaa caggtggcgc aggccgagca
     2581 gcgccgagaa cggtgcgtcg gagaagtagt agcggccctc cagaccggcg tcggagagcc
     2641 ggtcccagat ggtgggcagg gtcgagatct ccaacgtgtt ggtgacccga tcggttcgcc
     2701 cgcagtgggc gaagaaccgg ttcgggaagg tcgggccgag ggtggcggcg aagtagttgt
     2761 cacacacggt ccacgccggc gcggcctcgc cccagaacag gagctggtcc gcctcgaagt
     2821 acccgatcgg gaacgtgtcg ggagcgtctc ggaggaagcc gtcgacgcgg ccgtcgttgt
     2881 actggatgcg cccggcggtg acgctgtggc cggggtcctc catgtcgcac gccgcgaact
     2941 cggtgagtcg gtgcgtgtcg tgggcgacgc cgtcgccgtc gaggaacgtg agtccggcct
     3001 gacggccgtc ggctccgggc aaccacccca ggaagtggtc gaaggagcgg ttctccatca
     3061 tgaccacgac gatgtggtcg aacccggcct cggccgcgtt gccacgacgg acgacctcgc
     3121 cgggttgggg cgcgcaggcc accgcggtcc cgccgagggc ggcgacgccc gccaacgccg
     3181 cggacccctg caggaaccgt cgcctaccca ccgccccttc gtcgaccgat gccatcgccc
     3241 ggaccccctc cagccgtgcc gcgtcccgcc gcggacctga ccccacggta ccaacctcag
     3301 gttgtcgggg ccggggtgag ggtcgggact tcagcggaag agggagagcc ggtctccggg
     3361 ggcgccgggg acgatccgct cggggtctcc accgaggacc gagtcgacga cctcggcgaa
     3421 cactcctcgg acgtcgttgg cggtgcggac gtcgccgtcg tccaggtcgg ccagcccggg
     3481 ccagtcgccg tggatcccgc ctctgacccg ggttcctgcg accagggcga cgccgccgcg
     3541 gccgtgatcg cagccgccgg agtcgttcgt ggcgacccgc cggccgaact cggagaccgc
     3601 caggaccgtg acgtcgggga tcccggtgat cagcgcgtcg atggtggcgt cgagcgcggc
     3661 ggctcgcgcc gcgaacgcac catcggtcgc ccccaggtcg ttgtggtcgt cccacccgcc
     3721 caggttcagc acagcgacct cggtgccgag atcggagccg aacaactcga ccagttcggc
     3781 cacccctgga tcggccgacg caccccgctc gtccgccccg ggttcaccct cggcgccgtt
     3841 gccggtcacg tagcgctctg cctccgatcg tggcggggct gcggggtcgg cccggggtgg
     3901 ccggtcggtc accttctcga gaacgtcgaa ggccgcggcg gccgacgccc cgagcggtcc
     3961 accgtcgtcg agccacagcg accgcagcgt ctcgatgctc actgctgtgg ccgtggtcgc
     4021 cgcatcgggg cgaccgcgcc gatcgccgcg tcggccgcga cgtgccggca tctccgggcc
     4081 caacgccagg tccgggatgg cgcgtgccgc caccacgtcg ggggagccgg ccaggatcgc
     4141 cggaacctgc ggctgaccca gcgacacgcc ccggaacgga gcgaccgccg atccggcggt
     4201 ggcggccagg tggcgaccga gccaacccga gggcccctcg gtcggtgccc gtgaggacgg
     4261 atcgtccgcg tgctcgaccg cgaactgggc gtcgaagtgg gagcggctcg cgccggggag
     4321 tccgcacgcc ggcacgaagg cgacgccgcc gtcggtgaac cgggccgcgg tgcgtgccat
     4381 cgatggatgc agtccgaacc cgtcgccgac gtcgacggtc gagtcggccg cgacggcgag
     4441 ggggcctcgc agccggtcga gctcggagtc gccggtgggg cgcagcaggg acagcccgtc
     4501 catgccgccg cgcaggaaca ggacgacgag aggagcgctg gaccgggaag accggtcaga
     4561 ggtcgaggtg gacgtcatcg cagttggaac tccggtgagg cgaacaggac ggcgcgggcc
     4621 gagcggggct cggtgaccgg ggcgagcgcc tcggacaacc tgtccgacgg ggcgatgccg
     4681 gtcaccgtcg tcacgagctc gtcgccgacg agcggggcga ccgccggatc ggcgctcagc
     4741 acgtcgaacc ctccggcggc ggcggaggcg aggttccaac ggccgatgac cgctccgggg
     4801 ccggaccacg ctccggcgac ctcggggaag ccgtcggggg caggccaacc catcgggacc
     4861 tggccgaggg tctggaggag ccgaccgatc ggccggagcg ccgcctgcca cgtggcggga
     4921 tcgggcacgg cgatggtgct gccgagcatc gcggcgacca ggtcgagcgg tcgacggagc
     4981 ttggcgcccg aaccggtcgg caacgatcgg tcgccggtgc cgaccagtgc gccgatccgg
     5041 cggagcaccg ggccgatgtc ggtgtcgccc tcgcggtaga tgccggccag ctcgtcgacg
     5101 atctcgtcgt cgggggccac gtcgtcggcg acgaagcgcc gcgcgagctt ccaggtgatg
     5161 tgccggctgg tcgccgggtg ctgcgccaga tggcgcagca ggtcctcgcc gtcctgctcg
     5221 cccgaacccg ccggggtcca acccaggacg tcggcgccgg cggccggtcc gaggtcgtgc
     5281 caggcgggtc ggaaggcgaa ccggcgggtg ccgcgctcca ggctccagcc gctcagcacg
     5341 tgggcgaccg ccttgacgtc gtcctcggag tacggagcgt ccgcgtcgcg acccgacacc
     5401 gaccggccgt cggatccgat cagaccgacg gtctgcagct cgagcagctc ccggccgtag
     5461 ttctcgttgg ggacgcgccc gccgtcggcc cggctcgacg cgttgtcgag cgagatcagc
     5521 atggctgcgc tgcgggccga cgagacgagc aggtcgtcga agcggccgag cgccccgtcg
     5581 ctgaggagtc ggcggtaggc aggggtgaac aggacctcgg gggccaggct cgagctgacg
     5641 tgcaggtggt cggcccagaa gtcggtcatc accgctcgga gctgcccggt cgaccacgct
     5701 ccagcagcca gcgtcgagga caccaacgcg cccaacgctg ctcgccgcgc cgcaggacca
     5761 ccttcggtgg gcacgtcccc cggatccgtg gccgagagga gggccgccac ctcggggcgc
     5821 tccgggaggg acgggtcgag ctgttggtcg aagaaggccg tggcgtcacc tcgcgccagc
     5881 tcgatcagtg cgacggtggg agcgaacgcg aaccttcggg ccaccgccac accgtcgggc
     5941 aggtccgacg gtgccgtcgt cggtgacgcg cccggccggc ccgagccggg gtgatccccc
     6001 gcgcctgcat cggtcccacc cgagcaggac gcggtgccga ccaggccgca cgccgcggct
     6061 gcgaccagca cctgtcgtcg gctcaggacc agcttgctca tgggtcgaat gtcgaccccg
     6121 gagatgatcg tccgagccgc gaggtgtgag gagtgtgtga cggtcgcgct cgacggggtg
     6181 gccggtaggg tccgcccatg agcgcgaccg acgccgatgc cctcgtcctg ttcgggatca
     6241 cgggcgatct cgcctacaag aagctcttcc ctgcgctcta cgcgctggtc gaacagggga
     6301 ggttgcgggg cccggtgatc ggcgtggcca gctccgagtg gaccctggcc caactcgagg
     6361 accgcgtccg cgggtcgctg gccgagtcgg gcctcaccgt cgacgccgcg gtgctggcgg
     6421 atctgctggg acgcctcgac tacatctcgg gtgactaccg ctccgaggac accttcgacc
     6481 gcctgcgctc agcgctcgga tcggcgcgac ttcctgtgtg ctatctggcg atcccgccgt
     6541 cgatgttcga gaccgtcacc gagggcctcg ctcgcatagg ggtcaccgac accgcccggg
     6601 tcgtgctgga gaagccgttc ggccgggacc tggagtcggc ggaggagctg aaccggatcg
     6661 tgctggcgag gctgcccgag gaccgggtgt tccgcataga ccacttcgtc ggcaaggagc
     6721 cggtgctcaa cctgctggtg ttccgcttcg ccaactcgct gctggagccg atctggaacc
     6781 gacgccacat cgagcgggtc gagatcacca tggccgagag cttcgacgtg cagggtcgcg
     6841 gggcgttcta cgacaccgtc ggcgcggtgc gcgacgtcgt gcagaaccac ctcctgcaca
     6901 tgctctgcct gctcgccatg gaaccgccgg tgtccgagga cgcggacgcg ctccgggacg
     6961 agtacaccaa ggtgctgcgc gccacccgtg tcgcggatcc gggtcgcgtc gtgaggggcc
     7021 agtacgacgg ctaccgggcc gagtcgggcg tggcggccga ctccgacacc gagaccttcg
     7081 tggcgctgcc actcgagatc gacaactggc ggtgggccgg ggtgccgttc ctgatccggg
     7141 ccggcaagtc gctcccggtg actgcgaccg aggcggtggt gaccctgcgc gagccgccgc
     7201 ggccgctgtt cgccggtccg gtcgacgacc ccgagcccaa ccggttccga ttccgtctgg
     7261 ggcccgactc gttcctgcgc atcgagatgc aggccaaggt ccccggcgac gagatgtcga
     7321 gccgcaccgt cgatctggac gtcgatgagc ggcacgcgtt ctcgggtgcc ccggcctacg
     7381 agcgcctcat cggtgacgtg atcgagggtg actcccggct gttcgcccgc caggacaccg
     7441 tggaggaggc gtggcgggtc gtccagccgc tgctgtcgga ccgtccgccc gtcatcgcct
     7501 acgacccagg gacgtggggc ccggccgcgg cgagcgacct ggtccccgac gaccacgact
     7561 ggatgccctg cgcctgacgg cccgggtggt ccccaggttg gcgaggccgg cgggcctcga
     7621 ggctccggtg gggcccgcta cttcttgagc gggcggagct gctcgcggag ggcgtcgaac
     7681 tggccgacga tccacccgac gagcggctcg gcgctcaccg agtggccctg gacgtactgg
     7741 tcgaggtcga ccaggtggta ggtcttggcg ttcacggtgt tgagctggtg gttgatgcgc
     7801 acctcaccgg cgacgggaag cttgaacagg ttggccaggt cggtcttcca catggtggcc
     7861 aggtcgtcgg ggtccgacac cttgttgcgc atctcggcca ggaagccgat cgaggtcttg
     7921 gaccccatcg cctgggtcag cgccccggac ccgctgcggc ggtcgaccac caggtaggcg
     7981 gccgacagca ccggatcgcc gggcaccttg aagcccatct gctggcggtc ggagtgggcg
     8041 acgatgcgga acggccgcca cccctggtgc gacaggccgc ggatgatctc tccgcagacg
     8101 atctcgtctt ttgactcgcg cagctcggac ttgtgcgcgt agttcgccat cgagttgaac
     8161 aggcccacgg tcagctcccg aatcgcatct ggacgaatgt cttcatggtg tagacgaccg
     8221 acgccaggtg gcgccggagg aggtcgtggt cgaccgacag gtcctcggac acgtagcggt
     8281 cgagctcgag cagcaggtcg atctgggcgt acacgtaccc ggatgacaga tccgcgtcga
     8341 agctcacggt tcccggctcc ggcgacacga tcatggagct ctggacctct tggcgcagcg
     8401 cctggccgac cttgttgaac tccttgaccc gttgcccgta gccgatgacg gcgttggccc
     8461 ggtgaccgat gatcttggcg gtggccacat cgggggtcag gtgctcgagc tgcacggtca
     8521 cccgggactg ggtctcgggc tcctcgaagc ggagctggtg cccggcgccg gtcacgaccg
     8581 gttcggccgt gccggtcgtt gtcgtggtcg tgacgaccgg tgcggcgccg gcggtctcga
     8641 ggtcgatcag gacgaacccg cagtcgacca gggcggtgat gacgccgccg agggtggctt
     8701 cgtcgatcga ggtggcgaac tcggtgtcct tgtacagctc ccagctgcgc ccgacgagcc
     8761 cgatgaactc gtcgtcgagc tcctggtcgc tgaggccgct catcagtgcg gccgaggctc
     8821 ccgtcggggc tgccgatgcg cccgccggtg tgggtgttgc cgcggtcggc tcggcggtcg
     8881 tcggttcggc gggtgcggat ccggccagcg tcaccgttgc gtcgggcgtc ggtgtgggag
     8941 tgggtgtggg cgccggcgcc ggcgccggcg cgggggccgc ctcgacctgg tcgaagggca
     9001 tccaaccggg agcgccgtcc caccacacct gggtgctcgg cgggatctgg gcgtgggcga
     9061 cccgggcctg caccgtctcc cacggctcgg gtccctgttg ggtcccggcc gtgtccagcc
     9121 agtgcaccgg ggtctgtggt tcgggcacgt gtccctcctg ctcgagttcg tgcccggcga
     9181 tgtgctcgtc gtcgggcgtc ggggtcgagc gtaccggggg gtgtgcccgt gccggtggct
     9241 caggggtggt ggctaggagc ggtgacggcc gcgacgtcgg tgcgggttcg acggggtgac
     9301 gtcgcgcacc gtcgctccgt cgaggtcgaa gcggaaatgc ccctcggcgt gggtgagccg
     9361 caccaccggc acgccgccaa gcgcccggtc ggtgaccacg agcacccgct ccgcgtcccg
     9421 ccgtgcccgc tcgaccaccg ccagcatcca gccgtaggtg ccgtcgcggg tcccggccgg
     9481 ggcgcacacc acggcccggg tcacgatgtc ctgctcgaac gccggctgct cgtcggccgg
     9541 cagcgcccac ttgatcgagg cgtcggccac gacggcgacg gtgacgtcgg gatgctggga
     9601 gcggagatcc gcgatggcgt ggtgcagctg accgagcgac ggtgcgaccc ggcgccatcc
     9661 ggccacggtg tcggcatcga gcagcaccac ggtcccggcc atcagggctc gaacggtagc
     9721 cgaggcgatg gacgcctcgg cgctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnngggag caccgtcggg aacgaccggc ggttccgtag gctgacctcg tgctctcgaa
     9901 cacctcggac gccgctgccg tccccgccgt ccccgcgccg cccatgcggg accacctcga
     9961 cccgacccag caccgggagt ggggacgctc gcgtcgcaag gcgtgccccc gcaccatcca
    10021 gtcgttgtgg gagatgaccg acgcacggga tccggtcgag atccttcagc gccaggagga
    10081 gcaccggctc cccttcctgg tcccggtccg ccacgcccgg atgctgacca gcccgttcgc
    10141 gttctaccgg ggatccgccg cggtgatgtc ggcagatctc gcgacgacgc cgagcaccgg
    10201 gatccaggcc cagctctgcg gtgacgcgca cctgtcgaac ttcggtctgt tcggctcgcc
    10261 cgagcgggcg ctggtgttcg attgcaacga cttcgacgag acccatccgg ggccctggga
    10321 gtgggacgtg aagcgcctgg cggtcagcgc ggtgctcatg gcggccgacc gagggtggga
    10381 caccgacgcc caacgccgtc tggcccgtgc cgtcggtttc ggataccggg tgtcgatggc
    10441 ggacttcgcc tcgaagggac ggctggccgt ctggtactcc aaggtgaccg acgagcagct
    10501 gctcgcgttg gccaaggacg acaaccgcgc cgcgatcgag aagcagttcc gcaaggcccg
    10561 cctcaacgac gtcggccggg cgatcgccaa gtacaccgag gtcgtcgacg gtcgacggcg
    10621 cttcgtgcac cagccgccgt tggtgatgcg actcgacgac tccatgtcag ccgacaccgc
    10681 cgcggccatg caccagaacg tgatcggcct gttcggcgag tacctggagt cggtgcccga
    10741 ctacctccgg gtgctgctgg gcggctacga gctgatcgac gtcgcgctca aggtcgtcgg
    10801 ggtcggcagc gtcggcaccc gctgctacat cgccctggtg cgcggccgag acgacgacga
    10861 cctaatgatc ctgcagatca aggaggccga gcactcggtt ctcgagccgt acgtcgaccc
    10921 gatcgcatac gagaaccagg gccaccgggt ggtcgtcggc cagcacctga tgcaggcggc
    10981 gtcggaccag ttcctcggat gggccaacgt cggtcaccac gacttctaca tccgtcagtt
    11041 ccgggacatg aagggcacga tcaacctcga gaagatctcg gagccgatga ccgaggcgta
    11101 cctgctgctg tgcgcgtgga ccctcgcccg tgcgcacgcc cgctcgggat cgcgcatcgc
    11161 gatcgccagc tacctgggcc gcggccagcg cttcgacgac gccatctcct cgttcgccat
    11221 ggcgtatgtg cttcaggtgc accgggactt cgacctgttc ctcgacgcgg cgcgcggcgg
    11281 acgcatcgag gtggccgagt cgggctactg acagcccacc gaacttgtag ctgggaggtc
    11341 gtgtgggcga ccgggagcta caacatcgac gaggccgagg gaacttgtag ctgggaggtc
    11401 gtggggacga ccgggagcta caagttcggg gcggtgctca ccccgcgagg ggagcggcct
    11461 cctcggcgat gtggtggccg accgttcccc gttcgcggtg gcggtcgtag gcccatcccg
    11521 gtccacggaa gacgaacgac agtcggtcac cccaggtgtc ggatccggcg acgtcccggg
    11581 cgatgtcgac gtactcgtgg gtcatgaccg tgaccgggtt gaacgagttg acgttcttgg
    11641 tcaacccgta cacgaccggt tcgtcgtcgt cctcgggttc gaacgtcccg aacagtcggt
    11701 cccagatgat caggatgctg ccgtgattgc ggtcgaggta cctgcggttg ctgccgtggt
    11761 gcacgcggtg gtgcgacgcg gtgttgaacc agcgctcgaa cggcccgagc ttgggaatga
    11821 ccgtcgagtg gatccagaac tggtagatca ggttgacgcc ccgggcggtc tcgacgatct
    11881 cgggtcgcac gcccaacagg cccaacgctc cggtggggac gaaggtaccg agcgagtcgg
    11941 cgacgggctg acgcagggcg gtcgacaggt tgtagcgctc gctcgagtgg tgcaccacgt
    12001 gcagcgccca catgtagcgg ctggtgtgca tgaaccggtg gttccagtag tagatgaagt
    12061 cccaccccag gatcgccacc gcatgggcca acacgccggt gcccaggtcg ggaaggacgc
    12121 gtcggtcgta gagcctgcgg gcggtggtgc gcgtcgccca ggtgccggcg atcatcgccc
    12181 cggccgctcc gatcgccgcc gcggcggagc tcccggccac ccgcttccac aggtcgtggg
    12241 tccgtcgacg cggtgggtcc acgacgtcgg gccccgagtc ggacaggtcc cggtccgggc
    12301 tgtcgcggtt cgaacccccg tcgtggtgga ccggttcttc ggaggacccg cggacctcac
    12361 cggctgccaa ctcgcggctg cgggcgatcg cgtcggcggc gaccgcagcg accgccgcag
    12421 ctcccgcaac ccccagcacc gtccggcggt accgcccgtc gacgacgtcg aac
//
LOCUS       DAOQOD010000089        12362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1805_length_12362_cov_10.133583, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000089 DAOQOD010000000
VERSION     DAOQOD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_12362_cov_10.133583"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="rfbC"
                     /locus_tag="PLZ72_09770"
     CDS             <1..395
                     /gene="rfbC"
                     /locus_tag="PLZ72_09770"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNA36625.1"
                     /translation="LHYHLHQADYWYVPFGHARVVLHDLRQGSPTDGATQMIDLGEVD
                     GGENRHQGVYIPPGVAHGFASLSDMAITYLVDGYYNPADELGVAWDDPEIGADWGLTD
                     PILSARDQSNPRRSDIEPMWQPHSSLRT"
     gene            474..1391
                     /locus_tag="PLZ72_09775"
     CDS             474..1391
                     /locus_tag="PLZ72_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA36626.1"
                     /translation="MIAAVVPAHNEAAHILDVIDGLPDVVDHIVIVDDKSTDDTAALA
                     ESSRDPRVRVIRHEQNTGVGGAIISGHKLALELGSDIDVIFAGDDQMDPTYLPALLDP
                     IVHEGYGFTKANRFYSSTSYQGMPTYRVFGNVVLSLANKAASGYWNLFDPQNGYTATS
                     AEVLRRLDLDSISQGYEFENDYLLNLNILNVRAKDIPIPARYGAEVSGMKMRKVIPAI
                     GGMLFAGFFRRVLRKYVVGSFSPIALFLFSGLLLWLFSALFGAWTIYETIGPGAATTG
                     TVLLAVVPFLMGFQLILTALTLDIQASPG"
     gene            1448..2398
                     /locus_tag="PLZ72_09780"
     CDS             1448..2398
                     /locus_tag="PLZ72_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNA36627.1"
                     /translation="MTASPRVAVVGAGNMGANHARVVAASPRAELAMVLDVDADRADA
                     VARPLGVEGSTDLAAAAGCDAVVVAATTEVHLDIALPLLEAGVSCLVEKPLADDLVAV
                     DRLIGASETSGAALMCGFVERFNPAVVGARALLDGSPVHLLGIRHSPHNPRTRSSVID
                     DLLIHDIDLALDLFGGSVTSVSGGHWVSPEGVEELADCTMVFDHGAVATLSSSRTSQR
                     KVRSLSLSSVDLLIEIDLLRHDLTAYRHRSGEVVDRDGPIYRAETVVEIPFVRHTGEP
                     LAVQFDHFVDLVTSDASRDAERASLRAPHAAVAQIKAGVR"
     gene            2404..3495
                     /locus_tag="PLZ72_09785"
     CDS             2404..3495
                     /locus_tag="PLZ72_09785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNA36628.1"
                     /translation="MTEIHVPEEAEALVLECLRRGALAQGPRVAQLEDEFAALCGVEH
                     AVAVGNGTLALELALAVADLEPGDEVVTSPFTFVATVNAALAAGATVRFADIDPSTFN
                     MDPDAAAAALTERTRALVPVHLYGRPAPMDELSTLALRHDLVLVEDSAQAHGAAIGDR
                     RTGSWGLGCFSFYATKNMTTGEGGMVTTSDARRADRMRVLRNQGMRARYQYEVVGHNW
                     RMTDVAASIGLAELHVLDERTEARRRNAAALSERLAGIEGLVVPDAGGPDVRHVFHQY
                     TVRVTDDARLGRDELSDALGEQGIGSGIYYPRAAYDYDCYRTHPGVVIEPMPRAERAA
                     AEVLSLPVHPFLSDGDLDRIGDAVRSLLS"
     gene            complement(3520..4881)
                     /locus_tag="PLZ72_09790"
     CDS             complement(3520..4881)
                     /locus_tag="PLZ72_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36629.1"
                     /translation="MKVAFLVPSLRLSGGLNVVVEHGRRLARDHGMDVTMVITHHPGE
                     APWPYDGLDQVRIVDLADVADESFDLAVATWWDTVTHLPAIDAKRGVYFVQSLEDRFF
                     PEDDPQSVLAGLTYRLGLPVITEARWIADTFAATDPELPCWYVRNGVNKEIFPETLEV
                     VPRTAGPLRVLVEGNLDDRIKGVPEALDALARMAEPAEVTVVSAKGGSLPGAAVVGPL
                     SQRALSEVMAHTDVVLKLSRVEGMSGPPLEGFHRGATCILTPVTGHDEYLQHGWNGLL
                     TGWDDPDGTARQLDLLARDRHLLHFLRTNAAATARAWPGWDQAATMMAGSLRAAAAAP
                     GPNLAMLAPLLREARYQVARDSRRIHRLKAKVQATENHLHKVEGVLSRTGDLAHERGL
                     ALAELQVHADAVTAHRDELESRLTASAEDIATLERSRALRRLAAAEQLWRRIRARLGR
                     LRR"
     gene            complement(4878..5723)
                     /locus_tag="PLZ72_09795"
     CDS             complement(4878..5723)
                     /locus_tag="PLZ72_09795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA36630.1"
                     /translation="MTQWEERISGSSDVLVLGEHQARYGWVRRVIDEATTWCDLGCGT
                     GAASSRALGDVLPENVVLIDVEPDALEEARRAFAPSPVRTAQVDLTSTAELAELASQL
                     EATPGPLVITCFEVIEHLVDLGPLVEWLGRRSQDGATVAISVPNDVFTSVKNPFHLTM
                     WGSSTVEELRGLLPDPHVVAVQAALSGSVVTDGTSVSFTGIVEDATSVVPLQYLMGFG
                     PSADRLAPNAVVLTTDIAAQRVWERQREVDNAYYRAMAARVPELEARIAELEARSDST
                     GAASR"
     gene            5766..6788
                     /locus_tag="PLZ72_09800"
     CDS             5766..6788
                     /locus_tag="PLZ72_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36631.1"
                     /translation="MSTPPTAAVTTPTDGELAWWAPLSVLRPDVGARLPPVGRPFRVA
                     FVGQRTYFEVCSQSSPSPVVDPTFVEFRSGSDGTALLARLRSLDPHVVVVFRPEIVPA
                     GLLRDLDALTLGFLTEPLPRPDEVGHPDLDRRLQDLAMVDADQFDRIVAFDPHIRTAA
                     ARYLPVWRAEPLPVADEVFSPRLEPRSGSRSLFVGRSTPHREEWLLPAKHLYDLMHVE
                     HGVFGPDLADLARDFLVAINLHNEPYPSFENRVPLHLAVGNLVISEALSPTNGLEPGL
                     DFLLAEDADDLVSYLGLIADDPQRFHPVRARGHLKAEAFRASRVYARLLLDLLLDVAE
                     FGRRSP"
     gene            6785..7552
                     /locus_tag="PLZ72_09805"
     CDS             6785..7552
                     /locus_tag="PLZ72_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HNA36632.1"
                     /translation="MSAPAADPGSGAPDPTLFLSSSTEALPEPIELHMDEPVVEFWRR
                     RIAQHLTEYYAGIRLSKFPEDLRVLEQLLFAQRPDAVIEIGCQFASSSLWIADRLASI
                     RRYGGPTDPLVVALDIDTAAATVALDTADPAWRDRIALIEADITDPSLPARVEDALGG
                     RRNCMVIEDSAHTYETTIAALKGFARFVAPGGYLIVEDGVVDVEELRLRPDWPRGVHR
                     AIAEFLSGDSGVEFVRRSDLEPYGLTSHPGGYLQRQG"
     gene            7619..8587
                     /gene="rfbB"
                     /locus_tag="PLZ72_09810"
     CDS             7619..8587
                     /gene="rfbB"
                     /locus_tag="PLZ72_09810"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNA36633.1"
                     /translation="MRLFITGGAGFIGSNYVRHVLTTSDDEVTVFDALTYAGNLENLA
                     DVAGDPRYRFVHGDICDRDAVAAAMDGHDAVVHFAAESHVDRSLLDPDVFVRTNCSGT
                     NVLCDTATRIGVERFVHISTDEVYGSIDEGSFTETDPLAPRSPYSAAKAGSDLIALAY
                     HETYQLPVLVTRSSNQFGPYQFPEKLIPYFVTSLLDGKKVPLYGDGLNVRDWLFVRDN
                     CTGVDLVLRNGTVGEIYNIGGGNERTNRDITDRLLSMLGKDDSSVDYVEDRLGHDRRY
                     SITSDKVGALGWSPSTPFEDALDETVAFYRDRRDWWEPLTARVKNR"
     gene            8596..9444
                     /gene="rfbD"
                     /locus_tag="PLZ72_09815"
     CDS             8596..9444
                     /gene="rfbD"
                     /locus_tag="PLZ72_09815"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNA36634.1"
                     /translation="MRIVVTGSNGQLGTEVMDLLGRSEHHEVLGLDLPDHDLTDRDHV
                     LGVVTSFEPDLILHGAAFTAVDVCEEQVETAYRVNCAATRFVADGARRVGAHVVYVST
                     DYVFDGTKTTPYVEWDGPNPQSVYGRTKLGGESEIDPGWTIARTSWVCGRHGNNMVKT
                     LLRLAGERDTLSFVDDQIGHPTFAQDLAAMVVKLGVERVPGVFHTTNQGAVSWYGFAR
                     AVFAAAGHDPDRISPISTAELDPPRPAPRPANSVLDNLAWRLHGFAPSRDFHEPLDEV
                     VASLRA"
     gene            9521..10327
                     /locus_tag="PLZ72_09820"
     CDS             9521..10327
                     /locus_tag="PLZ72_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA36635.1"
                     /translation="MQRLREIWAYRTLIANLAQRELRAKYKKSVLGWGWSLISPAATL
                     GVYTLVFGVYLKAVPPTMANGMANFALYLFAALIVWNGFSNVVNGSMGSLVDSGGLLS
                     KIYFPPECPLIAGVIASLLQVAIESAILVAVMIVIGNVSWTFVWLPVIVVETMAFALG
                     LGMIASLYNIIYRDVSYLVGIGMQILFYGTPIVYQLTDVPEKIGGIPVRAIVELSPLT
                     QYTIQSRQVTYLLEFPTLRQVGYTFVVSLITLVFGWWVFSRRAADVIEEI"
     gene            10327..11559
                     /locus_tag="PLZ72_09825"
     CDS             10327..11559
                     /locus_tag="PLZ72_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36636.1"
                     /translation="MSQIVVDDVSKRFRLYTSRANSLKERFTARRFARYEDFWALRDV
                     SLEIPPATTFGLIGHNGSGKSTLLRCIAGIYQPTSGTVSVDGRISALLELGAGFHPDL
                     TGKENIYLNAAILGLSTKEIDAVFDDIVDFSGIAGFIDAPVKVYSSGMFVRLGFAVAV
                     HVNPEILLIDEVVAVGDEEFQRRCFEHIHSLRRSGVTIVLVSHNLGLVQSMCDHAAWF
                     DHGRIQALGTSTEAASAYLSKVNAEEQEGAETPSAEGLTDDVLRRGSGEIRFTDVAVL
                     DRGRTRTMFAAPGEDITVRMTFDCHEPVVDPVFGVGIRHEGGQLMSLKTTLQSELDPG
                     TVREGGAVEFHIPRLPLLSGKYFVNVYAVDSHSTHVYDEIEHGLTLNVRGGLVRGIDG
                     FFDIEGDWTLRGAHEEAR"
     gene            11556..>12362
                     /locus_tag="PLZ72_09830"
     CDS             11556..>12362
                     /locus_tag="PLZ72_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36637.1"
                     /translation="MSRARAVVRRGAARALPPGSPGRGYASVALDTYREGRNALRRLS
                     DSVAVLHDDGRRPPDLATWQARTRVGPDELTRMEQTAATSANPTQVHVFVVADRGPTA
                     RSVRSLEQQVWPRTAHRVGPLSEVPAWTDTLPSDAMCVVLRAGDTIDRDVTFRVADEL
                     WSDPALHLVTWDDDVDGERDPRFAPPTWAPELLLSTNPAGRSFAVRVDRLRAAGGFDP
                     SSADPWWDLLLRLDPEPHRVAHLDGAPGHVVDRRRAAVEFDRAGGPFAHPA"
BASE COUNT         1814 a         4438 c         4149 g         1961 t
ORIGIN      
        1 gtctgcacta ccacctgcac caggccgact actggtacgt gccgttcggc cacgcccggg
       61 tggtgctgca cgacctccgt cagggcagcc cgaccgacgg cgccacccag atgatcgacc
      121 tgggcgaggt cgacggcggc gagaaccgcc accagggcgt ctacatcccc ccgggggtgg
      181 cgcacggctt cgccagcctg tctgacatgg ccatcaccta cctcgtcgac gggtactaca
      241 accccgccga cgagctgggc gtggcgtggg acgatccgga gatcggggcc gactgggggc
      301 tcacggaccc gatcctgtcg gcacgggacc agagcaaccc gcgccgttcg gacatcgagc
      361 cgatgtggca gccccacagc tcgctgcgga cgtgaccggg cagctgtcgt cgcgccgccg
      421 accccggcgg gtcggcgctg ccgaccggga ggcgtgacgt gtacgagggt cgggtgatcg
      481 cggccgtggt gccggcgcac aacgaggccg cccacatcct cgacgtcatc gacgggctgc
      541 ccgacgtcgt cgaccacatc gtgatcgtcg acgacaagag caccgacgac acggcggcgc
      601 tggccgagag cagccgtgac ccccgggtca gggtgatccg ccacgagcag aacaccgggg
      661 tcggcggtgc gatcatctcg ggccacaagc tggcgctgga gctgggctcg gacatcgacg
      721 tgatcttcgc cggcgacgac cagatggatc cgacgtacct tccggccctg ctcgacccga
      781 tcgtgcacga gggctacggc ttcaccaagg ccaaccgctt ctactcgtcg acctcctacc
      841 aggggatgcc gacgtaccgc gtgttcggca acgtggtcct gtcgctggcc aacaaggcgg
      901 cgtcggggta ctggaacctg ttcgaccccc agaacggcta caccgcgacc tcggccgagg
      961 tcctgcgccg gttggacctc gactcgatct cgcagggcta cgagttcgag aacgactatc
     1021 tgctgaacct caacatcctc aacgtccggg ccaaggacat cccgatcccg gctcgctacg
     1081 gagccgaggt gtcggggatg aagatgcgca aggtcatccc ggcgatcggc ggcatgctgt
     1141 tcgccggctt cttccggcgc gtgctgcgca agtacgtggt cgggtcgttc tcgcccatcg
     1201 ccctgttcct gttctcgggc ctgctcctct ggttgttctc ggcgctgttc ggagcctgga
     1261 cgatctacga gacgatcggg cccggtgcgg cgaccaccgg tacggtgctg ctggccgtgg
     1321 tgccgttcct gatggggttc caactgatcc tcaccgccct gaccctggac atccaggcct
     1381 caccgggctg aatcccggcc gcttcgctcg acccgtaccg cccgatcctc caggagcaca
     1441 cgtcaccatg accgcatcac ctcgggtcgc cgtggtcggc gccggcaaca tgggtgccaa
     1501 ccacgctcgc gtggtcgccg cgagcccgcg ggccgagctg gcgatggtcc tcgacgtcga
     1561 cgccgaccgc gccgatgccg tggcccgacc gctcggggtc gaggggtcga ccgatctcgc
     1621 cgcggccgcc gggtgcgacg cggtcgtggt cgccgccacc accgaggtcc acctcgacat
     1681 cgcgctaccc ctcctcgagg ccggcgtgag ctgcctggtc gagaagccgc tcgccgacga
     1741 cctggtggcc gtcgaccggc tgatcggggc gtcggagacc agcggcgcgg cgctcatgtg
     1801 cggattcgtc gagcggttca acccggcggt ggtcggcgcg cgggcgttgc tcgacgggtc
     1861 gccggtgcac ctgctcggga tccggcattc gccccacaac ccccgcaccc ggtcctcggt
     1921 gatcgacgac ctgctcatcc acgacatcga cctggcgctc gacctcttcg gcgggtcggt
     1981 gacctcggtg agcggcggcc actgggtgtc acccgagggc gtggaggagc tggcggactg
     2041 cacgatggtg ttcgaccacg gcgcggttgc cacgttgtcg agcagccgga ccagccaacg
     2101 caaggtccgt tcgctgtcgt tgagctcggt cgacttgctg atcgagatcg acctgctccg
     2161 ccacgacctc accgcctacc gccaccgctc cggcgaggtc gtcgaccgcg acgggccgat
     2221 ctaccgggcc gagacggtgg tcgagatccc gttcgtccgc cacaccggcg agccgctcgc
     2281 cgtccagttc gaccatttcg tcgacctggt caccagcgac gcctcgcggg acgccgaacg
     2341 ggcgtcgttg cgggccccgc acgcggcggt ggcccagatc aaggccggtg tccgatgatc
     2401 gcggtgaccg agatccacgt gccggaggag gccgaggccc tggtgctcga gtgcctgcgc
     2461 cgaggggccc tggcccaggg gccgcgggtc gcccagctcg aggacgagtt cgcggcgctg
     2521 tgcggggtcg agcacgccgt cgcggtcggc aacggcaccc tggcgctgga gctggcgctc
     2581 gccgtggccg acctggagcc gggcgacgag gtcgtcacct cgccgttcac cttcgtcgcc
     2641 accgtcaacg ccgccctggc cgccggggcg acggtccgct tcgccgacat cgacccctcc
     2701 acgttcaaca tggaccccga tgccgccgcc gcggcgctga cggaacgcac ccgggccctg
     2761 gtgccggtgc acctctacgg ccgacccgcg cccatggatg agctgtcgac gctggcgctc
     2821 cgtcacgacc tggtcctggt cgaggacagc gcccaggccc acggtgccgc gatcggtgac
     2881 cggcgcaccg gatcctgggg tctcgggtgc ttctcgttct acgccaccaa gaacatgacc
     2941 accggcgagg gcggcatggt caccacctcc gacgcccggc gggccgaccg catgcgggtg
     3001 ctgcgcaacc agggcatgcg ggcccgctac cagtacgagg tcgtcggcca caactggcgg
     3061 atgaccgacg tggccgcctc gatcggtctg gccgagctgc acgtgctcga cgagcgcacc
     3121 gaggcccgtc gtcgcaacgc cgcggcgttg tcggagcggc tggccggcat cgaggggctg
     3181 gtcgtccccg acgccggcgg tccggacgtg cgacacgtct tccaccagta cacggtccgc
     3241 gtcaccgacg acgcccgcct cggtcgtgac gagctgtccg acgcactcgg cgagcagggg
     3301 atcggctcgg gcatctacta cccccgcgcc gcctacgact acgactgcta ccggactcat
     3361 ccgggagtgg tgatcgagcc catgccccgg gcggagcggg ccgcggccga ggtgctgtcg
     3421 ctaccggtgc acccgttcct gagtgacggg gacctggacc gcatcggcga cgccgtccgt
     3481 tccctgttga gctgaagtcg ccgctacggc gtgggcgcct cagcggcgga ggcgaccgag
     3541 tcgggctcgg atccgccgcc acagctgctc cgcggcggcg aggcgacgga gggcgcggga
     3601 gcgctcgagt gtggcgatgt cttcggcgga ggccgtcagg cggctctcca gctcgtcgcg
     3661 gtgcgccgtc acggcatcgg cgtggacctg cagttccgcc agggccagcc cgcgctcatg
     3721 ggccaggtcc ccggtgcggg acagtactcc ttccaccttg tggaggtggt tctcggtggc
     3781 ctggaccttg gccttgagac gatggatgcg gcgcgagtcg cgagcaacct ggtagcgggc
     3841 ttcgcgcagc agcggcgcca gcatcgccag gttgggcccg ggtgccgccg ccgctgctcg
     3901 cagcgaaccg gccatcatcg ttgccgcctg gtcccagccg ggccaagctc gggcggtggc
     3961 ggccgcgttg gtgcgcagga agtgcagcag gtggcggtcc cgggccagga ggtccaactg
     4021 acgggcggtg ccgtccgggt cgtcccaacc ggtgagcagc ccgttccaac cgtgctgcag
     4081 gtactcatcg tggccggtga cgggcgtcag gatgcaggtg gcgccacggt ggaacccttc
     4141 gaggggcggt cccgacatgc cttcgacccg ggagagcttg aggaccacgt cggtgtgggc
     4201 catcacctcg gagagggcac gctgggacaa ggggccgacg acagcggctc cgggcaacga
     4261 cccgcccttg gccgacacca ccgtcacctc ggctggttcg gccatgcggg cgagggcatc
     4321 gagtgcctcg ggaacaccct tgatgcggtc gtcgaggttc ccttcgacca ggacccgcag
     4381 gggtccggcg gtgcggggaa cgacctcgag ggtctcgggg aagatctcct tgttcacccc
     4441 gttgcgcacg taccagcacg gcagctcagg atcggtggcc gcgaaggtgt cggcgatcca
     4501 gcgcgcctcg gtgatgacgg gcaggcccaa ccggtaggtc aggccggcca ggaccgactg
     4561 gggatcgtcc tcggggaaga agcggtcttc caggctctgc acgaagtaga cgccgcgctt
     4621 ggcgtcgatg gcgggcaggt gggtgaccgt gtcccaccac gtggccacgg ccaggtcgaa
     4681 ggactcgtcg gcgacgtcgg ccaggtccac gatcctgacc tgatcgagtc cgtcgtaggg
     4741 ccacggagcc tcgcccggat ggtgcgtgat caccatcgtg acgtccatgc cgtggtcgcg
     4801 ggccaggcgc ctcccgtgct ccacgacgac gttgagtcct cccgagagcc ggagcgacgg
     4861 gacgaggaac gcgaccttca ccggctcgcc gcccccgtgg aatccgaacg ggcctcgagc
     4921 tcggcgatgc gtgcctccag ctccggaacc ctggccgcca tcgcccggta gtaggcgttg
     4981 tcgacctcgc gctggcgttc ccacacccgc tgggcggcga tgtcggtggt gagcaccacg
     5041 gcgttggggg cgagccggtc ggcggacgga ccgaaaccca tcaggtactg caacggcacc
     5101 accgacgtcg cgtcctcgac gatgcctgtg aagctgaccg aggtcccgtc ggtgaccacc
     5161 gagcccgaca gggcggcctg gactgccacc acgtgcgggt cggggagcag gcctctgagc
     5221 tcctcgacgg tcgagctacc ccacatcgtc aggtggaacg ggttcttcac cgacgtgaac
     5281 acgtcgttgg ggacgctgat cgcgacggtc gcgccgtcct gtgatctccg gccgagccac
     5341 tccaccagtg gaccgaggtc gacgaggtgc tcgatcacct cgaagcaggt gatcaccagc
     5401 ggaccgggcg tcgcttcgag ctgcgaggcg agctcggcca gctccgccgt cgaggtgagg
     5461 tcgacctggg ccgtcctcac cggtgaaggg gcgaaggcgc gtcgtgcctc ctccagcgcg
     5521 tcgggctcga cgtcgatgag aacgacgttc tcgggaagca cgtcgcccaa cgctctcgag
     5581 gaggccgccc cggttccgca cccgaggtcg caccaggtcg tggcctcgtc gatcacacgg
     5641 cggacccacc cgtagcgggc ctggtgctcg ccgagcacga ggacgtccga cgagcccgag
     5701 atccgttctt cccactgggt catgcacgct ccgtcgctgg ggtgcgagaa ctatagttgc
     5761 ggcccttgtc gactccgccg accgccgccg tgacgacccc gaccgacgga gagttggcct
     5821 ggtgggcacc gttgtcggtg ctccgacccg acgtcggcgc ccgactccca cccgtgggcc
     5881 gtccgttccg ggtcgccttc gtgggtcaac gcacctactt cgaggtgtgc tcgcagtcgt
     5941 ccccctcgcc ggtcgtcgac ccgacgttcg tcgagttccg cagcggatcc gacgggaccg
     6001 cactgctggc tcgtctccgg tcgctggacc cgcacgtcgt cgtggtgttc cgacccgaga
     6061 tcgtgcccgc cggactgctg agagaccttg acgccctgac cctcggcttc ctgaccgaac
     6121 ccctgccccg ccccgatgag gtcggccatc ccgacctcga ccgtcgactc caggatctgg
     6181 ccatggtgga cgcggaccag ttcgaccgga tcgtggcctt cgacccgcac atcaggaccg
     6241 cggcagcgcg atacctgccg gtatggcgcg ccgagccgct cccggtcgcc gacgaggtgt
     6301 tctcgccccg cctcgaaccg cggtcgggct cccggtcgct gttcgtcggc cgcagcacgc
     6361 cccatcgcga ggagtggctg ctgccggcga aacacctcta cgacctgatg cacgtcgagc
     6421 acggggtatt cggcccggac ctggccgatc tggcccgcga tttcctcgtc gccatcaacc
     6481 tccacaacga gccctatccc agcttcgaga accgggtccc gctgcacctc gccgtgggga
     6541 acctcgtcat cagcgaagcg ctgtccccga cgaacggtct cgagcccggt ctggacttcc
     6601 tactggccga ggacgccgac gacctggtga gctacctcgg cctcatcgct gacgatccgc
     6661 agcgattcca tccggtgcgg gcacgaggcc acctcaaggc cgaggccttc cgcgcgagtc
     6721 gggtgtacgc ccgtctcctc ctcgacttgc ttctcgacgt ggccgagttc ggccggaggt
     6781 cgccgtgagc gctcccgccg ccgacccggg gtcgggcgct ccggatccga ccctgttcct
     6841 gtcgtcgtcg accgaggcgc ttcccgagcc gatcgaactg cacatggacg agccggtcgt
     6901 cgagttctgg cgtcgacgga tcgcccagca cctgaccgag tactacgccg gcatccgcct
     6961 ctccaagttc cccgaggacc tccgggtgct ggagcagctg ctgttcgccc agcgacccga
     7021 cgcggtgatc gagatcggtt gccagttcgc cagcagctcg ctctggatcg ccgatcgtct
     7081 ggcgtcgatc cgccgctacg ggggaccgac cgacccactg gtggtggcgc tggacatcga
     7141 caccgccgcc gcgaccgtgg cgttggacac ggccgatccg gcctggcggg accggatcgc
     7201 tctgatcgag gccgacatca cggatccgtc gctcccggcc cgggtcgagg atgccctcgg
     7261 cggtcgccgc aactgcatgg tgatcgagga cagcgcccac acctacgaga cgacgatcgc
     7321 ggctctgaag gggttcgcac gcttcgtcgc cccggggggc tacctgatcg tcgaggacgg
     7381 agtggtcgac gtcgaggagc tgcgactccg gcccgactgg ccgcgtggcg tccatcgtgc
     7441 catcgccgag ttcctgtcgg gcgactcggg cgtcgagttc gttcgtcgct ccgacctgga
     7501 gccctacggg ctgacgtccc accccggcgg atacctgcaa cggcagggct gaccactacg
     7561 ctcgaccgct gcacgcgccg caccgcgcct gcccgaccgt ccagcccagg agccccacgt
     7621 gcgtttgttc atcaccggcg gagccgggtt catcggttcg aactacgtcc ggcacgtcct
     7681 gaccacctcc gacgacgagg tcaccgtctt cgacgccctc acctacgccg gaaacctcga
     7741 gaacctcgct gacgtcgcgg gtgatccccg ctaccgcttc gtgcacggcg acatctgcga
     7801 ccgcgatgcg gtggcggcgg ccatggacgg ccacgacgcc gtcgtgcact tcgcggccga
     7861 gagccacgtg gaccggtccc tgctcgaccc cgacgtgttc gtccgcacca actgctcggg
     7921 aaccaacgtg ctctgcgaca ccgccacccg tatcggcgtc gagcggttcg tacacatctc
     7981 cacggacgag gtctacggct cgatcgacga gggctcgttc accgagaccg acccgctggc
     8041 accccgctcg ccgtactccg ccgccaaggc gggttcggac ctgatcgcgc tggcgtacca
     8101 cgagacctac cagctgccgg ttctggtcac ccgttcgtcg aaccagttcg ggccctacca
     8161 gttccccgag aagctcatcc cgtacttcgt gaccagcctg ctcgacggca agaaggtgcc
     8221 gctgtacggc gacggcctca acgtgcgcga ctggttgttc gtccgcgaca actgcacggg
     8281 tgtcgacctg gtgctccgca acgggacggt gggtgagatc tacaacatcg gcggcggcaa
     8341 cgagcgcacc aaccgcgaca tcaccgatcg actcctgtcg atgttgggca aggacgactc
     8401 gagcgtcgac tacgtcgagg accgcctcgg ccacgaccgt cgctattcga tcaccagcga
     8461 caaggtcggt gccctcggct ggagtccgtc cacgccgttc gaggacgcgt tggacgagac
     8521 cgtggcgttc taccgggacc gcagggactg gtgggagccg ctcaccgccc gggtcaagaa
     8581 ccgctgaggt cgaccgtgcg catcgtcgtc accggctcca acggccagct cggcaccgag
     8641 gtcatggacc tgctcggtcg ctcggagcac cacgaggtcc tgggcctgga ccttcccgat
     8701 cacgacctca ccgaccgcga ccacgtgctc ggcgtcgtca cctcgttcga accggatctg
     8761 atcctgcacg gcgccgcctt caccgcggtc gacgtgtgcg aggagcaggt cgagaccgcc
     8821 taccgggtca actgcgccgc cacccgcttc gtggccgacg gcgcccgtcg ggtcggggcg
     8881 cacgtggtgt acgtgtccac cgactacgtg ttcgacggca ccaagaccac gccgtacgtg
     8941 gagtgggacg gtccgaaccc gcagtcggtg tacgggcgga ccaaactcgg cggcgagtcc
     9001 gagatcgacc ccggctggac gatcgcccgc acgtcgtggg tgtgcgggcg gcacggcaac
     9061 aacatggtca agacgctgct gcggttggcg ggcgagcgcg acacgctgag cttcgtcgac
     9121 gaccagatcg gtcatccgac cttcgcccag gacctggccg ccatggtggt gaagctcggg
     9181 gtggagcggg tgccgggggt gttccacacc accaaccagg gggcggtcag ctggtacggg
     9241 ttcgcccggg ccgtgttcgc ggcggccggc cacgacccgg accggatcag cccgatcagc
     9301 accgccgagc tcgacccgcc ccgcccggct ccccgtccgg ccaactcggt gctcgacaac
     9361 ctggcgtggc ggctccacgg gttcgccccc agccgtgact tccacgagcc cctcgacgag
     9421 gtcgtcgcct cgctccgggc ctgatcgggt cctgcggacc gagacaggcc ccctgctagg
     9481 gtctccgacc ggcggcaacg tcgctgtccc cccttcgcgc atgcaacgac tccgagaaat
     9541 ctgggcctac cggaccctca tcgccaacct cgcccagcgg gaactgcgag ccaagtacaa
     9601 gaagagcgtt ctgggctggg gctggtcgct catcagccca gcggccactc tcggcgtcta
     9661 cacgctggtg ttcggcgtct acctcaaggc cgtgccgccc accatggcca acggcatggc
     9721 gaacttcgcc ctctacctgt tcgccgcgct gatcgtgtgg aacggcttct cgaacgtggt
     9781 caacggttcg atggggtcgt tggtcgactc gggcgggctg ctgtcgaaga tctacttccc
     9841 gccggagtgc ccgctgatcg cgggggtcat cgccagcctg ctccaggtcg ccatcgagtc
     9901 cgccatcctc gtcgccgtca tgatcgtgat cggcaacgtc agctggacct tcgtctggct
     9961 ccccgtgatc gtggtcgaga ccatggcctt cgccctcggg ctagggatga tcgccagcct
    10021 ctacaacatc atctaccgag acgtcagcta cctggtcggc atcggcatgc agatcctgtt
    10081 ctacggcacg ccgatcgtgt accagctcac cgacgtcccc gagaagatcg gcggcatccc
    10141 ggtgcgggcg atcgtcgagc tcagccccct gacccagtac acgatccagt cccgccaggt
    10201 cacctacctc ctcgagttcc cgacgcttcg ccaggtcggc tacacgttcg tggtgtcgct
    10261 gatcacgctc gtgttcggct ggtgggtgtt cagccgccgc gccgcggacg tgatcgagga
    10321 gatctgatga gccagatcgt cgtcgacgac gtctccaagc ggttccggct ctacacgtca
    10381 cgggccaact cgctcaagga gcggttcacg gcccggcgct tcgctcgcta cgaggacttc
    10441 tgggcgttgc gcgacgtcag cctggagatc ccccccgcca ccaccttcgg tctgatcggc
    10501 cacaacggct cgggcaagtc cacgctgctg cgctgcatcg ccgggatcta ccagccgacg
    10561 tcggggaccg tctccgtcga cggtcggatc tcggccctgc tggagctcgg tgccgggttc
    10621 caccccgacc tgaccggcaa ggagaacatc tacctcaacg ccgccatcct cggactgtcg
    10681 accaaggaga tcgacgccgt cttcgacgac atcgtcgact tcagcggcat cgccggcttc
    10741 atcgatgcgc cggtcaaggt gtactcgtcg ggaatgttcg tgcgcctcgg cttcgccgtc
    10801 gcggtccacg tcaaccccga gatcctcctc atcgacgagg tcgtcgccgt cggcgacgag
    10861 gagttccaac gtcggtgctt cgagcacatc cactcgttgc gccgctccgg cgtgacgatc
    10921 gtgctggtca gccacaacct gggtctggtg cagtcgatgt gcgatcacgc ggcatggttc
    10981 gaccacgggc gcatccaggc cctgggcacc tccaccgagg cggcgagcgc ctacctgagc
    11041 aaggtcaacg ccgaggagca ggagggcgcg gagacgccga gcgccgaggg tctgaccgac
    11101 gacgtcctgc gccgcggatc gggcgagatc cggttcaccg acgtggccgt cctggaccgt
    11161 ggccggaccc gcacgatgtt cgcggccccc ggcgaggaca tcaccgtgag gatgaccttc
    11221 gactgccacg agcccgtcgt cgacccggtc ttcggcgtcg gcatccgcca cgagggcggt
    11281 cagctcatgt ccctcaagac gacgttgcag agcgagctcg atccaggaac ggtccgcgag
    11341 ggtggggcgg tcgagttcca catcccacgg ttgccgctct tgagcgggaa gtacttcgtg
    11401 aacgtgtacg ccgtcgacag ccactccacc cacgtctacg acgagatcga gcacgggttg
    11461 accctcaacg tccgtggcgg cctggtgcgg ggcatcgacg ggttcttcga catcgaaggt
    11521 gactggaccc tgcgaggagc ccacgaggag gcccgatgag tcgggcccgg gcggtggttc
    11581 gccgcggagc ggcccgtgcc ctgccgccgg gcagccccgg tcgcggctac gcgtcggtcg
    11641 ccctcgacac ctaccgcgag ggtcgcaacg ccctgcgacg cctgtcggac tcggtcgccg
    11701 tgctgcacga cgacggccgg cgaccgcccg acctggccac ctggcaggct cggacgcggg
    11761 tcggaccgga tgagctgacc cgcatggagc agacggccgc caccagcgcc aaccccaccc
    11821 aggtccacgt gttcgtcgtg gcagaccgag gacccaccgc ccgctcggtg cggtcgttgg
    11881 agcagcaggt gtggcctcgc accgctcacc gggtgggtcc gctgtccgaa gtccccgcct
    11941 ggaccgacac gcttccctcc gacgcgatgt gcgtggtgtt gcgggccggc gacacgatcg
    12001 accgagacgt caccttccgg gtcgcggacg agctgtggtc ggatccggcc ctgcacctgg
    12061 tcacctggga cgacgacgtc gacggtgagc gtgatccccg gttcgctccg cccacgtggg
    12121 ctcccgagct gctgttgtcc accaacccgg ccgggcggtc gttcgcggtg cgggtcgacc
    12181 ggctgcgcgc cgccggtggt ttcgatccgt ccagcgctga tccgtggtgg gacctcctcc
    12241 tgcgtctcga ccccgagccc caccgggtgg cgcacctcga cggggcaccc gggcacgtgg
    12301 tcgaccggcg ccgcgcggcg gtcgagttcg atcgtgctgg cgggcccttc gctcatcccg
    12361 cg
//
LOCUS       DAOQOD010000090        12327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1813_length_12327_cov_13.494296, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000090 DAOQOD010000000
VERSION     DAOQOD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1813_length_12327_cov_13.494296"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..888)
                     /gene="phoU"
                     /locus_tag="PLZ72_09835"
     CDS             complement(112..888)
                     /gene="phoU"
                     /locus_tag="PLZ72_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNA36638.1"
                     /translation="MDEMRIEFHEEMEALRSDVIRLGAMVCETVGRGTAALLDRDLHA
                     AQLLIDGDDVIDDFCLGLEERCCQLLALQAPIAGDLRFILTTLRLLSELERSGDLMVN
                     VCKASRRIYDIEFGPQLRGLIEHMGEEAAFLVRTAIDSYVDADTSLAAALDDIDDRLD
                     ELQTAYVQAIFTSHSVDNLNLQAAVQLAMIGRYYERIGDHAVNIGERVQYMVTGWLPD
                     HPGATRPAAPITPSPGSDGDALGPGTDSGPDSGPAPGEAS"
     gene            complement(928..1716)
                     /gene="pstB"
                     /locus_tag="PLZ72_09840"
     CDS             complement(928..1716)
                     /gene="pstB"
                     /locus_tag="PLZ72_09840"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNA36639.1"
                     /translation="MAPVVFDVSDLSVFYGDYRAVRDVSMRIRRNEITGFIGPSGCGK
                     TTVLRCFNRMNDLIEGARVQGRLDYHGVDLYAPDVNAVEVRRRIGMVFQKPNPFPKSI
                     YDNVAYGPRIAGTKKRSELDDIVERSLQRAALWDEVKDRLKSSALGMSGGQQQRLCIA
                     RAIAVDPEVILMDEPCSALDPIATARIEDLMKEIKTDYTIVIVTHNMQQAARVSDATA
                     FFSTEVNPDSDVRTGLLVEFDRTATIFSNPHDQRTENYITGRFG"
     gene            complement(1940..2971)
                     /gene="pstA"
                     /locus_tag="PLZ72_09845"
     CDS             complement(1940..2971)
                     /gene="pstA"
                     /locus_tag="PLZ72_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNA36640.1"
                     /translation="MNTTSTVPEDRTPDRVRRELSDANISIGRRVRNRAATVAMAVAF
                     LLAAGPLVLMAANVFSQGLPAVTNLDWWTQPIPADVGRADLAGNEQLQDLGFGDQAAD
                     PDSTASTDSADSSTDGLVALGMQPAIVGTFLTVLGASALAIPLGIMGAVYLNEYGKRN
                     RLANFIRFMTDVMTGVPSVVMGVFIYSIWVLRFGVEGKSAFAASLALACLMLPIVVRS
                     TEEMLRLVPDPLREASAALGTRTWKTTVGVTLPAALPGIVSGCLLAVARAAGETAPIV
                     FTIGFVTTTNWRFTGQNTTLSAQIYSQLQNGGDLATQMAWGAAVTLIGIVLVLTLIAR
                     FVARRYALR"
     gene            complement(2968..3969)
                     /gene="pstC"
                     /locus_tag="PLZ72_09850"
     CDS             complement(2968..3969)
                     /gene="pstC"
                     /locus_tag="PLZ72_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNA36641.1"
                     /translation="MTLELDTLPAAPDPEADRSAPDLTADGVGGRIDRSFRRVALAAG
                     LTVLLALLLIAVVTTNKAWPAFSELGGSYFFGTEWVPSEGLYGIVPLVFGTILVSVLA
                     LVVAVPISVGIALFVTEVVHRRARGAVTTTIDLLAAVPSVVFGLWGFYYLIPPFQTAF
                     NAVSDAVSGIPVLSTVLGPSTGSSYASAALVLALMVTPIITSVTREVFTTVPPNDKAG
                     ALALGATRWEMIRGVVFPHSTGGMTGAVMLGLGRAMGETVAVALLIGARPDITANIFA
                     VGETMPAQIFRNLSEADDLFRAALIGLGVVLFVLTILVNVSARRMVVVIDRRVKGAA"
     gene            complement(3989..5104)
                     /gene="pstS"
                     /locus_tag="PLZ72_09855"
     CDS             complement(3989..5104)
                     /gene="pstS"
                     /locus_tag="PLZ72_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNA36642.1"
                     /translation="MLHESRIVRLLAVIVVAVGLVATACGGSEESGSSADDGSSTTSG
                     AAGPAAPSNADIEALTAKVAGGGASFPDAFYQAVDADFNGVAGSELVTYAKSGSSDGR
                     KQLSSGTLDVAGSDSLPKPEETFPSQVLFFPTVAAPITISYNLAGVDDLRLSADTVAG
                     IFGGRITSWADPAIEADNPDATLPATKITVVHRSDGSGTTNNFTKYLSAAAPDTWTLG
                     SGDTVNWPAATQGAEKNSGVAALIGQTDGSVGYVDLADAIKAELTFASIKNAAGSFVA
                     PSAAATEAAVANADVAEDLTYNPLNAPGADSYPITAPTYLLVTRTQPDAARAATLKTY
                     LRYLLTTGQAQARKLGFVGLPDDLRQKAYDQVEEIGG"
     gene            complement(5267..5701)
                     /locus_tag="PLZ72_09860"
     CDS             complement(5267..5701)
                     /locus_tag="PLZ72_09860"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA36643.1"
                     /translation="MSRSGPAPDAPVVRAAGGVVWRRRRGAVEVVVVHRPAPRDDWSL
                     PKGKLEDGERHRDAALREVWEETGLRCSLGDRLVEIRYPTPRGEDKRVRWWAMTVDSD
                     DGFRPGHETDERRWVPLDDVDGLLSWDTDRTVLERFRSGSDV"
     gene            complement(5698..7767)
                     /locus_tag="PLZ72_09865"
     CDS             complement(5698..7767)
                     /locus_tag="PLZ72_09865"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HNA36644.1"
                     /translation="MTDPDERSPYLNRELSWLEFNARVLALAEDPATPLLERLKFLAI
                     FSQNLDEFFQVRVAGLKDRVAAGVTRRSPDGMSASEQLEAINRRSAELVAHADEIFLG
                     PICRNLAEEGIVFSTWNELDDDDREWATVEFRNRIFPVLTPLAVDPGHPFPYISSLSL
                     NLGVIVRDPTTDQRRFARVKVPSLLPRFVVMPDGERFLPLEQVIAHHLDELFPGMDVV
                     DHVAFRVTRNADLTVEEEEADDLLAAIEMELRRRRFGKAVRLQVEDDISAEALELIRD
                     ELELTDDDVSAHAAPLDLGGLFAVADADRPDLKYPEFVPVTQHRLALNEDEEPPDLFA
                     VMREGDVMVHHPYDSFSTSVEEFIRQAARDPKVLAIKLTLYRTSGDSPIVAALIKAAE
                     MGKQVVALVELKARFDEERNIEWARRLEQAGVHVVYGLVGLKTHTKTCLVVRDEGTSV
                     NRYCHIGTGNYNSKTARLYEDVGLLTADPDLGADLTQLFNYLTGYARDVHYAKLLVAP
                     HTLRNGLVRFIRNERAAAPGTGHIIMKMNSLVDADLIDELYAASQDGVRVELIVRGIS
                     CIRPGVAGLSDNITVRSIVGRYLEHSRIYRFANGRGPGQELHFIGSADLMPRNLDRRV
                     EAVVPVRSPELRVRMDEVLAVALADDTLAWEQVDDRWTHVPRVNTVETHLEQQEFAEK
                     RASVGRR"
     gene            complement(7816..8091)
                     /locus_tag="PLZ72_09870"
     CDS             complement(7816..8091)
                     /locus_tag="PLZ72_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36645.1"
                     /translation="MSDTYASESDEPIEWVAAVTIVYLGPVAPHWEIRGDHGDTQLID
                     DFRARVNARLMLLPPHDPQFKRNRERVERDAERELIELFWDLGDDER"
     gene            8187..8444
                     /locus_tag="PLZ72_09875"
     CDS             8187..8444
                     /locus_tag="PLZ72_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA36646.1"
                     /translation="MDQELDTEWMAHGNCADQPPSLFFPSDGVGVEVAKRICADCPMR
                     VRCLDYALDNRIDHGVWGGTSERERRRILKARQVRQPAGVG"
     gene            complement(8553..8747)
                     /locus_tag="PLZ72_09880"
     CDS             complement(8553..8747)
                     /locus_tag="PLZ72_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNA36647.1"
                     /translation="MFSGNEWLIVVIIALVIFGGSQLPKLARNLGSAQKEFKKGLAEA
                     SADDDDDAPTDPKASDKKSA"
     gene            8772..9461
                     /locus_tag="PLZ72_09885"
     CDS             8772..9461
                     /locus_tag="PLZ72_09885"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNA36648.1"
                     /translation="MTSSSEPSAAVPSPNDATRPVDPVEPVDPVDRARSHGRTARAAL
                     DPREREAATRSVVTRLSELAELGGREPGSVPGRVAISVAVRGELDVTGVAAVVRDRGV
                     EVWLPAVVGDSLVFREWRDGDRLVPGRFGIPEPPDDGRDTIDAAGLAVVVVPCVAVDE
                     HGTRVGSGFGYYDRALADGSDAGPTRPRRPVVVAAAFEVQVVGELPMRAWDVPADVVV
                     TERRTILTHRR"
     gene            complement(9468..9782)
                     /locus_tag="PLZ72_09890"
     CDS             complement(9468..9782)
                     /locus_tag="PLZ72_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA36649.1"
                     /translation="MSDATDAQPNQSAAEGVDEVAAESWQAFANCLGVDPDLFFPERG
                     ASTKEAKQVCQGCVVREDCLEYALANGEKFGIWGGLSERERRRIRRQRALARAAQQSR
                     TA"
     gene            complement(10160..12163)
                     /locus_tag="PLZ72_09895"
     CDS             complement(10160..12163)
                     /locus_tag="PLZ72_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36650.1"
                     /translation="MARPRFRYSPWDGTQTGFELDALDVMEQLTDDLLYHGDLNSALR
                     RMMQQGFEDRNGERMQGLREMLEQLREQRQDLLDRFDLGGVYDDIAGELNEVVAQERE
                     QLERDVQAARDSGDARRTELTEESAAGRNLELDMLPPDLAGKVRGLDDYDFASAEAAQ
                     RFEALKDRLREQLVQQAVDQMSGAMQNMSPQDMSRLKDMLAELNQMLETRARGEEPDF
                     DGFMERFGDFFPEGPENLDELLAIMAARMQAMAALMNSMTPEQRAQLQELSDQLLEDM
                     DLRWQMDQLGQNLRDAFPDLDWDASYRFRGDDPLDMAQASSVLGQLGDLDQLEQMMRG
                     GTDPGALRDIDLDRVRQLLGDNAADSLERMSEITKMLTDAGLVEQREGRLELTPKGMR
                     RLGQNALAQLFRKLDHDLLGRHEMERHGAGHERTFQTKPYEWGDPFNLHIEKTIRNAI
                     VRGESAPVRLRPEDFEIEETETLVRSATVLMLDLSLSMPMRDNFLPAKKVAMALHSLI
                     SMQYPRDYLGIVGFSEVARILTAEQLPEVSWDFVYGTNMQHGFQLARQLLARQSGTKQ
                     IIMITDGEPTAHINGYGEPEFHYPPIRATVDATLLEVARCTRERIRINTFMLDPDRSL
                     QHFVAKLTEMNRGRAFYTTPENLGDYVLVDFIEQKRELLRAGR"
BASE COUNT         1709 a         4148 c         4534 g         1936 t
ORIGIN      
        1 gccgcggtgg cgactccccg ccagtaccac gccgcgccga ccgcgaccac gagcagcacc
       61 ccgagcagga cggccacgcc ggtcatgagc agctccgcga caccgtcggc ctcaggacgc
      121 ctcgccaggc gccggcccgg agtcgggacc ggagtcggta ccgggtccga gcgcgtcgcc
      181 gtcggagccg ggcgacgggg tgatcggagc tgcggggcgg gtcgctccgg ggtgatcggg
      241 cagccagccg gtcaccatgt actggacccg ctcaccgatg ttgacggcgt ggtcgccgat
      301 ccgctcgtag taccggccga tcatggcgag ctgcaccgcg gcctgcaagt tcaggttgtc
      361 caccgagtgc gaggtgaaga tggcctggac gtacgcggtc tgcagctcgt cgaggcggtc
      421 gtcgatgtcg tcgagcgcgg cggccagcga cgtgtcggcg tcgacgtagg agtcgatggc
      481 ggtgcgcacc aggaacgccg cctcttcgcc catgtgctcg atgaggcccc gcagctgcgg
      541 cccgaactcg atgtcgtaga tgcgccgcga cgccttgcag acgttgacca tcaggtcacc
      601 ggagcgctcg agctctgaca ggagccgcaa cgtggtgaga atgaagcgga ggtcacccgc
      661 gatcggcgcc tgcaacgcca gcagctggca gcagcgctcc tcgaggccca ggcagaagtc
      721 gtcgatgacg tcgtcgccgt cgatcagcag ctgcgccgcg tgcaggtcgc ggtcgagcag
      781 cgcggcggtg ccccgaccga cggtctcgca caccatggcg cccagtcgga tgacgtcgga
      841 gcgcagcgcc tccatctcct cgtggaactc gattcgcatc tcatccatcg gacgtcccct
      901 cgggctcggt tgccgcggcc gcggccgtca gccgaaccga cccgtgatgt agttctcggt
      961 ccgctggtcg tgcgggttcg agaagatcgt ggccgtgcgg tcgaactcga ccagcagccc
     1021 ggtgcgcacg tcgctgtcgg ggttcacctc ggtcgagaag aacgcggtgg cgtcgctgac
     1081 ccgcgccgcc tgctgcatgt tgtgggtcac gatcacgatc gtgtagtccg tcttgatctc
     1141 cttcatcagg tcctcgatgc gggccgtggc gatcggatcg agcgccgagc acggttcgtc
     1201 catcaggatc acctcggggt ccaccgcgat ggcccgggcg atgcacagcc gctgctgctg
     1261 accgcccgac atgccgagcg ccgaggactt gagccggtcc ttgacctcgt cccacagcgc
     1321 ggcgcgctgc agggaccgct cgacgatgtc gtcgagctcg gagcgcttct tggtcccggc
     1381 gatccgaggg ccgtaggcga cgttgtcgta gatcgacttg gggaacgggt tgggcttctg
     1441 gaacaccatc ccgatgcggc gtcggacctc gaccgcgttg acgtcggggg cgtacaggtc
     1501 gacgccgtgg tagtcgaggc ggccctgcac ccgggccccc tcgatcaggt cgttcatgcg
     1561 gttgaagcac cgcagcacgg tggtcttgcc gcagccggac ggtccgatga agccggtgat
     1621 ctcgttgcgt cggatccgca tcgacacgtc gcggacggcc cggtagtcgc cgtagaacac
     1681 cgacaggtcc gacacgtcga acacgaccgg ggccagttcc ggctcggcga tcaccgacgc
     1741 ggcggcgagc ggttcgtgca ggtcgggggc gggggcgggg gcgaccgaca ctgacccggc
     1801 gccggggagg cctccggtgg ggacgagtcc ctcgggggtg acgactgcgt cggtggacat
     1861 cgggggctgc tcctcgtcgg tcatgtcggg ggtccggcgg cgtgacgtgt cccggggtgc
     1921 gccccgggtt cgcggtggct catcggagtg cgtagcggcg ggccacgaac cgggcgatga
     1981 gggtcagcac caacacgata ccgatcagcg tcaccgcggc cccccacgcc atttgggtgg
     2041 ccaggtcgcc gccgttctgc agctgcgagt agatctgcgc cgacagcgtg gtgttctgtc
     2101 cggtgaagcg ccagttggtg gtggtgacga aaccgatggt gaacacgatc ggggcggtct
     2161 cgcccgcggc gcgggccacc gcgagcaggc accccgacac gatcccgggc agcgccgcgg
     2221 gcagggtcac gccgacggtg gtcttccagg tgcgggtgcc gagcgcggcg gacgcctcgc
     2281 ggagcgggtc gggaaccagc cggagcatct cctcggtgga gcgcaccacg atgggaagca
     2341 tcaggcacgc cagggcgagc gacgccgcga acgcggactt gccctcgacg ccgaagcgca
     2401 gcacccagat cgagtagatg aacacgccca tgaccaccga cgggacgccg gtcatcacgt
     2461 cggtcatgaa ccggatgaag ttcgcgagcc ggttccgctt gccgtactcg ttgaggtaca
     2521 ccgctcccat gatccccagc gggatggcca gggccgacgc gccgaggacg gtcaggaagg
     2581 tgccgacgat ggcgggctgc atcccgagcg ccaccaagcc gtcggtgctg ctgtcggcgc
     2641 tgtcggtgct ggcggtgctg tcggggtcgg ccgcctggtc gccgaagccc aggtcctgga
     2701 gctgctcgtt gccggccagg tcggcccggc ccacgtccgc ggggatcggc tgggtccacc
     2761 agtcgaggtt ggtcaccgcc ggcagaccct ggctgaacac gttggcggcc atcaggacca
     2821 gcggtccggc cgccagcagg aacgcgacgg ccatcgccac ggtggccgcc cggttgcgga
     2881 cccggcgtcc gatcgagatg ttggcgtccg agagctcgcg tcgcacccgg tcgggggtcc
     2941 ggtcctcggg aacggtcgag gtggtgttca cgccgccccc ttcacccggc ggtcgatgac
     3001 gaccaccatc cggcgggccg acacgttgac caggatcgtc agcacgaaca gcacgacgcc
     3061 cagtccgatc agcgcggccc ggaacaggtc gtcggcctcg gacaggttgc ggaagatctg
     3121 cgccggcatg gtctcgccga cggcgaagat gttggcggtg atgtcggggc gggccccgat
     3181 cagcagggcg acggcgaccg tctcgcccat cgcccgaccc agcccgagca tgactgctcc
     3241 ggtcatgccg ccggtcgagt gggggaacac gacgccacgg atcatctccc agcgagtcgc
     3301 ccccaacgcc agggcgcccg ccttgtcgtt gggggggacg gtcgtgaaca cctcccgggt
     3361 gaccgaggtg atgatcgggg tgaccatcaa cgccagcacg agggccgccg acgcgtagct
     3421 cgacccggtc gagggaccga ggacggtgct gaggaccggg atgcccgaca cggcgtcgga
     3481 cacggcgttg aacgcggtct ggaacggtgg gatcaggtag tagaagcccc acaggccgaa
     3541 caccaccgac ggcaccgcgg cgagcaggtc gatggtggtg gtgaccgccc ctcgggcccg
     3601 tcggtgcacg acctcggtga cgaacagcgc gatgcccacg ctgatcggca ccgcgacgac
     3661 gagcgccagg accgacacca ggatcgttcc gaacaccaac ggcacgatcc cgtagagacc
     3721 ctcggacggc acccactccg tcccgaagaa gtagctgccc cccagctcgc tgaacgcggg
     3781 ccacgccttg ttggtggtga ccaccgcgat gagcagcagg gcgagcagca ccgtcaggcc
     3841 ggccgccagg gcgacgcgcc ggaacgagcg gtcgatccga ccgccgacgc cgtcggcggt
     3901 cagatcgggt gcgctccggt ccgcttccgg gtccggagcg gcgggcaggg tgtccagttc
     3961 gagggtcacg ggcacctccg gcggcggctc agccgccgat ctcctcgacc tggtcgtagg
     4021 ccttctgtcg gaggtcgtcg ggcagcccga cgaagccgag cttgcgggcc tgggcctggc
     4081 cggtggtcag caggtagcgc aggtaggtct tgagcgtggc ggcccgggcc gcgtcgggct
     4141 gggtccgggt gaccagcaga taggtcggcg cggtgatcgg gtagctgtcg gcgcccggtg
     4201 cgttcagcgg gttgtaggtc aggtcctcgg cgacgtccgc gttggcgacc gcggcttcgg
     4261 tggccgccgc ggacggagcc acgaaggatc cggccgcgtt cttgatcgac gcgaacgtga
     4321 gctcggcctt gatcgcgtcg gccaggtcga cgtagccgac gctgccgtcg gtctggccga
     4381 tgagggcggc gacgcccgag ttcttctcgg ccccctgggt ggccgccggc cagttcaccg
     4441 tgtcgcccga cccgagcgtc cacgtgtcgg gcgcggcggc ggagaggtac ttggtgaagt
     4501 tgttggtcgt gcccgatccg tcggagcggt gcaccacggt gatcttcgtc gccggcagcg
     4561 tggcgtcggg gttgtcggcc tcgatcgccg ggtcggccca gctggtgatc cggcccccga
     4621 agatcccggc gacggtgtcg gcgctgaggc ggagatcgtc gacgccggcc aggttgtagc
     4681 tgatggtgat cggtgccgcg acggtcggga agaacaggac ctgggagggg aacgtctcct
     4741 cgggcttggg cagcgagtcg cttccggcga cgtcgagcgt ccccgacgac aactgcttgc
     4801 gaccgtcgga cgatccgctc ttggcgtagg tgaccagttc ggagccggcg acgccgttga
     4861 agtcggcgtc gacagcctgg tagaaggcgt cggggaagct ggcgccgccc ccggcgacct
     4921 tggcggtcag cgcttcgatg tcggcgttcg acggggcggc gggtccggcg gcgccgctcg
     4981 tggtggacga gccgtcgtca gccgacgagc ccgactcctc ggaaccgccg cacgcggtgg
     5041 ccaccaggcc caccgccacg acgatcactg ccagcagccg gacgatccgg ctctcgtgca
     5101 acatcaacct gcctcctcgg aggtcgttcg gatcggtggc gtcgttgcca ccgatccgaa
     5161 acgtaggagt cgtcggtgtc gctcaggtgg ggtcgcggtg aacggagggt gaaccgcggc
     5221 tgaagcttcg gtcggtgctc gctgcgctcc gccggctggc gctggttcag acgtctgagc
     5281 ccgagcggaa ccgctcgagc accgtgcggt cggtgtccca gctcagcagt ccgtcgacgt
     5341 cgtcgagcgg gacccagcgg cgttcgtcgg tctcgtgacc gggccggaac ccgtcgtcgg
     5401 agtcgaccgt catcgcccac caccgcaccc gcttgtcctc gccgcgcggc gtcgggtagc
     5461 ggatctcgac caggcggtcc cccagggagc agcgcaggcc ggtctcctcc cacacctcgc
     5521 ggagcgccgc gtcgcggtgg cgttcgccgt cctcgagctt gcccttgggc agggaccagt
     5581 cgtcacgtgg cgccgggcgg tgcacgacga cgacctcgac cgcgccgcgg cggcgccgcc
     5641 acaccacccc tccggccgcc cggacgaccg gcgcgtccgg ggccggtccg ctccggctca
     5701 ccggcgtccg accgaggccc gcttctcggc gaactcctgc tgctcgaggt gcgtctcgac
     5761 ggtgttgacc cgcgggacgt gggtccagcg gtcgtcgacc tgctcccatg ccagggtgtc
     5821 gtcggccagc gcaacggcca gcacctcgtc catgcggacc cgcagctcgg gcgagcgcac
     5881 cggcaccacc gcctcgaccc ggcggtccag gttgcgtggc atcaggtccg cggagccgat
     5941 gaaatgcagc tcctggccgg ggccccgacc gttggcgaag cggtagatgc gcgagtgctc
     6001 caggtagcgc ccgacgatcg agcggacggt gatgttgtcc gacaggccgg cgacaccggg
     6061 gcggatgcag gagatccccc gcacgatcag ctcgacccgc acgccgtcct gggacgccgc
     6121 gtacagctcg tcgatcaggt ccgcgtcgac caggctgttc atcttcatga tgatgtggcc
     6181 ggtgccgggc gccgcggccc gctcgttgcg gatgaagcgg accagcccgt tgcgcagggt
     6241 gtgcggcgcc accagcagct tggcgtagtg gacgtctcgg gcgtagccgg tcaggtagtt
     6301 gaacagctgg gtgaggtccg cgcccaggtc ggggtccgcg gtgagcaggc cgacgtcctc
     6361 gtagaggcgg gcggtcttgg agttgtagtt gccggtgccg atgtggcagt agcggttgac
     6421 gctggtgccc tcgtcgcgga cgaccaggca cgtcttggtg tgggtcttga gtccgaccag
     6481 gccgtacacg acgtgcacgc cggcctgctc caggcggcgg gcccactcga tgttgcgctc
     6541 ctcgtcgaag cgggccttga gctcgaccag ggcgacgacc tgcttgccca tctcggcggc
     6601 cttgatcagc gcggcgacga tggggctgtc gccggaggtg cggtacagcg tgagcttgat
     6661 cgccagcacc ttggggtcgc gcgccgcctg gcggatgaac tcctcgaccg aggtggagaa
     6721 gctgtcgtac gggtggtgca ccatcacgtc gccctcgcgc atcaccgcga acaggtccgg
     6781 cggctcctcg tcctcgttga gggccaggcg gtgctgggtg accgggacga actcggggta
     6841 cttcaggtcg ggccggtcgg cgtcggccac cgcgaacagg ccgcccaggt cgagcggggc
     6901 ggcgtgcgcg ctgacgtcgt cgtcggtcag ctcgagttca tcgcggatca gctcgagcgc
     6961 ctcggcggag atgtcgtcct cgacctggag gcggaccgcc ttgccgaacc gtcggcggcg
     7021 cagctccatc tcgatggcgg cgaggaggtc gtcggcctcc tcctcctcga cggtgagatc
     7081 cgcgttgcgg gtgacgcgga atgcgacgtg gtcgaccacg tccatgcccg ggaacagctc
     7141 gtcgaggtgg tgcgcgatga cctgctcgag ggggaggaac cgctcgccgt cgggcatgac
     7201 gacgaaccgg ggcagcagcg acggcacctt gacccgggcg aagcggcgct ggtcggtggt
     7261 cgggtcccgc acgatgacgc ccaggttgag cgacaggctg gagatgtagg ggaaggggtg
     7321 gccggggtcg accgccagcg gtgtcagcac cgggaagatc cggttgcgga actcgacggt
     7381 ggcccactcg cggtcgtcgt cgtcgagctc gttccaggtc gagaagacga tgccctcctc
     7441 ggccaggttg cggcagatcg ggcccaggaa gatctcgtcc gcgtgggcga ccagctcggc
     7501 gctgcggcgg ttgatcgcct cgagctgctc ggacgcggac atcccgtcgg gactgcggcg
     7561 ggtgacgccg gccgccaccc ggtccttgag tccggcgacg cggacctgga agaactcatc
     7621 caggttctgg ctgaagatcg ccaggaactt gagccgctcg agcagcgggg tggccgggtc
     7681 ctcggccagg gccaggaccc gggcgttgaa ctccagccag gacagctccc ggttgaggta
     7741 gggcgagcgc tcgtccgggt ccgtcatggg ctcaggctac cggcccggat cgggccggga
     7801 gcgcccgaac cgggctcagc gctcgtcgtc gccgaggtcc cagaacagct cgatcagctc
     7861 gcgttcggcg tcgcgctcga cgcgctcgcg gttgcgcttg aactgcgggt cgtgcggggg
     7921 cagcagcatc agccgggcgt tgacccgggc ccggaagtcg tcgatcagct gggtgtcgcc
     7981 gtggtcgccg cggatctccc agtggggtgc gaccgggccg aggtagacga tggtgaccgc
     8041 cgccacccac tcgatgggct cgtcggactc ggaggcgtag gtgtcggaca tggggggcga
     8101 gcctaccggc tgcccgcctg aacgctctat cagcgccgcg ggcaactcag gaacaaatcc
     8161 gggcccccgg gcgtcagact ctccgtatgg atcaggaact ggacaccgag tggatggctc
     8221 acggcaactg cgccgatcag cccccgagcc tgttcttccc ctccgacggc gtcggggtcg
     8281 aggtcgccaa gcgcatctgc gcggactgcc ccatgagggt ccggtgcctg gactacgcgc
     8341 tcgacaaccg catcgaccac ggcgtgtggg gcggcaccag cgagcgtgag cggcgccgca
     8401 tcctcaaggc ccgccaggtc cgtcagcccg ccggcgtcgg ctgaggtccc gcaacgtcac
     8461 gacctcgacc cgctcccgcg ccggaaccca cggcgcgccg aagaaccccc cacccccgac
     8521 acggccgccc tccggggcgg ccgtcgtcgc ggtcaggccg acttcttgtc cgacgccttg
     8581 ggatcggtcg gagcgtcgtc gtcatcgtcc gcgctggcct cggccaggcc cttcttgaac
     8641 tccttctggg ccgagcccag gttgcgggcc agcttgggga gctgggaccc gccgaagatg
     8701 acgagggcga tgatcacgac gatcagccat tcgttgccac tgaacatgga ctcaccatac
     8761 cgttcgggcc cgtgacgtcc tcgtcggagc cgagcgcagc ggtgccctcg cccaatgacg
     8821 ccacccgccc cgtcgacccc gtcgagcccg tcgacccagt cgaccgggcc cggtcccacg
     8881 gccggaccgc ccgtgccgcg ctcgatcccc gggaacggga ggcggcgacc cgatcggtgg
     8941 tgacccgtct gtcggagctg gcagagctgg gcggacgcga gccgggttcc gtgccggggc
     9001 gggtggcgat cagcgtggcg gtgcgtggcg agctcgacgt caccggcgtc gccgccgtcg
     9061 tgcgcgaccg aggggttgag gtctggttgc ccgccgtcgt gggcgactcg ctcgtgttcc
     9121 gtgagtggcg cgacggcgac cggctggtcc cgggccggtt cgggatcccg gagccgcccg
     9181 acgacggccg cgacacgatc gacgcggcgg ggttggccgt cgtggtggtc ccgtgcgtcg
     9241 cggtcgacga gcacggcacg cgggtgggca gcgggttcgg gtactacgac cgggcgctgg
     9301 ccgacggctc tgacgccgga ccgactcgcc cgcggcgacc ggtcgtggtc gccgcggcgt
     9361 tcgaggtcca ggtggtcggc gagctaccga tgcgagcgtg ggacgtgccg gcggacgtcg
     9421 tcgtgaccga gcgacggacg atcctcaccc accgacgctg agcccgctca ggcggtgcgt
     9481 gactgctggg cggctcgggc cagcgcccgc tggcgacgga tgcggcgacg ctcccgctcg
     9541 ctcagtccac cccagatgcc gaacttctca ccgttggcga gcgcgtactc gaggcagtcc
     9601 tcgcgcacca cgcacccctg gcagacctgc ttggcctcct tggtggacgc cccccgttcc
     9661 gggaagaaca ggtccgggtc cacgcccagg cagttggcga acgcctgcca gctctcggcg
     9721 gcgacctcgt caacgccctc ggcggcggac tggttcggtt gggcgtcggt tgcgtcgctc
     9781 atgggttccc ctccggtccg ctccccccgg tcggggcgag cgatccctgc ggtcgtgtcc
     9841 agcccggccg cacctcgctg tggggccggg tggttcgttc gtgtcgttcg gtcggtgtgt
     9901 cggttctgtc aggtgatctc ggtgcccccc tcggacaggt cggaaccgga tcggcgtcga
     9961 ccgccccgtg ctctgaccgt cctgtgtaat tacaccgatg tagttgaaca tagtgacggt
    10021 ccccgcccga tttcgcaagc ggaacttggg gtttcacccg ccccggggcc cgggacggca
    10081 tctgcgcgac cgcccgtggg atccgcatcg tggtcgacgt tgctcccgcg ggatgccgac
    10141 cgcgtgccgg tcgctgtgct cagcgaccgg cgcgcagcag ctcgcgcttc tgctcgatga
    10201 agtcgaccag gacgtagtcg cccaggttct ccggcgtcgt gtagaacgcc cgcccccggt
    10261 tcatctcggt cagcttggcc acgaagtgct ggaggctgcg gtccgggtcg agcatgaagg
    10321 tgttgatccg gatgcgctcg cgggtgcagc gggccacctc gagcagggtg gcgtcgaccg
    10381 tcgcccggat cggcgggtag tggaactcgg gttcgccgta gccgttgatg tgggcggtcg
    10441 gctcgccgtc ggtgatcatg atgatctgct tggtgccgct ctggcgggcc agcagctggc
    10501 gggcgagctg gaacccgtgc tgcatgttgg tgccgtagac gaagtcccag ctgacctcgg
    10561 ggagctgctc ggcggtcagg atccgggcca cctcggagaa gccgacgatg cccaggtagt
    10621 cccgcgggta ctgcatggag atgagcgagt gcagcgccat cgccaccttc ttggcgggca
    10681 ggaagttgtc gcgcatgggc atggacagcg acaggtcgag catcagcacg gtggccgagc
    10741 gcaccagcgt ctcggtctcc tcgatctcga agtcctcggg ccgcaaccgc accggggcgg
    10801 actcgccgcg gacgatcgcg ttgcggatcg tcttctcgat gtggaggttg aacgggtcgc
    10861 cccactcgta gggcttggtc tggaaggtcc gctcgtggcc ggcgccgtgg cgttccatct
    10921 cgtggcggcc cagcaggtcg tggtcgagct tgcggaacag ctgggccagg gcgttctgcc
    10981 ccaggcggcg catgcccttg ggggtgagct ccaggcgacc ctcgcgctgt tcgaccaggc
    11041 cggcgtcggt gagcatcttg gtgatctcgc tcatccgctc caggctgtcg gcggcgttgt
    11101 cgccgagcag ctgccggacg cggtccaggt cgatgtcgcg cagcgctccg gggtcggtgc
    11161 cgccgcgcat catctgctcg agttggtcga ggtcgccgag ctggccgagc accgagctgg
    11221 cctgagccat gtcgagcggg tcgtcgccgc ggaagcggta gctggcgtcc cagtccaggt
    11281 cggggaacgc atcgcgcagg ttctggccga gctggtccat ctgccagcgc aggtccatgt
    11341 cctcgagcag ctggtccgac agctcctgga gctgggcgcg ttgctcgggg gtcatcgagt
    11401 tcatcagcgc ggccatggcc tgcatgcgcg ccgccatgat cgccagcagc tcgtcgaggt
    11461 tctcggggcc ctcggggaag aagtcgccga agcgctccat gaacccgtcg aagtcgggct
    11521 cctcgccgcg ggcccgggtc tcgagcatct ggttgagctc ggccagcatg tccttgaggc
    11581 gcgacatgtc ctgggggctc atgttctgca tggcgcccga catctggtcg accgcctgct
    11641 ggaccagctg ctcgcgcagg cggtccttga gcgcctcgaa gcgctgtgcg gcctcggctg
    11701 acgcgaagtc gtagtcgtcg aggcccctga ccttgccggc caggtccggc ggcagcatgt
    11761 cgagctcgag gttgcgcccg gcggcggact cctcggtcag ctcggtgcgg cgggcgtcgc
    11821 cggagtcgcg ggcggcctgg acgtcacgct cgagctgctc gcgctcctgg gcgacgacct
    11881 cgttgagctc gccggcgatg tcgtcgtaga cgccaccgag gtcgaagcgg tcgagcaggt
    11941 cctggcgctg ttcgcgcagc tgctcgagca tctcgcgcag gccctgcatc cgctcgccgt
    12001 tgcggtcctc gaagccctgc tgcatcatcc gccgcagcgc cgagttgagg tcgccgtggt
    12061 agagcaggtc gtcggtgagc tgctccatga cgtcgagggc gtcgagctcg aagccggtct
    12121 gggtgccgtc ccagggcgag tagcggaagc ggggacgggc catgcccgga ggctactgcg
    12181 cacccccgct ctgttccctt gcccccatcc cgttctgttc gttggatcac aacgcgagcg
    12241 ttgtgatcca acgaacagaa cggggggacg gggggggtgg tcaggcgcgg gcgcggtact
    12301 gggagcgtcc cccgacggcg tccttgt
//
LOCUS       DAOQOD010000091        12308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1815_length_12308_cov_13.323431, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000091 DAOQOD010000000
VERSION     DAOQOD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_12308_cov_13.323431"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..353)
                     /locus_tag="PLZ72_09900"
     CDS             complement(54..353)
                     /locus_tag="PLZ72_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36651.1"
                     /translation="MIAHLRSAAPDSTLSDALGGSTGLGALRLEAMATIGPHAADLID
                     PSGSVGDVIDQLDAAAQNSAAPQASGWAWSRVCLRPLADSDIEDLYQAALSTEGS"
     gene            complement(765..2036)
                     /locus_tag="PLZ72_09905"
     CDS             complement(765..2036)
                     /locus_tag="PLZ72_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36652.1"
                     /translation="MASDPGAAPTAGQAAELEYFKATYGFSDDRAAVAIEREAAAESL
                     LDKVGTLGLSSAWGGAWIDHSAGSTVHVGVTTSDAQKTLAALRLPDYVVVDQVRFSID
                     ELRKQRDEFRAAVVQELVKRAEETSQDSTRDLVAEAISGGWAANVYVDRNMVEIRVPK
                     GSPLSAAAQATKVADGRFEAVRVESVEGDISSRNSSSSTCADQIGDCFPLEAGIQIYG
                     ANGGGAYCTTGFTFIRSGTRYSSTASHCAGTPWKHHGHDIGPTAWTLEAHTVDAKMVS
                     ITDPNAWKATNLVFRPASSDIRVTSKISNPDNSLQGITVCQVGVGKANQGFNPISCGA
                     VTSIDGQSGSWKNPDWDQMGVATYRDCSGDSGGPVFNNSTGRAYGLVRGKVGSAPFPV
                     CTVGGQGGYESQFTWVSNIESASGYQVLLAQ"
     gene            2914..5613
                     /gene="mobF"
                     /locus_tag="PLZ72_09910"
     CDS             2914..5613
                     /gene="mobF"
                     /locus_tag="PLZ72_09910"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HNA36653.1"
                     /translation="MTTLKASAARVDAMLAYYVGLADDQESGCGHGPVDYYLDPDEPP
                     GRWRGQGRGALGLDGPVTGGDLRALLDGRHPRSGERLGRRFGDVSVRGFDATISAPKS
                     VSVLWALTPDPWVRAEVLAAHDAAVDAALGWFEHHGAVTRRGCDGIHQVDTAGITAAV
                     FRQHTSRTIDPQLHSHVVISAKVQDGTGTWLALDARFLKYQQRTIGWVYDAALRAELT
                     TRLGVGWHDRGDCVFDLDCVPESVREAFSERSTQVDAKLAELVRRWAAEHDDADPEPR
                     VLARLQRAAAVASRPAKVNGFDPAELHSHWTAEAHSAGFDPGRLTADRISQTSAPDAA
                     ITDEGLIGEALRRVADESATWLRADIARHLTTLVRPHAGADARGVVAEIDRLAAAAEQ
                     QCVTVGPNRDDATRRRRDGGPIAEHVTDRRLSLPTVLTEEQQLQEWAASATTPVALAG
                     DPQAAAARAIAGADRLVIVVGPAGTGKTHTTASAVQRLHADGRHVVGLAPSGKAADVL
                     ALEADCPTDTLAGFLTSHRTGRTPWPTGTTVILDEAGMAATTDLARLVDLVQQYRWRL
                     VVVGDPEQLPAVGRGGVFAHWSDTLPHLTLDTPRRFTDRWEAAASLGLRAGYSDAVAE
                     YANHGRLHTAHPATLAGQVARAHSHHTNAGRTVAITTTDAETARSINREIQWHAHPSA
                     RHAVALHDGTNAFVGDQITTRRNDPHLMTDYGQKVRNRHTWTVVAIRPGGGIAVTHPD
                     RGTAELPDAFVRANVELGWAVTGYGNQGDTVDIGIAVLDATTSRNHAYVAMTRGRHAN
                     HALLVDASGTIDPAERFTEILTRPISAESALATRARLHRQAGIGPPSTETTPDAPKPP
                     TRQPTGSPTDDPDLDRRRRAIQQRLDRIQRRPSNQPPDHSLGR"
     gene            6045..6275
                     /locus_tag="PLZ72_09915"
     CDS             6045..6275
                     /locus_tag="PLZ72_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36654.1"
                     /translation="MPNILVRDLPDDVHGALQQRAEKQGQSLQQYLTNELTRLVERPT
                     PAELFERVGRRSGGKVGLKRAVADLEGERPAS"
     gene            6272..6670
                     /locus_tag="PLZ72_09920"
     CDS             6272..6670
                     /locus_tag="PLZ72_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA36655.1"
                     /translation="MIVVDASVLANALADDGADGTAARARLAAAGELAAPDLVDVEAV
                     AVLRKRWIAGDLTGDRFSDAVEDLGDLAMTRYPALPLMRRAFELRDNVTAYDAAYVAL
                     AEQLGCTLLTADRRLAAAPTVTCEVEVLTT"
     gene            6971..7312
                     /locus_tag="PLZ72_09925"
     CDS             6971..7312
                     /locus_tag="PLZ72_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNA36656.1"
                     /translation="MSLVVVVVASPSRLANIPWSDVQPHLDVVANLLAGSLIGAWVGA
                     SIATTPRTSTLYRVLGVLLVLIAVVLVASHLLTVDGLGPDGPALVAVGVVAGVLIGLA
                     ASVMGVAGASC"
     gene            7506..7637
                     /locus_tag="PLZ72_09930"
     CDS             7506..7637
                     /locus_tag="PLZ72_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36657.1"
                     /translation="MLAGVVPEALLIPALGVLLVLSGYKVWHHELPAVDVEAAHHDG"
     gene            complement(7774..8034)
                     /locus_tag="PLZ72_09935"
     CDS             complement(7774..8034)
                     /locus_tag="PLZ72_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNA36658.1"
                     /translation="MFYEQAVMTCLDGMGLTSVHLASSMGEALDSISAAEFFASLEDA
                     LDVELPLSLMSECTTFGDIAAALAGTGSASASGRLGSNGSGS"
     gene            complement(8046..8702)
                     /locus_tag="PLZ72_09940"
     CDS             complement(8046..8702)
                     /locus_tag="PLZ72_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36659.1"
                     /translation="MPNGARLAGAEATAARALPAEGLPRFGQWALVRPVGDVTEMLLR
                     LAVAEQILWRWPSAPSVEGFYSVLDAPNSESYVIVDLASSAVVGHCGIYSFHAIHRTA
                     FFEASLVPELRYPHSMAGGVVLVALRNLLNRFDLRKLYFETTDARRDQFAFAEQRGYI
                     TEEARLRNHVFAEGTYKDVLILSAEASELRRALSEDQRLLHIVERGARSSETGTAEAR
                     "
     gene            complement(8707..9456)
                     /locus_tag="PLZ72_09945"
     CDS             complement(8707..9456)
                     /locus_tag="PLZ72_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36660.1"
                     /translation="MFVRNQRSTGRRVLLGAAAAASVLLLGACGDSAPPALSLHGGES
                     DADEIRFDTLETLVGQSAGVLRGRFRPDPVVVDRNVEEEADISEATYVWEFDVDKALL
                     NRYPKDSEVYAQIEKGGPIKVALLARDVDEQRGGKSFEEFHESRPSRYNFNSYPTDSD
                     VVIFTYPAGFPPDVLKKDSSLASAQVVAGGYACVDAKSLAGPCLYAADQPGGSEIGRI
                     AADELPKFSAGELEARLAAAPPEAVEPAGEG"
     gene            complement(9491..10180)
                     /locus_tag="PLZ72_09950"
     CDS             complement(9491..10180)
                     /locus_tag="PLZ72_09950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNA36661.1"
                     /translation="MRKRRSIAVSLTMGLTLKGLNPVAAGAHSWNNCPSNYAPTLRGP
                     GYISPSAPSGDPNVTYRLSPNTVLGVNDTARDQSRWALNTWGNQTDLNVTEITTTGWD
                     VYVTSYADPDQGNAGVTEPVIDNTPDYPPGGTNPSFFWCRFEHATVRWNTTHVNNAGN
                     WAGNDKGCIIAHEAGHAFGLGHSNIANHNDTGPPPGHTQATRSCGVESTRTAAMPRLL
                     RSGPEPGTSQT"
     gene            10788..11111
                     /locus_tag="PLZ72_09955"
     CDS             10788..11111
                     /locus_tag="PLZ72_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36662.1"
                     /translation="MLAGIVERLVSKDYAGLTADGLVSYSNDPADDSIGRWIEDYPAT
                     LVPLPEEAWEHGECGRFTDDPDAWWVVLDLWTAEEGRSDLSLEATVRESDDRVTVSVD
                     YVHVM"
     gene            complement(11209..11883)
                     /locus_tag="PLZ72_09960"
     CDS             complement(11209..11883)
                     /locus_tag="PLZ72_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36663.1"
                     /translation="MSRERWESETPFRWESDRDRVDAQWMSEKLAMGIEDTRESIDAL
                     TREVVDQQRLVDQLERLLDERRALLVQLRDLAVREREATAATRKAERQLAAERARTAV
                     RPDDGIRKRDGRLVRVKADDGAWRTVRAEAERRQVVMGVYLARLVDAEIDDETDRSLP
                     PSRRRRRSPGEGGEPKRVVHALRVFVTEDQWVPFRSCAAQLGLGLGVYVGELVEAEAR
                     RLGWRA"
     gene            complement(12026..12244)
                     /locus_tag="PLZ72_09965"
     CDS             complement(12026..12244)
                     /locus_tag="PLZ72_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA36664.1"
                     /translation="MKRSDVLSMTLTVSEAAGILGISRSSAYECVRRGELRAIRLGRR
                     LVVPRSAVLELLDGTRTAPTHAEPEAQP"
BASE COUNT         2020 a         4358 c         3945 g         1985 t
ORIGIN      
        1 accgcccgga gggggaccac cacccctcaa cttcaacagc tcccgggaca acctcagctt
       61 ccttccgtgc tgagcgctgc ctggtatagg tcctcgatgt cggaatcggc gagcggtcga
      121 aggcaaacgc gcgaccaggc ccagcctgag gcttgaggtg cagccgaatt ctgggccgcg
      181 gcatcgagtt ggtcgataac gtcgccgacg gatccgctcg gatcgatgag gtcggccgca
      241 tgggggccga tcgtcgccat ggcttccaga cgaagggcgc ccaacccggt cgagccccca
      301 agagcatccg agagggtgga gtccggcgcc gctgaccgga gatgcgcgat catggctcca
      361 tcaagcctcc gggtagggcg gccgtcttcg ccctgtgatt cacccatact ggtcgactat
      421 tcggaatctg ctgagggtcc agtcacctgc ttcatttcgg ctcagtgtca ggtcgacaag
      481 tgcgacatcg ccggtcatcg cctgctggag ttcctcgctc ttgtcgccac tcaaggtcgg
      541 attccggtca gcggtcgtcg ctccgcacgt cggagggtac gaagatccct gcgaatcact
      601 caagcagacg tgcagttcac cggcgtcgtc gcgttcaacc agggtggttg ccttgccttc
      661 gccgtccgag tagacagcct cgacgccagc gctcttaccc cctgaatcgc acgcgctcat
      721 gagcaagacg acgctgagag ctagacagac tccggtgggg cctttcattg cgccagcaac
      781 acctggtaac ccgatgcgga ctcgatgttc gagacccagg tgaactggga ctcataccca
      841 ccctggccac cgaccgtgca caccgggaac ggcgccgagc cgaccttgcc acgcacgagt
      901 ccgtaggccc ggccggtgga gttgttgaac actggccctc ccgagtcgcc gctgcaatcg
      961 cggtaggtgg cgactcccat ctgatcccag tcggggttct tccaactccc ggactgccca
     1021 tcgatcgagg tgactgcccc gcagctgatc gggttgaagc cctggttggc cttaccgacg
     1081 cccacttggc aaaccgtgat gccttggagg gagttgtccg ggttggagat cttcgatgtg
     1141 acccggatat cggaggacgc tggacgaaac acaaggttcg tagctttcca cgcattggga
     1201 tcagtgatcg aaaccatctt cgcgtccacc gtatgcgctt cgagagtcca agcagttggt
     1261 ccgatgtcgt gaccgtggtg cttccacgga gtccccgcac agtgtgaagc agtcgatgaa
     1321 tagcgcgtgc cggatcggat gaacgtgaaa cccgtcgtgc agtatgcgcc accaccgttc
     1381 gcgccataga tctggatccc cgcctccaac ggaaagcagt cgccgatttg gtccgcacag
     1441 gtggacgagg acgagttccg acttgagatg tccccctcca ccgactcgac tcgtacggcc
     1501 tcgaagcgtc catcggcaac cttggtcgcc tgggcagcgg cactgagcgg cgatcctttg
     1561 ggcacgcgaa tctcgaccat gttcctatcg acatagacgt tcgccgccca gcccccggag
     1621 atcgcctcag ccacgagatc cctcgtcgaa tcctgactgg tctcttcggc tcgcttgacg
     1681 agctcctgta cgaccgcggc ccggaactca tcgcgctgct ttcgcaattc gtcgatcgag
     1741 aagcggactt gatccaccac cacgtagtcg ggcagtcgca gggctgcaag ggtcttctgg
     1801 gcatcgctcg tggttacacc gacatgcacc gtggaccctg cagagtggtc gatccaggca
     1861 cctccccacg ccgacgacag cccgagggtc cccaccttgt ccagcaggct ctcggctgca
     1921 gcttcgcgct cgatcgcaac ggcggcgcgg tcgtcgctga acccgtacgt cgccttgaaa
     1981 tactccagtt cggctgcctg accggctgtc ggcgcagctc ccgggtccga ggccaccgag
     2041 ttctcagcgc ccgtcgaagg cgcctgggct accgccaggc ttgtgacacc aattgctaca
     2101 aatgcggact tcgtgataga tcgcaaggct cccctcccgc ttgctcggcc accgaccggc
     2161 ggcaccgagc cgcacgatag cagtgaagcc ggggtggttc cggtcctcag aggctccgct
     2221 cgaaacgcgc ctcgtcaact cccccgtcag cgatgagctg cggtgcagcg ccggatccgt
     2281 ggcgccataa caagctggcc gtttgcaccg ggccgccgcg cggcactccc caggccctcg
     2341 ctgccgggag ctcgcatgcg gatcacgtcg ccggcgtgta cccggatgat gcacttgtcg
     2401 gccagcgaca ccacgcggtc ccccctgggg cggcagcagg atcgtcgccc ctgccgcgcc
     2461 cgggctcccg ccggcctgac cctacggcgc actcacctgc cgttcggtga tgagccacac
     2521 cgtggccttg cccagcaccc cgcgagcgat cccgaagcag acacggccgt ccgcccaacg
     2581 atctcccgcc ggacctcccg ttcctctcgc ggcggcctcc cgcttccgga cgggacaccc
     2641 ctgtggcagc aaccgccgcg agcgtgtggt gagccgctcg aacgggaatc cagcgcctcg
     2701 ccggacgctc accatccttg gggacccgtc gccgaacctg ttgtcgagcg tccgcggtcc
     2761 cggtcgatcg tcgggaatcg ccggcgcccg cgaagttccg gtcgtgtgaa gggcctacgt
     2821 aagtgtggag cgacagacga gtgcattccc gccggcggga acaccagcgg gaggtcgatc
     2881 tgccctggtg ggtcggtggc cgacgtgatg cgcgtgacga ctctgaaggc gtccgccgcc
     2941 cgcgtcgatg cgatgctcgc ttactacgtc gggctcgctg acgaccaaga gtccggttgc
     3001 ggccacgggc cggtcgacta ctacctggac ccggacgaac cgcccgggcg ttggcgcggt
     3061 cagggccgcg gggccctcgg actggatggt ccggtgacgg gaggggacct gcgagcgctg
     3121 ctcgacgggc gccatccgcg gtccggtgag cggctcggtc gccggttcgg cgacgtttcg
     3181 gtccgagggt tcgacgcgac gatctcggca ccgaagtcgg tgtcagtgct gtgggcgctc
     3241 accccggacc cctgggtgcg tgccgaggtc ctcgccgcgc acgacgcggc ggtcgacgca
     3301 gcgctgggct ggttcgagca tcacggcgcg gtgactcgcc ggggctgcga cggcatccac
     3361 caagtcgaca cggcggggat cacggccgcg gtgttccgcc agcacacctc tcggaccatc
     3421 gacccgcagt tgcacagcca cgtggtgatc tcggcaaagg ttcaggacgg taccgggacg
     3481 tggctcgcgc tcgacgcccg gttcctcaag taccagcaac gcacgatcgg ttgggtctac
     3541 gacgctgcgc tgcgggctga gctcacgact cgactcgggg tcggctggca cgaccgcggc
     3601 gactgcgtgt tcgacctcga ctgcgtaccc gagtcggtcc gagaggcatt ctccgagcgg
     3661 tcaacccagg tcgacgcgaa gctggccgag ctggtccgac gctgggctgc tgagcacgac
     3721 gacgccgacc cggaacctcg cgtcctggct cggctgcaac gagcagcggc ggtggcgtca
     3781 cgccccgcca aggtcaacgg gttcgaccca gctgagctgc acagccactg gacggccgag
     3841 gcccactccg ccgggttcga ccccggccga ctcaccgccg accggatcag ccaaacctcg
     3901 gctcccgacg ccgccatcac cgacgagggc ctgatcggtg aggcgctcag acgggtggcc
     3961 gacgagtccg cgacgtggtt gcgagccgac atcgcccgtc acctgaccac cctcgtccga
     4021 cctcacgccg gcgccgatgc ccgcggggtg gtcgccgaga tcgaccgcct cgcggccgct
     4081 gccgagcagc aatgcgtcac ggtaggcccc aaccgggacg acgccactcg acgccgccga
     4141 gacggcggcc cgatcgccga gcacgtcacc gaccgacgac tctcgctccc caccgttctt
     4201 accgaggaac agcagctcca ggagtgggcc gccagcgcca ccaccccagt cgcccttgcc
     4261 ggcgaccctc aggccgcggc ggcccgggcg atcgccggag ccgatcggct cgttatcgtc
     4321 gtcggccccg ccggcaccgg gaagacccac acaacggcct ccgccgtaca acgacttcac
     4381 gcagatggac ggcacgtggt cgggctagcc ccctcaggca aggccgccga cgtgctcgcc
     4441 ctggaagccg actgcccgac cgacaccctc gccgggttcc ttaccagcca ccgcaccggc
     4501 cgtacgccgt ggccgactgg caccacggtg atcctcgacg aagcaggaat ggccgccaca
     4561 accgacctcg cacgactcgt cgacctcgtc cagcagtaca ggtggcgact cgtcgtggtc
     4621 ggggatcccg agcagctccc cgctgtcggt cgtggcggcg tgttcgccca ctggtctgac
     4681 accctcccgc acctcaccct ggacacgccc cgccgtttca ccgaccgatg ggaggccgct
     4741 gccagcctcg gcctccgggc cgggtactcc gacgcagtcg ccgaatacgc gaaccacggg
     4801 cgactccaca ccgcccaccc ggccaccctg gccggccagg tcgctcgagc ccacagccac
     4861 cacaccaacg ccgggcggac cgtggcgatc acgaccaccg acgccgagac cgcgcgctcc
     4921 atcaaccgcg agatccaatg gcacgcccac cccagcgccc gacacgcagt ggcgctgcac
     4981 gacggcacca acgcgttcgt cggagatcag atcaccaccc gccgcaacga cccccacctg
     5041 atgaccgact acggccagaa ggtccgcaac cgccacacct ggactgtcgt ggccatccga
     5101 cccggcggcg gaatcgcagt cacccacccg gaccgcggca ccgccgaact ccccgacgcc
     5161 ttcgtccgag cgaacgtcga actgggatgg gccgtcaccg gctacggcaa ccagggcgac
     5221 accgtcgaca tcggcatcgc cgtcctcgac gccaccacga gccgcaacca cgcctatgtc
     5281 gccatgacca gaggccgaca cgcgaaccac gccctcctcg tggacgcctc cggcaccatc
     5341 gacccggccg aacggttcac cgagatcctc acccgaccga tctccgctga atccgctctc
     5401 gccacccggg cacgactgca ccgccaggcc gggatcggac caccgtcgac cgagacgacc
     5461 cccgacgccc ccaagccgcc gacacgacag ccaaccggat caccgactga cgacccggat
     5521 ctcgatcgac gccgacgcgc gatccagcaa cgcctcgacc ggatccaacg acgaccgtcg
     5581 aaccagccgc cggaccactc cctcgggcgg taggcacgcc acccggaacc ggcgtccacc
     5641 tgggcacaat ctgggcacaa tcgggcacaa atcgcagcgg cacgagctgg tacgggccgg
     5701 tccaggagcc atacggcccg gcacggtcgc ctgggctcgg aaacgccgaa acccctggtg
     5761 tcgcagcgat ctcggctgcc caccaggggc ttcgatgctg gcacccccaa cgggattcga
     5821 acccgtgccg ccaccttgag agggcgacca aggtcgtccg acgggtccgg ccagtgcgat
     5881 tcgccccgtg tttccagggt tcaccgtccg gccggtccgg cgtcaacggt ccgatctcat
     5941 cgagtggatt accaagtgga ttaccaattc gcgattcagg cgacggcggc gctggtggtc
     6001 tctggcacct tgactagacc gcatgtcgat atcagaatgc aagcatgccg aacatcctgg
     6061 tgcgcgacct ccccgacgac gttcatggtg cgctccagca acgcgcggag aagcagggtc
     6121 agtcgctgca gcagtacctg accaacgagc tgacccgact cgtcgagcgg ccgacgcccg
     6181 cggagctgtt cgagcgggtc ggtcgtcgca gcggcggcaa ggttggcctg aagcgcgcag
     6241 tcgccgacct cgagggtgaa cgtcccgcgt cgtgatcgtc gtcgatgcat cggtcctcgc
     6301 gaacgcgctc gcggacgacg gagccgacgg caccgctgct cgcgcccgtc tggctgcggc
     6361 cggcgagctc gctgcacccg atctcgtaga cgtcgaagcg gtcgctgtgc ttcggaagcg
     6421 ctggatcgcg ggtgacctca ccggcgatcg cttctcggac gccgtcgagg acctcggcga
     6481 cttggcgatg acgcgctatc cggcgttgcc gctcatgcgt cgagcgttcg agctgcgcga
     6541 caacgtgacg gcctacgacg ctgcgtacgt cgccctggcc gagcagctcg gctgcaccct
     6601 tctcaccgcc gatcggcggt tggcggctgc tcccacggtg acctgtgagg tcgaagtcct
     6661 cacgacctga ttgccgaatt gcgcgagtgg ccggagcgta tggccggtga tgaaccgagt
     6721 tccggcactt cgctctgtct ggtcggtgct cctgagccgc gccttcggcg gcagaactcg
     6781 atgggtgggc tggtagacct gcggcccgtg gctggatcgc cgagatcgtc tgcaacggtc
     6841 gtggcggcgg gcgcgggcgt tgggttgctc ggcggtctga tcggactggg tggggccgag
     6901 ttccggttgc cgttgttgat cggcgtgttc ggcttcgcag ccttgagcgc cgtgatcctc
     6961 aacaagggcg atgagcctgg tcgtcgtcgt ggtggcgtcg ccgtctcgac ttgcgaacat
     7021 cccgtggtca gacgtgcagc cacacctcga cgtcgtcgcg aacctgctcg ctggctcgct
     7081 catcggcgcc tgggtcgggg ccagcattgc gacgacgccg cggacttcga cgctgtaccg
     7141 ggtgctaggc gtccttctgg tgctgattgc agtggtgctg gtcgcctcgc acctgttgac
     7201 cgtcgacggg ctaggacccg acggtccagc cctggtcgcg gtcggagtgg tcgccggggt
     7261 cctcatcggc ttggccgcgt cagtgatggg cgtcgccggg gcgagctgct gatccccacg
     7321 atcgtgctgc tgtacggcgt cgacattcgg atcgccggaa gcttgtcgct catggtgtcg
     7381 ctgcccacga tgatcgtggc gttcgctcga tatggccgtg acgacagctt catggtgttg
     7441 cgcgagaaca ggcggttcgt cgtgctcatg gctgccggat cgatcggcgg gaccgtcgtc
     7501 gggggatgct cgcgggtgtg gtgcccgagg cgctgctgat cccagcactc ggcgtgctgc
     7561 tcgtcctcag cggctacaag gtgtggcacc acgagctccc agcggtcgac gtcgaggctg
     7621 cccatcacga cggatagcgg caagtgctcg ccagccgccg ggtagccggc agctatgtcg
     7681 ccacttatcg ggatgcggtc cgaaggtcga ccgacgggaa ccggacaaga cgggcgagca
     7741 agtcgaacac acccgcgccg ctcgcatttc gtgtcacgag ccgctgccgt tggaaccgag
     7801 ccggcctgac gcagatgcgc tgccggtacc ggccagcgca gcagcgatgt caccgaaggt
     7861 ggtgcactcc gacatgagcg acagcgggag ctcgacgtcc agggcgtctt ctagcgacgc
     7921 gaagaactcc gccgcgctga tcgagtccag cgcctcgccc atggaggacg ccaggtgcac
     7981 gctggtcaga cccataccgt ccagacacgt catgacggcc tgctcgtaaa acacgctgcc
     8041 agtcgtcacc gggcctccgc agtgccggtc tccgacgatc tcgcgccgcg ctccacgatg
     8101 tgcagcagcc gctggtcctc gctcagggcc cggcgcagct cggaagcctc tgcgctcaag
     8161 atgagcacgt ccttgtaggt cccctccgcg aacacgtggt tgcggagtcg agcctcttcg
     8221 gtgatgtagc cacgctgctc cgcgaacgcg aactggtctc ggcgggcgtc ggtcgtctcg
     8281 aagtagagct tccgcaggtc gaagcggttc aggaggttgc gcagcgcgac cagcaccacg
     8341 ccgcccgcca tcgagtgcgg gtaccgcagc tcaggcacca ggctcgcctc gaagaacgca
     8401 gtccggtgga tggcgtggaa cgagtagatg ccgcagtggc ccaccactgc ggagctggct
     8461 aagtcgacga tgacgtagct ctcggagttc ggagcgtcga gcaccgagta gaagccctcg
     8521 accgatggtg ccgacggcca gcgccacaga atctgctcgg cgacggccag ccggagcagc
     8581 atctcggtga cgtcgcccac cgggcgcacc agcgcccact gcccgaagcg aggaagacct
     8641 tcagccggca gcgctcgcgc cgcagtggcc tcggcccccg cgaggcgtgc tccgttcggc
     8701 atccggttat ccctcgcccg ccggttcgac tgcctccggc ggagcagccg ccaacctggc
     8761 ctccagctca ccggcgctga acttcggcag ctcgtcggcg gcaatccgtc cgatctcgga
     8821 gccgccgggc tgatcagctg cgtacaagca gggcccggcg agcgacttgg catcgacgca
     8881 ggcgtacccg ccggcgacga cctgtgcgct cgccagcgac gagtcctttt tcagtacgtc
     8941 gggcgggaag ccagccgggt aagtgaaaat cacgacgtcg gagtcggtcg ggtagctgtt
     9001 gaagttgtag cgggacgggc gcgactcgtg gaactcctcg aacgacttcc cgccgcgctg
     9061 ctcgtccacg tcacgggcga gcagcgccac cttgatgggg ccgcccttct cgatctgtgc
     9121 gtagacctcg gagtccttcg ggtaccggtt caggagcgcc ttgtcgacat cgaactccca
     9181 cacgtaggtg gcttcggaga tgtcggcttc ctcctccacg ttgcggtcca cgacaacggg
     9241 atccggtcgg aagcggccgc gcaggacgcc ggcggactga ccgacgagcg tctcgagcgt
     9301 gtcgaagcgg atctcgtcgg cgtcgctctc acctccgtgc agcgacagcg caggcggtgc
     9361 gctgtcacca caagcaccga ggagaaggac cgatgcggcg gcggcagctc cgaggagcac
     9421 tcgacgtccg gtggaacgct ggttgcgtac gaacatgctg agtcccttcc gtccgctcag
     9481 tacttcgcgt tcacgtctga gatgtccccg gctcggggcc cgaacggagg agtcgcggca
     9541 tggcagcggt tcgggtgctc tccaccccgc atgaccgagt cgcctgcgtg tgtcccggcg
     9601 gcgggcccgt gtcgttatgg ttggcgatgt tgctgtggcc gagaccgaac gcgtggcctg
     9661 cttcgtgggc gatgatgcac cccttgtcgt tgccggccca gttgccggcg ttgttgacgt
     9721 gcgtggtgtt ccaccgcacg gtcgcatgct cgaagcggca ccaaaagaag ctggggttgg
     9781 tcccgcccgg tgggtagtcc ggcgtgttgt cgatcaccgg ttcggtcaca cccgcgttgc
     9841 cctggtcagg gtcggcgtag ctcgtgacgt agacgtccca gccggtcgtt gtgatctcgg
     9901 tgacgttcag atccgtctgg ttgccccacg tgttgagcgc ccaccgcgat tggtcgcgcg
     9961 cagtgtcgtt cacaccgagg accgtgttgg ggctaagccg gtacgtgacg ttcggatcgc
    10021 cactcggagc ggaaggggag atgtacccgg gaccccgcag cgtgggcgcg tagttgctcg
    10081 ggcagttgtt ccacgagtgg gctccggcag caaccggatt cagccccttc aaggtcaagc
    10141 ccatcgtcag gctgaccgcg atcgaacgtc gcttgcgcac agatccctcc tcgttcggca
    10201 gctgctccgg tgtgcaggag acctgcacct cggccagcga ccaccgcgtc ccatccgcgt
    10261 ggctgcaatc taagccggta agaagggccg atcaaggacc gctccggcgg ccgatcaagc
    10321 tggtggccgt tgtacggcat atgtcctgcg ggtcgacacg ggcatatgac ggccgccagg
    10381 cgtgttcggt tcccgctacg gcggcgccgt caacatgcga gccttcttcg gccagcgccg
    10441 atgcgatgag cactttcgtg atgcttccca atcaccttct cgcttcgcct caggacagac
    10501 ccggcactgc gtgcagccgg cacttcatga cgcacctgga gcgtccgatg cttgcatggg
    10561 aggcgaagcg cacagatcgc cgcttgggac gggcatcgca catcgggggc cgcatggaag
    10621 agcaagaatg cgctggatgc cggacgccgg tcaccgcatg gccgtgggtg tccgtcccac
    10681 aagacgacgg atcccgcctc gtgtaccacc agggctgcga gccatggcgg catccggatg
    10741 taccagccga cgatcctgtc aggccgatcc cgaatcggtg gcggccgatg ctcgctggga
    10801 tcgtcgaaag actggtctcg aaggactacg ccggactcac ggccgacgga ctcgtctcgt
    10861 actcgaacga tccagccgat gacagcatcg gcagatggat cgaggactat ccagccaccc
    10921 tggtgccctt gcccgaagag gcatgggagc acggcgagtg cgggcgcttc accgacgatc
    10981 cggacgcctg gtgggtcgtg ctcgacctct ggactgcgga ggaaggtcgc agtgacctga
    11041 gcctggaggc gacggtccga gagagcgacg acagggtgac cgtgtcggtg gactacgtcc
    11101 acgtcatgtg acctgaacga cacgggccga tacgtcgcgg gtctgacaga cgctcggtcg
    11161 acaacaggac cgccataggc ggtgaccatc ggcgaaggtg acgacgcgtc acgctcgcca
    11221 accgagccga cgagcctcgg cttcgacgag ctcgccgacg tagacgccga gaccgaggcc
    11281 gagctgtgca gcacaggagc ggaacggcac ccactggtcc tcggtgacga acaccctcaa
    11341 cgcgtgcacg acgcgcttcg gttcgccacc ctcgcccggg ctacgacgcc gacggcgact
    11401 cggaggcagt gacctgtccg tctcgtcgtc gatctcagcg tcgacgagcc gggcgaggta
    11461 cacgcccatc acaacctgtc gacgctctgc ctctgcacgc actgtccgcc acgccccgtc
    11521 atcggccttc acgcgaacca accgcccatc gcgcttacgg atcccatcat cgggccgaac
    11581 cgcggtgcgg gcccgctcgg ctgccagctg ccgctccgcc ttccgcgtcg ctgcggtcgc
    11641 ctcccgctca cgaaccgcca ggtcacgcag ctgcaccagc agcgcccgtc gctcatccag
    11701 cagccgttca agctgatcga cgagtcgctg ctgatccacc acctcgcgag tcaacgcgtc
    11761 gatcgactcc cgagtgtcct cgatgcccat cgccagcttc tccgacatcc actgggcgtc
    11821 gacacggtca cggtcgctct cccaacgaaa cggcgtctcc gactcccaac gctcccggct
    11881 cacgctcgcc tccgcagccg gcgcagcccg ctcttgcagc cgcgagttcc gtttgcggaa
    11941 ctcgccagtc tgtgccgtgc cttgaccgct cctcgaagca ctcgctgctc ggcctcgaac
    12001 gacctcgacg cgcgagtcgc cggggtcacg gctgcgcctc gggctcggca tgcgtcggcg
    12061 ccgtgcgggt gccgtcgagc agctcgagca ccgccgacct cggcaccacc aggcgccggc
    12121 ccagccggat cgcccgcagc tcaccccgcc gcacgcactc atacgccgac gaccgagaga
    12181 tccccaagat tcccgccgcc tcgctcaccg tcaacgtcat cgacaacaca tcacttcgct
    12241 tcacgcccgg accctcccgg cctcgtcacc accaggcgga catcacctgg catgggacct
    12301 aagcgcgc
//
LOCUS       DAOQOD010000092        12295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1818_length_12295_cov_19.467974, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000092 DAOQOD010000000
VERSION     DAOQOD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_12295_cov_19.467974"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2701
                     /locus_tag="PLZ72_09970"
     CDS             <1..2701
                     /locus_tag="PLZ72_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNA36665.1"
                     /translation="YNELFSSTVLPNHDGDHLTLPGLAGTFTPRHHQRAAVARILTDG
                     RALLAHAVGAGKTATMVMAAMELRRLGSASKPAVVVPNHMLDQFSREWLQLYPTARIL
                     VADRDRLSKARRKEFVARAATGDWDGIVFTQSGFARLPLGRDLMTDYLGEEIATARTA
                     LDGSKDGKGLSIKKLERRIAQMEETYKRLLSEHTKDDGVRFEETGIDYLFVDEAHAYK
                     NRRVDSSIDGVANTGSQRAQDLDSKLWALRRLHGPRVVTFATATPVANSMAELWVMQS
                     YLHPDLLASVDLRAFDAWAANFGRTHTALELAPDGASYRMQTRFARFQNVPELLTLYR
                     QVADVRTNEDLDLPVPSLAGGQAETVVVEPSDVLVDYVADLASRAERIRNRAVDPSED
                     NMLKVTGDGRRAALDLRLVGEDAPTDSGKLTVAAQRIAAIHHATRDHRYTDETNQLTL
                     RPGALQLVFCDVSTPAADGWNAYDELRALLVRRGVDHGSIRYMQDAKTDVAKAKLFAA
                     CRDGTVSVLIGSTETMGVGTNVQTRAIAMHHLDAPWRPADIEQRDGRILRQGNQNPEV
                     RVLRYVTEGSFDTYMWQTLERKAAFIAQVTRGDLPDRDVDDIGDQALSFAEVKALATG
                     DPLVLEKAAVDADVARLTRLERAHHDDQHRLRRTYETATLRAEKAEHRAGDLQTVVGR
                     VTDTRGERFAMTIDGRTHSKRVDAGEHVQSLLRRRLGQTAPETTGPTTEIGRLGGLAV
                     TAQAITTIEDEIRVAIPDAHIEVSYLAKDLTRHDAASLITRLERHLQRLPDTIADLNQ
                     EASSAKAEADRAAARIGVPWDRTEELVDLRRHQQEVEEQLAATTEPPIDQATSAPRAG
                     STETPPSAPPGGSAADRAAAARIAARLDIVQHRASTTTPGVEL"
     gene            complement(2951..3406)
                     /locus_tag="PLZ72_09975"
     CDS             complement(2951..3406)
                     /locus_tag="PLZ72_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36666.1"
                     /translation="MVSQSELADPSENESEPSEVLRLRALPAASQPAFPLEHDYFEIV
                     YTPLVGPTAVLLARAMARHLDAAGGPTTVCPIELAQEIGLRASSAKPLGKKSHLVHAI
                     DRLAHDHIVSRLEDRILGVRVAIPPVSAQTLAKLPVTVRDAHRRLVSVD"
     gene            complement(3412..3987)
                     /locus_tag="PLZ72_09980"
     CDS             complement(3412..3987)
                     /locus_tag="PLZ72_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36667.1"
                     /translation="MTAEQTRSTLLSAAADIFAAKGYDGASISDICTAASLTSGALYT
                     HYSSKAELFVAVLEAHGQRQFQDLVSGAGDVASFLATAGAAYGRRQTDSSLMIEAIVA
                     SKRHPEVAQLVASWLDADEDLLAASIREAQQSGALAEGLEPEAISRLATMISLGSILI
                     SELEVRNLDSEAWANLIDQLVDIVRVTRPGE"
     gene            4342..5169
                     /locus_tag="PLZ72_09985"
     CDS             4342..5169
                     /locus_tag="PLZ72_09985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNA36668.1"
                     /translation="MAFDEHLAQVPKHFAEDGDVLMSHFWAILSASFPEGEDFFVRSV
                     RHYRDQITDPVLKRQVAGFIGQEAMHGREHRVFNECLQALGYPVERMERLHRRMNALQ
                     ERLCSPETNLASTVVAEHFTALLAELVLTDESFRAVLGHQAVQDIYVWHALEESEHKA
                     VAFDVYRAMGGSERNRIWSMKLTRWALVLIAILIPVQVFVTDPAARRPGVLRRGVRRF
                     RSTSLMRKEMWEAIKDFEREDFHPEQRDTTALLDEWRERLFGDEGTLNDRLVGSTAV"
     gene            5184..6698
                     /locus_tag="PLZ72_09990"
     CDS             5184..6698
                     /locus_tag="PLZ72_09990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA36669.1"
                     /translation="MDRAVEHVDVLIVGAGLSGIGAAHHIATKTPWLTFAILEGRDAI
                     GGTWDLFRYPGVRSDSDMFTLGYGFRPWNGENSIADGASIRDYIRETAAEDGTDQRIR
                     FGHKVRTAAWSSDDALWHVTAELVSTGESVELTCRFLYSCTGYYRYDRGHLPEFPGYD
                     DFDGTIVHPQFWPDDLDYAGKRVVVIGSGATAVTLVPAMAETAGHVTMLQRSPTYIAS
                     APAKNPLNRGGPNGRWARSGLRWALAIGGVAGYQFSRRRPETVRKLLRKGVEKRLPEG
                     YDIDTHFTPAYDPWDQRMCMVPDGDLFTAISEGRASVVTDHIETFTPAGIRLASGEEL
                     EADVIVTATGLEMLFLGGIELTVDDEAVDPSTRLTYRGTMIEGVPNLAFAFGYTNASW
                     TLKCELSCDYVTRVLTRMRETGLRQCTPVHSGTEVEPLPFVDLTSGYVQRSVQSFPKQ
                     GDAFPWQVHQNYLRDYRAMRLKGLEDDVLRLSNPTPIHTSATPAGPALTGSRPR"
     gene            6695..7537
                     /locus_tag="PLZ72_09995"
     CDS             6695..7537
                     /locus_tag="PLZ72_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36670.1"
                     /translation="MKHFNGKVAAITGAGSGIGRALALELARRGCHLALSDIDEASLA
                     ETVVLCEGHGVKVTSCPVDVANRDAVFAWAEDVASEHGKVNLVVNNAGVSLGYDFQAM
                     SYDDFEWLMGINFWGVVHGTKAFLPHLLAADEGHVVNLSSVFGLISVPGQSAYNAAKF
                     AVRGMTESLRMELKGAHSTVSCTTVHPGGVKTNIVRNGRHDETRIAPGTDPAAAFEKI
                     ARTTPDQAAQTILRAVERDKRRVVIGTDGKIIDLLSRLPAAIYQGPLAFGARQAERRA
                     AKQG"
     gene            7740..8696
                     /locus_tag="PLZ72_10000"
     CDS             7740..8696
                     /locus_tag="PLZ72_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA36671.1"
                     /translation="MRVNTGGRVLAVALGVSAAVAIRRRRIRWSILDDPCGSEGLTLP
                     EGELFTLHTDDGADLAVLVAGDGPVVVLPHCWTGGMSIWAPVARGLVAGGHRVVLYDQ
                     RGHGSSTLGTGPISIDRLGLDLAVVLERLDVNDVVLAGHSMGGMTIQALAKVRSDVLA
                     GRVSGVVLASTAAHTGNLRVPPLAAKAFLGEARSRRLAKRPVSSDRRVVGAEVHRSHL
                     EATQAAMLATSGAARAGFLVAMSRMDYRPTLDNIQVPTRLLVGTRDALTPPRRARELN
                     DGIRAARLDVLPDYGHMLPLEAPEVLIATIEDLVRQTVTSFC"
     gene            complement(8810..9427)
                     /locus_tag="PLZ72_10005"
     CDS             complement(8810..9427)
                     /locus_tag="PLZ72_10005"
                     /inference="COORDINATES: protein motif:HMM:NF037005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36672.1"
                     /translation="MANDRPRRRRPEQARSRQRVQAILTAATELIAERGTEPPSMTEI
                     AERAGMGLTALYRYFPNKQSILRELSLQVLEFNRQSFVTPLVDSDAPIEELIRDSITA
                     YWHLHRDEPFRLRLRVAIHGDAELSALDLADSRQNALLFADQISQDGTDGYSSTVRHV
                     FLIISLLDSLMLTAIQLSREEADALVDDFADMAVSLLGGASRKSP"
     gene            9556..10899
                     /locus_tag="PLZ72_10010"
     CDS             9556..10899
                     /locus_tag="PLZ72_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA36673.1"
                     /translation="MGAGPAGLCMTRRLAETDARFTVLERNADVGGIWDIDAPGSPMY
                     ESAHFISSKTLSGFPDLPMPEDYPDYPNHRQILAYIRSYADRFDLRSHIQFDTGVVHA
                     ALDQDGSWQLELTDGRRVRARYLVCANGVTWEPNVISWPGRFDGEIRHSVTYRDASEF
                     AGKRVLVVGAGNSGVDIACDAAFAADQAFLSVRRGYHFIPKHIFGTPADVFAHNGPKL
                     PHWLEVRAFGVMLRLMNGDLTRLGLPKPDHRVFESHPLMNTQILHYLGHGDCIAKPDI
                     ASFDGPEVLFVDGSRERIDLVITATGYHHASPYLDEGVLEMRNGRPDLYLGMFARNHP
                     NLAVLGFIEFASAAYANYAAMAELIVADATAANGSTVQRRFADLKANHHPDLKGSHHY
                     VNSERHANYVDVDAYLRTIAQVKAQVQDGVGLSMAAEPRRGAVGSILRHRSVAGR"
     gene            10923..11714
                     /locus_tag="PLZ72_10015"
     CDS             10923..11714
                     /locus_tag="PLZ72_10015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36674.1"
                     /translation="MTGAASGIGLEVATRLHRRGHEVIAVDRNAVQLQAATSRIGGDT
                     VAIDCDLADPAQLALLIGELTTTWRESLDVVVCNAGVIAPGDLVDVDPATIDLQLDVM
                     LRSVIHLTRAVLPQFLERDRGHLLATVSMGGIIALPTSATYSASKAGLRAFLAAVNAE
                     VYATNVHVSGIYPSAVDTPMLLKEAEGGGSPLNFFGAVRSIDEVADAYERAIESHRLE
                     NYVPYSDSLTTRLLNAFPALTPKLLPIVNRIGERGRQRYLANHRH"
     gene            12226..12294
                     /locus_tag="PLZ72_10020"
     tRNA            12226..12294
                     /locus_tag="PLZ72_10020"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12255..12257,aa:Met,seq:cat)
BASE COUNT         2121 a         4299 c         3902 g         1973 t
ORIGIN      
        1 ctacaacgag ctgttctcct cgaccgtcct cccgaaccac gacggcgacc acctgaccct
       61 gcccggcctc gccggcacct tcacccctcg ccaccaccag cgcgccgcgg tcgcccgcat
      121 cctcaccgac ggccgcgccc tgctggccca cgcggtcggc gccggcaaga ccgccacgat
      181 ggtcatggcc gcgatggagc tacgccgact cggctcagcg tccaagcccg cggtggtggt
      241 cccgaaccac atgctcgacc agttctcccg cgagtggctc cagctctacc cgaccgctcg
      301 catcctcgtc gccgaccgag atcggctctc caaagcccga cgcaaggagt tcgtcgccag
      361 ggccgcgacc ggagactggg acggcatcgt gttcacccag tccgggtttg cgcggcttcc
      421 tctcggccgc gacctcatga ccgactatct cggcgaagag atcgccaccg ctcgcaccgc
      481 cctcgacggc tcgaaggacg gcaagggact ctcgatcaag aagcttgagc ggcgcatcgc
      541 ccagatggag gagacctaca agcggctcct ctccgagcac accaaggacg acggcgtcag
      601 gttcgaggag accggcatcg actatctctt cgtcgacgaa gcccacgcct acaagaaccg
      661 ccgcgtcgac tcctcgatcg acggcgtcgc caacacaggc tcccagcgcg cccaggacct
      721 cgactccaag ctgtgggcac tccgtcggtt gcacgggcct cgcgtcgtca ccttcgcgac
      781 cgccactccg gtggcgaact cgatggcaga gctgtgggtc atgcagagct acctccaccc
      841 cgacctcctc gccagcgtcg acctccgcgc cttcgacgcc tgggcggcga actttggtcg
      901 cacccacacc gccttggagc tcgcacccga cggcgcctcc taccggatgc agacgcggtt
      961 cgcccggttc cagaacgtcc ccgagttgct caccctctac cggcaagtcg cggacgtccg
     1021 cacgaacgag gacctcgacc ttccggtccc atcgctcgcc ggcggccagg ccgagaccgt
     1081 cgtcgtggaa cccagcgacg tcctcgtcga ctacgtggcc gaccttgcct cgcgtgccga
     1141 acgaatccgc aatcgggcag tcgacccgag cgaggacaac atgctcaagg tcaccggcga
     1201 cggccggcgg gccgcgcttg acctccgact cgtcggcgaa gacgcgccga ccgacagcgg
     1261 caagctgact gtggccgcac agcgaatcgc cgcgatccat cacgccaccc gcgaccatcg
     1321 ctacaccgac gagaccaacc agctcaccct ccgcccgggc gcgctccagc tcgtgttctg
     1381 cgacgtctcc acccccgccg ccgacggatg gaacgcctac gacgagctcc gcgccctgct
     1441 cgtccgccgc ggtgtcgacc acggctcgat tcgctacatg caagacgcca agaccgacgt
     1501 cgccaaggcc aagctcttcg ccgcgtgtcg cgacggcacc gtctcggtcc tgatcggatc
     1561 aaccgagacc atgggtgtcg gaaccaacgt ccagacgcgc gccatcgcca tgcaccatct
     1621 cgacgcgcca tggcgacccg ccgacatcga acaacgagac ggccgaatcc tccgacaagg
     1681 caaccagaac ccggaagtcc gcgtgctgcg ctacgtcacc gagggcagct tcgacaccta
     1741 catgtggcag acgctggaga ggaaggccgc gttcatcgcc caggtcaccc gcggcgacct
     1801 gcccgaccgc gatgtcgacg acatcggcga ccaagccctc tccttcgccg aggtgaaggc
     1861 gctcgccacc ggcgaccccc tcgtgttgga gaaggccgcc gtcgacgcag acgtcgcccg
     1921 tctcacccgg ctcgaacgcg cccaccacga cgaccagcac cgactccgcc gcacctacga
     1981 gaccgccaca ctccgcgccg agaaggccga gcaccgtgca ggtgaccttc agaccgtggt
     2041 cggtcgtgtg accgacaccc gcggcgaacg cttcgccatg accatcgacg ggcgcaccca
     2101 ctccaagcgg gtcgatgccg gcgaacatgt ccagagcctg ctccgacgcc gcctgggcca
     2161 gacggcaccc gagaccaccg gaccgacaac tgagatcggc cgcctcggcg gcctggccgt
     2221 caccgcccaa gccatcacca cgatcgagga cgaaatccgc gtcgccatcc ccgacgcgca
     2281 catcgaagtc agctacctcg caaaagacct cacccggcac gacgctgcca gcctgatcac
     2341 tcgcctcgag cgccatctgc aacgactccc ggataccatc gccgacctga accaggaagc
     2401 cagctccgcg aaggctgagg ccgatcgagc agcggcccgg atcggtgtgc cgtgggaccg
     2461 caccgaagag ctggtcgacc tgcgccgcca ccagcaggag gtcgaggaac agctggctgc
     2521 cacgaccgag ccaccgatcg accaagcaac gtcggcccct cgggccgggt cgaccgagac
     2581 tccgccatcg gcgccgcctg gtggctcagc agcggaccgg gcagcggcag cgcggatagc
     2641 ggcccggctc gacatcgtcc agcaccgggc gagcacgacc acaccaggag tcgagctgtg
     2701 acaagaccga gggagctggg ttcatcgaac tagcccgggg tcggcatgac tgagacatac
     2761 ggcatccgag gcacggctgc cccgcagctc ggcgcagtgg atcaccgcgg ggcggaaatg
     2821 tcgctcggga gagcgtcatc tgccgagtcc tcggcgagat ccggcagcga ctcgacagcg
     2881 tggatagatg tccgccaatg tcagtggcag ctggtgccag gacagggtct tcgcgggttt
     2941 cgtgggtcgt tcagtcgacc gataccaacc gtctgtgagc atccctgaca gtgaccggaa
     3001 gcttggcgag agtctgagca ctcacgggcg ggattgccac tcgcacccca agaatccggt
     3061 cttcgagtcg ggagacgatg tggtcgtgcg cgaggcggtc gatggcgtgg acgaggtgtg
     3121 acttcttgcc gagcggcttc gcgctgctcg cacggaggcc gatctcctgc gccagctcaa
     3181 tcggacacac ggtggtcggg ccgcctgccg catcgaggtg gcgtgccatc gctcgggcaa
     3241 ggagcaccgc tgtcgggcca acgagaggcg tgtacacgat ctcgaagtag tcgtgctcga
     3301 gcgggaaggc cggctgggat gcggcaggta gtgcccggag ccgcaagacc tcgctgggtt
     3361 cggactcgtt ctcggatgga tcggccagct cagactgcga gaccattcgg gtcactcgcc
     3421 tgggcgcgtc acgcgcacga tgtcgacgag ctggtcgatc agattcgccc acgcttcgga
     3481 gtcgaggttc cgaacctcga gttcagagat caggattgaa cccaggctga tcatcgtcgc
     3541 gagtcggctg atcgcctcgg gctccagccc ttctgcgaga gctccggact gctgcgcctc
     3601 gcggatggac gcggcgagca ggtcttcgtc ggcgtcgagc cacgaggcga cgagttgggc
     3661 gacctcgggg tggcgcttcg aggcgacaat ggcctcgatc atgagtgatg agtcggtctg
     3721 gcgccgaccg taggcggcac cagcggtcgc caagaagctg gcgacatccc cggcccctga
     3781 gaccagatcc tggaattggc gttggccatg ggcttcgagc actgccacga acagctctgc
     3841 cttcgagctg tagtgggtgt acagcgcccc gctggtcagg ctggcggcgg tacagatgtc
     3901 cgaaatgctc gccccgtcgt accccttcgc cgcaaagatg tccgcggcag ctgagagcaa
     3961 cgtcgaccgg gtctgctcgg cggtcacacc ggctctgcgt cccatcgatt cagttcccgg
     4021 tgtgggcgat gacagaggac gagacgtggc ctgccaggac ttgccttttg agcggcgaag
     4081 tcacgtcgac cgatcggcct gcccacggcg gcctctgcag caacctgggg tctggctcgg
     4141 gcaggtcagc ggcgtgggtc gtgatcatgg tcggggtcta cctccgagca acagctgctc
     4201 atcttccgtg tcatgccagc cgcaggggca tgggacttga aaatagtgat cgaccggttc
     4261 ttgcgaaaaa agtgatcgac cggtatgttg cccgacatga gtcagtcgag caaccccacg
     4321 cggaccgtcc cgacccgtcg gatggccttc gacgagcacc tggcccaggt tccgaagcac
     4381 ttcgcggagg acggcgacgt gctcatgagc cacttttggg cgatcctctc ggcgtcgttc
     4441 cctgagggcg aggacttctt cgtccggtcg gtccgccact accgcgatca gatcaccgac
     4501 cccgtgctca agcggcaggt cgccggcttc atcggccagg aggcgatgca cggtcgggaa
     4561 caccgcgtct tcaacgaatg ccttcaggca ctcggctatc cggtcgagcg gatggagagg
     4621 ctgcaccggc gcatgaacgc gctccaggag cggctgtgca gcccggagac gaacctcgcc
     4681 agcacggtgg ttgccgagca cttcaccgcg ctcctcgccg agctcgtgct gaccgacgag
     4741 tcgttccggg cggtcctcgg ccaccaggcc gtccaggaca tctacgtctg gcacgccctc
     4801 gaggaatccg agcacaaggc cgtcgccttc gacgtgtacc gcgcgatggg tggcagcgag
     4861 cgcaatcgca tctggtcgat gaagctgacg cgctgggcac ttgtcctgat agccatcctc
     4921 attccggtgc aggtgttcgt gaccgatccg gccgcccgcc ggcctggcgt gctgcgccga
     4981 ggcgtccgcc gattccggtc gacgtcgctg atgcggaagg agatgtggga ggcgatcaag
     5041 gacttcgagc gcgaggactt ccatcccgag caacgcgaca ccacggcgct gctcgacgag
     5101 tggcgcgagc ggctcttcgg cgacgagggc acgctcaacg accggctcgt gggctccacg
     5161 gcagtatgag cgtcgcgtcc ggcgtggatc gagcggtcga gcacgtcgac gtcctcatcg
     5221 tcggggccgg cctgtcgggc atcggtgccg cgcaccacat cgccacgaag accccctggc
     5281 tgaccttcgc gatcctcgaa ggccgcgacg ccatcggcgg cacctgggac ctcttccgct
     5341 accccggggt gcggtccgac tccgacatgt tcaccctcgg ctacggcttc cggccgtgga
     5401 acggcgagaa ctcgatcgcc gacggcgcgt cgatccgtga ctacatccgg gagacggcag
     5461 cggaggacgg caccgaccag cggatccgct tcggacacaa ggtccgcacc gcggcctggt
     5521 cgagcgacga cgccctgtgg cacgtcaccg ccgagctggt gtcgacgggc gagtcggtcg
     5581 agctgacgtg ccgcttcctc tactcgtgca ccggctacta ccgctacgac cgcggccacc
     5641 tccccgagtt cccgggctac gacgacttcg acggcacgat cgtgcacccc cagttctggc
     5701 ccgacgacct cgactacgcg ggcaagcggg tggtggtcat cgggagcggc gccaccgccg
     5761 tcaccctcgt cccggccatg gccgagacgg ccggccacgt cacgatgctc cagcgctcgc
     5821 cgacctacat cgcatccgca ccggcgaaga acccgctgaa ccggggcggg ccgaacggtc
     5881 gatgggcccg gtcggggctg cgatgggcgc tggccatcgg cggggtcgcc ggataccagt
     5941 tcagccgacg ccggccagag acggtccgca agctgctccg caagggcgtc gagaagcgcc
     6001 tgcccgaggg ctacgacatc gacacccact tcactcccgc ctacgacccg tgggaccagc
     6061 gcatgtgcat ggtccccgac ggcgacctgt tcacggccat ctccgagggt cgggcgtcgg
     6121 tggtcaccga ccacatcgag accttcaccc ccgccgggat ccggctcgcg tcgggcgagg
     6181 agctcgaggc cgacgtcatc gtcactgcca cgggactgga gatgctcttc ctcggtggca
     6241 tcgagctgac cgtggacgac gaggcggtcg atccgtcgac gcgcctcacc taccggggca
     6301 ccatgatcga aggcgttccc aacctcgcgt tcgcgttcgg ctacacgaac gcctcgtgga
     6361 cgctcaagtg cgagctgtcc tgcgactacg tcacgcgcgt gctcacgcgc atgcgggaga
     6421 cggggctccg ccagtgcacc ccggtgcaca gtggcaccga ggtcgagccc ctgccgttcg
     6481 tcgacctcac ctcgggctac gtccagcggt cggtccagag cttccccaag cagggggacg
     6541 cgttcccgtg gcaggtgcac cagaactacc tgcgtgacta ccgcgccatg cggctcaagg
     6601 gtctcgagga cgacgtcctc cgactgtcca acccgacacc catccacacc tcggccactc
     6661 cggccgggcc cgccctcacc gggagcagac cgagatgaag cacttcaacg gcaaggtcgc
     6721 cgcgatcacc ggcgcgggct cgggcatcgg ccgtgccctc gccctcgagc tcgcacgacg
     6781 tggatgccac ctggctctga gcgacatcga tgaggcgtcg ctcgccgaga ccgtcgtgct
     6841 gtgcgagggt cacggcgtca aggtgacgtc ctgtcccgtc gacgtggcca accgcgatgc
     6901 ggtgttcgcc tgggccgagg acgtcgcgtc cgagcacggc aaggtcaacc tcgtggtgaa
     6961 caacgccggc gtctcgctcg gctacgactt ccaggccatg agctacgacg acttcgaatg
     7021 gctcatgggc atcaacttct ggggagtggt gcacgggacc aaggcgttcc tcccccatct
     7081 gctcgcggcc gacgagggcc acgtcgtcaa cctgtcgagc gtgttcgggc tcatcagcgt
     7141 gcccggccag tcggcctaca acgccgccaa gttcgcagtg cgcggcatga ccgagtcgct
     7201 gcgcatggag ctcaagggcg cccacagcac cgtgtcctgc acgacggtgc atcccggcgg
     7261 cgtcaagacc aacatcgtcc gcaacggccg ccacgacgag acccgcatcg cccccggcac
     7321 cgatcccgcc gccgcgttcg agaagatcgc ccgcaccaca cccgaccagg ccgcccagac
     7381 gatcctccgc gccgtcgagc gcgacaagcg acgcgtcgtg atcggcaccg acgggaagat
     7441 catcgacctg ctcagccggc tccccgccgc gatctaccaa gggccgctcg ccttcggagc
     7501 acgccaggcc gaacgacgcg ccgccaagca gggatgagcg gcgtagccgg ttcccggctc
     7561 tatcgctccg acttcggtgc cgccacccac gcggatcgag ctgcctttgg cgaccggagt
     7621 cccgccgacg agttcaggtt ttcgacgctc gacagttcgt ccgccgcgct ggtcgtcagg
     7681 ccactgacgg acgggctcgt cgttcgagtc cgacctgacg tcgggaagga aggaggggga
     7741 tgcgggtgaa cacgggtggt cgcgtgctcg cggtcgcact cggcgtgagc gccgctgtcg
     7801 cgattcgtcg ccggaggatt cgctggtcga tcctcgacga tccttgtggg tcagaagggc
     7861 tcaccctccc cgagggtgag ctgttcactc ttcacaccga tgacggcgca gatctggccg
     7921 tgctggtcgc aggcgatggg ccggtggtgg tgctgcctca ctgctggacc ggcgggatga
     7981 gcatctgggc tccggtcgcc cgcgggctgg tcgccggcgg tcaccgagta gtgctgtacg
     8041 accagcgcgg tcacggcagc tcgacccttg ggaccggccc tatctccatt gatcgccttg
     8101 ggctcgacct cgccgtcgtg cttgagcggc tcgacgtcaa cgatgttgta ctcgctggcc
     8161 actccatggg gggcatgacg atccaggcac tcgccaaggt gagatccgat gtgctcgccg
     8221 gtcgagtcag tggtgtggtg ttggcctcca ccgccgcgca caccggcaac ctccgggtcc
     8281 ccccactcgc ggccaaggca tttctcggag aagcgcggtc tcgccgactg gccaagcgcc
     8341 cggtgtcgtc cgatcgacgt gtcgtcggtg ccgaggtaca ccgcagccac ctcgaggcca
     8401 cgcaggccgc gatgctcgcg accagtggcg cggctcgcgc cggatttctc gtcgcgatga
     8461 gccgtatgga ctatcggccc accctcgaca acatccaagt gccgacacgc ctcctcgtcg
     8521 gtacaagaga cgcgctgact ccgccgcgac gggcccgtga gctcaacgac ggcatccgag
     8581 cagcgcgact cgacgtactt cccgactacg gccacatgct gccgctcgag gcacccgagg
     8641 tgctcatcgc gaccatcgag gacctcgtgc ggcagacggt cacttcgttc tgctgaggga
     8701 cgtcatccaa gtcacggctg ccgccgcgac agcaccctgt caacggagac ccacgccgaa
     8761 gggtgtgccc caagatgctc ggtcggcctt tgttctgacg ggctgtccct catggagact
     8821 tgcgactagc gccgcctagc aaggagacgg ccatgtctgc gaagtcatcc acgagggcgt
     8881 ccgcctcctc tcggctgagt tggatcgctg tgagcatcag gctgtcgagc agcgagatga
     8941 tgaggaagac gtgacgcacg gtgctggagt aaccgtctgt gccgtcctga ctgatctggt
     9001 cggcgaagag cagtgcgttc tgccggctgt cagcgaggtc gagggcgctc agttccgcgt
     9061 cgccgtggat ggcaacgcgc aggcggagtc ggaatggttc gtcgcggtgg aggtgccagt
     9121 aggcggtgat cgagtcgcgg atgagctcct cgatcggagc atcggagtcg accagcggag
     9181 tgacgaagct ctgccggttg aactcaagca cctgaagtga cagctctcgc aggatcgact
     9241 gcttgttcgg gaagtaccgg tagagcgccg tgaggcccat gcccgcgcgt tcagcgatct
     9301 cggtcattga tgggggctcg gtgccgcgct cggcgatgag ctcggtcgct gcggtgagga
     9361 tcgcctgcac tcgctggcgg ctacgcgcct gctcgggccg gcggcgtctc gggcggtcat
     9421 tagccatggc tctttgacca tacagaagac gtgaggtatc ttcacgtccc atacgtgagg
     9481 agatctcacg tatggaaggg gtttgagttg gacactccag atacagccga tccggaactg
     9541 ctcgacgtgg tggtcgtggg tgcgggccca gccgggctct gcatgactcg gcgcctggcc
     9601 gagaccgacg cccggttcac cgtgctggaa cggaacgcgg acgtcggcgg gatctgggac
     9661 atcgacgcgc caggttcacc catgtacgag tccgcgcact tcatctcgtc gaagacgctc
     9721 tcgggcttcc ccgatctgcc gatgcccgag gactacccgg actaccccaa ccaccgccag
     9781 atcctcgcct acatccgctc gtacgccgac cggttcgatc tgcgcagcca catccagttc
     9841 gacaccggcg tcgtccacgc ggcgctcgat caggacggat cttggcaact cgaactgacc
     9901 gatggccgac gagtccgcgc tcgctacctg gtgtgcgcca acggcgtcac ctgggaaccc
     9961 aacgtcatct catggcctgg ccgtttcgac ggcgagatcc gccactccgt cacctatcgc
    10021 gatgcgagtg agttcgccgg caaacgcgtt cttgtcgtcg gggccggcaa ctccggtgtc
    10081 gacatcgcct gcgacgcagc cttcgccgcg gaccaggcgt tcctcagcgt gcggcgcggg
    10141 taccacttca tcccgaagca catcttcggc actccggcgg acgtcttcgc ccacaacggc
    10201 ccaaaactgc ctcactggct tgaggtgcgg gcgttcggcg tcatgctcag gttgatgaac
    10261 ggcgacctca ctcgcctcgg gctccccaaa cctgaccatc gagtgttcga atcgcatccg
    10321 cttatgaaca cacagatcct ccactacctc ggacacggcg actgcatcgc caagcccgac
    10381 atcgcgtcgt tcgacgggcc ggaagtcctg ttcgtcgacg gctcccgtga acggatcgat
    10441 ctggtcatca cagcgacggg ataccaccac gcgagtccgt atctggacga aggagtgctc
    10501 gagatgcgaa acggtcgacc cgacctttat ctcggcatgt tcgcacgcaa ccatccgaac
    10561 ctcgccgtgc tcggcttcat cgagttcgca tccgctgcgt acgcgaacta cgcggcaatg
    10621 gccgaactga tcgtcgcgga tgccacagcg gccaacgggt caaccgtgca gcgccgattc
    10681 gccgacctca aggcgaacca tcaccccgat ctcaagggca gtcaccacta cgtgaactcc
    10741 gaacgccacg ccaactacgt cgacgtcgat gcctacctgc gcacgatcgc tcaggtgaaa
    10801 gcgcaggtcc aagatggggt tgggttgtcc atggccgccg agcctcgtcg aggggccgtg
    10861 ggttcgatac tgcgacaccg ctcggtcgcc ggccgatgac cccgcggaga tctactgccg
    10921 tggtgactgg tgctgcgagc ggcatcgggc tcgaggtcgc cactcgtctc catcgacggg
    10981 gtcacgaagt tatcgctgtc gaccgcaacg cggttcagct ccaggcagcc acaagtcgta
    11041 ttggtggcga cacggtcgcg atcgattgcg acctcgccga tccggctcag ctggcactac
    11101 tcattggtga gctcactacg acgtggcgag aatcgctcga tgtggtcgtg tgcaacgcag
    11161 gcgtgatcgc ccctggcgac ctcgttgacg tcgacccggc caccatcgac cttcagctcg
    11221 acgtcatgct gcgaagtgtc atccacctca cgcgtgcggt actgccgcag ttcctcgaac
    11281 gggatcgcgg ccacctgctc gcgaccgtgt cgatgggtgg catcatcgcg cttcccacct
    11341 ctgcgacgta ctcagcatct aaggccggac tgcgagcctt tctcgcggcg gtgaacgcgg
    11401 aggtctacgc gacgaacgtg cacgtgagcg gcatctaccc cagcgcggtc gacaccccga
    11461 tgttgctcaa ggaagcggag ggaggtggga gcccgctgaa cttcttcggt gctgttcgca
    11521 gcatcgatga ggtggccgac gcgtacgaac gcgccatcga gagccatcgg ctcgagaact
    11581 acgtgcccta ttccgacagc ctgaccactc ggctgctcaa cgcattccct gcgctcaccc
    11641 cgaagcttct ccccatcgtg aatcgaatcg gcgaacgagg aaggcagcgc tacttggcga
    11701 atcaccggca ttgatcagac ctgcaggggt tggcgttgtg ggtcagccaa aagcgcgtga
    11761 gggcccccgc ggtcgtcgaa gttcgagtgg gcgcgtcggt cgccgtctac ggtcggagaa
    11821 aataccgacc tatgggcccc gagcgtatcg tcgcgatcga cgaggaacgc ctctgttcag
    11881 cggctcaccc gccccttacc gcccgctgcg aggtgctcgc cgaggtctgg ggcaaccgcg
    11941 cggccgtcta cccggcccac ggttgtgcgg caacggcggt cgatcatttc caccaccgat
    12001 gatccgagcg tcgaaggccg tcgctgggac ccgaaggtca ccgatgcatc gctccctccg
    12061 accccaaccg gcttctcgac gcgcgcgaga agcgacgccg aagttctacc ggaccttcta
    12121 ccgaaccccg cgttacagcc gatgatgggt gggcatgctg gcggacctcg ccccgccgaa
    12181 cctccacgtc agcgggacga tctgggacgg gctaggacga cgcggggcgg gtgcgtcaga
    12241 tagctcgtcg ggctcataac ccgaaggtcg caggttcaaa tcctgccccc gccac
//
LOCUS       DAOQOD010000093        12237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1835_length_12237_cov_11.316943, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000093 DAOQOD010000000
VERSION     DAOQOD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_12237_cov_11.316943"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLZ72_10025"
     CDS             complement(<1..791)
                     /locus_tag="PLZ72_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA36675.1"
                     /translation="MDAAEFLGLEPTHNPSRWYLPVTEGIITGGNFLFGGCGLGAAVA
                     AMERVCDRPLVWATAQYLSFATTGEIMDLDVTVAVSGRHTAQARAVGHVGDREILTVN
                     AALGSRELPVDGQWAVMPSVPEPEDCPVRAPRFGLERSIMTRLDVRIAVGSGWDALDG
                     NPVPDGRSALWVRVPDVEMSAAALSILGDYVPFGISQSLGGWYPGNSLDNTLRVVRLE
                     PSEWVLIDVRIDGLHSGFGHGSVHLWNQAGTLLATASQSTVVRNRR"
     gene            897..2279
                     /locus_tag="PLZ72_10030"
     CDS             897..2279
                     /locus_tag="PLZ72_10030"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HNA36676.1"
                     /translation="MLHSRSDIDDIENSLARLSKEIGRFEFPSEATDRRMVKEIIERE
                     IELDGNAHQNLATFCSTWESDEVKELMTASINKNLIDKDEYPMAAEIERRCVHMLADL
                     WNAPDSHDAVGTSAIGSSEACMLAGMAMHRRWRNRRRAEGKATDAPNLVCGPVQVVWH
                     KFARYWDIELREVGMAPGRLGLDPESVLGQVDENTIGVVPTLGVTYTGIYEPVADIAA
                     ALDDLQARTGLDVDIHVDGASGGFTAPFCAPDLVWDFRLPRVKSISSSGHKFGLAPLG
                     VGWVLFRDGSELPDELVFHVTYLGGDMPTFQINFSRPAGQIIAQYAGFLRLGREGYAA
                     VHGQSYATAHHLAQHLPAVGPFELLYDGDPAAGIPAATWKLADDADVTWTLYELADRL
                     RTRGWQVPAYPLTGELSGQVVQRALIRIEITTDEINSLITDMKEAVDFLERHPVTVQM
                     SQDEGTSFSH"
     gene            2381..2743
                     /locus_tag="PLZ72_10035"
     CDS             2381..2743
                     /locus_tag="PLZ72_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36677.1"
                     /translation="MTTQLTLISVPAAPSHPDADGVDRAEDGQGVSSAGPQRPGPAVS
                     SLGDRRTAAAPRRSAGHTGWLDRRTIASGRRGVAAARAALADAHRRVEERHSQEEADR
                     TDRLARQARELTDRGHAA"
     gene            2796..3974
                     /locus_tag="PLZ72_10040"
     CDS             2796..3974
                     /locus_tag="PLZ72_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HNA36678.1"
                     /translation="MTEVVIVDAVRTPIGRRNGGLSTVHPADLLGQVQSALIERTGLD
                     PAEVGQVVGGCVSQVGEQSFNIARTAWLAAGLPLGTASTTVDTQCGSSQQATNLATAL
                     VAGGVTDVAIGCGVESMSRIPIGANSSKKLGLGVPIPKPYFGRYEFTSQFEGAERIAE
                     RWGISRDDCDRFGLASQERAARAWAEDRFAGQLVSVTAPDVDADGNPTGTTHVVDRDE
                     GLRETTLDKLASLKPVAREDGVHTAGSASQISDGAAAVLVMTADRAGSLGLTPRARIV
                     DTCLVGVDPVLMLTGPIDATRRLLERTGLGIDDIDLFEINEAFASVVLAWAKELGADL
                     ERTNPNGGAIALGHPLGATGAFLLTKALDELERTGGRRALVSMCCGGGLGTGTIIERI
                     "
     assembly_gap    3991..4090
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4091..4523
                     /locus_tag="PLZ72_10045"
     CDS             <4091..4523
                     /locus_tag="PLZ72_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNA36679.1"
                     /translation="ANATVERVVDGDTLVVDIDGSRERVRLIGIDTPESVKPNSPVEC
                     FGKEASAHAAELLPDGTPVRLVLDVEERDRYDRVLAYVYRADDGLFVNLAMATDGYAG
                     VLTYPPNVAHTDQFRAAVADARSAGRGLWAACASDLPVESR"
     gene            complement(4553..>5385)
                     /locus_tag="PLZ72_10050"
     CDS             complement(4553..>5385)
                     /locus_tag="PLZ72_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA36680.1"
                     /translation="PTLAERLGYRSDDRLLIINCDDLGMCHSANEGVYDSLRNGLGTS
                     ATLMVPCPWAREAASQYRGEDVGVHLTLNAEWELYRWGPITVAPSLLDGDGGFPRTVV
                     DVWDHADLDETRRELRAQVERAILWGFDVTHLDSHMGTLQLRPEFFDIYLDLAVDFGL
                     PLRLTGWSTQDLTGFPHRALAEEEGIVAPDHLVFSAGPGVRPAFERLLDELRPGVTEI
                     FAHPATDHSELRSLAPDWETRVDDHHVLTGDVELRTMLERSGAVLIGWRELRDLQRKG
                     "
     assembly_gap    5386..5485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5547..6431)
                     /gene="mca"
                     /locus_tag="PLZ72_10055"
     CDS             complement(5547..6431)
                     /gene="mca"
                     /locus_tag="PLZ72_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HNA36681.1"
                     /translation="MTDPLRLLTVHAHPDDESSKGASSVAKYRAEGIPSTLVCCTGGE
                     AGDILNPAMDRPEVRDNLAEVRREELARATEAIGYETVEMLGYHDSGMPDMPENARPD
                     NFWNAPLDEAIGRLVAIIRRDRPQVIVTYPEDQSHYAHPDHLRVHDITVPAFDRAADP
                     AWYPEAGDPFQPLKLYYSTWSVARMSALHDKFIELGLESPFDESWRARWKDASQDHLI
                     TTQVPIRDFYAVREKALLAHATQIDPESPFWFGLPTDVAADVHPFDDYVLARSLVPTD
                     VPESDLFAGVPGRVREAL"
     gene            complement(6442..6825)
                     /locus_tag="PLZ72_10060"
     CDS             complement(6442..6825)
                     /locus_tag="PLZ72_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36682.1"
                     /translation="MVDPSASPRRRGTAVFAGLLGAGLLWVTATVVAALLAVGPVRSM
                     ACPSGGGFVHSDRVTLQVLPFGPTCRLEERDQVGAGLGTWVTVGPAPTWSRALLAGAV
                     TALVGAAGLVLAPRRRGHSPVRAHR"
     gene            complement(6917..8134)
                     /locus_tag="PLZ72_10065"
     CDS             complement(6917..8134)
                     /locus_tag="PLZ72_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNA36683.1"
                     /translation="MLAEAKDTSEIMVDLAYAAVYFNDPGMAEELGDLEEQLNDLVQE
                     MRAVCTLAVRRPAEAEGMASVLQVISAIEGIGNAAVDITRIVTHRLGIPNELIADLSN
                     ADEVSHRVWIREGSHLAHRPLKDLEIPVQCGMRVVAIRRGRSWMIEEVDGDVVVLPGD
                     VLFLRGSPAGITRLHELAAAPTWEPPEVPEPDALTDLDRAVDVLVEMKDVSEAAVGLA
                     YSALALRDTGLAAEVNHLAERLDEMKDHLQLWVLRAAKHDVDPSPLRGLLQLAGAAEE
                     IGNQAAEMVWLISDGVDVHPIVNIALGEADIVATRVPVAPGSVVDGRTLTELQLDIEP
                     GFWILAIRRDNRYLYQPRGWVRLAAGDDVIATGPHEGRERLAELCGWRMVEDEDDPTG
                     QIDLKPLRAAVTS"
     gene            complement(8270..9172)
                     /locus_tag="PLZ72_10070"
     CDS             complement(8270..9172)
                     /locus_tag="PLZ72_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA36684.1"
                     /translation="MTDTSTSPIRTDGGTEFESDTAVEALGDGRFRARISTTWWIVMG
                     PNGGYVAAIVLRAAVAAVADPARRPLSATFHYLRPPAEGEVDLDVTVERSGRGVTNVS
                     VRMSQGGKLIVLALVALGTRRDGTLEFDEVDSPTGSGLPTRPDGSSIPHWSEIPARPV
                     DPERDIPMRSRYDMRWGFGSVPFSGEPGSSADTGGWLRLKQSPPVDEVVLVAMADAWM
                     PPIFSRVDHQLAVPTVDLTVHFRGRPADPPGPPSGPEGPDAAWCFVRCSSPVSRGGYL
                     VEHGSVWDSTGRLLADVRQLAVVL"
     gene            complement(9267..10505)
                     /locus_tag="PLZ72_10075"
     CDS             complement(9267..10505)
                     /locus_tag="PLZ72_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36685.1"
                     /translation="MTMFEVPESVRPVRDRVEAFMRERVEPAEADLHRLADAAHHEPA
                     ARGELRERIRALQADAKAEGLWALGHPRELGGGGLPFMDYVYVNEVQGRSEYGQIALG
                     TYTLQDSIMLDRYASPEWRDAYLHRLVQSEVSPSFAMTEPEVASSDPTQLRTRAVLDG
                     DEWVIDGHKWFTTGANQAAYTTVMCRTEDDDVSPYLAYSMIIVPTDAPGYEIVRETPV
                     LGIRGGHCEVRLTGVRVPASNLLGPRGHGFVIAQERLGPGRIFHCMRWLGQAQRAFEL
                     MCTRLNTREAFGEPLAAKQLMQSHVFESYAEIQSCRLLTLHAAAAIDRGEQARVEIGA
                     IKVVGARMLHNVIDRAVQVHGAAGLTDDTPLSFMYRAARFGRIYDGPDEVHVHTVARR
                     ILKATRPAEDGTPRRWEFAR"
     gene            10636..11601
                     /locus_tag="PLZ72_10080"
     CDS             10636..11601
                     /locus_tag="PLZ72_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNA36686.1"
                     /translation="MSRNPILTDKAFGLDGSSGSGGLSPAQEWQRAQSMGAGPATGTV
                     PPSPPGYGVPPDATTAASAGTDAALARGGFGSLPTATDGKVMTMGGVAASTLVFFVFL
                     FVAAAFGWTSVTVTPDPLGRVDQNGNAIYDATMQRPWLLFVAMLVGLGLAFVTVFKPR
                     LAMFTGSAYALCEGYLLGAISAFYGAQFPGIVAQAIVATLGVFLMMLVLYGLRILRAT
                     PKFVKGVIAATFGIMVVYGVMLIANLFGVGEGFWTSGSPLGIVISLVVVTVAALNLIL
                     DFDFIERGQQMGLPRYMDWYAAFGLLVTLIWLYLEMLRLLARLRQ"
     gene            complement(11617..>12237)
                     /locus_tag="PLZ72_10085"
     CDS             complement(11617..>12237)
                     /locus_tag="PLZ72_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNA36687.1"
                     /translation="ITAQEVALRTSGPATPAAPSAGVPVAAQRPAPGAPAPTPTGPGP
                     TAPAPTPTTRPPTTPVTTVPPTAPSGGTGLVTWSDVTKAGGIWVNKSIAGQVSGLVAA
                     AKAAGINLSGGGYRDPAEQITLRRAHCGTSDYAIYWMPASQCHPPTAQPGRSMHERGL
                     ALDLMSNGRLITSRSDPAFVWLAANAGRFGFRNLPSEPWHWSTNGS"
BASE COUNT         1743 a         4262 c         4320 g         1712 t
ORIGIN      
        1 cgacggttgc ggaccaccgt cgactggctg gcggtggcga gcagcgtgcc ggcctggttc
       61 cacaggtgca ccgacccgtg gccgaacccc gagtggagcc cgtcgatgcg gacgtctatc
      121 agcacccatt cgctcggctc caggcggacg acccgaaggg tgttgtcgag gctgttgccc
      181 ggataccacc cgccgaggga ctgcgagatg ccgaacggca cgtagtcgcc caggatcgac
      241 agggccgcgg ccgacatctc gacgtcgggc acccgcaccc acagcgccga acgaccgtcg
      301 gggaccgggt tgccgtcgag ggcgtcccaa ccggatccga ccgcgatgcg cacgtcgagg
      361 cgggtcatga tcgaccgctc caggccgaag cgcggggccc gcaccgggca gtcctccggt
      421 tcgggcacgc tcggcatcac cgcccactgg ccgtcgacgg gaagctcgcg ggacccgagt
      481 gcagcgttga cggtcaggat ctcgcggtcg cccacgtggc cgacggcgcg ggcctgggcg
      541 gtgtgacgcc ccgacaccgc cacggtcacg tcgaggtcca tgatctcgcc ggtggtggcg
      601 aagctcaggt actgcgccgt cgcccacacc agaggccggt cgcacacccg ctccatggcc
      661 gccaccgccg cgcccaggcc gcagccgccg aacaggaagt tgccgccggt gatgatcccc
      721 tcggtcaccg gcaggtacca gcgggacggg ttgtgggtgg gttcaaggcc caggaactcg
      781 gcggcgtcca tggccgcgca ggctatcggc gcgccccgcc gccccacgac ggaccccggc
      841 cgcatcacct cgttggggtg aacccgtccc gcgggccgct cgggcatgct ggtgcgatgc
      901 tgcactcgcg ctccgacatc gacgacatcg agaactccct cgcccgcctg tcgaaggaga
      961 tcggtcgttt cgagttcccc tcggaggcga ccgaccgccg gatggtcaag gagatcatcg
     1021 agcgtgagat cgagctcgac ggcaacgccc accagaacct ggccacgttc tgctccacct
     1081 gggagagcga cgaggtcaag gagctgatga ccgcgtcgat caacaagaac ctgatcgaca
     1141 aggacgagta cccgatggcc gccgagatcg agcggcgctg cgtgcacatg ctcgcggacc
     1201 tgtggaacgc acccgactcc cacgacgccg tcggcacctc ggccatcgga tcgtccgagg
     1261 cgtgcatgct cgccggcatg gccatgcacc gccggtggcg caaccgacgc cgggccgagg
     1321 gcaaggcgac cgacgccccc aacctggtgt gcggcccggt gcaggtcgtg tggcacaagt
     1381 tcgcccgcta ctgggacatc gagctgcgcg aggtcgggat ggcccccggt cgcctgggcc
     1441 tcgatccgga gtcggtgctc ggccaggtcg acgagaacac gatcggtgtc gtcccgacgc
     1501 tcggcgtgac ctacacgggg atctacgagc cggtggccga catcgccgcc gcgctcgacg
     1561 acctccaggc ccgcaccggc ctcgacgtcg acatccacgt cgacggcgcc agcggcgggt
     1621 tcaccgcccc gttctgcgcc ccggatctgg tctgggactt ccggctgccg cgggtcaagt
     1681 cgatcagctc ctcgggccac aagttcggcc tggcacccct gggcgtgggc tgggtgctgt
     1741 tccgcgacgg ctcggagctg cccgacgagc tggtcttcca cgtgacctat ctgggcggcg
     1801 acatgccgac gttccagatc aacttctccc gtccggccgg tcagatcatc gcccagtacg
     1861 ccgggttcct ccggctggga cgcgagggct acgcggctgt gcacggccag agctacgcca
     1921 cggcgcacca cctggcccag cacctgccag ccgtcggtcc gttcgagttg ctctacgacg
     1981 gcgatccggc ggccgggatc cccgcggcca catggaagct ggccgacgac gcggacgtga
     2041 cctggaccct ctacgaactg gccgaccggc tccgcacccg cgggtggcag gtcccggcct
     2101 acccgctgac cggcgagctg tccggacagg tggtgcagcg ggcactgatc cgcatcgaga
     2161 tcaccaccga cgagatcaac tcgctgatca ccgacatgaa ggaggcggtc gacttcctcg
     2221 aacgccaccc cgtgacggtg cagatgagcc aggacgaggg cacctccttc tcacactgag
     2281 gctcgccccg atccgcgtcc gacggtctcg gcgcggagga tgctcagcgg ggaccagtcc
     2341 ccgtggacgc gttgtcacac cccctgttca tactggggcc atgaccacgc agctcaccct
     2401 catctcggtc ccggcggcgc cctcgcaccc cgacgccgac ggcgtcgacc gcgccgagga
     2461 cgggcagggc gtctcatcgg ccgggccgca gcgtcccggt cccgcggtca gttcgctcgg
     2521 cgaccggcgg accgccgccg cgccccgacg ctccgcgggc cacaccggtt ggctcgaccg
     2581 gcgcaccatc gcctcgggtc gacggggtgt ggccgccgcc cgggcggcgc tggccgatgc
     2641 tcatcgccgg gtcgaggagc gacacagcca ggaggaggcc gatcggaccg atcgtctggc
     2701 ccggcaggcg cgcgagctga ccgaccgtgg acacgccgcc tgaccggcca cggcccgccg
     2761 ccgtcccgcc gaccgccgct ccgtaggatg cacccatgac cgaagtcgtg atcgtcgatg
     2821 ccgtccgcac cccgatcggc cgccgcaacg gcggcctgtc gaccgtgcat cccgccgacc
     2881 tgctcggcca ggtccagagc gctctgatcg aacgcaccgg cctggatccg gccgaggtcg
     2941 gccaggtcgt cggcgggtgc gtcagccagg tcggcgagca gtccttcaac atcgcccgca
     3001 ccgcctggct ggccgccggg ctccccctcg ggaccgcctc gaccaccgtc gacacgcagt
     3061 gcgggtcgtc ccagcaggcc accaacctgg ccacggccct ggtcgccggc ggcgtcaccg
     3121 acgtggcgat cgggtgcggg gtcgagtcca tgagccggat cccgatcggc gccaactcct
     3181 ccaagaagct cggcctggga gtccccatcc ccaagccgta cttcggacgc tacgagttca
     3241 cctcgcagtt cgagggggcg gagcgcatcg ccgagcgctg gggcatctcg cgggacgact
     3301 gcgaccggtt cgggctggcc agccaggagc gggccgcacg ggcctgggcc gaggaccgct
     3361 tcgccggaca gctcgtgtcg gtgacggccc ccgacgtcga cgcggacggc aacccgaccg
     3421 ggaccaccca cgtcgtcgac cgggacgagg ggctccggga gaccaccctc gacaagctcg
     3481 cctcgctcaa gccggtcgcc cgcgaggatg gggtgcacac cgccggttcg gcgtcgcaga
     3541 tctccgacgg cgcggccgcg gtgctggtca tgaccgcgga ccgggccggg tcactcggac
     3601 tgaccccgcg ggctcggatc gtcgacacct gcctggtcgg cgtcgacccg gtgctcatgc
     3661 tgacgggacc catcgacgcc acacgcaggc tgctggaacg caccggtctc ggcatcgacg
     3721 acatcgacct gttcgagatc aacgaggcgt tcgcgtcggt ggtgctggcc tgggccaagg
     3781 agctgggcgc cgacctggag cgcaccaacc cgaacggcgg ggcgatcgcg ctcggccacc
     3841 cgctcggggc gacgggcgcg ttcctgctga ccaaggcgct cgacgagctg gagcgaaccg
     3901 gcggacggcg ggcgctcgtg tccatgtgct gcggtggcgg cctgggcacc ggcacgatca
     3961 tcgagcggat ctgagccgga cgccgtggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn ggccaacgcg accgtcgaac gcgtcgtcga cggcgacacg ctggtggtcg
     4141 acatcgacgg cagccgggag cgggtccgac tcatcgggat cgacaccccc gagagcgtca
     4201 agcccaactc gccggtcgag tgcttcggca aggaggcatc ggcgcacgcc gccgagctgc
     4261 tccccgacgg cacgccggtg cggttggtcc tcgacgtcga ggagcgcgac cgctacgacc
     4321 gggtcctcgc ctacgtgtac cgggccgacg acgggttgtt cgtgaacctg gccatggcca
     4381 ccgacggcta cgccggcgtg ctcacctacc cgcccaacgt ggcgcacacc gatcagttcc
     4441 gcgccgcggt ggccgacgcc cgctccgcgg gtcggggcct gtgggcggcg tgcgcgtcgg
     4501 acctgccggt cgagtcgcgg tagccccagc gccgttccgg gaggggtggg gctcagccct
     4561 tgcgctgcag gtcgcgcagc tcgcgccaac cgatcaggac cgcccccgac cgctccaaca
     4621 tcgtccggag ctcgacgtca ccggtcagca cgtggtggtc gtcgacgcgg gtctcccagt
     4681 ccggcgccag cgaccgcagc tcggagtggt cggtggcggg gtgggcgaag atctcggtga
     4741 ccccggggcg cagctcgtcg agcagtcgtt cgaacgcggg gcgcacgccc ggcccggccg
     4801 agaagaccag gtggtcgggg gccacgattc cctcctcctc ggccagcgcc cggtgcggga
     4861 acccggtcag atcctgggtc gaccagccgg tcagccgcag cggcagcccg aaatcgacgg
     4921 ccaggtccag atagatgtcg aagaactccg ggcgcagctg cagggtcccc atgtgggagt
     4981 cgaggtgggt cacgtcgaac ccccacagga tcgcccgctc gacctgggcg cgcagctcgc
     5041 gacgggtctc gtcgaggtcg gcgtggtccc acacgtcgac gacggtccgg gggaacccgc
     5101 cgtcaccgtc gagcagcgac ggggcgacgg tgatcggacc ccagcggtac agctcccact
     5161 cggcgttgag ggtcaggtgc accccgacgt cctcgccgcg gtactgcgat gcggcctcgc
     5221 gcgcccaggg gcacgggacc atcagcgtgg ccgaggtgcc cagtccgttg cgcagggagt
     5281 cgtagacgcc ctcgttggcc gagtggcaca tgccgaggtc gtcgcagttg atgatgagca
     5341 gccgatcatc ggagcggtag ccgaggcgtt cggccagcgt gggcgnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnatgat gagcagccga tcatcggagc ggtagccgag
     5521 gcgttcggcc agcgtgggcg cgtcgctcac agcgcctccc ggacccggcc gggcacgccg
     5581 gcgaacagat ccgactcggg gacgtcggtg ggcaccagcg agcgggccag gacgtagtcg
     5641 tcgaaggggt ggacgtcggc ggcgacgtcg gtcggcagcc cgaaccagaa cggggactcg
     5701 gggtcgatct gggtggcgtg agccagcagc gccttctctc gcaccgcgta gaagtcacgg
     5761 atcgggacct gggtggtgat gaggtggtcc tgcgacgcgt ccttccaccg ggcccgccac
     5821 gactcgtcga acggcgactc gaggccgagc tcgatgaact tgtcgtgcag ggccgacatc
     5881 cgggccaccg accacgtcga gtagtagagc ttgagcggct ggaacgggtc cccggcctcg
     5941 gggtaccagg ccgggtccgc agcccggtcg aaggcgggga cggtgatgtc gtggacccgg
     6001 aggtgatcgg gatgcgcgta gtgcgactgg tcctcgggat aggtgacgat gacctggggc
     6061 cggtcgcggc ggatgatggc gacgagccga ccgatcgcct cgtcgagcgg agcgttccag
     6121 aagttgtcgg gtcgggcgtt ctcgggcatg tcgggcatgc ccgagtcgtg gtagccgagc
     6181 atctcgacgg tctcgtagcc gatcgcctcg gtggcgcggg ccagctcctc gcgtcgcacc
     6241 tcggccaggt tgtcgcggac ctcgggccgg tccatggccg ggttgaggat gtcgcccgcc
     6301 tctcccccgg tgcagcagac cagcgtggag gggatcccct cagctcggta cttggcgacg
     6361 ctggacgcgc ctttggagga ctcgtcgtcg gggtgggcgt gcacggtgag caggcggagc
     6421 gggtcggtca cccgcaccac gctaccggtg ggcccggacc ggcgagtggc cgcgacggcg
     6481 gggtgcgagc accagcccgg ccgctccgac cagggccgtg accgcgccgg cgagcaacgc
     6541 ccggctccag gtcggggcgg gtccgacggt gacccaggta cccaggcccg ctccgacctg
     6601 gtcccgctcc tcgagccgac aggtcggtcc gaacggcagc acctgcagcg tcacccggtc
     6661 gctgtggacg aacccgccgc cggacggaca cgccatggag cgaaccgggc cgacggccaa
     6721 cagcgcggcg accaccgtcg cggtcaccca gagcaggccc gcaccgagca atccggcgaa
     6781 caccgcagtg ccccggcggc gcggcgatgc agagggatcg accatcgccg tccagggtgg
     6841 gcgcccagcc ggtcgctgtc gacgggacga tcccccagcc cgtcgaccgg ggcggccgac
     6901 gaggtgagga tcgacctcag ctcgtgaccg cggcgcggag tggcttcagg tcgatctgac
     6961 cggtcgggtc gtcctcgtcc tcgaccatcc gccagccgca cagctcggcc agtcgttcac
     7021 ggccttcgtg gggtccggtg gcgatgacgt cgtcgccggc ggcgaggcgg acccagccgc
     7081 ggggttggta gaggtagcgg ttgtcgcgac ggatggcgag gatccagaag cccggctcga
     7141 tgtcgagctg cagctcggtc agggtccgac cgtcgaccac cgagcccggc gccaccggca
     7201 cccgagtggc gacgatgtcg gcctcgccga gcgcgatgtt gacgatcggg tggacgtcga
     7261 cgccgtcgga gatcaaccag accatctcgg cggcctggtt gccgatctcc tctgccgcac
     7321 ccgcgagctg cagcaggccg cgcagcggcg acgggtcgac gtcgtgcttg gcggccctca
     7381 gcacccacag ctggaggtgg tccttcatct cgtcgaggcg ttcggccagg tggttgacct
     7441 cggcagccag gccggtgtcg cgcagggcga gcgccgagta ggccagaccg actgcagcct
     7501 cggagacgtc cttcatctcg acgagcacgt cgacggcccg atcgaggtcg gtcaacgcgt
     7561 cgggttcggg aacctcgggt ggttcccagg tgggcgcggc ggccagctcg tgcagacggg
     7621 tgatgccggc gggagaccca cggaggaaca gcacgtcgcc ggggagcacg acgacgtcgc
     7681 cgtcgacctc ctcgatcatc caggaccgac cgcgacggat ggcgacgaca cgcatgccgc
     7741 actggaccgg gatctcgagg tccttcagcg gtcggtgcgc caggtgcgag ccctcccgga
     7801 tccagacccg gtgcgacacc tcgtcggcgt tggacaggtc cgcgatcagc tcgttgggga
     7861 ttcccaggcg atgggtgacg atccgggtga tgtccaccgc ggcgttgccg atcccctcga
     7921 tggccgagat cacctgcagg accgacgcca tgccctcggc ctcggccggc cggcgaaccg
     7981 ccagggtgca cacggcgcgc atctcctgga cgaggtcgtt cagctgctcc tcgaggtcgc
     8041 cgagctcctc ggccatgccg ggatcgttga agtagacggc cgcgtaggcc aggtcgacca
     8101 tgatctccga ggtgtccttc gcctcggcga gcatggccct gagacttcga ggtcggtcgt
     8161 ccatatgtgg tcagccagga taggccccga cgcctcccca cgcgttctga cgacatctga
     8221 gtaccgccac ggtcgtgact tgtcgtcaga acgcggggcc tcgggggcgt cagaggacga
     8281 cggcgagctg gcggacatcc gcgagcaacc gaccggtcga gtcccagacc gatccgtgct
     8341 cgaccaggta gccgccccgc gacaccgggc tggagcagcg gacgaaacac cacgccgcgt
     8401 ccgggccctc cggcccgctc ggcggacccg gcggatcggc cggtcggccc cggaagtgca
     8461 ccgtcaggtc gacggtgggc accgccagct ggtggtcgac ccgactgaag atcgggggca
     8521 tccaggcgtc ggccatggcg acgagcacca cctcgtcgac cggaggcgac tgcttgagtc
     8581 gcaaccatcc gccggtgtcc gccgacgatc ccggctcgcc ggagaacggc accgagccga
     8641 acccccagcg catgtcgtag cgggaccgca tggggatgtc gcgttcgggg tcgacgggcc
     8701 gcgccgggat ctccgaccag tgcgggatcg acgatccgtc cgggcgcgtc ggcaggccag
     8761 acccggtggg tgagtcgacc tcgtcgaact cgagcgtgcc atcacgtcgg gtcccgagcg
     8821 cgacgagcgc caggacgatg agcttgccgc cctgggacat gcggaccgac acgttggtga
     8881 cgcccctccc cgatcgttcg acggtgacgt cgaggtcgac ctcgccctcg gcgggcggcc
     8941 ggaggtagtg gaacgtggcc gacagcggtc ggcgggcggg atccgcgacc gcggcgaccg
     9001 cggcgcgcag gacgatcgcg gcgacgtagc cgccgttcgg gcccatgacg atccaccacg
     9061 tcgtggagat ccgcgcccgg aagcggccgt cgccgagcgc ctcgaccgcc gtgtcgctct
     9121 cgaactcggt gcccccgtcg gtgcggatcg gcgaggtcga tgtgtcggtc atcggcgctg
     9181 ccctcccatc cgcgttctcg tgacagttga gtaccgccag tgtcgtgact tgtcgtcaga
     9241 acgcggggtg ggggccgggg gcggggtcat cgggcgaact cccagcggcg gggcgtcccg
     9301 tcctcggccg ggcgggtcgc cttgaggatc cgacgggcga cggtgtggac gtggacctcg
     9361 tcgggaccgt cgtagatgcg gccgaagcgg gcggcgcggt acatgaagct gagcggcgtg
     9421 tcgtcggtga gcccggcggc gccgtggacc tgcacggccc ggtcgatcac gttgtgcagc
     9481 atgcgggcgc cgacgacctt gatcgctccg atctcgaccc gggcctgctc accccggtcg
     9541 atggcggccg cggcgtgcag ggtcagcagt cgacaggact ggatctcggc gtaggactcg
     9601 aacacgtggg actgcatcag ctgcttggcc gccagcggct cgccgaacgc ctcgcgggtg
     9661 ttgagccggg tgcacatcag ctcgaaggcc cgttgggcct gacccaacca gcgcatgcag
     9721 tggaagatcc ggcccgggcc caggcgctcc tgggcgatga cgaagccgtg gccgcgcgga
     9781 ccgaggaggt tcgacgccgg gacgcggaca ccggtcaggc gcacctcgca gtggccgccg
     9841 cggatgccga gcaccggtgt ctcgcggacg atctcgtagc cgggagcgtc ggtcggcacg
     9901 atgatcatcg agtacgccag gtacggcgac acgtcgtcgt cctcggtgcg gcacatcacc
     9961 gtcgtgtagg cggcctggtt ggcgccggtg gtgaaccact tgtggccgtc gatcacccac
    10021 tcgtcgccgt cgagcaccgc tcgggtacgc agctgcgtcg ggtccgagct ggccacctcg
    10081 ggttcggtca tggcgaagct cggcgacacc tcggactgaa ccagccggtg caggtaggcg
    10141 tcacgccact ccggcgacgc gtatcggtcc aacatgatcg agtcctgcag cgtgtaggtg
    10201 ccgagcgcga tctggccgta ctcgctgcgg ccctggacct cgttgacgta cacgtagtcc
    10261 atgaacggca gaccgccgcc gccgagctcg cgcgggtgcc cgagcgccca caggccctcg
    10321 gccttggcgt cggcctgaag ggcgcggatc cgttcgcgca gctccccacg cgccgcgggc
    10381 tcgtggtggg cggcgtcggc cagccggtgc aggtccgcct cggccggctc cacccgctcg
    10441 cgcatgaacg cctcgacccg gtcgcggacg ggccgcaccg actccggcac ctcgaacatc
    10501 gtcatgacgc accccctggg atcgtcgaac ggaccccgat catggcctgc cccgaaccgc
    10561 ccggccgcca ccgctacctt tgaccgcagc ggatcggtcc ccctccggcc gccgaaacgt
    10621 gttcgagagg atgccatgtc acgcaacccg atcctgaccg acaaggcctt cgggctggac
    10681 ggatcgagcg gttcgggcgg tctgtcgccc gcccaggagt ggcagcgggc ccagagcatg
    10741 ggtgcgggcc cggccaccgg gaccgttccg ccgagccctc ccgggtacgg ggttccaccc
    10801 gacgccacca ccgccgcatc ggccggcacc gacgccgcgc tcgcccgcgg cgggttcggc
    10861 tcgctgccca ccgccaccga cggcaaggtc atgaccatgg gcggggtggc cgcgtccacc
    10921 ctggtgttct tcgtgttcct gttcgtcgcc gcggcgttcg gctggacctc ggtgacggtc
    10981 accccggacc cgctcggccg ggtcgaccag aacggcaacg ccatctacga cgcgaccatg
    11041 cagcggccgt ggctgctgtt cgtcgccatg ctcgtcggcc tgggtctggc gttcgtgacc
    11101 gtgttcaagc cccgcctggc catgttcacc gggtcggcct acgcgctgtg cgagggctac
    11161 ctgctcgggg ccatctcggc gttctacgga gcgcagttcc ccggcatcgt cgcccaggcg
    11221 atcgtcgcca ccctcggcgt gttcctgatg atgctggtgc tctacggcct gaggatcctt
    11281 cgggccaccc ccaagttcgt caagggcgtc atcgccgcga cgttcgggat catggtcgtc
    11341 tacggcgtca tgttgatcgc caacctgttc ggcgtcggcg aaggcttctg gaccagcggg
    11401 tcgccgctcg gcatcgtcat cagcctggtg gtcgtcaccg tcgcggcgct caacctgatc
    11461 ctcgacttcg acttcatcga gcgcggccag cagatgggtc tgccccgcta catggactgg
    11521 tacgcggcgt tcggactcct ggtcacgttg atctggctgt acctggagat gctgcggctg
    11581 ctcgcccgac tccgccagtg agctgacctg gtcggctcag ctgccgttgg tcgaccagtg
    11641 ccagggctcc gacggcaggt tgcggaaccc gaatcggccg gcgttcgccg cgagccacac
    11701 gaacgccgga tccgacctcg aggtgatgag gcggccgttc gacatcaggt cgagcgccag
    11761 gccccgctcg tgcatcgacc tgccgggctg cgccgtcggc ggatggcact gagacgccgg
    11821 catccagtag atcgcgtagt ccgaggtccc gcagtgggcc cgccgcaacg tgatctgctc
    11881 ggccgggtcg cggtagccgc ccccgctgag gttgatgccc gcggccttgg ccgcggcgac
    11941 cagcccggag acctgcccgg cgatcgactt gttgacccag atgccacccg ccttggtgac
    12001 gtcggaccac gtgaccagcc cggttccacc cgacggcgcc gtcgggggaa cggtggtcac
    12061 cggcgtggtc ggcggtcggg tggtcggggt cggggcgggt gcggtcgggc cgggcccggt
    12121 cggagttggg gccggagctc ccggcgcggg ccgttgagcg gcgaccggga cgccggcgga
    12181 cggggcggcg ggggtggcag ggccgctcgt gcgcagggcg acctcctggg cggtgat
//
LOCUS       DAOQOD010000094        12138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1855_length_12138_cov_11.259290, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000094 DAOQOD010000000
VERSION     DAOQOD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12138
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_12138_cov_11.259290"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLZ72_10090"
     CDS             <1..1033
                     /locus_tag="PLZ72_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNA36688.1"
                     /translation="ASSDRARRDGPAARTPAPEPPAPVAPRRAPAAPRRRRRWVRWVA
                     IGIPLLLLVGLATVTFRAFRAYSGIERVDLSAVLDPATGDTVNYLLVGSDSREGLDPD
                     VPVGGQSTVTGKRSDTIILLRVGPAGSQMMSIPRDLWVTVASTNRKGRINGAYNAGPA
                     NLVQTVKDNLKVPVNHYMEVGFESFAGVVDALGGITIDFPHPAFDTHSGLYVDQSGPV
                     TLDGRQALAYARSRYYTERIDGREVTDPTADLGRQQRQQQFLRTALGEAGGSRNPVEV
                     VAVTEALSSGLVLDDSLGLFDLIGLARSLSGTEPTTVVLPTEGARKGGAAVLVLDQPA
                     ADEVLAGFR"
     gene            1161..1712
                     /locus_tag="PLZ72_10095"
     CDS             1161..1712
                     /locus_tag="PLZ72_10095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNA36689.1"
                     /translation="MTDCPAADGSSPRQTRFDAELPMCIDAEKSYTAEIVTNHGTMRA
                     FLYPERAPRTVNNFVGLARYHYYDGLIFHRIIREFMIQGGCPEGSGRGGPGYRFADEL
                     PEPGRYEVGTLAMANAGPNTNGSQFFIVSGAHGVTLPPNYSIFGKLIDGFEVLSELEK
                     VRTGSGDRPVDDVVIESVTITES"
     gene            1726..3180
                     /locus_tag="PLZ72_10100"
     CDS             1726..3180
                     /locus_tag="PLZ72_10100"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNA36690.1"
                     /translation="MRRGAALTTVAVAAGAAAAVGTALVLRRNPVRAHQLRRTATMAR
                     MGARTGTNYVSMKARSALADQDRKQELNEEFQLRSAEQVAEMLGDMKGALMKIGQMAS
                     YLDQGLPEPLREALAELQQNAPPMAPELVEQVVRAELGDAPDRVFAEWDPVPLAAASI
                     GQVHRAVTRDGRAVAVKVQYPGVDRAVAADLESSDLLFQVLGMLFPGLDPAPIVTELR
                     ERLVEELDYRIEADNQRLFTDRYRDHPFIHVPAVFDDLSTARVLTTELVEGAPFSEVV
                     DWPDDERQLAAECLYRFAFGGIYQLHAFNGDPHPGNYIFHPGGRITFLDFGLCKRFSE
                     QEVADFEDMIQAMVLERDVARFRRVIERLGILSPELVVSDDELVAYFGHFYEFVMEPK
                     EMEITPEWSSDSVRRFFDLTGEHTDIMKAANLPPSMVIVQRINMGLFALFGDLHARAN
                     WREIAEEIWPFTDGAPSTPMGERIAEWEQTRTVA"
     gene            complement(3214..4983)
                     /locus_tag="PLZ72_10105"
     CDS             complement(3214..4983)
                     /locus_tag="PLZ72_10105"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNA36691.1"
                     /translation="MTRRDERSGPIRRRITPWRERNRRERGIAMVWFALTLLTMVLFA
                     GFAVDVSNWYFQAERIQRAADAGAHSGVIFLPADLPQATTVATGTVAKNGYPVGGPRN
                     TTVAVTQEPNPNRLRVKVTTDVPTFFLGLIGMDDVRMTREAVAEYVAPVPMGSPQNKL
                     GNDPDAVDPGTQLWVNISGPQTAKQQGDQYQSKVCGGSPREYGCTGTTNDQYETAGYL
                     YAMDVTSKGTGDLMFEVYDGVWTSNNLSCSQYMPTSSQINGGSGYTALTTKYPDAAAR
                     YSSATTAPANKYCTGDGHGNSSYGQVNTTFRFLQPDSTPWNDTDNPPVTQCPPVTING
                     FDPTKTSNSGRAIYDALMGSTINPNDGVLTYGETFRRWATLCKIPNGSVQPGKYIVQV
                     RTNVTAASPTTYNPSVSTAGHNHMAFRTGFGTAGVNAVNGSNVTVSALGRLPIYANAN
                     GADTRFYLAKVLPYDAGRTLRINLFDMGESSQPGSLQVLPPAEAYNGLTTFSGCAISR
                     DDGAGLSVNASTCTLSNVSSSSYNGRLLTIDVPIPTTYDCDEDSPTGCWIKIKAAYPS
                     SATVTDATTWSAAILGNPIRIVE"
     gene            complement(4980..5648)
                     /locus_tag="PLZ72_10110"
     CDS             complement(4980..5648)
                     /locus_tag="PLZ72_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNA36692.1"
                     /translation="MTNRTADSGRGAHGWRGSRLGRVYHHRTTRSDRGAALIEAAIIL
                     PVLMVFLLGIIEFGLVYATGATATGASRTGARLAVATFGPNKAAVTGSVQGPADLVAD
                     QVSGDLTALTSAVPIGMVIYKANSSGAPTGGYPSSTASMSGGCTADCMRYTWNTSTKK
                     FTPSGGPGWNLSTNDVCLPASTASVGVWVLIRHSYLTKLIGQYNFVEGRTVMRLEPLP
                     GDQC"
     gene            5744..6706
                     /locus_tag="PLZ72_10115"
     CDS             5744..6706
                     /locus_tag="PLZ72_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36693.1"
                     /translation="MPVDAELQPVLDLLAAMEQPPPMEAGPDAAREGFALLCAAFGPG
                     AQDVTATDITLAGADGPIPGRIYRPAGAAPSTTGAAGLVFLHGGGFVIGSLDTHDALC
                     RDLAAGADITVVSVDYRLAPEHPAPAAALDAASALDDVVRRAPELGLDPSRLAVGGDS
                     AGGNLAAVTAVAWRDRQRDDPGLAPLRLQVLIYPAVELADEDRFPSMKENAEGYLLTA
                     ETMAFFRHHYLTGATVDAADPMVSPLRTADLAGVAPALVLTAEYDPLRDEGEAYAAAL
                     GAAGVEVDLERFDGAIHAFVQMGSTGIGRQAVARICAALRDAVS"
     gene            6761..8380
                     /locus_tag="PLZ72_10120"
     CDS             6761..8380
                     /locus_tag="PLZ72_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA36694.1"
                     /translation="MTSTMGQVRVDERGGDAETASGPEGNGRAVGTASDGRTPGAATI
                     ETFDPRTGTLLAVVPDQTADEVRAAVARARAAGTAWSALTARERSRHMLAVRDRLLDR
                     AEELVRVICAETGKQAAEAVTTELMAVCETIDWYARNSERILRAQPVGSGTMAHKSAW
                     KRYEPLGVIGVISPWNYPFTLSMTPVITALFAGNTVVLKPSEVTPTVGLAIGALFEDD
                     TWGDVVQVVTGGGATGEALVRSGVDKIVFTGSVRTGRRVMAAAADTLTPVLLELGGKD
                     PMVVCEDADVVRAAKGAVWGSMQNSGQTCMSVERVYVHESVYDRFVSLVLSEVAAIRQ
                     DDHGGDIGSMTFEPQLQIIEAHLADALDKGATILAGGSRLDRDGLWFPPTVVVDVDHD
                     MDLMTEETFGPVLPIMKVSSDEEAVALANDSSYGLNSSVWTEDSDRGVHIANRLQAGN
                     VCINDCIVSYAVTGLPFGGVKSSGIGRVHGAEGLREMSIVKSILAPRVRTPRELWWFP
                     VPRNLDRIGIATLRARFGGNLRTKLGRRPGV"
     gene            complement(8387..>9052)
                     /locus_tag="PLZ72_10125"
     CDS             complement(8387..>9052)
                     /locus_tag="PLZ72_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNA36695.1"
                     /translation="GVRVDEARRVLGLDPDAPIDAKVLRKSFRRLVRVAHPDVNAGAD
                     AAERTTRLTLAYRVLSEAALRGESTTSVGARSSKDRPAPGGSGGSTPERGPAPSGDAA
                     SARRGDVPGPGREVPRIGVRLVGNDSISVSAPAEEALMLLIDAAHDLGEISYLDRTAG
                     LLEVIVEFVEAPTSSVLFSLQGRADGTTEVFCTVEALSGGDSPPAEAVTRLVLRTLRD
                     TQA"
     assembly_gap    9053..9152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9368..10306
                     /locus_tag="PLZ72_10130"
     CDS             9368..10306
                     /locus_tag="PLZ72_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA36696.1"
                     /translation="MAKQRAERDEEDLVRLYLSEIGQYPLLTKDDESRLAQAMEHGKE
                     AATELEANERSLSPARKRELRKLVKAGEEAERSFVQSNLRLVVSIAKKYQASGMPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQALTRGIANTNRTIRLPVHAGDNLA
                     RVQKARVRLETAYGRRPTVAELAADLDMPEDKVTEILRFAADTLSLSTPLREDGDAEL
                     GDIVEDPTAASPFEAAAESLLPAQISRLMMPLDDREREILSLRFGLDRGEPRTLEEVG
                     EHFDLTRERIRQIEARAMSKLRHPSSDTGARDLLNA"
     gene            complement(10426..11724)
                     /locus_tag="PLZ72_10135"
     CDS             complement(10426..11724)
                     /locus_tag="PLZ72_10135"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNA36697.1"
                     /translation="MTQDPTTVAEDLSDLIGFVDASPTPYHAVATAAALLDHAGFTAL
                     DPIEDWGPEAAGPAYVVRGGALVAWCPGRRSDPAAPLRMIGAHTDSPGLRVRPRPDRS
                     TAGWRQLGVELYGGALRNSWLDRDLGLAGRLAVRSDDAVELRRFRTDGAVMRVPQLAI
                     HLDRDVSTTGLVLNAQQHLTPVWGLGDASDGDFRAWLAALVGVEASAVVAWEALAYDV
                     QPSAVIGAERDMLAAPRLDDLAGCWAALTAVRHAASVMDPPELPTVVVLNDHEEVGSQ
                     TATGADGAWVEQVLERRVAALGGTRTDLLRSLTGSLLVSVDMAHATHPNYPERHEPGH
                     WIRPGGGPVIKHNVNARYATESTGAAAFRAACDRVGVPVQEYSHRGDLPCGSTIGPIT
                     ASRLAVDTVDVGAPMLSMHSARELMAVADITSLPTALQAI"
     gene            complement(11741..>12138)
                     /locus_tag="PLZ72_10140"
     CDS             complement(11741..>12138)
                     /locus_tag="PLZ72_10140"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNA36698.1"
                     /translation="VDTLVVFLHYGTETQLCPNARQKELVELLTGAGADIVVGSHPHR
                     LQGVGYHGQQFVAYSLGNFAFQANSPEGAATGVLMVTATGRRIDGYTWTPAVIRSSIP
                     HPLTGTAADAAQATMTQRQQCAGLTPQAS"
BASE COUNT         1765 a         4264 c         4230 g         1779 t
ORIGIN      
        1 ggcatcgagc gatcgggccc gtcgcgacgg ccccgccgcc cggacccccg caccggaacc
       61 gccggctccg gtcgcgccgc ggcgcgctcc cgccgccccg cgtcggcgac gacgctgggt
      121 caggtgggtc gccatcggga tcccgctgct gctgctcgtc ggcctggcga cggtcacgtt
      181 cagggcgttc cgcgcctact cggggatcga gcgcgtcgac ctttcggcgg tgctggaccc
      241 ggcgaccggc gacacggtga actacctgct cgtcggttcc gattcgaggg agggtctcga
      301 ccccgacgta ccggtcgggg gacagagcac cgtcaccggc aagcgcagcg acacgatcat
      361 cctgctgcgg gtcggtccgg ccggcagcca gatgatgtcg ataccccgcg acctgtgggt
      421 caccgtcgcg tcgaccaacc ggaagggccg catcaacggc gcctacaacg cgggtccggc
      481 caacctggtg cagaccgtca aggacaacct gaaggtgccg gtcaaccact acatggaggt
      541 cggcttcgag agcttcgccg gtgtcgtcga cgcgctcggc ggcatcacga tcgacttccc
      601 tcaccccgcg ttcgacaccc actccggcct ctacgtcgac cagagcggtc cggtcaccct
      661 cgacggccgc caggccctcg cgtatgcgag gtcccggtac tacaccgagc ggatcgacgg
      721 gcgcgaggtg accgacccca ccgcggacct cggccgacag cagcggcagc agcagttcct
      781 tcgcaccgcg ctcggcgagg ccggcggaag tcgcaacccc gtcgaggtcg tggcggtgac
      841 cgaggcgctg tcgtcaggcc tggtgctcga cgactcgctc ggcctgttcg acctgatcgg
      901 gctggcccgg tccctgagcg ggaccgagcc gaccaccgtc gtgctgccca ccgagggagc
      961 ccgcaagggc ggtgcagccg tgctggtgct cgaccagccg gcggccgacg aggtcctggc
     1021 gggcttccgc tgaccgaccg ccccggtgcc agccccgtcg ctcgggcccg ggggcgctcg
     1081 acgggtaggg tgcgccggtc ccgaaccggt cacgacgcgg tcgtccggac ccaccccgac
     1141 cccaccccca ggagatcccg atgaccgact gtcccgccgc cgacggctcg tcgccccgcc
     1201 agacccgctt cgacgccgag ctgcccatgt gcatcgacgc cgagaagagc tacacggccg
     1261 agatcgtcac caaccacggg accatgcggg cgttcctgta ccccgagcgg gcgccccgca
     1321 cggtcaacaa cttcgtcggc ctggcccgct accactacta cgacgggctg atcttccacc
     1381 ggatcatccg tgagttcatg atccagggcg gctgccccga ggggtccgga cggggcggtc
     1441 ccggctaccg gttcgccgac gagttgcccg agccgggccg ctacgaggtc gggaccctgg
     1501 ccatggccaa cgcaggaccg aacaccaacg gcagccagtt cttcatcgtg tccggcgccc
     1561 acggcgtgac cctgccgccg aactactcga tcttcgggaa gctcatcgac ggcttcgagg
     1621 tgctgtcgga gctggagaag gtccgcaccg gctcgggtga ccgtccggtc gacgacgtcg
     1681 tgatcgagtc ggtcacgatc accgagagct gacggtccgg cgaccatgag gcgcggagcc
     1741 gccctcacca cggtcgcggt cgccgccggc gcggcggccg ctgtcggcac cgcgttggtg
     1801 ctgcgacgca acccggtgcg ggcccaccag ctgcggcgca ccgcgacgat ggcgaggatg
     1861 ggcgcccgca ccggcaccaa ctacgtgtcg atgaaggcgc gttccgcact ggccgaccag
     1921 gaccggaagc aggagctcaa cgaggagttc cagctgcgca gcgccgaaca ggtcgccgag
     1981 atgctcggcg acatgaaggg cgcgttgatg aagatcggcc agatggcgag ctatctggac
     2041 cagggcctgc ccgagccgct ccgagaggcg ctcgccgagc tccagcagaa cgcgccgccc
     2101 atggccccgg agctggtcga gcaggtggtc cgggccgagc tcggcgacgc acccgaccgg
     2161 gtgttcgccg agtgggaccc ggttccgctc gccgcggcgt cgatcggaca ggtgcaccgc
     2221 gccgtcaccc gggacggccg tgcggtcgcg gtcaaggtcc agtacccggg ggtcgaccgg
     2281 gccgtcgccg ctgatctgga gagctcggac ctgctcttcc aggtcctggg gatgctgttc
     2341 ccggggctcg accccgctcc gatcgtcacc gagctgcgcg agcggctggt cgaggagctc
     2401 gactaccgca tcgaggccga caaccagagg ctgttcaccg accgctaccg cgaccacccg
     2461 ttcatccacg ttcccgccgt gttcgacgac ctgtccaccg cccgggtgct caccaccgag
     2521 ctggtcgagg gggcgccgtt ctccgaggtg gtcgactggc ccgacgacga gcgccagctc
     2581 gccgcggagt gcctgtaccg gttcgcgttc ggcgggatct accagctgca cgcgttcaac
     2641 ggcgacccgc acccgggcaa ctacatcttc catcccggcg gccgcatcac cttcctggac
     2701 ttcgggttgt gcaagcggtt cagcgaacag gaggtggcgg acttcgagga catgatccag
     2761 gcgatggtgc tcgagcgcga cgtcgcccga ttccgccggg tcatcgagcg tctcggcatc
     2821 ctgtcgccgg agctggtggt gtccgacgac gagctggtcg cctacttcgg gcacttctac
     2881 gagttcgtga tggaacccaa ggagatggag atcaccccgg agtggtcctc ggactcggtg
     2941 cggcgcttct tcgacctgac cggcgagcac accgacatca tgaaggcggc caacctgccg
     3001 ccgtcgatgg tgatcgtcca gcgcatcaac atggggctgt tcgccctgtt cggcgacctg
     3061 cacgcccggg ccaactggcg ggagatcgcc gaggagatct ggccgttcac cgacggggcg
     3121 ccgtcgacgc cgatggggga gcggatcgcc gagtgggagc agacccgcac ggtcgcctga
     3181 caggccatcc gacccggcga cggcgtaccg gctctactcg acgatgcgga tcgggttgcc
     3241 gaggatcgcg gcggaccacg tggtggcgtc ggtgacggtc gcgctcgacg ggtacgccgc
     3301 cttgatcttg atccagcacc cggtcggcga gtcctcgtcg cagtcgtagg tggtggggat
     3361 cggaacgtcg atcgtcagca gccgaccgtt gtacgagctg ctcgacacgt tgctgagcgt
     3421 gcacgtggat gcgttgacgc tgaggccggc tccgtcgtcg cgggagatgg cgcagccgct
     3481 gaacgtggtc agaccgttgt aggcctcggc cggcggcagc acctggaggc tgccgggctg
     3541 ggagctctca cccatgtcga acaggttgat gcgcagcgtg cgtccggcgt cgtaggggag
     3601 caccttggcc aggtagaagc gggtgtcagc cccgttggcg ttggcgtaga tcggaagtcg
     3661 acccagagcg gacaccgtga cgttgctgcc gttcaccgcg ttgaccccgg cggtgccgaa
     3721 cccggtccgg aaggccatgt ggttgtggcc ggccgtggac accgacgggt tgtaggtcgt
     3781 cgggctcgcg gcggtgacgt tggtccggac ctggacgatg tacttgccgg gttgcaccga
     3841 cccgttcgga atcttgcaca gggtcgccca ccggcggaac gtctctccgt aggtcagcac
     3901 cccgtcgttg gggttgatgg tcgagcccat gagggcgtcg tagatcgccc ggcccgagtt
     3961 cgacgtcttg gtcgggtcga agccgttgat cgtgaccggc gggcactggg tgaccggcgg
     4021 gttgtcggtg tcgttccagg gtgtgctgtc gggctgcagg aaccggaacg tggtgttgac
     4081 ctggccgtag gaggagttgc cgtggccgtc accggtgcag tacttgttgg ccggggccgt
     4141 ggtcgccgag ctgtagcggg ccgcggcgtc ggggtacttc gtggtcagag ccgtgtaccc
     4201 gctgccgccg ttgatctggc tcgaggtcgg catgtactgc gagcagctga ggttgttcga
     4261 cgtccacaca ccgtcgtaca cctcgaacat caggtcgccg gtgcccttcg aggtgacgtc
     4321 catggcgtag aggtaaccgg cggtctcgta ctggtcgttg gtggtgccgg tgcagccgta
     4381 ctcccgcggt gagccgccgc acaccttgga ctggtactgg tcgccctgct gcttggcggt
     4441 ctgcgggccc gagatgttca cccacagctg cgtgccgggg tcgaccgcgt cggggtcgtt
     4501 gccgagcttg ttctgcgggg atcccatcgg gaccggcgcc acgtactccg ccaccgcctc
     4561 gcgggtcatg cgcacgtcgt ccatgccgat cagccccagg aagaacgtcg ggacgtcggt
     4621 ggtcaccttg acccggagcc ggttgggatt gggctcctgg gtgaccgcga cggtggtgtt
     4681 ccgcggcccg ccgaccgggt agccgttctt ggccacggtg ccggtcgcga cagtcgtcgc
     4741 ctggggcagg tccgccggca ggaagatcac cccggagtgc gcacccgcgt ccgcggcgcg
     4801 ctggatccgc tcggcctgga agtaccagtt ggacacgtcg accgcgaaac cggcgaagag
     4861 caccatcgtc agcagggtca gggcgaacca caccatggcg atgccgcgct cacgccggtt
     4921 ccgctcccgc cacggcgtga tccgacggcg gatcggaccc gagcgctcgt cccgccgcgt
     4981 caacactggt cacccggcag cggctcgagg cgcatcaccg tgcgcccctc gacgaagttg
     5041 tactgaccga tcagcttggt gaggtagctg tggcggatca gcacccacac cccgaccgaa
     5101 gcggtgctgg ccggcaggca cacgtcgttg gtcgacaggt tccaccccgg tccgccgctg
     5161 ggcgtgaact tcttggtcga cgtgttccag gtgtagcgca tgcagtcggc ggtgcagccg
     5221 ccgctcatcg acgccgtcga ggacgggtac ccaccggtcg gtgcccccga cgagttcgcc
     5281 ttgtagatga ccatgccgat cgggaccgcg ctggtgagag cggtcagatc gcccgacacc
     5341 tgatcggcca ccagatccgc cggtccctgc acgctgccgg tcaccgcggc cttgttgggt
     5401 ccgaacgtgg ccaccgccag ccgggcgccg gttcggctgg ctcccgtcgc ggtcgcaccg
     5461 gtggcgtaca ccaacccgaa ctcgatgatg cccagcagga acaccatcag caccggcagg
     5521 atgatcgccg cctcgatcag ggccgcgccg cggtcggagc gggtcgtgcg gtggtggtac
     5581 acacggccga gtcgggatcc ccgccacccg tgtgctcccc gcccgctgtc agcggtccgg
     5641 ttcgtcatca caggtaccgt gtcggcagcc gaggcctgcg acttgagcct cccgacccga
     5701 cgatcgctgg gtcgaaccgc ccagaggggg agaccgacga accatgcccg tcgacgccga
     5761 actgcaaccc gtgctcgacc tcctcgccgc gatggagcag ccgccaccca tggaggccgg
     5821 tcccgacgcc gcccgtgagg gcttcgccct gttgtgcgcg gcgttcggcc ccggcgccca
     5881 ggacgtgacc gcgaccgaca tcaccctcgc cggtgccgac ggtccgatcc cgggtcggat
     5941 ctaccggccg gccggagcgg cgccgagcac cacgggagcc gccggcctgg tcttcctgca
     6001 cggcggcggg ttcgtcatcg ggtccctcga cacccacgac gccctgtgtc gcgacctggc
     6061 ggccggagcc gacatcaccg tcgtgtcggt cgactaccgg ttggcccccg aacatccggc
     6121 tcccgccgcc gccctggacg ccgcctccgc cctcgacgac gtggtgcgcc gtgccccgga
     6181 gctcggactg gacccgtcgc ggctggccgt cggaggcgat tcggcgggcg ggaacctggc
     6241 cgcggtcacc gcagtggcct ggcgtgatcg ccagcgcgac gatccgggtc tggcgccgtt
     6301 gcgcctgcag gtgctgatct accccgcggt cgagctcgcg gatgaggatc gcttcccctc
     6361 gatgaaggag aacgccgagg ggtacctgct cacggccgag acgatggcct tcttccgcca
     6421 ccactacctg acgggggcaa ccgtcgatgc cgcggacccc atggtcagcc ccctccgcac
     6481 cgcggacctg gcgggggtcg cccccgcgct ggtcctcacc gccgagtacg acccgctccg
     6541 ggacgagggc gaggcgtacg ccgccgccct cggcgccgcc ggggtggagg tcgacctcga
     6601 acggttcgac ggggccatcc acgccttcgt gcagatgggc agcaccggga tcggccgaca
     6661 ggccgtcgca cggatctgcg ctgctctgcg ggacgctgtg tcctgagggc cgatctggcg
     6721 accctgggcc cggacgctcg gtggagatag cgtgacgccc atgacatcga ccatggggca
     6781 ggtcagggtg gacgagcgcg gtggggacgc cgagaccgcg tcgggtccgg agggcaacgg
     6841 ccgcgccgtg ggcaccgcga gcgatggccg aacgcccggc gccgcgacga tcgagacgtt
     6901 cgacccacgc accggcacgt tgctggccgt cgtgccggac cagaccgcgg acgaggtccg
     6961 cgccgcggtc gctcgggccc gtgccgcagg cacggcatgg tcggccctca ccgcccgcga
     7021 acggtcccgg cacatgctgg cggtccggga ccggctgctc gaccgcgccg aggagctggt
     7081 acgggtcatc tgcgccgaga ccggcaagca ggccgccgaa gcggtgacca ccgagctgat
     7141 ggcggtctgc gagaccatcg actggtacgc ccgcaacagc gaacgcatcc tgcgggccca
     7201 gcccgtcggc tcggggacca tggcgcacaa gtcggcctgg aagcgctacg aacccctcgg
     7261 cgtcatcggc gtgatcagcc cgtggaacta ccccttcacc ctgtcgatga ccccggtcat
     7321 caccgctctg ttcgccggca acacggtggt gctcaagccg tccgaggtca ccccgaccgt
     7381 cgggctcgcg atcggcgccc tgttcgagga cgacacgtgg ggcgacgtgg tccaggtcgt
     7441 caccggtggc ggagcgaccg gcgaggcgct ggtgcgctcc ggagtcgaca agatcgtctt
     7501 caccggctcg gtccgcaccg gtcgacgggt gatggccgcc gcggccgaca ccctcacccc
     7561 cgtcctgctc gagctcggtg ggaaggaccc gatggtggtc tgcgaggacg cggacgtggt
     7621 ccgcgccgcc aagggggcgg tgtggggctc catgcagaac tcgggtcaga cctgcatgtc
     7681 ggtcgagcgc gtgtacgtcc acgaatcggt ctatgaccgc ttcgtctccc tggtcctgtc
     7741 cgaggtggcg gccatccgcc aggacgacca cggcggcgac atcggctcga tgaccttcga
     7801 accgcagctg cagatcatcg aggcgcacct ggccgacgcc ctcgacaagg gcgcgaccat
     7861 cctcgccggc ggatcccgcc tcgaccgcga cggcctgtgg ttccccccga cggtggtggt
     7921 cgacgtcgac cacgacatgg acctgatgac cgaggagacc ttcggtccgg tcctgccgat
     7981 catgaaggtc tcgagcgacg aggaggccgt ggcgctggcc aacgactcca gctacgggct
     8041 caacagctcg gtgtggaccg aggactccga ccggggggtc cacatcgcca accgactcca
     8101 ggccggcaac gtctgcatca acgactgcat cgtgtcctac gcggtgaccg gcctgccctt
     8161 cgggggagtg aagagctcgg gcatcggacg ggtgcacggc gctgagggcc tgcgcgagat
     8221 gtcgatcgtg aagtcgatcc tggccccccg ggtcaggacc ccccgggagc tgtggtggtt
     8281 cccggtgccc cggaacctgg accggatcgg catcgccacg ttgcgcgccc gtttcggcgg
     8341 caacctgcgc accaagttgg ggcgacgtcc aggggtctga ccgcgttcag gcctgggtgt
     8401 cgcgcagggt ccgcagaacc aggcgggtga cggcctccgc aggtggggag tcgcccccgc
     8461 tcagcgcctc gacggtgcag aacacctcgg tggtgccgtc ggcgcgcccc tggaggctga
     8521 agagcaccga gctggtcgga gcttcgacga actcgacgat cacctcgagc agccctgccg
     8581 tgcgatcgag gtagctgatc tctccgaggt cgtgcgccgc gtcgatgagg agcatcaggg
     8641 cctcctccgc gggggccgac accgagatcg agtcgttgcc gacgagtcgc acgccgatgc
     8701 gtggaacctc ccgtccgggt ccgggcacat cgccacgacg ggcgctggcc gcgtcgcctg
     8761 aaggcgccgg gccccgctcg ggcgtgctgc caccggatcc gccgggcgca ggccggtcct
     8821 tcgatgatcg ggcacccacc gacgtcgtcg actcacctcg gagcgcagcc tcgctgagga
     8881 cccggtaggc caaggtcagg cgggtggtcc gttcggcggc atcggcgccg gcgttgacgt
     8941 cgggatgagc gacgcggacc agccgccgga acgacttccg gaggaccttc gcgtcgatcg
     9001 gtgcgtcggg gtccagtccg agcacccgtc gtgcctcgtc gacccgcacg ccnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccggtcga tcccttgaca gccgtcgtac
     9181 catgagatct ccccgagcgg cggcggactc ttcctcacca gtgcacccat ccacgcggaa
     9241 cgcgggttcc gacacccttg gcatgtgagc gcaggtcccg acggcgaacg accatcccac
     9301 cgagatcacc cggccaaccc atgcgggcgc cggatccggc gtagacgcga cagaaaggcg
     9361 atcgcccgtg gcgaagcaac gagcagagcg cgacgaggaa gacctcgttc gactgtacct
     9421 gagcgagatc ggccagtacc ccctgctgac caaggacgac gagtcccgcc tggcccaggc
     9481 catggagcac ggcaaggagg ccgccaccga gctggaggcc aacgagcgca gcctgtcgcc
     9541 ggcccgcaag cgcgagctgc ggaagctggt caaggccggc gaggaggccg agcgctcgtt
     9601 cgtccagtcg aacctccgac tcgtcgtgtc gatcgccaag aagtaccagg cgtccggcat
     9661 gccgctactc gacctcatcc aggagggcaa cctcggcctg atgcacgcgg tcgagaagtt
     9721 cgactggcgc aaggggttca agttctccac ctacgccacc tggtggatcc gccaggccct
     9781 cacccgtggc atcgccaaca ccaaccggac catccgtctc ccggtccacg cgggcgacaa
     9841 cctggcgcgg gtgcagaagg cccgcgtccg cctcgagacc gcctacggac gtcggcccac
     9901 cgtcgccgag ctcgctgccg acctcgacat gcccgaggac aaggtcaccg agatcctccg
     9961 cttcgccgcc gacacgctgt cgctgtccac gccgctgcgc gaggacggcg acgccgagct
    10021 cggcgacatc gtcgaggacc cgaccgcggc gtcgccgttc gaggccgcgg ccgagtcgct
    10081 cctcccggcg cagatcagcc gtctgatgat gccgctcgac gaccgggagc gcgagatcct
    10141 ctcgctgcgc ttcggtcttg accggggtga gccccggacc ctggaggagg tcggcgagca
    10201 cttcgacctc acccgggagc gcatccgtca gatcgaggcc cgggccatgt ccaagctgcg
    10261 tcaccccagc tccgacaccg gcgcccgcga cctgctcaac gcctgatccc cttcgtcacg
    10321 accggggccg gatccgacag ggtccggccc tcgtcgcgcc ctgggacgga accccgcccg
    10381 gatttctggg gtcggaaaag cgacaagggt tgtgagtgag gacccctata tcgcctggag
    10441 ggcggtgggg agggaggtga tgtcggcgac ggccatcagc tcacgcgccg aatgcatcga
    10501 cagcatcggc gcaccgacgt ccacggtgtc gaccgccagg cgcgacgcgg tgatcggccc
    10561 gatcgtcgac ccgcacggca ggtcgccgcg gtgcgagtac tcctggaccg gcaccccgac
    10621 ccgatcgcac gcagcccgga acgccgccgc cccggtcgac tcggtcgcgt agcgggcgtt
    10681 gacgttgtgc ttgatcaccg gcccgccgcc gggtcggatc cagtgcccgg gttcatgacg
    10741 ctcggggtag ttggggtgcg tggcgtgcgc catgtccacc gacaccagca gcgacccggt
    10801 cagggagcgg agcaggtcgg tccgggttcc gccgagcgcg gccacccggc gttcgagcac
    10861 ctgctcgacc cacgccccgt cggcgccggt cgcggtctgc gagccgacct cctcgtggtc
    10921 gttgagcacc accaccgtcg gcagctcagg cgggtccatc accgacgccg cgtgccggac
    10981 cgcggttagt gccgcccagc agccggccag gtcgtccagg cggggggcgg ccagcatgtc
    11041 gcgctcggcg ccgatcaccg ccgagggctg cacgtcgtag gccagggcct cccatgcgac
    11101 cacggcgctc gcctcgacgc cgaccagcgc ggcgagccac gcccggaagt cgccgtcgga
    11161 cgcgtcgccc aggccccaca ccggagtcag gtgctgctgg gcgttcagga ccagaccggt
    11221 ggtgctcacg tcgcggtcca ggtggatcgc cagctgcggc accctcatga ccgcaccatc
    11281 ggtgcggaac cggcgcagct cgacggcgtc gtcgctgcgc acggcgagcc ggcccgccag
    11341 gcccaggtcc cggtccagcc acgagttgcg cagcgctccg ccgtagagct cgacgccgag
    11401 ctggcgccac ccggcggtcg aacggtcggg tcgggggcgg acccgcaggc ccggtgagtc
    11461 ggtgtgggcg ccgatcatcc gcagcggggc ggccggatca ctccgtcgtc ccggacacca
    11521 ggccaccagg gcgccgccgc ggacgacgta ggcgggtccc gccgcctcgg gtccccagtc
    11581 ctcgatcggg tcgagggcgg tgaagccggc gtggtcgagg agggccgctg cggtcgcgac
    11641 ggcgtggtag ggggtggggg aggcgtcgac gaaaccgatg aggtcgctga ggtcctccgc
    11701 gaccgtcgtg ggatcctggg tcatcgtcgc ctcctgtcgg tcagctcgcc tgcggcgtga
    11761 gccctgcgca ctgctggcgt tgggtcatgg tcgcctgcgc cgcgtcggcc gccgtgccgg
    11821 tcaacgggtg cgggatgctg ctgcggatca ccgccggggt ccaggtgtag ccgtcgatgc
    11881 gtcgccccgt ggcggtcacc atcagcacgc cggtggccgc tccctcgggt gagttggcct
    11941 ggaacgcgaa gttgcccagc gagtacgcca cgaactgctg accgtggtag ccgaccccct
    12001 ggagccggtg cgggtgcgag ccgaccacga tgtctgctcc ggccccggtc agcagctcga
    12061 cgagctcctt ctggcgggcg ttggggcaca gctgggtctc ggttccgtag tgcaggaaca
    12121 cgaccagggt gtcgacct
//
LOCUS       DAOQOD010000095        11986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1894_length_11986_cov_12.727852, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000095 DAOQOD010000000
VERSION     DAOQOD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_11986_cov_12.727852"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..979
                     /locus_tag="PLZ72_10145"
     CDS             89..979
                     /locus_tag="PLZ72_10145"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNA36699.1"
                     /translation="MGVFSRSFALFKASWGVLRHDKELLALPVISAVTSFLCVAPFVG
                     LIAVTALDSATRGVATSGSLDGLSIGPLGYVLMFVAYLIGAYVTIFFQSALVLAANER
                     LSGGEPTLGWAISAAASNAGRILPWAIISATVSIVLQAVQERAGFIGRIVVGLVGMAW
                     TLVTFLVLPILVIEQVGVKDALSRSASAFKRTWGENVVGNGGIGLVMMLVGLLAFVLT
                     APVAFVGASTGNTPLLVLGVILVVVAMVAVSVLGAALSGVFRTALYRYAVLGEEPTGF
                     THDQIASAFRPKRTRGASAI"
     gene            1085..1726
                     /locus_tag="PLZ72_10150"
     CDS             1085..1726
                     /locus_tag="PLZ72_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNA36700.1"
                     /translation="MTITDTPTPTDDGTLVLSISDAALATVLGIRDQEDDAEALGLRV
                     EITGSKGAEFTYDLGFEELTSADADTLVEQVAGGLKVMIPAATVENLRGSVLDLSRAG
                     AQGGLVIRNPNRPDPLAGIDLELTGDVAERVTQLLDQAINPALGSHGGYATLVGVDGE
                     VVYVTMGGGCQGCAASAATLREGIRRSILDAIPEVTDVVDATDHTAGENPFYT"
     gene            complement(1779..3662)
                     /gene="dxs"
                     /locus_tag="PLZ72_10155"
     CDS             complement(1779..3662)
                     /gene="dxs"
                     /locus_tag="PLZ72_10155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNA36701.1"
                     /translation="MEDHATTILDTISSPDDLAGLDESQLTQLAAEIREFIVDTVNAR
                     GSGHLGSNLGVVELTIALHRVFHSPHDIIVWDTGHQAYVHKLLTGRQADFDRLREEGG
                     LSGYPSRTESDHDWVENSHASTAVSYAHGLSTAMHQRGETDREVVAVIGDGALTGGMA
                     YEGLNNLGHSGRKAIIVLNDNGRSYAPTASRLGESLSRFRASSTYLRNRERLERLIQD
                     VPLVGNYLERGVGGAKAALREMFEPPVFFEQLGVHYLGPYDGHDVTAVETALRAARDL
                     DEPVVVHVLTRKGLGYGPAEADQVKHMHDVGGVKDGSYTAAFSDAMMVEAAARPELVA
                     ITAAMPDSTGLLPVAARFPDRVLDVGIAEQHAVTSAAGMAMGGLRPVVALYSTFLTRA
                     IDQVNLDVGLHSQPVVFALDRAGITGDDGPSHHGVLDMVLLSKVPDMTIFAPSSHAEV
                     ARMLHDALDLCTDGPAAIRYPKTMPPDPADPELGVCGEGLAARRLRTGSDVCLIGVGK
                     MLAAARDAADRLAAEGHDVTLWDPRVVKPLDPEMLADAASHRLVITVEDGLRDGGIGA
                     AIADSLSKLAPVDGPLVRVLGVPSVFLAQGKPDAILSRLGLDADGIADEALAWIRSAD
                     RTV"
     gene            complement(3676..4785)
                     /locus_tag="PLZ72_10160"
     CDS             complement(3676..4785)
                     /locus_tag="PLZ72_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNA36702.1"
                     /translation="MEHTDPADDRTAALEEIERSEIGDPDRPPEVPRNHWSRIVARVA
                     VSLGFLGLLFWRLPEVQLDELFPSPTPATWVWIAAAVGVHLVAYLLQNLRWSAVSDTL
                     GIPLGFRRLLGHLLAGEFVSNALPTSFGGDVVRVMRQGHDVGDYAESFAATSLERLTG
                     WLVLPTLSAVGLLLGTGYLSLGAASTVAVLTDVLTFVALLGLLWVAGHPRGAGRLVGR
                     SGWRRYLGAVHLGIVAFHGRRAQAVRVLAAGVGFQFLQCVSVWCAARALRIDEVTLVA
                     SLAFFPPTAVLQNFPLALGGLGVREAAFVLFFGALGVSHSAAIGLGLLVYVVFVLTSL
                     AGAPSFARGGLGGRPRSAGRGPRPAGQPDVAADPE"
     gene            4875..5825
                     /locus_tag="PLZ72_10165"
     CDS             4875..5825
                     /locus_tag="PLZ72_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36703.1"
                     /translation="MTIDERDDRRPSPALGFMTDLASVADIRPVAVPLTPGDPAARGG
                     ADTIDLTVAEAGVGGRPLLLVHGFTGAKEDFRDVVAPLAAAGHWVVAPDLRGHGAGPH
                     PPGEDSYGLDRFAADLSGLVDVLGWDRFDLLGHSMGGMIAQKVALAHGSRIDRLVLMD
                     TATGGVDGSEHGADPDNAALMRIGVELCRSEGIAAIAAVLQMGDQPLESPAHQRLAEQ
                     PGWTEWESGKFLACSGDMWCAMVEEFLSTPDRMDLLASLPMPTLVVVGEQDRAFRAVS
                     ARMAEVISDARLSVIPDAGHSPQFENPSAWYAAVAGFLAR"
     assembly_gap    5842..5941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5942..6492)
                     /locus_tag="PLZ72_10170"
     CDS             complement(<5942..6492)
                     /locus_tag="PLZ72_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HNA36704.1"
                     /translation="MEPTALAQAQDIAAGLLAQVGADQLQAPTPCAEWNVGQLIDHMV
                     GTQDWARAAIDGVELTDTGDGASADDFRARYADAAAACLAAFQADGALERTVDPGFGE
                     MPAAALLGIATTDTFTHAWDLATALDLDNDLDPELAELLLDNAHRAIPPSFRTEDGSI
                     FRAEQDAPAGANAATRLAAYLGRT"
     gene            complement(6573..6950)
                     /locus_tag="PLZ72_10175"
     CDS             complement(6573..6950)
                     /locus_tag="PLZ72_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNA36705.1"
                     /translation="MSIRTGLSAAIDLTVSDADTAIAMRSGEVPVLATPRILALVEEA
                     TVRALDGHLDEGSSTVGMRVQLEHISPTAIGGVVRAEANLEKVEGRRLLFHVSARDER
                     GLVAAGKVTRVVVDVDRFMEKTH"
     gene            7123..8553
                     /locus_tag="PLZ72_10180"
     CDS             7123..8553
                     /locus_tag="PLZ72_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNA36706.1"
                     /translation="MPDAPTDRSSEPALLDQAVVERVLGAGLARGADFAEVFVEDRRS
                     SSALLDDGRVEELTSGRDRGAGIRVVVGDSTGFAHTSDLSEAGLIAAAEAAAAASRGA
                     GGSTRVVDLDRVDAPPPNAVAILPGDVPKARKVELLLTADGAARAAGGAITQVSARYA
                     DGRRRILVANSDGVLASDDQVRTIFSVSCVASGDTGMQTGRESVGHTVGFELFDGVEV
                     ADLAERAARRALTKLAARPAPSGEMTVVIGPGGGGVMFHEACGHGLEADLVGKGASVF
                     AGRLGEQVASPLVTLVDDGTMGGEWGCFAVDDEGRPAQRNVLIQDGVLVDYMWDGLRA
                     RKEGRASSGNGRRQSYQHLPMVRMTNTYLLGGTSSPDDIVADTPSGVYVAHLGGGQVN
                     TATGDFVFGMTEAYLIEDGRITEPLREGNLIGNGPAVLAAIDAVGDDFAMGAPGTCGK
                     DGQGVPVGDGVPTLRAHGLTIGGTAA"
     gene            complement(8763..9563)
                     /locus_tag="PLZ72_10185"
     CDS             complement(8763..9563)
                     /locus_tag="PLZ72_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNA36707.1"
                     /translation="MTGALSVVVASQDLVRDGTLLIALPIAVLAGVVSFLSPCVLPLV
                     PAYLSYVTGVSVAELSEGGSSTDVHTMRRVVIGTLGFVAGTAVVFVSFGAAFGSFGDA
                     LQRHAVGLTQILGVVTIFLGLLLAGAFSRSDLLNRDLRIHRIPGQGLLAAPIIGLTFA
                     LGWTPCIGPTLGVVLGLSASSDQASAWRGAVLSLAYCLGLGIPLLISGLAFNRAMTVF
                     GVIKNHYRALMVGSGAMLVVLGVLQVTGVWTEWMNRLQTSFGGNSLPL"
     gene            complement(9560..10081)
                     /locus_tag="PLZ72_10190"
     CDS             complement(9560..10081)
                     /locus_tag="PLZ72_10190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA36708.1"
                     /translation="MTAARAHRATATVGLLIAAVAISVLGTACGGGGSDDAYVAEDLR
                     SGQDVSVQDLRGEPVVLVSWATWCKECDEVLSGLQKFSSSEDAKGLEVVAVNLDAASV
                     DDEIDAKIERHGLSTQLWRDRRNEFKRSFKALGVPTTVLLDRDGEVAATFPGAVDFDS
                     AEVRSALEEIRSS"
     gene            complement(10078..10428)
                     /locus_tag="PLZ72_10195"
     CDS             complement(10078..10428)
                     /locus_tag="PLZ72_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36709.1"
                     /translation="MIAATISVLALLTAGVAWLVVSRDSGPEPRTVHLVVEAGTQDRV
                     NAGEKVVMMPSYMELRVGDTLFIENKDAVPQWVGPYLVGPGNSFSFTYGSPGRFAGFC
                     PLADGQTYEIVVKE"
     gene            complement(10556..11179)
                     /locus_tag="PLZ72_10200"
     CDS             complement(10556..11179)
                     /locus_tag="PLZ72_10200"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNA36710.1"
                     /translation="MHTDRIRRSVAAPMALALTAGVFLVGCGDDGKESSSSTTRPEAS
                     TSQPADDAAISTSGVWARTSPAVASAGAVYLKITNDSDSDDALVAASVDPSVAKKTEL
                     HETKAAEGGMSSTTAPEQGMGQGQSGDAHGSSTTAMGGGGMMTMAEVDKIPVAAGTTV
                     ALEPGGYHIMLMELAKPLVVGEKVKVTLTFEKAGDVVVEAEVRDNAP"
     gene            complement(11181..11840)
                     /locus_tag="PLZ72_10205"
     CDS             complement(11181..11840)
                     /locus_tag="PLZ72_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNA36711.1"
                     /translation="MSDNFRQRRRVRTGRIVALLVALTLVGAACGSDTTDDDASGSGD
                     LLGAVRNPPLAVGDVTLTDTSGATPRSVTMTPPKGDLYVVYFGYASCPDICPTTLSDI
                     QVALGDLDEADAKRVTVGMVTVDPERDTPELLSEYLSHFFDRSMALRAETPEALTAAT
                     EAFGVRYEIAPHPPGATDYEVSHTAVTYVIDDTGKVAVEWPFGFDSEHMTKDLNTLLE
                     G"
BASE COUNT         1661 a         4064 c         4450 g         1711 t
ORIGIN      
        1 gctttgtccc gcacaggcgc ggtgatcacc gcgcctgtgc gggacaaagc gggcggaaat
       61 cggggggccg ccgggcagaa tgtcgaccat gggtgtcttc tccaggtcgt tcgcactgtt
      121 caaggcctcg tggggggtgc tccgccacga caaggagctg ctcgccctgc ccgtcatctc
      181 ggcggtgacg tcgttcctct gcgtcgcccc gttcgtgggc ctcatcgccg tcacagcgct
      241 cgactcggcc acccgcgggg tggcgacgag cgggtcgctc gacggcctga gcatcggacc
      301 gctcggctac gtcctcatgt tcgtggccta cctgatcggc gcgtacgtga cgatcttctt
      361 ccagtccgcg ctggtgctcg cggccaacga acggctcagc ggcggcgagc ccaccctcgg
      421 ctgggcgatc tcggccgcgg cgtccaacgc cggtcgcatc ctgccgtggg cgatcatcag
      481 cgcgacggtg tcgatcgtcc tgcaggcggt ccaggaacgg gccggcttca tcgggcgcat
      541 cgtcgtcggc ctggtgggca tggcctggac cctggtgacg ttcctggtgc tgccgatcct
      601 ggtgatcgaa caggtgggcg tgaaggacgc tctgagccgg tcggcctcgg cgttcaagcg
      661 gacctggggc gagaacgtgg tcggcaacgg cgggatcggc ctggtgatga tgctcgtggg
      721 cctgctcgcc ttcgtcctga ccgcaccggt ggcgttcgtc ggcgcgagca ccggcaacac
      781 gccgctgctg gtgctgggcg tgatcctggt ggtcgtggcg atggtggcgg tgtcggtcct
      841 cggtgccgcc ctgagcgggg tgttccgcac cgcgctgtac cgctacgccg tcctcggcga
      901 ggagccgacg ggattcaccc acgaccagat cgcctcggcg ttccggccca agcgcacccg
      961 cggcgcctca gccatctgac ggcgtaccgg gcgtgtcccg gagccgccgc ggctgtcgag
     1021 gctcgcattt ctcctactac gatgggagaa atccgggcgg tggtcgtccg ggttggagcg
     1081 cccgatgacc atcaccgaca ccccgacccc caccgacgac gggaccctcg tcctgtccat
     1141 ctccgacgcc gcgctcgcca ccgtgctcgg catccgcgac caggaggacg acgccgaggc
     1201 gttggggctg cgggtcgaga tcaccggctc caagggagcc gagttcacct acgacctggg
     1261 cttcgaggag ctgacgtcgg ccgacgccga caccctcgtc gagcaggtag ccggcgggct
     1321 gaaggtgatg atcccggcgg cgacggtcga gaacctgcgc gggtcggtgc tcgacctctc
     1381 gcgggccggt gcccagggcg ggctggtgat ccgcaacccg aaccgtcccg acccgctggc
     1441 cggcatcgac ctggagctca ccggcgacgt cgccgagcgc gtcacccagc tgctcgacca
     1501 ggcgatcaac cccgcgctcg gatcccacgg cgggtacgcc accctcgtcg gcgtcgacgg
     1561 cgaggtcgtc tacgtcacca tgggcggcgg atgccagggg tgcgcggcga gcgcggccac
     1621 gctgcgggag gggatccggc gatcgatcct cgacgcgatc cccgaggtga ccgacgtggt
     1681 cgacgccacc gaccacaccg cgggcgagaa ccccttctac acctgagacg ctgcgccgtc
     1741 gtcggggtcg gcgccgacga catcggccgg tgagtcgctc agaccgtgcg gtcggcgctg
     1801 cggatccagg ccagggcctc gtccgcgatc ccgtcggcgt cgagccccag gcgggacagg
     1861 atggcgtcgg gcttgccctg ggccaggaac accgacggca cgccgagcac acggaccagc
     1921 ggcccgtcga ccggggccag cttggacagc gagtcggcga tcgcagcgcc gatgccaccg
     1981 tcgcgcaggc cgtcctcgac ggtgatcacc aaccggtgag acgccgcgtc ggcgagcatc
     2041 tccgggtcga gcggcttgac cacccggggg tcccacaacg tgacgtcgtg accctcggcc
     2101 gccagtcggt ccgccgcgtc gcgtgccgcg gccagcatct tgccgacccc gatcaggcag
     2161 acgtcggacc cggtgcggag cctgcgcgcc gcgaggccct cgccgcagac gccgagctcc
     2221 gggtcggccg ggtccggagg catggtcttg gggtagcgga tggcggccgg accgtcggtg
     2281 cagaggtcca gcgcgtcgtg cagcatccgg gcgacctcgg cgtggctgga cggggcgaag
     2341 atcgtcatgt cggggacctt cgacagcagg accatgtcga gcacgccgtg gtggctggga
     2401 ccgtcgtcgc cggtgatccc ggcgcggtcc agggcgaaga ccaccggctg ggagtggagg
     2461 ccgacgtcca ggttgacctg gtcgatcgcc cgggtcagga acgtggagta gagggccacc
     2521 accgggcgga gcccgcccat ggccatgccc gccgccgagg tcaccgcgtg ctgctcggcg
     2581 atgccgacgt cgagcacccg gtcggggaag cgggcggcca ccggcagcaa gcctgtcgag
     2641 tcgggcatgg cggcggtgat cgccaccagc tcgggtcggg cggcggcctc gaccatcatg
     2701 gcgtcggaga acgcggcggt gtacgagccg tccttgacgc cgccgacgtc gtgcatgtgc
     2761 ttgacctggt cggcctcggc gggtccgtag cccagtccct tgcgggtcag gacgtggacc
     2821 acgaccggtt cgtcgaggtc gcgggcggcg cgaagagcgg tctcgaccgc ggtcacgtcg
     2881 tggccgtcgt aggggccgag gtagtgcacg ccgagctgct cgaagaacac cggcggctcg
     2941 aacatctccc ggagggcggc cttggccccg ccgaccccgc gctccaggta gttgccgacc
     3001 agcggcacgt cctggatgag gcgctcgagt cgctcccggt tgcgcaggta ggtggagctg
     3061 gcccggaacc tcgacaggct ctcgcccagc cgggacgcgg tgggtgcgta cgagcgaccg
     3121 ttgtcgttga ggacgatgat cgccttgcgg ccggagtggc ccaggttgtt caggccctcg
     3181 taggccatcc caccggtgag ggcgccgtcg ccgatgaccg cgacgacctc ccgatcggtc
     3241 tcgccgcgct ggtgcatcgc ggtggacagg ccatgggcgt aggacaccgc ggtggaggcg
     3301 tggctgttct ccacccagtc gtgatccgac tcggtccggc tcgggtagcc cgacaggccg
     3361 ccctcttcac gcaaccggtc gaagtccgcc tgccgcccgg tgagcagctt gtgcacgtag
     3421 gcctgatggc cggtgtccca cacgatgatg tcgtgcgggc tgtggaagac gcggtggagc
     3481 gcgatggtca gctcgaccac acccaggttg gatcccaggt gtccgcttcc ccgggcgttc
     3541 accgtgtcga cgatgaactc gcggatctcg gcggcgagct gggtgagctg tgactcgtcg
     3601 agcccggcga ggtcgtccgg ggagctgatc gtgtccagga tggtcgtggc gtggtcctcc
     3661 atcggtgctc ccacgctact ccgggtcggc ggcgacgtca ggctgccccg caggccgcgg
     3721 tccccgtccg gcgctccgcg gtcgaccccc gaggccgccg cgggcgaagc tcggtgcgcc
     3781 ggccaggctg gtgagcacga acaccacgta caccagcagg cccaggccga tcgcggcgga
     3841 gtggctgacc ccgagcgctc cgaagaagag gacgaacgcg gcctcgcgga ccccgaggcc
     3901 gccgagggcg agggggaagt tctgcagcac cgcggtcgga gggaagaacg ccagcgaggc
     3961 gacgagggtg acctcgtcga tgcgcagcgc ccgcgccgcg caccacacgc tgacgcactg
     4021 gaggaactgg aatccgacgc cggccgcgag cacccgtacg gcctgggccc gtcggccgtg
     4081 gaacgcgacg atgcccaggt ggacggcgcc caggtagcgc ctccagccgg aacgtcccac
     4141 cagtcgaccc gccccccggg ggtgaccggc gacccacagc aggccgagca gcgccacgaa
     4201 ggtcagcacg tcggtcagga ccgccacggt gctggccgcg ccgagcgaca ggtatcccgt
     4261 gccgagcagg aggcccaccg cggacagcgt cgggaggacg agccagccgg tgagccgctc
     4321 caggctcgtc gccgcgaacg actccgcgta gtcgccgacg tcatggccct gtcgcatgac
     4381 tcggacgacg tcgccgccga aggaggtggg cagggcgttg gacacgaact cgccggcgag
     4441 caggtggccg agcagtcgac ggaagcccag cgggatcccg agcgtgtcgg acaccgcgga
     4501 ccaccggagg ttctggagca ggtacgccac caggtgcaca cccaccgcgg cggcgatcca
     4561 cacccaggtg gcgggcgtcg ggctcgggaa cagctcgtcg agctgcacct cgggcagtcg
     4621 ccagaacagc agacccagga acccgaggct gaccgccacc cgggcgacga tgcgggacca
     4681 gtggttgcgg gggacctcgg gcggtcggtc ggggtcgccg atctcggagc gttcgatctc
     4741 ctccagcgcc gcggtgcgat cgtcggcggg gtcggtgtgc tccatgaccg gatccacgct
     4801 atcggcgggt accctcgccg caccgacggt cgtgctcggc agggccgtcc caccgccacc
     4861 acgggaggtc gaccatgacg atcgacgagc gcgacgaccg acgcccgtca cccgcgctcg
     4921 ggttcatgac cgacctggcg tcggtggccg acatccgccc ggtggctgtc ccgctcaccc
     4981 ccggcgaccc ggcggcacgt ggaggggcgg acacgatcga tctcacggtc gccgaagccg
     5041 gcgtgggtgg acgtccgctg ctgctggtcc acgggttcac cggcgccaag gaggacttcc
     5101 gtgacgtcgt ggcgccgctg gccgcggccg gccactgggt cgtcgcaccc gacctgcggg
     5161 gccacggcgc cgggccgcac ccacccggcg aggacagcta cggcctcgac cggttcgccg
     5221 cggacctgtc cggcctggtc gacgtgctcg gctgggaccg cttcgacctg ctgggtcact
     5281 cgatgggcgg gatgatcgcc cagaaggtgg cgctcgccca tgggtcccgg atcgaccggc
     5341 tggtcctgat ggacaccgcc accggaggcg tcgacgggtc cgagcacggc gcggacccgg
     5401 acaacgcggc gctgatgcgg atcggcgtcg agctgtgccg gtccgagggg atcgccgcca
     5461 tcgccgcggt gctgcagatg ggggaccagc cgttggagag ccccgcgcac cagcggttgg
     5521 ccgagcagcc cgggtggacc gagtgggagt cggggaagtt cctcgcgtgc tcgggcgaca
     5581 tgtggtgcgc gatggtcgag gagtttctct ccaccccgga ccggatggat ctgctggcgt
     5641 cgctgcccat gccgacgctg gtcgtggtcg gcgagcagga cagggcgttc cgggcggtgt
     5701 cggcacgcat ggccgaggtc atttccgacg ccaggctctc ggtgatcccc gatgccggtc
     5761 acagccccca gttcgagaac ccgtcggcct ggtacgcggc ggtggcgggc ttcctggcgc
     5821 gctgagccgg accccggtga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 ngtgcggccc aggtacgccg ccagtcgggt ggcggcgttc gccccggcgg gtgcgtcctg
     6001 ctcggccctg aagatcgacc cgtcctcggt gcggaacgac ggcgggatcg cccggtgggc
     6061 gttgtcgagc agcagctcgg ccagctcggg atcgaggtcg ttgtccaggt cgagcgcggt
     6121 ggccaggtcc caggcgtggg tgaacgtgtc ggtggtggcg atgccgagca gcgccgcggc
     6181 gggcatctcg ccgaagccgg gatcgacggt gcgttcgagg gccccgtcgg cctggaacgc
     6241 ggccaggcac gccgcggcgg cgtcggcgta ccgggcgcgg aagtcgtcgg ccgacgcgcc
     6301 gtcgccggtg tcggtgagct cgacgccgtc gatggctgcc cgggcccagt cctgggtgcc
     6361 gaccatgtga tcgatgagct ggccgacgtt ccactcggcg cacggcgtgg gtgcctgcaa
     6421 ctggtcggcg ccgacctggg cgagcagccc ggcggcgatg tcctgggcct gggcgagggc
     6481 ggtgggttcc atggcgatcc tcctgcgatg gactggcggc ggacgggcac cgcgacgcta
     6541 ccggaggggt cgtcgggccg ctccggggat cgtcagtggg tcttctccat gaagcggtcg
     6601 acgtcgacga ccacccgggt caccttgccg gccgccacca ggccgcgctc gtcgcgggcc
     6661 gagacgtgga acagcagccg acggccctcg accttctcca ggttggcctc ggcgcgcacc
     6721 acacccccga tggcggtggg ggagatgtgc tcgagctgga cccgcatccc gacggtggac
     6781 gatccctcgt cgaggtggcc gtcgagggcg cgcaccgtgg cctcttcgac caacgcgagg
     6841 atgcgggggg tggccagcac cgggacctca ccggatcgca tggcgatggc ggtgtcggcg
     6901 tccgacacgg tcagatcgat ggcggcggac agcccggtgc ggatcgacac ggccgatacg
     6961 ataagcaccc cgccgggcgg tccccaagct ccggccggtc ccgtgggcgg gtccggggca
     7021 tgctggtggc cgtcgtcccc gctcctcggg gtccgttgcg tgaccgctgc ggacccgtcg
     7081 accgggccgc ggcaccgtcc tctggccgtc aaggagatcc ccgtgcccga tgcccccacc
     7141 gaccgcagct ccgagccggc cctgctcgat caggccgtcg tcgaacgggt gctcggggcc
     7201 ggcctcgccc ggggggcgga cttcgccgag gtgttcgtcg aggaccgtcg gtcgtcgtcg
     7261 gccctgctcg acgacggccg ggtcgaggag ctgacatcgg gccgcgaccg cggggccggc
     7321 atccgggtcg tggtcgggga ctcgaccggt ttcgcccaca cctcggacct gtcggaggcc
     7381 gggttgatcg ccgcggccga ggccgccgcc gcggcgtcgc ggggagccgg cgggtcgacc
     7441 cgggtggtcg acctcgacag ggtcgacgcg ccgccgccca acgctgtggc gatcctcccc
     7501 ggcgacgtgc ccaaggcccg caaggtggag ctgctgctga ccgcggacgg ggccgcccgg
     7561 gccgccggcg gggcgatcac tcaggtgtcg gcgcgctacg cggatggccg ccggcggatc
     7621 ctggtcgcca acagcgacgg cgtgctcgcc tccgacgacc aggtccggac gatcttctcg
     7681 gtgagctgcg tcgccagcgg cgacaccggc atgcagaccg gtcgcgagtc ggtcggccac
     7741 acggtcggct tcgagctgtt cgacggcgtc gaggtggcgg acctggccga gcgggcggcc
     7801 cggcgggcgc tcaccaagct ggcggcgcgc ccggcgccgt cgggcgagat gaccgtcgtc
     7861 atcggcccgg gtggtggcgg cgtgatgttc cacgaggcgt gcggtcacgg actcgaggcc
     7921 gacctggtcg gcaagggtgc gtcggtgttc gccggccggc tcggcgagca ggtcgcgtcg
     7981 ccgctggtca ccctggtcga cgacgggacg atgggcggcg agtgggggtg cttcgccgtc
     8041 gacgacgagg gccggccggc gcagcgcaac gtgctgatcc aggacggcgt gctcgtcgac
     8101 tacatgtggg acgggctgcg ggcccgcaag gaggggcggg cgtcgagcgg caacggccgg
     8161 cggcagagct accagcacct gccgatggtg cggatgacca acacgtacct gctgggcggg
     8221 acctcttcgc ccgacgacat cgtcgccgac acgccgtcgg gggtgtacgt ggcgcacctc
     8281 ggcggcggtc aggtcaacac cgcgaccggc gacttcgtct tcggcatgac cgaggcgtac
     8341 ctgatcgagg acggtcgcat caccgagccg ctgcgcgagg gcaacctgat cggcaacggc
     8401 ccggccgtgc tggccgccat cgacgcggtc ggcgacgact tcgccatggg tgcgccgggg
     8461 acctgcggca aggacggcca gggggtcccc gtcggcgacg gggtccccac gctgcgggcg
     8521 cacgggctga ccatcggcgg aaccgccgcc tgacccgagc catcggccgt cacccggccg
     8581 ttgttgtagt cagcggtcgc tggggtggct tgtgggctac aagtacggcg gcgccgggcg
     8641 ttgttgtagc cggcggtcgc tggggtggcg tgtgggcgac aagttcggcg gcgccgggcg
     8701 ttgttgtagc cggcggtcgc tggggtggcg tgtgggcgac aagttcggcg gcgccggggc
     8761 gctcagagcg ggagggagtt tccgccgaac gacgtctgca ggcggttcat ccactcggtc
     8821 cacacgccgg tgacctgcag cacgccgagc acgacgagca tcgcgccgct gccgaccatg
     8881 agcgctcggt agtggttctt gatcacgccg aacaccgtca tcgcccggtt gaacgccagg
     8941 ccggagatca gcagcgggat ccccaggccc aggcagtagg cgagcgacag gacggcgccg
     9001 cgccacgccg aggcctggtc gctcgaggcc gacaacccga gcaccacgcc cagggtcggt
     9061 ccgatgcacg gcgtccagcc gagggcgaag gtgagcccga tgatcggcgc agccagcagc
     9121 ccctgacccg ggatccggtg gatgcgcagg tcccggttca gcaggtcgct gcggctgaac
     9181 gcaccggcga ggagcagccc caggaagatg gtgacgacgc cgaggatctg ggtcagcccg
     9241 acggcgtggc gttgcagggc gtcgccgaag ctcccgaacg cggccccgaa cgagacgaac
     9301 acgacggcgg tgccggccac gaacccgagc gttccgatga cgacccgccg catggtgtgc
     9361 acgtcggtcg aggatccacc ctcgctcagc tccgccaccg acacgcccgt cacataggac
     9421 agataggcgg gaaccagagg caggacgcag ggcgacagga acgacacgac gccggccagg
     9481 acggcgatcg gcagcgcgat cagcagcgtt ccgtcccgga ccaggtcctg ggaggcgacg
     9541 acaacggaga gggccccggt cacgacgacc ggatctcctc gagtgcggag cggacctcgg
     9601 cgctgtcgaa gtcgacagct ccggggaagg tcgccgccac ctctccgtca cgatcgagca
     9661 gcactgtcgt cgggacgccg agcgccttga acgagcgctt gaactcgttg cggcggtccc
     9721 gccacagctg ggtgctcagc ccgtggcgct cgatcttggc gtcgatctcg tcgtcgaccg
     9781 acgcggcgtc gaggttgacc gccaccacct cgagaccctt tgcgtcctcc gacgacgaga
     9841 acttctggag gccgctgagc acctcgtcgc actccttgca ccaggtcgcc cagctgacca
     9901 ggaccacggg ctcgccccgg aggtcctgga ccgacacgtc ctggccggag cgcaggtcct
     9961 cggcgacgta ggcgtcgtcg gaaccgccac ccccgcaggc tgtgccgagc accgagatgg
    10021 cgacggctgc gatgagcaga ccgacggtgg cggtggctcg gtgggctcgt gcggcggtca
    10081 ttccttgacc acgatctcgt aggtctgccc gtcggcgagg gggcagaagc cggcgaagcg
    10141 cccgggggac ccgtaggtga acgagaacga gttgcccggg cccaccaggt agggacccac
    10201 ccactgcggc accgcgtcct tgttctcgat gaagagggtg tcgccgacgc gcagctccat
    10261 gtagctgggc atcatgacga ccttctcgcc ggcgttcacc cggtcctggg tcccggcctc
    10321 gacgaccagg tggaccgtcc gcggctcggg tccgctgtcg cggctcacga ccagccaggc
    10381 gaccccggcg gtcaacaggg cgagcaccga gatggtcgcg gcgatcacca acccacgatg
    10441 gcgtcggcgc ggctcggccc cggccggggc ccccggacct gtgtccggga gccccgacga
    10501 ggtcggatca gcggtgtctc cagcagtcgg atcagccgtc ggatccgagg tcgggtcaag
    10561 gggcgttgtc acggacttcc gcctcgacga cgacgtcgcc ggccttctcg aaggtcagcg
    10621 tcaccttcac cttctcaccg acgaccagcg gcttggccag ctccatcaac atgatgtggt
    10681 agccgccggg ctcgagggcg acggtcgtgc cggcggcgac ggggatcttg tccacctcgg
    10741 ccatcgtcat catcccgccg ccgcccatcg cggtggtcga ggaaccgtgg gcgtcgcccg
    10801 actgtccctg acccatcccc tgctcgggcg cggtggtgct cgacattccg ccctcggccg
    10861 ccttggtctc gtgcagctcg gtcttcttgg cgaccgacgg gtcgaccgag gcggcgacca
    10921 gggcgtcgtc ggagtcggag tcgttggtga tcttcaggta gacggctccg gcgctggcca
    10981 ccgcgggtga ggtgcgggcc cagacccccg aggtgctgat cgcggcgtcg tcggcgggct
    11041 ggctggtgga cgcctcgggt cgggtcgtcg atgacgatga ctctttgccg tcgtcgccac
    11101 agccgacgag gaacaccccg gcggtcaggg cgagggccat cggtgccgcc acggagcggc
    11161 ggattcggtc ggtgtgcatg tcaaccctcc aggagtgtgt tgaggtcctt ggtcatgtgc
    11221 tcgctgtcga agccgaacgg ccactcgacg gcgaccttgc cggtgtcgtc gatgacgtag
    11281 gtcaccgcgg tgtgcgacac ctcgtagtcg gtggcacccg gtgggtgcgg ggcgatctcg
    11341 tagcggaccc cgaaggcctc ggtggcagcg gtcagggcct cgggcgtctc ggcccgcagg
    11401 gccatcgagc ggtcgaagaa gtggctgagg tactcgctca gcagctcggg tgtgtcgcgc
    11461 tctgggtcga cggtcaccat gccgaccgtc acccgcttcg cgtcggcctc atccaggtcg
    11521 ccgagagcga cctggatgtc gctcagcgtc gtcgggcaga tgtcggggca ggacgcgtag
    11581 ccgaagtaga cgacgtacag atcgcccttg ggcggggtca tggtgaccga ccggggcgtc
    11641 gctccggagg tgtcggtcag ggtgacgtcg ccgaccgcga gcggcgggtt tcggacggcg
    11701 cccagcaggt cgccggaacc cgaggcgtcg tcgtcggtgg tgtcgctgcc gcaggcggca
    11761 cctaccagcg tgagagccac gagcagggcg acgatgcgac cggtccgcac ccgtcgccgt
    11821 tgacggaagt tatcggacat agagatatca aaccacagat gtatcaccag ttccgtgctg
    11881 ggccgtggtg acttgggtca tgcgtgtggg gttcgggccc gcgggctcgg cgttgttgta
    11941 ggtggcggtc gctgtggtgg cgtgtgggct acaagttcgg cggggc
//
LOCUS       DAOQOD010000096        11938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1907_length_11938_cov_11.957334, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000096 DAOQOD010000000
VERSION     DAOQOD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_11938_cov_11.957334"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLZ72_10210"
     CDS             <1..321
                     /locus_tag="PLZ72_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36712.1"
                     /translation="LVGNAAIPAYCASKSGLLGVTRALAHQLGPEGIRVNAVCPGFVE
                     TPMLQIALDVPGLREEMEAEAPLGRLARPDEIGHAVAFLLSERASFITGTHLVVDGGA
                     TAVL"
     gene            426..>1240
                     /locus_tag="PLZ72_10215"
     CDS             426..>1240
                     /locus_tag="PLZ72_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36713.1"
                     /translation="MPAVTLPPRRSADRRSVTRGRTVRRSVAAILVTAVAASGCVGTD
                     GGAVGRRDDRREVRAEGGSDGSGRAPDGDGPTSDTDAPTTTTTEPRPPADTLTMKKFT
                     RISGDISPKSVVASGHGVVTAQNMMYTHTVTAYDSDGKLIATVPDSVTLSDFGIDKPG
                     VYKGAPVEAAFTPDGGHVYVSNYSMYGPGFSEGKDKCSPGDGTTSSYLYRISTKSWKI
                     DQVIPAGAVPKYVAVTPDGTRVLATNWCTWDLTIADTETGKVLKTVKIDGRYPR"
     assembly_gap    1241..1340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1446..2411
                     /locus_tag="PLZ72_10220"
     CDS             1446..2411
                     /locus_tag="PLZ72_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HNA36714.1"
                     /translation="MAELVIARNPDGDSRLPYLLRLPLGEAGLVLKARETWPRTAKVY
                     CHPSDDWPDEHELEIVERVAVRSCVRRGAAVDLVLDRGRENRSQFVFARARGREVIFW
                     QTARTNRQARPAVSVPTRRASGRTLEILVDSHERYAWKFNEQQATTRRQALAAGDYAV
                     ESGGSVLAAVERKSMADLVGTMSGGRLRYLLAALADVPSAALVVEDRYSSVFKQRHVA
                     PSTIAEGLAEAQVRFPTVPIVFCETRQLAQEWTYRFLGAALDHADEDEVGSARTADIP
                     TAAPVAPAAPTAAAVRAWAREEGIEISDRGRVPGAVVEAYRLAHP"
     gene            2552..4873
                     /locus_tag="PLZ72_10225"
     CDS             2552..4873
                     /locus_tag="PLZ72_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNA36715.1"
                     /translation="MADSVGEVMGESRPVRHRIRSGVVVAATLCVAVLAGCAARPPLP
                     DVGNQSPVDRSGYRVLAGVEQLYVLGALTGDAIEVRDGTDVVGGGTADRLGSLAVRGL
                     HQGRTYTVLNVTSGDSRPARILRADEHPPQSFYDSTVMHEGLNYIPMRDGITLAATVR
                     PPLGLSLADGPFPTVIEYSGYQVAAPGDPLLNKLGGLFGLPRDPMAPGGETDVGSLLV
                     RLAGFATVSVQIRGSGCSGGESDLFDLPTNLDGYDAIETVAAQPWVSNGIVGMVGISF
                     SGFSQIGVAATHPPHLAAIAPMSFTGNLYEVAHPGGIFNDGFGSTWMAERVANARPAP
                     DPGALPYANGLVATDAQCRENQKLRLQTRDGNDLIRSNELFTDIYRRRDFPGWMRQIR
                     VPTFASLQFDDEETSSYAIQSVSGLLGANDRVFLNLSSGHHRDAVTPDTITEYFEFLD
                     IYVARRPPEPKLLVNLVSDIIFGTGSAVPRFPDTVFMPLEAARARVEARPRVRLLTEL
                     PAGSAEGHNPGARWDVRVPTYPVPGSTEQTWYLGPQGALTSVPPPVGAAASYRPDVVG
                     RRHDPGPAVVDDPERFVWTPVAADASLGFVTAPLDQDVVVVGPVGARIALSSSAADTD
                     LAVTVTEVRPDGQEMLVGTGVQRASMRHVDADRTTATRPALTFDRVEPLRGAGAVDVV
                     PVQVMPMSHVFRAGSRIRIVVAGVNGDRERWTFDSVDPADRSTVDTVHLGGATPSSVT
                     FTVAPLGPAPAERLPCPAAAKPCRPWIPAGNGG"
     gene            5076..6245
                     /locus_tag="PLZ72_10230"
     CDS             5076..6245
                     /locus_tag="PLZ72_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36716.1"
                     /translation="MVRRNSVRLAVALLVMSVVAPFVSSGTAGAIGSPPSTTIGARLR
                     FATTNGDILAITKVSGTSRNLIAFGGNFTKVITPDGVSRDAKNFAVVDEFTGALVYGG
                     NANNYVRAITSLNGTVYVGGDFTSFGGVARPRAAALNPSFAVTGWNPQPGSRVRALTV
                     ANGNVVYGGDSSVVRGTNPTTGGSVWSQSISGGSVRSLGTAPNGTLYIGGMFEKYGTL
                     TRHGLIKANPANGAPDTAFNAKLRADTGVGSHGSYDGQAGNVINFNADGSRLLLGVAG
                     YGSDEFKTLNPNTGSLYWGLVLPGDCQGVGVVGSTYVLGYHRHDNNPWPYFGAQVEAS
                     NGRLTTWNPGLTGYQSNVDGGNQGVQAVYVDSANRRLFVAGAFTKPVQSLAAYSW"
     gene            complement(6328..>6956)
                     /locus_tag="PLZ72_10235"
     CDS             complement(6328..>6956)
                     /locus_tag="PLZ72_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNA36717.1"
                     /translation="GLVGDVQRHRFEIFKSLTILRQLSLDPGLIDAEHDAVGSAKLDR
                     LLEDLTQVVAEGHRALVFSTFTGFLSRVRARLDEAGIGYAYLDGRTRNRERPIAAFKD
                     GDVPVFVISLKAGGFGLNLTEADYCFVLDPWWNPAAETQAVDRAHRIGQDNTVVVYRY
                     VSTGTIEEKVMELKARKAALFSSVMDADDAMTGALDADDIRALIDLPS"
     assembly_gap    6957..7056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7057..9767)
                     /locus_tag="PLZ72_10240"
     CDS             complement(<7057..9767)
                     /locus_tag="PLZ72_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNA36718.1"
                     /translation="MSGPVPRLELSDPGLFAGYDPRSVERGIRYAADGRVELIDVGDG
                     WATADVAGTRPTPYEVDISWSVTARGVSVADDCTCPLGGSCKHAVAVIVEVARHTNAS
                     VGIAGGSRRTTSATGLAASRPGPTWRTAFDRLRSDDERAHTPLALQFTISRPRATTYD
                     PAPAAQVLVRPMRPGAKGKWVKSGAGWREVTSGYAWELRSVDPDQLAAIQRLAAGYRE
                     LSYSSGSTAVPLERFGPGAWPVLRAAVDAGVALIDGRGQETVTLASDPATLAVDLVAT
                     DDGLAVSTGFVPGAGTTVHGEDQLFTIGRPPHGLFTVHDTRLELIPLDRPVPAALAEL
                     IGGPPVAVPESDLDEFLDEVHPRLSRVATVGSSDGSITVDATELDRLVVRIDHHAVDA
                     ARLEWWVRYRRGERISTFPLRHPGGVARDRSAERDLVQDVELPTDLLDGLVDVSGRPA
                     DVSVHGPDAVTLLTRVVPWLLDRGQVDVELRGDAPELREASQDPLIALNVTDPDASDG
                     SGTDWFDLSVDVSIDGEPIEFASLFTALATGASVLILPSGTWLNLDRPELDRLRDLID
                     EARGLLEVDDSGSIRLNPFQTDWWEELASLGVVESQSRRWADNVARMQALSAPEPVDP
                     PAGLAAELRHYQQDGLDWLAFLHRNRLGGILADDMGLGKTVQTLGLFLHVLDQDPDAR
                     FLVVAPTTVVANWHRETDRFAPGVEVVTIRETEARRGTSLIEAVGDARIVVTSYALFR
                     LEFDQYRSIDWEMLVLDEAQFVKNHTSKTYQCVRRLEATTKLAITGTPIENSLMDLWS
                     LLSIVAPGLYPDPRRFSEVYRKPIESGRAPDRLDTLRRRITPLMRRRTKDEVLTELPP
                     KVEQTVDVELSARHMRIYQTQLQQQRKKVLGLVGDVQRHRFEIFKSLT"
     gene            complement(9812..10618)
                     /locus_tag="PLZ72_10245"
     CDS             complement(9812..10618)
                     /locus_tag="PLZ72_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36719.1"
                     /translation="MLTLGGGLIGSSVAIAACSDGGSSATSASTASTASTTSTTVAGA
                     SSTTTAGAPSAAGTPPTTVFTAADFEPLDVCRVLPELMAGPFPTKVQMERRDITEGRS
                     GEPLRVGIRVVDRSCTPIPGAAVEIWHCDVDGDYSSYLDGVTPDDDGETTTFLRGTQT
                     TNADGIVEFVTIWPGWYPGRAIHIHSRVHVEDRTVLTTQYLFDDDLNTEVTATGPYAQ
                     HGPPDTPNADDSVADDPAAQGLLFSVADDPALKGRRALIVVGVDPAAESA"
     gene            10859..11122
                     /locus_tag="PLZ72_10250"
     CDS             10859..11122
                     /locus_tag="PLZ72_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36720.1"
                     /translation="MAPPDRSMGETGQPTRIRATAVVVIVGTVVLLAGACAPPSGPGS
                     TTTVNTSPTAVIVADPTSGPAPLAVSFSGLSSTQPGGTITGHS"
     gene            11138..11938
                     /locus_tag="PLZ72_10255"
                     /pseudo
     CDS             11138..11938
                     /locus_tag="PLZ72_10255"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
BASE COUNT         1688 a         4143 c         4209 g         1698 t
ORIGIN      
        1 ctggtcggca acgccgccat ccccgcctac tgcgcgtcga agtcggggct cctcggagtg
       61 acccgggccc tggcgcacca gctcgggccg gaggggatcc gtgtcaacgc agtgtgtccc
      121 ggcttcgtcg agaccccgat gctgcagatc gccctcgacg tcccgggtct gcgcgaggag
      181 atggaggccg aggcgccgct ggggcgactc gcccgacccg acgagatcgg tcacgcggtt
      241 gcgttcctgc tgtcggagcg ggcgagcttc atcaccggca cccacctggt cgtcgacggc
      301 ggggcgaccg ccgtgctgtg accgcgggcg ggccggtcgc gcatcggcgt ctgtcagcag
      361 gcgattgtgc gtgccgagtg ggaagcgcgc gtcgtgagct cccgagcgtg tggaacgctg
      421 tcgggatgcc cgctgtcacc ctgccacccc gtcggtctgc tgaccgccgc tcggtgaccc
      481 gggggcggac cgtccggcgc tccgtcgccg ccatcctggt gacggcggtg gcggcgtcgg
      541 ggtgcgtggg caccgacggc ggtgccgtcg gccgtcgcga cgaccgacgg gaggtccgcg
      601 ccgagggcgg atccgatgga tcggggcggg ccccggacgg cgacggcccg acgtcggaca
      661 ccgacgcccc gaccaccacc acaaccgaac cgcgcccgcc cgccgacacg ctcaccatga
      721 agaagttcac ccggatctcc ggggacatct cacccaagtc ggtggtggca tcgggtcacg
      781 gggtcgtgac ggcccagaac atgatgtaca cccacaccgt cacggcctac gactccgacg
      841 ggaagctcat cgccaccgtc cccgactcgg tgacgctcag cgacttcggc atcgacaagc
      901 ccggcgtcta caagggagcg ccggtcgagg cggcgttcac tcccgacggc gggcacgtct
      961 acgtgtcgaa ctactcgatg tacggacccg ggttcagcga gggcaaggac aagtgctccc
     1021 ccggcgacgg caccaccagc agctacctgt accggatcag cacgaagtcg tggaagatcg
     1081 atcaggtcat ccccgccgga gccgtcccca agtacgtggc ggtcaccccc gacggcaccc
     1141 gggtgctggc caccaactgg tgcacgtggg acctgaccat cgcggacacc gagaccggca
     1201 aggtgctgaa gaccgtcaag atcgacggcc gctacccgcg nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn gcaccatcga cgtgtgggac gaggtgccgg ccgacggggc
     1381 cggggacggg accggaacga ccacgaccgg tcccaccgca gccgccgcga cctcggaggg
     1441 atccggtggc tgagctggtc atcgcccgca accccgacgg cgactcgcgc ctgccgtacc
     1501 tgctccgcct gcccctcgga gaggccgggt tggtgctcaa ggcccgggag acctggcccc
     1561 ggaccgccaa ggtctactgc catccctccg acgactggcc cgacgagcac gagctcgaga
     1621 tcgtcgagcg ggtggcggtc cgctcctgcg tgcgccgggg cgcggcggtc gacctggtgc
     1681 tggaccgggg ccgcgagaac cgctcgcagt tcgtgttcgc ccgcgcccgg ggccgcgagg
     1741 tcatcttctg gcagaccgcc cgcaccaacc gccaggcccg gcccgcggtg tcggtgccca
     1801 cccggcgggc gtcgggtcgg accctcgaga tcctggtcga cagccacgag cgctacgcct
     1861 ggaagttcaa cgagcagcag gcgaccacgc ggcgccaggc cctcgccgcc ggcgactacg
     1921 ccgtcgagtc gggcgggtcg gtgctcgccg cggtggagcg caagagcatg gccgacctgg
     1981 tcgggaccat gtccggtggc cggctccgct acctgctcgc ggccctggcc gacgtcccct
     2041 ccgcggcgct ggtcgtggag gaccgctact cgtcggtgtt caagcagcgc cacgtcgctc
     2101 cctcgaccat cgccgagggc ctcgccgagg cccaggtccg gttcccgacc gtgcccatcg
     2161 tgttctgcga gacccgacag ctggcacagg agtggaccta ccggttcctc ggcgccgcgc
     2221 tcgaccacgc cgatgaggac gaggtcggct ccgcccgcac cgccgacatc ccgaccgcgg
     2281 ctcccgtcgc gcccgcggca ccgacggccg ccgcggtccg ggcctgggca cgcgaggagg
     2341 ggatcgagat ctcggaccgg gggcgggtac cgggagccgt cgtcgaggcc taccggctcg
     2401 cccacccctg acgtcgccgc cggtccgtgc cgcgtccatg cgggaccgac gccgtgtggc
     2461 acatgtcagg gtgaccggtg agcgggcgga ggtctacgtt ctgcctgccg tcgcgggacg
     2521 gcccggtcgg tgcggtacga gccgctcgac catggcggac agcgtgggag aggtcatggg
     2581 ggagagtcgg ccggtgcgtc accggatcag gtcgggtgtc gtggtcgccg cgacgttgtg
     2641 cgtggccgtg ctggccgggt gcgcggcgcg accaccgctg cccgacgtcg gcaaccagtc
     2701 tccggtggac cgcagcgggt accgggtgct ggccggcgtc gagcagttgt acgtgctcgg
     2761 cgcgctgacc ggcgacgcca tcgaggtccg tgacggcacc gacgtcgtcg gtggaggaac
     2821 cgccgaccgc ctcggcagcc tggccgtccg tggactgcac cagggccgga cctacaccgt
     2881 gctcaacgtg acctccggcg actcccgacc ggcacgcatc ctgcgcgccg acgagcatcc
     2941 gcctcagtcg ttctacgact cgacggtcat gcacgagggc ctcaactaca tcccgatgcg
     3001 cgacgggatc accctggcgg cgaccgtccg tccgcccctg ggcctgagcc tggccgacgg
     3061 cccgttcccg accgtgatcg agtactcggg ctaccaggtc gcggcacccg gcgatccgct
     3121 gctcaacaag ctgggtggcc tcttcggcct gccccgcgac ccgatggcgc cgggcggcga
     3181 gaccgacgtc ggcagcctgc tcgtccgcct ggccggcttc gccacggtgt cggtgcagat
     3241 ccggggcagc ggctgctccg gaggagagtc cgacctgttc gacctgccga ccaacctgga
     3301 cggctacgac gcgatcgaga ccgtggcagc ccagccgtgg gtcagcaacg gcatcgtcgg
     3361 catggtcggc atctccttct cggggttcag ccagatcggc gtcgccgcca cccacccccc
     3421 gcacctggcc gcgatcgccc ccatgtcgtt caccggcaac ctctacgagg tggcccatcc
     3481 gggcggcatc ttcaacgacg ggttcggatc cacgtggatg gccgaacggg tggccaacgc
     3541 ccgaccggcc ccggacccgg gtgctcttcc gtacgccaac gggctggtgg ccaccgacgc
     3601 ccagtgccgg gagaaccaga agctgcggct gcagacccgt gacgggaacg acctgatccg
     3661 cagcaacgag ctgttcaccg acatctaccg ccggcgggac ttcccgggct ggatgcgcca
     3721 gatccgggtg ccgaccttcg catccctgca gttcgacgac gaggagacca gctcctacgc
     3781 gatccagtcc gtctccggcc tgctgggggc caacgaccgg gtgttcctga acctctccag
     3841 cggtcatcac cgcgacgcgg tcacccccga cacgatcacc gagtacttcg agttcctcga
     3901 catctacgtc gcccgacgcc ccccggagcc caagctcctg gtcaacctgg tgtccgacat
     3961 catcttcggg accggcagcg cggtgccgag gttccccgac acggtgttca tgccgctcga
     4021 ggcggcgcgg gcccgggtcg aggcccggcc ccgggtccga ctgctgaccg agctgcccgc
     4081 cgggtcggcc gagggccaca accccggtgc acgctgggac gtgcgggtcc cgacgtaccc
     4141 ggttcccggc tcgaccgagc agacctggta cctcggaccg cagggggccc tgacgtcggt
     4201 cccgccgccg gtcggcgcgg cggcgtcgta ccgccccgac gtggtcggtc gccgccatga
     4261 ccccggaccc gcagtcgtcg acgatcccga gcgattcgtg tggacacccg tcgccgcaga
     4321 cgcctcgctg ggcttcgtca ccgcccccct cgatcaggac gtcgtcgtcg tcggacccgt
     4381 cggggcgcgg atcgccctga gctcttcagc cgcggacacc gacctggcgg tcaccgtcac
     4441 cgaggtccgt cccgacggcc aggagatgct ggtggggacc ggggtgcagc gcgcctcgat
     4501 gcgtcacgtc gacgccgacc gaacgactgc gacccgtccg gctctcacct tcgaccgggt
     4561 cgagccgctg aggggcgccg gcgccgtcga cgtcgtcccg gtgcaggtca tgccgatgtc
     4621 gcacgtgttc cgggccggga gccgcatccg gatcgtggtc gccggggtca acggcgaccg
     4681 ggagcgctgg acgttcgact cggtggaccc cgcggaccgc agcacggtcg acaccgtcca
     4741 cctcggcggg gcgaccccct cgtcggtgac gttcacggtg gcgccgctgg gacctgctcc
     4801 cgcggagcgg ctgccgtgcc cggcggcggc caagccctgt cgcccctgga tccccgcggg
     4861 caacggcggc tgagctgacg cctctggcgg tccggcgatt gccgcggagg atgatggcgc
     4921 tcgcgcgatc cgtgcacatc ctccgccggc cgagagaaat cctgtcgctc agggcagaaa
     4981 tggtcccttc gagcttgtgc tgagtgcgtc cattgggaac gatggggtgc ggtcgggcgt
     5041 ggcggatgtc cgacagcgag gggaggagat tccacatggt tcgtcggaac agcgtccggt
     5101 tggcggtcgc gttgctcgtg atgtcggtcg tcgcaccgtt cgtgtcgtcc gggacggccg
     5161 gggcgatcgg ttcgccaccg tcgaccacga tcggggcccg gctccggttc gccaccacca
     5221 acggcgacat cctggccatc accaaggtct ccggcacgtc gcggaacctg atcgccttcg
     5281 gcggcaactt caccaaggtg atcacccctg acggcgtcag ccgcgacgcc aagaacttcg
     5341 ccgtggtcga cgagttcacc ggtgcgctgg tctacggcgg caacgccaac aactacgtgc
     5401 gggcgatcac cagcctcaac ggcaccgtct acgtcggcgg cgacttcacg tccttcggcg
     5461 gcgtcgcccg gccccgggcc gcggccctca acccgagctt cgccgtcacc ggctggaatc
     5521 cccagcccgg atcgcgggtg cgggcgctga cggtggccaa cggcaacgtg gtgtacggcg
     5581 gagacagcag cgtcgtgcgc ggcaccaatc cgaccaccgg tggcagcgtc tggtcccaga
     5641 gcatctcggg tggctcggtc cgatcactcg gcaccgcccc caacggcacc ctgtacatcg
     5701 gcggcatgtt cgagaagtac ggaacgctga cccgccacgg cctgatcaag gccaaccctg
     5761 ccaacggggc gcccgacacc gcgttcaacg ccaagctccg ggccgacacc ggggtcggct
     5821 cccacgggag ctacgacggt caggccggca acgtgatcaa cttcaacgcg gatggcagcc
     5881 gactgctgct cggcgtcgcc ggatacggct ccgacgagtt caagaccctc aaccccaaca
     5941 ccggctcgct gtactggggt ctggtgctcc cgggcgactg ccagggcgtc ggtgtggtcg
     6001 gatcgaccta cgtgctcggc taccaccgcc acgacaacaa cccgtggccc tacttcggtg
     6061 cccaagtcga ggcgtccaac ggtcggttga ccacctggaa tccggggctg accggctacc
     6121 agagcaacgt cgacggcggc aaccagggcg tgcaggccgt gtacgtggac tcggcgaacc
     6181 ggcgtctgtt cgtcgccggt gccttcacca agccggtcca gtccctcgcc gcctactcct
     6241 ggtgacccag gtttctgacg acaagtcacg acaactgcgg tagtcagttg tcatgagaat
     6301 cgggttgtcg atcgacctgc cggccgctca ggacggcagg tcgatcagcg cccggatgtc
     6361 gtccgcgtcg agggcgccgg tcatggcgtc gtccgcgtcc atcacgctgg agaacagagc
     6421 ggccttgcgg gccttgagct ccatgacctt ctcctcgatg gtcccggtcg acacgtagcg
     6481 gtacaccacc acggtgttgt cctgaccgat gcggtgcgcc cggtccacgg cctgcgtctc
     6541 ggcggcaggg ttccaccacg ggtcgagcac gaagcagtag tcggcctcgg tcaggttgag
     6601 gccgaagccg ccggccttga ggcttatgac gaacaccggg acgtcaccgt ctttgaacgc
     6661 cgcgatcggc ctctcgcggt tccgggtacg gccatcgagg taggcgtagc cgatcccggc
     6721 ctcgtccagg cgggccctca cccgactcag gaagccggtg aacgtgctga acaccagcgc
     6781 acggtgacct tcggccacca cctgggtgag gtcctcgagc aggcggtcca gcttggcgga
     6841 gccgaccgcg tcgtgctcgg cgtcgatgag ccccgggtcc aggctgagct ggcgcaggat
     6901 cgtgagcgac ttgaagatct cgaagcggtg gcgctgcacg tcgccgacca ggccgannnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtga gcgacttgaa gatctcgaag
     7081 cggtggcgct gcacgtcgcc gaccaggccg aggaccttct tgcgctgctg ctggagctgg
     7141 gtctggtaga tgcgcatgtg gcgtgcgctc agctcgacat cgacggtctg ctccaccttg
     7201 gggggcagct cggtcagcac ctcgtccttg gtgcgtcgcc gcatcagggg cgtgatccgt
     7261 cgtcgcagcg tgtcgagccg gtccggggcc cgaccgctct cgatgggctt tcggtacacc
     7321 tcggagaaac gtcgggggtc ggggtagagc ccgggggcca cgatcgacag gagcgaccac
     7381 agatccatca ggctgttctc gatcggggta ccggtgatgg cgagcttggt ggtcgcctcg
     7441 aggcgacgca cgcactggta ggtcttcgac gtgtggttct tgacgaactg cgcctcgtcg
     7501 aggaccaaca tctcccagtc gatgctgcgg tactggtcga actccagacg gaacagggcg
     7561 taggacgtca cgacgatccg ggcgtcgccc accgcctcga tcaacgaggt gccgcgccgc
     7621 gcctcggtct cccggatggt gaccacctcc acgcccggag cgaaccggtc ggtctcacgg
     7681 tgccagttgg cgaccaccgt ggtgggggcg acgaccagga accgagcatc ggggtcctga
     7741 tcgagcacgt ggaggaacag cccgagcgtc tggacggtct ttcccaggcc catgtcgtcg
     7801 gccaggatcc cgcccaggcg attgcggtgc aggaacgcca accagtccag gccgtcctgc
     7861 tggtagtggc gcagctcggc ggccagtccc gcagggggat cgacgggttc gggggcgctg
     7921 agcgcctgca tgcgggcgac gttgtcggcc cagcgccgtg actgggactc gaccactccc
     7981 agcgacgcga gctcctccca ccagtcggtc tggaacgggt tgagccggat ggagccggag
     8041 tcgtcgacct cgagcagccc ccgagcctcg tcgatcaggt cgcgcaaccg gtcgagctcg
     8101 ggccggtcga ggttcaacca cgtcccgctg ggcaggatca gcaccgacgc tccggtggcg
     8161 agcgccgtga agaggctggc gaactcgatc ggttcgccgt cgatcgagac gtcgaccgac
     8221 aggtcgaacc agtcggttcc ggacccgtcc gatgcgtcgg ggtcggtcac gttgagcgcg
     8281 atcagtgggt cctgggaggc ttcgcgcagt tcgggggcgt cgccgcggag ctcgacgtcg
     8341 acctggccgc gatcgagcag ccacggcacc acccgggtga gcagggtgac ggcgtccggg
     8401 ccgtgcaccg acacgtcggc gggtcggccg gagacgtcga cgagcccgtc gaggaggtcg
     8461 gtcggcagct cgacgtcctg caccagatcc cgttcggctg accggtcgcg ggcgacgccg
     8521 ccggggtgac gcaacgggaa ggtggagatc cgctcgcccc ggcggtacct cacccaccac
     8581 tccagccggg cggcgtcgac ggcgtggtgg tcgatgcgga cgaccaggcg gtcgagctcg
     8641 gtggcgtcga cggtgatcga cccgtcggac gaaccgaccg tggccaccct gctcagccgg
     8701 gggtggacct cgtcgaggaa ctcgtcgagg tccgactccg ggacggcgac cggtggtccg
     8761 ccgatcagct cggccagcgc ggccgggacg ggtcgatcga gcgggatcag ctccagacgg
     8821 gtgtcgtgga cggtgaagag gccgtggggt ggccgaccga tggtgaagag ctggtcctcg
     8881 ccatgcaccg tggtgccggc gccggggacg aagccggtgc tgacggcgag gccgtcgtcg
     8941 gtcgcgacca ggtcgaccgc gagggtcgcc gggtccgacg ccagcgtcac cgtctcctgg
     9001 ccgcgtccgt cgatcagggc gacccccgcg tcgaccgcgg ctcgcagcac cggccaggcg
     9061 cccggaccga agcgctccag gggcaccgcg gtcgatcccg atgagtagga gagctcgcgg
     9121 tatccggcag ccagacgctg gatcgcggcg agctgatcgg gatcgacgga acgcagctcc
     9181 cacgcgtagc cggaggtgac ctcgcgccag ccggcaccgc tcttgaccca cttccccttg
     9241 gcgccggggc gcatggggcg gaccagcacc tgcgcggcgg gcgcggggtc gtaggtggtg
     9301 gcccggggtc ggctgatggt gaactgcaac gccaacggcg tgtgcgcccg ttcgtcgtcg
     9361 ctgcggaggc ggtcgaaggc ggttcgccag gtcggccccg gtcgggatgc ggccagtccg
     9421 gttgctgagg tggtgcggcg ggagccgccg gcgatgccga ccgaggcgtt ggtgtggcgc
     9481 gccacctcga cgatgaccgc cacggcgtgc ttgcaggagc cgcccaacgg gcaggtgcag
     9541 tcgtcggcga ccgacacgcc ccgagcggtg accgaccacg agatgtcgac ctcgtagggc
     9601 gttggtcggg tgccggcgac gtctgcggtg gcccatccgt cgccgacgtc gatgagctcc
     9661 acccggccgt ccgccgcgta gcggatccct cgctcgaccg agcgggggtc gtagccggcg
     9721 aacagtccgg ggtcgctcag ctcgaggcgg gggacgggac ctgacacgac cccggactgt
     9781 agcgagggac cccgacgttc ctgccagctg ctcaggcgga ctctgctgcc gggtcgaccc
     9841 cgacgacgat cagcgcccgc cgccccttca gcgccgggtc gtcggccacg ctgaacaaca
     9901 ggccctgggc ggcggggtca tccgcgacgc tgtcatccgc gttgggggtg tcgggcgggc
     9961 cgtgctgagc gtaggggccg gtggccgtca cctcggtgtt gaggtcgtcg tcgaagaggt
    10021 actgggtggt gagcaccgtg cggtcctcga cgtggacccg ggagtggatg tggatggcgc
    10081 gtcccggata ccagccgggc cagatcgtca cgaactcgac gatcccgtcg gcgttggtgg
    10141 tctgggtgcc gcgcaggaac gtggtcgtct cgccgtcgtc gtcgggggtg accccgtcga
    10201 ggtacgagga gtagtcgccg tcgacgtcgc agtgccagat ctccaccgcg gcgcccggga
    10261 tcggggtgca cgaccggtcg accacgcgga tcccgacgcg gaggggctca ccggagcgtc
    10321 cctcggtgat gtcgcgccgt tccatctgca ccttggtggg gaacggcccg gccatcagct
    10381 ccggcagcac ccggcacaca tcgagcggtt cgaagtcggc cgcggtgaac acggtggtcg
    10441 gtggggttcc cgcggcactc ggagccccag cggtggtggt ggacgacgcc ccggccacgg
    10501 tcgtcgaggt cgtggatgct gtcgaggccg tggacgccga tgtcgccgag gagccgccgt
    10561 cggagcacgc ggcgatcgcg accgaggagc cgatgagccc gccgcccaga gtgagcaccc
    10621 ggcgacggtc cagcagcggt cggcggtcgt ggggaatccg agggcctggg gagtccatgc
    10681 cggacaggtt gccccaggcg acgcgggcgg tgtcggcggt cccgcggttc ggggtcgacc
    10741 atcccggccg tgatctgacg atgcgtcagg tctgacttgc cccccggatg tgacatccgc
    10801 tacaacgtcg ccccacgtcc ggggtgatgg acgtggcagg ggcgggcgga tcggcggggt
    10861 ggcacccccg gaccgctcca tgggcgaaac ggggcaacca acgcgcatcc gagcgaccgc
    10921 ggtcgtcgtc atcgtcggca ccgtcgtgct gctggccggg gcgtgtgccc caccgtcggg
    10981 accgggttcg accaccacgg tgaacacgtc gcccacggcg gtgatcgtgg ctgatccgac
    11041 ctcgggaccg gcgccgctcg ccgtgtcgtt ctccggactg agcagcaccc agccgggcgg
    11101 gaccatcacc ggccacagct agaacttcgg tgacgggggc agctcgaacg ctgccgaacc
    11161 aacccacgtg tacgcaaacc cgggcaccta caccgcccgc ctgatcgtca ccggcaacac
    11221 cggcgcgacc gacaccgaga cgatggtgat cgaggtcgtg cgcgacctca gcggaatcgt
    11281 cctggtcagc accgccgggg cggactcggc ctcgtgcgga tccgcggccg agccgtgcgc
    11341 gacgatcggc cacggcctgg aacgggccgt ggcgaccggc cgcgacgagg tctgggcggc
    11401 ccagggcggc tacccggcgt tcaccgtgcg atccggagtc gacgtgcggg gcgggtactc
    11461 ggccgacttc acgggttccg gtgcggccac ggtggtgtcc ggttcctacg acccggcgtc
    11521 gggggtgtcg gcggcgatca tcgcccacga cgtcaccgcg ccgaccaccg tcgccggtct
    11581 gaccgccgtc gggggtgacg agtcgacctc cggtcgcacc tcgctggcgg cctacgtcga
    11641 cggctccggc gccggactca gcctggacgg cgtgaccctg accggcggtg tcagcggttc
    11701 ctcggccacc gcgctgctgg tcgacggtgc cacggtcgcg ctgaccgcgc cggtgatccg
    11761 tagcggcgac gcgaccggag ccggccagag cgcctacggc gtccgggcga tcaacggcgc
    11821 cgtcctcgac atcgacggcg gttccatcga gtcgggcgcg ggggccgcag gggcggcggc
    11881 tcccgtcgcc ccgtccgctc cggctcccgg ctgcaccggc ggcgccggcg gaaacggc
//
LOCUS       DAOQOD010000097        11858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1921_length_11858_cov_12.757858, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000097 DAOQOD010000000
VERSION     DAOQOD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_11858_cov_12.757858"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PLZ72_10260"
     CDS             complement(<1..1346)
                     /locus_tag="PLZ72_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA36721.1"
                     /translation="MTPDATGGANGHGNPAGAAAVVLGAGTAGLNAALQLARRGLQVT
                     VVESRRLGEGGARWDNGVLDWQYTRAGIAPPTPDEVHDTTGVTRLYGPADAGPVTVQS
                     PTRHADMRRLNDRLVGLCRAEGVAFVDRARDVVVSEVDGRVRALDLVGCDPDDGPSDG
                     QHGDEAGARALHLEADLFVDAAGRHGVLRSQVRDLVQWCPPVARDLLCTASQFMFRVA
                     EVDGARRFLDRRGVRAGDGVTWVGLDGGFSALAVTVQPDLERVSVLTGTIASGSWGSG
                     RTILDRARAENPWIGDPIYGGDGLIPLRRPFARLGAGGVALVGDAACQVFPAHGSGIG
                     TGLMAGAVLAEQVVAHRDPGSDEALWAYQHAFHAEHGGGLAAYDAFRRCSSAIGSDGI
                     RRLFTSGLFTAEMGGSGLDQRWSEPTPAQTVAAARAYLRDPGLARAMVPWLARCAIA"
     gene            complement(1368..1547)
                     /locus_tag="PLZ72_10265"
     CDS             complement(1368..1547)
                     /locus_tag="PLZ72_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36722.1"
                     /translation="MSVHITPEPTDEEAAAIVAAIQCGLPGGSAEVGPERVSRWRWSG
                     RWWSKPVPVRRGRPG"
     gene            complement(1573..3123)
                     /locus_tag="PLZ72_10270"
     CDS             complement(1573..3123)
                     /locus_tag="PLZ72_10270"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNA36723.1"
                     /translation="MEHPIRERVEDLARRREQALSPGSQRSIDRQHERGKMLARERLE
                     YFLDEGSFHELDMLARHRAGEALGDRPYGDGVITGWGTVDGRKVFVFSQDFTVYGGAL
                     GEVFAEKIMKLMDLATKVGAPVIGLNDGAGARIQEGVVSLASYGGIFYRNVKASGVTP
                     QISVIMGPCAGGAVYSPVMTDFVFMVDETSYMFITGPDVVKQVTGEEVSQQQLGGART
                     HTGTSGVAQFISADDKACLDDVRYLLGFLPSNNLEEAPLEPTADPADRRCEELYDIIP
                     SSANQPYDMRDVVRTVVDDGDFFEYARDWAGSIICGFARLGGTSVGIVGNQPAMYAGV
                     LDIESSSKAARFVRTCDAFNIPLVTFVDVPGFLPGVDQEHNGIIRHGAKLLYAYCEAT
                     VPRISVITRKAYGGAYVVMDSKSIGSDLAFAYPSAELAVMGAAGAVEIVNRRELAEAA
                     DPVARRAELVEAYEETYLNPWEATDRGYVDDVIDPAETRIKLIAALDVLRTKREDLPN
                     RKHGNMPL"
     gene            3195..3785
                     /locus_tag="PLZ72_10275"
     CDS             3195..3785
                     /locus_tag="PLZ72_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA36724.1"
                     /translation="MTAPTLCVYCASRLGEDPTFAEAAAEVGDLMAARGIDLVYGGGQ
                     VGLMGIVADRVMAGGGRVTGVITDHLMKPEVAHHGIDELVVVADMSERKRVMIDRADA
                     FLTLPGGVGTMEEMFEVLCWSYLGLHPKPTGLLAVGTYFDHLIAFLDHSVALGLSKPR
                     ARDLLVVDSDAAALIDTLVPPSGAAGPAPTGEGVEP"
     gene            3788..4867
                     /locus_tag="PLZ72_10280"
     CDS             3788..4867
                     /locus_tag="PLZ72_10280"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNA36725.1"
                     /translation="MRLPVLPPLRPMLAKATKEIPLGEYLYEPKWDGFRCIVFRDGDE
                     IVLGSRNEKPLTRYFPELMEPIRRMLPTRCVLDGELVVPGPDGLVFDLLQQRIHPAES
                     RVRMLSETTPAHLVVFDLIALDDLDLMPLALQERRATLERLLDDVEAPIHLTPATRDP
                     ERATDWFELFDGSGFDGVMAKSLDSAYTPDKRTQLKVKHHRTTDCVVAGYRHHKDGGV
                     GSLLLGLFDDQEPPALHHVGVASAFAASQRRTLAAELAPYEEGGADGHPWRSWAEADA
                     HGDGVRMPGAPSRWSGGKASAEWVPLRCELVVEVTYENLTAGRFRHPARFVRWRPDKD
                     PAQCDYSQLEHPPPIEYTEIFDGPG"
     gene            complement(4904..5782)
                     /locus_tag="PLZ72_10285"
     CDS             complement(4904..5782)
                     /locus_tag="PLZ72_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNA36726.1"
                     /translation="MQVHLVDGTYELFRYFYSPGGGHSNAADEQVGAVRGVLRSLLGM
                     LGDGVTHLGVATDHVIESFRNDLWPGYKDGSGIDPDLLRQFPWVEEAIAALGVTVWPM
                     VEVEADDALGAAAVVAAADDRVERVVICTPDKDLAQCVGGKVVQYDRRAQQYRDSDGV
                     LEKYGVEPASIPDWLALVGDSADGFPGLPGWGAKTAAAVLRRYGRIEDIPHAPGQWDV
                     SGVRGAAKLATTLHDDLDRALLFKTLATLVTDVEVGAVDDWLWAGPTDALADWADRLD
                     APDLVRNADRLAAERL"
     gene            5961..6197
                     /locus_tag="PLZ72_10290"
     CDS             5961..6197
                     /locus_tag="PLZ72_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNA36727.1"
                     /translation="MSEASIRELRNHGGEVVDRVRAGEVVTITRDGEPVAELRPLPRQ
                     PLSAAALVEQFRHLPPLDADRLRADVDAFLDQSL"
     gene            6194..6628
                     /locus_tag="PLZ72_10295"
     CDS             6194..6628
                     /locus_tag="PLZ72_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA36728.1"
                     /translation="MSETTTDPGDGRGVLDTNAVVLLDRLDAADLPDRPTITTVTLAE
                     LSVGPLVTDDDQERAARQARLQAAEASFDPLPLDVAAARTFGRVAAGLRRAGRKPQAR
                     AFDALIAAIAIANELPLYTCNEGDFAGIPELRLRPIPHPDQN"
     gene            complement(6751..7125)
                     /locus_tag="PLZ72_10300"
     CDS             complement(6751..7125)
                     /locus_tag="PLZ72_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36729.1"
                     /translation="MQLAERRFRPEFAAAFDAWFAADPANDPAAPSDPTSMDQYRQPA
                     KERSAALASEAEARFRDGVETGDTADQYVRITVLLASVLFILGISSQFRLRSARVGLI
                     SVGVVILLYAVVLLASAPKPPF"
     gene            complement(7112..7444)
                     /locus_tag="PLZ72_10305"
     CDS             complement(7112..7444)
                     /locus_tag="PLZ72_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36730.1"
                     /translation="MSEGLSASEVGKEIAAHHQHHADRTGEHRVVTIVEAVLLAVVAL
                     LAAWSGYAAAKWSTESRLHLAESASARAKSSQADLEAMETRNLDAMMFDTWFDAWVQG
                     DAQADAAR"
     gene            7548..7949
                     /locus_tag="PLZ72_10310"
     CDS             7548..7949
                     /locus_tag="PLZ72_10310"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA36731.1"
                     /translation="MATIRRDVRFAHPAELVWAAVTDTASLDSWFPGLAACTQEGDVR
                     TVTLGSGLSLPERIVTNDPVQRRLQYRIEAGPVREHLATVDVIDLGDDTCLGVYSTDA
                     EPDVMALVIGAACGDALLELQRQLDAGERAN"
     gene            7976..9544
                     /locus_tag="PLZ72_10315"
     CDS             7976..9544
                     /locus_tag="PLZ72_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNA36732.1"
                     /translation="MGRKILFITTDQQRYDTLGCNGGTLSRTPVIDALASDGIRYERA
                     VPQSVVCMPSRSTMLTGQHPHTHGVWMNGVPLPVDAPSVAGLLHDEGYRTALIGKAHF
                     EPFLDPFLRFNENRLAGLGTETIEDRRFGGGNGPHRGFDHLEFATHGAAGWLHYARWL
                     GQEHPEAAAGYYAVLDRDLEVNADGFGDTGAPQVKHNPIERGWYHTDWVADRTIAWLD
                     GLDVDDDFFCWMSFPDPHHPWDPPESELHRVDWRDVELPAGYPTSAGEREAILDAKPR
                     HWRLWYDGALVSNYEAPARWVPATLTADQVREVNALNAIEVELIDEAVGRVLAAIDAR
                     GWSDDLDVVFTTDHGEFQGDFGLLFKGPYHVDALMRLPLIWRPAPAAGVAPATVTAPV
                     GLVDLAPTFASIAGVDVPDWMQGVELPVDDPDAAERGIEAQLTAWDSELAGVAVHLRT
                     ITRDGWVCTAYLPGAVHDGTEGELYSLVDDPMQRVNRWDDPALRSVRDDLVADLWDRQ
                     PPVRHPRLEVQAPV"
     gene            complement(9633..9935)
                     /locus_tag="PLZ72_10320"
     CDS             complement(9633..9935)
                     /locus_tag="PLZ72_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNA36733.1"
                     /translation="MPGLLRGVARTAAIAGTATAVSGRVQRRQAEKFAQRDANIYAQR
                     EQAYEQQVAPQAAPAPAPAPAGDDKLDQLQKLGDLKAQGILTDAEFEAQKAKILAG"
     gene            complement(9985..10413)
                     /locus_tag="PLZ72_10325"
     CDS             complement(9985..10413)
                     /locus_tag="PLZ72_10325"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HNA36734.1"
                     /translation="MTDTELLPGPIDYVLLEFPGDVPLDGAAAELLALVDSGIVSVWD
                     LVAIRKEADGTFSGIALDAIHESFTAFEGARSGLLGDDDIAEAAAALEPGTVAVLIVW
                     ENAWAERFVTAAFGAGGAVVASGRIPAAEVIEALDLLESE"
     gene            complement(10596..11681)
                     /locus_tag="PLZ72_10330"
     CDS             complement(10596..11681)
                     /locus_tag="PLZ72_10330"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HNA36735.1"
                     /translation="MPHEVRGVVARAVGEPVTIETIVVPDPGPGEALVRVQACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGVVEAVGDGVANVEPGDFVVLNWRAVCGECRACRKG
                     KPWYCFATHNATRKMTLADGTELTPALGIGAFAEKTLVAAGQCTRVDPAAEPEVAGLL
                     GCGVMAGIGAAINTGGVGRGDSVAVFGCGGVGDGAIEGARLAGASTIVAVDVDDRKLE
                     QARRFGATHTVNSTRQDPVEAVRAATGGFGADVVIEAIGLPQTYRQAFEARDLAGTVV
                     LVGVPRPDMTLELPFLEVFGRGGALKSSWYGDCLPERDFPMLIDLYLQDRLNLKGFVS
                     ETISLDDVEEAFHKMERGEVLRSVVVM"
BASE COUNT         1751 a         4300 c         4127 g         1680 t
ORIGIN      
        1 gcgatcgcgc accgggcgag ccacggcacc atggcgcggg cgagtcccgg gtcgcgcagg
       61 taggcgcgcg ccgcggcgac cgtctgcgcc ggcgtcggct cgctccagcg ctggtcgaga
      121 cccgacccgc ccatctcggc ggtgaacagc ccgctggtga acaggcgacg gatcccgtcg
      181 gagccgatcg cgctgctgca ccgccggaac gcgtcgtagg cggccagacc gcctccgtgc
      241 tcggcgtgga acgcgtgctg ataggcccac agcgcctcgt cggaccccgg gtcacggtgg
      301 gcgacgacct gctcggcgag aaccgccccc gccatcagac ccgtcccgat cccgctgccg
      361 tgggccggga acacctggca cgcggcgtca ccgacgaggg cgaccccgcc ggcgccgagc
      421 cgggcgaacg gcctccgcag cgggatgagc ccgtcaccgc cgtagatcgg atcgccgatc
      481 cacgggttct cggcccgggc ccggtccagg atcgtgcggc cgctccccca tgaccccgag
      541 gcgatcgtcc cggtcagcac cgacacccgc tccaggtcgg gttggaccgt caccgccagc
      601 gccgagaacc cgccgtcgag acccacccag gtgacgccgt cgcccgctcg gacgccgcgt
      661 cggtcgagga accgtcgggc gccgtcgacc tcggccacgc gaaacatgaa ctggctggcg
      721 gtgcacaaca ggtcccgggc cacgggcgga caccactgca ccaggtcacg cacctgcgag
      781 cgcagcacgc cgtgtcgacc cgccgcgtcg acgaacaggt cggcctcgag gtgcaacgcc
      841 cgggcacccg cctcgtcacc gtgctgtccg tcggaggggc cgtcgtcggg atcgcagccg
      901 acgagatcca gggcgcggac ccggccgtcc acctccgaga cgaccacgtc gcgggcccgg
      961 tccacgaagg cgacaccctc ggctcggcac agtccgacca gccggtcgtt caggcgtcgc
     1021 atgtcggcat ggcgagtggg cgactgcacc gtcaccggac cggcgtcggc cgggccgtac
     1081 agccgggtca cgccggtggt gtcgtgcacc tcgtccggcg tcggtggggc gatgccggcc
     1141 cgggtgtact gccagtcgag caccccgttg tcccagcggg cgccgccctc accgagtcga
     1201 cgggactcga cgacggtgac ctgcaggcct cgtcgagcca actgcaacgc cgcgttgagt
     1261 cccgctgtcc cggcgccgag caccaccgcg gcggcccctg ccggattccc gtgaccgttc
     1321 gcgccaccag tggcgtcagg agtcatgaga atcggggccg ggggcggtca gccgggccgg
     1381 ccgcgacgga ccggtaccgg cttggaccac caccgtcccg accaccgcca gcgcgacacg
     1441 cgctccggtc cgacctccgc ggatcctccg ggcagcccgc actggatcgc agcgacgatc
     1501 gctgcggcct cctcgtcggt gggctcgggg gtgatgtgca cgctcatgat gtgctcgtcg
     1561 gattcctgcc gctcacagcg gcatgttgcc gtgcttgcgg ttgggcaggt cctcgcgctt
     1621 ggtgcgcagc acgtcgagcg ccgcgatcag cttgatgcgg gtctcggccg gatcgatcac
     1681 gtcgtcgacg tagccgcggt cggtcgcctc ccacgggttg aggtaggtct cctcgtaggc
     1741 ctcgacgagc tcggcccggc gggccaccgg gtcggctgcc tccgcgagct cccgccggtt
     1801 cacgatctcg accgcgcccg cggcacccat caccgccagc tcggcggagg gataggcgaa
     1861 cgccaggtcc gagccgatcg acttggagtc catcaccacg tacgccccgc cgtaggcctt
     1921 gcgggtgatc accgagatgc gcggcaccgt cgcctcgcag tatgcgtaca gcagcttggc
     1981 gccgtggcgg atgatcccgt tgtgctcctg gtcgacgccg ggcaggaacc cggggacgtc
     2041 gacgaaggtg acgagcggga tgttgaacgc gtcgcaggtc cgcacgaagc gagccgcctt
     2101 ggacgacgac tcgatgtcga gcacaccggc gtacatggcc ggctggttgc cgacgatgcc
     2161 gacgctggtg ccacccagtc gggcgaaacc gcagatgatc gatccggccc agtcccgcgc
     2221 gtactcgaag aagtcgccgt cgtcgacgac ggtccgcacc acgtcgcgca tgtcgtaggg
     2281 ctggttggcc gacgacggga tgatgtcgta gagctcctcg cagcgacggt ccgccggatc
     2341 cgcagtcggc tcgaggggtg cctcctcgag gttgttggag ggcaggaagc cgagcaggta
     2401 gcgcacgtcg tcgaggcacg ccttgtcgtc cgcggagatg aactgcgcca cgcccgaggt
     2461 gccggtgtgg gtgcgggcgc cgccgagctg ctgctgggag acctcctcgc cggtcacctg
     2521 cttcaccacg tcggggccgg tgatgaacat gtaggacgtc tcgtcgacca tgaacacgaa
     2581 gtcggtcatg accgggctgt agaccgctcc gccggcgcac gggcccatga tcacgctgat
     2641 ctgcggggtg acaccggacg ccttgacgtt ccggtagaag atgccgccgt agctggccag
     2701 ggagacgacg ccctcctgga tgcgggcccc ggcgccgtcg ttgaggccga tgaccggcgc
     2761 cccgaccttg gtggccaggt ccatcagctt catgatcttc tcggcgaaca cctcaccgag
     2821 ggcgccgccg tagacggtga agtcctggct gaacacgaac accttgcggc cgtcgacggt
     2881 cccccagccg gtgatgacgc cgtcgccgta gggacggtcg ccgagcgcct caccggcgcg
     2941 gtggcgggcg agcatgtcga gctcgtggaa ggagccctcg tcgaggaagt actcgaggcg
     3001 ctcgcgggcg agcatcttgc cccgctcgtg ctgacggtcg atcgaacgct gcgaaccggg
     3061 ggacagcgcc tgctcacgac gccgggcgag gtcctcgacc cgctctcgaa tggggtgctc
     3121 catggatccg aacggtactt gcgccctcgc cccggcccca aaggcgcagc gccggagcgg
     3181 tagcgtcgcc cgccgtgacc gctccgaccc tgtgcgtcta ctgcgcctcc cgcctcgggg
     3241 aggaccccac gttcgcggag gccgccgccg aggtgggcga cctgatggcg gcccgaggca
     3301 tcgacctggt ctacggcggc ggtcaggtcg ggctgatggg gatcgtggcc gaccgggtca
     3361 tggccggtgg aggccgggtc accggcgtga tcacggacca cctgatgaag ccggaggtcg
     3421 cccaccacgg catcgacgag ctggtggtcg tggccgacat gtccgaacgg aagcgggtga
     3481 tgatcgatcg ggccgacgcg ttcctgacgc tcccgggcgg ggtgggcacg atggaggaga
     3541 tgttcgaggt gctgtgctgg tcgtacctcg gactgcaccc caagccgacc ggcctgctcg
     3601 ccgtcgggac ctacttcgac cacctgatcg ccttcctcga ccactcggtg gcactggggc
     3661 tcagcaaacc ccgggcccgg gacctgctcg tggtcgactc cgatgccgcc gccctgatcg
     3721 acacgctcgt cccgccgtcc ggtgccgcgg ggccggcccc caccggagag ggtgtagaac
     3781 cgtaggggtg cggctcccgg tcctcccacc gctgcggccg atgctggcca aggccaccaa
     3841 ggagatccct ctcggggagt acctgtacga gccgaagtgg gacggcttcc gctgcatcgt
     3901 gttccgcgac ggcgacgaga tcgtgctcgg gagccgcaac gagaagccgc tcacgcggta
     3961 cttccccgag ctcatggagc cgatccgccg gatgctgccg acccggtgcg tgctcgacgg
     4021 cgagctggtc gtgccggggc cggacggact ggtcttcgac ctgctccagc agcgcatcca
     4081 ccccgccgag agccgggttc ggatgctgtc cgagaccacg cccgcgcacc tggtcgtgtt
     4141 cgacctgatc gccctcgacg acctggacct catgcccctt gcgctccagg agcgccgagc
     4201 gacgctcgaa cgcctgctcg acgacgtcga ggcaccgatc cacctgaccc cggccacccg
     4261 cgacccggag cgggccaccg actggttcga gctcttcgac ggatccggtt tcgacggcgt
     4321 gatggccaag tcgctcgact cagcctacac ccccgacaag cggacgcagc tcaaggtcaa
     4381 gcaccaccgg accaccgact gcgtcgtggc cggctaccgc caccacaagg acggcggggt
     4441 gggctcgctc ctgctcggcc tgttcgacga ccaggagccg ccggcgctgc accacgtcgg
     4501 ggtcgcgtcc gcgttcgccg cctcccagcg caggaccctg gccgccgagc tcgctcccta
     4561 cgaggagggc ggagccgacg gccacccgtg gcggtcctgg gccgaggccg acgcccacgg
     4621 cgacggggtg cggatgcccg gggcccccag ccggtggtcg ggcgggaagg ccagcgccga
     4681 gtgggtgccg ctgcgctgcg agctggtggt cgaggtcacc tacgagaacc tgaccgcggg
     4741 tcggttccgc cacccggcca ggttcgtgcg gtggcgaccg gacaaggacc cggcgcagtg
     4801 cgactactcc cagctcgagc acccgccgcc gatcgagtac accgagatct tcgacggccc
     4861 cggctgagaa cggcgtcgtc gggcgagacg ccgacgatcg acctcacaga cgctcggccg
     4921 ccaggcggtc ggcgttgcgg accaggtcgg gggcgtcgag ccggtccgcc cagtcggcca
     4981 gggcgtcggt gggacccgcc cagagccagt cgtcgaccgc gccgacctcg acgtcggtga
     5041 ccagggtggc gagcgtcttg aacagcagcg cccgatcgag atcgtcgtgc aacgtcgtcg
     5101 cgagcttggc cgcacctcgg acgcccgaca cgtcccactg gccgggggcg tgggggatgt
     5161 cctcgatccg gccgtagcgc cgcaggaccg ccgcggcggt cttggccccc cagcccggca
     5221 ggcccgggaa gccgtcggcg ctgtcgccga cgagcgcgag ccagtcgggg atggaggcgg
     5281 gttcgacgcc gtacttctcg agcacgccgt cggagtcgcg gtactgctgg gcgcgtcggt
     5341 cgtactggac gaccttgccg ccgacgcact gggccaggtc cttgtccggc gtgcagatga
     5401 ccacccgctc gacgcggtcg tccgccgcgg cgacgacggc cgcggccccg agggcgtcgt
     5461 cggcctccac ctcgaccatg ggccacaccg tcacgccgag tgcggcgatc gcctcctcga
     5521 cccaggggaa ctgccgcaac aggtcggggt cgatccccga tccgtccttg tagcccggcc
     5581 acaggtcgtt gcggaacgac tcgatgacgt ggtcggtggc cacgccgagg tgggtgaccc
     5641 cgtcgccgag catgccgagg agcgatcgga gcacgccgcg caccgctccg acctgctcgt
     5701 cggcagcgtt cgagtgacca ccacccggtg agtagaagta ccggaacagc tcgtaggtgc
     5761 cgtcgacgag gtggacctgc atgctgcttc ccctactggt agcgcggttc tggcgtcttc
     5821 gatggcagaa ccaacgcaac acactgacac agcagcgatc acagcatccc ccgccgtggc
     5881 ggacgactct gcgtcgtgtg gtccgacagc gaggcggatc cgaccttcga tactcgtctc
     5941 acgggtgata cactcgttgc atgtccgagg ccagcatcag agagctccgc aaccacggcg
     6001 gcgaggtggt cgaccgcgtg cgagccggtg aggtggtcac catcacccgg gacggcgagc
     6061 cggtcgccga gctacggcca ctgccccgcc agccgttgag tgccgctgca ctggtcgagc
     6121 agttccggca cctgccaccc ctcgacgccg accgcctccg cgccgacgtc gacgccttcc
     6181 tcgaccagtc gctgtgagcg agaccacgac ggacccgggc gacgggcggg gtgtgctcga
     6241 caccaacgcc gtggtgctgc tcgaccgact cgatgccgct gaccttcccg accggccgac
     6301 catcaccacc gtcaccctgg cggagctgtc ggtcggaccg ctcgtcaccg acgacgacca
     6361 ggagcgtgcc gcccgacagg cacggctcca agcagccgag gcgtcgttcg atccgctgcc
     6421 gctcgacgtt gccgccgccc ggacgttcgg gcgggtggca gccgggctgc gccgagccgg
     6481 acgcaagccg caggcccggg ccttcgacgc cctgatcgcc gccatcgcca tcgccaacga
     6541 gctgccgctg tacacctgca acgagggcga cttcgccggg atcccggagc tacgactccg
     6601 cccgatcccg catcccgatc agaactgacc cacctccggc tgacacaact ttcctggcac
     6661 accgcccccg atcggagcgg gggcgggtgg cacccgagaa tccgctcacc ggcgcaccgg
     6721 tccgacaccc ggcccgcagg aggacgacgc tcagaaggga ggcttcggag ccgacgccag
     6781 gagcacgacc gcgtacagca ggatcacgac accgaccgag atcaagccga cgcgggccga
     6841 gcgcagacgg aactggctgc tgatgcccag gatgaacagc accgacgcca gcagaacggt
     6901 gatgcgcacg tattgatcgg cggtgtcgcc cgtctcgaca ccgtcgcgga acctggcctc
     6961 ggcctcggag gccagagccg cgctgcgctc cttggccggc tggcggtact gatccatcga
     7021 cgtcggatcc gagggtgcgg cggggtcgtt cgctggatcg gcggcgaacc acgcgtcgaa
     7081 ggccgccgcg aactcgggcc ggaatcggcg ttcagcgagc tgcatcggcc tgagcgtccc
     7141 cctggaccca ggcgtcgaac caggtgtcga acatcatcgc gtcgaggttc cgggtctcca
     7201 tcgcctcgag gtcggcctgg ctggacttcg cgcgggctga tgcggattcg gccaggtgca
     7261 gccgggactc cgtgctccac ttcgcggcgg cgtaccccga ccacgccgcg agcagagcga
     7321 cgaccgccag gaggaccgcc tcgacgatgg tgaccacgcg gtgctcaccg gtccggtccg
     7381 cgtggtgctg gtgatgggcc gcgatctcct tgccgacctc gctcgccgac agaccttcgc
     7441 tcacgtgcac cctccggacg tgtcgagcgg ccgggggatc ggatcggccg ggctacatga
     7501 tgcccgcgcc gccactgccg acgcggagct ggcatgatcg gccaccgatg gccacgatcc
     7561 gacgcgacgt ccgattcgcc caccctgcgg agctggtgtg ggcggccgtg accgacaccg
     7621 cctcgctcga cagctggttc cccggcctcg ccgcatgcac ccaggagggc gacgtccgca
     7681 ccgtcacgct cgggtcggga ctgagcctgc ccgagcgcat cgtcaccaac gacccggtgc
     7741 agcggcggct ccagtaccgc atcgaagccg ggccggtccg cgagcacctc gccaccgtcg
     7801 acgtcatcga cctgggcgac gacacctgcc tgggcgtgta ctccaccgac gcggagcccg
     7861 acgtgatggc gctcgtcatc ggcgccgcgt gcggcgacgc cctgctcgag ctgcaacgcc
     7921 agctcgacgc cggcgaacga gccaactgac ggcacgatcg agcacaggaa gcgcgatggg
     7981 acgcaagatc ctcttcatca ccaccgacca gcagcgctac gacacgctcg gctgcaacgg
     8041 cgggaccctc agccggacac cggtcatcga cgctctcgcg tccgacggca tccgctacga
     8101 gcgggccgtg ccgcagtcgg tggtgtgcat gccgtcgcgc tcgacgatgc tcacggggca
     8161 gcacccccac acccacggcg tgtggatgaa cggggtgccc ctcccggtcg acgcgccgtc
     8221 ggtcgccggg ttgctccacg acgaggggta ccgcacggcg ctgatcggca aggcgcactt
     8281 cgaaccgttc ctcgacccgt tcctgcgctt caacgagaac cggctggccg gactcggcac
     8341 cgagacgatc gaggatcgtc gcttcggcgg tggcaacggt ccgcaccggg gcttcgacca
     8401 cctcgagttc gcgacccatg gcgcggccgg gtggttgcac tacgcccggt ggctcggcca
     8461 ggagcacccc gaggcggccg ccggctacta cgcggtgctc gaccgcgacc tcgaggtgaa
     8521 cgccgacggc ttcggagaca ccggcgcgcc ccaggtgaag cacaacccga tcgagcgcgg
     8581 ttggtaccac accgactggg tggcggaccg gacgatcgcc tggctcgacg gcctcgacgt
     8641 cgacgacgac ttcttctgct ggatgagctt cccggacccg caccacccgt gggacccgcc
     8701 cgagtcggag ctgcaccgcg tcgactggcg cgacgtcgag ctcccggccg gctatccgac
     8761 ctctgccggt gagcgcgagg cgatcctcga cgccaagccc cggcactggc ggctctggta
     8821 cgacggggcg ttggtgtcga actacgaggc cccggcccgc tgggttcccg ccaccctgac
     8881 cgccgatcag gtccgcgagg tcaacgcgct gaacgcgatc gaggtcgagt tgatcgacga
     8941 ggcggtcggc cgggtcctcg cggcgatcga cgcccgcggc tggtccgacg acctcgacgt
     9001 cgtgttcacc accgaccacg gcgagttcca gggcgacttc ggcctgttgt tcaagggccc
     9061 gtaccacgtc gacgccctga tgcgtctgcc gttgatctgg cgaccggctc cggcggcggg
     9121 cgtcgcaccg gcgacggtga ccgcgccggt gggcctcgtc gacctggccc cgacgttcgc
     9181 gtcgatcgcc ggggtggacg tgccggactg gatgcagggg gtggagctgc ccgtcgacga
     9241 cccggacgcc gccgaacgcg ggatcgaggc ccagctcacc gcgtgggaca gcgagctggc
     9301 cggcgtggcg gtgcacctgc gaacgatcac ccgcgacggg tgggtgtgca cggcgtacct
     9361 gcccggtgcg gtgcacgacg gcaccgaagg ggagctgtac tcgctggtcg acgacccgat
     9421 gcagcgcgtc aaccgctggg acgacccggc gctgcgctcg gtccgcgacg acctcgtcgc
     9481 cgacctgtgg gatcgtcagc ctccggtccg acatccccgc ctcgaggtcc aggctccggt
     9541 ctgaacagct tctgaacgtt cgctggacgc gcaactggct gccgttcccg cctcgggcgg
     9601 tgacgacagc cagtcggagt gcgggtggct cgtcagccgg cgaggatctt ggccttctgg
     9661 gcctcgaact cggcgtcggt gaggatgccc tgcgccttca ggtcgccgag cttctggagc
     9721 tggtcgagct tgtcgtcgcc cgccggtgcg ggagccggag ccggtgcggc ctgcggggcg
     9781 acctgctgct cgtaggcctg ttcgcgctgg gcgtagatgt tggcgtcgcg ctgggcgaac
     9841 ttctcggcct gtcgacgctg cacccggccg ctgacggcgg tggcggtgcc ggcgatagcg
     9901 gcggttcggg cgacgcccct cagcagtcct ggcatggtgg ttctccttga agtcgatcgg
     9961 atcggtgggt gtcggggttc acggctactc ggactcgagc aggtcgagcg cctcgatgac
    10021 ctcggccgcg gggatgcgcc cggacgcgac caccgcgccc ccggcgccga aggcggcggt
    10081 gacgaaccgc tccgcccacg cgttctccca gacgatcagc accgccaccg tcccgggttc
    10141 gagagcggcg gccgcctcgg cgatgtcgtc gtcgccgaga agtccgctgc gggcgccctc
    10201 gaacgccgtg aacgactcgt ggatcgcgtc gagtgcgatc ccggagaagg tgccgtcggc
    10261 ctctttgcgg atggcgacca ggtcccagac cgacacgatc ccgctgtcga cgagggcgag
    10321 cagctcggcc gcggctccgt cgagcgggac gtcgccgggg aactcgagga ggacgtagtc
    10381 gatcggtccc gggagcagtt cggtgtcggt catcggttca ggcttctccg tgaaggtgtg
    10441 tcgtgatcag gtgatgagcg ccgacggcga tcatcacggg cacgagcgag ctcgtgtggg
    10501 ctgaacggtt ggagcgattg tgctcgaccc gacccgccgc tgcatcaccc ggatcgggcg
    10561 aaccgacgtt acccgggccg gcgccacgga accgctcaca tcacgacgac cgaacgcagc
    10621 acctcgccac gctccatctt gtggaacgcc tcctcgacgt cgtcgagcga gatcgtctcg
    10681 gacacgaacc ccttcaggtt caagcggtcc tgcaggtaga ggtcgatcaa catggggaag
    10741 tcccgctcgg gcaggcagtc gccgtaccac gacgacttga gggcgccgcc ccggccgaac
    10801 acctcgagga agggcagctc cagggtcatg tcgggtcggg gcaccccgac gagcacgacg
    10861 gtgccggcca ggtcccgggc ctcgaacgcc tggcggtacg tctgcggcag gccgatcgcc
    10921 tcgatcacca catccgcgcc gaacccgccg gtggcggccc ggacggcctc gacggggtcc
    10981 tgccgggtgg agttgacggt gtgggtggcg ccgaaccgtc gggcctgctc gagcttgcgg
    11041 tcgtccacgt cgaccgccac gatcgtcgac gcccccgcca gacgagcccc ctcgatggcg
    11101 ccgtcgccca cgccgccgca gccgaacacc gcgaccgagt cgccccgacc gacgccgccg
    11161 gtgttgatcg cggcgccgat gccggccatg accccgcagc cgagcaggcc ggcgacctcg
    11221 ggctcggcgg ccgggtcgac cctggtgcac tggccggcgg cgacgagcgt cttctccgcg
    11281 aatgccccga tcccgagcgc cggggtcaac tcggtgccgt cggcgagcgt catcttccgg
    11341 gtggcgttgt gggtggcgaa gcagtaccac ggcttgccct tccggcacgc ccgacactcc
    11401 ccgcagaccg ctcgccagtt gaggacgacg aagtcgccgg gttccacgtt ggcgacgccg
    11461 tcaccgaccg cctcgacgac gccggcggcc tcgtggccca gcaggaacgg gaactcgtcg
    11521 ttgatcccac cttcccggta gtggaggtcg gtgtggcaca ccccgcacgc ctggacccgc
    11581 accagggctt cgcccggacc ggggtcgggc acgacgatcg tctcgatcgt gaccggctct
    11641 ccgaccgctc gggccaccac tcctcggacc tcgtgtggca tcggttgtct ccccctccgt
    11701 ggagccgcac cccggcggtg acggcggcgc ctcgtggcgc atcgccatcc cagcacaacg
    11761 cccggtagcc cgctcgggat cgaggggccg cgtcatcgcg acccggaact gcggccagtc
    11821 cccaacacct cgggacatgc aatatcgcat gagacaat
//
LOCUS       DAOQOD010000098        11808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1930_length_11808_cov_11.895346, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000098 DAOQOD010000000
VERSION     DAOQOD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1930_length_11808_cov_11.895346"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1255)
                     /locus_tag="PLZ72_10335"
     CDS             complement(8..1255)
                     /locus_tag="PLZ72_10335"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNA36736.1"
                     /translation="MPVTPIPELARRAKAASRRLATASTALKDEALLTAADLLEARSA
                     DVLAANSLDVAGAEGAGIPTGLVDRLRLTEDRVAAMASGLRQVAALADPVGEVLDGWV
                     RPNGLRISRVRVPLGVVAIIYESRPNVTSDAAGLCLKSGNAAFLRGSSTAVNSNLAIA
                     AVLREAVAKAGLPEDSVIVVEDTSRDAAVEFMQQEGLVDCLIPRGGASLIASIKQHAT
                     VPFVIDGDGNCHVYVDAAADLDMAETIVLNAKTQRPSVCNAAESLVVHAQVAAQILPR
                     LDTALADVELVGDARARALVPRMGEATDEDYAREFLDMRMSVRVVDTLDEAIEHVNRH
                     STGHSEAIVTSDVGAAERFLNEVDAAAVLLNASTRFVDGEEFGFGAEIGISTQKLHAR
                     GPMGLQQLTTAKYVVRGDGQTRG"
     gene            1286..>2661
                     /locus_tag="PLZ72_10340"
     CDS             1286..>2661
                     /locus_tag="PLZ72_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA36737.1"
                     /translation="MGAGPLDAVVSAFIADVGDALADAAGDLDGVDPDRFHDDVTVEA
                     FNLTVAMIDADQRHTDEELDALIDAFGPRLTDSQLIHAAPETLRGSSLVADHRRWLEV
                     DSELFTILVESDSRRGTTGADRYYERALDIAHVVASLDVIPAREELEAISTLRTRLLD
                     GLRRRDPDRPTVAVAGPTPSGEAGPTVVDPPPRPLEELLAELDELVGLTEVKARVRLV
                     ADFLRVQQLRAERDLPTVETSHHLVFTGNPGTGKTTVARLLAQIYRTLGVVARGHLVE
                     TDRSGLVAGYVGQTAPLVTRRFDEADEGMLFIDEAYTLVRGGENDFGREAIDQIVKLM
                     EDRRDRIVVVVAGYTDEMDQLLSSNPGLRSRFPTVIVFPDYSDDELVAIVDSIGERQR
                     YELTDDARTKLRSVLAATPRGHGFGNARLARNLFEAAINNHARRIMADSDAPTEADLT
                     TLVAEDLP"
     assembly_gap    2662..2761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2762..3743
                     /locus_tag="PLZ72_10345"
     CDS             <2762..3743
                     /locus_tag="PLZ72_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36738.1"
                     /translation="AAWWVRTSFIDDDSGGGNAGGERLDLLCGSELADVCDRLAAEDD
                     SLRVTVVDDGTTTDDLSVDGATAGFDAWLSAGPWPAIVADNRAAAGLRTDPLGGTSRV
                     LGRSPVTLVGPIDRVEALEQHCGGTISWSCVGDVSGQAWSSLGGSPAWGAFRTGLTPV
                     DTGGGLVALDQAVANRSGTTEWSTTELDDVSDWLSQLVGAATFSNDPLDVMLTRPGSF
                     SLTAPLERRSGPAFGRADTGNRFRLLYPEPMVTADVTLSAAAGEDVDDLLDRLGPERV
                     ARALTDGGWRLPGQRSPFTVGGSPELPTESGLTTPGALQALRTTVQEMRP"
     gene            3740..>5505
                     /locus_tag="PLZ72_10350"
     CDS             3740..>5505
                     /locus_tag="PLZ72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding and VWA domain-containing
                     protein"
                     /protein_id="HNA36739.1"
                     /translation="MTTRHRSSRVGAAVAAIAAAAVLAAACSGGESGSGDDRSECIQV
                     DAAVSSEKIDLLGDLADDFNDSGATVDGTCIDVEVQKVASGVAAERLADGWDTATDGP
                     RPVIWSPASSAWGQILNQRLSTAGKPAMATDPVSFMNTPLVIAMPRPMAEALGWPDQP
                     IGWSDILALSRAENGWADKGHPEWGRFRLGKTNPNYSTSGLHALIAQTYAATGKTAGL
                     SSEDLQNPTVQQYGRDIESSVVHYGDITMTFLNNWFRADRRGTALNYASAVAVEEKSL
                     IDYNAGNPDGELDQGEKPRKPRTPLVAIYPTEGTLYSDNPLFILDADWVSGPQRAAAQ
                     QFQKFVMEPKNQEKVLKFGFRPGNPDVAVGAPITADNGVDPNQPQTLLEVPSGAVMDE
                     LLGRWAEQRKGARVMLVIDVSGSMGDPADPSDPGGPTKLDLAKEAVIDGLDSFKAEDL
                     VGVRIFTSGIDGGSADFVDLAPVQAIGPNRERIKNQVDQLSPLNGTPLYSVTQRSYDQ
                     MLTGYDPALINAVILLTDGRNDDGNAADDRDQLQSLLADVKNSNDGENSRPVRIFTIA
                     YGGDTNPAELKRISEASNATAYT"
     assembly_gap    5506..5605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5606..6613)
                     /locus_tag="PLZ72_10355"
     CDS             complement(5606..6613)
                     /locus_tag="PLZ72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF020919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HNA36740.1"
                     /translation="MGDGDDLGRGGPAVAAALLAVLALIAAVAVGHGSFGSTDALADG
                     APAHDMAGHRSMPLDDPPVWADGTVATPTRHTGPQGGTGQFVAKCTYSHSGEVDPIVY
                     PGEVGVSHRHDFYGSTNVDENSTPDSMRAGGTTCDKPGDDAAYWQPTLYDGDAVVEPL
                     LIHAYYRAAPGIDPRAVKRFPDGLMMLAGDPFATEAPSTESAGWTCGVRTDLLSEPPN
                     CPVTAPLAMVLTFPDCWDGQNADVPGHRRHMTYSRDGACPEDHPVSVPQLTVSIKYPI
                     SGPGHDLRLASGSVHTAHGDFFNGWVPAALEREIRSCIRRGVVCDLASNRSEEDLFQA
                     S"
     gene            complement(6606..8078)
                     /locus_tag="PLZ72_10360"
     CDS             complement(6606..8078)
                     /locus_tag="PLZ72_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNA36741.1"
                     /translation="MGRRRLVLVAAIAAVALFAGACSSDDSGDGGDDDAKTRVTSVED
                     GLEAIRVPGDQKTIQAAVDSAEAGDLILVSEGVYKEAVDVTTADITIRGVDRNKVILD
                     GEFTADNGIRVLDTDGVVVENMTARNYISNGFYWTGSDRYRGSYLTAYRNGDYGIYAF
                     DSYHGQFDHSYGSGSPDAGFYIGECYKCDAVLSEVVSEYNGLGYSGTNSGGDLYIVNS
                     EFRNNRAGIVPNSGSYELCYPSRGTTIAGNLVHDNNMTDGPGIDVSLLAQGNGILPAG
                     AVRVEITRNRVWNHDRTGIGLVPFPESDANDLAPPQTEWDQPCSESPAKKASESGAKT
                     TIAPDKCVEVELIGDGCIVMWNPMEIKVEGNDVSGSKVADLAVGTVDLTESGQTTDKL
                     GNCFSDNVFTTSQPAQIEQLAPCNGEPTATDWNASALDLIGLMGSPAPKPPADSYKAT
                     PEPPEQENMPKATTAKPVKFTKPMTVDLASIKLPAAPTGG"
     gene            8179..8589
                     /locus_tag="PLZ72_10365"
     CDS             8179..8589
                     /locus_tag="PLZ72_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNA36742.1"
                     /translation="MTSRGIRSIRLALIAGVGLLAVACGGGSSSSFSLPDGATWTKES
                     GNAVTVDTRDNVFVPQFIEVKKGAEVTWDNTGRNRHNVLPDEEGDFKEVQVDELDPGM
                     KASRTFDEVGEFGYYCSLHGTATKGMFGAIKVVE"
     gene            8690..9433
                     /locus_tag="PLZ72_10370"
     CDS             8690..9433
                     /locus_tag="PLZ72_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36743.1"
                     /translation="MSFRDRFFTPRVARATTSPSAILATGGGAAAGMLVGGPIGALIG
                     GLGAYAVRVLAAVPRAPKHPGTDIRGLVDPWRGLMVGVIDARRRFDRAVSSVRPGPLR
                     DRLGEVGTRLDTAVDEAGRIARAGNLLTSGRKQIDVPAIRQDLDLARSAPPTDRTAAT
                     IRAVEAQLESAGRLDRTIADTVDQLRLLDARLDETVTRTVELAATQADSSDLGGIGPE
                     VESIVSELESLRQAVEETGGPTPAPGTTP"
     gene            9430..11139
                     /gene="murJ"
                     /locus_tag="PLZ72_10375"
     CDS             9430..11139
                     /gene="murJ"
                     /locus_tag="PLZ72_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNA36744.1"
                     /translation="MTHDDDLAGHDDLAGHDDLALAGLEDDVALAEAEAGDDVIVDRR
                     RGRSLLAASAMPAVGTTLSRATGLIRVGALTYALGLTTIADVYNLANTTPNILYELVL
                     GGVLSSTLVPLFVRTLDDADDDTASVVTTVAFSAIVAMTLLGVVLAPWINRLFALPLE
                     GADRAQQLRLGDDFLELLIPQILFYGVTTLITALLHARRRFAPPAFTPILTNLVTAAA
                     AVAAAKWFARSGAEMGQVYLLGLGTTAGVAAMAIALVPYIRGSGIQLRWRFQPRHPAV
                     RSVMRLSGWTVGFAAANQVAVLLILTMARAQEAGTVSAYQYAFIFFQLPHGLIAVSLM
                     TAVLPELAEAAVDRDDRAYIARFREGTSLLLTFLLPAAVGLLFVGEPLIRLFLQRGSF
                     TAEDTERTTRMLLGFAIGLPAFSVYLYCVRAFFARRDTRTPFFLNVGQNALNVALVIP
                     LTRLWGAEGLSVAYSASYWVIAVVTLYVLNRRVPHLLTARAMWPFLRSIGVGVAVFVA
                     LGATSLVLGDQVGPVLELLVEVGVAVAVFVPVTFMLKPKGFEPAIDRLRQGMRRRGRS
                     MAG"
     gene            11150..>11808
                     /locus_tag="PLZ72_10380"
     CDS             11150..>11808
                     /locus_tag="PLZ72_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNA36745.1"
                     /translation="MWKAIKRWWAYLGAKVNSVFNEKADPKVQLEQAITEAQEQHRRL
                     KEQAANVIANQKQNQMRLDAALEELERLNRNAQQAVLMADEATRKGDAAKTAEYTSAA
                     EAFANQLIAKEKEVENLKQMSLQATQAAEQAKAAVQQNSRVLQQKLAEKQKLLGQLDQ
                     AKMQEQMNKAMASLSEQVGEDVPTLDEVREKIETRYARAKGMAELQDMSVESKMLEVE
                     QA"
BASE COUNT         1726 a         4142 c         4045 g         1695 t
ORIGIN      
        1 ccactgctca gccccgggtc tggccgtcgc cgcggacgac gtacttggcg gtggtgagct
       61 gctgcaggcc catcggaccg cgggcgtgca gcttctgggt cgagatgccg atctcggcac
      121 cgaagccgaa ctcctcgccg tcgacgaacc gggtcgaggc gttgagcagg accgcggcgg
      181 cgtcgacctc gttcaggaac cgctccgcgg cgccgacgtc agaggtgacg atcgcctcgg
      241 agtgcccggt cgagtgacgg ttcacgtgct cgatcgcctc gtcgagggtg tcgacgacgc
      301 ggaccgacat ccgcatgtcg aggaactcgc gggcgtagtc ctcgtcggtg gcctcaccca
      361 tccgcggcac cagcgcccgg gcccgggcgt caccgaccag ttccacgtcg gccagggccg
      421 tgtcgagccg gggaaggatc tgcgccgcga cctgggcgtg gacgaccagc gactccgccg
      481 cgttgcacac cgagggacgc tgcgtcttgg cgttgagcac gatggtctcg gccatgtcga
      541 ggtccgcggc ggcgtcgacg tagacgtggc agttgccgtc gccgtcgatc acgaacggga
      601 cggtcgcgtg ctgcttgatc gacgcgatga gcgacgcgcc accccggggg atcaggcagt
      661 cgaccagtcc ctcctgctgc atgaactcga cggcggcgtc ccgggaggtg tcctcgacga
      721 cgatgaccga gtcctcgggc agcccggcct tggcgaccgc ctcgcgcagg accgcggcga
      781 tggccaggtt ggagttcacc gccgtcgacg acccccgcag gaacgcggcg ttgccggact
      841 tgaggcacag cccggcagcg tcggaggtca cgttcggacg gctctcgtag atgatcgcca
      901 cgacccccag cggaacccgg acccggctga tccgcagacc gttgggccgc acccagccgt
      961 cgagcacctc gccgaccgga tcggcgaggg cggccacctg acgcagaccc gacgccattg
     1021 cggcgacccg gtcctcggtc agacgcaacc ggtcgacgag tccggtcgga atgcccgccc
     1081 cctcggcccc ggcgacgtcg agcgagttgg cggcgagcac gtcggcggag cgggcctcga
     1141 gcagatccgc cgcggtcagc aacgcctcgt ccttgagcgc ggtggaggcg gtggccagcc
     1201 ggcgcgaggc cgccttggca cgacgggcca gctcggggat gggtgtgacg ggcattcccg
     1261 aaggctaccg atagcctcca ccgccgtggg agccggaccc ctcgacgccg tcgtcagcgc
     1321 cttcatcgcc gacgtcggcg atgccctcgc cgacgcggcg ggcgacctcg acggggtcga
     1381 cccggaccgg ttccacgacg acgtcaccgt cgaggcgttc aacctgaccg tcgccatgat
     1441 cgacgccgac cagcgccaca ccgacgagga gctcgacgcc ctcatcgacg cgttcggtcc
     1501 ccggttgacc gactcgcagc tgatccacgc cgcccccgag acgctgcgcg gctcgtcgct
     1561 ggtggccgat caccgccggt ggctggaggt cgactccgag ctgttcacga tcctggtcga
     1621 gtccgacagc cgtcgcggca ccacgggcgc ggaccgctac tacgaacggg ccctcgacat
     1681 cgcccacgtg gtcgccagcc tggacgtcat cccggcccgg gaggagctcg aagccatctc
     1741 gacgctgcga acccgcctcc tcgacggcct gcgccgacgc gaccccgacc gtccgaccgt
     1801 cgcggtcgcc ggacccacgc cgagcgggga agccggaccg acggtcgtgg atccaccgcc
     1861 ccggcccctc gaggagctgc tcgccgagct cgacgaactg gtcggcctca ccgaggtcaa
     1921 ggcccgggtc cggttggtgg cggacttcct gcgggtccag cagctgcgcg ccgagcggga
     1981 cctccccacc gtggagacct cgcaccacct ggtgttcacc ggcaaccccg gcaccggcaa
     2041 gacgaccgtc gcccgcctgc tggcccagat ctacagaacg ctcggggtcg tggcccgggg
     2101 ccacctggtc gagaccgacc gatcgggtct ggtcgccggt tacgtgggcc agaccgctcc
     2161 gctggtcacc cgacgcttcg acgaggccga cgaggggatg ctgttcatcg acgaggccta
     2221 caccctggtc cgcggtggcg agaacgactt cggtcgcgag gcgatcgacc agatcgtgaa
     2281 gctgatggag gaccggcgcg accgcatcgt ggtcgtggtc gccggataca ccgacgagat
     2341 ggaccagctg ctgtcgtcca acccggggct gcggtcgcgc ttccccaccg tcatcgtctt
     2401 ccccgactac tcggacgacg agctggtcgc catcgtcgac tcgatcgggg aacgccagcg
     2461 ctacgagctg accgacgatg cccggaccaa gctgcgcagc gtgctggccg ccactccgag
     2521 gggacacggc ttcggcaacg cgcgcctggc ccgcaacctg ttcgaggcgg cgatcaacaa
     2581 ccacgcccga cgcatcatgg ccgacagcga cgcgccgacc gaggccgacc tcaccaccct
     2641 ggtggccgag gacctccccg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 ncgcggcgtg gtgggtccgc acatcgttca tcgacgacga ctccggagga gggaacgccg
     2821 ggggcgagcg cctggacctg ctctgtggat cggagctggc cgacgtgtgc gaccgcctgg
     2881 ccgccgagga cgactcgttg cgcgtcaccg tcgtcgacga cggcacgacc accgacgacc
     2941 tgtccgtcga cggcgccacc gcaggcttcg acgcctggct gtccgctggt ccgtggccgg
     3001 cgatcgtcgc cgacaaccgg gccgccgccg ggctgaggac cgaccccctc ggcggaacca
     3061 gccgcgtgct gggccggtcc ccggtcaccc tcgtcggccc catcgaccgc gtcgaggcgc
     3121 tcgagcagca ctgcggcggg acgatcagct ggtcctgcgt cggcgacgtg tccggccagg
     3181 cctggtcgtc gctcggcggg tcgccggcct ggggtgcgtt ccggacggga ctcacccccg
     3241 tcgacaccgg cggcggactc gtcgccctcg accaggccgt cgcgaatcgc agcggaacca
     3301 ccgagtggag caccaccgag ctggacgacg tctcggactg gctgtcccag ctggtcggcg
     3361 ccgccacctt ctcgaacgat cccctcgacg tcatgctcac ccgcccgggg tcgttcagcc
     3421 tgaccgcacc gctggagcgc cgttcgggcc ccgcgttcgg gcgagccgac accggaaacc
     3481 gcttccgcct cctctaccct gaaccgatgg tgaccgctga tgtcacgctg agcgcggcgg
     3541 ccggagaaga cgtcgacgac ctgctggatc gactcggccc cgaacgggtc gcccgggccc
     3601 tgaccgacgg cggctggcgg ctccccggac agcgttcgcc gttcaccgtc ggggggagtc
     3661 ccgagctgcc gacggagtcc gggctgacca ccccgggagc gctccaggcg ctgcggacga
     3721 ccgtgcagga gatgagaccg tgaccacccg acatcgaagc tcccgcgtcg gggccgccgt
     3781 cgctgcgatc gcggccgcgg cggtgctggc ggcggcctgc tccggcggcg agtcagggtc
     3841 cggcgacgac cgcagcgagt gcatccaggt cgacgccgcg gtcagctccg agaagatcga
     3901 cctgctggga gacctggccg acgacttcaa cgactcgggc gcgaccgtcg acggcacgtg
     3961 catcgacgtc gaggtgcaga aggtggcctc cggcgtcgcc gccgagcgcc tggccgacgg
     4021 ctgggacacc gcgaccgacg ggccccgacc ggtgatctgg tccccggcgt cgagcgcctg
     4081 gggtcagatc ctgaaccagc gcctcagcac ggccggcaag ccggcgatgg ccaccgatcc
     4141 ggtgtcgttc atgaacacac ccctggtgat cgccatgccc cgaccgatgg ccgaggcgct
     4201 cggctggccc gaccagccga tcgggtggtc cgacatcctg gcgctgtcgc gggcggagaa
     4261 cggctgggcc gacaagggcc acccggagtg gggccggttc cggctcggca agaccaatcc
     4321 caactactcg acctccgggc tccacgcgct gatcgcccag acctacgccg cgaccggcaa
     4381 gacggccggg ctcagctccg aggacctgca gaacccgacg gtccagcagt acgggcggga
     4441 catcgagagc tcggtcgtgc actacggcga catcaccatg acgttcctca acaactggtt
     4501 ccgggccgac cggcgcggaa ccgccctcaa ctacgcgtcc gcggtcgcgg tcgaggagaa
     4561 gtcgctcatc gactacaacg ccggcaaccc cgacggtgag ctcgaccagg gcgagaagcc
     4621 ccgcaagcca cgcaccccgc tggtggcgat ctacccgacc gaggggacgc tctactccga
     4681 caacccgctg ttcatcctcg acgccgactg ggtcagcggt ccgcagcgcg ccgccgccca
     4741 gcagttccag aagttcgtga tggaaccgaa gaaccaggag aaggtgctga agttcgggtt
     4801 ccggcccggc aaccccgacg tggcggtcgg cgcgccgatc accgccgaca acggcgtcga
     4861 cccgaaccaa ccccagacgc tgctcgaggt cccgtccggc gcggtgatgg acgagctgct
     4921 cggacgctgg gccgagcagc gcaagggggc ccgggtcatg ttggtcatcg acgtgtccgg
     4981 ctcgatgggc gaccccgccg acccgtcgga tccgggtggc ccgaccaagc tggacctggc
     5041 caaggaggcg gtgatcgacg ggctggacag cttcaaggcc gaggacctgg tcggggtgcg
     5101 catcttcacc agcggaatcg atgggggttc ggccgacttc gtcgacctgg cgccggtgca
     5161 ggcgatcggc ccgaaccgcg agcggatcaa gaaccaggtc gaccagctgt cgccgctcaa
     5221 cggcaccccg ctctactcgg tgacccagcg gtcctacgac cagatgctca ccggctacga
     5281 cccggcgctg atcaacgcgg tgatcctgct gaccgacggc cgcaacgacg acggcaatgc
     5341 cgccgatgac cgcgaccagc tgcagtcgtt gctggccgac gtgaagaaca gcaacgacgg
     5401 cgagaactcc cggccggtgc ggatcttcac gatcgcctac ggcggcgaca ccaaccccgc
     5461 cgagctgaag cgcatctccg aggcgtccaa cgccaccgcc tacacnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnntcagg acgcctggaa caggtcttcc tcgctgcggt
     5641 tcgacgccag gtcgcacacc acgccgcggc ggatgcagga gcggatctcc cgctccaggg
     5701 cggccggcac ccagccgttg aagaagtcgc cgtgggcggt gtgcaccgat cccgacgcca
     5761 gccgcagatc gtggccgggg ccgctgatcg ggtacttgat gctgacggtg agctgcggca
     5821 ccgacaccgg gtggtcctcc gggcaggctc cgtcacggga gtaggtcatg tgccgacggt
     5881 ggccgggcac gtccgcgttc tggccgtccc agcagtccgg gaacgtcagc accatcgcca
     5941 acggtgcggt gaccgggcag ttcggcggct ccgacagcag gtcggtgcgc accccgcagg
     6001 tccacccggc cgactcggtg ctcggagcct cggtggcgaa cgggtcgccg gccagcatca
     6061 tgaggccgtc ggggaagcgc ttcaccgctc tcgggtcgat gccgggcgcg gcccggtagt
     6121 aggcgtggat cagcagcggc tccaccacgg cgtcgccgtc gtagagggtc ggctgccagt
     6181 aggcggcgtc gtcgccgggc ttgtcgcagg tggtgccccc ggcgcgcatc gagtccggcg
     6241 tcgagttctc gtcgacgttg gtcgagccgt agaagtcgtg gcggtgcgac acgcccacct
     6301 cgccggggta gacgatcggg tcgacctcgc cggagtgcga gtaggtgcac ttggcgacga
     6361 actggccggt tccgccctgc gggccggtgt ggcgggtcgg ggtggcgacg gtgccgtcgg
     6421 cccacactgg cgggtcatcg agcggcatgg accggtgacc ggccatgtcg tgggcgggcg
     6481 ccccgtcggc gagcgcgtcg gtgctcccga acgagccgtg gccgacggcg accgcggcga
     6541 tcagggcgag gacggcgagg agggcggccg caacggccgg tcccccacgt ccgaggtcgt
     6601 cgccgtcacc caccggtggg ggcggccggg agtttgatcg aggccaggtc gaccgtcatc
     6661 ggcttggtga acttgaccgg cttggccgtg gtggccttcg gcatgttctc ctgctccggg
     6721 ggctccggcg tcgccttgta cgagtcggcg ggcggcttgg gtgccggcga gcccatcagc
     6781 ccgatcaggt cgagcgccga ggcgttccag tccgtggcgg tcggctcgcc gttgcacggc
     6841 gcgagctgtt cgatctgggc cggttgcgac gtggtgaaca cgttgtccga gaagcagttg
     6901 cccaacttgt cggtggtctg acccgactcg gtcaggtcga ccgtgccgac ggccagatcc
     6961 gcgaccttgg agcccgatac gtcgttgccc tcgaccttga tctccatcgg gttccacatg
     7021 acgatgcagc cgtcgccgat gagctccacc tcgacgcact tgtcgggggc gatggtggtc
     7081 ttggccccgg actcagacgc cttcttggcc ggcgactccg aacacggctg gtcccactcg
     7141 gtctgcggcg gggccaggtc gttggcgtcg gactcgggga acggcaccag gccgatgccg
     7201 gtccggtcgt ggttccacac ccggttcctg gtgatctcga cccgcaccgc tcccgcgggc
     7261 aggatcccgt tgccctgggc gagcagcgac acgtcgatgc cggggccgtc ggtcatgttg
     7321 ttgtcgtgca ccaggttgcc ggcgatggtc gtgccgcggc tcgggtagca cagctcgtag
     7381 gaccccgagt tgggcacgat cccggcccgg ttgttgcgga actccgagtt gacgatgtag
     7441 aggtcaccgc cggagttggt gcccgagtag cccagcccgt tgtactccga gaccacctcg
     7501 ctcaacacgg cgtcgcactt gtagcactcg ccgatgtaga agccggcgtc ggggctgccc
     7561 gagccgtagc tgtggtcgaa ctgcccgtgg taggagtcga acgcgtagat gccgtagtcg
     7621 ccgttgcggt acgcggtgag gtacgagccg cggtagcggt cagagcccgt ccagtagaac
     7681 ccgttggaga tgtagttgcg ggcggtcatg ttctcgacca ccacgccgtc ggtgtcgagg
     7741 acgcggatgc cgttgtcggc tgtgaactcg ccgtcgagga tcaccttgtt gcgatccacg
     7801 ccgcggatgg tgatgtcggc cgtggtgacg tcgaccgcct ccttgtagac gccttcggag
     7861 accaggatca ggtcaccggc ctcggcggag tcgaccgcgg cctggatcgt cttctggtcg
     7921 cccggcaccc ggatcgcctc gaggccgtcc tcgaccgagg tcacccgggt cttggcgtcg
     7981 tcatcgccgc cgtcgccgct gtcgtcggag gagcacgccc cggcgaacaa cgccaccgcc
     8041 gcgatcgccg ccacaagcac cagccgtcgc cgtcccacca tggtccccct tggactcgat
     8101 ctcggatcga tctcggatcg atctgtcgcg acccgagcac tcttggatcg ggtcggtccg
     8161 taccctacag tccgtcgggt gaccagccga ggtatccgat ccatccgtct cgccctgatc
     8221 gccggcgtcg gcctgctcgc cgtcgcctgc ggaggcggga gctcgtcgtc gttctcgctg
     8281 cccgacggcg cgacgtggac caaggagtcc ggcaacgccg tcaccgtcga cacccgcgac
     8341 aacgtgttcg tcccgcagtt catcgaggtg aagaagggcg ccgaggtcac ctgggacaac
     8401 accgggcgca accgccacaa cgtgctgccc gacgaggaag gcgacttcaa ggaggtgcag
     8461 gtcgacgagc tggacccggg catgaaggcg tcgcgcacct tcgacgaggt cggcgagttc
     8521 ggctactact gctcgctgca cggcaccgcc accaagggga tgttcggcgc gatcaaggtg
     8581 gtcgagtagg tccggcgtcg agtagtcccg actcgagcgg ggcagcccga cgggtcgcgc
     8641 cgccgggtcg caccgtcggt ccgcggcgac ctgcaagtct gtacgagcca tgtcgttccg
     8701 tgaccgcttc ttcacgcccc gggtggcgcg ggcgaccacg tcaccgtcgg cgattctcgc
     8761 caccggcggc ggtgccgcgg ccgggatgct cgtcggcgga ccgatcgggg cgctgatcgg
     8821 cgggctcggc gcctacgccg tcagggttct cgccgcggtg ccccgggccc cgaagcaccc
     8881 cgggaccgac atccggggcc tggtcgatcc gtggcgcggc ctcatggtcg gggtgatcga
     8941 cgcccgtcgc cggttcgacc gggccgtctc gtcggtgcga cccggaccgc tgcgcgaccg
     9001 cctcggcgag gtcggcaccc gtctggacac cgcggtcgac gaggccggcc gcatcgcccg
     9061 ggccggcaac ctgttgacca gcggccgcaa gcagatcgac gtccccgcca tccgccagga
     9121 cctggatctg gcccgatccg cgcccccgac cgaccgcact gcggcgacga tccgggccgt
     9181 cgaggcacag ctcgagtcgg ccgggcggct cgaccgcacc atcgccgaca ccgtcgacca
     9241 gctccgcctg ctcgacgccc gcctggacga gacggtgacc cgcaccgtcg agctggccgc
     9301 cacccaggcc gactcgtcgg acctgggcgg catcggaccc gaggtcgagt cgatcgtgtc
     9361 cgagctggag tcgctgcggc aggccgtcga ggagaccggc ggccccactc cggctcccgg
     9421 gaccacgccg tgacccacga cgacgatctg gccggacacg acgatctggc cggacacgac
     9481 gacctggccc tcgccggact cgaggacgac gtcgcgctgg ccgaggccga ggcgggcgac
     9541 gacgtcatcg tcgaccggcg tcgcggccgg agcctgctcg cggccagcgc catgccggcc
     9601 gtgggcacca cgctcagccg ggcgacagga ctgatccggg tcggcgccct gacctacgcg
     9661 ctcggtctca cgacgatcgc ggacgtctac aacctggcca acaccacgcc gaacatcctc
     9721 tacgagctgg tgctcggcgg cgtcctgtcg tccaccctgg tcccgctgtt cgtgcggacc
     9781 ctcgacgacg cggacgacga caccgcctcg gtggtgacga ccgtcgcgtt ctcggccatc
     9841 gtggcgatga cgctgctcgg cgtggtcctc gccccgtgga tcaaccggct gttcgccctg
     9901 ccgttggaag gtgccgaccg cgcccagcag ctgcgcctgg gcgacgactt cctggagctc
     9961 ctgatcccgc agatcctgtt ctacggcgtc accacgctca tcacggcgct gctgcacgcc
    10021 cgccgccgct tcgctccccc tgcgttcaca ccgatcctca ccaacctggt caccgcggcg
    10081 gccgcggtcg ccgcagccaa gtggttcgcc cgcagcggcg ccgagatggg ccaggtctac
    10141 ctgctcgggc tgggcaccac cgcgggggtg gccgccatgg cgatcgccct ggtgccgtac
    10201 atccgcggtt cggggatcca gctgcggtgg cggttccagc cccgccaccc ggcggtgcgc
    10261 tcggtgatgc ggctctcggg ctggacggtc ggattcgccg cggccaacca ggtggccgtg
    10321 ctgttgatcc tgaccatggc ccgggcccag gaggcgggca cggtgtcggc ctaccagtac
    10381 gccttcatct tcttccagct cccccacggc ctgatcgcgg tgtcgctgat gaccgctgtg
    10441 ctgcccgagt tggccgaggc cgccgtcgac cgcgacgacc gggcctacat cgcccggttc
    10501 cgagagggca ccagcctgct gctcaccttc ctgctgccgg ccgcggtcgg cctgctgttc
    10561 gtgggcgagc ccctgatccg gctgttcctg cagcgcggca gcttcaccgc cgaggacacc
    10621 gagcggacca cgcggatgct gctcggcttc gcgatcggcc tcccggcgtt ctcggtgtac
    10681 ctgtactgcg tacgggcgtt cttcgcccgc cgcgacaccc ggaccccgtt cttcctcaac
    10741 gtgggccaga acgccctcaa cgtcgccctg gtcatcccgc tgacccggct gtggggggcc
    10801 gaggggttgt cggtcgccta ctcggcgtcg tactgggtca tcgcggtggt gacgctgtac
    10861 gtgttgaacc gtcgggtccc gcacctgctg accgcccggg ccatgtggcc gttcctgcgt
    10921 tcgatcggcg tgggcgtggc cgtgttcgtg gcgttggggg cgaccagcct ggtgttgggc
    10981 gaccaggtcg gcccggtgct cgaactgctg gtcgaggtcg gcgtggcggt cgcggtgttc
    11041 gtcccggtga cgttcatgct gaagcccaag ggcttcgagc cggccatcga ccggctccgt
    11101 cagggcatgc gccgccgggg tcgctcgatg gctggttagg ctcggcgcca tgtggaaagc
    11161 gatcaagcgg tggtgggcct acctgggcgc caaggtcaac agcgtcttca acgagaaggc
    11221 cgaccccaag gtgcagctgg agcaggcgat caccgaggct caggagcagc accgccggct
    11281 caaggagcag gcggccaacg tgatcgccaa ccagaagcag aaccagatgc gcctcgacgc
    11341 cgcgctcgag gaactggagc gcttgaaccg caacgcccag caggcggtgc tgatggccga
    11401 cgaagccacc cgcaagggcg acgccgccaa gaccgccgag tacacctccg ccgccgaggc
    11461 gttcgccaac cagctgatcg ccaaggagaa ggaggtcgag aacctcaagc agatgtcgct
    11521 gcaggccacc caggccgccg agcaggccaa ggcggcggtg cagcagaact cccgtgtgct
    11581 ccagcagaag ctggccgaga agcagaagct cctgggtcag ctcgaccagg ccaagatgca
    11641 ggagcagatg aacaaggcga tggcctcgct gtccgagcag gtcggtgagg acgtcccgac
    11701 gctcgacgag gtccgcgaga agatcgagac ccgctacgcc cgggccaagg ggatggccga
    11761 gctgcaggac atgtcggtcg agtcgaagat gctcgaggtc gagcaggc
//
LOCUS       DAOQOD010000099        11521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_1994_length_11521_cov_10.670330, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000099 DAOQOD010000000
VERSION     DAOQOD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_11521_cov_10.670330"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..2078)
                     /gene="recD"
                     /locus_tag="PLZ72_10385"
     CDS             complement(75..2078)
                     /gene="recD"
                     /locus_tag="PLZ72_10385"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNA36746.1"
                     /translation="MTDRFVPPSVAVLEPFVRAGVLGMVEVHVTATMVESVRSDPSRS
                     GGGSDGAGLPDEVVIAAALAVRAPLHGHVCVDLRHVAGSLVPAEGDAPTRRAVADVDG
                     RDDEGPDPDLDLPALAGDAAEMSPGAVELPWPDPDGWAGRVLASPLARADGAPAGDLV
                     EPLVVDGDALYLDRHWNDEVAVAADLRARAGVELDADAAAWAALDRYFPDPRSGGPDL
                     QRAAAVAALEHQLAVIAGGPGTGKTRTVARLLAVLASSDGSADGAGAGRPLEIALAAP
                     TGKAAARLQEAIAAAVREADPPAEVARRLASLDARTLHRLLGARRGGSTFARTASDPL
                     PADVVVVDEVSMVSLPLMARLLDAVRPDARVVLVGDPHQLASVEAGAVLGDVVGVQSG
                     RPPAALAANVVTLETVHRFAADSPVGRLASAIREGHVDGVIALLSGRGADGAVDDPDG
                     SVERVSWLDPGDDAGVDGVRRVVLAHAADLVDLGRAGRVDDALASLGSVKVLCATRRG
                     PAGVDTWNTWVEQQLRLGGRMRRRYSSGRPLMVTANDYLNEVFNGDVGVVVRVGGAGG
                     RPARPQVAFDGVDGARLIDPTRLDRLETQWAMTIHKSQGSEFDHVVVALPEPPSPILT
                     RELLYTAVTRAKRAVTVIASEAAVRAAVGRPVARASGLTARLS"
     gene            complement(2075..5494)
                     /locus_tag="PLZ72_10390"
     CDS             complement(2075..5494)
                     /locus_tag="PLZ72_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA36747.1"
                     /translation="MTVPEVIPFEVTEDLPSRGRLAVGASAGTGKTWTLAALATRYVA
                     ERGVPVGELLVVTFTRAAAAELRERIRTRFASTARDLEALAPGSEHPDPLVAHLLGGT
                     ADECAERRRRLERAVVDFDTATITTIHGFCAQVLAAQGNTGPFDPAAVLVQDPSEIVD
                     SVVADVLVEAALDPTPDPDSDPPGASIPLPSSSRLGALARIVLSNPGIRIVASNAKPT
                     DELVAELTRRVVERVDERLLLTGGVTFDDQLSRVAELLEQDADLRRTLSTQFTVVMID
                     ESQDTDPVQWAALSRAFEGSPSVTIVVGDPKQAIYSFRGGDVHNYLDAVGRAEQRRTL
                     TTNFRSDVAVVSALNRLAADAELGHPEIVYEKVEPAPALSGRSLHVRGAPAPGLWVRS
                     VTDPHQRETTKKLVDVSRARWTVAEDLAGRVVELVDHGEVVDADGTRKPVRPEDIAVL
                     IRARSDAAPVQRALLERGVASVVIGAGSVTSSLAASHWRWLIEGLKRPSDARLARAVG
                     TSWFFDVAVDDLAAGLGADPDGRGADTLTHIQESLLAWGDVLRRQGVAALVERVWRDG
                     DIRARVLSRPTGERDLTDLDHIAELLHLATGGRPVGAEVLGERFDALDDPDDEAVEDP
                     DAVRRRVDSDTSAVRIMTIHVAKGLEFPVVCCPTLWSMSYLDASSRTYHDPDSGDRVL
                     DVSDPPDAALKTHAQSEADGEHRRLTYVALTRALHTTLVWWVPAGPSDRSGLARVLFG
                     EGDAPGTAAVPDDWHAAVDVITDRVHELGADDVVRVELVPPDPPSVPMRRDLRSTVDA
                     DDRLELATLGRTLDRSAGRWSFTAMTARARHDAPTEHRPAGAGTVHDPDDPTLGDASD
                     GDEQHPAPPVLFDGLGAGAAFGTMVHSALEAVDFTGDLEGQLAEVLDVPWSNVEHDQV
                     PRLVRAIAATIHTPLGDAFGGLSLRSLARADRLDELDFELPLGRPGHPTVAASTIGEV
                     LADHLPGGDPLGDWSRRLAAGHIPVDVGGHLTGSIDLVLRAPADAPGGFRYGVVDYKT
                     NRLGSWGQPDTVANYHPDRLPAAMEEHDYPLQAVLYSVALHRYLRWRLPGYEPDVHLG
                     PVGYLFVRGMVGPDTPAPDGRTHGVFSWRVPAAAVVELSDGLDGHLPEVVS"
     gene            complement(5491..8970)
                     /locus_tag="PLZ72_10395"
     CDS             complement(5491..8970)
                     /locus_tag="PLZ72_10395"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNA36748.1"
                     /translation="MLQVHISDHVAPLVEVLAEQLAAPLEDPFASEWVAVPSIGMRRW
                     LTQQLSRRLGTQVGADGIAANVSMPFPDELRRAVLAADRVPAPSGSGMSAGEPLAVGM
                     VPEPDPWEPERLVWTVLEALSDPSSGGNPLLAPLADPPLGSSIVGRAQAIADLFDRYC
                     RHRPDMVRAWVDGRPVDGLGHPVPAGLRWQPELFAVVRDRLGLPSPPERLEQALRRLA
                     AGELDPALPPRLTLFGLSTLSSDLAQLVAAASAHFEVTLLFLAASPVAAAAVSAAVSA
                     DVTRSAPTAPIARRDDTSAELVHHPLLRSWGAAGRDAVITLASAGIDLAGAPVVGAVD
                     PAAADHAPHLLGRLRADLAADRAPEPTFRREMGDRSVQVHACSGPTRQVEVLRDTILH
                     LLADDPSLTESDIVVLCPRIEQFAPVIEAVFGPSADTVDTAAGAGRPPLLRYRITDRR
                     VRADVPLLGALAALLDLLPGRFSASAVADVLALAPISSRFGLGADDLDRLGDWVAQTN
                     IRWGIDGPHRLRWHLPADHRANTWAAGLDQLLMGTAVHGDDLTLTVGGVAPLAVGDSS
                     TRAAARVAEAVRTLADRRGHLVGLTAPVAQWCEVLADLVDRTCSLPWSESWQRRRLDR
                     VLRDLRDRSVGADGLPSELLLSLADVRRLLGGLLEGEPARAAFGTGAITCCSLSPLRA
                     VPARVVCLLGLDQDALPRGLAAGDDLLALAPRVGDRDPRSEIRQLLLDAVMSAQDALV
                     ITYDAVDVRTNLPVPAAVVLDELYDVLADTCDSGSDHLRSVLRVDHPRHGFDARNFRA
                     VGLGEPAGPAAGRPWGFDPHGLAGARSHRDRPRSPTVELLVPEPLDDLPADDVLTLDR
                     LREAAVRPVREMLTQRLGLHFPRAADDVVDQLPTDLDALARHRLGTDLLAARAQGWDT
                     DRWLAAMAARGALPPELLVADRLAEVTGVVDRIAGSAVELGVGLDPSERHPIDLDVAG
                     RRLVGVVERCASGDRPGPVTLTYSKAKAAQRIRAAVDLLVLVASDPEPDWRAVVVRRP
                     SGRTGLGDELLLVPRGETPDVRRRLAIDALSGLSAWRDAALRQPLPVAAETSRWLALG
                     KPSKAAATWNDTPAATGVMPGESSDAHIRAAFGELPFDALIDLEIDGRTAIGHAEALW
                     ELLDAAVDEEDRS"
     gene            9071..10303
                     /locus_tag="PLZ72_10400"
     CDS             9071..10303
                     /locus_tag="PLZ72_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNA36749.1"
                     /translation="MRLLHTSDWHLGRQFHQSSLLEEQAAAVDAMVELAASERVDAVL
                     IAGDLFDRAIPSTGAVDLFDDALVRLRDTGAVVVAISGNHDSASRAGFGDRLLTRAGV
                     TVRSDPARLADPVVVTDARSGDDSGAVAVYPVPFLDPLSVPRPLHDGPADDPDDADDL
                     PSPRRRPTHHSVMADAMDRIARDRRRRGLRSVVVAHAFVANFTPRLDEPPVEESDSER
                     ALAVGGSDRVDQSVFDHVDYVALGHLHGPQSWDDGRITYSGSPLAYSFSEQHHRKGVR
                     IVDLAADGQLDVAHVELGVGRGLTTLTGNLEDLLGRPEHADAEALRVRAVLTDAHLPG
                     GAMARLRQRFPYVAELLHQPPERTTSHDSPSSVQIRRRRPLDLAVEFVTEQWGDAPDA
                     AASGVLRRAVTAVAGADR"
     gene            10300..>11521
                     /locus_tag="PLZ72_10405"
     CDS             10300..>11521
                     /locus_tag="PLZ72_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA36750.1"
                     /translation="MRPLRLEIQAFGPYAGTQTIDFDDLAADGLFLIHGPTGAGKTAL
                     LDAIGFALYGQVPGSRQTRDLRSDHAPVDRPTEVRFEFLADGDHWLVRRRPGYVRPKR
                     RGTGTTTENPQSQLYKREGSDWKSVSRATLEVNRELRELIGLDHDQFSRVIVLPQGQF
                     QRVLRPSTAQEREELLTSLFDTELFGGIERWVHERWKESAASAAADRDRLVQLRHRAA
                     ERWTELVDAGTQSPLDSHGPLDLAGQDELDRLAENARRRAVDAADRAELHRGRLRAAA
                     DAHTATASAAERWDRRAALRDQAEQLEGDRARINELVERSAAGHAAVPLRDPLDAAEA
                     ATAAQAEAISEKVAAEAALRRAVRECPVPIDLLVTPSTAGVTVGGSGGEPADQLVAAV
                     ALEPDRALDRLRSRQ"
BASE COUNT         1642 a         4157 c         4321 g         1401 t
ORIGIN      
        1 acagttgagt accgccagtg tcgtgacttg tcgtcagaaa ctcggggttg gggggtgtgt
       61 ggaggggtgg ggcgtcagga gaggcgggcg gtgagaccgg aggcacgggc gaccggacgc
      121 ccgaccgcgg cgcgcaccgc ggcctctgac gcgatgacgg tgaccgcccg cttggcccgg
      181 gtcaccgcgg tgtagagcag ctcacgggtc aggatcggcg acggcggctc ggggagggcc
      241 accaccacgt ggtcgaactc ggagccctgg ctcttgtgga tcgtcatggc ccactgcgtc
      301 tccagccggt ccaggcgggt cgggtcgatc agacgggccc cgtcgacgcc gtcgaaggcc
      361 acctggggac gggcgggccg acccccggcg ccgcccacgc gcacgacgac gccgacgtcg
      421 ccgttgaaca cctcgttgag gtagtcgttg gcggtcacca tcaacggtcg gccagacgag
      481 taccggcgac gcatgcggcc gccgagacgc agctgctgtt cgacccaggt gttccacgtg
      541 tccacacccg ccgggccgcg cctcgtggcg cacagcacct tgaccgaccc cagcgaggcc
      601 agcgcgtcat ccacccggcc cgctcgtccg aggtcgacca gatccgccgc gtgggcgagc
      661 accacccgac ggaccccatc cacaccggcg tcgtcgccgg ggtccagcca gctcacccgc
      721 tcgaccgacc cgtcggggtc gtccactgcg ccgtccgcac ccctcccgga caggagggcg
      781 atgacgccgt cgacgtgacc ctcacggatc gcggacgcga gccggccgac cggggaatcg
      841 gccgcgaagc gatggaccgt ctccaacgtg accacgttcg cggccagcgc cgccggcggt
      901 cgaccgctct ggaccccgac cacgtcgccg aggaccgcgc cggcctcgac gctggccagc
      961 tgatggggat cgccgacgag caccacccgg gcgtcggggc gcaccgcgtc cagcaggcgc
     1021 gccatcaggg gaagcgacac catcgacacc tcgtcgacca cgacgacgtc ggcgggcaac
     1081 ggatccgatg cggtgcgggc gaaggtcgat ccgccccggc gcgccccgag cagccggtgc
     1141 agcgtccggg catcgagcga cgcaagacgt cgggccacct cggcgggagg gtccgcctcg
     1201 cggaccgcgg ctgcgattgc ctcctggagg cgcgccgcgg ccttgccggt cggcgcggcg
     1261 agcgcgatct cgagcgggcg accggcaccg gctccgtccg ccgacccgtc gctcgacgcc
     1321 agcacggcga gcagccgggc gaccgtgcgg gtcttgccgg ttccggggcc tccggcgatc
     1381 accgccagct ggtgctccaa cgccgcgacc gccgcggccc gctgcaggtc gggtccgccg
     1441 gagcgggggt ccgggaagta ccggtccagc gccgcccacg ccgcggcatc ggcatcgagc
     1501 tcgacgcctg cccgggcccg gagatcggcg gcgacggcca cctcgtcgtt ccagtgccga
     1561 tccaggtaca gggcatcgcc atcgaccacc agcggttcga caagatcccc cgcgggcgcg
     1621 ccgtcggcgc gggcgagcgg gctggccagg acccggccgg cccagccgtc cggatcgggc
     1681 cacggcaact ccaccgcgcc gggcgacatt tcggcggcgt cgcccgcgag cgcggggaga
     1741 tcgaggtcgg ggtccgggcc ttcgtcgtca cgaccgtcga cgtcggcgac cgcccgacgg
     1801 gtgggagcgt cgccctccgc ggggaccagg gagccggcga cgtggcggag atcgacgcag
     1861 acgtggccgt gcagcggggc ccgcaccgcc agggcggcgg cgatcaccac ctcgtcgggc
     1921 agccccgccc cgtcggaccc gccgcccgac cgcgacggat ccgaccggac cgactcgacc
     1981 atggtggcgg tgacgtggac ctcgaccatg ccgaggaccc cggcccgcac gaacggctcg
     2041 aggacggcca ccgacggcgg gacgaagcga tcggtcacga cacgacctcc gggaggtgcc
     2101 cgtccaaccc gtcgctcagc tcgaccacgg ccgccgcagg gacccgccag gagaacacgc
     2161 cgtgggtccg gccgtcggga gccggggtgt cgggcccgac catgccccgg acgaacaaat
     2221 agccgaccgg ccccaggtgg acgtcgggct cgtagccggg cagacgccac cggaggtagc
     2281 ggtgcagcgc gaccgagtac aggacggcct gcaacgggta gtcgtgctcc tccatggccg
     2341 cgggcaggcg gtccgggtgg tagttggcca ccgtgtcggg ctgcccccag gaccccagcc
     2401 ggttggtctt gtagtcgacg actccgtagc ggaacccgcc gggcgcgtcc gcgggagcac
     2461 ggaggacgag gtcgatcgag ccggtcaggt ggccgccgac gtccaccggg atgtggccgg
     2521 ccgccagccg ccgggaccag tcccccaacg ggtcgccgcc gggcaggtga tcggcgagga
     2581 cctcgccgat cgtggacgcc gcgacggtcg ggtgcccggg gcgaccgagc ggcagctcga
     2641 agtcgagctc gtcgagacgg tcggcgcggg ccagcgagcg cagcgacagc ccgccgaacg
     2701 cgtcgccgag cggcgtgtgg atcgtcgccg cgatcgcccg caccaaccgc ggcacctggt
     2761 cgtgctcgac gttcgaccac ggcacgtcga gcacctcggc gagctggccc tccaggtcgc
     2821 cggtgaagtc gacggcctcg agcgcggagt ggaccatggt gccgaacgcc gcgccggcgc
     2881 cgaggccgtc gaacaggacc ggcggcgcgg ggtgctgctc gtcgccgtcg gaggcgtcac
     2941 ccagggtcgg gtcgtcgggg tcgtgcacgg tccccgcccc ggccggtcgg tgctcggtcg
     3001 gtgcgtcatg acgggcgcgg gcggtcatgg ccgtgaacga ccaccggccg gcagaccggt
     3061 cgagggtccg gccgagcgtc gccagctcca ggcggtcgtc ggcgtcgacc gtggagcgga
     3121 ggtcccggcg catcgggacc gatggcggat ccggaggcac cagttcgacc cggacgacgt
     3181 cgtcggcgcc gagctcgtgg acgcggtcgg tgatgacgtc caccgccgcg tgccagtcgt
     3241 cggggaccgc tgcggtcccc ggcgcgtccc cctcgccgaa cagcacccgg gccaatccgg
     3301 agcggtcgct gggaccggcg ggcacccacc agaccagcgt ggtgtgcaac gcgcgggtga
     3361 gcgcgacgta ggtgagccgt cggtgctcgc cgtcagcctc gctctgtgcg tgcgtcttga
     3421 gggcggcgtc gggtgggtcg ctgacgtcga ggacccggtc accgctgtcc gggtcgtggt
     3481 aggtgcggga gctggcgtcc aggtaggaca tgctccacag cgtggggcag cacacgaccg
     3541 ggaactcgag gcccttggcg acgtggatgg tcatgatccg caccgcggag gtgtcggagt
     3601 cgacccgtcg acggacggcg tcggggtcct cgaccgcttc gtcgtcgggg tcgtcgagcg
     3661 cgtcgaaccg ctcgccgagg acctccgctc cgaccgggcg accgcccgtc gccaggtgca
     3721 gcagctcggc gatgtgatcc aggtcggtca ggtcccgctc gccggtgggt cgcgacagga
     3781 cccgggcccg gatgtcgccg tcgcgccaga cccgttcgac cagcgccgcg accccctgac
     3841 ggcgcaggac gtcgccccaa gccagcagcg actcctggat gtgggtgagg gtgtcggcgc
     3901 cgcgcccgtc ggggtccgcg cccaggccgg cggccaggtc gtcgacggcg acgtcgaaga
     3961 accacgacgt gccgaccgcc cgggccagtc gggcgtcgct cggacgcttc aacccctcga
     4021 tcaaccaccg ccagtgcgac gccgccagcg agctggtcac gctcccggcg ccgatgacga
     4081 ccgacgccac cccccgctcg agaagcgctc gctggacggg agcggcgtcg ctgcgagccc
     4141 tgatgagaac ggcgatgtcc tcgggccgca ccggcttgcg ggtaccgtcg gcgtcgacga
     4201 cctcgccgtg gtcgacgagc tcgacgaccc gaccggcgag gtcctcggcg acggtccagc
     4261 gggcacggga gacgtcgacc agcttcttgg tggtctcccg ctggtgcggg tcggtcaccg
     4321 aacgcaccca cagccccggg gcaggtgcac cccggacatg gagtgagcgc ccggacagcg
     4381 cgggagcggg ctcgaccttc tcgtagacga tctccgggtg tccgagctcg gcgtccgcgg
     4441 cgaggcggtt gagggcggac accaccgcga cgtcggagcg gaagttggtg gtgagggtgc
     4501 gacgctgctc ggcccgaccg accgcgtcca ggtagttgtg gacgtctccg ccccggaagc
     4561 tgtagatggc ctgcttgggg tcgccgacga cgatcgtcac cgacggggag ccctcgaagg
     4621 ccctcgacag agcggcccac tggacgggat cggtgtcctg gctctcgtcg atcatcacca
     4681 ccgtgaactg ggtcgacaga gtgcggcgga ggtccgcgtc ctgctccagc agctcggcga
     4741 cccggctgag ctggtcgtcg aaggtgacgc cgccggtcag cagcagccgc tcgtcgacgc
     4801 gctccaccac ccgtcgggtg agctcggcga ccagctcgtc ggtgggcttg gcgttgctgg
     4861 cgacgatcct gatgcccggg ttggacaaca cgatgcgagc cagggccccg agccgcgacg
     4921 atgacggcaa cggtatcgat gcacccgggg ggtcgctgtc cggatcgggt gtgggatcca
     4981 acgcggcttc gaccagcacg tcggcgacga ccgagtcgac gatctcggag gggtcctgga
     5041 cgaggacggc cgcgggatcg aacgggcccg tgttgccctg ggcggcgagg acctgggcgc
     5101 agaagccgtg gatggtggtg atggtcgcgg tgtcgaagtc gaccacggcg cgctcaagcc
     5161 ggcgccgtct ctcggcgcac tcatcggcgg ttccaccgag gaggtgggcg accaggggat
     5221 cgggatgctc cgatcccggc gcgagagcct ccaggtcgcg ggccgtcgac gcgaagcggg
     5281 tccggatgcg ctcgcgcagc tcggccgcgg ccgcccgggt gaaggtgacc acgagcagct
     5341 cccccaccgg gacgccccgc tcggccacgt aacgggtggc gagcgcggcg agggtccagg
     5401 tcttcccggt tccggcgctg gcaccgaccg ccagacgccc ccgggagggg aggtcctcgg
     5461 tcacctcgaa cgggatcacc tccgggacgg tcacgaccga tcctcctcgt cgacggcggc
     5521 gtcgaggagc tcccagaggg cctcggcgtg gccgatcgcg gtccgaccgt cgatctccag
     5581 gtcgatcagc gcgtcgaagg ggagctcgcc gaacgcggcg cggatgtggg cgtcggagct
     5641 ctcaccaggc atgacaccgg ttgccgcagg ggtgtcgttc catgtcgccg ctgccttgct
     5701 cggcttcccc agggccaacc aacgactggt ctcggccgcg acgggcagcg gttggcggag
     5761 agccgcgtcg cgccaggcgg agagtccgct cagggcgtcg atcgcgaggc ggcggcgcac
     5821 gtcgggtgtc tcaccgcgcg ggaccaggag caactcgtcg cccaggcccg tcctgcccga
     5881 cggacgccgg accacgacag cccgccagtc cggctcggga tcggacgcga ccagcaccag
     5941 caggtcgacc gcggcacgga tccgctgcgc ggccttggcc ttggagtacg tcagcgtgac
     6001 cggacccggc cggtccccgg atgcgcagcg ctcgacgacg ccgaccaggc gccggccggc
     6061 cacgtcgagg tcgatcgggt gccgttcgga cgggtcgagg ccgacgccga gctcgaccgc
     6121 cgaccccgcg atccgatcga cgaccccggt cacctccgcg agccgatccg cgaccagcag
     6181 ttcggggggc agagcacccc tggccgccat ggccgccaac caacgatcgg tgtcccatcc
     6241 ctgcgcccgc gccgcgagca ggtcggtgcc gagccggtgc cgggccaggg catcgaggtc
     6301 ggtggggagc tggtcgacca cgtcgtcggc ggcgcgcggg aagtgcagcc cgagccgttg
     6361 ggtcagcatc tcccggaccg ggcgcacagc ggcttcgcgc aaccggtcga gcgtcagcac
     6421 gtcgtcggcc ggcaggtcgt cgagcggttc ggggacgagc agctcgacgg tgggcgatcg
     6481 cgggcggtcc cggtgagagc gtgccccggc caggccgtgg gggtcgaatc cccacggtcg
     6541 acccgcggcc ggacccgccg gctcgccgag gccgacggcc cggaagttgc gggcgtcgaa
     6601 gccgtggcgg gggtgatcga cccggaggac cgaacggaga tgatcactcc cgctgtcgca
     6661 cgtgtcggcg agcacgtcgt acagctcgtc gaggacgacc gcggccggga ccgggaggtt
     6721 tgtccggacg tcgaccgcgt cgtaggtgat caccagcgcg tcctgcgcgg acatgaccgc
     6781 gtcgagcagg agctgacgga tctccgagcg tgggtcccgg tcaccgaccc gcggcgccag
     6841 ggcgaggagg tcgtcgccag cggcgaggcc gcggggtagc gcgtcctggt cgaggccgag
     6901 caggcacacg acgcgggccg gcaccgcgcg caacggcgac aacgagcaac aggtgatcgc
     6961 tccggtgccg aacgccgccc gggccggctc accctcgagc aggccaccga gcagccgcct
     7021 gacgtccgcg agcgacagca ggagctccga cggcagaccg tcggcgccga cggatcggtc
     7081 gcgcaggtcg cgcagcaccc ggtcgaggcg gcggcgttgc cacgactcgg accacggcaa
     7141 cgagcaggtg cggtcgacca gatcggcgag gacctcgcac cactgcgcca ccggagcggt
     7201 gagtccgacc aggtggccgc gccgatcggc cagggtgcgg acggcctcgg ccacacgggc
     7261 cgcggcacgg gtggagctgt cgccgacggc gaggggggcg acacctccga cggtgagggt
     7321 gaggtcgtcg ccgtggaccg ctgtgcccat gaggagctgg tccaggcccg ccgcccatgt
     7381 gttggcccgg tgatcggcgg gcaggtgcca gcggaggcgg tgcggcccgt cgatgcccca
     7441 acggatgttg gtctgggcca cccagtcccc gagccggtcg aggtcatcgg cgccgagacc
     7501 gaaccgtgag ctgatcgggg cgagcgccag gacgtcggcg accgccgacg ccgagaagcg
     7561 cccggggagc aggtccaaca acgcggcgag cgcaccgagg agggggacgt cggcgcggac
     7621 gcgtcggtcg gtgatgcggt agcgcagcag cggcggacgg cccgctccgg cagcggtgtc
     7681 gacggtgtcg gccgacgggc cgaacacggc ctcgatgacc ggggcgaact gctcgatgcg
     7741 ggggcacagg acgacgatgt cggactcggt cagcgacggg tcgtcggcca ggaggtgcag
     7801 gatggtgtcg cgcaggacct cgacctgacg ggtcggaccg gagcaggcgt ggacctgcac
     7861 cgagcggtcc cccatctccc gacggaacgt cggttccggg gcgcggtctg ccgccagatc
     7921 ggcacggagg cgaccgagca ggtgcggggc gtgatcggcg gcagcggggt cgacggcgcc
     7981 gacgacggga gctccggcca ggtcgatgcc agcggacgcg agggtgatca cggcgtcgcg
     8041 tccggccgct ccccacgagc gcagcagcgg gtggtgcacc agctcggcgg aggtgtcgtc
     8101 acgacgggcg atcggagcgg tcggcgccga ccgtgtgacg tcggccgaga ccgctgccga
     8161 gaccgcggcg gcggccaccg gcgacgcggc gaggaacagc agcgtcacct cgaagtgcgc
     8221 ggatgccgcc gcgaccagct gggccaggtc cgacgacagg gtgctcagcc cgaacagggt
     8281 gagtcggggc ggcagagcgg gatcgagctc tccggcggcc aggcggcgca gcgcctgctc
     8341 gagccgttct ggcgggctgg gcaggccgag tcggtcccgc acgacggcga agagctccgg
     8401 ctgccagcgc agaccggcgg gtaccgggtg gccgagcccg tcgaccgggc gaccgtcaac
     8461 ccacgcccgg accatgtcgg ggcggtgtcg gcagtaccgg tcgaacaggt ccgcgatcgc
     8521 ctgggcccga ccgacgatcg aggagccgag cggtggatcg gccagcggag cgagaagcgg
     8581 gttcccgccc gacgacgggt ccgagagcgc ttcgagcacg gtccacacca gacgttcggg
     8641 ctcccatgga tcgggctccg ggaccatccc cacggcgagc ggttcgccgg ctgacatccc
     8701 ggacccggat ggggccggga cccgatccgc agcgagcacg gcgcggcgca gctcgtcggg
     8761 gaacggcatg gacacgttgg cggcgatgcc gtcggcgccc acctgcgtgc cgagccggcg
     8821 ggacagctgc tgggtgagcc agcggcgcat gccgatggac ggtacggcca cccactcgga
     8881 ggcgaagggg tcctcgagcg gcgcggcgag ctgctcggcc agcacctcga cgaggggggc
     8941 gacgtgatcg gagatgtgca cctgcagcac ggacggcgac gctaccggtc ggggctgaca
     9001 gtcgagcccg tggcggtgtc ggccgggccc catccgcggc gttgtcacac cccctcggta
     9061 cgttcgcccc atgcggttgt tgcacacctc cgactggcac ctgggacgtc agttccacca
     9121 gtcgtcgctt ctcgaggagc aggccgccgc ggtcgacgcc atggtcgagc tcgcggcgtc
     9181 cgaacgggtc gacgccgtgc tcatcgcggg cgacctgttc gaccgggcca tcccctccac
     9241 cggggcggtg gacctcttcg acgacgccct cgtccgcctc cgcgacaccg gagcggtggt
     9301 cgtggccatc tccggcaacc acgactcggc gtcgcgggcc gggttcggcg accgcctgct
     9361 gacccgggcc ggtgtgaccg tgcgttcaga tccggcccgg ctggccgatc cggtcgtggt
     9421 caccgacgcc cgcagcggcg acgacagtgg ggccgtcgcg gtctaccccg tcccgttcct
     9481 cgacccgctg tcggttcccc gccctctcca cgacggcccc gccgacgatc ccgatgacgc
     9541 ggatgacctc ccctcgccgc ggagacggcc cacccaccac agcgtcatgg ccgacgcgat
     9601 ggaccggatc gcccgggacc gacgccgtcg cgggctgcgg tcggtggtgg tcgcccacgc
     9661 cttcgtcgcc aacttcactc cccgcctcga cgagccaccg gtcgaggaga gcgacagcga
     9721 acgggcgctc gccgtcgggg gatcggaccg ggtggaccag tcggtcttcg accacgtcga
     9781 ctacgtcgcg ctcggacatc tccacgggcc gcagtcctgg gacgacggcc gcatcaccta
     9841 ctcgggctcc cctctcgcgt actcgttctc cgaacagcac caccgcaagg gggtgaggat
     9901 cgtggacctg gccgcggatg gtcagctcga cgtcgctcac gtcgagctgg gggtcggtcg
     9961 gggtctcacc acgctcaccg ggaacctcga ggatctcctc ggtcgtcccg agcacgcgga
    10021 cgcggaggcg ttgcgggtcc gtgccgtgct caccgacgcg cacctgccgg gcggggccat
    10081 ggcccgcctg cgccagcgct tcccgtacgt cgccgaactg ctccaccagc ccccggagcg
    10141 caccacatcg cacgactccc cctcctcggt gcagatccgg cgccgacgtc cgctcgacct
    10201 ggccgtcgag ttcgtcaccg agcagtgggg cgacgcgccc gacgcggcgg cgtcgggcgt
    10261 gctgcgccga gcggtcaccg cggtcgcggg agcggaccga tgaggccgtt gcgactggag
    10321 atccaggcgt tcggccctta cgccgggacc cagacgatcg acttcgacga cctggcggcc
    10381 gacggcctgt tcctgatcca cgggcccacc ggcgccggca agaccgctct gctcgacgcg
    10441 atcggcttcg ccctctacgg ccaggtgccc ggcagccgcc agacccgcga cctccgctcc
    10501 gaccatgcac cggtcgaccg gcccaccgag gtgcgcttcg agttcctcgc cgacggcgac
    10561 cactggctcg tgcgccgccg gccggggtac gtgcgaccca agcgccgtgg caccggcacc
    10621 accaccgaga acccgcagtc ccagctctac aagcgggagg gctccgactg gaagtccgtc
    10681 tcacgcgcca ccctcgaggt caaccgtgag ctccgggagc tgatcggcct cgaccacgac
    10741 cagttcagcc gcgtcatcgt gctgccgcag ggtcagttcc agcgcgtgct gcggccctcg
    10801 accgcacagg agcgtgagga gctgctcacg tcgctgttcg acaccgagct gttcggtggc
    10861 atcgaacgct gggtgcatga acggtggaag gaatccgcgg cgtcggcggc cgccgatcgg
    10921 gaccgcctcg tgcagttgcg tcaccgcgcc gccgaacgct ggacggagct ggtcgatgcc
    10981 ggaacgcagt cgccgctgga ctcacacggc ccgctggacc tcgccggcca ggacgagctc
    11041 gaccgcctcg ccgagaacgc ccgacgccgt gcggtcgacg ccgcggaccg cgccgagctg
    11101 caccgcggtc gactccgggc agcggccgac gcgcacaccg cgaccgcgtc ggcggccgag
    11161 cgatgggacc ggcgtgccgc cctgcgggat caggccgaac agctcgaagg agaccgggcc
    11221 cggatcaacg agctggtcga acgctcggcc gcggggcacg ccgcggtccc gctccgggac
    11281 ccgttggacg cggccgaggc cgccaccgcc gcgcaggccg aagccatctc ggagaaggtc
    11341 gcagccgagg ccgccctccg gcgggcggta cgtgagtgcc cggtcccgat cgacctgctg
    11401 gtcaccccct ccaccgcagg agtcaccgtc ggcggctcgg gcggcgagcc ggccgatcag
    11461 ctggtggccg ccgtggcact ggagccggac cgggccctgg accgactccg ttcccgacag
    11521 g
//
LOCUS       DAOQOD010000100        11240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2056_length_11240_cov_10.857309, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000100 DAOQOD010000000
VERSION     DAOQOD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_11240_cov_10.857309"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLZ72_10410"
     CDS             <1..208
                     /locus_tag="PLZ72_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36751.1"
                     /translation="ASGAAAASHVLAAIGADAASDGRRRGALRLTLGHDATDGQVDRA
                     VEVIAATVERLRAPVRRLTPDREG"
     gene            210..1328
                     /gene="mnmA"
                     /locus_tag="PLZ72_10415"
     CDS             210..1328
                     /gene="mnmA"
                     /locus_tag="PLZ72_10415"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNA36752.1"
                     /translation="MRVLAAMSGGVDSSVAVALLQERGHDVVGVTMRLWGGPSDTGCC
                     SATEVADARRAAARLGVEHHVFDFSEEFDRHVVDPYVADHAAGRTPNPCVECNRHLKF
                     DALVQRADVLGFDAVATGHHARIVRDADGRHLVARGVDVAKDQSYVLHPIVDSDLGRV
                     LMPVGEMTKDRVRARAAALGLRTATKPDSQDVCFITRAEGRAAFLAERMALHPAEIRD
                     VDGRTVGRTDAVELVTIGQRRGLGLAGGADRRFVLDVDVAARTVTVGPPERLVGDGVA
                     LERVRWAGGRPLAPGTEVSVQCSAHGEVRPATVETAGGTSATIRWRSPQRRVAPGQSV
                     VLFGATANGVEVVLGGGTSASGDGDPSGLRESQQLPGS"
     gene            complement(1266..2156)
                     /locus_tag="PLZ72_10420"
     CDS             complement(1266..2156)
                     /locus_tag="PLZ72_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36753.1"
                     /translation="MHVTPLTWITRALWVLLPFTLGALLGDAAAEASTAGADTALGVA
                     VAAWAVWVLGLFALFVLLPDALVALRVVAPLPLLAGAVAAVAQTPGALGWIGLAVAAA
                     VAVLSMSADVGSDFVNGSSYGDEHRVALRPPTALLVGPIEAVWAGTAVPLPVAVVLGV
                     SRQWAGAAVLGVVGVGLALVGFRALSRLARRWLVLVPAGITVVDPMALAEPILLRRTS
                     IARLGPAPADTDALDLTVGAGGLILQVDLSTPVELVPAPSRRGAVAEPQEVAAVLVAP
                     SRPGELLRLAESRRIAVARR"
     gene            2207..3208
                     /locus_tag="PLZ72_10425"
     CDS             2207..3208
                     /locus_tag="PLZ72_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36754.1"
                     /translation="MAEGADLPFGLASAVGPVPFASADDAVAFALRRPRYPTVPVTSD
                     PSASLWAQAVSGLDDGVGADAAGADLSGPAFAAARAFVEALAHLDVASSPVAIRVPVL
                     GPVTVASELRRLGRPAEDADRIAVEVVSARAVALPGLIRAAGAVPTSAAAPVVSVVLE
                     EPALVGSMHPTFPRRPAEIRALLDPVIDALDRAAPPRSLLIGVHVAGPCDWPSVIGSG
                     ASLLCVPVDRTVSGWAPLFGDLLDRGGRICWGVVPVDRPLGRSTDRYWRRLVALWTAM
                     VAEGVDPMALRLRSSFSPADGLDAFGPSQAELVMELTAEVAERVSHQAVAARLSLGA"
     gene            3254..5377
                     /gene="ligA"
                     /locus_tag="PLZ72_10430"
     CDS             3254..5377
                     /gene="ligA"
                     /locus_tag="PLZ72_10430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNA36755.1"
                     /translation="MTAAGSTADPDRDRSADGEMDPVGRIDDLREQIRGHDRRYYQLD
                     APTIPDADYDALVRELRSLEVQHPDLITPDSPTQTVGGPVLTQFSPVTHAVRMMSLDN
                     AMDRDELRAWGERTDRRLASLGLDGTVRYVCELKIDGLAMSLRYEDGDLVQAATRGDG
                     RTGEDVTANVSTISDIPTRLGAHAPTVLEVRGEVYLPLEAFRRLQEQTVADNAAADAA
                     GRKGRPVPVNPRNAGAGSLRQKDPTVTASRGLSFWAYQLGQVVGADTPPTHSAALDWL
                     RTLGLPVNPETRILDSLDEVYDFCDHWVEHRHDLPYEIDGVVVKVDDLSVQSALGSTS
                     KAPRWAIAYKLPPEERTTTLLDIQVSIGRTGKATPFAVLEPVFVGGSTVGLATLHNQD
                     QVRLKDVRPGDTVIVRKAGDVIPEVVGPVLELRPDGLAEWAFPTECPSCGGPLVRSEG
                     EAQTRCVNPDCPQKVLARIAHFGSRGAMDIEGLGEQQVQLFLELGLLHDVADVYSLDY
                     ERIRELPRYGDTSVRNLRAAVEASKSRPLANLLFGLNITHLGQAGGEALAEGLGSLPA
                     IMDASVDDLAAVDGVGPVVAASIHDFFGDPANRRLVQRLIDAGVNIQGPERSVLPQTL
                     SGRSVVVTGSVPGHSRESAEAAVKARGGKSPGSVSKKTFAVVVGDDPGASKVTKAESL
                     GVPMVSAALFEELLETGEIPTGGGS"
     gene            5380..6015
                     /locus_tag="PLZ72_10435"
     CDS             5380..6015
                     /locus_tag="PLZ72_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNA36756.1"
                     /translation="MQAVVFDFGGVFIDSPFDGVAAAARRHGFDPQELSDIVFGPYDQ
                     DTDHPWHRLERGEISVSDARDAIVVASVADGGTEVDPFVVLAGIAGGEVRDEMVAFCR
                     EVRSAGLRTGLLTNNAKEFETYWRPLLPLDELFDDVVDSSAVGLRKPDPRIYELSLSR
                     LGVTAADAVFIDDAPGNVDGARAVGMSAVLIGQQRADVPAALAELRSLVAL"
     gene            complement(6033..6560)
                     /locus_tag="PLZ72_10440"
     CDS             complement(6033..6560)
                     /locus_tag="PLZ72_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36757.1"
                     /translation="MHDDPPSDGDHADDDPAELTAGPTAAASPARKERRELPDRWAYS
                     IMALLIVGSLVGIVVAVMLASTGGDRTSEVLPEYVDRLEPTSGSHILSQSTVGVDVAE
                     GYDAYLIIDGKEIKTQADGLIRQPNKGIVQYTPGEGRPVESLPSGRNCVTAMVWKITD
                     GPKTAKPTNWCFEVT"
     gene            6668..8794
                     /locus_tag="PLZ72_10445"
     CDS             6668..8794
                     /locus_tag="PLZ72_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNA36758.1"
                     /translation="MSSPPTSSHRAIAEIGRELGGRYRLVAPIGVGTSSRVFLAVDTQ
                     LRRRVAVKLLHAALAADDTFLRRFRAEARAAGALNHPNIVAVFDWGEDETDEGMQPYL
                     VTEYLGGGSLRRILDRGRPLSPSQALMVGLDTARALAHAHRRGLVHRDVKPGNLLFDT
                     EGRLRLADFGVARALAEAAWTEPSGTMLGTARYASPEQAMGRRLDGRSDVYSLALVLI
                     ECVTCQVPFAADTTTGTLALRTQGDLEVPDGLAGLRGTLERAGRLEPDQRPDAEEVEI
                     SLLAAAETMPRPEPLPLVATLDEAEITAELRLVEGIDGLGVVSALDADEPPVPDDAVT
                     GRAEDVDRTVDEPEVLERTPIVVAEEHHLLATSRPASFGRVEPFVADARVGGDPGAAD
                     PGVAPIGPMPSARRRRRAPRLLLGVVAALALLGAAAAWWFLIRVPVHDVPDLVGRPLA
                     EASALARQNGWKLDDDTVVRVDDTRPGQVVEQSPRSGSTLSEGDTLHVTVSLGPTLVA
                     VPDLKGVPEAEARAQIEAAGLTAGPSSTTTDEEVPVGAVVSAGPAPGQEAPASDGTVP
                     RGTELALVVSSGPAPRVVPDGLVGLSVADAKARLAAVQLGAAVTEVNDEVAPAGMVMA
                     VGTPPGTSVPRDTAVALTVSKGPAPIPVPDVVGLAGTAASAQIQAVGLGVSGIEGSPS
                     NAVLATDPPAGEAIPRGAPVRIFTRR"
     gene            8970..10502
                     /locus_tag="PLZ72_10450"
     CDS             8970..10502
                     /locus_tag="PLZ72_10450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA36759.1"
                     /translation="MVDPPQPQDPLHDPITDVAVEAPGVDEEAVVPWSMLLRRRPTGP
                     AESGSGSDRPWLVTWTVLFGAFWVSSTITILAVSRPEIAQDLGASVESLVWLISGPTI
                     AVALTGTTAGKLGDLHGHRRVYLIGMVGATVFVALTALAWSGPSLIAFRVLGATIGAA
                     TGPSSLALINRMFTPERRSRALGFWSLVVAGGPVVGLVAGGPLVEAFGWRVIFAVQAP
                     LLAVATVVAFVVLPETGRRRDVHFDVPGQLWLTVTLGGLLFGIDRAAAWGLTNPWTIG
                     FFLLVPVGFLSFLARERRAEHPLIPLDWFARRSFSVPVGVSFFMQFGYMGGFILTPLL
                     LEQVRDLPADVVALIMVPRPLTFAVAGPLAGFMVGRVSARSTIVAGSASLALSMVVFA
                     LVAEDPTTAVVVLALTLSGIGVGAAQPAIASSVANSVPNRDLGIAGATQQLVSQVGTT
                     LGMNVLEAVESVSRDGRGPGGSYSIAYAVGAAVAGAGVVLSLFMTRGPDADRHPTTTP
                     DG"
     gene            complement(10548..10961)
                     /locus_tag="PLZ72_10455"
     CDS             complement(10548..10961)
                     /locus_tag="PLZ72_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNA36760.1"
                     /translation="MPKYQFLTPEWIEEAKKIREQFDSDATATPHVVRMNQVITEVPF
                     GDGTVEAHMDTTSGEVKMDLGHLESPDLTVTIDYVTAKAIIVEGNPQAGMQAFMAGKV
                     KVQGDMTKLMAMQQPVGGQDETAAKVAAAIQEITE"
BASE COUNT         1399 a         4133 c         4088 g         1620 t
ORIGIN      
        1 cgcgtcggga gcggcggccg cgtcgcacgt gctcgccgcc atcggcgccg acgctgcctc
       61 agacggccgc cgccggggag cgctgcggtt gaccctcggc cacgacgcca ccgacggcca
      121 ggtcgaccgg gccgtcgagg tgatcgctgc caccgtcgaa cggctccgcg cccctgtacg
      181 ccgcctgacc cccgaccggg aggggtgacg tgcgcgtgct ggccgccatg tccggcggcg
      241 tcgactcgtc ggtggcggtc gcgctgcttc aggagcgggg ccacgacgtc gtcggggtca
      301 ccatgcgcct gtggggcgga ccgtccgaca ccgggtgctg ctcggcgacc gaggtggccg
      361 acgcccggcg ggccgcggcc cgactcggag tcgagcacca cgtcttcgac ttctccgagg
      421 agttcgaccg ccacgtcgtc gacccctacg tggcggacca cgccgccggc cgcaccccca
      481 acccgtgcgt cgagtgcaac cgccacctca agttcgacgc ccttgtgcag cgcgccgacg
      541 tgctgggctt cgacgcggtg gccaccggcc atcacgcccg catcgtgagg gacgcggacg
      601 ggaggcacct ggtggcacgg ggcgtcgacg tcgccaagga ccagagctac gtcctgcacc
      661 cgatcgtcga ctcggatctg gggcgggtgc tcatgccggt cggcgagatg accaaggacc
      721 gggtccgggc ccgggccgcg gcgctggggt tgcgcaccgc caccaagccc gacagccagg
      781 acgtctgctt catcacccgc gccgagggcc gggccgcgtt cctggccgag cggatggcgt
      841 tgcaccccgc cgagatccgc gacgtcgacg gccgcaccgt ggggcgaacc gacgccgtgg
      901 agctggtgac gatcggccag cggcggggcc tgggcctggc cgggggagcg gaccgccggt
      961 tcgtgctcga cgtcgacgtc gcggcccgca ccgtgacagt gggtccgccc gaacggctcg
     1021 tcggcgacgg cgtcgcgctc gaacgggtgc gctgggccgg aggccgaccg ctcgcccccg
     1081 ggaccgaggt gtccgtgcag tgctcggccc acggcgaggt gcgacccgcg acggtcgaga
     1141 ccgccggcgg caccagcgcc acgatccgat ggcggagccc ccagcggcgg gtggcgcccg
     1201 gtcagtcggt cgtgctgttc ggtgcgaccg cgaacggcgt cgaggtcgtc ctcggcggcg
     1261 ggacctcagc gtcgggcgac ggcgatccgt cgggactccg cgagtcgcag cagctccccg
     1321 ggtcgtgacg gggcgacgag caccgcagcg acctcctgcg gttcggccac cgctccccgg
     1381 cgggacgggg cggggaccaa ctcgaccggg gtcgacaggt cgacctgcag gatcagtccg
     1441 ccggctccga cggtgaggtc gagggcgtcg gtgtcggccg gtgccggccc gagccgtgcg
     1501 atcgaggtcc ggcgcaggag gatcggttcg gccagcgcca tcgggtcgac caccgtgatc
     1561 cccgccggca ccagcaccag ccagcggcgt gccaggcgcg acagggcacg gaacccgacg
     1621 agcgcgagtc cgacgccgac cacccccagg accgcggcgc cggcccactg ccggctgacg
     1681 ccgagcacca ccgctaccgg cagcggcacc gcggtcccgg cccagaccgc ctcgatcggg
     1741 ccgaccagca gggcggtcgg cggtcgcagc gccacccggt gctcgtcgcc gtaggacgat
     1801 ccgttgacga agtcgctgcc gacgtcggcc gacatcgaca gcaccgccac cgccgcggcg
     1861 accgccaggc cgatccagcc cagggcgccc ggggtctgcg ccaccgcggc gaccgccccg
     1921 gccaggagcg gaagcggtgc caccacccgc agcgccacca gggcgtcggg gagcaacacg
     1981 aacagggcga acagtccgag cacccagacc gcccatgccg cgaccgcgac gccgagcgct
     2041 gtgtcggccc ccgcggtgct ggcctcggcg gcggcgtcgc cgagcagggc gccgagggtg
     2101 aacgggagca ggacccacag ggcgcgggtg atccaggtga ggggggtgac gtgcacggtc
     2161 gtacagactt gcgcgcttcg cgggcccacc gctagcaacg gtggacatgg cggaaggagc
     2221 cgatctgccc ttcgggctgg cttcagcggt cggcccggtg cccttcgcgt cggccgacga
     2281 cgcggtcgcc ttcgcgctcc gccggccccg gtacccgacc gtcccggtca cctcggaccc
     2341 gtccgcttct ctctgggctc aggccgtcag cgggctcgac gacggcgtcg gcgccgatgc
     2401 cgccggcgcg gacctgtcgg gcccggcgtt cgctgcggcc cgcgccttcg tggaagcgct
     2461 cgcccacctc gatgtcgcga gcagcccggt ggcgatccgg gtgccggtgc tgggcccggt
     2521 caccgtggcc tcggagctgc gccgactcgg ccgccccgcc gaggacgccg atcggatcgc
     2581 ggtggaggtc gtgtcggccc gggcggtggc gttgccgggc ctgatccgcg ccgccggcgc
     2641 agtgcccacc tcggcggcag ccccggtggt gtcggtggtg ctcgaggaac cggcgttggt
     2701 cggatccatg catccgacct tcccgcgtcg cccggccgag atccgtgcgc tgctcgatcc
     2761 ggtcatcgac gccctggacc gggccgcgcc tcctcgttcg ctgctcatcg gcgtgcacgt
     2821 cgccggcccc tgcgactggc cgtcggtgat cggctccggg gcgtcgctgc tgtgcgtccc
     2881 ggtcgatcgc accgtctcgg ggtgggcgcc gctgttcggc gacctgctcg accgcggcgg
     2941 acggatctgc tggggtgtcg tgcccgtcga ccggccgctc ggccgctcga ccgaccgcta
     3001 ctggcgtcgc ctggtcgccc tgtggaccgc gatggtcgcc gagggggtcg acccgatggc
     3061 gttgcggctg cggtcgtcgt tctcacccgc ggacggtctc gacgccttcg ggccgtcgca
     3121 ggcggagctg gtcatggagc tgaccgccga ggtggccgaa cgggtcagcc accaggccgt
     3181 ggcggcccgt ctgtcgctcg gcgcctgacc cgggtccgca gcccgtcggg gtgcccgggt
     3241 agggtgccgg accgtgaccg ccgctgggag caccgccgat ccggaccggg atcgctcggc
     3301 ggacggcgag atggacccgg tgggacgcat cgacgacctg cgcgagcaga tccgcggtca
     3361 cgaccgtcgc tactaccagc tcgacgcccc gaccatcccc gacgccgact acgacgcgct
     3421 ggtccgcgag ctgcgctccc tcgaggtcca gcaccccgac ctgatcaccc ccgacagccc
     3481 cacgcagacc gtcggcgggc ccgtgctcac ccagttctcg ccggtgaccc acgccgtgcg
     3541 gatgatgagc ctcgacaacg ccatggaccg cgacgagctg cgggcgtggg gggagcggac
     3601 cgaccggcgc ctcgcctcgc tcggtctcga cggcacggtg cgctacgtct gcgagctcaa
     3661 gatcgacggc ctggccatgt cgctccgcta cgaggacggg gacctggtcc aggccgccac
     3721 ccggggcgac ggccgaaccg gtgaggacgt caccgccaac gtgtcgacca tctccgacat
     3781 ccccacccgc ctcggggccc acgccccgac ggtgctcgag gtccgcggcg aggtctacct
     3841 gccgctcgag gccttccgtc gcctgcagga gcagaccgtc gccgacaacg cggcggccga
     3901 cgcggccggt cgcaagggtc gtccggtgcc ggtcaacccc cgcaacgccg gggccgggtc
     3961 actgcgccag aaggatccga cggtcaccgc gtcgcggggc ctgtcgttct gggcctacca
     4021 gctcggtcag gtcgtcggcg ccgacacgcc ccccacccac tcggccgcgc tcgactggct
     4081 gcgcaccctc gggttgccgg tcaaccccga gacccgcatc ctcgactcgc tcgacgaggt
     4141 ctacgacttc tgcgaccact gggtcgagca ccgccacgac ctgccctacg agatcgacgg
     4201 ggtcgtcgtg aaagtcgacg acctgtcggt gcagagcgcc ctcggctcca cgtccaaggc
     4261 gccgcggtgg gccatcgcct acaagctccc gcccgaggag cgcaccacca cccttctcga
     4321 catccaggtc tcgatagggc gcaccggcaa ggccacgccg ttcgcggtgc tcgagccggt
     4381 cttcgtcggc ggctccaccg tcgggctcgc caccctccac aaccaggacc aggtccgcct
     4441 caaggacgtc cgccccggcg acacggtgat cgtgcgcaag gccggcgacg tcatccccga
     4501 ggtggtcggc ccggtgttgg agctccgtcc cgacggcctg gccgagtggg ccttcccgac
     4561 cgagtgcccc tcgtgcggtg gaccgctggt gcgttccgag ggagaggccc agacccgctg
     4621 cgtcaacccc gactgcccgc agaaggtgct ggcccgcatc gcccacttcg ggtcgcgggg
     4681 cgccatggac atcgagggcc tgggcgagca gcaggtgcag ctgttcctgg agctcggcct
     4741 gctgcacgac gtcgcggacg tctactcgct cgactacgag cgcatccgcg agctgccccg
     4801 ctacggcgac acgtcggtgc gcaacctgag agccgcggtc gaggcgtcga agagccgtcc
     4861 gttggccaac ctgctgttcg ggctcaacat cacccacctg ggccaggccg ggggagaggc
     4921 gctggccgag gggctgggga gcctcccggc gatcatggac gcgtcggtgg acgacctggc
     4981 cgcggtcgac ggggtcgggc cggtggtcgc ggcgtcgatc cacgacttct tcggcgaccc
     5041 cgccaaccgc cggctggttc agcggctgat cgacgccggg gtcaacatcc agggtccgga
     5101 gcggtcggta ctgccccaga cgctgagcgg ccgcagcgtg gtggtgaccg ggtccgtccc
     5161 cggccacagc cgagagagcg ccgaggccgc cgtcaaggcc cgcgggggca agtcgcccgg
     5221 cagcgtgtcc aagaagacct tcgcggtggt cgtcggcgac gaccccgggg cgtccaaggt
     5281 caccaaggcc gagtcgctgg gggtgccgat ggtcagcgcg gcgctgttcg aggagctgtt
     5341 ggagaccggg gagatcccga ccggaggtgg atcgtgacga tgcaggcagt ggtgttcgac
     5401 ttcggaggtg tgttcatcga ctcgcccttc gacggcgtgg ccgccgcggc gcgccgccac
     5461 gggttcgacc cccaggagct gtccgacatc gtgttcggcc cctacgacca ggacaccgac
     5521 cacccgtggc accgcctcga gcggggtgag atctccgtgt cggacgcccg cgacgcgatc
     5581 gtcgtggcgt cggtcgccga cggcggcacc gaggtcgacc cgttcgtcgt cctcgcgggc
     5641 atcgccggag gtgaggtccg cgacgagatg gtcgccttct gccgggaggt ccggagcgcg
     5701 ggactgcgca cgggcctcct caccaacaac gccaaggagt tcgagaccta ctggcgaccg
     5761 ctgctcccgc tcgacgagct gttcgacgac gtcgtcgact cgtccgcggt cggcctgcgc
     5821 aagcccgatc cccgcatcta cgagctgtcg ctgtcgcgcc tcggggtcac cgcagccgac
     5881 gcggtgttca tcgacgacgc ccccggcaac gtcgacggcg cccgggcggt gggcatgagc
     5941 gcggtgctga tcggccagca gcgggccgac gtccccgccg cccttgccga gctccgatcg
     6001 ctcgtcgcgc tgtagcgcag gtctccggaa cctcaggtca cctcgaagca ccagttggtg
     6061 ggcttggcgg tcttcgggcc gtcggtgatc ttccacacca tggccgtcac gcagttgcgc
     6121 ccgctgggca gcgactcgac cggtcgaccc tctccagggg tgtactggac gatccccttg
     6181 ttgggctggc ggatcagtcc gtcggcctgc gtcttgatct ccttgccgtc gatgatcagg
     6241 taggcgtcgt agccctcggc gacgtcgacg ccgaccgtcg actggctgag gatgtggctg
     6301 cccgacgtgg gctccaggcg gtcgacgtac tcggggagca cctcggaggt gcggtcgccg
     6361 ccggtgctgg ccagcatgac ggcgaccacg atgccgacga gtgacccgac gatcagcagt
     6421 gccatgatcg agtacgccca gcggtcgggg agttcgcggc gctccttgcg ggcaggggaa
     6481 gccgccgcgg tcgggccggc ggtcagctcg gcggggtcgt catcggcgtg atcgccgtcg
     6541 gaggggggat cgtcgtgcat gcgggctctc gttctagtcc ccgccgaggg tcccgagccc
     6601 gtcgccgtca ggagtcactc aggcggctcc ccgggtggct cccggtcggc cgataccatc
     6661 gggaagcgtg tcgagccctc ccacgagcag ccaccgcgcc atcgcggaga tcggacggga
     6721 gctgggcggg cggtaccgcc tggtcgcccc gatcggagtc ggcacctcgt cgcgcgtctt
     6781 cctggccgtc gacacccagt tgcgacgccg ggtggcggtc aagctcctcc acgccgcgct
     6841 ggccgcggat gacacgttcc tgcggcggtt ccgcgccgaa gctcgggccg ccggggcgct
     6901 caaccacccc aacatcgtcg ccgtgttcga ctggggcgag gacgagaccg acgaggggat
     6961 gcagccctac ctggtcaccg agtacctggg cggcgggagc ctgcgacgaa tcctcgaccg
     7021 gggtcggccg ctcagcccca gccaggcgct gatggtcggc ctcgacaccg cccgcgccct
     7081 ggcccacgcg catcggcgcg ggctggtgca ccgcgacgtc aagcccggga acctgctgtt
     7141 cgacaccgag ggccgcctga ggctggccga cttcggtgtg gcccgggcgc tggccgaagc
     7201 ggcgtggacc gagccgagcg gaaccatgct cggcaccgcc cgctacgcgt cgccggagca
     7261 ggcgatgggc cgtcgcctcg acggccgctc cgacgtctac agcctggcgt tggtgctcat
     7321 cgagtgcgtc acctgccagg tgccgttcgc cgcggacacg accaccggca cgttggcgtt
     7381 gcggacccag ggagacctcg aggtccccga tgggctcgcc gggttgcggg gcaccctgga
     7441 gcgggccggc cggctcgaac ccgaccagcg tcccgacgcc gaagaggtcg agatctccct
     7501 gctcgcggcg gccgagacca tgccccggcc cgaaccgctg ccgctggtcg ccaccctcga
     7561 cgaagccgag atcaccgccg agctgcgcct ggtcgagggg atcgacgggc tcggcgtcgt
     7621 ctcggccctc gacgcggatg agcccccggt tcccgacgat gccgtcaccg gacgcgccga
     7681 ggacgtcgat cggaccgtcg atgagcccga ggtcctggag cggaccccga tcgtggtcgc
     7741 cgaggagcat cacctcctgg ccacgtcccg accggcgtcg ttcggtcggg tcgagccgtt
     7801 cgtggccgat gcacgggtgg gcggcgatcc gggtgccgcg gatccggggg tggccccgat
     7861 cggcccgatg ccgagcgccc gtcggcgccg gcgagctccg cgcctgctgc tcggggtcgt
     7921 ggccgccctg gcgctgctcg gagcagcggc cgcgtggtgg ttcctgatcc gggtgccggt
     7981 ccacgacgtc cccgacctgg tcggtcgtcc cctcgccgag gcgtccgccc tggcccgcca
     8041 gaacggctgg aagctcgacg acgacacggt cgtgcgggtc gacgacaccc ggccgggtca
     8101 ggtggtcgag cagtcgcccc ggagcgggtc gacgctgtcc gaaggtgaca cgctgcacgt
     8161 cacggtgtcg ctcggaccga ccctggtggc cgtgcccgac ctcaagggcg tacccgaggc
     8221 ggaggcccgg gcccagatcg aggccgctgg gctgaccgcg ggaccgtcga gcaccaccac
     8281 cgacgaggag gttcccgtcg gggcggtcgt ttcggccggt ccggctccgg gccaggaggc
     8341 ccctgcctcg gacggaaccg tgccgcgggg gaccgagctc gctctggtgg tgtcgagcgg
     8401 accggccccc cgggtcgtgc ccgacggcct ggtcggcctg tccgtcgcgg acgccaaggc
     8461 ccgtctggcc gcggtgcagc tgggcgccgc ggtgaccgag gtcaacgacg aggtcgcccc
     8521 ggcggggatg gtcatggcgg tggggacgcc accggggacg tcggtgccgc gtgacaccgc
     8581 cgtcgccctg accgtctcca agggcccggc acccattccg gttcccgacg tggtcggcct
     8641 ggccggcacc gctgcctcgg cgcagatcca ggcggtcggc ctgggcgtgt cgggcatcga
     8701 gggttcgccc tccaacgcgg tgctcgccac cgatccgccc gccggcgagg ccatcccccg
     8761 gggcgccccc gtgcgcatct tcacccgtcg ctgaccgagg gtccaccacc tggcgcgtcg
     8821 gtgcgtttac gttgagcgtc aacaagaccg gatcgacaca caccggatcc gttggggtgg
     8881 tcagccgtac gtggtgttcc ccagccgccg cgagtggcgg ccaccccaac cgatccggtg
     8941 tggcgcgtcc ggggtagcgt cgccgagcca tggtggaccc tccccagccg caggaccccc
     9001 ttcacgatcc gatcaccgac gtcgccgtcg aagcccccgg ggtcgacgag gaggcggtcg
     9061 tcccgtggtc gatgctgttg cgtcgccgtc ccacgggtcc cgccgagagc gggagcggat
     9121 cggaccgacc gtggctggtc acctggaccg tcctgttcgg ggcgttctgg gtgtcgagca
     9181 ccatcacgat cctggcggtc agccgccccg agatcgccca ggacctgggc gcgtcggtcg
     9241 agtcgctggt ctggctgatc tccggaccga ccatcgcggt cgccctcacc ggcaccaccg
     9301 ccgggaagct cggcgacctg cacggccacc gacgggtgta cctgatcggc atggtggggg
     9361 ccacggtgtt cgtcgcgctc accgccctgg cgtggtccgg gccgtcgctg atcgcgttcc
     9421 gggtgctcgg cgccaccatc ggcgccgcca ccgggcccag ctcgctggcg ctgatcaacc
     9481 ggatgttcac cccggaacgc cggtcccggg ctctcggctt ctggtcgctg gtcgtggccg
     9541 ggggaccggt cgtcgggctc gtcgcgggtg gaccgctggt cgaggcgttc ggctggcggg
     9601 tgatcttcgc cgtgcaggcg cccctgctcg cggtggccac cgtcgtcgcg ttcgtcgtgc
     9661 tgcccgagac cggccgacgg cgcgacgtgc acttcgacgt ccccggccag ctgtggctca
     9721 ccgtgaccct cggtggcctg ctgttcggca tcgaccgggc cgcggcatgg gggttgacca
     9781 acccgtggac gatcggcttc ttcctgctgg tgccggtcgg gttcctgtcg ttcctggccc
     9841 gggaacggcg ggccgaacac ccgctgatcc ccctggactg gttcgctcgc cgcagcttct
     9901 cggtcccggt cggcgtgtcg ttcttcatgc agttcggcta catgggcggc ttcatcctca
     9961 ccccactgct gctcgaacag gtgcgggacc tgccggcgga tgtggtcgcg ctcatcatgg
    10021 tgccccgtcc gctcaccttc gccgtcgccg gcccgttggc cggcttcatg gtcgggaggg
    10081 tgtcagcccg ctcgacgatc gtggccggaa gcgcctctct cgcgttgtcg atggtcgtgt
    10141 tcgccctggt ggccgaggac ccgaccaccg ccgtggtcgt cctggccctg accctgtcgg
    10201 gcatcggggt gggagccgcc cagccggcga tcgcctcgtc ggtggccaac tcggtgccca
    10261 accgcgacct ggggatcgcc ggggcgaccc agcagctggt gtcgcaggtg ggcacgacgc
    10321 tcggcatgaa cgtgctcgag gcggtcgaat cggtgtcgag ggacggccgg ggccccggcg
    10381 ggtcctactc gatcgcctac gcggtcgggg ccgcggtcgc cggggcgggc gtggtgctgt
    10441 cgctgttcat gacccggggc cctgacgcgg accggcaccc gaccaccacc cctgacgggt
    10501 gacggctcgg gtgccggcgg ccgcggttcg ggtcgggcga cgaggggcta ctcggtgatc
    10561 tcctggatgg ccgccgcgac cttggccgcg gtctcgtcct ggccgcccac gggctgctgc
    10621 atggccatca gcttggtcat gtcgccctgg accttgacct tgccggccat gaaggcctgc
    10681 atgcccgcct gcgggttgcc ctcgacgatg atcgccttgg cggtcacata gtcgatggtg
    10741 acggtcaggt cggggctctc caggtggccg aggtccatct tgacctcgcc gctggtggtg
    10801 tccatgtggg cctcgaccgt gccgtcgccg aaggggacct cggtgatgac ctggttcatc
    10861 cgcacgacgt gcggtgtggc ggtggcgtcg gagtcgaact gctcgcggat cttcttggcc
    10921 tcctcgatcc actccggcgt gaggaactgg tacttgggca cgacgccccc cttgatcgtt
    10981 ggactgactc gaccggttct ccggcgctgg cagcgtagcg gtgacgctgc tcagcgggaa
    11041 tagacgatcc cgcggagcgg ccgccggcgc ccggtaccgt caagcactgt gagccatccc
    11101 gaccccacgt ggcctgtgtc acaccccccg accaccccgc cgccgccccc gccgccgggc
    11161 tacggcagcc cgccgccggg ctacggcacc ccgccgccac ctcccgccgg cggtggctcc
    11221 ggacgagggg ccaaggtggt
//
LOCUS       DAOQOD010000101        11184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2074_length_11184_cov_10.913919, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000101 DAOQOD010000000
VERSION     DAOQOD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_11184_cov_10.913919"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLZ72_10460"
     CDS             complement(<1..898)
                     /locus_tag="PLZ72_10460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA36761.1"
                     /translation="MSTPAPVAGEAPAPAAAPRAFGLDVLRVLAAVGVLVTHVAFATG
                     VVNPQRWSSPLRHVLPRLDVGVWIFFVLSGLFICRPYVRAALGGTEPAPTRTYALRRL
                     SRVYPLYWFVLGVSVLTAAGPRPPSGQLLADVLLLNVYVPAWAIGPITQAWSLSTELA
                     FYAFVPVWFWGCRRLFASRSITDPATRTRWLAGGLVGWAAVALLWRVGVIAVTDTFDY
                     TVAGAVDTRGALLTWLPNHLDTFAIGAAFALWLESGRARALSTAARLGCYALAAGALA
                     AASGALGLPPLFTGFDGPQTLGR"
     gene            complement(895..2073)
                     /locus_tag="PLZ72_10465"
     CDS             complement(895..2073)
                     /locus_tag="PLZ72_10465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA36762.1"
                     /translation="MVTAAVERTRATRFPYIRSLDGLRGLLVFPVAAFHFSLTAATDP
                     ADRHILAPGAFFAPSAFFALSGFLITSLLMVEHERTGSIDGRGFWARRFRRLLPASVF
                     VVLVVVAVGAVLPQWWGPLPVPDIASALFSVQNWWSIHLANTDQAYRLLGPLGVYWSL
                     AVEEQFYLGLFLVVFVASRTRHMIRWLTGALVTIGTLSIISLVVVQSSPQREFFGTDS
                     RASELVAGCLVAVAFHHWGWPKGRWWGWAAWIALAATALAWLFVWEDDSFVLGGALAL
                     ISIPNVFLICGATVEGGFQRFLGLRPLVELGRISYPVYLVHWPIGLVMNPVRMGFGGL
                     PLIVARFAVSVAVGYGITRLLEKPLRTSSLVRWPRGIVVWGTAVVAAIGVSWATAGWS
                     "
     gene            2161..3411
                     /locus_tag="PLZ72_10470"
     CDS             2161..3411
                     /locus_tag="PLZ72_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36763.1"
                     /translation="MQLADRSPAPPVTGVHEGFRLLGWWCAGVVVAVVHNGLWATPNL
                     EAMSQVARHLGSNPFSGPNAPDYVMTDVLVPVVSRAVGQSGPAAYARLHLVVLVVLWA
                     VVAAVARSRFGYRSARNLVVLLAVSPLVTVSMQWLGQPDPLTATLGILMVLLRPRWAV
                     LVVAVLAGLTHPEQAVFMAAVAAVVRAVLVDDVAVADAAAPASGSSLRTRWRPVGLDL
                     AASVGGVLLGRLVTEVYFRVAGIELGRPRTAYLDFGLGRLVEHHLQQPLGLLWTLWGP
                     LWLVVAGVICVRVLRRGVDPGSARRWAVLATAAVLALVPVLVTLDETRVYAVITAPLL
                     VGVAVLLDRERPPLPDRLVAGFAVGLLGLSLVVPGGFATGVTSWRGQLDTPAMVEFLS
                     DGTRPPGDLTPWLLSPFDFTIPAL"
     gene            3518..8095
                     /locus_tag="PLZ72_10475"
     CDS             3518..8095
                     /locus_tag="PLZ72_10475"
                     /inference="COORDINATES: protein motif:HMM:NF023275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HNA36764.1"
                     /translation="MDAAPAADRPRRRPALGAVLLVVVAYVPLLLTKPGRIGADTKTY
                     LYLDPARLLSRAVSMWDPNIGLGTITHQNIGYLWPMGPYYWLMQTVGVPDWIAQRLWL
                     GTIIAAAGLGVRFLLRELNWRSSGVTVASFAYALSPYLLDYGARISVILLPFAGLPWL
                     VALAARSVRRGGWRDPALFALVTLTVGGVNATSLLLVMVAPLLWMAHATFVARESTLG
                     HTVAAGLRISVLTLITSFWWIAGLAVQGAFGIPILRYTETYYVVANAALAPELLRGLG
                     YWYFYGRDALGPWIRPAISMVQSVPLLAVSYAIPILAFCGGLLSRFRHRGYFTLLVLV
                     GLVMGVGAHPWTGSSPAGAVFKLWTTTDSGLAFRSTPRAVPLIALGLAVLLGAGAAAV
                     GRWRPGLRVPVAAGLLVLICLNQLALFRGQMVDENLDRPSDVPTYWTDAAAFLDRGDP
                     DYRVAEVPGVDFATYRWGNTVDPITPGLMDRDYVARELIPYGTPASADMLNDWDLPLQ
                     DGTVDPATIAPIARLLGVGQIVHRADLQYERFRTPRPRWLNSVLADAPGLGEPVTFGA
                     ARANDPNPLLPLDDAIEYGQPASDRDPAPVSVFPVEDPRPMLRTVQASAPVLMAGNGA
                     GIVGLARSGGLQVDRPLFYSASFTTGTDGTNDSDTSKSDSETDRISGIAAEPGASLVV
                     TDTNRRQARRWGSVRENDGYTEMAGETPLVKDPADNRLDVFPGAADDAYTVTEQVGGA
                     VARASSYGNPVSYTASGRAVRAVDGDPTTAWQVGAFEGVDGEFLQLTFPEPVTTDRIT
                     LLQAQKPANRWITSVELSFDRSERAVDDPDAGAPLGGASRPVALGDASRQAPGQEIRF
                     PSRTFRTLRITITGTNLGDLPRWTGVSDVGIAEIAVPGVAPTTEVVRPPTDLLDALGA
                     RSTARPLSYVFQRRAADPAEVVLDDEERRMQRWVEGPTLRGFLPFGTARISGRIPGER
                     VDALIGAPDAAHGGLTATAAATLAGDLRSRPSVAVDGDDATAWQTPVNGAVGDWIEVT
                     YPAPVTVDDLTMAIVADGRHSVPTKVSLQVDGADGPTLDLAAAGVSSLAHPDGTVDRT
                     GARGATTAVRVPTGSVTGTTFRFRVDEVAEVRSLDWFSGTPTVLPVGIAELGLPTRAA
                     LADDSRLPAACRTDLLTIGGQPVGLRVLGTVGQALDRAELRLEWCADHSVPGPGVSIP
                     AGRTLLETADGCSTGIDVDMLTLASAAGGGTGTDTLAEPPGPGPTPPSTTTDRPGRIT
                     YTAKVTDADQPYWVVLGQSLSPGWTATAEGSRGSADLGEPTLVNGYANGWQVDPSVVG
                     ADATITMTFAPQRMVWMAIWASLLGVAVCLVLALVPHRWWARLPWRRGRRSGAPGEGP
                     GASEGSRGQRRAEGPADAAAVADVEVEAAGPLLVDGVSLSAGRSLALGLAVGVVAAVF
                     LGPWVGVATAAVTAVALGLRRGQVLLRAACVGAFGLAAAFIVAKQLRADYVVDFEWMN
                     NFEITHAWALFAAGLLAVDPLVEVLRRRRRS"
     gene            complement(8253..9035)
                     /locus_tag="PLZ72_10480"
     CDS             complement(8253..9035)
                     /locus_tag="PLZ72_10480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA36765.1"
                     /translation="MKLTGERPMQGQTPDSLLALHTAGYREVRARMGDGRFLDVGCGL
                     GDESIGFAGEGRELLGVDYDPETAATAVRTHGAAGMNAICSDGSLLALRSDTFDDVCS
                     SHLIEHFTEPEHHVSEVARVLSSDGTAFFITPNEPADFENPYHVYLFGPDELRDMLGR
                     HFGTVEMHGLDATPRVKADFEKRREMANKLLKLDPLGLRHKLPREAFVWLHATGRRLA
                     YRFTNTEQVGGASGITDDEFFLTDDIDDSTLVLFAVCSDPID"
     gene            9175..10464
                     /locus_tag="PLZ72_10485"
     CDS             9175..10464
                     /locus_tag="PLZ72_10485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA36766.1"
                     /translation="MPEPVTPDPPVGSAVDAIAEMAGRVGIERIHVFGWRDLDDDEAG
                     GSEVHADSIESIWARAGLQVTHRTSFSSGRPPIDVRNGYRVSRRGNRYLVFPRAAVAE
                     LTGRLGPTDAVIEIWNGVPFLSPWWWHGPTSVWLHHVHGPMWQQSLPGPVAPLGNLLE
                     ERLAPPWYRRVPVVTLSQSSKDELVDELGFDRGHVTVIPPGIDPSFSPLDPADPGVGR
                     SPTPLAVAVGRLTPVKDFCRLVRVMARVRDRVPDLELVIVGEGYERAAIEAQVAAVGG
                     QDWVRLPGRISDEELLSLYRRAWLAVSASTREGWGMTLTEAAACGTPAVATDIAGHAD
                     AIDDGRSGLLGRSDDELVELATRVLTDATTRSALRAGALERAGELTWEACALANFRVL
                     ADDAAALAAVGGRRRRLPKHPRGVPTGAPRRRAGVGE"
     gene            10481..11140
                     /locus_tag="PLZ72_10490"
     CDS             10481..11140
                     /locus_tag="PLZ72_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36767.1"
                     /translation="MTDGVGDFVLGVDLDGVCADYTEAFAQVVAAERGVPIDSLTREV
                     SWDFAEWDLDREEFLRLHRLGVQERHMFATMPALDGVADALWRLSDAGVWIRIITHRL
                     VTNWGHARIVSDTVEWLDTNRIPYRDLCFLGAKPEAQADAYVEDAPHNVAALRAGGNT
                     VVVFDQPYNRELDGPRAANWAEAEAVIAELVAAHTGAFSPQLPGVEAGADRLDRHITA
                     L"
BASE COUNT         1496 a         4044 c         4062 g         1582 t
ORIGIN      
        1 ggcggcccaa cgtctgcggt ccgtcgaagc cggtgaacaa cggcggaagc cccagcgcac
       61 ccgacgcggc cgccagcgcc ccggcggcca gcgcgtagca cccgagccgc gcggcggtgg
      121 acagcgcccg ggcccgtccg gactccagcc agagcgcgaa cgcggcgccg atcgcgaagg
      181 tgtcgaggtg gttcggcagc cacgtcagca gcgccccacg tgtgtcgacg gcgcccgcga
      241 cggtgtagtc gaaggtgtcg gtgaccgcga tcacgcccac ccgccagagc agagccaccg
      301 ccgcccagcc caccagcccg ccggccaacc acctcgtccg agtcgcgggg tcggtgatcg
      361 accgggacgc gaacagccgg cggcaccccc agaaccacac gggcacgaac gcgtagaagg
      421 cgagctcggt cgacaggctc cacgcctggg tgatcgggcc gatcgcccag gccggcacgt
      481 acacgttgag caggaggacg tcagcgagga gctgtccgct cggcgggcgc ggcccggctg
      541 cggtcaggac cgacaccccg aggacgaacc agtacagcgg gtacaccctc gacagccgcc
      601 gcagggcata ggtgcgcgtc ggtgcaggtt cggtcccgcc gagcgcggcg cgcacgtacg
      661 ggcggcagat gaacagcccc gacagcacga agaagatcca gacgccgacg tcgaggcggg
      721 gcagcacgtg gcgcagcggc gacgaccacc gctgcgggtt gaccaccccg gtggcgaacg
      781 cgacgtgcgt gacgagcacg ccgaccgcag ccagcacgcg cagcacgtcc aggccgaagg
      841 cccggggggc ggctgcgggc gccggggcct cgcccgcgac gggtgcaggt gtgctcacga
      901 ccacccggcc gtcgcccacg acaccccgat cgcggcgacc accgcggtgc cccacacgac
      961 gatcccccgc ggccaccgca ccagcgacga cgtccgcagc ggcttctcga gcagccgggt
     1021 gatcccgtag ccgacggcga ccgacaccgc gaaccgggcg acgatcagcg gtaggccgcc
     1081 gaagcccatg cgcaccgggt tcatcaccag accgatcggc cagtggacca ggtacaccgg
     1141 gtagctgatg cgcccgagct cgaccagggg gcgcaggccg aggaaccgct ggaacccgcc
     1201 ctcgaccgtc gcgccgcaga tcaggaacac gttggggatc gagatcagcg ccagggctcc
     1261 gccgagcacg aacgagtcgt cctcccacac gaacagccac gccagggcgg tggcggccag
     1321 ggcgatccac gccgcccacc cccaccagcg tcccttgggc cagccccagt ggtggaacgc
     1381 gacggcgacc aggcagccgg cgaccagctc cgaggcccgg ctgtcggtgc cgaagaactc
     1441 gcgctgaggc gaggactgca cgacgacgag ggagatgatc gacagggtgc cgatggtgac
     1501 gagcgctccg gtcagccagc ggatcatgtg gcgggtgcgc gacgccacga acaccaccag
     1561 gaacaggccg aggtagaact gctcctcgac cgccagggac cagtagacgc cgaggggacc
     1621 gagcagtcgg taggcctggt cggtgttggc caggtggatc gaccaccagt tctggacgct
     1681 gaacagcgcg gacgcgatgt cggggaccgg cagcggaccc caccactggg gcagcaccgc
     1741 cccgaccgcc accacgacga ggacgacgaa gaccgacgcc ggcagcaggc gacggaagcg
     1801 acgggcccag aagccgcggc cgtcgatcga tccggtgcgc tcgtgctcga ccatcagcag
     1861 agacgtgatc aggaacccgg acagggcgaa gaaggcggag ggggcgaaga acgcccccgg
     1921 agcgaggatg tggcggtcgg cggggtccgt ggccgcggtc agcgagaagt ggaacgcggc
     1981 gaccgggaac accagcaggc cgcgcagccc gtcgagcgag cggatgtagg ggaacctggt
     2041 cgcccgtgtg cgctcgacgg cggccgtgac catgccgggg agcatacggg tcgacccgcg
     2101 ggcgcccggt gacctgggag agccccgtcg gcggccgacc gcaccgctac ggtcgccgcc
     2161 gtgcagctcg ccgaccggtc tcccgcgccg cccgtcaccg gcgtccacga ggggttccgg
     2221 ctgctggggt ggtggtgcgc gggcgtggtc gtggcggtgg tgcacaacgg gctgtgggcg
     2281 accccgaacc tcgaggcgat gtcgcaggtg gcgcggcacc tgggctccaa cccgttctcc
     2341 ggtccgaacg ccccggacta cgtgatgacc gatgtgctgg tcccggtcgt gtcgcgggct
     2401 gtcggccaga gcgggccggc cgcctacgcc cggctgcacc tggtggtgct ggtcgttctc
     2461 tgggccgtcg tcgccgccgt ggcccggagc cggttcggct accgcagcgc ccggaacctg
     2521 gtcgtgctgc tcgcggtgtc accgctggtg accgtctcga tgcagtggct gggtcagccc
     2581 gacccgctca ccgccaccct cgggatcctg atggtcctgc tgcggccacg ctgggcggtg
     2641 ctcgtcgtgg cggtgctcgc cgggctgacc caccccgagc aggcggtctt catggcggcg
     2701 gtggcagcgg tggtccgggc ggtgctcgtc gacgacgtcg ctgtcgccga cgccgcggcg
     2761 ccggcctccg ggtcgtcgct ccggacgcgg tggcgtccgg tcgggctcga cctggcggcc
     2821 tcggtcggcg gggtgctcct cggccgcctg gtcaccgagg tctacttccg ggtggcgggc
     2881 atcgagttgg gtcgaccgcg cacggcctat ctcgatttcg gcctcgggcg cttggtcgag
     2941 caccacctcc agcaaccgct cgggttgctg tggaccctgt ggggtcccct gtggctggtc
     3001 gtggccggcg tgatctgcgt gcgcgtgctg cgacgtggcg tcgaccccgg ttccgcccgt
     3061 cggtgggcgg tgctggccac ggccgcggtc ctggcgctgg tgcccgtgct ggtcaccctc
     3121 gacgagacga gggtctacgc ggtgatcacc gcaccgctgc tggtcggggt cgccgtgctg
     3181 ctcgaccggg agcgtccgcc gctgccggat cggctcgtcg ccgggttcgc cgtcggcctg
     3241 ctgggcctgt cgctggtggt tcccggaggg ttcgccaccg gggtgacgtc gtggcggggt
     3301 cagctcgaca cgccggccat ggtcgagttc ctgagcgacg gcacccggcc gcccggcgac
     3361 ctgaccccgt ggctgctgtc gccgttcgac ttcaccatcc cggcgctgtg agggcgccgg
     3421 ctccctgacc ggccgcaccc ggttctgacc cggccgctct gccggtcccc ggtggcagtc
     3481 cacggccggg gcctcgggct agcgtcaacg cccgaccatg gatgccgccc ccgcagccga
     3541 ccggccccga cggcgcccgg cgctcggcgc ggtcctgctg gtcgtcgtcg cctacgtccc
     3601 gctgctgttg accaagccgg gccgcatcgg cgccgacacc aagacctacc tgtacctgga
     3661 tcctgcccgg ctgctgtcac gggcggtgtc gatgtgggac ccgaacatcg gcctcggaac
     3721 catcacccac cagaacatcg gctacctctg gccgatgggt ccctactact ggctgatgca
     3781 gaccgtcggg gtgcccgact ggatcgcgca acgcctctgg ctggggacga tcatcgcggc
     3841 cgcgggtctg ggggtccggt tcctgctgcg ggagctcaac tggcgatcga gcggggtgac
     3901 cgtggcctcg ttcgcctacg cgctcagccc gtacctgttg gattacggcg cccggatctc
     3961 ggtcatcctg ctcccgttcg ccggtctgcc ctggctggtc gcgctggcag cccggtcggt
     4021 gcgccgcggc ggatggcggg acccggcgct gttcgccctc gtgaccctca ccgtcggagg
     4081 ggtcaacgcc acgtcgctgc tcctggtcat ggtcgccccg ctgctgtgga tggcgcacgc
     4141 caccttcgtc gcccgcgagt cgacgttggg tcacacggtg gcggcagggc tccgcatcag
     4201 cgtcctgacc ctgatcacca gcttctggtg gatcgccggc ctggccgtcc agggtgcgtt
     4261 cggcatcccg atcctgcgct acaccgagac ctactacgtc gtcgccaacg ccgccctggc
     4321 ccccgaactg ctccgtgggc tcggctactg gtacttctac ggtcgcgacg ccctcgggcc
     4381 ctggatccgt cccgccatct cgatggtcca gagcgttccg ctgctcgcgg tgagctacgc
     4441 gatccccatc ctggccttct gcggcgggct cctgtcgcgg ttccggcacc ggggttattt
     4501 caccctgctg gtcctcgtcg gtctggtcat gggcgtcggc gcccacccgt ggaccgggtc
     4561 gtcgcccgcc ggggcggtgt tcaagctctg gaccacgacc gactcgggac tggcgttccg
     4621 ctccacaccg agggcggtgc cgctgatcgc cctgggcctg gcggtgctcc tgggcgccgg
     4681 cgccgcggcg gtcggccggt ggcggcccgg gctgcgggtc ccggtggcgg cggggctgct
     4741 ggtgctgatc tgcctcaacc agctcgccct gttccgcggc cagatggtcg acgagaacct
     4801 cgaccggccg agcgacgtgc cgacctactg gaccgacgcc gcggcgttcc tcgaccgggg
     4861 cgatcccgac taccgggtgg ccgaggtccc gggcgtggac ttcgccacct accggtgggg
     4921 caacaccgtc gacccgatca cgccgggact gatggaccgc gactacgtgg cccgggagct
     4981 gatcccctac ggcacgccgg cctcggccga catgctcaac gactgggacc tgccgttgca
     5041 ggacgggacc gtcgacccgg ccacgatcgc tccgatcgcc cggctgctcg gagtcggcca
     5101 gatcgtgcac cgcgccgacc tccagtacga acggttccgc acgccacggc cccgctggtt
     5161 gaactccgtg ctcgccgacg ctccgggtct gggcgagccc gtcacgttcg gtgcggcccg
     5221 ggcgaacgac ccgaacccgc tgctccccct cgacgacgcc atcgagtacg gccagcccgc
     5281 gtcggatcgt gacccggcgc cggtgtcggt gttcccggtc gaggaccccc gcccgatgct
     5341 gcggaccgtg caggcctccg ctccggtcct gatggccggc aacggcgccg ggatcgtcgg
     5401 actggcccgc tccggcggcc tccaggtcga ccggccgctg ttctactcgg cgtcgttcac
     5461 gaccggcacc gacggaacca acgacagcga caccagcaag agcgacagcg agaccgaccg
     5521 gatcagcggg atcgccgctg agccgggtgc gtcgctcgtc gtcaccgaca ccaaccgccg
     5581 ccaggcccga cggtggggtt cggtgcggga gaacgacggc tacaccgaga tggccggcga
     5641 gaccccgctg gtgaaggacc ccgccgacaa ccgtctcgac gtgttccccg gtgccgccga
     5701 cgacgcctac accgtgaccg aacaggtcgg cggggccgtc gcccgggcgt cgtcgtacgg
     5761 caacccggtc agctacaccg ccagcggtcg tgcggtgcgc gccgtcgacg gcgacccgac
     5821 gaccgcctgg caggtgggag cgttcgaggg cgtcgacggc gagttcctcc agctcacctt
     5881 ccccgagccg gtgaccaccg accggatcac cctgctccag gcgcagaagc ccgccaaccg
     5941 ctggatcacc tcggtcgagt tgtccttcga ccgctcggag cgggccgtcg acgaccccga
     6001 cgccggtgcg ccgctcggcg gggcctcccg accggtggca ttgggagacg cctcccgtca
     6061 ggcccccggc caggagatcc ggttccccag ccgcaccttc cgcacgctgc ggatcaccat
     6121 caccggcacc aacctcgggg acctgccccg ctggaccggc gtgtccgacg tcggcatcgc
     6181 cgagatcgcc gtccccggag tcgcaccgac gaccgaggtc gtccgacctc cgaccgacct
     6241 gctcgacgct ctgggcgccc ggtcgacggc gcgtccgctg tcctacgtgt tccaacgccg
     6301 ggcggccgac ccggccgagg tggtcctcga cgacgaggaa cgccggatgc agcggtgggt
     6361 cgaggggccg acgctccgcg gcttcctgcc gttcggcacg gcccggatct cgggacggat
     6421 ccccggcgag cgggtcgacg cactcatcgg agcgcccgac gcggcacacg gaggcctgac
     6481 cgcgaccgcg gcggcgacac tggccggcga cctccgctcc cggccgtcgg tggcggtcga
     6541 cggcgacgac gccaccgcct ggcagacacc ggtcaacggc gcggtgggcg actggatcga
     6601 ggtcacctac cccgcgccgg tcaccgtcga cgacctgacg atggcgatcg tcgccgatgg
     6661 ccgccactcc gtgccgacga aggtgtcgtt gcaggtggac ggcgcggacg ggcccaccct
     6721 ggacctggcc gccgccggcg tctcgtcgct cgcccatccg gacgggaccg tcgaccgcac
     6781 cggagcgcgg ggcgccacga ccgcggtccg ggtgccgacg ggatcggtca ccggcaccac
     6841 gttccggttc cgcgtcgacg aggtcgccga ggtgcgttcg ctcgactggt tcagcgggac
     6901 accgaccgtg ttgccggtcg gcatcgccga gctcggcctg cccacccggg ccgcactggc
     6961 cgatgactct cggctgccgg cggcgtgccg gaccgatctg ctgacgatcg ggggacagcc
     7021 ggtcgggttg cgggttctcg gcacggtcgg acaggccctc gatcgcgccg agctccgcct
     7081 cgagtggtgc gcggatcact ccgtgcccgg ccccggcgtg tcgatcccgg cgggacggac
     7141 tctgctcgag accgctgacg gctgctcgac cggcatcgac gtcgacatgc tgacgctcgc
     7201 ctcggcggcc gggggcggga ccgggaccga caccctggcc gagcctccgg gcccggggcc
     7261 gacaccgccg tcgaccacca ccgaccggcc ggggcgcatc acctacaccg ccaaggtgac
     7321 cgacgcggac cagccgtact gggtcgtgct gggccagagc ctgtcgcccg ggtggacggc
     7381 gacggccgag ggcagcaggg gttctgcgga cctcggcgag ccgacgctgg tcaacggcta
     7441 cgccaacggc tggcaggtcg acccgtcggt cgtcggcgcc gacgccacga tcaccatgac
     7501 gttcgctccg cagcggatgg tgtggatggc gatctgggca tccctcctcg gcgtggcggt
     7561 gtgcctggtg ctggccctgg tgccgcaccg gtggtgggct cgcctcccgt ggcggcgggg
     7621 tcggcgctcc ggagcacccg gagaggggcc gggagcatcc gagggatcac gcggacagcg
     7681 ccgggcggag gggccggcag acgccgcggc ggtcgcggat gtcgaggtcg aggccgccgg
     7741 gccgctgttg gtcgacggcg tctccctcag cgcagggagg tccctggcgc tcgggctggc
     7801 ggtgggcgtg gtggctgcgg tgttcctcgg cccctgggtc ggcgtggcca ccgccgcggt
     7861 gacggcggtg gcgttggggc tgcgacgcgg tcaggtgctc ctgcgggccg cgtgcgtcgg
     7921 ggcgttcggc ctggctgccg cattcatcgt cgccaagcag ctccgggccg actacgtggt
     7981 cgacttcgag tggatgaaca acttcgagat cacccacgcg tgggcgctgt tcgccgccgg
     8041 tctgctggcc gtcgacccgc tcgtcgaggt gctccgacgt cgacgacgct cctgaagcgc
     8101 cgttcgcgtg tggatccgac ctctgggacg tcggtctcac acgcggacac cgggcgccgg
     8161 ccgttcgcgt gtggatccga cctctgggac gtcggtctca cacgcggaca ccgagaaacc
     8221 caccggattc gtcagcccac gcggggtgcc ggtcagtcga tcgggtcgga gcagaccgcg
     8281 aacagcacca gggtcgagtc gtcgatgtcg tcggtcagga agaactcgtc gtcggtgatg
     8341 cccgacgccc caccgacctg ttcggtgttg gtgaaccggt aggccaggcg tcgcccggtc
     8401 gcgtgcaacc acacgaacgc ctcgcggggc agcttgtggc gcagcccgag cggatcgagc
     8461 ttgagcagct tgttcgccat ctcgcgtcgc ttctcgaagt cggccttgac ccggggggtg
     8521 gcgtcgaggc cgtgcatctc gacggtgccg aagtggcggc cgagcatgtc gcgcagctcg
     8581 tcgggcccga acaggtacac gtggtacggg ttctcgaagt cggcgggctc gttgggtgtg
     8641 atgaagaagg cggtgccgtc ggagctcagg acccgggcga cctccgacac gtggtgctcg
     8701 ggctcggtga agtgctcgat caggtgcgat gagcagacgt cgtcgaaggt gtcggagcga
     8761 agcgccagca gggagccgtc ggagcagatc gcgttcatgc cggcggctcc gtgggtgcgc
     8821 accgcggtgg ccgcggtctc ggggtcgtag tcgacgccga gcagctcacg cccctcgccg
     8881 gcgaagccga tcgactcgtc gcccagcccg cagccgacgt cgaggaaccg gccgtcgccc
     8941 atccgggccc gcacctcgcg gtaccccgcg gtgtgcagtg cgagcagcga gtcgggggtc
     9001 tggccctgca tggggcgctc gccggtcagc ttcacgggga ctccagtcca ggttcgctcc
     9061 ccctggttcc cgttctgtag tgcgagatcc aggcgaacgc ctggatctcg cactacagaa
     9121 cggcggggag ggtgtcggtc gggtggggaa ggtccgcgta gcatacgggt ccccatgcct
     9181 gagcccgtga cccccgaccc gcccgtcggt tccgccgtcg acgcgatcgc cgagatggcc
     9241 ggtcgggtcg gcatcgaacg catccacgtg ttcggttggc gcgacctcga cgacgacgag
     9301 gccggcggct ccgaggtcca cgccgacagc atcgagtcga tctgggcccg ggccggtctg
     9361 caggtcaccc accggacgtc gttctcgtcc ggtcgacctc ccatcgacgt gcgcaacggc
     9421 taccgggtca gccgccgggg gaaccgctac ctggtcttcc cccgcgcggc ggtggccgag
     9481 ctgacgggtc gactcggtcc gaccgatgcg gtcatcgaga tctggaacgg ggtcccgttc
     9541 ctgtcgccgt ggtggtggca cggtcccacc tcggtctggt tgcaccacgt ccacgggccg
     9601 atgtggcagc agagcctgcc cggaccggtg gcgccgctgg gcaacctcct cgaggaacga
     9661 ctcgccccgc cgtggtaccg gcgggtgccg gtggtgactc tgtcgcagtc gtccaaggac
     9721 gagctcgtcg acgagctcgg gttcgaccgt gggcacgtca cggtgatccc gccggggatc
     9781 gacccgtcgt tctcgccgct cgacccggct gacccgggtg tcggccgctc ccccaccccc
     9841 ctggccgtcg ccgtcggccg tctcaccccg gtcaaggact tctgtcgact ggtgcgtgtc
     9901 atggcccggg tccgggatcg ggtgcccgac ctggaactgg tcatcgtcgg tgagggctac
     9961 gagcgcgccg cgatcgaggc ccaggtcgct gcggtcggcg gccaggactg ggtccggctg
    10021 ccgggccgca tctccgacga ggagctcctg tcgctgtacc gccgggcgtg gctggcggtg
    10081 tcggcgtcga cgcgtgaggg ctggggcatg accctcaccg aggccgcggc ctgcggtacc
    10141 ccggcggtgg ccaccgacat cgccggccac gcggatgcca tcgacgacgg ccgcagcgga
    10201 ctgctcggcc gatccgacga cgagctggtg gagctggcga cccgggtgct caccgacgcg
    10261 acgacccgat ctgctctccg ggccggggcg ctcgagcgag ccggcgagct gacctgggag
    10321 gcgtgcgcgc tggccaactt ccgcgtgctg gcagacgatg ccgccgcgct cgccgcggtc
    10381 ggtggacgtc gtcgacgtct gcccaagcac ccgcggggcg ttccgacggg cgcgccgcgc
    10441 cgacgcgctg gagtcggcga gtgaccgacg gagtcggcga gtgaccgacg gagtcggcga
    10501 cttcgtcctc ggggtcgacc tcgacggggt ctgcgccgac tacaccgagg cgttcgccca
    10561 ggtggtcgcc gccgagcggg gcgttccgat cgactccctc acccgcgagg tcagctggga
    10621 cttcgccgag tgggacctcg accgcgagga gttcctccga ctgcaccgcc tgggggtgca
    10681 ggagcgccac atgttcgcga ccatgccggc tctcgacggc gtggccgacg cgctctggcg
    10741 gctgtcggat gccggggtgt ggatccggat catcacgcac cggctggtca ccaactgggg
    10801 tcacgccagg atcgtctccg acaccgtgga gtggctggac accaaccgga tcccgtaccg
    10861 ggacctgtgc ttcctggggg ccaagcccga ggcccaggcc gacgcctacg tggaggacgc
    10921 cccccacaac gtcgccgcgc tgcgggccgg cggcaacacc gtcgtcgtgt tcgaccagcc
    10981 ctacaaccgt gagctcgacg gtccgcgggc ggcgaactgg gccgaggccg aggcggtgat
    11041 cgccgagctg gtcgcggccc acaccggcgc gttctcgccg cagctgccgg gcgtcgaggc
    11101 cggtgccgac cgcctcgacc gccacatcac cgctttgtag tgcgagatcc aggcgttcgc
    11161 ctggatctcg cactacagaa cggg
//
LOCUS       DAOQOD010000102        11095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2096_length_11095_cov_10.660236, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000102 DAOQOD010000000
VERSION     DAOQOD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_11095_cov_10.660236"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLZ72_10495"
     CDS             complement(<1..680)
                     /locus_tag="PLZ72_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HNA36768.1"
                     /translation="MSSTESSPTAPSAPRITPTRGGIALAVVGAVLMGLGIFASTGAA
                     PEPVCPSGTRLVAKFSRLPSTGTYSFVEPAGNAKVVSLSATSATGGTFASAAAISAVV
                     VKAGDGSKDAWITPASTTGTFDNTGIPPASGSIPAVSTVQFCAAASVNPATLAAGFSA
                     VAHGDAALTADGSTGPIAVGGNVSFDRYSVAGHSAGVYKVDPTDSSATALVVRGRVEL
                     ARSAGDLSV"
     gene            923..995
                     /locus_tag="PLZ72_10500"
     tRNA            923..995
                     /locus_tag="PLZ72_10500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:956..958,aa:Arg,seq:cct)
     gene            989..1219
                     /locus_tag="PLZ72_10505"
     CDS             989..1219
                     /locus_tag="PLZ72_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36769.1"
                     /translation="MGAQWIVRSRARVSAAEQRRSTPTFACEIDHREVDPTRGHRALE
                     LQLLDSVVDGSGNRYVHSEGGDSPYRGLIALG"
     gene            complement(1224..2408)
                     /locus_tag="PLZ72_10510"
     CDS             complement(1224..2408)
                     /locus_tag="PLZ72_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNA36770.1"
                     /translation="MTTKTYVIGVGMTRFEKPGSRDWDYPDMGREAGEKALADAGIDY
                     SDIESAFIGYCYGDSTSGQRALYELGLTGIPAVNVNNNCSTGSSALYLGRRFVQGGLA
                     DCVLALGFEKMQKGSLKGNFEDRTNPMDKHFTDMVQRRGFEPAPPAAQLFGNAGREYM
                     ERYGVTAETFARIGEKNHRHSVNNPYSQFRDQYSLQDILDAPTVYDPLTKLQCCPTSD
                     GGAAAVVASERFVKEHGLEDRAVEIAGQAMTTDFDDTFEGSDMAIAGIHLTEAAARQA
                     YEESGLGPEDAQVIELHDCFSANELITYQGLGLCAEGKAGELIHDGAVTYGGRWVVNP
                     SGGLISKGHPLGATGLAQCSEITWQLRGDAEARQVDGARVGIQHNLGLGGAAVVTVYR
                     KD"
     gene            complement(2414..3118)
                     /locus_tag="PLZ72_10515"
     CDS             complement(2414..3118)
                     /locus_tag="PLZ72_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36771.1"
                     /translation="MRPSPRQVARSATAAAAVAVLAILAACSAAPRAPQPQPPSGPGY
                     RLTEWAPSSSFVVRVHPSAGSLTDEVMAAAAEVTARSGITFVLGPPAPTPAPDLVGST
                     PEITVVVGTYCSAGAIGCAEMWGQWGFTPEERHVIRDVRLSVVPAMLDDAATRRAVLL
                     HELGHAVGLDHHSETFEGQHQVMNPYVEPTMSQYRSGDDAGLAATGTRARPATDPAAA
                     LLQLFGATPPAARRLP"
     gene            3356..3748
                     /locus_tag="PLZ72_10520"
     CDS             3356..3748
                     /locus_tag="PLZ72_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNA36772.1"
                     /translation="MALDPDLKDLAQGKNFAALTTLMPDGHPQTQIMWVGADDEHVII
                     NTQLDRQKYRNVLADPRVTVTVFDADNPYRYVEARGRVARTQDGAAAAASIDELSRKY
                     TGGDYGMGPTDSRVILFIDVERVHKNGY"
     gene            complement(3804..5162)
                     /locus_tag="PLZ72_10525"
     CDS             complement(3804..5162)
                     /locus_tag="PLZ72_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNA36773.1"
                     /translation="MLSELVHGVQSGAIDPVDLVEEAVRRIDAGDGAINAVVRLDADA
                     AREVAAGHDRSGPLAGLPFLVKDMARCAGSVTTYGSAMFVDADPDDSDDDAVARLRAA
                     GAIVLGRTNTPAFGHAPFTANLVFGATRNPWNTDCSPGGSSGGSAAALAAGFVPIATT
                     SDGGGSVRIPAALCGLVGLKPTTGSVGRNVLPRWIELSTQGVTASTVADVVLQAQLVA
                     GASPGDWLSAPTAPGQFTPTRPASVRACRTFRADVDPVVEAAYEASLDAIAADGIPVE
                     RVDSPCGAQAIIDWFFISSCELAHSLAGERDRWESFDTSLQDILQFGIGVSMEDYLAA
                     TRRRHELGARIDDTLGGDSVLALPTLNVQDWGPEGPAATDAGSVTGDPSIAANTPDIN
                     LTGHPAASVPMGLGDRGVPMGLQLVAPRWSDGLALGVAEVLERSRPWPLTAPGFTPFG
                     LG"
     gene            5292..5879
                     /locus_tag="PLZ72_10530"
     CDS             5292..5879
                     /locus_tag="PLZ72_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36774.1"
                     /translation="MPNNDGGGMSGAPRDEVSRSVLREAIVLAHTVPGAVLRLVTHRG
                     EVTVGRGPDHDLSPCDLRQAYLVGTAPGRRDCFPVVREVELGGVVRELGGGLYEAWAP
                     DLTSVQRWFVTSLFPGDIVELADGWDEDGDLPVESVRADVATDTALGLSSVRLDVGDD
                     DPEGHLDEVSRRFLERCMAEELVQSVASEQIPRFS"
     gene            complement(5992..7605)
                     /locus_tag="PLZ72_10535"
     CDS             complement(5992..7605)
                     /locus_tag="PLZ72_10535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNA36775.1"
                     /translation="MPTHPRSTERSDAVVLAVDLGTGGPKTAYVDLAGNVIDHEHRRV
                     PIHTAPDGTATEDATHWWDAVVASARALSERGAVDPARIVAVACTGQWGSTVPVGGDG
                     RPVGDCILWLDQRAHRLAAEQLGGRTAVEGYRPKVALDFIRITGGAPSPLGNDPMGQR
                     LWIRHHQPDVYAETVHFMEPVDYLNLRFTGKVAATQASMLASFLTDNRVLDTVAYDER
                     LCRYAGTDVDRLPPLQPIRSVVGDVLPEVADELGIPAGIPVVTGLPDLHAATLGSGAV
                     DNFHGHLSISTSAWVGAHTPSKKTSLMSQMATVPSALAGQYVLVNNHDCGGASMEWLL
                     DMVVAADDGLTPGASPTLRDLDRVAEKVAAGADGVMFAPWLKGMRSPSADTAVRAAFL
                     NIGIDSGRPQMVRAVLEGVAHQVRWLLEVSEDVVKHQLVDLRAIGGGAQSDVWCQIHA
                     DVIGRPVHRVADPLVANVRGAALFAGLVTGRLEPGDVAAGATVDRVFRPDPEASAHYA
                     ERHREFVGLHKELKGVARRLHRLASRPTP"
     gene            complement(7703..8857)
                     /locus_tag="PLZ72_10540"
     CDS             complement(7703..8857)
                     /locus_tag="PLZ72_10540"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNA36776.1"
                     /translation="MRTGPGDEPESTDNDSDTHTAGPLRVVDPAERTPGPGPSGLTAE
                     EMADFGAEMDRLRQEILDSLGEADAAYIRRVIDTQRRLEAAGRLALFAGILPPAWVAG
                     TVMLATAKILENMEIGHNVMHGQWDWMRDPEIHSTTWEWDNTCPSSQWKHTHNQTHHV
                     WTNVLYKDRDVGYGLLRITRDQRWHPVYLAQPIYAAVLGMLFQWGVALHDVEVDQLLN
                     GRADAAVSRERLKEVGRKAGRQVLKDYVAFPLLAGPFFLPVLAGNMAANLIRNVWSFT
                     VIFCGHFPGRVPTFTAADVVGEDRDGWYVRQVLGSANISGGPLMDLMTGNLSYQIEHH
                     LFPDLPSNRYAEIAPRVRESCERHGLPYVTGSLPRQFGSVIARLFKMAIP"
     gene            complement(8902..>9753)
                     /locus_tag="PLZ72_10545"
     CDS             complement(8902..>9753)
                     /locus_tag="PLZ72_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HNA36777.1"
                     /translation="LLTITVQQVPDGIVSNHLVQRIRPGDVVHLDGPEGDFTLDPGPA
                     PTTGADHPPTAPGRPLLFITGGSGITPVIGMLRALDSGADTAPDATLDDVVVVHHAPT
                     DRESLFTDELDAMARRNRGLRVVHVRTGAGAPPEMELNAGRLDDLCPDWRDREAWTCG
                     PRPMVEAVESLWSGPDHATTRALHVERFTPSIPDGLPEVEGTLTFRAAGVSVPSDGTR
                     TVLDAAEDAGLSPLAGCRMGVCRRCVVPLHSGTVVDRRDGRIECEPGAHVQICVTAAV
                     GDVEIEI"
     assembly_gap    9754..9853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10041..>11095)
                     /locus_tag="PLZ72_10550"
     CDS             complement(10041..>11095)
                     /locus_tag="PLZ72_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA36778.1"
                     /translation="TLGLGFLFFVAGYEIDTARIRGTPFNRAATSWLISVALGLVVAG
                     VMVASGFVLSELLIGLALTTTAIGTLLPMMQDRNLLGTRFGDLLASAGAVGEFGPVVA
                     VTIFLGASSPTTEALLLIVFVLAALGLAAFMRRPPSPRVVEVMRRHMDTSSQLPVRVV
                     MLLIAAMIVVAVGLGLDMLLGAFAAGMLGRLLFPPSQSHALAAKLNSIGYGFLIPVFF
                     VVSGMGLDVEALTSSWTVAARVPLFLGLFLIVRGAPALLVYRGLPHRSRAALAILQCT
                     ALPLLVVITEIGLETGHMKPENAAALVGAGMLSVLVLPLWGFAVLGDGAERSGSSSVV
                     DGRGERPTPSGLPLTE"
BASE COUNT         1562 a         3716 c         4157 g         1560 t
ORIGIN      
        1 accgacaggt cgccggccga gcgggccagc tcgacccgac cccggacgac cagggcggtg
       61 gccgacgagt cggtcgggtc gaccttgtag acacccgcgg agtggccggc caccgagtag
      121 cggtcgaacg atacgttgcc gccgacggcg atgggaccgg tggagccgtc cgcggtcagg
      181 gcggcgtcgc cgtgggcgac ggcgctgaag cccgcggcga gggtcgcggg gttgaccgat
      241 gcagcggcgc agaactgcac ggtgctgacc gccgggatcg acccgctggc cggcgggatg
      301 ccggtgttgt cgaaggtgcc ggtcgtcgac gccggggtga tccaggcgtc cttggagccg
      361 tcgccggcct tcacgaccac ggcactgatc gcggcggccg acgcgaacgt gccgccggtg
      421 gccgaagtgg ccgacagcga cacgaccttg gcgttcccgg ccggctccac gaacgagtag
      481 gtgccggtgg agggcaggcg gctgaacttg gcgaccagtc gggtcccgct cggacacacc
      541 ggctcggggg cggcgccggt gctggcgaag atccccagac ccatcaggac ggcaccgacc
      601 acggccaggg cgatcccgcc ccgggtcggg gtgatgcgcg gtgcgctcgg agcagtcggc
      661 gagctctcgg tgctggacat gctgggacgt gcctccgtga gggcggtcgc gggcgcgcga
      721 ccttcgatcc cttcggtcgg tcgcttcgtc gcggacttga ggactcgtcc gccacatgcc
      781 tcccatcgcc gtggtccggt tcgtgtcggg tccgacgccg gggtggggtc cgcgttgagg
      841 gttccaggca gccgccctac gctgcgccgt caccggttgc ggccgggcgg gcgcgtccac
      901 gtccgccggg tgtgtcgacc gagcccacgt agctcagggg atagagcgtc ggtttcctaa
      961 accgtgcgtc gcaggttcga atcctgccgt gggcgcccag tggatcgtga ggtcccgggc
     1021 gcgggtgagc gctgccgaac agcgtcggtc gaccccgacc ttcgcgtgcg agatcgacca
     1081 ccgggaggtc gatcccacac gcggacatcg ggcgctggag ctccagctgc tcgactcggt
     1141 cgtcgacgga tcgggcaacc gctacgtcca ctccgagggc ggggactcgc cgtaccgcgg
     1201 gctgatcgcc ctcggctgag cggtcagtcc ttgcggtaga cggtgaccac ggcagcgccg
     1261 cccagaccca ggttgtgctg gatgccgacc cgggccccgt cgacctggcg agcctcggcg
     1321 tcgcctcgga gctgccaggt gatctcgctg cactgggcga ggccggtcgc tcccaacggg
     1381 tggcccttgg agatgagccc gccgctcggg ttcaccaccc accggccgcc gtaggtgacc
     1441 gcaccgtcgt ggatgagctc gcccgccttg ccctcggcgc acagcccgag gccctggtag
     1501 gtgatcagct cgttggcgct gaagcagtcg tgcagctcga tgacctgggc gtcctcgggc
     1561 cccaggcccg actcctcgta ggcctggcgg gcggcggcct cggtgaggtg gatgccggcg
     1621 atcgccatgt cgcttccctc gaaggtgtcg tcgaagtcgg tggtcatcgc ctggcccgcg
     1681 atctccaccg cccggtcctc gaggccgtgc tccttgacga agcgctcgct ggccaccacc
     1741 gcggcggccc cgccgtcgga ggtggggcag cactgcagct tggtcaacgg gtcgtacacc
     1801 gtcggcgcgt cgaggatgtc ctggaggctg tactggtcgc ggaactgcga gtacgggttg
     1861 ttgaccgagt ggcggtggtt cttctcgccg atgcgggcga aggtctcggc ggtgacgccg
     1921 tagcgctcca tgtactcccg gccggcgttg ccgaacagct gggcagcggg cggggcgggc
     1981 tcgaacccgc ggcgctgcac catgtcggtg aagtgcttgt ccatggggtt ggtgcggtcc
     2041 tcgaagttgc ccttgaggga gcccttctgc atcttctcga aaccgagggc cagcacgcag
     2101 tcggccaggc cgccctggac gaagcggcgg cccaggtaca gcgccgagct gccggtcgag
     2161 cagttgttgt tgacgttgac ggcggggatc ccggtgaggc cgagctcgta cagcgcccgc
     2221 tggcccgagg tcgagtcgcc gtagcagtag ccgatgaacg cggactcgat gtctgagtag
     2281 tcgatgccgg cgtcggccag cgccttctcg ccggcttcgc ggcccatgtc ggggtagtcc
     2341 cagtcgcggc tgccgggctt ctcgaagcgg gtcatgccga cgccgatcac ataggtcttc
     2401 gtggtcatgg tcctcacggt aggcgtcggg ccgctggcgg ggtggccccg aacagctgga
     2461 gcagcgcggc ggccggatcg gtcgccggtc gagcgcgggt tccggtggcg gcgagtcccg
     2521 cgtcgtcacc gctgcggtac tgcgacatgg tcggctccac gtacgggttc atgacctggt
     2581 gctgaccctc gaacgtctcg ctgtggtggt ccaggccgac ggcgtgcccg agctcgtgca
     2641 gcagcaccgc ccgtcgggtc gccgcgtcgt cgagcatggc gggcaccacc gagagccgga
     2701 cgtcgcggat cacgtggcgt tcttcgggcg tgaagcccca ctggccccac atctcggcgc
     2761 agccgatcgc accggccgag cagtacgtgc cgacgacgac ggtgatctcg ggggtcgagc
     2821 cgaccaggtc gggggccggg gtcggcgccg gcgggccgag gacgaaggtg atcccggagc
     2881 gggcggtgac ctcggccgcg gcggccatga cctcgtcggt cagcgacccg gccgagggat
     2941 ggacccgaac gacgaagctc gatgacggcg cccactcggt cagccggtag cccggccctg
     3001 acggcggctg cggttgcggg gcccgggggg ccgcgctgca cgcggcgagg atggcaagga
     3061 cggcgacggc tgccgccgcg gtggcggatc ttgcgacctg tcgtgggctc ggacgcatcg
     3121 gtgatcctcc gggcgactcg gcgactggac ctgatgcagc ctcttcggat gcggtgcgga
     3181 acgggttgag cacgatgatc cggactgctc agcccgggaa caaccgcgtg ggccctgcgg
     3241 ttggctccgg tggtccgacc gacctcgcga cctgggccga tcggtcccgg acgacggccg
     3301 gtcccgccgg cggtagccga cgagcgtcgt cgggccgccc acaccaggag atgacatggc
     3361 tctcgatccc gacctcaagg acctcgcgca ggggaagaac ttcgcggcgc tcaccactct
     3421 catgcccgac ggccaccccc agacccagat catgtgggtg ggcgccgatg acgagcacgt
     3481 gatcatcaac acgcagctcg accgccagaa gtaccgcaac gtcctcgcag atccccgcgt
     3541 caccgtgacc gtgttcgatg ccgacaaccc gtaccgctac gtcgaggccc gtggtcgggt
     3601 cgcgcgcacc caggacggcg cggccgccgc ggccagcatc gacgagctgt cgcgcaagta
     3661 caccggtggg gactacggca tggggccgac ggacagtcgc gtcatcctgt tcatcgacgt
     3721 ggagcgggtc cacaagaacg gatactgacg gcgggccccg acgggtcggg tccgttcagt
     3781 cggatccgat cagtcggatc cgttcagccg agcccgaagg gggtgaagcc cggcgcggtc
     3841 agcggccagg gacgcgagcg ctccagcacc tcggccacgc cgagcgccaa cccgtcggac
     3901 cagcggggcg ccaccagctg cagacccatc ggcacgcccc ggtcgccgag tcccatcggc
     3961 acgctcgcgg cggggtgccc ggtcaggttg atgtcggggg tgttggctgc gatcgacgga
     4021 tctccggtga ccgagccggc gtcggtagcg gccgggccct cgggccccca gtcctggacg
     4081 ttcagggtcg gcaacgccag gaccgagtcg cccccgagcg tgtcgtcgat ccgggcgccg
     4141 agctcgtggc gccgtcgggt ggcggccagg tagtcctcca tcgacacgcc gatgccgaac
     4201 tgcaggatgt cctgaagtga ggtgtcgaag ctctcccagc ggtcgcgttc cccggccagg
     4261 ctgtgggcga gctcgcagga cgagatgaag aaccagtcga tgatcgcctg ggcgccgcag
     4321 ggggagtcca cccgctcgac ggggatgccg tcggccgcga tggcgtcgag ggatgcctcg
     4381 taggcggcct cgacgacggg gtcgacatcg gcccggaagg tccgacaggc ccggacgctc
     4441 gccggtcggg tcggggtgaa ctggccgggg gccgtcggtg ccgacagcca gtcgcccggc
     4501 gacgcgccgg cgaccagctg cgcctggagg acgacgtcgg cgacggtgct cgcggtgacg
     4561 ccctgggtgg acagctcgat ccagcggggc agcacgttgc gtcccaccga gccggtggtc
     4621 ggcttgaggc cgaccagccc gcacagcgcg gcgggtatcc ggacgctgcc gccgccgtcg
     4681 gaggtggtcg cgatcgggac gaacccggcg gccagcgccg cggcggaccc tccgctcgag
     4741 ccgccggggg agcagtcggt gttccacggg ttgcgagtgg cgccgaacac gaggttggcg
     4801 gtgaacggcg cgtggccgaa cgccggggtg ttggtccggc cgaggacgat cgctcccgcg
     4861 gcccgcagcc gggcgaccgc gtcgtcgtcg ctgtcgtcgg ggtcggcgtc gacgaacatc
     4921 gcggacccgt aggtggtgac cgagccggcg cagcgggcca tgtccttgac caggaacggg
     4981 aggccggcca gcggaccgga tcggtcgtgg cccgccgcga cctcgcgggc ggcgtcggcg
     5041 tcgagtcgga ccacggcgtt gatcgcgccg tcgccggcgt cgatgcgacg gaccgcctcc
     5101 tcgaccaggt cgacggggtc gatcgccccg gattgcacac cgtggaccag ttcgctcagc
     5161 acagcggcga cgctaccggc gcccccaccc ctcacccgtc gcgttctgac gacaagtcac
     5221 gacactggcg gtactcgact gtcatgagaa tcgcgttgac gagtctgcgg accgactcta
     5281 ggttattagg tatgcctaac aatgacggtg gcgggatgag cggcgctccc cgggatgagg
     5341 tgagccggtc cgtgcttcgc gaggcgatcg tgctcgccca caccgtgccg ggagcggtgc
     5401 tgcggctggt cacccatcgg ggcgaggtga ccgtcgggcg cggtcccgac cacgacctgt
     5461 caccgtgcga cctccggcag gcgtacctgg tcggcactgc cccgggtcga cgtgactgct
     5521 tccccgtcgt ccgggaggtc gagctcggcg gcgtcgtgcg cgagctcggc ggcggtctct
     5581 acgaggcgtg ggctccggac ctcacctcgg tgcagcgctg gttcgtcacc tcgctgttcc
     5641 ccggcgacat cgtcgagctg gccgacgggt gggacgagga cggtgatctc cccgtggagt
     5701 cggtgcgggc cgacgtcgcc accgacaccg cactcggcct gtcgtcggtg cgcttggatg
     5761 tcggcgacga cgacccggag ggtcacctgg acgaggtgtc gcgtcggttc ctcgagcgct
     5821 gcatggccga ggagctcgtc cagtcagtcg cctcggagca gatcccccga ttctcctgac
     5881 ggcccgatct ccagacaggc ccgactttct gcggacggct ccgattctcg tgacagttga
     5941 gtaccgccag tgtcgtgact tgtcgtcaga acctggggtg agaagcgggg gtcagggggt
     6001 ggggcgggag gcgaggcggt ggaggcggcg cgccacgccc ttgagctcct tgtgcaaccc
     6061 gacgaactcg cggtgacgct cggcgtagtg cgccgaggcc tccgggtcgg gccggaacac
     6121 ccggtcgacg gtcgcgcccg cggccacgtc gcccggctcc agccgcccgg tgaccagacc
     6181 ggcgaacagc gccgcgcccc gcacgttggc caccagggga tcagccaccc ggtgcacggg
     6241 tcggccgatc acgtccgcgt ggatctggca ccacacgtcg gactgggcgc cgccgccgat
     6301 cgcccgcagg tcgaccagct ggtgcttgac cacgtcctcg gacacctcga gcagccagcg
     6361 cacctggtgg gcgacgccct cgagcacggc gcgcaccatc tgtgggcgtc ccgagtcgat
     6421 gccgatgttg aggaacgcgg cgcgcaccgc ggtgtcggcg ctgggcgagc gcatgccctt
     6481 gagccacggg gcgaacatga cgccgtcggc gccggcggcg accttctcgg ccacccggtc
     6541 gaggtcccgt agcgtcggcg acgccccggg ggtgaggccg tcgtccgcgg cgaccaccat
     6601 gtcgaggagc cactccatcg acgccccgcc gcagtcgtgg ttgttgacca gcacgtactg
     6661 gccggccaac gccgagggca ccgtagccat ctggctcatc agcgacgtct tcttggacgg
     6721 cgtgtgggcc ccgacccacg ccgaggtgct gatcgacagg tggccgtgga agttgtcgac
     6781 cgcaccggag ccgagagtgg ccgcgtgcag gtcgggcagg ccggtcacca ccggtatgcc
     6841 ggcggggatg ccgagctcgt cggccacctc gggcaggacg tcaccgacca ccgagcggat
     6901 cggctgcaac ggcgggaggc ggtcgacgtc ggtgccggcg tagcggcaga ggcgctcgtc
     6961 gtaggcgacg gtgtcgagga cccggttgtc ggtcaggaag ctggccagca tcgacgcctg
     7021 ggtcgcggcg accttcccgg tgaagcgcag gttgaggtag tcgaccggct ccatgaaatg
     7081 gacggtctcg gcgtagacgt cgggctggtg gtggcggatc cacaggcgct ggcccatcgg
     7141 gtcgttgccg agcgggctgg gggcgccgcc ggtgatgcgg atgaagtcga gcgccacctt
     7201 gggtcggtag ccctcgaccg cggtccgccc gccgagctgt tcggcggcca ggcggtgggc
     7261 ccgctggtcg agccacagga tgcagtctcc gaccggtcga ccgtcgccgc cgaccgggac
     7321 ggtcgatccc cactggccgg tgcacgcgac cgccacgatg cgggcggggt cgacggcgcc
     7381 gcgctcggac aacgcccggg ccgaggcgac caccgcgtcc caccagtgcg tggcgtcctc
     7441 ggtggcggtc ccgtcggggg cggtgtggat cggcacccgt cggtgctcgt ggtcgatcac
     7501 gttgccggcc aggtcgacgt acgcggtctt ggggccgccg gtgcccaggt cgaccgccag
     7561 cacgaccgcg tcggagcgct cggtcgagcg ggggtgggtc ggcatgggcc gacacgctac
     7621 cggacgggtt ctgcggtcct cgtccccgtc ggggtcccgg cgggctgccg ttggcgacac
     7681 cccgggtcgg gggtggcggc ggtcagggga tggccatctt gaacaggcgg gcgatcaccg
     7741 agccgaactg gcggggcagc gaaccggtca cgtacggcag accgtgacgc tcgcaggact
     7801 cgcgcacccg aggggcgatc tcggcgtagc ggttcgacgg caggtccggg aacaggtggt
     7861 gttcgatctg gtagctgagg ttgccggtca tcaggtccat cagcggaccg ccggagatgt
     7921 tggccgaacc gagcacctgg cggacgtacc agccgtcgcg gtcctccccg acgacgtcgg
     7981 cggcggtgaa cgtcggcacc cgaccgggga agtgaccgca gaagatgacg gtgaacgacc
     8041 acacgttgcg gatcaggttg gcggccatgt tgccggccag gaccgggagg aagaacgggc
     8101 cggcgagcag cgggaacgcc acgtagtcct tcagcacctg gcggccggcc tttcggccga
     8161 cctccttgag acgctcgcgg gacaccgcgg catcggcgcg gccgttgagg agctggtcga
     8221 cctcgacgtc gtgcagggcg acgccccact ggaacagcat cccgagcacc gcggcgtaga
     8281 tcggctgggc caggtacacc gggtgccatc gctggtcgcg ggtgatgcgc agcaggccgt
     8341 agccgacgtc gcggtccttg tagaggacgt tggtccacac gtgatgggtc tggttgtggg
     8401 tgtgcttcca ctgcgaggac gggcacgtgt tgtcccactc ccaggtggtg gagtggatct
     8461 cggggtcgcg catccagtcc cactggccgt gcatgacgtt gtggccgatc tccatgttct
     8521 cgaggatctt cgccgtggcg agcatgacgg tcccggccac ccacgccggg gggaggatcc
     8581 cggcgaacag ggcgaggcga ccggcggcct cgagccggcg ctgggtgtcg atcacccggc
     8641 ggatgtaggc ggcgtcggcc tcgccgagcg agtcgaggat ctcctggcgg agacggtcca
     8701 tctcggcgcc gaagtcggcc atctcctcgg cggtcagacc cgacgggccg ggcccgggcg
     8761 tgcgctccgc cgggtcgacg acccgcaggg ggccggcggt gtgggtgtcg gagtcgttgt
     8821 cggtggactc cggctcgtcg ccgggaccgg ttcgcatgac ggcgaccatg gttcctcctg
     8881 gggggtgccc gccggagcgg gtcagatctc gatctccacg tcgccgaccg cggcggtgac
     8941 gcagatctgg acgtgggcgc cgggttcgca ctcgatgcga ccgtcgcgac ggtcgacgac
     9001 ggttccggag tggagcggga cgacgcagcg tcgacacacc cccatccggc atccggcgag
     9061 gggtgacagg ccggcgtcct cggcggcgtc gaggacggtc cgggtgccgt cgctcggcac
     9121 cgacaccccg gctgcccgga acgtgagcgt gccctcgacc tcgggcaggc cgtcggggat
     9181 cgacggggtg aaccgctcga cgtgcagcgc ccgcgtcgtc gcgtggtcgg gcccgctcca
     9241 cagcgactcg acggcctcga ccatcgggcg gggcccgcac gtccaggcct cgcggtcgcg
     9301 ccagtcgggg cacagatcgt cgaggcgccc ggcgttcagc tccatctcgg ggggcgcgcc
     9361 ggcaccggtg cggacgtgca cgacccgaag tccacggttg cgtcgggcca tggcatcgag
     9421 ctcgtcggtg aacagcgact ctctgtcggt cggagcgtgg tgcaccacga cgacgtcgtc
     9481 gagggtggcg tccggtgcgg tgtccgcacc tgagtcgagg gctcgcagca tgccgatcac
     9541 cggggtgatc ccgctgccgc cggtgatgaa cagcagcggt cgacccggag cggtcggtgg
     9601 gtgatcggcg ccggtggtcg gtgcggggcc cgggtccagg gtgaagtcgc cctcgggccc
     9661 gtcgaggtgc acgacgtcgc cgggtcggat gcgctgcacc aggtggttcg acacgatgcc
     9721 gtcggggacc tgctgcacgg tgatcgtgag cagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnncgtctcg ggcgtgaccg acacgacctc ggctcggacg tcgacgcccg
     9901 gggcgagagg gtcgaccagg cgccggtagg cgccggacgg ggcgggccag ctcagcacgg
     9961 cggcgagccg ggcgagcggg cggaaggggc cggtcggcac ggcggtgggg gccacgggcc
    10021 cataacttac cggcggtaac ctattcggtc aacggcagcc cggacggcgt cgggcgctcg
    10081 ccccgaccgt cgacgacgct cgacgaaccg gagcgctcgg cgccgtcacc cagcacggcg
    10141 aagccccaca gcggcagcac gagcaccgac agcatcccgg ctccgacgag cgcggcggcg
    10201 ttctcgggct tcatgtgccc cgtctccagg ccgatctcgg tgatcaccac cagcagcggc
    10261 agcgccgtgc actggaggat ggccagagcg gcgcggctcc gatgaggaag accccggtac
    10321 acgagcagcg cgggggcgcc ccggacgatc aggaacaggc cgaggaacag cgggacgcgt
    10381 gcggccacgg tccagctgga ggtgagggcc tcgacgtcga gccccatccc gctcacgacg
    10441 aagaacaccg ggatcaggaa cccgtagccg atcgagttga gcttggcggc cagggcgtgg
    10501 gactgcgacg gggggaacag cagccgtccg agcatccccg ccgcgaacgc acccaggagc
    10561 atgtccagac cgagcccgac ggcgacgacg atcatcgctg cgatgagcag catcaccacc
    10621 ctcaccggca gctgcgacga cgtgtccatg tggcggcgca tcacctcgac cacacgtggt
    10681 gacggcggcc ggcgcatgaa cgctgccagt ccgagcgcgg ccagcacgaa cacgatcagc
    10741 agcagcgcct cggtggtcgg gctggacgca cccaggaaga tcgtgaccgc caccaccggg
    10801 ccgaactcgc cgaccgcgcc ggccgaggcg agcaggtcgc cgaagcgggt gccgaggagg
    10861 ttccggtcct gcatcatcgg caggagcgtg ccgatggccg tcgtggtcag cgccagaccg
    10921 atcagcagct ccgacaggac gaagccggac gcgaccatca ctcccgccac gaccaggccg
    10981 agggcgaccg agatgagcca ggacgtcgcg gcgcggttga acggcgttcc ccggatgcgg
    11041 gcggtgtcga tctcgtagcc ggcgacgaag aacaggaacc cgaggccgag cgtgg
//
LOCUS       DAOQOD010000103        11058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2104_length_11058_cov_12.020267, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000103 DAOQOD010000000
VERSION     DAOQOD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_11058_cov_12.020267"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..842)
                     /locus_tag="PLZ72_10555"
     CDS             complement(75..842)
                     /locus_tag="PLZ72_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNA36779.1"
                     /translation="MPRFAVLDGPTPIAFAHRGAHEQVPENSLRAFEEAHRLGYRFFE
                     TDVHVSADGRLYAFHDDVLDRVTDRTGPLSALPAAELARVRIDGTDPIPTFDELLESF
                     PDVRWNVDPKSDGAVMALTRALRRHDAVRRVNVGAFSDDRLGRLRSLLGPELSTAAGP
                     REVAALTVSARVPAGRRRRSTAPYSCLQIPVRYRNVELVTGALLDAAHARGAQVHVWT
                     IDDAAEMHRLLDLGVDGIITDRPQVLRSVLEARGAWS"
     gene            complement(964..2538)
                     /locus_tag="PLZ72_10560"
     CDS             complement(964..2538)
                     /locus_tag="PLZ72_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA36780.1"
                     /translation="MSDELFYPGAHAESRPDAIAYRMVGADGTPGAEVTYAELHERAN
                     RIAHLFRSLGLHPGDHVAFCLENHPWFLSLAWGAHYAGLYYTAISSRLTAEEIAYIVD
                     DCGARVFITSGYKADVAEAVAAATSGVEHRFMLDGTVGGYEPFLDAVAAQPSTDLPDA
                     VEGMDMLYSSGTTGRPKGVKVPLRGVPLGTPDPLFMLVAGLFGANPDTIYLSPAPLYH
                     AAPLRFCLQVQRAGGTVVVMEHFDPEQTLANIERYRVTFGQFVPTMFVRMLKLDPAVR
                     DRYDVSSVQVAVHAAAPCPVAVKHQMIEWWGPVLHEYYAGTEGNGFVYTGSEDWLAHP
                     GTVGKALVGTLHIVGEDGEELPVGESGTVYFEGEANFSYHNDPAKTAESYLKEGWSTL
                     GDVGYLDEDGFLYLTDRKAYMIITGGVNVYPQEAENLLTMHPKVSDVAVFGVPNDDFG
                     EEVKAVVAPVRMPADDDEAAALSTELIAYCREHLSGVKCPRSVDFREELPRHPTGKLY
                     KRLLKDEYWAAAGRTI"
     gene            2674..3459
                     /locus_tag="PLZ72_10565"
     CDS             2674..3459
                     /locus_tag="PLZ72_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNA36781.1"
                     /translation="MKDADAVAEFGALVAGGAEPLDRALALIATAGRPDVDPDAVIGR
                     IDELADMVPAGDSVQMCRGLFTGLGFHGNRTDYYDPDNSRIDRVIDRRTGIPITLSVL
                     AMEIGRRRGVTLLGIGMPGHFLLRDASDDDLFLDAFDGGRVLDLAGCRRLFDGLHGPT
                     APFDPAFLTPTGPVDIVTRVLNNLRAAHLRGGDRHGFVDVLRLQAAMPGAGVGERRQL
                     AGVLAEEGRFLDAALVHDRLADEDHARADEHRAASVRLRARLN"
     gene            3596..4816
                     /locus_tag="PLZ72_10570"
     CDS             3596..4816
                     /locus_tag="PLZ72_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36782.1"
                     /translation="MSLTEPSTGAEVDAKVDADEQRVIDAVERLLAEHPPADTPEKEF
                     LEAQYDAGLAWVHFPEGLGGLGVSPKLQRTVIQRVSAAKGPMGGAKNPIGYGMCAPAV
                     LTHGTDEQKQLLRPLFSNEHIWCQMFSEPGAGSDVASLAMKAERDGDEWIVNGQKVWT
                     TLAHIARYGLVICRTDPDLPKHRGITAFIVDMELPGVEVRPLRQMTGEAEFNEVYLTD
                     VRIPDSMRVDEVGRGWGVSITTLMNERVSIGGGTPPRGSGPIGQLLEEWKRSGSDDPV
                     TRDAVTQLWIQAEVNRLTNLRAAANRKAGTPGPEGSVAKLAMAELNKKIFSLAVDIMG
                     PEGMLYGSYEFVRPRHALFTDDMHKNFLRARANSIEGGTSEVLRNILGERVLGLPGDV
                     RVDKDLPWAQVPRS"
     gene            4915..6348
                     /gene="pyk"
                     /locus_tag="PLZ72_10575"
     CDS             4915..6348
                     /gene="pyk"
                     /locus_tag="PLZ72_10575"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNA36783.1"
                     /translation="MPRRTKIVATIGPASDSPDVLRRLIEVGADVIRIGLAHGTLDDA
                     MARYRTVRAVSEELGRHIGVLIDLPGPKVRLGSFGDSPTILDTGAPLTLIPGRDTSDT
                     SVLGVDYHNLLKDIHPGDHLPVGDGSVVIQVESVDGDAAKATVLYGGPVQGRPGLHIS
                     SDRLSLSTPTPTDLHLLDAFVDEGVDMVALSFVRSAHDIRRIGTEPHPRGPLVVAKIE
                     TRAAVENLQGIIEASGAIMIARGDLGTELGIEELPHLQKQITRDCISGGKPVITATQM
                     FESMITSPTPTRAEVSDVANAIFDGTSAVMLSAESAVGIDPVNAVATMSRVAERADQE
                     FDYDAWARRIRHLRRSAVGNSSEDKLTDAMTAAAWRAATEMGVAAIICISDSGLTVRS
                     TARFRPAMPLIGFSPDDRTLRQLSLSWGTTTLRAPRHVDTIALMDDLIVQARDAGLVR
                     PGQTVAVLAGAGSRSRSTDVLHLARVP"
     gene            6354..7217
                     /locus_tag="PLZ72_10580"
     CDS             6354..7217
                     /locus_tag="PLZ72_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36784.1"
                     /translation="MSSPDPAPPAPLSHDVVDGLGVIRRRPPGGDPSRPAVVLVHGAM
                     DRAASFGRVMRRLGDLDVCAYDRRGYATSLSAGTADDLDAQAADLHRVARWTGADRVV
                     VVGHSLGGTVALRWAEGAPDGLVSLGAFESPVPTLDNYRSGGADVALAADRTGGPRAA
                     AEAFYRFVVGDRTWDGLRERDRETRRAEGAQLVAELRDLQRPDGSPRLDAVAVPVVVG
                     LGGASVGAWRAAAEELAERLPRATRFDIAGAGHGAHLSHPDEFARYVRAVLDAADDAG
                     REPVASAPWPP"
     gene            7205..8293
                     /locus_tag="PLZ72_10585"
     CDS             7205..8293
                     /locus_tag="PLZ72_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNA36785.1"
                     /translation="MATLKSLTRRKPRVAFVLSGGGNLGSLQVGMLKALTEHGIVPDV
                     VLGCSVGAMNGAAYALDPTGAGMTRLEEHWRGAFEEVMPSSRVPSMLQLIRKGESLHA
                     ADGLRRGLEVLLGATRRFDELTVPFQCVAAEIETAAEHWFEDGYLVPAVLASAALPAV
                     FPAVTVEGRRYLDGGVVDNVPISRAVELGCREIYVLHVGRHGKPDAEIRRPIDAALQA
                     YWVARNGRFARDLAALPDGVEAVVLPPGQRPDIRYDDFSHTAELIDRGYENSMTLLAE
                     RQALDAERRIRSDVLQPLERWMHSARNRTWRRAAQDVAVDPGGGILTAPDRSAGFDET
                     DGGDLADDGGEVTSPVRPGSGAAGPGKT"
     gene            complement(8347..8787)
                     /locus_tag="PLZ72_10590"
     CDS             complement(8347..8787)
                     /locus_tag="PLZ72_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HNA36786.1"
                     /translation="MTDRPVNLNPSGPPETILDPEPAATESALEAALSRPTDEQRRAV
                     ADVVAANPRCLQAWAELGDRGRDDVESYAYYRIGYHRGLDRLRASGWRGSGYVRWAHP
                     TNRGFLRSLAGLRAMAAQIGEVDEEERCRHFLAQLDPDHFSPEP"
     gene            complement(8877..9023)
                     /locus_tag="PLZ72_10595"
     CDS             complement(8877..9023)
                     /locus_tag="PLZ72_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36787.1"
                     /translation="MRLLLDQNPSPLLAEQLVADDLDAGAIVVLDDDRIRVRRLPLRP
                     PEDR"
     gene            complement(9020..9229)
                     /locus_tag="PLZ72_10600"
     CDS             complement(9020..9229)
                     /locus_tag="PLZ72_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNA36788.1"
                     /translation="MDPKRMGGVPFVRDLRFPVASIVAMVADGMSTDEILAEHPDLEA
                     EDVVESLRYAALAVQERELPLLRPA"
     gene            complement(9382..9669)
                     /locus_tag="PLZ72_10605"
     CDS             complement(9382..9669)
                     /locus_tag="PLZ72_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNA36789.1"
                     /translation="MTTSTHSSSAGPGGPVSDQLVEQFADEAEQGYDVERLVARRGRR
                     GRPRLGSEPSTVESVRLDPELKERLVRRAEADGVAVSEVIRDALRHHLEAS"
     gene            9711..9947
                     /locus_tag="PLZ72_10610"
     CDS             9711..9947
                     /locus_tag="PLZ72_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36790.1"
                     /translation="MDDHLGTVGADENDDLQQIAGSVGPEHEPPVRVLPDVLDSQGVL
                     ERMEHLVVSDAVTSGCSVDLHTGLLYYVIAKVGV"
     gene            10156..10530
                     /locus_tag="PLZ72_10615"
     CDS             10156..10530
                     /locus_tag="PLZ72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNA36791.1"
                     /translation="MEGTDAIHGRQKQAGVGDDRRLLEPTWPRGRIDGFGCRCDQRRS
                     YTSVMAYERITVDPARMGGVPTIRDTRVTVGMVLGQLAAGRTVDDVLADYPYLERDDI
                     WGALEFAAAAVNEREVPVARPA"
     gene            10527..10895
                     /locus_tag="PLZ72_10620"
     CDS             10527..10895
                     /locus_tag="PLZ72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNA36792.1"
                     /translation="MRLLIDANLSPRVAEGLRSAGYDAGHVADLDMVTATDEQIFDRA
                     VADDLVVVTADSDFGKLLALRRASNPSVIHVRGVAELAPEVHLDLLVRNLPTIAGDLR
                     RGVIASLSPARLALRDLPLR"
BASE COUNT         1642 a         4021 c         3824 g         1571 t
ORIGIN      
        1 gtaccgccgg cggagccggc gcgagcgcga ccagcaacgc caccaccagg ctcgccaccg
       61 cgctcatgcc gccctcacga ccaggcgccc cgggcctcga gcaccgagcg gaggacctgc
      121 ggtcgatcgg tgatgatccc atcgacgccg aggtcgagca gtcggtgcat ctcggccgcg
      181 tcgtcgatcg tccacacgtg cacctgagcc ccgcgggcgt gggcggcgtc gaggagcgcc
      241 ccggtgacga gctcgacgtt gcggtaccgc accgggatct ggaggcagct gtagggagcc
      301 gtcgaccggc gccgccgacc ggctgggacc cgggccgaca cggtcagtgc cgccacctct
      361 ctggggcccg ccgcggtgga cagctcaggt cccagcaggg accgcagccg accgagccgg
      421 tcgtcggaga acgcgccgac gttgacccgt cgcaccgcat cgtgtcgacg cagagcgcgg
      481 gtgagcgcca tcaccgcacc gtcggacttg gggtcgacgt tccagcggac gtcagggaag
      541 gactccagca gctcgtcgaa ggtcgggatc ggatcggtgc cgtcgatgcg gacccgggcc
      601 agctccgcgg cgggaagcgc cgacagcggt ccggtccggt ccgtgacccg gtcgagcacg
      661 tcgtcgtgga acgcgtagag gcgtccgtcg gcgctgacgt gcacgtcggt ctcgaagaag
      721 cggtagccca ggcggtgtgc ctcctcgaac gcccgcaggg agttctccgg cacctgttcg
      781 tgcgcgccgc gatgggcgaa cgcgatcggg gtcgggccgt cgaggacggc gaaccggggc
      841 atcacgcgag gctaccgggc ggcgtcgccg ccccgacccg cggccacccc cgcccgcgac
      901 cacccccacc gggcgcgaag tcggcgggat cggctccagg tcaggacctg agggccctcg
      961 aactcagatg gtgcggccag ccgcggccca gtactcgtcc ttgagcagcc gcttgtagag
     1021 cttcccggtc gggtgccggg ggagctcctc acggaagtcc accgagcggg ggcacttcac
     1081 ccccgagagg tgttcacggc agtaggcgat cagctcggtc gacagcgcgg ccgcctcgtc
     1141 gtcgtctgcg ggcatccgca ccggcgcgac caccgccttg acctcttcgc cgaagtcgtc
     1201 gttcgggacg ccgaacacgg cgacgtcgct gaccttgggg tgcatcgtca gcaggttctc
     1261 ggcctcctgt gggtacacgt tcaccccgcc ggtgatgatc atgtacgcct tgcggtcggt
     1321 gaggtagagg aagccgtcct cgtccaggta gccgacgtcg ccgagggtgg accacccctc
     1381 cttcaggtac gactcagcgg tcttggcggg gtcgttgtgg tagctgaagt tcgcttcgcc
     1441 ctcgaagtag acggtgccgg actcgccgac ggggagctcc tcgccgtcct cgccgacgat
     1501 gtgcagggtc ccgaccaggg ccttgccgac cgtgccgggg tgggcgagcc agtcctccga
     1561 cccggtgtac acgaagccgt tgccttcggt gccggcgtag tactcgtgca gcaccggccc
     1621 ccaccactcg atcatctggt gcttgaccgc caccgggcac ggcgccgcgg cgtgcacggc
     1681 gacctggacg ctcgagacgt cgtagcggtc ccggaccgcg gggtcgagct tgagcatgcg
     1741 cacgaacatg gtggggacga actggccgaa ggtgacccgg taccgctcga tgttggccag
     1801 cgtctgctcg gggtcgaagt gctccatgac cacgacggtc cctccggcgc gctggacctg
     1861 caggcagaag cgcagcggcg cagcgtggta cagcggggcg ggggagaggt agatggtgtc
     1921 ggggttggcg ccgaagaggc ccgcgaccag catgaagagc gggtcggggg ttcccagcgg
     1981 gacgccccgc agcgggacct tgacgccctt gggtcgaccg gtcgtccccg aggagtagag
     2041 catgtccatc ccctcgaccg cgtcgggcag gtcggtcgag ggctgggccg cgacggcgtc
     2101 gaggaacggc tcgtagcccc cgacggtccc gtcgagcatg aaccggtgct cgacgccgga
     2161 cgtggccgcg gcgaccgcct cggccacgtc ggccttgtac ccggaggtga tgaagacccg
     2221 ggcgccgcag tcgtcgacga tgtaggcgat ctcctcggcg gtcagccgag aggagatcgc
     2281 cgtgtagtag agcccggcgt agtgcgctcc ccacgccagc gacaggaacc acgggtggtt
     2341 ctccaggcag aacgcgacgt ggtcacccgg gtgcagcccg aggctgcgga acaggtgggc
     2401 gatccggttg gcccgttcgt gcagctcggc gtaggtgacc tcggcaccgg gcgttccgtc
     2461 ggcgccgacc atccggtagg cgatggcgtc gggacggctc tcagcgtgcg cccccgggta
     2521 gaacagctcg tcgctcatgg gcgggcaccg taccctccgt cgacggaccc cgtccccacc
     2581 ctgctctgtc ccgcacagac gcggtgatca ccgcgtctgt gcgggacaga gctgcgggac
     2641 agagtgggcg ccggggcgcg tacggtggca accatgaagg acgctgacgc ggtggccgag
     2701 ttcggtgccc tcgtcgccgg cggggccgaa ccgctcgacc gggccctcgc tctgatcgcc
     2761 accgccggcc gtccggacgt cgatcccgac gccgtcatcg gccgcatcga cgagctcgcc
     2821 gacatggttc ccgccggcga cagcgtgcag atgtgccgag gcctgttcac cggactcggc
     2881 ttccacggga accgcaccga ttactacgac cccgacaaca gccgcatcga ccgggtgatc
     2941 gaccgtcgga ccggcatccc gatcacgctg tcggtcctgg ccatggagat cgggcgacgg
     3001 cgcggggtca cgctcctggg catcggcatg ccggggcatt tcctgctgcg ggacgcttcc
     3061 gacgacgacc tcttcctgga tgcgttcgac ggtggccggg tcctggacct ggccggttgc
     3121 cgacggctgt tcgacggcct gcacggaccg accgcgccgt tcgacccggc gttcctcacc
     3181 ccgaccgggc cggtcgacat cgtcacccgg gtcctcaaca acctccgcgc cgcccacctc
     3241 cgaggcggtg accggcacgg cttcgtcgac gtgctgcgcc tacaggccgc gatgcccgga
     3301 gcgggcgtcg gcgagcgtcg ccaactcgcg ggggtgctcg ccgaggaggg tcgcttcctc
     3361 gacgcggccc tggtccacga ccgcctggcc gacgaggatc acgcccgggc cgacgagcac
     3421 cgtgccgcgt cggtgcgcct gcgagcccgt ctcaactgag tgcgcccgcg accgggcgcc
     3481 cacgcttctg ggggtgggaa tcgacacacc tcgtcgattg cgacccccag gttccggtcg
     3541 ggctaacttg accgatcggt caagttagcc cggcgacaca acagggggtc cagcgatgtc
     3601 gctcaccgaa ccgtccaccg gcgccgaggt cgacgccaag gtcgacgccg acgagcagcg
     3661 tgtgatcgac gccgtcgaac gcctcctcgc cgagcacccg ccggccgaca cccccgagaa
     3721 ggagttcctc gaggcccagt acgacgcggg cctggcctgg gtgcacttcc ccgagggcct
     3781 cggagggctg ggggtcagcc ccaagctgca gcgcaccgtg atccagcggg tcagcgccgc
     3841 caagggcccg atgggcggcg ccaagaaccc gatcggctac ggcatgtgcg cgccggcggt
     3901 gctcacccac ggcaccgacg agcagaagca gctgctgcgc ccgctgttct ccaacgagca
     3961 catctggtgc cagatgttct ccgagcccgg cgcgggatcc gacgtggcgt cgctggccat
     4021 gaaggccgag cgggacggcg acgagtggat cgtcaacggc cagaaggtgt ggacgaccct
     4081 ggcccacatc gcccgctacg gcctggtgat ctgccgcacc gatcccgacc tgcccaagca
     4141 ccggggcatc accgcgttca tcgtcgacat ggagctgccc ggcgtcgagg tccggccgct
     4201 gcgtcagatg accggcgagg ccgagttcaa cgaggtgtac ctcaccgacg tgcgcatccc
     4261 cgactcgatg cgggtcgacg aggtcggccg gggctggggc gtgtcgatca ccacgctcat
     4321 gaacgagcgg gtgtcgatcg gcggcggcac cccgccccgg ggatcgggcc cgatcggtca
     4381 gctgctcgag gagtggaagc gctccggctc cgacgacccg gtcacccgcg acgcggtcac
     4441 ccagctgtgg atccaggccg aggtcaaccg actgaccaac ctgcgcgccg ccgccaaccg
     4501 caaggcgggc actcccggcc ccgaggggtc ggtggccaag ctcgccatgg ccgagctgaa
     4561 caagaagatc ttctcgctcg cggtcgacat catgggaccc gaggggatgc tgtacggcag
     4621 ctacgagttc gtgcgtcccc gccacgccct cttcaccgac gacatgcaca agaacttcct
     4681 gcgggcccgg gccaactcga tcgagggcgg aacctccgag gtgctgcgca acatcctggg
     4741 cgagcgggtg ctcggcctgc ccggcgacgt acgggtcgac aaggacctgc cctgggcgca
     4801 ggtccccagg tcctgacgac cggtccgggc aactccaccg gttcgaagat ctcccaccga
     4861 gccccgttcg gaaccggatg catgaccgga cggtcacaga cgtagcgtgg agccatgccc
     4921 cgccgtacca agatcgtcgc cacgatcggc cccgcgtcgg actcgcccga cgtgctgcgc
     4981 cgcctgatcg aggtcggcgc cgacgtcatc cgcatcggac tcgcccacgg caccctcgac
     5041 gacgccatgg cccgctaccg caccgtcagg gcggtgtccg aggagctcgg ccgtcacatc
     5101 ggggtgctga tcgacctgcc gggacccaag gtccgcctcg gctcgttcgg cgacagcccg
     5161 accatcctcg acaccggagc gccgctcacc ctcatcccgg gccgtgacac ctccgacacg
     5221 tcggtcctcg gcgtcgacta ccacaacctc ctcaaggaca tccaccccgg cgaccacctc
     5281 ccggtgggcg atggcagcgt cgtcatccag gtcgaatcgg tcgacggcga cgccgccaag
     5341 gccaccgtcc tgtacggcgg gccggtccag ggccgtcccg gactgcacat ctcgtcggac
     5401 cgactcagcc tgtcgacacc gacccccacc gacctgcacc tgctcgacgc cttcgtcgac
     5461 gagggcgtcg acatggtcgc gctcagcttc gtccgctccg ctcacgacat ccgccgcatc
     5521 ggcaccgaac cgcacccccg cggaccgctc gtcgtcgcca agatcgagac gcgtgccgcg
     5581 gtcgagaacc tccaggggat catcgaggcc tccggggcga tcatgatcgc ccgcggcgac
     5641 ctgggcaccg agctgggcat cgaggagctc ccgcacctgc agaagcagat cacccgggac
     5701 tgcatcagcg gcggcaagcc ggtgatcacc gccacccaga tgttcgagtc gatgatcacc
     5761 tcaccgacgc cgacgcgcgc cgaggtctcc gacgtcgcca acgccatctt cgacggcacc
     5821 tcggcggtca tgctctcggc cgagagcgct gtcgggatcg acccggtcaa cgcggtggcc
     5881 accatgagcc gcgtcgccga gcgggccgac caggagttcg actacgacgc ctgggcgcgc
     5941 cggatccgcc acctgcggcg ctccgcggtc ggcaacagct ccgaggacaa gctcaccgac
     6001 gcgatgaccg cagcggcgtg gcgcgcggct accgagatgg gcgtcgccgc catcatctgc
     6061 atcagcgaca gcggcctgac cgtgcgctcg accgcccggt tccgtccggc catgccgctc
     6121 atcggcttca gccccgacga ccggacgctg cgccagctgt cgctcagctg ggggaccacg
     6181 acgttgcgcg ccccccgcca cgtcgacacg atcgccctga tggacgacct gatcgtgcag
     6241 gcccgcgacg ccgggctggt ccgtcccggc cagaccgtcg ccgtgctcgc cggggcgggt
     6301 tcacggtcgc gctccaccga cgtgctgcac ctggcccgcg tgccctgagc ccggtgagca
     6361 gtcccgatcc ggcgccgccc gcgccgctga gccacgacgt cgtcgatggc ctcggcgtga
     6421 tccggcgccg cccacccggc ggcgatccga gccgtcccgc cgtcgtcctc gtccacgggg
     6481 cgatggaccg cgccgccagc ttcgggcggg tgatgcgccg cctcggcgac ctcgacgtgt
     6541 gcgcctacga ccgtcggggc tacgccacct cgctctccgc cgggaccgcg gacgacctcg
     6601 acgcccaggc cgcggatctg caccgcgtcg cccgctggac cggcgcggat cgggtcgtgg
     6661 tcgtcggcca cagcctgggc ggaacggttg cgctgcggtg ggccgaggga gcgccggacg
     6721 gcctcgtctc cctgggcgcc ttcgagtcac cggtgccgac cctcgacaac taccgctccg
     6781 gcggcgcgga cgtcgctctg gccgctgacc gcaccggcgg gccacgcgcc gcggccgagg
     6841 cgttctaccg cttcgtggtc ggggatcgga cctgggacgg cctgcgcgaa cgcgaccgtg
     6901 agacccgacg ggccgagggc gcgcagctgg tggccgagct gcgcgatctg cagcgcccgg
     6961 acggctcacc ccgcctggac gcggtggcgg tcccggtcgt cgtcggcctc ggcggtgcca
     7021 gcgtgggcgc ctggcgggcc gcggccgagg agctggccga acggctgccg agggcgaccc
     7081 gcttcgacat cgctggcgcc ggccacggtg cccacctgtc tcatcccgac gagttcgccc
     7141 gctacgtgcg agccgtgctg gacgcggccg atgacgcggg ccgtgagccc gtagcctctg
     7201 cgccgtggcc accctgaagt cgctcacccg acgcaagccc cgcgtcgcgt tcgtgctgtc
     7261 cgggggcggg aacctgggtt cgttgcaggt cggcatgctc aaggccctca ccgagcacgg
     7321 catcgtcccc gacgtcgtcc tcggctgctc ggtcggcgcc atgaacggcg cggcgtacgc
     7381 gctcgacccc accggtgccg gcatgacccg cctggaggag cactggaggg gggcgttcga
     7441 ggaggtcatg ccgtcgtcgc gggtgccgtc gatgcttcag ctgatccgca agggcgagtc
     7501 gctgcacgcc gccgacggcc tgcggcgcgg cctcgaggtg ctgctcggcg ccacccgccg
     7561 tttcgacgag ctgaccgttc cgttccagtg cgtcgcggcc gagatcgaga ccgcggccga
     7621 gcactggttc gaggacggct acctggtccc ggcggtgctg gcctccgccg cgctgccggc
     7681 cgtgttcccg gccgtgaccg tcgagggccg ccgctacctc gacggtggcg tggtcgacaa
     7741 cgtgcccatc tcccgggccg tcgagctggg ttgccgcgag atctacgtcc tgcacgtcgg
     7801 ccgccacggc aaacccgacg ccgagatccg ccggccgatc gacgcggcgc tccaggcgta
     7861 ctgggtggcg cgtaacgggc gcttcgcccg agacctggcc gccctgcccg acggggtcga
     7921 agcggtcgtg ctgccgccgg ggcagcgacc cgacatccgc tatgacgact tctcccacac
     7981 tgccgagctg atcgaccgcg gctacgagaa ctcgatgacg ctgctggctg aacgccaggc
     8041 gctcgacgcg gagcgtcgga tccgcagcga cgtgctgcag cccctggagc gctggatgca
     8101 ctcggcccgc aaccgcacct ggcgccgcgc cgcccaggac gtggcggtcg accccggagg
     8161 cggcatcctc accgcccccg atcgctccgc cgggttcgac gagaccgacg gtggcgacct
     8221 cgccgatgat gggggagagg tgacctcgcc tgtccgtccc ggctccgggg cagccgggcc
     8281 cggaaagacc tgatcctcga cgaccgggag cgggtgcgga tctgaccgtg gcgcgtcgag
     8341 cgccgctcag ggctccgggc tgaagtggtc agggtcgagc tgtgccagga agtgccggca
     8401 gcgctcctcc tcgtcgacct ctccgatttg cgccgccatg gctcgcagcc cggcgaggga
     8461 ccgcaggaac ccccggttgg tggggtgcgc ccaccgcacg tagccgctgc cccgccagcc
     8521 cgaggcccgg agccggtcca gcccgcggtg gtagccgatg cggtagtagg cgtacgactc
     8581 gacgtcgtca cggccccggt cacccagctc ggcccacgcc tgcaggcacc gggggttcgc
     8641 cgccaccacg tcggccacgg cgcggcgctg ctcgtcggtc gggcgggaca gagcggcctc
     8701 gagggcggac tcggtcgccg ccggctcggg gtcgaggatc gtctcgggcg gtcccgacgg
     8761 gttgaggttg acaggtcgat cggtcatcgg gggtcaggat cccacgacga cggccccgac
     8821 gggagtcgcg agccacttcg caccgaggat ctccctgact ggattcgacg gcaacatcac
     8881 cggtcctcag gaggtcgaag tggcagtcga cgcacccgga tccggtcatc gtcgagcacg
     8941 acgattgctc cggcgtccag gtcgtcggcg accagctgct ccgccagcag cggggacggg
     9001 ttctggtcga gcagcagcct cacgcgggac gcagcagcgg gagctctctc tcctggaccg
     9061 cgagagctgc gtagcggagc gactcgacga cgtcctcggc ctcgagatcg ggatgctcag
     9121 ccaggatctc gtcggttgac atgccatccg ccaccatcgc aacgatggag gccacaggaa
     9181 atcgcaggtc acgcacgaag ggcacgccgc ccatacgctt cgggtccacg gacactcgat
     9241 cgaaccgcac ccgcccagac taccggggaa gcgagacgtc gcagcgggcg tcgaacgcag
     9301 caccaccggg cggaccgacc tcacaggtcg ttggtacgac cttgcggtcg gcggccccgt
     9361 tgggagggat ccggggtgtc gtcagctggc ttcgagatgg tggcggagcg cgtcccggat
     9421 gacctcggag acggccacac cgtcagcctc cgcccgacgg acgagtcgct ccttgagctc
     9481 gggatcgagt cgaaccgact ccaccgtcga gggttctgac ccgagccgag gccgcccacg
     9541 ccgcccgcgt cgtgcgacca ggcgctcgac gtcgtagccc tgctcggctt cgtccgcgaa
     9601 ctgctcgacg agctgatccg acacgggacc gcccggaccg gcgctcgagc tgtgagttga
     9661 ggtggtcatc gttcgagcag cctccggtag atcgggcgca tggccatggc gtggatgatc
     9721 atctcggaac cgtcggcgct gacgagaacg acgacctcca gcagattgcc ggatcggtcg
     9781 ggcccgagca cgagccaccg gtccgggtcc tccccgacgt cctcgacagc caaggagtgc
     9841 tcgagcgcat ggagcatctc gtcgtcagcg atgccgtgac gtcgggctgc tcagtggatc
     9901 tccacacggg attactgtac tacgtaattg cgaaggtggg cgtgtgagcc cggcacatga
     9961 ccgaaccgag gctgacctcc acggacgcca gcagactcga cggaccagcc ccacggtcgt
    10021 gtgcgagaac gacccctgtg cagtcgttct cgcacacgaa gaccaccaac ctcacaagtc
    10081 gccggtacga ccatcctgga tcgccagctc gcagccagag ccggggtggc ggtccagtcc
    10141 gtcggcggag gtcgtgtgga gggcaccgac gcgatccatg gtcgtcagaa gcaggctggc
    10201 gtcggcgacg accgccgtct tctcgaaccg acttggccgc gcgggcggat cgacggtttc
    10261 gggtgccggt gcgaccagcg ccggtcgtac acttccgtca tggcctacga gaggatcacg
    10321 gtcgacccgg cccgcatggg cggtgtgccg accatccgtg acactcgtgt caccgttggg
    10381 atggtgctgg gccaactcgc tgcaggtcgc acggtcgacg acgtgctcgc cgactatccg
    10441 taccttgaac gcgacgacat ctggggtgcg ctcgagttcg ccgccgcagc cgtcaacgag
    10501 cgcgaggttc ctgtcgcccg tccggcatga ggttgctcat cgacgccaac ctgtcgccgc
    10561 gggtcgcaga agggcttcgc tctgccggct acgacgcagg ccacgtcgcc gacttggaca
    10621 tggtgaccgc cacggacgaa cagatcttcg accgcgccgt cgccgacgac ctcgtcgtcg
    10681 tgacggctga cagcgacttc gggaagctgc tggcgttgcg ccgggcatcg aacccgtcgg
    10741 tgatccacgt tcgcggagtc gcagagctcg caccggaggt ccacctcgac ctcctcgtcc
    10801 ggaatctgcc caccatcgcc ggtgacctcc gtcgcggcgt gatcgcttcg ctcagccccg
    10861 cacggctcgc tctccgcgac ctccctctcc gctgaacctg ggaccgaccg cgctgccggg
    10921 ccggctctgt ggacccgggc ctgccggact cagtcgatac cgcaccagac gttcacggcg
    10981 acactcgacg gaccaacccc acgttcgcgt gcgaggacga cccctgtgca gtcgtcctcg
    11041 cacacgaacg ccaccaac
//
LOCUS       DAOQOD010000104        10824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2171_length_10824_cov_12.083759, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000104 DAOQOD010000000
VERSION     DAOQOD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_10824_cov_12.083759"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..817)
                     /gene="npdG"
                     /locus_tag="PLZ72_10625"
     CDS             complement(161..817)
                     /gene="npdG"
                     /locus_tag="PLZ72_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNA36793.1"
                     /translation="MEIGVLGGTGPAGSGLAVRLASVGFDVVIGSRSKYRAMEVADKL
                     LAKWSDRELSIAAGDNHTAASAEVVFVATPWDAAAATVKDCETELKGKVVISMANALA
                     RVGHEFQPLVPPRGSVAASVQAAVPRSLVAASMHHVPAAELGDIAHPVESDVLVCSDH
                     PSATATTIDIVSRIPDLRAVDAGELSNAAPIEAFAAVLLQVNVKYKTRVAVRFTGLPA
                     "
     gene            complement(830..1582)
                     /locus_tag="PLZ72_10630"
     CDS             complement(830..1582)
                     /locus_tag="PLZ72_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36794.1"
                     /translation="MTRSDTPRVTIVSPHFDDAPLSLGQSLLDGALHGCRVRVRVVFA
                     RTNFTRWVHPTRGRAGPISLWRRAEESVAQVAFGYRVATSPHEEVVLRTGELDPAVLL
                     DPTSELDDALVDTLTAQARRWRGDGSTVLFPAGIGGHLDHRLVAEAGRRLLAEHPDRI
                     GFYEDRPYASLVDPGVVEETLRAIDPTLVPVDVSGPVTERLHRRLRRCYPSQIDDLFV
                     RAMDADRATGACERVWFAPGQVPAWLSFRDGR"
     gene            complement(1579..3012)
                     /locus_tag="PLZ72_10635"
     CDS             complement(1579..3012)
                     /locus_tag="PLZ72_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA36795.1"
                     /translation="MDDSTSKGATGRRSTSELAEGLAEGLASVVGSALGVGVRVEGLT
                     RLSGGASRETWSFVVVPADGGPDRRLVLQRERAGNAATSQAMAVQAELLAAAVAAGVP
                     VPTVVVADPDGVSLGAPAVVMDHVDGETIARRILRDDEYAAARDVLVAQAGAALAAVH
                     RMDDAAVAATEAEDPVAQMRNLLDAFGEPHPAFELGLRWLERNRPAPRPATVVHGDFR
                     MGNLIVGPDGIRAVLDWELAHRGDPIEDLGWFCVRAWRFGSPAVAGGVGSVQQLIDAY
                     VAAGGAPVTVDEVRWWEALGTLRWGLICILQASVHLGGASRSVELAAIGRRTAENEED
                     LLEIVAGPSDRVPAPAARPSVEVGAAPHDRPTAAELLDAVEEYLTEVRDGVPGRLGFH
                     ARVAANVVATVRREIELGPDQARLLDERLASLGISATGRAGEAELAAAIRRGDLDDRV
                     DEVTDAVRATVRDKLAVSNPGYWERPL"
     gene            complement(3108..4169)
                     /locus_tag="PLZ72_10640"
     CDS             complement(3108..4169)
                     /locus_tag="PLZ72_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA36796.1"
                     /translation="MSWYGVTLLGAALIVGNAFFVAVEFSLLAARRQRIEQWVDDGRF
                     GAAAALAQMRSLNLQLATCQLGITVLSLLLGWLIEPAIGSVIEGWLEHSPLPEATGRI
                     IGVAVALMIVAFLHMVLGEMVPKSVALTAPERSAVLLAPLQAVFTFVLWPFVWVLDRL
                     SRWGTRLLRVEPADELAQAHTSAELAIMMEESVASGELEATEHVLLAGALGFLDRRVA
                     EVMAPASQLVTVPTTATVARAEELVHRSGHSRVLVVGSSRDDVVGFLHAKDLLRIPTD
                     RRDGLLPPGVVRVALRVRPDDRLSDVLPRMRRARRHVAVVADRDDRGVERVVGLVTLE
                     DVLEAIVGDIRDEWDRPGT"
     gene            complement(4166..5542)
                     /locus_tag="PLZ72_10645"
     CDS             complement(4166..5542)
                     /locus_tag="PLZ72_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNA36797.1"
                     /translation="MSIAVGLIAVLALVLANAVFVAAEFSLVAVDRSQVTIQVEAGSR
                     SARRVDAILRRLSFALSGAQLGITLCSLALGVLSEPVIAPLLEPLFGPLDARAAVTAS
                     VIAALLVTTVVQMVVGELIPKGVAVARPLSTSLAMAVPFGLFVTLFRPVIVVGDAAAD
                     ALLRLVGLEPAEELSTVRSRDELHRLVHTSQEGGTLVGPLAELLDRSFRFRSKTAADA
                     LTPRTDITALRSDATTGDLLDASERTGLSRFPVTGDDLDDITGVVHIKDVLGIAPSQR
                     RSTPLADVVRPVVMVPESTELGDLLVQLQDESGQFAVVLDEYGATAGIITLEDLVEEI
                     VGDIADEHDPTTVPRVRRWQGAHLVSGRLHPDEVVEACGFEVPDGPYETLAGYVLNVL
                     GSVPEVGDGFSDRGWSVEVAEMDQRRVAVVRLVAPPPGWLPESGDGDAARTRDGNGGR
                     PDDGSGSS"
     gene            5611..6072
                     /locus_tag="PLZ72_10650"
     CDS             5611..6072
                     /locus_tag="PLZ72_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA36798.1"
                     /translation="MSTPDPPEDSRCADAARAAARVARVAANAVAEVDLTLAQYRVLV
                     FVDGVDRPATEVARLLGVSPSTLTSVVDGLCGRDLVQRLSDPADGRRVVLSITDDGRA
                     RVARADAAVADRLSGLLARLGPERASAALDGLDLLNEAMDDYLREHFGGHR"
     gene            6069..6854
                     /locus_tag="PLZ72_10655"
     CDS             6069..6854
                     /locus_tag="PLZ72_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36799.1"
                     /translation="MSVPALAMVGVGFTRDGRDLLDGVDWRVEADQDWVVIGLNGSGK
                     TSLIRIAALYEHPSRGTVDVLGERLGRTDVRVLRTRLSLVSAAMADLVRPNLSAAEVV
                     MCGRFAALEPWWHTYTEADRDRARALLATQGVGAVADHPMGTLSSGERQRTLLARALM
                     NEPGLVLLDEPTAGLDLRGREELVDRLDALAVNPDAAPLVLVTHHVEEIPPGFTHLLA
                     LRDGRVLAQGPVDDVLTAELLSDTFGLRLVLERRDGRFFARRS"
     gene            complement(6939..9068)
                     /gene="glgX"
                     /locus_tag="PLZ72_10660"
     CDS             complement(6939..9068)
                     /gene="glgX"
                     /locus_tag="PLZ72_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNA36800.1"
                     /translation="MIWAGGKLRVWPGRPAPLGAHWDGAGTNFAVFSGSAANEGGAPD
                     EHGGVTLCLFDEDDAEIRIPLPERAGSVWHGYLPDVGPGVRYGYRVTGPRLRPGDLQD
                     PSKLLLDPYARALTGSLRWTDSVQPASGDGSAADSAPDMPRSVVVNPWFDWGQDRRPN
                     TPWHRSVLYEAHVRGMTQRHPDVAPELRGTYLGLCSSPIIEHLLALGVTAVELMPVHQ
                     FVHDRRLIEAGLRNYWGYNSIGFFAPHDTYGVEGGGRGAHVQEFKVMVKTLHEAGIEV
                     ILDVVYNHTAEGGAGGPVLSFKGLDNPGYYRLDPEDPSAYVDYTGTGNSLNMRHPNVL
                     QLIMDSLRYWVQDMHVDGFRFDLAATLARELHDVDRLSSFFDLIQQDPVVSQVKLIAE
                     PWDVGEGGYQVGNFPALWSEWNGRYRDCVRDLWCRQQGVLGEFASRLTGSSDLYQDDG
                     RRPHASINFVTAHDGFTLADLVSYEHKRNEANGEDNRDGTDDNRSTNCGVEGPTDDPV
                     VLAHRDRLRRNLMATLMLSQGVPMLLHGDEIGRTQGGNNNAYAQDNEVSWIDWSAVDA
                     EFLEFCRGLIALRRRHPALQRHRFLTGRPVAGDTRPDVAWFTPAGVEMTWEDWGHDAR
                     QVAMWLNGDLAETGPRGEVIVDDTLLVCINASDQPSAQVLPDERWAAAWTLVLDTASA
                     AAPPSTRYPAGGTVQMGPRTILFLERR"
     gene            complement(9109..9594)
                     /locus_tag="PLZ72_10665"
     CDS             complement(9109..9594)
                     /locus_tag="PLZ72_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA36801.1"
                     /translation="MDVTATLAADVPIDALFEVVADLGTYPEWLDIVGRAEPLDPATG
                     ADGTAEDAAWSVDLKGQVGPFRRSKRLRMVRACFDDPDATGRASVRFERRELDGRSHS
                     DWVLSAELSSPAGDGAPELRMRLHYGGNLWVPLLDRILTDEIERSRPRLVAFVEGRAG
                     S"
     gene            complement(9630..10040)
                     /locus_tag="PLZ72_10670"
     CDS             complement(9630..10040)
                     /locus_tag="PLZ72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA36802.1"
                     /translation="MPISTGFNHVATLTTDMDLTVGFYEQVFDAEVTFEVARTDDHPW
                     MKIVDIGGGAALNIFEVDPSEIIGERRRQGGRGAIDHFALAVDSPAVLEQMRDRLLDS
                     HAQEVGEIQQLGHELSLFFRDPDGMDLEVCCPAD"
     gene            10164..10520
                     /locus_tag="PLZ72_10675"
     CDS             10164..10520
                     /locus_tag="PLZ72_10675"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNA36803.1"
                     /translation="MIEALPDMPDGVLGFEAVGEVHAEDYVTTLVPAIEAHSGPVRLV
                     YVLGERFDGYSSGAMWQDAKLPLQHHEQWHRLALVTDVDWVRHLAAVFGWMIPGKFEV
                     FPTDRLTDAIAWVAED"
     gene            complement(10533..>10824)
                     /locus_tag="PLZ72_10680"
     CDS             complement(10533..>10824)
                     /locus_tag="PLZ72_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36804.1"
                     /translation="RRWWRVEMPMVRGAVVAGAGLAFVASLGEFGATVFLARADRPTV
                     PVAIERLMSRPGSAGFGQAMALSCVLVLLCGAVLAVIDRSVDAFTGSRSDPS"
BASE COUNT         1614 a         3929 c         3831 g         1450 t
ORIGIN      
        1 ccatgacttg gttcatttcc aagccgacct ccgcggaaga ctcagacaca cctcagccca
       61 ccgacgaccc gacgacccca gagctacaac aacgacagcg cccaccgaac ttgtagccca
      121 ccgacgcccc tgcgacccga gggctacaac aacgcctact tcaggccggc aggccggtga
      181 accgcaccgc gacgcgggtc ttgtacttca cgttcacctg gagcagcacg gccgcgaacg
      241 cctcgatcgg tgcggcgttg gacagctcgc cggcgtcgac ggcacgcagg tcggggatgc
      301 gcgacacgat gtcgatcgtg gtcgccgtcg cggacgggtg gtccgagcac accagcacgt
      361 cggactcgac cggatgggcg atgtcgccga gctcggccgc cggcacgtgg tgcatgctcg
      421 cggcgaccag tgaccggggc accgcggcct ggaccgacgc ggcgaccgac ccccgaggcg
      481 gcaccagcgg ctggaactcg tgaccgaccc gggccagggc gttcgccatc gagatgacga
      541 ccttgccctt caactcggtc tcgcagtcct tcaccgtcgc cgctgccgcg tcccacgggg
      601 tggccacgaa cacgacctcg gccgacgccg cggtgtgatt gtcacccgcc gcgatcgaca
      661 gctcccggtc ggaccacttg gccagcagct tgtcggccac ctccatggcc cggtacttgg
      721 acctcgaccc gatcacgacg tcgaagccca ccgacgccaa ccgcaccgcc aggccgctgc
      781 cggccggacc cgttcctccg aggacgccga tctccatgga ggcagtgtgc tagcgaccgt
      841 cgcggaatga caaccaggcc ggcacctgac cgggagcgaa ccacacgcgc tcgcacgctc
      901 cggtggcccg gtcggcgtcc atcgcccgca cgaacaggtc gtcgatctgg ctcgggtagc
      961 agcggcgcag ccgacggtgc agccgctcgg tcaccggacc cgacacgtcc accgggacca
     1021 acgtcgggtc gatggcccgc agcgtctcct cgaccacacc cggatcgacc aacgaggcgt
     1081 acggacggtc ctcgtagaag ccgatccggt ccgggtgctc ggcgaggagc cgacgcccgg
     1141 cctcggccac cagacgatgg tcgaggtggc cgccgatgcc cgccgggaac agcaccgtcg
     1201 acccgtcgcc gcgccagcgt cgggcctgag cggtcagggt gtccaccaac gcgtcatcca
     1261 gctcggaggt cgggtcgagc agcaccgccg ggtccagctc gccggtgcgc aacacgacct
     1321 cctcgtgcgg cgacgtggcg acccggtagc cgaaggcgac ctgagcgacc gactcctcgg
     1381 cccggcgcca caggctgatc ggtcccgccc ggccccgggt cggatgcacc cagcgggtga
     1441 agttggtcct ggcgaacacg acccggaccc ggacccggca cccgtggagc gccccgtcga
     1501 gcaggctctg gcccagcgac aggggggcgt cgtcgaagtg cggcgacacg atcgtcaccc
     1561 ggggggtgtc cgagcgggtc acaggggccg ttcccagtag ccggggttgg acaccgcgag
     1621 cttgtcgcgg accgtggccc ggaccgcgtc ggtcacctcg tcgacgcggt cgtcgaggtc
     1681 accccgacgg atcgccgcgg ccagctcggc ctcgccggcg cgtccggtgg ccgagatgcc
     1741 cagcgacgcc agccgctcgt cgagcagacg ggcctgatcg ggtcccagct cgatctcgcg
     1801 gcggaccgtg gctaccacgt tcgcggcgac ccgggcgtgg aaccccaacc gtcccgggac
     1861 gccgtcacgg acctcggtca ggtactcctc gaccgcgtcg agcagctcgg ccgcggtcgg
     1921 acgatcatgc ggtgccgccc ccacctcgac cgagggtcgg gcggccggcg ccggcacccg
     1981 gtcggagggc ccggcgacga tctcgagcag gtcctcctcg ttctcggcgg tccgccgacc
     2041 gatggcggcc agctcgacgg aacggctggc gccacccagg tgcaccgacg cctgcaggat
     2101 gcagatcagc ccccaacgca gcgtgcccag cgcctcccac cagcggacct cgtcgacggt
     2161 cacgggggcg ccgcccgcgg cgacgtacgc gtcgatgagc tgttgcaccg atcccactcc
     2221 cccggcgacg gccggcgagc cgaaccgcca ggcccgcacg cagaaccagc cgaggtcctc
     2281 gatcgggtcg ccccggtgcg cgagctccca gtcgagcacg gcccggatac cgtcggggcc
     2341 gacgatcagg ttgcccatcc ggaagtcgcc gtggaccacc gtggcgggcc ggggtgcggg
     2401 gcggttccgc tccagccacc gcagcccgag ctcgaaggcg gggtgcggtt cgccgaacgc
     2461 gtcgagcagg ttgcgcatct gggcgaccgg gtcctcggct tcggtcgccg cgaccgcggc
     2521 gtcgtccatg cggtgcaccg cggcgagcgc ggcgccggcc tgggcgacca ggacgtcacg
     2581 ggccgcggcg tactcgtcgt cgcgaaggat ccgtcgggcg atcgtctccc cgtcgacgtg
     2641 gtccatcacc accgcaggag cccccaggct caccccgtcg ggatcggcga ccacgacggt
     2701 cggcacgggc actccggcgg cgaccgcggc ggccagcagc tcggcctgca ccgccatcgc
     2761 ctgcgaggtg gccgcgttgc cggcccgctc gcgctgcagc accagtcggc gatcgggccc
     2821 tccgtcggcc gggaccacca cgaaggacca cgtctccctc gacgctcccc ccgacagccg
     2881 ggtcaggccc tcgacccgaa ccccgacgcc gagcgcggat ccgacgaccg acgccagccc
     2941 ttcggccagc ccctcggcca gctcgctcgt cgaccgccgc ccggtcgccc ccttcgatgt
     3001 gctgtcgtcc acgccgtcac cccctgagcc cgatcgggcc gctcgcccac cggttcggat
     3061 cgccttctct accgcaactc gccaccacgc ccacggatcg tgcccggtca ggtgccgggt
     3121 cggtcccact cgtcgcggat gtcgccgacg atcgcctcga ggacgtcctc gagcgtgacc
     3181 agcccgacga cgcgctccac cccccggtcg tcgcggtccg cgaccaccgc cacgtgccga
     3241 cgggcccgac gcatccgcgg cagcacgtcg ctgaggcgat catcgggccg gacccgcaac
     3301 gccacccgca ccacgcccgg gggcagcaga ccgtcgcggc gatcggtggg gatgcgcagc
     3361 aggtccttgg cgtgcaggaa gccgacgacg tcgtcgcggg aggatccgac cacgagcacc
     3421 cgtgagtgcc ccgagcggtg caccagctcc tcggcgcggg ccaccgtcgc ggtcgtcggc
     3481 accgtcacca gctgcgatgc gggcgccatg acctcggcca cgcgccggtc gaggaagccg
     3541 agcgcgccgg ccagcagcac gtgctcggtg gcttcgagct cgccggaggc gaccgactcc
     3601 tccatcatga tcgccagctc ggcggaggtg tgggcctggg caagctcgtc ggccggctcg
     3661 acccgcaaca gccgcgtgcc ccaccgcgac aggcggtcga gaacccagac gaagggccag
     3721 aggacgaagg tgaagaccgc ctggagcggg gcgagcagga ccgccgaccg ctccggggcg
     3781 gtgagagcga ccgacttggg gaccatctcg ccgagcacca tgtgcaggaa ggcgacgatc
     3841 atcagcgcga cggcgacgcc gatgatccga ccggtcgcct cgggcagggg gctgtgctcg
     3901 agccagccct cgatcaccga cccgatcgcc ggttcgatca accagccgag cagcagcgac
     3961 agcaccgtga tgccgagctg gcaggtggcg agctggaggt tgagcgaacg catctgcgcc
     4021 aacgccgcgg cggcgccgaa ccggccgtcg tcgacccact gctcgatgcg ttggcgtcgc
     4081 gctgcgagca gggagaactc gaccgcgacg aagaaggcgt tgccgacgat caacgcggcg
     4141 cccaacaggg tcacgccgta ccagctcacg acgacccgct cccgtcgtca gggcggccgc
     4201 cgttgccgtc tcgggtccgg gcagcgtcgc cgtcgccgct ctcgggcagc cacccaggcg
     4261 gcggggcgac cagtcggacg accgccacgc gccgctggtc catctcggcc acctcgacgg
     4321 accagccccg gtcgctgaag ccgtcgccga cctcgggcac cgagccgagg acgttcaaca
     4381 cgtagccggc gagcgtctcg tacggcccgt cggggacctc gaacccgcag gcctcgacca
     4441 cctcgtcggg gtgcagccga cccgacacca ggtgcgctcc ctgccaacgt cgaacccggg
     4501 gcacggtggt cgggtcgtgc tcgtcggcga tgtcgccgac gatctcctcg acgaggtcct
     4561 ccagggtgat gatcccggcg gtggcgccgt actcgtcgag gacgacggcg aactggccgg
     4621 actcgtcctg gagctgcacc agcaggtcac cgagctcggt cgactcgggc accatcacca
     4681 ccgggcggac cacgtcagcc aacggcgtgc tgcgacgctg cgacggggcg atgccgagga
     4741 cgtctttgat gtggaccaca ccggtgatgt cgtcgaggtc gtcaccggtg acggggaatc
     4801 gggacagccc tgtgcgctcc gacgcgtcga ggaggtcgcc ggtcgtggcg tcggagcgca
     4861 gggcggtgat gtcggtccgc ggggtgaggg cgtcggctgc ggtcttggag cggaaccgga
     4921 agctccggtc gagaagctcg gccagcggcc cgacgagcgt gccgccctcc tgggatgtgt
     4981 gcaccagccg gtgcagctcg tcgcggctgc gcaccgtcga cagctcctcg gccggttcga
     5041 gcccgacgag gcgcaggagc gcgtcggccg cggcgtcgcc gacgacgatg accgggcgga
     5101 acagcgtgac gaacaacccg aagggcaccg ccatggcgag cgacgtggac agcggacggg
     5161 cgaccgccac ccccttgggg atcagctcgc cgaccaccat ctgcaccacg gtggtcacca
     5221 ggagcgcggc gatgaccgac gcggtcaccg cggcccgggc gtcgaggggg ccgaagagcg
     5281 gttcgagcag cggcgcgatc accggttcgg acaggacgcc gagggccagg ctgcacaggg
     5341 tgatgcccag ctgggcgccc gacagggcga acgagagtcg acgcaggatc gcgtccactc
     5401 ggcgggcgga tcgggaacct gcctcgacct ggatcgtgac ctggctgcgg tccacggcga
     5461 cgagcgagaa ctccgccgcg acgaacacgg cgttggccag gaccagggcg agcaccgcga
     5521 tcaggccgac ggcgatgctc acggccgcga ctctaccggc gactgtgtcg acgtcggagc
     5581 cgccgggcgc ggcccggtac ggtgacgccc gtgtcgactc cggatccgcc cgaggactcc
     5641 cgctgcgcgg acgcggcccg cgccgcggcg agggtggccc gcgtcgcggc gaacgcggtg
     5701 gccgaggtcg acctgaccct ggcgcagtac cgggtgctcg tcttcgtcga cggggtcgac
     5761 cgacccgcca ccgaggtggc gcgcctgctc ggcgtgtcgc cgtcgacgtt gacctcggtg
     5821 gtcgacgggc tgtgcgggcg ggacctggtg cagcggttgt cggaccccgc tgacggccgg
     5881 cgtgtggtgc tgtcgatcac cgacgacggc cgggcccggg tcgcccgggc cgacgctgcg
     5941 gtggccgacc gcctgtcggg cctgctcgcc cgcctgggtc ccgagcgggc ctccgctgcg
     6001 ctcgacgggt tggacctgct caacgaggcg atggacgact acctgcgcga gcacttcggc
     6061 ggacaccggt gagcgtcccc gcgctggcca tggtcggcgt gggcttcacc cgcgacggcc
     6121 gcgacctgct cgacggcgtg gactggcgcg tcgaggccga ccaggactgg gtggtgatcg
     6181 gcctcaacgg aagcggcaag acgtcgctga tccgcatcgc cgcgctctac gagcacccga
     6241 gccgcggcac cgtcgacgtg ctcggcgagc ggctcggccg caccgacgtc cgggtgctgc
     6301 gcacccgact cagcctggtc tccgcggcga tggcggacct ggtgcggccc aacctgagcg
     6361 ccgcagaggt ggtcatgtgc ggccgcttcg ccgcgctcga accgtggtgg cacacctaca
     6421 ccgaagcgga ccgggatcgg gctcgggcgc tcctggccac gcagggcgtg ggcgcggtgg
     6481 ccgatcatcc gatgggcacg ctgtcctccg gtgagcgcca gcgcacactg ctggcccggg
     6541 cgctgatgaa cgagccgggc ctcgtgctgc tcgacgagcc gaccgccgga ctggacctgc
     6601 ggggtcgcga ggagctggtc gaccggctcg acgccctggc cgtgaacccg gacgcagcgc
     6661 cgctggtgct ggtcacccac cacgtcgagg agatcccgcc gggcttcacg cacctgttgg
     6721 cgctgcgcga cggtcgggtg ctggctcagg gccctgtcga cgacgtgctg accgcggagc
     6781 tgctgagcga caccttcggc ctgcggctgg tgctcgagcg acgcgacggc cggttcttcg
     6841 cccgccgctc ctgaccccct ccgcccgttc tgttccgccc gttctgttcg ttgttccaca
     6901 acgtcgacgt tgtggaacaa cgaacagaac gggaggggtc agcggcgttc gaggaacagg
     6961 atggtccgcg ggcccatctg caccgtcccg cccgccgggt agcgagtcga cggcggggca
     7021 gcagcggacg cggtgtctag caccagcgtc caggctgcgg cccagcgctc gtcgggcagg
     7081 acctgcgcgg acggctggtc cgaagcgttg atgcacacca gcaacgtgtc gtccacgatc
     7141 acctcgcccc gcgggccggt ctcggccagg tcgccgttga gccacatggc gacctgacga
     7201 gcgtcgtgac cccagtcctc ccatgtcatc tcgacgccgg ccggggtgaa ccacgccacg
     7261 tcgggtcggg tgtcgccggc gacgggacgg ccggtgagga acctgtggcg ctgcagggcc
     7321 gggtgccgtc gacgcagcgc gatcagcccg cgacagaact cgaggaactc cgcgtcgacc
     7381 gccgaccagt cgatccacga cacctcgttg tcctgggcgt acgcgttgtt gttgcccccc
     7441 tgggtgcggc cgatctcatc gccgtgcagc agcatcggca cgccctggga gagcatgagc
     7501 gtggccatca ggttccggcg cagacggtcc cggtgggcga ggacgaccgg gtcatcggtc
     7561 ggaccctcga ccccgcagtt ggtggagcgg ttgtcgtcgg tgccgtcgcg gttgtcctcg
     7621 ccgttggcct cgtttcgctt gtgctcgtag gagaccaggt ccgccagggt gaacccgtcg
     7681 tgagcggtga cgaagttgat gctggcgtgg ggtcgccgac cgtcgtcctg gtagaggtcc
     7741 gagctgccgg tcagccgaga cgcgaactcg ccgagcaccc cctgctggcg acaccacagg
     7801 tcccggacgc agtcgcggta gcggccgttc cactccgacc acaaggccgg gaagttgccg
     7861 acctggtagc ccccttcccc cacgtcccac ggctccgcga tcagcttgac ctggctgacc
     7921 accggatcct gctggatcag gtcgaagaag ctcgacagcc ggtcgacgtc gtgcagctcg
     7981 cgggccagcg tggcggccag gtcgaagcgg aacccgtcga cgtgcatgtc ctgcacccag
     8041 taccgcaggc tgtccatgat cagttgcagc acgttggggt ggcgcatgtt caagctgttg
     8101 ccggtgccgg tgtagtcgac gtacgcggag gggtcctcgg ggtcgaggcg gtagtagccg
     8161 gggttgtcga ggcccttgaa gctgagcacg ggtccgccgg cgcccccctc ggcggtgtgg
     8221 ttgtagacga cgtcgaggat cacctcgatg ccggcttcgt gcagggtctt gaccatgacc
     8281 ttgaactcct gcacgtgggc gccccgcccg ccgccctcga cgccgtaggt gtcgtgcggc
     8341 gcgaagaacc cgatcgagtt gtagccccag tagttgcgca gaccggcctc gatcaggcgc
     8401 cggtcgtgga cgaactggtg caccggcatc aactcgacag cggtgacgcc cagggcgagc
     8461 aggtgctcga tgatcggcga cgagcacagg cccaggtagg tgccgcgcag ctcgggggcg
     8521 acgtcggggt gccgctgggt catgccgcgg acgtgggcct cgtagagcac gctgcgatgc
     8581 cacggcgtgt tcggacgacg gtcctggccc cagtcgaacc acgggttgac gaccaccgac
     8641 cggggcatgt cgggagccga gtccgctgcc gacccgtcgc ccgaggccgg ctggaccgag
     8701 tcggtccagc gcagtgagcc ggtcaacgcc ctggcgtacg ggtcgagcag gagcttggac
     8761 gggtcctgga ggtcgccggg acgcaggcgt ggtccggtga cgcggtaccc gtagcggacc
     8821 ccggggccga cgtcgggcag gtagccgtgc cacaccgaac ccgcccgctc gggcagcggg
     8881 atccggatct cggcatcgtc ctcgtcgaac aggcagagag tcaccccgcc gtgctcgtcg
     8941 ggggctccac cctcgttggc ggcggaaccc gagaacaccg cgaagttggt gccggcgccg
     9001 tcccagtgcg ccccgagcgg agctggtcgg ccgggccaca cccgcagttt gccgccggcc
     9061 cagatcaccg gtacggcctc ggcgccggcc cgtgagcgct cccgtcgctc acgagcccgc
     9121 ccgaccctcg acgaaggcga ccagccgggg ccgggatcgc tcgatctcgt cggtcaggat
     9181 ccggtcgagc agcgggaccc acaggttgcc gccgtagtgc aaccgcatgc gcagctccgg
     9241 cgcgccgtcc ccggccgggg agctgagctc agcggagagc acccagtcgc tgtgggatcg
     9301 cccgtcgagc tcgcgccgtt cgaaccgcac gctcgcgcgg cccgtggcgt cggggtcgtc
     9361 gaagcacgcc cggaccatcc gcagccgctt ggaccgacga aacgggccga cctgaccttt
     9421 gaggtcgacc gaccaggccg cgtcctcggc ggtcccgtct gcaccggtgg ccgggtcaag
     9481 tggttcggcc ctcccgacga tgtccaacca ctccgggtac gtgcccaggt ccgcgacgac
     9541 ctcgaacagg gcgtcgatcg gcacgtcggc ggcgagggtg gcggtcacgt ccacgcccga
     9601 cagtctgccc ggagatcgaa ccgggccggt cagtcggccg ggcagcacac ctcgaggtcc
     9661 atgccgtcgg ggtcccggaa gaacagcgac agctcgtggc cgagctgttg gatctccccc
     9721 acctcctggg cgtgggagtc gaggaggcgg tcgcgcatct gctcgaggac ggccggtgag
     9781 tcgaccgcca gagcgaagtg gtcgatggcg ccccgtccgc cctgtcgacg gcgttcaccg
     9841 atgatctcgg acgggtccac ctcgaagatg ttgagagcgg ctcccccgcc gatgtcgacg
     9901 atcttcatcc acgggtggtc gtcggtgcgg gccacctcga aggtgacctc agcatcgaac
     9961 acctgctcgt agaagccgac ggtgagatcc atgtcggtgg tgagtgtggc cacgtggttg
    10021 aaaccggtcg agatgggcat ggtggtcctc cgagctgccg gtccgctcgt gcagcctatt
    10081 gaaccgaccc accgaatatc ggggtccgaa tcgacatagc ctgtcgattc cgagccccag
    10141 attcgccgag gaacggagca agcgtgatcg aagcactgcc cgacatgccc gacggcgtcc
    10201 tcggcttcga ggccgtcgga gaggtccacg ccgaggacta cgtcacgacg ctcgtgccgg
    10261 cgatcgaggc ccactccggt ccggtccgcc tggtctacgt gctcggcgag cgcttcgacg
    10321 gctacagctc gggggcgatg tggcaggacg ccaagctgcc gttgcagcac cacgagcagt
    10381 ggcaccgact cgcgctggtc accgacgtgg actgggtccg ccacctcgcg gccgtgttcg
    10441 ggtggatgat cccgggcaag ttcgaggtgt tcccgaccga tcggctcacc gacgcgatcg
    10501 cctgggtggc cgaggactga cgtcagatcg cctcagctgg ggtcggagcg gctcccggtg
    10561 aaggcatcga cgctccggtc gatcacggcc aggaccgccc cgcagagcag gacgagcacg
    10621 cagctcaacg ccatcgcctg accgaatccg gccgagccgg gccgcgacat cagacgctcg
    10681 atggccaccg ggaccgtcgg tcggtcggcc cgggccagga acaccgtggc gccgaactcg
    10741 cccaggctgg ccacgaaggc cagccctgct ccggcgacga cggcgccgcg caccatcggc
    10801 atctcgaccc gccaccaccg ccgg
//
LOCUS       DAOQOD010000105        10735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2200_length_10735_cov_10.968539, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000105 DAOQOD010000000
VERSION     DAOQOD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2200_length_10735_cov_10.968539"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PLZ72_10685"
     CDS             <1..1327
                     /locus_tag="PLZ72_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36805.1"
                     /translation="MARSAAGHTGAEKRAGWSFLAVSVNNSLYTLNAFRPSNRFRLLF
                     GWSFFASWVTIELAPYILAVEVLLGAWFVKRGALRTAPGRAGMVLTLGTCVGMAATIR
                     QSFRARHEIRQALRDVVEVDRADPLPVRWERRVEFARAGGRSLRMDILRPDVADSADV
                     ADGGLPGRPVLVQVHGGGWVLGFKERQGQLLMREMAARGWVCCNVDYRLSPTATFPDH
                     LVDVKRAIAWIRDNADELGADPGFVAVTGGSAGGHLTALTALTANQERYQPGFEGADT
                     SVVAAVPFYGVYDFTDRNGVWPPDTQSMFLAPVVMKCDPDERPDLWADASPIDQVHAG
                     APTFMVIHGDRDVLAPVEDARTFVAALRDVSEQPVYYLELKGAQHAFETFASVRANAV
                     IAAVGRFLDGVHHAYVTGPGWVAEPDEVREAVEERLGDRITDPQPISGS"
     gene            complement(1376..>2276)
                     /gene="surE"
                     /locus_tag="PLZ72_10690"
     CDS             complement(1376..>2276)
                     /gene="surE"
                     /locus_tag="PLZ72_10690"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNA36806.1"
                     /translation="MRILVTNDDGIDSEGIHELARALAGTGHEIVVVAPSSDWSGAGA
                     ALGNLRPDSALGVASARIPDGDGIEAWAFDGPPALCVVAGRLGAFGELPDLVVSGINA
                     GANTGRAILHSGTVGAALAGQNFGVSGLAVSVDAAAGGGPGAEGWRWDTAASIAVETL
                     PLVVGSPVGSVLNLNVPALHRDQVKGVRWTHLAPFGAVRATVSSRDDQSLQWELTPTG
                     YEPPEDTDQGALQRGWASLTALVGVAESWPAPPDSGVVGRHDDLDVAARIVPGAELHE
                     VHRVPGGSLEGTLVRPHLGDAYL"
     assembly_gap    2277..2376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2377..2966
                     /locus_tag="PLZ72_10695"
     CDS             <2377..2966
                     /locus_tag="PLZ72_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36807.1"
                     /translation="VADPAGREVLSWLVVASLLEAHAELGVVETHPGYDGEYDCLSVY
                     DRTRLDRGPLLDLDRNGVAHIAPLAGGADTWPDVWDRCAEDGPVVVAGGLRARVGLGA
                     LCPHGGRESHTVSQIARRLLKLHLSEIPGTWSCRNGIADSSRGPERRLGLFAEVPAGM
                     ERLAAAPTHPLGDPAYAFWFLLRDGVPISCLDTFH"
     gene            3048..4916
                     /locus_tag="PLZ72_10700"
     CDS             3048..4916
                     /locus_tag="PLZ72_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36808.1"
                     /translation="MSTTSFPLSTTPPLRDLWRHAARRRPTVVAATVLSTANKLFDVL
                     PELLIGVAVDVVVNEGESMVGRLTGVQDRFGQLVILAIVTVVIWVCESATDYAAHRTW
                     RNLAQAVEHDTRMEAYRHIQSLEMAYFEDTTSGGLMTILNDDVNQLERFLDVGADRII
                     QSIANVVLVGAVFAVISPTLMVLAFLPIPVIVVGSLRYQKRLEPRYDAVRAAAADIGH
                     TLSNSLGGVATVKAFSGEEREVARLEAESSNYRETNGRAIALSSAFIPLIRMAILAGF
                     TMTLLVGGKAALDGNLAVGMYSVLVFMTQRLLWPLTELGEILDLYQRAMASCRRIFGL
                     LEMEPRIRPGSRDLPVPVRGDVRFDDVTFSYHRAAPVPAGTAPDEEAPGPPPGSDDAD
                     RNVLRGFSLHVPAGETHAIVGSTGSGKSTVLKLLLRLYEPDSGTVTLDDIPVAELTFA
                     SLRGSTGLVPQEVFLFHGSVADNIAYGRPDATRDQIVAAAELAEADTFIRAMPHGYDS
                     MVGERGQKLSGGQRQRLTLARAILRDPAVLLLDEATSAIDNETEAAIQESLAQVSLDR
                     TTIVIAHRLSTIRHAHRIHVMEAGRIVEAGTHDELLDTGGLYAALWRVQTGELAPR"
     gene            5036..6922
                     /locus_tag="PLZ72_10705"
     CDS             5036..6922
                     /locus_tag="PLZ72_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNA36809.1"
                     /translation="MSTTVSTPDQTGVEPHELDRVVVRFAGDSGDGMQLVGDRFTELS
                     AIFGNDLATLPNYPAEIRAPAGTVAGVSSFQVHISDSDIVTPGDSPNVLVAMNPAALR
                     ANVADLAPGSTIIVNSDAFDERDLDKAGYAENPLLDGSLARFTLHQVPMTSITLEATA
                     ELGVKRRDAERSKNFFALGLICWMYTRPIQPLLDWITNKYDARPLVRDSNLAALRAGR
                     NFGETAELFDHPYSVAPAPLPPGTYSNVNGNTAVAWGLVAAGQLSGLRVVYASYPITP
                     ASDILHELSRRKNFDVWTMQAEDEIAAAAIAVGAAFAGTLAVTGTSGPGLDLKSEAIG
                     LAISLELPMVIVDVQRAGPSTGMPTKTEQTDLLSAIHGRHGESPLPVVAAKSPAHCFD
                     AAIEASRLALKYRTPVILLTDGYLANGAEPWRLPEMADLPDLSVEFATRTNHTEPDGR
                     ETFWPYLRDPETLTRPWAVPGTPGLQHRIGGIEKQDGSGNISYQPDNHEHMVRLRQAR
                     VDDIAADIPGVMLDEDDDAELLVIGWGSTWGAITAGVRRVRRQGRRVSQAHLVHVNPL
                     PPDLGEVLARYPKVLCPELNLGQMTGILRDRYLADVRSFTKVSGLPFGAHEMEERMIA
                     ELDR"
     gene            6919..7974
                     /locus_tag="PLZ72_10710"
     CDS             6919..7974
                     /locus_tag="PLZ72_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNA36810.1"
                     /translation="MSTTTDSTTNGSGSVTPVKLTRKDFMSDQEMRWCPGCGDYGITA
                     ALQLLLPDLGVQPHELVFISGIGCAARLPYYMDTYGIHSIHGRAPAIATGVALTRPDL
                     SVWVIGGDGDMLSIGANHLIHALRRNVDLKILMFNNQIYGLTKGQYSPTSEVGKVTKS
                     TPFGSIDQPFNPVSVALGAEASFVARTHEADRAHMQDVFRRAYEHEGAAFIEVYQNCN
                     VFNDGAFDDIMAKDNRDDMMIPLRHGEPVRFGAEGQHGVFQNEYAELFIGEVAEHGEG
                     ALVVHDEHRPDPTLAFALSRLAFGPHSPTPIGIFRDVDRPLYEAEVQEQVEEAIAQQG
                     PGDLAELIRSGPTWTVT"
     gene            complement(8008..8208)
                     /locus_tag="PLZ72_10715"
     CDS             complement(8008..8208)
                     /locus_tag="PLZ72_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36811.1"
                     /translation="MSDETHEHHFQETKDGKANIDPKPLGKPESISQVLHDVAEGAAE
                     FLEGKPPKGAKDSGHVEGADRE"
     gene            8346..8813
                     /locus_tag="PLZ72_10720"
     CDS             8346..8813
                     /locus_tag="PLZ72_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36812.1"
                     /translation="MGGTSFAAAQVAGVATVVAVLAAAAATAVVAFVAVELRRATPPD
                     QPDTHPWISGSPAEATVVSVELGDGVRNHVTVRCVVEFAVVTAEDRNPFPFVVDCLLP
                     AEVVPSAGDCWSARVNATSLGPVVVVAPPDGGSRDSIGAEIAAVERDRRHDAA"
     assembly_gap    8932..9031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9083..9265
                     /locus_tag="PLZ72_10725"
     CDS             9083..9265
                     /locus_tag="PLZ72_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNA36813.1"
                     /translation="MACSCHGIPDGEIVDVIRQGACSVDEVTDVCRAGGSCGSCVGTI
                     ELLLTLERALDAEPAA"
     gene            9379..9846
                     /gene="bfr"
                     /locus_tag="PLZ72_10730"
     CDS             9379..9846
                     /gene="bfr"
                     /locus_tag="PLZ72_10730"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNA36814.1"
                     /translation="MQGNPEIIELLNDVITAELTAINQYFVHAKMCDNWGYKRLAEHI
                     RAESIDEMRHAEALVERVLYFDGFPNLQRLNPLRVGESVEEQFRNDLDLEYVAVERLN
                     NGIAAAVAAGDNGTRHLLEEILVSEEEHIDWLETQLTAIEQVGLQQYLAEQLG"
     gene            9885..10655
                     /locus_tag="PLZ72_10735"
     CDS             9885..10655
                     /locus_tag="PLZ72_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36815.1"
                     /translation="MEISGSRVLLTGASGGLGAAIARDLSRRGADLVLTARRRELLDA
                     LAAETRGRVVVADLAVRADVETLIEESRGVDVLVLNAGVGADVGIRSETAEHVDTTID
                     VNLRAPIVMAVAFAQHKLSVGEPGQIVLVGSLSGLAATPDTRMYNATKFGLRGFAHSL
                     RQDLDGSGIGLTLVSPGFIRDAGMFAEGGIDLPPGVRTKSPADVAAGVVRAIESNPAE
                     VFVSPVELRLAATLATVAPAFSAAVQKRAGTAQMKSGS"
BASE COUNT         1498 a         3858 c         3625 g         1554 t
ORIGIN      
        1 gatggctcga tcggcggcag gacacacggg ggcggagaag cgggcgggtt ggagcttcct
       61 ggccgtgtcg gtcaacaact cgctctacac cctcaacgcc ttccggccga gcaaccggtt
      121 ccggctcctg ttcggctgga gcttcttcgc cagctgggtc accatcgagc tggcgcccta
      181 catcctggcc gtcgaggtgc tgctcggcgc ctggttcgtc aagcgcgggg cgctgcgcac
      241 cgcgccgggc cgggccggga tggtcctcac cctggggacc tgcgtcggca tggcggcgac
      301 catccgccag agcttccggg cccgccacga gatccgccag gccctgcgcg acgtcgtcga
      361 ggtcgaccgg gccgatccgc tgccggtgcg ctgggagcgg cgggtcgagt tcgcccgggc
      421 cggcggccgg tccctgcgca tggacatcct gcgtccagac gtcgccgaca gcgccgatgt
      481 cgccgacggt ggcctcccgg gtcggcccgt cctggtccag gtccacggcg gtggctgggt
      541 gctcgggttc aaggagcgcc aggggcagct gctcatgcgt gagatggccg cccgcgggtg
      601 ggtgtgctgc aacgtcgact accggctcag ccccaccgcg accttccccg accacctcgt
      661 cgacgtgaag cgggccatcg cctggatccg tgacaacgcc gacgagctcg gcgccgaccc
      721 cggcttcgtc gccgtcaccg gcggctcggc cggcgggcac ctcaccgcgt tgacggcgct
      781 gaccgcgaac caggagcgct accagcccgg cttcgaaggc gccgacacct cggtcgtcgc
      841 ggccgtcccg ttctacggcg tctacgactt caccgaccgc aacggcgtgt ggccccccga
      901 cacccagtcg atgttcctcg ccccggtcgt catgaagtgc gaccccgacg aacgcccgga
      961 cctgtgggcc gacgcgtcgc cgatcgacca ggtgcacgcg ggcgcgccga cgttcatggt
     1021 catccacggc gaccgggacg tgctcgctcc ggtcgaggac gcccggacct tcgtggccgc
     1081 gctgcgcgac gtgtccgagc agcccgtcta ctacctggag ctcaagggcg cccagcacgc
     1141 gttcgagacg ttcgcgtcgg tgcgggccaa cgcggtgatc gccgcggtcg gccggttcct
     1201 cgacggggtc caccacgcgt acgtcaccgg accgggttgg gtggccgagc ccgacgaggt
     1261 gcgcgaggcg gtcgaggagc gcctcggcga tcgcatcacc gacccccagc cgatctcggg
     1321 gtcctgagcg acacaccttg tcgctcagga cccccagaat ccggcgacgt ccggctcaga
     1381 ggtaggcgtc gcccaggtgg gggcgcacga gggtgccctc gagcgacccg ccgggcaccc
     1441 ggtgcacctc gtgcagctcg gctcccggga cgatccgtgc cgcgacgtcg aggtcgtcgt
     1501 gacggccgac gacgccgctg tcgggcggag ccggccacga ctcggccacc ccgacgaggg
     1561 cggtcagcga cgcccagccg cgctggagcg cgccctggtc ggtgtcctcg ggcggctcgt
     1621 agccggtcgg ggtcagctcc cactgcaggg actggtcgtc gcgggacgac accgtggccc
     1681 ggaccgctcc gaacggggcc aggtgcgtcc agcggacacc cttcacctgg tcgcggtgca
     1741 gcgccgggac gttcaggttc aacacgctgc cgaccgggga gccgaccacc agcggcagcg
     1801 tctccaccgc gatgctcgcc gcggtgtccc agcgccagcc ctcggctccg ggaccgccgc
     1861 cggccgcggc gtcgacgctg accgccaggc cggacacccc gaagttctgt cctgccaacg
     1921 cggcgccgac cgtgcccgag tgcaggatcg cccgaccggt gttggcgccg gcgttgatgc
     1981 ccgacaccac caggtccggc aactccccga acgccccgag tcggccggcg acgacgcaca
     2041 gcgccggtgg tccgtcgaag gcccacgcct cgattccgtc gccatcgggg atccgggcac
     2101 tcgccacgcc cagggcgctg tcgggacgga ggttccccaa cgcagcaccc gccccgctcc
     2161 agtcgctcga cggtgccacc accacgatct cgtggccggt gccggccagc gcccgggcca
     2221 gctcgtggat gccttcgctg tcgatgccgt cgtcgttggt gaccaggatc ctcatcnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgt cgcggatccg gcagggcggg
     2401 aggtcctgtc ctggctcgtg gtcgcctcgc tcctcgaggc gcacgccgag ttgggcgtgg
     2461 tcgagacgca ccccggctac gacggcgagt acgactgcct gtcggtgtac gaccggaccc
     2521 gcctcgaccg gggcccgctg ctcgacctcg accggaacgg cgtcgcccac atcgccccgc
     2581 tcgccggcgg cgccgacacg tggcccgacg tctgggaccg ctgcgcggag gacggaccgg
     2641 tggtggtggc cgggggactc cgggcgcggg tgggactcgg tgcgctgtgc ccccacggcg
     2701 gccgcgagtc gcacaccgtg tcccagatcg cccggcggct gctcaagctg cacctctcgg
     2761 agatcccggg cacgtggagc tgccgcaacg gcatcgccga cagctcacgg ggacccgaac
     2821 gccgcctcgg gctcttcgcc gaggtccccg ccgggatgga gcggctggcc gcggcgccca
     2881 ctcacccgtt gggggatccg gcctacgcgt tctggttcct gctgcgcgac ggcgtgccga
     2941 tcagctgcct cgacacgttc cactgatcct ccccgacgca cacccggttg ggggcggcgc
     3001 tccgaaaccg tctggggcga ccgtccggcc ggcagcagaa tgacccggtg agcaccacct
     3061 cgttcccgct gtcgaccacg cccccgttgc gggacctgtg gcgccacgcc gcccgacgcc
     3121 gaccgacggt ggtggcggcg acggtcctgt cgacggccaa caagctcttc gacgtgctgc
     3181 ccgagctgct catcggcgtc gcggtcgacg tggtggtcaa cgagggcgag tcgatggtcg
     3241 gtcgcctcac cggagtgcag gaccgcttcg gccagctcgt gatcctggcg atcgtcaccg
     3301 tcgtgatctg ggtgtgcgag tccgcgaccg actacgccgc gcaccgcacg tggcggaacc
     3361 tggcccaggc cgtcgagcac gacacccgca tggaggcgta ccgccacatc cagtccctcg
     3421 agatggcgta cttcgaggac accacctccg gcgggctgat gacgatcctc aacgacgacg
     3481 tcaaccagct cgaacggttc ctcgacgtgg gagcggaccg catcatccag tcgatcgcca
     3541 acgtcgtgct cgtcggcgcg gtcttcgcgg tgatctcccc gacgctgatg gtgctggcgt
     3601 tcctccccat cccggtgatc gtggtcggct ccctgcgcta ccagaagcgc ctcgaacccc
     3661 gctacgacgc ggtgcgggcc gccgcggccg acatcggcca caccctgtcc aacagcctgg
     3721 gcggggtggc gacggtcaag gcgttcagcg gcgaggagcg cgaggtggcg cgtctcgagg
     3781 ccgagagctc caactaccgc gagaccaacg ggcgggcgat cgccctgtcg agcgccttca
     3841 tcccgttgat ccgcatggcg atcctggccg ggttcaccat gacgctgctc gtcggtggca
     3901 aggccgccct cgacgggaac ctggccgtcg gcatgtactc ggtgctggtg ttcatgaccc
     3961 aacggctcct gtggccgttg accgagctgg gcgagatcct cgacctctac cagcgggcga
     4021 tggcgagctg ccgccggatc ttcgggctgc tggagatgga accccggatc cggcccggat
     4081 cccgggacct tcccgttccg gtgcgtggcg acgtgcgctt cgacgacgtc accttcagct
     4141 accaccgcgc cgcgcccgtg ccggccggga ccgccccgga cgaggaggcg ccgggaccgc
     4201 ctccgggcag cgacgacgcc gaccgcaacg tcctgcgggg gttctcgctg cacgtgcccg
     4261 cgggggagac ccacgccatc gtcggttcga ccggctcggg caagtcgacg gtgctcaagc
     4321 tcctcctgcg gctctacgaa ccggactcgg gcacggtcac cctggacgac atcccggtcg
     4381 ccgagctcac cttcgcctcg ttgcggggca gcaccggtct ggtcccccag gaggtgttcc
     4441 tgttccacgg gagcgtcgcg gacaacatcg cctacggccg tcccgacgcg acgcgtgatc
     4501 agatcgtcgc cgcggcggag ctggccgagg ccgacacgtt catccgggcg atgccccacg
     4561 gctacgactc gatggtgggc gagcgcggcc agaagctgtc gggtggtcag cgccagcgcc
     4621 tcaccctggc ccgggcgatc ctgcgcgacc cggcggtgct cctgctcgac gaggccacct
     4681 cggccatcga caacgagacc gaggccgcca tccaggagtc gctggcgcag gtgtcgctcg
     4741 accggacgac gatcgtgatc gcccaccgcc tgtcgaccat ccgccacgcc caccggatcc
     4801 acgtcatgga ggcggggcgg atcgtcgagg cgggaaccca cgacgagctg ctcgacacgg
     4861 gcggcttgta cgccgcgctg tggcgggtcc agaccggcga gctcgccccc cgctgatccg
     4921 ggccgcagcc gccctatcgg agcgccccgc ggggaaatct cggcgatggg atcgcggtgg
     4981 gtgagccggg cggtaggttc gaggggcgaa ggaccacgcg gaacaagggg gttccatgtc
     5041 caccacagtc agcacgccgg accagaccgg ggtcgagccc cacgagctcg atcgggtggt
     5101 cgtccgattc gccggtgact ccggcgacgg gatgcagctg gtcggcgacc gcttcaccga
     5161 gctgtcggcc atcttcggca acgacctggc cacccttccg aactacccgg ccgagatccg
     5221 cgcccccgcc ggcaccgtgg cgggggtgtc gtcgttccag gtccacatct ccgacagcga
     5281 catcgtcact ccgggggact cgcccaacgt gctcgtcgcc atgaacccgg ccgctctgcg
     5341 ggccaacgtc gcggacctgg cgcccggctc gacgatcatc gtcaactccg acgccttcga
     5401 cgagcgcgac ctcgacaagg ccggctacgc cgagaacccg ctcctcgacg ggtccctggc
     5461 ccgcttcacg ctgcaccagg tgccgatgac gtcgatcacc ctcgaggcca ccgccgagct
     5521 gggcgtcaag cgccgcgacg ccgagcggtc caagaacttc ttcgccctcg ggctgatctg
     5581 ctggatgtac acccgaccga tccagccgct gctcgactgg atcaccaaca agtacgacgc
     5641 ccgaccgctg gtgcgcgact ccaacctggc ggcgctgcgg gccggacgga acttcggcga
     5701 gaccgccgag ctgttcgacc acccgtactc ggtcgcgccg gccccgctgc cgccgggcac
     5761 ctactccaac gtcaacggca acaccgcggt cgcctggggt ctggtggcgg ccggccagct
     5821 ctcgggcctg cgggtcgtgt acgccagcta cccgatcacc ccggcgtccg acatcctcca
     5881 cgagctcagc cggcgcaaga acttcgacgt gtggaccatg caggccgagg acgagatcgc
     5941 ggccgcggcc atcgcggtgg gcgccgcgtt cgccggcacc ctcgccgtga ccggcacctc
     6001 gggcccgggc ctcgacctca agtccgaggc gatcggactg gcgatcagcc tcgagctgcc
     6061 gatggtcatc gtcgacgtgc agcgggccgg gccgtcgacc ggcatgccga ccaagaccga
     6121 gcagaccgac ctgctgtcgg ccatccacgg ccgtcacggc gagtcgccgc ttccggtggt
     6181 ggcggccaag tcgcccgcgc actgcttcga cgccgccatc gaggcctccc gactggcgct
     6241 gaagtaccgc accccggtca tcctgctgac cgacggctac ctggccaacg gtgccgaacc
     6301 gtggcggctg ccggagatgg ccgacctgcc cgacctgtcg gtggagttcg ccacccggac
     6361 caaccacacc gaacccgacg gccgtgagac cttctggccg tacctgcggg accccgagac
     6421 cctcacccgg ccgtgggccg tccccggcac ccccgggctg cagcaccgca tcggcgggat
     6481 cgagaagcag gacggctcgg gcaacatcag ctaccagccc gacaaccacg agcacatggt
     6541 ccgacttcgc caggctcgcg tcgacgacat cgcggccgac atcccgggcg tgatgctcga
     6601 cgaggacgac gacgccgagc tgctggtcat cggctggggt tcgacctggg gggcgatcac
     6661 cgccggggtc cgtcgggtcc gccgtcaggg tcgacgggtc tcccaggcgc acctcgtgca
     6721 cgtgaacccg ctgccgccgg acctggggga agtgctggcg cggtacccca aggtcctgtg
     6781 ccccgagctc aacctcggtc agatgaccgg catcctgcgg gaccgctacc tggccgacgt
     6841 gaggagcttc accaaggtgt cggggctgcc cttcggcgcc catgagatgg aagagcggat
     6901 gatcgcggag ctggaccgat gagcacgacg accgacagca ccaccaacgg ctccgggagc
     6961 gtcaccccgg tcaagctgac ccgcaaggac ttcatgtccg accaggagat gcgctggtgt
     7021 ccgggttgtg gcgactacgg catcaccgcc gcgctgcagc tgcttctgcc cgacctcggg
     7081 gtgcagcccc acgagctcgt cttcatctcc gggatcggct gcgcagcccg cctgccgtac
     7141 tacatggaca cctacggcat ccactcgatc cacggtcggg cgccggcgat cgccacgggc
     7201 gtggccctga cccgacccga cctgtcggtg tgggtgatcg gcggcgacgg cgacatgctc
     7261 tcgatcggcg ccaaccacct gatccacgcc ctgcgccgca acgtcgacct caagatcctg
     7321 atgttcaaca accagatcta cggtctgacc aagggccagt actcaccgac ctccgaggtc
     7381 ggcaaggtca ccaagtcgac gccgttcggt tcgatcgacc agccgttcaa cccggtgtcg
     7441 gtggcgctgg gggccgaggc ctcgttcgtg gcccgcaccc acgaagcgga ccgtgcccac
     7501 atgcaggacg tgttccgtcg ggcctacgag cacgagggtg ccgcgttcat cgaggtgtac
     7561 cagaactgca acgtcttcaa cgacggcgcc ttcgacgaca tcatggccaa ggacaaccgt
     7621 gacgacatga tgatcccgtt gcgccacggc gagccggtcc gcttcggggc cgagggccag
     7681 cacggcgtgt tccagaacga gtacgccgag ctgttcatcg gcgaggtcgc cgagcacggg
     7741 gagggcgcac tcgtcgtcca cgacgagcac cgccccgacc cgacgctcgc gttcgccctg
     7801 tcgcggctgg cgttcgggcc gcactcgccg acgcccatcg gcatcttccg cgacgtggac
     7861 cgacccctct acgaggccga ggtgcaggag caggtcgagg aggcgatcgc gcagcagggt
     7921 ccgggcgacc tggccgagct catccgttcc ggccccacct ggaccgtcac ctgagcagcg
     7981 gccgagggcg gcgatccgcc gtgggtgcta ctcgcggtcc gcgccctcga cgtggccgga
     8041 gtccttggcg cccttcggcg gcttgccctc gaggaactcg gcggcccctt cggcgacgtc
     8101 gtggagcacc tggctgatcg actcgggctt gccgaggggc ttcgggtcga tgttcgcctt
     8161 cccgtccttg gtctcctgga agtggtgctc gtgggtctcg tcgctcatcg gtgtctcccg
     8221 gtcgtcggtg ctccccatca tggcgcggct cgactccgtt ccgcgcgtca aggtggacat
     8281 cgctcggctc cggttgcgta gccgttactg agcccgtcgg gccgggttgc gaccagactc
     8341 gccccatggg cgggacctcg ttcgcagcgg cacaggtggc gggcgtcgcg accgtggtcg
     8401 cggtcctggc cgcggccgcg gcgacggcgg tcgtggcgtt cgtcgcggtc gagctgcgtc
     8461 gggcgacgcc gcccgatcaa cccgacaccc acccctggat ctccggttct cccgccgagg
     8521 ccaccgtggt gtcggtcgag ctgggcgacg gggtgcgcaa ccacgtcacc gtgcgctgcg
     8581 tcgtcgagtt cgcggtcgtc acggccgagg accggaaccc gttcccgttc gtggtcgact
     8641 gcctcctgcc cgccgaggtc gtcccgtccg cgggtgactg ctggtcggcc cgggtcaacg
     8701 cgacgtcact cggcccggtc gtcgtggtcg cgccgccgga cggtgggtcg agggactcga
     8761 tcggagccga gatcgctgct gtggagcggg atcggcgtca cgacgccgcc tgatcgatcc
     8821 gaatacggct ggtgccggag tcctgcagga agtcgtgcgg gagcccgact gtcccggatc
     8881 aggggttgcc atcctcgcag tgttaggtat tctgaacacc tatggcgatg gnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatcagggg ttgccatcct cgcagtgtta
     9061 ggtattctga acacctatgg cgatggcttg ctcctgccac gggatcccag acggcgagat
     9121 cgtcgacgtc atccgtcagg gcgcgtgctc ggtcgacgag gtcaccgacg tctgccgcgc
     9181 cggcgggagc tgcggatcct gcgtcggcac catcgagctg ctgctgacgc tcgagcgcgc
     9241 gctcgacgcc gagcccgcgg cctgagcccc tcccgatcga cccccgccct gccgcccgcg
     9301 ccgcgcctcc cacggcgccc gtcgggctcg tccacgacgg tgggtagagt gggccgcggc
     9361 catccgagga ggatcagcgt gcagggcaac ccggagatca tcgagcttct gaacgacgtc
     9421 atcaccgccg agctcacggc gatcaaccag tacttcgtgc acgccaagat gtgcgacaac
     9481 tggggctaca agcggctggc cgagcacatc cgggccgagt cgatcgacga gatgcgccac
     9541 gccgaggccc tggtcgagcg ggtcctgtac ttcgacggct tccccaacct ccagcgcctc
     9601 aacccgctgc gggtgggcga gtcggtcgag gagcagttcc gcaacgacct ggacctggag
     9661 tacgtggcgg tggagcggct gaacaacggc atcgccgccg cggtcgccgc cggcgacaac
     9721 ggcacccgtc acctgctcga ggagatcctc gtctccgaag aggagcacat cgactggctc
     9781 gagacgcagc tgaccgccat cgagcaggtc ggcctccagc agtacctggc cgaacagctc
     9841 ggctgacgtc gggccgtccc gagccggccg ctacggtcgg ctccatggag atctccggaa
     9901 gtcgggtcct gctcaccggc gccagcggtg gcctgggtgc cgccatcgca cgcgacctgt
     9961 cccgtcgggg tgccgatctc gtcctcaccg cccgccgccg tgagctgctc gacgccctgg
    10021 cggccgagac gcgtggccgg gtggtcgtcg ccgatctggc cgtccgggcc gacgtcgaga
    10081 ccctgatcga ggagtcccgc ggggtcgacg tgctcgtgct caacgccggg gtcggagcgg
    10141 acgtcgggat caggagcgag accgccgagc acgtcgacac cacgatcgac gtgaacctgc
    10201 gggccccgat cgtcatggcg gtcgccttcg cccagcacaa gctctcggtc ggtgagccgg
    10261 gtcagatcgt gctggtcgga tcgctgtcgg ggctggccgc cactcccgac acccgcatgt
    10321 acaacgccac caagttcggg ctgcggggct tcgcccactc gctgcgccag gacctggacg
    10381 gatcggggat cgggttgacg ctggtgtcgc cggggttcat ccgcgacgcc ggcatgttcg
    10441 ccgagggcgg catcgacctg ccgccgggcg tgcgcaccaa gtcgcccgcg gatgtggccg
    10501 ccggggtggt cagggcgatt gagtcgaacc cggccgaggt gttcgtgtcg ccggtcgagt
    10561 tgcgcctggc cgccacgctg gccacggtgg ccccggcgtt cagcgcggcg gtccagaagc
    10621 gtgccggcac cgcccagatg aagtccggca gctgagtcga ccggactcga ccggccgatc
    10681 agagcccgga cgcgccacgg ccccgggcgt gacgcccggg gccgtgggga ctacc
//
LOCUS       DAOQOD010000106        10521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2270_length_10521_cov_12.580547, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000106 DAOQOD010000000
VERSION     DAOQOD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_10521_cov_12.580547"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLZ72_10740"
     CDS             <1..577
                     /locus_tag="PLZ72_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA36816.1"
                     /translation="ADSVVVAAPAPTAAELVGDLSPAAAAHLRRIVHVPVAFVTVALD
                     ASRVTVPEGLSGVLVPRDAGLLATAVSFGSRKWPHWSDGAHTVLRVAVGHRHDDRPAD
                     LDDDELVAAVRADLSTVLGVGVAPVATRVSRWSPGFAQYQVGHLELVGRIRSALGADA
                     PAVRVVGADLGGLGLPACIAQGRGASRELLF"
     gene            complement(680..1075)
                     /locus_tag="PLZ72_10745"
     CDS             complement(680..1075)
                     /locus_tag="PLZ72_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNA36817.1"
                     /translation="MSASVGALVVAAATVVGCSGSDESTTQTSQLTGAAAQGQTAVRD
                     LGCTTCHNAEGRDGAGPTFKGLAGSQVQLADGTTVTADDDYLRVAITDPDRQVVDGYS
                     AIMPTRKLTDEQVEQIIAYLDAIGSTSGS"
     gene            1204..2811
                     /gene="lnt"
                     /locus_tag="PLZ72_10750"
     CDS             1204..2811
                     /gene="lnt"
                     /locus_tag="PLZ72_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNA36818.1"
                     /translation="MDGPVDSDQHPARVTARPAAEGDPTRPDPTRPLPLGLAALLATV
                     AGVCLCLALPPWGWWPLAPIGVALFVVALGGRTRRARAGLGWLVGVMWFGPSTLWMAG
                     LTLPGYVVGVLVAWGGMVAVVAALCPADRRRLVALPALLVVFEWFHQQAPFGGIPLSL
                     LATTQTDGPLLPLARLGGVLLVGLGVAGLGVALVLIGERRFRVAAIVAATVVAVTILG
                     AVWPIGSPVARVRVAAVQGGGPQGTRYSSDQAPIVFQRHLEATRTITGPVDVVVWPEN
                     VINLGRPFLDSPELRLMVEQAARLDAPIVAGVVESVGREHFVNYVVVVHPDGRVTDRY
                     DKERRVPFGEYVPLRWLFEPVAAGTLPARDQIPGTGTSVVRTGHGKMAVAISWEIFFG
                     RRVREGVRAGGQVVLNPTNGSSYWLTQVQTQQVAMSQLRAVESGRWVVQVAPTGFSAF
                     VDPDGGVHQRTEVSEQEVITRTIERHDATVPAQALGDLPALLLALGALLWTFAPSLPP
                     FRSARSRRSPGSRTTERSVGLRSRPGG"
     gene            complement(2789..3205)
                     /gene="dtd"
                     /locus_tag="PLZ72_10755"
     CDS             complement(2789..3205)
                     /gene="dtd"
                     /locus_tag="PLZ72_10755"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNA36819.1"
                     /translation="MTVTHPDGEQELVGAIGEGLCVLLGVTHDDDDRAARAMAAKLWN
                     LRVFDDADGVMNLSAAELGRPVLVVSQFTLYGDTRKGRRPSYVAAARPEVAEPLVERV
                     VAELRSLGATVATGRFRTDMAVELVNDGPVTLLVET"
     gene            3315..4655
                     /locus_tag="PLZ72_10760"
     CDS             3315..4655
                     /locus_tag="PLZ72_10760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA36820.1"
                     /translation="MRSAPSRAPSRPRTVPGPDDPLRIAFLTYRGKPHVGGQGVYTRH
                     LTKALVDMGHHAEVLSGQPFPIVDERVPLVELPSLDIYNDHFPMRMPGIWELKDRWDW
                     AEVTAFSSGTFPEPLAFSLRAWDHLRQRQGDFDLVQDNQCLGYGLLLMERMGLPVLGT
                     VHHPITVDRRLEMEHAESPRQRWSKARWYAFTKMQTRVAKRLRRVITVSRNSYEDICR
                     DHLVSPERLHIVPVGVDPELFAPMPGVERNPNQIISTASSDVAMKGQRYLLEALAKLR
                     TEFPDLKLIMVGRLKEGSAAQRTIETLGLDGAVEFVSGVPDETIVELYNSSACAVVPS
                     LYEGFSLPAIEAMSTGCPLVATTGGAIPEVSGPDGDTCIAVAPGDADALAAGIRRALT
                     DPDEAAAIGLRGRDRVIHRWTWRQTAEKTVVHYRALLEESARTAGAATTTRIGG"
     gene            4660..5493
                     /locus_tag="PLZ72_10765"
     CDS             4660..5493
                     /locus_tag="PLZ72_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA36821.1"
                     /translation="MLTVDFDLLGLQRGDLVLDMGAGAGRHSFECFRRGAQVVSLDYG
                     FDELPPVRDLFWAMQDSGEAPLGSLGACINGDALRLPFPDHTFDRIICSEVFEHIPDD
                     RGAMAELHRVLRPGGVLAATVPSWMPEKLCWQLSAEYHAPLAAGGHVRIYTEAMLKDR
                     LIDAGFLPESSHRAHALHSPYWWLKCLVGPTNDTHPLVQAYHKLLVWDIASAPALTRT
                     TERILNPVLGKSVVVYSRRTIGGGAADATAAATRTADDTVPTDPSVAPDAIEEAAGVT
                     V"
     gene            5480..6562
                     /locus_tag="PLZ72_10770"
     CDS             5480..6562
                     /locus_tag="PLZ72_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36822.1"
                     /translation="MSRSEVFLSDVSGIVTAAQLTATVDAVAEWQLESGMVPWFPGGH
                     ADTWNHTEALMALMLGGRRTEAELGFRWLQSMQRPDGSWHQYYLADRVEQDKLDANCC
                     AYVATGVWFHWLLFEDRGVLEDLWPMVSAAVEFVLDLQTRRGEILWARHADGTPWSFA
                     LLTGSSSISHSLRCAIAIAEVLGHDKPDWELSAARLSRVIRDEPDAFAPKHRWAMDWY
                     YPVLCGAITGESGRERLAHRYDAFITEDWGVRCVSDRPWITAAETCECAMAHLAVGET
                     DRATELFTWAQRLRTEEDRYWTGIVLPDQVHFPGGEQSTYTSSAIILAADALVGASPA
                     SRLFTRPDELPELIEDQTRSDVVEPS"
     gene            6543..7031
                     /locus_tag="PLZ72_10775"
     CDS             6543..7031
                     /locus_tag="PLZ72_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36823.1"
                     /translation="MSSNRPDHLPALRLRPAVALAAAALVAAATLLIAPASVGAQEGS
                     GSSGSATTVTTVVADVGPVSECSPGHIVRRPDCGIPPESPTDPGGWLQVSLFYLICAA
                     VVGLIAFVWWRSRVARNERRAAGLDPVDVARARGQGVRRSTRRDPVAETSSKVPDANA
                     GR"
     gene            complement(6950..7600)
                     /locus_tag="PLZ72_10780"
     CDS             complement(6950..7600)
                     /locus_tag="PLZ72_10780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA36824.1"
                     /translation="MDTAPEMDPSTRAAAEAARGFMPPDEGLALFRAGVEAAAAVPGA
                     PLLEVGSYCGKSSVYLGAAARTADTVLVAVDHHRGSEENQPGWEWHEPDLVDPAVGRM
                     DTLPLFRRTVFDAGLEPWVVAVVGDSPTVARIWSTPCALVFIDGGHGEEPADQDYRLW
                     TPKVAPGGLLAVHDVFPDPADGGRPPYERIYLPALASGTFEEVSATGSLRVLRRTP"
     gene            complement(7618..8127)
                     /locus_tag="PLZ72_10785"
     CDS             complement(7618..8127)
                     /locus_tag="PLZ72_10785"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNA36825.1"
                     /translation="MKISPSAVIDHARSEQGRKQIRYAAVSVVFVPVGQILIQVLGAM
                     VFDRDYTKASIVAAAILTVPNFFANKMWVWKDTSRDKLRTQVLVFWVAAMLGVAAATG
                     LTYLVEQQFHNEGAVEALAVFCAQLVGFGIVWVGRYLILDRWLFKVTHHGEEPGDDEL
                     DMLHGDLPI"
     gene            8222..9100
                     /gene="tesB"
                     /locus_tag="PLZ72_10790"
     CDS             8222..9100
                     /gene="tesB"
                     /locus_tag="PLZ72_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HNA36826.1"
                     /translation="MDQPAVDALIALLDLEPIEVNIFRGAVSPDRGDQRVFGGQVAGQ
                     ALVAGARTVEADHVVHSLHAQFLRPGDPAIPIIYEVDRSRDGHSFSTRRVRAVQHGRS
                     IFTLSASFQKPEDGFDHQFEMPPGLPDPEDLPDFKERMAPWKDAFADYYDRPRPIDTR
                     YCETGPWERREDLPPFQNVWMRANGVLPDDPVLHICVLTYASDMTLLDTSLQPHGSRF
                     TVGEVFMASLDHAMWFHRPFRSDEWLLYAQDTPSASGGRGLGRGLVYSRDGRLVASVV
                     QEGLIRPMRSHQEGDR"
     gene            9097..10395
                     /locus_tag="PLZ72_10795"
     CDS             9097..10395
                     /locus_tag="PLZ72_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNA36827.1"
                     /translation="MSSTPICTTSRRALAVLTAVVALAAGCSGDDTSGSTSTTKARDG
                     STSSTSTTADGDGTARAPLDATPVRLVSLDLDANDPIDLAYRSDSTSLLVAERGGTVR
                     EAQRAGDGFRFTDEPVIDLTAAVGSTDMERGLLGIAVAPDGEHLYVSYTEASHGDSRI
                     DEYGLSGEDGSLRADPDTRRQLVAIEQPFPNHNGGSLRFGPDGMLYAGFGDGGAADDP
                     EGHGQARNTLLGKILRIDPSAPDGIPADNPFVGGDADLSDAQPLIWATGLRNPWRIDF
                     DPANGDLWIGDVGQNRIEEIDRLVADGDRPAGYGANLGWDLFEGTERFESPDPAPGPA
                     SEGPFVTPILTYSHDEGGCSVTGGVVVRDPRLAGLDGAFLYSDFCAGGVRAVRPSTGD
                     SIDTADLGLEIGSVVSFARGPEGEVYVISMSDGVHRIDPA"
BASE COUNT         1429 a         3977 c         3608 g         1507 t
ORIGIN      
        1 cgccgactcg gtcgtggtcg cggcacccgc cccgacggcc gccgagctgg tcggcgatct
       61 cagccctgcc gccgcggcgc acctgcgccg catcgtccac gtgccggtcg cgttcgtgac
      121 cgtggccctc gacgcctccc gggtcaccgt gcccgagggt ctgtccggcg tgctcgtccc
      181 ccgcgacgcc gggttgctcg ccaccgcggt ctcgttcggt tcgaggaagt ggccgcactg
      241 gagcgacggg gcgcacaccg tgctgcgcgt ggcggtcgga caccgccacg acgaccgacc
      301 ggccgacctc gacgacgacg agttggtggc ggcggtgcgt gcggacctct ccacggtgct
      361 cggggtcggg gtcgcgccgg tggccacgcg ggtcagccgt tggtcaccgg gcttcgccca
      421 gtaccaggtc ggccacctcg aactggtcgg tcggatccgc tccgcgctgg gcgccgacgc
      481 ccccgcggtg cgggtggtgg gagcggacct cggcggcctc ggcctgcccg cctgcatcgc
      541 tcagggccgg ggcgcatcgc gggagctgct cttctgacca caacaacgca cgggcgagaa
      601 cgcgcgggcg agagggcgga gggacgagag aacgcacgac cgagccaccg gttcccccca
      661 ggccgggacc ggtgaccggt caggagccgg acgtcgaccc gatggcgtcg aggtaggcga
      721 tgatctgctc gacctgctcg tcggtgagct tccgcgtcgg catgatcgcc gagtagccgt
      781 cgaccacctg gcggtcgggg tcggtgatcg ccacccgcag gtagtcgtcg tccgcggtca
      841 ccgtcgtgcc gtcggcgagc tggacctgcg aaccggccag gcccttgaac gtcggacccg
      901 caccgtcacg gccctcggcg ttgtggcacg tcgtgcaccc caggtcacgc acggccgtct
      961 ggccctgggc cgcagcaccg gtcagctggc tggtctgcgt cgtcgactcg tcggagccgc
     1021 tgcagccgac gaccgttgcg gccgccacga ccagcgcccc gaccgaggcc gacacgttcc
     1081 accggtggcg ccgggccggg ctcgttccgc ggacgtggga ggctcgttcg gaccgtcgtc
     1141 gtcgcatctc ctcagtctgc cccgcaccgg cggcatcgac ggagcgtgga aggatgctcg
     1201 accgtggacg gccccgtcga cagcgatcag cacccggccc gcgtgaccgc gcgcccggcc
     1261 gccgagggcg acccgacccg tcccgacccg acccgacccc tcccgctggg cttggccgcg
     1321 cttctggcga ccgtcgccgg ggtgtgcctg tgcctggcgt tgccgccgtg ggggtggtgg
     1381 cccctcgccc cgatcggcgt cgcgctgttc gtcgtggcgc tcggcggtcg gacccgacgg
     1441 gcccgagccg gtctgggctg gctggtcggc gtcatgtggt tcggcccgtc cacgctgtgg
     1501 atggccgggc tgacccttcc cggctacgtc gtcggcgtcc tcgtcgcctg gggtggcatg
     1561 gtggcggtgg tcgcggcgct gtgcccggcg gaccgccgtc gactggtggc gctgcctgct
     1621 ctgctcgtcg tgttcgagtg gttccaccag caagcgccct tcggcgggat ccccctgtcc
     1681 ctgctggcga ccacccagac cgacggcccc cttctgccgc tcgcccggtt gggaggggtg
     1741 ctgctggtcg ggctcggggt ggccgggctc ggcgtggcac tggtactgat cggcgagcgc
     1801 cggttccggg tcgcggcgat cgtggccgcc acggtggtgg ccgtcacgat cctcggcgcc
     1861 gtgtggccga tcggatcacc cgtcgcccgg gtgcgcgtcg ccgcagtgca gggcggcggg
     1921 ccgcagggca cgcgctactc gagcgaccag gccccgatcg tgttccagcg ccacctcgag
     1981 gcgacccgca ccatcaccgg accggtcgac gtggtcgtct ggcccgagaa cgtgatcaac
     2041 ctgggccgac cgttcctcga cagccccgag ctgcgactga tggtggagca ggcggctcgg
     2101 ctcgacgccc ccatcgtcgc cggcgtggtc gagagcgtcg ggcgggagca cttcgtcaac
     2161 tacgtggtcg tcgtgcaccc ggacggtcgg gtcaccgacc gctacgacaa ggagcgccgg
     2221 gtcccgttcg gcgagtacgt cccgctgcgc tggctgttcg agccggtggc ggcgggcacc
     2281 ctccccgccc gggatcagat ccccggcacc ggcacctcgg tggtgcgcac cggccacggg
     2341 aagatggcgg tggcgatcag ctgggagatc ttcttcgggc gacgggtccg cgagggcgtc
     2401 cgggccggcg gccaggtggt gctcaacccg accaacgggt cgagctactg gttgacccag
     2461 gtccagaccc agcaggtcgc gatgtcgcag ctgcgcgctg tcgagtcggg gcggtgggtg
     2521 gtgcaggtcg ccccgaccgg gttcagcgcg ttcgtcgatc ccgacggcgg ggtgcaccaa
     2581 cggaccgagg tgagcgagca ggaggtcatc acccggacga tcgagcgcca cgacgccacc
     2641 gtgcccgccc aggccctggg cgacctcccg gcgctgctgc tggccctcgg cgccctcctg
     2701 tggaccttcg ccccctccct ccccccgttt cgtagtgcga gatccaggcg ttcgcctgga
     2761 tctcgcacta cagaacggtc ggtggggctc aggtctcgac caggagggtg accgggccgt
     2821 cgttgaccag ctcgacggcc atgtcggtgc ggaaccggcc ggtcgccacc gtcgccccca
     2881 gcgaccgcag ctcggccacc acccgctcca ccaggggctc ggcgacctcg ggtcgcgccg
     2941 cggccacgta gctcggccga cgacccttgc gggtgtcgcc gtagagggtg aactggctga
     3001 cgaccaggac cggtcgaccg agctcggcag ccgacaggtt catcacgccg tcggcgtcgt
     3061 cgaacacgcg caggttccac aacttggccg ccatcgccct cgcggcccga tcgtcgtcgt
     3121 cgtgggtgac gccgagaagg acgcacaggc cctctccgat cgccccgacg agctcctgtt
     3181 cgccgtccgg gtgggtcacg gtcaccgacg ccgagcgcac cctctggaca agaccacgca
     3241 ccgcgttcct ttccaccacc ctgcgtgaag ggcggcttga ccggtgggtc aatgcccggc
     3301 gaagctactc cgtcatgcga tccgctccgt cccgcgcccc gtcacgcccg cggacggttc
     3361 ccggacccga cgacccgctg cgcatcgcct tcctcaccta ccggggcaag ccgcacgtcg
     3421 gcggccaggg cgtctacacc cggcacctga ccaaggcgct ggtcgacatg ggtcaccacg
     3481 ccgaggtcct ctccggtcag cccttcccga tcgtcgacga gcgggtgccg ctggtcgagc
     3541 tgcccagcct cgacatctac aacgaccact tccccatgcg gatgcccggc atctgggagc
     3601 tgaaggaccg ctgggactgg gccgaggtga ccgccttctc gtccgggacg ttccccgagc
     3661 cgctcgcgtt ctcgctgcgg gcgtgggacc acctgcgcca gcgccagggc gacttcgacc
     3721 tggtgcagga caaccagtgc ctcggctacg ggctgctgct catggagcgg atgggcctgc
     3781 cggtcctcgg caccgtccac cacccgatca ccgtcgaccg ccgcttggag atggagcacg
     3841 ccgagtcgcc ccgtcagcgc tggtccaagg cccgctggta cgcgttcacc aagatgcaga
     3901 cccgggtggc caagcgcctg cgccgggtga tcaccgtgtc gcgcaactcc tacgaggaca
     3961 tctgccggga ccacctggtg tcgcccgagc ggctgcacat cgtgcccgtc ggcgtcgacc
     4021 ccgagctgtt cgcccccatg ccgggagtcg agcggaaccc gaaccagatc atctcgaccg
     4081 ccagctccga cgtggccatg aaggggcagc gctacctgct cgaggcgctg gccaagctgc
     4141 gcaccgagtt ccccgacctg aagctgatca tggtcggacg cctcaaggag ggctcggccg
     4201 cgcagcgcac gatcgagacc ctcggcctcg acggtgcggt cgagttcgtc tccggcgtcc
     4261 ccgacgagac gatcgtcgag ctctacaact cctctgcctg cgcggtggtc ccgtcgctct
     4321 acgagggctt ctcgctgccg gcgatcgagg cgatgagcac cggctgtccg ctggtggcca
     4381 ccaccggcgg cgccatcccc gaggtctccg gccccgacgg cgacacctgc atcgcggtcg
     4441 cgcccggcga cgccgatgcc ctggccgccg gcatccgccg ggcgctcacc gaccccgacg
     4501 aggccgccgc catcgggctg cgcggccgcg accgggtgat ccaccgctgg acctggcgcc
     4561 agaccgccga gaagaccgtc gtgcactacc gggccctgct cgaggagtcc gcccgcaccg
     4621 ccggcgccgc gaccaccacc cggatcggag gctgagccgg tgctgaccgt cgacttcgac
     4681 ctcctcggcc tgcagcgcgg cgacctggtg ctcgacatgg gcgcaggcgc cggccggcac
     4741 tccttcgagt gcttccgccg cggcgcgcag gtcgtgtcgc tcgactacgg cttcgacgag
     4801 ctgccgccgg tgcgggacct gttctgggcc atgcaggact ccggcgaggc cccgctcggg
     4861 tccctcggcg cctgcatcaa cggcgacgcc ctgcgcctgc cgttccctga ccacaccttc
     4921 gaccgcatca tctgctccga ggtcttcgag cacatccccg acgaccgcgg cgccatggcc
     4981 gagctgcacc gggtgcttcg ccccggcggt gtgctcgccg ccaccgtccc gtcgtggatg
     5041 cccgagaagc tctgctggca gctcagcgcc gagtaccacg ccccgctggc cgccggtggt
     5101 cacgtccgca tctacaccga ggcgatgctg aaggaccggc tgatcgacgc cgggttcctg
     5161 cccgagtcct cgcaccgggc ccacgccctg cactcgccgt actggtggct caagtgcctg
     5221 gtcggcccca ccaacgacac ccacccgctg gtgcaggcgt accacaagct cctcgtctgg
     5281 gacatcgcgt cggcgccggc gttgacccgc accaccgagc ggatcctcaa cccggtgctc
     5341 ggcaagagcg tcgtggtcta ctcccgccgc acgatcggcg gcggcgcagc cgacgccacc
     5401 gcggcggcga cccgcaccgc cgacgacacc gtccccacgg atccgtccgt tgcccccgac
     5461 gccatcgagg aagccgccgg tgtcacggtc tgaggtgttc ctgtccgacg tctccggcat
     5521 cgtcaccgcc gcgcagctga ccgccacggt cgacgcggtg gccgagtggc agctcgagtc
     5581 gggcatggtc ccgtggttcc ccggcggcca cgccgacacg tggaaccaca ccgaagccct
     5641 gatggcgctc atgctcggcg gccgccggac cgaagccgag ctcgggttcc ggtggctgca
     5701 gtccatgcaa cgacccgacg gctcctggca ccagtactac ctggcggatc gggtcgagca
     5761 ggacaagctc gacgccaact gctgcgccta cgtcgccacc ggcgtctggt tccactggct
     5821 gctgttcgag gaccgcggcg tgctcgagga cctgtggccg atggtgtcgg cggccgtcga
     5881 gttcgtcctc gacctgcaga cccggcgcgg cgagatcctc tgggcccgcc acgcggacgg
     5941 caccccgtgg agcttcgccc tcctcaccgg gtcgtcgtcg atcagccaca gcctgcgctg
     6001 cgccatcgcc atcgccgagg tcctcggcca cgacaagccc gactgggagc tgtccgcagc
     6061 gcgcctgtca cgggtcatcc gcgacgagcc cgacgcgttc gcccccaagc accggtgggc
     6121 catggactgg tactacccgg tgctctgcgg tgccatcacc ggcgagtccg gccgcgagcg
     6181 cctcgcccac cgctacgacg ccttcatcac cgaggactgg ggcgtccgct gcgtctcgga
     6241 ccgaccgtgg atcaccgcgg ccgagacgtg cgagtgcgcc atggcgcatc tggcggtcgg
     6301 agagaccgac cgggccacgg agctgttcac ctgggcacag cgtctgcgca ccgaggagga
     6361 ccgctactgg accggcatcg tgctgcccga ccaggtccac ttccccggcg gcgagcagtc
     6421 gacctacacc tcgtccgcga tcatcctggc cgcggacgcg ctggtcgggg cctcgccggc
     6481 cagccgcctg ttcacccgac ccgacgagct tcccgagctc atcgaggacc agactcgtag
     6541 cgatgtcgtc gaaccgtcct gaccacctcc cggctctccg cctccggccc gccgtcgcgt
     6601 tggctgcggc cgcactcgtc gccgcggcca cgttgctgat cgcccccgcc tcggtcggcg
     6661 cccaggaggg atcggggtcg tcgggttcgg cgaccacggt caccaccgtc gtcgccgacg
     6721 tggggcccgt gtcggagtgc tcccccggcc acatcgtccg tcgacccgac tgcggcatcc
     6781 cgcccgagtc ccccaccgac cccggcggct ggctgcaggt gtcgctcttc tacctgatct
     6841 gcgcggcggt cgtcggactg atcgccttcg tgtggtggcg gtcccgggtg gcgcgcaacg
     6901 aacgtcgggc cgccggcctc gaccccgtcg acgtcgcccg cgcccgcggt cagggcgtgc
     6961 ggcgcagcac ccgcagggac ccggtcgcgg agacctcctc gaaggtcccc gacgccaacg
     7021 ccggcaggta gatccgctcg tacgggggcc ggccgccgtc ggccgggtcg gggaacacgt
     7081 cgtggacggc cagcaggccg cccggcgcca ccttgggggt ccacagccgg tagtcctggt
     7141 ccgcgggctc ctcgccgtga ccgccgtcga tgaacaccag cgcgcacggc gtggaccaga
     7201 tccgggccac ggtgggcgag tcgccgacca ccgccaccac ccacggctcc agtccggcgt
     7261 cgaacaccgt gcgccggaac agcggcagcg tgtccatgcg gccgaccgct gggtcgacca
     7321 ggtcgggctc gtgccactcc cagcccggct ggttctcctc ggacccccgg tggtggtcga
     7381 ccgccaccaa cacggtgtcg gccgttcggg cggccgcccc caggtagacc gacgacttgc
     7441 cgcaatacga accgacctcg agcaggggcg ctccgggaac cgccgcggcg gcctcgactc
     7501 cggcccggaa cagggccagg ccctcgtcgg ggggcatgaa gccgcgggcg gcctcggccg
     7561 cggcacgggt gctcgggtcc atctcggggg cggtgtccac ggctcgggtg ggccggatca
     7621 gatcggcagg tcgccgtgga gcatgtcgag ctcgtcgtcg ccgggctcct caccgtggtg
     7681 ggtgaccttg aacagccagc gatccaggat caggtagcgg ccgacccaca cgatgccgaa
     7741 gccgacgagc tgggcgcaga acaccgcgag ggcctcgacg gcaccctcgt tgtggaactg
     7801 ctgctcgacc aggtaggtca gcccggtcgc ggcggccacg ccgagcatcg cagccaccca
     7861 gaacaccagc acctgggtcc ggagcttgtc gcgggaggtg tccttccaga cccacatctt
     7921 gttggcgaag aagttgggga cggtcaggat ggccgccgcg acgatcgacg ccttggtgta
     7981 gtcccggtcg aagaccatcg cgccgagcac ctggatcagg atctggccga cggggacgaa
     8041 caccaccgac accgcggcgt agcggatctg cttgcgcccc tgctcggaac gggcgtggtc
     8101 gatgaccgcg gaggggctga tcttcacggc tcaccaacct accggagcac tcctgcccct
     8161 ggcctcatcg gctcggaccg tggggaccat cggggccgtc gggacgggcc agactggagc
     8221 gatggaccag ccggccgtgg acgccctcat cgccctgctc gacctcgagc cgatcgaggt
     8281 gaacatcttc cggggcgccg tcagccccga ccggggcgac cagcgggtct tcggcggcca
     8341 ggtcgccggt caggccctgg tggccggcgc ccgcaccgtc gaggccgacc acgtcgtgca
     8401 ctccctgcac gcccagttcc tgcgcccggg cgatccggcc ataccgatca tctacgaggt
     8461 cgaccgctcc cgcgacggcc actcgttctc gacccggcgg gtgcgggcgg tgcagcacgg
     8521 gcggtcgatc ttcacgttgt cggccagctt ccagaagccc gaggacggct tcgaccacca
     8581 gttcgagatg cccccgggcc tgcccgaccc cgaggacctg cccgacttca aggagcggat
     8641 ggcgccgtgg aaggacgcct tcgccgacta ctacgaccgg ccccgaccca tcgacacccg
     8701 ctactgcgag accgggccgt gggagcgccg cgaggacctc cccccgttcc agaacgtgtg
     8761 gatgcgtgcc aacggcgtgc tccccgacga cccggtgctg cacatctgcg tgctgaccta
     8821 cgcgtcggac atgacgctcc tcgacacgtc gctgcaaccg cacgggtcgc gcttcaccgt
     8881 cggcgaggtg ttcatggcat ctctcgacca cgcgatgtgg ttccaccggc cgttccgctc
     8941 cgacgagtgg ctgctctacg cccaggacac acccagcgcc agcggcggcc ggggcctggg
     9001 ccgtggcctg gtgtacagcc gcgacggccg cctggtcgcc tcggtggtcc aggaaggact
     9061 catccgaccc atgcgttccc accaggaggg cgacaggtga gctcaacccc gatctgcacg
     9121 acatcgcgcc gggccctcgc ggtcctcacc gccgtggtgg ccctggccgc cggatgctcg
     9181 ggcgacgaca cctccgggtc gacctcgacg acgaaggccc gggacggctc gaccagcagc
     9241 accagcacta ccgccgacgg cgacggcacg gcccgggcgc cgctggacgc gacaccggtt
     9301 cggttggtgt cgctggacct cgacgcgaac gacccgatcg acctggcgta ccggtccgac
     9361 agcacatcgc tgctggtcgc cgagcggggc ggcacggtcc gcgaggcgca acgcgccgga
     9421 gacggcttcc gcttcaccga cgagccggtg atcgacctga ccgccgcggt cggcagcacc
     9481 gacatggagc gcgggctgct gggcatcgcg gtggcgcccg acggcgagca cctctacgtc
     9541 tcctacaccg aggcgtccca cggtgactcc cggatcgacg agtacgggtt gagcggtgag
     9601 gacggctccc tccgagccga ccccgacacc cgtcgccagc tcgtcgcgat cgagcagccg
     9661 ttcccgaacc acaacggcgg gtcgctccgg ttcggtcccg acgggatgct gtacgcaggc
     9721 ttcggagacg gaggcgcggc ggacgacccc gagggccacg gccaagcccg caacacgctg
     9781 ctgggcaaga tcctgcgcat cgatccgtcg gctcccgacg gcatccccgc cgacaacccg
     9841 ttcgtcggcg gggatgccga cctgtccgac gcccaaccgc tgatctgggc gaccgggctg
     9901 cgcaacccgt ggcgcatcga cttcgacccg gccaacggag acctgtggat cggcgacgtc
     9961 ggccagaacc gcatcgagga gatcgacagg ctcgtcgccg acggcgaccg acccgcgggc
    10021 tacggcgcca acctgggttg ggacctcttc gagggcaccg agcggttcga gagccccgat
    10081 cccgcgcccg gcccggcgag cgaggggccg ttcgtcaccc cgatcctgac ctactcccac
    10141 gacgagggcg gctgttcggt gaccggcggg gtcgtggtgc gcgacccgcg cctggccggg
    10201 ttggacgggg cgttcctcta cagcgacttc tgcgccggtg gggtgcgggc ggtgcggccg
    10261 tcgaccggcg actcgatcga caccgccgac cttggcctcg agatcggctc ggtcgtgtcg
    10321 ttcgcccggg gccccgaggg cgaggtgtac gtgatcagca tgtccgacgg ggtgcaccgc
    10381 atcgacccgg catgaccgac ccgcccccgc cgaacttgta gcccacacgc caccacagcg
    10441 accgccagct acaacatcga cggcgcccgc cgaacttgta gcccacacgc caccacagcg
    10501 accgccagct acaacatcgg c
//
LOCUS       DAOQOD010000107        10385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2316_length_10385_cov_11.001936, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000107 DAOQOD010000000
VERSION     DAOQOD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2316_length_10385_cov_11.001936"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLZ72_10800"
     CDS             <1..574
                     /locus_tag="PLZ72_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA36828.1"
                     /translation="GAVVPALPRRRRARRADPRAVDRSALDRQLGDLIDEHSDAVFRL
                     AVGILHDRQLAEDVVQETMMRAWTSMAGFRGDSSERTWVLRIAHNTAIDALRRRRDQA
                     TAPHDLPESTDRSDPADRATGRDRLAAVGRALAGLDETSRAVVVLREVEGMSYQEIAD
                     ALDVPVPTVKTRLLRARRALQAVDRDEEDT"
     gene            571..906
                     /locus_tag="PLZ72_10805"
     CDS             571..906
                     /locus_tag="PLZ72_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36829.1"
                     /translation="MSTHRDPDDVEPPDTHSDAELDRELARGDELLGASLRSLLSPPS
                     DIEERVADQVSQQLIGRSAAGAALDLLGLGVRTFMMFLSEDPSGADRDRERPDDGPNR
                     DEQDRRATA"
     gene            903..1628
                     /locus_tag="PLZ72_10810"
     CDS             903..1628
                     /locus_tag="PLZ72_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36830.1"
                     /translation="MNNGIWALVAVCSGLLLGELGGRLVRAARGGPDRPESVRSTARA
                     VGSLVFWSFTAVGLVIAVGALDIEALEDMGETISDSLPNLLLAAVMVIVGYAVAIAVA
                     AAVGQSARRASGVRQENLERVLRILITVIAVLLALHQAGVSDGMVAVLVVIVMAVPAV
                     AAALLSAFGGREVAAQLAAGRALRAQLRDGWTLRCETPDGEPVTGRIIRVHPTTVEIE
                     SADGGRGHVPHGYLLARPFTVSP"
     gene            1935..2237
                     /locus_tag="PLZ72_10815"
     CDS             1935..2237
                     /locus_tag="PLZ72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNA36831.1"
                     /translation="MVRHVVLLTFRPNTPRADVDAVADALRTLPGRIPELRGYDVGVD
                     LGLAGADGGNADLAVVADTDDVAGYEAYRDHPEHQRIITEMIRPILASRVAVQHER"
     gene            2545..3450
                     /locus_tag="PLZ72_10820"
     CDS             2545..3450
                     /locus_tag="PLZ72_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36832.1"
                     /translation="MTVKKLTGTDAFVITDLPDAETADGVVRCARKVLQDGARTMARS
                     RTYSWALLEQQISGASAGVNATDDRDAALRTFVDELSGEIGSGRLALDPGKGVTTEDL
                     AAWAELDRRSPEHTGRRDDLLAAGIVAAADVAVGGVAGASVVIEGAGASGSAIAAAFG
                     AADAEVVSTGDGPDVLRDAADVLVCGSRLGLVDHQVAAALPQRAVVPCGVAPVTAKGL
                     AVATGRGIVVVPDFLSLIGPLLGFRPDAGSSTESLLVDAAERVRTVLTDVIGHDEGAY
                     LGACYRAEAFLRTWQDTLPFGRPLA"
     gene            3487..4536
                     /locus_tag="PLZ72_10825"
     CDS             3487..4536
                     /locus_tag="PLZ72_10825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA36833.1"
                     /translation="MSTLEHPEPTVDLPAQPDGVPWPTGSWPEGPQLSGDPDRLAAIL
                     DPVFATEETPELARSLAFVAVQGGRVVAERYGPGVGPDSTLISWSMAKSVTHALVGIL
                     AGDGLLDPDAPAAVPEWSDPGDPRHPITVADLLAMRSGLAFVEDYLDDSVSDCLEMLW
                     GSGADDTAHYAASRPLEHPIGTVFNYSSGTTNILSRLIGSVAVRDVPSLLRERLLDPL
                     GMSSATAQCDASGTWVGSSYLFATARDFARFGLLYLRDGVWEGERLLPEGWVDSARTL
                     RSIDDENGWGYGHHWWIRVGDDRGTFWANGYEGQMICCVPALDLVVVRLGKTPSTLRP
                     HLERFFFDVVDCFAP"
     gene            4652..5428
                     /locus_tag="PLZ72_10830"
     CDS             4652..5428
                     /locus_tag="PLZ72_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNA36834.1"
                     /translation="MVDHRPSRPPRFTFHESDSLADERTHRKQRLAAAFRLFGRFGFG
                     EGIAGHITARDPEHADRFWVNPFGMNFRQIRASDLILVDHSGEVVEGDWPVNRAAFAI
                     HSRVHAARPDVVAAAHSHAVFGRSFSTLGRPLAPLTQDACAFHDDQAVFDDYTGVVED
                     PAEGDRIAAALGRRKLAILRNHGLLTVGTTVDAAAWWFITADRSCQAELLARAAGTPT
                     EIDPATAALTHSQVGSDAVGAFSFHPLYCWIVAEEPDLLE"
     gene            5513..6685
                     /locus_tag="PLZ72_10835"
     CDS             5513..6685
                     /locus_tag="PLZ72_10835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA36835.1"
                     /translation="MDWPLRCGVFMAPFHPNGQNPTLALERDLQLVEHLDRVGFHEAW
                     IGEHHSGGFEIIASPEVFIAAAAERTRHIDLGTGVVSLPYHNPLMTAQRIVQLDHMTR
                     GRVKFGVGPGALPSDAFMLGIDPADQRRRMEEALEVILHLLRSDEPITVETDWFTLRD
                     ARLHLRPYTHPHPEVAVAAMISPSGPRAAGRFGCSLLSIGATQRAGIDLLGQAWEVME
                     ERSAQFGNIADRRQWRLVTLMHLAETREQAVRDVEFGLADYARYFREVAALPIFPDAP
                     IETIVDTLKSSGAAVIGTPDDAIELIQHLIDESHGGFGTLLLQSHEWANPEATRRSYE
                     LFAQYVLPHFDPHVASTTASMEWVMANRPEQLGAAMNAIGAAIQQHAEEKAAAPPS"
     regulatory      6747..6859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      6860..6969
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            7008..9947
                     /gene="gcvP"
                     /locus_tag="PLZ72_10840"
     CDS             7008..9947
                     /gene="gcvP"
                     /locus_tag="PLZ72_10840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNA36836.1"
                     /translation="MSTDPTPEQPLNGTPTSGPGPDEGGFAERHLGPDADAVAHMLAT
                     LGRTDLDSLIDDAVPASIRQSRPLGLQAIADESEALEQLRRIAGTNTVVTSLIGCGYH
                     DTVTPPVILRNVLENPAWYTAYTPYQPEISQGRLEALLNFQTMVAELTGLDLANASLL
                     DEATAAAEAMTLARRRSKVAGDTFFVDSECHPQTIEVVETRAEPLGIKVVTGDPARDL
                     DSHEVFGVLLAYPGTRGAVTDHAAVAAGVRERGAVVCATADLLALTLLTPPGEWGADV
                     VVGSAQRFGVPMGYGGPHAGFMAVADDLKRALPGRLVGLSVDAQGDPALRLALQTREQ
                     HIRREKATSNICTAQVLLAVMAAMYATYHGPDGLRAIATRVHRLTAAARAALVSHGFS
                     TTAETFFDTVTLRIPGDADRIVAQALGAGLNLRRVSADEVGFSLDETTTTEVVARLLS
                     AFGIELAETDLDLDAPSGLPPALHRRSEFLTHPTFHRYHSETEMMRYLRRLADLDLAL
                     DRTMIPLGSCTMKLNAASEMIPITWPEFAGLHPFAPADQALGYRTMIDELEAALVEIT
                     GYDAVSFQPNAGSQGELAGLLAIRAYHRSRGDTARDVCLIPASAHGTNAASAVMAGMR
                     VAVVATDDHGNVDMDDLRAKVAEHGDRISAAMVTYPSTHGVFEEGIVELCEIVHDAGG
                     QVYVDGANLNALVGLARPGEFGADVSHLNLHKTFCIPHGGGGPGVGPVAVRSHLAPFL
                     PGHGAGRLAGGTGDGVGAVAAAPFGSAAILPIPWMYITMMGDRLRHATELAILSANYV
                     AARLDEHFPVLYKGAGGLVAHECILDLRPIERDTGITNEDVAKRLIDYGFHAPTMSFP
                     VPGTLMVEPTESEGLAELDRFCDAMIAIRGEIARVASGEWPAEDNPLVNAPHTARVVG
                     GDDWDHPYPRSVAGWPAQSAACTDGSDGVPRSESDWKYWSPVGRVDGGYGDRNLVCSC
                     PPVVDSL"
     gene            complement(10003..>10385)
                     /locus_tag="PLZ72_10845"
     CDS             complement(10003..>10385)
                     /locus_tag="PLZ72_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNA36837.1"
                     /translation="GHELGPGITPLNAGLEWVVSWTKGDFRGRAALAAQKEAGVTPVL
                     RGLATEGRRPPRADQVVLRAGPDGSAVPVGRVSSGNFSPTLGHGIALAFVDPSVSEGD
                     DVLLDARGQSVPATCVGIPFLPRS"
BASE COUNT         1457 a         3963 c         3538 g         1427 t
ORIGIN      
        1 tggagcggtc gtgcctgccc tcccccgccg ccgacgagcc cgccgggccg atccccgagc
       61 ggtcgaccgc tccgcgctcg accgtcagct cggcgacctc atcgacgagc actccgatgc
      121 ggtgttccgt ctggcggtcg gcatcctgca cgaccgccag ctcgccgagg acgtcgtcca
      181 ggagacgatg atgcgggcgt ggacgtcgat ggcggggttc cgcggcgact cctcggaacg
      241 cacgtgggtg ctgcgaatcg cccacaacac ggccatcgac gcgctgcgcc gtcggcgcga
      301 ccaggccacc gctccgcacg acctgccaga gagcaccgac cgcagcgacc cggcggaccg
      361 ggcgaccggt cgggaccgcc tggccgcggt cgggcgggcg ctggccgggc tcgacgagac
      421 cagccgcgcc gtcgtggtgc tccgggaggt cgagggcatg agctaccagg agatcgccga
      481 cgcgctcgac gtgccggtac ccacggtcaa gacccgcctg ctgcgggccc gacgggcgtt
      541 gcaggccgtc gaccgcgacg aggaggacac atgagcaccc atcgcgaccc cgacgacgtc
      601 gagccgcccg acacccactc ggacgccgag ctcgaccggg agctggcccg cggggacgag
      661 ctgctcggcg cctcgttgcg gagcctgctc tccccgccct ccgacataga ggagcgggtc
      721 gcggaccagg tgtcccagca gctcatcggc cggtccgccg cgggagccgc gctcgacctg
      781 ctgggtttgg gggtgcggac cttcatgatg ttcctcagcg aggacccctc cggtgccgac
      841 agggaccggg agcgcccgga cgacgggccg aaccgcgacg agcaggaccg gagggccacc
      901 gcatgaacaa cgggatctgg gcgctggtcg ccgtgtgcag cgggctcctc ctgggcgagc
      961 tcggcggccg gctcgtccgc gccgcccgcg gtggcccgga ccgaccggag tcggtgcgca
     1021 gcaccgctcg ggccgtcggg tcgctcgtgt tctggagctt caccgccgtc gggttggtca
     1081 tcgccgtcgg ggcgctcgac atcgaggcgc tcgaggacat gggtgagacg atctcggact
     1141 ccctgcccaa cctgctgctc gccgcggtga tggtgatcgt cggctacgcc gtcgccatcg
     1201 cggtggccgc ggcggtcggc cagtcggccc gtcgggcgag cggcgtccgc caggagaacc
     1261 tcgaacgggt cctgcggatc ctgatcacgg tgatcgccgt gctgctcgcc ctgcaccaag
     1321 ccggggtgag cgacgggatg gtcgcggtgc tggtcgtgat cgtcatggcc gtccctgccg
     1381 tcgccgccgc gctgctgtcg gccttcggag gccgggaggt ggccgcccag ctggctgccg
     1441 gccgggcgct acgggcgcag ctgcgcgacg ggtggacgct gcgctgcgag acccccgacg
     1501 gcgagcccgt gaccgggcgc atcatccggg tgcacccgac gacggtcgag atcgagagcg
     1561 ccgacggcgg ccggggccac gtcccgcacg gctacctgct ggcacgcccg ttcaccgtca
     1621 gcccctgagg ttccaccggc cgaccttcgg ccggagcgac cgatctccgg gcgctccgtc
     1681 ccgagggctc tgcgggccca cgacgtccgc cggaaagaat ttccgtcatc ggccgcgact
     1741 ttcgcgaccc ggacacgtca ctacacatgt acgccgctga tgcggcggga cgaccgggtc
     1801 ggctgccatc gccccggaca catccatcgc actcccgtct gcctgctggt gctgcaacag
     1861 gaacaccgag tgcccgtcga tccgggggac ccgcacaggg tcccccgggc cgcgtccggg
     1921 cctacgatcc gccgatggtc cgccacgtcg tcctgctcac cttccggccg aacaccccgc
     1981 gggcggacgt cgacgcggtc gccgacgcgc tgcggacgct cccgggccgg atccccgagc
     2041 tgcgcgggta cgacgtcggt gtcgatcttg gcctggccgg agccgatggc ggcaacgcgg
     2101 atctcgcggt ggtggccgac accgacgacg tcgccggcta cgaggcctac cgcgaccacc
     2161 ccgagcacca gcggatcatc accgagatga tccggccgat cctggcgtcc cgggtcgcgg
     2221 tgcaacacga gcgatgactc ctcacccggg ccgtagtccg aaccacgttc gggttgcaag
     2281 aggtttttcg tgacagcagt caccccgtgt cgtatggtgc cgagcgtcaa ccaccgaggt
     2341 ggttgacccg gtcgatgcgc tcgggttccc cgagggctcc cggtgtccgg caaggccgtg
     2401 tggaatcggc acggggggtt tcgattccac acggcctccg gccgttctag ggagcctccg
     2461 gccgttctag ggagcctccg gccgttctgg ggagcctccg gccgttccca cgagcgtcgg
     2521 ggcgttgtcg ggcagactgc gacggtgacc gtgaagaagt tgaccggaac cgacgccttc
     2581 gtcatcaccg accttcccga cgccgagacc gcggacggtg tggtgcgttg cgcccgcaag
     2641 gtcctccagg acggggctcg cacgatggcg cgatcgcgca cctactcgtg ggcgctgctc
     2701 gagcagcaga tcagcggggc gtccgccggc gtcaacgcga ccgacgaccg agacgcggcg
     2761 ctccgcacgt tcgtcgatga gctgagcggc gagataggga gcggacgact cgcgctcgac
     2821 ccgggcaagg gtgtgaccac cgaggatctg gccgcgtggg cggagctcga ccgtcgttct
     2881 cccgagcaca ccggtcgtcg tgacgacctg ctggccgccg ggatcgtcgc cgccgcggac
     2941 gtggccgtcg gtggggttgc gggtgcgtcg gtggtcatcg agggcgccgg cgcctcgggc
     3001 tccgccatcg ccgccgcgtt cggcgccgcg gacgcagagg tggtgtcgac cggcgacggg
     3061 cccgacgtgc tgagggacgc cgctgatgtc ctggtctgcg gctccaggct cggcctcgtc
     3121 gaccaccagg tggccgccgc gctcccccag cgggccgtcg tcccctgcgg cgtcgcaccc
     3181 gtcaccgcca agggcctggc cgtcgccacc gggcgcggga tcgtggtcgt gcccgacttc
     3241 ctgtccctga tcggacctct gctggggttc cgtcccgacg ccgggtccag caccgagtcc
     3301 ctcctggtgg atgccgccga gcgggtgcgc accgtcctga ccgacgtgat cggccacgac
     3361 gagggcgcct acctgggagc ctgctaccgg gccgaggcgt tcctgaggac atggcaggac
     3421 acccttccgt tcgggcgccc cctggcctga gcagcatgac ggccacaccg aatggacagc
     3481 aacaccgtga gcaccctgga acaccccgaa ccgaccgtcg accttcccgc ccaacccgac
     3541 ggggtcccgt ggccgaccgg gagctggccc gagggacctc agctcagcgg ggaccccgat
     3601 cggttggccg ccatcctcga cccggtcttc gccaccgagg agaccccgga gctcgcccgg
     3661 tccctggcgt tcgtcgccgt ccagggcggc cgcgtcgtcg ccgagcgata cggccccggg
     3721 gtcggcccgg actcgacgct catctcgtgg tcgatggcca agtccgtcac ccacgccctg
     3781 gtcggcatcc tcgccggtga cggcctgctc gaccccgacg cacccgccgc ggttccggag
     3841 tggagcgacc ccggcgatcc ccgccatccg atcacggtcg ccgacctgct ggccatgcgc
     3901 tcgggactgg cgttcgtcga ggactacctc gacgacagcg tgtcggactg cctcgagatg
     3961 ctgtggggat ccggggcgga cgacaccgcc cactacgcgg cgtcccgacc gctcgagcac
     4021 ccgatcggca cggtgttcaa ctactcgagc ggcacgacca acatcctgag ccggctgatc
     4081 ggatcggtcg cggtccgcga cgtcccgtcg ctgctgcggg aacggctcct cgacccgctc
     4141 gggatgtcct ccgcaaccgc ccagtgcgac gcctccggga cgtgggtcgg gtcgtcctac
     4201 ctgttcgcga cggctcggga cttcgcccga ttcgggctcc tgtacctgcg cgacggcgtg
     4261 tgggagggcg agcggctgct gcccgagggg tgggtcgact cggctcgcac gttgcggtcg
     4321 atcgacgacg agaacggctg gggctacggg caccactggt ggatccgcgt cggcgacgac
     4381 cgcggcacct tctgggccaa cggatacgag gggcagatga tctgctgcgt cccggccctc
     4441 gacctggtcg tcgtgcggct gggcaagacc ccgagcaccc tgcgaccgca cctcgaacgg
     4501 ttcttcttcg acgtcgtgga ctgcttcgcc ccctgaccct gacccccact ccccctccct
     4561 tcccgttctg ttcgttcgtt cacaacgcct gcgttgtgaa cgaacgaaca gaacgggaag
     4621 gggcgagcag aacggggcct agtctcgggc gatggtcgac caccgcccgt cgcgtccgcc
     4681 gcgcttcacc ttccacgagt ccgactcgct cgccgatgag cgcacgcacc gcaagcagcg
     4741 cctggccgcc gcgttccggc tgttcggacg gttcggattc ggcgagggca tcgccgggca
     4801 catcaccgcc cgcgaccccg agcacgccga ccggttctgg gtgaacccgt tcgggatgaa
     4861 cttccgccag atccgcgcct cggacctgat cctggtcgac cactccggcg aggtggtcga
     4921 gggcgactgg ccggtcaacc gcgccgcgtt cgccatccac tcgcgtgtgc acgccgcccg
     4981 acccgacgtg gtggccgccg cgcactccca cgccgtgttc ggacgcagct tctccaccct
     5041 gggtcgtccc ctcgccccgc tcacccagga cgcctgcgcc ttccacgacg accaggccgt
     5101 cttcgacgac tacaccgggg tggtcgagga ccccgccgag ggcgaccgga tcgccgcggc
     5161 cctcggccgt cgcaagctgg ccatcctgcg caaccacggg ctgctgaccg tcggcaccac
     5221 cgtcgacgcc gcggcgtggt ggttcatcac cgcggatcgc agctgccagg ccgagctcct
     5281 ggcccgggcc gcgggcaccc cgaccgagat cgatcccgcc accgcggcgc tgacccactc
     5341 gcaggtgggc tccgacgccg tgggggcgtt cagcttccac cccctctact gctggatcgt
     5401 cgccgaggag cccgatctcc tcgagtgacg cagccccgct gacgcagccc cgctggcgca
     5461 gcccgaccgg gatccggcct acttgaccgt tcggtcaagt agggtccgga ccatggactg
     5521 gcccctgcgc tgcggcgtct tcatggctcc gttccacccg aacggacaga accccaccct
     5581 cgccctggag cgcgacctcc agctggtgga gcacctcgac cgggtcgggt tccacgaggc
     5641 gtggatcggc gagcaccatt ccggcggctt cgagatcatc gcgtcgcccg aggtgttcat
     5701 cgccgcggcg gccgagcgca cccgccacat cgacctcggc accggggtgg tgtcgctgcc
     5761 gtaccacaac cccctgatga cggctcagcg catcgtgcag ctcgaccaca tgacccgggg
     5821 acgggtgaag ttcggcgtgg gccccggagc gctgcccagc gacgcgttca tgctgggcat
     5881 cgacccggcc gaccagcgtc gacgcatgga ggaggcgctc gaggtcatcc tgcacctgct
     5941 gcgctccgac gagccgatca ccgtcgagac cgactggttc accctccgcg acgcccgcct
     6001 gcacctgcgg ccctacaccc acccgcaccc cgaggtcgcc gtcgcggcga tgatctcgcc
     6061 gtcgggcccc cgggccgcgg gacgcttcgg ctgctccctc ctgtcgatcg gggccaccca
     6121 gcgcgccggg atcgacctgc tcggccaggc ctgggaggtc atggaggagc gctccgccca
     6181 gttcggcaac atcgccgacc ggcgccagtg gcggttggtc acgctcatgc acctggccga
     6241 gacccgcgag caggcggtcc gcgacgtcga gttcggcctg gccgactacg cccgctactt
     6301 ccgcgaggtc gccgccctgc ccatcttccc cgacgccccc atcgagacga tcgtcgacac
     6361 cctcaagagc tccggcgccg ccgtcatcgg cacccccgac gacgccatcg agctgatcca
     6421 gcacctgatc gacgagtccc acggcgggtt cggcacgctg ctgctgcaga gccacgagtg
     6481 ggccaacccc gaggccaccc gccgcagcta cgagctgttc gcccagtacg tgctgcccca
     6541 cttcgacccc cacgtcgcgt cgaccaccgc gtcgatggag tgggtgatgg ccaaccggcc
     6601 cgagcagctc ggcgcggcaa tgaacgccat cggagccgcc atccagcagc acgccgagga
     6661 gaaggcggcg gccccaccca gctgaggcac ggcggccatg tcaccggtga gccggcgcgg
     6721 ctagcctcgg atccacaacc gacgccgcat cccgtccggg agagctcggt ccccgatccg
     6781 cccacgggcg ccgtcggggt cgacgccgaa ggagcaaccg cccccggaat ctctcaggcc
     6841 cacgtaccgg gcgggcaggc accgctggag agcagcccac cgacgacgct gggctcaccg
     6901 acggggaaag tcacgccgtc acccgcgggt cccggcacga cgaagctctc aggtcacgga
     6961 acagcggggg gccgaagcct cccaccgtcc gcgaccccgg agaccccgtg agcaccgacc
     7021 ccacacccga gcagccgttg aacgggaccc cgaccagcgg tcccggaccc gacgaagggg
     7081 gcttcgccga acgtcacctc ggccccgacg ccgacgcggt cgcccacatg ctcgcgacgc
     7141 tcggacggac cgacctcgac tccctgatcg acgacgccgt gcccgcgtcg atccgccaga
     7201 gccgaccgct cggcctgcag gcgatcgccg atgagagcga ggcgctcgag cagctccgac
     7261 ggatcgccgg caccaacacc gtggtcacga gcctgatcgg ctgcggctac cacgacaccg
     7321 tcacgccgcc ggtcatcctg cgcaacgtgc tcgagaaccc cgcctggtac accgcgtaca
     7381 cgccgtacca gcccgagatc tcccagggcc gcctcgaggc gttgctcaac ttccagacga
     7441 tggtcgccga gctgaccggg ctcgacctcg ccaacgcctc cctgctcgac gaagccaccg
     7501 ccgcggccga ggccatgacc ctggcccggc gacgctccaa ggtcgccggc gacacgttct
     7561 tcgtcgactc cgagtgccac ccccagacga tcgaggtcgt cgagacccgg gccgagccgc
     7621 tcgggatcaa ggtggtcacc ggcgacccgg cccgcgacct cgacagccac gaggtgttcg
     7681 gggtcctgct cgcctacccc gggacccgcg gcgcggtgac cgaccacgcc gcggtcgccg
     7741 ccggtgtccg ggagcggggt gcggtggtgt gcgcgacggc ggacctgctc gccctcacgc
     7801 tcctcacccc gcccggcgag tggggagccg acgtcgtcgt cggctccgcc cagcgcttcg
     7861 gcgtcccgat gggctacggc gggccgcacg ccggcttcat ggcggtggcc gacgacctga
     7921 agcgcgctct gcccggccgc ctggtcgggc tgtcggtgga cgcccagggc gacccggcgt
     7981 tgcgtctggc gctgcagacc cgggagcagc acatccgccg cgagaaggcc acatccaaca
     8041 tctgcaccgc gcaggtgctg ctggcggtga tggccgccat gtacgccacc taccacggcc
     8101 ccgacgggct gcgggcgatc gccacccggg tgcaccgcct gaccgcggcg gcgcgggccg
     8161 ccctggtgtc gcacggattt tccaccaccg ccgagacgtt cttcgacacc gtcaccctcc
     8221 ggatccccgg cgacgccgac cgcatcgtcg cccaggccct cggcgccggg ctcaacctgc
     8281 ggcgggtgtc ggccgacgag gtcggcttct ccctcgacga gaccaccacc accgaggtcg
     8341 tggctcgcct gctgtcggcg ttcggcatcg agctcgccga gaccgacctg gacctcgacg
     8401 ccccgtccgg gctgccgcct gcgttgcacc gccgcagcga gttcctgacg cacccgactt
     8461 tccaccgcta ccactccgag accgagatga tgcgctacct gcgccgtctg gccgacctcg
     8521 acctggcgct cgaccgcacg atgatcccgc tcggcagctg caccatgaag ctgaacgcgg
     8581 cgtcggagat gatcccgatc acctggcccg agttcgccgg gctccacccg ttcgcgccgg
     8641 cggaccaggc gctcggctac cggaccatga tcgacgagct cgaagcggcg ctcgtcgaga
     8701 tcaccggcta cgacgccgtc agcttccagc ccaacgcggg cagccagggc gagctggccg
     8761 gcctgctcgc gatccgcgcg taccaccgct cgcggggcga caccgcccgt gacgtctgcc
     8821 tgatcccggc gtccgcccac gggacgaacg cggcgtccgc ggtgatggcc ggcatgcgcg
     8881 tcgcggtggt cgccaccgac gaccacggca acgtcgacat ggacgacctg cgggccaagg
     8941 tcgcggagca cggcgatcgc atctcggcgg ccatggtcac ctacccctct acccacggcg
     9001 tgttcgagga ggggatcgtc gagctgtgcg agatcgtcca cgacgccggc ggccaggtgt
     9061 acgtcgacgg cgccaacctc aacgcgctgg tcggcctggc ccgccccggg gagttcggcg
     9121 ccgacgtcag ccacctcaac ctgcacaaga cgttctgcat cccccacggc gggggtggcc
     9181 ccggcgtggg tccggtcgca gtgcggtccc acctcgctcc gttcctcccc ggacacggtg
     9241 caggtcggct ggccggcggg accggcgacg gcgtcggagc ggtggccgcg gcgccgttcg
     9301 gatcggcggc catcctgccg atcccgtgga tgtacatcac gatgatgggc gaccggctgc
     9361 gccacgccac cgagctcgcg atcctgtcgg ccaactacgt cgcggcgcgc ctcgacgagc
     9421 acttcccggt cctctacaag ggggccggcg ggctggtcgc acacgagtgc atcctcgacc
     9481 tgcgaccgat cgagcgcgac accggcatca ccaacgagga cgtggccaag cgcctcatcg
     9541 actacggctt ccacgctccg acgatgtcgt tcccggtgcc ggggaccctg atggtcgagc
     9601 cgaccgagtc cgagggcctg gccgagctgg atcgcttctg cgacgccatg atcgccatcc
     9661 gcggcgagat cgcccgggtc gcctccggcg agtggcccgc cgaggacaac ccgctggtca
     9721 acgcgccgca caccgcccgg gtcgtcggcg gtgacgactg ggatcacccc tatccccgat
     9781 cggtcgccgg ctggccggcg cagtccgcgg cctgcaccga cggctccgac ggcgtgcccc
     9841 gcagcgagag cgactggaag tactggtcgc cggtcgggcg cgtcgacggc ggctacggcg
     9901 accgcaacct cgtctgcagc tgcccgccgg tcgtcgactc gctctgagcg gcgaccggga
     9961 gcgcgtggac ggagccgggg cctcgcgacg ggcggcgccg gttcacgaac gcggcaggaa
    10021 cgggattccg acgcaggtgg cgggcaccga ctggccccgc gcgtcgagca ggacgtcgtc
    10081 gccctccgac accgacggat cgacgaacgc cagggcgatg ccgtgaccga gggtgggtga
    10141 gaagttgccc gagctgaccc gtccgaccgg caccgccgag ccgtcgggtc ccgcccggag
    10201 cacgacctga tccgcccgcg gcgggcgtcg gccctcggtg gcgaggccgc ggaggaccgg
    10261 ggtcaccccc gcctccttct gggccgccaa cgcggcgcga ccccggaagt cgcccttggt
    10321 ccacgacacc acccactcga gcccggcgtt cagcggcgtg atccccgggc ccagctcgtg
    10381 gccgt
//
LOCUS       DAOQOD010000108        10259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2352_length_10259_cov_14.960702, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000108 DAOQOD010000000
VERSION     DAOQOD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2352_length_10259_cov_14.960702"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..408)
                     /locus_tag="PLZ72_10850"
     CDS             complement(187..408)
                     /locus_tag="PLZ72_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36838.1"
                     /translation="MPVDHALRPTPRGVEVTASEIGFDCDERRCLTASAERDDRTWTV
                     PLLDIEITADQHRVARLVAAYRRWLGIDR"
     gene            410..691
                     /locus_tag="PLZ72_10855"
     CDS             410..691
                     /locus_tag="PLZ72_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36839.1"
                     /translation="MTSDLVDAIDRLDGLKMFALAVIIGTAVRGLRSVGLVGRPMAVT
                     GLFATVALAVSGAGYLLDVAPLEAAAFVSLPLLLVWVGTLGVRVARTAR"
     gene            691..1257
                     /locus_tag="PLZ72_10860"
     CDS             691..1257
                     /locus_tag="PLZ72_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNA36840.1"
                     /translation="MPRAGLSRERVVEEAASVADQVGFDRLTMAAVAERFGVALPSLH
                     KHVGGIDALRRDLAVHGMAVLADTLRDAAVGRVRGDALTAVATAYRQFATDRPGLYAA
                     TVRAPAPDDEEHSSVAQTALDVVVAVMRSYGIEGVDAVHALRMLRGALHGFVALDAAG
                     GFGMPESVDDSFDRMVAAFDVILSEQAS"
     gene            1254..1559
                     /locus_tag="PLZ72_10865"
     CDS             1254..1559
                     /locus_tag="PLZ72_10865"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HNA36841.1"
                     /translation="MRPGVSAVRFATEVAALAALAVAGLAIAWPLALALPAAFAFVRG
                     WWIAPKAHSRLADPSRFAVEIVLFVAAGGTLAATGSVPLGLLLTAVAVAAAIAIRRE"
     gene            complement(1837..2436)
                     /locus_tag="PLZ72_10870"
     CDS             complement(1837..2436)
                     /locus_tag="PLZ72_10870"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNA36842.1"
                     /translation="METATVLAAVALIAFAVASGCLIQLHRVAPEVDPLRDAVSEYGA
                     GPHASWYRAQVLLVGLGAAATASGMQSAGLAADGTSWLYVFAASRILIAGFPVDLPGS
                     ETTRTGVVHNLLAAIAFASIAVAASSLGGDLVADPTWSFDSAWYGWLGTIVTVSAVAL
                     AVVWVLPSLRRSVFGLVERCWYAATLAWLGLTGIGLLMR"
     gene            complement(2478..3041)
                     /locus_tag="PLZ72_10875"
     CDS             complement(2478..3041)
                     /locus_tag="PLZ72_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNA36843.1"
                     /translation="MALLRGINVGGRNKVPMAELRSALTERGLSDVRTYIQSGNVVFG
                     SDLDPAGLEVLVADVVRSSFGVESPTVVRSAADMRAVVESAPSGFGGEPDSYHYDVIF
                     LKDPLSAPDAMAVVKVRDGVDRAWEGAGCLYFARLSARRTQSRLSSIIGTPEYGLMTI
                     RNWRTVSTLIGMVGRSEVDRSDGSTSC"
     gene            complement(3140..4066)
                     /locus_tag="PLZ72_10880"
     CDS             complement(3140..4066)
                     /locus_tag="PLZ72_10880"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNA36844.1"
                     /translation="MTDAPRPGSPDRFTGLVGCRVAIQLAAMGAVSTPALAGAVAGAG
                     GLGMLSGTALPPDELDRQLSSAVAAAAGGGGAVGVGFLMPFLDVAAFEAAASAARLVE
                     CFYGDPDADLARRAHDAGALLSWQVGSLDEALRAVDAGCDLVVLQGTEAGGHVRGASG
                     LIDLVSTARPRLEVPVIAAGGIGTPERATEALAAGADAVRVGTRFLAATEADVHPGYL
                     AALVAAGGDDTTITEAFSGGWPNAPHRVLISCVTASDADPLTRSVICPTRGFTGDVTG
                     AALYAGRSVGAVTGETTAAAIVDEFAAALGRR"
     gene            complement(4136..4534)
                     /locus_tag="PLZ72_10885"
     CDS             complement(4136..4534)
                     /locus_tag="PLZ72_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA36845.1"
                     /translation="MSTLLADGNLLIALVVVDHVHHDRAVEWFTTEEPTLATCPITEG
                     TLLRFLIREGGSASTAIGVLDAIRAAPWHRFWADDIPYEAAQLGGVIGHRQVTDAYLV
                     ALARHHGGRVATLDKGLAALHGGDVLLLGA"
     gene            complement(4531..4761)
                     /locus_tag="PLZ72_10890"
     CDS             complement(4531..4761)
                     /locus_tag="PLZ72_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36846.1"
                     /translation="MRTTIDLPDDLHQLARQIAHESGRSMSEVVAELIRLGLQPNAGR
                     PAAPVTGPRGLPTVRLGRPVTSEDVRSLDDDG"
     gene            complement(4814..5653)
                     /locus_tag="PLZ72_10895"
     CDS             complement(4814..5653)
                     /locus_tag="PLZ72_10895"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNA36847.1"
                     /translation="MYSIGDLSQLGGVSPRMLRHYHKLGILVPAEVDDATGYRRYDES
                     QLADLLQVLVLKDLGFALTDVKRVVEGGVSPSELRGMLLLRRTEVESELNETNARLER
                     VEAHLTRLESQMNEQSAPDVAVTTKRLPAVHLAVASAISPSFASADVGPVIQPLYPQL
                     FAALDAAGVEVAGPSIAYYDDTADGGIGVHAGFPIDESVTQVPGLEVVDLPEVGLAAT
                     AVHHGDMSTVDVDTVDAILDWVHANGFRTTGYSRENYLDCSGELAQWQTEIQFPIAPD
                     PDR"
     gene            complement(5807..6193)
                     /locus_tag="PLZ72_10900"
     CDS             complement(5807..6193)
                     /locus_tag="PLZ72_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA36848.1"
                     /translation="MVSPSALDHIVLVVADVDTSLEWYMRHAGLESVNVEAWRNGEAF
                     FPSLRVNEATIIDFVEGETGRGRGHLDHVCFVVSDEDLAEIASDPDLEVVDEGQRSGA
                     RGMGRSVYVRDPDQLLVEFRSYPPPG"
     gene            complement(6237..7181)
                     /locus_tag="PLZ72_10905"
     CDS             complement(6237..7181)
                     /locus_tag="PLZ72_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNA36849.1"
                     /translation="MAGPSERMLALLSLLQARRDWPGHVLAQRLDVTTRTVRRDVDRL
                     RALGYRIESTKGPDGGYRLAAGSELPPLLFDEEQAVAIAVGLQHAAASGVDVGDAADR
                     ALATVRQVMPSHLRNRVDAVRFSGAVVEEMVDPDVLEAASRAVHDRTTMRFDYGDAAR
                     SRHVEPHGLVARGGRWYLVAWDLDRRDWRIFRLDRMTPSRTPGPRFTPRAIPTGDAAT
                     FVAARSKGSTDDDRWPCTGRFEIDLPAAELAPWIRDGKVEEIGPGSSRVTMGSWSCAG
                     LLAAVVRFDAPFRVLAPAELIAEAQLLGERLREGSSSA"
     gene            7280..7726
                     /locus_tag="PLZ72_10910"
     CDS             7280..7726
                     /locus_tag="PLZ72_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA36850.1"
                     /translation="MAISTATHLNFAGNARDALDFYRSVFGGHVVVTTYGEVGMPAEA
                     PDAHKVVFGQLVGDDGFCVMAYDVPGRVEPFTGTTRREHGTTITDQPFFVSLSAGTLD
                     EIALRWERLADGATIVEPLAASAWSPGFGMLTDRFGVTWAFTVVPT"
     gene            7797..8489
                     /locus_tag="PLZ72_10915"
     CDS             7797..8489
                     /locus_tag="PLZ72_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNA36851.1"
                     /translation="MTPSIGRPGGSAERPAVFFSGPEEFRSWLEANHDTADELWMGLN
                     RRHVEDRGLTWEDAVPEALAYGWIDSKAERIDDDRRRQRWTPRRKGSNWSVINIAIAE
                     DLIAQGRMMPSGLAAFEARREDRSGVYTHENPAQELTVEHAALLAADPRATAFWEAAP
                     PYYRRVCVGWVNGAKQQTTRDRRMAQLVQDSANGQLIKSQRYGDTPTWVTRARAAADG
                     VASPPLFPAPAR"
     gene            8562..8789
                     /locus_tag="PLZ72_10920"
     CDS             8562..8789
                     /locus_tag="PLZ72_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNA36852.1"
                     /translation="MRVTEKGQVTIPKDIRDALGIGAGTEVEFERRRDSIVIRKSSRG
                     RSRGQQLAERLRGRGDVEMTTDEIMALTRGV"
     gene            8779..9204
                     /locus_tag="PLZ72_10925"
     CDS             8779..9204
                     /locus_tag="PLZ72_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNA36853.1"
                     /translation="MASDGVAGTLVDSNVLLDILTDDPRWADWSAGALADAAEAGPIF
                     INPIIYSEVSVRFTTVEALDAALPSRDYRREPIPWAAAFLAGKVFVDYRRNTGTKATT
                     LPDFFIGAHAAVAGMDLLTRDAGRYRTYFPTVRVIAPDS"
     gene            complement(9326..>10259)
                     /locus_tag="PLZ72_10930"
     CDS             complement(9326..>10259)
                     /locus_tag="PLZ72_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA36854.1"
                     /translation="DAGAVRVGHGGTTLTSTTEPAEHHVRVGDIDLAYLAAGGSGPLA
                     VCLHGFPDTAHTWTHLLPALADAGFRAVAPWLRGYAPSGLDPNDHYQIGASVADVVAM
                     HEALGGDADAVLIGHDWGARIATGASVLAPDRWSRLVTASVPPSAAVAQGFLTYPQLR
                     RSWYMFFFQNPLAEIAVAMNDLEFIDHLWQDWSPGLDAPEHVERVKESLRDPANLHAA
                     IEYYRATLNPAEHTTADYQAEEAAIDGTPTQPHLYLHGESDGCIGVEVGRLAEPLLTV
                     PGSRLEVIEGAGHFLQLEQPERVNRSILEFLASR"
BASE COUNT         1546 a         3578 c         3614 g         1521 t
ORIGIN      
        1 cgcgttgctc tctcggctcg tgaccatgca tccgtcgagg atgcgggacg cgcacttgac
       61 caccgtggcg agcttgatcg gggcgacgga tgaatcgacc atcgccgcgc tacgtcgggc
      121 caaggccgca cagacggccg acctgatgga ggcgttgttg aaaatgtcct gaccgcccca
      181 ccacttctat cgatcgatgc cgagccagcg gcggtaggcg gcgacgaggc gtgcgacccg
      241 gtgctggtct gcggtgatct cgatgtcgag gagcgggacg gtccaagttc ggtcgtcgcg
      301 ttcggccgac gcggtgaggc agcggcgttc gtcgcagtcg aaaccgatct ccgacgcggt
      361 gacctcgact cctctcggtg tggggcgcag cgcgtggtcg acgggcacca tgacatccga
      421 cctcgtcgac gcgatcgatc gactcgacgg cctcaagatg ttcgccctcg cggtgatcat
      481 cggtaccgcc gtccgtggtc tccggtcggt cggactcgtg ggccgaccca tggccgtgac
      541 cggcctgttc gccaccgttg ccctggccgt gtcgggcgcc ggctacctgc tcgacgtcgc
      601 gccgctcgag gccgccgcgt tcgtctcgct ccctctcttg ctcgtgtggg tcggcaccct
      661 gggcgtccga gtcgcacgca cggcgcgctg atgccccgcg ctggactgtc cagggagcgg
      721 gtcgtcgaag aagccgcgtc cgtcgccgac caagtgggat tcgatcggct caccatggcg
      781 gccgtcgccg agcggttcgg ggtcgcgctg ccctcgctgc acaagcacgt cggcggcatc
      841 gacgccctgc gccgggacct cgccgtgcac ggcatggcag tcctcgccga cacgctgcga
      901 gacgccgcag tgggacgggt ccgaggcgat gccctcactg ccgtggcgac cgcctaccgg
      961 cagttcgcga ccgaccggcc cggcctgtac gccgccaccg ttcgcgcccc ggcgcccgac
     1021 gacgaggagc actcctcagt ggcccagacc gcgctcgacg tcgtcgtagc agtgatgcgc
     1081 agctacggga tcgaaggagt cgatgccgtc cacgcgctcc gcatgctgcg aggcgcgctt
     1141 cacgggttcg tcgccctcga cgccgccggc ggtttcggca tgcccgagag cgtcgacgac
     1201 agcttcgatc gcatggtcgc cgccttcgac gtgatcctgt ccgagcaggc gtcatgagac
     1261 ctggtgtcag tgcggtcagg ttcgcgaccg aggtggcggc gctcgccgcg ctggccgtcg
     1321 ccggcctcgc catcgcctgg ccgctcgcgc tcgcgctacc agcggcgttc gccttcgtgc
     1381 ggggctggtg gatcgcaccc aaggcccaca gcaggctggc cgatccatct cgcttcgcgg
     1441 tggagatcgt gttgttcgtc gccgccgggg gcacgctcgc cgcaaccgga tccgtgccgc
     1501 tcggactcct gctcaccgct gtcgcagtcg cggcggccat cgcgatccgc cgtgagtagg
     1561 ggcatggata tcgagtggag cgcacccgcc ggcgcgggtc cgacgcaccg tcgacccagt
     1621 ggacaccgct gccctcccgc gacggctggg tcgatccggg tgttccgttg taggggccac
     1681 gggttctgag acacccagca cgatctcggt cccgatgcag gcgccggagg ggatcttcgc
     1741 gaacggttta cgactggccc gacatccagc tcgaggctcg tggcatccac atcgtgtccg
     1801 ggccggtcgc ccatccgctc ggcccggcgg acgcggtcaa cgcatgagca gtccgatccc
     1861 agtgagtccc agccaagcga gcgtcgccgc gtaccagcac cgttcgacca acccgaacac
     1921 gctgcgccgc aggctcggca gcacccagac gacggcgagc gcgacagccg agaccgtcac
     1981 gatcgtcccc agccagccgt accacgcact gtcgaagctc catgtcggat cggcgacgag
     2041 gtcgccgccg agggagctgg cggcaacggc gatcgaggcg aacgcgatgg cggcgagaag
     2101 gttgtggacg acaccggtac gggtcgtctc ggaccccggc aggtcgacgg ggaagccggc
     2161 gatcaggatc cggctcgccg cgaacacgta gagccacgac gttccatcgg ccgcaagccc
     2221 ggcggactgc atcccgctcg ccgtggcggc cgctcccaag ccgacgagca gaacctgggc
     2281 ccgataccac gacgcgtgcg gacccgctcc gtactcgctc acggcgtccc ggagcggatc
     2341 cacctctggg gcgactcggt ggagctggat cagacaaccg ctggcaactg cgaacgcgat
     2401 cagcgcgact gctgccagga cggttgcggt ctccatgtcg agtctccctc gtcagggaat
     2461 ctgatcgtcg actcggtcta gcagctggtg gatccgtcgg agcggtcgac ctcggacctc
     2521 ccgaccatcc cgatgagcgt cgacacggtt cgccagttgc ggatggtcat cagcccgtac
     2581 tcgggggtgc ctatgatgct cgacagccgg ctctgggtgc gccgggcgct cagccgggcg
     2641 aagtacaggc agccggctcc ctcccacgcc cgatccaccc cgtcgcgcac cttcacgacc
     2701 gccatggcgt caggtgcgct cagcgggtcc ttcaggaaga tgacgtcgta gtggtacgag
     2761 tccggttcgc cgccgaaccc cgacggtgcg gactcgacga ccgctcgcat gtcggccgcg
     2821 gatcgcacga cggtgggcga ctcgacgccg aagctcgacc ggaccacgtc ggcgacgaga
     2881 acctccagcc cggccggatc gaggtcggat ccgaacacga cgttgccgga ctggatgtag
     2941 gtccgcacgt cgctgagccc gcgctcggtc agcgccgatc ggagctcggc catgggcacc
     3001 ttgttgcgtc cgccgacgtt gatccccctg aggagtgcca cgaacctgtc cgagctgtcc
     3061 gggctcatcg gtcgagtctg ctccgagcct gacggcgacg acgcccgcgc ggcgatgagt
     3121 gggagcggtc cacggggtgt caccgccgcc cgagggcggc ggcgaactca tcgacgatcg
     3181 cggccgcggt cgtctcgccg gtcaccgcac cgacggaccg cccggcgtac agcgccgcgc
     3241 cggtgacgtc accggtgaac ccacgcgtcg ggcagatcac cgagcgggtc aacgggtccg
     3301 cgtcggacgc ggtgacgcac gagatcagca cccggtgcgg cgcgttcggc caaccgccgg
     3361 agaacgcctc ggtgatcgtg gtgtcgtcgc cgccggcggc gaccagcgca gcgaggtacc
     3421 cggggtgcac gtcggcttcc gtcgcggcga ggaatcgggt gccgacgcgc accgcgtccg
     3481 ctccggcggc gagggcttcg gtggctcgct cgggtgtgcc gatcccgccg gcggcgatga
     3541 caggaacctc cagccgcgga cgtgccgtgc tcacgaggtc gatcaacccg ctggccccgc
     3601 ggacgtggcc gccggcctcg gtgccctgca acacgaccag gtcgcacccg gcatcgacgg
     3661 ctcgcaacgc ctcgtcgagg gacccgacct gccacgacag caacgccccg gcgtcgtggg
     3721 cccgtcgcgc caggtccgcg tcggggtcgc cgtagaagca ctcgacgagc cgggccgcgc
     3781 tcgcggcggc ctcgaacgcg gcgacatcga ggaacggcat caggaacccg acgccgaccg
     3841 caccgccacc acccgcggca gcggcgaccg cggaggacag ctggcggtcc agctcgtcgg
     3901 gcggcaacgc cgtgcccgag agcatcccca gtccgcctgc cccggcgacc gcacctgcga
     3961 gggcgggtgt gctcaccgcc cccatcgccg cgagctggat cgcgacgcgg catccgacga
     4021 gcccggtgaa cctgtccggc gaccccggcc ggggtgcatc ggtcatcggt gaaacctacc
     4081 cggcggacga cgggtcgggt gtgggaactc gacggactcg acagcgctcg gcggctcaag
     4141 cacccaggag gagcacgtcg ccaccgtgaa gtgcggccag ccccttgtcg agcgtcgcca
     4201 cccgcccgcc gtggtgtcgg gcgagtgcca ccaggtaggc atcggtcacc tggcggtgcc
     4261 cgatgactcc accgagctgc gcagcctcat aggggatgtc gtcggcccag aagcgatgcc
     4321 acggtgcggc ccggatcgca tcgagcacgc cgatggccgt gctcgcagag ccgccctccc
     4381 gaatcaggaa cctcaacagg gttccttcgg tgatcgggca cgtcgcaagg gtgggttcct
     4441 cggtggtgaa ccactcgacg gcgcggtcgt ggtgcacgtg gtcgacgacg accagtgcga
     4501 tgagcaggtt gccgtcggcg aggagtgtgc tcacccgtca tcgtccagcg agcggacgtc
     4561 ctcggaggtc accggtcgac cgagtcgcac ggtcggcagc cccctcggac cggtcacggg
     4621 agccgcgggg cgacccgcgt tcggctgcag cccgagtcgg atcagctcgg cgaccacctc
     4681 gctcatcgag cggccgctct cgtgcgcgat ctgccgcgcc agttggtgca ggtcatccgg
     4741 caggtcgatc gtcgtccgca tgagtgcatc atcgcatcat gatgctgaga tgtcgattgg
     4801 ccggcgaggc gggtcagcga tcagggtccg gtgcaatcgg gaactggatc tcggtctgcc
     4861 actgcgcgag ctcacccgag cagtcgagat agttctcccg ggagtatccg gtggtgcgga
     4921 agccgttggc gtgcacccag tccaagatcg catcgacggt gtcgacatcg accgttgaca
     4981 tgtcgccgtg atggactgcg gtggcggcga gcccgacctc tggcaggtcg acgacctcga
     5041 ggcccggtac ctgggtgacg gactcgtcga tcgggaaccc ggcgtggacg ccgatcccgc
     5101 cgtcggcggt gtcgtcgtag tacgcgatcg agggtccggc gacctcgact cccgcggcgt
     5161 cgagcgctgc gaacagctga gggtagaggg gttggatcac cgggccgaca tctgccgacg
     5221 cgaagctggg gctgatcgcc gaagcgacag cgaggtggac ggccgggagt cgcttcgtgg
     5281 tgacggcgac gtcgggagcg gactgttcgt tcatctggct ctccagtcgg gtgaggtggg
     5341 cttcgacgcg ctcgagccga gcgttcgtct cgttgagttc ggactccacc tcggtgcgac
     5401 gcagcagcag cattccgcgc agctcggacg gactcacacc gccctcgacg acgcgcttga
     5461 cgtcggtcag ggcgaacccg agatccttca ggaccaacac ctgcaacagg tccgcgagtt
     5521 gagactcgtc atagcggcgg tacccggtgg cgtcgtcgac ctcggcgggc accaggatgc
     5581 cgagcttgtg gtagtgccgc agcatgcgag gcgaaactcc accgagctgc gacaggtccc
     5641 cgatcgagta catgcctgcg acggtacggg ttgacaccgc gtcagagtca agccccgctg
     5701 cagggtagat cctgccgacc gctcacaacg gcggtcatcg tcgatggtcg gcgtcggggt
     5761 cgccgtagaa gggctcgacg attctcgcca cggtcgggtc cgcggactac cccggcggcg
     5821 ggtaggagcg gaactcgacg aggagctggt cggggtcgcg cacgtacacc gatcgtccca
     5881 tccccctggc gcccgatcgc tgcccctcgt cgacgacctc gaggtcggga tcggaggcga
     5941 tctcggcgag gtcctcatcg ctcacgacga agcagacgtg gtcgaggtgt ccgcggccgc
     6001 ggccggtctc gccctcgacg aagtcgatga tcgtggcctc gttgacgcgt agggacggga
     6061 agaaggcctc tccgttccgc cacgcctcca cgttgaccga ctcgagtccg gcgtggcgca
     6121 tgtaccactc gagtgacgtg tcgacgtctg cgacgacgag cacgatgtgg tccagagccg
     6181 acggcgacac catgtgagga ccctagagcg ctcgccgcgg taccggtcgg ctgcgactac
     6241 gcggaactgc tcccttcgcg gaggcgttcg ccgagcagtt gggcctctgc gatcagctcg
     6301 gccggtgcga ggacccggaa cggtgcgtcg aagcggacga cagcggcgag cagcccggcg
     6361 cacgaccacg agcccatcgt gacccgtgac gagccggggc cgatctcctc gaccttgccg
     6421 tcacggatcc agggggccag ctcggctgcg ggcaggtcga tctcgaaccg gccggtgcac
     6481 ggccagcggt cgtcgtcggt ggagcccttc gaccgggccg cgacgaaggt ggcggcgtcg
     6541 ccggtcggga tcgcccgcgg cgtgaaccgt gggccgggcg tccggctcgg cgtcatccgg
     6601 tcaagtcgga agatccgcca gtcccggcgg tccagatccc acgccaccag gtaccagcgt
     6661 ccgccgcggg ccaccaggcc gtggggctcg acgtggcgcg acctcgccgc gtctccgtag
     6721 tcgaagcgca tcgtggtgcg gtcgtgcacg gcgcggctgg ccgcctccaa cacgtccggg
     6781 tcgaccatct cctcgacgac ggcgcccgag aagcgcaccg cgtcgacgcg gttgcgcagg
     6841 tgcgacggca tcacctggcg caccgtggcg agcgcacggt cagcggcgtc gccgacgtcg
     6901 acgccgctgg cggccgcgtg ctggagtccg accgcgatcg cgacggcctg ctcctcgtcg
     6961 aacagcagcg gcggcagctc cgagcccgcc gcgaggcggt acccgccgtc gggtcccttc
     7021 gtcgactcga tccgatagcc gagtgcccgc aaccggtcga cgtcgcggcg cacggtccgg
     7081 gtggtcacgt cgagtcgctg ggcgaggacg tggccgggcc agtcccggcg tgcctggagc
     7141 aacgacagca acgccagcat ccgttccgac ggtccggcca cgacatccat cgttccacaa
     7201 gtagaggaca gaagccgtcc gctatggctg tcaacgtgcc tgctggcaac cccgccgacc
     7261 cccactgtct ggagtgatca tggccatctc gaccgccacc cacctcaact tcgccggcaa
     7321 cgcccgcgac gcgctcgact tctaccggtc cgtcttcggt ggccacgtcg tggtgacgac
     7381 ctacggcgag gtgggcatgc ccgccgaggc acccgacgcg cacaaggtcg tgttcggtca
     7441 gctcgtgggt gacgacgggt tctgcgtgat ggcctacgac gtcccgggac gcgtcgagcc
     7501 gttcaccggc acgacgcgcc gcgagcacgg caccacgatc accgaccagc cgttcttcgt
     7561 ctcgctcagc gccggcaccc tcgacgagat cgccctccgc tgggagcgac tcgcggatgg
     7621 cgccacgatc gtcgagccgc tcgcggcatc ggcgtggagc cccggcttcg gcatgctgac
     7681 cgaccgcttc ggcgtcacct gggcgttcac cgtcgttccg acctgaggcg accgtgtcat
     7741 ggctgaggtc cttcgggtcg atgtgcgctg cggccggaac gcgcgagcct gaggcggtga
     7801 ctccctcgat cggcagaccc ggcggctccg cggagcgtcc ggcggtgttc ttctcggggc
     7861 cggaggagtt ccgctcatgg ctcgaggcga accacgacac cgcggatgag ctgtggatgg
     7921 gcctgaaccg gcgacacgtc gaggatcgtg gcctgacgtg ggaggacgcc gtccccgaag
     7981 cgttggcgta cggatggatc gactcgaaag ccgagcgcat cgacgacgac cggcgccgcc
     8041 agcgctggac cccccgtcgg aagggcagca actggtccgt catcaacatc gccatcgcag
     8101 aggacctcat cgcccagggg cggatgatgc cgagcgggct cgcggccttc gaggcgcgac
     8161 gagaggaccg ctccggcgtc tacacccatg agaacccggc ccaggagctc accgtcgagc
     8221 acgccgccct gctcgcggcc gatccgcgtg cgacagcgtt ctgggaggcg gctccgccgt
     8281 actaccgccg cgtgtgcgtc ggctgggtga acggcgccaa gcagcagacg acccgggacc
     8341 ggcgcatggc gcagctggtg caggattcgg cgaacggcca gctcatcaag agccagcgct
     8401 acggcgacac cccgacctgg gtgacccggg cccgggccgc tgcggacgga gtcgcgtcac
     8461 ccccgctttt tcccgcacca gcgcggtgat caccgcgctg gtgcgggaca gagtcgtgcg
     8521 tgcccacgac gggtacgact cctaccggta cgattcctac catgagggtc accgagaagg
     8581 gtcaggtcac gattccgaag gacatccggg acgccctcgg gatcggggcc ggcaccgagg
     8641 tcgagttcga gcgtcgccgc gacagcatcg tcatccgcaa gagcagccgg ggccgcagcc
     8701 gtgggcagca gctcgccgaa cgtctcaggg gacgagggga cgtcgagatg acgaccgacg
     8761 agatcatggc gctcacccgt ggcgtctgac ggtgtggccg ggaccctcgt cgacagcaac
     8821 gtgctgctgg acatcctgac cgacgacccg aggtgggccg actggtccgc cggcgctctg
     8881 gccgacgcag ccgaggcagg tccgatcttc atcaacccga tcatctactc ggaggtgtcg
     8941 gtgcgcttca cgacggtgga ggcgctcgac gccgccctgc cgtctcggga ctaccggcgc
     9001 gagcccatcc cgtgggcggc ggcgttcctg gcgggcaagg tgttcgtcga ctaccgacgg
     9061 aacacgggca cgaaggcgac gacgctccct gacttcttca tcggcgcgca cgccgcggtc
     9121 gcaggcatgg acctgctgac ccgggacgcc ggtcgctacc ggacgtactt cccgacggtg
     9181 agggtcatcg cacctgactc gtgactgacg ctcgtcaacg aaccccgcac gctttgtccc
     9241 gcaccagcgc ggtgatcacc gcgctggtgc gggacaaagc gtgggggtgg ggtgggacgc
     9301 cgggatgtcc gtcgacggtc ggcggtcagc gagaggcgag gaactcgagg atggagcggt
     9361 tgacccgctc gggctgctcg agctgcagga agtggccggc gccctcgatg acctccaggc
     9421 gcgatccggg cacggtcagc agcggttcgg ccagccggcc gacctcgact ccgatgcagc
     9481 cgtcggactc gccgtggagg tacaggtgcg gctgtgtcgg ggtgccgtcg atcgcggcct
     9541 cctcggcctg gtagtccgct gtggtgtgct ccgcggggtt gagggtggcc cggtagtact
     9601 cgatcgcggc gtgcaggttc gccgggtccc gcagcgactc cttgacccgc tcgacatgct
     9661 cgggagcgtc gagcccgggt gaccagtcct gccacaggtg gtcgatgaac tcgaggtcgt
     9721 tcatggcgac ggcgatctcg gccagcgggt tctggaagaa gaacatgtac caggagcgcc
     9781 ggagctgcgg gtaggtgagg aagccctgcg ccacggccgc cgacggcggc accgacgcgg
     9841 tgaccaatcg actccatcga tccggtgcga gcaccgacgc accggtcgcg atgcgtgcgc
     9901 cccagtcgtg gccgatgagc acggcgtcgg cgtcacctcc gagcgcctcg tgcatcgcca
     9961 ccacgtcggc caccgacgcg ccgatctggt agtggtcgtt ggggtcgagc ccgctcggcg
    10021 cgtagccgcg cagccacggg gcgacggccc ggaacccggc gtcggccagg gccggcagca
    10081 ggtgcgtcca cgtgtgggcc gtgtcgggga agccgtgcag gcacaccgcc agcggtccgc
    10141 tgccaccggc cgcgaggtag gcgaggtcga tgtcgccgac ccggacgtgg tgctcggccg
    10201 gttccgtcgt cgatgtcaat gtcgttcccc cgtgaccgac gcggaccgct cccgcgtca
//
LOCUS       DAOQOD010000109        10160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2388_length_10160_cov_11.622266, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000109 DAOQOD010000000
VERSION     DAOQOD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_10160_cov_11.622266"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..728
                     /locus_tag="PLZ72_10935"
     CDS             162..728
                     /locus_tag="PLZ72_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36855.1"
                     /translation="MSVSTLVLLGLAVVWAIVLLPEVLKRVSKARSGDSIQSFNSQLS
                     SLQRRPAGGSNVVDLRSAQRRAQPGRAGARPTAGPRSAGRPAPVPATVSPAVRRRRQE
                     VLTVLGSASVLTLLCSVAFGGPFLLLFLISAGLLVAYVFALYQVTNAAAASAARDPYG
                     RPSVAPGPRPTDLPGVRISPVSARRIAN"
     gene            757..1386
                     /locus_tag="PLZ72_10940"
     CDS             757..1386
                     /locus_tag="PLZ72_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36856.1"
                     /translation="MTDPADLSARIEQVRADLDAANQARELALPGCRAVIRHCGSSIK
                     AVHRLQFDRAADLADAAEAELRQVQAAVADHPAIEHAGFLHDAEKEYVEARAVRAFVD
                     GVTVPSASELAVGGPAYLRGLAEAASELRRHLLDRLREGDVARAEQLLGLMDDTYDAL
                     STVDYPDAVTHGLRRTLDALRAVLERSRGDLTTSVLQLRLQQAIESDRP"
     gene            1464..2330
                     /locus_tag="PLZ72_10945"
     CDS             1464..2330
                     /locus_tag="PLZ72_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA36857.1"
                     /translation="MEPALDGVEVVLHGGVANVGAVVRVGDHVLRPSNPHTADIHRFL
                     TDLRDAGFDGVPRPVGVDPDGRERLEYVEGDVALPPFPGWFQEPEALASVARLLARFH
                     AASADLDLSGLEWSSEMVDPAVSELPAERAAEGAVPAGVVVCHNDVCPENVVFRNGEA
                     VALLDFDFAAPGRPVHDLSQFVRMCVPVADDEYAESLGFAPGDRAVAVRAVCDAYDDA
                     GGELGPEGRQAMLDLLDRSIARSGEWVRAKAEAGHPGFIEMWASVGGQETYDRRRRWW
                     SEERHRFAAALA"
     gene            2388..2461
                     /locus_tag="PLZ72_10950"
     tRNA            2388..2461
                     /locus_tag="PLZ72_10950"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2422..2424,aa:Ala,seq:cgc)
     gene            complement(2674..6780)
                     /locus_tag="PLZ72_10955"
     CDS             complement(2674..6780)
                     /locus_tag="PLZ72_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA36858.1"
                     /translation="MSAHVEPVTDAMRGESRPEVDRQLLDAPAHPGGADSAPVTGTGG
                     PPPEVPWYDDGSGGGGDGGGRPHPTEMYRSLPTAARVAVWAVGLGVLWLVLGALLPSG
                     MPLGVVLSGAIIGSATGLTAVGLILIWRANRVINFANGAMAGVAGLTAVHLFVSWGWP
                     YWTTLLTAPLLGLVVGALVEVAIIRRFANAPRLVLTVATIGLAQLLGGIELMIPGKVF
                     GASGMVLGAFETPLNSFETEVGAVIINGNHLLTVLVVPVVVAVLAWFLRRSLAGTAIR
                     AASENTDRARLLGIPVRNLTTAVWAIAGGLAALTFVLQAPFQGAAPSAAAGPAILLPA
                     LAAAIVARMESLPKAFVAAVGLGVMQQVVRWNTDTPSLVDVAILIVILVALLVGERSK
                     SRAHDADGGWRNADLVRSLAPAVAALPLVKWSKRVGLVLLIAGAVLLPTQLGPSDAFS
                     VSIMGVWAIVAVSLVVLTGWGGQISLGQFAIVGAGAIVAGNLVTRWNIDLFLVLAIAT
                     LTGALVAVLLGVPALRIKGPFLAVVTLAFAVVLDGYVLNPNVFPELIPQTVTRPLLWG
                     RVDLEDERAMLWFTLAWLAVCILVARSVRRSRSGRLLIAARDNRKATEAASVSARWAT
                     LQGFIFSGALAGLAGGLHVLLLHGAREGSYQVVQSVEVFSGATIGGLGSLGGAIVGAA
                     GLRGAQDLDATIRLVGAGIGVLIVLWAVPAGLAGLAARLRDRIVTPIARRAGMDLEGQ
                     PLAAEAPATAPAPASAPTEPPAVTLPGVAAPAAASAPLPPSAPPPFTGSVPGPLWAPV
                     ADSSQAPQPAPAAAGAPSRATPLLRAQDVDVSYGQLQVLFGVDLDVHEGEVVALLGTN
                     GAGKSTILKAICGLSASSGAISLGDQKISGRSPEQIVRDGIALMPGGKAIFPTLSVTD
                     HLRLSCWTFRKDSARIDEDLAEVHRLFPILEERRHQLAGDLSGGEQQQLALAMTLLLR
                     PRVLLIDELSLGLSPMVVAALCDVVRTLNRDGMTIVVVEQSVNVALTLAERAVFMEKG
                     TVRFEGPTRELLERPDLLRSVFIEGAGGGDESPEDDADEVLAAIDLTGLDTPGSVAAF
                     SGAADAGGARPTVLRCRDVTKRFGGVQALSHVDLTVSAGEIVGLIGQNGAGKTTLMDC
                     ISGFHRIDDDLSSGAIIFRGVDVTAWNPSERARSGMGRTFQEARLFPSLTVTETLAVA
                     CERGVGSRSMVADATRMPASYLAEDDTMTRVHELTRMLGLVRYAHTPTSVLSTGTRRI
                     VELGCLLAEDPVLMLLDEPSAGVAQRETEALGPLLRRIRDHTGAAMLVIEHDMPLLSG
                     LCDRLVAMELGSVLVEGPPAEVLEHPLVVASYLGTDAAAINRSGAAVT"
     gene            complement(6777..8468)
                     /locus_tag="PLZ72_10960"
     CDS             complement(6777..8468)
                     /locus_tag="PLZ72_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36859.1"
                     /translation="MADGARDRLFRGYGPLIGFAAIFLAVAVLVPSQQREVRVEAAGG
                     SGSGSELSSDDASVAGATETVPGSETGLDATGASLDGTGAAGGTAGGGAGGANAKGGS
                     NSGPPKVAGCGAAQIPGDPYSPPCVQFSGGNGGATSRGVTADKIVVSVRVGSFANGML
                     DALSKVANAKIPNESPQVITKTVDGLVEYFNKRFQFYGRKLEVVKWDGKGDVLKETTG
                     GGQEGAQADALKVSQEIKAFADVSAVSPVYADALASKQVINIGVPFTSREWMTQRKPW
                     SWSQFTDCSTVVESVASYYAIKLAGQPAALAGGDLKGQARRAMIIAPENSWYQECVRA
                     GINILQKAGKAGDLVDSLKYQIDIPRMSDQAASLIQKLKAERITTVVCGCDPLLLTFL
                     TSKAKEQNYQPEWVITGIALIDNDMIGQILQQDQWGRAFGVSFSGPTQAAGQGLGYRA
                     YKSVRPDEPSVGVELIYNQLYLLAIGIQGAGPNLNPQTFEKAMFDYPRRSGPSGTWGF
                     GPGDYTTSDDAREVFWNPSVASVQTRSPGTYQDPNGGKRFPIGQWPNTPPRSALG"
     gene            complement(8616..10031)
                     /locus_tag="PLZ72_10965"
     CDS             complement(8616..10031)
                     /locus_tag="PLZ72_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36860.1"
                     /translation="MLRASFLVVAVAAFGSSFGPASGDGFHTGDAQASADTFTLNIKA
                     ANAAIGFTYGRSLAAYQDKTGTSEGRALDLGALPVIFTPDQCDGTPGLLDSKQLPPLT
                     RADSSEEGSNNSRRVQVFTPGVNGEPMGPSTGFQDATATPLPSSAALTESAPADVGLI
                     AIDGGRTRVSTQLKDQVREAHAIMEADQLRVMGGLFTFNQPRWEATTQSGARSSSTGS
                     FTFTSATVLGIPRSAADAMADLEGFKYGLEQLLAPLGVVLELPRVEVRDDGVKVTPMG
                     FRIVNPPFGTQVLIPFLGQIDTQVQQWRKDLVAADCKNATTLTIVDLILGVMGGSGAV
                     EILAGGVDVSTHDTDYSIPPIEPMPEVAAPVDTVPPAPDTFVADYSTDLGDVSSFDTP
                     LDSGTTLDMTTGLGETAVVPSTGGATKEQAALPTAALGGMEEGTAGKAGVAVGILALL
                     GAVGLSVGDRLVGRRSRRVIP"
BASE COUNT         1451 a         3562 c         3745 g         1402 t
ORIGIN      
        1 gggggcgggc cgcggggcgc gtacctgaat cacaggcgtg tagttccgtc aaggacttgt
       61 aacacaagtc gcgcgaccgt taagttctca ggtgagttcg tcgcgatgtc ccccaggcac
      121 ccggcgttcg atcagcagtc gaaaggcctg tccaccctcc catgagcgtc tccacactgg
      181 tcctgctggg tctcgcggtc gtctgggcca tcgtgctcct gcccgaagtg ctcaagcgtg
      241 tctccaaggc ccgcagcggg gacagcatcc agtcgttcaa ctcccagctg tcgtcgctgc
      301 agcgccgacc ggcgggaggc agcaacgtcg tcgacctgcg ctccgctcag cggcgagccc
      361 agcccggacg ggccggcgcc cgccccacgg ccggcccccg cagcgccgga cggccggcgc
      421 cggtccccgc cacggtgtct ccggcggtgc gtcgtcgccg ccaggaggtc ctcacggtcc
      481 tcggctcggc cagcgtcctg accctgctgt gctcggtggc gttcggtgga ccgttcctgc
      541 tgctgttcct gatctcggcc gggctgctgg tggcgtacgt gttcgccctc taccaggtga
      601 ccaacgccgc agcggcgtcc gccgcccgcg acccctacgg acgcccgtcg gtggctcccg
      661 gaccccgccc gaccgacctc cccggcgtgc ggatcagccc ggtgagcgca cgtcgcatcg
      721 ccaactgagt cgaccgcgac ccgggatccg gttcccgtga ccgaccccgc cgacctcagc
      781 gccaggatcg aacaggtccg ggccgacctc gacgccgcca accaggctcg cgagctcgcc
      841 ttgcccggct gtcgggccgt catccgccac tgcggaagct cgatcaaggc cgttcaccgg
      901 ctccagttcg accgggccgc cgatctggcc gacgcggccg aggccgagct gcgccaggtg
      961 caggctgcgg tggccgatca ccccgccatc gaacacgccg ggttcctcca cgacgccgag
     1021 aaggagtacg tcgaagcccg agcggtgcgg gccttcgtcg acggcgtcac ggtcccatcc
     1081 gcgtcggagc tggccgtggg cggaccggcg tacctgcggg gcctggccga agcggccagc
     1141 gagctgcgcc gtcacctgct cgaccgcctg cgcgagggcg acgtcgcccg ggccgagcag
     1201 ctcctcgggc tgatggacga cacctacgac gcgctcagca ccgtcgacta ccccgacgcc
     1261 gtcacccacg ggctgcgccg caccctggat gccctacggg ccgtgctcga acgcagccgc
     1321 ggtgacctga cgacctcggt gctgcagctg cgactccagc aggcgatcga gtccgaccgt
     1381 ccctgaccgg tccgcggccg ctctccctcc ggttcaaggg gatcctcgga tccggcggga
     1441 cacgcagctg cgatactgcc ccgatggagc cagccctgga cggagtcgag gtcgtgctgc
     1501 acggcggggt ggccaacgtc ggcgcggtgg tgcgggtggg cgaccacgtg ctgcgaccat
     1561 ccaacccgca caccgcggac atccaccggt tcctgaccga cctgcgagac gccggcttcg
     1621 acggggtgcc ccgtccggtc ggtgtcgacc ccgacgggcg cgaacgcctc gagtacgtcg
     1681 agggcgacgt ggccctcccg ccgttccccg gctggttcca ggagcctgaa gccctggcgt
     1741 cggtggcccg gctcctggcc cgcttccacg ccgcatcggc ggatctcgac ctgtcgggcc
     1801 tcgagtggag ctccgagatg gtcgacccgg ccgtatccga gctcccggcc gagcgcgcgg
     1861 ccgaaggcgc ggtgccggcc ggagtggtgg tgtgccacaa cgacgtgtgc cccgagaacg
     1921 tggtgttccg gaacggcgag gcggtcgccc tgctcgactt cgacttcgcg gcgcccggtc
     1981 gccccgtcca cgacctgtca cagttcgtgc ggatgtgcgt tcccgtggcc gatgacgagt
     2041 acgccgagtc gctcggcttc gcccccggtg accgggccgt ggcggtgcgg gcggtgtgcg
     2101 acgcctacga cgacgcgggc ggtgaactcg ggccggaggg gcggcaggca atgctggacc
     2161 tcctcgaccg ctcgatcgcc cgcagcggcg agtgggtccg ggccaaggcc gaggccggtc
     2221 accccggttt catcgagatg tgggccagcg tcggcggcca ggagacctac gaccgtcgcc
     2281 gtcgctggtg gtccgaggaa cgccaccggt tcgctgcggc cctcgcctga ggcggcgccc
     2341 gtcgtgtggg agggggtcgg cgggaccgct atgctgtcgg ctcctgcggg ggtgtagctc
     2401 agctggctag agcgcctcgg tcgcatcgag gaggtcgtgg gttcgactcc catcacctcc
     2461 accgcagaag ggccccgacc gctcccggtc ggggcccttc ctcgttcctc cggctcgtgg
     2521 ttccggcggt tccctggatt ttcatgactt ctggcgccac cagtggcgcc agaagtcatg
     2581 aaaacggaga aggcgccgga aacgcggaga gcgccggaaa cgcggagagc gccggaaacg
     2641 cggagagcgc cggaaacgcg gggcccgcgc gggtcaggtg acggcggcgc cggagcggtt
     2701 gatggcggcg gcgtcggtgc ccaggtacga ggcgaccacg agcgggtgct cgagcacctc
     2761 ggcgggcgga ccctcgacca gcaccgatcc gagctccatg gcgaccagac ggtcgcacag
     2821 ccccgacagc agcggcatgt cgtgctcgat gaccagcatc gccgctcccg tgtggtcccg
     2881 gatgcgtcgc agcagcgggc cgagcgcttc ggtctcgcgc tgggcgacac cggcggacgg
     2941 ctcgtcgagc agcatcagca ccgggtcctc ggccagcagg cagccgagct cgacgatgcg
     3001 gcgggtgccg gtcgacagca ccgacgtggg ggtgtgggcg tagcggacca ggccgagcat
     3061 ccgggtcagc tcgtgcaccc gggtcatcgt gtcgtcctcg gccaggtacg acgccggcat
     3121 ccgggtggcg tcggcgacca tcgaccggct gcccacccct cgttcgcacg cgacggcgag
     3181 ggtctcggtc accgtcagcg acgggaacag gcgggcctcc tggaacgtgc ggcccatgcc
     3241 gctgcgggcc cgctccgacg ggttccaggc ggtcacgtcg acgccccgga agatgatcgc
     3301 tccgctgctg aggtcgtcgt cgatgcggtg gaagcccgag atgcagtcca tcagggtggt
     3361 cttgccggcg ccgttctggc cgatcagacc gacgatctca cccgcactga ccgtcaggtc
     3421 gacgtggctg agggcctgca cgccgccgaa gcgcttggtg acgtcgcggc agcgcagcac
     3481 ggtcgggcgg gcgccgccgg catccgcggc accgctgaac gcggcgacgg aaccgggggt
     3541 gtcgaggccg gtcaggtcga tggcggcgag cacctcgtcc gcgtcgtcct cggggctctc
     3601 gtcgccgccg ccggcgccct cgatgaacac cgagcgcagc aggtcgggcc gttcgagcag
     3661 ctcacgggtg gggccctcga agcgcacggt gcccttctcc atgaacacgg cccgttcggc
     3721 cagggtgagc gccacattga ccgactgctc gaccaccacg atggtcatgc cgtcccggtt
     3781 cagggtgcgg accacgtcgc agagggcggc gaccaccatg ggcgacaggc cgagcgacag
     3841 ctcgtcgatc agcaggaccc ggggccgcag cagcagggtc atggccagcg ccagctgctg
     3901 ctgctcgccg ccggagaggt cgccggcgag ctggtggcgg cgctcctcga ggatggggaa
     3961 gaggcggtgc acctcggcca ggtcctcgtc gatgcgggcg gaatccttgc ggaacgtcca
     4021 gcacgacagg cgcaggtggt cggtgacgct gagggtgggg aagatcgcct tgccgccggg
     4081 catcagggcg atgccgtcgc gcacgatctg ctcgggggag cgaccgctga tcttctggtc
     4141 gcccaggctg atcgcccccg agctggccga cagaccgcag atcgccttga ggatcgtcga
     4201 cttgccggcg ccgttggtgc cgaggagggc gacgacctct ccctcgtgga cgtcgaggtc
     4261 gacgccgaag agcacctgga gctggccgta gctgacgtcg acgtcctggg cccgcagcag
     4321 cggggtggcc ctggacgggg cgccggccgc ggccggtgcc ggctgaggtg cctgcgatga
     4381 gtcggcgacc ggcgcccaca acggaccggg gaccgacccg gtgaacgggg gaggggcgct
     4441 cggcggcagc ggagccgatg ctgcggcggg ggcggcgacc ccgggcaggg tgaccgcggg
     4501 cggctcggtc ggagccgagg cgggggccgg ggccgtcgcg ggtgcctcgg cggccagggg
     4561 ctgaccctcc aggtccatgc cggcgcgtcg ggcgatcggg gtgacgatgc ggtcccgcag
     4621 acgggcggcc agccctgcga gaccggcggg gacggcccac agcacgatca gcacgccgat
     4681 gccggcgccg accaggcgga tggtggcgtc gaggtcctgg gcgccgcgca gtcccgctgc
     4741 gcccacgatg gcgccgccga gggagccgag gccgccgatg gtcgcccccg agaacacctc
     4801 gaccgactgc accacctggt agctgccctc ccgggcgccg tgcagcagca gcacgtgcag
     4861 gccaccggcc aggccggcga gcgcaccgga gaagatgaag ccctggaggg tggcccagcg
     4921 ggccgacacc gacgccgcct cggtggcctt gcggttgtcg cgggccgcga tgagcagacg
     4981 accggagcgg gaccgcctca cgctgcgggc caccaggatg cacaccgcca gccacgcgag
     5041 ggtgaaccac agcatggccc gctcgtcctc caggtcgacc cgcccccaca gcaggggtcg
     5101 ggtgacggtc tgggggatca gctcggggaa gacgttcggg ttgaggacgt agccgtccag
     5161 caccaccgcg aacgccaggg tgaccacggc caggaacggg cccttgatcc gcagcgccgg
     5221 gacgccgagc agcaccgcga ccagggcgcc ggtcagcgtg gcgatcgcca ggaccaggaa
     5281 caggtcgatg ttccagcggg tgaccaggtt gccggccacg atcgccccgg cgccgacgat
     5341 ggcgaactgg ccgaggctga tctggcctcc ccagccggtg agcaccacca gcgacaccgc
     5401 cacgatcgcc cacacgccca tgatcgacac cgagaaggcg tcggacgggc cgagctgcgt
     5461 cggaagcagc accgcgccgg cgatgagcag caccagcccg acccgcttgg accacttgac
     5521 cagcggcagc gcggccaccg cgggggcgag cgagcggacc aggtccgcgt tgcgccagcc
     5581 gccgtccgcg tcgtgggccc gcgacttgct gcgctcgccg accagcagcg ccaccaggat
     5641 gacgatcagg atcgccacgt cgaccagcga cggcgtgtcg gtgttccagc ggacgacctg
     5701 ctgcatgaca ccgaggccga ccgcggcgac gaacgccttg ggcagcgact ccatgcgggc
     5761 gacgatggcg gcggcgagcg ccggcagcag gatggccggc ccggccgccg ccgagggcgc
     5821 cgcaccctgg aacggggcct gcagaacgaa ggtcagagcg gcgagaccgc cggcgatcgc
     5881 ccacaccgcg gtggtcaggt tgcgcaccgg gatgccgagc agacgggccc ggtcggtgtt
     5941 ctccgacgcg gcccggatgg cggtgccggc cagcgaacgt cgcaggaacc aggccaggac
     6001 ggcgacgacg acgggcacca ccaggacggt gagcaggtgg ttgccgttga tgatgacggc
     6061 gccgacctcg gtctcgaagg agttgagcgg cgtctcgaac gcgccgagca ccatgccgga
     6121 cgcgccgaag accttcccgg ggatcatcag ctcgattccg ccgagcagct gggccagacc
     6181 gatcgtggcg acggtgagga ccaggcgggg ggcgttggcg aaccggcgga tgatggcgac
     6241 ctcgaccagg gcgccgacga ccaggccgag cagcggagcg gtcagaaggg tggtccagta
     6301 cggccagccc cacgacacga acaggtgcac cgcggtcaga ccggcgacgc cggccatggc
     6361 gccgttggcg aagttgatga cccggttggc ccgccagatc aggatgaggc cgacggcggt
     6421 gaggccggtg gccgagccga tgatggcgcc ggagagcacg acgccgagcg gcatccccga
     6481 cggcaggagc gcgccgagaa cgagccacag cacgccgagg ccgacggccc acaccgccac
     6541 ccgggcagcg gtcggcagcg accggtacat ctcggtgggg tgggggcggc cgcccccgtc
     6601 gccgcccccg cccgagccgt cgtcgtacca cgggacctcg ggcggcgggc cgccggtgcc
     6661 ggtcaccggt gcggagtcgg cgccgccggg atgtgcgggg gcgtcgagca gctggcggtc
     6721 gacctcgggt cgggactcgc ctcgcatggc gtcggtcacc ggctcgacgt gggcgctcat
     6781 ccgagagccg atcggggcgg ggtgttgggc cactggccga tcgggaagcg cttgcccccg
     6841 ttgggatcct ggtaggtgcc gggggagcgg gtctgcaccg aggccaccga cgggttccag
     6901 aacacctcac gggcgtcgtc ggaggtcgtg tagtcacccg gcccgaagcc ccacgtcccc
     6961 gacggtccgg agcggcgggg gtagtcgaac atggccttct cgaaggtctg cgggttcagg
     7021 ttggggcccg cgccctggat gccgatggcc agcaggtaca gctggttgta gatcagctcg
     7081 acgccgaccg acggctcgtc gggccgcacc gacttgtagg cccggtagcc gagaccctga
     7141 ccggcggcct gggtcggtcc cgagaagctg acgccgaacg cccggcccca ctggtcctgc
     7201 tggaggatct gcccgatcat gtcgttgtcg atcagggcga tgccggtgat cacccactcg
     7261 ggctggtagt tctgctcctt ggccttcgag gtcaggaacg tgagcagcag cgggtcgcat
     7321 ccgcacacga cggtggtgat gcgctctgcc ttgagcttct ggatgagcga ggcggcctgg
     7381 tccgacatgc gggggatgtc gatctggtac ttcagcgagt cgaccaggtc gccggccttg
     7441 ccggccttct gcaggatgtt gatgccggcc cgcacgcact cctggtacca ggagttctcg
     7501 ggggcgatga tcatcgcccg gcgggcctgg cccttcaggt cgccgccggc gagcgcggcg
     7561 ggttgaccgg cgagcttgat cgcgtagtag ctggcgacgc tctccaccac ggtcgagcag
     7621 tcggtgaact ggctccagga ccagggcttg cgctgggtca tccactcccg ggaggtgaac
     7681 ggcaccccga tgttgatcac ctgcttggag gcgagggcat cggcgtacac cggcgacacc
     7741 gccgagacgt ccgcgaacgc cttgatctcc tgggagacct tgagggcgtc ggcctgggcg
     7801 ccctcctggc cgccgccggt cgtctccttc aggacgtcgc ccttgccgtc ccacttcacg
     7861 acctcgagct tgcggccgta gaactggaag cgcttgttga agtactcgac gaggccgtcg
     7921 acggtcttgg tgatgacctg ggggctctcg ttggggatct tggcgttggc gaccttggac
     7981 agggcgtcga gcatgccgtt ggcgaagctg cccacccgga ccgacaccac gatcttgtcg
     8041 gcggtgacgc ccctgctggt cgcccctccg ttgccgccgg agaactgcac gcacggcggg
     8101 gagtacgggt cgccggggat ctgggcggca ccgcagcccg ccaccttggg cggaccggag
     8161 ttggaaccgc ccttggcgtt cgctccgccc gctccgccgc cggcggtgcc gccggcggcg
     8221 ccggtgccgt cgagggaagc gccggttgcg tcgaggcccg tctcggatcc ggggacggtc
     8281 tcggtggcgc cggccacgct ggcgtcgtcg gagctgagct ccgatccgct gccggaacct
     8341 cctgccgcct ccacccgcac ctcgcgctgc tggctgggca ccagcaccgc caccgccagg
     8401 aagatcgcgg cgaacccgat cagcgggccg tagcccctga acaacctgtc gcgtgctccg
     8461 tcagccatct ccgctctccg ctctcgatgt ctccacttgg accgacaccg ccgcgtcctg
     8521 cggtgtcggt cgtcgatctg gtgagttcgg acgggcgcat ccggtgctcc cgtcgttctg
     8581 gccgctccgg cgtcggaccg aaccccgggg tcctgtcagg ggatgacccg ccgggagcgt
     8641 cgtccgacca gccggtcccc gaccgacagc ccgaccgcac ccagcagcgc gaggatcccc
     8701 acggcgaccc ccgccttgcc ggccgttccc tcttccatgc cgccgagcgc cgcggtgggc
     8761 agggccgcct gctccttggt ggctccgccc gtggacggca cgacagcggt ctcgcccagc
     8821 ccggtggtca tgtcgagcgt ggtcccgctg tcgagcgggg tgtcgaacga cgacacgtca
     8881 ccgaggtcgg tgctgtagtc cgccacgaac gtgtcgggcg cgggagggac cgtgtcgacc
     8941 ggagccgcca cctcgggcat cggttcgatc ggcgggatcg agtagtcggt gtcgtgggtg
     9001 gagacgtcga cgccaccggc gaggatctcg accgcaccgg agccgcccat gaccccgagg
     9061 atgaggtcga cgatcgtcag cgtggtcgcg ttcttgcagt ccgccgcgac gaggtccttg
     9121 cgccactgct ggacctgggt gtcgatctgg ccgaggaacg ggatcagcac ctgtgtcccg
     9181 aagggcgggt tcacgatccg gaaccccatc ggggtgacct tcacgccgtc gtctcggacc
     9241 tcgacccggg gcagctccag cacgacgccg agcggagcga ggagctgctc gagcccgtac
     9301 ttgaaaccct cgagatcggc catggcgtcc gccgcggacc gggggatgcc gagcacggtg
     9361 gcgctggtga aggtgaacga accggtcgag gagctgcggg cgccggactg ggtggtggcc
     9421 tcccagcggg gctggttgaa ggtgaacagg ccgcccatca cccggagctg gtcggcttcc
     9481 atgatcgcgt gcgcttcacg gacctggtcc ttgagctggg tcgagacccg ggtgcggccg
     9541 ccgtcgatgg cgatgaggcc gacgtccgca ggcgccgact cggtcagggc cgccgaggac
     9601 ggcaacgggg ttgccgtcgc gtcctggaag ccggtcgagg ggcccatcgg ctcgccgttg
     9661 acgccggggg tgaagacctg gacccgacgc gagttgttgg agccctcctc cgacgagtcc
     9721 gcccgggtga gcggggggag ctgcttggag tcgaggagtc cgggggtgcc gtcgcactgg
     9781 tcgggggtga agatgaccgg tagggcgccg aggtccaggg cccgaccctc ggaggtgccg
     9841 gtcttgtcct ggtaggcggc gagcgaccgt ccgtaggtga agccgatcgc ggcgttggcc
     9901 gccttgatgt tgagcgtgaa cgtgtcggcg ctggcctggg cgtcaccggt gtggaacccg
     9961 tcgccggacg cggggccgaa cgacgacccg aacgcggcga ccgccaccac caggaacgag
    10021 gcacgcagca ccgatcgtcc ggacggcgac cgacgggcgg agcccgcgcg ccgactgcgt
    10081 gacccgaccc gatccgatcc ccggacctct ccatccacca cgctgtcccc cgacatcgtt
    10141 gcgctcgccg acgccgacct
//
LOCUS       DAOQOD010000110        10123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2399_length_10123_cov_10.535360, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000110 DAOQOD010000000
VERSION     DAOQOD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2399_length_10123_cov_10.535360"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLZ72_10970"
     CDS             <1..255
                     /locus_tag="PLZ72_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36861.1"
                     /translation="EPELAAACTAAMLSNDPDVKVLRDRIGLTWRLRLQAALGDDADP
                     AVLRSLEFAVSGAMLQAGMGHLDYTDLPDQLARTAELVVG"
     gene            279..1505
                     /locus_tag="PLZ72_10975"
     CDS             279..1505
                     /locus_tag="PLZ72_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36862.1"
                     /translation="MSTAIDDAIAFDPWAYEFHEDPYPLYERLREEAPLYRNDELGFW
                     ALSRHEDVIEGFRDFERLSSSHGVSLDPAASGPNAHRTMSFLALDPPMHGRMRGLVSK
                     GFTPRRVAEQEPHIRDLARRYVERMRDTPDDLDFISDFAGRMPMDVISELMGVPESDR
                     EELRRLSDLLVHREEGIRDVPPEGAAAALDLVVYYSDMLAQRRARPTDDLTSALLAVE
                     IDGDRLTDEEIIGVLFLLVVAGNETTTKLLANAWYWAWRFPDQRAKPFADPARIPQWI
                     EETLRFDTSSQMLARTATVDIPAHGGTIPAGDRVLLLVGSANRDPRAFTAPDVYDLDR
                     PEPLPQLASFGFGRHYCMGASLARLEARVALEELLSAFSDYDIDPDGLERVHSVNVRG
                     FSKVPTSVTLRSGGNR"
     gene            1505..2323
                     /locus_tag="PLZ72_10980"
     CDS             1505..2323
                     /locus_tag="PLZ72_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36863.1"
                     /translation="MPRFEPHPDRRTAVVTGASSGHGAAIAARLAAAGHPVVVGARRV
                     DRVEELAAKIAADGGEAVAVALDLTDPDSIADFARRAEDELGPIEIVVSNAGDVQPIT
                     TLADPVEFHRQMQVNLLGAQALVHEIVPAMVARRRGDVVFVTSEVAVDPRTHMAAYVA
                     SKAGLEGFARAMAMECEGTGVRVGIVRPGPSSTEQGTTWSVDSINEVVASWSHWGLLR
                     HDGAIRPVEFADAVLAVVSAPKGTRYALLEVQPEAPIGDPAVLRAPSPDDQGEP"
     gene            2320..3747
                     /locus_tag="PLZ72_10985"
     CDS             2320..3747
                     /locus_tag="PLZ72_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36864.1"
                     /translation="MTDTTTAPTGRSADIPKVSGDDGGTGHLEELRVDPIGLMQRVRD
                     ECGDVGEFRLADNDVVLLSGAEANEVFFRASDDELDQAAAYPFMTPIFGEGVVFDATP
                     EERRRALHNQSLRDKFMRGHAATITAEIERMLAEWDDEGEIDLLDWTAELFIYTSTSC
                     LIGPRFREELDVSVAHLFHELERGTDAIAFVDPYADIDSFRRRDAARAALVERVQGIM
                     ARRRAAGPPAEGEERDLVDVLMSIKDPDGNEHFSADVITGMFISMMFAGHHTTSTTAS
                     WFIIEMLRNPDVMAAVVAEMDALADAGGGEITYQDLREVPWLEASIKETLRLHPPLIL
                     LLRLAKVPVEVEGYTIPAGKLVGASPAVSNRLPEAFDDPDRFDPSRYVEPRQDDLDNP
                     WNWIPFGAGRHRCVGAAFAMMQLKTIFCILLRDWELTAAQPPETYRNDHSKMVVQLAQ
                     PCMVRYRRRSGPTVVEDGSPTGESA"
     gene            3747..3950
                     /locus_tag="PLZ72_10990"
     CDS             3747..3950
                     /locus_tag="PLZ72_10990"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNA36865.1"
                     /translation="MAFRIVVDSDLCQGHGVCENEAPELFEVGRDMLAHVLVEQADES
                     QRRAAEAAAKYCPTHAITLVPVL"
     gene            4023..4577
                     /locus_tag="PLZ72_10995"
     CDS             4023..4577
                     /locus_tag="PLZ72_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA36866.1"
                     /translation="MPGFPREELEEMVQRWLQANRDAEAAGDWRPLAELYTPDATYGW
                     NYGPDTDFMAVGRDEIRDLALGAEMGGLDGWQYPYEEILIDDRKGFVVGFWRQVADAT
                     RPDGTPYEVNGIGGSWFRYAGDFQWDWQRDWFDFGNAARTFLDMMSAGVLSEGMTARM
                     NRSMRGPLPGYYPAGTSPVKIWER"
     gene            4589..6058
                     /locus_tag="PLZ72_11000"
     CDS             4589..6058
                     /locus_tag="PLZ72_11000"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNA36867.1"
                     /translation="MLADAGLDVVLVDGTLRPATGGGTFETSNPATEEVLGVAADGTA
                     ADMDAAIAAARAAFDAGTWAGDAALRARCLRQLRDALVAHGEEMRDLTTAEVGAPAFL
                     TAAAQYDFPVEDLSYWAELAEGYDGWVTDLGEKESFGIVSHRRVHREPAGVVGAITPW
                     NFPHQINFAKVGPALAAGCTAVLKPAPDTPWCASALARIVAEETDIPAGVLNVVTSTD
                     HSLGSQLARDPRVDVVSFTGSTATGRSVMRDAAENLTRVFLELGGKSAFVVLDDADAA
                     AAAGLAAFTAATHAGQGCAITTRLVVPRSMHDEIVEAAAATMGALTAGDPTDPGTICG
                     PLISARQRDRVQGYLDLAVAEGGTFAVGGGRSPRFDRGYFIDPTLVVGLDNSARVARE
                     EIFGPVLVVIAHDGDDDAVAIANDSPYGLSGAVYSGDEDHALAVAARIRTGTLGINGG
                     IWYGADVPFGGYKQSGIGREMGVAGFEEYLETKAIAVAR"
     gene            6168..6935
                     /locus_tag="PLZ72_11005"
     CDS             6168..6935
                     /locus_tag="PLZ72_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA36868.1"
                     /translation="MASTPNLRFQDRVSIVTGAAGGIGEAYARRLASEGASVVVADID
                     AERAAAVARSIVADGGTALGVAVDISDPDSTREMAAATMDAFGRIDHLVNNAAIFGGM
                     EIDLLLTVDWDYYRRFMSVNMDGALLCIRACWEHMAAGGGGAIVNQSSTAAYMYANYY
                     GLAKAGINGLTIQLAHELGGSNIRINAIAPGPTDTDAARSVVPGSIMDDIVKGLALKR
                     RGTPEDMAGMVSFLLSDDASWITGQIFNVDGGQVVRP"
     gene            6932..7783
                     /locus_tag="PLZ72_11010"
     CDS             6932..7783
                     /locus_tag="PLZ72_11010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36869.1"
                     /translation="MIGFIGLGNIGAPMARRLLDRPDELVVLDVAAAATAPFAAEGAT
                     VAATPAELAARARVISIVVRDETQVAEVLDGPDGLLAAATAGTVVAVHSTISAEGAVA
                     LAERASAAGVDLVDAPISGGAMGAHEGTLAVMVGGSDDAVERARPVLERYASLVVHCG
                     PVGQGTRMKIARNLITFASFAAVGEAQRLAQAAGLDVAALGDVVRHSDRVTGGAGAIM
                     LRPDASVMADDDGLRPIFAHTAALGTKDLELAAELGDGLDVDTPVAELARRLLAPALG
                     LESYPEP"
     gene            7785..9047
                     /locus_tag="PLZ72_11015"
     CDS             7785..9047
                     /locus_tag="PLZ72_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36870.1"
                     /translation="MTAPRTLAEFTHDELARLVREYLLAGQLMDRAGMPHLIGAYGRD
                     EMTAIAIDEWMGASPVYTRRMQQLLGFGRGDVETIFKGMQFDIGAPPEFMDFRYRVTD
                     ANHGEFWLDHCGALMDVEPMGEDYVHAMCHTIEDPTFDATAIATNRRAQVRPIHRPPR
                     EPSDRQPHCHWTVTIDDAHPELPLPEVMAPVQATRLAALELGPIGRGATDAEVADGWN
                     DYTAPLDPDLVTERFSTAALRVLAEEVCVQQHLLVMSFGAAIERRHGTEDAVAVVDRQ
                     FAGIAGLTSQRLRDAFGAGDGSRSGTTLADVAQVLDLHPAFRPRAYVDWRVELADDRL
                     ELTLGDCPARHERGMETWISRLADGHDDGLAAAVEGVTLYARLERTGPSRWSVTLADE
                     PAPDRSEVALTRFSTGATFVFIRYRGAQ"
     gene            9092..>10123
                     /locus_tag="PLZ72_11020"
     CDS             9092..>10123
                     /locus_tag="PLZ72_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNA36871.1"
                     /translation="MRAFVGPTGLLVEGATSGPLAGTTLAVKDVIDVAGRVTGAGNPD
                     LAASRAPAGRSAPVVEALVAAGATVVGKTVTDELAYSLGGDNEHFPPPVNPAAPDRTT
                     GGSSSGSASAVAAGVVDLALGTDTGGSIRVPASYCGILGWRPTHGAVSAEGVTPLASS
                     FDTIGLFARSAPLLRAAAGVALATVAGRPVSPSPVERFAIVAELMDLIGPPGTDVRDA
                     LEGAVLDRFGAAVPTIRLGCDMDECLATFRTLQGAEAWATHGGWIRSSRPRMGENTAY
                     RFDAASKVTAEQVAAARPVRERVRRAVHDATVGGTVLLAPAAAGPAPVRGEYGPEAER
                     IRADTLRSTV"
BASE COUNT         1451 a         3795 c         3505 g         1372 t
ORIGIN      
        1 gaacccgagc tggccgcggc gtgcaccgcg gccatgctct ccaacgaccc cgacgtcaag
       61 gtcctgcggg accgcatcgg gctcacctgg cggctccggc tccaggccgc gctcggcgac
      121 gacgccgatc cggccgtgct gcggtcgctc gagttcgccg tgtcgggggc catgttgcag
      181 gccggcatgg gacatctcga ctacaccgac ctacccgacc agctcgcccg caccgccgag
      241 ttggtcgtcg gctgacacct gaccaacccg gggggaccgt gagcaccgcc atcgacgacg
      301 ccatcgcctt cgacccgtgg gcctacgagt tccacgagga cccgtacccc ctctacgaac
      361 ggctccgcga agaggcgccg ctctaccgca acgacgagct cggcttctgg gcgctgtcgc
      421 gccacgagga cgtcatcgag ggcttccggg acttcgagcg gctgtcgagc tcgcacggcg
      481 tgtcgctcga cccggcggcg tcggggccca acgcgcatcg caccatgtcg ttcctggcgc
      541 tcgacccgcc gatgcacggc cgcatgcgtg ggctggtctc caaggggttc accccgcggc
      601 gcgtcgccga gcaggaaccc cacatccgcg acctcgcccg ccgctacgtc gagcgcatgc
      661 gcgacacccc cgacgacctc gacttcatct ccgacttcgc cgggcgcatg ccgatggacg
      721 tcatctccga gctgatgggc gtacccgagt ccgaccgcga ggagctccgc cgcctgtcgg
      781 acctgctcgt gcaccgcgag gagggcatcc gcgacgtccc gcccgagggg gcggctgccg
      841 ccctggatct ggtcgtctac tactccgaca tgctcgccca gcgccgggcg cgtcccaccg
      901 acgacctcac ctccgcactg ctcgcggtcg agatcgacgg cgaccgcctg accgacgagg
      961 agatcatcgg cgtcctgttc ctgctcgtcg tcgccggcaa cgagaccacg accaagctgc
     1021 tcgccaacgc ctggtactgg gcgtggcggt tccccgacca gcgcgccaag ccgttcgccg
     1081 acccggctcg catcccgcag tggatcgaag agacgctgcg cttcgacacc tcgtcgcaga
     1141 tgctggcgcg gaccgccacg gtcgacatcc ctgcccacgg cggcacgatc ccggcgggcg
     1201 accgggtgct gctgctggtc ggatcggcca accgcgaccc ccgggcgttc accgcccccg
     1261 acgtctacga cctcgaccgt cccgaaccgc tgccgcagct cgccagcttc ggcttcggcc
     1321 gccactactg catgggggcg tcgctcgccc gcctcgaggc ccgggtcgcg ctcgaggagc
     1381 tgctctctgc gttctccgac tacgacatcg accccgacgg cctggaacgg gtccactcgg
     1441 tcaacgtgcg gggcttctcc aaggtgccga cctcggtcac gttgcgttcc ggggggaacc
     1501 gctgatgccc cgcttcgagc cgcaccccga ccgtcggacc gccgtggtga ccggtgcgtc
     1561 gtccggtcat ggcgccgcca tcgccgcccg actggccgcc gccgggcacc ccgtcgtggt
     1621 gggcgcccgt cgggtcgacc gcgtcgagga gctcgcggcc aagatcgccg ccgacggcgg
     1681 cgaggccgtg gccgtcgccc tcgacctgac cgatcccgac tccatcgccg acttcgcccg
     1741 acgggccgag gacgagctcg gaccgatcga gatcgtcgtg tccaacgccg gggacgtgca
     1801 gccgatcacc acgctggccg accccgtcga gttccaccgc cagatgcagg tcaacctgct
     1861 cggcgcccag gccctggtcc acgagatcgt gccggcgatg gtcgcccgcc gacgcggcga
     1921 cgtcgtgttc gtcacctccg aggtggcggt cgatccccga acccacatgg cggcctacgt
     1981 ggcgtccaag gccgggctcg agggcttcgc ccgggcgatg gcgatggagt gcgagggcac
     2041 cggcgtgcgc gtcggcatcg tccgccccgg tccgtcgagc accgagcagg gcaccacctg
     2101 gtcggtcgac tcgatcaacg aggtcgtcgc ctcgtggtcg cactggggcc tgctgcgcca
     2161 cgacggcgcc atccggcccg tcgagttcgc cgacgccgtg ctggcggtgg tgtccgcgcc
     2221 caagggcacc cgctacgccc tgctcgaggt gcagcccgag gcgccgatcg gcgacccggc
     2281 ggtgctgcga gctccgagtc cggacgacca gggggaacca tgaccgacac cacgaccgcc
     2341 ccgaccgggc gctccgccga catccccaag gtgtcgggcg acgacggcgg gaccgggcac
     2401 ctcgaggagc tgcgggtcga cccgatcgga ctgatgcagc gggtgcgcga cgagtgcggc
     2461 gacgtcggcg agttccggct ggccgacaac gacgtcgtgc tgctgtcggg cgccgaggcc
     2521 aacgaggtgt tcttccgggc ctccgacgac gagctcgacc aggcggccgc ctacccgttc
     2581 atgacgccga tcttcggcga gggcgtggtg ttcgacgcca cccccgagga acgccggcgg
     2641 gcgctgcaca accagtcgct gcgcgacaag ttcatgcggg gccacgccgc gacgatcacc
     2701 gccgagatcg agcggatgct cgcggagtgg gacgacgagg gcgagatcga cctgctcgac
     2761 tggaccgccg agctgttcat ctacacctcg acgtcgtgtc tgatcgggcc gcggttccgc
     2821 gaggagctcg acgtgtcggt ggctcacctg ttccacgagc tcgagcgcgg cacagacgcc
     2881 atcgccttcg tcgacccgta cgccgacatc gacagcttcc gtcgccgcga cgcggcccgc
     2941 gccgcgctgg tcgagcgggt ccagggcatc atggcgcgcc gccgggccgc cgggcctccc
     3001 gccgagggcg aggagcgtga cctggtcgac gtgctgatgt cgatcaagga ccccgacggc
     3061 aacgagcact tctccgcgga tgtgatcacc ggcatgttca tctcgatgat gttcgccggg
     3121 caccacacca cctccaccac cgcgtcgtgg ttcatcatcg agatgctgcg caatcccgac
     3181 gtcatggctg ccgtcgtggc cgagatggac gccctggccg acgccggcgg cggcgagatc
     3241 acctaccagg acctgcgcga ggtgccgtgg ctggaggcgt cgatcaagga gacgttgcgc
     3301 ctgcacccac cgctgatcct gctgctgcgc ctggcgaagg tgcccgtaga ggtcgagggc
     3361 tacacgatcc cggccggcaa gctggtcggc gcgtcgcccg cggtgtcgaa ccgactcccc
     3421 gaggcgttcg acgacccgga ccgcttcgac ccgtcgcgct acgtcgagcc ccgtcaggac
     3481 gatctcgaca acccgtggaa ctggatcccg ttcggtgccg gccggcaccg ttgcgtcggc
     3541 gccgcgttcg ccatgatgca gctcaagacg atcttttgca tcctgctgcg cgactgggag
     3601 ctcaccgcgg cgcaaccgcc ggagacgtac cgcaacgacc actcgaagat ggtcgtgcag
     3661 ctcgcccagc cctgcatggt ccgctaccga cgccggagcg gaccgacggt cgtcgaggac
     3721 ggctcgccga caggggagag cgcctgatgg cattccggat cgtcgtcgac tccgacctct
     3781 gccagggcca cggggtgtgc gagaacgagg cccccgagct gttcgaggtc ggccgggaca
     3841 tgctcgccca cgtgctcgtc gagcaggccg atgagtccca gcgccgcgcc gctgaggccg
     3901 cggccaagta ctgccccacc cacgccatca ccctcgtccc cgttctgtag tgcgagatcc
     3961 aggcgaacgc ctggatctcg cactacagaa cggcggggga acgacccaga aggagtagcg
     4021 acgtgcccgg attcccacgt gaggagctcg aggagatggt gcagcggtgg ctccaggcca
     4081 accgcgacgc cgaggccgcc ggagactggc ggccgctggc cgagctctac accccggatg
     4141 ccacctacgg ctggaactac ggccccgaca ccgacttcat ggccgtcggc cgcgacgaga
     4201 tccgcgacct ggccctcggc gccgagatgg gcggcctcga cggctggcag tacccctacg
     4261 aggagatcct catcgacgac cgcaaggggt tcgtcgtcgg cttctggcgc caggtcgccg
     4321 acgccacccg gcccgacggc accccctacg aggtcaacgg catcggcggc agctggttcc
     4381 gctacgccgg ggacttccag tgggactggc agcgggactg gttcgacttc ggcaacgccg
     4441 cccggacctt cctcgacatg atgtcggcgg gggtgctctc cgagggcatg acggcgcgca
     4501 tgaaccgctc gatgcggggc ccgcttcccg gctactaccc ggcgggcacg tcgccggtga
     4561 agatctggga gcgctgagcg tggccgacat gctcgccgac gccggactcg acgtcgttct
     4621 ggtcgacggg acgttgcgcc ccgcgaccgg cggcggcacc ttcgagacgt ccaaccccgc
     4681 caccgaggag gtgctcggcg tcgcagcgga cggcaccgcg gccgacatgg acgcggcgat
     4741 cgccgcggcc cgcgccgcgt tcgacgccgg gacctgggcg ggcgacgccg cgctgcgggc
     4801 ccggtgtctg cgccagctgc gcgacgccct ggtcgcccac ggcgaggaga tgcgggacct
     4861 gaccaccgcc gaggtgggcg ccccggcgtt cctgaccgcg gcggcgcagt acgacttccc
     4921 ggtcgaggac ctcagctact gggcggagct ggccgagggc tacgacggct gggtcaccga
     4981 cctgggcgag aaggagtcgt tcggcatcgt gtcgcaccgc cgagtgcacc gagagccggc
     5041 cggggtggtc ggcgcgatca ccccgtggaa cttcccgcac cagatcaact tcgccaaggt
     5101 cggcccggct ctggccgccg gctgcaccgc ggtgctcaag cccgcccccg acaccccgtg
     5161 gtgcgccagc gccctggccc gaatcgtggc cgaggagacc gacatccccg ccggggtgct
     5221 caacgtcgtc acctcgaccg accactcgct cggctcacag ctggcccggg acccccgcgt
     5281 cgacgtcgtg tcgttcaccg gctcgaccgc gaccggacga tcggtcatgc gcgacgcggc
     5341 cgagaacctg acccgggtgt tcctggagct cggcggcaag tcggccttcg tcgtcctcga
     5401 cgacgcggac gcggccgcgg ccgccgggct ggccgcgttc accgctgcga cccacgccgg
     5461 tcagggatgt gccatcacca cccgcctggt cgtcccccga tcgatgcatg acgagatcgt
     5521 cgaggcagcc gcggccacga tgggcgccct caccgcgggc gacccgaccg acccgggcac
     5581 gatctgcgga ccgctgatct cggcccgcca gcgcgaccgg gtgcagggct acctcgacct
     5641 ggccgtcgcc gagggcggga ccttcgccgt cggtgggggc cgctccccgc gcttcgaccg
     5701 cgggtacttc atcgacccga cgctggtcgt cggcctcgac aactcggccc gggtcgcccg
     5761 cgaggagatc ttcgggccgg tgctggtcgt catcgcccac gacggagacg acgacgcggt
     5821 cgcgatcgcc aacgactcgc cctacggcct gtccggcgcg gtgtactccg gcgacgagga
     5881 ccacgccctg gccgtcgcgg cccgcatccg caccgggacc ctcggaatca acggcggcat
     5941 ctggtacggc gcggacgtcc ccttcggcgg ctacaagcag tccggcatcg gacgcgagat
     6001 gggcgtggcc ggattcgagg agtacctcga gaccaaggcg atcgccgtcg cccgctaggt
     6061 acggcgcgga acgctccggg caccgacgcg gcccgggcca gcacacacac gacagacccg
     6121 acacgacaca cccgacacga cacaccgaca cgacaggaga ccgacccgtg gccagcaccc
     6181 cgaacctccg cttccaggac agggtcagca tcgtcaccgg cgccgccggc ggcatcggcg
     6241 aggcctacgc acgccgcctc gcgtccgaag gggcgtcggt cgtcgtggcc gacatcgacg
     6301 ccgagcgggc cgcggctgtc gccaggtcga tcgtcgcgga cggcggcacc gcgctcgggg
     6361 tggcggtcga catctccgat cccgactcca cccgggagat ggccgcggcg accatggacg
     6421 ccttcggccg catcgaccac ctggtcaaca acgccgccat cttcgggggc atggagatcg
     6481 acctgctgct gaccgtcgac tgggactact accggcgctt catgagcgtg aacatggacg
     6541 gggcgctgct gtgcatccgg gcgtgctggg agcacatggc cgccggcggc gggggagcga
     6601 tcgtcaacca gagctcgacc gcggcctaca tgtacgccaa ctactacggg ctggccaagg
     6661 ccggcatcaa cggactgacc atccagctgg cccacgagct cggcggctcc aacatccgca
     6721 tcaacgccat cgccccgggc ccgaccgaca ccgacgcggc gcgcagcgtc gtgcccggct
     6781 cgatcatgga cgacatcgtc aagggcctgg ccctcaagcg ccgcggcacc ccggaggaca
     6841 tggccggcat ggtcagcttc ctgctctccg acgacgccag ctggatcacc gggcagatct
     6901 tcaacgtcga cggcggacag gtcgtgcgtc cgtgatcggc ttcatcggcc tgggcaacat
     6961 cggcgcgccg atggcgcgac gcctgctcga ccgacccgac gagctcgtcg tgctcgacgt
     7021 cgccgcagcc gccaccgctc cgttcgcggc cgagggcgcg acggtcgccg ccaccccggc
     7081 cgagctggcg gcccgagcgc gggtcatcag catcgtggtg cgcgacgaga cccaggtcgc
     7141 cgaggtgctc gacggacccg acggcctgct cgccgcagcg acggccggca ccgtggtggc
     7201 agtgcactcg accatcagcg ccgagggcgc cgtcgccctc gcagaacggg catccgccgc
     7261 gggggtcgac ctggtcgacg ccccgatcag cggcggggcg atgggcgccc acgagggcac
     7321 cctggcggtc atggtcggcg ggtccgacga cgccgtcgaa cgggcccgcc ccgtcctcga
     7381 gcgctacgcg tcgctggtcg tgcactgcgg tccggtcgga cagggcaccc ggatgaagat
     7441 cgcccgcaac ctgatcacct tcgcctcctt cgccgccgtt ggcgaggcgc agcgactggc
     7501 gcaggccgcc gggctcgacg tggccgccct gggcgacgtc gtgcgccact cggaccgggt
     7561 caccggcgga gcgggagcca tcatgttgcg cccggacgcc tcggtgatgg ccgacgacga
     7621 cggactgcgt ccgatcttcg cccacaccgc cgcgctcggg accaaggacc tggagctggc
     7681 tgcggagttg ggcgacggac tcgacgtcga cacccccgtc gccgagctgg cccggcggct
     7741 gctcgccccg gcgctgggcc tcgagtcgta cccggagccc tgaggtgacg gcgccgcgga
     7801 cgctggccga attcacccac gacgagctgg cccgcctggt ccgggagtac ctgctcgccg
     7861 gccagctgat ggaccgggcc ggcatgccgc acctgatcgg cgcctacggc cgcgacgaga
     7921 tgaccgccat cgccatcgac gagtggatgg gggcgagccc cgtctacacc cggcgcatgc
     7981 agcagctgct gggcttcggg cgcggcgacg tcgagacgat cttcaagggc atgcagttcg
     8041 acatcggcgc cccgccggag ttcatggact tccgctaccg ggtgaccgac gcgaaccacg
     8101 gcgagttctg gctggaccac tgcggcgccc tcatggacgt cgaaccgatg ggcgaggact
     8161 acgtccacgc gatgtgccac acgatcgagg acccgacctt cgacgccacc gccatcgcca
     8221 ccaaccgccg ggcccaggtc cgcccgatcc accgtccgcc ccgcgagccc tccgaccgcc
     8281 agccccactg ccactggacg gtgacgatcg acgacgccca ccccgaactg ccgctgcccg
     8341 aggtgatggc gccggtgcag gccacccgcc tggccgcgct cgagctcggc ccgatcgggc
     8401 gcggggccac cgacgccgag gtcgccgacg gctggaacga ctacaccgct ccgctcgacc
     8461 ccgatctggt caccgagcgg ttctcgaccg ctgctctgcg ggtgctggcc gaggaggtct
     8521 gcgtccagca gcacctgctg gtcatgtcgt tcggcgccgc gatcgaacga cgtcacggca
     8581 ccgaggacgc cgtggcggtc gtcgaccggc agtttgccgg catcgccggg ctgacctcgc
     8641 aacggctccg cgacgcgttc ggcgccggcg acggatcccg ctccgggacc accctggccg
     8701 acgtcgccca ggtgctggat ctgcacccgg cgttccggcc ccgggcgtac gtcgactggc
     8761 gcgtcgagct cgccgacgac cggctggagc tcaccctcgg cgactgcccg gcgcggcacg
     8821 agcgtgggat ggagacctgg atcagtcggc tggccgacgg ccacgacgac ggcctggccg
     8881 ccgcggtcga gggggtgacg ctatacgccc ggctggagcg gaccggcccg tcgcgctggt
     8941 cggtcaccct ggccgacgag cccgcaccgg accgctccga ggttgcactc acccgcttct
     9001 ccaccggcgc caccttcgtc ttcatccgat ataggggtgc tcagtgacac accttgtcgc
     9061 aaatccgacc ggagaatccg acgatcccgc gatgcgggcg ttcgtcggcc cgaccggact
     9121 gctggtcgag ggcgcaacga gcggtccgtt ggccggcacg accctggcgg tcaaggacgt
     9181 catcgacgtc gccggtcggg tgaccggggc cggcaacccg gacctggccg cgtcccgggc
     9241 cccggcgggg cgatcggccc cggtcgtcga ggcgctggtt gcggccgggg cgacggtggt
     9301 gggcaagacg gtgaccgacg agctcgccta ctccctcggc ggcgacaacg agcacttccc
     9361 gccgccggtc aacccggccg cgccggaccg gaccaccggc gggtcgtcgt cgggctcggc
     9421 gtccgcggtg gcagccggcg ttgtcgacct ggccctgggt accgacaccg gcgggtcgat
     9481 ccgggtgccc gccagctact gcgggatcct cgggtggcgg ccgacccacg gggcggtcag
     9541 cgccgagggg gtgaccccgt tggcctccag cttcgacacc atcgggctgt tcgctcggtc
     9601 cgctccgctg ctgcgcgccg cggccggggt ggccctcgcg acggtcgccg gacgtcccgt
     9661 gtcgccgtcg ccggtcgagc ggttcgcgat cgtcgccgag ctgatggatc tgatcgggcc
     9721 acccgggacc gatgtgaggg acgccctcga gggggctgtc ctcgatcggt tcggagcggc
     9781 ggtgcccacc atccgcctcg ggtgcgacat ggacgagtgc ctggccacct tccggaccct
     9841 gcagggggcc gaggcgtggg ccacccacgg tgggtggatc cgatcatccc gcccgaggat
     9901 gggggagaac acggcgtacc gcttcgacgc cgcgtcgaag gtcaccgccg aacaggtcgc
     9961 cgccgcccga ccggtgcggg aacgggtccg ccgggcggtc cacgacgcga ccgtcggggg
    10021 gacggtcctt ttggcccccg ccgctgcagg cccggccccg gtccgtgggg agtacggacc
    10081 cgaggccgaa cgcatccggg ccgacacgct gcgctcgacc gtg
//
LOCUS       DAOQOD010000111        10110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2407_length_10110_cov_10.744903, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000111 DAOQOD010000000
VERSION     DAOQOD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_10110_cov_10.744903"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLZ72_11025"
     CDS             complement(<1..1064)
                     /locus_tag="PLZ72_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HNA36872.1"
                     /translation="MRRLLLVATVAALVLVAACSGGEDSTSAPGRPAHDTSTGREPAP
                     ASPPLVTDPEGPVRVPVSGDTTWVHDPSIAREDGTYHLYSTHDGIQHRTSTDLQHWSR
                     AAPVFAEPPVWLTQEAPVGKGDIWAPDISRWGGRWHLYYSTSEFPAGSLPTRNSAIGH
                     ATAVTLDPSSPDGGWTDHGPVLRSRGQFLDADRSGWNAIDPAVVLDDDNRPWLSWGSN
                     YDGIFLQPLQPDGTLDPSVPPVNLARRELVTDNIETPTILKRGDRWYLFASFDLCCFG
                     MNSTYNVRVGRSASLAGPYVDRDGRPMLEGGGTKVLATYGNVLAPGHQTVLHEGDNWW
                     LIHHWYDPAHDAHPELGIRPL"
     gene            1193..1960
                     /locus_tag="PLZ72_11030"
     CDS             1193..1960
                     /locus_tag="PLZ72_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36873.1"
                     /translation="MSTFAEVVWASLERGADQDPPRFDAGTLGHLPPPAQRFLGSALP
                     DGVPLHDVVHLSMSGEIKLAGRWLPFTAEQILRAGVGFVWAPVVGGRLIRFVGADALG
                     PGGARIEFRLYGRIPIVRGTGPDIARSAHGRLAAETVAWLPTALTPQAGARWTAVDQH
                     RATVTLRGPGDPTDIQITVGGDGALRSMSLQRWKDSAKPPAFAPFGGTVDSGFVTGEG
                     IRVAGSGAAGWDWDTPRAGDGSFFRYEISSAELGHGA"
     gene            1953..2600
                     /locus_tag="PLZ72_11035"
     CDS             1953..2600
                     /locus_tag="PLZ72_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36874.1"
                     /translation="MPDPGEPVGADGEPVADAADAEGTAVDLYWIPLGAGTPVVQMSG
                     RLFERVTSWRHHREPCDLYHAALVLASPSGRYMIEQAPEPDSDGAARGVVAVGPVGLR
                     IAGRLRWFRYEVRCWRDGVIPDLDAAVDSPVRVSTRPDLRVRIIEHLPSVPTAVWGRD
                     EARAGEMWNSNSVLSWVLTRSGIDTTELNPPSGGRAPGWSAGRIVALRGPVTPGR"
     gene            complement(2624..3760)
                     /locus_tag="PLZ72_11040"
     CDS             complement(2624..3760)
                     /locus_tag="PLZ72_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36875.1"
                     /translation="MSLTTTTTPNLPLLERAARVGDEIAAHAERHDRDATFVVEAYDA
                     LRREGLLAAAVPVELGGAGASIAEVTAMQQELGRHCGSTALASAMHQHVTAFTAWRHR
                     RGLPGAEATLRRVAEDGIVLVSTGGADFTHPRGQAVRVDGGYRVSGTKAFASQGPVGT
                     AMSTMFPFEDPERGTRVLNMSVPLSEPGVRLVDNWDTLGMRGTGSGDIVVDDVFVPDE
                     RVLADRPHGTIDPPLQVILSIAMPIISGAYLGIARSAVDAATIAAAGSQDPITQRQIG
                     LMRHRVQVAQWALDGALEVIAEVPDPSEERMMAALTAKREVALAAVEVCDLAMEVAGG
                     SAFRKGSVIERAYRDVRAASFHPLTPEQTLRRSGQLAVGVPTDL"
     gene            3868..4605
                     /locus_tag="PLZ72_11045"
     CDS             3868..4605
                     /locus_tag="PLZ72_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA36876.1"
                     /translation="MTDYGRFCPIALASEVVADRWTPLILRELVLGNTRFNDIARGLP
                     GISRSLLVSRLKHLERKGVVESWPLPSGRGSSYHLTPAGRDLQPVLIAFGRWAVNWLY
                     DDLDPGDIDPITLTWWMHRRVDPTELPEQRVVVQFDHTAPEKMTLWMVLERGEASVCV
                     QHPGFDSDVVVTMETTALAEVFAGTVEWRRAVADGRIEVVGPSHLVRALPRWFLWSPF
                     WDVTRERSAATAPLVAASERADQQEVS"
     gene            complement(4660..5064)
                     /locus_tag="PLZ72_11050"
     CDS             complement(4660..5064)
                     /locus_tag="PLZ72_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNA36877.1"
                     /translation="MGASGHCLCGKVTYELSGEPIATAVCHCSHCQRQSGGAFSVNVI
                     ALPDQLTVTGELGTYVETGENDDGEYVHRRFCPSCGSPIVSEMLQAGIVAVKAGTLDD
                     TSDIRPAVEVWCTDRQPWVELAGIPMSMEREG"
     gene            5248..6981
                     /locus_tag="PLZ72_11055"
     CDS             5248..6981
                     /locus_tag="PLZ72_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA36878.1"
                     /translation="MIGPAILAVDADPDRLGEIERELTERYARSYSVAVAGSADDAHR
                     LLEQLAVDGRDVAIALCALDVGGVDGADILDGVRSRHRHARRGLLVRWESLGQRDVGE
                     IIFQAMAADRIDQFVFRPMVSPDEQFHSAVSSMLLDWADAQRTAPNTVRIVGESWTGR
                     AHELREALQSCAVPHDFCLVDSPNGRATLEVAGVDAELPLLQFPDGSILQNPTNAELA
                     LATGGPVCPEQLEFDVVVVGAGPAGLSAAVYGASEGLGTLVVDRGGIGGQATSSSLIR
                     NYLGFPRGLSGRRLAQNAYHQAWVFGADFTFMHTVTDIRREGRLLVLTLDDGTELRTV
                     GVLLAMGATYQRLGVPSIEAMVGAGVFYGSSTSEAVSMTGAHAFIVGGANSAGQAALH
                     LARYARQVTMVIRADSIEKGMSHYLIRQIDATPNIDVRTGTTVVDGGGVDRLEWLTLR
                     DGITGEQERHPAQGLFLMIGARPLTDWLPESIALDPQGFILSGDDVVGSGAWPLERPP
                     LTLETSMPGVFAAGDVRHGGVRRVASAVGEGSIAIQQLHQYFALEGRSPVGRQRADDP
                     NLPDQAGESGP"
     gene            7024..7461
                     /locus_tag="PLZ72_11060"
     CDS             7024..7461
                     /locus_tag="PLZ72_11060"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA36879.1"
                     /translation="MPSTLTVLEVIAEPTRRRILDAVRSGERSVGELVDEVGMHQPGV
                     SRHLKVLRDAGLVEVRRDAQRRLYRLRPEPLMEIDEWLEPYRREWANRLDSLERHLER
                     TADPIDPTIGSRTTQHSRPKDSRPKDSRTKDSRTKDSRTKDSR"
     gene            7458..8024
                     /locus_tag="PLZ72_11065"
     CDS             7458..8024
                     /locus_tag="PLZ72_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA36880.1"
                     /translation="MTDPGSHGGTGSHDGAGSYDGTLERTATGGTIRFERHLRFPVAA
                     VWDAITNPERLADWWLPFDADITVDLREGGEMVFTSRSGEPGPMSATVLRVEAPVLFE
                     HTHIVAGSTLLWELEAADDGCVLRLTQHVDDVAAAVDGCWVVGLHTSLARLAPALSGS
                     PVPWDWDAFAAARVHYAAIGAAAAEPGS"
     gene            8072..8719
                     /locus_tag="PLZ72_11070"
     CDS             8072..8719
                     /locus_tag="PLZ72_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNA36881.1"
                     /translation="MSQLLRVQNFMVSRDGFGTGEGQSLERPFGHADPAQLASWAGAT
                     ASWPNRTDLGGTRGLDDYFTRDFTHNIGAEIMGRNKFGPQRGPWEDHEWKGWWGDVPP
                     FHTPVFVLTHHPRPSFTLDDTTFHFIDASPAEAFARAAEAADGRDVRLGGGVATVREF
                     LDDDLVDAMHVAMAPVDLGRGERLWDRPEDLLDRFHMEKVPSPSSGVVHHIFWRR"
     gene            8787..9893
                     /locus_tag="PLZ72_11075"
     CDS             8787..9893
                     /locus_tag="PLZ72_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNA36882.1"
                     /translation="MLTVRDIRRLRLRNQWLGSEPDASAEQVVTHLGALQSQELWSGE
                     WSIGSRSTSVDAAAVHQATLDRGILRTWPMRGTIHFVPAVDAGWMLELGRTSAFKGVE
                     RRRQFLGLSEADAFAAIEVLRDALADGEPVTRAECTRILEEAGLMADRSHAYHLLWFA
                     AQHGATCIGPQLGKEQTFVALRSWVPHLRELTRDEALAELAFRYFDSHGPADRKELGR
                     WSALPAADVRRAISLAGDRLVPLDTELGEMLVSVSVADGLDGTPIEPPASKVLLLSGF
                     DEYMLGYGDRSAIMSPEQMDLVVPGSNGVFRPTVVDDGVVVGTWKRTVKRTRIEILVS
                     PFAAFAARQRSGVERAAEGYGRFLGLDSAVTFAD"
BASE COUNT         1471 a         3493 c         3609 g         1537 t
ORIGIN      
        1 agcgggcgga tgccgagctc gggatgggcg tcgtgcgcgg ggtcgtacca gtggtggatc
       61 agccaccaat tgtcaccctc gtgcaacacc gtctggtgtc ctggggcgag cacgttcccg
      121 tacgtggcca gcaccttcgt gccgccgcct tcgagcatcg gtcgaccgtc gcgatcgacg
      181 tagggccccg ccagggaggc ggagcgaccg acccgcacgt tgtaggtcga gttcatcccg
      241 aagcagcaca ggtcgaacga agcgaagagg taccaccggt caccccgctt gaggatggtc
      301 ggcgtctcga tgttgtcggt gaccaactca cggcgggcga ggttgaccgg gggcaccgac
      361 gggtcgaggg ttccgtcggg ctggaggggc tgcaggaaga tgccgtcgta gttggacccc
      421 catgacaacc acgggcggtt gtcgtcgtcg agcacgaccg cagggtcgat ggcgttccac
      481 cccgaccggt ccgcatccag gaattgcccc cgcgaccgga gcaccgggcc gtggtcggtc
      541 catccgccgt cgggtgacga cggatcgagg gtcaccgcgg tcgcgtggcc gatggcggag
      601 ttgcgggtcg gcaaggagcc ggccgggaac tcgctggttg agtagtagag gtgccaccgg
      661 ccaccccagc ggctgatgtc gggtgcccag atgtcgccct tgccgaccgg agcctcctgg
      721 gtcagccaca cgggcggctc ggcgaacacc ggcgccgccc tggaccagtg ctggaggtcc
      781 gtggatgtgc ggtgctggat gccgtcgtgg gtggagtaga ggtggtacgt gccgtcctca
      841 cgcgcgatcg acgggtcgtg cacccaggtc gtgtcgcccg acaccggtac tcgaacgggt
      901 ccctcggggt cggtgaccag cggcggggat gccggcgccg gttcgcgacc ggtgctggtg
      961 tcgtgggcgg gtcgacccgg agccgacgtc gagtcctctc ctcccgagca ggccgccacg
     1021 aggaccagcg cggcgacggt cgcgaccagc aggaggcgtc tcacgaatcg gcgaggctag
     1081 tcacgggctg accgagcctc cccgcaggac ggtcgcccgc ccctaacctc cgttctgttc
     1141 gttgtttcac aacgtcgacg ttgtgaaaca acgaacagaa cggaggtcga ccgtgagcac
     1201 cttcgctgag gtcgtgtggg cctcgctcga acgcggggcc gaccaggatc ccccgcggtt
     1261 cgacgcgggg acgctcggcc acctcccgcc accggcgcag cggttcctgg gctcggcgct
     1321 gccagacggc gtccccctgc acgacgtcgt gcacctgtcg atgagcggcg agatcaagct
     1381 ggcaggtcgg tggctgccct tcaccgccga gcagatcctc cgcgccgggg tcgggttcgt
     1441 gtgggctccg gtcgtcgggg gccgcctcat ccgcttcgtt ggtgccgacg cgctcggccc
     1501 cggaggtgcc cgcatcgagt tccggctgta cgggcgcatc ccgatcgtgc ggggaaccgg
     1561 tccggacatc gcccgcagcg cccacgggcg cctggccgcc gagaccgtcg cctggcttcc
     1621 caccgcgctc acccctcagg ccggggcgcg ctggacagcg gtcgaccagc accgcgccac
     1681 cgtcaccctc cgagggccgg gagatccgac cgacatccag atcaccgtcg gcggcgacgg
     1741 agcgctcaga tcgatgtcgc tgcagagatg gaaggactcc gccaagccgc cggccttcgc
     1801 tccgttcggc ggcaccgtcg actccgggtt cgtgaccgga gaggggatcc gggtggcggg
     1861 ctcgggtgct gccggttggg actgggacac gccgcgagcg ggcgacgggt cgttcttccg
     1921 ctacgagatc tcgagcgcgg agctcggaca cggtgcctga cccgggagaa cccgtcggtg
     1981 cggacggcga gccggtcgcc gacgcagccg acgcggaggg gaccgccgtc gatctgtact
     2041 ggatcccgct cggggcgggc acaccggtgg tgcagatgag cggccggctg ttcgagcggg
     2101 tcacctcgtg gcgtcaccac cgggaaccgt gcgacctgta ccacgccgct ctggtgctcg
     2161 cgtcgccgtc gggccgctac atgatcgaac aggctcccga accggacagc gacggcgctg
     2221 ctcggggtgt ggtcgccgtg ggtccggtgg gactgcgaat cgcgggtcgg ctgcggtggt
     2281 tccgctacga ggtccgatgc tggcgtgacg gcgtcatccc cgacctcgac gccgcggtcg
     2341 acagcccggt ccgggtctcg acccgcccgg atctgcgggt ccggatcatc gagcatctgc
     2401 cctcggtccc caccgcggtg tggggtcgag acgaggcccg tgccggggag atgtggaact
     2461 cgaactcggt cctgtcctgg gttctgaccc gcagcggcat cgacaccacc gagctgaacc
     2521 cgccatcggg tgggcgagcg ccgggctggt ccgcgggccg tatcgtcgcc ctccggggtc
     2581 cggtcacccc gggtcggtga gacccaaggc cccgaccacg cgatcagagg tcggtgggta
     2641 ccccgacggc gagctggccg gatcgacgca gcgtctgctc cggcgtgagc ggatggaacg
     2701 acgcggcccg gacgtcccgg taggcgcgct cgatcaccga gcccttgcgg aacgcggatc
     2761 cgccggccac ctccatggcc aggtcgcaca cctctaccgc cgcgagggcg acctcgcgct
     2821 tggcggtgag cgcggccatc atccgctcct cagacggatc gggaacctcc gcgatcacct
     2881 cgagtgcgcc gtcgagcgcc cactgggcca cctggacccg gtggcgcatc aggccgatct
     2941 gtcgctgggt gatcggatcc tggctgccgg cggcggcgat cgtcgcggcg tccaccgcgg
     3001 accgggcgat gcccaggtag gcgcccgaga tgatcggcat ggcgatgctg aggatcacct
     3061 gcagtggcgg atcgatcgtg ccgtgcggcc ggtcggcgag gacccgctcg tcggggacga
     3121 acacgtcgtc gacgacgatg tcgccgctcc cggtgcctcg catccccagc gtgtcccagt
     3181 tgtcgacgag gcggacgccg ggctcgctca gcggcaccga catgttgagc acgcgagtgc
     3241 cacgctcggg atcctcgaac gggaacatcg tggacatcgc tgtgccgacg ggcccctggc
     3301 tggcgaaggc cttggtgccc gagacccggt agcccccgtc gacccgcacc gcttgcccac
     3361 gggggtgggt gaagtcggcg ccgccggtgg agacgagcac gatgccgtcc tccgcgacac
     3421 ggcgcaaagt cgcttcggca ccaggcagac cgcggcggtg gcgccatgcc gtgaacgccg
     3481 tgacgtgctg gtgcatggcg ctggccaggg cggtcgaacc gcagtgccgg ccgagctcct
     3541 gttgcatcgc ggtgacctcg gcgatggacg cgccggcacc gccgagctcg acaggcacgg
     3601 ccgcggccag caggccctcg cgtcggagcg cgtcgtaggc ctcgacgacg aacgtggcgt
     3661 cgcggtcgtg acgttcggcg tgcgccgcga tctcgtcgcc gacccgggcc gcccgttcga
     3721 gcagggggag gttgggggtg gttgtggtgg tgagtgacat ggttcgagca tcggaccgcg
     3781 gcgccgccct gcccagtgca ggatcgtgac ctggccggta cagaatcggg acttcccgca
     3841 gcgctgcgtc ggcgcgacgc tcagggcatg accgactacg gccgcttctg tccgatcgcc
     3901 ctggccagcg aggtcgtggc cgatcgctgg acgccgctga tcctgcgaga gctcgtcctg
     3961 ggcaacaccc ggttcaacga catcgcccgc ggactgccgg gcatctcccg ttcgctgttg
     4021 gtgtcgcggt tgaagcacct ggagcgcaag ggtgtggtgg agagctggcc gctgccgtca
     4081 ggccgcggta gctcgtatca cctgaccccc gccggtcgtg acctgcagcc ggtgctcatc
     4141 gccttcggtc ggtgggcggt caactggctc tacgacgacc tcgaccccgg agacatcgac
     4201 ccgatcaccc tcacctggtg gatgcaccgg cgcgtggatc cgaccgagct gcccgagcaa
     4261 cgggtcgtgg tgcagttcga ccacaccgcg cccgagaaga tgacgctgtg gatggtgctg
     4321 gaacgggggg aggcgtccgt gtgcgtgcag catcccgggt tcgactcgga cgtggtcgtc
     4381 accatggaga ccaccgctct ggcggaggtg ttcgccggga ccgtcgaatg gaggcgggcg
     4441 gtggccgacg gtcggatcga agtggtgggg ccgtcgcatc tggtccgcgc cctgccccgc
     4501 tggttcctgt ggagcccgtt ctgggacgtc acccgggagc gaagcgctgc aacagctccg
     4561 ctggtcgccg cctcggagcg cgccgaccag caggaggtgt cctagtcccg gacgcggatc
     4621 gtgctcggag ggcgggtgcc ggctccgagg cgatcgtcct cagccctcgc gctccatcga
     4681 catcgggatc cccgccagct cgacccacgg ctggcggtcg gtgcaccaca cctcgacggc
     4741 gggtcggatg tcggaggtgt cgtcgagggt gcccgccttg accgcgacga tgccggcctg
     4801 gagcatctcc gacacgatcg gcgaaccgca ggacgggcag aagcgtcgat ggacgtactc
     4861 gccgtcgtcg ttctcgccgg tctcgacgta ggtgccgagc tcgcccgtga ccgtgagctg
     4921 gtccggcagg gcgatcacgt tcaccgagaa cgctccgccg ctctggcgtt ggcagtggct
     4981 gcagtggcac accgctgtcg cgatcggttc acccgacagc tcgtaggtca ccttgccgca
     5041 caggcaatgt ccggacgcgc ccatggcgtg gccctcctcg ggagtcgttc gaccggtacg
     5101 tgccgtggca cggaccgcgc tggagatcgc gccaccctac ggtcggcccg ccgtccgggg
     5161 tcgacagttc gtcgagatcc gagagctgca ccgacgacgt cgtgaaattc cggccgcggg
     5221 gacggggtac ggtcgtagat tctcgccatg atcggtcccg cgatcctcgc cgtcgacgcc
     5281 gaccccgacc gcctcggtga gatcgagcgc gagctgaccg agaggtacgc ccggagctac
     5341 tcggtcgcgg tcgccggctc agccgacgac gcgcatcgac tgctggagca gctcgccgtc
     5401 gacggacggg acgtggcgat cgccctctgc gcgctcgacg tcggtggtgt cgacggcgcc
     5461 gacatcctcg acggcgtgag gagccgacac cgccacgctc gccgcggcct gctggtgcga
     5521 tgggagtccc tcggccagcg tgacgtcggc gagatcatct tccaggcgat ggccgcggac
     5581 cggatcgacc agttcgtgtt ccgaccgatg gtgtcgccgg acgagcagtt ccacagcgcc
     5641 gtctcgtcga tgctgctcga ctgggccgac gcgcaacgca ccgcgcccaa caccgttcgc
     5701 atcgtcggcg agtcctggac ggggagggcg catgagctgc gcgaggcgct ccagtcgtgc
     5761 gccgtccccc acgacttctg cctggtcgac tcgccgaacg gtcgggcaac cctcgaggtc
     5821 gcaggcgtcg atgccgagct cccgctgctg cagttccccg atgggtcgat cctgcagaac
     5881 cccaccaacg ccgagctcgc cctggccacg ggcggtccgg tgtgcccgga gcagctcgag
     5941 ttcgacgtcg tcgtggtggg tgccggaccg gccgggttgt ccgctgcggt ctacggagca
     6001 tcggaaggcc tgggaaccct ggtggtcgat cggggaggga tcggtggaca ggccacctcg
     6061 agctccctga tccgcaacta cctcggtttc cctcgtggtc tcagcggccg tcggctcgcc
     6121 cagaacgcct accaccaggc gtgggtgttc ggcgccgact tcaccttcat gcacaccgtg
     6181 accgacatcc ggcgtgaggg ccggctgctg gtgctgaccc tcgacgacgg gacggagctg
     6241 cgcacggtcg gtgtgctgct ggcgatgggt gctacctacc agcggctcgg agttccgtcg
     6301 atcgaggcga tggtcggcgc cggggtcttc tacggcagct cgacctcgga ggcggtgtcg
     6361 atgaccggtg cgcacgcgtt catcgtcggc ggggcgaact cggcgggcca ggctgcgctc
     6421 cacctcgccc gatacgcccg gcaggtcacg atggtcatcc gggcggactc gatcgagaaa
     6481 gggatgtcgc actacctcat ccgccagatc gacgcgacgc ccaacatcga cgtgcggacc
     6541 gggacgacgg tcgtggacgg cggcggggtg gaccgactcg agtggttgac gctgcgcgac
     6601 gggatcaccg gtgagcagga acggcatccc gcgcagggcc tcttcctgat gatcggggcc
     6661 cgaccgctga ccgactggct gccggagtcg atcgccctcg acccccaggg gttcattctg
     6721 agcggcgacg acgtcgtggg ttcgggagcc tggccgctgg agcgccctcc gctcaccctc
     6781 gagacgagca tgccgggggt gttcgctgcg ggcgacgtgc gccacggcgg cgtccggcgg
     6841 gtcgcgtccg ccgtcggaga gggctcgatc gccatccagc agctgcacca gtacttcgcc
     6901 ctcgagggga gatcgcccgt cggccgacag cgcgcggacg atccgaactt gcctgatcag
     6961 gccggggagt cgggtccgta acatctgctt gcacatatat atggacagga gtatgttcag
     7021 gacgtgccat cgaccctgac cgtcctcgag gtgatcgcgg agccgacacg gcgccggatc
     7081 ctcgacgccg tccgatccgg cgagcgctcg gtgggggagt tggtcgacga ggtcggcatg
     7141 caccagccgg gggtgtcgcg gcatctgaag gtgctgcgtg acgccggcct ggtcgaggtc
     7201 cgccgtgacg cacaacgtcg gctctaccgc ctccggcccg agccgctgat ggagatcgac
     7261 gagtggctcg agccctaccg gcgcgagtgg gcgaaccgcc tcgattccct cgagcggcat
     7321 ctcgaacgca ccgctgaccc gatcgacccg acgattggct cccgaacgac gcagcactcc
     7381 cgaccgaagg actcccgacc gaaggactcc cgaacgaagg actcccgaac gaaggactcc
     7441 cgaacgaagg actcccgatg accgaccccg gatcccacgg tggcacaggc tcccacgacg
     7501 gcgcaggctc ctacgacggc accctcgaac gaaccgccac tggcggcacg atccgcttcg
     7561 aacgacacct gcggttcccg gtggctgcgg tgtgggacgc catcaccaac ccggagcggc
     7621 tcgcggactg gtggctgccc ttcgatgccg acatcaccgt cgacctacgc gaaggcgggg
     7681 agatggtatt caccagccgc agcggcgaac ccggcccgat gagcgccacc gtgctgcggg
     7741 tggaggcacc cgtcctgttc gagcacaccc acatcgtcgc gggatccacc ctgttgtggg
     7801 agctcgaggc cgccgacgac ggatgcgtgc tccgtctcac ccagcacgtc gacgacgtgg
     7861 ccgccgcggt cgacgggtgc tgggtcgtcg gactgcacac atccctggcg cgactcgcac
     7921 cggccctgtc cggctcaccg gtgccctggg actgggacgc cttcgccgcc gcgcgcgtgc
     7981 actacgccgc catcggtgct gccgccgccg agcccgggtc ctgagacccg acgaacgtga
     8041 ccgaacacac cagagcgatg aggaaccgcc gatgagccag ctgctgcgag tccagaactt
     8101 catggtgtcc cgagacggct tcggcaccgg tgagggtcag tccctcgagc gcccattcgg
     8161 acacgcggat cccgcccagc tcgcctcctg ggcaggggcg acggccagct ggcccaaccg
     8221 caccgacctg ggcgggacgc gtggcctcga cgactacttc acccgcgact tcacccacaa
     8281 catcggcgcc gagatcatgg ggcgcaacaa gttcggaccg caacgggggc cttgggagga
     8341 ccacgagtgg aaggggtggt ggggcgacgt cccgccgttc cacaccccgg tgttcgtcct
     8401 gacccaccat ccccgtccgt cgttcaccct cgacgacacc acgttccact tcatcgacgc
     8461 atcgccggcc gaggcgttcg cccgagccgc ggaggccgcg gacggcaggg acgtgcgcct
     8521 gggcggggga gtcgcgaccg tgagggagtt cctcgacgac gatctcgtcg acgccatgca
     8581 cgtggcgatg gcgccggtcg acctcgggcg cggcgaacgc ctgtgggacc ggcccgagga
     8641 cctgctcgac cgcttccaca tggagaaggt gcccagtccc agctccgggg tcgtgcacca
     8701 catcttctgg cggcgttgag caccgcccac gacctcaccc tggtcctcgc gtcgagcgag
     8761 ggacgtgatg cggcatcatc gacgccgtgt tgaccgtccg cgacatccgg cgactccgac
     8821 tgcgcaacca gtggctcggg tcagaacccg acgcgtcggc tgaacaggtc gtcacccacc
     8881 tcggcgcgct gcagtcccag gagttgtgga gcggagagtg gtcgatcgga tcgaggtcga
     8941 catcggtgga tgccgccgcg gtgcaccagg cgaccctcga tcgcgggatc ctgcggacgt
     9001 ggccgatgcg aggcacgatc cacttcgtcc ccgccgtcga tgccggctgg atgctcgagc
     9061 tgggtcgtac ctcggcgttc aagggcgtgg agcggcgccg ccagttcctc ggtctcagcg
     9121 aggccgatgc gttcgccgcg atcgaggtcc tgcgagacgc gctggccgac ggtgagcccg
     9181 tgacccgggc cgagtgcacc cggatcctgg aggaggcggg tctgatggcg gaccggagtc
     9241 acgcatacca cctcctgtgg ttcgcggcgc agcacggggc gacgtgcatc ggcccccagc
     9301 tcggcaagga acagacgttc gtggctctcc gctcgtgggt tccccacctc cgggaactga
     9361 cccgcgacga agccctggcc gagctcgcgt tccgctactt cgacagccac gggccggcgg
     9421 accgcaagga gctcggccga tggagcgccc tgcccgccgc ggatgtgcgc cgcgcgatct
     9481 ccctggccgg ggaccggctc gtgccgctcg acacggagct cggcgagatg ctcgtcagcg
     9541 tctcggtggc cgacgggctc gacggcaccc cgatcgagcc acccgcctcc aaggtgctgc
     9601 tgctgtcggg gttcgacgag tacatgctcg gctacggcga ccgttcggcg atcatgtcgc
     9661 ccgaacagat ggatctcgtc gttccgggct ccaacggcgt cttccgaccc acggtcgtcg
     9721 acgacggcgt ggtggtcggc acatggaagc ggacggtgaa gcgcacgcgg atcgagatcc
     9781 tggtctcacc gttcgcggcg ttcgcggccc ggcaacgctc cggcgtcgaa cgggccgctg
     9841 agggctacgg ccggttcctc ggcctcgact ccgccgtcac cttcgccgac taggtgttcc
     9901 ggctcgacag cggggtcgat gcgcctagtg tccgcgccgt ggacgcgacg caggacgacg
     9961 gccaggtgga gtcggagccg gagcaggagc cgggctcgtc gggtggcgac ccgcgtcggc
    10021 cggcggtgca caccttcgac gacgagctcg cccgactcga cgcggtggcg acgatcgacg
    10081 cgctcgactc gggccagctg tcggtccggg
//
LOCUS       DAOQOD010000112        10082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2424_length_10082_cov_8.962601, whole genome shotgun sequence.
ACCESSION   DAOQOD010000112 DAOQOD010000000
VERSION     DAOQOD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_10082_cov_8.962601"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLZ72_11080"
     CDS             <1..879
                     /locus_tag="PLZ72_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36883.1"
                     /translation="AERPRRGGPRDAADDPASALDGNPLRQLGVIGPGAVLDSAFALL
                     RFRFGRLLALTAVVVVPAQALDLVMALRFGTPSTAEDLPQLQLLDTGSSLPFWSTLTV
                     LALRAIGLFLLGMAVGVLVAGWLDGRDETFGTVVGAVARRAWVVPVVVLVAGTARFAA
                     ACAGGVGFFLVDALLFIAAPIAAVERSGPWATLGRSLRLTRSAYGNALVICVGGFVIA
                     SVVRVALALGPAALISMLGLPEGWMLVVEQAGSLTRLITLPLIACIAARAYIDLRCRA
                     EGFDLLRRQEARALVA"
     gene            863..1630
                     /locus_tag="PLZ72_11085"
     CDS             863..1630
                     /locus_tag="PLZ72_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNA36884.1"
                     /translation="MRWSRERVPASIRILVAALATIVVAAAVSTAAGAQGSDPDVPVS
                     SEQARAAVRDVMARPEFDYSPSVLERISEWIGEQLSKLFAPAETAAGGSFGGGIGTLF
                     GWLIMIAAVAALVVVAVWVIANRSRRERGEDEDPLSPTEVEHRRRADEWRSDAERLES
                     QGRWKEALRARYRHLVRVLVDRRQLPDVPGRTTGELRGDLAATTPDAVDEFDTCCLLF
                     ELAWYADVPTDAEGNARFRTAAGRVLAAPVGSDREGA"
     gene            1627..2892
                     /locus_tag="PLZ72_11090"
     CDS             1627..2892
                     /locus_tag="PLZ72_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNA36885.1"
                     /translation="MTASAAAGRSGGAITWIGGAVLAVVVVALVVVTSRQEVVVAFDP
                     ASSAPDGYRALALLLRDRGAEVDSTAPVLLGTDPVPAGSVMVVPDPDLLTAVEYRAAL
                     ESAEAGATVVLGAPRRSGSDDPGGSDPDEPDPGVGGGVPAVPARVLADTPAEPTRQGA
                     CDIGRLDGLGPIDTAFGTPVTAGDAGAEARGCYGDVRGTHVLEEDHGAGTLITLSSPY
                     LWANARLQPDKEDGGEPLDNAAVALRLLGPTGAGATEGTSVTFVDAVPTAGVSPDGTR
                     NPIELLPTGVKLALVQLVAAFLLYAWWRSRRLGPVVVERMPVEIAGSELVVAVGDLMR
                     RKGSAQRAADVLRSDARRDLARGLGVPPDAPPETVVRVVSTRSGRDVDAVAAVLGDGP
                     VPSAEALVRLALSLSDIRQEVLENHVPPR"
     gene            2966..3904
                     /locus_tag="PLZ72_11095"
     CDS             2966..3904
                     /locus_tag="PLZ72_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA36886.1"
                     /translation="MPIVALRDEIGKVVVGQEGTLSGLIAALLVRGHVLLEGVPGVAK
                     TLLVKTLARALDLEFGRVQFTPDLMPSDVTGQLVLDRGSAEGMRFRQGPIFTNLFLAD
                     EINRTPPKTQSALLEAMEERQVTAEGVARALPDPFVVIATENPVEHEGTYPLPEAQLD
                     RFMFKLLVGYPTFEQEAEILARHHRGLDPHDVVAAGVRPVATATDLLRAREQVELVDV
                     QPTVQQYIVALCRATRDSPSVELGVSPRGAAALLHASKAWAWLSGRSFVTPDEVKAVA
                     KPTLRHRLIVRPELELDGVTADGLLDSILATVPAPR"
     gene            3927..5258
                     /locus_tag="PLZ72_11100"
     CDS             3927..5258
                     /locus_tag="PLZ72_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA36887.1"
                     /translation="MNLLPTRRLALLAVAVAAALLAFPLTGRAEDLPVWAAVAAVTGA
                     LVVLALLDAGIGTRPGQLVLERRHPPVLEVGREGQVTWVLRSEARRRLKVTVADELAP
                     SLGAGARRFDVSIPAGDAVRVATRIRPTRRGRFELDRLTIRIEGRLGLGARQRSVPLA
                     TVLRVHPAFPSREEAEIRIRRARILEVGLRSARGLGGGTEFEQLREYGPDDEFRRIDW
                     TATARAGKPIVRTYRAERNQSVVVVLDNGRIMAGRVADVPRVEHAMDAALMLTAVSTR
                     LGDRCGLVAFDRRVRAVVPPARHSDQLSRVSEALYRLEPELAESDYTGAFAEVVARYR
                     RRAMLVVLTDLNEQAVADSVLPALPLITRTHLVVVAAVQDPQVLRWAHRRPTDAEETF
                     RQVAALQALEDRRRTSARLRALGATVVDAAPGRLGVEVTDAYLLAKSTGRL"
     gene            complement(5255..>6378)
                     /locus_tag="PLZ72_11105"
     CDS             complement(5255..>6378)
                     /locus_tag="PLZ72_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36888.1"
                     /translation="TVENHHSMTFWARLENRGPWLESDVVVRSDTLEVHPLVGTVPVH
                     TVEIDGLTGVEDGPAVGPDERVVTLHSDDGTRLQLRGSALGVSSLVAAMGPWPDDTTD
                     DDATDGDATDGDATGSPPAEAGDPVDPLAPADRAQGPRWRFPAVVAGAAAFIVALGFW
                     NHSANQQELRELAARKHPTTSAPTTSTTTQRPPPTAAPTTALLPPPEVLGTTTIRPPA
                     STTVPTRPTTTAPRVPTTTAPIPTAPGTRLLIGSVSLRQVQHWGDTVPECRGVGQLSD
                     IGTGTAVVVRNAEGAIIAEGTLGGCRFVAPGSELSASTPAPQTDGSIGGIPRFDLQIA
                     DIADSSRYTVQVGRYDPVGFSRAEIGSGVWRIRMVISAG"
     assembly_gap    6379..6478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6479..7185)
                     /locus_tag="PLZ72_11110"
     CDS             complement(<6479..7185)
                     /locus_tag="PLZ72_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNA36889.1"
                     /translation="MDIDRYTQRHDPTWRRLQQLADRARSTRRRVDDDEIAELVSLYQ
                     RVSAQLSHARTTYADPALNARLSQLLGEARVVIYRRRSNPARAVATFATRTFPAAVWF
                     SRRFIAVAALAFLVPAFAFGIWLAHSPQVLDASIPPELQQVIAQKEFADYYRSDAAQN
                     FAGQVTVNNAFVAFLTFALGVVPVLGPVSVLFNNGLNVGVMGAVMHHAGEGPQFWGLI
                     LPHGLLEIASILVAGGAG"
     gene            7254..>8027
                     /locus_tag="PLZ72_11115"
     CDS             7254..>8027
                     /locus_tag="PLZ72_11115"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNA36890.1"
                     /translation="MHAGTVTPEAVLLELPTAGVATRAFARLVDLAVQLVAASVLGTV
                     LGFALPAGVSPVLMALVLMVAVLLVLPIAMEVASNGRSVGKAMFGLRAVGVDGSPVTT
                     RQSMVRGVVGLVDFYLSLGFLATVTAMFSTTSQRSGDMAAGTVVIRSRTDPAAAVPIA
                     FHPPVGYEHYVSTLDVGALDEEDFSLVRSFLLRVDQLQGDARRSIALGLAEGVRVRIA
                     HTPPAVIDPELFLVCVASAFQLRQGGLLADAALGLAPLAP"
     assembly_gap    8028..8127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8128..9397
                     /locus_tag="PLZ72_11120"
     CDS             <8128..9397
                     /locus_tag="PLZ72_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA36891.1"
                     /translation="DENPVPVLDHTGGDMDFDDSPEEIALRAEVRAWLDEHAVRRDPE
                     ALAGMRTFRTRTEAEDEALVAEARAWQRTKADSGWAAPSWPAELGGRDLSPLLAGVFA
                     AEEAHYDVVGRMFTVGTDMVGPTLIEWGTDEQRRHHLPRILRADDIWCQLFSEPGAGS
                     DLAGLSTRALRDGDEWVVTGQKVWTTGAHYSDLGILLARTDPDVPKHRGITAIAVDMR
                     APGVDVRPLRQIDGTIHFNEVFLDEVRVPVADTIGPVGSGWGVALTMLTNERASIGGG
                     SMYGVDQLVQLARESGRLGDPLIRQRLADMYIRDEILRFLGYRVRTAAASGRPPGPES
                     SVMKLAVSARYEVGGDLMLDIEGAAGALMGDDAPYGGRFQDLFMGQWAPRIGGGTDQV
                     QRNIIGERVLGLPGDIRVDKDVAFRDLPRA"
     gene            9431..>10082
                     /locus_tag="PLZ72_11125"
     CDS             9431..>10082
                     /locus_tag="PLZ72_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA36892.1"
                     /translation="MIPADDTTRPRPGTVGPDGPTVDFDRAATTPLRPEARAAMAPFL
                     AGRYGNPSSAHALGRDAMRAVDEAREQLADLLGCAPSELVFTSGGTEADVHAVTGGIP
                     TRADRVVCSAVEHPAVLATVTALGGSTVAVHRDGRIDLDALAAELSSPGKVGLVSVMA
                     ANNEIGTLNDLAEVAAVVAEHAPGAALHTDAVQAAAWLDLRRVATPAALVSVSAHKF"
BASE COUNT         1198 a         3535 c         3710 g         1439 t
ORIGIN      
        1 gccgagcgcc cgcggcgcgg cggacctcgc gacgcggccg atgacccggc ctcggcgctc
       61 gacggcaacc cgttgcgcca gctgggcgtg atcggtccgg gcgccgtgct cgactcggcg
      121 ttcgccctgc tgcggttccg cttcggtcga ctgctggccc tgaccgcggt cgtggtcgtc
      181 cccgcccagg cgctcgacct ggtgatggcg ctgcggttcg ggaccccgtc caccgccgag
      241 gacctgccgc agctccaact cctcgacacc ggatcctcgc tgccgttctg gtcgacgctc
      301 accgtgctgg ccctccgggc gatcgggctg ttcctgctcg ggatggcggt cggtgtcctc
      361 gtcgccgggt ggctcgatgg acgtgacgag accttcggga ccgtcgtcgg agccgttgcg
      421 cgtcgggcct gggtggtgcc ggtcgtggtg ctggtcgccg ggaccgcccg gttcgcggcc
      481 gcgtgcgccg gcggggtcgg cttcttcctg gtcgacgccc tgctgttcat cgccgccccg
      541 atcgcggcgg tggagcggag cggaccgtgg gcgacgctcg gccgcagcct ccgtctgacc
      601 cgcagcgcgt acggcaacgc actggtcatc tgcgtgggcg ggttcgtgat cgcgtcggtg
      661 gtccgggtgg cgctcgccct gggtcccgcg gcgctgatct cgatgctcgg cctgcccgag
      721 ggctggatgc tggtcgtcga gcaggccggg tcgctcacgc gtctgatcac gttgccgttg
      781 atcgcctgca tcgcggcacg ggcgtacatc gatctgcgct gtcgggccga gggcttcgac
      841 ctgctgcgtc gacaggaggc ccgtgcgctg gtcgcgtgag cgggtcccgg cgtcgatcag
      901 gatcctcgtc gccgctctcg ccacgatcgt cgtggcggcc gcggtgtcga cggccgccgg
      961 tgcccagggc tcggaccccg acgtcccggt ctcttccgag caggctcgcg ccgcggtgcg
     1021 cgacgtcatg gcgcggcccg agttcgacta ctcgccgtcc gtgctcgaac ggatctccga
     1081 gtggatcggc gaacagctct ccaagctgtt cgcgcccgcc gagaccgcgg ccggggggtc
     1141 cttcggcggc ggcatcggca cgctcttcgg ttggctgatc atgatcgccg cggtcgcggc
     1201 gctggtcgtg gtggcggtct gggtcatcgc caaccgcagc cgtcgcgagc gcggcgagga
     1261 cgaggacccc ctcagcccga ccgaggtcga acaccgtcgc cgggccgacg agtggcgctc
     1321 cgatgccgag cgcctcgagt cccagggtcg ctggaaggag gcgctgcggg cccgctaccg
     1381 gcacctggtc cgggtcctgg tcgaccggcg ccagctcccc gacgtacccg gccgcacgac
     1441 cggcgagctg cgcggcgacc tggccgccac caccccggac gcggtcgacg agttcgacac
     1501 ctgctgcctc ctgttcgagt tggcctggta cgcggacgtc ccgaccgacg cggagggcaa
     1561 cgcccgcttc cgaacggccg ccgggcgggt cctcgccgca ccggtcggct ctgacaggga
     1621 gggagcgtga cggcgtcggc ggccgcgggg cgcagcggcg gagcgatcac ctggatcggg
     1681 ggtgcggtcc tggccgtggt cgtggtcgct ctcgttgtcg tcacctcccg ccaggaggtc
     1741 gtcgtggcgt tcgatcccgc gtcgtcggcg ccggacggct accgcgccct ggctctcctg
     1801 ctgcgcgatc ggggtgcgga ggtcgactcg accgcgccgg ttctcctcgg cacggacccg
     1861 gtgccggccg ggtcggtcat ggtggtgccg gaccccgacc tgctcaccgc ggtcgagtac
     1921 cgggccgccc tcgagtcggc cgaggcgggc gcgaccgtgg tgctcggtgc cccccgacga
     1981 tccgggtccg acgaccccgg tgggtccgac cccgatgaac ccgacccggg cgtcggtggc
     2041 ggggtgcccg cggtcccggc ccgggtgctc gccgacaccc cggccgagcc gacccgccag
     2101 ggggcctgcg acatcggccg cctcgacggg ctcgggccga tcgacaccgc gttcgggaca
     2161 cccgtcaccg ccggggacgc cggcgcggag gcacggggct gttacggcga cgtccgcggc
     2221 acccacgttc tggaggagga ccacggggcc gggacgctca tcaccctgag ctcgccgtac
     2281 ctgtgggcga acgcccggct gcagcccgac aaggaggacg gcggcgaacc cctcgacaac
     2341 gcggccgtcg cgctgcggct gctcgggccg accggggccg gagcgaccga gggcacgtcg
     2401 gtcaccttcg tcgacgcggt gccgaccgcg ggtgtctctc ccgacggcac ccgcaacccg
     2461 atcgagctcc tgcccaccgg ggtgaagctg gcgctggtgc agctggtggc cgcgttcctc
     2521 ctctacgcgt ggtggcgctc ccggcggctc ggaccggtgg tggtcgagag gatgccggtc
     2581 gagatcgccg ggtccgagct ggtcgtcgcc gtcggcgacc tgatgcgtcg caagggcagc
     2641 gcgcagcgcg ccgcggacgt gctgcgctcc gatgcccgac gggacctggc gcgtgggctg
     2701 ggcgtcccgc ccgatgcgcc gcccgagacc gtcgtccggg tcgtgtcgac ccggtccggc
     2761 cgagacgtcg acgccgtcgc cgcggtactc ggtgacggcc ccgtcccttc cgccgaggcg
     2821 ctggtgcgtc tggcgttgtc gttgtccgac atccgtcagg aggtcctcga gaaccatgtc
     2881 ccacccaggt gaccacaccg cgggccatga cccccagggg tacccgccac ccgcatcgcc
     2941 cccgccgccg taccccgatc cgcgggtgcc gatcgtggcg ctgcgcgacg agatcggcaa
     3001 ggtggtcgtc ggccaggagg ggacgctgtc gggtctgatc gcggcgcttc tggtccgtgg
     3061 acacgtgctg ctcgagggcg tgcccggggt ggccaagacc ctcctggtca agaccctggc
     3121 cagagccctc gacctcgagt tcgggagggt ccagttcacc ccggacctca tgccgtccga
     3181 cgtcaccggc cagctcgtcc tcgatcgggg gtccgcggag gggatgcggt tccggcaggg
     3241 tccgatcttc accaacctgt tcctggccga cgagatcaac cggacgcccc ccaagaccca
     3301 gagcgccctg ctcgaagcca tggaggagcg gcaggtgacc gccgagggcg tcgcccgagc
     3361 gctgccggac ccgttcgtgg tgatcgccac cgagaacccg gtcgagcacg aaggcaccta
     3421 tccgctgccc gaggcccagc tcgaccggtt catgttcaag ctgctggtcg gctacccgac
     3481 cttcgagcag gaggccgaga tcctggcccg ccaccatcgg ggcctcgacc cccacgacgt
     3541 ggtcgcggcc ggggtccgcc cggtcgccac cgccaccgat ctgctccggg ccagggagca
     3601 ggtcgagctg gtcgacgtgc agcccacggt gcagcagtac atcgtcgccc tctgccgggc
     3661 gacccgcgac tcgccgtcgg tcgagctcgg cgtgtcgccc cgcggcgcgg ccgcgctgct
     3721 gcacgcatcc aaggcgtggg cgtggctgtc gggtcgcagc ttcgtcaccc ccgacgaggt
     3781 caaggccgtc gccaagccga ccctgcgcca ccggttgatc gtgcggcccg agctcgagct
     3841 ggacggcgtc accgccgacg gactgctcga cagcatcctg gccaccgtcc ccgcccctcg
     3901 ctgagcgaac gcgccgccgc tcccgcatga acctgctgcc cacccgtcgg ctggccctcc
     3961 tcgccgtggc cgtcgccgcg gccctgctgg cgttcccgct gaccggacgc gccgaggacc
     4021 tcccggtgtg ggccgcggtc gcggcggtca ccggggcgct cgtcgtgctg gcgctcctcg
     4081 acgccgggat cgggacccgc ccgggccaac tcgtgctcga acgacgccac cccccggtgc
     4141 tcgaggtcgg ccgggaggga caggtgacgt gggtgctgcg ctcagaggcc cggagacggc
     4201 tgaaggtgac cgtcgccgac gagctggccc cgtcgctcgg tgccggggcc cgacgcttcg
     4261 acgtgtcgat ccccgccggc gacgccgtcc gcgtcgccac ccgcatccgc ccgacgcgtc
     4321 gcggtcgctt cgagctcgac cggctcacga tccgcatcga gggccggctc ggcctggggg
     4381 cccggcagcg gtcggtgccc ctggcgaccg tcctgcgggt ccatccggcc ttcccgtcgc
     4441 gggaggaggc cgagatccgg atccggcggg cccgcatcct ggaggtggga ctgcgatcag
     4501 ctcgcgggct gggcgggggg accgagttcg agcagctccg cgagtacggc cccgatgacg
     4561 agttccgtcg catcgactgg accgcgacgg cccgggcggg caagccgatc gtgcgcacct
     4621 accgggcgga gcgcaaccag agcgtcgtgg tggtgctcga caacggtcgg atcatggcgg
     4681 gccgggtggc ggacgtgccc cgggtcgaac acgccatgga cgctgcgctg atgctgaccg
     4741 cggtgtccac ccggctgggc gaccgctgcg gtctggtcgc cttcgaccgt cgggtgcgcg
     4801 cggtggtccc acccgcacgg cactccgatc agctgagccg ggtgtccgag gcgttgtacc
     4861 ggctcgagcc ggagctggcc gagtcggatt acaccggcgc cttcgccgag gtcgtcgccc
     4921 ggtaccggcg tcgggcgatg ctggtggtgc tcaccgacct gaacgagcag gccgtggccg
     4981 actcggtgct cccggcgctg ccgttgatca ctcgcacaca cctggtggtg gtcgccgcgg
     5041 tccaggaccc gcaggtgctg cgctgggcgc accgtcggcc gaccgacgcc gaagagacgt
     5101 tccgccaggt cgccgcgttg caggcgctcg aggaccggcg acggacctcg gcccggttgc
     5161 gggcgctggg agcgaccgtg gtcgacgcgg cgccgggccg cctcggtgtc gaggtgaccg
     5221 acgcgtacct gctggccaag tcgaccggtc ggctctagcc ggccgagatg accatgcgga
     5281 tgcgccacac gccggagccg atctcggcgc gggagaagcc gaccgggtcg tagcgaccca
     5341 cttggacggt gtagcgcgac gagtcggcga tgtcggcgat ctgcaggtcg aatcggggga
     5401 taccgccgat cgacccgtcg gtctgcggag ccggggtgga cgcgctgagc tccgaaccgg
     5461 gcgccacgaa ccgacacccc ccgagagtcc cttccgcgat gatcgctccc tcggcgttgc
     5521 ggaccacgac cgctgtcccg gtcccgatgt cggagagctg accgacgccg cggcactccg
     5581 ggacggtgtc accccagtgc tggacctgtc gcagcgacac cgaaccgatc agcagccggg
     5641 ttccgggcgc ggtcgggatc ggtgcggtgg tcgtcggcac ccgcggggcg gtggtcgtcg
     5701 gccgggtggg caccgtcgtg gaggccgggg gtcggatcgt cgtggtgccc agcacctcgg
     5761 gcggcgggag cagagccgtg gtcggggctg cggtgggcgg gggtcgttgc gtggtcgtcg
     5821 acgtggtggg agcggaggtc gtcgggtgct tgcgggccgc gagctcgcgc agctcctgct
     5881 ggttggcgct gtggttccag aaccccagcg ccacgatgaa cgccgcggcg ccggccacga
     5941 ccgccgggaa gcgccagcgg ggcccctgag ctcgatccgc cggtgccagc ggatcgacgg
     6001 ggtcgccggc ttcggccggc ggggaacccg tcgcgtcgcc atcggtcgcg tcgccatcgg
     6061 tcgcgtcgtc atcggtcgtg tcgtcgggcc aggggcccat cgccgcgacc agcgagctga
     6121 cacccagggc gctgccacgg agctggagtc gggtcccgtc gtcggagtgg agggtgacca
     6181 cccgttcgtc ggggccgacc gccgggccgt cctcgacccc ggtcaggccg tcgatctcga
     6241 cggtgtgcac cgggaccgtt ccgaccagcg ggtggacctc caacgtgtcg gatcgcacga
     6301 cgacgtcgga ctcgagccac gggccccggt tctcgaggcg cgcccagaag gtcatgctgt
     6361 ggtggttctc aacggttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     6481 ggcgcctccg gcgacgagga tcgacgcgat ctcgagcagg ccgtggggca ggatcagccc
     6541 ccagaactgg gggccctccc cggcgtggtg catgaccgcg cccatcaccc cgacgttgag
     6601 cccgttgttg aacaggaccg acaccggacc gagtacgggc acgacgccca gagcgaaggt
     6661 gaggaacgcg acgaaggcgt tgttgacggt gacctgcccg gcgaagttct gagcggcgtc
     6721 ggagcggtag tagtcggcga actccttctg ggcgatcacc tgctgcagct cgggcgggat
     6781 gctggcgtcg agcacctgcg gcgagtgggc gagccagatc ccgaaggcga acgcggggac
     6841 caggaacgcg agcgcggcga ccgcgatgaa ccgacggctg aaccacaccg ctgccgggaa
     6901 ggtgcgggtc gcgaaggtgg cgacggcccg cgccgggttc gatcgcctcc ggtagatcac
     6961 cacccgggcc tcgccgagca gctggctcag gcgggcgttc agcgcgggat ccgcgtaggt
     7021 ggtgcgggcg tggctgagtt gggccgacac ccgctggtac agcgagacca gctcggcgat
     7081 ctcgtcgtcg tcgacccgcc ggcgcgtcga tcgggcccgg tcggccagct gctggagccg
     7141 acgccaggtc gggtcgtgtc gctgcgtgta gcggtcgatg tccatgtgct ggtggtcggc
     7201 tgtgctggtt cggtgcctgt gctgcccacg ctaggcccgt agcctcggtg tcgatgcacg
     7261 ccggaaccgt cacccccgaa gcggtcctgc tggagctgcc gaccgcgggt gtggccacga
     7321 gggccttcgc ccgcctggtc gacctggcgg tccagctggt ggcggcgtcg gtcctgggca
     7381 cggtcctcgg gttcgctctc ccggccgggg tcagcccggt gctcatggca cttgtgctga
     7441 tggtggcggt gctgttggtc ctgcccatcg ccatggaggt ggccagcaac ggtcgcagcg
     7501 tgggcaaggc catgttcggc ctgcgcgccg tcggggtgga cggatcgccg gtgacgacgc
     7561 gccagtcgat ggtccgcggc gtggtcggcc tggtggactt ctacctgagc ctcggcttcc
     7621 tggcgacggt caccgccatg ttctccacga cgtcgcagcg ctccggggac atggccgcgg
     7681 gcacggtagt catccgcagc cgcaccgacc cggcggccgc ggtgccgatc gcgttccacc
     7741 cgcctgtcgg ctacgagcac tacgtgtcga ccctcgacgt cggtgcgctc gacgaagagg
     7801 acttctcgct ggtccgttcg ttccttctcc gcgtcgacca gctgcagggc gacgcccgac
     7861 ggtcgatcgc gctcgggttg gccgaggggg tacgggtccg gatcgcccac acgccgcccg
     7921 cggtgatcga tcccgagctg ttcctggtgt gcgtcgcgtc ggcgttccag ctccgccagg
     7981 gcggcctcct ggccgatgcc gcgctcggcc tcgcgccact ggcccccnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnaga tgagaacccg gttccagttc tggaccacac
     8161 tggcggggat atggacttcg acgacagccc cgaggagatc gctctgcggg ccgaggtgcg
     8221 ggcctggctc gacgagcacg ccgtgcgacg ggatcccgag gcgttggccg gcatgcggac
     8281 cttccggacc cgcaccgagg ccgaggacga ggcgctcgtc gccgaggcgc gggcctggca
     8341 gcgcaccaag gccgacagcg gctgggcggc gccgtcgtgg ccggccgagc tcggcggccg
     8401 ggacctgtcg ccgcttctcg ccggggtgtt cgccgccgag gaggcccact acgacgtcgt
     8461 cggacgcatg ttcacggtgg gcaccgacat ggtcggcccg acgctcatcg agtggggcac
     8521 cgacgagcag cgccgccacc acctgccccg gatcctgcgg gccgacgaca tctggtgcca
     8581 gctgttcagc gaaccgggtg cgggatcgga cctggccggc ctgtcgaccc gggcgctgcg
     8641 cgacggcgac gagtgggtgg tcaccggcca gaaggtgtgg acgaccgggg cgcactactc
     8701 cgacctgggc atcctgctgg cgcggaccga ccccgacgtg cccaagcacc gggggatcac
     8761 cgcgatcgcc gtcgacatgc gcgctccggg cgtcgatgtc cgtccgctgc gtcagatcga
     8821 cggcaccatc cacttcaacg aggtgtttct cgacgaggtt cgcgtccctg tcgccgacac
     8881 catcggcccg gtcggctcgg gctggggggt ggcgctgacg atgctcacca acgagcgggc
     8941 ctcgatcggc ggcggctcga tgtacggcgt cgatcagctg gtgcagctgg cccgggagtc
     9001 cggccggctc ggggacccgc tgatccgcca gcggctggcc gacatgtaca tccgcgacga
     9061 gatcctgcgg ttcctcggct accgggtgcg gaccgcggcg gccagcgggc gtccccccgg
     9121 accggagagc tcggtgatga agctggcggt gtcggcccgc tacgaggtcg gcggcgacct
     9181 catgctcgac atcgagggcg cggcgggggc gctgatgggc gacgacgccc cctacggggg
     9241 tcgattccag gacctgttca tgggccagtg ggcaccccgc atcggcggcg ggaccgacca
     9301 ggtgcagcgg aacatcatcg gggagcgggt gctgggcctg cccggcgaca tccgggtcga
     9361 caaggacgtg gcgttccgcg acctgccccg ggcctgaacg aggccgctgc cgggcgcgta
     9421 gcgtggtggg gtgatcccgg ccgacgacac gacgcgcccg cgcccgggga ctgtcggacc
     9481 cgacgggccc accgtcgact tcgaccgggc ggcgacgacc ccgctgcggc ccgaggcccg
     9541 ggcggcgatg gcgccgttcc tggccggccg ctacggcaac ccgagctccg cgcacgccct
     9601 cggccgggac gcgatgcgag cggtggacga ggcgcgcgag cagctggccg acctgctggg
     9661 gtgcgcaccg agcgagctgg tgttcacctc gggcgggacc gaggccgacg tgcacgccgt
     9721 caccggcggg atccccaccc gggcggaccg cgtcgtgtgc tccgcggtcg agcacccggc
     9781 ggtgctggcc acggtgaccg ctctgggcgg atcgaccgtg gcggtgcacc gcgacggacg
     9841 gatcgacctc gacgcgctcg ccgccgagct gtcgtcgccg gggaaggtgg gcctggtgtc
     9901 ggtcatggcg gccaacaacg agatcggcac gctcaacgac ctggccgagg tcgccgcggt
     9961 cgtcgccgag cacgcgccgg gagctgcgct gcacaccgac gccgtgcagg ccgcggcgtg
    10021 gctcgacctg cgccgggtgg cgacgcccgc cgcgctggtg tcggtgtcgg cgcacaagtt
    10081 cg
//
LOCUS       DAOQOD010000113         9938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2474_length_9938_cov_11.041182, whole genome shotgun sequence.
ACCESSION   DAOQOD010000113 DAOQOD010000000
VERSION     DAOQOD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_9938_cov_11.041182"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLZ72_11130"
     CDS             <1..668
                     /locus_tag="PLZ72_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNA36893.1"
                     /translation="PAADEGTAPVTVTVSDPSALVGREVEVLVTEADRAKDRLVVSRR
                     DLQRRRRRQEEKDRLSSVKVGSRVTGRVVSVADYGAVVDLGGLRGLVHRSELAWERFG
                     APADVVSVGEQVTVEVIDVNRSKKRVSLSLKRTRPDPYGGLEVGQILPATVTRVVDYG
                     AFARLESGAEGLIHMSELSEVPGYRPDQLVVPGEELMVKVLNVDTAKHRIGLSVRRVL
                     VDD"
     gene            complement(697..1617)
                     /locus_tag="PLZ72_11135"
     CDS             complement(697..1617)
                     /locus_tag="PLZ72_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36894.1"
                     /translation="MTVEPTTESTPAASDTAPDPVAATTGLTVRYGTVDPDAVEMLGS
                     FPPEQDGPVWMVNLMRYRDRADYADGRDTELTGREADDLYAPLDVLADIGAQVVFFGD
                     VTDQLLGDGPPWDRVAVVKYPTHRSFLEMQTREDFPAHHVHKDAGMEATFIIAGQPFA
                     PPTWDGERPDPDRVPHPSTDEDGPVVVVHVVAFDRDSADTPEALATSIAHMDAYQAHA
                     ATVAVPHGVSIDGWFRAEATVVGDGRRWDQVRFNRFPSRAAFMAVVFDPSRLEVQHEH
                     REVAMSDTYTLIVRPQIDTLPASWEGLVDG"
     gene            complement(1614..3104)
                     /locus_tag="PLZ72_11140"
     CDS             complement(1614..3104)
                     /locus_tag="PLZ72_11140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNA36895.1"
                     /translation="MRRVGPAVLAVLVAAAGLVGCSDDDAAPQARSEPRPSAETTRPA
                     PTDGVDGGDGVGDPYFPQAGNSGYQVERYDLVMDVRIAGPDRLDATATITLVPTEDLR
                     SFHLDLLGFEVSKVTVDDADATFARDGREMVVTPERQLSEGDTTTVVVTYSGSPGRTG
                     SSSPTGGLIGDGGWVDLGDDWSTVIAEPIGAATWFPSNDHPSDKAIVNVTATVPAGLE
                     AVAGGRLVERTDAADRSTFRWEAAEPMSPYLASLTVGDMQLSEKDGPAGIRILDGVPA
                     RRPELLDGALSRFPEMITFFGQRFGPYPFRDAGNVIVPGLEPVALETQTRSVHSESIL
                     EPALGTLPDEVTAHELTHQWFGDAVGPADWSMIWLNEGFATYGEWLWLEHIGGRTVME
                     SARAAHDGDPDLNVPPATPGVGQLFGRTVYQRGGMFLVELGRLLGQPTFDQLLTTWVD
                     QHRFGVATTEQFVALAVEMAGPAKGAQVTALADAWLHAERIPELTP"
     gene            complement(3101..4369)
                     /locus_tag="PLZ72_11145"
     CDS             complement(3101..4369)
                     /locus_tag="PLZ72_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNA36896.1"
                     /translation="MSIRDVDLTPFADMAYWSHGSEVRADMFRQLRDEAPVLFMEEPE
                     IEGLPKGPGFWSVTRHADVVHVSRHADVFSSAQGTTIVDLPQELNEFMGSMINMDAPR
                     HTRLRQIVNKSFTPRMVNRIDEDVRVKARNILADVADTGGCDLVSRISAPLPLQIICE
                     MMGIPRDQWGRILELTNVVLGDQETVPDLPTLMAAVMEMAQIAREVGEDRLAEPRDDL
                     VSAMMHAEVDGEPLSPMEMASFFILLAAAGNETTRNAISHGVRLLTLHPDQRARWMAD
                     VDGVAPTAVEEIVRYATPVIQFRRTATRDTEIAGQPIGAGDKVVMWYESANRDERVFD
                     DPGTFDVTRTPNEHVGFGGGGPHFCLGANLARREIRVMFEELFRWLPDIVMTGEPDYL
                     QSGFIHGIKRMTCEFTPTEIPRLEPLGDTP"
     gene            4511..6262
                     /locus_tag="PLZ72_11150"
     CDS             4511..6262
                     /locus_tag="PLZ72_11150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA36897.1"
                     /translation="MSAPSAVRPTRIRLRPWQSEALERFADTSGPDFLAVATPGAGKT
                     TFALTAAVQDLADNPGRRLVVVAPTQHLKHQWSRAALRLGLHLDPDWSARDGELPGDM
                     HGIVTTYQQVATSSRVLAGLARGAFVVFDEIHHAGDDRAWGESVLEAFGGAARRLSLS
                     GTPFRSDTSAIPFVDYHLDEARADYEYGYGQALADRGVVRPVYFPRINGFMEWVAPDG
                     EVLSATFDDELAREQANQRLRTALSLEGQWLPAVLDQAQERLVELRRHHPEAGGLVIA
                     MDQAHAQDIARVLRERHGVRAVVATSDDPDASDHIASFAASADPWIVAVRMVSEGVDI
                     PRLRIAVFATTTTTELFFRQAVGRVVRWTPGLHGQGSRTQPAWFFLPDDPRLRRYSAE
                     LSESRRHSLRRRRDREELDSDRPDEIDAHDLGDDEQMSLFSVIGAVAIDGDDADGRGG
                     AAGSGPGGLGVFGDDPDDPGDRHAPEGVELVLLPPPLPGGGHVVVPADGGSGAGGSGP
                     SAGPMASMTRRERKARLRDQNADVAAELVRSTGWGYVQVQAELNRLTGIRKVSEATLE
                     QLESRLDHARRWLRRAG"
     gene            6252..>7193
                     /locus_tag="PLZ72_11155"
     CDS             6252..>7193
                     /locus_tag="PLZ72_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36898.1"
                     /translation="MPDDGGRLLDRRWVLRVALVLVALPFLLTFAKVATADDAPRNHD
                     VALIELRSGDVLGGDLPLVGSYERYGGNQPGPWLFWLLALPAAAGPLGIAAAVLLVGY
                     GAVAGMLAVAARRGGALLVCWTALLSMVLVLGRGLDRLADPWEPMLTVLLVALAVMAV
                     WEVASGTVSILPGVVALLWFLASVWTLLTSVAVAVGVVLVVAVAVVARDVVRSGDSGR
                     RRALLIAVGASVAVLVVMVTPTIVEQVVTTPGNLTQLLDAAGTSEPTVGLSGAWSALR
                     LQFDPWAPWLGRTLPVDLSSEIDGSAAGVVPVALLVWV"
     assembly_gap    7194..7293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7431..>9938)
                     /gene="hrpB"
                     /locus_tag="PLZ72_11160"
     CDS             complement(7431..>9938)
                     /gene="hrpB"
                     /locus_tag="PLZ72_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNA36899.1"
                     /translation="DEPWAAGGRVLVVQPRRLAARRAAARMAAVRGEPVGATCGYSVR
                     GDRRAGPTTRVELVTEGLALRRLQVDPALEGPERRVTAVLLDEFHERSLDVDLLLALL
                     IDIRSSLRPDLRLLVMSATLQPGPVAARLGDAAIGAVPVITATAPQHPVRTLHRPGRG
                     QDRIEDRVATVIHESLDAGPESDGALGDVLVFLPGRGEIRRTAERLSRGVGAEVVVHQ
                     LHGSVPAAEQDAALAPDPRGRRRVVLATAIAETSITVDGVRIVIDSGRRRTLRVHAAT
                     GLPGLTTTPVSLAGADQRRGRAGRTGPGTCYRLWSAEEERHRATADPPEILESDLAGL
                     LLQVLAWGAHPGDLPFLDAPTGPHLTAAGVLLADLGATDSTDPDTTRLTARGRALAQL
                     GFHPRLGAVVHAALDGPLGRQRTAELAALLEVDPPGDPDLVERLLRMSRGERSRELAD
                     ATRDWRNRLPRDRGRHAEQDDPDGIGSAEAIGAAASSAILAGYRDRLARRRDGDRTDG
                     RGRQAVIYQLVGGGEVALQPPDHPLSRSRWLAVASLDRGDTAMGRTAAGPADRGRIHL
                     AVAVDDSAAEAALRDRTADVDEVFWDGSRREVRAVRRRQVGSITVDERPWRDPPSDAL
                     SAALADGVRIAGAGRVFDRWPEAEELRARLALSRPTPDATGSLAAATAAGGSASGDPT
                     DDGRADDDVDILVARAASTGRVSRAELATLDVARWLLDGLSWTDRTALEEWTPLHLDL
                     LSGRRVRVRYGAEGPVVSTRLQDLLGTDTHPTVGIERVPVTVELLSPAGRPLQRTSDL
                     PGFWRGSYAAVRAEMRGRYPKHRWPERPWEPIPGPRS"
BASE COUNT         1240 a         3415 c         3730 g         1453 t
ORIGIN      
        1 ctccggcagc cgacgagggg accgcccctg tgacggtgac cgtctcggac ccgtccgccc
       61 tggtcggacg tgaggtcgag gtgctggtca ccgaggccga ccgggccaag gaccgtctgg
      121 tcgtgtcccg ccgtgacctg cagcggcgtc gccgtcgcca ggaggagaag gaccggctgt
      181 cgtcggtgaa ggtcggcagc cgggtgaccg ggcgggtcgt gtcggtcgcg gactacggcg
      241 ccgtggtcga cctgggcggg ctgcgcggtc tggtgcaccg cagcgagctg gcatgggagc
      301 ggttcggagc accggccgac gtcgtgtcgg tcggcgagca ggtcaccgtc gaggtgatcg
      361 acgtcaaccg ctccaagaag cgggtgtccc tgtcgctcaa gcgcacccgt cccgacccct
      421 acggcgggct ggaggtcggt cagatcctgc cggcgacggt cacccgggtg gtcgactacg
      481 gggcgttcgc ccggctcgag tccggtgccg agggcctgat ccacatgtcg gagctgtcgg
      541 aggttccggg ctaccgaccc gaccagctcg tggtccccgg cgaggagctc atggtcaagg
      601 tcctcaacgt cgacacggcc aagcaccgca taggtctgtc ggtccgccgg gtcctggtcg
      661 acgactgatc gtcgcccgtg ctcccgtcgg gtcgtctcac ccgtcgacga gcccttccca
      721 ggacgcgggc agggtgtcga tctgcggtcg gacgatcagc gtgtaggtgt cgctcatcgc
      781 gacctctcgg tgctcgtgct ggacctcgag gcgggagggg tcgaacacca ccgccatgaa
      841 ggcggcccgc gacgggaacc ggttgaagcg gacctggtcc cagcggcgac cgtcgccgac
      901 gacggtggcc tcggcccgga accagccgtc gatgctcacg ccgtggggca ccgcgacggt
      961 ggccgcgtgg gcctggtagg cgtccatgtg ggcgatgctg gtcgccagcg cctcgggcgt
     1021 gtcggccgag tctcggtcga aggcgacgac gtgcacgacc accaccggac cgtcctcgtc
     1081 ggtcgagggg tgcggcaccc ggtcggggtc ggggcgctcg ccgtcccagg tgggcggggc
     1141 gaagggctgg ccggcgatga tgaaggtcgc ctccatcccg gcgtccttgt ggacgtggtg
     1201 ggcggggaag tcctcgcggg tctgcatctc caggaaggac cggtgggtcg ggtacttcac
     1261 cacggccacg cgatcccacg gtggtccgtc gccgagcagc tgatcggtca cgtcgccgaa
     1321 gaacacgacc tgcgcaccga tgtcggcgag gacgtcgagc ggggcgtaca ggtcgtcggc
     1381 ctcccgtccg gtcaactcgg tgtcacggcc gtcggcgtag tcggcccggt cccggtagcg
     1441 catcaggttg accatccaca ccggtccgtc ctgttccggt gggaacgagc ccagcatctc
     1501 gaccgcgtcg gggtcgacgg tgccgtagcg gacggtcaga cccgtcgttg ccgcgaccgg
     1561 gtccggcgcg gtgtcggagg ccgcgggggt ggactcggtc gtgggttcga ctgtcatggg
     1621 gtcagctccg ggatccgctc ggcgtgcaac caggcgtcgg ccagcgccgt cacctgggcg
     1681 cccttggccg gccccgccat ctcgacggcc agcgccacga actgctcggt ggtggccacc
     1741 ccgaagcggt gctggtccac ccatgtggtg aggagctggt cgaaggtggg ctggccgagc
     1801 agccggccga gctcgaccag gaacatcccg ccgcgctggt agacggtcct gccgaacagc
     1861 tggccgacgc ccggagtcgc gggcggtacg ttcaggtcgg ggtcgccgtc gtgggcggcg
     1921 cgggcggact ccatgacggt gcgcccgccg atgtgctcca gccacagcca ctcgccgtag
     1981 gtggcgaagc cctcgttcag ccagatcatc gaccagtcgg ccggaccgac cgcgtcgccg
     2041 aaccactggt gggtgagctc gtgggcggtg acctcgtcgg ggagggtgcc gagggccggc
     2101 tcgaggatcg actcggagtg caccgatcgg gtctgggtct cgagcgcgac gggttccagg
     2161 ccggggacga tgacgttgcc ggcgtcgcgg aacgggtacg gcccgaagcg ctggccgaag
     2221 aacgtgatca tctcggggaa gcgggacagc gcgccgtcga gcagctccgg tcgtcgggcc
     2281 gggaccccgt cgaggatcct gatgccggcc ggtccgtcct tctccgacag ctgcatgtcg
     2341 ccgacggtga ggctggcgag gtacggcgac atcggctcgg ccgcctccca gcggaacgtg
     2401 gagcggtccg cggcgtcggt gcgctcgacc agtcgaccgc cggcgacggc ctccaacccc
     2461 gcgggtacgg tcgcggtgac gttgacgatc gccttgtccg acgggtggtc gttggatggg
     2521 aaccaggtgg ccgcaccgat cggctcggcg atcaccgtcg accagtcgtc gccgaggtcg
     2581 acccacccgc cgtcgccgat gagccccccg gtgggcgacg agctgccggt ccggcccggc
     2641 gaccccgagt acgtcacgac cacggtcgtg gtgtcgccct cggagagctg gcgttcgggg
     2701 gtgacgacca tctcgcggcc gtcgcgggcg aacgtggcgt cagcgtcgtc gacggtcacc
     2761 ttcgacacct cgaagcccag caggtccagg tggaagctgc ggaggtcctc ggtgggcacc
     2821 agcgtgatcg tggcggtggc gtcgagtcgg tcggggccgg cgatgcgcac gtccatgacc
     2881 aggtcgtagc gctcgacctg gtagcccgag ttgccggcct gtgggaagta cgggtcgccg
     2941 accccgtcgc cgccgtcgac cccgtcggtc ggggccggcc gggtggtctc ggcgctgggg
     3001 cggggctccg agcgggcctg gggcgccgcg tcgtcgtcgg agcagccgac gagacccgcg
     3061 gcggcgacca gcaccgccag cactgccgga ccgacccgcc tcacggcgtg tcgccgagcg
     3121 gttcgaggcg ggggatctcg gtcggggtga actcgcaggt catccgcttg atgccgtgga
     3181 tgaagccgga ctgcaggtag tcgggctcgc cggtcatcac gatgtcgggc agccaccgga
     3241 acagctcctc gaacatgacc cggatctcgc gtcgggccag gttggcgccc aggcagaagt
     3301 gcggtccgcc gccgccgaag ccgacgtgct cgttgggggt ccgggtcacg tcgaaggtgc
     3361 ccgggtcgtc gaagacgcgc tcgtcgcggt tggccgactc gtaccacatg accaccttgt
     3421 cgccggcgcc gatcggctgt ccggcgatct cggtgtcgcg ggtggcggtg cggcggaact
     3481 ggatgaccgg ggtcgcgtag cggacgatct cctcgaccgc ggtgggggcg accccgtcga
     3541 cgtcggccat ccagcgggcc cgctggtcgg ggtgcagggt cagcagccgg accccgtggc
     3601 tgatcgcgtt gcgggtggtc tcgttgccgg cggcggcgag caggatgaag aagctcgcca
     3661 tctccatcgg cgacagcggc tcgccgtcga cctcggcgtg catcatcgcc gacaccaggt
     3721 cgtcgcgggg ttccgccaga cggtcctcac cgacctcccg ggcgatctgg gccatctcca
     3781 tgaccgccgc catgagggtc ggaaggtccg ggaccgtctc ctggtcgccg agcacgacgt
     3841 tggtgagctc caggatccgt ccccactggt cgcgggggat gcccatcatc tcgcagatga
     3901 tctgcagcgg cagcggggcc gagatccggc tgaccaggtc gcaaccgccg gtgtcggcga
     3961 catcggcgag gatgttgcgg gccttcaccc tcacgtcctc gtcgatgcgg ttgaccatcc
     4021 ggggcgtgaa gctcttgttg acgatctggc gcagccgggt gtggcgcggt gcgtccatgt
     4081 tgatcatcga tcccatgaac tcgttgagct cctgggggag gtcgacgatc gtcgtgccct
     4141 gcgccgagct gaacacgtcg gcgtggcgcg acacgtggac cacgtccgcg tggcgggtga
     4201 ccgaccagaa acccggcccc ttggggagcc cctcgatctc gggttcctcc atgaacagca
     4261 ccggcgcctc gtcgcgcagc tggcggaaca tgtcggcgcg gacttccgag ccgtgggacc
     4321 agtacgccat gtcggcgaac ggagtcaggt cgacgtcgcg gatgctcatg tgtccccctg
     4381 gtcggccgtc gcacgccggc ggacgggtcg gcgacgacgg gtgtcgggtc ggtgtccggg
     4441 gcgtcgggcg gtgctcctaa gctagcgagc ccatgccggt gcccacgttc gaaaccgacg
     4501 atccgcgccg gtgagcgccc cgtccgccgt ccgccccacc cggatccgcc tgcgtccctg
     4561 gcagtccgag gccctggagc gcttcgccga cacctccggc cccgacttcc tggcggtggc
     4621 cacccccggg gccggcaaga ccaccttcgc cctgaccgcc gcggtgcagg acctggccga
     4681 caaccccggg cgacgcctcg tcgtggtcgc accgacccag cacctcaagc accagtggtc
     4741 gcgtgccgcg ctgcgcctcg gtctgcacct cgacccggac tggtcggccc gcgacgggga
     4801 gctgcccggc gacatgcacg ggatcgtgac cacctaccag caggtcgcca cctcgtcccg
     4861 ggtgctggcc gggctggcgc ggggagcgtt cgtcgtcttc gacgagatcc atcacgccgg
     4921 tgacgaccgg gcctgggggg agtcggtgct ggaggccttc ggcggcgcgg cccgccgcct
     4981 gtcgctgtcg ggcacgccgt tccgctccga cacctccgcc atcccgttcg tcgactacca
     5041 cctcgacgag gcccgcgccg actacgagta cggctacggc caggcgctgg ccgaccgcgg
     5101 ggtcgtgcgg ccggtgtact tcccgcgcat caacgggttc atggagtggg tcgcccccga
     5161 cggcgaggtg ctgtcggcca ccttcgacga cgagctggcc cgggagcagg ccaaccagcg
     5221 gttgcgcacc gcgctgtcgc tggagggcca gtggctgccg gccgtgctcg accaggccca
     5281 ggagcgcctg gtcgagctgc gccgccacca ccccgaggcg ggcggcctgg tgatcgccat
     5341 ggaccaggcc cacgcgcagg acatcgcccg ggtgctccgt gaacgtcacg gcgtccgggc
     5401 cgtggtcgcc acctccgacg accccgacgc gtccgatcac atcgcgtcgt tcgcggcgtc
     5461 ggccgacccg tggatcgtgg cggtgcgcat ggtctccgag ggtgtcgaca tccctcggct
     5521 gcgcatcgcg gtgttcgcca cgaccaccac caccgagctg ttcttccgtc aggccgtcgg
     5581 gagggtcgtg cgctggaccc cgggtctgca cggtcagggg tcgcggaccc agccggcctg
     5641 gttcttcctg ccggacgacc ctcgtctgcg tcgctactcc gccgagctga gcgagtcccg
     5701 acgtcactcc ctgcgccgtc gccgggaccg tgaggagctc gactcggacc gcccggacga
     5761 gatcgacgcc cacgacctcg gcgacgacga gcagatgagc ctgttctcgg tgatcggggc
     5821 ggtcgcgatc gacggcgacg acgccgatgg ccgcggggga gcggccgggt ccgggccggg
     5881 cgggctcggc gtgttcggcg acgatcccga cgaccccggt gaccgtcacg ctcccgaggg
     5941 ggtggagctg gtgctgctgc caccgccgct gcccggcggc ggtcacgtgg tggtcccggc
     6001 cgacgggggt tccggcgcag ggggttcggg cccgtccgcc ggtccgatgg cgtcgatgac
     6061 ccgacgcgag cgcaaggccc ggctccggga ccagaatgcc gacgtcgcgg cggagctggt
     6121 gcgatcgacc gggtggggat acgtgcaggt gcaggccgaa ctgaaccgac tgaccgggat
     6181 ccgcaaggtg tccgaggcca cgctcgaaca gctcgagtcc cgtctcgacc acgcccgtcg
     6241 gtggctccgt cgtgccggat gacggcggcc gtctgctcga tcgccgttgg gtgctgcgcg
     6301 tcgccctggt cctggtcgcc ctgccgttcc tcctgacctt cgccaaggtc gccaccgcgg
     6361 acgacgcccc ccgcaaccac gacgtcgccc tgatcgagct ccgttccggc gacgtcctcg
     6421 gcggggacct tccgctggtc ggctcctacg agcgctacgg cggcaaccag ccgggtccgt
     6481 ggctcttctg gctcctcgcc ctgcccgcgg cggccggccc gctcgggatc gcggcggcgg
     6541 tcctgctggt cggatacggg gccgtggccg ggatgctggc ggtggcggcc cgtcgcggag
     6601 gcgccctgct ggtgtgctgg acggcgctgc tgtccatggt gctggtcctg ggccgcggcc
     6661 tcgatcgtct ggccgatccg tgggagccga tgctcaccgt cctgctggtc gcgctggccg
     6721 tcatggccgt gtgggaggtc gcgtccggga cggtgtcgat cctgccgggg gtggtggcgc
     6781 tgctgtggtt cctggcgtcg gtgtggaccc tgctgacatc ggtggcggtg gccgtcgggg
     6841 tggtgctggt ggtcgcggtc gcggtcgtgg cccgtgacgt cgtccgctcc ggtgacagcg
     6901 gccgtcggcg cgccctgctg atcgcggtcg gggccagcgt ggcggtgctc gtggtcatgg
     6961 tgaccccgac gatcgtcgag caggtggtga ccacaccggg gaacctgacc cagctgctgg
     7021 acgccgccgg cacgtcggag ccgaccgtcg gcctgtcggg ggcgtggagc gctctgcgtc
     7081 tccagttcga cccgtgggcg ccgtggttgg gtcggaccct gccggtcgac ctgtcgagcg
     7141 agatcgacgg gtccgcggcg ggtgtcgtac cggtcgcgct cctggtgtgg gtcnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgcgtt ctgacgacaa gtcacgacac
     7321 tggcggtact ccagtgtcgt cagaaacttg gcccgtcgcg ttctgacgac aagtcacgac
     7381 actggcggta ctccagtgtc gtcagaaact cggatcgttg gagcgcgggg tcaggagcgt
     7441 gggccgggga tcggctccca gggtcgctct ggccaccggt gcttggggta cctgcccctc
     7501 atctcggcgc ggaccgcggc gtagctgccg cgccagaacc ccggcagatc agaggtgcgc
     7561 tgcagcggac gcccggcggg tgacagcagc tcgacggtga cggggacccg ctcgatgccc
     7621 acagtggggt gggtgtcggt gccgagcagg tcctgaagcc gggtcgacac gacgggtcct
     7681 tccgctccgt agcgcacccg tacccgacga cccgagagca ggtcgaggtg caggggtgtc
     7741 cactcctcca gcgccgtccg gtccgtccag ctcagcccgt ccaggagcca ccgggcgacg
     7801 tcgagcgtcg ccagctccgc tcgggagacc cgtccggtgg acgcggcacg ggcgacgagg
     7861 atgtcgacgt cgtcgtcagc gcgcccgtcg tcggtgggat cgccggaagc ggacccacct
     7921 gcggcagtcg cggcggccag actgccagtc gcgtcggggg tgggtctgct cagcgccagg
     7981 cgggcacgga gctcttccgc ctcgggccaa cggtcgaaca cccggcccgc tccggcgatc
     8041 cggaccccgt cggccagagc ggcactcagc gcatcggagg gcgggtcgcg ccacggacgc
     8101 tcgtcaacgg tgatggaccc gacctgccga cgacggacgg ctcggacctc gcgtcggctg
     8161 ccatcccaga acacctcgtc cacatcagcg gtccggtccc ggagagcggc ctcggcagct
     8221 gagtcgtcca ccgcgacggc caggtggatg cgaccgcggt cggcgggtcc ggcggccgtg
     8281 cggcccatcg ctgtgtcgcc gcggtcgagg gacgcgaccg ccagccatcg cgaccggctg
     8341 agcgggtggt ccggtggttg cagggcgacc tctccgccgc ccacgagctg gtagatcacc
     8401 gcctgccggc cccgaccgtc ggttcggtcg ccgtcgcggc gtcgggccag ccggtcgcga
     8461 tagccggcga ggatcgctga gctcgcggcc gcgccgatcg cctcggcgga gccgatgccg
     8521 tcggggtcgt cctgctccgc gtgccggccc cgatcgcggg gcagacggtt gcgccagtcc
     8581 cgtgtcgcgt cggcgagctc cctggaacgc tcaccgcggg acatccggag gagtcgctcg
     8641 acgaggtcgg gatcccccgg cgggtcgacc tcgagcaggg cggccagctc cgcggtccgc
     8701 tgccgaccca gcggtccgtc gagcgcggcg tggaccacgg cgccgagccg ggggtggaac
     8761 ccgagctggg ccagggcacg gccccgggcg gtgagtcggg tcgtgtccgg gtcggtgctg
     8821 tcggtggccc cgagatcggc gagcaggacc ccggccgcgg tcaggtgggg gccggtcgga
     8881 gcgtcgagga agggcaggtc cccgggatgg gcgccccacg cgaggacctg caggagcaga
     8941 cccgccaggt ccgactccag gatctcgggt ggatcggcgg tggcccggtg ccgttcctcc
     9001 tctgcggacc acagccggta gcaggtgccc ggtccggtcc ggccggcccg accacgcctc
     9061 tggtcggcac cggcgagcga gaccggtgtg gtggtcaagc cgggcagacc ggtcgcggcg
     9121 tggacgcgca gggttcggcg tcggccggag tcgatgacga tgcgcacccc gtcgacggtg
     9181 atcgacgtct cggcgatcgc ggtggccagc accacccgac ggcggccccg cgggtccggt
     9241 gccagcgctg cgtcctgctc cgcggcgggg accgagccgt gcagctggtg gacgacgacc
     9301 tcggccccga ccccgcggga gaggcgctcg gcggtgcggc ggatctcgcc acggccgggg
     9361 aggaagacca gcacgtcgcc gagcgccccg tcggactcgg gtccggcgtc caacgactcg
     9421 tggatcaccg tcgccacccg gtcctcgatg cggtcctgac cccggccggg gcggtgcagg
     9481 gttcgcaccg ggtgctgcgg ggcggtggcg gtgatgacgg ggaccgctcc gatcgcggca
     9541 tcgccgagcc gagcggcgac gggtcccggc tggagggtgg cggacatgac cagcagtcgg
     9601 aggtcgggcc gcaaggagga gcggatgtcg atcagcaacg ccagcagcag gtccacgtcg
     9661 agggagcgtt cgtggaactc gtcgaggagc actgcggtca cccgtcgctc cggcccctcg
     9721 agcgcggggt cgacctgcag ccgtcggagg gcgagccctt cggtgaccag ctcgacccgg
     9781 gtcgtggggc cggctcggcg gtcgccgcgg accgagtacc cgcacgtcgc gccgacgggc
     9841 tcaccccgca ccgcggccat gcgggccgcg gcgcgtcggg cggccagacg gcggggttga
     9901 acgacgagga cgcggcctcc ggcggcccac ggctcgtc
//
LOCUS       DAOQOD010000114         9932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2477_length_9932_cov_10.971145, whole genome shotgun sequence.
ACCESSION   DAOQOD010000114 DAOQOD010000000
VERSION     DAOQOD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_9932_cov_10.971145"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1944
                     /gene="ilvD"
                     /locus_tag="PLZ72_11165"
     CDS             76..1944
                     /gene="ilvD"
                     /locus_tag="PLZ72_11165"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNA36900.1"
                     /translation="MPQYRSHTSTHGRNMAGARALWRATGMTDDDFGKPIIAIANSFT
                     QFVPGHVHLKDMGQLVAGEVTKAGGVAKEFNTIAVDDGIAMGHAGMLYSLPSRDLIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGKTRLAGEEV
                     PVNLISAILSSGDDSVSDDEVAQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GTLVATHSDREQLFLESGRLVVELARRYYDCDDDSVLPRSIADLRAFTNAMSLDIAMG
                     GSTNTVLHLLAAAQEGEVPFTMADIDALSRRMPNICKLAPMSDFFHVEDCHRAGGIPA
                     ILGELDRAGLIDAAAPTVHSPTMADALDRWDVARPTCTDEARTFWSAGPAGIPTQTAF
                     SQSTRWPSLDLDRAEGCIRDVEHAYSAEGGLAVLFGNIATDGCIVKTAGVAEEIWHFD
                     GTARVYESQDAAVAAILGGEVVAGDVVVIRYEGPRGGPGMQEMLYPTSYLKSRGLGME
                     CALLTDGRFSGGTSGLSIGHVSPEAAEGGLIGLVRTGDPIRIDIPARSIHLDVDDAEL
                     ARRHEAEHERGDAAWTPHSRDRTVSTALQVYAAFTTSAARGAVRDLSQMAPTHRPD"
     gene            complement(1969..2106)
                     /locus_tag="PLZ72_11170"
     CDS             complement(1969..2106)
                     /locus_tag="PLZ72_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36901.1"
                     /translation="MSGWWAAVGVGALLAAVAVREVLRWRGGRWFGGMRSGSRPDDEE
                     S"
     gene            complement(2103..2618)
                     /locus_tag="PLZ72_11175"
     CDS             complement(2103..2618)
                     /locus_tag="PLZ72_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36902.1"
                     /translation="MAIGVYPGSFDPLTIAHLAVADAAFDTLGLDRLDLAISGTALGK
                     EHLDDTSVQRRLDGIRDATADRPWLEVVAVREQLIVDIAEGYDVVVMGADKWTQVIDP
                     VWYDDEDARDAALARLPRVAVAPRAGHRVPPELELAVPEGLEHVSASAVRAGRTGWAA
                     PTPTAVERSHR"
     gene            2645..3175
                     /locus_tag="PLZ72_11180"
     CDS             2645..3175
                     /locus_tag="PLZ72_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNA36903.1"
                     /translation="MTGTGPQADVEGDDERVEVVDRSGRVLDVVGRSQMRAERLRHRC
                     TFVVVRNSGGEVLVHRRSPDKDMWPNRWDLAVGGVVRAGEDWDDAAARELAEEVGVLG
                     VDLVALSGDLAYDDADVSELARIWTVSWDGPVTFDDGEVVATEWVPLDELGRLLGRRS
                     FVADSVALVWPLIDST"
     gene            3238..3747
                     /locus_tag="PLZ72_11185"
     CDS             3238..3747
                     /locus_tag="PLZ72_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36904.1"
                     /translation="MALSSPRNPVRRPLAVLSIALAGVALVAVGCGDDSADSSSSTTA
                     SSTTVSTAPTSTSSTTSSGAEVGDGRIQVTIGGEQRTLTIEACTSGDSSSMELTAHDD
                     SGNQMMVKVAGGQGSVTYRGSSEDREGAIRSVDIQADGTFDLGGILSVADDSAPGPDD
                     LTVTGLCPA"
     gene            3987..5717
                     /locus_tag="PLZ72_11190"
                     /pseudo
     CDS             3987..5717
                     /locus_tag="PLZ72_11190"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            5744..6280
                     /gene="ilvN"
                     /locus_tag="PLZ72_11195"
     CDS             5744..6280
                     /gene="ilvN"
                     /locus_tag="PLZ72_11195"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNA36905.1"
                     /translation="MNVPSTTNGSATNAVPRHHVLSVLVENKAGVLARVSSLFARRGY
                     NIYSLAVAPTDEDRFSRITVVVDVESAPLEQIVKQLHKLINVIKISELDPRDSLEREL
                     LLCTVKATPEVRGQVIELVDLFEGRVLDVGHDEMTLSLDADPSRLDDLEELLRPFGII
                     DLQRTGRVALARLEHVAP"
     gene            complement(6352..7119)
                     /locus_tag="PLZ72_11200"
     CDS             complement(6352..7119)
                     /locus_tag="PLZ72_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36906.1"
                     /translation="MNHTPTPRRRAGALLVAVLASFAFVAASCSSGSDESVSKESDTK
                     ETTTTEKERSGGGIASGILDRAAKSGKFSSSGFGGDVEECIGGSVIDSLGEDEAQTMA
                     DTDPSEYSPDQVDALVAAFNDCVPGEVIAPTLVSSFYEGMGAAEPSDSTMVDCVADAV
                     DGQTGDIVREGINAQENDSTPELTLQAFDQCMPPEDITALLTEAFTSSGLTAEQATCV
                     ATALEGQISVSELAAAGQNDGSPELEAKVKAAAEGCG"
     gene            7209..8636
                     /locus_tag="PLZ72_11205"
     CDS             7209..8636
                     /locus_tag="PLZ72_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA36907.1"
                     /translation="MEHARQFYIGGTWVEPRSSDTLPVIDPATEDVIETIAMGGPDDV
                     DAAVAAARSAFDGFAATTVQERAALLDRVIEAYQARMGDLADVVRAEMGAPTSLAQTA
                     QVPAALAHLTTARALIEDFPFEEESGSTLVVREPVGVCALITPWNWPLNQIACKVAPA
                     LAAGCTMVLKPSEVAPLDALIFAEILDEAGVPAGVFNLVNGNGLDVGQPLASHPEVDM
                     VSFTGSTRAGTAVAAAAAPSVKRVAQELGGKSANIVLDDADLDAVVRRDVTRMCTNSG
                     QSCNAPSRMLVPRDLLARAAEVAADAAGSIRVGVPADEATTVGPVVSQTQYDRIQALI
                     EKGIGEGATLVAGGPGRPDGLDVGYYVRPTVFTDVTNDMTIAQEEIFGPVLVMIGYDD
                     DDDAVRIANDTRYGLSGYVSGSQDRARDIARRIRTGNVHVNGAGPDFDAPFGGYKQSG
                     NGREWGRLGFEEFLETKAIMGWTGR"
     gene            8900..9709
                     /locus_tag="PLZ72_11210"
     CDS             8900..9709
                     /locus_tag="PLZ72_11210"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNA36908.1"
                     /translation="MTIEAGGVTRTAVLDVPAGAAGTAVEASAAPRPLLVSLHPFAMD
                     PAIWDSYSALATAGTARGYVVVTPLGSAPGPRWAVPGGLAADVDDLAFISALVDHIGR
                     VTCVDRNRVFAVGFSAGAAMAQALSCTMPDRFAAVAGSGGVNLTSLCPGAPPTDVLVL
                     HGTADPIVPPAGSQVPFSPPVGLAVAAVVAVDAARAGCDPVPELDRPEPSVLHERYRG
                     CADGRRVESLTLIGAGHTWAGSPNPLLELVAGPTNTDISATATVLDFFGSG"
BASE COUNT         1438 a         3523 c         3538 g         1433 t
ORIGIN      
        1 ggtggggcgg gaacgggggg ggggcgggag agcggggggg gggctggggc tgaggggccg
       61 cagtagcctc gccacgtgcc ccagtaccga tcccacacct ccacccacgg ccgcaacatg
      121 gccggcgccc gcgccctgtg gcgggcgacg gggatgaccg acgacgactt cggcaagccg
      181 atcatcgcca tcgccaactc gttcacccag ttcgtgccgg gccacgtgca cctcaaggac
      241 atgggccagc tcgtggccgg cgaggtgacc aaggccggcg gcgtggccaa ggagttcaac
      301 acgatcgcgg tcgacgacgg catcgccatg ggccacgccg gcatgctcta cagcctcccg
      361 agccgggacc tgatcgcgga ctcggtcgag tacatggtca acgcccactg cgcggacgcg
      421 ctggtgtgca tctccaactg cgacaagatc acccccggca tgctcatggc cgccatgcgg
      481 ctgaacatcc cgacggtgtt cgtgtcgggc gggccgatgg aggcgggcaa gacccggctg
      541 gccggcgagg aggtgccggt caacctgatc tccgccatcc tgagctccgg cgacgacagc
      601 gtcagcgacg acgaggtcgc ccagatcgaa cgctctgcgt gtccgacctg cgggtcgtgc
      661 tcgggcatgt tcaccgccaa ctcgatgaac tgcctgaccg aggcgctcgg gctcagcctg
      721 ccgggcaacg gcacgctggt cgccacccac tccgaccgcg agcagctgtt cctcgagtcc
      781 gggcggctcg tcgtcgagct cgcccgccgg tactacgact gcgacgacga ctcggtgctg
      841 ccccggtcga tcgctgacct ccgggcgttc accaacgcca tgtcgctcga catcgccatg
      901 ggcggcagca ccaacaccgt cctgcacctg ctcgccgccg cccaggaggg cgaggtcccc
      961 ttcaccatgg ccgacatcga cgcgctgagc cggcggatgc ccaacatctg caagctcgcc
     1021 ccgatgtcgg acttcttcca cgtcgaggac tgccaccgcg ccggaggcat ccccgccatc
     1081 ctcggcgagc tcgaccgggc cggactgatc gacgccgccg cgccgacggt gcactcgccg
     1141 accatggccg acgccctcga ccgctgggac gtcgcccgtc cgacctgcac cgacgaggcc
     1201 cgcacgttct ggtcggccgg ccccgccggc atccccaccc agaccgcgtt cagccagtcg
     1261 acgcgctggc cgagcctgga cctggaccgt gccgagggct gcatccgtga cgtcgagcac
     1321 gcctactccg ccgagggcgg gctcgccgtg ctgttcggca acatcgccac cgacggctgc
     1381 atcgtcaaga ccgcgggtgt ggccgaggag atctggcact tcgacggcac cgcccgggtc
     1441 tacgagagcc aggacgcggc ggtggcggcc atcctcggcg gcgaggtcgt cgccggcgac
     1501 gtcgtcgtca tccgctacga gggcccgagg ggcggtccgg gcatgcagga gatgctgtac
     1561 ccgacgagct acctcaagag ccgggggctg gggatggagt gcgccctgct caccgacggc
     1621 cgcttctccg ggggcacgtc cggactgtcg atcggccacg tctcacccga ggcggccgag
     1681 ggcggcctca tcggcctggt ccgcaccggc gacccgatcc gcatcgacat cccggcccgc
     1741 agcatccacc tcgacgtcga cgacgccgag ctggcccggc gtcacgaggc cgagcacgag
     1801 cggggcgacg cggcgtggac accccacagc cgggaccgca cggtgtcgac ggcgttgcag
     1861 gtctacgcgg cgttcacgac ctcggcggcc cggggcgcgg tgcgcgacct cagccagatg
     1921 gcgccgaccc accggcccga ctgagcacga catgggaacg gtccgggcct agctctcctc
     1981 gtcgtcgggg cggctgccgg agcgcatccc cccgaaccag cggccgcccc gccagcgcag
     2041 aacctcgcgc acggccaccg cggccaggag tgccccgacc ccgacagccg cccaccagcc
     2101 gctcatcggt ggctcctctc cactgctgtc ggtgtcggcg ccgcccatcc ggtccgaccg
     2161 gcgcgcaccg cgctggccga cacgtgctcc aggccctccg gcaccgccaa ctccagctcc
     2221 ggcggcaccc gatggccggc ccggggcgcc accgcgaccc gcggcagtcg ggccagcgcg
     2281 gcgtcccggg cgtcctcgtc gtcgtaccag accggatcga tcacctgggt ccacttgtcg
     2341 gcacccatga ccaccacgtc gtagccctcg gcgatgtcga cgatgagctg ctcccgcacg
     2401 gcgacgacct ccagccaggg ccggtccgcc gtggcgtcgc ggatgccgtc gagtcgccgc
     2461 tgcaccgagg tgtcgtcgag gtgctccttg ccgagagcag tgccggagat ggcgaggtcg
     2521 agccggtcga ggccgagcgt gtcgaacgcg gcgtcggcca ccgcgaggtg agcgatcgtg
     2581 agcgggtcga aggagccggg gtacacgccg atggccacct ccggacggta ccgtcgccga
     2641 ggtggtgacc ggaaccggac cccaggcgga cgtcgaaggc gacgacgagc gggtcgaggt
     2701 ggtggaccgg tccgggcggg tcctcgacgt cgtcgggcgg tcgcagatgc gcgccgagcg
     2761 tctccgtcac cggtgcacgt tcgtggtggt ccgcaacagc gggggagagg tgctcgtgca
     2821 ccgacggagt cccgacaagg acatgtggcc gaaccgatgg gacctggcgg tcggcggggt
     2881 ggtgcgggcc ggagaggact gggacgacgc ggcggcacgc gagctggccg aggaggtcgg
     2941 cgtcctcggt gtcgatctgg tggcgctgag tggggacctc gcctacgacg acgcggacgt
     3001 gtcggaactc gcccggatct ggacggtgag ctgggacggc cctgtcacct tcgacgacgg
     3061 cgaggtggtc gctacggagt gggtcccact cgatgagctg ggcaggctgc tcgggcgacg
     3121 ctcgttcgtg gccgactcgg tcgccctggt gtggccgctg atcgacagca cctgatcggc
     3181 tgcacctgat ggacgccgcg ccggatgcga tgccaggtcg gatgtagggt cgccgcgatg
     3241 gcactcagct cacctcgcaa tccggtccgt cgcccgctcg ccgtgctctc gatcgctctg
     3301 gccggcgtgg ctctggtggc ggtcggctgc ggcgacgact cggccgactc gtcgtcgtcc
     3361 accaccgcca gctcgacgac cgtgtcgacc gcacccacgt cgaccagctc caccacctcg
     3421 tcgggcgcgg aggtcggcga cggtcgcatc caggtgacca tcggtggtga gcagcggacg
     3481 ctgaccatcg aggcttgcac cagcggcgac agctcgtcga tggagctcac cgcccacgac
     3541 gactcgggca accagatgat ggtgaaggtg gccggaggcc agggcagcgt gacctaccgg
     3601 gggtcgagcg aggaccggga gggtgcgatc cgttcggtcg acatccaggc cgacgggacc
     3661 ttcgacctcg gcgggatcct ctcggtggcc gacgacagcg ctccgggccc tgacgacctg
     3721 accgtcaccg ggctctgccc cgcctgaccc cggtcggtgt cgggcaaggg gttggcgaag
     3781 gggggagcgc cgcggcagac tggcggggat gcagcagtcg gaccctcgtc gcagcctcgt
     3841 ggtggtactc acctaggcgt cacgaccggg accctgccgc gcgtgcgcca cgacctcccg
     3901 cctcgggggg tcgttccggt tttcaggcca gacccacagc caggacagac ccagacacgg
     3961 acttccacac ggatggaccc agcagcatga acaccaacgg cggatccgcc ctcatcaagg
     4021 ccctcgagct cgaaggcgtc gaggtgatct tcggtcttcc cggcggggcg atcctgccgg
     4081 tgtacgaccc gatcctcgac agcccgatcc gtcacgtgct cgtgcgccac gagcagggcg
     4141 cgggtcacat ggccgagggg tacgcgcacg ccaccggccg acccggcgtg gccatggtga
     4201 cctcgggtcc cggcgccacc aacatcgtca cgccgatctg cgacgcgttc atggactcgg
     4261 tccccatggt cgtcatcacc ggccaggtcc cccgcccggc gatcggcacc gacgccttcc
     4321 aggagtgcga catcgtcggc atcacccggt cgatcaccaa gcacaacgag ctggtcaccc
     4381 ggcccgagga catccccctg gccatccgcc aggcgttcca catcgccacg accggccggc
     4441 ccggcccggt gctcgtcgac atccccaagg acatcgtcga tcccaagaac ccggcgtcgg
     4501 ccatggactg ggactggccc tccgacgcgg atgtcgcgtc gagcctgccc ggctacaagc
     4561 cgaccaccaa gggccacccc cgcaagatca aggaggcggt cgagctgatc ctgggcgcgc
     4621 agcggccggt catctacgcc ggcggcggaa tcctcggtgc ccgggcagcc gaggtgctcc
     4681 gtgagctggc cgagatgacc gacatcccgg tggtcaccac cctgatggcc cggggcgcct
     4741 tccccgacga ccacccgctg tgcctgggca tgcccggcat gcacggcaac tacaccgcgg
     4801 tcaccgccat gcagcgcgcc gacctgctca tcgctctcgg cagccgcttc gacgaccgcg
     4861 tcaccggcaa ggtcgacgcc ttcgcccccg acgcacggat catccacgtc gacatcgacc
     4921 cggccgagct cggaaaggtg cgtcgccccg acgtgccgat cgtcggcgac tgccgcctgg
     4981 tgatcgagga gatggtccgg ctgctgaacc accgcaccga cggcgacgtg gcggcccacc
     5041 aacccgaccg cagcgcgtgg aagcaggcga tctcgggctg gcaggagaag ttcccgatgg
     5101 tctacgagcc gtcggtcgac ggcggaccgc tcaagcccca gtacgtgctc gaggagctgc
     5161 gcgaccgcgc cccggaggac tgcatcgtcg ccagcggcgt cggccagcac cagatgtggg
     5221 ccagccagta ctggaagttc aaccacccct acacctggat caactcgggc ggggcgggca
     5281 cgatggggtt ctcggtcccg gccgccatcg gcgccaaggt cggccgaccc gaccggatgg
     5341 tgtgggcggt cgacggcgac ggctgcttcc agatgaccgc ccaggagctg gtcactgcgg
     5401 cgaccgagcg catcccggtc aaggtcgcga tcctcaacaa cgcgtacctg ggcatggtgc
     5461 gccagtggca ggagatgttc tacgaggagc gctactccga ggtgtacctg tcgcccgacc
     5521 tgcccgacta cgtgaagtgg tccgaggcga tgggttgcgt ggccatgcgg gtcgagtcgc
     5581 ccgaggaggt cgggccggcc atcgacaagg ccaacgagat cgacgaccga cccgtcgtgc
     5641 tcgagttccg caccgacagc gccgagaagg tgttcccgat ggttccggcg ggcaagagca
     5701 acgacgagat cgtggtggac ccgtcgctgc gggagggaga ccgatgaacg ttccgagcac
     5761 gaccaacgga tccgcgacca acgccgtgcc ccgccaccac gtgctgtcgg tgctggtcga
     5821 gaacaaggcc ggtgtcctgg cccgggtctc gagcctgttc gcccggcggg gctacaacat
     5881 ctactcgctg gcggtggcgc cgaccgacga ggaccgcttc agccgcatca ccgtcgtggt
     5941 cgacgtggag agcgctccgt tggagcagat cgtcaagcag ctccacaagc tcatcaacgt
     6001 gatcaagatc agcgagctcg acccccgcga ctcgctggag cgcgagctgc tgctgtgcac
     6061 ggtcaaggcc acccccgagg tgcgcggcca ggtgatcgag ctggtcgacc tgttcgaggg
     6121 ccgggtgctc gacgtcggcc acgacgagat gaccctcagc ctggacgcgg acccgagccg
     6181 cctcgacgac ctcgaggagc tgctgcggcc gttcgggatc atcgacctgc agcgcacggg
     6241 ccgggtggcg ttggcgcgtc tggagcacgt cgctccctga tcgcgcctgg acgcgccacg
     6301 cgggccccgg agggcccgcg tggagaggat cctcgaccgg gaggtcgggg gtcagccgca
     6361 gccctcggct gccgccttga ccttcgcctc gagctccggt gagccgtcgt tctgcccggc
     6421 cgcggccagc tcgctgaccg agatctgacc ctcgagcgcg gtggccacgc aggtggcctg
     6481 ctcggcggtc agcccggagc tggtgaacgc ctcggtgagc agcgcggtga tgtcctctgg
     6541 cggcatgcac tggtcgaacg cctgcaacgt gagctcgggg gtggagtcgt tctcctgggc
     6601 gttgatgccc tcgcggacga tgtcgccggt ctggccgtcg acggcgtccg cgacgcagtc
     6661 gaccatggtc gagtcgctcg gctcggcggc acccatgccc tcgtagaacg acgacaccag
     6721 ggtcggggcg atgacctcac ccgggacgca gtcgttgaac gcggcgacca gcgcgtcgac
     6781 ctggtccggt gagtactccg acggatcggt gtcggccatc gtctgggcct cgtcctcgcc
     6841 gaggctgtcg atgaccgaac cgccgatgca ctcctcgacg tcgccaccga agccgctgga
     6901 gctgaacttc ccgctcttgg cggcacggtc gaggatcccc gaggcgatgc cgcctccgga
     6961 gcgctccttc tcggtggtgg tcgtctcctt ggtgtcggac tccttggaga cggactcgtc
     7021 gctgccgctg ctgcacgacg cggcgacgaa ggcgaacgac gccaggacgg cgacgagcag
     7081 ggcgccggct cgccgacggg gggtgggggt gtggttcatg gcgttcaggc tactggtccg
     7141 ctgtccgaac gcggaccacc cgaagggtgg caggcttcac gggtcgaccg accgcgggag
     7201 gatccgccat ggagcacgca cggcagttct acatcggggg cacgtgggtg gaaccccgct
     7261 cgagcgacac cctcccggtg atcgacccgg cgaccgagga cgtcatcgag acgatcgcga
     7321 tggggggacc cgacgacgtg gacgccgcgg tggcggcggc ccgctccgcc ttcgacgggt
     7381 tcgcggccac gaccgtgcag gagcgtgctg ccctgctcga ccgggtgatc gaggcgtacc
     7441 aggctcgcat gggcgacctg gccgacgtcg tccgggccga gatgggcgcg ccgacgtcgc
     7501 tggcgcagac cgctcaggtg cccgccgcgc tcgcgcacct caccacggcc cgggccctga
     7561 tcgaggactt cccgttcgag gaggagtcgg gcagcacgct ggtcgtccgc gagccggtcg
     7621 gcgtctgcgc gctgatcacg ccgtggaact ggccgctcaa ccagatcgcc tgcaaggtcg
     7681 ccccggcgct ggccgccggc tgcacgatgg tgctcaagcc gtccgaggtc gcaccgctcg
     7741 acgccctgat cttcgccgag atcctcgacg aggccggggt gccggccggg gtgttcaacc
     7801 tggtcaacgg caacgggctc gacgtcggac aaccgctggc gtcgcacccg gaggtcgaca
     7861 tggtctcgtt caccggctcg acgcgggccg gcaccgcggt ggccgccgcg gcggcgccgt
     7921 cggtcaagcg ggtcgcccag gagctcggcg gcaagtcggc caacatcgtc ctcgacgacg
     7981 cggatctcga cgccgtggtc cgacgtgacg tcacccggat gtgcaccaac tccggccagt
     8041 cctgcaatgc gcccagccgc atgctggtgc cccgggacct gctggcccgg gcggccgagg
     8101 tggccgccga cgccgccggc tcgatccgcg tcggtgtccc cgccgatgag gcgacgaccg
     8161 tcggaccggt ggtgtcgcag acgcagtacg accggatcca ggcgctgatc gagaagggga
     8221 tcggcgaggg cgccacgctg gtggccgggg gcccggggcg acccgacggt ctggacgtcg
     8281 gctactacgt ccggcccacg gtgttcaccg acgtcaccaa cgacatgacg atcgcccagg
     8341 aggagatctt cggtccggtg ctggtgatga tcggatacga cgacgatgac gacgccgtgc
     8401 gcatcgccaa cgacacccgc tacggcctgt ccggctacgt ctccggctcc caggaccggg
     8461 cgcgggacat cgcccgtcgg atccgcaccg gcaacgtcca cgtcaacggc gccggtcccg
     8521 acttcgacgc cccgttcggc ggctacaagc agtcgggcaa cggccgcgag tggggccgcc
     8581 ttggtttcga ggagttcctc gagaccaagg ccatcatggg ctggaccggc cgctgaaccc
     8641 ctcccgctct ccctcctcac ccacggaagg tccgcgcagg gtcgtgttct cggttcgacc
     8701 gagagctggc ctcctccggt cggtactcgt cgccgcgatc gtcggagcgg tcgtcgtacc
     8761 cgcgtgcact ccgccgacgg tccccgatcg gggcgtggtc accgctccga tcggtggggg
     8821 tcgggaaccg tcggtcgacg tggccccgtc ggccggctgc tcgggagtcg tctctccgat
     8881 cgctcccggg cgacgtaccg tgacgatcga ggccggaggt gtcacccgta ccgcggtcct
     8941 cgacgttccc gccggcgcgg ctggcaccgc ggtcgaggcc tcggccgcgc cgcgaccgct
     9001 gctggtcagc ctgcacccgt tcgcgatgga ccccgccatc tgggactcgt actcggctct
     9061 ggccacggcg gggaccgccc ggggatacgt cgtggtcacg ccgctcggca gcgctcccgg
     9121 tccccggtgg gcggtgcccg gaggactggc cgccgacgtc gacgacctgg ccttcatatc
     9181 cgcgctggtc gaccacatcg gtcgggtgac ctgcgtcgat cggaaccggg tgttcgcggt
     9241 gggcttctct gccggtgcgg ccatggccca ggccctgtcg tgcacgatgc ccgaccgctt
     9301 cgcggcggtg gccgggtcgg gtggggtgaa cctcacgtcg ctgtgtcctg gcgctcctcc
     9361 caccgatgtc ctggtcctgc acggaaccgc tgacccgatc gtgccgccgg ccggttcgca
     9421 ggtcccgttc tccccgccgg tcggactggc ggtggccgcc gtcgtggccg tcgacgcggc
     9481 ccgcgccggg tgcgatcccg tgccggagct cgaccgaccg gagccgtcgg ttctgcacga
     9541 gaggtaccgg ggatgtgccg acggtcgacg ggtggagagc ctgacgctga tcggggcggg
     9601 acacacgtgg gccggatcgc cgaacccgct gctcgagctg gtcgccggtc cgaccaacac
     9661 cgacatctcg gcgacggcga ccgtgctgga cttcttcggc agcggctgac gcggtgttgc
     9721 ccgcgctggg gcgcgggtgg tgtgagtcgg ggggggggtg tgggtggggt gtgcccggcg
     9781 ggtcccgcgc tcaggcggga gcgggtctgg gtgggggtgg tggttggtgg tgttgggtgg
     9841 gtagttgggt gccgtcggtg gtggtgatgg tgaagtatga accaccctgg gttcgttgga
     9901 ggctcctgag cttggaaggc tggagtcatg cc
//
LOCUS       DAOQOD010000115         9845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2504_length_9845_cov_13.300613, whole genome shotgun sequence.
ACCESSION   DAOQOD010000115 DAOQOD010000000
VERSION     DAOQOD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2504_length_9845_cov_13.300613"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLZ72_11215"
     CDS             complement(<1..782)
                     /locus_tag="PLZ72_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36909.1"
                     /translation="MAEVVIIHGAFHELWGPFRLWVRWTPSVLDGLWNAGVPVQDVSL
                     SRLQERSQVAFWGDLVRPEPRSADQQPDDDAADDGTVGPTPDLADLLDGHPAVAGADG
                     AGLDALVQQVARDTNARSMELLARYVLDPDMKSRVHERLERHLDSDTRVVIAHSMGTV
                     IAYQVLCTRPDLQVDLITLGSPLGDQAMVFDTLEPAPVDGRGVWPAAVRRWTNVAAEG
                     DLATAACPRLADRFGERVEDHLVFNGRHAHNVEPYLNSAPTGA"
     gene            complement(839..1798)
                     /gene="speB"
                     /locus_tag="PLZ72_11220"
     CDS             complement(839..1798)
                     /gene="speB"
                     /locus_tag="PLZ72_11220"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF002564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNA36910.1"
                     /translation="MSETNGPGGRDDGDLGGAGAAGMTWAGPTTFARRPLTRDLSGVD
                     IAVVGVPFDNATTNRPGARFGPRAIREMSTLSGEYPDGLWPWEVDLFATHRVVDYADV
                     AFAPGYTDQMLENVERAAGDVIDAGASVLGLGGDHLVAYPLLRAAAERFGPLSLIHLD
                     AHSDTWDVGDELNHGTMFLRAAREGVVDPTTSAQVGMRTPNESHGFNVIHADEMFRLG
                     IDATVDRIREVVGGRAAYLTVDIDFLDPAYAPGTGTPVVGGPTTWQARQILFGLRGLD
                     VVGADLVEVAPAYDPSGQITALAGATLAHDELHLLAEARDRRR"
     gene            complement(1827..>2615)
                     /locus_tag="PLZ72_11225"
     CDS             complement(1827..>2615)
                     /locus_tag="PLZ72_11225"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNA36911.1"
                     /translation="RIDPVWNPAAAEFVAAANAVSLLMPAVEPYFAGSIRRALPDLEQ
                     TLARIARTFVRQELEHQRQHRRFNGRLIERFPALARPEARSRWIYGWLSRTRSLRFSL
                     AFAAASETIAYSLARWTSDHLAEFLRGADPAATDLFVWHLAEEVEHKSVAFDVWAALD
                     GSRWRYARAGALSIALLAWLVLWSIALQMHSEHRLRSPATWWRTCRLLVSFAFEVVPN
                     LLVSAVPGHHPDQFCDPDWYRHWLVDLEQRRPVASPDPAGRRAS"
     assembly_gap    2616..2715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2716..3541
                     /locus_tag="PLZ72_11230"
     CDS             <2716..3541
                     /locus_tag="PLZ72_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNA36912.1"
                     /translation="TMSNKALRITAIAAVAVLGLGLAACSSDDDSSSTTTEAGGGGDS
                     KLVICSDIPYAPMEMEATDTKTPSGYTGFDIDLVQAIATGADKTLEVKVTPFDGIFAA
                     MDAGTCDAVVSSVSITDERKQNMDFSEPYFDAEQSVMVTKANAEKYKTLADLTGQEIG
                     VQSGTTGEEYVNSHKPSGSTVKALPGASDLFAALQSGTIAAVVQDYPINAYRTTQDKN
                     FVITEKIPTEEQYGMAVKKGNTETLDLLNKGLSTAKSDGTYDKLYEKYFGEKPPTS"
     gene            3654..4457
                     /locus_tag="PLZ72_11235"
     CDS             3654..4457
                     /locus_tag="PLZ72_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNA36913.1"
                     /translation="MTRSQRSNLIQWAVFVAGLALVALVLGVTVRWGDLADRFFKWED
                     FTDQFPGIVTEAARNTLVYTAIGYLGGLVIGLVVALMRQSQVPPARVIASVYIEVFRA
                     LPALLTILIVGFGLPIAFGAENLPGFLAQPWGAGCLALALVAGAYLAETIRAGIEAVP
                     RGQVEAARSLGMPTGVTMRSIVLPQAFRVILPPLTNELVLLLKDTSLLAVLGVAIGQR
                     ELTTWGRTISNAIGNYTPLIAAAVCYLVITLPLTFLARWVERRTQADRR"
     gene            4454..5233
                     /locus_tag="PLZ72_11240"
     CDS             4454..5233
                     /locus_tag="PLZ72_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNA36914.1"
                     /translation="MSGGSTIDPAGSGAARPTVDIRGLCKSFGSNDVLKGVDFHVDQR
                     EVVVVLGPSGSGKSTLLRCVNLLETPDSGQVLIDGIDICDEDTDVNAVRRRIGMVFQQ
                     FNLFAHLSALDNVTIAQRKVLKRSAAEAQEVARRNLERVGVGDKAGHFPAQLSGGQQQ
                     RVAIARALAMDPEIMLFDEATSALDPELVGDVLKVMRSLAEDGMTMMVVTHEIGFARN
                     VADRVVFMADGVVVEEGPPAQVIDDPQHPRTRQFLRQVHDR"
     gene            complement(5279..6712)
                     /locus_tag="PLZ72_11245"
     CDS             complement(5279..6712)
                     /locus_tag="PLZ72_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36915.1"
                     /translation="MARKHLLAITAVAVLALIASACGASGGDKQAEGGGDGATTTAAQ
                     EAAAGSFGDLPAPVCGPGKDKVAEGEGGTGTDKLYIGVATDRSSSIRPGLNKEMWDAS
                     EAFTKWCNEAGGIGGLKIEPVELDAALLDVTKAMTAGCHSVFAMVGGGWAQDNLQFSG
                     KDSSDFHKCGLIDIPGYAVSPEKADSSGQVQPVPNPGTSVSNTFIRDYAKLYPKESKK
                     VAIAYAELPALEVVKEKYKAAAKDVGLDVVAELPYSAVGMTDWTPLAQSIINSGAGTL
                     MWVGEAGNATSALAKLKEQGWKGHALLETNMYDPLLFSQGNEAVEGSVVRQTVHPIEE
                     ADQWPATRQYLDMLKKYVPDAKVAPLGIQSMSAWLLFATAADACAKANGGTISRQCVL
                     EQAAAQKDWTGGGLHAAQDPAPFSDAKSSSCSMLLVVKDGKFERLYPEEIGGTDDDSE
                     GFHCPSDGVTDVPANAGKGVVEPGAKI"
     gene            complement(6824..8062)
                     /locus_tag="PLZ72_11250"
     CDS             complement(6824..8062)
                     /locus_tag="PLZ72_11250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA36916.1"
                     /translation="MRLGDIVNADALGAGKPLDGIRVLALEQMQALPYATQLLARLGA
                     DVVKIESPRGGDMGRSSLPAMTDPEGRQVGATFLRNNLSKRSICIDLKSADGRELVLE
                     LAPRFDVVAENFKAGGLARLGLGYDDVAAVHPAVIYLSVSGFGNTTDSPYKDRPAFAA
                     IVEAMSGIYDYQARDDRPPQASPVGALGDISAALFGAIGVLAALRQRDRTGDGQYVDV
                     AMLDALVAMTDIVSNFWSMGLTERAPLILENFRASDGWFVLQVGREPQFEALAELIGH
                     PEWLTDERLATRPGWVAHMETVIRPGIESWAAPLTRQEAAAALAAAGLASGPCLTDDE
                     VVHDPHVAMRDMLVEMPRTDGVDQPILTPGNPVKISGVAEGPESRVPWLGEHTDAVLG
                     EELGLSADRLAALRAAGVIA"
     gene            8134..8316
                     /locus_tag="PLZ72_11255"
     CDS             8134..8316
                     /locus_tag="PLZ72_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36917.1"
                     /translation="MGSSGQKPRKPNQRHLPKVGTKANVDYEIAQKRAEVFRGWPWII
                     VAVLLVVLLIAFIAIT"
     gene            complement(8309..>9845)
                     /locus_tag="PLZ72_11260"
     CDS             complement(8309..>9845)
                     /locus_tag="PLZ72_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNA36918.1"
                     /translation="VEEDAPVGLDRADLAEVLRRRSLLHDEMRPEAVARRRRQGRHTV
                     RSLVEQLLDTDSFSEYGGLAVAAQRGRREIDDLIERTPADGLLTGTGTIDGVAVAVIG
                     YDATVLAGTQGFVNHRKTDRILAVVERHGLPVVLFAEGGGGRPGDVDAPVVTGLDVPT
                     FARFARLSGRVPTVAVVSGYCFAGNAALVGCCDVIVATEDSNLGMAGPAMIEGGGLGR
                     VEPTAIGPIEVQVANGTVDVRVEDDITAVRAARALVGLGSTRVDTAIGPEDQTGLRDA
                     VPAARKRLYDVRSILATVTDPGSLVELRSGHAPGMVTALGRIAGRAVGVLANDPAHLG
                     GAIDAEGAASATRLVHLCQRWDLPVVSFCDTPGFMVGPDAEAAGQVRPFGDLFVAAAS
                     LDTPWITVILRKAYGLGAQAMAAGGMHEPVLTVAWPTGELGGMGLEGAVRLGFARELD
                     AIEDPAERQEREAALIELAYLRGGALNVASHIEIDDVIDPADTRRRILDALTAAGRGD
                     AVR"
BASE COUNT         1427 a         3369 c         3490 g         1459 t
ORIGIN      
        1 gccccggtcg gcgcgctgtt caggtagggc tcgacgttgt gggcgtggcg gccgttgaac
       61 accaggtggt cctcgacccg ctcgccgaac cggtcggcca ggcggggaca cgccgcggtg
      121 gccaggtctc cctcggcggc gacgttggtc cagcggcgca cagcggccgg ccacacgccc
      181 cgaccgtcga ccggtgcggg ctcgagcgtg tcgaacacca tcgcctgatc gcccagcggt
      241 gatcccaggg tgatcaggtc gacctgcaga tcgggccgtg tgcaaaggac ctggtaggcg
      301 atcacggtcc ccatcgagtg agcgatgacc acgcgggtgt cggaatcgag atgccgctcc
      361 aggcgctcgt ggacacgcga cttcatgtcg gggtcgagga cgtaccgggc cagcagctcc
      421 atcgatcggg cgttggtgtc acgtgcgacc tgctgcacga gagcgtcgag gccggctccg
      481 tccgcgccgg ccaccgccgg gtgcccgtcg agcaggtcgg ccaggtcagg ggtcggtcca
      541 acggtgccgt cgtcggccgc gtcgtcgtcc ggttgctggt cggcggaccg cggctcgggt
      601 cgcacgagat caccccagaa cgcgacctgg gaccgctcct ggagccgact cagcgacacg
      661 tcctgcaccg gaaccccggc gttccagagc ccgtcgagca cgctcggcgt ccagcgcacc
      721 cacagccgga acggtcccca cagctcgtgg aacgccccgt ggatgatcac gacctcggcc
      781 atgcccggag tgtggcacct cccggggtgc tggcgccgga ccccgcggga cgatcgcgtc
      841 aacgacgccg gtcgcgggcc tcggccagca ggtgcagctc gtcgtgggcg agggtggcgc
      901 cggccagggc ggtgatctga ccgctcgggt cgtaggcggg cgcgacctcg accaggtcgg
      961 caccgacgac gtcgaggccg cgcagaccga acaggatctg tcgggcctgc cacgtcgtcg
     1021 gtccaccgac gaccggggtg ccggtcccgg gcgcgtaggc gggatcgagg aagtcgatgt
     1081 cgacggtcag gtaggcggca cggcccccga ccacctcccg gatccggtcg accgtggcgt
     1141 cgatgccgag gcggaacatc tcgtccgcgt ggatcacgtt gaacccgtgg ctctcgttgg
     1201 gggtgcgcat gccgacctga gccgacgtgg tcgggtcgac gacgccttcg cgggcggccc
     1261 gcaggaacat cgtcccgtgg ttgagctcgt cgccgacgtc ccaggtgtcg gagtgggcgt
     1321 cgaggtggat cagcgacagc ggaccgaacc gttcggccgc ggcgcgcagg agcgggtacg
     1381 ccaccaggtg gtcaccaccg agtccgagga cactcgcgcc ggcgtcgatg acgtcccctg
     1441 cggcgcgttc gacgttctcc agcatctgat ctgtgtatcc gggagcgaac gccacgtccg
     1501 cgtagtcgac gacgcgatgg gtcgcgaaca ggtcgacctc ccacggccac aacccgtcgg
     1561 ggtactcgcc ggagagggtc gacatctccc ggatggcccg gggtccgaat cgggcgccgg
     1621 gtcggttggt ggtcgcgttg tcgaacggga ctcccaccac ggcgatgtcg acaccgctca
     1681 ggtcccgcgt gagagggcgc cgggcgaagg tggtcgggcc cgcccaggtc atcccggcgg
     1741 cgcccgcccc tcccaggtcg ccgtcgtcac ggccaccggg gccgttggtc tcgctcaccg
     1801 acacaggttc ccacaccgca gtcggatcac gacgcccgtc gtccggctgg gtcaggcgac
     1861 gcgaccggcc gacgctgctc gaggtcgacc agccagtggc gataccagtc ggggtcgcag
     1921 aactggtcgg ggtggtggcc cggcacggcc gacacgagga ggttggggac gacctcgaac
     1981 gcgaagctga ccagcagccg acaggtgcgc caccaggtgg cgggggatcg gaggcgatgc
     2041 tcggagtgca tctggagcgc gatcgaccag agcacgagcc aggccaggag ggcgatcgac
     2101 aaagcgccgg cgcgggcgta gcgccaccgg gacccgtcga gcgccgccca cacgtcgaag
     2161 gcgaccgact tgtgctcgac ctcctcggcc aggtgccaga cgaacaggtc ggtggcggcg
     2221 gggtccgcgc cccgcaggaa ctcggcgagg tggtcgctgg tccagcgagc gagggagtag
     2281 gcgatggtct cggaggcggc cgcgaaggcc aggctgaacc gcaggctccg ggtgcgcgac
     2341 aaccagccgt agatccaacg ggaccgcgcc tcgggtcggg ccagggcggg gaagcgctcg
     2401 atcagccgtc cgttgaaccg ccggtgctgg cgttggtgct cgagctcctg gcggacgaag
     2461 gtccgggcga tccgggcaag ggtctgctcg aggtcgggca gtgcccgtcg gatggaaccg
     2521 gcgaagtacg gctcgacagc gggcatcagg agcgacacgg cgttggcggc ggccacgaac
     2581 tcggccgcgg ccgggttcca caccgggtcg atccgnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnncacga tgtccaacaa ggcgctccga atcaccgcca tcgctgcggt
     2761 cgcggtcctg ggtctcggcc tggccgcgtg cagcagcgac gacgacagct cgtccaccac
     2821 gaccgaggcc ggcgggggcg gcgactccaa gctggtcatc tgctcggaca tcccgtacgc
     2881 cccgatggag atggaggcga ccgacaccaa gacgccgagc ggctacaccg gcttcgacat
     2941 cgacctggtg caggcgatcg ccaccggggc cgacaagacc ctcgaggtca aggtcacgcc
     3001 gttcgacggc atcttcgccg ccatggacgc cgggacctgc gacgcggtgg tctcctcggt
     3061 gtcgatcacc gacgagcgca agcagaacat ggacttctcg gagccctact tcgatgccga
     3121 gcagtcggtg atggtgacca aggccaacgc cgagaagtac aagacgctgg ccgatctgac
     3181 cggccaggag atcggggtcc agtccggcac caccggcgag gagtacgtca acagccacaa
     3241 gccgagcggc tcgaccgtca aggcgctccc cggggcgtcg gacctgttcg ccgccctcca
     3301 gtccggaacc atcgctgcgg tcgtgcagga ctacccgatc aacgcctacc gcacgaccca
     3361 ggacaagaac ttcgtcatca ccgagaagat cccgaccgag gagcagtacg ggatggcggt
     3421 gaagaagggc aacaccgaga cgctcgacct gctgaacaag ggtctgtcga cggccaagtc
     3481 cgacggcacc tacgacaagc tgtacgagaa gtacttcggc gagaagccgc ccacgtcgtg
     3541 accacctgcg gccggttcgg gctcggcccg ccaccgagcc gcgggatctg acacgctgac
     3601 gggccgggac ggttcgacca cgatggtcga gcctcccggc ccgttccgcc accatgaccc
     3661 gttcgcaacg ctccaacctg atccagtggg ccgtgttcgt ggccggcctc gctctggtcg
     3721 cgctcgtgct gggggtgacg gtccgctggg gtgacctcgc cgaccgcttc ttcaagtggg
     3781 aggacttcac cgaccagttc ccggggatcg tcaccgaggc ggcccgcaac acccttgtgt
     3841 acaccgcgat cggctatctc ggtggcctgg tcatcggtct ggtagtggcg ctcatgcgcc
     3901 agtcgcaggt gccgccggcc cgggtgatcg catcggtcta catcgaggtg ttccgggcgt
     3961 tgccggcact gctgacgatc ctgatcgtgg gcttcggact gccgatcgcc ttcggggccg
     4021 agaacctccc gggcttcctg gcccagccgt ggggcgcggg gtgccttgcc ctggcgctgg
     4081 tggccggcgc gtatctggcc gagacgatcc gggccggcat cgaggccgtt ccccgtggtc
     4141 aggtcgaggc tgctcgctcc ctgggcatgc cgaccggggt cacgatgcgt tcgatcgtcc
     4201 tgccccaggc gttccgggtg atcctgccgc cgttgaccaa cgagctggtg ctgctgctca
     4261 aggacacctc gctgctggcc gtgctcggcg tggcgatcgg tcagcgggag ctgaccacct
     4321 gggggcgcac catctcgaac gccatcggca actacacccc gctgatcgcg gcggcggtct
     4381 gctatctggt gatcaccctg ccgctgacgt tcctggcccg ctgggtcgag cgtcggaccc
     4441 aggcggatcg tcgatgagcg ggggatcgac catcgacccc gctgggtccg gagcagcacg
     4501 gcccaccgtc gacatccgtg ggctctgcaa gagcttcggg tccaacgacg tgctcaaggg
     4561 cgtcgacttc cacgtggacc agcgggaggt cgtggtggtg ctcggaccgt cgggatcggg
     4621 caagtccacc ctgctgcgct gcgtgaacct gctcgagacc ccggactccg gccaggtgct
     4681 gatcgacggc atcgacatct gcgacgagga caccgacgtc aacgcggtgc gtcgccggat
     4741 cgggatggtg ttccagcagt tcaacctgtt cgcccacctc agcgccctcg acaacgtcac
     4801 catcgcccag cgcaaggtcc tgaagcgctc ggccgccgag gcccaggagg tcgctcgccg
     4861 caacctggag cgcgtcggcg tcggcgacaa ggccggccac ttcccggccc agctctcggg
     4921 cggtcagcag cagcgggtgg cgatcgcccg ggcgctggcg atggacccgg agatcatgct
     4981 gttcgacgag gcgacctcgg cgctcgatcc ggagctcgtg ggcgacgtgc tgaaggtcat
     5041 gcgctcgctg gccgaggacg gcatgaccat gatggtggtc acccacgaga tcggcttcgc
     5101 ccgcaacgtg gcggaccggg tggtgttcat ggccgacggc gtcgtggtcg aggagggtcc
     5161 cccggcacag gtgatcgacg accctcagca cccgcggaca cgccagttcc tacgccaggt
     5221 ccacgaccgc tgaggtgatc cgggtgccgt cccggacggg cacggcaccc ggatcaggtc
     5281 agatcttcgc ccccggctcc acgacgccct tgccggcgtt ggcggggacg tcggtcaccc
     5341 cgtcgctcgg gcagtggaag ccctcgctgt cgtcgtcggt cccaccgatc tcctcggggt
     5401 agaggcgctc gaacttgccg tccttgacga cgaggagcat cgagcacgag ctcgacttgg
     5461 cgtcgctgaa gggggcgggg tcctgagcgg cgtggagtcc accgccggtc cagtccttct
     5521 gggccgcggc ctgctccagg acgcactgcc gggagatcgt gccgccgttg gccttcgcgc
     5581 aggcgtcggc ggcggtggcg aagagcagcc aggcgctcat cgactggatc ccgaggggag
     5641 cgaccttggc gtcggggacg tacttcttca gcatgtccag gtactgtcgg gtcgccggcc
     5701 actggtcggc ctcctcgatc gggtgcacgg tctggcgcac gacgctgccc tcgacggcct
     5761 cgttgccctg cgagaacagc agtgggtcgt acatgttggt ctcgagcagg gcgtgaccct
     5821 tccaaccctg ctccttgagc ttggccaggg cgctggtggc gttgccggcc tcaccgaccc
     5881 acatgagcgt cccagcaccg gagttgatga tcgactgcgc cagcggcgtc cagtcggtca
     5941 tcccgaccgc cgaatagggg agctcggcca ccacgtcgag cccgacgtcc ttcgccgcgg
     6001 ccttgtactt ctccttcacc acctcgagcg ccgggagctc ggcgtaggcg atggcgacct
     6061 tcttggactc cttcgggtac agcttggcgt agtcacggat gaaggtgttc gacaccgatg
     6121 tgcccgggtt gggcaccggt tggacctggc cgctcgagtc ggccttctcc ggtgacactg
     6181 catagcccgg gatgtcgatc agcccgcact tgtggaagtc ggagctgtcc ttgccgctga
     6241 actggaggtt gtcctgggcc cagcctcccc cgaccatggc gaacaccgag tggcagcccg
     6301 cggtcatggc cttggtcacg tcgagcaggg cggcgtcgag ctccaccggt tcgatcttca
     6361 ggccgccgat gccgccggcc tcgttgcacc acttggtgaa ggcctccgac gcgtcccaca
     6421 tctccttgtt gagcccgggg cggatcgacg agctgcggtc ggtggcgacg ccgatgtaga
     6481 gcttgtcggt gccggtccca ccctcgccct cggcgacctt gtccttgccg ggcccgcaca
     6541 ccggggccgg gaggtcgccg aaggacccgg cggcggcctc ctgtgcggcg gtggtggtgg
     6601 ctccgtcacc gccgccctcg gcctgcttgt cgccaccgct ggcaccgcat gccgatgcga
     6661 tcagggcgag gaccgcgacc gcggtgatcg ccagaaggtg cttccgagcc atgctgttcc
     6721 ccctggtgct ccggcgcgga cccgccgctc cgagtggaca gccgcacggt agcccgctgt
     6781 gaccccggac acatggcatc cgcggggtgc gcccgggtgg atgtcaggcg atgacgccgg
     6841 cggcgcgcag cgctgccagt cgatccgccg acaggccgag ctcctcgccg agcacggcgt
     6901 cggtgtgctc gccgagccac gggacgcggc tctcgggccc ctcggccacg ccggagatct
     6961 tcaccgggtt gcccggcgtg aggatcggct ggtcgacccc gtcggtccgc ggcatctcca
     7021 ccagcatgtc gcgcatggcg acgtgcgggt cgtggacgac ctcgtcgtcg gtcaggcacg
     7081 gacccgacgc cagcccggcc gcggcgagcg ccgcggcggc ctcctggcgg gtcagcggtg
     7141 cggcccagga ttcgatgccg ggccggatca ccgtctccat gtgagcaacc catccgggac
     7201 gggtcgccag ccgttcgtcg gtcagccact cggggtgtcc gatcagctcc gccagtgcct
     7261 cgaactgcgg ctcacgtccg acctgcagga cgaaccaccc gtcggacgcc ctgaagttct
     7321 cgaggatcag cggcgcccgc tcggtgaggc ccatcgacca gaagttgctg acgatgtcgg
     7381 tcatggcgac cagtgcgtcg agcatggcga cgtcgacgta ctggccgtcg ccggtgcggt
     7441 ctcgctggcg gagcgcggcg aggacgccga tggcaccgaa cagggcggcg gagatgtcgc
     7501 cgagagcccc gaccggactc gcctggggcg gccggtcgtc gcgggcctgg tagtcgtaga
     7561 tgcccgacat ggcctcgacg atggcggcga aggccggacg gtccttgtac ggggagtcgg
     7621 tggtgttgcc gaagccggag accgacaggt agatgaccgc ggggtgaacg gcggcgacgt
     7681 cgtcgtagcc gagccccagt cgggcaagtc cgccggcctt gaagttctcg gccacgacgt
     7741 cgaagcgggg ggccagctcc aggaccagct ctcggccgtc ggcggacttg aggtcgatgc
     7801 agatgctgcg cttggacagg ttgttgcgca ggaacgtcgc gccgacctgc cgtccctcgg
     7861 gatccgtcat ggccggcagc gacgaccgtc ccatgtcacc ccctcggggt gactcgatct
     7921 tgaccacgtc cgccccgagc cgggccagca gctgggtggc gtagggcagg gcctgcatct
     7981 gctccagggc caggacccgg atcccgtcga ggggctttcc ggccccgagg gcatcggcgt
     8041 tgacgatgtc tccgagtcgc atgggtgcga ccgtagtggg atctgacggg tcgtcacatc
     8101 ttcgaccgtc ggccctgggc tagcgtcggg gccgtgggaa gcagcggcca gaagccccgg
     8161 aagccgaacc agcgccacct gcccaaggtg ggcaccaagg cgaacgtcga ctacgagatc
     8221 gcccagaagc gggccgaggt gttccgcggc tggccctgga tcatcgtggc cgtgctgctg
     8281 gtcgtcctgc tgatcgcgtt catcgccatc acctgaccgc gtcgccgcgt cccgccgcgg
     8341 tgagcgcgtc gaggatccgt cgcctcgtgt cggcggggtc gatcacgtcg tcgatctcga
     8401 tgtgcgacgc cacgttgagc gccccgcccc ggaggtaggc cagctcgatc agggccgcct
     8461 ccctctcctg acgctcggcc gggtcctcga tggcgtcgag ctcgcgggcg aagcccaacc
     8521 ggaccgcgcc ctcgagcccc atgccgccga gctcaccggt gggccacgcc acggtcagga
     8581 ccggttcgtg cattccccct gcggccatcg cctgcgcgcc caagccgtac gccttgcgca
     8641 ggatcacggt gatccacggg gtgtcgaggg acgccgcggc gacgaacagg tcgccgaacg
     8701 gccggacctg accggcggcc tcggcgtcgg ggccgaccat gaagcccggc gtgtcgcaga
     8761 aggacacgac gggcagatcc cagcgctggc acagatgcac cagccgggtg gcgctggccg
     8821 ccccctcggc gtcgatggca cctccgaggt gggcggggtc gttggcgagc accccgaccg
     8881 cccgtccggc gatgcgcccg agtgcggtca ccatcccggg ggcgtgaccc gatcgcagct
     8941 ccacgaggga tcccgggtcg gtcaccgtcg ccaggatcga ccggacgtcg tagaggcgct
     9001 tgcgggcggc cgggacggcg tcgcgcagac ctgtctgatc ctctggtccg atcgccgtgt
     9061 ccacccgcgt cgacccgagt ccgaccagcg ctcgggcggc ccggaccgct gtgatgtcgt
     9121 cctcgacgcg gacgtcgacg gtcccgttgg cgacctggac ctcgatcggg ccgatcgcgg
     9181 tcggctcgac ccgtcccagg ccgccgccct cgatcatcgc gggtccggcc atccccaggt
     9241 tcgagtcctc ggtggcgacg atcacgtcgc agcacccgac cagggcggcg ttgccggcga
     9301 agcagtaacc cgacacgacc gccacggtcg ggacccgtcc gctgagccgg gcgaaccggg
     9361 cgaaggtcgg cacgtcgaga cccgtgacca ccggagcgtc aacgtctccg gggcgaccgc
     9421 cgccgccttc ggcgaacagc acgaccggga ggccgtgacg ttcgaccacc gcgaggatcc
     9481 gatcggtctt gcggtggttg acgaagccct gggtgccggc gaggacggtg gcgtcgtagc
     9541 cgatcaccgc gacggcgacg ccgtcgatcg tgccggtgcc ggtcagcagg ccgtcggccg
     9601 gggtccgttc gatcaggtcg tcgatctcgc gtcggccgcg ttgggcggcc accgcgaggc
     9661 cgccgtactc ggagaagctg tcggtgtcga ggagctgttc gaccagggag cggacggtgt
     9721 ggcggccctg gcggcgacgt cgtgcgaccg cctcggggcg catctcgtcg tgcaggaggg
     9781 agcggcggcg gagcacctcg gccaggtcgg ctcggtcgag gccgaccggt gcgtcctcct
     9841 cgacg
//
LOCUS       DAOQOD010000116         9817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2514_length_9817_cov_9.754251, whole genome shotgun sequence.
ACCESSION   DAOQOD010000116 DAOQOD010000000
VERSION     DAOQOD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_9817_cov_9.754251"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PLZ72_11265"
     CDS             <1..1169
                     /locus_tag="PLZ72_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA36919.1"
                     /translation="ESPPAGDAEGAGSDGERDDLLAERLRAVVDHLQWAVVIWDAEGQ
                     EIYRNRVARAMFEARDSQVLVAAAIEELRADALAGRSVRREVDVFGPPVGSFLVVARP
                     FRVRSLDDPGAAGSAGDAGAAQVDGAVAMVEDRTLQRRIETVRRDFVANISHELKTPI
                     GAVGLLAETVIDEPDPAVVERLATRMIAETDRLGRTVDDLLELSSIEFGDDAEFEDVE
                     LAALVAEAEDRLGSAPAQAGIEIRRDVPAGVVIHGDRRQLVSALANLVDNAVKYSADG
                     SEITVGATVAGAGEDDPEGTVLLSVADAGIGVPRRDLDRIFERFYRVDRARSRRTGGT
                     GLGLAIVRHVAANHGGDVRVESTEGVGSVFTLVLPPRHSAPPGPPDGSDGGDER"
     gene            1166..1933
                     /locus_tag="PLZ72_11270"
     CDS             1166..1933
                     /locus_tag="PLZ72_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA36920.1"
                     /translation="MSVAGAPGGPTNPTVLVVDDEEAFIEALAVGLGREGFDVEVARD
                     GAEALERFDEVAPDLVLLDVMLPTISGVDVCRALRQRSDVPIIMVTARASEIDTVVGL
                     EVGADDYVTKPYRLRELVARMRAVLRRHPRRDGHGVPAEPGGGPGPVDAFEDVLVVGD
                     VELDLDRHELRVRGEAVSVPLKEFELLTVLMENAGRVMPRSTLIDRVWGSDYVGDTKT
                     LDVHVKRLRAKVEPDPSHPTLITTIRGLGYKFAAPRP"
     gene            2010..3473
                     /locus_tag="PLZ72_11275"
     CDS             2010..3473
                     /locus_tag="PLZ72_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36921.1"
                     /translation="MSGHGEPVRLLEPRIDVRSPFGRLALTHVLSLAGDALVAMSLAG
                     TLFFDVDPSEARWKIALYLLFTMAPFAVVGPLIGPAMDRARGGHRAVMVGSTIGRAVI
                     AVLLIGPVGNDSLLLFPEAFLMLVMAKTYQVAKAAIVPTVVDGDAELVDANSKLQLLS
                     GFAGFAAGIPGGLLLLLLGPSAVVFLAAVVFAVGVIAALRVPSTTIAAEPAGDLEKAE
                     LRSAGVLSAASAMGTLRAVVGFVTFLLAFALRGGDKVAQLGTAVGRLAGSEHVGQDMS
                     PGDLVAPVGVPPAWHFGVIVAVSVAGGLAGAALAPTARRTIRNEESLLLAALGIGVAA
                     GLAGALLGGLAGQATMALLVAVAASSGKQAFDAVVQRDAPDANRGRSFARFESRFQVA
                     WVLGAFIPVVIPLPTRIGGWFVSLLSVAAAVFFALGMRAIGQGEAPPRLPTAREVGRG
                     VRSRVGSTRPDRRDPARRPPSAPMTSTADDDGDRPGQ"
     gene            complement(3435..4064)
                     /locus_tag="PLZ72_11280"
     CDS             complement(3435..4064)
                     /locus_tag="PLZ72_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36922.1"
                     /translation="MPAGPPLRFRPSPAVGRSRSPGRARARRRQVLAWAVVALVAVIV
                     ARTASAARADAARWQGDVAVLVLEESRSAGAPLGPDDVRVEHRPQAQVPAERTRSIPH
                     GATAAIRLPRGTVITDPLVRRDRRSATARALPAGTVAVALRTGDLPRLAEPGDSVDVA
                     APGWDGPVATGATVVRTDADSIVLAVDEGDAAVTAAASLAGPVAVVVRR"
     gene            complement(4160..5119)
                     /gene="map"
                     /locus_tag="PLZ72_11285"
     CDS             complement(4160..5119)
                     /gene="map"
                     /locus_tag="PLZ72_11285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNA36923.1"
                     /translation="MAKRKKRSRPLPPPPSPPVTAGLVSPLRTVPGSIARPDYAATGS
                     PSSRQSRAIRTDAEIEAMRVAGRVAAEALIEVGRHVAPGVTTDELDRIGHDAMVAAGS
                     YPSTLNYRGYPKSLCTSVNEIVCHGIPDSRRLRDGDIVNVDVTAFIEGVHGDTSATFL
                     VGDVDAASRALVDTTRRAMHAGISVVAPQARIRDIGRAIESVALPAGYSIVREFIGHG
                     IGDQFHTSLQIPHYYDPRNDTVLLAGMTFTVEPMVNVGAAGVTMWDDGWTVATADGQR
                     TAQFEHTLLVTEDGHELLTLPAEGPPAEVLFAPEPVGADTPTP"
     gene            5315..8296
                     /locus_tag="PLZ72_11290"
     CDS             5315..8296
                     /locus_tag="PLZ72_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36924.1"
                     /translation="MSRRSNAPAARGQMGIRSRLMLLLVVPLAGLLAVTGYAMVAAVQ
                     QSRDAAQLSGNADVALAAYGLVDALQAERHVLAAGSPTTDDMRAEVSDASERLRAEAA
                     EQGGLLQEMSQRALDRSAAASSLAASDTGGLVAVDGYSRAIASVLAVSRAGLDPHGAI
                     DDDPASAANLLASAQEAGAQEADLVQALAARGMLEPSSFGEVSELAAAQAIQSSQAAE
                     VADEDLTVRIDRVGDALAAASLGRDQAFFASARGAEIDPSALDDWLQGAEQRVSDLTS
                     LRDVAAARAVDAVDALGTSSRQVLLVSLGALIGVLLISAFLVRNAIRSIARPLSELAA
                     QAEDVALVRLPEAVRSQQTGDSDGRLPSLRATGATEVREVAGAFNDVQTTALRLAGEQ
                     AVLRRNLAEALTNLGRRNQALLGRQLDFISSLEQRETDPAFLEHLFKLDHLASRMRRN
                     AESLLILTGSETPRRRRKPAPLSEVVRAAMSEVEDFERVRLGQLGDATLTGPVVIDLV
                     HMLAELIENSLRFSPPDSTVEIDGRSLGQGGYQLAVIDHGVGMADVEIVAANERLAGL
                     DEVDGMPTRYLGQYVIAKLAAKTGALVRLQPTAGGRGITAVVSLPASATVGGTDRRSI
                     ARPLPGTLAARDQGSELIAPGAGLTPTVEQPDTVIDPTADPFADSVPSRDDLVADPMA
                     ASEEWSWTPDVVVLDDTAPLESAGAIDLEPREDLDAGAAAWVETGLAEDAHGWLDGAD
                     RPEPVDADGAVGSEIDPDHGSDSSDGSDGGPVATPEWLGSFDAVVLGDQDVTPEEIAA
                     AFPTGPPPPPPVIPEQDWAAEPAAVAEPTLPVEQTWPVEPTWPVEPAAADEVAPAEQT
                     GSDEPVPTAWSDPTPGAQPDEQPAEQPAEPVGGLAGGLTRRVPGASLQAGPLGQPAPE
                     AAPAVDRSADGVRSMLSAFQGGRSRGRGGVAVRDAELTEAADSGVASTETHPSPNPSP
                     MWAADLSGVEPTPELEDPRDH"
     gene            8286..8714
                     /locus_tag="PLZ72_11295"
     CDS             8286..8714
                     /locus_tag="PLZ72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNA36925.1"
                     /translation="MITDVNEQVQQIPWLLSRFVEQTPGVSDAVCVSSDGLLMAMSTD
                     LDRARADQFAAIAAGLTSLSLGASRCFGYGEVDQVMVEMSTGFLFVASISDGSCLGIL
                     STKDADVGLVGYEMTLLVERFGSVLTPQVRAGLRSVLAAG"
     gene            8747..9181
                     /locus_tag="PLZ72_11300"
     CDS             8747..9181
                     /locus_tag="PLZ72_11300"
                     /inference="COORDINATES: protein motif:HMM:NF017170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HNA36926.1"
                     /translation="MSAWDDPVTSGAPPASGVAPADPSVGSFAAEDVDDDEDAELVRP
                     FVITGGRTRHASVHLRVEALVVATGAAHDRQLQFEHARIIGQCNSPVSVAEVAARVGI
                     PLGVAQILVGDLAEAGLVQIHEARQAATPALLLRMIDAVRAL"
     gene            9162..9806
                     /locus_tag="PLZ72_11305"
     CDS             9162..9806
                     /locus_tag="PLZ72_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNA36927.1"
                     /translation="MQYEPSDPNARPPVGPGATPPPVGAAPQPVKILVAGGFGVGKTT
                     LVGAISEIEPLTTEATMTAYSEDVDDTSKIDRKTSTTVAMDFGRITIDQELILYLFGT
                     PGQERFWFMWDDLVDGALGAVVLLDTRRIQDCFAAVDFFESRGVPFVAVVNQFDDAGS
                     YSLEEVREALALPEHVPLVRADARARESVKMVLIALLEHLMQRAMSDHRATQRV"
BASE COUNT         1244 a         3611 c         3636 g         1326 t
ORIGIN      
        1 ccgagtcgcc gcccgccggc gacgccgagg gcgcgggctc cgacggagag cgggacgacc
       61 tgctcgccga acgcctccgg gcggtggtcg accacctgca gtgggcggtg gtcatctggg
      121 acgcagaggg ccaggagatc taccgcaacc gggtggcccg ggccatgttc gaggcccgcg
      181 actcccaggt gctggtcgcc gccgcgatcg aggagctgcg cgccgacgcg ctggccggac
      241 ggtcggtgcg gcgcgaggtc gacgtgttcg gcccgccggt cggctcgttc ctggtcgtcg
      301 cccgcccgtt ccgggtgcgg tcgctcgacg acccgggcgc tgccggttcc gctggcgatg
      361 ccggcgcggc ccaggtcgac ggggcggtgg cgatggtcga ggaccgcacc ctgcagcgcc
      421 gcatcgagac ggtgcgccgc gacttcgtcg ccaacatctc ccacgagctc aagaccccca
      481 tcggcgccgt cggactgctg gccgagacgg tgatcgacga acccgacccc gccgtcgtcg
      541 aacgcctggc cacccggatg atcgccgaga ccgaccggtt gggccgcacc gtcgacgacc
      601 tgctcgagct cagcagcatc gagttcggcg acgacgccga gttcgaggac gtcgagctgg
      661 ccgcgctggt ggccgaggcc gaggaccggc tcggctcggc accggcccag gccggaatcg
      721 agatccggcg tgacgtcccc gccggcgtgg tcatccacgg cgatcggcgc cagctcgtgt
      781 ccgcgctcgc caacctggtc gacaacgcgg tgaagtactc ggcggacggt tcggagatca
      841 ccgtcggcgc gaccgtcgcc ggagcaggcg aggacgaccc ggagggcacc gtgctcctca
      901 gcgtcgccga cgccggcatc ggcgtccccc ggcgggatct cgaccgcatc ttcgagcgct
      961 tctaccgggt ggaccgggcg cgctcgcgcc gcaccggcgg caccggcctg ggcctggcca
     1021 tcgtccgtca cgtcgccgcc aaccacggcg gcgacgtgcg cgtcgagtcc accgagggcg
     1081 tcgggtcggt cttcaccctc gtgctgccgc cccggcactc cgcaccgccc gggccccccg
     1141 atggctccga cggcggagac gagcgatgag cgtggcgggc gcgccgggcg gccccaccaa
     1201 cccgaccgtc ctcgtggtcg acgacgaaga ggcgttcatc gaggcgttgg cggtcgggct
     1261 gggccgcgag ggcttcgacg tcgaggtggc ccgcgacggt gccgaggcgc tcgaacggtt
     1321 cgacgaggtc gcccccgacc tggtcctgct cgacgtgatg ctcccgacga tctcgggcgt
     1381 cgacgtgtgc cgggcgttgc gccagcgcag cgacgtgccg atcatcatgg tcaccgcccg
     1441 ggcctctgag atcgacaccg tcgtcggtct cgaggtgggc gcggacgact acgtgaccaa
     1501 gccctaccgg ctgcgcgagc tcgtggcccg gatgcgggcg gtgctgcgcc gccatccgcg
     1561 ccgcgacggg cacggcgtgc cggcggagcc gggcggcggc cccggaccgg tcgacgcgtt
     1621 cgaggacgtc ctggtggtcg gagacgtcga actggacctc gaccgccacg agctgcgggt
     1681 gcggggcgaa gcggtgagcg tgccgctcaa ggagttcgag ctgctcacgg tgctgatgga
     1741 gaacgccgga agggtgatgc cgcgctcgac gctgatcgac cgggtgtggg gcagcgacta
     1801 cgtcggcgac accaagaccc tcgacgtcca cgtcaagcgg ctgcgggcca aggtcgagcc
     1861 cgacccgtcg cacccgacgc tgatcaccac catccgggga ctgggctaca agttcgccgc
     1921 gccccgaccc tgaccgtcgg ccgcaccctc gccgccgggc ggatcgggac cgaatcccgc
     1981 cgtcggagac ggctcgtagc atggcggccg tgagcggaca cggggagccc gtgcggctgc
     2041 tggagccgcg gatcgacgtc cggtcgccgt tcggtcgcct ggcgctcacc cacgtcctgt
     2101 cgctggccgg cgacgccctg gtggcgatgt cgctggccgg gacgctgttc ttcgacgtcg
     2161 acccgtccga ggcccgatgg aagatcgcgc tctacttgct gttcaccatg gcgccgttcg
     2221 ccgtggtcgg cccgctcatc ggcccggcca tggaccgggc gcggggcgga caccgggcgg
     2281 tgatggtcgg ctcgacgatc ggccgggcgg tgatcgcggt gctgctgatc ggaccggtcg
     2341 gcaacgacag cctgctgctg ttccccgagg cgttcctgat gctggtgatg gccaagacct
     2401 accaggtggc caaggccgcc atcgtcccca ccgtcgtcga cggcgacgcc gagctggtcg
     2461 acgccaactc caagctgcag ctgctgtcgg gcttcgccgg gttcgcggcc gggatccccg
     2521 gcggtctgct gctcctgctc ctcggcccga gcgcggtggt gttcctggcc gcggtcgtgt
     2581 tcgccgtcgg ggtgatcgcg gcgcttcggg tcccgtcgac gaccatcgcc gccgagccgg
     2641 ccggggacct cgagaaggcc gagctgcgca gtgcgggagt gctcagcgcg gcctcggcga
     2701 tggggacgtt gcgagcggtg gtcgggttcg tcaccttcct gctcgccttc gccctgcggg
     2761 gcggcgacaa ggtcgcacag ctcggcaccg cggtcggtcg gctcgccggc tccgagcacg
     2821 tcggccagga catgtcgccg ggggacctcg tcgcgccggt cggcgtgccg ccggcctggc
     2881 acttcggggt gatcgtcgcg gtcagcgtcg ccggcgggtt ggcgggagcg gcgctggcgc
     2941 ccacggcgcg acggacgatc cgcaacgagg agtccctgct gctcgccgcg ctcggcatcg
     3001 gcgtggcggc cgggctggcc ggagccctcc tcggaggact ggccggacag gcgacgatgg
     3061 cgctgctggt cgcggtggcc gcctcgtcgg gcaagcaggc cttcgacgcc gtggtgcagc
     3121 gcgacgcgcc cgacgccaac cggggtcgat cgttcgcccg gttcgagtcc cggttccagg
     3181 tggcgtgggt gctcggggcg ttcatcccgg tggtgatccc gctgcccacc cggatcggcg
     3241 ggtggttcgt gtccctcctg tcggtggccg ccgcggtctt cttcgccctc ggcatgcggg
     3301 cgatcggcca gggtgaagcc ccgccgcgcc tgccgacggc ccgcgaggtc gggcggggag
     3361 tgcggagtcg ggtgggctcc acccggccgg accgtcggga cccggcccgg cgcccgccgt
     3421 cggctccgat gacctcaacg gcggacgacg acggcgaccg gcccggccag tgacgccgcc
     3481 gcggtcacgg cggcatcgcc ctcgtccacg gccagcacga tcgagtccgc gtcggtccgg
     3541 acgaccgtcg cccccgtggc caccggtccg tcccagcccg gcgccgcgac gtcgacgctg
     3601 tcgccgggct cggccaggcg gggcaggtcg ccggtgcgca acgccaccgc caccgtcccc
     3661 gccggaaggg cccgcgcggt ggcgctgcga cggtcgcgac gcaccagcgg gtcggtgatc
     3721 accgtccccc gtgggagccg aatcgccgcg gtcgcgccgt gggggatcga ccgggtccgc
     3781 tcggcgggca cctgggcctg gggtcggtgc tcgacgcgga cgtcgtcggg gccgaggggt
     3841 gccccggccg accgcgactc ctcgagcacc agcaccgcga cgtcgccctg ccagcgggcc
     3901 gcgtcggccc gggcggcgct cgcggtgcgc gccacgatga ccgcgaccag tgcgaccacc
     3961 gcccaggcca ggacctgacg tcgtcgcgcc cgggcgcgcc cgggggagcg tgaccgcccc
     4021 acggcgggtg acgggcggaa tcggagtggg ggacctgcag gcacgggggc tccctcgggg
     4081 cgcggtccgg ctcggaccgt cggggtcggg ggaagcgacg aggggaccgc ccagccggaa
     4141 gtgtcggggg cgggtcccct cagggggtcg gcgtgtcggc cccgaccggc tcgggtgcga
     4201 agagcacctc ggccggcggc ccctcggcgg gaagggtcag cagctcgtgg ccgtcctcgg
     4261 tcaccagcag cgtgtgctcg aactgggcgg tgcgctggcc gtccgcggtg gcgacggtcc
     4321 agccgtcgtc ccacatggtc accccggcgg cgccgacgtt gaccatcggc tccaccgtga
     4381 aggtcatgcc cgccagcagc accgtgtcgt tgcgcgggtc gtagtagtgc gggatctgca
     4441 gcgacgtgtg gaactggtcg ccgatgccgt gaccgatgaa ctcgcgcacg atcgagtagc
     4501 cggccggcag cgcgaccgac tcgatggccc ggccgatgtc gcggatccgg gcctgtgggg
     4561 cgacgaccga gatgcccgcg tgcatggccc ggcgggtggt gtcgaccagg gcccgggagg
     4621 ccgcgtcgac gtcgccgacc aggaacgtgg ccgaggtgtc gccgtgcacg ccctcgatga
     4681 aggcggtgac gtcgacgttg acgatgtcgc cgtcgcgcag acggcgcgag tcggggatcc
     4741 cgtggcagac gatctcgttg accgacgtgc acagcgactt ggggtagccg cggtagttga
     4801 gggtcgacgg gtacgaaccc gccgcgacca tggcgtcgtg gccgatgcgg tcgagctcgt
     4861 cggtggtcac cccgggagcc acgtggcgtc cgacctcgat cagcgcctcg gcggccaccc
     4921 gaccggccac ccgcatggcc tcgatctcgg cgtcggtgcg gatggcgcgg gactgccgcg
     4981 acgacggcga ccccgtggcc gcgtagtccg gtcgggcgat gctgccgggc acggtgcgca
     5041 acggtgacac caacccggcc gtcaccggcg gtgacggagg aggcggaagg ggacgtgatc
     5101 gcttcttgcg cttggccacc gcgccagcgt acgtggccgc aggacgaccg accggccgtc
     5161 ggatccgggg cgcgcgaccc gggggtggac gacgtcgggg gtccgaccgt cggcgggatc
     5221 ggccggtgcc ggtggcgccc ggatcccgga tggtccgttc ggacgtgggc cgaaattctc
     5281 aacccgatgg gccggacggt cgacacggat cacgatgtca cgccgatcga acgctccagc
     5341 ggcccgcggg cagatgggca tccgcagccg cctcatgctc ctcctggtcg tcccgctcgc
     5401 gggactcctc gccgtcaccg gctacgccat ggtggccgcc gtccagcagt cccgcgacgc
     5461 cgcgcagctc agcggcaacg cggacgtggc cctggccgcg tacgggctgg tcgacgccct
     5521 gcaggccgag cgccacgtgc tcgccgcggg ctcgccgacc accgacgaca tgcgcgccga
     5581 ggtgtccgac gccagcgaga ggctgcgggc cgaggccgcc gagcagggcg ggctgctcca
     5641 ggagatgagc cagcgggcgc tcgaccgctc cgcggccgcc tcgtcgctgg ccgccagcga
     5701 caccggaggt ctggtcgcgg tcgacggcta ctcgcgtgcc atcgcctcgg tcctggcggt
     5761 gagccgcgcc gggctcgacc cgcacggcgc catcgacgac gaccctgcgt cagcggccaa
     5821 cctcctcgcc tccgcgcagg aggccggagc ccaggaggcc gacctggtcc aggcgctggc
     5881 cgcgcggggc atgctggagc cgtcgtcgtt cggcgaggtg tcggagctgg ccgctgcgca
     5941 ggcgatccag tcgtcccagg ccgccgaggt ggccgacgag gacctgaccg tccgcatcga
     6001 ccgggtgggc gacgccctgg ctgcggcgtc cctcgggcgg gaccaggcct tcttcgcctc
     6061 ggcccggggt gccgagatcg acccctccgc cctcgacgac tggctgcagg gggccgagca
     6121 gcgggtgtcg gacctgacgt cgctgcgtga cgtcgccgcg gcccgggccg tcgacgccgt
     6181 cgacgccctc ggaacctcga gccgtcaggt gctgctggtg tccctcggcg ccctgatcgg
     6241 cgtgctgctc atcagcgcct tcctggtccg caacgccatc cggtcgatcg cccgtccgct
     6301 gtccgagctc gccgctcagg ccgaggacgt cgccctggtc cggctgcccg aagcggtgag
     6361 gagccagcag accggcgaca gcgacggacg gctgccatcg ctgcgggcga ccggagccac
     6421 cgaggtccgc gaggtggccg gcgcgttcaa cgacgtgcag accaccgctc tgcgcctggc
     6481 cggcgagcag gccgtcctgc gccgcaacct ggccgaggcc ctgacgaacc tcggtcgacg
     6541 caaccaggcc ctcctcggtc gccagctcga cttcatctcc tccttggagc agcgggagac
     6601 cgatcccgcc ttcctcgagc acctcttcaa gctggaccac ctggccagcc gcatgcgacg
     6661 caacgcagag tcgctgctga tcctgaccgg gtccgagacc cctcgccgac gtcgcaagcc
     6721 cgccccgctg agcgaggtcg tgcgcgcggc gatgagcgag gtggaggact tcgagcgggt
     6781 ccggctcggc cagctcggcg acgccaccct gaccggcccg gtcgtgatcg acctcgtgca
     6841 catgctggcc gagctgatcg agaactcgct gcggttctcg ccgccggact ccaccgtgga
     6901 gatcgacggg cgctccctgg gccagggcgg ctaccagctg gcggtgatcg accacggcgt
     6961 cggcatggcc gacgtcgaga tcgtcgccgc caacgagcgc cttgccggcc tggacgaggt
     7021 cgacgggatg ccgacccggt acctgggcca gtacgtgatc gccaagctcg ccgccaagac
     7081 cggggcgctg gtgcggttgc agccgaccgc cggcggccgg ggcatcaccg cggtggtgag
     7141 cctgcccgca tcggccaccg tcggaggcac ggaccgacgg tcgatcgcac ggccgctgcc
     7201 gggcacgctc gcggcgcggg accagggcag cgagctcatc gcccccggcg ccggcctcac
     7261 cccgaccgtc gagcagcccg acaccgtgat cgacccgact gcggacccct tcgccgacag
     7321 cgtccccagc cgtgacgacc tcgtcgccga tccgatggca gcgtcggagg agtggtcatg
     7381 gacacccgac gtggtggtcc tcgacgacac cgctccgctc gagagcgcgg gcgcgatcga
     7441 cctcgagccc cgtgaggacc tggacgccgg tgccgcagcg tgggtcgaga ccggtctcgc
     7501 agaggacgcc cacggctggc tggacggagc ggatcgtcct gagcccgtgg acgcggacgg
     7561 cgcggtgggc tccgagatcg accccgatca cggctcggac agctccgacg gttccgacgg
     7621 cgggccggtg gccacccccg agtggctcgg ctcgttcgat gccgtcgtcc tgggcgacca
     7681 ggacgtcacc cccgaggaga tcgccgcggc gttcccgacc ggaccgccgc ctccgcctcc
     7741 ggtgatcccg gagcaggact gggccgccga gccggccgcg gtcgctgagc cgacgctgcc
     7801 tgtcgagcag acctggcctg tcgagccgac ctggcctgtc gagccggccg ccgccgacga
     7861 ggtggccccg gcggagcaga ccgggagcga cgagcccgtc ccgaccgcgt ggagcgatcc
     7921 gacaccgggc gcccagccgg acgagcagcc ggccgagcag ccggcagagc cggtcggcgg
     7981 tctggccgga gggctgaccc gtcgggtgcc gggagcgtcg ctgcaggccg gcccgctcgg
     8041 ccagcccgca ccggaggccg caccggcggt ggaccgctcc gcggacggcg tccgctccat
     8101 gctgtccgcc ttccagggcg gccgctcccg gggtcgtggc ggcgtggccg tccgcgacgc
     8161 cgagctcacc gaggccgccg actccggcgt cgcctccacc gagacccacc cgtccccgaa
     8221 cccgtccccc atgtgggcgg ccgatctctc gggcgtcgag ccgacgccgg aactggagga
     8281 tccccgtgat cactgacgtc aacgagcagg tgcagcagat cccctggctg ctgtcgcgct
     8341 tcgtcgagca gacccccggt gtcagcgacg ccgtgtgcgt cagctccgac ggactgctga
     8401 tggcgatgtc gaccgacctc gaccgggccc gtgccgatca gttcgcggcc atcgccgccg
     8461 gtctgaccag cctgtcgctc ggtgcctcgc ggtgcttcgg ctacggcgag gtcgatcagg
     8521 tgatggtcga gatgagcacc gggttcctgt tcgtcgcctc gatctccgac ggcagctgcc
     8581 tgggcatcct gtcgaccaag gacgccgatg tcggcctggt cggctacgag atgaccctgc
     8641 tggtcgaacg gttcggctcg gtgctgaccc cgcaggtccg tgccggtctg cgcagcgtcc
     8701 tggccgccgg ctgagcccga ccccgcaccg aggagcagtc cgacccgtgt ccgcctggga
     8761 cgatcccgtc acctccggcg ctccgccggc gagcggcgtc gcgcccgcgg acccgtcggt
     8821 cggctcgttc gccgccgagg acgtcgacga cgacgaggac gcggagctgg tccgcccgtt
     8881 cgtcatcacc ggcggccgca cccgtcacgc cagcgttcac ctgcgggtgg aggcgctggt
     8941 ggtcgccacc ggggccgcgc acgaccgaca gctccagttc gagcacgccc ggatcatcgg
     9001 gcagtgcaac tcgccggtct ccgtcgccga ggtggcggcg cgggtcggca tccccctggg
     9061 cgtcgcccag atcctggtgg gcgacctggc cgaggccgga ctcgtccaga tccacgaagc
     9121 ccgtcaggcc gccacgcccg cactcctgct gaggatgatc gatgcagtac gagccctctg
     9181 acccgaacgc ccgccccccg gtcggtcccg gcgccacgcc accccccgtg ggtgcggcgc
     9241 cccagccggt caagatcctg gtcgccggag gcttcggggt gggcaagacc acgctggtgg
     9301 gggcgatctc cgagatcgag ccgctgacca ccgaggcgac catgacggcg tactccgagg
     9361 acgtcgacga cacctccaag atcgaccgca agacgtcgac caccgtcgcc atggacttcg
     9421 gtcgcatcac catcgaccag gagctgattc tctacctgtt cgggaccccc ggccaggagc
     9481 gcttctggtt catgtgggac gacctggtcg acggggcgct cggcgccgtg gtgctgctcg
     9541 acacgcgccg catccaggac tgcttcgcgg cggtggactt cttcgagagc cgaggcgtcc
     9601 cgttcgtcgc ggtcgtcaac cagttcgacg acgccggctc gtactccctc gaggaggtgc
     9661 gcgaggccct cgccctgccc gagcacgtgc cgctggtgcg agccgatgcg cgggcccggg
     9721 agtcggtgaa gatggtgctc atcgccctgc tcgagcacct gatgcagcgg gccatgtcgg
     9781 accaccgcgc cacccagcgg gtctgagccc ccgccca
//
LOCUS       DAOQOD010000117         9765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2533_length_9765_cov_13.846241, whole genome shotgun sequence.
ACCESSION   DAOQOD010000117 DAOQOD010000000
VERSION     DAOQOD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_9765_cov_13.846241"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..229
                     /locus_tag="PLZ72_11310"
     CDS             62..229
                     /locus_tag="PLZ72_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36928.1"
                     /translation="MPRSIQIRDVDDDVYEALAKRAAEVGLSVPEYLRRKIARLAAAT
                     GPRCNIDVPSA"
     gene            complement(288..716)
                     /gene="mce"
                     /locus_tag="PLZ72_11315"
     CDS             complement(288..716)
                     /gene="mce"
                     /locus_tag="PLZ72_11315"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNA36929.1"
                     /translation="MSENPDATSGLLTEIDHVAIAVRDLDSAVDYYQRAFGATVAHRE
                     VVDSDGVEEALVKVADSYIQLTAATRPDSPIAKAIEKRGEGLHHVGYRVANCQEALDA
                     MVAAGATPIDKAPRPGSRGTTVAFIHPKGSFGTLIELVQE"
     gene            complement(760..2109)
                     /gene="ccrA"
                     /locus_tag="PLZ72_11320"
     CDS             complement(760..2109)
                     /gene="ccrA"
                     /locus_tag="PLZ72_11320"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNA36930.1"
                     /translation="MQEILEAIQTGASGDDIANLTLPESTRAAHVLRSEAGMWEGVDS
                     WDKDPRQSLHVGELPLPELAPDEAVVAVMASSINFNTVWTSIFEPLPTFGFLDRLGKE
                     SVWGARHAQDFHIVGSDASGVVVRVGSAVRNWKPGDRVTVHCNYVDDQDPSAHNDSML
                     AANQRIWGFETNYGGLADLSIVKANQLMPKPAHLSWEESAVNALCASTSYRMLVGDHA
                     GRMKQGDNVFIWGATGGIGAYATQLVINGGGTPIGVVSSESRVKLLNAMGCDAVIDRR
                     AEGYQFWSDEHTQDESEWRRLGKKVRSLVGEDPDIVFEHPGRSTMGASVFITKRGGKI
                     VTCAATSGYMLEYDNRHLWMKLKSIISSHFANYQEAWEMNRLIDKGAIQPVMSEVYAL
                     DQVGDAALKVHHNEGEGKIGVLCLAPEAGQGITDAAKREAIGEDRITLYQRHARGEL"
     gene            complement(2186..3388)
                     /locus_tag="PLZ72_11325"
     CDS             complement(2186..3388)
                     /locus_tag="PLZ72_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA36931.1"
                     /translation="MIATAAHAEVTTAFVQLGLILLGLGLAARVADRAGFSPIPLYLL
                     GGLAVRAAGLLPAKVTDDFIGIAAEIGVLQLLFALGLEYTAPELSAELRRNFRAGIVD
                     LVANFLPGLLLGLVLGWSAVASVLLGGVSYVSSSGIIAKLLGDLGRLGNRETPTILSI
                     LVLEDLTMAIYLPIVAVLLAGAGVLTGLVSLAVALGVVAVIVLFAVKIGPWLSRVVDS
                     ESNEVTVLVILGLVLFVGGLVQEVDVSAAVGAFLVGLALSGPVQHRAATLIEPLGNVF
                     AAIFFFVFGLGIDATAVPPVILPALGLAVVTAATKAGLGWWAAGRAGVGKHGRWRAAT
                     TLIVRGEFSIVIAGLGVAAGVEGQLGPLAAAYVLVLAVVGSLLVRVARLPGFLDRSAR
                     GESRPGTV"
     gene            complement(3388..3870)
                     /locus_tag="PLZ72_11330"
     CDS             complement(3388..3870)
                     /locus_tag="PLZ72_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNA36932.1"
                     /translation="MTEIHETELPGVGVLQEFDTVGGSRVGVLTTRAGRRELLFYDDE
                     DPDSCRAMVHLDPEDGLVLADLLGGTHVVDAAASLQRLEGLAIDWLTVHRSSALAGST
                     IGDAGVRQGTGASIVAIIRGSSTNAAPGPADRLEVGDVVVAVGTPEAVERLTALVESG
                     "
     gene            3950..4552
                     /locus_tag="PLZ72_11335"
     CDS             3950..4552
                     /locus_tag="PLZ72_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA36933.1"
                     /translation="MVAGTPRPLLELRTERLLLRPWRDSDREPFAAINADPVVMEHFP
                     STLSRDESDDFVDRVIAGWAARGFGLWAVEVTEPEDDGPAGFVGHVGLMSAEHRGAGT
                     VEVGWRLAREAWGRGYAPEAARAALGVGFDSLGLDEVVSFTVPQNTNSRRVMDKIGMT
                     HRPERDFDHPSVDPLTDPHLVRHVLYSITADEWAALATGR"
     gene            4609..5790
                     /locus_tag="PLZ72_11340"
     CDS             4609..5790
                     /locus_tag="PLZ72_11340"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNA36934.1"
                     /translation="MPGSVILAGARTPIGKLSGSLAGFSGTDLGGLAIKAALERAGVA
                     GSDVDYVFMGQVLLAGAGQMTARQAAVAGGIPMDVPSTTVNKVCLSGIHSIMLADLLI
                     QAGLADVVVAGGMESMTNAPYLLPDARAGMRMGDKKVIDSMMYDGLFCAFDQCAMGAG
                     TEKYAASAGIARDEQDQLAATSHERAAKAQKDGLFDDEIVPVSIPQRKGDPVEFSADE
                     GVRAETTLDSLGALRPAFDKAGNITAGNASQISDGGAAVVVASAAAAERIGGDPLGRI
                     VSFGEVAGPDASLLTQPSRSILQALERAGKTVGDVDLFELNEAFAAVGVASMKDLGVS
                     DDIVNVNGGAIALGHPIGMSGTRITLHILNELKRRGGGLGAAALCGGGGQGDALLVET
                     I"
     gene            5948..7750
                     /locus_tag="PLZ72_11345"
     CDS             5948..7750
                     /locus_tag="PLZ72_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA36935.1"
                     /translation="MDHPRRSEDRPGADDQVSQTERAQQAVRRFRSELTLGEDSIDTD
                     TWAGSIPQVYGVAPRVRVGRSRWFNLLWLVPIAAVGLVVAIPAAQGLRNVPAVADFIE
                     RYPGADPAWSAGAGTGFPAWLSWQHFFNLFLMVFIVRAGLQILADHPRLYWTRHSTPG
                     HEWFRMQKPVPEDPLWTAKEDSIRLPSGVGLPGVRHTIGLARWWHLGTDVVFLLNGVV
                     FYVLLFVTGQWHRVVPTSWDVLPNAASTMIQYLSLDWPPEKGWVVYNGLQLIAYFVTV
                     FVAAPLALVSGLGMSPALSTRFRRISKVLSIQFARSLHFLVMVWFLVFIAMHVTMVFT
                     TGLLNNLNHMFAGRNDDSWVGFWVFAVAMVVVGIAWWAASPFTLRHPRVVQRVGSTLI
                     GPFERWFAHVDAQPGEYDESDISPYFWHNGRYPDSAEYRTLADSGFVDYRLEISGRVE
                     HPVELSLEDLRALPPHEQITQHFCIQGWSGVAKWGGVSMTTILDLVEPHPDAEWVVFY
                     SFGEGADGGIYYDAHPIEEMRGELAMLAFDMNGEPLPFGYGAPLRLRNEVQLGFKQVK
                     WIKGIELVAGIDEVGGGYGGYNEDHEFFGYRQAI"
     gene            complement(7846..8490)
                     /gene="recR"
                     /locus_tag="PLZ72_11350"
     CDS             complement(7846..8490)
                     /gene="recR"
                     /locus_tag="PLZ72_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNA36936.1"
                     /translation="MGTAYADSVQDLIDALGRLPGIGPKSAQRIAFHLLKVDAEEANR
                     LARAIVEAKEKVGFCARCFNLAEGDLCAICADDRRDGHVLCVVEEPRDVVAVERTREF
                     RGRYHVLQGAIDHLQGVGPEQLRIRELLARVGSEGITEVIVCTNPNIEGEATALYLTR
                     VLDTPGLTVSRIASGLPVGGDLEYADELTLGRALEGRRVVERPDAPAERDLSPG"
     gene            complement(8491..8967)
                     /locus_tag="PLZ72_11355"
     CDS             complement(8491..8967)
                     /locus_tag="PLZ72_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNA36937.1"
                     /translation="MNTPPDNDGESAPTDEVLSGEVVATPDGADSVDGSPEQPGDLPG
                     ALGGLDMGAMLQMAQDMGERMQEAQEALATTEIVGSAGGGLVTVTLNGHLHVRAVHIE
                     PGAVDADDPTVLEDLIVAAWTDAHNGVARLQAQADPLGGVGGLGGALGGLGGLLGG"
     gene            complement(8986..9582)
                     /locus_tag="PLZ72_11360"
     CDS             complement(8986..9582)
                     /locus_tag="PLZ72_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36938.1"
                     /translation="MAVTAGSAPLDRDGLTVVFGDRVVPQLKGVAKAIYSSGHFVAVT
                     GRGAVFTLPNAATVERAEKFRPDVESMIGAEVGSPVRLLLVDETDPAAVADATGGPGR
                     SAASSTSASAPTPTPTQTPTPPRGRTTPRTRSEEPAVAVPEPTTAPDPVEPTGDDDES
                     SIIDVHDLEDADVAATGVEKLTQAFPGAVLVDGADGAS"
BASE COUNT         1424 a         3446 c         3454 g         1441 t
ORIGIN      
        1 cggaactgcg gccagtcccc aacacctcgg gacatgcaat atcgcatgag acaatgcgtc
       61 catgccccgc tcgatccaga tccgggacgt tgacgatgac gtctatgagg cgctcgccaa
      121 gcgggcagcc gaggtcggcc tatcggtccc cgagtacctg cgccggaaga ttgctcggct
      181 cgccgcggcg accgggcctc ggtgcaacat cgacgtgccg tccgcgtgaa ctcaacggag
      241 ccggctcggc ggggccagct ccgcggccgc cggcccagcg gggccggcta ctcctggacc
      301 agctcgatca gcgtgccgaa gctgcccttg gggtggatga aggcgacggt ggtgccccgc
      361 gacccggggc ggggggcctt gtcgatcggc gtggcgccgg ccgcgaccat ggcgtcgagc
      421 gcctcctggc agttcgccac ccggtagccg acgtggtgca gaccctcgcc ccgcttctcg
      481 atcgccttgg caatcggtga gtcgggacgc gtggctgcgg tcagctggat gtagctgtcg
      541 gccaccttga ccagcgcctc ctcgacgccg tcggaatcga cgacctcacg atgggcgacg
      601 gtggcaccga aggcccgctg gtagtagtcg accgcggagt cgaggtcgcg caccgcgatg
      661 gcgacgtggt cgatctcggt cagcagaccg gatgtggcgt cggggttctc gctcatgtcg
      721 tctctcctcg ttctcagtgc gcggtgccgg agcggccggt cacagctcgc ctcgggcatg
      781 gcgctggtag agggtgatgc ggtcctcgcc gatcgcctcg cgcttggcgg cgtcggtgat
      841 gccctggccg gcctcgggcg ccaggcacag cacgccgatc ttgccctcgc cttcgttgtg
      901 gtggaccttc agcgcggcgt cgccgacctg gtcgagcgcg tagacctcgc tcatcaccgg
      961 ctggatcgcg cccttgtcga tcagccggtt catctcccac gcctcctggt agttggcgaa
     1021 gtgggagctg atgatgctct tgagcttcat ccacaggtgg cggttgtcgt actcgagcat
     1081 gtagcccgag gtggcggcgc aggtgacgat cttgccgccg cgcttggtga tgaacaccga
     1141 cgcgcccatg gtcgaacgac ccgggtgctc gaagacgatg tcgggatcct cgccgacgag
     1201 cgaacggacc ttcttgccca ggcgacgcca ctcggactcg tcctgggtgt gctcgtcgga
     1261 ccagaactgg tagccctcgg cccgacggtc gatcaccgcg tcgcagccca tggcgttgag
     1321 cagcttgacc cgcgactccg aggacacgac gccgatcggg gtgccgccgc cgttgatgac
     1381 cagctgggtg gcgtaggcgc cgatgccgcc ggttgcgccc cagatgaaca cgttgtcgcc
     1441 ctgcttcata cgcccggcgt ggtcgccgac gagcatccgg tagctggtcg aggcgcacag
     1501 cgcgttgacc gctgactcct cccagctgag gtgcgccggc ttgggcatca gctggttggc
     1561 cttgacgatc gacaggtcgg cgagtccgcc gtagttggtc tcgaagcccc agatgcgctg
     1621 gttggccgcc agcatcgagt cgttgtgcgc ggaggggtcc tggtcatcga cgtagttgca
     1681 gtggacggtg acccggtcac ccggcttcca gttgcgcacc gcggacccga cccgcaccac
     1741 gacgccggac gcgtcggatc cgacgatgtg gaagtcctgg gcgtggcgcg ccccccacac
     1801 cgactccttg cccagccggt cgaggaaccc gaaggtcggc agcggctcga agatcgaggt
     1861 ccacaccgtg ttgaagttga tcgagctggc catcaccgcg acgacggcct cgtcgggggc
     1921 gagctcgggc aggggcaact cgccgacgtg caggctctgg cgcgggtcct tgtcccagga
     1981 gtcgacgccc tcccacatgc cggcctccga gcgcagcacg tgggcggccc gggtggactc
     2041 cgggagcgtg aggttcgcga tgtcgtcgcc ggacgcgccg gtctggatgg cctcgaggat
     2101 ctcctgcatg ggcgctgaag ctaccggtcg gtaaccgcac cggccagcac gagcaccgtg
     2161 tggcgccccc atcggtggtg gggcttcaga ccgtgcccgg cctgctctca ccgcgagcag
     2221 agcggtcgag gaacccgggc aggcgagcga cacggaccag cagggagccg acgacggcca
     2281 gcaccagcac gtaggcggcg gccagcgggc cgagctgacc ctccaccccc gcggcgaccc
     2341 cgagtccggc gatgacgatc gagaactcgc cgcggacgat cagagtggtc gcggcgcgcc
     2401 agcggccgtg cttgcccact ccggcccgac cggcggccca ccaccccagt ccggccttgg
     2461 tcgcggcggt cacgacggcc agaccgagcg ccgggaggat caccggcggg acggcggtgg
     2521 cgtcgatgcc caggccgaac acgaagaaga agatggcggc gaacacgttg cccaacggct
     2581 cgatgagggt cgcggctcgg tgctgcaccg gaccggacaa cgcgaggccg accaggaacg
     2641 caccgacggc cgcggacacg tcgacctcct gaacgagtcc gccgacgaac aggacaagac
     2701 ccaggatgac cagcacggtc acctcgttcg actcggagtc gaccacccgt gacaaccagg
     2761 gtccgatctt gacggcgaac aggacgatca ccgcgaccac gccgagggcg acggcgagcg
     2821 acaccagacc ggtcagcacc cccgccccgg ccaacagcac ggccacgatc ggcaggtaga
     2881 tcgccatcgt caggtcctcg agcaccagga tcgacaggat cgtcggggtt tcgcggttgc
     2941 cgaggcggcc gaggtcgccc agcagcttgg cgatgatccc cgacgacgac acgtaggaga
     3001 caccaccgag cagcaccgac gcgaccgcgg accagccgag cacgagtccg agcagcagac
     3061 cgggcaggaa gttggcgacc aggtcgacga tcccggcccg gaagttgcga cgcagctcgg
     3121 ctgacagctc cggcgccgtg tactcgagtc ccagagcgaa caggagctgc agcacgccga
     3181 tctcggccgc gatcccgatg aagtcgtcgg tgaccttggc cgggagcaac ccggccgccc
     3241 ggaccgccag accgccgagc aggtagagcg ggatcggact gaagccggcg cggtccgcga
     3301 cccgggccgc cagacccagg cccagcagga tgagccccag ctggacgaag gccgtggtga
     3361 cctcggcgtg ggccgcggtc gcgatcatca gcccgactcg accagcgcgg tcagtcgctc
     3421 gaccgcctcg ggggtcccga ccgccacgac cacgtcgccc acctcgaggc ggtccgcggg
     3481 accgggggcg gcgttggtcg acgatccccg gatgatggcg acgatgctgg ccccggtgcc
     3541 ctgccgcact cccgcgtcgc cgatggtcga tccggccagg gccgaggacc ggtgcacggt
     3601 caaccagtcg atggccagcc cctcgaggcg ttgcagcgag gccgcggcat cgaccacgtg
     3661 cgtgccgccg agcagatcgg cgagcacgag gccgtcttcg gggtcgaggt gcaccatggc
     3721 gcggcacgag tcggggtcct cgtcgtcgta gaacagcagc tcacggcggc cggcacgggt
     3781 ggtcagcacc ccgacccggg atccgcccac ggtgtcgaac tcctggagca cccccacgcc
     3841 ggggagctcg gtctcgtgga tctcggtcac ggcacctccc tgcgggaccg atcggagcgg
     3901 tccaccccgt tcgagcggcc cagcctagga cgcccgtacg atcaccccgg tggtcgccgg
     3961 cacaccccgt cccctcctcg agctgcgcac cgagcgactg ctgctgcgcc cgtggcggga
     4021 ctccgaccgg gaaccgttcg cggcgatcaa cgccgatccg gtggtcatgg agcacttccc
     4081 ctcgaccctg agccgcgacg agtccgacga cttcgtcgac cgagtgatcg cgggttgggc
     4141 cgcgcggggc ttcggcctct gggcggtcga agtgaccgaa cccgaggacg acggcccggc
     4201 cgggttcgtc ggccacgtcg gcctcatgtc tgccgagcac cgcggcgcgg ggaccgtcga
     4261 ggtcgggtgg cggctggccc gagaggcctg ggggcggggc tacgccccgg aagcggcccg
     4321 cgccgcgctc ggcgtgggct tcgactcgct cggtctggac gaggtcgtca gcttcaccgt
     4381 gccgcagaac accaacagcc gacgggtgat ggacaagatc ggcatgaccc accggccgga
     4441 gcgcgacttc gaccacccct cggtcgatcc gctcaccgat ccgcatctgg tgcgacacgt
     4501 cctgtactcg atcaccgccg acgagtgggc cgccctcgct accgggcggt aacatcgccg
     4561 aaccgcagga gggggccgtg cggctccccc gacctctctg gagtggacat gcctggttcg
     4621 gtgattctcg cgggggcccg gacccccatc ggcaagctgt ctggttcgct ggccgggttc
     4681 tccggcacgg acctcggcgg cctcgccatc aaggccgccc tcgagcgcgc cggggtggcg
     4741 ggatccgacg tcgactacgt cttcatgggc caggtgctgc tcgccggtgc cggccagatg
     4801 accgcccgtc aggccgcggt cgccggtggg atccccatgg acgtcccgtc gaccacggtc
     4861 aacaaggtgt gcctgtcggg catccactcg atcatgctgg ccgacctcct gatccaggcc
     4921 ggactcgccg acgtggtcgt cgccggtggc atggagtcca tgaccaacgc cccctacctg
     4981 ctgcccgacg cccgggccgg catgcgcatg ggcgacaaga aggtcatcga ctcgatgatg
     5041 tacgacggcc tgttctgcgc cttcgaccag tgcgcgatgg gcgccggcac cgagaagtac
     5101 gcggcgtccg ccggcatcgc ccgcgacgag caggaccagc tggcggccac gtcgcacgag
     5161 cgggcggcca aggcccagaa ggacggcctg ttcgacgacg agatcgtccc ggtgtcgatc
     5221 ccgcagcgca agggcgaccc cgtcgagttc agcgccgacg agggcgtccg cgccgagacc
     5281 accctcgact cgctcggcgc cctgcgaccc gcgttcgaca aggccggcaa catcaccgcc
     5341 ggcaacgcgt cgcagatctc cgacggcggc gccgcggtcg ttgtcgcctc ggccgccgcg
     5401 gccgagcgca tcggcggtga ccccctcggg cgaatcgtga gcttcggcga ggtcgccgga
     5461 ccggacgcgt cgctgctcac ccagccgtcc cgatcgatcc tgcaggccct ggagcgggcg
     5521 ggcaagaccg tcggcgacgt ggacctgttc gagctcaacg aggcgttcgc cgcggtgggt
     5581 gtcgcgtcga tgaaggacct gggcgtctcc gacgacatcg tcaacgtgaa cggcggggcg
     5641 atcgcgctgg gccacccgat cggcatgtcg ggcacccgga tcaccctgca catcctcaac
     5701 gagctcaagc gccggggcgg cggcctcgga gcggccgcac tgtgcggcgg cggcggccag
     5761 ggcgacgccc tgctcgtcga gacgatctga ccgctgcgat ccgcggcagg ccgccgcgga
     5821 tcgcacacag ctgacgggga tgggccccgg ccgattcgtc ggtcggggcc catccgcgtc
     5881 cggggtcgac ccgggcccgg acgggccaag tactggatcc accagcaggt gggtagacag
     5941 gtgatcgatg gatcaccccc gccgctccga ggaccgaccc ggagctgacg accaggtgag
     6001 ccagacggag cgggcacagc aggcggtccg caggttccgc tcagagctca ccctcggcga
     6061 ggactcgatc gacaccgaca cgtgggcggg ttcgatcccc caggtgtacg gggtggcacc
     6121 gagggtccgc gtcggccggt cccgctggtt caacctgctg tggctggtcc ccatcgccgc
     6181 ggtcggactg gtggtcgcga tcccggccgc ccagggcctg cgcaacgtgc ccgccgtggc
     6241 cgacttcatc gagcgctacc ccggcgcgga cccggcctgg agcgcaggcg caggaaccgg
     6301 gttcccggcg tggctgagct ggcagcactt cttcaacctg ttcctgatgg tcttcatcgt
     6361 gcgcgccggc ctgcagatcc tcgccgacca ccctcgcctc tactggaccc gccacagcac
     6421 gcccggacac gagtggttcc ggatgcagaa gccggtgccc gaggacccgc tgtggacggc
     6481 gaaggaggac tccatccgtc tgccgtcggg cgtcggcctg ccgggggtgc gccacacgat
     6541 cggattggcc cgctggtggc acctggggac cgacgtcgtc ttcctgctca acggcgtggt
     6601 gttctacgtg ctgctgttcg tcaccgggca gtggcatcga gtcgttccga ccagctggga
     6661 cgtgctcccc aacgcggcgt cgacgatgat ccagtacctg tcgctcgact ggccgcccga
     6721 gaagggctgg gtcgtctaca acggcctcca gctcatcgcc tacttcgtga ccgtgttcgt
     6781 cgcggccccg ttggcgctgg tgagcgggct cgggatgtca ccggcgctgt cgacgcggtt
     6841 ccggcgcatc agcaaggtgc tctccatcca gttcgcccgg tccctgcact tcctcgtgat
     6901 ggtctggttc ctggtcttca tcgccatgca cgtgacgatg gtgttcacca ccggcctgct
     6961 caacaacctc aaccacatgt tcgccggccg caacgacgac agctgggtcg ggttctgggt
     7021 gttcgccgtg gccatggtcg tggtcggcat cgcctggtgg gccgcgtcac cgttcacgct
     7081 gcgccacccc agggtggtcc aacgcgtggg gtccaccctg atcggcccgt tcgaacgctg
     7141 gttcgctcac gtcgacgccc aacccggcga gtacgacgag tccgacatct cgccgtactt
     7201 ctggcacaac ggtcgctacc ccgactccgc tgagtaccgg accctggccg acagcgggtt
     7261 cgtcgactac cggctggaga tcagcgggcg ggtcgaacat ccggtcgagc tcagtctgga
     7321 ggacctgcgc gccctgcctc cccacgagca gatcacccag cacttctgca tccaggggtg
     7381 gtccggcgtg gccaagtggg gaggggtgtc gatgacgacg atcctcgacc tggtcgagcc
     7441 gcaccccgac gccgagtggg tcgtcttcta ctcgttcggc gagggtgccg acggcggcat
     7501 ctactacgac gcccatccga tcgaggagat gcgcggcgag ctggccatgc tggccttcga
     7561 catgaacggc gaaccgctgc cgttcggcta cggcgccccg ctgcggctgc gcaacgaggt
     7621 gcagctcggg ttcaagcagg tcaagtggat caaggggatc gagctggtcg ccggcatcga
     7681 cgaggtcggc ggcggctacg gcggctacaa cgaggaccac gagttcttcg gataccgcca
     7741 ggcgatctga cagtccggcg atctgacagt ccggcgaggc cgccgggcga accggacgcc
     7801 gcgcgatccg aacggcgggc gatccgaatg gcgccgtccg cggcgtcagc ccggcgacag
     7861 gtcccgctct gcgggtgcgt cggggcgttc gacgacccgg cgaccctcga gcgcccgccc
     7921 gagcgtgagc tcgtccgcgt actccaggtc gcctccgacc ggcagaccgc tggcgatgcg
     7981 gctcacggtc agccccggcg tgtcgagcac ccgggtcagg tacagcgccg tggcctcgcc
     8041 ctcgatgttg gggttggtgc agacgatgac ctcggtgatc ccctcggaac cgacgcgggc
     8101 gaggagctcc ctgatgcgca gctgttcggg gccgaccccc tggaggtggt cgatggcgcc
     8161 ctgcagcacg tggtagcggc cccggaactc gcgggtgcgt tcgacggcga cgacgtcccg
     8221 gggctcctcg acgacgcaca gcacgtgtcc gtctcgtcgg tcgtcggcgc agatcgcgca
     8281 caggtccccc tcggccaggt tgaagcaacg ggcgcagaac ccgaccttct ccttggcctc
     8341 gacgatcgcc cgggccaggc ggttggcctc ctcggcgtcg accttgagca ggtggaacgc
     8401 gatccgctgg gcggacttgg ggccgatgcc ggggagtcga ccgagggcgt cgatgaggtc
     8461 ctggacggag tcggcgtacg cggtgcccac tcagcccccc agcaggccgc cgaggccccc
     8521 gagcgctcct ccgaggccgc cgacgcctcc gagtggatcg gcctgcgcct ggagtcgcgc
     8581 cacgccgttg tgcgcgtcgg tccacgccgc gacgatcagg tcctcgagca cggtcgggtc
     8641 gtcggcgtcg accgccccgg gctcgatgtg aaccgcgcgg acgtgcaggt gaccgttgag
     8701 cgtgacggtg accagtccgc cgccggcgga gccgacgatc tcggtggtcg ccagcgcctc
     8761 ctgcgcctcc tgcatgcgtt cgcccatgtc ctgtgccatc tggagcatcg cgcccatgtc
     8821 gagaccgccg agcgctccgg gcagatcacc gggttgctcc ggggaaccgt cgacgctgtc
     8881 ggcgccatcg ggtgtggcga cgacctcgcc gctcagcacc tcgtcggtgg gggccgactc
     8941 tccgtcgttg tccgggggcg tgttcatcag ggttcctcgg gtcggtcagg acgcaccgtc
     9001 ggcgccgtcc acgaggaccg cgcccgggaa cgcctgggtg agcttctcca cgccggtggc
     9061 ggccacatcg gcgtcctcca ggtcgtgcac gtcgatgatc gacgactcgt cgtcgtcgcc
     9121 ggtcggttcg accgggtcgg gggccgtggt cggctcgggc accgcgacgg caggctcctc
     9181 ggagcgcgtc cggggtgtcg tcctgccacg cggcggcgtc ggagtctgag tcggcgtcgg
     9241 cgtcggggcc gacgccgagg tgctggacgc ggcggaccga ccgggcccgc ccgtcgcatc
     9301 ggcgaccgcc gccgggtcgg tctcgtcgac cagcagcagc cggaccggtg aacccacctc
     9361 ggcgccgatc atcgactcga cgtccggtcg gaacttctcg gcccgctcca cggtggccgc
     9421 gttggggagc gtgaagacag ccccacgtcc ggtcaccgcg acgaagtggc ccgaggagta
     9481 gatcgccttg gccacaccct tgagctgggg caccacccgg tcgccgaaga cgacggtcaa
     9541 cccgtcgcgg tccagcggcg cggatccggc cgtgacggcc acgctgtccg atgggggtgc
     9601 cggcggcagg gatcggtccg cagcggtcgt ctccgggttt gccgacgggg tggggtcctt
     9661 ctcggcggcc ggtcggggcg gcggagacga cgggacagcc ggtgccgccg gtgccgccgg
     9721 tgcctctggc gccgcggcgg gtcgggcaga cggtcggggt gcgac
//
LOCUS       DAOQOD010000118         9709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2559_length_9709_cov_9.942200, whole genome shotgun sequence.
ACCESSION   DAOQOD010000118 DAOQOD010000000
VERSION     DAOQOD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9709
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2559_length_9709_cov_9.942200"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLZ72_11365"
     CDS             complement(<1..240)
                     /locus_tag="PLZ72_11365"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA36939.1"
                     /translation="MEHIVSGVGVLDKSCALLAVVAQGPCTLADLSERTAVSRATTHR
                     LVVALEQHGMLRRTGDGRVALGLHLVGLGRAAAQQF"
     gene            320..1735
                     /gene="leuC"
                     /locus_tag="PLZ72_11370"
     CDS             320..1735
                     /gene="leuC"
                     /locus_tag="PLZ72_11370"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNA36940.1"
                     /translation="MSAEERPRTLAEKVWDRHVVRSAEGEPDLLYIDLHLVHEVTSPQ
                     AFDGLRMAGRTVRRPDLTVATEDHNVPTADIDAPIADPISRKQVETLRANAEEFGIVE
                     FPMGSPQQGIVHIIGPEQGRTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLAT
                     QTLPQARPKTLAVTVDGDLRPGVTAKDVILAILGVLGTSGGIGHIAEYRGSAIRSLSM
                     EGRMTVCNMSIEAGAKAGLIAPDEVTFEYLKGRPHAPQGDEWDRAVADWRTLVTDEGA
                     TFDKEVFLDAADIEPFVSWGTNPGQVIPLSASIPSPDDFDDQVLRDAATRALEYMDLS
                     AGTPMRQVPVDTIFIGSCTNGRIEDLRAAAEVARGRRVADGVRTLVVPGSHAVKAQAE
                     AEGLDEVFRTAGFDWREPGCSMCLAMNPDKLSPGERCASTSNRNFEGRQGRGGRTHLV
                     SPAVAAATAIAGHFAAPADLA"
     gene            1756..2346
                     /gene="leuD"
                     /locus_tag="PLZ72_11375"
     CDS             1756..2346
                     /gene="leuD"
                     /locus_tag="PLZ72_11375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNA36941.1"
                     /translation="MDAVRIITGTAVPLDRSDVDTDQIIPSDWLKQVERTGFDKGLFS
                     EWRDDRDFVLNQEQFQGANILIAGPNFGTGSSREHAVWAIQQYGFAAVVSPRFGDIFR
                     NNSTKNGLVPVQVSAEVGRQLLDAVAADPTLQFVIDIERRTLECDSLGLVMDFPLDDS
                     VQHRFLEGLDDIGLSLRHDADITTFEAQRPAWMPTA"
     gene            2466..>3644
                     /locus_tag="PLZ72_11380"
     CDS             2466..>3644
                     /locus_tag="PLZ72_11380"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNA36942.1"
                     /translation="MGTTHRSEATDRRGTAPPTRGRSERSPLRSRSLGIRSALLVVAA
                     LALLAGCTQDIERVSGPRTGEPTQSDIRLVRAETCDQLVAAAEAREQIARDRMERLEG
                     DGQDAVAFDEGATGSGAAGGAERSPGASTPATTMAPSPVAAPAAGPTDADPAAASDQS
                     TTPKGDDTSGAPVIAGTNNQEQGVDEGDMVKTDGRLLVTLTGDGVLRVVVLDDSPTVD
                     GQLTLSGTGSAQPSHMYGMTTGQLLLRGDEVVAVQVAWDGRGMPITDITRVDLSDPST
                     PRVTERARVAGELAATRMIGRTIRIVVRPSLGTPIEPMPTPMPEPPMPPETTVPEPTT
                     TTTTTTTATSTSTTTTTGTDPAEIPAAAARLMPQLVGDDGTARPLGGCQDVLSAPMVE
                     P"
     assembly_gap    3645..3744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3745..4911
                     /locus_tag="PLZ72_11385"
     CDS             <3745..4911
                     /locus_tag="PLZ72_11385"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNA36943.1"
                     /translation="TVDGGVESVYAGTDALYTTSTVWGGPTGEMTAVHRFDLTGEGAA
                     RYTGSGLVPGMLLNQYSMSDRGGALRIVTTATTMVPAAPAATSTVLPSPRPTAIPVEP
                     GTAVEPDGSAVSPAEPGIAADVVGPMTTSAGRLAVLRPADDGMLREVGHLDDLGVGEQ
                     VKAVRFLGDRAYVVTFRQTDPLFAVDLTDDTAPRLLGELKIPGFSEYLHPIGDGRLLG
                     IGSDADATTGRVTGFKASLFDVTDPTAPKELDALVAPDMTSPVGQDPHTFTWDPTRSQ
                     AIVPVMSYGVADEGAVPPMGECPEGAACAMPAVRVDESAGVDQAARPWSGAWVLGVDG
                     DRIVRRGSIAHDLGPAAADILRSVVVGDSIWTVSDRAVGRTPAAAPDGVTLIRF"
     gene            complement(5074..6657)
                     /gene="serA"
                     /locus_tag="PLZ72_11390"
     CDS             complement(5074..6657)
                     /gene="serA"
                     /locus_tag="PLZ72_11390"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNA36944.1"
                     /translation="MRVLVSEKIADGGLQRLRDAGHEVDIQLELTPETLLDAVQGSHA
                     LIIRSATKVTREVLEAGADLVVVGRAGIGLDNVDTSAATERGVMVVNAPQSNTLSAAE
                     HTMALLLAQARNVPQAHAALKEGRWERSKWEGVELSDKTLGIIGLGRIGKLVAQRAMA
                     FGMKLVAYDPFVTPDRARQINVELVDLDTLMARADFVTLHLAKTPETAGLIGAELLAK
                     AKPSLRIINVARGGIIDEAALAAAIESGTIAGAALDVFAAEPTTESPLFGLDSVVVTP
                     HLGASTAEAQDKAGDTIAEMVELALAGDFVPFAVNVSAGEAAESVRPYLGLAERLGAI
                     LAGLCGSDLGGGPAGSADSIEIEYQGQIAEHDTRILTLSVLKGFFGATSGEPVSYVNA
                     PQVAEEQGIEVRPTATTTARDYVNLISLRSGQHSLAGTLVGLRAVPTVVMIDDHAVDV
                     PPSDHLLIVRNEDRPGMIGTVGTVLGAASVNIADMAVGANEDGAKALMAIVTGDSVPD
                     EVVAELRALDGITSVNAVG"
     gene            6889..8049
                     /locus_tag="PLZ72_11395"
     CDS             6889..8049
                     /locus_tag="PLZ72_11395"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNA36945.1"
                     /translation="MTVAAAGLLAACIPPTPEVPPSGPTAPTTAPTTAPTTTVPGPGG
                     DVTVAGTVSGFSRPWDIAFLPDGTPIVTERSGRVATVVAGARHVVATLADVEPIGEGG
                     LMGLAVDPAFGTNRWIYTCYDHGSAGTVTDVRIVRFRLAEDLRSLTDATPILTGIPAG
                     SGNRHQGCRVAFGPDGMLWATTGDTASPTAPQDPTSRAGKVLRMTPAGAPAPGNPGGA
                     WDPYVYSFGHRNGQGLTFRPADGATIEVEHGTGCDDEINRIVAGGNYGWNPVGSGYDE
                     SKPMTDLTIPGARPALWSSGCPTIAPSGATFVSGAEWGSWNGALAVAVLKGTRLMFVR
                     FDGPTADTVAGVDVRLSDRGRLRTVRQAPDGSLWVTQDSSPGALLRLVPTTA"
     gene            complement(8077..>9709)
                     /locus_tag="PLZ72_11400"
     CDS             complement(8077..>9709)
                     /locus_tag="PLZ72_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNA36946.1"
                     /translation="EPVNEIAAVALGGPSGSPEPVEPVTLVAEGDWAASPALSPDGTV
                     LAWLRWDHPDMPWDAAELWAGRLVYPESDVGSGANPGGAAHPVVADARRVAGGRAGAA
                     ARSVGHPVAACLPAWSPDGHLWWCDDAEDWWHLRRSGSSGLPDEGEGDTGPPVLEVPG
                     EVGEPRWVSGGSRFGFTDDGRVVVALTHGGVDTVQVWDPGLGTVDALASPPGGFTAVE
                     HLVVLGSVVAVAGGGGTVPTSAYSVDLRTDRVTDHRAADPLSRPHLSAASISTPTAVT
                     FPTADGDHAHALFYPPCSTNSVGPAGELPPLVVRIHGGPTAAARAELSPSVQFWTSRG
                     VAVVEVNYRGSTGYGRHYRDLLRGRWGVADVADCIAAARWLSEQGLVDGARCVIRGGS
                     AGGFTALEATCRSEVFAAACSLYGVTDLRALATDTHKFESRYLDGLVGPYPQEAERYR
                     ERSPLFHAELLDSPVLLLQGTEDPIVPPSQAEVLRDALRSGGIPHALILFDGEAHGFR
                     SAATIVAALQAELAFYGQVLGFEPADDLPPLTLDR"
BASE COUNT         1411 a         3655 c         3342 g         1201 t
ORIGIN      
        1 gaactgctga gcggccgccc ggccgagccc gaccaggtgc agcccgagtg cgacccggcc
       61 gtcgccggtg cgacgcagca tcccgtgctg ctcgagcgcg acgaccaggc ggtgcgtcgt
      121 cgcccgcgac accgcggtcc gctccgacag gtccgccagc gtgcacggcc cctgcgccac
      181 cacggcgagc agcgcgcacg acttgtcgag caccccgacc ccgcttacga tgtgttccat
      241 cagtcgggaa ctgtatctca ctatctgaga tcccccaacc cgacgacaga gactccactc
      301 cccgaaaggc gacgccgaga tgagcgcaga ggaacggccc cgaacgctgg ccgagaaggt
      361 gtgggaccgc cacgtggtgc ggtccgccga gggcgaaccc gacctgctct acatcgacct
      421 ccacctggtc cacgaggtca cctcgccgca ggcgttcgat ggccttcgca tggccggccg
      481 caccgtgcgc cgccccgacc tgaccgtcgc caccgaggac cacaacgtcc ccaccgccga
      541 catcgacgcc ccgatcgcgg acccgatctc gcgcaagcag gtcgagacgc tgcgggccaa
      601 cgccgaggag ttcggcatcg tcgagttccc gatgggatcg ccccagcagg gcatcgtgca
      661 catcatcggc cccgagcagg gccgcaccct gcccggcatg accatcgtct gcggcgacag
      721 ccacacctcg acccacgggg cgttcggtgc gctggcgttc ggcatcggca cctccgaggt
      781 cgaacacgtc ctggccaccc agaccctccc gcaggcccgc ccgaagaccc tggcggtcac
      841 cgtcgacggc gacctgcgcc cgggggtcac cgccaaggac gtgatcctgg ccatcctcgg
      901 cgtcctcggc acctcgggcg gcatcggcca catcgccgag taccgcggct cggccatccg
      961 ctccctgtcg atggaggggc gcatgaccgt gtgcaacatg tcgatcgagg ccggcgccaa
     1021 ggccggcctc atcgcccccg acgaggtcac cttcgagtac ctgaagggac gcccgcacgc
     1081 cccgcagggc gacgagtggg accgagcggt ggccgactgg cgcacgctgg tcaccgacga
     1141 gggcgccacc ttcgacaagg aggtcttcct cgacgcggcg gacatcgagc cgttcgtatc
     1201 ctggggaacc aaccccggtc aggtcatccc cctgtcggcg tccatcccgt caccggacga
     1261 cttcgacgac caggtcctgc gcgacgcagc gacccgggcg ctcgagtaca tggacctgag
     1321 cgccggcacc ccgatgcgcc aggtgccggt cgacacgatc ttcatcggca gctgcaccaa
     1381 cggccgcatc gaggacctcc gcgccgcggc cgaggtggcc cgcggccgcc gcgtcgccga
     1441 cggcgtgcgc accctggtcg tccccggctc gcacgcggtc aaggcccagg cggaggccga
     1501 gggcctcgac gaggtgttcc gcaccgcagg gttcgactgg cgcgagccgg gctgctccat
     1561 gtgcctggcc atgaaccccg acaagctctc ccccggcgag cgctgcgcct ccacctcgaa
     1621 ccgcaacttc gagggacgcc agggccgcgg cggacgcacc cacctggtgt cgcccgccgt
     1681 cgccgcggcc accgccatcg ccgggcactt cgccgccccc gccgacctcg cctgaccgag
     1741 aggatcacca caccaatgga cgccgtccgc atcatcaccg gcaccgcggt ccccctcgac
     1801 cgctccgacg tcgacaccga ccagatcatc cccagcgact ggctcaagca ggtcgagcgc
     1861 accggcttcg acaagggcct cttctcggag tggcgcgacg accgggactt cgtgctgaac
     1921 caggagcagt tccagggcgc caacatcctg atcgccggac cgaacttcgg caccggctcg
     1981 agccgcgaac acgcggtgtg ggccatccag cagtacgggt tcgccgcggt ggtctcgcca
     2041 cggttcggcg acatcttccg caacaactcg accaagaacg gcctggttcc ggtgcaggtg
     2101 tctgccgagg tcggccgcca gttgctcgac gcggtcgcag cggacccgac cctgcagttc
     2161 gtgatcgaca tcgagcggcg gaccctggag tgcgactcgc tgggcctggt catggacttc
     2221 ccgctcgacg actcggtgca gcaccgcttc ctcgagggcc tcgacgacat cggcctgagc
     2281 ctgcgccacg atgccgacat caccacgttc gaggcccagc gaccggcctg gatgccgacc
     2341 gcctgagcgg gcgatcggtg ccaggtacgg agaatctctc cggatccggg aacctgaccg
     2401 cacctcccga cgtctgaccc ctcgaacgca cgccggacgt gccggggacg acacgaggag
     2461 gaccgatggg caccacccac cgcagcgagg cgaccgaccg gcgaggaacg gcgccgccga
     2521 cccgagggcg atcggagcga tcgccgctcc ggtcgcgatc gctcggcatc cggtcggcgc
     2581 tgctcgtcgt ggccgcactg gcgctgctgg ccggatgcac ccaggacatc gaacgggtgt
     2641 cagggccccg caccggcgag cccacccaga gcgacatccg cctggtgcgc gccgagacct
     2701 gcgaccagct ggtcgccgcg gctgaggctc gcgagcagat cgcccgcgac cggatggagc
     2761 gcctcgaggg ggacggccag gacgccgtcg ccttcgacga gggagcgacc gggtccgggg
     2821 ccgccggagg ggcggagcgc tcccccggcg catcgacgcc ggccaccacg atggccccct
     2881 cccccgtcgc tgccccggcg gccggaccga cggacgcaga ccccgcagcc gcgtccgacc
     2941 agagcacgac gccgaagggc gacgacacga gcggcgcacc ggtgatcgcc ggcaccaaca
     3001 accaggagca gggcgtcgac gagggcgaca tggtcaagac cgacggacgg ctcctcgtca
     3061 ccctcaccgg tgacggggtg ctccgcgtgg tcgtgctcga cgactccccc accgtcgacg
     3121 ggcagctgac cctcagcggg acgggctccg cgcagcccag ccacatgtac gggatgacca
     3181 ccggacagct gctgctgcgc ggcgacgagg tggtcgccgt gcaggtcgcg tgggacgggc
     3241 ggggcatgcc gatcaccgac atcacccggg tggacctgag cgacccgagc acgcctcggg
     3301 tcacggagcg ggcccgggtg gccggcgagc tggccgccac ccggatgatc ggtcggacga
     3361 tccgcatcgt cgtccgaccg tcgctcggca caccgatcga accgatgccc acgccgatgc
     3421 ccgagccgcc gatgccgccg gagacgacgg ttcccgaacc gacgacgacg accacgacga
     3481 ccacgacggc tacgtcgacc tcgacgacca cgacgaccgg caccgatccg gccgagatcc
     3541 cggccgccgc cgcccggttg atgccccagc tcgtcggcga cgacggcacc gcccgcccgc
     3601 tcggcgggtg ccaggacgtc ctgtcggccc cgatggtgga acccnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnaccgtc gacggcgggg tcgagtcggt ctacgccggc
     3781 accgacgccc tgtacaccac gtcgacggtg tggggcggac cgaccggcga gatgaccgcc
     3841 gtgcaccgct tcgacctgac cggtgagggc gccgcccgct acaccggcag cggactggtg
     3901 ccggggatgc tgctcaacca gtactcgatg tcggaccgag gcggcgccct gcgcatcgtg
     3961 accaccgcca cgaccatggt gccggctgct ccggcggcca cgtcgaccgt cctgccgtcg
     4021 ccccggccga cggccatccc ggtagagccg gggaccgcgg tcgagcccga cgggtcggcc
     4081 gtctcccccg ccgaaccggg tatcgccgcc gacgtggtcg gcccgatgac cacctcggcg
     4141 ggccggctgg cggtcctgcg gcccgccgat gacggcatgc tgcgcgaggt cggccacctc
     4201 gacgacctcg gcgtgggcga acaggtgaag gcggtccggt tcctcggcga ccgcgcctac
     4261 gtggtcacgt tccgccagac cgacccgctg ttcgccgtcg acctgaccga cgacaccgca
     4321 ccgcggctgc tgggcgagct caagatcccc ggcttctctg agtacctgca cccgatcggc
     4381 gacggccgcc tcctgggcat cggctccgac gcggacgcca ccaccggccg ggtgaccggg
     4441 ttcaaggcgt cgctgttcga cgtgaccgac ccgaccgcac ccaaggagct cgacgccctc
     4501 gtcgcgcccg acatgacctc accggtcggc caggacccgc acaccttcac ctgggacccg
     4561 acccgctcgc aggcgatcgt gccggtgatg agctacggcg tcgccgacga gggtgcggtc
     4621 cccccgatgg gcgagtgccc cgagggcgcg gcgtgcgcca tgccggcggt gagagtcgac
     4681 gagtcggcgg gggtcgacca ggcggcgcgg ccgtggagcg gggcgtgggt cctcggcgtc
     4741 gacggcgacc ggatcgtccg gcggggctcc atcgcccacg atctcgggcc cgcggcggcg
     4801 gacatcctgc gctcggtggt ggtcggcgac tcgatctgga cggtgtcgga ccgtgccgtg
     4861 ggtcgcaccc ccgccgccgc tcccgatggc gtcaccctga tccggttctg aatccaaccg
     4921 atcacggccg ggcgctgccg tcgaacttgt agctgaccgg tcgccacagc gaccgaggcc
     4981 tacaagaacg ccggcagccg tcgaacttgt agctgaccgg tcgccacagc gaccgggggc
     5041 tacaagttcg gtgggggttc gcgccggcag cgctcagccg acggcgttga ccgacgtgat
     5101 gccgtccagc gcgcggagct cggcgacgac ctcgtcgggc accgagtcgc cggtgacgat
     5161 cgccatcagg gccttggccc cgtcctcgtt cgccccgacg gccatgtcgg cgatgttgac
     5221 cgacgcggcg ccgagcaccg tgccgaccgt cccgatcatt cccggccggt cctcgttgcg
     5281 gacgatcagc aggtggtccg acggcggcac gtcgaccgcg tggtcgtcga tcatcaccac
     5341 ggtcggtacc gcccgcagcc cgaccagggt gccggccagg ctgtgctggc cgctgcgcag
     5401 cgagatcagg ttcacgtagt cacgggccgt ggtggtggcc gtcgggcgca cctcgatccc
     5461 ctgctcctcg gcgacctgcg gggcgttcac gtacgacacc ggctcgccgc tggtcgctcc
     5521 gaagaacccc ttgagcaccg acagggtgag gatgcgggtg tcgtgctcgg cgatctggcc
     5581 ctggtactcg atctcgatcg agtccgcgct gccggcgggt ccgccgccga ggtcgctgcc
     5641 gcacaggcca gccaggatcg ccccgaggcg ctcggcgagg ccgagatacg gccgcaccga
     5701 ctcggcggcc tcacccgcgc tcacgttgac cgcgaacggg acgaagtcgc cggcgagggc
     5761 cagctcgacc atctcggcga tcgtgtcgcc ggccttgtcc tgggcctcgg cggtcgaggc
     5821 gcccaggtgc ggggtgacca cgacggagtc caatccgaac agcggcgact cggtggtcgg
     5881 ctcggcggcg aacacgtcga gggcggctcc ggcgatcgtg ccggactcga tggcggcggc
     5941 cagcgccgcc tcgtcgatga tgccgccgcg ggccacgttg atgatccgca ggctcggctt
     6001 ggccttggcc agcagctcgg cgccgatcag accggcggtc tcgggcgtct tggccaggtg
     6061 cagcgtcacg aagtcggccc gggccatcag cgtgtcgagg tcgaccagct cgacgttgat
     6121 ctgccgggcc cggtcggggg tgacgaacgg gtcgtaggcg acgagcttca tgccgaacgc
     6181 catcgcccgt tgcgccacca gcttgccgat gcggcccagt ccgatgatgc cgagggtctt
     6241 gtcggacagc tcgacgccct cccacttgga gcgctcccag cgaccctcct tgagcgcggc
     6301 gtgtgcctgg ggcacgttgc gggcctgggc caggagcagc gccatcgtgt gctcggccgc
     6361 cgacagggtg ttggactgag gggcgttgac caccatcacg ccgcgttcgg tggccgccga
     6421 ggtgtcgacg ttgtcgaggc cgatcccggc ccggcccacg acgaccaggt cggcgccggc
     6481 ctcgaggacc tcgcgggtga ccttggtcgc ggagcggatg atgagcgcgt gggagccctg
     6541 gacggcgtcc agcagggtct cgggggtcag ctccagttgg atgtcgacct cgtggccggc
     6601 atctcgcagc cgctgcaggc cgccgtccgc gatcttctcg ctcaccagta ctcgcatggc
     6661 gtccatcctt gcccgtccgc gccgacgcgg ccagatggcg gcggaccacg cgctccagtt
     6721 ccgatgcccg atgtgccgat ggggggctgc agctcccgac cgcgccgacc cggagaacga
     6781 actgtgccca ccgcgaccaa cccccgcacg accgacgacc aaacccgacc gggctccgcc
     6841 cgggcacgga cgcgacgccg agccgcacgg ccgacgctcc tcgccgccgt gacggtcgcc
     6901 gcggccgggc tgctggccgc gtgcatccct ccaaccccgg aggtcccgcc gtcgggcccg
     6961 accgcaccga cgacggcacc cacgacagca ccgacgacga cggttcccgg cccgggtggc
     7021 gacgtcaccg tcgccgggac ggtcagcggg ttctcccgac cgtgggacat cgccttcctg
     7081 cccgacggca ccccgatcgt caccgagcga tccggtcggg tcgccaccgt cgtcgcgggg
     7141 gcacgccacg tcgtggccac cctggccgac gtggaaccga tcggcgaggg cggcctgatg
     7201 ggcctggccg tcgaccccgc gttcgggacc aaccgctgga tctacacctg ctacgaccac
     7261 ggatcggccg gcaccgtcac cgacgtgcgc atcgtgcggt tccgcctggc cgaggacctg
     7321 cgctcgctga ccgacgccac cccgatcctg accgggatac cggcgggctc gggcaaccgc
     7381 caccagggct gccgggtcgc cttcggcccc gacggaatgc tgtgggccac caccggcgac
     7441 accgcctcgc cgaccgcgcc gcaggacccg accagccggg ccggcaaggt gctgcgcatg
     7501 acacccgccg gtgctcccgc ccccggcaac cccggcgggg cctgggaccc gtacgtgtac
     7561 tcgttcggac accgcaacgg ccagggactg acgttccgac ccgccgacgg cgccacgatc
     7621 gaggtggagc acggcaccgg ctgcgacgac gagatcaacc ggatcgtcgc cggcggcaac
     7681 tacggctgga acccggtggg atcgggctac gacgaatcca agccgatgac cgacctcacc
     7741 atccccggtg cccgacccgc gctgtggtcg agcgggtgcc cgacgatcgc gccatccggc
     7801 gccacgttcg tgtcgggcgc ggaatgggga tcgtggaacg gggcgctcgc cgtcgcggtg
     7861 ctgaaaggca cccggctgat gttcgtgcgc ttcgacggtc ccaccgccga cacggtggcc
     7921 ggcgtcgatg tccgactcag cgaccggggc cggctgcgca ccgtccgcca ggcccccgac
     7981 ggcagcctgt gggtcaccca ggactcgagc ccgggtgccc tgctgcgcct ggtcccgacc
     8041 accgcctgag acccggtcgg tgcgggcgag gaccgctcag cgatcgaggg tgagcggcgg
     8101 caggtcgtcg gcgggctcga agccgagcac ctggccgtag aaggcgagct cggcctggag
     8161 cgcggcgacg atggtggccg cgctgcggaa cccgtgggcc tcgccatcga acaggatgag
     8221 ggcgtggggg atgccgccgc tgcgcagggc gtcgcgcagc acctcggcct gcgacggcgg
     8281 gacgatcggg tcctcggtgc cctgcagcag cagcaccggc gagtcgagca gctcggcgtg
     8341 gaacagcggc gatcgctccc ggtagcgctc ggcctcctgc gggtacggcc cgaccaggcc
     8401 gtcgaggtag cgggactcga acttgtgggt gtcggtggcg agcgcccgca ggtcggtgac
     8461 cccgtacagc gagcaggccg ccgcgaagac ctctgatcgg cacgtcgcct cgagtgcggt
     8521 gaacccgccc gcggagccgc cccggatcac gcaccgggcg ccgtcgacca ggccctgctc
     8581 ggacaaccag cgtgccgcgg cgatgcagtc ggcgacatcg gccacccccc agcgaccccg
     8641 cagcaggtct cggtagtggc gtccgtaccc ggtcgagccg cggtagttga cctcgaccac
     8701 cgccacgccc cgcgacgtcc agaactgcac cgacggcgac agctcggctc tggccgccgc
     8761 ggtcgggccg ccgtggatgc gcacgaccaa cggggggagc tcgccggccg ggcccaccga
     8821 gttggtcgag cacggcgggt agaacagggc gtgggcgtga tcgccgtcgg cggtcgggaa
     8881 cgtgaccgcg gtcggcgtcg agatcgacgc cgcggaaagg tgcggtcgtg acaacgggtc
     8941 ggcggcccgg tggtcggtca cccggtccgt ccggagatcg accgagtacg ccgaggtggg
     9001 gacggtccct ccccctccgg ccaccgcaac caccgacccc aggaccacga ggtgctcgac
     9061 cgcggtgaac ccgcccggcg gcgatgccag agcgtcgacc gtcccgaggc cggggtccca
     9121 gacctgcacg gtgtcgaccc ccccgtgggt gagcgcgacg acgacgcggc cgtcgtcggt
     9181 gaagccgaac cgtgacccgc ccgagaccca ccggggttcg cccacctcgc cggggacctc
     9241 cagcaccggc gggcccgtgt cgccctcacc ctcgtcgggc agtccggacg acccgctgcg
     9301 gcgcaggtgc caccagtcct cggcgtcgtc gcaccaccac aggtggccgt cgggcgacca
     9361 ggccggcagg caggccgcga ccggatgccc cacgctgcgg gcggcagcac ccgcccggcc
     9421 gccggccacc cgccgggcgt cggccacgac cgggtgcgcg gcaccgccgg gattcgctcc
     9481 ggagcccacg tcggactcgg ggtagacgag ccggcccgcc cacagctccg ccgcgtccca
     9541 cggcatgtcg ggatggtccc agcgcagcca cgccagcacc gtcccgtccg gcgacaacgc
     9601 cggggacgcg gcccagtccc cctccgcgac gagcgtcacc ggctcgaccg gctcgggcga
     9661 tcccgagggg ccaccgagag cgaccgccgc gatctcgttg accggctcg
//
LOCUS       DAOQOD010000119         9707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2561_length_9707_cov_10.229072, whole genome shotgun sequence.
ACCESSION   DAOQOD010000119 DAOQOD010000000
VERSION     DAOQOD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_9707_cov_10.229072"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="cobA"
                     /locus_tag="PLZ72_11405"
     CDS             complement(<1..882)
                     /gene="cobA"
                     /locus_tag="PLZ72_11405"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNA36947.1"
                     /translation="MTVHLVGAGPGDPGLITVRGAELLARADVVVHDRLSARELLDLA
                     PPDAELIDVGKSPDGPSTPQSAINELLVERGRRGQTVVRLKGGDPFVFARGAEEAAAL
                     AAAGVDYEVVPGITSALAVPAYAGIPVTLRYSSTSVTIVTGHEDPSKGRTDVGWEALA
                     RVGGTIVVLMGVRNLPRIAERLVAGGLSPDTPAAAIRWGTRPTQQTVRATLSTLADHD
                     LASPATIVIGDVAGERLDWFERRPLLGRTVVVTRTRDQSPQLARRLRDAGAEVLVAPT
                     IAVEDPATEPVDRGCGLR"
     gene            complement(879..1838)
                     /gene="hemC"
                     /locus_tag="PLZ72_11410"
     CDS             complement(879..1838)
                     /gene="hemC"
                     /locus_tag="PLZ72_11410"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNA36948.1"
                     /translation="MRVRIATRASPLARWQADHVAGLLRSADPDLDVEVVALSTEGDR
                     RTDVPLSEIGGKGVFATEVQAALLDGRADVAVHSAKDLPARTPDGLVIAAVPERGDVR
                     DALVGCRLVDLPTDGVVATGSARRRVQLAHLRPDLRFEDLRGNMATRIARSERDDVDA
                     VVVAAVALHRLGLADRLTDVLPATLMVPQVAQGALAVECRSDDAALRELLAGIEHAAS
                     RRAVDAERAFLSELGGDCTLPAGAHATILPTDDDAGPAASAGPVASGVLSIRGILGAD
                     PDAHGRVRVAHAVEVGSDPDAVGRALAVRLRADVDATAGEAPR"
     gene            complement(1845..3311)
                     /locus_tag="PLZ72_11415"
     CDS             complement(1845..3311)
                     /locus_tag="PLZ72_11415"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNA36949.1"
                     /translation="MARVKERQPGVSVVVVGANHRTAPLDLLERMAVSGERLPKLLHA
                     LDAGADTSEVVVLATCNRTEVYLVAERFHGAYAEVRDFFADLTFLPPERFAESLYVHY
                     DDAAVRHLFEVTAGLDSAVPGEHEILGQVGSAWELARQEGTARRGLNLLFRHALEVGK
                     RARTETSIARHVTSVSQAAVILAGERLAELRGITDAPDACTAAGARAGLAGARAVLIG
                     AGTMGRAAGSFLADAGLAEIVVANRGPERAEALVDRLRTDRDSGIARAVPLAELSTVM
                     ADADLVVAATGAPGAVLTAADVAPAVQGRSRPLVVVDIAMPRDVDPEVAALGGVELLD
                     MDAVAAFTEAGLAGRRREVAAVREIVDEEVVRFHGASNARRAAPVITALREQAECIRC
                     AEIDRLSGRLGELTDAQRDAVDAITRGLVAKLLHEPSVRLRAAAGSARGDRLADSVRE
                     LFALLDDPDDSDDPASGTASGTGIPDDSGAVVEPPSGG"
     gene            complement(3393..4058)
                     /locus_tag="PLZ72_11420"
     CDS             complement(3393..4058)
                     /locus_tag="PLZ72_11420"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNA36950.1"
                     /translation="MAPSGRASIPEATVARLPLYHRCLLDLRDEGRPTVSSEQLADLA
                     GVNAAKVRKDLSHFGSYGTRGVGYDVAHLLVEIASALNVDRDWPCVIVGAGNLGSALA
                     TFAGFTNRGFTVVGVLDVDPAKVGRPLGSLTVRHQDDLAELVAREGVAIGIVAVPPVA
                     AQEAADRLVEVGITSILNFAPTVLDVPDHVQVRDVDLAVELQILAFHEDPSRRVGPTR
                     TAG"
     gene            4229..5011
                     /locus_tag="PLZ72_11425"
     CDS             4229..5011
                     /locus_tag="PLZ72_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36951.1"
                     /translation="MSAAAAMGAVAVLVSVAFGLSTLERWLARRKPHELAWTVSLAMF
                     AVASLAYFSAAALGWGELNFRVFYLFGAILNVPWLALGTVYLLGGVHRGDVARWWLVA
                     LSAFCAGVVLATPLLGSLDGAGIPSGKEVFGVGPRIMAAVGSGVGAVVLIGGAAWSAV
                     GLLRVRRRPEVAAAMPIPPGRLALTNVFIAVGSLVLGSGGTMFGTGDQMVDFGIWLAA
                     GVTILFVGFLFSNPGRPAGEVAPTDPYWAEIYELATGPMEPA"
     gene            complement(5061..6554)
                     /locus_tag="PLZ72_11430"
     CDS             complement(5061..6554)
                     /locus_tag="PLZ72_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA36952.1"
                     /translation="MNLATIIDPHPGEATALISRGRETDYDTLRTQVAALRGALVEMG
                     LEPGERLAIIAGNNWYFVVSYLAALGAGLVAVPLNPTNPAVAMAHELSESGAVAIVAT
                     PTARAALREIDWSTLPDVRTFIGAGFEPGPLGVDIDTLLDHEPAPVVERAPDDVAVLI
                     FTSGTAGSPRPAMLTHANLLANIRQVLAADERVQEPTDVVFGLLPMFHIFGLNVMLGV
                     AMAVGASVLLVERFDPVSAIESVEKHGVTTIAGPPTMWAAFAGLPGVGGHEFDSVRRA
                     ISGAAKLPTEVAQAMEARYGVHLQEGYGLTEASPVVTAPISGVTPSGSVGIPVPGVEM
                     RLVDGDGDDVLVGDPGELLVRGPNVFAGYWNDQEATDRVITADGWLRTGDVGVVDDDG
                     NVYLVDRAKDLIIVSGFNVFPAEVEEVLLQHPAVEGVAVVGVPHPHTGESVKAYVKAR
                     DGVPIEEDDVIDFCAARLPHYKCPNKVWFVEDIPRGLGGKVIRRLLG"
     gene            complement(6748..7773)
                     /locus_tag="PLZ72_11435"
     CDS             complement(6748..7773)
                     /locus_tag="PLZ72_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNA36953.1"
                     /translation="MAADAPPDGSPRYDTVSFLSDYGHGDEFVGVVHSVIHQLAPGAT
                     VIDITHGIEPYDIRAAGLTLARSVQYLAPGVIMAVVDPGVGTHRRPIAVEVGSGRAVF
                     IGPDNGLLAPAVGLLGGATRAVWLNDAEHQLAAPGPLFDGRDVFAPAAAHLCNGVPLT
                     DLGEEVDTATLFPGILPISRVEDGALVAEVLWVDRYGNVQLNVDPDELDALAAEAPAD
                     RPTGSSERSGGGHFEVLLDGSRRTARRVVAFEDIAPGAVGLVVDSYGLISLAARQASA
                     AEDLGIGTGDGVTLRPIVDSDDTSDDAGDAGDGSAAESVTAVRVTFGRRPTPSDPAST
                     DVPGGAG"
     gene            7863..9014
                     /locus_tag="PLZ72_11440"
     CDS             7863..9014
                     /locus_tag="PLZ72_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HNA36954.1"
                     /translation="MESEGDIGVPAGGRAATTGRMVRGWRGVAVLASVVLAAAACAPD
                     PPPPPATAPVVSSFGALGEPHVEPALVPLRWGVTDPQGDAMSCRLDGDGDGTWDETID
                     PCPPDVSRNVSAVDAGAHTARLEVSDGTNTTLATATYHVGTRIDPEPFDIVIRPSGTV
                     DPDVLAAMQQAATRWERVVARGLGDVPVHLAAGSCGSGNAAFDGVVDDLVVDVSMKVI
                     PPAAWAAPCVDGPDGLPRFGFVEFNPNLTGALLDAGMLDEVAGHELGHVLGFGVTWQD
                     GRDVTGAGGTDPRFNGPRVRAENSLLRRSVDVPVMTVDGVMSPHWESVFLGPEIMATT
                     PDGSALSRLTIASLADIGYAVDIGAADPYSPPLPAGTCIAFSATVTRCW"
     gene            complement(9127..>9707)
                     /gene="proC"
                     /locus_tag="PLZ72_11445"
     CDS             complement(9127..>9707)
                     /gene="proC"
                     /locus_tag="PLZ72_11445"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNA36955.1"
                     /translation="AVDALATAAAAAPGLCRVLSIAAGVGTAALQAAVGDSVAVVRAM
                     PNTPALVGEGASAICAGPTAVDADLDWAEGLLGAVGTVVRVPERSMDAVTGLSGSGPA
                     YVFLVAEALIDAGVAAGLPRDVADRLTRQTLLGSARLLAESDDGPEALRAAVTSPGGT
                     TAAGLRQLESHAVRAAVIDAVAAATARSAELG"
BASE COUNT         1250 a         3449 c         3776 g         1232 t
ORIGIN      
        1 ccggaggccg cagccgcgat cgactggctc cgtcgccggg tcctcgacgg cgatggtcgg
       61 ggcgacgagc acctcggcgc cggcgtcgcg caggcggcgg gcgagctgcg gcgactggtc
      121 ccgggtccgg gtgacgacca cggtccggcc gagcaagggc cgccgctcga accagtccag
      181 gcgttccccg gcgacgtcgc cgatgacgat cgtggccggc gacgccaggt cgtggtcggc
      241 cagggtgctg agcgtggcgc ggacggtctg ctgggtgggt cgggtgcccc accggatggc
      301 cgcggcgggc gtgtcgggcg acaggccgcc ggcgaccagg cgttcggcga tgcggggcag
      361 gttgcgcacc cccatcagca cgacgatggt cccgcccacc cgggcgagcg cctcccagcc
      421 gacgtcggtg cggcccttgg acgggtcctc gtggccggtc acgatcgtga cgctggtcga
      481 cgagtagcgc aacgtgaccg ggatgccggc gtaggcgggg accgcgagcg cggaggtgat
      541 gccggggacg acctcgtagt cgacgccggc ggcggcgagc gcggcggcct cctcggcgcc
      601 gcgggcgaac acgaacgggt cgccgccctt gagccgcacc accgtctggc cccggcggcc
      661 gcgttcgacc agcagctcgt tgatggccga ctgcggggtg gacggcccgt cggggctctt
      721 gccgacgtcg atgagctcgg cgtccggcgg tgccaggtcg agcagctcgc gtgccgacag
      781 ccggtcgtgc acgacgacat cggcgcgggc cagcagctcg gcgccccgga cggtgatcag
      841 ccccgggtcg ccgggccccg cgccgacgag gtggacggtc accggggcgc ctccccggcg
      901 gtggcatcca cgtccgcgcg caggcggacg gccagggcac ggccgaccgc gtcggggtcg
      961 gagccgacct cgacggcatg cgcgacgcgg acgcgaccgt gggcgtcggg gtccgcgccg
     1021 aggatgcccc ggatgctgag cacgccggag gcgaccggac cggcagaggc tgcgggaccg
     1081 gcgtcgtcgt cggtgggaag gatcgtggcg tgggcaccgg cgggcagggt gcagtcgccg
     1141 cccagctcgg acaggaacgc ccgttcggcg tcgacggccc ggcggctggc ggcgtgctcg
     1201 atgccggcca ggagctcccg cagagccgcg tcgtcggagc ggcattcgac ggccaacgcg
     1261 ccctgggcga cctgcgggac catcagcgtg gcgggcagca cgtcggtgag gcggtcggcc
     1321 agacccaggc ggtgcaacgc caccgccgcg accaccaccg cgtcgacgtc gtcgcgctcg
     1381 gagcgggcga tgcgggtggc catgttcccc cgcagatcct cgaagcgcag gtcgggtcgc
     1441 aggtgcgcga gctgcacccg ccgacgggcc gagccggtgg cgaccacgcc gtcggtgggc
     1501 aggtcgacca gacggcaccc gacgagggcg tcgcggacat cgccgcgctc gggcaccgca
     1561 gcgatgacca gcccgtcggg ggtccgggcc gggaggtcct tggcggagtg caccgccacg
     1621 tcggcccgcc cgtcgagcag cgcggcctgc acctcggtgg cgaacacgcc cttcccgccg
     1681 atctccgaca gcgggacgtc ggtgcggcgg tcgccctcgg tgctgagcgc gaccacctcg
     1741 acgtcgaggt cggggtccgc ggagcgcagg agcccggcga cgtggtcggc ctgccaacgc
     1801 gccagcggcg atgcccgcgt ggcgatccgg acccgcacgg aggctcagcc cccggagggt
     1861 ggctcgacga cagcgccgga gtcgtcgggg atgccggtcc cgctggccgt cccgctggcg
     1921 gggtcgtcgg agtcgtcggg gtcgtcgagg agggcgaaca gctcgcgcac cgagtcggcc
     1981 aggcggtcgc cccgagccga ccccgccgcg gcgcgcagcc tcacgctcgg ttcgtgcagc
     2041 agcttggcca ccaagcccct ggtgatcgcg tcgacggcgt cgcgctgggc gtcggtcagc
     2101 tcacccagcc gcccggacag ccggtcgatc tcggcgcagc ggatgcactc ggcctgctcg
     2161 cgcagggcgg tgatgaccgg agcggcacgc cgggcgttgc tggcgccgtg gaaccgcacg
     2221 acctcttcgt cgacgatctc gcgcaccgcg gcgacctcac ggcgacgacc ggccagcccg
     2281 gcctcggtga aggctgcgac cgcgtccatg tcgagcagct cgaccccgcc gagcgcggcg
     2341 acctcgggat cgacgtcgcg gggcatggcg atgtcgacca cgacgagggg tcgcgaccgt
     2401 ccctgcaccg cgggggcgac atcggcggcg gtgagcaccg ctcccggtgc gccggtggcg
     2461 gcgacgacca ggtcggcgtc ggccatgacc gtcgacagtt cggccagcgg cacggcgcgg
     2521 gcgatgccgg agtcgcggtc ggtccgcagt cggtcgacca gggcctcggc ccgctcgggg
     2581 ccgcggttgg ccacgacgat ctcggccagg ccggcgtcgg ccaggaacga ccctgccgcc
     2641 cggcccatgg tcccggcgcc gatcagcacg gcgcgcgccc cggccaggcc ggcccgcgct
     2701 ccggccgcgg tgcacgcgtc gggggcgtcg gtgatgccgc gcagctcggc caggcgctca
     2761 ccggccagga tgaccgcggc ctgcgacacc gaggtgacgt gacgggcgat cgacgtctcg
     2821 gtgcgggccc gcttgccgac ctcgagcgcg tggcggaaca ggaggttgag gccccgtcgg
     2881 gcggtgccct cctggcgggc cagctcccag gccgagccga cctggcccag gatctcgtgc
     2941 tcgccgggca ccgcggagtc gaggccggcg gtcacctcga acaggtggcg gaccgcggcg
     3001 tcgtcgtagt ggacgtagag gctctcggcg aagcgctcgg gcggcaggaa ggtcagatcg
     3061 gcgaagaagt cccggacctc ggcgtacgcg ccgtggaagc gctcggccac caggtacacc
     3121 tcggtgcggt tgcaggtggc gagcacgacg acctcggagg tgtcggcgcc cgcgtcgagg
     3181 gcgtggagga gcttggggag gcgctcaccg ctgaccgcca tgcgctcgag gagatcgagc
     3241 ggggcggtgc gatggttcgc tccgacgacg acgacagaca cgcccggctg gcgctccttg
     3301 actcgggcca ccggggagga cggcgaccgc tgcggacgcc ctccagcatg ctgcgcgacc
     3361 ggggccggtc gccagttggt cgccgctcgc ggtcagcccg cggtgcgggt ggggccgacg
     3421 cggcgggacg ggtcctcgtg gaaggcgagg atctgcagct cgacggccag gtcgacgtcg
     3481 cggacctgga cgtggtcggg cacgtcgagc acggtcgggg cgaagttgag gatcgaggta
     3541 atgccgacct cgaccaggcg gtcggccgcc tcctgcgcgg cgacgggcgg gacggccacg
     3601 atcccgatcg cgacgccctc gcgggcgacc agctcggcca ggtcgtcctg gtggcgcacc
     3661 gtgagcgatc cgagcggacg cccgaccttg gccgggtcga cgtcgagcac gccgaccacg
     3721 gtgaagccgc ggttggtgaa gccggcgaag gtggcgagag cggagcccag gttgccggcg
     3781 ccgacgatga cgcagggcca gtcccggtcg acgttgaggg cgctggcgat ctcgaccagc
     3841 aggtgggcga cgtcgtagcc gaccccgcgg gtgccgtacg agccgaagtg cgacaagtcc
     3901 ttgcgcacct tggcggcgtt gaccccggcc aggtccgcga gctgctcgga ggacaccgtg
     3961 gggcggccct cgtcgcgcag gtcgagcagg cagcggtggt acagcggaag ccgcgccacg
     4021 gtcgcctcgg ggatggaggc tcgaccggag ggcgccatgg gtcgaccgta ccccgctcgt
     4081 gcgacttttc acaaagtgcc gggaggcccc ggcccgctcc gacggtcccg ccgatggccc
     4141 cgtcggctgc ctcgcccgac ggggtcggtc ggggacatgc tggggccgtg cccgaccccc
     4201 gcccgctccc gaccacctcg gagatccggt gagcgcggcc gcggcgatgg gcgccgtggc
     4261 ggtgctcgtg tcggtcgcgt tcgggctgtc gacgctggag cgctggctgg cccggcgcaa
     4321 gccccacgag ctggcctgga ccgtgtcgct ggccatgttc gcggtggcgt cgctggcgta
     4381 cttcagcgcg gcggccctgg gatggggcga gctgaacttc cgggtcttct acctgttcgg
     4441 cgccatcctc aacgtgccgt ggctggcgct cgggaccgtc tacctgctcg gcggcgtgca
     4501 ccgcggtgac gtcgcccgct ggtggttggt cgccctcagc gcgttctgcg ccggggtggt
     4561 gctcgccact cccctcctcg ggtcgctcga cggggccggg atcccgtcgg gcaaggaggt
     4621 cttcggcgtc ggcccccgga tcatggccgc cgtcggctcc ggcgtgggtg ccgtggtcct
     4681 catcggcggc gcggcgtggt cggcggtcgg gttgctgcgg gtgcggcgcc ggcccgaggt
     4741 ggccgcggcg atgccgatcc ctccggggcg cctggctctc accaacgtgt tcatcgccgt
     4801 cggctcgctg gtcctgggat ccggcgggac gatgttcggc accggcgacc agatggtcga
     4861 cttcggtatc tggctggcgg cgggcgtgac gatcctgttc gtcgggttcc tgttctcgaa
     4921 cccgggccga cccgccggcg aggtcgcccc gaccgatccg tactgggccg agatctacga
     4981 gctggccacc ggcccgatgg agccggcctg acgggatctc cgggacccgg cggtcggggc
     5041 ggcggtcccg ctggcggccc tcagccgagg agccggcgga tcaccttgcc gccgaggccg
     5101 cgggggatgt cctcgacgaa ccacaccttg ttcgggcact tgtagtgggg caggcgcgcc
     5161 gcgcagaagt cgatgacgtc gtcctcctcg atggggacgc cgtcgcgggc cttgacgtag
     5221 gccttgaccg actcgccggt gtgggggtgc gggacgccga ccacggccac gccctcgacc
     5281 gcggggtgct gcaacagcac ctcttcgacc tcggcgggga acacgttgaa gcccgacacg
     5341 atgatcaggt ccttggcccg gtcgaccagg tagacgttgc cgtcgtcgtc gaccacgccg
     5401 acgtcgccgg tgcgcagcca gccgtccgcg gtgatgaccc ggtcggtcgc ctcctggtcg
     5461 ttccagtagc cggcgaacac gttcggaccc cgcaccagca gctcgccggg gtcgccgacc
     5521 aacacgtcgt cgccgtcgcc gtcgaccagg cgcatctcga ctccggggac cgggatgccg
     5581 accgagcccg acggggtgac gccggagatg ggggcggtga ccaccggcga cgcctcggtg
     5641 agcccgtagc cctcctggag gtgcacgccg tatcgcgcct ccatggcctg ggcgacctcg
     5701 gtcggcagct tggcggcgcc ggagatcgct cggcgcaccg agtcgaactc gtggccaccc
     5761 accccgggaa gcccggcgaa ggcagcccac atggtggggg gaccagcgat ggtggtgacg
     5821 ccgtgcttct cgacggactc gatggccgac accgggtcga agcgctcgac gagcaggacc
     5881 gacgcgccga ccgccatcgc cacgcccaac atgacgttga gcccgaagat gtggaacatc
     5941 ggcagcaggc cgaacacgac gtcggtcggc tcctggaccc gctcgtcggc ggccagcacc
     6001 tgacggatgt tggccagcag gttggcgtgg gtgagcatgg cgggacgggg cgaccctgcc
     6061 gtgcccgagg tgaagatcag cacggcgacg tcgtcggggg cgcgttcgac gaccggtgcc
     6121 ggttcgtggt cgagcagcgt gtcgatgtcg acgcccagcg gccccggctc gaacccggcg
     6181 ccgatgaagg tgcgcacgtc ggggagggtg gaccagtcga tctcgcgcag ggccgcccgg
     6241 gcggtcggcg tggccacgat cgccaccgcg cccgactcgc tgagctcgtg cgccatcgcc
     6301 accgccgggt tggtcgggtt gagcggcacg gcgaccagtc cggcgccgag ggcggccagg
     6361 tacgacacga cgaagtacca gttgttgccg gcgatgatgg cgaggcgctc gccgggctcc
     6421 aggcccatct cgaccagggc gccgcgcagg gccgccacct gggtgcgcag cgtgtcgtag
     6481 tcggtctccc gaccccggct gatcagcgcg gtggcctcgc cgggatgggg atcgatgatc
     6541 gtcgccaggt tcaccgccgg cgacgatatc cgctcgtcac ggcgttccga cgcacgaagg
     6601 ccgccggtgt ggccgccggt gggtcgccgg tgagcggccg ccgcggcgag ggcggccccg
     6661 gtcagcgggc caggacgacg agctggagga acgcggcgcc gacgatcacc gccatgagga
     6721 cgatgacggt gatggtggtg aagcgtctca tcccgcacct ccggggacgt cggtcgaggc
     6781 tgggtccgac ggggtcggtc gtcgaccgaa ggtgacccga accgcggtga ccgactcggc
     6841 cgcggacccg tcgccggcgt cgccggcgtc gtcggaggtg tcgtcggagt cgacgatcgg
     6901 gcgcagggtg acgccgtcgc cggtgccgat gcccaggtcc tcggccgcgc tggcctgacg
     6961 ggcggcgagc gagatcaacc cgtagctgtc gacgaccagg ccgacggcgc ccggggcgat
     7021 gtcctcgaac gccaccaccc ggcgggcggt gcgacgggat ccgtcgagca gcacctcgaa
     7081 gtgcccgccc ccggagcgct cgctgctgcc ggtcggccgg tcagccggcg cctctgcggc
     7141 cagggcgtcg agctcgtcgg ggtcgacgtt gagctgcacg ttgccgtagc ggtcgaccca
     7201 gagcacctcg gcgaccaggg ccccgtcctc gacccgggag atgggcagga tccccgggaa
     7261 cagcgtggcg gtgtcgacct cctcgccgag gtcggtgagc ggcaccccgt tgcacaggtg
     7321 ggcggccgcg ggggcgaaga cgtcgcgtcc gtcgaagagc gggccgggag cggcgagctg
     7381 gtgctcggcg tcgttgagcc acaccgcccg ggtggcgccg ccgagcagtc cgaccgccgg
     7441 ggcgagcagg ccgttgtcgg gaccgatgaa gacggcccgg ccgcttccga cctcgaccgc
     7501 gatcggccga cggtgggtgc cgacgccggg gtcgaccacc gccatgatca cgccgggtgc
     7561 caggtactgg acgctgcggg ccagggtgag gccggcggcg cggatgtcgt agggctcgat
     7621 gccgtgggtg atgtcgatga cggtggcgcc gggcgcgagc tggtggatca ccgagtgcac
     7681 gacgccgacg aactcgtcgc cgtggccgta gtcggacagg aacgagacgg tgtcgtagcg
     7741 gggggacccg tcggggggag cgtcggcggc cacgggcccc gaacctaccg ctccgggctc
     7801 accggctccg aaggtgaccc cagaccgtgg accgaccccg tggtcggtgg tcgaatgggg
     7861 acatggagtc cgagggcgac atcggggttc cggcgggcgg ccgggccgcc accaccggcc
     7921 ggatggtccg aggctggcgc ggcgtggccg tgctcgcctc ggtcgtgctg gccgctgcag
     7981 cctgcgcccc ggacccgcca cctccgccgg ccaccgcgcc ggtggtctcg tcgttcgggg
     8041 ctctcgggga accccacgtc gaaccggcgc tcgtcccgct ccgctggggg gtgaccgacc
     8101 cccagggcga cgccatgtcc tgccggctgg acggcgacgg ggacgggaca tgggacgaga
     8161 cgatcgaccc ctgccctccc gacgtcagcc gcaacgtttc cgcggtcgac gccggcgccc
     8221 acaccgcccg actcgaggtc agcgacggga cgaacaccac gctcgccacc gcgacctacc
     8281 acgtcggcac ccgcatcgac cccgagccgt tcgacatcgt gatccggccg tcgggcacgg
     8341 tcgaccccga cgtcctggcg gcgatgcagc aggcggcgac gcgttgggag cgtgtcgtcg
     8401 cgaggggact gggcgacgtt ccggtccacc tcgcggccgg gtcctgcggc tcgggcaacg
     8461 ctgcgttcga cggggtggtc gacgacctgg tggtcgacgt gtcgatgaag gtgatccccc
     8521 cagccgcgtg ggcggcgccg tgcgtcgacg gacccgacgg cctcccccgg ttcggcttcg
     8581 tcgagttcaa cccgaacctg accggtgccc tgctcgacgc cggaatgctc gacgaggtcg
     8641 cggggcacga gctgggccac gtgctcggct tcggggtcac ctggcaggac ggtcgggacg
     8701 tcaccggggc gggcggaacc gacccccggt tcaacgggcc ccgggtccgg gccgagaact
     8761 cgctcctgcg acgatcggtc gacgtccccg tcatgaccgt cgacggagtc atgagcccgc
     8821 actgggagtc ggtgttcctc ggtccggaga tcatggccac caccccggac ggcagcgcgc
     8881 tgtcacgcct gacgatcgcg tcgttggccg acatcggcta cgcggtcgac ataggggcgg
     8941 cggatccgta ctcgccaccg ctgccggcgg ggacgtgcat cgcgttctcc gcgacggtca
     9001 cgcgatgctg gtgagccatc cgccaacgcc gacgccccga cgggacgcgt aggaccgcgg
     9061 ttcgcagacc cgctcttcgc agacccgcgg tcaacaggac cgtgggccgg ggccccggcc
     9121 cggcgctcag ccgagctcgg cggaacgggc cgtcgcggcg gcgacggcgt cgatcaccgc
     9181 ggcgcgcacc gcgtgggact cgagctggcg caggcccgcc gcggtggtgc cgcccggcga
     9241 ggtgaccgcg gcgcgcagcg cctcggggcc gtcgtcggac tcggcgagca gacgggccga
     9301 gccgagcagg gtctggcggg tcagtcggtc cgccacgtcc cgcggcaacc cggcggcgac
     9361 accggcgtcg atcagcgctt cggcgacgag gaacacgtag gcgggacccg atcccgacag
     9421 tccggtcacc gcgtccatcg agcgctccgg gacccgcacg acggtgccga ccgcgccgag
     9481 cagcccctcg gcccagtcca ggtcggcgtc gacggcggtg ggtccggcgc agatcgcgga
     9541 cgccccctcc cccaccagcg ccggcgtgtt gggcatggcc cgcacgacgg cgaccgagtc
     9601 gccgaccgcg gcctgcagcg cggcggtccc cacccccgcg gcgatcgaca gcacccgaca
     9661 caggccggga gcagccgcgg cggccgtggc cagggcgtcg accgcgt
//
LOCUS       DAOQOD010000120         9705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2564_length_9705_cov_12.332435, whole genome shotgun sequence.
ACCESSION   DAOQOD010000120 DAOQOD010000000
VERSION     DAOQOD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2564_length_9705_cov_12.332435"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLZ72_11450"
     CDS             complement(<1..441)
                     /locus_tag="PLZ72_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36956.1"
                     /translation="MVDRGSDISTTEEQISGAVSSAHADALAELSRPDPPESLWERVV
                     TTLVPLARSSPGWLRVVVATVVVAAVAGGWVWLRRPVDPVAGLPRVSDPVATSSTALE
                     PTAASVTGSTAAGALTTGSVTVHVAGAVQLPGVVTLPAGSRAVDA"
     gene            complement(560..2053)
                     /locus_tag="PLZ72_11455"
     CDS             complement(560..2053)
                     /locus_tag="PLZ72_11455"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNA36957.1"
                     /translation="MTATTISHWIGGTRSPGGSDRHGDVFNPATGSVSATVPFATSSE
                     VDAAVAVAAEAFLSWRDVSLSRRTEIMFRFRELLSAHKDDLAAAVTAEHGKVLSDAAG
                     EVQRGIENVEFACGAPHLLKGSMSHQVATRVDVHSLLQPLGVVAGISPFNFPAMVPLW
                     MFPNALVCGNTFVLKPSEKDPSASVLMAELLTEAGLPDGVFNVVHGDKEAVDALLVNP
                     TVQAVSFVGSTPIARYIYETGTSHGKRVQALGGAKNHMVVLPDADLGMAADAAVSAAY
                     GSAGERCMAISVVLAVGDVADPLVAAIAERARAVRIGPGTDADSEMGPLVTREHRDKV
                     ASYLPAASEAGARLVLDGREQSFDGDGFFLGVSIIDDVTTDMDCYRDEIFGPVLAVVR
                     VPDFDAAMAVLEDNPYGNGTAIFTRDGGAARRFVDEVPVGMVGVNVPVPVPVAAYSFG
                     GWGDSLFGDSHMYGPEGLAFYTRSKVVTSRWPDPSTSTVDLGFPQSR"
     gene            complement(2133..2834)
                     /gene="trxA"
                     /locus_tag="PLZ72_11460"
     CDS             complement(2133..2834)
                     /gene="trxA"
                     /locus_tag="PLZ72_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA36958.1"
                     /translation="MADVTDATFTTDVVERSRTVPVVVDLWAEWCGPCRTLGPILEKV
                     VADAGGQVELAKVDIDANPSVAAMFQVQSIPAVYAMKDGKVVDGFLGAQPEWQVAEFV
                     QRLYPSVEDREVDRLVAAGDEASLRAALELEPSEETVIVALAELLVSDGRAEEGLALL
                     ERIPESAATRRVAALARTGGLADAGDLDARLDSLLSQVKDDDDARQQFVDLLELIEDP
                     DAAGAWRRKLAARLF"
     gene            3022..4101
                     /locus_tag="PLZ72_11465"
     CDS             3022..4101
                     /locus_tag="PLZ72_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNA36959.1"
                     /translation="MTEASEVTDDIETVPAGIDADGVTTWFADHVVDVAPPLRFELIA
                     GGHSNLTFSVTDRDARRWVLRRPPLRQVLATAHDMGREHRIISALGPTDVPVPPAVGL
                     CTDESVNGVPFYVMDFVDGRVVRGAADAAALSPAQRRTAGESLVDVMAAIHAVDVDAV
                     GLGDLGRKDDYIARQLKRWYGQFQASDSQVDGGLGLPEVDRIHDRLAAAIPPQQGSAI
                     VHGDYRLDNCMLADDGSVKAVLDWEICTLGDPLADLGLLWVYWSDPDQAAVLPQASPT
                     ALEGFPRKAELIERYAAGSQRDLSQLDYYIAFGYWKLTCIIAGVYARYAGGAMGDVPA
                     DQVQGFRSMLDALSDMTVTAADGIG"
     gene            4103..4996
                     /locus_tag="PLZ72_11470"
     CDS             4103..4996
                     /locus_tag="PLZ72_11470"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNA36960.1"
                     /translation="MSSLVHRELNPELTDPVLVVGLEGWIDSGLGAVNAVSAVRAGAD
                     AEVVATFDADRLLDHRARRPIMHLVNGLITNLAWPSIELLAGTDAHGHDLLLLVGAEP
                     DFEWRAFSEAVLDLALEFGCRMVVGLGAYPAPVPHTRDVLLSVTTSSAKLSDDLHGYV
                     RGTLDVPGGIQTVIDVDANRAGLPALGLWAQVPHYVSTMPYPAASLALLDGLADVAGI
                     DAHRGQLAADAAATRIRIDELVAENPQHQAMVTQLEEFVDQGLADGPVDAPTIDFERI
                     PSGDELAAELQEFLRQRPDED"
     gene            complement(5038..6750)
                     /locus_tag="PLZ72_11475"
     CDS             complement(5038..6750)
                     /locus_tag="PLZ72_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA36961.1"
                     /translation="MKTMEDPQGSGSSPLRRWGPLIAVVAVIAVVVGLLVTRGGGDDT
                     SASTTTTPSGGTDLPEGVVSFSAAKADGTVDDIDWGKRCDTDTGVLAIPIFPAPECYK
                     PFTGDNGGETTVGVTADTIKLVVYLPQENDPILSFIYKQVGNTDTPTQLFESYQGYNE
                     MLATYYETYGRKVELVRYDATGNIQDEVAATADAETIARDIKPFAVLGGPSLTEAFGD
                     TLAANKITCVSCTPGQPGQWYLDRAPYIWDIQKNTDQNLQMVAEYLGKRVAHHPAAYG
                     GDAVKGKDRKLGLIALSSSPQSDEMRQKFLDDLKKNYDTEFAQVATYTDPVALAGQAG
                     EMMAKMKSDGITSVVFIGDPLAPQTLTKNATEQGFFPEWIVTGSALVDTTIFARTYDQ
                     SQWAHAFGPSNLFARSSGNLTGPTFLYKWFFGKNPPAEQVALLLPNLQLFYAAFQGIG
                     PNLSPEMLREALFRAPIVKSTVLTPQISWGDHDIWPTTDYAGLDDQTEIFWNPTATGP
                     DELGKDGTGMWAYVNGGERYLPGQWPDSAAKVFADAPDPVTFYTQLPEGITLPTYEPL
                     KPAG"
     gene            complement(6773..>9705)
                     /locus_tag="PLZ72_11480"
     CDS             complement(6773..>9705)
                     /locus_tag="PLZ72_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA36962.1"
                     /translation="ATLAHPIRAVGEVAGSGPVYALLILFGLNCVDELDRTGFGILLP
                     NVRDAFGMSNTGILTLVALTLLGAFLMQMPIAVWADRGNRVFITLLGAATWAAFSIMT
                     GLSTAIWMLIVARAGAGIGRAVVDPTHASLLSDWFPVDRRPAVFSFHRAANVLGQFLG
                     PLLAGGIAYLTDWRVPFLVFAVPTVILVVLGTRMKEPIRGAQERRASGASDEVVETEE
                     PIPSFAESWRLLWKIEVLRRIWYAVPFLAVSLIGFVSLASLLYSDVYRLDELQRGYLA
                     AVVEPFQLLGLAVGARLGTRLFLKSPALIFRLLRWVSFVAAALAAGFAYAPNIAVAVV
                     FNVLLTADLAILLPGILATLSLAIPARARAVGFSVASWWAIPGLALLPLIGWIGDNWG
                     TRLGMLVMTPILLVGGLMISSGGSVIQRDIEDVWKSSTARSQALLERRAGRSKLLLVR
                     DLNVGYDGLQVLFDVNMEIAEGEIVALLGTNGAGKSTLLRAITGITEADFGAVIFDGR
                     DITHAPPNEIAALGVASMPGGAGVFPGLSVRENLRSAGWLVRRDRPELDARTAEVLEV
                     FPVLAERLEEPASNLSGGQQQMLALAMALLSRPKLLIIDELSLGLAPVVVGKLAELVR
                     EVAAGGTTVLLVEQSVNVALGMAGTAYFMERGRVQFSGPAEELLNRPDLLRAVFLAES
                     VAAAGANDGANGGNGTGPDTRPAEPPINADPALELVAVTRRFGGINAVDQVSLSVAPG
                     EIVGLIGQNGAGKTTILDLISGFQPLDGGRILLNGHDLSHLSAAQRARHGLGRTFQGG
                     RLFSGLTVAETVAVSLDRSVAVKDPINAALRLPAAHDSEVAVGRRVRQLLELFGLMDY
                     RDSFTAELSTGTRRIVELACVVGHQPSVLLLDEPAGGVAQKEVEQLGALLGRIRSELG
                     CAMLVIEHDMPLVAGLANRLVALETGAVIAEGTPTDVLADPRVIASYLGDDHLAVARS
                     GHTN"
BASE COUNT         1415 a         3440 c         3470 g         1380 t
ORIGIN      
        1 cgcgtcgacg gctcggctgc ccgccggcaa ggtcaccaca cccgggagct gcaccgcgcc
       61 ggcgacgtgc accgtcaccg atcccgtcgt gagcgctccg gccgcggtcg acccggtcac
      121 cgacgccgcg gtcggctcca gcgcggtgct ggaggtcgcg accggatcgg acacccgtgg
      181 cagacctgcg acagggtcga cgggccgacg gagccacacc caccctcccg cgaccgccgc
      241 cacgaccacc gtcgccacga ccacccggag ccaaccgggc gacgaacggg cgagcgggac
      301 gagcgtggtg accacgcgct cccagagcga ctcgggtgga tccggacggc tcagctcggc
      361 cagcgcgtcg gcgtgcgccg acgagacggc tccgctgatc tgctcctcgg tcgtgctgat
      421 gtcgctcccg cggtcgacca cgcccactcc cccgtcgtgc cggcgcgcgc cggacgtgct
      481 gggggaagcg acgtcgaccc taggaccccg ggcggccctc cgtcaccggg gaccgctccc
      541 cggtgacggt gcgtcgcgat cagcgactct gcgggaagcc caggtcgacg gtgctggtcg
      601 aggggtccgg ccagcgcgac gtgaccacct tcgagcgggt gtagaaggcc agcccctcgg
      661 ggccgtacat gtggctgtcg ccgaacagcg agtcgcccca gcccccgaag gagtaggccg
      721 ccaccggcac cggcaccggc acgttgacgc cgaccattcc gacgggcacc tcgtcgacga
      781 agcgccgggc cgcgcctccg tcgcgggtga agatggcggt gccgttgccg tacgggttgt
      841 cctcgagcac cgccatggcc gcgtcgaagt cggggacccg taccaccgcg agcaccggtc
      901 cgaagatctc gtcccggtag cagtccatgt cggtcgtgac gtcgtcgatg atcgacacgc
      961 cgaggaagaa cccgtcgccg tcgaaggact gctcgcggcc gtcgagcacg aggcgcgccc
     1021 cggcctcgga cgcggcgggg aggtagctcg ccaccttgtc tcggtgctcc cgggtgacca
     1081 gcggtcccat ctcggagtcc gcgtcggtcc cggggccgat gcgcaccgcc cgggcccgct
     1141 cggcgatggc cgcgaccagc gggtcggcca cgtcgcccac cgcgaggacg accgagatcg
     1201 ccatgcaccg ctcgcccgcc gatccgtacg cggcggacac cgcggcgtcg gcggccatgc
     1261 ccaggtccgc gtcggggagc acgaccatgt ggttcttggc accgccgagc gcctggaccc
     1321 gcttgccgtg cgacgtgccc gtctcgtaga tgtagcgggc gatcggcgtg gagccgacga
     1381 agctgaccgc ctgcaccgtc gggttgacca ggagggcgtc gaccgcctcc ttgtcgccgt
     1441 gcacgacgtt gaacaccccg tcgggcagcc cggcctcggt cagcagctcg gccatcagca
     1501 ccgacgcgga cgggtccttc tccgacggct tgaggacgaa ggtgttgccg cacacgagcg
     1561 cgttggggaa catccacagc ggcaccatgg ccgggaagtt gaacgggctg atcccggcca
     1621 ccactccgag cggctgcagc agggagtgga cgtcgacgcg ggtcgcgacc tggtggctca
     1681 tcgagccctt cagcaggtgg ggggctccgc aggcgaactc gacgttctcg atgccgcgct
     1741 ggacctcgcc ggcggcgtcg gagaggacct tgccgtgctc ggcggtgacc gccgcggcca
     1801 ggtcgtcctt gtgggccgac agcagctccc ggaaccggaa catgatctcg gtccggcggc
     1861 tcagcgacac gtcacgccag ctgaggaacg cctccgcggc cacggccacc gccgcgtcga
     1921 cctcggagga ggtggcgaac ggcacggtgg cgctgaccga accggtcgcg gggttgaaca
     1981 cgtcgccgtg gcggtccgaa ccgccggggc tccgggtacc gccgatccag tgggagatgg
     2041 tcgtggcggt catggatatc ggtgtcctgt ctcgggcgac ccggacgggc cgcgtgtcat
     2101 gggtccgacc ggtggggccg ggcaccgccg gttcagaaga gccgtgcggc gagcttgcgg
     2161 cgccaggcgc cggcggcgtc gggatcctcg atcagctcga gcaggtcgac gaactgctga
     2221 cgggcgtcgt cgtcgtcctt gacctgcgag aggagcgagt cgagtcgggc gtcgaggtcg
     2281 ccggcgtcgg ccagccctcc ggtgcgcgcc agcgccgcga cccgacgggt ggcggccgac
     2341 tcggggatac gttcgagcag ggccaggccc tcttcggccc ggccgtcgga cacgagcagc
     2401 tcggccaggg cgacgatcac cgtctcctcg gagggctcca gctcgagagc ggcacgcagc
     2461 gacgcctcgt cacccgccgc gaccaggcgg tcgacctcac ggtcctcgac gctggggtag
     2521 aggcgctgca cgaactcggc gacctgccac tcgggttggg cccccaggaa gccgtcgacg
     2581 accttgccgt ccttcatggc gtacaccgcc gggatcgact ggacctggaa catcgcggcc
     2641 accgacgggt tggcgtcgat gtcgaccttg gccagctcga cctggccgcc ggcgtcggcg
     2701 acgaccttct ccaggatcgg gcccagcgtc cgacacggcc cgcaccactc cgcccacagg
     2761 tcgacgacca ccggcaccgt gcgggagcgc tcgacgacgt cggtggtgaa ggtggcgtcg
     2821 gtgacatcgg ccatgggacg acggagctcc tggtgtgcgt gcgggtgggt ggtggaggcg
     2881 cgtggcgtgg ccgtgacggc tgctcggcac cgcgtcggct gacccgggga gtctacgacg
     2941 gcgaccctcc gcgggcccgt ccggggccga caccgtcgtg tcgacgcccg cagtagcgtg
     3001 gcgaccgacc cagggggacg tgtgaccgaa gcatccgagg tgaccgacga catcgagacc
     3061 gtgcccgccg gcatcgacgc cgacggggtg acgacgtggt tcgccgacca cgtcgtcgac
     3121 gtcgcaccac cgctgcggtt cgagctgatc gcgggcggcc actccaacct gaccttctcg
     3181 gtgaccgacc gcgacgcccg acgctgggtg ctgcgccgac caccgctgcg ccaggtcctg
     3241 gccaccgccc acgacatggg gcgggagcac cgcatcatct ccgcgctcgg ccccaccgac
     3301 gtgccggttc ccccggcggt ggggctctgc accgacgagt cggtcaacgg cgtgccgttc
     3361 tacgtcatgg acttcgtcga cggtcgggtg gtccgcggtg ccgcggatgc ggcggcgctg
     3421 agcccggccc agcgccgcac cgcaggcgag tcgctcgtcg acgtcatggc cgccatccac
     3481 gccgtcgacg tcgacgcggt cggcctgggc gacctgggcc gcaaggacga ctacatcgcc
     3541 cgccagctca agcgctggta cggccagttc caggcgtcgg actcccaggt cgacggcggg
     3601 ctgggactgc ccgaggtcga ccggatccac gacaggctgg ccgccgcgat ccctccgcag
     3661 cagggctcgg ccatcgtgca cggcgactac cggctcgaca actgcatgtt ggccgacgac
     3721 gggtccgtga aggcggtgct cgactgggag atctgcacgc tcggcgatcc gctcgcggac
     3781 ctcggtctgc tctgggtgta ctggtccgac ccggaccagg ccgcggtgct gccccaggcg
     3841 tcgccgaccg ctctcgaggg gttcccccga aaggccgagc tgatcgaacg gtacgccgcg
     3901 ggctcgcagc gggacctgtc gcagctcgac tactacatcg ccttcgggta ctggaagctg
     3961 acctgcatca tcgccggggt ctacgcccgc tacgccgggg gcgccatggg cgacgtgccg
     4021 gccgatcagg tgcaggggtt ccgctccatg ctcgacgccc tgtcggacat gacggtgacc
     4081 gccgcggacg ggatcggctg acgtgtcctc gctcgttcac agggagctca accccgagct
     4141 gaccgacccc gtcctcgtcg tcgggctcga gggctggatc gactccggcc tcggcgccgt
     4201 caacgccgtc tccgcggtgc gcgccggcgc ggatgccgag gtcgtcgcca ccttcgacgc
     4261 cgaccggctg ctcgaccacc gtgcccgccg tccgatcatg cacctcgtca acggcctcat
     4321 caccaacctg gcgtggccgt cgatcgagct cctggccggc accgacgccc atggccacga
     4381 cctgctgctg ctcgtgggcg ccgagcccga cttcgagtgg cgggcgttct ccgaggcggt
     4441 gctggacctg gcgctcgagt tcgggtgccg catggtcgtc ggactgggcg cctatcccgc
     4501 ccccgtaccc cacacccgcg acgtgctgct gtcggtcacc acgtcgtcgg ccaagttgtc
     4561 cgacgacctg cacggctacg tgcgggggac gctggacgtg cccggcggga tccagaccgt
     4621 catcgacgtg gacgccaacc gggccgggct ccccgccctg gggttgtggg cccaggtccc
     4681 ccattacgtg tcgaccatgc cctaccccgc ggcgagcctg gcgctgctcg acgggttggc
     4741 cgacgtggcc ggcatcgacg cgcaccgcgg ccagctggcc gcggacgcgg ccgccacccg
     4801 gatccgcatc gacgagctgg tggccgagaa cccccagcac caggcgatgg tcacccagct
     4861 cgaggagttc gtcgaccagg gcctggccga cgggcccgtc gacgccccga cgatcgactt
     4921 cgagcgcatc ccctccggcg acgagctggc cgcggagctg caggagttcc tccgccagcg
     4981 tcccgacgag gactgatctc ccacggcccc gcggccccgg agcgggggcc tggcggctca
     5041 gcccgcgggc ttcaggggtt cgtaggtcgg cagcgtgatg ccctcgggca gctgcgtgta
     5101 gaaggtcacc gggtcggggg catccgcgaa caccttcgcc gcggagtccg gccactgacc
     5161 cggcaggtac cgctcgccgc cgttcacgta ggcccacatc ccggtcccgt ccttgccgag
     5221 ctcgtcgggg ccggtcgcgg tcgggttcca gaagatctcg gtctggtcgt cgaggccggc
     5281 gtagtcggtg gtcggccaga tgtcgtggtc accccagctg atctgcgggg tcagcacggt
     5341 gctcttgacg atcggcgcac ggaacagggc ttcgcgcagc atctccggtg agaggttcgg
     5401 cccgatgccc tggaaggcgg cgtagaacag ctggaggttg ggcagcagca gggcgacctg
     5461 ttcggcgggc gggttcttgc cgaagaacca cttgtagagg aacgtcggcc cggtcaggtt
     5521 cccgctggac cgggcgaaca ggttcgacgg cccgaacgcg tgggcccact gcgactggtc
     5581 gtaggtgcgg gcgaagatcg tggtgtcgac cagagccgag ccggtcacga tccactcggg
     5641 gaagaacccc tgctcggtcg cgttcttggt cagcgtctgc ggcgccagcg ggtcgccgat
     5701 gaagacgacc gaggtgatcc cgtcggactt catcttggcc atcatctcgc cggcctggcc
     5761 ggcgagggcg accgggtcgg tgtaggtggc gacctgggcg aactcggtgt cgtagttctt
     5821 cttcaggtcg tcgaggaact tctgtcgcat ctcgtcggac tgcgggctgg aggagagggc
     5881 gatcaggccg agcttgcggt ccttgccctt gacggcgtcg ccgccgtacg ccgcgggatg
     5941 gtgtgccacc cgcttgccca ggtactcggc gaccatctgg aggttctggt cggtgttctt
     6001 ctggatgtcc cagatgtagg gggcccggtc gaggtaccac tgcccgggtt gacccggcgt
     6061 acacgacacg caggtgatct tgttcgcagc gagcgtgtcg ccgaacgcct cggtgaggct
     6121 gggcccgccg agcacggcga acggcttgat gtcgcgggcg atcgtctcgg cgtccgcggt
     6181 ggccgccacc tcgtcctgga tgttgccggt ggcgtcgtag cgcaccagtt cgaccttgcg
     6241 gccgtaggtc tcgtagtagg tggcgagcat ctcgttgtag ccctggtagc tctcgaacag
     6301 ctgggtcggg gtgtcggtgt tgccgacctg cttgtagatg aagctcagga tcgggtcgtt
     6361 ctcctggggg aggtagacga ccagcttgat cgtgtcggcg gtgacgccga cggtggtctc
     6421 gccgccgttg tcgccggtga acggcttgta gcactccggc gccgggaaga tcgggatggc
     6481 gagcacgccg gtgtcggtgt cgcagcgctt cccccagtcg atgtcgtcga cggtgccgtc
     6541 ggccttcgcg gccgagaacg acacgacccc ctcgggcagg tccgtgcccc ccgatggtgt
     6601 ggtcgtcgtg ctcgccgagg tgtcgtcacc gccgccgcgg gtcaccagca gcccgaccac
     6661 gacggcgatc accgccacca ccgcgatcag cgggccccac ctgcgcagcg gactggagcc
     6721 ggacccctgg ggatcttcca tggtcttcat gtgcttcctc ctgttgcgcg gttcagttcg
     6781 tgtggccgga tcgggcgacg gccaggtggt cgtcgcccag gtaggacgcg atcacccggg
     6841 ggtccgcgag cacgtcggtc ggcgtgccct cggcgatcac cgccccggtc tcgagcgcga
     6901 cgagccggtt ggccaatccg gccaccagcg gcatgtcgtg ctcgatgacc aacatggcgc
     6961 agcccagctc gctgcggatg cggcccagca gggcgccgag ctgctcgacc tccttctggg
     7021 ccacgccgcc ggcgggttcg tcgagaagca gcaccgaggg ctggtggccg accacgcatg
     7081 ccagctcgac gatccggcgg gtgccggtgg acagctcggc ggtgaagctg tcgcggtagt
     7141 ccatcagtcc gaacagctcg agcagctgtc ggacccgccg gccgaccgcc acctcgctgt
     7201 cgtgcgcggc gggcaggcgc agcgcggcgt tgatcgggtc cttgaccgcg acagaccggt
     7261 cgagggacac cgcgaccgtc tcggccacgg tcaaccccga gaacaggcgc ccaccctgga
     7321 aggtgcggcc gagcccgtgg cgcgcccgct gcgctgccga caggtgcgac aggtcgtggc
     7381 cgttcaggag gatgcgtccg ccgtcgaggg gttggaagcc gctgatcagg tcgaggatcg
     7441 tggtcttgcc ggcgccgttc tggccgatca ggccgacgat ctcgccgggg gcgacgctca
     7501 ggctgacctg gtcgacggcg ttgatccccc cgaagcgccg ggtgaccgcg accagctcca
     7561 acgccgggtc ggcattgatc ggcggctcag cgggccgggt gtccggtccg gtgccgttcc
     7621 cgccgttcgc cccgtcgttg gccccggctg cggcgaccga ctccgccagg aacaccgccc
     7681 gcaggaggtc cggacggttc agcagctcct cggcagggcc ggagaactgc acccggcccc
     7741 gttccatgaa ataggcggtg ccggccattc cgagcgcgac gttcaccgac tgctcgacca
     7801 acagcaccgt cgtcccgccg gccgccacct cgcgcaccag ctcggcgagc ttgccgacca
     7861 ccaccggcgc caggccgagg ctcagctcgt cgatgatcag gagcttgggt cgcgacaaca
     7921 gcgccatcgc cagggcgagc atctgctgct ggccacccga caggttcgac gcgggttcct
     7981 ccagccgctc ggccagcacg gggaacacct cgagcacctc ggccgtccgg gcgtcgagct
     8041 cagggcggtc gcggcgcacc agccaccccg cggaccgcag gttctcgcgc acgctgagcc
     8101 cggggaacac cccggcgcca ccgggcatgg acgcgacgcc gagcgccgcg atctcgttcg
     8161 gtggggcgtg ggtgatgtcg cgtccgtcga agatgacggc cccgaagtcg gcctcggtga
     8221 tcccggtgat cgcccgcagc agggtcgact tgccggcgcc gttggtgccg agcagggcga
     8281 cgatctcgcc ctcggcgatc tccatgttga cgtcgaagag gacctggagt ccgtcgtagc
     8341 cgacgttgag gtcgcggacg agcagcagct tggagcgtcc cgcccgtcgt tcgagcagcg
     8401 cctgggaccg ggcggtggag gacttccaca cgtcctcgat gtcacgttgg atgaccgagc
     8461 cacccgagga gatcatcaga ccgccgacca gcaggatcgg cgtcatgacc agcatgccca
     8521 ggcgggttcc ccagttgtca ccgatccagc cgatgagcgg caacagggcg aggccgggga
     8581 tggcccacca cgacgccacc gagaacccga cggcgcgggc ccgggccggg atcgccagcg
     8641 acaacgtggc caggatcccc ggcaggagga tggccaggtc cgcggtcagc aggacgttga
     8701 acaccaccgc gaccgcgatg ttcggggcgt aggcgaaccc ggccgcgagc gccgcggcga
     8761 cgaagctgac ccagcgcagc agccggaaga tcagcgccgg gctcttgagg aacagccggg
     8821 tgccgagacg ggcaccgacg gccagaccga gcagctggaa cggctcgacc accgcagcca
     8881 ggtacccccg ctggagctcg tcgaggcggt acacgtcgga gtagaggagg ctggccagcg
     8941 acacgaaccc gatcagcgac acggccagga acggcaccgc gtaccagatg cggcgcagca
     9001 cctcgatctt ccacagcagc cgccacgact cggcgaagct cggtatcggc tcctcggtct
     9061 cgacgacctc gtcggaggcg cccgacgccc ggcgctcctg ggcgccacgg atcggctcct
     9121 tcatccgagt ccccaggacc accaggatca ccgtcggcac ggcgaagacc aggaacggca
     9181 cccgccagtc ggtcaggtag gcgatcccac cggccagcag cgggccgagg aactggccca
     9241 ggacgttggc cgcccggtgg aagctgaaca ccgccggccg ccggtcgacc gggaaccagt
     9301 ccgacagcag cgaggcgtgg gtggggtcga ccacggcccg tccgatgccc gcaccggcgc
     9361 gggcgacgat cagcatccag atcgcggtgg acaacccggt catgatcgag aacgccgccc
     9421 acgtcgccgc gccgagcagg gtgatgaaca cccggttgcc gcggtcggcc cacaccgcga
     9481 tcggcatctg catcaggaag gcgccgagca gggtcagcgc caccagcgtg aggatgccgg
     9541 tgttcgacat cccgaaggcg tcgcgcacgt tgggcagcag gatgccgaag ccggtgcggt
     9601 cgagctcgtc gacgcagttg aggccgaaca ggatcagcag cgcgtacacc ggtcccgagc
     9661 cggccacctc accgacggcg cggatcggat gggccagcgt ggccg
//
LOCUS       DAOQOD010000121         9381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2690_length_9381_cov_11.658267, whole genome shotgun sequence.
ACCESSION   DAOQOD010000121 DAOQOD010000000
VERSION     DAOQOD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_9381_cov_11.658267"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="PLZ72_11485"
     CDS             complement(<1..2277)
                     /locus_tag="PLZ72_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA36963.1"
                     /translation="MHSQWPISGRSTELDHLTTLLAGGGTGVLVVGEEGVGKSRVLSE
                     VTDRLRSQGMATVVVTATPGSAVLPLGSLASLLPAGSTSTGLPVLPVLRAAIETLADG
                     RRPVLVIDDVEHLDDASAVLVNQLVATGDAGLAATQTSGTIAPEPIARLWQDGMIERV
                     ELPPLTPDELASLAESIVGSPLDDRSFDLVRDMVGGNALFLREILIASRESGNLEVDD
                     GLVRIRELPVSAPRLVDFVRHRVGDLDTVASEALLLIAVGEPLGPGELPPQVSPELLA
                     RLDAAGLVTTTLDGHRVVVRLVHHLFGEVLRAAASPLQLQKAHRDLVTAVQAHGSARR
                     GDDLRLAHWSVVGQVDVDPDLLVEAARTARFSQDLELARDLAETAWRDHPDFDTGDLL
                     ADIYYELGDTGSAAAIRPRWQELAVDDDQQIRVELNAAVNGFWKEGDADAATAALDRA
                     QAHPPSVWRDEAAAVRSVFLASQGRAAEAVEIAEPLIERDPDRVMIQAAMALTHAYRT
                     QGRVEDAVAVCDRALAAYAELGEQIALISMRVLGVGRTIALAEAGRLEDADSEAARIL
                     QLCRTEGEVGGIGLAALVQGWVHFTRGRIRSARQALRLAEDSFARTGHAGMRQWAHIA
                     LALVCATSGDAPGAQGWLDAIDRDGLQPAAMFSAALARARAWTAWGAGDPESARDLLI
                     AGSESARGTGDAQGELACLHDLARLGAAEAALERRAGSEPHVQGALNAALADHISAMA
                     SGRAAELGDAAEAMADLGA"
     gene            2389..2709
                     /locus_tag="PLZ72_11490"
     CDS             2389..2709
                     /locus_tag="PLZ72_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36964.1"
                     /translation="MDHGGIAFEPSPDEVLRRGDPTRAVVVHARSSGLRDDQGRELFT
                     LVLNVREGDGSVQLTAVVALDETETDLVAEGADLPVAVLTNRGRIVAVDLTRARQERE
                     SESG"
     gene            complement(2738..3958)
                     /locus_tag="PLZ72_11495"
     CDS             complement(2738..3958)
                     /locus_tag="PLZ72_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNA36965.1"
                     /translation="MTGSITESIAGSADRPTTGRTAADRIGSSAIRDLLALTERPGVI
                     SLAGGLPDLDLLDPERIRAAADHAASVSGAHGPVALQYGPTAGLATLREVVADRLGVD
                     ADEVVITTGSQQGLDLLARSVIAPGDEVALQEPAYLGAVQAFRAAGATVVGLPGDDRG
                     LDVDALSARIAAGNAPRVVYVVADHHNPTGAVMSPGRRVRLRELADANGILVIEDAAY
                     RDLRWGPPTRPLFAPRSPVVHVGSASKVLAPGLRVGWMTGPTDVIDAVLRLKQAADLH
                     TPTWNQLMVAHLLDDGHQPRHLERIRTAYRSRAVALADELTSRLGDAVEVTPPDGGMF
                     LWMTTRDGTDTDGLLDRAIEAGVAFVPGSAFRTRPPGSDTEADHTTARLAFASRPEEQ
                     LRAAARRLAEVWRP"
     gene            4150..5109
                     /locus_tag="PLZ72_11500"
     CDS             4150..5109
                     /locus_tag="PLZ72_11500"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA36966.1"
                     /translation="MSTGSDADPDGRPRGGRDGGQDSGRDGGQDSERDGERERELGDF
                     LRSRRAAIDPADVGLPSGPRRRAPGLRREEVALLAGVSVTWYTWLEQGRAERPSRSVL
                     DAVARALRLDDAEHSHLLTLARRAAGDGAVEASSRARRAGEGVDAVDLAGHPPDALVR
                     TLDALDPAPSYLLGPRWEYLAWNRAQGLLYPKAESLAVEDRNLLFIVLCEPSARELVV
                     DREDEARAMVTEFRAATALLRGDPDLEELIARLRRESPEFECWWSGLDVAGFHSRLRR
                     YDHPRAGRLTFEYQAFEPAEWPGLRLVTQLPVPGDDSTERLSS"
     gene            5627..6946
                     /locus_tag="PLZ72_11505"
     CDS             5627..6946
                     /locus_tag="PLZ72_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNA36967.1"
                     /translation="MDPAGPPTFPGAATGGYPLGPPAAFPSGGWTPPPAPPAPASGRS
                     GGGRGTKVAIGLLLVLALLAGVAGGLGLSQLTQSGSDTAAPTRPVPDPSDHGSPFGGA
                     TTEPAPSNGSDPSGNSGSAETADADAVAGKVVPGVVNINTLTAQGQGAGTGITLREDG
                     LIVTNNHVIRGATRIVVTDADTGQRYEAKVVGTAKTKDLAVIQLQDAKGLDVVTIGDS
                     DTVRVGDAVVALGNAGGQGGTPSVVTGNVKALGRSITASDESGSRAQRLSNLIQIDAD
                     IVPGDSGGPLANADGEVIGINAAAASTNEVSTNDEGYAIPINDAMEIVEQIKAGKASD
                     VVRIGARGVLGVQVTNVADDPFGAAAGAPTGALVGGVAEGSGAAKAGVTQGSTITSVD
                     GTEVTSAEALTSALSAAKPGDRVKLTWTDQAGASHTESVTLTEGPPD"
     gene            6970..7812
                     /locus_tag="PLZ72_11510"
     CDS             6970..7812
                     /locus_tag="PLZ72_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNA36968.1"
                     /translation="MHVSEIWRYPVKSMIGEPVEAAEMVDDGMVGDRMWAARDEVRGG
                     IRGAKKIGALMRLAAAYDDGPGSAVTITLPDGTTVRAGEDGADQAVSTALDHQVTLWP
                     RMPATDLDHYRRGAPDSDDLLAELQSIFGREPDEPLPDLSVFPPEILEYESPLGTYYD
                     AFPLMIMSTSAFRSMAEAVPDSVIDVRRFRPSLIVDTGDRGGHPELDWIGSRVSVGEV
                     ELEIVTGCPRCIMVTREIDDAAPGDRAILRHIVADLDQIVGVYARILRTGTVRVSDPV
                     TVDR"
     gene            7822..8073
                     /locus_tag="PLZ72_11515"
     CDS             7822..8073
                     /locus_tag="PLZ72_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNA36969.1"
                     /translation="MLILAIIAFGMLVGWLAQLVLGMGTRPNAQSLIAGLVGSFVGGT
                     LANLIAGDGLDIAPSGLIGSFVGAVIVLLIWRLVDSRKS"
     gene            complement(8377..9183)
                     /locus_tag="PLZ72_11520"
     CDS             complement(8377..9183)
                     /locus_tag="PLZ72_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNA36970.1"
                     /translation="MIDPVDADAPNPSPGDDSPGDDNLADDSPVNAEPLHFHRLRRFV
                     RPAEQKSIAPVLGLTVLAMALSPFQADHDWSRGLEVAIIGVSAIVALERTGASRVIRR
                     AGIAIVVLATAVAATIPVLHAGRTGSLEQVAAGLFALLLLVTPVLVVIRLMLRPRITI
                     DTLAGALTAYLQIGIFFGALYLFVALVTEPSPFFAQNATPVYSDFEYFSFITMTTVGY
                     GDLTPASRIGQTLTTFEAVIGQVFLVTVVALTVSNLGRPTRRGSGQTPPP"
BASE COUNT         1270 a         3361 c         3462 g         1288 t
ORIGIN      
        1 ggcgccgagg tcggccatcg cctcggcggc gtcgcccaac tcggcggcgc ggcccgacgc
       61 catcgccgag atgtggtcgg ccagggcggc gttgagagcg ccctggacat ggggctcaga
      121 cccggcccgt cgttcgagcg ccgcctccgc tgcccccagg cgggcgaggt cgtgcagaca
      181 ggcgagctcg ccctgggcgt cgccggttcc tcgggcgctc tccgatccgg cgatgagcag
      241 gtcgcgggcc gactcggggt cgccggcgcc ccaggccgtc cacgcccggg cccgggccag
      301 ggcagcgctg aacatggccg cgggctgcag gccgtcgcgg tcgatcgcgt ccagccaccc
      361 ctgggccccc ggtgcgtcgc cggaggtggc gcacaccagg gccagtgcga tgtgggccca
      421 ctggcgcatc ccggcgtgcc cggtccgggc gaaggagtcc tcggccagac gcagggcctg
      481 gcgggccgat cggatgcgac cgcgggtgaa gtggacccag ccctgcacga gcgccgccag
      541 accgatgccg ccgacctcgc cctcggtccg gcacagctgg aggatcctcg ccgcctccga
      601 gtcggcgtcc tccaggcgac cggcctcggc cagggcgatg gtgcggccga cccccagcac
      661 gcgcatcgag atgagggcga tctgctcgcc cagctccgcg tatgcggcca gcgcccggtc
      721 gcagaccgcc acggcgtcct ccacccggcc ctgggtccgg taggcgtggg tcagcgccat
      781 cgccgcctgg atcatgacgc ggtccgggtc gcgctcgatc agcggctcgg cgatctcgac
      841 ggcctccgcg gctcgaccct gcgacgccag gaacaccgat cggaccgccg cggcctcgtc
      901 gcgccacacc gacggggggt gggcctgggc gcggtccagg gccgcggtgg cggcatcggc
      961 gtcgccctcc ttccagaacc cgttgaccgc ggcgttgagc tcgacgcgga tctgctggtc
     1021 gtcgtcgacg gccagctcct gccatcgcgg ccggatggcg gcggccgacc cggtgtcgcc
     1081 gagctcgtag tagatgtcgg cgagcaggtc tccggtgtcg aagtcggggt ggtcccgcca
     1141 cgcggtctcg gccaggtcgc gtgcgagctc caggtcctgg ctgaagcggg cggtccgcgc
     1201 cgcctcgacg agcaggtcgg ggtcgacgtc gacctgtccg accaccgacc agtgcgccag
     1261 gcggaggtcg tcgccgcggc gggcggagcc gtgggcctgc accgcggtga ccaggtcccg
     1321 gtgggccttc tggagctgca ggggcgacgc cgcagcgcgg agcacctcgc cgaagaggtg
     1381 gtgcacgagt cgcaccacga cacggtggcc gtcgagcgtg gtcgtgacca ggccggcggc
     1441 gtccaggcgg gccaggagtt cggggctgac ctgcggaggg agctcgccgg ggccgagggg
     1501 ttctccgacc gcgatcagca gcagcgcctc cgaggcgacc gtgtcgaggt cgccgacacg
     1561 gtggcggacg aagtcgacga ggcgtggggc cgacaccggg agctcccgga tgcggaccag
     1621 gccgtcgtcg acctcgaggt tccccgattc gcgggaggcg atcaggatct cgcggaggaa
     1681 cagagcgttc ccgccgacca tgtcgcggac caggtcgaag gaccggtcgt cgagcgggga
     1741 gccgacgatc gactcggcca ggctggcgag ctcgtcgggg gtcagcggcg gcagctcgac
     1801 ccgttcgatc atcccgtcct gccagagccg cgcgatcggc tcgggggcga tggtgcccga
     1861 cgtctgggtc gcggccaggc cggcgtcacc ggtggcgacg agctggttga ccagcacggc
     1921 cgatgcgtcg tcgaggtgct cgacgtcgtc gatgaccagc accggtcgac gcccgtcggc
     1981 cagcgtctcg atggcggcgc gcagcaccgg gagcaccggg agtccggtcg aggtggagcc
     2041 ggccggcagg agcgacgcca ggctgccgag tggcagcacg gccgatcccg gggtggccgt
     2101 cacgacgacg gtcgccatcc cctgggagcg gagccggtcg gtgacctcgc tgagaacccg
     2161 gctctttccg acaccttcct ccccgacgac gagcacgccg gtcccgccgc cggccagcag
     2221 cgtggtgagg tgatcgagct cggtggagcg gccgctgatc ggccactggc tgtgcatggt
     2281 cctccgcgtt ggggtcgacc gaggtgggcg ccatcgtgcg tgcggtcgtg acctcggtca
     2341 gttgctgagt attgcgtatg ggtcaccgat gcgccccgca cgggaggggt ggaccatggg
     2401 gggatcgctt tcgaaccctc acccgacgag gtcctccggc gtggagatcc gacccgggcg
     2461 gtcgtggtgc acgcccgcag ctccggactt cgcgacgacc agggccggga gctgttcacg
     2521 ctggttctca acgtgcgcga gggcgacggg tcggtccagt tgaccgccgt ggtcgccctc
     2581 gacgagaccg agaccgacct ggtggcggag ggtgctgacc tgccggtcgc agtcctgacg
     2641 aaccggggtc ggatcgtcgc ggtcgacctg acccgggccc gccaggagcg cgagtcggag
     2701 tcgggctgag cctcgagccc gagaacgtcc tgatcgctca gggtcgccac acctcggcca
     2761 gtcgtcgtgc cgccgcacgg agctgctctt cgggtcggga cgcgaacgcc agccgagcgg
     2821 tggtgtggtc ggcctcggtg tcggaccctg gcggccgggt ccggaacgcc gagccgggga
     2881 cgaaggcgac gccggcctcg atcgcacggt ccaggaggcc gtcggtgtcc gtcccgtccc
     2941 gggtggtcat ccacaggaac atgccgccgt cgggcggggt cacctcgacg gcgtcgccga
     3001 gccgggaggt cagctcgtcg gccagggcca cggcccggga gcggtacgcg gtgcggatgc
     3061 gctccaggtg tcgcggctgg tggccgtcgt cgagcaggtg ggcgaccatc agctggttcc
     3121 atgtgggggt gtgcaggtcg gcggcctgct tgaggcggag gaccgcgtcg atcacgtcgg
     3181 tcggtcccgt catccagccg accctcagcc cgggggcgag caccttggac gcggatccga
     3241 cgtgcacgac cggactccgg ggagcgaaca gcggacgggt gggcggtccc caccggaggt
     3301 cccggtaggc ggcgtcctcg atcaccagga tcccgttcgc gtcggccagc tcacggaggc
     3361 ggacccggcg gcccgggctc atcacggccc cggtcgggtt gtggtggtcg gccaccacgt
     3421 agacgacccg cggtgcgttc cccgcagcga tgcgggcgct gagcgcgtcg acgtcgagtc
     3481 cacggtcgtc gcccgggagc ccgacgaccg tggcgcccgc ggcccggaag gcctggacgg
     3541 cgcccaggta ggccggctcc tgcagcgcga cctcgtcgcc gggggcgatc acggagcggg
     3601 ccaacaggtc gaggccctgt tgggatcccg tggtgatcac gacctcgtcg gcgtcgacgc
     3661 ccagtcgatc ggccaccact tcccgcagcg tcgccagacc ggcggtcggc ccgtactgca
     3721 gcgcgaccgg gccgtgggca ccgctgaccg aagccgcatg gtcggccgcg gcacggatcc
     3781 gctccgggtc gagcaggtcg aggtcgggca ggccgccggc gagggagatg acgccgggtc
     3841 gttcggtcag cgcgagcagg tcccggatgg ccgacgatcc gatccggtcc gccgcggttc
     3901 gcccggtcgt cggccggtca gccgatccgg cgatcgattc ggtgatcgat ccggtcactg
     3961 acccggtggt ctgccgcgcc gggcggcagc tggtgccgcc ccgatcgggg ggcggggcga
     4021 ctgcggacag gccgtggggc gaggtcatcg gtcgatcgga ccacgacggt ccgtccgggt
     4081 gggagtgtgc gctgatgctg gtgtcggcac cacctgcctc ggcccgtccc gatctgctcc
     4141 actggggaga tgtccaccgg ttccgacgcc gatcccgacg gccgaccgcg gggtgggcgg
     4201 gacggcggtc aggacagcgg tcgggacggt ggtcaggaca gcgagcggga tggcgagcgg
     4261 gagcgcgagc tcggcgactt cctgcgctcc cgccgggcag cgatcgaccc ggccgacgtc
     4321 gggctcccga gcggaccccg tcgccgcgcc cccgggttgc gtcgcgagga ggtcgccctg
     4381 ctcgccggcg tgtcggtcac ctggtacacg tggctggagc agggccgggc cgaacggccg
     4441 tcgcggtcgg tgctcgacgc tgtggcccgg gcgttgcgtc tggacgacgc cgagcactcg
     4501 cacctgctga ccctggcccg gcgcgccgcg ggcgacggcg ccgtcgaggc ctcgagtcgg
     4561 gcccgtcgag caggggaggg cgtcgatgcg gtcgacctcg ccggtcaccc tccggacgcc
     4621 ctggtgcgaa ccctcgacgc gctcgatcct gcgccgagct acctgctcgg tccccgctgg
     4681 gagtacctgg cgtggaaccg ggcacaggga ctgctctacc ccaaggccga gtcgctggcg
     4741 gtcgaggacc ggaacctgct gttcatcgtg ctgtgcgagc cgtccgcccg ggagctggtg
     4801 gtcgaccgtg aggatgaggc gcgggcgatg gtcaccgagt tccgcgccgc caccgcgctg
     4861 ctccgcggcg accccgacct ggaggagctg atcgcccgac tgcgtcggga gtcgccggag
     4921 ttcgaatgct ggtggtccgg tctcgacgtg gccgggttcc acagccggct gcgccgctac
     4981 gaccaccccc gagcgggtcg gctgaccttc gagtaccagg cgttcgagcc ggcggagtgg
     5041 cccggcctcc gactggtgac ccagctaccg gtccccggcg acgacagcac cgagcggctg
     5101 tcgagctgac gcatacccat acccccaccc cccaccccca ccctcgcttt gtcccgcaca
     5161 gacgtcgtga tcacgacgtc tgtgcgggac aaagctggtt cgggcgggac agagcgggtt
     5221 ctggtgggtg gccgatcggc ccggaccttg ccatgctggg agcccgggtc cgctccggcg
     5281 atgcacacgc ggcgctgagg cggaccacag tccctcaggg tcctcacagg tcccgcttac
     5341 cctgccctca cgttccggga gtgacatggc ggccatgtcc cacggcgacc ccacgtccga
     5401 acccacgccc ccggccggag caccggccgg gccgccccga ccgctcggcc cccttcccgc
     5461 cgcggtcact gctccgaccg cggttgtcgg tccgctcggg ccgcccccgg cgcccacagc
     5521 ggggttgccc ccgctcgggt cgtcggcccc cggcggtgcg cctgctggtc cgcctcaggg
     5581 tcctcccacc gggccaccgt cggttccgac cctcggtccg ccgaccgtgg acccggccgg
     5641 tccgccgacc ttcccgggcg cggcgacggg tggctacccg ctcggtccgc ccgcggcctt
     5701 cccctcgggc ggctggacgc ctcccccggc gcccccggct cccgcatcgg gccgctccgg
     5761 cggcgggcgc ggaaccaagg tcgccatcgg cctcctgttg gtcctcgccc tgctcgccgg
     5821 ggtcgccggc ggactcgggt tgtcgcagct cacgcagtcg ggctccgaca ccgctgcacc
     5881 gacacgtccc gtcccggacc cgtccgacca cggctccccg ttcggtggcg ccacgaccga
     5941 acctgcgccg tcgaacgggt cggacccgtc gggcaactcg ggctcggccg agaccgccga
     6001 cgccgacgcc gtagccggca aggtcgtccc gggcgtggtg aacatcaaca cgctcaccgc
     6061 ccagggccag ggcgccggga ccggcatcac cctgcgggaa gacggcctga tcgtcaccaa
     6121 caaccacgtc atccgcggcg ccacccgcat cgtcgtcacc gacgccgaca ccgggcagcg
     6181 ctacgaggcc aaggtggtcg gaaccgccaa gaccaaggac ctggcagtga tccagctgca
     6241 ggacgccaag ggtctggacg tggtcacgat cggtgactcc gacacggtgc gcgtcggcga
     6301 cgccgtggtc gctctcggca acgccggtgg ccagggcggc acgccgtcgg tcgtgaccgg
     6361 caacgtcaag gcgctcggtc gcagcatcac cgccagcgac gagtcgggca gccgggccca
     6421 gcgcctgagc aacctcatcc agatcgacgc cgacatcgtc cccggcgact ccggcggacc
     6481 gctcgccaac gccgacggcg aggtgatcgg catcaacgcc gccgccgcgt cgaccaacga
     6541 ggtgtcgacc aacgacgagg gctacgccat cccgatcaac gacgccatgg agatcgtcga
     6601 gcagatcaag gccggcaagg ccagcgacgt cgtccgcatc ggcgcccggg gcgtcctcgg
     6661 cgtgcaggtg accaacgtgg ccgacgatcc gttcggcgcc gcggccgggg cgcccaccgg
     6721 ggcgttggtc ggcggtgtgg ccgaaggctc cggtgccgcc aaggcaggcg tgactcaggg
     6781 ctccacgatc acctcggtcg acggcaccga ggtcacttcg gccgaggcgt tgacctcggc
     6841 gctctctgcg gccaagcccg gagaccgggt caagctgacc tggaccgatc aggccggggc
     6901 atcgcacacc gagtcggtga ccctcaccga gggcccgccc gactgacctc gagcggtagc
     6961 gtgccgacgg tgcacgtgag cgagatctgg cggtacccgg tcaagtcgat gatcggtgag
     7021 cccgtcgagg ccgccgaaat ggtcgacgac ggcatggtcg gcgaccggat gtgggcggct
     7081 cgggacgagg tccggggcgg catccggggc gccaagaaga tcggcgccct gatgaggctc
     7141 gccgcggcgt acgacgacgg cccgggctcg gcggtcacca tcaccctgcc cgatgggacc
     7201 acggtccgcg ccggcgagga cggtgccgac caggcggtga gcacggcgct cgatcaccag
     7261 gtcacgctgt ggccgcggat gccggccacc gatctcgacc actaccgccg aggcgcgccg
     7321 gactccgacg acctgctcgc ggagttgcag tccatcttcg gccgggaacc cgacgagccg
     7381 ctccctgacc tgtcggtctt cccgcccgag atcctcgagt acgagtcgcc gctcggcacg
     7441 tactacgacg ccttcccgtt gatgatcatg tccacctcgg ccttccggtc catggccgag
     7501 gccgtccccg actcggtgat cgacgtccgt cggttccgtc ccagcctgat cgtcgacacc
     7561 ggtgaccgag gggggcaccc cgagctcgac tggatcggat cgcgcgtgtc ggtgggtgag
     7621 gtggagctgg agatcgtgac cgggtgtccg cgctgcatca tggtcacccg cgagatcgac
     7681 gacgccgcgc cgggagaccg ggcgatcctg cgtcacatcg tggctgacct cgaccagatc
     7741 gtcggcgtgt acgcccggat cctgcggacc ggcacggtcc gcgtctcgga cccggtcacg
     7801 gtggaccgct agggtccgcc cgtgctgatc ctcgccatca tcgctttcgg aatgctcgtc
     7861 ggctggctgg cccagctcgt cctcggcatg gggacccgac ccaacgccca gtcgctgatc
     7921 gccggactgg tcggttcgtt cgtcggcgga acgctggcca acctgatcgc aggcgatggc
     7981 ctcgacatcg caccgagcgg tctgatcggg tccttcgtgg gcgccgtgat cgtgctgctg
     8041 atctggcgcc tggtcgactc ccgcaagtcc tgagcaccct cccccttccg cttcgtcccg
     8101 cacaggcgcg gtgatcaccg cgcctgtgcg ggacaatgcg ggttcaaccg acgaggccgg
     8161 cgccttcctc tcgccgaccc cgtcgatgtc ctcccatcga cggacccacc cacgccgacc
     8221 ggcgttcgcc gctcggtcgt cgcggacgtg atccgtcccc tcctcaccgt cagcatcggt
     8281 cgacggcggc cgctcgacaa tggggacgag tccccggccc atcgctctgt cctgcacagg
     8341 cgcggtgatc accgcgcctg tgcaggacaa agcgggtcag gggggtgggg tctggccgga
     8401 gccgcgccgg gtgggtcgac ccaggttcga cacggtgagc gccaccaccg tcaccaggaa
     8461 gacctggccg atcacggcct cgaaggtggt cagcgtctgg ccgatccgag acgcgggggt
     8521 caggtcgccg tagccgacgg tggtcatcgt gatgaagctg aagtactcga agtccgagta
     8581 caccggggtc gcgttctggg cgaagaacgg cgagggctcg gtcacgagcg ccacgaacag
     8641 gtacagcgcc ccgaagaaga tgccgatctg gaggtaggcg gtgagcgcgc cggcgagggt
     8701 gtcgatcgtg atgcggggac gcagcatcag ccggatcacc accaggaccg gggtgacgag
     8761 cagcagcagg gcgaagaggc cggccgccac ctgctcgagc gatccggtgc gaccggcgtg
     8821 cagcaccggg atggtcgccg ccaccgccgt ggcgagcacg acgatggcga tcccggctcg
     8881 tcggatcacc cggctcgccc cggtgcgttc gagggcgacg atcgcgctca cgccgatgat
     8941 cgcgacctcg aggccgcggc tccagtcgtg gtcggcctgg aacgggctga gagccatggc
     9001 gagcacggtg agcccgagca cgggggcgat gctcttctgc tcggccgggc gcacgaaccg
     9061 gcggaggcgg tggaagtgca gcggctcggc gttgacaggg ctgtcgtcgg ccaggttgtc
     9121 gtcgcccggg ctgtcgtcgc ccgggctggg gttcggggca tctgcgtcca cggggtcgat
     9181 catgaccgca cctcgggacc cgacggtgga tcgcccgcga tcagacgggg gcggcggtcc
     9241 gacggtcccg ttcggcccga ccagcgccga agtgcgtgga caggtcgaga aggaacgcga
     9301 acaccacgat gaaccacccg aacccggaca cgctctcgat cgggttcgcc catgcgacgg
     9361 cccacgccag ggtcgtccac g
//
LOCUS       DAOQOD010000122         9285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2733_length_9285_cov_12.856230, whole genome shotgun sequence.
ACCESSION   DAOQOD010000122 DAOQOD010000000
VERSION     DAOQOD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_9285_cov_12.856230"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLZ72_11525"
     CDS             complement(<1..623)
                     /locus_tag="PLZ72_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA36971.1"
                     /translation="MPADVSPLVIALAGLGVGVVVGLTGMGGGALLTPILVLGFGIDP
                     TRAVGSDLLVSLVMKPVGGGVHLRRGTVNLPIVGWLSAGSVPSAFLAVWVMSSVFGDD
                     LDDVIQYAVGAALLLASLGLVAKGAFASRVGGSEDDLAHTAVRPLPTLLVGVFGGMAV
                     GITSVGSGSLMVPLLLMLYPLLTSKQLVGTDLVPFRCRPATLGHAAFG"
     gene            735..1628
                     /gene="panC"
                     /locus_tag="PLZ72_11530"
     CDS             735..1628
                     /gene="panC"
                     /locus_tag="PLZ72_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNA36972.1"
                     /translation="MIATVVRGVELRDRLDSERSRGHRIGFVPTMGSLHRGHAELIRT
                     AAAHTDVVVVSVFVNPLQFGAGEDLDAYPRDPAGDRDVAEAAGAAVMFAPTEVEMYPH
                     GREAVLTTVAVPRLAAVMEGASRPTHFAGVCTVVAKLWNLVGPCRAYFGEKDFQQLAI
                     LRRMAADLSFPVDVVGCPTVREPDGLALSSRNAYLSAEERAVAPVLHRALSLGAEAIL
                     AGTSDAGTVRRLMAETIDAAPLGRLDYVEVAHGDTLQPLTVATEDARLFGAVRFGRAR
                     LIDNVGVTPSSTDTDPGPRRP"
     gene            1625..3262
                     /gene="nadB"
                     /locus_tag="PLZ72_11535"
     CDS             1625..3262
                     /gene="nadB"
                     /locus_tag="PLZ72_11535"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNA36973.1"
                     /translation="MTAPATGELDLLVLGSGVAGLSAAVRASNEHGLDTGVLTKGLLE
                     QSTTRWAQGGVAAALSGDAEEVDLHLADTLAAGAGLCDLDAVRVLVDEGPRRVEELIG
                     LGAEFDRDERGDLDLAREGGHSVARVVHAGAATGAEVERALVDAVRSTAAVLLEHTFA
                     HDLLVEGGRCVGVTAVGPSGPTEVRARHVLLATGGAGQLFEVTTNPPVATGDGVAMAL
                     RSGVAVADLEFFQFHPTALALPAMPRALLSEALRGHGAVLRDRDGERFVDELLPRDVV
                     SRAILARMHEQGTDHVWLDATGLDHFAVRFPNIAASLAAVGLDASVDLLPVAPAAHHQ
                     CGGIVTDLRGATSLPGLWAAGETTCTGVHGANRLASNSLLEGMVFGPRAVEAIAAGAD
                     GPTPTGAMRCVLGFPDGEPDDDSLDIVGLPLEIPAPPDRRGGSDRGDVRGGVEEGLSA
                     VALRAAVQHAMSSGAGVVRSGDGLERCASLLDHASMLLPERPGDTATAEVLNLVEVGR
                     VLVEAARARTESRGTHARVDHPEPDPAQRHRLVVGGT"
     gene            3306..>4144
                     /gene="nadC"
                     /locus_tag="PLZ72_11540"
     CDS             3306..>4144
                     /gene="nadC"
                     /locus_tag="PLZ72_11540"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNA36974.1"
                     /translation="MSSQVDPGYLQPPTLDVVDAVRRALDEDLTPLGDLTATLVPPVL
                     GEARFVARTDGVLAGTACATETFAQLDAAVEVDWVRDDGARLSAGEVFGVVRGPFRSI
                     LTGERTALNFLGFLSGIATLTAAYVDAAAAGGDARVWDTRKTIPGLRSLSKAAVRAGG
                     GRNHRGNLSDWMLIKDNHLGGVGIAEAVAVARDRWPGRTVHVECDSLDQCVEALDAGA
                     DSILLDNMSPAEVRACVAAAQVRQGGAVRRTLLEVSGDVTLDTIGEYAATGVDMISVG
                     RIT"
     assembly_gap    4145..4244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4460..5236
                     /locus_tag="PLZ72_11545"
     CDS             4460..5236
                     /locus_tag="PLZ72_11545"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNA36975.1"
                     /translation="MLLVIDAGNTQTVVGLYDSGDRSHELLDHWRLATNAERTSDELW
                     LLLRQLLETRGLDLRTDVLGVAVSSGVPSITFELRRMCERYLDIEPVVLGTGIKTGMP
                     VLYENPREVGADRIANAVGAYDLFGGPTIVVDFGTATTFDVISDKGEYMGGAIVPGIE
                     ISMDALFGRAAALRRVELVAPRSVIGRTTVESIQSGAVFGYSSLVDGMCERIEDVIGD
                     STIVATGGLAGLIAPATTSIEHEEPWLTLHGLRLIWEKNQ"
     gene            5233..6711
                     /gene="lysS"
                     /locus_tag="PLZ72_11550"
     CDS             5233..6711
                     /gene="lysS"
                     /locus_tag="PLZ72_11550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNA36976.1"
                     /translation="MTDHRLAHWDQPYTFAPDATAASIVSDHPDLEPGTETGERVTIA
                     GRLMLRRVQGKLAFGTLADRTGRIQLFAPAATTPDFERFGELHLGDWIGVTGEVMTTR
                     RGELSVRVDEWVLLAPARRSFPDKWHGISDTDTRYRQRYVDLWVTEESRRTFEMRSRI
                     LSETRRFLEARGYLEVETPVFHPVPGGALAEPFTTHHNALDLELYLRIAPELYLKRLV
                     VGGFEKVFEIARVFRNEGISNRHNPEFTMLEAYEAYGDYHTHMALTEELVAHLALELT
                     GSTTVTFDDRPVDLSAPWRRAPLAELTSERVGEDVSVHTSPDRLRELCDRHGVPHDRT
                     YGPGKLLLELYEKTTEATLWDPTFVVDYPAEVSPLSRRHRDDPELTERFEAIVVGREL
                     CNGFSELTDPDDQRSRFEDQAARKAAGDAEAMAVDADYLRALDYGLPPTVGLGIGMDR
                     LVMLLSGTSSIRDVVLFPTLRPEPPERERPDSPSGESVESGS"
     gene            6716..7405
                     /locus_tag="PLZ72_11555"
     CDS             6716..7405
                     /locus_tag="PLZ72_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36977.1"
                     /translation="MGSLFLDFLGEEIQVEPGETLTFGRAADLIVDADNQYLHRVLGC
                     FAAQGDVWFLQNLGRHITLRVIDRDGSSRTELAPSDQAPIGFEEFVVAFDAGPASYEI
                     GGALAEPTPLELGVSVASDTVEFATINLNTEQRMLVAALAEPLLRGRTDWQSEIPSNK
                     EIARRLGWTVSKFNRKLDYLCRRLAEQGVDGMQGESGVLATSRRARLVRHMVDRRLVV
                     VADLDALPAPR"
     gene            7448..9271
                     /locus_tag="PLZ72_11560"
     CDS             7448..9271
                     /locus_tag="PLZ72_11560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA36978.1"
                     /translation="MAVGGDDVEVPGVVLDRVIGRGASSVVYLGTQTRFDRPVAVKVL
                     HLPGQTELVGKLFLNECRTLGRLSPHPNVVSVFDGGFIDDERPFLVMEYLPGGTLADE
                     LVAGGPIPFAEVLRVGVQLAGGVHSVHLHGIIHGDIKPANVLRTRTGDVALADFGIAR
                     LMSSTATTRAPLLTPLHAAPELFEGGAITPRTDVYELCSTLFELLDGRASLGEPTDSP
                     LLVLGRMARGDRRRLDRGRVPDVVATVVEAGLSTDPDQRPATALELGEQLRSAQRTLG
                     LDPTPLVVIEPLDSDESVQDPRPAIDHGPSARSSAAGTAPSRTAGVEAGRAGDTLPPP
                     DRRRSLRPLALAVAVVVAAVLVGVVVFALVRGGDDGTEVTTSGSTSTTWPEEVRGTPS
                     AGFQPGIEFNVPGASDRNAEVNARLGDGSQVLGTFGSGVVVSDQPPPFAAQLPVRFRW
                     QAFNPQLDEDCLAIVSWPLTVTGWWEKRATWSGHQAVVRVLKFASERDAVEAFNVFSL
                     EQGVTGDDCHGFRSDGKPFDHDRIEVAHRAVDLGLGDDVRWNSFLAGAPSQPAGFTSS
                     TNAIAQKGDVVVLGNVANADTPVDPATFGATFSSILTALSA"
BASE COUNT         1285 a         3375 c         3215 g         1310 t
ORIGIN      
        1 ccgaacgccg cgtggccgag cgtcgccggg cgacagcgga acgggaccag gtcggtgccg
       61 acgagctgct tggaggtcaa cagcgggtag agcatcagca gcagcggaac catgagcgag
      121 ccggatccga ccgacgtgat gccgacggcc atcccaccga agacgccgac caggagcgtg
      181 ggcagcggac ggaccgccgt gtgcgccaga tcgtcctcgg acccaccgac ccgggaggcg
      241 aacgccccct tggcgacgag accgagcgac gccaggagca gggcggcgcc cacggcgtac
      301 tggatcacgt cgtccagatc gtcaccgaag acgctgctca tcacccacac cgccaggaac
      361 gccgagggga ccgagccggc cgacagccac cccacgatcg gcaggttcac cgttcctcga
      421 cgcaggtgga ccccgccccc caccggcttc atgaccagcg ataccagcag gtcgctgccg
      481 acggcccggg tcgggtcgat cccgaagccc aggaccagga tcggggtcag cagtgcgccg
      541 ccgcccatcc cggtcagccc gacgaccacg ccgacgccga ggccggccag ggcgatgacc
      601 agcggggaga catcggcggg catgcggctc cgaagggacg aggtgggccc ggcaggaccc
      661 gaccggggcc tgggcattca actaatagtt gagcggataa gtcaactatt gccgggaggg
      721 atagcgtgcc gcccgtgatc gccaccgtcg tgaggggcgt cgagctgcgg gaccgactcg
      781 actccgagcg gtcgcggggt caccggatcg gcttcgtccc gaccatgggc tcgttgcacc
      841 gaggtcatgc cgagctgatc cggaccgcgg ccgcgcacac cgacgtggtc gtcgtgtcgg
      901 tgttcgtcaa cccgctgcag ttcggcgcgg gtgaggatct ggacgcgtac ccccgcgacc
      961 cggccggcga ccgggacgtg gcggaggcgg caggagccgc ggtgatgttc gcccccaccg
     1021 aggtcgagat gtatccgcac ggccgggagg ccgtgctgac cacggtggcc gtcccgcgcc
     1081 tcgccgcggt gatggagggg gcgtcacgtc cgacccactt cgcgggtgtg tgcacggtgg
     1141 tcgccaagct ctggaacctc gtcggtccgt gccgcgccta cttcggcgag aaggacttcc
     1201 agcagctcgc gatcctgcgg cgaatggccg ccgacctgtc gttcccggtc gacgtcgtcg
     1261 gctgcccgac cgtccgggaa cccgacggac tggccctgtc gagccgcaac gcctacctga
     1321 gcgccgagga gcgtgcggtc gctccggtcc tgcaccgggc cctgagcctc ggggccgagg
     1381 cgatcctcgc ggggacctcc gacgccggga cggttcgtcg gctcatggcc gagacgatcg
     1441 acgccgcgcc cctcggtcga ctcgactacg tcgaggtcgc ccacggcgac accctccagc
     1501 cactcaccgt cgcgaccgag gacgcccgac tgttcggcgc tgtgcgcttc ggccgcgcac
     1561 gactgatcga caacgtcggc gtcactccct cgagcaccga caccgatccc ggaccccggc
     1621 gaccgtgacc gctcccgcga ccggcgagct ggacctcctc gtcctcggca gcggtgtcgc
     1681 cgggctgtcg gcggccgtcc gggcgagcaa cgagcacggc ctggacaccg gggtactgac
     1741 caagggcctg ctggagcagt cgacgacccg ctgggcccag ggcggggtcg ccgccgcgtt
     1801 gagcggcgac gccgaagagg tcgacctgca cctggccgac acgctggccg ccggagccgg
     1861 gctctgcgac ctcgacgccg tgcgcgtgct ggtcgacgag gggccgcgtc gggtcgagga
     1921 gctgatcggc ctcggagcgg agttcgaccg cgacgaacga ggagacctgg acctggcccg
     1981 cgaaggcggc cactccgtcg cccgggtcgt ccacgccggt gccgccaccg gcgccgaggt
     2041 cgagcgagcg ctcgtcgacg ccgtgcggag caccgccgcg gtcctgctcg agcacacctt
     2101 cgcccacgac ctgctcgtcg agggcggccg gtgcgtcggc gtcaccgcgg tcggtccgtc
     2161 gggtccgacc gaggtccggg cccggcacgt actgctcgcc accggcggcg ccggccagct
     2221 gttcgaggtc accaccaacc caccggtggc gaccggcgac ggggtggcga tggcccttcg
     2281 gtccggcgtg gcggtggccg acctcgagtt cttccagttc caccccaccg ccctcgctct
     2341 accggccatg ccccgagcgc tgctgtcaga ggcgttgcga ggacacggcg ccgtcctgcg
     2401 cgaccgtgac ggcgaacggt tcgtcgacga gctcctgccg cgggacgtcg tgtcgcgggc
     2461 gatcctggcc aggatgcacg agcagggcac cgaccacgtg tggctcgatg ccacggggct
     2521 cgaccacttc gccgtccgct tccccaacat cgccgcctcg ctggccgcgg tcggcctcga
     2581 cgcctcggtc gacctcctgc cggtggcgcc ggccgcccac caccagtgcg gcgggatcgt
     2641 caccgacctg aggggggcca cgtcgctgcc gggactgtgg gcggcgggag agaccacgtg
     2701 caccggcgtg cacggcgcca accgcctggc gtcgaactcg ctgctcgagg gcatggtgtt
     2761 cggcccgcgg gccgtcgagg ccatcgccgc gggagcggac ggaccgaccc cgacgggtgc
     2821 catgcgctgc gtcctcggct tccccgacgg ggagcccgac gacgactcgc tcgacatcgt
     2881 cggactgccg ctcgagatcc ccgccccgcc cgatcgccgg ggcggatcgg accgcggcga
     2941 cgttcggggc ggcgtcgagg agggactctc cgccgtcgcg ttgcgggccg cggtccagca
     3001 cgccatgtcc agcggggcgg gagtcgtgcg aagtggtgac gggctcgagc ggtgcgcgtc
     3061 cctgttggac cacgcgtcga tgctgctgcc ggaacggccg ggcgacacgg cgaccgccga
     3121 ggtgctgaac ctggtcgagg tcggacgggt gctcgtagag gcggcacggg cacgaaccga
     3181 gtcgcggggc acccacgccc gcgtcgacca ccccgagccc gatcccgccc agcgccatcg
     3241 gctggtcgtc ggcgggacct gagcccaccg tgacggggac cccccggtcg gggcaggctc
     3301 tcgccatgag ctcccaggtc gatcccggct acctgcaacc acccaccctc gacgtggtcg
     3361 atgccgtgcg gcgggcgctg gacgaggacc tcaccccgct cggcgacctg accgcgaccc
     3421 tggtgccgcc ggtgttggga gaggcccgct tcgtcgcccg caccgacggc gtgctcgccg
     3481 ggaccgcgtg cgccacggag accttcgccc agctcgacgc agccgtcgag gtcgactggg
     3541 tgcgcgacga cggggcccgg ctgtcggccg gcgaggtgtt cggcgtcgtg cgaggaccgt
     3601 tccggtcgat cctcaccggc gagcgcaccg ccttgaactt cctgggcttc ctgtcgggga
     3661 tcgcgacgct caccgccgcc tacgtcgacg cggccgcggc cggcggcgac gcccgggtct
     3721 gggatacccg caagaccatc ccggggctgc ggtcgctgtc caaggcggcg gtgcgcgccg
     3781 gcggtggtcg caaccaccgc gggaacctgt cggactggat gctgatcaag gacaaccacc
     3841 tgggtggcgt gggcatcgcc gaggcggtgg ccgtggcccg ggaccgctgg cccggccgca
     3901 cggtgcacgt cgagtgcgac tcgctggatc agtgcgtcga ggccctcgat gccggagcgg
     3961 actcgatcct gctggacaac atgagcccgg ccgaggtccg ggcctgcgtc gccgcggccc
     4021 aggtccgcca gggcggcgcg gtgcgccgca ccctgctcga ggtgtccggt gacgtcaccc
     4081 tcgacacgat cggcgagtac gccgcgaccg gggtggacat gatctcggtc ggtcggatca
     4141 ccaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccggc gttgttgtag
     4261 ccctcggtcg ctggcgtgac cgctcagcta caagttcggc ggtggctccg gacggggaac
     4321 gggtgccggc ggaccgcggt gcgtctggca tcctgtgacg gatcgccgtc cactgggccc
     4381 cggctcggaa cccgacggcc ccacacggca gacaactccc gaagccagac cctccgcgaa
     4441 gacagacagg accccgaacg tgctgctcgt catcgacgcc ggcaacaccc agaccgtcgt
     4501 cggcctctac gactccggtg accgcagcca cgagctgctc gaccactggc gtctggccac
     4561 caacgcggag cggaccagcg acgagctgtg gctcctgctg cgccagctcc tcgagacccg
     4621 tggcctcgac ctgcgcaccg acgtcctggg tgtggcggtc tcctcgggcg tgcccagcat
     4681 caccttcgag ctgcgtcgga tgtgcgagcg gtacctcgac atcgagccgg tcgtcctcgg
     4741 aaccggcatc aagacgggga tgccggttct gtacgagaac ccccgcgagg tcggtgcgga
     4801 ccggatcgcc aacgcggtgg gggcctacga cctgttcggc ggtccgacga tcgtcgtcga
     4861 cttcggcacc gcgacgacct tcgacgtcat ctccgacaag ggcgagtaca tgggcggcgc
     4921 catcgtgccg gggatcgaga tctccatgga cgccctgttc gggagggccg ccgcactgcg
     4981 tcgggtggag ctggtcgcac cccgcagcgt gatcggtcgg accaccgtcg agtcgatcca
     5041 gtccggagcg gtcttcggct actcgtcgct cgtcgacggt atgtgcgagc gcatcgagga
     5101 cgtcatcggc gactccacga tcgtcgccac cggcgggctg gcgggcctga tcgctcccgc
     5161 caccacctcg atcgagcacg aagagccctg gctcacgctg cacggcctcc gcctcatctg
     5221 ggagaagaac cagtgaccga ccaccgtctg gcccactggg accagccgta cacgttcgct
     5281 cccgacgcca ccgcagcgtc gatcgtgtcg gatcaccccg acctcgagcc gggcaccgag
     5341 accggcgagc gggtgaccat cgccgggcgg ctcatgctgc gccgcgtgca gggcaagttg
     5401 gccttcggca cgctggcgga ccgcaccggt cggatccagc tgttcgcccc cgcggcgacg
     5461 accccggact tcgagcgctt cggcgagctg cacctcggag attggatcgg ggtcaccgga
     5521 gaggtcatga ccacccgtcg cggtgagctg tcggtccgcg tcgacgagtg ggtgctgctc
     5581 gctcccgccc gacgctcgtt ccccgacaag tggcacggca tcagcgacac cgacacccgc
     5641 taccggcagc gctacgtcga cctgtgggtc accgaggagt cccggcgcac gttcgagatg
     5701 cgctcccgga tcctgtccga gacgcgccgc ttcctcgagg cccgcgggta cctggaggtg
     5761 gagacgccgg tgttccaccc ggtccccggc ggagccctgg ccgagccgtt caccacccac
     5821 cacaacgcgc tcgacctgga gctgtacctg cgcatcgctc cggagctgta cctgaagcgc
     5881 ctggtggtcg gcggcttcga gaaggtgttc gagatcgccc gggtgttccg caacgagggc
     5941 atctccaacc gtcacaaccc cgagttcacg atgctcgagg cctacgaggc ctacggcgac
     6001 taccacaccc acatggcgct caccgaagag ctggtcgcgc acctggcgct cgagctgacc
     6061 ggatcgacca ccgtcacctt cgacgaccgc ccggtcgacc tgtcggctcc gtggcgtcgc
     6121 gctccgctgg ccgagctcac ctccgagcgg gtgggcgagg acgtgtcggt gcacacgtcg
     6181 ccggaccggc tccgtgagct gtgcgatcga cacggcgtcc cccacgaccg cacctacggt
     6241 cccggcaagc tgctcctcga gctgtacgag aagacgaccg aggcgacact gtgggacccg
     6301 accttcgtcg tcgactaccc cgccgaggtc tcgccgctgt cgcgccgcca ccgcgacgac
     6361 ccggagctga cagagcgctt cgaggccatc gtcgtgggtc gcgagctgtg caacgggttc
     6421 tcggagctga ccgaccccga cgaccagcgg tcccgcttcg aggaccaggc cgcgcgcaag
     6481 gccgccggcg acgccgaggc gatggcggtc gacgccgact acctccgggc cctcgactac
     6541 ggcctgccgc cgacggttgg actcggcatc gggatggacc gactcgtcat gctgctgtcg
     6601 ggcacctcgt cgatccgtga cgtggtcctc ttccccactc tgcgcccgga gccgcccgag
     6661 cgtgagcgtc ccgactcccc gtccggcgag tcggtcgagt cggggagctg acggggtggg
     6721 gagcctcttc ctcgacttcc tgggcgagga gatccaggtc gaaccgggcg agacgctgac
     6781 cttcggccgc gccgcggatc tgatcgtcga cgccgacaac cagtacctgc accgggtgct
     6841 cggctgcttc gccgcccagg gtgacgtctg gttcctgcag aacctggggc gccacatcac
     6901 cttgcgggtc atcgaccgcg acggttcgtc gaggaccgag ctcgccccat cggaccaggc
     6961 cccgatcggc ttcgaggagt tcgtcgtcgc cttcgacgcc ggtcccgcca gctacgagat
     7021 cggcggcgcg ctcgccgagc cgaccccgct cgagctgggg gtgtcggtcg cgtccgacac
     7081 cgtcgagttc gccaccatca acctcaacac cgagcagcgc atgctggtcg ccgcgctcgc
     7141 cgagccgctg ctgcggggtc gcaccgactg gcagtccgag atcccgagca acaaggagat
     7201 cgcccgccgg ttggggtgga ccgtctccaa gttcaaccgc aagctcgact acctgtgtcg
     7261 tcgcctggct gagcagggcg tcgacggcat gcagggcgag tcgggcgtgc tcgccaccag
     7321 ccgtcgggcc cgcctggtcc gccacatggt cgaccgccgc ctggtcgtgg tggcggacct
     7381 ggacgcgctg cctgcgcccc gctgacggct cgatcccacg acggcgtgtc ccgtaccctg
     7441 gtcggccgtg gccgtgggtg gggacgacgt cgaggtgccg ggggtcgtcc tcgaccgcgt
     7501 gatcggtcgg ggcgcgtcga gcgtggtgta cctgggcacc cagacccgct tcgaccgacc
     7561 cgtggcggtc aaggtcctcc acctgcccgg tcagaccgag ctggtcggaa agctgttcct
     7621 caacgagtgc cgcaccctgg gtcgcctgtc gcctcatccc aacgtggtgt cggtgttcga
     7681 cggcggcttc atcgacgacg agcggccgtt cctcgtcatg gagtacctgc ccgggggcac
     7741 gcttgccgac gagctggtcg ccggcggtcc gatcccgttc gccgaggtgc tccgggtcgg
     7801 ggtgcaactc gccggcggcg tgcactcggt ccacctgcac gggatcatcc acggcgacat
     7861 caagccggcc aacgtgctgc gcacccgcac cggtgacgtc gctctggccg acttcggcat
     7921 cgcccggctg atgtcgtcga ccgccaccac ccgggcgccg ctgctcacgc cgctgcacgc
     7981 cgcccccgag ctgttcgagg gcggtgcgat cacgccccgc accgacgtgt acgagctgtg
     8041 ctcgacgctg ttcgagctcc tcgacggtcg ggcgtcgttg ggcgagccga ccgacagccc
     8101 cctgctggtg ctcggccgca tggcgcgggg cgaccgtcga cgcctcgacc gcggccgggt
     8161 gcccgatgtg gtcgccacgg tcgtcgaagc gggcctctcg accgatccgg accaacggcc
     8221 cgccaccgct ctggagttgg gggagcagct tcgcagcgcg cagcggaccc tgggtctgga
     8281 ccccaccccg ctggtggtga tcgaaccgct cgactccgac gagtccgtgc aggatccccg
     8341 gccggccatc gatcacgggc cgtcggcacg ctcgtccgct gcagggaccg ccccgtcccg
     8401 aacggcgggg gtcgaggccg ggcgggccgg agacaccctg ccccctccgg atcgacgtcg
     8461 ctccctccgc ccgctggccc tggccgtggc agtggttgtc gccgccgtgc tcgtcggcgt
     8521 ggtcgtgttc gccctcgtgc ggggcggtga cgacgggacc gaggtgacga cgtccgggtc
     8581 cacctccacg acctggcccg aagaggtccg ggggaccccg agtgccgggt tccagccggg
     8641 tatcgagttc aacgttccgg gcgccagcga ccgtaacgcc gaggtgaacg cccgcctcgg
     8701 ggacgggtcc caggtcctcg gcacgttcgg atcgggcgtg gtcgtgtccg accagccgcc
     8761 gccgttcgct gcgcagctgc ccgtccggtt ccgctggcag gcgttcaacc cgcagctgga
     8821 cgaggactgc ctggcgatcg tctcctggcc gctcaccgtc accggatggt gggagaagcg
     8881 ggcgacgtgg tcgggacacc aggcggtcgt ccgggtcctc aagttcgctt cggagaggga
     8941 tgccgtcgag gcgttcaacg tcttctccct cgagcagggg gtcaccggcg acgactgcca
     9001 cgggttccgc tccgacggaa agccgttcga ccacgaccgg atcgaggtcg cgcaccgggc
     9061 ggtcgacctg ggcctgggcg acgacgtccg ctggaacagc ttcctggccg gggccccgtc
     9121 ccaacccgcg ggcttcacct cgtccaccaa cgccatcgcc cagaagggcg acgtcgtcgt
     9181 cctgggcaac gtggccaacg cggacacccc ggtcgatccc gccaccttcg gggcgacctt
     9241 ctcctcgatc ctgaccgccc tgtcggcctg accgcccctc gatcc
//
LOCUS       DAOQOD010000123         9177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2770_length_9177_cov_10.048674, whole genome shotgun sequence.
ACCESSION   DAOQOD010000123 DAOQOD010000000
VERSION     DAOQOD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2770_length_9177_cov_10.048674"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLZ72_11565"
     CDS             complement(<1..205)
                     /locus_tag="PLZ72_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36979.1"
                     /translation="MFKRLTWFGIGAAAGATGFVWAQQRVRRELDALGPDHLAVVAAH
                     QAGRVTRQAARTVAGAVSDGRSAM"
     gene            complement(244..636)
                     /locus_tag="PLZ72_11570"
     CDS             complement(244..636)
                     /locus_tag="PLZ72_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36980.1"
                     /translation="MSTLDVVVILLGIVALGAAVALTLATVRMRRVTAEAELLVEDLQ
                     RTRAAFEEDAAAAFDELHRTVVRAGAQVDEVEALVDVAHAIGGRVDAATDVTYRALTS
                     PVIKTVALASGTRRAARRLRPGRHDDPS"
     gene            complement(633..1985)
                     /locus_tag="PLZ72_11575"
     CDS             complement(633..1985)
                     /locus_tag="PLZ72_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNA36981.1"
                     /translation="MADDLFAAAAQDRLAARGPLADRLRPRTLDDVVGQDHLLGAGRP
                     LRALVDADRLSSVILWGPPGTGKTTIARLIATASSKAFVPLSAVTATVKDIRDVTAGA
                     EQRLGGHGQGTILFLDEIHRFTKSQQDALLPSVETGLVVLIGATTENPHFEVNPPLMS
                     RSTLFRLHALDQGALTTLAERGLAAESATADDDAVEHLVQRCGGDGRHLLTSLEVAVA
                     LAAGRTRTAGATAVHVELVDAEGALGASPVRYGRDDHFDVVSAFIKSIRGSDPDAALH
                     WLARMLDAGEDARFIARRLVISASEDVGLADPLALVIADAAARAVEFVGLPEARINLA
                     QVTVHLALAPKSNTAYAGLEAATSDVRRRPVGEVPAHLRDAHYQGAASLGHGSGYRYP
                     HDHPDGWVAQQYLPDELADARYYRPGANGAEGRLVARWRERRGDIPAPDPDAEQTGTP
                     "
     gene            complement(2079..3149)
                     /locus_tag="PLZ72_11580"
     CDS             complement(2079..3149)
                     /locus_tag="PLZ72_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36982.1"
                     /translation="MTTFRPARALRRGALALTLGLGLVASACAPEPTPPTPNQAAAHN
                     AGVWMNSQFTAGHAFPGPFGGESAVSAAEAIADLDALGVGQAQQADRLARLEADTPAA
                     ITDGTNDVPGNLARIILAVVATGGNPRAFAGLDLVARLEATVGVDGRFGAQSPLYDGV
                     YRQGLALAALSVVTPRPASITPGAGQSLADLPAVAWLADQQCSDGSWMAYRADTSAPC
                     VEDPATWTYKDSNAAAMATLGLTAIGATAPVDPTAWFQSVRGTDGAWGSFPPAPTTPS
                     DADSTGLVIAALEALGHVPDATAYATLRSFQIGGTAPVANHGAFVYQWPWDASPAVPS
                     IMATFDSVTALFDETWPQALVP"
     gene            complement(3246..3779)
                     /locus_tag="PLZ72_11585"
     CDS             complement(3246..3779)
                     /locus_tag="PLZ72_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36983.1"
                     /translation="MTTPQSTISGTRRVRFVGAALAAVAVVLLSACAPPTGPTTPGQT
                     YKEGRCAADEGVTVVVDFAPLQDRVVVRCALGAQPSGTGALAAVNLPVDPGPITGGVC
                     KIDGLPTQGYPYCWTTGGYWSYWKAATAGAPWSYSTVGAAANAIPAGAVEGWRFAAFS
                     AGTAVPPRVGTSGPIVP"
     regulatory      complement(3875..4027)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4075..5880)
                     /gene="aspS"
                     /locus_tag="PLZ72_11590"
     CDS             complement(4075..5880)
                     /gene="aspS"
                     /locus_tag="PLZ72_11590"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNA36984.1"
                     /translation="MSTPSAPEQATALRTHLCGVLRPEHVGQTVTVCGWAARRREHGE
                     HLAFIDLRDHSGLLQCVVDGAHDIRSEYVLAITGTVRPRPEGTVNEALATGEVELGEC
                     SVEVLSVAEPPPFPLDDRAADVDETVRLRHRYVDLRRDDMQRNLRTRAAVNRAIRAAM
                     DDQGFVEVETPMLIASTPEGARDFVVPSRQKPGNFYALPQSPQLFKQLCMVGGVDRYY
                     QIARCFRDEDLRADRQFEFTQLDAEMSFASQEDVLVAITAAVSAATAAVTGDEVTDVP
                     RITWRDAMDRYGSDKPDVRFGMELVELTDVFAATGFNAFKASCIKGIRLEGGSESTSR
                     SRLDDLTDTAKRWGAKGLVWMRVERGEDGALSLNSPIAKFLSEAELAGIAQRLGAAEG
                     DLLFLVADDWATTCHVLGLLRLELGRPPVDEGGRRLLWVVDFPLFESIDPATGRPVSA
                     HHPFTMPHAEDLGLLAEVASGDGDAESLLAVRSQAYDLVLNGWELGSGSVRIHRPDVQ
                     QQIFGLLGISEAEAQHKFGFLLDAFRFGAPPHAGFAFGIDRLVALLVGEENIREVIAF
                     PKTQSGADPLTKAPTAIDAVQLDELGLRLLPPPAD"
     gene            complement(5877..7142)
                     /gene="hisS"
                     /locus_tag="PLZ72_11595"
     CDS             complement(5877..7142)
                     /gene="hisS"
                     /locus_tag="PLZ72_11595"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNA36985.1"
                     /translation="MTDAPPHGFRALTGMRDVLAPESSRRRVLVGQFADVVEAAAYQE
                     LVLPLVEDLGVFLRVGEATDVVTKEMYDFVDKDGSRIALRPEMTAGAVRAFVQHSPVL
                     PWKVWYSGSNFRHERPQKGRYRSFDQVGVEVLGVDDPDLDVEVVALGWRFFERLGLRD
                     VRLLVNSLGEHADRARYSEAVRSHLESRRGDLSEKALETLARNPLRVLDSKRPEDRAV
                     VADAPRVADHLSDESAAHFARVLAGLDALRIPYTVEDRLVRGLDYYRRTTFEYVADSL
                     DSAQNAIGGGGRYDRLAEDLGGPPTDGIGFALGVDRTLLACDAEGVFPAPARALDAFV
                     VDLVGGDAARDLTHALRAAGLSADRRFGAGSMKAQMKAADRSGARFALLVGSDELDAG
                     EVTVRDLREGGEQRRVARDHLIDDLRTHL"
     gene            complement(7208..8455)
                     /locus_tag="PLZ72_11600"
     CDS             complement(7208..8455)
                     /locus_tag="PLZ72_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36986.1"
                     /translation="MSPRPERSKADRTKPDRPKATRSKGGRKAERTEPIEPERTELHL
                     VDSDAGHAPAVVGDSGADGGAVVTTSGSPVQDARDLLASTDPVAYYKALSGLARQGHH
                     AVLEPLPYRSNLDFHDPTCVVVDPRPANTDTIELFANAAYPDFPWLLSRFRDPVVAER
                     LDAVYQLVFNEGANVAVVTNHGQIIDIALVGGAMIDAMCSAGRAYGVLGEQIEVDDLA
                     ERFNVLVSRMVTTRMAFNVPAMQVLQVGARTFLSVPQTHSRRRSKLDPALARANNQLM
                     RHELDLQLSEGGQILAMAASGSQDLSVAVEMVQKIKAQWRQMRGAEPDDTAETVHLQP
                     LYDGTMNMMLGCRFVLPIAVSLDPTHPALEVGSLTRVRQKDDCHRIMEWIADAHQQAT
                     GLPTLYHRQEDDLLTQVRAVVKT"
     gene            complement(8570..>9177)
                     /locus_tag="PLZ72_11605"
     CDS             complement(8570..>9177)
                     /locus_tag="PLZ72_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HNA36987.1"
                     /translation="RACDSGWCAGEWAGGGSGAKGGGGGQGGAGGTAGTNGGGSFAVY
                     SHNSTVTIDANTTLTAASGGNGGAGAAGQNGAPGGSGGKGGNGIIADCCTGSPGSGGN
                     GGAPGSAGAAGTNGAAGTCCNAASGGAGGGGGGGNGGKGGTGGGGVGGPSVAMAKVGT
                     GSITFGGDLGSQVTIGSAGSGGAAPGGNAGAAGTAAKQASF"
BASE COUNT         1232 a         3176 c         3520 g         1249 t
ORIGIN      
        1 gcatggccga ccgcccgtcg gacaccgctc cggctacggt ccgggccgcc tggcgggtga
       61 cccggccggc ctggtgggcg gcgacgaccg ccaggtggtc aggcccgagc gcgtcgagct
      121 cgcgacgcac gcgctgctgg gcccacacga atccggtggc gccggcggca gccccgatcc
      181 cgaaccaggt cagacgcttg aacaccgcac cagtctgccc gacccgcagc ggcggcgcgg
      241 gaatcagctc ggatcgtcgt ggcgtccggg tcgcaggcgg cgcgcggcgc ggcgcgtgcc
      301 cgaggccagg gcgacggtct tgatcaccgg cgaggtcagt gcccggtagg tgacgtcggt
      361 cgcggcgtcg acccgaccgc cgatggcgtg ggccacgtcg accagcgctt cgacctcgtc
      421 gacctgggcc ccggcgcgca cgacggtccg gtgcagctcg tcgaacgcgg cggcggcatc
      481 ctcttcgaag gcggcccggg tccgctgcag gtcctcgacg aggagctcgg cctcggcggt
      541 gacccgacgc atccgcaccg tggccagcgt caaggccacc gcagcgccca gagcgacgat
      601 ccccagcagg atcacgacca catcgagggt gctcatgggg tcccagtctg ctcggcgtcg
      661 ggatccggtg ccgggatgtc gccccgacgt tcccgccagc gggccaccag ccggccctcg
      721 gctccgttgg cgcccggacg gtagtagcgg gcgtcggcca gctcgtcggg aaggtactgc
      781 tgggccaccc agccgtcggg gtggtcatgc gggtagcggt agccgctccc gtgtccgagc
      841 gacgccgcac cctggtagtg ggcgtcgcgc aggtgcgccg ggacctcacc gaccgggcgg
      901 cggcggacat cggaggtggc cgcctccagg ccggcgtacg cggtgttgga cttcggtgcc
      961 aacgccaggt gcaccgtgac ctgagccagg ttgatgcgtg cctcgggaag gccgacgaac
     1021 tcgacggccc gggcggcggc gtccgcgatc acgagggcga gcgggtcggc caacccgacg
     1081 tcctcggacg cggagatgac caggcgtcgg gcgatgaagc gggcgtcctc gccggcgtcg
     1141 agcatccggg ccagccagtg cagggcggcg tcggggtccg acccgcggat cgacttgatg
     1201 aacgccgaga ccacgtcgaa gtggtcgtcg cgcccgtagc ggaccggtga cgcgccgagc
     1261 gctccctcgg cgtcgacgag ctcgacgtgg accgcagtcg cacccgcggt gcgggtccgg
     1321 ccggcggcca gggcgaccgc gacctcgagg ctggtcagca ggtggcgtcc gtccccgccg
     1381 cagcgttgca cgaggtgctc gacggcgtcg tcgtccgcgg tggccgactc ggcggccaga
     1441 ccccgctcgg ccagcgtcgt caacgcgccc tggtcgagcg cgtggagccg gaacagcgtg
     1501 gagcggctca tcagcggcgg gttgacctcg aagtgcgggt tctcggtggt ggcaccgatg
     1561 agcaccacga gcccggtctc gaccgacgga agcagggcgt cctgctggga cttggtgaac
     1621 cggtggatct cgtcgaggaa caggatcgtg ccctggccgt ggccgcccag gcgctgttcg
     1681 gcaccggcgg tgacgtcgcg gatgtccttc accgtggcgg tcaccgcgga cagcgggacg
     1741 aacgcctttg acgacgcggt ggcgatcagc cgggcgatcg tggtcttgcc ggtgccgggc
     1801 ggcccccaga ggatcaccga ggacagccgg tcggcgtcga ccagcgcccg caagggccgt
     1861 cccgctccga gcaggtggtc ctggccgacg acgtcgtcga gggtgcgcgg ccgcagtcgg
     1921 tcggccagcg ggccgcgggc ggccagccgg tcctgcgccg cggcggcgaa caggtcatca
     1981 gccatgggcg ccgacgctac ccactgccga caccgcgcca ggtccgtgat gggttccacc
     2041 gggatctccc ggtggaaccc atcacggcgg tcctggggct acgggacgag ggcctgcggc
     2101 caggtctcgt cgaacagggc ggtcaccgaa tcgaacgtcg ccatgatcga cgggacagcc
     2161 gggctggcat cccacggcca ctggtagacg aacgcgccgt ggttcgcgac gggggcggtc
     2221 ccgccgatct ggaagctgcg cagcgtggcg taggccgtcg cgtcggggac gtggccgagg
     2281 gcctccagcg cggcgatcac cagtccggtc gagtccgcgt ccgacggcgt cgtcggcgcg
     2341 ggcgggaagg aaccccaagc gccgtcggtg ccgcgcaccg actggaacca ggcggtcggg
     2401 tcgaccgggg cggtggcgcc gatcgcggtc aggcccaggg tggccatcgc cgcggcgttg
     2461 gagtccttgt aggtccacgt ggcggggtcc tcgacgcacg gggcggaggt gtcggcccgg
     2521 taggccatcc acgagccgtc gctgcactgc tggtcggcca gccacgcgac cgcgggcagg
     2581 tcggccaggc tctgacccgc tcccggggtg atcgacgccg ggcgcggcgt caccaccgac
     2641 agcgccgcga gggccaggcc ctggcggtac acgccgtcgt agagcgggga ctgggcgccg
     2701 aaccgaccgt cgacgccgac ggtggcctcg agccgggcga ccaggtccag accggcgaac
     2761 gcccgcgggt tcccaccggt ggcgaccact gcgaggatga tgcgggccag gttgccgggc
     2821 acgtcgttgg tgccgtcggt gatggcggcc ggggtgtcgg cctcgaggcg ggccaggcgg
     2881 tcggcctgct gggcctgacc gacgcccagg gcgtcgaggt ccgcgatcgc ctcggccgcg
     2941 ctcaccgcgc tctcaccgcc gaaggggccg gggaacgcgt ggccggccgt gaactgggag
     3001 ttcatccaga ccccggcgtt gtgcgccgcg gcctggttcg gggtcggcgg agtgggctcc
     3061 ggcgcgcacg ccgaggcgac caggccaaga ccgagggtca gggcgagggc gccgcggcgc
     3121 agggcccggg cgggacggaa ggtggtcatg ggtggtttct cctcagctgt tcgtggtggt
     3181 ctgggtcctg cgtcgaccgg gaaccggccg gcgggcgcag gatgtcggct gtccggtcgt
     3241 ggcgatcagg gaacgatcgg gccggaggtg ccgacgcggg gcggaacggc ggtgccggcg
     3301 ctgaacgcgg cgaagcgcca gccctcgacc gctccggccg ggatggcgtt ggccgcagcg
     3361 ccgaccgtcg agtacgacca gggcgctccg gcggtggcgg ccttccagta ggaccagtag
     3421 ccgccggtgg tccagcagta cgggtagccc tgggtcggca ggccgtcgat cttgcagacg
     3481 ccaccggtga tcgggccggg atcgaccggc aggttgaccg cggcgagcgc gccggtgccg
     3541 ctgggctggg cacccaaggc gcagcggacc acgacccggt cctggagggg ggcgaagtcg
     3601 acgacgacgg tgacgccctc gtcggctgcg cagcgaccct ccttgtaggt ctgccccgga
     3661 gtcgtcggcc cggtcggggg cgcgcaggcc gaaaggagca cgaccgcgac ggccgcgagg
     3721 gcggcgccga cgaaccggac gcggcgcgtg cctgagatgg tcgactgcgg tgtggtcatc
     3781 ctgatcgcct ccggcgtcgg tcgacgcgga cggcggatcg gatccgacgg gtcgaggacc
     3841 cggcagatgc tccacccgca gtccacgacg ggtctcttcg agctcgtcgc cgggcgggtg
     3901 ctccgactcg tgcgacgaat ggccgaccgg ccgttcgtcg cacccacggt tgcgggacag
     3961 tgccggattc ggaccggctt tccccgatgg gcgacgtgtg gcggcgaccc tagccgcgct
     4021 ccgatcctgc ccgcacctcc gggcctggcg atctcgtgct ccggctgggc gggctcagtc
     4081 cgcgggcggg ggcaggagcc gtagcccgag ctcgtcgagc tgcactgcgt cgatggcggt
     4141 cggcgccttg gtcaacgggt cggctcccga ctgcgtcttg gggaaggcga tgacctcgcg
     4201 gatgttctcc tcgccgacga gcagcgcgac cagacggtcg atgccgaacg cgaagccggc
     4261 gtgcggcggg gcaccgaagc ggaaggcgtc gagcaggaag ccgaacttgt gctgggcctc
     4321 ggcctcggag atgccgagca acccgaagat ctgctgctgc acgtcgggac ggtggatacg
     4381 gacgctgccc gagccgagct cccagccgtt gagcaccagg tcgtaggcct gggagcgcac
     4441 ggccagcagc gactcggcgt cgccgtcgcc ggacgccacc tcggccagga gcccgaggtc
     4501 ctcggcgtgc ggcatcgtga acgggtggtg cgccgagacc ggtcggccgg tggcggggtc
     4561 gatcgactcg aacaacggga agtcgacgac ccacagcagc cggcgaccgc cctcgtcgac
     4621 ggggggtcgg ccaagctcca gtcgcagcag gccgagcacg tggcacgtgg tggcccagtc
     4681 atccgcgacc aggaacagca gatcgccctc ggcggcgccg agtcgctgcg cgatcccggc
     4741 gagctcggcc tcggagagga acttggcgat cgggctgttc agcgacaggg cgccgtcctc
     4801 gccgcgctcg acccgcatcc acaccaggcc cttggccccc cagcgcttgg cggtgtcggt
     4861 gaggtcgtcg aggcggctgc ggctggtcga ctccgatccg ccctccaggc ggatcccctt
     4921 gatgcacgac gccttgaacg cgttgaaccc ggtcgcggcg aacacgtcgg tgagctcgac
     4981 cagctccatc ccgaagcgca cgtcgggctt gtcggagccg tagcggtcca tggcgtcgcg
     5041 ccaggtgatc cgcggcacgt cggtgacctc gtcgccggtg accgccgcgg tggcggcgct
     5101 gaccgcggcg gtgatggcga cgagcacgtc ctcctgggag gcgaagctca tctcggcgtc
     5161 gagctgggtg aactcgaact ggcgatcggc gcgcaggtcc tcgtcgcgga agcagcgggc
     5221 gatctggtag tagcggtcga cgcctccgac catgcagagc tgcttgaaca gctgcgggct
     5281 ctgcggcagc gcgtagaagt tgccgggctt ctggcgtgac ggcaccacga agtcgcgggc
     5341 gccctcgggg gtggacgcga tgagcatcgg cgtctcgact tcgacgaagc cctggtcgtc
     5401 catcgcggcg cggatggccc ggttgacagc ggcgcgggtg cggaggttgc gctgcatgtc
     5461 gtcgcgacgc aggtcgacgt agcggtgccg gagccggacg gtctcgtcga cgtccgcggc
     5521 gcggtcgtcg agcgggaacg gcggcggctc cgccaccgag agcacctcga cgctgcactc
     5581 ccccagctcg acctcgccgg tggcaagcgc ctcgttgacg gtgccctcgg gccggggccg
     5641 gaccgtcccg gtgatcgcca ggacgtactc gctgcggatg tcgtgggcgc cgtcgacgac
     5701 gcactgcagc agtcccgagt ggtcacgcag gtcgatgaag gccaggtgct cgccgtgctc
     5761 gcggcggcgg gccgcccagc cgcacacggt gacggtctgc ccgacgtgct cgggccgcag
     5821 caccccgcac aggtgggtgc gcagtgcggt ggcctgctcg ggggcggagg gggtgctcac
     5881 aggtgggtcc tcaggtcgtc gatcaggtgg tcacgggcga cgcggcgctg ctcgccgccc
     5941 tctcggaggt cgcggacggt cacctcgccg gcgtcgagct cgtcgctgcc gaccagcagg
     6001 gcgaagcggg cgcccgagcg atccgcggcc ttcatctggg ccttcatcga gccggccccg
     6061 aagcgacggt ccgcggacag cccggcggcc cgcagggcgt gggtgaggtc gcgggcggcg
     6121 tcgccgccga ccaggtcgac cacgaaggcg tcgagtgcgc gtgcaggggc cgggaacacg
     6181 ccttcggcgt cgcaggccag cagcgtccgg tccacgccga gggcgaaccc gatgccgtcg
     6241 gtgggcggac cgcccaggtc ctcggccaac cggtcgtagc ggccgccgcc accgatcgcg
     6301 ttctgggccg agtcgagaga gtcggccacg tactcgaagg tggtgcgccg gtagtagtcc
     6361 agtccccgca ccagccggtc ctcgacggtg tacgggatgc gaagcgcgtc gagcccggcg
     6421 aggacgcggg cgaagtgcgc cgcggactca tcggagaggt gatccgccac gcggggggca
     6481 tccgcgacga cggcgcggtc ctcggggcgc ttggagtcga ggacccgcaa cgggttgcgg
     6541 gccagggtct cgagcgcctt ctcggacagg tcgccccgcc gggactcgag gtggctgcga
     6601 acggcctcgc tgtagcgggc ccgatcggcg tgctcgccca ggctgttgac gagcaggcgg
     6661 acgtcgcgga gacccaggcg ctcgaagaac cgccacccga gggcgacgac ctcgacgtcc
     6721 aggtcggggt cgtccacccc gaggacctcg acgccgacct ggtcgaagga ccggtagcgc
     6781 cccttctggg ggcgttcgtg gcggaagttg ctgcccgagt accagacctt ccagggcaga
     6841 accggcgagt gctggacgaa ggcccggacc gcgcccgcgg tcatctcggg gcgcagggcg
     6901 atgcgcgacc cgtccttgtc gacgaagtcg tacatctcct tggtgaccac gtcggtggcc
     6961 tcgccgacac gcaggaagac gcccaggtcc tcgaccaggg gcagcacgag ctcctggtag
     7021 gcggcggctt cgaccacgtc ggcgaactga ccgaccagca cacggcgacg cgacgactcc
     7081 ggcgccagga cgtcgcgcat tccggtgagg gcacggaatc cgtgcggggg cgcgtcggtc
     7141 acggccggcg aggttaccgg agcgggcccg ccgctccgga accggttcgg gcgccctcga
     7201 ggcgtcgtca ggtcttcacc accgcccgca cctgggtgag caggtcgtcc tcctggcggt
     7261 ggtagagggt cggcagtccg gtggcctgct ggtgcgcatc cgcgatccac tccatgatgc
     7321 ggtggcagtc gtccttctgg cggacccgcg tgagcgaacc gacctcgagc gccgggtgcg
     7381 tcgggtcgag cgacacggcg atgggcagca cgaagcggca gccgagcatc atgttcatgg
     7441 tgccgtcgta gagcggctgc aggtggaccg tctcggcggt gtcgtcgggt tcggcaccgc
     7501 gcatctgacg ccactgggcc ttgatcttct gcaccatctc gaccgcgacc gacaggtcct
     7561 ggctgccgct ggcggccatg gcgaggatct ggccgccctc cgagagctgc aggtcgagct
     7621 cgtggcgcat cagctggttg ttggcccggg cgagggcggg gtcgagcttg gaccgacgcc
     7681 gcgagtgggt ctggggcacc gacaggaacg tccgggcgcc gacctgcagc acctgcatgg
     7741 ccggcacgtt gaacgccatg cgggtggtga ccatgcggct gaccaggacg ttgaaccgct
     7801 cggccagatc gtccacctcg atctgctcgc ccagcacccc gtaggcgcgt cccgccgagc
     7861 acatggcgtc gatcatggcg ccgccgacca gggcgatgtc gatgatctga ccgtggttgg
     7921 tcaccaccgc cacgttcgcg ccctcgttga acacgagctg gtacaccgcg tccaggcgct
     7981 cggcaacgac cgggtcccgg aaccggctga gcagccacgg gaagtcgggg tacgccgcgt
     8041 tggcgaacag ctcgatggtg tcggtgttgg ccgggcgggg gtcgaccacg acgcaggtcg
     8101 ggtcgtggaa gtccaggttg gaccggtacg gcaggggttc gagcaccgcg tggtgtccct
     8161 ggcgggccag tcccgacagc gccttgtagt aggcgacggg atcggtggag gccagcaggt
     8221 cgcgagcgtc ctggaccggc gatcccgatg tggtcacgac agcaccgccg tcggcaccgg
     8281 agtcgcctac gaccgccggc gcgtggccgg catcgctgtc gaccaggtgg agctcggtcc
     8341 gttccggctc gatcggctcg gtgcgctcag ccttgcggcc gcccttggag cgggtggcct
     8401 tgggccgatc gggcttggtt cgatcggcct tggatcgttc ggggcgtggg ctcacgccgc
     8461 cattctggtc ccgccggcgc cgaactcgcg cccgaacgcg gttcgggccg ccgtcccgtc
     8521 cgccgaggcg ggggacgacg gcccgatggg tcgtcaggga ttcggacggt cagaacgacg
     8581 cctgcttggc cgcggtgccc gccgctccgg cgttgccacc gggcgctgcg ccgccgctgc
     8641 ctgcggaacc gatggtgacc tgactgccca ggtcgccccc gaatgtgatg gacccggtgc
     8701 cgaccttggc catggcgacc gacggaccgc cgacgccacc gccgccggtg ccgcccttgc
     8761 cgccattgcc gccgccgccg ccaccgccgg caccgccgga ggccgcgttg cagcaggtgc
     8821 cggctgcgcc gttggtgcca gcagctccag cgctaccggg ggcgccgccg ttgccgccgg
     8881 aacccgggga accggtgcag cagtcggcga tgattccgtt gccacccttg ccgccggagc
     8941 cacccggtgc accgttctgg cccgcagccc cggccccgcc gttgccgccg ctggccgcgg
     9001 tgagggtggt gttggcgtcg atggtcaccg tcgagttgtg gctgtagacg gcgaacgatc
     9061 cgccgccgtt ggtgccggcg gtgccaccgg caccaccctg gccaccgccg ccgcccttgg
     9121 caccggaacc gccgccggcc cactcgccag cgcaccagcc gctgtcacag gcgcggc
//
LOCUS       DAOQOD010000124         9138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2791_length_9138_cov_11.918419, whole genome shotgun sequence.
ACCESSION   DAOQOD010000124 DAOQOD010000000
VERSION     DAOQOD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_9138_cov_11.918419"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..939)
                     /locus_tag="PLZ72_11610"
     CDS             complement(130..939)
                     /locus_tag="PLZ72_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA36988.1"
                     /translation="MTVFVTGAGSGIGLHLARLALERGDRVAGFDLRIADDVGSELRD
                     RARSADRVWLRDAVDVRDTSAVASAFDDAVAQIGSPTLVINSAGISTNDLFAASSAED
                     FERTVAVNLFGSRNVAAAALPHLRRGSRLALIASLAGITGGYTYAAYAASKAGVIGLA
                     RVLRLEWAPDGIGVSVICPPEILTPMVEQYATTMHPATRALKDVAGTLPIDEACREMV
                     AQLDRGRFMVIPGRRARRAARMTRLLPEAVTTRIADRIVRRALAEPPPTNL"
     gene            1101..>1538
                     /locus_tag="PLZ72_11615"
     CDS             1101..>1538
                     /locus_tag="PLZ72_11615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNA36989.1"
                     /translation="MNASPVRTRPRGQGGFTLLELLVVVGTLSVMSGAAVVGVGSMRA
                     NAQRAACDADADTISTAEEAALIQGSYLSEADLVAKGFLADESTRWDVTVSGASFQLV
                     PIGDCVPDGTEEAAGTTPTTVDPLAKQKAEQAARDKAKAEALAK"
     assembly_gap    1539..1638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1701..2120)
                     /locus_tag="PLZ72_11620"
     CDS             complement(1701..2120)
                     /locus_tag="PLZ72_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HNA36990.1"
                     /translation="MAATATARWEGTIDEGTGAVSTGTGLTGTFTKASRFADGAGTNP
                     EELIGAAHAGCFSQFLALLLTKNETPPTSIETTAKVSIAVTDEGPTITRIALSTTVDV
                     DLPAEKIDELAQNAKANCPVSKALAGVGDVSLTVTKA"
     gene            complement(2227..2880)
                     /locus_tag="PLZ72_11625"
     CDS             complement(2227..2880)
                     /locus_tag="PLZ72_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA36991.1"
                     /translation="MDDDVQVQARALGSPTRHAVFRYLVDADRPVGVAELTEQFGFNH
                     NAIRQHLAKLVDAGLVTEAQAPPSGRGRPRLEYTVAPTTESRWGAIGSYEMLSTMLTE
                     IIRSGDTPREVGRRVATGLGHEVADGRDATTVLVDQMSRQGFSPRVRVHGDAVEITAQ
                     ECPFPSAVLTDSDTICQLHLGMAEGIADAVGGLTIEELVTKDPRRAHCRLKCRTDVD"
     gene            complement(2966..3268)
                     /locus_tag="PLZ72_11630"
     CDS             complement(2966..3268)
                     /locus_tag="PLZ72_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36992.1"
                     /translation="MTEMGPTDGTKITPTQAESLTALSEPWLSCDDCFEQIDLYVDSL
                     VHDHPHLDEPLRVHLARCPACMEEAESLISLVAGEDRSDEVDLLAAFRADVGSVGG"
     gene            complement(3265..3918)
                     /locus_tag="PLZ72_11635"
     CDS             complement(3265..3918)
                     /locus_tag="PLZ72_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA36993.1"
                     /translation="MTGSDTPTATGAPTIPTDDCDWIDALRGDGPGHHAAVRRLHDTV
                     HRAAAHQVRRMPHVWSQLGAVRAQEIIDAAADEATTAVLGSLDRFEGRSRFSTWVFKF
                     GILHAGTEARRALWRDRPVDLTEHPEPATGDHDGSASVVEARDLAAAVSTAIDTVLTA
                     RQRRVAIALIVDEVPIDVLAERMGTNRNALYKALHDIRVKLRSEMREQGYIHDEHRR"
     gene            complement(4104..4814)
                     /locus_tag="PLZ72_11640"
     CDS             complement(4104..4814)
                     /locus_tag="PLZ72_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36994.1"
                     /translation="MTPTSRPNRVFASVATVVTAATFLVACGPGSAGPVSAPPVGNHG
                     GHGTETSAPAERPTRSDLRSGMRELWSDHMQWTYATVDSFFHNPSAVQPTLDRLLRNQ
                     SDIGNAIKPFYGTKAGDALTALLTAHINGAVPVLQAAQSGDQAALKSALDAWYANAKE
                     IADFLSAANPKHWPTSATEPMMKGHIDQTTAYATDLLKGDHASAIEHYDEAYDHMMML
                     ADTLAQGVAAQFPNKVRR"
     gene            5103..6194
                     /locus_tag="PLZ72_11645"
     CDS             5103..6194
                     /locus_tag="PLZ72_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36995.1"
                     /translation="MFLALRDLRRGARRFALLGVVIALVALLSTVLSALATGLVTDGI
                     SGLRALPFDHLALQDGSESTFSRSTLKSDALEKFEGLDGVKATPLGASFVNAASTDGG
                     PNLDLALFGVSPDSFLVERPEARAALDGPPGLVLAHELEEQGVKVGDTYTLGGSDVEL
                     PVLGFTFAGTYGHAPIAFTSLETWQKVQFGDGADGRFAAVALTGGTDPQLEKAATATH
                     TDVLTKKEAYAGSPGYTAETTTMSLIRGFLLVISALVIGAFFTVLTVQRTRQIGLLKA
                     MGATNGYILRDGIGQMAILVVLATAIGVALGCTLVFFMGRGDAPVELSVPAVTTVVVS
                     LVVAGVLGSLVAFKRITGVEPAIALGVEP"
     gene            6191..6874
                     /locus_tag="PLZ72_11650"
     CDS             6191..6874
                     /locus_tag="PLZ72_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA36996.1"
                     /translation="MTEPAVLLEGIRVAFPDGPDERVVLDDLKLSVDPGELVVISGQS
                     GAGKSTLLAVTGLLRRADAGEVTIAGTATSAMSSRQRTAVRRDHIAFVYQSANLLPSL
                     TAVEQLELVGHIRGERSADVRRRARGLLDELGLADRASQLPSQLSGGERQRVGIARAF
                     MAEPSVLIADEPTASLDPQRADSVAELLAGASARGIATIVVAHDTAALSRADRHLRME
                     DGRLLDVGG"
     gene            6879..7469
                     /locus_tag="PLZ72_11655"
     CDS             6879..7469
                     /locus_tag="PLZ72_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA36997.1"
                     /translation="MPRIKADNIAEHVAQQRAAVLDAAVRLFVERGFSEVGLADVAAE
                     VGLARNSLYRYVPDKTHLLVEWYRRTVPATIADWERATAGDDPAPVRLQRWARSYLEW
                     ALTPEHQLVGPLTDSVSGLDDDTRAEVAELHRSMMAVVARVVAEAGVSGDHVDGAVAL
                     LAGLVLGAARAEGGSGADDDLRRRLDNAVAAVVDTP"
     gene            7533..8918
                     /locus_tag="PLZ72_11660"
     CDS             7533..8918
                     /locus_tag="PLZ72_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA36998.1"
                     /translation="MTTTPSSAAPIAAVDPRTGRPLDVDALQCRTLRVVVASQICGGA
                     GLAAGVTVGALLAREMLGGEGFAGVPTALFTLGSALTAFLVGRVTQRAGRRVGLAAGF
                     GAGAIGAVGIVAAAVLYNVVLLLPSLLVYGAGTATNLQARYAGTDLAHADRRATAVSV
                     AMVSTTIGAVAGPNLVGPLGSFARSVGAPALAGPFVLGAVAYASAGTVLFLLLRPDPF
                     LVSRSLVDSERRSATDGSAATAGAAAAEHPEMPASVPSTEPVRPDVRVGSGAYLGAAV
                     MVITQMAMVAIMTMTPVHMRAHGHDIGDVGLVIGFHIGAMYLPSLVTGVLVDRFGRIT
                     MAVAAAVTLLAAGVTAATAPGESLPALVAALVLLGLGWNFGLISGTALVVDATSPEDR
                     PRVQGTIDVGVALAGAGGGVLSGVIVAGSSYATLSLLGGVLSLAVIPVLWWTRRMEAR
                     HRAQRGLTVPG"
BASE COUNT         1159 a         3167 c         3303 g         1409 t
ORIGIN      
        1 gactgtcagc tacaagttcg tcggcgttgg tcgaacttgt agcgctcggg tcgtcggggt
       61 gactgtcagc tacaagttcg tcggcgttgg tcgaacttgt agcgctcggg tcgtcggggt
      121 gactgtcagc tacaagttcg ttggcggggg ttccgcgagg gcgcggcgga cgatccgatc
      181 ggcgatccgt gtggtgacgg cctcggggag caaccgggtc atgcgtgccg cccgtcgcgc
      241 ccttcgtccc gggatcacca tgaaccgacc ccggtcgagc tgggccacca tttcccggca
      301 cgcctcgtcg atcggcagcg tgccggccac gtccttcagc gcccgggtgg cggggtgcat
      361 ggtcgtggcg tactgctcga ccatcggcgt caggatctcc ggcgggcaga tcaccgacac
      421 gccgatcccg tccggcgccc actcgagtcg cagcacccgg gccaggccga tcaccccggc
      481 cttggacgcg gcgtaggccg cgtacgtgta gccgccggtg atcccggcga gcgacgcgat
      541 cagagcaaga cgtgaacccc gacggaggtg ggggagtgca gccgcggcca cgttgcgact
      601 gccgaacagg ttcaccgcga cggtccgctc gaagtcctcg gcggacgagg cggcgaacag
      661 gtcgttcgtg ctgatccccg cgctgttgat caccagcgtc gggctcccga tctgcgcgac
      721 ggcgtcgtcg aacgccgagg ccaccgccga ggtgtcgcgc acgtccacgg catcgcgcag
      781 ccacacgcgg tccgctgagc gtgcccggtc acggagctcg ctccccacgt cgtcggcgat
      841 ccgcaggtcg aacccggcga cccggtcgcc ccgctcgagg gcgagtcggg cgaggtgcag
      901 cccgatcccg ctcccggctc cggtgacgaa gaccgtcacg tcccggtctc tcgatgcggg
      961 gtgtggttgc gcgggaccgg caccatggcg gcctcctcgg tgggtcggtg cccgagtatg
     1021 cccgagcccc cggtcggctc cgcggtgatc ggtggcgaaa ttggttcagg tccggggtcg
     1081 ggtgcgtcga caccccgaac gtgaacgcgt caccggttcg aacacggccg cgaggccagg
     1141 gcggcttcac gctgctcgag ctcctcgtcg tcgtcggcac cctcagcgtc atgtcgggcg
     1201 cagcggtcgt cggggtgggc tcgatgcgcg ccaacgctca acgggcggcc tgcgacgccg
     1261 atgccgacac gatcagcacc gccgaggagg ccgcgctgat ccagggcagc tacctgtccg
     1321 aggccgacct ggtggcgaag ggcttcctgg ccgacgaatc gacccgctgg gacgtcacgg
     1381 tgtcgggagc gtccttccaa cttgtgccga tcggcgactg cgtccccgac ggcacagagg
     1441 aggcggccgg gaccacaccg acaacggtgg acccgttggc gaagcagaag gcggagcagg
     1501 cagcccgcga caaggcgaag gccgaggcgt tggcgaagnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnga agcagaaggc tggccgcggg taggacccga ccgggcgcgg
     1681 ccggccgggt ccgggccgga tcaggccttg gtcaccgtca ggctgacgtc gccgacgccg
     1741 gccagcgcct tggacaccgg gcagttggcc ttggcgttct gggccagctc gtcgatcttc
     1801 tcggcgggca gatccacgtc gacggtggtc gacagagcga tgcgggtgat ggtcggaccc
     1861 tcgtcggtca cggcgatcga caccttggcg gtggtctcga tggaggtcgg cggggtctcg
     1921 ttcttggtca gcagcagcgc caggaactgt gagaagcagc cggcgtgagc cgcgccgatc
     1981 agctcctcgg gattggtccc cgcgccatcg gcgaacctcg acgccttggt gaaggtgccg
     2041 gtcagaccgg ttccggtgga gaccgcaccg gtcccctcgt cgatggtgcc ttcccagcgg
     2101 gcggtcgcag tcgctgccat gtgcggtgtc tcctcggtcg atcgttcagg gtcgatcgga
     2161 acctaccgct cacgtccggg agcgcccgag gcaccccgat ggtcggtccg gccgccggcc
     2221 cgctgatcag tcgacgtcgg tgcggcactt caggcggcag tgcgcccggc gcgggtcctt
     2281 ggtcaccagc tcctcgatgg tcagaccccc gaccgcatcc gcgatcccct cggccatccc
     2341 gaggtgcagc tggcagatgg tgtcggagtc ggtgagcacc gcggagggga acgggcactc
     2401 ctgggcggtg atctcgacgg cgtcgccgtg cacccgcacc cgcggcgaga agccctgtcg
     2461 gctcatctgg tcgaccagga ccgtcgtcgc gtcccgaccg tctgcgacct cgtgtcccag
     2521 gccagtcgcc acccgccgtc ccacctctcg gggtgtgtca ccgctgcgga tgatctcggt
     2581 gagcatcgtc gagagcatct cgtaggagcc gatcgctccc cagcggctct cggtcgtcgg
     2641 cgcgacggtg tactcgagcc ggggacgtcc ccgcccggac gggggtgcct gcgcctcggt
     2701 caccagaccc gcgtcgacca gcttggccag gtgctgacgg atggcgttgt ggttgaaccc
     2761 gaactgctcg gtgagctcgg ccacccccac cggccggtcg gcgtcgacga ggtagcggaa
     2821 cacggcgtga cgggtcgggc tgccgagcgc ccgggcctgc acctgcacgt cgtcgtccat
     2881 cggtccccag cgtagaagac gcaccggatc ggggcatgcg cagtgggtcg ggacgccccg
     2941 ccgggtcgtc aaccccgtcg ggtcctcaac ccccgacgga accgacgtcg gcccggaagg
     3001 cggcgagcag gtcgacctcg tcggagcggt cctcgccggc gaccaggctg atcaacgact
     3061 cggcctcctc catgcaggcg gggcagcggg ccaggtgcac ccgcagcggt tcgtccaggt
     3121 gggggtggtc gtgcaccagc gagtccacgt agaggtcgat ctgctcgaag cagtcgtcgc
     3181 aggacagcca gggctcgctg agcgccgtga ggctctcggc ctgggtcggg gtgatcttcg
     3241 tgccgtcggt cggtcccatc tcggtcacct cctgtgctcg tcgtggatgt agccctgctc
     3301 gcgcatctcg gagcggagct tcacccggat gtcgtgcagc gccttgtaga gggcgttgcg
     3361 gttggtcccc atccgctcgg ccaggacgtc gatcggcacc tcgtcgacga tgagggcgat
     3421 cgcgacccgg cgctggcggg ccgtgagcac ggtgtcgatg gcggtcgaca ccgcggcggc
     3481 caggtcccgg gcctcgacca ccgaggcgga cccgtcgtgg tcaccggtgg cgggctcggg
     3541 atgctcggtc aggtcgacgg ggcggtcccg ccacaacgcc cggcgggcct cggtcccggc
     3601 gtgcaggatg ccgaacttga agacccaggt gctgaaccgg ctgcggccct cgaagcgatc
     3661 gaggctgccc agcacggcgg tcgtggcctc atccgcggcg gcgtcgatga tctcctgggc
     3721 ccggacggct ccgagctggg accacacgtg cggcatccgg cgcacctggt gggcggcggc
     3781 tcggtgcacc gtgtcgtgca gtcgccgcac ggcggcgtga tgtccggggc cgtcaccccg
     3841 gagggcgtcg atccagtcgc agtcatcggt agggatcgtc ggcgcaccgg tcgcggtcgg
     3901 ggtgtcggaa ccggtcacgg ccaccatgct ggcacggacg ccgccgctga ccgagggggt
     3961 ccgccgccgg ggcgagcggc gcgaccccct cgagaccgac ccggcgtcgt ggggcgacgc
     4021 gcccccgggg ccggcggagg ccggccccgg agtgggcagg gtcacgacgt cgggtgcgag
     4081 gtccatggtc gtggcgtccc gcctcagcga cgcaccttgt tcgggaactg ggcggcgacg
     4141 ccctgggcga gggtgtcggc cagcatcatc atgtggtcgt aggcctcgtc gtagtgctcg
     4201 atcgccgagg cgtggtcgcc cttcagcagg tcggtggcgt acgccgtggt ctggtcgatg
     4261 tggcccttca tcatcggctc ggtcgccgag gtcggccagt gcttcgggtt ggcggccgag
     4321 agaaagtcgg cgatctcctt cgcgttggcg taccaggcgt cgagggcgga cttcagcgcg
     4381 gcctggtcgc cggactgggc ggcctgcagc accggcaccg cgccgttgat gtgagcggtc
     4441 agcagggcgg tgagagcgtc gcccgccttc gtgccgtaga agggcttgat ggcgttgccg
     4501 atgtcgctct ggttgcgcag cagccggtcg agggtcggct gcaccgcgga ggggttgtgg
     4561 aagaacgagt cgacggtcgc gtaggtccac tgcatgtggt ccgaccacag ctcgcgcatc
     4621 ccggaccgga gatcggagcg ggtgggtcgc tcggccgggg cggaggtctc ggtgccgtgg
     4681 ccgccgtggt tgccgacggg cggcgcggag accgggccgg cggagccggg gccgcaggcg
     4741 acgaggaacg tggcggcggt gacgacggtc gccaccgagg cgaagacccg gttgggtcgg
     4801 gaggtcgggg tcatcggggt gtccttcctt ctgcgcaccc gagggtgcgg tcgggttcgg
     4861 tcgggttcgt gggtcgagcc gtcccggtga cccgttgggt cacgtcggtt ctgtgtcgag
     4921 tcgggggcgg ggtcttacct ccggggtccg agaaatctgt gtcgcacccg ctcggaccga
     4981 cggcgtcaca ctcaccgcgt gacatgcacc gcgtgacatg caccgcgtgg catggcgacg
     5041 ctgtgtcggt atagttgtcg acgcggcgtc gacaactcgg cgtcgccgga aaggagttcc
     5101 agatgttcct cgcgttgcgg gacctccgac gaggagcccg acgcttcgcc ctcctggggg
     5161 tggtgatcgc actggtcgcg ctgctgtcga cggtgctgtc cgcgctcgcc accggtctgg
     5221 tcaccgacgg catctccggg ctgcgggccc tgccgttcga ccacctcgcc ctccaggacg
     5281 gatccgagtc caccttcagc cgctccaccc tcaagtccga cgcgctcgag aagttcgagg
     5341 gcctcgacgg cgtgaaggcc accccgctcg gcgcatcgtt cgtgaacgcc gcgtccaccg
     5401 acggcggccc caacctggac ctggcgctgt tcggcgtctc tcccgacagc ttcctggtcg
     5461 aacgccccga ggcccgcgcc gcgctcgacg gcccccccgg cctggtcctg gcccacgagc
     5521 tcgaggagca gggcgtcaag gtgggcgaca cctacacgct cggcggttcc gacgtggagc
     5581 tgccggtgct cgggttcacc ttcgccggca cctacggcca cgccccgatc gcgttcacct
     5641 cgctcgagac gtggcagaag gtccagttcg gtgacggagc cgacggccgg ttcgctgcgg
     5701 tcgcgctcac gggtggcacc gacccgcagc tcgagaaggc cgcgaccgcg acccacaccg
     5761 acgtcctgac caagaaggag gcgtacgccg gctcgccggg ctacaccgcc gagaccacga
     5821 cgatgtcgtt gatccggggc ttcctgctcg tgatctcggc gctcgtcatc ggcgccttct
     5881 tcaccgtgct caccgtgcag cgcacccgcc agatcggcct gctcaaggcg atgggcgcca
     5941 ccaacggcta catcctgcgc gacgggatcg gccagatggc gatcctggtc gtcctggcca
     6001 ccgccatcgg cgtggcgctc ggctgcacgc tcgtgttctt catgggccgg ggagacgcgc
     6061 cggtcgagct cagcgttccg gcggtgacca ccgtcgtcgt gtcgttggtg gtggcgggcg
     6121 tgctcggcag cctcgtcgcc ttcaagcgaa tcaccggagt ggaacccgcg atcgccctgg
     6181 gggtggaacc gtgaccgaac cagccgtcct cctcgaaggg atccgagtgg cgttccccga
     6241 cggccccgac gaacgggtcg tcctcgatga cctgaagctg tcggtggacc ccggtgagct
     6301 cgtcgtcatc tccggccagt ccggcgccgg caagtccacg ctgctcgcgg tcaccggcct
     6361 gctgcgccga gccgacgccg gcgaggtgac catcgccggc accgcgacct cggccatgtc
     6421 gagccgtcag cgcaccgcgg tgcgccggga ccacatcgcg ttcgtgtacc agtcggccaa
     6481 cctgctgccg tcgctgaccg cggtcgagca gctcgagctg gtcggacaca tccgcggcga
     6541 gcgctccgcc gatgtccgcc gccgcgcccg gggcctgctc gacgagctcg gcctggccga
     6601 tcgggcctct cagctgccgt cgcagctgtc gggcggcgaa cgccagcggg tcggcatcgc
     6661 ccgggccttc atggccgagc cgagcgtgct gatcgcggac gaacccaccg cgtcgctcga
     6721 tccgcagcgg gccgactcgg tcgccgagct gctcgccggc gcatcggcgc ggggcatcgc
     6781 caccatcgtg gtcgcccacg acaccgcggc gctgtcgcgg gccgaccggc acctccgcat
     6841 ggaggacggt cgactgctcg acgtgggagg ttgacgcgat gccgcggatc aaggccgaca
     6901 acatcgccga gcacgtcgca cagcagcgcg ccgcggtgct cgacgccgcg gtgcggctgt
     6961 tcgtcgagcg cgggttctcc gaggtgggcc tggccgacgt cgcggccgag gtcggcctgg
     7021 cccgcaactc gctgtaccgc tacgtgcccg acaagaccca cctgctcgtc gagtggtacc
     7081 gccggaccgt tccggccacg atcgcggact gggagcgggc caccgcgggc gacgacccgg
     7141 cgccggtgcg gctccaacgc tgggcccgct cctacctgga gtgggcgctg acaccggagc
     7201 accagctcgt cggcccgctc accgactccg tgtccggcct cgacgacgac acccgggccg
     7261 aagtggccga gctgcaccga tcgatgatgg cggtggtcgc ccgggtggtc gccgaggcgg
     7321 gcgtgtccgg ggaccacgtc gacggcgcgg ttgcgctgct cgccgggctg gtgctcggcg
     7381 cggcgcgggc cgagggaggg tccggagccg acgacgacct ccgccggcgg ctcgacaacg
     7441 cggtcgccgc cgtggtcgac accccgtagc ctcggcgcct gtcccgtccg ccgattcgag
     7501 tcggcgccgt ccgtcgagca ggcccccgtc ccgtgaccac gaccccctcc agcgctgcac
     7561 cgatcgccgc ggtcgacccg cgcaccgggc gaccgctcga cgtcgacgcc ctccagtgcc
     7621 ggacgttgcg ggtggtcgtg gcgtcgcaga tctgcggagg cgccggactg gccgccgggg
     7681 tcaccgtcgg agccctgctc gcccgggaga tgctcggcgg cgagggattc gccggtgtcc
     7741 ccaccgctct gttcaccctc ggctcggccc tcaccgcgtt cctggtcggg cgggtcaccc
     7801 aacgcgccgg ccgtcgtgtg ggtctggccg ccggcttcgg tgccggagcc atcggagcgg
     7861 tcggcatcgt cgccgcggcc gtgctctaca acgtcgtgct gctgctgccg tccctgctcg
     7921 tctacggcgc cgggaccgcc accaacctcc aggcccgcta cgccggcacc gacctcgccc
     7981 acgccgaccg acgcgccacc gcggtgagcg tcgccatggt gtcgaccacg atcggcgcgg
     8041 tcgcgggtcc caacctggtc gggccgctcg ggtccttcgc ccgttcggtc ggagcgccgg
     8101 cgttggcagg tccgttcgtg ctgggcgcgg tcgcctacgc gtcggcgggg acggtgctgt
     8161 tcctgttgct gcggcccgat ccgttcctgg tgtcacgctc gctggtcgac tccgagcgtc
     8221 ggtcggcgac cgacggctcc gccgcaaccg cgggtgccgc cgcggcggag caccccgaga
     8281 tgcccgcgtc cgtgccgtcg accgagcccg tccggcccga cgtgcgggtg ggcagcggtg
     8341 cctacctggg ggccgccgtc atggtcatca cccagatggc gatggtcgcg atcatgacga
     8401 tgacgccggt gcacatgcgg gcccacggcc acgacatcgg cgacgtcggc ctggtcatcg
     8461 ggttccacat cggtgccatg tacctgccgt cgttggtcac cggcgtgctg gtcgaccggt
     8521 tcggccgcat caccatggcg gtcgcggcgg cggtgacgct gctcgcggcg ggcgtgaccg
     8581 cggcgacagc gccgggggag tcgttgccgg cgctcgtggc ggcgctggtc ctgctgggtc
     8641 tcggatggaa cttcggcttg atatcgggga cggcgctcgt cgtggacgcg acctcccccg
     8701 aggaccgacc ccgggtgcag gggacgatcg acgtcggcgt cgccctggcc ggcgccggtg
     8761 gtggcgtgct gtcgggtgtg atcgtcgccg gatcgagcta cgcgaccctg tcgctgctcg
     8821 ggggagtgct gtcgctggcg gtgatcccgg tcctgtggtg gacccggcgc atggaggccc
     8881 ggcaccgggc tcagcgcggg ctgacggttc cgggttgaag gttccggttg acggttccga
     8941 cgaccacgca cgacaccgaa acgtcgccga tgccctcaac cgccaccgcc ggaccatcga
     9001 gaacagaccg cccccaggca tcgcctcttg accctacccc gagggccgga tgatcggttt
     9061 cgggttccgc cgcttcgcca actccctacc tgtcagtatg agtgtcgctc agtggagtga
     9121 ccatattcga tgagggcg
//
LOCUS       DAOQOD010000125         9112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2798_length_9112_cov_8.878989, whole genome shotgun sequence.
ACCESSION   DAOQOD010000125 DAOQOD010000000
VERSION     DAOQOD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_9112_cov_8.878989"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLZ72_11665"
     CDS             complement(<1..805)
                     /locus_tag="PLZ72_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA36999.1"
                     /translation="MVATTRLEPLNAADAELFAAARLRAARSQPYLATALFAMVPVVR
                     PGFGTFAVDRYWRVYLDMDQAREWGVAATAAVLVHEAHHLVRGHHERARRVGATGERH
                     RDWNLAADAAINDDLVDDGLDLPDPVLPHHLGCRRGGFEEVYYRALRDPHHHRPERPR
                     RPVVDLSDDPDDPDGDDCDADDPEPDPGCGSGSGGAALPVEIDDEPTDPTEGLDDVDA
                     DAVRRAVAHDIASAHAAGGTVPPGLHRWATALLTPQVPWRTLLRSAVRRD"
     gene            complement(818..1891)
                     /locus_tag="PLZ72_11670"
     CDS             complement(818..1891)
                     /locus_tag="PLZ72_11670"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA37000.1"
                     /translation="MRNALAVAVQAGQPVLLWGGPGEGKSRLVESIAEQLGRPCEVVV
                     GSVREASDFAGLPVVEHGSVSFAPPRWATRCVERPDTVVFLDELTTAAPSVQAAMLRV
                     VLEREVGDLRLPPTVAFVAAANPPELSAGGDDLSAPLANRFCHLDWEADPAGWATGML
                     RGWAPRHVPVVPAAFAAHRDRWRAIVVGFIRSRPATLRQVPTDVVGQGRAWPSPRTWD
                     RAHVVAAAADAAGAGRDVIRLLVAGLLGQGPAIELLRFADELDLPDPELLLAEPDLLP
                     TDRRVDLLLAALAGVTAAVAGDLSLDRWEAAWRVLSVACDAGRADVAAMSATALVELR
                     LDDWPAPRAAAAFAPMLRAAELV"
     gene            2045..3067
                     /locus_tag="PLZ72_11675"
     CDS             2045..3067
                     /locus_tag="PLZ72_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HNA37001.1"
                     /translation="MDPAHAGSAPPLDDPRRTRRRARLVEELADTGFVLPGPASLAEA
                     ALSELDYAMRPRVHERRVPSYGAIIAPTGPREGWQTSTRLTVTARPFPHGGLAGARMF
                     ADGLSSWVIRGVDDLLGPDASDDELVVFDRPAGSERDVVVLAESTGGIVVQRHPSGVV
                     RVAGEFGVLRWDGVAWQQQPPVGEWVETFAECSGPEQREVVETLLEIAVHDLGARGIG
                     AILVYHRESAGPGLGDGPHHFETRRPVPPALSVLNPADLAPLVHVLAQIDGAAVFDRD
                     GVLRELGVRLRPRPQTESEVEGFGGMRHTTARRYSVDEPDAVVIVVSEDGPVTVMRRG
                     SIHLGG"
     gene            complement(3074..3724)
                     /locus_tag="PLZ72_11680"
     CDS             complement(3074..3724)
                     /locus_tag="PLZ72_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37002.1"
                     /translation="MTTAMSRRGRPRISDEERAERRTELTGRAMAAVREGGGDQSLDE
                     IAAHIGVSKPVLYDTFGSRTGLADALALALAERTEAFVVERFGPALPDGTYDVSVERV
                     IELIVTALVFLVDQEPELYTFITSAMRGDRRGMLDNSLVAVVRERIRPVTELATPGLG
                     PAEHSVLTDGMYGLVLATLESWRDSDEVDRDVVVGMLTALITNGMRAIADGVPPSG"
     gene            complement(3721..4209)
                     /locus_tag="PLZ72_11685"
     CDS             complement(3721..4209)
                     /locus_tag="PLZ72_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA37003.1"
                     /translation="MSFSRDEIQDAFDRFAAAADHGGRTGDWRPWVECFVPDVHYVEH
                     LYGEFDGRDAVLEWITSTMGAWPFDHMQLFPWDWYTIDAEQGWVVGQVQNRFVDPGDG
                     SVHQAPNWTRLVYAGDGLFASEEDVYNPSHFGPMVAAWLDAWRTQHPDLPHGPGPRET
                     DR"
     gene            complement(4256..4615)
                     /locus_tag="PLZ72_11690"
     CDS             complement(4256..4615)
                     /locus_tag="PLZ72_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37004.1"
                     /translation="MTGRSRWAQLGPMGPELSLAQTVDDLSARGWDAEFAAAEGGLLT
                     CGLCGDTLSPTEVHIDSVYRFEGQSDPDDQAAVFALTCHCGCRGIYVIAYGPSMSGAD
                     ADVVSHLSRRTASEPEI"
     gene            4684..5361
                     /locus_tag="PLZ72_11695"
     CDS             4684..5361
                     /locus_tag="PLZ72_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNA37005.1"
                     /translation="MTTAGRPKILEALDAYGPRPVWRGRVHFLAALVAAPAALVLFLV
                     APGPMAKVAVTIYAVSLVGLFAVSASYHRLAHTETAVKWFRRADHSMIFVLIAGTYTP
                     LCLLALPPAWGIPLLVAVWVAAIGGVITKMTMLGRGPGSAGSWLYIVIGWAAVVAVPA
                     LVTNLDALQLVLLAVGGLLYTVGAIVLGKRWPDPVPHVFGYHEVWHSMTVAAGSCHFA
                     VVALVLR"
     gene            5379..6686
                     /locus_tag="PLZ72_11700"
     CDS             5379..6686
                     /locus_tag="PLZ72_11700"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNA37006.1"
                     /translation="MDHTGVTASHRAQPQSKPTVEVVAYRATWPADFASERTALAAAT
                     GGQALTIEHIGSTAVPGMSSKPTIDILMVLERADDVADHLRALADVGYELQPGAFPDR
                     AGHLFMHKVVEGRRLAHLHILDADSVEIDDYRRFRDALRADPDLAARYQALKVDLAER
                     FGDDRARYVAEKERWADAVVGSLRAEGTGADALYDRIGVGYAGQRVSEPTWVSQIGAA
                     LGASRSVLNVGAGSGNYEPVDRRVVALEPSSTMLAQRDGRHPAVQGVAEHLPFPDGCF
                     DAALGTFTVHHWTDPARGLAELARVADRQVLVVFEPLVAHGFWLLDYFPEVLTSDVEL
                     NAPTPDDVGRHLDVVDVQTMWIPAECRDGVAAAYWRRPEAYLDPVVQRSMSLLALLPE
                     RVVARGAAELAADLADGTWLERHGHLLEQDRADYGYRLVIAGS"
     gene            complement(6735..7730)
                     /gene="egtD"
                     /locus_tag="PLZ72_11705"
     CDS             complement(6735..7730)
                     /gene="egtD"
                     /locus_tag="PLZ72_11705"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNA37007.1"
                     /translation="MTPITDTTTTIRVRVHLDPADWSRHLAEETRRGLTDDPPWTPPV
                     WFYDEVGSHLFDRITRLDEYYPTEAERSILAARSAEIARLTGARTLVELGSGTSDKTR
                     LLLDALWAAGTLRTFSPVDVSEEVLRTAATAIAEGYPGIAVDAVVGDFGRHLDRIPTG
                     GRTLLAFLGSTIGNLDPDQRRAFLASVTDALAPGDWFLLGTDLVKDEARLLAAYDDAA
                     GVTAAFNRNALVVMNRELGADFDPDAYSHRSHWDPVEHRIEMHLVADRDQRVTVRGLD
                     DLVVDLGAGEHLRTEISTKFTAEGVEHELLAAGLEPVAAWTDPAGDVRLTLARRP"
     gene            complement(7744..9090)
                     /gene="egtB"
                     /locus_tag="PLZ72_11710"
     CDS             complement(7744..9090)
                     /gene="egtB"
                     /locus_tag="PLZ72_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HNA37008.1"
                     /translation="MPVTTATGRPAPSRGADPAAFCTVRARTEALAAPLGAEDQVVQS
                     MPDCSPTKWHRAHTTWFFEEFVLSRHVAGYRPVDDSYRYLFNSYYEAVGERQPRPRRG
                     LITRPTVEEVAAYRRAIDRSVLDLLGRPGDPAAPLEPEVAALVELGLHHEEQHQELLL
                     MDAKHLLFQNPQHPAYRSDAPDADDDADADTSPTRRGDTAPTGVQGWIPHEGGPVEIG
                     VAAGDPTFSFDNEGPRHTVQLQPFAIADRLVTNGEWQGFIDDGGYLRPELWLSDGWAT
                     VNEQGWRAPSYWFGLDDPDGPRVFTLAGPEAVRAADPVVHVSLYEADAFARWAGARLP
                     TEAEWEVVARDRWGADTDALATAPHPRPGADGSDPAQFRGAVWQWTASAYLPFPGFQP
                     AAGAVGEYNGKFMVNQHVLRGGSCATPPGHARPTYRNFFPPAARWPFTGLRLATDA"
BASE COUNT         1154 a         3217 c         3470 g         1271 t
ORIGIN      
        1 ggtcgcggcg caccgctgag cggagcaggg tccgccacgg gacctgcggc gtcaacagcg
       61 cggtggccca ccggtgcagg ccgggcggga cggtcccgcc ggcggcgtgg gcggaggcga
      121 tgtcgtgggc cacggcgcgg cgcaccgcgt cggcgtcgac gtcgtcgagg ccctcggtcg
      181 ggtcggtggg ttcgtcgtcg atctcgacgg gcagggccgc gccgccggag ccggacccgc
      241 accccgggtc cggctccggg tcgtccgcgt cgcagtcgtc gccgtcgggg tcgtccggat
      301 cgtcggagag gtcgacgacg gggcgacggg gccgctcggg gcggtggtgg tgcgggtcgc
      361 gcagcgcccg gtagtagacc tcctcgaacc cgccccggcg gcacccgagg tggtgcggca
      421 ggaccgggtc gggcaggtcg agcccgtcgt cgacgaggtc gtcgttgatc gcggcgtcag
      481 cggccaggtt ccagtcgcgg tgccgctcgc cggtcgcccc cacgcgtcgg gcccgctcgt
      541 ggtggccgcg gaccaggtgg tgggcctcgt gcaccagcac ggcggccgtc gccgccaccc
      601 cccactcgcg ggcctggtcc atgtcgaggt agacccgcca gtaccggtcg acggcgaagg
      661 tcccgaaccc cggcctgacg acggggacca tggcgaacag ggcggtcgcg aggtagggct
      721 ggctccgcgc cgcacggagg cgggcggcag cgaacagctc ggcgtccgcg gcgttcaggg
      781 gctccagtcg ggtggtggcg accacggcgg aaccggctca gaccagttcg gcggcgcgca
      841 gcatcggtgc gaacgcggcg gcggcgcggg gagcgggcca gtcgtcgagg cggagctcga
      901 cgagcgcggt ggccgacatg gccgcgacgt cggcgcggcc ggcgtcacac gcgaccgaca
      961 gaacccgcca tgccgcctcc catcggtcga ggctcaggtc cccggcgacc gcggcggtga
     1021 ccccggccag cgcggcgagc aggaggtcga cccttctgtc ggtgggcagg aggtcgggtt
     1081 cggccagcag cagctcgggg tcggggaggt cgagctcatc cgcgaagcgg agcagctcga
     1141 tggccgggcc ctggccgagc agcccggcca cgagcaggcg gatcacgtct cgtcccgctc
     1201 ccgccgcgtc ggccgccgcg gcgacgacgt gggcccggtc ccacgtgcgc ggtgacggcc
     1261 acgcccgacc ctggccgacc acgtcggtgg ggacctggcg cagcgtcgcc ggacgggacc
     1321 ggatgaagcc gaccacgatc gcccgccacc ggtcgcggtg cgcggcgaac gcggcgggca
     1381 ccaccggcac gtgtcgggga gcccatcccc gcagcatccc ggtcgcccaa ccggcggggt
     1441 cggcctccca gtccaggtgg cagaaccggt tggccaacgg cgccgacagg tcgtcgcctc
     1501 cggcggacag ctcgggcggg ttggccgccg cgacgaacgc gacggtcgga ggcaggcgca
     1561 ggtcgccgac ctcgcgctcg agcaccacgc gaagcatggc cgcctgcacc gacggggccg
     1621 cggtggtcaa ctcgtcgagg aacacgacgg tgtcgggccg ttccacgcaa cgggtggccc
     1681 accgcggtgg ggcgaagctc accgacccgt gctcgacgac cggcagcccg gcgaagtcgg
     1741 atgcttcgcg gacgctgccg acgacgacct cgcacgggcg gccgagctgc tcggcgatcg
     1801 attcgaccag gcgtgacttg ccctcgccgg gcccgcccca cagcaggacc ggttgaccgg
     1861 cctggaccgc caccgccagt gcgttgcgca cggcgtcagc gctcccacgc agcggttgat
     1921 cggagctgtt cccggccatc gacaccctcc cagaacctgt gttcgacccg agctcctatt
     1981 acgaacacac gttcgcgccg ggcgcaagcg ggttccgcgg gatcgggcgc ggtaccttcc
     2041 cggcgtggac cctgcgcacg ccggttcggc ccctccgctc gacgatccgc gccgcacccg
     2101 tcggcgcgct cgtctggtcg aggagctcgc cgacaccggc ttcgtgctgc ccggacccgc
     2161 gtcgttggcc gaggcggccc tcagcgagct cgactacgca atgcggccac gggtccatga
     2221 gcgtcgcgtc ccgagctacg gggcgatcat cgccccgacc ggaccgcggg agggatggca
     2281 gacatcgacc cggctcaccg tcaccgcccg cccgttcccc cacggcgggc tggccggcgc
     2341 ccgcatgttc gccgacggcc tgtccagctg ggtgatccgc ggggtggacg acctcctggg
     2401 gcccgacgcg tcggacgacg agctggtcgt gttcgaccgg cccgcgggtt ccgagcgcga
     2461 cgtcgtcgtg ctggccgagt cgaccggcgg gatcgtggtg caacggcacc cgtcgggcgt
     2521 ggtgcgggtg gccggcgagt tcggggtcct gcgctgggac ggggtcgcct ggcagcagca
     2581 gccgccggtg ggcgagtggg tggagacctt cgccgagtgc tccgggcccg agcagcgcga
     2641 ggtggtcgag acgctgctcg agatcgccgt gcacgacctg ggcgcccggg gcatcggggc
     2701 gatcctggtg taccaccgcg agagcgccgg tccaggtctg ggcgacgggc cgcaccattt
     2761 cgagacccgc cggccggtcc ctcccgcact gtcggtcctc aacccggcgg acctggcgcc
     2821 gctggtgcac gttctggccc agatcgacgg cgccgcggtg ttcgaccgcg acggggtcct
     2881 gcgcgagctc ggcgtgcggc tgcggccccg cccccagacc gagtccgagg tcgagggctt
     2941 cggtgggatg cgccacacca ccgcccggcg ctacagcgtc gacgagcccg acgcggtggt
     3001 gatcgtggtc agcgaggacg ggccggtcac cgtcatgcgc cggggatcga tccacctcgg
     3061 cggctgagcc ggctcagccc gagggcggca ccccgtccgc gatcgcccgc atcccgttgg
     3121 tgatcaacgc ggtcagcatg ccgacgacga cgtctcggtc gacttcgtcg gagtcccgcc
     3181 aggactcgag ggtggccagc accaggccgt acatgccgtc ggtcagcacc gagtgctcgg
     3241 ccggacccag accgggggtg gccaactcgg tcaccggtcg gatccgctcg cggaccaccg
     3301 cgaccagcga gttgtcgagc atgccgcgcc ggtcgccgcg catcgccgag gtgatgaacg
     3361 tgtacagctc cggttcctgg tcgaccagga acaccagggc ggtgacgatc agctcgatga
     3421 cccgctcgac cgacacgtcg taggtgccgt cgggcagcgc gggtccgaag cgctcgacca
     3481 cgaacgcctc ggtgcgttcg gccagggcca gggcgagggc gtcggccagg ccggtgcggc
     3541 tgccgaaggt gtcgtagagc accggcttgg acaccccgat gtgcgcagcg atctcgtcga
     3601 gggactggtc gccgccgccc tcgcggaccg cggccatcgc ccgcccggtc aactcggtgc
     3661 ggcgctcggc gcgctcctcg tcgctgatgc ggggccgccc ccggcggctc atggcggtgg
     3721 tcaccggtcc gtctcccgcg ggcccggacc gtgtggcagg tcggggtgct gcgtccgcca
     3781 cgcgtcgagc caggccgcga ccatcggtcc gaagtgggac gggttgtaga cgtcctcctc
     3841 cgacgcgaac aggccgtcgc cggcgtacac gagccgcgtc cagttggggg cctggtggac
     3901 cgagccgtca cccgggtcga cgaagcggtt ctgcacctgg ccgaccaccc aaccctgctc
     3961 ggcgtcgatc gtgtaccagt cccaggggaa cagctgcatg tggtcgaacg gccaggcccc
     4021 catggtggag gtgatccact cgagcaccgc gtcgcgaccg tcgaactcgc cgtagaggtg
     4081 ctccacgtag tggacgtcgg gcacgaagca ctcgacccac ggcctccagt cgccggtgcg
     4141 tccgccgtgg tcggccgcgg cggcgaaccg atcgaaggcg tcctggatct cgtcgcggct
     4201 gaagctcatc gatgtccccc gacggtccgc ggcacgggcc cgcccctctg tgcggtcaga
     4261 tctccggttc ggacgccgtg cgacgcgaca ggtgcgacac cacgtccgcg tccgctccgc
     4321 tcatcgacgg accgtaggcg atgacgtaga tcccccgaca gccgcagtgg caggtgaggg
     4381 cgaacacggc ggcctggtcg tcggggtcgc tctggccctc gaagcggtac acgctgtcga
     4441 tgtggacctc ggtcggcgac agggtgtcgc cgcacagccc gcaggtcagc agcccgccct
     4501 cggcggcggc gaactcggcg tcccagcccc tcgcggacag gtcatcgacg gtctgcgcca
     4561 gcgacagctc gggtcccatg ggtccgagct gagcccagcg ggagcgtccc gtcaaggacg
     4621 atccctcatc ccgctcagcg tcctgccctt ccatcggagg ccgtcgcgtg gtccactggt
     4681 ccggtgacca cggcgggacg accgaagatc ctcgaagcgc tcgacgccta cgggccccgt
     4741 ccggtgtggc ggggtcgggt gcacttcctg gccgcgctgg tggccgcgcc ggctgctctg
     4801 gtgctgttcc tcgtcgctcc cggtcccatg gccaaggtcg cggtgacgat ctacgccgtg
     4861 tcgctggtgg ggctcttcgc cgtgtcggcc agctaccacc gcctggccca caccgagacg
     4921 gcggtcaagt ggttccgccg cgctgaccac tcgatgatct tcgtgctgat cgccggcacc
     4981 tacaccccgc tgtgcctgct ggcgctgccg ccggcgtggg ggatcccgct gctggtcgcg
     5041 gtgtgggtgg ccgccatcgg cggcgtcatc accaagatga ccatgctcgg tcggggaccg
     5101 ggcagcgccg ggtcgtggct gtacatcgtg atcggctggg ccgcggtggt cgcggtgccg
     5161 gccctggtca ccaacctcga cgcgctccag ctcgtgctgc tggcggtcgg cgggctgctc
     5221 tacaccgtcg gggcgatcgt gctgggcaaa cgctggccgg accccgtgcc gcacgtgttc
     5281 ggctaccacg aggtgtggca ctcgatgacc gtcgcggcgg gctcgtgcca cttcgcggtg
     5341 gtcgccctcg tgctgcgctg agcgcaccgc catccgggat ggaccacacg ggcgtcaccg
     5401 cgtcccaccg ggcgcagccg cagtcgaagc cgaccgtcga ggtggtggcc taccgggcga
     5461 cctggccggc ggacttcgcg tccgaacgta cggcgctggc cgcagcgacc ggcgggcaag
     5521 ccctgacgat cgagcacatc ggcagcaccg cggttccggg gatgtcgtcc aagccgacga
     5581 tcgacatcct gatggtgctc gagcgggccg acgacgtggc ggaccacctg cgagcgctgg
     5641 ccgacgtcgg ctacgagctg caacccgggg cgttccccga ccgtgccggc cacctgttca
     5701 tgcacaaggt cgtcgagggc agacggctcg ctcacctcca catcctcgac gcggactcgg
     5761 tcgagatcga tgactaccgc cggttccgcg atgcgctgcg agccgatccg gaccttgcgg
     5821 cccgctacca ggcgctgaag gtcgacctgg ccgagcggtt cggcgacgac cgggcgaggt
     5881 acgtggccga gaaggagcgc tgggccgacg cggtggtggg ttcgctgcgc gccgaaggga
     5941 ccggggccga cgcgttgtac gaccggatcg gggtcggcta cgcgggccag cgggtgtcgg
     6001 agccgacgtg ggtgtcgcag atcggcgccg cgttgggggc gtcgcgttcg gtgctcaacg
     6061 tgggggccgg gtcagggaac tacgagccgg tcgaccggag ggtcgtcgcg ctcgagccgt
     6121 cgtcgaccat gctcgcccag cgcgacgggc gccacccggc ggtgcagggg gtcgccgagc
     6181 acctgccctt cccggacggg tgcttcgacg ccgcgctcgg cacgttcacc gtgcaccact
     6241 ggacggaccc ggcccgcggg ctggccgagc tcgcccgggt ggccgaccgc caggtgctcg
     6301 tcgtgttcga gccgctggtc gcccacgggt tctggctcct cgactacttc cccgaggtgc
     6361 tgacctccga tgtggagctc aacgctccga cccccgacga cgtcgggcgg cacctcgacg
     6421 tcgtcgacgt gcagacgatg tggatccctg ccgagtgccg tgacggagtg gcggccgcgt
     6481 actggcgacg ccccgaggcc tacctcgacc ccgtggtgca gcgctcgatg tcgctgttgg
     6541 cgctgctgcc cgagcgcgtc gtcgcccgtg gtgcggccga gttggcggcg gacctggccg
     6601 acggcacctg gctcgagcgc cacggccacc tgctggaaca ggaccgcgcc gactacggct
     6661 accgcctggt catcgccggg agctgacgcc cgggcgtcgg tgggtcgccc ggtgatggtg
     6721 cggcggaccg gcgttcaggg ccgtcgggcg agggtgagcc tgacgtcgcc ggccgggtcg
     6781 gtccacgccg ccaccggctc caggccggcg gcgagcaact cgtgctcgac gccctcggcg
     6841 gtgaacttgg tgctgatctc ggtgcgcagg tgctccccgg cacccagatc gacgaccagg
     6901 tcgtcgagcc cgcgcaccgt gacccgctgg tcccggtcgg ccaccaggtg catctcgatg
     6961 cggtgctcga cggggtccca gtgcgagcgg tgcgagtagg cgtcggggtc gaagtccgcg
     7021 ccgagctcgc ggttcatgac cacgagcgcg ttccggttga acgccgcggt gaccccggcg
     7081 gcgtcgtcgt aggcggcgag cagcctcgcc tcgtccttga ccaggtccgt tccgagcagg
     7141 aaccagtcgc cgggggcgag ggcgtcggtg accgacgcca ggaaggcccg acgttggtcc
     7201 gggtcgaggt tgccgatcgt cgagcccagg aaggcgagca gggtgcgccc gccggtgggg
     7261 atccggtcca ggtggcggcc gaagtcgccg accacggcgt cgacggcgat gcccgggtac
     7321 ccctcggcga tcgcggtggc cgcggttcgc aggacctcct cgctgacgtc gacgggcgag
     7381 aacgtgcgca gcgtcccggc cgcccacagg gcgtcgagca gcagacgggt cttgtcggag
     7441 gtgcccgatc cgagctcgac cagggtgcgg gctccggtga ggcgggcgat ctcggcggag
     7501 cgggcggcca ggatcgagcg ctcggcctcg gtcgggtagt actcgtccag ccgggtgatg
     7561 cggtcgaaca gatgcgagcc gacctcgtcg tagaaccaga ccggcggcgt ccacggtggg
     7621 tcgtcggtca gtccgcggcg ggtctcctcg gccaggtgcc gggaccagtc cgcggggtcg
     7681 aggtgcaccc ggacccggat cgtcgtggtg gtgtcggtga tgggggtcat cggcgtgggg
     7741 acctcaggcg tcggtggcca ggcgcaggcc ggtgaacggc caccgcgccg ccggcgggaa
     7801 gaagttgcgg taggtcggtc gggcgtggcc gggtggggtg gcgcacgatc cgccccggag
     7861 cacgtgctgg ttgaccatga acttcccgtt gtactcgccg acggcgcccg cggcgggctg
     7921 gaacccgggg aacgggaggt acgcgctggc ggtccactgc cacaccgcgc cgcggaactg
     7981 cgccgggtcg gacccgtcgg cgccgggtcg agggtgcgga gcggttgcca gcgcgtcggt
     8041 gtcggcgccc cagcggtcgc gggcgacgac ctcccactcg gcctcggtgg gcagtcgtgc
     8101 cccggcccag cgggcgaagg cgtcggcctc gtacaggctg acgtggacga cggggtcggc
     8161 ggcgcgcacc gcttcgggcc cggcgagggt gaacacccgg ggcccgtcgg ggtcgtcgag
     8221 gccgaaccag tacgacggtg cccgccagcc ctgctcgttc accgtggccc agccgtcgct
     8281 caaccacagc tcggggcgca ggtagccgcc gtcgtcgatg aagccctgcc actcgccgtt
     8341 ggtgaccagt cggtccgcga tcgcgaacgg ctgcagctgc accgtgtggc ggggcccctc
     8401 attgtcgaac gagaaggtcg ggtcgcccgc tgccaccccg atctcgaccg gcccgccctc
     8461 gtgggggatc cagccctgaa cgccggtggg tgcggtgtcg ccgcgccggg tcggcgacgt
     8521 gtcggcatcg gcgtcgtcgt cggcgtccgg cgcgtcggag cggtacgcgg ggtgctgcgg
     8581 gttctggaac agcaggtgct tggcgtccat gagcagcagc tcctggtgct gctcctcgtg
     8641 gtgcagtccg agctcgacca gggcggcgac ctcgggctcg agcggtgctg cggggtcgcc
     8701 ggggcggccg agcaggtcga ggaccgagcg gtcgatggcc cggcggtacg cggcgacctc
     8761 ttcgacggtg ggtcgggtga tcagcccgcg tcggggtcgg ggctgacgct cgccgaccgc
     8821 ctcgtagtag ctgttgaaca ggtagcggta cgagtcgtcg accggccggt agccggcgac
     8881 gtggcggctc aggacgaact cctcgaagaa ccacgtcgtg tgtgcccggt gccacttggt
     8941 cggtgagcag tccggcatcg actgcaccac ctggtcctcg gctccgagcg gggcggccag
     9001 cgcctcggtg cgggcgcgga cggtgcagaa cgcggccggg tccgcgcccc ggctcggcgc
     9061 cgggcggccg gtggcggttg tgacgggcat ggcgactccc tcccggatcg at
//
LOCUS       DAOQOD010000126         9088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2810_length_9088_cov_10.200487, whole genome shotgun sequence.
ACCESSION   DAOQOD010000126 DAOQOD010000000
VERSION     DAOQOD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_9088_cov_10.200487"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLZ72_11715"
     CDS             <1..684
                     /locus_tag="PLZ72_11715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37009.1"
                     /translation="GGPLAGSLAETAALAAATGSTAVGVVADLSDPSDRARVVPEAVD
                     ALGGPIDVLVNNAAAAIYGPLGELTLKRRRLMFEVNVHAPVDLAQGVVPAMAEAGEGW
                     IVNVSSRTSRPWPGPPFELGVTGTTTGAYGASKAALDRMTNALAAELAPTGIRVNAVA
                     PRAAVMTEGAEALVGDRIGEDRIESLEHMVEAVVALCCGGPELTGRNATSHEIIDELG
                     LAVRSLEGT"
     gene            681..2234
                     /locus_tag="PLZ72_11720"
     CDS             681..2234
                     /locus_tag="PLZ72_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNA37010.1"
                     /translation="MTAVRIIYFDIDTLRADHLGCYGYHRDTSPNIDALAAEGIRFES
                     VHASDVPCLPSRTALATGTFGTRNGVVNHGGVAADLRVEGADRQFVSSTMRSSWAWRL
                     YTAGFHTASMSSFPFRHSASWWNHGYMEAANLMRGMGVERSPAVTEHVVDWLRRRGRA
                     DRWFLHVHLWDPHTPYNTPDDYGNPFEGDPIPAWHTEEVRAANWERPGPHSAQEPWGF
                     TPDEWGPPPPRQPWDLSSMDDVKAAFDGYDVGIRYADDAVGAVVSELADLGVLDETAV
                     LVSSDHGEQFGELGVYCDHQGADQATTHIPAVLRWPGLEPRVQPGLHYHLDLAATVVE
                     LAGAVVPTDVWDGESLAPALHAGTTAGRDHLVLTQGAWTAQRGVRTGDHLYLRTFHDG
                     YHGWDDEMLFDVAADPHEMTDLIDEPSAAAAADRARSVLDDWTATQLARSGAEDPIDT
                     VLAEGGPYHTRGHLPAYLQRLRDTGRSGWADLLAERHAAEADPGRWADFERTRRRLAY
                     DLMGIAEPS"
     gene            complement(2209..3144)
                     /locus_tag="PLZ72_11725"
     CDS             complement(2209..3144)
                     /locus_tag="PLZ72_11725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNA37011.1"
                     /translation="MDELPSLSERQLGYVVAAVEEGSFTEAAIECSVSQPTLSASIGR
                     LERDLGIALFDRIGRRIVPTDACLAMLPAARDLLRAAADARRAVSSVADGLSGHLDLA
                     VQPTVVGTVVGLVAEFRRDHPGVSVHLRSPGDGSVGALVASGVADLGIGDVAEPSLRW
                     RALWDEPYVWVEPDLDGPRPTAGSGQRRRPRPRPVRADRLGGAVLVVPPDGSPTRQVI
                     DAWFAAASSSPQRALEVDHREAILALVAAGAGSTIVPASMAAGSDTAAVRVRPLDPPI
                     ERTIGIVHRDRPLSPLAARFVELAAERFNSARRSP"
     gene            3215..3964
                     /locus_tag="PLZ72_11730"
     CDS             3215..3964
                     /locus_tag="PLZ72_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA37012.1"
                     /translation="MLWLEIALIGLGIGFLGGLFGKGGSAVATPLLYLVGVPAIAAVA
                     SPLPATIPGTLVAAGAYRRAGMIELRTVGWSLATGLPATVLGALATNWIDGSALVTAT
                     EIILIGIGLRLVISRHGLGADPTGDESPSIERWRLLLVGGLVGLIGGLLANSGGFLLA
                     PLYLAVAHLRIKPALAASLAVSAVLAVPGTVVHGFLGHIDWAVAATLAVTSVPASRLG
                     ATVAIRTASDRLERLYGAALVVLGAGLLLVS"
     gene            complement(3980..5476)
                     /locus_tag="PLZ72_11735"
     CDS             complement(3980..5476)
                     /locus_tag="PLZ72_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA37013.1"
                     /translation="MSSTARLSPLRRGRFVAVVAAVSALAVLAGACGARGGEHITDGA
                     EMNGTPQGGAAATAPADDGAAVDSPDFGTVKAPCGKGDAKVADGEQQGGADTIQIGVP
                     NDRGATIRPGLQKPIWDASVAFAAWCNAQGGIGGLKIELIDMDGKLFEVEAAMQKACG
                     NAFALVGGGNVQDDLQFSGKDGSDFHRCKLIDIPAFAVSSAKSMSNGQVQPIPNPTNT
                     ISNTWFRDFFALYPDQAAKWAPVWGNLPSLKIVKTKQEAIIPDVKGMSQVGDFNYPPI
                     GTTDWTPLALSVIDSGATSLSWTGESPDIAKLLEKLKDQGWKGRALLETNQYDKLFIE
                     SGSPKALDGSLVRMAPHMLEEADKWPAVRKYLDLIDQYVDGGEVAVLGMQSMSAWLLF
                     VTAANKCAADNGGEISRNCIMETAAATSDWTGGGLHAPQDPDEGPTMKASTCSMLVMV
                     KDGKFVRRYPEIGGEGDDGDGFHCPADGLTKVTADVGEGNVDPTRPPY"
     gene            5664..6542
                     /locus_tag="PLZ72_11740"
     CDS             5664..6542
                     /locus_tag="PLZ72_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA37014.1"
                     /translation="MQSDVDFLGMAPGPGADRFRFTICGHLARLDGLLYGGTAIAASM
                     AAAEAVTGRSSLWTTTQFVATAGPGTEVTVTVEELAAGRRAAQVRVTGTDPTGRVLFA
                     SLGATGEADMERPRGVFLDPPRVADPDDSPPWGGPIATIVRLIADAEESSGRPPGVGF
                     MSVVELLEPEVVSHPDPGPGRLTYWARRVDGGPMTPAIVAYIADLVPLSVSTAMGVVE
                     VGTSLDNTIRVDPSVQTDWVLLDLRPHASIGGCCHGEVHAWAPDGRLLATASQTALLR
                     PLDPALVAAFRAGPSD"
     gene            6616..7239
                     /locus_tag="PLZ72_11745"
     CDS             6616..7239
                     /locus_tag="PLZ72_11745"
                     /inference="COORDINATES: protein motif:HMM:NF037005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37015.1"
                     /translation="MEPELSVRHRPVQARGRERFELILRTGRDQLARVGVDGFTFDGL
                     ADAAGIPIGSIYQFFPNKHALICELSAQDTDAIVAALGRAAEQFPSDDWQTDVDRLID
                     HLADLWRSEPGRRHVWLAMQSTALTRSLAAEQTTRIAEQIVPLVAWLMPEGSRRRART
                     VAAVVVQMCYSLLHFSVHDDRPHPATVRELKRALRSYLRAVALDAQG"
     gene            complement(7258..8916)
                     /locus_tag="PLZ72_11750"
     CDS             complement(7258..8916)
                     /locus_tag="PLZ72_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNA37016.1"
                     /translation="MLDAARHATPERRAELASLAASSGRFAVNAGNHDVARHLLQAAL
                     ELSEHRGVERARLLLNLARATAGSGDLVGARTLLDEVATLAETAGDVDLLVDAAIRST
                     FPADWRAGDRHSAALLDLAERLDDGARRGAILACRALVEMRIPASSDPDQQVAWVTRA
                     SVAQPLADEALAITDGRTDIDRLLALISWRATHRAPRWLARRRATSAAAVDLAQRLLD
                     HERLVDACSLLAVDCLETGDRSGYGRALATLRWAAATEANPRLLWLAATLSAGEALLD
                     DDMEAAEQHRTDADRIGDAHDLPGWVAAELLFAAEMTLGSGDAGEMRRYLVPDNSPIL
                     DSPIARSSVAFMSALLGDDDRTLRYAETALRDVDEESSLLLCLVLLARAAATLGSDRL
                     ITELTTRLEPWRGHVAIDASGWWCAGPVDLVLAEMESAAGRTDRAAESLAQAVSIIET
                     VGDRRAARRADELKRGLGNGPLPRPPVTSAATHTGLAELTDRERDVLRLIATGMTNAA
                     IGSALAYSPSTIRADTVSIYRKLEVRGRAEAAAVAVAAGLTQPG"
BASE COUNT         1177 a         3309 c         3309 g         1293 t
ORIGIN      
        1 ggtggaccgt tggccgggag cctggccgag accgccgctc tcgccgccgc gacgggctcg
       61 acggcggtcg gcgtcgtggc cgacctgagc gacccgagcg accgggcccg agtggttccc
      121 gaggccgtcg acgcgctcgg cgggccgatc gacgtgctcg tcaacaacgc ggccgccgcc
      181 atctacggcc cgctcggcga gctgaccctg aagcgtcgcc gcctgatgtt cgaggtcaac
      241 gtccacgccc cggtcgacct ggcccagggt gtcgtcccgg ccatggccga ggccggtgag
      301 ggctggatcg tgaacgtgtc cagtcgcacg tcgcggccgt ggccgggacc gccgttcgag
      361 ctcggggtca ccgggaccac caccggcgcc tacggcgcgt ccaaggccgc cctcgaccgc
      421 atgaccaacg ccctggccgc cgagctcgcc ccgacgggga tccgggtcaa cgcggtggcg
      481 ccgcgtgccg cggtgatgac cgagggggcc gaggcgctgg tcggggaccg catcggcgag
      541 gaccggatcg agtcgctcga gcacatggtg gaggcggtgg tcgccctgtg ctgcggcggg
      601 ccggagctga ccggccggaa cgcgaccagc cacgagatca tcgacgagct cggactcgcc
      661 gtccgatccc tcgaagggac gtgaccgccg tgcgcatcat ctacttcgac atcgacacgc
      721 tgagggccga ccacctgggc tgctacggct accaccggga caccagcccc aacatcgacg
      781 cgctggccgc cgaggggatc cgcttcgagt cggtccacgc gtccgacgtg ccgtgcctgc
      841 cgagccgcac cgcgctcgcc accggcacgt tcggcacccg caacggagtg gtcaaccacg
      901 gcggggtggc cgccgacctg cgcgtcgagg gcgccgaccg ccagttcgtc agctccacca
      961 tgcgctcgtc gtgggcatgg cggctctaca ccgcggggtt ccacaccgcg tcgatgtcga
     1021 gcttcccgtt ccggcactcc gcgagctggt ggaaccacgg ctacatggag gccgccaacc
     1081 tgatgcgggg gatgggcgtc gagcgctccc cggcggtgac cgagcacgtc gtcgactggc
     1141 tgcggcgacg gggccgggcc gaccgctggt tcctgcacgt gcacctgtgg gacccccaca
     1201 cgccctacaa cacccccgac gactacggga acccgttcga gggcgacccg atcccggcct
     1261 ggcacaccga ggaggtccgc gccgccaact gggagcggcc cggcccgcac tcggcgcagg
     1321 agccgtgggg gttcaccccc gacgagtggg gaccgccccc gccccgccag ccgtgggacc
     1381 tgtcgagcat ggatgacgtc aaggccgcgt tcgacggata cgacgtcggc atccgctacg
     1441 cggacgacgc ggtgggcgcc gtcgtgtccg agctggccga cctgggagtg ctcgacgaga
     1501 ccgccgtgct ggtcagctct gaccacggcg agcagttcgg cgagctcggc gtctactgcg
     1561 accaccaggg cgcggatcag gccaccaccc acatccccgc cgtgctgcgc tggcccggcc
     1621 tcgaaccccg ggtgcagccc gggctgcact accacctcga cctggccgcg accgtcgtcg
     1681 agctggccgg tgcggtggtc ccgaccgacg tgtgggacgg cgagtcgctc gcccccgccc
     1741 tgcacgccgg caccaccgcc gggcgcgacc acctggtgct gacccagggg gcgtggaccg
     1801 cccagcgggg cgtgcgcacc ggcgaccacc tgtacctgcg caccttccac gacggctacc
     1861 acggctggga cgacgagatg ctcttcgacg tcgcggccga tccgcacgag atgaccgacc
     1921 tgatcgacga gccgtcggcc gccgcggcgg ccgaccgggc ccgctcggtg ctcgacgact
     1981 ggaccgcgac ccaactcgcc cggtcagggg ccgaggaccc gatcgacacc gtgctggccg
     2041 agggcgggcc gtaccacaca cggggtcatc tccccgccta cctgcagcgc ctgcgcgaca
     2101 ccggtcggtc cgggtgggcg gacctgttgg ccgagcgcca tgccgccgaa gccgacccgg
     2161 gccgttgggc cgacttcgag cgcacccgtc gacggctggc ctacgacctc atggggatcg
     2221 ccgagccgag ttgaaccgct cggcggcgag ctcgacgaag cgggccgcca acggcgacag
     2281 ggggcggtcg cggtgcacga tcccgatggt gcgctcgatc ggagggtcga ggggacggac
     2341 ccggaccgcg gcggtgtcgg agccggcggc catggatgcg ggcacgatgg tcgagccggc
     2401 cccggcggcg accagagcca ggatcgcctc acggtggtcg acctcgagcg cccgctgcgg
     2461 tgagctcgac gcggcggcga accaggcgtc gatcacctgg cgggtcgggc tcccatcggg
     2521 aggcaccacc agcacggcgc ctccgagccg atcggcccgg acgggtcgcg gtcggggtcg
     2581 gcggcgctga ccggaccccg cggtcgggcg gggcccatcg aggtcgggtt cgacccacac
     2641 gtagggctcg tcccacaggg cccgccagcg cagcgacggt tcggcgacgt ctccgatgcc
     2701 cagatccgcg acgccgctgg cgaccagggc gccgacggag ccgtcgccgg gggagcgcag
     2761 gtgcaccgag acgccggggt ggtcccggcg gaactcggcg acgagcccga cgacggtccc
     2821 gaccaccgtg ggttgcaccg ccaggtcgag gtgacccgac agtccgtcgg ccacgctcga
     2881 caccgcccgg cgggcgtccg cggcggcccg cagcaggtcc cgtgccgccg gcagcatcgc
     2941 gaggcacgcg tcggtgggga cgatgcgacg cccgatccgg tcgaacaggg cgatgcccag
     3001 gtcgcgctcc agccgcccga tcgacgccga gagcgtcggc tgggacaccg agcactcgat
     3061 cgcggcttcg gtgaaggagc cctcctcgac ggccgcgacg acgtagccga gctggcgctc
     3121 gctcagcgac gggagctcat ccatagtcgg acactatcgg tgtagaggag gacattgttg
     3181 gacgcgatgg atggtcgacg ggtggagtgg ggtcgtgctg tggctggaga tcgccctgat
     3241 cggactgggg atcggattcc tcggcggact gttcggcaag ggtggctccg cggtggccac
     3301 accgctgctg tacctggtcg gggtgccggc catcgccgcg gtggcctcgc cgctgcccgc
     3361 caccatcccc ggcaccctgg tcgcggccgg ggcctaccgg cgggcgggga tgatcgagct
     3421 gcgcacggtc ggctggagcc tggccaccgg tctgccggcc acggtcctcg gcgcgctcgc
     3481 caccaactgg atcgacggct cggcgctggt caccgccacc gagatcatcc tgatcgggat
     3541 cgggctccgt ctggtcatct cgcgccacgg gctcggcgcc gacccgaccg gcgacgaatc
     3601 cccctcgatc gagcggtggc ggttgctgct cgtcggcggc ctggtcgggt tgatcggtgg
     3661 tctgctggcc aacagcggcg gattcctgct cgcaccgctg tacctggcgg tcgcccacct
     3721 gcgcatcaag cccgctctgg ccgccagcct ggccgtctcg gcggtgctcg cggtgcccgg
     3781 caccgtcgtg cacggcttcc tcggccacat cgactgggcg gtggccgcca cgctggcggt
     3841 cacctcggtg ccggcgtcgc ggctcggtgc caccgtcgcc atccgcaccg cctcggaccg
     3901 gctcgagcgc ctctacggcg cggcgctggt cgtgctcggt gccggattgc tcctggtgag
     3961 ctgatcggcg ggtgcggcct cagtagggcg gtcgggtggg gtcgacgttg ccctcgccga
     4021 cgtccgcggt gaccttggtc aggccgtcgg cggggcagtg gaagccgtcg ccgtcgtcgc
     4081 cctcgccgcc gatctcgggg tagcgacgca cgaacttccc gtccttgacc atgaccaaca
     4141 tgctgcacgt gctggccttc atcgtcggtc cctcgtcggg gtcctgcggg gcgtgcagcc
     4201 cgccgccggt ccagtccgag gtcgccgccg cggtctccat gatgcagttg cggctgatct
     4261 caccgccgtt gtcggccgcg cacttgttgg cggcggtgac gaacagcagc cacgccgaca
     4321 tcgactgcat gccgagcacc gcgacctcgc cgccgtcgac gtactggtcg atcaggtcca
     4381 ggtacttgcg caccgccggc cacttgtcgg cctcctcgag catgtgcggc gccatgcgca
     4441 ccagggagcc gtcgagcgcc ttggggctgc cggactcgat gaagagcttg tcgtactggt
     4501 tggtctcgag cagcgcccgg cccttccagc cctggtcctt cagcttctcg agcagcttgg
     4561 cgatgtcggg cgactcgccg gtccagctga gcgaggtcgc cccggagtcg atgaccgaca
     4621 gggccagcgg cgtccagtcg gtggtgccga tcggcgggta gttgaagtcg ccgacctggc
     4681 tcatgccctt cacgtcgggg atgatcgcct cctgcttggt cttgacgatc ttgagcgacg
     4741 gcaggttgcc ccacaccggt gcccacttgg cggcctggtc cgggtagagg gcgaagaagt
     4801 cgcggaacca ggtgttggag atcgtgttcg tcgggttcgg gatcggctgg acctgcccgt
     4861 tgctcatcga cttggcgctc gacacagcga acgccgggat gtcgatgagc ttgcagcggt
     4921 ggaagtccga gccgtccttg cccgagaact gcaggtcgtc ctggacgttg cccccgccga
     4981 ccagggcgaa cgcgttgccg caggccttct gcatggcggc ttcgacctcg aacagcttgc
     5041 cgtccatgtc gatcagctcg atcttcaggc caccgatgcc gccctgggcg ttgcaccacg
     5101 ccgcgaacgc caccgacgcg tcccagatcg gcttctgcag ccccggtcgg atggtcgcac
     5161 cgcgatcgtt ggggaccccg atctggatcg tgtcggcccc gccctgctgc tcgccgtcgg
     5221 cgaccttggc gtcgcccttt ccgcacggcg ccttcaccgt cccgaagtcg ggcgagtcga
     5281 ccgccgcgcc gtcgtcggcg ggggcggtgg ccgccgcccc tccctgcggg gtgccgttca
     5341 tctccgcgcc gtcggtgatg tgctcgccgc cccgggcgcc gcacgcaccg gcgaggaccg
     5401 cgagggccga cacggctgcg accacggcga cgaagcggcc gcggcggagg ggtgacaagc
     5461 gagctgtgct ggacatcggc gactctcccg gagctgacca ctggcggggg aggggtcccg
     5521 gatcccgcgg ccgggggcca cggtcggggc cgtcgccgcc acgaacgtcg acagtctgct
     5581 ccgcgggatc cgcagcggtc gaacccggtc gtcgtcggtg cggggtcggc cgtcggcggg
     5641 tcgccgtcct aggttgagcc gccatgcagt ccgacgtcga cttcctcggc atggcgcccg
     5701 gtccgggtgc cgaccggttc cgcttcacca tctgcggcca cctggcccgt ctcgacggtc
     5761 tcctctacgg cgggaccgcg atcgccgcgt cgatggcggc ggcggaggcg gtgaccggcc
     5821 ggtcctcgct gtggaccacc acccagttcg tcgccaccgc cggcccggga accgaggtca
     5881 ccgtcaccgt cgaggagctg gccgccggtc ggcgcgccgc ccaagtgcgg gtgacgggga
     5941 ccgacccgac cggccgggtg ctgttcgcgt cgctcggcgc caccggcgag gccgacatgg
     6001 agcgaccccg cggcgtgttc ctggacccgc cccgggtcgc ggatcccgac gacagccctc
     6061 cgtggggcgg acccatcgcc acgatcgtcc gcctgatcgc ggacgccgag gagtcgtcgg
     6121 ggcgtccgcc gggggtgggg ttcatgtcgg tggtcgagct gctcgaaccc gaggtcgtct
     6181 cgcaccccga tccgggtccc ggacgactga cctactgggc ccgacgggtc gacggcggtc
     6241 cgatgacccc ggcgatcgtc gcctacatcg cggacctggt gccgctgtcg gtatcgaccg
     6301 cgatgggtgt ggtcgaggtc ggcaccagcc tggacaacac gatccgggtc gacccgtcgg
     6361 tccagaccga ctgggtgctg ctcgacctgc ggccccacgc ctcgatcggc gggtgctgcc
     6421 acggtgaggt gcacgcctgg gcacccgacg gtcgactgct ggcgaccgcg agccagaccg
     6481 cgctgctgcg gccgctcgat ccggcgctgg tcgcggcgtt ccgggccggc ccgtcggact
     6541 gaccggcggg tttctgaggt cgggattgcg acaaggtgtg tcggtgagga cccctatatc
     6601 ggccagactg gcggggtgga gcctgagctg agcgtccggc accgaccggt gcaggcccgg
     6661 ggccgcgagc gcttcgagct gatcctgcgc accggtcgcg accagctcgc ccgggtcggg
     6721 gtcgacggct tcaccttcga cggcctcgcc gatgcggccg gcatcccgat cgggtcgatc
     6781 taccagttct tccccaacaa gcacgcgctg atctgcgagc tgtccgccca ggacaccgac
     6841 gccatcgtcg ccgccctcgg ccgggccgcc gagcagttcc ccagcgacga ctggcagacc
     6901 gacgtcgacc ggctgatcga ccacctggcc gacctgtggc gctccgaacc cggccgtcgc
     6961 cacgtctggc tcgccatgca gtccaccgct ctgacccggt cgctggccgc cgagcagacc
     7021 acgcggatcg ccgagcagat cgtgcccctg gtcgcctggc tgatgccgga gggatcccgg
     7081 cgccgggccc gcaccgtcgc cgccgtcgtc gtgcagatgt gctactcgct gctgcacttc
     7141 tcggtgcacg acgaccggcc gcacccggcc acggtccggg agctcaagcg ggcactgagg
     7201 tcctacctcc gggcggtcgc cctggacgcc cagggctgag atgcgggccg ggcccggtca
     7261 gccgggctgg gtcaggccgg cggcgacggc caccgcagcg gcctccgccc ggccccggac
     7321 ctcgagcttg cggtagatcg agaccgtgtc ggcgcggatc gtcgacgggc tgtacgccag
     7381 cgccgacccg atggcggcgt tcgtcatgcc ggtggcgatg agccgcagca cgtcgcgctc
     7441 acgatcggtc agctccgcca gacccgtgtg cgtcgctgcg gaggtgacag gcggacgggg
     7501 aaggggtccg ttcccgaggc cccgcttcag ctcgtcggcg cgtcgagccg cccgacggtc
     7561 gccgaccgtc tcgatgatcg agaccgcctg tgccagcgac tcggccgcac ggtcggtccg
     7621 acctgcggcg gactccatct cggccagcac caggtcgacc ggccccgcgc accaccatcc
     7681 gctggcgtcg atcgcgacgt gtccccgcca cggttccagc cgggtcgtca actcggtgat
     7741 caggcggtcg ctgcccagcg tcgccgcggc ccgggccaac aggaccaggc acagcagcag
     7801 actggactcc tcgtccacgt cgcgcagagc ggtctcggcg tagcgaaggg ttcggtcgtc
     7861 gtcgccgagc agggccgaca tgaacgcgac gctggagcgg gcgatcgggc tgtcgaggat
     7921 cggtgagttg tccggcacca gatagcgacg catctcgccg gcgtcgcccg atcccagggt
     7981 catctcggcg gcgaacagga gctcggcggc cacccacccc ggcaggtcgt gggcgtcgcc
     8041 gatgcggtcc gcgtcggtgc ggtgctgctc ggctgcctcc atgtcgtcgt cgaggagggc
     8101 ctcgccggcc gagagggtgg ccgccaacca cagcagacgg gggttcgcct cggtcgccgc
     8161 cgcccaccgc agcgtggcca gcgcccggcc gtaaccgctg cggtcgccgg tctccaggca
     8221 gtcgacggcc agcagggagc aggcgtcgac caggcgttcg tggtcgagca gccgttgcgc
     8281 cagatcgacc gcggcggcgg acgtcgcccg ccgtcgggcg agccaacggg gggcacggtg
     8341 cgtcgccctc caggagatca gagcgagcag gcggtcgatg tcggtgcggc cgtcggtgat
     8401 cgccagcgcc tcgtcggcca ggggctgggc gacgctggcc cgggtcaccc aggcgacctg
     8461 ttggtcgggg tcgctcgacg cggggatccg catctcgacg agcgcccgac aggccaggat
     8521 cgctccgcgc cgggcgccgt cgtcgagtcg ttcggccagg tcgagcaacg ccgcgctgtg
     8581 acggtcaccg gcgcgccagt cggccgggaa cgtggagcgg atggcggcat cgaccaacag
     8641 gtcgacgtcg ccggcggtct ccgccagggt cgccacctcg tcgagcaggg tccgtgcgcc
     8701 gaccaggtcc ccggatccgg ccgtggcccg agccaggttc agcagcagcc gggcccgttc
     8761 gacgccgcgg tgctcggaga gctcgagtgc ggcctgcagg aggtggcgag cgacgtcgtg
     8821 gttcccggcg ttgaccgcga accgaccgga cgaggcggcc aggctcgcca gctcggcgcg
     8881 tcgctccggg gtggcgtgcc gtgccgcgtc gagcatggcc gccatgcgat cggcggtcgc
     8941 gtcgtcgagc agaactcgcc attccggttc gatcggacgc ccccccgttc ctgatcggcg
     9001 caccggcgcc gtcgtctgcg ttctggcgtg accctagggg acgccggtcg tatacgcccg
     9061 ctgtccgttc ggacatgacc ggtgcggg
//
LOCUS       DAOQOD010000127         9087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2812_length_9087_cov_11.918180, whole genome shotgun sequence.
ACCESSION   DAOQOD010000127 DAOQOD010000000
VERSION     DAOQOD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_9087_cov_11.918180"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PLZ72_11755"
     CDS             <1..1401
                     /locus_tag="PLZ72_11755"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNA37017.1"
                     /translation="VIGLLVVAILATLAVGVVVVATRGTGGDDDRAAPKLAADEDRTV
                     HPESLPAEAERLDVEGSSEGRYGRDMRAAIADVDGFWKREFSDTFGERYRTVQGGLYA
                     VTDRDRRVPCTESATDVEGNAFYCPSEDVVAWDADDLIPHMVETYGPLGLGLVIAHEW
                     GHAVQSRAGESEQAPVLVLEQQADCYAGAWVADVRRGGDRRYFVVNDRTLDLALAGFL
                     ELRDTPGTTRADAGAHGTAFDRIRAFQEGVDGGPSTCGYTADDLAPRLVDLPFTDQAD
                     FDSGGNLDLQDAFDLTVADLEDFWTEAWSSVGGDGRFDAPEVKVFSTKDRPRCDRPVG
                     LSKEVFYCADTNVLAVQEDGLVTELHARIGDYALGEVVGSGYSLAAMHQLGILDRDAA
                     SRTRTADCLAGVWSASVFSRNRESAQLQLSPGDLDEAVSALLSTAERREGSGSGAERV
                     AAYRDGFMDGADACLR"
     gene            1425..2180
                     /locus_tag="PLZ72_11760"
     CDS             1425..2180
                     /locus_tag="PLZ72_11760"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA37018.1"
                     /translation="MTDQHPILPGAEPLSVAGGPVGVLVLHGFTGNPTSMRGLAEAMV
                     AAGHTVEMPRLPGHGTHVDDMLRTSWADWSAAAEAALVDLEARTDEVVVAGLSMGGSL
                     TAWLATRHPELSGIICINPAVAAQPDMLAAVAAMVEAGETVMDGIGSDVAAPDVVESA
                     YDKVPLAPLLSMFEVAEEIVGDLGRIECPLLLFTSPQDHVVPPTDSDLLAASVSGPVE
                     RVSCDRSYHVATLDLDKELIFERSVEFVGRVTA"
     gene            complement(2273..2578)
                     /locus_tag="PLZ72_11765"
     CDS             complement(2273..2578)
                     /locus_tag="PLZ72_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HNA37019.1"
                     /translation="MRNSLRYLLVGFAFVAGGILALSGVAGAQDDSSDAYVLSNTASR
                     TAELPATASAASAESASASANALAFTGGDTMVLAAVGGVAVLAGGAILVARRRVAQA"
     gene            complement(2651..4438)
                     /locus_tag="PLZ72_11770"
     CDS             complement(2651..4438)
                     /locus_tag="PLZ72_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNA37020.1"
                     /translation="MPGRPRPPRRRLRVLAVIGSLVLVWLGVVAFRLGTAAVDARRGV
                     LALERVSERSTGDLGRFVGSVGQPDDPDTEAVMDDLATASDAFRSAHGAASSALVAPL
                     RLVPILGRQVRSVASMSGAAATAASASRTAFSQLAGIAAEDSATPQERLDQVTRTREV
                     LAELDSTTSSLDLGPKDGLIGPLADAYDRFDAELTETRGTVTRALAGATGVDRFLTGP
                     SRYLVLAANNAEMRAGSGMFLQAGELTASGGQLELSDMQPTARMVLDTPGTTLDPDVA
                     ALWDWMDPAREWRSLNATPRFDQSARMAADMWAASGNPPVDGVVALDLVGLQRLLELV
                     GSVDVVDTDGRPMTVTADNVLSEMLLKQYLGSDTVDERRDQLSVVAQAVFRALNERGY
                     EPGQLLAALQSAGRGRHLMMWSNDPVEQAGWEALDASGALPRDALLLSVLNRGGNKLD
                     QFLRVTADMTWTEGTGSRRVSVAITATNTTPAGLPRFVAGPFPGLDTEAGEYRGVVAL
                     TIPKTATAPAAEGAELFLSGEDGPTRLIAVRIALKRGESSSVTLSFDIPTRTDPVVVL
                     PSARVPPVVWTAGGDTWKDRLPHPVQLGG"
     gene            4517..4819
                     /gene="gatC"
                     /locus_tag="PLZ72_11775"
     CDS             4517..4819
                     /gene="gatC"
                     /locus_tag="PLZ72_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNA37021.1"
                     /translation="MESRITTDDVAKVARLARLRLTDDELDRFTHQLSDILDHAADIE
                     ALELDDVEPMARPVPLSNVLRSDEPTDPLDRDAVLAAAPSTEDGQFRVSPVLGEAP"
     gene            4816..6237
                     /gene="gatA"
                     /locus_tag="PLZ72_11780"
     CDS             4816..6237
                     /gene="gatA"
                     /locus_tag="PLZ72_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNA37022.1"
                     /translation="MRTAVDIAAGVRSGELSALEVLDEHLERVALRESEIHAFNHLTE
                     EAARAAATELDRRVAAGEDPGPLAGVPVALKDNLCTEGVPTTCSSRILEGWIPPYTAT
                     VVERLVAAGAVPVGKTNLDEFAMGSSTENSAFGPTRNPHDVTRVPGGSSGGSAAAVAA
                     GFVPVSLGSDTGGSIRQPAALCGVVGVKPTYGTVSRLGLVAFGSSLDQIGPFSATVAD
                     AARVLEVIGGHDPGDSTSLPGSFPSVSEHLDDGVSGLRVGIVTEMMGDGIAADVTARV
                     RAAADALAAAGATVEEVSVPAVTYGLSAYYLVAPAEASSNLARYDGVRYGVRVEAATA
                     GEMMEATRTAGFGDEVKRRIMLGTYALSAGYYDAFYGKALKVRTLIRRDFDAAYERFD
                     VLLSPTSPTTAFPFGDKTADPMAMYLNDVCTIPTNLAGHPAMSVPFGVGDDGLPVGVQ
                     VLAPALGEVVMFRTAAVLEGSVR"
     gene            6234..7652
                     /gene="gatB"
                     /locus_tag="PLZ72_11785"
     CDS             6234..7652
                     /gene="gatB"
                     /locus_tag="PLZ72_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNA37023.1"
                     /translation="MSLDLDVWEIVIGLEVHTELLTRTKLFSGAVNRFGGEPNTHIDP
                     MTLGLPGALPVLNEQAVELAIRVGLALNCTVQRSVFARKNYFYPDQPKNYQISQYELP
                     INADGWLELPSGKRVGIERAHLEEDTGKSTHVGGGGRIHDARYSLVDLNRAGVPLLEI
                     VGRPDLRTADEAKAYVSELRSILVAVGASDGKMEEGSLRVDCNVSVRHRGDSDFGTRC
                     EIKNINSLRAMGRAIDYEAARQADLISSGEAVVQQTRHWNDTEGRTTLLRSKEDADDY
                     RYFPDPDLVEIDPGPEWIERVRAALPMLPSARRHRLAERTGADLERTALVVERGLDEL
                     CLTAVDAGADPERVLTHAEHNLADERAAGLDPEAFAQLVAMETDGRLTATQTKAVLAE
                     MVETGDGPDAIAARHGYEAMDSGSLEPIVDGLIEANPAEWERFCDGDAKVAGFFVGQV
                     MKATQGKADGKAVTALLNARRG"
     gene            7663..8397
                     /locus_tag="PLZ72_11790"
     CDS             7663..8397
                     /locus_tag="PLZ72_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA37024.1"
                     /translation="MPSPAEFALLAGALFVASAVNALVGFGFALTSVPLMALAVGPRD
                     AVVLSALMGMLSSGGVALRHREEVARPIAFRLVAGSLVGMPIGLFVLVAVPEQWIGIG
                     IGVVVLAAVVLLAGGWQVERIPAWADLVAGLVSGVLNTSVGVGGPPVVTALQGHGLDK
                     ARFRATTTAVFAAGGLVSLVLFALSGQIERSVAAVAVLALPAWPAGWWVGDRLHHRFP
                     EERFRTLVLVMLAATAVLSLLSAVRG"
     gene            8481..9026
                     /locus_tag="PLZ72_11795"
     CDS             8481..9026
                     /locus_tag="PLZ72_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37025.1"
                     /translation="MGAFAVLVVLSPLGACSRQSGTEELRSDPADETVPGPAPTAPPV
                     AAGAEGSSGADVDVNELIRRIDALNSETDVCTLLTGQAIRDVTRSNVNLTSLLTNPSG
                     FTQLFAALDRLFAHLVQIGPPDLAPSLQTMQGVWKSMSSIDPRSPDAESRAGKLIGDP
                     KVQAAQDSIGSYVKATCAPTG"
BASE COUNT         1262 a         3247 c         3256 g         1322 t
ORIGIN      
        1 gtgatcggcc tgttggtcgt ggccatcctg gccacgctcg ccgtcggcgt cgtcgtcgtg
       61 gcgacccgcg gcaccggagg cgacgacgac cgcgccgcgc ccaagctggc ggccgacgag
      121 gaccggaccg tccaccccga gtcgctgccc gccgaggcgg aacgcctcga tgtcgagggc
      181 agctccgaag gtcgctacgg ccgcgacatg cgggcggcga tcgcggacgt cgacggcttc
      241 tggaagcggg agttctccga caccttcggg gagcggtacc gcaccgtgca gggcggcctg
      301 tacgccgtga ccgaccggga ccgccgtgtt ccctgcaccg agtcggcgac cgacgtcgag
      361 ggcaacgcgt tctactgccc gtccgaggac gtggtcgcct gggacgcgga cgacctcatc
      421 ccccacatgg tcgagacata cggtccgctc ggcctcggtc tggtcatcgc ccacgagtgg
      481 ggtcacgccg tgcagtcccg ggccggggag agcgagcagg ccccggtgct ggtgctcgag
      541 cagcaggccg actgctacgc cggggcgtgg gtggccgatg tgcgccgagg cggggaccgg
      601 cgctacttcg tcgtcaacga ccggaccctc gacctggcgc tcgccggctt cctggagctg
      661 cgcgacaccc ccgggacgac ccgggccgac gccggtgccc acggcaccgc cttcgaccgc
      721 atccgggcgt tccaggaagg cgtcgacggt gggccgtcaa catgcggcta caccgcggac
      781 gacctggccc cccgcctggt cgacctcccg ttcaccgacc aggccgattt cgactcgggc
      841 ggcaacctgg acctgcagga cgcgttcgac ctgaccgtgg ccgacctcga ggacttctgg
      901 accgaggcct ggtcgtcggt cggcggcgac ggccgattcg atgctcccga ggtgaaggtg
      961 ttctcgacga aggaccgtcc gcgctgcgat cgcccggtcg gtctgtccaa ggaggtcttc
     1021 tactgcgccg acaccaacgt cctggcggtc caggaggacg gactggtgac cgagctccac
     1081 gcccgcatcg gcgactacgc gctgggtgag gtcgtcggct ccggctactc cctggcggcc
     1141 atgcaccagc tcggcatcct cgaccgcgac gccgcgtcga ggacccgcac cgccgactgc
     1201 ctggccgggg tgtggtcggc gtcggtgttc tcccgcaacc gcgagtcggc gcagctccag
     1261 ctgtcgccgg gagacctcga cgaggcggtc tcggcgctgt tgagcaccgc cgagcgccgt
     1321 gaggggtccg gcagcggcgc tgagcgggtc gcggcctatc gtgacgggtt catggacggc
     1381 gccgacgcct gcctgcggtg accgccgaga gcacaggagc accgatgacc gatcagcacc
     1441 ccatcctgcc gggggcggag cccctcagcg tcgccggcgg acccgtcggc gtgctcgtcc
     1501 tgcacggctt caccggcaac ccgacgtcca tgcggggcct ggccgaggcg atggtcgccg
     1561 ccggccacac cgtcgagatg ccccgtctgc cgggtcacgg cacccacgtc gatgacatgc
     1621 tgcgcacctc ctgggccgac tggtccgccg cggccgaagc cgctctggtc gacctcgagg
     1681 cgcggaccga cgaggtcgtc gtcgccggcc tgtcgatggg aggctcgctc accgcctggc
     1741 tcgcgacccg tcaccccgag ctgtcgggga tcatctgcat caacccggcc gtcgccgccc
     1801 agcccgacat gctcgccgcg gtggcagcca tggtcgaggc gggggagacc gtcatggacg
     1861 gcatcggctc cgacgtggcc gcacccgacg tcgtcgagtc ggcctacgac aaggtccccc
     1921 tcgccccgct gctgtcgatg ttcgaggtgg ccgaggagat cgtcggcgat ctgggtcgca
     1981 tcgagtgccc gctgctgctg ttcacgagcc cgcaggacca cgtcgtgccg cccaccgaca
     2041 gcgaccttct ggccgcgtcg gtgtcgggac cggtggaacg ggtcagctgc gaccgcagct
     2101 accacgtcgc caccctcgac ctggacaagg agctgatctt cgagcgctcg gtcgagttcg
     2161 tcggccgcgt caccgcctga gcggcgaggg tccggacgcg gaactgcccg ccggctgggc
     2221 cggcgggcag ttcggacggt caggttgtca tgcgcccaaa ggccacgggg tctcaggcct
     2281 gggcgacccg acgacgggcg acgaggatcg cgccaccggc gaggacagcc acgccaccga
     2341 cggccgcgag gaccatggtg tcgccgcctg tgaaggcgag ggcgttggca gaagcagacg
     2401 ccgactcagc ggaggccgcc gaagcggtcg ccgggagctc ggcggttcga gaagcggtgt
     2461 tggagagcac gtaggcgtca gaggagtcgt cctgcgcacc ggctacgccg gacagagcga
     2521 ggatccctcc tgcaacgaat gcgaaaccca ccagcaagta acgaagactg ttgcgcatgt
     2581 cagcaaggta gcaccggttc gccgccgagt ggagcatgca gatgtaccca tctgttgggc
     2641 ccgaaggccc tcacccgccg agctggaccg ggtgcggcag acggtccttc caggtgtcgc
     2701 ctccggcggt ccacaccacc ggcgggaccc gggccgaggg cagcacgacc accgggtcgg
     2761 tgcgggtcgg gatgtcgaac gagagcgtga cgctgctcga ctcgcccctc ttcagcgcga
     2821 tgcggaccgc gatcagccgc gtcggcccgt cctcgccgct caggaacagc tcggcgcctt
     2881 cggcggccgg agcggtggcg gtcttcggga tcgtcagggc gaccacaccg cggtactcgc
     2941 ccgcctccgt gtcgagcccg gggaacgggc cggccacgaa gcgtgggagt cctgcggggg
     3001 tcgtgttggt ggctgtgatc gccaccgaca cccgccggga acccgtccct tcggtccatg
     3061 tcatatcggc ggtgacgcgc aggaactggt ccagcttgtt gccaccccgg ttcaggaccg
     3121 acagcagcag cgcgtcgcgg ggcagggcac cgctggcgtc gagcgcctcc cacccggcct
     3181 gctcgacggg atcgttggac cacatcatca ggtggcgtcc ccggccggcg gattgcagcg
     3241 cggccaggag ttgacccggt tcgtatcccc gttcgttcag cgcacggaag accgcctggg
     3301 cgacgaccga cagctggtcg cgccgttcgt cgacggtgtc ggagcccagg tactgcttca
     3361 gcagcatctc gctcagcacg ttgtcggcgg tcacggtcat cggcctgccg tcggtgtcga
     3421 cgacgtcgac ggagccgacc aactcgagca gacgttggag accgaccaga tcgagggcga
     3481 ccaccccgtc gacgggtggg ttgcccgacg cggcccacat gtcggcggcc atgcgggcgc
     3541 tctggtcgaa gcggggcgtg gcgttcaggc tgcgccactc gcgggcgggg tccatccagt
     3601 cccagagcgc cgcgacgtcg gggtcgagcg tcgttcccgg cgtgtcgagc accatgcgcg
     3661 ccgtcggttg catgtcgctc agctccagct ggccgccgct cgctgtcagc tcaccggcct
     3721 gcaggaacat ccccgacccc gctcgcatct cggcgttgtt cgccgcgagg acgaggtagc
     3781 ggctgggtcc ggtcaggaac cgatccaccc cggtggctcc cgccagggcc cgggtcacgg
     3841 ttccccgtgt ctcggtcagc tcggcgtcga agcggtcata tgcgtcagcc aacggtccga
     3901 tcaggccgtc cttggggccg aggtcgagcg aggaagtggt cgagtcgagc tcggcgagga
     3961 cctcacgggt ccgggtcacc tggtcgagcc gctcctgcgg cgtggccgag tcctccgctg
     4021 cgatcccagc gagctgggag aacgctgtcc gcgatgcgct ggcggcagtc gcggccgccc
     4081 cgctcatgga cgcgaccgac cggacctggc gaccgaggat cggcaccagt cgcagcggtg
     4141 cgaccagagc cgaactcgcg gcgccgtggg cggacctgaa cgcgtcggat gccgtcgcga
     4201 gatcatccat gaccgcctcg gtgtcgggat cgtcgggctg tcccaccgag ccgacgaagc
     4261 ggccgaggtc gccggtcgat cgttcgctga cccgctccag ggccaggacc ccccgccggg
     4321 cgtcgaccgc ggcggtcccc aggcggaacg cgacgacgcc cagccacacg aggaccagcg
     4381 aaccgatgac cgccagcacc cggagtcggc gccgtggtgg tcgtggtcgg cccggcacgg
     4441 cttcccacga tatcgacgcg cccgggctgt gcgtcggtcg gggatgtcga tcgcagctcg
     4501 gtagtgtcgg accccgatgg agtcccgcat caccaccgac gacgtcgcca aggtggcccg
     4561 actggcccgc ctgcgactga ccgacgacga gctcgaccgg ttcacccacc agctgtcgga
     4621 catcctcgac cacgcggccg acatcgaggc gctggagctc gacgacgtcg agccgatggc
     4681 ccggccggtg ccgctgagca acgtgctccg ctccgacgag ccgaccgacc ccctcgaccg
     4741 tgacgccgtc ctggcggccg cgccctcgac cgaggacggc cagttccgcg tgtcacccgt
     4801 cctgggggag gcgccgtgag gaccgcggtc gacatcgccg ccggcgtccg gtccggtgag
     4861 ctgagcgcac tcgaggtgct cgacgagcac ctcgagaggg tggcgctgcg tgagtcggag
     4921 atccacgcgt tcaaccacct gaccgaggag gcagcgcggg ccgccgcgac cgagctcgac
     4981 cgtcgtgtcg ccgccggcga ggacccgggg cccctggccg gggtgcccgt cgcgctcaag
     5041 gacaacctct gcaccgaggg cgtgcccacc acgtgctcgt cgcggatcct cgaggggtgg
     5101 atcccgccgt acaccgcgac ggtcgtcgaa cgcctggtcg cggcgggggc ggtgccggtc
     5161 ggcaagacca acctggacga gttcgcgatg ggctcgtcca ccgagaactc ggcgttcggc
     5221 ccgacccgca acccccacga cgtcacccgg gtgcccggcg gatcctcggg cggctccgcg
     5281 gctgcggtcg cggcagggtt cgtcccggtg tcgctcggct ccgacaccgg cggctcgatc
     5341 cgccagcccg ccgcgctgtg cggcgtggtc ggcgtcaagc ccacctacgg cacggtgtcc
     5401 cggctgggac tggtggcgtt cgggtcctcg ctcgaccaga tcggcccgtt ctcggcgacc
     5461 gtcgccgatg cggcgcgggt gctggaggtc atcggtggac acgaccccgg ggactcgacg
     5521 tcactgcccg gctccttccc gtcggtgtcg gagcacctcg acgacggcgt ctccgggctg
     5581 cgggtcggca tcgtcaccga gatgatgggc gacggcatcg ccgccgacgt caccgcccgg
     5641 gtccgtgccg cggccgacgc cctggccgcc gccggcgcca ccgtcgagga ggtctcggtc
     5701 ccggcggtca cctacgggct gtcggcctac tacctggtcg ccccggcaga ggcgagctcg
     5761 aacctggccc gctacgacgg cgtccgctac ggcgtccggg tcgaggcggc caccgcgggc
     5821 gagatgatgg aggccacccg caccgcgggc ttcggtgacg aggtcaagcg ccgcatcatg
     5881 ctgggcacct acgccctctc cgccggctac tacgacgcct tctacggcaa ggcgctcaag
     5941 gtgcgcaccc tgatccgacg cgacttcgac gccgcctacg agcgcttcga cgtcctgctg
     6001 tcgcccacgt cgccgaccac ggccttcccg ttcggcgaca agaccgcgga cccgatggcc
     6061 atgtacctca acgacgtctg caccatcccg accaacctgg ccggccatcc cgccatgtcg
     6121 gtcccgttcg gggtgggcga cgacgggctc ccggtcggcg tgcaggtcct ggcccccgcg
     6181 ctgggggagg tcgtcatgtt ccgcaccgcc gcggtgctcg aggggagcgt ccgatgagcc
     6241 tcgacctcga cgtgtgggag atcgtgatcg gactcgaggt ccacaccgag ctcctgaccc
     6301 ggaccaagct gttctcgggg gcggtcaacc gcttcggcgg cgagcccaac acccacatcg
     6361 acccgatgac cctcgggctg cccggtgcgc tgccggtgct caacgagcag gccgtggagc
     6421 tcgcgatccg ggtcggactg gccctcaact gcacggtcca gcgttcggtg ttcgcccgca
     6481 agaactactt ctacccggac cagccgaaga actaccagat ctcccagtac gagctgccga
     6541 tcaacgccga cggctggctg gagctgccgt cgggcaagcg ggtcggcatc gagcgggcgc
     6601 acctcgagga ggacaccggc aagtcgaccc acgtcggcgg cggcgggcgc atccacgacg
     6661 cccgctactc gctggtcgac ctcaaccgcg ccggcgtccc gctgctcgag atcgtggggc
     6721 ggcccgacct gcgcaccgcg gacgaggcca aggcgtacgt gtcggagctg cgttcgatcc
     6781 tggtcgccgt cggcgcgtcg gacggcaaga tggaggaggg ctcgctgcgg gtcgactgca
     6841 acgtgtcggt ccgacaccgc ggcgacagcg acttcggaac ccgttgcgag atcaagaaca
     6901 tcaactcgct gcgggccatg ggccgcgcca tcgactacga ggccgcccgg caggccgacc
     6961 tgatctcctc gggcgaggcg gtggtccagc agacccgcca ctggaacgac accgagggtc
     7021 gcaccacgct gctgcgctcc aaggaggacg cggacgacta ccgctacttc cccgaccccg
     7081 acctggtcga gatcgacccg ggtccggagt ggatcgagcg ggtgcgcgcc gccctgccga
     7141 tgttgccgtc ggcccgacgc caccgcctgg ccgaacgcac cggggccgac ctcgagcgga
     7201 ccgcgctggt ggtcgagcgt gggctcgacg agctgtgcct gaccgccgtg gatgccggcg
     7261 cggatccgga gcgggtgttg acacacgccg agcacaacct ggcagacgag cgcgccgcgg
     7321 gcctggatcc cgaggcgttc gctcagctgg tcgccatgga gaccgacggt cggctgaccg
     7381 cgacgcagac caaggcggtg ctcgccgaga tggtcgagac cggcgacggt cccgacgcga
     7441 tcgcggcgcg ccacggctac gaagccatgg actcgggctc gctcgagccg atcgtcgacg
     7501 ggctgatcga ggcgaacccg gccgagtggg agcggttctg cgacggcgac gccaaggtcg
     7561 ccgggttctt cgtcggtcag gtgatgaagg ccacccaggg caaggccgac ggcaaggcgg
     7621 tcacggccct gttgaacgcc cgtcggggct gatcgcccga ccgtgccctc accggccgag
     7681 ttcgccctcc tcgccggcgc cctgttcgtg gcctcggcgg tcaacgccct cgtcggattc
     7741 ggcttcgctc tcacctcggt gccgttgatg gcgctcgccg tcggcccccg cgatgccgtg
     7801 gtgctctcgg cgctgatggg gatgctgtcg agcgggggcg tcgcgctgcg ccaccgcgag
     7861 gaggtggccc gaccgatcgc cttccggctg gtcgccggct ccctggtcgg catgccgatc
     7921 gggctgttcg tcctggtcgc cgtcccggag cagtggatcg gcatcggcat cggtgtcgtg
     7981 gttctggccg cggtcgtgct gctggccggc gggtggcagg tcgagcggat cccggcgtgg
     8041 gcggatctgg tcgccgggct cgtgtcgggg gtcctgaaca cctcggtcgg agtcggcggt
     8101 ccgccggtgg tgacggcgct gcagggccac ggcctggaca aggcgcggtt ccgggcgacg
     8161 accaccgcgg tgttcgcagc gggcggactg gtctcgctgg tgctgttcgc tctcagcggt
     8221 cagatcgagc ggtcggtggc ggcggtggcc gtcctggccc tgccggcgtg gccggccgga
     8281 tggtgggtgg gggaccggct tcaccaccgg ttccccgagg agcggttccg cacgctcgtc
     8341 ctggtgatgc tcgccgccac ggcggtgctg tcgcttctca gcgcggtccg gggctgatgg
     8401 gccgctcgac gtcggctccc gtaccatcga cgcccgtggg gtccgctgca tcgcatcgtc
     8461 gtggtcggct cgcgaccgcc gtgggtgcgt tcgcggtgct ggtcgtgctg tcaccgctgg
     8521 gcgcgtgcag tcgccagtcc ggcaccgagg agctccgctc agatccggcc gacgagacgg
     8581 tgccgggccc ggcaccgacg gccccgcccg tggcggccgg cgccgagggt tcgagcggtg
     8641 ccgatgtcga cgtcaacgag ctgatccggc ggatcgacgc gctcaactcc gagaccgacg
     8701 tgtgcacgct gctgaccggt caggccatcc gcgacgtgac ccgcagcaac gtgaacctga
     8761 ccagcctgtt gaccaacccg tcggggttca cccagctgtt cgccgcgctc gaccggctgt
     8821 tcgcgcacct ggtgcagatc ggtccgccgg atctggcgcc gtcgttgcag accatgcagg
     8881 gggtgtggaa gtcgatgtcg tcgatcgatc cgcgctcgcc ggacgcggag tcgcgggccg
     8941 gcaagctgat cggtgatccg aaggtgcagg ccgcgcagga ctcgatcggc agctacgtca
     9001 aggccacctg cgcccccacc ggctgaccag cccgttctgt tcgttggatc acaacgcaga
     9061 cgttgtgatc caacgaacag aacgggg
//
LOCUS       DAOQOD010000128         9016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2847_length_9016_cov_10.935387, whole genome shotgun sequence.
ACCESSION   DAOQOD010000128 DAOQOD010000000
VERSION     DAOQOD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_9016_cov_10.935387"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..999
                     /locus_tag="PLZ72_11800"
     CDS             181..999
                     /locus_tag="PLZ72_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37026.1"
                     /translation="MQDHDPTTPDPHAGDTSDDALDHTGRVVVVTGGTKGIGRVLVER
                     FLGWGAEVVTCGRTEPDRLPSAGGRTASFVAADVRDPEQAAAVVDAAVERHGTVDVVI
                     NNAGGAPEADAATASPRFSESIVRLNLLAPLHVAQRANHHMQAGAGGVIVNIASVSGT
                     RPSPGTAAYGAAKAGLINLTESLAVEWAPKVRVVGIVAGLIATEQAELHYGDADAQAR
                     VASTVPAGRLGTPEDLAQACRWLTSPLASYVSGSSVWVHGAGERPAFLAAARGE"
     gene            1134..1730
                     /locus_tag="PLZ72_11805"
     CDS             1134..1730
                     /locus_tag="PLZ72_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37027.1"
                     /translation="MLVAFVLGGCSDLVQQADATVDIAAEDTNQATQLRQDLLQRAPS
                     WGGVRVAEDTDEQRGDISLTFSLPGRNLDAALGTIHSLDADVESQSIDVERGDVERTT
                     TTAKEGTPSAGDDGQVTLRVNISSQPAAAGAGAVLRLVMAVFSIIGMVATALWISRAW
                     KKRFGAEESPRPRRRNIDVDLSDPPTQETPQVPRGPWN"
     gene            complement(1822..>2736)
                     /locus_tag="PLZ72_11810"
     CDS             complement(1822..>2736)
                     /locus_tag="PLZ72_11810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA37028.1"
                     /translation="NGDDVQNPHIDIGTYGIWTGALDMVPSAVAGEATAELDELGYGA
                     VWIPETVFREPFVHAALLMAASPRIKVATGIVNRYGRDPLAANAAMQTLNEAFPGRFL
                     LGLGVSHEHLVAWVRKHDYSRPYSGMVEYLDAMAKARFFAPRGDPPAQMVLAALGPRM
                     LSLAAERTAGAHTYFVPPEHTAIARETMGPEPLLAVEQMVVLDTDPATARETARQHMA
                     VYLSAPNYTNNLARLGFGPEDVADGGSDRLVDAIVVHGDLDDVLGRIRSHLDAGADHV
                     CVQVLHPDMAHLPRAEWRELSAALGLTG"
     assembly_gap    2737..2836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2848..4374)
                     /locus_tag="PLZ72_11815"
     CDS             complement(2848..4374)
                     /locus_tag="PLZ72_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNA37029.1"
                     /translation="MSPSAAELDLAVATLVDPSLASIVEMVLTRRDGAYEAHAVDGSV
                     RFRRHDPAAAPDGSAGPARDWSFTVDAVTGRDPLGDRATDRFAGLDAELAARFPERAA
                     NAYPFAFEQVAQLFDHPHAPDLCVVHTASHHWAEQGGHIGEHGSLGAVQSRAPFLISG
                     PGARSDGLVAGPVAQMVDIAPTVLALLGVDPSTLAGADGRPIDAVLDRAALDRGDGPR
                     HVVGFLLDGCNPNVLYDSVARGEAPHIAGLLADGTALRDGIVSSLPTVTLPNHTTVIT
                     GRHPGHHGILHNAWWDRDRREQVITNSPETWATAMDSLSPGTDSIHHALRRARPGCFT
                     ASVNEPADHGAVYSTFDLMRRGVRMGDFPRSAEGLPHSSEMFVRPVKDYALFTQVDHH
                     GTEQAVGIVDGRYLDTDHPLPTFLWVNYALTDSAFHEGGPHSEIARASIRDTDGRIGE
                     VLAALERRGIREQTTVFLCADHGMEESDPAVGGDWSPALAEAGLDVRDEAFGFLYINP
                     "
     gene            4830..6242
                     /locus_tag="PLZ72_11820"
     CDS             4830..6242
                     /locus_tag="PLZ72_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA37030.1"
                     /translation="MSGSSSTRDDLVRAGVELLDQLPLDRILAGVTTAAVAEHAGVTT
                     GSFFHHFRNSSEYTAAVVRSFDRERRFERDAVSDLTDAIQHRRLSEAIAAVLVTTWRI
                     LADDPQRQADRRGQMHLFAHHRVELPPNGTDPTPSGPTETVGDVLRDIYRSQVDQITA
                     TWQRLLSLGEMRLAEPFDTRRLAVAVHALLLGLEIVHAVDPDAVDDNLFAEATATLAA
                     ALSRLDLDPARVMTAVELGQTEATSPQARSGARRRLDTRRRIVAAATGMFDSGWDHVS
                     AKEIGATAGVSTQTVINLFGNVRRVCAATFVRHLPAVEAALGSTGQDRPRAGVAAALG
                     VIADAAAGDPHPARALLAERVGVRVERGFELDDDDIRVIVPIGIRIAQQITRVTGLDI
                     ASPHVPDLSSLVVDFTLGHAVPRPDSRDDTVRRAMALLDDALGELPIDSEPPGVPHYS
                     DDPSDLRHDTPDARGNGRLQ"
     gene            6239..6745
                     /locus_tag="PLZ72_11825"
     CDS             6239..6745
                     /locus_tag="PLZ72_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNA37031.1"
                     /translation="MSTRRVPAIDGWFTMPTDGSAPALIGTRDTTTGTYFFPPERASS
                     RAPGGADAELVDVELSRTGRLWSYTDAGYRPPEPYVPVTDPHVPFAIAAVELAEEKMV
                     VMGQVVAGVAVDDLSVGMEMELVLDVLYSEPDPDGADGDVVEHVVWKWRPVGGTNAAG
                     AVSAGEPS"
     gene            6742..7941
                     /locus_tag="PLZ72_11830"
     CDS             6742..7941
                     /locus_tag="PLZ72_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNA37032.1"
                     /translation="MTVDQVAVLGVGMHPWGKWGRNFVEYGVAAARAALADAGVDAAD
                     IQFIAGADTMRNGYPGYVAGATFAKALGWSGIPVTSAYGACASGTMAIEAARQRILAG
                     LCDVALVVGADTTPKGFLKPNKGERGDDPDWLRFHLMGATNPTYFALYARRRMDLYGA
                     TLDDFAQVKIKNARNGLGNPYARYRKEVTVADVEASPTVASPLRLLDICATSDGGAAI
                     VLSSMDYARRLRDGSDPTVRIAAVSTVTPTFPNTIIEMPDFATDSAAGIAAPELTFKQ
                     AIGAAAYEQAGLGPDDVDVAEVYDLSTALELDWYEDLGFCGPGEAEKLLRDGVTQLDG
                     RLPVNPSGGLACFGEAVPAQAIAQVCEITWQLRGQAERRQVADPTVGITANQGLFGHG
                     SSVILTR"
     gene            complement(8145..>9016)
                     /locus_tag="PLZ72_11835"
     CDS             complement(8145..>9016)
                     /locus_tag="PLZ72_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37033.1"
                     /translation="APWGVATGIVVVLSLGGAVDVIANVAATAGLADRPGHLVRLHAV
                     FNLGGAAGTVVVGVLLAVAGAAGWRWAWVVVAVLVVGVVVVTAGVHLPAGGAGSAVRF
                     SDGMRELRSQRLVPVAVALALAAVVEGGVNTWGVLQLRGQLDAGLLVGAGGAFVGYLV
                     AVTARLSVSRVETAAAARRAVIGGTVLAAVGLVVLATVSQPVLAAAGLVLAAGGVSVC
                     WPLLMSEVGRGRERPGVLVGAVTTFGYLGSVIGPGLVGAVSGAIGVPAGLLVLAGAAI
                     AVAALVGARRGMR"
BASE COUNT         1322 a         3392 c         3090 g         1112 t
ORIGIN      
        1 cgcaggaagc gggcggcgga ctcgaactgg accgcgaccg cctcgagcgc gcccgggtcc
       61 gaacccagcc gagcgcccgc cggtcccgtc gcgtcacctg ccgccatcgg gcgcgacgct
      121 acggagcccg cggtcggctg tcaccgggga ccgcgacccg gtcggggtac cgtctcggcc
      181 gtgcaggacc acgacccgac gacacctgac ccccacgccg gcgacacgtc cgacgacgca
      241 ctcgaccaca ccggccgcgt cgtcgtcgtc accggcggca ccaagggcat cgggagggtc
      301 ctggtcgagc gcttcctcgg ttggggtgcc gaggtggtca cctgcggacg caccgagccg
      361 gaccggctcc cgagcgcggg cggccgcacc gcgtcgttcg tcgccgcgga cgtccgagat
      421 cccgagcagg ccgcggcggt cgtcgacgcc gccgtcgaac gccacggcac cgtcgacgtc
      481 gtcatcaaca acgccggcgg agcacccgag gccgacgccg ccaccgcgtc gccccgcttc
      541 agcgagtcga tcgtccggct gaacctgctg gcaccgctgc acgtcgcgca gcgcgccaac
      601 caccacatgc aggccggcgc cggcggcgtg atcgtgaaca tcgcctcggt gtcgggcacc
      661 cgtccttcgc cggggaccgc ggcctacgga gcggccaagg ccggcctgat caacctgacc
      721 gagtcgctgg cggtggagtg ggcgcccaag gtgcgggtgg tcggcatcgt cgcaggactg
      781 atcgccaccg agcaggccga gctgcactac ggcgacgccg acgcccaggc ccgggtcgcg
      841 tcgacggtcc cggccggccg gctgggcacc cccgaggacc tggcccaggc atgccgctgg
      901 ctgacctcgc ccctggcgtc ctacgtgagc ggatcctcgg tctgggtcca cggcgccggc
      961 gaacgacccg cgttcctggc cgcggcccgc ggcgagtagg cccgaaccgg gctcgggctc
     1021 gggctcgggt ccgcgtcccg cgccggggtg cttcctacgc tggtcggatc gctccgctgg
     1081 ccacctccat cgcgccgtcg atccgacgag gcgtcgccgc cctcgccctg ggggtgctgg
     1141 tcgcgttcgt cctcggcggg tgctcggacc tggtccagca ggccgacgcc accgtcgaca
     1201 tcgcggcgga ggacaccaac caggccaccc agctgcgcca ggacctgttg cagcgtgcgc
     1261 cgtcgtgggg cggggtgcgc gtggccgagg acaccgacga gcagcgcggc gacatctcgc
     1321 tgaccttctc gttgccgggg cgcaacctcg acgccgccct cggcaccatc cactcgctgg
     1381 acgccgacgt cgagtcgcag tcgatcgacg tcgagcgagg cgacgtggag cgcacgacca
     1441 ccaccgccaa ggaggggacc ccgagcgccg gcgacgacgg ccaggtcacc ctgcgggtga
     1501 acatcagctc gcagcccgcg gccgccggcg cgggcgcggt gctgcgcctg gtcatggccg
     1561 tcttcagcat catcgggatg gtggcgacgg cactgtggat cagccgggcg tggaagaagc
     1621 gcttcggcgc cgaggagtcc ccgcgaccgc ggcggcgcaa catcgacgtc gacctcagcg
     1681 acccgcccac ccaggagacc ccccaggtcc cccgcggccc ctggaactga ccgacaggcg
     1741 aacccccgga tccggcccga cacgaccggg ccacccgacc cacacggcga cacgcccgct
     1801 gtggcgcccg atcggtgcgg ctcagccggt cagccccagc gcggcggaga gctcccgcca
     1861 ctccgcccgg ggcaggtgcg ccatgtcggg gtgcagcacc tggacgcaca cgtggtccgc
     1921 cccggcgtcg aggtgcgaac ggatccgacc gaggacgtcg tcgaggtcgc cgtggacgac
     1981 gatggcgtcg accaggcggt ccgagccgcc gtcggccacg tcctcgggtc cgaagccgag
     2041 ccgggccagg ttgttcgtgt agttgggggc gctcaggtac accgccatgt gctggcgggc
     2101 cgtctcccgg gcggtggccg gatcggtgtc cagcacgacc atctgctcga cggccaggag
     2161 cggctccgga cccatcgtct cgcgggcgat cgcggtgtgc tcgggcggca cgaagtaggt
     2221 gtgggcgccc gcggtccgct cggcggcgag cgagagcatg cgcggcccga gcgcggcgag
     2281 caccatctgc gccggcggat caccccgagg agcgaagaag cgggccttgg ccatcgcgtc
     2341 gaggtactcg accatccccg agtagggccg ggagtagtcg tgcttgcgca cccacgccac
     2401 caggtgctcg tgactgacgc cgagaccgag caggaaccgt cccgggaacg cctcgttcag
     2461 ggtctgcatg gcggcgttgg ccgccagcgg atcacggccg taccggttga cgatgccggt
     2521 cgcgaccttg atccgcggcg acgcggccat gagcagcgcg gcgtgcacga agggctcccg
     2581 gaacaccgtc tcggggatcc agaccgctcc gtagcccaac tcgtcgagct cggcggtcgc
     2641 ctcgcccgcc accgccgagg gcaccatgtc gagagcaccg gtccagatgc cgtaggtgcc
     2701 gatgtcgatg tgtgggttct gcacgtcgtc tccgttnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnncgcc gatcgggtca cgggttgatg tagaggaagc cgaacgcctc
     2881 gtcgcggacg tcgaggccgg cctcggccaa cgccggcgac cagtcgccgc cgacggcggg
     2941 gtcgctctcc tccatgccgt ggtccgcgca gaggaacacc gtggtctgct cgcggatgcc
     3001 gcgacgctcg agggcggcca ggacctctcc gatgcggccg tcggtgtcgc ggatggacgc
     3061 ccgggcgatc tcggagtggg gtccgccctc gtggaacgcc gagtcggtca gcgcgtagtt
     3121 gacccacagg aacgtcggca gcgggtggtc ggtgtcgagg tagcgcccgt cgacgatgcc
     3181 gaccgcctgc tcggtgccgt ggtggtcgac ctgggtgaac agggcgtagt ccttgaccgg
     3241 ccgcacgaac atctcggagc tgtgcggcag accctcggcc gagcggggga agtcccccat
     3301 ccgcacccca cgacgcatca ggtcgaacgt cgagtacacc gctccgtggt ccgccggctc
     3361 gttgaccgac gcggtgaagc agccgggccg ggcccgccgg agggcgtggt ggatgctgtc
     3421 ggttcccggc gacagggagt ccatcgcggt cgcccacgtc tcgggcgagt tggtgatcac
     3481 ctgctcgcgc cggtcgcggt cccaccaggc gttgtgcagg atcccgtggt ggccggggtg
     3541 ccgaccggtg atcaccgtcg tgtggttggg aagcgtcacc gtcggcagcg acgacacgat
     3601 gccgtcgcgc agggccgtgc cgtccgcgag caggcccgcg atgtgcggag cctccccgcg
     3661 tgcgaccgag tcgtacagca cgttcgggtt gcacccgtcg agcaggaagc ccaccacgtg
     3721 gcggggcccg tcaccccggt cgagcgcggc gcggtcgagc acggcgtcga tcggccggcc
     3781 gtcggcgccg gccagggtcg acgggtcgac gccgagcagc gcgaggacgg tgggcgcgat
     3841 gtcgaccatc tgcgcgaccg ggccggccac cagcccgtcg gagcgggcac cgggcccgct
     3901 gatcaggaac ggcgcccggc tctgaaccgc acccagggag ccgtgctcgc cgatgtggcc
     3961 gccctgctcg gcccagtggt gcgacgcggt gtgcacgacg cagaggtcgg gggcgtgcgg
     4021 gtggtcgaag agctgggcca cctgctcgaa ggcgaagggg taggcgttgg cggcgcgttc
     4081 ggggaaccgg gcggcgagct cggcgtcgag tccggcgaag cggtccgtcg cacggtcccc
     4141 gagggggtcc cggccggtca cggcgtcgac ggtgaagctc cagtcccggg ccggcccagc
     4201 agatccgtcg ggcgcggcgg ccgggtcgtg gcggcggaac cggaccgatc cgtcgaccgc
     4261 gtgggcttcg taggcgccgt cgcgtcgggt cagcaccatc tcgacgatgg acgcgagcga
     4321 cgggtcgacg agggtcgcga cggcgaggtc gagttctgcg gccgacggcg acatggaccg
     4381 acggtagccc caccccggga tataggggtg ctcactcaca acccttgtca caatcccgac
     4441 cacagaaatc cgaccgaggc gaatcgcccg aaaagacggc ttcgggcggg atcccggtcc
     4501 ctgccgagat cccgcccgaa gggggacggc ccctccccgc cgatcgaggt tcccgggtta
     4561 ccgggccgat cggcgttctc cataccgtcc cgtcgtggcg atggggcaac ctccacgact
     4621 ccgtttggaa cagttctact gtgcgatcgg acatcgggca agcgcgatcg cgatcccggt
     4681 gccgatccgg gggattcccc tatcggcatg gcgatcgggg ggtgaacgtg cagtgacccg
     4741 tcgttgactc acggtcaacg acctcgtccg cagccccaac tccgtagtga ccactacggt
     4801 gcgacgcgag cgtccggtac gctgcgacca tgtcgggatc atcgagcacc cgcgacgacc
     4861 tggtccgggc cggggtcgag ctcctggacc aactgcccct ggatcggatc ctcgccgggg
     4921 tcaccaccgc ggccgtcgct gaacacgcag gcgtcaccac cgggtcgttc ttccaccatt
     4981 tccgcaactc gtcggagtac accgcggcag tggtgcgctc cttcgaccgg gaacgccggt
     5041 tcgaacggga cgccgtcagt gacctgaccg acgccatcca gcaccgacgt ctgtccgagg
     5101 cgatcgccgc cgtcctcgtc accacgtggc gcatcctcgc ggacgacccg caacgccagg
     5161 ccgaccgccg cgggcagatg cacctgttcg cgcaccaccg ggtggagctg cccccgaacg
     5221 gcaccgaccc gacaccgtcc gggccgacgg agaccgtcgg tgacgtcctg cgcgacatct
     5281 accggagcca ggtcgaccag atcaccgcga catggcagcg actcctctcc ctcggcgaga
     5341 tgcgcctggc cgagccgttc gacacccgac gactcgcggt ggcggtccac gccctgctgc
     5401 tggggctcga gatcgtccac gccgtcgacc ccgacgccgt cgacgacaac ctcttcgccg
     5461 aagccaccgc gaccctcgct gcggccctct cgcgactcga cctcgacccc gcacgcgtga
     5521 tgaccgccgt cgagctcggc cagaccgagg cgacatctcc gcaggcccgc tccggagccc
     5581 ggcgtcggct ggacacccgt cgacgcatcg tggccgccgc caccggcatg ttcgactccg
     5641 gctgggacca cgtgagcgcc aaggagatcg gagccaccgc gggggtgtcg acccagacgg
     5701 tgatcaacct gttcggcaac gtccgccggg tgtgcgccgc caccttcgtg cgtcacctcc
     5761 ccgcggtcga ggccgcgctg ggctcgaccg gccaggaccg accacgggcc ggcgtcgcgg
     5821 ctgccctcgg agtgatcgcg gacgccgccg ccggcgaccc ccacccggcc cgggccctgc
     5881 tggccgaacg ggtcggtgtc cgagtggaac ggggcttcga gctcgacgac gacgacatcc
     5941 gggtgatcgt cccgatcgga atccgcatcg cccagcagat cacccgggtg accggcctcg
     6001 acatcgcctc cccgcacgtg cccgacctca gctcgctggt ggtcgacttc acgctcggcc
     6061 acgcggttcc ccggcccgac agccgcgacg acaccgtccg acgtgccatg gccctgctcg
     6121 acgacgccct cggcgagctc cccatcgact cggagccgcc cggcgtcccc cactactctg
     6181 acgatccgtc agatcttcgc cacgacaccc cggacgcccg ggggaacggg agactccagt
     6241 gagcaccaga cgagtgcccg cgatcgacgg ctggttcacc atgccgaccg acggctcggc
     6301 ccccgccctg atcggcaccc gcgacaccac gacgggcacc tacttcttcc cgcccgagcg
     6361 ggcgagctcc cgggcgcccg gcggagccga cgccgagctg gtcgacgtcg agctcagccg
     6421 gaccggtcgt ctctggtcct acaccgacgc gggctaccgc cccccggagc cctacgtccc
     6481 ggtgaccgac ccgcacgtcc cgttcgccat cgccgcggtc gagctcgccg aggagaagat
     6541 ggtcgtcatg ggtcaggtgg tcgccggggt cgccgtcgac gacctctccg tcggcatgga
     6601 gatggagctg gtgctcgacg tcctctactc cgagccggac cccgacggcg ccgacggcga
     6661 cgtcgtcgag cacgtggtgt ggaagtggcg accggtcggc ggcaccaacg ctgccggggc
     6721 cgtgagcgca ggagagccgt catgaccgtc gatcaggtcg ccgtcctcgg tgtcggcatg
     6781 cacccgtggg gcaagtgggg acgcaacttc gtcgagtacg gcgtggccgc ggcccgggcc
     6841 gcgctcgccg acgccggggt ggacgccgcc gacatccagt tcatcgccgg ggccgacacc
     6901 atgcgcaacg gctaccccgg ctacgtcgcc ggagccacct tcgccaaggc gctcggctgg
     6961 agcggcatcc ccgtcaccag cgcgtacggg gcctgcgctt ccgggacgat ggcgatcgaa
     7021 gccgcccgcc agcgcatcct cgccgggctg tgcgacgtcg ccctggtcgt cggcgccgac
     7081 accaccccca agggcttcct caagcccaac aagggcgagc gaggcgacga cccggactgg
     7141 ttgcggttcc acctcatggg ggcgaccaac ccgacctact tcgcgctcta cgcccgacgg
     7201 cggatggacc tgtacggcgc caccctcgac gacttcgccc aggtgaagat caagaacgcc
     7261 cgcaacggcc tgggcaaccc ctacgcgcgc taccgcaaag aggtgaccgt cgcggacgtc
     7321 gaggcgtcgc ccacggtggc cagcccgctg cggctgctcg acatctgcgc cacctccgac
     7381 ggcggcgcgg cgatcgtgct cagctcgatg gactacgccc gacgcctgcg cgacggctcg
     7441 gatccgacgg tgcggatcgc cgcggtgtcg acggtgacgc cgacgttccc caacacgatc
     7501 atcgagatgc cggacttcgc caccgactcc gccgcgggca ttgcggcccc ggagctgacc
     7561 ttcaagcagg cgatcggcgc ggcggcctac gagcaggccg gtctcggtcc cgacgacgtc
     7621 gacgtggccg aggtctacga cctgtcgacc gcactcgagc tcgactggta cgaggacctc
     7681 gggttctgcg gaccgggcga ggccgagaag ctgctccgcg acggcgtcac ccagctcgac
     7741 ggccgtctgc cggtcaaccc gtccggtggg ctcgcctgct tcggcgaggc ggtgccggca
     7801 caggccatcg cccaggtgtg cgagatcacc tggcagctgc gcggtcaggc cgagaggcgc
     7861 caggtcgcgg accccaccgt cggcatcacg gcgaaccagg gcctgttcgg ccacggatcc
     7921 agcgtcatcc tcacccgctg acccgttctg tagtgcgaga tccaggcgtt cgcctggatc
     7981 tcgcacgaca gaacgcctgg ggccgggcgg gaacgggacg agccgcaccg agccgagggc
     8041 tcagttccgc gcggcagcgg ccgatggaac ggtgggaaca ccacacggca cacgacaccc
     8101 ggggccgcac ggccccggtg tcgtggacct caggccacac cagctcagcg catcccccgg
     8161 cgggcaccca ccagcgcggc caccgcgatc gccgcccccg ccagcacgag cagccccgcc
     8221 ggcacaccga tcgccccgga caccgctccg accaggccgg gcccgatgac cgagcccagg
     8281 tagccgaacg tggtcaccgc cccgaccagc acgcccgggc gctcccgacc ccgaccgacc
     8341 tcggacatca gcagcggcca gcacaccgac accccacccg cggccaggac caggccggcc
     8401 gccgcgagga ccggctggct caccgtcgcc agcaccacca gtcccaccgc cgcgagcacg
     8461 gtgccgccga tcaccgcccg gcgagccgcc gccgcggtct cgaccctcga caccgacagc
     8521 cgcgccgtca ccgccaccag gtagccgacg aacgccccgc ccgccccgac gagtaggccc
     8581 gcgtcgagct gacctcgcag ctgcaacacc ccccaggtgt tcacccctcc ctcgaccacg
     8641 gccgccaacg cgagcgccac cgcgaccggc accagccgct gggagcgcag ctcgcgcatc
     8701 ccgtcggaga accgcaccgc cgatccggca ccccccgccg gcagatggac ccccgcggtg
     8761 acgaccacca cgccgacgac caggacggcg accaccaccc aggcccaccg ccatccggca
     8821 gcaccggcca cggccaggag cacgccgacg acgacggtgc ccgcggcgcc ccccaggttg
     8881 aacaccgcgt ggagccgcac caggtgtccc ggacggtcgg ccagaccggc ggtcgccgcc
     8941 acgttggcga tgacgtcgac ggccccgccg agcgacagca cgaccacgat gccggtggcc
     9001 accccccacg gcgccg
//
LOCUS       DAOQOD010000129         8972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2866_length_8972_cov_11.255579, whole genome shotgun sequence.
ACCESSION   DAOQOD010000129 DAOQOD010000000
VERSION     DAOQOD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_8972_cov_11.255579"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLZ72_11840"
     CDS             <1..1292
                     /locus_tag="PLZ72_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37034.1"
                     /translation="ADSRSVRTVGERSARHIAMTTTSSKTRTSSGRAGSGRWALCAAV
                     TEEMLNDLMVFAVGEGVSLDPVTTDVALPAMGDVRLKVVLTVTGGTFDLRADDGGRAR
                     VVVDATGVVDVAAVDFEGDSAPVSPMLPSGPIPIPIQVAALVDPVVELRDDLTVRAGL
                     DVGGGELIGVSVPDDTAAPDGIDPAAWMPMTQVFNMLFSSMGTQLWESLGEHVGVVGS
                     ELGSDVGEILADLGVAVGRAEVSVGSGLLTVGLPATEDVIGRATPSPVAGKRVAVSIA
                     QSGVHRLAALLIPLALGPRPLPFEIEVDLGEQSVRGRLRQTRILSERIPDLRMSLRTD
                     IRPRLTGGRLELSLRAAWVELPSLFGGFGLTGAINDMSRRVGGLASFAPLNLRFPAVI
                     PVPLGTTGDDGTPDTLGVRIDDLRVTGDGVGVVAGLA"
     gene            1289..2098
                     /locus_tag="PLZ72_11845"
     CDS             1289..2098
                     /locus_tag="PLZ72_11845"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNA37035.1"
                     /translation="MNPEPAPAQNGVDLPHSHLRPGLARDGIVNLRDLGGTPAADGAV
                     IAPRTLIRADALQRCSPASVRALYDHGVRRVLDLRDDAERVESGSFTVVDDLPIDVQH
                     VPVVDPAYVWEIDGVALEQVLAHRYADILGAFGDRFAAAVELVASTDGAAAYHCAVGK
                     DRTGLLTVLLLGALGAPREAIVADYVRSARVNVMQVARLRVLGHPYGAATDADLAVGV
                     WSARASTIESTLDLLDDEHGGAVRYLRDAGVDDEVVASLRHRVLIRPDRTG"
     gene            2127..2582
                     /locus_tag="PLZ72_11850"
     CDS             2127..2582
                     /locus_tag="PLZ72_11850"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNA37036.1"
                     /translation="MLDQGDIAPEFTLSDQAGVDRSLSELLADGPVVLFFYPRAMTAG
                     CTRESCHFRDLAAEFEAVGAQRVGISADDVDRQARFDERNTLGFPLLSDPSRTVAAEF
                     GVKRPLALMNKRSTFVIDTDRRILAAISSELNMDLHADEALRVLRTRES"
     gene            2585..5833
                     /locus_tag="PLZ72_11855"
     CDS             2585..5833
                     /locus_tag="PLZ72_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNA37037.1"
                     /translation="MAPETSSPQPVLRPEWWRDPQLTSWNRLPARPPLVPFADVAGAR
                     SHDPGVSPWWRSLDGKWGFTGFDRPEDVTAESLRGPDPDHGVGPSHMTVPGAWTLQGF
                     SSPHYTNVVMPFPGDLPEVPEANPTGVYATTVTVPREWRGRRVVLRVGAAESVVVAFV
                     DGVAVGLGTDSRLPSEFDITSHVRAGRRATIALAVVRFSAQTWVEDQDQWWHGGLQRS
                     VTLHSTAPDHLARVELVPGLDGGGESGGDGGAVGTLAVRVDVDGPMTSVPVAGTRARD
                     GVEDPGPGTVEVHVETLRGRRLATTGPIEVPRWNDTAEVTRLVSAMFVRPGHVEAAVR
                     VPGISPWSAEDPQRYRAVVVLRDGHGDVVEVTAQQTGFRSVEVRDRQLLVNGEPVMIH
                     GVNHHEHDPRRGRAVSRDLTRRDLELMKAHNINAVRASHYPHDEHFAELCDELGLYVV
                     DEADIESHGRQESLVHDPRYAATIVERVERMVRRDLNHPSIIVWSLGNESGYGAPHDA
                     AAAFVRRHDPSRPVQYEGPFIRDLDAEAPVSDIVCPMYPAIDDIVARAGRAGDDPRPL
                     ILCEYSHAMGNSNGGLTDYWDAFRATPGLQGGFIWEWVDHGIPLPRRSVPLPGAGGSD
                     APERAPVFGYGGDFGDTPHDGNFVCDGLVGADRVPRPAMAEVAHVGRPVHLEVLDAAR
                     GRVRLTNVAHFTDTSGLVARWELLLDGTVARRGELAVEPIGPGRSRTVRIPLPRSLPV
                     DAAEAFVTLRWHLRRATPWARAGHVVATEQFEVPTRAVGSVSASGATRSRRRQLNAGT
                     DVRSEPPVDLVWTPTLSRALTDNDAIQLSWMREFSERLARWDSLGVLDASWRPGPIRT
                     RSRSGAQVVTSTGELVPNGAAPAVAVRMRELVGAGDGWVHLAVTFSLPPELADVPRIG
                     ITTVLPGSFDDLEWYGDGPHESYSDRRGSVTVGRWRSSVAEQYVPYVFPQEHGFHTGL
                     RWLALRETARGRGPRTGLLVVADGDLGFSARHHSDADLFAATHTDELPHDRALTHLAL
                     DVGQRGLGTGSCGPDTAEAHRLRAGSHRLAVWLRFFDARREDPAALARLATG"
     gene            complement(5939..7237)
                     /locus_tag="PLZ72_11860"
     CDS             complement(5939..7237)
                     /locus_tag="PLZ72_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNA37038.1"
                     /translation="MPVTEVRSPAEAAALIRPADTVAIGLGPAHPTALLHALGTRDDW
                     VRLEMFGALLTDLYEVFTRPGVRLRSGFFGPAERFLRDSGANVEYVPADFRRFAPIIE
                     RLRPRVMATAAAPPDADGFVSLSLHAGATVEALHATGADPDRVLIVEVSPNYPRTFGL
                     DPHHHRLHLDEIDVLVESNGAPVDLADAPGTDVDRAIAETAMRFISDGATLQTGIGAV
                     PSLIATALAEGPGGGYGIHSEMFTTGLMRLHRAGKVTNENKGTYPGVSITTFAAGTRE
                     LYDWLDGNDVVRFLPVHVVNDARVIAANDHFVSINGATAIDLHGQVAADTIRGQQHSG
                     TGGHEDFLAGAGLQADDRSLLCLRSSVVIDGERVSRVRVDVGPDMLVTTPRHQVDVVI
                     TEHGAAELSGHTVRERAFALAAIAHPDFRDELNEAADRMA"
     gene            7279..>8972
                     /locus_tag="PLZ72_11865"
     CDS             7279..>8972
                     /locus_tag="PLZ72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA37039.1"
                     /translation="MNHADGRATETGPTIAMPEIDIATAWEAIADEIPDADALIHGDV
                     VRSWADFESRAARVAGHLVAAGLGPDDKVAFYLHNGTEYLEATFGAFKARCVPVNVNY
                     RYLEAELAYLLDNSDAAAVVVSAELVDRLGAVIGQLPAVRTVLVVGATDEHPVPDGSS
                     APWDLAAGVDDYDSAVAAAEPMARIARSADDLWFLYTGGTTGMPKGVMWPHRSLIGTS
                     AATFRVIRSEVPSTAEGFAAAARTFHDTGRAIRLLAAAPLMHGTSGIAANGVLLSGGC
                     VTTLSGRSFDADELCRTVQERRITQLTIVGDAFAKPIVDALEAADAAGRPYDLSSLRM
                     IVSSGVIWSRPVKDALLGWTDAILADLLGSSEGTGFGTSVSRRGSSAGTAAFRLGEHA
                     RVFTDDGVEVEPGSGQVGVLAVGGPIPIGYYKDAAKTEATFRTFAGRVWSVPGDFATV
                     EADGAITLLGRGSVCINTAGEKVYPEEVEEALKLHPSVHDANVVGLPDDRWGQAVTGV
                     VELVGGAEDSEDLRAEIVASTKEHLAGYKCPKQLVVVERIRRGPNGKADYRWATDTA"
BASE COUNT         1162 a         3262 c         3265 g         1283 t
ORIGIN      
        1 gtgccgactc ccggtcggtg cggaccgtcg gggagcggtc ggctagacac atcgccatga
       61 ccacgacgtc gtcgaagacc cgcacctcgt cgggccgggc cggatcgggc cgctgggcgc
      121 tgtgcgccgc ggtcaccgag gagatgctga acgacctgat ggtgttcgcc gtcggggagg
      181 gggtctcgct cgatccggtg accaccgacg tggcgttgcc cgccatgggc gacgtgcgcc
      241 tcaaggtcgt gctcaccgtc accggcggaa ccttcgacct gcgcgccgac gacggcggcc
      301 gggcccgggt ggtggtcgac gccaccgggg tggtcgacgt cgccgcggtc gacttcgagg
      361 gtgacagcgc acccgtgtct cccatgttgc cgtcggggcc gatccccatt cccatccagg
      421 tcgccgcgct ggtcgacccg gtggtcgagc tgcgcgacga cctgaccgtg cgcgccgggc
      481 tcgacgtcgg cggtggcgag ctgatcgggg tgtcggtccc cgacgacacc gccgcgcccg
      541 acgggatcga cccggctgcg tggatgccga tgacccaggt gttcaacatg ctgttctcct
      601 cgatggggac ccagctgtgg gagtcgctcg gcgagcacgt cggcgtggtc ggcagcgagc
      661 tcggatcaga cgtcggagag atcctcgccg acctcggcgt ggcggtcggt cgggccgagg
      721 tgagcgtcgg gtccggtctg ctcaccgtcg gcctgcccgc caccgaggac gtcatcggcc
      781 gagccacgcc gtcgccggtg gccggcaagc gcgtcgcggt gtcgatcgcc cagtccgggg
      841 tgcaccggct ggccgcgctg ctcatcccac tggccctggg cccccgcccc ctgccgttcg
      901 agatcgaggt ggacctgggg gagcagtcgg tcaggggtcg cctgcgccag acccgcatcc
      961 tgtccgagcg catccccgac ctgcggatgt cgctgcgcac cgacatccgc ccccgtctca
     1021 ccggcggccg cctggagctg tcgctgcgcg ccgcgtgggt cgagctcccg tcgctgttcg
     1081 gcgggttcgg gctgaccggg gcgatcaacg acatgtcgcg ccgggtgggc gggctggcgt
     1141 cgttcgcccc gctgaacctg cgcttcccgg cggtgatccc cgtgccactg ggcaccaccg
     1201 gcgacgacgg aacgcccgac accctgggcg tgcgcatcga cgacctgcgc gtcaccggcg
     1261 acggcgtcgg cgtggtcgcg ggcctggcgt gaacccggag ccggccccgg ctcagaacgg
     1321 ggtcgacctg ccccactcgc acctgcgccc gggcctggcc cgcgacggga tcgtcaacct
     1381 gcgcgacctg ggcggcaccc cggccgccga cggtgccgtc atcgcccccc gcaccctgat
     1441 ccgtgccgac gcgctgcagc gctgctcgcc ggccagcgtg cgggccctct acgaccacgg
     1501 cgtgcgtcgg gtgctcgacc tgcgcgacga cgccgaacgg gtcgagtccg gcagcttcac
     1561 cgtggtcgac gacctgccga tcgacgtcca gcacgtgccg gtcgtcgacc ccgcctacgt
     1621 gtgggagatc gacggcgtcg cgctcgagca ggtgctcgcc caccgctacg ccgacatcct
     1681 cggcgcgttc ggtgaccgct tcgccgccgc ggtcgagctg gtcgcctcga ccgacggcgc
     1741 cgccgcctac cactgcgcgg tgggcaagga ccgcaccgga ctgctcaccg tgctgctgct
     1801 cggggcgctc ggtgcccccc gcgaggcgat cgtcgccgat tacgtccgct cggcgcgggt
     1861 caacgtcatg caggtggcgc gcctgcgggt gctgggccac ccctacggcg ccgccaccga
     1921 cgcggacctg gcggtcgggg tgtggtcggc gagggcctcc accatcgaga gcacgctcga
     1981 cctgctcgac gacgagcacg gcggagcggt ccgctacctg cgcgacgcgg gcgtcgacga
     2041 cgaggtcgtg gcatcgttgc gccaccgggt gctgatccga ccggaccgca ccgggtagcc
     2101 accggaccga ccaccgagga ggccgaatgc tcgaccaggg cgacatcgca ccggagttca
     2161 ccctgtcgga ccaggccggc gtcgaccgca gcctgtcgga gctgctcgcg gacggtcccg
     2221 tagtgttgtt cttctacccg cgggccatga cggcgggctg cacccgcgag tcgtgtcact
     2281 tccgggacct ggccgccgag ttcgaggcgg tgggcgccca gcgtgtcggg atctccgccg
     2341 atgacgtcga tcgtcaggcc cgcttcgacg agcgcaacac cctcggcttc cccctcctgt
     2401 cggatccgtc gcgcaccgtc gccgccgagt tcggggtgaa gcgaccgctg gcgctgatga
     2461 acaagcggtc caccttcgtg atcgacaccg accggcggat cctcgccgcc atctcctcgg
     2521 agctcaacat ggacctgcac gcggacgagg cattgcgggt gctgcgaacc cgggagagct
     2581 gaccgtggcc ccggagacgt cgtcgcccca gccggtcctg cgacccgagt ggtggcgaga
     2641 cccccagctg acgtcgtgga accgcctgcc ggcgcgaccg ccgctggttc ccttcgcgga
     2701 cgtcgccggc gcccgctccc atgaccccgg ggtcagcccg tggtggcggt cgctcgacgg
     2761 caagtggggc ttcaccggct tcgaccgacc cgaggacgtg accgccgagt cgctgcgggg
     2821 cccggacccc gaccacggtg tcggcccgtc gcacatgacg gtgccgggcg cgtggacgct
     2881 gcagggcttc tcgtcgccgc actacaccaa cgtcgtcatg ccgttccccg gcgaccttcc
     2941 cgaggtgccc gaggccaacc cgaccggcgt gtacgcgacg acggtgaccg tcccgaggga
     3001 gtggcggggc cgacgggtcg tcctgcgggt gggcgcggcg gagtcggtgg tcgtcgcctt
     3061 cgtcgacggc gtcgcggtcg gcctgggcac cgacagccgc ctgcccagcg agttcgacat
     3121 cacctctcac gtgcgggcgg gtcggcgcgc cacgatcgcc ctggccgtcg tgcgcttctc
     3181 ggcacagacg tgggtcgagg accaggacca gtggtggcac ggcggcctcc agcgcagtgt
     3241 caccctgcac tccaccgccc cggaccacct ggcccgcgtc gagctcgtcc ccggcctgga
     3301 cggcggaggg gaaagcggag gggacggcgg cgcggtcggc acgctggcgg tgcgcgtcga
     3361 cgtcgacggt ccgatgacgt cggtccccgt cgccggaacc cgtgcgcggg atggtgtgga
     3421 ggatccggga ccgggcaccg tcgaggtcca cgtcgagacc ctgcggggcc gccgcctggc
     3481 cacgaccggg ccgatcgagg tcccccgctg gaacgacacc gccgaggtca cccgcctcgt
     3541 gtcggccatg ttcgtcaggc cgggtcacgt cgaggcagct gtccgggtgc cggggatctc
     3601 gccctggtcg gcggaggatc cgcagcggta ccgggccgtc gtcgtgctgc gcgacggcca
     3661 cggcgacgtc gtcgaggtca ccgcgcagca gaccggcttc cgctcggtcg aggtgcgtga
     3721 ccggcaactg ctggtcaacg gcgaaccggt gatgatccac ggcgtcaacc accacgagca
     3781 cgacccgcgc cgcggccggg cggtgagccg cgatctgacc cgtcgcgacc tggagttgat
     3841 gaaggcccac aacatcaacg cggtgcgggc ctcgcactac ccccacgacg agcacttcgc
     3901 ggagctgtgc gacgagctcg gcctgtacgt ggtcgacgag gccgacatcg agtcccacgg
     3961 ccgtcaggag tcgctcgtcc acgacccccg ttacgcggcg acgatcgtcg agcgggtcga
     4021 gcggatggtg cgccgggacc tgaaccaccc gagcatcatc gtgtggtcgc tcggcaacga
     4081 gtccggctac ggagcaccgc acgacgcggc cgccgcgttc gtccgccgcc acgacccgtc
     4141 ccgaccggtg cagtacgagg ggccgttcat tcgcgacctc gacgccgagg ctccggtgag
     4201 cgacatcgtc tgtccgatgt accccgccat cgacgacatc gtcgcccggg cgggccgggc
     4261 cggagacgac ccccgcccgc tcatcctgtg cgagtactcc cacgccatgg gcaactccaa
     4321 cggcggcctc accgactact gggacgcgtt ccgggccacg cccgggctgc agggcggctt
     4381 catctgggag tgggtcgacc acgggatccc gttgccgcgc cggtcggtac ccctcccggg
     4441 ggcgggcggg tcggacgcgc cggagcgggc tccggtgttc ggctacggcg gcgacttcgg
     4501 cgacaccccc cacgacggca acttcgtctg cgacggcctg gtcggcgccg atcgggtccc
     4561 gcgcccggcg atggccgagg tcgcccacgt cggacgcccg gtgcacctcg aggtcctcga
     4621 cgccgcccgc ggtcgggtcc ggctcaccaa cgtggcgcac ttcaccgaca cgtcggggct
     4681 ggtggcccga tgggagctgc tgctcgacgg caccgtcgcc cgccgcggtg agctggccgt
     4741 cgagccgatc ggccccggac gctcccgcac cgttcggatc ccgctgccgc gttcgctgcc
     4801 ggtcgacgcc gccgaggcgt tcgtgacgct gcgatggcac ctgcggcgcg ccacgccgtg
     4861 ggcgcgggcc ggtcacgtcg tggccaccga gcagttcgag gtgccgaccc gggcggtcgg
     4921 ctccgtctcc gcgtcgggag cgacccggtc gcgtcgacgc cagctgaacg ccgggaccga
     4981 cgtccggtcc gaaccgccgg tcgacctggt ctggacgccg acgctcagcc gggccctcac
     5041 cgacaacgac gccatccagc tcagctggat gcgggagttc tccgagcgac tggcgcgctg
     5101 ggactcgctc ggggtcctcg acgcgtcgtg gcggcccggc ccgatccgga cccggagccg
     5161 gtcaggcgcg caggtggtca cctcgacagg ggagctggtc ccgaacggcg ccgcaccggc
     5221 ggtcgcggtg cggatgcggg agctggtcgg cgccggcgac gggtgggtgc acctggcggt
     5281 gaccttctcg ctgccgcccg agctcgccga cgtgccccgg atcggcatca cgacggtcct
     5341 gcccggatcg ttcgacgacc tcgagtggta cggcgacggg ccccacgagt cctactcgga
     5401 ccgtcggggg tcggtgacgg tcggccggtg gcgatcgtcg gtcgccgagc agtacgtgcc
     5461 gtacgtgttc ccccaggagc acggcttcca caccggactg cgttggttgg cgctgcgaga
     5521 gacggcacgg ggccggggtc cgcgcacggg gttgctggtc gtcgccgacg gcgacctggg
     5581 cttctcggcc cggcaccact ccgacgcgga cctgttcgcc gccacccaca ccgacgagct
     5641 cccgcacgac cgtgcgctca cccacctggc gctcgacgtc ggccagcggg gtctggggac
     5701 cgggtcgtgc gggccggaca cggctgaggc tcaccggctg cgggccgggt cccaccggct
     5761 ggcggtgtgg ttgcggttct tcgacgcccg ccgcgaggac cccgccgcgc tggcccgcct
     5821 ggccaccggc tgacccccgt tctgccgact tcccgttctg tagtgcgaga tacaggcgaa
     5881 cgcctggatc tcgcactaca gaacggtcgg ggagggggtt cggcgggagg ggagagtgtc
     5941 aggccatgcg gtcggcggcc tcgttcagct cgtcgcggaa gtccgggtgg gcgatcgccg
     6001 ccaacgcgaa cgcccgctcg cgcacggtgt gacccgagag ctccgctgcg ccgtgctcgg
     6061 tgatgaccac gtcgacctgg tggcgggggg tggtgaccag catgtcgggg ccgacgtcga
     6121 cgcggaccct cgacacccgt tcgccgtcga ttaccacgct ggagcgaagg cacaacagcg
     6181 atcggtcgtc ggcctgcagg cctgctccgg ccaggaagtc ctcgtggccg ccggtgccgg
     6241 agtgctgctg tccccggatg gtgtccgccg cgacctgacc gtgcagatcg atggcggtgg
     6301 cgccgttgat cgacacgaag tggtcgttgg ccgcgatcac ccgggcgtcg ttgaccacgt
     6361 gcaccggcag gaaccgcacc acgtcgttgc catcgagcca gtcgtagagc tcccgggtcc
     6421 cggccgcgaa cgtggtgatc gacacgcccg ggtacgtccc cttgttctcg ttggtgacct
     6481 tgccggcccg gtgcaggcgc atcagcccgg tggtgaacat ctcggagtgg atcccgtaac
     6541 cgccgccggg accctcggcc agcgcggtgg cgatgagcga cgggaccgca ccgatgccgg
     6601 tctgcaacgt cgctccgtca gaaatgaagc gcatggcggt ctcggcgatc gcccggtcga
     6661 cgtcggtgcc cggcgcatcg gccaggtcga ccggcgcgcc gttcgactcg accagcacgt
     6721 cgatctcgtc gaggtggagt cggtggtggt gcgggtcgag gccgaacgtg cgggggtagt
     6781 tgggcgagac ctcgacgatc agcacccggt cggggtcagc gccggtggcg tgcagcgcct
     6841 cgacggtggc gccggcgtga agcgacaggc tcacgaagcc gtccgcgtcg ggcggtgcgg
     6901 ccgcggtggc catgacccgt ggtcggagcc gttcgatgat cggggcgaag cgccggaagt
     6961 cggccgggac gtactcgacg ttcgccccgc tgtcgcgcag gaaccgctcg gctggtccga
     7021 agaacccgct gcgtagccgg acccccggcc gggtgaacac ctcgtagagg tcggtcagca
     7081 gcgcgccgaa catctcgagg cgcacccagt cgtcgcgggt tccgagcgcg tgcagcagcg
     7141 cggtcgggtg cgccggtccc agcccgatgg ccacggtgtc cgcggggcgg atcagggcgg
     7201 cggcctcggc tggcgaacgg acctcggtca cgggcatggt ggactcctct cgacccgggg
     7261 ggaccggagc atgctgtcat gaaccacgcc gacggtcgcg cgaccgagac cggtccgacg
     7321 atcgcgatgc cggagatcga catcgcgacg gcgtgggagg cgatcgcgga cgagatcccc
     7381 gacgccgacg ccctgatcca cggcgacgtc gtgcgcagct gggccgactt cgagtcgcgc
     7441 gccgcccggg tggccgggca cctggtcgcc gccggcctgg gccccgacga caaggtcgcg
     7501 ttctacctgc acaacggcac cgagtacctc gaggccacct tcggggcgtt caaggcccgc
     7561 tgcgtgccgg tcaacgtcaa ctaccgctac ctcgaggccg agctcgccta cctgctcgac
     7621 aactccgacg ctgccgcggt cgtcgtgtcg gccgagctcg tcgaccggct cggggccgtg
     7681 atcggccagc tcccggcggt tcgcaccgtg ctggtggtcg gagccaccga cgagcacccc
     7741 gtccccgacg ggtcctcggc cccgtgggac ctggccgccg gcgtcgacga ctacgactca
     7801 gccgtggccg ccgccgaacc gatggcccgc atcgcccgct ccgccgatga cctgtggttc
     7861 ctctacaccg gcggcaccac cggcatgccc aagggcgtga tgtggccgca ccggagcctg
     7921 atcggcacgt cggccgcgac gttccgggtg atccgctccg aggtcccctc caccgccgag
     7981 gggttcgcgg cggcagcccg gaccttccac gacaccggtc gggcgatccg cctgctggcc
     8041 gccgcgccgc tcatgcacgg cacctcgggc atcgccgcca acggcgtgct gctctcgggc
     8101 ggctgcgtca cgacgctgag cggacgttcc ttcgacgcgg acgagctgtg ccggaccgtg
     8161 caggagcggc gcatcaccca gctgacgatc gtcggcgacg cgttcgccaa gccgatcgtc
     8221 gacgccctcg aggccgcgga tgccgccggg cggccctacg acctgtcgtc gctgcggatg
     8281 atcgtcagct ccggcgtcat ctggtcgcgg ccggtcaagg acgccctgtt gggttggacc
     8341 gacgccatcc tcgccgacct gctcggctcc tcggagggga ccgggttcgg tacctcggtc
     8401 agccgtcggg ggtcctccgc cggcaccgcc gcgttccgcc tgggcgagca cgcccgggtc
     8461 ttcaccgacg acggggtcga ggtcgaaccc ggctccggtc aggtcggcgt gctggccgtc
     8521 ggcggtccga tcccgatcgg ctactacaag gacgcggcca agaccgaggc caccttccgg
     8581 accttcgccg gtcgggtgtg gtcggtgccc ggcgacttcg ccaccgtcga ggccgacgga
     8641 gcgatcaccc tgttgggccg tgggtcggtg tgcatcaaca cggcgggcga gaaggtctac
     8701 ccggaggagg tggaggaggc gctgaagctg cacccgtcgg tgcacgacgc caacgtggtc
     8761 ggactccccg atgaccgctg gggacaggcc gttacggggg tggtcgagct ggtcggtggt
     8821 gccgaggact ccgaggacct gcgcgccgag atcgtcgcgt cgaccaagga gcacctggcc
     8881 ggctacaagt gccccaagca gctggtggtc gtcgagcgga tccggcgggg cccgaacggc
     8941 aaggccgact accgctgggc gaccgacacc gc
//
LOCUS       DAOQOD010000130         8732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_2984_length_8732_cov_11.008528, whole genome shotgun sequence.
ACCESSION   DAOQOD010000130 DAOQOD010000000
VERSION     DAOQOD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_8732_cov_11.008528"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLZ72_11870"
     CDS             <1..776
                     /locus_tag="PLZ72_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNA37040.1"
                     /translation="AYQDGEEWVINGHKWFISNAHRANFAILIARTEDDPDLPQAANT
                     AFLVDIPSEGWTEVRQIETMHGSTGHSEILIEDLRVHESQMLGGRGQGHLLGQYRLGP
                     ARLAHCMRWIAQAETALDMMVDRSLNRFAHGSLLAEKQGIQWMIADSTMELYQAKLMV
                     LHAAYRIDNGLEFKSEVSMAKHFVANMLNRVIDRSIQVHGALGYSTDTPLAHMYQHAR
                     WARFADGADEVHQMRIAQRTIAAYKDSGSTASATGDLPI"
     gene            961..2391
                     /locus_tag="PLZ72_11875"
     CDS             961..2391
                     /locus_tag="PLZ72_11875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNA37041.1"
                     /translation="MSTPTVRTHQLRTGRAGRGATTDLGAFVAALIGDDPPVRIELFD
                     GSALGPPRSDVPATVQVRSRDALRRMVASPGELGLARAYVAGDIDIDGDIYAVLGLRD
                     RLPRVRLTPRLWIDAVRLLRPSDLVPLPPPPEEARPGAVAELRSRLGRGHTRETDAAS
                     IHHHYDVSNDFYELVLGPAMVYSCALWDEDRKRRGAEALEPAQDAKIELICQKLALRP
                     GLRLLDIGCGWGSLVRHAARHHGVHAVGITISAEQAAWARARVEADGLADRVEIRVQD
                     YRDIHDGPFDAVSSVGMFEHVGLARLAEYFEQVHALTAPRGRFVNHAISRPPGHAPAI
                     ARRSFVQRYVFPDGELHEVGSVITAMQAAGFEARHMEDLREHYALTLRAWVANLEQNW
                     DRAVADVGVNRARVWRLYMAGSAVGFEQGRIGINQVLGLRDPDQAHFPLRPDWTADPA
                     PPARSVVRDPHERMDVALQNTVGRDR"
     gene            complement(2405..3421)
                     /gene="ligD"
                     /locus_tag="PLZ72_11880"
     CDS             complement(2405..3421)
                     /gene="ligD"
                     /locus_tag="PLZ72_11880"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNA37042.1"
                     /translation="MARSEAVEVEVAGRSVRVTSPDKVFFPDPGVTKAGLVDYYLTVG
                     EPVMRAMGGRPVLMQRFPDGVDGNNFFQKRVPASRPDWLETTIVSTPNGTRSEALVIA
                     DLAHVVWAVNLGCLGFHVWPSLAADPEHVDELRIDLDPSPGIRFEHIRSAAFALKDLL
                     DELGLVGYPKTTGNRGLHVYLGLEPRWGAIEVRAAAVAVARELARRRPDLITDAWWKE
                     ERGERVFVDFNQNAPHKTVFGAWSVRARSMAPVSTPLSWDEVADVHPDELTVATVPGL
                     VAERGDPWEPMRTTPQSLEPLLALAEADRERGLMDAPWPPVYPKMPGEPPRVAPSRAR
                     KPTD"
     gene            3489..4793
                     /locus_tag="PLZ72_11885"
     CDS             3489..4793
                     /locus_tag="PLZ72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA37043.1"
                     /translation="MRSGRGSRSARGARGAVVGLVAGVAVLATACGAVKPPPVLNALR
                     VEGGPAAADQPRCAETPEGTEPDRVDPAEAGLDPALFRSALDYAVAKGSQSVRVYRHG
                     CLIGTGSNDPSVDWVPLPGWSATKGVVSMLAGRAVTLGRLDIDAPIGGYLNVRDPRKA
                     ALTARQLLNQTTGLRMAWVSDLNEAATTDSVAALMDRPFEAVPGTTFDYAQTTVTALV
                     AVVEAAVGEDLQSFAQRELFGPIGVGSDEWTWERDGAGRTQGFAFLSMTPTAFGRLGR
                     LLLQNGAWDGRRLISADYIAQGRAGTAANPCYGFLWRNNDGRGCHQTGPLLGLETTGN
                     WMPTVPEDAYGLSGMFDQLVLVIPSLDMVVVRLGLPHQVMADPWGDIGGEKPAMTWRF
                     FRTLMASVTDTVVADPGEWQPSTPGSIDWAHIFAVPLPEPTW"
     gene            complement(4773..5243)
                     /locus_tag="PLZ72_11890"
     CDS             complement(4773..5243)
                     /locus_tag="PLZ72_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37044.1"
                     /translation="MERRTALWLSHHWPEDYDRCVRIGHRHVCRRCLWFWPACLATMM
                     LTLAGLRWPDALDPWLVALLPVPVVIEWWGEHLGLIRYSATRQIPLTLLAAPAVGVGL
                     ARYLEHPGDRLFWGVVASYAVLCLVPFLIGPRHTRDRTPPCDADPDLRSPGRLG"
     gene            complement(5321..6427)
                     /locus_tag="PLZ72_11895"
     CDS             complement(5321..6427)
                     /locus_tag="PLZ72_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA37045.1"
                     /translation="MLSDDFMPPTADGTAARRFEPRYRVTMAHRAARSLLAAPAPVLD
                     RIIGAPPIVLDGRVLDRRVQALLAIGERLMPGDGSRSFDPDQRRREMDRMATTGMPSR
                     QGLHVSERRIPGPESTLAVRVYRPFGLEPDPPALLYIHGGGWVVGSLDAYDGTCRLVA
                     AEARCVVVSVDYRLAPEHPFPAPLDDSLAAYHWVRTHARELGIDGRRVGVMGDSAGGN
                     LAAVLAQVTLDDPVGPPQVQALVYPATEASFSKPSHELFAEGFGLTRVDMEWYRAQYL
                     PDRSTWDLPTVSPLLAPSVEGVAPAVVATAGFDPLRDEGSAYAERLSDAGVPVWYRCY
                     DDMIHGFFGMGVIPECLGMATEIARATARPLHQT"
     gene            complement(6504..8162)
                     /locus_tag="PLZ72_11900"
     CDS             complement(6504..8162)
                     /locus_tag="PLZ72_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37046.1"
                     /translation="MVDVVIRGGTVIDGTGSPGFPADVLVRDGVIAEVGTDVSAPTGV
                     ETIDATDRVVTPGFVDVHTHYDAQVFWDPDLTPSLHHGVTTVVAGNCGFSIAPIGADG
                     VGLLARTLQHVEDMSLDTLTVGVPWDEFETFGEYLDAVERRSPALNFGCYIGHTALRL
                     WAMGDDAYERDPTADELTRMRDAVAEAIAAGAIGFATSASPTHNGDRGRPVPSRRADL
                     DELRTLMAPLRDAGRGVVAMLPGGVFTNQQVFDLQQEIGRPFTWTALLTIKGLPYHEG
                     VIAEHDEARARGVDVWPQVSCRPLVFQMNLAEPFTLNTRDSFRELMDRSRDERLAAYR
                     DPQWRERARRDLDGEGFIPFNYASLAVAESDRHPELVGRGVLDVAVERGCSPLDVLVD
                     LSLEDDLRTRFWSVLANDDPDAIEWLLPRDGVLLGLADSGAHVSQLCDACFSTDLLGN
                     WVRDRGVMPIERAVHKLTAEPAAVFGLADRGVLARGRAADVVVFDPATVAPGPLRRVV
                     DFPADGERLTADQPVGVSDVLVNGVAVRRDGEPTGARPGTVLRT"
     gene            complement(8190..>8732)
                     /locus_tag="PLZ72_11905"
     CDS             complement(8190..>8732)
                     /locus_tag="PLZ72_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37047.1"
                     /translation="ACTEPLGQYQNNNIMMTNAVICLNDRARAREIALSRGRGYLNSM
                     VALYHDTMPKREGAPVWPEPPFSIPDEEVLDQLIAGGWMLCGTPEEVLEQVNNYQSVG
                     VDQLVFGFPPEGVTHEENLEMIELFGTQVIPEFDKDPVHSTTRMREAARPKYERWNQP
                     FSDVVLNAEPVIPTSALIQY"
BASE COUNT         1288 a         3086 c         3121 g         1237 t
ORIGIN      
        1 acgcctacca ggacggcgag gagtgggtga tcaacggcca caagtggttc atctcgaacg
       61 cccaccgggc caacttcgcc atcctgatcg cccgcaccga ggacgacccc gacctgcccc
      121 aggcggccaa caccgcgttc ctcgtcgaca tcccgtcgga gggctggacc gaggtccgcc
      181 agatcgagac catgcacggc tcgacgggac actccgagat cctcatcgag gacctgcgcg
      241 tgcacgagtc gcagatgctg ggcgggcgcg gtcagggcca cctgctcggc cagtaccggc
      301 tgggcccggc ccgcctcgcg cactgcatgc gctggatcgc ccaggccgag accgcgctcg
      361 acatgatggt cgaccggtcc ctcaaccgct tcgcccacgg gtcgctgctg gccgagaagc
      421 aggggatcca gtggatgatc gctgactcca ccatggagct gtaccaggcc aagctgatgg
      481 tgctgcacgc cgcgtaccgg atcgacaacg ggctcgagtt caagtccgag gtgtcgatgg
      541 cgaagcactt cgtggccaac atgctgaacc gggtgatcga ccgctcgatc caggtccacg
      601 gcgccctcgg ctactcgacc gacaccccgc tcgcgcacat gtaccagcac gcccgctggg
      661 cccggttcgc cgacggggcc gacgaggtgc accagatgcg catcgcccag cggacgatcg
      721 cggcgtacaa ggactccggc tccaccgcct cggccaccgg cgacctcccg atctgaccca
      781 cgatcccgtt cccccctccc gttctgtagt gcgagatcca ggtgttcacc tggatctcgc
      841 actacagaac gggtttcggg gtgacgggcg cgaccagtgc cgtgagcggc aggctgggat
      901 ccgacagaga cagccgtccc cggcggggac ccgtgcgggg accgcgcagg ggaggcgcac
      961 atgtccacac ccaccgtccg gacccaccag ttgcgaacag ggcgtgcagg tcgcggcgcg
     1021 acgaccgacc tcggcgcctt cgtcgcggcc ctgatcggcg acgacccccc ggtcaggatc
     1081 gagctgttcg acggctcggc gctcgggccg ccccgctccg acgtgcccgc aaccgtgcag
     1141 gtccgctccc gcgacgccct ccggcggatg gtcgcctcgc ccggagagct cggcctggcc
     1201 cgcgcctacg tcgccggtga catcgacatc gacggcgaca tctacgcagt gctcggactc
     1261 cgcgaccgcc tgccccgcgt ccggctcacc ccccggttgt ggatcgacgc ggtccgcctc
     1321 ctgcgaccgt cggatctcgt accgctcccg ccgccgcccg aggaggcccg tccgggggcg
     1381 gtggcagagc tgaggtcccg gctcggccga ggacacacgc gggagaccga cgccgcgtcg
     1441 atccaccacc actacgacgt gtccaacgac ttctacgagc tcgtgctcgg tccggccatg
     1501 gtctactcgt gtgccctgtg ggacgaggac cgcaagcggc ggggcgccga ggccctggag
     1561 ccggcccagg acgccaagat cgagctgatc tgccagaagc tcgcgctccg acccggtctg
     1621 cgcttgctcg acatcggatg cggctggggg tccctcgtgc ggcacgccgc ccggcaccac
     1681 ggcgtccacg cggtcgggat caccatctcc gccgagcagg ccgcctgggc tcgggcgcgg
     1741 gtggaggccg acgggttggc ggaccgggtc gagatccggg tgcaggacta ccgcgacatc
     1801 cacgacgggc ccttcgacgc ggtgagctcg gtcgggatgt tcgaacacgt cgggctggcc
     1861 cggctcgctg agtacttcga gcaggtccac gccctgacgg cgccccgagg acggttcgtc
     1921 aaccacgcca tcagccgccc gcccgggcac gccccggcga tcgcccgccg cagcttcgtg
     1981 cagcgctacg tgttccccga cggcgagctg cacgaggtcg gctcggtcat caccgccatg
     2041 caagccgccg gcttcgaggc ccgccacatg gaggacctcc gcgagcacta cgcgctcacg
     2101 ctgcgggcct gggtggcgaa cctcgagcag aactgggatc gggccgtcgc cgatgtgggc
     2161 gtcaaccggg cccgggtgtg gcggctgtac atggccggat ccgcagtggg cttcgagcag
     2221 ggccggatcg ggatcaacca ggtgctcggg ttgcgcgacc cggaccaggc gcacttcccc
     2281 ctccgccccg actggacggc cgaccccgcg ccgccggccc gttctgtagt gcgagatcca
     2341 cacgaacgca tggatgtcgc actacagaac acggtggggc gggaccgctg agctccggag
     2401 ccggtcaatc ggtgggcttg cgggcgcggc tcggtgccac ccggggcggc tcgcccggca
     2461 tcttgggata gaccggcggc cacggagcgt ccatcaaccc gcgctcacgg tccgcctcgg
     2521 ccagggccag caggggctcc agcgactggg gggtcgtgcg catcggctcc cacggatccc
     2581 cccgctcggc gacgagaccg gggaccgtcg cgacggtcaa ctcatcaggg tggacatcgg
     2641 cgacttcgtc ccagctcagc ggggtcgaca ccggcgccat cgaacgggcc cgcaccgacc
     2701 acgcgccgaa caccgtcttg tgcggcgcgt tctggttgaa gtcgacgaag acccgctcgc
     2761 cgcgctcctc cttccaccac gcgtcggtga tgaggtcggg gcggcggcgg gccagctccc
     2821 gggcgaccgc cactgcggcg gctcgcacct cgatcgcccc ccatcgcggc tccagaccca
     2881 ggtacacgtg cagcccgcgg ttgccggtcg tcttggggta gccgaccagg ccgagctcgt
     2941 cgagcagatc cttcagcgcg aacgccgcgg atcggatgtg ctcgaagcgg atccccggtg
     3001 acggatcgag gtcgatgcgc agctcgtcga cgtgttcggg atcggccgcg agggacggcc
     3061 acacgtggaa ccccaggcac cccaggttca ccgcccacac cacgtgcgcc aggtccgcga
     3121 tcaccagcgc ctcggagcgg gtcccgttcg gggtggacac gatcgtggtc tccagccagt
     3181 cagggcgtga ggcgggcacc cgcttctgga agaagttgtt gccgtcgacc ccgtcgggga
     3241 agcgctgcat cagcaccggg cgcccgccca tcgcccgcat caccggctcg cccaccgtca
     3301 ggtagtagtc gaccaggccg gccttggtga caccgggatc ggggaagaag accttgtccg
     3361 ggctggtgac ccgcacggac cgtccggcca cctcgacctc gacggcttcg ctgcgggcca
     3421 tggcggcaac ctacccttct ccccaacgcg gaacgggctg gggagaagcg gtgggagagg
     3481 agctggtgat gaggtcaggt cgagggtccc ggagtgccag gggtgcccgg ggtgcggtag
     3541 tcggactggt cgcgggagtg gcggtcctcg ccaccgcctg cggagcggtg aagccgccgc
     3601 cggtgctgaa cgcgctgcgc gtcgagggcg ggccagcagc ggccgaccag ccgcgctgtg
     3661 ccgagacccc cgaggggacc gaacccgacc gggtggatcc ggcggaggcg ggtctggacc
     3721 cggcgctctt tcgctcggca ctggactacg cggtggccaa ggggtcgcag tcggtccggg
     3781 tctaccgcca cggctgcctg atcggcaccg gctccaacga cccgtcggtc gactgggtgc
     3841 cgctgccggg ttggtcggcg accaagggcg tcgtgtccat gctcgccggt cgcgcggtca
     3901 cccttgggcg cctcgacatc gacgcaccca ttggcggcta cctgaacgtc cgggaccctc
     3961 gcaaggccgc gttgaccgca cggcagctcc tcaaccagac gaccgggctg cgcatggcgt
     4021 gggtgagcga cctgaacgaa gcggccacca ccgactcggt cgccgcgctg atggatcgtc
     4081 ccttcgaggc cgttccgggc accaccttcg actacgccca gacgacggtg accgccctcg
     4141 tcgcagtcgt cgaggccgcc gtcggggagg acctccagtc cttcgcccag cgggagctgt
     4201 tcgggccgat cggcgtcggg agcgacgagt ggacctggga gcgcgacggg gcgggccgga
     4261 cccaggggtt cgccttcctg tcgatgacgc ccacggcgtt cggtcgcctg ggtcggctgc
     4321 tgctccagaa cggcgcgtgg gatggccgcc ggctgatctc cgccgactac atcgctcagg
     4381 gccgggcggg gacggcggcg aacccgtgct acgggttcct gtggcgcaac aacgacggcc
     4441 ggggctgcca ccagaccggt ccgctgctcg gcctggagac gaccggcaac tggatgccga
     4501 cggttcccga ggacgcctac ggcctgtcgg gcatgttcga ccaactggtc ctggtcatcc
     4561 cgagcctcga catggtggtg gtgcggctcg ggctgcccca ccaggtgatg gccgacccgt
     4621 ggggcgacat cggtggggag aagccggcga tgacgtggcg gttcttccgg acgctgatgg
     4681 cgtcggtcac cgacaccgtg gtggcggatc cgggggagtg gcagccctcg acgcccgggt
     4741 ccatcgactg ggcccacatc ttcgcggtcc cgctacccga gccgacctgg tgatctgagg
     4801 tccggatctg catcgcacgg tggggtccga tcccgggtgt gtcgcggtcc gatcaggaac
     4861 gggaccaggc acagcacggc gtaggaggcg acgacgcccc agaacaggcg gtcgccgggg
     4921 tgctccaggt atcgggccag gccgactccg accgccggcg cggccagcaa cgtgagcggg
     4981 atctgccggg tggccgagta gcggatcagg ccgaggtgct caccccacca ctcgatcacc
     5041 acgggtaccg gcagcagcgc gaccagccac gggtcgaggg cgtccggcca gcggaggccg
     5101 gccagagtga gcatcatcgt ggccaggcac gccggccaga accacaggca gcgtcggcag
     5161 acgtgacggt ggccgatccg cacgcagcgg tcgtagtcct cgggccagtg gtggctgagc
     5221 cacagcgcgg tgcgtcgctc cacggtctcc tatcggcggg agtcgtgcga tcccgcgttt
     5281 ctgaggtcgg gaaagcgaca aggaatgtcg ttgaggaccc ctatgtctgg tggagggggc
     5341 gggcggtggc ccgggcgatc tcggtggcca tgcccaggca ctcggggatc actcccatgc
     5401 cgaagaaccc gtggatcatg tcgtcgtagc agcggtacca caccggcacc ccggcgtcgg
     5461 acaggcgctc cgcataggcc gacccctcgt cgcgcagcgg gtcgaacccg gcggtggcca
     5521 ccacggccgg cgccaccccc tccaccgacg gcgccagcaa cggcgacacc gtgggcaggt
     5581 cccaggtgga gcgatccggc aggtactggg cccggtacca ctccatgtcg acgcgggtga
     5641 gtccgaaccc ctcggcgaac agctcgtgcg acggcttcga gaacgacgcc tcggtcgccg
     5701 ggtagacgag cgcctgcacc tgcggcgggc cgaccgggtc gtcgagggtg acctgggcga
     5761 ggaccgcggc gaggttgccg cctgcgctgt cgcccatcac cccgacccgc cgcccgtcga
     5821 tgccgagctc gcgggcgtgg gttcgcaccc agtggtacgc ggccaggctg tcgtcgagcg
     5881 gggcggggaa cgggtgctcg ggggccagtc ggtagtcgac cgagacgacc acgcagcggg
     5941 cctctgcggc gaccagtcgg caggtcccgt cgtaggcgtc gagcgagccc accacccatc
     6001 cgccgccgtg gatgtagagc agggccggcg ggtcgggttc gagtccgaac ggccggtaca
     6061 cacgaaccgc caacgtcgac tccggtccgg ggatccgacg ctcggagacg tgcagtccct
     6121 gtcgtgacgg catgccggtg gtggccatcc ggtccatctc ccgtcggcgc tggtcggggt
     6181 cgaacgagcg ggagccgtct ccgggcatca gccgctcacc gatggcgagc agggcctgga
     6241 cccgtcggtc gagcacccgg ccgtcgagga cgatgggcgg cgcgccgatg atgcggtcga
     6301 gcaccggcgc cggcgcggcc aggagggacc gggcggcgcg gtgagccatc gtgacgcggt
     6361 agcggggctc gaaccggcgt gcggcggttc cgtcggcggt cggaggcatg aagtcgtcgg
     6421 agagcacgcc gcgatcctgc cattcatgtg aggtgcgcgg tttctgaggt cgggaaagcg
     6481 acaaggtgtg tcgttgagga cccctatgtc cggaggacgg tgccggggcg ggcgccggtg
     6541 ggttcgccgt cgcggcgcac cgccaccccg ttgaccagca cgtcggacac gccgaccggc
     6601 tggtcagcgg tcagccgctc gccatcggcg gggaagtcga ccacccgtcg tagcggtccc
     6661 ggcgcgaccg tcgccgggtc gaacaccacg acgtcggccg cccgaccccg ggccagcacc
     6721 ccgcggtcgg ccaggccgaa cacggctgcc ggctcggcgg tcagcttgtg caccgcccgc
     6781 tcgatcggca tcacgccacg gtcgcggacc cagttcccga gcaggtcggt gctgaagcac
     6841 gcgtcgcaga gctggctgac gtgcgcgccg gagtcggcca gccccagcag cacgccgtcg
     6901 cggggaagca gccactcgat ggcatcgggg tcgtcgttgg ccagcaccga ccagaaccgg
     6961 gtgcgcaggt cgtcctccag cgacaggtcg accagcacgt cgagcggcga gcagccccgc
     7021 tccaccgcga cgtcgagcac gccccggccc accagctcgg ggtgccggtc ggactccgca
     7081 accgcgagcg acgcgtagtt gaacgggatg aacccctcgc cgtcgaggtc gcgtcgggcc
     7141 cgctcgcgcc actgcggatc gcgataggcg gccaggcgct cgtcgcgact gcggtccatc
     7201 agctcccgga acgagtcgcg ggtgttgagg gtgaacggct cggccaggtt catctggaac
     7261 accagcggcc ggcacgacac ctgcggccac acgtcgacgc cgcgggcccg ggcctcgtcg
     7321 tgctcggcga tgacgccctc gtggtagggg aggcccttga tcgtcagcag cgcggtccag
     7381 gtgaacggtc ggccgatctc ctgttggagg tcgaagacct gctggttggt gaacacgccg
     7441 cccggcagca tcgccacgac gcctcgaccc gcgtcgcgca gcggggccat cagcgtccgc
     7501 agctcgtcca ggtcggcccg acggctgggc acgggccggc cccggtcgcc gttgtgggtc
     7561 ggcgacgcgc tggtggcgaa gccgatcgct cccgccgcga tcgcctcggc gacggcgtcg
     7621 cgcatccggg tgagctcgtc ggcggtgggg tctcgctcgt aggcgtcgtc gcccatcgcc
     7681 cacagccgca gcgcggtgtg cccgatgtag cagccgaagt tgagcgccgg gctccgccgc
     7741 tcgaccgcgt cgaggtactc accgaaggtc tcgaactcgt cccagggcac cccgacggtc
     7801 agggtgtcca ggctcatgtc ctcgacgtgc tgcagcgtgc gggccagcag cccgacgccg
     7861 tcagcaccga tcggagcgat cgagaacccg cagttgccgg cgacgacggt ggtcacgccg
     7921 tggtgcagcg acggggtcag gtccgggtcc cagaacacct gtgcgtcgta gtgggtgtgg
     7981 acatcgacga agccgggggt caccacccga tcggtggcgt cgatcgtctc cactccggtc
     8041 ggtgccgaca cgtcggtgcc gacctcggcg atcaccccgt cgcggacgag cacgtcggcg
     8101 gggaagcccg gcgatcccgt tccgtcgatg accgttccac ccctgatgac gacgtcgacc
     8161 atgggtcggg ttctacacga ccgtgccgtt cagtactgga tgagggccga ggtcgggatg
     8221 accggttcgg cgttgagcac cacgtcggag aacggctggt tccaccgctc gtacttgggt
     8281 cgggcggctt cgcgcatgcg ggtggtcgag tgcaccgggt ccttgtcgaa ctcggggatg
     8341 acctgcgtgc cgaacagctc gatcatctcc aggttctcct cgtgggtgac gccctcgggc
     8401 gggaacccga acaccagctg gtcgacgccg accgactggt agttgttcac ctgctcgagc
     8461 acctcttcgg gggtgccgca cagcatccag ccaccggcga tcagctggtc gagcacctcc
     8521 tcgtcgggga tggagaacgg cggctccggc cacaccggtg ccccctcgcg cttgggcatc
     8581 gtgtcgtggt agagcgcgac catcgagttc aggtaccccc gccctcgact cagagcgatc
     8641 tcgcgggccc gggcccggtc gttcaggcag atgaccgcgt tggtcatcat gatgttgttg
     8701 ttctggtact gcccgagcgg ctcggtgcag gc
//
LOCUS       DAOQOD010000131         8586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3037_length_8586_cov_8.479194, whole genome shotgun sequence.
ACCESSION   DAOQOD010000131 DAOQOD010000000
VERSION     DAOQOD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_8586_cov_8.479194"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1007
                     /locus_tag="PLZ72_11910"
     CDS             99..1007
                     /locus_tag="PLZ72_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37048.1"
                     /translation="MAGPALRRAREMAGLDRADAARRVGVGRRDLRGIESGVRPVSTT
                     VLERAISVYGDDDLDLPPRQDLRHPTHPHLLVIGDEVVHVDPFRDDDSDVLADYVAAV
                     RRQRGLGPDAEIRFRSADLVQLASVLDLAAPDLERRLRRAAKMDDVPARRAARLLVLT
                     GLALALAGATERSARDMAWRPPLSGVPLFDVTARSARTRPIDSRIPAAGLVPNGAVPV
                     PELFTTREQTGAVVRDSSWLARDGRRPAFSTEPRVRLDAAAEVVANVVAAASGELAGP
                     RWAIEPAALPAAPAALAVAPTAAPTA"
     gene            1088..1903
                     /locus_tag="PLZ72_11915"
     CDS             1088..1903
                     /locus_tag="PLZ72_11915"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37049.1"
                     /translation="MNDEPAPLTPDAVRLDGRVVVVTGAANGIGRATAVAAAAFGASV
                     AVCDRDADGLAVLVDELARDRSVGPDRVHAEVLDVRDRDSVAAFVDATVARFGVVHGL
                     VNNAGGTYRAEFVDSAAKGDRALVDENFTSVVDVTRAVVEAMGAGGSIVNVTSSEAFQ
                     AAPGFSVYAAMKAAVENLTRTLALELADRGIRVNSVAPDGIPTHGDAALAEAVQAESR
                     FRPPPVPPIGRFADPSEAASVIVFLLGDLARFVTGTTLHVDGGLHAAGGWHRI"
     gene            2021..4564
                     /locus_tag="PLZ72_11920"
     CDS             2021..4564
                     /locus_tag="PLZ72_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37050.1"
                     /translation="MLRLALRSARAHWRRFVLTATAVVVGVAFVVAAFVLTDSLARSI
                     RDLLRDTAGRSDIVVRAATGSTGGPGGVFGGSRAGVPAALLPELAAVPGVAVADGIVS
                     GPGQLLDKGGRSGAFDFSLVSNWPVEPDLFGVRLASGRAPSGPDDVVIDAETAAERGL
                     GLGDRVRVATRRGIVDARISGIAEQRGTGLITAASVLAFSSERAAELVGVPGWYSVVN
                     IGLEPGARSDDVQSALRKVGGASVSVFSSEALVAAAQADIEEELTNFVGLLLGFAGVT
                     LFVSAFLIWNTFTIVVAQRTRELALLRAVGASRRQVAASVIGEGLVVGAAASALGLGL
                     GVLLGMGLQRLIVALGVALPLDSVVFEPRTAVAGVAVGLGVTAVSVVGPARRATAVPP
                     VAAIQSIDATPTRRSLGRPVVGSVLLGAGLVIGARALAMPTEPVDPRVKGVALGALLG
                     FLGVAALARFLAGPIVSVLGAPARATGVAGRIAARNSVRNPRRTASTASALMIGLALV
                     ATTLVVGESVRTAIRGGLTRSVHAEVVVDSGSVAPFDAATIDAIAAAPGVAEAVPMSI
                     TRSDTVGAPGRLAMTTGDVEQMASVVDPEIVEGRSPRGADEMAVNRRWADDKGVELGQ
                     RLTLKGGDVTRTLEVVGVYQRRELYDDTFAPTAAVAGLLGVEPAARLVFVRTEPGADP
                     ARVAAELARVAASIPNSTAQVRADFVESRTGSVDIIISIVNVLLGFAVAVAALGIANT
                     LALSVVERTRELGLLRAVGMSRRRMRLMVRLEGVLIGTFGGVLGLVVGVGFGAALVAA
                     LPAENAELTLPWARLAALAVLGSVLGIAAAALPARRAGRLDVLDAIAEE"
     gene            complement(4598..6163)
                     /locus_tag="PLZ72_11925"
     CDS             complement(4598..6163)
                     /locus_tag="PLZ72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HNA37051.1"
                     /translation="MTATGSPADGDHIVVTSTHGDLRGEWRDGVARFAGIPFAAAPVG
                     PLRFRPPESREPWDGVRDATAFGSVSPQNPSMMDALFGGEAEAWDEDCLHLNVWTTAP
                     GSPDGGADGSDALPVMVWIHGGGFEMGSGSSPLYDGTRFAHDGVVFVSINYRLGSLGF
                     LDLTSVDPAESGSGNVGLLDQVAALEWVRDNIAAFGGDPDNVTIFGESAGAMSVSLLL
                     TVARARGLFHKAISQSGALTAKSPGNAAVDTAEFMAAAGVDSVAGLRELSVEELLKAH
                     AAVSAARLADPDAIVRRTGSPVAFLAFTPVADGVVVPTDPEAAVAAGSAAGVPLLIGS
                     NLEEWKLFALMAPAPVDDDALRRRFELVTTDVDGALDAYRADDGDRSAADVECALLTD
                     VVFRIPAVRLADAQVAHAPVHQYRFDWRSPAFGGMIGAAHAVEIPFVFHMVGDHRLHV
                     LIGPDAPTELADAMHDSWVSFARSGVPRTATMNTWPSLPVTATERPLMLFDTEITLVE
                     DPQPATRRFWTGR"
     gene            6357..>6547
                     /locus_tag="PLZ72_11930"
     CDS             6357..>6547
                     /locus_tag="PLZ72_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNA37052.1"
                     /translation="MSVRNGLLALLASNPRHGYELKKELEERTGSLWELNVGQVYTTL
                     SRLERDGLVVEVDGAHPSSG"
     assembly_gap    6548..6647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6648..7455
                     /locus_tag="PLZ72_11935"
     CDS             <6648..7455
                     /locus_tag="PLZ72_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37053.1"
                     /translation="NDEPTREGETVTEVIEPTGDSTGPADDSTGSGAPDQEVATVLRL
                     VDIVQTVPDGRYRRVVLDGVDLDVGAGEMVVLMGPSGSGKTTLLRIAAGIDRPERGMP
                     VVDGEHLWVLSASQLAELRRRSIGYVEQRWNLLDSLTVVENVALPLELDGTGSREATR
                     DAMAALEDVGIAELARQFPSSLSGGEQQRAAVARALVGTRHVLVADEPTGALDALTAE
                     AVMRLIRGRCDRDGTAVLLATHDPAVAGWADRVVYLREGQLSDHVARAAR"
     gene            7452..>8586
                     /locus_tag="PLZ72_11940"
     CDS             7452..>8586
                     /locus_tag="PLZ72_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37054.1"
                     /translation="MSAGSISSTRALLRRSRRLAVRSPLRTLWTGLLVLVAVAAGAMV
                     MSADWGQVARSRSPERNLGAADAVYLVSDWPAEQTPALTDALTAALPDGSAVAVQQVV
                     SGLTLTATTSTGGGEDPSGRAAYGAAYQADWSAPELAGVLHLVEGRAPRDGEAVLTAD
                     TASTLGLGVGDHVRIDFSAVPLTVVGVGTVGPPDEAGVVLSEGQLVPAEGSAAVSAIR
                     VYVDLPAGTEPPPADDISAALAAGAGTVSPTSSIVGPVTPSMLSRASTAKPDAWMLAT
                     SDPGAPLARSAGLLVAVLVVVGVLAGAAFGLGATRRMRATGLLAANGADDGHLVTAAA
                     SEALVVALPAAVLGVVVAWAARGPWIRLRLPGWSSLVDATLPWL"
BASE COUNT         1020 a         2928 c         3327 g         1211 t
ORIGIN      
        1 ttcagtccag cccgcgacgt gccgatcttc atggcatgtc caacagggaa gggaaccgat
       61 cgggtgccgg tcaccggact gcggccgggg tcgatgtgat ggccggaccc gcgctgcgcc
      121 gcgccaggga gatggccggt ctggaccggg ccgacgccgc acgtcgtgtc ggcgtgggcc
      181 gtcgtgacct ccgggggatc gagtccggcg tccggccggt gagcacgacg gtgctcgagc
      241 gggcgatctc ggtgtacggc gacgacgacc tcgacctccc gccccgacag gacctccgcc
      301 accccaccca tccgcacctg ctggtgatcg gtgacgaggt ggtccacgtc gatccgttcc
      361 gggacgacga ctccgacgtg ctggccgact acgtggccgc ggtgcgacgg cagcggggtc
      421 tgggccccga cgccgagatc cgcttccgct ccgccgacct ggtgcagctg gcgtcggtgc
      481 tcgacctggc cgctccggac ctggaacgtc gactgcgacg agcggcgaaa atggacgatg
      541 ttccggcccg tcgggctgcc cggctgctcg tgctcaccgg cctggccctg gcgctcgccg
      601 gagcgaccga gaggtccgca cgggacatgg cgtggaggcc cccgttgagc ggggtgccgc
      661 tgttcgacgt caccgcccgt tccgcccgga cccgcccgat cgattcccgc atccccgccg
      721 cgggcctggt cccgaacgga gcggtcccgg taccggagct cttcacgacc cgggagcaga
      781 ccggtgctgt ggtcagggac tcgtcgtggc tggcgcgtga cgggcggcgc ccggcgttca
      841 gcaccgagcc ccgggtccgc ctcgacgccg ccgccgaggt ggtggcgaac gtggtcgcgg
      901 ccgcgtcggg cgagttggcc ggaccccggt gggcgatcga gccggcggcg ttgccggcag
      961 ccccggcagc cctggcggtc gcgccgactg cagctccgac ggcctgaaca gccgacctgc
     1021 atcggaccga cctgcatcag ggagtcggtc gggcccgccc gggggcgacg cggccatgat
     1081 gggcggcgtg aacgacgagc cggcaccgct tacacccgac gccgtgcggc tggacggccg
     1141 ggtcgtggtc gtcaccggtg cagcgaacgg gatcggtcgc gccaccgcgg tggctgcggc
     1201 cgcgttcggc gcgtcggtgg cggtgtgcga ccgtgatgcc gacggactgg ccgtcctggt
     1261 cgacgagctc gcacgcgacc ggtcggtcgg acctgaccgg gtccacgccg aggtcctcga
     1321 cgtccgagac cgcgactcgg tggccgcctt cgtggatgcg accgtggcgc ggttcggggt
     1381 cgtgcacggc ctggtcaaca acgcgggcgg cacgtaccgg gcggagttcg tcgactcggc
     1441 ggccaagggc gaccgggcgc tggtcgacga gaacttcacc agcgtcgtcg acgtgacccg
     1501 ggcggtggtc gaggcgatgg gcgcgggcgg ctcgatcgtc aacgtcacct cgagcgaggc
     1561 gttccaggcc gcgccgggct tctcggtgta cgcggcgatg aaggcggcgg tggagaacct
     1621 gacccgcacc ctcgccctgg agctcgcgga ccggggcatc cgggtcaact cggttgcgcc
     1681 cgacgggatc ccgacccacg gcgacgcggc gctggccgag gcggtgcagg ccgagtcgcg
     1741 gttccggccg ccgccggtgc cgccgatcgg gcggttcgcc gacccgtcag aggcggcatc
     1801 ggtgatcgtg ttcctgctcg gggacctggc ccggttcgtg accggcacga cgttgcacgt
     1861 cgacggcggg ctccacgccg ccggcggctg gcaccggata taggggtcct caacgacaga
     1921 ccttgtcgca atcctgacct cagaaagtcg ggggccggtc cgaaccggtc ggtgccgacc
     1981 cgacccccgg cagccgcccc cgggtgccag actcgccgcg atgttgcggt tggccctgcg
     2041 ctcggcccgg gcccactggc gtcggttcgt cctgaccgcg acagcggtgg tcgtcggggt
     2101 cgccttcgtc gtcgcggcgt tcgtgctgac cgacagcctg gcccgctcga tccgcgacct
     2161 gctgcgcgac accgcaggcc gttccgacat cgtcgtgcgg gccgcgaccg ggtcgaccgg
     2221 cggcccgggc ggggtcttcg gaggatcccg cgccggagtc ccggccgctc tgctgccgga
     2281 gctggccgcg gtgcccgggg tggcggtcgc cgacggcatc gtgtccggcc cgggccagct
     2341 gctcgacaag ggcggtcggt ccggggcgtt cgacttctcg ctggtcagca actggccggt
     2401 cgagcccgac ctgttcgggg tccggctggc gtcggggagg gcgccgagcg gtccggacga
     2461 cgtcgtgatc gacgccgaga ccgcggccga gcgcggactc gggttggggg accgggtccg
     2521 ggtcgcgacc cgacggggga tcgtcgacgc ccgcatctcg gggatcgccg agcagcgagg
     2581 caccgggctg atcaccgcgg cgtcggtgct ggccttctcc tcggagcggg ctgccgaact
     2641 ggtcggcgtc cccggctggt acagcgtggt caacatcggc ctggaacccg gcgcgaggtc
     2701 cgatgacgtg cagtcggcgc tgcggaaggt cggcggggcg tcggtgtcgg tgttcagctc
     2761 ggaggcgctg gtggccgcgg cccaggccga catcgaggag gagctcacca acttcgtcgg
     2821 gctcctgctc gggttcgcgg gtgtgacgtt gttcgtcagc gcgttcctga tctggaacac
     2881 gttcacgatc gtggtggccc agcgcacccg ggagctggct ctgctgcggg cggtcggggc
     2941 gtcgcgtcgg caggtggcag cctcggtgat cggcgaggga ctggtggtcg gggcggccgc
     3001 gtcggcgctc ggcctggggt tgggcgtcct gctcggcatg ggcctccagc gactgatcgt
     3061 ggcgctcggg gtggcgctgc cgctcgactc ggtggtgttc gagccccgca ccgcggtggc
     3121 gggtgtggcc gtgggtctgg gcgtgaccgc ggtgtcggtg gtcggcccgg cccgacgcgc
     3181 cacagcggtt ccgccggtgg cggcgatcca gtccatcgac gccaccccga cccgacggtc
     3241 cctcggccga ccggtcgtcg gttctgtgct gctcggggcc gggctggtca tcggcgcccg
     3301 ggccctggcc atgccgaccg aacccgtcga cccgcgggtc aagggggtgg cgctcggcgc
     3361 gttgctcggg ttcctcggcg tggcggccct cgcccggttc ctggccggtc cgatcgtctc
     3421 ggtgctcggg gcgccggcgc gggcgaccgg ggtcgccggg cggatcgcgg cccggaactc
     3481 cgtgcgcaac ccgcggcgga cggcgtccac ggcgtccgcg ctcatgatcg ggctggccct
     3541 ggtggcgacc accctggttg tcggcgagtc ggtgcggacc gcgatccgag gcgggctgac
     3601 ccggtcggtg cacgctgagg tggtggtcga ctccgggtcg gtggccccgt tcgacgctgc
     3661 gacgatcgac gccatcgccg cggcgccggg cgtggccgag gcggtgccga tgtcgatcac
     3721 gcgctccgac accgtcggcg cgcccggtcg cctcgccatg accaccggcg acgtggagca
     3781 gatggcgtcc gtggtcgacc cggagatcgt cgagggccgc tcgccgcggg gagccgacga
     3841 gatggcggtc aaccgccgct gggccgacga caagggggtg gagctcgggc agcggctcac
     3901 cctgaagggc ggcgacgtca cccggaccct cgaggtggtc ggcgtctacc agcgtcgtga
     3961 gctctatgac gacaccttcg ccccgaccgc ggcggtcgcc gggctgctcg gcgtggagcc
     4021 ggctgcccgc ctggtgttcg tgcgaaccga gccgggggcc gacccggcgc gggtggcggc
     4081 ggagctggcc cgggtcgcgg cgtccatccc gaactcgacg gcgcaggtgc gcgccgactt
     4141 cgtcgagagc cggaccgggt cggtcgacat catcatcagc atcgtcaacg tcctgctcgg
     4201 gttcgcggtc gcggtggccg ccctcgggat cgccaacacc ctggccctgt cggtggtgga
     4261 gcggacccgc gagctcggcc tgttgcgggc tgtcgggatg tcgcgtcgcc ggatgcggct
     4321 catggtccgg ctcgaggggg tgctgatcgg caccttcggc ggcgtgctgg gactggtggt
     4381 cggcgtgggc ttcggggcag cgctggtcgc ggcgctgccg gccgagaacg ccgagctcac
     4441 cctgccgtgg gcgcgcctgg ctgcgctcgc ggtgctcggg tcggtcctgg gcatcgcggc
     4501 cgccgctctg ccggcgcggc gggcgggtcg cctcgacgtc ctcgacgcga tcgccgagga
     4561 gtgagcgcga ccggggccgg tccgtcgggc ggcgtgctca gcggccggtc cagaaccggc
     4621 gggtggccgg ctgggggtcc tcgaccagcg tgatctcggt gtcgaacagc atcagcggcc
     4681 gctcggtcgc ggtgaccggc aggctgggcc aggtgttcat cgtggcggtg cggggcacgc
     4741 cgctgcgggc gaaggacacc cacgagtcgt gcatggcgtc ggccagctcg gtcggggcgt
     4801 cgggtccgat cagcacgtgc aggcggtggt cgccgaccat gtggaacacg aacgggatct
     4861 cgaccgcgtg ggcggcgccg atcatcccgc cgaaggcggg gctgcgccag tcgaagcggt
     4921 actggtgcac cggggcgtgg gcgacctggg cgtcggccag acgcaccgcg ggtatgcgga
     4981 acacgacgtc ggtgagcagc gcgcactcga cgtcggccgc ggagcggtcg ccgtcgtcgg
     5041 cgcggtaggc gtcgagcgcg ccgtcgacgt cggtggtcac cagctcgaac cgtcggcgca
     5101 gcgcgtcgtc gtcgacggga gcgggagcca tgagggcgaa cagcttccac tcctcgaggt
     5161 tggagccgat gagcagcggc accccggccg cggaaccggc agcgacggcg gcctcggggt
     5221 cggtgggcac gacgacgccg tcggcgaccg gggtgaacgc gaggaacgcg accggactgc
     5281 cggtccggcg cacgatcgcg tcggggtcgg cgagtcgcgc ggccgagacc gcggcgtggg
     5341 ccttgagcag ctcctcgacg ctcagctccc gcaggcccgc aacggagtcg acgcccgcag
     5401 cggccatgaa ctcggcggtg tcgaccgcgg cgttgcccgg agacttggcc gtcagcgccc
     5461 cggactggga gatggccttg tggaacagac cccgggcccg ggcgacggtc aacagcagcg
     5521 acacgctcat ggccccggcg gactcgccga agatggtcac gttgtcgggg tcgcctccga
     5581 aggcggcgat gttgtcccgc acccactcca gcgcggcgac ctggtcgagg agtcccacgt
     5641 tcccggaccc ggactcggcg gggtcgaccg aggtcaggtc gaggaagccg agggagccga
     5701 gccggtagtt gatgctgacg aacacgacgc cgtcgtgggc gaagcgggtt ccgtcgtaca
     5761 ggggcgacga acccgagccc atctcgaagc cgccgccgtg gatccacacc atgaccggca
     5821 gggcatcgct gccatcggcc ccgccgtccg gggagccggg agcggtcgtc cagacgttga
     5881 ggtgcaggca gtcctcgtcc cacgcctcgg cctcgccgcc gaagagggcg tccatcatcg
     5941 acgggttctg aggtgacaca gacccgaagg cggttgcgtc ccggacaccg tcccatggct
     6001 cgcgggattc ggggggacgg aaacggagcg ggccgaccgg cgcggcggcg aaggggatcc
     6061 cggcgaagcg ggcgacgccg tcgcgccact cgccgcgcag gtcgccatgg gtggatgtga
     6121 cgacgatgtg gtcgccgtcg gcaggggatc cggtcgcggt catggcgatg atctagcacc
     6181 cggacccttc tccatgtccg cagaactggt cggcggggcg atcgcggcct cgtcgcaatc
     6241 ccgggttgcg ggacccgcct actcggtata tacatacgga gtagctatac ggagtaggta
     6301 ccgaatccgg ccgatcgatc gagtcggccc gaacgacgag caggaaggga gagccgatgt
     6361 cggtgcggaa cgggctcctg gcactgctgg cgtcgaaccc ccggcacggc tacgagctca
     6421 agaaggagct cgaggagcgg accggctcgc tgtgggagct caacgtcggg caggtgtaca
     6481 cgaccctctc ccgtctggag cgcgacgggc tggtggtcga ggtcgacggt gcccacccat
     6541 cgtcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa cgacgaaccg
     6661 acgagggaag gggagacggt gaccgaggtg atcgaaccca ccggcgacag caccggcccg
     6721 gccgacgaca gcaccggctc aggagccccg gaccaggagg tcgcgaccgt gttgcgactg
     6781 gtcgacatcg tgcagaccgt tcctgacggg cgctaccgtc gggtggtcct cgacggagtc
     6841 gatctggacg tcggcgccgg agagatggtc gtgctcatgg gtccatcggg ctcgggcaag
     6901 acgacgctgc tgcgcatcgc tgcgggcatc gaccggcccg agcggggcat gccggtcgtc
     6961 gacggcgagc acctgtgggt gctgtcggcg tcgcagctgg ccgaactgcg acggcgcagc
     7021 atcggctacg tcgaacagcg ctggaacctt ctcgactcgc tcaccgtggt cgagaacgtg
     7081 gccctgcccc tcgagctcga cggcaccgga tcccgcgagg ccacccgcga cgccatggct
     7141 gcgctcgagg acgtcggcat cgccgagctc gcccggcagt tcccgtcgtc gctgtcgggt
     7201 ggtgagcagc agcgcgcggc ggtcgcccgg gcgctggtcg ggacccgtca cgtcctggtc
     7261 gcggacgagc cgaccggagc gctcgacgcc ctcaccgccg aggcggtgat gcgcctgatc
     7321 cgcgggcgct gcgaccgcga cggcaccgcc gtgctgctcg ccacccacga cccggccgtg
     7381 gccgggtggg ccgaccgggt cgtctacctg cgggaaggtc agctctcgga ccacgtcgcg
     7441 cgggcggccc ggtgagcgcc gggtcgatca gctcgacgcg ggcgctgctg cgtcgctctc
     7501 ggcgtctggc ggtgcgcagc ccgctgcgca cgctgtggac cgggcttctg gtgctcgtcg
     7561 cggtcgccgc gggcgccatg gtgatgtcgg cggactgggg ccaggtcgcc cggtcgcggt
     7621 ccccggagcg caacctgggc gcggccgacg cggtctacct ggtgtcggac tggccggcgg
     7681 agcagacccc ggcgctgacc gacgcgctca ccgccgcact tcccgacggc tccgcggttg
     7741 ccgtccagca ggtcgtctcc ggcctcaccc tgaccgcaac gacttcgacc ggtggcggcg
     7801 aggacccgtc cgggcgtgcg gcctacggag cggcgtacca ggccgactgg tcggctccgg
     7861 agctggccgg ggtgctgcac ctggtcgagg ggcgcgcccc ccgcgacggt gaggcggtgc
     7921 tcaccgcgga cacggcgagc accctcggac tgggcgtggg cgatcacgtg cggatcgact
     7981 tctccgcggt tccgctgacg gtggtggggg tcggcacggt cggcccgccc gacgaggccg
     8041 gcgtggtgct gtcggagggc caactcgtcc cggcggaggg gtcggcggcg gtcagcgcga
     8101 ttcgcgtgta cgtggacctt ccggcgggga ccgagccgcc gcccgccgac gacatctcgg
     8161 cggccctcgc cgccggcgcg gggacggtgt cgccgacctc gtcgatcgtc ggtccggtca
     8221 ccccgtcgat gctgtcgcgt gcatcgacgg cgaagccgga cgcgtggatg ctcgcgacct
     8281 ccgacccggg tgcgccgctg gctcgctcgg cggggttgct ggtcgcggtg ctggtcgtgg
     8341 tcggggtcct ggccggcgcc gcgttcggtc tgggcgccac ccgccgcatg cgcgccaccg
     8401 ggctcctggc cgccaacggc gccgacgacg gacacctggt gacggccgcc gcgtccgagg
     8461 cgctggtggt ggcgctgccg gccgcggtcc tcggcgtggt cgtcgcctgg gcggcgcgcg
     8521 ggccgtggat ccggctgcgg ttgccgggct ggtcgtcgtt ggtcgacgcg acgctcccgt
     8581 ggctgt
//
LOCUS       DAOQOD010000132         8365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3131_length_8365_cov_12.579302, whole genome shotgun sequence.
ACCESSION   DAOQOD010000132 DAOQOD010000000
VERSION     DAOQOD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_8365_cov_12.579302"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2531)
                     /gene="rpoC"
                     /locus_tag="PLZ72_11945"
     CDS             complement(<1..2531)
                     /gene="rpoC"
                     /locus_tag="PLZ72_11945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNA37055.1"
                     /translation="MLDVNLFDQLRIGLATADAIRMWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECYCGKYKRVRFRGIICERCGVEVTRSKVRRERMGHIELAAPAV
                     HIWYLRGTRSWLAYLLSGIEPKEELKAKQLEKVIYFAANLVTHVDEDKRHADLESLEA
                     EMLGERDAIEKDRELELARRMEELEAELEALEAEGAKESDLTSRRKAADKDLTAIRET
                     YEDELELLNRVWDEFRDLYPRQIIEDETLWRELQDRYEDYFEGGMGASAIKLLIDRID
                     LVEEEEKLRAAIDPEEGRKPLSAQRKQKAIKRLKIVAAFNRTDDNGNRVNDPRAMILD
                     VVPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRM
                     LQEAVDALFDNGRRGRPVTGPGSRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGPTLKLHQCGLPKLMALELFKPFVMKKLVDAELAQNIKSAKRMVERRRPQVWDVLED
                     VIKEHPVLLNRAPTLHRLGIQAFEPILVEGKAIQIHPLVCAAFNADFDGDQMAVHLPL
                     SAEAQAEARVLMLSANNMLSPAHGKPLVTPTQDMIIGAFYLTSQTDGAAGEGRVYKRM
                     DQVERDLEAGNLSVHATIEYRGSEFPDLLESPANGSAAVWRKTTAGRLLFNRTLPEGY
                     EFVDHPVRKKEMTAIVDRLAIEFERAVAANSLDAMKALCFNYAMRSGLTISIDDVQTP
                     ADKYEILDRHEAEADKVEKQFRRGIITDGERKQKEVEIWTTATSEVTRAMQKAMQAKQ
                     FNPIDMMVGSGARGNMMQVRQIAGMRGLVANPRGDMIPRPIKSNFR"
     gene            complement(2551..6153)
                     /locus_tag="PLZ72_11950"
     CDS             complement(2551..6153)
                     /locus_tag="PLZ72_11950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNA37056.1"
                     /translation="MSSRVNFRDRYSFANLDEVLDLPDLIAIQRESFRWFVEQGLADA
                     FRDISPIKDFTETLSLELEFDPFDEDLRPPPKFTVEECKEKDMTFSAPIFARARFMNA
                     NTGEIKEQTVFMGDFPMMTDKGTFIINGTERVVVSQLVRSPGVIFQPGERFRLRNLSK
                     HQLVTGTIHPYRGEWIEFDVEQKPGKDPTAGTRVARKRRLSLFVLLRALGYDDANAPG
                     FLEAFVRHFDFLEGQWEKDKEIAPTQEEAIIEIYKRARPGEPATPDSARNYFNNAFFE
                     PRRYDLSRVGRYKLNRKLGPEIERLQEQFPQLAGLLDVPDPDQPVLSRCEVLAATTYL
                     LNLAAGTHGYRLDDQDHFANRRIRSVGELIQNQVRIGLSRMERVVRERMTTQDVEAIT
                     PTTLINIRPVVAAIKEFFGTSQLSQFMDQVNPLSGLNHRRRLSALGPGGLSRERAGFE
                     VRDVHFSHYGRMCPIETPEGPNIGLQGALATFARVNQFGFIETPYRKVENGRVTDEIV
                     YLPADEEEEYIVAQANTPIGPDGRFTADRVLVRRSPQAASLSDLKLQLERDEFLGATT
                     EISAVAPEEVQLMDVSPRQIVSVGTALIPFLEHDDANRALMGANMQKQAVPLLRAEAP
                     YIGTGMETRAARDAADMILAIEDGVVLEVDGGQIVVEYKKAGRVTHRLHKFERSNQDT
                     CINQRPAVAEGQKLAAGDVIAEGPSTDNGELALGKNLLVAFMPWEGYNFEDAIILSER
                     LVKDDVLTSIHIHEHEVDARDTKLGPEEITRDIPNLSEEILMDLDERGIIRVGAEVDP
                     GDVLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGKVIDVKVFSRD
                     DEHELPPGVNQLVRVYVAQKRKISVGDKLAGRHGNKGVISRILPIEDMPFLADGTPVD
                     IILNPLGVPSRMNVGQVLETHLGWAARWGWDDGDEHIGETPLRGTETKTRPTTRPATL
                     IATPVFDGAKWDEEDMAGRHPTIKTIFSNIEPESVRSDYGNDGRLIGSDGKATLYNGR
                     TGEAYDNPVTCGYAYILKLAHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEME
                     VWALEAYGAAYCLQELLTIKSDDTLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQA
                     LCLNVEVLSRTGEEIKMRELDEDFFRAAEELGIDLSRPERGSDEDDARRQAERR"
     gene            complement(6594..6980)
                     /gene="rplL"
                     /locus_tag="PLZ72_11955"
     CDS             complement(6594..6980)
                     /gene="rplL"
                     /locus_tag="PLZ72_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNA37057.1"
                     /translation="MATKEEILDAISNMTVLELSELLSEFEERFGVTAAAPVAVAAAA
                     PAAGGGDAGGGDDEKTSFDVVLTAAGDKKIGVIKEVRGLTSLGLKEAKDLVESAPKAV
                     LEGVSKEEAEKAKEAIEAAGGSVEIK"
     gene            complement(6986..7750)
                     /gene="rplJ"
                     /locus_tag="PLZ72_11960"
     CDS             complement(6986..7750)
                     /gene="rplJ"
                     /locus_tag="PLZ72_11960"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNA37058.1"
                     /translation="MENPRPEKVAVVEEVKDKFQTADAVIITEYRGLTVRDLQDLRTS
                     LREAGGEYHVYKNTLVRRATASLDLELDEHLLGPTALTFVGSKADGTPGDVVTVAKAL
                     KSFAKSNPLLVMKGGVLGDSLLDQAALSRLAEVAPREELLARLAGGMAAPMQNFASLL
                     NAIPQKMAYALQALIEAGGASDAPASVADEPTEPAADEAATQEPTAEADPADTAEAEA
                     PAQADASTDTEDQDQTPAEGGDAAEADADDAAQEQE"
     gene            complement(8133..>8365)
                     /locus_tag="PLZ72_11965"
     CDS             complement(8133..>8365)
                     /locus_tag="PLZ72_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNA37059.1"
                     /translation="FKGGKVEYRTDRNGNVHVAIGKASFDAAALVANFGAIIDELIKA
                     KPSAAKGRYIRKVTLSSTMGPGVQLDVHRLEG"
BASE COUNT         1126 a         2720 c         3012 g         1507 t
ORIGIN      
        1 cggaagttgg acttgatcgg acgggggatc atgtcgccgc gggggttggc caccaggccg
       61 cgcatgccgg cgatctggcg gacctgcatc atgttccctc gggcgcccga gccgaccatc
      121 atgtcgatcg ggttgaactg cttggcctgc atggccttct gcatggcccg ggtcacctca
      181 gaggtcgcgg tcgtccagat ctcgacctcc ttctgcttgc gctcgccgtc ggtgatgatg
      241 ccgcgccgga actgcttctc gaccttgtcg gcctcggcct cgtggcggtc gaggatctcg
      301 tacttgtcgg ccggcgtctg cacgtcgtcg atcgagatcg tcagcccgga gcgcatggcg
      361 tagttgaagc agagcgcctt catggcgtcg agggagttgg cggccacggc gcgctcgaac
      421 tcgatggcca gacgatcgac gatcgcggtc atctccttct tgcggacggg gtggtcgacg
      481 aactcgtagc cctcgggcag cgtccggttg aacaggaggc gaccggcggt ggtcttgcgc
      541 cacaccgcgg cggacccgtt ggccggcgac tcgagcaggt cggggaactc gctgccccgg
      601 tactcgatgg tggcgtgcac gctgaggttg ccggcctcga ggtcgcgctc gacctggtcc
      661 atgcgcttgt agacgcggcc ctcgccggcg gcgccgtcgg tctgcgacgt caggtagaag
      721 gccccgatga tcatgtcctg ggtgggggtg accagcggct tgccgtgcgc ggggctcagc
      781 atgttgttgg cgctgagcat gaggacgcgg gcctcggcct gagcctcggc cgacagcggc
      841 aggtgcaccg ccatctggtc gccgtcgaag tcggcgttga acgccgcgca caccagcggg
      901 tggatctgga tggccttgcc ctcgaccagg atcggttcga aggcctggat gcccaggcgg
      961 tgcagcgtgg gcgcacggtt caggagcacc gggtgctcct tgatgacgtc ctcgagcacg
     1021 tcccacacct gcgggcgacg acgctcgacc atgcgcttgg ccgacttgat gttctgggcc
     1081 agctccgcgt cgaccagctt cttcatgacg aagggcttga acagctccag ggccatcagc
     1141 ttgggcagac cgcactggtg cagcttgagg gtggggccga ccacgatgac cgaacggccc
     1201 gagtagtcga cgcgcttgcc gagcaggttc tggcggaacc ggccctgctt gcccttcaac
     1261 atgtccgaca gcgacttgag cgggcggctg cccggaccgg tgaccggtcg accgcggcgg
     1321 ccgttgtcga acagcgcgtc aacggcctcc tgcagcatgc gcttctcgtt gttgacgatg
     1381 atctccggcg cgcccaggtc cagcagccgc ttgaggcggt tgttgcggtt gatgacgcgg
     1441 cggtacaggt cgttgaggtc ggaggtggcg aagcggccgc cgtcgagctg caccatcggg
     1501 cgcaggtccg gcgggatcac cgggacgacg tcgaggatca tggcgcgggg gtcgttgacc
     1561 cggttgccgt tgtcgtcggt gcggttgaac gcggcgacga tcttcaggcg cttgatcgcc
     1621 ttctgcttgc gctgcgcgga gaggggcttg cggccctcct cggggtcgat ggcggcgcgg
     1681 agcttctcct cttcctcgac caagtcgatg cggtcgatga gcagcttgat ggcgctcgcg
     1741 cccatgccgc cctcgaagta gtcctcgtag cggtcctgca gctcgcgcca gagggtctcg
     1801 tcctcgatga tctgacgcgg gtacaggtcg cggaactcgt cccagacccg gttgagcagc
     1861 tcgagctcgt cctcgtaggt ctcgcggatg gcggtgaggt ccttgtcggc cgccttgcgc
     1921 cggctggtga ggtcggactc cttggcgccc tcggcctcga gggcctcgag ctcggcctcg
     1981 agctcctcca tgcgccgggc cagctcgagc tcgcggtcct tctcgatcgc gtcgcgctcg
     2041 ccgagcatct cggcttccag gctctccagg tcggcgtggc gcttgtcctc gtcgacgtgg
     2101 gtcaccaggt tggcggcgaa gtagatgacc ttctcgagct gcttggcctt gagctcctcc
     2161 ttgggctcga tgcccgacag caggtaggcc agccacgagc gggtgccgcg caggtaccag
     2221 atgtgcaccg ccggcgcggc gagctcgatg tggcccatcc gctcccggcg caccttggag
     2281 cgggtgacct cgacgccaca gcgctcgcag atgatgccgc ggaaccggac gcgcttgtac
     2341 ttgccgcagt agcactccca gtccttggtc ggaccgaaga tcttctcgca gaagagcccg
     2401 tccttctccg gcttgagcgt gcggtagttg atggtctccg gcttcttgac ctcgccgttg
     2461 gaccacatgc ggatggcgtc cgcggtggcc aggccgatcc ggagctggtc gaagaggttg
     2521 acgtcgagca tgggggtggt cgttcctctc tcagcgacgc tccgcctggc ggcgggcgtc
     2581 gtcctcgtct gatccgcgct cggggcggga caggtcgatg cccagctcct cggcggcgcg
     2641 gaagaagtct tcgtcgagct cccgcatctt gatctcctca cccgtgcggg agaggacctc
     2701 gacgttcaaa cacagggcct gcatctcctt gatgagcacc ttgaaggact cggggatgcc
     2761 gggctcgggg atgttctcgc ccttgacgat cgcctcgtag accttgacgc ggccgagggt
     2821 gtcgtcggac ttgatggtca gcagctcctg caggcagtac gccgctccgt aggcctcgag
     2881 cgcccacacc tccatctcac cgaagcgctg gccaccgaac tgggccttac cgccgagcgg
     2941 ctgctgggtg atcatcgagt acgggccggt cgagcgggcg tggatcttgt cgtcgaccag
     3001 gtgggcgagc ttcaggatgt acgcgtagcc gcaggtgacc gggttgtcgt aggcctcgcc
     3061 ggttcggccg ttgtagaggg tggccttgcc gtcggagccg atcaggcgac cgtcgttgcc
     3121 gtagtcggag cggaccgact cgggctcgat gttggagaag atggtcttga tggtcgggtg
     3181 acgaccggcc atgtcctcct cgtcccactt ggcgccgtcg aacaccgggg tggcgatcag
     3241 ggtggcgggg cgggtggtcg ggcgggtctt ggtctcggtg ccgcgcagcg gggtctcgcc
     3301 gatgtgctcg tcgccgtcgt cccagcccca gcgggcggcc cagcccaggt gcgtctcgag
     3361 cacctggccc acgttcatgc gggacggcac gccgagcggg ttcaagatga tgtcgaccgg
     3421 ggtgccgtcg gccaggaacg gcatgtcctc gatgggcagg atccgggaga tcacgccctt
     3481 gttgccgtgg cggccggcga gcttgtcgcc gacggagatc ttgcgcttct gggccacgta
     3541 gacccgcacc agctggttga cgccgggcgg cagctcgtgc tcgtcgtcgc ggctgaacac
     3601 cttgacgtcg atgaccttgc cggtctcgcc gtggggcacc ttgagcgagg tgtcgcggac
     3661 ctcacgggcc ttctcgccga agatggcgcg cagcaggcgc tcctcggggg tgagctcggt
     3721 ctcgcccttg ggggtgacct tgccgacgag cacgtcgccc gggtcgacct cggcgccgac
     3781 gcggatgatg ccgcgctcgt cgaggtccat caggatctcc tcggagagat tcgggatgtc
     3841 ccgggtgatc tcctcggggc ccagcttggt gtcgcgggcg tcgacctcgt gctcgtggat
     3901 gtggatcgag gtcaggacgt cgtccttgac caggcgctcc gagaggatga tggcgtcctc
     3961 gaagttgtag ccctcccacg gcatgaaggc gacgagcagg ttcttgccca acgccagctc
     4021 gccgttgtcg gtggaggggc cctcggcgat gacgtcgccg gcggccagct tctggccctc
     4081 ggcgaccgcg gggcgctggt tgatgcaggt gtcctggttg gagcgctcga acttgtggag
     4141 gcggtgggtg acccgaccgg ccttcttgta ctcgacgacg atctggccgc cgtcgacctc
     4201 gagcaccacg ccgtcctcga tggccaggat catgtcggcc gcgtcgcgcg ccgcgcgggt
     4261 ctccatgccg gtgccgatgt agggggcctc ggcccgcagc agcggcacgg cctgcttctg
     4321 catgttggcg cccatgagcg cgcggttggc gtcgtcgtgc tcgaggaacg ggatgagcgc
     4381 ggtgccgacc gagacgatct ggcggggcga gacgtccatg agctggacct cttcgggtgc
     4441 caccgccgag atctcggtgg tggcgcccag gaactcgtca cgctcgagct gcagcttcag
     4501 gtccgacagc gacgccgcct gcggcgaacg acgcaccagc acgcggtcgg cggtgaagcg
     4561 gccgtcgggg ccgatcggcg tgttggcctg ggcgacgatg tactcctctt cctcgtccgc
     4621 gggtaggtag acgatctcgt cggtgacccg gccgttctcg accttgcggt acggggtctc
     4681 gatgaagccg aactggttga cgcgggcgaa ggtggccagc gcgccctgca gaccgatgtt
     4741 ggggccttcg ggagtctcga tcgggcacat ccggccgtag tggctgaagt gcacgtcgcg
     4801 gacctcgaag ccggcgcgct cgcgggacag gccaccgggg ccgagcgccg agaggcgacg
     4861 gcggtggttc aggcccgaca gggggttgac ctggtccatg aactggctga gctgggaggt
     4921 cccgaagaac tccttgatgg cggccacgac cggtcggatg ttgatcaggg tggtcggggt
     4981 gatcgcctcg acgtcctggg tggtcatgcg ctcacgcacg acgcgctcca tgcgcgacag
     5041 gccgatgcgg acctggttct ggatcagctc gccgaccgag cggatgcgcc ggttggcgaa
     5101 gtggtcctgg tcgtcgaggc ggtagccgtg ggtgccggcg gccaggttga gcaggtaggt
     5161 ggtggcggcc aggacctcgc agcggctgag caccggctgg tcggggtccg gcacgtcgag
     5221 caggccggcg agctgcggga actgctcctg gagacgttcg atctcggggc cgagcttgcg
     5281 gttgagcttg tagcgaccga cccggctgag gtcgtagcga cggggctcga agaaggcgtt
     5341 gttgaagtag ttgcgcgccg agtccggcgt ggcgggctca cccgggcggg cccgcttgta
     5401 gatctcgatg atcgcctctt cctgggtggg ggcgatctcc ttgtccttct cccactgccc
     5461 ttccaggaag tcgaagtggc ggacgaaggc ctcgaggaag ccgggggcgt tggcgtcgtc
     5521 gtagccgagc gcccgcagca gcacgaacag cgacaggcga cgcttgcggg cgacgcgggt
     5581 gcccgccgtc gggtccttgc ccggcttctg ctcgacgtcg aactcgatcc actcgccgcg
     5641 gtaggggtgg atggtgccgg tgaccagctg gtgcttggag aggttccgca ggcggaaccg
     5701 ctcgccgggc tggaagatga cgccgggcga gcgcacgagc tggctgacca ccacgcgctc
     5761 ggtgccgttg atgatgaagg tgcccttgtc ggtcatcatc gggaagtccc ccatgaagac
     5821 cgtctgctcc ttgatctcgc cggtgttggc gttcatgaag cgggcccggg cgaagatcgg
     5881 cgcggagaag gtcatgtcct tctccttgca ctcctccacc gtgaacttgg gcggaggacg
     5941 caggtcctcg tcgaacggat cgaactccag ctcgaggctg agcgtctcgg tgaagtcctt
     6001 gatcggcgag atgtcgcgga aggcgtccgc cagaccctgc tcgacgaacc agcggaacga
     6061 ctcccgctgg atggcgatca gatcgggcag gtcgaggacc tcgtcgaggt tcgcgaacga
     6121 atagcggtcg cggaagttga cgcgagagga caaggagcta ctccctgttg ctgggcgagc
     6181 ggtgggcgct gagcgggcgg cgggtcatca ccgttccccg ggcgcgcgac tcctcagcgg
     6241 cagttccgtg tgaggggagg actgctgggg aggacgaacg gcagggcacg gcaaccgaac
     6301 aggttacgcg ggcgtgcggg gtcggtcaag cggcccgaag caggccgagc cggccggcag
     6361 ggcaccctgc tgagagggcg ccgcggcgga ccgtggcgat cggttccggg gagcgtacga
     6421 acatgacccg cgcaggtcaa gcacctccgc ctccgcctcc cccaccccgt ccccctcccg
     6481 ttctgttcgt cgtttcacaa ctccagtgat gtgaaacgac gaacagaacg ggcgagggcg
     6541 cccggccgga gccggacgcc ctcgcgtcga cggtcacccc cgaaggggcc cggtcacttg
     6601 atctcgacgg acccgccggc ggcctcgatg gcctccttgg ccttctcggc ctcctccttc
     6661 gacacgccct cgaggacggc cttgggggcg gactcgacca ggtccttggc ttccttgagc
     6721 ccgaggctcg tgagcccacg gacctccttg atgacgccga tcttcttgtc gcccgccgcg
     6781 gtgagcacca cgtcgaagct ggtcttctcg tcgtcgccac cgccggcgtc gccgccgccc
     6841 gcagccggag cggccgccgc cacggccacc ggggccgccg cggtcacgcc gaagcgctcc
     6901 tcgaactcgc tcagcagctc ggagagctcg agcaccgtca tgttggagat cgcgtcgagg
     6961 atctcttcct tggtcgccat gtctgtcact cctgttcctg ggcggcgtcg tcggcgtcag
     7021 cctcggccgc gtccccgccc tcggcggggg tctggtcctg atcttcggtg tcggtcgatg
     7081 cgtcggcctg tgccggcgcc tcggcctcag cggtgtccgc tggatctgcc tcggcagtgg
     7141 gttcctgggt ggctgcctca tcggcagcgg gctcggtggg ttcgtcggcg accgacgcgg
     7201 gtgcgtcgct cgctccgccg gcctcgatca gcgcctgcag tgcgtacgcc atcttctgag
     7261 ggatcgcgtt gagcaggctg gcgaagttct gcatgggcgc agccatgcca ccggcgagac
     7321 gagcgagcag ctcctcccgg ggtgcgacct ccgccagacg cgacaacgcc gcctggtcga
     7381 gcagcgagtc gcccagcacg ccgcccttca tgaccaacag cgggttggac ttggcgaacg
     7441 acttgagggc cttcgccacc gtcacgacgt cgcccggcgt gccgtcggcc ttgctgccga
     7501 cgaacgtgag cgccgtcggt ccgagcaggt gctcgtcgag ctcgaggtcc aacgacgccg
     7561 tcgcccgcct gaccagggtg ttcttgtaca cgtggtactc gccaccggcc tcgcgcagcg
     7621 aggtccgcag gtcctgcagg tcccgaaccg tcagaccgcg gtactcggtg atgatcacgg
     7681 cgtcggcggt ctggaacttg tccttgacct cctccaccac ggccaccttc tccggcctcg
     7741 ggttctccat ctgacacctc cttccgtctg tctcggggac cgctggcgcg gtccggttcc
     7801 ggggttgcgt gccgcaaccc tcctgggtcg ggatccggcg cccgcaccag cggacgcctc
     7861 ctcgaccgac cgcaggggac ggtgttcgga tcaggtgtcc acctacccag gtgggccggt
     7921 gggtgtcccg cccccggagg cgatcgcctg cggaggtccg accgggtcgc tccgggcgga
     7981 tggtggacgc ccggggcccg ttgtgcaccg tccggtgcgc cgtggggtct ctggcgagtg
     8041 tcagatctcg gtgtcccccg gaggggaccg gtcgatgcta ccggagcggt ccgccccggc
     8101 accagcgccg aactcggccg ggcgggccgg cctcagccct cgaggcggtg gacgtcgagc
     8161 tgcacgcctg ggcccatggt cgagctgagg gtgaccttgc ggatgtagcg acccttggcc
     8221 gccgagggct tggccttgat cagctcgtcg atgatggcgc cgaagttggc gaccagcgcg
     8281 gccgcgtcga agctcgcctt gccgatggcg acgtgcacgt tgccgttgcg gtcggtgcgg
     8341 tactcgacct tgccgccctt gaact
//
LOCUS       DAOQOD010000133         8361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3137_length_8361_cov_10.727546, whole genome shotgun sequence.
ACCESSION   DAOQOD010000133 DAOQOD010000000
VERSION     DAOQOD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3137_length_8361_cov_10.727546"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLZ72_11970"
     CDS             <1..941
                     /locus_tag="PLZ72_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37060.1"
                     /translation="TATAMSEEAEVEQAARTLLMARRTAEQMEADARGQAQNLLAEAK
                     TRADRQLAEAQAEAEELIRRARTQADSEFADRRERASEEVQVLESRRSQLADVITQLE
                     ARLAGYREDLTRTAEELTLLASDPDRLGARPTMSIPPDEVISQRGGPTAASAPTKEPE
                     PSAPRVDEVVEASTVEPEAPVVAEPAAPSGGSTTAASAPTAERVVDFDKGADGGPATE
                     AVDAASGDWGPGSWSRLQASGSDEQEETISLADEPSSRTSPHIIAEPVTGSGRDRYLQ
                     DLDDAVNVADDSDDEAMTAFFEGSGDSRNRRFGWRR"
     gene            1229..>1475
                     /locus_tag="PLZ72_11975"
     CDS             1229..>1475
                     /locus_tag="PLZ72_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37061.1"
                     /translation="MATKRAGSTTASSKKAAPAKKAAPAKKAAPAKKAAPAKKAAPAK
                     KAAPAKKAAPAKKAAPAKKAAPAKKAAPAKKAAPAKKA"
     assembly_gap    1476..1575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1623..1722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1723..2275
                     /locus_tag="PLZ72_11980"
     CDS             <1723..2275
                     /locus_tag="PLZ72_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNA37062.1"
                     /translation="KAAPAKKAAPAKKAAPAKKAAPAKEAAPAKEAAPAKKAAPAKKA
                     APAKKAAPAKKAAPAKNPFDKAFLDEQRALLLEERATLHEQAESLRAEAEALAFDREP
                     GDVQFDDESGEGDTLAVERDFDLARAAQARQTIDEIDAAIERIGKGTYGICEYSGDPI
                     PKERLRAIPWARERVEYKTRSFR"
     gene            2335..2931
                     /gene="lspA"
                     /locus_tag="PLZ72_11985"
     CDS             2335..2931
                     /gene="lspA"
                     /locus_tag="PLZ72_11985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNA37063.1"
                     /translation="MSSTRRWSIVVGAALVVLGLDQLTKAWAVDRLKDQLPIDVVWTL
                     RFNYAENTGMAFSTGSGAGRWISLVVLAIVVALVLLARSVRSTAALVLIGVVIGGAFG
                     NLFDRIFRAQAGFLSGAVVDWIDVQWWPIFNIADAAVVVGGILIAIFLLREPVEDEPV
                     PAGAEPDTEPVSTDWAGGDAGVRPSSEDTTGTGEPGAR"
     gene            2931..3962
                     /locus_tag="PLZ72_11990"
     CDS             2931..3962
                     /locus_tag="PLZ72_11990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNA37064.1"
                     /translation="MGDGPAFLDETVPDALDGERIDRALATLVPCSRSEAAEVIGSGG
                     VSIDGTTISKPSTRVAVGQRLVLAVGPTRRETVVAADPDVEFAVVHADDDIIVVDKPA
                     GLVVHPGPGHRGSTLVHGLVARFPDLDPAGGVIVGEPERPGLVHRLDRGTSGLLVVAR
                     TPDAYDSLVHQLSTHAVERTYTALVRGHPEHPHGVIDAPIGRSRRDPLRMTVAADGRP
                     SRTHYRLEHTFSTDASDRPTPVSAVTCDLETGRTHQIRVHLASIGHPVVGDPLYGGPD
                     RRPHVARPFLHARRLSFVHPGTGDDVAYEAALPADLAAVLASLRPRWEGIPGDDSDGG
                     SDGGIGHVV"
     gene            3995..4432
                     /locus_tag="PLZ72_11995"
     CDS             3995..4432
                     /locus_tag="PLZ72_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNA37065.1"
                     /translation="MRALVIHESLTGNTRTAARTMVAELQQEGWEASECSSRQVDLAA
                     LQQSDVVIVGTWVDGLVLFGQRPGGAGKLAKLPLLGGKPTYVFVTYAINPGRTLEKLT
                     AEIEDRRGDVRGGMVIRRGQLTEGAVEFARRVIDASAELAADA"
     gene            complement(4482..4919)
                     /locus_tag="PLZ72_12000"
     CDS             complement(4482..4919)
                     /locus_tag="PLZ72_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNA37066.1"
                     /translation="MKVSTRGDYACRALLSLSLHGDDGPTSVRDIAERTGLPQPYLEQ
                     ILLALKGAGLVRSKRGVGGGYILARDPAEIDLAQIVSAVDGPIQAGDFGQPHTDGACD
                     HEGQCVLLTVWHDVGGHMRRLLEGYTLAHLAEVARGERPWPGA"
     gene            5019..6146
                     /locus_tag="PLZ72_12005"
     CDS             5019..6146
                     /locus_tag="PLZ72_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNA37067.1"
                     /translation="MTSKEPAVVDLPVLPDYEGACTCNVMTPLVEDPDRVPAWFPDGV
                     VGADQVVLWVLDGLGWEQMQDRIGMLRGLSSMNAAPITTIAPTTTATALTSIATGLPP
                     GEHGVIGYRMSVEGDDILNVLRWTTSKGDARESIPPESIQHELAFEGHRPPAVVRAEF
                     RDTGFTRAHLGGCRHVPYRMPSTLVAEILHQVHTGEPFVYAYYDGIDKVAHEYGLGMV
                     YDAELVFVDRMVEYLLHELPPGVALVITSDHGQVDVGERVFPPHPEVLSHVRHQSGEA
                     RFRWLHAHPGAATSLYEAAQRHHGHEAWVVTREQVVDDCWFGPKVLPGALARLGDVAL
                     VAREPIAYEDPADTGPFHLLGRHGSMTSAEVMVPLLVARTD"
     gene            6202..6780
                     /locus_tag="PLZ72_12010"
     CDS             6202..6780
                     /locus_tag="PLZ72_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2587 domain-containing protein"
                     /protein_id="HNA37068.1"
                     /translation="MADEVTTPPEVLEPDAVQPPPASPGGIAGGAAPLVGGPHADGAG
                     DGGDSGGGDEDVVPDVNSPAKIIRIGAMVKQLLEELRSTSLDEPSRSQLRDIYERSIA
                     QLETALSPELASELSELSFDFPEGTVPSEVELRMAQSQLVGWLEGLFHGIQAALVAQQ
                     MSARQQLEGMRGQLMQGPNGPQPAPGPGPGYI"
     gene            7170..8303
                     /locus_tag="PLZ72_12015"
     CDS             7170..8303
                     /locus_tag="PLZ72_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37069.1"
                     /translation="MTAVLLGAAAACTPPPEPPPACPARQQVVLEPTDPVAFSLARAV
                     SPNGAHMVLSRVLGSDLVLSLRTVQPGSPSTVVGSLPTAPTDRRVLVGVDDAGSRVVF
                     GREGRAATPADPPSVLHRWSASTGTVTDIAAPTVAAPPPGVAYPANARAISADGRRIV
                     WTQSFREGPEPHIWHRMVTVTDAGTDAVIAQWESPGWVLTGSPWVSSGGRQTLDFGTM
                     VSLDTGQRTDLGPDIAAASAAFPGPVLGVAAVSDDGVHLVLQGDDPFGNPRVLTTVLW
                     DRSTGTGVKAPDGAWGYPPAIVGVSNSGEAVQAVPYNYRHELADIGVLTLDGRRIPIG
                     TAALRPWPDDYSEIYAVSTPDLRTVLLTFELNIGYTLVAQRCS"
BASE COUNT         1172 a         2990 c         2916 g         1083 t
ORIGIN      
        1 cgaccgccac ggccatgagc gaggaggccg aggtcgaaca ggccgcccgg acgctgctga
       61 tggcgcgtcg caccgccgag cagatggagg ccgacgcgcg cggtcaggcg cagaacctcc
      121 tggccgaggc caagacccgg gccgaccgcc agctcgccga ggcccaggcc gaggccgagg
      181 agctgatccg tcgggcccgc acccaggccg acagcgagtt cgccgaccgt cgcgagcgcg
      241 cctcggaaga ggtgcaggtg ctcgagagcc gtcgttcgca gctagcggac gtcatcacgc
      301 agctcgaggc ccgcctggcc ggctaccgcg aggacctgac ccgcaccgcc gaggagctga
      361 cgctcctggc gtcggacccc gaccgtctcg gtgcccgacc gaccatgtcg atcccgcccg
      421 acgaggtgat ctcccagcgg ggcggcccga ccgcggcgtc ggcaccgacg aaggagcccg
      481 agccgtccgc acctcgagtc gacgaggtcg tcgaggcgtc gacggtggag ccggaggccc
      541 ccgtcgtcgc cgagccggcc gctccctccg ggggcagcac cacggcggcc tccgccccga
      601 ccgcggagcg ggtcgtcgac ttcgacaagg gcgcggacgg cggacccgcg accgaagcgg
      661 tcgacgcggc gtccggcgac tggggtcccg ggagctggtc acggctccag gcctcgggat
      721 cggacgaaca ggaggagacg atctccctgg ccgacgagcc gtcgagccgc acgtcgccgc
      781 acatcatcgc cgaaccggtg accggctcgg gtcgtgaccg ctaccttcag gacctcgacg
      841 acgcggtcaa cgtggccgac gactccgacg acgaggcgat gacggcgttc ttcgaggggt
      901 cgggcgactc ccgcaaccgg cgcttcggct ggcgacgctg aaccccgcat ccacggtcgt
      961 cgccgacctc ccgcgccccg acggcgacga gatcgcggcg gtcgccccca ccgctgcggc
     1021 cgcggccgac gtcgacgacg gatgccgcct gatcgagctc cccggagtgg gcgtcgtcct
     1081 gcgcccggtg ctgcgcggcg gccgggcggt gtggggcgtg ctggctcggg ggttggtcgc
     1141 gccccggtgg ggagcgccgc ggctaaggtt ccccgaccat ctgggcgccc ggcccagctc
     1201 atcgacccgt cagggaagga ggccgatgat ggcgaccaag cgcgccggat cgaccaccgc
     1261 atcgtccaag aaggcggctc cggccaagaa ggctgctccg gccaagaagg ctgctccggc
     1321 caagaaggct gctccggcca agaaggctgc tccggccaag aaggccgctc cggccaagaa
     1381 ggccgctccg gccaagaagg cggcaccggc caagaaggcg gcaccggcca agaaggccgc
     1441 accggccaag aaggccgcac cggccaagaa ggcggnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnngcacc ggccaagaag gccgcaccgg ccaagaaggc cgcaccggcc
     1621 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaggcgg caccggccaa
     1741 gaaggcggca ccggccaaga aggcggcacc ggccaagaag gcggcaccgg ccaaggaggc
     1801 ggctccggcc aaggaggcgg ctccggccaa gaaggcggca ccggccaaga aggccgctcc
     1861 ggccaagaag gctgcaccgg ccaagaaggc tgcaccggcc aagaacccgt tcgacaaggc
     1921 cttcctcgac gagcagcgag ccctcctcct ggaggagcgg gcaaccctgc acgaacaggc
     1981 cgagtcgctg cgggccgagg ccgaggccct ggccttcgac cgcgaaccgg gcgacgtcca
     2041 gttcgacgac gagtcgggcg agggcgacac cctggcggtg gagcgcgact tcgacctggc
     2101 ccgtgccgcc caggcccgcc agaccatcga cgagatcgac gccgccatcg agcggatcgg
     2161 caagggcacg tacgggatct gcgagtactc gggggacccg atccccaagg agcggctccg
     2221 ggccatcccg tgggcgcgtg agcgcgtcga gtacaagacg cgcagcttcc gctgacgccc
     2281 gggcgccgtt tccgcgtcga tgcgccggtc ccgaccatga cgaccccgtc caccgtgagc
     2341 agcacgcgcc gctggagcat cgtcgtgggt gccgcgctcg tggtgctggg cctcgaccag
     2401 ctcaccaagg cgtgggcggt cgatcggctg aaggaccagc tcccgatcga cgtcgtgtgg
     2461 accctccggt tcaactacgc cgagaacacc gggatggcgt tctcgaccgg gtcgggggcc
     2521 ggtcggtgga tctccctcgt cgtcctggcg atcgtcgtcg ccctggtgct gctggcacgc
     2581 agcgtccgct ccaccgcggc gctcgtgctg atcggcgtcg tgatcggcgg cgccttcggc
     2641 aacctgttcg accgcatctt ccgggcccag gcgggcttcc tgtcgggcgc tgtcgtcgac
     2701 tggatcgacg tccagtggtg gccgatcttc aacatcgcgg acgccgccgt cgtcgtcggc
     2761 gggatcctca tcgccatctt cctgctgcgc gagccggtcg aggacgaacc cgtcccggcc
     2821 ggcgcggagc cggacacgga gccggtctcc actgactggg ctggcggcga cgccggcgtc
     2881 cgaccctcct cggaggacac gaccggcacg ggcgagcccg gggcccggtg atgggcgacg
     2941 gtcccgcctt cctggacgag accgtgcccg acgccctcga cggcgagcgg atcgaccgtg
     3001 cgctggccac cctggtgccc tgctcgcgct ccgaggccgc cgaggtgatc ggttccggcg
     3061 gggtgagcat cgacgggacg accatctcga agccctcgac cagggtggcg gtcggccagc
     3121 ggctggtgct ggccgtcggt ccgacccgcc gcgagacggt cgtggccgcg gaccccgacg
     3181 tcgagttcgc cgtcgtccac gcggatgacg acatcatcgt cgtcgacaag cccgcgggac
     3241 tcgtcgtgca ccccggtccg ggtcaccgtg gctccaccct ggtccacgga ctggtcgccc
     3301 gcttccccga cctcgacccc gccggcgggg tgatcgtcgg cgagcccgag cggccggggc
     3361 tcgtgcaccg cctggaccgg ggcacctcgg gcctcctggt ggtcgcccgg acccccgacg
     3421 cgtacgactc gctggtgcac cagctgtcga cccacgcggt cgaacgcacc tacacggccc
     3481 tggtgcgggg acaccccgag caccctcacg gcgtgatcga cgccccgatc gggcgctccc
     3541 ggcgtgaccc actgcggatg accgtcgccg cggacggtcg gccgtcgcgc acccactacc
     3601 gactcgagca cacattcagc accgacgcga gcgaccgtcc cactccggtc tccgcggtga
     3661 cctgtgacct cgagaccggc cggacccacc agatccgcgt gcacctggca tcgatcggcc
     3721 acccggtggt cggcgatccg ctgtacggcg ggccggatcg gcgacctcac gtcgcccgcc
     3781 cgttcctgca cgcccggagg ctgtcgttcg tccatccggg gaccggcgac gacgtcgcct
     3841 acgaggcggc gcttcccgcg gatctcgccg ccgtcctcgc atcgctgcga cctcggtggg
     3901 aggggatccc gggcgacgac tccgacggtg gctccgacgg tggcatcggc cacgtcgttt
     3961 gacccgacga cacctcacac aggagcatga gcccatgcgc gccctggtca tccacgagag
     4021 cctcaccggc aacacccgga ccgccgcccg gacgatggtc gccgagctgc agcaggaggg
     4081 ctgggaggcc agcgagtgct ccagtcgcca ggtcgacctg gccgcgctgc agcagtccga
     4141 cgtggtgatc gtcgggacct gggtcgacgg cctcgtcctg ttcggtcagc gtccgggcgg
     4201 ggccggcaag ctggccaagc tcccgcttct cggcggcaag cccacctacg tgttcgtgac
     4261 ctacgcgatc aaccccggtc ggacgctcga gaagctgacc gccgagatcg aggaccgacg
     4321 gggcgacgtg cgcggcggca tggtcatccg gcgcggccag ctcaccgagg gcgccgtcga
     4381 gttcgcccgc cgggtcatcg acgcctccgc cgagctcgcc gccgacgcct gacccgcgtc
     4441 accgctgtcg cgcaggcggg ctcaggcggg cccagtcggg cttaggcgcc cggccagggc
     4501 cgttcgcccc gggccacctc ggccaggtgc gccagggtgt agccctcgag cagtcggcgc
     4561 atgtggccgc cgacgtcgtg ccacacggtc agcagcacgc actggccctc gtggtcgcag
     4621 gcgccgtcgg tgtggggctg tccgaagtcg ccggcctgga tcgggccgtc caccgcggag
     4681 acgatctggg cgaggtcgat ctcggcgggg tcgcgggcca ggatgtagcc gccgccgacc
     4741 ccgcgcttgg agcgaaccag gcccgcaccc ttgagggcca gcaggatctg ctcgaggtag
     4801 ggctggggca ggccggtccg ctccgcgatg tcgcgcaccg aggtcggtcc gtcgtcgccg
     4861 tgcagcgaca acgacagcag ggcgcgacag gcgtagtcgc cgcgggtgga gaccttcacg
     4921 tcggccatcg taggcacccg cagctcaccg gtccgtcccg gtcggaccgg tggtgaccga
     4981 cacgccctcg gtgctcgggg accgctaact tggagtcggt gaccagcaag gagcccgcgg
     5041 tggtggacct ccccgtgctg cccgactacg agggcgcatg cacgtgcaac gtcatgaccc
     5101 cgttggtcga ggacccggat cgggtgccgg cctggttccc cgacggcgtc gtcggcgcgg
     5161 accaggtggt gctgtgggtc ctcgacggcc ttggctggga gcagatgcag gaccgcatcg
     5221 gcatgctgcg gggcctgtcg tccatgaacg ccgctccgat caccacgatc gcgccgacca
     5281 ccacggccac cgcgttgacc tcgatcgcca ccgggctgcc gccgggcgag cacggggtga
     5341 tcggctatcg catgtcggtc gagggcgacg acatcctgaa cgtgttgcgc tggaccacct
     5401 ccaagggcga cgcccgggag tccatcccgc cggagtcgat ccagcacgag ctcgccttcg
     5461 agggacaccg ccccccggca gtggtgcgcg ccgagttccg tgacacgggc ttcacccggg
     5521 cgcacctggg gggctgtcgc cacgtcccgt accggatgcc gtccacgctg gtggccgaga
     5581 tcctgcacca ggtccacacg ggcgagccgt tcgtctacgc ctactacgac ggcatcgaca
     5641 aggtggccca cgagtacggg ctcgggatgg tctacgacgc cgagctcgtg ttcgtcgacc
     5701 gcatggtcga gtacctgctc cacgagctgc cccccggtgt ggcgctggtg atcacgtccg
     5761 atcacggcca ggtcgacgtg ggtgagcggg tgttcccgcc gcaccccgag gtgctcagcc
     5821 acgtccgcca ccagtccggc gaggcccggt tccgctggtt gcacgcccat ccgggcgccg
     5881 ccacctccct gtacgaagcg gcccagcgcc accacggcca cgaggcgtgg gtcgtgaccc
     5941 gcgagcaggt cgtcgacgac tgctggttcg ggcccaaggt cctgcccggg gcgctggccc
     6001 gcctcggcga cgtcgccctc gtcgcccgcg agccgatcgc ctacgaggac cctgccgaca
     6061 ccgggccgtt ccacctgctc ggccggcacg gctccatgac ctctgccgag gtcatggtcc
     6121 cgctgctcgt cgcccgcacg gactgatcgg gccgagccgg tcgggccggc caggaacggg
     6181 tcgcggggta gggtcgaacc catggctgac gaagtgacca cgcctcccga ggtgctcgaa
     6241 cccgacgccg tccagccgcc gccggcgtcg cccgggggca tcgccggcgg ggcggcaccg
     6301 ctggtgggcg ggccccacgc cgacggtgca ggtgacgggg gcgactcagg tggtggcgac
     6361 gaggacgtcg tgcccgacgt caactcaccg gccaagatca tccgcatcgg ggccatggtc
     6421 aagcagctgc tcgaagagct ccgcagcacg tcgctggacg agccgtcccg gagtcagctg
     6481 cgcgacatct acgagcgctc gatcgcccag ctcgagacgg ccctgtcgcc cgagctcgcg
     6541 tcggagctca gcgagctgtc cttcgacttc cccgagggca cggtgccctc cgaggtcgag
     6601 ctccgcatgg cccagtcgca gctggtcggc tggctcgagg gcctgttcca cgggatccag
     6661 gcggcgctgg tcgcccagca gatgtcggcc cgccagcagc tcgaggggat gcggggtcag
     6721 ctgatgcagg gcccgaacgg tcctcagccc gcccccggtc cgggccccgg ctacatctga
     6781 ggccgtcggc gcccgccgcg cagccccgac ggttcccgaa cttgtagccc acggtcgccg
     6841 tggtggccgc tcacctacaa gttcggcggc ccccggcgaa cttgtagtcc accgtcgctg
     6901 tggtgaccgc tgacctacaa gttcggcggc tcccggcgaa cgtgtagccc acggtcgctg
     6961 tggtgaccgc tgacctacaa gttcggcggg tgtcgaccgt gtgacccagt ggggagtcgt
     7021 ccccagtgat tcgatccggc gcactgaata cgatccgcgg cagggcgacg agggaggttc
     7081 ccatgcgctg gatcgaccga actgaagctg cggcagcggg tgctgtgggg ggtggacaac
     7141 gacgggttcg ggcgtcgttg gccgtcgggt tgaccgcagt tctgctggga gccgcggcgg
     7201 catgcacacc cccgccggag cctccgccgg cctgcccggc ccgccagcag gtggtcctgg
     7261 agccgaccga cccggtggcg ttctccctcg cccgggccgt gtcgcccaac ggcgcccaca
     7321 tggtcctgtc ccgggtgctg ggctcggacc tggtgctgtc gctgcggacg gtgcagcccg
     7381 gatcgccctc gacggtggtg ggctcgctgc cgacggcccc gaccgaccgt cgcgtcctcg
     7441 tgggtgtgga cgacgccggg agccgggtcg tgttcggacg cgagggaaga gcggccacgc
     7501 ccgccgaccc gccgagcgtg ctgcaccgct ggtccgcgag caccggaacg gtgaccgaca
     7561 tcgcggctcc gacggtggcg gccccaccgc ccggggtggc ctacccggcc aacgctcggg
     7621 cgatcagcgc ggacggacgt cggatcgtgt ggacgcagtc cttccgcgag ggtccggagc
     7681 cgcacatctg gcatcgcatg gtgaccgtga ccgacgcggg gaccgacgcc gtgatcgccc
     7741 agtgggagtc accggggtgg gtgctgaccg gcagtccctg ggtgtcctcg ggcggacgcc
     7801 agaccctcga cttcgggacg atggtgtcgc tcgacaccgg ccagcgcacc gacctgggtc
     7861 ccgacatcgc cgcggccagc gccgcgttcc cgggcccggt actcggagtg gccgccgtca
     7921 gcgacgacgg agtgcacctc gtcctgcagg gcgacgaccc gttcgggaac ccccgcgtgc
     7981 tcacgacggt cctctgggat cgttcgaccg gcaccggggt gaaggcgccg gacggtgcgt
     8041 ggggctaccc gccggcgatc gtcggtgtgt ccaactcggg cgaggccgtg caggccgtgc
     8101 cgtacaacta ccggcacgag ctggccgaca tcggagtgct gaccctcgac ggtcggcgga
     8161 tcccgatcgg gaccgccgcg ctccggccgt ggccggacga ctacagcgag atctacgcgg
     8221 tcagcacacc ggatctgcgg accgtactgc tgaccttcga gttgaacatc ggctacacgc
     8281 tggtcgccca gcgctgctcc tgaactgccc gccagccatc cggctgcgtt gttgtagctg
     8341 gcaggtcgcc cggcgacctg t
//
LOCUS       DAOQOD010000134         8188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3244_length_8188_cov_12.278987, whole genome shotgun sequence.
ACCESSION   DAOQOD010000134 DAOQOD010000000
VERSION     DAOQOD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3244_length_8188_cov_12.278987"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLZ72_12020"
     CDS             <1..606
                     /locus_tag="PLZ72_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37070.1"
                     /translation="ILDGVDLAVERGEVVALTGENGAGKSTLMRICAGLERADSGTVT
                     VTGRIGYCPQIPGLFELLTAEDHLVMFGRGAGIGRAESLERGRAILAEFDYPLHERSV
                     TRDLSGGTRQKLNLALALLVDPTVLLLDEPYQGFDRGTYVNFWDHCARWRDQGRAVVV
                     VTHMLAELERADRVVELPAHPSRAVDAAAPGPRRRRSGRSR"
     gene            603..1280
                     /locus_tag="PLZ72_12025"
     CDS             603..1280
                     /locus_tag="PLZ72_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37071.1"
                     /translation="MKGAAGRIGIMAEMHGRDLTRRHGALGLLVALPLCFYLASMGSG
                     VRAVVAGGVGMAFSVSGATLFSLLSSEEVDQRLVLGGYRPLDLLLGRLAFLAPLGLAV
                     AALFCALMLPVSQPDRPWLAWLGVSAVAVQSIAFGLAVGAVVPRELEGTLVLIGVVGM
                     QLAVEPDNAVAKVLPFYGPQQLIESSLSPAGGPILWPLVLTAAYGVVLMVVARAFLGH
                     RLEVVRQ"
     gene            complement(1289..>1861)
                     /locus_tag="PLZ72_12030"
     CDS             complement(1289..>1861)
                     /locus_tag="PLZ72_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37072.1"
                     /translation="EYVAITGRSGCGKSTLMHLLAALDHPDSGSLRVNGRDITHPDHV
                     NRYRRHDVGLVFQMHNLLPHLDARSNVAVAMLGTHTSRSDRRERADELLEMVDLADLG
                     HRRPPEMSGGERQRVAIARALANRPGLLLADEPTGSLDSGAVERVLGILEQLRSEEDV
                     TIVVVTHDDHVADAADRVIRMDDGRVVDAL"
     assembly_gap    1862..1961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1965..3083)
                     /locus_tag="PLZ72_12035"
     CDS             complement(1965..3083)
                     /locus_tag="PLZ72_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37073.1"
                     /translation="MSFIWLIGHNVWSKKVRSMLTAAAVAVGVLVVVALGIVTDSIRT
                     TAAGVLKVGAADFTISQKGLSDILESALTEQQLTRVQKTPGVKSAIGVLLDTEALDKD
                     HPLVVEVGIRPEDLAPFGVRLAAGREFTAEAADEIMVGVRLAQDIGVGPGDTLHIGGS
                     DKTVVGVFNTGNTFGDSTVMFPLVPFQAYERQPGGLSLFFVKVDKGTQVKALEQRIER
                     DNPLLLGIRNLLEFGRADRGYQLITGADKAATIVAVAVGAIVVANAMLLSLVERYREF
                     GVLRAIGWSRRRLIALIFGEAAIIAFIGACLGVVIAFIAVWVLADLPDLAGILKPTFE
                     AWVFGRALVTATAVTALGALYPALRAARLSPQEAMRRE"
     gene            3211..5361
                     /locus_tag="PLZ72_12040"
     CDS             3211..5361
                     /locus_tag="PLZ72_12040"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNA37074.1"
                     /translation="MAEARPDDVIRIDAGGWGLSWAPSADGTLHQVGLGPAGAAATRD
                     VDAHWYPRAFPTLDGTDPFRPPALHVVHADGTLSTRLALDDVDRSDAADGSGEHVVLT
                     TVDELFDLTVEHHLRTHPDSGVLEQWVEVVNAEDGPVTLLSHDSIAPFLMVPDDAEYV
                     QFGGAGWADEWRWSAGRPVIGTTVLGSLGGVQPHLQRSPCLLLSPTGPFDVDAAGAPA
                     QDRGEVIGLSVAWGGNSHVSLDLRPRAEPGAPGELRVRAGAHALGAPYRLDPGTRMVL
                     PTVAWVWSSAGRDGVTEAFHRWSRARVLRDPDRRRPLVVNNWEATYFDFDEHRIVGLI
                     DRAAELGADVFLLDDGWFGTTRPRDDDTQGLGDWDVDRRKLPGGLGPLTDAAAERGIR
                     FGIWVEPEMVNPLSDLYDDHPEWVARDVREPREHRQQLYLDPLRPEVRDFEVDVVGQT
                     LRSAPGTSYVKWDANRPVTDPGSATLPADRQANVWFDQVGATWDVMARVAAAHPDVEL
                     MLCASGGGRVDHGSLRWFHEFWTSDNTDPVARVRMQWACSHFFPGAVMAAHVTRWGER
                     PMAFASAVALSGRFGIDLDLETTTPSEDDVLSRAVEWARRTQDVVQQGTVRRYVSPVD
                     GADRSRAAFSVTLGDRTVVFAYQLEESTSTPPVIRLRGLPRAGTYRVRTTDLHGPAEE
                     TTSTDDELDRGIEWPLDRPLSARLWEIEPARRTA"
     gene            complement(5387..6067)
                     /locus_tag="PLZ72_12045"
     CDS             complement(5387..6067)
                     /locus_tag="PLZ72_12045"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HNA37075.1"
                     /translation="MTLTGYALAGEDPSVAVAPTLVAGTLLVAFFVVARRPGLLRAPA
                     RIAVGSWSGIRRRIRGRPAREPGSLHDLHDLHDLHDLHDQADRAADRFVASFGAVHAG
                     LGRWSAAWALQVLSWFGEFVSLVAAVAATGGSIPSDPATWGWLLAVYGTAQLAGAIPI
                     IPGGAGQVEAALVVGLTATGTDASTALAASVAFRLMSHWLVVPIGWVCVAALRRQGAD
                     VPRRPTAA"
     gene            complement(6064..6510)
                     /locus_tag="PLZ72_12050"
     CDS             complement(6064..6510)
                     /locus_tag="PLZ72_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNA37076.1"
                     /translation="MEGGDGTAGSVRTQGRRRGLRVGVAVLAAAGLVWFLVVERDQVL
                     AAVDLLVGADPVWIAVGSVSSILSIVVFAAVRSVLVGASGARFPLGRATTASFASGAI
                     ATTLPAGGAIATGYMVQRYREAGAAAAQRRGPRSPPGSSRRRFLCS"
     gene            6586..7326
                     /locus_tag="PLZ72_12055"
     CDS             6586..7326
                     /locus_tag="PLZ72_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNA37077.1"
                     /translation="MTATQRPRTPRSSAPPPGPNPVNRGLILIAVGVVLAVILLVKAG
                     GVGFDSSSTDVAIGEGEEKVTTTTEATTTTVAEQAPQTVQVVAANGSGASGMAAKAGQ
                     VLAQNGYTQVVTTNALQPVTASSVLYATGYDANARSIAKMFGLAESVVQPLAAGTQLA
                     KEQPPTTGVVVMIGPDLTQTLSGVGATATTVAGSSGGVTTTVKSGSGGTTNTTVKSGT
                     AGATTTTVKATSGAGAVTTTVKAATTTR"
     gene            7336..8103
                     /gene="otsB"
                     /locus_tag="PLZ72_12060"
     CDS             7336..8103
                     /gene="otsB"
                     /locus_tag="PLZ72_12060"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNA37078.1"
                     /translation="MDRSSLLEPFHTHAADALVAFDFDGTLAPIVEDPDAASPMAGAV
                     SALERLAARVGEVAVVSGRPLDFLERWFPEPSAVTLVGLYGLESRRQGDRADHPSAGV
                     WREVVSDLAERARRHGPDGMDVEPKGLSITLHYRRRPDVEPSVRDWAERAAGATGLSV
                     RSAKMSVELHPPIDEDKGTVLHRLAGFHTGPVLFAGDDVGDVPAFDALGGLAAAGRPV
                     LAIVVDGPEVPSTLRRRADLLVDGPAEVADIVLSLAG"
BASE COUNT         1120 a         2931 c         2932 g         1105 t
ORIGIN      
        1 atcctcgacg gggtggacct ggcggtggag cggggagagg tcgtcgctct gaccggtgag
       61 aacggcgccg ggaagtcgac gctcatgcgg atctgcgccg gactggagcg tgcggactcg
      121 gggacggtga ccgtgaccgg acggatcggc tactgcccgc agatccccgg tctcttcgag
      181 ctgctcaccg ccgaggacca cctggtcatg ttcggacggg gtgccggcat cggccgggcc
      241 gaatccctgg aacgaggtcg ggcgatcctg gccgagttcg actatccgct ccacgagcgc
      301 agcgtcaccc gcgacctgtc cggcggaacc cgccagaagc tcaacctcgc gctggccctg
      361 ctggtcgacc cgacggtgct gctgctcgac gagccctacc agggcttcga ccgtgggacc
      421 tacgtgaact tctgggacca ctgcgcgcgc tggcgggacc agggtcgggc cgtggtggtc
      481 gtcacccaca tgctcgccga gctcgagcgc gccgaccggg tcgtcgagct gccggcgcat
      541 ccgtcgcggg cggtggacgc cgccgccccg ggcccgcgcc ggagacggtc ggggaggtcc
      601 cggtgaaggg ggccgccggg cggatcggga tcatggccga gatgcacggt cgggacctca
      661 cccgtcgtca cggtgcgctg ggcctgctcg tcgcgctgcc gctgtgcttc tacctggcgt
      721 cgatggggtc cggggtgagg gccgtcgtcg ccggcggggt cggcatggcg ttctcggtgt
      781 ccggagccac cctgttctcg ctgctgtcgt cggaggaggt cgaccaacgg ctcgtcctcg
      841 gcgggtaccg gccgctcgac ctgctcctgg ggcgtctggc cttcctggcc ccgctcgggc
      901 tggccgtcgc cgccctcttc tgcgcgctca tgctgcccgt gtcccaaccg gaccgaccgt
      961 ggctggcgtg gctcggggtg tccgccgtcg cggtccagtc gatcgcgttc gggctcgcgg
     1021 tcggggccgt cgtccctcgc gagctcgagg ggacgttggt gctcatcggt gtggtcggca
     1081 tgcagctcgc cgtcgagccg gacaacgcgg tggccaaggt cctgccgttc tacgggccgc
     1141 agcagctgat cgagtcgtcg ttgagtccgg ccggcggccc gatcctgtgg ccgctggtgc
     1201 tcaccgcggc ctacggcgtc gtcctcatgg tcgtcgcccg ggcgttcctg ggacatcgcc
     1261 tggaggtcgt ccggcagtga cggtccgctc agagggcgtc gaccacccgg ccatcgtcca
     1321 tgcggatgac ccgatcggcg gcgtcggcga cgtggtcgtc gtgggtcacg accacgatcg
     1381 tgacgtcctc ctcggaccgg agctgctcga ggatcccgag cacccgctcc actgcgccgc
     1441 tgtcgagcga acccgtcggc tcgtcggcga gcagcaggcc gggccggttg gccagcgccc
     1501 gggcgatggc cacccgctga cgttcgccgc cggacatctc aggggggcgc cgatgaccca
     1561 ggtcggccag atcgaccatc tcgagcagct cgtcggcccg ctcccgacga tcgctccgag
     1621 aggtgtgggt cccgagcatg gcgaccgcga cgttggagcg ggcgtcgagg tggggcagga
     1681 ggttgtgcat ctggaacacc agcccgacgt cgtggcgtcg gtagcggttg acgtggtcgg
     1741 ggtgggtgat gtcgcggccg ttgacccgaa gcgatcccga gtcggggtgg tcgagcgcgg
     1801 cgaggaggtg catcagcgtc gacttgccgc acccggaccg gccggtgatc gcgacgtact
     1861 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctctcattc acgacgcatc
     1981 gcctcctggg gtgagagtcg ggcggcgcgc agcgccgggt acagggcgcc gagcgcggtc
     2041 acggccgtgg cggtcaccag cgcccgtccg aacacccacg cctcgaaggt cggcttgagg
     2101 atgccggcca ggtcgggcag gtcggccagg acccacacgg cgatgaacgc gatcaccaca
     2161 cccaggcagg cgccgatgaa ggcgatgatc gctgcctcgc cgaagatcag cgcgatcagt
     2221 cgccgtcggg accagccgat cgcccgcagc acaccgaact cgcggtagcg ctcgaccagc
     2281 gacaggagca tcgcgttggc caccacgatg gcgcccaccg cgacggcgac gatggtagcg
     2341 gccttgtcgg cgccggtgat cagctggtag ccgcggtcgg cgcgaccgaa ctcgagcagg
     2401 ttccggatgc cgagcagcag cgggttgtcc cgctcgatcc gctgctcgag cgccttcacc
     2461 tgggtgccct tgtcgacctt cacgaagaac aacgacaggc cgccgggttg gcgttcgtag
     2521 gcctggaacg ggacgagcgg gaacatgacg gtgctgtcgc cgaaggtgtt gccggtgttg
     2581 aacaccccga cgaccgtctt gtcggagccg ccgatgtgga gggtgtcgcc cggcccgacg
     2641 ccgatgtcct gggccaaccg gaccccgacc atgatctcgt ccgcggcctc ggcggtgaac
     2701 tcccgtcccg ccgcgagtcg caccccgaac ggagccaggt cctcggggcg gatccccacc
     2761 tcgaccacga gcgggtggtc cttgtcgagc gcctcggtgt ccagcagcac cccgatggcg
     2821 ctcttcaccc cgggggtctt ctgcacacgg gtcagctgct gctcggtcag ggcgctttcg
     2881 aggatgtcgc tgaggccctt ctgcgagatc gtgaagtcgg ccgcgccaac cttcagcacc
     2941 cccgcggcgg tggtgcggat gctgtcggtg acgatcccga gggccacgac gacgagcacc
     3001 ccgacggcca ccgcggcggc ggtcagcatg gaccgcacct tcttggacca cacgttgtgg
     3061 ccgatcagcc agatgaacga catgggcgac tcctcgtcgc gttcgcgttc gggagctcgg
     3121 gccacggcgc cgtccgccgg gccgtcaccc agtgtgccgc gggtgaacgc tccgtgaccg
     3181 gcatggtcgc cgactcgcta cggtgcgccc atggcggagg cccgacccga cgacgtgatc
     3241 cggatcgacg ccggagggtg gggactgagt tgggcaccct cggccgacgg caccctgcac
     3301 caggtcgggc tcggtcccgc cggggccgcc gcgacgcgcg acgtcgatgc ccactggtac
     3361 ccgagggcgt tcccgacgct cgacggcacc gaccccttcc gcccgccggc cctgcacgtc
     3421 gtccacgcgg acggaaccct gagcacgcgg ctcgcgctcg acgacgtcga ccgttccgac
     3481 gcggcggacg gctccggcga gcacgtcgtg ctcacgacgg tcgacgagct gttcgacctc
     3541 acggtggagc accacctgcg cacccatccc gactccggcg tgctcgagca gtgggtggag
     3601 gtcgtcaacg ccgaggacgg gccggtgacc ctgctctccc acgactcgat cgcgccgttc
     3661 ctgatggtcc ccgacgacgc cgaatacgtg cagttcggtg gggcgggctg ggccgacgag
     3721 tggcgttgga gcgccgggcg accggtgatc ggcaccacgg tcctcggcag cctcggtggg
     3781 gtccagcccc acctgcaacg cagcccgtgc ctgctcctgt cgcccaccgg tccgttcgac
     3841 gtcgacgccg cgggtgcacc ggcccaggac cgaggtgagg tgatcggcct cagcgtggcc
     3901 tggggaggca actcgcacgt ctcgctcgac ctgcgccccc gcgccgaacc cggcgcgccc
     3961 ggcgagctgc gcgtgcgggc cggcgcccac gccctcggag cgccgtaccg actcgacccc
     4021 gggacgcgga tggtgctgcc gacggtggcc tgggtgtggt cgagcgccgg cagggacggc
     4081 gtgaccgagg cgttccaccg ctggagccgg gcccgggtgc tgcgggatcc ggaccgccgc
     4141 cgcccgctgg tggtcaacaa ctgggaggcg acctacttcg acttcgacga gcaccgcatc
     4201 gtcgggctca tcgaccgggc cgccgagctc ggagccgacg tgttcctgct cgacgacggc
     4261 tggttcggca cgacccgacc ccgcgacgac gacacccagg gcctcggtga ctgggacgtc
     4321 gaccggcgca agctgcccgg tgggctcggc ccgctgaccg acgcggcggc cgagcggggg
     4381 atccgcttcg ggatctgggt cgagcccgag atggtcaacc cgctcagcga cctctacgac
     4441 gaccatccgg agtgggtcgc ccgcgacgtc cgcgaacccc gggagcaccg ccagcagctc
     4501 tacctggacc cactccgacc cgaggtgcgg gacttcgagg tcgacgtcgt cggccagacc
     4561 ctgcgatccg ccccggggac cagctacgtc aagtgggacg ccaaccgacc cgtcaccgac
     4621 cccggctccg ccacgcttcc ggccgatcga caggccaacg tctggttcga ccaggtcggc
     4681 gccacctggg acgtgatggc ccgcgtcgcc gccgcccacc ccgacgtgga gctcatgttg
     4741 tgcgcgtcgg ggggtggccg ggtggaccac gggtcgttgc ggtggttcca cgagttctgg
     4801 acctccgaca acaccgaccc cgtcgcccgg gtccgcatgc agtgggcgtg ctcgcacttc
     4861 ttccccgggg cggtgatggc cgcgcacgtg acacgttggg gggagcgccc gatggcgttc
     4921 gcgagcgcgg tggctctgtc gggccgcttc ggcatcgacc tggacctcga aacgaccacc
     4981 ccgtccgagg acgacgtcct gagccgggcc gtcgagtggg cgcgacgcac ccaggacgtg
     5041 gtgcagcagg ggacggtgcg gcgctacgtc tcgccggtcg acggagcgga ccgctcccga
     5101 gctgcgttct cggtcacgct gggggaccgc accgtcgtgt tcgcctacca gttggaggag
     5161 tcgacctcga ccccgccggt gatccgactc cgaggtctgc cccgggcggg cacctacagg
     5221 gtccgtacga ccgacctgca cggacccgct gaggagacga cctccaccga cgacgagttg
     5281 gaccgcggga tcgaatggcc gctcgaccga ccgctgtcgg cgcgcctgtg ggagatcgaa
     5341 cccgcccgcc ggacggcgtg agcgaccgca ccggagcgag cgtcgatcac gcggcggtgg
     5401 ggcggcgggg gacgtcggcc ccctgcctcc tcaacgccgc gacgcacacc catccgatcg
     5461 gcacgaccag ccagtgcgac atcaggcgga aggcgaccga cgccgccagc gccgttgacg
     5521 cgtccgtccc ggtggcggtg agaccgacca ccagcgccgc ctccacctga ccggccccac
     5581 cggggatgat cgggatcgct cccgccagct gagccgttcc gtacaccgcc agcagccacc
     5641 cccaggtcgc cgggtccgac gggatcgatc cgcccgtcgc ggcgaccgcc gcgaccagcg
     5701 acacgaactc gccgaaccag ctgagtacct gcagagccca cgccgcgctc caccgtccga
     5761 gcccggcgtg gaccgcaccg aaggacgcga cgaagcggtc ggcggcccgg tcggcctggt
     5821 cgtggaggtc gtggaggtcg tggaggtcgt ggaggtcgtg gaggctcccg ggctcccggg
     5881 cgggcctgcc cctgatccgg cgccggatgc ccgaccacga cccgacggcg atccgggcgg
     5941 gcgcccggag aagtccgggg cgccgggcga caacgaagaa cgccaccagc agcgtgcccg
     6001 cgacgagcgt gggggcgacg gcgaccgacg ggtcctcgcc ggccagggcg tagccggtca
     6061 gggtcatgaa cacaagaacc gtcggcgcga cgaccccggt ggcgatcgtg gcccacgccg
     6121 ctgcgccgcc gcggcgccgg cctcgcggta gcgctggacc atgtagccgg tggcgatcgc
     6181 tccgccggcg gggagcgtgg tggcgatcgc cccgctggcg aagctggcgg tggtggcccg
     6241 accgagtggg aaccgtgccc cggaggctcc gaccaggacc gatcgcaccg cggcgaacac
     6301 gacgatggag aggatcgagc tgaccgagcc gacggcgatc cagaccgggt ctgcgccgac
     6361 cagcaggtcg acagcggcca ggacctggtc gcgctccacg acgaggaacc agacgaggcc
     6421 ggccgcggcg agcacggcca cgccgacgcg gagtccccgg cggcgaccct gggtgcggac
     6481 cgagccggcg gtgccgtcgc cgccctccat ggccgaatgg taggagcagg gcccggaccg
     6541 gccccgtcaa gccttcgggt ggggtcggtt ggctagggtt cacccatgac cgccacccag
     6601 cgaccgagga ccccgcgctc gtcggctccg ccgcccggtc ccaacccggt gaacagaggg
     6661 ctcatcctca tcgcggtcgg ggtggtcctg gccgtcatcc tgctggtcaa ggccggcggc
     6721 gtcggcttcg actcctcctc gaccgacgtc gccatcggcg agggcgagga gaaggtcacc
     6781 accaccaccg aagcgaccac caccaccgtc gccgagcagg ctccgcagac cgtccaggtc
     6841 gtcgccgcca acggctcggg ggcgagcggc atggccgcca aggccggcca ggtgctcgcc
     6901 cagaacggct acacacaggt ggtcaccacc aacgccctcc agccggtcac ggcctcgtcg
     6961 gtgctctacg ccaccggcta cgacgccaac gcccggtcga tcgccaagat gttcggcctg
     7021 gccgagtcgg tcgtccagcc gctggcggcg gggacccaac tcgccaagga gcagccgccc
     7081 accaccggcg tcgtcgtcat gatcggaccg gacctgaccc agaccctcag cggcgtgggc
     7141 gccacggcca ccaccgtcgc cgggtcctcg ggaggagtca ccacgacggt caagtcgggc
     7201 agcggcggaa cgaccaacac cacggtcaag tcgggcacgg ctggggcgac caccaccacg
     7261 gtgaaggcga ccagcggtgc cggcgcggtg accaccacgg tcaaggccgc cacgacgact
     7321 cgctgagcga ccgtcgtgga tcggtcctcg ctgctcgagc cgttccacac ccacgccgcg
     7381 gacgccctgg tggcgttcga cttcgacggc acgttggcgc ccatcgtcga ggatcccgac
     7441 gcggcgtcgc cgatggccgg ggcggtgtcc gccctcgagc ggctcgcggc tcgcgtcggg
     7501 gaggtggccg tcgtgtccgg ccggccgctc gacttcctgg agcgatggtt cccggagccg
     7561 tccgcggtga cgctggtcgg cctgtacggg ttggagagtc gccgccaggg cgaccgggcc
     7621 gatcacccca gcgccggcgt ctggcgcgag gtcgtgtcgg acctggccga acgggcccga
     7681 cgtcacggcc ccgacgggat ggacgtcgaa ccgaaggggc tgtcgatcac gctgcactac
     7741 cgccgccgtc ccgacgtgga gccgtcggtg cgcgactggg cggaacgagc cgcgggagcc
     7801 acgggcctgt cggtgaggtc ggccaagatg agcgtcgagc tgcacccgcc gatcgacgag
     7861 gacaagggca cggtcctgca ccgcctcgcc gggttccaca ccgggcccgt cctcttcgcc
     7921 ggcgacgacg tcggcgacgt gccggcgttc gacgccctcg gcgggctggc cgccgccggt
     7981 cgcccggtgc tggccatcgt ggtcgacggc cccgaggtcc cgtcgacgct gcgccggcgg
     8041 gcggacctgt tggtcgacgg acccgccgag gtggccgaca tcgtcctctc cctcgccggc
     8101 tgaggtccga tcaccggggg ctctcccggt tcaggtcgtc ccggccgcgc ggagctgatc
     8161 ctgcagccag tcgcgcgggg tgcgcgcc
//
LOCUS       DAOQOD010000135         8157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3264_length_8157_cov_10.510244, whole genome shotgun sequence.
ACCESSION   DAOQOD010000135 DAOQOD010000000
VERSION     DAOQOD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_8157_cov_10.510244"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLZ72_12065"
     CDS             <1..252
                     /locus_tag="PLZ72_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37079.1"
                     /translation="EPPSTYYYRQIYGCFFDDAYGLKNIDQVGVDNACFETDYPHSDS
                     TWPHTKEVALKQMGHLPLELTRKLVRGNAIALLNLDLEP"
     gene            254..1714
                     /locus_tag="PLZ72_12070"
     CDS             254..1714
                     /locus_tag="PLZ72_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA37080.1"
                     /translation="MSDSTYRLTIGGESVEGAAGTYDIVNPATEELAGRAPEASVEQA
                     NAAVEAAAAAFPAWSRTAPAERAALLNRAADLIEEHRKEIVPVLQAETGATARTVKSM
                     QFFPATARLRRYATGAMQSNIVPLPPAVMPTTPLAPGGLISAAVHRAPVGVVTCITSY
                     NVPMTNMAGKIGPALAMGNTVIVKPAPQDPLGVLRMVELMNEAGFPPGVINVVTGQDI
                     APSEAVVAHPATDMVSFTGSTAVGQNIGAVCGSQMKRQLMELGGKGACIVFDDADLKA
                     AIGGISSVWGFHSGQICTSPTRVIVQRGIHDHLVGGLLKAASHMRIGDPTDPETVVGP
                     VISAAHRERIEGYVRAGVDEGATVAVGGGRSDMERGFYLEPTLLVDCTNQMTPVREEL
                     FGPVVVVVPFDDEDEAVAIANDSDYGLYSYVFSADTARAWGVAQRLRSGNVGINTLQR
                     NHEAPFGGFKRSGVGRDGGDFGLHAYSEMQSIVWPS"
     gene            1814..2515
                     /locus_tag="PLZ72_12075"
     CDS             1814..2515
                     /locus_tag="PLZ72_12075"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNA37081.1"
                     /translation="MVDLADELELSDPQTRVLGALLEKKATTPDAYPLTLKALVTACN
                     QSTSRDPVVDYDANLVETTCEALKAKGLLRILHPAAGERATKYRQILDEQLHLDDAEA
                     AVLAVLLLRGAQTAPELRSRTERMHAFGDVGSVEATLGRLAARSDRPLVRLLERAPGQ
                     REARWIQLLQHDADGRAEASASAGAGGGDRGSASPRGAAGAARIDDLEARVVELEAQV
                     ARLVAALDGLIDPPS"
     gene            complement(2520..3263)
                     /locus_tag="PLZ72_12080"
     CDS             complement(2520..3263)
                     /locus_tag="PLZ72_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37082.1"
                     /translation="MRDPVPAPEVPRLGYRVLERRGPQDRLLVLCHGYGLPVSDLTDR
                     LGLLDPDGRFLVVTPTAPFRHRDQVIWHKPTMSTDAAAQYMASLAALDDLLGHLGTTT
                     GRAAADAVVGGFSQGGSLALSLLLHADVVDRPAAAFGIHSFPAHVEGFRVDRAAAAGR
                     PAFLSGARNDRFAPVDMYRAGASLFRSVGLALTYREHDGDHDMSDEAAVELGEWLASL
                     GEPGPATEPDPDASGWSGPSPFQDLWKIL"
     gene            3344..4432
                     /locus_tag="PLZ72_12085"
     CDS             3344..4432
                     /locus_tag="PLZ72_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNA37083.1"
                     /translation="MRAIVWDGTAASVESDIQVRDLRAGEVRVRIEAAGLCHSDVSVL
                     NGTIMFPPPVVLGHEGAGEVLAVAPDVTNVAVGDHVVLSTLGNCGQCASCDSGKPTFC
                     RQSLGKLSRPFTKGDPDDPQKVFQFANLGVFCEETVVKATQAVKIPDDVPLTSASLIG
                     CGVLTGVGAVFNRAKVAHGQSVVVIGVGGIGLNAIQGAALSDALPVIAVDTNPAKEAY
                     AKLFGATHFICPDGPDFGLVEAVKEIQPNGVDFVFECVGSTALIRTSTDLLDWGGTLV
                     MLGVPKMGSEASFVVSTMYNDKTIMGCRYGSARPQYDIPLMVRLYQAGRLKLDELVSQ
                     TYPLEDFQLALDELHEGKLARGVLNVTG"
     gene            4445..5401
                     /locus_tag="PLZ72_12090"
     CDS             4445..5401
                     /locus_tag="PLZ72_12090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNA37084.1"
                     /translation="MPLPDEIATLAAEVSNWGRWGDDDELGCGNLLSAESTRRGTAAV
                     RDGRRISLAAELKADGIQVGQPARRYNPILTVTSLNERDKFAPGIWEGTDDLVTMSTC
                     AGTHVDALSHVSYDGFLYNGVPTSAVTAQYGASKLGAEKLPPIVTRGLLLDVARAKGV
                     DSLDEISPGYAITAEDLDAAAELVRVTPAPGDVILVRTGQMRHYHAGDRQRYTVGTDF
                     SQPGLSVHTIGWIHRHDLAGAFVDTYAYEAFPPTQADWSDCLAVHLLQIRDMGLLQGQ
                     NWDLEELADACAERGSGEVLLVAAPEPLVGATSAPVAPVAVL"
     gene            5424..6116
                     /locus_tag="PLZ72_12095"
     CDS             5424..6116
                     /locus_tag="PLZ72_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37085.1"
                     /translation="MTDPSVPGPPGDQRRSPWDPPAGPLPSAPVPPVPSGEGEAPPWG
                     SWTPPPATPGGPFPSAGGPSGASAGGSEPLRGVKGPRYGSPRFSGRGILAVFGLLGVL
                     VTLGIMVLLVVRVLDGVGGSSAGDASDLDGLVTTVPAPSGPSATTASGPAGVSPAGPV
                     GAAEAGACAADRETVSTAVEAYHVMNGSYPPDVQSLLDAGLVSFDGPVDMELHINGDT
                     VVVVGTGPCAGR"
     gene            6124..>6467
                     /locus_tag="PLZ72_12100"
     CDS             6124..>6467
                     /locus_tag="PLZ72_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37086.1"
                     /translation="MTERERTYSIWPFLAVFLVLGAVGLAGILIVGNPTTPTETIPAF
                     TTTSTTTPPSTAPEPAVTVPGGAPAPDGVKGVVVADGTTTYNFRRPAELSAGPLRAAA
                     ARSLVTAGIDGSS"
     assembly_gap    6468..6567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6615..8150
                     /locus_tag="PLZ72_12105"
     CDS             6615..8150
                     /locus_tag="PLZ72_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA37087.1"
                     /translation="MVEGRTLWELMERRAEATPDALMAVDEDMRTLTFAELWSESERT
                     AAGLQASGLRAGDVVTWQLPTWIESLILVAALSRLEVVQNPVLPILRDREVDFITDQA
                     DSRLLVVPSRWKGFDHEAMALAIAERQGRDMRVLVSDRALPQGDPTRLPDRAEPLDMA
                     DQPVRWIFYTSGTTAEPKGAQHTDASIAAVARGMGRRLAVIEDDRAALVFPFTHIGGV
                     TWLFTSLMFGNALILTDTFDPDGTPEVLSREGVTLAGAGTVFHQAYLAYQRKHLRPVF
                     PHVRAFPGGGAPKPPSLVEELRVLFDAPVLAGYGLTEAPILTMADVSDRDDELAVTEG
                     KPMTGVELRIHRLDGSPAAPGEEGEIRARAPQMMRGYLDPLLDIEAFDADGFFRTGDL
                     GRLDDRGNLIITGRLKDVIIRKGENVSAQEVEDLLITLPAVADVAVIGVPDPDAGERV
                     CAVVALADPDGTLELPEVVDHLRAQGLMAQKLPEQLEVVPAIPRNATGKVLKHVLRDE
                     FKG"
BASE COUNT         1160 a         2990 c         2816 g         1091 t
ORIGIN      
        1 gagccgccga gcacctacta ctaccgccag atctacggct gcttcttcga cgacgcctac
       61 ggcctgaaga acatcgacca ggtcggcgtc gacaacgcct gcttcgagac cgactacccg
      121 cactcggact cgacctggcc ccacaccaag gaggtcgccc tcaagcagat gggccacctc
      181 ccgctggagc tcacccgcaa gctggtgcgc ggcaacgcca tcgcgctgct caacctcgac
      241 ctggagccct gagttgtccg attccaccta ccgcctgacc atcggcggtg agtcggtcga
      301 aggggccgcc ggcacctacg acatcgtcaa cccggccacc gaggagctcg ccggtcgggc
      361 tcccgaggcc tccgtcgagc aggcgaacgc ggcggtcgag gccgcggcgg ccgccttccc
      421 ggcgtggtcg cggaccgcgc cggcagagcg cgccgcgctg ctcaaccggg ccgcggacct
      481 gatcgaggag caccgcaagg agatcgtccc ggtcctgcag gccgagaccg gggccacggc
      541 ccggacggtc aagtccatgc agttcttccc ggccacggcc cggttgcgcc gctacgcgac
      601 cggcgcgatg cagtccaaca tcgtgccgct gccgccggcg gtcatgccca ccacaccgct
      661 ggcgcccggc gggctgatct cggccgcggt gcaccgcgcc ccggtcggcg tggtgacctg
      721 catcacctcc tacaacgtcc cgatgaccaa catggccggc aagatcggcc ccgcgttggc
      781 catgggcaac acggtgatcg tcaagcccgc cccgcaggac ccgctcggcg tgctgcgcat
      841 ggtcgagctg atgaacgagg ccggcttccc gcccggggtg atcaacgtgg tcaccggtca
      901 ggacatcgcg ccgtcggagg ccgtcgtcgc ccaccccgcc accgacatgg tcagcttcac
      961 cggctcgacc gcggtcggcc agaacatcgg cgcggtgtgc ggctcgcaga tgaagcgtca
     1021 gctgatggag ctcggcggca agggcgcctg catcgtcttc gacgacgcgg atctgaaggc
     1081 tgcgatcggc ggcatcagct cggtgtgggg cttccactcc ggtcagatct gcacgtcgcc
     1141 cacccgggtg atcgtccagc gcgggatcca cgaccacctg gtcgggggcc tgctcaaggc
     1201 cgcgtcgcac atgcgcatcg gcgatccgac cgacccggag acggtcgtcg gaccggtcat
     1261 ctcggccgcg caccgcgagc gcatcgaggg ctatgtccgc gccggggtcg acgagggcgc
     1321 caccgtcgcc gtcggcggcg gccgcagcga catggagcgc ggcttctatc tggagccgac
     1381 gctgctggtc gactgcacca accagatgac gccggtgcgc gaggagctgt tcggaccggt
     1441 cgtcgtggtc gtccccttcg acgacgagga cgaggcggtc gccatcgcca acgacagcga
     1501 ctacggcctg tacagctacg tcttctccgc cgacacggcc cgcgcctggg gtgtggccca
     1561 gcggctgcgg tccggcaacg tcgggatcaa caccctccag cgcaaccacg aggcaccgtt
     1621 cgggggcttc aagcgctccg gtgtggggcg cgacggcggc gacttcgggc tgcacgccta
     1681 ctccgagatg cagtcgatcg tctggcccag ctgaccccac ccggagaatc ggggtcgtcg
     1741 tcggcacgtc ctgtcgattc cgcgccccgg aacgccccag gacgccgcgg ggtgggccgg
     1801 tactgtccgg gtcatggtcg acctggctga cgagctcgag ctgtccgatc cgcagacccg
     1861 cgtcctcggt gccctgctcg agaagaaggc gaccaccccc gacgcctatc cgctgacgct
     1921 caaggcgctg gtcaccgcct gcaaccagag caccagtcgg gacccggtgg tcgactacga
     1981 cgccaacctg gtcgagacga cgtgtgaggc gctcaaggcc aaggggctgc tgcggatcct
     2041 gcacccggcc gcgggggagc gggcgaccaa gtaccgccag atcctcgacg agcagctgca
     2101 cctcgacgac gccgaggcgg cggtgctcgc ggtgctgctg ctgcgcggcg cccagaccgc
     2161 acccgagctg cggagccgca ccgaacggat gcacgcgttc ggcgacgtcg gttcggtcga
     2221 ggcaaccctg ggccgcctcg cggcccgctc cgaccggccg ctggtgcggt tgctcgagcg
     2281 tgctccgggc cagcgcgaag cccggtggat ccaactgctg cagcacgacg ccgacggccg
     2341 cgccgaggca tccgcgtcgg cgggagccgg tggtggtgac cgcgggtcgg catcgccgcg
     2401 gggtgcggcc ggtgccgcac ggatcgacga cctcgaggcc cgggtggtcg agctcgaggc
     2461 ccaggtcgcc cggttggtcg ccgcgctcga cgggctgatc gacccgccgt cctgacggct
     2521 cagaggatct tccagagatc ctggaacggc gacggaccgg accaccccga cgcatccgga
     2581 tcgggctcgg tcgccgggcc cggctcgccc agggatgcca gccactcgcc gagttcgacc
     2641 gcggcctcgt cggacatgtc gtggtcgccg tcgtgctcgc ggtaggtgag cgccagcccc
     2701 accgaccgga acagcgaggc gccggcccgg tacatgtcga ccggggcgaa gcggtcgttc
     2761 cgggcccccg acaggaacgc cgggcgcccg gcggccgcgg cacgatccac ccggaagccc
     2821 tcgacgtggg cggggaagct gtggatcccg aacgccgcgg ccgggcggtc gaccacgtcg
     2881 gcgtgcagca gcaacgacag cgccaggctc ccgccctggc tgaaaccgcc gaccaccgcg
     2941 tcggctgcgg cccggccggt ggtggtcccg aggtggccga ggaggtcgtc gagcgcggcc
     3001 agcgacgcca tgtactgcgc cgcggcgtcg gtcgacatcg tcggcttgtg ccagatcacc
     3061 tggtcgcggt ggcggaacgg ggcggtgggg gtgacgacca ggaagcggcc gtcgggatcg
     3121 aggagcccca ggcgatcggt cagatccgac accggcagcc cgtagccgtg gcacaggacc
     3181 agcagccggt cctgcggccc gcggcgctcc agcacccggt acccgagccg ggggacctcc
     3241 ggtgccggga cggggtcgcg catcggcgca gtcgaccacg ccccggcgca gcgcaccaag
     3301 ctcgaacagg aacgcgttct acccaccggg tagggtccgg accatgagag cgatcgtgtg
     3361 ggacggcacc gccgcgtccg tcgagtccga catccaggtc cgggacctgc gcgccggtga
     3421 ggtccgcgtc cgcatcgagg ccgccggcct ctgccacagc gacgtgtcgg tcctgaacgg
     3481 cacgatcatg ttcccgcccc cggtcgtgct gggccacgag ggtgcgggcg aggtgctcgc
     3541 cgtcgccccc gacgtcacca acgtcgccgt cggcgaccac gtcgtgctgt cgacgctggg
     3601 caactgcggc cagtgcgcca gctgcgacag cggcaagccg accttctgcc gccagtcgct
     3661 cggcaagctg tcgcggccgt tcaccaaggg cgaccccgac gacccgcaga aggtgttcca
     3721 gttcgccaac ctcggcgtct tctgcgagga gacggtggtc aaggccaccc aggcggtgaa
     3781 gatccccgac gacgtgccgc tcacctcggc gtcgctcatc ggctgcgggg tgctgaccgg
     3841 cgtcggcgcc gtgttcaacc gggccaaggt cgcccacggc cagtcggtcg tcgtgatcgg
     3901 cgtcggcggc atcgggctca acgccatcca gggcgcggcg ctgtccgacg ccctgccggt
     3961 catcgcggtc gacaccaacc cggccaagga ggcctacgcc aagctgttcg gcgccaccca
     4021 cttcatctgc cccgacggcc ccgacttcgg cctggtcgag gctgtcaagg agatccagcc
     4081 gaacggcgtc gacttcgtgt tcgagtgcgt cggatcgacg gcgttgatcc gcacctcgac
     4141 cgacctgctc gactggggcg gcacgctggt catgctcggc gtgcccaaga tgggctccga
     4201 ggcgagcttc gtcgtgtcga ccatgtacaa cgacaagacg atcatgggct gccgctacgg
     4261 ctcggcccgc ccccagtacg acatcccgct gatggtgcgg ctctaccagg ccggccgact
     4321 gaagctcgac gagctggtgt cgcagaccta cccgctcgag gacttccagc tcgcgctcga
     4381 cgagctccac gagggcaagc tcgcccgcgg cgtcctgaac gtcaccggct gaggagggtc
     4441 gaccgtgccg ctacccgacg agatcgccac gctcgcggcc gaggtgtcca actggggacg
     4501 ctggggtgac gacgacgagc tggggtgcgg caacctgctc agcgccgagt cgacgcgccg
     4561 ggggaccgcc gcggtgcgcg acggccgccg gatctcgctc gccgcggagc tcaaggccga
     4621 cggcatccag gtcggccagc cggcgcggcg ctacaacccg atcctgaccg tcacctcgct
     4681 gaacgagcgc gacaagttcg cccccgggat ctgggagggc accgacgacc tggtcaccat
     4741 gtcgacctgc gcgggcaccc acgtcgatgc gctcagccac gtgtcctacg acgggttcct
     4801 ctacaacggc gtgcccacct cggcggtgac cgcccagtac ggcgcgtcga agttgggcgc
     4861 ggagaagctg ccaccgatcg tcacgcgggg cctgctgctc gacgtcgccc gcgccaaggg
     4921 cgtcgacagc ctcgacgaga tctcgccggg ctacgcgatc accgccgagg acctcgacgc
     4981 cgcggccgaa ctggtccggg tgacccccgc ccccggcgac gtcatcctgg tgcgcaccgg
     5041 tcagatgcgg cactaccacg ccggggaccg ccagcggtac accgtcggga ccgacttcag
     5101 ccagcccggc ctgtcggtgc acacgatcgg ttggatccac cgccatgacc tggccggcgc
     5161 gttcgtcgac acgtacgcgt acgaggcgtt ccccccgacc caggccgact ggtccgactg
     5221 cctggcggtg cacctgctgc agatccgcga catggggctc ctgcagggcc agaactggga
     5281 cctcgaggag ctcgcggacg cctgtgccga gaggggatcc ggtgaggtgc tgctggtcgc
     5341 cgcgcccgag ccgctcgtgg gagccacctc ggcgccggtg gccccggtcg cggttctctg
     5401 acgtctcgcc gtagcgtggg gtcgtgaccg acccgtccgt tccgggcccg cccggcgacc
     5461 agcgccgctc cccgtgggat ccgcccgcgg ggccgctccc gtccgcgccg gtgcccccgg
     5521 tgccgtcggg tgagggcgag gccccgccgt ggggatcgtg gaccccgcca ccggccactc
     5581 ccggtggacc gttcccatcc gccggcgggc cctcaggcgc gtcggcgggc ggctccgaac
     5641 cgctccgcgg agtgaagggc ccccgctacg gcagcccgcg gttctccggt cggggcatcc
     5701 tggcggtgtt cggcctgctc ggcgtcctgg tgaccctggg catcatggtg ctgctggtgg
     5761 tccgcgtcct cgacggcgtc ggcggctcct cggccggcga cgcgtcggac ctcgacggcc
     5821 tcgtcaccac cgttcccgcc ccgtccgggc cctcggcgac gacggcgtcc gggccggccg
     5881 gggtgtcacc cgcggggccc gtcggcgccg cggaggccgg ggcctgcgcc gcggaccgcg
     5941 agaccgtcag cacggcggtc gaggcgtatc acgtcatgaa cggctcgtac ccccccgatg
     6001 tccagtccct gctcgacgcc ggcctggtca gcttcgacgg gcccgtcgac atggagctgc
     6061 acatcaacgg cgacacggtg gtggtggtcg gcaccggccc ctgcgccggc cggtgagcga
     6121 gctgtgaccg aacgggagcg cacctactcc atctggccgt tcctggccgt gttcctcgtc
     6181 ctgggcgcgg tcggcctggc cgggatactc atcgtcggca accccaccac gcccaccgag
     6241 accatcccgg cgttcaccac caccagcacc acgacgccgc cgagcaccgc gccggaaccc
     6301 gcggtgaccg tccccggtgg ggcaccggct cccgacgggg tcaagggcgt ggtggtcgcc
     6361 gacgggacca cgacgtacaa cttccggcgc ccggccgaac tgagcgcggg cccgctgcgg
     6421 gccgcggccg cccgcagcct ggtcaccgcc gggatcgacg ggtcgtcnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnatc ggaggacctc tccgagcccc gggcgcaggt
     6601 gacaggagac ggacgtggtc gaaggtcgga cgctctggga gctgatggag cgacgcgccg
     6661 aggccacccc cgacgccctg atggccgtcg acgaggacat gcgtacgctg accttcgccg
     6721 agctgtggtc cgaatcggag cggaccgcgg cggggctcca ggcctcgggt ctgcgagccg
     6781 gagacgtcgt cacctggcag ctgccgacct ggatcgagtc gctgatcctg gtcgcggcgc
     6841 tcagccggct cgaggtcgtg cagaaccccg tcctgccgat cctgcgcgac cgggaggtcg
     6901 acttcatcac cgaccaggcc gacagccggc tgctggtggt cccgtcgcgc tggaagggct
     6961 tcgaccacga ggcgatggcc ctggcgatcg ccgaacgtca gggccgcgac atgcgggtcc
     7021 tcgtcagcga ccgggcgctg ccccagggcg accccacccg gctgccggat cgggccgaac
     7081 ccctcgacat ggccgaccag ccggtgcgct ggatcttcta cacgtcgggc acgaccgccg
     7141 agccgaaggg cgcccagcac accgacgcgt cgatcgccgc ggtggcccgg ggcatgggtc
     7201 gacgcctggc ggtcatcgag gacgaccgtg ccgcgctggt gttccccttc acccacatcg
     7261 gcggcgtcac ctggctgttc accagcctga tgttcggcaa cgcgctgatc ctgaccgaca
     7321 ccttcgaccc cgacggcacc cccgaggtcc tgtcgcgcga gggcgtcacc ctggccgggg
     7381 cgggcaccgt gttccaccag gcgtacctgg cctaccaacg caagcacctc cgacctgtgt
     7441 tcccccacgt gcgggccttc cccgggggcg gcgcgcccaa gccgccgtcg ttggtcgaag
     7501 agctgcgcgt cctgttcgac gctcccgtgc tggccggcta cgggctgacc gaggccccca
     7561 tcctgaccat ggccgacgtg tcggaccgcg acgacgagct cgcggtcacc gagggcaagc
     7621 cgatgaccgg cgtcgagctg cgcatccacc gcctcgacgg ctcaccggcc gcgccggggg
     7681 aggagggcga gatccgcgcc cgtgctccgc agatgatgcg cggatacctc gaccccctgc
     7741 tcgacatcga ggccttcgac gccgacggct tcttccgcac cggcgacctg ggccgactcg
     7801 acgaccgggg gaacctgatc atcaccgggc ggctcaagga cgtgatcatc cgcaaggggg
     7861 agaacgtctc ggcccaggag gtcgaggacc tgctgatcac cctgccggcg gtggccgacg
     7921 tggcggtcat cggcgtgccc gaccccgacg ccggcgaacg ggtgtgcgcg gtggtcgccc
     7981 tggccgatcc tgacgggacc ctcgaactac ccgaggtggt cgaccacctc cgggctcagg
     8041 gcctgatggc ccagaagctc cccgagcagc tcgaggtcgt gcccgcgatc ccgcgcaacg
     8101 ccaccggcaa ggtgctcaag cacgtgctgc gcgacgagtt caagggctga gaccggg
//
LOCUS       DAOQOD010000136         8099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3293_length_8099_cov_13.623073, whole genome shotgun sequence.
ACCESSION   DAOQOD010000136 DAOQOD010000000
VERSION     DAOQOD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_8099_cov_13.623073"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /gene="dnaX"
                     /locus_tag="PLZ72_12110"
     CDS             complement(<1..1093)
                     /gene="dnaX"
                     /locus_tag="PLZ72_12110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNA37088.1"
                     /translation="MAHQSLYRRYRPRRFVEVRGQEHVVRALRNSVRTGTEGHAYLFS
                     GPRGTGKTSTARILAKALNCEDLRDGEPCCECSSCVEMEAGRSFDLFELDAASNNGVD
                     SIRDLVERAAVASPGRTKMYILDEVHMLTAGASNALLKTLEEPPEHVCFVLATTDPQK
                     VLPTIRSRTQHYDFQLLSAGELTDYVRWIAADAPLDVDDESVAYVVREGHGSARDTLS
                     ALDQVVAAGGVITRDLPVDQLLGAIAAGDSGAAVLAVSDALALGHDPRVLAESFLAEL
                     RDAFLLSLGVEVTHLVDADAERLGEWARTLGTPVLTRAVEAVGSALVDMRQAADPRVP
                     LDVAMVRLCVGAPTAGGAAGPAPAGRGSGD"
     gene            complement(1182..1269)
                     /locus_tag="PLZ72_12115"
     tRNA            complement(1182..1269)
                     /locus_tag="PLZ72_12115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1233..1235),aa:Ser,seq:gga)
     gene            1315..1413
                     /gene="ffs"
                     /locus_tag="PLZ72_12120"
     ncRNA           1315..1413
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLZ72_12120"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1678..1767)
                     /locus_tag="PLZ72_12125"
     tRNA            complement(1678..1767)
                     /locus_tag="PLZ72_12125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1731..1733),aa:Ser,seq:cga)
     gene            complement(1823..2326)
                     /locus_tag="PLZ72_12130"
     CDS             complement(1823..2326)
                     /locus_tag="PLZ72_12130"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNA37089.1"
                     /translation="MADRSPAPPVLEPDPEHAGWMAIALDEARAATLHGDVPIGAVVV
                     QLSEGAAPRVLTRRHNERELSHDGTAHAEVLALRDACAELGRWRLDDCLMVVTLEPCP
                     MCAGALWATRIGGVVIGTESMDAGSLGSLYHLGQDPRLNHEFPVRVGVERGACAAILQ
                     DFFAARR"
     gene            2589..3233
                     /locus_tag="PLZ72_12135"
     CDS             2589..3233
                     /locus_tag="PLZ72_12135"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA37090.1"
                     /translation="MKPGSDERGATDADDTDRPGLRERKKERVREQIRQAALDLFDQR
                     DFDSVTVDEIAERAEVSKSTLFRYFETKEDLVFANSRAYGDTLLAAFAERPSDESVLR
                     SLRAALVSLVSGLQADRSRYLRGQRIVARSPSLVRRSIERQYEWEDGLATALLPRLSN
                     HDDAETRASVLAAAGLAVVRIATRRWVTAADDSLLIDHLLPALDVLADELKDSD"
     gene            3236..4969
                     /locus_tag="PLZ72_12140"
     CDS             3236..4969
                     /locus_tag="PLZ72_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37091.1"
                     /translation="MLTEMLRVHLRPYRRPLLLVLALTTVQVACVLFLPTLNADIIDK
                     GVIPGDTDYIWRVGGIMLAVTFVQVLFAVASVYVGSRVAMAFGRDVRGALFHKVTGFA
                     AQDVAHFGAPSLITRITNDVTQVQMLVLMSCTLLVAAPITIVGGVIMAVREDGTLSLL
                     LLVSVPVLVISIGSIIVRMIPLFQVMQERIDRINEVLREQITGMRVVRAFVREDDEGA
                     RFEEANDALTLVSLRSGRLMAFMFPIVMAVLNLSSVAAIWLGADRVADGELQIGALIA
                     FLSYLIQILMSVMMATFVAVLTPRASVSANRIGEVLHTESAVAQAVNPVTSLPERPVL
                     RFDHVEFRYPGAEHPVLCDQSFEVRAGTTLAIIGSTGSGKTTLLDLIPRLYDATAGTV
                     SVGGHDVRDIDQETLWSRIGLVPQKPFLFSGTVASNLRHSKPDATEDEMWEALRIAQA
                     EDFVRAMPDGLDSPIAQGGSNVSGGQRQRLAIARALVRRPDIYLFDDSFSALDLATDA
                     RLRAALVPVVTDSVTVLVAQRVSTIRHADQILVLEDGATVGLGTHDELLGSSPTYAEI
                     VASQLTAEEAA"
     gene            4966..6930
                     /locus_tag="PLZ72_12145"
     CDS             4966..6930
                     /locus_tag="PLZ72_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37092.1"
                     /translation="MSTSNDKPPTSGAGTTGDAADTTPDGSAGTDYAQSAKVNEGARG
                     AMGRFGAAGMPLDRSKDLGGSVRRLAGQMRPESVGLIGVVALAVISVTLLVLGPRILG
                     NATQTIVDGVVGQFKGGPGIDFSQLHRLLLTAAAIYVVATTLAYAQGWLLAGVVQRSM
                     FRLRADVETKLNRMPLSHVDRQPRGDLLSRVTNDIDNLAQSMQQTLSQLLSSSLQLVG
                     VLVMMFTISWTLALVTLIIVPVTILTMRAITSRSKKRFVDQWRHTGTLNAQVEEAFTG
                     HALVKVYNRQADTEERFRQKNEELFEASAAAQFMSGSIQPAVMFLGNVNYVAIALLGG
                     LLVTNGSMNIGDIQAFIQYSRQFTQPLTQVASMLNVMQSGIASAERVFELLDAPEEPE
                     SATTDLSSVDVRGRIEFRDVDFAYSPDKPLIQDLSLVAEPGETVAIVGPTGAGKTTMV
                     NLIMRFYDVDSGAILLDGIDTATISRQDLRRHMGMVLQDTWLFGGTIRDNIAYGNLNA
                     TEEQIMEAARATYVDRFVHSLPDGYDTVIDDEGGTVSAGEKQLLTIARAFLADPTILI
                     LDEATSSVDTRTEVLIQRAMAALRSNRTSFVIAHRLSTIRDADTILVMEHGHIVEQGN
                     HETLLAADGAYARLYNAQFAGAAVDVDVAS"
     gene            complement(7045..8043)
                     /locus_tag="PLZ72_12150"
     CDS             complement(7045..8043)
                     /locus_tag="PLZ72_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNA37093.1"
                     /translation="MLERPESFAVVRRRVLSAVAVIAAVVVVVARPADAAALVRAAAD
                     GTEELLSWWQAVILGVVEGVTEYLPISSTGHLLVTSRLLGLPSEEGSAGLEAVNTYVI
                     AIQFGAILAVVGLFWRRFRSMILGLFGRDDDGRQLLVVLVIAFLPSALVGFLFDGMIE
                     DALFGPWPVVIAWVIGGIVILVLERSGRIPDRGEKAEPGTDPLIDITVRQALIIGVVQ
                     CLALWPGTSRSLTTILAALLVGVAAPAAVEFSFLLGFATLSAASLYKVVADGGTLVEQ
                     FGVVTPLIGVLAAFVSAVLAIKWLVSYLERHDLTIFAWWRFGAAAITAGLLLAGTI"
BASE COUNT         1321 a         2899 c         2716 g         1163 t
ORIGIN      
        1 cgtcgcccga accgcgtccg gccggggccg gccccgcggc ccctccggcc gtcggagcgc
       61 cgacgcacag ccggaccatg gcgacgtcga gcggaacccg gggatccgcg gcttgacgca
      121 tgtcgaccag ggcggatccg accgcttcga ccgcccgggt caacaccggg gtcccgaggg
      181 tgcgggccca ctcgccgagt cgttcggcgt cggcgtcgac caggtgggtc acttcgactc
      241 cgagcgagag caggaacgcg tcgcggagct cggccaggaa cgactcggcc agcacccgcg
      301 ggtcgtggcc cagggccagg gcatcggaga cggccaggac cgcggctccg ctgtcgccgg
      361 ccgcgatcgc accgagcaac tggtcgacgg gcaggtcacg ggtgatcacc ccgccggcgg
      421 ccaccacctg gtcgagcgca gacagcgtgt cgcgggccga gccgtgaccc tcgcgcacga
      481 catacgcgac cgactcgtca tcgacgtcga gcggggcgtc ggccgcgatc cagcggacgt
      541 agtcggtgag ctcccccgcc gacaggagct ggaagtcgta gtgctgggtg cggctgcgga
      601 tggtgggcag caccttctgc gggtcggtgg tggccagcac gaagcacacg tgctccggtg
      661 gctcctcgag cgtcttcagc agcgcgttgg acgcccccgc cgtcagcatg tgcacctcgt
      721 cgaggatgta catcttggtg cggcccggcg acgcgaccgc ggcccgctcg acgaggtccc
      781 ggatggagtc cacgccgttg ttggacgcgg cgtcgagctc gaacaggtcg aagctgcgtc
      841 cggcctccat ctcgacgcac gagctgcact cgcagcaggg ttcgccgtcg cggaggtcct
      901 cgcagttgag cgccttggcc aggatccgag ccgtggaggt cttgccggtg ccacgagggc
      961 cggagaacag gtaggcgtgg ccctcggtgc cggtccgcac cgagttccgc agtgcccgga
     1021 cgacgtgctc ctgaccgcgg acctcgacga atcggcgggg tcggtagcga cggtagaggg
     1081 actggtgggc cacggcggga tcctaccgac gactccccga cggtcccggc cggagcaacg
     1141 gtgcggcctg cgcctccgga gtccggccgg gttccgcccg gtggcggaga cggtgggatt
     1201 cgaacccacg gtgagtttcc ccacacacgc tttccaagcg tgccgattcg gccgctctcg
     1261 cacgtctccg gggcgggacg atagcctgag tccgcgcctg cgtcctcagg cggcggccgg
     1321 gtcctgtgcg accggggccc gtgaatcggg tcagggccgg aaggcagcag ctctcagcgg
     1381 aacccccggt gtgccgcaga tcgcctggcc gccacctgag gacacaggcg ttcatcgcgc
     1441 caggcgccgg gccgaacttg tagctgacgg acgctcccac gacccgtggc ctacaagttc
     1501 atcgggccga accgaacttg tagctgacgg acgctcccac gacccgtggc ctacaagttc
     1561 atcgggccga accgaacttg tagctgacgg tcgcccccac gactcgtggc ctacaagttc
     1621 gtcgggccgc gtggagggcc ggaacgagct cgggcccctg cctgagcagg ggcccgatgg
     1681 cggagacggt gggattcgaa cccacggtgg ccttgcgacc acaacggttt tcgagaccgt
     1741 cccattcgtc cgctctggca cgtctccggc ggcgacgata gcggcgcagg gatcgtccgg
     1801 gcacaccggg atctggcccc gttcagcgac gggcggcgaa gaagtcctgg aggatcgccg
     1861 cacaggcgcc gcgctcgacg ccgacgcgga ccgggaactc gtggttgagc cgggggtcct
     1921 gtcccaggtg gtagaggctg ccgagagaac ctgcatccat gctctcggtt ccgatcacca
     1981 ccccgccgat gcgtgtggcc cacagcgccc ccgcgcacat cgggcacggc tcgagggtga
     2041 cgaccatcag acagtcgtcg agtcgccagc gacccagctc tgcgcacgcg tcacggaggg
     2101 cgaggacctc ggcgtgggcg gttccgtcgt ggctgagctc ccgctcgttg tggcgtcggg
     2161 tgagcacccg gggtgcggca ccctcggaga gctgcacgac gaccgctccg atggggacgt
     2221 cgccgtggag ggttgcggca cgggcctcgt cgagagcgat cgccatccac ccggcgtgct
     2281 ccggatcggg ttccagcacc ggaggggctg gggatcggtc ggccatcggc gccgacggta
     2341 ccgccgaacg cgcagcccac cgaacttgta gcccacgaac ccccacggcg accaccacct
     2401 acaacaacgc ggggcgccac caaacttgta gcccacgaac ccccacggcg accaccagct
     2461 acaacaacgc ggggcgccac cgaacttgta gcccacgtgc catccgagcg accgagggct
     2521 acaagttcgg cgagtcgcgg ttcaaccaga taaagtggca ctcggtgtca aacagtaccg
     2581 aggcacaaat gaagccgggt tccgacgagc ggggcgcgac cgacgccgac gacaccgatc
     2641 ggcccggtct gcgcgaacgc aagaaggagc gggtccgcga gcagatccga caggcagctc
     2701 tcgacctgtt cgaccagcgc gacttcgaca gcgtgaccgt cgacgagatc gccgagcgcg
     2761 ccgaggtctc caagagcacc ctcttccgct acttcgagac caaggaggac ctggtcttcg
     2821 ccaactcgcg cgcctacggc gacaccctcc tggccgcctt cgccgaacga ccctccgacg
     2881 aatcggtgtt gcgatccctg cgggccgcac tcgtgtccct cgtcagcggg ctccaggccg
     2941 accgctcgcg gtacctccgc ggccaacgca tcgtcgcccg gtccccgtcg ctggtccggc
     3001 ggtccatcga gcgtcagtac gagtgggagg acggactggc cacggcgctg ctcccccgcc
     3061 tgagcaacca cgacgacgcc gagacccgcg cctcggtcct ggccgcggcc ggcctggccg
     3121 tcgtccgcat cgcaacacgc cgctgggtga ccgccgcgga cgactcgctc ctgatcgacc
     3181 acctcctgcc tgcgctcgac gtgctggccg acgaactgaa ggactccgac tgacgatgct
     3241 gaccgagatg ctgcgggtcc acctgcggcc ctaccggcga ccgctcctgc tggtgctcgc
     3301 cctcaccacc gtgcaggtgg cgtgcgtcct gttcctgccg accctcaacg ccgacatcat
     3361 cgacaagggc gtcatcccgg gcgacaccga ctacatctgg cgcgtcggcg ggatcatgct
     3421 ggcggtcacc ttcgtccagg tcctgttcgc ggtggccagc gtctacgtgg gttcacgggt
     3481 ggcgatggcg ttcgggcgcg acgtccgcgg tgccctcttc cacaaggtga ccggcttcgc
     3541 cgcgcaggac gtggcgcact tcggagcccc gtcgctcatc acccgcatca ccaacgacgt
     3601 cacccaggtc cagatgctgg tcctgatgag ctgcacgctt ctggtcgcgg caccgatcac
     3661 gatcgtcggc ggagtgatca tggccgtccg cgaggacggc acactgtcgc tgctgctcct
     3721 ggtcagcgtc ccggtgctgg tgatctcgat cggttcgatc atcgtgcgga tgatccctct
     3781 gttccaggtc atgcaggagc gcatcgaccg catcaacgaa gtgctaagag agcagatcac
     3841 cggcatgcgg gtcgtccggg cgttcgtccg tgaggatgac gagggagccc gcttcgagga
     3901 ggccaacgac gcgctcaccc tggtgtcgct gcggtcgggc cgcctgatgg cgttcatgtt
     3961 cccgatcgtg atggccgtgc tcaacctgtc gagcgtggcc gccatctggc tcggggcgga
     4021 ccgggtggcc gacggagagc tgcagatcgg ggcgttgatc gcgttcctga gctacctgat
     4081 ccagatcctg atgtcggtga tgatggcgac cttcgtggcc gtcctcaccc cgcgggcctc
     4141 ggtgagcgcg aaccgcatcg gcgaggtgct gcacaccgaa tccgcggtcg cccaggcggt
     4201 gaacccggtg acatctctgc ccgagcgccc ggtgctgcgc ttcgaccacg tcgagttccg
     4261 ctaccccggc gcagagcacc cggtcctgtg cgaccagtcc ttcgaggtcc gcgccggaac
     4321 caccctggcg atcatcggct ccacgggatc gggcaagacc accctcctcg acctgatccc
     4381 ccggctctac gacgccaccg ccggaacggt gtcggtcggc ggccacgacg tgcgcgacat
     4441 cgaccaggag accctctgga gccggatcgg actcgtgccc cagaagccgt tcctcttctc
     4501 gggcacggtg gcgagcaacc tgcgccactc caaacccgac gccaccgagg acgagatgtg
     4561 ggaggcgctg cgcatcgcgc aggccgagga cttcgtgcgg gccatgcccg acggcctcga
     4621 ctcgcccatc gcacagggcg gctccaacgt gtcgggcggc cagcgccagc gcctggccat
     4681 cgcccgggca ctggtgcgcc ggcccgacat ctacctgttc gacgactcgt tctcggccct
     4741 ggacctggcc accgacgccc ggctgcgcgc cgcgctcgtc cccgtggtga ccgactcggt
     4801 gacggtcctg gtcgcccaac gggtgtcgac catccgccac gcggatcaga tcctggtcct
     4861 cgaggacggc gccaccgtcg gtctcgggac ccacgacgag ctcctcggat cgtctccgac
     4921 ctacgccgag atcgtggcgt cgcagctgac cgcagaggag gcggcgtgag cacctcgaac
     4981 gacaagcccc cgaccagtgg cgccggcacg actggcgacg ccgcggacac gacccccgac
     5041 gggtccgccg ggaccgacta cgcccagtcc gccaaggtca acgagggcgc ccggggcgcc
     5101 atggggcggt tcggcgcggc gggaatgccg ctcgaccgat ccaaggacct gggcgggtca
     5161 gtgcgacgcc tggccgggca gatgcgaccg gagagcgtcg gcctgatcgg cgtggtcgcc
     5221 ctggccgtca tcagcgtcac gctgctggtg ctcggtcccc gaatcctcgg caacgccacc
     5281 cagaccatcg tcgacggcgt cgtcggccag ttcaagggcg gtcccggcat cgacttcagt
     5341 cagctgcacc gactcctgct gaccgcggcg gcgatctacg tggtcgccac gaccctggcc
     5401 tacgcccagg gctggctgct cgccggcgtc gtgcagcgct cgatgttccg gctccgtgcc
     5461 gacgtcgaga cgaagctcaa ccggatgccg ctcagccacg tcgaccgcca gccccgcggc
     5521 gacctgctca gccgggtcac caacgacatc gacaacctgg cccagagcat gcagcagacg
     5581 ctgagccaac tgctgtcctc cagcctccag ctcgtcgggg tgctggtgat gatgttcacc
     5641 atctcgtgga ccctggcgct ggtcacgctg atcatcgtcc cggtcacgat cctgaccatg
     5701 cgggcgatca cgtcgcggtc caagaagcgc ttcgtcgacc agtggcgcca caccggaacg
     5761 ctcaacgccc aggtcgagga ggcgttcacc ggccacgccc tggtcaaggt gtacaaccgc
     5821 caggccgaca ccgaggagcg gttccgccag aagaacgaag agctgttcga ggccagcgcg
     5881 gccgcgcagt tcatgtcggg atcgatccag ccggcggtga tgttcctcgg caacgtcaac
     5941 tacgtcgcca tcgccctgct cggtggcctc ctggtcacca acgggtcgat gaacatcggc
     6001 gacatccagg cgttcatcca gtactcccgg cagttcaccc aacccctgac gcaggtggcg
     6061 tcgatgttga acgtgatgca gtccggcatc gcttcggccg aacgggtctt cgagctgctc
     6121 gacgcccccg aggagcccga gagcgcaacg accgacctgt cgtcggtgga tgtgaggggc
     6181 cgcatcgagt tccgcgacgt cgacttcgcc tactcgccgg acaagccgct gatccaggac
     6241 ctgtcgctcg tggccgagcc gggagagacg gtcgccatcg tgggtccgac cggtgccggc
     6301 aagacgacga tggtgaacct gatcatgcgg ttctacgacg tcgactcggg cgccatcctc
     6361 ctcgacggga tcgacaccgc gacgatcagc cgccaggacc tccgccgcca catgggcatg
     6421 gtgctccagg acacctggct gttcggcggg acgatccgcg acaacatcgc ctacggcaac
     6481 ctgaacgcca ccgaggaaca gatcatggag gcggcccgcg ccacctacgt cgaccggttc
     6541 gtgcactcgc tgcccgacgg ctacgacacc gtgatcgacg acgagggcgg cacggtctcc
     6601 gccggcgaga agcagctgct gaccatcgcc cgggcgttcc tggcggatcc gacgatcctc
     6661 atcctcgatg aggccaccag ctcggtcgac acccgcaccg aggtgctgat ccagcgggcg
     6721 atggcggcgc tgcgcagcaa ccggaccagc ttcgtgatcg cgcaccggct gtcgacgatc
     6781 cgcgacgccg acacgatcct ggtcatggag cacggtcaca tcgtcgagca ggggaaccac
     6841 gagacactgc tcgccgcgga cggcgcctac gcgcggctgt acaacgccca gttcgccgga
     6901 gcggcggtcg acgtcgacgt cgcgtcctga acgggctcac ccgcgccgga cgtcgcagac
     6961 gggcgccacc cctcggcgcg ttctcatgac atctgagtac cgccagtgtc gtgacttgtc
     7021 gtcagaaacc tgggtggggg tgggtcagat ggtgccggcc aggaggaggc cggcggtgat
     7081 cgccgcggcg ccgaagcgcc accaggcgaa gatcgtcagg tcgtggcgct cgaggtagga
     7141 gaccagccac ttgatcgcca gcaccgcgga cacgaacgcg gcgagaactc cgatcagcgg
     7201 ggtcacgacc ccgaactgct cgacgagggt gcccccgtcg gcgacgacct tgtagagcga
     7261 tgccgcactc agggtcgcga accccagcag gaagctgaac tcgaccgccg cgggggccgc
     7321 gacgccgacg agcagggcgg ccaggatcgt ggtcagcgac cggctggttc ccggccacag
     7381 cgccaggcac tggaccacgc cgatgatcag cgcctgacgg accgtgatgt cgatcagcgg
     7441 gtcggtgccc ggctcggcct tctcgccccg atcggggatc cgcccgctgc gctccagaac
     7501 caggatcacg atgccaccga tcacccaggc gatcaccacc ggccacggcc cgaacagagc
     7561 gtcctcgatc atcccgtcga acaggaaccc gacgagggcc gagggcagga aggcgatcac
     7621 cagcaccacg agcagctgcc ggccgtcgtc gtcgcgaccg aacaggccga ggatcatcga
     7681 gcggaaccgc cgccagaaca ggccgaccac cgccaggatc gccccgaact ggatggcgat
     7741 cacgtacgtg ttcaccgcct cgagccccgc gctgccctcc tcgctgggca ggccgagcaa
     7801 ccgcgaggtc acgagtaggt gaccggtcga ggagatcggc aggtactcgg tgacgccctc
     7861 gaccacgccg aggatcacgg cctgccacca cgacagcagc tcttcggtcc cgtccgccgc
     7921 ggcccgcacc agtgccgctg cgtccgccgg tcgggccacg acgaccacga ccgcggcgat
     7981 cacggcgacc gccgacagga cccgccggcg gacgacggcg aaggactccg ggcgttcgag
     8041 catcgggaca gcttggcacg ctggtgaagc gctcctgatg cgccgctgta ggttcctgc
//
LOCUS       DAOQOD010000137         8098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3294_length_8098_cov_9.425339, whole genome shotgun sequence.
ACCESSION   DAOQOD010000137 DAOQOD010000000
VERSION     DAOQOD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_8098_cov_9.425339"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1993
                     /locus_tag="PLZ72_12155"
     CDS             53..1993
                     /locus_tag="PLZ72_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNA37094.1"
                     /translation="MADPARTRRRLWGMVAAFGVLALLPLVKLVGVQFADASTLAAEG
                     LQQRQRTESIPAPRGSILDRNGSELAISVPRTTVAVNKVVLARAGISDDAMLREFGTR
                     LAQLLDVDPADVVDSLLDADADARWVRVVRYADVDVARKAARSLAEQDMDGVLLLDPT
                     SERVHPGGESGVRVIGTVGPDGPGPRAAVEAKYDDVLKGVPGEQVVERGAQGETITGG
                     DRVVTAPRPGADVQVTLDRTLQYETEQILTKGSENARAAGGIAIVGRPSTGELLAVAG
                     VERNEKTGAIELSSRPLAFSDAYQAGSVFKLVTTAAAYEAGVIDDHSTFSVGPRIKVY
                     DREFSDNEAHAVETMNVDQIVAHSSNVGTIQIAQRLGAEKLHAALVDFGFGRVSALDL
                     PAESTGLLPDVDKWTPPDTAAASIGSFQSTTVLQLWSAFNVIANQGIYVPPRLVQGTI
                     GADGRLDPASAAPTRRVVSASTAARVSRALQAVVQVGTGKQWSLPGYPVAAKTGTGRM
                     PSPEKVDAKDAYVWPDGTYHHVTTFAGYLPADRPQVSITVMLFDTAQGLTGSTSAGPV
                     FSDLSRLAIRELSIAPTTTDAGSADGAGPVRSAPAGSTAAATDRALLPGRNGGGSTSS
                     RSPSTTIAPSGRTSRPGSGQQR"
     gene            2004..3512
                     /locus_tag="PLZ72_12160"
     CDS             2004..3512
                     /locus_tag="PLZ72_12160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNA37095.1"
                     /translation="MDPHESDGNDGTQEGHSSGGAAAAVTVGELLAALDATHPGARLH
                     GDASASAVPLRSVTHDSRRVGPGTLFCCVVGEHADGHDFAAEAVAAGAVALLVQRDPD
                     PDGPLRSVPQIVVDDVRAAMATAAVEVYRHPAERLRTVGVTGTNGKTTVVSAVAHVLS
                     AAGRRVEAIGTLTGARTTPEAPDLQARLAELVSAGVTDVAMEVSSHALSLHRVDGVVF
                     DVAVFTNLGQDHLDFHGTPEAYFAAKAQLFEAGRSRRGIVDVDDVHGRLLMDSAPPGH
                     PMVAVSVADAEPVTSTVTGSSFRWRGRSVSCPLPGRHNVANAMLAAEACAALGVEEDS
                     IVAALADLPVVPGRFERVDAGQDFGVVVDYAHTPDALEAVLVAARELGPGRVLVVFGC
                     GGDRDATKRPRMGAVASEHADVVVLTTDNPRSEDPLRILEAVRSGCSGDPVVEPDRRA
                     AIAVALGSARPGDVVVIAGKGHEQGQEVAGVVTPFDDRLVAAELLASTEGPS"
     gene            3509..4540
                     /gene="mraY"
                     /locus_tag="PLZ72_12165"
     CDS             3509..4540
                     /gene="mraY"
                     /locus_tag="PLZ72_12165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNA37096.1"
                     /translation="MIRLLLATGIAALVTLVGTRLLIGALTRRHIGQPIHEDVPEGHT
                     VKAGTPTMGGVAIVAGGLCGYLASNLYDGVFTWTGLAAMGAIAGAGTVGFLDDWIKIR
                     NERNLGLNKRSKTLGLLLVAVGFALVILWRTNVHTTISFGRFDDLGWTLGKWVWVAWA
                     VLVIFASSNAVNLTDGLDGLAAGSAIFVFSAYVIIGYWIFRHPGMYQINVGLDLAVVA
                     AAMMGACAGFLWWNAAPAQIFMGDTGSLAIGTGLAALALSSSTVVLLPILGLLFVMET
                     VSVIVQVASFRTTGRRVFRMAPIHHHFELGGWPETTVIIRGWMLTGATTAVGLGLFYR
                     EFIRAGGLA"
     gene            4537..5931
                     /gene="murD"
                     /locus_tag="PLZ72_12170"
     CDS             4537..5931
                     /gene="murD"
                     /locus_tag="PLZ72_12170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNA37097.1"
                     /translation="MTSPGLAERSLLLHGLGLTNLAVARAAVAHGVAVVASDDSSPDS
                     ASTLADDLGIEVVLGPSADQLDALVAGVDAVVPAPGLPETHPLFAAAAAHDRPVLSEF
                     DLAGAWDDRPVVAITGTNGKTTVTTLVTAMFERSGVRAAAVGNLETPLVEAIARDDID
                     VFVVEASSFRLAHSRRFAPSVGVWLNFAPDHLDVHRTLDDYRRAKARIWRDQTAVDTA
                     VVVAEDPVVDADALARADAPDAPRFVRVGLDAVIDGRPVPFHERDGVLVGVAGPDASP
                     VDLVAVADLWRSLPHDRTNALAAAAAALAGGASIDGVRAALVGFRGLPHRVELVGTSG
                     GIRYYDDSKATAPHATLAAVSGFESVVLIAGGRNKGLDLGELRRGTDRIRAVVGIGEA
                     GPDVLDALAGRPGRPADSMAAAVAAAADMAEPGDVVLLSPGCASFDWYGSYAERGDDF
                     ASCVRARADFEEPT"
     gene            5928..7166
                     /gene="ftsW"
                     /locus_tag="PLZ72_12175"
     CDS             5928..7166
                     /gene="ftsW"
                     /locus_tag="PLZ72_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNA37098.1"
                     /translation="MTATRRGGAGSSTKRGRTTAAARSGRAAPPRRPEISVVRRGPDP
                     STMVVVTVALLCGLGVVMVYSASSVTAVRTLGHSWGIVMRQAVWLVLGVAAAYGASRV
                     PWRIWRDRVALPLMLVSVALLGYVALGVLAQKATGSALPFVISVNGATRWVGAGSMQF
                     QPSDLAKPALILWLARLLSEKGRDVRTWEGLRPVLIWSGLTCGLVVFGDDLGTTMLLG
                     AVTLAMVFMAGAPLPKLGAIAGVSIGLGGLAIVGLERFRVTRILAFLDPDKYRQGAGW
                     QLWQSQIGLASGGVFGTGPGLARSKWGFLPEAHTDFILAVIGEELGLVGSLIVLGLFV
                     VFMAAGCAIGMRARTGFTRLVAFGITTWIGVQALINIGVTVGTLPTKGITLPFVSYGG
                     SSLLMSLVGVGILLSVAREA"
     gene            7169..>8098
                     /locus_tag="PLZ72_12180"
     CDS             7169..>8098
                     /locus_tag="PLZ72_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNA37099.1"
                     /translation="MSAAPPTWAIVAGGGTAGHVLPGLAVARELVDRGHRPETLHFVG
                     AERGIESRLVPEAGFTITLLPGRGLQRRLTLQNVAAAFGLAVALARAIVLVRRRRPAV
                     VLALGGFASAACALAAVLWRVPLVLADQNARAGASNRLVARFARAAAVPFEETDLPGA
                     VVTGNPVRGEILAVAADPDPAAARRTLGLPDGRTVVVVFAGSLGSRRINEAVYGALSA
                     WTGRSDLAVHHVVGSRDWDDRPDAARREPSWGGPDGLWYRAVRYEDRMDLVLAAADLA
                     VCRAGGTTVAELAVLGVPAVLVPLPIATRDHQSA"
BASE COUNT          972 a         3034 c         2915 g         1177 t
ORIGIN      
        1 ggccccgagg acgcgacccc cgagcagaga tcgccgtcgc gtcccggcgc cgatggcaga
       61 cccggcccgc acccgccgcc ggctgtgggg gatggtcgcc gcgttcgggg tgctggccct
      121 gctcccgctg gtgaagctcg tcggcgtcca gttcgcggat gcctcgacgc tcgccgctga
      181 gggactgcag cagcgccagc gcaccgagtc gatccccgcg ccgcggggct cgatcctcga
      241 ccgcaacggc tcggagctgg ccatctcggt gccccgcacc accgtggcgg tcaacaaggt
      301 cgtcctcgcc cgggccggga tcagcgacga cgccatgctc cgggagttcg gaacccgcct
      361 ggcgcagttg ctcgacgtcg atcccgccga cgtcgtcgac tccctcctcg acgcggatgc
      421 cgatgcccgc tgggtccgcg tcgtgcgcta cgcggacgtc gacgtcgccc gcaaggccgc
      481 ccgctccctc gccgaacagg acatggacgg cgtgctcctg ctcgatccga catcggaaag
      541 ggtgcacccc ggtggcgaat ccggggtccg cgtcatcggc accgtcgggc cggacggccc
      601 cggcccgcgg gctgcggtcg aggccaagta cgacgacgtc ctcaaggggg ttcccggcga
      661 gcaggtcgtc gaacggggag cgcagggcga gacgatcacc ggcggcgacc gggtcgtgac
      721 cgccccccgc cccggggccg acgtccaggt caccctcgac cggacgcttc agtacgagac
      781 cgagcagata ctcaccaagg gatcggagaa cgcccgggcc gccggcggca tcgccatcgt
      841 cggacgaccc tcgaccggcg agctgctggc ggtcgccggc gtcgagcgca acgagaagac
      901 cggagcgatc gagctgtcgt cacggccgct cgcgttctcc gacgcctacc aggccggttc
      961 ggtgttcaag ctggtgacca ccgctgcggc ctacgaggcc ggcgtgatcg acgaccactc
     1021 gaccttcagc gtcggtcccc ggatcaaggt ctacgaccgc gagttcagcg acaacgaggc
     1081 ccacgccgtc gagacgatga acgtcgacca gatcgtggcg cactcctcca acgtcggaac
     1141 gatccagatc gcccagcgcc tcggggccga gaagctgcac gccgccctgg tcgacttcgg
     1201 cttcgggcgg gtctccgccc tcgacctgcc cgcggagagc accggtctgc tgcccgacgt
     1261 cgacaagtgg accccgcccg acaccgccgc ggcctcgatc ggatcgttcc agtccaccac
     1321 cgtgctgcag ttgtggtcgg cgttcaacgt gatcgccaac cagggcatct acgtgccccc
     1381 gcggctggtg cagggcacga tcggcgccga cggccggctc gacccggcct ccgcggcccc
     1441 gacccgtcgg gtggtctcgg cgtcgaccgc ggcgcgggtc agccgggcgc ttcaggccgt
     1501 ggtgcaggtc ggcaccggca agcagtggag cctgcccggc tacccggtcg cggccaagac
     1561 cggcaccggc cggatgccga gccccgagaa ggtcgacgcc aaggacgcct acgtctggcc
     1621 cgacggcacc taccaccacg tgaccacctt cgccgggtac ctgcccgcgg atcgtccgca
     1681 ggtgtcgatc accgtgatgc tcttcgacac cgcccagggc ctgaccggct cgacctctgc
     1741 cggtccggtg ttcagcgacc tgtcccggct ggcgatccgc gagctgtcga tcgccccgac
     1801 cacgaccgac gccgggtcgg cggacggtgc cggcccggtg cgctccgctc cggccgggtc
     1861 caccgccgcg gccaccgacc gggcgctgct gcccggtcgc aacggcgggg gctccaccag
     1921 ttcccgctcc ccgtcgacca ccattgctcc ctccggtcgg acgtcgcgtc cgggctccgg
     1981 acagcagcgg tagcgagacg agcgtggatc cacacgagag cgacggaaac gacgggacgc
     2041 aggagggtca cagcagcggc ggggctgccg cggccgtgac cgtcggcgag ctcctcgccg
     2101 cgctcgacgc cacgcacccc ggcgcacggc tccacggcga tgcctccgca tcggcggtcc
     2161 cgctccggtc ggtcacccac gactcacgcc gggtgggtcc gggcaccctg ttctgctgcg
     2221 tcgtcggcga acatgccgac ggacacgact tcgcagccga ggccgtggcc gcgggagcgg
     2281 tcgccctcct ggtgcagcgc gaccccgacc ccgacggtcc gctgcgctcg gttccgcaga
     2341 tcgtcgtcga cgacgtgcgc gccgccatgg cgacagccgc cgtcgaggtc taccgacacc
     2401 ccgcggagcg cctgcgcacc gtcggggtga ccggcaccaa cggcaagacg accgtcgtgt
     2461 ccgcggtcgc ccacgtgctg tccgcggcgg ggcgtcgggt cgaggcgatc ggcacgctga
     2521 ccggggctcg cacgacgccc gaggcccccg acctgcaggc ccggctggcc gagctggtct
     2581 ccgccggcgt caccgacgtg gccatggagg tgtcgtcgca cgccctgtcg ctgcaccggg
     2641 tcgacggcgt cgtgttcgac gtggccgtgt tcaccaacct cggccaggac cacctcgact
     2701 tccacgggac gcccgaggcc tacttcgcgg ccaaggccca gctgttcgag gcgggccggt
     2761 cccggcgggg catcgtcgac gtcgacgacg tccacgggcg actgctgatg gattcggctc
     2821 cgcccggtca cccgatggtg gcggtgtcgg tcgccgacgc ggagccggtc acctcgacgg
     2881 tgacggggtc gagcttccgg tggcggggac ggtcggtgag ctgcccgctc cccgggcgcc
     2941 acaacgtcgc caacgccatg ctcgcagccg aggcgtgcgc ggcgctgggc gtcgaggagg
     3001 actcgatcgt cgccgcgctc gcggacctgc cggtggtacc cggccgcttc gagcgggtcg
     3061 acgccggtca ggacttcggc gtcgtcgtcg actacgccca cacccccgac gcgctcgagg
     3121 cggtgctcgt cgccgcccgg gagctcggcc ccggtcgggt cctggtcgtg ttcggctgcg
     3181 gcggggaccg tgacgccacc aagcgcccgc ggatgggggc ggtcgcctcg gagcacgccg
     3241 atgtggtggt gctcaccacc gacaaccccc gctcggagga cccgcttcgc atcctcgagg
     3301 cggtccggtc aggatgcagc ggtgaccccg tcgtcgaacc cgaccggcgc gccgccatcg
     3361 cggtcgcgct cggatccgcc cgccccggag acgtggtcgt gatcgccggc aagggccacg
     3421 agcagggcca ggaggtcgcc ggcgtcgtca cccccttcga cgaccgcctc gtcgccgccg
     3481 agctgctcgc atcgacggag ggaccgtcgt gatccgtctc ctgctcgcca ccggcatcgc
     3541 cgccctggtc accctggtgg ggacccggct gctgatcggc gcgctcaccc gacgccacat
     3601 cggccagccc atccacgagg acgtccccga gggccacacg gtcaaggccg gcacgccgac
     3661 catgggcggc gtcgccatcg tggccggcgg gctgtgcggg tacctggcgt cgaacctcta
     3721 cgacggcgtg ttcacctgga ccggactggc cgccatgggc gcgatcgcag gcgccgggac
     3781 cgtcggcttc ctcgacgact ggatcaagat ccgcaacgag cggaacctgg gcctcaacaa
     3841 gcgatccaag accctgggcc tgctgctggt cgcggtcggc ttcgcgctgg tgatcctgtg
     3901 gcggaccaac gtccacacga cgatcagctt cggccgcttc gacgacctgg gctggaccct
     3961 cggcaagtgg gtgtgggtcg cgtgggcggt gctggtcatc ttcgcctcgt ccaacgccgt
     4021 caacctcacc gacggcctcg acggcctggc ggccgggtcg gccatcttcg tgttctcggc
     4081 ctacgtgatc atcggctact ggatcttccg gcacccgggc atgtaccaga tcaacgtcgg
     4141 actcgatctg gcggtcgtcg cggcggccat gatgggcgcg tgtgcggggt tcctgtggtg
     4201 gaacgccgcg ccggcgcaga tcttcatggg cgacaccggg tcgctggcca tcggcaccgg
     4261 tctggccgcg ctggcgctga gcagctcgac ggtggtgctg ctgcccatcc tcgggctgct
     4321 gttcgtcatg gagaccgtgt cggtgatcgt gcaggtggcg tcgttccgca ccaccggccg
     4381 gcgggtgttc cgcatggcgc cgatccacca ccacttcgag ctgggcggct ggcccgagac
     4441 caccgtgatc atccgggggt ggatgctcac cggcgccacc accgcggtcg gcctcgggct
     4501 gttctaccgg gagttcatcc gcgccggcgg tctcgcatga cctcgccggg gctcgccgag
     4561 cggtccctgc tcctgcacgg actcggactg accaacctcg cggtcgcccg cgccgcggtg
     4621 gcccacggtg tggccgtcgt ggcctccgac gactcgtcgc cggactcggc gtcgacgctg
     4681 gccgacgacc tcgggatcga ggtcgtgctc ggaccgtcgg ccgaccagct cgacgccctg
     4741 gtcgccggag tggacgcggt cgtgcccgct cccggactcc ccgagacgca cccgctgttc
     4801 gccgcggccg ccgcccacga ccggccggtg ctcagcgagt tcgacctggc cggcgcctgg
     4861 gacgaccggc ccgtcgtcgc catcaccggc accaacggca agaccacggt caccaccctg
     4921 gtgaccgcca tgttcgaacg ctccggggtg cgcgccgccg cggtcgggaa cctggagacc
     4981 ccgctggtcg aggcgatcgc ccgcgacgac atcgacgtgt tcgtcgtcga ggcgtcgtcg
     5041 ttccgcctgg cccacagccg ccgcttcgcc ccgagcgtgg gcgtgtggct gaacttcgcc
     5101 cccgaccacc tcgacgtgca ccgcaccctg gacgactacc ggcgggccaa ggcccggatc
     5161 tggcgggacc agaccgcggt cgacaccgcc gtggtcgtcg ccgaggaccc ggtggtcgac
     5221 gccgacgcgc tcgcccgggc cgacgcgccc gacgccccga ggttcgtccg cgtcgggctc
     5281 gacgccgtga tcgacgggag gccggtcccg ttccacgagc gagacggcgt gctggtcgga
     5341 gtcgccggtc ccgacgcgtc gcccgtcgat ctggtggcgg tggccgacct gtggcgttcg
     5401 ctgccgcacg accgcaccaa cgccctggcg gccgcggcgg ccgcgctggc cgggggagcg
     5461 tcgatcgacg gcgtccgcgc cgccctggtc gggttccggg gccttccgca ccgcgtcgag
     5521 ctggtcggaa cctccggcgg catccgctac tacgacgact ccaaggccac cgcgccgcac
     5581 gccacgctcg cggcggtctc cggcttcgag tcggtggtgc tcatcgccgg cggtcgcaac
     5641 aagggcctcg acctcggcga gctgcgacgc ggcaccgatc gcatccgggc ggtcgtcggc
     5701 atcggcgagg ccggccccga cgtgctcgac gccctggccg gtcggcccgg ccggccggcg
     5761 gactccatgg ccgccgcggt cgccgccgcg gccgacatgg cagagcccgg cgacgtcgtg
     5821 ctcctgtcgc cgggctgcgc gtcgttcgac tggtacggct cctacgccga gcgcggcgac
     5881 gacttcgcgt catgcgtccg ggcccgcgcc gacttcgagg agccgacgtg accgccaccc
     5941 gccgcggcgg ggcgggatcg tcgacgaagc ggggccggac cacggccgcg gcgcggagcg
     6001 gtcgggccgc tccgcctcgg cgtcccgaga tctcggtcgt ccggcgcgga ccggacccct
     6061 cgaccatggt cgtggtcacc gttgcgctgc tgtgcggtct cggcgtggtg atggtgtact
     6121 ccgcgtcgtc ggtcaccgcg gtgcgcaccc tcggccacag ctgggggatc gtgatgcgcc
     6181 aggccgtctg gctcgtcctc ggcgtcgcgg cggcctacgg ggcgtcgcgg gtcccgtggc
     6241 gcatctggcg cgaccgggtc gctctgcccc tgatgctggt ctcggtcgcc ctgctcggct
     6301 acgtcgccct cggcgtgctg gcgcagaagg ccaccggctc ggcgctgccg ttcgtgatct
     6361 cggtcaacgg cgccacccga tgggtcgggg cgggctcgat gcagttccag ccgtcggacc
     6421 tggccaagcc cgccctgatc ctgtggttgg cgcgcctgct gtcggagaag gggcgcgacg
     6481 tgcgcacctg ggagggcctg cgaccggtgc tgatctggtc ggggctgacc tgcggactcg
     6541 tcgtcttcgg cgacgacctc ggcacgacga tgctgctcgg cgcggtcacg ttggcgatgg
     6601 tgttcatggc cggtgcgccg ctgcccaagc tgggggcgat cgccggggtg tccatcggcc
     6661 tgggcggcct ggccatcgtc ggcctcgagc gcttccgggt gacgcgcatc ctggcgttcc
     6721 tcgaccccga caagtaccga cagggcgcgg ggtggcagct gtggcagtcg cagatcggcc
     6781 tggcgtcggg cggggtgttc ggcaccggac ccggcctggc gcgctccaag tgggggttcc
     6841 ttcccgaggc gcacaccgac ttcatcctgg cggtgatcgg cgaggagctc ggcctggtcg
     6901 gcagcctgat cgtgctcggg ctgttcgtgg tgttcatggc cgccggctgc gcgatcggga
     6961 tgcgcgcccg caccggcttc acccgactgg tcgccttcgg catcacgacc tggatcgggg
     7021 tccaggccct catcaacatc ggcgtcaccg tcggaaccct gccgaccaag ggcatcaccc
     7081 tcccgttcgt gtcctacggc gggtcgtcgc tgctgatgtc gctggtcggg gtcgggatcc
     7141 tgctgtcggt cgcccgggag gcctgaccgt gtcggccgcg cccccgacgt gggcgatcgt
     7201 cgccggcggc gggaccgccg gacacgtgtt gccgggtctg gcggtcgcac gggagctggt
     7261 cgaccgcggg catcgccccg agaccctgca cttcgtcggg gccgagcggg gcatcgagtc
     7321 gcggctcgtt cccgaggccg gattcaccat cacgctgctg cccggtcgcg ggttgcaacg
     7381 ccggctcacc ctgcagaacg tggccgccgc cttcggcctg gccgtcgcgc tcgcccgggc
     7441 gatcgtcctg gtgagacgtc gtcggccggc ggtggtcctg gccctcggcg gcttcgccag
     7501 cgcggcgtgc gcgttggccg cggtgctgtg gcgggtgccg ttggtgctcg ccgaccagaa
     7561 cgcccgcgcc ggagcctcca accggctcgt cgctcgcttc gcccgggccg cggcggtgcc
     7621 gttcgaggag accgacctgc ccggcgccgt ggtgaccggc aacccggtcc gaggagagat
     7681 cctggccgtg gcggccgacc ccgaccccgc ggcggcgcgc cggacgctcg gcctgcccga
     7741 cgggcgcacc gtcgtggtcg tgttcgccgg atcgctcggg tcccgacgca tcaacgaggc
     7801 ggtctacggc gccctgtcgg cgtggaccgg acgctccgac ctggcggtcc accacgtggt
     7861 cggctcccgg gactgggacg accgtcccga cgccgcccgc cgggagccgt cctggggcgg
     7921 gcccgacggt ctgtggtacc gggcggtgcg ctacgaggac cgcatggacc tggtcctggc
     7981 cgcggcggac ctggcggtgt gtcgggccgg cggcacgacg gtggccgagc tcgcggtgct
     8041 cggcgtgccc gcagtgctcg tcccgctgcc gatcgcgacc cgcgaccacc agagcgcg
//
LOCUS       DAOQOD010000138         8032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3324_length_8032_cov_11.769211, whole genome shotgun sequence.
ACCESSION   DAOQOD010000138 DAOQOD010000000
VERSION     DAOQOD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_8032_cov_11.769211"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLZ72_12185"
     CDS             complement(<1..544)
                     /locus_tag="PLZ72_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37100.1"
                     /translation="MTRRSPLSMRTGRGARGARGAVAAVGIACLVAAGVSCSGDDDTS
                     ARSSTTAPSTSVAAPSTVDDADFDKQAATAEAMIANAGNDPCRIVTAFAPASSLPTPI
                     NAEQTQRGVKVIAGLFTSAADSAPPAAAGDAAVLRQAATDLVAEGEAAQWSPDWLLKI
                     PKAISDAKVVQAFTNYQKAVA"
     gene            complement(541..1101)
                     /locus_tag="PLZ72_12190"
     CDS             complement(541..1101)
                     /locus_tag="PLZ72_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37101.1"
                     /translation="MRRRPILLPFIALLAVVAFVAGSCSSDDSADKDSDKTTTTKKSD
                     DDSEAGSSTVAPADFDAALSQAQKALEGAKDDPCKIMEAATTMSTAMSGATTPEQREQ
                     AAKIALDAIRAMADSASPELSAEADKLRDAADAIEKEGEQANWSEEFMQQPKAIKDNE
                     AYTEASSKITGELTQKCTPTSTTEAK"
     gene            1273..2169
                     /locus_tag="PLZ72_12195"
     CDS             1273..2169
                     /locus_tag="PLZ72_12195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNA37102.1"
                     /translation="MPGPDKRCTEDEVVAELRSGMTIGIGGWASRRKPMSIIRAILRS
                     DLTDLTVVAYGGPDVGLLCRSGQASKVISGFVSLDTIPLEPHYRAARQSGSIRAEELD
                     EGMFLSGLQAAAWRLPFLPTRAGLGSDVPRVMPHITTVRSPYPDPDTGEHEELIAAPA
                     LRLDVALVHQHRADASGNAVWLGEDPYFDDIMVRAAERSFVSCEKLLPTAELGEGTDV
                     THHSISRMDVTGVVEAPGGAHFTECLPDYGRDEAFQRLYAGSARSDEAWAEFSERFVQ
                     VDEETYRAHVRELAAAAEEGDK"
     gene            2166..2960
                     /locus_tag="PLZ72_12200"
     CDS             2166..2960
                     /locus_tag="PLZ72_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNA37103.1"
                     /translation="MSDPRRADVCAVAIAEAFRGDGESLCNPIGNLPLIGGRLAAATF
                     EPHLALTDGFYTLIDNVPAVGLSEDERQAQRVIGRWNPYREMFGLLWHGRRHVMMGAT
                     QVDRFGNQNIAAIGDWHQPRSQLLGFRGAPGNTINHTTSYWVPNHSPKVFVEAVDVVC
                     GIGYDRAAELGPTSSRFHEIRRVVSNLGVFDFVAAGSDGGDAVPTMRLASVHPGVSVD
                     QVVEATGFELQVPEHVPTSRGPEGAEVEELERIDPGGLRFREVPDE"
     gene            2957..4093
                     /locus_tag="PLZ72_12205"
     CDS             2957..4093
                     /locus_tag="PLZ72_12205"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNA37104.1"
                     /translation="MSTPTGPDAAPNGSEPARHPALSTRACDLFGVRYPLVQTGMGWV
                     SGARLTAATAAAGGLGILASATMTLDELGAAIDKVASRTSNPFGVNLLPNQPDAAERI
                     SLIVDRGVRVASFAAAPRPEQVQRLRDAGVVTIVTVGAKRHAQKVAEIGVSAVIAQGG
                     EGGGHTGSVPTSLLLPEVVDGVAGTDVVVLAAGGFSDGRGLAAALAWGADGVAMGTRF
                     LLTAESHVPDHVKDLYLGTPLTGTVVTTAIDGAPQRVIRTAMIDALERSPGLLRFPRA
                     AGNAWRFARQAGVSLPDLARSGVQMHKDQELSWSQVALAANAPMMTRASVVDGDVEVG
                     ILPTGQVVGVIDELPTVAGLLERIMDQADGALSRLCGPSPEPST"
     gene            complement(4053..6506)
                     /locus_tag="PLZ72_12210"
     CDS             complement(4053..6506)
                     /locus_tag="PLZ72_12210"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNA37105.1"
                     /translation="MTRDVVRRRRPVAITALIVASVSLLSVPVARALGLDASDDVVTR
                     LRIAADIAFSGGAAIALALVARRRHGTDRAVWGLLAAGCGAWCIGSVLWASYLARGSD
                     LPVPSPADAAYLVLPLAWTAAGVVRGARGQRDRPGRPLTIDLIDSAIVGLSLLIILWV
                     TAFHAGDIDARDTATLVTMVYPIGSAVAATLLTRSLADDTADRRAFTLVVAGITAYTV
                     GDVGFLVVTRTWAISDAVPTLVDTGWLVGFTLLAGAAWVAWPEPEEPRPRTRRTRRGL
                     LAVGLASTAAVVALVDLTRDDGTTWTISTLVAVMILMLTRQSLTLAENRRLSRDLVDR
                     IGALAHRADHDELTALANRSHLKERLQAVVHRSDAATPGAVMFLDIDMLKPVNDSLGH
                     DRGDELIRTVAGRLRDRFGDDVVRFGGDEFVVILRDRPSIEAILADAEETARDMRRPY
                     RSGGITLAPSVSVGVAVVEDDATPDELLRRADTALYRAKDTGRGRAALYDPSMDAVSA
                     RRLRIAPELRRAVDDGELELHYQPIVHLSTGRIQRVEALLRWRHPEKGILTPDRFLTE
                     ADALGLLPEIGRRSLLEGTCRIAEVNRRNPDRAVQIAVNLSASELGTDVLLGVDEALA
                     ASGLEPAMLVLEITEDVIVDESVRRTLAELRSRGVTIAIDDFGTGNSSLRQLGDYPAS
                     VLKIDRSFVDGIDGADDRLIVRAVVDLAAELDLITIGEGVETAEQAELLAAMGCVHGQ
                     GWLFDRAVPFETLERRWLQGPPSTARALRALGRTRSSTADGEAGLDPTGSTDPTERTA
                     SARSPSIRSTVPETVRTAD"
     gene            complement(6503..>8032)
                     /locus_tag="PLZ72_12215"
     CDS             complement(6503..>8032)
                     /locus_tag="PLZ72_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA37106.1"
                     /translation="RAEWVFVFAMLITLASVATQRLFLDALDPVLLLLGLVAVIASAG
                     RLALLQREQTDLQQQLGSIAEQLRVRARTDELTGLGNRLALVEEVDRVLLSGARDVHA
                     FYIDIDDFKTVNDALGHEVGDQLLVRTAEHLTAAFGPRTFRLGGDEFVALAVDATCAD
                     AAERARRAVAAATEPVDIGGVAVTARLSIGVSHATVDVDGTPSDSPEELLRMADLALN
                     RAKELGRCRVATFDTWLQERVDRRLMVQHGLHRAVELDEFEVSYRPEVDLATGEVLGA
                     EALLRWETPDHHLLHPAEYLGIAADSGLVPAISRSGLRRATAPWASADPPTVPLSINL
                     TRPELLHGGLLDDLEEIAGSIPRSALRLQIPEAAFMDPATRLTIDQLVRAGYGICVRD
                     FGTASSSIRRLSWLPDPAIRIDRSFVSGLAQRRSDRAVVSTIVEVGAALGVRITADGV
                     TRPGQVSMLRELGVTRGQGWLFGRPVPWPELIRRTDRSGSGSALRWQPPTAPLTTEVV
                     T"
BASE COUNT         1113 a         2821 c         2945 g         1153 t
ORIGIN      
        1 tggccacggc cttctggtag ttcgtgaagg cctggaccac cttggcatcc gagatcgcct
       61 tggggatctt gaggagccag tccgggctcc actgcgccgc ctcgccctcg gccaccaggt
      121 cggtggcggc ctgacgcagc accgcggcgt cgccggccgc ggccggtggc gcgctgtccg
      181 ccgcgctggt gaacagcccg gcgatcacct tcaccccgcg ctgggtctgc tcggcgttga
      241 tgggtgtcgg caggctggac gccggggcga aggcggtgac gatccggcac gggtcgttgc
      301 cggcgttggc gatcatcgcc tcggcggtag cggcctgctt gtcgaagtcc gcgtcgtcga
      361 ccgtcgacgg tgcggccacc gacgtgctcg gcgccgtggt cgacgatcgc gccgatgtgt
      421 cgtcgtcgcc ggagcaggag accccggcgg cgaccagaca ggcgatcccg accgcagcga
      481 cggcgccacg ggcgccacgt gcgcctcgac cggttcgcat cgacagcggg gagcgacggg
      541 tcacttggcc tcggtggtgg aggtcggcgt gcacttctgg gtgagctcgc cggtgatctt
      601 ggaggaggcc tcggtgtagg cctcgttgtc cttgatcgcc ttgggctgct gcatgaactc
      661 ctcggaccag ttcgcctgct cgccctcctt ctcgatggcg tcagcggcgt cgcgcagctt
      721 gtcggcctcg gccgacagtt ccggcgaggc ggagtcggcc attgcccgga tggcgtcgag
      781 tgcgatcttg gccgcctgct cacgctgttc gggagtggtg gccccggaca tggcggtgct
      841 catggtggtg gcggcctcca tgatcttgca cgggtcgtcc ttggcgccct cgagcgcctt
      901 ctgggcctgg ctcagggcgg cgtcgaagtc ggcgggcgcc acggtggacg aacccgcctc
      961 ggagtcgtcg tcggacttct tggtcgtggt cgtcttgtcg gagtccttgt cggcggagtc
     1021 gtcggagctg cacgacccgg cgacgaacgc gaccaccgcc aacagggcga tgaacggcag
     1081 gaggatgggg cgacggcgca tggtggtcct tggggggttc ggcgtcgaga tgtccctccg
     1141 acgctaccgg caccggcggt caggtgcgcg cgtcgccggg ttccttctca ggtggtccct
     1201 ccggtacggt cccatctgac gccgcgtcag aaagctcggt cgcggcggag ccgaccaccg
     1261 gaggagagac aggtgccagg gcccgacaag aggtgcaccg aggacgaggt ggtggccgag
     1321 ctgcgctcgg gcatgacgat cggcatcggc gggtgggcga gccggcgcaa gcccatgtcc
     1381 atcatccggg cgatcctccg ctcggatctg accgacctga ccgtcgtcgc ctacggcgga
     1441 cccgacgtgg gcctgttgtg ccgctccggt caggcatcga aggtgatctc cgggttcgtg
     1501 tcgctcgaca cgatcccgct cgagccgcac taccgggcgg cgcggcagtc cgggtcgatc
     1561 cgggccgagg agctcgacga gggcatgttc ctgtcgggcc tgcaggccgc ggcgtggcgc
     1621 ctgccgttcc tgcccacccg ggccggcctc ggctccgacg tcccccgggt gatgccgcac
     1681 atcaccacgg tgcgctcccc gtaccccgat cccgacaccg gcgagcacga ggagctgatc
     1741 gcggcgccgg ccctgcggct cgacgtcgca ctggtccacc agcaccgggc cgacgcctcg
     1801 ggcaacgccg tgtggctggg cgaggacccg tacttcgacg acatcatggt gcgcgccgct
     1861 gagcggtcct tcgtgtcctg cgagaagttg ctgcccaccg ccgagctcgg cgagggcacc
     1921 gacgtcaccc accacagcat ctcccgcatg gacgtgaccg gcgtcgtcga ggcgccgggc
     1981 ggggcgcact tcaccgagtg cctgcccgac tacggccgcg acgaggcgtt ccagcgcctc
     2041 tacgccggct ccgcccgctc cgacgaggcg tgggccgagt tctcggagcg gttcgtgcag
     2101 gtggacgagg agacctaccg ggcgcacgtc cgggagctgg ccgccgcggc agaggagggc
     2161 gacaagtgag cgatccccga cgggccgacg tctgcgccgt ggccatcgcc gaggccttcc
     2221 gcggcgacgg cgagagcctc tgcaacccga tcggcaacct gccgttgatc gggggccgac
     2281 tggcggcggc caccttcgaa ccgcacctgg cgctgaccga cggcttctac acgctgatcg
     2341 acaacgtgcc cgccgtcggc ctgtccgaag acgagcggca ggcccagcgg gtcatcggtc
     2401 gctggaaccc gtaccgcgag atgttcggtc tgctgtggca cggccggcgt cacgtcatga
     2461 tgggtgccac ccaggtcgac cggttcggca accagaacat cgccgcgatc ggcgactggc
     2521 accagccccg ctcgcagctg ctcgggttcc ggggcgcgcc cggcaacacg atcaaccaca
     2581 ccaccagcta ctgggtgccc aaccactcgc ccaaggtgtt cgtcgaggcc gtcgacgtcg
     2641 tctgcggcat cggttatgac cgggccgccg agctcggccc gaccagctcg cggttccacg
     2701 agatccgccg ggtcgtgtcc aacctgggcg tgttcgactt cgtcgccgcg ggctccgacg
     2761 gaggggacgc cgtccccacc atgcgcctgg cgtcggtgca ccccggcgtg agcgtggacc
     2821 aggtggtcga ggcgaccggc ttcgagctgc aggtccccga gcacgtcccg accagccgcg
     2881 gtcccgaagg tgccgaggtc gaggagctcg agcgcatcga tcccggcggg ctccgcttcc
     2941 gagaggtccc cgacgagtga gcaccccgac cggccccgat gctgctccga acggatcgga
     3001 gccggcccgc cacccggcgc tgagcacgcg ggcctgtgat ctgttcgggg tgcgctaccc
     3061 gctggtgcag accggaatgg ggtgggtctc cggtgcccgc ctgaccgcgg cgaccgcggc
     3121 ggccggcggt ctggggatac tcgcctcggc caccatgacc ctcgacgagc tcggtgcggc
     3181 gatcgacaag gtcgcgtcgc gcacgtcgaa cccgttcggg gtgaacctgc tgcccaacca
     3241 gcccgacgcc gcggagcgga tctcgctgat cgtcgaccgc ggggtgaggg ttgccagctt
     3301 cgcggccgcg ccgcggcccg agcaggtgca gcgcctccgc gacgccggcg tggtcacgat
     3361 cgtcaccgtc ggcgccaagc gccacgccca gaaggtcgcc gagatcgggg tcagcgccgt
     3421 gatcgcccag ggcggcgagg gtggcggtca caccggctcg gtgcccacgt cgctgctgct
     3481 gcccgaggtg gtcgacgggg tggcgggcac cgacgtggtc gtgctggccg ccgggggctt
     3541 ctccgacggt cgcgggctcg ccgccgccct ggcctggggg gccgacggcg tggcgatggg
     3601 gacccggttc ctgctcaccg ccgagagcca cgtccccgac cacgtcaagg acctctacct
     3661 ggggaccccg ctcaccggca cggtcgtcac caccgccatc gacggagccc cgcagcgggt
     3721 gatccgcacc gccatgatcg acgcgctgga gcgttcgccg ggcctgctgc ggttcccccg
     3781 cgcggctggc aacgcgtggc gcttcgcccg tcaggccgga gtgtcgttgc ccgacctggc
     3841 ccgctctggt gtgcagatgc acaaggacca ggagctgtcg tggtcgcagg tggccctggc
     3901 cgcgaacgcc ccgatgatga cccgggcgtc ggtcgtcgac ggcgacgtcg aggtcggcat
     3961 cctgccgacc ggtcaggtcg tcggggtcat cgacgagctg ccgacggtcg ccggactgct
     4021 cgagcggatc atggaccagg ccgacggagc cctcagtcgg ctgtgcggac cgtctccgga
     4081 accgtcgacc tgatcgacgg cgatcgcgcc gaggcggtcc gctcggtcgg gtcggtcgag
     4141 ccggtcgggt cgagcccagc ctccccgtcg gcggtgctgg accgggtccg accgagggcc
     4201 cgcagcgccc gggcggtcga cggcggcccc tgcaaccacc gccgttccag ggtctcgaac
     4261 ggcacggccc ggtcgaacag ccatccctgg ccgtggacgc agcccatcgc ggccaggagc
     4321 tcggcctgct cggcggtctc gaccccttcg ccgatcgtga tcaggtcgag ctccgcggcc
     4381 aggtcgacca cggcgcgcac gatcagtcgg tcgtcggcgc cgtcgatgcc gtcgacgaag
     4441 ctgcggtcga tcttgagcac cgacgccggg tagtcgccca gctgacgcag cgacgagttg
     4501 ccggtgccga agtcgtcgat ggcgatcgtc accccacgac tccgcaactc ggcgagggtc
     4561 cgacggacgg attcgtcgac gatcacgtcc tcggtgatct ccagcaccag catcgccggt
     4621 tcgagcccgc tggcggcgag ggcctcgtcg actccgagga ggacgtcggt gccgagctcg
     4681 ctcgcggaca ggttgaccgc gatctgcacc gcccgatccg ggttccggcg gttgacctcg
     4741 gcgatgcgac acgtcccctc gagcagggat ctccgtccga tctccggcag gagcccgagg
     4801 gcgtcggcct cggtcaggaa ccgatccggg gtcaggatgc ccttctcggg gtgacgccac
     4861 cgcagcaggg cctcgacccg ttggatccgt ccggtggaga ggtgcacgat cggttggtag
     4921 tgcagctcga gctctccgtc gtcgacggct cggcgcagct ccggggcgat ccgcagccgc
     4981 cgggccgaca cggcgtccat cgacgggtcg tagagcgccg ctcgtccccg gccggtgtcc
     5041 ttggcccggt acagcgcagt gtcggcgcgt cggaggagct cgtcgggggt ggcgtcgtcc
     5101 tccacgacgg cgacgccgac cgataccgac ggcgccaggg tgatccctcc gctgcggtac
     5161 ggccggcgca tgtcccgggc cgtctcctcg gcgtcggcca ggatcgcctc gatcgacgga
     5221 cggtcccgga ggatcaccac gaactcgtcg cccccgaagc ggaccacgtc gtcgccgaag
     5281 cgatcccgga ggcggcccgc cacggtccgg atgagctcgt cgccgcggtc gtggcccagc
     5341 gagtcgttga ccggcttcag catgtcgatg tcgaggaaca tgaccgcccc cggggtggcg
     5401 gcgtccgatc ggtggacgac ggcctggagt cgctccttga ggtgcgagcg gttggccagg
     5461 gccgtcagct cgtcgtgatc ggcacggtgc gcgagagcgc cgatccggtc gaccaggtcg
     5521 cgtgacaggc gtcggttctc ggccagggtc agcgactgac gggtgagcat caggatcatc
     5581 accgcgacga gcgtgctgat cgtccaggtc gtgccgtcgt cccgggtgag gtcgaccagt
     5641 gccaccaccg cggcggtcga cgccaacccg accgccagca gccctctgcg ggtccgacga
     5701 gttctcggcc gaggttcctc gggttccggc caggcgaccc atgccgcgcc ggcgagcagg
     5761 gtgaagccga cgagccagcc ggtgtcgacc agggtgggga cggcgtcgct gatcgcccac
     5821 gtgcgggtga cgaccaggaa tccgacgtcg ccgaccgtgt aggcggtgat ccccgccacc
     5881 accagggtga acgcccgccg atccgcggtg tcgtcggcga gggaccgggt cagcagcgtg
     5941 gcagccacgg cggatccgat cggatacacc atcgtcacca gcgtcgccgt gtcacgtgcg
     6001 tcgatgtcgc cggcgtggaa cgcggtgacc cagaggatga tgagcagcga cagccccacg
     6061 atcgcgctgt cgatcagatc gatcgtcagg ggacgtcccg gtcggtcccg ctggccccgg
     6121 gctccccgga cgactccggc cgcggtccat gccaacggga ggacgaggta cgcggcgtcg
     6181 gccggactgg ggacgggcag atcggaccca cgggcgaggt aggacgccca caggaccgat
     6241 ccgatgcacc acgcgccgca ccccgcggcg agcaggcccc acaccgctcg gtcggtgccg
     6301 tgccgacgcc gggcgaccag agcgagggcg atcgctgcac cgccgctgaa cgcgatgtcc
     6361 gccgcgatcc gcagtcgggt gacgacgtcg tcgctcgcgt cgagacccag agctctcgcg
     6421 accgggaccg acagcagtga gaccgaggcg acgatcagag ccgtgatcgc caccggtcgc
     6481 cgtcgacgca cgacgtcccg ggtcacgtga ccacctcggt ggtcagcgga gcggtcgggg
     6541 gctgccatct cagagcggag cccgaaccgc tccggtcggt ccggcggatg agctcgggcc
     6601 acgggaccgg tcgtccgaac agccagccct gccctcgggt cacgccgagc tcccgcagca
     6661 tcgacacctg accgggccgg gtcactccgt cggcggtgat ccggacaccg agggccgcgc
     6721 cgacctcgac gatcgtcgag acgacggccc ggtcggagcg acgttgcgcg aggcccgaca
     6781 cgaacgaccg gtcgatccgg atcgccggat cgggtagcca gctcagccgc cggatcgacg
     6841 aggatgcggt gccgaagtcc ctcacgcaga tcccgtagcc ggccctgacc agctggtcga
     6901 tggtcagtcg cgtggcgggg tccatgaacg cggcttcggg gatctgcagt cgcagcgcgg
     6961 agcgcgggat cgagccggcg atctcctcga ggtcgtcgag caggccgccg tggagcagct
     7021 cgggacgggt gaggttgatc gacagcggaa cggtcggagg gtccgccgag gcccacgggg
     7081 ccgtggcccg acggaggcca gaccggctga tcgccgggac cagacccgag tccgcggcga
     7141 tcccgaggta ctcggccgga tggaggaggt ggtggtccgg ggtctcccag cggagcaggg
     7201 cctcggcgcc gagcacctcg ccggtggcca ggtcgacctc gggtcggtag ctcacctcga
     7261 actcgtcgag ctcgacggcc cggtgcaggc cgtgctggac catcagccgc cggtcgaccc
     7321 gttcctggag ccaggtgtcg aaggtggcca cacggcaccg gccgagctcc ttggcccggt
     7381 tcagggccag gtcggccatc cgaagaagct cttcgggtga gtccgacggg gtgccgtcga
     7441 cgtcgacggt ggcgtgggag acgccgatgc tcagccgggc ggtcacggcc acgccgccga
     7501 tgtcgaccgg ctcggtggcg gcggccacgg cccgtcgggc ccgttccgcc gcgtcggcac
     7561 aggtcgcgtc caccgcgagc gcgacgaact cgtcgccgcc gagccggaag gtgcgcgggc
     7621 cgaacgctgc ggtcaggtgc tcggcggtcc ggaccagcag ctggtcgccg acctcgtgac
     7681 cgagggcgtc gttgaccgtc ttgaagtcgt cgatgtcgat gtagaaggcg tggacgtccc
     7741 gggcgccgga gagcagcacg cgatcgacct cctccaccag ggccagccgg ttgccgagac
     7801 cggtgagctc gtcggtccgg gcccggacac ggagctgttc ggcgatcgac cccagctgct
     7861 gctggaggtc ggtctgctcc cgctggagca gcgcgagtcg tccggccgat gcgatcaccg
     7921 cgaccaggcc gagcaggagg aggaccgggt cgagcgcgtc gaggaacagg cgctgcgtgg
     7981 cgaccgacgc gagggtgatc agcatcgcga acacgaagac ccactcggcc cg
//
LOCUS       DAOQOD010000139         8028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3328_length_8028_cov_12.518375, whole genome shotgun sequence.
ACCESSION   DAOQOD010000139 DAOQOD010000000
VERSION     DAOQOD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_8028_cov_12.518375"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..2195
                     /locus_tag="PLZ72_12220"
     CDS             486..2195
                     /locus_tag="PLZ72_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37107.1"
                     /translation="MLSRIITARGTRVAVAAALVATLFAFSAGSPTSAAPQTKTLTGS
                     CSGADAASAGLLAAFGGTLNMPFQITSDVPAQLDPEADDQPISFTWSVTLAASVTSQV
                     AAIDPSLTIKDITLDMGVTGPTETKVVEGRPAPLNVNVTAGQPATFAMGPFAGQLTGI
                     GKGGVIKYSPKAIGLTISIDIGGKATDVKVNCAAAGTAAITSIKIPGSPDIKQPIELE
                     GTANSSVTVDVLGEYVTPGKTEDGKEMPIKPETLKVVDGPGQVADGKVVVNTGAAGTT
                     SSVTFEVCSGELPGTNEVQTITLDTTGETFKKGVAFTLKYGEEVTAPINMIDETSLPV
                     ILNPGLVNIPTNDWMNNANNYFLVGFQLPAPGDIQSALERLPSIGGGGVKVTAGEKPG
                     SYDVEFVGKNGQKDVDSLAIDHYYSVFPQEALAGIIDAAKGLLGGGGGEGPTTTTTLP
                     GGAADLDGAIAWLTAQINAEPIFSPRWQELFGQRASLEMKKAMANIDVNAAIAMLTGL
                     FSTPPNAVTTTAGEAPIGICSQGIIDVTVPAAAPTAVAGASTPGADVAGANQTGASLV
                     LAG"
     gene            2406..2873
                     /locus_tag="PLZ72_12225"
     CDS             2406..2873
                     /locus_tag="PLZ72_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37108.1"
                     /translation="MAPSTTTAPPIRAHAAPGSPVVLRHRRAVARWALASGRPVHRDA
                     LAAIIGARADSLSAPADLDGPTEWVGEQIGELLWGDISSWCGERSARVPEPDDIARTL
                     HTYLRYLSAHRLLAAGSDPVTLLRRAIADYGGDRGRHRHPSTGRRVAPVVPIA"
     gene            complement(2905..3909)
                     /locus_tag="PLZ72_12230"
     CDS             complement(2905..3909)
                     /locus_tag="PLZ72_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37109.1"
                     /translation="MTPRNRTVWILVGIVGVLVMVVVVLAVALIGSDDGATTSSLPAR
                     RPTTSTTPAVTSVPPSVPAAPTSTTSTPAGAVSVQDVTVQEGIVQRQYLVVRPVDVGA
                     DERLPTVMVLHGLTVDRFAMAATAPWAEAVARDRFVAVFPQGILSSWNAGPCCPPATL
                     ASVDDVGFLTRVVDQLRVRPDVDPDRLYLTGFSNGGMMAYTLACSRTEDFTAVAPMAA
                     TNVSGCSPSQPISLLHMHGDPDPTVPYDGSPSVSQLLSSQPFPAVPASVAAWAEADGC
                     GTDPQRRSPTDGVTIDSWPDCPRGIRVELITYPGNGHAWPTAPLDGLDVMLRFFGISG
                     "
     gene            4031..5236
                     /locus_tag="PLZ72_12235"
     CDS             4031..5236
                     /locus_tag="PLZ72_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37110.1"
                     /translation="MESTATQIDEQEFRREVADWMAEHLTGDFAILRHRGGPGDEHAF
                     VEERMEWERELASGGWTGVGWPVEHGGRGLPIALEVAFHEEYARAGGPGRAGLIGEGL
                     LGPTLIHFASPQIQERFLPGIVSGTEFWCQGYSEPNAGSDLANVSTRARLEGDEWVID
                     GQKVWTSLAQWSDWCFVVARTEEGSTRHHGLSYLLVPMDQPGIEVRPIRQITGTAEFN
                     EVFFDGARTAADLVVGAPGDGWKVAMGTLAFERGVLTLGQQMAFDRELGHIVDAARRT
                     GAIDNPVTRQRLVQLWARVRIMRWNAVRALRVDENVALPREAMINKLYWAELHRDMGE
                     VAMDVLGLEATTTRYSEADEGDAGEQEMSALHKLFFFSRSDTIYGGSNEIQRNVIGER
                     ALGLPREPR"
     gene            5321..6106
                     /locus_tag="PLZ72_12240"
     CDS             5321..6106
                     /locus_tag="PLZ72_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37111.1"
                     /translation="MPAAPTPIEPPGRGLLDGKRVVITAAAGTGIGGALARRCLMEGA
                     TIVISDAHERRIGETADALGDEFGTRPEAVVCNVTDNSDVQALVSTAVDRMGGIDVWM
                     NNAGLGGNGLVTELTDEEWDRVIDVTLTGTFKCLRAVLPVMYEQGSGAVVNNASVLAW
                     RAQERQSHYAAAKAGVMALTRCSAIEAAEHGVRINAVSPSLAMHANLAKVTPPGLLEE
                     LTSKEAFGRAAEPWEIANVMVFLASDLSSYMTGEVLAVSSQHP"
     gene            6219..6692
                     /locus_tag="PLZ72_12245"
     CDS             6219..6692
                     /locus_tag="PLZ72_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNA37112.1"
                     /translation="MTVVAEPTALLTMVGHDLGTTEWTTVTQDRIALFADATGDHQWI
                     HVDVERATAESPFGGPIAHGYLTLSMVNLFLPELLTVERFSMGVNVGLEKVRFPAPVP
                     SGSRIRARGEVVASSEEKGGVQVTVRVTVERDLDGSGTPDAKPACVADTVSRFFP"
     gene            6827..8026
                     /locus_tag="PLZ72_12250"
     CDS             6827..8026
                     /locus_tag="PLZ72_12250"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNA37113.1"
                     /translation="MTEAVIVATARTPIGRMSKGSLKDMRPDDLSAHIITSVLDKVPA
                     LDRTEIEDVLWGCGQPGGESGHNIARVSAVLAGLDDVPGATVNRYCSSSLMTIRMAAH
                     AIKAGDGDVFVAGGVETASRFPFGMADGPKNPVFADAEAHSAERAQGGAPVWAAQAGL
                     PDMYLAMGQTAENVAQLENVSRQDQDEFAVRSQNLAEESLNNGYWEAEITPVTTPDGV
                     VTTDDGIRPGTTYEKVSGLSPVFRPDGTVTAGNACPLNDGAAAVVVMSDTKAKALGLT
                     PLARIVSSAATGLNPEIMGLGPVEAVRKALGRAGMTVDDIDLFEINEAFAAQVLPSAR
                     QLGIPMEKLNTRGGSIALGHPFGMTGARIMTTLIHNLQESDKTFGVETMCVGGGQGMA
                     MVVERLS"
BASE COUNT         1305 a         2939 c         2696 g         1088 t
ORIGIN      
        1 ggacgcggcc gttgtggacg cggccgttgt ggacgcggcc gggccacccg gctcgtcggg
       61 gccgccgccc cgcgtcgtcg caccgtccgc cacttgtctc ccagcactcc ccggcaacga
      121 gcgcagccgg tgctccggcg ccgctcgggt tccaagggta cacatcccac gaagtcggcc
      181 cggggaggcg atgccccagc gacgacaccg ctccgatcac ccccaccgcc ggcccgccgc
      241 accccaatcc gtcaccctcc gtagcacggc gaccgcggtg ggtagaacgg ggatggggaa
      301 tcatacccgc ggacccctgg accccttgcg catggtcacc cgaaacgcct attcaatccc
      361 cacacacgtg tgtcgtccga ctcgtcgctg tgcggcgcgg tgggcggtcg gagccgttcg
      421 ggacctctcc ggacagctcc gtctgggggc tgcaggcgac accgatcgac ggtctggagg
      481 gctgcatgtt gtcgaggatc atcacagcgc gtgggacacg ggtggcggtc gccgccgcgc
      541 tggtcgccac actgttcgcg ttctcggcgg gatcgccgac ctccgcggcc ccgcagacca
      601 agacgctcac cggctcctgc agcggagccg acgcggcgtc ggccggactg ctcgccgcct
      661 tcggcggcac cctgaacatg ccgttccaga tcacctcgga cgtcccggcg cagctcgacc
      721 ccgaggccga cgaccagccg atctcgttca cctggtcggt cacgctcgcc gcctcggtca
      781 cctcgcaggt cgcggcgatc gacccgtcgc tgaccatcaa ggacatcacg ctcgacatgg
      841 gtgtcaccgg cccgaccgag accaaggtcg tcgagggtcg tccggctccg ctcaacgtca
      901 acgtgaccgc cggtcagccg gcgacgttcg cgatgggccc gttcgccggt cagctcaccg
      961 gcatcggcaa gggcggcgtc atcaagtact cgcccaaggc catcggcctg accatctcga
     1021 tcgacatcgg cggcaaggcc accgacgtca aggtcaactg cgcggctgcg ggcaccgcgg
     1081 cgatcacctc gatcaagatc cccggctcgc ccgacatcaa gcagcccatc gagctcgagg
     1141 gcacggccaa ctcgtcggtc accgtcgacg tcctcggcga gtacgtcacc cccggcaaga
     1201 ccgaggacgg caaggagatg ccgatcaagc ccgagaccct caaggtggtc gacggccccg
     1261 gtcaggtcgc cgacggcaag gtcgtcgtca acaccggcgc agcgggcacc accagctcgg
     1321 tcaccttcga ggtgtgctcg ggcgagctgc ccggcaccaa cgaggtccag acgatcaccc
     1381 tcgacaccac cggcgagacc ttcaagaagg gcgtggcgtt cacgctgaag tacggcgagg
     1441 aggtcaccgc tccgatcaac atgatcgacg agacctcgct gccggtgatc ctcaaccccg
     1501 gcctggtcaa catcccgacc aacgactgga tgaacaacgc caacaactac ttcctggtcg
     1561 gcttccagct cccggccccc ggcgacatcc agtccgccct cgagcggctg ccgtcgatcg
     1621 gcggcggcgg ggtcaaggtg accgcgggtg agaagcccgg cagctacgac gtcgagttcg
     1681 tcggcaagaa cggccagaag gacgtcgaca gcctggcgat cgaccactac tactcggtgt
     1741 tcccgcagga ggcgctggcc ggcatcatcg acgccgccaa gggcctgctc ggcggcggcg
     1801 gcggcgaggg cccgaccacc acgaccaccc tgcccggcgg cgccgccgac ctcgacggcg
     1861 ccatcgcatg gctcaccgcc cagatcaacg ccgagccgat cttcagcccc cgttggcagg
     1921 agctgttcgg ccagcgagcc agcctcgaga tgaagaaggc gatggccaac atcgacgtca
     1981 acgccgccat cgccatgctg accggcctgt tcagcacccc gccgaacgcg gtgaccacca
     2041 ccgccggtga agccccgatc gggatctgct cccagggcat catcgacgtg accgtcccgg
     2101 ccgcggcgcc gaccgctgtc gccggagcct ccacgccggg agcggacgtg gccggggcca
     2161 accagacggg agcgtcgctg gtactcgccg gctgatccca ttcgagcacg agggtggatc
     2221 tgatcgggtc gggcgggtca tcccgcccga cccgatccgc gtccccaccc accccgccac
     2281 ccaaccccgc gttctgacga caagtcacga cactggcggt actcaactgt catcggaatc
     2341 gtgctcgcac ggccagggga ccagcggccg cggccactgt cacacccccc gaccacgatg
     2401 gggccatggc tccgagcacc acgaccgcac caccgatccg agcccatgcc gcccccgggt
     2461 cgcccgtggt tctccggcat cgacgggccg tcgcccgatg ggcgctggcc agcggacgac
     2521 cggtgcaccg cgacgccctg gccgcgatca tcggcgcccg cgccgactcg ctgtcggccc
     2581 ctgccgacct cgacggtccc accgagtggg tgggcgagca gatcggcgaa ctgctctggg
     2641 gtgacatctc gtcctggtgc ggcgagcgct cggcccgggt cccggagccg gacgacatcg
     2701 cccgcaccct gcacacgtac ctccgctatc tgtccgcgca tcgcctgctc gcggcgggct
     2761 cggatccggt gaccctgttg cgacgagcga tcgccgacta cggcggtgat cggggtcgcc
     2821 accgacatcc ctcgacgggt cgacgcgtcg ctccggtcgt gccgatcgcc tgacctgact
     2881 ggtttttccg gtcaggcaga tcggtcagcc gctgatgccg aagaagcgca acatgacgtc
     2941 cagcccgtcc agcggggcgg tcggccatgc gtgcccgtta ccggggtagg tgatcagctc
     3001 cacccggatc ccccgcggac aatcgggcca ggagtcgatc gtgaccccgt cggtcggcga
     3061 gcggcgttgg ggatcggtac cgcagccgtc ggcctcggcc cacgccgcga ccgatgccgg
     3121 cacggccgga aacggttggg acgacagcag ctggctgacc gacggcgacc cgtcgtaggg
     3181 gacggtcgga tccgggtcgc cgtgcatgtg cagcagcgag atcggctggc tcggcgagca
     3241 accggacacg ttggtcgccg ccatcggtgc aacagcagtg aagtcctcgg tgcgggaaca
     3301 cgccagggtg tacgccatca tcccgccgtt cgagaacccg gtgaggtaga gccggtccgg
     3361 gtcgacgtcc ggacggaccc gcaactggtc gaccacccgg gtcaggaatc cgacgtcgtc
     3421 gaccgatgcc agggtcgccg gcggacagca cggacccgcg ttccacgacg agaggatccc
     3481 ctgggggaac accgcgacga accggtcgcg ggccaccgcc tcggcccacg gagcggtggc
     3541 agccatggcg aagcgatcga cggtgaggcc gtgcagcacc atgacggtgg gcaggcgctc
     3601 atcggcaccg acgtcgacgg gccgcaccac caggtactgc cgctgcacga tgccctcctg
     3661 cacggtgacg tcctggaccg acaccgcccc ggcgggggtg gaggtggtcg acgtcggcgc
     3721 cgccggcacc gacgggggaa cggaggtgac cgccggggtc gtgctggtgg tcggacgacg
     3781 ggcgggcagg ctgctcgtcg tggcgccgtc gtcggagccg atcagagcca cggccaggac
     3841 caccaccacc atcaccagga cgccgacgat cccgaccagg atccacaccg tccggttccg
     3901 cggggtcacg gcgggcgagt ctacgggcac ccgcgatcgg gtcggcggcg cccggatcgg
     3961 gtcgggtggc gtcgatcgac ttccggtcgc gctttctgac gggtcgtcag ttccatctat
     4021 cgtcgggacc gtggaatcga ccgcgaccca gatcgacgag caggagttcc gccgggaggt
     4081 cgccgactgg atggcggagc acctgaccgg ggacttcgcc atcctccgcc accggggcgg
     4141 gccgggcgac gagcacgcct tcgtcgagga gcgcatggag tgggagcggg agctcgcctc
     4201 cggcggatgg accggggtgg gttggccggt cgagcacggc gggcgaggac ttccgatcgc
     4261 cctcgaggtc gcgttccacg aggagtacgc ccgagccggc ggccccggcc gggccggtct
     4321 catcggcgag ggccttctcg gcccgacgct gatccacttc gcatcgccgc agatccagga
     4381 gcggttcctg cccggcatcg tgtcgggaac cgagttctgg tgccagggct actccgaacc
     4441 caacgccggc tcggacctgg ccaacgtctc gacacgggcg cgcctcgagg gcgacgagtg
     4501 ggtcatcgac ggccagaagg tgtggacgtc gctcgcccag tggtcggact ggtgcttcgt
     4561 cgtggcccgc accgaggagg gatcgacccg ccaccacggc ctgtcgtacc tgttggtgcc
     4621 gatggaccag cccggcatcg aggtgcgacc gatccggcag atcaccggaa ccgccgagtt
     4681 caacgaggtg ttcttcgacg gggcccgcac cgccgccgac ctggtggtgg gagcaccggg
     4741 cgacggctgg aaggtcgcca tgggcacgct cgcgttcgaa cggggcgtgc tcaccctcgg
     4801 ccagcagatg gccttcgacc gggagctcgg ccacatcgtc gacgcggcac gccgcacggg
     4861 cgccatcgac aacccggtga cccgccaacg cctggtgcag ctgtgggcgc gggtccgcat
     4921 catgcgctgg aacgcggtgc gggcgctgcg ggtggacgag aacgtcgcgc tgccccgcga
     4981 ggcgatgatc aacaagctct actgggccga gctgcaccgc gacatgggcg aggtggccat
     5041 ggacgtgctg ggtctcgagg cgaccaccac ccggtactcc gaggccgacg agggtgacgc
     5101 cggcgaacag gagatgtcgg cgctgcacaa gctgttcttc ttcagccgct ccgacacgat
     5161 ctacggcggc tcgaacgaga tccagcgcaa cgtcatcggg gaacgggccc tcggactccc
     5221 ccgcgagccc cgctgagacc ccacccgcac agctctgtcc cgcacagcgg gcgtgatcac
     5281 ccccgctctg caggacagag cgggcgaaga ggagacacca atgcctgcag caccgacacc
     5341 gatcgaaccc cccggacgag gtctgctcga cggcaagcgg gtggtcatca ccgcggcagc
     5401 cggcaccggc atcggcggag ccctggcccg acggtgcctg atggagggtg cgaccatcgt
     5461 catctccgac gcccacgagc gacgcatcgg cgagaccgcg gatgccctcg gcgatgagtt
     5521 cggcacccgc cccgaagccg tcgtgtgcaa cgtcaccgac aactccgacg tacaggcgct
     5581 cgtctccacc gcggtcgacc gcatgggcgg catcgacgtg tggatgaaca acgccggcct
     5641 gggcggcaac gggctggtca ccgagctgac cgacgaggag tgggaccggg tcatcgacgt
     5701 gacgctgacc ggcacgttca agtgcctccg agcggtgctc ccggtcatgt acgagcaggg
     5761 ctccggcgcc gtggtcaaca acgcctcggt gttggcgtgg cgcgcccagg agcgccagtc
     5821 ccactacgcc gcggccaagg ccggtgtgat ggcgctgacc cgctgctcgg cgatcgaggc
     5881 ggccgagcac ggcgttcgca tcaacgccgt cagcccgagc ctggccatgc acgccaacct
     5941 ggccaaggtg acgccgccgg ggttgctcga ggagctcacc tcgaaggagg cgttcggccg
     6001 ggccgcggag ccgtgggaga tcgccaacgt catggtgttc ctggccagcg acctgtcgag
     6061 ctacatgacc ggcgaggtcc tcgccgtctc cagccagcac ccctgacccc acaccccgag
     6121 aacccacccc accccacccc cgaacccgtt ctgtagtgcg agatccaggc gttcgcctgg
     6181 atctcgcact acagaacggc cattgccagg agatcgtgat gaccgtcgtt gccgaaccca
     6241 ccgccctgct caccatggtc ggtcacgacc tgggcaccac cgagtggacc accgtcaccc
     6301 aggaccgcat cgcactgttc gcggacgcca ccggcgacca ccagtggatc cacgtcgacg
     6361 tcgaacgggc cacggccgag tcgccgttcg gcgggccgat cgcccacggg tacctgaccc
     6421 tgtcgatggt caacctgttc cttcccgagc tgctgaccgt cgagaggttc tcgatggggg
     6481 tcaacgtcgg actcgagaag gtccggttcc ccgcgccggt cccctccggg tcccggatcc
     6541 gagcccgcgg cgaggtcgtg gcctcctccg aggagaaggg cggggtgcag gtcacggtcc
     6601 gggtcaccgt ggagcgtgac ctcgacggct ccggcacgcc cgacgccaag ccggcgtgcg
     6661 tggccgacac cgtcagccgc ttcttcccct gaggggacct cagcaggttt gtcccgagga
     6721 gccccgccac cccggcgatt gggagccatg gttaccgatc ggtaacctgc ggtccgccag
     6781 aagagccgtc gaccccgacg ccggggtccg cagaagggga ttcgccatga ccgaggccgt
     6841 gatcgtcgcc accgcccgca cgccgatcgg acgcatgtcc aagggctcgc tcaaggacat
     6901 gcgtcccgac gacctgtcag cccacatcat cacgtccgtc ctggacaagg tgccggcgct
     6961 cgatcgcacc gagatcgagg acgtcctgtg gggctgcggc cagcccggcg gcgagtccgg
     7021 ccacaacatc gcccgggtct cagcagtgct cgccgggctc gacgacgtcc ccggcgccac
     7081 ggtcaaccgc tactgctcgt cgtcgctgat gaccatccgc atggccgccc acgcgatcaa
     7141 ggccggcgac ggcgacgtct tcgtcgccgg cggcgtcgag accgccagcc gcttcccgtt
     7201 cggcatggcc gacgggccca agaaccccgt cttcgcagat gcggaggcgc actccgccga
     7261 gcgcgcccag ggcggtgccc cggtgtgggc ggcccaggcc ggcctgcccg acatgtacct
     7321 ggccatgggt cagaccgccg agaacgtcgc ccagctcgag aacgtcagcc gccaggacca
     7381 ggacgagttc gcggtccgca gccagaacct ggccgaggag agcctgaaca acggctactg
     7441 ggaggccgag atcactccgg tcacgacccc cgacggcgtc gtgaccaccg acgacggcat
     7501 ccgacccggc accacctacg agaaggtcag cggcctgtca ccggtcttcc gtcccgacgg
     7561 caccgtcacc gccggcaacg cctgcccgct caacgacggt gccgccgcgg tggtcgtgat
     7621 gagcgacacc aaggccaagg cgctggggct gactcccctc gcccgcatcg tctcctcggc
     7681 ggccaccggc ctcaaccccg agatcatggg cctcggtccg gtcgaggcgg tccgcaaggc
     7741 cctgggccgg gcgggcatga ccgtcgacga catcgacctg ttcgagatca acgaggcgtt
     7801 cgccgcccag gtgctgccgt cggcccgtca gctcggcatc ccgatggaga agctgaacac
     7861 ccgcggcggc tcgatcgccc tcggtcaccc gttcggcatg accggcgccc gcatcatgac
     7921 gacgctgatc cacaacctgc aggagtccga caagaccttc ggcgtggaga ccatgtgcgt
     7981 cggcggcggc cagggcatgg cgatggtcgt cgagcgcctc agctgacc
//
LOCUS       DAOQOD010000140         7909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3407_length_7909_cov_9.455946, whole genome shotgun sequence.
ACCESSION   DAOQOD010000140 DAOQOD010000000
VERSION     DAOQOD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_7909_cov_9.455946"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLZ72_12255"
     CDS             complement(<1..696)
                     /locus_tag="PLZ72_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37114.1"
                     /translation="MQPRPLRIRRIVVALMAIAATLLPALGAPGWSSPAAAQGTCGNT
                     DATPRLTVTPTALPAAATSTITVTGSDFLVPPHPCGATVFGGLYLFFGWVAPGGQWGP
                     SWRNSTDSAGQFGVTFSYPGEGGGGDTRDDGTGLVRLISFTPGGTSGSETPFHLDGGG
                     GFSTTLTVRGALYSWTDISTGRTNTVDCRTVQCGVFTIGAHGKSSRTNEQFVPVTFRT
                     DGPAPTVAPGGVSA"
     gene            complement(753..1619)
                     /locus_tag="PLZ72_12260"
     CDS             complement(753..1619)
                     /locus_tag="PLZ72_12260"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HNA37115.1"
                     /translation="MSVATTLRGLRRDVRRPDRSATPGPAIRARGIGVVLDGASVLYD
                     VDVDVDTGHLLALVGPNGAGKSTLLSVLAGDRQPERGDVEIDGLPVGSWSAVEMAMRR
                     AVLLQQVTMSFPFTVRQMVEMGRAPWVGQPEEADDERRVVESMAATDVSHLAERTVPS
                     LSGGERARAALARVLAQDTGILLLDEPTAALDVRHQEQVLALASDAAASGRAVVVVMH
                     DLGLAAAHADRVVVLAGGSVAADGAPADVLTAPLLSEVYEHPIDVLRHPQDGRLLIVP
                     GHDRPSTTIRHA"
     gene            complement(1616..2728)
                     /locus_tag="PLZ72_12265"
     CDS             complement(1616..2728)
                     /locus_tag="PLZ72_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNA37116.1"
                     /translation="MTTAVRPGVERTHATDAPGVGRRRPRPMALLVALGVALVAAAIV
                     AAAVGQLPIAPSEVLGSVLHRVGGWVGLDIGSVPSHPRGEAALWNVRFPRVLLAALVG
                     AALGGAGAVLQAVFGNPLAEPSVIGVSGGAAVGACSVIVFGWSFLGSYTVPAIAFATA
                     LGTTLVIYAMARHGGRTEVVTLVLMGIAVNAVTAAVIAYLVFLGDSQAREQIVFWQLG
                     SFNGSRWEAVRVVAPVVAVGLAGVMAITRQLDLLALGERSARHLGVNVERLRLTSIVI
                     VSLLVASGVAFVGIIAFIGLIVPHLVRMVVGPSHRTLVPASVLGGALLVVIADVVART
                     AVDYADLPIGMLTALVGGPFFFWMLHRTRTAAGGWG"
     gene            complement(2725..3645)
                     /locus_tag="PLZ72_12270"
     CDS             complement(2725..3645)
                     /locus_tag="PLZ72_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNA37117.1"
                     /translation="MTDRSTPVLPTTVTDFTGESVTVTDAERILALDTYGTLATTVYA
                     LGLGDRLVGRDISTGVPTLQDLPVVTHNGHELNAEAILELDPSVILTDYSIGPLEVQL
                     QLKEAGIPVVIMDDQRNRAVIGDQIRAVAEVLGVPTAGETLAASVAEDISRAESEVTA
                     MAEAAADPRMVFLYLRGNAGVYHWFGKGSGADDLIAALGGVDVAGESGLEGSRPLNAE
                     GLVRSEPDLYLMMSDGLGSVGGADGLLEIPGVADTAAGVDGCVVDMDDAQILSFGPQF
                     PVTLRALGDAVYGPDGVLGAGSESDGTEPS"
     gene            4092..4592
                     /locus_tag="PLZ72_12275"
     CDS             4092..4592
                     /locus_tag="PLZ72_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37118.1"
                     /translation="MAALTVATLVAVGCVADPGPTAGAALTAATSTVGVVDGSVVRVS
                     GRGYNPAANIGTRPPLFAQPAGVYAIFACVSDPWRPSQGATGSSRQIIQQFWAVPGQA
                     QFDAIGGAAAGAVLMGADGSFDVDVTLTAAPCTGRYAVITFPGSGAAANPGEENEIPV
                     TFAAPA"
     gene            complement(4893..5048)
                     /locus_tag="PLZ72_12280"
     CDS             complement(4893..5048)
                     /locus_tag="PLZ72_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37119.1"
                     /translation="MKRNDDILTGTAIAAPASRALRRARKAAVVSEPRLQIGFDRRSG
                     EYSLALL"
     gene            complement(5174..6616)
                     /locus_tag="PLZ72_12285"
     CDS             complement(5174..6616)
                     /locus_tag="PLZ72_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNA37120.1"
                     /translation="MSILVIDLGTSGVRAAIVGADASVTHEFRSPTLPDSPAPGLVEF
                     DANAYAAAALDCARSALDAHGPVDAVGVTNQRGSTIVWDRATGEPLAPAQGWQDLRTV
                     GDCLVLAADGIRLAPNQSATKLANILDAVDPDRTRDLCFGTPDSWIVWKLTEGAHHVS
                     DLTNAAIWGLLSPDASHYDVTVMDRLRIPASILPRIVDSSGPIGEATALPGAPMVCGI
                     AGDQQASLIGQGCVRPGSAKITFGTGGMLDVCVGPQPPTVAGRAGAGTFPIVCWRRGQ
                     ETVWGVEAVMLSAGTNVEWLVEDLGVIERPEDSGALAASVPDADGVVYVPALLGLGTP
                     RWDYGARGTLLGLTRGSGRAHVVRAVLEGVAERGADLVDAAEADAGVTLSSLRIDGGM
                     SDNEVFVQHLADATQRPVEVSPVREATALGAGFLAGLAIGTWSGWDHVADTWTPSRRV
                     EPGTPTDRDRWREALDRAAGWHEDLSALDF"
     gene            complement(6677..7108)
                     /locus_tag="PLZ72_12290"
     CDS             complement(6677..7108)
                     /locus_tag="PLZ72_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37121.1"
                     /translation="MQPQIRSLPPMSPPQAYWRGTVTALVVALPAGVMNQVLVSGGDI
                     DPGSPWTFLFWILIMLGGAAGGWAVRRLAPGSHLAWAAGAAATAYVVVQAIGVVRRLV
                     TGDPISWIAYPFLALLMATCGVMGAAYAGRMTRRYGEGEQG"
     gene            complement(7108..>7909)
                     /locus_tag="PLZ72_12295"
     CDS             complement(7108..>7909)
                     /locus_tag="PLZ72_12295"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNA37122.1"
                     /translation="STTVAVALLVAASFLDGVAQGAMWVLAILFDVGGPFLFGSAGWR
                     LVPGHFAERHSLILIIALGESIVAIGVGAEHVGIDAPVVAVAVLGVFLSAALWWLHFD
                     VVALATERRLVAMVPGQAQNALARDAYSYLHLLLVAGIVLVALGLKTVIAHVADPLPI
                     EVATALAGGVALYLVGHVMFRWRFARSINRERLGVAALLTALVPLSTSVPGWLALAAV
                     AAVTWALVIYETTAWSDTRRLIRDEHGMPGQDAAVDSPSQNPADDPEV"
BASE COUNT         1184 a         2814 c         2917 g          994 t
ORIGIN      
        1 ggcgctcacc ccgccggggg cgacggtggg tgccggaccg tcggtgcgga acgtcacggg
       61 gacgaactgc tcgttggtgc ggctggactt gccgtgggcg ccgatggtga acactccgca
      121 ctgcaccgtc cggcagtcga cggtgttggt ccggccggtg gagatgtcgg tccacgagta
      181 cagcgccccg cgcacggtga gggtcgtgga gaagccgccg ccgccgtcga ggtggaacgg
      241 cgtctcggat cccgatgtac caccgggggt gaagctgatc aggcgcacca ggccggtccc
      301 gtcgtcgcgg gtgtcgccgc caccaccctc gcccgggtac gagaacgtca cgccgaactg
      361 cccggccgag tcggtgctgt tgcgccagct cgggccccac tgcccgccgg gggccaccca
      421 gccgaagaac aggtacaacc cgccgaacac cgtcgccccg cacgggtgcg gggggaccag
      481 gaagtccgat ccggtgacgg tgatcgtcga tgtggccgcg gcggggaggg cggtcggagt
      541 gacggtcagc cgaggggtgg cgtcggtgtt cccgcaggtg ccctgcgcgg cagcggggct
      601 cgaccaaccg ggtgccccga gggcgggcag gagcgtggcc gcgatcgcca tcagggcgac
      661 gacgatccgt cggatacgca gtggacgggg ctgcatcgcc gagaggttag gcatccctaa
      721 tattcgaatg tcaaacgcga tttgaagccg catcaggcat gcctgatagt cgtcgagggc
      781 cggtcatggc cgggaacgat gagcaaccgt ccgtcctgcg ggtggcgcag cacgtcgatc
      841 gggtgctcgt agacctcgct cagcagcggt gcggtcagga cgtcggccgg cgctccgtcg
      901 gcggcgacgg acccacccgc gagcacgacc acccggtccg cgtgcgccgc ggccagcccg
      961 aggtcgtgca tgacgaccac gaccgcccgg cccgacgcgg ccgcgtcgga cgccagcgcc
     1021 aaaacctgct cctggtggcg gacgtcgagc gcggcggtcg gctcgtcgag caggaggatc
     1081 ccggtgtcct gggcgagcac ccgggccaac gcggcccggg cccgttcccc gcccgacagc
     1141 gacgggacgg tccgctccgc caggtgcgac acgtcggtcg cggccatcga ctcgacgacc
     1201 cgacgctcgt cgtcggcctc ctcgggctgg cccacccacg gagcccggcc catctccacc
     1261 atctggcgca cggtgaaggg gaacgacatg gtcacctgct gcagcaggac cgcccggcgc
     1321 atggccatct ccaccgcgct ccacgagccg acggggagcc cgtcgatctc gacgtcgccc
     1381 cgctccggct gtcggtcccc ggcgagcacc gacagcagcg tcgacttgcc cgctccgttg
     1441 ggacccacca gcgccagcag gtgaccggtg tcgacgtcga cgtcgacgtc gtacagcacc
     1501 gatgcaccgt cgaggaccac gccgatcccc ctcgcccgga tcgccggtcc gggggtcgcg
     1561 ctgcggtcgg gtcggcgcac gtcgcggcgc aggccccgca gggtggtcgc gacgctcacc
     1621 cccagccccc ggccgcggtg cgggtccggt gcagcatcca gaagaagaat ggcccgccga
     1681 ccagagcggt gagcatcccg atcggcagat cggcgtagtc caccgcagtg cgggcgacga
     1741 cgtcggcgat caccaccagg agcgccccac cgaggacgct ggcgggcacg agcgtccggt
     1801 gcgacggtcc gacgaccatc cgcaccaggt gcgggacgat cagcccgatg aacgcgatga
     1861 tgccgacgaa cgccaccccg gacgcgacga gcagcgacac gatcacgatc gatgtcaggc
     1921 gcagccgttc gacgttcacc cccaggtgcc gtgccgagcg ctcgcccagg gcgagcaggt
     1981 ccagttgccg ggtgatcgcc atgacgccgg cgagcccgac cgcgacgacc ggggcgacga
     2041 cgcggaccgc ctcccaccgc gagccgttga agctccccag ctgccagaac acgatctgct
     2101 cacgggcctg gctgtcgccg aggaacacca ggtaggcgat gaccgcggcg gtgaccgcgt
     2161 tgacggcgat gcccatcagc accaacgtga ccacctcggt gcggccaccg tggcgggcca
     2221 tggcgtagat gaccagcgtg gtgccgagcg ccgtggcgaa ggcgatcgcc ggcacggtgt
     2281 agctgccgag gaagctccag ccgaacacga tcaccgagca cgccccgacc gcggcacctc
     2341 cggacacccc gatcacgctg ggctcggcca acgggttgcc gaacaccgcc tgcaggaccg
     2401 ctccggcccc accgagggcg gcgccgacga gcgcggcgag cagcacccgg gggaaccgga
     2461 cgttccacag agccgcctcg ccccgtgggt gcgacggcac ggagccgatg tcgagcccga
     2521 cccacccccc gacccggtgc agaaccgacc ccagcacctc cgacggcgcg atcgggagct
     2581 gaccgaccgc cgcggcgacg atcgccgcgg ccacgagcgc caccccgagc gcgacgagca
     2641 gggccatcgg gcgtggccgg cgacgcccga ccccgggagc gtcggtggca tgggtgcgtt
     2701 cgacacccgg cctgaccgcg gtggtcacga cggctccgtg ccgtccgact ccgaccccgc
     2761 cccgagcacc ccgtcggggc cgtagacggc gtcgccgaga gctctcagcg tgaccgggaa
     2821 ctgcggaccg aagctcagga tctgggcgtc gtccatgtcg acgacgcagc cgtcgacgcc
     2881 cgccgcggtg tcggccaccc cggggatctc gagcagcccg tcagcaccgc cgaccgagcc
     2941 cagcccgtcg ctcatcatca ggtacaggtc gggctcggag cggaccagcc cctcggcgtt
     3001 gagcggccgg gagccctcga ggccggactc gccggccacg tcgacgccgc cgagcgcggc
     3061 gatgaggtca tcggccccgg accccttgcc gaaccagtgg tagacccccg cgttgccccg
     3121 caggtagagg aagaccatcc gggggtccgc cgccgcttcg gccatcgcgg tcacctcgga
     3181 ttcggcccgt gagatgtcct cggccaccga cgcggccaac gtctcgcccg ccgtcggcac
     3241 accgaggacc tcggcgaccg cccggatctg gtcgccgatc accgcgcggt tgcgctggtc
     3301 gtccatgatc acgacgggga tgcccgcttc cttcagctgc agctgcactt cgagcgggcc
     3361 gatcgagtag tcggtcagga tgaccgacgg gtccagctcg aggatcgcct cggcgttgag
     3421 ctcgtggccg ttgtgggtga ccaccggcag gtcctgcagc gtggggacgc cggtcgagat
     3481 gtcccgaccc accaggcggt cacccagccc gagcgcgtac accgtcgtgg ccagggtccc
     3541 gtaggtgtcg agagcgagga tgcgctcagc gtcggtcacg gtgaccgact cgccggtgaa
     3601 gtcggtcacc gtggtcggca ggacgggggt cgaccggtcc gtcaccggct cgacccagtc
     3661 ctgggcgacc gcgtgtcgcg ggccccggca caccacgtcg tcggtggagc cgcgggagcc
     3721 ggcggtcgcc gcggtcgagg agccaccgca ggacgttgcg aggacgagcg cggcgacggc
     3781 ggcgctgagc agcgtcgttg tggttcggcg ccggcccggc ccccgatgac ggggtcgcgg
     3841 tgacggtgtg acggcggctg gttccggaga aggggtctgc acctggccaa tcattggagg
     3901 atcggtatgg caatgacaag ttgaccagca aacttgggaa caaatgttag ggatggctaa
     3961 cttgccgcac cgacaccgaa ccggaggtac cgatggacaa ccccactgcc gcaaccgagc
     4021 agacaggatc gacccgaccg agcagcaccg gggcacaccg acgcccgagc cgcaggtggg
     4081 cggcgtcgtc gatggcggct ctgacggtcg cgaccctggt ggcggtcggc tgcgtggccg
     4141 atcccgggcc gacggcgggt gccgccctga ccgcggcgac gtcgaccgtc ggggtcgtcg
     4201 atggctcggt cgtgcgcgtg agcggccgtg gctacaaccc ggcggccaac atcgggaccc
     4261 gacccccgct cttcgcccag ccggccgggg tgtatgcgat cttcgcctgc gtgtctgatc
     4321 cctggcggcc gtcgcagggg gccaccggct ccagtcggca gatcatccag cagttctggg
     4381 cggtccccgg gcaggcccag ttcgacgcca tcgggggtgc ggccgccggt gcggtgctga
     4441 tgggggccga cgggagcttc gacgtcgacg tgactctcac cgccgcgccg tgcaccggcc
     4501 gctacgcggt gatcacgttc ccgggcagcg gggcagccgc caacccgggt gaggagaacg
     4561 agatcccggt cacgttcgcc gcgccggcct gaggttccgg caacccgacc gtcgagcgcc
     4621 gtcgaacccg tcgtcgaact tgtagccagt gggtcgtgtg gtggcctgtg ggctacaagt
     4681 tcggcggggt gcgacgatct tgtagctggt gggtcgtggg gcggcctgtg ggctacaagt
     4741 tcggcggggg cgggccgggg gcggagcccg agaagcagac cggcgacggg aggccgcgct
     4801 cctcccgtcg ccgatcggat tggtggggcg agatcgcccc cggacgtgtt cagtggaatg
     4861 ggtcccgggg gacgtgtccc aaggggagcg ggtcagagga gggcgaggct gtactcgccg
     4921 ctgcgtcggt cgaagccgat ctgcagtcga ggttcggaga ccactgcggc cttccgggcc
     4981 cgccggaggg cccggctggc cggcgcggcg atcgctgttc cggtgaggat gtcgtcgttg
     5041 cgcttcatgt ggatgtctac ggcgggtccc ggcggttcct gaaggccgtc gggaccgtcg
     5101 ccccagaggg aagggtcgtc cggcccagcc cccgaaccgg tggcccagac gcctccgagc
     5161 ggacgtccga cgctcagaag tcgagcgcgg acaggtcctc gtgccatccc gcggcgcgat
     5221 cgagggcctc acgccagcgg tcgcggtcgg tcggcgttcc gggctcgaca cgccgcgacg
     5281 gggtccacgt gtccgcgacg tggtcccacc cggaccacgt gccgatggcc aggccggcca
     5341 ggaaaccggc ccccagggcc gtcgcctcgc ggaccggtga cacctcgacg ggccgctggg
     5401 tggcatcggc cagatgctgg acgaacactt cgttgtcgga catgccgccg tcgatgcgca
     5461 ggctggacag ggtgacgccg gcgtcggcct cggccgcgtc gaccaggtcg gctccccgct
     5521 cggcgacgcc ctcgaggacc gcccgcacga cgtgcgcccg tcccgatccc cgggtgagcc
     5581 cgagcagggt cccgcgggcg ccgtagtccc accgcggcgt tcccaggccg agcagcgccg
     5641 ggacgtacac gacaccgtcg gcgtcgggca ccgaggctgc cagcgccccg gagtcctcgg
     5701 ggcgctcgat cacccccagg tcctcgacca gccactcgac gttggtcccc gcggacaaca
     5761 tgaccgcctc gacgccccag acggtctcct ggccccgccg ccagcacacg atcgggaacg
     5821 tgcccgcacc ggcccgtccg gcgacggtcg gcggctgcgg acccacgcac acgtcgagca
     5881 tgccgccggt gccgaaggtg atcttggccg agccgggccg cacgcagccc tgcccgatca
     5941 gcgacgcctg ctggtcgccg gcgatcccgc acaccatcgg cgcccccgga agggcggtgg
     6001 cctcgccgat cgggcccgac gagtcgacga tgcggggcag gatcgacgcc gggatccgca
     6061 accggtccat caccgtgacg tcgtagtgcg aggcgtcggg cgacagcagc ccccagatcg
     6121 cggcgttggt gaggtccgag acgtgatgcg cgccctcggt caacttccac acgatccacg
     6181 agtccggtgt gccgaagcac aggtcccggg tgcggtccgg gtcgaccgcg tcgaggatgt
     6241 tggcgagctt ggtcgccgac tggttgggag ccaggcggat gccgtcggcc gcgaggacga
     6301 ggcagtcgcc gacggtgcgg aggtcctgcc agccctgggc gggggcgagc ggttcgccgg
     6361 tggcccggtc ccacacgatc gtcgagcccc gctggttggt gacaccgacg gcgtcgaccg
     6421 ggccgtgggc gtcgagtgcg gaccgggcgc agtcgagcgc ggcggccgcg taggcgttcg
     6481 cgtcgaactc gaccaggccg ggagcggggg agtccggcag cgtcggcgag cggaactcgt
     6541 gggtgaccga ggcgtcggca ccgacgatcg cggcccgtac cccgctggtt cccaggtcga
     6601 tcaccaggat gctcacgtcg ccccctcgtc ggtggtggtg gatcgggctg tcggatgcgg
     6661 ccggggccgc tgtgggtcag ccctgttcgc cctcaccgta gcgacgggtc atgcggcccg
     6721 cgtacgccgc gcccatgacg ccgcaggtgg ccatgagcag cgccaggaac gggtacgcga
     6781 tccagctgat cgggtcgccg gtgacgagcc gtcggacgac gccgatcgcc tggaccacga
     6841 cgtacgcggt ggccgccgct ccggcggccc aggccaggtg ggagccgggg gcgaggcgtc
     6901 gcacggccca gcctcccgcc gcgccgccca gcatgatcag gatccagaac aggaacgtcc
     6961 acggcgagcc gggatcgatg tcgccgccgc tgaccagcac ctggttcatc accccggcgg
     7021 gcagggcgac cacgagcgcg gtgaccgtgc ctcgccaata ggcctggggt gggctcatcg
     7081 gcggcaggga gcggatctgg ggctgcatca gacctcgggg tcgtcggcgg gattctggga
     7141 ggggctgtcg acggccgcgt cctgcccggg catgccgtgc tcgtcccgga tcagtcggcg
     7201 ggtgtcggac caggcggtcg tctcgtagat caccagagcc cacgtcacag ccgcaaccgc
     7261 ggccagggcg agccagccgg gcaccgaggt gctcagggga accagggcgg tgagcagggc
     7321 ggccacaccc agccgttcgc ggttgatgct gcgagcgaag cgccatcgga acatcacgtg
     7381 gccgaccagg tacaacgcca ccccgcccgc gagggcggtc gcgacctcga tcgggagggg
     7441 gtcggccacg tgcgcgatga cggtcttgag cccgagggcc accagcacga tcccggccac
     7501 cagcagcagg tggaggtagg agtaggcgtc gcgggccagg gcgttctggg cctggcccgg
     7561 caccatggcg accagtcgtc gctcggtcgc gagggcgacc acgtcgaagt gcagccacca
     7621 cagcgcggcg gacaggaaca cgccgaggac cgcgaccgcg accaccgggg cgtcgatgcc
     7681 gacgtgctcg gcgccgacgc cgatcgccac gatcgactca ccgagggcga tgatgaggat
     7741 gaggctgtgg cgctcggcga agtggcccgg caccagtcgc caaccggcgg acccgaacag
     7801 gaacggtccg ccgacgtcga acaggatcgc cagcacccac atggccccct gcgcgacgcc
     7861 gtcgaggaac gacgccgcga cgagcagggc gacggcgacc gtcgtgctg
//
LOCUS       DAOQOD010000141         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3492_length_7802_cov_10.986834, whole genome shotgun sequence.
ACCESSION   DAOQOD010000141 DAOQOD010000000
VERSION     DAOQOD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3492_length_7802_cov_10.986834"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLZ72_12300"
     CDS             <1..588
                     /locus_tag="PLZ72_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNA37123.1"
                     /translation="PPVIPAGRPARPTGELNAESMANALGALLPEGAVVADEGNTTGL
                     FAQGATAGCPPHDWLTLTGGAIGFGLPTATGAAVAVPGRKVICLEADGSAMYTAQSLW
                     TQVREGLDVTTIILNNGSYAVLNMELSRVGAGDPGPRALSMLDIPGLDFVALAASMGV
                     PATRATTAEEFTVQLEAAIAVDGPALVEAVVPRTI"
     gene            631..1575
                     /locus_tag="PLZ72_12305"
     CDS             631..1575
                     /locus_tag="PLZ72_12305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA37124.1"
                     /translation="MPPTDLEETRAPEPGLADVRRARSARRLRAVLVVTALVVVVVAG
                     GLAVVATRSGEGTGGTGSSAGGEGPSSERSRSTASTSTPTTAPTTTAPPVPTSVPGVT
                     VGPLVGGPGPIPVVHRVPTTDPVVFITIDDGAFADEDALKLIGEHRIPVTLFLNQSYF
                     AANADYFRRLQDSGAVLGAHTSTHAELRGRDAAFQHAEICDMVAEFRDRFGRAPTLFR
                     PPYGSYDATTLQQAASCGFTAALHWSAVADSGSIVTADGPLRAGDIVLLHFRPGLVLR
                     LYTVLTQIQAAGLRPASLSDYLDSAPPAPPAAPTTGPG"
     gene            1579..3627
                     /locus_tag="PLZ72_12310"
     CDS             1579..3627
                     /locus_tag="PLZ72_12310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNA37125.1"
                     /translation="MGRPDPSELLTRVIESMDSGEDRPQQRQMVEAVDRAFRTRTHLA
                     VQGPTGVGKSVAYLLPAITSARSHADARTIVVTSSKALQDQLGERDLPFLADALDVPF
                     RFAVLKGRSNYLCQAAVAETRVQLGRGEEQQTLDLGGGWDGDGSVPEVRAPDPMLRVE
                     LDRLLEWAGTTPTGELAELADPPSDAAWSALSVGPGECVGASRCGYAAECFSEQARER
                     AGGADIVVVNAHLYAADVQAGGQLLPAHDHLVIDEAHEFEDAMVGALGVSMTGWRIRR
                     LAQVHDRCVADAPTVAMAIGAAADAVDDALEQVFGEVSSDRGSGRLTGELPDVIADAL
                     TRADLAVDRAVTSLREVHKAAGNAPMSAAAHRFERAIRNCDGVSDDLHALRDDLHPGQ
                     VRWISEGRRGRFALNLTRIDIGPTLRTTAWERPVDDDSPDAATDDGDGSGGPTVVMCS
                     ATLDPGTAGRLGLRAEYLAVDSPFDFRRHGLLYVPRLPRPNAEAWPDAVVDELVHVIE
                     RCGGRTLALFTSHRMLRRSVDAVRDRLPDADVLAQGDAPNRVLQERFLANEHASLFAT
                     ASFWTGISSPGTTCSAVVVDKIPFPVPTDPIVEARCDAVGADRAFMEVSVPAAGVQLA
                     QGVGRLIRTATDRGVVAVLDPRLAEARYRARILDRLPRMKRTRERGDLDEFIDSLGLG
                     "
     gene            complement(3628..5259)
                     /locus_tag="PLZ72_12315"
     CDS             complement(3628..5259)
                     /locus_tag="PLZ72_12315"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37126.1"
                     /translation="MSRAASTTPAPVGSLRPIGTTGYGGRGERERAAAVRAALAAGAP
                     TPRVAIIGAGAAGLCMAIRLRDAGIDSFTIYEKSDGVGGTWRDNTYPGAACDVPSHLY
                     SFSFASKRDWTRRFAGQPEILGYFESLVDRFDLSGHLRLGTEVTEAHYDDATGTWTLR
                     TATSDGEPSEGTAAGTVVADVVVSGLGQLNRPYVPDIPGLDAFVEGGGTVFHSARWDH
                     DHDLSEDRVGVVGIGASAIQFVPPVAASAAHTTLFQRSANYVGPRNDREFTPRERWIF
                     ENVPGAQRAYRESIYWRFEARFNVMRAGSRLGRWMQGQFRKGLQPMVSERLPEAALVP
                     DYPVGCKRILIADDWYPTLLRPDVDVVTSGIERIEDGAVLTADGRRTPVDTLIFGTGF
                     RSTEFLSPLKITGRSGRDLNEVWVDGARAYMGLAVPGFPNLFMLYGPNTNLGHNSILF
                     MIEQQVGYTLRLIEEKVLLGLRSVEVSEGAASRFDTEVQAAADRTVWAEGCHSWYKNA
                     DGRITNNWTDHTTVYRRLLSSPDPSDWLLERAGSD"
     gene            5435..5797
                     /gene="trxA"
                     /locus_tag="PLZ72_12320"
     CDS             5435..5797
                     /gene="trxA"
                     /locus_tag="PLZ72_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNA37127.1"
                     /translation="MATTELTDDTFETTVKGDGIVLVDFWAAWCGPCRSFAPVFESAS
                     EDNPDITFAKVDTEANQQLAGALQIMSIPTLMMFRDGILLFSQPGALPRAALDDLISQ
                     ARELDMDQVRAEIDDRAS"
     gene            complement(5831..6160)
                     /locus_tag="PLZ72_12325"
     CDS             complement(5831..6160)
                     /locus_tag="PLZ72_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37128.1"
                     /translation="MSRARKISSDLRHVATLAGTTAAVVEGAAIAAERARSVADHLGG
                     DREQESAPVAELEVAARTCRGRRTLLVLVVVGLLAAVGVVVWKKRRSTEDEIADLRDD
                     RFAGSMP"
     gene            complement(6260..6538)
                     /locus_tag="PLZ72_12330"
     CDS             complement(6260..6538)
                     /locus_tag="PLZ72_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37129.1"
                     /translation="MGASRTSFAKLQRDRAKKAKAAAKRERRLTGENEIDDEPVTDVA
                     ELPEGEDLSADQLLRLVEELHADFEAKRIDFDTFEERKMELFARITVD"
     gene            6853..>7802
                     /locus_tag="PLZ72_12335"
     CDS             6853..>7802
                     /locus_tag="PLZ72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HNA37130.1"
                     /translation="MTLGSSLVVSRSDLDHMEVRPSVLTDGSTPPTGSALLAIESFAL
                     STHTVTTAASADSTGAWRHFPAPDGFGHIPTFGRARVARSGHPDLAEGLEVTGYLPMA
                     THLLVTPERIDQRGFSDGAPHRRGSAGVHDRYLFRAVDPTSVAADDPDLEVALRPQFT
                     MAFLIVAGLSARRMDEVAAVVVTSASCRTALGVAHLLGALEHPPAVIGVTRDDHVADT
                     AASGCYDRVLSYRDVASLPVGPTMVVDFCGDGAVAHEIHHHLGDSLLRSIVVGATHRN
                     AVPVDTAALPGATREIFLAPEVAEDLRRRVDDAAIEVAFT"
BASE COUNT         1041 a         2877 c         2748 g         1136 t
ORIGIN      
        1 ccgccggtca tcccggccgg tcggccggct cggcccaccg gcgagctcaa cgccgagtcc
       61 atggccaacg ccctgggcgc cctgctgccc gagggagcgg tcgtcgcgga tgagggcaac
      121 acgaccgggc tgttcgccca gggcgccacc gccggctgcc cgccccacga ctggctgacc
      181 ctgaccggcg gagcgatcgg cttcggcctg ccgaccgcga caggagctgc ggtcgccgtc
      241 ccgggtcgga aggtcatctg cctggaggcc gacgggtcgg ccatgtacac cgcccagtcg
      301 ctgtggacgc aggtgcgcga ggggctcgac gtgaccacga tcatcctcaa caacggctcc
      361 tacgccgtgc tcaacatgga gctgtcccgg gtcggtgccg gcgatcccgg tcccagggcg
      421 ctgtcgatgc tcgacatccc cggcctcgac ttcgtcgccc tcgcggcgtc gatgggggtg
      481 ccggcgaccc gggcgacgac ggccgaggag ttcaccgtcc agctcgaggc ggccatcgcg
      541 gtcgacggtc ccgccctggt cgaagcggtc gtcccccgca ccatctgagg cgtcctgtcg
      601 gcccggccca cgggccctaa cctgaccgcc gtgcccccca cggatctcga ggagacgcgc
      661 gccccggagc cggggttggc cgacgtccgc cgcgcccgat ccgcccgacg gctccgggcg
      721 gtcctggtcg tgacggcact ggtcgtcgtg gtcgtggcgg gcggactggc cgtcgtcgcg
      781 acgcgttcgg gcgagggcac cggcggcacc gggtcctccg ccggcggtga ggggccgtcg
      841 tcggagcggt caaggtccac cgcgtcgacg agcaccccca cgacggctcc cacgacgacg
      901 gcgccgccgg tccccacgtc ggtgccgggg gtgacggtcg gcccgctggt cggcggtccc
      961 ggtcccatcc ccgtcgtgca ccgggtgccc accaccgacc cggtcgtctt catcaccatc
     1021 gacgacggcg ccttcgccga cgaggacgcg ctgaagctga tcggtgagca ccggatcccg
     1081 gtgacgctgt tcctgaacca gtcgtacttc gcggcgaacg ccgactactt ccgccgactg
     1141 caggactccg gagcggtcct cggggcgcac acctcgaccc acgccgagct gcgcggccgg
     1201 gacgctgcgt tccagcacgc ggagatctgc gacatggtcg ccgagttccg cgaccggttc
     1261 ggacgggcgc ccacgctgtt ccgtccgccg tacggcagct acgacgccac caccctgcag
     1321 caggccgcca gctgcggctt caccgcggcg ctgcactggt cggcggtggc cgacagcgga
     1381 tcgatcgtca ccgccgacgg accgctccgc gccggtgaca tcgtcctgct gcacttccgg
     1441 cccggcctcg tgctgcgcct ctacaccgtc ctcacccaga tccaggccgc ggggctccgt
     1501 cccgcgtcgc tgagcgacta cctcgactcg gctccacccg ctcccccggc cgcgcccacc
     1561 accggacccg gctgacgcat gggccgcccc gacccctccg agctgctgac ccgcgtcatc
     1621 gagtcgatgg actccggcga ggaccgtccg cagcaacgcc agatggtcga ggccgtcgac
     1681 cgggcgttcc ggacccgcac gcacctggcg gtccaggggc cgaccggcgt cggcaagtcg
     1741 gtcgcctacc tgttgccggc gatcacatcg gcccggtccc atgccgacgc ccgcaccatc
     1801 gtggtcacct cgtccaaggc cctccaggac cagctcggcg aacgggacct gccgttcctg
     1861 gccgacgctc tcgacgtgcc gttccggttc gcggtgctca agggccgctc caactacctg
     1921 tgtcaggcgg cggtggccga gacccgggtc cagctcggtc gtggtgagga gcagcagacc
     1981 ctcgatctgg gcggcggctg ggacggggat gggtcggtcc cggaggttcg ggcccccgat
     2041 ccgatgctgc gagtcgagct cgaccgcctc ctcgagtggg ccggcaccac tcccacaggt
     2101 gagctggccg agctggcgga cccgccgtcg gatgcggcct ggtcggcgct gtcggtcgga
     2161 cccggtgagt gcgtcggagc gagccgctgc ggctacgccg cggagtgctt ctccgaacag
     2221 gcacgcgaac gggccggcgg ggccgacatc gtcgtggtca acgcccacct ctacgccgcg
     2281 gacgtccagg ccggcggcca gctcctgccc gcccacgacc acctggtcat cgacgaagcg
     2341 cacgagttcg aggacgccat ggtcggcgcg ctcggagtgt cgatgaccgg ttggcggatc
     2401 cgtcgactgg cccaggtcca cgaccgctgc gtggccgacg ccccgaccgt cgccatggcg
     2461 atcggcgcgg ccgccgacgc cgtcgacgac gccctggagc aggtcttcgg cgaggtgtcg
     2521 agcgaccgtg ggtccggccg gctcaccggc gagctgcccg acgtgatcgc cgacgcgctc
     2581 acccgagccg atctggccgt cgaccgggcc gtcaccagcc tgcgcgaggt ccacaaggct
     2641 gcgggcaacg ccccgatgag cgcggctgcg caccgcttcg agcgggccat ccgcaactgc
     2701 gacggcgtca gcgacgacct ccacgccctg cgcgacgacc tgcaccccgg ccaggtgcgg
     2761 tggatctcgg agggccgtcg gggacggttc gccctgaacc tcacccggat cgacatcggg
     2821 cccacgctgc ggaccaccgc ctgggagcgg ccggtagacg acgactcccc cgacgcggcg
     2881 accgacgacg gcgacggctc ggggggcccg accgtcgtca tgtgctcggc caccctcgat
     2941 cccggcaccg ccggccgcct cggcctgcgg gcggagtacc tggcggtcga ctccccgttc
     3001 gacttccgcc gccacggact gctctacgtg ccgcgcctgc cccggcccaa cgccgaggcc
     3061 tggcccgacg ccgtcgtcga cgagctcgtg cacgtgatcg agcgctgcgg cgggcgcacc
     3121 ctggccctgt tcacgtcgca ccgcatgctg cgccgcagcg tcgatgccgt ccgcgaccgc
     3181 ctgcccgacg cggacgtcct ggcccagggc gacgccccca accgcgtgct gcaggagcgc
     3241 ttcctggcaa acgagcacgc gtcgctgttc gcgaccgcca gcttctggac cggtatctcg
     3301 tcgccgggca cgacctgctc ggcggtggtg gtcgacaaga tcccgttccc ggtgccgacc
     3361 gatccgatcg tcgaggcccg gtgcgacgcg gtcggtgccg accgtgcctt catggaggtg
     3421 tcggtgcctg ccgcgggcgt gcagctcgcc cagggcgtcg gccggctcat ccgcaccgcg
     3481 accgaccggg gcgtggtcgc cgtgctcgac ccccgcctgg ccgaggcccg ctaccgggcc
     3541 cgcatcctcg accggctgcc ccggatgaag cgcacgcggg agcgcggcga cctcgacgag
     3601 ttcatcgact cgctcgggtt gggctgatca gtccgacccg gctcgctcca gcagccagtc
     3661 cgacggatcc ggcgacgaca gcaaccggcg gtacacggtg gtgtggtccg tccagttgtt
     3721 ggtgatgcgg ccgtccgcgt tcttgtacca ggagtggcag ccctcggccc acacggttcg
     3781 gtccgccgcg gcctgcacct cggtgtcgaa gcgcgacgcg gcgccctcgg agacctcgac
     3841 tgagcgcagt ccgagcagca ccttctcctc gatcaggcga agggtgtagc cgacctgctg
     3901 ctcgatcatg aacaggatcg agttgtggcc caaattggtg ttcggaccgt agagcatgaa
     3961 caggttcggg aacccgggca cggccagccc catgtaggca cgggcgccgt cgacccacac
     4021 ctcgttcagg tcacggccgg agcggccggt gatcttcagc ggggacagga actccgtcga
     4081 gcggaacccg gtgccgaaga tcagggtgtc gaccggggtc cggcgaccgt cggcggtgag
     4141 caccgcgccg tcctcgatgc gctcgatccc cgacgtgacc acgtcgacgt cgggccgcag
     4201 caaggtgggg taccagtcgt ccgcgatcag gatgcgcttg cagccgaccg ggtagtccgg
     4261 caccagggcg gcctcgggga ggcgttcgga gaccatcggt tggaggccct tgcggaactg
     4321 gccctgcatc cagcgcccca ggcggctccc ggcacgcatc acgttgaagc gggcctcgaa
     4381 gcgccagtag atcgactcgc ggtaggccct ctgggctcct gggacgttct cgaagatcca
     4441 gcgctcccgg ggggtgaact cgcggtcgtt gcggggcccg acgtagttgg cgctgcgttg
     4501 gaacaacgtc gtatgcgccg ctgaggcggc gaccggaggc acgaactgga tggcgctcgc
     4561 cccgatgccc acgaccccca cccgatcctc ggagaggtcg tggtcgtggt cccagcgcgc
     4621 cgagtggaac acggtccccc cgccctcgac gaaggcgtcg agaccgggga tgtccgggac
     4681 gtacggtcgg ttgagctgcc ccaggcccga cacgacgacg tcggcgacca ccgttccggc
     4741 ggcggtgccc tccgacggtt cgccgtccga tgtggccgtc cgcagcgtcc acgtgccggt
     4801 cgcgtcgtcg tagtgggcct cggtgacctc ggttcccaga cgcaggtgtc cgctcaggtc
     4861 gaagcggtcg accagcgact cgaagtagcc gaggatctcg ggctgaccgg cgaagcgccg
     4921 ggtccagtcc cgcttggacg cgaacgagaa gctgtacagg tggctgggga cgtcgcaggc
     4981 cgcgcccggg taggtgttgt cacgccacgt cccaccgacg ccgtcggact tctcgtagat
     5041 ggtgaacgag tcgatcccgg catcgcggag gcggatggcc atgcacaacc ccgccgcgcc
     5101 ggcgccgatg atcgcgacgc gcggcgtcgg ggcgcccgcc gcgagtgccg cccggaccgc
     5161 ggcggcacgc tcacgctcgc cgcgaccgcc gtagccggtg gtcccgatcg gccggagcga
     5221 tccgaccgga gcgggagtcg tggatgctgc acgcgacatg agacctcctg ggcgcacccc
     5281 ccgtgcgcgc ggccgacagt agtcccggcc cggcactgga ccggggcgtg gcgaccggcc
     5341 ggcctgctgg cggtcctctc agcgacagcc gggcgccacc gtcggtcggc caggcgcggg
     5401 gaatggggca cgtctggcta cggttgtgcc ccacatggcc accaccgagc tcaccgacga
     5461 caccttcgag accaccgtga agggcgacgg catcgtcctg gtcgacttct gggcggcatg
     5521 gtgcggcccg tgccggagct tcgccccggt gttcgagtcg gcgtcggagg acaaccccga
     5581 catcaccttc gccaaggtcg acaccgaagc caaccagcag ctggccggcg cgctgcagat
     5641 catgtcgatc ccgacgctga tgatgttccg cgacgggatc ctgctcttct cgcagccggg
     5701 cgcccttccc cgcgctgcgc tcgacgacct catcagccag gcccgcgagc tggacatgga
     5761 ccaggtgcgc gccgagatcg acgaccgggc gtcctgagcg gctcgaccgc cgacgcgttg
     5821 ccgtccggga tcagggcatc gagcccgcga agcgatcgtc gcggaggtcc gcgatctcgt
     5881 cctcggtgga gcgacgcttc ttccacacca cgacgcccac cgcggcgagc agcccgacga
     5941 cgacgaggac cagcagggtc cgtcggcccc gacacgttcg ggcggccacc tccagctcag
     6001 cgaccggggc cgactcctgt tccctgtccc ctcccaggtg atcggccacc gaccgggccc
     6061 gctcggccgc gatcgccgca ccttcgacca ccgcggcggt ggtcccggcc agcgtggcga
     6121 catgacggag gtccgaggag atcttgcgcg ctctgctcat ggggccaacc tagcccgcag
     6181 gtcggctgct cggtccgacg tcggggaacc gggtgtcggg atcgctctcg acggcgctgc
     6241 gggggacgtc cccgatcgat cagtcgacgg tgatgcgggc gaagagctcc atcttgcgct
     6301 cctcgaaggt gtcgaagtcg atccgcttgg cctcgaagtc ggcgtggagc tcctcgacga
     6361 ggcgcagcag ctgatccgcg gacaggtcct cgccctcggg gagctcggcg acgtcggtga
     6421 cgggctcgtc gtcgatctcg ttctcgccgg tgaggcggcg ctcgcgcttg gcggcggcct
     6481 tggccttctt ggcccggtcc cgctggagct tggcgaagct ggttcggctg gcacccatga
     6541 tcgtcctcga ttcgtctcgt gcccgtggtg tgcgagcggc cgtcccgatc tcttccgagc
     6601 gctgcggggt tcccggacgg gaggcatcgt tcccggtcgc cgcctgcgca ccgatccgtc
     6661 gggatctgtg gtccgggacg cgcggcggtc ggcgtgggcc cagtggtggg acccggcgac
     6721 cgctccagtg ggttccggtg ctcggaccct cagaggatag gcgagccgag ggcccgactc
     6781 acagccaccg cgccccgtgg taggcatcgt cgtgtcgatc cagggtggtt cggagaccgg
     6841 agacagcagc agatgaccct cggtagcagc ctggtggtgt cgcggagtga cctcgatcac
     6901 atggaggtcc ggccgtcggt gctcacggac ggatcgacgc caccgacggg ttcggcgctg
     6961 ctggcgatcg agtcgttcgc cctgtcgacg cacacggtca cgaccgccgc ctcggctgac
     7021 agcaccgggg cgtggcgcca cttcccggcg cccgacggct tcgggcacat cccgacgttc
     7081 ggtcgggcgc gggtggctcg ctcggggcac ccggacctgg ccgaggggct cgaggtgacg
     7141 ggctacctgc ccatggccac ccacctgctg gtgacacctg agcggatcga tcagcggggc
     7201 ttctccgacg gcgcacccca ccggcgcgga tcggccgggg tccatgatcg gtacctgttc
     7261 cgtgcggtcg acccgacgtc ggtcgccgcg gatgatccgg acctcgaggt ggcgctgcgg
     7321 ccccagttca cgatggcgtt cctgatcgtc gcgggtctct cggcacgcag gatggacgag
     7381 gtggccgccg tcgtggtcac cagcgcctcc tgccgcaccg ccctcggcgt ggcgcacctc
     7441 ctgggggcgc tcgagcatcc gccggcggtc atcggtgtca cccgcgacga ccacgtggcc
     7501 gacacggccg cgtcggggtg ctacgaccgg gtgctgtcgt accgggacgt cgcgtccctg
     7561 ccggtcgggc ccacgatggt cgtcgacttc tgcggtgacg gtgcggtggc ccacgaaatc
     7621 caccaccacc tcggggactc gctgctccgg tcgatcgtgg tcggagccac ccaccggaat
     7681 gcagttcctg tcgacaccgc ggcgctccca ggggcgaccc gggagatctt cctggcccct
     7741 gaggtcgccg aggacctgcg tcggcgggtc gacgacgcgg cgatcgaggt ggccttcacc
     7801 ga
//
LOCUS       DAOQOD010000142         7764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3516_length_7764_cov_12.627708, whole genome shotgun sequence.
ACCESSION   DAOQOD010000142 DAOQOD010000000
VERSION     DAOQOD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3516_length_7764_cov_12.627708"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLZ72_12340"
     CDS             complement(<1..598)
                     /locus_tag="PLZ72_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNA37131.1"
                     /translation="MSDTITVGELLVRALRMEGVDFMCGIVDGAHIPFVRHLDAHGIR
                     YVNTRHEEAAAHIAEAYARLTHRPAVVIGNPGPGGANMLAGLTSAQGEGHPVVAIACT
                     RRASVNSPDRGGAWQATDLVDMARPITSYSALISRADRVPDMVRSAFRAATVGRPGPA
                     FLAIPDELLTQPVDLDAVALTPAGRHRVIDVGAGDPTRI"
     gene            708..1250
                     /gene="mobB"
                     /locus_tag="PLZ72_12345"
     CDS             708..1250
                     /gene="mobB"
                     /locus_tag="PLZ72_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNA37132.1"
                     /translation="MSVRPLVVSFVGPSGVGKTTVIESLVAALAARGLRVGTAKHAPH
                     GFQVDREGSDSWRHRAAGAERVLLMGSASAVVFLGAPEDGSGGDHHRPSSPVDADRMV
                     ELLDAQMSSMDVVLVEGFAPFSDRVVVVERSTVEPKVDLVPVDVWLTVSDRGDGGDGS
                     FGFDELDRLVVRLVDERNSA"
     gene            1365..4886
                     /locus_tag="PLZ72_12350"
     CDS             1365..4886
                     /locus_tag="PLZ72_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA37133.1"
                     /translation="MVDSDGVTPHSAQPRASVPRRALLAAGPGAVVAALASPHGRAAL
                     APHLSMLQSIEAIPNPLAAYPNRDWEDVYRDLYRPDSTYHYLCAPNDTHGCLLKASVK
                     NGVAVYADPSFGYHRARDIYGNTASSRWDPRACVSGLAYVRRAYSDRRVKGCYVRKGF
                     RDWVDAGMPRGDDGQPPLKYRSGRGKESFVKVTHEEAADLAARVYTDVARTYSGPDGA
                     RRLEEQGYYEPEMIEAMHGAGTQTLKFRGGMPCNAPFRIGGFYRLANMMALLDVAVRD
                     VDPSESYGGRHWDSFSWHTDLPPGHPMVTGQQAMDFDLNTAENAGLITLWGMNWIATK
                     MPDGHWLTEARLHGAKVVTIAPEYQSSSCKADRAITIRAGADSALAFGLAHVIVRDRL
                     YDTDFVTRHTDLPLLVRTDTKKLLQARDLIAGYTSAPLRTVRLVEPGVPLPPPAEQPQ
                     QFVPADLREEWGDQVVWNTRTGAPAVVTHDMTGINLPPEVEPALEGTFTVTLADGSSV
                     EVRPVFDAVAQYLRDTCSPEQISKVTWVPVDAIEELAADIAANSGRTLFVTGMGPNHF
                     FNNDNKDRGVLLVAALTDNIGHYGGTVGSFSGNYRLPNFSGIAQWSYEDPFAASLDPA
                     AKAKVNKRLKAESAHFYNYGDRPLKVGKKMFTGHTHMPTPTKTMHFANSNSLLGNAKG
                     AHDVFVNTLPKIEMIIHHEWYWTASCEYADIVFGVDSWPERQLPDVYSSMTNPFLQAW
                     PSTPMERIHPTLDDMECNALVAAALSEQTGDPRFADYWRFVAEGRPGVYIQRVFDAGN
                     ATRGYSFDALHESCRNGTPFLTLMRTYPRIPGWEQTNESKPWYTKSGRLEFYRDEDEF
                     IEYGENLPLHREPVDGTVYEPGVIMAAPHPFIDPVQPSDYGLDPDDLSTEVRQVRHVV
                     RTPEEIVSSEHPLRRDGFSHVLVTPKFRHACHSMGASTDADVLIFGPFGDFYRHDTRK
                     PWMSEGYVDLHPDDAEELGVSDGDYVWVDADPADRPFKGWQDNPDDYHVFRWMVRARV
                     NPSVVRKVARAWFHFHVATHGSVQGHEERDDGLARNPRTDYQAGYRYGSHQSVTRAWL
                     RPTLMTDSLTRKEGAGQLVGQGFELDVYGAVGAPKESFVKFTKAEDGGEDGEGSWYPA
                     EQGYRPGSEKRSMQRYLEGGFITTGKG"
     gene            4890..6029
                     /locus_tag="PLZ72_12355"
     CDS             4890..6029
                     /locus_tag="PLZ72_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNA37134.1"
                     /translation="MAEVFNWQINRSMTYPYEEARPQRQAAAVFDTNKCIGCQTCTMA
                     CKTCWTSGHGQEYMLWNNVETKPWGSYPLAWDVRELQLLGPQSWDGGTYTGQTIFEAA
                     PQGERNVAWKPTEMDYAYPNRGEDETNVSLTGKRYHLKFPHDMWFFYLPRICNHCTYP
                     ACLQACPRQSIYKRPEDGIVLVDEERCRGYRVCFEACPYKKIFYNHTTKVSEKCVMCF
                     PAVEQGIQPRCMRNCIGKIRSFGYLSRPEEAREDNPIDFLVHVRKVGKPLYPQFGLEP
                     NVYYIPPIHVSSNAFLTMLFGPGTRGAIEAYRAAMNGEDRELLAALLLAVSTDRIITR
                     FELVGDRVDAWAENGDQVVSMPLRVPTVERAPFDPELDVYRYNFT"
     gene            6039..6953
                     /locus_tag="PLZ72_12360"
     CDS             6039..6953
                     /locus_tag="PLZ72_12360"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNA37135.1"
                     /translation="MALMQLRTSRRTVLKGAAVGAGAAALASCSAGSYGGRGVLSRRV
                     SEVPLDPTDATWDDTEYTDVVLGPQNVALPQRLDPAVTAVRVRSVHDGRQVGFRLEWT
                     DEKPRDQTIRVDDYRDACAVLLGPGDADANLRMMGTAEIPVTLLHWKADWQRMLADGD
                     EGLDAAFPNRTVDTYPVVHRVPPEEVGIESYVEADATEWLPAIHVGNPVAGRAVRTTS
                     VEKAIAAGFSTTTSAGDQDATGRGERTDEGWQVVIVKPLTPTEEGEIDLAPGTVSTCA
                     FAVWSGSDRDAGSRKCPATEVAALELEG"
     gene            6950..7693
                     /locus_tag="PLZ72_12365"
     CDS             6950..7693
                     /locus_tag="PLZ72_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNA37136.1"
                     /translation="MTVALAASVDDARRLRSAAVAAVLLARSLGPDTSALVDGETRAA
                     LGDALAGAGADAALARLVDRDSRSVEADQGGLDATELAGRWVRWFDLGRVAPYEGSNV
                     ASTAAGVTPRLADIAGFYRALGLQVVDDRPDHVVAQLEFLAMALMAEAEATEAGDSES
                     AARTSDLIRKFLRDHLGGWIDTWASRVAAVDELAPWVPAAAAAAELVRAESVRRNVIP
                     LRDAAVLPADAGVPEDDEALLACDGTEPD"
BASE COUNT         1202 a         2693 c         2721 g         1148 t
ORIGIN      
        1 cgatccgggt cgggtcgccg gcgccgacgt cgatcacccg gtggcgtccg gccggggtga
       61 gagcgacggc gtcgaggtcg acgggctggg tgagcagctc gtcggggatg gccaggaacg
      121 ccggtccggg ccgaccgacc gtcgcagcgc ggaacgcgga gcgcaccatg tcggggacgc
      181 ggtcggcgcg ggagatcaac gccgagtagc tggtgatcgg gcgggccatg tcgaccaggt
      241 cggtcgcctg ccacgccccg ccgcggtcgg gcgagttgac cgacgcccgg cgggtgcagg
      301 cgatggcgac gaccgggtgg ccctcgccct gggcgctggt cagcccggcc agcatgttgg
      361 cgccgcccgg tccggggttg ccgatcacca ccgcgggtcg gtgcgtcagt cgggcgtagg
      421 cctcggcgat gtgggcggcg gcctcctcgt ggcgggtgtt gacgtagcgg atcccgtgcg
      481 cgtcgaggtg gcgcacgaac gggatgtggg cgccgtcgac gatgccgcac atgaagtcga
      541 cgccctccat ccgcagcgcc cggaccagca gctcgccgac ggtgatggtg tcgctcatcg
      601 gctcatcatg acggccgctc cgatcacggt ccacgggctg tgcgccaggt cacagggccg
      661 gcggccgccg gcgtgtcggc gtgtgcatcc cggtagcgtc agctccgatg tcggtccgcc
      721 ctctcgtcgt ctcgttcgtc ggcccgtcgg gtgtgggcaa gacgacggtg atcgagtcgc
      781 tcgtcgctgc cctcgcggcg cgcgggttga gggtcggcac ggccaagcac gctccgcacg
      841 ggttccaggt ggaccgtgag gggtcggact cctggcgcca tcgggccgcc ggtgccgagc
      901 gggtcctgtt gatgggatcg gcgagtgccg tggtcttcct cggtgccccc gaggacggct
      961 ccggcggtga ccaccatcgg cccagctcgc cggtcgacgc ggatcgcatg gtcgagctgc
     1021 tcgacgcgca gatgtcgtcg atggacgtcg tgctggtcga ggggttcgcc ccgttctcgg
     1081 accgggtcgt cgtcgtcgag cggtcgacgg tcgagcccaa ggtcgacctc gtccccgtcg
     1141 acgtctggct gaccgtctcg gatcggggcg acggcggcga cggctccttc ggcttcgacg
     1201 agctcgaccg tctcgtcgtt cgcctcgtgg acgagcgcaa ctcggcctga ggtccccgga
     1261 tccgggcacc gtcgccgcgg aaccgacgtg cgacaggagt cacggggcga atcgggttcg
     1321 acctgtctta gggtccgaac aagagccgag tctcgggagg tcccatggtg gattccgacg
     1381 gtgtgacgcc acactccgct cagccccgcg cgtcggtgcc gcgccgggct ctgctggccg
     1441 ccggtcccgg cgcggtggtg gcggccctgg cgagtccgca cggtcgagcg gcgctcgccc
     1501 cccacctctc gatgctccag tcgatcgagg ccatccccaa cccgctggcc gcgtacccga
     1561 accgggactg ggaggacgtc taccgcgacc tgtaccggcc ggactcgacc taccactacc
     1621 tgtgcgcgcc caacgacacc cacgggtgcc tgctgaaggc gagcgtcaag aacggcgtcg
     1681 cggtctacgc ggatccgtcc ttcgggtacc accgggcgag ggacatctac gggaacaccg
     1741 cgagcagccg ctgggacccg cgggcgtgcg tgtcgggact ggcgtacgtc cgtcgggcct
     1801 acagcgaccg ccgggtcaag ggctgctacg tgcgcaaggg gttccgggac tgggtcgacg
     1861 cgggcatgcc gcgcggtgac gacggccagc ccccgctgaa gtaccggtcc ggtcgtggca
     1921 aggagtcctt cgtcaaggtg acccacgagg aggctgccga cctggccgcc cgggtctaca
     1981 cggatgtggc ccgcacctac agcggtcccg acggtgcccg tcggctcgag gagcagggct
     2041 actacgagcc cgagatgatc gaggccatgc acggggcggg tacccagacc ctcaagttcc
     2101 gtggcggcat gccctgcaac gcgccgttcc ggatcggcgg cttctaccgg ctggccaaca
     2161 tgatggcgct gctcgacgtg gcggtgcgcg acgtggatcc ctcggagtcc tacggcgggc
     2221 ggcactggga cagcttctcg tggcacaccg acctgccccc cggccatccg atggtcaccg
     2281 gccaacaggc catggacttc gacctcaaca ccgccgagaa cgccggtctc atcacgctgt
     2341 ggggcatgaa ctggatcgcc accaagatgc ccgacgggca ctggctcacc gaggcccgac
     2401 tgcacggggc caaggtcgtg acgatcgcgc cggagtacca gtcgtcgtcg tgcaaggccg
     2461 accgggccat caccatcaga gcgggcgccg acagcgccct ggcgttcggg ttggcgcacg
     2521 tgatcgtgcg cgaccgcctg tacgacaccg acttcgtcac ccgccacacc gacctcccgc
     2581 tgctggtgcg caccgacacc aagaagctcc tccaggcgcg ggacctgatc gccggctaca
     2641 cgtcggctcc gctgcgcacg gtgcgactgg tcgagcccgg cgtgccgctg ccgcctccgg
     2701 ccgagcagcc ccagcagttc gttccggccg atctgcgtga ggagtggggc gaccaggtgg
     2761 tgtggaacac ccggaccggc gcgcccgcgg tggtgaccca cgacatgaca ggcatcaacc
     2821 tgccccccga ggtcgaaccg gcgttggagg ggaccttcac cgtgacgctg gccgacgggt
     2881 cgagcgtcga ggtccggccg gtgttcgacg ccgtcgcgca gtacctgcgc gacacctgct
     2941 cacccgagca gatctcgaag gtcacgtggg tcccggtcga cgcgatcgag gagctcgccg
     3001 ccgacatcgc ggcgaactcg ggccgcaccc tgttcgtcac gggcatggga cccaaccact
     3061 tcttcaacaa cgacaacaag gaccggggcg tcctgctggt cgcggcgctc accgacaaca
     3121 tcggccacta cggcgggacg gtcggatcct tctccggcaa ctaccggctg ccgaacttct
     3181 ccggcatcgc ccagtggagc tacgaggacc ccttcgcggc ctcgttggac ccggccgcca
     3241 aggccaaggt caacaagcgg ctcaaggcgg agtcggccca cttctacaac tacggcgacc
     3301 gtccgctgaa ggtgggcaag aagatgttca ccgggcacac gcacatgccc acgccgacca
     3361 agacgatgca cttcgcgaac tcgaactcgt tgctgggcaa cgccaagggc gcgcacgacg
     3421 tgttcgtcaa cacgctgccc aagatcgaga tgatcatcca ccacgagtgg tactggaccg
     3481 cgtcctgtga gtacgccgac atcgtcttcg gggtcgactc ctggcccgag cgtcagcttc
     3541 ccgacgtcta cagctcgatg accaacccgt tcctccaggc ctggccgtcg accccgatgg
     3601 agcggatcca ccccaccctc gacgacatgg agtgcaacgc cctggtggcc gcggcgctca
     3661 gcgagcagac cggcgatccc cgcttcgccg actactggcg attcgtcgcc gagggccgac
     3721 cgggcgtcta catccagcgc gtcttcgacg ccggcaacgc cacacgcggc tactccttcg
     3781 acgcgctgca cgagtcgtgc cgcaacggga cgccgttcct gacgctgatg cggacctacc
     3841 cgcgcatccc gggctgggag cagaccaacg agtccaagcc ctggtacacc aagtcgggcc
     3901 gcctcgagtt ctaccgggac gaggacgagt tcatcgagta cggcgagaac ctcccgctgc
     3961 accgtgagcc ggtcgacgga acggtgtacg agccgggcgt gatcatggcc gccccgcacc
     4021 cgtttatcga tccggtgcag ccgtcggact acgggctcga ccccgacgac ctctcgaccg
     4081 aggtccgcca ggtacgccac gtcgtccgca cccccgagga gatcgtcagt tccgagcacc
     4141 ctctgcgccg tgacggcttc agccacgtgc tcgtcacccc gaagttccgc cacgcctgtc
     4201 actccatggg cgcctcgacg gacgctgacg tgctgatctt cggccccttc ggggacttct
     4261 accgccacga cacgcgcaag ccgtggatgt cggagggcta cgtcgacctc caccccgacg
     4321 acgccgagga gctcggcgtc tccgacggcg actacgtgtg ggttgacgcc gatccggccg
     4381 accgcccgtt caagggctgg caggacaacc ccgacgacta ccacgtgttc cgctggatgg
     4441 tgcgggcccg ggtcaacccg tcggtggtgc gcaaggtggc ccgggcctgg ttccacttcc
     4501 acgtcgccac ccacgggtcg gtgcagggcc acgaggagcg cgacgacggc ctggcccgca
     4561 acccccggac cgactaccag gcgggctacc gctacggcag ccaccagtcg gtgacgcggg
     4621 cgtggctcag gccgacgctc atgaccgact cgctcacccg caaggagggc gccggtcagc
     4681 tcgtcggcca ggggttcgag ctcgacgtct acggagcggt cggagctccc aaggagtcgt
     4741 tcgtgaagtt caccaaggcc gaggacggcg gagaggacgg cgagggttcc tggtacccgg
     4801 ccgaacaggg ctaccgcccg ggcagcgaga agcgctcgat gcagcggtac ctcgagggcg
     4861 gcttcatcac gacagggaaa ggctgagcca tggcagaggt gttcaactgg cagatcaacc
     4921 ggtcgatgac ctacccctac gaggaggcac gaccgcaacg ccaggccgcc gcggtgttcg
     4981 acaccaacaa gtgcatcggg tgccagacct gcacgatggc gtgcaagacg tgctggacct
     5041 cggggcacgg ccaggagtac atgctctgga acaacgtcga gaccaagccg tggggctcgt
     5101 accccctggc gtgggacgtg cgggagctcc agctcctggg accgcagagc tgggacggcg
     5161 gcacctacac cggccagacg atcttcgagg ccgcgccgca gggcgagcgc aacgtcgcct
     5221 ggaagccgac cgagatggac tacgcctacc cgaaccgggg tgaggacgag accaacgtgt
     5281 cgctgaccgg caagcggtac cacctgaagt tcccccacga catgtggttc ttctacctgc
     5341 ctcgcatctg caaccactgc acctatccgg cctgcctcca ggcgtgcccc cgccagtcga
     5401 tctacaagcg ccccgaggac ggcatcgtgc tggtcgacga ggagcgctgc cgcggctacc
     5461 gggtctgctt cgaggcctgc ccctacaaga agatcttcta caaccacacc acgaaggtgt
     5521 cggagaagtg cgtgatgtgc ttcccggcgg tggaacaggg catccagccg cgctgcatgc
     5581 gcaactgcat cggcaagatc cgttcgttcg gctacctgag ccggccggag gaggcccggg
     5641 aggacaaccc gatcgacttc ctggtgcacg tgcgcaaggt cggcaagccg ctgtacccgc
     5701 agttcggcct ggagccgaac gtgtactaca tcccgccgat ccacgtctcg agcaacgcct
     5761 tcctgacgat gctgttcgga ccaggaaccc gtggcgccat cgaggcctac cgggccgcca
     5821 tgaacggcga ggaccgggag ctgctcgcgg cgctgctgct ggcggtgtcc accgaccgga
     5881 tcatcacccg cttcgagctc gtcggtgacc gggtcgacgc ctgggccgag aacggcgacc
     5941 aggtggtcag catgccgctg cgggtcccga cggtggagag ggcgcccttc gatcccgagc
     6001 tcgacgtcta ccgctacaac ttcacctgag gagacgagat ggctctcatg cagctgcgga
     6061 cctcacggcg gacggtgctg aagggagcgg ccgtcggggc cggcgccgcg gcgctggcct
     6121 cgtgcagcgc cggttcgtac ggtggtcggg gagtcctgtc ccgacgggtg tcggaggtgc
     6181 ccctcgaccc gacggacgcg acctgggacg acaccgagta caccgatgtc gtactcgggc
     6241 cgcagaacgt agcgctgccc caacggctgg atccggcggt gaccgcggtt cgggtccgat
     6301 cggttcacga cggccggcag gtcgggttcc gattggagtg gaccgacgaa aagccccggg
     6361 accagacgat ccgggtggac gactaccgcg acgcgtgcgc ggttctgctc ggcccgggtg
     6421 acgcggatgc gaacctgcgc atgatgggca ccgccgagat cccggtcacg ctcctgcact
     6481 ggaaggcgga ctggcagcgg atgctcgcgg atggcgatga ggggctcgac gccgcgttcc
     6541 ccaaccggac ggtcgacacc tatcccgtgg tccaccgggt gccgcccgag gaggtgggca
     6601 tcgagtccta cgtcgaggcc gacgccaccg agtggctccc ggcgatacac gtcgggaacc
     6661 ccgtcgccgg tcgggcggtc cgaaccacct cggtggagaa ggcgatcgct gccgggttct
     6721 ccaccacgac ctcggccggc gaccaggacg cgaccggtag gggggagcgc accgacgagg
     6781 gctggcaggt ggtcatcgtg aagccgttga ccccgaccga ggagggtgag atcgacctcg
     6841 cgcccgggac ggtgtcgacc tgcgctttcg ccgtctggtc cggctccgac cgcgacgccg
     6901 ggtccaggaa gtgtcccgcc accgaagtcg cagcactcga gttggaggga tgaccgtggc
     6961 gctggcggca tcggtggacg acgcccgcag gctccgctcg gcggcggtcg ccgcagtgct
     7021 gttggcccgg agcctggggc ccgacacctc ggccctggtc gatggcgaga cccgtgcagc
     7081 gctcggtgac gctctggccg gcgccggggc tgacgccgcc ctggctcggc tggtggaccg
     7141 cgactcgcga tcggtcgagg ctgatcaggg cggtctggat gcgaccgagc tcgcgggtcg
     7201 ttgggtgcgg tggttcgatc tgggacgggt cgctccctac gagggttcga acgtggcgtc
     7261 gaccgcagcg ggtgtgacgc cccgcctggc cgacatcgcc ggcttctacc gggccctcgg
     7321 gctccaggtc gtcgacgatc ggcccgacca cgtcgtggcc cagctcgagt tcctggccat
     7381 ggccctgatg gccgaggccg aggcgaccga ggccggagac agcgagtccg cggctcggac
     7441 ctcggatctg atccggaagt tcctccggga ccacctcggc ggctggatcg acacctgggc
     7501 gtcgcgtgtg gcggcggtcg acgagctcgc gccgtgggtc cctgcggcgg ccgccgcggc
     7561 ggagctcgtc cgggctgagt cggtgcgacg gaacgtgatc ccgctccgcg acgcggcggt
     7621 gctccccgcg gatgccggag tgcctgagga tgacgaggcg ctgctcgcct gcgacggcac
     7681 agaaccggac tgaccgcggc ggaccccgcg cgttgttgta gtcctcggtc gctgtggcga
     7741 cctgtcggct acaagttcgg gggc
//
LOCUS       DAOQOD010000143         7722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3553_length_7722_cov_11.097952, whole genome shotgun sequence.
ACCESSION   DAOQOD010000143 DAOQOD010000000
VERSION     DAOQOD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_7722_cov_11.097952"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLZ72_12370"
     CDS             complement(<1..686)
                     /locus_tag="PLZ72_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNA37137.1"
                     /translation="MGIEREMKFDLEAALEPDLEGLGAAVGARRSVELVADYWDTRSG
                     HLRAWGITVRHRRSDDGTEDGWTVKIPVPQTRASSPPGRRNGTERTRVEIDVAGGPDV
                     PPSRVLAIVSGLACSEPFERVAHLVTDRCSTILVEESSGGGQSCDSDAPEDFPGAVRV
                     DRDHVTAFTADGVAEFDQLEVEWAGSDELWDRVTDWAHRSGLRPTAATNKLEQALGPG
                     RIRAPKVPHLG"
     gene            803..1975
                     /locus_tag="PLZ72_12375"
     CDS             803..1975
                     /locus_tag="PLZ72_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HNA37138.1"
                     /translation="MGRHHVRNATVAVLGTVLVVAAACTPTPTPPTGPVDPAPPEIHD
                     VRLVTPRTEAPVTATLRWRISDPNGDALTCRIDLDGDGTHDRTISPCPTAGDLLVTYR
                     TAGTVVASLDVSDGTFDAPTWTTDPLFVTAGPSEPFDITLHFDPATAPEYRDAFAAAS
                     ARWQQVVVDGWNPEPLVVPAGFMGWIPAFDGAVDDVLIAARVVELDGPGGLLGQAGAL
                     AKRASSGEPYFGLMEFDAADLADYAAAGELVDLIVHEMGHVLGIGFNWVGEGRVDDLL
                     TNPTYNGPAANAAWHELGGTGQVPVEDQGDVGTRAAHWRESTFANELMTGYADSDERL
                     SRVTIGALADRGYGVELSAADDYTLPAPWAGAARRAGRAPQHTTPVALLPEGMLKG"
     gene            2002..2973
                     /locus_tag="PLZ72_12380"
     CDS             2002..2973
                     /locus_tag="PLZ72_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNA37139.1"
                     /translation="MARSATKVPPADVELRYRTVHGYQRAYRMAGSGPALLLIHGISD
                     DSESWLPVMGRLAEHHTVIAPDLLGHGASDKPRADYSVAAFSNGMRDLLDVLDIERVT
                     VVGHSLGGGVAAQLVYQYPERVERLALVSAGGVGREVSPVLRAAAAPLSEVGMLPLMV
                     PGANEVIGAVLGVLGRLGLDIGRDAGEVARVLRGMPDRRQRVAFTRTLRAVVDRHGQL
                     VTMLDRAYLAQAMPVMLVWGDHDGIIPVEHAHRAHAAMPDSRLSIYPGAGHFPHHADP
                     ERFVDELLDFIATTAPYRSDTESRRQMLLRGPGGVALDEPDEPALLG"
     gene            3012..3521
                     /locus_tag="PLZ72_12385"
     CDS             3012..3521
                     /locus_tag="PLZ72_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37140.1"
                     /translation="MARRSTTSSILAAGGRLPVLVLRRDPTDSVLIEQHLERSNRLDV
                     QVVRPADLVRTPLRWPADPRIVPLAVCVLFRDRPGSIAAASRLVEHLGDVPVVGVLDA
                     PYPDEPTAEMAAELVTAGVADVIDIDHLSAHVLEQVLLGAIDRSVAGVVHLGDSVSVL
                     DDEHRRAAR"
     gene            complement(3541..4158)
                     /locus_tag="PLZ72_12390"
     CDS             complement(3541..4158)
                     /locus_tag="PLZ72_12390"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA37141.1"
                     /translation="MDEPGLREKKKRQTRRRIEDAATELFARDGFDATTIEGIARHAE
                     VAPRTFFHYFETKEDVVLADYADRLRLLLERLSARPAGEEPWESLRQAFRAVASDDSG
                     HRDAMSKRLRMMAEAPTVAARSLLLQSMWEHDLAELLSSRVQDADHESPSPELLAAAA
                     LAAMRASIQQWLADPSIDLPTRVDLCFELMGKGLGSGHRRRSTGT"
     gene            4278..6437
                     /locus_tag="PLZ72_12395"
     CDS             4278..6437
                     /locus_tag="PLZ72_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNA37142.1"
                     /translation="MTNWLYRIGRGSAGRRWLVVFGWVLLLATLAITNRAVGGQPVDS
                     LEVPGVESQQAVDLLRQRFPERAGATAMVVFHTQTTTIDSRSAADAVSATVADLQTLP
                     HVISVTDPLTTPGAIAPDRSTAVAALQYDRSTADLGHAALDALVASGTTAERAGVQVE
                     FGGELPTVLKKESTGPAEAIGVVAALVILFVTFRTVASMALPLLTAATGLLAGLSAVG
                     LLSGLIEVPTVAPRLGTMIGLGVGIDYALFVLSRHRDNLASGMPIDESIGRTNATAGK
                     AVVIAGATVVVAILGLQFAGIPFVAALGYSAAIVVTFAVVVAITLLPALLALVGERVL
                     PRSWRASHRPVVEQAAPTGEGPTATRWVRWASWVTHRPWRSAIAATAVLLIAAVPLID
                     MQLGQADAGTQPADTTQRRAYDLLAAGYGAGFNGPLLLVVDLGRSPDLQVVTLIREAV
                     ATDPGIGFVSPPTTNSAQDTVVLTAVPTTEPQARATSELVHRLRGNTLPSAVGDSGAK
                     VLVGGPTASFIDQSDRIAERLPRFISAVVAMSFLLLMIVFRSILVPLKAALLNLLSIG
                     AAYGVVVAVFQWGWGRSLIGLDEAVPIASFVPMMMFAILFGLSMDYEVFILSRIREEH
                     LAGRSNNDSVVTGLGATAKVITAAALIMVSVFVGFVMSPDPIVKMMGIGLATAVAIDA
                     TVVRMVLLPATMALVGEPNWWLPRWLDRVLPHLDVEG"
     gene            6571..7722
                     /locus_tag="PLZ72_12400"
     CDS             6571..7722
                     /locus_tag="PLZ72_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37143.1"
                     /translation="MLQLTEEHHLFRQTVREVVTNEIDAHADEWERDGDFPAHDLFRR
                     FGELGLLGLEYDPAYGGGGADHTYTMILGEELGRSASLGVSMALSVQTDMATPALHRF
                     GTAELKERYLRPAIAGEHVAAIGVSEPDAGSDVAGVRTRAVRDGDEWVINGTKLWITN
                     GTQADWICLLCRTSDEGGYRGMSQVIVPTDTPGFSVSRKLDKLGMRASDTAELSFDDV
                     RVPVANTIGEAGRGFQQQMAQFQNERMIAAYQMVGATELALERTVAYLRERQAFGAPL
                     MANQALQYELADLAADLDLVRHHNWAAVAAYLNGEDITRQATVAKLKAARLARRMADL
                     AVQFHGGMGYVEENWPARFFRDTRLWSIGGGADEVMLRTLARMNGIVGD"
BASE COUNT         1081 a         2786 c         2641 g         1214 t
ORIGIN      
        1 ccgaggtggg ggaccttcgg tgctcggatc cgacccggtc cgagcgcctg ctccagcttg
       61 ttcgtcgcag cggtgggccg caggccggag cgatgggccc agtcggtgac gcggtcccac
      121 agctcgtcgc tacctgccca ctccacctcg agctggtcga actcggcgac gccgtcggcg
      181 gtgaacgccg tcacgtggtc gcggtcgacc cgcacggcac cggggaagtc ctccggagcg
      241 tcgctgtcgc agctctgccc gccgccactg ctctcctcga cgaggatagt cgaacaccgg
      301 tcggtcacca ggtgcgccac tcgctcgaag ggctcggagc acgccagccc cgacacgatg
      361 gccagcacgc gcgacggggg cacgtcggga ccgccggcga cgtcgatctc cacccgggtc
      421 cgctcggtgc cgttccgtcg tccgggcggg gacgacgccc gcgtctgcgg gacggggatc
      481 ttcaccgtcc agccgtcctc ggtgccgtcg tcggagcgtc ggtggcgcac ggtgatcccc
      541 catgcccgga ggtggccgga gcgggtgtcc cagtagtcgg cgaccaactc gacggaccgg
      601 cgcgctccga ccgctgcgcc gaggccctcg aggtccggct cgagggccgc ctccaggtcg
      661 aacttcatct ccctctcgat tcccatgccc ctccctaccc caatcgacac cgtcggtgcg
      721 gtccggagca cgttccaccg gcgcttcccg gcctaccatc gggggtccga accgatgggg
      781 atgttcggac cgggagggaa ccatgggccg tcaccacgtt cgcaacgcca ccgtcgccgt
      841 gctcgggacg gtgctcgtcg tggccgcggc atgcacgccg actccgacgc cgccgaccgg
      901 cccggtcgac cccgccccgc ccgagatcca cgacgtccgc ctggtgacac cccggaccga
      961 ggcaccggtc accgccacgt tgcggtggcg catcagcgac cccaacgggg acgctctgac
     1021 gtgtcggatc gacctcgacg gcgacggcac ccacgatcgc accatctctc cgtgcccgac
     1081 agccggcgac ctcctcgtca cctaccggac cgccggcacc gtcgtcgcgt cgctcgacgt
     1141 gtccgacggc accttcgacg ccccgacctg gaccaccgat ccgctgttcg tgaccgcggg
     1201 cccgtccgaa ccgttcgaca tcaccctcca cttcgacccg gccaccgccc ccgagtaccg
     1261 cgatgccttc gccgcggcgt cggcccgctg gcaacaggtg gtggtcgacg gctggaaccc
     1321 cgagccgctg gtcgtccccg ccggcttcat ggggtggatc ccggcgttcg acggggccgt
     1381 cgacgacgtg ctgatcgcgg ctcgggtcgt cgagctcgac gggccgggcg gtctcctcgg
     1441 ccaggccggc gcgctcgcca agcgggcgag cagcggcgag ccctacttcg ggctgatgga
     1501 gttcgacgcc gccgacctcg ccgactacgc ggcggccggt gagctcgtcg acctgatcgt
     1561 ccacgagatg ggccacgtgc tcgggatcgg cttcaactgg gtcggcgagg gccgcgtcga
     1621 cgacctgctc accaacccca cctacaacgg acccgccgcc aacgcggcgt ggcacgagct
     1681 gggcgggacc ggccaggtcc ccgtcgagga ccagggcgac gtcgggaccc gggcggccca
     1741 ctggcgggag tcgacgttcg ccaacgagct catgaccggc tacgccgaca gcgacgagcg
     1801 cctcagtcgg gtcacgatcg gcgccctggc cgatcggggc tacggcgtcg agctgtccgc
     1861 ggccgacgac tacacactgc ccgccccctg ggccggcgcg gcccgccggg ccggccgtgc
     1921 accgcagcac acgaccccgg tggctctgct gccggagggg atgttgaagg gctgaccgcc
     1981 tccacgggta cgctccgcgt cgtggcccga tctgcgacca aggtcccccc ggccgatgtc
     2041 gagctccgct accggacggt ccacggctac cagcgggcgt accgcatggc cggctcgggt
     2101 ccggcgctgc tgttgatcca cggcatcagt gacgactccg agagctggct gccggtcatg
     2161 ggtcgtctgg ccgagcacca caccgtcatc gcccccgatc tgctgggtca tggcgcatcg
     2221 gacaagcccc gcgccgacta ctcggtcgcc gcgttctcga acgggatgcg ggacctcctc
     2281 gatgtcctcg acatcgagag ggtcaccgtc gtcggtcact cgctcggcgg gggtgtggcc
     2341 gcccagctcg tctaccagta cccggagagg gtggagcgcc tggcgttggt cagcgccggc
     2401 ggtgtgggac gtgaggtcag tccggttctg cgggccgcgg ccgccccgct gtccgaggtc
     2461 gggatgctgc cgctgatggt gccgggcgcc aacgaggtga tcggagcggt gctcggagtg
     2521 ctcggtcggc tcggcctcga catcggtcgc gacgccggcg aggtcgcccg ggtgttgagg
     2581 ggcatgcccg accggcgtca gcgtgtggcg ttcacccgta cgctccgtgc tgtggtcgac
     2641 cgtcacggac agctcgtgac catgctcgac cgggcctacc tggcccaggc gatgccggtc
     2701 atgctggtct ggggcgacca cgacgggatc atccctgtcg agcacgccca ccgggcccat
     2761 gcggccatgc ccgacagccg cctgtcgatc tacccgggcg ccggtcactt cccgcaccac
     2821 gcggacccgg agcgcttcgt cgacgagctg ctcgacttca tcgccaccac cgcgccgtac
     2881 cgcagtgaca cggagtcgcg gcggcagatg ctgctgcggg gaccgggcgg cgtggcgctc
     2941 gacgaacccg atgagcccgc tctcctgggc tgactcctca gctcggaccc ggacccgccg
     3001 acaggaggtc cgtggcacga cgatcgacga caagctccat cctggctgcc ggaggccgac
     3061 tcccggtcct ggtcctgcgt cgggacccga ccgactcggt cctgatcgag cagcacctgg
     3121 aacgctccaa ccgactcgac gtccaggtgg tgcgaccggc ggacctggtg cgtaccccgc
     3181 tgcgctggcc agccgatccc aggatcgtcc cgctcgcggt ctgcgtcctg ttccgcgacc
     3241 ggccgggctc gatcgccgcg gccagccgcc tggtcgagca cctgggcgac gttccggtgg
     3301 tcggagtgct cgacgctccc tatcccgatg agccgaccgc cgagatggcc gccgagctgg
     3361 tcaccgctgg tgtggccgac gtcatcgaca tcgaccacct gtccgcgcac gtcctggagc
     3421 aggtgctgct gggggcgatc gaccgcagcg tcgcgggcgt ggtgcacctg ggtgactcgg
     3481 tctccgtgct cgacgacgag caccgccgcg ccgcccgctg agcggccggt cgggttccgg
     3541 tcaggtcccg gtgctccgtc gccggtggcc gctgccgagt cccttcccca tgagctcgaa
     3601 gcacaggtcc acccgggtgg ggagatcgat cgagggatcg gccagccact gctggatcga
     3661 tgctcgcatg gcggcgagcg ccgcggccgc gaggagctcg ggagagggcg actcgtggtc
     3721 cgcgtcctga acgcgtgacg agaggagctc ggcgaggtcg tgctcccaca tggactggag
     3781 cagcaggctc ctcgccgcca ccgtcggagc ttcggccatc atccgaagac gcttcgacat
     3841 cgcgtcgcgg tgtccggagt cgtctgaggc cacggcacga aatgcctggc gcagggactc
     3901 ccagggttcc tcgcctgctg gtcgcgctga gagtcgttcg agcaggaggc ggagtcgatc
     3961 cgcgtagtcg gccaggacga cgtcctcctt ggtctcgaag tagtggaaga aggtgcgcgg
     4021 tgcgacctcc gcgtgtcgcg cgatgccctc gatcgtcgtg gcgtcgaagc cgtcgcgggc
     4081 gaacagctct gtggctgcat cctcgatgcg acgtcgcgtc tgccgcttct tcttctcgcg
     4141 tagacccggt tcgtccatgg gtcgagcgta gtagcacgac aggcactgca aacatgcaat
     4201 gaacgtaaaa ctgcagtcaa tgcatctttg cgctactctg tccctgtctc gtgcgagccc
     4261 cagaaggacg gtggcgaatg acgaactggc tgtaccggat cggccgggga tccgctgggc
     4321 gcaggtggct ggtggtcttc ggctgggtcc tgctccttgc gacactcgcc atcacgaacc
     4381 gcgccgtcgg cggccagccc gtggattccc tcgaggttcc cggtgtcgag tcgcaacagg
     4441 cagtcgacct cctccgccaa cggttccccg aacgggccgg cgcgaccgcg atggtggtct
     4501 tccacacgca gaccaccacg atcgactcgc ggagcgccgc ggatgccgtc tcggccacgg
     4561 ttgccgacct ccagacgctg cctcacgtca tctcggtcac cgacccgctc acgaccccgg
     4621 gcgcaatcgc cccggaccgc agcaccgctg tggcggcgct gcagtacgac cgctccaccg
     4681 ccgacctggg tcatgcggcg ctggacgcgc tcgtcgccag cggtaccacg gccgagcgtg
     4741 cgggtgtgca ggtcgagttc ggcggtgagc ttccgacggt cctgaagaag gaatcgaccg
     4801 ggccggctga ggccatcggg gtggtcgccg cgctcgtcat cctcttcgtg accttccgca
     4861 ccgtggcgtc gatggcactg cctctcctca ccgctgcgac cgggctgctg gccgggttgt
     4921 ccgccgtcgg tctgctgtcc ggactgatcg aggtgcccac cgtcgccccg cggttgggca
     4981 ccatgatcgg actgggcgtg ggtatcgact acgcgctgtt cgtcctcagt cgccaccgtg
     5041 acaacctggc gtccgggatg ccgatcgacg agtcgatcgg caggaccaac gcaaccgccg
     5101 gcaaggcggt cgtcatcgcc ggcgcaacgg tggtggtcgc catcctcggc ctgcagttcg
     5161 cgggcatccc gttcgtcgcc gcactcggct actcagcggc gatcgtcgtc acctttgctg
     5221 tcgtggttgc catcaccctg ctcccggcac tcctcgcgct cgtcggtgag cgggtgctcc
     5281 cccgatcgtg gcgtgcgtct caccgtcccg tcgtggagca ggccgccccg acgggcgagg
     5341 gaccgactgc cacgaggtgg gtccgatggg cctcgtgggt cacacaccgc ccctggcgat
     5401 ccgcgatcgc ggcgacagcg gtgcttctca tcgcggccgt ccccctgatc gacatgcagc
     5461 tcggtcaggc cgacgccggc acccagccgg cggacacgac ccaacgccga gcctacgacc
     5521 tactcgcagc cggctacgga gccggcttca acggcccgct cctgctcgtc gtcgacctcg
     5581 gccgatcgcc ggatcttcag gtcgtcacgc tgatccgcga ggccgtggcg acagatcccg
     5641 gaatcggctt cgtgtcaccg ccgacgacga actcggcgca ggacaccgtg gtcctgaccg
     5701 ccgtgccgac gaccgaacct caggcccgcg ccaccagcga actggttcac cggctccgtg
     5761 ggaacacgct gccgtcggcg gtcggggaca gcggggcgaa ggtgctcgtc ggcgggccga
     5821 ccgcgtcgtt catcgaccag tcggaccgaa tcgccgaacg gcttccccgg ttcatctccg
     5881 cggtggttgc gatgtcgttc ctgctgctga tgatcgtctt ccgctcgatc ctcgtgccgc
     5941 tcaaggctgc gctgctgaac ctcttgtcga tcggagccgc gtacggcgtg gtcgttgcgg
     6001 tgttccagtg gggttggggt cgctccctca tcggactcga tgaggccgtc ccgatcgcgt
     6061 cgttcgtgcc gatgatgatg ttcgcgatcc tgttcggcct gtcgatggac tacgaggtgt
     6121 tcatcctctc ccgcatccgc gaggagcacc ttgccggtcg ctcgaacaac gacagcgtcg
     6181 tgaccggtct cggggcgacc gcgaaggtca tcaccgcagc ggcgctcatc atggtcagcg
     6241 tgttcgtcgg attcgtcatg agtccggatc cgatcgtgaa gatgatgggg atcggtctgg
     6301 ccacagctgt cgcgatcgac gcgacggtgg tgcggatggt gctgctcccg gcgacgatgg
     6361 ccctggtcgg cgaacccaac tggtggctgc cacggtggct cgatcgagtc ctcccgcacc
     6421 tcgacgtgga gggttgaggg tcctcgttgc tgttgggtcc gaccctgggg accagcaggc
     6481 acgatcagcg tcgggcggtc cgggtgtttc gccaggtccg gtagcctgcg ccgggcccaa
     6541 cggggggcac cggacgaggc aggagacggc gtgctccaac tgaccgagga acaccacctg
     6601 ttccgccaga ccgtgcgcga ggtcgtcacc aacgagatcg acgcccacgc ggacgagtgg
     6661 gagcgcgacg gcgacttccc cgcccacgac ctgttccgcc gcttcggcga gctcggcctg
     6721 ctgggcctcg agtacgaccc ggcctacggc ggcgggggag cggaccacac ctacacgatg
     6781 atcctgggcg aggagctggg ccgctcggca tcgctcggag tgtccatggc gctcagcgtc
     6841 cagaccgaca tggccacccc ggctctgcac cggttcggca ccgccgagct caaggagcgc
     6901 tacctgcgcc ccgcgatcgc cggcgagcac gtcgccgcca tcggcgtgtc ggagcccgac
     6961 gccggatccg acgtggccgg ggtgcgcacc cgggccgtgc gcgacggcga cgagtgggtc
     7021 atcaacggca ccaagctgtg gatcaccaac ggcacccagg ccgactggat ctgcctgctg
     7081 tgtcgcacct ccgacgaggg cggctaccgg ggcatgagcc aggtgatcgt cccgaccgac
     7141 acgcccggct tctcggtgag tcgcaagctc gacaagctcg ggatgcgggc atcggacacc
     7201 gccgagctca gcttcgacga cgtccgggtg ccggtcgcca acacgatcgg tgaggcgggg
     7261 cgcggcttcc agcagcagat ggcccagttc cagaacgagc ggatgatcgc cgcgtaccag
     7321 atggtgggtg ccaccgagct ggcgctggaa cggaccgtcg cctacctgcg tgaacgccag
     7381 gcgttcggcg ccccgctgat ggccaaccag gcgctgcagt acgagctggc cgacctggcc
     7441 gcggacctgg acctggtccg tcaccacaac tgggccgcgg tcgccgccta cctgaacggc
     7501 gaggacatca cccgccaggc gaccgtcgcc aagctcaagg ctgcccgcct ggcccgccgg
     7561 atggccgacc tggccgtcca gttccacggc gggatgggct acgtcgagga gaactggccg
     7621 gcccggttct tccgcgacac ccgcctgtgg tcgatcggcg gcggagccga cgaggtcatg
     7681 ctgcgcaccc tggcccgcat gaacggcatc gtcggcgact ga
//
LOCUS       DAOQOD010000144         7712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3557_length_7712_cov_12.456445, whole genome shotgun sequence.
ACCESSION   DAOQOD010000144 DAOQOD010000000
VERSION     DAOQOD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_7712_cov_12.456445"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLZ72_12405"
     CDS             <1..185
                     /locus_tag="PLZ72_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37144.1"
                     /translation="DVSLETLSFEDLGDGWTRLHAQSLVDSFEGRDAWLASGMEEGID
                     QGYAKLDALLAAGAVS"
     gene            182..763
                     /locus_tag="PLZ72_12410"
     CDS             182..763
                     /locus_tag="PLZ72_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HNA37145.1"
                     /translation="MSSSVDLAPIDEYRLTAARFSEVVDGVADPADWDRRSPVPEWTA
                     RDVVGHLVDWFPAFLRAGTEIELPAGPSVQQDPAAAWRHLDAAVLGLLEDPATESAVL
                     VNPHIGEVPLPRAVSQFFTADVFMHTWDLARATGQDHGLDPERCETYLQQMLPMDEVL
                     RASGQYGPKVEVPADADAPTRLMAFIGRDPGNT"
     gene            926..1783
                     /locus_tag="PLZ72_12415"
     CDS             926..1783
                     /locus_tag="PLZ72_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNA37146.1"
                     /translation="MHRVVRGEHFHWVGDGFRVTNLFPSAADLGMRISPFLLLDYAAP
                     FDFEPTEHPGGVGVHPHRGFETVTLAFEGSVAHHDSTGAGGVIHPGDVQWMTAASGVL
                     HKEYHEVEWARRGGRFHMVQLWVNLPAAHKMGPPRYQGLTAEQIGRSDLPDGAGIVRV
                     VAGRYGDHVGPAETVTPINLWDLQLTGAAPVQLSFPRDETVALLVLDGSVTVNGTAAN
                     HRDLVVLDDDGTDLTVDAAEPARLLLLNGTRIDEPVVAYGPFVMNTRTQIMEAIQDFE
                     SGAFGHLDD"
     gene            1823..2254
                     /locus_tag="PLZ72_12420"
     CDS             1823..2254
                     /locus_tag="PLZ72_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37147.1"
                     /translation="MTDGSRNLDDLVWLRRVRDRIDREYAQPLDVEALARGAHMSAGH
                     LSREFKRAFGESPYSYLMTRRIERAMALLRRGDTTVTDACFAVGCSSLGTFSTRFREL
                     VGMSPSEYRRRAESDEGVPPCISRQVSRPIRNREAAGPAGP"
     gene            2265..>2647
                     /locus_tag="PLZ72_12425"
     CDS             2265..>2647
                     /locus_tag="PLZ72_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA37148.1"
                     /translation="MDIAIHNAFLPHTDPEASLAFYRDTLGFEVRNDVGGGTMRWLTL
                     GVPGQDVSLVLTPPAVDPGITDDERRAVTEMMAKGTFAILTLASRDLDGTFDELAAAG
                     VEVVQEPTDQPYGIRDFAVRDPAGNL"
     assembly_gap    2648..2747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2748..3473
                     /locus_tag="PLZ72_12430"
     CDS             <2748..3473
                     /locus_tag="PLZ72_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNA37149.1"
                     /translation="MRDELRDVWTSFRYDDEVNCIVLSGAGHSFCTGIDRAEAVSDDN
                     NEAMADGVYPGYPTPWMYDDPGRDVGPKSCDLWKPVIGAVQGMACGGAFYMLGETEFI
                     ISSDDATFFDPHVTYGMTAAFEPIQMLSKMPFPEVMRMSLMGVHERIGAERAREIGLV
                     TEVVARDDLMERTLAVAQIIADSPPLVVQGTLRALWTAFDTSRSQAIDLANLFTRIGS
                     DAAAFKAGQERFASGERPNWIVR"
     gene            complement(3572..5173)
                     /locus_tag="PLZ72_12435"
     CDS             complement(3572..5173)
                     /locus_tag="PLZ72_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37150.1"
                     /translation="MDETASYSLPMHSGASSRFLIGVAAVLATAGLLAGCSGSDDEGG
                     SGSDGGRPSSTVVASDADVVLAVDGDDAVAHVDDRFQSYNIEMVEVTGGEFWKPYDSG
                     PGKVTRPPIDLTSERLRNLARGLGPAYIRVSGSWANTTYFDPEGTSGGVKPEGFGGVL
                     TGDQWKGVGDFARAVDGEVVTSFASGTGVRDAAGVWQPDQARSLLEFSKANDVPVVAA
                     ELVNEPSIPLGVPAGYDAAAFGRDFTTFKEMVDDVMPDLRIVGPGAVEDVTPIILKGP
                     AIPAEDMFKAADGDFDVVSYHFYPKVSERCGSKEGPEVALTQEFLSRIETDKDFYEGL
                     RDQYQPDAPMWVTEIAQAACGGDRWASTYRDVIRYVDSNGRLATGDGDAMFHNTLAAS
                     DYALLDEDGLVPRPNYWAAVLWHRLMGPAVLDVAHHPAPDLSVYAHCTPGAEDPSVTY
                     AVVNSSETETRTVATGSGSAEVYLLTSDSLDSGSISLNGTVLAAADDGTLPPMEAEKA
                     TGSVEVPPASVAFVVEPTDVRACSR"
     gene            5282..7645
                     /locus_tag="PLZ72_12440"
     CDS             5282..7645
                     /locus_tag="PLZ72_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNA37151.1"
                     /translation="MSPTGHPADTHDTIRVTGARVNNLRDVSVEIPKRRLTVFTGVSG
                     SGKSSLVFGTIAAESQRLINETYSTFVQGFMPSMARPDVDVLDGLTTAILVDQERMGG
                     DVRSTVGTATDAHAMLRILWSRLGEPHVGPPSAFSFNVATISGAGAVTLQKGGTTTRE
                     KRSFTRLGGMCPRCEGRGQVTDFDLSALYDESKSINEGALTVPNYSVDGWYGRIFRGC
                     GFFDPDKPIRRFTKRELQTLLYKEPTRIKVEGVNVTYEGLIPRIQKSMLSKDPESMQP
                     HIRAFVERAITFGTCPDCDGTRLGEAARSSKVAGMSIAEACDLQITDLAEWIRSLDEP
                     SVAPLLTSLAETMDSFVEIGLGYLSLNRPASTLSGGESQRTRMIRHLGSSLTDVTYVF
                     DEPTAGLHPHDIDRMNELLRRLRDKGNTVLVVEHKPEVIEIADHVVDLGPGAGSSGGT
                     ICFEGDVAGLRSSGTVTGRHLDDRAVIKEDVRSFTGVLEIRGADTHNLVDVDVDVPLG
                     VLVVVTGVAGSGKSSLIDGSVSGRDGVVTVDQSAIKGSRRSNPATYTGLLEPIRKAFA
                     KANGVKPALFSPNSEGACPTCNGAGVVYTDLGVMASVATTCEDCDGKRFDASVLEYFL
                     AGRNIAQVLAMSVDDALEFFADGDARTPAAQRILERLHDVGLGYLTIGQPLTTLSGGE
                     RQRLKLATHLGEKGGIFVLDEPTTGLHLADVEQLLGLLDRLVDAGRSVIVIEHHQAVM
                     AHADWIIDLGPGAGHDGGRVVFEGTPADLVAARSTLTGQHLADYVSA"
BASE COUNT         1147 a         2688 c         2628 g         1149 t
ORIGIN      
        1 acgacgtctc gctcgagacg ttgagcttcg aggacctcgg cgacggctgg acgcgcctgc
       61 acgcccagtc cctcgtcgac agcttcgagg gccgcgacgc ctggctggcc tcggggatgg
      121 aggagggcat cgaccagggc tacgccaagc tcgacgccct cctcgctgcc ggagccgtgt
      181 cgtgagcagc agcgtcgacc tcgctccgat cgacgagtac cggctgaccg cggcgcggtt
      241 ctcggaggtg gtcgacggcg tcgccgatcc ggccgactgg gaccggcggt cgccggtgcc
      301 cgagtggacc gcccgcgacg tggtcggcca cctggtcgac tggttcccgg cgttcctccg
      361 agccgggacc gagatcgagc tcccggccgg cccgtcggtg cagcaggacc ccgccgccgc
      421 atggcgccac ctcgacgcgg cggtcctcgg tctgctcgag gatccggcga cggagtcggc
      481 ggtcctggtg aaccctcaca tcggcgaggt tccgctgccg agggcggtgt cgcagttctt
      541 caccgcagac gtgttcatgc acacctggga cctggcccgg gccaccggcc aggaccacgg
      601 gctggatccc gagcggtgcg agacctacct gcagcagatg ctgcccatgg acgaggtgct
      661 gcgtgccagc ggccagtacg gcccgaaggt cgaggtgccc gccgacgccg atgcgccgac
      721 ccgcctgatg gcgttcatcg gacgcgaccc cgggaatact tgatacatca agtattgttt
      781 gacatggtgc gcaccgaggg tgccgagacc ggaggtcacc atgtccgtca acgacacgct
      841 ctctgacaac gcaaccctgt ctgaccacgc gacgcggcca gagaacgacg atccgcatgt
      901 cagcgacaca cagaccctgc ggtcggtgca ccgggtggtc cgcggcgagc acttccactg
      961 ggtcggcgac gggttccggg tcaccaacct gttcccctcc gccgcggacc tgggcatgcg
     1021 gatcagcccg ttcctgctgc tcgactacgc cgcgcccttc gacttcgagc cgaccgagca
     1081 ccccggcggc gtgggcgtgc acccgcaccg ggggttcgag acggtcaccc tcgcgttcga
     1141 gggcagcgtg gcccaccacg acagcaccgg cgccggcggg gtcatccacc ccggtgacgt
     1201 gcagtggatg accgccgcgt cgggcgtcct gcacaaggag taccacgagg tcgagtgggc
     1261 ccgacgcggc ggtcggttcc acatggtgca gctgtgggtc aacctcccgg ccgctcacaa
     1321 gatgggtccg ccccgctacc agggcctgac cgcggagcag atcggtcgca gcgacctgcc
     1381 cgacggcgcc gggatcgtca gggtcgtcgc cgggcgctac ggcgaccacg tcggccccgc
     1441 cgagacggtg acgcccatca acctgtggga cctgcagctg acgggtgccg ctcccgtgca
     1501 gctgtcgttc ccccgcgacg agaccgtcgc cctgctggtg ctcgacgggt ccgtcaccgt
     1561 caacgggacc gcggccaacc accgcgacct ggtcgtgctc gacgacgacg gcaccgacct
     1621 gaccgtggac gcggccgaac ccgctcgact gctcctgctc aacggcaccc ggatcgacga
     1681 acccgtcgtc gcctacggac cgttcgtcat gaacacccgc acccagatca tggaggcgat
     1741 ccaggacttc gagtcgggcg cgttcggaca cctcgacgac tgatctcgcc gggtcgggcc
     1801 cggacgtgct cggatggctc ccgtgaccga cggctcccgc aacctcgatg atctcgtctg
     1861 gctgcgacgg gtccgggacc gcatcgaccg cgagtacgcc cagccgctgg atgtggaggc
     1921 cctcgcccgg ggtgcgcaca tgtccgccgg gcatctcagc cgggagttca agcgcgcgtt
     1981 cggggagtcg ccgtactcgt atctgatgac ccgacgcatc gagcgggcca tggcgctgtt
     2041 gcgccgcggc gacaccaccg tcaccgacgc ctgcttcgcg gtcgggtgct cctctctcgg
     2101 cacgttcagc acccggttcc gcgagctggt gggcatgtcg ccgagcgagt accgccgccg
     2161 ggccgagtcc gacgaggggg tgccgccgtg catctcacgt caggtgagca gaccgatcag
     2221 gaatcgagaa gccgccggtc ccgccggtcc gtaggttggc tcccatggac atcgccattc
     2281 acaacgcctt ccttccccac accgatccgg aggcctctct cgccttctac cgcgacaccc
     2341 tcggcttcga ggtgcgcaac gacgtcggag gcgggaccat gcgctggctc acgctcggtg
     2401 tccccggaca ggacgtgtcg ctggtgctga cccctccggc ggtcgacccg ggcatcaccg
     2461 acgacgaacg ccgggcggtg accgagatga tggccaaggg caccttcgcc atcctgaccc
     2521 tggccagccg ggacctcgac ggcacgttcg acgagctcgc cgcggcgggg gtcgaggtcg
     2581 tccaggagcc gaccgaccag ccctacggga tccgtgactt cgccgtgcgt gacccggccg
     2641 gcaacctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg cgcgacgagc
     2761 tgcgcgacgt gtggacgtcg ttccgctacg acgacgaggt caactgcatc gtcctgtcgg
     2821 gggcgggcca ctcgttctgc accggcatcg accgcgccga ggccgtctcc gacgacaaca
     2881 acgaggccat ggccgacggc gtgtaccccg gctacccgac gccgtggatg tacgacgatc
     2941 ccggccgcga cgtcggcccc aagagctgcg acctgtggaa gccggtcatc ggcgccgtgc
     3001 aggggatggc gtgcggcggg gcgttctaca tgctgggcga gaccgagttc atcatctcct
     3061 ccgacgacgc caccttcttc gacccgcacg tcacctacgg gatgaccgcg gcgttcgagc
     3121 cgatccagat gctgtcgaag atgccgttcc ccgaggtcat gcgcatgtcg ctgatgggtg
     3181 tccacgagcg catcggtgcc gagcgggccc gcgagatcgg gctggtcacc gaggtggtcg
     3241 cccgcgacga cctgatggag cggacgctcg ccgtggcgca gatcatcgcc gactcgccgc
     3301 cgctggtggt ccaggggacg ctgcgggcgc tgtggaccgc gttcgacacg tctcgctccc
     3361 aggcgatcga cctggccaac ctgttcaccc gcatcggctc cgacgccgcc gcgttcaagg
     3421 cgggtcagga gcgcttcgcc tcgggtgagc gacccaactg gatcgtccgc tgacgcgctg
     3481 atcgctcagc gcatgtcggt cgcggagaag tcctcggtgc cggcgaacgg cagcagttcg
     3541 ggacgaccga tccggcgcgg gtcgtcgtcg gtcatcggga gcaggcccgg acgtcggtcg
     3601 gctcgaccac gaaggcgacg ctcgccgggg ggacctcgac cgatccggtc gccttctcgg
     3661 cctccatcgg gggcagggtg ccgtcgtcgg ccgcggcgag gaccgtgccg ttgagcgaga
     3721 tcgatccgga gtcgagcgag tcgctggtca gcaggtacac ctcggcggac ccggaaccgg
     3781 tggcgacggt gcgggtctcg gtctccgagg agttcaccac cgcgtaggtg acgctcgggt
     3841 cctcggcacc gggcgtgcag tgggcgtaca ccgacaggtc cggcgccggg tggtgcgcga
     3901 cgtcgaggac ggccggtccc atcaggcggt gccacagcac agcggcccag tagttcgggc
     3961 gggggaccag gccgtcctcg tccagcaggg cgtagtcgct ggcggccagc gtgttgtgga
     4021 acatggcgtc gccgtcgccg gtggccagac ggccgttgct gtcgacgtag cggatcacgt
     4081 cgcggtaggt cgacgcccag cggtccccgc cgcacgcggc ctgggcgatc tcggtgaccc
     4141 acatgggggc gtcgggctgg tactggtcac gcaggccctc gtagaagtcc ttgtcggtct
     4201 cgatcctcga caggaactcc tgggtgagag ccacctcggg tccctccttc gacccgcagc
     4261 gctccgacac cttcgggtag aagtggtagc tgaccacgtc gaagtcgccg tcggcggcct
     4321 tgaacatgtc ctccgcgggt atcgccgggc ccttcaggat gatcggcgtc acgtcctcca
     4381 ccgctccggg gccgacgatc cgcagatccg gcatgacgtc gtcgaccatc tccttgaacg
     4441 tcgtgaagtc ccgtccgaac gcggcggcgt cgtagccggc gggcacgccg agagggatgc
     4501 tcggctcgtt gacgagctcg gcggcgacga cgggcacgtc gttggccttg ctgaactcga
     4561 gcagcgagcg ggcctggtcg ggctgccaca ccccggccgc gtcgcgcacg cccgtgccgg
     4621 aggcgaacga ggtgaccacc tcgccgtcga ccgcccgggc gaagtcgccg acgcccttcc
     4681 actgatcgcc ggtcagcacc ccgccgaagc cctcgggttt gaccccgccc gaggtcccct
     4741 cgggatcgaa gtacgtggtg ttggcccacg acccgctgac ccggatgtag gccggaccga
     4801 gcccgcgggc caggttgcgc agacgctcgg aggtgaggtc gatcggtggt cgggtgacct
     4861 tgcccggtcc cgagtcgtac ggcttccaga actcgccgcc ggtcacctcg accatctcga
     4921 tgttgtagga ctggaagcgg tcgtcgacgt gcgcgaccgc gtcgtcgccg tcgacggcca
     4981 gcaccacgtc cgcgtcggac gcgacgacgg tgctcgacgg tcggccaccg tcggaacccg
     5041 atccgccctc gtcgtcggac ccgctgcagc cggccagcag gccggccgtg gccaggaccg
     5101 ccgcgacgcc gatgagaaac cgtgagctcg cccccgagtg catcgggagg gagtagctgg
     5161 ccgtttcgtc catcccgggc ccgacgcgac ggtcaggatc cgagaagtcg gtgcggcggg
     5221 ccgcgtcgta gcgtgcgtcg gcggcatggg aacgacccgt cgccaccgac gaggagtcga
     5281 tgtgagcccg acaggacatc cggcggacac ccacgacacg atccgcgtca cgggagcccg
     5341 ggtcaacaac ctgcgcgacg tctccgtcga gatccccaag cgtcgcctga cggtgttcac
     5401 cggcgtgtcg gggtcgggca agagctcgct cgtgttcggc acgatcgcgg ccgagtccca
     5461 acggctgatc aacgagacct acagcacgtt cgtccagggg ttcatgccgt cgatggcccg
     5521 acccgacgtc gacgtgctcg acgggctgac caccgcgatc ctcgtcgacc aggagcgcat
     5581 gggtggcgac gtccgctcca ccgtcggcac cgccaccgac gcccacgcca tgctgcggat
     5641 cctgtggagc cggctcggcg agccgcacgt cggtccgccg agcgcgttct cgttcaacgt
     5701 ggccaccatc agcggcgccg gcgcggtgac gttgcagaag ggcggcacca ccacccggga
     5761 gaagcgcagc ttcacccgcc tgggcggcat gtgcccccgc tgcgaaggtc gcggtcaggt
     5821 caccgacttc gacctgagcg cgctctacga cgaatccaag tcgatcaacg agggcgctct
     5881 caccgtcccc aactacagcg tcgacggctg gtacggccgg atcttccgcg gctgcgggtt
     5941 cttcgacccc gacaagccga tccggcgctt cacgaagcgg gagctccaga cgctgctcta
     6001 caaggagccg acccggatca aggtcgaggg cgtgaacgtc acctacgagg gcctcatccc
     6061 ccgcatccag aagtccatgc tgtccaagga ccccgagtcg atgcagcccc acatccgagc
     6121 cttcgtcgag agggcgatca ccttcgggac ctgcccggac tgcgacggga cccgcctggg
     6181 tgaggcggcc cggtcgagca aggtcgccgg gatgagcatc gccgaggcgt gcgacctgca
     6241 gatcaccgac ctggccgagt ggatccgctc gctcgacgag ccgtcggtcg cgccgctgct
     6301 gacatcgttg gccgagacga tggactcgtt cgtcgagatc ggactcggct acctcagcct
     6361 caatcgaccg gcgtcgaccc tgtcgggcgg cgagtcgcag cgcacccgga tgatccgcca
     6421 cctcggctcg tcgctgaccg acgtcaccta cgtgttcgac gagccgaccg ccggtctgca
     6481 cccccacgac atcgaccgca tgaacgagct gctgcgacgg ttgcgcgaca agggcaacac
     6541 cgtgctggtg gtggagcaca agccggaggt gatcgagatc gccgatcacg tggtcgacct
     6601 cggcccgggt gccggatcgt cgggtggcac gatctgcttc gagggagatg tcgcggggct
     6661 gcggtcgagc ggcaccgtga ccggccggca cctcgacgac cgcgccgtga tcaaggagga
     6721 cgttcgcagc ttcaccgggg tgctggagat ccgcggtgcg gacacccaca acctcgtcga
     6781 cgtcgacgtc gacgtcccgc tcggggtcct ggtcgtggtg accggcgtcg ccggctccgg
     6841 caagagctcg ctcatcgacg gatcggtctc gggccgtgac ggtgtggtga ccgtcgacca
     6901 gtccgccatc aaggggtccc gtcgcagcaa cccggccacc tacacgggcc tgctcgagcc
     6961 gatccgcaag gcgttcgcca aggccaacgg ggtcaagccc gcgttgttca gccccaactc
     7021 cgagggggcc tgtccgacct gcaacggtgc gggtgtggtg tacaccgacc tgggggtcat
     7081 ggcgtcggtc gccacgacct gcgaggactg cgacggcaag cggttcgacg cgtcggtgct
     7141 cgagtacttc ctcgccggtc ggaacatcgc gcaggtgctg gccatgtcgg tcgacgacgc
     7201 cctggagttc ttcgccgacg gcgacgcccg cacccccgcc gcgcagcgga tcctggagcg
     7261 gctgcacgac gtcgggctcg gctacctcac gatcggccag ccgctgacca cgctgtcggg
     7321 gggtgaacgc caacgtctca agctcgccac gcatctgggc gagaagggcg ggatcttcgt
     7381 cctcgacgag ccgaccacgg gactgcacct ggccgacgtc gagcagctgc tcggattgct
     7441 cgaccgcctg gtcgatgcgg ggcggtcggt catcgtcatc gagcaccacc aggcggtcat
     7501 ggcacacgcg gactggatca tcgacctcgg ccccggtgcc ggccacgacg gcggtcgggt
     7561 ggtcttcgag gggaccccgg cggatctggt ggcggcgcgc tcgacgctca ccggtcagca
     7621 cctggccgac tacgtctcgg cctgacccgc cgatgtcgta gctggcggtc gctgtggtgg
     7681 cgtgtgggct acaagttcgg tgggcggcgg cg
//
LOCUS       DAOQOD010000145         7700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3558_length_7700_cov_14.346239, whole genome shotgun sequence.
ACCESSION   DAOQOD010000145 DAOQOD010000000
VERSION     DAOQOD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_7700_cov_14.346239"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLZ72_12445"
     CDS             complement(<1..1246)
                     /locus_tag="PLZ72_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37152.1"
                     /translation="MDNTVVREFSKRRNEIDDALAELEAEIGRGAHPNEVEHVVLRTR
                     PAKNHTPAHDLVATWRERAAKHGLDADALAAVTGHEPHVQEVDTDALFASLAEPDGIC
                     AGGSVFSRSEALAAMAAHPVPSADDSTPQPLLCGAARLIEMTDQFLASPHVVALTDND
                     EPLYTTVEMLDTQEWIESRFTRGLHRGAHLVPDDHVDAALAQHGHLTSEQRRLVTEWC
                     QRGHRFQAAIGRAGAGKTTTVAACADAWTAAGYRVLGAAVKGEATRTLAAATGIECET
                     VAWYLAHTDPQQLPLDARTILVVDEASTVSDRDLDTLMDMAATTGASLRLIGDPAQHG
                     AIAAGGMFRVLCERHERDTPELVTTHRLQDPHDRAAAQALREGNIDEAFDRLAAAGHL
                     HVVADDLAMYREVLGRWWDAHRD"
     gene            1133..2197
                     /locus_tag="PLZ72_12450"
     CDS             1133..2197
                     /locus_tag="PLZ72_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37153.1"
                     /translation="MFDLVGVRATTDLGFELGECVIDLVAPLGELTDDRVVDAGALHG
                     AINSSYGKGLQAQLALGRWEHRKFGVASDRLVEGLDAAMSVDGRVGQQLRAVISGTRP
                     FVEAIAADWMDRNFDGLFGGRAGRLALAQTLKYSRPTPAFYARSFELLLGAARRNDDN
                     AVAWLLIAHLWNEPGYTYDAIVNGLRDSPSPRSTLADEVARLGQGLPDDQREVTERAV
                     AFSRRLIADAGTRIGPEDLTGLGRWAMAIELDTEGWLELTEGSVAKTGGQIAYAVEVA
                     DRCSKVQPSSAALRILLAVLGHGDAWEQHHIDGVALEALRSAADGGIDDEAFVHLRER
                     LIQRGRHEATNIRSQRTDDA"
     gene            2300..2677
                     /locus_tag="PLZ72_12455"
     CDS             2300..2677
                     /locus_tag="PLZ72_12455"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA37154.1"
                     /translation="MITHSVSVWDVEVTDQFVEWWSTLTDDQREAVTARVDLLGERGP
                     DLGRPVVDRIQSSRHQTMKELRAAKDGAIRVLFSFDPRRQAILLLGGDKSGEWNKWYE
                     WAVPLADDLYDEYLEELRTEGLI"
     gene            2677..3006
                     /locus_tag="PLZ72_12460"
     CDS             2677..3006
                     /locus_tag="PLZ72_12460"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNA37155.1"
                     /translation="MARTKFSTLRDDVTAKDGASERIAAMRAEAIEEIRLFELRHREA
                     VSQAELAGRLDVTQGAISKLEHADDVRVSTLRQYLEALGARLELVAVFDDEKRSVPVH
                     LGRDSAA"
     gene            complement(3080..3664)
                     /locus_tag="PLZ72_12465"
     CDS             complement(3080..3664)
                     /locus_tag="PLZ72_12465"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNA37156.1"
                     /translation="MPRLRLHAKLVPYRFEMFTAGLPQAMGYRTEVTPASGDGDVDVI
                     AYRDPLGLEPPDIKVQCKRTLNTIGGPDVQKLMGALAPGSTEVGLFITLGTFTVPSMR
                     EGRAPQVGSLEPVAGSPACAASRSQPVCTSAPGPALGPAQQQRPVEQRVERVRHLARR
                     RRRWLRLQRHTGVHPSYRTVTGAYRPLLPHLAEI"
     gene            3639..3809
                     /locus_tag="PLZ72_12470"
     CDS             3639..3809
                     /locus_tag="PLZ72_12470"
                     /inference="COORDINATES: protein motif:HMM:NF027850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37157.1"
                     /translation="MACSRSLGKKRFLAQREFALGIEALERFVAGEPLHHLHECVVGV
                     LAMESEPVDPRP"
     gene            3874..4197
                     /locus_tag="PLZ72_12475"
     CDS             3874..4197
                     /locus_tag="PLZ72_12475"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNA37158.1"
                     /translation="MAELELHDEIVDWMASLDDTEWDRTVVVIDRLATLGSSARMPLS
                     KSLGDGLFELRFTLGPTARRITYRFTKDGRIILLTTFRKQRNNERNEITRARKAAEDC
                     AEQYP"
     gene            4209..4529
                     /locus_tag="PLZ72_12480"
     CDS             4209..4529
                     /locus_tag="PLZ72_12480"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37159.1"
                     /translation="MANYSRWDDVKKKRPGPDADTRAGVEQDLALGQLIYDLRTEAGL
                     SQRELAERMGTTQSVISRLEEGGGARNRIDTLARVATALDRHLVVSFPEKVPAKLKDA
                     VQVA"
     gene            complement(4933..5061)
                     /locus_tag="PLZ72_12485"
     CDS             complement(4933..5061)
                     /locus_tag="PLZ72_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37160.1"
                     /translation="MTPASGDGVDDIAYRDPLGLEPPIIKVQCKRTLNTIGGPDVQ"
     gene            complement(5351..5800)
                     /locus_tag="PLZ72_12490"
     CDS             complement(5351..5800)
                     /locus_tag="PLZ72_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37161.1"
                     /translation="MLQFRNVDASPSDDITTWPFEALATAIDRGLVADWQPIIAEIRR
                     APWGAVTRRVEHHLDHRDADGTTAIFRLAVGRARHDAEQAERAEVARRVRRSIERSGL
                     TSAEFARAVGTSASRLSTYGTGRVVPSAAMLVRIERAGGLDPSAASR"
     gene            complement(5800..6459)
                     /locus_tag="PLZ72_12495"
     CDS             complement(5800..6459)
                     /locus_tag="PLZ72_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37162.1"
                     /translation="MLVSAARLQEIVPDAVLVGDSAAALYAGHRDSFDHDHVLTDLID
                     RYATVLDAVEATEGWATSVRASKPPFTIMGRLGGIEAGLRQMRRTRQLEPCEIDLGDD
                     TVVVAPTAAEALRIKAYLVVQRNVVRDYLDVVALAEHLGLKQAVEILLHIDDYYADRS
                     DETGSVLTSLVVALAQPHPRDSEVTRELPRYRALDPRWHEWSSVVEVCQELALRISGA
                     V"
     gene            6838..7221
                     /locus_tag="PLZ72_12500"
     CDS             6838..7221
                     /locus_tag="PLZ72_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37163.1"
                     /translation="MTDILTRDADKLHRALARGGDEVRLTVSRETAEWMSQLVDAKVS
                     GRDVVLTNDRGEVTPTQAGQMLGISRPQVRKLMDDGRLDFRKVGTHHRITVASIQAFL
                     VAERERRAPALAELARLQNDAGLVE"
     gene            7218..>7700
                     /locus_tag="PLZ72_12505"
     CDS             7218..>7700
                     /locus_tag="PLZ72_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNA37164.1"
                     /translation="MTGEATEVFGPIKVVFADANVLYSRVLRDYLVYAADEGVIAIRW
                     SRAILTEAVEHLQANNVAFDDEQADLLIRLLNTAYPHAEVKPTATARRKVNELDMPDE
                     DDRHVLAAAVSARAEVLCTNNVKDFPPEAMASVGIELLTADALLSRLVTMHPSRMRDA
                     H"
BASE COUNT         1285 a         2511 c         2666 g         1238 t
ORIGIN      
        1 cgtcgcggtg ggcgtcccac cagcggccga gcacctcgcg gtacatggcg aggtcatccg
       61 cgacgacgtg gaggtgcccg gccgcggcga gacggtcgaa cgcttcgtcg atgttgccct
      121 cgcggagcgc ttgggcagcg gcgcggtcgt gggggtcttg gaggcggtgg gtggtgacca
      181 gctcgggggt gtcgcgctcg tggcgctcgc agagcacgcg gaacattccc ccggcggcga
      241 tggcgccgtg ctgggctggg tcgccgatga gccggaggct cgcgccggtg gtggcggcca
      301 tgtccatgag ggtgtcgagg tcgcggtctg agacggtgga ggcttcgtcg acgacgagga
      361 tcgtccgggc gtcgagcgga agctgctggg ggtcggtgtg ggcgaggtac caggcgaccg
      421 tctcgcactc gatcccggtc gccgccgcga gggtccgggt cgcttcgccc ttcacggcgg
      481 caccgagcac ccggtagccg gcggcggtcc atgcatccgc gcaggccgcg acggtggtgg
      541 tcttgcccgc accggcacgt ccgatggcgg cttggaagcg gtggccgcgt tggcaccact
      601 cggtcacgag ccgccgttgc tcggaggtga ggtgtccgtg ctgggcgaga gcggcgtcga
      661 cgtggtcgtc ggggacgagg tgggcgccgc ggtgcagccc tcgggtgaac cgggactcga
      721 tccactcctg ggtgtcgagc atctcgacgg tggtgtagag aggttcgtcg ttgtcggtga
      781 gcgcgacgac gtgaggcgag gcgaggaact ggtcggtcat ctcgatgagc cgcgccgcgc
      841 cgcacagcag cggttgaggt gtgctgtcgt cggcgctggg cacagggtgg gcggccatgg
      901 ctgcgagcgc ttcggagcgg gagaacaccg aacccccggc gcagatccca tccggctcag
      961 ccagcgacgc gaacagcgcg tcggtgtcga cctcttggac gtggggttcg tggccggtga
     1021 ccgcagcgag cgcgtcggcg tcgaggccgt gcttggcggc tcgctctcgc cacgtcgcga
     1081 ccagatcatg ggcgggggtg tggttcttgg ctggacgggt ccgcagcacg acgtgttcga
     1141 cctcgttggg gtgcgcgcca cgaccgatct cggcttcgag ctcggcgagt gcgtcatcga
     1201 tctcgttgcg ccgcttggag aactcacgga cgaccgtgtt gtcgatgccg gcgcacttca
     1261 cggagcgatc aacagctcct acgggaaggg ccttcaggcc cagttggccc tcggcagatg
     1321 ggagcaccgc aagttcggcg tggccagcga tcgcctcgtc gaaggtcttg atgcagctat
     1381 gtcggtcgac ggccgagtag gacagcagct ccgggcagtg atctccggaa cgcgaccgtt
     1441 cgtcgaagcg attgccgctg actggatgga ccggaacttc gacgggctct tcggaggaag
     1501 ggccggtcgg ctcgccttgg cccagacctt gaagtattcc cgcccgactc cggcgttcta
     1561 cgcacgatcg ttcgagctcc tactcggcgc agctcggcgc aacgacgaca acgccgtggc
     1621 ctggttgctc atcgcacatc tctggaacga gcccggctac acctacgacg cgatcgtcaa
     1681 cggccttcga gactcgccaa gcccgcgctc aacactcgct gacgaggtgg ctcgactcgg
     1741 acaaggcctg cccgacgacc agcgagaagt caccgaacgg gcagtggcgt tctctcgtcg
     1801 gctcatcgcc gatgccggaa ctcggatcgg gcccgaggac ctgaccggac tgggacgatg
     1861 ggctatggcg atcgagctcg acaccgaggg atggctggaa ctgacagaag ggtccgtcgc
     1921 caagaccgga ggccagatcg cctacgccgt ggaggtagcg gatcgctgct ccaaggtcca
     1981 accctcgtcg gctgctctca ggatcctcct tgccgtcctc ggacacggcg acgcttggga
     2041 gcagcaccac atcgacggag ttgcgctcga ggcgctgcga tccgcagccg atggagggat
     2101 cgacgatgaa gccttcgtgc acctccgcga gaggttgatc caacggggac gacacgaagc
     2161 aaccaacatc cggtcgcagc gaacggatga tgcgtgagag ctggtaaccc gagccctgat
     2221 tgtggatgtt ggcgaggagg ctgcgcccgg ctcggggtcg ccttccctct ggacggcaat
     2281 gagccttgac tcataatgag tgataactca tagtgtgtcg gtgtgggatg tcgaggtcac
     2341 ggaccaattc gtcgagtggt ggtcgacact gaccgacgat cagcgcgagg cggtgaccgc
     2401 ccgggtcgac ctgctcggcg aacgcggccc cgacctcggc cgaccggtcg tcgatcgcat
     2461 ccagtcatca cggcaccaga cgatgaagga gctccgggcc gccaaggacg gtgcgatacg
     2521 ggtgctgttc agcttcgacc cccgtcgcca ggcgatcctg ctgctgggtg gcgacaagag
     2581 cggcgaatgg aacaagtggt acgagtgggc ggtaccactc gccgatgacc tctacgacga
     2641 gtacctcgaa gaactccgca cggaaggact gatctgatgg cacgaaccaa gttctcgacc
     2701 cttcgtgacg acgtcactgc caaggacgga gcgtccgagc ggatcgccgc catgcgcgcc
     2761 gaggcgatcg aggagatccg cctgttcgag ctccgtcacc gcgaggcggt cagccaagca
     2821 gagctcgccg gtcgcctcga cgtcacccag ggtgcgatct ccaaactcga acacgccgat
     2881 gacgtccgcg tctcgacgct gcgccagtac ctcgaggcgc tcggcgcccg gctcgaactc
     2941 gtcgcggtct tcgacgacga gaagcgcagc gtccccgtcc atctcggcag agacagcgcg
     3001 gcctgactca gcgacagtgc ccccgctccg ccggccccaa gccacgtgtt cggtgttcgc
     3061 gcctgcgtcg cagcgtctgt cagatctctg ccagatgggg cagaagtggg cggtacgcac
     3121 cggtaacggt gcggtacgac ggatgcactc cggtatggcg ctgaagcctc aaccatcggc
     3181 gacggcgcct cgccagatgc ctgactcgtt cgacgcgttg ttcgaccggc cgctgctgct
     3241 gggcgggccc caaagcaggt cccggtgcac tcgtgcagac cggctgactt cgactcgccg
     3301 cacaggcggg ggagccagcg acgggttcca atgaaccgac ctgaggtgct cgaccttcgc
     3361 gcatagatgg gacggtgaag gtgccgaggg tgatgaacag gccgacctct gtgctgccag
     3421 gagccagagc ccccatgagc ttctgcacgt cgggcccgcc gatcgtgttg agcgtccgct
     3481 tgcactggac cttgatgtcc ggcggctcca agcccagcgg atcccggtag gcgatgacgt
     3541 cgacgtcacc gtcgccgctg gcgggggtca cctcggtgcg gtagcccatc gcttggggca
     3601 gcccggcggt gaacatctcg aagcggtagg ggacgagctt ggcgtgcagc cgcagcctcg
     3661 gcaagaagcg attcctcgcc cagcgcgagt tcgcgctcgg catcgaagca ctcgaacgct
     3721 tcgtcgccgg cgaacccctt caccacctcc acgagtgcgt cgtcggcgtc ctcgccatgg
     3781 agagcgaacc agtcgacccc cgtccctgac cggccatcgc catgcgatga tcgctcttgt
     3841 acgatcatcg cattgatgcg atagtgccga tgtgtggctg aactggagct ccacgacgag
     3901 atcgtcgact ggatggcctc cctcgacgac accgagtggg atcgaacggt cgtggtgatc
     3961 gaccggctcg ccacgctcgg ctcgtcggca cggatgccgc tctcgaagag cctcggcgac
     4021 ggactgttcg agctgcggtt cacgctcggc ccgacggcac gacggatcac ctaccggttc
     4081 accaaggacg gtcgcatcat cctgttgacc accttccgaa agcaacgcaa caacgaacgc
     4141 aacgagatca cccgggcacg caaggccgcc gaggactgcg ccgagcagta cccctgaagg
     4201 agatgacgat ggccaactac tcccgctggg acgacgtcaa gaagaagcgc cccgggcccg
     4261 atgccgacac ccgcgccggt gtcgaacagg acctcgcgct cggccagctc atctacgacc
     4321 tgcgaaccga agccggcctc agccagcgcg agctcgccga gcggatgggc accacccagt
     4381 cggtgatctc ccgtctcgag gaaggcgggg gagcgcgcaa ccgcatcgac accctcgccc
     4441 gagtggccac cgcccttgac cgccacctcg tcgtctcgtt ccccgagaag gtccccgcca
     4501 agctcaagga cgccgtccaa gtcgcctgat cgccgcagcc acgccgagta cggcagggca
     4561 gagacttctg cactcgctcc tacaccacgg gccgggattc cctggtccgt gcggtgcaga
     4621 tcgccgccgg actgtgatcc cgtcggctcc agcctgtggc gcccaccgtg agcaaccgat
     4681 cgtgagccgg taggtcctca gcctgcgtca gggtcgtgat ccagcacgta ggtgcgcttg
     4741 aggggggagc agtcgacgcc actccaggtc gagctgcccg tagtgatcga agatcatgtc
     4801 gaccagctga cggccgttca cccagcgcag gtcgtgtcga ctgcgggcca aggagatcgc
     4861 gtcgctcgtg aaggtgccga ggtgatgaac agcccgacct ctgtgctgcc aggagccaga
     4921 gtctccaatg agctactgca cgtcgggccc gccgatcgtg ttgagcgtcc gcttgcactg
     4981 gaccttgatg atcggcggct ccaagcccag cggatcccgg taggcgatgt cgtcgacgcc
     5041 gtcgccgctg gcgggggtca cctcggcgcg ggtagcgcat cgcctggagc agcccggcgg
     5101 tgaacatctc gaagcggtag gggccgaact tggtgtgccg cgcctcggaa cctctcgaca
     5161 cagcaccccg cgggtcatga ccgacaccgc tgatccgctg cagccgctgg aaccgccgac
     5221 acgggccgtc gggtccgtgc cggactcctt cccatgggca gcgatgctgc gcccgcacat
     5281 atccggtcac ggcggcggcc ggatctgcga cgccccgact cgacgagacc gcgatcggag
     5341 tccggtcgcc tcagcgtgac gcggccgacg ggtccagccc gccggcgcgt tcgatgcgga
     5401 cgagcatcgc ggcagagggc acgacccgcc cggtgccgta ggtgctcagc ctcgacgcgc
     5461 tggtgccgac cgcacgggcg aactcggcgc tggtgagacc cgatcgctcg atcgaacgac
     5521 gcacgcggcg ggcgacctcg gcccgctcgg cctgttcggc gtcgtgccga gcccgtccga
     5581 cggcgagtcg gaagatcgcc gtcgtgccgt cggcatcgcg gtggtcgagg tggtgttcga
     5641 ctcggcgcgt cacggctccc cacggggcac ggcggatctc ggcgatgatc ggttgccagt
     5701 cggcgaccag ccccctgtcg atcgcggtgg cgagcgcctc gaacggccag gtggtgatgt
     5761 cgtcggaggg cgacgcgtcg acgttgcgga actgcagcat cacacggctc cgctgatccg
     5821 cagagcgagc tcctggcaga cctcgacgac cgaggaccat tcgtgccagc gggggtccag
     5881 ggcccggtag cgcggcagct cccgggtgac ctcggagtca cgtgggtggg gttgggccaa
     5941 cgccacgacg agcgacgtga ggacggaccc cgtctcgtcg gagcgatcgg cgtagtagtc
     6001 gtcgatgtga agcaggatct cgacggcctg cttgagcccc aggtgttccg cgagggcgac
     6061 gacgtcgagg tagtcgcgga cgacgttgcg ctggacgacg aggtaggcct tgatcctgag
     6121 cgcctcggcg gcggtcggag cgacgacgac ggtgtcgtcg ccgaggtcga tctcacaggg
     6181 ctcgagttga cgggtccgac gcatctggcg gagccccgcc tcgatcccgc cgagtctgcc
     6241 catgatcgtg aacggcggct tcgacgcccg gaccgacgtc gcccacccct cggttgcttc
     6301 cacggcatcg agcaccgtcg cgtagcggtc gatcagatcc gtcaggacgt gatcgtggtc
     6361 gaacgagtcg cggtggccgg cgtagagcgc tgcggcggag tcgccgacga gcacggcgtc
     6421 ggggacgatc tcctgcaggc gggctgccga gacgagcact gctcggagcg taggagaggg
     6481 gtccggcaca ccattatcat aatcgataat tgtgcaggga tggtggcccc ctcggtcccc
     6541 gattcagcga tatggctggc aggaagggag gaggttcgac aggaccaact cggagttcgg
     6601 agtatcccgg accggctcat gactgccgat ccgggctcag gggcctccaa cgaaccccag
     6661 ggtggttcaa ccaccgctgt gacctgcgac ttgatcgcgc agtgggccca tgatcctggc
     6721 tgcctgcccc aggattgggt tgggcaggag ccgctgagtg atgggagcaa ctcccgcccc
     6781 tgcgccctgt cagatgttac ggttgattca gatagagcag atgatgcgga gatgatgatg
     6841 actgacatcc tgacccgaga cgctgacaag ttgcatcgag cgctggccag gggcggcgac
     6901 gaggttcggc tcaccgtctc gcgtgagact gccgagtgga tgtcacaact cgtcgatgcc
     6961 aaggtcagcg gccgcgacgt ggtgctcacc aacgatcgcg gtgaggtcac tccgacccag
     7021 gcaggtcaga tgctcggtat ttcgcggcct caggtccgca agctgatgga tgacggccga
     7081 ctcgacttcc gcaaggtggg cacacatcac cggatcactg tcgcctcgat ccaggccttc
     7141 ctggtggccg agcgtgagcg gcgtgccccc gccctggctg agctggcacg gctccagaac
     7201 gatgccgggc tggtggagtg acgggcgagg ccacggaggt cttcggtccg atcaaggtcg
     7261 tcttcgccga tgccaacgtg ctctactccc gcgtgctgcg ggactacctc gtctacgccg
     7321 ctgacgaggg tgtgatcgcg atccggtgga gccgagcgat cctcaccgag gcagtggagc
     7381 acctccaggc caacaacgtt gcgttcgacg acgagcaggc cgacctcctc atccggctgc
     7441 tcaacaccgc ctaccctcac gccgaggtca agccaacggc gacggcccga cgcaaggtca
     7501 acgagctcga catgcccgat gaggacgatc gccacgtcct cgcggccgcc gtgagtgctc
     7561 gagcggaggt cctgtgcacg aacaacgtca aggacttccc acccgaggcg atggcatcag
     7621 tcgggatcga gttgctcacg gccgacgcgt tgctctctcg gctcgtgacc atgcatccgt
     7681 cgaggatgcg ggacgcgcac
//
LOCUS       DAOQOD010000146         7691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3565_length_7691_cov_13.369041, whole genome shotgun sequence.
ACCESSION   DAOQOD010000146 DAOQOD010000000
VERSION     DAOQOD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_7691_cov_13.369041"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..918)
                     /locus_tag="PLZ72_12510"
     CDS             complement(79..918)
                     /locus_tag="PLZ72_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA37165.1"
                     /translation="MSEDRSEPLVTVQRPRPEVAVVTLDNPDRLNALSFRMVDDLLDA
                     LDEIHGDNSCRVVVLTGAGRGFCSGLDLSSAGGSSTSVGTSGPRAGMLSQERIAELPI
                     RIRRIQQPVIAAVNGVAYGGGFALALACDLRIAAPSARFCAQFIKIGLSGCDIGISYT
                     LPRAVGSSRAFDLILTSRVVDADEALSMGLVSAVAVDPVERALETAEVILGYSPFGVA
                     MTKEVMWANLDASSIEAAIHLENRTQILASTGGEVVEAATAFMEKRDPDWSRVSSRGV
                     WDR"
     gene            1136..1600
                     /locus_tag="PLZ72_12515"
     CDS             1136..1600
                     /locus_tag="PLZ72_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNA37166.1"
                     /translation="MKVPARVLSSAPFRWAAPKVLPPTHKLLHRLSGGRLMFDSQRQP
                     MCMLHTIGARSGEPRETPLAAVPVEDGALLVVGSNFASPRHPAWTANLLAHPDATVTF
                     RGRTYPVTARLLDDRERADRWDLLLEWFPNWRDYTEVTDREFRVFELRPVDA"
     assembly_gap    1601..1700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1865..2671)
                     /locus_tag="PLZ72_12520"
     CDS             complement(1865..2671)
                     /locus_tag="PLZ72_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37167.1"
                     /translation="MTGGVVAGGAESGSSPGPGPGAGVGPKVYIHELVDVIGHNRARY
                     MQHMTANWCPIARRERHQLCLGVWATIGSTGDWPQVVNMWELDGWEGLAANLAHETGG
                     GRDQDPSLAKWWATAASLRRGGTDRIVVPTDWTRDVTTLCADGVRGEVYAHELFTVAP
                     GAAPDLLAAAGEIARPAVATLGIEAVGGYEVAMADRSEAILIWAVPDWPGWVAYERAW
                     EPGGPLGEWSAALRRLGARWRRQLMVDAPLGPLRTGRQPQESDRRPLEEI"
     gene            complement(2668..3645)
                     /locus_tag="PLZ72_12525"
     CDS             complement(2668..3645)
                     /locus_tag="PLZ72_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA37168.1"
                     /translation="MIGLRFDMRLAPGQDVRGAYRAALDMVEWSEGSFAPFVTVSEHH
                     AVADGYLTSPGTMVAAMAARTTSTYFMVTAAILPLYDPVRLAEEMAVIDHISGGRVGW
                     VLGVGYRPEEYALLGVDFERRGRLADEHLDTLFDALAGRDLTPHGRPGSIGRAPEGRI
                     TLFWGGASRPAARRAARRGLGLFAQTNVDGLAQVYEQECAAAGIEPRGCVMPNPGAPM
                     TVFVADDVDRAWDELGEPMLRDATAYAAWNPQSAARNASMSTSRSVEELRSEGASHRV
                     VTVDGALDILGTDPLMLHPLCGGLDPEVAWPYLRRVGEQVAPARDAARS"
     assembly_gap    3673..3772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4075..5763
                     /locus_tag="PLZ72_12530"
     CDS             4075..5763
                     /locus_tag="PLZ72_12530"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HNA37169.1"
                     /translation="MGFLQPNLPVVDHEVWDHLPRQERIVPMTRHFALHGFGSPDVVI
                     LSYIVKIGIYLAVGWAFVMTTPGIDGAFDVGAWWSNPAVFFKFAFWTMLFEVLGLGCG
                     FGPLNLRFIPPLGSFLYWLRPGTIRLPPWPRRNPIAGGDTRTPFDVALYAGLLVALVV
                     TLVGAIPRWEVLTVVVLLGFIGLRDQTVFLAARGEVYGSIALAALFSAGDQVTAAKFV
                     MVIIWFGAAVSKLNRHFPFVVAAMMANSPVLRSTRLKAHLWRDAPDDLRPGRPAAVLA
                     HTGTVVELVAPMVLLLSGGGWVTTVAALVMIGFHLTILTSFPMGVPLEWNVFMIIGIV
                     VLFLGNPGLDLGALSQPVALAALVLGLLAVVVYGNLVPEQVSFLPSMRYYAGNWAATM
                     WCFRGDALERFERNVTTATLLPHQQLEKVYGSAEEAAVPLHMGYAFRGFHTHGRALWT
                     LVAEACGPDHEDYLVLDGELVAGTALGWNFGDGHLHDEQLIEALQRRCDFAPGDVRIV
                     FLESQPFHRGTQQYRLIDAATGPIGRGHVVVTDMVARQPTDADLPLHRDPGTEP"
     gene            5778..7046
                     /gene="nhaA"
                     /locus_tag="PLZ72_12535"
     CDS             5778..7046
                     /gene="nhaA"
                     /locus_tag="PLZ72_12535"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNA37170.1"
                     /translation="MTDDDDVTDDGDVTDSGDVTGPGTRRRRARALREFLDTEVAGAV
                     VLLVAVVVALVWANSSLSSSYSDLWHTELSVSVGWGLDMSMHQVVNDGLMTVFFFVVG
                     LEIKRELVDGELSDPRTAAVPVAAAIGGMVVPALLFVAINASTGTTRGWGIPMATDIA
                     FALGVLAVAGSRLASGAKLFLLTSAVVDDIGAIVVIAVVYSTSISPGALLGAAVVIGM
                     VLLARATGVQSLVVYVLLGVLLWDAMFESGVHATIAGVVLGLITPARGPDSVAERLEH
                     RLHPLSSFVIVPLFALANAGVRLDVGSLSEPAPRAVALGVVVGLVIGKTVGISAATAI
                     AVKTRIGRLPDGVGAGEVIGMAMVAGIGFTVSLFVTELAFDDPAIRDAAKTAVLVASV
                     VAAVAGGTTLWWRGRRTAESPQQARTVPDR"
     gene            complement(7091..>7691)
                     /locus_tag="PLZ72_12540"
     CDS             complement(7091..>7691)
                     /locus_tag="PLZ72_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNA37171.1"
                     /translation="GCGGSRTSGSEGGPEKPTSRKADRALRSDPYTYVPTRSGMAYVC
                     FIVDAFSRRIVGWRAASHMRTDMVLDAVEMARRSRGGGRLVGLVTHSDAGSQFTSVRF
                     TERLDEIGARPSIGTVADSYDNALAETTNGLYKAECVYGPDTTGWADVNELEHATLDW
                     VYWFNEQRLHSHCGHVPPAEFEAAFYAAQQADPVGVGNQ"
BASE COUNT         1004 a         2768 c         2583 g         1136 t
ORIGIN      
        1 cgatgccggc cccgatcccc cggctggcgc cggtgaccag cgcccggcgc ccctcgaacc
       61 gacgggcacg accggcgctc accggtccca gaccccgcgg gagctgaccc gggaccagtc
      121 ggggtcgcgc ttctccatga acgcggtcgc cgcctcgacc acctcgccgc cggtcgaggc
      181 caggatctgg gtgcggttct ccaggtggat cgcggcctcg atcgacgacg cgtcgaggtt
      241 cgcccacatg acctccttgg tcatcgccac cccgaacggg ctgtagccca ggatcacctc
      301 ggcggtctcg agcgcccgct cgaccgggtc gaccgcgacc gcggagacga gacccatcga
      361 cagcgcctcg tccgcgtcga cgacccgcga ggtgaggatc aggtcgaagg ccctcgagga
      421 cccgacggcg cgcggcagcg tgtagctgat gccgatgtcg cagccgctga ggccgatctt
      481 gatgaactgg gcgcagaagc gggcggacgg cgccgcgatc cgcaggtcgc acgccagtgc
      541 caacgcgaac ccgccgccgt aggccacgcc gttgaccgcg gcgatgaccg gctgctggat
      601 ccggcggatg cggatcggca gctcggcgat gcgctcctgg ctcaacatgc ccgcccgcgg
      661 tcccgacgtc cccaccgacg tggacgaccc gccggcgctg ctgaggtcca gaccggaaca
      721 gaagccccgg ccggcgccgg tgagcacgac cacccgacag gagttgtcgc cgtggatctc
      781 gtcgagcgcg tcgagcaggt cgtcgaccat gcggaacgac agggcgttca ggcggtcggg
      841 gttgtccagg gtcaccacgg cgacctcggg ccggggtcgt tgcacggtca ccaacggctc
      901 gctgcggtcc tcgctcacct gatcccccga tcgtcgccct ccgtcgtggg ggaagctagc
      961 gggtgccgtc ggtgcttgac cgcggccctc attcccgcga tcgtcgcccg gtgcacccgg
     1021 accgccgacc ctccgccctc acccgctgat gtacggccgg ggctccgcac cacctccacc
     1081 cgaaccgtcc cggacccaga gctggccccg agcggcgctc gcccggatca agcgggtgaa
     1141 ggtgccggcg cgggtcctgt cctcggcgcc gttccggtgg gcggccccca aggtcctgcc
     1201 ccccacccac aagctgctgc accgcctcag cggcgggcgc ctgatgttcg actcgcaacg
     1261 gcagccgatg tgcatgctgc acacgatcgg ggcccgcagc ggcgaacccc gcgagacgcc
     1321 gttggccgcg gtgcccgtcg aggacggcgc tctgctggtc gtcggctcca acttcgccag
     1381 cccccgccat ccggcgtgga ccgccaacct gctcgcccac cccgacgcga ccgtcacgtt
     1441 ccggggccgc acctacccgg tcacggcccg cctgctcgac gatcgggagc gagccgatcg
     1501 ctgggacctg ctgctcgagt ggttcccgaa ctggcgcgac tacaccgagg tcaccgaccg
     1561 cgagttccgg gtgttcgagc tccgacccgt cgatgcctga nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn tcgtcaccga cgagcgccac ccgttcccct acggcgacga
     1741 gcgcttcgga ctcccccgcc gcgacgacct tcagttcgcg gtgctgcgca aggagctcgc
     1801 gccctgatcc gtcgtcgagc ggccggcgat cagggtcctc ccgcgatcaa gggtcctcca
     1861 gcgatcagat ctcctcgagc gggcggcggt ccgactcctg gggctggcga ccggtgcgca
     1921 gcgggccgag cggcgcgtcg accatgagct ggcgccgcca ccgggcaccg agtcgacgaa
     1981 gggccgccga ccactccccg agcggcccgc cgggctccca cgcccgttcg taggcgaccc
     2041 agccgggcca gtcggggacc gcccagatga ggatggcctc ggagcggtcg gccatggcga
     2101 cctcgtagcc gccgacggcc tcgatgccga gggtggcgac cgccggccgg gcgatctccc
     2161 cggccgcggc gagcaggtcc ggcgcggcgc ccggtgccac ggtgaacagc tcgtgggcgt
     2221 agacctcgcc ccgcaccccg tccgcgcaca gggtcgtcac gtcgcgggtc cagtcggtcg
     2281 gcaccacgat gcggtcggtg ccaccccgac gcagcgacgc ggcggtcgcc caccacttcg
     2341 ccagcgacgg gtcctggtca cgtcccccgc cggtctcgtg ggcgaggttg gcagcgaggc
     2401 cctcccatcc gtcgagctcc cacatgttca ccacctgggg ccagtcgccg gtcgagccga
     2461 tcgtcgccca cacccccagg cagagctggt gtcgctcgcg gcgggcgatc gggcaccagt
     2521 tggcggtcat gtgctgcatg taccgggccc ggttgtggcc gatcacgtcg accagctcgt
     2581 ggatgtagac cttgggcccg acgcccgcac ccgggcccgg ccccggcgac gagcccgatt
     2641 cggctccccc ggccacgact cccccggtca cgaccgtgcc gcgtcccggg ccggagccac
     2701 ctgctcgccg acgcgccgca ggtacggcca cgccacctcg ggatccagtc cgccgcacaa
     2761 cgggtgcagc atcagcggat cggtcccgag gatgtcgagg gcaccgtcga cggtgaccac
     2821 acggtgcgac gcaccttcgg agcgcagctc ctcgacgctg cggctggtcg acatcgacgc
     2881 gttgcgcgcc gcggactgcg ggttccacgc cgcgtacgcg gtggcgtcgc gcagcatcgg
     2941 ctccccgagc tcgtcccagg cccggtcgac gtcgtcggcc acgaacaccg tcatcggcgc
     3001 ccccgggttg ggcatgacgc acccccgtgg ctcgatgccg gcggctgcgc actcctgctc
     3061 gtagacctgc gccaggccgt cgacgttggt ctgggcgaac agaccgaggc cccggcgggc
     3121 ggcccgacgc gccgcgggcc ggctggcccc accccagaac agggtgatcc ggccctcggg
     3181 ggcccgcccg atcgatccgg gtcgaccgtg gggggtcagg tcgcggcccg ccagcgcgtc
     3241 gaacagggtg tcgaggtgct cgtcggcgag ccggccccgg cgttcgaagt cgacgccgag
     3301 cagggcgtac tcctcgggcc ggtagcccac gccgagcacc cagccgaccc gaccgccgct
     3361 gatgtggtcg atcaccgcca tctcctcggc cagtcggacc gggtcgtaca ggggcaggat
     3421 cgccgcggtg accatgaagt acgtcgaggt ggtccgggcg gccatcgccg cgaccatggt
     3481 ccccggcgag gtcaggtacc cgtcggccac cgcgtggtgc tcagagacgg tcacgaacgg
     3541 cgcgaaggac ccctcggacc actcgaccat gtcgagcgcg gcacggtagg cgccgcggac
     3601 gtcctggccg ggtgccagcc gcatgtcgaa cctcaacccg atcacggggc cgaaccgtag
     3661 ccaccgcggc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcgccag
     3781 cgccccgacg aacttgtagc cccaagtcgc acccgtgacc gcccagctac aacatcgcca
     3841 gcgccccacg aacttgtagc ccccggtcgc tccggtgacc gcccagctac aacatcgcca
     3901 gcgcccgacg aacttgtagc cccaggtcgc tccggtgacc gcccagcgac aacatcacca
     3961 gcgccccacg aacttgtagc ccccggtcgc tcccgtgacc gcccagcgac aagttcggtg
     4021 ggccgcgtcg gccagactgt cggtcgaccc agcagcatcg agggggacgt cggagtgggt
     4081 ttcctgcaac cgaacctgcc ggtggtcgac cacgaggtct gggaccacct gccccgccag
     4141 gagcggatcg tgccgatgac gcggcacttc gcgctgcacg ggttcggctc ccccgacgtc
     4201 gtcatcctct cctacatcgt caagatcggg atctacctcg ccgtcggctg ggcgttcgtg
     4261 atgaccaccc ccggcatcga cggggcgttc gacgtcgggg cctggtggtc caacccggcg
     4321 gtcttcttca agttcgcgtt ctggacgatg ctgttcgagg tgctgggcct gggctgcggg
     4381 ttcggtccgc tcaacctgcg cttcatcccg ccgctcggct cgttcctgta ctggctgcgg
     4441 ccgggcacga tccgcctgcc accgtggccg cggcgcaacc cgatcgccgg cggcgacacc
     4501 cgcaccccgt tcgacgtcgc gctctacgcc gggctgctcg tcgccctcgt cgtcaccctc
     4561 gtcggggcga tcccccgatg ggaggtgctg accgtcgtgg tgctgctggg gttcatcggc
     4621 ctgcgcgacc agaccgtgtt cctggccgcg cggggcgagg tgtacggatc gatcgccctc
     4681 gccgcgctgt tcagcgccgg cgaccaggtc accgcggcca agttcgtgat ggtgatcatc
     4741 tggttcggcg ccgcggtgtc gaagctcaac cggcacttcc cgttcgtggt cgccgccatg
     4801 atggccaaca gcccggtgct gcgctcgacc cggctcaagg cccacctgtg gcgcgacgca
     4861 cccgatgacc tgcgaccggg acggcccgcg gcggtgctcg cccacaccgg gacggtcgtc
     4921 gagctggtcg caccgatggt cctgctgctc agcggcggcg ggtgggtcac cacggttgcc
     4981 gcgctggtga tgatcgggtt ccacctgacg atcctcacca gcttcccgat gggcgtccca
     5041 ctcgagtgga acgtgttcat gatcatcggg atcgtcgtgc tgttcctcgg caaccccgga
     5101 ctggacctgg gcgcgctgtc gcagccggtc gctctggcgg cgctggtcct cgggctgctg
     5161 gccgtcgtcg tctacgggaa cctggtgccc gagcaggtgt cgttcctgcc atcgatgcgc
     5221 tactacgccg gcaactgggc ggcgaccatg tggtgcttcc gcggcgacgc cctcgagcgg
     5281 ttcgagcgca acgtgaccac cgcgaccctg ctcccgcacc aacagctgga gaaggtctac
     5341 ggcagcgccg aggaggcggc cgtgccgctc cacatgggct acgccttccg cgggttccac
     5401 acccacggcc gggcgctgtg gaccctggtg gccgaggcgt gcgggcccga ccacgaggac
     5461 tacctggtgc tcgacggcga gctggtggcg ggcaccgctc tcgggtggaa cttcggcgac
     5521 ggccacctcc acgacgagca gctgatcgag gccctgcagc gccgctgcga cttcgcgccc
     5581 ggcgacgtgc ggatcgtctt cctcgagtcc caaccgttcc accgcggcac ccagcagtac
     5641 cgcctgatcg acgctgccac cggtccgatc ggtcggggcc acgtggtcgt caccgacatg
     5701 gtcgcccgcc aacccactga cgccgacctg ccgctgcacc gcgaccccgg caccgagccg
     5761 tgaccggcga cggcgatatg accgacgacg acgatgtgac cgacgacggc gatgtgaccg
     5821 acagcggtga cgtgaccggg ccgggcaccc gccgacggcg ggcccgggcg ctgcgcgagt
     5881 tcctcgacac cgaggtcgcc ggggccgtcg tcctgctcgt cgcggtcgtc gtggcgttgg
     5941 tgtgggccaa ctcgtccttg tcgagcagct actccgacct gtggcacacc gagctgtcgg
     6001 tgagcgtggg ctggggtctg gacatgtcga tgcaccaggt ggtcaacgac ggcctgatga
     6061 ccgtgttctt cttcgtcgtc ggcctcgaga tcaagcggga gctggtcgac ggcgagttgt
     6121 cggacccccg cacggccgcg gtgcccgtgg ccgcggcgat cggcggcatg gtcgtcccgg
     6181 cgctgctgtt cgtggcgatc aacgcctcga ccggcaccac ccgggggtgg ggcatcccga
     6241 tggcgaccga catcgccttc gccctcggcg tcctcgcggt cgcggggtcc cggttggcgt
     6301 cgggcgccaa gctgttcctg ctcacctcgg cggtggtcga cgacatcggc gcgatcgtgg
     6361 tgatcgcggt ggtgtactcg acgtcgatct cgcccggcgc cctgctcggc gccgcggtgg
     6421 tgatcggcat ggttctgctc gcccgggcca caggggtgca gagcctggtc gtctacgtgc
     6481 tgctcggcgt cctgttgtgg gacgccatgt tcgagtcggg ggtgcacgcc accatcgccg
     6541 gggtcgtgct cgggctgatc acccccgccc gaggacccga cagcgttgcc gagcgactcg
     6601 agcaccgcct gcacccgctg agcagcttcg tgatcgtccc gctgttcgcc ctcgccaacg
     6661 ccggggtgcg cctggacgtc gggtccctgt cggaaccggc gccgcgggcc gtcgcgctcg
     6721 gggtggtggt cggcctggtc atcggaaaga ccgtcggcat ctcggccgcg accgcgatcg
     6781 cggtcaagac ccggatcggc cgtctgcccg acggggtcgg agccggcgag gtgatcggca
     6841 tggcgatggt ggccgggatc ggcttcaccg tgtcgctgtt cgtcaccgag ctcgccttcg
     6901 acgacccggc catccgcgac gcggccaaga ccgcggtgct ggtcgcctcg gtggtcgccg
     6961 cggtcgccgg cggaacgacg ctgtggtggc gtggccgacg gaccgcggag tctccccaac
     7021 aggcccggac cgtccccgac cgctgattcc cgggcggctt gaaccaccct gggttcgttg
     7081 gaggctcgtt ctattggttt ccaaccccga cggggtcggc ttgttgggca gcgtagaacg
     7141 ctgcttcgaa ctctgccggg ggcacgtggc cgcagtggct gtggagccgc tgttcgttga
     7201 accagtacac ccagtcgagc gtcgcgtgtt cgagctcgtt gacgtcggcc cagcctgtgg
     7261 tgtcgggccc gtagacgcac tcagctttgt agagaccgtt ggttgtctcg gccagggcgt
     7321 tgtcgtagga gtcggcgacg gtgccgatcg aagggcgggc tccgatctcg tcgagccgtt
     7381 cggtgaaccg cacactggtg aactggctgc cggcgtcgga gtgggtgacg agcccgacga
     7441 ggcgccctcc gcctctggat cgtctggcca tctcgacggc gtcgaggacc atgtcggtac
     7501 gcatgtgcga cgcggcgcgc cagccgacga tccgacgact gaacgcgtcg acgatgaaac
     7561 acacgtacgc catcccggac cgggtcggca cgtaggtgta ggggtcggac cggagcgccc
     7621 tgtcagcctt tcggctggtg ggtttctccg gcccgccctc cgaaccggac gtgcgacttc
     7681 caccgcatcc g
//
LOCUS       DAOQOD010000147         7611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3622_length_7611_cov_9.788645, whole genome shotgun sequence.
ACCESSION   DAOQOD010000147 DAOQOD010000000
VERSION     DAOQOD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3622_length_7611_cov_9.788645"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1522
                     /gene="murC"
                     /locus_tag="PLZ72_12545"
     CDS             95..1522
                     /gene="murC"
                     /locus_tag="PLZ72_12545"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNA37172.1"
                     /translation="MDGPSLDLSVPRHIHIVAIGGAGMSGIATLLAQSGHIVTGSDAV
                     DSPTLARLADLGVAVWVGHDPDRVAALDPLDAVGISSAVGDDDPEVLAALRRGVPVLR
                     RTALLPALAAREPLLSVAGTHGKTTTTSLLVAALRGAGEDPSFLTGAPVAALGGVSAA
                     WRPGRHLVLEADESDGSFLSAPRVGAIVTNVEPDHLEFWGGWDELRSGFRQFLSGTDG
                     PRVVCADDPVAADLAAPLGAVTYGTAEGADHRVADLVLGADGSRFTLVGTARAGRYSG
                     SGGGGVSVELPLPGRHNALDAAAALALVDELGLDVTAAAAALADFGGVARRFERKGTA
                     AGVTVVDDYAHLPTEVRAALAAGASGGYERVVAVFQPHRYSRTQALWEEFGACFGDAD
                     LLVLTDIYPAGEEPRPGVTGRLLVDSTLAADPGRSLLWAPTLDDVVEQLVDVLAPGDL
                     LMTVGAGDVTTVGPRVLERLTQERR"
     gene            1519..2481
                     /gene="murB"
                     /locus_tag="PLZ72_12550"
     CDS             1519..2481
                     /gene="murB"
                     /locus_tag="PLZ72_12550"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNA37173.1"
                     /translation="MSPPTTPWVEHAPIGALSTYRVGGAARWLVVAHRIEDLHDVVAR
                     RGDLPVLVVGKGSNLLVGDAGFDGIAVQLGEAFAAIEVLGAAGPDSTGGGAATEPEDA
                     AGVIVRAGAAASLPVVARRSVAAGLTGFEWAVGVPGSIGGAVRMNAGGHGADMAASLV
                     GVRLIDLRTGEDEVVRSAELDLAYRSSSVRADQVVLHADLRLRPGDRTRSEELLSEIV
                     RWRREHQPGGANAGSVFTNPPGDSAGRLIDAAGCRGMRVGTAEVSTKHANFIQADAGG
                     SADDVFALMVAVAREVRRVHGVDLHPETRMVGLPPFADAVEADL"
     gene            2478..3578
                     /locus_tag="PLZ72_12555"
     CDS             2478..3578
                     /locus_tag="PLZ72_12555"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNA37174.1"
                     /translation="MTDTLSPPRRASRERSDRGRLRSGDATPRVVDAEPGEDPRIRDR
                     RRSVQADRRRRRRAVLFVALAVVVLVASAAALSRSAWFDVDRIVVDGPDGIDRDELRQ
                     ASGIDRGDAMVDVDLAAARRSIMALPSVASARVEREWPGTIRVVFHAESPLAVLAGGE
                     RRVLIGRGGRVLAELAQDDPTPEGLPTVTVEDPSAVSELEVGSALPESLSSVVVVLEQ
                     LPEPLRSRSAGVTLDAGGNLSMALRADPAVDGSDGTVELGPADELASKLLAAASIVAG
                     ARMECLDVLDVREPSRPTISRDRGCDPGPPTVGATTVPARTEPDGNARTADPRSGRTS
                     TSTTTATRRTAAVSTTTAPGSTRRGSEPGAPG"
     gene            3673..4782
                     /gene="ftsZ"
                     /locus_tag="PLZ72_12560"
     CDS             3673..4782
                     /gene="ftsZ"
                     /locus_tag="PLZ72_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNA37175.1"
                     /translation="MAHNPQNYLAVIKVVGIGGGGCNAVNRMIDAGLKGVEFIAINTD
                     AQALLMSDADIKLDIGRELTRGLGAGSDPDVGRQAAEDHRAEIEEVLRGADMVFVTAG
                     EGGGTGTGAAPVVAEIGKSQGALTIAVVTRPFGFEGRRRSVQADQGISKLKEKVDTQI
                     VIPNDRLLTVANDKTSVLNAFKMADEVLLQGVQGITDLITTPGLINTDFADVRMIMND
                     AGSALMGVGYATGEGRALNAARQAISSPLLEAAIDGARGILLNISGGSDLGLFEVNEA
                     AQVIHEVAHPEANIIFGAVIDDELGDEVRITVIAAGFERWDESKGSRRPTTSAGGPPR
                     SEDRPSSIGDVFGGDEEDDELFGGDDDFDVPSFLK"
     gene            4808..5569
                     /locus_tag="PLZ72_12565"
     CDS             4808..5569
                     /locus_tag="PLZ72_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNA37176.1"
                     /translation="MTRREVLHSDGLPSVHMAFTDAAVGSLRVPEVGFSSSAELEQRR
                     RRVAPHPWTWLRQVHGDRVVEVDAPGEWTGAEADAAVTTTLGAVLAVHTADCAGVVLV
                     GDGADPVTGEPVRVIGAAHAGWRGLAAGVVEGTVEHMRRMGADRFDWDLGPCISPAAY
                     AFGAGELDELCERLGPELAATTLDGEPALDLRAGVRAALARAGVDSGVGTGPAVVPCT
                     ALDDGFFSWRARQDEGRQAAVVWLAPDDHDYPWHP"
     gene            5557..6243
                     /locus_tag="PLZ72_12570"
     CDS             5557..6243
                     /locus_tag="PLZ72_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNA37177.1"
                     /translation="MAPLNEPPPAPPQRALVEQIAERGAQLRSRIAELTDRDVAVVCV
                     TKGHPVEVAAAAVAAGFTRLGENYAQELRDKAAALIGPTPEWHFIGRLQTNKVRLVDR
                     VVSLWQSVDRAALADRIAQRVPGAAVLVQVDLAGLAGRGGCPPEDVPALVEHCRGRGL
                     DVRGLMGVGPPGPAEEARPGFRLLTSIADDLGLEERSMGMSDDLDVAIEAGSTMVRVG
                     SALVGPRAPR"
     gene            6367..6969
                     /locus_tag="PLZ72_12575"
     CDS             6367..6969
                     /locus_tag="PLZ72_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNA37178.1"
                     /translation="MKNARSWLGLGQDPYYDAEYADPPLDDEWEDDVDDEEVVDVYPT
                     AAAPRSRPAAAPRRSANGGAGTVRSVPTPAADEGDGDGGVRVISSAPGSDADSGRGVV
                     RPLPGVSRPHVVNPAGFNDVQEVADTFKRNQPVILNLQGVDRDLSRRLIDFGSGLCYG
                     LEGNMERVTDQVFLLTPAGAQVSDDDRRRIREGGLDREDR"
     gene            7000..7260
                     /locus_tag="PLZ72_12580"
     CDS             7000..7260
                     /locus_tag="PLZ72_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNA37179.1"
                     /translation="MIVVAWILNALLILLVLRAVLSWFPPGGSFMEQVNGFLVVCTEW
                     MLAPVRRVVPPLRLGGAMLDMSFMVVFVVIMVLQNVVAGLAR"
     gene            7338..>7611
                     /locus_tag="PLZ72_12585"
     CDS             7338..>7611
                     /locus_tag="PLZ72_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37180.1"
                     /translation="MDLTPELFEKTEFTERRKGYDIEQVETFMEDAGTALAQLLVRVR
                     HTEERASVAEGRLADAEARAASAEEKAGEVQARAQAAVAEAQANAAT"
BASE COUNT          968 a         2778 c         2826 g         1039 t
ORIGIN      
        1 gacgccgccc gctccctcgg tcggcccgac gccgccgccg ccgtcgccga cctggtcgag
       61 acccatgccc gcccccggta ggaacggcgc cgacgtggac ggaccgtccc tggacctgtc
      121 ggtgccacgc cacatccaca tcgtggcgat cggcggagcc gggatgagcg ggatcgccac
      181 gctgctggcg cagtcgggtc acatcgtcac cggcagcgac gccgtcgact cacccaccct
      241 ggcccgtctg gccgacctcg gcgtcgccgt gtgggtcggt cacgaccccg atcgggtcgc
      301 ggccctggac ccgctcgacg cggtcggcat cagctccgca gtgggagacg acgaccccga
      361 ggtcctggcc gcgctccgac gcggcgtgcc ggtcctgcgg cgcaccgccc tgctgccggc
      421 cctggccgcc cgggagccgc tgctgtcggt cgccggcacg cacggcaaga ccaccaccac
      481 gtcgctgctg gtcgccgcgt tgcggggagc gggggaggac ccctcgttcc tcaccggcgc
      541 cccggtcgcc gccctcggcg gcgtgtccgc cgcgtggcgg ccgggccggc acctggttct
      601 cgaggccgac gagagcgacg ggtcgttcct gtccgctccc cgcgtcgggg cgatcgtcac
      661 caacgtggag ccggaccacc tcgagttctg gggcggttgg gacgagctcc gctccgggtt
      721 ccgccagttc ctgtcgggca ccgacggacc ccgggtggtg tgcgcggacg acccggtcgc
      781 cgccgatctg gccgccccgc tgggcgcggt cacctacggc accgccgagg gggccgatca
      841 tcgggtggcc gatctggtcc tcggagccga cgggagccgg ttcacgctcg tcgggaccgc
      901 ccgcgccggg cggtacagcg gatccggagg tggcggcgtg tcggtcgagc tgccgcttcc
      961 tggacgtcac aacgcgctcg acgccgcggc cgcgctcgcc ctggtcgacg agctcggcct
     1021 cgacgtgacc gccgccgcgg ccgccctggc cgacttcggc ggcgtggccc gacgcttcga
     1081 gcgcaagggc accgcggcgg gcgtcaccgt ggtcgacgac tacgcccacc tgcccaccga
     1141 ggtccgcgcc gcgctcgccg cgggagccag cggcggctac gagcgggtgg tggcggtgtt
     1201 ccaacctcac cgctactcgc gcacccaggc gctgtgggag gagttcggcg cctgcttcgg
     1261 cgacgccgac ctccttgtgc tgaccgacat ctacccggcg ggggaggagc cgaggcccgg
     1321 ggtgacgggc aggctgctgg tcgactccac cctggcggcg gatccgggcc ggtcgttgct
     1381 gtgggccccg accctcgacg acgtcgtcga gcagctggtc gacgtgctgg ccccggggga
     1441 cctgctgatg accgtcggag cgggagacgt caccaccgtc ggcccccggg tgctcgaacg
     1501 tctgacgcag gagcgccggt gagcccgccg acgacgccgt gggtggagca cgctccgatc
     1561 ggggcgctga gcacctaccg ggtcggcggc gccgcccggt ggctggtggt cgcccaccgg
     1621 atcgaggacc tgcacgacgt cgtggcgcgg cggggcgacc tcccggtcct ggtggtcggc
     1681 aaggggtcca acctgctcgt cggcgacgcc ggcttcgacg ggatcgccgt gcagctcggc
     1741 gaggccttcg ccgccatcga ggtgctcgga gcggccgggc ccgacagcac cggtggcggc
     1801 gccgcgaccg aaccggagga tgccgcgggt gtgatcgtgc gcgcgggcgc cgccgcatcc
     1861 ctgcctgtcg tggcacgccg cagcgtcgcg gcgggtctga ccggcttcga gtgggccgtc
     1921 ggagtgccgg gctcgatcgg gggtgccgtg cgcatgaacg caggtggtca cggcgccgac
     1981 atggccgcgt ctctggtggg ggtccgcctc atcgacctgc ggaccgggga ggatgaggtg
     2041 gtgcgcagcg ccgagctcga cctggcctac cggagctcca gcgtgcgcgc cgatcaggtc
     2101 gtgctccacg ccgatctccg gctgaggccg ggagatcgga cccgcagcga ggagctgctg
     2161 tcggagatcg tgcggtggcg gcgcgagcac cagcccggag gagcgaacgc cggatcggtg
     2221 ttcaccaacc ccccgggcga ctcggccgga cggttgatcg acgccgccgg ctgccgcggc
     2281 atgagggtcg gcaccgccga ggtctccacc aagcacgcca acttcatcca ggccgatgcc
     2341 ggcggatccg ccgacgacgt gttcgccctg atggtcgcgg tggcccggga ggtgcgtcgg
     2401 gtccacggcg tcgacctgca ccccgagacg cgcatggtcg ggctcccccc gttcgccgac
     2461 gcggtcgagg cggacctgtg accgacacgc tgagcccgcc gcgccgagcg agccgggagc
     2521 ggtccgatcg gggccggctc cggagcggcg acgccacccc ccgggtcgtc gacgccgagc
     2581 cgggcgagga cccgcgcatc cgggatcggc gccggtcggt ccaggccgac cgccgccgac
     2641 gtcggcgggc cgtgctgttc gtcgccctgg ccgtggtggt cctggtcgcg tccgcggcag
     2701 cgctcagccg gtcggcgtgg ttcgacgtcg accgcatcgt cgtcgacggg cccgacggaa
     2761 tcgaccgcga cgagctgcga caggcgtcgg gcatcgaccg gggcgacgcg atggtcgacg
     2821 tcgacctggc cgcggcgcgt cgttcgatca tggcgttgcc gtcggtggcc tcggcgcgcg
     2881 tcgagcgcga gtggcccggc acgatccgcg tcgtgttcca cgccgagtcc ccgttggcgg
     2941 tgctcgccgg cggggagcgt cgcgtcctga tcggccgggg cggccgggtc ctggccgagc
     3001 tcgcgcagga cgatcccaca cccgaaggtc tgccgacggt caccgtcgag gacccttccg
     3061 ccgtctccga gctcgaggtc ggctcggccc tccccgagag cctgtcgtcg gtggtggtcg
     3121 tgctcgagca gctcccggag ccgctgcgct cccggtccgc cggtgtcacc ctcgacgccg
     3181 ggggcaacct gtcgatggcg ctccgggccg atccggcggt cgacggatcc gacggcaccg
     3241 tcgagctcgg cccggccgac gagctggcct ccaagctgct ggcggccgcg tcgatcgtcg
     3301 ccggcgcgag gatggagtgc ctggacgtcc tcgacgtgcg agagccgtcg cggccgacca
     3361 tcagccggga ccggggatgt gacccgggcc cgccgacggt gggcgccacg accgtgccgg
     3421 cccggaccga gcccgacggg aacgcccgga ccgctgatcc gcgctccgga cggacctcga
     3481 catcgacgac gaccgcgacg cgtcggaccg ccgcggtgtc gaccacgacg gctcccgggt
     3541 cgacccgacg aggatccgag ccgggagcgc cggggtgagc atcgttgccc cggtgcctcg
     3601 gccggccgta ttgtcacgtg ccgcatcgcc gggccgagat cgcgccccgg ttcctggagg
     3661 tgtctgagac cgatggccca caacccgcag aactacctcg ccgtcatcaa ggtggtcggc
     3721 atcggtggag gtggctgcaa cgcggtcaac cggatgatcg atgccgggct caagggcgtc
     3781 gagttcatcg ccatcaacac cgacgcccag gcgttgctga tgagcgacgc cgacatcaag
     3841 ctcgacatcg gtcgtgagct gacccggggc ctcggcgccg gctcggaccc cgacgtcggc
     3901 cgccaggccg ccgaggacca ccgcgccgag atcgaagagg tcctccgcgg cgccgacatg
     3961 gtgttcgtca ccgccggcga gggcggcggc accggcaccg gcgccgctcc ggtggtcgcc
     4021 gagatcggca agtcgcaggg tgcgttgacc atcgcggtgg tcacccgacc gttcggattc
     4081 gaggggcgtc gccgctcggt gcaggccgac cagggcatct ccaagctcaa ggagaaggtc
     4141 gacacccaga tcgtcatccc caacgaccga ctgctcaccg tcgccaacga caagacctcg
     4201 gtgctcaacg cgttcaagat ggccgacgag gtgctgctgc agggcgtcca gggcatcacc
     4261 gacctcatca cgaccccggg tctgatcaac accgacttcg ccgatgtccg gatgatcatg
     4321 aacgacgccg gctccgccct catgggcgtc ggctacgcca ccggcgaggg ccgggcgctc
     4381 aacgctgcgc gtcaggccat ctcgtcgccg ctgctcgagg ccgccatcga cggtgcccgc
     4441 gggatcctgc tcaacatctc cggcggatcg gacctcggcc tgttcgaggt gaacgaagca
     4501 gcccaggtca tccacgaggt cgcgcacccc gaggccaaca tcatcttcgg cgccgtcatc
     4561 gacgacgagc tcggcgacga ggtccgcatc acggtgatcg cggcgggctt cgagcgctgg
     4621 gacgagtcca agggcagtcg tcgaccgacc acgtcggccg gcggacctcc ccggagcgag
     4681 gaccgcccgt cgtcgatcgg cgacgtgttc ggcggcgacg aggaggacga cgagctgttc
     4741 ggcggcgacg acgacttcga cgtcccgtcc ttcctcaagt gagctccggg gcccgaccgc
     4801 cggtgtcgtg acccgacgcg aggtcctcca ctccgacggt ctgccgtcgg tgcacatggc
     4861 gttcaccgac gccgcggtcg gatcgctgcg cgttcccgag gtgggcttct cctcctccgc
     4921 cgagctcgag cagcgccgcc gacgcgtggc gccgcacccg tggacgtggc tgcgccaggt
     4981 ccacggcgac cgggtggtcg aggtggatgc cccgggggag tggaccgggg ccgaagccga
     5041 cgccgcggtc acgaccactc tgggggcggt gctggcggtg cacaccgccg actgcgccgg
     5101 ggtcgtcctg gtcggcgacg gcgccgaccc ggtgaccggg gagccggtgc gggtcatcgg
     5161 cgccgcgcac gccgggtgga ggggactggc cgccggcgtg gtcgaaggca ccgtcgagca
     5221 catgcgccgg atgggtgccg acaggttcga ctgggacctg ggaccgtgca tctcgccggc
     5281 cgcctacgcc ttcggcgcag gcgagctcga cgagctgtgc gaacggctcg gccccgagct
     5341 ggcggccacc acgctcgacg gcgagccggc cctcgacctg cgggccgggg tgcgcgccgc
     5401 tctggcccgg gcaggggtgg acagcggggt ggggacggga ccggccgtgg tgccgtgcac
     5461 cgctctcgac gacggcttct tctcgtggag ggcccggcag gacgagggcc gccaggcggc
     5521 ggtggtgtgg ctggcccccg acgaccacga ctacccgtgg cacccctgaa cgagcccccg
     5581 ccggcgccgc cgcagcgtgc gctggtcgaa cagatcgcgg agcggggtgc gcagctgcgc
     5641 agccggatcg ccgagctcac cgaccgcgac gtcgccgtgg tctgcgtcac caagggccac
     5701 cccgtcgagg tcgccgccgc cgcggtcgcc gcgggcttca cccgcctggg cgagaactac
     5761 gcccaggagc tgcgcgacaa ggccgcggcg ctcatcggtc caaccccgga gtggcacttc
     5821 atcggacggc tgcagaccaa caaggtccgg ctcgtcgatc gggtcgtgtc gttgtggcag
     5881 tcggtcgacc gcgccgctct ggccgatcgc atcgcccagc gggttcccgg cgccgccgtg
     5941 ctcgtgcagg tggacctggc cggactggcg ggccggggtg ggtgtccacc cgaggacgtt
     6001 cccgcgctgg tcgagcactg ccggggtcgg ggtctcgacg tgcgcggcct gatgggtgtc
     6061 gggccgcccg gcccggccga ggaggcccgg cccgggttcc gtctgctcac ctcgatcgcg
     6121 gacgacctcg gactcgagga gcggtcgatg gggatgtccg acgacctcga cgtcgccatc
     6181 gaggcggggt cgaccatggt gcgcgtgggg tccgccctgg tgggtccccg tgccccgcgt
     6241 tgagccggtg gacctccgac cgggctcccg gagtcggtcc agggggtcgc agccccaccg
     6301 ggcgtccgtc ccgccgggtc tgacgccact acactccgcg gccgaggagc gaggatgtcg
     6361 aacttcatga agaacgcacg ctcgtggctc gggctggggc aggaccccta ctacgacgcc
     6421 gagtacgccg atcccccgct cgacgacgag tgggaggacg acgtggacga cgaggaggtc
     6481 gtcgacgtgt accccaccgc ggccgcgccg cgctcccgtc cggccgccgc tccgcgccgg
     6541 agcgccaacg gaggggccgg caccgtgcgg tccgtcccca ccccggccgc cgatgagggc
     6601 gacggtgacg gcggggtccg cgtgatctcg tccgccccgg gctccgacgc ggattccggg
     6661 cggggtgtcg ttcgtccgct ccccggtgtc agccggcccc acgtcgtgaa ccccgccggg
     6721 ttcaacgacg tccaggaggt ggccgacacc ttcaagcgca accagccggt gatcctcaac
     6781 ctccagggcg tcgaccgcga cctgtcgcgg cgtctcatcg acttcggcag cggcctctgc
     6841 tacgggctcg aggggaacat ggagcgcgtc accgaccagg tgttcctgtt gaccccggcc
     6901 ggcgcccagg tctccgacga cgaccgtcgg cggatccgcg aaggcgggct ggaccgcgag
     6961 gatcgctgag ccggtcttcc cccgacccgg accaacccca tgatcgtcgt cgcctggatc
     7021 ctcaacgccc tgctcatcct gctcgtcctg cgggcggtgc tgagctggtt cccgcccggc
     7081 ggctccttca tggagcaggt caacggcttc ctggtggtct gcaccgagtg gatgctcgcc
     7141 cccgtgcgcc gggtcgtccc gcccctgcgg ctgggaggcg ccatgctcga catgtcgttc
     7201 atggtcgtct tcgtcgtgat catggtcctg cagaacgtgg tggccggcct cgcccggtga
     7261 ggtcggccgc ggcgcagtcg ccgtcgagcc cgtgacggtt cggtgacgga cgaccgctga
     7321 cgactagcat cgccggcatg gatctcacgc cggagctgtt cgagaagacc gagttcaccg
     7381 agcgtcgcaa gggctacgac atcgagcagg tcgagacgtt catggaggac gccggcaccg
     7441 ccctggccca gctgctggtg cgcgtccgtc acaccgagga gcgggcgtcg gtcgccgagg
     7501 gccgcctggc cgacgccgag gcgcgggccg cgtcggccga ggagaaggcc ggtgaggtgc
     7561 aggcccgtgc ccaggccgcc gtcgccgagg cccaggccaa cgcggcgacc g
//
LOCUS       DAOQOD010000148         7541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3677_length_7541_cov_11.136121, whole genome shotgun sequence.
ACCESSION   DAOQOD010000148 DAOQOD010000000
VERSION     DAOQOD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_7541_cov_11.136121"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLZ72_12590"
     CDS             complement(<1..477)
                     /locus_tag="PLZ72_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37181.1"
                     /translation="MKGSSGSSTSRKAVADGSAASPSNSPRRGIARLAVRPSVEGPKV
                     RLGILWFFVALAAVTAGRGPTAVLWGLLAALAARELLVLWWPIAASAGGAPSPRPADG
                     AASGAPGGLVLGAVVLAVRDSRLALLACYARARAGSPTLTASPRVRMFVSGVGAVTF"
     gene            complement(719..955)
                     /locus_tag="PLZ72_12595"
     CDS             complement(719..955)
                     /locus_tag="PLZ72_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNA37182.1"
                     /translation="MPVVRLFAQAREAAGTATDVVAGDTVAAVLDHARERWGEGFSAV
                     LGTCGVWVNGEPAELTDAVGADDEVAVLPPVSGG"
     gene            1026..1610
                     /locus_tag="PLZ72_12600"
     CDS             1026..1610
                     /locus_tag="PLZ72_12600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNA37183.1"
                     /translation="MTQLLVIRHGQSEWNADGRWQGQENPPLTNLGRQQARTAARAVG
                     AIDAIYASPLDRAATTASILSDELGIGPVVTLTGLMERHAGEWQGLTRDEIEEDFPGY
                     LDSGRRPPGWEPDEAVEARVLGAFDAIATQHPYGTVVAVAHAGVIFAAESALGGTWER
                     LANLGGRWLEHTDAGWRIGDRVHLLIDETIPDQI"
     gene            complement(1640..2029)
                     /locus_tag="PLZ72_12605"
     CDS             complement(1640..2029)
                     /locus_tag="PLZ72_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37184.1"
                     /translation="MESDPITPDAADRPDARPPAAPVPAEAVGGHGLAADGMAADGMA
                     ADETASDETAGDHGTAADELVLLTSIEEDLAAVDAAMAALDGIDTTTMDGATAAAQVE
                     AIAGRDRFDPTDPTAPTAPAARTAPTV"
     gene            2074..2559
                     /locus_tag="PLZ72_12610"
     CDS             2074..2559
                     /locus_tag="PLZ72_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37185.1"
                     /translation="MTPSTLPALAAASSPSSTQVAVVVADVQGSVLVRSFLIAVVVMA
                     PVWVLVRKRVTRSHATDDSGEVDPDADAAPRLEDVIAEVSRLAPEVRATGSATLTVPA
                     DVTVDGRAVDSGTVDALVRDSLRRSGLVAAAEIDTPQGRTLELRPLGPGPDADPDRTR
                     T"
     gene            2568..2849
                     /locus_tag="PLZ72_12615"
     CDS             2568..2849
                     /locus_tag="PLZ72_12615"
                     /inference="COORDINATES: protein motif:HMM:NF022683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HNA37186.1"
                     /translation="MTTGPATSTVTLTDEEQETEDGPVAHIVRTLPGEDAAAKVLAAR
                     VEGTPLEALCGHVWVPSRDPKQLPVCQKCKDIYGMYRDFNDGLDDTPRM"
     gene            2929..3894
                     /locus_tag="PLZ72_12620"
     CDS             2929..3894
                     /locus_tag="PLZ72_12620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA37187.1"
                     /translation="MRAVITGASGFLGAHLARTLKDRGVEVRAADRSRGPALDDVDVE
                     FVEIDVLDPASLRAAFEGQDRVFHLAAIISIVGDPTGEVWRVNVHGPRNAATVALECG
                     VGRFVHCSSVHSFDLERCGPSLDESGLPTTHPDAPAYDRSKRAGEEAVRLVVQKGLDA
                     VVVNPTGIIGPDDRGPSRMGETVLQFMTRKIPVGITGQFDFVDVRDVVEGLIAAGDRG
                     RTGENYLLSGSRLSIRELGMLVERYSGVPVPPLSIPLRLVTPLAPLVMQIEQRTKKPI
                     FTPDALHALKYSPVVSHYRAATELGYRSRPIHETVRDTVAWFESN"
     gene            complement(3947..5434)
                     /locus_tag="PLZ72_12625"
     CDS             complement(3947..5434)
                     /locus_tag="PLZ72_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37188.1"
                     /translation="MSATLDPRTPVLVGVGAAHRPADGSGAVEPLELMVEAALAAADD
                     AGAARLLPDCGLVAVPEGSWSYPDVARLVADRVGARRARTVRADIGVPQTQPVAVALD
                     RIRRGALDVALVVGGEAMASSRAAARAGTPIDETAQDGATPDERWSPAGEIMAAAEVA
                     AGMWAAVEQYACIENALGHAEGQPFDSQLDDIAELWAAYNAVATTNPTAAFAAPWTAA
                     ELRDPGPGNRAIASPYNKWHVSQWVVDQAAALILCSVDAARAAGVPAERWVFPHACVD
                     SSHMVSLSRRSEIHRWPAMGVVGRAVAEHIGRPLRDIEIQEIYSCFPVAVRLQQRELD
                     LDRDQRTAVAPTVTGGMAFAGGPLNNFTYQATVEVVRHLREHPEHLAMVTTVSGLLTK
                     PGITVWGGRPAPDVLIEDVAEPAAARTGVRDVTGEAQGPGTVVTATATYLDGRPVRAF
                     VIADLDDGRRWVGTCDDAGFAEATDRRATIGTIVDVTGAGCVPRS"
     gene            complement(5484..6425)
                     /locus_tag="PLZ72_12630"
     CDS             complement(5484..6425)
                     /locus_tag="PLZ72_12630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNA37189.1"
                     /translation="MPITGAMSALGHRPRPRPFVLLPVLAVGLVSFLAACVPAPPRPP
                     APVGPAEREMVFPVAGRTSYSDTFGAPRSGGRTHQGQDVMAARHTPLVAAADGTVTSL
                     TWSNSGLSGNSLTITDADGWRYVYIHLNNDAPGTDDGSNVYERAFADGIAKGQKVKAG
                     EVVGYVGDSGNAESTSPHLHFELRRPDGTVVNAYSSLRAARTLVRTDAERIADSPTGT
                     LDMVHGGAGTVSVDGWALDAHDDDPVKVSVYVAGNPVATATASVDRPDVDAAFGRGAE
                     HGFHVSDVPAAPGTTVCVVAHSLGGGGSTRIGCGVAP"
     gene            6505..7377
                     /locus_tag="PLZ72_12635"
     CDS             6505..7377
                     /locus_tag="PLZ72_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNA37190.1"
                     /translation="MTDAVLVDTPAEGVRRITLNRPDKRNALNHAVRGGVLDALREAD
                     EDPEVRVSIIRGAGSCFSAGYELGDGNEGHDLPYFTPGGEGFWPRHVTDAWMSIWDLA
                     KPVIAQVHGWCLAGGSELATGCDLVYVSDDARIGYPAVRFGVPDMQFHAWFLGPRAAM
                     EMMLTGDWISGEDAVRLGWANRVFPADRLDDEVLAMAERIALVPPDIAQLNKRTVHRA
                     MDVMGLRTAIRSGTELCALGVHQPTFRAFLGDAGIDGAVGGERTDGGSLTEALQRRDE
                     PFGDYRTGGGTEPS"
     gene            complement(7422..7494)
                     /locus_tag="PLZ72_12640"
     tRNA            complement(7422..7494)
                     /locus_tag="PLZ72_12640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7459..7461),aa:Lys,seq:ttt)
BASE COUNT         1074 a         2699 c         2792 g          976 t
ORIGIN      
        1 gaaggtcacc gcgcccaccc cgctcacgaa catgcggacg cgagggctcg ccgtcagcgt
       61 cggagacccg gctcgcgctc gcgcgtagca agcaaggagc gcgagacggc tgtcacgcac
      121 ggcgaggacg accgccccga gcaccagtcc gccgggggcg ccggaggcgg cgccatcggc
      181 cggtctcggt gacggtgcgc cgcccgcgga cgcggcgatc ggccaccaga gcaccaggag
      241 ctcccgggcg gcgagagcgg cgagcagacc ccacagcacg gcggtcggac cccggccggc
      301 ggtgacggcc gccagagcca cgaagaacca gaggatcccg agtcggacct tgggaccctc
      361 gacggaggga cggaccgcca gtcgggcgat cccccgtcgg ggcgagttcg acggtgatgc
      421 tgccgagccg tccgcaacgg ccttccgtga cgtcgacgag cccgacgatc ccttcatcga
      481 cgacgacgct cccttcgacg acttcgtcga cgctcgcttg gacgacgccc ccttggacga
      541 ccgggactgg gcgccggatg tcgatcgggt cgagctcgcc ttcgacttcg acttcccctt
      601 cttggccgag gcggagcggt tccgcttcac cggcgccacg tccgacaccg gggtgcgcgg
      661 gcccgaccga tcggtcggcg acgtcgtgga tcccggggcg ccggaccgac ccgaacgctc
      721 agccaccgga caccgggggg agcaccgcca cctcgtcgtc cgcccccacc gcgtcggtca
      781 gctccgccgg ctcgccgttc acccacacac cgcatgtgcc caggaccgcg gagaacccct
      841 cgccccaccg ctcgcgggca tggtcgagca ccgcggccac cgtgtcaccc gccacgacgt
      901 cggtcgcggt gccggcggcc tcgcgggcct gggcgaagag gcggacgacg ggcaccgatc
      961 gagcctactc cgcgcccctc cggcggttgc cgacgacgtc ggggcaaccg atagcgtcgg
     1021 cgcccgtgac gcagctcctg gtgatccgac acggccagtc ggagtggaac gcggacggac
     1081 gctggcaggg ccaggagaac ccgccgctca ccaacctcgg ccgtcagcag gcccgcaccg
     1141 cggcccgggc cgtgggcgcc atcgacgcca tctacgcctc accgctggac cgggccgcga
     1201 ccaccgcgtc gatcctcagc gacgaactcg gcatcgggcc ggtggtgaca ctcaccggac
     1261 tgatggaacg tcacgccggc gagtggcagg ggttgacccg cgacgagatc gaggaggact
     1321 tccccggcta cctcgacagc gggcggcgtc cgccggggtg ggagcccgac gaggcggtcg
     1381 aagcccgggt gctcggagcg ttcgacgcca tcgccaccca gcacccgtac ggcacggtcg
     1441 ttgcggtcgc gcacgccggg gtgatcttcg ccgcggagtc ggcgctcgga ggcacgtggg
     1501 agcggctggc caacctgggc ggtcggtggc tggagcacac cgacgccggg tggcggatcg
     1561 gtgaccgggt ccacctgctg atcgacgaaa cgatccccga ccagatctga cccgggcccg
     1621 gccggccctg agatcggggt cagacggtcg gagcggtccg agcggccgga gcagtcggag
     1681 cagtcgggtc ggtcgggtcg aaccggtcgc gaccggcgat cgcctcgacc tgggccgcgg
     1741 cggtggcgcc gtccatcgtc gtggtgtcga tcccgtcgag ggcggccatg gcggcatcga
     1801 ccgcggccag gtcctcttcg atcgaggtga gcagcaccaa ctcgtcggca gcggttccgt
     1861 ggtcacccgc cgtctcgtca ctcgccgtct cgtcagcagc catcccgtcg gcggccatcc
     1921 cgtcggcggc aagcccgtgg ccaccgacgg cctccgcggg aaccggagca gccgggggcc
     1981 gggcgtccgg acggtcggca gcgtcggggg tgatcggatc ggactccacg gcgtgggagc
     2041 gtagcggcgc ccaccggtac gatcccgtcc ccggtgaccc cctcgaccct gcccgccctc
     2101 gccgccgcga gctctccctc ctcgacccag gtcgcggtgg tcgtcgcgga cgtccagggc
     2161 agcgtgctgg tccggtcgtt cctgatcgcc gtcgtggtga tggccccggt gtgggtgctg
     2221 gtgcgcaagc gggtgacgcg ctcccacgcc acggacgact ccggagaggt cgatccggac
     2281 gccgatgccg ccccgcgcct cgaggacgtg atcgccgagg tgtcccggct ggcacccgag
     2341 gtccgggcga ccggatcggc gaccctgacc gtgccggccg acgtgaccgt cgacggcagg
     2401 gcggtcgact ccggcaccgt ggacgccctg gttcgcgact cgctgcgtcg cagcggcctg
     2461 gtggccgcag ccgagatcga caccccgcag gggcgtaccc tcgagctacg gccactcggc
     2521 ccgggacccg acgccgaccc cgatcggaca cgcacatgac cgcaccgatg accaccggac
     2581 cagccacctc gacggtcacc ctgaccgacg aggagcagga gaccgaggac ggcccggtcg
     2641 cccacatcgt ccggacgctg ccgggagagg acgcggcggc caaggtgctg gcggctcggg
     2701 tggaggggac tccgctcgaa gcgctgtgcg ggcacgtctg ggtgccgtcg cgggacccca
     2761 agcagctgcc ggtgtgccag aagtgcaagg acatctacgg gatgtaccgc gacttcaacg
     2821 acggcctgga cgataccccg cggatgtagg accgcgcgga tgtgagactc cgcggacgtc
     2881 gcggaacgcc gtgcagcccg cgggacggag cgatcggagg tcaccacaat gagggccgtc
     2941 atcaccggtg ccagcggatt cctgggagcg cacctggccc ggaccctgaa ggaccgcggg
     3001 gtcgaggtcc gggccgcgga ccgcagccgc ggccccgccc tcgacgacgt ggacgtcgag
     3061 ttcgtcgaga tcgacgtcct cgacccggcc agcctgcgag ccgcgttcga gggtcaggac
     3121 cgggtgttcc acctggcggc gatcatctcg atcgtcggcg acccgaccgg cgaggtgtgg
     3181 cgggtcaacg tccacggtcc ccgcaacgcc gcgaccgtcg cgctcgagtg cggggtcggc
     3241 cggttcgtgc actgctcgtc ggtgcactcc ttcgacctgg aacgctgcgg gccgtcgctc
     3301 gacgagtcgg gcctgccgac cacccatccc gacgccccgg cctacgaccg ctccaagcgc
     3361 gccggggagg aggcggtccg cctggtcgtg cagaagggac tcgacgcggt ggtcgtcaac
     3421 ccgaccggga tcatcggacc cgacgaccgg ggtccgtcgc ggatgggtga gacggtcctg
     3481 cagttcatga cccgcaagat cccggtgggg atcaccgggc agttcgactt cgtcgacgtg
     3541 cgcgacgtgg tcgagggcct gatcgccgcc ggcgaccgcg gccgcaccgg cgagaactac
     3601 ctcctgtcgg ggtcacggct cagcatccgc gagctcggga tgctggtcga gcgctactcg
     3661 ggggtgccgg tcccgccgct gtcgatcccg ctgcggctgg tgaccccgct ggcgcccctg
     3721 gtgatgcaga tcgagcagcg gaccaagaag ccgatcttca cccccgacgc cctgcacgcc
     3781 ctgaagtact cgccggtggt cagccactac cgggcggcga ccgaactcgg ctacaggagc
     3841 cggccgatcc acgagacggt ccgagacacc gtcgcctggt tcgagtcgaa ctgaccctca
     3901 gcgcacgcgt cgatcgctca ggaccccgga tccgatccgg agctcgtcag gaacgcggga
     3961 cgcagcccgc gccggtgacg tcgacgatgg tcccgatcgt ggcccggcgg tcggtcgcct
     4021 cggcgaaccc ggcgtcgtca caggtcccga cccagcgacg accgtcgtcc aggtccgcga
     4081 tcacgaacgc gcgcaccggt cggccgtcga ggtaggtggc ggtcgcggtc accaccgttc
     4141 ccggcccttg cgcctcgccg gtgacgtcgc ggacaccggt gcgcgcggcg gccggctcgg
     4201 ccacgtcctc gatcagcaca tcgggcgccg ggcggccacc ccagacggtg atgccgggct
     4261 tggtcaacag ccccgacacc gtggtgacca tcgccaggtg ctcggggtgc tcgcggaggt
     4321 gtcggacgac ctcgacagtg gcctggtagg tgaagttgtt gagcgggccg ccggcgaagg
     4381 ccatcccgcc ggtcaccgtc ggcgccaccg cggtccgttg gtcgcggtcc aggtcgagct
     4441 cgcgctgctg gagccgcacc gcgacgggga agcagctgta gatctcctgg atctcgatgt
     4501 cgcgcagcgg tcgaccgatg tgctcggcga cggcacgtcc gaccacaccc atcgcgggcc
     4561 agcggtggat ctcggatcga cggctgagcg acaccatgtg cgacgagtcg acgcacgcgt
     4621 gcgggaacac ccaccgctcg gccggaactc ccgcggcgcg cgccgcgtcg accgagcaca
     4681 ggatcagcgc cgcggcctgg tcgacgaccc actggctgac gtgccacttg ttgtacggcg
     4741 aggcgatggc ccggttgccg gggccgggat cacgcagctc ggcggcggtc cacggagctg
     4801 cgaacgcggc ggtcgggttg gtggtcgcca ccgcgttgta cgcggcccag agctcggcga
     4861 tgtcgtcgag ctgggagtcg aacggctggc cctcggcatg accgagcgcg ttctcgatgc
     4921 aggcgtactg ctcgacggcg gcccacatgc cggcggcgac ctcggcagcg gccatgatct
     4981 cgccggccgg cgaccaccgt tcgtcgggcg ttgcgccgtc ctgggcggtc tcgtcgatgg
     5041 gggtgccggc ccgggcagcc gcgcggctgc tcgccatcgc ctcgccgccg acgacgagag
     5101 caacgtcgag agcaccccgg cggatgcggt cgagggcgac cgcgaccggc tgggtctgcg
     5161 ggacgccgat gtcggcacgg accgttcggg ctctcctcgc ccccaccctg tcggcgacga
     5221 gccgggcgac gtcggggtag gaccacgagc cttcgggcac cgcgaccagt ccgcagtcgg
     5281 ggaggagccg agcggcgccg gcgtcgtcgg ccgcggcgag ggcggcctcg accatcagct
     5341 cgagcggttc gaccgcaccg gacccgtcgg cggggcggtg ggcggctccg acgccgacga
     5401 ggaccggggt gcggggatcg agggtggcgc tcacggtcgg tgacgctaac ggcgtgcagg
     5461 caccggagcg cgcccgacgc ggatcagggt gcgacgccgc acccgatccg cgtcgagccc
     5521 ccgccgccca ggctgtgggc caccacgcac accgtcgtgc cgggcgctgc gggcacgtcg
     5581 gacacgtgga acccgtgctc cgcgccgcga ccgaacgcgg cgtcgacgtc aggccggtcg
     5641 accgaggcgg tggcggtggc gaccgggttg ccggccacgt agaccgagac cttgaccggg
     5701 tcgtcgtcgt gggcgtcgag agcccagccg tcgacgctga cggttcccgc tccgccgtgg
     5761 accatgtcga gcgtgccggt cggcgagtcg gcgatgcgtt cggcgtcggt gcgcaccagg
     5821 gtccgagcgg cgcgcagcga cgagtaggcg ttgacgacgg tgccgtcggg ccgacgcagc
     5881 tcgaagtgca ggtgcgggct ggtcgactcg gcgttgccgg agtcaccgac gtagccgacc
     5941 acctcaccgg ccttcacctt ctggcccttg gcgatcccgt ccgcgaaggc ccgctcgtag
     6001 acgttggagc cgtcgtcggt gccgggcgcg tcgttgttga ggtggatgta gacgtagcgc
     6061 caaccgtcgg cgtcggtgat ggtcagcgag ttgcccgaca gcccgctgtt ggaccacgtc
     6121 agcgaggtga cggtgccgtc ggccgccgcg accagcggcg tgtggcgcgc cgccatcacg
     6181 tcctggccct ggtgggtgcg tccgccggag cgcggggccc cgaaggtgtc gctgtaggag
     6241 gtccggccgg cgacggggaa caccatctcg cgctcggccg gaccgaccgg cgccggagga
     6301 cggggcgggg ccggcacgca tgcggccagg aacgagacga ggccgacggc caggaccggg
     6361 agcaggacga acgggcgcgg gcgtggacgg tggccgaggg cggacatggc cccggtgatc
     6421 ggcaacccgg gcggaaacct tgaccgccac gtaggttgac ccgagcccgc gcagccgagg
     6481 gctctcacac cggaggagac gtcaatgacc gacgccgtgc tcgtcgacac gcccgccgag
     6541 ggggtccgcc gcatcacgct gaaccggccc gacaagcgca acgcgctcaa ccacgccgtg
     6601 cgtggagggg ttctcgacgc gctgcgcgag gcggatgagg accccgaggt ccgcgtgtcg
     6661 atcatccgcg gcgcggggag ctgcttcagc gccggctacg agctcggcga cggcaacgag
     6721 ggccacgacc tcccgtactt caccccggga ggcgagggct tctggccccg tcacgtgacc
     6781 gacgcgtgga tgagcatctg ggacctggcc aagccggtca tcgcccaggt ccacgggtgg
     6841 tgtctggccg gcggcagcga gctggccacc ggctgcgacc tggtctacgt gtccgacgac
     6901 gcccgcatcg ggtacccggc ggtgcgcttc ggggtgcccg acatgcagtt ccacgcctgg
     6961 ttcctggggc cgcgggccgc catggagatg atgctgaccg gcgactggat ctcgggcgag
     7021 gacgcggttc gcctcggctg ggccaaccgg gtgttcccgg cggaccgcct ggatgacgag
     7081 gtgctggcga tggccgagcg catcgcgctg gtgccgcccg acatcgccca gctgaacaag
     7141 cggaccgtgc accgggcgat ggacgtgatg ggtctgcgca ccgcgatccg ctcgggcacc
     7201 gagctgtgcg cgctgggagt gcaccagccc acgttccggg ccttcctcgg cgatgccggc
     7261 atcgacgggg ccgtcggcgg tgagcgcacc gatggcggga gcctgaccga ggcgttgcag
     7321 cggcgcgacg agccgttcgg cgactaccgc accggcgggg gtaccgaacc gtcctgaggc
     7381 cgcgaaggcc ctctccaccg ggtcgcccga aagggtcgga gtgggccccg tggggctcga
     7441 acccacgcgc gtcggattaa aagtccgctg ctctgccaac tgagctagag gcccgggcgt
     7501 tccgcggaat gctccgcggc tgtccaggtc cagatcatcc c
//
LOCUS       DAOQOD010000149         7465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3735_length_7465_cov_11.384615, whole genome shotgun sequence.
ACCESSION   DAOQOD010000149 DAOQOD010000000
VERSION     DAOQOD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_7465_cov_11.384615"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="nusB"
                     /locus_tag="PLZ72_12645"
     CDS             complement(<1..473)
                     /gene="nusB"
                     /locus_tag="PLZ72_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNA37191.1"
                     /translation="MPDEIADRVGPDARVVGSEVVLPPVVPQVAGGRREARERAVHLL
                     YESRMKTRSGPETVADQVAAPDDYAVDLVQGVEDHRDEIDGIIGRLARGWTIERMPAL
                     DLEVLRVACYELGHRPDVPRGVVLAEAVELATRYGTDDSSRFVNGLLAAAADELRP"
     gene            complement(542..1105)
                     /gene="efp"
                     /locus_tag="PLZ72_12650"
     CDS             complement(542..1105)
                     /gene="efp"
                     /locus_tag="PLZ72_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNA37192.1"
                     /translation="MPQISTSDFKNGMTVDLDDGLFSLVEFQHVKPGKGGAFVRTTLK
                     NVRNGSVVERTFRAGERMERAIIDKREMQLLYRDGDDYVFMDNSTYDQMNVPSATLGD
                     VSNFLVDSSTALLQMYGDEIVGVEVPASVELTVAETEPGVQGDRVSGAKKPATLETGL
                     VVQVPLFIEPAERIKVDTRTGEFISRA"
     gene            complement(1155..2297)
                     /locus_tag="PLZ72_12655"
     CDS             complement(1155..2297)
                     /locus_tag="PLZ72_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNA37193.1"
                     /translation="MTVTESPNLAPPPTDLPAMEVSARLGRLRDRLDSAGCDALVVTE
                     LTNIRYLTGFTGSAAMVLVTATDAVFVTDGRYRDRSADEMAAAGVVADIRIVTGAADE
                     AIAEAALDAGVGRLGLEADHVSWSAQQRWADQLLPDGDLVATTGVVEALRLVKDDGEV
                     ARIRSACAIADRALAQIRGRLLDGPTEAEFAAELDTAMRSEGAADVSFETIVASGPNG
                     AKPHHTPSDRRIAPGDLVVIDFGALVDGYHSDMTRTVAVGDVGDERTRMLDVVLAAQD
                     AGVHAVRAGVEAAEVDRACRSVIEDAGWGEAFLHSTGHGVGLDIHEEPRVGARSAATL
                     DAGQVVTVEPGVYLTGLGGVRIEDTVLVTDDGCDRLTLAPKDPILA"
     gene            complement(2413..2874)
                     /locus_tag="PLZ72_12660"
     CDS             complement(2413..2874)
                     /locus_tag="PLZ72_12660"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNA37194.1"
                     /translation="MTDPTHRSILLLSGPNLNLLGERQPEVYGTATLDAHVATAAERA
                     QQAGVEVEHVQSNAEAVLVEAVHRARGAHDAIVVNAGAVTHYGWGLHDALAAFDGPVV
                     ELHLSNPSAREPWRHTSVVSPVADVVIQGAGGLGYRLAVDAALGLLGLDLP"
     gene            complement(2878..3921)
                     /locus_tag="PLZ72_12665"
     CDS             complement(2878..3921)
                     /locus_tag="PLZ72_12665"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HNA37195.1"
                     /translation="MNELIEVTVPLGGGRDYPVLVGDGALGSLPDLVPDHARRVAVVT
                     QSGIGIDVEPGVEHRVFTIGDGEPAKTTATVADLCSEFARWGLTRSDCVVGVGGGLVT
                     DVAGFAAAVYHRGIPVVHCATSLLGQIDAAIGGKTGVNLPEGKNLVGAYWQPRGVICD
                     TATLASLPPREWRCGLGELAKYHWLGGGRLDELDLPERVAACVAIKADVVASDEREGG
                     RRALLNYGHTLAHSLETVGHHDLRHGEAVAIGLVFAAELARDMGRIDDAAVAEHRRVV
                     SGYDLPVEVPAGLDTDELVEVMGRDKKVLGEGLTFVLDGPTGVEVVPGVDASAVRATL
                     ARFRGETGSEGRS"
     assembly_gap    3973..4072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4073..4553)
                     /locus_tag="PLZ72_12670"
     CDS             complement(<4073..4553)
                     /locus_tag="PLZ72_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNA37196.1"
                     /translation="MPPTTDPTGPLAHLVLVGLSGTGKSTVAPLLAERRGVVAVDLDR
                     LLEQRFGRPVAQVFLEDGEPEFRRAEAELLAEVLVGPPAVIATGGGAVLDPESRRRLA
                     DAAFVVWLSAPIDLLVRRLSDVAERRPLLADDPATALRAMAAERDPLYREVADLVVDV
                     "
     gene            complement(4574..5509)
                     /locus_tag="PLZ72_12675"
     CDS             complement(4574..5509)
                     /locus_tag="PLZ72_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37197.1"
                     /translation="MSGSTQGDGADPSGVKPDAVGRGWIDRGGPTPEPALFFLSGTSL
                     GIGNLVEGSLPAWIELREATNLDALGDPENGLQAVEMAISGGQVVRAGWPLNFCDHVV
                     SALQATVAATEPPTAAVTAPVPPAPIAPTPVAPTSVAPTPAPTAPAPTPTPAASADAP
                     TPSIAPGVPAGAAPGPAVPGAPPVDPSIPTESTAAAAAALVLEDVTYLGGYPGQSKKR
                     KKCTATLTRDAVEVAGPNGLTFRVPWEVIRTIEAQNADEARFRMNTKVHRDATALVME
                     CDQGVTVLLEARDCPTIPLRGAIAQLVGDMPVVVV"
     gene            complement(5627..6985)
                     /locus_tag="PLZ72_12680"
     CDS             complement(5627..6985)
                     /locus_tag="PLZ72_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNA37198.1"
                     /translation="MALQGTIDAFPVVDVLQLLAGSRKTGRLIVEGDRSTAQLFVVDG
                     SATGGGVQGVDDSALVDVVAELLRYREGSFLFEPGSEAPQPQDPEDLPALVDAAGEAM
                     AAWAAIEAVVPSMSHRLSLCSEISDEGVHLSAADWPVVVAAGAGSRVGELVSASGASE
                     IETCSSIAALVERGIVAVQAPTAELVLAEAPVAGPSVDPVTAGDVDPSAGDRASSDGD
                     VDTHSGDGDVDGSTEDVAEYEVVLLDEDDDGAFPEHFPIDDLLGGADEEDPWVQLESA
                     GREDRLAAAQTFGDEPSGPGADEAFSAGGLGSFDNSAGFDSPAAFDSPAAFGGSATFD
                     SPAAFDSPAAFDSPAAFGGFEGASGFQDSGTLTDPVAFGSDPMTASPGGVGATFVGDG
                     AFTAEPSRTPLAVQDPVRTDAAADNAADEVLRQMSKLSPKAAEAIAAALGSASDQDDP
                     AT"
     gene            complement(7110..>7465)
                     /locus_tag="PLZ72_12685"
     CDS             complement(7110..>7465)
                     /locus_tag="PLZ72_12685"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37199.1"
                     /translation="RGEHAAALAGPDGRFVPLDEDRAVRTAIADVDLLVDATPTGMAG
                     DSSISVPEACLRADLAVVDLVYHPTETPLLTAARREGSKTANGLSMLVHQAAVAFEHW
                     TGTAAPLDVMVAAIA"
BASE COUNT         1040 a         2623 c         2677 g         1025 t
ORIGIN      
        1 ggtcggagct cgtcggccgc ggccgcgagc agtccgttga cgaacctcga ggagtcgtcg
       61 gtgccgtagc gggtggccag ctcgaccgcc tcggccagca cgaccccccg ggggacgtcg
      121 ggacgatggc cgagctcgta gcaggcgacg cggagcacct ccaggtccag cgccggcatg
      181 cgctcgatgg tccaaccccg cgccagtcgg ccgatgatcc cgtcgatctc gtcgcggtgg
      241 tcctcgaccc cctgcaccag gtcgaccgcg tagtcgtcgg gggcggcgac ctgatcggcc
      301 accgtctccg gaccgctgcg ggtcttcatc cggctctcgt agagcaggtg caccgcgcgc
      361 tcacgcgcct cgcgccgacc gccggcgacc tgtgggacca ccggggggag cacgacctcg
      421 gagccgacga cccgggcgtc gggaccgacg cggtcggcga tctcgtccgg caacggctcg
      481 ggcgccggct ccggagttcc gctcggagtc ccgctcggag tcggagtcgg atccggtccc
      541 atcaggcgcg ggagatgaac tcgccggtgc gggtgtcgac cttgatacgc tcggccggct
      601 cgatgaacaa cgggacctgc acgaccagcc ccgtctcgag ggtggcaggc ttcttggcgc
      661 ccgagacccg gtcgccctgc actccgggtt cggtctcggc gacggtgagc tccaccgacg
      721 cggggacctc gacaccgacg atctcgtcgc cgtacatctg cagcagcgcg gtcgaggagt
      781 cgacgaggaa gttcgacacg tcgccgaggg tggccgacgg gacgttcatc tggtcgtagg
      841 tcgagttgtc catgaagacg tagtcgtcgc cgtcgcggta cagcagctgc atctcgcgct
      901 tgtcgatgat cgcccgctcc atgcgctcgc cggcacggaa ggtgcgctcg acgaccgagc
      961 cgttgcggac gttcttgagc gtggtgcgca cgaacgcgcc gcccttgccc ggcttgacgt
     1021 gctggaactc gaccagggag aagagcccgt cgtcgaggtc gacggtcatc ccgttcttga
     1081 agtcggaggt ggagatctgg ggcatggcta tcggcctgtc tgttcggccc gacacggctc
     1141 gggggcggcc gcgttcaggc gaggatcgga tccttggggg ccagggtgag gcggtcgcaa
     1201 ccgtcgtcgg tgacgaggac ggtgtcctcg atgcggacgc cgcccaaccc ggtgaggtac
     1261 accccgggct cgacggtcac cacctggccg gcgtccaagg tagcagcgga gcgggctccg
     1321 acccggggct cctcgtggat gtcgagtccg acgccgtgtc cggtcgagtg caggaacgcc
     1381 tctccccacc ctgcgtcctc gatcaccgag cggcaggccc ggtcgacctc ggcggcctcg
     1441 acgccggccc gaacggcgtg cacgccggcg tcctgggcgg cgaggacgac gtcgagcatc
     1501 cgggtccgct cgtcacccac gtcgccgacg gcgacggtcc gggtcatgtc ggagtgatag
     1561 ccgtcgacca gggcgccgaa gtcgatcacc acgaggtctc cgggtgcgat gcgacgatcc
     1621 gacggcgtgt ggtgtggctt ggctccgttc gggcccgagg ccacgatcgt ctcgaagctc
     1681 acgtccgctg ccccctcgga gcgcatggcg gtgtcgagct cggcggcgaa ctcggcctcg
     1741 gtcggcccgt cgaggaggcg ccctcggatc tgggccaggg ctcggtccgc tatggcgcac
     1801 gctgagcgga tgcgggcgac ctctccgtcg tccttgacca gccggagcgc ttcgacgacg
     1861 cccgtggtgg ccaccaggtc accgtcgggg aggagttggt cggcccagcg ctgctgcgcc
     1921 gaccagctga cgtggtcggc ctccagaccc agacggccga cgccggcgtc gagggccgcc
     1981 tcggcgatcg cctcgtccgc cgcaccggtg acgatgcgga tgtcggcgac gacaccggct
     2041 gcggccatct cgtcggcgct gcggtcgcgg tagcggccgt cggtgacgaa gacggcgtcg
     2101 gtcgcggtca ccaggaccat cgcggccgat ccggtgaagc cggtcaggta gcggatgttg
     2161 gtcagctcgg tgacgaccag tgcgtcgcaa ccggcgctgt cgagtcggtc ccggagccgc
     2221 ccgaggcgtg cggagacctc catggccggc aggtcggtgg gcggtggcgc cagattcggt
     2281 gactcggtga ccgtcatggg ctccacccta cggcccgcgc catcacaccc tcccgcccgc
     2341 gacccgttct gttcgttgga tcacaactcc acggatgtga tccaacgaac agaacgggtc
     2401 agggggtccg ggtcagggga gatcgaggcc caggagtccg agggcggcgt cgaccgccag
     2461 ccggtacccc agtccacccg caccctggat gaccacatcg gccaccggcg agaccaccga
     2521 cgtgtgtcgc cacggctcgc gggcggacgg gttggacagg tgcagctcca ccaccggacc
     2581 gtcgaacgcg gccagggcgt cgtgcaaccc ccagccgtag tgtgtgaccg ctccggcgtt
     2641 gacgacgatc gcgtcgtggg ccccgcgcgc ccggtgcacc gcttcgacga ggacggcctc
     2701 ggcgttggac tgcacgtgct cgacctcgac gccggcctgc tgggcgcgct cggccgcggt
     2761 ggcgacgtgc gcgtcgaggg tggccgtccc gtagacctcg ggttggcgct ctccgagcag
     2821 gttcaggttc ggccccgaca acagcaggat cgatcggtgg gtcgggtcgg tcacgtctca
     2881 gcttctcccc tcggaaccgg tctccccacg gaagcgggcc agcgtcgccc gcaccgccga
     2941 ggcatcgacg ccggggacga cctccacccc cgtcggcccg tcgagcacga aggtgagtcc
     3001 ctcccccagc accttcttgt cgcggcccat gacctcgacc agctcgtcgg tgtcgaggcc
     3061 ggcgggcacc tcgaccggca ggtcgtagcc cgagacgacc cgtcggtgct cggcgaccgc
     3121 ggcgtcgtcg atgcgaccca tgtcgcgggc cagctcggcg gcgaacacca gtccgatggc
     3181 gacggcctcg ccgtggcgga ggtcgtggtg accgacggtc tcgagcgagt gggcgagcgt
     3241 gtggccgtag ttcaacagcg cccgtcggcc cccttcccgc tcgtcggagg cgaccacgtc
     3301 ggccttgatg gcgacgcacg ccgcgacccg ttcgggcagg tcgagctcgt ccaggcgccc
     3361 gccgccgagc cagtggtact tggcgagttc gccgagaccg cagcgccact cccggggcgg
     3421 caacgaggcc agcgtcgcgg tgtcgcagat cacacccctc ggctgccagt aggcgccgac
     3481 gaggttcttg ccctcgggga ggttgacccc cgtcttgccg ccgatggcgg cgtcgatctg
     3541 tccgagcaga gaggtcgcgc agtgcaccac ggggatgcct cggtggtaca ccgcggcggc
     3601 gaacccggcc acgtcggtca cgagcccgcc gcccacaccg accacgcagt ccgagcgcgt
     3661 cagcccccaa cgggcgaact ccgagcacag gtcggcgacg gtggccgtgg tcttggccgg
     3721 ctctccgtcg ccgatggtga acacacggtg ctcgaccccg ggttcgacgt cgatgccgat
     3781 gcccgactgg gtgaccaccg cgacccgtcg tgcgtggtcg ggaaccagat cgggcagcga
     3841 cccgagggcc ccgtcaccga ccagcaccgg gtagtcgcgg ccgccgccga gcggaaccgt
     3901 cacctcgatc agctcgttca tcgggcaccc cgcgtccggt cggtcgtcgg cgagggctcg
     3961 ccggtggtgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgacgtcg
     4081 acgacgaggt cggcgacctc gcggtagaga gggtcccgct ccgcggccat ggcccgcaag
     4141 gcggttgcgg ggtcgtcggc cagcaggggg cgccgctcgg cgacgtccga caggcgccgg
     4201 accagcaggt cgatcggggc ggacagccag accacgaagg cggcgtcggc cagccggcga
     4261 cggctctccg ggtcgaggac cgctccccca ccggtggcga tcaccgcggg cgggccgacg
     4321 aggacctcgg ccaggagctc ggcctcggcc cgccggaact ccggctcgcc gtcctcgagg
     4381 aacacctggg cgaccggacg gccgaagcgc tgctcgagca gccggtcgag gtcgaccgcg
     4441 acaacgcccc gacgttcggc gagcaggggg gcgacggtcg acttgccggt cccggagaga
     4501 ccgaccagca ccagatgggc gaggggcccg gtcgggtcgg tcgtgggcgg caacggcgtg
     4561 gggtcggtcg tgctcagacg accacgaccg gcatgtcgcc gaccagctgg gcgatggcac
     4621 cccgcaacgg gatggtgggg cagtctcggg cctccaggag aaccgtcaca ccctggtcgc
     4681 actccatgac caacgccgtg gcgtcgcggt gcaccttggt gttcatgcgg aagcgggcct
     4741 cgtcggcgtt ctgggcctcg atcgtgcgga tgacctccca cggcacccgg aaggtcaacc
     4801 cgttgggccc ggcgacctcg acggcgtcac gggtgagcgt ggcggtgcac ttcttgcgct
     4861 tcttgctctg gcccgggtag ccgcccaggt acgtgacgtc ctccagcacc agagcggccg
     4921 cggccgcagc cgtgctctcg gtggggatcg acgggtcgac gggaggtgct ccgggaaccg
     4981 cgggcccggg cgccgccccg gccgggacgc ccggagcgat cgacggggtc ggggcgtctg
     5041 cagacgctgc gggagtggga gtgggagcgg gagcggtcgg tgccggcgtg ggagcaaccg
     5101 aggtcggagc aaccggggtt ggcgcgatcg gggctggcgg aaccggtgcg gtgaccgcgg
     5161 cggtgggggg ctcggtcgcc gccaccgtcg cctgcagcgc gctgacgacg tggtcgcaga
     5221 agttcagggg ccagccggca cgcaccacct gaccaccgga gatcgccatc tcgacggcct
     5281 gcagcccgtt ctccggatcg cccagcgcgt cgaggttggt cgcctctcgc agctcgatcc
     5341 aggccgggag cgagccctcg accaggttgc cgatgcccaa gctggtgccc gacaggaaga
     5401 acagcgcggg ctccggggtc ggtcccccgc ggtcgatcca cccgcgtccg accgcgtccg
     5461 gcttcacacc cgacgggtcc gccccgtcgc cctgagttga tccgctcacg cctgaggacc
     5521 tccacacgtt cggcggctcc taccgtaccg gtcaaccgag cccgcgccac gcgacccccc
     5581 gtgtcggttc gagcggtccg cgtggggtga tcggcgaccc ggtcgctcag gtcgccgggt
     5641 cgtcctggtc cgaggcggag cccagggcgg ccgcgatggc ctcggcggcc ttcgggctga
     5701 gcttggacat ctgccgcagc acctcgtccg ccgcgttgtc ggccgcagcg tcggtgcgga
     5761 ccgggtcctg gaccgccaga ggggtccggg acggctctgc ggtgaacgct ccgtcgccga
     5821 cgaaggtggc cccgacgcca ccgggagacg cggtcatcgg atccgatccg aaggcgacgg
     5881 gatcggtgag ggtgccggag tcctggaacc ccgatgcccc ctcgaagccg ccgaacgcgg
     5941 cggggctgtc gaacgcggcg ggactgtcga acgcggccgg actgtcgaac gtcgctgagc
     6001 caccgaacgc ggctggactg tcgaacgccg ccggactgtc gaacccggcc gagttgtcga
     6061 acgaaccgag gccgcccgca gagaacgcct cgtccgcacc gggaccgctc ggctcgtcgc
     6121 cgaaggtctg ggccgcggcc agtcgatcct cccgaccggc ggactcgagc tggacccacg
     6181 ggtcctcctc gtcggccccg ccgagcaggt cgtcgatcgg gaagtgctcc gggaaggccc
     6241 cgtcgtcgtc ctcgtcgagg aggacgacct cgtactcggc gacgtcctcg gtcgacccgt
     6301 cgacgtcgcc gtcacccgag tgggtgtcga catctccgtc gctcgacgcc cgatcgccag
     6361 ccgacggatc gacgtcgccc gccgtgaccg gatcgaccga cgggccagcg accggagcct
     6421 cggccaggac gagctcggcg gtcggagcct gcacggccac gatgccacgc tcgaccaggg
     6481 ctgcgatcga cgaacaggtc tcgatctcgg aggcccccga agcggacacg agctcaccga
     6541 cacgggaacc ggcacccgcc gcgaccacca ctggccagtc cgcggcgctg aggtggacgc
     6601 cctcgtcgct gatctcgctg cagagggaga gccgatgtga catggacggg accacggcct
     6661 cgatcgcggc ccaggccgcc atcgcctccc cggcggcgtc gaccagagcg ggcaggtcct
     6721 cgggatcctg gggctgcggg gcctccgagc ccggctcgaa caggaacgaa ccctcccggt
     6781 agcgcaggag ctcggcgacg acgtcgacga gagccgagtc gtcgacgccc tgcacgcctc
     6841 caccggtcgc cgatccgtcg acgacgaaga gctgggcggt gctgcggtca ccctcgacga
     6901 tgagtcgacc ggtcttgcgc gaccccgcca gcagctgcag cacgtccacc acggggaagg
     6961 cgtcgatggt tccctggagt gccacgtttg atcctcctcg aagccgcgac cggcctcctc
     7021 gaactttcct gttgggtctc atcggcagga accggcggcg ggttcagcaa tttcctgggc
     7081 cgatcgaccc agcgaggaga cagccgttct caggcgatcg cggcgaccat cacgtcgagg
     7141 ggggcggcgg ttccggtcca gtgctcgaac gcgaccgcgg cctggtgcac cagcatcgac
     7201 agcccgttgg cggttttcga cccctcacgc ctggccgccg tgagcaacgg ggtctccgtc
     7261 gggtggtaga cgagatcgac cacggccaga tcggcgcgga ggcaggcctc gggaaccgag
     7321 atcgaggagt cgcccgccat ccccgtcggt gtggcgtcca ccagcagatc gacgtccgcg
     7381 atcgccgtcc ggaccgcccg gtcctcgtcg agggggacga agcgcccgtc gggtccggcc
     7441 agggcggccg cgtgctcgcc ccggc
//
LOCUS       DAOQOD010000150         7457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3744_length_7457_cov_12.806944, whole genome shotgun sequence.
ACCESSION   DAOQOD010000150 DAOQOD010000000
VERSION     DAOQOD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3744_length_7457_cov_12.806944"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLZ72_12690"
     CDS             complement(<1..1002)
                     /locus_tag="PLZ72_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA37200.1"
                     /translation="MELNLADLFESVAATVPDRPAIVAGARRLTYRELDERADRLAAV
                     LAAAGVGRDDFVGIQLPNGTEYLEAMIAAFKLRAVPVNVNYRYVDAELRHLYSDAGLV
                     ALVHHTDFADAVAGAVDAMAERRVVLAVGPGGDYEDALAAATASSDYAPRSADDLYCI
                     YTGGTTGLPKGVLWRHEDIFFTAMGGGDPFQLGDHITRAEQLAGRIPDFPLVALPAPP
                     FMHAAAHWLAFAVLFGGGKVVTLPEGRFDPAATWRLVDDEGVNILVVVGDAMARPLAD
                     ALAADPGAYDLSTLMAMGSGGALLSPSTKARLRELRPGLIVRDAFGSSETGQIGGEPP
                     "
     gene            complement(1007..2167)
                     /locus_tag="PLZ72_12695"
     CDS             complement(1007..2167)
                     /locus_tag="PLZ72_12695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA37201.1"
                     /translation="MEGTSVTVDGFAEPGFSAVADAFAANFAERGDVGAAVCVYHRGR
                     TVVDLWGGWADREAGRPWSRNTLQLVFSTTKGMTATCVHLLAQRGELDLDAPVAAYWP
                     EFAAAGKADIPIRWVLSHRAGLAAVTGDLTLDDVLGWDGVVDAIAAQAPVWEPGTVHG
                     YHARSFGWILGEVVRRVSGRSLGRFFAEEVAAPLDLDFFIGLPASELPRVSHLYPPVI
                     DPATQEVMDAFMGPDTLLGQVLSGPSGLFGYDDMWNRPEVLAAEMPSSNGVGTARAVA
                     RMYAALVGEVDGVRLLDADAVVAAIEVQSSGPDHVLGVPMSFGSGFMSPSPSGPPGSF
                     GHDGAGGSLGLADPGGGWSLGYVMNQMQLGVAGDPRSAALVDAVVASLEKGA"
     gene            2333..3553
                     /locus_tag="PLZ72_12700"
     CDS             2333..3553
                     /locus_tag="PLZ72_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNA37202.1"
                     /translation="MADPTAPYPDRSAVEEAFAAHVNRGKVDAYRALGLELVMGERDG
                     IRFRDAYDGTWYVNCHCNGGVFNLGHRHPAVVAAVRDALETLDVGNHHLVSGWRARLA
                     ERLSATTGGALPGVVFGVGGGEAIDLAIKVARAHTGRAGVVSVTGGYHGHTGLALAAG
                     DPAYRDPFGPNPPGFTQVVFDDLDAMAAAVDERTAVVLVEPIPATLGMPIPSPGYLAG
                     LRTLCDERGALLAFDEVQTGLGRTGTVWYHVQEGVTPDLLVTGKGLSGGVYPITATLM
                     TAELHRFFDDHPFVHVSTFGGAELGCVAGLATLDVVEAPGFLDRVVELGERFESELAD
                     MPFQLRRRGMFMGLAFDDPAGGMTATQALVSAGVFAIFANNDPSVLQFLPPLTIADDE
                     VDDLIATLRRVLGS"
     gene            3578..4531
                     /locus_tag="PLZ72_12705"
     CDS             3578..4531
                     /locus_tag="PLZ72_12705"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HNA37203.1"
                     /translation="MADGSTPEDDVSDGREAGVGFRPGDVPIIDLDAVEAAVQQALER
                     GDGSGLTLLGYGEISLVVGWPTGAPVVAAKRLPPFPSADAAADYGRRFVAYLDRLAER
                     GVVPVASAFRVVVGTGSTRVAYVTQAVLDPDDLGPAILRRSAPDPDHPLLRGIVDAVG
                     AVCDPRTGLDAQVSNWALVDGQLRYLDVTTPMLFDPSGRAELDLGLFLAAYPWALRGL
                     IGRFVAPDVIGAYRDPRRVGVDLAGNLLKERLAEWIPSVIEAVNRTVEAEITEDEVRR
                     YYRSDARLWELMLRLRRADRWWQRRIRRRTYPFLLPGHIER"
     gene            4632..5660
                     /locus_tag="PLZ72_12710"
     CDS             4632..5660
                     /locus_tag="PLZ72_12710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNA37204.1"
                     /translation="MHDDVTADGSSADGTDSHDGSATGGDRAPAGRKPRRSTAEGAEI
                     RPGSLLWTTAGDPRAMIPGLATGIMQLMLPGLGAGVTDYSDFFNDPFDRIDRSIPLIW
                     GSIFAPDDEEGRVRGRQIRDFHVDIKGTDGHGKRYHALDPDVYWWAHATFTWEYFRAR
                     ELFFPCPLTRRQKDRLYAETVTWYRRYGVSDRPVPATYREFRHRFDEICRHELELTPA
                     VQWVLDQSTNPAATADPVRLPGMWSVLDGLVTNLGTETLRLLVYGPMPDVVRRRFDLP
                     WSHSDRVAFTALCATIRAMDPAIRRGALDAIYPEGTPHPDRRDPTRLVVAGPNRRQRA
                     LAAARSAA"
     gene            5810..6259
                     /locus_tag="PLZ72_12715"
     CDS             5810..6259
                     /locus_tag="PLZ72_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNA37205.1"
                     /translation="MGAVEPTSSVTTAGDLSTRTQIHDLVVDFYREIVFDDLLGPVFV
                     DVAEVDWAVHIPILVDYWCRIVLGLPGSNARVTAVHRHVHALEPLGVEHCDRWYELWT
                     ACLDARWSGPLAERARSHAAALMSGMARHVFDVEWQAPSAAPAGALS"
     gene            6256..7158
                     /locus_tag="PLZ72_12720"
     CDS             6256..7158
                     /locus_tag="PLZ72_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNA37206.1"
                     /translation="MTATDPLDDLQIDLTDHAARSREERPATTAPASGAAPVPRQRSM
                     LRSVALPSEHGGWGLTGEPIVLGLLLAPSLAGLLVGLAGMIAFVARTPVSLVLVDGFR
                     HRDLERTRLARRVAAIEISALVVLVAGATALAGPGFWWPVAIAAPLVSVQLWFDRRSR
                     SRRLLPELAGAVGITSLVAAITLAGGGDTAVAVGAWAILAARALTSIPFVKSLVMKLH
                     GKATAQWTLITWDVVAVAIVVTAAWLEPTLTAGAGAVVAVVALQRLTTLRPPPRPTVT
                     GIRQTFMGLAVVVATWLGVLMGGA"
     gene            7155..>7457
                     /locus_tag="PLZ72_12725"
     CDS             7155..>7457
                     /locus_tag="PLZ72_12725"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNA37207.1"
                     /translation="MKNDADRSGSTEGIDAGSGSGPARVLVDATESGVRVRVHPGLCE
                     GWGNCHRWSPEVYPLDDEGYIAIHMLEVPPELARLAWRGADACPAQAISVIGERPPP"
BASE COUNT         1015 a         2737 c         2649 g         1056 t
ORIGIN      
        1 gggcggctca ccgccgatct ggccggtctc cgacgaaccg aacgcgtcgc gcacgatgag
       61 ccccggacgc agctcgcgga gccgggcctt ggtcgacggc gacagcagcg cccctccgga
      121 gcccatcgcc atcagcgtcg acaggtcgta cgcgccgggg tcggcggcca gcgcgtcggc
      181 cagcggtcgg gccatggcgt cgccgacgac gacgaggatg ttcacccctt cgtcatcgac
      241 cagccgccac gtcgcggcgg ggtcgaagcg accttcgggc aacgtcacca ccttgccgcc
      301 gccgaacagg acggcgaagg ccagccagtg cgcggcggcg tgcatgaacg gcggcgccgg
      361 cagagcgacg agcgggaagt cggggatgcg cccggccagc tgctcggcgc gggtgatgtg
      421 gtcgccgagc tggaacgggt ccccgccgcc catggcggtg aagaagatgt cctcgtggcg
      481 ccacagcacg cccttgggca gcccggtcgt gccgccggta tagatgcagt acaggtcgtc
      541 cgctgagcgt ggcgcgtagt ccgacgacgc cgtcgccgcg gccagagcgt cctcgtagtc
      601 gccgccgggc ccgaccgcca gcaccacgcg ccgctcggcc atggcgtcga ccgccccggc
      661 gacggcgtcg gcgaagtcgg tgtggtgcac cagcgcgacc aggccggcgt cggagtacaa
      721 gtggcgcaac tcggcgtcga cgtagcggta gttgacgttg accggtaccg cacgcagctt
      781 gaacgcggcg atcatcgctt cgagatactc ggtgccgttg ggcagctgga tcccgacgaa
      841 gtcgtcacgt cccaccccag cggcggcgag cacggcggcc agccggtcgg cccgctcgtc
      901 gagctcgcgg taggtgagcc gacgggcccc ggcgacgatc gcgggacggt cgggcaccgt
      961 ggcggccacg gactcgaaca ggtcggccag gttcagctcc acgccgtcag gctcccttct
     1021 ccagcgacgc gaccaccgcg tcgaccagcg ccgcgctgcg ggggtcgccg gcgaccccca
     1081 gctgcatctg gttcatgacg tatccgagcg accacccacc accggggtcg gcaaggccga
     1141 gggacccccc ggcgccgtcg tggccgaacg agccgggcgg accggacggc gacggcgaca
     1201 tgaaccccga gccgaacgac atcggcactc cgagcacgtg gtcggggccg gaggactgca
     1261 cctcgatcgc ggccacgacc gcatcggcgt cgagcagacg gaccccgtcg acctcaccga
     1321 cgagcgcggc gtacatccgc gccacggccc gtgcggtgcc gaccccgttc gaggatggca
     1381 tctcggcggc cagcacctcg ggtcggttcc acatgtcgtc gtagccgaac aggcccgacg
     1441 gacccgacag cacctgaccg agcagcgtgt cgggccccat gaacgcgtcc atcacctctt
     1501 gggtcgccgg gtcgatgacc ggcgggtaca ggtgcgagac gcggggcagc tccgaggcgg
     1561 gcagaccgat gaagaagtcc aggtcgagcg gcgcggccac ctcctcggcg aagaaccggc
     1621 cgaggctccg gccgctcacc cgtcgcacga cctcgccgag gatccagccg aaggagcggg
     1681 cgtggtagcc gtggacggtg ccgggctccc acaccggggc ctgcgcggcg atggcgtcga
     1741 ccacgccgtc ccagccgagc acgtcgtcga gcgtgaggtc gccggtcacc gcggccaggc
     1801 ccgcccggtg cgacagcacc caccggatcg ggatgtcggc cttgcccgcc gccgcgaact
     1861 ccggccagta ggcggccacc ggagcgtcca ggtcgagctc gccccgctgt gcgagcaggt
     1921 gcacgcaggt ggcggtcatc cccttggtgg tcgagaacac cagctgcaac gtgttccggg
     1981 accagggtcg gcccgcctcg cggtcggccc agccgcccca caggtccacg accgttcgac
     2041 cccggtggta gacgcacacc gcagcgccga cgtcgccgcg ctcggcgaag ttggccgcga
     2101 aggcgtcggc cacggcggag aacccgggct ccgcgaaccc gtcgacggtg acggaggtgc
     2161 cctccaccgc ggtcacacta atcacggcgt cgcggacccc gacgtcgatc ggacggaggt
     2221 caccacgacg agcggacccg accgatcggg tgggatcgct cgccgtggcc ggtccggtcg
     2281 tggttcgatc gtggagcacg tccgctcgat cccgatcgac cgaggagggc cgatggccga
     2341 tcccactgcg ccgtaccccg accggtccgc ggtcgaggag gcgttcgccg cccacgtcaa
     2401 ccgcggcaag gtcgacgcct atcgggcgct cggactcgag ctggtgatgg gcgagcgtga
     2461 cgggatccgc ttccgtgacg cctacgacgg cacctggtac gtcaactgcc actgcaacgg
     2521 cggcgtgttc aacctgggac accgccaccc ggcggtcgtc gccgcggtgc gcgacgccct
     2581 cgagaccctc gacgtcggca accaccacct ggtgtcgggg tggcgggccc gtctggccga
     2641 gcggctgtcg gccaccaccg gcggcgccct tcccggcgtg gtgttcggcg tcggcggtgg
     2701 cgaggcgatc gacctggcga tcaaggtcgc ccgcgcccac accggccgcg caggggtcgt
     2761 gtcggtcacc ggcggctacc acggccacac cggcctggcc ctcgccgcgg gtgaccccgc
     2821 ctaccgcgac ccgttcggac cgaaccctcc cggattcacc caggtcgtct tcgacgacct
     2881 cgacgccatg gcggccgcgg tcgacgagcg cacggcggtc gtgctggtcg agccgatccc
     2941 cgccacgctc ggcatgccga tcccgagccc cggctacctg gccgggctgc ggaccctgtg
     3001 cgacgagcga ggtgcgttgt tggccttcga cgaggtccag accggcctcg ggcgaaccgg
     3061 caccgtctgg taccacgtcc aggagggcgt cacccccgac ctgctcgtca ccggcaaggg
     3121 gctgtcgggc ggggtgtacc cgatcaccgc cacgctcatg accgccgagc tccaccggtt
     3181 cttcgacgac cacccgttcg tgcacgtgtc gacgttcggg ggcgccgagc tcggctgcgt
     3241 cgccggcctg gccaccctcg acgtcgtcga agcacccggc ttcctcgacc gggtcgtcga
     3301 gctcggcgag cggttcgaga gcgagctggc ggacatgccg ttccagctgc ggcgccgggg
     3361 gatgttcatg ggcctcgcct tcgacgaccc ggccggaggc atgaccgcca cccaggcgct
     3421 ggtgtcggcc ggggtgttcg cgatcttcgc caacaacgac ccgtcggtcc tgcagttcct
     3481 gccgccgctg accatcgccg acgacgaggt cgacgacctg atcgccacgc tgcgacgggt
     3541 cctcggttcg tgatgtccgc gacgggaggg ggaccggatg gctgatgggt cgacgccgga
     3601 ggacgacgtc tccgatgggc gggaggcggg cgtcggcttt cgccccggcg acgtgccgat
     3661 catcgacctc gacgcggtcg aggccgcggt gcaacaggct ctcgagcgag gggacgggtc
     3721 cgggctcacc ctgctcggct acggcgagat ctcgctggtg gtcggctggc cgaccggtgc
     3781 ccccgtcgtg gcggccaagc ggctgccgcc gttcccgtca gccgatgcgg ccgccgacta
     3841 cggccgtcgg ttcgtcgcct acctcgaccg actggccgag cgcggggtcg tcccggtcgc
     3901 gagcgccttc cgggtcgtgg tcgggaccgg ctccacccgc gtggcctacg tgacccaggc
     3961 cgtcctcgat cccgatgacc tgggtccggc gatcctgcgg cggagcgctc ccgatcccga
     4021 ccacccgttg ctgcgcggca tcgtcgacgc ggtcggcgcc gtctgcgacc cgcggaccgg
     4081 gctggacgcc caggtgtcca actgggcgct cgtcgacggg caactgcgtt acctcgacgt
     4141 caccacgccg atgctcttcg atccctcggg acgagccgag ctcgacctcg ggttgttcct
     4201 ggccgcctac ccgtgggcgc tgcgcggcct gatcgggcgg ttcgtggccc ccgacgtgat
     4261 cggcgcgtac cgcgacccgc gccgggtggg cgtcgacttg gccggcaatc tcctcaagga
     4321 gcgcctcgcg gagtggatcc cgtcggtgat cgaggcggtc aaccggaccg tcgaggccga
     4381 gatcaccgag gacgaggtac gccgctacta ccgctcagat gcacgcctgt gggagctcat
     4441 gctgcggctg cgccgagccg accggtggtg gcaacgccgg atccgccggc ggacctaccc
     4501 gttcctgctg cccggacaca tcgaacgctg acgtggtcga tggctgatga tcggtgccgt
     4561 gccatgggag cccggagtgg cggacgggcc gtcgggcgtg gctaccttgt tgccgaccac
     4621 ggggggatga catgcacgac gacgtgaccg cggacggctc gagcgccgac ggtaccgaca
     4681 gccacgacgg atccgcgacc gggggcgacc gggcgccggc gggccgcaag ccccgacgct
     4741 cgaccgccga gggggccgag atccggcccg ggtcgctgct gtggacgaca gcgggcgacc
     4801 cccgggcgat gatccccggt ctcgcgaccg ggatcatgca gctgatgctg ccgggactcg
     4861 gagccggcgt caccgactac tcggacttct tcaacgaccc cttcgaccgc atcgaccggt
     4921 cgatcccgct gatctggggc agcatcttcg cccccgacga cgaggagggc cgggtgcggg
     4981 gtcgtcagat ccgcgacttc cacgtcgaca tcaagggcac cgacggccac ggaaagcgct
     5041 accacgctct ggatcccgac gtgtactggt gggcgcacgc cacgttcacc tgggagtact
     5101 tccgggcccg cgagctgttc ttcccctgcc cgctcacccg tcgtcagaag gaccggctct
     5161 acgccgagac cgtcacgtgg taccgccgct acggcgtcag cgaccgtccg gtgccggcca
     5221 cctaccgcga gttccgccac cgcttcgacg agatctgtcg ccacgagctc gagctcaccc
     5281 cggcggtgca gtgggtgctc gaccagtcca ccaacccggc ggccaccgcg gacccggtgc
     5341 gtctgccggg gatgtggtcc gtgctcgacg gtcttgtcac caacctgggg accgagaccc
     5401 tgcgtctgct ggtgtacggg ccgatgcccg acgtcgtgag gcgccgcttc gacctaccgt
     5461 ggtcgcactc ggatcgggtg gcgttcaccg ctctgtgcgc caccatccgg gcgatggacc
     5521 cggccatccg gcgaggggcg ctcgacgcca tctaccccga aggcaccccg cacccggatc
     5581 ggcgtgaccc gacccgcctg gtcgtggcgg gaccgaaccg ccggcagcgg gcgctggccg
     5641 ccgcccgttc cgccgcctga ccgctgctcc cgcgggtctg cttccgtcgg gacagtgacc
     5701 aacgaccctg gcgcgctcgc cgatcggagt tattctagtc cgcggtagca atattgcgga
     5761 tggtccgggt cgcccgtcga ggcgctcgcg cgccgatcga ggagctgtca tgggtgcagt
     5821 cgaaccaacg tcgtcggtga cgaccgcggg cgacctgtcg acccgcaccc agatccacga
     5881 cctggtcgtc gacttctacc gagagatcgt cttcgacgac ctcctcggcc cggtgttcgt
     5941 cgacgtcgcc gaggtcgact gggcggtgca catcccgatc ctggtcgact actggtgccg
     6001 catcgtgttg ggactgcccg gttccaacgc ccgggtcacc gctgtgcacc gtcacgtcca
     6061 cgccctcgag cccctgggcg tcgagcactg cgaccgctgg tacgagctgt ggacggcctg
     6121 cctggacgcc cgctggagcg gaccgctggc cgagagggcc cgaagccacg ccgccgcgct
     6181 gatgtcgggg atggcccgcc acgtgttcga cgtcgagtgg caggcaccga gcgccgcgcc
     6241 cgctggagcg ctctcgtgac tgccaccgat ccgctcgacg acctccagat cgacctgacc
     6301 gaccacgccg cccggtcgcg ggaagaacgc cccgcaacga cggctccggc cagcggcgcg
     6361 gcgcccgtcc cccgtcagcg gtcgatgttg cggtcggtcg ccctgccgag tgagcacggc
     6421 ggttggggcc tgaccggcga gcccatcgtg ctgggcctcc tgctcgcccc gagcctggcc
     6481 ggcctgctcg tcggcctggc ggggatgatc gcgttcgtcg cccgcacccc ggtgtcgctg
     6541 gtgctcgtcg acgggttccg ccaccgcgac ctggagcgca cccggctcgc ccgtcgggtc
     6601 gcggccatcg agatctcggc gctggtcgtc ctggtcgctg gagcgacggc cctggccgga
     6661 cccggcttct ggtggccggt cgcgatcgct gccccgctgg tctcggtgca gctctggttc
     6721 gaccggcgct cccgcagccg ccggctcctg cccgagctgg ccggcgcggt cggcatcacc
     6781 tctctggtgg ccgccatcac cctggccggc ggtggcgaca ccgccgtggc ggtcggagcg
     6841 tgggcgatcc tggccgctcg ggcgctgaca tcgatcccgt tcgtcaagtc gctggtcatg
     6901 aagctgcacg gcaaggcgac ggctcagtgg accctgatca cctgggacgt cgtggccgtc
     6961 gccatcgtgg tcaccgcggc gtggctcgaa ccgacgttga ccgccggtgc gggcgcggtc
     7021 gtggcggtcg tggcgctcca gaggctgacg acgttgcgtc cgccgccgcg cccgacggtg
     7081 acggggatcc gacagacgtt catggggctc gccgtcgtgg tggccacctg gctcggcgtc
     7141 ctgatggggg gagcatgaag aacgacgcag accggtccgg ctcgaccgag ggcatcgacg
     7201 cgggatccgg ttccggcccg gcccgtgtcc tggtcgacgc caccgaatcg ggcgtgcgcg
     7261 tccgggtcca tccgggcctg tgcgagggat ggggcaactg ccaccgttgg agccccgagg
     7321 tgtacccgct cgacgacgag ggctacatcg ccatccacat gctcgaggtc ccgcccgagc
     7381 tcgcccgcct cgcgtggcgg ggagcggacg cgtgcccggc tcaggccatc agcgtgatcg
     7441 gcgagcgtcc gccgccg
//
LOCUS       DAOQOD010000151         7426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3768_length_7426_cov_9.054674, whole genome shotgun sequence.
ACCESSION   DAOQOD010000151 DAOQOD010000000
VERSION     DAOQOD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3768_length_7426_cov_9.054674"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1093)
                     /locus_tag="PLZ72_12730"
     CDS             complement(38..1093)
                     /locus_tag="PLZ72_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNA37208.1"
                     /translation="MNDSPVPTGPVPTGPVPTGPVPNAPVPNAPVPTGDPRPDVLVAR
                     LATTQHGVVTRRQAMALGMTQRMIHRRVETGMWTPVLRGVYSFTGLPDSRTRRLWAAV
                     LHAGDSGVLSLHTAGRLHGFEEIRSTELVYLNVDDVRRHPPEGVVWHRQIDMVPADVV
                     TIDGLPVTSIPRTGMDLAGDPAMSITRLRRFVESALVHRGFEPADFGVVLSRIRRSGK
                     PGVTRMERVLDEVGPGVDLPRSELERLLDRVIERAGLPSPRHEHPLPGAWDRPGFVDR
                     CWPEVRLVVEADGRRWHTRRQQIATDHDRAIDAQAAGYQTTRLLWEHLHGDPEGTARR
                     LRLVYEARLRDVGRAFP"
     gene            complement(1283..2110)
                     /locus_tag="PLZ72_12735"
     CDS             complement(1283..2110)
                     /locus_tag="PLZ72_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNA37209.1"
                     /translation="MVAPQATGPSGEPDRHWFEDIADHVGPAYLRYSFTMGTEQEVGF
                     LVDLLGLEPGMRVLDVGCGPGRHALALARRGIAVLGVDISERFIEVAIEVADREGLSD
                     LARFERADARALVGDPRFDRSLGDTDGAGVGFDAAISLCQGAFGLGGPPDVGDPQNLA
                     ADTEVLRGIAAALRPGGRCAVSAFSSYFQVRWLEESDTFDAAGGVNHERTELRDSDGA
                     RAEADLWTTCYTPRELRLVAERVGLVVDHIWSVTPGGYDMAPPSVDTHEFLLTARTP"
     gene            complement(2116..4833)
                     /gene="polA"
                     /locus_tag="PLZ72_12740"
     CDS             complement(2116..4833)
                     /gene="polA"
                     /locus_tag="PLZ72_12740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNA37210.1"
                     /translation="MPTVMLVDGNSLTYRAFFALPTDLATASGQVTNAVFGFTSMLLN
                     LVRDHRPDQVVITFDRPEPTFRHQRLDTYKANRDSAPDILREQMGLVRQVVATLALPV
                     VEMPGWEADDVIATLTDQAVARGDDVVIVTGDRDSYQLVRDPHVKVLYNRRGVSDYVL
                     YDEAGILERTGVRPEQYVHYAALRGDPSDNLPGVPKVGEKTAARLVSTYGDLDGIFAH
                     TAEQTPALRRNLEENEAQARTNAELMALVHDVPLEVGLGDLDQGDIVADDVLQLFGLL
                     EFPSLVPRLAEAWPELFGDGAAAVPDAPVLEAETAVVDTAADAAAALGRLAALPQVAV
                     AAGWVGTPGRSELTGVALVEDSDAGDVVWIPAELLVGAAGAEPGASLGSAVGSVLCGD
                     PAVVGHDVKPLIRSLLGLGLDLTRLTLDTKLAAYLLDPADTAYSLVDLLRRHTGMELA
                     GAESAGDGATQGQLDLDGTAVAESQRVAREALAVARVAAPLIDSLDAHGLTSLNDSIE
                     VPLVRVLARMEHLGVGVDVDVLRALNASLTEECETERAAIVAAAGEEFNVNSTPQLRT
                     VLFERLGLTPQKKTKTGYSTDQATLEKLQGEHPIIDHLVRYREVEKLRSTYGEGLLAE
                     VAADGRIHATFNQTVARTGRLSSDAPNLHNIPVRSEEGRRFREAFVPAAGHELLVADY
                     NQIELRVIAHLAQDPGLIEAFRTGQDIHTTTAARVFDVDPADVTVEQRSKAKMVSYGL
                     AYGMEAYGLGQRLNIPTADAQVILDAYFAAFPSVKEFMERTVAEARDRGYTETVFGRR
                     RRIPELSSGQRNVRMAGERQAMNAPIQGLAADIFKVALVRLDRAIDERGLGSRLILQV
                     HDEVLVEVPDGELDDAVDVVTHAMSGAFDLSVPLAVNLSHGSSWAAAKG"
     gene            complement(4861..5454)
                     /locus_tag="PLZ72_12745"
     CDS             complement(4861..5454)
                     /locus_tag="PLZ72_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNA37211.1"
                     /translation="MPTTIVVAEDEAIIRMDLTELLQEEGYDVVGECGRGDEAVRLVR
                     ELRPDVALLDIKMPGQDGISAAREISAERLSAVVLVTAFSQRELIEEASDAGVHGYVV
                     KPFERHDLAPAIEVAISRFRAEGLLVDQIASAEDRLAARKVVDRAKGVLMDGHGLSEA
                     DAYSFIQRSAMADRRQMRDVAQDVLDGSLSPRAAESS"
     gene            complement(5534..5812)
                     /locus_tag="PLZ72_12750"
     CDS             complement(5534..5812)
                     /locus_tag="PLZ72_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37212.1"
                     /translation="MPFLVERRLRAVGRRLGALRHELAIADEQLAHFADISDDSRIRS
                     LVSETPLADQEHRDAERTTAAMARHRAELAEQIARLEREQDDLLDRLH"
     gene            5836..>7426
                     /gene="malQ"
                     /locus_tag="PLZ72_12755"
     CDS             5836..>7426
                     /gene="malQ"
                     /locus_tag="PLZ72_12755"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNA37213.1"
                     /translation="MQGRDSYGIADGWWGTDGAWHQASESARSALREAMGGDEHPDGP
                     PDAPPGSPSLWFLRPGEDRSIWSPGVLELEDGTSVPVHDALPADLPIGTHTLRSDGDH
                     VTRVFHLPGPIRRVDRGWGLSVQLPTTRSRASWGHGELADLATLARWTARHGASVLAH
                     NPLGSTIPMLPQQRSPYFASSRRALSPLYLRVEDIAGAERLGDRLNRAANAGRALLDR
                     PTIDRDEVWRIKSEALRELWALVRDDPAGPPEDAGSTGTDTHPFELDHARFAALAERH
                     GGGWSRFPPSARHPRSPALAEALVGLHDDVERWRWVQATCDGQLADAAEAGARVDVEL
                     MADLPVGFDPDGADAWIDQDLLALGCRIGAPPDDLGPLGQDWGLPPYVPWRLRAAGYQ
                     PWIDTLRRLLHHSGLLRIDHVMGLFRLYCIPPGHDALDGAYVYSHGAELLDLAVMEAA
                     RAGAVLVGEDLGTVEPEVRDAMAERGVYGYAVGWFADEDPTTWPPRTVAMLGTHDLPT
                     AAGLWDGTDAADRRAAGLPDSP"
BASE COUNT         1032 a         2610 c         2749 g         1035 t
ORIGIN      
        1 ttctgacgac agtcacgaca ctggcggtac tcaactgtca tgggaatgcc cgcccgacgt
       61 cgcggaggcg ggcctcgtag acgagccgca gacgacgggc cgtcccctcg ggatcgccgt
      121 ggaggtgctc ccacagcagg cgggtggtct ggtagccggc ggcctgggcg tcgatggccc
      181 ggtcgtggtc ggtcgcgatc tgctggcgac gggtgtgcca gcgccgaccg tcggcctcga
      241 cgacgagtcg gacctcgggc cagcaccggt cgacgaatcc cggacgatcc cacgcccccg
      301 gcagggggtg ctcgtggcgc ggcgagggaa gcccggcacg ctcgatcacc cggtcgagga
      361 gccgttcgag ctcgctgcgg ggcaggtcga cgccgggccc gacctcgtcg agcacccgtt
      421 ccatgcgcgt cacgccgggc ttgccgcttc gccggatcct cgacaggacc actccgaagt
      481 cagcaggttc gaacccccgg tgcaccaggg ccgactcgac gaagcgccgc agccgggtga
      541 tggacatcgc cgggtcaccg gccagatcca tgccggtgcg gggtatcgag gtcacgggca
      601 ggccgtcgat cgtgacgaca tccgcgggca ccatgtcgat ctgacggtgc cacacgaccc
      661 cctccggcgg atgccgacgg acatcgtcga cgttgaggta caccagctcg gtcgagcgga
      721 tctcctcgaa gccgtggagg cgacccgcgg tgtgcaacga cagcaccccg ctgtcgccgg
      781 cgtggagcac cgccgcccac agccgtcggg tccggctgtc gggaaggccg gtgaacgagt
      841 agacgccccg gagcaccggc gtccacatcc cggtctcgac gcggcggtgg atcatgcgct
      901 gggtcatgcc caacgccatc gcctgtcgcc gggtgaccac cccgtgctgt gtggtggcca
      961 gacgggcgac caggacgtcg ggacggggat ccccggtcgg gacaggcgcg ttcgggacag
     1021 gcgcgttcgg gacaggaccg gtcgggacag gaccggtcgg gacaggaccg gtcgggacag
     1081 gactgtcgtt catcgcgcca cgttgcgcca tggacgggcc ggtgtcaccg gggaccgatc
     1141 cccgttgcgc ggccaacgga ggtgtgctcg ggccaccccg gcggattctc gtgacagttg
     1201 agtaccggcc gtgtcgtgac ttgtcgtcag aacgcgcggg tcagaacgcg cgggtcagaa
     1261 cgcgggggtc agaacgcggg ggtcaggggg tgcgggcggt gaggaggaac tcgtgggtgt
     1321 cgacgctggg gggcgccatg tcgtagccgc cgggcgtgac cgaccagatg tggtcgacca
     1381 ccagcccgac ccgctcggcg accaggcgca gctcgcgggg tgtgtagcag gtggtccaca
     1441 ggtcggcctc ggcccgcgcc ccgtcggagt cgcgcagctc ggtccgctcg tggttgaccc
     1501 ctccggcggc gtcgaaggtg tcggactcct ccaaccaccg gacctggaag tacgacgaga
     1561 acgccgagac ggcgcagcgc ccaccgggcc gcagggcagc agcgatgccc cgcagcacct
     1621 cggtgtcggc ggcgaggttc tgcgggtctc ccacatccgg cggtccaccc agcccgaacg
     1681 cgccctggca cagcgagatg gcggcgtcga agccgacccc ggcgccgtcg gtgtcgccga
     1741 gcgaccggtc gaagcggggg tcgccgacca gggcacgtgc atcggctcgc tcgaagcggg
     1801 ccaggtccga caggccctcg cggtcggcga cctcgatggc gacctcgatg aaccgctcgc
     1861 tgatgtcgac gccgagcacc gcgatgccgc gccgggccag cgccagggcg tgccgtcccg
     1921 gtccgcagcc gacatccagc acccgcatac ccggttccag gccgaggagg tcgaccagga
     1981 agccgacctc ctgctcggtg cccatggtga acgagtagcg caggtaggcg ggaccgacgt
     2041 gatcggcgat gtcctcgaac cagtgccgat ccggctcgcc ggacggtccg gtggcctggg
     2101 gagcgaccac cgtgctcagc ccttggccgc ggcccagctg gagccgtgcg agaggttgac
     2161 cgcgaggggg accgacaggt cgaaggcgcc cgacatggcg tgggtgacga cgtcgaccgc
     2221 gtcgtcgagc tcgccgtcgg ggacctcgac gagcacctcg tcgtgcacct ggaggatcag
     2281 ccgactgccc aggccacgct cgtcgatcgc ccggtcgagg cggacgagcg ccaccttgaa
     2341 gatgtcggcg gccagaccct ggatcggggc gttcatggcc tggcgctccc ccgccatgcg
     2401 cacgttgcgc tgaccggagc tcagctcggg gatacgtcga cgacgaccga acaccgtctc
     2461 ggtgtagccc cggtcgcggg cctcggccac ggtgcgctcc atgaactcct tcaccgacgg
     2521 gaacgccgcg aagtaggcgt cgaggatcac ctgggcatcc gcggtgggga tgttgaggcg
     2581 ctgaccgagc ccgtaggcct ccatgccgta ggccaacccg taggagacca tcttggcctt
     2641 ggagcgctgc tcgacggtga cgtcggccgg atcgacgtcg aagacccgcg ctgcggtggt
     2701 ggtgtggatg tcctggccgg tgcggaacgc ctcgatcagc cccggatcct gggccaggtg
     2761 ggcgatgacg cgcagctcga tctggttgta gtcagcgacg agcagctcgt ggcccgccgc
     2821 gggtacgaac gcctcgcgga agcgccgccc ctcctcgctg cgcaccggga tgttgtgcag
     2881 gttgggggcg tcggaggaca gccgaccggt ccgggcgacg gtctggttga acgtggcgtg
     2941 gatccgacca tcggcggcga cctcggcgag caggccctcg ccgtaggtgg agcggagctt
     3001 ctcgacctcg cggtagcgca cgaggtggtc gataatcggg tgctcgccct ggagcttctc
     3061 gagcgtggcc tggtcggtgg agtagccggt cttggtcttc ttctgcgggg tgaggccgag
     3121 ccgttcgaac agcacggtgc gcagctgcgg ggtggagttc acgttgaact cctcgcccgc
     3181 ggcggcgacg atcgcggccc gttcggtctc gcactcctcg gtgagcgagg cgttgagcgc
     3241 ccgcaggacg tccacgtcga cgccgacgcc gaggtgctcc atgcgggcca gcacgcgcac
     3301 cagcggcacc tcgatggagt cgttgagcga cgtgagcccg tgggcgtcga gggagtcgat
     3361 cagcggagcg gccacccggg ccaccgccag cgcctcacgg gcgacccgct gcgactcggc
     3421 caccgcggtg ccgtcgaggt cgagctgtcc ctgcgtcgca ccgtcgccgg ccgactcggc
     3481 tccggcgagc tccatgccgg tgtggcgacg cagcaggtcg accagcgagt aggcggtgtc
     3541 ggccgggtcg agcaggtagg cggccagctt ggtgtccagc gtcaggcggg tgaggtcgag
     3601 tccgaggccg agcagggagc ggatcagcgg cttcacgtcg tggcccacca ctgcggggtc
     3661 gccgcacagg accgatccga cggcggatcc gagcgacgct ccgggttctg ctccggccgc
     3721 ccccaccagc agctccgcgg ggatccacac cacgtcgccg gcgtcggagt cctcgaccag
     3781 cgcgacgccg gtcagctccg accgacccgg tgtcccgacc cagccggcgg cgaccgcgac
     3841 ctgcgggagc gccgccaggc gacccagagc cgcggcagcg tcggccgcgg tgtcgacgac
     3901 ggcggtctcg gcctcgagca ccggcgcgtc gggcaccgcg gcggccccgt cgccgaacag
     3961 ctcgggccac gcctctgcca ggcgcgggac caggctgggg aactcgagca ggccgaacag
     4021 ctgcagcacg tcgtcggcga cgatgtcgcc ctggtccagg tcgcccaggc cgacctccaa
     4081 cggcacgtcg tgcaccaacg ccatcagctc ggcgttggtg cgggcctgcg cctcgttctc
     4141 ctcgaggttg cggcgcagcg ccggggtctg ctcggcggtg tgggcgaaga tcccgtcgag
     4201 gtcgccgtag gtgctgacca gccgggcggc ggtcttctcg cccaccttgg gcacgccggg
     4261 caggttgtcg gacgggtcgc cgcgcagagc ggcgtagtgg acgtactgct cgggccggac
     4321 cccggtgcgc tcgaggatcc ccgcctcgtc gtagaggacg tagtccgaca cgccgcgacg
     4381 gttgtagagc accttgacgt gcgggtcgcg caccagctgg tagctgtcgc ggtcgccggt
     4441 gacgatgacg acgtcgtcgc cgcgtgccac cgcctggtcg gtcagggtgg cgatcacgtc
     4501 gtcggcctcc cacccgggca tctccaccac cggcagcgcc agggtcgcca cgacctggcg
     4561 caccaggccc atctgctcgc ggaggatgtc gggggcggag tcgcggttgg ccttgtaggt
     4621 gtcgagccgc tggtggcgga acgtcggttc gggccgatcg aaggtgatca ccacctgatc
     4681 gggccggtgg tcgcgcacca ggttgagcag catcgaggtg aacccgaaca ccgcgttggt
     4741 cacctgaccc gaagcggtgg ccagatcggt cgggagggcg aagaacgccc ggtaggtcag
     4801 cgagttcccg tcgacgagca tcacggtcgg caccggcgcg accctaggcg gtcaccccga
     4861 tcagctcgac tcggcggcgc gcgggctcaa cgagccgtcg agcacgtcct gggccacgtc
     4921 gcgcatctga cgtcggtcgg ccatggccga gcgctggatg aacgagtagg cgtcggcctc
     4981 gctcaacccg tggccgtcca tcaggacgcc cttggcccgg tcgaccacct tgcgggcggc
     5041 caggcggtcc tcggccgacg cgatctggtc gaccagcagt ccctcggccc ggaaccgcga
     5101 gatggcgacc tcgatggcgg gggccaggtc gtggcgctcg aacggcttga cgacgtatcc
     5161 gtgcacgccg gcgtcgctgg cctcctcgat gagctcgcgc tgggagaagg cggtgaccag
     5221 cacgacggcg gacaggcgct cggcggagat ctcacgggcc gccgagatgc cgtcctggcc
     5281 gggcatcttg atgtcgagca gggcgacgtc gggccgcagc tcccgcacca ggcggaccgc
     5341 ctcgtcaccg cggccgcact caccgacgac gtcgtagccc tcttcctgga gcagctcggt
     5401 caggtccatc cggatgatgg cctcgtcctc tgcgaccacg atcgtggttg gcaccgttgc
     5461 ccaccgtcct cgtcttcgta tcggccggcc gggtcgccgg agactgaccc ggcgacgttc
     5521 acaccgggag gcgtcagtgt aggcggtcga gcaggtcgtc ctgctcccgc tccagccggg
     5581 cgatctgctc cgccagctcg gcccggtgcc gggccatcgc cgcggtcgtc cgctcggcgt
     5641 cgcggtgctc ctggtccgcg agcggcgtct cggacaccag cgacctgatg cgggaatcgt
     5701 cggagatgtc ggcgaagtgg gccagctgct cgtccgcgat cgccagctcg tgccggagcg
     5761 cgcccagccg tcgtcccacg gcgcggaggc ggcgttcgac caggaacggc atggtcgcag
     5821 tgtagaaacg ccggggtgca gggacgtgac agctacggga tcgcggacgg ctggtggggc
     5881 accgacgggg cgtggcacca ggcgtcggag tcggcccgct cggcgctgcg ggaggcgatg
     5941 ggcggcgacg agcaccccga cgggcctccc gacgctccgc ccggctcccc gtcgctgtgg
     6001 ttcctgcgac ccggcgagga ccgctcgatc tggtccccgg gcgtgctcga gctcgaggac
     6061 gggacgtcgg tgccggtgca cgacgccctg ccggcggacc tgccgatcgg gacccacacg
     6121 ctgcgctccg acggcgacca cgtcacccgg gtgttccacc ttcccggtcc gatccggcgc
     6181 gtcgatcggg ggtgggggct gtcggttcag ctgccgacga cccggtcccg cgccagctgg
     6241 ggacacggcg agttggccga cctggccacc ctggcgcggt ggacagcccg acacggcgcg
     6301 tcggtgctgg cccacaaccc gctcgggtcg accatcccga tgctccccca gcagcgctcg
     6361 ccgtacttcg cctcgtcgcg acgggcgctg tcgccgctgt acctgcgcgt cgaggacatc
     6421 gccggcgcgg agcgcctcgg cgaccgcctg aaccgggcgg ccaacgcggg ccgcgccctg
     6481 ctggaccgcc cgacgatcga ccgcgacgag gtgtggcgca tcaagtcaga ggccctgcgg
     6541 gagctgtggg cgctggtccg cgacgatccg gcggggccac ccgaggacgc cgggagcacc
     6601 gggaccgaca cacacccgtt cgagctcgac cacgctcgat tcgcggccct ggccgaacga
     6661 cacggcggag ggtggtcgcg gttcccgccg tcggcccgac atccccgcag ccccgctctc
     6721 gccgaggctc tggtcggact gcacgacgac gtcgagcggt ggcggtgggt ccaggccacc
     6781 tgcgacggcc agctggccga cgcggccgag gcaggcgcac gggtggacgt cgagctgatg
     6841 gcggacctgc ccgtcggctt cgaccccgac ggcgcggatg cctggatcga ccaggacctg
     6901 ctcgcactgg gctgtcggat cggggccccg cccgacgacc tcggtccgtt gggccaggac
     6961 tggggactgc cgccgtacgt gccgtggcgg ttgcgcgccg cgggctacca gccgtggatc
     7021 gacaccctgc gtcggctgct tcaccacagc ggtctgctgc gcatcgacca cgtcatgggc
     7081 ctgttccggc tctactgcat cccgcccggc cacgacgccc tcgacggcgc gtatgtgtac
     7141 tcccacggtg ccgagctgct ggacctggcg gtgatggagg cggcccgggc cggagcggtg
     7201 ctcgtcggcg aggacctggg caccgtcgag cccgaggtgc gcgacgccat ggccgagcgc
     7261 ggcgtgtacg gctacgccgt cggttggttc gccgacgagg acccgacgac gtggccgccg
     7321 cgcaccgtgg cgatgctcgg cacccacgac ctgcccaccg cggccggtct gtgggacggc
     7381 accgacgccg cggatcgccg cgccgccgga ctgccggact cacccg
//
LOCUS       DAOQOD010000152         7383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3792_length_7383_cov_12.942822, whole genome shotgun sequence.
ACCESSION   DAOQOD010000152 DAOQOD010000000
VERSION     DAOQOD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_7383_cov_12.942822"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLZ72_12760"
     CDS             <1..369
                     /locus_tag="PLZ72_12760"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNA37214.1"
                     /translation="CGRDDDSARIVTSRVVDVELPGFARKVLSPTNTMVQTDEWRRDG
                     DGWTGEFRVEVAGAPVEMSGTMSLEPDASGTVHTVTTSMKVKVPLVGGKIADWAAKND
                     IPRTLQAEFDANDDWLSSHG"
     gene            complement(472..2004)
                     /locus_tag="PLZ72_12765"
     CDS             complement(472..2004)
                     /locus_tag="PLZ72_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNA37215.1"
                     /translation="MAHDPVVAETTAVAENLEPRFVIEDQEALAAAALARYHSTGRRP
                     NILMVLFDDVGWGDFGCYGGGVAVGAPTPNIDRLARRGLQLTSCYSEPSCTPSRASFM
                     TGRLPLRHGLLRPPMYGQPGGLDGEVTLAELLSAAGYVTQGVGKWHIGENVESQPQNV
                     GFDDFYGFLSVSDMYTEWRDPYFFPEIVYSEERTRWVENLPFNKCFVHGTRGGELEPV
                     EEVTIPVLSLLDEKWANYSLDFIRRMAEPEPEERAPWFLYHCTRGAHFDNYPHERFLG
                     SSPAKHPYKDTIIELDDIVGRLVAELEATGQMDDTIIFISSDNGPEMETWPDAAYSPF
                     RSAKGSTWEGGQRVPGIVVWPGMIEADRASDGVFSLMDLFNTFLHLAGAEDSIPTDRF
                     IDGVDQTSFLLSPDGVSNRKYLYYWLTNVFSGLRVGEWKFMIASSSDDDRDCLNLGGF
                     TGVTQKYSHGRLYNLYLDPKEQHSYLIRKLAYLDSFTSGIAAHVGSFVSHPPKQPIAA
                     MS"
     gene            2146..3831
                     /locus_tag="PLZ72_12770"
     CDS             2146..3831
                     /locus_tag="PLZ72_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37216.1"
                     /translation="MVETWDVVVVGSGPGGLTAGACLAAAGRRVLVLERHDLAGGNCQ
                     VFRRRHLGQDYEFDVGLHYIGDCGPGGLFPSIFGALGVGDRIEFRPLDPDGFDTLVFP
                     DLRLAVPADWDEYRDRVVAALPDERAGVQRCMDILRQVAEESRIRAIPGVDTPTFDRW
                     AFRPLSELFEHAELSARARAVLDHWSGLYAGGPSQTAVVMHATIIDHYMRGAAYPAGG
                     GQVLPARLTQVIEACGGEIRSLSTVEEILVQSGRARGVRLADGSTISAPLVISNADHT
                     RTVQQLVDRRHWDPATLEWVEQATMTLGLVCVYVIVDVELDGPNTNYFLFPSYETDEL
                     YDRLDRGEMPEGVTFAYVAMASRKDPDNPHLCPPGHTNFQIMTLAPRGWEWWGLDDGP
                     ADGARYRRNDSYRGRKADITERLIDAAEQVLGPFRDHIVHVETASPASQERYTHSTGG
                     TSYGYLHSPDQVGERRPAHRTEIDGLWVVGAGTASGHGIAGTMVGGINCAGEILERPL
                     LVEMVMGTTLLEPSAVPADPPDFDPVEWCRGAALRERRAAGRAARAARTAGGT"
     gene            3973..4242
                     /locus_tag="PLZ72_12775"
     CDS             3973..4242
                     /locus_tag="PLZ72_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNA37217.1"
                     /translation="MHRKDEYLKRLRRIEGQARGLQRMVEEDQYCIDILTQVSAMTKA
                     LQAVSLGLLEEHLGHCVLNAARAGGEGADLKLKEASDAIARLVRS"
     gene            4345..4554
                     /locus_tag="PLZ72_12780"
     CDS             4345..4554
                     /locus_tag="PLZ72_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNA37218.1"
                     /translation="MNTSTYTVTGMTCGHCVMSVTEEVSEVPGVTGVDVDLDSGRMTV
                     SSDAPVDDQLVRAAVTEAGYQVADA"
     gene            4840..7191
                     /locus_tag="PLZ72_12785"
     CDS             4840..7191
                     /locus_tag="PLZ72_12785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNA37219.1"
                     /translation="MSALDDDTRAPEADHVLELAIGGMTCASCANRIERKLNKLEGVT
                     ATVNYATEKAKVTFDDSITPEVLVGAVEQAGYSARLPTPPAPTGTAGSAQASDVDEDT
                     PVRALRQRLIISTVLSVPVIAMAMVPALQFTNWQWLSLTLAAPVVVWGAYPFHRAAWT
                     NLRHGTTTMDTLISIGTLAAFGWSLYALFFGTAGEPGMTHAFELTIARTDGAANIYLE
                     AAAGVTTFILAGRYFEVRSKRRAGAALRALLELGAKDVAVLRDGREERIAIDQLAVGD
                     HFVVRPGEKIATDGTIVEGTSAVDASMVTGESVPVEVGPGDAVVGATVNAGGRLVVAA
                     TRVGADTQLAQMARLVEDAQNGKAQAQRLADRISGIFVPIVIALAVATFGFWAGTGDG
                     LTAAFTAAVSVLIIACPCALGLATPTALMVGTGRGAQLGILIKGPEVLETTRRVDTVV
                     LDKTGTVTTGRMTLLEVVVAPGEDTEQVLRLAGALEDASEHPIATAISRGARERVGDL
                     PTVEDFANVAGLGVQGIVNAGADPGSDGAPAVSHAVLVGRPALLEQWSQHLGPELVSA
                     MERSAEQGRTAVAVGWDGRARGVLVVADAVKPTSAEAIAELRGLGLTPVLLTGDNATV
                     ARSVAASVGIDPGPDTVIAEVLPADKVDVVRRLQAEGRSVAMVGDGVNDAAALAQADL
                     GLAMGTGTDVAIEASDITLVRGDLRVAADAIRLARRTLGTIKGNLFWAFAYNVAALPL
                     AAAGLLNPMLAGAAMAFSSVFVVSNSLRLRRFSPARRSGTTAA"
BASE COUNT         1108 a         2633 c         2594 g         1048 t
ORIGIN      
        1 tgcggccgcg acgacgactc ggcgcgcatc gtgacctccc gcgtcgtcga cgtggagttg
       61 ccgggcttcg cccgcaaggt gctgtcgccc accaacacga tggtccagac cgacgagtgg
      121 cgccgcgacg gcgacggttg gaccggcgag ttccgcgtcg aggtcgccgg cgctccggtc
      181 gagatgtcgg ggacgatgtc gctggagccc gacgcctcgg ggaccgtgca cacggtgacg
      241 acgtcgatga aggtcaaggt gccgctcgtc ggcgggaaga tcgccgactg ggccgccaag
      301 aacgacatcc cccggacgct gcaggccgag ttcgacgcga acgacgattg gctctcgagc
      361 cacggctgac cgcacgacca cccgcggcca cccgtcacgc cgattctcat gacaactgag
      421 taccgccagt gtcgtgactt gtcgtcagaa accgggggtg gagggggtgg ctcagctcat
      481 ggcggcgatg ggctgcttgg gcgggtggct cacgaaggat ccgacgtgcg cggcgatgcc
      541 gctggtgaag ctgtcgaggt acgccagctt gcggatcagg taactgtgct gctccttggg
      601 gtccaggtac aggttgtaga gccgaccgtg gctgtacttc tgggtcaccc cggtgaagcc
      661 gcccaggttc aggcagtcgc ggtcgtcgtc ggagctggag gcgatcatga acttccactc
      721 ccccacccgt agacccgaga acacgttggt caaccagtag tagaggtact tgcggttgga
      781 cacgccgtcc ggcgacagca ggaacgacgt ctggtccacc ccgtcgatga accggtcggt
      841 cgggatggag tcctcggctc cggccaggtg caggaacgtg ttgaacaggt ccatcagcga
      901 gaagaccccg tccgaggcgc ggtccgcctc gatcatcccg ggccacacca cgatgcccgg
      961 cacgcgctgg ccgccctccc aggtcgagcc cttggcgctg cggaacggcg agtacgcggc
     1021 gtcgggccag gtctccatct cgggtccgtt gtcggaggag atgaagatga tcgtgtcgtc
     1081 catctgcccg gtcgcctcga gctcggcgac gagccgcccg acgatgtcgt cgagctcgat
     1141 gatcgtgtcc ttgtaggggt gcttggccgg cgacgaaccg aggaagcgct cgtgggggta
     1201 gttgtcgaag tgggcgcccc gggtgcagtg atagaggaac cacggcgccc gttcctccgg
     1261 ctcgggctcg gccattcggc ggatgaagtc gagcgagtag ttcgcccact tctcgtcgag
     1321 cagcgacagg accgggatgg tcacctcttc caccggctcc agctcaccgc cccgggtgcc
     1381 gtggacgaag cacttgttga aggggaggtt ctcgacccag cgggtgcgct cctcgctgta
     1441 gacgatctcg gggaagaagt acgggtcgcg ccactcggtg tacatgtccg acaccgacag
     1501 gaagccgtag aagtcgtcga agccgacgtt ctggggctgg gattccacgt tctcgccgat
     1561 gtgccacttg ccgacgccct gggtgacgta acccgccgcc gacagcagct cggcaagcgt
     1621 gacctcgccg tcgaggccgc ccggctggcc gtacatcggc ggtcggagca ggccgtgacg
     1681 caagggcagc cggccggtca tgaacgacgc ccgcgacggc gtgcacgacg gctcggagta
     1741 gcacgacgtg agctgcaggc cgcggcgtgc cagccggtcg atgttcgggg tgggagcgcc
     1801 gacggcgacg ccgccgccgt agcagccgaa atcaccccag cccacgtcgt cgaacaggac
     1861 catcaggatg ttggggcgac gaccggtcga gtggtatcgg gccagagcgg cagccgcgag
     1921 ggcctcctgg tcctcgatga cgaacctcgg ctcgaggttc tcggccaccg ccgtcgtctc
     1981 agccaccacc gggtcgtgag ccacgtcgtc cccctggatc cgcgggcggc gcccccgtgg
     2041 cgccgctccc gatgggttgg accataccca ccgccactcc ggcgcgccgg gccgcgggtc
     2101 cggttagcgt cgacggatca ccgatcgcga gtcgcggagg aaccgatggt cgagacctgg
     2161 gacgtcgtgg tggtcggctc cggccccggc ggtctgaccg ccggggcgtg cctggccgct
     2221 gccggtcgtc gggtgctggt gctcgagcgc cacgacctgg ccggcggcaa ctgccaggtg
     2281 ttccgccgcc gccatctcgg ccaggactac gagttcgacg tcgggctgca ctacatcggc
     2341 gactgcggac ccggcgggct gttcccgtcg atcttcggcg cgctcggcgt cggcgaccgc
     2401 atcgagttcc ggccgctcga ccccgacggc ttcgacacct tggtgttccc cgacctgcgc
     2461 ctggcggtgc ccgccgactg ggacgagtac cgcgatcgcg tcgtcgccgc gctccccgac
     2521 gagcgtgccg gggtgcagcg ctgcatggac atcctccgcc aggtcgccga ggagagccgg
     2581 atccgggcca tccccggcgt cgacacgccg acgttcgacc gctgggcgtt ccgtccgctg
     2641 tcggagctgt tcgagcacgc cgagctgtca gctcgggccc gggcggtgct cgaccactgg
     2701 tcggggctgt acgccggcgg cccgtcgcag accgcggtgg tgatgcacgc cacgatcatc
     2761 gaccactaca tgcgcggcgc ggcctacccc gcggggggcg ggcaggttct cccggcccgg
     2821 ctcacccagg tgatcgaggc gtgcgggggc gagatccggt ccctgagcac cgtcgaggag
     2881 atcctggtgc agagcggtcg agcccgaggc gtgcgcctgg ccgacggatc cacgatctcc
     2941 gccccgctgg tcatctccaa cgcggaccac acccgcactg tgcagcaact ggtcgaccgg
     3001 cgtcactggg atccggccac actcgagtgg gtcgagcagg cgaccatgac cctggggttg
     3061 gtgtgcgtgt acgtgatcgt cgacgtggag ctcgacggac cgaacaccaa ctacttcctg
     3121 ttcccctcct acgagaccga tgagctctac gaccggctcg accggggcga gatgcccgag
     3181 ggggttacgt tcgcctacgt cgcgatggcg tcgcggaagg accccgacaa cccgcacctg
     3241 tgccccccgg gccacaccaa cttccagatc atgaccctgg ccccccgggg ctgggagtgg
     3301 tggggcctcg acgacggccc cgcggacggc gcccgctacc gccgcaacga ctcctaccgc
     3361 ggccgcaagg ccgacatcac cgagcggctg atcgacgcgg cggagcaggt gctcggcccg
     3421 ttccgcgacc acatcgtgca cgtcgagacg gccagccccg cctcccagga gcgctacacc
     3481 cactccaccg gtgggacgag ctacggctac ctccactccc ccgaccaggt gggagaacgc
     3541 cggccggcgc accgcaccga gatcgacggg ctgtgggtgg tgggggcggg cacggcgtca
     3601 ggccacggca tcgccggcac gatggtcggc gggatcaact gcgcgggcga gatcctggag
     3661 cggccactgc tggtcgagat ggtgatgggc accacgctgc tggagccctc ggcggtacct
     3721 gcggatccac ccgacttcga tccggtggag tggtgtcggg gggctgcgct gcgtgagcgc
     3781 cgggccgcgg gccgagcggc ccgcgctgcc cgcacggccg gaggcacctg aacttcctct
     3841 tgcttggtac cctacggggg tatagtctac cttcagttcg agacggaccg agggagcatg
     3901 accgaccaca ccaccacccc gaccgagacc agcgacaccg gttgccgcga ccacgatccg
     3961 ggccccggct acatgcaccg caaggacgag tacctcaagc ggctgcgccg gatcgagggc
     4021 caggcccggg gactgcagcg catggtcgag gaggaccagt actgcatcga catcctcacg
     4081 caggtgtcgg ccatgaccaa ggccctgcag gcggtgtcgc tcggactgct cgaggagcac
     4141 ctcggccact gcgtgctgaa cgcagcacgg gccggcggcg agggggcgga cctgaagctc
     4201 aaggaggcat ccgacgccat cgcccgcctg gtccgctcct gatcgggccg gcggtccacc
     4261 ctgctgcgga tccccgcacc ccgatcacgc acagcgacac ccatcaccca cgacccccat
     4321 ccctcacgac acggaggaga caccatgaac accagcacct acaccgtcac cggcatgacc
     4381 tgcggccact gcgtgatgtc ggtcaccgag gaggtcagcg aggtcccggg cgtcaccggc
     4441 gtcgacgtcg acctggactc cggccggatg accgtctcct ccgacgcacc ggtcgacgac
     4501 cagctcgtcc gcgccgcggt gaccgaggcg ggctaccagg tcgccgacgc ctgagcgtcg
     4561 gcgacctgag ccacggatcg gagctgtgat gtccaccccg gccaggatca ccgccttcct
     4621 cgcagggctg gtggtcgtgt tcctgttgtc gttcgccatc gggcgggcgg tcgggcccac
     4681 agcgggcccg accgcaccga cccatggcgg aggccacccg accacgacct ccacgacccc
     4741 ctcgccgacc gtgaacgccc acgacacggg cggccacgga ggctgagcgg cacccgccgc
     4801 gtcacacccg tccgccctca cgaccgagag gagcccccga tgagcgcact cgacgacgac
     4861 acccgagcac ccgaggccga tcacgtcctg gagctggcga tcggcggcat gacctgcgcc
     4921 tcgtgcgcca accggatcga gcgcaagctc aacaagctcg agggcgtgac cgcgacggtc
     4981 aactacgcga ccgagaaggc caaggtcacc ttcgacgact cgatcacccc cgaggtcctc
     5041 gtcggcgcgg tcgagcaggc gggctactcg gcccgcctgc ccacaccgcc ggcaccgacg
     5101 ggcaccgccg gcagcgcaca ggcgtccgac gtcgacgagg acacgcccgt ccgggcactt
     5161 cgccaacgcc tgatcatctc gacggtcctg agcgtgccgg tcatcgccat ggcgatggtc
     5221 ccggcgctgc agttcaccaa ctggcagtgg ctgtcgctca ccctggccgc accggtggtg
     5281 gtgtggggcg cctacccctt ccaccgcgcc gcgtggacca acctccggca cggcaccacc
     5341 acgatggaca cgctgatctc catcggcacc ctcgccgcgt tcggctggtc gctctacgcc
     5401 ctgttcttcg ggaccgccgg cgaacccggg atgacgcacg ccttcgagct caccatcgcc
     5461 cgcaccgacg gggccgccaa catctacctg gaggccgcgg ccggggtcac cacgttcatc
     5521 ctggccggtc gctactttga ggtccgctcc aagcggcggg caggagcggc gctgcgggcg
     5581 ctgctcgagc tcggcgccaa ggatgtcgcc gtgctgcgcg acggacgcga ggagcgcatc
     5641 gccatcgacc agctcgccgt cggcgaccac ttcgtggtgc gccccggcga gaagatcgcc
     5701 accgacggca cgatcgtcga gggcacctca gcggtcgacg cgtcgatggt caccggcgag
     5761 tcggtgccgg tggaggtcgg ccccggagac gcggtggtgg gcgcgacggt caacgccggc
     5821 ggacggctgg tggtggcggc gacgagggtc ggcgccgaca cgcagctcgc ccagatggcc
     5881 cgcctggtcg aggacgcgca gaacggcaag gcccaggccc agcggctggc ggatcggatc
     5941 tcgggcatct tcgtgccgat cgtgatcgcg ctggccgtcg cgacgttcgg gttctgggcc
     6001 ggcaccggcg acgggctcac cgcggcgttc accgccgcgg tgtcggtgct gatcatcgcc
     6061 tgcccgtgcg ccctgggtct ggcgaccccg accgcactga tggtgggaac cggccgcggc
     6121 gcccagctcg gcatcctgat caagggcccc gaggtgctcg agaccacccg ccgggtcgac
     6181 accgtcgtgt tggacaagac cggcacggtc accaccgggc gcatgacgct gctcgaggtg
     6241 gtcgtggccc ccggcgagga caccgagcag gtgctgcggc tggccggagc gctcgaggac
     6301 gcgtcggagc acccgatcgc caccgccatc tcgcgcggcg cccgcgagcg agtcggcgac
     6361 ctgccgacgg tcgaggactt cgccaacgtc gccggcctcg gcgtccaggg catcgtcaac
     6421 gccggggcag atcccgggtc cgacggggct ccggccgtct cccatgccgt gctcgtcggg
     6481 cgccctgcac tgctcgagca gtggtcccag cacctgggac cggagctggt gtcggccatg
     6541 gagcgctccg cggaacaggg acgcaccgcg gtggcggtcg gctgggacgg tcgggcccgc
     6601 ggcgtgctgg tggtcgccga cgcggtgaag ccgacctcgg ccgaggcgat cgcggagctg
     6661 cgcggactgg gcctcacccc ggtgctgctc accggcgaca acgccaccgt cgctcgctcg
     6721 gtggccgcct cggtcgggat cgaccccggc cccgacaccg tcatcgccga ggtgctgccg
     6781 gctgacaagg tcgacgtggt gcgtcggctc caggccgagg gccggtcggt ggcgatggtg
     6841 ggcgacggcg tgaacgacgc cgcggcgctg gctcaggccg atctgggcct ggcgatggga
     6901 acgggcaccg acgtcgccat cgaggcgtcc gacatcaccc tggtgcgcgg cgacctgcgg
     6961 gtcgcggccg acgccatccg gctggcccgg cgcacgctcg gcaccatcaa gggcaacctg
     7021 ttctgggcgt tcgcctacaa cgtggcggcg ctcccgctgg ccgccgcggg cctgctcaac
     7081 ccgatgctgg cgggagcggc gatggcgttc tcgtcggtgt tcgtggtgtc gaacagcctg
     7141 aggctgcgcc gcttctcgcc tgcccgtcga tcgggcacga cggcggcatg atccgtcccc
     7201 gccgggctcg gggacaccgg cggggacgga tcagatcatg tcgacgggcc cgagcccgcg
     7261 tcgtacggat cggatccgga tcgggctcag accgacgagg cgcccgcggc ggcctcgtgc
     7321 tcggcctcga gcttggactc gtgcagcacg aaccccatca tcccgccgaa gccgggaccg
     7381 ccc
//
LOCUS       DAOQOD010000153         7297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3858_length_7297_cov_11.120547, whole genome shotgun sequence.
ACCESSION   DAOQOD010000153 DAOQOD010000000
VERSION     DAOQOD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3858_length_7297_cov_11.120547"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLZ72_12790"
     CDS             complement(<1..656)
                     /locus_tag="PLZ72_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37220.1"
                     /translation="MKTSLPRSSRPVRTARAARSVAVVASVALVVGAGLTGTASAQDV
                     EGTRAKVQRLAAEMDRLEARASELDEQYLQTGIELSKAETELKKNRDAVADAQARMDQ
                     ARSEANSYIVSAYMSAGSDVAGLGVGDPNVAVNEKVLMETLHGDRQQVADDLRAAQMD
                     LDDRSADLEQASKALEDKKARQSSIKADLEASVSAQQDLLDGANSELRAAIAAEQERQ
                     A"
     gene            850..1611
                     /locus_tag="PLZ72_12795"
     CDS             850..1611
                     /locus_tag="PLZ72_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37221.1"
                     /translation="MTDLTSPQNPTAASTAAPTAGPAPGAPTDPSFDGGPRPRPTGPP
                     TRWGRPDVTEWLVARRNAGATPAVIATELVARGWDADAAADAARRSLRRSDHHRVLYG
                     TLCWSVGLGALGLATGLHQLLADTPDPEWAALAFTLAVVLLPLAGWCLWSAQQVEASS
                     AHAVWSPTRRMWFATLAACTAIIGLIRLITYVYLVIAAMTGARSTPLTPDDQFQVLIS
                     LAIVVPLFVWSFHEWRRSDVVLSGLKELDGSEGGR"
     gene            1608..2816
                     /locus_tag="PLZ72_12800"
     CDS             1608..2816
                     /locus_tag="PLZ72_12800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNA37222.1"
                     /translation="MRPAPTVTPGRAPAVAPAVAPAVAPGRADDQRPGSLPPLPGRDP
                     VIDLVRAGALVVVVAWHWAFTTLRWDDGPKVSNPVGVTPGMWLATWFLQVMPLFFLVG
                     GALHAGALDRAHAGGAPTATRPPDLTFVTSRYRRLVVPVLPLLGLAAVAALGAAAIGR
                     TDVVRGIVLMITPLWFLAVYLVLVAVAPIARRLHLRFGLWVPVLGAAVVALVDRALVL
                     PTRGDIGVLPTLAVYVLTWSVVHQLGFHLAALRAAPRMWAWVTAAAGFTALAVGAAVG
                     GYPASMVGTHPDPISNLSPPNLMVVFLAVAQMGSLVVLEPTLRRWCDRHRRLLGTAGA
                     WSMTVYVWHMLALAAFWGLVVVLAGPVDATIDGSWWAQRPLWMVGPLLFAVPLFALTG
                     PRRTPTDRAR"
     gene            2822..4045
                     /locus_tag="PLZ72_12805"
     CDS             2822..4045
                     /locus_tag="PLZ72_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA37223.1"
                     /translation="MGTGRATARRTTARRTGRERVTPTTAALQRRMQLALGALRGVTL
                     VWSAVVTAVDASSGVLDRPAAAAVLIAVLAVWSAMWTAAVAGRQRWVGGAVAVTLDVV
                     LAAAAVGADHWLYAGDHPQSFASAWPLVAVVATAVARGPITGLIAGTVAGLANVVAAL
                     SIGTGMDGEWLSTLGTLVLLAASGWVAGWVADRLRTTAQAAADAQARDEVAATLHDGV
                     LQTLAVIQRRSDDTDLVALAREQDQALRAFLRGGTPTGSAPSAPPRAPSTLPAPSTTS
                     ATTGDGDLIDRLRSEIARIERTHAIDAPLVVIDPGTSTGSAATALIGAAAEAVVNAAK
                     HSGTDRVWVSVDRRSPSGTMVVVHDEGDGFDPATTPEGTGITSSIRRRLDAVGGGATV
                     SSAVGRGTDVTVWAP"
     gene            4042..4683
                     /locus_tag="PLZ72_12810"
     CDS             4042..4683
                     /locus_tag="PLZ72_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA37224.1"
                     /translation="MSTVAGRAVRVVVADDHQIWRAGIRADLGESFAVVGEAEDAPGA
                     IETARRERPDLVLCDLNMPGGGGMAVVQALAAELPVVVLTVSESERDLLDVVAAGAVG
                     YLLKSTRSDDLRASLMRAAEGEPVFSPQLAALLIGEYRRLATTGGDGSLPLSEREREV
                     LAHVARGESYRTVGDALFISPKTVENHVRNAMAKLHLSRRHELIRWAVDHGIT"
     gene            4766..5728
                     /gene="rarD"
                     /locus_tag="PLZ72_12815"
     CDS             4766..5728
                     /gene="rarD"
                     /locus_tag="PLZ72_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNA37225.1"
                     /translation="MRIEADVAGDPARDHRIGVIAGVAAYGLWGFFPIYFHLLAPTSP
                     VEILCHRILWSLAVMAVVLAWRRDGAWIRPLLRRPRQLAELALAALLIAVNWLLYVWA
                     VEQDRVVDAALGYFINPLVTVTLAVVVLGERLRRLQWIAVGLGAVAVAVITVGYGEVP
                     YVALALAVSFAGYGFLKKRVTLAPSQSLAAETAILAPVAAIAMAVMARGNGLEFGSDP
                     AMSALLATSGVVTAVPLVLFAASARRIPLTMLGLLQYLTPTLQFLCGVLIFDEPMSAT
                     RWVGFGLVWAALTFMSVDAVGRLRAGERLQDSDEAVVAATPDLG"
     gene            complement(5774..>7297)
                     /gene="guaA"
                     /locus_tag="PLZ72_12820"
     CDS             complement(5774..>7297)
                     /gene="guaA"
                     /locus_tag="PLZ72_12820"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNA37226.1"
                     /translation="LVVDFGAQYAQLIARRVREARVYSEIVPHTITAAELAARNPAAI
                     IFSGGPKSVRIDDAPVIDPAVYDLGVPILGICYGQQLIAQQLGGEVGGGGAGEYGRTI
                     VHRIDGTDSSLLTDDLPHDQTVWMSHFDAVLRPPDGFVATAGSEGAPCAVIESPERRI
                     WAVQYHPEVAHTPHGQELITRFLHELAGLPGSWTMESFIESSVEAIRAQVGDGRAICG
                     LSGGVDSAVAAALVHRAIGAQLTCVFVDTGLMRKNEGEQVVETFQRHQGIELIHVRAA
                     DRFFERLAGVTEPEDKRKAIGELFIRVFEDARGGVEDARFLVQGTLYPDVIESGTAHA
                     ATIKSHHNVGGLPEDLDFDLVEPLRLLFKDEVRAVGHELGLPDEIVWRQPFPGPGLGV
                     RIIGEVTPEMVALLQEADAIVREELRIAGLEREIWQAFAVLPDIRTVGVMGDERTYGH
                     PVIIRAVTSEDAMTADWARIPYDVLESMASRIINEVPGVNRVAYDITSKPPGTIEWE"
BASE COUNT          889 a         2716 c         2582 g         1110 t
ORIGIN      
        1 gcctgacgct cctgctcggc ggcgatggcg gcgcgcagct cgctgttggc tccgtcgagc
       61 aggtcctgct gagccgacac gctggcttcg aggtcggcct tgatcgagct ctgacgggcc
      121 ttcttgtcct cgagggcctt cgacgcctgc tccaggtcgg cggaccggtc gtcgaggtcc
      181 atctgcgcgg cgcggaggtc atcggcgacc tgctggcgat ccccgtgaag ggtctccatc
      241 aggaccttct cgttgaccgc gacgttgggg tcgcccacac cgaggccggc gacatcggac
      301 ccggcgctca tgtaggcgct cacgatgtag ctgttggcct cggaccgggc ctggtccatg
      361 cgggcctggg cgtcggccac cgcgtcacgg ttcttcttca gctcggtctc ggccttgctc
      421 aactcgatgc cggtctgcag gtactgctcg tcgagctcgg acgcccgggc ctcgaggcgg
      481 tccatctcgg cggccaggcg ctggaccttg gcccgggtgc cctcgacgtc ctgggccgac
      541 gcggtgccgg tcagaccggc gccgacgacc agggccaccg acgcgacgac ggccacggac
      601 cgcgcggcgc gggcggtccg gacgggtcgg gaggagcgcg ggagtgaggt cttcatgacg
      661 gggaccaagt caaccacgtt ccgtggtctg ttacaaacag gtgtcgaacc gacatcgggg
      721 cgagggatgt ctcagccagt ttcatagctt ttcgtgctta cccgtacagc tccactgggt
      781 ggtcccccag ccgaggatgg gggctgaccc cgatgggcgg cggaacccga gggtcccacc
      841 gtggggtcca tgaccgacct cacctcaccc cagaacccga ctgcggccag caccgcagcg
      901 ccgacggcgg gcccggctcc cggcgcaccc accgatccgt cgttcgacgg cggaccccgg
      961 ccccgaccga ccgggccgcc gacccgttgg ggccgacccg atgtgaccga gtggctcgtc
     1021 gcccgacgca acgccggcgc cacccccgcc gtcatcgcca ccgagctggt cgcccgggga
     1081 tgggacgccg acgcggcggc cgacgccgcc cgacggtccc tgcgccgctc cgaccaccac
     1141 cgggtgctgt acggaacgct gtgctggtcg gtcggactcg gcgccctcgg cctggccacc
     1201 ggactgcacc agctcctggc cgacaccccc gaccccgagt gggccgcgct cgccttcacc
     1261 ctggcggtgg tgctgctgcc gctggccgga tggtgcctgt ggtcggctca gcaggtcgag
     1321 gccagctcag cgcacgccgt gtggtccccg acccgacgca tgtggttcgc caccctggcc
     1381 gcctgcaccg cgatcatcgg gctgatccga ctgatcacct acgtctacct ggtgatcgcg
     1441 gcgatgaccg gggcccgctc cacgccgttg acccccgacg accagttcca ggtgctgatc
     1501 tcgttggcca tcgtggtgcc gctgttcgtg tggtcgttcc acgagtggcg ccgatccgac
     1561 gtcgtgctgt cgggcctcaa ggagctggac ggctcggagg gtggacggtg aggcccgccc
     1621 cgaccgtcac cccgggccga gccccggccg tcgccccagc tgtcgccccg gctgtcgccc
     1681 cgggccgagc cgacgaccag cgaccggggt cccttccccc gctgcccggc cgcgacccgg
     1741 tgatcgacct ggtccgcgcc ggagccctgg tggtggtggt cgcctggcac tgggcgttca
     1801 cgacgctgcg ctgggacgac ggaccgaagg tgtccaaccc ggtcggcgtc acgccgggga
     1861 tgtggctggc cacatggttc ctccaggtga tgccgctgtt cttcctggtc ggcggagcgc
     1921 tgcacgccgg cgcgctcgac cgggcccacg ccggcggagc gccgacagcg acccgcccgc
     1981 ccgatctgac attcgtcacg agtcgctacc gccggttggt cgtcccggtg ctcccgctgt
     2041 tgggcctggc tgccgtcgcg gcgctcggcg cggcggccat cgggcggacc gacgtggtcc
     2101 gaggcatcgt gttgatgatc acgccgctgt ggttcctggc cgtgtacctg gtgctggtgg
     2161 ccgtcgcccc gatcgcccga cgactccacc tccggttcgg gctgtgggtg ccggtgctcg
     2221 gcgcggcggt cgtggccctg gtcgaccggg cgctcgtgct gcccacccgc ggagacatcg
     2281 gcgttctgcc cacgctggcg gtctacgtcc tgacgtggtc ggtggtgcac cagctcgggt
     2341 tccacctggc cgcgctgcgc gccgcacccc gcatgtgggc gtgggtgacc gccgcggcag
     2401 gcttcaccgc cctcgccgtc ggggcagccg tcgggggcta cccggcgtcg atggtcggca
     2461 cccaccccga cccgatctcg aacctgtcgc caccgaacct gatggtcgtg ttcctggccg
     2521 tcgcgcagat gggttcactg gtggtcctcg agccgacgct gcgacggtgg tgcgaccgac
     2581 accggcgtct gctcggcacc gccggggcat ggtcgatgac cgtgtacgtg tggcacatgc
     2641 tcgccctcgc ggcgttctgg ggactggtcg tcgtgctcgc cggtccggtc gacgcgacca
     2701 tcgacgggtc gtggtgggcg caacgtccgc tgtggatggt gggcccgctg ctgttcgccg
     2761 tgccgctgtt cgctctgacc ggaccgcgcc ggacgccgac cgatcgggca cgatgacctg
     2821 gatgggaacc ggccgcgcca ccgcacgccg caccactgct cgccgcaccg gacgggaacg
     2881 ggtgacgccg accaccgcgg cgctgcagcg acggatgcag ctggcgctcg gcgcgctgcg
     2941 aggcgtcacg ctggtgtggt cggcagtggt gaccgcggtc gacgcatcct ccggggtgct
     3001 cgaccgtccc gcggctgccg cggtgctgat cgccgtcctc gcggtgtggt cggcgatgtg
     3061 gaccgcggcg gtcgccggtc gacagcggtg ggtgggcggt gcggtggcgg tcacactgga
     3121 cgtcgtgttg gccgcggccg ccgtcggggc cgaccactgg ctctacgccg gcgaccaccc
     3181 gcagtcgttc gcgtcggcgt ggccgctcgt ggccgtcgtc gccaccgcgg tggcccgcgg
     3241 gccgatcacc ggcctgatcg ccggcacggt cgccgggctc gccaacgtcg tggccgcgct
     3301 cagcatcggg accggcatgg acggcgagtg gctgtccacc ctcggcaccc tcgtcctgct
     3361 cgccgcgtcc gggtgggtgg cgggctgggt ggccgaccgg ctccgcacca ccgcacaggc
     3421 cgcggccgac gcccaggccc gcgacgaggt cgccgccacc ctccacgacg gcgtgctgca
     3481 gacgctggcg gtgatccaac gacgttccga cgacaccgac ctggtcgccc tggcccggga
     3541 gcaggaccag gcgttgcggg ccttcctccg cggcggcact ccgaccgggt cggccccctc
     3601 ggcgcccccg cgggccccgt cgaccctgcc cgccccatcg accacatcgg cgaccaccgg
     3661 cgacggtgac ctgatcgacc gcctccgcag tgagatcgcc cgcatcgagc gcacccacgc
     3721 gatcgacgct ccgctcgtgg tcatcgaccc cggcacctcg accggatcgg ccgccaccgc
     3781 cctgatcggc gcggcggccg aggcggtggt caacgcggcc aagcactccg ggaccgaccg
     3841 cgtgtgggtc agcgtggatc gccgcagccc gtcgggcacg atggtggtcg tgcacgacga
     3901 aggcgacggg ttcgaccccg ccaccacccc cgagggaacg gggatcacct cgtcgatccg
     3961 ccgacggctc gacgcggtgg gcggcggggc gaccgtgtcc agcgcggtcg gacgcggcac
     4021 cgacgtgacg gtctgggccc cgtgagcacc gtcgcgggtc gggccgtgcg ggtcgtggtc
     4081 gcggatgacc accagatctg gcgggcgggc atccgggccg acctcggcga gagcttcgcg
     4141 gtggtcggcg aggccgagga cgcccccggc gcgatcgaga ccgcccgccg cgaacggccc
     4201 gacctggtgc tgtgcgacct gaacatgccc ggcggcggcg ggatggcggt cgtgcaggcg
     4261 ctcgccgccg agctccccgt cgtcgtcctc accgtcagcg agtccgagcg ggacctgctc
     4321 gacgtcgtcg ccgccggagc ggtcggctac ctgctcaagt cgacccgctc cgacgatctg
     4381 cgcgcctcgc tcatgcgagc cgcggagggc gagccggtgt tctcaccgca gctcgccgcg
     4441 ctgctcatcg gcgagtaccg ccgcctggcc accaccggcg gcgacgggtc gctgccgctg
     4501 agcgaacgcg agcgcgaggt gctcgcgcac gtggcccgag gcgagtcgta ccggaccgtc
     4561 ggcgacgcgc tgttcatctc gcccaagacc gtcgagaacc acgtgcgcaa cgcgatggcc
     4621 aagctgcacc tgtcgcgacg ccacgagctg atccgctggg ccgtcgacca cggcatcacg
     4681 tgaccacccc cgtctcaccc accccggcct cacccacccc gctgcgttct catgaccgtt
     4741 cgcgccacca gtggcacgag gagtcatgag aatcgaggcc gacgtggccg gtgaccccgc
     4801 ccgcgaccat cggatcggcg tcatcgccgg ggtggccgcc tacgggctct ggggattctt
     4861 cccgatctac ttccacctgc tggcgccgac ctcgcccgtc gagatcctct gccaccggat
     4921 cctgtggtcg ctcgcggtca tggccgtcgt gctggcctgg cgacgcgacg gcgcctggat
     4981 ccgaccgctc ctgcgccgac cgcgccagct cgccgagctg gcgttggccg cgctgctgat
     5041 cgccgtcaac tggctgctgt acgtgtgggc ggtcgaacag gaccgggtgg tcgacgccgc
     5101 gctcggctac ttcatcaacc cgctggtcac cgtgacgctc gccgtcgtcg tgctgggcga
     5161 gcggctccga cggctccagt ggatcgcggt cggcctgggt gcggtcgcgg tcgcggtgat
     5221 caccgtcggc tacggcgagg tgccctacgt cgcgctcgcg ctcgccgtgt cgttcgccgg
     5281 ctacggcttc ctcaagaagc gggtgacgct cgctccgtcc cagtcgctgg cggcggagac
     5341 cgcgatcctg gccccggtcg cggcgatcgc catggcggtc atggcccgcg gcaacggcct
     5401 ggagttcggc agcgacccgg ccatgtcggc gctgctcgcc acgtccgggg tggtcaccgc
     5461 ggtcccgctg gtcctcttcg ccgcgtcggc ccgacgcatc ccgctcacca tgctcggcct
     5521 gctgcagtac ctgacgccga cgctgcagtt cctgtgcggc gtgctcatct tcgacgaacc
     5581 gatgtcggcc acgcgttggg tcgggttcgg tctcgtctgg gcggcgctga cgttcatgtc
     5641 ggtcgacgcc gtgggtcggc tgcgggccgg ggagcggttg caggactccg acgaggcggt
     5701 cgtggccgca acccccgacc tgggctgagg tccgggagcc cgcccacgcc aaccggtcag
     5761 cgccggtcgg cggtcactcc cactcgatcg tgcccggcgg cttcgacgtg atgtcgtagg
     5821 ccacccggtt cacgcccggc acctcgttga tgatccggct cgccatcgac tccagcacgt
     5881 cgtaggggat gcgggcccag tccgcggtca tcgcgtcctc cgacgtcacc gcccggatga
     5941 tcaccggatg cccgtaggtg cgctcgtcgc ccatcacccc gacggtgcgg atgtcgggca
     6001 gcaccgcgaa cgcctgccag atctcgcgct ccaacccggc gatacgcagc tcctcgcgga
     6061 cgatggcgtc ggcctcctgc agcagcgcca ccatctcggg ggtcacctcg ccgatgatgc
     6121 gcacccccag acccggaccc gggaacggct gacgccacac gatctcgtcg ggcaggccga
     6181 gctcgtggcc caccgcgcgc acctcgtcct tgaacagcag ccgcagcggc tcgaccaggt
     6241 cgaagtccag gtcctcggga aggccgccga cgttgtggtg gctcttgatg gtggcggcgt
     6301 gcgcggtgcc cgactcgatc acgtcggggt acagggtccc ctgcaccagg aaccgtgcgt
     6361 cctccacgcc gccgcgggcg tcctcgaaca cccggatgaa cagctcgccg atcgccttgc
     6421 gcttgtcctc gggttcggtc accccggcca ggcgctcgaa gaaccggtcc gcggcccgga
     6481 cgtggatgag ctcgatgccc tggtggcgct ggaacgtctc caccacctgc tcgccctcgt
     6541 tcttgcgcat cagcccggtg tcgacgaaca cgcaggtgag ctgcgcgccg atcgcccggt
     6601 gcaccagcgc ggcggccacc gccgagtcga ccccgcccga cagaccgcag atggcccgac
     6661 cgtcgccgac ctgggcccgg atcgcctcga ccgacgactc gatgaagctc tccatggtcc
     6721 acgagccggg caggccggcc agctcgtgga ggaaccgggt gatcagctcc tggccgtgcg
     6781 gggtgtgggc gacctcgggg tggtactgca ccgcccagat ccgccgctcg ggcgactcga
     6841 tcaccgcgca cggcgcgccc tcggaacccg cggtggcgac gaagccgtcg ggcggacgca
     6901 gcaccgcgtc gaagtggctc atccacaccg tctggtcgtg gggcaggtcg tcggtgagca
     6961 gcgacgagtc ggtgccgtcg atgcggtgca cgatcgtgcg gccgtactcg ccggcgccgc
     7021 cgccgccgac ctcgccgccc agctgctggg cgatgagctg ctggccgtag cagatgccca
     7081 ggatcggcac gcccaggtcg tagacggccg ggtcgatcac gggggcgtcg tcgatgcgca
     7141 ccgacttggg cccacccgag aagatgatcg ccgcggggtt ccgcgccgcg agctcggctg
     7201 cggtgatcgt gtgcgggacg atctcggagt acacccgggc ctcgcggact cggcgggcga
     7261 tcagctgcgc gtactgggcg ccgaagtcga ccaccag
//
LOCUS       DAOQOD010000154         7282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3875_length_7282_cov_10.433928, whole genome shotgun sequence.
ACCESSION   DAOQOD010000154 DAOQOD010000000
VERSION     DAOQOD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_7282_cov_10.433928"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..999)
                     /locus_tag="PLZ72_12825"
     CDS             complement(193..999)
                     /locus_tag="PLZ72_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37227.1"
                     /translation="MAMKAGMRRAALVAGLVLVAVTAASCGESSNASAPKSTTADGAS
                     SSTAQDPFCDKWVEVDGEGQKLSDPSVPPDVRTQAAGAVVAALDAAPAPDAITTDVST
                     IKAGIQPITNGEPGDTESPEFTSAQLAVGRWVHSECAFQTVELSTEEYHYSNLPDTLE
                     SGVTSFAIENHGNEPHMMLLAKVDPKVNKSLDELIQESSTSDGTPEGVTEMGGGGIAM
                     PGSTGYFTVDLEPGRYAIVCPLPVGWNGQGPPPEAPPHMTQGMAAEFTVE"
     gene            1238..1969
                     /locus_tag="PLZ72_12830"
     CDS             1238..1969
                     /locus_tag="PLZ72_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37228.1"
                     /translation="MRRVVRTFEGDRAPARALRDASLTVREGEFVAVVGPSGCGKSTL
                     LNLVAGLDVPDEGSVTVLGHRMDELSLDERAVLRRESIGMVFQFFHLLDSMTVLDNVA
                     LPRRLGGATRVAAERRGMDLLDLLGLADLATSTIGTLSGGQRQRLAIARSLANEPALL
                     LADEPTGALDSEGGDEVMELFTRLHQSGRTIVMVTHEPLVAGRADRVVDLADGRTVTV
                     SDVVASDVVASDVGGAVVGPTGIDR"
     gene            2053..4368
                     /locus_tag="PLZ72_12835"
     CDS             2053..4368
                     /locus_tag="PLZ72_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNA37229.1"
                     /translation="MRWVKVVGLAVLVAIGTGAVGATLAGARRSAGVLERLNAITRPA
                     DAYALANIEGFDWEEVRRLPNVSQLVTFAVVYFHVEQLDRMVGGFPPVGDTSQLSRIE
                     RPVLVAGRLPDQSRPDEVTIAPEVHAEGVEIGDVLTLDLRPIDDSGTEISDSSRARVK
                     VVGIAKNAFFAWDVQPTAAFWAEHSDGGLTPGYINALVRLRTGEAGIESFARDLQRIA
                     GHPVEVNGVAAGNRTNSRTIDIETAALLVLTIAMATVSTVLLCQALLRLIRPSEPGLG
                     VVALLGTTHRTLWTMAMTVPAVTLIVGVLVSPLVALALSPLFPIGNGRKFEPTPGMRT
                     DWVAWGTGAGWIALACGSAVAVAAWRTTRWISTAPAHPLQRPVRPPSIPRRRLPWGSS
                     VPFEQGVRLALGPGGGRGVAWSVGSGVAVIVAAVTFAGGIDRAVTDSTLFGQHYAVGT
                     SLDQDPALTASEVVKETPASRAGHLRNTVIEIDGVPTSTVSVESLQGSSPLRALSGRL
                     PRRDDEIALSPDAAELLDAGVGDTVPITGPTAPGRTGDEGSRSVPRPRLRVVGVMFVP
                     HTSHTDYTSGAVVTPGLTRRLVEAGAPLKLDAVVWSPTRGTSVGEALASLDRRIAAAT
                     DASIPVDRQSALSGTRPLPRWLAAVAAALTLGATTHVLAASARRRRSEVAVMQVLGLT
                     RRQARSITLWHASIGAVVGTALAIPVGFAVGRTLWQATAALLPFRYATPHSWTAVTIA
                     SVSVIVLTAIVALISARRSGQAEPSLVLRTE"
     gene            4376..5845
                     /locus_tag="PLZ72_12840"
     CDS             4376..5845
                     /locus_tag="PLZ72_12840"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNA37230.1"
                     /translation="MRQLWVASSAFVVGVVGAALTLLAMLGAPTVDERGVIAAAVVSG
                     LVGLASGMATRQLVSSWWPPESKVPKDVTERLSDPSTRTLPLDEVIQQVVDSLRSIEP
                     RRRVEVWQARGGRLHLTHSLPPTDAPPIDVGRQSASTAAVSGVVGQGWLRTWLPSLLD
                     AGSAIAVRPGAGGAPDADGGPPDLVRAAPMAARGELLGMVVLRRAAQDPPFSAAEAER
                     LAQVQRPLAGILNESRLAGQLRASVDELQARNTELQRSRQRLVAVADAERRRLERDLH
                     DGAQARLTALGVRVQVARTLATRSPERVGELLDEVAGELAEAVGELRSLAHGIMPPLL
                     LSGGLRDALPEAAGRSRVPTTVEPISPERFGHEVEAAVYFCCVEALQNVAKHAGTGAR
                     AHVAVHRVGEELHFEVDDDGSGFTTSRRAGAPTDGGDGEPRPDGGRRGVDVPGRGQGL
                     TNIVDRVGALGGTVAFGTSDRGGAVVSGVIPLPARALGS"
     gene            complement(5870..6538)
                     /locus_tag="PLZ72_12845"
     CDS             complement(5870..6538)
                     /locus_tag="PLZ72_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA37231.1"
                     /translation="MRVVIAEDNYLLRQGLQQLLELDGRVEVVATCADAEDLLRSVDD
                     LRPDVVLTDIRMPPTHTDEGIRAAIEIRRRHPDIGVVVLSQYASPAHAVALLGDESTG
                     LAYLLKDRVGDVAHLVSAIETVRGGGSVVDSEVVRALISARSVTSNSPLAVLTSRETE
                     VLAAMAEGLSNAAIAASTHAAERTVEKYISSIFSKLGLADEPDANRRVRAVLVYLAAS
                     DQTA"
     gene            complement(6649..7065)
                     /locus_tag="PLZ72_12850"
     CDS             complement(6649..7065)
                     /locus_tag="PLZ72_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA37232.1"
                     /translation="MEQRLSLVTLGVDDIERSRTFYEAVGWSGESPDGEVWFFQCGGI
                     VVALWSRRLLAADSGVVDGGAWGGVTLAHNVTSPAEVDAVIAAAEDAGATIARPGAPT
                     DWGGYSGVFVDPDGHPWEVAHNPHWTVTPDGSTLMH"
BASE COUNT         1023 a         2541 c         2600 g         1118 t
ORIGIN      
        1 accactggta cgaccccgcg cacgacgccc atcccgagct cggcatccgc ccgctggact
       61 gggacgctga cgggtggccg gtcgcacgtg ggtggtcccc cgtcgtcgcc ctccccgcac
      121 cgccgggcgt ccacaactga gatccgggca cgatggcgac aaccgtcgcc atcgcgccct
      181 cggaacctcg gctcactcca cggtgaactc tgcggccatc ccctgggtca tgtggggcgg
      241 agcctcgggg ggcggcccct gaccgttcca gccgaccggc agcgggcaca cgatcgcgta
      301 ccgaccgggc tcgaggtcga cggtgaagta gccggtcgac ccgggcatgg cgatgcctcc
      361 cccgcccatc tccgtgacgc cctcgggcgt tccgtcggac gtcgaggact cctggatgag
      421 ctcatcgagc gacttgttca ccttcgggtc caccttggcg agcaacatca tgtgcggctc
      481 gttcccgtgg ttctcgatgg cgaaggaggt gacgccggac tcgagggtgt ccggcaggtt
      541 gctgtagtgg tactcctcgg tgctcaactc gaccgtctgg aaggcgcact ccgagtgcac
      601 ccaccggccc acggcgagtt gggccgaggt gaactcaggg ctctcggtgt cgccgggttc
      661 gccgttcgtg atcggttgga tgcccgcctt gatcgtcgac acgtccgtgg tgatcgcgtc
      721 cggcgccgga gcggcgtcca aggcggccac cacggcaccc gcggcctgcg tgcggacgtc
      781 gggcggaacc gaagggtcgc tgagcttctg accctcgccg tcgacctcta cccacttgtc
      841 acagaacgga tcctgcgccg tcgatgagga cgcaccgtcg gccgtcgtgg atttgggtgc
      901 cgaggcgttg ctgctctcac cgcagctcgc cgcggtcacc gcgaccagga cgaggccggc
      961 gaccagcgcg gcccgtcgca tccctgcctt cattgccatg tcgactcctt cgtgctcgga
     1021 tccttcgacg gtacgaagca ccactcgtcg ccgataccgt gccgaccgcc cgatccgggg
     1081 cggcaaaccc tcccgacgga tacgggctgc gctctccacc gacacgacga tccctgctcg
     1141 aatggactcg atgacgagca cgtcggcgcc cccgagcggt gaaggccggt cgtgctcgaa
     1201 cgcgtccgat cccgtcggag ccacgaccgt cgtcgacgtg cgacgggtcg ttcgcacctt
     1261 cgagggagat cgtgcgccag cacgggcact gcgcgacgcg agcctgacgg tgagagaggg
     1321 agagttcgtc gctgtggtcg gaccatccgg ctgcggcaag tccaccctgc tcaacctcgt
     1381 cgccggcctc gacgtgcccg acgaggggtc cgtcacggtg ctcggtcacc ggatggacga
     1441 gctgtcgctg gatgaacggg cggtgttgcg gcgggagtcg atcggcatgg tcttccagtt
     1501 cttccacctc ctcgactcga tgacggtgct ggacaacgtc gcactccccc gacggctggg
     1561 aggagcgacc cgtgtcgccg ccgagcgccg ggggatggat ctgctcgacc tgctcggctt
     1621 ggcggacctc gccacctcga ccatcggcac gctctccggt gggcagcgac agcggttggc
     1681 gatcgcccgt tccctcgcga acgaaccagc gctgctcctc gccgacgagc ccacgggagc
     1741 cctcgacagc gagggtggtg acgaggtgat ggagctgttc acccgcctgc atcagagcgg
     1801 ccgaacgatc gtgatggtga cccatgagcc cctggtggcg ggccgcgccg atcgcgtggt
     1861 cgacctggcc gatggccgaa ccgtgaccgt gagcgatgtg gtggccagcg atgtggtggc
     1921 gagcgacgtc gggggggccg tcgtcggccc gaccgggatc gatcggtgag ccgaccgggg
     1981 ccggagcgcg agccgacatc gcggtcgatc gcggtctgct cctggcggac gtggtcggcg
     2041 cacgaccttt cgatgcgctg ggtcaaggtc gtcggcctcg ccgtcctcgt cgccatcggg
     2101 accggggcgg tcggagccac gttggccggg gccaggcgca gcgctggcgt tctcgaacgc
     2161 ctcaacgcca tcacccggcc cgccgacgcg tacgccctcg ccaacatcga gggcttcgac
     2221 tgggaggagg tccggcgtct tcccaacgtg agccagctcg tgaccttcgc cgtcgtgtac
     2281 ttccatgtcg agcaactcga tcggatggtc ggcggcttcc caccggtcgg cgacacctcc
     2341 cagttgtcgc ggatcgaacg gccggtgctc gttgccggac gcctgcccga ccagtcccgt
     2401 ccggacgagg tgacgatcgc tcccgaggtc catgccgagg gtgtcgagat cggagacgtg
     2461 ctcacactcg acctgcgccc gatcgacgac tccggcacgg agatctcgga ctcgtcacgg
     2521 gcacgcgtga aggtggtcgg tatcgccaag aacgcgttct tcgcatggga cgtgcagccg
     2581 accgcggcgt tctgggccga gcactccgac ggcggcctga cacccggcta catcaacgcg
     2641 ctggtccgcc tgcggaccgg cgaggcgggc atcgagagct tcgcccggga cctccaacgg
     2701 atcgcgggtc accccgtcga ggtgaacggg gtggcggccg ggaaccggac gaacagccgg
     2761 accatcgaca tcgagaccgc ggcgttgctg gtgctgacca tcgcgatggc gacggtgtcg
     2821 acggtgctgc tgtgccaggc actcctccgt ctgatccgcc cgtcggagcc gggtctgggc
     2881 gtcgtcgcgc tgctcggcac gacgcaccgc acgttgtgga cgatggcgat gacggtcccg
     2941 gcggtgacgc tcatcgtcgg ggtcctcgtc tcgccgctgg tggcgctggc gctctctccg
     3001 ctgttcccga tcggcaacgg ccgaaagttc gagccgacgc ccgggatgcg caccgactgg
     3061 gtcgcctggg gtaccggcgc cggctggatc gccctcgcat gcgggagcgc ggtcgccgtc
     3121 gccgcgtggc gaacgacccg gtggatcagc acggcgcccg cccatccgct ccagcgtccg
     3181 gtccgcccac cttcgatccc gcggcggagg ctgccctggg gatcgtcggt gccgttcgaa
     3241 cagggagtgc gattggccct gggccccggc ggtggccgtg gagtcgcctg gtcggtgggc
     3301 tcgggtgtgg cggtcatcgt ggcggcggtg acgttcgcag gcggcatcga tcgggcggtg
     3361 accgactcca ctctgttcgg ccagcactac gccgtgggga cctcgctgga ccaggaccct
     3421 gcgctgacgg catcagaggt ggtgaaggaa actcccgcgt cgagggccgg acacctgcgc
     3481 aacacggtca tcgagatcga cggcgtcccg acgtcgacgg tcagcgtgga gagcttgcag
     3541 gggagctcac cgctccgggc gctcagcggg aggctcccgc gacgcgatga cgagatcgcc
     3601 ctgtcaccgg atgcggccga gttgctcgac gcaggggtcg gtgacaccgt tccgatcacg
     3661 ggtcccacag caccgggccg gaccggggac gagggttcac ggagcgtccc gcggccccgg
     3721 ctccgtgtcg tcggagtgat gttcgtccca cacacctcgc acaccgacta cacgtccgga
     3781 gcggtggtca cgccggggct gacccgacgg ttggtcgagg ccggggcgcc gctcaagctc
     3841 gatgccgtgg tctggtcgcc gacgcgcggg acgtcggtcg gggaggcctt ggcctcgttg
     3901 gatcgacgga tcgccgcagc caccgatgcg tccattccgg tggaccgcca atcggcgctg
     3961 tcaggaacgc gaccgctccc gaggtggctc gccgcggtcg cggcggcgct cacgctcgga
     4021 gcaacgaccc acgtcctcgc ggcgtccgcc cgccggagga ggagcgaggt ggcggtgatg
     4081 caggtgctcg gcctgacccg gcggcaggcc cgatcgatca cgctgtggca cgcgtcgatc
     4141 ggcgcggtcg tcggcaccgc cctggcgatc cccgtcggct tcgccgtcgg ccggacgttg
     4201 tggcaggcga cggcggccct gttgccgttc cggtacgcga caccgcactc atggacggcc
     4261 gtcacgatcg cgtcggtgtc ggtgatcgtg ctcacggcga tcgtggcgct gatatccgca
     4321 cgcagatccg gccaggccga gccctctctc gtcctgcgga cggaatgagc gacccgtgag
     4381 gcagttgtgg gtcgcgtcga gcgcgttcgt cgtcggcgtc gtcggcgccg cactgacgct
     4441 gctggcgatg ctcggcgcac cgacggtgga cgagaggggt gtgatcgccg cagctgtcgt
     4501 gtcgggtctc gtcggtctcg catccggaat ggccacccgg caactggtga gttcgtggtg
     4561 gcctccggaa tcgaaggttc cgaaggatgt gacggaacga ctgtcggacc cctccacacg
     4621 cacgttgccc ctcgacgagg tgatccaaca ggtggtggac tcgctccgca gcatcgagcc
     4681 ccggcgacgc gtcgaggtgt ggcaggcacg gggcggtcgc ctccatctca cccactcgct
     4741 gccgccgacc gatgccccac ccatcgacgt gggccggcag tccgcgtcca ccgccgcggt
     4801 ctcgggggtg gttggtcagg ggtggctgag gacctggctg ccgagcctgt tggacgctgg
     4861 gtcagccatc gcggtccgcc ccggcgctgg gggtgctccc gacgccgacg ggggccctcc
     4921 cgatctggtc cgggcggcgc cgatggcagc gcgcggcgaa ctgctcggaa tggtggtgct
     4981 gcgtcgggcc gcccaggatc cgccgttctc ggcggccgag gccgagcgcc tcgctcaggt
     5041 gcaacgcccg ctggccggca tcctcaacga atcccgcctg gccggacagc tccgagcctc
     5101 cgtcgacgag ctgcaagcac gcaacaccga gctccaacgg tcacgacagc gactggtcgc
     5161 cgtcgccgac gcggaacggc gtcgtctcga gcgggacctg cacgacggcg cccaggcgcg
     5221 cctgaccgcg ctcggagttc gggtgcaggt cgctcgaacc ctggcgacgc gatcgcccga
     5281 gcgcgtcggt gagctcctcg acgaggtggc gggcgagctc gccgaggcgg tcggcgagct
     5341 gcgcagcctc gctcatggga tcatgccgcc gctgctgctg tcggggggtc tgcgcgatgc
     5401 gctacccgag gcggccggca ggagccgggt ccccacgacg gtcgagccga tctcccccga
     5461 gcggttcggc cacgaggtcg aagcggccgt ctacttctgc tgcgtcgagg ccctgcagaa
     5521 cgtggcgaag cacgcgggta ccggcgcacg cgcccacgtg gcggtgcacc gagtcggcga
     5581 ggagctgcac ttcgaggtgg acgacgacgg ttccggcttc accacgtccc gtcgagcggg
     5641 agccccgacc gacggcgggg acggcgagcc gcgtcccgac gggggacgcc gcggcgtcga
     5701 tgtcccgggg cgaggtcagg gcctcaccaa catcgtcgac cgcgtcggcg ccctgggggg
     5761 aacggtcgcc ttcgggacat cggatcgggg cggcgcggtg gtgtcggggg tgatcccgct
     5821 gccggcacgt gcgctcgggt cctgatccgg ctgcacccgc aggatcgagt cacgcggtct
     5881 ggtccgacgc ggcgaggtac acgaggacgg cacggacccg gcggttggcg tcgggctcgt
     5941 ccgcgagacc cagcttcgag aagatcgagc tgatgtactt ctccaccgtg cgctccgcgg
     6001 catgggtcga cgcggcgatc gccgcgttcg acaagccctc ggccatcgcc gcgagcacct
     6061 ccgtctctcg ggacgtcagc accgccaacg ggctgttcga ggtgaccgat cgcgccgaga
     6121 tcagcgctcg cacgacctcg ctgtcaacca ccgaaccacc gcccctcacc gtctcgatgg
     6181 ccgacacgag gtgggcgaca tcaccgacac ggtccttcag gaggtaggcg aggccggtcg
     6241 actcgtcacc cagcagggcg accgcatgcg ccggactcgc gtactggctg aggaccacga
     6301 caccgatgtc ggggtgtcgt cgacggatct cgatcgccgc acggatcccc tcgtcggtgt
     6361 gggtcggtgg catccggatg tcggtcagca cgacgtcggg tcggaggtcg tcgaccgacc
     6421 ggagaaggtc ctccgcgtcg gcgcacgtcg cgaccacctc gacgcgcccg tccagctcga
     6481 gcagttgctg cagcccctgt cggagcaggt agttgtcctc cgcgatgacc acgcgcaccc
     6541 acccatcgtg gcccggaagc cgagcgacgt cgcaagtcca ccgcctccgg cccgccaccg
     6601 gacaccgacc cggcccctgc gcacgggcgg gaccgtccgt ccagctcgtc agtgcatcag
     6661 ggtcgagccg tcgggggtga cggtccagtg ggggttgtgc gcgacctccc acgggtgccc
     6721 gtccgggtcg acgaacacac ccgagtagcc accccagtcg gtcggcgcgc cgggccgggc
     6781 gatcgtggcg ccggcatcct cagcagcagc gatgactgcg tcgacctccg ccggcgaagt
     6841 gacgttgtgg gccagggtga cgcctcccca agcgccgccg tcgacgacac cgctgtcggc
     6901 cgccagcagt cgccgcgacc agagggctac gacgatgccg ccgcactgga agaaccagac
     6961 ctcgccgtcg ggcgattccc cgctccagcc gacggcttcg tagaacgtgc gggacctctc
     7021 gatgtcatcg acaccgaggg tcacgaggga cagtcgttgt tccatgtcgc ctcccgggag
     7081 gggccgctga gcctattccg ctcgaaccag cgttcgcgtg ggcgagtctc tcgtcgccgg
     7141 gcgtcggctg agcggagcgg gacgggcatc aggggtcgag cagcgcgatg gcgctcgact
     7201 cgaccggttc cgtaggttgg ctcacgttga cgcgggagcg gtccgcgtcg gtcacggggg
     7261 aacgacattg acatcgacga cg
//
LOCUS       DAOQOD010000155         7280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3877_length_7280_cov_11.644291, whole genome shotgun sequence.
ACCESSION   DAOQOD010000155 DAOQOD010000000
VERSION     DAOQOD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_7280_cov_11.644291"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..871
                     /locus_tag="PLZ72_12855"
     CDS             119..871
                     /locus_tag="PLZ72_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37233.1"
                     /translation="MLTETWVIYDRQIRSNIRNPVWVFFGLTQPILYLVLFGPLLTNV
                     SGGGLGGDESWLIFVPGLLLQLTIFGAGFAGFGIIQELREGVVERQRVTPAHRSALIL
                     GRSLSNTVTIGVQAVILVLVAIPFGLRPSWWGVVVSVVMVSVLSLGLAAASYAMGLIL
                     KDEDSFAPFVQGVSLPLLLLSGVFLPMTLAPAWLEKMSQANPLTYVLDGTRSLFAGDW
                     GARAISVGIVCTVVVTVALTWWSARRFQRLSS"
     gene            complement(962..1990)
                     /locus_tag="PLZ72_12860"
     CDS             complement(962..1990)
                     /locus_tag="PLZ72_12860"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNA37234.1"
                     /translation="MTEVVDPVGALARQLARHPREPLADSPTPLRAAPRLSEGLGQEV
                     WVKRDDLTGFGLGGNKVRKLEFLLGRARADGCDTIVTFGALQSNHARQTAAACAADGL
                     RCELILTRAVARSGEAFEHGGNLALDHLFGATVTVVDDDDDAIAAAMGTLHEQIAARG
                     SVARWVPPGGSDAVGTLGYVAAGVELAEQITASGLTVDDVVVAASTGGTYAGLLTGLR
                     LSGIGSRLHGICVYRDAEHTRDAVEGLLVGVADLLGTPPVPFDEVLLDDAHLGAGYGL
                     PSPGTLDAMRLFARTEALVLDPVYTGKAAEALVARARAGTLGPGVTVFVHTGGAPALG
                     AYPDLDLA"
     gene            2152..2388
                     /locus_tag="PLZ72_12865"
     CDS             2152..2388
                     /locus_tag="PLZ72_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HNA37235.1"
                     /translation="MDVRIGVTHSPREIDLQLADDVDRDELRSRIDEVLADDSRVLWL
                     TDRKGAEVGIPSARISYVEIGSSDEGRSFGFSST"
     gene            2453..3478
                     /locus_tag="PLZ72_12870"
     CDS             2453..3478
                     /locus_tag="PLZ72_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNA37236.1"
                     /translation="MSAGPVGLLDRKLLFVTGKGGVGKTSVASAIAMLAASRGKRTLV
                     CEVDAKGNLADFLGVGPLRFQPTEVRKRLFAMSMDTEESLKEYMRLNLKLPLLAKMGP
                     LARTFEFVANAAPGVNELLTVGKFTWEVRERNYDLVVVDASATGHVVGQLAAPVAIND
                     LVKVGLVRSQTDWMIDILTDADTTGVVVVTVPEEMPVLETLELIERLRGETEIDVASV
                     VVNRVLPELFGRAEEEVFERLRKPDGVAALSARVGRVAAGTGAPDDVADHDVVHDDSA
                     GVDEVLDGAELAVSLRRSRVPHLDRLRDGLPAGLPVLYLPYLFHRSHGVRATARIAEG
                     LGEELGF"
     gene            3514..>4673
                     /locus_tag="PLZ72_12875"
     CDS             3514..>4673
                     /locus_tag="PLZ72_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNA37237.1"
                     /translation="MAPATIEQLCSAKEVVVSTGSGGVGKTTTAAAIGASAATHLGGK
                     VLVLTVDPAKRLANALGLERFGNVETQVPPEAFTSAGVQPEGELWAAMLDTKQSWDDL
                     VRLHAPDAATRDAILVNPLYQNITGKFVQSHDYVAMERLYEIHTSGRYDLIVVDTPPT
                     RNAVDFLDAPDRMADFFSSRLLRWLITPYRSRLVSFASKPFYSVADRILGTQFLEDIA
                     EFFMLFQTMYDGFVERARAVTTLLGGPQTTFVVVSTLEVAPAREAEYFLKLLGERGYH
                     VGALVLNKVLPELFLDRDATTVAKTLAHHSAEVAADVGARIGPPADDERLLTTVLGEV
                     GESFLNYQVVARREAEQRAELARIPQLTATVPYFDDDIHSLGGLLRLGERIW"
     assembly_gap    4674..4773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4774..6321
                     /locus_tag="PLZ72_12880"
     CDS             <4774..6321
                     /locus_tag="PLZ72_12880"
                     /inference="COORDINATES: protein motif:HMM:NF023702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNA37238.1"
                     /translation="DDETSFTAEHVALVVPPVDPEASRHLKRLVAGWGMLADFAFADL
                     LLYVALPSPDAGPERYLVVNQVRPNTGQTLFNDDVVGRTMSPAQRPLVARARAESEIC
                     EDVIDSPWLGERIRVTAIPVRFEGRVVAVLAREASLEGARDASDLASVYLGVFRRLGR
                     MVADGVFPFVEEEVIATGGPRVGDGVLLLDEMGRVAFASPNAVSALRRLGVQRRLIGE
                     HLSELGAETAATYRAYFNGRPTVEEVERDDVSVVLRCIPLITDGRVDGAVVLMRDVSE
                     MRSRDRALVSKDATIKEIHHRVKNNLQTISSLLRLQGRRLHEPSAKAAIEESVRRIRS
                     IALVHETLSREDGDDVDFGEVVRSLVRMVEEGLTSPDTPVELVIEGGAGKLPSPEATS
                     LAVVITELLQNVVDHAFRPGTLNPGERGRIRIVMAHDQVVLRLAVIDDGAGFPDDFDP
                     ETSDSLGLSIVRGLIDELGGTMEFTAVRPGERRPGNRVQLTLPVVSRVEADAQRPPPG
                     GGRSVGL"
     gene            complement(6338..6637)
                     /locus_tag="PLZ72_12885"
     CDS             complement(6338..6637)
                     /locus_tag="PLZ72_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA37239.1"
                     /translation="MALTSSRSLNLATDDWRAASACSDTDPSLFFPVGTTGPALEQIA
                     AAKAVCDTCEAKEPCLQFALSTNQDSGVWGGTSEEERRKLRRAWVAAQRKQAQAS"
     gene            6883..>7280
                     /locus_tag="PLZ72_12890"
     CDS             6883..>7280
                     /locus_tag="PLZ72_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNA37240.1"
                     /translation="MSTTHRPLVIAHRGASADEPENTASAFRAAAEQGADWVELDVRL
                     AADGSLIVNHDAWYRDERTVWSTPFDQVPPDTLDLAAALAACGDMGVNIEIKNSPGDL
                     ADGPWSLEVVDVTVELIGTLDAAARDRILVS"
BASE COUNT         1019 a         2530 c         2534 g         1097 t
ORIGIN      
        1 gccacctcgc gatgccggtc ctcggactcg ccggccatcc cgccgacccc ggccatcccg
       61 ccgaccccag ccatcccgct catcccgctc atcccgcccg cactgaagga ggcctcccgt
      121 gctgaccgag acctgggtca tctacgaccg acagatacgc agcaacatcc gcaacccggt
      181 ctgggtgttc ttcgggctca cccagccgat cctctacctc gtcctgttcg gccctctgct
      241 caccaacgtc agcggcggcg ggctcggcgg tgacgagtcg tggctcatct tcgtgcccgg
      301 gctgctgctg cagctgacca tcttcggggc cggcttcgcc gggttcggca tcatccagga
      361 gctgcgcgaa ggcgtggtcg agcgtcagcg ggtcaccccc gcccaccgct cggcgctgat
      421 cctcggacgc tccctgtcca acacggtgac gatcggggtg caggcggtca tcctcgttct
      481 ggtcgccatc ccgttcgggc tgcggccgtc gtggtgggga gtggtcgtgt cggtggtcat
      541 ggtcagcgtg ctcagcctgg gcctggccgc ggcgagctac gccatgggtc tgatcctcaa
      601 ggacgaggac tccttcgccc cgttcgtgca gggagtgtcc ttgccgctgc tgctgctgtc
      661 gggggtgttc ctgccgatga cgttggcgcc ggcgtggctg gagaagatgt cgcaggccaa
      721 cccgctgacc tacgtgctcg acgggacccg atcgctgttc gccggggact ggggtgcccg
      781 ggcgatctcg gtggggatcg tctgcacggt ggtggtgacg gtcgctctga cctggtggag
      841 cgcccgacgt ttccagcgcc tgtcgtcctg acaccccttc cttcccgttc tgttcgttgg
      901 atcacacctc tgacgttgtg atccaacgaa cagaacgggg gtcagggccg gggggacacg
      961 gtcaggcgag gtcgaggtcg gggtaggcgc ccagcgccgg cgccccgccg gtgtggacga
     1021 acaccgtcac gcccggcccg agcgtcccgg cgcgcgcccg ggcgacgagc gcctcggcag
     1081 ccttgccggt gtacacggga tcgaggacca gcgcctcggt ccgggcgaag agccgcatcg
     1141 cgtcgagggt tcccggcgag ggcagtccgt agccggctcc gaggtgggcg tcgtcgagga
     1201 ggacctcgtc gaaaggcacc ggcggtgttc ccagcaggtc ggccacgccg acgagcagcc
     1261 cctcgaccgc gtcgcgggtg tgctcggcgt ctcggtagac gcagatgccg tggagtcgac
     1321 tgccgatgcc cgacaggcgc agtccggtca gcaacccggc gtacgtgccg ccggtcgacg
     1381 cggcgaccac cacgtcgtcg acggtcagac ccgaggcggt gatctgctcg gcgagctcca
     1441 cgcccgcagc cacgtagccg agggtgccga cggcgtcgga cccgccgggc gggacccatc
     1501 gggcgacact gccccgggcg gcgatctgct cgtggagcgt gcccatggcg gcggcgatgg
     1561 cgtcgtcgtc gtcatcgacc accgtcaccg tggcgccgaa caggtggtcg agcgccaggt
     1621 tgccgccgtg ttcgaacgcc tcgccggagc gggcgacggc acgggtgagg atcagctcgc
     1681 agcgcaggcc gtcggccgcg cacgccgcgg cggtctggcg ggcgtggttg gactgcagcg
     1741 ctccgaacgt gacgatcgtg tcgcagccgt cggcgcgggc ccggcccagc aggaactcga
     1801 gcttgcggac cttgttgccg cccaggccga aaccggtgag gtcgtcccgc ttgacccaca
     1861 cctcctgccc cagaccctcg gacaggcgag gtgccgcccg gagcggcgtg ggtgagtcgg
     1921 cgagcggctc gcggggatgt cgcgccagct gtcgcgccag cgccccgacg ggatcgacga
     1981 cttcggtcat ccccggtgcc taccaccgtt ctgtagtgcg agatccaggc gaacgcctgg
     2041 atctcgcact acagaacggg tcggtaggct ggcccgacac cgcacgtcgc cccgacgagc
     2101 ggcgccgtcg acgaactcga cggaacccga cgaccaggaa gagacccatc cgtggacgtg
     2161 cgcatcggcg tgacccactc cccccgggag atagacctcc agctggccga cgacgtcgac
     2221 cgtgacgagc tgcgctctcg catcgacgag gtcctggccg acgactcccg agtcctgtgg
     2281 ctgaccgacc gcaagggtgc cgaggtcggc atcccgtcgg cccgcatctc ctacgtggag
     2341 atcggctcat ccgacgaggg tcggagcttc ggcttctcca gcacctgagg gcgaccgcgc
     2401 accgagatga cgaccgcacc gagatgacga ccgcaccgaa ggcagcgaac ccgtgagcgc
     2461 cggcccggtc ggcctcctcg atcgcaagct cctcttcgtc accggcaagg gcggggtcgg
     2521 caagacctcg gtcgcgtcgg ccattgcgat gctcgcggcg tcgaggggca agcggaccct
     2581 ggtctgcgag gtcgacgcca agggcaacct ggcggacttc ctcggcgtcg gaccgctgcg
     2641 cttccagccg accgaggtgc gcaagcgcct cttcgccatg tcgatggaca ccgaggagtc
     2701 gctcaaggag tacatgcggc tgaacctcaa gctgccgctg ctggccaaga tgggcccgct
     2761 ggcgcgcacg ttcgagttcg tcgccaacgc tgcgccgggg gtcaacgagc tgctgacggt
     2821 cggcaagttc acctgggagg tccgcgagcg caactacgac ctggtggtcg tcgatgcgtc
     2881 ggccaccggt cacgtcgtcg gccagctcgc cgcgccggtc gccatcaacg acctggtcaa
     2941 ggtcggactc gtcaggagcc agaccgactg gatgatcgac atcctcaccg acgccgacac
     3001 gaccggagtg gtggtggtga ccgtgcccga ggagatgccg gtgctggaga ccctcgagct
     3061 catcgagcgg ctgcggggcg agaccgagat cgacgtcgcg tcggtggtcg tcaaccgagt
     3121 ccttcccgag ctgttcggcc gcgccgagga ggaggtcttc gaacggctcc gcaagcccga
     3181 cggtgtcgcc gcgctgtcgg cccgggtcgg tcgagtggcc gcaggcaccg gagctcccga
     3241 cgacgtggcc gaccatgatg tggtccacga cgactcggcc ggcgtcgacg aggtgctcga
     3301 cggcgcggag ttggccgtca gcctgcgccg cagccgggtg ccgcacctcg accgcctccg
     3361 ggacggcctg cccgccggtc tgcccgtgct ctacctgccg tacctgttcc accgctccca
     3421 cggggtgcgc gccacggccc gcatcgccga gggcctgggc gaggagctcg ggttctgatg
     3481 gcgccgagga gcagcacgga gcggtcagac gtcgtggccc cggcgacgat cgagcagctg
     3541 tgctcggcca aggaggtcgt cgtgtcgacc ggctccggcg gcgtcggcaa gacgaccacc
     3601 gccgccgcca tcggtgcctc ggccgccacc cacctgggcg gcaaggtgct ggtgctgacc
     3661 gtcgaccccg ccaagcggtt ggccaacgcg ctcgggctcg agcgcttcgg caacgtcgag
     3721 acccaggtgc cgcccgaagc gttcacctcg gccggcgtcc aacccgaggg cgagctgtgg
     3781 gccgccatgt tggacaccaa gcagtcctgg gacgacctgg tccgcctgca cgcccccgac
     3841 gccgccaccc gcgacgcgat cctggtcaac ccgctgtacc agaacatcac cggcaagttc
     3901 gtccagagcc acgactacgt ggccatggaa cgcctctacg agatccacac ctcgggccgc
     3961 tacgacctga tcgtggtgga cacgcccccc acccgcaacg cggtcgactt cctcgacgcc
     4021 ccggaccgga tggcggactt cttctcgtcg cgactgctgc gctggctgat caccccgtac
     4081 cgttcccggc tggtgagctt cgcctccaag ccgttctact cggtcgccga ccggatcctc
     4141 ggcacgcagt tcctggagga catcgccgag ttcttcatgc tcttccagac gatgtacgac
     4201 ggcttcgtcg agcgggcccg ggccgtgacc acgctgctcg gcgggccgca gacgacgttc
     4261 gtcgtggtgt cgaccctgga ggtcgcgccg gcccgcgagg cggagtactt cctgaagctg
     4321 ctgggcgagc gcggctacca cgtcggcgct ctggtgttga acaaggtgct gccggagctg
     4381 ttcctggacc gcgacgccac cacggtggcc aagacgctcg cccatcacag cgccgaggtc
     4441 gcggccgacg tcggagcccg catcggcccg cccgcggatg acgagcgcct gctcaccacg
     4501 gtgctgggcg aggtggggga gagcttcctc aactaccagg tggtcgcccg ccgcgaggcc
     4561 gagcagcgcg ccgagctcgc ccgcatcccg caactgacgg cgaccgtccc gtacttcgac
     4621 gacgacatcc actccctcgg cggcctcctg cggctcggcg agcggatctg gagnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacgacg agacgagctt cacagccgag
     4801 cacgtcgccc tggtcgtccc cccggtcgac ccggaggcgt cccggcacct gaagcgcctg
     4861 gtcgccggct gggggatgct ggccgacttc gcgttcgccg acctgctgct ctacgtcgcc
     4921 ctgccgtcac ccgacgccgg gccggagcgc tacctggtcg tcaaccaggt gcggccgaac
     4981 accggtcaga ccctgttcaa cgacgacgtc gtcggacgga ccatgagccc ggcgcagcgc
     5041 ccgctggtcg cccgggcccg ggccgagtcc gagatctgcg aggacgtcat cgacagcccg
     5101 tggctggggg agcgcatccg cgtcaccgcg atcccggtgc ggttcgaggg ccgcgtggtg
     5161 gccgtcctgg cccgcgaggc gtcgctcgag ggggcccgtg acgccagcga cctggcgagc
     5221 gtctacttgg gggtgttccg ccgactcgga cgcatggtcg ccgacggcgt gttccccttc
     5281 gtcgaggagg aggtcatcgc caccggaggg ccgcgggtcg gtgacggcgt gctgctgctc
     5341 gacgagatgg gccgggtcgc gttcgcgtcg cccaacgcgg tgtccgccct gcggcgcctc
     5401 ggcgtgcagc ggcgcctgat cggtgagcac ctcagcgagc tcggcgccga gaccgccgcg
     5461 acctaccgcg cctacttcaa cggtcgaccg accgtggaag aggtggagcg tgacgacgtc
     5521 agcgtggtgc tgcgctgcat cccgctgatc accgacggcc gcgtcgacgg cgcggtggtg
     5581 ctcatgcgcg acgtgtcgga gatgcgctcg cgggatcggg cgctggtgtc caaggacgcg
     5641 acgatcaagg agatccacca ccgggtcaag aacaacctcc agacgatctc gtcgctgctg
     5701 cgcctccagg gccgtcgcct gcacgagccg tcggccaagg ccgcgatcga ggagtcggtt
     5761 cgccgcatcc gctcgatcgc gctggtccac gagacgctgt cgcgcgagga cggcgacgac
     5821 gtcgacttcg gcgaggtcgt gcggtccctg gtccggatgg tggaggaggg cctgacctcg
     5881 cccgacacgc cggtcgagct ggtcatcgag ggcggggccg gcaagctgcc ctcgcccgag
     5941 gcgacctcgt tggcggtggt catcaccgag ctgctccaga acgtggtgga ccacgccttc
     6001 cggcccggga cgttgaaccc gggcgaacgg ggccggatcc gcatcgtgat ggcccacgac
     6061 caggtggtgc tgcgtctggc ggtgatcgac gacggggcgg ggttccccga cgacttcgat
     6121 cccgagacct ccgacagcct gggtctgtcg atcgtccggg gtctgatcga cgagctgggc
     6181 ggcaccatgg agttcaccgc cgtccgcccg ggggagcgac ggcccggcaa ccgggtgcag
     6241 ctgaccctgc cggtggtcag ccgggtcgag gcggacgcgc aacggcctcc cccgggggga
     6301 ggccgttcgg tcggtctcta gctgtggaga ggtcgggtca ggaggcctgg gcctgcttgc
     6361 gctgggccgc gacccaggcg cggcgcagct tgcggcgctc ttcttcggag gtgccacccc
     6421 agacgccgga gtcctggttg gtcgacaggg cgaactgcag gcacggctcc ttggcctcgc
     6481 aggtgtcgca gaccgccttg gccgccgcga tctgctcgag cgccgggccc gtggttccga
     6541 ccgggaagaa cagcgacggg tcggtgtcgc tgcaggccga ggcggctcgc cagtcgtcgg
     6601 tcgccaggtt caggctgcgg ctcgaggtca gtgccacggt tcctccgtcg gatggtcggg
     6661 ggcgcgtcgg ttgcggtccc gctacgttgc ggtcacggac ttcgacgtcc cgtccgtcgg
     6721 tgtcggagcc gcgggcccgg gcgctgcacg atggagggct cctccgttcg gcatcgtccc
     6781 ggttccgcat tccgcccgga ccgggtcggc aacttgtgaa tccgtgaaca aagtcgaacc
     6841 tacagccgac cactctctgt gtaaaggaaa agtttcggga agatgtccac gacccaccga
     6901 cctctcgtca tcgcccaccg gggtgcatcc gccgatgagc ccgagaacac ggcgtccgcc
     6961 ttccgcgccg cggccgagca gggcgccgac tgggtcgagc tcgacgtgcg cctggccgcc
     7021 gacggctcgc tcatcgtcaa ccacgatgcg tggtatcggg atgagcgaac ggtgtggtcc
     7081 accccgttcg accaggtgcc gccggacacc ctcgacctgg ccgccgccct ggccgcgtgc
     7141 ggcgacatgg gggtgaatat cgagatcaag aacagtcccg gggacctggc cgacggacca
     7201 tggagcctgg aggtggtcga tgtcaccgtc gagctgatcg ggacgctcga tgcggcggcc
     7261 cgcgaccgga tcctggtgtc
//
LOCUS       DAOQOD010000156         7273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3883_length_7273_cov_12.684539, whole genome shotgun sequence.
ACCESSION   DAOQOD010000156 DAOQOD010000000
VERSION     DAOQOD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_7273_cov_12.684539"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLZ72_12895"
     CDS             <1..753
                     /locus_tag="PLZ72_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA37241.1"
                     /translation="VTSSRPIGTLVNTHANGDHCYGNGLVAGVEIVASSAAAAEMDEV
                     PPALLGAMVDSDLGDDDLNRYVRAAFGPFDFAGARPAAPTRTFDGTLGLDVAGTPVEL
                     IEAGPAHTAGDVIVHVPSAGVVFVGDLGFIGGTPIVWAGPLSNWCDAIDLVAGLGVHT
                     IVPGHGPVCGVAELLEVRDYLSFVEREATARFEAGMGVEDAIADIDLGPFAELSERER
                     LVVNVHTAYRHLDPTTPGPDVIELFRQMATYR"
     gene            complement(775..1395)
                     /locus_tag="PLZ72_12900"
     CDS             complement(775..1395)
                     /locus_tag="PLZ72_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37242.1"
                     /translation="MTIDADLSRATAAVTVPRCPDCAADLVLGGDPSVNGPGWFDHWS
                     CPAGHGLAMTLSESYERLQSDEIGQIWSRARAAAPGSRPSPFTGAPMARVTVEADDDE
                     VPDGDAGDGATTTTIVVDVDVDNQFIWLDAGELDEFPEDLPDAPVPAEVLATEHEIVA
                     RFGQAYEDAVEDRESRELTERLYRRVARHPGALRALDSIGRGLTAY"
     gene            1485..2330
                     /locus_tag="PLZ72_12905"
     CDS             1485..2330
                     /locus_tag="PLZ72_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA37243.1"
                     /translation="MTDDGSSAVRVDRDGPVTIVTIDRPRARNAVDGPTAALLAAAFR
                     DFDTDPDAAVAVLTGADGTFCAGADLKGVAEGRGNRVSSDMADDGPMGPSRMVLSKPT
                     IAAVEGYAVAGGLELALWCDLRVAATDATFGVYCRRWGVPLIDGGTVRLPRMIGHSHG
                     LDLILTGRGVGGEEARTMGLANRLCPPGEALATAVALAHQLAAFPQECLRSDRRSSYE
                     QWDLPFEAAMARETELGLGPIRSGETLAGAARFAGGEGRGGVGVDPAPAVPPPIPPVD
                     EEPRS"
     gene            2327..4288
                     /locus_tag="PLZ72_12910"
     CDS             2327..4288
                     /locus_tag="PLZ72_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNA37244.1"
                     /translation="MTDPDLSPKPATPITAAANRAVLDALPFGDRTDFELAERGRLAS
                     SPRRQITNAGGGVTWDLDSWSFLDGDAPDTVNPSLWRQSQLCAIEGLFEVVDGIYQVR
                     GFDLSNITFVRGERGWIVVDPLTSAETARAALDLVTDELGERPVTAVIYTHSHVDHFA
                     GVRGIVSDADVAEGRVRVIAPAGFLEAAIAENVTAGNVMTRRATYMYGILLPRGPQGL
                     VGSGLGQTTPLGTFGLIAPTELIAETGTELVVDGVRIEFQITPGTEAPAEMNFLFPDH
                     GALCMAENCTAVFHNVYTPRGALIRDALAWSTYLHESLELYADRAEVTFASHHWPRWG
                     AEEMRRHLSRQRDLYRYLHDQTMRLANHGLTSLEIAEELRLPASLADEFFNRDYYGTL
                     NHNVKAVYQRYLGFFDGNPAHLHPLPPVEAGRRYVEFMGGAEVLLERARASFEAGEYR
                     WVAQVVDHLVFADPDNMEARALQADALEQLGYQAESGPWRSFYLTAAQELRQGPPPLV
                     VRSAEKPDVMAAMTVEMLLQFVGVHLNGPAAAGTRLAFTLEVTDAGTDAGTGAASGGG
                     AGEVHAVGLENAALHHSAGGPHPDAEATVRTTHAGLVAAITAGRLDHLADPATEAGAV
                     SSVEGRVEVLAELSDLLDDFELFFPIVTP"
     gene            4506..5156
                     /locus_tag="PLZ72_12915"
     CDS             4506..5156
                     /locus_tag="PLZ72_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37245.1"
                     /translation="MTDLQPNQFVERATAELEKLVNDAREGARSAEELVTGSIENLQQ
                     LVEDLVEWVTEEIDKVRSQADEQRGRLESQFNDMVGSVRKPVKRAAGRASKAAERATD
                     TATKTAKGAASSAVGAVRDATGRAKPKAAKKPATAKKPATAKKASAKKKAAAKKSTAR
                     KAPAKKKAAAKKAPAKKRATTKKAPATGASTATTAAPAATPAAPPSAGSTPTHSSD"
     gene            5732..6088
                     /locus_tag="PLZ72_12920"
     CDS             5732..6088
                     /locus_tag="PLZ72_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37246.1"
                     /translation="MFGPGTFIFGVVIPLSVVALVAYGIWELIRSRDTAPAMAGAAGP
                     AASASARTILDERFARGEVDVDEYVRRRALLDGSVPSPPVDVVPATGAVVGEADTDVA
                     RAVDVDPDDGSETTGS"
     gene            6162..7055
                     /locus_tag="PLZ72_12925"
     CDS             6162..7055
                     /locus_tag="PLZ72_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA37247.1"
                     /translation="MADTNERDRPDWLERALAVEAEQSSVEVDGCDINVLAWGERGLP
                     GIVLVHGGAAHARWWAPIAPLIGEYRVVALDLSGHGDSGRRPEYRVDLWTEEVLAAAD
                     FSGASGAPVVVGHSMGGFVAVATAVAGGERVAGTIVLDSPMVEDDPEVEAARIGAAFG
                     VPKTYPSLDAAVARFRTVPAQDHYDPYILDYVARHSLRQVDDGWTWKFDPGLFRAISR
                     TAAAEQLPRITSRVALVRSEYGLVTPEIGQIIYDNLGRVAPVVEIPLAGHHMMLDQPL
                     ILLTALRALLADWEHSVPLHR"
BASE COUNT         1051 a         2557 c         2643 g         1022 t
ORIGIN      
        1 gtgacgtcgt cgcggccgat cgggacgctg gtcaacaccc acgccaacgg ggaccattgc
       61 tacggcaacg gcctggtcgc cggtgtcgag atcgtggcgt cgagcgcggc ggcggccgag
      121 atggacgagg tcccgcccgc gctgctcggc gcgatggtcg actccgacct cggcgacgac
      181 gacctcaacc gctacgtccg ggccgcgttc gggccgttcg acttcgccgg ggcgcggccc
      241 gcagcgccga cccggacctt cgacgggacg ctgggactgg acgtcgcggg aaccccggtg
      301 gagctcatcg aggcgggacc ggcgcacacc gcgggcgacg tgatcgtgca cgtgccgtcg
      361 gctggtgtgg tgttcgtcgg cgacctcggc ttcatcggtg ggacgccgat cgtgtgggcc
      421 ggtccgctgt cgaactggtg cgacgccatc gatctggtcg ccggtctggg ggtccacacg
      481 atcgtgccgg gtcacggtcc ggtgtgcggc gtcgccgagc tgctcgaggt ccgcgactac
      541 ctgagcttcg tcgagcgcga ggcgacggcg cgcttcgagg cggggatggg cgtcgaggac
      601 gccatcgcgg acatcgacct gggtccgttc gcggagctgt ccgagcggga gcggctggtg
      661 gtgaacgtcc acaccgccta tcggcacctc gaccccacga cgccggggcc ggacgtgatc
      721 gagctgttcc gccagatggc gacctaccgc tgagccgtcc ggcggcggga ccgttcagta
      781 ggcggtgagt ccccgcccga tcgagtcgag ggcccgcagc gccccggggt gacgggcgac
      841 gcggcggtac aggcgctcgg tcagctcccg cgactcgcgg tcctcgacgg cgtcctcgta
      901 cgcctgaccg aaccgggcga cgatctcgtg ctcggtggcc aggacctcgg cggggaccgg
      961 ggcgtcgggg aggtcctcgg ggaactcgtc gagctcgccg gcgtcgagcc agatgaactg
     1021 gttgtcgaca tcgacgtcga cgacgatggt cgtggtcgtc gcgccgtcac cggcgtcgcc
     1081 gtcgggcacc tcgtcgtcat cggcctcgac cgtcacccgt gccatcgggg cgccggtgaa
     1141 cgggctgggt cgcgatccgg gcgcagccgc ccgggcccgg ctccagatct ggccgatctc
     1201 gtcggactgg agccgttcgt agctctccga caacgtcatc gccagaccgt ggccggcggg
     1261 acaggaccag tggtcgaacc accccggccc gttcaccgac gggtcgccgc ccaggaccag
     1321 gtcggcggcg caatcgggac agcggggcac cgtgaccgcc gcggtcgctc gggagaggtc
     1381 ggcatcgatc gtcatgtcgg tccacggtaa cgacgtgatc gtggaattcc accggtgctc
     1441 gccgccacgg ccggccctcg agcggtcgca ctagcgtcgc ccgggtgacc gacgacggat
     1501 cgagcgcggt gagggtggac cgggacggac cggtcacgat cgtgaccatc gaccggcccc
     1561 gggcccgcaa cgccgtggac ggacccaccg ccgcgctgct cgccgcggcg ttccgggact
     1621 tcgacaccga cccggacgcg gccgtcgcgg tgctcaccgg cgccgacggc acgttctgcg
     1681 cgggagcgga tctcaagggc gtcgccgagg gccgcggcaa ccgggtgtcg tccgacatgg
     1741 ccgacgacgg gccgatgggc cccagccgga tggtgctgtc caaaccgacc atcgccgcgg
     1801 tcgagggcta cgccgtggcc ggcggactgg agctggcgtt gtggtgcgac ctgcgcgtcg
     1861 cggccaccga cgccaccttc ggcgtctact gccggcgctg gggagtgccg ctgatcgacg
     1921 gtggaacggt ccgcctgccc cgcatgatcg gccacagcca cggcctcgac ctgatcctca
     1981 ccggccgggg tgtcgggggt gaggaggccc ggacgatggg cctggccaac cggttgtgcc
     2041 ctccgggcga ggcgctcgcc accgcggttg cgctggccca ccagctcgct gcgttccccc
     2101 aggagtgcct gcgttcggat cgccgttcca gctacgagca gtgggacctc ccgttcgagg
     2161 cggcgatggc acgcgagacc gagctgggtc tgggccccat ccgctccggc gagaccctgg
     2221 ccggcgcggc ccgcttcgcc gggggcgagg gccgaggcgg tgtcggcgtc gaccccgctc
     2281 ctgccgtgcc gcccccgatc ccacctgtcg acgaggagcc ccgatcgtga ccgaccccga
     2341 cctgtcgccc aagccggcga ctccgatcac cgcggcggcc aaccgcgccg tcctcgacgc
     2401 cctgccgttc ggggaccgca ccgatttcga gttggccgag cggggccgtc tggcgagctc
     2461 tccgcggcgg cagatcacca acgcgggtgg cggcgtcacc tgggacctcg actcgtggag
     2521 cttcctcgac ggcgacgccc ccgacacggt caacccgagc ctgtggcgcc agtcgcagct
     2581 gtgcgcgatc gagggtctgt tcgaggtggt cgacggcatc taccaggtgc ggggcttcga
     2641 cctgtccaac atcaccttcg tgcgtggcga gcggggctgg atcgtcgtcg acccgctgac
     2701 gtcggccgag accgcccgcg ccgcgctcga cctggtcacc gacgagctgg gggagcggcc
     2761 ggtcaccgcg gtgatctaca cccacagcca cgtcgaccac ttcgccggtg tgcgcggcat
     2821 cgtcagcgac gcggacgtcg ccgaggggcg ggtgcgggtc atcgctccgg ccgggttcct
     2881 cgaggccgcc atcgccgaga acgtcaccgc cggtaacgtc atgacccgcc gggccaccta
     2941 catgtacggc atcctgttgc cccgcggtcc gcagggcctg gtcggaagcg gtctgggtca
     3001 gaccaccccg ctcgggacct tcgggctgat cgctccgacc gagctgatcg ccgagaccgg
     3061 caccgagctg gtcgtcgacg gggtgcgcat cgagttccag atcacgccgg gcaccgaggc
     3121 gccggccgag atgaacttcc tgttccccga ccacggcgct ctgtgcatgg ccgagaactg
     3181 cacggcggtg ttccacaacg tctacacgcc gcggggcgcg ctgatccgcg acgcgctggc
     3241 gtggtcgacg tacctgcacg agtccctcga gctgtacgcc gatcgggccg aggtcacctt
     3301 cgccagccac cactggccgc ggtggggcgc cgaggagatg cgccggcacc tgtcacgaca
     3361 gcgcgacctg taccgctacc tgcacgacca gacgatgcgg ctggccaacc acgggctgac
     3421 cagcctcgag atcgccgagg agctccggct gccggcgtcg ttggccgacg agttcttcaa
     3481 ccgcgactac tacggcacgc tcaaccacaa cgtgaaggcc gtctaccagc ggtacctcgg
     3541 gttcttcgac ggcaacccgg cgcacctgca cccgctgcca ccggtcgagg ccggccgccg
     3601 ctacgtcgag ttcatgggcg gcgccgaggt gctgctggag cgggcccggg ccagcttcga
     3661 ggcgggggag taccgctggg tggcgcaggt ggtcgaccac ctggtgttcg cggatcccga
     3721 caacatggag gcccgggcgc tccaggccga cgcgctggag cagctcggct accaggccga
     3781 gtcggggccg tggcggtcct tctacctgac cgcggcgcag gagctgcgcc agggtccgcc
     3841 gccgttggtg gtgcgcagcg ccgagaagcc cgacgtgatg gcggcgatga ccgtcgagat
     3901 gctgctgcag ttcgtcggcg tccacctcaa cggccccgcc gccgcgggca cgcgcctggc
     3961 gttcaccctc gaggtgaccg acgccggtac cgacgccggt accggcgccg cgagcggtgg
     4021 cggcgcgggc gaggtccacg ccgtcgggtt ggagaacgcc gcgctgcacc actcggccgg
     4081 cgggccgcac cccgacgccg aggcgacggt gcgcaccacg cacgccgggc tggtcgcggc
     4141 gatcaccgcc gggcgtctgg accacctggc cgatcctgcc accgaggccg gtgcggtcag
     4201 ctcggtcgag ggtcgggtcg aggtgctcgc cgagctgtcg gacctgctcg acgacttcga
     4261 gctcttcttc ccgatcgtca ccccctgacc caccccaccc cgggtttctg acgacaagtc
     4321 acgacactgg cggtactcaa ctgtcatcgg aatcgtcgat gtcctccgcg tcggtagctc
     4381 cccaggtgtt gcacacgaca cacgcggatc cgatcccagg gctttcgccg acgggcgggc
     4441 gcggcgagac tcttcggacg gtccgcgaca tcgaggaagc gcggcgacaa cccagcgagg
     4501 taccgatgac agacctgcag cccaaccagt tcgtcgaacg agcgacggcg gagctggaga
     4561 agctggtgaa cgatgcccgc gagggcgccc gttccgccga ggagctggtg acgggctcca
     4621 tcgagaacct ccagcagctg gtcgaggact tggtcgagtg ggtgaccgag gagatcgaca
     4681 aggttcgttc ccaggccgac gagcagcggg gccgtctcga gtcgcagttc aacgacatgg
     4741 tgggtagcgt ccgcaagccg gtgaagcgag cggcgggccg ggccagcaag gcggccgaac
     4801 gcgccaccga cacggcgacc aagaccgcca agggcgcggc gagctccgcc gtcggcgcag
     4861 tgcgggatgc cacgggtcgt gccaagccca aggcggccaa gaagcccgcg acggccaaga
     4921 agcccgcgac ggccaagaag gcgtcggcca agaagaaggc cgccgcaaag aagtcgacgg
     4981 caaggaaggc gccggccaag aagaaggctg ccgccaagaa ggcgccagcc aagaagcggg
     5041 cgacgaccaa gaaggctccg gccaccggtg cctccacagc cacgaccgct gcgcccgcgg
     5101 ccacgccggc agctcctccg tcggccggct ccacgccgac ccactcatcg gactgatcac
     5161 caagtcgaac tgatcgactc gggggcgccg tgcgggcggc gcccccgagg ttcgtacccg
     5221 gctctcgtcg ctctctcacc gtcctctcac ccgggcggtc cacgctgggg tcatggacga
     5281 agcgggtcgt cgagcagcac gacgtagagc ggtgattgcc ttcatcgtcg ccggggttct
     5341 ggtgatggtg agcgtggccg gactgatggc ggtcgaccgc cgtcacgacc ggttcgaacg
     5401 gatccgggac cgggtcgagg acgaacgtgg ccgctggggt gaccggatgg gaccacgccg
     5461 tggtccgggt tccgacggcg atcagagcgg ccggtgggat cagggaccga accggcaggg
     5521 cgagataccc ggccgcggcg atcacatggg tccgcgcagc gggcctgctg acgccggagg
     5581 cgccaccggt gacggcaacg ccgagcaggg tcagcgtcgg cgccatcagc ccggtaccga
     5641 ctcgtccggc ggaccgtcgt caccgacgcc gagcacgacc gtaccgacca cgaccgcacc
     5701 ggccccggcc ggcggcatct gaggaggcgt catgttcgga ccgggaacct tcatcttcgg
     5761 tgtcgtcatc ccgctctcgg tggtggcact ggtggcgtac ggaatctggg agctgatccg
     5821 ctcccgggac accgcaccgg caatggctgg agccgccggg cccgccgcgt cggcctcggc
     5881 caggacgatc ctcgacgagc ggttcgcccg cggcgaggtc gacgtcgacg agtacgtgcg
     5941 acgccgggcg ctgctcgacg gcagcgttcc gtcgcctccg gtcgacgtcg ttccggctac
     6001 gggagccgtg gtcggtgagg ccgacaccga tgttgctcgg gccgtcgacg tcgaccccga
     6061 cgacgggtcc gagacgaccg gctcctgacc ggctcgttcc agcggcccgg ctgcgtcggg
     6121 cgccgtcgtg ggccgagtcg agcgggcggt aggttcggac ggtggccgac accaacgaac
     6181 gagaccgacc cgactggctg gagcgggccc tcgctgtcga agcggagcag tccagcgtcg
     6241 aggtcgacgg atgtgacatc aacgtcctgg cctggggtga gcgcggtctg ccgggcatcg
     6301 tcctggtaca cggcggcgcc gcccacgctc ggtggtgggc gccgatcgca ccgctgatcg
     6361 gcgagtaccg ggtcgtggcg ctcgacctgt ccggccacgg cgacagcggc cgacgacccg
     6421 agtaccgggt ggacctgtgg accgaggagg tcctggccgc cgcggacttc tccggtgcat
     6481 ccggtgcgcc ggtggtggtc ggacactcga tgggcgggtt cgtggccgtg gccacagccg
     6541 tcgcgggcgg ggagcgggtg gccggcacga tcgtgctgga ctcgccgatg gtcgaggacg
     6601 accccgaggt ggaagcggcc cgcatcggtg ccgcgttcgg cgtgcccaag acctatccga
     6661 gcctcgacgc cgcggtcgcc cggttccgga ccgtgccggc ccaggaccac tacgacccgt
     6721 acatcctcga ctacgtcgcc cgtcactcgc tgcggcaggt cgacgacggc tggacctgga
     6781 agttcgaccc gggcctgttc cgggcgatca gccggaccgc tgcggccgag cagctgcccc
     6841 gcatcaccag ccgggtggcg ctggtgcgtt cggagtacgg cctggtgacc cccgagatcg
     6901 gccagatcat ctacgacaac ctgggtcggg tggcgccggt ggtggagatc ccgctggccg
     6961 gacaccacat gatgctggac cagccgttga tcctgctcac ggccctgcgg gcgctgctgg
     7021 cggactggga gcactcggtc cccctccacc gctgagcccc acatcccttc ccgctgcgtt
     7081 ctgacgataa gtcacgacgc tggcggtact caactgtcat gggaaccggg aggcggggct
     7141 tccaggccgg tggttccgac tggctgggtc ggcgagcagg ggctatcgtg actgtcacgc
     7201 cgcggggtgg agcagttcgg tagctcgtcg ggctcataac ccgaaggtcg caggttcaaa
     7261 tcctgccccc gcc
//
LOCUS       DAOQOD010000157         7251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3899_length_7251_cov_12.022235, whole genome shotgun sequence.
ACCESSION   DAOQOD010000157 DAOQOD010000000
VERSION     DAOQOD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_7251_cov_12.022235"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLZ72_12930"
     CDS             complement(<1..632)
                     /locus_tag="PLZ72_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HNA37248.1"
                     /translation="MPDDLPADAVPIRMLGPDDTGPGSVDPGTVDPGTVDPDTVDHQI
                     LDPGAGGGGPSGRVVVALVAVLAVLVGVAGVLVYQRATTPGADSVDVGFMQDMTTHHQ
                     QAVEIASIVAETAQDPDIRAFAREILTFQSYEVGYMEALLEDWGQWPFSDDRTAMKWM
                     DMASTPSEMPGMQTEASIQQLEDATGSQVDALFIPMMIDHHRGGVHMAEYA"
     gene            766..1428
                     /locus_tag="PLZ72_12935"
     CDS             766..1428
                     /locus_tag="PLZ72_12935"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNA37249.1"
                     /translation="MARTRTSPGRAGLGIVATASLVVIALGLLALVVVGLLNVWNPFG
                     TTTVDRSGPAVLDRIRTLEEFTAAEANFTQDVDLENDNFLPGFIKGERVTAIVTGNVR
                     ATVDFGDLGPNSVKVSNDGTSIQLTLPEPQLTDPEIDVGSARIVSRDRGIVDRIADVF
                     ESNPIDDGPVYRAAEKKLQAAARNSDVLEQARTNTEQWLRTFLGAAGFDEITINWAES
                     PN"
     gene            1445..2236
                     /locus_tag="PLZ72_12940"
     CDS             1445..2236
                     /locus_tag="PLZ72_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37250.1"
                     /translation="MTSPDLPPAAPTAPVPPAPAATIAWAAQVIGATKVYGVGEAEVR
                     ALDGVTVGFEPGRFTAIMGPSGSGKSTLMQCAAGLDRLTAGQAFIGDVDITRLTEKQL
                     TLLRRDRVGFVFQQFNLLPTLTARENITFPLDLAGRSPDPAWMDQVVATVGLGDRLDH
                     KPSELSGGQQQRVAVARALVERPQIIFADEPTGNLDSRSSAEILTFMARAVAEQAQTV
                     VMVTHDPVAASYAGRILFLADGRIVDELLAPTRESILARMGALGI"
     gene            2251..4824
                     /locus_tag="PLZ72_12945"
     CDS             2251..4824
                     /locus_tag="PLZ72_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNA37251.1"
                     /translation="MLRYALTNLRANLTRLISTALAVIIGIGFLAAGLMLTDAMRAAL
                     TGTVDLEYARVDLVVEPAGAIEGGPASVPGSVLDAVRATDGVAAAAGELTGPVNLLGR
                     NDESLTSRAQGRSWIDDDELDPLSIESGRAPRSATGSTTDGDGDVEVAVDRGTAEDQD
                     LEVGSHVRLATPSGPVAATVVGVTNLGGQDALDDGGTFSFAPTDATRLLAPGTGGWTR
                     ILVATDEGRTRAVADTLREDLPRSVEVQDGDRFRSDQRAMSAGLVDLLRPLLQGFAYL
                     AMFVAAFVIFNTFSVVVTQRFRELALIRAVGGTPAQVRRSLLFEGAVIGAVSSAIGIV
                     FGMGLAFAVQAILGAFDIRLPGAGLSVRPWTIVLCLTVGTVVTVLSVFVPAFRAGRTK
                     PVEAMRDAAVDHSGTSTVRAVIGGSALALGLALLLGVRLVGLTSWLLPPGALLLFVGV
                     LVGGPLVARLFAGALRVPARRLGLTARLAVDNTVRNPRRTATTANALVIGLFLVTVVT
                     VSGDALKRFTVEKIDELSASDYIVAAGANGISPELVAKVQGTDGVAAGAPIRQTVVLD
                     SSGRMVFLSGADIDLLRRSTGLAAEDGSIDAVADGTGAAVPALAGASGGGAGPGEDRS
                     MAPLPTRTGSTMELVAADGTTERVPVVATLKPELDTLLLQVVVSDERFTELAGDQPVS
                     MIYVRVDQGQADAVGRRLNRLVAGHTGVEVQPGNFLGQLVGQVFDFLINTVNALLGMS
                     VIIALVGIVNTLTLSIIERRRELGMVRALGMTRQQVGRMVRAEAVLIGTLGTLIGVGS
                     GLLLGWVLIGSLSAEIALEVNWARIALVVLLGVAAGVVASILPARRATRVEMVEAMAA
                     T"
     gene            complement(4884..6512)
                     /locus_tag="PLZ72_12950"
     CDS             complement(4884..6512)
                     /locus_tag="PLZ72_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNA37252.1"
                     /translation="MLRSVDVHDHPGREVVAGITVAAVAVPGVIGYTHIAGMPIVTGL
                     YTMIIPATVFVVLCSSRHLVVGADSTTAAILAAGLTPLAAVGSADYVHLAAMVAVLTG
                     AMLLVARLAGLGFISDFLSRTLLIGFLAGVGVQVSVGQLPAALGVPVTATGPVPTLVA
                     LVGHLSEIVAADVALSAVSIAVMAVGGWLRPQVPWPLLVVVAGIAASATGAIDVATIP
                     DVTGGLPLPSWPAAGDLDRVASLLPVALMCFVVVLAQSSATARAYAGRHGERVDTDMD
                     LVALGAANLAAGFTGTFVVNGSPTRTEVMHGAGGRNQAAQLVAAAVTVVVLLVAGPVL
                     TDLPVAVLAAVVVMIAIRLIDLPAIVDVFHRRRVEGAVVIATAVTVIAAGVGPGILFA
                     MGLSVVVHLRHSYRPNVRALGRDRDRWRMSRLEHDHQIVPGLAAVFPSSNLYYANATH
                     IADEILEMVDAARPPLRWLCVLAIAVDDVDLTASEVLGRLHGELADRGVTLVLCGLEP
                     HVRHELERDGLIERIGPQHVFDYVEDAVAAYRAL"
     gene            6567..>7251
                     /locus_tag="PLZ72_12955"
     CDS             6567..>7251
                     /locus_tag="PLZ72_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HNA37253.1"
                     /translation="MCDTMVTVTDEGVLFAKNSDRDANEAQLLEWIPARDHVRTDSAD
                     PTDSEVTVRCTWIDIAQVAHTHAVLLSRPWWMFGAEMGANEHGVVIGNEAVFTRELSR
                     RSGRVGSRHPEAEPGLLGMDLLRLALERATNATDAVEVIVTLLERHGQAGSCSRAHPN
                     FTYDNSFIVADPHGAIVVETAGRHHATEEVRSGGRSISNGLTIPGFAAADADALRARV
                     ASCAARRTRT"
BASE COUNT         1034 a         2643 c         2596 g          978 t
ORIGIN      
        1 gcgtactcgg ccatgtggac cccgccccgg tggtggtcga tcatcatcgg gatgaacagg
       61 gcgtcgacct ggctgccggt ggcgtcctcg agttgttgga tcgacgcctc ggtctgcatg
      121 ccgggcatct ccgagggcgt cgacgccatg tccatccact tcatggcggt gcggtcgtcg
      181 gagaacggcc actggcccca gtcctcgagc agcgcctcca tgtagcccac ctcgtagctc
      241 tggaaggtca ggatctcgcg ggcgaaggcc cggatgtcgg ggtcctgggc ggtctcggcg
      301 acgatcgacg cgatctcgac ggcctgctgg tgatgggtgg tcatgtcctg catgaacccg
      361 acgtcgaccg agtccgcgcc gggtgtggtg gcccgctggt agacgagcac cccggcgacg
      421 ccgaccagga ccgcgagcac cgcgaccagc gcgacgacga cccgaccgga cgggcctccg
      481 ccgccggcgc ccggatccag gatctggtga tcgaccgtgt cgggatcgac ggttccggga
      541 tcgacggttc cgggatcgac cgagccagga cccgtgtcgt cggggccgag catccggatc
      601 gggaccgcgt ccgcgggcag gtcgtcgggc accgacccag ggtagccagc acctgaaggc
      661 cacccccggg tcacccgaca cagcgcagcg aatcctgaga aggcgggcag cggcccggac
      721 cggaaccgtc ggaggggacc gaccgcccgg tacgttgggc ggaccatggc tcgcacccga
      781 acctcccccg gccgggccgg cctcggcatc gtcgccaccg cgtcgctggt cgtgatcgcg
      841 ctcggactgc tcgccctggt cgtggtgggc ctgctgaacg tctggaaccc gttcggcacc
      901 acgaccgtcg accgatcggg ccccgcggtc ctcgaccgca tccgcacgct cgaggagttc
      961 acggcggccg aggcgaactt cacccaggac gtcgacctgg agaacgacaa cttcctgccc
     1021 gggttcatca agggcgaacg ggtcaccgcg atcgtgaccg gcaacgtgcg ggcgacggtc
     1081 gacttcggcg acctgggccc caactcggtg aaggtgtcca acgacggcac cagcatccag
     1141 ctcaccctgc ccgaaccgca gctgaccgac ccggagatcg acgtcgggtc ggcccgcatc
     1201 gtgagccggg accgtgggat cgtcgaccgg atcgcggacg tgttcgagtc caacccgatc
     1261 gacgacgggc ccgtctaccg cgcggcggag aagaagctgc aggcggccgc ccggaactcc
     1321 gacgtgctcg aacaggcccg gaccaacacc gagcagtggc tgcgcacctt cctgggcgcc
     1381 gccgggttcg acgagatcac gatcaactgg gccgagtccc cgaactgacc cgcaggagag
     1441 ctccgtgacc tcccccgacc tgcccccggc cgcgcccacc gcgcccgtgc cgcccgcgcc
     1501 cgccgccacg atcgcgtggg cggcccaggt gatcggcgcc accaaggtgt acggcgtcgg
     1561 cgaggccgag gtgcgcgccc tcgacggcgt caccgtcggc ttcgaacccg gccgcttcac
     1621 cgccatcatg ggcccgtcgg gctccggcaa gtcgacgctg atgcagtgcg ccgccgggct
     1681 cgaccggctc accgccggac aggcgttcat cggcgacgtc gacatcaccc gcctgaccga
     1741 gaagcagctg accctgctgc gccgggaccg ggtcgggttc gttttccagc agttcaacct
     1801 gctgcccacc ctcaccgccc gcgagaacat caccttcccc ctcgacctgg ccggccgctc
     1861 ccccgacccg gcgtggatgg accaggtggt ggcgaccgtc gggctgggcg accgcctgga
     1921 tcacaagccg tcggagctgt ccggcggcca gcagcagcgc gtggcggtgg cccgcgccct
     1981 ggtcgagcgg ccgcagatca tcttcgccga cgagccgacc ggcaacctcg actcccggtc
     2041 ctcggccgag atcctcacct tcatggcccg ggccgtcgcc gagcaggccc agacggtggt
     2101 gatggtgacc cacgacccgg tcgccgcgtc gtacgccggg cgcatcctgt tcctggccga
     2161 cggacgcatc gtcgacgagc tgctggcccc cacccgggag tcgatcctgg cccgcatggg
     2221 cgccctgggc atctgaggcg cgctccgacc gtgctgcgct acgccctcac caacctccgg
     2281 gccaacctca cccggttgat ctcgaccgcg ctggccgtga tcatcggcat cgggttcctg
     2341 gccgccgggc tgatgctcac cgacgccatg cgcgcggcgc tgaccggcac cgtcgacctc
     2401 gagtacgcgc gggtcgacct ggtcgtcgaa ccggccggcg cgatcgaggg aggaccggcg
     2461 tcggtccccg gatcggtgct cgacgcggtc cgcgccaccg acggggtcgc cgccgcggcc
     2521 ggcgagctca ccgggccggt caacctgctg ggacgcaacg acgagtcgct gacctcccgc
     2581 gcccagggtc ggtcctggat cgacgacgac gagctcgatc cgctgtcgat cgagtccggc
     2641 cgagcgccgc gatcggccac cggcagcacg accgacggcg acggcgacgt cgaggtcgcc
     2701 gtcgaccgcg gcaccgccga ggaccaggac ctggaggtcg gctcccacgt ccggctcgcg
     2761 accccgtccg gaccggtcgc cgccaccgtc gtcggagtca cgaacctggg cggccaggac
     2821 gccctcgacg acggcggcac gttctccttc gcgccgaccg acgccacccg gctgctcgcc
     2881 cccggcaccg ggggctggac ccgcatcctg gtggcgaccg acgagggccg cacccgggcc
     2941 gtcgccgaca cgctgcgcga ggacctgcca cggtcggtcg aggtgcagga cggggaccgg
     3001 ttccgcagcg accaacgagc gatgagcgcc ggcctggtcg acctgctgcg gccgctgctg
     3061 cagggcttcg cctacctggc catgttcgtg gccgcgttcg tcatcttcaa cacgttctcg
     3121 gtggtggtca cccagcggtt ccgcgagctc gcgctgatcc gcgccgtcgg cggcaccccg
     3181 gcgcaggtcc gtcggtcgct gctgttcgag ggggcggtca tcggggcggt gtcgagcgcc
     3241 atcggcatcg tgttcggcat ggggttggcg ttcgcggtcc aggcgatcct gggggccttc
     3301 gacatccgac tgccgggcgc cgggctgtcg gtgcggccct ggacgatcgt gctgtgcctg
     3361 acggtcggca cggtggtgac cgtgctgtcg gtgttcgtcc ccgcgttccg cgccgggcgc
     3421 accaagccgg tcgaagcgat gcgcgacgcg gcggtcgacc actccggcac ctcgacggtg
     3481 cgcgccgtca tcggcggctc ggccctggcg ctgggccttg cgctgctgct cggggtccgc
     3541 ctggtgggtc tcacctcgtg gctcctgccg ccgggagcgc tcctgctgtt cgtcggtgtg
     3601 ctggtcggcg gtccgctggt ggcgcgcctg ttcgccggtg ccctgcgggt gccggcccgc
     3661 cgcctgggcc tgaccgcacg gctggcggtc gacaacacgg tgcgcaaccc tcgccgcacc
     3721 gcgacgacgg ccaacgccct ggtgatcggg ctgttcctgg tcaccgtggt caccgtgtcg
     3781 ggcgacgccc tcaagcggtt cacggtggag aagatcgacg agctgtcggc ctcggactac
     3841 atcgtcgccg ccggagcgaa cggcatctcg cccgagctgg tcgcgaaggt ccagggcacc
     3901 gacggcgtgg cggccggggc gccgatccgc cagaccgtcg tgctcgactc gtcgggacgg
     3961 atggtgttcc tctccggcgc cgacatcgac ctgttgcgac gctccaccgg gctggccgcc
     4021 gaggacgggt cgatcgacgc cgtcgccgac gggaccggag cggcggtccc cgcgctggcg
     4081 ggagcgtccg gcggcggagc cggtcccggg gaggaccgct cgatggcgcc gctgcccacc
     4141 cggacgggct cgacgatgga gctggtcgcc gcggacggga ccaccgaacg ggtacccgtc
     4201 gtggccaccc tgaaacccga gctcgacacc ctcctgctgc aggtcgtcgt gtccgacgag
     4261 cgcttcaccg agctcgcggg tgaccagccg gtgtccatga tctacgtgcg cgtcgatcag
     4321 gggcaggccg acgcggtggg acggcggctc aaccgactcg tcgccggcca caccggcgtc
     4381 gaggtccaac ccggcaactt cctcggccag ctcgtcgggc aggtgttcga cttcctgatc
     4441 aacaccgtca acgcgctgct ggggatgagc gtgatcatcg ccctggtcgg catcgtcaac
     4501 accctcaccc tgtcgatcat cgaacggcga cgggagctcg gcatggtccg ggcgctgggg
     4561 atgacccgtc agcaggtcgg acgcatggtg cgggccgagg ccgtgctgat cggcacgctg
     4621 ggcacgctca tcggcgtcgg ctccggtctg ctgctcggct gggtgctgat cggcagcctc
     4681 agcgcggaga tcgccctgga ggtgaactgg gcgcggatcg ctctggtggt cctgctcggg
     4741 gtggcggccg gggtcgtcgc atcgatcctg ccggcccgac gcgcgacccg cgtcgagatg
     4801 gtcgaggcga tggccgccac ctaggtctcg cagctccgcc gcccccaacc gcggcaccgg
     4861 gacccgacgg gacccgggcc ggctcagagc gcccggtagg ccgcgacggc gtcctcgacg
     4921 tagtcgaaga cgtgctgcgg tccgatccgc tcgatcagac cgtcacgctc gagctcgtgg
     4981 cggacgtggg gctccaggcc gcacagcacc agcgtcacgc cccgatcggc cagctccccg
     5041 tggagcctgc cgagcacctc cgacgcggtc aggtcgacgt cgtcgaccgc gatcgccagc
     5101 acgcagagcc atcgcagcgg gggccgggcg gcgtcgacca tctccaggat ctcgtcggcg
     5161 atgtgggtgg cattcgcgta gtagaggttc gagctcggga acaccgcggc caggccgggg
     5221 acgatctggt ggtcgtgttc gagtcggctc atgcgccagc ggtcgcggtc gcggccgagg
     5281 gcgcgcacgt tgggtcggta gctgtggcgg aggtggacga ccaccgacag gcccatggcg
     5341 aagaggatcc cggggccgac gccggcggcg atcaccgtca cggccgtcgc gatgacgacg
     5401 gcgccctcga cccgacgacg gtggaagacg tcgacgatgg cgggcaggtc gatcaggcgg
     5461 atggcgatca tcaccacgac cgcggccagc accgcgacgg gcaggtcggt gagcaccgga
     5521 ccggcgacca gcagcaccac gacggtgacc gccgcggcga cgagctgcgc ggcctggttg
     5581 cgtccgcccg ctccgtgcat cacctcggtt cgggtcgggc tgccgttgac gacgaaggtc
     5641 ccggtgaagc cggcggccag gttggccgcg cccagtgcga ccaggtccat gtcggtgtcg
     5701 acgcgctcgc cgtggcgtcc ggcgtacgcc cgggccgtcg ccgagctctg cgccaacacc
     5761 accacgaagc acatgagagc gaccggcagc agcgacgcga cccggtcgag gtccccggcc
     5821 gcgggccacg acgggagggg gagcccgccg gtgacgtcgg ggatcgtcgc cacgtcgatc
     5881 gcccccgtgg cgctcgcggc gatccccgcc accacgacca gcagcggcca cgggacctgt
     5941 ggccgcagcc aacccccgac cgccatcacc gcgatcgaca ccgcggagag agccacgtcg
     6001 gccgccacga tctcggagag gtgaccgacg agggcgacga gggtcggaac cggtcccgtg
     6061 gcggtcaccg ggacgccgag cgcggcgggg agctggccga ccgagacctg gaccccgaca
     6121 ccggcgagga agccgatcag gagggtgcga gacaggaagt cggagatgaa ccccaggccg
     6181 gccagcctcg ccaccagcag catcgcgccg gtgaggaccg ccaccatcgc ggcaaggtgg
     6241 acgtagtcgg cggagccgac cgcggccagc ggggtgagtc ccgcggcgag gatcgcggcg
     6301 gtggtcgaat ccgcgccgac cacgaggtgt cgtgaggagc acaggaccac gaacaccgtc
     6361 gccgggatga tcatcgtgta gaggccggtc acgatcggca tgccggcgat gtgggtgtag
     6421 ccgatcaccc cgggcaccgc gaccgcggcc accgtgatcc ccgcgaccac ctcccggccc
     6481 gggtggtcgt gcacgtcgac cgagcgcagc accgcgctca ccttctcacg tcggcgacgg
     6541 tcggacgagg cgtagggtcg gtggtgatgt gcgacacgat ggtcaccgtc accgacgagg
     6601 gcgtcctgtt cgccaagaac agcgaccgcg acgccaacga ggcgcagctg ctcgagtgga
     6661 tcccggcacg cgaccacgtc cggaccgact cggcggaccc gacggactcc gaggtgacgg
     6721 tgcgctgcac ctggatcgac atcgcgcagg tggcccacac ccacgccgtg ctgctgtcgc
     6781 ggccctggtg gatgttcggc gccgagatgg gggccaacga gcacggcgtg gtcatcggca
     6841 acgaggcggt gttcacccgg gagctctccc gtcgcagcgg acgggtcggg tcccgacacc
     6901 ccgaggccga acccggcctg ctcggcatgg acctgctgcg cctcgcgctg gagcgggcga
     6961 ccaacgcgac cgacgccgtc gaggtgatcg tgaccctgct ggaacgccac ggccaggccg
     7021 gctcgtgcag ccgggcgcac ccgaacttca cctacgacaa cagcttcatc gtcgccgatc
     7081 cgcacggagc gatcgtggtc gagaccgccg gtcgccacca cgccaccgag gaggtccgct
     7141 ccggcggacg cagcatctcc aacgggttga cgatccccgg cttcgcggcc gccgacgccg
     7201 acgcgctgcg cgctcgggtg gcgtcgtgcg cggcgcggcg aacccgcacc c
//
LOCUS       DAOQOD010000158         7248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3901_length_7248_cov_10.449326, whole genome shotgun sequence.
ACCESSION   DAOQOD010000158 DAOQOD010000000
VERSION     DAOQOD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_7248_cov_10.449326"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1552
                     /gene="wecB"
                     /locus_tag="PLZ72_12960"
     CDS             113..1552
                     /gene="wecB"
                     /locus_tag="PLZ72_12960"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNA37254.1"
                     /translation="MSEPRDRPDAHEPGRGRVTLPDGVLPPGVEKDRRSHRRHPIEIL
                     VVIGTRPEAIKMVPLVQALHDHPALHPVVISTGQHRDLVDPILAMAGITADVDLGCGG
                     PGITLNDLATSVMTGFDRYCRSRYGDPTGDVAELTHVVRDGYPGAVLVHGDTSSAMAA
                     ALSSFHLRLPVVHVEAGLRTYNTLTPFPEEMNRQIISRIACFHLAPTPLNGEHLVREG
                     VPYDRVYVCGNTGIDAMRWAASLRRVPTDPAVAAVAAGSDPLMVVTTHRREHWEGGIE
                     RVATAVATLAERRPHTRFVVPMHPNPIVRHQIVPHLGDRPNVVLTEPMAYVEFASLLA
                     RSDLVLTDSGGIQEEAPVVGTPVLVAREETERTEGVDAGTLRLVGTDPTRIVEWSERL
                     LDDTPERRHMLASENPYGDGRSAPRIADALRFLAGDDRILPEQFGPGFDRRAVMEAAG
                     YESLRHATEQSAFGAPPNEDHDAERWEHV"
     gene            1549..2916
                     /locus_tag="PLZ72_12965"
     CDS             1549..2916
                     /locus_tag="PLZ72_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNA37255.1"
                     /translation="MTAWLGLYDGAPPWAQTILLAALVVIVGCNAWVVLLFARSRAAL
                     RHLPDPDDVTAAELLWVVMVPALDEEVTIADSVGRLDALDAAHRVVVVIDDGSTDRTP
                     ELLDALRRDLPDGSLSVLRRDPPDARRGKAAALNAAWRHLHEVVLREGPFAGWDPHKV
                     VVTIVDADGRLDPQAPRFVAAHFADERVGGVQALVRIYNRHRPLTWCQDVEFGIYGEL
                     YQLGRSRWGTAGMGGNGQFNRLSALDSVVTPDDEGPWRHRLTEDQDVGLRLIRAGWHG
                     RQEVRVTVDQQGLPGLRRLYRQRTRWSQGNLQAMAQLPGVLRAPVSVGARLDLAAYLL
                     NPLWQAVVGLSLVVGLYLFAFEDVPFWSSAMPWWLLLITYSLGFTGAVLGTVARSARR
                     TPWAWLVGLVVAQPYALYSWLLFPVLIRATIRLVARRDSWAKTDRVPVGEQTVMGVGG
                     ATAAR"
     gene            complement(2900..4048)
                     /locus_tag="PLZ72_12970"
     CDS             complement(2900..4048)
                     /locus_tag="PLZ72_12970"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNA37256.1"
                     /translation="MAEAYIIDTVRTPVGKRGGSLSQIHPADLGAHTLRELVARTGID
                     AGAVDDVVMGCLDTIGSQAGDVGRTAWLAAGLPEHVPGTTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVVAAGLQNMSRIPISSAMLVAEQFGDTDPFSGSTGWVERYGDQEVSQFRG
                     ADMIAEKWEISREDMEEFAMESHRRAITARAEGRFDNEIVELAGLTFDEGPREPDPVK
                     IASLKTLREGGRITAAVASQISDASAAMLVASEQAVKDHGLTPRARIHHLSVLADDPV
                     YMLSAPIPATKRALEKAGMTLDDIDLVEINEAFASVVLAWAKELDADLSKVNVNGGAI
                     ALGHPLGATGVRLMTTLLNELERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            4239..4709
                     /locus_tag="PLZ72_12975"
     CDS             4239..4709
                     /locus_tag="PLZ72_12975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNA37257.1"
                     /translation="MKRTWRAEEFDLLSPSVAKRWSAFLNEEGDRRWFPNLLGIEVEE
                     LRRDYARLRMPWRNDLNQATGMMHGGAIATLIDTAVVPAIGTAYSDNRPFSTIEMSVR
                     YIQPVRQEDLVAEGWVTRRGKRVVFCEVEVRTVSGVTVATGDLIYVVGSPPPPE"
     gene            4883..6079
                     /locus_tag="PLZ72_12980"
     CDS             4883..6079
                     /locus_tag="PLZ72_12980"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HNA37258.1"
                     /translation="MAQRGRRRGFRVAMATIALGSIAAMACTPPGEPAGPVDPGPSVP
                     SGDDAIGLNQFQYLGSHNSYKIGPQADLLLMLGLGAAALPDLAGAGLDPAQLNYTHRP
                     LTQQLDSGIRSFELDVWADPTGGRFAAPLAAQLLGQPWPSGVDAPGFKVLHIQEIDYR
                     STCPTLDGCLQEMRAWSDAHPGHLPITINLELKADPLPAPFDVTPIQPFDTPTLDALD
                     ADLRAQIGDRLITPDEVRGTAPDLRAAVTDPDSGGGWPSVADSRGRFLLFLDNGGSVA
                     ERYLDGHPSLAGRVLFTSDGFDRDDAAVLKVNEPGDGSEISALVAQGFLVRTRADADV
                     VNPGADRTAALASGAQMVHSDFPAGEAQFGTGYQVSFPTRVQARCNPVNTTSATCTAA
                     AAVEPT"
     gene            complement(6129..6971)
                     /locus_tag="PLZ72_12985"
     CDS             complement(6129..6971)
                     /locus_tag="PLZ72_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37259.1"
                     /translation="MSDRDRDDLAARARSLLHERLDARLGERLRRDGPRPRDGAGWVD
                     VSAHDLELRCPARWSVPFDDFVLSVRTAAGAVGRLALRERREGEPVGVAVARVTAGLA
                     DADPSVAWFASWYDQELDRSGQAALRAAAITWAHGALAAVGGRSLNWATARQPHDVDG
                     RTLRLRTSWDASSSGARPEVLVVISTRPPADPAAARLAGFNALVDGLLRSRVPDRVRV
                     ASAATASTTAFPVSMDLLEPVIDRVVELVGWRVDSDSSPTVPGRWCADCHLLGICPAA
                     PEFS"
     gene            complement(6968..>7248)
                     /locus_tag="PLZ72_12990"
     CDS             complement(6968..>7248)
                     /locus_tag="PLZ72_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37260.1"
                     /translation="ATHYALLTALRDGRAPAAVARWYPGGALATMAVTSDVLESAARR
                     LGAAIDAWAELAVGRPPRERPGPACSWCPDAGVCPSFAPAPDPFGVDA"
BASE COUNT         1009 a         2668 c         2565 g         1006 t
ORIGIN      
        1 tcggaccgca ccggcccgcc ccgctccatc ggccggcagc ggtcggggtt gagcggatct
       61 cacgatcgac cgctcgcgtc gcttcagcgc gtcgcgtcga tagcgtcgag ggatgtcgga
      121 accccgcgac cgtcccgatg cccatgaacc cggccgcggt cgggtcacgc tgcccgacgg
      181 ggtgctgccc ccgggcgtcg agaaggaccg gcggtcccac cggcgacacc cgatcgagat
      241 cctggtggtg atcgggaccc gacccgaagc gatcaagatg gtgcctctgg tgcaggcgct
      301 gcacgaccac cccgcgctgc acccggtcgt gatctcgacc ggccagcacc gcgacctggt
      361 cgatccgatc ctggccatgg ccgggatcac cgcggacgtc gacctgggct gcggcggccc
      421 cgggatcacc ctcaacgacc tggcgacctc ggtgatgacc ggcttcgacc gctactgccg
      481 gtcccgctac ggagatccga ccggcgacgt cgccgagctc acccacgtcg tgcgcgacgg
      541 ctaccccggc gcggtgctcg tccacggcga caccagctcc gccatggccg cggccctcag
      601 ctcgttccac ctccggctgc cggtggtgca cgtcgaggcc ggactgcgga cctacaacac
      661 gctgaccccc ttccccgagg agatgaaccg ccagatcatc tcccgcatcg cgtgcttcca
      721 cctggccccg accccgctca acggcgagca cctggtccgg gagggcgtcc cctacgaccg
      781 ggtctacgtg tgcggcaaca ccgggatcga cgccatgcgc tgggcggcgt cgctgcgccg
      841 ggtcccgacc gaccccgcgg tcgccgcggt cgccgcggga tccgatccgc tgatggtggt
      901 caccacgcac cgacgcgagc actgggaggg cgggatcgag cgagtcgcga ccgcggtcgc
      961 gaccctggcc gaacgtcgac cccacacccg cttcgtggtc ccgatgcacc cgaacccgat
     1021 cgtgcgccac cagatcgtcc cccacctggg cgaccggccc aacgtggtgc tgaccgaacc
     1081 gatggcctac gtcgagttcg cctcgctgtt ggcccgctcc gacctggtgc tcaccgactc
     1141 gggcggcatc caggaagagg cgccggtggt cggcacaccg gtgctggtgg cccgcgagga
     1201 gacggaacgc accgagggcg tcgacgctgg cacgctgcgc ctggtcggca ccgacccgac
     1261 ccgcatcgtg gagtggagcg agcggttgct cgacgacacg cccgaacggc gccacatgct
     1321 ggcgtcggag aacccgtacg gcgacgggcg ctccgcgccg cggatcgccg acgcccttcg
     1381 gttcctggcc ggcgatgacc ggatcctgcc cgagcagttc ggaccgggct tcgaccgtcg
     1441 ggcggtcatg gaggcggcgg gctacgagtc gctgcgccac gccaccgagc agtcggcgtt
     1501 cggggcgcct ccgaacgagg accacgacgc cgaacggtgg gagcacgtgt gaccgcctgg
     1561 ctcggactct acgacggagc gcctccgtgg gcgcagacga tcctgctggc cgccctggtc
     1621 gtcatcgtcg gctgcaacgc ctgggtcgtg ctgctgttcg cacggtcacg ggcggcgttg
     1681 cgccacctcc ccgatcccga cgacgtcacc gccgcggagc tgctgtgggt ggtgatggtc
     1741 cccgcgctcg acgaagaggt cacgatcgcc gactccgtcg gtcggctgga cgcgctggac
     1801 gccgcacacc gcgtcgtggt ggtcatcgac gacgggtcga ccgaccggac gcccgaactg
     1861 ctcgacgcgc tgcgtcgcga cctccccgac ggatcgctgt cggtgctgcg acgcgatcct
     1921 cccgacgccc gtcgcggcaa ggccgccgcg ctgaacgcgg cgtggcggca cctccacgag
     1981 gtggtcctgc gcgagggccc gttcgcgggc tgggatcccc acaaggtcgt cgtcaccatc
     2041 gtcgacgccg acggtcggct cgacccgcag gccccccgtt tcgtcgccgc tcacttcgcg
     2101 gacgaacggg tggggggcgt ccaggccctc gtgcgcatct acaaccgcca ccgtccgctc
     2161 acgtggtgcc aggacgtgga gttcggcatc tacggggagc tgtatcagct gggccgcagc
     2221 cgctggggca ccgccggcat gggcggcaac gggcagttca accgcctgtc ggccctcgac
     2281 tcggtggtca cccccgacga cgagggtccg tggcgccacc gcctcaccga ggaccaggac
     2341 gtcggactgc gcctgatccg ggccgggtgg cacgggcgcc aggaggtccg cgtcaccgtg
     2401 gaccagcagg gcctccccgg gctgcgacgc ctgtaccggc agcggacccg ttggtcccag
     2461 gggaacctgc aggccatggc gcagctcccc ggggtgctcc gggccccggt gtcggtcggc
     2521 gcccgactcg acctggccgc ctacctgctc aacccgctgt ggcaggcggt ggtggggttg
     2581 tcgttggtcg tcgggctcta cctgttcgcg ttcgaggacg tgccgttctg gtccagcgcc
     2641 atgccgtggt ggctgctcct catcacctac tcgctcgggt tcaccggagc ggtgctcggg
     2701 accgtggccc gaagcgcccg ccgcacaccg tgggcgtggc tcgtgggcct ggtcgtcgcg
     2761 cagccctacg ccctgtacag ctggctcctg ttcccggtgc tgatccgggc cacgatccgt
     2821 ctggtcgccc gacgcgactc gtgggccaag accgaccgcg taccggtcgg agagcagacg
     2881 gtgatggggg tggggggcgc tacagccgct cgatgatcgt cacgtttgct tggccgccgc
     2941 cttcgcacat ggtctgcagg ccgtagcggc ctccggttcg ctccagctcg ttgagcagcg
     3001 tggtcatcag ccggacgccg gtggcgccga gcgggtggcc cagggcgatc gccccgccgt
     3061 tgacgttgac cttggacagg tccgcatcga gctccttggc ccaggccagc acgaccgagg
     3121 cgaacgcctc gttgatctcg accaggtcga tgtcgtccag cgtcatgccc gccttctcga
     3181 gcgcccgctt ggtggccggg atcggcgccg agagcatgta gacgggatcg tcggcgagca
     3241 ccgacaggtg gtggatgcgg gcccggggcg tcagaccgtg gtccttcacc gcctgctcgg
     3301 aggcgacgag catggccgcc gacgcgtcgg agatctgcga cgcgaccgcg gcggtgatgc
     3361 gcccgccctc gcgcaacgtc ttcagcgacg cgatcttcac cgggtccggt tcccgtggtc
     3421 cctcgtcgaa ggtcaacccg gcgagctcga cgatctcgtt gtcgaatcgc ccctccgccc
     3481 gggcggtgat cgcccgccgg tgggactcca tggcgaactc ctccatgtcc tcccgggaga
     3541 tctcccactt ctcggcgatc atgtcggcgc cgcggaactg gctgacctcc tggtcgccgt
     3601 agcgctcgac ccacccggtc gaaccggaga acgggtcggt gtcgccgaac tgctcggcga
     3661 cgagcatggc ggagctgatc gggatgcgcg acatgttctg caggccggcg gccacgacga
     3721 ggtcctgggt ccctgacatg acgccctgcg cggcgaagtg caccgcctgc tgcgacgacc
     3781 cgcactggcg gtcgatcgtg gtgcccggga cgtgctcggg cagaccggcg gccagccagg
     3841 cggtgcgacc gacgtcgccg gcctgggaac cgatcgtgtc gaggcagccc atgacgacgt
     3901 cgtcgacggc acccgcgtcg atgccggtgc gggccaccag ctcgcgcagc gtgtgggcgc
     3961 cgaggtccgc ggggtggatc tgcgacagcg aaccgccccg cttgccgacg ggggtgcgga
     4021 cggtgtcgat gatgtaggcc tcggccatgg gagctcctcg gagagcgacc ggtgcgggcc
     4081 ggtcggttcg gtcacgatcg gggctccaag gtacgcaacc gctgtcggtc ccaccccatc
     4141 gggcggcgac ggcccacgca ccccctccga tcgggcagac ttccgtcgca ggaagtggac
     4201 cggaccccca cgaggccggc catgacgagg ggacagccgt gaagcggacc tggcgtgccg
     4261 aggagttcga cctgctgtcg ccatcggtgg ccaagcgatg gtcggcgttc ctgaacgagg
     4321 agggcgaccg gcgctggttc ccgaacctgc tgggcatcga ggtcgaggag ctgcgccgcg
     4381 actacgcccg ccttcgcatg ccgtggcgca acgacctcaa ccaggcgacc ggcatgatgc
     4441 acggcggcgc catcgccacc ctgatcgaca ctgccgtcgt acccgcgatc ggcaccgcgt
     4501 actccgacaa ccggccgttc tccaccatcg agatgtcggt gcgctacatc caacccgtcc
     4561 gccaggagga cctggtcgcc gaggggtggg tcacccgacg gggcaagcgg gtggtgttct
     4621 gcgaggtcga ggtccgcacc gtgagcgggg tgacggtcgc caccggcgac ctgatctacg
     4681 tggtcggctc cccgcctccg cccgagtagc tcaccggcgg gcgcgttccg tccccgacca
     4741 gacccgcttt gtagtgcgag atccaggcgt tcgcctggat ctcgcactac agaacggggg
     4801 ccgcggccgg aacggggcga gggcggttgc cggtggccac cggacctgcg accattcccc
     4861 gaggcgacag cgggggttcg acatggctca gcggggacga cggcgcgggt tccgcgtcgc
     4921 gatggcgacg atcgctctcg gcagcatcgc ggcgatggcg tgcacccctc cgggtgagcc
     4981 ggcaggtccg gtcgaccccg ggccctcggt gcccagcggc gacgacgcca tcgggctcaa
     5041 ccagttccag tacctgggca gccacaactc ctacaagatc ggcccgcagg ccgacctgct
     5101 tctcatgctg gggctcggcg ccgccgcgct gcccgacctg gccggggccg gcctcgatcc
     5161 cgcccagctc aactacacgc accggccgct cacccagcag ctcgacagcg gcatccgcag
     5221 cttcgagctc gacgtgtggg ccgacccgac cggcggccgc ttcgccgcgc cgctcgccgc
     5281 gcagctgttg ggccagccct ggccgagcgg cgtcgacgcg cccggcttca aggttctgca
     5341 catccaggag atcgactacc ggtcgacgtg tccgaccctc gacggctgcc tccaggagat
     5401 gcgggcatgg tccgacgccc accccggcca cctgcccatc accatcaacc tggagctgaa
     5461 ggccgacccg ctcccggcgc cgttcgacgt cacccccatc cagccgttcg acaccccgac
     5521 gctcgacgcg ctcgacgccg acctgcgcgc ccagatcggc gaccggctga tcacccccga
     5581 cgaggtgcgc ggcaccgccc ccgatctgcg cgccgcggtg accgatcccg actcgggcgg
     5641 cggttggccg tcggtggccg acagccgggg acggttcctg ctgttcctcg acaacggcgg
     5701 gtcggtggcc gagcgctacc tcgacggtca tccctctctc gccggacggg tgctgttcac
     5761 cagcgacggg ttcgaccgcg acgacgcggc ggtgctcaag gtcaacgagc cgggcgacgg
     5821 gtctgagatc tcggcgttgg tcgcccaggg gttcctggtg cgcacccgcg ccgacgccga
     5881 tgtggtcaac ccgggcgcgg accgcactgc ggcgttggcg tcgggcgcgc agatggtgca
     5941 ctcggacttc cccgccggcg aggctcagtt cggcaccggc taccaggtct cgttcccgac
     6001 gcgggtgcag gcccgctgca accctgtgaa cacgacgtcg gccacgtgca ccgcggccgc
     6061 cgcggtcgaa ccgacctgac ccacccaccg atttcggtga ccacacgcgc caccagtggc
     6121 gcggacggtc atgaaaactc gggggcggcg gggcagatcc cgagcaggtg gcagtcggcg
     6181 caccagcggc ccgggaccgt gggggacgag tcgctgtcga cccgccaccc caccagttcg
     6241 acgacgcggt cgatgaccgg ctcgagcagg tccatgctga cggggaacgc tgtggtcgaa
     6301 gcggtcgccg ccgacgcgac ccggacccgg tcgggaaccc gactccgcaa caggccgtcg
     6361 accagagcgt tgaagccggc cagccgggcc gccgccggat cggcgggtgg tcgggtcgag
     6421 atcacgacga gcacctcggg acgggccccg ctcgacgacg cgtcccagct ggtcctcagc
     6481 cggagcgttc gaccgtcgac gtcatggggc tggcgggcag tggcccagtt gagcgaccgc
     6541 cccccgaccg cggccagcgc gccgtgagcc caggtgatcg ccgcggcgcg cagggcggcc
     6601 tgcccggagc ggtccagctc ctggtcgtac caggacgcga accacgccac cgagggatcg
     6661 gcgtcggcca ggccggctgt cacgcgggcg accgcgacgc cgaccggctc accctcacgc
     6721 cgctcgcgga gtgccagccg accgaccgcc cccgccgcgg tgcggaccga gagaacgaag
     6781 tcgtcgaagg ggaccgacca gcgggccgga cagcgcagct cgaggtcgtg ggctgacacg
     6841 tcgacccagc cggcgccgtc gcggggtcgg ggtccgtcac ggcggagccg ctcacccagc
     6901 cgggcgtcga gtcgctcgtg caggagggat cgcgcccgag ctgccagatc gtctcgatcc
     6961 cgatcgctca cgcgtcgact ccgaacgggt cgggcgcggg agcgaagctg gggcacaccc
     7021 cggcgtcggg acaccacgaa caggccggac ccggtcgttc ccggggcggg cgtccgaccg
     7081 cgagctcggc ccaggcgtcg atcgcggcgc cgaggcgacg ggcggcggac tcgagcacgt
     7141 cggaggtgac cgccatggtg gccagcgcac cgcccgggta ccagcgggcg accgccgcgg
     7201 gtgcacggcc gtcgcggagc gcggtcagca gcgcgtagtg ggtggcct
//
LOCUS       DAOQOD010000159         7244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_3908_length_7244_cov_11.878704, whole genome shotgun sequence.
ACCESSION   DAOQOD010000159 DAOQOD010000000
VERSION     DAOQOD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_7244_cov_11.878704"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..820
                     /locus_tag="PLZ72_12995"
     CDS             95..820
                     /locus_tag="PLZ72_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37261.1"
                     /translation="MSPRKSRQPEISEPPALEVVGLSKFYGDVAALEETDLEVPFGQS
                     VVLVGHNGSGKSTLLSMAAGVLEPTGGEVLVHGEHNGTLSARAELSWLPDNPVLYDDL
                     SLWEHVEFINRMHGGTGDDPFLDELLERLGVDDRRDHLPSQFSRGLRQKSAIAVALCR
                     PFSVLLVDEPFVGLDASGRLALLELLDEARANGATVVVATHDPDVIDRFERGIMLENG
                     ELVHDGPARELHDLLGADDRRAG"
     gene            complement(835..1758)
                     /locus_tag="PLZ72_13000"
     CDS             complement(835..1758)
                     /locus_tag="PLZ72_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA37262.1"
                     /translation="MRFSVWPNLMQPVEDVLDVARHADRTGWDGVWVADHFMGDGAGF
                     GAVETPTLESTAVLSALAVATERVRIGPLVLGATYRHPAVVANWAITTDRLSSGRLVL
                     GLGAGWQRNEHDQYGIDLPAVGDRVTRFSEYVRVVRRLFEEPTADFDGQWFRLSGARC
                     EPKAEPGPGRPGGIPILVGGKGDRMIALAARQADEWNMWGLPDLIAERSAVLDRGCEA
                     IGRDPASVGRSTQALLLPTEDDARARRFTDAVAPRAAVAGSADRIADTVARWAEVGVD
                     EVIVPDSPLGRGSERRDHLDALAEVFAPFRG"
     gene            complement(1771..3015)
                     /locus_tag="PLZ72_13005"
     CDS             complement(1771..3015)
                     /locus_tag="PLZ72_13005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNA37263.1"
                     /translation="MTSSPNLAIRLSPNALKHVRQGHPWVFDGGIESVRGGRYGDGTG
                     EPGDVAVIFDDRRRFVAVGLWDPRSPIRIKVLHAASPVTVGADLWHDRLAHALAHRWE
                     LLGSADTDALRIVHGENDRLPGLVLDLYRTVAVLKLYSAAWVPHLDEVLGVIRSTIWS
                     IDGIVLRLARSVAADPTVREADLADGAVLHGVVPDRLVEFREHGLRFTADVRRGQKTG
                     WFLDQRDNRDRVHALADGATVLDVFCCGGGFSVHAAAGGARHVHSVDRSPHAIDDTVR
                     HLSMNRDIPGVVAVDARQTVGDAFEVMDDLRRAGDRYDLVIVDPPSFASRRSDVRGAL
                     QAYAALTTRAVELVVPGGTLVQASCSSRVHPDDFTTIVEKAAGRAGVHLEVFDRTAQP
                     IDHPVGFPEGRYLKAVYARVNP"
     gene            complement(3041..4441)
                     /locus_tag="PLZ72_13010"
     CDS             complement(3041..4441)
                     /locus_tag="PLZ72_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA37264.1"
                     /translation="MSSDFPYAERFAVNRTFPAHGRDREDIIDELSQMAKEEDAFWET
                     GQVSGTMYCGDHSHYDFLTRAFGLFAHVNVLQRDMCPSATRFEGEIIAMALDLFHADA
                     IDDGDPVGLVTSGGTGSISHAVLAYRDHARATHGITRPNFVKPETGHPAFDKACHLFG
                     VDLRVAPVDPATTRVDVAAMAELIDDDTIAILGSAGNYPYGTIDDIEALSDLALSRGV
                     GLHVDGCLGGFILPFGEELGVENIPVFDFRLPGVTTISADTHKYGYSVKGTSVCLFRD
                     RAVRNSQYFFRTDWSGGKYCSPGMDGSRSGGLLAATWASMVSLGREGYRDYARQIFDT
                     AEQMRQTVSSHPELRLMGQPTFVLSFTSDEFDIYLVNDFMRLRGWRFNGQQYPNAIHI
                     AVTRPQTLPGVTDRFAADLAEAVVYAREATERGEAAASGAIYGGVAGGMTDEADEFIR
                     MIMSDMMDTQQSLPPS"
     gene            4507..4860
                     /locus_tag="PLZ72_13015"
     CDS             4507..4860
                     /locus_tag="PLZ72_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNA37265.1"
                     /translation="MDDPAPRPDIDPERFFEVDLRVGRISDVRPFPEARRPAYQLVVD
                     FGPAVGTLETSAQVTNYRPEELLGRSVVGAINLGVRRVAGFRSQFLVLGAVETDGAVR
                     LLRPDGDPAPGTPIA"
     gene            complement(4921..5403)
                     /locus_tag="PLZ72_13020"
     CDS             complement(4921..5403)
                     /locus_tag="PLZ72_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNA37266.1"
                     /translation="MDRDEVRNFIERSRTCTLATLNADGSPHLVAMWYAVIDGQVWFE
                     TKAASQKAVNLRRDPRVTILIEDGLTYDKLRGVSLEGTAEVVEDPDTLWKVGISVWER
                     YNGPYTEEMRPLVEFMLHKRIAVRVDASRIRSWDHRKLGMDPLELGGSTAEHLDDQLL
                     "
     gene            complement(5542..6747)
                     /locus_tag="PLZ72_13025"
     CDS             complement(5542..6747)
                     /locus_tag="PLZ72_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA37267.1"
                     /translation="MPAASDPSAALGVPAVGEHRRSLLSPGFVLVTAATLFYFIALGA
                     LLPTLPVYVEDELGGGSVAVGVTVGVFAVSAALLRPLAGRLGDTRGRRILVIGGSLVM
                     GVSVLAYTLVDSIAALVALRLLTGAGEAAMWVGAATAIQDMAPDDRRGEAASYFSVAL
                     YAGLAFGPLVGEGLRDSAGFHAVWIFAGCCGLVACALGFGTPRDVRREPQPFRLLHPA
                     ALGPGAILLLGMLPFIGFATFIALYGPTVGFADVAPLLLVYGVLVLVIRVVGAKLPDQ
                     LGWTRASSTALSVLAVGGLLLGLWSGTAGVWLAVVALAIGMSLLFPALFSATVAAVPE
                     SERGQAVGTFSLSFDLASGLGPAVLGVVVALADYRAAFVVAGIGAAAGLALVGPVARR
                     AAASARSTG"
     gene            6919..>7244
                     /locus_tag="PLZ72_13030"
     CDS             6919..>7244
                     /locus_tag="PLZ72_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37268.1"
                     /translation="MSDGRRVVVLGRGFVGRAVDRRLRADPGHEVVVLEPADDAELAG
                     RTPAGAERLRAAIGSSSKPVVINCCGRLRGTDEELLDANARWPQWLTEVLDGAAVRLV
                     HLGSAS"
BASE COUNT         1086 a         2515 c         2623 g         1020 t
ORIGIN      
        1 gaccgccacc ggaggtggcg ccgcgtgacc gccaccggag gtggtgccga atgactgccc
       61 gttcgatccc ggcgcccggt cagagaggtg agaggtgagt cccaggaaga gccgacagcc
      121 cgagatctcc gagccgcccg cgctcgaggt ggtggggttg tcgaagttct acggcgacgt
      181 cgccgccctc gaggagaccg acctcgaggt gccgttcgga cagtcggtgg tgctggtcgg
      241 ccacaacggc tccggcaagt ccacgttgct gtccatggcg gcgggcgtcc tggagccgac
      301 cggcggcgag gtcctggtgc acggcgagca caacgggacc ctctcggcgc gcgccgagct
      361 gtcgtggctg cccgacaacc ccgtcctcta cgacgacctg agcctctggg agcacgtcga
      421 gttcatcaac cggatgcacg gcggtaccgg tgacgatccc ttcctggacg agctgctcga
      481 acggctgggg gtcgatgatc gccgcgatca ccttccttcg cagttcagcc gggggttgcg
      541 ccagaagtcg gcgatcgcgg tcgccctgtg ccgtccgttc tcggtgctgc tggtcgacga
      601 gccgttcgtc ggtctcgacg cgtccggacg gctggccctg ctcgagctgc tcgacgaggc
      661 ccgcgccaac ggcgcgaccg tcgtggtcgc gacgcacgac cccgacgtga tcgaccgctt
      721 cgagcggggg atcatgctcg agaacggcga gctggtccac gacggccccg ctcgggagct
      781 gcacgacctg ctcggagccg acgaccgccg cgccggctga cgccggtccg ggcgtcagcc
      841 ccggaacgga gcgaagacct cggccagtgc gtcgaggtga tccctgcgtt cggatcctcg
      901 accgagtggg gagtcgggga cgatcacctc gtccacgccg acctcggccc agcgggccac
      961 cgtgtccgcg atccgatcgg ccgagcccgc caccgcggcg cggggcgcga cagcatcggt
     1021 gaaccggcgg gcgcgtgcgt cgtcctctgt cggcagcagg agtgcctgcg tgctgcgacc
     1081 caccgacgcc gggtcccgcc cgatcgcctc gcagccccgg tcgaggaccg ctgaacgctc
     1141 ggcgatcagg tcgggcaggc cccacatgtt ccactcgtcg gcctgacggg cggccagggc
     1201 gatcatgcgg tcgcccttgc cgccgaccag gatcgggatc ccgcccggac gcccgggccc
     1261 gggttccgcc ttgggttcgc acctggctcc gctgagacgg aaccactgcc cgtcgaagtc
     1321 agcggtcggc tcctcgaaca gacgacgtac cacccgcacg tactccgaga accgggtcac
     1381 ccggtccccc actgcgggca ggtcgatgcc gtactggtcg tgctcgttgc gctgccagcc
     1441 cgctccgagt ccgagcacca gccgtcccga gctcagccgg tccgtcgtga tcgcccagtt
     1501 ggcgaccact gccgggtgcc ggtaggtggc gccgaggacc agcgggccga tccggacccg
     1561 ctcggtggcc accgcgaggg ccgacaggac cgccgtcgac tcgagggtcg gggtctccac
     1621 cgcgccgaag ccggcaccgt cgcccatgaa gtggtccgcg acccagaccc cgtcccaccc
     1681 ggtgcgatcg gcgtgccgcg cgacgtcgag gacgtcctcg accggttgca tcaggttggg
     1741 ccacacggag aatcgcatgt cgggatcctg tcaggggttc acacgggcgt agaccgcctt
     1801 gaggtaccgg ccctcgggga agccgacggg gtggtcgatc ggctgcgcgg tccggtcgaa
     1861 cacctcgagg tggacgccgg cgcgaccggc ggccttctcg acgatggtcg tgaagtcgtc
     1921 ggggtggacc cggctggagc acgatgcctg gaccagggtg cctcccggca ccaccaactc
     1981 gacggcccgt gtggtcaacg ccgcgtacgc ctggagcgct ccccggacgt cggaacgccg
     2041 cgacgcgaag gaaggcgggt cgacgatcac caggtcgtag cggtcgccgg cgcggcggag
     2101 gtcgtccatg acctcgaagg cgtcgcctac cgtctgtcgg gcgtcgaccg ctacgacccc
     2161 ggggatgtca cggttcatcg acaggtgccg gacggtgtcg tcgatggcgt gggggctccg
     2221 gtccacgctg tggacgtgac gggcgccgcc ggccgccgcg tggacgctga acccacctcc
     2281 gcagcagaag acgtcgagca cggtggcccc atccgcgaga gcgtggaccc gatcacggtt
     2341 gtcgcgctgg tccaggaacc agcccgtctt ctgaccgcga cggacgtcag ctgtgaaccg
     2401 caggccgtgc tcgcggaact ccacgaggcg atcgggcacc acgccgtgga gcacggcgcc
     2461 gtcggcgagg tcagcttccc gcaccgtggg atccgcggcg accgatcggg ccaggcggag
     2521 cacgatcccg tcgatcgacc agatcgtgga gcggatcacc ccgaggacct cgtccaggtg
     2581 gggaacccag gccgccgagt acagcttgag caccgcgacc gtgcggtaca ggtcgagcac
     2641 gagcccgggc aggcggtcgt tctcgccgtg gacgatccgc agcgcatcgg tgtcggcgga
     2701 tccgagcagc tcccatcgat gcgcgagcgc gtgcgcgagc cggtcgtgcc agaggtcggc
     2761 gccgacggtc accggcgagg cggcgtgcag caccttgatg cggatcggtg aacgcgggtc
     2821 ccacagcccg acggcgacga accgtcggcg gtcgtcgaag atgaccgcca cgtcccccgg
     2881 ttcgccggtg ccgtcgccgt atcgtccgcc ccgaaccgac tcgatgccgc cgtcgaagac
     2941 ccacgggtgt ccctgacgga cgtgcttgag cgcgttgggg ctcagccgga tcgccaggtt
     3001 gggcgagctg gtcaacgggg tcggtcccgg acagcggaca tcagctcggc ggaaggctct
     3061 gctgggtgtc catcatgtcg ctcatgatca tccggatgaa ctcgtcggcc tcgtcggtca
     3121 tgccgccggc cacgccgccg tagatcgcac cgctcgccgc tgcctcgccc cgctccgtgg
     3181 cctcgcgggc gtagacgacg gcctcggcca ggtcggcggc gaaccggtcg gtgacccccg
     3241 gcagcgtctg gggtcgggtg acggcgatgt ggatggcgtt ggggtactgc tggccgttga
     3301 accgccagcc ccggagacgc atgaagtcgt tgaccaggta gatgtcgaac tcgtcggagg
     3361 tgaagctcag cacgaaggtg ggttggccca tcaggcgcag ctcggggtgg ctcgacaccg
     3421 tctggcgcat ctgctcggcg gtgtcgaaga tctgtcgggc gtagtcgcgg tagccctcgc
     3481 gtccgagcga caccatcgac gcccatgtcg cggccagcag cccgcccgag cgcgacccgt
     3541 ccatgcccgg cgagcagtac ttgccgccgc tccagtcggt ccggaagaag tactggctgt
     3601 tgcgcacggc gcggtcccgg aacaggcaca ccgaggtgcc cttcaccgag tagccgtact
     3661 tgtgggtgtc ggcggagatg gtggtcaccc ccgggaggcg gaagtcgaac accgggatgt
     3721 tctccacccc gagctcttcg ccgaagggga ggatgaagcc gcccaggcag ccgtcgacgt
     3781 gaagcccgac gccgcgcgac agcgccaggt cgctcagcgc ctcgatgtcg tcgatcgtgc
     3841 cgtacgggta gttgccggcg gagccgagga tggcgatcgt gtcgtcgtcg atcagctcgg
     3901 ccatcgccgc gacgtccacc cgggtcgtcg ccgggtcgac cggggcgact cgcagatcga
     3961 ccccgaacag gtggcacgcc ttgtcgaacg cggggtggcc ggtctcgggc ttgacgaagt
     4021 tgggtctggt gatcccgtgg gtggcgcggg cgtggtcgcg gtaggccagc acggcatggg
     4081 agatggagcc cgtgcctccg ctcgtgacca ggccgacggg gtcgccgtcg tcgatggcgt
     4141 ccgcgtggaa caggtcgagg gccatggcga tgatctcgcc ctcgaagcgc gtcgcgctcg
     4201 ggcacatgtc gcgctgcagg acgttcacgt gggcgaacag cccgaaggcg cgggtgagga
     4261 agtcgtagtg gctgtggtcg ccgcagtaca tggtccccga gacctgcccg gtctcccaga
     4321 aggcgtcctc ctccttggcc atctggctga gctcgtcgat gatgtcctcg cggtcgcggc
     4381 cgtgcgccgg gaaggtccgg ttgacggcga agcgctcggc gtaggggaag tcgctgctca
     4441 tcgtgctcct cggagggcgg tccggatgct gctccggagt gcgaccagcg tgccacactc
     4501 cccgaggtgg acgacccggc accccgaccc gacatcgacc ccgagcggtt cttcgaggtc
     4561 gatctccgcg tcggccgcat cagcgacgtg cgccccttcc ccgaggcgcg tcgtcccgcc
     4621 taccagctcg tggtcgactt cggcccggcg gtcgggacgc tggagacctc ggcgcaggtc
     4681 acgaactacc gcccggagga gctgctggga cggtcggtgg tcggagcgat caacctgggt
     4741 gtgcggcgcg tcgccgggtt ccgcagccag ttcctggtgc tcggcgcggt cgagaccgat
     4801 ggagcggttc ggttgttgcg acccgatggc gacccggcgc cgggcacccc gatcgcctga
     4861 ccgccgccga acttgtagct ggccggtcac ccagcggcgc gtgggctaca agttcgccgg
     4921 tcagagcagc tggtcgtcga ggtgctccgc cgtgctgccg ccgagctcga gggggtccat
     4981 cccgagcttg cggtggtccc acgagcggat ccggctggcg tcgacccgta ccgcgatccg
     5041 cttgtggagc atgaactcga ccagcggtcg catctcctcg gtgtaggggc cgttgtatcg
     5101 ctcccagacg ctgatgccga ccttccagag tgtgtcggga tcctcgacga cctcggcggt
     5161 cccctccaac gacacgcccc ggagcttgtc gtaggtcagg ccgtcctcga tcaggatcgt
     5221 gacccgaggg tcgcgtcgga ggttcaccgc cttctgggat gccgccttgg tctcgaacca
     5281 gacctggccg tcgatgaccg cgtaccacat ggcgacgagg tggggagatc cgtccgcgtt
     5341 caacgtcgcc agcgtgcagg tccggctccg ctcgatgaag ttgcggacct cgtcacggtc
     5401 catccccacc ttgctgcgct cgttcgtacc cacgacgacc cccgttgaga tgcccggttc
     5461 cggaaacccg gggtggtcac atcatgcccg cccgtcgagg gtcgtcggaa tcgtcagagc
     5521 ggagcgacgc aggctgtccg gtcagcctgt cgagcgtgcc gatgcagcag cgcgacgggc
     5581 gaccgggccc accaatgcga gtccggcagc ggctccgatc ccggccacga cgaaggcggc
     5641 ccggtagtcg gcgagcgcca ccaccacgcc gagaaccgcg ggtccgagcc cgctcgccag
     5701 gtcgaacgac agcgagaacg tgcccacggc ctgacctcgc tcggattcgg gtaccgcggc
     5761 gaccgtggcg ctgaacaggg ccgggaagag gagcgacatg ccgatcgcca gggcgacgac
     5821 ggccagccag acccccgccg tccccgacca gagacccagc agcaggccgc ccacggccag
     5881 gaccgacaac gcggtcgacg acgcccgggt ccatccgagc tggtcgggca gcttggcccc
     5941 gacgacacgg atgaccagga cgagcacgcc gtagaccagc agcaggggcg cgacgtcggc
     6001 gaagccgacc gtcgggccgt acagcgcgat gaacgtggcg aacccgatga acggcagcat
     6061 cccgaggagg aggatcgcac cgggcccgag cgccgccggg tgcaggaggc ggaacggctg
     6121 cggctcccgt cggacatcgc gaggggtccc gaagccgaga gcgcacgcga ccagtccgca
     6181 gcaaccggcg aagatccaca cggcgtggaa cccggccgag tcgcggaggc cctcgccgac
     6241 gagcggcccg aacgccaggc cggcgtacag cgcgaccgag aagtacgacg ccgcctcgcc
     6301 gcgacggtcg tcgggagcca tgtcctggat ggcggtggcg gcgccgaccc acatcgcggc
     6361 ttcgcccgct ccggtcagga gtcgcagcgc aaccagagcc gcgatgctgt cgacgagggt
     6421 gtaggcgagg accgacactc ccatgaccaa cgagccgccg atgaccagga tgcgtcggcc
     6481 tcgggtgtcg cccagccgcc ccgccagcgg gcgcagcagt gcggcggaga cggcgaaaac
     6541 cccgacggtg acgcccacgg ccacgctccc acctccgagc tcgtcctcaa cgtacaccgg
     6601 cagggtgggg agcagggcgc cgagggcgat gaagtagaag agcgtcgccg cggtgaccag
     6661 gacgaagccg gggctcagca gggaccggcg gtgctcgccc acggcgggga cgccgagcgc
     6721 ggcggagggg tcggaggcgg cgggcacgac ccgacgctat cgcccgggtc cttcggatgc
     6781 cgaacaactt gccggcgggg aacttctcgg tcggatcgcg ccgcgtcggt acactccacg
     6841 gcgaggagcg agcgcgttcg tgcgcccggg aagctgccgg ttcagccggc tcggagggag
     6901 agcaatgggg aaccggcggt gagcgacggt cgtcgtgtcg tcgtcctcgg ccgcggattc
     6961 gtgggccgcg ccgtcgatcg acggctgcgg gccgaccccg gccacgaggt cgtggtgctc
     7021 gagccggccg atgacgcgga gctcgccgga cggaccccgg caggagcgga gcgccttcgg
     7081 gccgcgatcg gctcgtcgtc caagccggtc gtgatcaact gctgcggccg gctgcggggc
     7141 accgacgagg agctcctcga cgccaacgcc cgttggccgc aatggctgac cgaggtcctc
     7201 gacggcgccg cggtccggct ggtccacctc ggctcggcgt ccga
//
LOCUS       DAOQOD010000160         7123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4013_length_7123_cov_11.367855, whole genome shotgun sequence.
ACCESSION   DAOQOD010000160 DAOQOD010000000
VERSION     DAOQOD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_7123_cov_11.367855"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..844)
                     /locus_tag="PLZ72_13035"
     CDS             complement(14..844)
                     /locus_tag="PLZ72_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNA37269.1"
                     /translation="MTLSLYVVAALTLISSLIGSVVGIGGATLLVPVLLALGVSPVDA
                     APIGLISVGASSLAAGARQLDEGRVHHRIGLTFELAASAGAAAGALVSSSVGEDVMAL
                     VLAVAALVGAVAAVARKGVRNLPSGTFGAEEVPGEWPGTLGGQYVLDGRVVPYHAERV
                     PAGLVAALAAGVVSGLSGVGAGFLKSPAMSEIMRIPVKVAAATTTFTLGITATTGLLV
                     YLLRGDLDPEVGSAALAGALIGGILGARLQTGASPLLLRRITAAILVAIAVLLIGRAL
                     "
     gene            complement(841..2403)
                     /locus_tag="PLZ72_13040"
     CDS             complement(841..2403)
                     /locus_tag="PLZ72_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNA37270.1"
                     /translation="MPAPTTTEVPWWRGGVVYQVYPRSFADSNGDGVGDLVGAASRLG
                     HLRDLGVDAVWFSPFYRSPMADFGYDIDDHCDVDPLFGDLGAADRLIETAHRSGLRVV
                     LDFVPGHTSDRHPWFGESRSSRGNPRRDWYVWRDPAPDGGPPTDWRAAFGDYPAWTLD
                     PTTGQYYLHLFLPEQPDLDWNHPEVAAAMIDVLRFWSDRGVDGFRIDVVHTVGKRLDV
                     DTPAELRGIPACVLDHGPGVHLRLTELRSRTDAFPSPPALIGETYVFDRERVVDFLGH
                     GDQLHLAFNIPALHSPWDAAVWRDEMASAIDLYDPVDGWPCWVLSNHDVPRHRTRYGT
                     EARARAAAVLLLTQRGTPFLYQGEELGLADAVVEGDRVVDPGGRDGCRAPIPWDDSPG
                     CGWPADPWLPFAPDAAALSVARQSGDPASMLEHYRRLLRLRRTEPVLSVGRVEILDAP
                     DGVLRYRRWLDEPPGGAAAELEVLVNFTDEPVEVPGVSGRWVGGTARPGETPGPGQGL
                     GPDEARILDPRG"
     gene            2498..2665
                     /locus_tag="PLZ72_13045"
     CDS             2498..2665
                     /locus_tag="PLZ72_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37271.1"
                     /translation="MKKGRHRRYEEARAMKRGPDLDIEAIFTDLESDRPPLVDDRDSS
                     YDDDDPYDDRD"
     gene            complement(2647..3594)
                     /locus_tag="PLZ72_13050"
     CDS             complement(2647..3594)
                     /locus_tag="PLZ72_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37272.1"
                     /translation="MEPTGGSGATAELWKRRSWRSPAVASAAVLTGIAVSDLTDATAL
                     ALAASDEARALLDGMELRIRTLPTTVATSSERCVNSVRGPIMWAETITARANALGNDD
                     VFVCMTTARSFDTVENQLLVEALDSIAAASRALRGPTAERLEADDAERIAEVAAEAAR
                     WRAEDRLAGVKPHRLTGRAAARLRGGHRRTRMAPIQAVRARAREPFCAEDVQGLADDW
                     TEVLHRTVVRVLDAVGGPTVLTLSDGGLWCRALSFRHPASSGSAPAGLALRGRPILPP
                     RGAVDGAPWADELPSDGIRLTEDLGPDEIRKVLAQSRSS"
     gene            complement(3596..4480)
                     /locus_tag="PLZ72_13055"
     CDS             complement(3596..4480)
                     /locus_tag="PLZ72_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37273.1"
                     /translation="MIFPPYVVDQLVAALDAGKHVILTGPPGTGKTTLAYLAAELGRH
                     SMLCTGYQPTTATSEWTTFETIGGLQPTPEGLIFRPGLFVEAIETGKWLVIDELNRSN
                     FDRAFGQLFTVLSGSAVVLPFRRSGQIKPISLVPHGVEPPGETDPIRLPASWRIIATM
                     NALDKHLLFDMSYALMRRFAFIEVTTPPDWAYEKLLDGDGEIVKKLLPLRQLNNLGPA
                     IYVDAQRYVARRSRDDISESRLLFELFYAFFLPQFEGIDEHTAVALYRTVAKPLDPPE
                     QQEARRVIAELLGEELVG"
     gene            complement(4584..5348)
                     /locus_tag="PLZ72_13060"
     CDS             complement(4584..5348)
                     /locus_tag="PLZ72_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37274.1"
                     /translation="MSERRDRDKTHSEAHPRQWVSFEHDDDTWMFDATFLMSPYRCIF
                     NDGCKGVLEEDAEHLGHGCCSFGAHFADKADRVRVRKLIDRLDRDQWQFRREARKHGG
                     AIGRNDDGDWVTRVHDGACILLNRPDFHRGAGCALHVAALDAGERPMDWKPEVCWQVP
                     LRLEYHTDEVGHTTHTLREWRRRDWGEGGQEFHWWCTEESEAFTAHEPLVTTCRDDIV
                     GLVGEERYELLRAALGVDDGPAAGVTWLPHPAVRRR"
     gene            5411..5608
                     /locus_tag="PLZ72_13065"
     CDS             5411..5608
                     /locus_tag="PLZ72_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37275.1"
                     /translation="MAGEFDDIRTRLEAISEELADLAILRLRESIDAGGDELPVDERR
                     LTRARRAVDKAAHLLAESDGA"
     gene            5636..6208
                     /locus_tag="PLZ72_13070"
     CDS             5636..6208
                     /locus_tag="PLZ72_13070"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNA37276.1"
                     /translation="MGTVHDGIDDGLRDWIERQHVVFVATAPSDPSGLVNLSPKGMVD
                     TFTVVDRHRCAYLDLTGSGAETVAHLRDNGRITAMWCAFDGPPRIVRCHGTGRVVTPD
                     DAEFESWRERFGPHRGARAVVVIDVIRVADSCGYAVPLMEFVGERDRLVRWTESRTDE
                     ELEQYRRDRNAVSIDGLPAFDKWILGEFKG"
     gene            6307..7095
                     /locus_tag="PLZ72_13075"
     CDS             6307..7095
                     /locus_tag="PLZ72_13075"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNA37277.1"
                     /translation="MPARPLTAPEREEIRAGIERREPDRVIAGRLGRHRATINAEINR
                     NGGRAGVAATAAQTRADACRACPKDSKLEADPVLAAHVSARLEAKDSPMTISIELARG
                     VHGFEAEISDECIYQAVYAHGRRGLRAGLHEGLHRRRRCRKHRVIGPPAPKTSPLGTF
                     NLIGLRPAVAAERVEVGHREGDLIVGSFNRSAIATVFDRASRHLWLANFPEDHGADAT
                     LAALCEILDRIPAPLRTLTWDQGRPTCQYECRSVEWPYSMRAGR"
BASE COUNT         1090 a         2527 c         2534 g          972 t
ORIGIN      
        1 gggtcggtgc cggtcacagc gcccgtccga tcaggagcac ggcgatggcg accaggatcg
       61 ccgcggtgat gcgccgcagc agcagcggcg aagccccggt ctggaggcgg gcgccgagga
      121 tcccaccgat cagcgcgccg gccagggccg cgctgccgac ctcggggtcg aggtcgccgc
      181 gcaggaggta gacgaggagt ccggtcgtcg ccgtgatgcc gagggtgaag gtggtggtcg
      241 ccgccgccac cttgaccggg atccgcatga tctcgctcat cgccggcgac ttgaggaacc
      301 cggcgcccac cccggacagg cccgacacga ccccggccgc cagtgccgcc accagaccgg
      361 ctggcacccg ctcggcgtgg tagggcacga cccggccgtc gagcacgtac tggcctccca
      421 gcgtgcccgg ccactcaccg gggacctctt cggccccgaa cgtgccgctg gggaggttgc
      481 ggaccccctt gcgggccacg gccgcaaccg caccgacgag ggcggcgacg gccagcacca
      541 gcgccatgac gtcctcgccg acgctcgagg agaccagcgc tccggcggcc gctcccgctg
      601 atgcggccag ctcgaaggtc aagccgatcc ggtgatgcac ccgaccctcg tcgagctggc
      661 gggccccggc cgccagcgag gacgctccga ccgagatgag cccgatcggg gcggcgtcga
      721 cgggggacac gccgagggcg agcagcaccg ggacgagcag ggtcgctccg ccgatgccca
      781 cgaccgagcc gatcagcgag ctgatcagcg tgagcgcagc gacgacgtag aggctgagcg
      841 tcaccctcgg gggtccagaa tgcgcgcttc gtcggggccc agcccctggc ccggaccggg
      901 ggtctcgccg ggccgggcgg tgccgccgac ccatcgaccg gacaccccgg gtacctcgac
      961 gggctcgtcg gtgaagttga cgaggacctc gagttcggcg gcggcccctc ccggaggttc
     1021 gtcgagccac cgccggtagc ggagcacccc gtcgggggcg tcgaggatct ccacgcgacc
     1081 gacgctcaga accggctcgg tccgccgcag gcgcagcagc cgccggtagt gctccagcat
     1141 cgaggcggga tcgccgctct ggcgagcaac gctgagcgcg gcggcgtcgg gagcgaacgg
     1201 cagccacggg tccgcgggcc agccgcagcc gggggagtcg tcccacggga tcggggcccg
     1261 gcacccgtcc cggccgcccg ggtcgaccac ccggtcgccc tccaccaccg cgtcggcgag
     1321 gcccagctcc tcgccctggt acaggaacgg ggttccccgc tgggtgagca gcagcaccgc
     1381 cgcggcgcgg gcccgggcct cggtgccgta gcgggtgcga tgccgtggca cgtcgtggtt
     1441 cgacagcacc cagcacggcc atccgtcgac cgggtcgtac aggtcgatcg cgctcgccat
     1501 ctcgtcgcgc cacaccgcgg catcccacgg cgagtgcagc gccgggatgt tgaacgccag
     1561 gtgcagctgg tcgccgtggc ccaggaagtc gacgacccgc tcgcgatcga acacgtaggt
     1621 ctcaccgatc agcgccggcg gcgacgggaa cgcatcggtc cgcgaccgca gctcggtgag
     1681 gcgcaggtgc accccgggac cgtggtcgag cacgcaggca gggatccccc ggagctcggc
     1741 gggcgtgtcg acgtcgagcc gcttgccgac ggtgtgcacg acgtcgatgc ggaagccgtc
     1801 gacgccacga tcgctccaga agcgcaggac gtcgatcatc gccgccgcga cctcggggtg
     1861 gttccagtcc aggtcgggct gttcgggcag gaagaggtgc aggtagtact gaccggtggt
     1921 cggatccagc gtccaggccg ggtagtcgcc gaacgcggcg cgccagtcgg tcggcggacc
     1981 accgtcggga gccgggtcgc gccacacgta ccagtcgcga cgggggttcc cgcgggacga
     2041 gcgggactcg ccgaaccacg ggtgacggtc cgaggtgtgc ccgggaacga agtccaacac
     2101 gacccgcagg cccgagcggt gagcggtctc gatcagccga tcggccgcgc cgaggtcccc
     2161 gaagagcggg tcgacgtcgc agtggtcgtc gatgtcgtag ccgaagtcgg ccatggggga
     2221 gcggtagaac ggcgagaacc acaccgcgtc cacgcccagg tccctcaggt ggcccaggcg
     2281 ggaggccgcg ccgaccaggt cgccgacgcc gtcgccgttc gagtcagcga agctccgtgg
     2341 gtacacctgg tagacgactc cgccccgcca ccacgggact tccgtcgtgg tcggggcagg
     2401 catcgacaca ccctatcgac cggtcggggg tgtgaccggt ccggacgacc gccgcgctac
     2461 actgacggct ccggtcgttg atgacgggga gtgctctgtg aagaagggtc ggcatcgccg
     2521 ctacgaagag gcccgcgcca tgaagcgcgg cccggacctc gacatcgagg ccatcttcac
     2581 cgatctcgaa tcggatcgac ccccgctcgt ggacgaccgc gactcgtcct acgacgacga
     2641 cgatccctac gacgaccggg actgagccag gaccttccgg atctcatccg gtccgaggtc
     2701 ctctgtcagc cggatcccgt cgctcggcag ctcgtccgcc cacggagctc cgtccaccgc
     2761 gccccgaggt ggcaggatcg gccgcccgcg caacgccaga ccggccggag cggaccccga
     2821 cgacgcgggg tgccggaacg agagcgcccg acaccacagg ccgccgtcgc tcagcgtgag
     2881 caccgtcggg ccgcccaccg cgtcgagcac ccgcacgacg gtgcggtgca ggacctcggt
     2941 ccagtcgtcg gcgagcccct ggacgtcctc ggcgcagaac ggctcccggg cccgcgcccg
     3001 aaccgcctgg atcggagcca tccgggtccg gcggtgaccg ccgcgcagcc gtgccgccgc
     3061 ccgtccggtc aaccggtggg gcttcacgcc ggccagccgg tcctcggccc tccaacgggc
     3121 cgcctccgct gccacttcgg cgatccgctc ggcgtcgtcg gcctcgagtc tctcggcggt
     3181 cggcccgcgc agcgcccggg atgccgcggc gatcgagtcc agcgcctcga ccagcagctg
     3241 gttctcgacc gtgtcgaagc tgcgggcggt cgtcatgcag acgaacacgt cgtcgttgcc
     3301 cagagcgttg gcccgggcgg tgatcgtctc tgcccacatg atcggtcccc gcacggagtt
     3361 gacgcagcgc tccgacgacg tggcgaccgt cgtcggcagc gtgcggatgc ggagctccat
     3421 cccgtcgagc agggcgcggg cctcgtcgga cgccgccagc gccagggcgg tcgcatcggt
     3481 gaggtcggac acggcgatcc cggtcaggac cgcggcgctg gccacggcgg gggagcgcca
     3541 ggaacggcgc ttccagagct cggcggtggc gccggagccc ccggtgggct ccacgtcagc
     3601 cgaccagttc ctcgccgagc agctcggcga tcacccgtcg ggcctcctgc tgctcgggcg
     3661 gatcgagcgg tttggccacg gtgcggtaca gcgccactgc ggtgtgctca tcgatcccct
     3721 cgaactgggg caggaagaac gcgtagaaca gctcgaacag gagtcgggac tcgctgatgt
     3781 cgtcgcggct gcgacgggcg acgtagcgct gggcatcgac gtagatcgcc gggccgaggt
     3841 tgttgagctg gcgcagcggc agcagcttct tgacgatctc accgtcgcca tccagcagct
     3901 tctcgtaggc ccagtccggc ggggtggtca cctcgatgaa cgcgaagcgg cgcatcaggg
     3961 cgtaggacat gtcgaacagc aggtgcttgt cgagcgcgtt catggtggcg atgatccgcc
     4021 agctggccgg cagtcggatc gggtcggtct caccgggcgg ctccaccccg tgcggcacga
     4081 gcgagatcgg cttgatctga ccgctgcgcc ggaacggcag cacgacagcc gagcccgaca
     4141 ggacggtgaa gagctggccg aacgcccggt cgaagttcga ccggttcaac tcatcgatga
     4201 ccagccattt gccggtctcg atcgcctcga cgaacaggcc gggccggaag atgagcccct
     4261 cgggcgtggg ttggagtccg ccgatcgtct cgaaggtcgt ccactccgag gtcgcggtgg
     4321 tcggctggta gccggtgcac aacatggagt gccggcccag ctcggcggcc agatacgcca
     4381 aggtggtctt gccggtgccc gggggcccgg tcaggatcac gtgcttgccg gcgtcgaggg
     4441 cggcgaccag ctggtcgacg acgtagggag ggaagatcat cccctccgcc tcggccacct
     4501 tggtgagttc ggatgctgag aacgcggtca cacccgttgg ttcggcgcgg cccaccggcc
     4561 ccttgaggaa gctgccgact ctctcagcga cgccgcaccg ccgggtgggg gagccaggtc
     4621 acaccggccg ccggcccgtc gtccacgccg agcgcggcgc gcaacagctc gtatcgctcc
     4681 tcgccgacca gcccgacgat gtcgtcacga caggtggtga cgagcggttc gtgggcggtg
     4741 aacgcctcgg actcctcggt gcaccaccag tggaactcct gtccgccctc accccagtcc
     4801 cggcgacgcc actctcgcag cgtgtgggtg gtgtggccga cttcgtcggt gtggtactcg
     4861 agtcggagcg ggacctgcca gcacacctcg ggcttccagt ccatcggccg ttcgccggcg
     4921 tcgagcgccg ccacgtgcag ggcgcagccc gccccgcggt ggaagtccgg gcggttcaac
     4981 aggatgcagg cgccgtcgtg gacccgggtc acccagtcgc cgtcgtcgtt gcggccgatc
     5041 gccccgccat gcttcctggc ctcgcgccgg aactgccact ggtcgcggtc gagtcggtcg
     5101 atcagcttgc ggacccgcac ccggtcggcc ttgtcggcga agtgcgcacc gaacgagcag
     5161 cagccgtgcc cgaggtgctc ggcgtcctcc tccagcaccc ccttgcagcc atcgttgaag
     5221 atgcagcggt acgggctcat caggaacgtg gcgtcgaaca tccaggtgtc gtcgtcgtgt
     5281 tcgaagctga cccactggcg cggatgggct tcgctgtggg tcttgtcacg gtctcggcgt
     5341 tcggacacgg agggcgacac taccgagcgc ggcgcgccgc gctgtcacca aggggtgcta
     5401 gatcaccccc atggccggtg agttcgacga catccgcacc cgactcgaag cgatctccga
     5461 ggagttggcc gatctggcga tcctccgact gcgagagtcg atcgacgccg gcggcgacga
     5521 gctgccggtc gacgaacggc gactcacccg ggcccggagg gcggtcgaca aggccgccca
     5581 cctgctcgcc gaatccgacg gcgcctgagc cgggaccggc ggtaccgtcc cgcccatggg
     5641 cacggttcac gacgggatcg acgacggact acgggactgg atcgaacgtc aacacgtcgt
     5701 gttcgtcgcg accgcgccca gcgacccgtc cggactggtc aacctgtcac ccaaggggat
     5761 ggtcgacacg ttcaccgtcg tcgaccgcca ccgctgcgcg tatctcgacc tgaccggcag
     5821 cggcgccgag acggtggccc acctgcgcga caacggccgc atcaccgcga tgtggtgcgc
     5881 attcgacggg ccgccgcgca tcgtgcggtg ccacggcacc ggccgggtcg tcacccccga
     5941 cgacgcggag ttcgagtcgt ggcgggagcg gttcggtccg caccgcgggg cccgggccgt
     6001 cgtggtcatc gacgtgatcc gggtcgcgga ctcgtgcggc tacgcggtgc cgctcatgga
     6061 gttcgtcggt gaacgggacc ggctggtccg ctggaccgag agccgcaccg acgaggagct
     6121 ggagcagtac cggcgggatc gcaacgcggt gagcatcgac ggtctccccg ccttcgacaa
     6181 gtggatattg ggcgagttca aggggtgacg gctacaccgt gaggtgaggt ctccaccggt
     6241 cagagcgtga tggcgggcat gatgcccgct gtctacacag caccctgacc agtggagaac
     6301 gccagcatgc cagcccgacc cctgaccgcg cccgaacgcg aagagatccg agcggggatc
     6361 gaacgtcgtg agcccgaccg cgtgatcgcc gggcggctcg gccggcatcg cgccacgatc
     6421 aacgccgaga tcaaccgcaa cggaggtcgg gccggcgtcg ccgcgaccgc cgcgcagacc
     6481 cgtgcggacg cgtgccgggc ctgccccaag gactccaagc tcgaagctga cccggtgctc
     6541 gccgcgcacg tgagcgcacg cttggaggcg aaggactcgc cgatgacgat ctcgatcgag
     6601 ctcgcccgcg gtgtgcacgg cttcgaagcg gagatctccg acgagtgcat ctaccaggcc
     6661 gtctacgccc acggtcgccg tggtctgcgt gccgggctgc acgaggggct gcaccggcgt
     6721 cgccggtgcc gcaaacaccg tgtgatcggg cctccggcac ccaagacgag tccgctcggg
     6781 acgttcaacc tgatcgggct gcgaccggcg gtcgctgctg agcgtgtcga ggtcggtcac
     6841 cgcgaaggcg acctcatcgt cgggtcgttc aaccgctccg cgatcgcgac cgtgttcgac
     6901 cgcgccagcc gccacctctg gcttgcgaac ttccccgaag accacggcgc cgacgcgacc
     6961 ctcgccgcgc tctgcgagat cctcgaccgc attcccgcac cgttgcgcac cctgacctgg
     7021 gaccaaggac gccctacctg tcagtatgag tgtcgctcag tggagtggcc atattcgatg
     7081 agggcgggga ggtgagatca tgaccgtgat gactgatgag acc
//
LOCUS       DAOQOD010000161         7086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4042_length_7086_cov_11.463945, whole genome shotgun sequence.
ACCESSION   DAOQOD010000161 DAOQOD010000000
VERSION     DAOQOD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4042_length_7086_cov_11.463945"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="serC"
                     /locus_tag="PLZ72_13080"
     CDS             <1..1092
                     /gene="serC"
                     /locus_tag="PLZ72_13080"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNA37278.1"
                     /translation="LPGDGRFGCGPSKIRKDSFDMLIGSAGHFLGTSHRQAAVRFKVS
                     ELRNGLAELFSLPDGYEVLLGNGGTTAFWDAATFGLIDSRSQHLEFGEFSSKFAKAAA
                     AAPHLGDPEVISTEPGHHPTAVPMDGIDAYCLTHNETSTGVAMPLERPHGTDGDALVL
                     VDATSAAGGLRFDAAQTDVYYFAPQKCLASDGGLWLAACSPAAIERIERIAASDRWIP
                     AFFDLGIALENSRKDQTYNTPALATLFLATEQVQWILDNGGLEFAAGRCDRSAAAIYD
                     WADGHSHASPFVTDPAMRSHVVATIDFDDSVDAATVAAVLRDNGIVDTEPYRKLGRNQ
                     LRVALFPAIDPEDVVQLTRCIDFVLEQLA"
     gene            1204..2946
                     /locus_tag="PLZ72_13085"
     CDS             1204..2946
                     /locus_tag="PLZ72_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA37279.1"
                     /translation="MPTAQEIRAQLTGPGGPFEIVEETVEGTPMLVYKERFNDLRVVA
                     AFGQSHGEKTFIVHGDERITFTDFMRNANSVSRRLGEFGVGHGDRVAVLSANNPEWCL
                     SFWGTVDIGAILVGLNGWWITDEIVYGLTDSGARVLVADRRRFERVAEEVDRIDTLEH
                     VFLIDADPSEFAAADGSVSPKLHRFDELLTDPTDELPTVPIDEGDAAVIFYTSGTTGR
                     PKGAISTHRSMIANLQNTVFLLTAGAMGQPADDREADAPPTDGSGQPVSLLTSPLFHV
                     SGCHSGLVVGMMAGIRLVILPGRFTPEAAFDAIERERVMIWATVPTMIWRACEHPDRH
                     DRDLRSIIAVSFGGSPSADELQRRIRETFPNVKATTNAYGLTESSSVATALAGDDALR
                     KPDSVGLPVPNTQIAIAGPDGTHLGPGETGEVLIRGPLIMAGYWNKPEATASTVIDGW
                     LHTGDVGHLDDEGYLYITDRAKDMLIRGGENVYCVEIENRLVEHPAIADAAVVGVPHD
                     GLGEEVKAVIEVADGADISDDEVRAWVADTLAAFKVPTYVQRWDGKLPRNASGKLLKN
                     VIRGEGAVSFAETM"
     gene            3068..4768
                     /locus_tag="PLZ72_13090"
     CDS             3068..4768
                     /locus_tag="PLZ72_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNA37280.1"
                     /translation="MFRTLPIPTLWRSSAPALTLPPPGRLDRSRPTLRQRFRDPGTRR
                     VFAVVLAGKLLGVLSLLAIIKALSSIFGAEAGASGLSPAAVDAGEIINPINTLWVMIA
                     AFLVFFMQAGFMALEAGVARSRETVNVLMECVFDTCLCGLLFWAVGFAFMFGEGNGLI
                     GHQYFFLNGATGGYGTTGVAFLAFFVFQLAFADAASTITSGAMVGRTSFKGDILYSIA
                     VSGLIYPIFGHWVWGPGGWLGNTMGWFSGLVPDGIVFRDFAGSTVVHSVGGIVALAGA
                     VCLGPRLGRRFARDGGGLFPPHDLVMASIGAVILWFGWYGFNPGSTLSAMDWEGIGRV
                     AANTTLAACAGGMVAVFFVYPRSGKWDLGMSLNGFLGGLVAITAPCYWVSPLGAVLIG
                     TVAGVIVPLGVDLLERLRIDDPIGAVPVHLVCGVFGTLAVGLFATGEYGLPTPEGADN
                     SSPITGLLYGGGVGQLVAQLIGSLTCLVVVGAVAFGLMKALRMLPGSWNLRLSRDEEL
                     EGIDVVEHGLPPYHLESGAGVVYVTPTSPNFDAPPPARRDRGAEEGSDDEGSKVSESV
                     "
     gene            5000..6907
                     /locus_tag="PLZ72_13095"
     CDS             5000..6907
                     /locus_tag="PLZ72_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNA37281.1"
                     /translation="MDRRRFLTALGAGAGIAAVGLPMSGCAAPPAPDPLAGGPFGAPT
                     PTPGRDRWARVPSAFNRRVTAEQATTPLTLLDGELPSDMAGHVFFQSLSLRDTDAGFS
                     GDSLVWRVDLDGEVPTITSRLLRTTDLLLHDAVADTDLRFESRGVSRLGPLGMQDQVN
                     TALVPLNGNRLVATVDGGRSWELDPATLAPVGPVGRLDDHRPLVDLPVNRFLCPLNIT
                     SAHPPYDAETGEYYGVSLSIVPIPGLVYCEILCWDGVGAIKRVPLTTPDGAPVLLSQS
                     AHQMCISRDHLLIADTSTVIEFGKLMYPPHSAEAAEAQTPRPDTVLFIVSRDELRRTA
                     GGATAVRAVIPRESGHLMVDHDAAPGRIVVHSAHTSAMDFAEWVMPTDRHPVTGEPVR
                     SDLVDAITPVSYDIGVVGRYEIDTRTGAVLDQHAFFDDHTWGSGGLTARNPLTPIDTV
                     GDVFHANSGMPTDIAVQRMHAVFTSYPHRIVPDAELPWSGVPTSLLRIDHDAGRVVDA
                     YWHAGDRFGWTPTFVPRRGTATGSTDGYVVSVVYSDHADARSSGTELWVFDAADLARG
                     PIARLGRADLAMPLTLHSTWLDSLRASRPDTRVDVAAELTERADTWLADPRVASIVRN
                     DVLPAYDAVRV"
BASE COUNT         1003 a         2635 c         2393 g         1055 t
ORIGIN      
        1 ctccccggcg acggccgctt cggctgcggc ccgtcgaaga tccgcaagga ctccttcgac
       61 atgctgatcg gatccgccgg ccacttcctc ggcacctcgc accgccaggc cgccgtgcgg
      121 ttcaaggtgt cggagctccg aaacgggctg gccgagctgt tctcgctccc cgacggctac
      181 gaggtgctcc tcggcaacgg cgggaccacc gcgttctggg atgccgccac cttcggtctc
      241 atcgactcgc gcagccagca cctggagttc ggcgagttca gctccaagtt cgccaaggcc
      301 gccgccgcgg caccgcacct gggcgatccc gaggtgatca gcaccgaacc cggacaccac
      361 cccaccgcgg tgcccatgga cggcatcgac gcctactgcc tgacccacaa cgagacctcc
      421 accggcgtcg ccatgcccct ggaacggccg cacggcaccg acggcgacgc cctggtgctg
      481 gtcgacgcca cctccgccgc cggtggactg cgcttcgatg ccgcccagac cgacgtctac
      541 tacttcgcac cccagaagtg cctggcctcc gacggcggac tgtggctggc ggcgtgctcg
      601 ccggccgcga tcgagcgcat cgagcgcatc gcggcgtcgg accggtggat ccccgcgttc
      661 ttcgacctgg gcatcgcgct cgagaactcg cgcaaggacc agacctacaa caccccggcg
      721 ctggcgacgc tgttcctggc caccgagcag gtccagtgga tcctcgacaa cggcgggctc
      781 gagttcgcgg ccggccgctg cgaccgctcc gcggctgcca tctacgactg ggccgacggc
      841 cacagccacg cgtcgccgtt cgtgacggac ccggccatgc gctcccacgt cgtggccacg
      901 atcgacttcg acgacagcgt cgacgcggcc actgtcgcgg cggtgctgcg cgacaacggc
      961 atcgtcgaca ccgagccgta ccgcaagctg ggccgcaacc agctccgcgt cgcgctgttc
     1021 ccggcgatcg accccgagga cgtggtccag ctcacacgct gcatcgactt cgtgctcgaa
     1081 cagctcgcct gatcgtccgg tcgggtggat ccccgaccga cgattcggcg cgagactccc
     1141 ccacacggtc cgccgagggg cggcgggcag ggccgtcacc cgacggccgg accaggggga
     1201 cacatgccga ccgcacagga gatccgcgcg cagctcaccg ggccgggcgg tccgttcgag
     1261 atcgtcgagg agaccgtcga gggcaccccg atgctcgtct acaaggagcg cttcaacgac
     1321 ctgcgggtgg tcgcggcgtt cggccagtcc cacggtgaga agaccttcat cgtccacggc
     1381 gacgagcgca tcacgttcac cgacttcatg cggaacgcca actcggtcag ccgtcggctc
     1441 ggcgagttcg gcgtcggcca cggcgaccgg gtggcggtgc tgagcgcgaa caaccctgag
     1501 tggtgcctga gcttctgggg caccgtcgac atcggggcga tcctcgtcgg actcaacggc
     1561 tggtggatca ccgacgagat cgtctacggc ctcaccgact ccggagcgcg ggtgctggtc
     1621 gccgaccgtc gccgcttcga gcgtgtcgcc gaggaggtcg accgcatcga caccctcgaa
     1681 cacgtcttcc tcatcgacgc ggacccgtcg gagttcgccg ccgccgacgg gtcggtcagc
     1741 cccaagctgc accgattcga cgagctgctc accgacccga ccgacgagct gccgacggtg
     1801 ccgatcgacg agggcgacgc cgcggtcatc ttctacacct ccggcacgac cggtcgaccc
     1861 aagggcgcca tctcgacgca ccgctcgatg atcgccaacc tccagaacac ggtgttcctg
     1921 ctgaccgccg gagcgatggg ccagccggcc gacgaccgtg aagccgacgc cccgcccacc
     1981 gacggctccg gtcaaccggt gtcgctgctc acctctcccc tgttccacgt gtcgggctgc
     2041 cactcgggcc tggtcgtcgg gatgatggcc gggatccggt tggtgatcct gccgggacgg
     2101 ttcacgcccg aggccgcgtt cgacgccatc gagcgggaac gggtgatgat ctgggcgacg
     2161 gtgcccacca tgatctggcg ggcgtgcgag caccccgacc gccacgaccg cgacctgcgc
     2221 tcgatcatcg cggtcagctt cggcggctcc ccctcggccg acgagctgca gcggcgcatc
     2281 cgcgagacgt tccccaacgt gaaggcgacc accaacgcct acggcctgac cgagtcgtcg
     2341 tcggtggcca ccgccctggc cggcgacgac gccctccgca agcccgactc ggtcggcctg
     2401 cccgtgccca acacccagat cgccatcgcc gggccggacg ggacccacct gggcccgggc
     2461 gagaccggcg aggtgctgat ccggggaccg ctgatcatgg ccggctactg gaacaagccc
     2521 gaggcgacgg catcgacggt gatcgacggg tggctgcaca ccggcgacgt cggccacctc
     2581 gacgacgagg gctacctcta catcaccgac cgggccaagg acatgctgat ccggggcggc
     2641 gagaacgtct actgcgtcga gatcgagaac cgcctcgtcg agcaccccgc catcgcggac
     2701 gccgcggtgg tcggcgttcc ccacgacggg ttgggcgagg aggtcaaggc ggtcatcgag
     2761 gtcgccgacg gcgccgacat ctccgacgac gaggtgcggg cctgggtggc cgacacgctc
     2821 gccgcgttca aggtcccgac ctacgtgcaa cgctgggacg gcaagctgcc ccgcaacgcg
     2881 tcgggcaagc tgctcaagaa cgtgatccgc ggcgaaggcg ctgtcagctt cgccgagacg
     2941 atgtagcgat cgagccaaca ccgcgttcgg cgtgagatgc cgtcgaaacg gcggcgaaac
     3001 acgaccgacg ttcgggcttc accggcgaca cacggggccg aaacacgggc tccgtaccgt
     3061 gccccgcatg ttccgaaccc tgccgatccc caccctgtgg cgctcctcgg cgcccgccct
     3121 cacactgccc ccaccgggcc gactcgaccg gtcccgcccg acgttgcgtc agcggttcag
     3181 ggaccccggg acccgcaggg tgttcgcagt ggtgctcgcc ggcaagctgc tcggcgtcct
     3241 gtcgctgctc gccatcatca aggcgttgag ctcgatcttc ggcgccgagg cgggcgcatc
     3301 gggactgagc cccgccgcgg tcgacgccgg ggagatcatc aacccgatca acacgctgtg
     3361 ggtcatgatc gcggcgttcc tggtgttctt catgcaggcg gggttcatgg ccctcgaggc
     3421 cggcgtcgcc cgctcccgcg agaccgtcaa cgtcctgatg gagtgcgtgt tcgacacgtg
     3481 cctgtgcggg ttgctgttct gggccgtcgg cttcgccttc atgttcggcg agggcaacgg
     3541 gctgatcggc caccagtact tcttcctcaa cggcgccacc ggcggctacg gcaccaccgg
     3601 ggtggccttc ctggcgttct tcgtcttcca gctcgcgttc gccgacgcgg cgtcgaccat
     3661 cacatcgggg gccatggtcg gccgcaccag cttcaagggc gacatcctct actcgatcgc
     3721 ggtgtcgggg ttgatctacc ccatcttcgg ccactgggtg tggggtccgg gcgggtggct
     3781 cggcaacacc atgggctggt tctccggcct ggtccccgac gggatcgtct tccgtgactt
     3841 cgccggatcc accgtggtcc acagcgtggg cggcatcgtc gcccttgccg gagcggtgtg
     3901 cctgggccca cgactcgggc ggcgcttcgc ccgcgacggc ggcggtctgt tcccgcccca
     3961 cgacctggtg atggcctcga tcggcgccgt gatcctctgg ttcggctggt acggcttcaa
     4021 ccccggctcg actctctcgg cgatggactg ggagggcatc ggccgggtgg ccgccaacac
     4081 cacgctggcg gcgtgcgccg gcgggatggt cgccgtgttc ttcgtctacc cccggtccgg
     4141 caagtgggac ctgggaatgt cgctcaacgg cttcctcggc ggcctggtcg ccatcaccgc
     4201 cccgtgctac tgggtcagcc cgctgggggc cgtgctcatc gggaccgtcg ccggcgtcat
     4261 cgtgccgctc ggcgtcgacc tgttggagcg gctgcgcatc gacgacccga tcggagcggt
     4321 gccggtccac ctcgtctgcg gcgtgttcgg caccctcgcg gtcggcctgt tcgccaccgg
     4381 cgagtacggc ctgcccaccc cggagggcgc cgacaacagc tcaccgatca ccgggctgct
     4441 ctacggcggc ggggtcggac agctcgtcgc ccagctcatc gggagcctca cctgcctggt
     4501 cgtcgtcgga gcggtggcgt tcgggctgat gaaggccctg cggatgctgc cgggatcctg
     4561 gaacctgcgt ctcagccggg acgaggagct cgaggggatc gacgtcgtcg agcacggcct
     4621 gccgccgtac cacctcgagt cgggtgcggg tgtcgtgtac gtgacgccga ccagccccaa
     4681 cttcgacgcc ccaccgccgg cccgccggga ccggggcgcc gaggaaggct ccgacgacga
     4741 gggctcgaag gtgtccgagt cggtctgacc cgcttcggcg cctcgggttc tcatgacagt
     4801 tgagtaccgc cactgtcgtg acttgtcgtc agaacgcggg gtgggggtgg gcgggggcgt
     4861 ggtggccggt ccggaacacc ggtcggggag acgtccgagc ccctaccctg ggaccactgg
     4921 tcggcggact ggaccccgga cccgccgtga cggtgaagga ccgggtctcc ggaggggaat
     4981 ccaggagggg gacacagcca tggaccgcag acgcttcctg actgcgctcg gcgcgggtgc
     5041 cgggatcgca gcggtgggat tgccgatgtc ggggtgcgcc gcgccgccgg cgccggaccc
     5101 gctcgccggc ggaccgttcg gcgcccccac ccccacaccc ggccgtgacc gctgggcccg
     5161 ggtgccgagc gcgttcaacc ggcgggtcac cgccgagcag gcgacgactc cgctgacgct
     5221 gctcgacggg gagctcccgt cggacatggc cggccacgtg ttcttccagt cgctgtcgct
     5281 gcgcgacacc gacgccggct tcagcggtga ctcgctggtg tggcgcgtcg acctcgacgg
     5341 cgaggtgccc acgatcacgt cgcgtctgct gcgcaccacc gacctgctgc tgcacgacgc
     5401 cgtggccgac accgacctgc gcttcgagtc ccgcggcgtg tcccgactcg gcccgctcgg
     5461 catgcaggac caggtcaaca ccgcgctggt gccgctgaac gggaaccgcc tggtcgccac
     5521 cgtcgacggc ggccgctcct gggagctcga ccccgccacc ctcgctccgg tcggaccggt
     5581 cgggcgcctc gacgaccacc gaccgctcgt cgacctgccg gtcaaccgct tcctgtgccc
     5641 gctcaacatc acgagcgccc atccgccgta tgacgccgag accggcgagt actacggggt
     5701 gtcgctgtcg atcgtcccga tcccgggcct ggtgtactgc gagatcctct gctgggacgg
     5761 cgtcggcgcg atcaagcggg tgccgctgac cacccccgac ggcgcaccgg tgctgctgtc
     5821 gcagtccgcg caccagatgt gcatcagccg cgaccacctg ctgatcgccg acacgtcgac
     5881 ggtgatcgag ttcggcaagc tcatgtaccc gccgcactcc gccgaggccg ccgaggccca
     5941 gaccccccgt cccgacaccg tcctgttcat cgtgagtcgc gacgagctgc gccgcaccgc
     6001 cggcggcgcc accgcggtgc gggcggtcat cccccgcgag tcgggccacc tgatggtcga
     6061 ccacgacgcc gcgccgggtc gcatcgtggt gcacagcgcg cacacctcgg cgatggactt
     6121 cgccgagtgg gtgatgccga ccgaccggca cccggtcacc ggcgagccgg tgcggtccga
     6181 cctcgtcgac gccatcaccc cggtcagcta cgacatcggc gtcgtcggcc gctacgagat
     6241 cgacacccgc accggggcgg tgctcgacca gcacgccttc ttcgacgacc acacctgggg
     6301 cagcggcggg ctgaccgccc gcaacccgct gacgccgatc gacaccgtcg gcgacgtgtt
     6361 ccacgccaac tccgggatgc cgaccgacat cgcggtgcag cgcatgcacg cggtgttcac
     6421 ctcgtacccg caccggatcg tccccgacgc ggagctgccc tggtcggggg tgccgacgtc
     6481 gctgctgcgc atcgaccacg acgccggcag ggtcgtcgac gcctactggc acgccggtga
     6541 ccgcttcggc tggaccccca cgttcgtgcc ccggcggggc acggcgaccg gctcaaccga
     6601 cggttacgtc gtctcggtcg tgtactccga ccacgccgat gcgcgcagct cgggcaccga
     6661 gctgtgggtg ttcgacgccg cggacctggc ccggggtccg atcgcccggc tcggtcgggc
     6721 cgatctggcg atgccgctca cgctgcactc gacgtggctc gactcgctgc gtgcgtcacg
     6781 acccgacaca cgggtggacg tcgccgcaga gttgaccgag cgggccgaca cctggctggc
     6841 cgacccgcgg gtcgcctcga tcgtgcgcaa cgacgtgctg cccgcctacg acgccgtccg
     6901 ggtctgaggt ccggccccgg accgggcacc cggctcagat cgggacgccg tcgtcgatct
     6961 cggcggcgac ctcgaggacg cacgcggcca ggtcgtcgtc gagctcgccg acgcggtcga
     7021 gctcgtcggc gaagcgcagc cggggccgct cctgcgccgg gaccgcccgc ggcatccacg
     7081 ccgagt
//
LOCUS       DAOQOD010000162         7083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4044_length_7083_cov_13.636312, whole genome shotgun sequence.
ACCESSION   DAOQOD010000162 DAOQOD010000000
VERSION     DAOQOD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4044_length_7083_cov_13.636312"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="cofH"
                     /locus_tag="PLZ72_13100"
     CDS             <1..947
                     /gene="cofH"
                     /locus_tag="PLZ72_13100"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNA37282.1"
                     /translation="NINYTNVCTFKCRFCGFSKGPLSLNLRGNPYLLTLDDIAARAAE
                     AAELGATEVCLQGGIHPDFDGDYYIEVTRAVKDAAPDLHVHGFTALEVTEGARRLGEN
                     LEEYLGRLRDAGLRTLPGTAAEILDDDVRAIICPDKIDTQQWLDAHRSAHRVGLNSNV
                     TMMFGSVEHPRSWARHLLITRDLQRETGGFTEFVGLPFVHMAAPLYLQHRGRRGPTFR
                     EVVLVHAVARITYRELIPHVQASWVKLGLAGVSQLLQAGADDLGGTLMDENISRAAGA
                     EHGTMATPEDFRALVEPLGRTLVQRSTLYGRVPAPAPA"
     gene            complement(914..2017)
                     /locus_tag="PLZ72_13105"
     CDS             complement(914..2017)
                     /locus_tag="PLZ72_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37283.1"
                     /translation="MSSSVAETPATDSPGAQVLAGLAVPGLDLDKARHMVDVRAARRL
                     RRRRTVWGAAAAVVLVGIAVWAWPRPDPQELVADGDRDPTTTTTTPVTASSTVPVLTT
                     VVPTTVPSSTTAAPTVTTVPPTTSTTTAPNQPMAVTAQIVPTPGGAPPRVGETVTVRV
                     NWSDPDLADPATVDVRADFGDPLVTRALSTAARPPCTARGGGAAALVDLPFRYASSGE
                     HWVQVVVTACDGVGPYGERKVLEIPVQVQAPETGRRAVVIGGGDGRSPDSAAVLAGVE
                     TVARRDPELTQVLPDGTTRATVAVIDAAYAGPLHLRWGEPPGASCQVTASDQSVTAVE
                     PPDSRRDHAVGVRLAPGPAACPTQAGAGAGTRP"
     gene            complement(2014..2622)
                     /locus_tag="PLZ72_13110"
     CDS             complement(2014..2622)
                     /locus_tag="PLZ72_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37284.1"
                     /translation="MSTQTVEVPGAGRAPSEFDRLFARTYPAVVGLARRVLDRDGVAS
                     SASEPIADEVAVEALSRARVHHLGDTDRAAERTVGWAAELCLDRIIGHPGRVALPPEV
                     GADRLLSADLLEDGVGAEWDDHGLALWELQEALAGARRVDRRIGVLCLGCGLPPRQVG
                     ALLGLDDRAVTAGLDRIGVRLSDRRRVSAEVESPLDARLGDQ"
     gene            2702..3739
                     /locus_tag="PLZ72_13115"
     CDS             2702..3739
                     /locus_tag="PLZ72_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNA37285.1"
                     /translation="MTERRPRYRTGDDDLDRRIDELIADAGIQADQDLAFELVTSVLR
                     MGREGLSRGDLKIANSTLKEMRYAFHVFDPYRSVRKLAIFGSARTGIDEPAYAAARDV
                     GREIADEGWMVITGGGPGIMTAGIEGAGAENSFAVNIVLPFEPAGGGVMVNDGKVINF
                     KYFFNRKLTFMKEACAYVMFPGGYGTMDETFELLTLLQTGREVPAPIVLFEPPGDAYW
                     RSFRHFLEVELLDSGLIAADDLDLFHITSDVGDAVEHLTAFYRVFDSIRYVGGRLVLR
                     LSKELTDAQLEKLNDEFADIVASGRIERAAASPAEIADGDAVDRPRIRFRFIDNSYAR
                     LHAMIRLINTF"
     gene            complement(3939..4307)
                     /locus_tag="PLZ72_13120"
     CDS             complement(3939..4307)
                     /locus_tag="PLZ72_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37286.1"
                     /translation="MTAPRKPKRLLSGEQKYDLWVRMLTEQITPAQAAAEAGVDRSTI
                     SNLRKTAKDGAIAALTAAKPGRRKSTAEVSEAARLQAEIDRLGMVVIPFDRDLNTAVN
                     LARWPDRPASTGSPPLPTAA"
     gene            complement(6738..7004)
                     /locus_tag="PLZ72_13125"
                     /pseudo
     CDS             complement(6738..7004)
                     /locus_tag="PLZ72_13125"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1169 a         2369 c         2352 g         1193 t
ORIGIN      
        1 gcaacatcaa ctacacgaac gtctgcacgt tcaagtgccg gttctgcggc ttctccaagg
       61 gtccgctgag cctgaacctg cggggcaacc cgtacctgct cacgctcgac gacatcgctg
      121 cccgagccgc ggaggccgcg gagctgggtg cgaccgaggt ctgcctccag ggcgggatcc
      181 accccgactt cgacggcgac tactacatcg aggtgacccg ggcggtgaag gacgcggcgc
      241 ccgacctcca cgtccacggc ttcaccgcgc tcgaggtgac cgagggcgcc cgccggctcg
      301 gcgaaaacct cgaggagtac ctgggtcgtc tgcgcgacgc cgggctgcgg accctgccgg
      361 gcaccgcggc cgagatcctc gacgacgacg tccgggcgat catctgcccc gacaagatcg
      421 acacccagca gtggctcgac gcccaccgca gcgcccaccg ggtcgggttg aactcgaacg
      481 tgaccatgat gttcggctcc gtcgagcacc ctcgcagctg ggcccggcac ctgttgatca
      541 cccgtgacct gcagcgcgag accggcgggt tcaccgagtt cgtcggcctg ccgttcgtgc
      601 acatggccgc cccgctctac ctccaacacc gggggcggag aggcccgacg ttccgcgagg
      661 tggtcctggt ccacgccgtg gcccgcatca cctaccggga gctgatcccg cacgtgcagg
      721 cgtcgtgggt caagctcggt ctggccgggg tctcccagct gctgcaggcc ggcgcggatg
      781 acctgggcgg caccctcatg gacgagaaca tcagccgtgc ggcgggggcc gagcacggca
      841 cgatggccac ccccgaggac ttccgtgccc tggtcgagcc gctcgggcgg accctggtgc
      901 agcgcagcac cctctacggc cgggtccccg ctccggcccc cgcctgagtc ggacacgcgg
      961 ccggtccggg cgcgaggcgt acgccgaccg cgtggtctcg ccgggagtcg ggcggttcga
     1021 cagcggtgac cgactggtcc gacgctgtca cctggcacga cgcgccgggg ggctcgcccc
     1081 agcgcaggtg cagcggaccg gcgtaggcgg cgtcgatgac cgcgacggtg gcccgggtgg
     1141 tgccgtcggg cagcacctgg gtgagctcgg gatcgcgacg ggcgacggtc tcgacgccgg
     1201 cgagcacggc tgcggagtcc ggtgagcggc cgtcgccccc tccgatcacc accgccctcc
     1261 gcccggtctc gggggcctgg acctggaccg ggatctccaa caccttgcgc tcgccgtagg
     1321 ggccgacgcc gtcgcacgcc gtcaccacga cctgcaccca gtgctccccg gacgacgcgt
     1381 agcggaacgg caggtcgacc aacgccgcag cgccgccgcc gcgggcggtg cacggaggcc
     1441 gggcggcggt gctgagcgcc cgggtgacga gcgggtcgcc gaagtcggcg cggacgtcga
     1501 cggtggccgg gtcggccagg tcggggtcgc tccagttcac ccggaccgtg accgtctcgc
     1561 cgacccgggg cggggcgccg ccgggggtcg ggacgatctg ggcggtgacc gccatcggct
     1621 ggttgggtgc ggtcgtggtg ctggtcgtcg gggggaccgt ggtcaccgtc ggggccgcgg
     1681 tcgtgctcga cgggaccgtc gtcgggacga cggtggtgag gaccggaacg gtgctcgacg
     1741 cggtgaccgg ggttgtggtc gtcgtcgtgg ggtcccgatc gccgtcggcg acgagctcct
     1801 gggggtccgg tcgcggccac gcccacaccg cgatcccgac caggaccacc gcagccgccg
     1861 cgccccagac ggtgcgacgg cgacggagcc gccgggcggc ccgcacgtcg accatgtggc
     1921 gtgccttgtc caggtcgaga cccggaacgg ccagtccggc caggacctgg gcccccggcg
     1981 agtcggtcgc gggtgtctcg gcgaccgaac tgctcactgg tcccccaggc gggcgtcgag
     2041 agggctctcg acttcggcgg agacccgacg tcggtccgac aggcgtacgc cgatccggtc
     2101 gaggccggcg gtcacggccc ggtcgtcgag tccgagcagc gctcccacct ggcggggcgg
     2161 cagaccacag cccaggcaca gcaccccgat gcgccggtct acgcgccgcg cgccggccag
     2221 ggcctcctgg agctcccaca gggcaagccc gtggtcatcc cactcggcac cgacgccgtc
     2281 ctcgaggaga tcggcggaca gcagccggtc cgcacccacc tcgggcggca acgccacccg
     2341 gcccggatgg ccgatgatgc ggtcgaggca cagctcggcg gcccacccga cggttcgctc
     2401 cgccgcccgg tcggtgtcgc cgaggtgatg gacccgggcc cggctcagcg cttcgaccgc
     2461 gacctcgtcc gcgatcggtt ccgacgcgga tgaggcgaca ccatcacgat cgagcacccg
     2521 gcgggcgagt ccgaccaccg cggggtaggt ccgggcgaag aggcggtcga actcgctcgg
     2581 ggcgcgtccg gcccccggga cctcgaccgt ctgcgtgctc acggtcgcgg attgttccac
     2641 ggcggcgttg ccgagggtgt gatgggccag gtggcgtcgg gtcggtacgg ttggccggac
     2701 catgaccgaa cgacgaccgc gctaccgcac cggcgacgac gacctggacc gtcggatcga
     2761 cgagctgatc gcggatgcag ggatccaggc ggaccaggac ctggccttcg agctggtgac
     2821 ctcggtgctg cgcatgggtc gtgagggcct gtcgcggggt gacctcaaga tcgccaactc
     2881 gacgctgaag gagatgcgct acgcgttcca cgtgttcgac ccctaccggt cggtgcggaa
     2941 gctggcgatc ttcggctcgg cgcgcaccgg catcgacgag cccgcctacg ccgcggcgcg
     3001 cgatgtgggt cgtgagatcg cggacgaggg ctggatggtg atcaccggcg gcggtccggg
     3061 gatcatgacc gccggcatcg agggggccgg ggccgagaac agcttcgcgg tcaacatcgt
     3121 gctgcccttc gaaccggccg gcggcggtgt gatggtcaac gacggcaagg tcatcaactt
     3181 caagtacttc ttcaaccgca agctcacgtt catgaaggag gcgtgcgcgt acgtcatgtt
     3241 ccccggcggg tacgggacca tggacgagac cttcgagctg ctgacgctgc tgcagaccgg
     3301 tcgcgaggtg ccggccccga tcgtcctgtt cgagcccccg ggcgacgcct actggcgcag
     3361 cttccgacac ttcctcgagg tcgagctgct cgactcggga ctgatcgcag cggacgacct
     3421 cgacctgttc cacatcacct ccgacgtggg cgacgcggtc gagcacctca ccgcgttcta
     3481 ccgggtcttc gactcgatcc gctacgtcgg cgggcgcctg gtgctgcgtc tgagcaagga
     3541 gctgaccgac gcacagctcg agaagctcaa cgacgagttc gccgacatcg tggcgtcggg
     3601 tcgcatcgaa cgggccgcgg cctccccggc cgagatcgcg gacggggacg ccgtcgaccg
     3661 cccccgcatc cggttccggt tcatcgacaa ctcctacgcc cgcctgcacg cgatgatccg
     3721 gctgatcaac acgttctgac agcgtgtgtc ctgttcggac gcaccggctc cccttaacca
     3781 ccgaccgtgt gtcggtggtg tttggttgcc atccggacgc cccacctgag caaggacttc
     3841 ttccggttcc gatccagtgc ccgggtccca ccgcagtggg ggtgtgggtg acgatcggcg
     3901 tttcccgagt gcttcccggt gcagaccctt gaagggggtc aggctgccgt tggtagcggc
     3961 ggagatcctg tggatgctgg ccggtcgggc cagcgtgcga ggttgaccgc ggtgttcaag
     4021 tcgcggtcga aagggatcac gaccatcccc aaccggtcga tctcggcctg caacctcgca
     4081 gcctcggaca cctccgcggt gctcttgcga cgaccaggct tcgccgcggt caacgccgcg
     4141 atcgcaccgt ccttcgccgt cttgcgcaga ttcgaaatcg tcgagcgatc cacccccgct
     4201 tccgcggcgg cctgagccgg agtgatctgc tcggtcagca tccgcaccca caagtcgtac
     4261 ttctgctccc ccgacaacaa cctcttcggt ttgcgcggcg cggtcatggc gctgctcctc
     4321 tttcaaggcc agcaaccctg ccaactgcgg tctgaaagaa cacggaccct gtcgcgcgaa
     4381 gtcagatgca acacaagccc caacccgacg ctgacgaccc ggctccgcca cgtcatcgac
     4441 cagacccctc gactcctgca ccgccttgta caccgacgcc tccaccaccc gcgcctggcc
     4501 accccgaccc aacgccaccg ccaaaccccc caccacctga cgacgatccc gcttacccag
     4561 actcggcaac accgacccaa agaacccctt caacgcctcc agcgtctctc catcaccacc
     4621 caccctacac cccgcgacta actcccgggt ggaccctaaa gtttggctgt ccattgtgcg
     4681 aaagcatagc aatgatcact tgggggaggg ctcccgccac agtaaggcga gattacactc
     4741 gccccattag cggccggcaa tgacaaatac aagtagtcga actgcgttgt ggggcctgag
     4801 gatgggaacg ttgcgttgta tacgccagcc aagtagttgg acgagtagac acttggcacg
     4861 gtccctggag ttaagttgaa gtcgccacct agcacacgag gctcggcctt ccatgcggtg
     4921 gcaacgaacg tgtaagcatt ggcctgttgc tgcgcgatag aagtatttga gtttagaaga
     4981 tgcgtcgagc aagctgtgta gagaagcccg aacacgttgg cctgaatgca gatcattcct
     5041 cggcactcgt aggatgacga tggcaggcag ctgctgggtg caccacctcc ttgcggagtg
     5101 taatagtacg cataggtagt gacagggttc ggagccgaga ctacgactcc gtaactcgcc
     5161 ccgccacacc ctgcaacagc tagtcttgtt ggtaaccagt aggcggcata cttgaagctg
     5221 attggatccc cgctgcagta ttcgttcacc gacgttgccc agggtgatgt gctattgaac
     5281 caggagatga ggttcgatgc tgtcgcatga ttcccacagc tagctgaaca catgttgtat
     5341 tgctgggcag tgagcgtcac tgctgtcgca ccaacatccg gagtggcgcc aacaagcacg
     5401 gaccctgtag ccagggcggc cacgagagcc atacggttaa tcaggtttga tatgccaatc
     5461 caattcgaca tggttcggcg ggtcctggcg ctttggcact gatggcttct catgcgcttg
     5521 tccgttggca ctgtgtgcgg gcagggtctt gactcttcat tgggtttgca taggagatgc
     5581 aggcgaatat ggggccgccc gaatcatcta caagaagctg tgctggggca gcgtcggggc
     5641 tcggagcgga gaagccgagc ccagtggact tgccgttcgg acctcgatta atgagttctc
     5701 cgctgggtgt aagtgcgtaa gtctgttgag tacttgaggt attgaacgaa gcgtcaggat
     5761 taggtgcgga cggctcaaga gttgtaaatc cggtggtcgg ggtccagctc ccatgagcga
     5821 tgtcgccaga ttgcgtgaga attgcttggc tcccgcaagt aatcgccgct ggcgcaggta
     5881 tgtcctgttc tatggcgccg cccggctgaa cctgccatcc gccgccttcg tatttcgcga
     5941 tcgagccaac gacgcctgta gtcgttccgg ctgcagggag tagttgcggc gtagatcccg
     6001 tttgctcttg aaccgcaatc gcatagagtg cgtcaccaat cgggcacact cggccgggcg
     6061 gtggccacgg tgcgagtggg gtcaactcgc cattttgacc gagggcctgt attccagaat
     6121 cggccaagag caccgcttgt ccattcatca aacccgcaag gccaagccct gtcgtttcct
     6181 cgggcgccga actgccgcgg gccacttctg tagttccgag gttctttcct gacggtaaga
     6241 ggaccctagt cacttcggcc acccaaccgg cgcagggatc aggtgtcgta cacctgatcc
     6301 cacccacaag cgcaacgtcc cctatagagg tagcgatcgg cgagcggtag gccgacaaag
     6361 tcgctacctg ttcgagccct gaatccttga acgcgtacaa cccggcggac gtatccccga
     6421 ccgcgtagac gaagccaccc ccgggcgctg agatgaccct ggccaccgta ccgatggtgg
     6481 ttgtgttcgg aacggcagcg ttggcgcgta cttccgcttg gtctccgcag cctgatatcc
     6541 agccgagaag cgcgaatacg cacgcgatcg accatcccgt gcgcatcgcc caccgcccag
     6601 ctggggcggc gcccagcggc acccagactg tgggtcgact tcgccagact cggtgaacca
     6661 cccggccagg tttcatttct gcacctccta agggctcacc tcaggactac ttgcacgctc
     6721 ggtgagtctc ggcgagttca accggttatc gcgacagctg catggtagcg gtcagcggcg
     6781 gtgtcccagt tgaggctgtg gcgggggatt gtgttgatgc ggtgctcgat gcggcgtagg
     6841 tcgtgggcgc tgtgagttcg caggtcggtg ctcttgccga cgtagcggcg gatgaggccg
     6901 ttgccgttct cgttggtcgg gcgttgccac ggcgagtgcg gctcggcgaa gtacacgtcg
     6961 atcccgcaca gctcggcgag gtctgaggtt gacccggttt gacggacacc taacttggcc
     7021 tgatgagttc agaacggagt gttcatgtca gcgaagtcaa gcggtagcag acggaagttc
     7081 acc
//
LOCUS       DAOQOD010000163         7067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4062_length_7067_cov_12.139047, whole genome shotgun sequence.
ACCESSION   DAOQOD010000163 DAOQOD010000000
VERSION     DAOQOD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4062_length_7067_cov_12.139047"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..688)
                     /locus_tag="PLZ72_13130"
     CDS             complement(224..688)
                     /locus_tag="PLZ72_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37287.1"
                     /translation="MREIYTALTGRDLPEAMPPRERRTIDAVLTHPDGTRRLVEIDEK
                     QHFMPPRAVVLDHFPDDLPTGFDAPEWAARARAAKRLPGGGFARPCPPLFPDPGGRHL
                     QRAFRDGLADLLPSVHGWRPTLRIADFEVVDWIHAADVADRMAALVGRRLAT"
     gene            complement(772..1470)
                     /locus_tag="PLZ72_13135"
     CDS             complement(772..1470)
                     /locus_tag="PLZ72_13135"
                     /inference="COORDINATES: protein motif:HMM:NF039405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6308 family protein"
                     /protein_id="HNA37288.1"
                     /translation="MQDDYGEFVDAVMAVVTADGGSRARSDVATYFDPDTPFTGSRFE
                     ALADCHRADEIAASDIVAVSMLGVNIPAEVAVWLLSDEGRLVVSTHLVDVPANVDLWE
                     RPELIDREGELWRLWYVLGEACWPAPDRANGMGTTKISKLLAAKRPRLVPVFDSVVRD
                     VLPRVDSHWAAFSAVVSDPTRHQTILDATSSAPPHVSLLRRPDVALWMAHRRGRDPVW
                     HWRSNGAASRFSRR"
     gene            complement(1523..>2757)
                     /locus_tag="PLZ72_13140"
     CDS             complement(1523..>2757)
                     /locus_tag="PLZ72_13140"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HNA37289.1"
                     /translation="VSHSMLLNVLDRPGDGRAALHRLLTENYETDARQAEHVSRAEDL
                     FASLLDAEVVEQLDEPDDFGRTVRVTIDLQKTFALNQPLAPFALAALDLLDPDSDTFA
                     LDIVSVVESVLDDPRQVLSAQLFKAKGDAVAAMKAEGMDYDERMAALDEITWPKPLAE
                     LLEPMFAVYRERNPWVDEHPLSPKSVARDLWERAMDFGDYVRFYNLARSEGTVLRYLS
                     DVYKALVRTVPEDTKTEELVDLTEWLGELVRQVDSSLLDEWEELRNPSAESGTRTDTP
                     PIDLSPPALTANRRAFTILVRNAMFQRVEFLGTRQWNELEALDGEDGWSAQRWFDAIA
                     PYFEEHPSIGIGSDARSPDLLLIDEHPLEHPGCWSVRQILHDPADDHDWAIVALVDLA
                     ASDDAGRAVVHIIDVDCG"
     assembly_gap    2758..2857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2858..4215)
                     /locus_tag="PLZ72_13145"
     CDS             complement(<2858..4215)
                     /locus_tag="PLZ72_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HNA37290.1"
                     /translation="MTDLGQLIPISPDPDAVMDAFLGWVEAGGLTLYPHQEEALLEIV
                     SGAHVIVDTPTGSGKSLIAVAAHFAALADRRRSFYTAPIKALVSEKFFDLAATFGADE
                     VGMLTGDASINPDAPIICCTAEILANLALREGRTADVGQVVMDEFHYFADPDRGWAWQ
                     VPLLELPDAQFVLMSATLGDVSRFTDTLPDRTDRPVAVIRSATRPIPLVHEFRMTPLT
                     EVLDELLSAHQAPVYVVHFTQASAVERAQSLMSVNLLTRAEKDQIAELIGGFRFASGF
                     GRTLAKFVRHGIGVHHAGMLPKYRRLVEQLAQAGLLKVICGTDTLGVGINVPIRTVVF
                     TALAKYDGTSTRLLTAREFHQIGGRAGRAGFDTMGTVIALAPDHVIENHRAAAKAAAR
                     TAQDKKVRKIQKKKPPPGMVSWGQPTFDRLVSAPPEPLSSTFTVSHSMLLNVLDRPGD
                     GRA"
     gene            complement(4370..4864)
                     /locus_tag="PLZ72_13150"
     CDS             complement(4370..4864)
                     /locus_tag="PLZ72_13150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNA37291.1"
                     /translation="MPAPVTVSITVPQPVDEVFAFLDVMANHEPFTDHLMQDWTYSGP
                     ATGVGARARVHTRAMGVSDVVDIEVVDAEAPTRIVERNTAHKAGRVGQGTYTLAPRAD
                     GGTEVHFEYRWIKAPLMDRILAPAARSFIRKANSTAMERLAGRLAERAEADRSGPDLS
                     GDRV"
     gene            4879..5490
                     /locus_tag="PLZ72_13155"
     CDS             4879..5490
                     /locus_tag="PLZ72_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37292.1"
                     /translation="MPDGPIGTSPSDEDAGPRRRLLDAAIDRAAERGLGDLSLRALAE
                     ELGTSHRMLSHHFGSKDGLWLAIVEEVERRQRAALAELLTAIDDDTDPASVMRRWWAH
                     LTDPALLANARLFFELYGQALVGRQPAVRLLEGDIESWVESAGAGVGSELGPEVRAFL
                     RLGIAVTRGLLLDLLATGDRVGVDAAMEQWIVLAAPVVAAARD"
     gene            5570..6550
                     /locus_tag="PLZ72_13160"
     CDS             5570..6550
                     /locus_tag="PLZ72_13160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNA37293.1"
                     /translation="MSRLSYQIPNFTYPDTAPADLFPTVVAQAREAEAAGFDRVMVMD
                     HFYQLPGIGEPDEPMLECYTTLSALAQHTERVRLSALVTGNSYRNPALLAKTVTALDH
                     VSGGRATLGIGAGWFELEHDALGFEFGTFTDRFEKLEEALQIIVGMLRGERPTVSGDH
                     YSVTDALNSPAPLSSIPVMIGGNGERKTLRLVARYADESNLMGMGGDVDVARKLAVLA
                     QYCEHEGRDPSEIAVTMLVGCVVAPSAKELDSDLRAMAAAKGWGDGVIDMVKQILICG
                     DADTVGERLGELAASGVTGLVCNLPVNGHVPGRISLMAEVADRALTAAGR"
     gene            6560..7060
                     /gene="msrA"
                     /locus_tag="PLZ72_13165"
     CDS             6560..7060
                     /gene="msrA"
                     /locus_tag="PLZ72_13165"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNA37294.1"
                     /translation="MTTERAILANGCFWGSQELLRHLPGVISTRTGYSGDPATPNATY
                     RRHGDHAEAVEVVFDPDELSYRRLLEFFFQIHDPTTRDRQGNDIGRSYRSAIFHIDDR
                     QRQIALDTIADVDRSGRWPGPVVTEVEPASDFWEAEPEHQDYLQRIPNGYTCHWVRPD
                     WTLPTD"
BASE COUNT         1087 a         2346 c         2470 g         1064 t
ORIGIN      
        1 ccgatgggcg gcctggcgaa ccaagcagaa gctcgaggac gaggcgcccg agcgcatcga
       61 cgacctcatc gcctccctgg cggatttcgc cgacccgatc ctcgacggca ccgccgagcc
      121 cctcatctgg gacccgacct cccgctcctg gcagcagtag ccgggcgcgc ccccacggct
      181 gaggggtcga ggaccggcag gtgtggtacc agcgccgaaa ggctcaggtc gccagccgac
      241 gaccgaccag cgctgccatt cgatccgcca catccgcggc gtggatccag tccaccacct
      301 cgaagtcggc gatccgcagg gtcggtcgcc acccgtggac ggatggcagc aggtctgcga
      361 ggccatcgcg aaacgctcgc tgcaggtgcc gtccgcctgg gtcggggaac aggggcggac
      421 agggccgggc gaagccgcca cccggaaggc gcttggcggc ccgtgctcgc gcagcccact
      481 ccggagcatc gaaacccgtc ggcaggtcgt cggggaagtg gtcgagcacg accgcgcggg
      541 gcggcatgaa gtgctgcttc tcgtcgatct cgacgagtcg acgggttccg tcggggtggg
      601 tgaggaccgc gtcgatggtc cgacgctctc tcggtggcat cgcttccggc aggtcgcgac
      661 cggtgagcgc cgtgtagatc tcccgcacga gaggccactg cgtgccgcag tcgtctcggc
      721 cgggtcggag cagccaagcg ggagtcggac cgggtgcgag ctcaccgtcg gctacctgcg
      781 agagaagcgc gatgcagcgc cgttggacct ccagtgccac acgggatcac gcccccgtcg
      841 gtgtgccatc cacagcgcca cgtcggggcg tcggagcagg ctgacgtggg gcggagccga
      901 cgacgtggca tcgaggatcg tctggtggcg agtcgggtcc gacacgaccg ccgagaacgc
      961 tgcccagtgg gaatccaccc gggggagcac gtcgcgcacg accgagtcga acacgggcac
     1021 caggcgagga cgcttggcgg cgagcagctt cgagatcttg gtggtgccca tgccgttcgc
     1081 tcggtctggg gcaggccaac acgcctcgcc gagcacgtac cagagtcgcc agagctcgcc
     1141 ctcccgatcg atgagctccg gacgctccca caggtcgacg ttcgccggga cgtcgaccag
     1201 atgagtcgag acgacgagtc gtccctcgtc gctgagtagc cacacggcga cctcggctgg
     1261 gatgttcacg cccagcatcg agactgcgac gatgtccgac gcagcgatct cgtcagcgcg
     1321 gtggcaatcg gcgagcgcct cgaaccgcga gccggtgaac ggcgtgtcgg gatcgaagta
     1381 ggtcgcgacg tcgctgcggg ccctgcttcc gccgtccgcc gtcaccacgg ccatgacggc
     1441 atctacgaac tccccgtagt cgtcctgcat cagctcggac cgtctggcgc gccgcgtcca
     1501 ggctgcgttc tgcctgtccc gcctaaccgc agtcgacgtc gatgatgtgc acgacggctc
     1561 ggccggcgtc gtcgctggcg gcgaggtcga cgagagcgac gatggcccag tcgtggtcat
     1621 cggcgggatc gtggaggatc tgacggaccg accagcagcc ggggtgctcg agcggatgct
     1681 cgtcgatgag gagcaggtcg gggcttcggg cgtcggaacc gatgccgatg ctcgggtgct
     1741 cctcgaagta cggagcgatc gcgtcgaacc atcgctgggc gctccacccg tcttcgccgt
     1801 cgagcgcctc cagctcgttc cactgacggg tgccgaggaa ctccacccgt tggaacatgg
     1861 cgttgcgaac gaggatggtg aacgcccggc ggttggccgt cagggccggc ggtgaaaggt
     1921 cgatcggcgg cgtgtcggtc cgggtcccgg attctgcgct cgggttgcgc agctcctccc
     1981 attcgtcgag aaggctggag tccacctggc ggaccagctc cccgagccac tcggtgagat
     2041 cgacgagctc ctcggtcttg gtgtcctcgg ggacagtccg caccagcgcc ttgtagacgt
     2101 ccgacaggta gcgcagcacg gtgccctcgg aacgggccag gttgtagaag cggacgtagt
     2161 cgccgaagtc catggcgcgc tcccacaggt cccgggcgac cgacttgggc gacagcgggt
     2221 gctcgtccac ccaggggttg cgctcgcggt agacggcgaa catcggctcg agcagctccg
     2281 cgagcggctt gggccacgtg atctcgtcga gcgcggccat ccgctcgtcg tagtccatgc
     2341 cctcggcctt catcgcggcc accgcatcgc ccttggcctt gaacagctgg gcgctgagga
     2401 cctggcgggg gtcgtcgagc acggattcga ccaccgacac gatgtcgagc gcgaacgtgt
     2461 cggagtcggg gtcgaggagg tccaacgcgg caagagcgaa cggtgccagc ggttggttga
     2521 gggcgaaggt cttctggagg tcgatggtga cccggaccgt acgaccgaag tcgtcgggtt
     2581 cgtccagctg ctccaccacc tcggcgtcga ggagcgaggc gaagaggtcc tcggcgcggg
     2641 agacgtgctc ggcctgcctg gcgtcggtct cgtagttctc ggtgagcagt cggtggagtg
     2701 cagcccgccc gtcgccgggt cggtcgagca cgttgagcag catcgagtgg ctcaccgnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc cgcccgtcgc cgggtcggtc
     2881 gagcacgttg agcagcatcg agtggctcac cgtgaacgtg gaagacaacg gctccggagg
     2941 ggcgctcacc aggcgatcga aggtcggctg gccccacgac accatccccg gcggcggctt
     3001 cttcttctgg atcttgcgga ccttcttgtc ctgcgcggtc cgagccgccg ccttcgccgc
     3061 agccctgtgg ttctcgatga cgtggtccgg ggcgagggcg atgacggtgc ccatcgtgtc
     3121 gaagcccgcc cgcccggcgc ggccgccgat ctggtggaac tcccgcgcgg tgaggaggcg
     3181 ggtcgacgtt ccgtcgtact tggccagggc ggtgaagacc acggtgcgga tgggcacgtt
     3241 gatgccgacg ccgagggtgt cggtcccgca gatgaccttc agcagcccgg cctgggccag
     3301 ctgctccacc agacgtcggt acttgggcaa catccccgcg tggtgcacgc cgatgccgtg
     3361 gcgtacgaac ttggcgagcg tcctgccgaa ccccgacgcg aagcggaacc cgccgatgag
     3421 ctcggcgatc tggtccttct cggcgcgggt gagcaggttg acgctcatca gcgactgcgc
     3481 ccgctcgacc gccgatgcct gggtgaagtg aacgacgtac acgggggcct ggtgcgcgga
     3541 cagcagctcg tcgagcacct cggtgagcgg cgtcatccgg aactcgtgga cgagggggat
     3601 gggacgggtg gccgaccgga tgaccgcaac gggccggtcg gtgcgatcgg ggagcgtgtc
     3661 ggtgaaccgg ctcacatcgc cgagcgtggc ggacatcagg acgaactgag cgtcggggag
     3721 ctcgagtagc gggacctgcc acgcccagcc ccgatcggga tcggcgaagt agtggaactc
     3781 gtccatgacg acctggccga cgtccgcggt gcgaccctcg cgcagcgcga ggttggccag
     3841 gatctcggcc gtgcagcaga tgatcggggc atcggggttg atcgacgcgt caccggtcag
     3901 catgccgacc tcgtcggccc cgaacgtggc tgccaggtcg aagaacttct ccgacaccaa
     3961 ggccttgatc ggtgcggtgt agaagctccg gcgccggtcg gccagggcgg cgaagtgcgc
     4021 ggcgaccgcg atgaggctct tgccggaccc ggtgggcgtg tcgacgatga cgtgggcccc
     4081 ggagacgatc tcgaggagcg cttcctcctg gtgcggatac agggtgagcc cgccggcctc
     4141 gacccagccg aggaacgcgt ccatgaccgc gtcgggatcg ggcgagatcg ggatcagctg
     4201 gccgaggtcg gtcaccccgg cagcgtagga ggcggggcct ccaggatcgt ctccgccgca
     4261 gccgggccga cccgcccggc cgccgatcgt ccgcccgact gctcgtccga gtgtccgtcg
     4321 cctgcggggg ccgcgccgga gcggtgggga gctcgatcgg tcatcgacgt cagacgcggt
     4381 cccccgagag atctgggccg gaacggtccg cctcagccct ctcggcgagt cggcccgcca
     4441 gtcgctccat ggcggtggag ttggccttgc ggatgaacga cctcgcggcc ggcgccagga
     4501 tccggtccat gagcggagcc ttgatccagc ggtactcgaa gtgcacctct gtcccaccgt
     4561 cggcacgggg cgccagcgtg taggtgccct ggccgacgcg tccggccttg tgtgccgtgt
     4621 tgcgctcgac gattctcgtc ggtgcctcgg cgtcgaccac ctcgatgtcg accacgtcgg
     4681 acacccccat cgcgcgggtg tgcacccggg cacgtgcccc gacgccggtc gccggtcccg
     4741 agtaggtcca gtcctgcatc aggtggtcgg tgaacggctc gtggttggcc atcacgtcga
     4801 ggaatgcgaa cacctcgtcg acgggctggg gaacggtgat ggagacggtg acgggagcag
     4861 gcatgtacca tatggtacat gcctgatgga ccaattggta catcaccgtc cgacgaggac
     4921 gccggaccgc gtcgtcgcct tctcgatgcc gcgatcgatc gcgcggccga gcgcggactc
     4981 ggtgacctca gcctgcgtgc cctcgccgag gagctcggca ccagccaccg gatgctcagc
     5041 catcacttcg gatcgaagga cgggctctgg ttggccatcg tcgaggaggt ggagcggcgc
     5101 cagcgagcgg ccctggccga gctcctcact gcgatcgacg acgacaccga ccccgcgagc
     5161 gtgatgcgtc gatggtgggc gcatctcacc gatccggcct tgctcgccaa cgcccggctc
     5221 ttcttcgagc tgtacggaca ggcgctggtg ggccgccagc cggcggtgcg gctcctcgaa
     5281 ggtgacatcg agagctgggt ggagtccgcc ggggccggcg tcgggagcga gctcggtcct
     5341 gaggtccggg cgttcctccg attgggaatc gccgtcaccc ggggcctcct gctcgacctg
     5401 ctcgccaccg gagaccgcgt cggggtcgac gcagcaatgg agcagtggat cgtcctggcc
     5461 gctccggtgg tagccgccgc ccgcgactga ggctcgatca gacgcctcgc ccgcacgcac
     5521 ggtcgatcga cagccgcgcc aaactcgtgg cgaacccagg gaggcgacct tgtcacgact
     5581 gagctaccag atccccaact tcacctatcc cgacacggcc cccgcagacc tgttccccac
     5641 ggtcgtggcc caggcacgcg aggccgaggc cgcagggttc gaccgggtca tggtgatgga
     5701 ccacttctac caactgcccg gaatcgggga gcccgacgaa ccgatgctcg agtgctacac
     5761 gacgctctcc gcgttggctc aacacaccga gcgggtgcgg ctctcggccc tcgtgaccgg
     5821 gaacagctac cggaacccgg ccctgttggc caagacggtg accgctctcg accacgtgtc
     5881 gggcggccgg gcgacgctcg gcatcggcgc gggctggttc gagctcgaac acgatgcgct
     5941 cggcttcgag ttcggcacct tcaccgaccg gttcgagaag ctcgaggagg cgctgcagat
     6001 catcgtcggg atgctgcggg gcgaacgacc cacggtcagc ggcgaccact actcggtcac
     6061 cgacgctctc aactctccgg caccgctctc gtcgatcccc gtcatgatcg gaggcaacgg
     6121 ggagcgcaag acgctgcgcc tcgtggcccg ctatgcggac gagtcgaacc tgatgggcat
     6181 gggaggtgat gtggacgtcg cccgcaagct cgcggtgttg gcgcagtact gcgagcacga
     6241 ggggcgcgac ccctccgaga tcgcggtcac gatgctggtc ggctgcgtcg tggctccgag
     6301 cgccaaggag ctcgactcgg acctccgggc catggcggcg gccaagggat ggggcgacgg
     6361 ggtgatcgac atggtcaagc agatcctcat ctgcggcgac gccgacaccg tcggtgagcg
     6421 tctcggcgag ctcgctgcct cgggtgtgac gggcttggtc tgcaacctgc cggtgaacgg
     6481 ccacgttccg ggccgcatct cgctgatggc cgaggtcgcg gaccgggcct tgaccgcggc
     6541 cgggcgctga ggagcgacga tgacgaccga acgggcgatc ctggccaacg gctgcttctg
     6601 gggctcccag gaactgctgc ggcaccttcc aggtgtcatc tccacccgca ccggatattc
     6661 gggcgatccg gcgacaccga acgccaccta ccgccgtcac ggcgaccacg cggaggcggt
     6721 cgaggtggtc ttcgaccccg acgagctcag ctaccgccgc ctgctcgagt tcttcttcca
     6781 gatccacgac ccgaccacgc gggaccggca gggcaacgac atcggtcgca gctaccgatc
     6841 cgccatcttc cacatcgacg accggcagcg gcagatcgcc ctggacacca tcgccgacgt
     6901 cgacagatcg ggccgctggc cgggcccggt ggtcaccgag gtcgagcccg ccagcgactt
     6961 ctgggaggcg gagcccgagc accaggacta tctgcagcgg atcccgaacg ggtacacctg
     7021 ccactgggtc cgacctgact ggaccctgcc caccgactga cgcagcg
//
LOCUS       DAOQOD010000164         7010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4104_length_7010_cov_10.661970, whole genome shotgun sequence.
ACCESSION   DAOQOD010000164 DAOQOD010000000
VERSION     DAOQOD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_7010_cov_10.661970"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLZ72_13170"
     CDS             <1..818
                     /locus_tag="PLZ72_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37295.1"
                     /translation="ALADELHRALGSRGLACSRVLVVAGTDAGDRIERLWRADGALSA
                     AAIAQRVRWQLEGWLGSGRTRSRAHGGVDRLELVPDEVRPDDGLQLGFWGGTSEAGER
                     ALRALARVQGLVGADRVLVPEWQGGRAPGERYRLVPLDAVDLSARTTTGPEPWPGSLP
                     APSPAVVWPEPRPVDVLDAAGRRVGVGGRGLISSPPTSVRLNPGAGPAGAAGVASVRA
                     WAGPWCVDERWWDAVGHRRRARLQVVLAGPDGHETAHLLTLEDGRWWLEATYD"
     gene            968..1687
                     /locus_tag="PLZ72_13175"
     CDS             968..1687
                     /locus_tag="PLZ72_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNA37296.1"
                     /translation="MTAAEETASSDPQGRVLIVEDEVALADGVARGLIAEGFEVEVVH
                     DGVSGLERARAEDLDAIVLDIMLPGLNGYRVCRTLREEGIGTPILMLTAKQGEYDEAE
                     ALDTGADDFLSKPFSFVVLIARLRALLRRSADGRAQPLVIGDLVLDPLARTCRRGDTD
                     ISLTTREYELLEALMRKPGQVVAKQDLLEKVWGSDFEGDPNVVEVYVGYLRRKIDRPF
                     DRNDIETVRGVGYRIGSGADG"
     assembly_gap    1793..1892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1893..3020
                     /locus_tag="PLZ72_13180"
     CDS             <1893..3020
                     /locus_tag="PLZ72_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNA37297.1"
                     /translation="VISTTYFYLDGQAFSQMKVRGVDSDGRLVLFGRVGPALPDPTDA
                     IESTRSVPTKWGDLTIHAVSPLAEIERSVNALRAALWIGLPLLVLAAGAMTWVITGQA
                     LAPVGVMTRRVRELSSTNLDARVPVPPTEDEIALLAVTMNDMLDRLEKASVKQRQFVS
                     DASHELRSPVASIRTQIETALRYPDDVDWPSVARTVLAEDDRLDHLVGNLLAMARLEE
                     GRFGPRSDVDIDELVMAQRARMSDVSLDLSGVSAGRVWGNSDELTSVVRNLLDNAARH
                     CRSRVVVTVGEEGPWVVLTVADDGDGVAPEDRQQVFARFARLQEGRARDSGGTGLGLA
                     LTKRIVEHHGGRIHVEDGEQGGARFVVSLPSASWTGAPDAT"
     gene            complement(3046..5298)
                     /locus_tag="PLZ72_13185"
     CDS             complement(3046..5298)
                     /locus_tag="PLZ72_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNA37298.1"
                     /translation="MPADPPDDHLFDVVPIAPDKVSTAPPRATPTDLSTVLLDHLDVG
                     VWAIAPDGTVLAVNRAAAATIGEQPDTLIGRRFTDLVDPPLSDDGWHLLLDRVDDDRH
                     HRVRLALGTRHGHSVTADLEIGRHRSVQSDVVMVTVRTAAGPGSADPADAPDAAAGSP
                     EVALTDPVDERLRRTIAELPDGIALVDASGRIDHVNDTFCRLVGRSRAQIRHRSVFDP
                     PWMFRDEGDRATTPEESAAVICLRSGQAAVGPVFSVALSRTERIWSRMSAHPVPGGAV
                     VVLEDRTTEVSLRAQIAAVSSTDELTGLGSRRSLHAQLAGLLARDADEQADGPHRVGV
                     LHIDLDGFRTVNDTFGPAIGDRVLVEVADRVRDLADRSPDQAQVSIGRVGVDEFMVSV
                     DGFGPSLAFDMELRRLAEEVQRRLQSPIAVDGLDIRLTASIGVARSPVDATDAERLVV
                     AAEQALAAGRREGRNRLRFYKPELDAATRTGLALDRDLRRAAAARQLEVHYQPIIDLR
                     TGSIAMAEALVRWHHPVEGPIPPSVFIPTAEATGAISAVSDLVISTVARDIAGWTRDR
                     ILPSRARIAVNISPTEFEQRDFTDRLAATLDQAGVSPSQLELEITESLLVQDLRAAAS
                     RLEQLDDLGFLIALDDFGTGYSSLSYLHTLPFHTLKIDRAFVGDLREGRSGSITRAIL
                     TLAHNLGIVAVAEGVETDGQRSFLHEAGCDLVQGFLYAPPMPRAAFERFLVEHGGVVP
                     GIDDPLTIGL"
     gene            complement(5427..>7010)
                     /locus_tag="PLZ72_13190"
     CDS             complement(5427..>7010)
                     /locus_tag="PLZ72_13190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNA37299.1"
                     /translation="GRAGYYAMLRFSEDTQDPGRGAEMMKVQERGTAIAAKLVFLELE
                     WAALPDERVDELLADPVLDFCAHHLRSARRYRDHLLSEPEEVLLTEKSTSGAAAWVRL
                     FDELTSAITVELPEQGTVSLEQGLSLLQHPDRAVRQQAAAAVTAGLEPGLRTRAFVFN
                     TLLLDKSTDDRLRSYPSWVSSRNLSNEATDDSVQALVDAVVSRYDIAQRWYRLKAQVL
                     GLDRLADYDRMASVAEDESEIGWAEATALVHDAYASFSPELAGIVQRFYDGGWIDAPV
                     RPAKRPGAFCAYTVPSHHPYLLLNWTSRNRDVLTLAHELGHGLHAYLSRGQGTFHQST
                     PLTLAETASVFGETVTNNALLARIDDPNERFALLAATLEDSIATVFRQVAMNRFEDAV
                     HTARREEGELSVERFGELWATTQEAMVGDSVDITDGYRTWWSYIPHFIGTPGYVYAYA
                     YGQLLALSVYARYQEQGASFVPAYLELLSAGGSMEPAALGRIVGCDLEDPGFWDAGLA
                     IVDGQLRAAEDAARAAGRL"
BASE COUNT          937 a         2363 c         2648 g          962 t
ORIGIN      
        1 aggccctggc cgacgagctg caccgggcgc tgggctcgcg cgggttggcc tgcagccgcg
       61 tgctggtggt ggccgggacc gacgccggcg accgcatcga acggctctgg cgggccgacg
      121 gcgccctgtc ggccgcggcc atcgcccaac gggtccgttg gcagctcgag gggtggctgg
      181 ggtcgggccg gacccgcagc cgggcccacg gcggtgtgga ccgcctggag ctggtgcccg
      241 acgaggtccg ccccgacgac ggcctccagc tgggcttctg gggtgggacc agcgaagcgg
      301 gcgagcgggc cctgcgtgcg ttggcccggg tgcaggggct ggtcggagcg gatcgggtgc
      361 tggtccccga gtggcagggg ggccgggctc cgggcgagcg ctaccggctg gtgcccctcg
      421 acgcggtgga cctgtcggcg aggacgacca cgggtccgga gccgtggccg ggatcgctgc
      481 cggcgccgtc acccgcagtg gtgtggcccg agccccgccc cgtggatgtc ctcgatgccg
      541 ccggtcgacg ggtgggtgtc ggcgggcggg ggctgatctc ctcgcctccg acatcggtgc
      601 gcctgaaccc cggtgccggg cccgccggag ctgccggggt ggcgtcggtg cgggcgtggg
      661 cgggaccgtg gtgcgtcgac gagcggtggt gggatgcggt cggccaccgt cgtcgggccc
      721 gtctgcaggt ggtgctcgcg gggcccgacg gacatgagac ggcccacctg ctcaccctgg
      781 aggacggccg ctggtggctc gaagccacct acgactgatc ccaccgaacc accgcggaac
      841 ggggtctcag ctcggcgcag tccgggcacc ggttcggcac cggaacctga gagactgggc
      901 gaggtgtcga cacgctcgat gcccgaccgg atcggccggt cgcgacccga catcgaggag
      961 cacgcccgtg accgcagccg aggagaccgc gtcgagcgac ccgcagggcc gggtcctgat
     1021 cgtggaggac gaggtggcgc tggccgacgg cgtcgcccgc ggcctgatcg ccgagggctt
     1081 cgaggtcgag gtcgtccacg acggcgtgag cggcctggag cgtgcccgcg ccgaggacct
     1141 cgacgccatc gtgttggaca tcatgttgcc gggcctgaac ggctaccggg tgtgccgcac
     1201 cctgcgcgag gaggggatcg ggaccccgat cctcatgctg accgccaagc agggcgagta
     1261 cgacgaggcc gaggcgctcg acaccggggc cgacgacttc ctgtccaagc cgttctcgtt
     1321 cgtggtcctg atcgcccggc tccgggcgct gctgcgccgc tcggccgacg ggcgggccca
     1381 gcccctggtc atcggcgacc tcgtgctcga cccgctggcg cgcacctgcc gtcgcggcga
     1441 caccgacatc tcgctgacca cccgcgagta cgagctgctc gaggcgctga tgcgcaagcc
     1501 cgggcaggtc gtcgccaagc aggacctcct cgagaaggtc tggggcagcg acttcgaggg
     1561 ggaccccaac gtcgtcgagg tctacgtggg gtacctgcgc cgcaagatcg accggccgtt
     1621 cgaccgcaac gacatcgaga cggtgcgcgg cgtcggctac cgcatcggca gcggagccga
     1681 tggctgatcc ggtcggtgtc gacggcgtcg gtcccgccgt ctccgatgat gccgcagccg
     1741 ggtacgggcc gccggatccg tccggaaccg aggcgccccg tgtgcggagg cgnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgatctc gacgacgtac ttctacctcg
     1921 acggccaggc gttctcgcag atgaaggtgc gcggcgtcga cagcgacggt cgactggtgc
     1981 tgttcggtcg cgtcggcccg gccctgccgg acccgaccga cgccatcgag tccacccgca
     2041 gcgtgcctac caagtggggt gacctcacca tccatgccgt gtcaccgctg gccgagatcg
     2101 aacggtcggt gaacgcgctg cgcgccgcgc tgtggatcgg tctgccgttg ctggtgctcg
     2161 ctgccggcgc gatgacgtgg gtcatcaccg gacaggccct ggcgcctgtc ggggtcatga
     2221 cccgtcgggt gcgagagctg tcgagcacga acctcgacgc ccgggtcccg gtgccgccga
     2281 ccgaggacga gatcgcactg ctggcggtca ccatgaacga catgctcgac cggctggaga
     2341 aggcgtcggt caagcagcgc cagttcgtct ccgacgccag ccacgagctg cgctccccgg
     2401 tggcgtcgat ccgcacccag atcgagacgg ccctgcgcta ccccgacgac gtcgactggc
     2461 cgtcggtggc gcggaccgtg ctcgccgagg acgaccgcct cgatcacctg gtcggcaacc
     2521 tgctcgccat ggcccgactg gaggagggcc gcttcggtcc gcgctccgac gtcgacatcg
     2581 acgagctagt catggcgcag cgcgcccgga tgagcgacgt gtcgctcgac ctgtcggggg
     2641 tgtcggccgg ccgggtgtgg ggcaacagcg acgagctgac cagcgtggtg cgcaaccttc
     2701 tcgacaacgc cgcccgccac tgtcgcagcc gtgtggtggt caccgtcggg gaggaggggc
     2761 cgtgggtcgt gctgaccgtc gccgacgacg gcgacggcgt cgccccggag gaccgccagc
     2821 aggtgttcgc ccgcttcgcc cgcctccagg agggccgggc ccgagactcg ggcgggaccg
     2881 gtctgggact ggcgctgacc aagcgcatcg tcgagcacca cggcggtcgc atccacgtcg
     2941 aggacgggga gcagggcgga gcgcgcttcg tggtctcgct cccgtcggcc agctggaccg
     3001 gggcgcccga cgccacctga tccggttccc gcccggcagg tccggtcaga ggccgatcgt
     3061 gagcgggtcg tcgatgccgg ggacgacgcc gccgtgttcg accaggaacc gttcgaacgc
     3121 ggcgcggggc atgggcggcg cgtacaggaa accctgcacc aggtcgcacc ccgcctcgtg
     3181 gaggaagctg cgctggccgt cggtctcgac cccttcggcc accgcgacga tgccgaggtt
     3241 gtgggccagg gtcaggatcg cccgggtgat cgaccccgag cgcccctcgc gcaggtcgcc
     3301 gacgaacgcc cggtcgatct tgagggtgtg gaacggcagg gtgtgcaggt acgacaacga
     3361 cgagtagccg gtgccgaagt cgtccagggc gatgaggaag cccaggtcgt cgagctgctc
     3421 caggcggctg gccgcggccc gcaggtcctg gaccagcagg ctctcggtga tctccagctc
     3481 gagctgcgac ggcgagaccc cggcctggtc gagcgtggcg gccagacggt cggtgaagtc
     3541 gcgctgctcg aactccgtcg gcgagatgtt gaccgcgatg cgggcgcggc tcggcaggat
     3601 gcggtcccgg gtccagccgg cgatgtcgcg ggcgacggtg gagatcacca ggtcgctcac
     3661 cgcgctgatc gcgccggttg cctcggctgt ggggatgaag accgacggcg ggatcggccc
     3721 ctcgaccgga tggtgccaac gcaccagcgc ctcggccatg gcgatggacc cggtgcgcag
     3781 gtcgatgatc ggctggtagt gcacctcgag ctggcgggcc gcggcggccc gacgcaggtc
     3841 gcggtcgaga gcgaggccgg tgcgcgtcgc ggcgtcgagc tcgggcttgt agaaccgcag
     3901 ccggttgcgc ccttcacggc ggcccgccgc gagcgcctgc tcggcggcga cgaccaggcg
     3961 ctcggcgtcg gtggcgtcga ccggcgatcg ggccacgccg atcgaggcgg tgaggcggat
     4021 gtccaacccg tcgacggcga tcggggactg cagccgacgc tggacctctt cggccagccg
     4081 gcgcagctcc atgtcgaacg ccaggctggg tccgaacccg tcgacgctca ccatgaactc
     4141 gtcgacgccg acccggccga tgctcacctg cgcctggtcg ggggagcggt cggccaggtc
     4201 ccgcacccgg tccgcgacct cgacgagcac ccggtcgccg atggcgggtc cgaaggtgtc
     4261 gttgacggtg cggaagccgt ccaggtcgat gtgcagcaca cccacgcggt gcggcccgtc
     4321 ggcttgctcg tcagcgtccc gggcgagcag gccggccagc tgcgcatgga gcgagcgtcg
     4381 ggatccgagg ccggtcagct cgtcggtgct gctcaccgcc gcgatctgcg cacgcagcga
     4441 cacctcggtc gtgcggtcct cgaggacgac cacggcgccg ccggggacgg ggtgtgcgga
     4501 catgcgcgac cagatccgct cggtccggct gagcgcgacg gagaacaccg gtccgaccgc
     4561 agcctgaccg ctgcgcaggc agatcaccgc cgcggactcc tcgggcgtgg tggcccggtc
     4621 cccttcgtcg cggaacatcc acggtgggtc gaacacggag cggtgacgga tctgggcccg
     4681 ggaccgaccg acgagccggc agaaggtgtc gttgacgtgg tcgatccgcc ccgaggcgtc
     4741 gacgagggcg atgccgtcgg ggagctcggc gatggtgcgt cgcaaccgct cgtcgacggg
     4801 atcggtcaac gcgacctctg gtgacccggc cgccgcatcc ggggcatccg ccggatccgc
     4861 gctccccggt ccggcggcgg tgcgcaccgt caccatcacc acgtcgctct ggaccgaccg
     4921 gtggcgtccg atctctaggt ctgcggtcac cgagtggccg tgacgggtgc ccagggcgag
     4981 ccggacccgg tggtggcggt cgtcgtcgac ccggtcgagc agcaggtgcc agccgtcgtc
     5041 gctcagcggc gggtcgacca ggtcggtgaa gcgtcgcccg atcagcgtgt cgggctgctc
     5101 gccgatggtg gcggccgcgg cccggttgac cgcgaggacg gttccgtcgg gagcgatggc
     5161 ccacacgccc acgtcgaggt ggtcgagcag cacggtggac aggtcggtgg gggtggcgcg
     5221 gggaggagcg gtggacacct tgtcgggcgc gatcgggacg acgtcgaaca ggtggtcgtc
     5281 aggcgggtcg gcgggcacgc gggagggctc ctgtggcggt gacccggccc ctccggatca
     5341 ccgaacggtc tcctacccta cggagccgtt ccgcggcact ggtggacccc ggaacccccc
     5401 gcggccgtgc ctgtcagagg cgcctgtcag aggcgcccgg cggcgcgagc ggcgtcctcg
     5461 gcggcgcgga gctgaccgtc gacgatggcg agccccgcgt cccagaagcc cgggtcctcg
     5521 aggtcgcatc cgacgatgcg gccgagcgcg gcgggctcca tcgaaccgcc ggccgacagc
     5581 agctccaggt aggccggcac gaacgacgcg ccctgctcct ggtagcgggc gtacaccgac
     5641 agcgcgagca gctggccgta ggcgtaggcg tagacgtagc cgggggtgcc gatgaagtgc
     5701 gggatgtagc tccaccaggt ccggtatccg tcggtgatgt cgaccgagtc gccgaccatg
     5761 gcctcctgcg tggtggccca cagctcgccg aagcgctcga ccgacagctc gccctcctct
     5821 cggcgggcgg tgtgcacggc gtcctcgaag cggttcatcg ccacctggcg gaacaccgtc
     5881 gcgatcgagt cctcgagcgt cgccgcgagc agggcgaatc gctcgttcgg gtcgtcgatg
     5941 cgggcgagca gggcgttgtt ggtgacggtc tcgccgaaca ccgacgcggt ctccgcgagc
     6001 gtcagcggtg tcgactggtg gaacgtgccc tgaccgcggg acaggtaggc gtgcaggccg
     6061 tggccgagct cgtgggccag cgtcagcacg tcgcggttgc ggctggtcca gttgagcagc
     6121 aggtacgggt ggtgcgacgg cacggtgtag gcgcagaacg ctccgggacg cttggccggc
     6181 cggaccgggg cgtcgatcca gccgccgtcg tagaagcgct gcacgatccc ggcgagctcg
     6241 ggcgagaagc tggcgtaggc gtcgtggacc agggcggtgg cctcggccca gccgatctcg
     6301 gattcgtcct cggccaccga cgccatgcgg tcgtagtcgg ccaggcggtc gagtcccagc
     6361 acctgggcct tgaggcggta ccagcgctgc gcgatgtcgt agcgcgacac gaccgcgtcg
     6421 acgagcgcct gcaccgagtc gtcggtggcc tcgttggaca ggttcctcga cgacacccac
     6481 gacgggtagc tgcggaggcg gtcgtcggtc gacttgtcga gcagcagcgt gttgaacacg
     6541 aaggcccggg tccgcaggcc gggctccaac ccggcggtca ccgcggcggc ggcctgctgg
     6601 cggacggccc ggtcggggtg ctgcaacagc gacagcccct gctccaggct gacggtgccc
     6661 tgttcgggca gctcgacggt gatggccgag gtcagctcgt cgaacagccg gacccaggcg
     6721 gcagcgcccg aggtcgactt ctcggtcagc agcacctctt cgggctcgct gagcaggtgg
     6781 tcgcggtagc ggcgggccga gcgcaggtgg tgcgcgcaga agtcgaggac ggggtcggcg
     6841 aggagctcgt cgacccgctc gtcgggcagg gcggcccact cgagctcgag gaacaccagc
     6901 ttggcggcga tggcggtgcc cctctcctgc accttcatca tctcggcccc gcgcccgggg
     6961 tcctgggtgt cctccgagaa gcgcagcatg gcgtagtagc cggcgcggcc
//
LOCUS       DAOQOD010000165         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4254_length_6834_cov_11.038944, whole genome shotgun sequence.
ACCESSION   DAOQOD010000165 DAOQOD010000000
VERSION     DAOQOD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_6834_cov_11.038944"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="PLZ72_13195"
     CDS             complement(<1..2057)
                     /locus_tag="PLZ72_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37300.1"
                     /translation="MSPARSFAGQDALRSVARTIGPAAVVVAIQLIWFPMPAGGVLSG
                     VVLGLLGSLSALGLALIWRANRVVNFAQGDLGAFPATLAVLLITLAGWPWLLGISAGL
                     GAAVLTGLLADVLVIRRFHRSPRLILTVATIGLSQVLAFAALALPDLWGEGPAVRSLP
                     TPFELRVELGGVVFDANDVIALVAAPVLLAVVASVLRFTDTGVAVRASAERAERAALL
                     GIPVRRLEAQVWTGAAVLSFFSVVLTAGVASLPFGIGLGLSVLLRALAALVVGRMTHL
                     VAITATAVALGILESGIRWNTGESWLVTPFLAGLIVLSLLLERRGASRAEQDQTSSWR
                     TSGDVRPTPWSLVRLPEVRAVRLAGGLLLALVALGLPQVIGTNGQIKAGVVLVFATIG
                     TSIVVLTGWAGQVSLGQMAFVGAGGAVGAWAMVEQGWDPLVAMALAGVIGAAVAVAVG
                     LPALRLRGMYLAVTTLALSIAASDWIFSNKVADWIPRGSFPRPALLGRIALDTPLRIY
                     YFALAVLALSVLALGGIRNSRTGRVLIALRDNEAGATVYGISTIRAKLTAFAVSGTVA
                     AVAGVVLVISQGAFRDVTYGPDQSLDVFVATVIGGLGSLAGAVIGAVYQRGAQWLLPA
                     PWSFLATGLGVLVVLLSMPDGLGGLAFRGRDRFLAWAARRHGVANLAIDRTHQDPAAD
                     DAVL"
     gene            complement(2264..3934)
                     /locus_tag="PLZ72_13200"
     CDS             complement(2264..3934)
                     /locus_tag="PLZ72_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HNA37301.1"
                     /translation="MLSNELSLISRIESVAEHGGGKVTFVDGDDHTTLTWGQLHAEAR
                     AVAAALQARGVGPGDHVALLGPTTRRLVTAIQAIWLTGGALVTMPLPMRMGALDQFIE
                     QTRTRIRRSDSKVVVIDSQLAAFVEPVEGDPPFIGLDELFVADGPTAEDYVRPADDPS
                     SLAILQFTSGSTSEPKGVMLPHQNICHNLDGAWISAELSHDEVIVSWLPLYHDMGLVG
                     LLTIPMTRDTNLVQGAPQDFLAKPIRWMRWLSQFHGTASAGPNFSYALATRALKRTDE
                     DLDLSQVRMFLNGAEPIDAPTFRRFLEAGERFGLEPGVAFPAFGMAEVCIAGCFPTPG
                     KGLITDIVDRAALERDQKAVPVDSDTEGAVELALLGRPVPGLEISIRDPRTGEECGDR
                     RVGELRIAGNSLTTGYYNQPDATAELITDGWLNTGDLAYTVGGELVICGRIKDVIIVG
                     GRNIYPQDIEKVVGEVEGVRTGNVIAFGIEGRQGAQNVIVVAEAKAAGDELEREITRV
                     ATDAVGLPPKEVVLVQPGSVPKTSSGKLQRSACRLQYLADELDRLERP"
     gene            complement(4039..6612)
                     /gene="leuS"
                     /locus_tag="PLZ72_13205"
     CDS             complement(4039..6612)
                     /gene="leuS"
                     /locus_tag="PLZ72_13205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNA37302.1"
                     /translation="MSEAASAGPSGAPDAPTIDPYRPQEIEPHWQAYWRERGTYEVDN
                     DDPRPRSYVLCMYPYPSGAAHMGHVRNYTFGDLLTRYRTMQGEAVLSPMGFDSFGLPA
                     ENAAINTGVHPRTFTDERIEMLRSSITRIGGVYDWRREVRSHSPEYIHWSQWIFLKLF
                     EAGLAYRKAAPVNWCPGCQTVLANEQVVDGRCERSGDAVERRDLEQWYLRITDYAQQL
                     LDDLELVQWPERVVTQQRNWIGRSEGAEFEMAVVDADGSARADLPGVASVRVFTTRPD
                     TSYGMTFCVLAPEHPLVEVITTAERRAEVDAFVERVRNTAEIDRLSAEGALADRGCAT
                     GAYALNPFTGTPVPIYLADYVLATYGTGAVMAVPGEDQRDWDFATAFGIPIVETVERP
                     DGWDGEAYSGPGLHVNSPVDPDAVPGPDLNGVEVAAAKDAAIAWLEEARHGERTVNFR
                     LRDWLVSRQRFWGCPIPIVYCEACGPRPVPLDELPVLAPDDVEFRPTGQSPLRFHEGF
                     LHTTCPDCGGPATRETDTMDTFVDSSWYFLRFCDPWSVDAPFSKEAVQDWMPVDQYIG
                     GAEHAVLHLMYARFFTKALSDLGVAPAALREPFTRLFTQGMIRLDGTKMSKSKGNLVA
                     PEEIIDTLGADTLRLAHLAVKPPEEDVDWEDFGLDGCARFLNRLWRLAVPGSDLLAAT
                     RDGDRSDADVAVVKATHRLIADVTDAFERWSYNVAVAKAMAFLNDLYRYVQSDAGAHG
                     QVVADAVDALLLLLAPAAPHITAELWSRRGAASGAPGRHVHEEPWPVPDADLLVEDAV
                     TMVVQVAGKVRDRIEVPADADEETCRALALASEKVRTQLGDAEPRKVIVRPPKLVNIV
                     P"
     gene            6721..6793
                     /locus_tag="PLZ72_13210"
     tRNA            6721..6793
                     /locus_tag="PLZ72_13210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6754..6756,aa:Ala,seq:ggc)
BASE COUNT         1049 a         2367 c         2521 g          897 t
ORIGIN      
        1 agcacggcgt cgtcggcggc agggtcctgg tgggtgcggt cgatggccag gttggccaca
       61 ccgtgtcggc gcgccgccca ggccaggaac cggtcacggc cccggaaggc gagcccgccc
      121 agcccgtcgg gcatggacag cagcacgacc agcacgccca gaccggtggc caggaacgac
      181 cacggtgccg gcagcagcca ctgggcgcca cgctggtaca cggccccgat gaccgccccg
      241 gccagcgagc ccagacctcc gatgacggtg gcgacgaaca cgtcgaggga ctgatccggt
      301 ccgtaggtga cgtcgcggaa cgccccctgg ctgatcacga gcaccacccc cgccaccgcg
      361 gccacggtgc cggagacggc gaaggcggtc agcttggccc ggatggtgga gatcccgtag
      421 acggtggcgc cggcctcgtt gtcgcgcagg gcgatcagga cccggccggt gcggctgttg
      481 cggatgccgc ccaacgccag gaccgacagg gccaggaccg cgagcgcgaa gtagtagatc
      541 cgcagcggtg tgtcgagcgc gatccggccg agcagagcgg gtcgggggaa cgacccgcgg
      601 gggatccagt ccgccacctt gttggagaag atccagtccg acgccgcgat cgacagtgcc
      661 agggtggtga ccgccaggta catgccccgc agccgcagtg ccgggagccc gaccgccacc
      721 gcgacggccg cgccgatgac gccggcgaga gccatcgcga cgagcgggtc ccagccctgt
      781 tcgaccatgg cccaggcgcc gacggccccg ccggcaccca cgaaggccat ctggccgagc
      841 gacacctggc cggcccagcc ggtgagcacc acgatcgatg tgccgatggt ggcgaacacg
      901 agcacgacac cggccttgat ctggccgttg gtgccgatca cctgcgggag cccgagggcc
      961 accagggcca ggagcagtcc tccggccagc cgaacggccc ggacctcggg caggcggacc
     1021 agcgaccacg gggtggggcg gacgtcgccg ctggtgcgcc acgacgaggt ctggtcctgc
     1081 tcggcccggc tggcaccccg ccgttcgagc agcaggctga ggacgatgag cccggccagg
     1141 aagggcgtga ccaaccagga ttcgcccgtg ttccagcgga tgcccgactc gaggatgccg
     1201 agggccaccg cggtggcggt gatggcgacc aggtgggtca tgcgtccgac caccagcgcc
     1261 gccagggccc gcaggagcac cgacaggccc aggccgatgc cgaacgggag gctggccacg
     1321 ccggcggtga gcaccaccga gaagaacgac agcaccgccg caccggtcca gacctgggcc
     1381 tcgagccgcc gcaccgggat gccgagcagg gcggcccgct cggcccgctc ggccgacgcc
     1441 cgcaccgcga caccggtgtc ggtgaacctc agcaccgacg cgaccaccgc cagcagcacc
     1501 ggcgcggcga cgagggcgat cacatcgttg gcgtcgaaga ccaccccgcc gagctcgacc
     1561 cgcagctcga acggcgtcgg cagcgagcgc accgcagggc cctcacccca caggtccggc
     1621 agggccagcg cggcgaaggc gagcacctgc gagaggccga tggtggcgac ggtcaggatg
     1681 aggcggggtg agcggtggaa ccgacggatg accagcacgt cggcgagcag cccggtcagc
     1741 accgcggcgc ccaacccggc agagatgccg agcagccacg gccagccggc cagggtgatg
     1801 agcagcaccg ccagcgtggc cgggaaggcg ccgaggtcgc cctgggcgaa gttcacgacc
     1861 cggttggccc gccagatcag cgccaggccc aacgcggaga gcgaccccaa caggcccagg
     1921 accactccgg acaggacgcc gccggcgggc atggggaacc agatgagctg gatggcgacg
     1981 accacggcgg ccggaccgat cgtccgggcg accgagcgca gcgcgtcctg gccggcgaag
     2041 gaccgcgccg gactcacccg cgcctccccc gtcgtgctgc cgaccgtcgc tgccgcaacc
     2101 gctgctgacc ccctggtcct gaccgaacgg tcaggttgac ccccgcccgt cggacccctc
     2161 ggccggcgtg gcgccagtca cactagaccg aacggtcaag tgcagggccg acccgacgcg
     2221 ggcgaccgac ggcggcagcg acggcggatc gaccgcggtg ggatcaggga cgctcgaggc
     2281 ggtcgagctc gtcggccagg tactggagcc gacacgccga gcgctgcaat ttgcccgacg
     2341 acgtcttggg caccgagccc ggctgcacga ggacgacctc cttgggcggc aggccgacgg
     2401 cgtcggtggc gacccgggtg atctcacgct cgagctcgtc gccggcagcc ttggcctcgg
     2461 cgaccacgat cacgttctgg gcgccctggc gaccctcgat cccgaaggcg atcacgttgc
     2521 cggtgcgcac cccctccacc tcgcccacga ccttctcgat gtcctgcggg tagatgttgc
     2581 gcccgccgac gatgatcacg tccttgatcc ggccgcagat caccagctcg ccgccgacgg
     2641 tgtaggccag gtcgccggtg ttgagccacc cgtcggtgat cagctccgcc gtcgcgtcgg
     2701 gctggttgta gtacccggtg gtcagcgagt tgccggcgat ccgcagctca ccgacccgtc
     2761 ggtcgccgca ctcctcaccg gtccgggggt cgcggataga gatctcgagg cccggcaccg
     2821 gacggcccag cagcgcgagc tcgaccgcgc cctcggtgtc ggagtcgacc gggaccgcct
     2881 tctggtcccg ctcgagcgcg gcacggtcga cgatgtcggt gatcaggccc ttgcccgggg
     2941 tggggaagca ccctgcgatg cacacctcgg ccatgccgaa ggccgggaac gccacgccgg
     3001 gctccagccc gaagcgctcc cccgcctcca ggaaccgccg gaacgtgggg gcgtcgatgg
     3061 gctcggcgcc gttgaggaac atccgcacct gcgacaggtc caggtcctcg tcggtgcgct
     3121 tgagagcgcg ggtggccaac gcgtacgaga agttcggacc ggccgacgcg gtgccgtgga
     3181 actggctcag ccaccgcatc cagcggatcg gcttggccag gaagtcctgg ggcgcgccct
     3241 gcaccaggtt ggtgtcgcgg gtcatcggga tggtcagcaa cccgaccaga cccatgtcgt
     3301 ggtagagggg cagccagctg acgatcacct cgtcgtggct gagctcggcg gagatccacg
     3361 ctccgtcgag gttgtggcag atgttctggt gcgggagcat cacccccttc ggctcggagg
     3421 tggagccgga ggtgaactgc aggatggcca gcgaggacgg gtcgtcggcg ggacgcacgt
     3481 agtcctcggc cgtggggccg tcggcgacga acagctcgtc gagcccgatg aagggcggat
     3541 ccccctcgac cggttcgacg aacgcggcca gctggctgtc gatgacgacg accttggagt
     3601 ccgaacgccg gatccgggtg cgggtctgct cgatgaactg gtcgagcgcc cccatccgca
     3661 tgggcagcgg catggtgacg agggcaccgc cggtcagcca gatcgcctgg atggcggtca
     3721 ccagccgacg cgtggtcgga cccagcaggg cgacgtgatc gccggggccg acaccacggg
     3781 cctgcagcgc ggcggccacg gcgcgggcct cggcgtgcag ctgaccccag gtgagggtgg
     3841 tgtggtcgtc gccgtcgacg aaggtcacct ttccgccgcc gtgctcggcg acggactcga
     3901 tgcgggagat cagggacagc tcgttcgaca gcacgtttcg ttcgactcct cggatgacga
     3961 gaaggttacg accgtcgctg gcgccggtcg tcacgacctc tcgcgggcgg tctcagggga
     4021 cggtctcagg ggacgatctc acgggacgat gttgaccagc ttcggcggtc ggacgatgac
     4081 cttgcggggc tcggcgtcac cgagctgggt ccggaccttc tccgacgcca gggcgagggc
     4141 ccgacacgtc tcctcgtccg cgtccgcggg cacctcgatg cggtcccgga ccttgcccgc
     4201 gacctgcacg accatggtca ccgcgtcctc gacgagcagg tcggcgtccg ggaccggcca
     4261 cggctcctcg tgcacgtggc ggccgggagc tcccgaggcg gcgccgcgcc gcgaccacag
     4321 ctcggcggtg atgtgcggag cggccggggc gagcagcagc agcagggcgt cgaccgcgtc
     4381 ggcgacgacc tgcccgtggg cgccggcgtc ggactgcacg tagcggtaca ggtcgttgag
     4441 gaacgccatc gccttggcga ccgccacgtt gtaggaccac cgctcgaagg catcggtcac
     4501 gtcggcgatc agccggtgcg tggccttgac gacggcgacg tccgcgtcgc tgcgatcgcc
     4561 gtcgcgggtg gcggcgagca ggtcggatcc gggcacggcc aggcgccaca gccggttgag
     4621 gaaccgggcg caaccgtcca ggccgaagtc ctcccagtcg acgtcctcct cgggcggctt
     4681 gaccgccagg tgcgccaggc gcagcgtgtc ggcacccagg gtgtcgatga tctcctcggg
     4741 tgcgaccagg ttccccttgg acttggacat cttggtgccg tcgaggcgga tcatgccctg
     4801 ggtgaacagc cgggtgaacg gctcgcgcag cgcggcgggg gcgacgccca ggtccgacag
     4861 cgccttggtg aagaagcggg cgtacatcag gtgcagcacg gcgtgctcgg ccccgccgat
     4921 gtactggtcg accggcatcc agtcctgcac cgcctccttg gagaacgggg cgtccacgct
     4981 ccacgggtcg cagaagcgca ggaagtacca cgacgagtcg acgaaggtgt ccatcgtgtc
     5041 ggtctcgcgg gtggcggggc cgccgcagtc ggggcacgtg gtgtgcagga acccctcgtg
     5101 gaagcgcagc ggcgactgtc cggtggggcg gaactcgacg tcgtcgggcg cgagcacggg
     5161 cagttcgtcg agcgggacgg gtcggggccc gcaggcctcg cagtacacga tcgggatcgg
     5221 acatccccag aaccgctggc ggctcaccag ccagtcccgg agccggaagt tgacggtgcg
     5281 ctcgccgtgc cgggcctctt cgagccaggc gatcgcggcg tccttggccg ccgcgacctc
     5341 gaccccgttg aggtccggac cggggacggc atcgggatcg accggcgagt tcacgtggag
     5401 gcccggcccg gagtacgcct cgccgtccca gccgtcggga cgttcgacgg tctcgacgat
     5461 cgggatgccg aacgcggtgg cgaagtccca gtcgcgctgg tcctcgcccg gcaccgccat
     5521 caccgcgccg gtgccgtagg tggccagcac gtagtcggcc aggtagatcg gcaccggggt
     5581 ccccgtgaag gggttgaggg cgtaggcacc ggtggcgcag ccccggtccg cgagggcgcc
     5641 ctcggccgag aggcggtcga tctcggcggt gttgcggacc cgctccacga acgcgtcgac
     5701 ctcggcgcgc cgctccgcgg tggtgatgac ctcgacgagc gggtgctcgg gggcgagcac
     5761 gcagaacgtc attccgtagc tggtgtcggg tcgggtggtg aacacccgga cgctggcgac
     5821 accgggaagg tcggcgcgcg ccgagccgtc ggcgtcgacg acggccatct cgaactcggc
     5881 gccctcggag cggccgatcc agttgcgctg ctgggtgacg accctttcgg gccactggac
     5941 cagctcgagg tcgtcgagga gctgctgggc gtagtcggtg atccgcaggt accactgctc
     6001 gaggtcgcgc cgctcgaccg cgtcgccgga acgctcgcag cgcccgtcga cgacctgctc
     6061 gttggcgagc accgtctggc atcccggaca ccagttgacc ggcgccgcct tgcggtaggc
     6121 gaggccggcc tcgaacagct tcaggaagat ccactgggac cagtggatgt actcggggct
     6181 gtggctgcgc acctcgcggc gccagtcgta gacacctccg atgcgggtga tcgagctgcg
     6241 cagcatctcg atgcgctcgt cggtgaaggt gcgcgggtgc acgccggtgt tgatcgccgc
     6301 gttctcggcg ggcagcccga agctgtcgaa gcccatcggc gacagcaccg cctcgccctg
     6361 catggtgcgg tagcgggtga ggaggtcgcc gaaggtgtag ttgcggacgt ggcccatgtg
     6421 cgccgcaccg gaggggtacg ggtacatgca cagcacgtag ctgcgcgggc ggggatcgtc
     6481 gttgtcgacc tcgtaggtgc cgcgctcacg ccagtaggcc tgccagtgcg gctcgatctc
     6541 ctggggtcgg taggggtcga tcgtcggcgc gtcgggggcg cccgaggggc cggcgctggc
     6601 agcttcggac atgggcggca gactaccgac ggcaccgagg ctgcccggaa ccgacgccga
     6661 accgtcgtgg tccggtcgat tcgcgacacc cgcgccggcc gactacagtg tcgccctcac
     6721 ggggccgtag ctcagttggg agagcgcttc catggcatgg aagaggtcgt gggttcaatt
     6781 cccatcggct ccactccggg gtctgtcggc gtcccgcacg ggacgtcgac atcc
//
LOCUS       DAOQOD010000166         6808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4286_length_6808_cov_9.527321, whole genome shotgun sequence.
ACCESSION   DAOQOD010000166 DAOQOD010000000
VERSION     DAOQOD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4286_length_6808_cov_9.527321"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1915)
                     /locus_tag="PLZ72_13215"
     CDS             complement(182..1915)
                     /locus_tag="PLZ72_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37303.1"
                     /translation="MAHDLVIRGGQLVDGTGAPARTADVTVDDGRISAVLEAGTAGVA
                     DREIDADGLMVTPGFVDIHTHLDAQVAWDPLGTSSSWHGVTSVVMGNCGVTFAPCKPE
                     DRTYLADMMESVEDIPAETIMAALPWDWQTYGDYLDSLERIDSVMNVGGMVGHCALRY
                     WAMGDRSMDPDARPSAAELDEMVGLLDEAMAAGALGFSTSRTLRHKVPDGRQVPGTFA
                     EPDELIALADVLARRGRGIFESAPRFDGDGPSEPRCRSELDWMREVALRSGRPVTFNL
                     THTFDNPDHHRLAIELAEAANAEGARIRPQTTARGIGVLFSLQSATPFGRHPSWAALR
                     ELDLPGRLAALRDPTTRARLIAEADTAPSAEELARFFVTGADGVPGQRDGIARYDCDP
                     RTALPAVAAERGVSMAEAYVDLCDESDGRVLVYWPLLNQSMDAIAEMLADDTVIMGLA
                     DAGAHVGQILDASQPTWFLTHWARDRGLLTPERAVQRLSSDTAAFVGLSDRGTVVPGM
                     AADLNVIDWEGLALPVPEFVHDLPKGAGRWIQRADGYVATVINGVVALENGEHTGAMP
                     GRVLRSGPDQR"
     gene            complement(2045..3163)
                     /locus_tag="PLZ72_13220"
     CDS             complement(2045..3163)
                     /locus_tag="PLZ72_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37304.1"
                     /translation="MTGPLEWRRSLAVGVLGWVWARVCVGAGFWVAHLVTPDPALAGR
                     QDFPLPKGLLSWDSFFYDSIARGGYHAVAADGARFFPLYPLLGRGLGVLFGGRSDVAL
                     VVIGNLAALAGAVVLAQLAREVLAPRWGSEESDAVGLRSAWMVAIVPAAIVFVWPYTE
                     GLALLATAGCLLALHRRAWWWVALCAAFAAALRPTGLLLGIPILIEVVATRPRPRIVP
                     ALAALVAAPLGLLASLAYVGNQVGDWMAPWEQQRPIRGDFRNPLVRAAESVWGLFHNT
                     DTELVPFVVLWCLLVVVSVRKRQPWSWVAFSGATLTVALAAQTIDSIGRYGLLAVPLV
                     VALAQWSDRRWRQVLVSCVGSAGLVVWTAQVLQGRIVP"
     gene            complement(3160..4878)
                     /locus_tag="PLZ72_13225"
     CDS             complement(3160..4878)
                     /locus_tag="PLZ72_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37305.1"
                     /translation="MGDHPEPSSRSGDSELIDPEAESAGTGTGTESPATIAPAPWWVR
                     VGIVVVVGAGIVARFLPRGSMWLDEALSANIAQLPLGDIGGALERDGHPPLYYVILHA
                     WGALVGWSPWALRALSGIFGVAALPLAFWAGRRIGGRPDADGHRDPIRARRTAVAALV
                     VLAALPFAIRYSSETRMYSLVMLLVLAGYLLVDHHRERPRWSTAIGTAIVAGLLLWSH
                     YWSLWLLGALGLVLLVQAVLDHRHGRTRRRNAELGLAAAIVVGALTFVVWLPTMLYQS
                     AHTGTPWGDVVRPAAFGVLGMIQVFGGNVAEPQLTAYLVAGVMVLGLFGLTNAAGRIE
                     LGWRVRPTARREGLIVLVTLAIAWAVSFVARAAFTGRYLAVVVPLVVLVIAMGLSAIT
                     RDGWRRAVAVVVVAGFAAGVAAEVGRVRTQAGEVADRIVEATAALPDGAPDPLVVTCP
                     DQNGPSVQRALRDVGLLDAEGQGRVRVVAVPRLDDPRFVDWVDYAERNRAIDPAEVAG
                     RITELGRDRTIFLAVTEGYKTLEGKCEAIQITLVSARGAPRILVTPDERNEDEPGMQL
                     LEFPAP"
     gene            complement(4871..6319)
                     /locus_tag="PLZ72_13230"
     CDS             complement(4871..6319)
                     /locus_tag="PLZ72_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNA37306.1"
                     /translation="MGTIDPARPHVEPSSPAAPSHPAVTAVIEITDTVALPYTTGLQR
                     VARELVSRLAADPDRSAVGAATDADADAAIRYRPTVWSVGADWYRDLTPDESDRLTHP
                     GSTMPASTALAERFPRPAATAIRRVLAVPAMRDLRSRARLAARRHAERPHLGLVLPPP
                     DRSTVLLDLEAAWNDPVPRDLLLGPWTRAGGASAALIADVLPLMRPEWFDSVLVRDFT
                     RFILGHLHHSDLFLCISERTRLDLLDVAAAHGIDRDLDTRVIPLGSDIVERSGAAENP
                     APAPTRATPQLPWAGAVPVERYLLMVGTVEPRKNQALALDVFDAVADAHPDLGLVLVG
                     KRGWKVEDLIERIERHPQAGRRILWLQEVDDDRLGELYRGAFLSLTPARYEGLGMTVT
                     EAMQFGTPVIASTAGALPEAGGDAAEYAGPDDVAAWVELIERHLTDVEHHRAAVARAR
                     AHRPPTWDDSADAVRVAMDDVFGARTQAALHG"
     gene            6426..>6808
                     /locus_tag="PLZ72_13235"
     CDS             6426..>6808
                     /locus_tag="PLZ72_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37307.1"
                     /translation="MSPGDDDARSVAGRGRLGGRSLAPVAGTFAAFGLFMGAWTVATP
                     EVERALGGGPARLGLVMSAALVVASGVNTVGGTLAERRGTTAALRGALVAWAVALAVG
                     AVMPAPWGVATGIVVVLSLGGVVDV"
BASE COUNT          967 a         2410 c         2607 g          824 t
ORIGIN      
        1 gagggcggca cggacgtcgt cgaggacgtc gaggacgggg aggcgcggtt cgttcgcggt
       61 cacgccgcgg gatgggacgc gggtgcggga cgggacgtga acgcgtcgcg ggacgtccgg
      121 gtgggccgga ttggcggccg gacggacggc cgggccgtgg ggtgacgcag cgtcggtccg
      181 gtcaccgctg gtcgggtccg gagcgcagga cccgaccggg catcgcgccg gtgtgctcgc
      241 cgttctccag cgccacgaca ccgttgatca cggtggcgac gtagccgtcg gcccgctgga
      301 tccaccggcc cgcgcccttg ggcaggtcgt ggacgaactc gggcaccggc agggcgagcc
      361 cctcccagtc gatcacgttc aggtcggcgg ccatgccggg aacgacggtg ccgcggtcgg
      421 acagtccgac gaaggccgcc gtgtccgacg agagccgctg caccgcacgc tccggtgtca
      481 gcaacccgcg gtcgcgggcc cagtgggtga ggaaccaggt gggctggctg gcgtcgagga
      541 tctgtccgac gtgggcgccg gcgtcggcca gacccatgat cacggtgtca tcggccagca
      601 tctcggcgat cgcgtccatc gactggttga gcagcggcca gtagacgagc acccgaccgt
      661 cgctctcgtc gcacaggtcg acgtacgcct cggccatcga cacgccccgc tcggcggcca
      721 ccgcgggcag cgcggttcgg gggtcgcagt cgtagcgggc gatcccgtct cgctggccgg
      781 ggaccccgtc ggccccggtg acgaagaagc gggccagctc ctcggcggac ggcgcggtgt
      841 cggcctcggc gatcaaccgg gcccgggtcg tggggtcacg caacgcggcc agccgaccgg
      901 ggaggtcgag ctcacggagc gcagcccacg acgggtgccg cccgaacggg gtggcggact
      961 gcaacgagaa cagcacgccg attccccgcg ccgtggtctg ggggcggatc cgggcaccct
     1021 cggcgttggc ggcctcggcc agctcgatcg ccaaccggtg gtggtcgggg ttgtcgaagg
     1081 tgtgggtcag gttgaacgtg accgggcgac cggaccgcag cgccacctcg cgcatccagt
     1141 cgagctccga tcggcagcgc ggctcggacg ggccgtcgcc gtcgaagcgg ggggcgctct
     1201 cgaagatgcc tcgcccgcgt cgggccagga cgtcggcgag ggcgatcagc tcgtcgggct
     1261 ccgcgaacgt gccggggacc tgacgtccat cggggacctt gtggcgcagg gtgcgcgagg
     1321 tcgagaaccc gagggcgccg gcggccatgg cctcgtcgag cagaccgacc atctcgtcga
     1381 gctcggcggc gctcggacgg gcatcgggat ccatggatcg gtcgcccatc gcccagtagc
     1441 ggagggcgca gtggccgacc atcccgccga cgttcatgac gctgtcgatc cgctcgaggc
     1501 tgtcgaggta gtcgccgtag gtctgccagt cccaggggag cgccgccatg atcgtctcgg
     1561 cggggatgtc ctcgacggac tccatcatgt cggccaggta ggtgcggtcc tcgggcttgc
     1621 agggagcgaa ggtcaccccg cagttgccca tgaccacgct ggtcacgccg tgccagctcg
     1681 agctggtgcc gagcgggtcc caggcgacct gggcgtcgag gtgggtgtgg atgtcgacga
     1741 agccgggcgt caccatcagg ccgtcggcgt cgatctcgcg gtcggcgact ccggcggttc
     1801 ctgcctcgag cacggcgctg atccggccgt cgtcgacggt cacgtccgcc gtgcgggccg
     1861 gagcgccggt cccgtcgacg agctgtcccc cacggatcac caggtcgtgc gccatggccg
     1921 cgaaccctac gcgccgacgg gtccttccgc gctcgccccg aggaccgctt tgtcccgcac
     1981 agacgcggtg atcaccccct ctgtgcggga caaagcagtc agggggccgc ggtcggggtg
     2041 gcggtcaggg gacgatgcgg ccctggagga cctgcgcggt ccagacgacg agcccggcgg
     2101 agccgacgca ggacaccagc acctgacgcc accggcggtc cgaccactgc gcgagggcca
     2161 ccaccaacgg cacggcgagc aggccgtagc gcccgatcga gtcgatggtc tgcgcggcca
     2221 gcgcgacggt cagcgtcgcc ccgctgaacg cgacccacga ccacggctgg cgcttccgca
     2281 ccgacacgac cacgagcagg caccacagca cgacgaacgg caccagctcg gtgtcggtgt
     2341 tgtggaacag accccagacc gactcggccg cccgcaccag cgggttgcgg aagtcgccgc
     2401 ggatcggtcg ctgctgctcc cacggcgcca tccagtcacc gacctggttg ccgacgtacg
     2461 cgagcgacgc gagaaggccg agcggcgcgg cgaccagggc ggccaacgcg ggcacgatcc
     2521 ggggcctggg ccgggtggcc accacctcga tcaggatcgg gatgccgagc agcaggccgg
     2581 tcggacgcag cgcagcggcg aacgccgcgc acagcgccac ccaccaccac gcccggcggt
     2641 gcagagcgag caggcacccg gcggtggcga gaagggcgag tccctcggtg tagggccaca
     2701 cgaacacgat cgcggcgggg acgatcgcca ccatccacgc tgatcgcaga cccacggcgt
     2761 cggactcctc ggacccccaa cgaggcgcga ggacctcgcg ggccagctgc gccagcacca
     2821 ccgcgccggc cagcgcggcc aggttgccga tgacgaccag agcgacgtcg ctgcggccgc
     2881 cgaacaggac cccgaggccg cgtccgagca acgggtacag cgggaagaag cgggcaccgt
     2941 cggcggcgac ggcgtggtag ccgccccggg cgatcgagtc gtagaagaac gagtcccacg
     3001 acagcaggcc cttgggcagc gggaagtcct ggcgtcctgc cagcgccggg tcgggggtca
     3061 ccaggtgggc gacccagaac cccgccccga cgcacacccg ggcccacacc cagccgagca
     3121 cgccgaccgc caggctgcgc cgccactcga gcggaccggt cacggcgcgg ggaactcgag
     3181 gagctgcatc cctggctcgt cctcgttgcg ctcgtcgggg gtgaccagga tccggggtgc
     3241 gccccgggcc gacacgagcg tgatctggat cgcctcgcac ttgccctcga gggtcttgta
     3301 cccctcggtc accgccagga agatcgtccg gtcccgcccc agctcggtga tccgcccggc
     3361 gacctcggcg gggtcgatcg cccggttgcg ctcggcgtag tcgacccagt caacgaagcg
     3421 ggggtcgtcg aggcggggga cggccacgac ccggacccga ccctggccct cggcgtcgag
     3481 caggccgacg tcgcgcagcg cccgctgcac cgacggcccg ttttggtccg gacaggtgac
     3541 gaccagcgga tccggcgcgc cgtcgggcaa cgcggccgtc gcctcgacga tgcggtcggc
     3601 gacctctccg gcctgggtgc ggacccgtcc cacctcggcg gccaccccgg cggcgaaccc
     3661 tgcgacgacg accaccgcca cggcccgtcg ccagccgtcg cgggtgatgg cggacagccc
     3721 catggcgatg acgaggacga cgagcgggac gacgaccgcc aggtaccgac ccgtgaacgc
     3781 agcgcgggcc acgaagctga ccgcccaggc gatggcgagg gtgacgagca cgatcaggcc
     3841 ctcccgacga gcggtgggcc ggacccgcca gccgagctcg atccgccccg cggcgttggt
     3901 gagcccgaac aggccgagca ccatcacacc ggcgaccagg taggcggtca gctgaggctc
     3961 ggcgacgttg ccgccgaaga cctggatcat cccgagcacc ccgaacgcgg cgggccgtac
     4021 gacgtcaccc cacggcgtgc cggtgtgggc gctctggtac agcatcgtcg gcagccagac
     4081 cacgaacgtc agcgcgccga ccacgatcgc tgcggccagg ccgagctcgg cgttgcgccg
     4141 ccgggtccga ccgtggcggt ggtcgagcac cgcctggacg agcagcacca ggcccagcgc
     4201 gccgagcagc cagaggctcc agtagtggct ccacaggagc agacccgcga cgatcgcggt
     4261 gccgatcgcg gtcgaccacc gggggcgttc gcgatggtgg tccaccagca ggtagccggc
     4321 caggaccagg agcatgacca gcgagtacat ccgggtctcg gaggagtacc ggatggcgaa
     4381 cgggagggcg gccaggacca ccagcgcggc caccgcggtg cgccgggccc ggatcgggtc
     4441 ccggtggccg tccgcgtcgg ggcgaccgcc gatgcgccgg ccggcccaga acgccagcgg
     4501 cagcgcggcg accccgaaga tgccggagag cgcccgcaac gcccacggcg accagccgac
     4561 cagagcgccc caggcgtgca ggatcacgta gtagagcggc gggtgtccgt cccgctccaa
     4621 cgcgccgccg atgtcaccga ggggaagctg ggcgatgttg gccgacaggg cttcgtcgag
     4681 ccacatcgac ccacggggca ggaaccgggc gacgatcccg gccccgacca ccacgacgat
     4741 gccgacgcgc acccaccagg gtgccggcgc gatcgtggca gggctctcgg tgccggtgcc
     4801 ggtgccggcc gactcggcct cgggatcgat cagctcagag tcacccgagc gggacgaggg
     4861 ttcggggtgg tcacccatgg agcgctgcct gcgtgcgggc tccgaacacg tcgtccatgg
     4921 cgacgcgcac cgcgtcggcg gagtcgtccc acgtgggcgg tcggtgggcg cgggcccggg
     4981 ccaccgcggc gcggtggtgt tcgacgtcgg tcaggtgccg ctcgatcagc tcgacccagg
     5041 ccgcgacgtc gtcgggtccg gcgtactcgg ccgcgtcgcc gccggcctcg ggcagggccc
     5101 cggcggtcga ggcgatcacc ggggtcccga actgcatggc ctcggtcacc gtcatgccca
     5161 gtccctcgta gcgggcgggg gtcagcgaca ggaacgcccc gcggtacagc tcgccgaggc
     5221 ggtcgtcgtc gacctcctgc aaccacagga tccgtcggcc ggcctggggg tgccgctcga
     5281 tccgttcgat caggtcctcg accttccagc cccgcttgcc gaccaggacc aggccgaggt
     5341 ccgggtgagc gtcggccacg gcgtcgaaca cgtcgagcgc cagggcctgg ttcttgcgcg
     5401 gctcgacggt gccgaccatc agcaggtacc gctcgacggg gaccgctccg gcccacggca
     5461 gctggggtgt cgcccgggtc ggggcgggtg ccgggttctc cgcggcgccg gagcgttcga
     5521 cgatgtcgga gccgagcggg atcacgcggg tgtcgaggtc gcggtcgatc ccgtgcgccg
     5581 cggccacatc gagcaggtcg agccgggtcc gttccgagat gcacaggaac aggtcgctgt
     5641 ggtggaggtg accgaggatg aaccgggtga agtcgcgcac gagcaccgag tcgaaccact
     5701 cgggtcgcat gaggggtagg acgtcggcga tcaacgccgc gctggcgcca ccggcccggg
     5761 tccacggccc gagcagcagg tcgcggggca ccgggtcgtt ccacgcggct tccaggtcga
     5821 gcagcacggt cgaccggtcg ggcgggggca ggaccaggcc gaggtgcgga cgctcggcgt
     5881 gacggcgggc cgcgagtcgg gcccggctcc gcaggtcgcg catggcgggg accgccagca
     5941 cccgtcggat cgccgtggct gcgggtcgcg ggaatcgctc cgccagcgcg gtgctggccg
     6001 gcatggtcga gcccgggtgg gtcagccgat cggactcgtc gggcgtgaga tcccggtacc
     6061 agtccgcccc gaccgaccac accgtcggcc ggtagcggat cgctgcgtcg gcgtcggcgt
     6121 cggtggcggc accgaccgcg gaccggtcgg ggtccgcggc gagccgtgac accagctcgc
     6181 gggcgactcg ctggagaccg gtcgtgtagg gcagcgccac ggtgtcggtg atctcgatca
     6241 ccgctgtgac cgccgggtgg gagggcgccg cgggggacga cggttcgacg tgggggcgcg
     6301 caggatcgat ggtgcccatg gtgggcggag cttacgggtc accgccccgg tgcactgcga
     6361 ccggttcccg tcgccggcgc acccgggcgg gccgtccgga ccccgggtcg tgggaagctc
     6421 cggcggtgag ccccggagac gacgacgccc gctccgtcgc gggtcgtggc cgcctcggcg
     6481 gccgatccct cgctcccgtc gccgggacgt tcgcggcgtt cggcctgttc atgggggcgt
     6541 ggaccgtggc gacgccggag gtggagcggg cgctcggcgg tggtccggcc cggctcggtc
     6601 tggtgatgag cgcggcgctg gtggtcgcct cgggggtcaa caccgtcggc ggcacgctgg
     6661 ccgagcgccg gggcaccacc gcggcgctcc gcggcgcgct ggtggcctgg gccgtcgccc
     6721 tggcggtcgg ggcggtgatg ccggcgccgt ggggggtggc caccggcatc gtggtcgtgc
     6781 tgtcgctcgg cggggtcgtc gacgtcat
//
LOCUS       DAOQOD010000167         6674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4403_length_6674_cov_10.613688, whole genome shotgun sequence.
ACCESSION   DAOQOD010000167 DAOQOD010000000
VERSION     DAOQOD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4403_length_6674_cov_10.613688"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2435)
                     /locus_tag="PLZ72_13240"
     CDS             complement(<1..>2435)
                     /locus_tag="PLZ72_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37308.1"
                     /translation="ILSTSTPTGGDMGAHVWGPAFLRDHLLPQFRLTGWTPDWYAGFP
                     AYVFYMVVPSLLIVMINVGPPLWLSPFLLAGWGWLAWVVARRVRNHVARTALWIALGV
                     AAVLSVPVPYEVAFKLVTVSGLVTLPLAAFALARSFRLPFPGPALVAVGAGAFLYETG
                     YTILGGNITSTMAGEFAFSISLTLCVLYLAVLVRGVRTGRRLALAAGLFALVILCHII
                     PALFAGAATVVLWLTRREDRIPWWDAFPVARGMAAGLVVVTSLTLVWRTDAFPVVATV
                     VVLLLFLGLDLRGVTFSALTLPVGGLLACFWFVPFFFDSAFMNDMGWEKYTKYAEYLW
                     PQPEQFDMPYRNVVFALAGLGVLLALVHRQRIGWFLAMVVVSMAWAFRFAPQWRLWNA
                     RILPFYYLALYLLAAVAVGLVIRSVSLVVADLARRREEPVLVGVIGAALVVTIVAVTI
                     AGALRILPGGHLDTDAAGVSSYRWMGLEWKRQNVAASWARYNYEGLEGRDAYPEFKAI
                     IDTMDRIGRDDGCGRAMWEYEPKLDRFGTPMALMLLPYFTDGCIGSMEGLYFEASSTT
                     PFHFLNQSELSSQPSRAQRDMPYSALDVTEGVSHLQMLGVKYYLATSDAAIAAARADD
                     RLTELTSITPAPTSDGVQHQWVVFEVAEADLVTPLRCEPVVLTDTDDHIDGWVYAKER
                     SAPAPGQEVGAKTAGPAVIWYQDPSRWDVPLATSGPSEWARVAPDDTDPACRPVPEVT
                     VSNVRDDGDSVSFEVSEPGTPILIRNSFFPNWQAEGADGPYRVSPNLMVVVPTDTHVT
                     VSYGTTNVDRLGWL"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2536..2845)
                     /locus_tag="PLZ72_13245"
     CDS             complement(<2536..2845)
                     /locus_tag="PLZ72_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37309.1"
                     /translation="MDPSPPPRSDAPPDPSDPDRPDVAEPVVPPDGTDGDQELIGTDG
                     DRELIGADDDRHSRHDLALIASWVVVALTCIFVFASLSPRGILSTSTPTGGDMGAHVW
                     G"
     gene            complement(2939..3136)
                     /locus_tag="PLZ72_13250"
     CDS             complement(2939..3136)
                     /locus_tag="PLZ72_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37310.1"
                     /translation="MSSCHTCRNELVEIGLTIDGSRLVMRSCSTCDTRSWHRDGESVE
                     LDGVLTDLSAVPTRYRRSLSS"
     gene            complement(3313..4035)
                     /locus_tag="PLZ72_13255"
     CDS             complement(3313..4035)
                     /locus_tag="PLZ72_13255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNA37311.1"
                     /translation="MVDTRSWMDPAQQRWGLGEVALGFLLAQALAVVATVVAMGAGGW
                     TEASDIPMAAAALLQVPLWGGYLGSVYLAGTKGGGLVDDFGLAVRPLDVPIGLVVGVA
                     VQLVVLPLLYVPILELTGTSQDDLSAPARALSDKATDPLGWVLLVLIVVIGAPLVEEL
                     FFRGLFLRALQKRGLPDWAAVVGSAAVFGAVHFQLLQFVGLFAIGLVLAVMAVRTGRL
                     GMSIATHMAFNATSVVVLYLTG"
     gene            complement(4049..4285)
                     /locus_tag="PLZ72_13260"
     CDS             complement(4049..4285)
                     /locus_tag="PLZ72_13260"
                     /inference="COORDINATES: protein motif:HMM:NF023430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HNA37312.1"
                     /translation="MSRSCARPGCGRPAVATLSYAYAEGVVWLEDLAAEDHPMVHDLC
                     LLHADTVRVPRGWELRDGRAVESLRGGRLDLIGA"
     gene            4410..6413
                     /gene="ftsH"
                     /locus_tag="PLZ72_13265"
     CDS             4410..6413
                     /gene="ftsH"
                     /locus_tag="PLZ72_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNA37313.1"
                     /translation="MPPEPNDAPTPPGKARPNGLPSWLLWVLVAVVGLTVVSALSLNS
                     AQQKQIAFSTFTEQVADGKVKTVTWNNVDQSITGTMKSGEDFVTTGPAEPTPELLQSM
                     EEHDVEVEFATPTPNLFVQLLPLLLPLLLIIGFFAWMNRRAQGQMSGIMSIGRSRAKT
                     YTTERPATLFTDVAGYEGVKTEIREVVDFLKEPERFLEIGAKIPKGVLLVGPPGTGKT
                     LIARAVAGEAGVPFLSVSGSDFMEMFVGVGASRVRDLFESARKHGRAIIFIDEIDSVG
                     RKRGAGLGGGHDEREQTLNQMLSEMDGFEGTDGVVMIAATNRPDILDPALLRPGRFDR
                     QVVVPLPELDDRKAILAVHVRQKKLSADVDLDLVARGTPGMSGADLANLVNEAALTAV
                     RRGSKVVEMHDFEDARDRVLMGQRRESMVLSDREKEITAYHEAGHALCAALLPNADPV
                     HKVTILPRGMALGVTQQLPEEEKHSYSQEFLEESIIVAMGGRVAEELVFDHRSTGAAN
                     DLQVSTERARKMVTEFGMSDEIGPRAWGTSGPVFLGDDFGHQREYSEDTARAIDAEVE
                     RLLRQGEDRCRDLLTRNRAALDLITRVLLEQETISGSEVNRLIRVANGTEPDLPAPTP
                     PVTPSPPVGGTPASVPPPVPPHLAPSPRPNVLPSQPFQGDVTA"
BASE COUNT         1086 a         2346 c         2330 g          812 t
ORIGIN      
        1 agccagccca ggcggtcgac gttcgtggtg ccgtagctga ccgtcacgtg ggtgtcggtg
       61 gggacgacca ccatcaggtt cgggctgacg cggtacggac cgtcggcccc ctcggcctgc
      121 cagttcggga agaacgagtt gcggatcagg atcggggtac ccggttcgga cacctcgaag
      181 ctcaccgagt cgccgtcgtc gcgcacgttc gacacggtca cctcgggaac cggccggcag
      241 gccgggtcgg tgtcgtcggg ggcgacgcgg gcccactccg acggtcccga cgtggccagc
      301 ggcacgtccc agcgagaggg gtcctggtac cagatcaccg cggggcccgc cgtcttggcg
      361 ccgacctcct ggccgggggc gggggcggag cgctcctttg cgtacaccca gccgtcgatg
      421 tggtcgtcgg tgtcggtgag cacgacgggt tcgcagcgca acggcgtgac caagtcggct
      481 tccgccacct cgaacacgac ccactggtgc tggacgccgt ccgaggtggg tgcgggggtg
      541 atcgaggtga gctcggtcag gcggtcatcg gcgcgggccg cggcgatcgc cgcgtcggag
      601 gtggccaggt agtacttcac gccgagcatc tgcaggtgcg acaccccttc ggtcacgtcg
      661 agcgccgagt agggcatgtc gcgttgggcc cggctgggct ggctggacag ctccgactgg
      721 ttgaggaagt ggaacggcgt cgtggagctg gcctcgaagt agaggccctc catcgagccg
      781 atgcagccgt cggtgaagta cggcagcagc atgagcgcca tcggcgtgcc gaaccggtcg
      841 agcttgggct cgtactccca catcgcccgg ccgcagccgt cgtcgcggcc gatgcggtcc
      901 atcgtgtcga tgatcgcctt gaactccggg taggcgtcgc gaccctcgag gccctcgtag
      961 ttgtagcggg cccaactggc cgcgacgttc tgacgcttcc actcgaggcc catccaccgg
     1021 tacgacgaca cgcccgccgc gtcggtgtcg aggtggcctc cgggcaggat gcgcagggcg
     1081 ccggcgatgg tgaccgcgac gatcgtgacc acgagcgcgg cgccgatcac cccgaccagc
     1141 accggctcct cgcgccggcg ggccaggtcg gccaccacga gcgagaccga gcggatgacc
     1201 aggcccaccg ccaccgcggc cagcaggtag agggccaggt agtagaaggg caggatccgg
     1261 gcgttccaca gccgccactg cggggcgaag cggaacgccc aggccatcga cacgacgacc
     1321 atggccagga accagccgat gcgctgacgg tggacgagtg cgagcaggac cccgagtccg
     1381 gccagcgcga acacgacgtt gcggtagggc atgtcgaact gctcgggctg cggccagagg
     1441 tactcggcgt acttggtgta cttctcccag cccatgtcgt tcatgaaggc gctgtcgaag
     1501 aagaacggca cgaaccagaa gcaggccagc agaccgccga ccggcagcgt gagggcggag
     1561 aacgtgacac cgcgcaggtc gaggccgagg aacagcagca gcaccacgac cgtggcgacc
     1621 accgggaagg cgtcggtgcg ccagacgagc gtcagcgacg tcacgaccac caggccggcc
     1681 gccatgcccc gcgccacggg gaacgcgtcc caccacggga tgcggtcctc gcgccgggtc
     1741 agccacagca cgacggtggc ggctccggcg aacagcgccg ggatgatgtg gcacaggatg
     1801 acgagggcga acaggcccgc cgcgagcgcc agccgacgac ccgtgcgcac accccggacc
     1861 agcaccgcca ggtacaggac gcacaacgtg agcgagatgg agaaggcgaa ctcgcccgcc
     1921 atcgtcgagg tgatgttgcc gccgaggatc gtgtagccgg tctcgtagag gaacgcaccc
     1981 gcgcccaccg ccaccagagc gggaccgggg aagggcagac ggaacgagcg ggcgagcgcg
     2041 aacgcggcca gcggcagggt gaccagcccc gacacggtca ccagcttgaa ggcgacctcg
     2101 tagggcaccg ggacgctcag gaccgcagcc acccccaggg cgatccacag ggcggtccgg
     2161 gccacgtggt tgcggacccg gcgggcgacg acccacgcca accagcccca ccccgccagc
     2221 aggaacgggc tgagccacag cggaggcccg acgttgatca tcacgatcaa cagcgaaggg
     2281 accaccatgt agaagacgta ggcggggaac cccgcgtacc agtcgggcgt ccagccggtg
     2341 agccggaact gcggcagcag gtggtcgcgc aggaacgccg gaccccacac gtgggcgccc
     2401 atgtcgccgc cggtcggcgt ggaggtgctg aggatnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnngaccc cacacgtggg cgcccatgtc gccgccggtc ggcgtggagg
     2581 tgctgaggat tccccgcggg ctgagcgagg cgaagacgaa gatgcaggtc agcgcgacga
     2641 cgacccagga ggcgatgagc gcgaggtcgt ggcgcgagtg gcggtcgtca tcggcgccga
     2701 tcagttcccg gtcgccgtcg gtgccgatca gctcctgatc gccgtcggtg ccgtccgggg
     2761 ggacgacggg ctccgccacg tcgggccggt cggggtcgct cgggtccggg ggcgcgtcgg
     2821 agcgcggtgg ggggctcgga tccatggggg cctcatcgtg tcagatcccg cccatcgcat
     2881 cgggtcaccg ccgtgcgaca ccacgagccg gaacgggacc caccgacccc gcagagggtc
     2941 aggacgacag cgaccgacgg taccgggtcg ggaccgcgct caggtcggtc agcaccccgt
     3001 cgagctcgac cgactcgccg tcgcggtgcc agctgcgggt gtcgcaggtc gaacaggagc
     3061 gcatgaccag ccgtgagccg tcgatggtca gcccgatctc gacgagctcg ttgcggcacg
     3121 tgtgacagct cgacattggt tcctcccagc tcgcggaacg accccagcgg gccgcggtcg
     3181 ccgcggacgc ggtcctcggc gacctctctc cgtcggcagg acggcgcggc gccgtgagcg
     3241 atccggccgg gaatgatccg ttcggcccag tcgacagcgg gtgtcgtgtc gaccgcgcag
     3301 cggcgcggcg ggtcagccgg tcaggtagag cacgaccacg ctggtggcgt tgaaggccat
     3361 gtgggtggcg atcgacatgc ccagtcgccc ggtccgcacc gccatcaccg ccaggacgag
     3421 cccgatggcg aacaggccga cgaactgcag cagctggaag tgcaccgccc cgaacaccgc
     3481 ggcggacccg accaccgccg cccagtcggg cagtccccgc ttctgcagcg cacgcaggaa
     3541 caggccccgg aagaacagct cctcgaccag cggcgcgccg atcaccacga tcaggacgag
     3601 cagcacccaa ccgagcgggt cggtcgcctt gtccgacagg gcccgagcgg gagcggagag
     3661 gtcgtcctgg ctcgtgccgg tcagctcgag gatcggcacg tacagcagag gcagcaccac
     3721 cagctggacc gcgacgccga cgaccaggcc gatcgggacg tcgagcggtc ggaccgccag
     3781 gccgaagtcg tcgacgagtc cgccgccctt ggtcccggcc aggtacaccg accccaggta
     3841 tccgccccac aacggcacct gcagcagcgc ggcggccgcc atcgggatgt cggaggcctc
     3901 ggtccaaccg ccggcgccca tcgccacgac ggtggcgacc acggccaggg cctgggccag
     3961 gaggaagccg agcgcgacct cgccgagacc ccaccgctgc tgcgccgggt ccatccagga
     4021 ccgggtgtcg accacgacgg ggggacgctc aggcgccgat gaggtcgagc ctgccgcccc
     4081 gcagggactc cacggcccga ccgtcgcgca gctcccaacc ccggggaacc cgcaccgtgt
     4141 cggcgtgcag caggcagagg tcgtgcacca tcgggtggtc ctcggcggcg aggtcctcga
     4201 gccacaccac gccctcggcg taggcgtacg acagcgtggc gaccgccggg cggccgcatc
     4261 cgggacgggc gcagctcctg ctcacggcgg gcaccgtacc ggcgggtcgt cgtcggggtg
     4321 gggatgcccc gacgccgacg acacgggccg atggcaccct tctcaggctc ggcatgtcgg
     4381 aacgcgggtc gggctcctag gctgagggga tgcctcccga gcccaacgac gcccccacgc
     4441 cccccggcaa ggcccgaccc aacggcctgc cgtcgtggct gctgtgggtg ctggtcgcgg
     4501 tggtcggact caccgtcgtg agcgccctgt cgctgaactc cgcccagcag aagcagatcg
     4561 ccttctccac cttcaccgag caggtcgccg acggcaaggt caagaccgtc acctggaaca
     4621 acgtcgacca gtcgatcacg ggcaccatga agtccggcga ggacttcgtc accaccggcc
     4681 ccgccgagcc cacccccgag ctgctgcagt ccatggagga gcacgacgtc gaggtcgagt
     4741 tcgccacccc gacgcccaac ctgttcgtcc agctcctacc gctgctgctg ccgctgctgc
     4801 tgatcatcgg cttcttcgcc tggatgaacc ggcgagcgca gggccagatg agcgggatca
     4861 tgtcgatcgg acgctcccgg gccaagacct acacgaccga gcgcccggcc acgctgttca
     4921 ccgacgtcgc cggctacgaa ggcgtcaaga ccgagatccg cgaggtcgtc gacttcctca
     4981 aggagccgga gcgcttcctc gagatcggag ccaagatccc caagggcgtg ctgctggtcg
     5041 gccctccggg caccggcaag accctcatcg cccgggccgt cgccggcgag gccggcgtcc
     5101 cgttcctgtc ggtgtcgggg tcggacttca tggagatgtt cgtcggcgtc ggcgccagcc
     5161 gggtccgcga cctgttcgag tcggcccgca agcacggccg ggcgatcatc ttcatcgacg
     5221 agatcgactc ggtcggccgc aagcgcggcg ccggcctcgg cggcggacac gacgagcggg
     5281 agcagacgct caaccagatg ctctcggaga tggacggctt cgagggcacc gacggcgtcg
     5341 tcatgatcgc ggccaccaac cggcccgaca tcctcgaccc ggcgctgctg cgcccgggtc
     5401 gcttcgaccg ccaggtcgtc gtcccgctgc ccgagctgga cgaccgcaag gcgatcctgg
     5461 ccgtgcacgt ccgccagaag aagctgtcgg ccgacgtcga cctggacctg gtcgcccgcg
     5521 gcacgccggg catgtcgggc gcggacctgg ccaacctggt caacgaggcc gccctcaccg
     5581 cggtgcgacg cgggtccaag gtcgtcgaga tgcacgactt cgaggacgcc cgggaccgcg
     5641 tcctcatggg ccagcggcgc gagtcgatgg tgctgtcgga ccgggagaag gagatcaccg
     5701 cctaccacga ggcgggccac gccctgtgcg cggcgctgct gcccaacgcg gatccggtcc
     5761 acaaggtgac gatcctgccc cggggcatgg cgttgggcgt cacccagcag ctccccgaag
     5821 aggagaagca ctcctacagc caggagttcc tcgaggagtc gatcatcgtg gcgatgggcg
     5881 gccgcgtcgc cgaggagctc gtgttcgacc accgctccac cggtgccgcc aacgacctcc
     5941 aggtctccac cgagcgggcc cggaagatgg tcaccgagtt cggcatgagc gacgagatcg
     6001 gaccccgagc gtggggcacc tccgggccgg tcttcctggg cgacgacttc ggccaccagc
     6061 gcgagtactc cgaggacacc gcccgggcga tcgacgccga ggtcgagcgg ctgctgcgac
     6121 agggcgagga ccgctgtcgc gacctgctca cccgcaaccg cgccgcgctg gacctgatca
     6181 ccagggtgct gctcgaacag gagacgatct cgggcagcga ggtgaaccgg ttgatccgcg
     6241 tcgccaacgg caccgaaccc gacctgccgg cgcccacgcc gcccgtcaca ccgtcgccgc
     6301 cggtgggcgg aacccccgcg tcggtacccc ctccggtgcc cccgcacctc gccccctccc
     6361 cccggccgaa cgtgctgccc agccagccgt tccagggaga cgtcaccgcc tgacccgagt
     6421 ccggccgagc cacagtcgac ccggctgcac ctcggtcgct tccaacgcac ctcggggcgc
     6481 gtcggccccg atcgcgccgg gggcagatgg gcctttcgac ccaccctggg tcattcgcct
     6541 caagtccgat gggccgatcg gccgaagtgc gccccatgca cgacgacggc ggaacactga
     6601 cggtggagcg ggccccgacc cgacgatcgg tcctcggggc ggccggagcg ggcgggatcg
     6661 ccctggccgc ggca
//
LOCUS       DAOQOD010000168         6671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4408_length_6671_cov_9.744256, whole genome shotgun sequence.
ACCESSION   DAOQOD010000168 DAOQOD010000000
VERSION     DAOQOD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4408_length_6671_cov_9.744256"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLZ72_13270"
     CDS             complement(<1..532)
                     /locus_tag="PLZ72_13270"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNA37314.1"
                     /translation="MEAPHPRRVTRRSGSQVLPLELFFDLVFVLAITQCTALMAAQPT
                     WAGLGRGLLVLAVLWWAWTAYAWLTSVIDPEADATRITMFAAMSAMLVASLCVPQAFG
                     DLALAFAMAYAAVRVIQIVLFVLASPDDGDLRRSVTSLAASTTVAVALLVAASFLDGI
                     AQGAMWVLAILFDVGGP"
     gene            complement(539..2284)
                     /locus_tag="PLZ72_13275"
     CDS             complement(539..2284)
                     /locus_tag="PLZ72_13275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNA37315.1"
                     /translation="MLPAAPTPRPFVRSVALEALRTESFDLTVIGGGITGAGVALDAA
                     ARGMRVALIDAGDFSSGTSSKSSKLIHGGLRYLQQGEIGLVYQALAERQRLLRNAPHL
                     VKLLPFLIPMFGSGGVIPAKISRLLGSAMWGYDLTGGWRVGKRHERLDFDQALALMPT
                     LPPDRLVSAYLYYDAAADDSRLVVTVLRTAVLEFGAACANHVRAVGLRKDGQGVVDGV
                     DVEVLDPGSGTGGPERFTISTRAVVNASGVWSDQVRTLDEGADPDSIRPAKGIHITVP
                     WSKVRNQVAAVVPVPGDKRSVFVIPNGDLTYIGTTDTDYDGPVDDPQCSAEDVAYLLS
                     AINAASTEQLGPSDVVGSWAGLRPLVKAPDADGPAEGRTADLSRRHRVAVSPSGVITI
                     TGGKLTTYREMAADTVDEAIESVLARRPGFAGYGSSPTRDLALRGAAGHDTVHDAAEI
                     YPRVDREHLDHLAGRYGGEARVIMASIEADPTLGEPLVVGLPYLRAEAIFAVRHEMAR
                     SVDDVLSRRTRARLLGRDDSAAAADDVAALIAEELGWDEPTAAASAEAYRRSVAHERE
                     SADLPETHLEQLLGA"
     gene            2437..2730
                     /locus_tag="PLZ72_13280"
     CDS             2437..2730
                     /locus_tag="PLZ72_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA37316.1"
                     /translation="MDRGWLNESACRGLDPAIFYPLTDEDAEEAKSICGVCPVRDACL
                     EHAIGHREHNGVWGGATERERQRIIRRRRRLRALAASGSATVGAGEAAGAVGH"
     gene            2751..3812
                     /gene="trpD"
                     /locus_tag="PLZ72_13285"
     CDS             2751..3812
                     /gene="trpD"
                     /locus_tag="PLZ72_13285"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNA37317.1"
                     /translation="MGAFAAIGAWPGVLARLVAGEDLPADVASAALDRILAGEATDAQ
                     IAGFLIGLRAKGEAPDEVAGLVEAMLAAASPLELDDPAATVDIVGTGGSVALGGQAFN
                     VSTMASIVAAAAGATVCKHGNRKASSTSGSTDLLEALGVEVDLDGPGVQACVREAGVG
                     FAFARMFHPAMRHVGPVRTELGVPTVFNLLGPLSHPGRVRRQVLGVTNAAHVDLVAGV
                     LARRGVLRAWVVRGSDGLDELTTTDATDVIELIDGVEVRRFRVTPEELGLRRATTTDI
                     AVGDPEANAAAATSVLQGRSGPVRDMVVLNAAAALVVADVATDLADGLERAARAVDSG
                     EAARTLGELVAVSRAVRGD"
     gene            complement(3787..5541)
                     /locus_tag="PLZ72_13290"
     CDS             complement(3787..5541)
                     /locus_tag="PLZ72_13290"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HNA37318.1"
                     /translation="MAPAAGSTPTERPDRYRQTSLDDLGTPLVDTTFVVVDLETTGGS
                     PGSDTITEVGAVKVRGGEVLGTFQTLVNPGRAIPPTITVLTGITESMVVRAPRIETVL
                     PSLLEFMGDSVVVGHNVRFDVGFLQAALERCGRAPLTAPTVDTVALARRLVRDEVPDC
                     RLGTLAERFRLSHRPSHRALDDALATADLLHLLLERAGRMGVAGLDDLLGLPSMAGHP
                     QADKLRLTERLPRRPGVYLFRAADGRVLYVGKATNLRSRVRSYFSTDERRKIGSLLRE
                     TARIDHKVCPSTLEASVLEARLIRALEPRFNSHGTRWRRYPYLKLTLDEPFPRLSVVR
                     SVRDDGGLYLGPLRSARQAREVADAIESVVPLRRCNGTVRTRRSDPDDDPTSVASRSA
                     PCASAQLGVSSCPCAGGVARSEYARHVQRVVDGLTGRSDLLVEPLRQRMADVAAAERF
                     EEAAAARDRLAALVDALSRQRRLDQLRGCSRLWVQVESGEVAEVRHGVLWQMWPAEWA
                     DQLAPVARPVEMRPGAPPDPQIPVDRGMADEVACVASWLEQHAGRIRLERSEGTLASV
                     LPRLPDVRPRISPRARRG"
     gene            5616..5930
                     /locus_tag="PLZ72_13295"
     CDS             5616..5930
                     /locus_tag="PLZ72_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37319.1"
                     /translation="MTSPSEPPVDTTAPHVADAAAVRYRCTSCGNLTRFDVTTTRRTR
                     AFHHYTVGGELEVEDLSVLAEELESVECRWCGPSGVVVEIDADAAAALDRSGSGASDP
                     EG"
     gene            6129..>6671
                     /locus_tag="PLZ72_13300"
     CDS             6129..>6671
                     /locus_tag="PLZ72_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37320.1"
                     /translation="MAASASEDDVGRAGWIFLDRPEGWNDELDLLVAVEQERSERDRS
                     EAEERSARHRAEHLERSVEGLRSRLGAAVAEADAARRALDAERSRRRELEREVEAHRR
                     RTEAAVAERDRATAELAEARRIADDRLSRERSARELLSRFEAERDRWVAPVDQALVDA
                     RAVLDELAAALDVAGAALRRG"
BASE COUNT          906 a         2351 c         2567 g          847 t
ORIGIN      
        1 acggtccgcc gacgtcgaac aggatcgcca gcacccacat ggccccctgc gcgatgccgt
       61 cgaggaacga cgccgcgacg agcagggcga cggcgaccgt cgtgctggcg gccaggctgg
      121 tcaccgacct gcgcaggtcg ccgtcgtcgg ggctggccag cacgaacagc acgatctgga
      181 tcacccgtac cgccgcgtag gccatggcga aggcgagcgc gaggtccccg aacgcctgag
      241 gcacgcacaa cgacgcgacg agcatggcgc tcatggccgc gaacatggtg atccgggtcg
      301 cgtcggcctc ggggtcgatg accgacgtca gccacgcgta cgcggtccac gcccaccaca
      361 gcacggccag gaccaggagc ccgcggccga gcccggccca cgtcggctgg gccgccatca
      421 gcgccgtgca ctgggtgatg gccagcacga agaccaggtc gaagaacagt tcgagcggca
      481 ggacctgtga tcccgaacgc ctggtgaccc ggcgcgggtg gggagcctcc acggcgtctc
      541 aggcgccgag cagctgctcg aggtgggtct cgggcaggtc ggccgactcg cgctcgtggg
      601 cgacggagcg acggtacgcc tcggcggacg cggcggcggt cggctcgtcc cagccgagct
      661 cctccgcgat gagggcggcc acgtcgtcgg cagcggccgc cgagtcgtcg cggcccagga
      721 gtcgggcgcg ggtccgccgc gacaggacgt cgtcgacgct gcgggccatc tcgtggcgga
      781 ccgcgaagat cgcctcggcg cggaggtacg gcaggccgac caccaggggt tcgcccaggg
      841 tcgggtcggc ctcgatcgac gccatgatca cccgggcctc tccgccgtag cggccggcca
      901 ggtggtcgag gtgctcccga tcgacccggg ggtagatctc ggccgcgtcg tgcacggtgt
      961 cgtggccggc cgcgccccgc agggccaggt cccgggtggg gctcgagccg tagccggcga
     1021 agccgggtcg acgggccagg accgactcga tcgcctcgtc gacggtgtcg gcggccatct
     1081 cgcggtaggt ggtcagcttc ccgccggtga tcgtgatcac ccccgacggc gacaccgcga
     1141 cccggtggcg tcgtgagagg tccgcggtgc gaccctcggc gggtccgtcg gcgtcgggtg
     1201 ccttgaccag cgggcggaga ccggcccagg agccgacgac gtcggacggc ccgagctgct
     1261 cggtgctggc cgcgttgatg gccgacagca ggtaggcgac gtcctcggcg ctgcactggg
     1321 ggtcgtcgac cgggccgtcg tagtcggtgt cggtggtgcc gatgtaggtg aggtcgccgt
     1381 tggggatcac gaacaccgag cgcttgtcgc cggggacggg gacgaccgcg gcgacctggt
     1441 tgcggacctt ggaccacgga accgtgatgt gtatgccctt ggcgggccgg atcgagtcgg
     1501 ggtcggcgcc ctcgtcgagg gtgcggacct ggtcggacca cacacccgag gcgttgacga
     1561 ccgcacgggt cgagatcgtg aaccgctccg ggcccccggt cccggacccg gggtcgagca
     1621 cctcgacgtc gacaccgtcg acgacgccct gtccgtcctt gcgcaggccg acggcgcgca
     1681 cgtggttggc gcacgcggcg ccgaactcga gcacggcggt gcgcaggacc gtgacgacca
     1741 gacgggaatc gtccgccgcg gcgtcgtagt acaggtacgc ggacaccagc cggtccggcg
     1801 gaagcgtggg catgagagcc agggcctggt cgaagtcgag gcgttcgtgg cgcttgccga
     1861 cccgccaccc gccggtcagg tcgtagcccc acatggccga gcccagcagg cgtgagatct
     1921 tggccgggat caccccgccc gaaccgaaca tggggatgag gaacggcagc agcttgacca
     1981 ggtgcggtgc gttgcgaagc aggcgctgac gttcggccag ggcctggtac accagcccga
     2041 tctcgccctg ctggaggtac cgcaggccgc cgtggatgag cttggacgac ttcgacgacg
     2101 tgcccgacga gaagtcgccg gcgtcgatca gggcgacgcg catgccgcgg gcggccgcgt
     2161 cgagggcgac cccggccccg gtgatgccgc cgccgatgac ggtgaggtcg aacgactcgg
     2221 tccgcagcgc ctcgagggcg acggaacgca cgaagggacg gggtgtgggt gcagccggga
     2281 gcaccccgac aggttacgac cccgtccggt gacgtcccga ctccgacctg ctcagccacg
     2341 tccggccttc ggggatcccg ggtccacggc cccggcggag tccaccgtcg gcctcctttg
     2401 gacacccgcg acacgcggtg ggcagaatcg ccccacatgg accgcggttg gctgaacgag
     2461 tccgcatgtc gggggctcga tcctgcgatc ttctatccgc tcaccgacga ggacgccgag
     2521 gaggctaagt cgatctgtgg cgtctgcccg gtccgcgacg catgcctgga gcacgcgatc
     2581 ggccaccgcg agcacaacgg cgtgtggggc ggcgccaccg agcgggaacg ccagcgcatc
     2641 atccggcgtc ggcgccggct gcgggcgctc gccgcgtcgg gatccgccac ggtcggcgcc
     2701 ggcgaggccg ccggcgccgt gggccactga gtcgggtcgg ggagaggcac gtgggggcgt
     2761 tcgcggcgat cggggcgtgg cccggggtcc tggcccggct cgtcgccggt gaggacctgc
     2821 ccgcggatgt cgcctccgca gcgttggacc ggatcctggc cggcgaggcc accgacgccc
     2881 agatcgcggg gttcctgatc gggctgcggg ccaagggaga ggcgcccgac gaggtcgcgg
     2941 ggctcgtcga ggccatgttg gccgcggcgt cgccgctcga gctcgacgac ccggccgcga
     3001 cggtcgacat cgtcggcacc ggcgggtccg tggcgttggg cggccaggcg ttcaacgtgt
     3061 cgacaatggc ctcgatcgtc gccgccgccg ccggagcgac ggtgtgcaag cacggcaacc
     3121 gcaaggccag ctccacctcg gggtccaccg acctgctcga agccctcggc gtcgaggtcg
     3181 acctcgacgg gcccggcgtg caggcctgcg tgcgggaggc gggcgtcggc ttcgccttcg
     3241 cccggatgtt ccatccggcc atgcgccacg tcggtccggt ccgcaccgag ctcggcgtcc
     3301 ccacggtgtt caacctcctc ggcccgctgt cgcacccggg acgggtgcgc cgccaggtcc
     3361 tcggcgtcac caacgccgcc cacgtcgacc tggtcgccgg agtgctggct cggcgcgggg
     3421 tgctccgggc ctgggtggtg cgcggatccg atggcctgga cgagctgacg acgaccgacg
     3481 ccaccgacgt catcgagctg atcgacggcg tcgaggtccg gcggttccgg gtgacgcccg
     3541 aggagctcgg actgcgccgg gcgaccacga ccgacatcgc ggtcggggac cccgaggcca
     3601 acgccgcggc ggccacctcg gtgctgcagg gtcgatccgg acccgtgcgc gacatggtcg
     3661 tgctgaacgc ggccgccgcc ctggtggtgg ccgacgtcgc gaccgatctc gccgacggac
     3721 tcgagcgggc cgcccgcgcc gtcgactcgg gcgaggccgc ccgcaccctc ggggagctgg
     3781 tggcggtcag ccgcgccgtg cgcggggact gatccggggc cggacgtcgg ggagtcgggg
     3841 cagcaccgac gccaacgtgc cctccgatcg ctccaggcgg atccggcccg cgtgctgctc
     3901 caaccacgag gccacgcacg cgacctcgtc ggccatgccg cggtccaccg ggatctgcgg
     3961 gtcgggtggg gcgccgggtc gcatctcgac gggccgggcg accggggcga gctggtcggc
     4021 ccactccgcc ggccacatct gccacaggac gccgtggcgc acctcggcca cctcaccgga
     4081 ttcgacctgc acccacagcc tggagcagcc gcggagctgg tccagccgtc gttggcgtga
     4141 cagggcgtcg acgagggcgg ccaggcgatc gcgggctgcg gcggcctcct cgaatcgctc
     4201 cgccgcggcc acgtcggcca tgcgctgccg gagcggctcg accagcagat cgctgcggcc
     4261 ggtgagcccg tcgacgaccc gctggacgtg gcgggcgtac tcggagcggg ccacaccgcc
     4321 cgcgcacgga cagctcgaca cgccgagctg ggccgaggcg cacggcgccg accgggacgc
     4381 caccgaggtg gggtcgtcgt cggggtcgct gcgacgggtc cggaccgtgc cgttgcagcg
     4441 gcgcaacggc acgaccgact cgatggcgtc ggccacctcg cgggcctggc gggcggagcg
     4501 cagcggaccc aggtacaggc cgccgtcgtc gcgaaccgag cggaccaccg acaggcgagg
     4561 gaacggttcg tcgagggtca gcttgaggta ggggtagcga cgccagcggg tgccgtggga
     4621 gttgaaccgg ggttcgaggg cccggatgag gcgggcctcg agcaccgacg cctccagggt
     4681 cgacgggcac accttgtggt cgatgcgggc ggtctcgcgc agcagcgatc cgatcttgcg
     4741 gcgctcgtcg gtggagaagt agctgcgcac ccggctgcgc aggttggtgg ccttgcccac
     4801 gtagagcacc cgcccgtcgg cggcccggaa caggtacacg cccgggcggc gggggagccg
     4861 ctcggtcagg cgcagcttgt cggcctgggg gtgaccggcc atggagggca ggccgagcag
     4921 gtcgtcgagt ccggccacgc ccatgcgacc ggcccgctcg aggagcaggt gcagcagatc
     4981 cgcggtggcg agggcgtcgt cgagggcgcg gtggctgggc cggtgcgaca gccggaagcg
     5041 ctcggcgagc gtccccaggc ggcagtcggg aacctcgtcg cgcaccagac gccgggcgag
     5101 ggcgacggtg tcgaccgtgg gagcggtcag cggtgctcgg ccgcaccgct cgagcgcggc
     5161 ctggaggaac ccgacgtcga agcggacgtt gtggccgacc accaccgagt cgcccatgaa
     5221 ctcgagcagg ctgggaagca ccgtctcgat gcggggcgcc cgcaccacca tcgactcggt
     5281 gatgccggtg agcacggtga tggtgggtgg gatcgcccgc cccgggttga cgagggtctg
     5341 gaaggttccg agcacctcgc cgccgcggac cttgaccgcg ccgacctcgg tgatggtgtc
     5401 ggagccgggc gaccctccgg tggtctccag gtcgaccacg acgaacgtgg tgtccaccag
     5461 cggcgtgccg aggtcgtcca gactggtctg gcggtagcgg tcgggccgtt cggtcggcgt
     5521 gctgcccgcg gcgggtgcca tcgacccagt ctgctcgaac gtgtgttcga tcgtcaacgg
     5581 tcggccgggt gacccggacg ataggatcgg cgccgatgac ctccccctcc gagcccccgg
     5641 tcgacaccac cgccccacac gtcgccgacg ctgccgcggt ccggtaccgc tgcacgtcgt
     5701 gcggcaacct gacccgcttc gacgtgacca ccacccgacg cacccgggcc ttccaccact
     5761 acacggtggg cggggagctc gaggtcgagg acctcagcgt gctcgccgag gagctcgagt
     5821 ccgtcgagtg ccgctggtgc ggaccgtcgg gcgtggtggt ggagatcgac gccgatgctg
     5881 cggccgcgct cgaccggtcg gggtcgggcg cgtcggaccc cgagggctga gcggtcgacg
     5941 ccgctccgct gctcacggat gtcgccgagc tggcgctcgc ggtcgcccga cgggacctcg
     6001 gcgccaacga cgcgccccca cctcccccct cgatccgccc tctgctgcgg ttccgcggcc
     6061 gtctgagcac ccgggcggtc gacacgctgc tgcaggcgct cgacggcaac gacgagttcc
     6121 gctcccgggt ggctgcatcc gcgagcgagg acgacgtcgg tcgggccggg tggatcttcc
     6181 tcgaccgacc cgagggttgg aacgacgagc tcgacctgct cgtcgccgtc gagcaggagc
     6241 gatccgagcg cgaccgcagc gaggccgagg agcgatcggc ccggcaccgg gccgagcacc
     6301 tggagcgcag cgtcgagggc ctgcggagtc ggctgggcgc ggccgtcgcc gaggccgacg
     6361 cggcccgacg ggcgctcgac gcggagcgct cccggcggcg ggagctggag cgcgaggtcg
     6421 aggctcaccg ccgtcggacc gaggccgcgg tcgccgaacg tgaccgcgcc accgcggagc
     6481 tggcagaggc ccggcggatc gcggacgatc ggctgtcgcg tgagcgctcc gcccgcgagc
     6541 tgttgagccg gttcgaggcg gagcgggacc gatgggtcgc accggtcgac caggccctcg
     6601 tcgacgcacg cgccgtgctc gacgagctgg ctgcggcgct cgacgtcgcc ggcgcggccc
     6661 tgcgtcgtgg c
//
LOCUS       DAOQOD010000169         6565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4519_length_6565_cov_13.016743, whole genome shotgun sequence.
ACCESSION   DAOQOD010000169 DAOQOD010000000
VERSION     DAOQOD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_6565_cov_13.016743"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1381)
                     /locus_tag="PLZ72_13305"
     CDS             complement(125..1381)
                     /locus_tag="PLZ72_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNA37321.1"
                     /translation="MARRLPSWIPRPLVLGRRLVDLLGPTGGAARQSLVALLFNSSTS
                     IVAGAVLGAITGTFEALPGLLVMVPAAIGLRGNIFGTFGNRLSTSIHTGTFRLSAKRE
                     SVLGQNVIASIALTFSLSLILAVVARIISVGLGVENAIGVLDLSLVSIAGGLLASLIV
                     LGATVLLSIGAVRRGWDMDNLVAPTTATLGDVITIPSLFLATKLLGHGAVTSALGWGL
                     VALTVVVFVTAIRSRLSELREIIWESLPVLTAAVVLSTLAGVAVQKQLAVFAALPALL
                     VMEPAFVSSAGALGGILSSRTSTNLHLGVVEPRINPGPVVRQDALLVFALGFPVFLFN
                     ALGAAGAAAVLGQDGPGLVWMVLLSLIGGAVTVAFVTALAYYSTMAAWRFDVDPDTYG
                     IPVVTATVDFVGVVILIVTMFALGIA"
     gene            complement(1538..2611)
                     /locus_tag="PLZ72_13310"
     CDS             complement(1538..2611)
                     /locus_tag="PLZ72_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNA37322.1"
                     /translation="MPSSQGFDPSSVHERLRSWLAAQLPDAENVEVSNLDAPSATGHS
                     SETVLFDASWTEAGSPHHESLVLRTAPSGHTVFPTYDLAEQFDVMTRVAAAAPTVPLP
                     PLRYHESDPDVLGGEFIVMGRVDGQVPPDRMPYTMEGWLLEASTPAEQRRLQDSAIDV
                     LADIHAIDWRGAGLDRLDRPELGRTGLDQQLRFYEDFLEWGRGDHPHERFVEVARWLR
                     ENRPDPEPEPVLNWGDARIGNIIFRDHRPAAVLDWEMATLGPRHVDVAWFCLFERFFS
                     VELGVANLPGFAPMDEVVARYEARSGTQLGDLGWYTAWGAYRYAVVMMRICQADALAG
                     IDGFPEDDNVATVMLDHVMDEVG"
     gene            complement(2687..4243)
                     /locus_tag="PLZ72_13315"
     CDS             complement(2687..4243)
                     /locus_tag="PLZ72_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNA37323.1"
                     /translation="MRPNVLLVTLDQFRADCLGVGGHPLVSTPTLDRLAGEGVRFTHH
                     FANCAPCAPGRASLLTGLWQMNHRVTDNGAPLADHLPTLPRALRSLGYRPTLFGYTDT
                     TYDPMTLSPGAPELREWEQPMRGFDLGVHLDDFIAPWLAWLADEGFDVPPAAEYFSIY
                     DPVEGAVPAGRGATWAPPRYDADHTESAFLTRRALEWLERELDTRGGAAAGDPWCAHL
                     SYLRPHPPYVVPAPYNDMFDPAQVPTPVRRADPKAEADLHPFVAAALGIVASPPDRVD
                     QDQLRATYYGMIAEVDHQLGVLLDGLDRLGQRDDTVVIVTSDHGEQLGDHWLVEKLGF
                     FDQSYRIPLIIRWPQAEARSGRVIDAFTESVDILPTVVDLCGGTPPPFCDGASLRPFL
                     DTDRSGTDAPERWRDCVHTEYDFRMPTSSMVQDTFGLRHDQCAVAVIRDRAGKYVEFG
                     GDLPPLFFDLSEDPDELVDLVSVPDRSGQVLDYAQRLLRLRIEHTDARLANHRATPFG
                     ARPGADPPRP"
     gene            4299..5231
                     /locus_tag="PLZ72_13320"
     CDS             4299..5231
                     /locus_tag="PLZ72_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37324.1"
                     /translation="MADPTGPDPAGTASGTTASGTAVPWPTGLGIVDTMFDLPFADIS
                     KVYEFLSPQLRDAQSRDDFEFPVEYMFKDVPKAALAERDPVDHALALMDHYGIETALI
                     GSGSEDQSRALSGHPDRFLPSMGVDPNDGMLAVEAIVRTHETIGLRAVAAFPAGCSPQ
                     VPIDDKRWYPVYAKCCELGIPVFMCVGVPGPRVPMGCQKVERLDEVCWFFPELKVVMR
                     HGAEPWEELAVKLMLKWPNLYYSTSAFAPRYYPKAIIDYANTRGADKVIYGGYFPMGL
                     TLERIFGDLPGVPLKEEVWPKFLRRNARRVLGLD"
     gene            complement(5280..>6565)
                     /locus_tag="PLZ72_13325"
     CDS             complement(5280..>6565)
                     /locus_tag="PLZ72_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNA37325.1"
                     /translation="DAAPAAFGTAGGVALARRAAVDSLVLLTNDGVLPWDPAESRSNG
                     PGPATVAVIGPASDDVRLLQGDYHYPAHLEIVYGNQSDDAGLLPQAGGAFSPGPHFPP
                     STTPLAAIRAAVDGTGTTVRHERGCDITGEDRGGIAAAVAAAADADLVVCCVGGRSGL
                     VPDCTVGEARDAVDLDLTGVQLDLIEAVAATGTPIVTVVVSGRIHTLARVEELSSATL
                     LAWVPGQEGGSAIADVIFGAVAPCGRLPVSLPRHVGQLPVHYNHRAGGGASAFWGDYS
                     DSPAAPLHPFGFGLSTTTFGYSDLSVDPGTTVAPTTVAVTVTNTGTRDGVEVVQLYAH
                     DETASVARPDRQLVGFARVPLVVGEARTVQFTLHPSRLAFFDDSFDFVCEPGSYRLEV
                     GGWAGSPALTAALDLGGDLEPYRQVEVVATTARIS"
BASE COUNT         1020 a         2226 c         2433 g          886 t
ORIGIN      
        1 cttgtagctg gtggtcgctg cggtggcgtg tgggctacaa gttcggcgac gggtgtcgaa
       61 cttgtagctg gtggtcgctg cggtggcgtg tgggctacaa gttcggcgac gggtgctgcc
      121 gcggtcaggc gatgcccaag gcgaacatcg tcacgatcag gatcaccacg ccgacgaagt
      181 cgaccgtcgc ggtcaccacc gggatcccgt aggtgtcggg gtcgacgtcg aagcgccagg
      241 cggccatcgt cgagtagtac gccagtgcgg tcacgaaggc gacggtgacc gcgccgccga
      301 tcaacgacag caggaccatc cacaccagcc cgggcccgtc ctggccgagg accgcggcgg
      361 cgcccgcggc gccgagcgcg ttgaacagga acaccgggaa cccgagggcg aacaccagca
      421 gggcgtcctg acggacgacc gggcctgggt tgatgcgggg ctccaccaca ccgaggtgga
      481 ggttggtcga cgtgcggctc gacaggatcc cgcccagggc cccggccgag ctcacgaacg
      541 cgggctccat gaccagcagg gcgggcagcg ccgcgaacac agcgagctgc ttctgcacgg
      601 cgacacccgc cagggtggac aggacgaccg ccgcggtgag caccggcagc gactcccaga
      661 tgatctcgcg cagctcgctc aggcgggagc ggatcgcggt gacgaacacc acgacggtca
      721 acgccaccag tccccacccc agcgccgagg tcacggctcc gtggccgagc agcttcgtgg
      781 ccaggaacag cgacgggatc gtgatgacgt cgccgagggt cgccgtggtc ggcgcgacca
      841 ggttgtccat gtcccaaccg cggcgcacgg cgccgatcga caacaggacc gtggcgccga
      901 ggacgatcag gctcgccagc agccctccgg cgatcgacac caacgacagg tcgaggaccc
      961 cgatcgcgtt ctccaccccc aaccccaccg agatgatccg ggcgacgacg gcgaggatca
     1021 acgacaggga gaaggtcagg gcgatcgagg cgatgacgtt ctggccgagg accgactccc
     1081 gcttggcgct cagccggaac gtaccggtgt ggatcgacgt cgagagccgg ttgccgaagg
     1141 tgccgaagat gtttccgcgc aacccgatgg cggccgggac catgaccagc aggccgggca
     1201 gcgcctcgaa ggtgccggtg atcgctccca gcaccgcgcc cgcgacgatg ctggtggacg
     1261 agttgaacag caacgccacc aggctctggc gcgcggcacc gccggtcgga ccgaggaggt
     1321 cgaccagtcg gcggcccagg accaggggcc gcgggatcca ggaggggagc cggcgggcca
     1381 tcgggcagaa cgtccctgcg cgtgatcggg ggcgaccgtc acggttcacg acgaggcggg
     1441 ctcgtccggg cgggccgcca ggcggaccgg ctcaccctag tggcggtcat cgggtccccc
     1501 gacgatgacc gaccgggtgt cgggcgacgg cagttgctca gccgacctcg tccatcacgt
     1561 ggtcgagcat gaccgtcgcc acgttgtcgt cctcggggaa gccgtcgatg ccggcgaggg
     1621 cgtcggcctg gcagatccgc atcatcacga ccgcgtagcg gtaggcgccc cacgcggtgt
     1681 accagccgag gtcgccgagc tgggtgccgc tgcgggcctc gtagcgagcg acgacctcgt
     1741 ccatcggggc gaagcccggc aggttggcaa ctccgagctc gaccgagaag aaccgctcga
     1801 acaggcagaa ccaggccacg tcgacgtggc ggggtccgag cgtcgccatc tcccagtcga
     1861 gcacggcggc cggccggtgg tcgcggaaga tgatgttgcc gatgcgggcg tcaccccagt
     1921 tgaggacggg ctcgggctcg gggtcgggtc ggttctcccg cagccagcgg gcgacctcga
     1981 cgaagcgctc gtgggggtgg tctccccggc cccactccag gaagtcctcg tagaagcgca
     2041 gctgctggtc caggccggtg cggccgagct cgggtcggtc gagtcggtcc agcccggcgc
     2101 cgcgccagtc gatggcgtgg atgtcggcca gcacgtcgat ggcgctgtcc tgcaacctcc
     2161 gctgctcggc cggggtggag gcctcgagca gccagccctc catggtgtac ggcatgcggt
     2221 ccggcgggac ctggccgtcg acccggccca tcacgatgaa ctccccgccg aggacgtcgg
     2281 ggtccgactc gtggtagcgc agtggtggca acggcaccgt gggcgcggcg gccgcgacgc
     2341 gggtcatgac gtcgaactgc tcggccaggt cgtaggtggg gaacacggtg tggccgctcg
     2401 gggcggtgcg cagcaccagc gactcgtggt gcggggaccc cgcctcggtc cacgacgcgt
     2461 cgaacagcac cgtctcgctc gagtgcccgg tggccgacgg agcgtcgagg ttggacacct
     2521 cgacgttctc cgcgtcgggc aactgggcgg ccagccacga ccgcagccgt tcgtgcaccg
     2581 acgacggatc gaaaccctgg ctgctcggca tctgtccccc tggaacctgg tccccgacgg
     2641 tccgacggga cggtgatccc ggcggtccgg acggtgcagc gtagcgtcag ggtcggggtg
     2701 gatccgctcc gggacgtgct ccgaacggcg tggcgcggtg gttggccagt cgggcgtcgg
     2761 tgtgctcgat gcgcagtcgc aacaggcgtt gggcgtagtc gagcacctgg ccggagcgat
     2821 caggaacgga cacgaggtcg acgagctcgt cggggtcctc ggacagatcg aagaacagcg
     2881 gtgggaggtc gccgccgaac tcgacgtact tgccggcccg gtcgcggatc accgccaccg
     2941 cgcactggtc gtggcgcaga ccgaaggtgt cctgcaccat cgacgacgtc ggcatccgga
     3001 agtcgtactc ggtgtgtacg cagtcccgcc accgttcggg tgcgtcggtc ccgctccggt
     3061 cggtgtcgag gaacggccgc agcgacgccc cgtcgcagaa gggcggcgga gtcccgccgc
     3121 agaggtcgac gacggtcggc aggatgtcga cgctctcggt gaaggcgtcg atcacccgac
     3181 ccgatcgggc ttccgcctgc ggccagcgga tgatcagcgg gatccggtag ctctggtcga
     3241 agaagccgag cttctccacc agccagtggt caccgagctg ctcgccgtgg tcggaggtga
     3301 cgatcacgac ggtgtcgtcc ctctggccga gccggtcgag gccgtcgagc aggacgccga
     3361 gctggtggtc gacctcggcg atcatgccgt agtaggtggc gcgcagctgg tcctggtcca
     3421 cgcggtccgg aggcgacgcg acgatgccga gcgccgcggc cacgaacggg tgcaggtcgg
     3481 cttcggcctt ggggtcggcg cggcggaccg gggtgggcac ctgcgccgga tcgaacatgt
     3541 cgttgtacgg agccggcacc acgtacgggg ggtgcgggcg caggtacgac aggtgcgcgc
     3601 accacgggtc gcctgcggcc gcgccgccgc gggtgtcgag ctcgcgctcg agccactcga
     3661 gggctcggcg ggtcaggaac gcggactcgg tgtgatcggc gtcgtagcga gggggtgccc
     3721 acgtcgcccc gcggccggcg ggcacggcac cctcgacggg gtcgtagatg gagaagtact
     3781 ccgcggcggg aggcacgtcg aagccctcgt cggcgagcca cgccagccac ggggcgatga
     3841 agtcgtcgag gtgcacgccc aggtcgaagc cgcgcatcgg ctgctcccac tcgcgcagct
     3901 ccggggcgcc cggcgacagg gtcatcgggt cgtaggtggt gtcggtgtag ccgaacaggg
     3961 tcggtcggta cccgagggat cgcagcgccc ggggcagggt ggggaggtgg tccgcgagcg
     4021 gagcgccgtt gtcggtcacc cggtggttca tctgccacag cccggtcagc agcgacgccc
     4081 gacccggcgc gcacggcgcg cagttggcga agtggtgggt gaagcggacg ccctcgccgg
     4141 ccaggcggtc gagcgtcgga gtgctcacca gcgggtggcc acccacgccc aggcagtcgg
     4201 cccggaactg gtcgagggtg acgaggagga cgttcgggcg caccggcgca agctaagcac
     4261 cgtcacgccc ggcggaggtg cccgggtacg gtgacccgat ggccgacccc accggacccg
     4321 accccgccgg cacagcatcc ggaacgacag catccggaac cgccgtgccg tggccgacgg
     4381 ggctcgggat cgtcgacacg atgttcgacc tccccttcgc cgacatctcc aaggtctacg
     4441 agttcctctc gccacagctg cgcgacgccc agtcgcgtga cgacttcgag ttcccggtcg
     4501 agtacatgtt caaggacgtc cccaaggccg cgttggccga gcgggacccg gtcgaccacg
     4561 cgctcgcgct catggaccac tacggcatcg agaccgctct gatcggctcg ggatccgagg
     4621 accagtcccg ggcgctgagc ggtcatcccg accggttcct cccgtcgatg ggcgtcgatc
     4681 ccaacgacgg catgttggcg gtcgaggcca tcgtccggac ccacgagacg atcggtctgc
     4741 gagccgtcgc ggcgttcccg gccggctgct cgccacaggt gccgatcgac gacaagcgct
     4801 ggtacccggt gtacgccaag tgctgcgagc tcggcatccc ggtgttcatg tgcgtcggcg
     4861 tgcccgggcc gagggtgccg atgggatgcc agaaggtgga gcgactcgac gaggtgtgct
     4921 ggttcttccc cgagctcaag gtggtgatgc gccacggcgc cgagccgtgg gaggagctcg
     4981 cggtcaagct catgttgaag tggccgaacc tctactactc gacttcggcc ttcgctcccc
     5041 ggtactaccc caaggcgatc atcgactacg ccaacacccg aggagccgac aaggtcatct
     5101 acggcggcta cttcccgatg ggcctgaccc tggagcgcat cttcggcgac ctgcccgggg
     5161 tgccgctcaa ggaagaggtg tggcccaagt tcctgcgcag gaacgcccgc cgggtcctgg
     5221 gtctggactg agctcgggga acgttgatcg cacaccggag cagggctctg gtgccgccat
     5281 caggagatcc gggcggtcgt ggcgaccacc tcgacctggc ggtacggctc gaggtcgcca
     5341 ccgagatcca gcgccgcggt cagcgccggc gatcctgccc aacccccgac ctcgagtcgg
     5401 tacgaccccg gttcgcacac gaagtcgaac gagtcgtcga agaaggcgag tcgtgacggg
     5461 tgcagggtga actgcaccgt ccgcgcctca cccacgacga gcgggacccg ggcgaatccg
     5521 accagctgcc ggtcgggccg ggccaccgac gcggtctcgt cgtgggcgta gagctgcacc
     5581 acctcgacgc cgtcacgggt gccggtgttg gtcaccgtga ccgccaccgt cgtcggtgcg
     5641 accgtggtgc ccggatcgac cgacaggtcg gagtagccga acgtcgtggt cgacagtccg
     5701 aagccgaacg gatgcagcgg ggcggccggt gagtcgctgt agtcacccca gaacgcgctc
     5761 gccccgccgc cggcccggtg gttgtagtgg accggcagct gcccgacgtg gcgggggagg
     5821 ctgaccggca gccggccgca gggagcaacc gcgccgaaga tcacgtccgc gatggccgag
     5881 cccccctcct gtcccggcac ccaggcgagc agcgtcgccg agctcagctc ctcgacccgg
     5941 gccaacgtgt ggatccgacc cgagacgacg acggtgacga tcggggtgcc cgtggcggcc
     6001 acggcctcga tcaggtcgag ctgcaccccg gtcagatcca ggtcgaccgc gtcgcgggcc
     6061 tcgccgacgg tgcagtcggg caccaggccg gagcgtcccc cgacgcagca gacgaccagg
     6121 tcggcgtccg ccgcagccgc gaccgcggcg gcgatgccgc cgcggtcctc gccggtgatg
     6181 tcgcatccgc gctcgtggcg gaccgtcgtc ccggtgccgt cgacagcggc gcggatcgcg
     6241 gccaatgggg tggtcgacgg cgggaagtgc gggccggggg agaacgcccc gccggcctgg
     6301 ggcagcagac cggcgtcgtc ggactggttg ccgtacacga tctcgagatg ggcggggtag
     6361 tggtagtcgc cctggagcag ccggacgtcg tcggacgccg gtccgatcac cgccacggtg
     6421 gccggtcccg gcccgttgct gcgactctcc gccggatccc acgggaggac gccgtcgttg
     6481 gtcagcagca ccagggagtc gaccgcggca cgccgggcca gggccacacc gccggcggtc
     6541 ccgaacgcgg ccggagcggc gtcgg
//
LOCUS       DAOQOD010000170         6528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4557_length_6528_cov_11.147536, whole genome shotgun sequence.
ACCESSION   DAOQOD010000170 DAOQOD010000000
VERSION     DAOQOD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_6528_cov_11.147536"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLZ72_13330"
     CDS             complement(<1..585)
                     /locus_tag="PLZ72_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37326.1"
                     /translation="MAADGPLDDRKSAILRAVVQQYIETAQPVGSTTVAHNPDVGVSS
                     ATVRNDMTVLEREGYLAQPHTSAGRVPTEKGYRFFVDGLADADTPSPTNVRAVRDFFA
                     TAHGEIESMLRETTRLLASLTHSAAVVVGDAPEVATLRSVQLVDLSPRTVLVVAVLSN
                     GVVLKRTFDPGAGLDGPAGDADLASARALIEATLV"
     gene            complement(662..2449)
                     /gene="lepA"
                     /locus_tag="PLZ72_13335"
     CDS             complement(662..2449)
                     /gene="lepA"
                     /locus_tag="PLZ72_13335"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNA37327.1"
                     /translation="MTRFDTIRNLSIIAHIDHGKSTLADRMLELCGAVDPRDMRAQYL
                     DSMDLERERGITIKLQSVRLEHRGHTLNLIDTPGHVDFGYEVSRSLAACEGVILVVDA
                     SQGIEAQTLANCYLALENDLEIVAVLNKIDLPAADPDTYAQEIENVLGIPAENVLRIS
                     AKTGAGVEDLLDAVIERIPAPEGDADAPLQGLIFDSHFDQYRGVVSSVRVVNGHLRTG
                     SRLRFMQAGAVHEAIEVGVRTPDNLAVSELGPGEVGYLIAGIKDVGEARSGETVTDNA
                     HPAGEPLAGYQDPKPMVFCGLYPVDGDEFEDLRESLEKLRLNDASFTYEPETSGALGF
                     GFRCGFLGLLHMEIIRERLEREYDLNLIATAPSVEYRVIRDDGEELVIDNPADLPDPG
                     TIEKVLEPYLRTTIITPTAYTGALMDLCQTRRGEMVKMEYLSPERVELQYRMPLGEVV
                     MDFFDQLKSRTQGYASLDYEPIGDEPADLVKVDILIAGDPVDAFSSIVHKDKAYDYGK
                     KMVDKLRELIPRQQFDVPIQAAIGSRILSRQTVKAYRKDVTAKLYGGDVSRKRKLLEK
                     QKEGKKRMKHLGRVEVPSDAFISALRLED"
     gene            2697..2954
                     /gene="rpsT"
                     /locus_tag="PLZ72_13340"
     CDS             2697..2954
                     /gene="rpsT"
                     /locus_tag="PLZ72_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNA37328.1"
                     /translation="MANIKSQIKRNRQNEKANERNRAVRSELRTRTKRVEAAVDAGED
                     ATELLKDAVKRIDQAAAKGVLHKNTAARRKARLVKKVNAAG"
     gene            complement(2938..4068)
                     /locus_tag="PLZ72_13345"
     CDS             complement(2938..4068)
                     /locus_tag="PLZ72_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37329.1"
                     /translation="MSGEIHLLKGSDPVLLNDAAVELVDRLVGDASRDEVLAEFSGDD
                     YDLGAVVLAAQTVSMFGDRVVVARNLARYGTAAKKGAAAADQGDDGAGDDGSGDGDPG
                     GPGPDAGSVAGSPADVQLLLDYLADPSPDASLVLVWSAPMTAGARRGAVPPKLTAAVK
                     AAGGTVSDHGLPQGRGTAMWLADHLAAASVTLDAGARKLIEDTLGEDMSRLPGVLRVL
                     EATFGSDAGRLSAEDIEPFLGDAGGVPPWDVTDAIDTGRVADAVTNVRRMVRGGGRHP
                     LAVMATLNTHYSRMLRLDGSGIRDEKSAAELLGMKKSTYPARKALDQGRKLGTDRLVR
                     AAGLLARADVDMRGRTAVPAEQVLEVLVARLAAQSAGVSRRR"
     gene            complement(4065..4391)
                     /locus_tag="PLZ72_13350"
     CDS             complement(4065..4391)
                     /locus_tag="PLZ72_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37330.1"
                     /translation="MEPQVVTESAYEALHPPVREANRSASLRERLAEVRRLAAEGTPV
                     ALHLDPADGPAVSVATAAVEAGASVLVLPGPASEEDAAPVALEREVRRAADVTAALVA
                     ARGAVR"
     gene            complement(4398..6050)
                     /locus_tag="PLZ72_13355"
     CDS             complement(4398..6050)
                     /locus_tag="PLZ72_13355"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNA37331.1"
                     /translation="MSDAHVVLGAVAVAVAAWLGASLPTPVVAAAVVMAVLRRRPLTV
                     LVALVAVTGLRSGDARSALESPLPARVAGTAQLVSDPRPGDHGSTVELRVSGRRYQAQ
                     VDRSDEWVLRPLLSGEHVEVVGRPRPFRGAPVGWQRSRHLAGRLVVTHIERGPPAAPW
                     FALANAVHRRLAAGVGSFDERARSLYLGLVVGDDRAQDDLARFRFQATGLGHLLAVSG
                     QNVAFVLLLVRPVASRVPLRVRWILGAGALVAFVLVTRAEASVLRAAAMALVALLAAT
                     SGRVTSGARMLALAVGALVLVDPLVVSGLGFQLSVAATVGLLVGVGPLVERVRGPRWF
                     SEAVATTVSAQLATAPLLMGLNGGVPSVATLTNVLAVPAAGAVMVLGLTAGVVAGFVV
                     DPAAAVLTAPARLLVGWIDGVAEVGSHSPLPLLGPLRLAALIVAAALAARRRGPTTSA
                     VTWARAVAAVVLVAVALWPVRAPSSATRVSPGVLVGDTVCGRTVQLEGNPDVSDTLAA
                     LQRAGVIHADVVVGVSARAVVPVATQLRADVAIGDDRGCRVA"
     gene            complement(6047..>6528)
                     /locus_tag="PLZ72_13360"
     CDS             complement(6047..>6528)
                     /locus_tag="PLZ72_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNA37332.1"
                     /translation="GALTTGSVTVHVAGAVQHPGVVTLPAGSRAVDAVDAAGGLAPGA
                     DADRVNLAAPLADGTRLAVPLVGQPIPTEVAVPSATPAGSGSGPTAATPGAPVDLNTA
                     DVTQLDTLPGVGPATAAAIVAHREEHGPFSTVDGLLDVRGIGEGRLDAVRDLVTVGR"
BASE COUNT          952 a         2397 c         2340 g          839 t
ORIGIN      
        1 gaccagcgtc gcctcgatga gggcgcgggc cgaggcgagg tcggcgtcgc cggccggacc
       61 gtcgagtcct gcaccggggt cgaaggtgcg cttgagcacc acgccgttgc tgagcacggc
      121 gacgaccagg acggtccggg ggctgaggtc gaccagctgc accgatcgca gcgtcgccac
      181 ctcgggggcg tcgcccacga ccaccgcggc gctgtgggtg agactggcca ggagccgggt
      241 cgtctcccgg agcatggact cgatctcgcc gtgggcggtg gcgaagaagt cccgaacggc
      301 ccggacgttg gtcggactgg gcgtgtcggc atcggcgagg ccgtcgacga agaaccggta
      361 gcccttctcg gtgggcaccc gaccggcgct ggtgtgcggc tgggccaggt agccctcccg
      421 ctcgaggacg gtcatgtcgt tgcgcaccgt cgcggaggag accccgacgt cggggttgtg
      481 cgcgacggtg gtcgaaccga ccggctgggc cgtctcgatg tactgctgca ccacggcgcg
      541 caggatcgcg gacttgcgat cgtcgagcgg tccgtcggca gccattggca ctcagtctac
      601 gcgagtgcca attcgggaca cgttccgggc aacatcccgc cctcgccgcc cgggaaggcg
      661 ctcagtcctc cagccggagg gcggagatga aggcatcgga gggcacctcg acacgaccga
      721 ggtgcttcat gcgcttcttg ccctccttct gcttctcgag cagcttgcgc ttgcgggaca
      781 cgtcgccgcc gtagagcttg gcggtcacgt ccttgcggta ggccttcacc gtctgacggc
      841 tgaggatgcg cgagccgatg gcggcctgga tcggcacgtc gaactgctgc cgcgggatca
      901 gctcgcggag cttgtcgacc atcttcttgc cgtagtcgta ggccttgtcc ttgtgcacga
      961 tcgagctgaa ggcgtcgacg gggtcccccg cgatgaggat gtcgaccttg accaggtcgg
     1021 cgggctcgtc gccgatcggt tcgtagtcca ggctggcgta gccctgcgtc cggctcttga
     1081 gctggtcgaa gaagtccatg accacctcgc cgagcggcat ccggtactgc agctcgaccc
     1141 gctccggtga caggtactcc atcttcacca tctcgccgcg gcgggtctgg cacaggtcca
     1201 tcagcgcacc ggtgtaggcg gtcggtgtga tgatcgtcgt ccggaggtac ggctcgagga
     1261 ccttctcgat cgtgccggga tcgggcaggt ccgccgggtt gtcgatcacc agctcctcgc
     1321 cgtcgtcgcg gatgacccgg tactcgaccg acggcgcggt ggcgatcagg ttcaggtcgt
     1381 actcgcgctc gaggcgctcg cggatgatct ccatgtgcag caggccgagg aagccgcacc
     1441 ggaagccgaa cccgagcgct ccggacgtct cgggttcgta ggtgaagctg gcgtcgttga
     1501 gacgcagctt ctcgagcgac tcccgcaggt cctcgaactc gtcgccgtcg acggggtaca
     1561 gaccgcagaa caccatcggc ttggggtcct ggtagccggc gaggggctct cctgccgggt
     1621 gggcgttgtc ggtgaccgtc tcaccggagc gggcctctcc gacgtccttg atgccggcga
     1681 tcaggtagcc gacctcaccg gggccgagct cggagaccgc caggttgtcc ggggtgcgca
     1741 cccccacctc gatcgcctca tgcaccgcgc cggcctgcat gaacctcagg cgggacccgg
     1801 tgcgcaggtg gccgttcacg acccggaccg agctgaccac gccgcggtac tggtcgaagt
     1861 gcgagtcgaa gatcaggccc tgcagcgggg cgtccgcatc gccctccggc gccgggatgc
     1921 gctcgatcac cgcgtcgagc aggtcctcga cccccgcacc ggtcttggcc gagatgcgca
     1981 gcacgttctc ggcggggatg ccgaggacgt tctcgatctc ctgggcgtag gtgtcgggat
     2041 ccgcggccgg gaggtcgatc ttgttgagga ccgccacgat ctccaggtcg ttctcgagcg
     2101 ccaggtagca gttggccagg gtctgcgcct cgatcccctg cgacgcgtcg accacgagga
     2161 tgaccccctc gcacgcggcg agggaccgcg acacctcgta gccgaagtcg acgtggccgg
     2221 gggtgtcgat caggttgagg gtgtggcccc ggtgctcgag gcgcaccgac tggagcttga
     2281 tcgtgatgcc ccgctcccgc tcgagatcca tcgagtcgag gtactgcgcc cgcatgtcgc
     2341 gggggtcgac cgcgccgcac agctcgagca tgcggtccgc caacgtggac ttgccgtggt
     2401 cgatgtgggc gatgatcgag aggttgcgga tggtgtcgaa tcgggtcatg gtgcgggatc
     2461 gtcggccggg acggggtgtc tgaccggggg tgccgggccg gggccggccc accagtctgg
     2521 cagcctcacc gggacgagcc gacatcaccg accgggcccc gggatgcgac accgtgcgcg
     2581 ccgcggctat cgttgcccgg tcgcccgcgg ggccactggc ccgcccgacc atccgcatcg
     2641 cacccccgcc gaccgcgcgt cggccaccat ccaccaggaa ctggacagca cccgtcatgg
     2701 cgaacatcaa gagccagatc aagcgcaacc gtcagaacga gaaggccaac gagcgcaacc
     2761 gcgccgtccg ctccgagctg cggacccgca ccaagcgggt cgaggccgcc gtcgacgccg
     2821 gtgaggatgc gaccgagctg ctcaaggacg ccgtcaagcg catcgaccag gccgcggcca
     2881 agggcgtgct gcacaagaac accgcggcgc ggcgcaaggc ccgcctggtg aagaaggtca
     2941 acgccgccgg ctgacgcccg cggactgggc ggccagccgg gccaccagca cctcgaggac
     3001 ctgctcggcg ggaaccgcgg tcctccccct catgtccacg tcggcccggg ccagcagccc
     3061 ggcggcccgc accagccggt ccgtgcccag cttgcgtccc tggtccagcg ccttgcgtgc
     3121 cgggtaggtc gactttttca tgccgagcag ctcggcggcg gacttctcgt cgcggatccc
     3181 ggatccgtcc aggcgcagca tccgcgagta gtgggtgttc agcgtcgcca tcaccgccaa
     3241 ggggtgccga cccccgcccc gcaccatgcg ccggacgttg gtcaccgcgt cggcgacgcg
     3301 accggtgtcg atggcgtcgg tcacgtccca gggcggcacg cccccggcgt cgcccaggaa
     3361 cggctcgatg tcctcggcgc tgagccggcc ggcatccgag ccgaacgtgg cctcgagcac
     3421 ccgcaggacg ccggggagcc gggacatgtc ctcaccgagg gtgtcctcga tgagcttgcg
     3481 ggccccggcg tcgagcgtga ccgacgccgc ggcgaggtgg tcggccaacc acatggcggt
     3541 gcccctgccc tggggcagcc cgtggtccga cacggtcccc ccggcggcct tgaccgccgc
     3601 cgtcagcttg ggtggcaccg ccccgcggcg ggcgcccgcg gtcatggggg cgctccagac
     3661 cagcaccagg ctggcgtcgg gggacgggtc ggccaggtag tcgagcagca gctgcacgtc
     3721 cgccggggat ccggccaccg atcccgcgtc cgggccaggc cccccggggt cgccatcacc
     3781 ggagccgtcg tccccggcac cgtcgtcgcc ctggtccgcg gcggccgcac ccttcttggc
     3841 ggcggtcccg tagcgggcca ggttgcgggc caccacgact cggtcgccga acatcgacac
     3901 cgtctgggcc gccagcacga ccgcacccag gtcgtagtcg tcgccggaga actcggccag
     3961 cacctcgtcg cgggaggcgt caccgaccag tcggtcgacc agctcgaccg cggcgtcgtt
     4021 caggaggaca gggtcgctgc ccttgagcag atggatctcg ccgctcatcg gaccgctcca
     4081 cgtgcggcga cgagggcggc ggtcacgtcg gccgcccgac gcacctcacg ctcgagcgcg
     4141 accggcgcgg catcctcctc ggacgcgggg ccggggagca ccaggacgct ggctccggcc
     4201 tcgaccgcgg ccgtggctac cgagacggcc gggccgtcgg cggggtcgag gtgaagcgcc
     4261 acgggcgtcc cctcggctgc cagtcgacgg acctcggcca gtcgctcccg gagcgacgcc
     4321 gagcggttcg cctcgcgtac cggcgggtgg agggcctcgt aggcggactc ggtgacgacc
     4381 tgcggctcca cgggcgctca agctacacgg cacccccgat cgtccccgat ggccacgtct
     4441 gctcggagct gggtggcgac cggcaccacc gcccgggcgg agacacccac gaccacgtcc
     4501 gcgtggatca ccccggcacg ctgcagcgcc gccagggtgt cggacacgtc cgggttcccc
     4561 tcgagctgca cggtgcgccc gcagacggtg tcaccgacga gcactcccgg agagacccgg
     4621 gtcgcgctcg acggtgcccg gacgggccac aacgccaccg cgaccaacac gacggccgcg
     4681 accgcccgcg cccacgtgac cgcggacgtg gtcgggcccc gccgccgcgc cgccagcgcc
     4741 gcggcgacga tcagcgccgc gagtcgcagc ggtccgagca gcggcaacgg cgagtgcgat
     4801 ccgacctcgg cgaccccgtc gatccagccg acgagcaggc gggccggagc ggtgagcacc
     4861 gcggcggcgg gatcgacgac gaagccggcg acgaccccgg ccgtgagccc gaggaccatg
     4921 accgccccgg cggcgggaac ggcgaggacg ttggtcagcg tcgccaccga cggcacgcct
     4981 ccgttgagcc ccatcagcag cggagccgtc gccagctggg cgctgacggt ggtggcgacc
     5041 gcctcggaga accagcgtgg cccacgcacc cgctcgacca acggtccgac gccgaccagc
     5101 agacccaccg tcgcggccac cgacagctgg aaccccagtc ccgacaccac cagcgggtcg
     5161 accagcacca gggcacccac ggccagggcg agcatccggg cccccgaggt gacccgaccc
     5221 gaggtcgcgg cgagcagcgc caccagtgcc atggccgcag cgcgcaggac cgacgcctcg
     5281 gccctggtca ccagcacgaa cgcgaccaac gccccggccc cgaggatcca tcggaccctg
     5341 agcgggaccc tcgacgccac cgggcggacc agcagcagga cgaacgccac gttctgaccg
     5401 ctgaccgcca ggaggtggcc cagtccggtc gcctggaacc ggaagcgcgc cagatcgtcc
     5461 tgggcgcggt cgtcgccgac gaccaggccg aggtacagcg aacgggcccg ctcgtcgaag
     5521 gaccccaccc cggcggccaa ccgacggtga acggcgttgg ccagagcgaa ccacggagcg
     5581 gcgggcggtc cccgctcgat gtgcgtgacg accaggcgcc cggccaggtg acgggaccgc
     5641 tgccacccga ccggcgcgcc gcggaacggg cgggggcggc ccaccacctc gacgtgctca
     5701 ccgctgagca gggggcgcag gacccactcg tcgctccgat cgacctgggc ctggtaccga
     5761 cggcccgaga cacgcagctc gactgtgctg ccgtgatcgc cgggacgggg gtccgacacc
     5821 aactgcgccg ttcccgccac ccgggcgggc aacggcgact cgagcgccga gcgggcgtcg
     5881 cccgagcgca gtcccgtcac ggccaccagg gcgacgagga ccgtcaacgg acggcggcgc
     5941 agcaccgcca tcacgaccgc ggcggccacg accggggttg gcagcgatgc tccgagccag
     6001 gccgccacgg cgaccgcgac ggcgccgaga accacgtggg cgtcgctcac cgaccgaccg
     6061 tgaccaggtc ccggacggcg tccagccgac cctcgccgat gcctcggacg tcgagcagcc
     6121 cgtcgaccgt gctgaacgga ccgtgctcct cgcggtgcgc gacgatcgcc gccgcggtgg
     6181 cgggcccgac ccccggcagg gtgtcgagct gggtcacgtc ggccgtgttc aggtcgaccg
     6241 gtgcgcccgg cgtcgccgcg gtcgggccgc tccccgagcc tgccggagtc gcggagggaa
     6301 ccgcgacctc ggtcgggatc ggctggccca cgaggggcac cgccagacgg gtcccgtcgg
     6361 cgaggggtgc ggccaggttc acccggtcgg cgtcggctcc gggggccagc ccaccggcgg
     6421 cgtcgaccgc gtcgacggct cggctgcccg ccggcaaggt caccacaccc gggtgctgca
     6481 ccgcgccggc gacgtgcacc gtcaccgatc ccgtcgtgag cgctccgg
//
LOCUS       DAOQOD010000171         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4595_length_6494_cov_11.994875, whole genome shotgun sequence.
ACCESSION   DAOQOD010000171 DAOQOD010000000
VERSION     DAOQOD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_6494_cov_11.994875"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLZ72_13365"
     CDS             <1..590
                     /locus_tag="PLZ72_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37333.1"
                     /translation="AAERPDDTAAERPDDTVAKRPDDTVAKRPDDTVAAEILLERWPS
                     ARPFVVVGAVAVVAGGAVAAVTRPTGFELGPWLAAFLVLVGGVAQIALGAGQAWVADR
                     LPDTGTVRAELTIWNIGLAATMAGSLLPAPLLTALGAVAVLIALALFLSATRPHHDAA
                     EPVAPRWARILYPLGIVVVLISTPIGLVLSWTRHG"
     gene            complement(650..1357)
                     /locus_tag="PLZ72_13370"
     CDS             complement(650..1357)
                     /locus_tag="PLZ72_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37334.1"
                     /translation="MTTLQQQARALGDPTRHAIFSLISESDRALGVSELTDRFALNHN
                     AIRQHLARLVDADLVVERKAPPVGRGRPPFEYVINPVAKDQWGNNGPYEQLSHLLAEI
                     ITTGQTPEEVGRRAADRLRVATPSGDVVADIGVAMARQGFDPEIREVDGGVDMVLHNC
                     PFATTAIDARDTVCSLHLGIAEGLVEGTDVHVEELVARDPARAECCLRIRVGAPEQDG
                     TGGSAGSGRLTLQRPAS"
     gene            1510..1878
                     /locus_tag="PLZ72_13375"
     CDS             1510..1878
                     /locus_tag="PLZ72_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNA37335.1"
                     /translation="MDVELERIYDAGPRTGPRHRVLVDRLWPRGISKAKADLDEWLKD
                     VAPSTELRRWYGHDVDLFDEFRRRYRDELGRPPAADALAALAETAEGDGVLLLTATRD
                     VEHSGAIVLEQVLTGLRGAR"
     gene            1893..2228
                     /locus_tag="PLZ72_13380"
     CDS             1893..2228
                     /locus_tag="PLZ72_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37336.1"
                     /translation="MPAETHQFIEDLDVDKVLELLVILVGVDAEPTDDPGGIDLGPLG
                     LDDDGAVLHLWDAVAEEYGERSVGELDLDDEPPATLGELAELFHLALRGDPEPGPPHD
                     GGAAPRQQR"
     gene            2225..>3453
                     /locus_tag="PLZ72_13385"
     CDS             2225..>3453
                     /locus_tag="PLZ72_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37337.1"
                     /translation="MSPEESPEPSGDDAGPTGARARTAARSATRSGRDRPDPADRVRR
                     SHAQTRTTIRFALAFFAAAALAAVWDHGRWLPLHLFLVGGVVLAISGVSLMLTVTWSA
                     APAPPDRWVTIQRTLIIVGAAGVGIGRTADVPAAVAVTGTAFLLGLLALAGLLAWTIR
                     RGPKRRFDAAVAAYLAAVAAGVVGVGLGVTMALDSPTLPLRSAHATANLLGLVGLTIG
                     GTLPFFAATVGRSKMARHTTPRRLVVTLAWQVAMVAAAVISLAVDAATVPSVALVGYA
                     VGILVILAWMPRPTRRQWTWAGPRLVALWLGGFWWCAAVVATAVDAATGTAVLDGRWL
                     VIMVLAGYGQIAWGSLAYLLPMLRGGGPDQLSDGFATTRSWPGLVAINVAGVAVAVAV
                     TPVAVAAVAVWLLDAAVR"
     assembly_gap    3454..3553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3595..4980
                     /locus_tag="PLZ72_13390"
     CDS             3595..4980
                     /locus_tag="PLZ72_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNA37338.1"
                     /translation="MGTYLLPAEPITSIDQYLATDVGGAGVEAAMRLGPAATIDAITE
                     SGLRGRGGGGFPTGRKWAGIAAQTGSRRYLVVNGAEGEPGTFKDRALLRANPYQVVEG
                     LIVAAFAVGAVEAYVGIKGSFEREIEAFTRAVQEFQTAGICTDCTVNIVAGPDEYLFG
                     EEKAMLEVIEGKPPLPRWFPPYEHGLFAASPQEGWEAGPHGPDRRTDEPNPTLVNNIE
                     TLANVAPILVHGTDWFRSMGTAESPGNVIVTVVGDVIAPDVGEIELGTPLGATIDAVG
                     SGVAQGRSVKAVFSGVANPVVTGAQLDTPVSYEGFAAIGSGMGSAGFVVYDDTACMVD
                     TAYQLSRFLAIESCGQCPPCKLGSGEITTHLERIQTGVGTDDDIARIGGWLERVTDGS
                     RCFLATEEQRLVSSVLRAFPEEFAAHIEQRSCPQERPRPMPKLVDLADGRATYDETFW
                     RKRPDWTYEPE"
     gene            complement(5020..5439)
                     /locus_tag="PLZ72_13395"
     CDS             complement(5020..5439)
                     /locus_tag="PLZ72_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNA37339.1"
                     /translation="MRDAPRPEPTRDLDSPEEIAEMVRRFYADVAQDDLLGPLFNDVA
                     RVDWSEHLPKLTAFWCRVLLGVPGYAGNPFRAHALVHQQVPFTPAHFLRWLGLFRETV
                     QTGWVGPTADGALELAANVARVHSQQLIGEPVELVGT"
     gene            5522..>6494
                     /gene="sufB"
                     /locus_tag="PLZ72_13400"
     CDS             5522..>6494
                     /gene="sufB"
                     /locus_tag="PLZ72_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNA37340.1"
                     /translation="MTDVIDGLVEREYAFGFESDIETERAPKGLNEDVIRLISAKKDE
                     PEWLLEWRLAAYRHFITMVEPDWAKVQHPPIDYQDMYYWAAPKPKGPAPKSLDEVDPE
                     IRATFDKLGISLAEQERLTGVAVDAIIDSVSVATTFKAKLAEVGVIFCSFSEAVREHP
                     DLVRKYLGTVVPPRDNFFAALNSAVFTDGSFCFIPAGVTCPMELSTYFRINAKETGQF
                     ERTLIIAEEGASVSYLEGCTAPMRDENQLHAAVVELIALDDSSIKYSTVQNWYPGDAE
                     GRGGIYNFVTKRARAGARAKVSWTQVETGSAITWKYPSVILQGDDSVG"
BASE COUNT          941 a         2360 c         2178 g          915 t
ORIGIN      
        1 ccgccgcgga acgacccgac gacaccgccg cggaacgacc cgacgacacc gtcgcgaagc
       61 gacccgacga caccgtcgcg aagcgacccg acgacaccgt cgcggccgag atcctgctcg
      121 agcgctggcc gtcggcccgc cccttcgtgg tcgtcggagc ggtcgccgtc gtggccggcg
      181 gcgcggtcgc ggcggtgacc cgtccgacgg ggttcgagct cggaccctgg ctcgccgcgt
      241 tcctggtgct ggtgggcggg gtggcccaga tcgccctcgg cgccgggcag gcgtgggtcg
      301 ccgaccggct ccccgacacc gggacggtcc gggccgagct gaccatctgg aacatcggcc
      361 tggcggcgac catggcaggg agcctgctcc cggccccgct gctcacggcc ctcggcgcag
      421 tggccgtgct gatcgcgctg gctctgttcc tgtccgcgac gcggccccac cacgacgcgg
      481 ccgagcccgt ggcgccccga tgggcccgga tcctctatcc gctcggcatc gtcgtggtgc
      541 tcatcagcac gccgatcggc ctggtcctgt cctggacccg ccacggctga ccgcgagcat
      601 cccgtcccgc atcatcctcg agcccttcgg atcgggcccg atcaggagct caggacgcgg
      661 gacgttgcag cgtcagccga ccgctccccg ccgatccgcc ggtgccgtcc tgttcgggag
      721 caccgacgcg gatccgcagg cagcactcgg cccgcgccgg atcccgggcg acgagctcct
      781 cgacgtgcac gtcggtgccc tcgaccaggc cctcggcgat accgaggtgc agggagcaga
      841 cggtgtcgcg ggcgtcgatc gccgtggtgg cgaaggggca gttgtgcaga accatgtcga
      901 caccgccgtc gacctcgcgg atctcgggat cgaacccctg gcgggccatg gccacgccga
      961 tgtcggccac gacgtcgccc gacggcgtgg ccacccgcag tcgatccgcg gcgcgccggc
     1021 cgacctcctc gggggtctga ccggtcgtga tgatctccgc cagcaggtgg ctgagctgct
     1081 cgtatggtcc gttgttgccc cactggtcct tggcgaccgg gttgatcacg tactcgaacg
     1141 gcggacgtcc gcgacccacc ggaggagcct tgcgctcgac caccaggtca gcatcgacca
     1201 gccgggccag gtgctgtcgg atcgcgttgt ggttcagcgc gaaccggtcg gtcagctcgc
     1261 tcacccccag agcgcggtcc gactcggaga tgagcgagaa gatggcgtgc cgggtcgggt
     1321 cccccagcgc ccgggcctgc tgttggagtg tggtcatggc tgctcccggt ggccggctct
     1381 cggaccgaac ggcacaactt ctagcctaga ctagagaaaa gaaagcacga cgtgaaggag
     1441 cctgcgagca ggacgccgcc atcacgacgg aagccggagg ctcgcagacg tagagtcgga
     1501 ggtgacccca tggacgtcga actcgagcgc atctacgacg ccggcccccg caccggtccg
     1561 agacaccgcg tgctcgtcga ccgcctgtgg ccccgaggga tctccaaggc gaaggctgac
     1621 ctcgacgagt ggctcaagga cgtggcgccg agcaccgagc tgcgccgctg gtacggccac
     1681 gacgtcgatc tcttcgacga gttccgccgc cgctaccgag acgagctcgg tcgaccgccg
     1741 gccgccgacg cgctggcggc gctcgcagag accgccgagg gcgacggcgt cctgctgctc
     1801 accgccaccc gcgacgtcga gcactcgggc gccatcgtgt tggagcaggt cctgaccggt
     1861 ctgcgcggcg cgcgctgaca ccggaggtca cgatgcccgc cgagacccac cagttcatcg
     1921 aggacctcga cgtcgacaag gtcctggagc tgctcgtcat cctggtcgga gtcgacgccg
     1981 agccaaccga cgatcccggc gggatcgacc tggggccgct cgggctcgac gacgacggcg
     2041 ccgtcctcca cctctgggat gcggtggccg aggagtacgg cgagcgctcg gtcggagagc
     2101 tcgatctgga cgacgagcca ccggccaccc tcggcgagtt ggccgagctg ttccacctgg
     2161 cgttgcgggg cgacccggag cccgggccgc cccacgacgg gggcgctgcg ccccgtcagc
     2221 aacggtgagt ccggaggaat cgcccgaacc ctccggcgac gacgccggcc ccacgggcgc
     2281 ccgcgcccgg accgccgccc gatccgccac ccgatccggt cgcgatcgac ccgatcccgc
     2341 cgaccgggtc cggcgatccc acgcccagac ccgcaccacg atcaggttcg ccctggcctt
     2401 cttcgctgcg gccgcgctgg ccgcggtgtg ggaccacgga cgctggctcc cgctgcacct
     2461 cttcctggtc ggcggcgtcg tcctggcgat cagcggggtg tcgctgatgc tgaccgtgac
     2521 ctggtcggcc gcgccggccc cacccgaccg ctgggtgacg atccagcgca cactgatcat
     2581 cgtcggcgcc gccggtgtcg gcatcggccg cacggcggac gttcccgcgg cggtcgcggt
     2641 gacgggaact gcgttcctcc tcgggctgct cgcactggcc gggctcctgg cgtggacgat
     2701 ccgacgggga cccaagcggc gcttcgatgc cgcggtcgcc gcctacctgg ctgccgtggc
     2761 cgccggcgtc gtcggcgtgg gcctgggcgt gaccatggcc ctggactccc ccaccctccc
     2821 gctgcggtcg gcgcacgcga ccgcaaacct gctcggcctg gtgggcctga ccatcggcgg
     2881 caccctgccc ttcttcgccg cgaccgtcgg acggtccaag atggctcgcc acaccacccc
     2941 ccgacggctg gtggtcaccc tggcctggca ggtggccatg gtggccgctg cggtcatctc
     3001 gctggccgtc gacgcggcga cggtcccgtc ggtcgccctc gtcggctacg ccgtcgggat
     3061 cctggtcatc ctcgcctgga tgccccggcc gacccggcgc cagtggacct gggccggccc
     3121 gagactggtg gcgctgtggc tgggcgggtt ctggtggtgc gccgcggtcg tcgccaccgc
     3181 ggtcgacgcc gccaccggca ccgcggtgct cgacggccgc tggctcgtca tcatggtgct
     3241 cgccgggtac gggcagatcg cctgggggtc gctggcatac ctgctgccga tgctgcgcgg
     3301 cggcgggccc gaccagctca gcgacggctt cgccaccacc cgatcctggc ccggcctggt
     3361 cgcgatcaac gtggccggcg tcgccgtggc cgtcgcggtg accccggtcg cggtggccgc
     3421 ggtcgccgtg tggctgctcg acgccgcggt gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnncaccgag acatccagca tcaggccatc caccgaggag tgacatgggc
     3601 acctacctgc ttcccgccga gccgatcacg tcgatcgacc agtacctggc gaccgacgtc
     3661 ggcggcgcgg gcgtcgaggc cgccatgcga ctcggacccg ccgccacgat cgacgccatc
     3721 accgaatcgg gcctgcgggg ccgcggcggc ggaggcttcc cgaccggccg caagtgggcg
     3781 gggatcgccg cccagaccgg gtcgcgccgc tacctggtcg tcaacggtgc cgagggcgag
     3841 ccggggacgt tcaaggaccg ggcgcttctc cgcgccaacc cgtaccaggt ggtcgagggg
     3901 ctgatcgtcg ccgcgttcgc ggtgggggcc gtcgaggcct acgtcggcat caaggggtcc
     3961 ttcgagcggg agatcgaggc gttcacgcgg gccgtgcagg agttccagac cgccgggatc
     4021 tgcaccgact gcaccgtcaa catcgtcgcc ggtcccgacg agtacctctt cggcgaagag
     4081 aaggcgatgc tcgaggtgat cgagggcaag cccccgctac cgcggtggtt cccgccctac
     4141 gagcacgggc tgttcgccgc gtccccccag gagggctggg aggccggccc ccacgggccc
     4201 gaccgacgca ccgacgaacc aaaccccacg ctggtcaaca acatcgagac cctggcgaac
     4261 gtggcgccga tcctggtcca cggcaccgac tggttccgtt cgatgggcac cgcggagtcg
     4321 cccggcaacg tgatcgtcac cgtcgtcggc gacgtcatcg ctcccgacgt cggcgagatc
     4381 gagctcggca ccccgctggg cgcgacgatc gacgccgtcg gcagcggcgt cgcgcagggt
     4441 cgatcggtca aggcggtgtt ctccggggtg gccaaccccg tcgtcaccgg agcccagctg
     4501 gacacgccgg tcagctacga gggcttcgcc gcgatcggca gcggcatggg ctccgccggc
     4561 ttcgtcgttt acgacgacac cgcctgcatg gtcgacacag cgtaccagct gtcacggttc
     4621 ctcgccatcg agtcctgcgg gcagtgccca ccgtgcaagc tcggttccgg agagatcacc
     4681 acgcacctcg agcgcatcca gaccggggtc ggcaccgacg acgacatcgc ccggatcggc
     4741 ggctggctgg agcgggtcac cgacggcagc cgatgcttcc tggccaccga ggagcagcgg
     4801 ctcgtctcca gcgtgctgcg ggcgttcccc gaggagttcg ccgcccacat cgaacagcgg
     4861 tcctgcccgc aggagcgacc ccggccgatg cccaagctgg tcgacctcgc cgacggtcgc
     4921 gccacctacg acgagacgtt ctggcgcaag cgccctgact ggacctacga acccgagtga
     4981 cccggccccc acggcgggga gccggaccca cgccgccgat caggtcccga cgagctcgac
     5041 cggttcgccg atcagctgct gggagtgcac ccgggccacg ttcgcggcca actccagcgc
     5101 gccgtccgcg gtcggcccga cccaccccgt ctggacggtc tcgcggaaca gtccgagcca
     5161 ccgcaggaag tgggcggggg tgaacggaac ctgctggtgg accagcgcat gggctcggaa
     5221 cgggttcccg gcgtatcctg ggactccgag caggacccga caccagaacg ccgtcagctt
     5281 gggaaggtgc tcggaccagt ccactcgggc cacatcgttg aacaaggggc cgagcagatc
     5341 gtcctgggcc acatcggcgt agaagcgacg gaccatctcg gcgatctcct cgggagagtc
     5401 cagatcccgg gtcggttccg gtcgtggcgc gtctcgcatg gcgggttccc ttctcggacg
     5461 attgtcactg ttttctctag ccgagactag atatattatc tcccacgaac gaggaggcca
     5521 catgaccgac gtgatcgacg gtctggtcga acgtgaatat gcgttcggct tcgagagcga
     5581 catcgagacc gagcgcgccc ccaagggcct caacgaggac gtcatccgac tgatctcggc
     5641 caagaaggac gagcccgagt ggctcctcga gtggcgcctg gccgcctacc ggcacttcat
     5701 caccatggtc gaaccggact gggccaaggt ccagcacccg ccgatcgact accaggacat
     5761 gtactactgg gccgccccca agcccaaggg acccgcgccc aagagcctgg acgaggtcga
     5821 ccccgagatc cgcgccacct tcgacaagct cgggatctcc ctcgccgaac aggagcgcct
     5881 caccggcgtc gccgtcgacg ccatcatcga ctcggtgtcg gtcgccacga ccttcaaggc
     5941 caagctcgcc gaggtcggcg tcatcttctg ctcgttctcc gaggcggtgc gcgagcaccc
     6001 ggacctggtg cgcaagtacc tcggaaccgt ggtgccgcca cgggacaact tcttcgccgc
     6061 gctcaactcg gcggtgttca ccgacggctc gttctgcttc atccccgccg gcgtcacctg
     6121 ccccatggag ctctcgacgt acttccggat caacgccaag gagaccggcc agttcgagcg
     6181 cacgctcatc atcgccgagg agggcgcgag cgtcagctac ctcgagggct gcaccgcccc
     6241 catgcgcgac gagaaccaac tccacgccgc ggtggtcgag ctgatcgccc tcgacgactc
     6301 gtcgatcaag tactcgacgg tccagaactg gtaccccggc gacgccgagg gtcggggcgg
     6361 catctacaac ttcgtcacca agcgggcccg cgccggcgcc cgggccaagg tctcctggac
     6421 ccaggtcgag accggctcgg cgatcacctg gaagtacccg agcgtgatcc tccagggcga
     6481 cgactccgtc ggcg
//
LOCUS       DAOQOD010000172         6459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4625_length_6459_cov_6.195034, whole genome shotgun sequence.
ACCESSION   DAOQOD010000172 DAOQOD010000000
VERSION     DAOQOD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_6459_cov_6.195034"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..807
                     /locus_tag="PLZ72_13405"
     CDS             343..807
                     /locus_tag="PLZ72_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37341.1"
                     /translation="MSGCRRPCRRGDGANGSSWEQLSARARTQAGFSLTESLAAILLV
                     TLVIMGIAAGILTTVRATRTVAQTQKSEAALSDATEVLKARPYQRCADLSTYGTIPDV
                     TVTTVEYLQAGAAGEQFAPTCDPATDTAQRITIVVGGRSADVVKRDPTAVTP"
     gene            804..2585
                     /locus_tag="PLZ72_13410"
     CDS             804..2585
                     /locus_tag="PLZ72_13410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA37342.1"
                     /translation="MTGPAELAHRPLGWIRRRLAQRGQAGLTLMEILLAMGIAAILMA
                     PLGTWAYGTLKSGIISQDELGRANATGLLNIYFLRDVASARAFVVTADGVTDCTGVPA
                     TAAGTPIVRLVQSGDVTTSVVYTVGNGPDPVPMFRHVCGPDGALTASTRVLAKVQASS
                     VSAFCSDAAGGPVGGCNDPAAVRVTMTLRQLSKAGPKAPITVSGTRRTTGTGTGFAQS
                     NPPVPTFKIDPGPRGYATTVFTATSTATDPDSGDTLALQWTLPAHAELISGGGTGPSV
                     TFRLPSSGEVVLTVSDGSNTASSAVPVWIVNRPPVFNPDWECRLVSERTYQLIGTAVD
                     PEVGPVAVQWFDPAGGAIAGGTWTAPDGLTGSIDLTMRASDGTDAVEQQVPCYVATPV
                     DAGIAITPTPTPGGVVNAVAPGGSRDVTFAAVDPGTASITWALYRKGAATPINSSASP
                     GASWTLTFLASEAGEYEVVRITDGTPGPRVGFRINVEPAVSLTSAEQTGVVPARIVTF
                     NSTATDTAPGSVVATTWDFGDGTVVEAPAGTVTHTYTAPGDYTVRFSATDNDGAVGAA
                     TLVINVPAAPGAGPGAPAPAAPIGGGG"
     gene            2582..5299
                     /locus_tag="PLZ72_13415"
     CDS             2582..5299
                     /locus_tag="PLZ72_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37343.1"
                     /translation="MSAITHALVSSVAAARRRRARAQGDQSGYLLIYVLGVIGLTSVL
                     VIALLGLALTSAKVAAMEARLARESRAADGALEAQVADIASRGGDVCGNSSTWPRTVE
                     ASGTGPEVEVTCDFGGVSDDPDADLPGPDVQVVGAGGAADSYQGGLLPAPSISGVADP
                     TVVVKASGHPAQFASDLFVAGGAAPNRTGGSPAVDVAGQYEQGAAVGGSGCGALAAAG
                     AHRIDDAGGQGEPTCGSVRPITAVAPVAVDAVPYVTGRAMTCPNVQLDPGAYLPADIT
                     RLNAILSGNEDCTVVFKPGGVHYLDVFDATRSGADRFVLTISNKNVKVIGGIPTSPLT
                     FPNACVNAPGSGGVQLVLSGRSAIRHTGGQVALCPDRTTGSALPVLQQSSSSDSQPRF
                     VSATSSSFANPDNLNPANSGWATTNVCGGTGPDDWFVWGGWLPRCRDRSFASTWSSTG
                     VGPLASAKVQLDMQQSPQLPPILWWAGPPWNPFDDPGSFHNVDLGVRFTVSGPWGSCT
                     TEDMKAGRSYSATLSYELRNNVAGNTCYGTLTDQAQLDGATVTATFFRFDVPGNGLVG
                     IRLGARNLRIETNSVAVAPTGAGAADAVWRTPANALVADNAVASVTQDAIGFTWLPGG
                     GVWVTPGTTTSMVLTGLDVPAELRSSDHLSRLGVTLRGPANQVSYLADPVDNGLTRVE
                     LVNAAGQTVCSTAEDTVTKAYTQSNQYLRYDLIAPGNCAGLRTVGQLDGLDLRLTFRV
                     GCAVLSLTNSPVYGPDGRCASVPLPKVDALSLSVGTDTVTGRPPYSSVTTNVADSTSF
                     DAFGPVALPLSDLDVRYTGTTSWPGGFSRPTFEGPLQVHGLGVEQAGTEPGVVCCVVG
                     RDAAVLVARVDGQVRAQAAVRVNPATTGSPTARRPVDVLSWKLCSRVGC"
     gene            5353..>6459
                     /locus_tag="PLZ72_13420"
     CDS             5353..>6459
                     /locus_tag="PLZ72_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37344.1"
                     /translation="MIRHGRHGGRRTWVALISLASAVVTLSGCLRTELAVNVDGDGSG
                     RVDLEVYFDELTLNESDLTTDDLAKLAETATQSIDGAEVSGVEAAGAKGVRLSIPFDD
                     YRQVTQSLTSGEVQGVSTRIFQTFDIAEGDEGHWTLNASLDPAGFRSILSQIPAGVAG
                     SLTQVDSSTEFVFSATLPGTVLRSNADSVDGGTARWNLDADAGPQTFTMESEPSSLST
                     LQKILIGVGALLVVGFILVFMTAAGSKRHHRRRGRKIKVQTNTPQDWAPTGPSGPVLM
                     PGEWSSPPPYSSHGWGEATPVGVPAEPPVADAGAVPVGGVPVAGAPVAGAVAVAPAIG
                     APVAAAPEGVAPVADAPVAGVSVAGAALAASPVAA"
BASE COUNT          889 a         2491 c         2188 g          891 t
ORIGIN      
        1 gctacgagca acagcgccgt tgtgggaggg gtgaagtttt ccatggggga ctgaagctca
       61 cccgccaccg ctcggttctg gcagcggtcg agatcaccag ccggcgcgtc tcgacctgaa
      121 gctgcctcga cctgaacgtg agctgtgtcc tcgacctcga cggcacctcg actcacaccg
      181 cgacctccac ctcgagctga accaggctcg gcgagaccgc gttgacaccc gcctcgtgga
      241 cgggaaggct gtcggcgacg tgtggcccac cttggggagc aggtcgcacg gtccgtgaca
      301 tcccggggca gatgtacggg tcgagggagg atcgtgcagc acatgagcgg gtgccggcgg
      361 ccgtgtcgcc gcggcgacgg cgcgaacgga tcgagctggg agcagctctc ggcgcgcgcc
      421 cgcacccagg ccggcttctc gctcaccgaa tcgctcgccg cgatcctgct ggtcaccctc
      481 gtcatcatgg gcatcgccgc ggggatcctc accaccgtgc gcgccacccg gaccgtggcc
      541 cagacccaga agtccgaagc cgcgctctcc gacgccaccg aggtcctcaa ggcccgcccc
      601 taccagcgct gcgccgacct cagcacctac ggcaccatcc ccgacgtcac cgtcaccacc
      661 gtcgagtacc tccaggccgg cgcggccggc gagcagttcg cgccgacgtg tgaccccgcc
      721 accgacaccg cgcagaggat caccatcgtg gtcggcggcc gctccgcaga cgtcgtcaag
      781 cgcgacccca cggcggtgac cccgtgaccg gcccggctga gctcgctcac cgcccgctcg
      841 ggtggatccg gcgccgcctc gcccaacgtg gccaggccgg cctcaccctc atggagatcc
      901 tgctcgccat gggcatcgcc gcgatcctca tggccccgct cggcacctgg gcctacggca
      961 ccctcaagtc ggggatcatc agccaggacg agctcggacg ggccaacgcc accggcctgc
     1021 tcaacatcta cttcctgcgc gacgtcgcct cggcccgggc cttcgtcgtg accgccgatg
     1081 gcgtcaccga ctgcaccggc gtgcccgcca ccgccgcggg caccccgatc gtgcggctcg
     1141 tgcagtccgg tgacgtcacc accagcgtcg tgtacaccgt cggcaacggc cccgacccgg
     1201 tccccatgtt ccgacacgtg tgcggacccg acggagcgct caccgcgtcg acccgcgtgc
     1261 tggccaaggt gcaggcgtcg tcggtcagcg cgttctgctc ggacgccgcg ggcgggcccg
     1321 tcggcggctg caacgacccc gccgcggtgc gggtcaccat gacgttgcgt cagctgtcca
     1381 aagccgggcc caaggcgccg atcaccgtca gcggcacccg ccgcaccacc ggcaccggca
     1441 ccgggttcgc ccagtccaac ccgccggtgc ccacgttcaa gatcgacccc ggcccccgcg
     1501 gatatgcgac caccgtgttc accgccacca gcaccgccac cgaccccgac agcggcgaca
     1561 cgctcgcgtt gcagtggacg ctgcccgcgc acgccgagct catcagcggc ggcggcaccg
     1621 gaccgtcggt gacgttccgg ctccccagct ccggcgaggt cgtcctcacc gtcagcgacg
     1681 ggtccaacac cgccagcagc gcggtgccgg tgtggatcgt caaccggccg ccggtgttca
     1741 accccgactg ggagtgccgg ctcgtctccg agcgcaccta ccagctgatc ggcaccgcgg
     1801 tggaccccga ggtcggcccc gtcgccgtcc agtggttcga ccccgccggc ggcgcgatcg
     1861 ccgggggcac ctggaccgcc cccgacgggc tgaccggttc gatcgacctg accatgcggg
     1921 ccagcgacgg caccgacgcc gtcgagcagc aggtgccctg ctacgtcgcc acccccgtcg
     1981 acgccgggat cgccatcacg ccgacgccca cccccggcgg cgtcgtcaac gccgtcgcac
     2041 ccggcggcag ccgcgacgtc accttcgccg cggtcgaccc cggcacggcg tccatcacct
     2101 gggcgctgta ccgcaaggga gccgccaccc cgatcaactc ctcggcctcg cccggcgcca
     2161 gctggacgct cacgttcctg gcatcggagg ccggcgagta cgaggtggtc cgcatcaccg
     2221 acggcacccc cggaccccgg gtcgggttcc gcatcaacgt cgaaccggcc gtcagcctca
     2281 ccagcgccga gcagaccggc gtggtcccgg cccggatcgt gacgttcaac tcgaccgcga
     2341 ccgacaccgc ccccggctcg gtggtcgcca ccacctggga cttcggcgac ggcaccgtgg
     2401 tcgaagcgcc cgccggcacc gtcacccaca cctacaccgc gccgggtgac tacacggtgc
     2461 ggttctccgc caccgacaac gacggtgcgg tcggcgccgc caccctggtg atcaacgttc
     2521 ccgccgctcc cggtgccgga ccgggtgctc cggcgccggc cgctccgatc ggagggggcg
     2581 gatgagcgcg atcacccacg ccctggtgtc gtcggtggcc gcggcccgcc gacgccgcgc
     2641 ccgcgcccag ggggaccagt ccgggtacct gctgatctac gtgctcggcg tcatcggcct
     2701 cacctcggtg ctggtcatcg ccctgctcgg cctggcgttg acctcggcca aggtcgcggc
     2761 gatggaggcc cgcctggccc gcgagtcccg ggccgccgac ggcgcactcg aggcccaggt
     2821 cgccgacatc gccagccggg gcggcgacgt gtgcggcaac tcctcgacgt ggccccgcac
     2881 cgtcgaggcg tccggcaccg gacccgaggt cgaggtgacc tgcgacttcg gcggcgtgtc
     2941 cgacgacccc gacgcggatc tgcccggacc cgacgtccag gtggtcggcg ccggcggcgc
     3001 cgccgacagc taccagggcg gcctgctgcc cgccccgtcg atctccgggg tggcggatcc
     3061 gaccgtcgtg gtcaaggcgt ccggacaccc ggcccagttc gcgtccgacc tgttcgtggc
     3121 cggcggcgcc gctcccaacc gcaccggcgg cagccccgcg gtcgacgtcg ccggccagta
     3181 cgaacagggc gccgccgtcg gcggaagcgg ctgcggagcg ctcgccgcgg ccggcgcgca
     3241 ccgcatcgac gacgccggcg gccagggcga acccacctgc gggtcggtgc gaccgatcac
     3301 cgccgtggca cccgtcgccg tcgacgccgt cccctacgtc accggccggg ccatgacgtg
     3361 cccgaacgtg cagctcgacc ccggcgccta cctgcccgcc gacatcaccc gcctcaacgc
     3421 catcctcagc ggcaacgagg actgcaccgt cgtgttcaag cccggtggtg tgcactacct
     3481 cgacgtgttc gacgccaccc gcagcggcgc cgaccggttc gtgctgacga tctccaacaa
     3541 gaacgtgaag gtgatcggcg ggatcccgac cagcccgctc acgttcccca acgcgtgcgt
     3601 caacgccccc ggctccggtg gggtgcagct ggtgctgtcg ggacgctccg ccatccgcca
     3661 caccggcggt caggtcgccc tgtgccccga ccgcaccacc ggcagcgccc tgccggtgct
     3721 gcagcagtcg tcgtcgtcgg actcccaacc ccggttcgtg tccgcgacgt cgagctcgtt
     3781 cgccaacccc gacaacctga acccggccaa ctcggggtgg gctaccacca acgtgtgcgg
     3841 tgggaccggc cccgacgact ggttcgtgtg gggtggatgg ttgccgcgct gtagggatcg
     3901 gagcttcgcc tccacgtggt catccaccgg tgtgggtccg ctggcgtcgg ccaaggtcca
     3961 gctcgacatg cagcagtcgc cgcagctgcc gccgatcctg tggtgggccg gaccgccgtg
     4021 gaatccattc gacgaccccg ggtcgttcca caacgtcgac ctgggggtcc gcttcacggt
     4081 gagcgggccg tggggaagct gcaccaccga ggacatgaag gcgggtcgct cctactcggc
     4141 caccctgtcc tacgagctgc gcaacaacgt ggccggcaac acctgctacg gcacgctcac
     4201 cgaccaggcc cagctcgacg gcgccaccgt gaccgccacg ttcttccgct tcgacgtccc
     4261 cggcaacggc ctggtcggca tccgtctcgg cgcccggaac ctgcggatcg agaccaactc
     4321 ggtcgccgtc gcccccaccg gtgccggggc tgccgacgcc gtgtggcgga cgccggccaa
     4381 cgcgctggtg gccgacaacg ccgtggcgtc ggtgacccag gacgccatcg ggttcacctg
     4441 gctgcccggc ggcggcgtgt gggtgacccc ggggacgacc acgtcgatgg tcctgaccgg
     4501 cctggacgtg cccgccgagc tgcgcagcag cgaccacctg agccgtctgg gcgtgaccct
     4561 gcgggggccg gccaaccagg tgtcgtacct ggccgacccg gtcgacaacg gactgacccg
     4621 cgtcgagctc gtgaacgccg cggggcagac ggtgtgctcg accgccgagg acaccgtcac
     4681 caaggcctac acgcagtcca accagtacct gcgctacgac ctgatcgccc ccggcaactg
     4741 cgccgggctg cgcaccgtcg gccagctcga cgggctcgac ctcaggctca cgttccgcgt
     4801 cggctgcgcc gtcctgtcgc tgaccaactc gccggtctac ggccccgacg gtcggtgcgc
     4861 cagcgtgccg ctgcccaagg tcgacgccct gtcgctcagc gtcggcaccg acaccgtcac
     4921 cggccgcccg ccctactcgt cggtgacgac gaacgtggcc gactcgacgt cgttcgacgc
     4981 gttcggaccg gtggccctgc cgctcagcga cctcgacgtc cgctacaccg ggacgaccag
     5041 ctggcccggc gggttcagcc gcccgacctt cgaggggccg ctgcaggtcc acggactcgg
     5101 cgtcgaacag gccgggaccg aacccggcgt cgtgtgctgc gtggtcggcc gtgacgccgc
     5161 ggtgctggtc gcccgggtcg acggacaggt gcgggcccag gccgcggtgc gggtcaaccc
     5221 cgccacgacc ggcagcccga ccgctcgtcg acccgtcgac gtgttgagct ggaagctctg
     5281 cagccgcgtc ggctgctgag gcgtggcgga ccaacggtgg agcggcgggg cgccgtggtc
     5341 cacactggag cgatgatccg acacggtcgg cacggcgggc gcaggacctg ggtggcgttg
     5401 atctcgctcg cgtccgcggt cgtgacgctc agcggatgtc tgcgcaccga gctggcggtc
     5461 aacgtcgacg gcgacggcag cggtcgcgtc gacctcgagg tgtacttcga cgagctcacc
     5521 ctcaacgagt ccgatctgac caccgacgac ctggccaagc tggccgagac cgccacccag
     5581 agcatcgacg gtgccgaggt gtcgggcgtc gaggccgccg gggccaaggg cgtccgcctg
     5641 tcgatcccct tcgacgacta ccgccaggtc acccagtccc tcaccagcgg cgaggtgcag
     5701 ggggtgtcga cgcggatctt ccagaccttc gacatcgccg agggtgacga gggccactgg
     5761 acgctcaacg cctcgctcga cccggcgggg ttccggtcga tcctgtcgca gatccccgcc
     5821 ggggtcgccg gttcgctcac ccaggtcgac tcgagcaccg agttcgtgtt ctcggccacc
     5881 ctgccgggga cggtgctgcg ctccaacgcc gattcggtcg acggcgggac cgcccgttgg
     5941 aacctcgacg cggatgccgg gccgcagacc ttcaccatgg agagcgagcc ctcgagcctg
     6001 tcgacgctgc agaagatcct gatcggggtc ggcgcgctgt tggtggtcgg gttcatcctc
     6061 gtgttcatga ccgccgcggg gtccaagcgc caccaccggc gccggggtcg caagatcaag
     6121 gtccagacca acacgccgca ggactgggcg ccgaccgggc cgtccgggcc ggtgctgatg
     6181 ccgggggagt ggagctcgcc gccgccgtac tcctcgcacg ggtggggtga ggccacgccg
     6241 gtcggcgtgc cggccgaacc gccggtcgcc gatgcgggag ccgttcccgt gggcggagtc
     6301 cctgtggctg gtgcgcctgt ggctggtgct gtggctgttg cgcctgcgat cggtgcgcct
     6361 gttgcggcgg ccccggaggg tgttgcgcct gtggcggatg cgcctgtggc gggtgtgtct
     6421 gtagccggtg ctgcgctagc ggcctctccg gtggcggcc
//
LOCUS       DAOQOD010000173         6452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4633_length_6452_cov_12.858527, whole genome shotgun sequence.
ACCESSION   DAOQOD010000173 DAOQOD010000000
VERSION     DAOQOD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_6452_cov_12.858527"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1477
                     /gene="purB"
                     /locus_tag="PLZ72_13425"
     CDS             56..1477
                     /gene="purB"
                     /locus_tag="PLZ72_13425"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNA37345.1"
                     /translation="MSVPNVLADRYASPEMAELWSAEHKIVLERRLWIAVMRAQRELG
                     IDIPDEAIDAYESVVDDIDLDSIAARERVTRHDVKARIEEFSDLAGHEHAHKGMTSRD
                     LTENVEQLQVRTGLELVRDRAVAALVRLAELAETHADTVIVGRSHNVAAQATTLGKRF
                     ANAGEELLVAVDRLEALIDRYPLRGLKGPVGTQLDQSELLDGDLDRLDDLEAAVARHL
                     GFERVLTNVGQVYPRSLDLEVVSALVQLLSGPSSLALTLRLMAGNELVTEGFRPGQVG
                     SSAMPHKMNARSCERINGFRVILNGHLTMAAGLAGEQWNEGDVSCSVVRRVVLPDAFF
                     AADGAFQTLIDVLDGFGAFPAVIERELRRFLPFLGTTKVLMAAVRAGVGRETAHEVIK
                     THAVATALDLRESGRDDNDLLDRLAGDARLGLDAAALRDALGDPASYAGNASAQARTF
                     VEQVAELARRHPDAATYEGAPVL"
     gene            1474..2382
                     /locus_tag="PLZ72_13430"
     CDS             1474..2382
                     /locus_tag="PLZ72_13430"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNA37346.1"
                     /translation="MSPLPLPHVHRGKVRDLYAVGEDRLLMVASDRLSAFDVVMAEPV
                     PDKGRVLTAMSAHWFAEIDDVIGNHLISTDVADLPAEVRRDDLAGRIMLCRRAEMLPV
                     ECIVRGFVAGSAWKEYRDRGTIHGMAAPAGLVEADRLPEPIFTPSTKAAVGDHDENIT
                     FDRAVEILGGDVADAAREASLAIFARASERAEAVGFLLADTKFELGWVPDPDGTPRLV
                     LADEVLTPDSSRYWAMEDWNPGATPQGFDKQPVRDYLESLGWDKLPPPPALPAEVVDA
                     TATRYRQAYERICGRSLDDWPGGPAS"
     gene            2456..2746
                     /gene="purS"
                     /locus_tag="PLZ72_13435"
     CDS             2456..2746
                     /gene="purS"
                     /locus_tag="PLZ72_13435"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNA37347.1"
                     /translation="MIFDVTVEVSLRPGIADPQGATIERALPALGFDGVSGVSVGKSI
                     RFRVDAADHATASALVDDLCHRFLSNPVIEDSTATISAVDAASGAAAVSGAV"
     gene            2755..3435
                     /gene="purQ"
                     /locus_tag="PLZ72_13440"
     CDS             2755..3435
                     /gene="purQ"
                     /locus_tag="PLZ72_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNA37348.1"
                     /translation="MKVAVVAFPGTNCEMDVVEAVRSLGAEAQVVFHDGDHLDGVDAV
                     VIAGGFAHGDYLRPGAIARFSPIMPAVGDFAAEGGPVVGICNGFQVLTEAGLLPGALQ
                     KNAGLKFRCETSELRVETTDSALTGEAEVGQVLRIPINHFEGNYTCSPETLAELRAED
                     RIVLRYVDNPNGSVDDIAGICNAGRNVVGLMPHPERAGHELLGSTDGRVLISSLLSAA
                     GARSAVSA"
     gene            complement(3535..3966)
                     /locus_tag="PLZ72_13445"
     CDS             complement(3535..3966)
                     /locus_tag="PLZ72_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37349.1"
                     /translation="MAAAPKSKKRVVSDEHKAAMAAGRTEARAVKNYLDALELTRPKR
                     GRKRTPDSINARLEKINAELDGGADPMKRLSLTQERIDLQKELDAMDSKVDVDMPALE
                     KAFIGAAASYSARKKISYAAWRENGVSAAVLKAAGISRAGS"
     gene            4254..>6452
                     /gene="purL"
                     /locus_tag="PLZ72_13450"
     CDS             4254..>6452
                     /gene="purL"
                     /locus_tag="PLZ72_13450"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNA37350.1"
                     /translation="MAAAEHTPRNGDAHDESSDGEPVHRALGLTDDELDRIRDTLGRE
                     PNHLELAMYSVMWSEHCSYKSSRIHLRRFPTEAPWVLVGPGEGAGVVDVGDGIAAALR
                     IESHNHPSAIEPYQGAATGVGGILRDIFSVGARPVAVMDPLRFGPLDDARSRWIAEGV
                     VSGVSGYGNSVGVPNVGGETVFDETYQGNPLVNVLCLGLMPTERLVLGRAEGVGNLAV
                     LLGSSTGRDGIGGVSVLASAGFGDDEADADKRPSVQVGDPFEEKRLIEACLELLDRDL
                     AVGVQDLGGAGITCATAETAAKGGAGMDVDVDAVPRREPHMAPFEVMTSESQERMLAI
                     VEPDKLDDVLAIARRWEVQATVIGTVTDSGRLRIVTAADGGGPEVVADVPAKSLEDEA
                     PLYDRPRSEPADLAVRREDSANSLPAPSDPGADLLDMLCDTSWVWSQYDHMLFLNTVI
                     APGGDAAVLRLKHPTTGVDTGRGMAMTTDGNHLWCAVDPRLGSTMTICESVMNLAVVG
                     ARPVAMVNCMNFGNPEHPEVMWQLSESVDGLSEALVAFGIPCIGGNVSLYNESRGADI
                     DPTPVVGLLGLIEPLERVPPRPRLVEGGALILVGDTVRELSGSRWAASQGHRGRGRLP
                     EVDLDAVSVAAGVTRELVAADLVLGAHDVSGGGLGLAVAEMAVASGVGATIARIADHV
                     DLFSESAGRAVLCVAPDRVRAVLDVLEAHGVAHSRIGEAGGDRIVVKDLLD"
BASE COUNT          907 a         2298 c         2265 g          982 t
ORIGIN      
        1 cagaacggtg ggggtgcgtt gtcgccgggg gtgcgcggtg ggcgatgatg aggcggtgag
       61 cgtccccaac gtcctggccg accgatacgc gagcccggag atggccgagc tgtggtccgc
      121 cgagcacaag atcgtcctgg aacgccggct gtggatcgcc gtcatgcggg cccagcggga
      181 gctcggcatc gacatccccg acgaggcgat cgacgcctac gagtccgtcg tcgacgacat
      241 cgacctggac tccatcgccg cccgcgaacg ggtgacccgt cacgacgtga aggctcgcat
      301 cgaggagttc tccgacctcg ccggccacga gcacgcccac aagggcatga ccagccgcga
      361 cctgaccgag aacgtcgagc agctccaggt gcgcaccgga ctcgagctcg tccgggaccg
      421 ggccgtcgcc gcgctggtcc ggctggccga gctggccgag acccacgcgg acaccgtcat
      481 cgtcggccgc agccacaacg tcgcggccca ggcgaccacc ctgggcaagc gcttcgccaa
      541 cgccggcgag gagctgctcg tcgccgtcga ccggctcgag gccctcatcg accgctaccc
      601 gctgcgcggg ctcaaaggcc ccgtcggcac ccagctcgac cagtccgagc tgctcgacgg
      661 cgacctcgac cgcctcgacg acctcgaagc cgcggtggct cgccacctcg gcttcgagcg
      721 cgtgttgacc aacgtcgggc aggtctaccc ccgctctctc gacctcgagg tcgtctccgc
      781 gttggtgcag ctgctgtcgg ggccgtcgtc gctggcgctc accctccgac tcatggccgg
      841 caacgaactg gtcaccgagg ggttccggcc cggtcaggtc ggttcgtcgg ccatgcccca
      901 caagatgaac gcccgcagct gcgagcgcat caacgggttc cgggtgatcc tcaacgggca
      961 cctcacgatg gcggccgggc tggccggcga gcagtggaac gagggtgacg tgtcgtgctc
     1021 ggtcgtgcgg cgcgtcgtgc tgcccgacgc gttcttcgcc gccgacggcg cgttccagac
     1081 cctgatcgac gtgctcgacg gcttcggggc gttcccggcg gtgatcgaac gggagctgcg
     1141 gcggttcctg ccgttcctgg gcaccaccaa ggtcctgatg gcggcggtgc gggccggtgt
     1201 cggccgggag accgctcacg aggtcatcaa gacccatgcc gtggccaccg ctctggacct
     1261 gcgcgaatcc ggtcgcgacg acaacgacct gctggaccgc ctggccggtg acgctcggct
     1321 cgggctcgac gccgcggctc tgcgcgacgc cctcggcgat ccggcctcgt acgcgggcaa
     1381 cgcctccgct caggcccgga ccttcgtcga gcaggtggcg gagctcgccc gacgacaccc
     1441 cgacgccgcg acctacgaag gggccccggt cctgtgagcc cgcttccgct cccgcacgtc
     1501 caccgcggca aggtccgtga cctctacgcc gtcggcgagg accgactcct gatggtcgcc
     1561 tcggatcggc tcagcgcctt cgacgtggtc atggccgagc ccgtgcccga caagggtcgg
     1621 gtgctcaccg ccatgtcggc ccactggttc gccgagatcg acgacgtcat cggcaaccac
     1681 ctgatctcga ccgacgtggc cgacctcccc gccgaggtcc gccgtgatga cctggccggt
     1741 cggatcatgc tgtgtcgccg cgccgagatg ctcccggtcg agtgcatcgt ccgcggcttc
     1801 gtggccggct cggcctggaa ggagtaccgc gaccggggca ccatccacgg catggcggcg
     1861 ccagccggac tcgtcgaggc ggaccggctg cccgaaccga tcttcacgcc gtccaccaag
     1921 gccgccgtgg gtgaccacga cgagaacatc accttcgacc gtgccgtcga gatcctcggc
     1981 ggcgacgtcg ccgatgccgc ccgggaggcc agcctggcca tcttcgcccg agcctcagag
     2041 cgggccgagg cggtcggttt cctgctggcc gacaccaagt tcgagctcgg ctgggtgccc
     2101 gatccggacg gcacgccccg actcgtgctg gccgacgagg tcctcacccc tgactcgtcg
     2161 cgctactggg ccatggagga ctggaacccg ggcgccaccc cacagggctt cgacaagcag
     2221 ccggtccggg actacctcga gtccctgggt tgggacaagc tccccccgcc gcctgccctg
     2281 cccgccgagg tcgtcgacgc caccgcgacc cgctaccgcc aggcctacga gcgcatctgc
     2341 ggccggtccc tcgacgactg gccgggcgga ccggcgtcgt gagcgaacgg atccaggtcc
     2401 cgaccggaca gctcgacgtc gggaaccgac ggctcggtgt gggagagttg gggtcatgat
     2461 cttcgacgtg accgtggagg tgtccctccg ccccggcatc gccgacccgc agggagcgac
     2521 gatcgaacgg gccctgcccg ccctcggctt cgacggggtg agcggggtgt cggtcggcaa
     2581 gtcgatccgg ttccgcgtcg acgccgccga tcacgccacg gcgtcggccc tggtcgacga
     2641 cctgtgccac cgcttcctct ccaaccccgt gatcgaggac tcgaccgcca cgatctcggc
     2701 ggtcgacgca gcctccggcg cagccgccgt gtccggggcg gtgtgatcgt ggcggtgaag
     2761 gtcgcggtcg tcgccttccc cggcaccaac tgcgagatgg acgtcgtcga agcggtccgc
     2821 tcgctcggcg cagaggcaca ggtcgtcttc cacgacgggg accacctcga cggcgtcgac
     2881 gcggtcgtca tcgccggcgg gttcgcccac ggcgactacc tgcgacccgg ggcgatcgcc
     2941 cggttctcgc cgatcatgcc cgccgtgggc gacttcgccg ccgagggcgg gccggtcgtg
     3001 ggcatctgca acgggttcca ggtcctgacc gaggccggtc tgctgcccgg ggccctccag
     3061 aagaacgccg ggctgaagtt ccgctgcgag acgtcggagc tgcgggtcga gacgaccgac
     3121 agcgctctga ccggggaggc ggaggtgggt caggtgctgc gcatcccgat caaccacttc
     3181 gagggcaact acacctgctc gcccgagacg ttggccgagc tgcgggccga ggaccggatc
     3241 gtgctgcgct acgtcgacaa ccccaacggc tccgtcgacg acatcgccgg catctgcaac
     3301 gccggacgca acgtggtggg gctgatgccg catccggagc gggccggcca cgaactgctc
     3361 ggttcgaccg acggtcgggt cctgatcagc tcgttgctgt ccgcggccgg tgcccgttcg
     3421 gccgtgtccg cctgagaggg agtcgccgcc cacccccggt cgttcgcggc gacggccgac
     3481 cccgatggtc ggccgtcgtc atgattcggg gatcccggtc gggatcgggg acgatcagga
     3541 tccggcgcgg gagatgccgg cagccttgag gaccgcggcg gacacgccgt tctcccgcca
     3601 ggcggcgtag gagatcttct tccgggcgct gtaggacgcc gcggcgccga tgaacgcctt
     3661 ctccaacgcg ggcatgtcga cgtcgacctt ggaatccatg gcgtcgagct ccttctggag
     3721 gtcgatgcgc tcctgggtga gcgacagccg cttcatcggg tcagcgccgc cgtcgagctc
     3781 ggcgttgatc ttctccaggc gggcattgat cgagtccggg gtgcgcttgc gtccccgctt
     3841 cggtcgggtg agctccagtg cgtcgaggta gttcttgacg gctcgggcct cggtccgacc
     3901 ggcggccatt gccgccttgt gctcatcgga gaccacgcgc ttcttggact tgggtgctgc
     3961 tgccatagtc ggcaattcca gcactatgag tgtggcaatt caagccaact cgaccggtct
     4021 gcgatcccga cgaccctccg ccggacgggt gcaatcgggc ggagtggatc atgggcgatg
     4081 aaccgagatg tgccgttcgt catgacgcgg atcgtcggtc gcggcgctgg tcaattcggg
     4141 ccagggtcat tgaagggtgt tcgaactgtt gcccgcacgg tgcttgtgcg catgatcgat
     4201 ccgactgaac ctccatttcg agggcgtatc ccaccgggca gtagtttgcg gtcatggccg
     4261 cagccgagca cacacctcgc aacggggacg cgcacgacga gagctccgac ggggagccgg
     4321 tgcaccgggc cctgggactg accgacgacg agctcgaccg gatccgcgac acgctcggac
     4381 gcgaacccaa ccacctcgag ctcgccatgt actcggtcat gtggtccgag cactgctcct
     4441 acaagagctc gcggatccac ctgcgccggt tcccgaccga ggcgccgtgg gtgctggtcg
     4501 gaccgggcga gggcgcgggg gtggtcgacg tcggcgacgg gatcgccgcc gcgctgcgca
     4561 tcgagagcca caaccacccc tcggccatcg agccctacca gggggcggcc accggcgtgg
     4621 gcgggatcct ccgggacatc ttctcggtcg gggcccgtcc ggtcgcagtc atggatccgc
     4681 tgcggttcgg tcccctcgac gacgcccgct cacgctggat cgccgagggc gtcgtgtcgg
     4741 gcgtgtccgg ctacggcaac tccgtcggcg tccccaacgt cggcggcgag acggtgttcg
     4801 acgagaccta ccagggcaac cccctggtca acgtcctctg cctcggtctg atgccgaccg
     4861 agcggctggt cctcggccgc gccgagggcg tcggcaacct ggcggtcctg ctcggatcca
     4921 gcaccggtcg tgacggcatc ggcggagtgt cggtcctggc cagcgccggc ttcggcgacg
     4981 acgaggccga cgccgacaag cgtcccagcg tgcaggtcgg cgatccgttc gaggagaagc
     5041 ggctgatcga ggcgtgcctg gagctcctcg accgcgacct ggcggtcggc gtgcaggacc
     5101 tcggcggtgc cggcatcacc tgcgccaccg ccgagacagc cgccaagggc ggcgccggca
     5161 tggacgtcga cgtcgacgcc gtgccccgtc gcgagccgca catggcgccg ttcgaggtga
     5221 tgacctcgga gtcccaggag cggatgctcg ccatcgtcga gcccgacaag ctcgacgacg
     5281 tcctggccat cgcccgtcga tgggaggtgc aggccacggt gatcggcacc gtgaccgaca
     5341 gcggtcgcct ccgcatcgtc accgccgctg acggcggtgg tcccgaggtc gtggccgatg
     5401 tgccggccaa gtcgctcgag gacgaggcgc cgctgtacga ccgtccccgg tccgagccgg
     5461 cggacctggc cgttcgacgg gaggactcgg ccaacagcct gcccgcgccg tcggatccgg
     5521 gagccgacct gctcgacatg ctctgcgaca cgtcgtgggt gtggtcccag tacgaccaca
     5581 tgctgttcct caacaccgtg atcgcaccgg gcggcgacgc cgccgtcctt cgcctcaagc
     5641 acccgacgac cggcgtcgac accggtcgcg ggatggcgat gaccaccgac ggcaaccacc
     5701 tgtggtgcgc ggtggacccc cggctcggat cgacgatgac gatctgcgag tcggtgatga
     5761 acctggcggt ggtcggggcg cgcccggtgg ccatggtcaa ctgcatgaac ttcggcaacc
     5821 ccgagcaccc cgaggtcatg tggcagctgt cggagtcggt cgacggcctg tcggaggccc
     5881 tggtcgcctt cgggatcccc tgcatcggcg gtaacgtcag cctctacaac gagagccggg
     5941 gggccgacat cgacccgacc ccggtcgtcg ggctcctcgg tctgatcgaa ccgctcgaac
     6001 gggtgccgcc ccgcccccga ctcgtcgagg gcggcgcgtt gatcctggtc ggcgacaccg
     6061 ttcgcgagct gtcgggttcg cggtgggcgg cgtcgcaggg ccaccgtggc cgcggccgcc
     6121 tgcccgaggt cgacctcgac gccgtgtcgg tcgcggccgg cgtcacccgg gagctggtcg
     6181 ccgccgatct ggtgctcggc gcgcacgacg tctccggtgg cgggctgggc ctggcggtgg
     6241 cggagatggc ggtggcctcg ggcgtcggag cgacgatcgc ccggatcgcc gatcacgtgg
     6301 acctgttctc cgagtccgcc ggacgtgcgg tgctgtgcgt cgcaccggac cgcgtgcgcg
     6361 cggtgctcga cgtcctggag gcccacggcg tggcccactc ccgcatcggc gaggccggag
     6421 gggaccggat cgtggtcaag gacctgctcg ac
//
LOCUS       DAOQOD010000174         6448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4639_length_6448_cov_10.861254, whole genome shotgun sequence.
ACCESSION   DAOQOD010000174 DAOQOD010000000
VERSION     DAOQOD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4639_length_6448_cov_10.861254"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..517
                     /locus_tag="PLZ72_13455"
     CDS             98..517
                     /locus_tag="PLZ72_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA37351.1"
                     /translation="MPLLPDEFAAHPARDASLASMTAIEAGDKDGWLALFADDAVVED
                     PIGPSPFSPDGLGQRGRAAIEAFYDSVIGPNDVSFRIDQSWAAGSEVANVGTITTRMA
                     DGTVVHTDGVFTYRVDDDGKVVALRAYWEMDRLRFGD"
     gene            655..2619
                     /locus_tag="PLZ72_13460"
     CDS             655..2619
                     /locus_tag="PLZ72_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA37352.1"
                     /translation="MTRTVITACPLDCPDACTLAVDVEGEATGGGERIVRIDAAPVTD
                     SNPMTDGWICAKVRRSTRRVHSPERILTPLRRTGPKGSGAFEPISWDDALELVATEIR
                     SAITEHGPAAVVPFLYNSSAPTFSDRLMVRLFAELGTSEVAHTICAYTAAIAWHSTFP
                     NMASADPLDVAHSDLVVIWGANPSTSNTHLTPLLTEARGRGAAVVVIDPRRTPTAARA
                     DRHLAVRPGTDDVLALATAAELDRLGLVDRSFCDVNAEGTDELLAAARAWTPSTAAEV
                     CGVDASAITELAELVGTRRPGLLRLGWGMERNRNGGSGIRAALGLWVLAGQFGRPGSG
                     VVTSTSSAAEDDTLDDLPDAPPRARTLNMNRIGHDLAHADPPVAVLIVQGANPAVSAP
                     DQQAVLAGLSREDLFTVVHDQVMTDTATFADVVLPATTYFEQGDDVAVGYTSYAASPL
                     RRVIAPVGESRTNGEVAAGLAAALGVDGYHPEQVPPPPLPERAVPTRPEGSVQFGPVG
                     EPGTTRPDGGRARLLLGVGSDPVPTHRPVDSPFPLALITPASSRQINSMFGEYDPPLA
                     QISMSPEDASARGVLDGADVRVFNDLATLVLPARIDPSVRPGVVSIPKGLWRRHLPGG
                     LTANALTPAEVEHTIGGAVFHDARVDIAPA"
     gene            complement(2779..3360)
                     /locus_tag="PLZ72_13465"
     CDS             complement(2779..3360)
                     /locus_tag="PLZ72_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNA37353.1"
                     /translation="MAFELPPLPYAQDALAPHISPETLEYHYGKHHQTYVTNLNKLVE
                     GTEFENASLEDVILKSDGGLFNNSAQVWNHTFYWNSMSPNGGGAPTGAVADAIDGAFG
                     SYDDFKAKFAEAATTQFGSGWAWLVDNGSGLEIMKTSNADLPMKHGAKALLTIDVWEH
                     AYYIDFRNARPNYISTFLDSLVNWDFVAQNLGS"
     gene            complement(3405..5906)
                     /locus_tag="PLZ72_13470"
     CDS             complement(3405..5906)
                     /locus_tag="PLZ72_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNA37354.1"
                     /translation="MPEVTPTPVDVATDTRTGLTAAQVAERVADGRVNRVPDDPSRTT
                     AQILKANVLTPVNAIVGVLLVLVVIADGIGPDMLFGLVIVANSVIGTVQEMRARAALE
                     RLAVLNTPHATVIRDGATSEHAVEEVVADDLLVLTSGAQVVVDGEVVSSAGLEIDESL
                     LTGESDPVHKAVGDTVMSGSFVSAGSGTFRATKVGADSYAASLAGAAREFSLVHSELR
                     TGVNRILEFLMVALPPIALILLWRLWRTSDDWREALSGTVAAGVAMVPDGLVLLTSLA
                     FMAGVVKLARRNALLKELASVEMLARVDTLCLDKTGTITTGQIVMAGTDLLPPPSPGF
                     LTTSHDNGGTPVSSESGGGGDVGEMLAALAASDPDPNATLAAIAHVHPDDPGWHRVDS
                     VPFSSVRKWSAAEFDGRGAIYLGAPEILAPGDDEVAARVQDQAVHGRRVVLVATAPRL
                     DGDQLPDDLVPRALVLLEDDVRDDARDTLAYLSGQGIDLKVISGDHPVTVGAVAARAG
                     VPDAGRVMDARDLPVDDDEALADAVAAHTVFGRVQPHQKRAMVRALQSRGHVVGMTGD
                     GVNDVLALKDADMGIAMGAGSAASRAVAQLVLLENRFSVLPGVLAEGRRVINSVERAA
                     NLFIYGTVYSACMAVVIAVVGTDYPFLPRHLTLVRSLSVGIPGFFLALAPDPRRARTG
                     FVARVVRFAVPAGIIAAAAALSVYFYARGPADVSLTDARSTATVTLLGVGLLLLLRLT
                     RTLPPWRWILVGAMGVGVLVVLAVPVAAEFFDLNHPPRSVWGAMALALVVAAVAIQFV
                     PVTADGGEVAELPDEFRAPSRARARGLAATGGDTG"
     gene            5990..>6448
                     /locus_tag="PLZ72_13475"
     CDS             5990..>6448
                     /locus_tag="PLZ72_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNA37355.1"
                     /translation="MTGRSSHPPRMHRPEDHKSRRAGWIRAMVLGANDGLLSTGALLL
                     GVAGAGATSSALLVAGVAALAAGALSMGVGEYVSVSSQLDVMRADQHMEATELKRHPE
                     AETRELTSIYQERGLSHDLAEQVAEALMAHDPLGAHMRDELGHTEEQRARP"
BASE COUNT          951 a         2359 c         2291 g          847 t
ORIGIN      
        1 cgccgccgaa cttgtagccc acacgccacc ccagcgacca ccagctacaa caacgccagc
       61 cgccgcgcga cggtgccggg ctggcagact cccgaccatg ccgctgctgc ccgacgagtt
      121 cgccgcccac cccgcccgcg acgcttcgct ggcgtcgatg accgcgatcg aggccggcga
      181 caaggacggg tggctggcgc tgttcgccga cgacgccgtg gtcgaggatc ccatcggacc
      241 atcgccgttc agccccgacg ggctcggcca gcgtggccga gccgccatcg aggcgttcta
      301 cgactcggtg atcggcccca acgacgtgtc gttccgcatc gaccagagct gggcggccgg
      361 ctccgaggtc gccaacgtcg gcacgatcac cacccgcatg gccgacggca ccgtcgtgca
      421 caccgacggc gtgttcacct accgcgtcga cgacgacggc aaggtggtcg cgctgcgggc
      481 gtactgggag atggaccggc tgcgcttcgg ggactgaacg gagaacgatc gggagtcgat
      541 cggagatcgg gaggcgccga ggcccgacag gcccggcgaa ccgaggttcg tcggtggcgc
      601 cccggcaccc gaaagtcggg gagcccacgg ccagggcggt gtcaggatgg atccatgacc
      661 cggaccgtga tcaccgcgtg cccgctcgac tgccccgacg cctgcaccct cgctgtcgac
      721 gtcgagggcg aggcgaccgg gggcggcgag cggatcgtcc ggatcgacgc cgctccggtc
      781 accgactcca acccgatgac cgacggctgg atctgcgcca aggtccgccg ttccacccga
      841 cgtgtgcact ccccggagcg gatcctcacc ccgctgcgac gcaccggccc caagggatcg
      901 ggtgcgttcg aaccgatcag ctgggacgac gctctggagc tggtcgccac ggagatccgc
      961 tcggcgatca ccgagcacgg ccccgcggcg gtcgtgccgt tcctctacaa ctcctccgcg
     1021 ccgacgttct cggaccggct catggtgcgc ctgttcgccg aactcggcac ctccgaggtc
     1081 gcccacacca tctgcgccta caccgcggcg atcgcctggc actcgacgtt cccgaacatg
     1141 gcgtcggccg acccgctcga cgtcgcccac tccgacctcg tggtcatctg gggcgccaac
     1201 ccctcgacgt cgaacaccca cctgacccca ctgctcaccg aggcccgcgg acggggtgcg
     1261 gcggtggtgg tgatcgaccc ccggcggacc ccgaccgcgg cccgagccga tcggcacctg
     1321 gcggtccggc ccggcaccga cgacgtgctc gcgctggcca ccgcggcgga gctcgaccgc
     1381 ctcggtctgg tcgaccgatc cttctgcgac gtcaacgccg agggtaccga tgagctcctc
     1441 gccgccgccc gggcgtggac cccgtcgacc gcggccgagg tgtgcggcgt cgacgcgtct
     1501 gcgatcaccg agctggccga gctggtcgga acccgacgcc ccggcctgct gcgcctcggt
     1561 tgggggatgg agcgcaaccg caacggcggg tccgggatcc gagccgcgct cgggctgtgg
     1621 gtgctggcgg gccagttcgg ccggccgggc tcgggcgtgg tcacctcgac gtcctccgcg
     1681 gccgaggacg acaccctcga cgacctcccc gacgcaccgc cccgcgcccg caccctcaac
     1741 atgaaccgca tcggccacga cctggcccac gcggatccgc cggtggcggt gctgatcgtc
     1801 cagggggcca accctgcggt gtcggccccg gaccagcagg cggtcctggc cggtctgtcc
     1861 cgggaagacc tgttcaccgt cgtgcacgac caggtgatga ccgacaccgc cacgttcgcc
     1921 gacgtcgtcc tacccgccac cacctacttc gagcagggtg acgacgtcgc cgtcggctac
     1981 acgtcctacg cggcgtcgcc tctgcgccgg gtcatcgccc cggtcggcga gtcgaggacc
     2041 aacggcgagg tggcggcagg gctggccgcc gcgctcggcg tcgacggcta ccaccccgag
     2101 caggttccgc cgccgccgct gcccgagcgg gcggtgccga cccggcccga gggcagcgtc
     2161 cagttcggac cggtgggcga accgggcacg accagacccg acggaggtcg ggcccggttg
     2221 ctgctcggag tggggtccga cccggtgccg acgcaccgac cggtcgactc gccgttcccg
     2281 ctggcgctga tcacacccgc gtcgagccga cagatcaact cgatgttcgg cgagtacgac
     2341 ccgccgctgg cgcagatctc gatgagcccc gaggacgcct cggcccgcgg ggtcctcgac
     2401 ggcgccgacg tccgggtgtt caacgacctg gccaccctgg tgctccccgc ccggatcgac
     2461 ccgtcggtgc gcccgggagt cgtgtcgatc cccaagggcc tctggcggag acacctgccc
     2521 ggcggcctga ccgccaatgc cctcacgcct gccgaggtgg agcacacgat cggcggggcg
     2581 gtcttccacg acgcccgggt cgacatcgcc cccgcctgac atcgcccccg cctgacacgg
     2641 ttggccgagc ctgacacggt tggccgacct gatcgccgac ccgccgaccg gacgcggatc
     2701 ggcccgcccc cacccagggc gggacggcgg gccgatcgac gatccgaacc ggtcggtccg
     2761 accggcgcgg actccgggtc aggaaccgag gttctgggcg acgaagtccc agttgaccag
     2821 gctgtcgagg aaggtcgaga tgtagttcgg gcgggcgttg cggaagtcga tgtagtaggc
     2881 gtgctcccac acgtcgatgg tgagcagggc cttggcgccg tgcttcatcg gcagatccgc
     2941 gttggaggtc ttcatgatct ccaggcccga gccgttgtcg accagccagg cccagccgga
     3001 gccgaactgc gtggtggccg cctcggcgaa cttggccttg aagtcgtcgt aggaaccgaa
     3061 cgcgccgtcg atggcgtcgg ccaccgcacc cgtgggcgct ccgccgccgt tcggcgacat
     3121 cgagttccag tagaaggtgt ggttccagac ctgcgcggag ttgttgaaca ggccgccgtc
     3181 ggacttgagg atcacgtcct cgagcgaggc gttctcgaac tcggtgccct cgaccagctt
     3241 gttgaggttg gtcacgtagg tctggtggtg cttgccgtag tggtactcga gcgtctcggg
     3301 cgagatgtgg ggcgcgagcg cgtcctgggc gtacgggagc ggcgggagtt cgaaggccat
     3361 gaggcgtcct ctcgaggaac ggggatgttg cttgcgcccc gaacctaccc cgtgtccccg
     3421 ccggtggccg ccagcccccg ggcccgggcc cgcgacgggg cccggaactc gtcgggcagc
     3481 tcggccacct cgccgccgtc cgcggtgacc gggacgaact ggatcgccac cgcggcgacg
     3541 accagggcga gagccatcgc cccccacacc gatcgcggcg ggtggttcag gtcgaagaac
     3601 tccgccgcga ccggaaccgc cagcacgacc agcaccccga cccccatggc accgacgagg
     3661 atccacctcc acggcggcag ggtccgggtc aggcgcagca gcagcaacaa ccccaccccg
     3721 agcagcgtga ccgtggcggt ggagcgggcg tcggtgagcg acacgtcggc tggcccgcgg
     3781 gcgtagaagt agaccgacag ggcggcggcc gcggcgatga tcccggccgg caccgcgaag
     3841 cgcacgactc gggccacgaa gccggtgcga gcacggcggg ggtcgggggc gagggccagg
     3901 aagaacccgg ggatgccgac gctgagggac cggaccaggg tcaggtggcg gggcaggaac
     3961 gggtagtcgg tgccgaccac ggcgatgacg acggccatgc acgccgagta caccgtgccg
     4021 tagatgaaca agttggcggc gcgctcgacg ctgttgatca cacggcgccc ctcggccagc
     4081 acgcccggca gcaccgagaa ccggttctcc agcaggacca gctgagcgac ggcgcggctg
     4141 gccgcggatc cggcgcccat ggcgatgccc atgtcggcgt ccttgagcgc cagcacgtcg
     4201 ttgacgccgt cgccggtcat gccgaccacg tggccccggg actgcagcgc ccgcaccatc
     4261 gcccgcttct ggtgcggctg cacccggccg aacacggtgt gggcggcgac agcgtcggcc
     4321 agggcctcgt cgtcgtcgac gggcaggtcg cgggcgtcca tgacccggcc ggcgtcgggc
     4381 accccggcgc gggccgcgac cgcgccgacg gtcaccgggt ggtccccgga gatgaccttg
     4441 aggtcgatgc cctggcccga caggtaggcg agcgtgtcgc gggcgtcgtc gcgcacgtcg
     4501 tcctcgagca ggaccaacgc tctcgggacc aggtcgtccg ggagctggtc cccgtcgagc
     4561 cgcggcgccg ttgcgaccag gaccacccgt cgaccgtgca cggcctggtc ctggacccgg
     4621 gcggccacct cgtcgtcgcc gggggcgagg atctccgggg cgcccaggta gatcgcgccc
     4681 cgaccgtcga actcggcggc cgaccacttg cggaccgagg agaagggaac cgagtcgacc
     4741 cggtgccagc ccgggtcgtc ggggtggacg tgggcgatgg ccgccagggt ggcgttgggg
     4801 tcggggtccg acgcggccag cgcggccagc atctcgccga catccccccc gcccccactt
     4861 tctgacgaca ctggagtacc gccattgtcg tgacttgtcg tcagaaaccc gggggagggt
     4921 ggggggagga ggtcggtgcc ggccatgacg atctgaccgg tggtgatggt gccggtcttg
     4981 tcgaggcaca gcgtgtcgac ccgggcgagc atctcgaccg acgccagctc cttgagcagc
     5041 gcgttgcgcc gggccaactt caccacgccg gccatgaacg ccaggctggt cagcagcacc
     5101 agcccgtcgg gcaccatcgc cacgccggcc gcgaccgttc ccgacagcgc ttcacgccag
     5161 tcgtcgctgg tgcgccacag ccgccacagc aggatcagcg cgatcggcgg cagggcgacc
     5221 atcaggaact cgaggatgcg gttgacgccg gtgcgcagct ccgagtgcac cagggagaac
     5281 tcgcgggcgg cgcccgccaa cgacgcggcg taggagtccg cacccacctt ggtcgcccgg
     5341 aacgtcccgg agccggcaga gacgaagctt cccgacatca ccgtgtcgcc gaccgccttg
     5401 tggaccgggt cggattcgcc ggtcaacagc gactcgtcga tctccaaccc cgcgctgctc
     5461 accacctcgc cgtcgacgac cacctgcgcg cccgaggtga gcacgagcag gtcgtcggcc
     5521 acgacctcct cgacggcgtg ctcggaggtg gcgccgtcgc ggatcacggt ggcgtgcggc
     5581 gtgttgagga ccgccaggcg ctccagcgcg gcccgggccc gcatctcctg caccgtgccg
     5641 atgaccgagt tggccacgat caccaacccg aacagcatgt ccggaccgat gccgtccgcg
     5701 atgacgacga gcaccaggag cacgccgacg atcgcgttga ccggcgtgag gacgttggcc
     5761 ttcaggatct gggcggtggt gcgcgacggg tcgtccggca cgcggttgac gcgaccgtcg
     5821 gccacccgct ccgcgacctg ggcggcggtg agcccggtcc tggtgtctgt cgccacgtcg
     5881 acgggggtgg gggtgacctc cggcatcgcc cggaggctac cggcggtgtg ccgctgaccg
     5941 accgacgtca cgtcgggcgc ggcaggccag cgggctaggt tcagcgccca tgaccggccg
     6001 gtcgagccac ccgcctcgga tgcaccgccc cgaggaccac aagagcagac gggccggctg
     6061 gatccgggcc atggtgctcg gcgccaacga cggcttgctg tccaccggcg ccctcctgct
     6121 cggggtggcc ggggcagggg cgaccagcag cgccctgctc gtcgccggcg tcgctgcact
     6181 ggctgccggc gcgctctcga tgggcgtggg cgagtacgtg tcggtcagct cccagctcga
     6241 cgtgatgcgc gcggatcagc acatggaggc gaccgagctg aagcgtcacc ccgaggccga
     6301 gacccgggag ctgacgtcga tctaccagga gcggggtctg tcccacgacc tggccgagca
     6361 ggtggccgag gcgctgatgg cccacgaccc gctcggcgcc cacatgcgcg acgagctcgg
     6421 ccacaccgag gagcagcgag cccgcccg
//
LOCUS       DAOQOD010000175         6412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4683_length_6412_cov_11.168948, whole genome shotgun sequence.
ACCESSION   DAOQOD010000175 DAOQOD010000000
VERSION     DAOQOD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_6412_cov_11.168948"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLZ72_13480"
     CDS             <1..523
                     /locus_tag="PLZ72_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37356.1"
                     /translation="CFAPAISVYAVDALVAANDRRPGDARAAAAIAGATVERLGDLSP
                     RTALLALILLARTAVAVGDRREARRLLGEAQEARRRDSSASALNVQLAELESCLGSGR
                     DLDRVDVEALTVAEQRVLELLPTHLPQGMIASELHLSRNTVKTHAAAIYRKLGVTSRA
                     DAVDVARSVGLLA"
     gene            593..1108
                     /locus_tag="PLZ72_13485"
     CDS             593..1108
                     /locus_tag="PLZ72_13485"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HNA37357.1"
                     /translation="MGLLRHRDDGPPAQRFQMRERLLSVGDDYWIEDGAGNRVFLVDG
                     KALRFRDTWILKDAGGADVAEIKERKLTVRDKMTIDLGGRSAHVTKRIVGIRDHFKVD
                     VDGESDLKVHGNIVDHEYEIERDGDQVAEVSKKWFRVRDTYGVEIAEGVDPVLILAVT
                     VAVDAMTRDVG"
     gene            1142..>3357
                     /locus_tag="PLZ72_13490"
     CDS             1142..>3357
                     /locus_tag="PLZ72_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNA37358.1"
                     /translation="MPATYPKLPAQENLPFPATPSASVAGRTMQDSTYQPREQPRRLP
                     EDAPNILVVLIDDAGPGLPSTFGGEVRTDNLTRVLSQGIGYNRFHTTAMCSPTRASLL
                     TGRNHHRIGNGQIAELANDWDGYSGHIPPSTALAAEVLTDYGYSTAAFGKWHNTPAEE
                     TTPSGPFHNWPTGKGFEYFYGFLAGEASQWEPNLVRNTTSVLPPKRPEEGYHLSEDLA
                     DDAIGWLRRHKAFEADKPFFMYWASGCLHGPHHIMKEWADRYAGQFDDGWDAYRERVF
                     DRAKAQGWIPADAELTPRDPTLAAWDDIPEAERPFQARLMEVAAGYAEYTDVQVGRLL
                     DEVETLGYGDNTLVVYIWGDNGSSGEGQNGTISELLAQNGIPTTVDMHIKALEELGGL
                     DVLGSPKTDNQYHAAWAWAGSTPYKGMKLLASHLGGTRNPMVVSWPGHIAPDARPRTQ
                     FHHCNDLVPTIYEILGITPPRTVNGVEQQPLDGVSFAYTFDDPDAEGRLRTQYFEIMG
                     SRSIYHDGWMASAFGPRSPWVPGMPPGILEWTPDQDTWELYHLDEDWTQSRDLADEHP
                     DKLAELKEVFAIEAARNSVYPIGGGLWVPVFHPEMRISTPYREWSFTGDIVRMPEFCA
                     PALGNRANTVDIDLECGDAASGVLYALGANSGGLTCYMDDGHLCYEYNLFILQRTKAR
                     SEAPLGAGRHSIRVETVYTEARPGGPLAVTLSVDGDVVATAAVPISAPLLFTANDCL"
     assembly_gap    3358..3457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3458..4123
                     /locus_tag="PLZ72_13495"
     CDS             <3458..4123
                     /locus_tag="PLZ72_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNA37359.1"
                     /translation="TAYGVLIGFSILLLFGQFADARHAVGDEATSVGTAFEQAALFPD
                     SAPAVQTALVCYAEAAARYDWPAMRADGGAPEVDRAFANLVQSLGVDDAPSTGALHSA
                     TATNLASQVGAISTARETRLVAAEADMPLMMWGLLVGGGLFVLVLIFVVTSTATPRTQ
                     AGLVAAAGMFTIMMLLLVAALNSPFANGPGRISPRLITEAASTMRASPNATTIGRCPE
                     LGS"
     gene            complement(4282..5025)
                     /locus_tag="PLZ72_13500"
     CDS             complement(4282..5025)
                     /locus_tag="PLZ72_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNA37360.1"
                     /translation="MSPTDRNPRKKPTRRKDSPSSKASPIVHLYVLLDRSGSMASMAD
                     DVIGGFNRLLADQQADGADARFTLVQFDSEDPQEVVADAIPIAEMAPLDASTFVPRGG
                     TPLLDATARLIASGTARAGALASVGHPAEEVVFVSVTDGHENASREQSLAAVKRLVEE
                     RTAAGWTFVFLGAALDVYGEAGGLGVAVGNTQMFEASADGAALAFDSLSQGTRNMRGK
                     VRRAEQVDKTDFFEEGKPAESHRRRTTGR"
     gene            5218..5967
                     /locus_tag="PLZ72_13505"
     CDS             5218..5967
                     /locus_tag="PLZ72_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37361.1"
                     /translation="MDPTTSNPGSDPVGGTRVDSSGDPAAAVDGREVLDWLLSDQARA
                     FARRRLRAAGLGSSADHVDDVLGDAVVSVMRRLRSADPLVLDSAEAYGTTVIANQVRA
                     VVRGRDLLVDDDPAARLALDRTAEPPPFVVDADFEDDLRVALENLGGPPWLMSAALAL
                     LIFIGHPGSEPDDAPAPISGSRPDQALCWPALWLAGEHDLFPEPYGRDGTDTVARRRR
                     RARRIRAVQAQLERGHAHLMAVDRRDGGDRG"
     gene            5960..>6412
                     /locus_tag="PLZ72_13510"
     CDS             5960..>6412
                     /locus_tag="PLZ72_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37362.1"
                     /translation="MAEPAPWGVHPDQADELVIVDDGGSTRLDGPAFGPAGAIVVDLG
                     DGSEWWVDHADPDDFVGYEPGAAWRTGADLAAEPAVAALFGGDAALMLADRWETRSVV
                     DPDGTDPVGGRGPRMRTAPRGDPPGSVGDGLLRIDLASDPGSSPLARLA"
BASE COUNT          911 a         2281 c         2224 g          896 t
ORIGIN      
        1 gtgcttcgct ccggcgatct ccgtctacgc ggtcgacgcg ttggtcgcag cgaacgaccg
       61 ccgccccggc gacgcccggg ctgccgcggc catcgccggc gccaccgtcg agcgcctcgg
      121 cgatctgtcg ccgcgcaccg cgctgctcgc tctcatcctc ctggcccgga ccgcggtcgc
      181 ggtcggcgac cgtcgggagg cccgccggct gctcggcgaa gctcaggagg cacgtcgtcg
      241 ggactcatcc gcgagcgcac tcaacgtgca gctcgcggag ctggagtcct gtctcggttc
      301 ggggcgggac ctggaccggg tcgacgtcga ggccctgacg gtggccgagc agcgtgttct
      361 ggagctcctg ccgacccacc tgccgcaggg gatgatcgcc tcggagctcc acctgagccg
      421 caacacggtc aagacccacg ccgcggcgat ctaccggaag ctcggggtca cgtcgcgtgc
      481 cgacgccgtg gacgtggccc gctcggtcgg actgctggcc tgagggcggc cgacgtccgc
      541 tcaccccatt cgggcgatgg cgacggcgca gccccggacg agactggtgg ccatgggact
      601 cctacgacac cgagatgacg gcccgcccgc gcagcggttc cagatgcgcg agcggctgtt
      661 gagcgtcggc gacgactact ggatcgagga cggcgcgggc aaccgggtct tcctggtcga
      721 cggcaaggcc ctgcgcttcc gcgacacctg gatcctgaag gacgcgggcg gcgcggacgt
      781 ggccgagatc aaggagcgga agctcacggt gcgggacaag atgaccatcg acctgggtgg
      841 tcggtctgcg cacgtgacca agcggatcgt cgggatccgc gatcacttca aggtcgatgt
      901 cgacggcgag tccgacctga aggtccacgg caacatcgtc gaccacgagt acgagatcga
      961 acgcgacggc gaccaggtcg ccgaggtgtc caagaagtgg ttccgggtgc gcgacacgta
     1021 cggcgtcgag atcgcggagg gagtcgaccc ggtcctgatc ctcgccgtca cggtcgcggt
     1081 cgacgccatg acccgcgacg tcggctgaac gcaaccggat cgaaccgagg agagcgcgct
     1141 gatgcccgcc acgtacccga agcttccggc ccaggagaac ctgccgttcc cggccacgcc
     1201 atcggcgtcg gtcgcggggc ggaccatgca ggactccacc taccagcccc gcgagcagcc
     1261 ccgccggctg cccgaggacg caccgaacat cctcgtggtg ctgatcgacg acgccggacc
     1321 gggcctgccg tcgaccttcg gcggagaggt gcgcaccgac aacctgaccc gcgtgctctc
     1381 ccagggcatc ggctacaacc ggttccacac caccgccatg tgctcgccga cgcgggcgtc
     1441 gctcctcacc gggcgcaacc accaccgcat cggcaacggc cagatcgccg agctcgccaa
     1501 cgactgggac ggctactcgg gccacatccc gccgagcacc gcgctcgcgg ccgaggtgct
     1561 caccgactac ggctactcga ccgcggcgtt cggcaagtgg cacaacaccc ccgccgagga
     1621 gaccaccccg tcgggcccgt tccacaactg gccgaccggg aagggcttcg agtacttcta
     1681 cggcttcctg gccggcgagg cgtcgcagtg ggagccgaac ctggtgcgca acaccaccag
     1741 cgtgctgccg cccaagcgcc ccgaggaggg ctaccacctc agcgaggacc tggccgacga
     1801 cgccatcggc tggctgcggc gccacaaggc cttcgaggcc gacaagccgt tcttcatgta
     1861 ctgggccagc ggctgcctcc acggtccgca ccacatcatg aaggagtggg ccgaccgcta
     1921 cgcgggccag ttcgacgacg gctgggacgc ctaccgcgag cgggtgttcg accgcgccaa
     1981 ggcccagggg tggatccccg cggacgccga gctcaccccg cgggacccga ccctcgccgc
     2041 gtgggacgac atccccgagg ccgagcgtcc gttccaggcc cggctgatgg aggtcgcggc
     2101 cggctacgcc gagtacaccg acgtgcaggt cggccgcctc ctcgacgagg tcgagacgct
     2161 cggctacggc gacaacaccc tggtcgtcta catctggggc gacaacggct cgtcgggtga
     2221 gggccagaac ggcacgatca gcgagctgtt ggcccagaac ggcatcccga cgacggtcga
     2281 catgcacatc aaggcgctcg aggagctcgg cggcctcgac gtcctcggtt cgcccaagac
     2341 cgacaaccag taccacgccg cgtgggcgtg ggcgggcagc accccctaca agggcatgaa
     2401 gctgctggcg tcgcacctgg gcggaacccg caacccgatg gtggtgagct ggcccggcca
     2461 catcgccccc gatgcccggc cgcgtaccca gttccaccac tgcaacgacc tggtgccgac
     2521 catctacgag atcctcggca tcaccccgcc gcggaccgtg aacggcgtcg agcagcagcc
     2581 gctcgacggg gtcagcttcg cctacacctt cgacgacccc gacgccgagg gccggctgcg
     2641 cacccagtac ttcgagatca tgggcagccg gtcgatctac cacgacggct ggatggcatc
     2701 ggcgttcggg ccccgcagcc cgtgggtgcc ggggatgccg ccgggcatcc tcgaatggac
     2761 tcccgaccag gacacctggg agctgtacca cctcgacgag gactggaccc agtcccgcga
     2821 cctggccgac gagcaccccg acaagctcgc cgagctcaag gaggtgttcg cgatcgaggc
     2881 ggcgcgcaac agcgtctacc cgatcggcgg cggcctgtgg gtgccggtgt tccaccccga
     2941 gatgcgcatc tcgaccccgt accgggagtg gagcttcacc ggcgacatcg tccgcatgcc
     3001 cgagttctgc gccccggcgc tcggcaatcg ggccaacacc gtcgacatcg acctcgagtg
     3061 cggcgacgcc gccagcggcg tgctctacgc gctcggcgcc aacagcggcg ggctcacctg
     3121 ctacatggac gacggccacc tctgctacga gtacaacctg ttcatcctgc agcgcaccaa
     3181 ggcccgctcc gaggcgccgc tgggtgccgg ccgccattcc atccgcgtcg agaccgtcta
     3241 caccgaggcc cgacccggtg gcccgctggc ggtcacgctg tcggtcgacg gcgacgtcgt
     3301 cgccaccgcc gcggtcccga tcagcgcccc gctgctgttc accgccaacg actgcctnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc gcgtacgggg tgctgatcgg
     3481 cttctcgatc ctgctgctgt tcgggcagtt cgccgacgcg cgccacgccg tgggcgacga
     3541 ggcgacctcg gtcggcaccg cgttcgaaca agccgcgctg ttcccggact ccgcccccgc
     3601 ggtgcagacc gcgctggtct gctacgccga ggctgctgcc cgctacgact ggccggccat
     3661 gcgcgccgat ggcggcgccc ctgaggtcga ccgggccttc gcgaacctcg tgcagtccct
     3721 cggcgtcgac gacgcgccgt cgaccggagc gctgcactcg gccacggcga ccaacctggc
     3781 atcccaggtg ggcgccatct ccaccgctcg ggagacccgg ttggtggccg ccgaggccga
     3841 catgccgttg atgatgtggg ggctgctcgt cggtggaggc ctgttcgtcc tcgtgctgat
     3901 cttcgtggtc acctcgaccg cgacgccgcg cacccaggcc ggtctggtcg ccgcggccgg
     3961 gatgttcacg atcatgatgc tgctgctggt cgcggcgctg aactcgccgt tcgccaacgg
     4021 ccccggccgc atcagtcccc ggctgatcac cgaggcggcg tcgaccatga gagcgtcgcc
     4081 gaacgcgacg accatcggtc gctgccccga gctcggctcc tgacccgaac gctttgtagt
     4141 gcgagatcca ggcgttcgcc tggatctcgc actacagaac gccggcggat gccccggggt
     4201 cgagggtggc ccgggcccga cgggatgggg cggccgtcgg gcccgggtgc cgagcgcccc
     4261 tccaggggcg tggaggagcg ctcagcggcc cgtcgtgcga cgacggtgcg actcggccgg
     4321 cttcccttcc tcgaagaagt cggtcttgtc gacctgctcc gcccgacgga ccttgccccg
     4381 catgttgcgg gtcccctgtg acagcgagtc gaacgcgagg gccgcgccgt cagcggaggc
     4441 ttcgaacatc tgggtgttgc cgaccgcgac gccgaggcca ccggcctcgc cgtagacgtc
     4501 gagcgcggcg ccgaggaaca cgaaggtcca gcccgccgcg gtccgctcct cgaccagtcg
     4561 cttcaccgcg gccagggact gctcccggct ggcgttctcg tggccgtcgg tgaccgacac
     4621 gaacacgacc tcctcggcgg ggtggccgac cgaggccagc gcacccgccc gggcggttcc
     4681 cgacgcgatc agccgggcgg tggcgtcgag cagcggggtg ccgccacggg ggacgaaggt
     4741 ggaggcgtcg aggggcgcca tctcggcgat cgggatcgcg tccgcgacca cctcctgggg
     4801 atcctcgctg tcgaactgga ccagggtgaa gcgggcgtcg gctccgtcgg cctgctggtc
     4861 ggcgagcagt cggttgaagc cgccgatcac gtcgtcggcc atcgacgcca tcgatccgga
     4921 acggtcgagc aggacgtaga ggtggacgat cggcgatgcc ttcgagctcg gcgagtcctt
     4981 cctgcgggtg ggcttcttgc gggggttcct gtcggtgggg ctcatggccg tctcctccgg
     5041 gtgtgggtgc tggatggggt gtcccagcgg actggtggcc cagctgggcc ggcggtcgtg
     5101 gccgctcacg gggttcgagc ccgccgacgc cgagatgtga catcggggtc gcggatcggc
     5161 ggcgcgatgt cacatctcgg caccacactt ctcggacgga gtggaggtga ggtcccgatg
     5221 gatccgacga cgagcaaccc gggcagcgac cccgtcgggg ggactcgtgt ggacagctcc
     5281 ggcgacccgg cagctgcggt cgatggtcgt gaggtgctcg actggctgct gagcgaccag
     5341 gcccgggcgt tcgcccgacg tcggctgcgg gccgccggac tcggatcgtc ggccgaccac
     5401 gtcgacgacg tgctgggaga cgccgtcgtg tcggtcatgc gtcgcctccg ctcggccgac
     5461 ccgctcgtgc tcgactcggc cgaggcctac gggaccacgg tcatcgccaa ccaggtgcgt
     5521 gccgtcgtgc ggggccgcga cctgctcgtc gacgacgacc cggcggcccg cctcgcgctg
     5581 gaccgcaccg cggagcctcc gccgttcgtc gtggacgccg acttcgagga cgatctgcgg
     5641 gtggcgctgg aaaacctggg cggcccgccg tggctgatgt cggccgcgct ggcgctgctg
     5701 atcttcatcg gccacccggg cagcgaaccc gacgacgcgc cggcgccgat ctccggctct
     5761 cggcccgatc aggccctgtg ctggccggcg ctctggctgg cgggggagca cgacctgttc
     5821 ccggagccct acggccgaga cgggaccgac accgtcgccc ggcgccgccg tcgcgctcgg
     5881 cggatccgcg ccgtgcaagc ccagttggag cgtggccacg cccacctcat ggcggtggac
     5941 cgacgtgacg gaggcgaccg tggctgagcc cgctccctgg ggcgtccacc ccgatcaggc
     6001 cgatgagctc gtcatcgtcg acgacggcgg atcgacgcgt ctcgacggtc ccgcgttcgg
     6061 gccggccgga gcgatcgtcg tcgatctcgg cgacggttcc gagtggtggg tcgaccacgc
     6121 tgatcccgac gacttcgtcg gctatgagcc cggcgcggcg tggcgaacgg gagccgacct
     6181 ggccgcggag cccgccgtgg cggcgttgtt cggcggtgac gcggcgctga tgctcgccga
     6241 ccgttgggaa acacggtcgg tcgtggatcc cgacgggacc gacccggtag gcgggcgcgg
     6301 gccgaggatg cggaccgcac cgcgggggga tccgccgggg tcggtgggcg acggtctgct
     6361 gcggatcgat ctggcgtccg atcccggttc gtcaccgctg gcccggttgg cg
//
LOCUS       DAOQOD010000176         6371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_4732_length_6371_cov_7.260766, whole genome shotgun sequence.
ACCESSION   DAOQOD010000176 DAOQOD010000000
VERSION     DAOQOD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_6371_cov_7.260766"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1397)
                     /locus_tag="PLZ72_13515"
     CDS             complement(63..1397)
                     /locus_tag="PLZ72_13515"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNA37363.1"
                     /translation="MGDTARGAAGSATEPLIVANCSGFYGDRLSAMREMLDGGPVDVI
                     TGDYLAELTMLILGKDQLRDPSLGYARTFVTQIEQCLGDALERGVRIVSNAGGLNPTG
                     LAERIREVAAGLGLSPAIGVVDGDDLAPRSGELGFGGALTANAYLGGFGIARALAAGA
                     DVVVTGRVTDASVVVGPGIWRFGWGTDDHDALAGAVAAGHVIECGTHATGGNFSGFMD
                     LPRDARPLGFPIAELHADGSSVITKHPGTGGTVTIDTVTAQLMYEIQGPVYDNPDVRT
                     DLRSIELSGDGPDRVRISGVTGSAPPEQLKVATNTLGGHRNQMEFVLVGLDLDAKEAW
                     LREQMDAALADRRPAEVVWTRGPEPNPDADTEEGAARRVRCVVKDPSAEVVGKAFTAA
                     GVELALASYPGFTLTAPPGKPSPFGVYRPAYVDRDQVRERALVDGVEIGEAS"
     gene            complement(1397..2191)
                     /locus_tag="PLZ72_13520"
     CDS             complement(1397..2191)
                     /locus_tag="PLZ72_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HNA37364.1"
                     /translation="MSVLDDVLDDLTAEGDQLESWVTPLSDEQWRTPTPAAGWDVATT
                     IAHLAWTDEVVVVAATDPTGWEQLILEAMGDPEGFVDATAAAAATAPAADLLDRWRTA
                     RRRLPEVLRAHPEGEKVPWFGPPMSPASMATARFMETWAHGLDVADALGVTAEPTDRI
                     RHVAHLGVRTRDFAYATHSLEPPAEAFRVELRAPSGETWTWGPDGAVQSVIGPAYDFC
                     LLVTHRRHRDDLDLVATGADADRWLDIAQAFAGPPGSGRTPTRGAT"
     gene            complement(2188..2802)
                     /locus_tag="PLZ72_13525"
     CDS             complement(2188..2802)
                     /locus_tag="PLZ72_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37365.1"
                     /translation="MTVTRVPQEERSRVMRERLLDAAVECLIECGYSGTSTTLVSERA
                     GVSRGAQLHHFPSRADLLVAAVEHVAQARGDDLVDAARRVAPGDRRKVLRMLADHVTS
                     PVFDAALELWVAARTDPELLAAVAPLERRIGRQTHQLAVAALDADESVPGTRELIEST
                     LDMIRGLGLAKALSDDTRRRNRVLDRWAGVLDRELGPITEPEQP"
     gene            complement(2885..3547)
                     /locus_tag="PLZ72_13530"
     CDS             complement(2885..3547)
                     /locus_tag="PLZ72_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37366.1"
                     /translation="MALTERNRSALYQGLTTVIDEEAVEEMLSYFPARDVEEPATKEF
                     LRAETALLRTELKDDMTVLRTELKDEMAVLRIEFKDETAVLRTELKDEMAVLRTELKD
                     ETAVLRTELKDEMAVLRTELKDETALLRTELKDEMAVLRTELKDETAVLRTELKDETA
                     LLHTELKDDMAVLRTEMAAMELRIDDRMDERFRSMYGLTIGTNVATVVAVAMVIFAAL
                     RL"
     gene            complement(3690..4172)
                     /locus_tag="PLZ72_13535"
     CDS             complement(3690..4172)
                     /locus_tag="PLZ72_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37367.1"
                     /translation="MGRPLSTDTWRDAPRGRVRVLVECPPENTPDLVAEVLRREGYEV
                     RVCEGPVPHHACPLVATGACNLVSGADVVVNLFGSTDPVRREVLGAITDERRPPAVIT
                     EMTTPELERRTESDSWWFDRDRVHIVETPVTAATLIEAVETALRLRSKEPPVSCSGVG
                     "
     gene            complement(4322..>6371)
                     /locus_tag="PLZ72_13540"
     CDS             complement(4322..>6371)
                     /locus_tag="PLZ72_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNA37368.1"
                     /translation="HRGALADAAALIGPQPLPPRYAFDYWYSRYWAYTDRDLLDIAER
                     FDTMDVPVDVMVIDMDWHLPGWTGYSWDRRYFPDPTDTLERLHRRGLRVTLNVHPADG
                     VGPHEDRYPEVARAVGVDPASGERIPFAVTDPGFIEAYFRFLHHPEEDRGVDFWWMDW
                     QQGTTSAVEGLDPLPWLNELHWNDQQSRRPDRRPLVFSRWGGMGGGRYPIGFSGDTWS
                     TWDSLALQPWFTATAANVGYGFWSHDIGGHTGEPPSPELYTRWMQFGAFSPVLRTHAT
                     KDPAQDRRFWEFADPHRSVMIRAVHRRYELVPYLYGQCRRAVDTGVSALRPMYHDHPD
                     RDAAYVADGQYLLGDDLVVAPVVTPLDDDRMAPIRVWLPRGGWYDTALGETVTVRRAD
                     GEWFERRYLLDEVPVFAAAGAVIAGQRDAQRLDAPCYPHPVITAYPGGDGDGELYEDD
                     GVSPDYLRGDSVTVRLRQRVTDRARSVRIGPARGDYAGWERHRPIEVRIVAEAVPRSV
                     EVDGRALPMATAPDGTGIRAGRGYWYHDTAAASVVVRMPRIDLRATTTVRLVRDRDRP
                     RSIDRMLDGYPGLARRLDAVAERVGEVSAGYELHPEERLAVDLAQAASRVTRDPTTLG
                     AEVRRARSSLGHLETVLAEFQTAWGRAIALPFPEQRIQAVAVVAEARAILATTRSQFG
                     "
BASE COUNT          852 a         2236 c         2352 g          931 t
ORIGIN      
        1 gttgccgccc ttgtcgccgg agcgggcgtg cacgaacgtg cccagcggga cgcggctggt
       61 gctcatgagg cctctccgat ctcgacgccg tcgacgagcg cccgttcgcg cacttggtcg
      121 cggtcgacgt aggcgggccg gtagaccccg aacggcgacg gcttgccggg cggcgcggtg
      181 agggtgaacc ccgggtacga cgccagggcc agctcgaccc cggcggcggt gaacgccttg
      241 ccgaccacct cggcggaggg gtccttgacc acgcagcgca cccgccgggc ggcgccttcc
      301 tcggtgtcgg cgtctgggtt gggttcaggt ccgcgggtcc agacgacctc ggcggggcga
      361 cggtcggcga gcgccgcgtc catctgctcg cgcagccacg cctccttggc gtcgaggtcc
      421 aggccgacga gcacgaactc catctggttg cggtggccgc ccagggtgtt ggtggcgacc
      481 ttgagctgct ccggcggtgc tgagccggtg accccggaga tgcgcacccg gtcggggccg
      541 tcgccggaca gctcgatgct gcgcaggtcg gtgcgcacgt cggggttgtc gtagaccgga
      601 ccctggatct cgtacatgag ctgcgcggtg acggtgtcga tcgtgaccgt gcctccggtg
      661 ccggggtgct tggtgatgac gctggatccg tcggcgtgca gctcggcgat cgggaacccg
      721 agcggccggg cgtcgcgggg caggtccatg aaccccgaga agttgccgcc ggtcgcgtgg
      781 gtgccgcact cgatgacgtg acccgcggcg accgcgccgg cgagcgcgtc gtggtcgtcg
      841 gtgccccacc cgaagcgcca gatgccgggg ccgaccacga ccgacgcgtc ggtcacccgg
      901 ccggtgacca ccacgtcggc gcccgcggcg agcgcccggg cgatgccgaa tccgcccagg
      961 taggcgttgg cggtcagcgc acctccgaag cccagctcgc cggagcgggg ggccaggtcg
     1021 tcgccgtcga cgacgccgat cgccggcgac aggcccagac cggcggccac ctcgcggatt
     1081 cgctcggcca ggccggtcgg gttgaggccg ccggcgttgg acacgatgcg caccccgcgc
     1141 tcgagcgcgt cgcccaggca ctgctcgatc tgggtgacga acgtgcgggc gtagccgagc
     1201 gacgggtcgc gcagctggtc cttgcccagg atcagcatgg tgagctcggc caggtagtcg
     1261 ccggtgatca cgtcgaccgg tccgccgtcg agcatctcgc gcatggccga caggcggtcg
     1321 ccgtagaacc ccgagcagtt ggcgacgatc aacggttcgg tcgccgagcc ggccgcgccg
     1381 cgcgcggtgt cgcccatcac gtcgctcctc ttgtcggtgt gcgtcccgag ccgggcggcc
     1441 cggcgaacgc ctgggcgatg tcgagccacc ggtccgcgtc cgcgccggtg gcgaccaggt
     1501 ccaggtcgtc gcggtggcgg cggtgggtga ccagcaggca gaagtcgtag gccggaccga
     1561 tcaccgactg cacggcgccg tcgggacccc acgtccaggt ctcgcccgac ggggcgcgca
     1621 gctcgacgcg gaaggcctcg gccgggggtt ccagcgagtg ggtggcgtag gcgaagtcgc
     1681 gggtgcgcac ccccaggtgg gcgacgtggc ggatgcggtc ggtgggctcg gcggtgacgc
     1741 ccagggcatc cgcgacgtcc aggccgtggg cccaggtctc catgaaccgc gccgtcgcca
     1801 tcgacgccgg cgacatcggc ggcccgaacc acggaacctt ctcgccctcc gggtgggcgc
     1861 gcagcacctc gggcaggcga cgtcgggcgg tgcgccaccg atcgagcaga tcggcggcgg
     1921 gggcggtggc cgctgcggcc gcggtggcgt cgacgaaccc ctcggggtcg cccatcgcct
     1981 ccaggatcag ctgctcccag ccggtggggt cggtggcggc gaccacgacc acctcgtcgg
     2041 tccaggccag gtgggcgatg gtcgtggcga cgtcccagcc ggccgccggc gtcggggtcc
     2101 gccactgctc gtcgctcagc ggcgtgaccc agctctcgag ctggtctcct tcggcggtca
     2161 ggtcgtcgag gacgtcgtcg agaacgctca tggctgctcc ggttcggtga tcgggccgag
     2221 ctcgcggtcg aggacgccgg cccagcggtc cagcacgcgg ttgcgtcgcc gtgtgtcgtc
     2281 gctcagcgcc ttggccagcc ccaacccgcg gatcatgtcg agggtggact cgatcagctc
     2341 gcgagtgccc gggaccgact cgtcggcgtc cagcgcggcg accgccagct ggtgggtctg
     2401 acgtccgatc cggcgctcga ggggagcgac ggcggcgagc agctccgggt cggtgcgtgc
     2461 ggcgacccac agctccagcg cggcatcgaa caccggggag gtgacgtggt cggccagcat
     2521 gcgaagcacc ttgcggcggt cgccgggagc tacccgccgg gccgcgtcga ccaggtcgtc
     2581 gccgcgggcc tgggcgacgt gctccaccgc ggccaccagc aggtcggccc gggaggggaa
     2641 gtggtgcagc tgcgcgccgc gggagacccc ggcacgctcg gacaccagag tggtcgatgt
     2701 gccggagtag ccgcactcga tgaggcattc cacggcggcg tcgagcagcc gttcgcgcat
     2761 gacccggctg cgctcctcct ggggcacccg ggtcacggtc acccgtcgat cttccgatgg
     2821 atccaacaaa cagtcaagac tgattgtttg tttggtcggc tgctcggccc ccgacgtcgg
     2881 acgctcagag gcggagggcg gcgaagatca ccatcgccac ggcgacgacc gtggccacgt
     2941 tcgtcccgat cgtcaggccg tacatggacc ggaaccgctc gtccatgcgg tcgtcgatcc
     3001 gcaactccat cgccgccatc tcggtgcgga gcacggccat gtcgtccttg agctcggtgt
     3061 ggagcagggc cgtctcgtct ttgagttcgg tgcggaggac ggccgtctcg tccttgagct
     3121 cggttcggag gaccgccatc tcgtctttga gttcggtgcg gagcagggcc gtctcgtcct
     3181 tgagctcggt tcggaggacc gccatctcgt ctttgagctc ggttcggagg acggccgtct
     3241 cgtccttgag ctcggttcgg aggaccgcca tctcgtcctt gagctcggtt cggaggacgg
     3301 ccgtctcgtc tttgaactcg atgcggagga ccgccatctc gtccttgagc tcggttcgga
     3361 gcacggtcat gtcgtccttg agctcggtgc ggagcagggc cgtctcggcg cggaggaact
     3421 ccttggtggc gggttcctcg acgtcgcgtg ccgggaagta cgagagcatc tcctccacgg
     3481 cctcctcgtc gatgacggtg gtcagcccct ggtacagggc ggatcggttt cgttcggtca
     3541 gggccatctg gcttcctcgg tccgggcgcc cgacgtgggt cggcgctctc gacgggggaa
     3601 gtcggtgacg ctgtcgtcga ggtcagggta gggcgatgca ctgacaccgg caacgggtcc
     3661 ggtgccggca gcccaccatc cgaggtctct cagccgaccc cgctgcacga gaccgggggt
     3721 tccttgctgc gcagtcgcag ggcggtctcg accgcctcga tcagggtcgc cgcggtcacc
     3781 ggcgtctcga cgatgtggac ccggtcccgg tcgaaccacc agctgtcgga ctcggtgcgg
     3841 cgttccagtt ccggggtcgt catctcggtg atcacggccg gtggccggcg ctcgtcggtg
     3901 atcgccccca gcacctcccg tctgacggga tcggtcgacc cgaagaggtt caccaccaca
     3961 tcggcgcccg acaccaggtt gcacgctccg gtcgcgacca gcgggcaggc gtggtgcggt
     4021 acgggtccct cgcagacacg gacctcgtag ccctcccggc gcaggacctc ggcgacgagg
     4081 tcgggggtgt tctccggcgg gcactcgacg agaacccgca cgcggccgcg gggagcgtcg
     4141 cgccaggtgt cggtggagag tggtcggccc atgggcacct ccgtgcgggg gtcgtcccca
     4201 tccacgatgg gcggcccggt cgcgttcggc aacgacccgc ccggtgattg gcgtcacacc
     4261 ccgctttgtc ccgcacaggc gcggtgatca ccgcgcctgt gcgggacaaa gcagggtggg
     4321 gtcagccgaa ctgggaacgg gtcgtggcga ggatcgcccg agcctcggcg acgacggcga
     4381 ccgcctggat ccgctgctcc gggaacggca gggcgatcgc ccggccccac gccgtctgga
     4441 actcggcgag caccgtctcg aggtggccca acgaggaccg agcccgacgg acctcggcgc
     4501 ccagcgtcgt cgggtcgcgg gtgaccctcg acgcggcctg ggccaggtcg accgccagtc
     4561 gctcctcggg gtgcagctca tagccggcgg acacctcgcc gacccgctcg gccaccgcat
     4621 cgaggcggcg ggccagaccc gggtacccgt cgagcatccg atcgatcgaa cggggccggt
     4681 cccggtcccg caccaaccgc accgtcgtcg tggcccgcag gtcgatccgc ggcatccgca
     4741 ccacgaccga ggccgcggcg gtgtcgtggt accagtagcc ccggccggcc cggatcccgg
     4801 tgccgtccgg agcagtggcc atcggcaacg cgcgaccgtc gacctcgacc gaccgtggga
     4861 ccgcctcggc cacgatgcgc acctcgatcg gccggtgccg ctcccagccg gcgtagtcgc
     4921 cacgggccgg accgatccgc accgagcggg cccggtcggt gacccgttgg cgcagccgca
     4981 ccgtcaccga gtcgccccgc aggtagtcgg gcgagacccc gtcgtcctcg tagagctcgc
     5041 cgtcgccgtc gccgccggga taggcggtga tgaccgggtg cggatagcag ggggcgtcga
     5101 gccgctgggc gtcgcgctgg ccggcgatca ccgcccccgc cgcggcgaac accggcacct
     5161 cgtcgagcag gtagcggcgc tcgaaccact cgccgtcggc ccgccggacc gtgaccgtct
     5221 cgccgagtgc cgtgtcgtac caaccacccc gggggagcca cacccggatc ggcgccatcc
     5281 ggtcgtcgtc gagaggtgtg accaccggcg ccaccaccag gtcgtcgccg aggaggtact
     5341 ggccgtcggc gacgtaggcg gcgtcccggt cggggtggtc gtggtacatc ggacgaagcg
     5401 ccgacacgcc ggtgtcgacc gcccgtcggc actgcccgta caggtacggg accagctcgt
     5461 agcggcggtg cacggctcgg atcatcaccg accggtgcgg atcggcgaac tcccagaagc
     5521 gacggtcctg ggccgggtcc ttggtggcgt gggtgcgcaa cacgggcgag aacgccccga
     5581 actgcatcca gcgggtgtac agctcgggac tgggcggctc gccggtgtga ccaccgatgt
     5641 cgtgactcca gaagccgtac ccgacgttcg cggcggtcgc ggtgaaccac ggctgcaggg
     5701 cgagcgagtc ccaggtcgac cacgtgtcac cggagaagcc gatcgggtag cggccgccac
     5761 ccatcccgcc ccaccggctg aacacgagcg gccgccggtc gggccggcgg gactgctggt
     5821 cgttccagtg cagctcgttc agccacggca gggggtcgag cccctccacc gccgaggtgg
     5881 tgccctgctg ccagtccatc caccagaagt cgactccccg gtcctcctcg ggatggtgca
     5941 ggaaccggaa gtacgcctcg atgaacccgg ggtcggtcac cgcgaacggg atccgctcac
     6001 cggaggcggg atcgaccccg accgcccgtg ccacctcggg gtagcggtcc tcgtgcggcc
     6061 cgaccccatc ggccgggtgc acgttcaacg tgacccgcag gcctctccga tgcagccgct
     6121 ccagggtgtc ggtcgggtcg gggaagtagc gccggtccca cgagtagccg gtccaacccg
     6181 ggaggtgcca gtccatgtcg atgaccatca cgtcgacggg cacgtccatc gtgtcgaacc
     6241 gctcggcgat gtcgagcagg tcccggtcgg tgtatgccca gtagcggctg taccagtagt
     6301 cgaaggcgta gcggggcggc agcggctgcg gtccgatcag cgcggcggcg tcggccagcg
     6361 cgccgcggtg g
//
LOCUS       DAOQOD010000177         6114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5026_length_6114_cov_11.736260, whole genome shotgun sequence.
ACCESSION   DAOQOD010000177 DAOQOD010000000
VERSION     DAOQOD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_6114_cov_11.736260"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..246)
                     /locus_tag="PLZ72_13545"
     CDS             complement(64..246)
                     /locus_tag="PLZ72_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37369.1"
                     /translation="MSDTTDTGSEDSAGGGSHWLRWMVLIAIAIGAIVAGRKIALNSA
                     DKDFEERLRELDAERG"
     gene            286..368
                     /locus_tag="PLZ72_13550"
     tRNA            286..368
                     /locus_tag="PLZ72_13550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Leu,seq:caa)
     gene            433..1230
                     /locus_tag="PLZ72_13555"
     CDS             433..1230
                     /locus_tag="PLZ72_13555"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNA37370.1"
                     /translation="MAAPSSGRRRRRGRRSARVPDRDDASRRARRRMVAHDLAARDIH
                     DERVLAAMESVPREHFVPEDLVDAAYEDRPLPIGAGQTISQPYIVALMAQEARIGAAD
                     RVLEVGTGSGYGAAVLGRLAAEVWTVERHEDLARRAREALEATGAGNVHVDVGDGSLG
                     APDSAPFDAIVVTAAAPAVPDALVAQLADGGRLVIPIGPDGHAQELVRVTVRGEHSER
                     AHLCDVRFVPLIGAEGFGRIHPGQQRRDGDESGPDGPRTDPGTPPEP"
     gene            1295..1849
                     /locus_tag="PLZ72_13560"
     CDS             1295..1849
                     /locus_tag="PLZ72_13560"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HNA37371.1"
                     /translation="MANEWGPLATLAGEWQGEGGLDTAYSHSQEKVLDTPYLEKATFK
                     PFGPVDNGSQSLYGLDYHSAMWRGNEDIPFHTEVGYWLWDAATGEVMRCFVVPRGITT
                     FAGGTAAADATEFTLSASAGHDTYSIGENKYLAQHASTRAYEVTITIDGPDTWSYHET
                     TTLRMDQFPELFPHTDHNTLHRVA"
     gene            complement(1924..5133)
                     /locus_tag="PLZ72_13565"
     CDS             complement(1924..5133)
                     /locus_tag="PLZ72_13565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNA37372.1"
                     /translation="MAVELHRPVHPVRFITAASLFDGHDAAINIMRRILQSQGAEVIH
                     LGHNRSVDEVVEAAVAEDVQGVAVSCYQGGHVEFFGYLVDRLAAAGAGHIRVYGGGGG
                     VIVAEEIAALRSLGVRIFSPEDGQRMGLAGMVNTLIAECDVDLTDQPAPIEDLLGGSV
                     PALARTITMLEADRLDASTVEQIAAAARARTVPVLGITGTGGSGKSSLTDELIRRVRL
                     DQDDKVNIAVLAVDPTRRRGGGALLGDRIRMNAIHTPAVYFRSLATRDAGGEVPLHLD
                     RMVQATVAAGADLVIVETPGIGQGDSEITTISDVALYVMTPEFGAASQLEKIDMLDFA
                     DAVAINKFERRGAQDALRDVARQMVRNREAFDRDPADMPVFGTIASRFNDDGVTALYQ
                     HLRRELAGHGLGVGEGSLAPVDTRSSTSLDPIVPESRARYLAEIAESVRSYHDATARH
                     AATARRLQQLEATAAILADSRGDGGGGVPDAVTALAEATRAELPDADRELLERWPERV
                     AELTGPDRPTRESLAGTAVPKLALPRFVDHGEQLRWLRSENLPGSFPFTAGVFPFKRE
                     GEDPARMFAGEGGPARTNRRFHLLSEGQPATRLSTAFDSVTLYGCDPAERPDIYGKVG
                     NSGVSVATLDDMRVLYSGFDLCDPTTSVSMTINGPAPTVLAMFLNTVIDQRIDRFTEE
                     QGRPPTDDEAAELRAGALAHVRGTVQADILKEDQGQNTCIFSTEFSLGLMADVAEWFV
                     AEGVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTYVESYLARGLDIDDFAPNLSF
                     FFSNGMDPEYTVLGRVARRIWAIAMRDRYGAGERSQKLKYHVQTSGRSLHAQEMAFND
                     IRTTLQALCATYDNANSLHTNAYDEAVTTPTAESVRRALAIQLVINREWGLAMNENPL
                     QGSFIVEELTDLVEAAVLAELDAISSRGGVLGAMETGYQRGRIQDESVLYEHRKHDGS
                     LPIVGVNTFLDPAGEPEPPVLTLARSTDAEKDDQIARVCDFQARHAAERPAALERLRR
                     AALDGENLFAVLMDTVRSCTLGEITDALFEVGGQYRRSV"
     gene            5223..5732
                     /locus_tag="PLZ72_13570"
     CDS             5223..5732
                     /locus_tag="PLZ72_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNA37373.1"
                     /translation="MRQRRTLGFDPIDEARRQWIDRGWQDAAGGMAALTSIIRVEQLL
                     MARVEEALAPFELTFARYELLVLLDFSRAGALPMGKLGARLQVHPASVTNAVDRLEAQ
                     ALVERVAHPDDGRTTLATITSTGRRRVRRATAVLNEAVFSDLGWSERDLRDLFRILRK
                     LRRGAGDFD"
     gene            complement(5767..>6114)
                     /locus_tag="PLZ72_13575"
     CDS             complement(5767..>6114)
                     /locus_tag="PLZ72_13575"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNA37374.1"
                     /translation="LRSRLARLAAIGGWDGPAPTFTSPTAPDPDGSTVELAALVGAAY
                     RALADAGSDLVVASLEDAVGQTHRPNLPGTVDEHPNWSLALPVLIEDLDRTDPRNWLR
                     YGLPPRTERRPQL"
BASE COUNT          846 a         2082 c         2289 g          897 t
ORIGIN      
        1 gctggcgttg ttgtagctgg caggtcgccg gacgacccgt ggcctacaag ttcggcggcg
       61 gggtcagccc cgctcggcgt cgagctcgcg caggcgctcc tcgaagtcct tgtcggcgga
      121 gttcagggcg atcttgcggc cggcgacgat cgcgccgatc gcgatggcga tcagcaccat
      181 ccagcgcagc cagtgcgagc cgccgcccgc ggagtcctcg gaaccggtgt cagtcgtgtc
      241 gctcatgggc cacacgctac ggcatcccgc tagagtccgg ggccggcccg ggtggcggaa
      301 tggcagacgc ggcggactca aaatccgctg tccgaaaggg cgtgtgggtt cgagtcccac
      361 cccgggcacg ccgagaaccg agcgatcggg agccaccacc cgccacgtgg ccgggcctag
      421 cctgatgtcg tggtggccgc accgtcgtcc ggtcgacgtc gccgccgtgg tcgacgttcg
      481 gcacgggtcc cggaccggga cgacgcgagc cgacgggccc gccgtcgcat ggtcgcccac
      541 gacctggctg cccgggacat ccacgacgaa cgggtgctcg cggccatgga gtccgtgccg
      601 cgggagcact tcgtccccga ggatctggtc gacgcggcct acgaggatcg tcccctgccc
      661 atcggtgcgg gccagacgat ctcccagccc tacatcgtcg ccctgatggc acaggaggcc
      721 cggatcggag cggccgaccg ggttctcgag gtggggaccg gttcgggcta cggcgccgcg
      781 gtgctcggcc ggctggccgc cgaggtgtgg accgtcgaac gccacgagga cctcgcccgc
      841 agagcgcggg aggcgctcga ggcgacgggg gcgggcaacg tgcacgtcga cgtgggcgac
      901 ggctcgctcg gggctccgga ctcggcgccc ttcgacgcga tcgtcgtcac cgcagccgct
      961 ccagcggtcc ccgatgccct ggtcgcccag ctcgccgacg gtggccgcct cgtcatcccg
     1021 atcggtcccg acggccacgc ccaggagctg gtgcgggtca cggttcgggg ggagcacagc
     1081 gagcgagccc acctgtgcga cgtccgcttc gtccccctca tcggggccga ggggttcgga
     1141 cggatccacc ccggacagca gcgacgggac ggtgacgaga gcggacctga cggaccgcgg
     1201 acagatccgg ggacaccgcc tgagccgtga tgggtacgat ccgtcctgcg ccgacccgga
     1261 ccctcgggtc gacccgagac gaggagcttg gaccatggcg aacgaatggg gacccctggc
     1321 gacgctggcc ggcgagtggc agggcgaggg tggtctcgac accgcctact cgcactcgca
     1381 ggagaaggtg ctcgacacgc cgtacctgga gaaggcgacg ttcaagccgt tcggtccggt
     1441 cgacaacggc agccagtcgc tctacggcct cgactaccac agcgccatgt ggcgcgggaa
     1501 cgaggacatc ccgttccaca ccgaggtcgg ctactggctg tgggacgccg ccaccggtga
     1561 ggtcatgcgc tgcttcgtcg tgccgcgggg catcaccacg ttcgccggcg gcaccgcggc
     1621 tgcggacgcg acggagttca cgctgtcggc ctcggccggc cacgacacct acagcatcgg
     1681 cgagaacaag tacctggccc agcacgcctc gacgcgggcc tacgaggtca cgatcaccat
     1741 cgacggcccc gacacgtggt cctaccacga gaccaccacg ctgcggatgg accagttccc
     1801 cgagctgttc ccccacaccg accacaacac gctgcaccgc gtcgcctgag aacccgcgtc
     1861 gcctgagaac ccgggtcgcc cgagatcccg cgtcgcctga cccgcgggat cgtcgccggt
     1921 ggctcagacg ctgcggcggt actgaccgcc cacctcgaac agcgcgtcgg tgatctcgcc
     1981 gagcgtgcag gaccgcaccg tgtccatcag gaccgcgaac aggttctcgc cgtcgagcgc
     2041 cgcccgtcgc agccgctcga gcgcggcggg tcgctcggcg gcgtggcggg cctggaagtc
     2101 gcacacgcgg gcgatctggt cgtccttctc cgcgtcggtc gagcgggcca gggtcagcac
     2161 cggcggttcg ggttcgccgg ccgggtcgag gaacgtgttg actccgacga tcggcaggct
     2221 cccgtcatgc ttgcggtgct cgtagaggac cgactcgtcc tggatgcggc cccgctggta
     2281 gccggtctcc atcgctccga gcacgccgcc gcgtgagctg atggcgtcga gctcggccag
     2341 caccgccgct tcgaccaggt cggtgagctc ctcgacgatg aagctgcctt gcaacgggtt
     2401 ctcgttcatg gccaggcccc actcgcggtt gatgacgagc tggatcgcca gcgcccgacg
     2461 caccgactcg gcggtggggg tcgtgaccgc ctcgtcgtag gcgttggtgt ggaggctgtt
     2521 ggcgttgtcg taggtggcgc acagcgcctg cagcgtggtg cggatgtcgt tgaacgccat
     2581 ctcctgggcg tgcagggacc ggcccgaggt ctgcacgtgg tacttgagct tctgggagcg
     2641 ctcgccggcg ccgtagcggt cccgcatggc gatcgcccag atgcgtcggg cgacccggcc
     2701 gagcaccgtg tactcggggt ccatgccgtt ggagaagaag aagctgaggt tgggggcgaa
     2761 gtcgtcgatg tcgaggccgc gggccaggta cgactcgacg taggtgaacc cgttggccag
     2821 ggtgaacgcc agctggctga tcgggttcgc gcccgcctcg gcgatgtggt agccggagat
     2881 ggacaccgag tagaagttgc gcaccccctc ggcgacgaac cactcggcca cgtccgccat
     2941 gagtccgagc gagaactcgg tggagaagat gcaggtgttc tggccctggt cctccttgag
     3001 gatgtcggcc tgcaccgtgc ctcggacgtg ggcgagcgcc ccggcgcgca gctcggcggc
     3061 ttcgtcgtcg gtgggcggcc ggccctgctc ctcggtgaag cggtcgatgc gctggtcgat
     3121 gacggtgttg aggaacatgg cgagcacggt cggagccggg ccgttgatgg tcatcgacac
     3181 cgacgtcgtc gggtcgcaca ggtcgaagcc cgagtagagg acccgcatgt cgtcgagggt
     3241 cgcgaccgac acgccggagt tgccgacctt gccgtagatg tcgggccgct cggccgggtc
     3301 gcagccgtag agggtgaccg agtcgaacgc ggtcgacagt cgggtggcgg gttggccttc
     3361 cgacagcagg tggaagcggc ggttggtgcg cgccgggccg ccctcgccgg cgaacatgcg
     3421 ggccgggtcc tcgccctcgc gcttgaacgg gaacaccccg gcggtgaacg ggaacgagcc
     3481 cggcaggttc tccgagcgca gccaccggag ctgctcgccg tggtcgacga agcgggggag
     3541 cgccagcttg gggactgcgg tgcccgccag cgattcgcgg gtcgggcggt cgggtccggt
     3601 cagctcggcc acgcgctcgg gccaccgttc gagcagctcg cggtccgcgt cggggagctc
     3661 ggcgcgggtg gcctcggcga gggcggtcac cgcgtcggga actccaccgc ccccgtcgcc
     3721 gcggctgtcg gcgaggatgg ctgcggtggc ctcgagctgc tggagccggc gggccgtcgc
     3781 cgcgtgacgg gcggtggcgt cgtggtagct gcggacggac tcggcgatct cggccaggta
     3841 gcgcgcacgg gactcgggga cgatcgggtc caggctcgtc gacgaacggg tgtcgaccgg
     3901 ggccagggat ccctcgccga cgcccaagcc gtggccggcg agctcccggc gcaggtgctg
     3961 gtacagggcg gtgacgccgt cgtcgttgaa ccgggacgcg atcgtgccga acaccggcat
     4021 gtcggccggg tcccggtcga acgcctcgcg gttgcggacc atctggcggg cgacgtcgcg
     4081 gagggcgtcc tgggcgccgc gacgttcgaa cttgttgatc gccaccgcgt cggcgaagtc
     4141 gagcatgtcg atcttctcca gctgcgacgc ggcgccgaac tcgggggtca tcacgtacag
     4201 cgccacgtcg gagatggtgg tgatctccga gtcgccctgg ccgatgccgg gggtctcgac
     4261 gatgaccagg tcggcgccgg cggccacggt ggcctggacc atccggtcca ggtgcagggg
     4321 tacctctccg ccggcgtcgc gggtggcgag ggaccggaag tacaccgcgg gggtgtggat
     4381 ggcgttcatg cggatccggt cgccgagcag cgccccgccg ccgcggcgcc gggtcgggtc
     4441 gacggcgagc accgcgatgt tgaccttgtc gtcctggtcg aggcggaccc gtcggatgag
     4501 ctcgtcggtc agcgacgact tgcccgagcc gccggtgccg gtgattccga gcaccggcac
     4561 cgtccgcgcc cgagccgcgg cggcgatctg ctcgacggtc gacgcgtcga ggcggtcggc
     4621 ctcgagcatg gtgatcgtgc gggccagtgc cgggacggac ccgccgagca ggtcctcgat
     4681 cggggcgggc tggtcggtca ggtcgacgtc gcactcggcg atcagggtgt tgaccatgcc
     4741 ggccaggccc atgcgttggc cgtcctcggg ggagaagatg cgcacgccga gggaccgcag
     4801 cgccgcgatc tcctcggcga cgatgacgcc gccgccgccc ccgtagaccc ggatgtgtcc
     4861 cgcgcccgcg gcggcgagcc ggtcgaccag gtagccgaag aactcgacgt ggccgccctg
     4921 gtagcagctg accgcgacgc cctggacgtc ttcggcgacg gcggcctcga ccacctcgtc
     4981 gaccgagcgg ttgtggccca ggtggatcac ctctgcgccc tgcgactgca ggatgcgccg
     5041 catgatgttg atcgcggcgt cgtggccgtc gaacaggctc gcggcggtga tgaagcggac
     5101 cgggtgaacc ggtcggtgga gctcgacggc cacgtgtggt ccccccggca gatgtttgga
     5161 cgtccacata ttagcggcga cggcctccgc gtcgagcggc gacgggcgat agcgtcccgg
     5221 agatgcggca gcggcggacc ctcggtttcg acccgatcga cgaggccagg cgccagtgga
     5281 tcgatagggg gtggcaggac gcggcgggcg gaatggccgc gctgacgtcc atcatccggg
     5341 tcgagcagct gctcatggcc cgggtcgagg aggcgctcgc tccgttcgag ttgaccttcg
     5401 cccgctacga gctgctcgtc ctgctcgact tctcccgggc cggcgcgctg ccgatgggca
     5461 agctgggcgc ccgcctgcag gtgcaccccg ccagcgtcac caacgcggtc gaccgcctcg
     5521 aggcgcaggc actggtcgag cgggtcgcgc accccgacga cggccgtacc accctggcca
     5581 cgatcacctc gaccgggcgg cgccgggtcc gccgtgccac cgcggtgttg aacgaggcgg
     5641 tgttcagcga cctgggctgg agcgagcgcg acctccgcga cctgttccgg atcctgcgca
     5701 agctgcgccg cggtgcgggg gacttcgact gaacccgaac tgccggtcgg gtttccggcg
     5761 tgttggtcag agctgtggcc gccgctcagt ccgtgggggc agcccgtacc ggagccagtt
     5821 ccgagggtcg gtgcggtcga gatcttcgat gaggaccgga agcgccaggc tccagttcgg
     5881 gtgctcatcg acggtgccgg gcaggttggg tcggtgggtc tggccgacgg cgtcctcgag
     5941 cgaggccacc accaggtcgc tgcccgcgtc ggccagcgcc cggtacgcgg ctccgaccaa
     6001 cgcggccaac tccaccgtcg atccgtccgg gtccggtgcc gtcgggctcg tgaacgtcgg
     6061 cgcgggcccg tcccagccgc cgatggcggc caggcgggcc aggcggctgc ggag
//
LOCUS       DAOQOD010000178         6096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5050_length_6096_cov_9.868068, whole genome shotgun sequence.
ACCESSION   DAOQOD010000178 DAOQOD010000000
VERSION     DAOQOD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_6096_cov_9.868068"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1025
                     /locus_tag="PLZ72_13580"
     CDS             108..1025
                     /locus_tag="PLZ72_13580"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNA37375.1"
                     /translation="MHDERALELATARHGVVAMWELRRDGVPAGDIRWLQQSRHWELV
                     APGVLRRVGSHRSTAQALAVGVLGCGPSTAAATLGAARWWGLTGCAQWPLDVVGTRTL
                     RHVPDRVRYRQIRRLPGTWTTTLDGVPVVRPELLALQLFGACRPQRASRLVDSLWSMR
                     VLSGPSIATFLGDMGAMGRNGTAGLREYLAERGPDYEPPASRLEGRAWEILAEAGIEV
                     RRQVNLGSEAWTGRVDGLVVGTRVVIEIQSWRYHGALVDREADALRIKHLEADGFTVV
                     EVTDDHVWSDPGEVIRRVSAAIVLDRTRR"
     assembly_gap    1089..1188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..2399)
                     /locus_tag="PLZ72_13585"
     CDS             complement(<1189..2399)
                     /locus_tag="PLZ72_13585"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNA37376.1"
                     /translation="MAKIKVQNPVVELDGDEMTRIIWEFIKDKLILPYLDVDLKYYDL
                     SIESRDATDDQITVDAANAIKQYGVGVKCATITPDEARVEEFGLKHMWRSPNGTIRNI
                     LGGVVFREPIICSNIPRLVPGWTKPIVIGRHAHGDQYRATDFKAPTAGTVTITYTPAD
                     GSAPIEHEIVQIPDGGGVVMGMYNFDDSIRDFARASFRYGLQRNYPVYLSTKNTILKA
                     YDGQFKDLFQEVFDAEFADEFAAAGLTYEHRLIDDMVAAALKWDGGYVWACKNYDGDV
                     QSDIVAQGFGSLGLMTSVLMTPDGRTVEAEAAHGTVTRHFREHQKGNPTSTNPIASIF
                     AWTRGLAHRGKLDNTPEVTAFADSLEQVCVSTVEGGQMTKDLALLVGKDQEWLTTEQF
                     LAALDENLQKHL"
     gene            complement(2558..3493)
                     /locus_tag="PLZ72_13590"
     CDS             complement(2558..3493)
                     /locus_tag="PLZ72_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37377.1"
                     /translation="MYDDVALALAEHRHGVVATYELAEHGMSPHHIEQLLSSRHWERV
                     SRSVLRRRGSTRTDDQALASVVLDYGPNAYVSALSAGRLFGLSGCPMRPVSVVGTVAT
                     RRKRADVGYHRVRRVAPRWTTEVRGIPVVAPELCAMQLFACTRYESAERRVDSMWSTG
                     RLSGRSVEVFLADMGRSGRNGIAGLRRYFEDRGVRYRPPDSGVEGRAIQVLRDTGIEL
                     RRQVDVGSVEAWTGRVDLMVVGHPVVVEIQSDTFHASLVNRERDRVRRADLEAAGFQW
                     VELWETDVWARPWTLAPAVRVGIDRARRHRTPPVL"
     gene            complement(3647..4759)
                     /locus_tag="PLZ72_13595"
     CDS             complement(3647..4759)
                     /locus_tag="PLZ72_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37378.1"
                     /translation="MSERVGTSGRRGDRPGRSHLRGRPVAVIAATLIAAVAILGATAC
                     GGDSGAKGTTTTSKKGTSTIADTGEGDTPAALMTAVCEGAPKITDAGEVDTPLVNEAS
                     GLVASWSNTGDGADGVWWIHNDSGSAPAIFAINGRGQLLATVALEGAEARDWEDIAVG
                     PPAVAGGPSQLYVGDIGNNKAMLGDATARGSVRVYRLDEPVVPTEPPAAGSVAPVVTA
                     NVTTFSLRYPDKPYDAEAMVVDPVRGDIWIITKDWERAGKSLLFRMPKAAEIAEGTSI
                     DMLPAGEVPLEPGTLVTSADVTRDGSLVALRSYGAVDLYRRPSGKKLSAAFGTTPCGG
                     PVPNEVQGESVAFAPDGGSYLTVSEGDKPMLHRTSP"
     gene            complement(4756..5655)
                     /locus_tag="PLZ72_13600"
     CDS             complement(4756..5655)
                     /locus_tag="PLZ72_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNA37379.1"
                     /translation="MEIGVALPTMARDYTRRTTVTWSQGIDAGPFSSVSCGERISFHN
                     QEMIVTNSAAAALTERVRVFVNIAVGPLHSAGMLAKQMATLDVLGEGRLTVGLGVGGR
                     AHDYRSAGSSFERRHQRLDDLVAELKRLWAGHPPFDGADPIGPAPVQHGGPELLSAAM
                     GRKALARAARWADGNSGFSIDADGAGLAAAVTAAERAWTDAGRTDAPRHVTGCFCVLG
                     TGDDQGVLQAFTYDYLAVFGDRFARAMADAAAVFSAERLNRVLDDAEAAGVDEFILVP
                     GTTDPACLSALTATVRERIGSTS"
     gene            complement(5778..>6096)
                     /locus_tag="PLZ72_13605"
     CDS             complement(5778..>6096)
                     /locus_tag="PLZ72_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37380.1"
                     /translation="GFAGLAVILLFVGAVVVLAGPLARRLHAWRERRALRRAANRLAA
                     ERWDVRVERELEELGREVGRPRAPHETVSAHARDLATVTGRPELAEQGRAVDDHRYRP
                     PPL"
BASE COUNT          855 a         2083 c         2145 g          913 t
ORIGIN      
        1 cgctttgtcc cgcacaggcg cggtgatcac cgcgcctgtg cgggacaaag cgggaccggg
       61 tcgcggtccc cggtgacacg ggcgtcgatg ggcgtcacgg tggtggggtg cacgacgaac
      121 gagcgctcga gctggccacc gcacgccacg gcgtcgtcgc gatgtgggag ctgcgccgtg
      181 acggcgtccc cgccggcgac atccgctggc tgcagcagtc acggcactgg gagctcgtgg
      241 ctcccggggt gctgcgacgg gtgggcagcc accgctcgac cgcccaggcg ctcgccgtcg
      301 gggtcctggg ctgcgggccg tcgaccgccg ccgcgaccct gggcgcggcc cgctggtggg
      361 ggctgaccgg ctgtgcgcag tggccgctcg acgtggtggg cacccgaacg ctgcgacacg
      421 tccccgatcg tgtgcgctac cgccagatcc gtcgcttgcc gggaacgtgg accacgaccc
      481 tcgacggcgt gcccgtcgtc cggcccgagc tgctggcgct gcagctgttc ggcgcgtgtc
      541 gaccccaacg ggcgagccgg ctggtcgact cgctgtggtc catgcgggtg ctgtcgggcc
      601 cgtcgatcgc gaccttcctc ggcgacatgg gagcgatggg ccgcaacggc accgccggcc
      661 tacgggagta cctggccgag cgcggcccgg actacgagcc gccggcgtcc cggttggagg
      721 gtcgggcgtg ggagatcctg gccgaggcgg gcatcgaggt ccgccgccag gtgaacctgg
      781 gatccgaggc gtggacggga cgggtcgacg gactcgtcgt cggcacccgc gtcgtgatcg
      841 agatccagag ctggcgctac cacggcgcgc tggtcgaccg cgaggcggac gctctgcgga
      901 tcaagcacct cgaggccgac ggcttcaccg tcgtcgaggt caccgacgac cacgtgtggt
      961 ccgatccggg cgaggtcatc cggcgggtga gcgccgcgat cgtgctcgat cgaacccgtc
     1021 gttgacccca aacccccagc tttgtcccgc acaggcgcgg tgatcaccgc gtctgtgcgg
     1081 gacaaagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gtgcttctgc
     1201 aggttctcgt ccagcgcggc caggaactgc tcggtggtca gccactcctg gtccttgccc
     1261 accagcagcg ccaggtcctt ggtcatctgg ccgccctcga cggtcgacac gcacacctgc
     1321 tcgagcgagt cggcgaacgc ggtgacctcg ggcgtgttgt cgagcttgcc ccggtgcgcc
     1381 aggccacgcg tccaggcgaa gatcgacgcg atcgggttgg tcgaggtcgg gttgcccttc
     1441 tggtgctccc ggaagtggcg ggtcaccgtg ccgtgcgcgg cctcggcctc gacggtgcgg
     1501 ccgtcgggcg tcatcagcac cgacgtcatc aggccgagcg agccgaagcc ctgggcgacg
     1561 atgtcggact gcacgtcgcc gtcgtagttc ttgcacgccc acacgtatcc gccgtcccac
     1621 ttgagcgcgg cggcgaccat gtcgtcgatc agacgatgct cgtaggtgag accggcggcg
     1681 gcgaactcgt cggcgaactc ggcgtcgaag acctcctgga acaggtcctt gaactggccg
     1741 tcgtaggcct tgaggatcgt gttcttggtc gacaggtaca ccgggtagtt gcgctggagg
     1801 ccgtagcgga acgacgcccg ggcgaagtcg cggatcgagt cgtcgaagtt gtacatgccc
     1861 atgacgacgc cgccgccgtc ggggatctgc acgatctcgt gctcgatggg cgccgagccg
     1921 tcggcggggg tgtaggtgat ggtgacggtg ccggcggtcg gggccttgaa gtcggtggcg
     1981 cggtactggt cgccgtgggc gtgacggccg atgacgatcg gcttggtcca gccgggcacc
     2041 aggcggggga tgttcgagca gatgatcggc tcacggaaca cgacgccgcc gaggatgttg
     2101 cggatggtcc cgttgggcga gcgccacatg tgcttcaggc cgaactcctc gacgcgggcc
     2161 tcgtcggggg tgatggtggc gcacttgacg cccacgccgt actgcttgat ggcgttggcg
     2221 gcgtcgacgg tgatctggtc gtcggtggcg tcacgggact cgatcgacag gtcgtagtac
     2281 ttcaggtcga cgtcgaggta cggcaggatc agcttgtcct tgatgaactc ccagatgatg
     2341 cgggtcatct cgtcgccgtc gagctcgacg accgggttct ggaccttgat cttcgccatg
     2401 gctgtgcgtt cctctgagtt ctcgggaccg gcgcggcagg gccggtcgac gggcgcccga
     2461 aagcatacat gttgcagaca agtgcgctcc cgcagacaac actccccgcc ccaggggttc
     2521 tgtgatgcac cttcccggga tttcccggtg aggagcatca cagaacgggt ggggtccgat
     2581 gcctgcgggc gcggtcgatc ccgacacgaa ccgcaggagc gagggtccac ggccgggccc
     2641 acacgtcggt ctcccacagc tccacccact ggaacccggc agcctcaaga tccgcacggc
     2701 ggacccggtc ccgctcacgg ttcaccagtg aggcgtggaa ggtgtcggac tggatctcca
     2761 ccaccaccgg atggccgacc accatcaggt ccacccgacc cgtccacgcc tcgacggagc
     2821 cgacgtccac ctgtcgtctc agttcgatcc cggtgtcgcg gaggacctgg atcgcccggc
     2881 cctccacccc gctgtcgggc ggacggtatc ggacgccacg atcctcgaag taccggcgga
     2941 gcccggcgat cccgttgcga ccggagcgtc ccatgtcggc caggaacacc tcgaccgagc
     3001 gtcccgagag gcgtcccgtc gaccacatcg aatccacccg ccgctcggcc gactcgtagc
     3061 gggtgcaggc gaagagctgc atggcgcaca gctcgggagc caccaccggg attccccgca
     3121 cttcggtggt ccagcgcgga gcgacccgtc gcacccggtg gtagccgacg tcggcccgct
     3181 tgcgccgggt cgccaccgtc ccgacgaccg acacgggccg catgggacaa ccgctcagac
     3241 cgaagaggcg acccgcgctc agggcgctga cgtaggcatt cggtccgtaa tcgagcacca
     3301 ccgaggcaag ggcctggtcg tcggtgcgcg tggatccccg ccgacgaagg accgaccggc
     3361 tcacacgctc ccagtggcgc gagctcagca gctgttcgat gtgatgcggg ctcatcccgt
     3421 gctcggcgag ctcgtaggtg gcgaccacac cgtgtcggtg ctcggccagg gcgagggcga
     3481 cgtcgtcgta cacgtccccc acggtgagcc acgcacccag gcgttgtcag cggggaccgc
     3541 gacccggtcc tcggacccag ggctttgtga tgcttcttgc cgggctatcc cggggaagag
     3601 catcacagaa cgccgggggg ggttggccgg ggtttggaga agttggtcag ggagaggtgc
     3661 ggtgcagcat cggcttgtcg ccctcggaca ccgtcaggta cgacccgccg tcgggggcga
     3721 aggccaccga ctcgccctgc acctcgttcg gaaccgggcc accgcacggg gtggtcccga
     3781 acgccgcgga cagcttcttg cccgagggcc gccggtagag gtcgaccgcg ccgtacgagc
     3841 gcagcgcgac cagcgacccg tcgcgggtga cgtcggccga ggtgaccaac gtgcccggct
     3901 cgagcggaac ctcgccggcg ggaagcatgt cgatgctcgt cccctcggcg atctccgctg
     3961 ccttgggcat ccggaacagc agcgacttgc cggcccgctc ccagtccttg gtgatgatcc
     4021 agatgtcacc gcggaccggg tcgaccacca tcgcctcggc gtcgtagggc ttgtcggggt
     4081 agcgcagcga gaacgtcgtc acgttcgcgg tgaccaccgg ggcaaccgat cccgcggccg
     4141 gcggctcggt gggcacgaca ggttcgtcca gtcggtagac gcggacgctc ccccgagcgg
     4201 tcgcgtcgcc gagcatggcc ttgttgttgc cgatgtcgcc gacgtagagc tgcgacggtc
     4261 cgcccgccac cgcgggtggg ccgacggcga tgtcctccca gtcgcgggcc tcggcgccct
     4321 cgagcgcgac cgtcgccagc agctgacccc ggccgttgat ggcgaagatc gccggggcac
     4381 ttccgctgtc gttgtggatc caccagacgc catccgcgcc gtcaccggtg ttggaccacg
     4441 acgcgaccag acccgacgcc tcgttgacca gcggcgtgtc gacctcgccg gcgtcggtga
     4501 tcttgggtgc gccttcgcac acggcggtca tcagcgccgc gggcgtgtcg ccctcgcccg
     4561 tgtccgcgat ggtcgacgtt cccttcttcg acgtcgtcgt cgtgcccttg gcgcctgaat
     4621 caccgccgca cgccgtggcg ccgaggatcg cgaccgcggc gatcagcgtt gcggcgatga
     4681 ccgcgaccgg gcggccgcgc agatggctcc tgcccggccg gtcaccgcgc cgcccgctcg
     4741 tcccgacacg ttcgctcatg acgtcgatcc gatccgctcg cgcaccgtgg cggtcagcgc
     4801 cgacaggcac gccgggtcgg tggtgcccgg caccaggatg aactcgtcga ccccggcggc
     4861 ctcggcgtcg tcgagcaccc ggttcaggcg ttcggcgctg aacaccgcgg cggcatcggc
     4921 catggcccgg gcgaagcggt ctccgaacac ggccaggtag tcgtaggtga acgcctgcag
     4981 cacgccctgg tcgtcgccgg tgccgagcac gcagaagcag cccgtgacgt ggcggggagc
     5041 gtcggtgcga ccggcgtcgg tccaggcccg ctcggcggcg gtgaccgcgg cggccaaccc
     5101 ggcgccatcg gcgtcgatcg agaaccccga gttgccgtcg gcccagcgag cggcccgggc
     5161 gagcgccttc cttcccatgg ccgcggacag tagttcgggt ccaccgtgct gcaccggcgc
     5221 cggcccgatc ggatcggcac cgtcgaaggg cggatgtccg gcccacagcc gcttcagttc
     5281 ggcgacgagg tcgtcgagac gttggtggcg gcgctcgaag gacgaaccgg cggagcggta
     5341 gtcgtgcgcg cggccgccga cgcccaggcc gacggtgaga cgaccctcgc cgagcacgtc
     5401 gagggtggcc atctgcttgg ccagcatccc ggccgagtgc agcgggccga cggcgatgtt
     5461 gacgaacacc cggacccgtt cggtgagcgc ggctgctgcc gagttggtga cgatcatctc
     5521 ctggttgtgg aagctgatgc gctccccgca cgagacgctc gagaacggcc cggcgtctat
     5581 gccctgggac cacgtgacgg tggtgcgacg ggtgtagtcg cgggccatgg tgggcagggc
     5641 gacaccgatc tccatgtcga ccgacgctac cgaaccccca tcaccccggc gctgtaggtg
     5701 cacccctggg gttctgtgat gctgtttccc gggatctccc cggaaccagc atcacagaac
     5761 cctggtggtg cggggattca tagcggcggg gggcggtagc ggtggtcgtc gacggcgcgg
     5821 ccctgctcag cgagctcagg gcgacccgtc acggtcgcca ggtcccgggc gtgcgccgag
     5881 acggtctcgt gcggtgccct cggccgcccg acctcccgtc ccagctcctc gagctcgcgt
     5941 tcgacccgca cgtcccagcg ttcggcggcc agccggttcg cggcccggcg aagggcccga
     6001 cgctcacgcc aggcgtgcag gcgtcgggcc agcgggccgg ccagcaccac caccgccccg
     6061 acgaacagca ggatgaccgc cagcccggcg aacccg
//
LOCUS       DAOQOD010000179         6081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5072_length_6081_cov_9.773648, whole genome shotgun sequence.
ACCESSION   DAOQOD010000179 DAOQOD010000000
VERSION     DAOQOD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_6081_cov_9.773648"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..717)
                     /locus_tag="PLZ72_13610"
     CDS             complement(79..717)
                     /locus_tag="PLZ72_13610"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNA37381.1"
                     /translation="MGPLLSGLVAFAVGVNLWFLFEYLLHRFAMHELHGKGIMSREHL
                     LHHVTAGWGFTSRLLLAWLGVALVGVAAWLPLGTWLLGPAVGVGLAAGWVLGYGFYEF
                     QHAQAHLRAPRNRYERWLRKHHFHHHFGHPMANHGVTIPLWDIVFHTREAPDVVPVPR
                     RLAAGLGWLLDDDGELRAEFAADYVLVGIDRMDERQAGIDRARAFASLAPNP"
     gene            complement(755..1555)
                     /locus_tag="PLZ72_13615"
     CDS             complement(755..1555)
                     /locus_tag="PLZ72_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37382.1"
                     /translation="MSSTTPRTQPLGRRARNRVARHDQLLAAASDIVNESGLDGLTMQ
                     AVADRVDCAVGTIYTYFNSKASLIAALQANAMQTILATYHRAAEQWDDALDASGLDDP
                     AVESLVRVLAYGQLFLAGPELNPREFEFLQLAFTSPVNLVSVDDVSALTSHALTLLAE
                     GLVLIDSAVASGAISAPDPGRGDDALRRMLRWVGALEGSLLLANVRLEAMTDTDAAAF
                     DARQLGLLTSQDLLLAWGAPPRVLKAAVDELDRMRRAGSLLPRPEPRD"
     gene            1711..3183
                     /locus_tag="PLZ72_13620"
     CDS             1711..3183
                     /locus_tag="PLZ72_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNA37383.1"
                     /translation="MSAATPNAVRGIRGVAAYLPHWRLQRSAVTAVLGSNAGKGTRTV
                     AAYDEDAVTMAVEAGRRALRVAGEASVDALWFASTTGPYAEKTAAPIVHAALRLDGAV
                     PAADAGPGLRGAAAALRSALRSTDPGVLVCAGDVRTGPAGSTEEKGGGDAGAAVLVAD
                     ATSGPLAAEYLGGASATQEFLDRWRAPGELRTHAWEERFGQQRYDDLAGRAWDAALKD
                     AGVDVEQVSRVAVSSPHARAGAALAKKLGAAGVDVADRLDQSVGFTGAAHPLVLLAGL
                     VEQASPGQVVALVALADGADVFLFRVADSAAEAVAARPTVDEQVASGDDSLTYAKYLN
                     WRGVLPVQPPNRPEPARMSASAAERRLDWKYGFVGSRDRSSGALHLPPARVSFVGGAV
                     DDMDPAPMADVPATVATFTVDSLAYSPSPPIVFAVCDFDGGGRLPVELTDVTADQVSI
                     GMAVEMTFRRLNTADGIANYFWKARPAASARVAAPTEGGA"
     gene            3185..>4293
                     /locus_tag="PLZ72_13625"
     CDS             3185..>4293
                     /locus_tag="PLZ72_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNA37384.1"
                     /translation="MASHGIKDRVAIIGMGCTPFREHWDKGLDDLAVEGTHAGLDSAG
                     IGIDDVDAWWLGTAQSGMSGVTLSAPLKLQGKPVTRVENYCATGSEALRQACYAVASG
                     AYDVAAAVGVEKVKDSGYQGLNAFPIPNDGTNRTLTAAAMFSLVAPAYAEKYGVDPAE
                     LRRVLARIASKNHANGAKNPRAQFRREMDVDAICAMPAVAGMLSVFDCAGVADGAASA
                     IVVRAEDAHRYTDKPLYVKALSFVAGNASGLLDPDYDYTHFDECQWAAEDAYAQAGIT
                     DPRRQLAMAEVHDCFTPTELVLMEDLGFSARGEAWKDAEAGVFDLHGELPVNTDGGLK
                     SFGHPVGASGLRMLFECWLQLRGEAPEDRRIDTADR"
     assembly_gap    4294..4393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4394..4753
                     /locus_tag="PLZ72_13630"
     CDS             <4394..4753
                     /locus_tag="PLZ72_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37385.1"
                     /translation="TPAVFAVQVAGFDGLVGSDPVGAQRALRELEGRLDRVVRGRDVL
                     GFEPPARFLVGCGGLAPDAAGGVLERIRGGAAFPVDIGGDAVSLVLDVGSAYFAEGAT
                     GESLVVDAESDLGRLVD"
     gene            4768..5229
                     /locus_tag="PLZ72_13635"
     CDS             4768..5229
                     /locus_tag="PLZ72_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNA37386.1"
                     /translation="MPRLLVVHHTPSPALHSLLTAVLDGVADPALADVEVVTRPALVA
                     SAADVLEADGVVVGGPVNLGYLAGALKHFFDQIYYPCLEDTVGRPFAAYLHGNDDLTG
                     GLVALEKITTGLRWRRVQPVLGVTGAPSLDDLAACRELGAVTAATAAGLLG"
     gene            5387..>6081
                     /locus_tag="PLZ72_13640"
     CDS             5387..>6081
                     /locus_tag="PLZ72_13640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA37387.1"
                     /translation="MAAIIPEGSIVWGLQLPVQSKSTAYRQAWELDAGPADLATVATA
                     ADRAGAFYVAVCDHVAIPRPADEVMSDTWYDTIATLGWLAAQTERVQLVSHVYVLPYR
                     HPLQVAKSFVTLDALSGGRAVLGAGAGHLESEFALLGVDYDGRGRVVEEALPVIRAAF
                     AEEYPTVGGPGAEVGVGVAPRPVRPGGPPIWIGGSSPAAMRRAAVLADGWLPQGPPKE
                     GMKAGIARIRDRRA"
BASE COUNT          803 a         2162 c         2201 g          815 t
ORIGIN      
        1 tgcaccagcg cggtgatcac cgcgctggtg catgacaaag cgggggtgtg gcggcgttcg
       61 tccggctcgt ccgggccgtc aggggttcgg cgcgaggctg gcgaacgccc gcgcccggtc
      121 gatgcccgcc tgacgttcgt ccatccggtc gatcccgacc agcacgtagt cggcggcgaa
      181 ctccgctcgc agctcgccgt cgtcgtcgag cagccaaccc aacccggcgg ccaggcggcg
      241 tgggaccggc accacgtcgg gtgcctcacg ggtgtggaag acgatgtccc acaacgggat
      301 cgtcaccccg tggttggcca tcgggtgccc gaagtggtgg tggaagtggt gctttcgcag
      361 ccagcgctcg tagcggttcc gcggtgcccg cagatgggcc tgggcgtgtt ggaactcgta
      421 gaagccatag ccgagcaccc acccggcggc caggccgact ccgacggcgg gaccgagcag
      481 ccaggtgccg agcggcagcc acgccgcgac gccgaccagc gcgacgccca gccacgccaa
      541 caggagacgg gaggtgaaac cccatccggc cgtcacgtgg tgcagcaggt gctcacggct
      601 catgatcccc ttgccgtgca actcgtgcat ggcgaagcgg tgcagcaggt actcgaacag
      661 gaaccagagg ttgaccccga ccgcgaaggc caccagcccg ctcagcaggg gacccatggc
      721 acctctgtcg cgatccggcg ggccggtgcc ggggtcagtc ccgcggctcg ggccggggga
      781 gcagcgatcc ggccctgcgc atgcggtcca gctcgtcgac cgcggccttg aggacccggg
      841 gcggagcgcc ccaggcgagc agcaggtcct ggctcgtcag cagcccgagc tgccgtgcgt
      901 cgaacgcggc ggcgtcggtg tcggtcatcg cctcgagccg tacgttggcc agcagcaggc
      961 tgccctcgag cgctccgacc cagcggagca tccgacgaag ggcgtcgtct ccccgtccgg
     1021 gatcgggggc ggagatcgca ccggaggcca ccgcggagtc gatgaggacc agcccttcgg
     1081 ccagcagggt caacgcgtgc gaggtcagcg cgctcacgtc gtcgaccgac accaggttga
     1141 ccggcgaggt gaaggcgagc tggaggaact cgaactcgcg ggggttgagc tcgggcccgg
     1201 ccaagaacag ctggccgtag gccagcaccc gcacgagcga ctcgacggcg gggtcgtcga
     1261 gccccgacgc gtcgagggcg tcgtcccact gctcggcggc ccggtggtag gtcgccagga
     1321 tggtctgcat ggcattggcc tgcagcgcgg cgatcagcga cgccttggag ttgaagtacg
     1381 tgtagatggt gccgaccgcg cagtccaccc ggtcggcgac cgcctgcatg gtcagaccat
     1441 cgaggcccga ctcgttgacg atgtcgctgg cggcggcgag cagctggtcg tggcgggcga
     1501 cccggttgcg ggcgcggcga ccgagcggct gggtccgagg tgtggtggag ctcaccggac
     1561 gctcgaacgg gacggaacgg ggttcacgcg catctgtcca tgctcggccc gtgacctcgc
     1621 ccgtgtcaat gatcctcccg ccgagcctcg ccgctcgcgg cggacggcca cgacttctga
     1681 cggtgcgtca gatgtcccta cactccaccc atgagcgcag ccaccccgaa cgcggtccgc
     1741 ggcatccgcg gcgtcgccgc ctacttgccg cactggcgcc tgcagcgatc ggccgtcacc
     1801 gcggtgctcg gatcgaacgc cgggaagggc acccgtaccg tcgccgccta cgacgaggac
     1861 gcggtcacca tggccgtcga ggccggccgg cgggctctgc gcgtcgcggg ggaggcgtcc
     1921 gtggacgcgc tgtggttcgc ctccaccacc ggtccgtacg ccgagaagac cgcggcgccg
     1981 atcgtgcacg ccgcgctccg gctggacggc gcggtgcccg ccgcggacgc cggacccgga
     2041 ctccgcggcg cggccgcggc gcttcgctcg gcgctgcgct ccaccgaccc tggcgtgctc
     2101 gtctgcgccg gcgacgtccg caccggcccc gccggctcga ccgaggagaa gggcggcggc
     2161 gacgccggcg cggcggtcct ggtggcggac gcgacctcgg gcccgctggc cgccgagtac
     2221 ctcggcggcg cgtcggccac ccaggagttc ctcgaccgct ggcgggcccc cggcgagctg
     2281 cgcacccacg cctgggagga gcgcttcggc cagcagcggt acgacgacct ggccggacgg
     2341 gcctgggacg cggcgctcaa ggacgccggc gtcgacgtcg agcaggtgtc gcgggtggcg
     2401 gtgtcgtccc cgcacgcccg ggccggcgcg gcgctggcca agaagctcgg cgctgccggc
     2461 gtcgacgtgg ccgaccggtt ggaccagagc gtcggtttca ccggcgccgc acacccgctc
     2521 gtgctgctcg ccggtctggt cgagcaggcg tctcccggtc aggtcgtcgc gctggtcgcg
     2581 ctggccgacg gcgcggacgt gttcctgttc cgggtcgccg actccgcggc cgaggcggtg
     2641 gcagcccggc ccaccgtcga cgagcaggtc gcgtcgggcg acgactcgct gacctacgcc
     2701 aagtacctga actggcgcgg cgtgctgccg gtccagccgc ccaaccggcc cgagccggcc
     2761 cgcatgtcgg cgtcggccgc ggagcggcga ctggactgga agtacggctt cgtcggcagc
     2821 cgggaccgtt ccagcggtgc gctgcacctg ccgccggcac gggtcagctt cgtgggcgga
     2881 gcggtcgacg acatggaccc ggcgccgatg gccgacgtgc ccgccaccgt cgccaccttc
     2941 accgtcgact cgctggccta ctcgccgtcg ccgccgatcg tgttcgcggt gtgcgacttc
     3001 gacggtgggg gacgcctgcc cgtcgagctc accgacgtca ccgccgacca ggtctccatc
     3061 ggcatggcgg tcgagatgac cttccgacgc ctcaacaccg cggacgggat cgccaactac
     3121 ttctggaagg cccgcccggc cgcatcggcc cgggtcgccg caccgaccga gggaggggcc
     3181 tgacatggcc agccacggca tcaaggaccg cgtcgccatc atcggtatgg ggtgcacccc
     3241 gttccgcgag cactgggaca aggggctcga cgacctggcg gtcgagggga cccacgccgg
     3301 gctcgactcc gcgggcatcg gcatcgacga cgtcgacgcc tggtggctcg gaaccgccca
     3361 gtccggcatg tcgggcgtga cgctgtcggc cccgctgaag ctgcagggca agccggtcac
     3421 ccgggtcgag aactactgcg ccaccgggtc cgaggcgctg cgccaggcct gctacgcggt
     3481 ggcgtcgggc gcctacgacg tggccgccgc ggtcggcgtc gagaaggtca aggactcggg
     3541 ctaccagggc ctcaacgcgt tccccatccc caacgacggc accaaccgga ccctcaccgc
     3601 ggcggccatg ttctcgctcg tcgcccccgc ctacgccgag aagtacggcg tcgatccggc
     3661 cgagctgcgt cgggtgctgg cccgcatcgc cagcaagaac cacgccaacg gcgccaagaa
     3721 cccgcgggcg cagttccgcc gcgagatgga cgtcgacgcc atctgcgcca tgcccgcggt
     3781 cgcggggatg ctgtcggtgt tcgactgcgc cggcgtggcc gacggcgcgg cgagcgccat
     3841 cgtcgtgcgg gccgaggacg cgcaccgcta caccgacaag ccgctctacg tgaaggcgct
     3901 gagcttcgtc gccggcaacg cgtccggcct gttggacccg gactacgact acacgcactt
     3961 cgacgagtgc cagtgggccg ccgaggacgc gtacgcccag gccggcatca ccgacccgcg
     4021 ccgtcagctg gccatggccg aggtccacga ctgcttcacg ccgaccgagc tggtcctgat
     4081 ggaggacctc ggcttctcgg ctcggggcga ggcgtggaag gacgccgaag ccggcgtgtt
     4141 cgacctccac ggcgagctgc cggtcaacac cgacggcggc ctcaagtcct tcggccaccc
     4201 ggtcggcgcg tccgggctgc ggatgctgtt cgagtgctgg ctccagctgc gcggcgaagc
     4261 tcccgaggac cgtcgcatcg acaccgcgga ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnacccccg cggtgttcgc ggtgcaggtc gccggcttcg acgggttggt
     4441 cggatctgat cccgtcgggg cgcaacgggc actccgggag ctcgagggac gactggaccg
     4501 ggtggtgcgc ggtcgtgacg tgctcggctt cgagccgccg gcccggttcc tggtcggttg
     4561 cggcggcctg gcccccgacg ccgccggagg cgtcctggag cggatccgtg gcggcgccgc
     4621 cttccccgtc gacatcggcg gcgacgcggt gtcgctggtg ctcgacgtcg gatccgcgta
     4681 cttcgccgag ggcgcgaccg gggagtcgct ggtcgtcgac gccgagtcgg acctgggccg
     4741 actcgtcgac tgagcagggt tcctgcggtg ccgcggttgc tcgtggtgca ccacacgccg
     4801 tcgccggccc tgcactcgct gctcaccgcg gtgctcgacg gcgtggccga cccggcgttg
     4861 gccgacgtcg aggtggtgac ccgaccggcg ctcgtcgcct cggccgcgga cgtgctcgaa
     4921 gccgatggcg tggtggtcgg cggcccggtg aacctgggct acctggccgg tgcgctcaag
     4981 cacttcttcg accagatcta ctacccgtgc ctggaggaca ccgtcggacg gccgttcgcc
     5041 gcctacctgc acggcaacga cgacctgacc ggcggtctcg tcgctctgga gaagatcacg
     5101 accgggctgc ggtggcgtcg ggtccaaccg gtgctcgggg tcaccggcgc tccgtcgctc
     5161 gacgacctgg ccgcgtgccg cgagctcgga gccgtcaccg cggcgaccgc ggccggactc
     5221 ctcgggtagg ccctgctcgc ctggcggagc cgagatcgcc ggacagcggc tgctctgacc
     5281 cgggaggtcg cggctggtcc gtaggatcgg cgccgtacgc catcgtcacc gcccgcgccg
     5341 cggggtcgga cccgcgacgg caccaggggc agacaggaga ccgaccgtgg ccgcgatcat
     5401 ccccgagggc agcatcgtgt ggggcctaca gctaccggtg cagtccaagt cgaccgcgta
     5461 ccgccaggca tgggagctcg acgccggacc cgcagatctg gcgaccgtcg ccaccgccgc
     5521 ggaccgcgcg ggtgcgttct acgtggcggt ctgcgaccac gtcgccatcc cccggccggc
     5581 cgacgaggtc atgtccgaca cgtggtacga cacgatcgcc acgctcggat ggctggcggc
     5641 gcagaccgag cgggtccagc tggtcagcca cgtctacgtg ctgccgtacc gtcacccgct
     5701 gcaggtcgcc aagagcttcg tgaccctgga cgccctgtcg ggaggccggg cggtgctcgg
     5761 cgccggcgcc ggtcacctcg agagcgagtt cgcactgctc ggggtggact acgacggccg
     5821 gggtcgagtg gtcgaggaag ccctgccggt gatccgcgct gccttcgccg aggagtaccc
     5881 gaccgtcgga ggtcccggcg ccgaggtcgg tgtcggggtc gcgccccgtc cggtgcgtcc
     5941 ggggggtccg ccgatctgga tcggcgggtc gtcgccggcg gccatgcgcc gcgccgccgt
     6001 gctcgccgac gggtggctgc cgcagggacc tcccaaggag gggatgaagg ccggcatcgc
     6061 ccgcatccgc gatcgccgcg c
//
LOCUS       DAOQOD010000180         6077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5077_length_6077_cov_9.314846, whole genome shotgun sequence.
ACCESSION   DAOQOD010000180 DAOQOD010000000
VERSION     DAOQOD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_6077_cov_9.314846"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLZ72_13645"
     CDS             complement(<1..266)
                     /locus_tag="PLZ72_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37388.1"
                     /translation="MGFLDKAKGLADQAMAKADQAMAGGPTSKSADPYFRDLGVLAYL
                     EATGRAPAEIDRLRQETMTAIQGLEGQTALNFAMSSAAPPPPGAA"
     gene            485..1213
                     /locus_tag="PLZ72_13650"
     CDS             485..1213
                     /locus_tag="PLZ72_13650"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNA37389.1"
                     /translation="MDDTTTPDGAGPVTVTVTDGVAVIDLDDGKANALGFDVLAGLES
                     ALDRAEADDVSAVVIVGREGKFSAGFDLKVMTSGPEGARDMLGRGALLGLRMYMFPKP
                     VVLAVTGHALAMGAILLCCADVRVGADGPFKIGLNEVAIGMPVPGFAVGICQDRLAIP
                     AFTAAIQLARIHSPAEALAAGFLDEVVDVAAVRDRAVEVAAELGTRLHRGPFRVTRET
                     VRGPVAERLRSELAADLSVFDVSS"
     gene            complement(1305..3503)
                     /locus_tag="PLZ72_13655"
     CDS             complement(1305..3503)
                     /locus_tag="PLZ72_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37390.1"
                     /translation="MTVRLRLSALLTALVVVAVGCQHAEAPAPAISGDIDKGNPTTTE
                     PAAVQISTDTPTPEPLSPERQARLNAALATIPAGCEILSDKSCLLPFPSDAHTVADDS
                     TGTHKRINLPSGQLVNVDGVPLDPTAWNMNDGWSPSTPILAFVPGLDPSKTALPSEGD
                     IGFSVTEESATVIVDLTTGQLVPHWAEMDSRATSDAERLLILRPATSLIETHQFAVAF
                     RHLIGTNGAPLPAPITFQAIRDNNATGNARVEARQRDFNLVFPKLATAGVAREGLYLA
                     WTFTVASPQSLAGRVLSMRDDAFGKVSGTAPVFTVTETQTDDLQPGIAKVVRGTFEVP
                     LYLTGDGGPGSRMTFSPLNGQPISTGTTTANFTCSLPVKGIKEGNSVPVVYGHGLLGS
                     ADEAASSQVQHTAAANNSVYCATDIVGMAEGDTANAAAALADINLFPSIPDRLQQAML
                     NTLFLGRLLTNVDGLGSAQQFQTTGGANMLNNAQAYYDGNSQGAIFGGAVTAVSNQWT
                     KAVLGVGGMNYSTLLDRSVDFNRFLPVLTGAYPDPIDQQLIFGVLQMLWDRGETSGYV
                     QHLTDRAYDRTPKHQVLMTVAFGDHQVATITADNIARTLKLPIYEPTLPKKTEPMQGV
                     TTSQNDFFYNLGPIRKFPLDGSALYYWYQGTLAPPLGNITPEMSAAYQAQCQGAAAET
                     ERQCEDPHEMPRRQPQVIAQKKAFFQDEGVITNVCKDKPCLGKPRSEFDY"
     gene            complement(3597..4616)
                     /locus_tag="PLZ72_13660"
     CDS             complement(3597..4616)
                     /locus_tag="PLZ72_13660"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNA37391.1"
                     /translation="MTTSPRLLRRRSPSVRSPFRRRIGVAAVAASVLVGFAAPVGSEP
                     LAGADPVAVGRQKVASATAARQRAESRLAQVVSRRTALEQRSRELDGADAASTEALAG
                     ARRRVRELAVAAFIDGGRTELLQASLTPDEAAVVAWRVGMVSGGADTVSDAVDHFEAL
                     RSANDPEQEQVASELDAVRSEEEQARSDLVQSSAAERDATAALDASRREQAAARAARA
                     SSAAVAPTPRAPAAPGRRSTARPSPSSSSAAGLGVRSSGGAPTAEEAAFLARVRQCES
                     RGNYQIVSSTGRYRGAYQFSVETWRGVGGSGDPAAASPAEQDARALALLRLQGKRAWP
                     VCSGR"
     gene            complement(4845..5330)
                     /locus_tag="PLZ72_13665"
     CDS             complement(4845..5330)
                     /locus_tag="PLZ72_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37392.1"
                     /translation="MAAYDPKRPRPRADGEEPAPVEALLDPDPAEPVAVAEPAHADAV
                     AEPAHADAVAEPAEVRYVVELEDSDPAPADEPPADEAPPEPAADPAPVTVDEPAGAGT
                     TSTPSATDRPDLRRAGSDVPVAEPPPEPTANRAVVVVGAAGAGLLTLLVLLLLLRRRR
                     R"
     gene            complement(5330..>6077)
                     /locus_tag="PLZ72_13670"
     CDS             complement(5330..>6077)
                     /locus_tag="PLZ72_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37393.1"
                     /translation="VFDAATTIDEVLAEDRAGAASAAVRRSGWEPAVGHAPDLVPSSP
                     RLAQREHQLATDPIGAQDRLRVALDGLADRYDDVIVDCPPSLGLLTVNALFAADRVLI
                     VAEPAAWSLDGVEQILRNVRRIADRQSGRPEVAGIVVNRLGRTRDAGYWFAQLQETHG
                     DLLLEPAISARAAVTEAAAQSMPIHLLRRDGAEEAGDQFAALARALESRPAADAVTSL
                     PTERLDLTAELVGSDVVVPAAGVAGSSRDL"
BASE COUNT          796 a         2078 c         2329 g          874 t
ORIGIN      
        1 gcagcgcccg ggggcggcgg cgcggccgac gacatggcga agttcaacgc cgtctggccc
       61 tccaggccct ggatcgccgt catcgtctcc tggcggagcc gatcgatctc ggccggcgca
      121 cggccggtgg cctcgaggta ggccaggacg ccgaggtcac ggaagtaggg gtcggccgac
      181 ttgctcgtcg gcccaccggc catggcctga tcggccttgg ccatggcctg gtcggccagg
      241 cccttcgcct tgtcgaggaa tcccatctgt gaacctccgg gtcgttcgcg ttcggtcggg
      301 tcaccgctgg tgcgggtcgt gatcgtgggg gcaggctccg ccgcaccgtg ggctcacgga
      361 cacccgacgg ggcctcgccg gtcagcgtag cgagggcgaa gccgatccgg acgcgccccg
      421 agcaccgtgt gccacgccgc tgcgggctcc gggacgagcc gggggccggc actagcgtgc
      481 ggccatggac gacaccacca cccccgacgg cgccggcccg gtcaccgtca ccgtgaccga
      541 cggcgtcgcc gtcatcgacc tcgacgacgg caaggccaac gcgttgggct tcgacgtgct
      601 cgccgggctc gagagcgccc tcgatcgcgc cgaggccgac gacgtctccg ccgtcgtcat
      661 cgtcggccgg gaggggaagt tctccgccgg cttcgacctg aaggtgatga cctcgggtcc
      721 cgagggcgca cgcgacatgc tcggacgcgg agccctgctc ggcctgcgga tgtacatgtt
      781 ccccaagccg gtcgtgctgg ccgtgaccgg gcacgcgctg gcgatgggcg ccatcctcct
      841 gtgctgcgcg gacgtgcgcg tcggcgccga cggcccgttc aagatcggcc tcaacgaggt
      901 ggcgatcggc atgccggtgc ccggcttcgc ggtcggcatc tgccaggacc gactggcgat
      961 ccccgcgttc accgcggcca tccagctggc ccggatccac tcgccggccg aggcgctcgc
     1021 cgccgggttc ctcgacgagg tggtcgatgt ggcagcggtc cgcgaccggg ctgtcgaggt
     1081 cgccgccgag ttggggaccc gcctgcaccg cggaccgttc cgcgtgacgc gcgagacggt
     1141 gcggggtccg gtggccgagc ggctccgctc cgagctcgcc gcggatctca gcgtgttcga
     1201 cgtcagcagc tgagcggcac gactccggcc ctggacctct gtggcccggc cgcccgacgg
     1261 cgaccgggcc acagccggaa caggtcagga gtggacgggt ccgatcagta gtcgaactcg
     1321 ctgcggggct tgcccaggca gggcttgtcc ttgcagacgt tggtgatcac gccctcgtcc
     1381 tggaagaacg ccttcttctg ggcgatgacc tgcggctgac gccgcggcat ctcgtgggga
     1441 tcttcgcact gccgttcggt ctcggccgcc gcgccctggc actgggcctg gtaggccgcg
     1501 ctcatctccg gcgtgatgtt gccgagcggc ggggccaggg tgccctggta ccagtagtag
     1561 agggcggagc cgtcgagcgg gaacttgcgg atgggcccga ggttgtagaa gaagtcgttc
     1621 tgcgaggtgg tgacgccctg catgggttcg gtcttcttcg gaagcgtcgg ctcgtagatc
     1681 ggcagcttca gggtgcgggc gatgttgtcg gcggtgatcg tcgccacctg gtggtcgccg
     1741 aacgcgaccg tcatcagcac ctggtgcttg ggcgtgcggt cgtaggcccg gtcggtcagg
     1801 tgctggacgt agccgctggt ctcaccccgg tcccacagca tctgcaggac gccgaagatc
     1861 agctgctggt cgatcgggtc cgggtaggca ccggtcagca cgggcaggaa ccggttgaag
     1921 tcgacgctgc ggtcgaggag cgtcgagtag ttcatgcccc cgacgccgag caccgccttg
     1981 gtccactggt tggacaccgc ggtgaccgcg ccgccgaaga tggcgccctg gctgttgccg
     2041 tcgtagtacg cctgcgcgtt gttcaacatg ttggcgccgc cggtggtctg gaactgctgc
     2101 gccgagccca gcccgtcgac gttggtcagc agccggccga ggaacagcgt gttgagcatg
     2161 gcctgctgca gccggtccgg gatcgacggg aacaggttga tgtcggccag agccgccgct
     2221 gcgttggcgg tgtcgccctc ggccatgccg acgatgtcgg tcgcgcagta caccgagttg
     2281 ttggccgctg cggtgtgctg cacctgcgag ctggcggctt cgtcggccga gccgagcagt
     2341 ccgtggccgt acaccaccgg gacggagttg ccctccttga tccccttgac cggcagcgag
     2401 cacgtgaagt tcgcggtcgt cgtccccgtc gagatcggct ggccgttgag ggggctgaag
     2461 gtcatccggg atcccggtcc gccgtcgccg gtcaggtaca gcgggacctc gaaggttccg
     2521 cgcacgacct tggcgatccc gggctggagg tcgtcggtct gggtctcggt gacggtgaac
     2581 accggcgcgg tgcccgacac cttgccgaag gcgtcgtcgc gcatggacag gacccggccg
     2641 gccagcgact ggggcgaggc gacggtgaag gtccacgcca ggtagaggcc ctctcgggcg
     2701 acaccggcgg tggcgagctt ggggaacacc aggttgaagt cgcgctggcg ggcctcgacg
     2761 cgggcgttgc cggtggcgtt gttgtcgcgg atggcctgga aggtgatggg ggccggcagc
     2821 ggtgccccgt tggtgccgat caggtggcgg aacgccaccg cgaactggtg cgtctcgatc
     2881 agcgaggtgg ccggccgcaa gatcagcagg cgctccgcgt cgctggtcgc ccgggagtcc
     2941 atctcggccc agtgcgggac gagctgaccg gtggtgaggt cgacgatgac ggtggcggac
     3001 tcctcggtga ccgagaagcc gatgtcaccc tcggacggga gcgccgtctt ggaggggtcc
     3061 aggccgggga cgaaggcgag gatcggggtg gaggggctcc agccgtcgtt catgttccag
     3121 gcggtcgggt cgagcgggac gccgtcgacg ttgaccagct gacccgacgg caggttgatc
     3181 cgcttgtgcg ttcctgtcga gtcgtcagcc accgtgtggg cgtcgctcgg gaacggcagc
     3241 aggcagctct tgtcgctcag gatctcgcat ccggcgggga tggtggcgag agcggcgttc
     3301 agccgggcct ggcgctcggg gctcagcggc tcgggcgtcg gcgtgtcggt ggagatctgc
     3361 accgccgccg gctcggtcgt cgtcgggttg cccttgtcga tgtcgccgct gatcgccggc
     3421 gcgggcgcct cggcgtgctg gcaaccgacg gcaaccacga cgagggcggt cagcagtgcg
     3481 gagaggcgga gtcggacggt cacagcgttc ctcggtctcg gccggtcata ccgcgggcga
     3541 ccctagccaa agcacggcgt ccgcgggaaa cccacggcaa tggcgtcccc atcggctcaa
     3601 cggccgctgc acaccggcca ggcgcgcttg ccctggagcc gcagcagggc gagggcccgg
     3661 gcgtcctgct cggcgggcga cgccgcagcc ggatcaccgg acccaccgac ccctcgccac
     3721 gtctcgacgc tgaactggta ggcaccccgg tagcggccgg tcgagctcac gatctggtag
     3781 ttgccacggg actcgcactg gcggacccgg gccaggaagg cggcctcctc agcggtgggg
     3841 gctcctcccg agctccgcac cccgagaccg gccgcgctgg acgacgacgg ggacgggcgt
     3901 gcggtcgaac gccgacccgg ggcggcaggg gcccggggcg tcggagccac agccgccgag
     3961 gatgcccgag cggcccgtgc cgcggcctgc tcacggcgac tcgcgtcgag cgcggcggtg
     4021 gcgtcgcgct cggcggctga gctctggacc aggtcgctgc gggcctgctc ctcctcgctt
     4081 cgcaccgcgt cgagctcgga ggcgacctgc tcctgttcgg ggtcgttcgc cgaccgcaac
     4141 gcctcgaagt ggtcgaccgc gtccgacacc gtgtctgctc cgccgctcac catgccgacc
     4201 cgccaggcca cgacggcggc ctcgtcagga gtcagagagg cctggagcag ttcggtgcgc
     4261 cctccgtcga tgaaggcggc gaccgcgagc tcccggaccc gtcggcgggc tccggccagc
     4321 gcctcggtcg aggccgcgtc agctccgtcg agctctcgcg atcgctgctc gagcgccgtg
     4381 cgccgcgaca ccacctgggc caggcgggat tcggcccgct ggcgagccgc agtcgccgac
     4441 gccaccttct ggcgaccgac cgcgaccggg tcggccccgg cgagcggctc cgaacccacg
     4501 ggcgccgcga accccaccag gacggaggcc gcgaccgcgg ccacaccgat gcggcggcgg
     4561 aacggggatc ggaccgaggg cgatcggcgg cggaggagtc gtggcgaggt ggtcatgagg
     4621 gcggtggagg ctgcgccggt cgcgcccctg aggtgacaga tccgcgacac agggcgggca
     4681 gtctgtcatc ggagcgtcac gacggaaagg tgacggaaag acgatcgtca cctgatcggc
     4741 acggacgggc cgcgggttga gcgcgcgagc cgtcccggga tccggggatc gatgtggccg
     4801 acccgcagtc ccggaccgac gcgaccgggt cggcggttgc ccggtcagcg acgacggcgg
     4861 cgcagcagca ggagcaggac cagcaacgtc agcagacccg cgccggccgc ccccacgacc
     4921 acgaccgccc ggttggcggt cggctccggc gggggttcgg ccaccggcac gtcggatccg
     4981 gcccgccgca ggtccgggcg atccgtcgcc gacggggtgg acgtggtccc cgctcccgcg
     5041 ggctcgtcga cggtaactgg cgccgggtcg gccgcgggct cgggcggagc ctcgtcggcc
     5101 ggaggctcgt cggccggagc gggatcggag tcctccaact cgacgacgta gcggacctcg
     5161 gccggctcgg cgaccgcatc tgcgtgggcc ggctcggcga ccgcatctgc gtgggccggc
     5221 tcggcgactg cgaccggttc ggcggggtcg gggtcgagca gcgcctcgac cggcgccggc
     5281 tcctcgccgt cggcgcgggg ccgggggcgc ttggggtcgt aggccgccat cagaggtctc
     5341 tcgatgagcc ggcgacgccg gccgcgggga cgacgacgtc ggagccgacg agctcggcgg
     5401 tgaggtccag ccgttcggtc ggcagggagg tgaccgcgtc ggccgccggt cgggactcca
     5461 gggcccgcgc caaggcggcg aactggtcgc cggcctcctc ggcgccgtca cggcgcagga
     5521 ggtggatggg catcgactgc gccgctgcct cggtgaccgc ggcgcgggcc gagatggcgg
     5581 gctcgagcag caggtcgccg tgggtctcct gcagttgggc gaaccagtag ccggcgtcac
     5641 gggtgcggcc cagccggttg acgacgatcc ccgccacctc gggtcgaccg ctctgacgat
     5701 cggcgatccg gcggacgttg cggaggatct gctcgacgcc gtcgaggctc cacgccgccg
     5761 gctcggcgac gatcagcacc cggtccgccg cgaagagggc gttgacggtc agcaggccga
     5821 gcgacggcgg gcagtcgacg atgacgtcgt cgtagcgatc agccagaccg tcgagggcga
     5881 cccgcagtcg gtcctgggcg ccgatcgggt cggtcgcgag ctggtgctcg cgctgggcca
     5941 ggcgcggcga gctcggcacc aggtccggag cgtggcccac cgccggctcc caccccgagc
     6001 ggcgcaccgc ggcagaagcg gcaccggccc ggtcctcggc gaggacctcg tcgatggtgg
     6061 tcgcggcatc gaacacc
//
LOCUS       DAOQOD010000181         6019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5159_length_6019_cov_9.125587, whole genome shotgun sequence.
ACCESSION   DAOQOD010000181 DAOQOD010000000
VERSION     DAOQOD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_6019_cov_9.125587"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLZ72_13675"
     CDS             <1..459
                     /locus_tag="PLZ72_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37394.1"
                     /translation="PVSLAELLVVSARRSLLAATARVRGLDQFRHLARRQAARLWMVH
                     LALMVTLVLASTWLLPVVSPAEPTWTEALALVAAFGLLGTVLTAGLLLLAAGRLASVS
                     GLLWGGAAPVLLIRLWADGWSPAVLVVLAIVAAVGAFMSSMATEKLFSYR"
     gene            558..1367
                     /locus_tag="PLZ72_13680"
     CDS             558..1367
                     /locus_tag="PLZ72_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNA37395.1"
                     /translation="MRAVLMVGGRGMRLRPFTTTIPKPLVPVGGELPIIELLLRQLRR
                     CGVTEVTLATGHMGGLIQRYVGDGAQWDLTIDYWHEDAPLGTIGPLLQHRDSLPDDVL
                     LLNGDLLCSIDFAELVAFHTRQGGGLTMAGSTRRIDVEFGVLEVDGTELNEFREKPQL
                     AYLANMGIYVLSRTVLDQLDGDGAHGVDELIRSLLAAGVHPNVYEFDGYWLDIGRPED
                     YDRANLEFAELRRQLLGDEVDTPDSTIDLRPLRPDPHPDAVPLDAIVGSVA"
     gene            1552..3501
                     /locus_tag="PLZ72_13685"
     CDS             1552..3501
                     /locus_tag="PLZ72_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37396.1"
                     /translation="MKATSNNRKARGAPRRRSLVIALAVALIAAVAPSLTASVGAADP
                     AKPVSMRLLVIAADGNETDYPAITTFLDEVGVPYDKILAADTPLTASMLSDGTTGKYQ
                     GIVLTTGNLTYFDAGAGTWQSAFSPDEWATLRQYQKDFHVRSVTSYTFPEASYGLSYA
                     GYRNTLGSELPATLTAAGQAVWPYVNAANPIELTGAWVYLGSIIDPSVTTPLVNVTVD
                     GTTYPVASVTRFDGYENLAVTAANNPNLLHSILLSTGWINWVSGGNFLGNRSSSIDVQ
                     IDDLFIGTDQWDPATNTAHTEYTNVPADVTAMVAYQNARRANPATPGFKVEYAFNGAG
                     GPTNPSLVNTVRANRSQFGFINHTWTHFNLDCGDCPNPTGQITTNTTQIRSQILTNRM
                     YGALLGLPGNTDTMVQPDISGINTPPNPMAQKAAGGVGIRFWIGDTSRAGQTNPTFNT
                     GFATAGDSRVFVVPRRPTNLFVAATTPDQWTSVYNHFYAPGGILCGMTTCFDRPQTYQ
                     EILDHESDYLLRYLLRGDLDPWMFHVGNTRAYSGNRSVLSDLLDETFSKYSSMVNTPV
                     RSVSFKQAGQAMQGRAAYNAAGVTATVTPCTSITVKATKAATVPVNGVAYTAANSTVE
                     AYGGRTISQVRLAAGQTVTIPLPAC"
     gene            4071..5444
                     /locus_tag="PLZ72_13690"
     CDS             4071..5444
                     /locus_tag="PLZ72_13690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNA37397.1"
                     /translation="MAVQGLGFVGAAMSIAVASALDDRGEPAFDVVGVDRDDPAGRER
                     IDALNSGRFPFPTSDDHLRSALAAVRGRGNLVAGSDPAAYALADVVVIDVPLDIDWRA
                     EPPELLLEGFTSAIRTVGRYIEPGTLVLVETTVPPGTTERVVVPALAEELVARGHRPD
                     DVLVAHSYERVMPGAEYLASIVNYWRVYAGRTEAAAKAAEAFLSQVINVAEYPLTRLS
                     NTTASETAKVLENTFRATTIALMDEWGRFAETVGIDLVEVVDAVRMRPTHANVRTPGF
                     GVGGYCLTKDPLFARLASEVLFDSPVEFPFSTAAVRVNDQAPVRALDRLEELMGRPVT
                     GRHVLLLGVSYRQDVGDTRYSPSTTFVEAAAARGARVSAHDPLVDRWEELDRPVMTDL
                     PPLDDVDAVVLAVPHDDYRRLDVTTWLGEHRPVLLDAFDVWTDDQRRAAEALGCTVGS
                     IGRGTPP"
     gene            5500..>6019
                     /locus_tag="PLZ72_13695"
     CDS             5500..>6019
                     /locus_tag="PLZ72_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA37398.1"
                     /translation="MSGRALITGGAGFIGAHLSRRLLDDGWEVTLVDNLSRGVQDTAL
                     ASLRERPGFALHVADLRDPSTLGSIPTDHTHVLHFAALLGVQNVIDRPSAVLRENVAL
                     LETMLDVASAQSSLERFLFPSTSEVYAGTQQFHDLPFPTPEDTPLTVSALDRPRTSYM
                     LSKIYGEALVHHS"
BASE COUNT          953 a         2171 c         1971 g          924 t
ORIGIN      
        1 ccggtctcgc tggccgagct gctcgtcgtg agcgcacgcc gctccctcct cgcagcgacg
       61 gcccgggtgc gggggctgga ccagttccgt cacctcgccc ggcggcaggc ggctcgcctg
      121 tggatggtgc acctcgccct gatggtcacg ctggtgctcg cgagcacgtg gctgctccca
      181 gtcgtgagcc cggccgagcc gacatggacg gaggccctgg cgctggtggc cgcgttcggc
      241 ctgctcggaa cggtgctcac cgcgggactg ctcctgttgg ccgccgggag gctcgccagc
      301 gtgtcggggc tgctgtgggg tggagccgca ccggtgctgc tgatcaggct ctgggccgac
      361 ggctggtcgc cagccgtcct ggtcgtcctc gcgatcgtcg ccgccgtggg agcgttcatg
      421 tcgtcgatgg cgaccgagaa gctcttctcc taccgctgag cgcggttccc gccgcgcggt
      481 cagcggtgcg gcttgactgc gttggggaga gccacaagga tgcgaaacgg acaactcatg
      541 ctggggaggg aagccctatg agagctgtgc tgatggtcgg cggacgcggg atgcgtctgc
      601 ggcctttcac cacgacgatt ccgaagccac tggtgccggt cggcggcgag ctgccgatca
      661 tcgagctgtt gcttcgtcag cttcgccgct gcggcgtcac cgaagtcacg ctggccaccg
      721 ggcacatggg tggactgatc cagcgctacg tcggcgacgg cgcccagtgg gacctgacga
      781 tcgactactg gcacgaggac gcaccgctgg gcacgatcgg tcccctgctc cagcacaggg
      841 actcgctccc cgacgacgtc ctgctgctca acggcgacct gctgtgcagc atcgacttcg
      901 ccgagctggt cgcgttccac acgcgacagg gcggcgggct caccatggcg ggatcgaccc
      961 gacgcatcga cgtcgagttc ggcgtgctcg aggtcgacgg caccgagctc aacgagttcc
     1021 gtgagaagcc gcagctcgcc tacctggcca acatggggat ctacgtgctg agccgcaccg
     1081 tgctcgacca gctcgacggg gatggggcgc acggcgtgga cgagctcatc cgctcgctcc
     1141 tcgcggcggg ggtccacccc aacgtctacg agttcgacgg ctactggctc gacatcggtc
     1201 gacccgagga ctacgaccgg gccaacctcg agttcgccga gctgcggcga cagctcctgg
     1261 gcgacgaggt cgacacgccc gactccacga tcgacctgcg gccgctccgc cccgacccgc
     1321 accccgacgc ggtgccgctc gacgccattg tcggctcggt cgcatgagca ccacgctggg
     1381 gagcgtgcgg gcggtggtcc cgagaccacc gcgcacatcg gagtcgcggc catgaccgcc
     1441 cggcgcgtgt ccggacgtcc ggcgcacgga agagacggaa catcgacaga ccggtacgga
     1501 gcatcgggct ccgcaccacg gactcctggg ggtccggagg agggaattga catgaaggcg
     1561 acgtcgaaca accgaaaggc ccgcggcgca ccccgccgac ggagcctcgt catcgcgttg
     1621 gcggtcgcgt tgatcgccgc ggtcgcaccg tcgctcaccg cgagcgtcgg cgccgcggac
     1681 ccggccaaac cggtgtcgat gcgactgctc gtcatcgccg cggacggcaa cgagaccgac
     1741 tacccggcca tcacgacctt cctcgacgag gtcggcgtcc cctacgacaa gatccttgcg
     1801 gcggacacgc cgctgaccgc gtcgatgctc tccgacggca cgactggcaa gtaccagggg
     1861 atcgtgctga cgaccggcaa cctgacctac ttcgacgccg gggccggcac ctggcagagc
     1921 gcgttcagcc ctgacgagtg ggcgacgctc cgccagtacc agaaggactt ccacgtccgc
     1981 tccgtgacca gctacacgtt cccggaggcg agctacggcc tgagctacgc cggctaccgc
     2041 aacaccctcg gatccgagct gccggccacc ctcaccgcag ccggccaggc ggtgtggccc
     2101 tatgtgaacg ctgccaaccc gatcgagctc accggcgcct gggtctatct gggttcgatc
     2161 atcgacccga gcgtgaccac gccgctcgtg aacgtcaccg tcgacgggac cacgtacccg
     2221 gtcgcctcgg tcacccgctt cgacgggtac gagaacctgg cggtcaccgc ggccaacaac
     2281 ccgaacctgc tccactcgat cctgctctcc accggatgga tcaactgggt cagcggaggg
     2341 aacttcctcg gcaaccgcag ctccagcatc gatgtccaga tcgacgacct gttcatcggg
     2401 accgaccagt gggacccggc gacgaacacc gctcacaccg agtacaccaa cgtgcccgcc
     2461 gacgtgacag cgatggtggc gtaccagaac gcccgtcggg ccaaccccgc cacgccgggg
     2521 ttcaaggtcg aatacgcctt caacggcgca ggaggcccga ccaacccgtc gctggtcaac
     2581 acggtgcgcg ccaaccgctc gcagttcggc ttcatcaacc acacgtggac ccacttcaac
     2641 ctggactgcg gcgactgccc caacccgacg gggcagatca ccacgaacac gacgcagatc
     2701 cgttcgcaga tcctgaccaa ccgcatgtac ggagcgttgc tcggtctgcc gggcaacacc
     2761 gacacgatgg tccagcccga catctcgggc atcaacacgc ctcccaaccc gatggcgcag
     2821 aaggccgccg gcggcgtggg catccgcttc tggatcggag acacgtcacg ggcggggcag
     2881 accaacccga cgttcaacac cggcttcgcc acagcgggcg actcccgggt gttcgtcgtg
     2941 ccccgtcgcc cgaccaacct cttcgtggcc gccaccacgc cggaccagtg gaccagcgtc
     3001 tacaaccact tctacgcccc tggcggcatc ctgtgcggca tgacgacctg cttcgaccgg
     3061 cctcagacct accaggagat cctcgaccac gagtccgact acctgctgcg ctacctgctc
     3121 cgaggtgacc tcgatccgtg gatgttccac gtcggcaaca cccgggccta ctccggcaac
     3181 cgcagcgtgc tgtccgatct cctcgacgag acgttctcga agtactcgtc gatggtcaac
     3241 acgccggtgc gcagtgtctc gttcaagcag gccggccagg ccatgcaggg ccgggccgcc
     3301 tacaacgctg cgggggtcac cgcgacggtg acgccgtgca ccagcatcac ggtcaaggcc
     3361 acaaaggccg cgaccgtgcc ggtgaacggg gtcgcctaca ccgcggcgaa ctcgacggtc
     3421 gaggcctacg gtggacggac catctcccag gttcgtctgg ccgctgggca gaccgtgacg
     3481 atcccgctgc ccgcatgctg accgagggct cgtcggtcgg ggtcccgacc gacgagccgg
     3541 ctgctgccgc cggcgctgct gacggtgcag ggcgctcatc gcggcccggt cggagatggc
     3601 tgctgctggc gggtgcggtg gtcctcgtcg cgttggttgc tggcgttgtc ctcatgtctc
     3661 gacgtggaga cgcggtcccg gacccggcgg cgacctcggt cgctcggtca gacgcgcctc
     3721 ggccggtggc gaccgttgcg tcggacgcgg ccccgtccac accgacggag gcgggcacac
     3781 cgtcgacgac ggtcgcttcg tcggcggcgg accctgcccc gctcacagca gaaccggggc
     3841 agccgggcac ggccgcagcg gcttcgatcc cttccgctcc ggaagcggtt cccgccccac
     3901 cactccgtcc cgttcccggg gccgaaccgt ctcgggccgc aatcgacgtc ccgcagtcac
     3961 ccggcccgcc ggccgccgat ccgacccaca gcggtcactg acaggagggt tccatcgtga
     4021 gcacaggatc gcccagatgg agggcggcgg tcaccggacg gcggcctgtg gtggccgttc
     4081 agggactcgg gttcgtgggc gcggccatgt cgatagcggt cgcctccgcg ctcgacgaca
     4141 ggggggagcc cgcgttcgac gtggtcggag tcgaccgcga cgacccggcg ggccgcgagc
     4201 gcatcgacgc gctcaactca ggtcggttcc cgttccccac gagtgacgac catctccgat
     4261 cggcgctcgc ggcggtacgc ggccgcggca acctcgtcgc agggtcggat cccgctgcat
     4321 acgcgcttgc cgacgtggtc gtgatcgatg tcccgctcga catcgactgg cgggccgagc
     4381 ctcctgagct gctgctcgag ggattcacct cggcgatccg caccgtcggt cgctacatcg
     4441 aacccggaac actggtcctc gtggagacga cggttccgcc cgggaccacc gaacgcgtcg
     4501 tggtgcctgc gctcgccgag gagcttgtcg cccggggtca ccgtcccgac gacgtattgg
     4561 tcgcccacag ctacgagcga gtcatgcccg gcgccgagta cctcgcctcg atcgtcaact
     4621 actggagggt gtacgccgga cggaccgagg ccgccgccaa ggccgccgag gcgttcctgt
     4681 cccaggtgat caacgtggcg gagtacccgt tgacccgttt gtcgaacacc accgcgtcgg
     4741 agacggcaaa ggtcctggag aacacgttcc gggccacgac catcgccctg atggacgagt
     4801 ggggacgctt cgccgagacc gtcgggatcg atctggtcga agtggtggat gccgtcagga
     4861 tgcgacccac ccacgccaac gtgcggacgc cgggcttcgg ggtgggcggg tactgcctga
     4921 ccaaggatcc gctcttcgcc cgtctcgcct ccgaggtcct gttcgactct ccggtcgagt
     4981 tcccgttctc cactgctgcg gtgcgcgtca acgaccaggc tcctgtgagg gcactcgacc
     5041 gactagagga gctgatggga cgccccgtca ccggacgtca tgtcctcctg ctcggggtgt
     5101 cgtaccgcca ggacgtcggc gacactcgtt actccccgtc cacgaccttc gttgaggccg
     5161 cggccgcgcg cggcgcacgg gtgagcgcgc atgacccgct ggtcgaccgc tgggaggagc
     5221 tggatagacc ggtgatgaca gacctcccac ccctcgacga cgtcgatgct gtggtcctcg
     5281 ccgtccccca cgacgactac cgacgtctcg acgtcaccac atggctcggc gagcaccgac
     5341 cggtcctgct cgacgcgttc gacgtctgga ccgacgacca acgacgggcg gcggaggctc
     5401 tcggctgcac ggtcggatcg atcggtcggg gcactccgcc gtgacgactc gtcgcggggc
     5461 catcgcatcg ttgaacggcg gatcaggagg aaggacatca tgagcggacg ggcactcatc
     5521 accggcgggg cgggcttcat cggagcccat ctcagccgac gactgctgga cgacggctgg
     5581 gaggtgaccc tcgtcgacaa cctgagtcgg ggcgtgcagg acacggccct ggcgtctctg
     5641 cgcgagcggc cgggattcgc actgcacgtc gccgatctgc gcgacccttc gaccctgggc
     5701 tcgatcccga ccgaccacac gcacgtcctg cacttcgccg ctcttctcgg cgtccagaac
     5761 gtgatcgatc ggccctcggc cgtgctccgt gagaacgtcg ctctgctcga gaccatgctc
     5821 gacgtggcgt cggcccagag ctctctcgag cgattcctgt tcccctcgac cagcgaggtg
     5881 tacgcgggaa cccagcagtt ccacgacctg ccgttcccga cgcctgaaga cactccgctc
     5941 accgtgtcgg cgctcgatcg cccgcgcacc agctacatgc tgtccaagat ctacggcgaa
     6001 gccctcgtcc accacagcg
//
LOCUS       DAOQOD010000182         5975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5221_length_5975_cov_11.690878, whole genome shotgun sequence.
ACCESSION   DAOQOD010000182 DAOQOD010000000
VERSION     DAOQOD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_5975_cov_11.690878"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLZ72_13700"
     CDS             complement(<1..431)
                     /locus_tag="PLZ72_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA37399.1"
                     /translation="MSDHAEADPTAAGDHPDRGFTHVALQVSDLDASLAFYERYADLV
                     PVHRRTSDDGTRVAWISDLTRPFVVVLIETSAAQARRHRLGGWNHLGVGVGSRAEVDR
                     RLADADAAGCRTDGPVDAGAPVGYFGIIADPDGHHLEIAHG"
     gene            547..1329
                     /locus_tag="PLZ72_13705"
     CDS             547..1329
                     /locus_tag="PLZ72_13705"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNA37400.1"
                     /translation="MDRQIASMQQLFGLDGRVAVVTGASSGLGDRFARVLHGAGATVV
                     VAARRAERLEALAADLGGRVVVVPADLTVAADRERLIEEAFDVAGRIDVLVNNAGYGI
                     PSPAESESIDRFEQTMDINVTAPFHLAQLAGRHMLAAGSGSIVNIASVLGLAASAPIK
                     EVSYCASKGAVVNLTRQLGCEWGRKGVRVNAIAPGWFPSEMTQAEMFDDEGGQSFIVR
                     NTPMGRAGELAELDGALLYLAGDASTYVTGHILAVDGGWLAR"
     gene            complement(1484..2422)
                     /locus_tag="PLZ72_13710"
     CDS             complement(1484..2422)
                     /locus_tag="PLZ72_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNA37401.1"
                     /translation="MGWNHLTDLARIAAMHDGVVSIDQAQHLGIPRRSVQRAERNGLV
                     VAMHRGVYRFTATPTSTTREIRAAVLAVGDDARASHESSLHLHGVNSIPRDRIVVSVP
                     PRRRADHPGIRVHRLIDQHAEHLCEVEGIPTTTLARAIVDVSSEFWRPRMNWLLDEVT
                     ITRRLVSDGAIARAYRQVNHRGRRRIAVLGELLDERSATGSTPRSTLEREMDELLDGS
                     VLPTPSREYPVPSMVPGAGFADRAWEDALLIVEIDGRTYHERRSAMRRDRERDRAAAR
                     MGWQTMRVLDEEVSDEPELVLDDIVTTYQRRLDQLR"
     assembly_gap    2556..2655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2812..3372)
                     /locus_tag="PLZ72_13715"
     CDS             complement(2812..3372)
                     /locus_tag="PLZ72_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37402.1"
                     /translation="MTTVSADIVLHPIGGEERTLEELTSLFHLVVVAIDPYEYESSWI
                     LATAGRILTEYEEADCRVAWLVTAPEEDAVAFLGPWAERMLTLCDPNRIAVNALGITE
                     IPALVHIGTDRSVVGKADGWDPEAWKPITDNLARVMSWSRSVYPKAGDPAPFRGSPVG
                     AATEPEPAAPDASDAASDTAAPATAD"
     gene            3445..4245
                     /locus_tag="PLZ72_13720"
     CDS             3445..4245
                     /locus_tag="PLZ72_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37403.1"
                     /translation="MHPIERLRFVARSQGAPADLLVEEAAGALGAFRDDPAGMVAACR
                     RIIDRQLTCGPLWWLCARILCAPDPMVEARAAVADLEADPTARLLASALPDDATVVVF
                     GVPELTLGALVRRGDVRPLVVDVEGAADDVVGYLERVDVRAEAVPARSVAVAVDRADL
                     VVVEASAAGPGAALVPSGARAGASVAARAGTPVWLALGTGRLLPERMWDALVSRWSAS
                     VDPFDAAEEELDLGHVERISGPDGPVEPAEAVRRIDCPVAPELFRLAG"
     gene            4257..4946
                     /locus_tag="PLZ72_13725"
     CDS             4257..4946
                     /locus_tag="PLZ72_13725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNA37404.1"
                     /translation="MGQLVEFPSNGSTAQGWFAPSATGSGPAVLVIQEWWGLVPHIQE
                     VVDRFAAEGFTALAPDLYHGETTTEPDEAGKLMMALNLEQAAKDLRGAIAYLKGSDAT
                     SSEGVGVIGFCMGGGLALVVACNEPQDVVACAPYYGLIPWEGAAPDYSRLSGPVHGHY
                     AENDGFFGPALAQALEQELVETHGKDAVLQVHPGVDHAFFNDHRPEVYDRTQADAAFA
                     ETVAFFRDAVR"
     gene            4957..>5975
                     /locus_tag="PLZ72_13730"
     CDS             4957..>5975
                     /locus_tag="PLZ72_13730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA37405.1"
                     /translation="MRVLIVTNDLPPQVGGIQYYVDQLARGLAATGDQVTLYGSTPRS
                     DTADGDWRRHDRDAPFEVVREPTSVLLPTPRVRRHAAELVRSLDAEVVVFGATVPLAF
                     MGPGLRRRTGVPYVCWTHGLEVSAVRMPGSGPLLRRIGRDAGAITYVSHWCRDLLAPA
                     FGAGVPPELLPPAVDPAGYHRGVDGSAVRARYGLADRPVVVCVSRLVERKGQDQLIRA
                     LPELRRRVPGTALLVVGGGPHREPLERLAAEVGVADDVVFTGIVEEAELPAHFAAGDV
                     FAMPCRERRGGFEVEAFGIVFIQAQAVGVPVVAGDIGGVPDAVGGVDPGDDPGGVLVD
                     GTDLAA"
BASE COUNT          713 a         2059 c         2221 g          882 t
ORIGIN      
        1 tggccgtggg cgatctcgag gtggtggccg tcggggtcgg cgatgatgcc gaagtacccg
       61 accggtgccc cggcgtcgac cgggccgtcg gtgcgacagc cggcggcgtc ggcgtcggcc
      121 aggcgccggt cgacctccgc ccgcgatccg accccgacac ccaggtggtt ccagcctccc
      181 agacggtggc ggcgggcctg ggcggcggag gtctcgatca gcacgaccac gaacggtcgg
      241 gtgaggtcgc tgatccatgc cacccgggtg ccgtcgtcgc tggtgcggcg gtggaccggg
      301 accaggtcgg cgtagcgctc gtagaacgcg aggctggcgt cgaggtccga cacctggagc
      361 gcgacgtggg tgaagccgcg gtcggggtgg tcgccggccg cggtcgggtc cgcctcggcg
      421 tggtcgctca tggtccgacg ctaccggcga gtccgccgtg ccccgcggag cggccggcgt
      481 ggtcggtgtg gccgcggcgc ggccgggccg gtcgggggca tcggaccgct cgggtaccgt
      541 gcggcggtgg accgacagat cgcatcgatg cagcagctct tcggactcga cggccgggtc
      601 gcggtggtga ccggcgcgtc atccggactc ggcgaccgct tcgcccgggt cctccacggc
      661 gccggcgcca ccgtcgtcgt cgccgcccga cgggccgaac ggctcgaggc gctggccgcg
      721 gacctgggcg gccgcgtcgt cgtcgtcccg gccgacctga ccgtcgccgc ggaccgggaa
      781 cgcctgatcg aggaggcgtt cgacgtggcc ggacggatcg acgtgctggt caacaacgcc
      841 ggctacggca tcccgtcgcc cgccgagtcg gagtcgatcg accgcttcga gcagacgatg
      901 gacatcaacg tgaccgcccc gttccacctg gcacagctgg ccggtcgcca catgctcgca
      961 gcgggatcgg gcagcatcgt gaacatcgcg tcggtgttgg ggttggcggc ctcggctccg
     1021 atcaaggagg tcagctactg cgcctccaag ggggcggtgg tcaacctgac ccgccagctc
     1081 ggctgcgagt ggggtcgcaa gggggtccgg gtgaacgcca tcgctccggg ctggttcccc
     1141 tcggagatga cccaggccga gatgttcgac gacgagggcg gtcagtcgtt catcgtgcgc
     1201 aacaccccga tgggtcgggc gggggagctg gccgagctcg acggagccct gctgtacctg
     1261 gccggcgacg cctcgaccta cgtgacgggc cacatcctcg ccgtcgacgg gggttggctc
     1321 gcccgctgag ccggcgccgc ccaccgaccg acccccgccg aacttgtagt ccccacgccg
     1381 cgtgggcacc cgtcagctac aaggtcgatc accgtccgtc ttgttgtagc ccgcgggtcg
     1441 ccggaccgac gggggactac aagttcgacg gtccgggagc gggtcagcgg agctggtcga
     1501 ggcgtcgctg gtaggtggtc acgatgtcgt cgagcaccag ctccggctcg tcggacacct
     1561 cctcgtcgag gacccgcatt gtctgccagc ccatccgggc cgctgcccgg tcacgttccc
     1621 tgtcgcggcg catggcggat cggcgctcgt ggtaggtgcg cccgtcgatc tcgacgatga
     1681 gcagggcgtc ctcccatgcc cggtccgcga acccggcccc cggaaccatc gacgggacgg
     1741 ggtactcgcg ggacggggtg ggcaggacgg atccgtcgag cagctcgtcc atctcccgct
     1801 cgagggtgct ccgcggcgtc gacccggtgg cggaacgctc gtcgagcagc tcccccagca
     1861 ccgcgatgcg tcgacgcccc cggtggttca cctggcggta cgcccgggcg atcgctccgt
     1921 cgctgaccag ccgtcgggtg atggtgacct cgtcgagcag ccagttcatc cggggtctcc
     1981 agaactccga ggagacgtcg acgatggccc gggccagggt cgtggtcggg atcccctcca
     2041 cctcgcagag gtgctcggcg tgctggtcga tcaggcggtg cacccggatg ccggggtggt
     2101 cggcccgacg tcggggtggc accgacacca cgatccggtc ccgagggatg ctgttcaccc
     2161 cgtggaggtg gaggctcgat tcgtggctgg cccgggcatc gtcgcccacc gccaggacgg
     2221 cggcccggat ctcgcgggtg gtcgaggtgg gcgtggcggt gaaccggtac acgccgcggt
     2281 gcatggccac gaccaggccg ttgcgctcgg ctctctgcac ggatcgacga gggatgccca
     2341 ggtgctgggc ctggtcgatc gacacgactc cgtcgtgcat ggcggcgatc cgtgccaggt
     2401 cggtgaggtg gttccatccc atgccgtcga caggaccacg ggtccggctc gggtggcaac
     2461 ggggaccgcg acccgttgac gccaccgtcg cgctgtgacc agcggcgttg ttgtagcgag
     2521 ggggtcgctg gggcgacggt gggctacaag ttcggnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnagcgg cgttgttgta gcgagggggt cgctggggcg acggtgggct
     2701 acaagttcgg ggggccgtgg cgttgttgta gcgagggggt cgctggggcg acggggggct
     2761 acaagttcgg cgggctacaa gttcgggggg ccgtggcgcc ggctcggccg gtcagtccgc
     2821 ggtggccggc gccgcggtgt cggacgccgc atcgctcgcg tccggagccg ccggctccgg
     2881 ctcggtggcc gcacccaccg gcgaacctcg gaacggtgcc gggtcgcccg ccttggggta
     2941 caccgagcgg ctccacgaca tcacgcgggc caggttgtcg gtgatcggct tccaggcttc
     3001 ggggtcccag ccgtcggcct tgccgacgac cgagcggtcg gtgccgatgt gcaccagcgc
     3061 ggggatctcg gtgatgccca gtgcgttgac ggcgatccgg ttcgggtcgc acagcgtgag
     3121 catgcgctcc gcccacggtc cgaggaacgc gacggcgtcc tcttcggggg cggtcaccag
     3181 ccacgccacc cggcagtccg cctcctcgta ctcggtcagg atgcggcccg ccgtggcgag
     3241 gatccaggag ctctcgtact cgtaggggtc gatcgccacg accaccaggt ggaagagcga
     3301 ggtgagctcc tccagggtgc gctcctcgcc gccgatgggg tgcagcacga tgtcagcgga
     3361 cacggtcgtc atcgggcccg aaaggtagcg cgaccgggcc atcgggcgac agatggggcc
     3421 cggccgctgc ctagagttcg gcgggtgcac cccatcgagc gactccgctt cgtggcccgc
     3481 tcccagggcg ccccggccga cctgctggtc gaggaggcgg ccggtgccct cggcgccttc
     3541 cgagacgacc cggccggcat ggtcgcggcc tgccgtcgga tcatcgaccg ccagctgacc
     3601 tgcgggccgc tgtggtggct gtgcgcacgc atcctctgcg ctcccgatcc gatggtcgag
     3661 gcccgcgcag cggtggccga cttggaggcg gacccgaccg cccgtctgct ggcgagcgcg
     3721 ctgcccgacg acgccaccgt cgtcgtcttc ggcgtgcccg agctgaccct gggggcgctc
     3781 gtccgtcggg gcgacgtccg cccgctggtg gtcgacgtcg agggcgcggc cgacgacgtc
     3841 gtcgggtacc tggaacgggt cgacgtccgt gccgaggcag tcccggcccg atcggtcgcc
     3901 gtcgccgtcg accgggccga cctggtcgtc gtcgaggcgt cggccgctgg tcccggagct
     3961 gcgctggtgc cctcgggcgc ccgggccggc gcatcggtgg cggcacgcgc cggcacgccg
     4021 gtgtggctcg ccctcggcac cggtcggctg ctgcccgaac gaatgtggga cgcgctggtg
     4081 tcgcggtggt cggcctcggt cgaccccttc gacgccgccg aggaggagct cgatctcggc
     4141 cacgtcgagc ggatctcggg tccggacgga ccggtcgagc ccgccgaggc cgtccgacgg
     4201 atcgactgcc cggtggcccc ggagctgttc cgcctggccg ggtagggttc gggcccatgg
     4261 gacagttggt ggaattcccc tcgaacggct cgacggcaca ggggtggttc gccccctcgg
     4321 ccaccggatc gggccccgcg gttctcgtga tccaggagtg gtggggtctg gtgccccaca
     4381 tccaggaggt ggtcgaccgc ttcgcggcgg agggcttcac cgctctggcc ccggacctct
     4441 accacggcga gacgaccacc gagcccgacg aggccggcaa gctcatgatg gcgctcaacc
     4501 tggagcaggc ggccaaggac ctgcgcggcg ccatcgccta tctcaagggc agcgacgcca
     4561 cctcgagcga gggcgtgggc gtcatcggct tctgtatggg gggcggcctg gcgttggtcg
     4621 tcgcctgcaa cgaaccccag gacgtcgtcg cgtgcgcgcc ctactacggg ctgatcccct
     4681 gggagggcgc cgcgccggac tactcccggc tgtcgggtcc ggtccacggt cactacgccg
     4741 agaacgacgg gttcttcgga cctgctctgg cccaggcgtt ggagcaggag ctggtcgaga
     4801 cccacggcaa ggacgccgtg ctgcaggtcc accccggcgt cgaccacgcc ttcttcaacg
     4861 accaccgccc cgaggtgtac gaccggaccc aggccgacgc cgcgttcgcg gagaccgtcg
     4921 cgttcttccg cgacgcggtg cgctgacccg gcgcgcgtgc gcgtcctgat cgtcaccaac
     4981 gacctgccgc cccaggtcgg gggcatccag tactacgtcg accagctggc ccgcggtctg
     5041 gccgcgacgg gcgaccaggt caccctctac ggctccactc cccgcagcga caccgcggac
     5101 ggcgactggc gccgccacga ccgtgacgcc cccttcgagg tggtgcggga gcccacctcg
     5161 gtgctgctgc ccaccccacg ggtccgtcgt cacgccgccg agctggtgcg ttcgctcgac
     5221 gccgaggtgg tcgtgttcgg ggcgacggtc ccgctggcgt tcatgggccc cggcctgcgt
     5281 cgccgcaccg gcgtcccgta cgtgtgctgg acccacggcc tcgaggtctc ggcggtgcgg
     5341 atgccgggca gcggcccgct gcttcgccgg atcggacgcg atgccggtgc gatcacctac
     5401 gtgtcgcact ggtgtcggga cctgctcgca ccagcgttcg gcgccggggt tcccccggag
     5461 ctgctcccac ccgcggtcga ccccgccggg taccaccgcg gcgtcgacgg atccgcggtc
     5521 cgggcccgat acgggctggc cgaccggccg gtggtggtgt gcgtgtcgcg gctggtcgag
     5581 cgcaagggcc aggaccagct gatccgggcc ctgcccgagc ttcgtcgccg ggtccccggc
     5641 accgcgctgc tcgtcgtcgg gggtggtccg caccgggagc cgctcgaacg cctcgctgcc
     5701 gaggtcgggg tggccgacga cgtcgtgttc accgggatcg tcgaagaggc cgaactgccc
     5761 gcccacttcg ccgccggcga cgtgttcgcc atgccgtgcc gggagcgtcg gggcggcttc
     5821 gaggtcgagg cgttcgggat cgtgttcatc caggcccagg cggtcggtgt cccggtggtc
     5881 gcgggcgaca tcgggggtgt gcccgacgcg gtcggcggcg tcgacccggg cgacgacccc
     5941 ggcggggtgc tcgtcgacgg cacggacctg gccgc
//
LOCUS       DAOQOD010000183         5967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5231_length_5967_cov_9.415765, whole genome shotgun sequence.
ACCESSION   DAOQOD010000183 DAOQOD010000000
VERSION     DAOQOD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5231_length_5967_cov_9.415765"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1879)
                     /locus_tag="PLZ72_13735"
     CDS             complement(104..1879)
                     /locus_tag="PLZ72_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNA37406.1"
                     /translation="MNTSFADLGVPAPIVASLAERGIDAPFPIQALTLADTLAGRDVS
                     GKAPTGSGKTLAFGIPLVVGCPTAERRRPRRLVLVPTRELATQVADELSKLAGRKGPR
                     VAAFFGGVPIPRNIKALERGVDIAVACPGRLTDLIDQRLVRLDDVDVVVLDEADRMAD
                     MGFLPQVRKLLDKCRDDRQTVLFSATLDGDIDVLVRNYQHDPVLHELEVPEEELPDIA
                     HHFWAVDRTERVATAAQVVDRMGPTVVFCRTKRGAERVAKQLTNAGVAAAAIHGDRSQ
                     NQRDRALEDFRAGRAQALVATDVAARGIHVDDVAAVIHFDPPEDPKDYVHRSGRTARA
                     GAAGVVVCLVIPEKRSAVRGLQRSLDLPSGLLDADPTSLGEPAVRPARIAPPSNRRSK
                     TEARDASSGRVGSDAATPDRTSRSNRERNGEPRRNGRDGQDGRDGQDGRKPGQARNDR
                     SRGRNERSTSDRSRTDRPRDDRRRSDTRTGEDVRADDRRSRSHTDRDRTDRDRTDRDA
                     AERPDSPRRRRHRRRHEQNPRGTGGSGRSTGSRAGGRSKTTGNNKTTGNDSRAGNGRP
                     AGNDSRSATGKGSRTSRQSSRGPRR"
     gene            complement(2120..3142)
                     /locus_tag="PLZ72_13740"
     CDS             complement(2120..3142)
                     /locus_tag="PLZ72_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA37407.1"
                     /translation="MTPDRDAPSTPTRPDIEVLRPDPPPQRLLVRWRRAGRAEGRDAE
                     WHPARAAILAAIVVALAWWLPGAAVFLGILVALILVHELGHLVVARRCGMAPTEYFLG
                     FGPTVWSRTSRRGLRYGIKAVVLGGYVKVPGMGPSEEVEASLEPFTYRAASRPRRLAV
                     ILAGVAVNFAVALALFWSYAMWSPQIDVGPTRAATGSVSLMWEVSHDTVTSLGELVAG
                     AADYSKSVADGEVPEQRMVSAVGGAQLTDGLLDQHPSRLLLLAGLFSTSIAVFNLLPL
                     LPLDGGHAALIVAEGAIARVRRRPRYRLDPNRFRVAAAAVVVVLLALGATSMYIDVLH
                     PLSAVG"
     gene            complement(3425..4264)
                     /locus_tag="PLZ72_13745"
     CDS             complement(3425..4264)
                     /locus_tag="PLZ72_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37408.1"
                     /translation="MAEHLDRIVDNNAALYRLLDEGFAADGRYHRTMGNHDDVYEDEQ
                     MVQHLRRHLPGTEVVDTVLLTAPERVTSRPGATGPRWGDREGLAAVDAVVAHGHLTDA
                     WNGPGTALLGRIITWTATAFDDLPGLNGATNPLPDEEAVGRLLTGRARNRLITLDPRI
                     GGNRRFDSLDEERLFSHLAAVATARGEAIGAVRPDGSVGWPWLLFGHTHLPMLSPLDA
                     TGSPVRYANSGTSLLDHALTSIEWDPARREPRLVVWTDEPDGPRRRELIADGAHLAVL
                     DDA"
     gene            complement(4264..4890)
                     /locus_tag="PLZ72_13750"
     CDS             complement(4264..4890)
                     /locus_tag="PLZ72_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37409.1"
                     /translation="MAVPLPPPLNRLRAQVGAAIPSWEEVLERAGRVADEADHLNRVG
                     ARPEDGPPPPGMTASAWAVLKASATVMGGPVSLPAMVLAGRRAQRTALARIDRFDEHL
                     QAVAEGRRSPLPAPRRSRLSSDRRIVITSDLHRCVPGRLDWPARQRTKDLSLRVMSHY
                     AEQGWDLIENGDVEDFWLVGGSTWGAMYDVGRIAGGVGVGPPMPRGAA"
     gene            5040..5825
                     /locus_tag="PLZ72_13755"
     CDS             5040..5825
                     /locus_tag="PLZ72_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNA37410.1"
                     /translation="MTINERAPEGDRSYAAVADRLTMPLVEAMETQRAVRRILPDPVD
                     DDIVLRCIELGLKAPTGSNGQNWQFVVVKDREVLATFARRYRQAWSLYGGLGERVVGN
                     DESMRKILAAVAWQVEHFTEAPVVVVPCLEGAQRFAPVPALAASSWYGSIYPSVQNLL
                     LAARAMGLGASLITLPLWSVTLSRRTLGLPMTVQPVCMVPLGWPRGRYGPTTRKPVGD
                     VVHLDRFGTRPWRGQGVGRPQERSVAAADDPTPAERDATEVRS"
BASE COUNT          754 a         2074 c         2305 g          834 t
ORIGIN      
        1 ggcggcttcg gccacaccgg ccgctgactg cagcagccgt gcgggttctg gctgcagttc
       61 aggccgcccc ggcagcctga actgcagcca gaactgcttc gggtcagcga cgcgggcctc
      121 gggagctctg acgtgacgtc cgtgacccct tgccggtcgc ggagcgactg tcgttcccgg
      181 cgggtcggcc gttcccggcg cgactgtcgt tgccggtggt cttgttgttg ccggtggtct
      241 tgctcctgcc tccggcgcgc gagccggtgg accgaccgga tccgccggtg ccgcgggggt
      301 tctgctcgtg gcggcggcgg tggcgtcgac gccggggcga gtcgggccgt tccgccgcgt
      361 cgcggtcggt gcgatcgcgg tcggtgcgat cgcggtcggt gtggctgcgg ctcctgcggt
      421 cgtcggccct gacgtcctcg ccggtgcggg tgtcgctgcg ccgccggtcg tcgcggggcc
      481 ggtcggtccg gcttcgatcg ctggtgctcc gctcgttgcg gccacgggag cggtcgttgc
      541 gggcctgccc gggcttgcgc ccgtcctgcc cgtcccgacc gtcctgcccg tcccgaccgt
      601 tacggcgggg ctccccgttg cgctcacggt tcgatcggga ggtgcggtcc ggtgtcgcgg
      661 cgtcgctccc gacccggccg gacgacgcgt cgcgggcctc ggtcttggaa cgccggttgg
      721 acggaggcgc gatgcgagct ggtcggacgg ccggctcgcc gagagaggtg gggtccgcgt
      781 cgagcaggcc gctcggcagg tccaggctgc gctgcagccc gcggaccgcg gaccgcttct
      841 cggggatgac caggcacacg acgaccccgg ccgcgccggc ccgggcggtg cgcccggagc
      901 ggtgcacgta gtccttgggg tcctcgggcg ggtcgaagtg gatgaccgcg gccacgtcgt
      961 cgacgtggat tccccgggcg gcgacgtcgg tggccaccag cgcctgggcc cgaccggccc
     1021 ggaagtcctc gagcgctcgg tcgcgctggt tctgggaacg atcgccgtgg atcgcggctg
     1081 cggccacgcc ggcgttggtg agctgcttgg cgacgcgctc ggcgccgcgc ttggtccggc
     1141 agaacacgac cgtcggcccc atgcggtcca cgacctgtgc tgcggtggcg acccgctcgg
     1201 tgcggtcgac ggcccagaag tggtgggcga tgtcggggag ctcctcttcg gggacctcga
     1261 gctcgtgcag gacggggtcg tgctggtagt tgcggacgag cacgtcgatg tcgccgtcga
     1321 gcgtggcgga gaagagcacg gtctggcggt cgtcacggca cttgtcgagc agcttgcgga
     1381 cctgcggcag gaagcccatg tcggccatgc ggtcggcctc gtcgagcacg accacgtcga
     1441 cgtcgtcgag gcggaccagg cgttggtcga tcaggtcggt caaccggccg ggacacgcga
     1501 cggcgatgtc gacgccccgc tcgagcgcct tgatgttgcg ggggatgggg acgccaccga
     1561 agaacgccgc gacccgcggg cccttccgtc cggcgagctt gctgagctcg tcggccacct
     1621 gggtggcgag ctcacgggtc ggtacgagga ccagccgacg gggtcggcga cgttcggcgg
     1681 tggggcagcc gacgacgagg gggatgccga aggccagggt cttgccggac ccggtcgggg
     1741 ccttgcccga gacgtcgcgg ccggcgagcg tgtcggccag ggtcagggcc tggatgggga
     1801 agggggcgtc gatgccgcgc tcggccagcg aggcgacgat cggcgcgggc acgccgaggt
     1861 cggcgaagga ggtgttcact gtcattcctg ttctggttgt tgccgcatcg ccgtgacggc
     1921 gtgggcggcg ggcgcgacga ccgggatcgg gcgacgtgcg atggggggga tgtctcaggt
     1981 ggttctcgcc ggaatgcagt tggcgtcacg acacctgcga ctggcgaacg cccacggtgc
     2041 ccgaaggctc gacgtgatgc gtgaaggaca ccgtagcagg ctcccggccg gatccgcccg
     2101 tccgggggtg gcaggcggct cagccgacgg cgctgagagg gtgcagcacg tcgatgtaca
     2161 tgctggtggc gccgagggcc agcagcacca cgaccaccgc ggcggccgcc acccggaacc
     2221 ggttcgggtc cagccggtac cggggccgtc ggcgtacccg ggcgatggct ccttcggcca
     2281 cgatcagcgc ggcgtgaccg ccgtcgagcg gcagcagcgg cagcaggttg aacaccgcga
     2341 tcgacgtgga gaacaggccg gcgagcagca gcagtcggct cgggtgctgg tcgagcagcc
     2401 cgtcggtgag ctgcgccccg ccgaccgcgg agaccatgcg ctgctcgggg acctctccgt
     2461 cggcgaccga cttcgagtag tcagcggcgc cggcgacgag ctcgcccagg ctggtgacgg
     2521 tgtcgtgcga gacctcccac atcagcgaca cggatccggt cgcggctcgg gtggggccga
     2581 cgtcgatctg cggtgaccac atcgcgtagc tccagaacag ggccagcgcg acggcgaagt
     2641 tgacggccac cccggccagg atgaccgcca gccgacgagg acgagacgcc gcgcggtagg
     2701 tgaacggctc gagtgacgcc tcgacctctt cggacggacc catgcccggc accttgacgt
     2761 agccgcccaa cacgacggcc ttgatgccgt agcgcaggcc gcgacgcgac gtccggctcc
     2821 acacggtggg accgaagccc aggaagtact cggtgggcgc catcccgcag cgtcgtgcga
     2881 cgaccaggtg accgagctcg tgcaccagga tcagcgcgac gaggatcccc aggaacaccg
     2941 cggcgcccgg cagccaccag gccagggcga cgacgatggc ggccaggatc gcggctcggg
     3001 cggggtgcca ttcggcgtcg cggccttcgg cgcgtccggc gcgacgccag cgcaccagga
     3061 gtcgttgcgg ggggggatcc gggcggagga cctcgatgtc gggtcgggtc ggggtcgagg
     3121 gtgcgtcacg gtccggagtc acctccggtc gctgcgtccc ggtcccggtg gtcccggccc
     3181 ggaccccggc gtgaggccgg gtgggaccgg ggacgacggg accacccgcc tgatggtcgc
     3241 tccccagctg catcgatgcg caacctatcc accgcggagc tacggcgaaa gcgcgaccag
     3301 cgttcttgta ggtggcggtc gctggggtga cctgtgggct acaagttcgg tgggccctgc
     3361 cgttgttgta ggtggcggtc gctggggtga cctgtgggct acaagttcgg cggtggcgcc
     3421 ggcgtcaggc gtcgtcgagc acggcgaggt gcgcgccgtc ggcgatcagc tcgcggcggc
     3481 gggggccgtc gggttcgtcg gtccacacca ccagccgggg ctcgcgacgc gccgggtccc
     3541 actcgatcga cgtcagcgcg tggtcgagca ggctcgtgcc cgagttggcg tagcggaccg
     3601 gcgacccggt cgcgtcgagc ggcgacagca tcggcaggtg cgtgtggccg aacagcagcc
     3661 acggccaacc caccgatccg tcgggtcgca ccgctccgat cgcctcgccg cgcgccgtgg
     3721 ccaccgccgc cagatgcgag aacagtcgct cctcgtccag cgagtcgaac cggcggttgc
     3781 cgccgatgcg ggggtcgagc gtgatgaggc ggttccgggc ccgtccggtg agcagccgtc
     3841 cgaccgcctc ctcgtcgggc agcgggttgg tcgcgccgtt caggccgggg aggtcgtcga
     3901 aggcggtcgc cgtccacgtg atgatgcggc cgaggagcgc ggtgcccgga ccgttccagg
     3961 cgtcggtcag gtggccgtgg gcgacgacgg cgtcgacggc ggccagcccc tcccggtcgc
     4021 cccaccgcgg gccggtcgcc ccgggacgcg acgtgacccg ctccggcgcg gtcagcagca
     4081 cggtgtcgac gacctcggtc cccgggaggt ggcgccgcag gtgctgcacc atctgctcgt
     4141 cctcgtagac gtcgtcgtgg ttgcccatcg tccggtggta gcgaccgtcg gccgcgaagc
     4201 cctcgtcgag caaccggtac agcgccgcgt tgttgtcgac gatccggtcg aggtgctcgg
     4261 ccatcaggcc gcgccgcggg gcatcggcgg cccgaccccc acaccaccgg cgatgcgccc
     4321 gacgtcgtac atcgcccccc acgtggatcc gcccaccaac cagaagtcct cgacgtcgcc
     4381 gttctcgatc aggtcccagc cctgctccgc gtagtgcgac atcacccgca ggctcaggtc
     4441 cttggtccgt tgacgggccg gccagtccag tcgcccgggg acgcagcggt gcaggtcgga
     4501 ggtgatcacg atccgccggt ccgagctcag tcgcgaccgc cgcggcgccg gcagcgggct
     4561 gcgacgaccc tcggcgacgg cctggaggtg ctcgtcgaag cggtcgatcc gcgccagggc
     4621 cgtccgctgg gcccgtcgcc ccgccagcac catcgccggc aggctcaccg gtccgcccat
     4681 gaccgtggcc gacgccttga gcaccgccca cgccgacgcg gtcatccccg gaggcggagg
     4741 gccgtcctca ggtcgggcgc cgacccggtt gaggtggtcg gcctcgtccg cgacccgccc
     4801 cgctcgttcc aggacctcct cccaggacgg gatggcggcg ccgacctgag ctcgcagccg
     4861 gttcagcgga gggggcaggg ggacggccaa gggccaagct tgccaccggc gccgccccgg
     4921 gcggacagca cggagcggtc cggtgcgccg gtaccgccgg tgggaccgac cgcgtatcgt
     4981 tcgcgcccga cgcgccgctc cgggtggacc ggccgggcac gaggatcggg gggggacaga
     5041 tgacgatcaa cgaacgggca ccggaggggg accgctccta cgccgcggtg gcggaccggc
     5101 tgaccatgcc cctggtcgag gcgatggaga cccagcgggc ggtgcgacgc atcctgcccg
     5161 accccgtcga cgacgacatc gtgctgcgct gcatcgagct cggcctcaag gccccgaccg
     5221 gatccaacgg ccagaactgg cagttcgtgg tcgtcaagga ccgggaggtg ctcgcgacct
     5281 tcgcccgccg gtaccgccag gcgtggtcgc tctacggcgg gctcggcgaa cgggtcgtcg
     5341 gcaacgacga gtcgatgcgc aagatcctgg ccgcggtggc ctggcaggtc gagcacttca
     5401 ccgaggcgcc ggtggtggtc gtgccgtgcc tggagggggc gcagcgcttc gctccggtcc
     5461 cggcgctggc ggccagcagc tggtacggct cgatctaccc cagcgtgcag aacctgctgc
     5521 tggcggctcg ggcgatgggg ttgggcgcgt cgctcatcac gctgccgctg tggagcgtga
     5581 ccctgtcgcg tcgcacgctg ggcctgccga tgacggtcca gccggtgtgc atggtcccgc
     5641 tcggctggcc ccggggccgc tacgggccga ccacccgcaa gccggtcggc gacgtcgtgc
     5701 acctggaccg cttcggcacc cgaccgtggc ggggtcaggg cgtgggccga ccccaggaac
     5761 gatccgtcgc cgccgcggac gacccgaccc cggcggagag ggacgcgacc gaggtccggt
     5821 catgaccgcg ggcaccgacg gcgggaaccc gaccgggcgg tccatgccgt cggcgatcga
     5881 cgccgcgacc gcggtccgcg acggctcgct ggccgccctc gagctggtcg aggactgcct
     5941 ggcccgcatc gcggcgggcg acgaccg
//
LOCUS       DAOQOD010000184         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5345_length_5886_cov_10.853713, whole genome shotgun sequence.
ACCESSION   DAOQOD010000184 DAOQOD010000000
VERSION     DAOQOD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5345_length_5886_cov_10.853713"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="PLZ72_13760"
     CDS             complement(<1..1578)
                     /locus_tag="PLZ72_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNA37411.1"
                     /translation="MLRRADVALRHQVTLADLADRWVGLHGDRLMVTEEGPAPVASGS
                     RRDGGRATGRAGDAGEIRELTYGAAATRVARWSAAIAARTGPGDTVVLFTPNGIDQFL
                     LCLAVSRAGRIPVPVNPQMRPREIDHVIVDSGATLVVRGAAELSSGPGSRSAPEPPDR
                     PELDDVAALFYTSGTTGTPKGVELTHRALIGGLAPMAALPTWLRHDELVLSLPIAHIM
                     GFISVLGPTLAGIPMYFLWHFRADRVLDVIEQRRSSAFMGVPATYRLLDEAGAATRDL
                     TCVRAWMTGADVMPGPLARKFKSYGASATLPVIGPVGEAAFFEGYGMVETGGGVVAKL
                     SPPLLPVGLGDSLGFTLPGWKVRVADLDDQPVAAGAVGELQIKGPGVLKGYHGDAAAS
                     AGVLTDDGWLRTGDLVRSGPFGLLSFQGRSKNVIKAGGYSVYAVEVEADLEEHPDVVE
                     AGVVGIDDAKLGQVPVAAVRLRDGATVTPDELVSWSKGRMARYKAPRRIVVVDELPRT
                     GTRKIRRDHLVPLFDADS"
     assembly_gap    1680..1779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1780..>3164)
                     /locus_tag="PLZ72_13765"
     CDS             complement(<1780..>3164)
                     /locus_tag="PLZ72_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNA37412.1"
                     /translation="ADMRNLLEFENTFPDATTVVLEQNYRSTQTILDAANAVIAHNTA
                     RKPKDLWTDEGPGERIVRFQGDDEAEESQWIAHQISSLHDRGDVLWSDFAVFYRTNAQ
                     SRVLEEQFLRSGIPYRVVGGLRFYDRREIKDAMAYLKAVVNPTDEVSVKRVLNTPKRG
                     VGDQTVGRLDAYARAHEIPFLDALRRWDDAGASARAARGIEAFTTLLDELSLELDKGP
                     GHVLEQILERSGYAAELEAENTLEAEGRQENLAELVGMAREYATVDEFLEQVSLVADT
                     DQLPDSTDPTDTGVVLMTLHAAKGLEFPNVFLIGLEEGVFPHMRSLTEPAELEEERRL
                     AYVGITRARERLFLSHAWCRTLHGSTQYNPPSRFLDEIPAELVVEAEASRAGRSGRSS
                     QRSGGWESTSWTPSGRDRQIERATRPAPPSPTGAHELGFRVGDDVEHTKWGEGVILHM
                     EGQGDKTEAIVR"
     assembly_gap    3165..3264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3265..3980)
                     /locus_tag="PLZ72_13770"
     CDS             complement(<3265..3980)
                     /locus_tag="PLZ72_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNA37413.1"
                     /translation="MDADDLLEGLNPAQYEAVTHGDGPLLVVAGAGSGKTRVLTHRIA
                     HLIKVHGVSPFEILAITFTNKAAGEMKERIGRLVGPVAEKMWVSTFHSACVRILRRDA
                     TLLGYPSAFTIYDQADAVRLTGYVLRDLNIDPKRFPPRSVHATISAAKNDGLSAEDYL
                     ERASVIHERRIGEVFEEYQARLRKAGAMDFDDLLGVTVELLRTEPQVLEHYRNRFGNV
                     LVDEYQDTNPVQNDLVMLLG"
     gene            complement(4072..5004)
                     /locus_tag="PLZ72_13775"
     CDS             complement(4072..5004)
                     /locus_tag="PLZ72_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA37414.1"
                     /translation="MARPFRFTVQASHMARPEDLVPLARRAEDVGVSVLTVADHLDDQ
                     LAPIAALMAAADATTTLRVGTLVFANDYRHPALLAKEAATVDRLSGGRLEFGLGAGWM
                     TTDYTATGIPLDPPSVRIARLEEALEVIRALWSGEPVEHHGCFYDISGLVGTPSPAQQ
                     PHPPIVIGGGGRKVLEVAGRHADVINLNPSLPAGVIDDRAGPSATPEATEQKIGWIRA
                     AAGDRFDGIELGARIHLAIVTNDRQEMFDALAGGFGMTAEQAAGSPHALCGTLDQIAD
                     DLEERRERFGISAIGLSASSLDDLAPLISRLAGT"
     gene            5093..>5886
                     /locus_tag="PLZ72_13780"
     CDS             5093..>5886
                     /locus_tag="PLZ72_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNA37415.1"
                     /translation="MTDSTAGDAPADNTPAIDGFRAETFTFASRARTIYTGGDGPAVI
                     VMAEMPGITPAVIAFARRVIDAGFTVVMPVLFGEPGRDISLPYMATSLAPACVSKEFR
                     AFALRTTPPAIDWLRALARHCHERHGGPGVGAVGMCFTGGFALAMATEPEMLAPVLSQ
                     PSLPIGGAKRKADLGLSDADLMAVRDRVDSDDICVLGLRFTGDKLAPPERFASLRREL
                     GENFIGVEIDSSPGNPWGHRRLAHSVLAEDLVDEPGQPTHEALEQVL"
BASE COUNT          789 a         1994 c         2084 g          819 t
ORIGIN      
        1 cgagtcggcg tcgaacaacg ggacgaggtg gtcgcgacgg atcttgcggg tgccggtgcg
       61 gggcagctcg tcgaccacca cgatccggcg cggcgccttg tagcgggcca tccgaccctt
      121 cgaccagctc accagctcgt ccggtgtgac cgtcgctccg tcgcgcaggc gcaccgcggc
      181 gaccggcacc tggcccagct tggcgtcgtc gatcccgacc acccccgcct cgaccacgtc
      241 ggggtgctcc tccaggtcgg cctcgacctc gaccgcgtac accgagtagc cgccggcctt
      301 gatcacgttc ttggaacgcc cctggaacga cagcagcccg aacggcccgc tgcgcaccag
      361 gtcgccggtg cgcaaccagc cgtcgtcggt gagcacaccg gcggaggcgg ccgcgtcgcc
      421 gtggtagccc ttgagaacac ccggaccctt gatctgcagc tcacccaccg cgcccgccgc
      481 gaccggctgg tcgtccagat cggcgacccg gaccttccag cccgggaggg tgaagccgag
      541 cgagtcgccg agtccgaccg gcagcaacgg cggcgacagc ttggccacca ccccgccgcc
      601 ggtctcgacc atgccgtagc cctcgaagaa cgcggcctca cccaccggtc cgatcaccgg
      661 cagcgtggcc gatgcgccgt aggacttgaa cttccgggcc agcggaccgg gcatcacgtc
      721 cgcaccggtc atccaggccc gcacgcaggt gagatcgcgg gtggctgcgc ccgcctcgtc
      781 gagcaggcgg tacgtggccg gcacccccat gaacgccgag gatcgacgct gctcgatgac
      841 gtcgagcacc cggtcggcac ggaagtgcca caggaagtac atcggaatgc cggccaacgt
      901 cgggccgagc acagagatga agcccatgat gtgcgcgatc ggcaggctca gcaccagctc
      961 gtcgtggcgc aaccacgtgg gcagcgcggc catcggtgcg agtccgccga tcaacgcccg
     1021 gtgggtgagc tcgacgccct tgggggtgcc cgtcgtgccc gacgtgtaga agagcgcggc
     1081 gacatcgtcg agctcggggc ggtccggcgg ctcgggagcg gagcgcgacc cgggcccgga
     1141 gctcaactcg gcggccccac gaacgaccag ggtggcgccg ctgtcgacga tcacgtggtc
     1201 gatctcccgc ggccgcatct gcgggttgac cggcaccggg atccgaccgg cgcggctcac
     1261 cgccaggcac aacaggaact ggtcgatccc gttcggggtg aacagcacga ccgtgtcgcc
     1321 cgggccggtg cgcgccgcga tcgccgcgga ccagcgggcc acacgcgtgg cggcggcgcc
     1381 gtaggtcagc tcacggatct cacccgcgtc gcctgcacga ccggtggctc gcccgccgtc
     1441 acgccgcgaa cctgaggcga cgggggccgg gccctcctcg gtcaccatga gccggtcgcc
     1501 gtgcagtccg acccaccggt cggccagatc cgcgagcgtc acctggtgac gcagggcgac
     1561 gtcggcccgt cgcagcatcc gcaacatcat cccccgagca tgtcacgggc cggctcggac
     1621 cccgcgatcc accctccccc ggcattctca tgaccgttcg cgccactggt ggcgcgaacn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcacgatggc ctcggtcttg
     1801 tcgccctggc cctccatgtg caggatcacg ccttcgcccc acttggtgtg ctcgacgtcg
     1861 tcgccgaccc ggaagccgag ctcgtgggcg ccggtcggcg acggcggcgc ggggcgcgtg
     1921 gcgcgctcga tctggcggtc ccgacccgac ggcgtccacg acgtcgattc ccagccgccc
     1981 gatcgctgcg aggaccgccc cgaccggccc gcccggctgg cctcggcctc gaccaccagc
     2041 tcggcgggga tctcgtcgag gaaacggctc ggcgggttgt actgcgtgga gccgtgcagg
     2101 gtgcgacacc aggcgtgcga caggaacagc cgttcgcggg cgcgggtgat gccgacgtag
     2161 gccaggcggc gctcctcttc cagctcggcc ggctcggtga gcgaccgcat gtgcgggaac
     2221 acgccctctt ccaggccgat gaggaacacg ttggggaact cgaggccctt ggcggcgtgc
     2281 agggtcatca gcacgacgcc ggtgtcggtc gggtcggtcg agtcgggcag ctggtcggtg
     2341 tcggccacca gcgagacctg ctcgaggaac tcgtcgaccg tggcgtactc gcgggccatg
     2401 ccgacgagct cggccaggtt ctcctgacga ccctcggcct cgagcgtgtt ctcggcctcg
     2461 agctccgcgg cgtagcccga acgctccagg atctgctcga ggacgtgccc gggacccttg
     2521 tcgagctcga gggagagctc gtcgaggagg gtggtgaacg cctcgatgcc ccgggcggcg
     2581 cgggcgctgg cgccggcgtc gtcccagcgg cgcagcgcgt cgaggaacgg gatctcatgc
     2641 gcccgggcgt aggcgtcgag gcggccgacg gtctggtcgc cgaccccgcg cttgggcgtg
     2701 ttgagcaccc gcttgacgct gacctcgtcg gtggggttca ccaccgcctt gaggtaggcc
     2761 atggcgtcct tgatctcacg ccggtcgtag aagcgcaggc cgccgacgac ccggtacggg
     2821 atgccggagc gcaggaactg ctcctcgagc acgcggctct gggcgttggt gcggtagaag
     2881 acggcgaagt cggaccacag cacgtcgccg cggtcgtgca gcgagctgat ctggtgcgcg
     2941 atccactgcg actcctcggc ctcgtcgtcg ccctggaagc ggacgatgcg ttcgccgggg
     3001 ccctcgtcgg tccacaggtc cttgggcttg cgggcggtgt tgtgggcgat gacggcgttg
     3061 gcggcgtcga ggatggtctg ggtggagcgg tagttctgct ccaacacgac ggtggtggcg
     3121 tcggggaagg tgttctcgaa ctcgaggagg ttgcgcatgt ccgcnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnntcgccg agcagcatga ccaggtcgtt ctgcaccggg
     3301 ttggtgtcct ggtactcgtc gacgagcacg ttgccgaacc ggttgcggta gtgctcgagc
     3361 acctgaggtt cggtgcgcag cagctcgacg gtgacgccga ggaggtcgtc gaagtccatg
     3421 gcgccggcct tgcgcagacg ggcctggtac tcctcgaaca cctcgccgat gcgccgctcg
     3481 tggatgaccg aggcgcgctc caggtagtcc tcggcggaca ggccgtcgtt cttggccgcc
     3541 gagatggtgg cgtgcaccga ccggggcggg aaccgcttgg ggtcgatgtt gaggtctcgc
     3601 agcacgtagc cggtgaggcg cacggcgtcg gcctggtcgt agatggtgaa cgcggagggg
     3661 tagccgagca gggtggcgtc gcggcgcagg atccgcacgc acgccgagtg gaacgtggag
     3721 acccacatct tctccgcgac cggtccgacc agccggccga tgcgctcctt catctctccc
     3781 gccgccttgt tggtgaaggt gatggcgagg atctcgaacg gcgagacgcc gtggaccttg
     3841 atgaggtgcg cgatgcggtg cgtgaggacg cgggtcttgc ccgacccggc gcccgcgacg
     3901 acgagcaggg gcccgtcgcc gtgggtgacc gcctcgtact gggccgggtt caggccctcg
     3961 aggaggtcgt ccgcgtccat cgcggccacc ctacctgcgg cccccgacac gcccttcccg
     4021 ggtgtcgacc gctccggcgg cgcggcggcg tcaggtgaca gagcggcggc gtcaggtgcc
     4081 ggcgaggcgg ctgatgagcg gcgccaggtc gtcgagcgac gacgccgaca ggccgatggc
     4141 cgagatgccg aaccgctcgc ggcgctcctc gaggtcatcg gcgatctgat cgagggtccc
     4201 gcacagcgcg tgcggcgatc cggccgcctg ctcggcggtc atgccgaacc cgccggcgag
     4261 ggcgtcgaac atctcctggc ggtcgttcgt gacgatggcg aggtggatgc gggcgccgag
     4321 ctcgatgccg tcgaagcggt caccggccgc ggcgcggatc cagccgatct tctgctcggt
     4381 ggcctcgggg gtggccgacg gcccggcccg gtcgtcgatc accccggcgg gcagcgacgg
     4441 gttgaggttg atgacgtccg cgtggcggcc ggccacctcg agcaccttgc ggccgccgcc
     4501 gccgatgacg atcggcgggt gcggctgctg ggccgggctc ggcgtcccga ccagaccgga
     4561 gatgtcgtag aagcagccgt ggtgctcgac cggctcgccg gaccacagcg cccggatgac
     4621 ctcgagggcc tcctcgaggc gggcgatgcg caccgacggc gggtcgagcg ggatgccggt
     4681 ggcggtgtag tcggtggtca tccagccggc gccgaggccg aactccaggc ggccgccgct
     4741 gagccggtcg acggtggcgg cctccttggc cagcagcgcc gggtgccggt agtcgttggc
     4801 gaagacgagc gtgccgaccc gcagcgtggt ggtggcgtcg gccgcggcca tgagcgccgc
     4861 gatcggggcg agctggtcgt cgaggtgatc ggcgacggtc aggacgctga cgccgacgtc
     4921 ctcggcgcgg cgcgcgagcg ggacgaggtc ctcgggccgg gccatgtgcg aggcctggac
     4981 ggtgaagcga aacggacgag ccaccctcgg aatatagggg tcgcgatcga caacccttgt
     5041 cagaatcccg accacagaat ccgcacggcc gacctccggt acggtgcgcg ccgtgaccga
     5101 ttccacagcc ggcgacgctc cggccgacaa cacgcccgcc atcgacgggt tccgcgccga
     5161 gacgttcacg ttcgcgtccc gcgcccgcac gatctacacc ggcggcgacg gccccgcggt
     5221 gatcgtcatg gccgagatgc ccggcatcac cccggcggtc atcgcgttcg cccgccgggt
     5281 gatcgacgcc gggttcaccg tggtcatgcc ggtgctgttc ggcgaaccgg gccgcgacat
     5341 ctcgctgccg tacatggcga cgtcgctcgc gccggcgtgc gtgtcgaagg agttccgggc
     5401 cttcgcgctg cggaccactc ccccggccat cgactggttg cgggcgctgg cccgccattg
     5461 ccacgaacgc cacggcggcc ccggggtggg ggcggtgggc atgtgcttca ccggcgggtt
     5521 cgccctggcg atggccaccg agcccgagat gctggccccc gtgctcagcc agccgtcgct
     5581 gcccatcggc ggcgccaagc ggaaggccga cctgggcctg tcggatgctg acctgatggc
     5641 ggtgcgcgac cgggtcgact ccgacgacat ctgcgtgctc ggcctgcgct tcaccggcga
     5701 caagctggcc ccgccggagc ggttcgcgtc gctgcgccgc gagctcggcg agaacttcat
     5761 cggcgtcgag atcgactcgt cgccgggcaa cccgtggggc caccgccgcc tggcgcactc
     5821 ggtgctcgcc gaggacctgg tcgacgagcc cggacagccg acccacgagg cgctcgagca
     5881 ggtgct
//
LOCUS       DAOQOD010000185         5845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5415_length_5845_cov_11.240587, whole genome shotgun sequence.
ACCESSION   DAOQOD010000185 DAOQOD010000000
VERSION     DAOQOD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_5845_cov_11.240587"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PLZ72_13785"
     CDS             complement(<1..975)
                     /locus_tag="PLZ72_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNA37416.1"
                     /translation="MPRRDDLESILIIGSGPIVIGQACEFDYSGTQACRVLREEGYRV
                     ILANSNPATIMTDPDFADATYVEPLVAEVLEKILDVERPDAVLPTLGGQTALNLAMEL
                     SESGALERRGIELIGADADAIATAEDRDRFKSAMLEIGLDVPASGIAHSMDEARAVVA
                     ELGLPVVIRPAYILGGKGTGIASTPEEFEQRASFGLDASPITEILIERSIAGWKEYEL
                     EVMRDRADNCVVICSIENVDPMGVHTGDSITVAPAQTLTDVEYQAMRDAAFACIRRVG
                     VETGGSNVQFAVDPATGDQVVIEMNPRVSRSSALASKATGFPIAKIAAK"
     gene            1044..1541
                     /locus_tag="PLZ72_13790"
     CDS             1044..1541
                     /locus_tag="PLZ72_13790"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNA37417.1"
                     /translation="MKFGFEQRWTAPVDAIMETYLDEGFWNGLDGFSNMEPPQLLSLE
                     RDDAAGTALVRLRYKVNATLPAQAARFIDPDDVAWVQETEWDLTAVTAQVRFLPVQAA
                     GLMRASASIDVRTDGADTVRELRGEFKVKIPLVGGRVEKAVVGDIGTNLEEEADAVAE
                     HLGID"
     gene            complement(1576..2682)
                     /gene="carA"
                     /locus_tag="PLZ72_13795"
     CDS             complement(1576..2682)
                     /gene="carA"
                     /locus_tag="PLZ72_13795"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNA37418.1"
                     /translation="MSRGIEEAAIVLADGTLFEGEAIGARPEGGVTPGEFVFNTVLSG
                     YQEVISDPSYAGQVITFTYPHIGNYGVNAHDDESVRPHCSGVVVRELARRHSNHRAEG
                     DLDSYLRGFGVAGIAGVDTRRLTRHLRDHGAMPGVFGSADEATLRSAASAAVGTDGRD
                     LVAEVTAAGVASYPATGGAAAASRRVVALDLGIKTTIVRSLTTVASVEVVPATTSAAD
                     ILALSPDGVFLSNGPGDPAAVAVVPDTVRELLGRVPVFGICMGHQMIGTALGGRTAKL
                     PFGHHGGNHPVRDLGTGRVEITSQNHNYVVDPDSLDRVEVTHVNLNDGTLEGFRCLDV
                     PAFAVQYHPEAGPGPHDSHYLFGRFADMIDEHRR"
     gene            complement(2744..4117)
                     /locus_tag="PLZ72_13800"
     CDS             complement(2744..4117)
                     /locus_tag="PLZ72_13800"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNA37419.1"
                     /translation="MSEIRPGADVVIKGGRVLDAGGERPADVVVSNGRIAAVGADLDA
                     EVVLDAGGCLVTPGLVDLHTHLREPGREEAETVESGARAAALGGYTAVLAMPNTTPTM
                     DSAAVVREVLELGRSSVCDVHASGAITVDRAGEQLAPMAEMAALGVRIFTDDGTGVQD
                     DRLMRRALEYAAGLGVTLAQHCEVSALSAGTCMHEGEWSARLGLPGQPAEAEELMVAR
                     DIALARLTGARMHFLHLSTAGSVALVAAAKASGLAVTAEAAPHHFTLTDACCAGYDAT
                     FKVHPPLRPDVDVDAVASGLADGTIDAIATDHAPHPLEEKERPFDQAPPGMLGLEYAL
                     ALALTRLVDGQRADVDPDRPDGGGVAGAAGPYRGRRLSDADVIARLSWQPAAIAGMED
                     HGGPIEQGRAANLCVVDTAHRWTIDGSGGASRSRNVPYVGLNVRGRVRHTVCRGEPVV
                     VDGEVQR"
     gene            complement(4114..5118)
                     /locus_tag="PLZ72_13805"
     CDS             complement(4114..5118)
                     /locus_tag="PLZ72_13805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNA37420.1"
                     /translation="MDGHLLSISDLTRADLEEVLDVTDSMVEVSSRAIPKVPALRGRT
                     VVSLFYEDSTRTRLSFDTAAKRLSADTMSFAVSSSSVNKGESVRDTIETIEAMGIDAV
                     VVRHRSSGVPAQIAAWTDATVINAGDGWHQHPTQALLDSYTIRSHRGSLDGLHIGIVG
                     DIKHSRVARSDIEAFTRLGAHVTLVAPPTLLPASTAPWDVQVSHDLDSVLGSLDVCYL
                     LRMQRERMTEALVPTLREYTACYGLSAERADRLPDGALIMHPGPMNRGVEIASEVADR
                     PDAVITEQVANGVAARMAVLFLLLGPGRAALPPLADGGVNGGVGDAGTPTPSTSGAST
                     "
     gene            complement(5127..5714)
                     /gene="pyrR"
                     /locus_tag="PLZ72_13810"
     CDS             complement(5127..5714)
                     /gene="pyrR"
                     /locus_tag="PLZ72_13810"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNA37421.1"
                     /translation="MAERERRLTRPYGGVFVARSRVMNAEDIQRALRRMSHEILERNH
                     GLDELVVIGLQTGGVDLAKALAAALGDIEGLGPDDPEVPVGTLDVALHRDDIGIRPVL
                     PEEITEIPVDLDGRTVVLVDDVLFTGRTIRAALEAMGTYGRPRAVQLAVMIDRGHREL
                     PIRPDYVGKNLPTRRDELVDVTMAGVELGEIHKPG"
BASE COUNT          795 a         2027 c         2168 g          855 t
ORIGIN      
        1 cttggcggcg atcttggcga tcgggaagcc ggtggccttc gacgccagcg ccgaggaccg
       61 gctcacccgg gggttcatct cgatcacgac ctggtcgccg gtggcggggt cgaccgcgaa
      121 ctggacgttg gatccgccgg tctccacccc gacgcggcgg atgcaggcga acgcggcgtc
      181 gcgcatggcc tggtactcga cgtcggtcag ggtctgggcc ggggccacgg tgatcgagtc
      241 gccggtgtgc acgcccatcg gatcgacgtt ctcgatcgag cagatgacga cgcagttgtc
      301 ggcccggtcg cgcatgacct cgagctcgta ctccttccag ccggcgatcg agcgctcgat
      361 gaggatctcg gtgatcgggc tggcgtcgag gccgaacgag gcccgctgct cgaactcctc
      421 gggggtcgag gcgatcccgg ttcccttgcc gcccaggatg taggcggggc ggatcacgac
      481 cggcaacccc agctcggcga ccacggcgcg ggcctcgtcc atgctgtggg cgatgccgga
      541 cgcggggacg tccaggccga tctccaacat ggcggacttg aagcggtccc ggtcctcggc
      601 ggtggcgatc gcgtcggcgt ccgcgccgat cagctcgatg ccgcgacgtt cgagcgctcc
      661 ggactccgac agctccatgg ccaggttgag cgcggtctga ccgccgaggg tgggcagcac
      721 ggcgtcgggc cgttcgacgt cgaggatctt ctcgagcacc tcggcgacca gcggttcgac
      781 gtaggtggcg tccgcgaagt cggggtcggt catgatcgtg gccgggttcg agttggccag
      841 gatgacccgg tagccctcct cgcgcagcac ccggcacgcc tgggtgccgg agtagtcgaa
      901 ctcgcaggcc tggccgatca cgatcggacc ggacccgatg atcaggatcg actccaggtc
      961 gtcacgacgg ggcatgtggg gtcctcttgt tccgggtggg ggtgttcggg atgtgctggt
     1021 tcgggatgtg cgacattggt gcggtgaagt tcggattcga gcaacggtgg accgctccgg
     1081 tcgacgccat catggagacg tacctcgacg aggggttctg gaacggtctg gacgggttct
     1141 ccaacatgga gccgccgcag ctgctgagcc tcgagcgcga cgacgcggcg ggaacggcgc
     1201 tggtgcgcct gcggtacaag gtgaacgcca cgctgcccgc ccaggccgcc cgcttcatcg
     1261 accccgacga cgtcgcgtgg gtgcaggaga ccgagtggga cctgacggcg gtgaccgcgc
     1321 aggtgcggtt ccttccggtg caggccgccg ggctgatgcg ggcatcggcg agcatcgacg
     1381 tgcgcactga cggcgccgac accgtccggg agctgcgcgg cgagttcaag gtcaagatcc
     1441 ccctcgtcgg cggtcgggtc gagaaggccg tggtgggcga catcggcacc aacctcgagg
     1501 aggaggccga cgccgtcgcc gagcacctcg gcatcgactg aacgtcgcgc gccgggcgtc
     1561 gtcgggagcg cccgatcacc gacggtgctc gtcgatcatg tcggcgaacc gcccgaacag
     1621 gtagtggctg tcgtgcggac ccgggccggc ctcggggtgg tactgcacgg cgaaggcggg
     1681 aacgtccagg cagcggaagc cctcgagggt cccgtcgttg aggttgacgt gggtgacctc
     1741 gacgcggtcg agcgagtcgg ggtcgacgac gtagttgtgg ttctggctgg tgatctccac
     1801 ccggccggtg ccgaggtcgc gcaccgggtg gttgccgccg tggtgaccga agggcagctt
     1861 ggcggtccgc ccgccgagcg cggtgccgat catctggtgc cccatgcaga tcccgaacac
     1921 cggcacccga cccaacagct cgcgcacggt gtcgggcacg accgccaccg cggccgggtc
     1981 gcccggcccg ttggacagga acaccccgtc gggtgacagc gccaggatgt ccgccgccga
     2041 ggtcgtggcc ggaaccacct cgaccgaggc gacggtggtg aggctccgca cgatcgtggt
     2101 cttgatgccc aggtcgaggg ccaccacccg acgagaggcc gccgccgccc cgccggtcgc
     2161 cgggtaggag gcgacgcctg ccgcggtcac ctcggcgacc aggtcccgtc cgtcggtgcc
     2221 gaccgcggcg ctcgccgcgg aacgcagcgt ggcctcgtcg gctgaaccga acacgccggg
     2281 catggccccg tggtcgcgca ggtgtcgggt gaggcgacgg gtgtcgaccc cggcgatgcc
     2341 ggcgacgccg aagccccgca ggtacgagtc gaggtcgccc tcggcccggt ggttgctgtg
     2401 gcgccgggcc agctcacgca cgaccacccc ggagcagtgc gggcggaccg actcgtcgtc
     2461 gtgcgcgttg accccgtagt tgccgatgtg ggggtaggtg aacgtgatca cctgcccggc
     2521 gtaggacggg tcggagatga cctcctggta gcccgacagg acggtgttga acacgaactc
     2581 gcccggggtc acccctccct cgggacgggc gccgatcgcc tcgccctcga acagggtgcc
     2641 gtcggccagg acgatggccg cttcctcgat gcctcggctc aacgcctcgc tccttcgctc
     2701 gtgtcggtgg tgggcccggt cacgggcccg gcgccgctgc tgctcaacgc tggacctcgc
     2761 cgtcgacgac gaccggttcg ccccggcaca cggtgtggcg cacccggccc cgcacgttca
     2821 ggccgacgta cgggacgttg cggctgcgcg acgcgccgcc ggatccgtcg atcgtccagc
     2881 ggtgtgcggt gtcgacgacg cacaggttgg cggcccgccc ctgctcgatc ggaccgccgt
     2941 ggtcctccat ccccgcgatg gcggcgggct gccacgacag gcgggcgatc acgtccgcgt
     3001 cggagagccg ccgtccccgg tacggacccg cggcaccggc gactccgccg ccgtcgggac
     3061 ggtcgggatc gacgtccgcg cgctggccgt cgaccaggcg ggtgagcgcc agcgccaggg
     3121 cgtactcgag gccgagcatc cccggagggg cctggtcgaa cggacgctcc ttctcctcga
     3181 gcgggtgcgg ggcgtggtcg gtggcgatgg cgtcgatggt gccgtcggcc aggccggacg
     3241 ccaccgcgtc gacatccacg tccggacgca ggggcgggtg gaccttgaag gtggcgtcgt
     3301 agcccgcgca gcaggcgtcg gtgagcgtga aatggtgcgg cgccgcctcg gcggtcaccg
     3361 ccagccccga cgccttggcc gccgcgacca gcgccaccga acccgcggtc gacaggtgca
     3421 ggaagtgcat ccgagctccg gtgagtcggg ccagggcgat gtcgcgggcc accatcagct
     3481 cctcggcctc ggcgggctgg ccgggcaggc cgaggcgggc cgaccactca ccctcgtgca
     3541 tgcaggtgcc ggcgctgagg gcgctgacct cgcagtgctg ggccagggtc acaccgagac
     3601 cggcggcgta ctccagcgcc cgccgcatga gccggtcgtc ctgcaccccg gtgccgtcgt
     3661 cggtgaagat cctcacgccg agagccgcca tctcggccat gggcgcgagc tgctcccccg
     3721 cccggtcgac ggtgatcgcg cccgaggcgt ggacgtcgca cacgctggac cggccgagct
     3781 cgagcacctc gcgcacgacc gccgcgctgt ccatggtcgg cgtggtgttg ggcatggcca
     3841 gcaccgcggt gtagccgccg agcgcggcgg cccgggcgcc ggactcgacc gtctcggcct
     3901 cctcccggcc cggctctcgc aggtgcgtgt gcaggtcgac cagtccgggc gtgaccagac
     3961 atcctccggc gtcgagcacg acctcggcgt cgaggtcggc tccgaccgcg gcgatccgcc
     4021 cgttcgacac caccacgtcc gcgggtcgct cgccgccggc gtcgaggacg cggccgccct
     4081 tgatcaccac gtcggcaccg ggccggatct cgctcacgtg ctcgctcctg aggtcgaggg
     4141 ggtgggggtc ccggcgtcgc cgacgccgcc gttcacgccg ccgtcggcga gcggcggcag
     4201 cgcggcccga cccggtccga gcagcaggaa cagcaccgcc atgcgcgcgg cgacgccgtt
     4261 ggcgacctgc tcggtgatca ccgcgtcagg gcggtcggcc acctccgacg cgatctcgac
     4321 gccgcggttc atcgggccgg gatgcatgat cagggcaccg tcgggaaggc ggtcggcccg
     4381 ctcggcgctc agcccgtagc aggcggtgta ctcgcgcagc gtcggcacga gggcctcggt
     4441 catccgctcg cgttgcatgc gcagcaggta gcagacgtcg agcgagccga ggacggagtc
     4501 gaggtcgtgg gagacctgca cgtcccacgg cgccgtcgac gcgggcagca gcgtgggggg
     4561 cgcgaccagg gtcacgtgcg ctcccagccg ggtgaaggcc tcgatgtccg accgtgccac
     4621 ccgggagtgc ttgatgtcgc cgacgattcc gatgtgcagc ccgtcgagcg aaccccggtg
     4681 gctgcggatc gtgtagctgt cgagcagggc ctgggtgggg tgctggtgcc agccgtcgcc
     4741 ggcgttgatg accgtggcgt cggtccacgc ggcgatctgc gccggtacgc ccgacgagcg
     4801 gtggcggacg accacggcgt cgatgcccat cgcctcgatc gtctcgatgg tgtcgcggac
     4861 cgactcgccc ttgttcaccg acgacgacga caccgcgaag ctcatcgtgt cggccgacag
     4921 gcgcttggcc gcggtgtcga agctcaaccg ggtgcgggtc gagtcctcgt agaacagcga
     4981 cacgacggtt cgaccccgca gggccgggac cttggggatg gcccgggagc tgacctcgac
     5041 catcgagtcg gtgacgtcga ggacctcttc gaggtcggcc cgggtcagat ccgagatgga
     5101 gagcaggtga ccgtccatga ccctcctcac cccggcttgt ggatctcgcc gagctcgacg
     5161 ccggccatgg tgacgtcgac cagctcgtcg cgtcgggtgg gcaggttctt gccgacgtag
     5221 tcggggcgga tcggcagctc ccggtggccg cggtcgatca tcaccgccag ctgcaccgcc
     5281 cgcggccgcc cgtaggtccc catcgcctcg agagcggcgc ggatcgtccg gccggtgaac
     5341 aggacgtcgt cgacgaggac gacggtgcga ccgtcgaggt cgacgggtat ctcggtgatc
     5401 tcctcgggca gcaccgggcg gatgccgatg tcgtcgcggt gcagcgccac gtcgagggtg
     5461 ccgaccggga cctcggggtc atcgggcccc aggccctcga tgtcgccgag ggcggcggcc
     5521 agggccttgg ccaggtccac gcccccggtc tgcagtccga tcaccaccag ctcgtcgagg
     5581 ccgtggttgc gttcgaggat ctcgtgggac atccgacgca gcgcccgctg gatgtcctcg
     5641 gcgttcatca cccgtgaccg ggcaacgaaa acgcccccgt aggggcgcgt caggcgtcgc
     5701 tcgcgttctg ccaccggcag gtccttcctg tccgtagggg cctcacggga cccccttcac
     5761 ggtcaggccc gcaccttagc agctgcccgg caggccgtat caggccgtat caggccggat
     5821 caggcgagcc ggagctcagg cgggt
//
LOCUS       DAOQOD010000186         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5428_length_5839_cov_12.880878, whole genome shotgun sequence.
ACCESSION   DAOQOD010000186 DAOQOD010000000
VERSION     DAOQOD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5428_length_5839_cov_12.880878"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLZ72_13815"
     CDS             <1..630
                     /locus_tag="PLZ72_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNA37422.1"
                     /translation="PPFSVRIRNATMTAHRAAERKQFVHRLMRGQLPIDGVADLMVQH
                     HAIYRALEQTGDGLADDAVVGPFVGAELRRVPRIEADLANLVGADWSERFGTRPATRA
                     YVELIRSSGAEPARFVAQHYTRLLGDLSGGQAIRRTLERHYGERLAGSLSFYDFDIDD
                     LDAYKDAYRARLDDAPWADAELDAFIAETNRAYDANSAMFVELDEAWPA"
     gene            791..1169
                     /gene="rnpB"
                     /locus_tag="PLZ72_13820"
     ncRNA           791..1169
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLZ72_13820"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1189..1869)
                     /locus_tag="PLZ72_13825"
     CDS             complement(1189..1869)
                     /locus_tag="PLZ72_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37423.1"
                     /translation="MVVERLDAEHVCTVHVYTVHVYTVDTEWYRRRIATLTRSTVVAA
                     ARARVAEHGLPSLSMRSLADDLAVTPMAIYRHVANREGLLDAIVDDALDRLGDIDEGL
                     APEVLMRRLESRLVDAFGDLPELALLLAHRGPRTERSVAVIGRVVHALVDGGLDAEVA
                     ARTYATMSTLALVRIAAAAAGSGSTASLVGGGPDAKLATLAVDQLSRSDGSTVDAVLE
                     AAHALRSV"
     gene            1825..2190
                     /locus_tag="PLZ72_13830"
     CDS             1825..2190
                     /locus_tag="PLZ72_13830"
                     /inference="COORDINATES: protein motif:HMM:NF039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6506 family protein"
                     /protein_id="HNA37424.1"
                     /translation="MYGADVFGVETLDDHGGSTMQRWGFIYTLGADATTRRVDVIGSP
                     TCELIAVGVASPADGADAATWLVDQGAELVEMCGAFGPADQASVAAALSRSAAEHPVP
                     HGAVTYPCDQAAGLHRLFG"
     assembly_gap    2855..2954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2955..4213
                     /locus_tag="PLZ72_13835"
     CDS             <2955..4213
                     /locus_tag="PLZ72_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNA37425.1"
                     /translation="TAPTTTAPTTTAPGGVTGPAITTTITAGKGGEPIEGVVITVSRD
                     GEQIGRATSGPDGTLTIPVPGSGQYQVTLDKKSLPDGVQVQKGAPGTLTPFVQASGSL
                     PVVFRLTEAGQADSGGGPSDVERLTSLAASGIRFGLVIALAAVGLSLIFGTTGLTNFA
                     HGELIAFGAIVTWYLNAPNGGLGLPLLVAAVGGILASGLFGASFELGVYRPLRRRGMP
                     VVSQMVVSIGLAFALRYLFSIVFGQSPQQYAQYAAQSPTVSLGPVSLRPKDLVITAVA
                     FACLIAIGLFLQRARLGTAIRAVSDNRDLSIASGIDDQRVILIVWVVAGALAGLSGIM
                     LASSDSTSWNMGQRVLLVTFAAVVLGGLGTTFGAMVGGLVVGIVSDLSTFWLDADLKI
                     VVALATLVVVLLIRPQGIFGVRTRTA"
     gene            4227..5204
                     /locus_tag="PLZ72_13840"
     CDS             4227..5204
                     /locus_tag="PLZ72_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNA37426.1"
                     /translation="MNLGAALVDGLRDMVAVPSVVYALGAIGLNIQFGFAGLLNFGSV
                     ASGLVGAYGASIAVDQGLPLWVGVIIGLAAAALLGLLLGLPTLRLRADYLAIATISAA
                     EIIRVIVNSARLQDVTGGPIGLTGGADDFFAVNPIPEGRYGWGSFSFNQNTLWTILVG
                     WTIVIVLCLFVWGLARSPWGRLLKAVREDEDAARSLGKNVFKLKLQAFVLGSVIGGIA
                     GIIFMFDGGFVKPDFFVSQTTFNWYLVVILGGVATVLGPPVGAIVYWFVISVLNSVLT
                     QVIGDGWWIFDTTDTGAIRYVLVGLTIVLLLVFRPQGLLGDKDEAVIGE"
     gene            5197..>5839
                     /locus_tag="PLZ72_13845"
     CDS             5197..>5839
                     /locus_tag="PLZ72_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA37427.1"
                     /translation="MSDTTHAPDDGPILIADGVHRSFGGVRAVDVEHMEVERGSVVAL
                     IGPNGAGKTTFFNVLTGFERADGGTWRFEGHDVSGQAAYAAARVGMVRTFQTARVFPR
                     LSVLDNIRLASKDQKGEHLRSALFPWVWRRQEAELGERAAELGRWVGLGDKLEDRAGT
                     LSGGQRKLLELARGVMAAPRLLMLDEPMAGVNPALRQLLLDKIRELPGEGITVL"
BASE COUNT          822 a         2044 c         1992 g          881 t
ORIGIN      
        1 ccgccgttct cggtgcggat ccgcaacgcg acgatgaccg cgcaccgggc cgctgagcgc
       61 aagcagttcg tccaccgcct gatgcggggc cagctcccga tcgacggcgt ggccgacctg
      121 atggtccaac accacgcgat ctaccgggcc ctcgagcaga ccggggacgg cctggccgac
      181 gacgcggtcg tcggcccctt cgtcggagcg gagctgcgtc gggtcccgag gatcgaggcc
      241 gacctggcga acctggtggg agccgactgg tccgagcggt tcggcacccg cccagcgacg
      301 cgggcgtacg tcgagctgat ccgctcgagc ggcgcggagc cggcacggtt cgtcgcccag
      361 cactacacga ggctcctcgg cgacctctcg gggggccagg cgatccgtcg caccctcgag
      421 cgtcactacg gcgagcggtt ggccggttcc ctgtcgttct acgacttcga catcgacgac
      481 ctggacgcgt acaaggacgc gtaccgggcc cgattggacg acgccccgtg ggccgacgcc
      541 gagctcgacg ccttcatcgc cgagaccaac cgggcctacg acgccaactc ggcgatgttc
      601 gtcgagctcg acgaggcctg gcccgcctga tcccgctccc ccgccgaact tgtagcccac
      661 aggccgcccc acgacccacc agctacaaga tcgtcgcacc ccgccgaact tgtagcccac
      721 cggtcgtctc acggcatgcc cgctacaagt tcggcggggt ggccgggtgc tggcacactg
      781 ggagcgctgg tgagtcgtcc gggtggccgc ggcggcgcag acctcagggt ccgtgccgtc
      841 gaggaaggtc gggactccgc agggcagggt gctggcgaga gccaggtcgg agcgatctga
      901 cggatcgggc aacagagagc agaccgccga tggccgaccc ccggggtcgg cacaggcaag
      961 ggtgaaacgg tgcggtaaga gcgcaccatc gccgggggcg accccggcgg atggcaaccc
     1021 ccacccggag caaggccgag cagaggacgt ggacggcccg tccgcccttc ggggcactcc
     1081 tcgggtaggc cgcagagatg gatggtcacc catccggact cccgcgaggg gccggtggac
     1141 agaatcccgc ctacaggacg actcaccagc gccccgaccg cccgaggttc agacggaccg
     1201 aagggcgtga gccgcctcca gcaccgcgtc caccgtggat ccgtcggatc ggctgagctg
     1261 atcgacagcc agcgtggcca gctttgcatc cggtccaccg cccacgagcg aggccgtcga
     1321 gcccgagccg gcagcggcgg ccgcgatgcg caccagggcg agcgtgctca tggtggcgta
     1381 cgtgcgagcc gccacctccg cgtccagccc gccgtcgacg agggcatgca ccacccggcc
     1441 gatgacggcg accgagcgct ccgtgcgtgg accccggtgg gcgagcagca gggcgagctc
     1501 gggcaggtcg ccgaaggcat cgacgagccg tgactcgagc cgccgcatga gcacttcggg
     1561 agcgaggccc tcatcgatgt cgccgagtcg gtcgagcgca tcgtcgacga tcgcgtcgag
     1621 aagaccctct cggttggcga cgtggcggta gatggccatc ggggtgacgg ccaggtcatc
     1681 tgccagcgac cgcatgctga ggctcgggag cccgtgttca gcgactcgcg ctcgggcggc
     1741 ggccacgacg gtggatcggg tgagggtggc gatacgccga cggtaccact cggtgtctac
     1801 ggtgtagacg tgtacggtgt agacatgtac ggtgcagacg tgttcggcgt cgagacgctc
     1861 gacgaccatg gaggatcgac gatgcagcga tggggtttca tctacacgct gggagccgat
     1921 gcaacgaccc gacgggtcga cgtcatcggc tcaccgacct gcgagctgat cgctgtcgga
     1981 gtcgcctccc ccgccgacgg agccgatgcc gccacgtggc tggtcgacca gggcgccgaa
     2041 ctggtcgaga tgtgcggcgc cttcggccct gccgatcagg cctcggtcgc cgccgccctg
     2101 tcccgctcgg ccgccgagca cccggttccg cacggcgcgg tgacctaccc gtgcgaccag
     2161 gccgcggggc tccaccgcct cttcggctga tcctccactg caggcgccca ccggtccccg
     2221 accggtcccc ggacgcggac gagccccggt ccaaggcggg agaggaaggt ggaccagggc
     2281 tcgtcaccac ggccgttgcg aaccgtgggg gtgatgatga accggtcgta cccggcggcc
     2341 acccatttca tgcacacacg ccgtgtgaca tccgtcacgc ctggcggacg ggccgggggt
     2401 cgcacggtcg gggatctccg cccggacacg cagaggggtc cccggccctg ggaccgggga
     2461 cccccactgg tggtcgcccc accaccgaca ggtacaacgg cacaccgacg cttcgcgttg
     2521 acaggcagtg ttacccaatg cgctgttccc caatgcgcct gttacccgat gcgctgttgc
     2581 ccaacggcgc cgacctggct caccgaccgc tcaggatcca ccggcgctgc atctgtccga
     2641 ccggacgtcc cgcatcatcg cccccaccgc tgcgatgtcc gtatgattcg gcgccgatgg
     2701 acgaggtacg ggacggggca ccacctcgag gtgggcggcg ggtggtgctc gtcgccctgc
     2761 tcgccctcgt cgcgtcggcc tcgctgctct cctcgctggc cggcgcccag gagccgaacc
     2821 ccaccgctcc gaccaccacc gctccgacca ccacnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnccaccg ctccgaccac caccgctccg accaccacag ccccgggcgg
     3001 ggtcacgggt cccgccatca cgacgacgat cacggccggc aagggcggcg agcccatcga
     3061 gggcgtggtc atcaccgtca gccgtgacgg cgagcagatc ggccgggcga catccggtcc
     3121 cgacggcacg ctgacgatcc cggttcccgg gtcgggccag taccaggtca ccctcgacaa
     3181 gaagtcgctg cccgacggcg tccaggtcca gaagggcgcc ccgggcaccc tcaccccgtt
     3241 cgtgcaggcg agcggatcgc tcccggtcgt gttccgactg accgaggccg gccaggccga
     3301 ctccggcggc ggaccgtccg acgtcgaacg cctcaccagc ctggcggcca gcgggatccg
     3361 cttcgggctg gtcatcgccc tcgccgcggt ggggttgtcg ctgatcttcg gcaccacggg
     3421 actcaccaac ttcgcccacg gcgagctgat cgccttcgga gcgatcgtca cgtggtacct
     3481 caacgcaccc aacggaggcc tgggcctgcc gctgctggtg gccgcggtcg gcgggatcct
     3541 cgcgagcggc ctgttcggcg cgagcttcga gctcggcgtg taccgaccgc tgcgacgtcg
     3601 gggcatgccg gtcgtgtcgc agatggtggt gtcgatcggc ctcgccttcg ctctgcgcta
     3661 cctgttcagc atcgtgttcg gtcagtcccc ccagcagtac gcccagtacg ccgcgcagtc
     3721 gccgacggtg tcgctcgggc ccgtgtcgct gcggcccaag gacctggtga tcaccgccgt
     3781 cgcgttcgcc tgcctgatcg ccatcggcct gttcctgcag cgggcccggc tcggcaccgc
     3841 catccgggcg gtgtcggaca accgcgacct gtcgatcgcg tccggcatcg acgaccagcg
     3901 ggtcatcctg atcgtgtggg tggtcgccgg tgcgctggcg ggcctgtcgg ggatcatgct
     3961 ggccagctcc gactcgacgt cgtggaacat gggccagcgg gtgctgctcg tcaccttcgc
     4021 cgcggtggtt ctcggtggcc tgggaacgac cttcggcgcc atggtcggcg ggctggtcgt
     4081 ggggatcgtg tcggacctgt cgacgttctg gctcgacgcg gacctgaaga tcgtcgtggc
     4141 gttggcgacc ctggtcgtcg tgctgctcat ccgcccgcag gggatcttcg gcgtgaggac
     4201 gcgaaccgca tgagcgaggc ccgacgatga acctcggcgc cgcgctcgtc gacgggctcc
     4261 gcgacatggt cgcggtcccc agcgtcgtgt acgcgctcgg agcgatcggg ctcaacatcc
     4321 agttcgggtt cgccggcctg ctgaacttcg gctccgtcgc ctcgggcctg gtcggcgcgt
     4381 acggcgcctc gatcgcggtg gaccagggcc ttccgctctg ggtcggcgtc atcatcggac
     4441 tggctgccgc cgccctgctc ggactcctgc tcgggcttcc caccttgcgg ctgcgcgccg
     4501 actacctggc catcgccacc atctcggcgg ccgagatcat ccgggtgatc gtgaactccg
     4561 cacgtctcca ggacgtgacc ggcggaccga tcgggctgac cggcggcgcg gatgacttct
     4621 tcgccgtcaa cccgatcccc gaagggcgct acggctgggg gtcgttctcg ttcaaccaga
     4681 acacgctgtg gacgatcctc gtcgggtgga cgatcgtgat cgtgctgtgc ctgttcgtgt
     4741 ggggtctcgc ccgcagcccg tggggccgtc tgctcaaggc ggtccgcgag gacgaggacg
     4801 cggcgcggtc gctcggcaag aacgtgttca agctgaagct gcaggcgttc gtcctcggca
     4861 gcgtgatcgg cggcatcgcc ggcatcatct tcatgttcga cggcgggttc gtgaagccgg
     4921 acttcttcgt ctcgcagacc acgttcaact ggtacctggt cgtcatcctg ggcggcgtcg
     4981 ccaccgttct gggccctccg gtcggggcga tcgtctactg gttcgtcatc tcggtcctca
     5041 acagcgtgct cacccaggtg atcggcgacg ggtggtggat cttcgacacg accgacaccg
     5101 gagcgatccg ctacgtgctg gtcggcctca cgatcgtcct gctgctggtg ttccgccccc
     5161 agggcctgct gggcgacaag gacgaggcgg tcatcggtga gtgacaccac ccacgctccc
     5221 gacgacggcc cgatcctgat cgcggacggg gtgcaccgct ccttcggtgg tgtgcgcgcc
     5281 gtcgacgtcg agcacatgga ggtcgagcgc ggcagcgtcg tcgccctcat cggtcccaac
     5341 ggcgccggca agaccacctt cttcaacgtc ctcacggggt tcgagcgggc cgacggcggc
     5401 acgtggcgct tcgagggcca cgacgtcagc ggccaggccg cctacgcggc ggcacgggtg
     5461 gggatggtcc gcacgttcca gaccgcccgg gtgttcccgc ggctctcggt gctcgacaac
     5521 atccgcctgg cctccaagga ccagaagggc gagcacctgc gctcggcgtt gtttccctgg
     5581 gtgtggcgac gccaggaggc cgagctgggc gagcgggccg ccgagctcgg ccgctgggtc
     5641 ggcctgggcg acaagctcga ggaccgggcc ggaactctgt cggggggtca gcgaaagctc
     5701 ctcgagctgg cccgcggcgt gatggccgcc ccccgactgc tcatgctcga cgagccgatg
     5761 gccggtgtga acccggcgct gcggcagctg ctgctcgaca agatccgcga gctgcccggc
     5821 gagggcatca ccgtcctgt
//
LOCUS       DAOQOD010000187         5774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5515_length_5774_cov_11.076237, whole genome shotgun sequence.
ACCESSION   DAOQOD010000187 DAOQOD010000000
VERSION     DAOQOD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5515_length_5774_cov_11.076237"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLZ72_13850"
     CDS             <1..362
                     /locus_tag="PLZ72_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNA37428.1"
                     /translation="VELIGTLDAAARDRILVSSFDMATLDRIKDVRPTLATGYLVFDL
                     NQQLDAAAVAADHGHDAFHPWDPFVTPELLEDCRSRGLRVNSWTVNEPERWSELVSWG
                     VDGIITDTPGRLVSALA"
     gene            complement(348..1337)
                     /locus_tag="PLZ72_13855"
     CDS             complement(348..1337)
                     /locus_tag="PLZ72_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNA37429.1"
                     /translation="MHILVVVNTAASAVTPRRRDAVLRHLALGHDLDVVGTERRGHAT
                     ELARSGLAAGIDAVAVLGGDGTVNEVATALVGTDTVLAPLPGGSTNVFVRSIGLPRDP
                     ALAAERTARALGRRRVDRIRVGTVDADGAGPRPFLCHTGIGWDAALVAEVERLRGRHR
                     RLRFTAHGGPRPATVPLYVRAGVRTFVSGWDRRRPSLHVGSSDPAVAVDGYFTLVMNS
                     DPYTFVGPWPLRVAPTADRHHPLTAVAVTDMSVGSFGPVVARALLGRRGLRPGRAVAL
                     HADLEELTVTAIGPHPLPFQADGDHLGEASQLVLASRPDALSVVDTRPPSGQR"
     gene            1444..2226
                     /locus_tag="PLZ72_13860"
     CDS             1444..2226
                     /locus_tag="PLZ72_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNA37430.1"
                     /translation="MNVVVCVKQIPDPAEPGALDPETKTLKRDGKLILDESDSYGVEM
                     ALQLVDAAGGGEVTLVSMAPNSEVGGLRTALAMGAAKAVLVSDDALKGTDALGTAKVL
                     AKAIGRAGDADLILTATESSDGYTGVVPEQIAELLGLPSVTFAKSITIDGGTAKVQRQ
                     TEAGYDDVEVPLPAVVSVTAGVVEPRYPSFKGIMAAKSKPVDEVTVADLGLDAGQVGW
                     AGAGQQITDVAQAEARQAGTIVEDDGEGFQKIVDFLAELKVI"
     gene            2271..3233
                     /locus_tag="PLZ72_13865"
     CDS             2271..3233
                     /locus_tag="PLZ72_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNA37431.1"
                     /translation="MALSKIWVFGEAAGGNVSGTTLELLTKARELAGTVEVVTTSGAE
                     LAGPAGAHGATKVLNVGGTDGKLVGVPVAAAIAAAVDAGNGPDAILFATSYDGRDVAG
                     RLSAKLDAPVITNVTDLVPAGDGLAGVEPIFGGSTDVTTAFTGDKVKIFLVRPKSFAA
                     EESGGAAAAVEELSVPDTGATGTAVITNRFVEEKTGPSLDDADIVVSGGRGLGEQTGF
                     EMIETLAKQLGAAPGASRAIVDAGWVPYSYQVGQTGKVVKPNVYIAAGISGATQHLVG
                     MKGSKYIIAINKDAEAPIFSVADLGIVGDVHKVLPKLIEALQSR"
     gene            3230..4606
                     /locus_tag="PLZ72_13870"
     CDS             3230..4606
                     /locus_tag="PLZ72_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNA37432.1"
                     /translation="MSTGSAPHGTHSEGPREGALGASGAGVRTWTNWAGNQSCRARLL
                     RPTSEPELCRIVAEAAARGHTARAVGSGHSFSDVALSASVLVDLSAYDRVLAIDRDAA
                     TVTVQSGARLADLSRSSWPRGMAFTNLGDIDVQTVAGATQTATHGTGVAFGNVSSAVV
                     GMRLIDGTGTVRVLDADHEPALFAAARAGLGALGLVSTVTVAMSPAFNLHAVHEPRRV
                     DEVLERFGELVRDNDHFEFFWVPGTGWAITKRHRRNHDPVAPRSRRDEWLQDELYDNV
                     LFGLANRVGRWRAGWGREVARRIPSPGRVEFNDRSYRVFASPRRVRFVEMEYAVPIEA
                     TVEAVRRVRALVDGLDYPVSFPVEVRTSAADDITLSTASGRATGWIAVHMYRGVPYEE
                     YFRAVEAIMVDLDGRPHWGKLHRRTPADLAPVYPRWDEFQELRARLDPSGVFQNAALD
                     RVLGPLGG"
     gene            complement(4678..5463)
                     /locus_tag="PLZ72_13875"
     CDS             complement(4678..5463)
                     /locus_tag="PLZ72_13875"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNA37433.1"
                     /translation="MSDDDRPFVLRRDDGAVVTLTLNRPESRNPLSLSMLEALDAHLA
                     DVAGDRDVRVVVIAGSGPAFSAGHDLRELHGTDDAEVHRRIFTRCSTVMQRIDSLPQG
                     VIARVNGVATAAGCQLVATCDLAVAGASARFATPGVNIGLFCSTPAVAVSRALAPKHT
                     MELLLTGELIGADDAFRMGLVNRVVPDEDLDAAAVELADRVAAAPLSVIAAGKAAYRR
                     QLGRSLADAYGIATEAMIDGLDRPEAIEGIGAFLEKRPPVWPT"
     gene            complement(5468..>5774)
                     /locus_tag="PLZ72_13880"
     CDS             complement(5468..>5774)
                     /locus_tag="PLZ72_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37434.1"
                     /translation="SGGARGRRRGAATGRFEAWWVLATLTAQDDDWPVDPGPVAADLR
                     WWMWEPGAHAGDVEPGWVCRLAVEDPLDGLAWALDATDRLVETSADGPGPDPLPSLP"
BASE COUNT          814 a         2120 c         2088 g          752 t
ORIGIN      
        1 ccgtcgagct gatcgggacg ctcgatgcgg cggcccgcga ccggatcctg gtgtccagct
       61 tcgacatggc gacgctcgac cgcatcaagg atgtccggcc cacgctggcc acggggtacc
      121 tggtgttcga cctcaaccag cagctcgacg ccgccgcggt cgctgccgac cacggccacg
      181 acgcgttcca cccctgggat cccttcgtca cccccgagct gctcgaggac tgccggtccc
      241 ggggcctgcg cgtcaactcc tggacggtca atgagccgga gcgctggtcg gagctggtct
      301 cctggggggt cgacggcatc atcaccgaca ccccgggccg cctggtctca gcgttggcct
      361 gacggggggc gagtgtcgac gacgctcagc gcatcgggcc gggaggccag caccagctgc
      421 gacgcctcgc cgaggtggtc gccgtcggcc tggaacggga gcgggtgcgg accgatcgcc
      481 gtgaccgtga gctcctcgag gtcggcgtgg agcgccacgg cccgtccggg ccgcagccct
      541 cgacgaccga gcagcgcccg ggcgacgacg ggaccgaacg acccgaccga catgtcggtc
      601 acggcgaccg ccgtcagcgg gtggtggcgg tccgcggtgg gagcgacccg cagcggccac
      661 ggtccgacga aggtgtacgg gtcgctgttc atcaccaggg tgaagtagcc atcgaccgcg
      721 accgccgggt cggacgatcc gacgtgcagg gacggacgcc ggcgatccca ccccgagacg
      781 aaggtccgca cgccggcccg cacatacagg ggaaccgtcg cggggcgtgg accaccgtgg
      841 gcggtgaacc gcagccgccg gtgccggcct cggagccgct ccacctcggc caccagcgcg
      901 gcgtcccacc cgatcccggt gtggcacagg aacggacggg gcccggcacc gtccgcgtcg
      961 acggtgccga cgcggatgcg gtcgacgcgg cggcgaccga gggcacgggc ggtgcgctcc
     1021 gcggcgagcg ccgggtcccg cggcagtccg atcgaccgca cgaacacgtt ggtcgatccg
     1081 cccggcagcg gggccaggac ggtgtcggtg ccgaccaggg cggtcgccac ctcgttgacg
     1141 gtgccgtcgc cgcccaggac cgcgaccgcg tcgatgccgg cggccagccc ggaccgggcc
     1201 agctcggtgg cgtggccgcg ccgttcggtt ccgacgacgt cgaggtcgtg gccgagcgcg
     1261 aggtggcgca gcacggcgtc gcgccgccgc ggcgtcaccg ccgaggccgc cgtgttcacc
     1321 acgaccagga tgtgcacccg gggagtctcc accgtcgtcg ccgtggcggg cggatcggcc
     1381 gccctggcgg cgacccgctc ccggttttgt gacggcgatc gggggcaagg cagacttcgg
     1441 cccatgaacg tcgtcgtgtg cgtgaagcag atccccgacc cggccgagcc gggcgcgctg
     1501 gatcccgaga ccaagaccct caagcgggac gggaagctga tcctggacga gtccgacagc
     1561 tacggcgtcg agatggcgct ccagctggtg gacgccgccg gcggcggaga ggtcaccctg
     1621 gtgtcgatgg cccccaacag cgaggtcggc ggtctgcgca ccgctctcgc catgggtgcc
     1681 gccaaggccg tgctcgtctc cgacgacgct ctcaagggca ccgacgcgct gggcaccgcc
     1741 aaggtgctgg ccaaggcgat cggccgggcc ggggacgccg acctgatcct gaccgcgacg
     1801 gagtcctccg acggctacac cggcgtcgtg cccgagcaga tcgccgagct cctcggcctg
     1861 ccgtcggtca ccttcgccaa gtcgatcacc atcgacggcg gcaccgccaa ggtgcagcgc
     1921 cagaccgagg ccggctacga cgacgtcgag gtgccgctgc ccgccgtggt gtcggtgacc
     1981 gccggtgtgg tcgagccccg ctacccgtcg ttcaagggca tcatggccgc caagtccaag
     2041 ccggtcgacg aggtcaccgt cgccgacctc ggtctcgacg ccggccaggt cggctgggcc
     2101 ggcgccggcc agcagatcac cgacgtcgcc caggccgagg cccgccaggc cggcaccatc
     2161 gtcgaggacg acggcgaggg cttccagaag atcgtcgact tcctggccga gctcaaggtc
     2221 atctgagcca ccggaccgac tgacccaacg agaagagaag agacgagaac atggctctgt
     2281 ccaagatctg ggtgttcggt gaggcggccg gcggcaacgt gtccggcacc accctcgagc
     2341 tcctgaccaa ggcccgcgag ctcgcgggca ccgtcgaggt cgtcaccacc tcgggcgctg
     2401 agctggccgg ccctgccggt gctcacggcg ccaccaaggt cctgaacgtc ggtggcaccg
     2461 acggcaagct ggtcggcgtg ccggtggccg ccgccatcgc cgccgcggtc gacgccggca
     2521 acggccccga cgccatcctg ttcgccacca gctacgacgg tcgcgacgtg gccggccgcc
     2581 tgtcggccaa gctcgacgct cccgtcatca ccaacgtcac cgacctggtg cccgccggcg
     2641 acggcctggc cggcgtcgag ccgatcttcg gtggcagcac cgacgtcacc accgccttca
     2701 ccggcgacaa ggtcaagatc ttcctcgtgc ggcccaagtc gttcgcggcc gaggagtccg
     2761 gcggcgccgc cgcggcggtc gaggagctgt cggtgcccga caccggcgcc accggcaccg
     2821 cggtcatcac caaccgcttc gtcgaggaga agacgggccc gagcctggac gacgccgaca
     2881 tcgtcgtgtc cggcggccgc ggcctgggcg agcagaccgg cttcgagatg atcgagacgc
     2941 tggccaagca gctcggcgcg gctcccggcg cctcccgcgc catcgtcgac gccggctggg
     3001 tgccctactc ctaccaggtc ggtcagaccg gcaaggtcgt gaagccaaac gtgtacatcg
     3061 cggcgggcat ctcgggcgcc acccagcacc tggtgggcat gaagggctcc aagtacatca
     3121 tcgccatcaa caaggatgcc gaggcaccga tcttctcggt ggccgacctc ggcatcgtcg
     3181 gcgacgtgca caaggtcctg cccaagctga tcgaggcgct gcagagccgg tgagcaccgg
     3241 ttccgctccg cacggaacgc actccgaggg cccccgcgag ggggccctcg gcgcgtccgg
     3301 ggccggggtg cgcacctgga ccaactgggc cggcaaccag tcctgtcggg cccggctgct
     3361 gcgcccgacc agcgagccgg agctgtgccg catcgtcgcc gaggccgccg cccggggcca
     3421 caccgcccgg gccgtcggct ccgggcacag cttcagcgac gtcgccctga gcgcctccgt
     3481 gctcgtcgac ctgtcggcgt acgaccgggt cctcgcgatc gaccgcgacg ccgccaccgt
     3541 gacggtgcag tcgggggcgc ggctcgcgga cctctcccgg tcgtcgtggc cgcggggcat
     3601 ggcgttcacc aacctgggcg acatcgacgt gcagaccgtc gccggggcca cccagaccgc
     3661 cacccacggc accggcgtcg cgttcggcaa cgtgtcgtcg gccgtcgtcg ggatgcggct
     3721 gatcgacggg acggggaccg tccgcgtcct cgacgccgac cacgaacccg cgctgttcgc
     3781 cgcggcgcgg gccgggctgg gtgccctcgg gctcgtgtcg acggtgaccg tggcgatgtc
     3841 gcccgcgttc aacctccacg ccgtgcacga gccgcgacgg gtcgacgagg tgctcgagcg
     3901 cttcggcgag ctggtccgcg acaacgacca cttcgagttc ttctgggtgc cggggacggg
     3961 ttgggcgatc accaagcgcc accgccgcaa ccacgacccg gtcgcgcccc ggtcccgtcg
     4021 cgacgagtgg ctgcaggacg agctgtacga caacgtgctg ttcggcctcg ccaaccgtgt
     4081 cggacggtgg cgggccggct ggggacggga ggtcgcccga cggatcccgt cgccgggtcg
     4141 ggtcgagttc aacgaccggt cgtaccgggt gttcgcgtcg ccgcggcggg tccgcttcgt
     4201 cgagatggag tacgcggtgc cgatcgaggc gaccgtcgag gcggttcggc gggtccgggc
     4261 cctggtcgac ggactcgact acccggtgtc gttcccggtc gaggtgcgca ccagcgcggc
     4321 tgacgacatc accctgtcga ccgcctcggg tcgggccacc ggctggatcg cggtgcacat
     4381 gtaccgcggc gtcccctacg aggagtactt ccgcgccgtc gaggcgatca tggtcgacct
     4441 cgacggccga ccccactggg gcaagctgca ccgacggacg cccgcggacc tggcgccggt
     4501 gtacccgcgc tgggacgagt tccaggagct gcgggcccgg ctcgacccgt ccggggtgtt
     4561 ccagaacgcc gcgctggacc gggtgctcgg ccccctcggc ggctgagtca actgctctgt
     4621 cctggctttg tcccgcacag gcgcggtgat cacggcgcct gtgcgggaca aagcgggtca
     4681 ggtcggccag accggcggcc gcttctcgag gaacgcgccg atgccctcga tcgcctcggg
     4741 acggtcgagc ccgtcgatca tcgcctcggt cgcgatcccg taggcgtcgg ccagggaccg
     4801 gccgagctgg cgtcggtagg ccgccttgcc cgcggcgatc accgacagag gtgccgcagc
     4861 gacccgatcg gccagctcga cggcggcggc gtcgaggtcc tcgtcgggga ccacccggtt
     4921 gaccagcccc atccggaacg cgtcgtccgc gccgatcagc tcgccggtca acagcagctc
     4981 catcgtgtgc ttgggcgcca gcgcccgtga caccgcgacc gcgggggtgg agcagaacag
     5041 cccgatgttg acccccgggg tggcgaagcg ggccgaggcc ccggccacgg ccaggtcgca
     5101 ggtggcgacg agctggcacc cggccgcggt ggccacgccg ttgacccggg cgatcacgcc
     5161 ctgggggagt gagtcgatgc gctgcatcac cgtcgagcac cgggtgaaga tccggcggtg
     5221 cacctcggcg tcgtcggtgc cgtgcagctc gcgcaggtcg tggccggcgc tgaacgcggg
     5281 gccggaaccg gcgatgacga cgacgcggac gtcccggtca ccggccacgt cggccaggtg
     5341 cgcgtcgagc gcttcgagca tcgacagcga cagcgggttt cgggactcgg gtcggttcaa
     5401 cgtcagcgtg acgaccgctc cgtcgtcgcg acgcagcacg aacgggcggt cgtcgtcgga
     5461 cacgggttca gggtagcgac gggagcgggt cgggtcccgg gccgtccgcg gacgtctcga
     5521 ccagccggtc ggtcgcgtcg agcgcccagg cgaggccgtc gagcgggtcc tcgaccgcca
     5581 gacggcacac ccaccccggt tcgacgtcac cggcgtgtgc gcctggctcc cacatccacc
     5641 accgcaggtc cgcggccacg gggcccgggt cgaccggcca gtcgtcgtcc tgcgcggtca
     5701 gcgtcgccag gacccaccac gcctcgaacc gtccggtcgc cgcgccccgt cgtcggccac
     5761 gggcaccccc cgat
//
LOCUS       DAOQOD010000188         5757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5547_length_5757_cov_12.262715, whole genome shotgun sequence.
ACCESSION   DAOQOD010000188 DAOQOD010000000
VERSION     DAOQOD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_5757_cov_12.262715"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..517)
                     /locus_tag="PLZ72_13885"
     CDS             complement(173..517)
                     /locus_tag="PLZ72_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37435.1"
                     /translation="MSSDTPGAAELEAATVALNRIAEWVSEGRAAFDRSDDRQLAMVF
                     LWANVGSQLKQYCRRLGIPAGTEPFAGPIQMRDKLVYGSVQGLDADVVRATCLVDGPE
                     LLELVTDLRAAL"
     gene            complement(514..909)
                     /locus_tag="PLZ72_13890"
     CDS             complement(514..909)
                     /locus_tag="PLZ72_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37436.1"
                     /translation="MQVLIVGTVSPDVTGAVGAPIRGQGRTQVKGTLLAGTVRAMDPA
                     PEAQMLRLAQALITLARGHGLSNLRLAGDGQIIADIADIAEGRTLLDVARFEIEAQAV
                     LQSRVSVLSSRAELAASVDNGPLAASSAA"
     gene            896..1090
                     /locus_tag="PLZ72_13895"
     CDS             896..1090
                     /locus_tag="PLZ72_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37437.1"
                     /translation="MSTCTSCGRDDEDTVAVRRVYLVLPDGAPTDPGSIEELAEATVV
                     DEDEDWCATCRDQYPHVLRS"
     gene            complement(1163..3253)
                     /locus_tag="PLZ72_13900"
     CDS             complement(1163..3253)
                     /locus_tag="PLZ72_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNA37438.1"
                     /translation="MLVVVAVGGLWAVLNTIQLPPAKPQDETTFVCDIEVQAGQCGFD
                     NAMARLSASEQRVVVTYNDLPPVLVQAVLSAEDRKFFDHNGVDPVGIARAFTKDVLGD
                     SASQQGGSTITQQYVKTVYLSSEKTLTRKIKEAVLAVKLEQTLDKREILTRYLNEIYF
                     GRGAYGVEAASRAYFGVGARDLDLPQAAYLAGLIRAPEHADATRDPKEATRRRKTVLD
                     AMVEEGYISESRARAAAEVPWNTEDKTSTGLPQQVTIRPRPAEHTDLGDVKYKELGSQ
                     FWMEWVRSQLRERLGPGAETKGLRVYTSFDPAMQKYAVDAVNKTLNQPDGPVGSLVAI
                     DTDGRVRAMYGGRDYETNKVNLALGSAGGGSGRAPGSTFKPFALAAFVEEGYSVKSRF
                     RAPPTTMFPNVYAEPGKLWKPGNYDKADHGVQSVEEATWDSTNTVYAGIVDTITPPRL
                     AEMANRLGVRAKLDPVYALVLGTEEVSVLDMASAYSTFADRGRHTEPYVITRIESSDG
                     DVIFDASTEVHPQQVISEQVADTVTTVLQGVIIKGTGAAAGLRTPAAGKTGTTNDAKD
                     AWFTGYTCNLTASVWMGYEQPKQMKSYKGQSVAGGTFPARIWRDFMSRAIAGDSACKF
                     PPADVGKKVLNSGMPLSSGASTTQPRASTTTTPGGASTSSSTPGASSTTVPRSTTTVA
                     PTTTAAPAAGGAGQ"
     gene            complement(3489..3899)
                     /locus_tag="PLZ72_13905"
     CDS             complement(3489..3899)
                     /locus_tag="PLZ72_13905"
                     /inference="COORDINATES: protein motif:HMM:NF036456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HNA37439.1"
                     /translation="MPFLAPESGGRPGTTPGSVDYRFARRRLLHRYRAGELTRADVCD
                     AQHELLRVGRNCAQRARHDCPVCGEHELRLARFVFGPRMPPGGRVVAGRAELLKLADR
                     HRGGGLRCYTVEVCLECRWNHLLDITPVGRRDSD"
     gene            4008..4817
                     /locus_tag="PLZ72_13910"
     CDS             4008..4817
                     /locus_tag="PLZ72_13910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNA37440.1"
                     /translation="MATSASATGRPRHPSAGNLGARPALVRDDSSPHRLPAASFPHGA
                     ENGRDRVRLGPDLDDDALDRLIGPVDGKRVLDLGCGAGAATIALARRGARVTAVEAST
                     TRLAQARAAAEVAEVRVEFHHSDLADLAFVRADTVDLVLAVYSLAGVQDLSRVFRQVH
                     RVLTPGSALLLSLPHPFTTMLDDDADQPSPYLTRTAWSASPLAWRAAGDEGVTHVHQI
                     GDVFTTLTRSNFAVDALLEPEPVAGVSGIHTSTLVEWVPPTLVVRARKLGI"
     gene            complement(4874..5578)
                     /locus_tag="PLZ72_13915"
     CDS             complement(4874..5578)
                     /locus_tag="PLZ72_13915"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNA37441.1"
                     /translation="MSRVDGSAQSLDEHALRCLPMLADHDRSTAIHAGRVTAYAVEIG
                     LELGLDPDELNDLRWAAALHDVGKVAVPPGILNKHGRLNDEELGVARAHPLIGMQMLD
                     PYRRWLGATVDGVAQHHERWDGHGYPFGLAGERISPLARIVTLADSFDAILSCRSYSE
                     PMTSLRARAEIVRCTGSQFDPGVVDALFARMDRALDDPDRLVPVEVVTAGDLMGIDWT
                     KLLLSPSPMTRAHAWT"
BASE COUNT          838 a         2074 c         1950 g          895 t
ORIGIN      
        1 cgccgccgac gcggtcgagt cgtgagcaga gtcgtgagcc atggcccacc tcctggtcag
       61 gtccagcgcc ccgtggctgc cggccgtcac caggaacggt aaatcaaccg atgggtccgg
      121 gaaaagcccc cggaccccgg tttcacccat cgacttgaac agcagcgacc gttcagaggg
      181 cggcgcggag gtctgtcacg agctcgagga gttcgggacc gtcgaccagg caggtggccc
      241 ggaccacgtc tgcgtccagc ccctgcaccg aaccgtagac cagcttgtct cgcatctgga
      301 tcggccccgc gaacggctcg gtaccagccg ggataccgag ccgtctgcag tactgcttga
      361 gctggctacc gacgttggcc cataggaaca ccatcgcgag ttgtcggtcg tcgctgcgat
      421 cgaacgccgc tcgtccctcc gacacccact cggcgatccg gttcagcgct accgtcgccg
      481 cctcgagctc agcagcgccg ggcgtatcgg agctcacgct gctgacgatg ccgccaacgg
      541 cccgttgtca acggacgccg ccaactcagc gcgggaactg aggactgaga cccgtgactg
      601 cagcaccgcc tgcgcttcga tctcgaaccg ggcgacgtcg aggagtgtcc ggccctcggc
      661 gatgtcggcg atgtcggcga tgatctgacc atcacccgcg agtcgaaggt tcgacaaccc
      721 gtggccccgt gccaaggtga tgagcgcctg agccaagcgc agcatctgag cttcgggagc
      781 agggtccatg gctctgacgg taccggccag gagggtgcct ttcacctgcg ttcgaccttg
      841 cccgcggatc ggagcgccca ccgcgccggt cacgtccggc gatacggtgc cgacgatgag
      901 cacctgcacc agctgcggcc gcgacgacga ggacacggtg gcggtgcgcc gtgtctacct
      961 ggtcctccct gacggggcgc cgacggaccc cggctcgatc gaggagctgg ccgaggccac
     1021 cgtcgtcgac gaggacgagg actggtgcgc cacctgtcgc gaccagtacc cgcacgtgct
     1081 ccggagctga cccggaaccg tcgccgacgc ggtacgaaca gctcgctgtc agcgcagcgc
     1141 tctcgggtcg accgcccgac cgctactggc cggcaccgcc cgccgccggc gctgccgtcg
     1201 tcgtcggtgc aaccgtcgtc gtcgaccggg gcaccgtggt gctcgacgcc cccggcgtgc
     1261 tcgacgacgt cgacgctccg cccggcgtgg tcgtggtcga cgcacgcggc tgcgtggtcg
     1321 acgcgccact cgacagcggc atgcccgagt taaggacctt cttgcccacg tcggcgggcg
     1381 ggaacttgca cgccgagtcg cccgcgatcg ccctgctcat gaaatcgcgc cagatccttg
     1441 ccgggaacgt cccgccggcc accgactggc ccttgtagga cttcatctgc ttcggctgct
     1501 cgtagcccat ccacaccgac gcggtcaggt tgcaggtgta gccggtgaac caggcgtcct
     1561 tggcgtcgtt ggtggtgccg gtcttgcccg cggcgggcgt ccgcagaccg gccgccgcgc
     1621 cggtcccctt gatgatcacg ccctggagca ccgtcgtgac ggtgtcggcc acctgttcgc
     1681 tgatcacctg ctgggggtgc acctcggtcg acgcgtcgaa gatgacgtca ccgtcggagg
     1741 actcgatccg ggtgatcacg tagggctcgg tgtgacgccc ccgatcggcg aacgtcgagt
     1801 acgccgaggc catgtcgagc accgacacct cctcggttcc cagcaccagc gcgtagacgg
     1861 ggtccagctt ggcccgcaca ccgaggcggt tcgccatctc ggccagccgg ggtggggtga
     1921 tggtgtcgac gatgcccgcg tacacggtgt tggtcgagtc ccaggtggcc tcttcgaccg
     1981 actgcacgcc gtggtcggcc ttgtcgtagt tgccgggctt ccacagcttg ccgggctcgg
     2041 cgtagacgtt ggggaacatc gtcgtgggcg gagcgcggaa ccgcgacttg accgagtagc
     2101 cctcctcgac gaacgcggcg agggcgaacg gcttgaaggt cgacccgggt gcccgccccg
     2161 acccgccgcc cgcgctgccc agcgccaggt tgaccttgtt ggtctcgtag tcgcgcccgc
     2221 cgtacatggc ccggacccga ccgtcggtgt cgatcgcgac cagcgacccc accggaccgt
     2281 cgggctggtt cagcgtcttg ttgaccgcgt cgaccgcgta cttctgcatg gccgggtcga
     2341 acgacgtgta gacccgcagg cccttggtct cggcccccgg cccgagccgc tcgcgcagtt
     2401 gcgagcgcac ccactccatc cagaactggg accccagctc cttgtacttc acgtcgccca
     2461 ggtcggtgtg ctcggccggt cggggccgga tggtcacctg ctggggcaga ccggtgctcg
     2521 tcttgtcctc ggtgttccac gggacctcgg cggcggcccg ggcgcgcgac tcggagatgt
     2581 agccctcctc gaccatggcg tcgagcaccg tctttcggcg ccgggtggcc tccttcgggt
     2641 cacgggttgc atccgcgtgc tcaggggcgc ggatcagacc ggccaggtag gcggcctgag
     2701 gcaggtccag gtcgcgggca cccacaccga agtaggcccg cgacgctgcc tcgacgccgt
     2761 aggccccccg cccgaagtag atctcgttga ggtagcgggt gaggatctcg cgcttgtcga
     2821 gggtctgctc gagcttgacc gccagcaccg cctccttgat cttgcgggtc agggtcttct
     2881 ccgagctcag gtagacggtc ttgacgtact gctgggtgat ggtcgagccg ccctgctgcg
     2941 acgcgctgtc gcccagcacg tccttggtga acgctcgggc gatgcccacc gggtcgacgc
     3001 cgttgtggtc gaagaacttg cggtcctccg ccgacagcac ggcctgcacc agcaccggcg
     3061 ggaggtcgtt gtaggtgacc acgacgcgct gctcgctggc cgacagccgg gccatcgcgt
     3121 tgtcgaaccc gcactggccg gcctggacct cgatgtcgca gacgaaggtg gtctcgtcct
     3181 gcggcttggc cggcggcagt tggatcgtgt tgagcaccgc ccacagcccg ccgaccgcca
     3241 cgacgaccag cacgaccacc gcatagccga cgcggcgcca ccgccacagg aaccgccggg
     3301 gagatcgcga cgtggtgtcg cccgcgaccg gtccggctcg acgggatgag ggcggtggtg
     3361 gggacggcat ggggacgcgg ggctgcccgg gaggtgcggg cggtcgtatc agtctgcctc
     3421 gcctggccgg tccgttcccc aacgaagctg agaggccccc cgggtgtcga ccggatgcga
     3481 tccttgcgtc agtccgagtc ccgccgaccg accggggtga tgtcgagcag gtggttccac
     3541 cgacactcca ggcagacctc gacggtgtag caacgcagtc caccgccccg gtggcggtcg
     3601 gccaacttga gcagctcggc tcggcccgcg accacccgac cgcccggcgg catccggggc
     3661 ccgaacacga accgggccag gcgcagctcg tgctccccgc acacggggca gtcgtggcgg
     3721 gcccgctggg cgcagttgcg accgacccgg agcagctcgt gctgggcgtc gcagacgtcg
     3781 gcccgggtca gctcgccggc ccggtagcgg tgcagcaggc gccggcgcgc gaagcggtag
     3841 tcgaccgagc ccggtgtggt cccgggacga ccgccggact ccggcgcgag gaacggcacg
     3901 gccgcccagg gtaccgcccg ctccggcggg aaggccctgc ggtcgtccgc tctccgggtc
     3961 gggagcggtc cgcggctgtg gccgcgtgct cggccagact gacgccgatg gctacctccg
     4021 cgtccgcgac cggtcgaccg cgccacccct cggcgggcaa cctgggcgcg cggcccgccc
     4081 tggtccgcga cgactcctca cctcaccgac tgcccgccgc cagcttcccc cacggcgccg
     4141 agaacggccg ggaccgggtc cgcctcggcc ccgacctcga cgacgacgcc ctcgatcggc
     4201 tgatcggacc ggtcgacggc aagcgggttc tcgacctggg ctgcggagcg ggggcggcca
     4261 ccatcgccct cgcccgtcgc ggcgcccgcg tcaccgccgt cgaggcgtcg accacccgcc
     4321 tggcccaggc ccgtgccgcc gccgaggtcg cggaggtccg cgtcgagttc caccacagcg
     4381 accttgccga cctggccttc gtccgagccg acaccgtcga cctggtcctg gcggtctact
     4441 cgttggccgg ggtccaggac ctctcacggg tcttccgcca ggtccaccgg gtgctcaccc
     4501 ccggcagcgc ccttctgctg tcgctgccgc acccgttcac caccatgctc gatgacgacg
     4561 ctgaccagcc gtcgccctac ctcacccgca cggcgtggag cgcatcgccg ctggcgtggc
     4621 gcgccgccgg cgacgagggc gtcacccacg tccaccagat cggcgacgtg ttcaccaccc
     4681 tgacgcgctc caacttcgcg gtcgacgctc tgctcgaacc cgaaccggtc gccggggtct
     4741 cgggcatcca cacctcgacg ctggtcgagt gggtgccgcc cacgctcgtg gtccgggccc
     4801 gcaagctcgg gatctgagcg ccgcgccgga tttctgaggt ccggattgcg acaaggtgtg
     4861 tcggtgcgga cccctatgtc caggcgtggg ctcgcgtcat cggcgagggt gacaggagca
     4921 gcttggtcca gtcgatcccc atgaggtcac cagcggtgac cacctcgaca ggaaccagcc
     4981 gatcaggatc gtccagggct cgatccatgc gagcgaacaa cgcgtcgacc acaccgggat
     5041 cgaactggga acccgtgcac cggacgatct cggcgcgggc acgcaagctg gtcatcggct
     5101 cgctgtacga gcgacaactg aggatggcgt cgaacgagtc cgccagcgtc acgatccgcg
     5161 ccaacggtga gatgcgctct ccggcgaggc cgaacggata cccatggccg tcccagcgtt
     5221 cgtgatgctg ggccacgcca tcgacggtcg cacccagcca cctccggtac ggatcgagca
     5281 tctgcatccc gatgagcgga tgagcccggg cgacgccgag ctcttcatcg ttgagacgac
     5341 cgtgcttgtt gaggatgccc ggaggaaccg cgaccttccc cacgtcatgg agggctgccg
     5401 cccagcgcag gtcgttcagc tcgtcgggat cgagtccgag ctccaagccg atctcgaccg
     5461 cgtacgctgt gacccgaccg gcgtgtatgg cggtcgagcg gtcgtggtca gccagcatcg
     5521 gcaggcagcg gagcgcgtgc tcatcgagcg actgcgcact cccgtcgact ctggacatgt
     5581 gagccccctc tccgaggtcc tccgccgtga tcgtgaccca tcaaccacgg cgccacatgg
     5641 gccacggggc ccgaatccca tgagtcggac gatccattga ggagagttgt ctctgctcac
     5701 ctcggatttc tgaggtccgg attgcgacaa ggtgtgtcgg tgcggacccc tatatct
//
LOCUS       DAOQOD010000189         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5607_length_5714_cov_8.962714, whole genome shotgun sequence.
ACCESSION   DAOQOD010000189 DAOQOD010000000
VERSION     DAOQOD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5607_length_5714_cov_8.962714"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLZ72_13920"
     CDS             complement(<1..275)
                     /locus_tag="PLZ72_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37442.1"
                     /translation="MSEPLPPEPLRGEPPVLTHDPASRVGPDASVRVVGAPVASTGDR
                     TTIDRGPVPALELDRAAPVPVAPAARRRHRRRWFSAAAVLAVLGAGCL"
     gene            complement(272..1768)
                     /locus_tag="PLZ72_13925"
     CDS             complement(272..1768)
                     /locus_tag="PLZ72_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNA37443.1"
                     /translation="MSAAAKAPEPEDFRALFDPAGVVVAGASTHPGKFGFVCLHNILA
                     SGYAGRVFATNRDGSPVLGHPTLRSLDELPEGAADLIFVCTPASANVELLRTAAAKGI
                     RAAFLASAGYGEAGEEGQRAQAELVALGAELGILIVGPNGQGVVSTPSKLCAQIVAPY
                     PPAGRIGVASQSGNFVSSFLNWSCATGVGISRAVSCGNAAATSVADFLRFFADDDATD
                     VALAYIEGVSDGPGVAAALTEVAARQPTVVLKGGATAGGARAAASHTGSLATDDRTFD
                     GLCRQAGITRAATVEEAFEAAATFATQPLPAGNRVVVLTTAGGWGVVTADAITAHRDL
                     ELATLPDDLRKRIDTLLPPRWSRNNPIDLAGGETRDTIPQVLELVAGHPDVDAVIYLG
                     LGIQSNQARMLRDGGFHPDHGIERIVAYHERQDARFAQAAHDVSVATGKPILTATELA
                     VAFPDNPGPATVRATGRVCYPSADRAVTALGHLWRRSRHLARRGVGTP"
     gene            complement(1765..2487)
                     /locus_tag="PLZ72_13930"
     CDS             complement(1765..2487)
                     /locus_tag="PLZ72_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNA37444.1"
                     /translation="MSQVDPRPPGADPARTRTLSESDSKDLLARFGIPFAPEHLVTDA
                     AGARAAADALGVPVAAKLCGTNIAHKSERGLVRLGLSGSDAVAAATDELLAAARPDDG
                     AAGVLIAPMLRGHRELIAGIATDEQFGPTVLLGIGGVLAEAVADVTVRLVPISRIDAE
                     EMIDDLRSQALLGEFRGEPALERDSVVDVLVGLSDAATAVPGLVSADLNPLIVSDGRA
                     VAVDALVELTEGTHLEPAEVRS"
     gene            2542..3699
                     /locus_tag="PLZ72_13935"
     CDS             2542..3699
                     /locus_tag="PLZ72_13935"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNA37445.1"
                     /translation="MTLVGAALSEHPLATHAVGETVGRLLELGGPRPDVVTLFATEPH
                     LGSLDDVVGAVRHLLEPRALVGASMQSVLAGSREVMDHAAVSLMAIWFGGTRVRPVRI
                     DSRPGSDGPELRGLPALDDASGTLVLLTDPFTFPSATAMGELARRAPALSVVGGAASA
                     ARRPGGNRLVLDGALHDHGAVGLWIDDTTPLATVVSQGCTPIGEPFTVTSAERTMVRE
                     LGGRPALERLLETVDGLDDDDRARAASGLLLGRVVDEMRETFGPGDFLVRGVLGADRD
                     AGALAVADEIPVGATVQFQVRDAPGATRDLVDSLGGISAAGALCLSDVARGSELFGAV
                     DHDATTVTDALDGAPVAGAFCAGTYGPVGGRVALHDSAVSMLLVDPRPTWG"
     gene            3775..>5714
                     /gene="tkt"
                     /locus_tag="PLZ72_13940"
     CDS             3775..>5714
                     /gene="tkt"
                     /locus_tag="PLZ72_13940"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNA37446.1"
                     /translation="MSQLPSPMDPALEQRAINVIRGLAMDAPAAARSGHQGTAMALAP
                     LAHVLWTRIMRYDASAPQWADRDRFILSCGHASILQYAMLHLTGYDVSIEDLESFRQW
                     GSRTPGHPEVGHTPGIEVTTGPLGQGFANGVGMAIAEASLRERFGVDVCDHHIWAMVS
                     DGDLSEGVSHEAASLAGHLGLGRLVYVYDDNHISIDGPTELALSDNAATRFDSYGWHV
                     IELGEAAEDLDAMEQALLEARSVEDRPSLVIVRSHIAFPSPRLTDDASAHGLAFGAED
                     ITETKSVMGLPDEPFFVPDDVRDLYRAAGARGRVAREDWEQRSRAALAGREADWEASI
                     GSRGLPGWADVLPAYTEADSPATRVASGDCVAALAGVVPGLVAGAADLIGNTGTKLPG
                     AGVLSAAEPGGRQIHFGIREHAMGSIMVGAAHHGGVLPAAGTFLVFSDYMRPAARLAA
                     LSGAKCVFVWTHDSVGVGEDGPTHQPVEHVMSLRLIPDLTVIRPADGNETAAAWRVAV
                     DGDGPVALILSRQNTPVLPTTATRAVDGVSRGGYVIADADAPDVVLVGTGTEVAVALE
                     AAGLLAEDGRKVQVVSLPCWDRFDAQDDDYRRSVLPPGVPTVSVEAGVTLGWDRYADA
                     SVGIDRFGASAPGGVVLRELGIT"
BASE COUNT          706 a         2087 c         2164 g          757 t
ORIGIN      
        1 aggcagccgg caccgagcac ggcgaggacc gccgcggccg agaaccagcg tcggcgatgc
       61 cgtcgccgcg ccgcgggcgc gaccggcacg ggtgccgccc ggtccagttc gagggcgggg
      121 accgggcccc gatcgatcgt cgtccggtcc ccggtcgatg cgaccggggc gccgaccacc
      181 ctcaccgacg catcgggtcc gacccgtgac gccgggtcgt gggtcagcac cggtggttcg
      241 ccccgcagcg gttcgggggg aagcggctcg ctcacggggt gccgacccca cggcgggcca
      301 ggtgacggct gcggcgccag aggtggccca gtgcggtgac ggcccgatcg gcggacgggt
      361 agcacacccg cccggtggcg cggaccgtcg ccggtcccgg gttgtcgggg aaggcgacgg
      421 ccagctcggt cgcggtgagg atcggcttgc cggtcgccac cgacacgtcg tgggcggcct
      481 gggcgaagcg ggcgtcctga cgctcgtggt aggcgacgat ccgctcgatg ccgtggtcgg
      541 ggtggaaccc cccgtcgcgc agcatcctgg cctggttgga ctggatcccg aggcccaggt
      601 agatgacggc gtcgacgtcg gggtgcccgg cgaccagctc gagcacctgt gggatggtgt
      661 cgcgggtctc gccgccggcc aggtcgatcg ggttgttgcg gctccagcga gggggaagga
      721 gggtgtcgat ccgcttccgg aggtcgtcgg gcagcgtggc cagctcgagg tcgcgatgcg
      781 cggtgatcgc gtccgcggtc accacccccc accctccggc ggtggtcagc acgacgaccc
      841 ggttgccggc gggcagcggc tgggtcgcga acgtcgctgc ggcctcgaag gcctcctcga
      901 cggtggcggc ccgggtgatg ccggcctggc ggcacaaccc gtcgaaggtg cggtcgtcgg
      961 tggcgagcga cccggtgtgg ctggcggcgg cccgcgctcc cccggcggtc gccccgccct
     1021 tgagcacgac ggtgggttgg cgcgccgcca cctcggtcag cgcggcggcg acgccggggc
     1081 cgtcggagac gccctcgatg taggccagtg cgacgtcggt ggcgtcgtcg tcggcgaaga
     1141 accgcaggaa gtcggcgacc gaggtggccg ccgcgttgcc gcacgacacg gcgcgggaga
     1201 tgccgacacc ggtggcgcag gaccagttga ggaagctgga cacgaagttg cccgactggc
     1261 tcgccacccc gatgcggccc gccggcgggt acggggccac gatctgcgcg cacagcttcg
     1321 acggggtcga caccacgccc tggccgttgg ggccgacgat caggatgccg agctcggcgc
     1381 cgagcgcgac cagctcggcc tgcgcccgtt ggccctcctc gccggcctcg ccgtagcccg
     1441 cggaggccag gaacgcggcc cggatcccct tggcggcggc ggtgcgcagc agctcgacgt
     1501 tggccgacgc cggcgtgcac acgaagatca gatccgcggc cccctcgggc agctcgtcga
     1561 gcgagcgcag cgtcggatgt ccgagcaccg gggacccgtc gcggttggtg gcgaagaccc
     1621 ggccggcgta cccgctggcg aggatgttgt gcaggcacac gaacccgaac ttgccggggt
     1681 gggtggaggc gccggcgacc accactcccg cggggtcgaa cagcgctcgg aagtcctccg
     1741 gctcgggtgc cttcgccgcc gcgctcacga tcggacctcc gccggctcga ggtgggtgcc
     1801 ctcggtcagc tcgaccaggg cgtcgacggc gacggcccga ccgtcggaca cgatgagggg
     1861 gttgaggtcc gcggacacga gccccggcac tgcggtggcg gcgtcggaca ggccgacgag
     1921 cacgtcgacg accgagtcgc gctccagcgc cggctccccc cggaactcgc cgagcagcgc
     1981 ctgcgatcgc aggtcgtcga tcatctcctc ggcgtcgatg cgcgagatcg gcaccaggcg
     2041 gaccgtgacg tcggcgacgg cctcggccag gacgccgccg atgccgagca ggaccgtcgg
     2101 gccgaactgc tcgtcggtgg cgatgccggc gatcagctcg cggtggcccc gcagcatcgg
     2161 ggcgatcagc acccccgcgg cgccgtcgtc gggtcgggcg gcggccagca gctcgtcggt
     2221 ggcggcggcg acggcgtcgg agccgctcaa cccgagacgc accagacccc gctcgctctt
     2281 gtgggcgatg ttggtgccgc agagcttggc cgccaccggc acccccaggg cgtcggccgc
     2341 ggcccgggcg ccggcggcgt cggtgacgag gtgctcggga gcgaagggga tcccgaagcg
     2401 ggccagcaga tccttggagt cggactccga cagggtccgg gtgcgggccg ggtcggcgcc
     2461 ggggggtcgg gggtcaacct gcgacatggg gtcgaaccct acgcggcccg caggtcccgg
     2521 tgcggtcgta gcatcgccgc gatgacgctc gtcggtgcgg ccctgagcga gcacccgctc
     2581 gccacccacg ccgtcggcga gaccgtgggc cggctgctcg agctcggagg tccccgaccc
     2641 gacgtcgtga ccctgttcgc gaccgagccg cacctcggtt cactcgacga cgtcgtcggc
     2701 gccgtgcgcc acctgctcga gccccgcgcg ctggtgggtg cgtcgatgca gtcggtgctg
     2761 gccggcagtc gggaggtgat ggaccacgcg gcggtgtcgc tgatggcgat ctggttcggc
     2821 ggaacccgcg tccgtccggt gcgcatcgac tcccggcccg gttcggacgg cccggagctg
     2881 agggggctgc cggcgctgga cgacgcctcc gggacgctgg tcctgctgac cgaccccttc
     2941 acgttcccgt cggccaccgc gatgggcgag ctggcgcgcc gggcacccgc cctgtcggtg
     3001 gtcggcggcg cagcctccgc ggcgcggcgt ccgggtggca accggctcgt gctcgacggg
     3061 gcgctccacg accacggagc ggtggggctg tggatcgacg acacgacgcc gttggcgacg
     3121 gtggtgtccc agggctgcac cccgatcggc gagccgttca ccgtgacctc ggcggagcgc
     3181 acgatggtgc gcgagctggg cggccgtccc gccctcgagc gcctcctcga gaccgtcgac
     3241 ggcctcgacg acgacgaccg cgcccgggcc gcgtccggtc tcctgctcgg acgggtggtc
     3301 gacgagatgc gcgagacgtt cgggccgggc gacttcctgg tgcgcggcgt gctcggcgcg
     3361 gaccgcgacg ccggggctct ggcggtcgcg gacgagatcc ccgtcggagc gaccgtgcag
     3421 ttccaggtgc gagacgctcc gggggcgacc cgggacctgg tcgactcgct cggcgggatc
     3481 agcgccgcgg gggcgttgtg cctgagcgac gtcgctcgcg gctcggagct gttcggcgct
     3541 gtcgaccacg acgcgaccac cgtcaccgac gccctcgacg gcgccccggt cgccggcgcg
     3601 ttctgcgccg ggacctacgg cccggtcggc ggccgcgtcg ctctgcacga ctcggcggtg
     3661 tcgatgctgc tcgtcgatcc ccgacccacc tgggggtgag ccgcaggggc cggattagac
     3721 ccgcccggga cgatcgctac gattcggccc gacaccgacc ggacaggact ccccatgtcg
     3781 cagctcccct ccccgatgga ccccgcgctc gagcagcgtg cgatcaacgt cattcgtggc
     3841 ctggcgatgg acgcccccgc cgcggcccgg agcggccacc agggaacggc catggcgctc
     3901 gccccgctgg cgcacgtgct gtggacccgg atcatgcgct acgacgcgtc ggcgccgcag
     3961 tgggccgacc gcgaccggtt catcctcagc tgcggtcacg cctcgatcct gcagtacgcg
     4021 atgctgcacc tgaccggcta cgacgtgtcg atcgaggacc tcgagtcgtt ccgccagtgg
     4081 gggtcgcgca cgccggggca ccccgaggtc ggccacaccc cgggaatcga ggtcaccacc
     4141 gggccgctcg gccagggctt cgccaacggc gtcggcatgg cgatcgccga ggcgtcgctg
     4201 cgggagcgct tcggcgtcga cgtctgcgac caccacatct gggcgatggt ctccgacggc
     4261 gacctgtccg agggcgtcag ccacgaggcc gcctcgttgg ccggacacct gggcctgggc
     4321 cgcctggtgt acgtctacga cgacaaccac atctcgatcg acggcccgac cgagctggcg
     4381 ctctcggaca acgccgcgac ccgcttcgac tcctacggct ggcacgtgat cgagctgggt
     4441 gaggcggccg aggacctcga cgccatggag caggccctgc tcgaggcccg ttccgtcgag
     4501 gaccggccgt cgctggtcat cgtgcgcagc cacatcgcgt tcccgtctcc gcgcctcacc
     4561 gacgacgcgt ccgcccacgg cctggccttc ggcgccgagg acatcaccga gaccaagtcg
     4621 gtgatggggc tgcccgacga gccgttcttc gtacccgacg acgtccggga cctctaccgc
     4681 gccgcggggg cgcggggccg ggtggcacgc gaggactggg agcagcgctc ccgcgccgcc
     4741 ctcgcgggtc gggaggccga ctgggaggcc tcgatcggtt ctcgagggct gccgggctgg
     4801 gccgacgtcc tgcccgccta caccgaggcc gactcccccg ccacccgtgt ggccagcggc
     4861 gactgcgtgg ctgcgctggc cggggtcgtc cccggactgg tcgccggtgc cgccgacctg
     4921 atcggcaaca ccggcaccaa gctccccggc gcgggggtgc tgtcggcggc cgagccgggc
     4981 ggacgccaga tccacttcgg catccgcgag cacgccatgg gatcgatcat ggtcggcgcc
     5041 gcgcaccacg gcggcgtgct gcccgcggcg ggcacgttcc tggtgttcag cgactacatg
     5101 cgccccgccg cccgtctggc cgcgctgtcg ggcgccaagt gcgtcttcgt gtggacccac
     5161 gactccgtcg gcgtcggcga ggacggcccc acccaccagc cggtcgaaca cgtgatgtcg
     5221 ttgcgactca tccccgacct gaccgtcatc cgcccggcgg acggcaacga gaccgccgcg
     5281 gcgtggcggg tcgccgtcga cggcgacgga ccggtcgccc tgatcctcag ccggcagaac
     5341 accccggtgc tgccgacgac ggcgacccgg gcggtcgacg gcgtgagccg cggcggctac
     5401 gtcatcgccg acgccgatgc ccccgacgtc gtgctggtcg gcaccggcac cgaggtggcc
     5461 gttgccctcg aggcggcggg actgctggcc gaggacggcc gcaaggtcca ggtcgtgtcg
     5521 cttccctgct gggaccgctt cgacgcccag gacgacgact accggcgctc cgtgctgccc
     5581 cctggggtgc cgaccgtgtc ggtcgaggcc ggtgtcaccc tcggctggga ccgctacgcc
     5641 gacgcgtcgg tcggcatcga ccgcttcggg gcgtccgccc ccggcggcgt ggtcctgcgc
     5701 gagctgggga tcac
//
LOCUS       DAOQOD010000190         5638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5735_length_5638_cov_12.955938, whole genome shotgun sequence.
ACCESSION   DAOQOD010000190 DAOQOD010000000
VERSION     DAOQOD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_5638_cov_12.955938"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLZ72_13945"
     CDS             complement(<1..797)
                     /locus_tag="PLZ72_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA37447.1"
                     /translation="MDDDASEVGAARDPGFLRAARPILERFASYFRPEVRGFDRLPEQ
                     GPMLVVGNHSGGQLPPDIPILLTAWWRERGEDEAVYALFHSFFLGLPGIGPLMARSGG
                     VEAGPATAEAILRRGDILIDYPGGDHEVFRPWGERNTIDFGGRKGVIRLALRTQVPIV
                     PAVSVGAHETLVVLSRGEGLARRLGLDKRFRVSVMPLVLGPPFGIVPGGIPTFPLPAK
                     VTVELLDPIDFSERFGPEAAEDPEVVDACFEELTSTMQDALDRLAAER"
     gene            complement(827..1126)
                     /locus_tag="PLZ72_13950"
     CDS             complement(827..1126)
                     /locus_tag="PLZ72_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37448.1"
                     /translation="MATTTTKTSTKSGTKDASENLQKFIDGLQANEKSALDAIKKFVD
                     TVNDVFPDFGEEGPRSKIIDAAFSMTQQIVESSNKMAKDLVGVTEDALDNIARSN"
     gene            1270..1713
                     /locus_tag="PLZ72_13955"
     CDS             1270..1713
                     /locus_tag="PLZ72_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNA37449.1"
                     /translation="MPDRVADNRRLLRARDAMDRDFSQPLDLAALASIACISESHFSR
                     SFKATFGETPHRYLQRRRIERAMFLLRTTDLTVTDVCMQCGFSSLGSFSRTFSDIVGE
                     SPSEFRSRGVVPVPVPTCFVRAWTRPSDTVPSRFGEATADGRSVR"
     gene            1718..2143
                     /locus_tag="PLZ72_13960"
     CDS             1718..2143
                     /locus_tag="PLZ72_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA37450.1"
                     /translation="MINSISRSQVFVLDQDEALDFYVSTLGFEVAADVDLGFMRWLTV
                     RVPGDEDREILLERPGPPSMDEATAEQVRELVSKGAIGGAIFLTTDDARSTFETLKGR
                     GVDIVDEPTERPYGIDFGIRDPFGNAIRIGQMFDTGLTW"
     gene            complement(2231..3211)
                     /locus_tag="PLZ72_13965"
     CDS             complement(2231..3211)
                     /locus_tag="PLZ72_13965"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNA37451.1"
                     /translation="MLREAMEGGSITTGTGRPTSSDARDRAVGALVGLAVGDAVGTAL
                     EFRAPGTFTPIDDMVGGGPFGLEPGQWTDDTSMAMCLAESILDIGELDLADQLRRYLA
                     WRDHGHWSSTGRCFDIGTTTSAALDHFQRTGRPVSTGPVDQDQAANGSLMRLAPVPIR
                     WHTDPAEAAERSGESSRSTHPADRPVDACRVYGAMIAALIGGNDIDEVLAPDFWDLGP
                     LHPQIEAVARGSWRSGDVRGTGYVVDALGAALWAVAGAVDFRDAVLRAANLGDDADTT
                     AAIAGQLAGARWGLAGMPEPWVGRITSGERIAAIAGRLFDAAAGAGSASP"
     gene            3329..3712
                     /locus_tag="PLZ72_13970"
     CDS             3329..3712
                     /locus_tag="PLZ72_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HNA37452.1"
                     /translation="MGVLAIDHVQLAMPPGVEAEARAEEFYSGALGLPRVPKPEPLAG
                     RGGCWFSNGVVALHLGVEQDFRPARKAHPALIVDDLAGLVTSLEAARVQVRPGEPLDG
                     YDRVHVDDPFGNRIELMERWDERSP"
     assembly_gap    3746..3845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3846..4852)
                     /locus_tag="PLZ72_13975"
     CDS             complement(<3846..4852)
                     /locus_tag="PLZ72_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37453.1"
                     /translation="MGRMRRLWAALAAVGASIAVVGGCSAPPATPQQQIDQIVQFVET
                     ARGHQFVTPPVVEFLSDDVFRSEVLASLDAAEPGVDADDVAFTALGWISPGESLWDEY
                     RIAFGGGVVGFYDPVAKELKVRGTDMTPYRREVIAHELTHALDDQLFDLSDSESEGLI
                     DEDHVAALVAIEGSASEVQRRYIDSMSDLERFQDLAEQLQFGSDPALLEVPVTLLTLT
                     SAPYLRGAIFQDQLIAALGNPAGPDLSLTRYPATTEQAFDTARFLADEPVVAVPLPPA
                     DGPVVRSGPFGQFMTTLLLREGLALDAVDPATVGWAGDAYVTWTDGTTDCLRLDTRMD
                     TA"
     gene            5155..5484
                     /locus_tag="PLZ72_13980"
     CDS             5155..5484
                     /locus_tag="PLZ72_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37454.1"
                     /translation="MSRTLAHRPERARFDDPSSCFPVHDHRDGPCDLPTLVEWHDWVN
                     HGDGEQPWRCEWELSWYLWPKLCSCRRCPDYYWNREDRRRDRHRSRLWLATGAWIEEW
                     VDRPVRH"
BASE COUNT          811 a         1923 c         1999 g          805 t
ORIGIN      
        1 cgctcggcgg ccagacggtc gagagcgtcc tgcatggtcg aggtcagctc ctcgaagcac
       61 gcgtcgacga cctcggggtc ctcggcggcc tcgggaccga agcgctcgga gaagtcaatg
      121 gggtcgagga gctcgacggt gaccttggcc ggcagcggga aggtcgggat gccgccgggg
      181 acgatcccga acggcgggcc gaggaccagc ggcatgaccg agacccggaa ccgcttgtcg
      241 agccctaggc gacgggccag accctcgccg cgggacagga ccaccagggt ttcgtgcgcg
      301 ccgaccgaca cggcggggac gatcggcacc tgggtccgca aggcgaggcg gatcacgcct
      361 ttgcgtccgc cgaagtcgat cgtgttgcgc tcaccccagg ggcggaacac ctcgtggtcg
      421 ccgccggggt agtcgatgag gatgtcgccg cggcgcagga tcgcctcagc cgtcgccggc
      481 ccggcctcca ccccaccgct gcgggccatg agtggaccga tcccgggcag gccgaggaag
      541 aacgagtgga acagggcgta gaccgcctcg tcttcgccgc gctcgcgcca ccaagcggtc
      601 agcaggatcg ggatgtcggg cggcaactgc cccccggagt ggttgcccac caccagcatg
      661 ggcccctgct ccgggaggcg gtcgaagccc cggacctcgg gacggaagta ggacgcgaag
      721 cgctcgagga tcgggcgggc tgcccggagg aagcccgggt cgcgtgccgc tccgacctct
      781 gacgcgtcgt cgtccacctc ccggtcgccg ctcggtccgg acccggtcag ttcgaccggg
      841 cgatgttgtc gagggcgtcc tcggtgacgc cgacgaggtc cttggccatc ttgttggacg
      901 actcgacgat ctgctgggtc atgctgaacg cggcgtcgat gatcttgctg cgggggcctt
      961 cctcgccgaa gtcggggaag acgtcgttga cggtgtcgac gaacttcttg atcgcgtcga
     1021 gggcggactt ctcgttggcc tggaggccgt cgatgaactt ctgcaggttc tcgctcgcgt
     1081 ccttggtgcc ggacttggtc gaggtcttgg tggtcgtcgt tgccatggtc ccctcctcgg
     1141 aggcccgggc gctgggggtg cacccgcgtg acgacgactc tgacatcccc ccgtcaccgg
     1201 gtccacggat tgggccgaga gtcacaccgc aaacctcgca ccggccgggg gccgcgacat
     1261 gctggcggga tgccggatcg tgtggcggac aacaggcgac tgctgcgagc gcgcgacgcc
     1321 atggaccgcg acttctccca gccgctcgac ctggccgcac tggcgtcgat cgcctgcatc
     1381 tcggagtcgc acttcagccg gtcgttcaag gcgacgttcg gcgagacccc gcaccgctac
     1441 ctccagcgtc gtcgcatcga acgcgcgatg ttcctgctgc gcaccaccga cctcaccgtc
     1501 accgacgtgt gcatgcagtg cgggttctcg agcctggggt cgttcagccg gaccttctcc
     1561 gacatcgtcg gcgagtcccc gagcgagttc cgcagtcgag gggtggtgcc cgtcccggtg
     1621 ccgacgtgtt tcgtgcgggc gtggacccga ccgtcagaca cggttccgag caggttcgga
     1681 gaagcgaccg ccgacggccg ttccgtacgg tgagcccatg atcaactcca tcagccgttc
     1741 ccaggtgttc gtcctcgacc aggacgaggc cctggacttc tacgtctcca ccctgggctt
     1801 cgaggtcgcc gccgacgtcg acctcgggtt catgcgctgg ctcaccgtgc gggtgccagg
     1861 cgacgaggac cgcgagatcc tcctcgaacg tccgggcccg ccgtcgatgg acgaggccac
     1921 cgccgagcag gtccgcgagc tggtcagcaa gggggccatc ggcggagcga tcttcctgac
     1981 caccgacgac gcccgctcca ccttcgagac cctcaagggg cggggggtcg acatcgtcga
     2041 cgagccgacc gagcggccct acggcatcga cttcggcatc cgggacccgt tcggcaacgc
     2101 catccggatc ggtcagatgt tcgacacggg actcacgtgg tgacacgggc gcgggcctga
     2161 gcgctggccc gcgtccgccc gggtcggtcg acgaccggcc cgggcggcgt cctcgacgct
     2221 ggccacgatc tcagggcgac gccgacccgg ctcccgcggc cgcgtcgaac agccgaccgg
     2281 cgatcgcggc gatgcgctca ccggaggtga tgcgaccgac ccagggctcc ggcatgcccg
     2341 ccaaccccca ccgggcaccg gcgagctggc cggcgatggc ggcggtggtg tctgcgtcgt
     2401 cgcccaggtt ggcggcccgc agcaccgcgt cgcggaagtc gaccgcgccg gcgaccgccc
     2461 acaacgcggc tccgagcgcg tcgacgacgt agccggtgcc gcgcacatcc ccgctccgcc
     2521 acgagccgcg ggcgacggcc tcgatctgcg gatggagcgg acccaggtcc cagaagtcgg
     2581 gggcgagcac ctcgtcgatg tcgttaccgc cgatgagggc ggcgatcatg gcgccgtaca
     2641 cgcggcaggc atcgactggt cggtcagcag gatgggtgct gcggctggac tcgccggacc
     2701 gttcggcggc ttcggccgga tcggtgtgcc agcggatggg gacgggcgcc aaccgcatca
     2761 gcgacccgtt ggccgcctgg tcctggtcga cggggccggt ggacactggc cggccagtgc
     2821 gctggaagtg gtccaacgcc gccgaggtgg tggtgccgat gtcgaagcac cggccggtcg
     2881 aggaccagtg gccgtggtcc cgccacgcca ggtaccgacg gagctggtcg gcgaggtcga
     2941 gctcaccgat gtcgaggatc gactcggcca ggcacatggc catggaggtg tcgtcggtcc
     3001 actgccctgg ctccagcccg aacggccctc cgccgaccat gtcgtcgatc ggggtgaagg
     3061 tgcccggagc acggaactcg agcgcggtgc cgaccgcgtc gccgaccgcc aggccgacca
     3121 gcgcgccgac cgcccggtcg cgcgcgtcgc tcgacgtcgg tcggcccgtc cccgtcgtga
     3181 tcgatccgcc ttccatcgct tccctcagca tccgtcgacc ccgggatagt ggcacgcccc
     3241 tgtgacgacg cggcgagcag ctccgactcg ggtccactcc gtcggccgac gcccgcacct
     3301 gtgcatcgcc cggtggcaca gtggggcgat gggtgtcttg gcgatcgacc acgtccagct
     3361 ggcgatgcca ccgggcgtcg aggccgaggc ccgggcggag gagttctact ccggggcgct
     3421 gggtctgcct cgggttccca aacccgaacc gctggccggc cggggcgggt gctggttctc
     3481 gaacggggtg gtggcgttgc acctcggcgt ggagcaggac ttccggccgg cgcgcaaggc
     3541 gcacccggcg ttgatcgtcg acgacctcgc cgggttggtg acgtcgttgg aggcggccag
     3601 agtccaggtc aggcctggcg agccgctcga cggctacgac cgcgtccacg tcgacgaccc
     3661 attcggcaac cgcatcgagc tgatggagcg gtgggacgag cgctcgccct gacctccgag
     3721 ggcggccacc cctacggccc tacagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnngcggt gtccatccgg gtgtcgaggc gcaggcagtc ggtggtgccg tcggtccacg
     3901 tgacataggc gtcaccggcc catccgacgg ttgccgggtc gacggcgtcg agcgccaacc
     3961 cctcccgcag cagcaacgtg gtcatgaact ggccgaacgg gcccgagcgg acgaccgggc
     4021 cgtcggcggg tggaagcgga accgccacga cgggctcgtc ggccaggaag cgggcggtgt
     4081 cgaaggcctg ctcagtggtc gccgggtagc gggtgagcga caggtccggg ccagcggggt
     4141 tgccgagcgc ggcgatgagc tggtcctgga agatcgcacc acggaggtac ggcgcgctcg
     4201 tgagggtcag caacgtgacc ggcacctcga gaagcgccgg gtcggagccg aactggagtt
     4261 gctcggcgag gtcctggaac cgctcgagat ccgacatcga gtcgatgtac cggcgttgca
     4321 cctccgaggc gctgccctcg atcgccacga gagcagcgac gtggtcctcg tcgatgagcc
     4381 cctcggactc ggagtcggac aggtcgaaga gctggtcatc gagcgcgtgg gtcagctcgt
     4441 gggcgatgac ctcccgccgg tatggggtca tgtcggtgcc ccggaccttg agctccttgg
     4501 cgaccgggtc gtagaagccg accactcccc cgccgaaggc gatccggtac tcgtcccaca
     4561 gcgactcgcc gggcgagatc cagccgagag cggtgaacgc gacatcatcc gcgtcgaccc
     4621 cgggctcggc ggcgtcgagc gacgcgagga cctcggagcg gaagacgtcg tcggagagga
     4681 actcgacgac cggcggcgtg acgaactggt ggccacgggc cgtctcgacg aactgcacga
     4741 tctggtcgat ctgctgctgc ggcgtggccg gcggtgcgct gcacccaccg accacggcga
     4801 tggacgctcc cacggccgcc aacgcagccc agagccgccg catccgtccc atgtcgcccc
     4861 ccggctcgac cgttgtcgct cggatggtag gacgaaccgg gtcccgccgg ccggggatcc
     4921 cgcaacgatc gaagtctctc cggcccaccg gatggctccg tcccctgggc ccgcgagggt
     4981 ccgaggctcg gctggcgtgc agtccacgga ggatctccgc ggccggaccg ggtgtcggac
     5041 gcggcagata ccctcggcgc catgacgagc tgtcgcgggc gcgcagcacg cccggcgtcc
     5101 cctccgtagc cggccaaccg gccggcggga cgccggggtg ctcgggagct gaccatgagc
     5161 cgcacccttg cgcaccgccc cgagcgggcc cggttcgacg atccgtcgag ctgcttcccc
     5221 gtccacgacc atcgtgatgg tccgtgcgac ctgccgacgc tcgtggagtg gcacgactgg
     5281 gtcaaccacg gcgacggcga gcagccctgg cggtgcgagt gggagctctc ctggtacttg
     5341 tggccgaagc tgtgcagctg tcggaggtgt ccggactact actggaaccg cgaggaccgc
     5401 cgccgcgacc gccaccgcag caggctgtgg ctggccacgg gcgcctggat cgaggagtgg
     5461 gtcgatcggc cggtccggca ctgagcgatc cccctgcgta gctcccggtg gggctcggcc
     5521 ccataggggt cccgccgccg gtggcttaca atccgcggag ctttccgcgg accaagacgc
     5581 ggaagtcccg cccgagtagc tcagctggca gagcagctgt cttgtaaaca gcaggtca
//
LOCUS       DAOQOD010000191         5572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5835_length_5572_cov_13.133587, whole genome shotgun sequence.
ACCESSION   DAOQOD010000191 DAOQOD010000000
VERSION     DAOQOD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_5572_cov_13.133587"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..703)
                     /locus_tag="PLZ72_13985"
     CDS             complement(65..703)
                     /locus_tag="PLZ72_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37455.1"
                     /translation="MTSTPTPGTASTAPATVRTRHRRGPLVAIAVVLALFGAACTPSP
                     DDPGSIDIPFGPITVPLPPIGAVPPPVTVDLILCKVGYVPPAITIRNATVTIPGLRIN
                     PNTTEVGVPNVSVNLPQNNLHLPGAYLSCLGLGGATEVALVIPAQVHVRSAMLNLQTG
                     VLSLNNPTITVTGVGVNLFGLGLIVPMPPFPIQLPTITVPLAGLAGGGAAAG"
     gene            complement(728..1279)
                     /locus_tag="PLZ72_13990"
     CDS             complement(728..1279)
                     /locus_tag="PLZ72_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37456.1"
                     /translation="MTIEATTADPSNRPGRYRRRGPLVALAVVIATVAAACSPTPNGF
                     DIALGGTIPLPTIQTDTTPVIVGDVLGCQLGYTPPGVSIVGASATVPGVNVEWTGVIT
                     VPNVKVNLPAITAVLPALSICGNTGAGIPINLPPLSVVATGQLNLATSQLVLKGVVIS
                     THIDLFGMVIPIDIPLDTITVQL"
     gene            complement(1457..2089)
                     /locus_tag="PLZ72_13995"
     CDS             complement(1457..2089)
                     /locus_tag="PLZ72_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA37457.1"
                     /translation="MTITADSSPGAAAAERPPSKGAQTRQAVLAAAIERFGRDGFRAT
                     SVADIARDAGVGGTVAYSYFPNKEALFLAAVDEDAAAVIDAGFLRVFNDPDLDDWQTQ
                     LIVTLVDAVDHHPLARRLLAGLEPEVTARVLDIPALEDVRRVCADRLRRDQGTGEVRP
                     DIDPVRVADGLVSIVLLVLMSVVQLGPAITAEHGPDVLAVIRAAIDPVRS"
     gene            2164..3117
                     /locus_tag="PLZ72_14000"
     CDS             2164..3117
                     /locus_tag="PLZ72_14000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNA37458.1"
                     /translation="MTDLMDRTASGPTAVDSTAPELDRGADFAPVPARRIPTRRISFE
                     ARLRNVRKYFAADDDLLNSHLAVALSSVFPDGEDFFVRSVRHYRSRITDPELRKQVAG
                     FIGQEATHGREHREFNDRLAELGYPTKENEWITRKGLELRSRIAPASSNLAATAALEH
                     FTATMAEVLLTDGTLHELFGDDAVRDLFLWHALEECEHKAVAFDVYKAIGGGERMRVW
                     TMVGLRYGFVVGMAVSMALAVAGDRNAYEKGRLRASWKRFKRNPLLQRSVWQRLKDYD
                     RPDFHPDDHDTDALVAEWRERLFGDAGELNDQLASRPAERV"
     gene            3171..4721
                     /locus_tag="PLZ72_14005"
     CDS             3171..4721
                     /locus_tag="PLZ72_14005"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37459.1"
                     /translation="MTDTTQADARSADGAVEHLDVLIVGAGLSGIGAAHYVSTRCRWA
                     TYAIFEARDSIGGTWDLFRYPGIRSDSDMFTFSYSFRPWQGAKAIADGDTILQYLKDT
                     AAEDGTDRHIRFGHRIVRAEWSTDDARWTVTAERTDTGEQVVLTCSFLFSCTGYYRYD
                     RGYVPDFDGLDDYAGEFIQPQLWPEDLDVAGKRVVVIGSGATAVTLVPSLARLGARVT
                     MLQRSPTYVVALPNRNPIADLARKVLPVSISGPMLRWMMVTGTVGLYQLSRRRPGLVK
                     KVLRRRLERQLPAGYDIDTHFTPSYDPWDQRLCVVSNGDMFAAIAEGTAEVVTDHVDR
                     FTPTGIRLRSGAELDADVVVAATGLDLLFMGGMDVIVDGDKVDPADRVTYKGMMLDGV
                     PNAALAVGYTNASWTLRSDLISDYVCRLLEHMRRSGMRQCTPTPGDDLTERAPLLGLS
                     SGYITRTVDRFPKQGTRAPWKVAQNYFYDYRQMRRSDVVDAEMVFSNPRSASAPVAAR
                     SAAEEIDA"
     gene            4727..5572
                     /locus_tag="PLZ72_14010"
     CDS             4727..5572
                     /locus_tag="PLZ72_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA37460.1"
                     /translation="MQGRVAAITGAGSGIGQALAVELARRGTHLSLTDIDTDGLARTV
                     ADCEGRGVKVTSRRLDVADRDAIYEWADSVPAEHGHINMLFNNAGVALVADVATMSDE
                     DLHWLVDINLWGVVNGCRAFLPHLEDSGQGHIVNVSSVFGLISIPSQSAYNIAKFGVR
                     GFSDALRMELEIARSCVSVTTVHPGGVKTNIARRARQDPATAAPVKPGQDLGDAFDRI
                     ARTTPRGAAAQILTGVEHDRRRITVGPDGRAIDLISRLPAGFYQRVLVTGSRLDRRFR
                     SGRGA"
BASE COUNT          834 a         1933 c         1998 g          807 t
ORIGIN      
        1 gcgggctgcg gcgcgctggg ccgagcagct cgccgcggag tccgcgggct gagccccgag
       61 gagatcagcc cgccgcggct ccgccgccgg cgagcccggc caacgggacc gtgatggtcg
      121 gcagctggat cgggaacggc ggcatcggga cgatgaggcc gagtccgaac aggttcacgc
      181 cgactccggt gacggtgatc gtcgggttgt tgagcgacag cacgccggtc tggaggttca
      241 gcatggccga gcggacgtgc acctgggccg ggatgaccag ggcgacctcg gtcgcaccgc
      301 ccagtcccag gcacgacagg taggcgccgg gcaggtgcag gttgttctgc ggcaggttga
      361 ccgagacgtt cgggaccccg acctcggtgg tgttggggtt gatccgcagg ccggggatgg
      421 tgacggtggc gttgcggatg gtgatcgccg gcggcacgta gcccaccttg cacaggatca
      481 ggtccaccgt caccggcggg ggaaccgccc cgatcggggg cagcggcacc gtgatgggcc
      541 cgaacgggat gtcgatcgag ccggggtcgt cgggtgacgg ggtgcacgcc gcgccgaaca
      601 gggcgaggac gacggcgatg gcgacgagcg ggccgcggcg gtgccgggtc cgaaccgtgg
      661 ccggtgcagt gctggcggtc ccgggggtcg gggtcgatgt catggttcga tttctcctgc
      721 gccgcgacta cagctggacc gtgatcgtgt cgagcgggat gtcgatcggg atgaccatgc
      781 cgaacaggtc gatgtgggtg gagatcacca ctcccttcaa cacgagctgc gaagtggcca
      841 ggttgagctg gcccgtggcg accacgctga gcgggggcag gttgatcggg atgccggcac
      901 cggtgttgcc gcagatcgac agggcgggca gcaccgcggt gatcgccggc aggttgacct
      961 tgacgttcgg cacggtgatc acgccggtcc actcgacgtt gacgccgggc acggtcgccg
     1021 aggctccgac gatcgagacg cccggcggcg tgtagccgag ctggcagccg aggacgtcgc
     1081 cgacgatgac cggggtggtg tcggtctgga tggtgggcag cgggatcgtg ccccccagcg
     1141 cgatgtcgaa gccgttcggc gtgggggagc aggccgccgc gacggtggcg atcacgacgg
     1201 cgagggccac gagcgggccg cggcgccggt accgaccggg acggttcgac gggtcggcgg
     1261 tggtggcttc gatggtcatg gttccctccc gtggatcggg cccgcagcgc ccggtcgtgc
     1321 gcccggagcg tggcgtggtg accacctccg gtgaccgacg tcacgccggt ccgcccccca
     1381 cctttacccc atggccaccg tcgatgccag cgttaccgat ccgtgccctc gtgctcgggt
     1441 ccgggtgggc cgtggttcag ctccggacgg ggtcgatggc cgcccggatg acggcgagga
     1501 cgtccgggcc gtgctcggcg gtgatggcgg ggccgagctg cacgaccgac atgagcacca
     1561 acaacacgat gctgaccagt ccgtcggcca cccgcaccgg gtcgatgtcg ggccggacct
     1621 ctccggtgcc ctggtcccgt cgcaggcggt cggcgcacac gcggcgcacg tcctcgaggg
     1681 ccgggatgtc gaggacgcgg gcggtgacct cgggttcgag tccggccagc agacgtcgcg
     1741 ccagggggtg gtggtcgacc gcgtcgacca gggtgacgat cagctgcgtc tgccagtcgt
     1801 cgaggtcggg gtcgttgaag acgcgcagga agccggcgtc gatgaccgct gcggcgtcct
     1861 cgtcgaccgc ggccaggaag agggcttcct tgttggggaa gtacgagtag gcgacggtgc
     1921 cgccgacccc ggcgtcgcgg gcgatgtcgg cgaccgaggt ggcgcggaac ccgtcacggc
     1981 cgaaccgctc gatggccgcg gccaggacgg cctggcgggt ctgggcgccc ttgctcgggg
     2041 ggcgctccgc cgcggccgca ccgggtgagc tgtcggcggt gatcgtcacg cccgccagtg
     2101 taggccagat cgcagaaaaa tgagggattg agtaaatcac tcagatcgtc tacagtcgcc
     2161 accgtgaccg acctcatgga ccgaaccgca tccgggccga ctgctgtcga ctcgaccgca
     2221 ccggagctcg acaggggtgc tgacttcgcc cccgtgcccg cccgccgcat cccgacccgg
     2281 cgcatctcct tcgaggcccg gctgcgcaac gtgcgcaagt acttcgcggc cgacgacgac
     2341 ctgctcaaca gccacctggc cgtcgccctg tcgtcggtct tccccgacgg cgaggacttc
     2401 ttcgtccggt cggtgcgcca ctaccgctca cgcatcaccg acccggagct gcgcaagcag
     2461 gtggccggct tcatcggcca ggaggccacc cacgggcgcg agcaccggga gttcaacgat
     2521 cgcctggccg agctcgggta tcccaccaag gagaacgagt ggatcacccg caagggtctg
     2581 gagctgcggt cgcggatcgc gccggcgtcg tcgaacttgg cggccaccgc tgcgctcgag
     2641 cacttcaccg ccaccatggc cgaggtgctg ctcaccgacg gcacgctcca cgagctcttc
     2701 ggtgacgacg cggtgcgcga cctgttcctg tggcacgcgc tggaggagtg cgagcacaag
     2761 gccgtcgcat tcgacgtcta caaggcgatc ggcggcggcg agcgcatgcg ggtgtggacc
     2821 atggtcggtc tgcgctacgg gttcgtcgtc ggcatggcgg tgtcgatggc gctggcggtg
     2881 gccggtgacc gcaacgccta cgagaagggt cgcctgcggg ccagctggaa gcggttcaag
     2941 cgcaacccgc tcctgcagcg ctcggtgtgg caacgtctca aggactacga ccgaccggac
     3001 ttccaccccg acgaccacga caccgacgcc ctggtcgccg agtggcgcga gcgactcttc
     3061 ggggacgccg gcgagctcaa cgaccagctg gcgtcccgcc cggccgagcg ggtctgagac
     3121 caccgaccga tccggacccg tccggcaccg attcgaacga ggctgacgac atgaccgaca
     3181 ccacgcaggc cgacgccagg tccgccgacg gcgccgtcga gcacctcgac gtcctcatcg
     3241 tcggcgccgg gctctccggg atcggggccg cccactacgt gtcgacccgg tgccggtggg
     3301 cgacctacgc gatcttcgag gcgcgggact cgatcggtgg gacctgggac ctgttccgct
     3361 acccggggat ccgctcggac tccgacatgt tcaccttcag ctactcgttc cggccgtggc
     3421 agggggccaa ggcgatcgcc gacggcgaca cgatcctgca gtacctgaag gacaccgcgg
     3481 ccgaggacgg gaccgaccgc cacatccgct tcggtcaccg catcgtccgg gccgagtgga
     3541 gcaccgatga cgcacgctgg accgtcaccg cggagcgcac cgacaccggc gagcaggtcg
     3601 tcctcacctg ttcgttcctg ttctcctgca ccggctacta ccgctacgac cgcggttacg
     3661 tccccgactt cgacggcctc gacgactacg ccggcgagtt catccaaccg cagctgtggc
     3721 ccgaggacct cgacgtcgcc ggcaagcggg tcgtggtgat cggcagcggc gccaccgcgg
     3781 tgaccctggt gccgtcgctc gcccggctcg gggccagggt gaccatgctg cagagatcgc
     3841 cgacctacgt ggtggcgctc cccaaccgga acccgatcgc cgacctggcc cgcaaggtgc
     3901 tcccggtgtc gatctcgggt ccgatgctgc ggtggatgat ggtgacggga accgtcggct
     3961 tgtaccagct gagccgccgt cgacccgggc tggtcaagaa ggtcctgcgg cgtcgactcg
     4021 aacgccagct cccggcgggc tacgacatcg acacccactt cacgcccagc tacgacccgt
     4081 gggaccagcg cctgtgcgtc gtgtccaacg gcgacatgtt cgccgcgatc gccgagggca
     4141 ccgccgaggt cgtgaccgac cacgtcgatc gcttcacccc gaccggaatc cggctgcggt
     4201 ccggcgccga actcgacgcc gacgtcgtgg tggccgccac cggcctggac ctgctgttca
     4261 tgggcggcat ggacgtgatc gtcgacggcg acaaggtcga cccggccgat cgggtcacct
     4321 acaagggcat gatgctcgac ggcgtcccga acgccgcgct ggccgtcggc tacaccaacg
     4381 cctcgtggac gctgcgctcg gacctgatca gcgactacgt gtgtcggctg ctcgagcaca
     4441 tgcgccggtc ggggatgcgt cagtgcacgc ccacgcccgg cgatgacctc acggaacggg
     4501 caccgctgct cgggctgagc tcgggctaca tcacccggac ggtggaccgg ttccccaagc
     4561 agggcaccag ggctccgtgg aaggtcgcac agaactactt ctacgactac cggcagatgc
     4621 ggcgcagcga cgtggtcgac gccgagatgg tgttctccaa cccgcggtcc gcgtcggcgc
     4681 ccgtcgcggc ccgctccgct gccgaggaga tcgacgcgtg aaggacatgc agggccgcgt
     4741 ggcggccatc accggggcgg gatcgggcat cggccaggcc ctggccgtcg agctggcccg
     4801 tcggggcacg cacctgtcgc tgaccgacat cgacaccgac gggctggccc gcaccgtcgc
     4861 cgactgcgag ggccgcggcg tcaaggtgac ctcgcgtcgg ctcgacgtcg cggaccgaga
     4921 cgccatctac gagtgggccg actcggtgcc cgccgagcac ggccacatca acatgctgtt
     4981 caacaacgcg ggggtcgccc tggtcgccga cgtcgcgacg atgtccgacg aggacctcca
     5041 ctggctggtc gacatcaacc tgtggggcgt ggtcaacggg tgccgggcgt tcctccctca
     5101 cctcgaggac tccggccagg gtcacatcgt caacgtctcg agcgtgttcg ggctgatcag
     5161 catcccgtcg cagtccgcgt acaacatcgc caagttcggc gtgcgcgggt tctccgacgc
     5221 cctgcgcatg gagctcgaga tcgcccgcag ctgcgtgtcg gtcaccacgg tgcaccccgg
     5281 cggggtcaag accaacatcg cccgccgcgc ccggcaggac ccggcgaccg cggccccggt
     5341 caagcccggc caggacctcg gcgacgcctt cgaccgcatc gcccggacca cgccgcgcgg
     5401 tgccgcggcg cagatcctga ccggcgtcga acacgaccgg cgacgcatca ccgtcggacc
     5461 ggacggtcgg gcgatcgacc tcatctcccg ccttcccgcc gggttctacc agcgggtcct
     5521 ggtgaccggg tcccggctcg accgtcggtt ccgatccggc cgcggcgcct ag
//
LOCUS       DAOQOD010000192         5491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5966_length_5491_cov_12.491722, whole genome shotgun sequence.
ACCESSION   DAOQOD010000192 DAOQOD010000000
VERSION     DAOQOD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_5491_cov_12.491722"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..652
                     /locus_tag="PLZ72_14015"
     CDS             92..652
                     /locus_tag="PLZ72_14015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNA37461.1"
                     /translation="MSAPSETGSAWSILPLDPDDAPTIDEVLALNEHWVPHVGSIGRD
                     ALRALVEAADLAVVAVPSGSGDGGAVEGFLILIAPGADYASPNYRYFQDRLDTGAAPG
                     PFRYVDRIAVSPRAQGRGLGRVLYDAAVERAVAVGAAEVTCEVNLEPPNPDSLAFHDR
                     LGFVPVGTQWTYDGTVRVQLMARRCG"
     gene            complement(667..1755)
                     /locus_tag="PLZ72_14020"
     CDS             complement(667..1755)
                     /locus_tag="PLZ72_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNA37462.1"
                     /translation="MPAPHPPSSAPTSVPSSGANLPRAGTNPTRLLSLDVTRGLAVAA
                     MLAVNNEGINPGHPGWLRHPEWNGFSVADLVFPLFLFCIGVSSTLSTRQRPGPGRSFM
                     DAFGPMARRCAILFALGVGLSALKQHQLVFAGVLQHIAVTSLLAWFVLLLPRRAQYAV
                     GVTILVAAAGFGLAWGFADGHTLDQAVDRFLYGRETAEGLPVAVVSVFNVLVGAWVGR
                     WFRTGDHRRIVRSLTTWTLGALAVGVLLVPWIPINKRLWTPSYALVGAAGSAAITLAM
                     YWLVDRRAIRRPLEWLRELGANPLAVYVACSALAALVPDRVRLAVVRAMADVVPLAVA
                     SVLWSVLWIVLAWVIADQLWRRRILIKL"
     gene            1899..2714
                     /gene="aqpZ"
                     /locus_tag="PLZ72_14025"
     CDS             1899..2714
                     /gene="aqpZ"
                     /locus_tag="PLZ72_14025"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNA37463.1"
                     /translation="MDDQIGKKLGAEFIGTFLLVFGGCGSAIFAAAALGATKGILGIG
                     GAELPAAGVVNYGIGYLGVALAFGLTVVCGAYGLGHVSGGHFNPAVSVGLATAKRFAW
                     KDVPAYVVTQCLAAIAAVGVLWAIQAGRPGGFTIRQGSFASNAYGQENGRIFFDLPAA
                     FIAELVLTALFVIVILGVTTKAASKAQAALGIGLMLTLIHLISIPITNTSVNPARSLG
                     PALFERGTALGQLWLFIVAPVVGGALGAVVWKLATGGDETQTGESEAQGVELT"
     gene            2854..3768
                     /locus_tag="PLZ72_14030"
     CDS             2854..3768
                     /locus_tag="PLZ72_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37464.1"
                     /translation="MGMLDGKVAIVTGAGHGIGRGHAMELAKHGATVIVNDLGGSVTG
                     EGAGRDADLTVDIIKARGGQAAPNYADVSDNDQAGEMIAQAVNDFGRLDILVNNAGIV
                     RDGSIFNMSVDDFDAVLKVHLRGTWIPCKHAALHWRALNKDTGQKVNGRIINTVSGAG
                     LTGNFGQSNYAPAKAAIASLTQTLSLELYRMGVTVNAVGPAAATRITGTMPNAPAVIE
                     ADEIPEDEWNRMDPAVSSPLVAWLASDEADHVTGQIIRAVAENIILMKGWADGPTINN
                     KGQRWDATKLGQQLATDVFFTRAPGLRY"
     gene            3840..4652
                     /locus_tag="PLZ72_14035"
     CDS             3840..4652
                     /locus_tag="PLZ72_14035"
                     /inference="COORDINATES: protein motif:HMM:NF022740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HNA37465.1"
                     /translation="MTRDILDEPGPARFAESYPSVGAAIGLAVLHRSTGLRGRIDKFT
                     GDIVQIRDPAGREHRFRNRPGAFAVAGETVTLVRPAPPPTTATGTLRRTAAGALVSVE
                     HTARVAQPSRLWVEGSHDARLLERVWGDDLRDLGIVVEPMGGIDDLVAAVAEFDPRPD
                     RRLAVLVDHLVPGSKETRLAQQVEGPNVLVVGHRYVDVWQCVRPVALGIDAWPEVPRG
                     EEWKAGVCARLGWGDPQDGWRRVLAAVDTFADLEPDLVGAVERALDVLAAES"
     gene            4661..>5491
                     /locus_tag="PLZ72_14040"
     CDS             4661..>5491
                     /locus_tag="PLZ72_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37466.1"
                     /translation="MTDGRTVEDLLASRLRRLGVRRTYGSALGDLDHVPVDDPDLAVL
                     LADADGRIGHVDGSGRLGAAALAGPILHLSSSPGGTAPLQTIGSAEELVEALADPPGL
                     SVPGTLALHLDLDLHEPLDAEPVPESGGDRSVVLLLDPSLAGMRILVLAGPGVVRTYS
                     HVGLRDLSRAAGAPVLATFGAIGLERWDSPYHAGVGGLQARDLHLGGLDDADLVITTG
                     LDPAELPSGRLDRLVVQDVAPRQLGALLRDWPARGPEPQDRPSVRGALGPVLAPLWEA
                     D"
BASE COUNT          807 a         1984 c         1958 g          742 t
ORIGIN      
        1 accggcaagg tgctcaagca cgtgctgcgc gacgagttca agggctgaga ccgggggttc
       61 cgcacggtgc aggccgtgag aggctgagcc gatgtccgcc ccctccgaga ccggttccgc
      121 ctggtcgatc ctgccgttgg accccgacga cgccccgacg atcgacgaag tcctcgcctt
      181 gaacgagcac tgggtccccc acgtgggctc catcggtcgg gacgccctgc gggccctggt
      241 cgaagcggcg gacttggcgg tggtggcagt cccctcgggt tccggtgacg ggggtgctgt
      301 cgaggggttc ctgatcctga tcgccccggg cgccgactac gcctcaccca actaccggta
      361 cttccaggac cggctcgaca ccggggccgc acccggcccg ttccgctacg tggaccgcat
      421 cgccgtgtcg ccccgggccc agggccgtgg gctgggtcgg gtgctctacg acgccgccgt
      481 cgagcgggcg gtcgcggtcg gggcggcgga ggtgacctgc gaggtgaacc tggagccgcc
      541 gaaccccgac tcgctggcgt tccacgatcg gttgggcttc gtcccggtcg ggacccagtg
      601 gacctacgac gggaccgtcc gggtgcagct gatggcccgg cggtgcgggt gaggccgtcg
      661 ggccggctac agcttgatga gaattcggcg gcgccacagc tgatcggcga tcacccaggc
      721 cagcacgatc cacagaaccg accagagcac cgacgccacc gcgagcggca cgacgtcggc
      781 catggctctg accacagcca gcctcacccg gtccgggacg agggcggcca gggcgctgca
      841 ggcgacgtag accgccagcg ggttcgcccc cagttcgcgg agccactcga gcggacgacg
      901 gatcgcccgt cgatccacca gccagtacat ggcgagcgtg atcgccgccg atccggccgc
      961 tccgacgagc gcgtagctcg gcgtccagag ccgcttgttg atcgggatcc aggggacgag
     1021 cagcactccg acggccaggg cgccgagggt ccacgtcgtc agactgcgca cgatccgacg
     1081 gtggtcgccg gtccggaacc agcggccgac ccatgccccg accagcacgt tgaacaccga
     1141 gacgaccgcg accggcagac cctcggcggt ctcgcgtccg tacaggaacc ggtcgacggc
     1201 ctggtcgagg gtgtgcccgt cggcgaaccc ccacgccagg ccgaacccgg cggccgcgac
     1261 caggatggtc accccgaccg cgtactgggc gcgccggggc agcagcagga cgaaccacgc
     1321 cagcagtgac gtcaccgcga tgtgctgcag gacgccggcg aacacgagct ggtgctgctt
     1381 gagggccgac agcccgacgc cgagggcgaa caggatggcg cagcgtctgg ccatcggtcc
     1441 gaacgcgtcc atgaaggacc gcccgggccc cggtcgctgc cgggtcgaca gcgtgctcga
     1501 caccccgatg cagaacagga acagcgggaa gaccagatcc gcgaccgaga acccgttcca
     1561 ctcggggtgg cgcagccagc cgggatggcc ggggttgatg ccctcgttgt tcaccgccag
     1621 catcgcggcc accgcgagtc cccgcgtgac gtcgagggag agcagccgcg tggggttcgt
     1681 gccggcccga ggcagattcg ctccggacga cgggacggat gtcggtgctg acgacggcgg
     1741 gtgcggcgcg ggcacccggc cacggtagga cggggtcggg gcgcgagcgg ggcgcgggtc
     1801 cccggtccgc ggcgtggtca cagctaccgt gcgcggtgct gggacatcgg aacgatcgtg
     1861 gctccagcgg gggcggcgtc aagcgaagga gtccttgcgt ggacgaccag atcggcaaga
     1921 agctcggcgc cgagttcatc ggcaccttcc tgttggtgtt cggcggctgc ggatcggcga
     1981 tcttcgccgc cgcggcgctc ggagccacca agggcatcct cggcatcggc ggcgccgagc
     2041 tgcccgcggc gggggttgtc aactacggca tcggctacct gggcgtcgcc ctggccttcg
     2101 gtctcaccgt cgtgtgcggc gcctacgggc tcggccacgt gtccggcggt catttcaacc
     2161 cggcggtgtc ggtcggcctc gccaccgcca agcggttcgc ctggaaggac gtgccggcct
     2221 acgtcgtgac ccagtgcctc gcggcgatcg ccgcggtcgg ggtcctgtgg gcgatccagg
     2281 ccggccggcc cggcgggttc accatccgcc agggctcgtt cgcctcgaac gcctacggcc
     2341 aggagaacgg ccgcatcttc ttcgacctgc ccgctgcgtt catcgccgag ctggtgctga
     2401 ccgccctgtt cgtcatcgtg atcctgggcg tcaccaccaa ggcggcgtcc aaggcgcagg
     2461 ccgccctcgg catcgggctc atgctgacgc tgatccacct gatcagcatc ccgatcacca
     2521 acacgagcgt gaacccggcc cgctcgctcg gcccggcgct gttcgagcgg ggcacggcgc
     2581 tcggccagct gtggctgttc atcgtcgccc ccgtcgtcgg cggcgccctc ggtgcggtgg
     2641 tgtggaagct cgccacgggc ggcgacgaga cccagaccgg cgagtccgag gcccagggcg
     2701 tcgagctcac ctgacccccc cacccgttct gttcgttgtt tcacaccccc agggttgtga
     2761 aacaacgaac agaacggggg tttgggggga ggtgtgcggg ttcctctagc atctgacgac
     2821 ccgtcaggtc accgagaagg ggagtcaccc accatgggca tgttggacgg caaggtcgcg
     2881 atcgtcaccg gagcggggca cggcatcggc cgcggccacg cgatggagct ggccaagcac
     2941 ggcgcgaccg tcatcgtcaa cgacctcggc ggatcggtga ccggcgaggg tgcgggccgc
     3001 gacgcggacc tgaccgtcga catcatcaag gcccggggcg gtcaggcggc gcccaactac
     3061 gcggacgtgt ccgacaacga ccaggccggc gagatgatcg cccaggccgt caacgacttc
     3121 ggacgactgg acatcctggt caacaacgcc ggcatcgtcc gcgacggctc gatcttcaac
     3181 atgtcggtcg acgacttcga cgccgtgctc aaggtgcacc tgcgcggcac gtggatcccg
     3241 tgcaagcacg cggcgctgca ctggcgggca ctcaacaagg acaccggcca gaaggtgaac
     3301 ggccggatca tcaacacggt ctcgggtgcg gggctgaccg gcaacttcgg ccagtcgaac
     3361 tacgccccgg ccaaggcggc catcgccagc ctcacccaga cgctcagcct cgagctctac
     3421 aggatgggcg tcaccgtcaa cgccgtcggt ccggccgccg cgacccgcat caccggcacc
     3481 atgcccaacg ccccggcggt catcgaggcc gacgagatcc ccgaggacga gtggaaccgc
     3541 atggacccgg cggtgtcctc gccgctggtc gcctggcttg cctccgacga ggccgaccac
     3601 gtcaccggcc agatcatccg ggcggtggcc gagaacatca tcctgatgaa gggctgggcc
     3661 gacggcccga ccatcaacaa caagggccag cgctgggacg ccaccaagct gggccagcag
     3721 ctcgccaccg acgtgttctt cacccgggcg ccgggcctgc gctactgaac ctgggaacat
     3781 aggggtcgcg atcgacacac cctgtcgatt ccacgcccca gaacgcccag gaggccagga
     3841 tgacccgcga catcctcgac gaaccgggcc cggcacgctt cgccgagtcc tacccgagcg
     3901 tcggtgcggc gatcggcctg gccgtgctgc accgctccac cgggctgcgc ggacgcatcg
     3961 acaagttcac cggcgacatc gtccagatcc gcgacccggc gggccgcgag caccgcttcc
     4021 gcaaccgacc gggggcgttc gccgtggccg gcgagaccgt gaccctggtg cgacccgcgc
     4081 cgcccccgac gacagcgacg ggaactctcc ggcggaccgc ggccggtgcg ctcgtgtcgg
     4141 tcgagcacac cgcgcgggtc gcccaaccgt cacggctgtg ggtggagggc agccacgacg
     4201 cccgcctgct ggaacgggtg tggggcgacg acctccgcga cctcggcatc gtggtcgaac
     4261 cgatgggcgg catcgacgac ctcgtcgccg cagtggccga gttcgacccg cgacccgacc
     4321 gccggctggc cgttctggtc gaccacctgg tcccgggctc caaggagacc cgcctggccc
     4381 agcaggtgga gggcccgaac gtcctggtgg tcgggcaccg ctacgtcgac gtgtggcagt
     4441 gcgtgcggcc cgtcgcgctg gggatcgacg cctggcccga ggttcctcgg ggtgaggagt
     4501 ggaaggccgg ggtgtgcgcc cggttgggat ggggtgaccc gcaggacggc tggcggcggg
     4561 tcctggccgc ggtcgacacc ttcgccgacc tcgaacccga cctggtcgga gccgtggagc
     4621 gcgccctcga cgtgctcgca gccgagagct gagtcgcacg gtgaccgacg gacggaccgt
     4681 cgaggacctg ctggcctccc gcctgcgccg gctcggagtc cgacgcacct acgggtcggc
     4741 cctcggcgac ctcgaccacg tgccggtcga cgatcccgac ctggcggtgc tgctggccga
     4801 cgccgacggc cgcatcggac acgtcgacgg ctcgggtcgg ctcggcgcgg ccgccctggc
     4861 cgggccgatc ctgcacctca gctcgtcgcc gggcggaacc gcgccgctgc agacgatcgg
     4921 atcggccgag gagctcgtcg aggccctcgc cgaccctccc ggcctgagcg tccccggaac
     4981 cctggccctg cacctcgacc tcgacctcca cgaaccgctc gacgccgagc cggtccccga
     5041 gtcgggtggg gaccggtccg tcgtgctgct gctcgacccg tcgctcgcgg gaatgcggat
     5101 cctggtcctg gccggcccgg gggtcgtgcg cacctactcc cacgtcgggc ttcgggacct
     5161 gagccgcgcc gccggcgccc cggtgctggc caccttcggg gcgatcggcc tggagcgatg
     5221 ggacagcccg taccacgccg gtgtcggcgg tctgcaggcc cgcgacctgc acctcggcgg
     5281 gctggacgac gccgatctcg tcatcaccac cgggctcgac ccggcggagc tgccgtccgg
     5341 gcggctggat cggctggtgg tgcaggacgt ggccccccgc cagctcggcg cgctgctgcg
     5401 cgactggccg gcgcggggtc ccgaaccgca ggaccggccg tcggtgcgcg gggcactggg
     5461 ccccgtcctc gctccgctgt gggaggccga c
//
LOCUS       DAOQOD010000193         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_5980_length_5480_cov_8.902857, whole genome shotgun sequence.
ACCESSION   DAOQOD010000193 DAOQOD010000000
VERSION     DAOQOD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5980_length_5480_cov_8.902857"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLZ72_14045"
     CDS             <1..319
                     /locus_tag="PLZ72_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37467.1"
                     /translation="VGIDHAYALTSYAVQGSTRSVSTSRVDATATRAETYVDITRGRN
                     ANHLYLTAANDPLDGEALPRIPAPPADIAVADRLHRSTGELTAWELAHPVDPALAGQA
                     IER"
     gene            398..625
                     /locus_tag="PLZ72_14050"
     CDS             398..625
                     /locus_tag="PLZ72_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37468.1"
                     /translation="MTTASADTPAEVLTVEEAAAILRISRNAAYAAARQWRATGGETG
                     LPCIEIGRTLRVPRADLDRLLGRHGPSQAVA"
     gene            645..2003
                     /locus_tag="PLZ72_14055"
     CDS             645..2003
                     /locus_tag="PLZ72_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37469.1"
                     /translation="MSFDGWLPAPGQARGAHLIEFLEALLVRCEELAEGRDWTQVAVG
                     ARDMLDAIRSAVATEPPGIAVFDFAAETVHAHLTTVGAAFDDVDPLVAAVACARAVDD
                     IWGDDLAAEWAELLASRGDKIEPGDIVMSAGSDHIADLYSLGRAFSAPRFRAADGAGR
                     TRRAGIYRPQPGVPSVLVDRSLSRVLAPALATGDQRARPWVAGVPNASSAEWEFDRPP
                     IRPRDRAGHLEATERVVADAFGLDAAVVVLPEFSGYVDVIDRLRQARPQRPTLVVAGS
                     GHESTGKTLRNVASTWIARPDGTIPPDQPIRTLKRVPYDGALGTEPLTDVASTITVHI
                     SGPWRMAIGICRDLCDPAITDALAQLGVNLLVVPACSPKTTNLARGAEAVAVDAGGFA
                     LVANGPPTFEQPNRPPAKVATAIWCTPLDRTEPRLMASTDTAGVVLVDLDGHRLIPNP
                     WP"
     gene            2300..2611
                     /locus_tag="PLZ72_14060"
     CDS             2300..2611
                     /locus_tag="PLZ72_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNA37470.1"
                     /translation="MAAETRGAVHALALVVEAAAEAARSTSVPTNEVLLAELAAAPSA
                     MLPSELADTLQLSRQTVTQALRRLADKGLVRKHAGPKADGRTVPYELTPSGRRLAAAP
                     G"
     gene            complement(2647..3342)
                     /locus_tag="PLZ72_14065"
     CDS             complement(2647..3342)
                     /locus_tag="PLZ72_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37471.1"
                     /translation="MTSDVNVIEVLGHDLLLVETSGAEVHLASLCDGDDRRAAVKATG
                     AEYVEPPEVVAGYDQAALYRERWSPKTLCGRGWVEMAAGDGGTFRRWQVISLVPTCRS
                     CLRVIDTWFAPVEAPDGMELLASVVADTVETFGCSRVVGAPVEHVEALRRAIRKHLRA
                     RGYRAETHHVNGVVHVFSEGAYAAIAPDVLAQRDREVAARIGQIISGIVREPARLQDQ
                     PDVVLWSTWVLDL"
     gene            complement(3339..5033)
                     /locus_tag="PLZ72_14070"
     CDS             complement(3339..5033)
                     /locus_tag="PLZ72_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA37472.1"
                     /translation="MRGYVVKKGKSYYAVVYDGVDPGTGKEKRKWVSAGPRRSDAEKL
                     VTDLVKRRNQGVSVATEKLTLGEYLTGRWLPIQESQIRKSTFDSYRRNIELHVLPALA
                     KMPLEKLTASDLDALYASLLVRGRKAATRTGEGLAPKTVRSIHLVVHKALADAERKGL
                     VIRNVAALADPPKVGARKQIEIKAWTAEQLDVFLEAIEAHRLAPAFHLASYTGMRRGE
                     LLGLRWKDLDLDANRLSVRQALVSVAYEMHISDVKTGSGRRTIDIEEETVEVLRQWRK
                     ARELEAGGPIGEEALVFSKPDTTWIHPDVFSQTFDRVVARIAVPEITLHDLRHTHATL
                     LLQAGVSVKVVCERLGHSNPAFTMSVYQHVLPGMQAEAAVAFAQVLRNAHMVRTAMGT
                     AVATVNGPSGKVTPLGVEGVLEALGGIEDPSTMAEIAAEAVRMMNRATLAAPSAGTVG
                     WQDVGDTYRLLGELGVLVDRLPQVCDQLARTFQRPAFRSSTRSDSETSETATSLLAVA
                     ADEMVRADEYGRALGRCIAAAHAAVSHLAPLGDDTPVMDTSTEATVVAESGEGERS"
BASE COUNT          879 a         2070 c         1708 g          823 t
ORIGIN      
        1 ggtcggcatc gaccacgcct acgcgctcac cagctacgcc gtgcagggct ccaccagatc
       61 ggtctccacc agtcgagtcg acgccacggc cacccgcgcc gagacctacg tcgacatcac
      121 tcgaggccgc aacgccaacc acctctacct caccgcagcc aacgacccac tcgacggcga
      181 agccctcccc cgcatccccg ccccacccgc cgacatcgcc gtcgccgacc ggctccaccg
      241 atccacgggc gagctcacgg cctgggaact cgcccacccc gtcgaccctg ctctggccgg
      301 tcaggcgatc gagcggtaac gccgcggggt ttgttgtcga ttttgacccc cgatcgcggc
      361 ttacccaggt acggaaacgc acaggaggcc cagtccgatg accaccgcat ctgccgacac
      421 ccccgccgag gtcctcaccg tcgaggaagc ggcggcgatt cttcgcatca gccgcaacgc
      481 cgcctacgcg gcggcccgtc agtggcgcgc caccggcggc gagaccggcc tgccctgcat
      541 cgagatcggc cgcacactcc gggtgccccg cgccgatctc gaccggctgc tgggtcgcca
      601 cggcccgtct caggccgtcg cgtgatcggt ccacaatggc ggtcatgagc ttcgatgggt
      661 ggctacccgc accaggccag gcccgcgggg ctcacttgat cgagttcctc gaggcactgc
      721 tcgtccgctg cgaggagctg gccgagggcc gcgattggac gcaggtcgcg gtcggggctc
      781 gcgacatgct ggacgcgatc cgatcagcag tcgccaccga gccgcccggc atcgcggtgt
      841 tcgacttcgc cgctgagact gttcatgccc acctcaccac ggtgggcgcc gcgttcgacg
      901 acgtggaccc gttggtcgcc gcggtggcgt gtgcccgagc ggtcgatgac atctggggcg
      961 atgatctggc cgcggagtgg gccgagctct tggcctcgcg cggcgacaag atcgagccgg
     1021 gcgacatcgt catgtccgcg ggcagcgatc acatcgctga cctgtactcg ctgggacggg
     1081 cgttctcggc acctcggttc cgcgccgctg acggtgcagg ccggacgcgc cgggcaggga
     1141 tctaccggcc gcagccggga gtcccttcgg tcctggtgga tcgttcgctc agccgagtgc
     1201 ttgcgccggc gctcgcgacc ggtgaccagc gggcacgtcc atgggtcgcc ggggtaccga
     1261 acgcgtcgag cgctgagtgg gaattcgacc ggccaccgat ccggccccgc gaccgtgccg
     1321 gccacctcga agccaccgag cgcgtggtgg ccgacgcgtt tggcctcgac gcggcggtcg
     1381 tggtcctgcc cgagttcagc gggtacgtcg atgtcatcga ccggctgcgc caagcacgcc
     1441 cgcagcgacc gaccctcgtg gttgctggca gcggccacga gtcgacaggc aagaccctcc
     1501 gcaacgtcgc atcaacctgg atcgctcgac ccgacgggac gatcccgccc gaccagccca
     1561 tccgcactct caagcgtgtc ccatacgacg gggcgctggg aaccgagccg ctgaccgatg
     1621 tcgcatccac gatcacggtg cacatctcgg ggccgtggcg catggccatc ggtatctgcc
     1681 gggacctctg cgaccctgcc atcaccgacg cgttggccca gctcggcgtg aacctgctgg
     1741 tggtcccggc ctgttcaccc aagaccacga acctggcccg cggcgccgag gccgtcgccg
     1801 tcgacgccgg aggcttcgcg ctcgtcgcca acggaccccc gaccttcgag caaccgaacc
     1861 ggccaccagc gaaggtcgcc acggcgatct ggtgcacgcc ccttgaccgg accgaaccgc
     1921 gcctcatggc cagcaccgat accgccgggg tcgtcctcgt cgacctcgac ggccaccgtc
     1981 tcatcccgaa cccctggccc tgaaccacgg atagtaaaca gatagttgct atctcggttg
     2041 gtggttcgta ccattgggtt cgagaccaca gcgcacccga ggaggtgccc cgatgccccg
     2101 cccatctccc accgatgcca aggccacgga cctgatccag ttgatcggtg ccccaacgcg
     2161 aaccgactcc ctggctcgtg gctgtggccg cacgctcatc gctgggccat cacgaccggc
     2221 actgctcgag ctgctcggag agctcggcgc gtgggagcac cgcctggcat cggaggccgc
     2281 gacgacgacc gacccggtca tggcagccga gacccgcggc gcggtccacg cccttgcgct
     2341 ggtcgttgaa gccgcggccg aagccgcacg cagcacctcg gtgcccacca acgaggttct
     2401 gctcgccgag ctcgcggctg cacccagcgc gatgctgccc agcgagctgg ccgacacgct
     2461 ccagctgtca cgccagaccg tcacccaggc gctgcgtcgc ctggccgaca agggcctggt
     2521 ccgcaagcac gccggcccca aggccgacgg ccgcacggtc ccctacgagc tgaccccatc
     2581 gggtcgacgc ctggccgcgg cgcctggctg accgcaccac gccggtcggg ccaggcgacc
     2641 gctggctcac aggtcgagga cccaggtgct ccacaagacg acgtcgggct ggtcttggag
     2701 ccgggccggt tcgcgaacga tccccgagat gatctggccg atccgggcag cgacttcgcg
     2761 gtcgcgctgc gcgagcacgt ccggggcgat cgccgcgtac gcgccctcgg agaacacgtg
     2821 caccacaccg ttgacgtggt gggtttcggc ccggtagccc cgggcgcgga ggtgcttgcg
     2881 gatcgcccgg cggagcgctt cgacgtgttc gaccggggcg cccaccaccc gtgagcaccc
     2941 gaacgtctcc accgtgtcgg ccacgaccga cgccagcaac tccatcccgt cgggagcttc
     3001 tacgggcgcg aaccacgtgt cgatcacccg cagacacgac cgacacgtcg gcaccagcga
     3061 gatcacctgc caacgacgga acgtgccgcc gtcacccgcg gccatctcga cccacccacg
     3121 gccgcacaag gtcttgggcg accagcgctc gcgatacagc gcagcctggt catagccggc
     3181 aacgacctcc ggtggctcga cgtactcggc gccggtcgct ttcaccgccg cccgccgatc
     3241 atcaccgtcg cacagcgacg ccaggtgcac ttccgctccg ctggtctcga ccaacagaag
     3301 gtcgtgtccc agcacctcga tcacgttcac atcgctggtc acgaccgctc cccctctccg
     3361 ctctcggcca cgactgtggc ctccgtcgag gtgtccatga cgggggtgtc gtcgccgagc
     3421 ggggccaggt gcgacacggc ggcatgagcc gcggcgatgc agcggcccag tgcccgcccg
     3481 tactcgtcgg ctctgaccat ctcgtccgcc gcgacagcca gcaacgacgt cgctgtctcg
     3541 gaggtctccg aatcgctgcg ggtcgatgac cgaaacgcgg gccgctggaa cgtgcgggcc
     3601 agctgatcac acacctgggg cagtcggtcg acgagcacac ccagctcgcc caacagccgg
     3661 taggtgtcgc ccacgtcctg ccacccaacc gtgcccgccg atggcgcggc aagggtggcg
     3721 cggttcatca tgcgcaccgc ttcggccgcg atctccgcca tcgtcgaggg gtcctcaatg
     3781 ccacccaacg cctcgagcac accttcgaca ccgagtgggg tcaccttccc tgacggcccg
     3841 tttaccgtcg ccacggcggt ccccatcgcg gtcctgacca tgtgggcgtt gcgcagcacc
     3901 tgcgcgaacg cgacggccgc ttcggcctgc atgcccggca acacgtgctg gtacaccgac
     3961 atcgtgaacg ccgggttcga atgcccgagc cgttcgcaca ccaccttcac cgacaccccc
     4021 gcctgcaaca gcagcgtcgc atgggtatgg cgcaggtcgt gcaaggtgat ctcggggacc
     4081 gcgatcctcg cgacgactcg gtcgaaggtc tggctgaaca cgtcagggtg gatccacgtg
     4141 gtgtcgggct tggagaacac caacgcctcc tcgccgatcg gtccaccggc ctcgagctca
     4201 cgggccttgc gccactggcg gaggacctcg acggtctcct cttcgatgtc gatcgtgcgc
     4261 cgccccgacc cggtcttcac atcggagatg tgcatctcat aggccaccga caccaacgcc
     4321 tggcgcaccg acagacggtt cgcgtcgagg tcgaggtcct tccaacgcaa ccccaacaac
     4381 tcaccccggc gcatccccgt gtacgacgcc agatggaacg ccggggcgag acggtgcgcc
     4441 tcgatcgctt cgaggaacac gtcgagctgc tccgcggtcc acgccttgat ctcgatctgc
     4501 ttgcgggcgc cgaccttggg cggatcggcc agcgcggcga cgttgcggat caccaggccc
     4561 ttgcgctccg cgtccgcgag cgccttgtgc accaccaggt ggatcgaccg caccgtcttc
     4621 ggtgccagac cctcaccggt gcgagtcgcc gccttgcggc cccgcaccag aagcgacgcg
     4681 tacaacgcgt cgaggtcgct cgcggtcagc ttctccaacg gcatcttggc cagcgccggc
     4741 aacacgtgca gctcgatgtt gcgccgatac gaatcgaacg tgctcttgcg gatctggctc
     4801 tcctggatcg gcaaccaccg ccccgtcagg tactcaccca gcgtcagctt ctcggtcgcg
     4861 accgacactc cctggttgcg gcgcttcacc agatcggtca ccagcttctc cgcgtcgctg
     4921 cgacgcggac ccgccgagac ccacttgcgc ttctccttgc ccgtaccggg gtcgacaccg
     4981 tcgtagacga ccgcgtagta gctcttgccc ttcttgacga catagccgcg catggctgtt
     5041 ccttcgaggt agtccagaag gaacgccgag cagccttgcg tccgcacccg gggcgttcga
     5101 cgggcaacgc gggactgtgc cgatcagcct acgcccactc gggggaacgg tctaccggcc
     5161 ccttcgaccc gcgcgttcta ccgagaaccc gaagatcacc ccaaccgccc tccccaagcc
     5221 ccgcgagcgg tccgaaccca ggccacgcaa ggacggccgt catcccggcc cggaaggtcc
     5281 cgtcacggac cttcgtgggc tgtaccagcc gatccggtag aaaacccggt agaagatcgg
     5341 gggaacaggc agcagaaagg ccctccccga ttcctcggaa agggcctttg acctgctgtt
     5401 tcagatggtg gcgggggcag gatttgaacc tgcgaccttc gggttatgag cccgacgagc
     5461 taccagactg ctccaccccg
//
LOCUS       DAOQOD010000194         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6037_length_5448_cov_13.472093, whole genome shotgun sequence.
ACCESSION   DAOQOD010000194 DAOQOD010000000
VERSION     DAOQOD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_5448_cov_13.472093"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLZ72_14075"
     CDS             <1..1022
                     /locus_tag="PLZ72_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37473.1"
                     /translation="GVTVTAGTAGDGGAGQSGGAGGSGGSGGPGGNKSCCEGGGAGGP
                     SIGLLHSGSGSVATSGSPIGAAPTASAGGNGGAAGAAGVGGAPGNCANIGGCGPSGGA
                     SSGSGNAGTAGRSGAAGLRYGIWDNGATTDPSHSGTTTSSTTTSSTAPAAQPVHTAVA
                     MSCYTTAAGQTSYDANDTGATVLAPTTVAAGADFDVELTPDPMAVPTSGGGYPIGYVA
                     NVQYRFNVPAGATFVGATYSGGSNLGSGTPSVAVSGGKVVLTVPGTLAAGVSANFPKV
                     TVTFQATGAAGTSLGFAYAGTSYGDPALTFQTRVNNIPILGSVTSTSNCYAATNPILA
                     TTVIS"
     gene            complement(1106..2509)
                     /locus_tag="PLZ72_14080"
     CDS             complement(1106..2509)
                     /locus_tag="PLZ72_14080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA37474.1"
                     /translation="MTFPTATPSSQGIDPVALADFVDAVDTDPRIEAHGLIVQRRGRR
                     VLEAYWSPHRAGEARLVYSLSKSFTGAALGLAVGDGLVSLDDRVADHLGDLMVDPGPL
                     TERTLIRHIASMSSGHRDETLLDAFMAEPDDPLRGYFTLEPDHEPGTWFAYNQPPVLA
                     LSALLHRVTGRRLVDQLRARVLEPIGIGNLRWYQYRPGVDLGFSGVFTDLDAIARFGQ
                     LHLDDGVWDGRRLLPEGWVGLASSIQVTNEQRIEPDWSRGYGFQLWMCRHGFRGDGAF
                     GQYMVVLPEQDAVVAFFSHTEDMQSFMDLIWDRLLPAFDPPRTPDPSADAAVAERTSA
                     LQHPTASARSGWAGSPSVAVRGSFTRADGVASHSSITGVEIDGAHLLLHEGDLVTRVP
                     LSSSWVDVAGVPMSASAAADGDGRLTVDLVLRASPHRLELTTDPTTSTFTATWPLFPL
                     FGMGLDTALARIGPPPD"
     gene            complement(2584..3324)
                     /locus_tag="PLZ72_14085"
     CDS             complement(2584..3324)
                     /locus_tag="PLZ72_14085"
                     /inference="COORDINATES: protein motif:HMM:NF005879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HNA37475.1"
                     /translation="MTELVHHDVADGVGTITLDSPHNRNALSRQLVTELFAALEAARS
                     DDSAVVVLRSADRVFCSGADLSEATEGAMEQGTRALVDLQRLIVTHPKPVVVRLAGPV
                     RAGGLGLVASADIAICSDDVTFAFTEARLGVAPAVISLTVLARMTSRAAFDTFLTGRT
                     FDAAEAAAMGLVTRSVPADELDDEIARVCDDLRQAHPQGLAETKALLAREVVARIDAG
                     ADDMAALSARLFASDGAREAITAFLQRR"
     gene            complement(3365..4522)
                     /locus_tag="PLZ72_14090"
     CDS             complement(3365..4522)
                     /locus_tag="PLZ72_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37476.1"
                     /translation="MFAEPEERVALRDAVAKLAGTYGREYVERQAAAGDKMTDMWLEM
                     GRSGFLGVNLPEEYGGGGGGMADLAAVLEECATAGAPLLMMVVSPAICGSIIVRCGTE
                     EQKRRWVPGLADGTLLMAFGITEADAGSNSHQITTTARRDGDEWTLNGQKTFISGVDE
                     AQSVLIVARTEDEKTGKLRPALFVVPTDTPGFSRSPIEMGWAAPEKQFTLFLDDVRLP
                     HDALIGEQDGDLWQLFAGLNPERIMGAAMSCGIARYALGRAVEYAKERKVWKDQPIGA
                     HQGIAHPLARIKIELEQARLLWQKAAALYDAGDDFGAGEYANMAKYAGGEIACNATDV
                     AVHTHGGNGLTVEYGLANMLVAARLGRIAPVSREMILNFVAMHSLGLPKSY"
     gene            complement(4528..>5448)
                     /locus_tag="PLZ72_14095"
     CDS             complement(4528..>5448)
                     /locus_tag="PLZ72_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37477.1"
                     /translation="GVGAGDEISTFYDAMIAKVVAWAPTRDQALRRLAAALADAEIHG
                     LRTNRNLLVNLLRDPVVRDGRMDTTWLDGADLSTLGRCVTSPDAVGLSAFAATIAVAE
                     QARMQRPVQQRITSGYRNVVAEPLLAAFEDTDGEPVDVRWHGGATPCPADRDDVRVTG
                     VELPVGSGQGDPVVVRLDVAGVTHTFRVHVDGRHVDVESALGHVALTRRPRLVDPAGL
                     VAEGSLLAPMPGSVIDVRVGVGDHVSEGDTLVVMEAMKMQHAIAAPAAGVVVELGAEV
                     GSQVDAGTVLAVVDADAAESLEPAHEGETP"
BASE COUNT          783 a         1887 c         2006 g          772 t
ORIGIN      
        1 ccggggtgac ggtcaccgcg gggaccgccg gagacggtgg ggccgggcag tccggtggag
       61 ccggcggttc gggcggttcg ggcggtcccg gcggcaacaa gtcctgctgc gaaggcggcg
      121 gcgccggcgg tccgtccatc ggcctgctgc acagcgggtc gggctctgtg gcgaccagcg
      181 gttcgccgat cggagcggct ccgaccgcgt ccgcgggcgg caacggcggt gctgccggag
      241 cggcaggagt cggcggagcc ccgggcaact gcgccaacat cggcggctgc ggcccgagcg
      301 gtggagcttc ctcggggtcc ggcaacgccg ggaccgctgg tcgctccggt gctgccgggc
      361 ttcgctacgg gatctgggac aacggggcga cgacggaccc gtcgcactcg ggcaccacga
      421 cgtcatcgac gacgaccagc tcgaccgcac cggcggccca gcccgtgcac accgcggtgg
      481 cgatgagctg ctacacgacc gccgcgggcc agacgagcta cgacgccaac gacaccggcg
      541 cgaccgtgct cgcgccgacg acggtcgcgg caggcgccga cttcgacgtc gagctgaccc
      601 cggacccgat ggcggtgccc acctcggggg gcggctaccc catcggctac gtggccaacg
      661 tccagtaccg gttcaacgtg ccggcggggg cgacgttcgt cggagccacg tacagcggcg
      721 gctccaacct gggttcgggg acaccgagcg tggccgtgag cggcggcaag gtggtcctga
      781 ccgtccccgg cacgctcgcc gcgggcgtgt cggcgaactt ccccaaggtg accgtcacgt
      841 tccaggccac cggcgccgcg ggcacgtcgc tcgggttcgc ctacgccggg acctcctacg
      901 gcgatccggc cctcacgttc cagacccggg tgaacaacat cccgatcctg ggatcggtca
      961 cctcgacgtc gaactgctac gccgcgacca acccgatcct ggccacgacc gtcatcagct
     1021 gacgcagacg gcctgttctg tagtgcgaga tccaggcgaa cgcctggatc tcgcactaca
     1081 gaagcggaat cgggagggtc ggcggtcagt ccggtggggg gccgatccgg gccagcgccg
     1141 tgtccaggcc catcccgaac aacgggaaca gcggccaggt tgccgtgaac gtcgatgttg
     1201 tcgggtcggt ggtcagctcc aaccggtgcg gtgacgcccg caacaccaga tccacggtga
     1261 gccggccgtc tccgtccgcg gctgcgctgg ccgacatcgg gactccggcg acgtcgaccc
     1321 agctcgagct cagcgggacg cgggtcacca ggtcgccctc gtgcaacagc aggtgggcgc
     1381 cgtcgatctc gacccctgtg atgctcgagt ggctcgccac cccgtcagcc cgggtgaacg
     1441 agccccggac cgctaccgac ggtgacccag cccagcccga ccgggccgag gcggtggggt
     1501 gctgcaacgc cgaggtgcgc tcggcgaccg ccgcgtcggc actgggatcc ggggtccggg
     1561 gcgggtcgaa ggcagggagc agccgatccc agatcaggtc catgaacgac tgcatgtcct
     1621 cggtgtggga gaagaacgcc acaaccgcgt cctgctccgg cagcaccacc atgtactggc
     1681 cgaaggcgcc gtcgccgcgg aacccgtgcc gacacatcca gagctggaac ccgtagcccc
     1741 ggctccagtc cggttcgatc cgttgctcgt tcgtcacctg gatgctggac gccaagccca
     1801 cccacccctc ggggagcagc cgcctgccgt cccatacgcc gtcgtcgagg tggagctggc
     1861 cgaaccgggc gatggcgtcg agatcggtga acacgcccga gaagcccagg tcgaccccgg
     1921 gccggtactg gtaccagcgc agattgccta tcccgatcgg ttcgagcacc cgagcccgga
     1981 gctggtcgac gagccgccgg ccggtgaccc ggtggaggag cgccgacagg gcgagcacgg
     2041 gtggctggtt gtaggcgaac caggtcccgg gctcgtgatc gggctcgagc gtgaagtagc
     2101 cgcgcagcgg atcgtcgggt tcggccatga aggcgtcgag cagggtctcg tcgcggtgac
     2161 cgctggacat ggatgcgatg tgacggatca gtgtgcgctc ggtcagcggt ccggggtcga
     2221 ccatcaggtc gccgaggtgg tcagcgacgc ggtcgtcgag cgacaccagc ccgtccccga
     2281 cggccaggcc cagagcagcc ccggtgaagg acttgctcag cgagtacaca agccgggcct
     2341 ctccggcgcg gtggggactc cagtaggcct ccagcacccg gcggccgcgg cgctggacga
     2401 tcagcccgtg cgcctcgatg cggggatcgg tgtcgaccgc gtcgacgaag tcggccagtg
     2461 cgacgggatc gatcccctgc gatgacggtg tggcagtcgg gaacgtcatc ggctcatctt
     2521 gctctgtcac gcacagaagc ggtgatcacc gcgtccctgc gggacaaagc ggggcggtgg
     2581 gggtcagcgg cgctgcagga aggcggtgat cgcttcgcgg gcgccgtcgc tggcgaacag
     2641 ccgggcgctg agagcggcca tgtcgtcggc tccggcatcg atgcgcgcca ccacctcgcg
     2701 ggccagcagc gccttggtct cggcgagccc ctgagggtgg gcctgtcgga ggtcgtcgca
     2761 gacccgggcg atctcgtcgt cgagctcgtc ggcgggcacg ctgcgggtga ccaggcccat
     2821 cgccgcggcc tcggccgcgt cgaaggtgcg accggtcagg aacgtgtcga acgcggcgcg
     2881 gctggtcatg cgggccaaca cggtcaggct gatcaccgcc ggggcgacac ccaggcgcgc
     2941 ctcggtgaac gcgaacgtga cgtcgtcgga gcagatcgcg atgtcggcgc tggcgaccag
     3001 gccgaggccc ccggcccgca ccggtccggc caggcgcacc accaccggct tggggtgcgt
     3061 gacgatcagg cgctggagat cgaccagcgc ccgggtgccc tgctccatgg cgccctcggt
     3121 cgcctcgctc aggtccgctc ccgagcagaa gacccgatcc gcgctccgca gcacgaccac
     3181 ggccgagtcg tcactccggg cggcctcgag cgcggcgaac agctcggtga cgagctgacg
     3241 gctcagcgcg ttgcggttgt gcgggctgtc gagggtgatg gtgccgacgc cgtcggcgac
     3301 atcgtggtgc acgagctcgg tcatcgggat ctccgcggtt cgaatcgggt cagtacgtcg
     3361 tgggtcagta cgacttgggc aggccgagcg agtgcatggc cacgaagttc aggatcatct
     3421 cgcgcgacac cggcgcgatg cggccgagcc gggcggcgac gagcatgttg gccaggccgt
     3481 actcgacggt gagcccgttg ccgccgtggg tgtgcacggc cacgtcggtg gcgttgcagg
     3541 cgatctcgcc gcccgcgtac ttggccatgt tggcgtactc gccggcaccg aagtcgtcgc
     3601 ccgcgtcgta gagcgcggcc gccttctgcc acagcagccg ggcctgctcc agctcgatct
     3661 tgatgcgggc gagcgggtgg gcgatgccct ggtgcgcgcc gatcggctgg tccttccaga
     3721 ccttgcgctc cttggcgtac tcgacggcgc ggccgagcgc gtagcgggcg atgccgcacg
     3781 acatggcggc gcccatgatg cgctcggggt tcaacccggc gaacagctgc cacaggtcgc
     3841 cgtcctgctc gccgatcagc gcgtcgtgag gaaggcgcac gtcgtcgagg aacagcgtga
     3901 actgcttctc gggggcggcc cagcccatct cgatggggga gcggctgaac ccgggggtgt
     3961 cggtgggcac cacgaacagc gccgggcgca gcttgccggt cttctcgtcc tcggtgcgcg
     4021 caacgatcag caccgactgc gcctcgtcga ctccggagat gaaggtcttc tggccgttga
     4081 gggtccactc gtcgccgtcg cggcgggcgg tcgtggtgat ctggtgcgag ttcgatccgg
     4141 cgtcggcctc ggtgatgccg aacgccatca gcagcgtgcc gtcggccagg cccggtaccc
     4201 agcggcgctt ctgctcctcg gtgccgcagc gcacgatgat cgacccacag atcgccggag
     4261 acacgaccat catcagcagc ggggcgcccg cggtggcgca ctcctcgagc acggcggcca
     4321 gatcggccat gccgccgccg cccccgccgt actcctcggg caggttcaca ccgaggaagc
     4381 cgctgcggcc catctcgagc cacatgtcgg tcatcttgtc gcccgctgcg gcctggcgtt
     4441 cgacgtactc gcggccgtac gtgccggcga gcttggcgac cgcgtcgcgc agggcgacgc
     4501 gctcctcggg ttcggcgaac atggtggtca cggggtctct ccttcgtggg ccggctcgag
     4561 cgactcggcg gcgtcggcat cgacgacggc gagcacggtg ccggcgtcga cctggctgcc
     4621 gacctcggca cccaactcga cgacgacgcc ggccgcgggc gcggcgatgg cgtgctgcat
     4681 cttcatggct tccatgacca cgagcgtgtc gccctcgctc acgtggtcgc cgacaccgac
     4741 gcgcacgtcg atgaccgagc cgggcatggg ggcgaggagc gacccttcgg cgaccagccc
     4801 ggcggggtcg accaaccgtg gtcggcgggt gagcgcgacg tgtccgagcg cggactcgac
     4861 gtcgacgtgg cgaccgtcga cgtggacgcg gaaggtgtgg gtgacaccgg cgacgtcgag
     4921 gcggacgacg acgggatcgc cctgtccgga cccgacgggc agttcgacgc cggtcacccg
     4981 gacgtcgtcg cggtcggcgg ggcatggcgt cgctccgccg tgccagcgga cgtccaccgg
     5041 ctcgccgtcg gtgtcctcga acgctgcgag caggggctcg gcgaccacgt tgcggtagcc
     5101 ggacgtgatg cgctgctgca ccggacgttg catgcgggcc tgctcggcca ccgcgatcgt
     5161 cgccgcgaac gcgctgaggc ccaccgcgtc cggggacgtg acgcagcggc cgagtgtgga
     5221 caggtcagct ccgtccagcc aggtcgtgtc catgcggccg tcccgcacca ccgggtcgcg
     5281 cagcaggttc accagcaggt tgcggttggt gcgcaggccg tggatctcgg cgtcggccag
     5341 agcggcggcg aggcggcgca acgcctggtc gcgggtcggt gcccacgcga cgaccttggc
     5401 gatcatcgcg tcgtagaagg tgctgatctc gtcgccggca ccgacgcc
//
LOCUS       DAOQOD010000195         5387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6157_length_5387_cov_13.121530, whole genome shotgun sequence.
ACCESSION   DAOQOD010000195 DAOQOD010000000
VERSION     DAOQOD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6157_length_5387_cov_13.121530"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..989
                     /locus_tag="PLZ72_14100"
     CDS             108..989
                     /locus_tag="PLZ72_14100"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HNA37478.1"
                     /translation="MDEQQLNKITNDPGFIAALDQSGGSTPKALKLYGIDESEYSGED
                     EMFDLIHQMRTRIITDPAFNGDRIVGAILFEQTMDRQIDGKGTAEYLWQDKGVVPFLK
                     VDKGLADEVDGSQVMKPIPGLDDLLERAKTHGVFGTKMRSVIKVANEAGVTANVAQQF
                     EIGRQILAHGLVPIIEPEIDINSPSKAEAEKLLKERILAELDGVDGQIMLKLTLPEQD
                     GFYTELVEHPKVLRVVALSGGYSREEANARLARNPGVIASFSRAFTEGLSAQQSDEEY
                     TAALDASTQSIFEASNT"
     gene            1053..3230
                     /locus_tag="PLZ72_14105"
     CDS             1053..3230
                     /locus_tag="PLZ72_14105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNA37479.1"
                     /translation="MTVRPVSEHTSEAGRRRVGDPFTNLPGLDGLRGLAVVAVVAFHA
                     GLGRMVGGFLGVSTFFTLSGFLVTGLLVRRSRHSPAIALRPFWERRARRLLPASLATL
                     AGIVLLFGPFVATAEQRRAMPADVLAALFEVANWHDIASGSSYGALFAAPSPVLHFWS
                     LSIEAQFYVALPVLVLAAWRFGRRRRVTLAAILGGLALLSALEPVVFSMSPDRVYYGT
                     DTRAVELLIGGLLGLAWTHEPIRRRLVIGRGVRVTVPALGALALGLQLWWWWSLPLST
                     PWLSRGGFTGYAVLSCLVILAAAVPVGPVNAVLRSAPLRWLGVRSFGIYLLHWPVMLA
                     VRQLHPGVAPATSATIAVVISVLGAAVSYRLLEQPVRTGRWPAPGRVLRTSTATVAVM
                     AVATLTLPSGSRHESAGGLVADPATYDRLIEAHRSAASPHSTNPARPDADPVASSTVA
                     VAPVPTMAPFGDSTVLGVGMALARWAMDTGRLTGTRGDFRVGCGIPRFDAIRTTGVKP
                     DDPECRDWPGRWAERIRTDSPDIALIMSAVWSVPDARLPGSDRWSAIGDPHTDEFIRD
                     EYLAAVDVLSSRGALVVLVTWPRFGDWVAGGRSGVLGDQIDGERMSRMNRILTEVAAL
                     RPESVRVVDLAGWLGDRSQDRALRPDGVHFSEATFRPIVDEWMGPEIERIWTEWWTRH
                     RTSAAADPAAPGPATPDPATPQPAVQIPGLAASTPEGPTTGRA"
     gene            complement(3234..3845)
                     /locus_tag="PLZ72_14110"
     CDS             complement(3234..3845)
                     /locus_tag="PLZ72_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNA37480.1"
                     /translation="MHAPQLVDLVGSKRAVRLEGRLSDAATAYAAERYPEARAVLAPV
                     VAEVPDLAEARELYGLTLYRLGRWREAARELDAFVELTGGSTEQHPVLADCRRALGQY
                     SRVEELWEELRTSSPSGPLVNEGRIVAAGALADQGRLADAIALLGKGFRIPKRPMDHH
                     LRRAYALADLYERSGDVPQARLLFTQVARAEPDFLDAAERAAG"
     gene            complement(4713..4829)
                     /gene="rrf"
                     /locus_tag="PLZ72_14115"
     rRNA            complement(4713..4829)
                     /gene="rrf"
                     /locus_tag="PLZ72_14115"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4920..>5387)
                     /locus_tag="PLZ72_14120"
     rRNA            complement(4920..>5387)
                     /locus_tag="PLZ72_14120"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          778 a         1950 c         1836 g          823 t
ORIGIN      
        1 ggtttggtcc gccccggagc cgctgtggga tcctgtggca ggccgcgctg tggccgagtc
       61 gaccagtgga ccgtgtcgac cacagacccc tgcaggagga ctcttccgtg gacgagcagc
      121 agctcaacaa gatcaccaac gatccgggct tcatcgccgc cctcgaccag agcggtggat
      181 ccacgcccaa ggcgctcaag ctctacggca tcgacgagag cgagtactcc ggcgaggacg
      241 agatgttcga cctcatccac cagatgcgga cccgcatcat caccgacccg gcgttcaacg
      301 gcgaccgcat cgtcggggcg atcctcttcg agcagacgat ggaccgccag atcgacggca
      361 agggcaccgc ggagtacctg tggcaggaca agggcgtggt gccgttcctg aaggtcgaca
      421 agggcctggc cgacgaggtc gacggcagcc aggtcatgaa gccgatcccc ggcctcgacg
      481 acctgctcga gcgggccaag acccacggcg tgttcggcac caagatgcgc tcggtcatca
      541 aggtcgccaa cgaggccggt gtgaccgcca acgtggccca gcagttcgag atcggccgcc
      601 agatcctggc ccacggcctg gtcccgatca tcgaaccgga gatcgacatc aacagcccct
      661 ccaaggccga ggccgagaag ctcctcaagg agcgcatcct ggccgagctc gacggcgtcg
      721 acggccagat catgctgaag ctcaccctcc ccgagcagga cggcttctac accgagctgg
      781 tcgagcaccc caaggtgctg cgggtcgtcg ccctctccgg cggctacagc cgtgaggagg
      841 ccaacgcccg cctggcccgc aaccccggcg tgatcgccag cttctcccgg gccttcaccg
      901 agggcctgtc ggcccagcag tccgacgagg agtacaccgc ggcgctcgac gcgtccaccc
      961 agagcatctt cgaggcctcc aacacctgac ccggccggtc gatccactcg gccggggtgc
     1021 cccgacggcc ctcgggctga caacatccga cgatgaccgt gcggcccgtc tccgagcaca
     1081 cctcggaggc gggccgccgt cgcgtcggtg acccgttcac gaatctgccg ggtctcgacg
     1141 gcctgcgagg gttggcggtg gtcgcggtcg tcgccttcca cgcgggcctc ggtcgcatgg
     1201 tcggcggctt cctgggcgtc tcgacgttct tcacgctgtc gggcttcctc gtcaccgggc
     1261 tgctggtccg ccggtcccgg cactcccctg ccatcgccct ccgtccgttc tgggaacgac
     1321 gggcacgacg gctgctgccc gcatccctgg ccacgctggc tgggatcgtg ctcctgttcg
     1381 gcccgttcgt cgccacggcc gagcagcgca gggcgatgcc tgccgacgtg ctcgccgccc
     1441 tgttcgaggt cgccaactgg cacgacatcg ccagcggcag ctcgtacggg gcgttgttcg
     1501 ccgcgccgtc gccggtgctg cacttctgga gcctgtcgat cgaggcccag ttctacgtgg
     1561 cgctgcccgt gctggtgctc gccgcgtgga ggttcggacg gcgacggcgg gtgacgctgg
     1621 ccgcgatcct cggcggactc gccctgctgt cggcgctgga gcccgtcgtg ttctcgatga
     1681 gccccgaccg cgtctactac gggaccgaca cccgggccgt cgagctcctc atcggaggcc
     1741 tgctcggcct ggcgtggacc cacgaaccga tccgacggcg gctcgtcatc ggccgcggcg
     1801 tgcgggtcac ggtgccggcg ctgggagcgc tcgcactggg gctgcagctg tggtggtggt
     1861 ggtcgttgcc gctctccacc ccgtggctct cgcggggcgg cttcaccggc tacgcggtgc
     1921 tgtcctgcct ggtgatcctg gccgccgcgg tgccggtcgg tccggtgaac gccgtcctgc
     1981 ggtcagcacc gttgcgctgg ctcggcgtgc gctcgttcgg catctacctc ctgcactggc
     2041 ccgtcatgct cgctgtgcgc cagctgcacc ccggggtggc gccggcgaca tcagcgacga
     2101 tcgcggtggt gatctcggtg ctcggtgccg ccgtgtcgta ccgcctcctc gagcagccgg
     2161 tgcgaacggg ccgatggccc gcacccgggc gcgtccttcg gacctccacc gcgacagtgg
     2221 ccgtgatggc ggtcgccacg ctcaccctcc cgtcggggag ccgtcacgag tccgccggtg
     2281 gcctggtcgc cgatccggcc acctacgatc gcctgatcga ggcccacagg tccgcggcgt
     2341 cgccccactc gacgaacccg gcccgacccg acgccgatcc cgtcgcgtcg agcaccgtcg
     2401 ccgtggcacc ggtccccacg atggcaccct tcggggactc cactgtcctc ggggtcggga
     2461 tggccctggc ccgctgggcg atggacaccg ggcggttgac cgggacccgg ggcgacttcc
     2521 gggtggggtg cgggatcccc cgcttcgacg cgatccgcac caccggcgtc aagcccgacg
     2581 atcccgagtg ccgggactgg cccgggcgat gggcggaacg gatccgcacc gactcccccg
     2641 acatcgcgct gatcatgtcg gcggtgtggt cggtcccgga cgcccggctc cccggatctg
     2701 accgctggag cgcgatcggc gatccccaca ccgacgaatt catccgggac gagtacctcg
     2761 ccgcggtcga cgtgctgtcc tcccggggcg cactggtggt gctggtcacc tggccccggt
     2821 tcggcgactg ggtcgccggc ggacgatcag gggtgcttgg agaccagatc gacggggaac
     2881 gcatgtcgcg gatgaaccgc atcctcaccg aggtggcggc cctacgaccg gagtcggtcc
     2941 gcgtggtcga cctggccgga tggctgggcg accggtccca ggatcgggct ctgcgacccg
     3001 atggcgtcca cttcagcgag gcgacgttcc gcccgatcgt cgacgagtgg atggggcccg
     3061 agatcgagcg gatctggacg gagtggtgga cccggcatcg gacgtccgcg gcggccgatc
     3121 ccgcggctcc tggtcccgcg acgccagatc ccgcgacgcc acaaccggcc gtgcagatcc
     3181 cgggacttgc cgcgtcgacg ccggagggcc cgaccaccgg ccgggcctga acgtcaaccc
     3241 gcggcacgct ccgcggcgtc gaggaagtcg ggctcggccc gggccacctg ggtgaacagc
     3301 agccgggcct ggggcacgtc gccggaccgc tcgtagaggt cggccagggc gtaggcccga
     3361 cggaggtggt gatccatcgg ccgtttgggg atccggaacc ccttgcccag cagagcgatg
     3421 gcgtcggcca gtcgaccctg gtcggcgagg gcgcccgcgg cgacgatccg gccctcgttg
     3481 accagcggac ccgacggcga cgacgtccgc agctcctccc acagctcctc gacacggctg
     3541 tactggccga gcgcccgacg gcagtcggcg agcaccggat gctgctcggt cgatcctccg
     3601 gtcagctcga cgaaggcatc gagctcacgg gcggcctcac gccagcgacc cagccggtac
     3661 agggtgagcc cgtagagctc acgggcctcg gccaggtccg gcacctcggc caccaccggg
     3721 gcgagaacgg cccgcgcctc ggggtagcgc tctgccgcgt acgccgtcgc agcatccgac
     3781 agccgaccct cgaggcggac cgcccgcttg gagcccacca ggtcgaccag ctgcggagcg
     3841 tgcaccttga ccggtcgccc agatcgccgg gacgagctcc gccggggtgg ccgcgacgac
     3901 acagggccct cgtcgatcca cacctctgcc tcgcgggccg gagcggccgg gggtcgatcg
     3961 gacttgtcgg tccgccgcgg cggcggaggg atcggtcggg aatcgcgctg acggctccgg
     4021 gccgcgccct ggcgtgtgcc ctggcgcccg tcccgatccc ggtccctgcg atcccggtcg
     4081 ttgcgatccc gtcgctcacg gtccggacga ggacctgccg gtcgtgagcc ctgcggacgc
     4141 ggaccgctgc gtgctccgcc tggtcgcgac ccgccgttgc gggccgggcc gctccgagct
     4201 ccgccgctgc gggatccacc cgacgaacgt gaaccaccat ctcgcgactc tccgccgcgg
     4261 gatgcgccac gcccagcccc accgtctcgt gacccgcccg ggcgcccgga ggtgccccgc
     4321 gtaccgccgg agcgcggtcc tcctgcgccc gaaccggatg accgggtgcc ggatgaccgg
     4381 gtgccggatg accgggtgcc ggaagagcgg ggccccgaag accgagcacc gcctgagcgt
     4441 gacccccccg aggaccctcc ggatggcgat cgggagcctg aacgcgagcc ctgggagcct
     4501 gtactgcgcc gcggcggacc agcatcatcg tcggagtcgg gtcgccgccg gggcggtcgg
     4561 tcgttggcgg gggtgggcat caggacctct tcaacagcgt cgacggctcg aacgcgagtg
     4621 gccacgctac cggacgcagc gcggcggccg gcatggccga cgggacggga aaacggtgaa
     4681 ggggaggcgc tctcgcgcct ccccttcgag aaatccggcg gcgtcctact ctcccaggga
     4741 cgaacgtccc aagtaccatc ggcgctggag ggcttaactt ccgtgttcgg gatgggaacg
     4801 ggtgtgaccc ctccgctgtg gccaccgaaa tctgttgtca actgacgagc gggtggcccc
     4861 gactccgggg ccggcgtcca ctcgaggact tcatagcgag cacgaacgtc ttgtaagacg
     4921 aacccaagcc ctcggccgat tagtaccggt cagctgaaca cattgctgtg cttacacttc
     4981 cggcctatca acgtggtgat ctgccacggg ccttaccagg ttgaccctgt gggtgaactc
     5041 atcttggaag aggcttcccg cttagatgct ttcagcggtt atcccgtccg taggtcgcca
     5101 accagccgtg cccttggcag gacaactggc acacgagagc tacgtccatc ccggtcctct
     5161 cgtactaggg atagctttcc tcaattcacc tccggctgca gaggataggg accgaactgt
     5221 ctcacgacgt tctaaaccca gctcgcgtac cgctttaatg ggcgaacagc ccaacccttg
     5281 ggacctgctt cagccccagg atgcgatgag ccgacatcga ggtgccaaac cttcccgtcg
     5341 atatggactc ttggggaaga tcagcctgtt atccccgggg taccttt
//
LOCUS       DAOQOD010000196         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6205_length_5363_cov_10.055765, whole genome shotgun sequence.
ACCESSION   DAOQOD010000196 DAOQOD010000000
VERSION     DAOQOD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_5363_cov_10.055765"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..703)
                     /locus_tag="PLZ72_14125"
     CDS             complement(122..703)
                     /locus_tag="PLZ72_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37481.1"
                     /translation="MPVERRSGSQQTRSAIIAVVALVIAVLGAWGMIRLASGGQGPVK
                     VQLGDDTFDAGYATRMADQIDKDGPLLFSDVSGRGQRQPIYVVHIGSDDKDGWFALSA
                     IAPGAAEGCFVTWNPESELFEERRAADPAEGRDALGERCRDVTWSADGTDGSDGSELE
                     AFPWEIDKDERLIIDLRPDQSRTTTTTIATSGN"
     gene            804..>1582
                     /locus_tag="PLZ72_14130"
     CDS             804..>1582
                     /locus_tag="PLZ72_14130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA37482.1"
                     /translation="MTAALSDLRVVEMGQLLAGPFCGQLLADFGADVIKLEPPGQGDP
                     MREWGREKADGRSLWWPVVARGKRSVTINLREKDGQDLARRLIATADVVIENFRPGTL
                     EKWGMAPEDLMADNPRLIVVRVSGYGQDGPYASRPGYGAIGEAMGGLRAVIGEPDRPP
                     ARAGISIGDTLAATFACVGTLVALHARENTGRGQVVDAALYEAVLGVMESLIPEYTVS
                     GYVRPRTGSILPNVAPANAYPTADGEHLISGNQDTVFKRLA"
     assembly_gap    1583..1682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1683..1864
                     /locus_tag="PLZ72_14135"
     CDS             <1683..1864
                     /locus_tag="PLZ72_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA37483.1"
                     /translation="AHPQLGDFPMQNVVPKLSDTPGEVRWVGPELGEHTDEVLAEALD
                     LDEAARSALRDAGVI"
     gene            2056..3198
                     /gene="sucC"
                     /locus_tag="PLZ72_14140"
     CDS             2056..3198
                     /gene="sucC"
                     /locus_tag="PLZ72_14140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNA37484.1"
                     /translation="MDLFEYQGKQFFATFDIPVSAGEAVSTVDDAVAVAERLNSYPVV
                     IKAQVHVGGRGKAGGVKLATNADEVREHASNILGLDIKGHVVKIIWVEVASDIDEEYY
                     ASFTLDRSAKKHLGMLSAQGGVEIEAVAESDPDAIAKIWIDPVLGLTDEVARDWVAAA
                     ELNPAATEGAVDILKKLYTAYTEGDADLTEINPLILKPTGEVHALDAKVTLDDNAGFR
                     HEWAEYEATQVRDEREAAAHDKGLQYVGLDGTVGIIANGAGLAMSTLDVVNQAGGKPA
                     NFLDIGGGANADVMAGALEVINNDPSVKAIFINIFGGITRGEEVANGIVEALSRVTID
                     SPIVIRLDGTNAEEGRAILQPHLSDKLQMQPTMLEAAAKVVELAGA"
     gene            3241..4140
                     /gene="sucD"
                     /locus_tag="PLZ72_14145"
     CDS             3241..4140
                     /gene="sucD"
                     /locus_tag="PLZ72_14145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNA37485.1"
                     /translation="MSIFVDENTKVVYQGLTGSQGRYYGLLNRDYGTQVVAGTNPKKA
                     GTDVDGIPIFASVADAVAETGANASCIFIPAPGVRSAVMEAAEGGVEFIVCITEGVPM
                     QDEAWFFNKLQRDFPSVQLLGPNCPGIISPGKCNIGITAGHIAKAPVAGERNVGIVSR
                     SGTLTYQALYELKLQDIGVTTCVGIGGDPVPGTSFIDCLERFEADPDTSAVMMIGEIG
                     GSAEEEAADFIASKMTKPVVAYIAGQTAPAGKKMGHAGAIVSGGKGTAAAKMEALGSA
                     GVQVGANPTEAGSLMVDVVRSLA"
     gene            complement(4214..5137)
                     /locus_tag="PLZ72_14150"
     CDS             complement(4214..5137)
                     /locus_tag="PLZ72_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNA37486.1"
                     /translation="MGTGSDDHLYAWAAAHHGLYRTDDALRLGLSRRQIAYRVQQGRA
                     ERVGTSVYRVVGSAPTRDQRVLAAAWRARGVASFRTAAEMSGLIDSRGGRPHVLVVRT
                     NGHVHDDAIVHRTQDLLAGEVTSIRRIPVTTPARTLVDIGLTITDLDLEHMLHQAIHR
                     RLTTLEEVADTYRRISRRGRNGAGPIRDLLDAYGSGSPAESKLEVLILRILREHGVPP
                     PVRQHPVTADGHDFRLDLAYPTHRVFLEGDGFGVHGGRTPFEDDRWRQDLLVLQGWWP
                     IRFTWRQARERPEWCADVVGRKLAGVENDRK"
BASE COUNT          819 a         1850 c         1845 g          749 t
ORIGIN      
        1 cggcacccgc aagatccgtc gcgaccacct cgtcccgttg ttcgacgccg actcgcagaa
       61 atttgggtcc tgagcgacac accttgtcgc tttccgcgcc ccagaaatcg gaagtcgggc
      121 gtcagttgcc gctggtggcg atggtggtgg tcgtcgtgcg cgactgatcg ggtcgcaggt
      181 cgatgatcag gcgctcgtcc ttgtcgatct cccacgggaa cgcctcgagc tcggacccgt
      241 cggacccgtc ggttccgtcg gcgctccagg tgacgtcgcg gcagcgctcg ccgagcgcgt
      301 cgcggccctc ggccgggtcc gccgcccgac gctcctcgaa gagctccgac tcggggttcc
      361 aggtcacgaa gcagccctcg gcggccccgg gcgcgatggc cgacagcgcg aaccagccgt
      421 ccttgtcgtc ggatccgatg tgcaccacgt agatcggctg gcgctgaccc cggcccgaca
      481 cgtcggagaa cagcagcggc ccgtccttgt cgatctggtc ggccatgcgg gtggcgtaac
      541 cggcgtcgaa ggtgtcgtcg cccaactgga ccttgaccgg gccctgaccg ccggaggcca
      601 ggcggatcat cccccacgct ccgagcacgg cgatgaccag ggcgaccacg gcgatgatcg
      661 cggaccgggt ctgctgggag ccgctgcggc gttcgacggg catgggcgac cagtatcgcc
      721 cggggtgcgg tccgtcagga actcgactcg gtgggcggta cggtctggtc gaccccggcc
      781 atctccgacc cgagaggacc accatgaccg cagccctgtc cgacctccga gtggtggaga
      841 tgggccagct gctcgccgga ccgttctgcg gtcagctcct ggcggacttc ggcgcggacg
      901 tcatcaagct cgagccgccg ggccagggcg accccatgcg ggagtggggt cgggagaagg
      961 ccgacggccg gtcgctgtgg tggccggtgg tggcccgggg caagcggtcg gtcaccatca
     1021 acctgcggga gaaggacggc caggacctgg cccgacggct gatcgccacc gcggacgtcg
     1081 tcatcgagaa cttccggccg ggcaccctcg agaagtgggg gatggcgccc gaggacctga
     1141 tggccgacaa cccgcgcctg atcgtggtgc gggtgtcggg ctacggccag gacgggccgt
     1201 acgcgtcccg gccgggctac ggcgcgatcg gcgaggcgat gggcgggctg cgggcggtga
     1261 tcggcgagcc ggaccggccg ccggcgcgcg ccggcatctc gatcggcgac accctggcgg
     1321 ccaccttcgc ctgcgtcggc acgctggtcg ccctgcacgc ccgggagaac accggtcggg
     1381 gtcaggtggt cgacgccgcg ctctacgagg cggtgctcgg cgtgatggag tcgctgatcc
     1441 ccgagtacac ggtcagcggg tacgtccggc cccgcaccgg gtcgatcctg cccaacgtgg
     1501 cgccggccaa cgcctatccg accgcggacg gcgagcacct gatcagcggc aaccaggaca
     1561 cggtgttcaa gcggttggcc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nntggcgcac ccgcagctcg gcgacttccc catgcagaac gtggtgccca agctgtcgga
     1741 cacgcccgga gaggtgcgct gggtcgggcc cgagctcggc gagcacaccg acgaggtcct
     1801 ggccgaggcg ctcgacctgg acgaggccgc ccgctccgct ctccgggacg ccggggtgat
     1861 ctgagcgccg gtcggcagga ccgccgatcc gccaacggcg ccggtccgcc aacgccgccg
     1921 ggccgcagga gcgccgggcc ggaatggggg cggttcggtc cgtcggtcta cgctcgcccg
     1981 tcggacccgg cgtgacctga cgatccgtca gctcggctcc gggaccgcag agtccgcgac
     2041 aggaaggtgc agagggtgga cctcttcgag taccagggga agcagttctt cgccacgttc
     2101 gacatcccgg tctcggccgg cgaggcggtc agcaccgtcg acgacgccgt ggcggtggcc
     2161 gagcggctga acagctaccc ggtcgtcatc aaggcgcagg tccacgtggg cggccgcggc
     2221 aaggccggcg gcgtcaagct cgccaccaac gccgacgagg tccgtgagca cgcctcgaac
     2281 atcctcggcc tcgacatcaa gggccacgtc gtcaagatca tctgggtcga ggtcgcctcc
     2341 gacatcgacg aggagtacta cgccagcttc accctggacc gctcggccaa gaagcacctc
     2401 ggcatgctgt ccgcccaggg cggcgtggag atcgaggccg tggccgagtc ggaccccgac
     2461 gccatcgcca agatctggat cgacccggtg ctcggtctga ccgatgaggt cgcccgggac
     2521 tgggtcgcgg cggccgagct caacccggcc gccaccgagg gcgcggtcga catcctcaag
     2581 aagctctaca ccgcctacac cgagggcgac gccgatctca ccgagatcaa cccgctgatc
     2641 ctcaagccga ccggcgaggt gcacgccctc gacgccaagg tcaccctcga cgacaacgcc
     2701 gggttccgcc acgagtgggc cgagtacgag gccacccagg tgcgcgacga gcgcgaggcc
     2761 gcggcgcacg acaagggcct gcagtacgtc ggcctcgacg gcaccgtcgg catcatcgcc
     2821 aacggcgccg ggctcgccat gtcgaccctc gacgtcgtca accaggccgg cggcaagccc
     2881 gccaacttcc tcgacatcgg cggcggcgcc aacgcggacg tcatggccgg cgccctcgag
     2941 gtcatcaaca acgacccgtc ggtgaaggcg atcttcatca acatcttcgg cggcatcacc
     3001 cgcggcgaag aggtcgccaa cggcatcgtc gaggcgctga gccgggtcac catcgactcg
     3061 ccgatcgtca tccgcctcga cggcaccaac gccgaggagg gtcgggcgat cctccagccg
     3121 cacctgtccg acaagctgca gatgcagccc accatgctcg aggcggccgc caaggtcgtc
     3181 gagctggccg gggcctgagg cgatcagcgg actgacgaga gggactgagg agacaccacc
     3241 atgagcatct tcgtcgacga gaacaccaag gtcgtctacc agggcctgac cggcagccag
     3301 ggccgctact acggcctgct gaaccgggac tacggcacgc aggtcgtggc cggcaccaac
     3361 cccaagaagg ccggcaccga cgtggacggc atcccgatct tcgcctcggt ggccgacgcc
     3421 gtggccgaga ccggcgccaa cgccagctgc atcttcatcc ccgccccggg cgtgcgctcg
     3481 gcggtgatgg aggcggccga gggcggcgtc gagttcatcg tgtgcatcac cgagggcgtc
     3541 cccatgcagg acgaggcgtg gttcttcaac aagctgcagc gggacttccc gtcggtgcag
     3601 ctcctcggcc cgaactgccc cggcatcatc agccccggca agtgcaacat cggcatcacc
     3661 gccggccaca tcgccaaggc gccggtcgcc ggtgagcgca acgtcggcat cgtgagccgc
     3721 tcgggcacgc tgacctacca ggcgctctac gagctgaagc tgcaggacat cggcgtgacc
     3781 acctgcgtcg gcatcggcgg cgacccggtc ccgggcacct cgttcatcga ctgcctcgag
     3841 cgcttcgagg ccgaccccga cacgtccgcc gtcatgatga tcggcgagat cggcggctcg
     3901 gccgaggagg aggccgccga cttcatcgcc tccaagatga ccaagccggt cgtcgcctac
     3961 atcgccggac agaccgctcc ggcgggcaag aagatgggcc acgccggcgc gatcgtgtcg
     4021 ggcggcaagg ggaccgcggc ggccaagatg gaggcgctgg gttcggccgg cgtgcaggtg
     4081 ggtgcgaacc cgaccgaggc gggcagcctc atggtcgacg tcgtccgctc cctcgcctga
     4141 cgcacccctc tgaaaactgg gcgcggaatc gacacatcct gtcgattccg cgccccagat
     4201 ccggtcgctg gggtcatttc cggtcgttct cgacgccggc gagcttgcgg ccgaccacgt
     4261 ccgcgcacca ttccggtcgt tcgcgggcct gacgccacgt gaaccggatc ggccaccatc
     4321 cctgtaggac gagcaggtcc tggcgccatc ggtcgtcctc gaacggagtg cggccgccgt
     4381 ggaccccgaa cccgtcaccc tccaggaaca ccctgtgggt cggatacgcc aggtccaacc
     4441 ggaagtcgtg accgtcggcg gtgacgggat gctggcgcac cggtggaggg actccgtgtt
     4501 cccgaaggat ccgcaggatg agcacctcca acttgctctc ggcgggtgac ccagagccgt
     4561 aggcgtcgag caggtcgcgt atcggacccg cgccgttgcg gcctcggcga gagatgcgcc
     4621 ggtaggtgtc ggcgacctcc tccagggtgg tgagccgacg gtggatggcc tggtgtagca
     4681 tgtgctcgag gtccaggtcc gtgatcgtga ggccgatgtc gaccagggtc cgcgccggcg
     4741 tggtcaccgg gatccgacga atgctggtca cctcgccggc cagcaggtcc tgggtgcggt
     4801 ggacgatggc gtcgtcgtgg acgtggccgt tggtccggac gaccagcacg tgcgggcgtc
     4861 ctccccgcga gtcgatcagc cctgacatct cggcagcggt gcggaacgat gcgacgcccc
     4921 gggcacgcca ggcggcggcg agcacccgct ggtcccgggt gggggccgaa cccacgaccc
     4981 ggtacacgct cgtgccgacc cgctcggccc gtccctgctg cacccggtac gcgatctggc
     5041 ggcggctcag tcccagtcgg agggcgtcgt cggtgcggta gagcccgtgg tgtgccgccg
     5101 cccaggcgta gaggtgatcg tcgctgcccg ttcccatccg aacgagcaga cactgcgacc
     5161 cgggtgcttg tcagcgggtc gcgcgacccg ttgacacccc gagcgggatg cgtactcggt
     5221 cgccttcgaa tctggggcgc ggaatcgaca ggacgtgtcg attgcgaccc ctatgttctg
     5281 ggtgggggtg ggcgctggta atttcggccc gtcatgcgac tcgccgtcct cgcctccggt
     5341 tcgggcaccc tcctcgacgc cat
//
LOCUS       DAOQOD010000197         5260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6416_length_5260_cov_12.806916, whole genome shotgun sequence.
ACCESSION   DAOQOD010000197 DAOQOD010000000
VERSION     DAOQOD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6416_length_5260_cov_12.806916"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..463)
                     /gene="dut"
                     /locus_tag="PLZ72_14155"
     CDS             complement(29..463)
                     /gene="dut"
                     /locus_tag="PLZ72_14155"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNA37487.1"
                     /translation="MLEVPVVRLDPELPLPRYARPGDAGVDLVAATDLELAPGGRGLV
                     PTGVAVAIPRGYAGFIQPRSGLALRHGVTVLNTPGLIDADYRGELKVLLVNLDPAETF
                     LVTRGERIAQLVVQPVEHVRFVETDELDDTDRGEGGFGHTGR"
     gene            550..1158
                     /gene="orn"
                     /locus_tag="PLZ72_14160"
     CDS             550..1158
                     /gene="orn"
                     /locus_tag="PLZ72_14160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNA37488.1"
                     /translation="MLVWMDLEMTGLDPARNVIVEIATLVTDDDLNIVAEGPDLVVHT
                     TEDELAKMEPIVVDMHTRSGLLDEIRSSTLTLEEAGAQTLEFIRTHVPEPRTVPLCGN
                     SIGMDRRFLAIHLPEIEEHLHYRSIDVSTIKELARRWYPGSLDGLTKKSSAHRAMDDI
                     KESVAELVHWRNAVFVPSPTPETPAPVTPAPAPDDAGTAAGA"
     gene            1158..2285
                     /locus_tag="PLZ72_14165"
     CDS             1158..2285
                     /locus_tag="PLZ72_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNA37489.1"
                     /translation="MVERQERLPARDEVDEVAPGIGRIQLPIMIPGLGHVNCYVLEDE
                     RGVALVDPGMPDPWTHQVLIERLRSIDIAPEHIHTVLVTHSHPDHFGGAGRLRVEHRC
                     EVVAHRRFHTPFDPSPDPFADDLTELTVATDPLEPGGDDDRPRNERLAEVLFGEGPMP
                     EVPPRPTPWGEEGFKPGPGELEAMRAWDDLSKQGLLRLDPTVRVDDLEVLRLGGREWQ
                     VVHTPGHTGDHICLLDPADGTFLSGDHVLPTITPHISGMTGDDDPLAEFVAALHRVAA
                     LDVVTRVLPAHGLPFDDLAGRVDDIIGHHVERIAQLERLGQELGDATVRDYSRRLFQE
                     RSWGPMAESETYAHLLHLQHEGRMAVRTDEDARLRYRPVPA"
     gene            2471..2650
                     /locus_tag="PLZ72_14170"
     CDS             2471..2650
                     /locus_tag="PLZ72_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37490.1"
                     /translation="MSTTTTFHAEHAMWRSDVVRARLHLCPTGNRFGIANEYGTTPAA
                     TKPATATARRGAPPP"
     gene            2857..3243
                     /locus_tag="PLZ72_14175"
     CDS             2857..3243
                     /locus_tag="PLZ72_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HNA37491.1"
                     /translation="MTIVDTRPRPSGSALAAAPGVLDRSEWETDALCRDLSTELFFSD
                     DVDEIGDAKRICLSCPVRTRCLDAAVERGEQFGIWGGHLFVSGRIVLHKRRRGRPPKV
                     ERPGDRFPEVEVPEPYRRLVRAGSPG"
     gene            3387..4052
                     /locus_tag="PLZ72_14180"
     CDS             3387..4052
                     /locus_tag="PLZ72_14180"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNA37492.1"
                     /translation="MTRTDETTAEQPADDPAQPGRRRRSGLPIGPLGLFLATAIALAA
                     AGLVLWIGTRSDSDAIPVEKALEQIDGGSLVPAGTDPATVGSPAPDVRLNLLDGTTTR
                     LSAFQGRPVVLNFWSSTCAPCLKEMPDLERVWTSHSGQVAVVGVDVTDTEAAGREMVE
                     QTGVTYPNGRDPGADIFAAFGGTALPRTVFIDAKGTVVEAHNGALTSDEVDGILRDRG
                     LLG"
     gene            4084..5022
                     /locus_tag="PLZ72_14185"
     CDS             4084..5022
                     /locus_tag="PLZ72_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNA37493.1"
                     /translation="MGARLALAFSAGMVATVNPCGFALLPAYLTYFLGLDPAGSDATT
                     GSADARRSDPSPVLRALVVSAAVTAGFLVVFGVMGLAWSSVSSVIGRRLPWFTLVIGV
                     GLVVLGVAVLRGFEPTLRLPHLDLNRSGRELVTMFLFGISYAVASLSCTIPVFISLVS
                     VTLDGSFGQSVGAFVAYGLGMGMTLAILTVAVALARTGVVTAFRRLMPHMAAISGALL
                     IVAGLFVGYYAWVELRELDSGASSPVVDWTRDVQSWLQNHVERIGGARLAVAAALVIG
                     AVVAAALISRRGADRPPGDARDHVGPPDRGRESDRS"
     gene            5032..>5260
                     /locus_tag="PLZ72_14190"
     CDS             5032..>5260
                     /locus_tag="PLZ72_14190"
                     /inference="COORDINATES: protein motif:HMM:NF037471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37494.1"
                     /translation="MPVLTRLRSQFPDAMPIGTYLDRVATALDPFDFRPDGTFAAVSI
                     CRDELTQQLIEQVAQRWHRPFALGGLGGLPSL"
BASE COUNT          754 a         2024 c         1755 g          727 t
ORIGIN      
        1 gcagccagaa cccgcacggc tgctgcagtc agcggccggt gtggccgaag ccgccctcgc
       61 cccggtcggt gtcgtccagc tcgtcggtct cgacgaaacg gacgtgctcg accggctgca
      121 ccaccagctg agcgatgcgc tcgccccggg tgaccaggaa cgtctcggcc ggatcgaggt
      181 tgaccagcag caccttgagc tcaccccggt agtcggcatc gatcagcccg ggggtgttga
      241 gcacggtcac gccgtggcgg agggccagac cgctgcgcgg ctggatgaac ccggcgtagc
      301 cgcgggggat cgccaccgcg acgccggtgg ggaccaggcc ccgcccgccc ggcgccagct
      361 ccagatcggt cgcggccacc aggtcgaccc cggcgtcgcc cgggcgggcg tagcggggca
      421 acgggagttc gggatcgagt cggacgacgg gcacctcgag cacggccgga ccctaccggg
      481 accgctgtcg gcgtgccgag cccgggcacc ggtgggcgct tccgtcgacg gtcaccgtag
      541 ggtgggacgg tgctcgtctg gatggacctg gagatgaccg gcctggaccc ggcccgcaac
      601 gtgatcgtcg agatcgccac cctggtgacc gacgacgacc tgaacatcgt cgccgagggc
      661 ccggacctgg tcgtccacac caccgaggac gagctcgcca agatggagcc catcgtcgtc
      721 gacatgcaca cccgcagcgg tctactcgac gagatccgct cctccaccct gaccctcgag
      781 gaggcgggcg cacagacgct cgagttcatc cgcacccacg tgcccgaacc ccggaccgtc
      841 cccttgtgcg gcaactcgat cggcatggac cgccgcttcc tggcgatcca cctgcccgag
      901 atcgaggagc acctgcacta ccgctcgatc gacgtgtcga ccatcaagga attggcccgc
      961 cgctggtacc cgggatccct ggacggtctg accaagaagt cgtccgcgca ccgggccatg
     1021 gacgacatca aggagtcggt cgccgagctg gtgcactggc gcaacgccgt gttcgtaccg
     1081 tcccccaccc ccgagacacc cgcgcctgtg acacccgcgc ccgctcccga cgacgcgggc
     1141 acggccgccg gggcctgatg gtcgaacgac aggaacgtct gccggcccgc gacgaggtcg
     1201 acgaggtcgc gccggggatc gggcgcatcc agctcccgat catgatcccg ggactgggtc
     1261 acgtgaactg ctacgtcctc gaggacgaac ggggcgtcgc cctggtcgac ccgggcatgc
     1321 cggacccgtg gacccaccag gtgctcatcg aacgtctgcg ctcgatcgac atcgcgcccg
     1381 agcacatcca caccgtgctg gtcacccact cccatccgga ccacttcggc ggggcagggc
     1441 ggctcagggt cgaacaccgc tgcgaggtgg tcgcccaccg tcgcttccac accccgttcg
     1501 acccgtcgcc ggatccgttc gccgacgacc tgaccgagct gaccgtggcg accgacccgc
     1561 tggagcccgg cggggacgac gaccgacccc gcaacgagcg tctggccgag gtcctcttcg
     1621 gcgaggggcc gatgcctgag gtgccgcccc gtccgacgcc gtggggcgag gaggggttca
     1681 agcccggccc cggagagctc gaggcgatgc gggcctggga cgacctgtcc aagcaggggt
     1741 tgctgcgcct ggacccgacc gtccgggtcg acgatctgga ggtgctgcgc ctgggcggcc
     1801 gcgagtggca ggtcgtgcac acccccggcc acaccgggga ccacatctgc ctcctcgacc
     1861 cggccgacgg cacgttcctg tccggcgacc acgtgctgcc caccatcacc ccccacatct
     1921 cgggcatgac cggcgacgac gacccgctgg cggagttcgt cgccgcgctg caccgcgtcg
     1981 cggccctcga cgtggtgacc cgggtgctcc ccgcgcacgg actgcccttc gacgacctcg
     2041 ccggccgcgt cgacgacatc atcggtcacc acgtcgagcg catcgcccag ctcgaacggc
     2101 tgggccagga gctgggcgac gccaccgtcc gggactactc ccgccggctg ttccaggagc
     2161 ggtcgtgggg cccgatggcc gagtcggaga cctacgccca cctcctgcac ctccagcacg
     2221 agggccggat ggcggtccgc accgatgagg acgcccggct gcgctaccgc cccgtccccg
     2281 cctgacgcca gaccccggtc cgggccgatc cgggccgatc cgggccgatc cgggccgatc
     2341 cgacagatct ccggaaattc gcttgaccgc cccccgaggg ccgtggttcg ctcgacccac
     2401 gagccggtac ggccgggatc tccggccgca ccggcggcga acgacaccag actccaacca
     2461 gagcagaccg atgagcacca ccacgacctt ccacgccgag cacgccatgt ggcgctccga
     2521 cgtggtgcgt gcgcgtctgc atctctgccc gaccggcaac cgattcggga ttgcgaacga
     2581 gtacggcacc acgccggccg ccaccaagcc ggccactgcc accgcccgac gaggagcccc
     2641 tccgccctga cccagaggtc acagcgcagg aaggccccag agccgccggg tggaactcca
     2701 ccgggcggct cttgcgcttt tcggcgtcgg ggtctcacca ccagcccgct gcccgaacca
     2761 cagccgcccg aaccacagcc gctcgaacca cagccgccag tcctaccgac caccacctcg
     2821 acccaccgcc accccgtccg aaccaggaga tccaccatga ccatcgtcga cacccgcccc
     2881 cgaccctcgg gcagcgccct ggccgccgca ccgggagtgc tcgaccgctc cgagtgggag
     2941 accgacgccc tgtgtcgcga cctgagcacc gagctgttct tcagcgacga cgtcgacgag
     3001 atcggcgacg ccaaacgcat ctgcctcagc tgtccggtcc ggacccgatg tctggacgcc
     3061 gcggtcgaac gcggcgagca gttcggaatc tggggtgggc acctgttcgt gtccggccgg
     3121 atcgtcctgc acaagaggcg tcggggacgg ccgcccaagg tcgaacgacc cggcgaccgc
     3181 ttccccgagg tcgaggtccc cgagccgtac cggcgcctgg ttcgcgccgg atctcccggc
     3241 tgaacggaac cgagtcctcc ggacccgacg gacggtgacg caccgaccgc gagctgaccg
     3301 atgaggtccg gatcactaca gtggaggggc cctcgcgggc ccctccaccg gagcccgccc
     3361 gtccccccgg ccaggatccc cgcaccatga cgcggaccga cgagaccacc gccgaacagc
     3421 ccgccgacga cccggctcag cccggacgcc ggcgccgctc cgggctgccg atcgggccgc
     3481 tcgggctgtt cctggccacc gccatcgccc tggccgccgc cggactcgtc ctgtggatcg
     3541 gcacccgatc cgacagcgac gccatcccgg tcgagaaggc cctcgagcag atcgacggcg
     3601 gatcgctcgt ccccgcaggc accgaccccg ccaccgtcgg ttcgccggcc ccggacgtgc
     3661 gcctgaacct cctcgacggc accacgaccc ggctgtcggc gttccagggc cgcccggtag
     3721 tgctgaactt ctggtcctcg acctgcgcgc cgtgcctcaa ggagatgccg gatctggaac
     3781 gggtgtggac gtcgcactcc ggacaggtgg ccgtcgtggg tgtcgacgtg accgacaccg
     3841 aggcagccgg gcgcgagatg gtcgagcaga ccggggtgac ctaccccaac ggacgcgacc
     3901 ccggcgccga catcttcgcc gccttcgggg ggaccgccct gccccgcacc gtgttcatcg
     3961 acgcgaaggg caccgtggtc gaggcccaca acggcgcgct cacctccgac gaggtcgacg
     4021 gcatcctccg cgaccggggc ctgctcggct gagcggccgg cgccggacag gatctccgaa
     4081 cccgtgggcg cacgcctggc gctggccttc tccgccggga tggtcgccac ggtcaacccg
     4141 tgcggcttcg ccctgctccc ggcgtacctg acctacttcc tggggctcga ccccgccggg
     4201 tcggatgcga cgaccggctc cgccgacgct cgccgttccg atcccagccc ggtgctgcgg
     4261 gcgctcgtgg tgtcggccgc ggtgaccgcc gggttcctgg tcgtgttcgg ggtgatgggc
     4321 ctggcctggt cgtcggtgtc gtctgtcatc gggcgtcgcc tgccctggtt cacgctggtg
     4381 atcggcgtcg gcctggtcgt cctgggcgtg gcggtgctcc gcgggttcga gcccaccctg
     4441 cgactgccgc acctggacct caaccgcagc gggcgcgaac tcgtcaccat gttcctgttc
     4501 ggcatctcct acgccgtcgc gtcgttgagc tgcacgatcc cggtgttcat ctcgctggtg
     4561 tcggtcaccc tcgacggcag cttcggccag agcgtcgggg cgttcgtcgc ctacgggctg
     4621 gggatgggga tgacgctcgc catcctgacc gtggccgtcg ccctggcccg caccggggtg
     4681 gtcaccgcgt tccgacggct gatgccgcac atggccgcca tctccggagc gctgctcatc
     4741 gtggccggcc tcttcgtcgg ctactacgcc tgggtcgagc tgcgcgagct cgactcgggc
     4801 gcctcgtcgc cggtcgtcga ctggacccgc gacgttcaga gctggctgca gaaccacgtg
     4861 gagcggatcg gcggtgcccg cctggccgtc gccgcagctc tggtcatcgg ggcggtcgtc
     4921 gccgcggcgt tgatcagccg ccgtggcgcg gaccgacccc cgggcgacgc gcgggaccac
     4981 gtcggcccac ccgatcgggg tcgggaatcg gatcggtcct agggtgaccg ggtgcccgtg
     5041 ctgacccgcc tccgctcgca gttccccgac gccatgccga tcggcacgta cctggaccgg
     5101 gtcgccaccg ccctcgaccc gttcgacttc cgacccgacg ggaccttcgc ggcggtgtcg
     5161 atctgccgcg acgagctgac ccagcagctg atcgagcagg tcgcgcagcg ctggcaccgc
     5221 ccgttcgccc tcggcggtct gggcggcctg ccgtcgctcg
//
LOCUS       DAOQOD010000198         5221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6497_length_5221_cov_8.446767, whole genome shotgun sequence.
ACCESSION   DAOQOD010000198 DAOQOD010000000
VERSION     DAOQOD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_5221_cov_8.446767"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLZ72_14195"
     CDS             <1..279
                     /locus_tag="PLZ72_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNA37495.1"
                     /translation="VDRTIEVTELAAARVRASAHLELVMEPELSVLLVRRPGWTPQRY
                     RDHCERLARDQVGFVVPTTWRGETVLRLCVVNPVTTPDDVTAVLPPPP"
     gene            complement(302..1087)
                     /locus_tag="PLZ72_14200"
     CDS             complement(302..1087)
                     /locus_tag="PLZ72_14200"
                     /inference="COORDINATES: protein motif:HMM:NF017135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HNA37496.1"
                     /translation="MTEPSRTHLVVRRLTDAAQLRALLHREPVNPVDDLDDPDAVEQA
                     LGVRTGSDRRVFGLVHPEHPERVRTVVWVALTHGVPDSLVPLTDGAPSSAAGPDVDSA
                     DTAVFWSIWNDDRTVEGVGRGGDLIEGAAELLRAEHPGLSTMVTLSPAPGLRDWANAP
                     ERVEAAPDPLDHAAVATAAARYLTAQDETGRPIDPVARFHLGNGARLWRVVPDADPSP
                     RGRSRSFGLLVNYRYEPEDRAANRAELDAGRIALGDQVRELLG"
     gene            1214..2335
                     /locus_tag="PLZ72_14205"
     CDS             1214..2335
                     /locus_tag="PLZ72_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNA37497.1"
                     /translation="MRTHLRLGRLLGVPIGINGGVLLVSVLLAISLAQVSLPSIAPRH
                     PTSAYWFGAVLGVVGFLVSLVGHELGHSYIAQRNGVRVAEITLWLFGGVAKLEGDADE
                     PGAEFRIAAAGPAMSMVIAVLAGLSAWEIDQLDGSRVLLGLLVWLSAINVILAVSNLL
                     PAFPLDGGRMLRAFLWRRSGRKRRATRTAAMIGQVLAVVILIAAVVLFFPMGDRWSGA
                     WILALGLFLFVAARTQWRDAADRPEVLEHPVGELRRELPAPLSPRASVAELEAVLRTN
                     PRAVFVPVLDERGAVGALVTRDAVARIPPANRASVPIRSLAEPLVALPRVHPAESVQD
                     VVGRLGRGRSWWALVVDGAGVEGVLCSEDVDDVLDVAFA"
     gene            2473..>5221
                     /locus_tag="PLZ72_14210"
     CDS             2473..>5221
                     /locus_tag="PLZ72_14210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37498.1"
                     /translation="MLSALLRQRRRLLLATVAIALSVGYLAGALNLLDRVGRGLDDLA
                     AAGSAPADLVIEGGIAYESPTEQVRRLVPASLEATVAQVPGVAVVSPRIEDTAPIVGP
                     DGKTLVRLGLTEQPIGANFPADARLSPYEFVSGGPPTTSTEVAIDRRSAAEGGVEVGD
                     QVFVVGRAGAAPYTVSGIVSTDGGGLPPGSSLALLSTAEARVRFDRPTDDNTIDILVE
                     PGADVAQVRSSIEALLPAGIEVVDGPTGAEHRQESVDRSFTLVRSLILGFAALALVVG
                     MVTVGNSLTLLYAERRRTFAGLRLVGAKPRQLLFAALAEAALLALLASVLGAPLGLLL
                     GRLIEGALGALNTSVPVAGSTVSIPALVWAVVIGTIATVVAAVVPAVRACRVPPIEAV
                     GHGASPVPRTGAQSLVRTAIVAVVVGLAAALLVSRGEAGLNPVSLGLGVAVAVMVLGS
                     TPLLLAGLVSGFVGVLPLRPPALRTIAARDVARHRSRTAATAAALILATAVVSGLAVF
                     LSSFSHSVDGQVRDLVRADLVVDSGTFTKGGLPEDLVAQISGLEPVTATSGWQIGRGW
                     VGARPVRMTGFDMARFGELMNPTWVGPAPGPLDESSVLVSSRLADELGLQVGASVPVT
                     FTSTGAENLRVAGVYRSGELLLGDMVLDRAVLSRQVPATTDIAALVALSPDDAATRAQ
                     VEELASRSGVSSVLRPDAFVERRSEVLTGFQRVIEWMLLFTLVQALIGVVNTLLLSVG
                     ERRRELGLLRASGATRRQVLRMVLGEGVALALVGTFVGLVVGLVGARLGVAALASLGV
                     TSFAVPVGGVALVGVAAATLGVLATVAPARWASAVPPLEAVFDDGDLRSRPRRAQRPR
                     LHRMGKAAFHWTGDADLITDPATPPSAAFPPLPTGRGVQLPTVPPPLPPHMVIPPPPA
                     TS"
BASE COUNT          554 a         1868 c         1970 g          829 t
ORIGIN      
        1 gtcgaccgca cgatcgaggt caccgagctc gccgcggccc gggtccgggc gtcggcgcat
       61 ctggagctgg tcatggaacc cgagctgtcg gtgctcctgg tccgtcggcc cgggtggacc
      121 ccgcagcgct accgcgacca ctgcgagcgg ttggcccgtg accaggtggg cttcgtcgtg
      181 ccgacgacgt ggcggggtga gacggtgctg cggctgtgcg tggtcaaccc ggtcacgacg
      241 cccgacgacg tcaccgccgt gctacctccg ccgccgtgag gggagggtcc cgcgttcggg
      301 ttcagccgag cagctcgcgg acctggtcgc cgagggcgat ccgtccggcg tcgagttcgg
      361 ctcggttggc ggcgcggtcc tcgggttcgt agcggtagtt gaccagcagg ccgaaggagc
      421 gggagcggcc ccgcggtgac ggatcggcat ccggaacgac cctccacaac cgggcgccgt
      481 tgcccaggtg gaaccgggcg acgggatcga tcggtcggcc cgtctcgtcc tgtgcggtca
      541 ggtagcgcgc cgcggcggtg gcgacggcgg cgtgatcgag cgggtccggc gcggcctcga
      601 cccgctcggg ggcgttcgcc cagtcccgca gtccgggagc cggtgacagg gtgaccatgg
      661 tcgacaggcc cgggtgctcg gcgcggagca gctcggcggc gccctcgatc agatccccac
      721 ccctgccgac cccctcgacg gtccggtcgt cgttccagat cgaccagaac accgccgtgt
      781 cggcgctgtc cacgtccggg ccggcggccg acgatggggc cccgtcggtg agcgggacca
      841 gcgagtcggg cacgccgtgg gtcagtgcga cccacacgac ggtgcggacc cgttcggggt
      901 gctccgggtg caccaggccg aacacccgcc ggtccgatcc ggtgcgcacg cccagcgcct
      961 gctccacggc gtcggggtcg tcgaggtcgt cgacggggtt caccggttca cggtgcagca
     1021 gcgcccgcag ctgggcggcg tcggtcaagc gccggacgac caggtgggtg cgggagggtt
     1081 cggtcacggg attgtccggg tcgatcggtc gcagggtcac aggcagcagg cgtccaccgg
     1141 cccgctgacc ggcctctcat ccgagcatcc cggcggggac ggtgccaaca cgtcagccgg
     1201 taccctgccc gttgtgcgca cccacctccg actcggccgg ctgctcggcg tccccatcgg
     1261 gatcaacggc ggtgtgctgc tggtcagcgt cctgctggcg atcagcctgg cgcaggtgtc
     1321 gctgccgagc atcgcgcccc gccaccccac cagcgcctac tggttcggcg ccgtcctggg
     1381 cgtggtcggg ttcctggtgt cgttggtcgg ccacgagctc gggcacagct acatcgccca
     1441 gcgcaacggc gtgcgagtgg ccgagatcac gctgtggctc ttcggcggcg tcgccaagct
     1501 cgagggcgac gcggacgagc ccggggccga gttccggatc gcggcggcag gcccggcgat
     1561 gagcatggtg atcgccgtgc tggccggcct gtcggcgtgg gagatcgacc agctcgacgg
     1621 atcccgggtg ctgctcggcc tgctggtctg gttgtcggcc atcaacgtga tcctggcggt
     1681 gtcgaacctg ctgccggcgt tcccgctcga cgggggccgg atgctgcgcg cgttcctgtg
     1741 gcggcggtcg gggcgcaagc ggcgggcgac ccgcaccgcc gcgatgatcg ggcaggtact
     1801 cgccgtcgtg atcctcatcg ccgcggtcgt gctgttcttc cccatggggg accgctggag
     1861 cggggcgtgg atcctggcgc tcgggctctt cctgttcgtc gccgcccgca cgcagtggcg
     1921 cgacgcggcg gatcgcccgg aggtgctcga gcaccccgtc ggcgagctcc gacgcgagtt
     1981 gcccgccccg ctgtcgccgc gggcgtcggt ggccgagctc gaagcggtcc tcaggaccaa
     2041 tccccgggcg gtattcgtcc cggtgctcga cgagcggggt gcggtcgggg ccctcgtcac
     2101 ccgggacgcg gtcgcccgga tccctccggc caaccgcgcc tcggtgccga tccgctccct
     2161 ggccgagccg ctcgtggcgc ttccgcgcgt ccaccccgcc gagtcggtgc aggacgtggt
     2221 cggtcgtctc ggtcggggtc gctcgtggtg ggctctggtc gtcgacggcg ccggcgtcga
     2281 gggcgtgctg tgctccgagg acgtcgacga cgtcctcgac gtggcgttcg cctgaacgtc
     2341 cgccggcgcg ggtcgagcac cgcgggccgg tagcgtaggg acgtgtccga ggtggtcggt
     2401 cagagctccg gccgatctcc gggccgcccg gctgggcgtc ggggtgggcg tcggggcgtg
     2461 tgggtgcgac cgatgctgtc cgccctgctg cgccagcgtc gccgactgct gctggcgacg
     2521 gtggccatcg ccttgtcggt gggctacctc gccggagcgc tgaacctgct cgatcgcgtc
     2581 ggtcgagggc tggacgacct cgccgctgcc ggctccgcgc cggccgacct cgtcatcgag
     2641 ggcgggatcg cctacgagtc gcccaccgag caggtccgtc ggctggttcc cgcctccctg
     2701 gaggccaccg tcgcccaggt tcccggcgtg gccgtggtgt cgccccgcat cgaggacacc
     2761 gccccgatcg tcggcccgga cggcaagacc ctggtgcgcc tcgggctcac cgaacagccc
     2821 atcggcgcga acttccccgc cgatgctcgg ctgtcgccct acgagttcgt ctccggtggc
     2881 ccaccgacca cgtcgaccga ggtcgcgatc gaccggcgtt ccgccgccga aggcggcgtg
     2941 gaggtcggcg accaggtgtt cgtcgtgggg cgcgccgggg ccgcgcccta caccgtgtcc
     3001 ggcatcgtga gcaccgacgg aggtggtctg ccccccggct ccagcctggc cctgctgagc
     3061 accgcggaag cccgcgtgcg cttcgaccga ccgaccgacg acaacaccat cgacatcctg
     3121 gtcgaaccgg gtgccgacgt tgcccaggtg cgatcgtcga tcgaggcgct gctcccggcc
     3181 ggcatcgagg tggtcgacgg acccaccggg gccgagcacc ggcaggagtc ggtcgaccgc
     3241 agcttcaccc tggtccgctc gttgatcctc ggcttcgccg cgctcgcgct ggtggtcggc
     3301 atggtcaccg tcggcaactc gctgacgctg ctgtacgccg aacgccgacg cacgttcgcc
     3361 gggttgcgac tggtcggggc gaagccgcgc cagctgttgt tcgcggccct ggcggaggcg
     3421 gcgctgctcg cgctgctggc ctcggtgctc ggcgctcccc tcgggctgct gctcggccga
     3481 ctgatcgagg gggccctggg agcgttgaac acgtcggtcc cggtcgccgg gtccaccgtg
     3541 tcgatcccgg cgctggtgtg ggcggtcgtc atcggcacga tcgcgaccgt ggtcgcggcg
     3601 gtggtcccgg cggttcgcgc ctgtcgtgtg ccaccgatcg aggcggtcgg ccacggagct
     3661 tctccagttc cccgcaccgg cgcccagtcg ttggtgcgga cggcgatcgt ggcggtcgtg
     3721 gtcggtctgg ccgcggctct gctggtgtcg cgtggcgaag cgggcctgaa cccggtgtcg
     3781 ctcggcctcg gcgtggcggt cgcggtgatg gtgctcgggt cgaccccgct gctgctcgcc
     3841 ggtctggtgt cgggcttcgt cggcgtgctc cccctgcgtc cacctgcgct gcgcacaatc
     3901 gcggcccgtg acgtcgcccg ccaccgctcc cgcaccgcgg cgacagccgc cgcgctgatc
     3961 ctggccaccg cggtcgtgtc gggcctggcg gtgttcctct cgtcgttcag ccactccgtc
     4021 gacggccagg tcagggacct ggtgcgggcc gacctggtcg tcgactccgg gaccttcacc
     4081 aaaggcggtc tgcccgagga cctggtggcg cagatctcgg gtctggagcc ggtcaccgcg
     4141 acgagcggct ggcagatcgg tcggggttgg gtcggagccc gaccggttcg catgaccggc
     4201 ttcgacatgg cccgcttcgg ggagctgatg aacccgacgt gggtcggccc ggcgcccggc
     4261 cccctcgacg agtcgagcgt gctggtgtcg tcgaggctgg ccgacgagct cgggctgcag
     4321 gtcggcgcct cggtgccggt caccttcacc agcaccggag cggagaacct gagggtcgcc
     4381 ggcgtgtacc gctccggcga gctccttctc ggcgacatgg tgctcgaccg ggccgtgctg
     4441 agccgacagg tccccgccac caccgacatc gcggcgctgg tcgccctgtc gcccgacgat
     4501 gccgccaccc gggcgcaggt cgaggagctg gcgtcgcggt ccggggtgag ctcggtgctg
     4561 cggcccgacg cgttcgtcga gcgtcgatcc gaggtgctga ccgggttcca gcgggtcatc
     4621 gagtggatgc tgctgttcac cctggtccag gcgctgatcg gtgtggtcaa cacgctgctc
     4681 ctgtcggtgg gtgaacgccg tcgcgagctg ggcctgctgc gcgccagcgg tgccacccgc
     4741 cgccaggtgc tgcgcatggt gctcggcgag ggcgtcgccc tggctctcgt cgggacgttc
     4801 gtgggactgg tggtcgggct ggtgggtgca cgcctcggtg tcgcagcgct cgcctcgctg
     4861 ggtgtgacgt cgttcgcggt gccggtcggt ggcgtggcgc tggtcggcgt ggcggcggcg
     4921 accctggggg tcctggcgac cgtcgccccg gcccggtggg cgtccgcggt cccgccgctc
     4981 gaggcggtgt tcgacgacgg cgacctccgg tcccgacccc gacgcgccca gcggccgcgc
     5041 ctgcaccgga tgggcaaggc ggcgttccac tggacgggcg acgcggacct gatcaccgac
     5101 ccggccacgc cgcccagcgc ggcgttcccc ccgctgccga ccgggagggg agtccagctg
     5161 ccgacggttc cgccgccgct gcctccccac atggtgatcc cgcctccacc ggcgacgtcc
     5221 g
//
LOCUS       DAOQOD010000199         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6502_length_5219_cov_12.679512, whole genome shotgun sequence.
ACCESSION   DAOQOD010000199 DAOQOD010000000
VERSION     DAOQOD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6502_length_5219_cov_12.679512"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLZ72_14215"
     CDS             <1..719
                     /locus_tag="PLZ72_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37499.1"
                     /translation="AMVGVLRGVAERAPRLSRPAADAFIADAITAVLRTTPVHAIDPG
                     RLHPSDAVTWRLPPSPRERILAAALPLFRSRGFDGVGIGEIGDHAGVGASNVSRYFGS
                     KEEVLVDIYDRVGARVEVAVDDAVASASDADHALQNLLEAYTEVAFGAADLVVVVGEN
                     RGALPRNEIARLRRRDRRVADIWRGVIAALRPELRPVETTTVVAGVIALVNSFPRHEP
                     DALPAPSAVVPLARAFVGAA"
     gene            complement(747..1103)
                     /locus_tag="PLZ72_14220"
     CDS             complement(747..1103)
                     /locus_tag="PLZ72_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNA37500.1"
                     /translation="MATSTRRLTVRSDDVAECCPALLAEPLDPTDAVELAAVFKALAD
                     PVRLQLLSLIAAAPNATACSCDLEEPVGKSQPTVSHHLSVLADAGLITKEKVGRWVNC
                     TIVADRLAQVRAALES"
     gene            complement(1175..1786)
                     /locus_tag="PLZ72_14225"
     CDS             complement(1175..1786)
                     /locus_tag="PLZ72_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37501.1"
                     /translation="MSKSSRVRLLGAPLLVLGAIGLLASCAPEPPPYRGIVFNAPSYS
                     YIGRTFTPTATDTSGMPVTITLDASSTGCTFVGGVVSFNSLGNCVLNADQPGDATHDP
                     SPRVQRKIAIVPCPPMRPGIWTGPMNLSANVSVSGSLFYGTIDLTSLNYGVQSFSGNV
                     SCEVVDMTFNNVPLRGYLSPDGKTLSSNYEGIDILLNAPADAA"
     gene            1927..2340
                     /locus_tag="PLZ72_14230"
     CDS             1927..2340
                     /locus_tag="PLZ72_14230"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNA37502.1"
                     /translation="MAAALPLQVDIELEQGVSVFGGPGATAVAVVLLLIIAVSLWRVF
                     TRMGQPGWTGAVPIVNFVSIAALAGRPWWIGLLPIVPCIGLVAVVLLCRDLARMFGHG
                     VLFTIGLVLLPFVFLPILAFSPREHYQGRTVRVFG"
     gene            2337..3680
                     /locus_tag="PLZ72_14235"
     CDS             2337..3680
                     /locus_tag="PLZ72_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37503.1"
                     /translation="MTGNRRAGSRTWLRWAGVLAVALIGAGIGASLLGRTTASTAVGD
                     VELTSRPALSGGATLSLAPLGSVDLPSAGPMRLQARLVALDDELLVRAGRGLARGEQP
                     VPASEAARIEDEVASQIVDSVGPLVLRTVAGGLIGAALLTLLVYRARREAIVGAAVGA
                     VSVVALCTVAFTSIPDGVDDAQVNGAIGLLPQADPGLIEGAEGVQDYIGKTFRNIEGL
                     YTGYVRSVAAGRIARTSDRVIAVSGAPSEDAMRRVAVLADSQGAKGVLWIVTDRPGAG
                     SDAGSDAEPGDEPDESSEPDERSGPDAEGGAGDPTALGSGSEVPVVFIGPDTAAGFQF
                     LDRFPVLVLGSDGTLPAGSTSGSLDDAALVVDPKGSEVPSDISRPGVSTGKRPSARGA
                     GLTDASGTVRVTTGTIDDDRLEAVVISYGRNGPDIVQAQLVTVANGDIAVTRVLR"
     gene            3677..5134
                     /locus_tag="PLZ72_14240"
     CDS             3677..5134
                     /locus_tag="PLZ72_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNA37504.1"
                     /translation="MNDDPTPGADGPADPLIPRSALRSAALTTVLALVGATVALWLIG
                     PVRVAVGPVTTGVSVTPALGGPVTLDLAPLGQVEIAPTYAPIRLRAEVADLDPAAVSD
                     VVTKGRAPELDLGSTLPRTLIGAIVTYVGVAALGAGLVAGIGRRSWRHGVAGACIPFL
                     ALSACAWIGLFTLNVDELADTRTTGVLGLVPDGAANAAQFFDDRDRFADNVAANTEAT
                     TDLYAGLFGMADPDHPDVVRILHISDLHLNSAALAFAGRLATTFDVDVVANTGDDADW
                     GSDVESEILGGPVGLDVPYLWVRGNHDTAVTQTANAGEGAVVLDGTETTVDDLVFFGV
                     GDPTFSPKLDKAVVKEGQTAFKQRWSTETMLPLLAAQPQRPDVVMTHDPAMTDAIEPK
                     PPLRLAGHTHKLKVSGGTNPDGTPWVLVVNGSTGGAGLRVLDRKEENPMSAVIITVDR
                     QTKQALYLDEFTYRPLVDQTFKVRRIDLRNLDPAD"
BASE COUNT          745 a         1882 c         1812 g          780 t
ORIGIN      
        1 tcgccatggt cggcgtgctg cgcggcgtcg ccgagcgtgc cccccgactg agccgacccg
       61 ccgcggacgc cttcatcgcc gacgccatca ccgcggtgct gcgcaccacg ccggtccatg
      121 cgatcgaccc gggtcggctc cacccctccg acgccgtgac ctggcgcctg cccccgtcac
      181 cacgcgagcg catcctcgcc gccgccctcc ccctgttccg ctcccgaggg ttcgacgggg
      241 tgggcatcgg cgagatcggc gaccacgccg gagtcggcgc cagcaacgtc tcccgctact
      301 tcggctccaa ggaggaggtt ctggtcgaca tctacgaccg cgtcggagcc agggtggagg
      361 tcgcagtgga tgacgccgtc gcctccgcgt cggacgccga ccacgcgctc cagaaccttc
      421 tcgaggccta caccgaggtc gccttcggtg ccgcggacct ggtggtggtc gtgggcgaga
      481 accgcggagc gctgccgcgg aacgagatcg cccgcctccg gcgtcgggac cgtcgggtcg
      541 ccgacatctg gcggggggtc atcgccgcgc tgcgacccga gctccggccc gtcgagacca
      601 ccacggtcgt cgccggtgtc atcgccctgg tcaacagctt cccccgacac gaacccgacg
      661 ctctgcccgc tccgtcagcg gtcgtgcccc tggcccgcgc cttcgtcggg gccgcgtagg
      721 ccgggccgga cgggccctgt cgggtctcag ctctcgaggg cggcacggac ctgggccagc
      781 cggtcggcga cgatcgtgca gttgacccac cgaccgacct tctccttggt gatcagcccc
      841 gcgtcggcca gcaccgacag gtggtgcgag acggtgggct ggctcttgcc gaccggctcc
      901 tcgagatcgc agctgcacgc cgtggcgttc ggagcggcgg cgatcagcga cagcagctgc
      961 aaccggaccg gatcggcgag cgccttgaac actgcggcca gctcgaccgc gtcggtgggg
     1021 tcgaggggct cggcgagcag cgccggacag cactcggcca cgtcatcgga ccgaacggtg
     1081 aggcgcctgg tgctcgtcgc catggtcgac acctggattc cgatgactcc tggtgccaca
     1141 ggtggcacca ggagtcatgg aaatcgaacc ggacctacgc cgcgtcggcg ggggcgttca
     1201 ggaggatgtc gatgccctcg tagttgctcg acagggtctt cccgtccggt gacaggtagc
     1261 cccgcagcgg aacgttgttg aaggtcatgt cgacgacctc acagctcacg ttgcccgaga
     1321 agctctgcac tccgtagttc agcgaggtga gatcgatagt cccgtagaac aacgagcccg
     1381 acaccgacac gttggccgac aggttcatcg gcccggtcca gattccgggt cgcatgggcg
     1441 ggcacgggac gatcgcgatc ttccgctgca cacgcggcga cgggtcgtgg gtggcgtcac
     1501 cgggttgatc ggcgttgagc acgcagttgc ccagcgagtt gaagctgacg acgccgccca
     1561 cgaacgtgca tccggtgctc gacgcgtcga gagtaatcgt gaccggcatg cccgaggtgt
     1621 cggtcgcggt cggcgtgaag gtacgcccga tgtacgagta cgacggagcg ttgaagacga
     1681 tgccgcggta cggcggcggc tcgggtgcgc acgaggcgag gaggccgatg gcccccagca
     1741 ccagcagcgg tgcaccgaga aggcggacac gtgacgactt cgacatttgg atttccccct
     1801 acggcctggt ccgcccaacg tagcagccgc cggggtgggg gacggggtga aacgtgaaca
     1861 cccctcagtc cccgtcggcc gccgcaccga cgaggtcgcg tgcaccccgc agtagcgtca
     1921 cctccgatgg ccgcggcgct ccccctccag gtcgacatcg agctcgagca gggtgtcagc
     1981 gtcttcggtg gacccggagc gaccgccgtc gccgtcgtgt tgctcctgat catcgcggtg
     2041 tcgttgtggc gggtgttcac caggatgggc caacccggct ggaccggtgc ggtacccatc
     2101 gtgaacttcg tctcgatcgc ggcgcttgcc ggccggccgt ggtggatcgg gctgctgccc
     2161 atcgtcccgt gcatcggact ggtcgctgtc gtccttctgt gccgtgacct ggcccggatg
     2221 ttcggccacg gcgtcctgtt caccatcggc ctggtgctgc tgcccttcgt gttccttccg
     2281 atcctggcgt tctcccctcg ggagcactac cagggtcgga cggtgcgggt gttcggatga
     2341 ccggcaatcg ccgggccggc tcccggacgt ggctgcgctg ggccggcgtg ctggccgtcg
     2401 ctctgatcgg agccggcatc ggagcgtccc tcctcggacg cacgaccgcg agcaccgcgg
     2461 tgggagatgt ggagttgacc agccggccgg ccctgagcgg tggggccacg ctgagcctgg
     2521 ccccgctggg atcggtcgac ctgccgagcg ccgggccgat gcgcctgcag gcacggctgg
     2581 tcgcgttgga cgacgagctg ctggtgcgcg ccggccgtgg actcgcccgc ggtgagcagc
     2641 cggtccccgc gagcgaggcc gctcggatcg aggacgaggt cgcgtcccag atcgtcgact
     2701 ccgtcggtcc cctcgtgctc cggaccgtcg cgggaggcct catcggagcc gctctgctga
     2761 cgctcctggt ctaccgcgct cgacgggagg cgatcgtcgg agccgccgtc ggagcggtga
     2821 gcgtcgtggc gctgtgcacc gtggcgttca cgagcatccc cgacggcgtg gacgatgctc
     2881 aggtcaacgg ggcgatcggc ctgctccccc aggccgatcc gggtctcatc gaaggtgccg
     2941 aaggggtgca ggactacatc ggcaagacct tccgcaacat cgagggcctc tacacgggct
     3001 acgtccggtc cgtcgccgcc gggcggatcg cccggacctc ggaccgggtg atcgctgtga
     3061 gcggcgcccc ctccgaggac gcgatgcgtc gcgtggcggt gctcgccgac agccagggtg
     3121 ccaagggcgt cctgtggatc gtgaccgacc ggcccggcgc aggatccgac gcgggatccg
     3181 atgccgagcc cggcgacgaa cccgacgaga gctccgagcc cgacgagcga tcggggcccg
     3241 acgccgaggg cggtgcaggg gacccgaccg ctctgggctc cggttccgag gtgccagtcg
     3301 tgttcatcgg gccggacacc gctgcgggct tccagttcct ggatcgcttc ccggtgttgg
     3361 tgctgggctc cgacggcacc cttccggccg ggtcgacatc cgggtcgctc gacgatgccg
     3421 cgctcgtcgt cgacccgaaa ggatccgagg tgccctccga catctcgcga cccggcgtga
     3481 gcaccgggaa gcgcccctcg gcccgcgggg ccgggctgac cgacgcgtcc ggaacggtcc
     3541 gggtcacgac cggcaccatc gacgacgatc gcctggaggc ggtggtgatc agctacggcc
     3601 gcaacggccc cgacatcgtc caggcccagc tcgtcaccgt cgcgaacggc gacatcgccg
     3661 tgaccagggt gctgcgatga acgacgaccc gacgcctggt gccgacgggc cggccgaccc
     3721 gttgatcccc cgctccgccc tgcgttccgc ggcgctgacg accgtgctcg cgctcgtcgg
     3781 ggccaccgtc gcgctgtggc tgatcggacc ggtccgggtc gcggtcggac cggtgaccac
     3841 cggcgtgtcg gtgacgcccg cactcggcgg cccggtcacc ctcgacctcg cgccgctcgg
     3901 gcaggtggag atcgcgccca cctacgcccc gatccggctc cgggccgagg tggcggacct
     3961 cgaccccgcc gcggtcagcg acgtcgtcac caaggggcgc gctccggagc tcgacctggg
     4021 atccaccctg ccccggaccc tgatcggagc gatcgtcacc tacgtcggcg tcgccgcgct
     4081 cggcgcgggc ctggtcgccg gcatcggacg ccgcagctgg cgacacgggg tagccggcgc
     4141 ctgcatcccg ttcctggcgc tcagcgcctg tgcctggatc ggcctgttca ccctgaacgt
     4201 cgacgagctg gccgacaccc gcacgaccgg cgtgctcggt ctcgtccccg acggcgccgc
     4261 gaacgccgcg cagttcttcg acgaccggga ccggttcgcc gacaacgtcg ccgccaacac
     4321 cgaggcgacg accgacctgt acgccggcct gttcgggatg gcggaccccg atcaccccga
     4381 cgtggtccgg atcctccaca tcagcgacct gcacctcaac tccgccgcgc tggccttcgc
     4441 cggccggctg gcgacgacct tcgacgtcga cgtcgtcgcc aacaccggcg acgacgccga
     4501 ctggggcagc gacgtcgagt ccgagatcct cggcggcccg gtcggcctcg acgtgccgta
     4561 cctgtgggtg cgcggcaacc acgacacggc cgtcacccag accgccaacg ccggtgaggg
     4621 cgccgtggtg ctcgacggca ccgagacgac cgtcgacgac ctggtgttct tcggggtcgg
     4681 cgaccccacg ttctcaccca agctggacaa ggcggtggtc aaggagggtc agaccgcgtt
     4741 caagcagcgg tggtcgaccg agacgatgct gccgctgctc gcggcccaac ctcagcggcc
     4801 ggatgtggtg atgacccacg acccggcgat gaccgatgcc atcgagccca agccgccgct
     4861 gcgcctggcc ggccacaccc acaagctgaa ggtgtcggga gggaccaacc ccgacggcac
     4921 gccgtgggtg ctcgtcgtga acggatcgac cggaggagcg ggcctgcggg tgctcgaccg
     4981 caaggaggag aacccgatga gcgcggtgat catcaccgtc gaccggcaga ccaagcaggc
     5041 gctctacctc gacgagttca cctatcggcc actggtcgac cagaccttca aggtccggcg
     5101 catcgacctg cggaacctcg accccgccga ctgaccgtcg accccgaccc cccacccccg
     5161 cgtcccgttc tgttcgttgg ttcacaacgc tgacgttgtg aaccaacgaa cagaacggg
//
LOCUS       DAOQOD010000200         5150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6643_length_5150_cov_11.176055, whole genome shotgun sequence.
ACCESSION   DAOQOD010000200 DAOQOD010000000
VERSION     DAOQOD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6643_length_5150_cov_11.176055"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLZ72_14245"
     CDS             complement(<1..481)
                     /locus_tag="PLZ72_14245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNA37505.1"
                     /translation="MEHLRPSNLPSGIRLIEVVGRGGSAVVWRARDRRSGRDVAVKVI
                     GLPPDGPPRERTRDRFERELRALARLRHDHVLAVSAAGTDRDCCWVLTPLADDSLAGR
                     TDRAPITDPDELCELALAVGSALAAAHRLDVVHGDVTPANVLFCDGRPVLADFGIAVL
                     "
     gene            557..1021
                     /locus_tag="PLZ72_14250"
     CDS             557..1021
                     /locus_tag="PLZ72_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNA37506.1"
                     /translation="MADPEGTLTELHSTVAPRLRGAGLRYTRSRRRVVEVLAGTDRPL
                     TIPDILARAGTLAQSSVYRNLNELIDAGVVHRIVAGDEFSHFELAEELTHHHHHLVCT
                     RCGRVEDFVPTDELESSLHSVLRDVSASSGFALEHHRLDLIGTCAECTRLTG"
     gene            complement(1057..1998)
                     /gene="meaB"
                     /locus_tag="PLZ72_14255"
     CDS             complement(1057..1998)
                     /gene="meaB"
                     /locus_tag="PLZ72_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNA37507.1"
                     /translation="MGSRSSSAAELLDAAVGGDRAALARLLSMVERGGDGARAVARAA
                     FPRAGAAHTIGITGAPGSGKSSLTSALCRVVRASGDRLAVLAIDPSSPFSGGAILGDR
                     VRMADHTLDEGVFIRSMATRGHLGGLSVATPEAVRVLDAVGFPWVVIETVGVGQVEVE
                     VAGAADTVVVVVNPGWGDSVQANKAGLMEIADVFVINKADRPGARDTRRDLDNMLALR
                     GGDHPPVIVETVATRDEGLNELWEAIRDHRRRSTDSGERARRREARVDDEMRRVLVAR
                     LIERAGAMGEDTRWASARRAVLDGTVDPWAATDALID"
     gene            complement(2016..2276)
                     /locus_tag="PLZ72_14260"
     CDS             complement(2016..2276)
                     /locus_tag="PLZ72_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNA37508.1"
                     /translation="MQEQVEQVIEIIRPAIQADGGDINLLDVDESSGVVTVELTGACV
                     SCPASTITMKAGVERIMKDRVPGVTAVVQPDTELELGTAVSL"
     gene            complement(2370..3623)
                     /gene="murA"
                     /locus_tag="PLZ72_14265"
     CDS             complement(2370..3623)
                     /gene="murA"
                     /locus_tag="PLZ72_14265"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNA37509.1"
                     /translation="MSRLLVRPGDPLSGEVVISGAKNSVLKLMAATVLAEGRYVLRNV
                     PDISDVTIMGRLLEAMGMSVRRREDAALEIVRGPDVVAEAPYELVEQMRASTAVLGPL
                     LAAVGRARVALPGGDDFGSRPVDMHLASLEALGATFTLSHGMIEGRSEKLIGAEVVLE
                     YPSVGATENLLMAGATARGTTTVRNAAREPEISDLAAFLNRMGARVLGAGSPTIVIEG
                     VDELRAVEHTVIPDRVEVVTFLAAVAVAGGEVTLRGARADHVDLLIEKMGRMGLRISP
                     EADGLWVMSSGRPRPVDVATLPYPGIATDYLPMLVAVMCLADGTSYATENVFAGRFRY
                     VGELARMGADIRVEGHHLVIDGVERLSGAPVRAVDIRAGAAMAIAALAADGETTIHDA
                     GPIDRGYESFVDKLSGLGADITRQD"
     gene            3720..>5150
                     /locus_tag="PLZ72_14270"
     CDS             3720..>5150
                     /locus_tag="PLZ72_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNA37510.1"
                     /translation="MASRVDEWRGTTTTLAERLRPRDDLIVREVPAGSDTDGDTACDT
                     DRPGSASFGAVEGPFLRYERRVEWEPDGSELRITQRIDWRLGIPYWAWLYRPFVHRAL
                     RDGVAGHRPWWLFPDRISVPQARAVSTMALFSLVSGLLYGQLTQVLTFASADIGDGSS
                     GQQAAIFAFVRIGTVLTAFMMFQADRFGRRRIALWSFGLAIATSVATAFVPSLAALSV
                     LQAVSRNLAVAGLLAIDTLCIEELPAGSRAMASGLGAMAYGLGSGVVVVSLPLADVSP
                     WGWRLVFALSLITVPLVRSGARTSTESHRFLRLHPDRSGSASGTGDPPPESRRIHGSR
                     FVLLAALFVLLNLLVAPMSQLQNDYLRSERGFDGFTIAVFTVLTGTPAALGVIVGGRI
                     ADTRGRRWALVPGLVALAVFIALFFNLSGAQMWVSSLVASVLGTLTVAPLGVLAPELF
                     PTARRGGARGALNVLAVSGSVLGLLLA"
BASE COUNT          675 a         1970 c         1758 g          747 t
ORIGIN      
        1 cgagcaccgc gatcccgaag tcggccagca ccgggcgtcc gtcgcagaac agcacgttgg
       61 cgggcgtgac gtcgccgtgg acgacgtcga ggcggtgcgc tgcggccagt gcggatccca
      121 cggccagggc cagctcgcac agctcgtcgg ggtcggtgat cggcgcccgg tccgtgcggc
      181 cggcgagcga gtcgtcggcc aacggggtca ggacccagca gcagtcgcgg tcggtccccg
      241 cagcgctcac cgcgagcacg tggtcgtggc ggagtcgggc cagggctcgc agctcgcgtt
      301 cgaagcggtc ccgggtccgt tccctggggg gtccgtccgg gggcagcccg atcaccttga
      361 ccgccacgtc gcgcccggag cgccggtccc gggcccgcca caccacggcg ctgccgcccc
      421 gcccgacgac ctcgatcagg cggatccccg acggcaggtt gctggggcgg aggtgctcca
      481 tcggctcaag gtaccgttca cattcccacc cgttcccctt cccacccgtt cccattgcgg
      541 ccggtagtct cgacgtgtgg ccgaccccga gggcaccctc accgagctcc actcgaccgt
      601 cgcgccccga cttcggggag ccggactgcg ctacacccgg tcccggcgac gggtcgtcga
      661 ggtcctggcc ggaacggacc gccccctcac catccccgac atcctcgccc gcgccggcac
      721 gctggcccag agcagcgtgt accgcaacct caacgagctg atcgacgccg gcgtcgtgca
      781 ccggatcgtg gccggcgacg agttcagcca cttcgagttg gccgaggagc tcacccacca
      841 ccatcaccac ctggtgtgca cccgctgcgg ccgggtcgag gacttcgtcc ccaccgacga
      901 gctcgagtcg agcctgcact cggtgctgcg cgacgtgtcg gcctcgagcg gcttcgccct
      961 cgagcaccac cggctcgacc tgatcggcac ctgcgccgag tgcacccggc tgaccggctg
     1021 aacgactgac ggactgaccg gctgctcggg cgtgggtcag tcgatcaacg cgtcggtcgc
     1081 ggcccacgga tcgacggtcc cgtcgagaac cgcccgacgc gccgatgccc accgcgtgtc
     1141 ctcacccatc gcccccgccc gttcgatcag gcgggcgacc agcacccgcc gcatctcgtc
     1201 gtcgacccgg gcctcccggc gccgggcccg ttcgccgctg tccgtgctgc ggcgacggtg
     1261 atcccggatc gcctcccaca gctcgttcag gccctcgtcg cgggtggcca ccgtctcgac
     1321 gatgaccggc ggatggtcgc cgccgcgcag agccagcatg ttgtcgaggt cacgccgggt
     1381 gtcacgcgcc ccgggacgat ccgccttgtt gatcacgaac acgtccgcga tctccatcag
     1441 tccggccttg ttggcctgca ccgaatcacc ccagcccggg ttcaccacga cgacgacggt
     1501 gtcggccgct ccggcgacct cgacctcgac ctggccgacc ccgaccgtct cgatcacgac
     1561 ccacgggaag ccgacggcgt cgagaacgcg caccgcctcg ggggtagcca ccgacaaccc
     1621 tccgaggtga ccgcgggtcg ccatcgagcg gatgaacacc ccctcgtcga gggtgtggtc
     1681 ggccatgcgg acccggtccc ccaggatcgc cccgcccgag aacggcgacg acgggtcgat
     1741 cgccagcacg gccaggcggt cacccgaggc ccgaaccacg cggcacagcg cgctggtcag
     1801 ggacgacttg ccggatccgg gcgccccggt gataccgatc gtgtgggcgg cacccgctcg
     1861 ggggaacgcg gcccgggcca ccgcccgggc gccgtcaccg ccccgctcca ccatcgacag
     1921 cagccgcgcc agcgcggcgc ggtcccctcc gacggccgcg tcgagcagct cggcggccga
     1981 cgacgaacgg gatcccacct cgacgtgccg accgctcaga gcgacacggc ggtgccgagc
     2041 tcgagctcgg tgtcgggctg gacgaccgcg gtcacgccgg gcacacggtc cttcatgatg
     2101 cgctcgacgc cggccttcat cgtgatggtc gaggccgggc agctcacgca ggcgccggtc
     2161 agctcgacgg tgaccactcc ggagctctcg tcgacgtcga gcaggttgat gtcaccgccg
     2221 tctgcctgga tggcgggacg gatgatctcg atgacctgct cgacctgctc ctgcacgggt
     2281 ggcgctccgg ggtgtcggga tcctggggaa cgggaccaga gtagatggcg gcccggcacc
     2341 ctccccaaca ggggtggtgg gccggcccct cagtcctgac gggtgatgtc ggcgcccagt
     2401 cccgacagct tgtccacgaa gctctcgtag ccgcggtcga tcggccccgc gtcgtggatc
     2461 gtcgtctcgc cgtccgccgc gagcgcggcg atggccatcg ccgcaccggc gcggatgtcg
     2521 acggctcgca ccggggcgcc cgacaggcgc tcgacgccgt cgatcaccag gtggtgtccc
     2581 tcgacgcgga tgtcggcgcc catgcgggcc agctcgccga cgtagcggaa ccgcccggcg
     2641 aacacgttct cggtcgcgta gctggtcccg tcggccaggc acatcaccgc caccagcatg
     2701 ggcaggtagt cggtggcgat gcccgggtac ggcagcgtcg ccacgtcgac cggccggggt
     2761 cggcccgacg acatcaccca caacccgtcg gcctcggggg agatccgcag acccatccgg
     2821 cccatcttct cgatcagcag gtcgacgtgg tccgcccggg cgccgcgcag cgtcacctct
     2881 cccccggcga ccgccaccgc ggccaggaag gtgacgacct cgacccggtc ggggatgacg
     2941 gtgtgctcga cggctcgcag ctcgtcgacg ccctcgatga cgatcgtggg gctgcccgct
     3001 ccgaggaccc gcgcccccat gcggttgagg aacgcggcca ggtccgagat ctccggctca
     3061 cgggccgcgt tgcgcaccgt ggtcgtgccg cgggcggtgg cgccggccat cagcaggttc
     3121 tcggtggcgc ccacgctcgg gtactcgagc acgacctcgg cgccgatcag cttctcggag
     3181 cggccctcga tcatcccgtg gctcagcgtg aaggtggcac cgagcgcctc gagtgacgcc
     3241 aggtgcatgt cgacgggccg ggatccgaag tcgtcgccgc cgggcagggc gaccctggcc
     3301 cgtccgaccg cggccagcag cggtccgagc accgcggtcg acgcccgcat ctgctccacc
     3361 agctcgtagg gggcctcggc gaccacgtcg ggaccccgga cgatctccag cgcggcgtcc
     3421 tcacggcggc gcaccgacat gcccatcgcc tcgagcaggc gtcccatgat cgtgacgtcg
     3481 ctgatgtcgg ggacgttgcg cagcacgtag cggccctcgg ccagcaccgt cgcggccatc
     3541 agcttgagca ccgagttctt ggccccggag atgacgacct caccggacag gggatctccg
     3601 ggacggacca gcagtcggct cacgccccca ctctcgcacg accggtgctg tcccgcccga
     3661 tgccagcccg cccgatgcca gcccgcccga tagcatcgtg cgacgtccca ggggggtcgg
     3721 tggcgagcag ggtcgacgag tggcgaggga cgacgacgac gctggccgaa cggctgcgac
     3781 cccgcgacga cctgatcgtc cgcgaggttc ccgccggctc tgacaccgac ggcgacaccg
     3841 cctgcgacac cgaccggccc ggatcggcgt ccttcggagc cgtcgaggga cccttcctgc
     3901 gctacgaacg gcgggtcgag tgggaacccg acggctccga gctgcggatc acccagagaa
     3961 tcgactggcg tctcggcatc ccctactggg cctggctcta ccgcccgttc gtgcaccggg
     4021 cgctgcgcga cggcgtggcc gggcaccggc cctggtggct gttcccggac cgcatctcgg
     4081 tgccgcaggc ccgggccgtg tcgaccatgg cgctgttcag cctggtgtcc ggactgctct
     4141 acggccagct cacgcaggtg ctcacgttcg ccagcgccga catcggcgac ggctcctcgg
     4201 gccagcaggc ggccatcttc gccttcgtcc gcatcggcac ggtgctgacc gcgttcatga
     4261 tgttccaggc cgaccgcttc ggccgccgac ggatcgccct gtggagcttc ggcctcgcca
     4321 tcgccacctc ggtcgccacc gcgttcgtcc cgtcgctggc cgcgctgagc gtgctgcagg
     4381 ccgtgtcgcg caaccttgcc gtcgccggtc tgctcgccat cgacacgctc tgcatcgagg
     4441 agctccccgc gggatcccgg gccatggcgt cgggcctggg cgcgatggcc tacgggctcg
     4501 gatccggggt cgtggtggtg tcgctgccgc tcgcggacgt gagcccctgg ggctggcgcc
     4561 tggtgttcgc cctgtcgctg atcaccgtcc cgttggtgcg cagcggggcc cggacctcga
     4621 ccgagagcca ccggttcctg cgtctgcacc ccgaccggtc ggggtccgcg tccgggaccg
     4681 gcgacccacc tccggagtca cggcgcatcc acggcagccg gttcgtgctg ctcgccgcgc
     4741 tgttcgtcct gctcaacctg ctggtggcgc cgatgtcaca gctgcagaac gactacctgc
     4801 gctccgaacg gggcttcgac gggttcacca tcgccgtgtt caccgtgctg accgggactc
     4861 ccgccgcgct cggggtgatc gtcggcggcc gcatcgcgga cacccgcggc cgacgatggg
     4921 cgctggttcc gggcctggtc gctctcgcgg tgttcatcgc cctgttcttc aacctgtcgg
     4981 gcgcgcagat gtgggtcagc tcgctcgtcg cctcggtgct gggcacgctc acggtcgcac
     5041 ccctgggcgt gctcgcgccc gagctgttcc cgaccgcccg ccgcggcggc gcccgcgggg
     5101 cgctcaacgt cctggccgtg tcgggcagcg tgctcggcct tctgctcgcc
//
LOCUS       DAOQOD010000201         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6656_length_5144_cov_9.568481, whole genome shotgun sequence.
ACCESSION   DAOQOD010000201 DAOQOD010000000
VERSION     DAOQOD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6656_length_5144_cov_9.568481"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /locus_tag="PLZ72_14275"
     CDS             complement(<1..2098)
                     /locus_tag="PLZ72_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA37511.1"
                     /translation="MPADATDDRSAPSAPSAPSAPSAPIGLDIGAALAPDGARTDERV
                     RLDPDDLTTHGVIVGMTGSGKTGLAIALMEEVLASGVPVLAIDPKGDLTNLLLTFPEL
                     RPDDFAPWVPDGSDPAEVATTWREGLADWGIGPERIAALRAGVRMGVYSPGSTSATPL
                     DVVGSLAAPTGGASADPEGVEAEVQAVTSGLLGLVGIDGDPLASPEHLLVSNLVHAAW
                     SAGESLDLPMLLQRIQTPPMRRLGVMDVDTVIAPDARTALAVRLNAILATPGFQGFFG
                     GAPLDPQRLLFGDDGRPSLAVVTVAHLSDAERQLVISRVLASVIRWFRTQPGTDRLRA
                     LVYIDEVAGYAPPTAVPPTKAPILTILKQARAFGVGMVLATQNPVDLDYKAMSNAGTW
                     LVGRLQTERDKARLLEGMAPAAGGVDVSALDSTITSLERRQFVLHRTGGHTPLRFGSR
                     WAMSYLRGPVTGAQLALLPGREEIPVAGPTPAPPPGVDGSADADGGSGSPVMPKVADG
                     VAVRYLDPAAPWAAAVGASPTGTAVQAGVALRVSLLFDDAKASIREVQEWEAVLTPLG
                     EVVDPAAAVTVDHDDRDFRTDAPPDVRYLPTEARIDTKSFYTDLSKRLKDMLIRDKTL
                     TIPVNRTLKLWGRVGETADEFAARCDESAQAAADAEADKIRARLGTRIDRLRGAVDTA
                     QRRADTAARAAESAGRT"
     gene            2165..2614
                     /locus_tag="PLZ72_14280"
     CDS             2165..2614
                     /locus_tag="PLZ72_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37512.1"
                     /translation="MARRKPRSRRNRGGLLGWFTDHPGVLAVAVVAALLVVGVGVAKV
                     MADRAATSATEDRAVEIRRLLDEATPEDFLAFNAGVQREGSIARTVRDQPDFVNVRAT
                     ADLSFIRFQPEGWWSGFTERCIVARVRPDGVTVAVPKSACVRVEAPR"
     gene            2614..3402
                     /locus_tag="PLZ72_14285"
     CDS             2614..3402
                     /locus_tag="PLZ72_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37513.1"
                     /translation="MEHTTEPVPGWPALATRGLGKDFGKRHAVESIDLDVPVGSFFGL
                     VGPNGSGKTTTLRMASGLQRPDRGQVWIAGHDTWAEPVAVRRLLGVVPDPLHLFDRLT
                     ARELLGHLGELRGMDRDEVRRRSDELLAVLGLTEASTELVGSYSHGMRKKTSLAAALV
                     HRPAVLLLDEPFEGVDPVSAVTIRGLLDRYRAAGGTVVFSSHVMDLVERFCDHVAVMS
                     LGRLLAAGPIDSVRSGRRLEDAFIEMVGASPVGSDELAWLDRPR"
     gene            3399..5021
                     /locus_tag="PLZ72_14290"
     CDS             3399..5021
                     /locus_tag="PLZ72_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37514.1"
                     /translation="MTAAGEVTLGRACRSLMSLKWNLLLNGLRGRMQLRVQTIVSLLA
                     SVALGLFGLAAFAGIGQSIAHGDLVVVIALPVIVAGIALLSAAAGVETTIDLRYLAAE
                     PIGAHRLGSAALAAAVVGPPALLAGLSGVGLLLGFAPGSAVAGWVVAAATVLAWWATL
                     LLASRTAANGLGVLAHGRSRHVAQTLAAFAAVGMWFAAQLGLQAIQTWDRSRWERLAE
                     VFAWTPPGQLGRALATASSDPAGAAVHLVLGVVWLPLLWWLHGVTTGKLVTAPVRPGT
                     EGRRVRTGTVGVRAGVLALLPPGRATAVGARTVRTKMRTPREAVNTVVALLLGMGALV
                     VAPMLGGSPDGRIVLTAGLLHFAVLFESSNAFGFDGPALWMEVAAGADGPVLVRGKAV
                     ASIAVMAPFAGLLVLALAAFHGGWQWAPAALLLAMGSVLLASGASVMSATLAPFAVPE
                     SPNPFAAGDAGQGCLASLVLLADMVVLGVVSAPVAVAVWWASTQSPALTAAVALSSPL
                     IGAGVLFGCLKLAGSLIEGREAELVARVTPAR"
BASE COUNT          599 a         1771 c         1991 g          783 t
ORIGIN      
        1 ccgtgcgccc ggccgactcc gcggcccgtg ctgcggtgtc agcccggcgt tgggcggtgt
       61 cgacggcgcc gcggagccgg tcgatccggg ttcccagccg ggcccggatc ttgtcggcct
      121 cggcgtccgc ggcggcctgg gcggactcgt cgcagcgcgc cgcgaactcg tccgcggtct
      181 cgccgacccg cccccagagc ttcagcgtgc ggttgaccgg gatggtgagg gtcttgtcgc
      241 ggatcagcat gtccttcagg cgcttggaca gatcggtgta gaagctcttg gtgtcgatgc
      301 gggcctcggt gggcaggtag cgcacatcgg ggggagcgtc ggtccgaaag tcgcggtcgt
      361 cgtggtcgac ggtcaccgcc gcggccgggt cgaccacctc gccgagcggg gtcagcaccg
      421 cctcccactc ttggacctcg cggatcgagg ccttggcgtc gtcgaacagc agcgagaccc
      481 ggagcgcgac cccggcctgc accgcggtgc cggtcggcga cgcaccgacg gcggccgccc
      541 acggcgcggc cggatccagg tagcggaccg ccacgccgtc ggcgaccttc ggcatgaccg
      601 gcgatcctga gcccccgtcg gcgtcggcgg agccgtcgac tccgggtgga ggcgcggggg
      661 tcggtccggc gaccgggatc tcctcgcgtc cgggcagcag cgccagctgc gcgccggtca
      721 ccggaccccg caggtacgac atcgcccaac gcgacccgaa ccgcagcggg gtgtggcctc
      781 cggtccggtg caacacgaac tgtcgccgct cgagcgaggt gatcgtcgag tcgagggcgg
      841 agacgtcgac gccgccggcg gcgggggcca tcccctcgag caggcgggcc ttgtcacgct
      901 cggtctgcag gcgcccgacc aaccacgtcc cggcgttgga catcgccttg tagtcgaggt
      961 cgaccggatt ctgcgtggcc agcaccatcc cgaccccgaa cgcccgggcc tgcttgagga
     1021 tggtcaggat cggtgccttg gtgggcggca cggcggtcgg cggggcgtag cccgcgacct
     1081 cgtcgatgta gacgagcgcc cgcaggcggt cggtgccggg ctgggtgcgg aaccaccgga
     1141 tgaccgacgc cagcaccctg ctgatcacca gttggcgttc ggcgtcggag aggtgggcga
     1201 cggtcaccac cgcgagcgac ggtctcccgt cgtcgccgaa cagcaggcgc tgcgggtcga
     1261 gcggggcgcc gccgaagaag ccctggaacc ccggcgtggc caggatcgcg ttgagccgca
     1321 ccgccagagc agtgcgggcg tcgggggcga tcaccgtgtc gacgtccatc accccgaggc
     1381 ggcgcatcgg cggggtctgg atgcgctgca gcagcatggg gaggtcgagc gactcacccg
     1441 cgctccacgc cgcgtgcacc aggttcgaca ccaggaggtg ctccgggctg gcgagcggat
     1501 cgccgtcgat gccgaccagg ccgagcaggc ccgaggtcac cgcctgcacc tcggcctcga
     1561 cgccctccgg atcggccgac gctccgccgg tgggcgccgc cagcgacccg acgacgtcga
     1621 gcggcgtggc cgacgtcgac ccggggctgt agacacccat gcggaccccg gcccgcagcg
     1681 ccgcgatccg ctccggaccg attccccagt cggccaggcc ctcgcgccac gtggtcgcga
     1741 cctcggccgg gtcggagccg tcggggaccc agggcgcgaa gtcgtcgggt cgtagctcgg
     1801 ggaacgtcag cagcaggttg gtcaggtcgc ccttggggtc gatggcgagc accgggacgc
     1861 cggaggccag cacctcctcc atcagggcga tggccagacc ggtcttgccc gacccggtca
     1921 tgccgacgat caccccgtgg gtggtcaggt cgtcgggatc gaggcggacc cgctcgtcgg
     1981 tgcgggcgcc gtcgggcgcg agcgccgcac cgatgtccag gccgatcggc gcagacggtg
     2041 cagacggtgc agacggtgca gacggtgcag agcggtcatc ggtggcgtcg gccggcatgg
     2101 acagagcctg tcacccgcgg gccggtcacc gccgcgctcg ggtgggcgtg cgatcattcc
     2161 gacgatggcg cggcggaaac cacgatcccg acggaaccgc ggcggcctgc tcggctggtt
     2221 caccgatcac cccggcgtgc tggccgtcgc cgtggtcgcg gcgctgctgg tggtcggggt
     2281 cggggtggcc aaggtgatgg cggaccgggc ggcgacgtcg gccaccgagg accgggcggt
     2341 cgagatccgt cggttgctcg acgaggccac cccggaagac ttcctggcgt tcaacgcggg
     2401 ggtgcagcgt gagggcagca tcgcccgcac ggtccgggac cagcccgact tcgtcaacgt
     2461 gcgggcgacc gcggacctca gcttcatccg tttccagccc gaggggtggt ggtcggggtt
     2521 caccgagcgc tgcatcgtgg cccgggtccg ccccgacggc gtgaccgtcg cggtccccaa
     2581 gtcggcgtgc gtgcgcgtcg aggccccccg ctgatggagc acacgaccga accggtgccg
     2641 ggctggccgg cgttggcgac ccgggggctc ggcaaggact tcgggaaacg acacgcggtc
     2701 gagtcgatcg acctcgacgt cccggtcggg agcttcttcg gcctggtcgg gcccaacggc
     2761 tcgggcaaga ccaccactct gcgcatggcg tcgggtctgc agcggcccga tcggggtcag
     2821 gtctggatcg cgggccacga cacctgggcc gagccggtgg cggtccgccg gcttctcggc
     2881 gtcgtgcccg atccgctcca cctgttcgat cggctgaccg ccagggagct gctcggccac
     2941 ctgggcgagc tgcgcggcat ggaccgcgac gaggtgaggc ggcgcagcga cgagctgctg
     3001 gcggtgctgg ggctcactga ggcgtcgacc gagctggtcg gcagctacag ccacggcatg
     3061 cgcaagaaga cgtcgctggc cgccgcgctt gtgcaccgac cggccgtgct gctgctcgac
     3121 gaaccgttcg agggcgtcga cccggtgtcc gcggtcacca tccggggact gctcgaccgc
     3181 taccgagccg cgggcgggac cgtggtgttc tcgagccacg tcatggatct ggtcgagcgg
     3241 ttctgcgacc acgtagcggt gatgtcgctc ggtcggctcc tggccgcggg tccgatcgat
     3301 tcggtccggt ccggccgtcg gctcgaggac gcgttcatcg agatggtcgg ggcctcacct
     3361 gtcggcagcg acgagctggc ctggctggac cgtccgaggt gaccgcagcg ggggaggtga
     3421 cgctcggccg ggcgtgtcgg agcctgatgt cgctcaagtg gaacctgctg ctcaacggcc
     3481 tgcgcgggcg gatgcagctg cgggtccaga ccatcgtgtc gctgctggcg tcggtggcgc
     3541 tcggtctgtt cggtctggcc gccttcgcgg gcatcggaca gtcgatcgcc cacggggacc
     3601 tggtcgtcgt catcgcgctg ccggtgatcg tggccgggat cgccctgctg tccgccgcgg
     3661 caggggtgga gacgacgatc gacctccgct acctggccgc cgagccgatc ggtgcacacc
     3721 ggctgggatc ggccgcgctc gcggctgctg tggtcgggcc gccggcgctc ctggccggcc
     3781 tgtcgggcgt cggcctgctc ctgggcttcg ctccggggtc agccgtggcg ggatgggtgg
     3841 tggccgccgc cacggtgctg gcgtggtggg cgacgctcct gctcgcgagt cgcactgcgg
     3901 ccaacgggct cggggtgctg gcccacgggc ggtcccgcca cgtcgcgcag acgctggctg
     3961 cgttcgcggc ggtcgggatg tggttcgccg cgcagctcgg actgcaggcc atccagacct
     4021 gggatcggtc ccgttgggag cgtctggccg aggtgttcgc ctggacccct ccgggtcagc
     4081 tcggtcgggc gttggccacg gcgtcgtcgg acccggccgg tgctgctgtg cacctggtgc
     4141 tcggagtggt gtggttgccg ctcttgtggt ggctgcacgg ggtgacgacc gggaagctcg
     4201 tgaccgcccc ggtgcgtccg gggaccgagg gccgacgggt gcgaaccggc accgtcgggg
     4261 ttcgcgccgg tgtgttggcg ttgctgcctc ccggacgggc caccgcggtc ggcgcccgca
     4321 cggtgcggac caagatgcgc acaccccggg aggcggtgaa caccgtcgtc gcgctgctgc
     4381 tggggatggg ggcgctggtc gtcgccccga tgctcggcgg atcccccgac ggtcggatcg
     4441 tgctgaccgc cggcctgctg cacttcgcgg tgctgttcga gtcctccaac gcgttcggct
     4501 tcgacggccc cgcgctgtgg atggaggtgg cggcgggggc cgacgggccg gtgctggtcc
     4561 ggggcaaggc ggtcgcctcg atcgcggtga tggcgccgtt cgccgggttg ctggtgctgg
     4621 ccctggctgc gttccacggc gggtggcagt gggccccggc ggctctgctg ctggcgatgg
     4681 ggtcggtgct gctcgccagc ggggcgtcgg tgatgtcggc gacgttggcg ccgttcgcgg
     4741 tgcccgagag ccccaacccg ttcgcggccg gtgacgccgg tcagggctgc ctggccagcc
     4801 tggtgctgct cgccgacatg gtcgttctcg gcgtggtgtc ggccccggtg gccgtcgcgg
     4861 tgtggtgggc ctcgacgcag tcgcccgcgc tgaccgctgc ggtcgccctg agcagccccc
     4921 tcatcggcgc cggggtcctg ttcgggtgcc tgaagctggc cgggtcgctg atcgagggcc
     4981 gtgaggccga gctcgtcgcc cgtgtcaccc cggcgcgctg acgggccggc ccgctcagca
     5041 tcccccaccc ctgcccgttc tgttcgttgg atcacaacgc gggagttgtg atccaacgaa
     5101 cagaacgggg gtggggcgcc ccacccccgt tctgttcgtt ggat
//
LOCUS       DAOQOD010000202         5129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6685_length_5129_cov_11.277296, whole genome shotgun sequence.
ACCESSION   DAOQOD010000202 DAOQOD010000000
VERSION     DAOQOD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_5129_cov_11.277296"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLZ72_14295"
     CDS             complement(<1..1046)
                     /locus_tag="PLZ72_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNA37515.1"
                     /translation="MAPTPYDPSRPYWVQGNFAPVAAEETVTELAVTGEIPAELTGLF
                     VRNGSNPATGEALHWFLGDGMVHGVRLDGGKALWYRNRYVTTPLQQSGKGLMEFGGVP
                     GQQNNQSNVAVVEHGGRFLSLGEVGWPYEMSTEDLSTVGPFDYAGKLGPTMTAHPKVD
                     PETGRMHFFGYEFMRPALTYYAADSDGTLDVVSPITVPATTMIHDFAVTDRDAVFWIG
                     PVVFGPDPTGTSQIPFHWDPTGPSRIGVMPLDGSGADIRWVDVPPSFAFHGYNAHRDG
                     DDVVLRVHRADEAFGPKGDLVSTLLTEWRIGTAGPSLTFAEEQISDRPMDLPSHDRRR
                     TGRETRHGWFVTTTS"
     gene            1327..1941
                     /locus_tag="PLZ72_14300"
     CDS             1327..1941
                     /locus_tag="PLZ72_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNA37516.1"
                     /translation="MSSGLTPRPSSGDPDDDLMVDVARTVTGAWWIYLIAGIAWFVLA
                     FVVLTVDAASVWTVAILAAIAFIVGGIFELVLASMVPSWKWVHVLFGVISIIAGIVAL
                     AWPQQTFVVLAAVLAWYLLIMGIVEIVVAFMTRHEDGMWWLRLVIGIVEVLVGFWAVA
                     YIGRAIVLLVVWVAAAALARGLSDLFIAFGLHSAHSHLDAERSS"
     gene            1995..2615
                     /locus_tag="PLZ72_14305"
     CDS             1995..2615
                     /locus_tag="PLZ72_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNA37517.1"
                     /translation="MATLAVAVALIPAAACSGDDDGSASTSDAGTTTGGTEAAAIRFD
                     ADAQRELARVGCHPGTVDGVMGPKTDEAVRNFQAASGLDVDGELGPETAKALAEAADA
                     DETVCSTSTTATPTTATPTSAGSATAPSGPTPCTASALMGGLPSQSGTISRFVCSDGY
                     AAGTLSDGTRFLLRSQDGRWYAPGQDPCGSASAGIPPVVLADGCPD"
     gene            complement(2653..3459)
                     /locus_tag="PLZ72_14310"
     CDS             complement(2653..3459)
                     /locus_tag="PLZ72_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNA37518.1"
                     /translation="MAGPGVDRAERSVDGVLLDIEGVLMSSSAPLPGAARTVDALRRR
                     GLPIRFVTNTTSTPAARIAAGLIHAGIEVRAGELVTAASVTAAYLREHHPDARCLVLN
                     DGPNADLAGVRLADPDDPTADVVVVGGGGPSFTWKQMNTALNCLLNGADLVAMHGAPV
                     WHTPEGFCLDGAAYARMLESASGVDAVVAGKPAPHMFLAAAESTGAPPERLIMVGDDL
                     ESDVLAAQALGMVGVQVRTGKFRQQTLDRSPKAPDHVIDSIADLLGLIDA"
     gene            complement(3547..4629)
                     /locus_tag="PLZ72_14315"
     CDS             complement(3547..4629)
                     /locus_tag="PLZ72_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37519.1"
                     /translation="MSAAVLLDLGALDPALRLDGHLPECPHRRRPPDGCDWCLWAVEG
                     AGVRDALDVIRDADTLTRDHDRRGGDVSVAWVVATGDLLDACSGEQLGASSPVPSVRE
                     LADRLGRLRDDRCRPLRRRLDSATAADGPVERRCLSTAGPLAAAALQRPEHTELRDRL
                     SAPVRDRLDHATSLLSADTQTAGLLPSVEARHWDGLPALRTQTAWSRRRRPDPDRSRT
                     GWSGSDRWDADRQEPGRTGPHEPARPAPGSLEALVVESVIDGVVDELAELGVDLAAAS
                     DPVAVPRPPSRTRMSERTRAMSWRIARIDWHLTYLDTGRAGGWPATTSVTPDGTGSVA
                     DLVPWTVALAIAEGERAGLVSATHTC"
     gene            complement(4626..>5129)
                     /locus_tag="PLZ72_14320"
     CDS             complement(4626..>5129)
                     /locus_tag="PLZ72_14320"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNA37520.1"
                     /translation="RATTRHTEPDWSRPDRRAASTPGVLRPGQRRLRSSVAVVIDTSA
                     SMNSPRLDAAVTELDALLHHGGVAPVPVVVCDELAARTQTVRRLSELCLSGGRGTDLR
                     VGIAAAAALRPLPRLIVVLTDGWTPWPDTAPPRTSVLAVIIDESEHRVAPAPGGDGFR
                     TVRVDTP"
BASE COUNT          657 a         1830 c         1918 g          724 t
ORIGIN      
        1 gaggtcgtgg tcacgaacca gccgtggcgg gtctcgcggc cggtgcgtcg acggtcgtgg
       61 ctgggcaggt ccatcggccg gtcgctgatc tgctcctcgg cgaacgtgag cgaggggccg
      121 gcggtgccga tgcgccactc ggtcaacagc gtgctgacca gatcgccctt gggcccgaag
      181 gcctcgtccg cgcggtgcac ccgcagcacc acgtcgtcgc cgtcgcggtg cgcgttgtag
      241 ccgtggaacg cgaacgacgg cggcacgtcg acccagcgga tgtcggcccc ggacccgtcg
      301 aggggcatca cgccgatccg gctcggtccg gtcggatccc agtggaacgg gatctgcgag
      361 gtcccggtgg ggtcggggcc gaacaccacc ggaccgatcc agaacacggc gtcgcggtcg
      421 gtgacggcga agtcgtggat catcgtggtg gccggaaccg tgatcgggct gacgacgtcg
      481 agcgtgccgt cggagtccgc cgcgtagtag gtgagggcgg gccgcatgaa ctcgtagccg
      541 aagaagtgca tccggccggt ctcggggtcg accttggggt gtgcggtcat ggtcgggccg
      601 agcttgccgg cgtagtcgaa cgggccgacc gtcgataggt cctcggtcga catctcgtag
      661 ggccagccga cctcgcccag gctgaggaac cggccgccgt gctccaccac cgccacgttg
      721 ctctggttgt tctgctggcc ggggaccccg ccgaactcca tgaggccctt gccggactgc
      781 tgcagcggcg tggtcacgta gcgattgcgg taccagaggg ccttgccgcc atcgaggcgg
      841 acgccgtgga ccatcccgtc gccgaggaac cagtgcagcg cctcgccggt ggcggggttc
      901 gagccgtttc gcacgaacag gccggtgagc tcggcgggga tctcaccggt gacggccagt
      961 tcggtgacgg tctcctcggc ggcgacgggg gcgaagttgc cctgcaccca gtacggccgg
     1021 gacgggtcgt agggggtggg tgccaccgtc ggagtcggcg ctgtggtcga cgtggcgccc
     1081 gctcctccgg ctgctgctcc agctgctctt ccgggagctc ctgaggcgtc gtcggaacct
     1141 ccaccgcagg cggacagagc gcccgcgccg atcgcggcgc cgcccaggcc cagagccgtc
     1201 agcagcttcc gtcggtgcat cggtccctcc ggggtcgtga cggccacagg gcgtggcggt
     1261 cgcagccgcc catggtggca cgggggaacc gccgaacgcg accggcgggg tcctagggtg
     1321 gccgccatga gcagcggact gaccccccgg ccctcctccg gtgaccccga cgacgacctc
     1381 atggtcgacg tggcccggac cgtcaccggt gcctggtgga tctacctcat cgcgggcatc
     1441 gcctggttcg tcctggcctt cgtcgtgctg accgtcgacg cagccagcgt gtggaccgtg
     1501 gcgatcctgg ccgcgatcgc gttcatcgtc ggcggcatct tcgagctggt gctggcgtcg
     1561 atggtgccgt cgtggaaatg ggtccacgtc ctgttcggcg tcatcagcat catcgccggc
     1621 atcgtcgcac tggcgtggcc gcagcagacc ttcgtcgtcc tggccgcggt cctggcctgg
     1681 tacctgctga tcatgggcat cgtcgagatc gtcgtcgcgt tcatgacccg tcacgaggac
     1741 ggcatgtggt ggctgcgcct ggtcatcggc atcgtcgagg tcctcgtcgg cttctgggcg
     1801 gtggcctaca tcggtcgggc gatcgtcctg ctcgtggtct gggtggccgc cgccgcgttg
     1861 gcccgcgggc tgtcagacct gttcatcgcc ttcgggctgc acagcgccca cagccacctg
     1921 gacgccgagc gttcctcgtg accgctcgtc ggtcgcgtcg cgggccgcgg ctccgttgct
     1981 ccggggcgcg ggtcatggcc acgcttgcgg tcgcggtcgc tctgatccca gccgcggcct
     2041 gcagcggtga cgacgacggc tcggcctcga cgtcagatgc gggcaccacg accggcggga
     2101 cggaggcggc agcgatccgc ttcgacgccg acgcgcagcg ggagctcgcc cgcgtggggt
     2161 gtcacccagg gacggtcgac ggtgtgatgg gccccaagac cgacgaagcg gttcggaact
     2221 tccaggccgc gtcgggcctg gacgtcgacg gcgagctcgg acccgagacc gcgaaagccc
     2281 tggccgaggc cgccgacgct gacgagaccg tctgctccac ctcgacgaca gccaccccga
     2341 cgacagccac cccgaccagc gccggatcgg ccacagctcc gagcggtccg acgccctgca
     2401 ccgcgtcggc gctgatgggt ggtctcccgt cgcagagcgg gaccatctcc cggttcgtgt
     2461 gctccgacgg ctacgcggcc ggcaccctga gcgacggcac ccgcttcctg ctgcggtccc
     2521 aggacggccg ctggtacgcc cctggccagg acccgtgcgg atccgccagc gccgggatcc
     2581 cgccggtcgt gctggccgac ggctgccctg actgatcccc ggccctgacc ggcccccggc
     2641 cctgaccggc cctcaggcgt cgatcaggcc gagcagatcg gcgatcgagt cgatgacgtg
     2701 gtcgggcgcc ttcggcgacc ggtccagggt ctgctggcgg aacttgccgg tgcggacctg
     2761 gaccccgacc atgcccaagg cctgagcggc gagcacgtcg ctctcgaggt cgtcgcccac
     2821 catgatcaac cgctccggcg gtgctccggt cgactccgcc gcggcgagga acatgtgggg
     2881 cgccggcttg ccggccacca ccgcgtcgac cccgctggcg gactcgagca tgcgggcgta
     2941 cgcggctccg tcgaggcaga acccctcggg ggtgtgccag accggggccc cgtgcatggc
     3001 caccaggtcc gcgccgttca gcaggcagtt gagcgctgtg ttcatctgct tccaggtgaa
     3061 cgacggaccg ccaccgccga cgaccaccac gtccgcggtc ggatcgtcgg gatcggccaa
     3121 cctcacgccg gccagatccg cgttggggcc gtcgttgagg accaggcagc gtgcgtcggg
     3181 atggtgctcc cgcaggtacg cggcggtgac cgacgccgcg gtgaccagct ctccggcgcg
     3241 gacctcgatc ccggcgtgga tgaggccggc ggcgatgcgc gccgccgggg tggaggtcgt
     3301 gttggtcacg aagcggatcg gcagcccccg tcggcgcagg gcgtcgaccg tccgggcggc
     3361 gccgggcagc ggagcggacg acgacatcag gacgccctcg atgtcgagga gcaccccatc
     3421 gacggatcgc tccgcccgat cgacgccggg tccggccacg tcacctccgt tcggcccgat
     3481 cctacgaccg accggggtgt ccgaaccggc gggcgtgccg gcggtcggga cgacgtcgcg
     3541 gtccgctcag caggtgtggg tcgcgctgac gagcccggcc cgctcgccct cggcgatggc
     3601 gagcgccacg gtccagggga cgaggtctgc gacggatccg gtcccgtcgg gggtgaccga
     3661 ggtggtggcc ggccacccgc cggcccggcc ggtgtcgagg taggtgaggt gccagtcgat
     3721 gcgggcgatg cgccagctca tcgcccgggt gcgctccgac atccgagtcc gggacggggg
     3781 ccgcggcacg gcgacgggat cggacgccgc ggccaggtcg acgccgagct cggccagctc
     3841 gtcgaccacc ccgtcgatga ccgactcgac caccagcgcc tcgagcgacc ccggcgctgg
     3901 ccgggccggc tcgtgcgggc cggtccggcc cggttcctgt cgatccgcat cccaccgatc
     3961 ggacccggac cacccggtcc tggaccgatc ggggtcgggg cgacgtcgcc gggaccacgc
     4021 ggtctgggtg cgcagggccg gcagaccgtc ccagtgacgg gcctcgaccg acggcaggag
     4081 gcccgcggtc tgggtgtcgg ccgacagcag cgacgtggcg tgatcgagtc ggtccctgac
     4141 cggggccgac agtcggtcac gcaactccgt gtgctcggga cgttgcaggg cagccgctgc
     4201 caacgggcca gcggtgctca ggcatcggcg ttcgaccggc ccgtcggccg ccgtggcgga
     4261 gtcgaggcga cggcgcagcg gccggcaccg gtcgtctcgc agccgtccga gccgatcggc
     4321 cagctcccgc accgacggga cggggctcga cgctcccagc tgctcgcccg agcaggcgtc
     4381 gaggaggtcg ccggtcgcca cgacccacgc caccgacacg tcgccgcccc gacggtcgtg
     4441 atcgcgggtc agcgtgtcgg cgtcgcggat gacgtcgaga gcgtcgcgga cgcccgcccc
     4501 ctcgaccgcc cacaggcacc agtcgcagcc gtcgggcggt ctgcggcggt gcgggcactc
     4561 cggcaggtgg ccgtcgaggc gcaacgccgg gtcgagcgcg cccaggtcga gcagcaccgc
     4621 cgcgctcacg gcgtgtccac ccggacggtg cggaacccgt cgccgccggg ggcgggggcg
     4681 acccggtgct cgctctcgtc gatgatcaca gccagcaccg aggtgcgcgg cggcgccgtg
     4741 tccggccacg gcgtccagcc gtcggtgagc acgacgatca gccgggggag cggtctcagt
     4801 gcggccgctg ccgcgatgcc gacgcgcaga tcggtcccgc gacctccgct caggcacagt
     4861 tcgctgagtc gccggaccgt ctgggtgcga gccgccagct cgtcgcagac gacgaccggg
     4921 acgggggcga cgccgccgtg gtgcagcagg gcgtcgagct cggtgaccgc ggcgtcgagc
     4981 cgcgggctgt tcatcgacgc ggacgtgtcg atgacgaccg cgaccgacga ccggagccgg
     5041 cgttggccgg gccgcagcac cccgggtgtg gacgcggcgc ggcgatcggg acggctccag
     5101 tcgggctcgg tgtggcgggt cgtcgcccg
//
LOCUS       DAOQOD010000203         5124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6696_length_5124_cov_11.377195, whole genome shotgun sequence.
ACCESSION   DAOQOD010000203 DAOQOD010000000
VERSION     DAOQOD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_5124_cov_11.377195"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /gene="infB"
                     /locus_tag="PLZ72_14325"
     CDS             <1..1897
                     /gene="infB"
                     /locus_tag="PLZ72_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNA37521.1"
                     /translation="GGGPRPPRRKGRRRRNREELQPMDIPTYTPATAPVPEGVSVIER
                     ASTPQDLGPKLNRTAADVVRFLLQQGEMVTATQSLSDEMIELFAADVGAEIRLVNPGE
                     EQEVELQKVLDLDDDEDLDYESLPHRAPVITVMGHVDHGKTKLLDQIRNANVVAGEAG
                     GITQHIGAYQVVKDGKPLTFIDTPGHEAFTAMRARGADSTDIVVLVVAADDGVMPQTL
                     EAIQHARAAEVPIVVAVNKIDRENADPNRVLQQLSEHDLVPEAWGGDTIVCEISALQN
                     IGIDELLENLTVVAELEDLRADPEGRARGTVLEAHLDMGRGPVATILVQRGTLRVGDP
                     LVAGPAWGRVRALITDKGEQVKEAGPSTPVEVLGLSDVAQAGDDFVVAPDERKAKAVA
                     ETRERWHREASRARDASVMSHGARLEDIFAQIQAGEQAVLNLIVKADVQGSLEAVTES
                     LRKLERPEVKIGFVLRGVGGITESDIQLAATSSATIIGFNVRPGRSARDLAQAEDVEI
                     RTYEIIYKLLEDIEAAMVGMLEPEFEEVVTGEAEVREVFRVPRVGAVAGCMVQNGTIT
                     RGSKVRFLREGTIIWKGEISSLRRFKEDVREVQSGFECGIGLSDFQDLKSGDVIETYD
                     LREIPRS"
     gene            complement(2134..3270)
                     /locus_tag="PLZ72_14330"
     CDS             complement(2134..3270)
                     /locus_tag="PLZ72_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37522.1"
                     /translation="MGECREADRRGNTFASSPRQVLLVATAALALAMSFLLGVSPTSA
                     GAESLPDGPGTITVDQSPVGATGFCLPPFLALTHSVAETPTTFTLKIFASPSLCQPVS
                     ATAAIYAMPLDGSLWPQTLALKQDFIIPGPGVTTVTFHKGCLPAQFDVLTGATPPVIS
                     PTGPWHGPLLFPFALETSLQNSGGDCVGGNCETYTPKNVTVSPDSVAPGGTVTVAGSG
                     VPGTTIEILLQQPPAAAVPTGASVVVPASGSWSVPVTVPASTSPGTWQVVARVEGCDT
                     QTSADLTVTGPGEEPGTPPAENAPTNPPPDGQALASTASNAPAPLPAAAVAGATETQG
                     TSSAADAQAAGLAFTGSTIRLPVLIGVGLLVAGVLLLLKRRRQG"
     gene            complement(3388..>5124)
                     /locus_tag="PLZ72_14335"
     CDS             complement(3388..>5124)
                     /locus_tag="PLZ72_14335"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNA37523.1"
                     /translation="QTAVEDAMGAADRIGGASSTASTAGFASARQHLADVVAAADAPW
                     MIAARAVPGVGANLRSVRESAAAGADLSTVAERLSSEVDYQRLHLDGGGIDLGVLGSF
                     RAPVTEAERALGRADDALSAARSPLVLGPVAERMEELSSRVRRAHSDAVTARLGVEAA
                     PGLLGAAAPRRYLLLLGNPAEARDLGGHLGNWAEVVATDGRLDVVRVGGPYDLFGPTG
                     PDRPFLADRSSFPRSLLEMNPTRFPQNWGATPDLPTVARLAAELYPQSRGGAPLDGVI
                     YADPTAFAAAIGITGPVPVPGTDITVDSDTAAEFLERGQYGLFARESEGDEAVTELVR
                     TALDRLLEGRLPAPDAVASAFGPSVDGGHLKFFSLHESDHELLERLHLDGAIAVTRGD
                     DALAVITRNANPSKIDSYLKRSIDQRVTWDPDSGDVRTRVTVELTNTAPATGLPQVVG
                     LAPPGSPAGTNRTELAVLTPLRATGATVDGAATAIGSRDDLDGLHRHTLQVDLAPGQT
                     RTVAFDLSGRLDPGDYRLRWIAQPLVNPDDARLVITSSGRPFRGGADSGTVDLTEDLT
                     GGGRNITVRMQS"
BASE COUNT          763 a         1757 c         1887 g          717 t
ORIGIN      
        1 cggcggcggt ccgcggccac cgcggcgcaa gggacgccgg cgtcgcaacc gcgaagagct
       61 ccagccgatg gacatcccca cctacacgcc ggccaccgcg ccggtcccgg agggggtgtc
      121 ggtcatcgag cgggcgtcca cgccccagga cctcggtccc aagctgaacc gcaccgcggc
      181 cgacgtcgtg cgcttcctgc tccagcaggg cgagatggtc accgcgaccc agtccctcag
      241 cgacgagatg atcgagctct tcgccgccga tgtcggcgcc gagatccgac tggtcaaccc
      301 cggtgaggag caggaggtcg agctgcagaa ggtgctcgac ctcgacgacg acgaggacct
      361 cgactacgag tcgttgcccc atcgggcgcc ggtgatcacc gtgatgggtc acgtcgacca
      421 cggcaagacc aagctgctcg accagatccg caacgcgaac gtcgtcgccg gcgaggcggg
      481 cggcatcacc cagcacatcg gtgcgtacca ggtcgtcaag gacggcaagc cgctgacgtt
      541 catcgacacc cccggccacg aggcgttcac cgccatgcgg gcccgtggtg ccgactccac
      601 cgacatcgtc gtgctggtcg tcgcagcgga tgacggcgtg atgccccaga cgttggaggc
      661 catccagcac gcgcgtgccg cagaggtccc gatcgtggtg gccgtgaaca agatcgaccg
      721 cgagaacgcc gatcccaacc gcgtcctgca gcagctgtcc gagcacgacc tggtccccga
      781 ggcctggggc ggcgacacga tcgtctgcga gatctccgca ctgcagaaca tcggtatcga
      841 cgagctgctc gagaacctca ccgtcgtcgc cgagctcgag gacctgcgtg ccgatcccga
      901 gggccgggcc cggggaacgg tgctcgaagc ccacctcgac atgggtcgcg gtcccgtcgc
      961 cacgatcctg gtccagcggg gcaccctgcg agtcggcgac ccgctggtgg ccggccccgc
     1021 gtggggacgc gtccgggcac tgatcaccga caagggagag caggtcaagg aggcgggtcc
     1081 gtccacgccg gtcgaggtgc tcggcctgtc cgacgtcgcc caggccggcg acgacttcgt
     1141 cgtcgccccc gacgaacgca aggcgaaggc cgtggccgag acccgcgagc gctggcaccg
     1201 tgaggcgtcg agggcgcgtg acgcgtcggt catgtcccac ggcgcccgcc tcgaggacat
     1261 cttcgcccag atccaggccg gcgagcaggc ggtcctcaac ctcatcgtca aggccgacgt
     1321 ccagggatcg ctcgaggctg tcaccgagag cctccgcaag ctggagcggc ccgaggtcaa
     1381 gatcggcttc gtgctgcgcg gcgtcggcgg gatcaccgag tccgacatcc agctggcggc
     1441 cacctccagc gccacgatca tcggcttcaa cgtccgtccc gggcgttccg cccgtgacct
     1501 ggcccaggcc gaagacgtcg agatccgcac ctacgagatc atctacaagc tcctcgagga
     1561 catcgaagcg gccatggtcg gcatgctcga gcccgagttc gaagaggtcg tcaccggcga
     1621 ggccgaggtc cgggaggtgt tccgggtgcc ccgcgtcggg gccgtggccg gctgcatggt
     1681 gcagaacggc accatcaccc ggggctccaa ggtccgcttc ctgcgagagg gcacgatcat
     1741 ctggaagggc gagatctcgt cgttgcgccg cttcaaggag gacgtccgag aggtccagtc
     1801 cggcttcgag tgcggcatcg ggctgtcgga cttccaggac ctcaagtcgg gcgacgtcat
     1861 cgagacctac gacctgcggg agatcccccg cagctgatgc cgggcaggac gccggggtgg
     1921 gtgcggtacc tcccggcgtc cgcggttcca ttcgtacgcg gttcggactt ccgcctcggc
     1981 cgagcctctc ccgccgtgct ggtcgcgctg agcgaacgcc gggtggtccc gcccttcgcg
     2041 ggcggccgtg cgaggtgatc tggtcctgct gagcccgctg gtcgaccgat cgcggccgtc
     2101 agctgtgcac cgctgtccga ccgcgtgggg cgctcagccc tggcggcgac gcttcagcag
     2161 gagcaggacg ccggcgacca ggagaccgac cccgatcagg accgggagtc ggatcgtcga
     2221 cccggtgaag gcgaggcctg cggcctgggc gtcggctgcg ctcgaggtgc cctgcgtctc
     2281 ggtcgctccg gcgaccgcag ccgccggaag gggtgccggt gcgttcgatg cggtgctggc
     2341 gagcgcctgt ccgtcgggcg gcgggttggt cggcgcgttc tccgcgggcg gtgtccccgg
     2401 ctcctcaccc gggcccgtga ccgtgagatc ggcggaggtc tgggtgtcgc agccctcgac
     2461 ccgggcgacg acctgccagg tgccgggact cgtgctcgca ggaacggtca ccggaaccga
     2521 ccaggacccg ctggcgggga cgacgacgct cgcacccgtg ggcacagcag cagcaggtgg
     2581 ttgctggagc aggatctcga tcgtggtgcc cgggactccg ctgccggcca ccgtcaccgt
     2641 gccgccgggg gccaccgaat cggggctgac ggtcacgttc ttgggggtgt aggtctcgca
     2701 gttgccgccg acgcagtcgc cgccggagtt ctgcaacgac gtctcgaggg cgaacgggaa
     2761 aaggagcggt ccgtgccacg gtccggtggg cgagatcacc ggtggcgtgg ccccggtgag
     2821 gacgtcgaac tgggcgggca ggcagccctt gtggaacgtg acggtggtga cgcccggacc
     2881 cgggatgatg aagtcctgct tgagcgccag ggtctggggc cacagcgagc cgtcgagggg
     2941 catggcgtag atcgcggcgg tggccgacac gggctggcac aacgacggag aggcgaagat
     3001 cttgagcgtg aaggtcgtcg gggtctcggc cacgctgtgg gtcagggcga ggaacggcgg
     3061 gaggcagaag ccggtcgccc ccaccgggga ctggtccacg gtgatcgttc cgggcccgtc
     3121 gggcaggctc tctgccccgg cggaggtggg ggagacgccg aggaggaacg acatggccag
     3181 ggcgagcgcg gcggtcgcca cgagcaggac ctgacgcggg gatgacgcga aggtgttccc
     3241 tcgtcggtcc gcttcccggc actctcccat gtgaatgaac cctccagctc ggcaccgacg
     3301 gtgggagtcg ctcctggctc cgacgtcaga cctcccacaa gtgtgacgtc gtcaggctac
     3361 cggagtccac ccttctgtga acatctttca gctctgcatg cggaccgtga tgttgcggcc
     3421 accaccggtg aggtcctcgg tgaggtcgac cgttccgctg tcggccccac cgcggaacgg
     3481 ccgaccggac gaggtgatga ccagccgggc gtcgtcgggg ttgaccagcg gctgggcgat
     3541 ccagcggagg cgatagtcgc cggggtccag gcggccggag aggtcgaacg cgacggtccg
     3601 ggtctgcccg ggggcaagat ccacctgcag ggtgtggcgg tggagtccgt cgaggtcgtc
     3661 gcgggagccg atggcggtcg ctgctccgtc cacggtcgcg ccggtcgcac gcagaggcgt
     3721 gagcacggcg agctcggtcc ggttcgtccc cgccggcgac ccgggtggcg ccaggccgac
     3781 cacctggggg agacccgtgg ccggggcggt gttggtgagc tccaccgtca cccgggtccg
     3841 cacgtctccg gagtcgggat cccacgtgac ccgttggtcg atcgagcgct tcaggtagga
     3901 gtcgatcttg gacgggttgg cgttgcgggt gatcactgcc agagcgtcgt ccccgcgggt
     3961 gacggcgatc gcgccgtcga ggtgcagtcg ctcgagcagc tcgtggtccg actcgtgcag
     4021 ggagaagaac ttgagatggc ccccgtcgac cgacggaccg aacgcgctcg ccacggcgtc
     4081 cggagcgggg agccggccct cgagcaggcg gtcgagagcg gtccggacga gctcggtgac
     4141 cgcctcgtca ccctctgact cgcgggcgaa caggccgtac tgtccccgtt ccaggaactc
     4201 tgccgccgtg tccgaatcga cggtgatgtc ggtgccggga accggcaccg gcccggtgat
     4261 cccgatcgcc gcggcgaacg cggtcggatc ggcgtagatg acgccgtcga gcggcgcacc
     4321 gccccgggac tgcgggtaca gctcggccgc caggcgggcg acggtgggca gatcaggggt
     4381 cgctccccag ttctggggga accgggtcgg attcatctcg agcagcgatc gggggaacga
     4441 ggatcgatcg gccaggaacg gccggtcggg ccccgtgggt ccgaacaggt cgtacgggcc
     4501 acccacacgc accacgtcca ggcgtccgtc ggtggccacc acctcggccc agttccccag
     4561 gtggccaccc aggtcacgag cctcggccgg gttgcccagc agcaggaggt accgacgcgg
     4621 tgcggccgct ccgagcagtc cgggcgcggc ctcgaccccc agtcgggcgg tcacggcgtc
     4681 ggagtgggcc cgtcgcaccc gcgacgacag ctcctccatg cgctccgcca ccggtccgag
     4741 caccagggga gaccgggcgg cgctgagggc gtcgtcggcg cgtcccaggg ccctctcggc
     4801 ctcggtcacc ggcgcccgga acgagccgag gactcccagg tcgatcccgc cgccgtcgag
     4861 gtgcaggcgt tggtagtcga cctcgctcga caacctctcg gcgacggtcg acaggtcggc
     4921 tcccgcagca gcggactcac ggaccgatcg gaggttggcc ccgacgcccg gaacggctcg
     4981 ggccgcgatc atccacgggg cgtccgccgc ggcgacgacg tcggccaggt gctggcgcgc
     5041 cgaggcgaac ccggcggtcg aggcggtgct cgacgcgccg ccgatgcggt ccgcggctcc
     5101 catcgcgtcc tcgacggcgg tctg
//
LOCUS       DAOQOD010000204         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6745_length_5099_cov_15.549564, whole genome shotgun sequence.
ACCESSION   DAOQOD010000204 DAOQOD010000000
VERSION     DAOQOD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_5099_cov_15.549564"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2370)
                     /locus_tag="PLZ72_14340"
     CDS             complement(<1..2370)
                     /locus_tag="PLZ72_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNA37524.1"
                     /translation="MARIPRPLAALVGLGLLASLTVACSGTARDAPPLLLPVNSAPTT
                     VYDAKGRPITVLRDENRTSIPLEQIPNITRTAVVAIEDSRFWSHRGVDPRAIARAASS
                     NAEEGETGQGGSTITQQYVKTALLSPEKTLQRKLEEASMALSIERNYSKELILELYLN
                     TIYFGDGAYGIDAAARSYFGIPASELDLAQSALLAGLIQAPSRYDPRRHPEAAFKRRN
                     VVLARMLELGDITQTQHDTALMTPVTLAPPQPLPEQVPYPAAHFVDAVKEYLLKDSTV
                     LGDTQGERYNNLYRGGLQIHTTIDLDLQAQAEQSIAQVLPRQGVDPKMPDAALVSIDP
                     ATGEVKAMVGGRDYFGTSDYRQTNLARGAGRQTGSAFKPIVLAAALDNGVSPEKLFDA
                     PSAQVHKLSDGTTWRVKGGGIGRGTMAQCTVVSSNTCYANIVLDPEVGAQRSVDMAKA
                     LGIVDTKLSAVPSAVLGTNNATVQDMASVYATFANQGVYVPPVLVNRIDRPDGTMLYQ
                     HDHTQKKVLEPEVAAQISTILPGVIEGGTGTRANIGRPAAGKTGSSQNNVDGWFCGYV
                     PQLATAVWVGFALPRAGKDGKLRPVPMTSPNTRITVFGGTYPAQIWASFMSKAVRDLP
                     VEPLVDPLAAAPTTTTVPPSNEALLSPVTVPRDAKVPDVGGKDTRKAIAAVRSAGFVA
                     VRVDATVAGVGPGIVTGQSPAPGAAAAAGSTVFIESTPGTFVPPDAVPDVIGFGTGQA
                     RQQLESLGFTVTLEPLAAPEAARRKDGQPFEAGQVWRSTPAAGNAAVDGK"
     gene            2529..3830
                     /gene="tyrS"
                     /locus_tag="PLZ72_14345"
     CDS             2529..3830
                     /gene="tyrS"
                     /locus_tag="PLZ72_14345"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNA37525.1"
                     /translation="MTGEVRPHGGGVIDDLTARGLVQDHTDLDALRDRLDAGPTPTYC
                     GFDPTADSLHIGNLQSIMLLRRFQDAGHPPIALVGGATGMIGDPSGRSSERTFLDTDV
                     LDANRAAIGNQLARFLSFDGATAATVVDNRTWTESVSVLEFLRDVGKHVTINTMLAKD
                     SVRSRMERESGISYTEFSYMLLQANDFDVLHQSHACDLQVAGSDQWGNITAGVDLVRR
                     RRGEAVHGLTAPLIVRSDGTKFGKSEGENIWLDAARTSPYRLYQYFLNVGDEDVEPLL
                     LRLTTVAVEECRRIAVEHAAAPERRLGQRALARALTSVVHGSDAIAAIEEASAVLFGD
                     QPITSASVGALDLLAAEVPSSTASRGDLLGSDPVDLVADGEVGLARSKGEVRRNLQGY
                     SVNQVKLSDRPGAPLAESDLLHGRYILLARGRRTHHLVRVG"
     gene            4285..>5099
                     /locus_tag="PLZ72_14350"
     rRNA            4285..>5099
                     /locus_tag="PLZ72_14350"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          813 a         1635 c         1828 g          823 t
ORIGIN      
        1 cttgccgtcg accgcagcgt tcccggccgc cggtgtgctg cgccagacct gacccgcctc
       61 gaagggctgg ccgtccttgc gccgcgcggc ctccggtgcc gcgagcggct ccaacgtgac
      121 ggtgaacccg agggactcga gctgctggcg ggcctggccg gtcccgaagc cgatgacgtc
      181 gggaaccgcg tcgggcggga cgaaggtgcc gggggtcgac tcgatgaaca cggtggaacc
      241 ggccgcggcc gccgctcccg gagccgggct ctggccggtg acgatccccg gacccacccc
      301 ggcgacggtg gcgtccacgc gcacggcgac gaacccggcc gagcgcaccg cggcgatcgc
      361 cttgcgggtg tccttgccgc ccacgtcggg gaccttggcg tcgcggggga cggtgaccgg
      421 gctgaggagc gcctcgttcg agggcggaac cgtcgtcgtg gtgggtgcgg ccgccaaggg
      481 gtcgacgagg ggctccaccg gcaggtcgcg cacggccttg ctcatgaacg acgcccagat
      541 ctgcgccggg taggtgccgc cgaagaccgt gatccgcgtg ttgggagacg tcatcgggac
      601 cggtcgcagc ttcccgtcct tgccggcccg gggcagggcg aagccgaccc acaccgcggt
      661 ggccagctgg gggacgtagc cgcagaacca gccgtcgacg ttgttctgcg acgacccggt
      721 ctttcccgct gccggtcgac cgatgttggc ccgggtgccg gtgccgccct cgatcacgcc
      781 gggcaggatc gtcgagatct gcgccgccac ctcgggctcg agcaccttct tctgggtgtg
      841 gtcgtgctgg tagagcatgg tgccgtcggg tcggtcgatc cggttgacga gcaccggcgg
      901 gacgtacacc ccctggttgg cgaaggtggc gtacaccgac gccatgtcct gcaccgtcgc
      961 gttgttggtg ccgagcaccg ccgagggcac cgccgacagc ttggtgtcga cgatgccgag
     1021 agccttcgcc atgtcgaccg atcgttgggc gccgacctcg gggtccaaca cgatgttggc
     1081 gtagcaggtg ttggacgaca ccaccgtgca ctgcgccatg gtcccgcggc cgatccctcc
     1141 gcccttgacc ctccaggtgg tgccgtcgga cagcttgtgc acctgggcgg acggagcgtc
     1201 gaagagcttc tcgggtgaga cgccgttgtc gagcgcggcg gcgagcacga tgggcttgaa
     1261 cgcggagccg gtctgacggc cggcgccgcg ggccaggttg gtctggcggt agtcggacgt
     1321 tccgaagtag tcgcgtccgc cgaccatcgc cttgacctca ccggttgccg ggtcgatcga
     1381 caccagcgcg gcatcgggca tcttggggtc gacgccctgt cggggcagca cctgggcgat
     1441 cgactgctcg gcctgggcct ggaggtcgag gtcgatcgtg gtgtggatct ggaggccacc
     1501 tcggtagagg ttgttgtagc gctcgccctg ggtgtcgccg agcaccgtcg agtccttcag
     1561 caggtactcc ttgacggcat cgacgaagtg ggcagccggg tacgggacct gttcggggag
     1621 cggctggggc ggcgccagcg tcaccggcgt catcagcgcg gtgtcgtgct gggtctgggt
     1681 gatgtcgccg agctcgagca tccgggcgag caccacgttg cgccgcttga acgcagcttc
     1741 ggggtggcgt ctggggtcgt agcgggacgg ggcctggatc aggccggcga gcagggcgga
     1801 ctgggccagg tcgagctcgc tggcggggat cccgaagtac gagcgggccg ccgcgtcgat
     1861 gccgtaggcg ccgtctccga agtagatcgt gttgaggtag agctcgagga tgagctcctt
     1921 ggagtagttg cgctcgatcg acagcgccat cgacgcctct tcgagcttgc gttgcagcgt
     1981 cttctcgggt gacagcagag cggtcttcac gtactgctgg gtgatggtcg agccgccctg
     2041 gccggtttcg ccctcctcgg cgttcgagct ggcggcccgt gcgatggcgc ggggatccac
     2101 gccgcgatgt gaccagaacc gggagtcctc gatcgcgacc accgccgtgc gggtgatgtt
     2161 ggggatctgc tccaggggga tgctggtgcg gttctcgtct cgcagcaccg tgatgggccg
     2221 ccccttggcg tcgtagacgg tggtcggcgc cgagttgacc ggtagcagca gtgggggagc
     2281 gtcgcgggcg gttccgctgc atgccacggt cagcgacgcc agcagaccca gtccgacgag
     2341 ggccgcgagg gggcggggga tccgagccat gaccgatcca gtgttgccga cccgccgccc
     2401 gaactggtgc cagccggctg agagagcagc ggtacgctct caggtcgccc gtcggctccg
     2461 ttggcgacgg gtccgacgac gtgggcgcag gaggtcgatc cgacccggcc cggaggagag
     2521 accgagcgat gaccggagag gtccgacccc acggcggcgg ggtgatcgac gacctgaccg
     2581 cacgaggcct ggtccaggac cacaccgacc tcgacgcgtt gcgcgaccgc ctggacgccg
     2641 gtcccacacc cacctactgc gggttcgacc cgacggcgga ctcgctgcac atcggcaacc
     2701 tccagtcgat catgctgctg cggcgcttcc aggacgccgg ccacccgccg atcgccctgg
     2761 tgggcggggc caccggcatg atcggcgacc cctcggggcg cagctcggag cggaccttcc
     2821 tcgacaccga cgtgctcgac gccaaccggg ctgccatcgg gaaccagctg gcacgcttct
     2881 tgtccttcga cggcgcaacc gcggcgacgg tggtggacaa ccggacctgg accgagtcgg
     2941 tcagcgtcct cgagttcctc cgcgacgtcg gcaagcacgt cacgatcaac acgatgctgg
     3001 ccaaggactc ggtgcgcagc cggatggagc gcgagtcggg gatctcctac accgagttca
     3061 gctacatgct cctgcaggcc aacgacttcg acgtcctgca ccagagccac gcgtgcgacc
     3121 tccaggtggc gggatcggac cagtggggca acatcaccgc gggcgtcgac ctggtccgtc
     3181 gccgccgcgg cgaggcggtc cacggcctga ctgcaccgct gatcgtccgc tccgacggca
     3241 ccaagttcgg caagtccgag ggcgagaaca tctggctcga cgcggcccgg acctcgccgt
     3301 accggctgta ccagtacttc ctcaacgtcg gtgacgagga cgtcgagccg ctcctgctcc
     3361 ggctcaccac cgtggcggtc gaggagtgcc gccgcatcgc ggtcgagcac gccgccgccc
     3421 cggagcgcag gctcggtcag cgggcgctgg cgcgggcgct gacctcggtg gtgcacggat
     3481 ccgacgccat cgccgcgatc gaggaggcct ctgcggtgct gttcggagat cagccgatca
     3541 cctcggcctc ggtcggtgcg ctggacctgc tcgcggccga ggtcccgtcc agcaccgctt
     3601 cccgcggcga cctcctcggc tcggacccgg tcgacctcgt ggccgacggg gaggtcgggt
     3661 tggcccgctc caagggagag gtgcgccgga acctgcaggg atactcggtg aaccaggtga
     3721 agctgtcgga ccggccgggt gctccgctgg ccgagtcgga cctgctgcac gggcggtaca
     3781 tcctgctcgc ccgtgggcgc cgcacccacc acctggtcag ggtggggtga gcagtcctcc
     3841 gtgccgccgt tcggccgacg gacggaccgc cgctgaggga cgtgacgggc gtctcacgac
     3901 atcgagttga ccccggccac cgggtgccat attctctcac ttcgccccgc acggcagctt
     3961 cggcaccgga acggggaacg gaacccctgg ttcgcacggt caccgtggca ccacgttcca
     4021 gctcgagccc ccctcgtgga gctcaggcgt tccttgacaa cggaagacag gatgccaaaa
     4081 ggcgagtgcg ggagactacc cgtgggttca cacccgcgag cagtcgaccc gatcaattca
     4141 gatatttatg cactttccgg atgatgatct gccggtcttc ggatcggtgg accaacgcca
     4201 gtctgtgcca gtcggtgatc aggttcaggc ctgccaccgg ctctccgatc gacggacgag
     4261 ctcttcggag cagatcctga ggtcttgatg gagagtttga tcctggctca ggacgaacgc
     4321 tggcggcgtg cctaacacat gcaagtcgaa cggggtccat gggagcttgc tcccggaaga
     4381 cctagtggcg aacgggtgag taacacgtga gaaacctgcc ctggagtggg gaataactcg
     4441 gggaaactcg agctaatacc gcataccctc ggatggtcgc atggccgacc gaggaaagat
     4501 tcatcgcttc aggagggtct cgcggcctat cagctagttg gtggggtaac ggcctaccaa
     4561 ggcaacgacg ggtagctggt ttgagagaac gatcagccac actgggactg agacacggcc
     4621 cagactccta cgggaggcag cagtggggaa tcttgcgcaa tgggcgaaag cctgacgcag
     4681 caacgccgcg tgcgggatga cggccttcgt ggttgtaaac cgctttcagc agggacgaaa
     4741 atgacggtac ctgcagaaga agccccggct aactacgtgc cagcagccgc ggtaatacgt
     4801 agggggcaag cgttgtccgg attcattggg cgtaaagagc tcgtaggcgg ttcagtaagt
     4861 cgggtgtgaa agctccgagc tcaactcgga gaggccaccc gatactgctg tgacttgagt
     4921 ccagtagagg agcgtggaac tcctggtgta gcggtggaat gcgcagatat caggaagaac
     4981 acctgcagcg aaggcggcgc tctgggctgg aactgacgct gaggagcgaa agcgtgggta
     5041 gcaaacagga ttagataccc tggtagtcca cgccgtaaac gttgggcact aggtgtggg
//
LOCUS       DAOQOD010000205         5073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6808_length_5073_cov_9.578717, whole genome shotgun sequence.
ACCESSION   DAOQOD010000205 DAOQOD010000000
VERSION     DAOQOD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_5073_cov_9.578717"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1709)
                     /locus_tag="PLZ72_14355"
     CDS             complement(69..1709)
                     /locus_tag="PLZ72_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA37526.1"
                     /translation="MVRNTEVRARSRAQLGAASVHVERGGRPVLVDVSVSVSAGTRLG
                     VVGENGRGKSTLLHVLVGTLRPDSGTVSRIGSIGVAEQELAAGDARTVGELIDVELAD
                     ARAALKALDEAAAAMAAEVEGADDEYAAALDAATALDAWDADRRVDLALDALGAVRDR
                     SRALAQMSVGERYRVRLACLLGAQHDFLLLDEPTNHLDEHGLDFLTAALRDHPGGVVL
                     VSHDRVLLADVATKILDLDPSIDGRPRVYGDGYEGYRAGRSAAMARWEQDYDQHLAEE
                     ARLADDLSAAQNRLRSSWRPPKGTGKHQRATRAPSLVRAVNRRIDDLADHAVSRPVAP
                     LRFHMPELATLSGVTLVRAEEVAVDGRLTGPVTVALESGDRLLITGPNGAGKSTLLAV
                     LAGQLEADQGSVRVAANARIGFVAQETPSADRRRVHDIYEARMHQLRHLGLDATVPLR
                     SLGLLSAADTARPLAELSMGQQRRLDLALALAGRPHALLLDEPTNHLSIALVDELTEA
                     LDATAAAVVVVTHDRQLRRDLGHWPELVIPGRNEAHKA"
     gene            1965..2510
                     /locus_tag="PLZ72_14360"
     CDS             1965..2510
                     /locus_tag="PLZ72_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37527.1"
                     /translation="MEHGRTLTFTPVNHPNRQVRQVGFDLSDTYVEQCWSAVIGPSAV
                     VLLRRMPELWRHEVPAKIPADELSRSIGLGGGTGPNSRLVAITDRMVKFGLVRRSGGP
                     DRFDVFLEVAPLQARQLERVPEWTRATHERLFSAHLEGFDGVAEHRAKVTNAAVDLDR
                     FRDRATHSDRTPPAARQALGR"
     gene            2650..>5073
                     /gene="mobF"
                     /locus_tag="PLZ72_14365"
     CDS             2650..>5073
                     /gene="mobF"
                     /locus_tag="PLZ72_14365"
                     /inference="COORDINATES: protein motif:HMM:NF020332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HNA37528.1"
                     /translation="MKFTVTPLGGGRADTARVVDSIVRYLNPPAAKTPGAGGPAPSGA
                     EGAEQYYADKGEEPGRWMGRHAAATGLAGAVQRHDFASVLAGRDPHTGERLITAQGSA
                     GRRPTLGAGAHSRTGAHGERLYDVADAAAVLGVTHREVERMLDAGTRLVHAALPPTAG
                     QGSTEDPAGHPVGAAETEPGAAYLVPLVDEDGSRWVSEEELARCQQARDEGVTPEQVR
                     ALGGPEDQLSISDAARLAGVTNRYLRGVARYHEDHSVEIAQSVAAGRQPRRAYLEAHR
                     GTKGRWLVTREHLAEFLERRRPPAVRVAYDLTLTTEKSLGVLALLGNDTTRRAVLGSI
                     QAGNDWALGWIEERAVGRIDGQPVAAQGLMVASFRHLTSRALDPFPHHHNVVLNTVQV
                     PDGSHRSLWSRCLYRNAQAASALATAEMRHQLASQLDVRWRPGRKSGWEIDGIDQAVV
                     SEFSKRRNEIDDALAELETEIGRGAHPGEVEHIVLRTRPAKNHTPADKLIADWRARAE
                     RAGLTSDDLDRLAPPIREPDTQRDSQPDSQRDRTETVYRLLAADDGICAGGSVFARAD
                     AIAAVANVGVPDPDGVAQPLLGGATQVLELTDGFLASGHVVALTDGDEPLFTTRAMLE
                     MQNRIAARFRAGRHEGTHHVPDHHLATALAGHPHLTGEQRDLVRSWCQSGHRFQPAIG
                     RAGTGKTTTVAACADAWRAAGYRVVGAAVKGEAARTLAAATGIECETVAWYLAHTDPE
                     HPPLDARTILVIDEASTLHDRALDTFMTMAADTGASIRFIGDPAQHGAIAAGGMFRVL
                     CERHPAITPE"
BASE COUNT          807 a         1817 c         1725 g          724 t
ORIGIN      
        1 cctatccggt tggcgagaaa tcctcgagat tcttcaaccg gatacccagc gggatagaca
       61 gcgcgatatc acgccttgtg ggcctcgttt cgtccaggaa tcaccagttc tggccagtgg
      121 ccgaggtcgc ggcggagctg gcggtcatgg gtcacgacga cgaccgctgc ggccgtggcg
      181 tcgagggcct cggtgagctc gtcgacgagc gcgatcgaca ggtggttggt gggttcgtcg
      241 aggagcaggg cgtggggtcg gccggcgagg gcgagggcga ggtcgagacg gcgctgctgg
      301 cccatggaga gctcggcaag gggtcgagcg gtgtcggcag ccgagagcag cccgagggac
      361 cgcaacggca cggtcgcatc gagtcccagg tgacggagtt gatgcatacg ggcttcgtag
      421 atgtcatgga ctcggcgtcg gtcagctgac ggggtctcct gggcgacgaa gccgatgcga
      481 gcgttcgccg cgaccctcac cgagccctgg tccgcttcga gctgtccggc aagcaccgcg
      541 agcagggtgg acttgccggc cccgttcgga ccggtgatca ggagacggtc gccggattcg
      601 agcgcgacgg tgaccggacc cgtgagccgg ccgtcgaccg cgacctcctc ggctcgcacg
      661 agcgtcacgc cggacagcgt tgccagttcg ggcatgtgga accgcagcgg cgccaccggt
      721 ctcgatacgg cgtggtcggc gaggtcgtcg atccgtcggt tcaccgcccg aaccagagac
      781 ggtgcccgag tggcgcgctg gtgcttgccg gtgcccttcg gtggacgcca gctcgaacga
      841 agccggttct gcgccgccga aaggtcgtcg gcgaggcggg cctcctcggc caggtgctgg
      901 tcgtagtcct gctcccagcg ggccatcgcc gccgaacgac cggcccggta gccctcatag
      961 ccatcgccgt agacgcgggg ccgcccatcg atgctgggat ccaggtcgag gatcttggtc
     1021 gcgacatcgg cgagcaacac ccggtcgtgg ctgaccagga ccaccccgcc gggatggtcg
     1081 cgcagcgcgg cggtcaagaa gtcgaggccg tgctcgtcga ggtggttggt gggctcgtcg
     1141 aggagcaaga agtcgtgctg agcaccgagc aggcacgcca ggcgcacccg gtagcgctcc
     1201 ccgaccgaca tctgagcgag tgcgcggctg cggtcgcgca ccgcgccgag cgcgtcgagc
     1261 gccaagtcaa cgcgtcggtc cgcatcccac gcgtcgagcg ccgtcgcagc atcgagcgcc
     1321 gccgcgtact cgtcgtcagc accctcgacc tccgcggcca tggccgcggc agcctcgtcg
     1381 agggccttca acgcggcgcg ggcatcggcc agctccacgt cgatcagctc accgacggtt
     1441 cgcgcgtctc cagcagccag ttcctgctca gcgacgccga tgctgccgat ccggctgacc
     1501 gtccccgagt cagggcgcag cgtccccacg aggacgtgca gcagcgtcga cttgccgcga
     1561 ccgttctcac ccaccacgcc cagacgggtg cccgccgaga ccgacaccga cacgtcgacc
     1621 aggaccggcc gaccccctcg ttccacgtgc accgacgcgg cgccgagttg ggcgcgcgac
     1681 cgcgcccgaa cctctgtgtt gcgaaccaag accagttcct tcccagtggg tgaccatcac
     1741 cgccaaccgg cgcggccaca cgcgatccac gaacgagcga cccctccgtc agggttcgcc
     1801 gatcaacccg gacgcgacac gcgtcccagg ggtccgtgga tcaaaggaag atcgactgca
     1861 acatgacagc caggctacca acgaatctgg ctcccaccgg gcaggttcgc gggcgctgtc
     1921 caccttgagc gcgggattcg gacccgttcg cggcttacct gggcatggaa cacggccgaa
     1981 ctctcacctt cacgcccgtc aaccacccga accgccaggt tcgacaggtc ggcttcgacc
     2041 tttcggacac ctacgtcgag cagtgctggt ctgctgtcat cggaccgagc gcggtggtgc
     2101 tgttgcgccg aatgcccgag ctgtggcgcc acgaggtccc ggccaagatc cccgcggacg
     2161 agctgagccg ctcgatcggg ctcgggggcg ggaccgggcc gaacagccgg ctcgtggcga
     2221 tcaccgatcg gatggtcaag ttcgggctcg tccgccggag tggcggcccg gaccgcttcg
     2281 acgtgttcct cgaggtcgca ccattgcagg cccgacagct cgagcgggtg cccgagtgga
     2341 cccgggccac ccatgagcgg ttgttctcgg ctcacctcga gggcttcgac ggtgtcgccg
     2401 agcaccgagc gaaggtcacc aacgcggcgg tcgatctgga tcggtttcgt gaccgagcca
     2461 cccactccga ccgcacccca ccagcagcgc gtcaggccct tgggcgatga gcctcaggcg
     2521 ccgtcccggc tctcccggaa gcccggtcat cgcgggaacc cacgtcggtt ctcaacggca
     2581 ggtctggcgc gcacacattg acaggttccc cttcggggaa cctgttggct atccggttgg
     2641 cagctcgtcg tgaagttcac cgtcaccccg ctcggcggtg gccgggccga cacggcccgg
     2701 gtcgtggact cgatcgtccg ctacctgaac ccgcccgccg ccaagacccc cggggcgggc
     2761 ggccccgctc catcgggggc agagggcgcg gagcagtact acgcggacaa gggcgaggaa
     2821 ccgggtcggt ggatgggccg ccacgccgcg gccaccggcc tggcgggggc ggtgcagcgc
     2881 catgacttcg cgtcggtgct cgctggccgt gatccccaca ccggggaacg gttgatcacc
     2941 gcgcagggct cggccggtcg acgcccgacg ttgggtgcag gggcacactc gcggaccggc
     3001 gcccacggcg agcggctcta tgacgtcgcg gacgctgccg cggtcctcgg ggtcacgcat
     3061 cgcgaggtcg agcggatgct cgacgccggg acccgcctgg tacacgcggc actcccaccg
     3121 acggccggtc aaggctcaac cgaagatccc gctggtcatc cggttggcgc tgccgagacc
     3181 gagcccggcg cggcgtatct ggtccctttg gtcgatgagg acgggtcccg atgggtgagc
     3241 gaggaagagc tggctcgctg ccaacaggcc cgtgacgagg gcgtgacacc cgagcaggtc
     3301 cgtgccctcg gtggccccga ggatcagctg tcgatcagcg acgcagcccg cctcgccggt
     3361 gtgaccaacc gctacctgcg cggggttgcc cgttaccacg aggaccactc ggtggagatc
     3421 gcccagtcgg tcgccgcggg tcgtcagcct cgccgggcgt acctggaagc gcatcggggc
     3481 accaagggcc gctggttggt cacccgtgag cacttggccg agttcctcga acgacgacga
     3541 ccaccggcag tgcgcgtcgc ctacgacctg acgctcacca ccgagaaatc cctcggggtg
     3601 ttggccctgc tcggcaacga cacgacccga cgggcggtgc tggggtcgat ccaggccggc
     3661 aacgactggg cgctcggctg gatcgaggag cgtgccgtgg ggcgcatcga cggccaacca
     3721 gtagcggcgc aggggttgat ggtggcgtcg ttccggcacc tcacctcacg agcactggac
     3781 ccgttccccc accatcacaa cgtcgtgctc aacaccgtcc aggttcccga cggttcccac
     3841 cggtcgctgt ggtcacgctg cctgtaccgc aacgcccaag ccgcgtccgc gttggcgacc
     3901 gcggagatgc gccaccagct cgccagccag ctcgatgtgc gatggcggcc cggccgcaag
     3961 tccggatggg agatcgacgg catcgaccag gccgtcgtgt cggagttctc gaagcgccgc
     4021 aacgagatcg acgacgccct cgccgagctc gaaaccgaga tcggccgtgg cgcgcatccc
     4081 ggcgaggtcg aacacatcgt gttgcgtaca cgccccgcca agaaccacac ccccgccgac
     4141 aagctcatcg ccgactggcg ggcacgcgcc gaaagagccg gtctgaccag cgatgatctc
     4201 gaccggctcg ccccacccat ccgtgaaccg gatacccaac gggatagcca accggatagc
     4261 cagcgggata ggacagagac cgtctaccga ctcctcgcag ccgatgacgg gatctgtgcc
     4321 ggtgggtcgg tgttcgcccg agccgacgcc atcgccgcgg tcgcgaacgt cggggtcccc
     4381 gatcccgacg gtgtcgccca accactcctc ggtggcgcga cccaggtcct cgagctgacc
     4441 gacgggttcc tcgcgtctgg tcatgtggtc gcgctcaccg acggcgacga gccgctgttc
     4501 accacccgag cgatgctcga gatgcagaac cgcatcgcag cacggttccg ggccggtcgc
     4561 cacgaaggca cccaccacgt ccccgaccac cacctcgcca ccgcgctcgc cggccacccg
     4621 catctgaccg gtgaacagcg cgacctggtt cggtcgtggt gtcagagcgg tcaccggttc
     4681 caacccgcga tcggccgggc cgggaccggc aagaccacca ccgtcgccgc gtgcgctgat
     4741 gcgtggcggg ccgccggcta ccgggtcgtc ggtgccgcgg tgaagggcga ggccgctcgc
     4801 accctcgccg ctgcgaccgg gatcgagtgc gagaccgtcg cctggtacct cgcccacacc
     4861 gaccctgagc acccgcccct cgacgcccgc acgatcctcg tcatcgacga agcatcgacg
     4921 ctgcacgacc gggcgctcga caccttcatg accatggccg ccgacaccgg cgcgtcgatc
     4981 cggttcatcg gcgaccccgc ccagcacggc gccatcgccg ccggagggat gttccgggtg
     5041 ctgtgcgaac gccacccagc catcacgcct gag
//
LOCUS       DAOQOD010000206         5047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6873_length_5047_cov_11.160056, whole genome shotgun sequence.
ACCESSION   DAOQOD010000206 DAOQOD010000000
VERSION     DAOQOD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6873_length_5047_cov_11.160056"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLZ72_14370"
     CDS             <1..1464
                     /locus_tag="PLZ72_14370"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNA37529.1"
                     /translation="PAPWPLDESRLLASRATFGPTPAVVDRIRSIGPAAWLDEQLAAN
                     GATAEARLGSSFPSLTASNAVNAVQRNTDQNVLFRELDHAMLLRAVYSENQLYEVMCD
                     FWSNHFSIWRSAKWMTHLKTRDDGDVIRPHALGRFADMLKASARSPAMLVYLDNYSSN
                     AYSPSGVNENWGRELLELHTLGIIKGEQVYTEADMRGVAKVMSGRTINWTDGPTKYDY
                     RFEKYWHSRDAVSILGGQWSRPARTDGAQGEADGNSLLEFLARHPSTARHLAFKLCRR
                     FVADDPPMTLVDRLASTYLANDTAIAPVLRELFLSPELRASGGQKVKRPYEWLVSALR
                     ATKAEIDSDPQGHASRRLREVQWGLGQPINERTTPDGYPETAAEWVSTEGLLKRWEWG
                     ARLARNKLTSTTEARKVVIDPAALLPSPLPATTTDLLTALADQVFQFPLPAPHATSIL
                     AALGVSPSAAATTITSNANNLQAVLGLLCAHPNFQRR"
     gene            1479..2828
                     /locus_tag="PLZ72_14375"
     CDS             1479..2828
                     /locus_tag="PLZ72_14375"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNA37530.1"
                     /translation="MTLTHDTDTVPSGATRSAEGGPLSRRSFMGLVGAGTTALGVTGL
                     GARMAFATPASPSSGDVIVVVFMRGAWDGLSVVAPYQMPTYKALRPTIRIKEPAEFTD
                     PTGKAGLPLLDGGAVAPFPLSGTFAFNPSMTHLHQGAWADGHLAVVHAAGMPASESST
                     RSHFEAEQYWEKGTRSLNVTSGFLNRYLSGLSGLERLSAVGRGSTLQTSLQGAANAVS
                     MGSISGFGVTGFPNNTQARTALVGLYPHGPQLLEETGADTLDVVGLIAGMGADPGPQN
                     GATYGPDDLSNNLREVARLIRADVGLRAVAIDYGGWDTHSTMGRPEDAGSYMRQRTGV
                     VSQALQAFYTDLGAARDEVTLVTMSEFGRTIGENGSGGTDHGRGNVMFALGGKVRGGV
                     YGDFPATVEDGPEGDLTVMTDYRRVVSEILEVRGGATNPTAIFPTYTPQAPLGLTVS"
     gene            complement(2857..3333)
                     /locus_tag="PLZ72_14380"
     CDS             complement(2857..3333)
                     /locus_tag="PLZ72_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37531.1"
                     /translation="MDRLIVALVLAAIAVAVAAVLQRRRADPPTQPPSWEAPVQLDRG
                     DFDRPDAPWFVGVFSSATCSTCADVVAKAQILASDEVVVQDVEASRDAPLHRRYGIEA
                     VPIVVIADRDGVVRQSFVGPVSSTHLWAAVAELREPGSVPGSCDGHHDVGSGAEPT"
     gene            complement(3391..4869)
                     /locus_tag="PLZ72_14385"
     CDS             complement(3391..4869)
                     /locus_tag="PLZ72_14385"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HNA37532.1"
                     /translation="MRLGLVTVVALALVGLASVWSGRPDPEPAAVEQAPMPAVRTGEA
                     LPATWYCASGTASRALAHYVFLSNPTGDAATVRLTGYTAGGATPTQTVEVAPKGPLPL
                     DVGAVLGDPTASVLVESDVAGLVVDNQLSDEEAGDRAACARSSSDAWYFPALSITRDA
                     GARLTLFNPFPADAGVDIEIGLDTGTRVPASLAGLVVPAGTARVVDLGQAVQRRDQFT
                     AVVRSRSGRVVAAVAQTFDGSIGPEGLWLTNGVPGPQPRWAFAGGFTGTGVAERLVIQ
                     NPGDRRSRVLVQVTPYGGASDPPEPIEVAVEPRHYAVVDLSAETRIPGVGYHSIEVES
                     DRPVVVARTTTLSAGPDPATDAGIAVRPALGRGVALSTGTPVLARSWTVPSINAGADP
                     APVVAVHNPGEGIARVTLEVVAAGSSSALPGATDVEVAPGDSVVVPLGPAAAPEVAVV
                     VTSSEPVAVERITVYAPDVDLAFDPAVPGHGRNRPLVPLGSS"
BASE COUNT          753 a         1906 c         1736 g          652 t
ORIGIN      
        1 ccggctccgt ggccgctcga cgagtcccgg ctcctggcct cgcgcgcgac gttcggaccg
       61 accccggccg tggtcgaccg catccgcagc atcggccccg ccgcctggct cgacgagcag
      121 ctcgccgcga acggggcgac cgcagaggct cgcctgggct cctcgttccc gtcgctcacc
      181 gccagcaacg ccgtcaacgc ggtgcagcgc aacaccgatc agaacgtgct gttccgcgag
      241 ctcgaccacg ccatgctgct gcgggcggtg tactccgaga accagctcta cgaggtgatg
      301 tgcgacttct ggtccaacca cttctcgatc tggcgatcgg ccaagtggat gacgcacctc
      361 aagacccgcg acgacggcga cgtgatccga ccccacgctc tgggtcggtt cgccgacatg
      421 ctcaaggcct cggcgcgcag ccccgccatg ctggtgtacc tcgacaacta ctcgtccaac
      481 gcctactcgc cgtcaggggt caacgagaac tggggccgtg agctgctcga gctccacacc
      541 ctcgggatca tcaagggcga gcaggtctac accgaggccg acatgcgcgg cgtcgcaaag
      601 gtgatgtcgg gccgcacgat caactggacc gacggcccca ccaagtacga ctaccggttc
      661 gagaagtact ggcactcccg cgacgcggtg tcgatcctcg gcgggcagtg gagccgaccg
      721 gcgcggaccg acggcgccca gggcgaagcc gacggcaact ccctgctcga gttcctggcc
      781 cggcacccct cgacggcccg gcacctcgcg ttcaagctgt gtcgccgctt cgtggccgac
      841 gatcccccga tgacgctcgt cgaccgcctg gcgtcgacct acctggccaa cgacaccgcc
      901 atcgccccgg tgctgcggga gctgttcctg tccccggagc tccgggcctc tggcggccag
      961 aaggtgaagc gtccctacga gtggctcgtc tcggcgctcc gggcgacgaa agccgagatc
     1021 gactccgacc cccagggaca cgcgtccaga cggctgcgcg aggtccagtg ggggctcggg
     1081 cagccgatca acgaacgcac cacgcccgac ggctacccgg agaccgcggc ggagtgggtg
     1141 tccaccgagg gcctgctcaa gcgatgggag tggggcgccc gcctggcgcg caacaagctg
     1201 accagcacga ccgaggcccg gaaggtggtg atcgatcccg ccgccctgct cccctcgccg
     1261 ttgccggcca ccacgacgga cctgctcacc gcactggccg atcaggtgtt ccagttcccc
     1321 ctcccggcgc cgcacgcgac gtcgatcctc gctgcgctgg gggtctcacc ctccgcggcc
     1381 gccacgacga tcacctccaa cgccaacaac ctccaggccg tcctcggcct gctctgcgcc
     1441 catcccaact tccagcgccg ctgaccggag acgacgagat gaccctcacc cacgacaccg
     1501 acaccgtgcc ctccggtgcc acacgctccg ccgagggcgg accgctcagc cgacggtcct
     1561 tcatgggcct ggtcggcgcc gggaccaccg ccctgggcgt caccggcctg ggggcacgga
     1621 tggcgttcgc cacgcccgcc tccccgtcga gcggcgacgt gatcgtcgtc gttttcatgc
     1681 gcggcgcatg ggacggactc agcgtggtcg ccccctacca gatgcccacc tacaaggccc
     1741 tgcgcccgac gatccgcatc aaggagcccg ccgagttcac cgacccgaca gggaaggccg
     1801 ggctcccgct cctcgacggg ggtgccgtgg ccccgttccc gctgtcgggg acgttcgcct
     1861 tcaacccgtc gatgacccac ctgcaccagg gcgcatgggc cgacgggcac ctggcggtcg
     1921 tccacgccgc gggcatgccg gcgagcgagt cgtcgacccg cagccacttc gaagccgagc
     1981 agtactggga gaagggaacc aggtccctga acgtgaccag cggcttcctc aaccgctacc
     2041 tctccgggct gtccgggctc gagcggctga gcgccgtcgg acgcggctcg acgctgcaga
     2101 cgtcgttgca gggagcggca aacgccgtgt cgatgggctc gatctccggc ttcggcgtga
     2161 ccggcttccc gaacaacacc caggcccgga ccgcactcgt cggcctgtac ccgcacggac
     2221 cgcagctgct cgaggagacc ggagccgaca ccctcgacgt ggtcggtctg atcgcaggga
     2281 tgggcgctga ccccggcccg cagaacggcg cgacctacgg acccgacgac ctgtcgaaca
     2341 acctgcggga agtcgcccgt ctcatccgcg ccgacgtcgg cctgcgcgcc gtcgccatcg
     2401 actacggcgg gtgggacacc cacagcacca tgggccgacc cgaggacgcc gggtcgtaca
     2461 tgcgtcagcg gaccggcgtg gtctcccagg cgttgcaggc gttctacacc gacctgggcg
     2521 ccgcccggga cgaggtcacg ttggtcacga tgtcggagtt cggtcgcacg atcggcgaga
     2581 acggctccgg cggcaccgac cacggtcggg gcaacgtcat gttcgccctc ggcggcaagg
     2641 tcaggggtgg ggtgtacggg gacttccccg ccaccgtcga ggacggtccc gagggcgacc
     2701 tcaccgtcat gaccgactac cgacgcgtcg tgtcggagat cctcgaggtc cggggcgggg
     2761 ccaccaaccc gaccgcgatc ttcccgacct acacgccgca ggcgcccttg gggctgacgg
     2821 tcagctgact cctgagccgc ggaccagccg gggcagctac gtgggttccg cgcccgatcc
     2881 gacgtcgtgg tggccgtcgc agctgcccgg caccgacccc ggctcgcgca gttccgcaac
     2941 cgctgcccac aggtgcgtcg acgacaccgg ccccacgaac gactgtctca ccaccccgtc
     3001 ccggtcggcg atgacgacga tcgggacggc ctcgatgccg tagcgacggt gcagcggggc
     3061 gtcgcgggat gcctcgacgt cctgcaccac gacctcgtcg ctggcgagga tctgcgcctt
     3121 ggccaccacg tccgcgcacg tggagcaggt ggccgagctg aacactccca cgaaccacgg
     3181 cgcgtccggg cggtcgaagt cgcctcggtc gagctgcacc ggcgcctccc aggacggcgg
     3241 ctgggtgggc gggtcggccc ggcgacgttg caggaccgcc gccaccgcga ccgcgatcgc
     3301 agcgagcacc agggcgacga tcagtcgatc caccccgaca gtgtgccctg ggtccgcgtc
     3361 cggctcacag ctgggcgccc ccggacccgc tcagctcgac ccgagcggga ccagaggacg
     3421 gttccggccg tggccgggga ccgccggatc gaacgcgagg tcgacatcgg gtgcgtacac
     3481 ggtgatgcgc tccaccgcca ccggctcgct ggaggtgacg acgaccgcca cctcgggggc
     3541 ggcggccggg ccgagcggga ccacgacgct gtcgccggga gcgacctcca cgtcggtcgc
     3601 cccgggcagc gccgagctcg atccggccgc gacgacctcg agggtgaccc gggcgatccc
     3661 ctctccgggg ttgtggaccg cgaccaccgg cgccgggtcc gctccggcgt tgatcgacgg
     3721 gaccgtccaa ctccgggcga gaaccggggt gccggtggag agcgcgaccc ctcgcccgag
     3781 ggcgggacgg accgcgatcc cggcatcggt ggccggatcc gggccggcgc tgagcgtcgt
     3841 ggtgcgggcc acgaccaccg gtcggtcgga ctcgacctcg atcgagtggt agccgacacc
     3901 ggggatgcgg gtctcggccg acaggtccac caccgcgtag tggcgcggct cgacggcgac
     3961 ctcgatgggc tccggcgggt cggacgcacc gccgtagggt gtgacctgga ccaggaccct
     4021 ggaccgtcga tcccccgggt tctggatcac cagccgttcg gcgaccccgg ttccggtgaa
     4081 ccccccggcg aacgcccaac gtggctgcgg gccgggcacg ccgttggtca gccagagccc
     4141 ctccggtccg atcgatccgt cgaaggtctg ggccaccgcg gccacgaccc gtcccgacct
     4201 ggaacggacc acggcggtga actggtcgcg tcgctgcacg gcctgaccca ggtcgacgac
     4261 acgggcggtg cccgccggca ccaccagccc ggccagcgac gccggcacgc gcgtcccggt
     4321 gtcgagcccg atctcgatgt cgacaccggc gtccgcgggg aaggggttga acagggtcag
     4381 gcgggctccg gcgtcccggg tgatggacag cgccgggaag taccaggcgt cggagctcga
     4441 ccgggcgcag gcggcgcgat cacccgcctc ctcgtcggac agctggttgt cgacgaccag
     4501 tccggccacg tcggactcca ccagcaccga cgccgtcgga tcgccgagga cggctccgac
     4561 gtcgaggggc agagggccct tcggggccac ctcgaccgtc tgcgtcgggg tggcaccgcc
     4621 ggcggtgtag ccggtgaggc ggaccgtggc ggcgtccccg gtcgggttcg acaggaacac
     4681 gtagtgcgcg agggcgcgcg atgcggtgcc cgacgcgcag taccaggtgg cgggcagagc
     4741 ctctccggtg cgcaccgcgg gcatcggggc ctgttcgacc gcggccggtt ccggatcggg
     4801 acgtccggac cacacagagg ccaggccgac cagggcgaga gcgacgaccg tgacgagtcc
     4861 caggcgcatc agcttcacgt cgccccctca ccgtgatccg cgacggctcg ctcctccgcg
     4921 gccgctcccc ggtccgcggg gccggactcc ggcgccgcgt cggactcgac gaccacgacc
     4981 gccgggcccc gtcgcacccg gcgccgcggc cgcggggcag cgagccgtcg gcggcgaccc
     5041 accatcg
//
LOCUS       DAOQOD010000207         5029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_6914_length_5029_cov_11.190189, whole genome shotgun sequence.
ACCESSION   DAOQOD010000207 DAOQOD010000000
VERSION     DAOQOD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6914_length_5029_cov_11.190189"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..650)
                     /gene="ribH"
                     /locus_tag="PLZ72_14390"
     CDS             complement(147..650)
                     /gene="ribH"
                     /locus_tag="PLZ72_14390"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR00114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNA37533.1"
                     /translation="MARNFSTPDVTGASVDGTGRRVGIVCARWNRTVTDRLLRGATDT
                     LRTCGLRDDDIDVAWVPGAFELPLAAKVMAGTGRYDAVIAIGCVIRGDTAHFEYVAGP
                     AAAGIMDAQLAVEVPIVLAVLTVEDRRQALVRSVVDGDVTGDNKGSEAAETALEMAAV
                     LDRLRVG"
     gene            complement(672..1922)
                     /locus_tag="PLZ72_14395"
     CDS             complement(672..1922)
                     /locus_tag="PLZ72_14395"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNA37534.1"
                     /translation="MTREPLSEQGSESSVFASVEDAVAAIARGEIIVVVDDEDRENEG
                     DLIMAAGAASAETITFFVRHTSGVICAPLTGERLDELEIPLMVRDNTESHRTAFTYSV
                     DYVHGTSTGISAADRAATIQALIDPATRPADLARPGHIFPLRYSEGGVLKRAGHTEAA
                     VDLARLAGMDPAGVLCEIVNDDGTMARVPDLIEFCREHGLLMITIAQLIKYRRQNEKL
                     VRRVAEARIPTPWGDFTCYVYESVLDGEQHVAMVRGAVQGEEDVLVRVHSECLTGDVF
                     HSLRCDCGVQLDAAMERIAEEGLGVLVYLRGHEGRGIGIGHKIRAYSLQEEGHDTVEA
                     NVELGLPVDSREYGIGAQMLNDLGITTMRLMTNNPAKYGGLEGFGLEITERVPVLSAP
                     NPENIGYLRTKREKMGHLLENLDD"
     gene            complement(1919..2518)
                     /locus_tag="PLZ72_14400"
     CDS             complement(1919..2518)
                     /locus_tag="PLZ72_14400"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNA37535.1"
                     /translation="MFTGIVEELGSVDSLDGPRLRIRGSTVLDDVTMGASTAVNGVCL
                     TVVAWGDDWWEADVSDETFARSALGSLAPGDPVNLERPVRLQDRLGGHLVQGHVDAVG
                     QVVAPAPDLAVQVPSDLVRYLVEKGSVTVDGVSLTVVEVRNDVFTVALIPHTSEVTTL
                     GRRAVGDPVNIEVDVMAKYAERLLGGVLDRTRSESEDPS"
     gene            complement(2518..3627)
                     /gene="ribD"
                     /locus_tag="PLZ72_14405"
     CDS             complement(2518..3627)
                     /gene="ribD"
                     /locus_tag="PLZ72_14405"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNA37536.1"
                     /translation="MNAPTPTDGPRPPGPHDAVDRECMTRALAAAATVRCATSPNPWV
                     GAVVRTTDGRMFHGATEAPGGAHAEIVALRQAGDQARGATLYVTLEPCAHTGRTGPCT
                     RAIIDAGISRVVVGIEDPDPQVTGRGLDQLRAAGIDVTLGVQADHVAGQLTAYLTHRR
                     TGRPYVVLKLAATLDGGTAAPNGTSQWITCPEARADGHRLRAESDAILVGAGTIRRDD
                     PSLTVRDYRPPVMPAGGSVDPLRVVLGAVDDHARVQPCYSTSGDLGDVLDDLGSKGIL
                     QLLVEGGAKVAGEFHRAGLVDRYVIYTAPALFGGDDARGLFGGNGAWDISEVRRGRFV
                     AVERVGTDLRLEMVPDHTGAGTDGSGVEPGSAGGS"
     gene            complement(3781..4287)
                     /locus_tag="PLZ72_14410"
     CDS             complement(3781..4287)
                     /locus_tag="PLZ72_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNA37537.1"
                     /translation="MTDSSAPTRHHQAKVLTVSDGVIAGVREDRSGAALVSLLEERGF
                     EVIEHRVVSDGIEGVSNALSYMAYGFNGLVVTTGGTGFGVRDLTPEATKRILDRTAPG
                     LAEAMRAVNPLGRLSRGIAGIRGSALLLNTPGSTSGAVEMLDAVLDVVPHALDLLGGA
                     SGGHPTSA"
     gene            complement(4284..>5029)
                     /locus_tag="PLZ72_14415"
     CDS             complement(4284..>5029)
                     /locus_tag="PLZ72_14415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNA37538.1"
                     /translation="VADLGHDDALAALETMDLAPRVHRRADGYTQDLASQWVAGLVDA
                     GPGDRVADLCAAPGGKATAMAGVGAHVVAADLSPARAGLVVANARTTAPERPGSGRLD
                     VVVADATAPPLAPGRFDRVLIDAPCSGLGVLRRRPDARWRIGPDAPERLGALATRMVD
                     AAVPLLADGGTLVFSVCTLTAAESLAVDDHVSARHPALEPLPAPDEPWRPWGRGAILL
                     PQAADTDGMCLFRYHRVGPGPHGRYDPGP"
BASE COUNT          673 a         1774 c         1885 g          697 t
ORIGIN      
        1 tctcgcgccg gcatcgatcg ccggggccga cgccgcggtg aggatcccga tggcccccgg
       61 ggtcgactcg ctcaacgtgg cgacatcgct ggcggtggtc gctgcgttcg cggccgcgcg
      121 ccggcagtgg cgctgacgtc gtccgctcag ccgacgcgca gacgatcgag cacggcagcc
      181 atctcgagcg ccgtctcggc ggcctcggag cccttgttgt cgccggtgac gtcgccgtcg
      241 accaccgagc gcaccagcgc ctgacgtcgg tcctcgacgg tgagcacggc cagcacgatg
      301 ggaacctcga cggcgagctg ggcatccatg atgcccgccg cagccgggcc ggcgacgtac
      361 tcgaagtgcg cggtgtcgcc gcggatgacg cacccgatgg cgatcaccgc gtcgtagcgg
      421 ccggtgccgg ccatgacctt ggccgccagc gggagctcga acgcccccgg cacccacgcg
      481 acgtcgatgt cgtcgtcgcg gagcccgcag gtccgcaggg tgtcggtcgc accgcggagc
      541 agtcggtcgg tgaccgtgcg gttccaacgg gcgcagacga tgccgacgcg gcggccggtg
      601 ccatcgacgc tggccccggt gacgtcgggg gtggagaagt tgcgggccat gcggatcctc
      661 cggcggatcg gtcagtcgtc caggttctcg agcaggtggc ccatcttctc ccgcttggtg
      721 cgcaggtagc cgatgttctc ggggttcggg gccgacagga ccggcacccg ctcggtgatc
      781 tccagcccga agccctcgag cccgccgtac ttggccgggt tgttggtcat caggcgcatg
      841 gtggtgatgc ccaggtcgtt gagcatctgc gcgccgatcc cgtactcgcg ggagtcgacc
      901 ggcagtccca gctcgacgtt ggcctcgacg gtgtcgtgac cctcctcctg gaggctgtag
      961 gcgcggatct tgtggccgat gccgatcccg cggccctcgt ggccgcgcag gtagaccagg
     1021 acgcccaggc cctcctccgc gatccgctcc atggcggcgt cgagctgcac tccgcagtcg
     1081 cagcgcagcg agtggaacac gtcgccggtc aggcactcgg agtggacccg caccagcacg
     1141 tcctcctcgc cctggaccgc accgcggacc atggcgacgt gctgctcgcc gtcgaggacg
     1201 gactcgtaga cgtagcaggt gaagtcgccc cacggggtgg ggatccgggc ctcggccacc
     1261 ctccgcacga gcttctcgtt ctgacggcgg tacttgatca gctgggcgat cgtgatcatg
     1321 agcagcccgt gctcccggca gaactcgatc aggtccggca cccgggccat ggtcccgtcg
     1381 tcgttgacga tctcgcagag gacgcccgcg gggtccatac cggcgaggcg ggccaggtcg
     1441 acggcggcct cggtgtggcc cgcccgcttg agcaccccgc cctcggagta ccgcagcggg
     1501 aagatgtggc cggggcgggc caggtccgcc gggcgggtgg cggggtcgat gagcgcctgg
     1561 atggtcgcgg cccggtccgc ggccgagatg cccgtcgagg tgccgtggac gtagtcgacc
     1621 gagtaggtga aggccgtgcg gtgcgactcg gtgttgtcgc gcaccatcag cgggatctcg
     1681 agctcgtcga gtcgttcgcc ggtcagcgga gcgcagatca ccccggaggt gtggcggacg
     1741 aagaaggtga tggtctcggc gctggccgcg ccggcggcca tgatcaggtc gccctcgttc
     1801 tcgcggtcct cgtcgtcgac gaccacgatg atctcgcctc gggcgatggc cgcgacggcg
     1861 tcctcgaccg aggcgaacac cgagctctcg ctcccctgct cggacagcgg ttcacgggtc
     1921 atgacgggtc ctccgattcg gaacgggtgc ggtcgagcac gccgccgagc aggcgctcgg
     1981 cgtacttggc catgacgtcg acctcgatgt tgaccgggtc gccgacggca cgtcgaccga
     2041 gcgtggtcac ctcggacgtg tgggggatca acgcaacggt gaacacgtcg ttgcggacct
     2101 cgacgacggt caggctgacg ccgtcgacgg tgacggaccc cttctcgacc aggtagcgca
     2161 ccaggtccga cgggacctgc accgcgaggt cgggcgcggg tgccaccacc tgcccgaccg
     2221 catcgacgtg accctggacc aggtgcccgc ccaggcggtc ctgtagacgt accggtcgtt
     2281 cgaggttgac cgggtctccg ggggcgagcg agccgagggc gctgcgggcg aaggtctcgt
     2341 cgctgacgtc ggcctcccac cagtcgtcac cccaggcgac cacggtgagg cacacgccgt
     2401 tgaccgcggt cgacgcgccc atggtgacgt cgtcgagcac ggtcgagccc cggatccgca
     2461 gcctcggccc gtcgagggag tcgaccgatc ccagctcctc cacgatcccg gtgaacatca
     2521 cgaacctcct gcggagccgg gctcgacgcc ggatccgtcg gtgcccgcgc ccgtgtggtc
     2581 agggaccatc tccaggcgca gatcggtgcc gacccgctcg acggcgacga agcggccgcg
     2641 gcggacctcg gagatgtccc acgccccgtt gccgccgaac agaccgcggg cgtcgtcgcc
     2701 gccgaacagc gcgggggcgg tgtagatcac gtagcggtcg accagcccgg cccggtggaa
     2761 ctcccctgcc accttggccc cgccctcgac caggagctgc aggatgccct tggagccgag
     2821 gtcgtccagc acgtcgccga ggtcgccgga ggtcgagtag cagggctgga cccgggcgtg
     2881 gtcgtccacc gcgccgagca ccacgcgcag cgggtcgacg gatccgccgg ccggcatcac
     2941 cggaggccgg tagtcgcgca cggtgagcga cgggtcgtcg cgacggatcg tgccggcgcc
     3001 gaccaggatg gcgtcggact cggcccgcag gcggtgcccg tcggcgcggg cctcgggaca
     3061 ggtgatccac tggctggttc cgttgggagc cgcggtcccg ccgtcgagcg tggcggccag
     3121 cttgagcacc acgtacgggc gaccggttcg acggtgggtg aggtaggcgg tgagctggcc
     3181 ggcgacgtgg tcggcctgaa ccccgagggt cacgtcgatg cccgcggcgc ggagctggtc
     3241 caggccccga cccgtgacct gggggtcggg gtcctcgatg ccgacgacga cccgtgagat
     3301 cccggcgtcg atgatcgccc gggtgcacgg cccggtgcgg ccggtgtgag cgcacggttc
     3361 gagcgtgacg tagagcgtcg ctcctcgggc ctggtcgccg gcctgccgga gggcgacgat
     3421 ctcggcgtgc gcccctccgg gcgcctcggt ggccccgtgg aacatgcgcc cgtccgttgt
     3481 ccgcaccacg gccccgaccc acgggttcgg cgacgtcgcg caccgcacgg tggcggctgc
     3541 cgcgagcgcc cgggtcatgc actcgcggtc gaccgcgtcg tggggcccgg gtgggcgggg
     3601 accgtcggtg ggcgtgggag cgttcatcgg gatcgggccg gaccgtccgg tgcgcctgtg
     3661 catcgacggg ccgacggtgg agccagatgg taccgcccgg gccctgcctg gtcgaatctt
     3721 gcccggtcgt tcagattacg accgggcacc tccgatcggt ccgacgccgc cgggacgggc
     3781 tcacgccgag gtcgggtgac cgccggatgc cccgccgagg aggtcgagag cgtggggaac
     3841 gacgtcgagc accgcgtcga gcatctccac cgcccccgag gtggacccgg gggtgttgag
     3901 cagcagcgcg gagccacgga tgccggcgat gccccgcgac agccgtccga gcgggttcac
     3961 cgcccgcatc gcctcggcca gacccggcgc ggtccggtcg aggatccgct tggtggcctc
     4021 gggagtcagg tcgcgcacgc cgaacccggt gccaccggtg gtgaccacca gcccgttgaa
     4081 cccgtaggcc atgtaggaca acgcgttgga caccccctcg atgccgtcgg agaccacccg
     4141 gtgctcgatc acctcgaagc cccgctcctc gagcagcgac accagcgctg cgccgctgcg
     4201 gtcctcgcgc acgccggcga tcaccccgtc ggacacggtc aggaccttgg cctggtggtg
     4261 acgggtcgga gcggagctgt cggtcatggg cccggatcgt accgaccgtg ggggccgggc
     4321 ccgacccggt ggtagcggaa caggcacatc ccgtcggtgt cggcggcctg gggcagcagg
     4381 atcgcccccc tgccccacgg tcgccacggc tcgtcggggg ccggcagcgg ttcgagagcg
     4441 gggtgccggg ccgagacgtg gtcgtcgacg gcgaggctct cggccgcggt gagggtgcac
     4501 acgctgaaga ccagggtgcc gccgtcggcc agcaacggca ccgccgcgtc gaccatccgg
     4561 gtggcgaggg cgccgaggcg ctcgggagca tccggaccga tccgccaccg ggcgtccggt
     4621 cgtcggcgca gcacgcccag cccgctgcac ggggcgtcga tgaggacgcg gtcgaaccga
     4681 ccgggggcga gcggaggggc ggtggcgtcg gccacgacga cgtcgagccg gccggacccg
     4741 ggccgttcgg gagcggtcgt ccgggcgttg gcgacgacta gcccggcccg ggccgggctc
     4801 aggtccgcgg cgaccacgtg cgcaccgacg ccggccatgg ccgtcgcctt cccgccgggg
     4861 gcggcgcaca ggtcggcgac ccgatcaccg ggaccggcgt cgacgagccc ggcgacccac
     4921 tgcgaggcca ggtcctgggt gtagccgtcg gctcgccggt ggacccgggg agcgaggtcc
     4981 atcgtctcga gcgcggccaa cgcgtcgtcg tggcccagat cggccacca
//
LOCUS       DAOQOD010000208         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7038_length_4982_cov_8.800081, whole genome shotgun sequence.
ACCESSION   DAOQOD010000208 DAOQOD010000000
VERSION     DAOQOD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_4982_cov_8.800081"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2773)
                     /gene="valS"
                     /locus_tag="PLZ72_14420"
     CDS             complement(74..2773)
                     /gene="valS"
                     /locus_tag="PLZ72_14420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNA37539.1"
                     /translation="MPASPPPSAADPSAGTPTAGSRPPVRRGTRAVPDKPGVDGLEAK
                     WSEQWEADGTYSFDRTADRDRVFSIDTPPPTVSGSLHVGHVFSYTHTDVVARFQRMRG
                     KAVFYPMGWDDNGLPTERRVENYYGVVCDPSVPVGMPELADPPPTKRQDFTRVSRPDF
                     IELCERLLVVDEEAFEALFRTLGLSVDWSYTYATIDEASRRTSQLAFLHDLARGDAYS
                     QEAPSLWDVTFQTAVAQAELEDRDRPGAYHQLAFALTGGATTADGSADVVIDTTRPEL
                     VVSCVALVAHPDDERYQPLFGRTVTTPVFGVEVPIVAHPLAEPDKGTGIAMICTFGDL
                     TDVIWWRELDLPTRAVIGRDGRFAAEAPDWLDTADAQERYGRIARKAAGGAREQMVQM
                     LADEGRLLGDIRPIQHPVKFYEKGDKPLEIVTSRQWYIRNGGRSEDRRRELIDRGGEL
                     DWVPGYMRHRFDNWVGGLAGDWLISRQRFFGVPFPLWYRLDDEGNPDHDHPIVPDEAS
                     LPVDPLAEAAPGYSEDQRGVPGGFIGDPDVMDTWATSSLTPQIGCRWVDDPDLFARTF
                     PMDLRPQAHDIIRTWLFSTVLRSDLEFDSLPWAHTALSGWILDPDRKKMSKSKGNVVT
                     PLGLLEQYGSDGVRYWAASARPGTDTAFDEAQMKVGRRLAIKLLNASRFALSFGEVPA
                     GTPVTEALDRAMLAELADLVDDATTALEAFDYARALERTETFFWRFCDDYLELVKGRA
                     YGEDDGSGPAGGGGGGTAPSAATASARAALAEALSVLHRLLAPVLVYTCEEAWSWWME
                     GSVHTAEWPAAAPLRARAVDGRRDALAVAASVLSDLRGAKSAAKVSMRTPIERAVVTD
                     TAERIALLRTVAVDVADAGKVTDLVLVEGDTFAVEVTLSDPDA"
     gene            2868..3734
                     /locus_tag="PLZ72_14425"
     CDS             2868..3734
                     /locus_tag="PLZ72_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA37540.1"
                     /translation="MPSPSLDGVAIVTGAGGGIGAAVARSLAAAGTSLVLADRDRSAL
                     EAVAASLPPSVTVITGVGDVGDPTHHAALVTAAEDLGGVTVSVQNAGVALTGLSWEVP
                     LEQWELQVRTNYWGVVHGVRAALPPMVRRGLGHVLAVASGAGLVATPGLAPYVSSKHA
                     VVGLMESVRHELARAAPGVRASVVCPGNIATPINANSLAVADAAGAADVAACEGSAGD
                     GSTAAVARQIDETVRRGVDSGAEPQTVADAIVEAVSTGRFWVLPQPEVALGALDRVQR
                     IVDGRPPADLLI"
     gene            complement(3805..>4982)
                     /locus_tag="PLZ72_14430"
     CDS             complement(3805..>4982)
                     /locus_tag="PLZ72_14430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNA37541.1"
                     /translation="TVLAPMAHRSVDAHLAGADARADLDALRRTVTIRDRFDDAVAAE
                     GAEAAVLHLLVSAALEVTPDADVSLLLSLPDEPRVAWSVRSAGDRLLPAEPVPGRPSC
                     VALATGRTAVAPSSTEMGACGHLRESADGVHDLGDGHDPGDGHDSDDGGEPGDGDGVG
                     SAAEEPSWEPTEVAALCLPLVIGERNLGVLCVTTAPGELPDRDRQATLEHVVHVGSAR
                     LESLRQRRGPSTPGPTDPVTGLPTEVALRARAGELTRSHQPWCLAVLRVDGADEFRNL
                     HGPHTWDDAMRLIADTTSTTLRPDDVVVRLDGERVAAVLSGCSTAQAAAALERARESL
                     VLAHTMAGIEHFTCSVGLVDGTRTTSIDDGIRLADAAGHAALLQGGNRVVVADASII"
BASE COUNT          670 a         1712 c         1928 g          672 t
ORIGIN      
        1 tgttcgttgt ttcacaacgt cgacgttgtg aaacaacgaa cagaacgggg tggaggggtg
       61 taagggaggg ggatcaggcg tcggggtcgg acaaggtgac ctcgacggcg aaggtgtcgc
      121 cctcgaccag gacgaggtcg gtcaccttgc cggcgtccgc gacgtcgacg gcgacggtgc
      181 gcagcagcgc gatccgctcg gcggtgtcgg tgacgacggc gcgttcgatc ggggtccgca
      241 tcgacacctt ggccgccgac ttggcgcccc gcagatccga caggaccgac gcggcgaccg
      301 ccagggcgtc gcgtcgaccg tcgacggccc gggctcgcag cggtgcggcg gcgggccact
      361 cggcggtatg caccgagccc tccatccacc acgaccaggc ctcctcgcag gtgtagacga
      421 gcacgggcgc cagcagccgg tgcagcaccg acagcgcctc ggccaacgcg gcccgggccg
      481 aggccgtcgc cgcgctcggc gctgtcccac cgccaccgcc accggccggt ccggatccgt
      541 cgtcctcgcc gtaggcgcgg cccttcacca gttcgaggta gtcgtcgcag aagcgccaga
      601 agaacgtctc ggtgcgctcc aacgcccggg cgtagtcgaa ggcctccagt gcggtggtgg
      661 cgtcgtcgac caggtcggcg agctcggcga gcatcgcccg gtcgagcgcc tcggtgaccg
      721 gggtgccggc ggggacctcg ccgaaggaca aggcgaagcg gctggcgttg agcagcttga
      781 tcgccaggcg gcggccgacc ttcatctgcg cctcgtcgaa ggcggtgtcg gtgccgggac
      841 gggcgctggc cgcccagtag cgcacgccgt cggagccgta ctgctccagc agcccgagcg
      901 gcgtgaccac gttgcccttg gacttggaca tcttcttgcg gtccggatcc aggatccacc
      961 ccgacagggc ggtgtgcgcc cacggcagcg agtcgaactc caggtcggag cgcagcacgg
     1021 tcgagaacag ccaggtgcgg atgatgtcgt gggcctgggg ccgcaggtcc atcgggaacg
     1081 tgcgggcgaa caggtcgggg tcgtcgaccc agcggcagcc gatctgcggc gtcagcgacg
     1141 acgtcgccca ggtgtccatg acgtcggggt cgccgatgaa cccgccggga actccgcgct
     1201 ggtcctcgga gtagccgggg gcggcctcgg ccagcgggtc gaccggcagc gacgcctcgt
     1261 cggggacgat cgggtggtcg tggtcggggt tgccctcgtc gtcgagccgg taccacagcg
     1321 ggaacggcac gccgaagaac cgctggcggg agatcagcca gtcaccggcc aggccgccga
     1381 cccagttgtc gaagcggtgc cgcatgtagc cgggcaccca gtcgagctcg ccgccccggt
     1441 cgatcagctc gcggcgacgg tcctcggagc ggccgccgtt gcggatgtac cactggcggc
     1501 tggtgacgat ctcgagcggc ttgtcgccct tctcgtagaa cttgaccggg tgctggatcg
     1561 ggcggatgtc gccgagcagc cggccttcgt cggcgagcat ctgcaccatc tgctcccgcg
     1621 ccccgcccgc ggccttgcgg gcgatgcggc cgtagcgctc ctgcgcgtca gcggtgtcga
     1681 gccagtcggg ggcctcggcg gcgaaccgtc cgtcgcggcc gatgacggcc cgggtcggca
     1741 ggtcgagctc ccgccaccag atcacgtcgg tgaggtcgcc gaacgtgcag atcatggcga
     1801 tgccggtgcc cttgtcgggc tcggccagtg ggtgggcgac gatcggcacc tcgacgccga
     1861 acaccggggt ggtgaccgtg cggccgaaca gcggctggta gcgctcgtcg tcggggtggg
     1921 cgaccagcgc cacgcacgag acgaccagct cgggccgggt ggtgtcgatg acgacgtcgg
     1981 cgctgccgtc ggcggtcgtc gccccgccgg tgagggcgaa ggcgagctgg tggtaggcgc
     2041 cggggcggtc gcggtcctcg agctcggcct gcgcgaccgc ggtctggaac gtgacgtccc
     2101 acagcgacgg cgcctcctgg gagtaggcgt cgccgcgggc gaggtcgtgc aggaaggcga
     2161 gctgcgaggt ccggcggctg gcctcgtcga tggtggcgta ggtgtaggac cagtcgaccg
     2221 acaacccgag ggtgcggaac agcgcctcga acgcctcttc gtcgacgacg agcaggcgtt
     2281 cgcacaactc gatgaagtcg ggccggctga cgcgggtgaa gtcctggcgc ttggtcggcg
     2341 gcggatcggc cagttcgggc atgccgacgg ggaccgacgg gtcgcacacg acgccgtagt
     2401 agttctcgac ccgccgctcg gtgggaaggc cgttgtcgtc ccagcccatc gggtagaaga
     2461 ccgccttgcc ccgcatgcgc tggaaccggg cgacgacgtc ggtgtgggtg tagctgaaga
     2521 cgtggccgac gtgcagcgat cccgacaccg tcggcggcgg cgtgtcgatc gagaacaccc
     2581 ggtcgcggtc cgcagtgcgg tcgaaggagt aggtgccgtc ggcctcccac tgttcggacc
     2641 acttggcctc gaggccgtcg acgcccggct tgtcggggac cgcccgggtg ccacgacgga
     2701 ccgggggtcg cgagccggcg gtgggggtcc cggcggaggg gtcggcggcg gagggcgggg
     2761 gagaggcggg catacggcgg ctcacgctac cggccctcgg cactagcgtc cgaaggcgat
     2821 ccggggaccg gtacgggtcc gcggaggccg aagggggagg tcgccacatg cccagtccgt
     2881 cgctcgacgg cgtcgccatc gtgaccggtg ccggcggagg catcggcgca gcggtggcgc
     2941 ggtcgctggc cgccgccggc acgtccttgg tgctcgccga ccgcgaccgc tccgcgctgg
     3001 aggccgtcgc cgcctcgctg cccccgtcgg tcaccgtcat caccggcgtg ggcgacgtgg
     3061 gcgacccgac ccatcacgcc gcgctggtca ccgcggccga ggacctgggc ggggtgaccg
     3121 tgtcggtgca gaacgcgggg gtcgcgctga ccggcctcag ctgggaggtc ccgctcgagc
     3181 agtgggagct ccaggtgcgc accaactact ggggggtggt gcacggcgtg cgcgccgcgc
     3241 tgccgccgat ggtgcgtcgc ggcctgggac acgtgctggc ggtggcgtcg ggggcggggt
     3301 tggtggccac gccggggctg gcgccgtacg tgtcgtcgaa gcacgcggtg gtcgggctga
     3361 tggagtcggt gcgccacgag ctggcccggg ccgcgcccgg ggtgcgggcg tcggtggtgt
     3421 gcccgggcaa catcgccacg ccgatcaacg ccaactcgct cgccgtcgcc gacgccgccg
     3481 gagcggccga cgtcgccgcc tgcgagggga gcgcgggaga cgggtcgacc gcagcggtgg
     3541 cccgtcagat cgacgagacg gtccgccggg gggtggactc cggcgccgag ccgcagacgg
     3601 tggccgacgc gatcgtcgag gcggtgagca ccggccggtt ctgggtgctg ccccagcccg
     3661 aggtcgcgct cggcgccctc gaccgggtgc agcgcatcgt cgacggccgc ccccccgccg
     3721 acctcctgat ataggggtcc tcactcacaa cccttgtcac aatcccgacc tcagaaatcc
     3781 ggcccggccg gcccggccgt caggtcagat gatcgacgcg tcggcgacca cgacccggtt
     3841 gccaccctgc agcagcgcgg cgtggccggc ggcgtcggcc aggcggatcc cgtcgtcgat
     3901 cgaggtcgtc cgggtgccgt cgaccaaccc gaccgagcag gtgaagtgct cgatgcccgc
     3961 catggtgtgg gccagcacca gtgactcccg ggctcgttcc agcgcggcgg cggcttgggc
     4021 ggtgctgcag cccgacagca cggcggccac ccgctcgccg tcgaggcgca ccacgacgtc
     4081 gtcggggcgg agggtggtcg aggtggtgtc ggcgatcagg cgcatggcgt catcccatgt
     4141 gtggggaccg tggaggttgc ggaactcgtc ggctccgtcg acgcgcagga ccgcaaggca
     4201 ccacggctgg tgcgagcggg tgagctcgcc ggcccgggcc cgcagggcga cctcggtggg
     4261 aaggccggtg acggggtcgg tgggaccggg ggtggacggg ccgcgacgct gacgcagcga
     4321 ttcgagccga gcgctgccca cgtggaccac gtgctcgagg gtggcctgac ggtcgcggtc
     4381 ggggagctcg ccgggagcag tggtcacgca cagcaccccc aggttccgct cgccgatcac
     4441 caacggcagg cagagcgcgg cgacctcggt cggttcccac gacggctcct ccgccgctga
     4501 cccgacgccg tccccgtcgc cgggctcacc cccgtcatcg gagtcgtgcc catcaccggg
     4561 atcgtgcccg tcgccgagat cgtgcacgcc gtctgcggat tcgcggagat ggccgcatgc
     4621 gcccatctcc gtcgacgacg gcgccaccgc ggtgcgaccg gtggccaacg ccacgcagct
     4681 cggccgcccc gggaccggtt cggccggcag cagccggtcc ccggccgaac ggaccgacca
     4741 cgccactcgg ggttcgtcgg gcaacgacag gagcagcgac acgtccgcgt cgggggtcac
     4801 ctcgagcgcg gcgctcacca gcaggtgaag cacggcggcc tctgctccct cggcggcgac
     4861 ggcgtcgtcg aagcggtccc tgatcgtcac cgtccggcgg agcgcgtcga ggtcggctct
     4921 ggcatccgcg ccggcgaggt gcgcgtcgac gctgcggtgt gccatcggcg cgagcacggt
     4981 cc
//
LOCUS       DAOQOD010000209         4972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7068_length_4972_cov_6.904617, whole genome shotgun sequence.
ACCESSION   DAOQOD010000209 DAOQOD010000000
VERSION     DAOQOD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_4972_cov_6.904617"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLZ72_14435"
     CDS             complement(<1..940)
                     /locus_tag="PLZ72_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNA37542.1"
                     /translation="MDFIQYLRDRISELRSERDAAHTAVTDVLAAPTAESRDLNETEA
                     AAFAEARDHLAEVDTQIADFEARLDELVAIEQRHAEADAARPTLPAGEGVIGAEARTY
                     RPDRNHSFLADLYATSFRTGDVAGAEARMGRHNQEVAVEHRDIGVAAMAGAVPPQYLV
                     DKFAPVARAGRPFLNSLNAMDLPPEGVSFTVPRGTTGSAGAMTAEAAAFNEQDMANTD
                     LTSTVNLVTAAQDISRTLFMRGGPVVDQVIFPDLFAASEVALNASAVNGNGTAPQHRG
                     ILQVAGINAVTYTDASPTVAEAWPKLADAIQRINSAR"
     gene            complement(1073..1789)
                     /locus_tag="PLZ72_14440"
     CDS             complement(1073..1789)
                     /locus_tag="PLZ72_14440"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HNA37543.1"
                     /translation="MIRDRLTLPAEVRARLDLTEGDLIAAGIACRSRGALVEARRSPE
                     QPVELRSDGDTVGLGGYAAVFDVGYDVAGGPPWGWTETIAPGAFTKALREADDVRFLI
                     NHDGLPLARTKPGTLTLAQDDVGLRVDVPELDLVNPDAVKLRSTLERGDVDQMSHAFI
                     ALRQEWNDDYTERRILEVRLFDVSAVTYPANDVTVIALRAAAGIDPDTGNGDPEPEQI
                     AERTGMPLSLAAAQAAALGV"
     gene            complement(1786..2970)
                     /locus_tag="PLZ72_14445"
     CDS             complement(1786..2970)
                     /locus_tag="PLZ72_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNA37544.1"
                     /translation="MAGTLMGLIRPNRERRSLTDPVAEMWAARRGSFGETVTPERALQ
                     LAAVWSCYRILAGIGSTLPVDQYRGDVEVARDPLLDEPFPGQPLPDWLHRLWVSLLST
                     GNAYGWCSGPPGVIAGRTPRQVELLPAGAVKWEKDRSKRWTAKVNGTAEKLWPHGQLW
                     HLPLFTVAGRPEGLSPIGQAAATIHSGLSAQEFGNRWFDDGGHPSAILYSEDPNLDAT
                     GARKIKAAFQEATRGTREPAVLGSGLRYERIQLAPNESQFLDSMQWSSAQIAAAIGVP
                     AEWIGSAVSGQNVTYGNREQLWSDWTARDFAPYLVRIETGIGQLLPRGERVRHNLDAI
                     LRADLAARYTSYEVAARIFDATGVPLLTNDEMRRLENLPPLDGDTTFTRRTTKPTTTE
                     AP"
     gene            complement(2955..3086)
                     /locus_tag="PLZ72_14450"
     CDS             complement(2955..3086)
                     /locus_tag="PLZ72_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37545.1"
                     /translation="MLSTLLELVGLALVVVAGFIFSPVVGFVAAGAALVLVGWWLER"
     gene            complement(3097..4602)
                     /locus_tag="PLZ72_14455"
     CDS             complement(3097..4602)
                     /locus_tag="PLZ72_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37546.1"
                     /translation="MRTSWPPDVDPAPGLLVVPPDVESLDEAHAAIEMWEHYSAKTLD
                     PSQRLVVEVLMATRSDGRWAAATTGRAMARQNGKGDEIEVVELWGLVQRGERILHTVH
                     DAVLLTTQAQQRLLSVLESSPDLRSRITRIWRGTGQQMIELSNDATIWYRTRSGGGGR
                     GVDDVDRVVVDEAQHALDEHIDAISPTLLANPNGQLNAMGTSGLPGRSAWWWSQRRRA
                     LGDDPGRFGWVEHTAEVVHLDDTGNVVQEPVDVDDRSLWRATNPSVLAGRGQGMEFLE
                     EQLRRLGPAGFAREHLCVWAPPPVVDTDALIPFTEWTAGGRPLTAMTPVASGQPVLCW
                     SVSFDGKSSSIGMGWGSIADASVMVLDTGLAIRWLPGRLVELVAEWDPVAVGCNNAGP
                     TAAQVPVVLDALKAAGVEDRLHLFGLAEWKAACGGFLAAVREQQLVHLDGQGPLDTAV
                     AGVRERRLGDGFAWDGRASDVDLSPLEAVTGARALLPTERAAATVAPTYAF"
BASE COUNT          667 a         1603 c         1936 g          766 t
ORIGIN      
        1 accgcgccga gttgatccgc tggatggcgt ccgcgagctt cggccacgcc tcggcgaccg
       61 tcggcgacgc gtcggtgtag gtgacggcgt tgatgccggc gacctgcagg atgccgcggt
      121 gctgcggggc ggtgccgttg ccgttgacgg ccgacgcgtt gagcgccacc tccgacgcgg
      181 cgaacaggtc cgggaagatg acctggtcga cgacgggtcc gccgcgcatg aacagggtgc
      241 gggagatgtc ctgcgctgcg gtcaccaggt tcaccgtcga ggtgaggtcg gtgttcgcca
      301 tgtcctgctc gttgaacgcg gcggcctctg cggtcatggc gccggcggag ccggtggtgc
      361 cacgcgggac ggtgaagctc acgccctccg gggggaggtc catggcgttc agcgagttca
      421 ggaacgggcg cccggctcgg gcgacgggcg cgaacttgtc gacgaggtac tgcgggggga
      481 ccgctccggc catggccgcc acgccgatgt cgcggtgctc gacggcgacc tcctggttgt
      541 ggcggcccat gcgggcctcg gcgccggcga cgtcgccggt gcggaagctg gtcgcgtaca
      601 ggtcggcgag gaagctgtgg ttccggtcgg gccggtaggt gcgggcctcg gcgccgatga
      661 cgccttcgcc ggcgggcagc gtcgggcggg cagcgtcggc ctcggcgtga cgctgctcga
      721 tggcgacgag ctcgtcgagg cgggcctcga agtccgcgat ctgggtgtcc acctcggcga
      781 ggtggtcgcg ggcctcggcg aacgctgcgg cctcggtctc gttgaggtcg cgggactcgg
      841 cggtcggcgc ggcgaggacg tcggtgacgg cggtgtgggc ggcgtcgcgt tcggagcgca
      901 gctcggagat gcggtcgcgg aggtactgga tgaagtccat gggggtctcc ctacggggtt
      961 cggtgtgggt ttggggcccg gcaggtggga cgcaggtgtc ctcccaggtg gtgcccagtg
     1021 tgttccgggt ccggcgtggg ggggctgcgt gcgttccggc gtgcaggggg ggtcagacgc
     1081 cgagggcggc ggcctgagcg gcggcgagcg acaggggcat cccggtgcgc tcggcgatct
     1141 gttcgggttc cgggtcgccg ttgccggtgt cggggtcgat gccggcggcg gctcggaggg
     1201 cgatgacggt gacgtcgttg gcggggtagg tgacggcgga cacgtcgaac agtcggacct
     1261 cgaggatgcg gcgttcggtg tagtcgtcgt tccattcctg gcggagagcg atgaacgcgt
     1321 gggacatctg gtcgacgtcg ccgcgttcga gggtggagcg cagcttgacg gcgtcggggt
     1381 tgacgaggtc gagctccggg acgtcgacgc ggagtccgac gtcgtcctgg gcgagggtga
     1441 gggtgccggg cttggtgcgt gcgaggggga gcccgtcgtg gttgatgagg aaccggacgt
     1501 cgtcggcttc gcggagtgct ttggtgaatg ctccgggggc gatggtctcg gtccatcccc
     1561 aggggggtcc gccggcgacg tcgtagccga cgtcgaacac cgcggcgtag ccgccgaggc
     1621 cgacggtgtc gccgtcggag cggagctcga cgggctgctc gggtgaccgt cgggcttcga
     1681 cgagcgcccc gcgggaccgg cacgcgatgc ccgcggcgat caggtcgcct tcggtgaggt
     1741 cgagtcgggc tcggacctcg gcgggaaggg tgagtcggtc acggatcatg gagcctccgt
     1801 cgtggtgggc ttcgtggtgc gacgggtgaa ggtggtgtcg ccgtcgaggg gcggcaggtt
     1861 ctcgaggcgg cgcatctcgt cgttggtcag cagcggcacc ccggtggcgt cgaagatgcg
     1921 ggcggcgacc tcgtaggagg tgtagcgggc ggcgaggtcg gcgcggagga tggcgtcgag
     1981 gttgtggcgg acgcgttcac cgcggggcag cagctgcccg ataccggtct cgatgcgcac
     2041 caggtagggg gcgaagtcgc gggcggtcca gtcggaccac agctgttccc ggttgccgta
     2101 ggtgacgttc tggccggaca ccgcggagcc gatccattcg gcagggacac cgatcgcggc
     2161 ggcgatctga gctgaggacc attgcatcga gtcgaggaac tgcgactcgt tcggggcgag
     2221 ctggatgcgc tcgtagcgga gcccggagcc gagcacggcg ggttcgcggg ttccgcgggt
     2281 tgcttcttgg aatgctgctt tgatcttccg ggcgccggtg gcgtcgaggt tcgggtcctc
     2341 cgagtagagg atcgcggacg ggtgtccgcc gtcgtcgaac caccggttgc cgaactcttg
     2401 ggcggacagc ccggagtgga tggtcgcggc ggcctgaccg attggcgaca ggccttcggg
     2461 gcggccggcg acggtgaaca gcggcagatg ccacagctgt ccgtgcggcc acagcttctc
     2521 ggctgtgccg ttgactttgg cggtccaccg cttcgagcgg tccttctccc atttgacggc
     2581 cccggcggga agcagctcga cctggcgggg tgtacgcccg gcgatgacgc cagggggtcc
     2641 gctgcaccag ccgtacgcgt tgccggtcga caggagcgac acccacagcc ggtgcagcca
     2701 gtccggcagc ggttggccgg ggaacggctc gtcgagcagc gggtcgcggg ccacttcgac
     2761 gtcgccgcgg tactggtcga cgggcaacgt ggacccgatg ccggcgagga tgcggtagca
     2821 ggaccacacc gccgcgagct gcaacgcccg ttcgggggtg acggtctccc cgaacgatcc
     2881 cctgcgggcc gcccacatct cggccaccgg gtcggtgagg cttcggcgtt cgcggttcgg
     2941 acggatgagc cccatcagcg ttccagccac cagccgacga gcacgagcgc ggcgccggcg
     3001 gcgacgaacc cgacgacggg gctgaagatg aacccggcga cgacgaccag ggcgaggccc
     3061 acgagctcga gcaacgtcga cagcatcggc acctcctcag aaggcgtagg tcggggccac
     3121 ggtggcggcg gcacgttcgg tcggcagcaa cgcgcgggct ccggtgactg cttcgagggg
     3181 cgacaggtcg acgtcgctgg cccggccgtc ccacgcgaac ccgtcaccga ggcgacgttc
     3241 ccgcactccc gccaccgcgg tgtcgagcgg gccttgcccg tcgaggtgca cgagctgctg
     3301 ttcgcggacc gcggcgagga acccgccgca cgccgctttc cactcggcga gcccgaacag
     3361 gtggagccgg tcctcgacgc cggcagcctt gagggcgtcg agcacgacag ggacttgcgc
     3421 ggcggtcggt cccgcgttgt tgcagccgac ggcgacgggg tcccactcgg cgacgagctc
     3481 gacgagccgc cccggcagcc accggatagc gaggccggtg tcgaggacca tgaccgacgc
     3541 atccgcgatc gaaccccatc ccatgccgat cgacgacgac ttgccgtcga agctgaccga
     3601 ccagcacagc acgggttgcc ccgacgcgac gggggtcatg gcggtgaggg ggcggccgcc
     3661 ggcggtccac tcggtgaacg ggatgagggc gtcggtatcg acgaccggtg gcggcgccca
     3721 cacgcacagg tgctctcggg cgaagccggc gggaccgagg cgacgcagct gctcctcgag
     3781 gaactccatg ccctgcccgc gcccggcgag caccgacggg ttcgtggcac gccacaggga
     3841 ccggtcatcg acgtcgacgg gttcctgcac gacgttgccg gtgtcgtcga ggtggacgac
     3901 ctcggcggtg tgctcgaccc aaccgaaccg gccggggtca tcgccgagcg cccgccggcg
     3961 ctgcgaccac caccaggcgg accggcctgg gagccccgag gtccccatcg cgttcagctg
     4021 cccgttcggg ttcgcgagca acgtcgggga gatggcgtcg atgtgctcgt cgagagcgtg
     4081 ctgtgcttcg tcgaccacga cccggtcgac gtcgtcgacg ccacggccgc cgccaccgga
     4141 acgggtccgg taccagatcg tcgcgtcgtt gctgagctcg atcatctgct ggccggtgcc
     4201 ccgccagatg cgggtgatcc tcgaccgcag gtccggtgac gactccagca ccgacagcag
     4261 ccgctgctgc gcctgggtgg tcaacagcac ggcgtcgtgc acggtgtgca ggatgcgttc
     4321 accgcgttgc acaagccccc acagctcgac gacttcgatc tcgtcgccct tgccgttctg
     4381 gcgggccatc gcccgaccgg tcgtcgctgc cgcccaccgg ccgtcggagc gggtcgccat
     4441 gaggacttcg acgacgagcc gctgcgacgg gtccaacgtc ttggccgagt agtgctccca
     4501 catctcgatc gcggcgtggg cttcatccag gctttcgacg tcgggcggaa ccaccagcag
     4561 ccccggtgcg ggatcgacgt cgggaggcca gctggtccgc aacgctcacc tccgtcgatc
     4621 cctcgaggcg gtccaggagc tcgccgacga tccggcgttc cctcacgatc gccgcggctt
     4681 tcgacccgtc gggctccgct gcgagcagca cgccgagccg gtcgtagtcg gctcgcaggt
     4741 cggcggctcg gtccatgtgc gaccccctgg ctggtcaccc tccgtgacag tcgaccgccg
     4801 gagtcgctgc gggacacaca aaattcgacg aaggcggcga cggcgttcat gctgtgcccc
     4861 gatcgtcggc gaacacgtcg acgccgaggg cgacctcgag ccggcggcgc agctcgggcc
     4921 acggctcgac ccggccggtg ttgatctggt agagggtcgc cttgctgtag cc
//
LOCUS       DAOQOD010000210         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7192_length_4926_cov_11.108807, whole genome shotgun sequence.
ACCESSION   DAOQOD010000210 DAOQOD010000000
VERSION     DAOQOD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_4926_cov_11.108807"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLZ72_14460"
     CDS             <1..1222
                     /locus_tag="PLZ72_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37547.1"
                     /translation="YLPLVAGVAAVVAVRTWRRPEVRATRARVPWLTGLAALLWVPPL
                     VDLVAGSHNPWLLTRAMAGGGEPGAGVPVVAAAAALGPAHGTVDSMWLPRSATAVEWL
                     IVGVSVVVVVLSGWVAPRDHWSRSWIIAFGSAFGVWTLLASRAPATDGLIPTAYTRPL
                     WPLASCLTFGVAAAWWARRPSRLAGAWWVPVLVTAVWVLSVPRGYVGLDPATRVRAGQ
                     VVADLAAADRLPDRLNVRASGFVAGWFVAPAVTAELDRRGIANGVGTDDEWDVASMTD
                     RPRPLRQACDLQLGDEPADSTGGGPLLAAPSPLTRSELRRLAALRSILARRYPDPTPS
                     RVAADAAAHGGPQLDLSDGTTALLADGRFADLVMAGALDGVSTRDGDVAEYVELAHRA
                     DPAWAELTAALDDC"
     gene            1309..3009
                     /locus_tag="PLZ72_14465"
     CDS             1309..3009
                     /locus_tag="PLZ72_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37548.1"
                     /translation="MPGVDSEAEAPDGIATPEVTTDLRWGRTDALGLAVVTLVLALPV
                     AGLMRYQGPPMEEGFMLAFPEQILNGSWPHRDFLHLYGPGSLWVLAAVYKVLGVSLTV
                     ERSVGLVQHAAVAYGLFVLLRPFGRVVATSSAVVSVLVLIGPLGLSAMAWNGALAFGI
                     WGLVAGTTAARRYRGGSTDRSWLVPLAGVLSGIALLYRPDLVLAVGLGLAALWFDLPP
                     QRRRPLLWGTLGALALYVPHVLISGVGDSFRGMFLEPVFQLRGGRSLPVPPSWGEVDG
                     FLQRAGVLRTSGWPFPMPALSQQIFLWFALIPLSIALVVFAAWRLRRREPGSERAIAL
                     WAPALFSAALLQQAVQRPDTAHLSWVTGITFALAIPAIASLVASVRPAWPSAAGVALG
                     IGTVAVVLVAVIPFYPLRTYVDLVGQSAGRNRFGFPITNDGRTFYYGSAEAAADAQAV
                     VDSLRERAEPGDSLIVGPVDLSRTNYSDSFFYYLFPDLVPGTRYIEMDPGIADADDSG
                     LADELERNDWLIVSDAWSGWTEPNDSAGHGSDAANRVVAEHYCPVTDAGTFRLLERCR
                     "
     gene            complement(3040..>4926)
                     /locus_tag="PLZ72_14470"
     CDS             complement(3040..>4926)
                     /locus_tag="PLZ72_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37549.1"
                     /translation="SIPVSDPPPVVRRRRRGDADELIPTRTDRRELLLQIAIVVVAAA
                     PALAATLSVIGIHWVPGGDQAIEVLRISDVGGPHTPLVGPWSRWGWAHPGPALFWLVA
                     PTQHLFGNDGVLLACGLLSTASAAGIAAVAIRRGGTVLGAITALGVVGFLGGMGLSTA
                     VDPWNPYVALLPFLLFVLLVWSVVCDDLVMAPAAAVVGSFCVQAHVGFLPLVGGLSAL
                     AFVAVAVRHLRAHRAAPEGGTRSDTGSGGLLAAVRRHRLLSTFLATVVAGGLMWLAPL
                     WDQLFGSGNLSALLSYARSPESDTAGWSKAFGIMSGQLRLPAPWMGANEASAAGLTLT
                     AGWAWGVGAVLLLVVAAVVAVRRRHVDVAILDGIAVVAIGLAVLSTARMTGFVAPYMT
                     QWWWIVSFVALLALVWTVVSLLDLADARALAVGALGVAVVVGGFVVVRDVPVDAPVPT
                     LSRALSELVGPTAAALDRDHRYVVHTVDERNWGGAGPGLQLALEQLGYHVFVGPTDLT
                     DIQYGARRVLEPAVGDGVITIVGLDSMGNGWVPPDGARELARYDPLSAAERREFDESQ
                     ASMHERMSAALGSEAPEGVLSMDSLHHAMLARASGVSQAEIDRMKELQAPGSGFAVFL
                     SPPA"
BASE COUNT          630 a         1817 c         1793 g          686 t
ORIGIN      
        1 ctacctgccg ctcgtcgccg gggtggcggc ggtcgtcgcg gtccggacgt ggcggcgccc
       61 cgaggtccgc gcgacccggg cccgggtgcc gtggcttacc ggtcttgccg ccctgctgtg
      121 ggtccccccg ctggtcgacc tcgtcgccgg aagccacaac ccgtggctgc tcacccgtgc
      181 gatggccggc ggcggggagc ccggtgcggg ggtgccggtc gtcgctgcgg ccgccgcgct
      241 cggtccggcc cacgggaccg tggactcgat gtggttgccc cgctcggcca ccgcggtcga
      301 gtggttgatc gtcggcgttt cggtggtcgt cgtggtgctg agcggttggg tcgccccccg
      361 cgaccactgg agccgcagct ggatcatcgc gttcggctcc gccttcgggg tgtggacgct
      421 gctcgccagc cgggccccgg ccaccgacgg cctgatcccc accgcctaca cccgtccgct
      481 gtggccgctg gcgtcgtgtc tgaccttcgg cgtggccgcc gcgtggtggg cgcgccggcc
      541 gtcacgcctg gccggagcgt ggtgggtccc ggtgctcgtc accgcggtct gggtgctgtc
      601 ggttccccgg ggctacgtcg gcctggatcc cgccacacgc gtccgggccg gccaggtcgt
      661 cgcggacctc gccgccgctg atcgcctgcc cgaccgactg aacgtcaggg cctcggggtt
      721 cgtcgccggg tggttcgtcg cccccgcagt caccgccgag ctcgaccgtc gcggcatcgc
      781 caacggcgtc ggcaccgacg acgagtggga cgtcgcatcg atgaccgacc ggccccggcc
      841 gctgcggcag gcctgcgacc tgcagctggg cgacgagccc gccgacagca ccggaggcgg
      901 cccgctcctg gccgctccga gccccctgac ccgttccgag ctgcgccgtc tggccgcgct
      961 ccggtcgatc ctggctcgcc gctacccgga cccgacgccg tcacgggtcg ccgcggacgc
     1021 cgccgcgcac ggcgggccgc agctcgacct cagcgacggg acgaccgcgc tgctcgccga
     1081 cggacggttc gccgatctgg tgatggcggg cgccctcgac ggcgtgtcga cccgcgacgg
     1141 cgacgtcgcg gagtacgtgg agctggccca ccgggccgat ccggcctggg ccgagctcac
     1201 cgcggcgctc gacgactgct gagcgtcgcc actcgcagcc tccggtcccg gtgggccggg
     1261 ctccggtcga tgtcggaccg acggcaccgg ccgcgcgaga ctcagccggt gccgggcgtg
     1321 gacagcgagg ccgaggcgcc cgacgggatc gccaccccgg aggtgacgac cgacctgcgt
     1381 tgggggcgga ccgacgcgct cggtctggcc gtggtcaccc tggtcctggc gctgccggtg
     1441 gcgggactga tgcgctacca gggtcctccg atggaggagg gcttcatgct ggccttcccg
     1501 gagcagatcc tgaacgggtc gtggccgcac cgcgacttcc tgcacctcta cggcccggga
     1561 tcgctgtggg tgctcgccgc ggtgtacaag gtgctcgggg tcagcctgac cgtcgagcgc
     1621 tcggtcgggt tggtgcagca cgcggcggtt gcctacggcc tgttcgtcct gctgcgcccg
     1681 ttcggacggg tcgtcgccac ctcctcggcg gtcgtgtcgg tgctggtgct catcggtccg
     1741 ctcgggctgt cggcgatggc gtggaacgga gcgctggcgt tcggcatctg gggcctggtc
     1801 gcgggtacga cggcggcccg ccgctaccgc ggcgggtcca ccgaccgcag ctggctggtg
     1861 ccgctggccg gcgtgctgag cggcatcgcg ctgctgtacc gccccgacct ggtgctcgcc
     1921 gtcggactgg gcctggccgc gctctggttc gacctgccac cgcagcgacg tcgaccgctg
     1981 ctgtggggga ccctcggcgc cctggcgctg tacgtccccc acgtcctgat ctccggggtg
     2041 ggcgactcgt tccgggggat gttcctcgag ccggtgttcc agctccgagg gggccgatcc
     2101 cttcccgttc caccgtcgtg gggtgaagtc gacggcttcc tgcaacgagc cggcgtcctt
     2161 cgcaccagcg ggtggccgtt cccgatgccc gcgctgtccc agcagatctt cctgtggttc
     2221 gcgctcatcc cgctgagcat cgccctcgtc gtgttcgcgg cctggcggct gcggcgccgc
     2281 gaaccgggct cggagcgggc gatcgccctg tgggcccccg cgttgttctc cgccgctctg
     2341 ctccaacagg cggtgcaacg ccctgacacc gcccacctgt cgtgggtgac cggcatcacc
     2401 ttcgccctgg cgatccccgc gatcgcgtcg ctcgtcgcct cggtgcggcc cgcctggccg
     2461 tcggccgcgg gcgtcgccct gggcatcggc acggtcgcgg tcgtgctggt ggcggtgatc
     2521 ccgttctacc cgctgcgcac ctacgtcgac ctggtcggcc agagcgccgg gcgcaaccgc
     2581 ttcggcttcc cgatcaccaa cgacgggcgc accttctact acggcagcgc ggaggcggcc
     2641 gcggacgccc aggcggtggt cgacagcctc cgggagcggg ccgagccggg tgactcgctg
     2701 atcgtcgggc ccgtcgacct gtcgcgcacc aactacagcg actcgttctt ctactacctg
     2761 ttccccgacc tggttcccgg cacccgctac atcgagatgg accccggcat cgccgacgcg
     2821 gacgactcgg ggttggccga cgagctggaa cgcaacgact ggctgatcgt ctccgacgca
     2881 tggagcgggt ggaccgaacc gaacgacagc gcgggtcatg gcagcgacgc ggccaaccgg
     2941 gtcgtggcgg agcactactg cccggtgacc gacgccggga cgttccggct gctcgagcgc
     3001 tgtcgttgag cggcgctgtc gctgaatgag gctgtccggt caggccgggg gagacaggaa
     3061 caccgcgaaa cccgacccgg gagcctggag ctccttcatg cggtcgatct cggcctggga
     3121 gacacccgag gcccgcgcca gcatcgcatg gtggagcgag tccatcgaga gcacgccctc
     3181 gggggcctcg gagccgaggg cggcactcat ccgttcgtgc atcgacgcct gggactcgtc
     3241 gaactcgcgg cgctcggccg cgctcagcgg gtcgtagcgg gcgagctcgc gggcgccatc
     3301 gggcggcacc caaccgttgc ccatcgaatc cagcccgacg atggtgatca ccccgtcgcc
     3361 gaccgctggc tcgaggaccc gccgcgctcc gtactggatg tcggtcagat cggtgggtcc
     3421 gacgaacacg tggtagccca gctgctccag cgcgagctgc aggccggggc ccgctccgcc
     3481 ccagttgcgc tcgtcgacgg tgtgcacgac gtagcggtgg tcgcggtcga gggccgccgc
     3541 ggtcggtccg accagctcgg acagcgctcg tgacagggtc gggaccgggg catcgaccgg
     3601 tacgtcccgg acgacgacga acccgccgac gacgaccgcc acccccagcg ccccgacggc
     3661 gagggcgcgg gcgtcggcga ggtcgagcag cgagaccacg gtccacacca gcgccaggag
     3721 ggcgacgaag gagacgatcc accaccactg ggtcatgtac ggggcgacga acccggtcat
     3781 ccgggcggtc gacagcacgg cgaggccgat cgcgaccacg gcgatcccgt cgaggatggc
     3841 gacgtcgaca tggcgtcgac ggaccgcgac cacggcggcg acgaccagca ggagcaccgc
     3901 gccgacaccc cacgcccaac cggcggtcag cgtcagtccg gcggcgctgg cctcgttcgc
     3961 gcccatccac ggggcgggca ggcgcagctg gccgctcatg atgccgaacg ccttggacca
     4021 ccctgccgtg tcggactcgg gggatcgtgc gtagctcagc aacgccgaga ggttcccgct
     4081 gccgaacagc tggtcccaca gcggcgcgag ccacatgagc ccgccggcga cgacggtggc
     4141 caggaacgtg ctcagcagtc ggtgccgacg gaccgcggcg agcagcccgc cggatccggt
     4201 gtccgagcgg gtcccgccct cgggcgcagc ccggtgggca cggaggtggc ggaccgcgac
     4261 ggccacgaac gccagagcgg acagaccccc caccagtggc aggaagccga cgtgggcctg
     4321 gacgcagaac gacccgacca ccgcggccgc cggcgccatc accaggtcgt cgcagaccac
     4381 gctccacacc aggagcacga acagcaggaa cggcagcagc gccacgtacg ggttccacgg
     4441 atcgacagcg gtgctcagac ccattccacc caggaacccg accacgccga gcgcggtgat
     4501 cgctccgagg acggtgccgc cacgccggat ggccaccgca gcgatgcccg ccgcgctggc
     4561 cgtgctcagc agaccgcagg ccagcagcac cccgtcgttg ccgaacaggt gctgggtggg
     4621 ggcgaccagc cagaacagcg cgggtccggg atgcgcccaa ccccaccgcg accaggggcc
     4681 caccagcggg gtgtgcgggc ctccgacgtc ggagatgcgc aggacctcga tggcctggtc
     4741 tccaccgggg acccagtgga tgccgatcac cgagagggtg gccgccaggg ccggcgccgc
     4801 ggcgacgacg acgatggcga tctgcagcag cagctcgcgc cgatcggttc gggtcgggat
     4861 cagctcgtcc gcgtcacccc gacggcgtcg tcggaccacg ggcgggggat cggagaccgg
     4921 gatgct
//
LOCUS       DAOQOD010000211         4915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7215_length_4915_cov_10.126543, whole genome shotgun sequence.
ACCESSION   DAOQOD010000211 DAOQOD010000000
VERSION     DAOQOD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7215_length_4915_cov_10.126543"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1675)
                     /locus_tag="PLZ72_14475"
     CDS             complement(17..1675)
                     /locus_tag="PLZ72_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37550.1"
                     /translation="MSEVPISSAGPTDEASLRPLDEPAMRQAVVDMRRRRQRRRLGNH
                     EWGELAYRVYTTTLAAIVAVVFASGLIGDDRLDASARADFLRQAPAWFGLGAAVVVLI
                     GIRSGARGGPLALERQDVHHLLLAPVDRSFVLRRPAVGVLSYACLGGAILTGLGAGLF
                     DQRLGGASLPWFACGALFGATVAALAVGAGLLTSSRLTSRWVPLGIGWVLVLWALGDA
                     TGYAPTAPTTWLGSIVVWPLSVDLLGLVPVGVAIALPLIGLLTIGGLSVEAATRRTAL
                     VGQLRFAVTQQDLRTVVLLRRQLAQERPRTRPWFPATPGPVARRFPVFARDMRSVARW
                     PMVRVARVLVLGGSIGVVARATWAGTTPLVLVMGALAYVVALDAIEPLAQDVDHPGLL
                     GSLPEIEGMVMVKHLAEPVLVMVGVGLVAVGAAYAVSPDPLVWEVGLPMVLPAAFAGV
                     GGAAVTVVSEVDVSTGDDAVMPPEVAGPRLLFRTVWPPAVAVIGMLPLLAARVASEAG
                     KDPAAAVTTWAIPVVVLGMLIVGWVRFRADIHESIATATGGGAA"
     gene            complement(1729..2931)
                     /locus_tag="PLZ72_14480"
     CDS             complement(1729..2931)
                     /locus_tag="PLZ72_14480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA37551.1"
                     /translation="MSGPRDVADLAAAVGLEPARHRRADVESLVGIPHTDMLRWWRAI
                     GFAEVPEEEVAFGDDDIRMAVALGALLGGGAVDDRDVLRLARVLGSSFSRIAEAQVAL
                     LTDAARAGRADDGSPPPDVLDIATALHDAELLDLLEQSIVYVWRRHLLAALGRRLRVE
                     EDAAAEIRSGAAPPPTADEGEGDGEVDAPDPLDSDHGPSATTVGFVDIAGFAKMTRRI
                     DVDDLAEVVDAFEAAALEVIAEHHGRVVKFIGDAVMYVARDLPSGVRIGLELQGRAAA
                     AELPVELHCGVAHGPTITMGGDVFGPAVNLASRLTGVARRGTVVIPRECARELADSPD
                     LQVRSVRRLHDLKGIGRTRLSTVRWADRPGTEQPSPSADTDGDGNHGDAIEGVAAADD
                     AGGPTDRG"
     gene            complement(3000..4574)
                     /locus_tag="PLZ72_14485"
     CDS             complement(3000..4574)
                     /locus_tag="PLZ72_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNA37552.1"
                     /translation="MAELVDQEGSVRARSVTGSRSPRLRRRAVRRVVAGLAVLGSLAV
                     VTAACATPPDLVALADPGGPVTVAAPDVIGPMRVRGGDLIDGAGRTVLMHGVNSVAKW
                     EPWVTPVDAPDGFDRDTLGPDDLAALDRDGYNAVRLGVWPAALMPAPGQVDQEYLDRV
                     AVTVDALSAHGIWVLLDLHQDVFHGMPEWATTPAAAALSPEADPGLAAAIGWSAAYFS
                     PRSLKQWDDWWANAEVAPGLGVVDAYAQGAAALADRFDSDPAVIGLELINEPFPGSPA
                     LQCLIGDCPDLDATVGARNAQISAVIRASAPEMPLWWEQETLFPNFTDATPPQPDVPP
                     TADGRQVVSSFHIYCLGTDSGKPELPDPTSQAYCDAQISQGVNRGLIVGRSWGAPAVM
                     TEFGASNSPLNSTLTTRMADEQLFSWFHWHHGTYPSVVESQLVRTYAQATAGTPLEQR
                     FEPSTGSFTFRYRPDPSVEAPTSIVVPQRVYPDGYRVEVSGGTVTSAPNSGRLTVLAD
                     GIGEVTVRVTRSADGV"
     gene            4659..>4915
                     /locus_tag="PLZ72_14490"
     CDS             4659..>4915
                     /locus_tag="PLZ72_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37553.1"
                     /translation="MAAPVHDDAPSDSGPDHGSRPPTTTGDGGHRRRPGLQMILIALG
                     AVAVLGIGLVAATRGTSEKPWAGQILPEAQPKPEFVLTGTDG"
BASE COUNT          762 a         1776 c         1799 g          578 t
ORIGIN      
        1 ccacctccgg tggcggtcac gcggcgccac ctccggtggc ggtcgcgatc gactcgtgga
       61 tgtcggcccg gaaccgcacc caaccgacga tcagcatccc caggaccacg acggggatcg
      121 cccacgtcgt caccgcggcc gcgggatcct tgccggcctc ggacgcgacc cgggcggcga
      181 ggagggggag catgccgatc accgcgacgg ccgggggcca cacggtccgg aacagcagtc
      241 ggggcccggc gacctcggga ggcatcaccg cgtcgtcacc cgtgctgacg tcgacctcgg
      301 acaccaccgt gaccgcggct ccccccacac cggcgaaggc ggccggcagc accatgggca
      361 ggccgacctc ccacaccagc gggtccggcg acaccgcata ggcggcgccc accgccacga
      421 gacccacgcc gaccatcacc aggaccggct cggcaaggtg cttgaccatc accatgccct
      481 cgatctcggg cagcgaaccc agcagaccgg ggtggtcgac gtcctgggcg agaggttcga
      541 tggcgtcgag cgcgaccacg taggcgagag cgcccatcac cagcaccagg ggagtcgtcc
      601 ccgcccacgt cgcccgggcg accacgccga tcgatccccc gagcaccagc acgcgggcga
      661 cccggaccat cggccagcgg gcgacgctgc gcatgtcacg ggcgaagacc gggaaccgac
      721 gagcgaccgg ccccggagtc gcggggaacc agggtcgggt ccgtgggcgc tcctgggcga
      781 gctgacgtcg cagcaggacg accgtgcgga ggtcctgctg ggtgacggcg aagcggagct
      841 ggcccaccag cgcggtccga cgggtcgccg cctcgaccga caacccgccg atggtgagca
      901 ggccgatcaa cggcagggcg atcgccacgc cgacgggcac cagcccgagc aggtcgacgc
      961 tgagcggcca cacgacgatc gagccgagcc aggtcgtcgg agcggtgggc gcgtacccgg
     1021 tggcgtcccc cagagcccac agcacgagga cccaaccgat tcccaggggc acccagcgcg
     1081 atgtcagccg cgacgaggtg aggagcccgg cacctaccgc cagcgcggcg accgtggcgc
     1141 cgaagagcgc gccgcacgcg aaccacggga gcgacgcgcc gcccagccgt tggtcgaaca
     1201 gacctgctcc caacccggtc aggatcgcgc ctccgagaca ggcgtagctc agcaccccga
     1261 ccgcggggcg ccgcaggacg aaggaccgat ccaccggagc caggagcagg tggtggacat
     1321 cctgtcgctc cagggccaga ggaccccctc gggcgccgga gcggatgccg atgagcacga
     1381 cgaccgctgc gccgagcccg aaccaggcgg gcgcctggcg aaggaagtcg gcccgggccg
     1441 acgcgtcgag acggtcgtcg ccgatcagac ccgacgcgaa caccaccgcg acgatggcgg
     1501 ccaacgtggt cgtgtagacc cggtaggcga gctcacccca ctcgtggttg cccagacgtc
     1561 gtcgctgacg acgacgtcgc atgtcgacga cggcctgacg catcgccggt tcgtcgaggg
     1621 gccgcaacga tgcctcgtcg gtcggtccgg ccgacgatat ggggacttcc gacatcaccg
     1681 gcagtctgga cctcgggccc ggcgtccgtc gagacgggga ccgggcgctc acccgcggtc
     1741 agtcggccca ccggcgtcgt cggccgcggc gaccccctcg atggcgtcgc cgtggttgcc
     1801 gtcgccgtcg gtgtccgccg agggagaggg ctgctcggtc ccgggacggt cagcccagcg
     1861 caccgtcgac agcctggtcc gaccgatccc cttcaggtcg tggaggcgcc tcacggagcg
     1921 gacctgcagg tcgggtgagt cggccagctc cctggcgcac tcacggggga tcacgacagt
     1981 gccccgtcga gccaccccgg tcaaccggct ggcgaggttc accgccggtc cgaagacgtc
     2041 gccacccatg gtgatcgtcg ggccgtgggc cacaccgcag tgcagctcga cggggagctc
     2101 ggccgcggcg gcacggccct ggagctcgag gccgatgcgg acccctgacg gcaggtcgcg
     2161 ggccacgtac atgaccgcgt cgccgatgaa cttgacgacc cggccgtggt gctcggcgat
     2221 gacctccagc gccgcggcct cgaacgcgtc gaccacttcg gccaggtcgt cgacgtcgat
     2281 ccgacgggtc atcttggcga accccgcgat gtcgacgaag ccgaccgtcg tcgcggacgg
     2341 accgtgatcg gagtcgagcg gatcgggtgc atcgacctcg ccatcgcctt cgccctcgtc
     2401 ggctgtcggg ggcggagcgg cgccagaacg gatctccgcc gcggcgtcct cctcgacgcg
     2461 gagtcgccgg ccgagagcgg cgagcaggtg acggcgccac acgtagacga tcgactgctc
     2521 gagcaggtcg agcagctcgg catcgtgcag cgcggtggcg atgtcgagca catccggagg
     2581 aggtgacccg tcatcggcgc gacctgcgcg ggccgcgtcg gtgaggagcg ccacctgcgc
     2641 ctcggcgatc cgggagaacg acgaaccgag gacccgcgcg aggcgcagga cgtcgcggtc
     2701 gtcgacggcg ccgccgccga ggagggcgcc gagcgcgacg gccatgcgga tgtcgtcgtc
     2761 gccgaaggcg acctcctcct cggggacctc ggcgaatccg atcgcccgcc accagcgcag
     2821 catgtcggtg tggggtattc cgaccagcga ctcgacgtcg gcccggcggt gcctcgccgg
     2881 ctccagcccg accgcggcgg cgaggtcggc gacgtcgcgg ggcccactca tcccggcgat
     2941 cgtaacggcg accggacggg ctgacccgtt cgggtccggc ggacgtccga ccgggtcggt
     3001 cagaccccgt cggccgagcg ggtcacgcgc accgtgacct cgccgatgcc gtcggccagc
     3061 accgtcagtc ggcccgagtt cggcgccgat gtcacggtcc cgccggacac ctcgacccgg
     3121 tagccgtcgg ggtagacacg ttggggcacg acgatcgacg tcggtgcctc gaccgacgga
     3181 tcgggtcggt agcggaaggt gaacgaaccg gtggacggct cgaaacgctg ctcgagcgga
     3241 gtccccgcgg tcgcctgggc gtaggtgcgg accagttggc tctcgaccac cgagggatag
     3301 gtcccgtggt gccagtggaa ccacgagaag agctgctcgt cggccatccg ggtggtgagc
     3361 gtggagttca gcgggctgtt ggatgcgccg aactcggtca tcaccgccgg ggcgccccag
     3421 gaccggccga cgatcaagcc ccggttcacc ccctgggaga tctgggcgtc gcagtaggcc
     3481 tgcgaggtcg gatcgggcag ttccggcttc cccgagtcgg tgccgaggca gtagatgtgg
     3541 aacgacgaga cgacctgtcg gccgtccgcg gtcgggggca cgtccggctg aggcggtgtg
     3601 gcgtcggtga agttggggaa cagggtctcc tgctcccacc acaggggcat ctcgggtgcc
     3661 gaggcgcgga tgaccgccga gatctgcgcg ttgcgggcgc cgacggtggc gtcgaggtcg
     3721 gggcagtcac cgatcaggca ctgcagcgcg ggactgcccg ggaagggttc gttgatcagc
     3781 tcgaggccga tgaccgcggg gtcggagtcg aagcggtccg cgagagcggc cgccccttgg
     3841 gcgtatgcgt cgaccacgcc gaggccgggg gcgacctcgg cgttggccca ccagtcgtcc
     3901 cactgcttca gggagcgggg tgagaagtac gcggccgacc acccgatcgc tgcggccagg
     3961 ccgggatcgg cctccgggct cagggccgcg gcggcgggcg tcgtggccca ctccggcatt
     4021 ccgtggaaga cgtcctggtg caggtcgagc aggacccaga tgccgtgcgc ggacaaggcg
     4081 tcgacggtga cggcgacccg gtcgaggtac tcctggtcga cctggccggg agccggcatg
     4141 agggccgcag gccacacgcc caggcggacg gcgttgtagc cgtcgcggtc cagcgcggcc
     4201 aggtcgtcgg ggccgagggt gtcgcggtcg aacccgtccg gggcgtcgac aggggtgacc
     4261 cacggctccc acttggccac cgagttgacc ccgtgcatca gcacagtgcg accggcgccg
     4321 tcgatgaggt ccccgccccg cacccgcatg ggtccgatca catcgggagc ggccacggtc
     4381 accggccctc ccgggtcggc caacgccacc aggtccggag gcgtggcgca cgccgcggtg
     4441 acgaccgcca gcgaaccgag cacggcgagc ccggcgacca cgcgtcggac cgcccgccgc
     4501 cggagccgcg gcgaccggct cccggtcacc gaccgcgccc gaaccgaacc ctcctgatcc
     4561 accagctccg ccatggcggt gaactgtacc ggtacgcccg ccgggcgccc aggggcgact
     4621 gccgcagcgg ggggacgcac tggtaccgtc acggagccat ggcagcaccg gtccacgacg
     4681 acgccccgtc cgattccggc ccggatcacg gctccaggcc gcccaccacc accggcgacg
     4741 gtggccaccg tcgccggccc ggactccaga tgatcctgat cgccctcggt gccgtggccg
     4801 tgctgggcat cggtctcgtg gcggcgacca ggggaacgtc cgagaagccc tgggcgggtc
     4861 agatcctgcc cgaggcccag cccaagcccg agttcgtgct gaccggcacc gacgg
//
LOCUS       DAOQOD010000212         4891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7288_length_4891_cov_11.299007, whole genome shotgun sequence.
ACCESSION   DAOQOD010000212 DAOQOD010000000
VERSION     DAOQOD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7288_length_4891_cov_11.299007"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLZ72_14495"
     CDS             complement(<1..407)
                     /locus_tag="PLZ72_14495"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HNA37554.1"
                     /translation="MAAVIGDPVTHSLSPRLHNAAFRAVGLDWVYVALPVARGQARAA
                     VDGARALGLRGLSVTMPHKEAVIDSLDGLTSAAADLGAVNCILRAPHDDGLLLGDNTD
                     GQGFIRGLRADLGLDPAGLRCAVVGAGGAGRAVA"
     gene            complement(437..1498)
                     /gene="mltG"
                     /locus_tag="PLZ72_14500"
     CDS             complement(437..1498)
                     /gene="mltG"
                     /locus_tag="PLZ72_14500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNA37555.1"
                     /translation="MVLPRATRGGPRLVAVFGAIALAVGIVVFSAVFWVTRQISPSGG
                     QGKRVAEVIVPTGASTDAIATLLDDAGVITNARMFRWYTSWKSAGPWDAGRYVEFRRN
                     SSFDEAIEVLDDGPVPVDAVTVRIPEGRRLVDALADINAAFPNLSVAELQATLDSGSV
                     TSRYKPAEIPNWEGFLFPDTYEFFDDATAQEILQTMATKMDDVLDSLGYDKAESLQGR
                     TAYELVTIASLVERETGQPPEERGKIARVIMNRLDDGEPLGIDAANLYGLGRTSGELT
                     KADLAVESPYNVRKNKGLPPTPICLPGEASLRAAIEAPAGTWRYYVLTTKDPPTHLFT
                     DSYREFQKAKADAQARGVF"
     gene            complement(1672..2139)
                     /gene="ruvX"
                     /locus_tag="PLZ72_14505"
     CDS             complement(1672..2139)
                     /gene="ruvX"
                     /locus_tag="PLZ72_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNA37556.1"
                     /translation="MDRPHRTAGPVRALGLDLGSQRIGVALSDSDATLALPYDVVVRT
                     GDRDRDHRRIADIVAETGAEVVVVGLPLSLDGGEGPMARRYRAEARRIGATLGVPVET
                     YDERLTTVTAERALRESGLSGRARRRVVDQVAASVMLQSWLDRRAARGEEPPE"
     gene            complement(2141..4801)
                     /gene="alaS"
                     /locus_tag="PLZ72_14510"
     CDS             complement(2141..4801)
                     /gene="alaS"
                     /locus_tag="PLZ72_14510"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNA37557.1"
                     /translation="MTEPTAPRGDTSADVARPPSDSAGGWTADRLRRAWTEFFNERAH
                     TVVASGGLIPTHPSAPMFTNSGMMPFVPMFLGEEPVPYRPPRAVSVQKCVRAGGKHND
                     LDAIGRSQRHLSFFEMLGNFSFGDYFKSEAIAWAWEFVTDVLGIDGERIWVTVHVSDD
                     EAERIWIDQVGFPAERIQRLDKDNFWEMGETGPCGPSSELFFDYGPEAGPDGGPANPA
                     AEDRYVEIWNLVFQQYFRGADGELTDLPTRNIDTGAGLERILAVLAGSPSLYSADVLA
                     RLVTRAEEVTGRSVGDGELSAIALRLLADHTRTATFLVADGVVPSNEDRGYVLRRIIR
                     RAVRFAYMLDVDDLVMAPMVECCIDIMGGAYPDLVEGRGRIVEAISREEEGFRRTLAR
                     GSVLLDSALDEIPAGGELDGSVAFALHDTYGFPLEVTQEMADLRGVSVDLAGFDADMA
                     AQRERSRTSSKRGAVDAGELADVARRLLAERGPTEFTGRDEDRSDATVLAVVDDAVVL
                     DRTPFYAESGGQVGDTGTITTATGTLRVTDTTYVLPGVHRHTVEVIDGTVTEGQAAVA
                     AIDTDRRAAIRRNHTGTHILHWALREVLGDHVKQQGSWVGPDRLRFDFAHPGAVSGEE
                     IRRIEDLANAEILDNAPVRHFETTMDEARELGALMFFGDKYGDVVRVLEAGRHSTELC
                     GGTHVRALGDIGPLKVVSETSIGSNIRRIEAVTGTGPIERLRQEEDRLRAAADLLGVP
                     VDDLQGAIEKRVTELKAARDELKVLRRQLAGNQAEDLAGSAVDGVVVARVDAESRDEV
                     RDLAVALRDRPGIRAVVLGASPGGKGVALVAAVGSDSGLNAAELIAEAARTVGGGGGK
                     GADLAAAGGKSPERLDEALDQARAAAGLTG"
BASE COUNT          665 a         1724 c         1808 g          694 t
ORIGIN      
        1 gccaccgccc ggcccgcccc gcccgctccg acgaccgcgc agcgcaggcc ggcgggatcg
       61 aggccgaggt cggcgcgcaa ccccctgatg aacccctgtc cgtcggtgtt gtcgccgagc
      121 agcaggccgt cgtcgtgggg ggcgcgcagg atgcagttga ccgcgccgag atctgcggcc
      181 gccgaggtca gcccgtcgag cgagtcgatc acggcctcct tgtgcggcat cgtcaccgac
      241 agcccgcgca gcccgagagc acgggcgccg tcgacggcgg cacgggcctg cccgcgtgcc
      301 acggggaggg cgacgtacac ccagtccagg ccgacggccc ggaacgccgc gttgtgcagc
      361 cggggcgaca gcgagtgcgt gaccgggtcg ccgatgaccg ccgccacacg ggtggccccg
      421 gtcggggcgg acgcgttcag aacacccctc gggcctgggc gtcggccttg gccttctgga
      481 actcccggta ggagtcggtg aacaggtgcg tcggcgggtc cttggtggtc agcacgtagt
      541 agcgccaggt ccccgccgga gcctcgatcg ctgctcggag cgaggcttcg ccgggcaggc
      601 agatgggcgt gggtggaagc cccttgttct tgcggacgtt gtagggcgac tcgaccgcca
      661 gatcggcctt ggtgagctcg ccggaggtcc gccccaaccc gtagaggttg gcggcgtcga
      721 tcccgagcgg ttcgccgtca tcgaggcggt tcatgatcac ccgggcgatc ttgccccgct
      781 cctcgggcgg ctgaccggtc tcgcgctcga ccagggacgc gatcgtcacc agctcgtagg
      841 cggtgcgacc ctggaggctc tcggccttgt cgtagcccag gctgtcgagc acgtcgtcca
      901 tcttggtcgc catcgtctgg aggatctcct gggcggtggc gtcgtcgaag aactcgtagg
      961 tgtcggggaa caagaacccc tcccagttgg ggatctcggc cggcttgtag cgcgacgtga
     1021 ccgagccgga gtcgagggtg gcctgcagtt cggccacgct caggttgggg aacgcggcgt
     1081 tgatgtcggc cagggcgtcg accaggcgac gcccctcggg gatccgcacc gtgacggcgt
     1141 cgaccggcac cggaccgtcg tcgagaacct cgatcgcctc gtcgaagctc gagttccggc
     1201 ggaactccac gtagcgaccg gcgtcccatg gaccggcgga cttccagctg gtgtaccagc
     1261 ggaacatgcg ggcgttggtg atgaccccgg cgtcgtcgag aagggtcgcg atggcgtcgg
     1321 tgctggcccc ggtcggcacg atcacctcgg ccacccgctt gccctggcct cccgacgggc
     1381 tgatctgacg ggtcacccag aacaccgccg agaacacgac gatgcccacc gccagggcga
     1441 tcgcgccgaa cacggcgacc agacggggac cgccgcgggt ggcccggggc aggaccacgt
     1501 actcgtcgtc ggccgtggat cctgaccggc caccggtgga gcgggatcgg cccggtgtct
     1561 cgctcggcga gggacccggg ggccgacggt tgcgccggac cggacgcgga tcgtcggacg
     1621 ccggatcccc gctcgatggc cgctccggcg tgggtcgcgg gtcgtcgtca gtcactcagg
     1681 gggttcctca cctcgggctg ctcgtcggtc gagccagctc tgcaacatca ccgatgcggc
     1741 cacctggtcc acgacccggc gccgggcccg gcccgacagg ccgctctccc ggagggcccg
     1801 ttccgcggtg accgtcgtca ggcgctcatc gtaggtctcc acgggaacgc cgagggtggc
     1861 gccgatccga cgggcctcgg cccggtaccg gcgggccatc gggccctctc ccccgtcgag
     1921 cgacagcggg aggccgacga cgaccacctc ggcgccggtc tcagcgacga tgtcggcgat
     1981 gcgacggtgg tcccggtccc ggtcgccggt gcgcacgacg acgtcgtagg gcagggcgag
     2041 cgtggcgtcg gagtcagaca gggcgacccc gatgcgctgg ctgccgaggt cgagtccgag
     2101 agcccgcacc ggcccggcgg tgcggtgcgg tcgatccacg tcagccggtg aggccggccg
     2161 cggcccgcgc ctgatcgagg gcctcgtcga gccgctcggg cgacttccct ccggcggcgg
     2221 ccaggtccgc gcccttgccg ccgccgccgc cgacggtccg ggccgcctcg gcgatcagct
     2281 ccgcggcgtt cagcccgctg tcggagccga ccgcggcgac cagcgccacg cccttgccgc
     2341 cgggcgaggc gccgagcacg acagctcgga tcccgggacg gtcccgcaac gccaccgcca
     2401 ggtcccggac ctcgtcacgg ctctcggcgt cgacgcgggc gacgacgacc ccgtcgaccg
     2461 cggacccggc caggtcctcg gcctggttgc cggcgagttg gcgacgcagg accttgagct
     2521 cgtcccgggc ggccttcagc tcggtgaccc gcttctcgat cgcgccctgc aggtcgtcga
     2581 cggggactcc gagcaggtcc gcggcggcgc gcagccggtc ctcctcctgt cgcagacgct
     2641 cgatcggacc ggtgccggtg accgcctcga tgcggcggat gttggagccg atcgaggtct
     2701 cggagacgac cttgagcggt ccgatgtcgc cgagggcccg cacgtgggtc cctccgcaca
     2761 gctcggtgga gtgccgaccc gcctcgagca cccgcacgac gtcgccgtac ttgtcgccga
     2821 agaacatgag cgcgcccagc tcccgggcct cgtccatggt ggtctcgaag tggcggacgg
     2881 gggcgttgtc gaggatctcg gcgttggcca ggtcctcgat gcgacggatc tcctcgccgc
     2941 tgaccgctcc ggggtgggcg aagtcgaagc gcaggcggtc gggaccgacc caggagccct
     3001 gctgcttgac gtggtcgccg agcacctcgc gcagcgccca gtgcaggatg tgtgtcccgg
     3061 tgtggttgcg gcggatggca gcccggcggt cggtgtcgat ggcggccacg gcggcctgcc
     3121 cctcggtgac ggtgccgtcg atgacctcca cggtgtgtcg gtgcacgccg ggcagcacgt
     3181 aggtcgtgtc ggtcacccgc agggttccgg tggcggtcgt gatcgtgccg gtgtcgccga
     3241 cctgaccgcc cgactcggcg tagaacgggg tgcggtccaa cacgacggcg tcgtcgacga
     3301 cggccagcac ggtcgcgtcg ctgcggtcct cgtcacggcc ggtgaactcg gtcggtcccc
     3361 gctcggcgag cagccgccgg gcgacatcgg ccagctcgcc ggcgtcgacc gcaccacgct
     3421 tggaggaagt ccgggaacgc tcccgttggg cggccatgtc ggcgtcgaag ccggccaggt
     3481 ccaccgacac cccccgcagg tcggccatct cctgggtgac ctcgagcggg aagccgtagg
     3541 tgtcgtgcag tgcgaaggcc accgacccgt cgagctcgcc gcccgcaggg atctcgtcga
     3601 gcgccgagtc gagcagcacc gaaccccggg cgagggtgcg gcggaaaccc tcctcctcgc
     3661 gcgagatcgc ctcgacgatg cggccacgcc cctcgaccag gtccgggtac gcgccgccca
     3721 tgatgtcgat gcagcactcg accatgggcg ccatgaccag gtcgtcgacg tcgagcatgt
     3781 aggcgaagcg aaccgcccgg cggatgatgc ggcgcaggac gtaaccacgg tcctcgttgg
     3841 aggggacgac cccgtcggcg accaggaacg tggcggtccg ggtgtggtcg gccaacagcc
     3901 gcagggcgat ggcggacagc tcgccgtcgc cgaccgaacg accggtgacc tcctcggcac
     3961 gggtcaccag ccgggcgagg acgtcggcgg agtacagcga cgggctcccg gccaggaccg
     4021 cgaggatgcg ctccaacccg gcgccggtgt cgatgttgcg ggtggggagg tcggtcagct
     4081 cgccgtcggc gccccggaag tactgctgga acaccaggtt ccagatctcg acgtagcggt
     4141 cctcggcggc ggggttggcc ggcccgccgt cgggtcctgc ctccgggccg tagtcgaaga
     4201 acagctccga gctgggtccg caggggccgg tctcgcccat ctcccagaag ttgtccttgt
     4261 cgaggcgctg gatgcgctcg gccgggaagc cgacctggtc gatccagatg cgctcggcct
     4321 cgtcgtcgga gacgtgcacg gtcacccaga tccgctctcc gtcgatgccg aggacgtcgg
     4381 tgacgaactc ccaggcccat gcgatcgcct cggacttgaa gtagtcgccg aagctgaagt
     4441 tgccgagcat ctcgaagaac gacaggtggc gctgcgagcg gccgatggcg tcgaggtcgt
     4501 tgtgcttgcc cccggcccgc acgcacttct gcaccgagac cgcccgcggc ggccggtacg
     4561 gcaccggctc ctcgcccagg aacatgggca cgaacggcat catccccgag ttggtgaaca
     4621 tgggagcgga ggggtgcgtc gggatcagac cgcccgacgc gaccaccgtg tgggcccgct
     4681 cgttgaagaa ctcggtccag gcgcgacgca gccggtccgc ggtccagccc ccggcggaat
     4741 cggacggggg tcgggcgaca tcggcggagg tgtcgccgcg gggcgcggtc ggctcggtca
     4801 taggtccgct caggatatcg gtgcgaccac gccggcccgg ccgcagttcg gccctctgcg
     4861 gggcgggtgg ttcaccagcg ggcggggcgg g
//
LOCUS       DAOQOD010000213         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7294_length_4889_cov_11.250931, whole genome shotgun sequence.
ACCESSION   DAOQOD010000213 DAOQOD010000000
VERSION     DAOQOD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7294_length_4889_cov_11.250931"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLZ72_14515"
     CDS             <1..393
                     /locus_tag="PLZ72_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNA37558.1"
                     /translation="AVATTGETLGDAQGIPTKYLESILAELRRSGIIGSRRGSAGGYW
                     LARPASEVSIADIVRAVEGPLADVRGEAPEELSYDGPAQPLERVWIATRASVRRVLEA
                     TTLAHLVDGELPAEVTALLSDDQAWSRR"
     gene            complement(409..1872)
                     /locus_tag="PLZ72_14520"
     CDS             complement(409..1872)
                     /locus_tag="PLZ72_14520"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA37559.1"
                     /translation="MSPTPRPPDCVPDHLDLLIGGETTPAADGATFAVADPSTGATLA
                     TVARAGAADLERAIATARAAFDPGVWADVDATSRGRVLRRVAELIRERIEEFAWAEVA
                     GAGHTIEDARWEAGAMAEVFEYYAGAADKHLGAVVPTPDDGLGLVLRVPVGVCGLIVP
                     WNFPMLIATWKLAPALACGNAVILKPASLTPITALMLGTLLVEAGVPAPCVTVLPGPG
                     ATVGDALVADGRVDKISFTGDSSTGSHLLRTLSPNITRISLELGGKSAAIVFADADLD
                     SAIDAIPYSVFANAGQDCCARSRVLVQRSVYDDVVDRLVERTGRVVVGDPADTATEVG
                     PMISEAQRATSLEYLDIARSEGAEVLCGGDASGPGWFLTPAVVAGADNSMRVAREEIF
                     GPVMTVIPFDDEGDAVRIANDSDYGLSGTLWTRDVGRAVRVGRAVRTGVMSVNTNRSV
                     RYQLPFGGFRRSGMGRELGLAALDHYTESKTLFLSAD"
     gene            2008..2811
                     /locus_tag="PLZ72_14525"
     CDS             2008..2811
                     /locus_tag="PLZ72_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37560.1"
                     /translation="MAGRLDGKVALITGAAAGLGRVAAELFADEGADVVIADVTDGAE
                     AVEAIAAGGGRAAFVSTDVTDEESVRAAVEFATAEFGGLHVLYNNAGVSLADDDGVTN
                     TDASVWDTTLRVNVTGVALGCKHGIPAMLATVEADRQAGRRAGASIVNVASFVAHLGA
                     ATPQIAYTASKGAVLAMTREIAVIHARQGLRANALCPGPVLTPLLAKYLDDDAKRQRR
                     LVHIPMGRFGEPAEIAQGALFLASDESSFMTGQSLLVDGGITAAYTTPE"
     gene            2879..4297
                     /locus_tag="PLZ72_14530"
     CDS             2879..4297
                     /locus_tag="PLZ72_14530"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNA37561.1"
                     /translation="MSERASGLIDVATLARLVDDGTIDTVVVAFTDLQGRLMGKRVTG
                     RFFLDHVLGPDGSASDGEGIEACDYLLTVDVDMNVVEGYRFANWEAGYGDFRGRPDLS
                     TLRITPWLEGTALVLADVLDNADDSPVAVSPRQVLQHQIARAAERDLVVMIGSELELF
                     AYDAGYDELARNHYRDLGQRTSSWYNLDYHVLQTTKDEPLIRALRNGMVAAGLPVEFS
                     KGEAAPGQHELNLRYSDALTMADNHTVYKNGAKEIAHLQGKAITFMAKPRIDEPGSSC
                     HIHSSIWSPDGRPLAPGSGGDADRFGMSSAFRAWMGGIQAHSRELSLCFAPFVNSYKR
                     YQAGSWAPTAVVWSPDNRTCGLRLVGHGAAMRVESRIPGADCNPYIAFAAIIAAGLDG
                     IERGLDCGDPFVGNAYTAEDVERIPWNIVEAIDAFEQSDFARDALGDDVHFHLLHTAR
                     AEWRASNDVVTEWELRRNFERI"
     gene            complement(4324..>4889)
                     /locus_tag="PLZ72_14535"
     CDS             complement(4324..>4889)
                     /locus_tag="PLZ72_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNA37562.1"
                     /translation="LGMDETTFWVDGDDADRLAALYIPFGEGGRAVRWDEGGSAATRR
                     PEFLSGGGGLVSSAGDYHRFMEMLRRRGELDGVRILGPRTVDYMTSNHLPGGVDLEAF
                     GQSTWAETTFDGVGFGLGFSVLMDPAPYRTIGSAGAYGWGGAASTAFSIDPVEDLTLA
                     FYTQLLPSSTYPIRTQLQQLVHQAIVD"
BASE COUNT          721 a         1764 c         1754 g          650 t
ORIGIN      
        1 gcggtcgcga cgacgggcga gaccctggga gacgcgcagg gcatcccgac caagtacctc
       61 gagagcatcc tggccgagct ccgccgttcg gggatcatcg gcagccgccg agggtccgcc
      121 gggggctact ggctggcccg accggcctcc gaggtgtcca tcgccgacat cgtccgggcc
      181 gtcgaggggc cgctggccga cgtgcgcggc gaggcacccg aggagctgtc ctacgacggc
      241 cccgcccaac cgctggaacg ggtgtggatc gccacccggg ccagcgtgcg gcgcgtcctg
      301 gaggcgacca cgttggcgca cctggtcgac ggcgagctcc cggcggaggt gacggccctg
      361 ctgtccgacg accaggcgtg gagccgccgc tgatcgcggc cggatgcgtc agtccgcgga
      421 caggaacagg gtcttggatt cggtgtagtg gtcgagcgcg gccaggccca gctcgcggcc
      481 catgccggag cgcctgaaac cgccgaaggg cagctggtag cgcacgctgc ggttggtgtt
      541 gacgctcatc accccggtgc gaaccgcccg gcccacgcgc acggcccggc cgacgtcgcg
      601 ggtccacagg gtccccgaca ggccgtagtc gctgtcgttg gcgatgcgca cggcgtcgcc
      661 ctcgtcgtcg aaggggatga ccgtcatcac cggcccgaag atctcctcgc gggcgacgcg
      721 catcgagttg tcggccccgg ccaccaccgc cggggtcagg aaccagcccg gcccggacgc
      781 gtcgccgccg cagagcacct cggcgccctc ggagcgggcg atgtcgaggt actccaggct
      841 ggtggcccgc tgtgcctccg agatcatcgg accgacctcg gtcgcggtgt cggcggggtc
      901 gccgacgacc acccgaccgg tccgctcgac gagtcggtcg accacgtcgt cgtagaccga
      961 ccgctggacc aggacccggc tgcgggcgca gcagtcctgg ccggcgttgg cgaacaccga
     1021 gtacgggatc gcgtcgatcg ccgagtcgag gtcggcgtcc gcgaacacga tcgccgcgga
     1081 cttgccgccc agctccaacg agatccgtgt gatgttcggc gacagcgtgc gcaggaggtg
     1141 cgacccggtc gacgagtcgc cggtgaagga gatcttgtcg acccggccgt cggccaccag
     1201 cgcgtcgccg acggtggcgc cgggacccgg cagcacggtg acgcagggtg cggggacccc
     1261 ggcctcgacc aggagggtgc cgagcatgag cgcggtgatc ggcgtcagcg acgccggctt
     1321 gaggatgacc gcgttgccgc aggccagtgc gggggcgagc ttccacgtgg cgatcagcat
     1381 cgggaagttc cacggcacga tcagtccgca caccccgacc gggacccgca gcaccagccc
     1441 cagcccgtcg tcgggggtgg gcaccacggc gcccaggtgc ttgtcggccg cgccggcgta
     1501 gtactcgaac acctcggcca tcgcgcccgc ctcccagcgg gcgtcctcga tcgtgtgccc
     1561 ggcgccggcc acctcggccc acgcgaactc ctcgatccgc tcccggatca gctcggcgac
     1621 ccggcgcagc acccggcccc gcgaggtggc gtccacgtcg gcccacacac cggggtcgaa
     1681 cgcggcgcgg gctgtcgcga tcgcacgctc gaggtcggcg gcgccggcgc gggcgacggt
     1741 ggccagcgtg gcaccggtcg acgggtcggc gacggcgaac gtggcgccgt cggcggcggg
     1801 cgtggtctcg ccgccgatca gcaggtcgag gtgatcgggg acgcaatccg gcggacgggg
     1861 agtcggactc atggaggcga gtgtaggaac ccggggtggc gggcccccat cccgggctcg
     1921 ctaacgtgcc gccgggccgc agacctcgcc accgcgctgg ggcacggccg gtccctcctc
     1981 ccccacccac gacaggaaga tcccaccatg gcaggacgac tcgacggcaa ggtggcgctc
     2041 atcaccggag ccgccgcggg actgggtcgc gtggcggccg agctgttcgc ggacgaaggg
     2101 gccgacgtcg tcatcgcgga cgtcaccgac ggcgcggagg ccgtggaggc catcgccgcg
     2161 ggcggcgggc gtgccgcgtt cgtgtcgacc gacgtgaccg acgaggagtc ggtgcgcgcc
     2221 gcggtcgagt tcgccaccgc cgagttcggt gggctgcacg tcctctacaa caacgccggg
     2281 gtgtcgctgg ccgacgacga cggggtcacc aacaccgatg cgtcggtgtg ggacacgacg
     2341 ctgcgcgtca acgtcacggg cgtggcgctc ggctgcaagc acgggatccc cgccatgctc
     2401 gcgaccgtcg aggccgatcg gcaggcgggg cgtcgcgccg gggcgtcgat cgtcaacgtg
     2461 gccagcttcg tcgcccacct cggcgccgcc accccccaga tcgcctacac cgcgtccaag
     2521 ggggcggtgc tcgccatgac gcgggagatc gcggtgatcc acgcccgcca ggggctgcgg
     2581 gccaacgccc tgtgccccgg tccggtgctc accccgctgt tggccaagta cctcgacgac
     2641 gacgccaagc ggcagcggcg gctggtgcac atcccgatgg ggcggttcgg cgaaccggcc
     2701 gagatcgccc agggcgccct cttcctcgcc tccgacgagt ccagcttcat gaccggccag
     2761 tcgctgctgg tcgacggcgg catcaccgcg gcgtacacca cgcccgagta gtcgcccgac
     2821 agggggcggc gtcgccggac cggggacgga ggccgtcggc gtcgtaccat cggcgaccat
     2881 gtccgaacgc gcctcaggtc tgatcgacgt agcgaccctc gcccggctgg tcgacgacgg
     2941 caccatcgac accgtcgtcg tcgccttcac cgacctccag ggccggctga tgggcaagcg
     3001 ggtgaccggt cggttcttcc tcgaccacgt cctcgggccg gacggctcgg cctccgacgg
     3061 tgagggcatc gaagcgtgcg actacctgct gaccgtcgac gtcgacatga acgtggtcga
     3121 gggataccgg ttcgccaact gggaggccgg ctacggcgac ttccggggcc gacccgacct
     3181 ctcgaccctg cgcatcacgc cgtggctcga gggcacggcg ctggtgctcg ccgacgtgct
     3241 cgacaacgcg gacgactccc ccgtcgcggt gtcgccccgt caggtccttc aacaccagat
     3301 cgcccgcgcc gccgagcgcg atctggtggt catgatcggt tccgagctcg agctgttcgc
     3361 ctacgacgcc ggctacgacg agctcgcccg caaccactac cgcgacctcg gacagcggac
     3421 gagcagctgg tacaacctcg actaccacgt gctgcagacc accaaggacg agccgctgat
     3481 ccgtgccctg cgcaacggga tggtagccgc gggtctgccg gtcgagttct ccaaggggga
     3541 ggccgctccc ggccagcacg aactcaacct ccggtactcc gacgcgctga ccatggccga
     3601 caaccacacc gtctacaaga acggcgccaa ggagatcgca cacctccagg gcaaggcgat
     3661 cacgttcatg gccaagcccc gcatcgacga gccgggatcg agctgccaca tccactcgtc
     3721 gatctggagc cccgacggtc gaccgctcgc ccccggctca gggggcgacg cggaccggtt
     3781 cgggatgtcg tccgcgttcc gggcctggat gggaggaatc caggcccact cccgcgagct
     3841 gtcgctgtgc ttcgcgccgt tcgtcaactc ctacaagcgg taccaggcgg ggtcgtgggc
     3901 cccgaccgcg gtcgtgtggt cgcccgacaa ccggacgtgc ggtctgcgcc tggtcggcca
     3961 cggggctgcg atgcgcgtcg agtcccgcat cccgggtgct gactgcaacc cctacatcgc
     4021 cttcgccgcg atcatcgccg ccggactcga cggtatcgag cgcggcctcg actgcggtga
     4081 cccgttcgtc ggcaacgcct acaccgccga ggacgtggag cggatcccgt ggaacatcgt
     4141 cgaggcgatc gacgcgttcg agcagtccga cttcgctcgg gacgcgctcg gtgacgacgt
     4201 gcacttccac ctgctgcaca ccgcccgcgc cgagtggcgg gccagcaacg acgtggtcac
     4261 cgagtgggag ctgcgccgga acttcgagcg catctgagcg gggacggggt cccggccgag
     4321 tcgtcagtcg acgatcgcct ggtgcaccag ctgctgcagc tgggtgcgga tcgggtaggt
     4381 gctcgacggc agcagctgcg tgtagaaggc cagggtcagg tcctcgaccg ggtcgatgct
     4441 gaacgcggtc gacgccgccc cgccccagcc gtaggcgccc gcggagccga tcgtccggta
     4501 cggcgccggg tccatgagga cgctgaagcc caggccgaag ccgacgccgt cgaaggtggt
     4561 ctcggcccag gtggactgac cgaacgcctc caggtccacg ccgccgggca ggtggttgga
     4621 ggtcatgtag tccaccgtgc gcggcccgag gatgcggacg ccgtcgagct cccctctgcg
     4681 gcgcagcatc tccatgaacc ggtggtagtc cccggcgctc gacaccagtc cgccgccgcc
     4741 ggagaggaac tcggggcgac gggtggcggc ggagccgccc tcgtcccaac gcacggcgcg
     4801 gccaccctcg ccgaacggga tgtagagggc ggcgaggcgg tcggcgtcgt cgccgtcgac
     4861 ccagaaggtc gtctcgtcca tgcccaggg
//
LOCUS       DAOQOD010000214         4874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7330_length_4874_cov_10.894999, whole genome shotgun sequence.
ACCESSION   DAOQOD010000214 DAOQOD010000000
VERSION     DAOQOD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7330_length_4874_cov_10.894999"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..989)
                     /locus_tag="PLZ72_14540"
     CDS             complement(81..989)
                     /locus_tag="PLZ72_14540"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37563.1"
                     /translation="MGICDGRVVIVTGAGRGLGRAHALAFAAEGARVVVNDLGVAQDG
                     SDPSDTPAQQVVDEILAAGGEAVASYHDVADWDTAGEMVQLAIDTFGGLDVLVCNAGI
                     VRDRMFVNSEQSEWEAVLRVHVLGHAAPARHAATYWRARSKAGEPVDARIINTSSGAG
                     LMGSIAQAAYSAAKGAITSMTLVQAAELGRIGVTANALAPAARTRMTEEAFADMMAEV
                     DEGAFDAMAPENVSPLVVWLGSAESAGVTGRVFEVEGGIIGIAEGFRHGPRFDKGARW
                     EPAEIGPVVADLLAEATAPTPVYGAG"
     gene            1125..1967
                     /locus_tag="PLZ72_14545"
     CDS             1125..1967
                     /locus_tag="PLZ72_14545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNA37564.1"
                     /translation="MQFGVTMFATDVSMPVPDLAVAAEERGFDSVWIPEHTHIPTSRR
                     TPPPTGDAELPEEYKRSVDPLVALAAAAALTTTLRLGTGVMLPMQREPIVTAKAVATL
                     QNLSGGRFDLGIGFGWNEDEMADHGVQYATRRDHTRDHVRAMQALWTDDVASFDGEFV
                     SFPPTWSWPKPEVRVPVLIGGGAGPKMFAHLAEYADGWIPIGGAGLSESIPRYKEAVA
                     AAGRDPEAMRIIPFGSLPTAPKLEHFASIGVTECVFRLPSAPTDEVMTVLDQQAELLA
                     ELRG"
     gene            2000..2476
                     /locus_tag="PLZ72_14550"
     CDS             2000..2476
                     /locus_tag="PLZ72_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNA37565.1"
                     /translation="MAGPNDMTTYPEDELRRFYARHRALHEAGDWQGLGDLFTDDASY
                     LDSVYGWSHGLPAIRSFLERSMTGLEDWSFPISAVAYDADAGVILTHWSNRLPGTRAD
                     GTHYEVPGASVLTYAGDGRISRQMDLFDTAWMIREIKAWIADHDGALPYPDPGVRT"
     gene            2495..2752
                     /locus_tag="PLZ72_14555"
     CDS             2495..2752
                     /locus_tag="PLZ72_14555"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HNA37566.1"
                     /translation="MTATSGGRPAPKQGFRPSGRQIVGAVVAVVLLLFIVTNTETAPV
                     TLLLWTFEMPLWLALAIAAAMGVLIGMGLGARRTKRKYMDV"
     gene            complement(2753..3562)
                     /locus_tag="PLZ72_14560"
     CDS             complement(2753..3562)
                     /locus_tag="PLZ72_14560"
                     /inference="COORDINATES: protein motif:HMM:NF005909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37567.1"
                     /translation="MMLEGLTAVVTGVGPGMGRDIALGLAREGADVALVARSERNVPA
                     VAAEVEALGRRAVPVYGNIADGDDCARIAAEVRAAFGDQLDILVNSAFYGGLDERFED
                     ADLDKWQRPIKVNYLGTLRLTQAMLPQLKAAGAAKGDARIVMVNTMSVQVLEEGAGAY
                     AGSKAALGAVSKTLARELGGYGIRVNSVHPGYIWGDSVKIYLEWQAQERGGDATWETV
                     YEELAAETALGYLPHSSEIAGAVVFYASPLARCVTGTALPVNGGHWMPPSA"
     gene            complement(3589..>4874)
                     /locus_tag="PLZ72_14565"
     CDS             complement(3589..>4874)
                     /locus_tag="PLZ72_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37568.1"
                     /translation="TRGWNAFQFRGLPSAPVDFDDSPALAEFRAEVRAFLDEHAELRH
                     GDERDWSRNGATTDEEVAADYRARCRRWQATLADHGWAGLTWPAAFGGRGGTPAEQIV
                     FNQELARYDATSGFITAAQALVGPTLMKHGSAAQQERYLSPLLRGDEWWCQLFSEPGA
                     GSDLSSMATRAVLDEATGEWVVNGQKVWTSNAQYADFGILVARTDPDVPKHAGLTYFI
                     VDMRSPGIEIRPLVQAQGVAHFNEVFLSDVRIPADNVVGEVGDGWNVTRTTLRSESSM
                     ISGAGQATPFTAVLALARRHGRTDDPTVRQDLARVYANEQIQKWMGWRTQTAVMTGRY
                     ELMLHGSLLKNFFTRSFAFKVGLALDLEGAEGMLSDDAEADGFWQYQFLNQFASRIGG
                     GTEEVHRNNLGEQALGLPREPSVDRDVPWNQTRRS"
BASE COUNT          706 a         1781 c         1688 g          699 t
ORIGIN      
        1 cgcgatcatg accgtcctgc tcggggtgat cggcgccaag gtgctgggcc agggcatcgg
       61 cggcgcctgg ggttgagcgg tcagcccgcg ccgtagaccg gggtgggtgc ggttgcctcg
      121 gccagcaggt cggcgacgac cggaccgatc tcggccggct cccaacgggc gcccttgtcg
      181 aagcggggac cgtggcggaa cccctcggcg atgccgatga tgccgccctc gacctcgaac
      241 acccgaccgg tcaccccggc cgactccgcc gaaccgagcc acacgaccag gggcgacacg
      301 ttctcgggcg ccatggcgtc gaaggctccc tcgtcgacct cggccatcat gtcggcgaac
      361 gcctcctcgg tcatccgggt gcgcgccgcg ggggccaggg cgttggcggt gacgccgatg
      421 cgaccgagct cggcggcctg caccagggtc atgctggtga tcgccccctt ggccgccgag
      481 taggcggcct gggcgatcga gcccatcaga cccgctcccg acgacgtgtt gatgatgcgg
      541 gcgtcgaccg gctcccccgc cttggaacgg gcccgccagt aggtggcggc gtgtcgggcc
      601 ggagcggcgt ggccgagcac gtggacccgc agcaccgcct cccactcgga ctgctcggag
      661 ttgacgaaca tgcggtcccg cacgatgccg gcgttgcaga ccagcacgtc gagtccgccg
      721 aacgtgtcga tcgccagctg caccatctcg ccggccgtgt cccagtcggc gacgtcgtgg
      781 taggaggcga ccgcctcacc acccgcggcg aggatctcgt cgaccacctg ctgggccggg
      841 gtgtcggacg ggtccgaccc gtcctgggcc acccccaggt cgttgacgac gacccgggcg
      901 ccctcggcgg cgaaggccag ggcgtgggcc cggccgaggc cccgtcccgc tccggtcacg
      961 atcacgactc gtccgtcaca gattcccatg ggcacagaat ctgacacccc gtcacatgcg
     1021 acacaaaccc ggtacgctcc gacacgggcg ccgcgggggt gcggtgccgt ccgctccggg
     1081 gtggcgctca cgccgcccac ctcgcacacc agggggttcc gccggtgcag ttcggcgtca
     1141 cgatgttcgc caccgacgtc tcgatgccgg tgcccgacct ggccgtcgcg gccgaggagc
     1201 gcgggttcga ctcggtgtgg atccccgagc acacccacat cccgaccagt cgtcggaccc
     1261 caccgcccac cggcgacgcc gagctgcccg aggagtacaa acgctcggtc gacccgctcg
     1321 tcgccctggc cgccgcggcg gcgctcacca ccaccctccg cctcggcacc ggcgtcatgt
     1381 tgcccatgca gcgcgagccg atcgtgaccg ccaaggccgt cgccaccctg cagaacctgt
     1441 ccgggggcag gttcgacctg gggatcggct tcggctggaa cgaggacgag atggccgacc
     1501 acggcgtgca gtacgccacc cgacgcgacc acacccgcga ccacgtccgg gccatgcagg
     1561 ccctgtggac cgacgacgtc gcctccttcg acggcgagtt cgtgtcgttc ccaccgacct
     1621 ggtcctggcc caagcccgag gtgcgcgtcc cggtgctgat cggcggaggt gccggaccga
     1681 agatgttcgc ccacctcgcc gagtacgccg acggttggat ccccatcggc ggcgccgggc
     1741 tctccgagtc gatcccccgc tacaaggagg ccgtcgccgc ggcggggcgt gaccccgagg
     1801 ccatgcggat catcccgttc ggctcgctgc cgaccgcacc caagctggag cacttcgcct
     1861 cgatcggcgt caccgagtgc gtgttccggc tgccgtccgc tccgaccgac gaggtgatga
     1921 cggtcctcga ccagcaggcc gagctgctgg ccgagctgcg gggttgaccc cgtcacgaac
     1981 accacgagga ggacctcgca tggccggacc gaacgacatg acgacgtacc ccgaggacga
     2041 gctgcggcgc ttctacgccc gccatcgcgc cctgcacgag gccggggact ggcagggtct
     2101 cggcgacctg ttcaccgacg acgccagcta cctcgactcg gtgtacggct ggagccacgg
     2161 actgccggcc atccgatcgt tcctggagcg gtcgatgacc gggctcgagg actggtcctt
     2221 cccgatctcg gcggtcgcct acgacgcgga tgccggcgtg atcctgaccc actggagcaa
     2281 ccggctgccc ggcacccggg cggacggcac ccactacgag gtgccgggcg cgtcggtgct
     2341 cacctacgcc ggcgacgggc ggatcagccg acagatggac ctgttcgaca ccgcctggat
     2401 gattcgggag atcaaggcct ggatcgccga tcacgacggg gcgctcccct accctgaccc
     2461 cggagtcagg acctgaccca cacggaggtg gaccatgacg gcgacgagcg gcggacgacc
     2521 ggcacccaag caggggttcc ggccctcggg ccgtcagatc gtcggcgccg tcgtcgcggt
     2581 ggtgctgctg ctgttcatcg tcaccaacac cgagaccgcc ccggtgaccc tgctgctgtg
     2641 gaccttcgag atgccgctgt ggctggccct cgccatcgcc gcggcgatgg gcgtcctgat
     2701 cgggatggga ctcggcgccc ggcggaccaa acgcaagtac atggacgtct gatcaggccg
     2761 acggcggcat ccagtgcccg ccgttgaccg gcagcgcggt cccggtgacg cagcgggcca
     2821 gcggcgacgc gtagaacacg accgccccgg cgatctcgga gctgtggggc aggtacccga
     2881 gagcggtctc cgccgcgagc tcctcgtaga ccgtctccca ggtggcgtct ccgccgcgct
     2941 cctgggcctg ccactcgagg tagatcttca cgctgtcacc ccagatgtag cccgggtgaa
     3001 ccgagttgac ccggatcccg tacccgccga gctcccgggc cagggtcttg gacaccgcgc
     3061 cgagcgcggc cttggacccg gcataggcac cggcgccctc ctcgaggacc tggaccgaca
     3121 tggtgttgac catgacgatg cgggcgtcgc ccttggcggc accggccgcc ttcaactgcg
     3181 gcagcatcgc ctgggtcaac cgcagggtgc ccaggtagtt gaccttgatc ggccgctgcc
     3241 acttgtcgag gtccgcgtcc tcgaagcgtt cgtcgaggcc gccgtagaac gccgagttca
     3301 ccaggatgtc gagctggtcg ccgaacgcgg cgcggacctc agccgcgatc cgggcgcagt
     3361 cgtcgccgtc ggcgatgttg ccgtacaccg gcacggcgcg ccgaccgagc gcctcgacct
     3421 cggccgcgac ggccggcacg ttgcgctccg aacgggccac cagggcgacg tccgcgccct
     3481 cgcgggccag gccgagagcg atgtcacgcc ccatgcccgg ccccactccg gtgaccaccg
     3541 cggtcagtcc ttcgagcatc atcgtcggtt ccccctgtga cggcccggtc aggaacgccg
     3601 ggtctggttc cacggcacgt cgcggtccac cgacggctca cgcggcaggc cgagcgcctg
     3661 ctccccgagg ttgttgcggt ggacctcctc ggtgccaccg ccgatgcgcg aggcgaactg
     3721 gttgaggaac tggtactgcc agaacccgtc ggcctcggcg tcgtcggaca gcatcccctc
     3781 ggcgccctcg aggtcgagcg ccagtccgac cttgaacgcg aagctgcggg tgaagaagtt
     3841 cttgagcagc gacccgtgca gcatcagctc gtagcgtccg gtcatcaccg cggtctgcgt
     3901 gcgccagccc atccacttct ggatctgctc gttggcgtac acccgggcca ggtcctgacg
     3961 cacggtcggg tcgtcggtcc ggccgtggcg gcgggccaac gccaggaccg cggtgaacgg
     4021 ggtggcctga ccggcccccg agatcatcga cgactcggag cgcagcgtgg tgcgggtgac
     4081 gttccagccg tcgcccacct cgcccacgac gttgtcggcg gggatgcgca cgtcggacag
     4141 gaacacctcg ttgaagtgcg ccacgccctg ggcctgcacc agcggacgga tctcgatgcc
     4201 cggcgaacgc atgtcgacga tgaagtacgt cagtccggcg tgcttgggga cgtcgggatc
     4261 ggtccgggcg acgaggatgc cgaagtccgc gtactgggcg ttggaggtcc agaccttctg
     4321 gccgttgacg acccattcgc cggtcgcctc gtcgagcacc gcccgcgtcg ccatcgacga
     4381 gaggtccgag cccgcccccg gctcggagaa cagctggcac caccactcgt cgccccgcag
     4441 caacggcgaa aggtagcgct cctgttgcgc cgcgctgccg tgcttcatga gcgtcgggcc
     4501 gaccagggcc tgggcggcgg tgatgaagcc cgaggtggcg tcgtagcggg cgagctcctg
     4561 gttgaagacg atctgctcgg ccggggtccc accccgaccg ccgaacgcgg cgggccaggt
     4621 caggccggcc cagccgtggt cggcgagggt cgcctgccag cgacggcaac gggcccggta
     4681 gtcggccgcg acctcttcgt cggtggtggc gccgttgcgg gaccagtccc gttcgtcgcc
     4741 gtggcgcagc tcggcgtgct cgtccaggaa tgcccgcacc tcggcgcgga actcggcgag
     4801 ggcgggggag tcgtcgaagt ccacgggcgc cgatggtagg ccgcggaact ggaacgcgtt
     4861 ccacccgcgg gtcg
//
LOCUS       DAOQOD010000215         4768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7589_length_4768_cov_10.589646, whole genome shotgun sequence.
ACCESSION   DAOQOD010000215 DAOQOD010000000
VERSION     DAOQOD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7589_length_4768_cov_10.589646"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..517
                     /locus_tag="PLZ72_14570"
     CDS             122..517
                     /locus_tag="PLZ72_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37569.1"
                     /translation="MDPTEVDVSDCCVKHPMNIAEFACSSCGHDFCPECVVFPFGLRK
                     PPLCLACALAKGGISRRDQGLPKLGRRQVKQRVKARRQTTVTRSEMHSSGLGQAVETV
                     PSVEEQQAASLLDGDRSPDEFPGGWKQVF"
     gene            complement(587..>1063)
                     /gene="purE"
                     /locus_tag="PLZ72_14575"
     CDS             complement(587..>1063)
                     /gene="purE"
                     /locus_tag="PLZ72_14575"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNA37570.1"
                     /translation="LVGVIMGSSSDLEVMTPAVDVLAEFGVIHEVHVVSAHRSPMRMV
                     DYAQQAAGRGLRVIIAGAGGAAHLPGMVAAMTVLPVIGVPVPTRHLGGADSLHSIVQM
                     PSGVPVATMAIGGSANAGLLAVRILALSDDDLRQRLEEHQRGLERHALDQDAGLPR"
     assembly_gap    1064..1163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1164..>2244)
                     /locus_tag="PLZ72_14580"
     CDS             complement(<1164..>2244)
                     /locus_tag="PLZ72_14580"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNA37571.1"
                     /translation="EPVVGVIGGGQLARMLAEAASPLGVHLRVLASPSDEGAAAVVAD
                     TVLGDHSDAATLDAFADSVDVITFDHENVDHGLLSALEARGVAVRPSVATLLYSDKAH
                     QRRRFAEAGIPVPEFLVLDPTGDPDRPAGAATCAADAVAFAERHGGVAVVKASRGGYD
                     GRGVWMLPASDIGAFVETWTGAPLVLEPRLELSLELAVLAARTPSGEVATWPLLETVQ
                     VDGMCDEVLLPAAVPAELEVRARALGARVAEEVGAQGVIAVELFVVSGSDEPDDAHGA
                     DATGPRLFVNEIAPRVHNSGHLTIEASATSQFEQHLRAVLDWPLGPTHSVSPGAVMAN
                     VVGTDAQEPRRRQARALAEVPGAHVH"
     assembly_gap    2245..2344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2345..3129)
                     /locus_tag="PLZ72_14585"
     CDS             complement(<2345..3129)
                     /locus_tag="PLZ72_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNA37572.1"
                     /translation="MKVCVIGSGGREHALADVLGRDAEVVVTPGNPGIPGSVATPPEE
                     LEADLFVVGPEVPLVDGLADRLRASGRRVFGPGADGARLEGSKAWMKELVAAAGVPTA
                     RHGTFDSVEPAHAMLDTLTPPFVIKTDGLAAGKGVVVTESLAEARNAVGDYLSGVSFG
                     DAGRTLVIEEGLSGPELSVLAVCDGERAVLLPPAQDFKRIGDGDSGPNTGGMGAYSPV
                     PIVTDDVLSTVMDRFVGPTLAELRRRGIDYRGVLYAGLMFTADG"
     gene            complement(3140..4000)
                     /locus_tag="PLZ72_14590"
     CDS             complement(3140..4000)
                     /locus_tag="PLZ72_14590"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNA37573.1"
                     /translation="MGDLDLDTTLSPLDGARADGVVRFGVDLSDDWRIWTLNGGYVAA
                     VALRAAGAVTSFDRPASISLQLLSGAVAGPAEVSVRSARRARSAECLAVVIDQGGRRI
                     AEGHVWSVASVADGPSHDDAGDRPAGPPLSYPTIHEHIAARGSDAPAAPDFPFWGNFE
                     SRPISWVEDWDDRPAGPAFAGDWLRFAPTATFADPFLDAGRVAVALDVYSFPSLVRAH
                     EPPIGWTAPNIDLHCSFHRRSDDSPWILGVGRTPVAEDGLAGFTAEVWSEDGRLLASA
                     GGQMLVRAVG"
     gene            complement(4064..>4768)
                     /locus_tag="PLZ72_14595"
     CDS             complement(4064..>4768)
                     /locus_tag="PLZ72_14595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNA37574.1"
                     /translation="VAPRVAPYVADTVSLVHESLERGEHVLFEGAQATFLDLDHGTYP
                     FVTSSNPIAGGACVGAGVGPRHIDRVIGIAKAYVTRVGSGPMPTELFDETGDRIVDLG
                     HEYGVNTKRRRRPGWFDAVMLRHAVRLNSLSEVAITKLDIFDTFETIKVCVAYEVDGV
                     RHDHLPYHQSDLHAATPVYEELPGWQQDLTGVRERHDLPANAVSYLEFLQDQIGVPIG
                     VVGTGPGRDQHVIFSG"
BASE COUNT          676 a         1644 c         1673 g          575 t
ORIGIN      
        1 cgcctcatca tcgcccaccg cgcacccccg gcgacaacgc acccccaccg ttctgggggg
       61 tcggagggcg ggatgttcaa gggcgggggc ggaacggccg atccccatcg ggaccttccg
      121 gatggaccca accgaggtag acgtgtccga ctgctgcgtg aagcacccca tgaacatcgc
      181 cgagttcgcg tgcagctcgt gcggccacga cttctgcccc gagtgcgtcg tgttcccctt
      241 cggcctgcgc aagccgccgt tgtgcctggc ctgcgcgctg gccaagggcg gaatcagccg
      301 ccgcgatcag gggctcccca agttgggacg tcgccaggtc aagcagcggg tcaaggcccg
      361 ccgccagacg acggtcaccc gctccgagat gcactcatcc ggtctgggcc aggccgtcga
      421 gacggtcccg agcgtcgagg agcaacaggc ggccagcctg ctcgacggcg accgcagccc
      481 cgatgagttc cccggcggct ggaagcaggt gttctgaccc cccaccccct cgctctgtcc
      541 cgcacagacg cggtgatcac cgcgtctgtg cgggacaaag cggtggtcag cggggcagcc
      601 cggcgtcctg gtcgagggcg tgacgctcca acccgcgctg gtgctcctcg aggcgctgcc
      661 gcagatcgtc gtcggacagc gccaggatgc gcactgcgag cagccccgcg ttggcggacc
      721 cgccgatggc catggtcgcg accggaacgc ccgacggcat ctgcacgatc gagtgcagcg
      781 agtcggcccc gcccaggtgg cgtgtcggga ccgggacgcc gatcactggc agcacggtca
      841 tcgcggccac catcccgggc aggtgcgccg ctcccccggc gccggcgatg atcacccgca
      901 gtccccgacc cgcggcctgc tgggcgtagt cgaccatgcg catcgggctg cggtgggccg
      961 acaccacgtg cacctcgtgg atgacaccga actcggccag gacatcgacc gcgggcgtca
     1021 tgacctcgag gtccgacgag gaccccatga tcactccgac gagnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnngtgcacg tgcgccccgg ggacctcggc gagcgcccgg
     1201 gcctgacgac ggcggggttc ctgcgcgtcg gtgccgacga cgttcgccat caccgcgccg
     1261 ggtgagaccg agtgcgtcgg acccaacggc cagtcgagca ccgctcgcag gtgctgctcg
     1321 aactgcgagg tggcgctggc ctcgatcgtg aggtgacccg agttgtggac gcggggcgcg
     1381 atctcgttga cgaagagcct gggcccggtc gcgtcagctc catgggcatc atcgggctcg
     1441 tcggacccgg aaacgacgaa cagctcgacg gcgatgacgc cctgagcccc cacctcttcg
     1501 gcgacccggg ctccgagcgc ccgcgcccgc acctcgagct cggccggcac cgcggcgggc
     1561 agcagcacct cgtcgcacat gccgtcgacc tgcaccgtct cgaggagcgg ccaggtggcg
     1621 acctctcccg acggcgtgcg ggccgccagg actgcgagct cgaggctcag ctcgaggcgg
     1681 ggctcgagca ccagcggcgc tccggtccag gtctcgacga acgcgccgat gtccgacgcc
     1741 gggagcatcc acacgccccg accgtcgtag ccgccgcggc tggccttcac gaccgcgaca
     1801 ccgccgtggc gttcggcgaa cgcgacggcg tcggccgcgc acgtcgccgc accggcggga
     1861 cggtcgggat cgccggtcgg gtcgagcacc aggaactccg gcacggggat acccgcctcg
     1921 gcgaagcgcc gacgttggtg ggccttgtcg gagtacagca gcgtggcgac cgacggccgc
     1981 acggccacgc cccgggcctc cagagcggag agcaggccgt gatcgacgtt ctcgtggtcg
     2041 aaggtgatca cgtcgaccga gtccgcgaac gcgtcgaggg tcgcggcgtc ggagtggtcg
     2101 ccgaggacgg tgtcggcgac cacggcggcc gcaccctcgt cggacggtga tgccaggaca
     2161 cggaggtgca cgccgagcgg cgacgccgcc tcggccagca tgcgggcgag ttggcctccc
     2221 ccgatgaccc cgacgacggg ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnccgtcc gcggtgaaca tgagcccggc gtagaggacg ccgcggtagt cgatgccccg
     2401 gcgccgcagc tcggcgagcg tcgggccgac gaagcggtcc atcaccgtgg agaggacgtc
     2461 gtcggtgacg atggggaccg gcgagtaggc acccatccca ccggtgttgg ggccggagtc
     2521 gccgtcgccg atccgcttga agtcctgtgc cgggggcagc agcaccgccc gctcgccgtc
     2581 gcacaccgcc aggacgctca gctccgggcc gctgaggccc tcctcgatca cgagcgtgcg
     2641 cccggcgtcg ccgaaggaca ccccggacag gtagtcgccc accgcgttgc gggcctcggc
     2701 gagcgactcg gtgaccacga cgcccttgcc ggcggccagg ccgtcggtct tgatcacgaa
     2761 cggcggggtg agcgtgtcga gcatggcgtg ggcaggctcg accgagtcga aggtcccgtg
     2821 gcgggcggtg gggacgccgg ccgcggcgac cagctccttc atccacgcct tggacccctc
     2881 gagccgggcc ccgtcggccc cggggccgaa gacccgccgg cccgacgccc gcaggcggtc
     2941 ggccaggccg tcgaccagcg gcacctcggg cccgaccacg aacagatcgg cctcgagctc
     3001 ctcgggcggc gtcgcgaccg agccggggat gccggggttg ccgggggtca ccaccacctc
     3061 ggcgtcccgt ccgagcacgt cggccagcgc gtgctcccgt cctccgctgc cgatgacgca
     3121 gaccttcacg gcggcagttc tagccgacgg cccggaccag catctgcccg ccggcgctgg
     3181 cgagcaggcg accgtcctcg gaccacacct cggcggtgaa cccggccagg ccgtcctcgg
     3241 cgaccggcgt gcggccgact cccaggatcc acggcgagtc gtcggagcga cgatggaacg
     3301 agcagtgcag gtcgatgttg ggcgccgtcc aaccgatcgg cggctcgtgc gctcgcacca
     3361 gcgacgggaa cgagtagacg tccagagcga cggcgacgcg ccctgcgtcg aggaacggat
     3421 cggcgaaggt cgcggtcgga gcgaagcgga gccagtcgcc ggcgaaggcg gggcctgccg
     3481 gccggtcgtc ccagtcctcg acccacgaga tcggccgcga ctcgaagttg ccccagaacg
     3541 ggaagtccgg cgccgccggg gcgtcggatc cccgagcggc gatgtgctcg tggatcgtcg
     3601 ggtaggacag cggcgggccg gccggccgat cgccggcgtc gtcgtgggac ggcccgtcgg
     3661 cgaccgacgc gacgctccac acgtggccct cggcgatgcg gcggcctccc tggtcgatga
     3721 cgacggccag gcactccgcg ctgcgggcgc gacgagcgct gcgcaccgac acctcggccg
     3781 gaccggcgac ggcgccgctc agcagctgca gcgagatcga ggcgggtcgg tcgaaggagg
     3841 tgacggcgcc ggcggcccgc agcgccaccg cggcgacgta gccgccgttc agggtccaga
     3901 tccgccagtc gtcggagagg tcgaccccga agcggacgac tccgtcggct cgggcgccgt
     3961 cgagcggcga cagcgtggtg tccagatcca ggtcacccac ccgagggtcc cccctcggga
     4021 cgcgcgccgg gccggagaaa gcaggtcgtc cggggtcggg aactcatccg gagaagatga
     4081 cgtgctggtc gcgccccgga ccggtgccga ccacgccgat gggcacgccg atctgatcct
     4141 gcaggaactc caggtagctg acggcgttgg ccggcaggtc gtggcgctca cggaccccgg
     4201 tcaggtcctg ttgccaaccg ggcagctcct cgtagaccgg cgtggcggcg tggaggtcgg
     4261 actggtggta cggcaggtgg tcgtggcgga cgccgtcgac ctcgtacgcg acgcagacct
     4321 tgatggtctc gaacgtgtcg aagatgtcga gcttggtgat cgcgacctcg gacagcgagt
     4381 tgagccgcac cgcgtggcgc agcatcaccg cgtcgaacca gccggggcgg cgacgacgct
     4441 tggtgttgac gccgtactcg tggccgaggt cgacgatgcg gtcaccggtc tcgtcgaaca
     4501 gctcggtcgg catcgggccg gacccgacgc gggtcacata cgccttggcg atgccgatga
     4561 cccggtcgat gtggcgcggg ccgaccccgg cgccgacgca cgccccgccc gcgatcgggt
     4621 tggacgaggt gacgaacggg tacgtgccgt ggtcgaggtc gaggaacgtg gcctgggcgc
     4681 cctcgaacag gacgtgctcg ccgcgctcca acgactcgtg caccagcgac acggtgtcgg
     4741 ccacgtacgg ggcgacccgc ggcgcgac
//
LOCUS       DAOQOD010000216         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7670_length_4738_cov_9.847534, whole genome shotgun sequence.
ACCESSION   DAOQOD010000216 DAOQOD010000000
VERSION     DAOQOD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7670_length_4738_cov_9.847534"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLZ72_14600"
     CDS             <1..361
                     /locus_tag="PLZ72_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37575.1"
                     /translation="AAGVAPGAAETPTELARRVRDSDVFGALDELDRPLGRPGDDGAD
                     RDRPDRPDNPDRAAVDAAVDTLARIETARRYGPTPPDDDDALAAEQAASVLHRAVWSM
                     LGRRDRVRQLVGSTGRS"
     gene            complement(393..776)
                     /locus_tag="PLZ72_14605"
     CDS             complement(393..776)
                     /locus_tag="PLZ72_14605"
                     /inference="COORDINATES: protein motif:HMM:NF022684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HNA37576.1"
                     /translation="MPLSEEEQRILSEIESQLRASDPDLARQVGSTTIYSHALRQLRW
                     GIVGFIAAFALTIFLLTLSVILAFTGFLAMVACAIVVERAARRMGRAGIGEAGHALRA
                     AGLRGYVNGAGRRTRDRFTSKDDDA"
     gene            complement(978..2303)
                     /locus_tag="PLZ72_14610"
     CDS             complement(978..2303)
                     /locus_tag="PLZ72_14610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNA37577.1"
                     /translation="MGGRDILHVDMDAFFASVELLRRPELRGRPVAVGGTGDRGVIAA
                     ASYEARVFGVRSAMPSVRARRMCPDLVLLPGDHAHYSEVSARLMDMFRSVTPLVEPLS
                     LDEAFLDVTGARRSQGPPTEIAAGLRRRVHDQEGLGCAVGVAPNKFLAKLASAAAKPR
                     VGPDGSSPGAGVLVVEPGRELSFLHPLDVGRLWGVGPATLAKLQRFGVATVGDLAAVP
                     RDVLERALGRAAGGHLHDLAHAIDDRPVVPDQEPKSISHEETFPTDRRAIDDLRADVV
                     RMADAVAARLRRHGVRARTVQVKLRYATFDTVTRSHTLDVPTDRADRVRDEAWALLST
                     LPVERGVRLLGVGTTNLTREAPAEQLALDGFEPERTSEGTSEGTWDGAPAPVSEADRE
                     AATAAVDAIRRRFGPSSIGPGRLVHGPGTAAGPPTPRGPWGPDRPPSTP"
     gene            2391..2924
                     /locus_tag="PLZ72_14615"
     CDS             2391..2924
                     /locus_tag="PLZ72_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNA37578.1"
                     /translation="MPTDRSDLRSLLAADRAANRDSGKGRLVTAAFRLTAAARDRGDG
                     GLLGRVATLAYRLGVDWVLGIDLPPETKVGPGLAIFHGTGLVVHPTTVIGSDVMVRHG
                     ITLGAAGDGRTGDEGTGPRIGDRVTIGAGAIVLGPITVGDDAVIGAGAVVVNDVPDGA
                     VVVGNPARVVRIREQLR"
     gene            2921..4105
                     /locus_tag="PLZ72_14620"
     CDS             2921..4105
                     /locus_tag="PLZ72_14620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA37579.1"
                     /translation="MTDRDAVHVLVVAHGPPLRGGIATVALDLVEDPGLNDEFDIEFL
                     NTAQNDEARGRFSLANVGRVSRDAVETFRRSRPGTVVHTHSVHYPAPVVWRQVAVAVA
                     ARLRGAAVINHNHAQPPYMEGPGEWQIGRANAWGFAVLDRLVEATVLIAAAGVPNIRR
                     YMPSSPLPVVHNSIVVDEVVASTAVHEPPVVLFIGELLERKGLVVLLDAVDLLAERGV
                     VAGVDYELRIVGDDRPGLDPLKDEMVAQVQARGFGDALTGPLERAEVYRHLSESDLYV
                     FPTFTEGQPFTVIESLASGVPLVATDIQAIANMVEDPTHGRLVPIRDAVAVADAVQSL
                     LADPDERRRISIACRERARERFDRPVFRARIAALYRAAGNNRGRRARLRAGRARIVAI
                     DR"
     gene            complement(4170..>4738)
                     /locus_tag="PLZ72_14625"
     CDS             complement(4170..>4738)
                     /locus_tag="PLZ72_14625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNA37580.1"
                     /translation="RAALGPPFGQAGVVRAWTVASAVIETGDLPGLPDDLPPGAVLLV
                     ENRRPNGTSDWTTPGGVVDEGEEPLDGLTREVAEETGLRVIRWGGLLYDIEASAPDLG
                     WNLRVEVHRAVAVAGELSIGSDPDGIVVGSAWAASAACVELLGDSHPWVREPLLDWLE
                     NRWTDPRTYSYVIRGTRPDEFRVERPDR"
BASE COUNT          616 a         1763 c         1734 g          625 t
ORIGIN      
        1 ggctgcgggt gtcgcacccg gcgcggccga gaccccgacg gagctggccc gccgcgttcg
       61 ggactcggac gtgttcggcg cgctcgacga gctcgaccgc ccgctcggcc gaccgggcga
      121 cgacggggcg gaccgggacc gtccggatcg tccggacaat ccggaccggg ccgcggtcga
      181 cgccgcggtg gacacgctgg cgcgcatcga gaccgctcgt cgctacggac cgaccccgcc
      241 cgacgacgac gatgcgctgg ccgccgagca ggccgcatct gtcctgcacc gggcggtgtg
      301 gtcgatgctc ggccgacgcg accgggtgcg tcagctggtc ggatcgaccg gtcggtcctg
      361 agagagcccg gccgtcgcac cgctcagtcg ggtcaggcgt cgtcgtcctt ggacgtgaac
      421 cggtcccggg tccggcgacc cgcaccgttc acgtatcccc gcaggcccgc ggcgcgcagc
      481 gcgtgtccgg cctcgccgat gccggcccgg cccatccgcc gggcggcccg ctcgacgacg
      541 atggcgcacg ccaccatggc caggaagccg gtgaaggcca ggatcacgct gagggtgagg
      601 aggaagatcg tcaacgcgaa ggcggcgatg aagccgacga tgccccagcg gagttgacgg
      661 agcgcgtggg agtagatcgt cgtcgagccg acctggcggg ccaggtcggg gtcgctggcg
      721 cgcagctgac tctcgatctc gctgaggatt cgctgctctt cctcggagag cggcatgggc
      781 ggtgccccct cgctgcgtcg gactcgtggg tcggacccgg ttgcgtcgga ccgggctgac
      841 ggaaccgatc cggctccgtc cgaatgttcc cacacgctac ccggcgacgc cacggcatcg
      901 gccgtcaggt gccggctttc ccgcgcgacc tcggcggaca cgtcgtcgag gtcggccgtc
      961 gcgggtcggt tccgcgctca cggcgtcgac ggtgggcggt cgggtcccca cggtccgcgg
     1021 ggtgtcggag ggccggcggc ggtgcccggg ccatgcacca ggcgtcccgg cccgatgctc
     1081 gacggtccga accgccggcg tatggcgtcg acggccgcgg tggccgcctc gcggtccgcc
     1141 tcgctgaccg gtgccggagc tccgtcccag gtgccctccg aggtgccctc cgaggtgcgc
     1201 tccggctcga acccgtcgag cgcgagctgc tcggcgggcg cctctcgggt gaggttcgtg
     1261 gtgcccacgc cgagcagtcg gaccccccgc tcgaccggca gggtcgacag cagggcccat
     1321 gcctcgtcgc ggacgcggtc ggcgcgatcg gtcgggacgt cgagggtgtg cgagcgggtc
     1381 acggtgtcga aggtggcgta gcgcagcttc acctgcaccg tccgggcccg caccccgtgc
     1441 cggcggaggc gtgcggccac cgcgtcggcc atccgcacga cgtcggcccg caggtcgtcg
     1501 atcgcccgcc ggtcggtcgg gaacgtctcc tcgtggctga tcgacttggg ctcctggtcc
     1561 gggacgaccg gccgatcgtc gatcgcgtgg gccagatcgt gcaggtggcc accggccgcc
     1621 cgacccaacg cccgctcgag gacgtccctg ggcacagccg ccaggtcccc caccgtcgcc
     1681 accccgaagc gctggagctt ggccagcgtg gcgggcccca cgccccacag ccgacctacg
     1741 tccagggggt gcaggaacga cagctcacgg cccggctcga ccaccaggac gccggcgccg
     1801 ggcgaggacc cgtccgggcc gacccggggc ttggccgccg cggacgcgag cttggccagg
     1861 aacttgttgg gtgccacccc caccgcacag cccagaccct cctggtcatg gacgcgccgg
     1921 cggagcccgg ccgcgatctc cgtcggcggt ccctgcgacc gacgggcccc ggtcacgtcg
     1981 aggaacgcct cgtcgagcga cagcggctcg accagcgggg tgacggagcg gaacatgtcc
     2041 atcagacggg cggagacctc ggagtagtgc gcgtggtctc cgggcagcag caccagatcc
     2101 gggcacatcc gtcgggcgcg caccgacggc atggccgacc gcacgccgaa cacgcgggcc
     2161 tcgtaggacg ccgccgcgat caccccccgg tcgccggttc cgccgaccgc caccggccgt
     2221 cccctcagct cgggtcgccg gagcagttcg accgaggcga agaaggcgtc catgtcgaca
     2281 tgcaggatgt cccgaccgcc caccgggcca gtctcgcacc cgcgtcccga acccggggcg
     2341 gggccgccca cgcgctcatc ggcgtcgcac cccgctaccg tcagcatccc atgcctaccg
     2401 accgttccga cctgaggagc ctcctcgccg ccgaccgcgc cgccaaccgc gactcgggca
     2461 agggccggct cgtgaccgct gcgttccgcc tcaccgccgc cgcccgcgac cgtggcgacg
     2521 gagggttgct cgggcgggtg gccacgctgg cctaccggct gggggtcgac tgggtgctcg
     2581 ggatcgatct gccccccgag acgaaggtgg ggccggggct cgcgatcttc cacggcaccg
     2641 gactggtcgt gcatccgacg acggtgatcg gctccgacgt catggtgcgc cacggcatca
     2701 cgttgggcgc ggccggtgac ggccggaccg gcgacgaagg gaccggaccc cgcatcggcg
     2761 accgggtgac catcggcgcg ggtgcgatcg tcctcggccc gatcaccgtc ggcgacgacg
     2821 ccgtcatcgg cgccggcgcg gtggtcgtga acgacgtccc cgacggagcg gtcgtggtcg
     2881 gcaacccggc gcgggtcgtt cgcatccggg agcagctccg atgaccgacc gcgacgccgt
     2941 gcacgtcctc gtcgtcgccc acgggccacc gctgcggggt gggatcgcca ccgtcgctct
     3001 cgacctggtc gaggacccgg gcctgaacga cgagttcgac atcgagttcc tcaacaccgc
     3061 ccagaacgac gaggcccggg gtcggttctc gctcgcgaac gtcgggcgcg tgagccggga
     3121 cgcggtcgag acgttccgcc gatcccgtcc cggcaccgtc gtgcacaccc actcggtgca
     3181 ctacccggct ccggtcgtgt ggcgacaggt cgcggtcgcg gtcgcggccc gcctgcgggg
     3241 agccgcggtg atcaaccaca accacgccca gccgccatac atggaggggc cgggcgagtg
     3301 gcagatcggt cgggcgaacg cgtggggctt cgccgtgctc gaccgcctgg tcgaggcgac
     3361 cgtgctgatc gcggcggccg gggtgccgaa catccggcgc tacatgccga gctcgccgct
     3421 gccggtggtc cacaactcga tcgtcgtcga cgaggtcgtg gcctcgaccg cggtgcacga
     3481 gccgccggtg gtcctgttca tcggggagct gctcgagcgc aagggcctgg tcgtcctgct
     3541 cgacgctgtc gacctgctgg cggaacgggg cgtggtggcc ggcgtcgact acgagctgcg
     3601 gatcgtcggc gacgaccgcc cgggtctcga cccgctcaag gacgagatgg tcgcccaggt
     3661 ccaggcccgc gggttcggcg acgcgctgac cggcccgctg gagcgcgccg aggtgtaccg
     3721 ccacctctcg gagtccgacc tgtacgtctt ccccaccttc accgagggcc agccgttcac
     3781 ggtgatcgag tcgctcgcat cgggggtccc gttggtggcc accgacatcc aggcgatcgc
     3841 gaacatggtc gaggacccga cccacgggcg gctggtcccc atccgcgacg cggtcgcggt
     3901 cgcagacgcc gtccagtccc tgctggcgga ccccgacgag cgtcggcgga tctcgatcgc
     3961 gtgtcgagag cgggcgcggg agcgattcga ccgcccggtc ttccgggccc ggatcgcggc
     4021 gttgtaccgc gccgcgggca acaaccgggg tcgccgcgcc cgcctccggg ccggccgggc
     4081 ccgcatcgtc gccatcgacc gctgacctgc cctgctggtc cgtgttaccg gcccgcaccg
     4141 ggccgggcgc gagtcggacc ggcgcgggcc tagcggtccg ggcgctccac ccggaactcg
     4201 tccggtcggg tgccgcggat cacgtaggag taggtgcggg gatcagtcca gcggttctcg
     4261 agccagtcga gcaagggctc tcgcacccac gggtgcgagt cgccgaggag ctcgacgcac
     4321 gcggcggagg ccgcccaggc ggacccgacg acgatgccgt cggggtccga cccgatcgag
     4381 agctccccgg ccaccgcgac ggcccggtgg acctcgacgc ggaggttcca gccgagatcg
     4441 ggagccgatg cctcgatgtc gtagaggagg cctccccagc ggatgaccct cagcccggtc
     4501 tcctcggcga cctcacgggt caacccgtcc aggggctcct cgccctcgtc gaccacaccg
     4561 cccggggtgg tccagtccga tgtcccgttg ggtcgtcggt tctcgaccag gaggacggcg
     4621 ccggggggca ggtcgtcggg gaggccgggg aggtcccccg tctcgatcac cgcgctggcg
     4681 acggtccagg ctctcaccac accagcctgc ccgaacggcg ggcccagcgc cgccctgc
//
LOCUS       DAOQOD010000217         4713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7735_length_4713_cov_13.213826, whole genome shotgun sequence.
ACCESSION   DAOQOD010000217 DAOQOD010000000
VERSION     DAOQOD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_4713_cov_13.213826"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLZ72_14630"
     CDS             <1..355
                     /locus_tag="PLZ72_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37581.1"
                     /translation="LGAWLWAAEAAYGAADAARREGDPRMAAHWSRRATEWRAACETA
                     ATPGLVADLAPVPLTRREREVAILAADGLAAREIGERLYVSRRTVESHLARVYTKLGI
                     GSRAELASLLQDTPR"
     gene            complement(390..1277)
                     /locus_tag="PLZ72_14635"
     CDS             complement(390..1277)
                     /locus_tag="PLZ72_14635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNA37582.1"
                     /translation="MSRTPDRSGDPSPTDRDDPLADALDGLEPAAVAYLDLLQRCLTR
                     EVFLDQEWWDADLRDWPGGADQVLPLLRERDWRVVRRGDPDARAEGRDWPPTAETMIG
                     SARLDNVVRCCVRALTDGVPGDLIETGVWRGGTTILMRGVLRALGDPDRRVWVADSFE
                     GLPVPDVRYPADDGLDWSHVEVLKVGADAVRANFERYGLLDDRVRFLEGWFADTLPDA
                     PIEQIAVLRLDGDLYGSTMDALSALEPKVAPGGFVVIDDYGGWEPCRAAVQDYRRDHG
                     ISAPIRTVDWTGVWWRKPG"
     gene            1301..1735
                     /gene="arfB"
                     /locus_tag="PLZ72_14640"
     CDS             1301..1735
                     /gene="arfB"
                     /locus_tag="PLZ72_14640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNA37583.1"
                     /translation="MAGTDDPDPRGVVVRPGLHIPAAWIDESFSTSGGPGGQHANRSR
                     TRVELRLDLRGCPTFSEHQRSLLVERFGPEIRVVVDDERSQLRNRDIARERLAARIAN
                     ALVPQRTRTATRATRASQRRRVEAKKRRGDVKRQRRRPDHDD"
     gene            complement(1818..2828)
                     /locus_tag="PLZ72_14645"
     CDS             complement(1818..2828)
                     /locus_tag="PLZ72_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNA37584.1"
                     /translation="MPETIQRIVLRRRPEGLVTWDDVELVTAPVPVPGPGEALLRNEV
                     LAIDASVRSWLSPARGYLPPVEIGEAVRCSSAGRIVATDCDAYGLDDVVTSLAAWEEY
                     SIVRDDIFTTRLSGPDPDYDTTGYLSLYGSTGCTAYIGMIEVGEVKAGDVVVVSAAAG
                     ATGSIAAQIAKLHGARVIGIAGSDDKCAWLLDEVGLDGAINHRTEDVSARIKELGPRV
                     DLFFDNVGGAVLDAVLSRLALHARVVLCGAISTYNESSKAPGPANYLQLIQQRATMTG
                     FLSLDDVPRFPEIGEVLRAWEREGRLRYHADPHHGLGSSVDALNAMFTGANTGKVVIT
                     LP"
     gene            complement(2843..3628)
                     /locus_tag="PLZ72_14650"
     CDS             complement(2843..3628)
                     /locus_tag="PLZ72_14650"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNA37585.1"
                     /translation="MSAEVTDSTGPGRHGSGEVEWGEVEGVTIRFPLVVDEMHSATLT
                     FTVPFDAARALVPGDAFEVLEAGPGSAMLVIALCDYLRNPWGDYNEVNVGILVHPVGR
                     PEAAGAFVWRMPVDQEFTCAAGRQVMGVPKTVEDITFTYDGEPGDPDATVTMRLVMAD
                     EPAGSATLQVRFPRPSSEGVDASVEPAMTYAYLDGIPMELGLDMELPPLLLDPATVEV
                     TLGQGALADELRTLGLPERPADLAMWGESLAATFQVPRPVSGS"
     gene            3757..4449
                     /locus_tag="PLZ72_14655"
     CDS             3757..4449
                     /locus_tag="PLZ72_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37586.1"
                     /translation="MDHVRAVIHPFRRLRRRGERAATLVEYALLVAVFVVPASAGLSF
                     MRDSAKSKMGHVATQVAKSPTTTTTEAGGGGGGGGSSTSTTAAPTSTTTTTTPTTTTT
                     STPPTSTTVAKVAKTTAPTLTTSKSGSGSSKRWKVDGSVKITDNTGAAVNGATVTFKV
                     EEIDKYGSVTATTTVTGTTNSSGNATLTVSNLTSSDMTVQVTVTNVTGLPAGTTWNGS
                     STNSQANRPSPW"
     gene            4475..>4713
                     /locus_tag="PLZ72_14660"
     CDS             4475..>4713
                     /locus_tag="PLZ72_14660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNA37587.1"
                     /translation="MADSTAISLLVIAVAAVAAPILSELLSRWHVPGVLFELLLGIVI
                     GPAVLGWAQVDTFVKGLSTLGLGFLFFVAGYEIDTA"
BASE COUNT          694 a         1754 c         1636 g          629 t
ORIGIN      
        1 cctcggcgcc tggctgtggg ccgccgaggc cgcctatggg gcggccgacg ccgcccgccg
       61 cgagggcgac ccccgcatgg ctgcgcactg gtcgcggcgg gcgacggagt ggcgagccgc
      121 gtgcgagacc gcggccaccc ccggactggt ggccgacctg gctccggtgc ctctgacccg
      181 tcgcgagcgc gaggtggcga tcctggccgc cgacggcctg gccgctcgtg agatcggcga
      241 gcgcctctac gtctcgcgcc ggaccgtgga gtcacatctg gcgcgggtct acaccaagct
      301 cggcatcggc tcccgggccg aactggcgtc cctcctgcag gacacgcccc gctgaccggc
      361 gtgccctgcc gggccacggc tcggccggat caacccggct tgcgccacca cacaccggtc
      421 cagtcgacgg tgcggatcgg cgcgctgatg ccgtggtccc ggcggtagtc ctgcaccgcg
      481 gcgcgacacg gctcccatcc gccgtagtcg tcgatcacca cgaacccgcc cggcgccacc
      541 ttcggctcga gggcgctgag cgcgtccatg gtcgagccgt acaggtcgcc gtcgaggcgc
      601 agcaccgcga tctgctcgat cggggcgtcg gggagggtgt cggcgaacca gccctcgagg
      661 aaccggaccc ggtcgtcgag caggccgtag cgctcgaagt tggctcgcac ggcgtccgct
      721 ccgaccttga ggacctcgac gtgggaccag tcgagtccgt cgtcggccgg gtagcggacg
      781 tcggggaccg ggagcccctc gaaggagtcc gcgacccaca cgcggcggtc ggggtccccg
      841 agggcgcgca gcacgccgcg catcaagatg gtggtacccc ctcgccagac cccggtctcg
      901 atcaggtcgc cggggacccc gtcggtgagg gcccggacgc agcagcgcac gacgttgtcc
      961 agccgggcgg agccgatcat ggtctccgcg gtcggcggcc agtcccgacc ctcggcgcga
     1021 gcatcggggt ctccgcgtcg gaccacccgc cagtcgcgct cacgcagcag cggcaggacc
     1081 tggtcggccc ctccgggcca gtcgcgcagg tccgcgtccc accactcctg gtccaggaac
     1141 acctcccggg tgaggcaccg ctggagcagg tcgaggtacg cgaccgccgc cggctccagg
     1201 ccgtcgagcg catcggccag cgggtcgtcc cgatcggtgg gcgatgggtc tccggaacgg
     1261 tccggggtgc gcgacatggc ggcgacggta ccctccgacg atggccggca ccgacgaccc
     1321 cgacccccgc ggtgtcgtcg tccgtcccgg cctccacatc ccggcggcgt ggatcgacga
     1381 gtcgttctcc acctcgggcg gtcccggcgg ccagcacgcc aaccggtccc gcacccgggt
     1441 cgagctgcgg ctcgacctcc ggggctgccc caccttcagc gagcaccagc ggtcgctcct
     1501 ggtggagcgg ttcggaccgg agatccgggt ggtcgtcgac gacgagcgct cgcagctgcg
     1561 caaccgcgac atcgcccggg aacggctggc ggcgcggatc gccaacgcgc tggtcccgca
     1621 gcggacccgg accgccaccc gggcgacgcg ggcgtcgcag cgacgacggg tggaggccaa
     1681 gaagcgtcgg ggcgacgtca agcgccagcg ccgccgcccc gaccacgacg actaaccctc
     1741 tccctcaccc ccgctttgtc ccgcacaggc gcggtgatca ccgcgcctgt gcgggacaaa
     1801 gcggggagga gaaggggtca gggaagggtg atgacgacct tgccggtgtt ggcgccggtg
     1861 aacatcgcgt tgagggcgtc gaccgaggac ccgagcccgt ggtgcggatc cgcgtggtac
     1921 cgcaggcgcc cctcgcgctc ccaggcccgc agcacctcgc cgatctcggg gaaccggggg
     1981 acgtcgtcga gcgacaggaa cccggtcatg gtcgcccgct gctggatcag ctgcaggtag
     2041 ttggccggac cgggggcctt ggaggactcg ttgtaggtcg agatcgcacc gcacagcacc
     2101 acccgggcgt gcagcgccag acgactgagc acggcgtcga gcacagcgcc tcccacgttg
     2161 tcgaagaaca ggtcgacgcg gggtcccagc tccttgatcc gcgccgagac gtcctcggtt
     2221 cggtggttga tcgccccgtc caggccgacc tcgtcgagca gccaggcgca cttgtcgtcg
     2281 ctgccggcga tcccgatgac ccgggcaccg tgcagcttcg cgatctgcgc cgcgatcgac
     2341 ccggtcgccc ccgcggcggc cgacacgacg accacgtcac cggccttcac ctcgccgact
     2401 tcgatcatgc cgatgtaggc ggtgcatccg gtcgacccgt acagcgacag gtagccggtg
     2461 gtgtcgtagt cggggtcggg gcccgacagc cgggtggtga agatgtcgtc gcggacgatc
     2521 gagtactcct cccacgccgc caacgaggtg acgacgtcgt cgagcccgta ggcgtcgcag
     2581 tcggtggcga cgatgcgccc ggccgacgag cagcgcaccg cctcgccgat ctcgaccggc
     2641 ggcaggtagc cgcgggccgg gctgagccag gagcgcaccg acgcgtcgat ggccaggacc
     2701 tcgttgcgca ggagcgcctc cccggggccg ggcacgggaa ccggcgcggt caccagctcg
     2761 acgtcgtccc aggtgacgag cccctcgggc cgccgacgca gcacgatgcg ctgtatcgtc
     2821 tccggcacgg accccgccgc gctcacgagc cggagaccgg tcgcggcacc tggaaggtgg
     2881 cggccaggct ctcgccccac atcgcgaggt ccgccggacg ctcgggcaga ccgagggtgc
     2941 gcagctcatc ggccagggcg ccctggccca gggtcacctc gaccgtggcg gggtcgagca
     3001 gcaacggcgg cagctccatg tccagaccga gctccatcgg gatgccgtcg aggtaggcgt
     3061 aggtcatggc cggctccacc gaggcgtcca ccccctccga cgacggccgc gggaaccgga
     3121 cctgcaacgt cgcggagccg gccggttcgt cggccatcac cagtcgcatg gtcactgtcg
     3181 cgtcggggtc gcccggctcg ccgtcgtagg tgaaggtgat gtcctcgacg gtcttgggga
     3241 cgcccatgac ctgccgaccg gcggcgcagg tgaactcctg gtcgaccggc atccgccaca
     3301 cgaacgcccc tgccgcctcg ggccgaccga ccgggtggac caggatcccg acgttgacct
     3361 cgttgtagtc accccacgga ttgcgcaggt agtcgcacag ggcgatgacg agcatggcgg
     3421 agcccgggcc ggcctcgagc acctcgaagg catcgccggg gaccagggcc cgcgccgcgt
     3481 cgaacgggac ggtgaaggtc agcgtcgccg agtgcatctc gtccaccacc aacgggaagc
     3541 ggatcgtcac cccctcgacc tcaccccact cgacctcacc cgatccgtgg cggcccgggc
     3601 ctgtgctgtc cgtcacctct gcgctcatcc gcgggactgt acgccgccca ccggtgcgac
     3661 tgggccgaac cacccaacag gcggggccga acgacccatg cacgaacggc tcttcgcccc
     3721 atgcagatca cgtgcgcgcg tcgtcaggat ggatccatgg atcacgtccg cgctgtcatc
     3781 catcccttcc ggcgtctccg tcgccgcggc gaacgggccg ccaccctggt cgagtacgcc
     3841 ctgctcgtcg cggtgttcgt ggtccccgcg tcggccggcc tgtcgttcat gcgggactcg
     3901 gccaagtcga agatgggcca cgtggccacc caggtcgcca agtcgccgac caccaccacg
     3961 accgaggccg gcggcggagg cggaggcggt ggttcgtcca cctccacgac cgcggctccg
     4021 acgtcgacga cgaccaccac caccccgacc acgaccacca cctcgacccc gccgacgagc
     4081 acgacggtcg ccaaggtggc caagaccacc gcccccaccc tgaccacctc caagtcgggt
     4141 tcgggcagct cgaagcgctg gaaggtcgac gggtcggtca agatcaccga caacaccggc
     4201 gccgcggtga acggggcgac cgtgacgttc aaggtcgagg agatcgacaa gtacggcagc
     4261 gtcaccgcca ccacgacggt caccgggacc acgaactcgt cgggcaacgc cacgctgacg
     4321 gtgtcgaacc tgacctcgtc ggacatgacg gtgcaggtca cggtgaccaa cgtgacgggt
     4381 ctgccggccg gcacgacctg gaacggctcg tcgaccaatt cccaggccaa ccgacccagc
     4441 ccctggtgat cgcggcggga gctagcctcc cgctgtggcc gactcgaccg cgatcagcct
     4501 tctggtgatc gcggtcgctg cggtggcggc cccgatcctg tcggagctgc tgtcccgctg
     4561 gcacgtcccc ggggtcctgt tcgagctgct gttggggatc gtgatcggcc ccgcggtcct
     4621 cggttgggcc caggtcgaca ccttcgtcaa gggcctgtcc acgctcggcc tcgggttcct
     4681 gttcttcgtc gccggctacg agatcgacac cgc
//
LOCUS       DAOQOD010000218         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_7784_length_4697_cov_11.892503, whole genome shotgun sequence.
ACCESSION   DAOQOD010000218 DAOQOD010000000
VERSION     DAOQOD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7784_length_4697_cov_11.892503"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1194)
                     /locus_tag="PLZ72_14665"
     CDS             complement(232..1194)
                     /locus_tag="PLZ72_14665"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNA37588.1"
                     /translation="MSDRSTGNPEGIDGMVLVPGGETLVGSDRFYPEERPVHTVTVPS
                     LYVDTHPVTNDRFAEFVADTGWVTVAERPLDPADYPGADPDLLHPGGVVFRPPPGPVP
                     LDDPSRWWAYVPGASWRHPRGPGSDLSGLGDHPVVQVSFQDAAAFAEWRGARLATEAE
                     WERAARGGLVGAEYCWGDEPYPDDRQLANTWQGRFPWEDLALDGWSGTSPVGSFPANG
                     YGLFDMAGNVWEWTTDWWSDERGSSGGACCSGGVVRDAPAMDEAEREGASAAPGERHG
                     RKVIKGGSHLCAPSYCHRFRPAARQPEALDTSTCHIGFRCVLDA"
     gene            1360..2571
                     /locus_tag="PLZ72_14670"
     CDS             1360..2571
                     /locus_tag="PLZ72_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37589.1"
                     /translation="MTDGNDRYTVISADTHAGGSHAMYREYLEEKYLDDFDAWRDKYK
                     NPFSDLGDQRRLRNWDTEMRTRQQDEDGVAGEVIFPNTVPPFFPSFVLFARPPKADEY
                     EHRLAGIRAHNRWLVDFCAEQPERRKGIGQIFINDIDDAIDDVRWIKDHGLTGGVLLP
                     NVPPDVDWVKQLNHPDYDRLWAVCQELDVPINVHGGTGTPAYEPLPSSALMMIAEVPY
                     FSRRPLLFMLLSGAFERFPGLKFVLTEQGCAWLPELVQYLDGHLESIRANGAIGELRF
                     KPEHILPKNASDYVRQNCWFGLSFPLKADLDAAVATVGIDHVMWGSDYPHDEATSPYS
                     NIALRQVFHDWDPADVRKVLTENPAELYGFDVDALAPVAERIGPTVAEIAQPVTELPA
                     EANMALLRNAS"
     regulatory      2928..3019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3126..4625
                     /locus_tag="PLZ72_14675"
     CDS             3126..4625
                     /locus_tag="PLZ72_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNA37590.1"
                     /translation="MTNATTRRRRPGAAAAMLIGTGLVLGLLGTVRSGAATPTSPRPT
                     AEATAALDWLAGQITSHGGALPGPVAGTPDWGLTADAVLAFASAGRSTDPAAVAATDQ
                     LVAHAADFTTYTSVGVTVRVAGPTAKVLLVALSTGRSATVGGVDLDAALRGLMRSSGP
                     QTGRFADGAPDPTWDGSNGFGQSLAMLALARTDRGVPAPAVEFLLAQQCPAGGFRLNY
                     GTAAGCGTDAEADSDATAVSLQALLAVERSPDVLAALERGTAWLRSRQGAEGSFGGTG
                     PTAAPNANSTGMIAQFLRAAGDTTAADRAAGWIAACCQLSAQNATGTPASGHIGAIGY
                     DPAARTSALADGIGAGVSDQWRRTTTQAVLAFGLAPYGPPVEPLPPTGPTTSAPGSSS
                     TIPTTSPSSTTPSSTTPSDTTPSNTAPSSTVAGVELGPATQATAPVSVQSEQLDAEAA
                     AAGTSPTSGSVQGSSLARTGTDSRWLLTAALVLLGSGAALATAERREGG"
BASE COUNT          697 a         1829 c         1582 g          589 t
ORIGIN      
        1 catccaccgc accggatggc agatgacccc caacccccag accgacggag cctcaggaga
       61 cggcgctgac ggcgatcaga ccagcttctt caccaccacc acccccgacg gcgcccgact
      121 ccccacccac cacgaccggc cacccagacc cgcacccgcc tgagcgcggg accgacaccg
      181 gaccaaccca cgccccccga gcgaggtcct tcccgacctg atggtcggcg ttcaggcgtc
      241 gagcacgcag cggaacccga tgtgacacgt cgaggtgtcg agcgcctcgg gctggcgcgc
      301 cgcggggcgg aaccggtggc agtagctcgg cgcgcacagg tgcgatccgc ccttgatcac
      361 ctttcggccg tggcgctccc cgggtgctgc cgaggcaccc tcgcgctcgg cttcgtccat
      421 tgccggcgcg tcccggacca ctcccccgct gcagcacgcc ccgccggatg atccgcgctc
      481 gtcgctccac cagtcggtgg tccactccca cacgttgccg gccatgtcga acagcccgta
      541 gccgttggcc gggaacgacc ccaccggcga cgtgcccgac cagccgtcga gggccaggtc
      601 ctcccagggg aaccgcccct gccaggtgtt ggcgagctgg cggtcgtccg ggtagggctc
      661 gtcaccccag cagtactcgg cgccgaccag gccgccgcgg gccgcccgct cccactccgc
      721 ctcggtggcc agacgggctc cgcgccactc ggcgaacgcg gcggcgtcct ggaagctcac
      781 ctgcaccacc gggtggtcgc cgagaccgga gaggtccgag cccggcccgc gggggtgacg
      841 ccacgacgcc cccggcacgt acgcccacca ccgcgacgga tcgtcgaggg gcaccggacc
      901 cgggggcggg cggaacacga cgccgcccgg gtgcagcagg tcggggtccg cgcccgggta
      961 gtccgccggg tcgagaggcc gctcggcgac ggtgacccaa ccggtgtcgg ccacgaactc
     1021 ggcgaagcgg tcgttggtga ccgggtgggt gtcgacgtac agcgacggca cggtcacggt
     1081 gtggaccggt cgctcctcgg gatagaagcg gtcggatccg accagggtct cgccgccggg
     1141 gaccagcacc atcccgtcga tgccctctgg gttgcccgtg gaccggtcgc tcacgtccgg
     1201 aggctacgcg tcgcccacac cggggtcgga cctcgaaact gacactagaa ttcggtttca
     1261 gtttctgcca tgctgggggc aggcccccga gagcgggccg cggccgacgg ggaccgcggt
     1321 ccacgggccc gacccaaccc atcgcccgaa gggacgacca tgaccgacgg caacgaccgc
     1381 tacaccgtga tctccgccga cacccacgcc ggcggcagcc acgcgatgta ccgcgagtac
     1441 ctcgaggaga agtacctcga cgacttcgac gcctggcgcg acaagtacaa gaacccgttc
     1501 agcgacctcg gcgaccagcg ccgcctgcgc aactgggaca ccgagatgcg cacccgccag
     1561 caggacgagg acggcgtcgc gggcgaggtc atctttccca acaccgtgcc gccgttcttc
     1621 ccgagcttcg tgctgttcgc ccgcccgccc aaggccgacg agtacgagca ccgcctggcc
     1681 ggcatccgcg cccacaaccg atggctggtc gacttctgcg ccgagcagcc cgagcgacgc
     1741 aagggcatcg gccagatctt catcaacgac atcgacgacg ccatcgatga cgtgcgctgg
     1801 atcaaggacc acggcctcac cggcggggtc ctgctgccca acgtgccgcc cgacgtcgac
     1861 tgggtcaagc agctcaacca ccccgactac gaccgactgt gggcggtgtg ccaggagctg
     1921 gacgtgccga tcaacgtgca cggcggaacc ggcaccccgg cgtacgaacc gctgccgtcc
     1981 tcggcgctga tgatgatcgc cgaggtcccc tacttcagcc ggcgcccgct gctgttcatg
     2041 ctgctgtcgg gcgcgttcga gcggttcccc ggcctcaagt tcgtgctcac cgagcagggc
     2101 tgcgcctggc tgcccgagct ggtccagtac ctcgacggcc acctggagtc gatccgggcc
     2161 aacggggcga tcggcgagct gcggttcaag ccggagcaca tcctcccgaa gaacgcgtcg
     2221 gactacgtgc gccagaactg ctggttcggg ctcagcttcc cgctgaaggc cgacctcgac
     2281 gccgcggtcg ccacggtcgg catcgaccac gtcatgtggg gcagcgacta cccccacgac
     2341 gaggccacct cgccgtactc caacatcgcc ctgcgccagg tgttccacga ctgggatccc
     2401 gcggacgtgc gcaaggtgct gaccgagaac cccgccgagc tctacgggtt cgacgtcgac
     2461 gcgttggcgc cggtcgccga gcgcatcggc ccgacggtgg ccgagatcgc ccagcccgtc
     2521 accgagctgc cggccgaggc caacatggcg ctgctgcgca acgcctcctg accgcgtcgg
     2581 tcgacgcgtc gtgatgagcg atcccgctca tcacgacgcg accgaccgtg atacgcatgg
     2641 cgtcatgcgg acgacggtgg acctcgacgc agacgccagc gctgcggtgg agcggctccg
     2701 acgcgaccag ggactcggcg tgagcacgga gtcccgatcg tctcggcgga cccggacttc
     2761 gcccggttca ccgagctgac ctggatcgac cctctgtcct gaacggggca atgtcctgaa
     2821 ccgggcactg tcctgaacag ggccgcggcc ggtcgacagc gacgggcgct tgcgggtcgg
     2881 agcggccggt gctcagatgg gccgaccatc agccaacccg ccgcgagaga ggaaagccgg
     2941 tcgaagtccg gcactgtccc gcagccgtgg gtcgacgttc ccgccggaga cggcgggcgc
     3001 tcgacgagtc ggatcactcc ccccggcgga ccagcttcga ggaatccgtc gaggactgcg
     3061 gatgccggag cccgggtcga tccgcgcgtc gcggacctcc agggccatcg gccgggagga
     3121 cgaccatgac caacgccacg acccgccggc gacgaccggg cgccgcggcc gccatgctga
     3181 tcgggaccgg cctcgtgctg ggtctcctcg gcacggtccg cagcggcgca gcgacgccga
     3241 cgtcgccccg ccccaccgcc gaggccacag cggccctcga ctggctcgcc ggccagatca
     3301 cctcccacgg cggggccctt cccggcccgg tggccggcac tcccgactgg ggcctgaccg
     3361 cggacgccgt gctcgccttc gcctccgccg gccgctcgac cgacccggcc gcggtcgcgg
     3421 ccaccgatca gctcgtggcc cacgcagccg acttcaccac ctacaccagc gtcggcgtca
     3481 ccgtccgcgt cgccggcccg accgccaagg tcctgctcgt cgcgctgtcg accggccggt
     3541 ccgccaccgt cggcggagtc gacctcgacg ccgccctgcg gggactcatg cgcagctccg
     3601 ggccgcagac cggccggttc gccgacggcg cccccgaccc gacatgggac ggctccaacg
     3661 gcttcggcca gtcgttggcc atgctcgccc tggcccgcac cgaccgcggc gtccccgcgc
     3721 ccgcggtcga gttcctgctc gcccagcagt gccccgcggg cgggttccgc ctcaactacg
     3781 ggaccgccgc tggatgcggc accgacgccg aagccgactc cgacgccacc gccgtgtcgt
     3841 tgcaggccct gctggccgtc gaacgcagcc ccgacgtgct cgccgcgctc gagcgtggca
     3901 cggcgtggct caggtcccga cagggcgcgg aggggtcctt cggcgggacc ggaccgaccg
     3961 cggcgcccaa cgcgaactcg acggggatga tcgcccagtt cctgcgtgcc gcgggtgaca
     4021 cgaccgccgc ggaccgggcc gcgggttgga tcgcggcctg ctgccaactc agcgcccaga
     4081 acgcgacggg cacgcccgcc tcgggtcaca tcggagcgat cggctacgac ccggcggcgc
     4141 ggacctcagc cctggccgac gggatcggtg ccggcgtctc ggaccagtgg cggcggacga
     4201 cgacccaggc ggtgttggcg ttcgggctgg cgccctacgg gccgcccgtc gaacccctac
     4261 cgccgaccgg ccccaccacc tccgctccgg gatcgagctc cacgattccg accacgtcgc
     4321 cgtcgagcac gacaccctcc agcacgacgc cgtccgacac gacaccgtcc aacaccgcgc
     4381 cgtccagcac cgtcgcgggc gtggagctgg gaccggcgac ccaggcgacg gcccctgtgt
     4441 cggtccagag cgaacagctc gacgccgagg cggccgccgc gggcacctcc cccacctcgg
     4501 ggtcggtcca gggcagttcc ctggcccgca ccgggaccga cagccgctgg ttgctgaccg
     4561 ccgcgctggt gctgctcgga tcaggagccg cgttggcgac cgcggagcgg cgggagggcg
     4621 ggtgacaccc ggctcccggg cccgcgtcgt cgctctggcg gtcgctttct gcggcgccgc
     4681 ggtcgcgttg acgagcg
//
LOCUS       DAOQOD010000219         4617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8047_length_4617_cov_8.250548, whole genome shotgun sequence.
ACCESSION   DAOQOD010000219 DAOQOD010000000
VERSION     DAOQOD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8047_length_4617_cov_8.250548"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..441
                     /locus_tag="PLZ72_14680"
     CDS             256..441
                     /locus_tag="PLZ72_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37591.1"
                     /translation="MSRPEEPQETGSIVCVQGTFFQRTGDGPGAVWQDMTWDGMFLLT
                     WNQIAPDAPGVCVEIVR"
     gene            677..1429
                     /locus_tag="PLZ72_14685"
     CDS             677..1429
                     /locus_tag="PLZ72_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37592.1"
                     /translation="MTFTPIGLQASDIAALIATDSAQRPRSRQAAPGPSSLGHPCPRH
                     LAHLRAGTPKTTGGGDPLPSWVGTEAHAGMARILDGLPDWEAEIPVELPGYGIRGTCD
                     AYHRPSRLVLDWKFVGAAAIGDYKANGPGPQYRTQVHLYGLALALTGRDVDRVGIAFI
                     PRAGLSSGIHLWAEDLDEQVCDDALRRWDAIVQVADTLGPAAAPTSGDARCDWCPYYD
                     PGGTDPATSCAGHQPPTVTGTVTEPTNGKARP"
     gene            1426..1872
                     /locus_tag="PLZ72_14690"
     CDS             1426..1872
                     /locus_tag="PLZ72_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37593.1"
                     /translation="MSTFTSPAAASAGITYADHLGHLLIIEVLRYEPTVATSLGDKDA
                     IGATVHDVDLGTTAEDALLFPKVLVASLKNRVGQTVLATLTQGVAKPGQSAPWTLTDA
                     TGDPAAVKRATDFLDAYKSGGLAAPEPAVDAATAAALKLLKDQGLA"
     gene            1961..3778
                     /locus_tag="PLZ72_14695"
     CDS             1961..3778
                     /locus_tag="PLZ72_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37594.1"
                     /translation="MTADMTDYDPVAAVTTQLADGGYRVTRSAGGWIAQCPAHDDRNP
                     SLSIAAGADGRLLLHCHAGCGIRDILDALGLPPAAMFTDYQPDRPGTVTHLNHVRAWR
                     RPQPAAAPRPKPHRREKTHHWDYHDRHGAIVARVVRYNLVDVDTGEIAGKTFTQHAWA
                     DGRQQPNLGGLDMPLYRLPTVLAAAMQDRPVYVVEGERDADTLAALGLTATTCPMGAG
                     SWRPWHTEALSGAADVRIIADNDEPGLAHARHIADELGAAATILLPADGCKDATEHFD
                     AGHTLDDLVMLGEPDPAAAVRDAFPALDWHALWADDSEEEWIVEPILPARRLVALYSP
                     PKVGKSLLLLEIAVAVAQGADVLGTSPDRPRRVLYVDFENDPKLDTRDRLKAMGHTPA
                     DLGNLVVLSFPTMAALDTEAGAIELLAAVAAYECEVVVIDTVSRSIRGEENENDTWLN
                     FYRHTGLALKQAQVSLIRLDHTGKDETKGQRGGSAKSGDVDAVWRMSKVTDTTFKLEC
                     EAARLPITEKTLVIHRETAPVLRHRVDAAGVMAVKRTKVDDLVEWLDANGLPDAASNR
                     EIRDLLAQRGRKVRKDISEEACRVRHGRIRAWEPGDERA"
     gene            4025..4393
                     /locus_tag="PLZ72_14700"
     CDS             4025..4393
                     /locus_tag="PLZ72_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37595.1"
                     /translation="MTTHNPGLFDADHDPRAGVGACRDCGTPVRKVWDDDICALQATV
                     SLHPVDRAVALAAIATGRGAVVHIDARRHSYWRRLDAGNLSSANLAAGRHHLDHACGK
                     DPEEPPPPRPAYTVPDQPPF"
     gene            4402..4590
                     /locus_tag="PLZ72_14705"
     CDS             4402..4590
                     /locus_tag="PLZ72_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37596.1"
                     /translation="MLIYAEATADAITVTATVTATDPATGDSVTLTTRGPGAAAIVRD
                     IPRWWPDALDRIVEELGA"
BASE COUNT          778 a         1855 c         1432 g          552 t
ORIGIN      
        1 ccgccgccga gctcatcgcg gcgtggggcg agcacaggga cgacgaccga tgagcgcccc
       61 cggccactgc cgccactgcc gagacggctg cactctgggc gacacatgcg agttccgcca
      121 tgtgaatgag aactggaccg cggcatggga tgacgagtgc tgccccgcgt gcgacgaggc
      181 cgagcggatc gcgaagacga tcaagcggga agcgatggac gttctagaag cagtagacat
      241 cgccaggggc gaccgatgag ccgcccggag gagccacagg agacgggcag catcgtctgc
      301 gtgcagggga cgttcttcca acgcaccggc gacggtcccg gcgcagtgtg gcaggacatg
      361 acctgggacg ggatgttcct gctgacgtgg aaccagatcg ccccggacgc ccccggcgtc
      421 tgcgtggaga tcgtccgatg agcgcccccg gctgctgccg tcactgcgcg gacatgtgcg
      481 ccgcacgtct gggcgcggtg aacgccgtac atctaggacg gcactcccag tggggagagg
      541 aagcgtgccc cgcctgcgac gaggctgagc gcatcgccgc caagtgcatt gaatgggccg
      601 aaggctgcta ccagcgggga gaggacgacg ccgttcggga actgaacgag atggccgact
      661 ttgcaagggg cgaccgatga cgttcacacc catcggcctg caagcctccg acatcgccgc
      721 gctcatcgcg accgactccg cgcagcgccc gcgcagccgc caggccgcac ccggcccgtc
      781 atccctcggg cacccctgcc ccagacacct cgcccacctg cgcgccggga cacccaagac
      841 gaccggcggc ggcgaccccc tgccgtcatg ggtcgggacc gaggcccacg ccggcatggc
      901 ccgcatcctc gacgggctgc ccgactggga ggccgagata cccgtcgagc tgcccggcta
      961 cggcatccgc ggcacctgcg acgcctacca ccgccccagc cgcctcgtgc tcgactggaa
     1021 gttcgtcggc gccgccgcca tcggcgacta caaggccaac ggccccggcc cccagtaccg
     1081 cacccaggtc cacctctacg gcctcgcgct cgcgctcacc ggccgcgacg tcgaccgcgt
     1141 cggcatcgcg ttcatccccc gcgccgggct gtcctccggc atccacctgt gggccgagga
     1201 cctcgacgag caggtatgcg acgacgcgct gcgccgctgg gacgccatcg tccaggtcgc
     1261 cgacaccctc gggcccgcag cagcccccac gtccggcgac gcccgctgcg actggtgccc
     1321 ctactacgac cccggcggca ccgacccggc cacatcctgc gccggccacc aacccccgac
     1381 cgtcaccggg accgtgacgg aacccacgaa tggaaaggca cgaccatgag cacgttcacg
     1441 tcccccgcag ccgcatccgc aggcatcacc tacgccgacc acctcgggca cctcctcatc
     1501 atcgaagtac tgcgctacga gccgaccgtc gcgaccagcc tcggagacaa ggacgcgatc
     1561 ggcgcgaccg tccacgacgt cgacctcggg accaccgccg aggatgcgct gctgttcccc
     1621 aaagtcctcg tcgcatcgtt gaagaaccgt gtcgggcaga ccgtcctcgc gaccctcacc
     1681 cagggcgtcg ccaagcccgg acagtccgcc ccgtggaccc tgaccgacgc gaccggcgac
     1741 ccggccgccg tcaagcgcgc caccgacttc ctcgacgcct acaagtccgg cgggctggcc
     1801 gcacccgaac cggccgtgga cgcggctacc gccgccgcgc tcaagctgct gaaggaccag
     1861 ggactcgcct gaccaggcag ccctgccccg ccacgggcgg caccggatca cgaccggagc
     1921 agggcaccaa caaaccagca cgacgtcgag gagagcacgt gtgacagcag acatgaccga
     1981 ctacgacccc gtcgcagccg tcaccacgca gctcgcagac ggcggctacc gcgtcacccg
     2041 ctccgccggc ggctggatcg cgcaatgccc cgcccacgac gaccgcaacc cgtcgctgtc
     2101 catcgccgcc ggtgccgacg ggcggctcct cctgcactgc cacgccggct gcggcatccg
     2161 cgacatcctc gacgccctcg gcctgccgcc cgccgccatg ttcaccgact accagcccga
     2221 ccggcccggc accgtcaccc acctcaacca cgtccgcgcc tggcgccgcc cccaacccgc
     2281 cgccgcaccc agacccaaac cccaccgccg cgagaagacc caccactggg actaccacga
     2341 ccgccacggc gccatcgtcg cccgcgtcgt gcgctacaac ctcgtcgacg tcgacaccgg
     2401 cgagatcgcc ggcaagacgt tcacgcagca cgcctgggcc gacgggcgcc agcagcccaa
     2461 cctcggcggc ctggatatgc cgctgtaccg actgcccacc gtcctcgcgg ccgccatgca
     2521 ggaccggccc gtctacgtcg tcgagggtga gcgcgacgcc gacaccctcg ccgcgctcgg
     2581 gctcaccgcc accacctgtc ccatgggcgc cgggtcgtgg cggccctggc acaccgaagc
     2641 cctgtccggg gccgcggacg tccgcatcat cgccgacaac gacgagccag gcctcgccca
     2701 cgcccgccac atcgccgacg agctcggcgc cgccgccacc atcctgctgc ccgccgacgg
     2761 gtgcaaggac gccaccgagc acttcgacgc cgggcacacc ctcgacgacc tcgtcatgct
     2821 cggcgagccc gacccggccg ccgccgtgcg cgacgcgttc cccgcgctcg actggcacgc
     2881 cctgtgggcc gacgactccg aggaggaatg gatcgtcgag ccgatcctcc ccgcccgccg
     2941 cctcgtcgcc ctctactccc cgccgaaggt cggcaagtcg ctgctgctgc tggagatcgc
     3001 cgtcgccgtc gcccagggcg ccgacgtcct cggcaccagc cccgaccgcc cgcgccgcgt
     3061 gctctacgtc gacttcgaga acgacccgaa gctcgacacc cgcgaccggc tcaaagccat
     3121 gggccacacc cccgccgacc tcggaaacct cgtcgtcctg tcgttcccga cgatggccgc
     3181 gctggacacc gaagccggcg ccatcgaact cctcgccgcc gtggccgcgt acgagtgcga
     3241 ggtcgtcgtc atcgacaccg tgtcgcgatc catccgcggc gaggagaacg agaacgacac
     3301 atggctcaac ttctaccggc acaccgggct cgcgctcaag caggcccagg tgtccctcat
     3361 ccggctcgac cacaccggca aggacgagac gaaggggcag cgcggcggct cggcgaagtc
     3421 cggcgacgtc gacgccgtgt ggcgcatgtc caaggtgacc gacacgacgt tcaagctcga
     3481 gtgcgaagcg gcgcggctac ccatcaccga gaagacgctg gtgatccacc gcgagaccgc
     3541 gccggtgctg cggcaccggg tcgacgccgc cggcgtcatg gccgtcaagc gaacgaaggt
     3601 agacgacctc gtcgaatggc tggatgccaa cgggctaccc gacgcggcga gtaatcggga
     3661 gatccgcgac ctcctcgcgc agcgggggcg caaggttcgc aaggacatca gcgaggaggc
     3721 gtgccgggtg cgacatggcc gtattcgagc ctgggaaccg ggtgatgaac gtgcctgaga
     3781 cggccctgaa agcgtccccc gatcggccgg ggacacctac ccctgaattg gtgtcccccg
     3841 gggacacttg gggacgcttg gggacgcttg tcaataatca agtaaatatg ctggtcagag
     3901 catgtttcag gtgtcccccg caaggccggg gacacctgtc cattagcgca cctgttgggg
     3961 gtgtcccccg ctacccgcta cctagtagcg gggacaccac cgacgcttcg gggacgggca
     4021 cgttatgacg acccataacc cgggactctt cgacgccgac cacgaccccc gcgccggcgt
     4081 cggtgcctgc cgcgactgtg gaacccccgt ccgcaaagtg tgggacgacg acatctgcgc
     4141 actccaggcc accgtgtcgc tccaccccgt cgaccgggcc gtcgcgctcg ccgcgatcgc
     4201 caccggccgc ggcgccgtcg tccacatcga cgccaggcgg cattcctact ggcgccggtt
     4261 ggacgccggc aacctgtcct cggccaacct cgccgccggg cggcaccacc tcgaccacgc
     4321 ctgcggcaaa gaccccgagg agcccccgcc gccccgaccc gcctacaccg tccccgacca
     4381 gcccccgttc taggagaagc cgtgctcatc tacgccgagg ccaccgccga cgccatcacc
     4441 gtcaccgcca ccgtcaccgc caccgacccg gccaccgggg actccgtcac gctcaccacc
     4501 cgcggccccg gcgccgccgc catcgtccgc gacatcccgc gctggtggcc cgacgcgctc
     4561 gaccgcatcg tcgaggagct cggcgcgtga gcgccctgtg ccccgggccg cacgccg
//
LOCUS       DAOQOD010000220         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8051_length_4616_cov_10.350800, whole genome shotgun sequence.
ACCESSION   DAOQOD010000220 DAOQOD010000000
VERSION     DAOQOD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_4616_cov_10.350800"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLZ72_14710"
     CDS             <1..1275
                     /locus_tag="PLZ72_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNA37597.1"
                     /translation="DGARRAAEAIDARVAAGEADLLGPLAGVPFGVKDLEDCEGMPTS
                     HGSVLYQGGPPVAADSTHVARLRAAGAIPIGKTAAPEFGTLQYTYTKAWGATGNPWDP
                     ERTPGGSSGGTAAAVAAGMIPFGTASDGGGSTRIPAGFTGLVGLKPSHGRIPHPDVDP
                     SETACYGALTTTVSDSARHLDVTAGPHDTDRLSLPPPPMRYEDAIDTLDVSGLRVGWS
                     ADLGFAQVDAEVAEVSRAAALDVAAAAGVELVDVDVRLTDPVATWLSAGAMSLWLSIM
                     EHEHWPDRADDLTPYVRTALEATYDRPLRTLVNGYRRRLQLQQDMSDLFAEVDVLMTP
                     TTAVPAFSAKGPPPEEIAGVAVGAAMSVPFTMLANLCWNPAVSVPAGLNSEGLPIGLQ
                     VIGRRHHDEVPLRFARLLELARPWPRHAPAPG"
     gene            1287..2423
                     /locus_tag="PLZ72_14715"
     CDS             1287..2423
                     /locus_tag="PLZ72_14715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNA37598.1"
                     /translation="MDLVVYTDAAERGGAESSMGMFIERFRPDIRVTVVGPHEPVIRW
                     LASRRPGSRWMVLDEIRDRSDVAAMVQHRRAFRWLDPDLVHFNLSTMSSCQWALAAAL
                     SIPGLPVVVLEHSPVGTWSGLSNRLKRITSRRADAHVAVGHAAARLIEQLGDLPPGSM
                     TVIHSGVPIEELDPPARTDDRFTIGMLSRHDPVKGIDLAIRAVERLGPDVRLVVVGDG
                     AERPALEALVEQLGVGDRVELDGWDDRARHRLAGVDLYLLPSRLEAFPVTVMEAMQAG
                     VPVVATDVGSVREAVDDGETGIVVPAPAPLSDHVAGTAAAARADEVTVAAIVDAVTRL
                     RDDPEARRRMGDRARVVGRDRFDAAAGVAAWEQLYDRVLAGRRG"
     gene            complement(2446..3180)
                     /locus_tag="PLZ72_14720"
     CDS             complement(2446..3180)
                     /locus_tag="PLZ72_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37599.1"
                     /translation="MTKDTHFPPPSRPDARFDAIVDRGRSIRRKDRMRRALVTAGTVA
                     LVLLVIGVGVIGATRGERRVDPVTPPTTAPSTEMEVTATAEHDAVVVDVDDPRAAAGD
                     RTGACVLVRIQPEEPARVATSEAYACWNPTLGSAATVVAMSPATAEVGCATTLERDPA
                     DDGSPTTEASGTEPSGNVPGTAAPPSGPTGELHHTFRFTLPTGLPAGTYVAEVQGSTG
                     PGEPCPTAVLPAGAHRASHSTAVEVP"
     gene            complement(3177..3689)
                     /locus_tag="PLZ72_14725"
     CDS             complement(3177..3689)
                     /locus_tag="PLZ72_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HNA37600.1"
                     /translation="MPVPVRDDEFRAFYERMHDRALGTARRLLGDRTVAEDISAEALA
                     RAYARWEHVRSHPNPDAWLLRVVGNLAIDHRRRDRSGPMPRLEPAAPDRPDDAAALQV
                     DLARAVHRLSRRQQEVVVMRYLIDLSEEDVAGSLGMSEGSVKTHLSRARGHLREHMIE
                     LDRIGSGVDR"
     gene            3867..4481
                     /locus_tag="PLZ72_14730"
     CDS             3867..4481
                     /locus_tag="PLZ72_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37601.1"
                     /translation="MTVFDALFWVVSLVLVVAGASKLTDPAPVAATLSALRRPSGSTA
                     HPAGPGSGVGLARTVGALEVLVGLAALSVGGPLVAFAVAAAYAAFAVVVVVARSRGLE
                     SCGCFGVASAPPSWVHVAVNVVSAAVAVAAAVAGPVPASDGLSALGGPDAVVVGCLVL
                     LATALVVVIDTTVADVVEAARLVRSQADDDPTPSPERPSEVLTP"
BASE COUNT          542 a         1701 c         1722 g          651 t
ORIGIN      
        1 gacggcgccc gtcgcgccgc cgaggcgatc gacgcccggg tcgccgccgg tgaggccgac
       61 ctgctcgggc cgctggccgg cgtcccgttc ggggtgaagg acctcgagga ctgcgagggc
      121 atgccgacca gccacgggtc ggtgctctac cagggcggcc cgcccgtcgc cgcggactcg
      181 acccacgtcg cccgcctgcg cgccgccggc gccatcccga tcggcaagac cgccgctccc
      241 gagttcggga cgttgcagta cacgtacacc aaggcgtggg gggccaccgg caacccgtgg
      301 gaccccgagc gcaccccggg cggctcgagc ggggggaccg cggccgcggt ggccgccggg
      361 atgatcccgt tcggcaccgc gtccgacggc ggcggctcca cccgcatccc cgccgggttc
      421 accggcctgg tcggactcaa gcccagccac ggccgcatcc cgcaccccga cgtcgacccg
      481 tccgagaccg cctgctacgg cgcgttgacc accaccgtct ccgacagcgc ccgccacctc
      541 gacgtcaccg ccggtcccca cgacaccgac cgactgtcgc tgccgccgcc ccccatgcgg
      601 tacgaggacg cgatcgacac cctcgacgtg tcgggcctgc gggtggggtg gtcagcggat
      661 ctcggcttcg ctcaggtcga cgccgaggtg gccgaggtgt cgcgggccgc agcgctcgac
      721 gtggccgccg ccgccggagt ggagctcgtc gacgtcgacg tccggctgac cgacccggtg
      781 gccacgtggc tgtcggccgg cgccatgtcg ctgtggctgt cgatcatgga gcacgagcac
      841 tggccggacc gggccgacga cctgaccccg tacgtgcgca ccgcgctcga ggccacctac
      901 gaccggccgc tgcgcacgct ggtcaacggg taccgccgca ggttgcagct ccagcaggac
      961 atgtcggacc tgttcgccga ggtcgacgtg ctgatgacgc cgaccaccgc cgttccggcg
     1021 ttctcggcca agggtccgcc gcccgaggag atcgccggcg tggcggtcgg cgcggccatg
     1081 agcgtcccgt tcacgatgct cgccaacctg tgctggaacc cggcggtgtc ggtgcccgcc
     1141 ggcctcaact ccgaggggct gccgatcggg ttgcaggtga tcggtcgccg ccaccacgac
     1201 gaggtgccgc tgcgcttcgc ccgtctgctc gagctggccc gcccctggcc gcgtcacgcc
     1261 cccgcccccg gctgaccggc acgtccgtgg acctggtcgt ctacaccgac gcggccgagc
     1321 gcggcggggc cgagtcgtcg atggggatgt tcatcgagcg gttccgtccc gacatccgcg
     1381 tcaccgtcgt cggcccccac gaaccggtca tccggtggtt ggcatcgcgg cgcccgggtt
     1441 cgcgttggat ggtcctcgac gagatccgcg atcgctccga cgtcgccgcc atggtgcagc
     1501 accgccgggc gttccgctgg ctggacccgg acctggtgca cttcaacctg tcgaccatgt
     1561 cgagctgcca gtgggcgctg gccgcagcgc tcagcatccc gggcctgccg gtggtcgtgc
     1621 tcgagcacag cccggtcggc acctggtcgg gtctgtccaa ccggctcaag cgcatcacgt
     1681 cacggcgggc cgacgcccac gtcgcggtcg gtcacgccgc ggcccggctg atcgagcagc
     1741 tcggcgacct gccgccgggg tcgatgaccg tcatccactc cggcgtgccg atcgaggagc
     1801 tcgacccgcc ggcgcgcacc gacgaccgct tcaccatcgg catgctcagc cgtcacgacc
     1861 cggtcaaggg catcgacctg gcgatccggg ccgtcgagcg gctcgggccc gacgtgcgcc
     1921 tggtcgtcgt cggcgacggg gccgaacgcc cggcactcga agcgctcgtc gagcagctcg
     1981 gcgtcgggga ccgggtcgag ctcgacggct gggacgaccg cgcccgtcac cggctggccg
     2041 gcgtcgacct ctacctgttg ccctcacgac tcgaggcgtt cccggtgacg gtgatggagg
     2101 cgatgcaggc cggggtgccc gtggtcgcca ccgacgtcgg cagcgtgcgc gaggcggtcg
     2161 acgacggcga gaccggcatc gtggttcctg ctccggcccc gctgtcggac cacgtcgcgg
     2221 gcaccgccgc cgcggcccgc gccgacgagg tgaccgtggc ggcgatcgtc gacgccgtga
     2281 cccggttgcg tgacgacccg gaggcccgac ggcgcatggg tgaccgggcc cgggtcgtgg
     2341 gtcgggatcg cttcgacgcc gcggccggcg tcgcggcatg ggagcagctc tacgaccggg
     2401 tgctggccgg gcgacgaggt tgagcggcgg ccgggctgtc gggcctcagg ggacctcgac
     2461 cgccgtcgag tgcgaggcgc ggtgggcgcc ggcggggagc acggcggtcg ggcacggttc
     2521 gcccggaccg gtgctgccct ggacctcggc cacgtaggtg cccgcgggca gcccggtggg
     2581 cagggtgaag cggaaggtgt ggtgcagctc accggtcgga cccgacgggg gcgcggcggt
     2641 gcccggcacg ttgccggacg gctcggtacc ggacgcctcg gtggtgggcg atccgtcgtc
     2701 tgccgggtca cgctcgagtg tggtggcgca gccgacctcg gccgtcgccg gcgacatagc
     2761 caccacggtg gcggcgctgc cgagggtggg gttccagcag gcgtaggcct cggaggtggc
     2821 cacacgggcc ggttcctcgg gctggatgcg gacgagcacg cacgctccgg tgcggtcacc
     2881 cgcggctgct cggggatcgt cgacgtcgac cacgaccgcg tcgtgctccg cggtcgccgt
     2941 gacctccatc tcggtggacg gggcggtggt cggcggtgtc accggatcga cccgacgttc
     3001 gccgcgggtg gcgccgatga cgccgactcc gatgacgagc agcacgagcg cgacggtgcc
     3061 ggcggtgacg agcgcccggc gcatgcggtc cttccgtcgg atgctccgac cccggtccac
     3121 gatcgcgtcg aaacgggcat cgggccgcga cggaggcggg aagtgggtgt ccttggtcat
     3181 cggtcgaccc ccgatccgat ccggtccagt tcgatcatgt gctcccgcag atgcccccgg
     3241 gcccgcgaca ggtgggtctt gaccgagccc tccgacatgc cgagcgagcc ggcgacgtcc
     3301 tcctcgctca ggtcgatcag gtagcgcatc acgaccacct cctgctggcg gcgcgacagc
     3361 cggtgcaccg cccgggccag gtcgacctgc agcgcggcag cgtcatcggg acggtccggg
     3421 gcggccggct cgagccgggg catgggaccg ctgcggtcgc ggcgtcggtg gtcgatggcc
     3481 aggttgccga ccacccgcag caaccaggcg tcggggttgg ggtgcgaacg cacgtgctcc
     3541 cagcgggcgt aggcccgggc gagcgcctcg gcggagatgt cctcggccac ggtgcggtcg
     3601 ccgagcagac gtcgggccgt gcccagggcg cggtcgtgca tccgttcgta gaacgcccgg
     3661 aactcgtcgt cgcgcacggg aactggcaac acaccctcct acgacgtccg aacgtaccga
     3721 agggttgaca ccggggtgaa cccgccgggc ggatctcccc agtcggatct gccagttcga
     3781 cgcgacatcg gagcggtccg gaccggcccg cttcgcgtcg ctcaggacgg gctcgtcggc
     3841 ggtgcgtggc gattaccctg tcggccgtga cggtcttcga cgcgctcttc tgggtggtca
     3901 gcctggtgct ggtcgtcgcc ggtgcgtcca agctgaccga ccccgcgccg gtggcggcga
     3961 cgctgtcggc gctgcgacgg ccgagtgggt cgacggcgca cccggccggt ccgggatccg
     4021 gcgtcgggct cgcccggacc gtcggggcgc tggaggtcct cgtcgggctg gcggctctca
     4081 gcgtcggggg cccgctcgtc gcgttcgccg tcgcggcggc ctacgccgcg ttcgccgtgg
     4141 tggtcgtcgt ggcccgttcc cgtggcctgg agtcctgtgg gtgcttcggc gtggccagcg
     4201 ctccgccgag ctgggtgcac gtcgcggtca acgtcgtgtc ggccgcggtc gcggtcgcgg
     4261 ctgcggtcgc cggtcccgtc cccgcctcgg acggcctgtc cgctctgggc ggacccgatg
     4321 ccgtcgtcgt cggatgcctg gtgctgcttg ccaccgcgct ggtggtggtg atcgacacca
     4381 ccgtcgctga cgtggtcgag gcggcccggc tggtccgctc ccaggccgac gacgatccca
     4441 caccgtcgcc ggagcgtccc tcggaggtcc tgacgccgtg accatcagcc caccgcggga
     4501 caccgacccg atgaacgtgc tgggccgcgc tcggtcggcc ccgtcgctgt cgaccgacac
     4561 cccggcgtcc gacggagcgg gacgcaccga ctccggagcc cgccccgctg ccgggc
//
LOCUS       DAOQOD010000221         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8181_length_4571_cov_11.594996, whole genome shotgun sequence.
ACCESSION   DAOQOD010000221 DAOQOD010000000
VERSION     DAOQOD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_4571_cov_11.594996"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1215
                     /locus_tag="PLZ72_14735"
     CDS             40..1215
                     /locus_tag="PLZ72_14735"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNA37602.1"
                     /translation="MRFAPDRPLGLAPATIGDYRELARRRLPRQLFDYIDGGAYDEST
                     MRDNVADLERIRLRQRVMVDVDTRSTATTVLGEDLALPVILGPVGLAGMFAPRAEVQA
                     IRAATAAGVPFVESTVSICGLDEVARASVKPPWFQLYVMRDRDFAEGLMQQAGDVGSP
                     VLVLTVDLAVVGARFRDVRNAMVGDVGPGGAIRRGLDLASHPRWIKEVALGGKPLTFG
                     NLESAVPGARTPDSFKSWVDAQFDPTVTWDDVSWVRRNWSGKLVVKGVLDADDARRAV
                     DAGVDGVVVSNHGGRQLDGVPSTVAALPAVLDAVGDRTEVLVDGGVRSGLDVVRFLAL
                     GARACLVGRAWAWAVAARGEAGVAHVLDVLGSDMDVALALTGTTDVADLDRSILIGA"
     gene            complement(1252..2118)
                     /locus_tag="PLZ72_14740"
     CDS             complement(1252..2118)
                     /locus_tag="PLZ72_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37603.1"
                     /translation="MLGTRPIQRSRAATAVAAIAVLLGTTLVGCSPTPAPPSSRCSTD
                     ALVLTCPHNSTTLVAAGEARVVHWQVPRGTPPTGGWPVAIMFQGTLATAALSWTASPL
                     EPFGAYYQTQVVQGLLDNGFAVLTPETHLAGLTFWDTNNPLVPDYYQSNDHALMLTIF
                     DRLADGTFGAVNPARLFATGISSGGYMTSRMAVSYPGRFKALAIQSASYATCAGPLCS
                     IGPIPADHPPTLFLHGNLDPVVPVGTMWAYAGALNAAGRPTRVVVDPLSLHRWIPQAP
                     GEVVNWFRTYNS"
     gene            2207..2833
                     /locus_tag="PLZ72_14745"
     CDS             2207..2833
                     /locus_tag="PLZ72_14745"
                     /inference="COORDINATES: protein motif:HMM:NF037009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNA37604.1"
                     /translation="MARRPRQDRSEETRHRILDAVVELIAERGLAGVSHRAVAERAGV
                     SLSATSYYFGSKAELERAALEEHYRRRLDDYRRTAELLAGADSSADELVDAAVELFTA
                     SDLTMLLSHFEVFLNSARRDDIRDLLAPTLDAMHDLLLVAIEPLRLPDPEAAATAITA
                     VIEGVELRRVALGIDGRDELRGSLRALLRGLVRPDGEVGPERAAPSGE"
     gene            2860..4137
                     /locus_tag="PLZ72_14750"
     CDS             2860..4137
                     /locus_tag="PLZ72_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNA37605.1"
                     /translation="MTQTSPRPHGTRWTFRDGLESTRLRAGLAVPVLIVGVGGGLIGA
                     AYLAALRLVTEWIGPGSRAALGQTAIMVTVGIAIALITRLLGDSGNVELLVDNIHVLG
                     GAEDLRQIRPLIPQSLLCVGAGGAMGPEAPLVQTNGAFGTWVAGRFRLDAHDMRVLTI
                     TGMAGGFSILFGAPLGAALFALEILHKRGLQYYEALVPALVGALIGFALNFGLTGLGL
                     EPVWQLPAVGELTGGDLLWGVACGVIGAGGALLFAAAARLGHWAVSRVPVAVLPAVGG
                     ALLGALYWWSPFALTNGEMQVESIVTGGLTVGALAVAIVAKFAGVIVTLSGRWKGGFI
                     IPLFFIGIAGGQLIHLLVPSTNATVLMVALAVALCVGVTKTPLGSTLVVAQMAGLALV
                     PTALAASVIALVLTNRSTVIESQRARAPSEEKR"
     gene            4134..4559
                     /locus_tag="PLZ72_14755"
     CDS             4134..4559
                     /locus_tag="PLZ72_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNA37606.1"
                     /translation="MSPSPGGPDLPDEDYRRLASFRHALRQFLRFSEQAARAEGLTPN
                     QHQLMLAVRGWSGPDSPTITELADQLQLKVHSTSELVQRAVVSGMVEVAVDTEDHRRQ
                     HVELTPEGDDKLRSLSVLHRDELRRFRTHLADLVDAFDG"
BASE COUNT          590 a         1560 c         1716 g          705 t
ORIGIN      
        1 ggggcgcacg ggcccgacgg gctgcctagc ctcgcggcca tgcgattcgc tcccgatcga
       61 cccctcggcc tggccccggc caccatcggc gactaccgcg agctcgcccg tcgtcgcctt
      121 ccccgccagc tcttcgacta catcgacggc ggcgcctacg acgagtcgac catgcgcgac
      181 aacgtggccg atctggagcg gatccggctg cgccagcggg tgatggtcga cgtcgacacc
      241 cggagcaccg cgacgacggt tctgggtgag gatctggcgc tgccggtcat cctcggaccc
      301 gtcggactcg ccggcatgtt cgctccccgc gccgaggtgc aggcgatccg tgccgccact
      361 gcggccgggg tgccgttcgt cgagtccacc gtgtcgatct gcgggctcga cgaggtggcc
      421 cgggcgtcgg tgaagcctcc gtggttccag ctctacgtca tgcgcgaccg cgacttcgcc
      481 gagggtctga tgcaacaggc cggagatgtc gggtcaccgg tcctggtcct caccgtcgac
      541 ctggcggtgg tcggtgcacg attccgcgac gtccgcaacg ccatggtcgg cgacgtcggc
      601 ccggggggag cgatccgtcg gggcctggac ctggcgtcgc acccgcgttg gatcaaggag
      661 gtcgccctcg gcggcaagcc gctcacgttc ggcaacctcg agtcggcggt gccgggggca
      721 cgcactccgg actcgttcaa gtcgtgggtc gacgcccagt tcgatccgac tgtcacctgg
      781 gacgacgtct cgtgggtgcg ccggaactgg tcgggcaagc tcgtggtcaa gggcgtgctc
      841 gatgccgacg acgcccggcg ggcagtcgac gccggtgtgg acggcgtggt ggtgtccaac
      901 catggaggcc gccagctcga cggcgtgccg tcgacggtcg cggcccttcc ggcggtcctg
      961 gatgcggtgg gggaccggac cgaggtgctc gtcgacggcg gcgtccgatc cgggctcgac
     1021 gtcgtgaggt tcctggccct cggggcgagg gcgtgcctgg tcggccgggc ctgggcgtgg
     1081 gcggtcgctg ctcggggcga ggccggcgtg gcccacgtgc tcgacgtcct gggctccgac
     1141 atggacgtcg cgctggcgct gaccggtacc accgacgtcg ccgacctgga ccggtcgatc
     1201 ctcatcgggg cctgaccctc ggtcgcctgg acgggcggtg tgggggacgg ttcaggagtt
     1261 gtaggtccgg aaccagttca cgacttcgcc cggggcctgc gggatccaac ggtgcaacga
     1321 cagcgggtcg accacgaccc gggtcgggcg gcccgccgcg ttcagtgcgc cggcgtaggc
     1381 ccacatcgtt ccgaccggga cgaccgggtc gaggttcccg tgcaggaaca gagtcggcgg
     1441 gtggtccgcc gggatggggc cgatcgagca gagcggcccc gcgcaggtcg cgtacgacgc
     1501 cgactggatg gccagcgcct tgaagcggcc ggggtacgac accgccatcc ggctggtcat
     1561 gtaacccccg ctggagatcc cggtggcgaa cagccgggcc gggttcaccg ctccgaaggt
     1621 cccgtccgcc agccggtcga agatcgtgag catcagggcg tggtcgttgg actggtagta
     1681 gtcgggaacc agcgggttgt tcgtgtccca gaacgtgagc ccggccaggt gcgtctcggg
     1741 ggtgagcacg gcgaagccgt tgtcgaggag cccctggacg acctgcgtct ggtagtaggc
     1801 gccgaacggc tcgagcggcg acgccgtcca gctcagcgcg gcggtggcga gggtgccctg
     1861 gaacatgatg gccaccggcc acccgccggt gggcggggtg ccgcggggta cctgccagtg
     1921 cacgacccgg gcctcgccgg cggcgacgag cgtggtcgag ttgtgcgggc aggtgagcac
     1981 gagcgcatcg gtgctgcagc gagagctcgg cggggccggg gtgggcgagc agcccacgag
     2041 ggtcgtgccc aacaggacgg cgatggccgc gacggcggtc gcggcccggc tccgctggat
     2101 cggtcgcgtt cccaacatgg acaccccccg gtggtcggtt cgacgggacg accgtaccat
     2161 gaaacaggac gatcgtccta gaaccacggc ttgctatcgt ccgaccgtgg cccgccgtcc
     2221 ccgacaggac cgcagcgagg agacccggca ccggatcctc gacgcggtgg tcgagctcat
     2281 cgcggagcgg ggcctggccg gcgtgtcgca ccgggcagtg gcggagcggg cgggagtgag
     2341 cctgtcggcg accagctact acttcggctc caaggccgag ctcgagcggg ccgcgctgga
     2401 ggagcactac cggcgccgcc tcgacgacta ccggcggacc gcggagctgc tcgccggtgc
     2461 ggactcgtcg gccgacgagc tggtggacgc cgcggtcgag ctgttcaccg ccagcgacct
     2521 gaccatgctg ctcagccact tcgaggtctt cctcaactcg gcccgccgcg acgacatcag
     2581 ggatctactg gcgccgaccc tcgacgccat gcacgacctg ttgctcgtgg cgatcgagcc
     2641 gttgaggctg cccgaccccg aggccgcggc gacggccatc accgcggtga tcgaaggcgt
     2701 cgagctgcgt cgggtggcgc tgggcatcga cggacgtgac gagctccggg ggtcgctccg
     2761 ggccctgctg cggggcctgg tccggcccga tggtgaggtc ggcccggaac gagcggcacc
     2821 gtctggtgaa tagttcgtag tgcgatctat cgtggaggcg tgacccagac ctcgccccgg
     2881 ccccacggga cccgctggac gttccgcgac ggcctcgaga gcacacggct ccgcgccggt
     2941 ctggccgtgc cggtgctcat cgtcggcgtg ggcggcgggc tgatcggcgc tgcctacctg
     3001 gccgcgctgc gactggtgac cgagtggatc ggccccggct cgcgggcggc tctcggccag
     3061 acggccatca tggtgaccgt cgggatcgcc atcgccctga tcacccggct cctgggcgac
     3121 agcggcaacg tcgagctgct ggtcgacaac atccacgtgc tcggaggcgc cgaggacctg
     3181 cgtcagatcc gccccctcat cccccagtcc ctgctgtgcg tcggtgcggg aggggcgatg
     3241 ggccccgagg ccccgctggt ccagaccaac ggcgccttcg ggacctgggt cgccggtcgg
     3301 ttccgcctcg acgcccacga catgcgggtc ctgaccatca ccggcatggc cggcgggttc
     3361 agcatcctgt tcggcgcgcc gctgggcgct gcgttgttcg ccctggagat cctccacaag
     3421 cgggggctgc agtactacga ggcgctggtt ccggcgctgg tcggcgccct gatcggcttc
     3481 gccctcaact tcgggctgac cgggctgggc ctggagccgg tgtggcagct gccggcggtg
     3541 ggagagctca ccggtggcga cctgctctgg ggtgtggcgt gcggggtcat cggtgccggc
     3601 ggtgccctcc tgttcgccgc ggcggcccgg ctggggcact gggcggtgag tcgggtgccg
     3661 gtggcggtcc tgccggcggt cggcggggcg ctgctcgggg ccctctactg gtggtcaccc
     3721 ttcgctctga ccaacggcga gatgcaggtc gagtcgatcg tgacgggggg cctgaccgtg
     3781 ggcgcgctcg ccgtcgccat cgtcgcgaag ttcgccggtg tgatcgtgac cctcagcgga
     3841 cggtggaagg gcggcttcat catcccgctg ttcttcatcg gcatcgccgg cggccaactg
     3901 atccacctcc tggtgccgag cacgaacgcg accgtgctca tggtggcgct cgcggtggcg
     3961 ctgtgcgtcg gggtgaccaa gacgccgctc gggtcgacgt tggtggtcgc tcagatggcc
     4021 ggcctggcgc tcgtacccac cgcgttggcg gcctcggtga tcgccctggt gctcaccaac
     4081 cggtcgacgg tcatcgagtc gcaacgggcc cgcgccccga gcgaggagaa gcgatgagtc
     4141 cgtccccggg cgggcccgac ctgcccgacg aggactaccg gcggctcgct tcgttccgcc
     4201 acgcactccg gcagttcctg cgcttctcgg agcaggcggc gcgggccgag gggctcaccc
     4261 ccaaccagca ccagctcatg ctggcggtcc ggggatggtc cggcccggac agcccgacga
     4321 tcaccgagtt ggccgaccag ctccagctca aggtgcactc gaccagtgag ctcgtccagc
     4381 gggcggtggt gtcggggatg gtcgaggtgg ccgtcgacac cgaggaccat cggcgtcagc
     4441 acgtcgagct gacccccgag ggtgatgaca agctgcggtc gctgtcggtg ctgcaccgtg
     4501 acgagctgcg ccggttccgc acccacctgg cggacctggt cgacgcgttc gacggctgac
     4561 ggtgccgtcg g
//
LOCUS       DAOQOD010000222         4525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8342_length_4525_cov_11.331544, whole genome shotgun sequence.
ACCESSION   DAOQOD010000222 DAOQOD010000000
VERSION     DAOQOD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_4525_cov_11.331544"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLZ72_14760"
     CDS             <1..1362
                     /locus_tag="PLZ72_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNA37607.1"
                     /translation="GAGTPGGPAARPAQPAPAPRRDPALNELRHRIHQQLIDELGPIL
                     FDRRLSEQDLRRRVHEQLHAAIAAERVPLSAADKAQLIQDVSDDILGYGPIDKLLRDE
                     DVTEVMVNGPDLVFVERSGKLTKDESIRFIDSDHVRRVIDKIVSEVGRRVDESTPMVD
                     ARLPDGSRVNAVISPLSIGGPFLTIRKFATDPLQIDDLIRFGSLNAHSARFLQACIVG
                     KLNVIVAGGTGTGKTTMLNVLSSFIPEDERIITVEDAKELQLHQEHVLCMESRPPNVE
                     GRGEVTIRDLVKNCLRMRPDRIVVGECRSGEALDMLQAMNTGHDGSLTTVHANSPRDT
                     LARLETLVLMAGFDLPVRAIREQMASAIDVIVQLSRLRDGTRRVTHITEVQGMEGDVI
                     TLQDVFLFDFGAGVDENGRYRGQLQATGVRPKFAEKLADQGIRLGPEVFARDGGRKAV
                     TGR"
     gene            1368..3305
                     /locus_tag="PLZ72_14765"
     CDS             1368..3305
                     /locus_tag="PLZ72_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA37608.1"
                     /translation="MRNTRSRLGALMVGLAAAACLFGLVGVVGSAGAAEDPPPSLTVQ
                     KVDARGDEVNAFGNLVGGDAQRLRAKVNGKDVAADVVAGSDLPLDAVVVLDNSASLGN
                     ATVQLAKQSLAPLLPGKGITRSLALVTTGGGASVAIGSTTSAEAFQAALDDVEPVGAS
                     LTWDGLVRAADIIGNDGSAQQNIILFSASPPQVGTATAASAQGAMAQAGAQLNAVVIP
                     RGADLEAIDNMVAGLGGSVTMAADEEGLDEAVDSVAVQLQGRFVLRYPSTGSSSSTTL
                     TVSAGKLSTTVAYVPGTTKSGAANLAPPVSVSPSITERILGNPLGLILIVLIGFGAVA
                     LFIWTLLNMVLPSSDNLNRRLKVYEDPYGESGEEADEPADGSHTTVPIIQRAVEFTGD
                     VAQKRGLLEKLEGDLERANLPLRGAEALFFLAVAVVLMSLLTFALTRSLLPALVVAAA
                     TVLIPRAVLSIAVRRRCRAFEQQLPDTLTLMAGTLRAGYSIGQGFEAVSTEIPDPMGR
                     ELRRVVTETRLGRPLDEALESVAERMKSDDFSWAVMAIRIQREVGGNLAELLVTVAET
                     MTQRERLRRDVATLTAEGRMSAIVLGFLPPALGLVMWAINPAYMQKLMTPGLGYILLG
                     LGLVSMLIGFAWMKKIITIEV"
     gene            3310..4236
                     /locus_tag="PLZ72_14770"
     CDS             3310..4236
                     /locus_tag="PLZ72_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNA37609.1"
                     /translation="MSPILLLSVLLIAGAVGVAVWAVLSQLDERQVVREALRQLDGYE
                     VENQRDKQMLAPLKDRAIGPLVEGVTGLGRRFTPVGYVEKVKQKLVYAGKADQAAVDR
                     FLGIRVVTIGVAVLVFVLLFMFNILGLSGTLRLAVPGLFILALVLGPDSMLNRQVEER
                     QHEIQITLPDVMDLLTISVEAGLGFEQAIDRVVSSVPGALSDEFSRMLGETRAGASRS
                     DAMRAMDDRCNVPELRSFVMAIIQADQFGVSIGRVLRGQADEMRIKRRQLAQERAQKA
                     PVKMLIPMVFCIFPALFVVVLGPAIMNIRESL"
     gene            4287..>4525
                     /locus_tag="PLZ72_14775"
     CDS             4287..>4525
                     /locus_tag="PLZ72_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37610.1"
                     /translation="MFGDAVFEGAVLEGAVAADAPEQDNPDADESTGSAPRRRWPDGG
                     LGVLVAGLLAFVAARPLTDNSFLTHLATGRLILEQG"
BASE COUNT          679 a         1612 c         1547 g          687 t
ORIGIN      
        1 ggtgccggca cacccggtgg cccggccgcc cgacccgccc agccggcccc ggcgccccgc
       61 cgcgaccctg ccctcaacga gctgcgccac cggatccacc agcagctcat cgacgagctc
      121 ggcccgatcc tgttcgaccg gcgcctgtcg gagcaggacc tgcgccgtcg ggtccacgag
      181 cagctccacg ccgccatcgc ggccgagcgc gtgccactgt ccgcggccga caaggcgcag
      241 ctcatccagg acgtcagcga cgacatcctg ggctacgggc cgatcgacaa gctgctgcgt
      301 gacgaggacg tcaccgaggt catggtcaac ggacccgacc tggtgttcgt cgagcgcagc
      361 ggcaagctga ccaaggacga gtcgatccgc ttcatcgact ccgatcacgt gcggagggtg
      421 atcgacaaga tcgtctccga ggtcggacgc cgggtggacg agtcgacccc gatggtcgac
      481 gcccgcctgc ccgacggctc ccgcgtcaac gcggtgatct caccgctgtc gatcggcggc
      541 cccttcctca ccatccggaa gttcgccacc gacccgctgc agatcgacga cctgatccgc
      601 ttcggatcgc tcaacgcgca ttcggcccgc ttcctgcagg cctgcatcgt cggcaagctg
      661 aacgtgatcg tcgccggcgg taccggcacc ggcaagacga ccatgctcaa cgtgctgtcg
      721 tcgttcatcc ccgaggacga gcgcatcatc accgtcgagg acgccaagga gctccagctg
      781 caccaggagc acgtcctgtg catggagtcc cgaccgccca acgtcgaggg ccgcggtgag
      841 gtcaccatcc gcgacctcgt caagaactgc ctgcgcatgc gcccggaccg gatcgtggtc
      901 ggcgagtgcc gttccggcga ggcgctcgac atgctccagg ccatgaacac cggccacgac
      961 gggtcgctga ccacggtgca cgccaacagc ccccgagaca ccctggcccg cctggagacg
     1021 ctggtcctca tggccggctt cgacctgccg gtgcgggcga tccgggagca gatggcctcg
     1081 gccatcgacg tcatcgtcca gctgtcccgc ctccgtgacg gcacccgccg ggtcacccac
     1141 atcaccgagg tgcagggcat ggagggcgac gtgatcaccc tccaggacgt gttcctgttc
     1201 gacttcggtg ccggcgtcga cgagaacggc cgctaccgcg gtcagctcca ggccaccggg
     1261 gtcaggccca agttcgccga gaagctcgcc gaccagggca tccgcctggg cccggaggtc
     1321 ttcgcccgcg acggcggtcg caaggcggtg accggccggt gagcaccatg cgcaacaccc
     1381 ggtcgcgact cggggcgctg atggtcggac tcgcggccgc ggcgtgcctg ttcggcctcg
     1441 tcggagtcgt cgggtccgcc ggagcggccg aggatccgcc gccgtcgttg accgtgcaga
     1501 aggtcgacgc ccgcggcgac gaggtcaacg ccttcggcaa cctggtgggc ggcgacgccc
     1561 agcggctgcg ggccaaggtc aacggcaagg acgtggccgc ggacgtggtg gcgggctccg
     1621 acctgccgct cgacgccgtc gtcgtgctcg acaattcggc ctcgctcggc aacgcgacgg
     1681 tccagttggc caagcagtcg ctcgccccgc tgctaccggg caaggggatc acccgctccc
     1741 tggcgctggt gaccaccggt ggaggagcgt cggtggccat cggcagcacc acctcggccg
     1801 aggccttcca ggcagccctc gacgatgtcg agccggtcgg agcgtcgctc acctgggacg
     1861 ggctcgtccg cgccgcggac atcatcggga acgacggatc ggcccagcag aacatcatcc
     1921 tgttctcggc gtcgccccca caggtcggca ccgcgaccgc ggcaagcgcc caaggggcca
     1981 tggcccaggc cggcgcccag ctcaacgccg tggtcatccc ccgcggcgcc gatctcgagg
     2041 cgatcgacaa catggtcgcc ggcctcggcg ggtcggtgac gatggcggcc gacgaggagg
     2101 gcctcgacga ggccgtcgac tccgtcgcgg tgcagctgca gggtcgcttc gtcctgcgct
     2161 acccgtcgac gggatcgtcg tcgagcacca ccctgaccgt gtcggccggg aagctgtcga
     2221 ccaccgtcgc ctacgtcccg ggtacgacca agagcggagc ggccaacctg gccccgccgg
     2281 tctcggtgag cccgtcgatc accgaacgca tcctcggcaa cccgctgggc ctcatcctga
     2341 tcgtgctgat cggcttcggt gcggtcgccc tgttcatctg gaccctgttg aacatggtcc
     2401 tgccgagctc ggacaacctc aaccgtcgcc tcaaggtcta cgaggacccg tacggcgaat
     2461 cgggggagga ggccgacgaa ccggccgacg gctcccacac gacggtgccg atcatccagc
     2521 gagcggtcga gttcaccggc gacgtcgccc agaagcgggg tctgctcgag aagctcgaag
     2581 gcgacctgga gcgcgccaac ctgccgctgc ggggcgccga ggcgctgttc ttcctcgccg
     2641 tcgcagtggt gctgatgtcg ctgttgacct tcgccctcac ccggagcctg ctgcccgccc
     2701 tcgtcgtggc cgccgccacc gtgctcatcc cccgggcggt gctgagcatc gccgtgcggc
     2761 gacgctgccg ggcgttcgaa cagcagctgc ccgacacgct caccctcatg gccggcaccc
     2821 tgcgggccgg ctactcgatc ggacagggct tcgaggcggt ctcgaccgag atccccgatc
     2881 cgatgggccg tgagctgcgt cgggtcgtca ccgagacccg actgggccgc ccgctcgacg
     2941 aggcgctcga gtcggtcgcc gagcgcatga agagcgacga cttcagctgg gccgtcatgg
     3001 ccatccgcat ccagcgcgag gtcggcggca acctggccga gctcctggtc accgtggccg
     3061 agacgatgac ccagcgtgag cgactccgcc gggacgtcgc caccctcacc gccgaaggcc
     3121 ggatgagcgc catcgtgctg ggcttcctgc caccggcgct cggtctggtc atgtgggcga
     3181 tcaaccccgc ctacatgcag aagctcatga cgcccggact gggctacatc ctgttgggtc
     3241 tgggtctggt ctcgatgctc atcgggttcg cctggatgaa gaagatcatc acgatcgagg
     3301 tgtgacgaga tgtcgccgat cctgctcctg tccgtcctcc tgatcgccgg cgccgtcggt
     3361 gtcgccgtgt gggcggtcct cagccagctc gacgagcgtc aggtcgtgcg cgaggccctg
     3421 cgccagctcg acggctacga ggtcgagaac cagcgcgaca agcagatgct ggccccgctc
     3481 aaggaccgtg ccatcggccc gctcgtcgag ggggtcaccg gtctgggccg acgcttcacc
     3541 ccggtgggct acgtcgagaa ggtcaagcag aagctggtct acgccggcaa ggccgatcag
     3601 gccgcggtcg accggttcct gggcatccgg gtggtcacca tcggcgtcgc cgtgctggtg
     3661 ttcgtcctgc tgttcatgtt caacatcctc gggctgagcg gcacgctgag actggcggtg
     3721 cccgggttgt tcatcctcgc cctggttctc ggacccgaca gcatgctcaa ccgtcaggtc
     3781 gaggagcgcc agcacgagat ccagatcacg cttcccgacg tgatggacct gctgacgatc
     3841 tcggtcgagg cgggcctcgg cttcgagcag gcgatcgacc gggtggtcag ctcggtgccc
     3901 ggtgcgctgt ccgacgagtt ctcccggatg ctgggcgaga cccgtgccgg agcgtcgcga
     3961 tcggacgcca tgcgggccat ggacgatcgc tgcaacgtgc cggagctccg atcgttcgtg
     4021 atggcgatca tccaggccga ccagttcggc gtgtcgatcg gccgcgtgct gcgcggccag
     4081 gccgacgaga tgcgcatcaa gcggcgtcag ctcgcacagg agcgggcgca gaaggcgccg
     4141 gtcaagatgc tcatcccgat ggtgttctgc atcttcccgg ctctgttcgt cgtggtgctc
     4201 ggcccggcga tcatgaacat ccgcgagagc ctgtgaccgc gacccgcccc gcgggcgggg
     4261 cggatggcgc gctggatgag ggcgcggtgt tcggggacgc ggtattcgag ggcgcggtgc
     4321 tcgagggtgc ggtggccgcg gacgcgccgg agcaggacaa cccggatgcg gacgagtcca
     4381 ccggctctgc gccgaggcga cggtggcccg acggcgggct cggcgtgctg gtggccgggc
     4441 tgctggcgtt cgtggccgcc cggcccctga ccgacaacag cttcctgacc cacctggcga
     4501 ccggccggct gatcctggaa caggg
//
LOCUS       DAOQOD010000223         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8448_length_4490_cov_11.062007, whole genome shotgun sequence.
ACCESSION   DAOQOD010000223 DAOQOD010000000
VERSION     DAOQOD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8448_length_4490_cov_11.062007"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2256)
                     /gene="fusA"
                     /locus_tag="PLZ72_14780"
     CDS             complement(160..2256)
                     /gene="fusA"
                     /locus_tag="PLZ72_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNA37611.1"
                     /translation="MAQFPLDRTRNIGIMAHIDAGKTTTTERILFYTGKTYKIGEVHE
                     GGATMDWMEQEQERGITITSAATTAFWTNTVNNTEHRIQIIDTPGHVDFTVEVERSLR
                     VLDGAVAVFDGVAGVEPQTETVWRQANKYNVPRMCFVNKMDRMGADFYGALASIQDRL
                     EAKTAVIQLPIGAEADYKGIVDLVTMKAWIWAGEDLGASWDVVDIPADMQDKADEYRE
                     MLIDVLSETNDEIMEKFLEGDELSPELIRAALRDATINHGVIPVLNGTAFKNKGVQPL
                     LDAVCFYMPSPLDLPPVTGENRKGEEITRKPDVNEPFSALAFKIMTAPHVGKLTYFRV
                     YSGKLEKGGTVYNSRSGDKERIGRLLEMHANDQIEQEAVFAGDIVAGIGIKNTRTGDT
                     LSDPSDPIVLESLEFPDPVIHVAVEPKTKADQDKMGKALFALSEEDPTFQVRTDEETA
                     QTIISGMGELHLEVLVDRMMREFKVEANVGKPQVAYRETITQPVEGYTYLHKKQTGGT
                     GQYAKIVLTLTPSGPGGGFEFTDKITGGRVPKEYIPSVGHGVADAMTNGILAGFPMVD
                     IHVSLDDGDYHDVDSSEMAFKIAGTMGFKEAARKAAPVLLEPIMAVEVVTPEDYMGDV
                     IGDLNSRRGKVGKMEQRGNNQIVSAEVPLSDMFGYSTDLRSKTQGRAQYTMQFHSYQQ
                     TPRNVQEEIVARITGQ"
     gene            complement(2337..2807)
                     /gene="rpsG"
                     /locus_tag="PLZ72_14785"
     CDS             complement(2337..2807)
                     /gene="rpsG"
                     /locus_tag="PLZ72_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNA37612.1"
                     /translation="MPRKGPAPRRQLQPDPIYRSVLVTQIVNKVLQRGKRSTAERIVY
                     DALATIEEKTGTDPVVTLKRAIDNVKPQLEVKSRRVGGATYQVPVEVKPNRSNTLAIR
                     WVVGYARQRRERTMAERLANELLDASNGVGAAAKRKEDLHKMAESNRAFAHYRF"
     gene            complement(2809..3180)
                     /gene="rpsL"
                     /locus_tag="PLZ72_14790"
     CDS             complement(2809..3180)
                     /gene="rpsL"
                     /locus_tag="PLZ72_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNA37613.1"
                     /translation="MPTIEQLVRKGRQTKRRKESTPALKGAPQRRGVCTRVFTSTPKK
                     PNSALRKVARVRLSSGIEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGTL
                     DTSGVRDRKQARSRYGAKKES"
     gene            3651..>4490
                     /locus_tag="PLZ72_14795"
     CDS             3651..>4490
                     /locus_tag="PLZ72_14795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNA37614.1"
                     /translation="MATAPDSVTYLSTAASLRAGDGFGNWLESPMATFPPGWPGVIAG
                     VPFVSTSTAAFLVLALSLLLLPLLSLGILRRTTSSTPARIIGVVAVTLSPTVLPWTYT
                     GLSEVPFTALVLGVVLAALASRESRRWLWVAAGLAALAPLVRYIGVSTPIALAAWVAF
                     DRRRARRWITAAGVVVIGLVPIGGLLLWNQSQAGASMGGRTASALSPIQAAGQALATI
                     GRTAVWTFDRVPTPIGLLLSVAVIALAALAVFGARASDVERARRGRALLALIAVVQTL
                     TLIA"
BASE COUNT          595 a         1408 c         1632 g          855 t
ORIGIN      
        1 attcacgtga ggcttcgtcc gctcgaactt ctccttcgcc atgacaggca ctctccttca
       61 ttcagcccgg acagacaggg cgacttcgtt ggtgggttgg aattgctggt gtgacggtct
      121 ctggtgcgaa ccgacgggac gaccgccggt tcgcgtcgat cactgcccgg tgatgcgggc
      181 gacgatctct tcctgcacgt tccgcggggt ctgctggtag ctgtggaact gcatcgtgta
      241 ctgggcccgg ccctgggtct tggaccgcag gtcggtcgag tagccgaaca tgtccgacag
      301 cgggacctcg gccgagacga tctggttgtt gccccgctgc tccatcttgc cgaccttgcc
      361 gcgacgggag ttgaggtcgc cgatcacgtc acccatgtag tcctcggggg tgacgacctc
      421 gacggccatg atcggctcga ggagcaccgg cgcggctttg cgggcggctt ccttgaagcc
      481 catggtcccc gcgatcttga acgccatctc cgaggagtcg acgtcgtggt agtcgccgtc
      541 gtcgaggctg acgtggatgt cgaccatggg gaacccggcc aggatgccgt tggtcatggc
      601 gtcggcgacg ccgtggccga ccgacgggat gtactccttg ggcacgcggc cgccggtgat
      661 cttgtcggtg aactcgaatc cgccgccggg acccgacggg gtgagggtca gcacgatctt
      721 ggcgtactgg ccggtgccgc cggtctgctt cttgtgcagg taggtgtagc cctcgacggg
      781 ctgggtgatg gtctcgcggt aggcgacctg gggcttgccg acgttggcct cgaccttgaa
      841 ctcgcgcatc atgcggtcga cgagcacctc gaggtgcagc tcgcccatgc ccgagatgat
      901 ggtctgcgcg gtctcttcgt cggtgcggac ctggaacgtc gggtcctctt cggagagggc
      961 gaacagcgcc ttgcccatct tgtcctggtc ggccttggtc ttgggctcga ccgcgacgtg
     1021 gatcaccggg tcggggaact cgagggactc gagcacgatc gggtccgacg ggtccgacag
     1081 ggtgtcgccg gtgcgggtgt tcttgatgcc gatgccggcg acgatgtcac cggcgaacac
     1141 ggcttcctgc tcgatctggt cgttggcgtg catctccagc agacgaccga tgcgctcctt
     1201 gtcaccggag cgcgagttgt agaccgtgcc gcccttctcg agcttgccgg agtagacgcg
     1261 gaagtaggtg agcttgccga cgtggggggc ggtcatgatc ttgaacgcca gggccgagaa
     1321 cggctcgttc acgtcgggct tgcgggtgat ctcctcgccc ttgcggttct cgccggtgac
     1381 cggcggcagg tcgagcggcg acggcatgta gaagcacacc gcgtcgagca gcggctgcac
     1441 gcccttgttc ttgaaggcgg tgccgttgag gaccgggatc acaccgtggt tgatggtggc
     1501 gtcgcgcagc gcggcgcgga tcagctccgg ggagagctcg tcgccctcga ggaacttctc
     1561 catgatctcg tcgttggtct ccgacaggac gtcgatgagc atctcgcggt actcgtcggc
     1621 cttgtcctgc atgtcggcgg ggatgtcgac gacgtcccag gacgcgccca ggtcctcgcc
     1681 ggcccagatc caggccttca tggtgaccag gtcgacgatg cccttgtagt cggcttcggc
     1741 gccgatcggc agctggatga cggcggtctt ggcctcgagg cggtcctgga tggacgccag
     1801 cgcaccgtag aagtcggcgc ccatgcgatc catcttgttg acgaagcaca tgcgggggac
     1861 gttgtacttg ttggcctgac gccacaccgt ctcggtctgg ggctccacgc cggcgacgcc
     1921 gtcgaacacc gcgacggcgc cgtcgaggac gcggagcgaa cgctcgacct cgacggtgaa
     1981 gtcgacgtgg ccgggcgtgt cgatgatctg gatgcggtgc tcggtgttgt tgaccgtgtt
     2041 ggtccagaac gccgtggtgg ccgcggaggt gatggtgatc ccccgctcct gctcctgctc
     2101 catccagtcc atggtggcgc cgccctcgtg gacctcgccg atcttgtagg tcttgccggt
     2161 gtagaagagg atccgctcgg tcgtcgtcgt cttgcccgcg tcgatgtggg ccatgatgcc
     2221 gatgttgcgg gtgcggtcga gggggaactg cgccatcgtc gtcgtctcag tcttggtgtg
     2281 cgggggtgtt cggggtggag gtcggagtcg accggcgcag gatcagcgcc gcggactcag
     2341 aagcggtagt gggcgaaggc ccggttggac tcggccatct tgtgcaggtc ttccttgcgc
     2401 ttggccgcgg cgccgacgcc gttgctggca tcgagcagct cgttggcgag gcgctcggcc
     2461 atggtgcgct cgcgacgctg acgggcgtag ccgacaaccc agcggatggc gagggtgttg
     2521 gagcggttgg gcttgacctc gacgggcacc tggtaggtgg cgccgccgac gcgacggctc
     2581 ttgacctcga gctggggctt gacgttgtcg atggcccgct tgagcgtgac caccgggtcg
     2641 gtcccggtct tctcctcgat ggtggcgagg gcgtcgtaga cgatgcgctc ggcggtggag
     2701 cgcttgcccc gctgcaggac cttgttgacg atctgggtca cgagcaccga ccggtagatg
     2761 gggtcgggct ggagctggcg gcggggggcg ggacccttgc ggggcatgtc aggactcctt
     2821 cttggcgccg tagcgcgaac gtgcctgctt gcggtcgcgg accccggagg tgtcgagcgt
     2881 gccgcggatg atcttgtagc ggacgccggg gaggtccttc acacgaccgc cgcggacgag
     2941 caccatcgag tgctcctgga ggttgtgtcc ctcaccgggg atgtaggccg tgacctcgat
     3001 gccgctggag aggcggacac gagcgacctt gcgcagagcc gagttgggct tcttgggcgt
     3061 cgaggtgaac acgcgagtgc agaccccacg ccgctggggc gcgcccttca gcgccggcgt
     3121 ggactccttg cggcgcttgg tctgacggcc cttgcggacc agctgctcga tcgtaggcac
     3181 ggtgaacctc tgcgttgtcg tgatctcagg tctcgacgac ctcaccgtgg tggcggacgg
     3241 caccggtgaa ccggcacgtg ccgaccggcg gcaatcgctc gcacgcacgg aggtgacgtc
     3301 gacgccgatg gtcgtggcgc gacgcaaacg cagccatctc cttgggcgac cgagaaagac
     3361 tacggcctca cctgccccaa ggcaaaccac cctgacgacc gactcggtcg gcccggcgcg
     3421 ggtcaccctc tggtcaccga tgtccggatg tgacagcctg tcggcgggga ggtagagggc
     3481 gcatgaccga tcaggcgggt gtttcggtcc cggtcacgaa ggttgccgac acggatggcg
     3541 atcagggtgg cgacgggacc cggacgaccg gggcgagggc ggagcagatc gtcgcatggg
     3601 cggcaggcct gctggcggtg gcgatctcgt ggtacgcgag tcgtggtcgc atggccaccg
     3661 ctcccgacag cgtcacgtac ctgagcaccg ccgcgtcgct gcgggccggt gacgggttcg
     3721 ggaactggct cgagtcaccc atggccacgt tcccgcccgg atggcctggt gtgatcgccg
     3781 gcgtcccctt cgtgagcacg tcgacggcgg cgttcctggt gctcgcgctg tcgctgctgc
     3841 tgctcccgtt gctgagcctc gggatattgc gtcggacgac ctcgtcgacg ccggcacgga
     3901 tcatcggcgt cgtcgccgtg accctctccc cgacggtcct cccttggacc tataccggac
     3961 tgagcgaggt gccgttcacg gcgctggtgc tcggcgtcgt gctcgccgct ctcgcgtcga
     4021 gggagtcccg tcgttggctg tgggtcgcag cgggtctcgc tgcgctcgcg cccctcgtcc
     4081 gctacatcgg cgtctccaca ccgatcgcgc tcgccgcgtg ggtggcgttc gaccgtcgcc
     4141 gcgcccggcg gtggatcacg gccgccggcg tcgtggtcat cggactcgtg ccgatcgggg
     4201 gtctgctcct ctggaaccag tcgcaggccg gtgcatccat gggagggcgg acggcatcgg
     4261 ccctgtcgcc gatccaggcg gcgggccagg cactggcaac gatcggacgc actgcggtgt
     4321 ggaccttcga ccgggtgccg acgccgatcg gactgctcct gtccgtggcc gtgatcgcgc
     4381 tcgcggcact cgccgtgttc ggcgcgaggg catcggacgt cgaacgcgct cggcgcggtc
     4441 gggcgctgct cgcgttgatc gctgtggttc agacgctgac gctcatcgcc
//
LOCUS       DAOQOD010000224         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8527_length_4464_cov_9.217283, whole genome shotgun sequence.
ACCESSION   DAOQOD010000224 DAOQOD010000000
VERSION     DAOQOD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_4464_cov_9.217283"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLZ72_14800"
     CDS             complement(<1..849)
                     /locus_tag="PLZ72_14800"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNA37615.1"
                     /translation="MRALRFERDVVRFGAARLASGLRPGAGSRWGPLELVTDDEPDLP
                     GPDWVRLSPRLAGICGSDLATVDGRSSRWFEPIVSFPFVPGHEVVADLVPDGSTDATA
                     DARAHATADGASSRVVLEPVLGCVTRGISPVCPACADGRLGNCEHLAHGDLDAGLQTG
                     YCCDTGGGWSTSMVAHPSQLHAVPDDMTDQAAVLVEPTACAVHGAMRARVRPGDTVAI
                     IGAGTLGLLTLAALARFAPECSTVIGAKHPHQRDLARALGATVVCEPGELARAVRRRT
                     GTQVLGT"
     gene            complement(846..>2129)
                     /locus_tag="PLZ72_14805"
     CDS             complement(846..>2129)
                     /locus_tag="PLZ72_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNA37616.1"
                     /translation="TGDANPLRYQQLVDRVRDWFTERPLYDRNGQPIVVPDWTFPGRG
                     RVEGQLRRATSVLERAERVVAALPLRGTAAQWGAGIEEKREQARRALSYVELYGAYTE
                     CEAVYGVQRLAELDSRLSAEDHELFTLDPRVIDWDRYITEVHLPSVVAHGRARSSPSK
                     RSATARTDRLRAQVLAPERQMAVFDLENTIIASNVVATYSWLATRRLGTADRVRFVGR
                     TLLEAPALLAQDRRDRSDFLRAFYRRYEGAPVEELRQDAAEHFSDLLLTRSFPDAVRR
                     VRRHRELGHTTVLITGALDIAIEPLTPLFDHVVCARLGTRVESTRSGPTEVLTGHLEE
                     VPPTAEARARIIVDLCSELDLDPRQAVAYADSSSDLPMLEAVGFPVAVNPEPRLASIA
                     RKRGWLVEDFGSDFGHKVLPMGPAWRERRTGRRLA"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2230..3275)
                     /locus_tag="PLZ72_14810"
     CDS             complement(<2230..3275)
                     /locus_tag="PLZ72_14810"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37617.1"
                     /translation="MIVDGLAGTRIFVTGATGFVGTALVERLLRCVPDVQLTLLIRDG
                     RRTTAQRRLEREVLRNDCFDRLRSELGANGFAEVAARVRAVSGDVGSDGLGLDDDGRA
                     ALSETDVVIHSAATVAFDSPLDRAVEINLMGPVRIAETLARLGASPHLVAVSTCYVAG
                     NRRGAAPEEPVTANPFFADLDWRAEVDAARRTRADVDAESRTPERLDEFRARARSELG
                     PAGGPLIAERTESLRKDWVHDRMVTFGRSRATSVGWPDAYAFTKALGEVALTRTLDRV
                     RSEGGPDIGCTIVRPSIIESALAEPRPGWIRGFRMAEPIILSYARGLLREFPGVPEGV
                     IDVIPVDLVVAAIIA"
     gene            complement(3367..>4464)
                     /locus_tag="PLZ72_14815"
     CDS             complement(3367..>4464)
                     /locus_tag="PLZ72_14815"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNA37618.1"
                     /translation="PERIRLVVLFGGRSAEHDVSRVSASHVMSAIDPDRYEVVPVGIT
                     RDGDWQLAEQALALLADRRSLPPSIDVAGPRADPLALTTADTDARRTVVIPVLHGPNG
                     EDGTVQGLLELAGLPYVGAGVLGSAVAMDKVMAKTVLDAHGIPQPRWRAVPVNDLSAP
                     GLAEDLLAELGPVVFVKPANMGSSIGVSRADDVDSVLEALATAARYDELLVVEEGIGS
                     PTERVRELECGVLGNLSPRASVVGEIVPAADFYDYEDKYADGKALTVIPAEIDDETSR
                     TVRELAVRTFRALRAEGMARVDMFLTPASDPAGSRLLVNEINTLPGFTPISMFPMLWR
                     ASGLEYPQLIDELVRLALERHDRRSRFETGR"
BASE COUNT          606 a         1546 c         1659 g          553 t
ORIGIN      
        1 cgtgccgagg acctgtgtgc cggtgcggcg ccggacggcg cgggccagct caccgggctc
       61 gcagaccacg gtcgcgccga gcgcccgggc caggtcgcgt tggtgcgggt gcttggcgcc
      121 gatcacggtg ctgcactcgg gcgcgaagcg cgccagcgcg gccagggtga gcagtccgag
      181 ggtgccggcc ccgatgatcg cgacggtgtc gccggggcgg acccgtgccc gcatcgctcc
      241 gtggaccgcg cacgcggtgg gctcgaccag caccgcggcc tggtcggtca tgtcgtcggg
      301 caccgcgtgc agctggctgg ggtgcgccac catcgaggtg gaccagcctc caccggtgtc
      361 gcagcagtag ccggtctgca gtcctgcgtc gaggtcgccg tgggccaggt gctcgcagtt
      421 gccgagccgg ccgtcggcgc acgccgggca caccggcgag atcccccggg tgacgcagcc
      481 gagcacaggt tcgagcacca cccgggagga tgcgccgtcg gccgtggcgt gggccctggc
      541 gtcggccgtg gcgtcggtcg acccgtcggg aaccaggtcg gcgacgacct cgtggccggg
      601 cacgaacggg aacgacacga tcggctcgaa ccacctcgac gagcggccgt cgacggtggc
      661 caggtccgag ccgcagatcc ccgccagccg aggtgacagg cgcacccagt ccgggccggg
      721 gaggtcgggc tcgtcgtcgg tgaccagctc cagtggcccc cacctcgacc ccgccccggg
      781 ccgcagaccc gaggcgagcc gcgccgcgcc gaagcggacg acgtcacgct cgaagcgcag
      841 cgccctcacg cgagcctccg gccggtgcgt cgctcccgcc aggccggacc catcgggagc
      901 accttgtggc cgaagtccga cccgaagtcc tcgaccagcc atccccgctt gcgggcgatc
      961 gacgccaacc ggggttcggg gttgaccgcg accgggaacc cgacggcctc gagcatgggc
     1021 aggtccgacg acgagtccgc gtacgcgacc gcctggcggg gatccaggtc gagctccgag
     1081 cacaggtcga cgatgatgcg ggcccgggcc tcggcggtgg gggggacctc ctcgaggtgg
     1141 ccggtcagca cctcggtcgg cccggagcgg gtcgactcga cccgggtgcc gaggcgggcg
     1201 cagaccacgt ggtcgaacag cggtgtgagc ggctcgatgg cgatgtcgag cgcgccggtg
     1261 atcagcaccg tggtgtggcc gagctcgcgg tggcggcgca cccgccgcac cgcgtcgggg
     1321 aacgagcggg tcagcagcag gtcgctgaag tgctcggccg cgtcctgtcg cagctcctcg
     1381 accggagcgc cttcgtagcg gcggtagaac gcccgcagga agtcggatcg gtcccggcgg
     1441 tcctgggcga gcagggccgg tgcctcgagc agggtgcgtc cgacgaagcg gacccggtcc
     1501 gcggtcccga ggcgccgggt ggccagccag gagtaggtgg cgaccacgtt ggaggcgatg
     1561 atcgtgttct cgaggtcgaa caccgccatc tggcgctccg gcgccagcac ctgggcccgc
     1621 aggcggtcgg tgcgggcggt ggcggagcgc ttggacggcg acgagcgggc ccgtccgtgg
     1681 gcgacgaccg agggcaggtg gacctcggtg atgtagcggt cccagtcgat cacccgcggg
     1741 tcgagggtga acagctcgtg gtcctcggcc gacaggcggg agtcgagctc ggccagccgc
     1801 tgcaccccgt agaccgcctc gcactcggtg taggcgccgt acagctcgac gtaggacaac
     1861 gcccggcggg cctgctcgcg tttctcctcg atcccggctc cccactgcgc cgcggtgccc
     1921 cgcagcggca gtgcggcgac gacccgttcg gcgcgttcga gcaccgaggt ggcccgtcgc
     1981 agctggccct cgacccgacc gcgaccgggg aaggtccagt cgggcaccac gatcggttgg
     2041 ccgttgcggt cgtacagcgg tcgttcggtg aaccagtccc gcacccggtc gaccagctgc
     2101 tggtaccgca gcgggttggc gtcgccggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnng cgatgatggc ggcgacgacc aggtcgacgg ggatgacgtc gatcacgcct
     2281 tcgggcaccc cggggaactc tcgcagcagg ccccgggcgt agctgaggat gatcggctcg
     2341 gccatgcgga agccgcggat ccagccgggg cggggctcgg ccagcgcgga ctcgatgatc
     2401 gacgggcgca cgatcgtgca gccgatgtca gggccgccct cggagcggac ccggtcgagg
     2461 gtgcgggtga gggcgacctc gcccagcgcc ttggtgaacg cgtaggcgtc gggccagccg
     2521 accgaggtgg cccgggaccg accgaaggtg accatgcggt cgtgcaccca gtccttgcgc
     2581 aggctctcgg tgcgctcggc gatcaacggc ccgccggccg ggccgagctc ggagcgggca
     2641 cgcgcccgga actcgtccag gcgctcagga gtgcggctct cggcatccac atcggcccgg
     2701 gtgcgtcgcg ccgcgtcgac ctcggcccgc cagtccagat ccgcgaagaa cgggttggcg
     2761 gtgaccggct cctccggagc cgcaccgcgg cggttgccgg ccacgtagca cgtgctgacc
     2821 gcgaccaggt gaggtgacgc cccgagccgg gccaacgtct cggcgatgcg caccgggccc
     2881 atcaggttga tctcgaccgc gcggtcgagc ggcgagtcga acgccacggt cgcggccgag
     2941 tggatcacca cgtcggtctc ggacagcgcg gcccgtccgt cgtcgtcgag gccgagcccg
     3001 tcggagccga cgtcgccgct gaccgcccgg acccgcgccg ccacctcggc gaagccgttg
     3061 gctccgagct cgctgcgcag ccggtcgaag cagtcgttgc gcagcacctc gcgctccagg
     3121 cgccgctggg cggtggtgcg gcgaccgtcg cggatcagca acgtgagctg cacgtcgggc
     3181 acgcagcgca gcagtcgctc gaccagcgcg gtgccgacga agccggtcgc gccggtgacg
     3241 aagatccgcg tgccggccag accgtcgacg atcacggccg cttcactagc agacaggggg
     3301 ctgcgctggc agacccgggg cgcagggaca gggcgtcggc acggaggccg gagggtgccg
     3361 ccatgctcat cgtccggtct cgaagcggga gcggcggtcg tggcgctcca gcgccaggcg
     3421 gaccagctcg tcgatcagct gcgggtactc cagtcccgac gcccgccaca gcatcgggaa
     3481 catcgagatc ggcgtgaagc cgggcagcgt gttgatctcg ttgaccagca ggcgagaacc
     3541 cgccgggtcc gaggccggcg tcaggaacat gtcgacgcgg gccatgccct cggcccgcag
     3601 cgcccggaag gtgcgcaccg ccagctcccg gaccgtccgc gaggtctcgt cgtcgatctc
     3661 ggcggggatg acggtgaggg ccttgccgtc ggcgtacttg tcctcgtagt cgtagaagtc
     3721 cgcggcgggg acgatctcgc cgaccaccga cgcccgcggc gacaggttgc ccagcacccc
     3781 gcactcgagc tcgcggaccc gttcggtggg cgagccgatg ccctcctcga cgaccagcag
     3841 ctcgtcgtat cgcgccgcgg tggcgagcgc ttcgagcacg gagtcgacgt cgtcggcgcg
     3901 cgacacgccg atcgacgagc ccatgttcgc cggcttgacg aacaccaccg gtcccagctc
     3961 ggcgagcagg tcctcggcca gacccggggc gctcaggtcg ttcacgggga ccgcccgcca
     4021 gcggggctgg gggatcccgt gggcgtcgag gacggtcttg gccatcacct tgtccatcgc
     4081 caccgcggag ccgaggacgc ccgcgccgac gtacggcaag ccggcgagct cgagcaggcc
     4141 ctggacggtg ccgtcctcgc cgttcggtcc gtggagcacg gggatgacca cggtgcggcg
     4201 cgcgtcggtg tcggcggtcg tcagggccag cggatcggca cgggggccgg ccacgtcgat
     4261 cgacggcggc agcgaccgac ggtccgccag cagcgccaga gcttgctctg ccagctgcca
     4321 gtcgccgtcg cgggtgatgc cgacgggcac gacctcgtag cggtcggggt cgatggccga
     4381 catcacgtgg ctggccgaca cccggctgac gtcgtgctcg gcggatcgcc caccgaacag
     4441 cacgaccagg cggatgcgct ccgg
//
LOCUS       DAOQOD010000225         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8536_length_4462_cov_12.670524, whole genome shotgun sequence.
ACCESSION   DAOQOD010000225 DAOQOD010000000
VERSION     DAOQOD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_4462_cov_12.670524"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1694
                     /locus_tag="PLZ72_14820"
     CDS             72..1694
                     /locus_tag="PLZ72_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37619.1"
                     /translation="MTSRTYDAVVIGAGHNGLCLAAYLQRAGLSTAIIERRHEEGGGV
                     NTEEPVLSGYRHNMHAQFMEFFDVMPMIQDFGLEDLGLRTVMPEAQAGIAFADGRPPV
                     ILHRPDLLDRTHASIARYSRSDADTFVELKQRAARFEELLAVGLYNPPGSTAAVGMGT
                     LEGRAAILEGTFGDLGVTGHFALKTPKMVIDEMFETPELRALMYRVSVEWGLPIDTAV
                     TGTDFLTFLMWTTANWKLVMGGTHGLAKAMTQACYREGVDLIENAHVDRILVEDGRAV
                     GVVARGVEYRAEKLVASNADVHQTLIGLVGEEHLSPLWAKRAKDFRFGPSHVLATPMF
                     CLYEAPAYRSARWDPEIDKCFYTVVGYDGPDDMARYIRDAYSGLLPAPAAGTWVNSLW
                     DRSQAPPGRHAATGWYFFPVASELSTEEWEEVRTTFNDRFLARWREFAPNMTADNVIA
                     HKLYTPDQMERKNMMREGDFSHGEFVPDQQGVNRPFPEASNYRTEIDGLYLCGSSAYP
                     GGGVHAACGYNAYKKIAEDFGLPSPIGSGRSY"
     gene            1694..2161
                     /locus_tag="PLZ72_14825"
     CDS             1694..2161
                     /locus_tag="PLZ72_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37620.1"
                     /translation="MPDIYTPEWYDAVKDAINAAVDGLSDTPEGAWTICVEIVGDGAS
                     PYTPADGMRRFLIRIEQGRCLWYRELDADPDEKLDYRFTGDASVFDGIAAALDDPIEI
                     VLHGSIKVRGDMRFLLRQAELVNVLLGAYTSGVDTTWPLGRPPYAAVAGAVAP"
     gene            2158..3753
                     /locus_tag="PLZ72_14830"
     CDS             2158..3753
                     /locus_tag="PLZ72_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37621.1"
                     /translation="MTRTYDAVIIGAGHNGLTLGAYLARSGLDVVVLERRHEEGGGLC
                     TEEVTRPGFLHNIHANYHTFVGMAPPVTDLDVRGHGIEYVRPEVQMASVFDDDTALCI
                     YEDIDRTVEAMAEFSAQDAATFKRLYDEAHGFVDLLLGTLMYQPPLSVKELTKALSTF
                     GVDKRSEFLSVKLRRETINQFLDAHFTNPKIKAHLAFHGAVCSYTNDVMGLAIGFPLL
                     VGKINNWHLCIGGSHRLAHALWRDLAQHGGVMISDAEVDRILVDGGRAVGVRLADGTE
                     IEARKLVASTVSAEQTFLEFLDSSVVGEELTHKVKTEIQHKDWSLFSVHLAMAALPQY
                     RAASFNPDVDRAWVVNLGIDSTGRFDEDWARIRAGELVDPHPNAAVNSLYDPTDAPEG
                     FYTGLLRQFAPFALADGGEGTWDGIGRSYGHRCIEAWRRYAPNLTDDALVEWDVFTPL
                     DIARKLPNMVKGDWMMGEISLANMLDQRPLPELGQYRTPVDGLYMAGSTQHPHGFITF
                     GPGYNALQVIADDLDIDKWWAQV"
     gene            3750..4364
                     /locus_tag="PLZ72_14835"
     CDS             3750..4364
                     /locus_tag="PLZ72_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37622.1"
                     /translation="MSASSVEAVGSVPPVVLRDGDDASGMAVMIAGLLEDNLRDFPGR
                     ARVAARTRGSVVLTASDRDVSITVTFGSDGVEIADGARPGVAAMSGPWLTMSRVCSGR
                     ITPWRAIRDRELALGWGRSVWALPAAGFVLSVPASFYEDASVGSDADGAGAGDGRGSA
                     ADHRVVAGAVVAGATVAVVGGSVLIARASRRRRRRRSSSPHPNL"
BASE COUNT          674 a         1570 c         1537 g          681 t
ORIGIN      
        1 gcgatccgtt gacccggcgc gcgactccga acatattgtc gagcaaccac tagaagtatc
       61 gggggtcagc ggtgacgagt cgcacgtacg acgccgtggt gatcggggcg ggccacaacg
      121 gtctctgcct cgccgcctac ctccaacggg ccgggctctc gacggcgatc atcgaacgtc
      181 gccacgaaga gggcggaggc gtcaacaccg aggaaccggt gctgtcgggc taccgccaca
      241 acatgcacgc ccagttcatg gagttcttcg acgtcatgcc gatgatccag gacttcgggc
      301 tcgaggacct gggtctgcgc actgtcatgc ccgaggccca ggccggcatc gccttcgcgg
      361 acggccggcc gccggtcatc ctgcaccggc cggacctgct ggatcggacc cacgccagca
      421 tcgcccgcta ctcgcgctcc gacgccgaca ccttcgtcga gctcaagcag cgcgccgctc
      481 gcttcgagga gctgctcgcg gtaggcctct acaacccgcc ggggtccacc gccgcggtcg
      541 gcatgggaac cctggagggc cgcgccgcca tcctcgaggg cacgttcggc gacctgggcg
      601 tcaccggcca cttcgcgctc aagaccccca agatggtcat cgacgagatg ttcgagacgc
      661 cggagctgcg ggcgttgatg taccgggtca gcgtcgagtg ggggctgccg atcgacaccg
      721 cggtcaccgg caccgacttc ctgacgttcc tgatgtggac caccgccaac tggaagctcg
      781 tcatgggcgg cacccacggc ctggccaagg cgatgaccca ggcctgctac cgcgagggtg
      841 tcgacctgat cgagaacgcc cacgtcgatc gcatcctggt cgaggacggt cgggccgtcg
      901 gcgtggtcgc ccggggcgtc gagtaccggg ccgagaagct ggtggcgtcg aacgcggacg
      961 tgcaccagac cctgatcggc ctggtcggcg aggagcacct cagcccgttg tgggccaagc
     1021 gggccaagga cttccgcttc ggccccagcc acgtgctcgc cacgccgatg ttctgcctct
     1081 acgaggcgcc cgcctaccgc tcggcgcgct gggaccccga gatcgacaag tgcttctaca
     1141 ccgtcgtcgg ctacgacggc cccgacgaca tggcgcgcta catccgcgac gcctactcgg
     1201 gactgctgcc ggcgcccgcc gcgggcacgt gggtcaacag cctgtgggac cgctcccagg
     1261 ccccgccggg tcgccacgcc gcgaccggct ggtacttctt cccggtcgcc agcgagctga
     1321 gcaccgagga gtgggaggag gtgcgcacca ccttcaacga ccggttcctg gctcggtggc
     1381 gcgagttcgc cccgaacatg accgccgaca acgtgatcgc ccacaagctc tacacgccgg
     1441 accagatgga gcggaagaac atgatgcggg agggcgactt ctcccacggc gagttcgtgc
     1501 ccgaccagca gggcgtcaac cggcccttcc ccgaggcgtc caactaccgg accgagatcg
     1561 acggcctcta cctctgcggg tcctcggcct accccggcgg cggcgtgcac gcggcctgcg
     1621 ggtacaacgc gtacaagaag atcgccgagg acttcgggct ccccagcccg atcggatccg
     1681 ggaggagcta ctgatgcccg acatctacac gccggagtgg tacgacgcgg tcaaggacgc
     1741 gatcaacgcc gcggtcgacg ggctctccga cacccccgag ggggcgtgga cgatctgcgt
     1801 cgagatcgtc ggcgacggcg catcgcccta cacgcccgcc gacgggatgc gccggttcct
     1861 gatccgcatc gagcagggcc gatgcctgtg gtaccgggag ctcgacgccg atcccgacga
     1921 gaagctcgac taccgcttca ccggagatgc cagcgtgttc gacggcatcg ccgccgccct
     1981 cgacgacccg atcgagatcg tcctgcacgg ctcgatcaag gtgcgaggcg acatgcggtt
     2041 cctgctgcgc caggccgagc tggtcaacgt gctgctcggc gcctacacct ccggggtcga
     2101 caccacgtgg ccgctcgggc gaccgcccta cgccgccgtg gccggggcgg tggcgccgtg
     2161 acccgcacct acgacgccgt catcatcggc gccggtcaca acggcctgac cctcggcgcc
     2221 tacctggccc gctccggtct cgacgtcgtc gtgctcgaac ggcgtcacga ggagggcggc
     2281 gggctgtgca ccgaggaggt cacccgcccc ggcttcctgc acaacatcca cgccaactac
     2341 cacacgttcg tgggcatggc gccgccggtc accgacctcg acgtgcgcgg ccacggcatc
     2401 gagtacgtcc gtcccgaggt gcagatggcc tcggtgttcg acgacgacac cgcgctgtgc
     2461 atctacgagg acatcgaccg gaccgtcgag gcgatggccg agttctccgc ccaggacgcg
     2521 gcgacgttca agcggctcta cgacgaggca cacgggttcg tcgacctgct gctcggcacg
     2581 ctgatgtacc agccgccgct gtcggtcaag gagctgacca aggcgctgtc gacgttcggg
     2641 gtggacaagc gctcggagtt cctgtcggtc aagctccgtc gggagacgat caaccagttc
     2701 ctcgacgcgc acttcaccaa ccccaagatc aaggcgcacc tggccttcca cggggcggtc
     2761 tgctcataca ccaacgacgt gatgggcctg gcgatcgggt tcccgctgct ggtcggcaag
     2821 atcaacaact ggcacctgtg catcggtggc tcgcaccggc tcgcccacgc gctgtggcgc
     2881 gacctggccc agcacggcgg ggtgatgatc tccgacgccg aggtcgaccg catcctcgtc
     2941 gacggcgggc gcgcggtcgg cgtgcgcctg gccgacggca ccgagatcga ggcccgcaag
     3001 ctggtcgcct cgacggtgtc ggccgagcag acgttcctcg agttcctcga ctcgtcggtg
     3061 gtcggcgagg agctcaccca caaggtcaag accgagatcc agcacaagga ctggtcgctg
     3121 ttctcggtgc acctggccat ggccgcgctg ccgcagtacc gggccgcctc gttcaacccc
     3181 gacgtcgacc gggcctgggt ggtcaacctg ggcatcgact cgaccggacg cttcgacgag
     3241 gactgggccc ggatccgcgc cggcgagctc gtcgacccgc accccaacgc agcggtcaac
     3301 tcgctgtacg acccgaccga cgcgcccgag ggcttctaca ccgggctgct gcgccagttc
     3361 gccccgttcg ccctggccga cggcggtgag gggacctggg acgggatcgg tcgctcctac
     3421 ggtcatcgct gcatcgaggc gtggcgtcgc tacgcgccga acctgaccga cgacgccctg
     3481 gtcgagtggg acgtgttcac cccgctcgac atcgcccgca agctgcccaa catggtcaag
     3541 ggcgactgga tgatgggcga gatcagcctg gccaacatgc tggaccagcg gccgcttccc
     3601 gagctgggcc agtaccgcac gccggtcgac gggctgtaca tggccggctc cacccagcac
     3661 ccgcacggct tcatcacctt cgggccgggc tacaacgcgc tgcaggtgat cgcggacgac
     3721 ctcgacatcg acaagtggtg ggcccaggtg tgagtgcctc gtcggtggag gccgtcggat
     3781 ccgttccgcc ggtcgtgctg cgcgacggcg acgacgcgtc gggcatggcg gtgatgatcg
     3841 ccgggctgct cgaggacaac ctccgcgact tccccggtcg ggcgcgggtg gcggcgcgga
     3901 cgcggggctc ggtggtgctg acggcgtcgg accgcgacgt gtcgatcacg gtgaccttcg
     3961 gctccgacgg cgtcgagatc gcggacggcg cccggcccgg ggtcgccgcc atgtcgggtc
     4021 cgtggttgac catgagtcgg gtgtgcagcg gtcggatcac cccgtggcgg gcgatccgcg
     4081 accgggagct cgctctgggg tggggtcggt ccgtgtgggc gctaccggcg gcgggcttcg
     4141 tcctgtcggt cccggcgtcg ttctacgagg acgcctccgt cgggagcgac gctgacggcg
     4201 ccggtgctgg cgacggccgc ggctcggcgg cggaccatcg ggtggtcgcg ggggcggtcg
     4261 tggcgggagc aacggtggcg gtggtgggtg gttcggtgct gatcgcccgt gcctcccgcc
     4321 gtcgccgtcg ccgtcgcagc tcgagccccc acccgaactt gtagctgtgc gtcgctgtcg
     4381 tggcgcgtgg gctacaagtt cgttggccgc tggcgatgtt gtaggccacg gtcgctgtcg
     4441 tggcgcgtgg gctacaagtt cg
//
LOCUS       DAOQOD010000226         4439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8605_length_4439_cov_13.786724, whole genome shotgun sequence.
ACCESSION   DAOQOD010000226 DAOQOD010000000
VERSION     DAOQOD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8605_length_4439_cov_13.786724"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /gene="rplA"
                     /locus_tag="PLZ72_14840"
     CDS             complement(<1..515)
                     /gene="rplA"
                     /locus_tag="PLZ72_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNA37623.1"
                     /translation="MAHGKRYLDATKRFDRERHHTADEAIDVVKSLATANFDESVELV
                     VRLGVDPRKADQMIRGTVALPSGTGKDVRIAVFAQGDAATAAEAAGAEFVGAEDLAAQ
                     IEGGMLDFDVTIATPDLMPLVGRLGRVLGPRGLMPNPKTGTVTPDPAKAVEEFKGGKV
                     EYRTDRNGNVHV"
     gene            complement(661..1086)
                     /gene="rplK"
                     /locus_tag="PLZ72_14845"
     CDS             complement(661..1086)
                     /gene="rplK"
                     /locus_tag="PLZ72_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNA37624.1"
                     /translation="MAKKKVVAIVKIQIPAGQASPAPPVGTALGPHGVAIMDFCKEYN
                     ARTEAQRGTIVPVEISIYEDRTFSFVTKTPPAPVLIREAAGLDKAAETPGRGFVGKIT
                     DAQLTEIAQTKLPDLNTDDIDAAKLQIAGTARSMGIEVV"
     gene            complement(1255..2112)
                     /gene="nusG"
                     /locus_tag="PLZ72_14850"
     CDS             complement(1255..2112)
                     /gene="nusG"
                     /locus_tag="PLZ72_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNA37625.1"
                     /translation="MTDQNDIPTTEAADDALQAAVDDEARVEELITEVAGDDAGADAI
                     EALADAEDIDADGVLVQEPVQDDAVLDELVEDEAILDEDDLFDAQEPPAPKPVSPYDR
                     PGKWYVLHTQSGYENKVRQNIEARRASMNMESRIHEVVIPMEDVVEFKQGKKVVVQKK
                     MFPGYLLIRCSLNDDSWYVIRNTPGVTGFVGAANKPQALPRKDVENFLGTGEEVDAEA
                     PATPKRSAGPVHGYEINETVRVKEGPFADFSGEIIEINEDQLKVKVLVNIFGRETPVE
                     LEFAQVAKL"
     gene            complement(2206..2556)
                     /gene="secE"
                     /locus_tag="PLZ72_14855"
     CDS             complement(2206..2556)
                     /gene="secE"
                     /locus_tag="PLZ72_14855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNA37626.1"
                     /translation="MAMNREQKRAMQRAGQLNADGSQAPRDRKAPAQNVKSERTKPLE
                     FVREVRAEMRKVSWPDRQEVIRYSMIVLVALVIFTAGVFVVDYAFGELFRIILDPGNS
                     TSGASGLLTPPTLY"
     gene            complement(2585..2657)
                     /locus_tag="PLZ72_14860"
     tRNA            complement(2585..2657)
                     /locus_tag="PLZ72_14860"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Trp,seq:cca)
     gene            complement(2890..3252)
                     /locus_tag="PLZ72_14865"
     CDS             complement(2890..3252)
                     /locus_tag="PLZ72_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37627.1"
                     /translation="MRCDEVAPLLPAMSDGTVVLPSDMAAHVEHCLRCQAELVQHRRV
                     LRTLRSLRAEVLEPAPGMLTDVLAAVEEVGERHAVRSLLRRRRVAYGAGVAAATVAGV
                     IGAVVINSRSRRSPSLAG"
     gene            complement(3242..3958)
                     /locus_tag="PLZ72_14870"
     CDS             complement(3242..3958)
                     /locus_tag="PLZ72_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNA37628.1"
                     /translation="MSDPHATLLLGDAGPPASPAPPESPAPDLRLLGSAPRPSSGRSE
                     QDPASDDAQAADVDAARSGDHAAFARLVEATHRDTYALALHLTGCVEDARDVTQEAYL
                     RAYRGLGSFRGDARFSTWMYRITANVAATQAGRNRRHRHDELADDSPIADPRPDHDPA
                     LAADAGDLRDRILVALGQLPPKLRAVIVLRDVYELPHDAIAEELGISVTAAKVRLHRA
                     RHRLRSDVFPELAEVPGRAL"
     gene            complement(4074..4235)
                     /gene="rpmG"
                     /locus_tag="PLZ72_14875"
     CDS             complement(4074..4235)
                     /gene="rpmG"
                     /locus_tag="PLZ72_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNA37629.1"
                     /translation="MASDKRVAVTLECTECKRRNYITNKSKVNDRERIELKKYCRWDR
                     KHTVHKETR"
BASE COUNT          573 a         1466 c         1672 g          728 t
ORIGIN      
        1 acgtgcacgt tgccgttgcg gtcggtgcgg tactcgacct tgccgccctt gaactcctcg
       61 acggccttgg ccgggtcggg ggtgacggtg ccggtcttgg ggttgggcat caggccgcgg
      121 gggccgagca cccggcccag tcggccgacc agcggcatca ggtccggcgt ggcgatcgtc
      181 acgtcgaagt ccagcatgcc gccctcgatc tgggcggcga ggtcctcggc gccgacgaac
      241 tcggcaccgg cggcctcggc ggcggtcgcc gcgtcgccct gggcgaacac ggcgatgcgg
      301 acgtccttgc cggtgcccga cggcagcgcg acggtgcccc ggatcatctg gtcggccttg
      361 cgggggtcga cgccgaggcg gacgaccagc tcgaccgact cgtcgaagtt ggcggtggcc
      421 agcgacttca cgacgtcgat cgcctcatcg gcggtgtggt gacgctcgcg gtcgaaacgc
      481 ttggtcgcgt cgaggtagcg ctttccgtgg gccatggtgc gaatccttcc ggttctccct
      541 catctgtgac ccgggtgccc gggcgaggtc ctcccgggtg gggttcgacg gccggagccg
      601 tcacgatcgg ttgggcccga cgccgagcac tctgcggcgg tcggtgcggg gccgtcgggc
      661 tcagacgacc tcgatgccca tggagcgggc ggtgccggcg atctgcagct tggcggcgtc
      721 gatgtcgtcg gtgttgaggt cggggagctt ggtctgggcg atctcggtca gctgggcgtc
      781 ggtgatcttg ccgacgaagc cgcgacccgg ggtctcggcc gccttgtcga gcccggccgc
      841 ctcacggatg agcaccgggg cgggcggggt cttggtgacg aaggagaacg tgcggtcctc
      901 gtagatcgag atctcgaccg gcacgatggt gccgcgctgg gcctcggtgc gggcgttgta
      961 ctccttgcag aagtccatga tcgccacgcc gtgcgggccg agcgccgtgc cgaccggcgg
     1021 cgcgggcgag gcctggccgg ccgggatctg gatcttgacg atggcgacga ccttcttctt
     1081 ggccatgact tcactttcct cggacttctg gacgggcccc ggagatggga cccggcggcg
     1141 ccccacgcgg ggagacgact cgactggggc acggcggcca cccgtcgggg cggcccggac
     1201 cgactgacca ttctggtccc gccccggagg cgggcacaac ccgagcgggc tggctcagag
     1261 cttggcgacc tgggcgaact cgagctcgac gggggtctcg cggccgaaga tgttgacgag
     1321 caccttgacc ttgagctggt cctcgttgat ctcgatgatc tcgccggaga agtcggcgaa
     1381 cggaccctcc ttgacccgga cggtctcgtt gatctcgtag ccgtggaccg gtccggccga
     1441 gcgcttgggg gtggccggtg cctcggcgtc gacctcttcg ccggtgccga ggaagttctc
     1501 gacgtccttg cggggcagcg cctggggctt gttggcggcg ccgacgaagc cggtcacccc
     1561 gggggtgttg cggatcacgt accagctgtc gtcgttcagg ctgcagcgga tcagcaggta
     1621 gccggggaac atcttcttct ggacgacgac cttcttgccc tgcttgaact cgacgacgtc
     1681 ctccatgggg atgacgacct cgtggatgcg ggactccatg ttcatcgagg cgcggcgggc
     1741 ctcgatgttc tggcggacct tgttctcgta gccggactgg gtgtgcagca cgtaccactt
     1801 gccggggcgg tcgtacggcg acaccggctt gggcgccggg ggctcctgcg cgtcgaacag
     1861 gtcgtcctcg tcgagaatgg cctcgtcctc gacgagctcg tcgaggacgg cgtcgtcctg
     1921 cacgggctcc tgcaccagca cgccgtcggc gtcgatgtcc tcggcgtcgg ccagggcctc
     1981 gatcgcgtcg gctccggcgt cgtcgccggc gacctcggtg atgagctcct ccacccgggc
     2041 ctcgtcgtcg accgcggcct ggagcgcgtc gtcggcggcc tccgtggtgg ggatgtcgtt
     2101 ctggtcagtc atggatctct ctcgggcgga gtgtcgggac cggctgcgtt cgtcgccgat
     2161 gccgttcgga tgctcggggt gggtcggagg gctgctgatc cgacctcagt acagggtggg
     2221 aggcgtcagc agcccggacg cgcccgaggt ggagttcccc gggtcgagga tgatccggaa
     2281 cagctccccg aacgcgtagt cgacgacgaa cacgccggcg gtgaagatga ccagggccac
     2341 cagcacgatc atcgagtagc ggatgacctc ctggcggtcg ggccaggaga ccttgcgcat
     2401 ctcggcgcgg acctcacgga cgaactcgag cggcttcgtg cgctcggact tcacgttctg
     2461 agccggcgcc ttgcggtcgc gcggtgcctg cgagccgtca gcgttcagct gcccggcgcg
     2521 ctgcatggca cgcttctgct ctcggttcat cgccatctgt gggggacctc ttcgacctgt
     2581 ggagcagggc aggagggact cgaaccccca accgccggtt ttggagaccg gtgctctacc
     2641 aattgagcca ctgccctgtg gcgcaacccg tccggaccgc acccccatcc gggccgacca
     2701 cgggtgtgtc ggagcatggg agtccagacc tcgcgagcga cgtcaagggg caagactaga
     2761 cgcgcggacg gccgggcacg actcgcgatt ccggagccgg tcgtccgggg gatcgtccgg
     2821 ggatcgtccg gacagctcac ccgcacgccg cggtgaccgg tcggcatgta ccggcgagag
     2881 tcgcgtcgct cagccggcca gcgacgggga tcgacgcgaa cggctgttga tcacgaccgc
     2941 tccgatcacc ccggccaccg tcgccgccgc cactccggcg ccgtaggcga cgcgtcgccg
     3001 ccgcagcagg gagcggacgg cgtggcgctc ccccacctcc tcgaccgcgg cgagcacgtc
     3061 ggtgagcatc cccggcgcgg gttcgagcac ctcggcccgc agcgacctca gggtgcgcag
     3121 cacccgacgg tgctggacca gctccgcctg gcagcgcagg cagtgctcga cgtgggcggc
     3181 catgtcggag ggcagcacga ccgtgccgtc ggacatggcc ggcagaagcg gagcgacctc
     3241 gtcacagcgc acggccgggc acctcggcga gctcggggaa gacgtcggag cgcagtcggt
     3301 gacgggcccg gtgcagtcgc accttggccg cggtcacgct gatgccgagc tcctcggcga
     3361 tggcgtcgtg gggaagctcg tacacgtcgc ggagcacgat cacggcgcgc agcttggggg
     3421 gcagctggcc cagcgcgacc aggatgcggt cgcgcaggtc gccggcatcg gcggccaggg
     3481 ccgggtcgtg gtcgggccgc gggtcagcga tgggcgagtc gtcggccagc tcgtcgtgac
     3541 ggtggcggcg gttccggccg gcctgggtgg cggccacgtt cgcggtgatc cggtacatcc
     3601 aggtggagaa gcgggcgtcg ccccggaacg agccgaggcc gcggtaggcc cgcaggtacg
     3661 cctcctgggt gacgtcgcgg gcgtcctcga cgcatccggt gaggtgcagg gccagggcgt
     3721 aggtgtcgcg gtgggtcgcc tcgaccaggc gggcgaacgc ggcgtggtca ccggagcggg
     3781 cggcgtcgac gtcggcggcc tgcgcatcgt cggacgcggg gtcctgctcg gagcgaccgc
     3841 tggacgggcg gggggccgag ccgagcagcc ggaggtccgg ggccgggctc tccggcggtg
     3901 ctggactcgc cggcgggccc gcgtcgccga ggagcagcgt ggcgtggggg tcgctcacgg
     3961 cgtcacccta ttcgctgcag cgatgcggat ccgggcaccc gacgcgcgtt ccgatgacac
     4021 ccaggtaccg aaagtggtgc tgacttgtcg tcagaacgcg gatccggggt ggatcagcgg
     4081 gtctccttgt ggaccgtgtg cttgcggtcc cagcggcagt acttcttgag ctcgatgcgc
     4141 tcgcggtcgt tcaccttgga cttgttggtg atgtagttgc gacgcttgca ctcggtgcac
     4201 tcgagggtca cagcgacccg cttgtcggag gccatcggct cccacttcct tgtcccgacc
     4261 cggtgctcga ccgggtcgca gttcgttcgt tgctggtgcc cccggggccc ggatgggccg
     4321 cgacggggac ctgtcagtgt gctccggccc cgacccggtg gacaagccga ccgggccggg
     4381 tccgggtcag gtcacttgag gatcttggtg acgcgacccg cgcccaccgt acgaccacc
//
LOCUS       DAOQOD010000227         4435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8628_length_4435_cov_11.521689, whole genome shotgun sequence.
ACCESSION   DAOQOD010000227 DAOQOD010000000
VERSION     DAOQOD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8628_length_4435_cov_11.521689"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PLZ72_14880"
     CDS             <1..1478
                     /locus_tag="PLZ72_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNA37630.1"
                     /translation="GGVLWARVEQGGHVNVYDNLYINGAWVPPAADKGYAEVIDSSTE
                     EVMAKVVQGGREDVDRAVAAARDAFTTWSTTPVDVRAKYLRDIGAGLTARFTEIAETI
                     SGEVGSPKAFAEMVQAGLSVGDWETYARIAEEFDWETQVGNSLVVREPIGVVGAVTPW
                     NYPLYLIVCKIGPALAAGCTIVLKPASYTPLNAIILTEVIDEVGLPPGVFNLVTGPGG
                     EVGEALAKHPDVDMVSLTGSGPVGAQVSKDAADTVKRVGLELGGKSANIVLDDADPAT
                     VAMGAVFGCFLNSGQTCSALTRVLVPRSREQEFVDAIVAEASQVRLVDPHADEPDIFH
                     ALGPMVSKSQQSTVLEYIQKGIDEGATPVVGGAGVPDGFDKGYYVKPTVFTGVTPDMT
                     IAQEEIFGPVLSVIAYEDDDDAVRIANDTVFGLAGAVQSGDVERAKSVARRLRTGQVD
                     INGAAFNTEAPFGGYKQSGNTRERGRWGLEEFLETKSIQLP"
     gene            complement(1576..2985)
                     /locus_tag="PLZ72_14885"
     CDS             complement(1576..2985)
                     /locus_tag="PLZ72_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNA37631.1"
                     /translation="MSASDVNESEVIAELLAALGPTGVITDADVAMPYRFDRAATAEP
                     GRPLAVVRPSSTAEVQATVRIAAAHRVPIVTRGAGTGLSGGSTAVDGAITLSTDRMRA
                     VRVDTAAQLAVVQPGALNVEVKAAAAREGLWYPPDPSSFEICSIGGNLATNAGGLCCV
                     RYGVTTDYVLGLEVVLADGRAVRLGGRTVKDVAGYDLKRLFVGSEGTLGIVTEATLRL
                     RPPPPPVTTVVATFDDVTDAGRAVSAVMSSMRPAALELMDAAAVAAVERVRPMGLAGV
                     GALLLAQCDTPSPGSAASSGHAAASGDGAELARCFGDAGANEVAVSTDADEGEMLMGA
                     RRMAIPCVERLGTVMIEDVGVPIPAIPDLVAAVAAISERTRTAIPVIGHAGDGNFHPL
                     VTFDPTDPDAVERARGAFDDVMRAAVELGGTITGEHGVGSLKAAHLGAQLGPDVMELN
                     RRIKDALDPDGILNPGKWI"
     gene            complement(3032..>4435)
                     /locus_tag="PLZ72_14890"
     CDS             complement(3032..>4435)
                     /locus_tag="PLZ72_14890"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNA37632.1"
                     /translation="AAPSSVGRQMTFRTIIEPFRIHSVEPLRMTTPDERRAALDAAGH
                     NLFSLRAEDVLIDLLTDSGTGAMSRDQWAGIQRGDESYAGSPSWFRFLDAVQDLFPFQ
                     HVIPTHQGRAAEKILFSVLGGDGRVVPNNTHFDTTRANVEFTGARAVDLVIAEGRDPS
                     STHPFKGNMDLDALDALLAEHGPERVPVVFVTVTNNSGGGQPVSLENLRGVRAVCDRH
                     GVPLFLDACRFAENAWFIREREPGQADRDVADIVREMASLADGMTMSAKKDPLGNIGG
                     WLAMNDSELAERCRTILIITEGFPTYTGLAGRDLEALAQGLHEAVDHDYLRYRIRSTA
                     YLGEALVAAGVPVVQPIGGHAVYLDAGALLGHLDPLAFPGQSLAVALYEEGGVRGCEI
                     GSVMFGLQPDGTEQAAAMELVRLAIPRRTYTQSHIDYVIEVCERVAARADELPGYRIT
                     SDPRPLRHFTATFEPIP"
BASE COUNT          649 a         1545 c         1620 g          621 t
ORIGIN      
        1 ggggaggggt actgtgggcc cgagtcgaac aagggggaca tgtgaacgtc tacgacaacc
       61 tgtacatcaa cggggcctgg gtgccgccgg cggccgacaa gggctacgcc gaggtcatcg
      121 actcctccac cgaagaggtc atggccaagg tcgtccaggg aggccgcgag gacgtcgacc
      181 gcgccgtcgc cgcggcccgc gacgccttca ccacctggtc gaccaccccg gtcgacgtgc
      241 gggccaagta cctgcgcgac atcggcgccg gcctgaccgc ccgcttcacc gagatcgccg
      301 agacgatctc cggcgaggtt ggctccccca aggccttcgc cgagatggtc caggccggtc
      361 tgtcggtcgg cgactgggag acctacgccc gcatcgccga ggagttcgac tgggagaccc
      421 aggtcggcaa cagcctggtg gtgcgcgagc ccatcggcgt ggtcggcgcg gtgacgccgt
      481 ggaactaccc gctgtacctg atcgtctgca agatcggacc cgccctggcc gccggctgca
      541 cgatcgtgct caagccggcc agctacaccc cgctcaacgc catcatcctc accgaggtca
      601 tcgacgaggt gggcctgccg ccgggcgtgt tcaacctggt caccggcccc ggcggcgagg
      661 tcggcgaggc gctggccaag cacccggacg tggacatggt gtcgctcacc ggttccggcc
      721 ccgtcggcgc ccaggtgtcc aaggacgcgg ccgacacggt caagcgcgtc gggctggagc
      781 tcggcggcaa gtcggccaac atcgtgctcg acgacgccga tccggccacc gtggccatgg
      841 gggcggtctt cggctgcttc ctcaactcgg gccagacgtg ctcggcgctc acccgggtgc
      901 tcgtgccccg ctcccgtgag caggagttcg tcgacgccat cgtcgccgag gcctcgcagg
      961 tgcgtctggt cgacccgcac gcggacgagc ccgacatctt ccacgccctc ggcccgatgg
     1021 tgtcgaagtc ccagcagagc acggtgctcg agtacatcca gaagggcatc gacgagggcg
     1081 ccacgccggt cgtcggcggc gccggcgtcc ccgacggctt cgacaagggc tactacgtca
     1141 agccgacggt gttcaccggc gtcacgcccg acatgacgat cgcccaggag gagatcttcg
     1201 gtccggtgct gtcggtcatc gcctacgagg acgacgacga cgccgtgcgc atcgccaacg
     1261 acaccgtgtt cggcctggcc ggagcggtgc agtccggcga cgtcgagcgg gccaagtcgg
     1321 tcgcccggcg gctgcggacc ggccaggtcg acatcaacgg cgcggcgttc aacaccgagg
     1381 cgccgttcgg cggctacaag cagtcgggca acacccgtga gcgcggccgc tggggcttgg
     1441 aggagttcct cgagaccaag tcgatccagc tcccctagaa attcgggtgc tcagcgacac
     1501 accttgtcgc aatcccgacc tcagaacttc ggcggtgccc gggcccctcg gtccgggcac
     1561 cgtcgcgctc gggcgtcaga tccacttgcc ggggttgagg atcccgtcgg ggtcgagggc
     1621 gtccttgatg cgacggttga gctccatcac atccggtccc agctgcgcgc ccaggtgggc
     1681 ggccttgagc gagccgaccc cgtgctcgcc agtgatcgtc ccgcccaact cgacagcggc
     1741 gcgcatcacg tcgtcgaacg cgccccgggc ccgttcgacc gcgtccgggt cggtcgggtc
     1801 gaaggtgacc agcgggtgga agttgccgtc gccggcgtgg ccgatcaccg ggatggcggt
     1861 gcgggtgcgc tcagagatgg cggcgaccgc ggcgaccagg tcggggatgg cggggatggg
     1921 gaccccgacg tcttcgatca tgacggtgcc gagccgttcg acgcacggga tcgccatgcg
     1981 tcgggcgccc atcagcatct cgccctcgtc cgcatcggtg ctgacagcga cctcgttcgc
     2041 tccagcgtcg ccgaagcagc gcgccagctc ggcgccgtcg ccggatgccg cggcgtggcc
     2101 ggatgatgct gcgctcccgg gagagggcgt gtcgcactgg gcgagcagca acgcgccgac
     2161 gccggccagg cccatcggac gaacccgttc gaccgcggcg accgcggcgg cgtccatcag
     2221 ctcgagcgcg gcgggtcgca tggacgacat gacggcgctg accgcccggc cggcatcggt
     2281 gacgtcgtcg aaggtggcga ccaccgtggt caccggcggc ggcggcggac gcagccgaag
     2341 ggtcgcctcg gtcacgatcc cgagggtgcc ctcggatccg acgaacaggc gcttgaggtc
     2401 gtagccggcg acgtccttga cggtccgccc gccgagccgg accgctcgac cgtcggcgag
     2461 caccacctcg aggcccagca cgtagtcggt ggtcacgccg tagcgcacgc agcacagccc
     2521 gccggcgttg gtggccaggt tcccgccgat cgagcagatc tcgaacgacg acggatccgg
     2581 cggataccac aggccctccc gggcggcggc ggccttgacc tcgacgttga gcgcgccggg
     2641 ctgcactacg gcgagctgcg cggcggtatc gacacgcacc gctcgcatcc ggtcggtgga
     2701 cagggtgatg gccccgtcca cagcggtcga cccgcccgac agcccggtgc ccgcgccgcg
     2761 ggtcacgatc ggcacgcggt gcgcggcggc gatgcggacc gtggcctgca cctcggcggt
     2821 gctcgacggg cgcaccaccg ccaggggtcg gcccggctcg gcggtggcgg cccggtcgaa
     2881 gcggtacggc atggcgacgt ccgcgtcggt gatcacgccg gtcgggccga gagccgcgag
     2941 cagctccgca atcacctcgg actcgttcac gtcggacgcg ctcacctcgg actcgtcgac
     3001 atcacagacc cggtcacgtt cggcggtccg ctcaggggat cggctcgaag gtggcggtga
     3061 agtgccgcag cgggcgggga tcggaggtga tccggtagcc gggcagctcg tcggcccgtg
     3121 ccgcgacccg ctcgcacacc tcgatcacgt agtcgatgtg gctctgggtg taggtgcgtc
     3181 gggggatggc caggcggacc agctccatgg ccgcggcctg ctcggtgccg tcgggctgca
     3241 ggccgaacat gaccgagccg atctcacatc cccgcacccc accctcctcg tacagcgcca
     3301 cggcgagcga ctggcccggg aaggcgagcg gatcgaggtg gccgagcagg gcgccggcgt
     3361 cgaggtagac ggcgtgaccg ccgatcggtt gaaccaccgg aaccccggcc gcgaccagcg
     3421 cctcgcccag gtaggcggtc gagcggatcc ggtagcggag gtagtcgtgg tcgaccgcct
     3481 cgtgcaggcc ctgcgccagc gcctcgaggt cacgtccggc caggccggtg taggtgggga
     3541 acccctcggt gatgatgagg atggtgcggc accgctcggc cagctccgag tcgttcatgg
     3601 ccagccaccc gccgatgttg cccagcgggt ccttcttggc gctcatcgtc atgccgtcgg
     3661 ccagcgacgc catctcgcgc acgatgtcgg ccacgtcgcg gtcggcctgg ccgggctcgc
     3721 gctcgcggat gaaccaggcg ttctcggcga accggcaggc gtcgaggaac agcggcaccc
     3781 cgtggcggtc gcagacggcg cggacgccgc gcaggttctc gagcgacacg ggctggccgc
     3841 cgccggagtt gttggtgacg gtgacgaaca cgaccggcac ccgctcgggc ccgtgctcgg
     3901 cgagcagcgc gtcgagcgcg tcgaggtcca tgttgccctt gaacgggtgg gtcgaggacg
     3961 ggtcgcgacc ctcggcgatg accaggtcga cggcccgggc gccggtgaac tcgacgttgg
     4021 cgcgggtggt gtcgaagtgc gtgttgttgg ggacgacccg gccgtcgccg ccgagcaccg
     4081 agaacaggat cttctccgcg gcccggccct ggtgggtggg gatgacgtgt tggaacggga
     4141 acaggtcctg cacggcgtcg aggaagcgga accacgacgg cgacccggcg tagctctcgt
     4201 cgccgcgctg gatgccggcc cactggtccc tcgacatggc gccggtgccc gagtcggtga
     4261 gcaggtcgat gagcacgtcc tcggcccgca gcgagaacag gttgtgcccg gcggcgtcga
     4321 gcgcggcgcg gcgctcgtcg ggagtggtca tccgcagcgg ctcgaccgag tggatgcgga
     4381 acggctcgat gatggtgcgg aacgtcatct gccgcccgac gctactcggg gcggc
//
LOCUS       DAOQOD010000228         4358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8879_length_4358_cov_9.845224, whole genome shotgun sequence.
ACCESSION   DAOQOD010000228 DAOQOD010000000
VERSION     DAOQOD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8879_length_4358_cov_9.845224"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1538
                     /locus_tag="PLZ72_14895"
     CDS             78..1538
                     /locus_tag="PLZ72_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNA37633.1"
                     /translation="MSARADLKDLRRHRVPAWWSDAKLGIFVHWTPASVAGFAPIDSH
                     IGDLLAAGDPEALAENPYTEWYENSLRFPNSSASRYHRETYGLMPYAELADLYREGLN
                     SWDPDDWARTFAATGARYVVLVTKHHDGFCLWPSSVTNPNRPGWHTGRDVVGELSEAV
                     RAAGMRFGVYYSGGLDWTFDDRPIGNLGSMLAAIPRGSYPDYAAAQVRELIERYRPSV
                     LWNDIAWPAPGKDLWPLLAEYYRAVPDGVVNDRWMPWSPVMAAARNDLVSRGADAINA
                     RATRSKGGLVPPKPPFFDVQTPEYVVFDDVRRTPWECVRGMDQSFGYNRMSGPEQFLT
                     DDELLDMAADITSKGGNLLLNVGPRGEDAAIPDEQRARLDTLARWAGHGPGSAMSGSR
                     PWVRPSGEGVVGDGSRVPLRYWAADRHVDVAVLGPLDGSLLVDDVEATPTTSVIDAAS
                     GEQLVWNATDEGIRVEVGGPADRPVLRLTDVVARPS"
     gene            1679..2401
                     /locus_tag="PLZ72_14900"
     CDS             1679..2401
                     /locus_tag="PLZ72_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37634.1"
                     /translation="MSSNRRMGVAAAVLLGSGLILMIVPPVVRDGLCGLTSCADRVPV
                     IAVSRVSPTELAVVVPDDAAPNVRRVELLQGGSSGSGPRQWMIRAERGSDTGSNPDSG
                     SGGAISARTFLIGDEPDGFRTVVPLETEPQKGVWTAQVGFRCTTASLPFNPQALAVGE
                     VRSWDGVMDGNDFAADANTSEHCLTERGTAELGLLLAGAALAVAGAVVGIVVVLRRPA
                     RFPEDGDDPAAASERDQDPDRV"
     gene            complement(2441..>4358)
                     /locus_tag="PLZ72_14905"
     CDS             complement(2441..>4358)
                     /locus_tag="PLZ72_14905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNA37635.1"
                     /translation="RAAPLAENCRAAAGRLDDAHPWAIGLTRPFNQDGFGSDSRGHVD
                     ALLRRRSAAVGLALWEPNATLVRTQDLRRHPIDPRRPRGAWLRARHAEGGRPATVDQP
                     LALVAAPAGARTFWPESMAQQRGSAADAADAVRSGSGTARRVALGLLVRDCFAWSMLT
                     WLLVLFTGTVSGELPMRTANGAFPALVLAALLLRWVGLYRSAGRTPHPFRDLRATVDD
                     VPGSLAALPSAITGRVGRGPSRVAVRPLLWAGLLSVAVLVTALVDHSPDQPMTAPAVG
                     AALLTLVLLWVVCIQVLVQRGWERTSFRVPLSRPARLDSRDARTVDVSPEGLAVELTR
                     VGSGDTPGDPATGDGSGQATSGLPAIGSTTRVGVELDDGSSTDMDATVMWTHHGHRRD
                     LVGLRLELDESSRPQWAGLVLHAAETAAGVQLAAEPGRTTRSPRTTHSTARSHSPRTT
                     RSTDRTPGPAATATAGVSRRSRALDVVGLGATVLLSIVLLAVLGGAMLGLQAAVVRSG
                     SMTPTITQGSVVVSESVPVSSLRPGDVVTRPAEGAAEPVTHRLVSATRQGDTYLMQTR
                     GDANESGETWTVPADSTLPRIRWVVPSIGDGIAVLRSHLVLVLGLVVIGGLLIAALRL
                     PARRHRPTMTPASA"
BASE COUNT          603 a         1578 c         1638 g          539 t
ORIGIN      
        1 ccgctttgta gtgcgagatc caggcgaacg cctggatctc gcactacaga acgggtggat
       61 cgggcaggat gcccgacatg agcgcccgag ccgacctgaa ggacctgcga cgtcaccggg
      121 tgccggcgtg gtggtccgac gccaagctgg ggatcttcgt gcactggacc ccggcgtcgg
      181 tggcggggtt cgctccgatc gactcccaca tcggcgacct gctcgcggcc ggtgaccccg
      241 aggcgctggc ggagaacccc tacaccgagt ggtacgagaa ctcgctgcgg ttcccgaaca
      301 gctcggcgag ccggtaccac cgcgagacct acggcctcat gccgtacgcc gagctggccg
      361 acctctaccg cgagggcctg aacagctggg accccgacga ctgggcccgg accttcgcgg
      421 cgaccggcgc ccgctacgtg gtcctggtca ccaagcacca cgacgggttc tgcctgtggc
      481 cgtcgtcggt caccaacccg aaccgcccgg gctggcacac cggccgcgac gtcgtcggcg
      541 agctgagcga ggcggtgcgc gccgccggga tgcgcttcgg cgtctactac tccggcggcc
      601 tcgactggac cttcgacgac cgcccgatcg gaaacctcgg ctccatgctc gcggccatcc
      661 cccgcggctc gtacccggac tacgcggcgg cgcaggtccg tgagctgatc gagcggtacc
      721 gacccagcgt cctgtggaac gacatcgcct ggccggcgcc gggcaaggac ctgtggccgc
      781 tgctcgccga gtactaccgg gccgtgcccg acggggtggt caacgacagg tggatgccgt
      841 ggtcgccggt catggccgcg gcccgcaacg acctggtctc ccgtggggcc gacgcgatca
      901 acgcccgcgc cacccgctca aagggcggcc tcgtcccgcc caagccgccg ttcttcgacg
      961 tccagacccc cgagtacgtg gtgttcgacg acgtccggcg gacaccgtgg gagtgcgtcc
     1021 ggggcatgga ccagagcttc ggctacaacc ggatgtcggg cccggagcag ttcctgaccg
     1081 acgacgagct gctcgacatg gccgccgaca tcacctccaa gggcgggaac ctcctgctca
     1141 acgtcggccc ccgcggcgag gacgccgcca tccccgacga gcaacgcgcc cgactcgaca
     1201 ccctcgcccg atgggccgga cacggaccgg gctcggccat gtccgggtcc cgtccgtggg
     1261 tgcggccctc gggcgagggc gtcgtcgggg acgggtcccg ggtgccgctg cggtactggg
     1321 cggccgaccg gcacgtcgac gtcgccgtcc tgggaccgct cgacggttcg ctgctcgtgg
     1381 acgacgtcga ggcgactccg acgacgtcgg tgatcgacgc ggcgtcggga gagcagctgg
     1441 tgtggaacgc gaccgatgag gggatccggg tcgaggtcgg cggcccggcg gaccgaccgg
     1501 tcctccgact caccgacgtg gtcgcccgcc cgagctgaca gcggagcgac cgagcggctg
     1561 agcgtccgag cgtccgagcg actgagcgtc gaggcccgcg accgccgggg accgggacgg
     1621 tgacagccga ccgcggcggc gcccgccgcg tcgccggcgc aacccctacc atcacggcgt
     1681 gagctccaac cggcggatgg gagtggccgc ggcggtgctg ctgggttccg ggctgatcct
     1741 catgatcgtc ccgccggtgg tgcgggacgg cctctgcgga ctcacgtcgt gcgcggaccg
     1801 ggtgccggtc atcgctgtgt cgagggtgtc gccgaccgag ctggccgtcg tcgtccccga
     1861 cgacgcggcc ccgaacgtgc gccgggtcga gctgctccag ggtggatcga gcgggtcggg
     1921 cccccgccag tggatgatcc gcgccgagcg tggttccgac accggctcca acccggacag
     1981 cggctccggc ggcgccatct cggcacgcac cttcctcatc ggcgacgagc ccgacgggtt
     2041 ccgcaccgtg gtcccgctgg agaccgaacc gcagaagggg gtgtggaccg cccaggtcgg
     2101 gttccgctgc accacggcgt cgctgccgtt caacccccag gccctcgccg tcggcgaggt
     2161 ccgatcgtgg gacggcgtca tggacggcaa cgacttcgcc gcggacgcca acaccagcga
     2221 gcactgcctc accgagcgag gtacggccga gctgggactg ctgctggccg gagcggctct
     2281 cgccgttgcc ggtgccgtgg tgggcatcgt ggtggtcctg cgccgaccgg cccgcttccc
     2341 cgaggacggc gacgacccgg ccgccgcctc cgagcgcgac caggacccgg accgggtctg
     2401 atcgacccgg cgaccccggt ctccgtcggg cggtcgccgc tcaggccgag gccggggtca
     2461 tcgtggggcg gtgtcgtcgg gccgggagcc gcaacgcggc gatcagcagc ccgccgatga
     2521 ccaccagccc gagcaccagc accagatgcg agcgcagaac tgcgatgccg tcgccgatgc
     2581 tgggcacgac ccaacggatc ctcggcaggg tgctgtccgc cggcacggtc cacgtctcgc
     2641 cggactcgtt ggcgtcgccc cgggtctgca tcaggtaggt gtcgccctgt cgggtcgcgg
     2701 agaccagccg gtgggtgacc ggctccgccg cgccctcggc cggtcgggtg accacgtcgc
     2761 cggggcgcag cgacgacacc ggcaccgact cgctgaccac caccgacccc tgggtgatcg
     2821 tcggggtcat cgacccgctg cggaccactg ccgcctgcaa cccgagcatg gcaccgccca
     2881 ggacggccag cagcacgatc gacaacagga cggtcgctcc gaggccgacc acgtcgagcg
     2941 cccgggaccg acgggagacc ccggcggtgg cggtggctgc cggtccgggc gtccggtcgg
     3001 tggagcgtgt cgtgcggggg gagtgtgacc gggcggtgga gtgcgtcgtg cggggggagc
     3061 gagtcgtcct gccgggctcg gcggccagct gcacgccggc tgccgtctcg gcggcgtgca
     3121 gcacgagccc cgcccactgc gggcggctcg actcgtccag ctccaggcgc agtccgacca
     3181 ggtcacggcg gtggccgtgg tgggtccaca tgacggtggc gtccatgtcg gtcgaggagc
     3241 cgtcgtcgag ctcgacgccg acccgggtcg tggaaccgat cgcggggagt ccggaggtcg
     3301 cctgacctga tccgtcgccg gtcgcggggt ctccgggcgt gtcgccggag cccactcggg
     3361 tcagctcgac ggccaggccc tccggcgaca cgtcgaccgt gcgggcgtcg cgggaatcca
     3421 ggcgtgccgg tcggctgagc gggacccgga acgaggtgcg ctcccagccc cgttgcacca
     3481 ggacctggat gcacaccacc cacagcagca ccagggtgag cagtgcggcg ccgacggcgg
     3541 gggcggtcat cggctggtcg ggggagtggt cgaccagggc cgtcaccagc accgcgacgc
     3601 tcagcagccc ggcccacagc agcggccgga cggccacgcg cgacggccct cgaccgaccc
     3661 ggccggtgat cgccgagggc aacgcggcca acgacccggg aacgtcgtcg accgtggcgc
     3721 gcaggtcgcg gaacggatgc ggggtgcgac cggcggaccg gtacagcccg acccagcgca
     3781 gcagcagcgc ggcgagaacc agggcgggga aggcgccgtt cgctgtgcgc atcgggagct
     3841 cccccgacac ggtgccggtg aacagcacga gcagccaggt cagcatcgac caggcgaagc
     3901 agtcccggac gagcagaccc agggcgaccc gccgagccgt gccactgccg gagcggacgg
     3961 cgtccgccgc gtcggcagcg gacccgcgct gctgggccat cgactcgggc cagaaggtgc
     4021 gggcgccggc gggggcggcc accagagcga gcggctggtc gacggtggcc ggccgaccgc
     4081 cctcggcgtg gcgggcacga agccacgctc cgcgcgggcg gcgggggtcg atcgggtggc
     4141 gccgcaggtc ctgggtgcgc acgagcgtgg cgttgggctc ccacagggcg agcccgaccg
     4201 cggcgctgcg ccggcggagg agcgcgtcga cgtggccccg gctgtcggac ccgaacccgt
     4261 cctggttgaa cggtcgggtg aggccgatcg cccaggggtg ggcgtcgtcg agtcgaccgg
     4321 ccgcggctcg gcagttctcg gccagcggcg cggcccga
//
LOCUS       DAOQOD010000229         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_8962_length_4332_cov_10.800327, whole genome shotgun sequence.
ACCESSION   DAOQOD010000229 DAOQOD010000000
VERSION     DAOQOD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8962_length_4332_cov_10.800327"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLZ72_14910"
     CDS             <1..244
                     /locus_tag="PLZ72_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37636.1"
                     /translation="IVMSRAAVGLALVLAVVGMMVGGTIGSSAATAAVAVVLIAPLAR
                     VVWLAARWFRRGDPRYGAVALGVLAVVAGAAVLAAI"
     gene            complement(241..1446)
                     /locus_tag="PLZ72_14915"
     CDS             complement(241..1446)
                     /locus_tag="PLZ72_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNA37637.1"
                     /translation="MSDAVAGDYTLIEDDAAFADLLDRLDSQDRYAIDTEFHRERTYF
                     PHVALVQIADRHGVALVDSLQVDLAPFARILDSDATAVMHAARQDMEVLERSTGTVPA
                     RLVDTQIAAGFLGYTSPSLATLLERELKVRAPKADRLTDWLRRPLTPNQLDYAASDVA
                     HLLELFDTLWSRLEARGRDVWALDACEELRTEPRGPRDPDDAWRRIKELRHLRGSTLA
                     VAQAVAGWREERAIETDQTPRFVLSDLGVVSVASATPSSVDELRSLRGIDGRSIRGGM
                     GDELLAVVAAAAGTRPRRTPAPTAPELPGELRPAVPLISAWVSQLSRELELETSMLAT
                     RSDLEDLLRGVPGARLTHGWRAELVGEPIRRLLAGEAALAFDRRQGLVLEDRGVGNGQ
                     TDNSQADNS"
     gene            complement(1460..1780)
                     /locus_tag="PLZ72_14920"
     CDS             complement(1460..1780)
                     /locus_tag="PLZ72_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNA37638.1"
                     /translation="MSVPEITVDQLDEARGAGAFVLDVRNPEELAEARVPGVVPLPLP
                     ELETRADEVPSDVTVYVICRSGARSAQAVEFLRGRGVDAVNVAGGTMAWIDSGRGVDS
                     GPDA"
     gene            complement(1790..2461)
                     /locus_tag="PLZ72_14925"
     CDS             complement(1790..2461)
                     /locus_tag="PLZ72_14925"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNA37639.1"
                     /translation="MTSREARSGPAGVVLCGGSSTRMGRDKATMSTPNGPLAGVAARA
                     LQDAGCDPVVLVGGDGGALRSRLGLRWIADSEPGAGPLGGIASAADHLAGRALIVSAC
                     DLPDLRGSDVSPMRDAVRSSDAEVAVYDRDGRPQWSVLALGPAAATAMAAAFAAGERA
                     LHRAVATVAAAGLVVVHLDPPRPEGIGDVDRVEDLPDGWPAMPPESNPALDRIPDPAP
                     GQASG"
     gene            complement(2466..3728)
                     /locus_tag="PLZ72_14930"
     CDS             complement(2466..3728)
                     /locus_tag="PLZ72_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA37640.1"
                     /translation="MTFPTHGRPAGEILSEVASERSDDLDWRGGKAFSLVYNVDDPDL
                     EALQHEIASSFLHENALNPFRYRTLLRMEAEVVDMACSLFGAATGSMSSGGTESIFLA
                     VQTARDHARARGVTAPTLVCAATAHPAFAKACKYLDVRQVRLPTRSDGRADPDRFASA
                     IDADTALVVVSAPCYPFGVIDPVTEIAALAAERDVLCHVDACLGGYLLPWWERLGEPV
                     PPWDFRVPGVTSLSADIHKYGYAYKGASTVLYRDRDLYQRQIFMYDDWPGGLYASSTA
                     AGTRPGPPIAGAWATMTHLGEEGYLRLAERVRTAMRGFTAGIAGIDGVRITSDPDLSL
                     FEFGIDPDAEPDTGHGNGPLDIGGIADAMDDRGWNLDRQDGGLHVMLSPGHDKVIDAF
                     TADLADSVAHHQSDRGGDHVYGGVVDRA"
     gene            3806..4180
                     /locus_tag="PLZ72_14935"
     CDS             3806..4180
                     /locus_tag="PLZ72_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37641.1"
                     /translation="MLDSGGVSRLSRRRPDTSALISVLQRDGWWPPFVPSVVLVEATS
                     GRPDRDAYVNRFLETCDLVSELPEPIARRAAALRARAGRGSAVDAVAVALAEPFGAVL
                     TGDLQDLRALADHAEGMVVVRA"
BASE COUNT          544 a         1509 c         1667 g          612 t
ORIGIN      
        1 gatcgtgatg tcgcgcgccg cggtcggcct cgccctggtc ctggcggtcg tcgggatgat
       61 ggtcggcggg accatcggct cctccgcggc gaccgcggct gtcgcggtcg tgttgatcgc
      121 gcccctggcc cgcgtggtgt ggctggcggc gcggtggttc cgacgcggag atccccgcta
      181 cggggcggtg gcgctcggcg tcctggcggt cgtggccggt gccgcggtgc tcgccgcgat
      241 ctagctgttg tcggcctggc tgttgtcggt ctggccattg ccgacgccac ggtcctcgag
      301 gaccaggccc tggcggcggt cgaaggccag ggcggcctct ccggccagca gccggcggat
      361 gggctcgccg accagctcgg cgcgccaccc gtgggtgaga cgggcgccgg ggaccccgcg
      421 cagcaggtct tccaggtccg agcgggtggc gagcatcgac gtctcgagct ccagctcccg
      481 tgacagctgg ctgacccagg ccgagatcag cgggaccgcc ggtcggagct cgcccggcag
      541 ctcgggtgcg gtgggtgcgg gggtccgtcg gggccgggtg ccggccgcgg cggcgaccac
      601 cgccagcagc tcgtcgccca tcccgccgcg gatgctgcga ccatcgatcc cgcgcagcga
      661 ccgcagctcg tcgaccgagc tcggcgtcgc gctcgccacc gacacgacac ccaggtccga
      721 caggacgaac cggggtgtct ggtcggtctc gatcgcccgc tcctcgcgcc agccggccac
      781 ggcctgggcc accgccaggg tgctgccccg gaggtggcgc agctccttga tgcggcgcca
      841 ggcgtcgtcg ggatcgcggg ggccgcgggg ctcggtccgc agctcctcgc aggcgtcgag
      901 agcccagacg tcgcgccctc gggcctcgag gcgggaccac agcgtgtcga acagctcgag
      961 caggtgggcg acgtcggacg ccgcgtagtc gagctggttc ggcgtcaacg gtcgacgcag
     1021 ccagtcggtc agccggtcgg ccttgggggc ccggaccttc agctcccgct ccagcaacgt
     1081 ggcgagcgac gggctggtgt agcccaggaa ccccgccgcg atctgcgtgt cgacgaggcg
     1141 ggccgggacg gtgccggtgc ttcgctcgag cacctccatg tcctgacggg cggcgtgcat
     1201 gaccgcggtg gcgtcgctgt ccaggatccg ggcgaagggc gcgaggtcga cctgcagcga
     1261 gtcgaccagc gcgacgccgt ggcggtccgc gatctgcacc agggcgacgt gcgggaagta
     1321 ggtgcgctcc cggtggaact ccgtgtcgat ggcgtagcgg tcctggctgt cgaggcgatc
     1381 gaggaggtcg gcgaacgccg cgtcgtcctc gatcagcgtg tagtcgccgg cgaccgcgtc
     1441 ggacacgggc ggctgcggtt caggcgtccg ggccggagtc gacgccccga ccggagtcga
     1501 tccaggccat ggtcccaccg gcgacgttga cggcgtcgac gcccctgccc ctcaggaact
     1561 cgaccgcctg ggcgctgcgg gcgccgctgc ggcagatcac gtagacggtg acgtcggagg
     1621 ggacctcgtc ggcccgggtc tcgagctcgg gcagcggcag cgggacgacg ccgggcacgc
     1681 gggcctcggc gagctcctcg gggttgcgca cgtcgaggac gaacgctccc gctcctcggg
     1741 cctcgtccag ctggtcgacg gtgatctcgg gtacggacac gtcggcaacc tacccgctgg
     1801 cctgtcccgg ggcgggatcg gggatccgat cgagggcagg gttcgactcc gggggcatcg
     1861 caggccaccc gtccggcagg tcctcgacgc ggtccacgtc accgatcccc tccggccggg
     1921 gcggatcgag gtgcacgacg accagcccgg cggcggcaac ggtggcgacg gcgcggtgca
     1981 gcgcacgctc ccccgccgcg aaggcggcgg ccatcgcggt ggccgcagcc gggccgagcg
     2041 cgagcaccga ccactgcggc ctgccgtcgc ggtcgtacac ggccacctct gcgtcgctgg
     2101 agcggaccgc gtcccgcatg ggcgagacgt cggacccgcg gaggtcgggg aggtcgcacg
     2161 cgctgacgat cagggcacgc ccggccaggt ggtcggccgc ggaggcgatc ccacccagcg
     2221 ggcccgctcc gggctcggag tccgcgatcc agcggaggcc gagccgcgag cgcagggccc
     2281 cgccgtcacc gccgaccagc acgacggggt cgcaccccgc gtcctgcagc gcccgtgcgg
     2341 cgactccggc gagcggcccg ttcggggtcg acatggtcgc cttgtcccgg cccatgcggg
     2401 tggacgaccc accgcagagg acgaccccgg ccggtccgga ccgggcctct cgggaggtca
     2461 tcgcctcagg cgcggtcgac gacgccgccg tacacgtggt ccccaccccg atcggactgg
     2521 tggtgggcca ccgagtcggc caggtcggcg gtgaacgcgt cgatcacctt gtcgtggccg
     2581 ggcgacagca tcacgtgcag gccgccgtcc tggcggtcca ggttccagcc ccgatcgtcc
     2641 atggcgtcgg cgatgccgcc gatgtccagc gggccgttcc cgtggccggt gtcgggctct
     2701 gcgtcggggt cgatgccgaa ctcgaacagc gacaggtccg gatccgaggt gatgcgcaca
     2761 ccgtcgatgc cggcgatgcc ggcggtgaag ccccgcatgg cggtgcggac ccgttcggcc
     2821 aggcggaggt agccctcctc gcccaggtgc gtcatggtcg cccatgcccc cgcgatcgga
     2881 ggcccggggc gggtgccggc cgccgtcgac gaggcgtaca ggccgccggg ccagtcgtcg
     2941 tacatgaaga tctggcgctg gtagaggtcg cggtcgcggt acaggacggt cgacgctccc
     3001 ttgtaggcgt agccgtactt gtggatgtcg gccgacagcg acgtgacacc ggggaccctg
     3061 aagtcccacg gcggcaccgg ttcgcccagg cgttcccacc agggcagcag gtagccgccc
     3121 aggcaggcgt cgacgtggca caggacgtcg cgctccgcgg cgagtgcggc gatctcggtg
     3181 accgggtcga tgaccccgaa cgggtagcac ggggcagaga ccacgaccag ggcggtgtcg
     3241 gcgtcgatcg cgctggcgaa gcggtcggga tcggcccgac cgtcggaccg ggtcggcagg
     3301 cgcacctggc ggacgtcgag gtacttgcac gccttggcga acgccggatg ggcggtggcc
     3361 gcgcacacga gggtgggcgc ggtgaccccg cgggcccggg cgtggtcccg cgcggtctgc
     3421 accgccagga agatcgactc ggtcccgccc gaggacatcg aaccggtggc ggcgccgaac
     3481 agcgagcagg ccatgtcgac gacctcggcc tccatgcgca gcagggtccg gtagcggaag
     3541 gggttcagcg cgttctcgtg caggaagctg gacgcgatct cgtgctggag ggcctcgagg
     3601 tcggggtcgt cgacgttgta gacgaggctg aacgccttgc cgcctcgcca gtcgaggtcg
     3661 tcggaccgtt ccgatgccac ctcggagagg atctcgccgg cgggtcgtcc gtgcgtcggg
     3721 aacgtcatgg atcgacgtta gcgccgcgcc agagcgcgac cgccgggcga tcgcagggcg
     3781 gtgctgccct ggccggagcg aacccgtgct cgattccggg ggcgtcagcc gcctctcccg
     3841 gcgacgcccc gacacctctg ccttgatctc ggtccttcag cgcgacggat ggtggccgcc
     3901 gttcgttccc agtgtggtgc tggtcgaggc gacatccggc cgtccggacc gggatgcgta
     3961 cgtgaatcgc ttcctcgaga cctgcgacct ggtcagcgaa ctgcccgagc ccatcgctcg
     4021 gcgtgccgcg gcgctgcgcg ctcgggctgg tcggggctcc gctgtcgatg ccgtcgccgt
     4081 ggcgctcgcc gagccgttcg gggccgtgct caccggcgat ctccaggatc tccgcgcact
     4141 cgcggaccat gctgagggaa tggttgtcgt gagggcatga tccaggtccg ctccgcctgg
     4201 tccccggcgg ctgacgcggt gttgcccgcg ctctggcggt ggatgatgcc gtggtggtga
     4261 cggcacagca gggtgaggtt ggtggcgttc gtgtgcgagg acgactgcac aggggtcgtc
     4321 ctcgcacgcg aa
//
LOCUS       DAOQOD010000230         4286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9136_length_4286_cov_9.385252, whole genome shotgun sequence.
ACCESSION   DAOQOD010000230 DAOQOD010000000
VERSION     DAOQOD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9136_length_4286_cov_9.385252"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2334)
                     /locus_tag="PLZ72_14940"
     CDS             complement(133..2334)
                     /locus_tag="PLZ72_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNA37642.1"
                     /translation="MLGRKRRIQAATNAVRHGYDMALVDAGVVLFQQIAPGIDGHYVS
                     SSIAAALGWDATAFREAGTLRRLVHPDDLARFRSVAPPDDGGDEATSTTIDLRGGTDV
                     SLSSPATLPSTGPAPADAAPDEPVVRFLTAAGDYRPMLVRMVRPRPGEPLRGTLIDVA
                     AGDDLRQRARRLAEVVEVSHHGFLLFELADQDDPTSLVFRAANGAARSLFELDASVLD
                     GGRLDAVFGGPSARLLQSALYDVAHTGESLTAERLSFPEVPDTVVDLRIDRLVDGALG
                     VTVDDVTRTVDVEERLRHQASHDHLTGLPNRGALEDLLSLAAAALGPGEHRAVILVGI
                     DGLDDLNRELGQRNGDRILTELARRLAQDVPGVDSVARIGGGEFVAVSTIHPDRDSAL
                     DRARLVRDALDRPLDVDGDLRHVSCTVGAAIAPEHGLDPGTLLQAADGALRHARSSAD
                     PLAVFDPAEERTTMRRVGLLTELRRGLANQELELRYQPVVDLRTGRVTKVEALLHRQR
                     VAHGAHLSAELLELAERSGLVEPLTRWVIGESARAAERIGRDRDAITVSTNLSMRSLR
                     EDSLLSFLDLLLASGELPAGVLEVELAESDLTEDLDRARTVIEHLRGLGLGVVVDDFG
                     TGFMSIDALKSLPVTGIKIDRGYTTAIASVPTDAESVDWAIGVTHSMGLPVTADGVAD
                     VRSLEMLHDMGCDLAQGLHLSEPVSFEALPGRVAELEQAMLGWVGTSTQPA"
     gene            2367..3398
                     /locus_tag="PLZ72_14945"
     CDS             2367..3398
                     /locus_tag="PLZ72_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNA37643.1"
                     /translation="MAEPPFCSDTGWACDPVWEATGNETIARVIGWLSEKPLQIAGIL
                     ALAYLANRLLRSMIARTGRRMAENADRLGGYMPDGLRTREVDGRAVARAATMSTITRS
                     MATAVVDVVAAAWILAVLGVSLAAIVASAGVLGVALGFGAQNLVRDVLAGWFILVEDR
                     YGVGDTIDAGAPAVGTVERVTLRSTRLRDINGTVWHVANGEILRVGNKSQSWSRAVVD
                     VAVTPDADLERAREILGEVGERMLLDEAWSGLVTSSPNVLGVQRIDPLGITLRVVIDT
                     APGEQFPVEREYRLRVLQAFDAAGISLSAPYAGGPAAAAWPGGPGGPGSPGGTGGIGT
                     GDAGPSSST"
     gene            3542..3721
                     /locus_tag="PLZ72_14950"
     CDS             3542..3721
                     /locus_tag="PLZ72_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37644.1"
                     /translation="MKKVAQPLMALAVGFLILAIWRSPATAAADVTHILGNVGTFLQE
                     VLSKVAEFLGSFGKS"
     gene            3735..4262
                     /locus_tag="PLZ72_14955"
     CDS             3735..4262
                     /locus_tag="PLZ72_14955"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNA37645.1"
                     /translation="MPRRWNPDAIVEDYLLRDEEVKLDATRSFRSWLVDQTVWLLLGV
                     AAFLVLATVGSDVTITLGVGVLAVVGAWLTFDWNRKRFTRYVITDLRVLRITGVLHRR
                     MEFIPWGKITDVSRSESFLQWLTGTATIRIESANERSAFRAMADVSDPEHFYNVLVRM
                     VDLRQGRVKNHHHVR"
BASE COUNT          566 a         1500 c         1611 g          609 t
ORIGIN      
        1 ggatccggcg gggcccgaac ggcaaggccg actaccgctg ggcgaccgac accgcggtcg
       61 ccgccacgtc gtagaccgcc gacgcggtcc ggccgttcgg ccgttcggcg gttcggcgcc
      121 gcggcggccc ggtcaggccg gctgggtgct ggtgccgacc cagccgagca tcgcctgctc
      181 cagctcggcg acgcgcccgg gcagggcctc gaagctgacc ggctcggaca ggtgcaggcc
      241 ctgggccagg tcgcagccca tgtcgtgcag catctccagc gaccgcacgt cggccacgcc
      301 gtccgcggtg accggcaggc ccatcgagtg cgtcacgccg atcgcccagt cgaccgactc
      361 ggcgtcggtg ggtaccgacg cgatcgccgt ggtgtagccg cggtcgatct tgatgccggt
      421 gacgggcagc gacttcagcg cgtcgatcga catgaacccg gttccgaagt cgtcgaccac
      481 cacccccagg ccgagtccgc ggaggtgctc gatgacggtc cgggcccggt ccaggtcttc
      541 ggtcagatcg ctctcggcga gctcgacctc gagcaccccg gcgggcagct cgccggatgc
      601 gagcaacagg tcgaggaacg acagcaggct gtcttcgcgc aggctgcgca tcgagaggtt
      661 ggtgctgacg gtgatggcgt cgcggtcgcg gccgatgcgt tcggcggccc gggccgactc
      721 gccgatcacc caccgggtca gcggctcgac gagcccggac cgctcggcca gctccagcag
      781 ctcggccgac aggtgggcgc cgtgggcgac gcgctggcgg tgcagcagcg cctcgacctt
      841 ggtgacccgg ccggtgcgca ggtcgacgac cggctggtag cgcagctcga gctcctggtt
      901 ggccagacca cggcgcagct cggtgagcag cccgacccgg cgcatggtcg ttcgctcttc
      961 ggcggggtcg aacaccgcca gcgggtcggc ggaggagcgg gcgtggcgca gggctccgtc
     1021 ggcggcctgc agcagggtgc cggggtcgag gccgtgctcg ggggcgatgg cagccccgac
     1081 cgtgcacgag acgtggcgca ggtcgccgtc gacgtcgagc ggacggtcga gcgcgtcgcg
     1141 caccagtcgg gcccggtcca gcgccgagtc ccggtcgggg tggatcgtgc tcaccgcgac
     1201 gaactcgccg ccaccgatgc gggcgacgga gtccacaccc ggcacgtcct gggcgaggcg
     1261 acgggcgagc tcggtcagga tgcggtcgcc gttgcgctgg ccgagctccc ggttgaggtc
     1321 gtcgagcccg tcgatcccga ccaggatcac cgcccggtgc tcgccgggac cgagggcggc
     1381 cgcagccaac gacagcaggt cctcgagggc gccccggttg gggagcccgg tgaggtggtc
     1441 gtggctggcc tggtggcgca gccgctcctc gacgtcgacg gtgcgggtga cgtcgtcgac
     1501 ggtgacgccg agcgcgccgt cgacgagccg gtcgatgcgg aggtcgacga cggtgtcggg
     1561 gacctcgggg aagctcaggc gttcggcggt gagcgactcg ccggtgtggg cgacgtcgta
     1621 gagcgcggac tgcagcagcc gggcgctcgg cccgccgaac acggcgtcga gccggccacc
     1681 gtcgagcacc gaggcgtcga gctcgaacag cgatcgggcg gctccgttgg cggcccggaa
     1741 caccagcgag gtcgggtcgt cctggtccgc cagctcgaac agcaggaacc cgtggtgcga
     1801 cacctcgacg acctcggcca gacgccgggc ccgttggcgc aggtcgtcgc cggcggcgac
     1861 gtcgatcagg gtgccccgga gcggttcgcc gggccggggc cgcaccatgc gcaccagcat
     1921 cggtcggtag tcgccggccg cggtgaggaa cctgacgacc ggttcgtcgg gtgctgcgtc
     1981 ggccggtgcc ggacccgtcg acgggagcgt ggccggcgag ctgagcgaga cgtcggtgcc
     2041 gccgcgcagg tcgatcgtgg tgctggtcgc ctcgtcgccc ccgtcgtcgg gcggggcgac
     2101 cgagcggaac cgcgccaggt cgtcggggtg caccaggcgg cgcagggtcc cggcctcgcg
     2161 gaacgcggtg gcgtcccacc cgagggcggc cgcgatcgaa ctcgacacgt agtgcccgtc
     2221 gatgcccggt gcgatctgct ggaacaggac gactccggcg tcgacgaggg ccatgtcgta
     2281 gccgtggcgg acggcgttgg tggccgcctg gatgcgccgc ttgcgtccga gcatggccgc
     2341 aaatcctact gccctacgat cgggtggtgg ctgaaccccc cttctgctcc gacacgggct
     2401 gggcgtgcga tccggtgtgg gaggccaccg gcaacgagac gatcgcccgc gtgatcgggt
     2461 ggttgtcgga gaagccgctg cagatcgccg ggatcctggc gctcgcctac ctcgccaacc
     2521 ggttgctgcg gtcgatgatc gcccgcaccg gccgacggat ggccgagaac gcggaccgtc
     2581 tgggcggcta catgcccgac gggctgcgga cccgcgaggt cgacggtcgg gcggtcgccc
     2641 gtgccgccac catgtccacc atcacccggt cgatggccac cgccgtggtg gacgtggtcg
     2701 ccgcggcgtg gatcctggcg gttctcggcg tgagcctggc cgcgatcgtg gcgtccgccg
     2761 gcgtgctcgg cgtcgccctg ggctttggtg cccagaacct ggtgcgcgac gtcctcgccg
     2821 gctggttcat cctggtcgag gaccgctacg gcgtcggcga cacgatcgac gccggagcgc
     2881 cggcggtcgg caccgtcgag cgggtcacgc tgcgatcgac gcggttgcgc gacatcaacg
     2941 ggacggtctg gcacgtcgcc aacggcgaga tcctccgggt gggcaacaag agccagagct
     3001 ggtcgcgggc ggtcgtcgac gtggcggtga ccccggacgc cgatctggag cgcgcccggg
     3061 agatcctcgg tgaggtcggc gagcggatgc tgctcgacga ggcgtggtcc ggtctggtca
     3121 cctcgtcgcc gaacgtcctg ggcgtgcagc gcatcgaccc gctggggatc acgttgcggg
     3181 tcgtcatcga caccgcgccc ggcgagcagt tccccgtcga gcgggagtac cggctgaggg
     3241 tgttgcaggc cttcgacgcg gcgggcatct cgttgtctgc gccgtacgcg ggcgggccgg
     3301 ccgccgcggc ctggcccggt ggtccgggag gccctggcag cccgggcgga accggcggca
     3361 tcggaaccgg cgatgccggt ccgtcgtcgt cgacctgagc cgaccatcca ccccggtcga
     3421 cccgccgctc actaccgtcg tcggcggacg gagctccttc cgcgccgcgg cgcggacagg
     3481 gtcaactcgt ggaccccttc cacgcccgac ccgcccgccc acgacccgct cggaggagct
     3541 catgaagaag gtggcgcaac cgctgatggc gctcgctgtc ggcttcctca tcctggccat
     3601 ctggcgcagc cccgcgaccg cggccgccga tgtcacccac atcctcggca acgtcgggac
     3661 cttcctgcag gaggtcctgt cgaaggtggc cgagttcctg ggcagcttcg ggaagtcctg
     3721 aggtctcccg gcccgtgccc cggcgctgga acccggacgc catcgtcgag gactacctgc
     3781 tccgcgacga ggaggtgaag ctcgacgcca cgcgctcgtt caggagctgg ctggtcgacc
     3841 agacggtgtg gctgctgctc ggcgtcgcgg cgttcctggt gctcgccacg gtcggttccg
     3901 acgtcacgat caccctcggc gtcggggtgc tggcggtggt gggcgcgtgg ttgaccttcg
     3961 actggaaccg caagcgcttc acccgctacg tgatcaccga cctgcgggtg ctgcgaatca
     4021 ccggcgtgct gcaccggcgg atggagttca tcccctgggg caagatcacc gacgtcagcc
     4081 gcagtgagtc gttcctgcag tggctcaccg gcaccgcgac gatccgcatc gagtcggcca
     4141 acgagcgctc ggcgttccgg gcgatggccg acgtgtcgga ccccgagcac ttctacaacg
     4201 tgctggtgcg catggtcgac ctccgccagg gtcgggtgaa gaaccaccac cacgtccgct
     4261 gacccagacc ccgccgcccc agaccc
//
LOCUS       DAOQOD010000231         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9179_length_4274_cov_16.082010, whole genome shotgun sequence.
ACCESSION   DAOQOD010000231 DAOQOD010000000
VERSION     DAOQOD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9179_length_4274_cov_16.082010"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLZ72_14960"
     CDS             <1..216
                     /locus_tag="PLZ72_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37646.1"
                     /translation="GRTYAWRTRRSVGPHRSVRRASRSATGERLRPADRDYLVLVALT
                     GRPDGRVRLNKLARDHGWEPAPVSPGT"
     gene            396..965
                     /locus_tag="PLZ72_14965"
     CDS             396..965
                     /locus_tag="PLZ72_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HNA37647.1"
                     /translation="MDSFTMLGLAEDLGISPATLYSHVAGRDEVIALVESRLHGTIRE
                     FATDATEWRGWLADFAALVRRELGSSAATLLNATRDDASMRIDIGEPGLRLLMAAGFS
                     SVEAAYAVWLVVRVAITASSGTDPSFTRFLEPTAELVDAAGDADTFVALRRVHDDLTT
                     SEVHDTFAFDLEIVLDGIAARLERATTGD"
     gene            967..2178
                     /locus_tag="PLZ72_14970"
     CDS             967..2178
                     /locus_tag="PLZ72_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNA37648.1"
                     /translation="MAVTTTRSDIDAFEPAQLGPLTLRNRVIKAATFEGVMPRGAVTD
                     DLIEFHTTVARGGAALSTVAYCAVSMGGRVSSDTMVMTESLIGDLQRLTSAVHAEGAL
                     VSAQLGHAGLVAQSQSKRHPSLAPSGRFSAPAKGWVKGATRRDLDEVVADFERATRVA
                     IEAGFDALEIHLAHNYLLSSFFSPNLNRRRDEFGGSIENRATFPRRVVDAVRRVADGK
                     VAVLAKFNMADGVERGLWLEESLQFAQLLEQDGALDALELTGGSSLLNGMYYFRGDVP
                     LREFAAAQGPIVGLGVRAFGRRILPELPFEEAFFLPFARQFRRELDMPLILLGGINER
                     ATIDGALGEGFEFVAMARALLREPDLVNRMATSPNAVGLCVHCNKCMATIYSRTRCVL
                     APGADQTGSGR"
     gene            2175..2987
                     /locus_tag="PLZ72_14975"
     CDS             2175..2987
                     /locus_tag="PLZ72_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA37649.1"
                     /translation="MKRLSGRVAVVTGAGSGIGRATSLALAERGCSIAVVDVQPDGAE
                     ETADLIRSLGTRASVHVADVRDPKRMGELPSEVEGSLGSCRILVNNAGVTAAGAFADE
                     TVEDLRWIVDINVWGVVHGCRAFLPMLREADEAHIVNMSSMVGLLGLPHNVSYSLTKG
                     AVRAFSEALRGELVTTKVGVTVVFPGAIYTNITAGARGGQAARLAEMGQSRLAPHVMR
                     PPSAVARRIVSAIERDRARVVVGPDAHVVSALTRVLPGRSGLVGRLTSRLDA"
     gene            2996..4177
                     /locus_tag="PLZ72_14980"
     CDS             2996..4177
                     /locus_tag="PLZ72_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNA37650.1"
                     /translation="MDFPAFAVACGEEGSDPLVLHEGRVVRVDQYRASRHLERLDADL
                     ADVAGLGVKVWRYGMPWRLTEPEPGVYDWTLWDRALEACRRHGLDPVIDLCHFGLPDH
                     HGGFCDTTWVDDFARYVDAFLARYPEPRWFTPVNEPGITAMFSSLLGMWNDRLASEDD
                     YFVALGNVALANLEALARIRADRDGWWIGSEGFGCDLADADDEAGIRAATRARDLQQA
                     VWDLHLGLAPRGGAARLTDVVADSIQSRIEALVGVVPPDRIIAGHDIYPVSVTVHGTR
                     AGRPLSIADRVRAYEDEATAWHERYQVPFWVSETSNLGLPVDQGSEWLAALTTSLDTL
                     AASGLPVRGICWYSRGDQYDWDTALMSPIGTVTEVGLFDAQRHPRPVAQHYATLARRH
                     P"
BASE COUNT          656 a         1459 c         1468 g          691 t
ORIGIN      
        1 ggccgaacct acgcgtggcg caccaggcgc tcggtcggtc cacatcggtc agtgcgccgg
       61 gcatcgcgct cggcaactgg tgaacgactg cggccggccg atcgtgacta tctcgtcctg
      121 gtcgccctga ccggtcgccc cgacggccgg gtgcgcctca acaagctcgc gcgcgaccac
      181 ggctgggagc ccgcaccggt ttccccggga acctgacacc gcggtcgcga aacctggtcc
      241 tcgcgtgatc ggggaaggac cagcatttcc gaatcacaat tcggatatag tcgatcacca
      301 tggagaatgg aggtcgtagc gctccgcagg cgcgtcgtgg gcggccgccg aaggtcggtc
      361 gggagagcat cgtggatgcg gcggttcggc tcggcttgga ttccttcacg atgctggggt
      421 tggccgagga cctcgggatc tctccggcga cgctgtactc gcacgtcgcc ggacgcgacg
      481 aggtcatcgc gttggtcgag tcgaggctgc acggcacgat tcgtgagttc gcgaccgatg
      541 cgacggaatg gcgcgggtgg ctggccgact tcgccgcgct ggtgcgtcgc gagttgggct
      601 cgtcggctgc gacgttgctg aacgcgaccc gtgatgacgc gtccatgcgg atcgacatcg
      661 gcgaaccggg cctgcggctc ttgatggcgg ctgggttcag ttcggtggaa gcggcgtatg
      721 ccgtgtggct cgtcgtccgg gtggcgatca ccgcgtcgag cggtaccgat ccgagcttca
      781 cccggttcct ggagccgacc gcggagctgg tcgatgctgc gggagacgcg gacacgttcg
      841 tcgcgctgcg tcgggtccac gatgacctca ccacctcaga ggtccacgac accttcgcgt
      901 tcgatctgga gatcgtgctg gatggcatcg ccgcccggct cgaacgggca acaacaggag
      961 actgacatgg ctgtcacgac cacccggtcc gacatcgacg cgttcgaacc ggcccagctc
     1021 ggccccctga cgctccgcaa tcgggtcatc aaggccgcga cgttcgaagg cgtgatgccc
     1081 cgtggagcgg tcaccgacga cctgatcgag ttccatacga ccgtggcccg aggcggcgca
     1141 gcgctcagca ctgtcgccta ctgcgcggtg tcgatgggcg gtcgggtgag cagcgacacg
     1201 atggtgatga ccgagtccct catcggcgat ctccagcgcc tcacctctgc ggtgcacgcc
     1261 gagggcgcgc tggtgtcggc gcaactcggt cacgcgggcc tcgtcgccca gtctcagtcg
     1321 aagcgccacc cgagcttggc tccctctggc cggttcagcg caccggcgaa ggggtgggtc
     1381 aagggcgcga cacgccgaga cctcgacgag gtcgtggccg acttcgagcg tgcaacccgc
     1441 gtcgcgatcg aagcagggtt cgacgcgctc gagatccacc tcgcccacaa ctatttgctg
     1501 agctcgttct tcagccccaa cctcaaccgg cgccgcgacg agttcggcgg aagcatcgag
     1561 aaccgcgcca cgttcccccg acgggtcgtc gacgcggtgc gccgagtcgc tgacggcaag
     1621 gtggcggtgc tggcgaagtt caacatggcc gacggcgtcg agcgtggcct gtggctcgag
     1681 gagagcctgc agttcgccca gctcctcgaa caggacggcg cgctcgacgc acttgagttg
     1741 accggcggta gctcgctgct gaacggcatg tactacttcc gcggggacgt gcccctccgc
     1801 gagttcgccg ccgcgcaggg ccccatcgtg ggactcggcg tgcgggcgtt cggtcggcgc
     1861 atccttccgg agttgccgtt cgaggaggcg ttcttcctgc cgttcgcccg gcagttccgc
     1921 cgagagctcg acatgccgct gatccttctc ggcggcatca acgaacgggc cacgatcgac
     1981 ggtgctctcg gcgagggatt cgagttcgtc gccatggccc gggcactgct acgggaacca
     2041 gatttggtca acaggatggc gacctcaccg aacgccgtgg ggctgtgtgt tcactgcaac
     2101 aagtgcatgg ccaccatcta cagccggacc cgctgcgtcc tcgcgcccgg agctgaccag
     2161 acgggctcgg gccgttgaag cggctcagcg gacgcgtcgc ggtcgtcacc ggagcgggca
     2221 gcgggatcgg ccgggcgacc agcctggccc tcgccgagcg cgggtgctcg atcgcagtgg
     2281 tcgacgtgca gcccgacggg gccgaggaga ccgctgatct gatccgttcg ctcggcaccc
     2341 gggcgagcgt gcacgtcgcg gatgtgcgtg atccgaagcg catgggcgag ctcccgtccg
     2401 aggtcgaggg ctcgctcggt tcgtgccgga tcttggtgaa caacgccggg gtgaccgctg
     2461 ctggcgcgtt cgcggatgag acggtcgagg atctgcgctg gatcgtcgac atcaacgtgt
     2521 ggggtgtggt gcacggatgc cgtgcgttcc tgccgatgct gcgggaggcc gacgaggctc
     2581 acatcgtcaa catgtcgagc atggtcgggt tgctcggtct tcctcacaac gtgtcgtact
     2641 cgctcaccaa gggggcggtg cgggcgttca gcgaagcgct gcgcggcgag ctggtcacca
     2701 ccaaggtggg ggtcaccgtg gtctttccgg gcgcgatcta caccaacatc accgctggtg
     2761 cgcggggtgg gcaggccgct cggctcgccg agatggggca gtcccgtctg gcgccccacg
     2821 tgatgaggcc gccctccgcg gtcgctcgtc ggatcgtgtc ggcgatcgaa cgcgaccgtg
     2881 cacgtgtcgt cgtcgggccc gacgcccacg tcgtgagtgc gctcacccgg gtgctgcccg
     2941 ggcgatcggg cctcgtcggc cgtctcacga gccgcctcga cgcatgatgt cgaacatgga
     3001 cttccccgcg ttcgccgtcg cctgcggcga ggaaggctcc gacccgctcg tgctacacga
     3061 aggacgggtc gtccgcgtcg accagtaccg agcaagccga catctcgaac ggctcgacgc
     3121 ggacctcgca gatgtcgcgg ggctcggggt gaaggtgtgg cgctacggca tgccctggcg
     3181 gctgaccgaa ccggaacccg gggtctacga ctggacgctg tgggatcgag cgctcgaggc
     3241 gtgccggcgc cacgggctcg acccggtgat cgacctgtgc cacttcggtc tgcccgacca
     3301 ccacggcggg ttctgcgaca ccacgtgggt cgacgatttc gcccgctacg tcgacgcgtt
     3361 cctggcccgc tatccagaac cacggtggtt cacgcccgtc aacgagccgg gcatcaccgc
     3421 catgttctcc tcgctcctcg gcatgtggaa cgaccgcctc gccagcgagg acgactactt
     3481 cgtcgcgctg ggaaacgtcg cgctcgcgaa cctcgaggca ctcgcccgca tccgagccga
     3541 ccgagacggc tggtggatcg ggtccgaagg cttcggctgc gacctcgccg acgccgacga
     3601 cgaggccggc atccgcgccg ccacccgtgc ccgtgacctg cagcaggccg tctgggatct
     3661 gcacctcggt ctcgcacccc ggggcggcgc ggcacgactg accgatgtcg tggccgactc
     3721 gatccagtcg cgcatcgagg ccctcgtcgg agtcgtccca cccgaccgga tcatcgccgg
     3781 ccacgacatc taccccgtca gcgtcaccgt gcacgggaca cgtgccggta ggccgctgtc
     3841 gatcgccgat cgcgtccgcg cctacgagga cgaggcgacc gcctggcatg agcgctacca
     3901 ggtgccgttc tgggtgtccg agacctccaa cctcggcctt ccggtcgacc agggcagcga
     3961 gtggctcgcc gcactcacca catcgctcga cacccttgcg gcgagcggcc tgccggtccg
     4021 ggggatctgc tggtacagcc gaggcgacca gtacgactgg gacaccgcac tgatgtcgcc
     4081 gatcggcacc gtcaccgaag tcggactctt cgacgcccaa cggcacccac gcccggtggc
     4141 gcagcactac gccacgctcg cccgccgaca cccctgaaag agcagcgaag cagcaccgaa
     4201 ccctcacgag gagatggcga cgtgacccac gagaacgagc tgacatccga gatcgacctg
     4261 tgcacgcccg acgg
//
LOCUS       DAOQOD010000232         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9288_length_4244_cov_10.714729, whole genome shotgun sequence.
ACCESSION   DAOQOD010000232 DAOQOD010000000
VERSION     DAOQOD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9288_length_4244_cov_10.714729"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="gnd"
                     /locus_tag="PLZ72_14985"
     CDS             complement(<1..1029)
                     /gene="gnd"
                     /locus_tag="PLZ72_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNA37651.1"
                     /translation="MATDTPMQLGMIGLGRMGANLVRRLMADGHRCVVHDVSADAVAA
                     LEAEGATGAHTLGDFVAALEAPRTVWIMVPAGVVQGTVDELAPLLAAGDTVIDGGNSY
                     YRDDIHRAEALSERGIHYVDCGTSGGVWGLERGYCLMIGGDDEAVERLSPIWETIAPG
                     PMAGAVEPTPGRADGGTAQDGWLHCGPSGAGHFVKMVHNGIEYGMMAALAEGLSIIKH
                     AGVGNAAAGAVADAETTPLRDPWAYRYDIDVAEVAEVWRRGSVISSWLVDLTAAALAQ
                     SPELDEFGGRVSDSGEGRWTVLAAVEAGVPAEVITAALYQRFESRDLGTFTGKVLSAM
                     RSQFGGHAE"
     gene            complement(1083..2714)
                     /gene="pgi"
                     /locus_tag="PLZ72_14990"
     CDS             complement(1083..2714)
                     /gene="pgi"
                     /locus_tag="PLZ72_14990"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNA37652.1"
                     /translation="MPVEPFRERPAHAALVAHHATIAGTHLRDLFAEDPDRGERLVLQ
                     SCGIHADLSKHRATDETFDLLVELADQSGLRDAIDAMFRGDRINVSEDRSVLHIALRR
                     PRDQPLVLDGVDVVAQVNDVLDSMAAFAARVRSGEWVGHTGRPIRNVINIGIGGSDLG
                     PVMAYEALRSYSDRDMTMRFVSNVDATDLVEATVDLDPAETLFIVASKTFTTIETMTN
                     ARSAREWLLAALGPDAEVARHFVAVSTNAEEVAAFGIDTANMFGFWDWVGGRYSMDSA
                     IGLSTMIAVGPERFREMLDGFHAMDEHFRTAPFRQNLPVMMGLLAVWYSEFFDVQTIG
                     VMPYDQYLARFPAYLQQLTMESNGKHVTLDGAAVDYDTGLIYWGEPGTNGQHSFYQLL
                     HQGTRLVPVDLIAFARSRNPLGEHHDVLTSNVFAQSQALAFGRTEAEVRALGTPEAVV
                     PHRVMEGNRPTTVLLSEELTPYRLGTLVALYEHSVFTQATIWGIDAFDQWGVELGKEL
                     AVAILPALTSAEEPDLDTDSSTAALIRTYRALRGR"
     gene            complement(2815..3561)
                     /locus_tag="PLZ72_14995"
     CDS             complement(2815..3561)
                     /locus_tag="PLZ72_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNA37653.1"
                     /translation="MPTSHHTPVHSDAHLAEPRSLLGDLGLGPGTPAGILSDRRLLRR
                     TAMACTDRRATVLDDVELPGCRHRIDHVVIAPHGLWVLSTMGDRGPIELRDVGGFFRS
                     ETRLFVDGRDRTAILDSLAWQQAAVAQRMSVDPTSVTVRSAVVVMGDDDGTSCGTGAD
                     PLDAEEVLDVDGHRVLRLAALGRLITSGAPEGSDALLGQAAIARLAGLLVEDPTAVGV
                     GRATLDLTRRTGRTPDGTDSVLPLSWSTSG"
     gene            complement(3698..>4244)
                     /locus_tag="PLZ72_15000"
     CDS             complement(3698..>4244)
                     /locus_tag="PLZ72_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA37654.1"
                     /translation="AANIDPGWIGVDAVDVIGGALHRPVVVVNDADAAGVAEMRVGAG
                     RGDDGRAVAGVVLMLTLGTGIGSALFVDGTLVPNTELGHLPLRGGDAERYAASSVRKA
                     EGLSMKRYAERLQEYLELVERLLWPDLILIGGGISKRADEFLPLIELRTPLRPARLRN
                     QAGIVGAALLAADDDSRSIAG"
BASE COUNT          602 a         1461 c         1565 g          616 t
ORIGIN      
        1 ctcggcgtgg ccgccgaact gggaccgcat cgcggagagg acctttccgg tgaacgtgcc
       61 caggtcccgg gactcgaagc gctggtagag cgcggcggtg atgacctcgg cgggcacccc
      121 cgcctcgacc gcggcgagca ccgtccaacg gccctcgccc gagtccgaca cccggccccc
      181 gaactcgtcg agctccgggg actgcgccag ggctgcggcg gtcaggtcga ccagccagga
      241 gctgatcacc gacccgcgtc gccacacctc ggccacctcg gcgacgtcga tgtcgtagcg
      301 ataggcccac gggtcgcgca gcggcgtggt ctcggcatcg gcgacggcgc ccgcggcggc
      361 gttgccgacc ccggcgtgct tgatgatgct caggccctcg gccagggccg ccatcatccc
      421 gtactcgatg ccgttgtgga ccatcttcac gaagtggccc gcgccgctgg gcccgcagtg
      481 cagccacccg tcctgggcgg tcccgccgtc ggcccggccc ggcgtgggtt cgacggcgcc
      541 ggccatcggg ccgggagcga tcgtctccca gatcggggac agtcgctcca ccgcctcgtc
      601 gtcgccgccg atcatcaggc agtagccccg ctccagaccc cacacgccgc cgctggtgcc
      661 acagtcgacg tagtggatgc ccctctcgga cagcgcttcg gcccggtgga tgtcgtcgcg
      721 gtagtaggag ttgccgccgt cgatcaccgt gtcgccggcc gcgagcagcg gggcgagctc
      781 gtcgacggtt ccctgcacca cgccggcggg caccatgatc cacaccgtcc gtggcgcttc
      841 gagagcggcg acgaagtcgc ccagggtgtg ggccccggtc gctccctcgg cctcgagggc
      901 cgcgacggcg tctgcgctca cgtcgtgaac gacgcaacgg tggccgtcgg ccatgagccg
      961 gcggacgagg ttcgctccca tccgtccgag tccgatcatg ccgagctgca tcggggtgtc
     1021 ggtcgccatg tcggacctcc gtcgtggtcg gggaccgggt ccgggacccg atccagtctg
     1081 ggtcagcgcc cccgcagggc gcggtaggtg cggatcagcg ccgcggtcga cgagtcggtg
     1141 tccaggtcgg gttcctcggc cgacgtgagc gccggcagga tcgccaccgc cagctccttg
     1201 ccgagctcga cgccccactg gtcgaacgcg tcgatgcccc agatcgtggc ctgggtgaac
     1261 acgctgtgct cgtagagcgc caccagggtg cccaaccggt acggcgtgag ctcctcgctc
     1321 agcaggaccg tcgtcggccg gttgccctcc atcacccggt gcggaacgac ggcctcggga
     1381 gtgccgaggg cccggacctc ggcctcggtg cggccgaagg ccagagcctg gctctgggcg
     1441 aacacgttcg aggtgaggac gtcgtggtgc tcgccgagcg ggttgcggct gcgggcgaag
     1501 gcgatcaggt cgacgggcac cagccgggtc ccctggtgca gcagctggta gaagctgtgc
     1561 tggccgttgg tgcccggctc gccccagtag atgagcccgg tgtcgtagtc gaccgcggca
     1621 ccgtcgaggg tgacgtgctt gccgttggac tccatcgtca gctgctgcag gtaggcgggg
     1681 aaccgggcca ggtactggtc gtagggcatg accccgatgg tctgcacgtc gaagaattcg
     1741 ctgtaccaga ccgccagcag tcccatcatc accggaaggt tctggcggaa cggtgcggtg
     1801 cggaagtgct cgtccatggc gtggaagccg tcgagcatct cgcggaagcg ctcgggtccg
     1861 acggcgatca tcgtcgacag cccgatggcg gagtccatcg agtaccgacc gccgacccag
     1921 tcccagaacc cgaacatgtt ggcggtgtcg atgccgaacg ccgccacctc ctcggcgttg
     1981 gtcgacaccg cgacgaagtg ccgggcgacc tcggcgtcgg ggccgagcgc cgcgagcagc
     2041 cactcccggg ccgagcgggc gttggtcatc gtctcgatgg tcgtgaaggt cttcgaggcg
     2101 acgatgaaca gggtctcggc cgggtcgagg tcgacggtgg cctcgaccag gtcggtggcg
     2161 tcgacgttgg acacgaatcg catcgtcatg tcgcggtcgc tgtagctgcg cagcgcctcg
     2221 taggccatga ccggccccag gtccgagccg ccgatgccga tgttgatcac gttgcggatc
     2281 ggtcggccgg tgtggccgac ccactcgccg gagcggaccc gggccgcgaa cgcggccatg
     2341 gagtcgagga cgtcgttgac ctgggcgacc acgtcgacac cgtcgaggac cagcggctgg
     2401 tcgcgtggtc gacggagggc gatgtgcagg accgagcggt cctcgctgac gttgatccgg
     2461 tcgccacgga acatggcgtc gatggcgtcc cggaggcccg actggtcggc cagctcgacg
     2521 agcaggtcga aggtctcgtc ggtcgcccgg tgcttggaca ggtccgcgtg gatgccgcag
     2581 gactgcaaca ccaggcgctc gccgcggtcg gggtcctcgg cgaacagatc acgcaggtgc
     2641 gttccggcga tggtggcgtg gtgtgcgacc agagcggcgt gggctggacg ttctcggaac
     2701 ggctcgacgg gcacctttcc tccggttcgg tcggggacga gccgagcgta ccggcggatc
     2761 cgggcggcag cgctcaggtc gctgacagga ccctcgcgcc cggtgcaacg ggtctcagcc
     2821 cgacgttgac caggacagcg ggaggacgga gtcggttccg tcgggggtcc ggccggtgcg
     2881 ccgggtgagg tccagggtcg cccggccgac tccgaccgcg gtcggatcct cgaccaggag
     2941 acccgccagg cgggcgatgg ccgcctggcc cagcagagca tcggagccct ccggcgcccc
     3001 gctcgtgatg agccgcccga gtgccgcgag cctgaggacc cggtgcccat cgacgtcgag
     3061 gacctcctcg gcgtcgaggg gatcggctcc ggtaccacac gatgtcccgt cgtcgtcgcc
     3121 catcaccacg acggcgctgc gcacggtgac cgaagtggga tcgacggaca tgcgctgcgc
     3181 gacagcggcc tgctgccagg ccagggagtc caggatcgcg gtgcgatccc gaccgtcgac
     3241 gaacagtctg gtctcggagc ggaagaaccc acccacgtca cggagctcga tcggaccgcg
     3301 gtcgcccatg gtggaaagca cccacagccc gtgtggggcg atgacgacgt ggtcgatccg
     3361 atggcggcag cccgggagct cgacgtcgtc gagaacggtc gcccgccggt ccgtgcacgc
     3421 catcgcggtt cggcggagca ggcgacggtc ggagaggatc cccgccgggg tccccggccc
     3481 caggcccaga tctccgagca gggaccgtgg ttccgcgagg tgggcgtcgg agtggacggg
     3541 ggtgtggtgg gacgtcggca cctctccagg agtaccggag ccccggggag gtgtcgctgg
     3601 gcggatgcac ccggtggcgt gggcactccg ggtcgtagga cccaggcggc tcagggcgat
     3661 cccacggatc ggctcagcgc cggcggtggg cccggggtca gccggcgatc gaccgggagt
     3721 cgtcgtccgc ggcgagcaga gcggcaccca cgatgccggc ctggttgcgc agtcgggccg
     3781 gtcgcagcgg cgtgcgcagt tcgatgaggg gcaggaactc gtcggcccgc ttggagatgc
     3841 ccccgccgat caggatcagg tcgggccaca gcaggcgttc gaccagctcc aggtactcct
     3901 ggagccgttc ggcgtaccgc ttcatggaga ggccctcggc cttgcgaacc gagctggcgg
     3961 cgtatcgctc ggcgtcgcct ccgcgcaacg gcaggtggcc gagctcggtg ttggggacga
     4021 gcgtgccgtc gacgaacagg gcgctgccga tcccggttcc gagggtgagc atgaggacca
     4081 cgccggcgac cgcccgtccg tcgtcgccgc ggcccgctcc gacgcgcatc tccgccaccc
     4141 cggccgcgtc cgcgtcgttg accaccacga ccggtcggtg cagggcgccg ccgatcacgt
     4201 cgacggcatc caccccgatc caacccgggt cgatgttggc cgcg
//
LOCUS       DAOQOD010000233         4233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9324_length_4233_cov_10.776209, whole genome shotgun sequence.
ACCESSION   DAOQOD010000233 DAOQOD010000000
VERSION     DAOQOD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9324_length_4233_cov_10.776209"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..944
                     /gene="modA"
                     /locus_tag="PLZ72_15005"
     CDS             78..944
                     /gene="modA"
                     /locus_tag="PLZ72_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNA37655.1"
                     /translation="MVEHRRSRVAAAPVTLARTAVALLAVFVALAFISGCSSDSDGSA
                     PTTTNASAAASNDSDTSGVSGTIVVSAAASLTEAFTTIRDRFVAANPDAHVTLNFGSS
                     GALSTQILNGAPADVAAFADTAPMNALADADLLAAPPKVFARNRLVIVTEPGNPNGID
                     SLADLATAGTISLCVDTAPCGKFADRILADASVTIPTSNITRGTDVRSTLTAVTDGDA
                     VAGIVYVTDAKTAADRVDTVAIAEAHNVTADYPIATLGATTSPALARAFEAYVLSERG
                     RSVLEDAGFLAP"
     gene            941..1735
                     /locus_tag="PLZ72_15010"
     CDS             941..1735
                     /locus_tag="PLZ72_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37656.1"
                     /translation="MTTRSRHRPPIPVLAAAAVAVAFFALPLLGLIRHASWSTFLADL
                     STPDSLSALRLSLICSLSATGLSVLFGMPLAWVLARVRFPGRALVRGLVLLPLVLPPV
                     VGGVALLTAFSRRGLLGEHLYDWFGIQLTFSTAGVVLAETFVALPFFVITVEAGLRSM
                     DRRYEEAATTLGAGPFTVFRRVTLPLVAPSVIAGAVLSWARALGEFGATITFAGNIVG
                     RTQTLPLAVYQELVTDPDVAVELSLVLLAVSLTVIVSMRDRWLGAS"
     gene            1735..2799
                     /locus_tag="PLZ72_15015"
     CDS             1735..2799
                     /locus_tag="PLZ72_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37657.1"
                     /translation="MLAADIGLTIGSLDLSVELEVDRGEVLALLGPNGAGKTTALRAL
                     AGLEQLDRGSIVLAGTVLDDPTTPTFVPPADRPVGVVFQDYLLFPHLSVLDNVAFGQR
                     CRGVPKRTAEITARRWVDSVGLGSLAGSRPAELSGGQAQRAALARALAADPQLLLLDE
                     PLAALDAGTRSTVRRDLKRHLDQFDGATVLITHDPLDALALADRVVVLEVGRVTQAGT
                     LADVTTRPRTPYVAELLGVNLLRGHGSDHTVVVSDDGVMSDVTVAVGGPVDGETLALI
                     RPNAVSLHRSPPDTSARNQWRCTIDGFDLLGDRVRVRLAGRFDLVAEITPESVSRLGL
                     IEGAEVWASVKATEIATYPA"
     gene            complement(2854..>4233)
                     /locus_tag="PLZ72_15020"
     CDS             complement(2854..>4233)
                     /locus_tag="PLZ72_15020"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNA37658.1"
                     /translation="LDAVATIDALDSGQLSVREVVAAAIERARRIDPQIGAVMFERYG
                     EVLTSVPARRARRGSFEGLPTFIKDMVPVAGLPCTWGAAALEGAPPQKRTRGVARDME
                     AMGMSLVGTSTMPEWGFVPSTEFPERTPTRNPWNPAHTIGGSSGGAAALVAAGVVPVA
                     HAVDGGGSTRIPAACGGLVGLKPSLGRLRRHSDERNLPLSVSVDGVVTRTVRDTARFY
                     AEMERVFRPRSMPPMGEVTGPPSRRLRVGVLGAIPGLADIDEPTRSTLQSTADLLAEL
                     GHHVEETTPPVEPEQFRDDFIFYYRFLVFMATNTAPVVHGSHYDRTRLTKFSQGMAAS
                     FRAEPSRIVGVSRRLRRVRARMAAATRDFDVMLSPTVSTVPPLLGHLGANVDFEPLLE
                     RIAAWMPYTPLANAGGTPAISVPMGVDEGAHLPVGIMLSGDFGSDALLVQLALELEEA
                     RPSDLARIG"
BASE COUNT          597 a         1583 c         1421 g          632 t
ORIGIN      
        1 ccgttctgtt cgttggttca caacgctgac gttgtgaacc aacgaacaga acgggggtgg
       61 gacctagtct ccgggcgatg gtcgagcacc gcaggtcacg ggtcgccgcc gcgccggtga
      121 cgttggcgcg gaccgccgtc gccctcctcg ccgtgttcgt cgctctcgcg ttcatctccg
      181 gctgcagttc cgactccgac ggctccgcac ccaccaccac caatgcctcc gccgcggctt
      241 cgaacgactc cgacacctcc ggggtctccg gaaccatcgt cgtctccgcc gccgcgtccc
      301 tcaccgaggc gttcacgacg atcagggacc ggttcgtggc agccaacccc gacgcgcacg
      361 tgacgctcaa cttcggatcg tccggcgccc tgtcgacgca gatcctgaac ggagccccgg
      421 ccgacgtggc cgcattcgcc gacaccgcgc cgatgaacgc cctcgccgat gccgatctcc
      481 tcgccgcccc gccgaaggtg ttcgccagga accggctggt catcgtcacc gagcccggca
      541 acccgaacgg catcgactcg ctggctgacc tggccaccgc cggcacgatc tcgttgtgcg
      601 tggacaccgc gccctgcggc aagttcgccg accggatcct cgccgatgcc agcgtcacca
      661 tcccgacctc gaacatcaca cgtggcaccg acgtgcgctc cacgctcacc gctgtcaccg
      721 acggggacgc tgtcgccggc atcgtgtacg tcaccgacgc gaagaccgcg gccgaccggg
      781 ttgacaccgt cgcgatcgcc gaggcccaca acgtcaccgc cgactacccg atcgcgacgc
      841 tgggcgcgac caccagcccg gcgctggccc gggcgttcga ggcctacgtc ctgtccgagc
      901 ggggacggtc ggtcctggag gacgccggat tcctcgcgcc gtgaccaccc gctcgagaca
      961 ccgcccgccg attccggtgc tggccgcggc ggcggtggcc gtcgccttct tcgccctgcc
     1021 tctgctcggg ctgatccgac acgcttcatg gtcgaccttc ctcgccgacc tctcaacacc
     1081 ggactcgctc agcgccctgc gcctgtcgct gatctgctcg ctgagcgcca ccggactgtc
     1141 ggtgctgttc ggcatgccgt tggcgtgggt actggcccgg gtccgcttcc ccggacgggc
     1201 cctggtgcgc gggctcgtcc tgctcccgct ggtcctgccg ccggttgtcg gcggcgtggc
     1261 actgctcacc gcgttcagcc gccggggact cctcggcgag cacctctacg actggttcgg
     1321 catccagctc accttctcga ccgccggggt ggtcctggcc gagaccttcg tggcgctgcc
     1381 gttcttcgtg atcaccgtcg aagccggact gcgctcgatg gaccgtcgct acgaggaggc
     1441 cgcaaccacg ctcggggcag gtccgttcac cgtgttccgc cgtgtcacgc tcccgctggt
     1501 cgcaccgagc gtgatcgcag gcgccgtgtt gtcctgggcc cgggcgctcg gggagttcgg
     1561 cgcgacgatc accttcgccg gcaacatcgt cggaaggacc cagacgctgc cgctggcggt
     1621 ctaccaggag ctcgtgaccg acccggacgt ggccgtcgag ctcagcctcg tgctgctggc
     1681 ggtgtcgctc accgtgatcg tgtcgatgcg ggaccgctgg ctcggtgcgt cgtgatgctc
     1741 gcagcggaca tcggcctgac gatcgggtca ctcgatctga gcgttgagct ggaggtcgac
     1801 cgcggcgaag tgctcgccct gctcggaccg aacggggccg gcaagaccac ggcgctgcgc
     1861 gcgctcgccg gcctcgaaca actcgaccgg gggagcatcg tcctggccgg gacggtcctc
     1921 gacgatccga ccacaccgac gttcgtcccc ccagcggacc gccccgtcgg cgtcgtgttc
     1981 caggattatc tgctgttccc ccacctgtcg gtcctcgaca acgtggcgtt cggtcagcgg
     2041 tgtcgagggg tccccaagag aaccgcagag atcacggcgc gacggtgggt cgattccgtc
     2101 ggcctgggat cgttggcggg gtcccgaccc gcagagctgt ccggtggcca ggcccagcgg
     2161 gccgcgctgg cacgggcatt ggctgccgac ccgcagctgc tgctgctcga cgagccgctc
     2221 gccgcgctcg acgccggcac ccgatcgacc gtgcgacggg acctgaagcg gcacctcgac
     2281 cagttcgacg gtgccaccgt cctcatcacc cacgacccgc tcgacgcgct ggccctcgcc
     2341 gaccgcgtcg tcgtgctcga ggtgggacga gtcacccagg ccggcacgtt ggccgacgtc
     2401 accacccggc cccggacgcc gtacgtcgcc gagctgctcg gcgtgaacct gctccgcggt
     2461 cacgggtccg accacaccgt cgtcgtgagc gatgacggtg tgatgagcga cgtgaccgtc
     2521 gcggtcggcg gtccggtcga cggggagacc ctcgccctga tccgaccgaa cgcggtgtcg
     2581 ctgcaccgga gccctccgga caccagcgct cgcaaccagt ggcgatgcac catcgacggg
     2641 ttcgacctgc tcggcgaccg ggtccgcgtc cggctcgccg gtcggttcga tctggtcgcc
     2701 gagatcacac cggagtcggt cagccggctc gggctgatcg agggcgccga ggtctgggcg
     2761 tcggtgaagg cgaccgagat cgccacctac ccggcgtaga gcccgaacgc aggccgactc
     2821 cgacccgata gcggccgccc gaaccgcgac ggctcacccg atccgggcga ggtcggacgg
     2881 tcgggcctcc tcgagctcga gcgcgagctg caccagcagg gcatcggagc cgaagtcccc
     2941 cgacagcatg atcccgacgg gcaggtgagc accctcgtcg acgcccatcg gcacgctgat
     3001 cgccggcgtc cctcccgcgt tcgccagagg ggtgtacggc atccacgccg cgatgcgttc
     3061 gagcagcggt tcgaagtcga cgttcgcacc caggtggccg agcagcggcg ggacggtcga
     3121 caccgtcggg ctcaacatca cgtcgaagtc gcgggtggcc gccgccatcc gggcccgcac
     3181 ccgacggagc cgtcgcgaca cccccacgat gcgggacggc tcggcacgga acgacgccgc
     3241 catgccctgg gagaacttcg tgagtcgggt ccggtcgtag tgggagccgt gcacgacggg
     3301 tgcggtgttg gtggccatga agaccaggaa ccggtagtag aagatgaagt cgtcgcggaa
     3361 ctgctccggt tcgaccggcg gcgtcgtctc ctcgacgtgg tggcccagct ccgcgagcag
     3421 gtcggcggtg gactgcaggg tcgaccgtgt cggttcgtcg atgtcggcca aaccggggat
     3481 ggcgccgagc acgccgaccc tcaacctccg cgacggcggc ccggtgacct cgcccatcgg
     3541 cggcatcgac cgcggtcgga acacccgctc catctcggcg tagaagcggg cggtgtcgcg
     3601 caccgtccgg gtcacgaccc cgtcgaccga caccgacagc ggcaggttcc gctcgtcgga
     3661 atgccgtcgc agacggccga gggacggctt gaggccgacc agccctccgc aggcggcggg
     3721 aatgcgggtc gacccgccgc cgtcgacggc gtgcgcgacc ggcaccacgc cagccgcgac
     3781 cagcgctgcg gcgccaccgg aggagccgcc gatcgtgtgg gccgggttcc acgggttgcg
     3841 cgtcggcgtc cgctccggga actcggtcga aggcacgaat ccccactcgg gcatggtcga
     3901 cgtccccacc agcgacatcc ccatcgcctc catgtccctg gcgacgcctc gggtccgctt
     3961 ctgcggggga gcaccttcga gggccgctgc gccccaggtg cacggcagac ccgcgaccgg
     4021 gaccatgtcc ttgatgaacg tcggaaggcc ctcgaacgag ccgcgtcgag cgcgccgcgc
     4081 cggcacggag gtcagcacct cgccgtagcg ctcgaacatg accgccccga tctgcgggtc
     4141 gatccgccgg gcccgctcga tcgccgcggc gaccacctcc cggaccgaca gctggcccga
     4201 gtcgagcgcg tcgatcgtcg ccaccgcgtc gag
//
LOCUS       DAOQOD010000234         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9382_length_4220_cov_10.311405, whole genome shotgun sequence.
ACCESSION   DAOQOD010000234 DAOQOD010000000
VERSION     DAOQOD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_4220_cov_10.311405"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PLZ72_15025"
     CDS             <1..1469
                     /locus_tag="PLZ72_15025"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNA37659.1"
                     /translation="WWRVGYKFGGGGGVGGPVAGWADAERAALSSPERPPPRVTPDEL
                     LSIADDVVARARPDEELEAVVSWSHDTEIRAADGEVEHFVESDSAGLGVRIVRDGRTG
                     MAWVGHLGDREALDECIAEARDNAAFGTPDPDAGLARPDGVAPVTLDLFDPRLAEVAT
                     DAKIELAVALERAILDADPRMVGLEASDYADSVAAGAIATTTGIRVAGADTSAYLGAW
                     ALAEDDSGTTTGFGFSIGRSTEDLRLEPAAADAVNRCLSMLGAAKAPSERLTVVFDPY
                     VTSQFLGLVAELLSGEAVLRGRSAFAGRVGESVASPLVTLIDDPTDPLAPTADDTDGE
                     GLATRTVPLISGGVLGGYLHNAYTARASGVTSTGSAQRGGHKGVPGVGPRVAKLTGGS
                     GGLADVLARVGDGLLVTELSGLHSGVNPVSGDLSVGVEGRRIRNGELAEPVREVTIGS
                     TLPRMLHDVVAVGADTTYFPWDATGVSLAVSDVTMSGT"
     gene            1466..2818
                     /locus_tag="PLZ72_15030"
     CDS             1466..2818
                     /locus_tag="PLZ72_15030"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNA37660.1"
                     /translation="MSGGDADLVALARSLVDSATAGEQIEVAVGRSTSTSVKVFGGEV
                     ESFTSASTDGAGVRVIRDGRQGFAHCGSLEPDVLAETLAEARDNCRFAEPDEFNGLAE
                     PDGVEPVAQDGWSDAVVALGNDGKVGIALELERLTTGIDPRVTSARTTAYHDGWGESA
                     VVSSNGIEVFQRSGSCSASTSPMARADGETQMGWAVDASRDPSALDLDQVAADAVQRA
                     VRLLGATKPPSSRRTILLEPRLAITLVSLVAGMLSGDAVTKGRSPFADRVGESVASPL
                     LTFVDDPTRSESIAADEYDGEGLACRRNDLIVGGVLQGFLHDSYTGRRSGESSTASAV
                     RGVRSLPGVGAQLLVMTPGERSFEELVAGIDRGLYVTSFAGLHSGVNPVSGDFSVGAD
                     GLLIDGGQLGAPVREVTIASTLQRLLLDVVEVGGDFEWTPGGSGACSLVIDDVAVSGS
                     "
     gene            complement(2892..>4220)
                     /locus_tag="PLZ72_15035"
     CDS             complement(2892..>4220)
                     /locus_tag="PLZ72_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNA37661.1"
                     /translation="GGFPGGADMSFGDMGAFLQGLSSMLAGGSGSGGSWDAAAQLAGS
                     IATQGRSEPNLDPADRIAVEQLARVAELQVQGIVTVGADTPVRLEPLNRTQWARRFLD
                     DERPLLERLSGSLGRALQAQLGELEKEADDDPLAAMGGLGSADALIRQVMAMMGPMLL
                     GMMAGSTAGHLAARAFGHYELPLPRPADGPITMVLSNVDEFADEWSLPRDSVRLWVVL
                     SDVTHHRVLGIPHVRAHLDELLSAYSSGFNDDVGDMERRMEELGIPDLLGTGEPDMAA
                     LQRLAGDPDILLGAMQSDAQRELMPRIETLVATIEGYVDHVLDRLGRRLLADYDRVTE
                     AMRRRRVEFGPERRFVERLFGLELRQATFDRGAAFVEGLVDRAGVEVLDELWGDVERL
                     PTPAELEAPGLWLARVGVDVGDGVDDLPELDEPFEVPDFLDLGDDGTPES"
BASE COUNT          544 a         1509 c         1586 g          581 t
ORIGIN      
        1 tgtggtggcg tgtgggctac aagttcggcg ggggcggcgg ggtcggtggc cccgtggcag
       61 gctgggccga tgccgagcgt gctgccctct cctcgcctga acgacccccg ccccgcgtga
      121 cccccgacga actgctgtcc atcgccgatg acgtcgtggc ccgggcgcgg cccgacgagg
      181 agctcgaagc cgtcgtctcc tggagccacg acaccgagat ccgggccgcg gacggcgagg
      241 tggagcactt cgtcgagtcc gacagcgccg ggctcggcgt gcgcatcgtc cgcgacggtc
      301 ggaccggcat ggcgtgggtc ggtcacctcg gagaccgcga ggccctcgac gagtgcatcg
      361 ccgaggcccg cgacaacgcc gcgttcggca cgccggaccc cgacgccggc ctcgcccgtc
      421 ccgacggcgt cgccccggtg acgctcgacc tgttcgaccc ccggctggcc gaagtggcca
      481 ccgacgccaa gatcgagctg gccgtcgcgc tcgaacgggc gatcctcgac gccgatcccc
      541 gcatggtcgg actggaggcg tcggactacg cggactccgt cgccgccggg gcgatcgcca
      601 ccaccaccgg gatccgggtc gccggagccg acacctcggc ctacctcggc gcatgggcgt
      661 tggccgagga cgactcgggc accacgacgg ggttcggctt ctcgatcggg cgcagcaccg
      721 aggacctccg gctcgagccc gccgccgcgg acgccgtgaa ccgctgcctg tcgatgctgg
      781 gtgcggccaa ggccccgagc gaacggctca ccgtcgtgtt cgacccgtac gtgacgtcgc
      841 agttcctggg tctggtcgcc gagctgctct cgggcgaggc ggtcctgcga ggacggtccg
      901 cgttcgccgg ccgggtgggg gagtcggtgg cctcgccgct ggtcaccctc atcgacgacc
      961 cgaccgatcc gctcgccccc accgcggacg acaccgacgg cgagggcctg gccacccgga
     1021 cggtccccct gatctcgggc ggcgtgctcg gcggctacct gcacaacgcc tacacggccc
     1081 gggcgtcggg cgtcaccagc accggatccg cccaacgcgg cggccacaag ggcgtgccgg
     1141 gggtgggtcc ccgcgtggcc aagctcaccg gcggctccgg cgggctcgcc gacgtcctcg
     1201 cccgggtcgg cgacgggctg ctggtgaccg agctgtcggg gctgcactcc ggggtgaacc
     1261 cggtgtcggg agacctgtcg gtcggcgtcg aaggtcgtcg gatccgcaac ggcgagctgg
     1321 ccgaaccggt gcgcgaggtg acgatcggct ccaccctgcc ccgcatgctg cacgacgtcg
     1381 tcgcggtcgg cgccgacacg acctacttcc cgtgggacgc gaccggggtc tcgttggcgg
     1441 tgtccgacgt gaccatgtcg ggcacgtgag cggcggcgac gccgatctgg tcgccctggc
     1501 ccgatcgctg gtcgacagcg cgacggccgg cgagcagatc gaggtcgcgg tcggtcggtc
     1561 gacgtcgacg tcggtgaagg tgttcggcgg cgaggtcgag tccttcacct ccgccagcac
     1621 cgatggcgcc ggcgtccggg tcatccgcga cggccgtcag gggttcgcgc actgcgggtc
     1681 gctcgagccc gacgtgctgg ccgagaccct ggccgaggcc cgcgacaact gccggttcgc
     1741 ggagcccgac gagttcaacg ggctggccga acccgacggc gtggagccgg tggcccagga
     1801 cggctggtcc gacgcggtgg tcgccctcgg caacgacggc aaggtcggaa tcgccctgga
     1861 gctggaacgc ctgacgaccg ggatcgaccc ccgggtcacc tcggcgcgca ccaccgccta
     1921 ccacgacggc tggggcgagt ccgcggtcgt gtcgagcaac gggatcgagg tgttccagcg
     1981 cagcgggtcg tgctcggcgt ccacctcgcc catggcccgc gccgacggcg agacccagat
     2041 gggctgggcc gtcgacgcgt cgcgggaccc ctccgcgctc gacctggacc aggtcgcggc
     2101 ggacgcggtg cagcgggcgg tgcgactgct cggcgcgacc aagccgccgt cgtcgcggcg
     2161 gacgatcctg ctcgagcccc gcctcgccat cacgctggtg tcgctggtgg cagggatgct
     2221 gagcggagac gccgtcacca agggccgctc gccgttcgcg gaccgggtcg gcgagtcggt
     2281 cgcgtcaccg ctgctcacct tcgtcgacga cccgacccgc tcggagtcga tcgcggccga
     2341 cgagtacgac ggcgagggcc tggcctgccg gcgcaacgac ctgatcgtgg gcggcgtgct
     2401 gcaggggttc ctccacgaca gctacaccgg ccgtcgctcc ggggagtcat ccaccgcgtc
     2461 cgcggtccgc ggcgtgcggt ccctgcccgg ggtcggggcg cagcttctgg tgatgacgcc
     2521 cggcgagcgc agcttcgagg agctggtggc gggaatcgac cgggggctct acgtcacgtc
     2581 cttcgccggc ctgcactcgg gggtgaaccc ggtctcgggc gacttctcgg tcggcgccga
     2641 cggactgctc atcgacggcg ggcagctcgg agcgccggtg cgcgaggtca ccatcgcatc
     2701 caccctccag cgactgctgc tcgacgtcgt cgaagtcggc ggggacttcg agtggacgcc
     2761 gggcggttcc ggggcctgct cgctggtgat cgacgacgtc gccgtcagcg ggagctgacc
     2821 ggtccgactg ccggggtccc ggcctcggaa cgggatatgg ggtccgcggt gggcgggttc
     2881 gctgccggcg gtcagctctc gggggtgccg tcgtcgccca ggtcgaggaa gtccggtacc
     2941 tcgaacggct cgtcgagctc gggcaggtcg tcgaccccgt cgccgacgtc cacgccgaca
     3001 cgggccagcc acagacccgg cgcctcgagc tcggccgggg tggggaggcg ttcgacgtcg
     3061 ccccacagct cgtcgaggac ctcgacgccg gcccggtcga ccagaccctc gacgaaggcg
     3121 gcgccccggt cgaaggtcgc ctggcgcagc tccaggccga acagccgctc cacgaaccgg
     3181 cgctccgggc cgaactcgac ccggcgtcgg cgcatggctt cggtgacgcg gtcgtagtcg
     3241 gcgagcagcc ggcgtccgag ccggtcgagc acgtggtcga cgtagccctc gatggtggcg
     3301 accagcgtct cgattcgtgg catcagctcg cgctgggcgt cggactgcat ggcgccgagg
     3361 aggatgtcgg ggtcgccggc caggcgttgc agagcggcca tgtcgggttc gccggtgccg
     3421 agcaggtcgg ggatcccgag ctcctccatg cgccgctcca tgtcgccgac gtcgtcgttg
     3481 aaccccgagc tgtaggcgct gagcagctcg tcgaggtgcg ctcgaacgtg cgggatgccg
     3541 agcacccggt ggtgggtgac gtcggagagg acgacccaca ggcgcaccga gtcccggggc
     3601 aggctccact cgtcggcgaa ctcgtcgacg ttggacagga ccatggtgat cgggccgtcg
     3661 gcgggccggg gcagcggcag ctcgtagtgg ccgaaggcgc gtgcggccag gtgcccggcg
     3721 gtgctgccgg ccatcatgcc gagcagcatc gggcccatca tcgccatcac ctggcggatc
     3781 agcgcgtcgg ccgagccgag tccgcccatc gccgccaacg ggtcgtcgtc ggcctccttc
     3841 tcgagctcgc cgagctgagc ctgaagggcc cggccgaggg agccggagag gcgttcgagc
     3901 agcgggcgtt cgtcgtcgag gaatcggcgg gcccactggg tccggttcag cggctccagc
     3961 cggacggggg tgtcggcgcc gacggtgacg atcccctgca cctgcagttc ggcgacccgg
     4021 gccagctgct cgacggcgat gcggtcggcc gggtcgaggt tcggttcgga gcgtccctgg
     4081 gtggcgatcg atcccgccag ttgagcggcc gcgtcccagc tgcccccgga gccggaaccg
     4141 ccggcgagca tcgacgacag cccctggagg aacgcgccca tgtcgccgaa ggacatgtcg
     4201 gccccgcccg gaaagccgcc
//
LOCUS       DAOQOD010000235         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9518_length_4183_cov_10.982074, whole genome shotgun sequence.
ACCESSION   DAOQOD010000235 DAOQOD010000000
VERSION     DAOQOD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9518_length_4183_cov_10.982074"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLZ72_15040"
     CDS             <1..844
                     /locus_tag="PLZ72_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNA37662.1"
                     /translation="AALSIAAADLGVRPVVDISAPARSAQALRVAAVVPTLVAGLHRA
                     SLGLAPVEPDPDLNHAGAYLQMVTGERPSPAAERALEQYLILTIDHGFNSSTFTARVV
                     ASTGADLVDAVTTALGALAGPLHGGAPGRALDALDAIGDPDRAREWATAELAAGRRIM
                     GFGHAVYRSRDPRSDLLRAIALGLVDQLGGDLVERAVAVEGHILDALREAKPERALPS
                     NVEYYAGVVMAAAGLPRSLFTPTFAVSRSVGWCAHAVEQAAAAKLIRPSARYVGAPPP
                     ASAA"
     gene            1090..2010
                     /locus_tag="PLZ72_15045"
     CDS             1090..2010
                     /locus_tag="PLZ72_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNA37663.1"
                     /translation="MGSAIEVAGLTKRYDGRTVVDSISFEVRNGECFGILGPNGAGKT
                     TTLEMVEGLRRPDGGTVSLLGTSPWPRDTSLLRRIGVQLQATAFIEHLTSIEQLRTVN
                     DLYGVPATRAEEVLEQVGLIDAADRDVDALSGGQRQRLSIACALVHRPEVLFLDEPSA
                     GLDPGARRNLWDVVRTVRRDDTTVVLTTHHMDEAEQLCDRVAIMDRGEILALDTPAEL
                     VRRLDAPTRLMLPPGSLSDDQAHGVDGVESVERDAGAVTLVTRRAADSIQALAALGTL
                     DGLQVRSGTLEDVFISLTGRQLAEPTEEAP"
     gene            2007..3047
                     /locus_tag="PLZ72_15050"
     CDS             2007..3047
                     /locus_tag="PLZ72_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNA37664.1"
                     /translation="MRAWWSLSRAMFKGLLADKTALFFYFLFPLMFLVLFGLLLGSPG
                     AGKVTVGVAGKGPLIEAVPSEVISLQRFGSFDDAVAAVRDGDIPAAVRQDGDVVELRY
                     SATDQVSAGTVQGIVQSVVGRANLELAGTPPRFTVDSAQVEDESFEPIQYLTSGLLAW
                     AVAMSAAFGAAMNLVTWRRNEVLRRLRMSPVSPAAVVGARIGVSLVVAVAQAAVFIAV
                     AVTPAFGLQLRGSWWLALPLLIAGTLAFLAVGLLIGSIARTEEAASAAVNLIVLPMAF
                     LSGVFFPIDDMPGWVQGVASFMPMRHLSTGLLDVLVRDATVGAILVPLAVLLGFAAVV
                     TLVATRVFTWDT"
     gene            complement(3173..4093)
                     /locus_tag="PLZ72_15055"
     CDS             complement(3173..4093)
                     /locus_tag="PLZ72_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNA37665.1"
                     /translation="MRRPADDRPPDGAVLAFLRAHHGVDVLDLVALGGGFWSASYAYR
                     VGSDELVLRLADTPDGFRADESAQAYGSASMPVPEVLAIGQGLDRWFAISRRHHGRFL
                     EDVSPDEADAVAPAVIGLLRDLSAVRSTRGTPPPWRAWLLDGITDRPGLPTSGWRSAV
                     ADRPRADRAFREADARIRSLLDACPNDRRLVHGDLLHGNVLMSPAGDAVTAVFSWKCA
                     TWGDPLYDLAWCTFWGRWHDGIGAMDLWGRVVPSLTGSEAVDVAQRHHVYEVQIAASH
                     LGWYATLGDEDNLTWTVRQIGDLLEQGPRR"
BASE COUNT          583 a         1572 c         1427 g          601 t
ORIGIN      
        1 cgccgcgctg tcgatcgccg cggccgacct cggcgtccgc ccggtggtcg acatctccgc
       61 gccggcgcgg tccgcccagg cgctcagggt tgccgcagtc gtgcccaccc tggtcgcggg
      121 cctgcaccgg gccagcctgg gcctggcacc ggtggagccc gatccggacc tcaatcacgc
      181 cggcgcctac ctccagatgg tgaccggcga gcggccgtca ccggccgcgg agcgcgcgct
      241 cgagcagtac ctgatcctga ccatcgacca cgggttcaac tcctcgacgt tcacggcgcg
      301 ggtggtggcc tcgaccggcg ccgatctcgt cgacgccgtc accacggcgc tcggggcgct
      361 ggccggccca ctgcacggcg gggctccggg ccgggccctc gacgccctcg acgcgatcgg
      421 tgacccggac cgggcgcggg agtgggccac ggccgagctc gccgccggac gacgcatcat
      481 gggcttcggc cacgccgtgt accgctcccg cgatccccgc tcggacctgc tccgtgcgat
      541 cgccctgggc ctggtcgacc agttgggtgg cgacctggtg gagcgggcgg tcgcggtcga
      601 gggccacatc ctcgacgcgc tgcgcgaggc caagccggaa cgggcgctgc cgagcaacgt
      661 cgagtactac gccggggtgg tgatggcggc ggccgggctg ccccgctcgt tgttcacgcc
      721 gaccttcgcc gtcagccgga gcgtcggctg gtgcgcccac gccgtcgagc aggccgcggc
      781 ggccaagctc atccgcccga gcgcccgcta cgtcggcgct cccccaccgg cgtcggccgc
      841 atgaccccgc cgaacttgta gcccccacgc caccccagcg accaccgact acaacaacgc
      901 cagccgccgc cgaacttgta gcccccacgc caccccagcg accgccaact acaacatcgc
      961 cagccgccgc cgagcttgta gcccacacgc caccccagcg accgtcagct acaagttcgc
     1021 cggataccgc ggacaacgcg caccgcgccg gggtccctga gcactggcga acccgttagc
     1081 gttcggcccg tgggctcagc gatcgaggtg gccggactca ccaagcgcta cgacggccgg
     1141 accgtcgtcg actcgatcag cttcgaggtc cgcaacggcg agtgcttcgg gatcctcggc
     1201 cccaacggcg ccggcaagac caccacgctg gagatggtcg aggggctccg acggcccgac
     1261 ggcggcacgg tgtcgctcct cgggaccagc ccgtggcccc gggacacctc cctgctgcgt
     1321 cgaatcggcg tgcagctcca ggccaccgcg ttcatcgagc acctcacctc gatcgagcag
     1381 ctgcgaaccg tcaacgatct gtacggggta ccggccaccc gcgccgagga ggtgctcgag
     1441 caggtcggcc tcatcgacgc ggccgaccgc gacgtcgacg ccctctccgg tgggcagcgc
     1501 cagcgcctgt cgatcgcctg cgccctggtg caccgacccg aggtcctgtt cctcgacgaa
     1561 ccctctgccg gactcgaccc gggagctcgg cgcaacctgt gggatgtggt ccgcaccgtc
     1621 cgccgcgacg acacgaccgt cgtgctgacc acgcaccaca tggacgaggc cgagcagctc
     1681 tgcgaccggg tggcgatcat ggaccggggc gagatcctgg ccctcgacac gcccgccgag
     1741 ctcgtccgcc ggctcgacgc accgacgcgg ctgatgctgc caccgggttc gctctccgac
     1801 gatcaggccc atggtgtcga cggggtcgag tcggtcgagc gtgacgccgg cgcggtgacc
     1861 ctggtgacgc gccgggccgc ggattcgatc caggcgctcg ccgccctcgg cacgctcgac
     1921 gggttgcagg tccgatccgg aaccctcgag gacgtgttca tctccctgac cggtcgacag
     1981 ttggccgagc cgaccgagga ggcaccgtga gggcctggtg gtcgctgtcg cgggcgatgt
     2041 tcaagggcct gctcgccgac aagaccgccc tgttcttcta cttcctgttc ccgctcatgt
     2101 tcctggtgct gttcgggctc ctgctcggca gtcccggcgc cggcaaggtc accgtcggcg
     2161 tcgccggcaa gggcccgctg atcgaggcgg tgccgtccga ggtgatcagc cttcagcgct
     2221 tcggctcctt cgacgatgcc gtcgcagcgg tgcgcgacgg cgacatcccc gccgcggtcc
     2281 gccaggacgg cgacgtcgtc gagctgcgct actcggccac cgatcaggtc tcggcgggga
     2341 cggtgcaggg gatcgtccag tcggtggtcg gccgggcgaa cctcgagctc gccggaaccc
     2401 cgccccgctt caccgtcgac tcggcgcagg tcgaggacga gtccttcgaa ccgatccagt
     2461 acctgacctc tggcctcctg gcgtgggcgg tggccatgtc cgccgccttc ggcgcggcga
     2521 tgaacctggt cacctggcga cgcaacgagg tgctgcgccg tctgcgcatg tcgccggtgt
     2581 cgcccgcagc ggtggtcggg gcccggatcg gcgtgagcct ggtcgtggcg gtggcccagg
     2641 ccgcagtgtt catcgccgtg gcggtcaccc cggcgttcgg actgcagctc cggggttcgt
     2701 ggtggctggc cctgccactg ctgatcgccg gcacgctcgc tttcctggcc gtcggcctgt
     2761 tgatcggctc catcgcccgc accgaggagg cggcttcggc cgcggtgaac ctgatcgtgc
     2821 tgccgatggc gttcctctcc ggtgtgttct tcccgatcga cgacatgccg ggctgggtgc
     2881 agggggtggc gtcgttcatg ccgatgcggc acctgagcac gggcctgctc gacgtgctgg
     2941 tccgcgacgc gaccgtcgga gcgatcctcg tcccgctcgc ggtcctcctc ggcttcgccg
     3001 cggtcgtgac gctggtggcg acacgggtct tcacctggga cacctgacgc cgacccccgc
     3061 cgaacttgta gcccacgcgc caccccagcg accgccagct acaagatcgc cggcgtccac
     3121 cgaacttgta gcccacgcgc caccccagcg accgcccgct acaagatcgc cttcaccgtc
     3181 ggggtccctg ctccagcagg tcgccgatct ggcgaaccgt ccaggtcagg ttgtcctcgt
     3241 cgcccagagt ggcgtaccag ccgaggtggc tcgccgcgat ctgcacctcg tagacgtggt
     3301 gacgctgagc gacgtcgacc gcttccgaac ccgtcagcga gggcacgacc cggccccaca
     3361 ggtccatcgc tccgatgccg tcgtgccaac ggccccagaa cgtgcaccag gcgaggtcgt
     3421 agaggggatc cccccaggtc gcgcacttcc aggagaagac cgcggtgacc gcatcgccag
     3481 cgggcgacat caggacgttg ccgtggagca ggtcaccgtg gacgagccga cgatcgttcg
     3541 gacaggcgtc gagcagggat cggatccgag cgtccgcctc gcggaacgcc cgatccgccc
     3601 gcgggcggtc ggcgacggct gaccgccagc ccgacgtcgg gagtcccggc cgatcggtga
     3661 tcccgtcgag gagccacgcc cgccacggcg gaggagtgcc cctggtgctg cgcaccgccg
     3721 agaggtcccg gagcagtccg atcacggccg gggccacggc gtcggcctcg tcggggctca
     3781 cgtcctcgag gaagcgaccg tggtgccgcc gggagatggc gaaccaccgg tcgagcccct
     3841 ggccgatggc cagcacctcg gggaccggca ttgacgccga gccgtacgcc tgggccgact
     3901 cgtcagcccg gaacccgtcg ggcgtatcgg caagccgcag cacgagctcg tcgctgccga
     3961 ccctgtacgc gtacgaggca gaccagaacc caccaccgag cgcgacgaga tccaggacat
     4021 cgacgccgtg gtgtgcacgc aggaaggcga gtacggcccc gtcgggaggt cggtcgtcgg
     4081 ccggtcgtct cacgatcgca gactggtgct cgctcggccg actccgcaac gagattgcgg
     4141 ccaacaggcc ctcggctcga cgacgcgtcc gatcccgagg ccg
//
LOCUS       DAOQOD010000236         4099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_9844_length_4099_cov_8.424580, whole genome shotgun sequence.
ACCESSION   DAOQOD010000236 DAOQOD010000000
VERSION     DAOQOD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9844_length_4099_cov_8.424580"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLZ72_15060"
     CDS             complement(<1..684)
                     /locus_tag="PLZ72_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37666.1"
                     /translation="MTTRHRGDATPGVLAAAEVGLLAVGLATVLGFSRLFLGTRYLGP
                     LALAVTASWALALITRRLGWGVGRSALVSTVSALLVLTWTFAAHTTWAGLPTPTTADS
                     VLTDVQRAFRGFSRMVAPVPATTGFLVLLAIVMWAFTFFADTAAFRFRGPVQAVIPYA
                     SAFIATGVLARDSGRVGAAMAFLSGLAIYAVTQRALRASERRWIPGQARSGTTAVAAG
                     AAVAVAVAVV"
     gene            complement(681..1931)
                     /locus_tag="PLZ72_15065"
     CDS             complement(681..1931)
                     /locus_tag="PLZ72_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNA37667.1"
                     /translation="MRPTRAGVAVAATIPLLVAGAWLLGQPELAAAAAAATAVLVVAA
                     VTVRSRRPTLELGRTLHPARVALGEPCEVHLSIRNVGRRRTPVLALDDDAGVFGRARL
                     GIAPVPVGATRDASYSFPTHRRGLHPVGPLTVDVEDAFGLVRSRRVMDDARTLIVLPR
                     TWTLPSLPHAPGDEPEHGTRALSSTSTVDEEFASLRPYSVGDDIRRIHWRTTARIGEP
                     VVRQYDQPWQRRSTILLDVRTLAATGPGNDSTPGKDSTPGNDSTPGSDPAFERAVSAA
                     ASLVVLAATDREMVRLVLTDGSDSGFVPAAESVEELLDRLAATRPDPRASLAGALAGL
                     GARPSGRLVTCTRHLEPGERRGWARHTVGYGMRVLLSTGMPSPGETSDRTDSTLEVTW
                     VDDGPLDRAWTAAVAPATPRERVR"
     gene            complement(1942..2916)
                     /locus_tag="PLZ72_15070"
     CDS             complement(1942..2916)
                     /locus_tag="PLZ72_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNA37668.1"
                     /translation="MTTMDPTTTPAGPGRSFSAMFDSICSNIAVAVRGKDDVIRLTVT
                     CLVAGGHLLIEDVPGVGKTTIAKALARSVDGRFGRVQFTPDLLPSDVLGSSVWNQSSG
                     AFEFRAGPVFHHLVLSDEINRASPKTQSALLEAMAEEQVTVDGTTYPLPRPFMVVATQ
                     NPLEHQGTFPLPESQLDRFLMRLSVGYPDRVAETALLTETDHDDRLAGLGPVVSTEDV
                     LAMGAAAAGVFVSDALASYLVELADRSRRSGAFALGMSPRAALGLLGAARVHAAAQGR
                     TFVSPDDIKDLAVPVLAHRVLLATESQLRGVTAADAVADLLRTTPVPG"
     gene            3004..3897
                     /locus_tag="PLZ72_15075"
     CDS             3004..3897
                     /locus_tag="PLZ72_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNA37669.1"
                     /translation="MAAVLALDVGGTKLGAAVVADDGRVVAEGRRPTVATDADELFEL
                     VTDLLAGSLDAAAERGVPRDDLRAVGVGCGGPMTAGGEEVSPLNIGVWRGFPLRRRLA
                     EWSGLPTFVDNDAKALALGEGWVGAARGVSDYLAMVVSTGVGGGLVLDGRLLDGGAGN
                     AGHIGHVIVDPLGRRCGCGATGCLEAQVSGTAIAATTGRPAAEADPEMRIRVGRLVGR
                     AVASVANLLDLRLAVVAGSVALGFGGVFFDAANAELDRCARLEFSRGARAVPSGCGAH
                     GPLIGAAAVGWRGIGHDVGVR"
     gene            3894..>4099
                     /locus_tag="PLZ72_15080"
     CDS             3894..>4099
                     /locus_tag="PLZ72_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37670.1"
                     /translation="MSRGGRSGIGVPPRAVLAVLRRPDLWWTGVRQALVLAPDGWWRR
                     PPHLPLPDPAYLRFRMVTAHGGDGS"
BASE COUNT          512 a         1416 c         1648 g          523 t
ORIGIN      
        1 gaccaccgcg acggcgaccg cgactgcggc gccggcggcg acggcggtgg tcccgctccg
       61 ggcctgtccc ggtatccacc gccgttcgct ggcgcgcagg gccctctggg tgacggcgta
      121 gatggccagc cccgacagga acgccatcgc ggcgcccacc cgtccgctgt cgcgggcgag
      181 caccccggtg gcgatgaacg ccgaggcgta cgggatgacg gcctggaccg gaccccggaa
      241 gcggaacgcg gcggtgtcgg cgaagaaggt gaaggcccac atcacgatcg cgagcaggac
      301 caggaacccg gtggtggccg gcacgggcgc gaccatgcgg gagaagccgc ggaacgcccg
      361 ctgcacgtcg gtcaggaccg agtccgccgt ggtcggcgtc ggaagcccgg cccaggtggt
      421 gtgggccgcg aacgtccagg tcagcaccaa gagggcgctg acggtcgaga cgagtgcgga
      481 tcgaccgacg ccccagccga ggcggcgggt gatcagcgcc agggcccagc tggcggtgac
      541 ggccagggcg agcgggccga ggtaccgggt ccccaggaac aggcgcgaga agccgagcac
      601 ggtcgccagg ccgaccgcca ggagcccgac ctcggcggcg gccaggacgc ccggcgtggc
      661 gtcgccgcgg tgccgggtgg tcaccggacc cgctccctgg gtgtcgctgg cgcgacggcc
      721 gccgtccacg cccggtcgag ggggccgtcg tcgacccagg tcacctccaa cgtcgagtcg
      781 gtccggtcgc tcgtctcacc gggtgagggc atgccggtgc tgagcagcac ccgcatgccg
      841 tacccgacgg tgtgacgggc ccagccccgt cgctcgccgg gctcgaggtg acgggtgcag
      901 gtgaccaggc gtccgctcgg gcgggcgccg agtccggcga gggcgccggc gagcgacgcc
      961 ctcgggtccg ggcgtgtggc cgcgagtcgg tcgaggagct cctcgaccga ctcggccgcg
     1021 ggcacgaagc cgctgtcgct cccgtcggtg agcaccagcc gcaccatctc gcggtcggtc
     1081 gccgccagga ccaccaggct ggccgcggcg gacacggccc gttcgaaggc gggatcgctc
     1141 ccgggcgtgc tgtcgtttcc gggagtgctg tcttttccgg gagtgctgtc gtttccggga
     1201 ccggtcgcag cgagggtgcg cacgtcgagc aggatcgtgc tgcggcgctg ccacggctgg
     1261 tcgtactggc gcacgaccgg ttcgccgatc cgggcggtgg tgcgccagtg gatgcgccgg
     1321 atgtcgtcgc cgacggagta ggggcgcagc gaggcgaact cctcgtcgac cgtcgacgtc
     1381 gagctcagcg cccgggtgcc gtgctcgggt tcgtcgcccg gcgcatgggg cagcgagggc
     1441 aacgtccagg tccgcggcag cacgatcagc gtccgcgcgt cgtccatcac ccggcgggag
     1501 cgcaccaggc cgaaggcgtc ctcgacgtcc acggtgagcg gacccacggg gtggaggccc
     1561 cgtcgatggg tggggaacga gtaggacgcg tcacgggtcg cgccgacggg gacgggggcg
     1621 atgccgagtc gggcccggcc gaacacgccg gcgtcgtcgt cgagtgcgag caccggggtc
     1681 cggcggcgac cgacgttgcg gatcgacagg tgcacctcgc acggctcacc gagcgcgacc
     1741 cgggccggat ggagggtgcg gcccagctcg agggtgggcc gacggctcct caccgtcacc
     1801 gcggcgacca cgagcacggc ggtggccgcg gccgccgccg cggcgagctc cggctgtccg
     1861 agcagccacg ccccggcgac caggaggggg atcgtggccg ccaccgcgac tccggctcgg
     1921 gtgggacgca cggcggtcgt ttcagcccgg gaccggggtc gtgcgcagca ggtccgcgac
     1981 cgcgtcggcc gcggtgacgc cgcggagctg ggactcggtg gcgagcagca cgcggtgggc
     2041 gaggacgggg accgccaggt ccttgatgtc gtcgggggag acgaaggtgc ggccctgcgc
     2101 cgcggcgtgc acccgggccg cgccgagcag gccgagcgcg gcccgcggcg acatccccag
     2161 ggcgaacgca ccggagcgtc ggctgcggtc ggccaactcg accaggtagg aggccaacgc
     2221 gtcggagacg aagaccccgg ccgccgcggc gcccatggcc agcacgtcct cggtgctcac
     2281 caccggcccc aacccggcga gccggtcgtc gtggtcggtc tcggtcagca gcgccgtctc
     2341 tgcgacgcgg tccgggtagc cgaccgacag gcgcatgagg aaccggtcga gctgcgactc
     2401 cggcagcggg aacgtgccct ggtgctcgag cgggttctgc gtggcgacca ccatgaacgg
     2461 tcgcggcagc gggtacgtcg tcccgtcgac ggtcacctgt tcctcggcca tcgcctcgag
     2521 cagcgcggac tgcgtcttgg gcgaggcccg gttgatctcg tcgctgagca ccaggtggtg
     2581 gaacaccgga cccgcgcgga actcgaaggc gccgctgctc tggttccaca ccgaggagcc
     2641 gagcacgtcg gagggcagga ggtccggggt gaactggacc cggccgaacc gcccgtcgac
     2701 cgaccgggcc agcgccttgg cgatcgtggt cttgccgacg ccggggacgt cctcgatcag
     2761 caggtgcccc ccggcgacca ggcacgtgac cgtcaggcgg atcacgtcgt ccttgccccg
     2821 gacggcgacg gcgatgttcg agcagatcga gtcgaacatc gccgagaacg agcggccggg
     2881 cccggccggg gtggtcgtcg ggtccatcgt ggtcatgtgg gcccgattct gccatcgcgg
     2941 cccgttcgac gggggcgtcg ccggtgccgg gagcgacccc ggggtccggt accgtcggtc
     3001 cccatggccg cggtgctcgc tctggacgtc ggggggacca agctcggcgc cgcggtggtc
     3061 gccgacgacg gacgggtcgt cgccgagggt cgacggccga ccgtcgccac cgacgccgac
     3121 gagctgttcg agctggtgac cgacctgctg gcggggtccc tcgacgccgc cgccgagcga
     3181 ggcgtgccac gcgacgacct gcgagcggtc ggggtcggtt gcggcggacc gatgaccgcc
     3241 ggcggtgagg aggtgtcgcc gctgaacatc ggggtgtggc gcggcttccc gctgcgtcgg
     3301 cggttggcgg agtggtcggg gctgccgacc ttcgtcgaca acgacgccaa ggccctggcg
     3361 ctgggcgagg ggtgggtcgg agcggcgcgt ggggtgagcg actacctggc catggtcgtc
     3421 tccaccgggg tcggcggtgg cctggtcctc gacggacggc tcctcgacgg cggagcgggc
     3481 aacgccggcc acatcggaca cgtgatcgtc gacccgctcg gtcgtcggtg cgggtgcggg
     3541 gcgaccgggt gcctcgaggc gcaggtgtcg ggcaccgcga tcgcggcgac gaccggtcgg
     3601 ccggctgccg aggccgaccc ggagatgcgg atccgggtcg ggcgactggt cggtcgggcg
     3661 gtcgcctcgg tggccaacct gctcgacctc cgtctggcgg tcgtcgccgg ctcggtggcg
     3721 ctcggcttcg gcggcgtgtt cttcgacgcg gccaacgccg agctcgaccg gtgcgcccgc
     3781 ctggagttct cccggggggc gcgggcggtg ccgtcggggt gcggagccca tggaccgctc
     3841 atcggcgcgg ccgcggtcgg ttggcgtggg attggtcacg acgtgggggt ccggtgagcc
     3901 gcggtggccg ctccgggatc ggtgtcccac cgcgggcggt gctggcggtg ctgcgtcggc
     3961 cggatctgtg gtggaccggt gtgcgccagg cgctggtgct ggcccccgac gggtggtggc
     4021 ggcgtccgcc gcacctgcca ctgcccgacc cggcctacct ccgtttccgg atggtcaccg
     4081 cccacggcgg cgacgggtc
//
LOCUS       DAOQOD010000237         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10135_length_4036_cov_11.272796, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000237 DAOQOD010000000
VERSION     DAOQOD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_4036_cov_11.272796"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLZ72_15085"
     CDS             complement(<1..593)
                     /locus_tag="PLZ72_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNA37671.1"
                     /translation="MNGIDVARVRADTPSLADRVFLNHAGSSPNPTPVLDAVIGHLRD
                     EARLGGYEAARNRADDLADVRRSIATLIGAGPDTIALATSATDAWETAFWSIPWRRGD
                     VVLTCRSEYVTNAVNLLVARDRFGVRTSVVDDDEHGQIDLTDLERHLEDPAVRLVTLC
                     HVPTHGGLVNPAAEVGRRCRDAGVAFLLDACQSVGQLP"
     gene            complement(590..1261)
                     /locus_tag="PLZ72_15090"
     CDS             complement(590..1261)
                     /locus_tag="PLZ72_15090"
                     /inference="COORDINATES: protein motif:HMM:NF039596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37672.1"
                     /translation="MADHKKRTKDDRRAERRQELLDAAVVTIRRDGAGVSMEDIAHEA
                     GITKPILYANFGDKAGLADALAKRFTRDLIARFGEVWATSDDTRERVRLAIEAWVAFI
                     ESDPHIYRFLSEGSFGAGRRLEDRRLVSDVGVVVARALGEWLREQGADSGPAEPWAYG
                     VLGMVHVTTEWWLDRRTLNRKDLVDYLTSLLWSGLSGNGLDPDRLIDQTVAPRPASDA
                     GAPGS"
     gene            complement(1254..>1730)
                     /locus_tag="PLZ72_15095"
     CDS             complement(1254..>1730)
                     /locus_tag="PLZ72_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNA37673.1"
                     /translation="LTLLAQDDSGGLQVQLPAADGDRWVDVPPVQDAFVANLGDMIER
                     LSGGRLVATPHRVAPPSTDRISMPLFLDPGWDVEISTLDGLLPPSGRIARRRWDDEDV
                     HAIEGPYSRYLLSRVSRVFPGLSDHLTPGTGSTGAVPTANRVADTGSDGETAHRDG"
     assembly_gap    1731..1830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1831..2536)
                     /locus_tag="PLZ72_15100"
     CDS             complement(<1831..2536)
                     /locus_tag="PLZ72_15100"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNA37674.1"
                     /translation="MPVLDISPLVDTAARSPLRGWDPAAARVGTELAEAAQEPGVFQV
                     VGHGVDPALRAALQDAAREFFALPDETKSTIAMTHAGRAWRGWFPIGGELTGGVPDLK
                     EGLYFGTDLDEDDPRVRAGWPLHGANLYPQHPAALARLVPEWMAAVTAVGHAVLRGLG
                     LGLGLGADAIAGWCSDPTVLFRIFHYPAIDRFVVGLDTDGVGNDGTWGVGEHTDYGLL
                     TLLAQDDSGGLQVQLPA"
     gene            2773..>4036
                     /locus_tag="PLZ72_15105"
     CDS             2773..>4036
                     /locus_tag="PLZ72_15105"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNA37675.1"
                     /translation="MKHLSRSRAGRFAAGMVAVVAACTVAGMVLLPGAQAQKVANPGT
                     FSLAPINGSMRIGTQVFDLTPRPLPQCGDGIDNDGDGLVDTADAGCSVVGAPGGSTVG
                     QDDSELAGGFQPKVDVTITGTIDGAGNISVPQSGVVFPPAYVPVLNPLDGITYVVTAK
                     VIPTAAATGILDPISGSANLQVKLRVGLTGAPAGVDLGATCSIGSDAVPLTLNLTTGS
                     SGGAFGTRYNTTTGQATMIDAAFTVPGATGCPIGLLNLNDIINQQMKIPSPSGQNLAS
                     IKGQTTPVFAKGIVARISTTPGQTSGPAPLDLSFDAGTSEVAKGPATYLWTFPDGTTQ
                     SGLTAQKTFTQPGTYVVTLTVTDGDGDKATVTKGVSVTTGSTTVPTTAAPTTAAPTTA
                     APTTAAPTTAAPTTAAPTTAAPTTAAPTT"
BASE COUNT          576 a         1375 c         1425 g          560 t
ORIGIN      
        1 gggagctgtc cgaccgactg acacgcgtcg agcaggaacg ccactcccgc gtcacggcac
       61 cgccgaccga cctcggccgc cggattcacg agtcccccgt gggtggggac gtggcagagg
      121 gtgaccaggc gcaccgccgg gtcctccagg tggcgttcca ggtctgtcag gtcgatctgt
      181 ccgtgctcgt cgtcgtcgac caccgacgtg cgcaccccga accggtcgcg ggccaccagc
      241 aggttcaccg cgttggtcac gtactcggaa cggcaggtga gcacgacgtc accccgacgc
      301 cacgggatcg accagaacgc ggtctcccac gcgtcggtgg cgctggtggc gagcgcgatc
      361 gtgtcgggcc cggctccgat gagggtggcg atggatcggc gcacgtcggc gaggtcgtcg
      421 gcccggttcc gggccgcctc gtagccgccg agtcgggctt cgtcgcggag gtggccgatc
      481 accgcgtcga gcaccggcgt cgggttgggc gacgacccgg cgtggttgag gaagacccga
      541 tccgcgagcg acggcgtgtc ggcccggacc cgggcgacgt cgatcccgtt cacgacccgg
      601 gtgctccggc gtcgctggcg ggccgaggcg cgacggtctg gtcgatcagc cggtccgggt
      661 ccaacccgtt gcccgacagg ccgctccaca gcagcgacgt caggtagtcg accaggtcct
      721 tgcggttcag ggtccgacgg tctagccacc actcggtggt cacgtgcacc atgccgagca
      781 cgccataggc ccacggttcg gccggccccg agtccgcgcc ctgctcccgg agccactcgc
      841 cgagcgcccg ggccaccacg actccgacgt cgctgaccag gcgacggtcc tcgagccggc
      901 gtccggcacc gaagctgccc tcggacagga accggtagat gtgggggtcg gactcgatga
      961 acgccaccca agcctcgatg gcgagccgga cgcgctcgcg ggtgtcgtcc gaggtggccc
     1021 agacctcgcc gaatcgggcg atgaggtcgc gggtgaagcg cttggccagg gcatcggcga
     1081 ggccggcctt gtcgccgaag ttggcgtaga ggatcggctt ggtgattccc gcctcgtggg
     1141 cgatgtcctc catcgagacg ccggcgccgt cgcggcggat ggtcacgacc gcggcgtcga
     1201 gcagctcctg tcggcgttcg gctcgacggt cgtccttggt gcgcttcttg tgatcagcca
     1261 tcgcggtgcg cagtctcgcc gtcggacccg gtgtcggcca cccggttggc ggtcgggacc
     1321 gctccggtgg atccggtgcc gggtgtgagg tggtccgaca gtccggggaa cacccgcgac
     1381 acgcggctga gcaggtagcg ggagtacggg ccctcgatgg cgtggacgtc ctcgtcgtcc
     1441 cagcgacgcc gagcgatccg acccgacggg ggcaggagac cgtcgagggt ggagatctcg
     1501 acgtcccagc cggggtccag gaacagcggc atcgagatgc ggtccgtgct cggcggagcc
     1561 acccggtgcg gtgtggcgac cagccggccc ccgctcaggc gttcgatcat gtcgccgagg
     1621 ttggcgacga acgcgtcctg aaccgggggg acgtcgaccc accggtcgcc gtcggccgcg
     1681 ggaagctgga cctggagtcc acccgagtcg tcctgggcga ggagggtgag nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcgggaag ctggacctgg agtccacccg
     1861 agtcgtcctg ggcgaggagg gtgaggagcc cgtagtcggt gtgctcaccc acgccccatg
     1921 tcccgtcgtt gccgactccg tcggtgtcga ggccgaccac gaagcgatcg atcgccgggt
     1981 agtggaagat cctgaacagc acggtcggat cgctgcacca cccggcgatc gcgtcggcac
     2041 ccagcccgag tcccagcccc aggccgcgta ggaccgcgtg gcccaccgcg gtgacggcgg
     2101 ccatccactc cgggaccagg cgggcgagcg ctgcggggtg ctgcggatag aggttggcgc
     2161 cgtgcagcgg ccagccggcc cgcacccggg ggtcgtcctc gtcgagatcg gtgccgaagt
     2221 acaggccttc cttgaggtcg gggacgcctc cggtgagctc gccgccgatg gggaaccacc
     2281 cccgccacgc ccgaccggcg tgggtcatcg cgatggtcga cttggtctca tcgggcaggg
     2341 cgaagaactc ccgggcggcg tcctggagcg cggcgcgcag cgcggggtcg acgccgtggc
     2401 ccacgacctg gaacacgccg ggctcctgag cggcctcggc gagctcggtc ccgacgcggg
     2461 cggccgccgg gtcccatccg cgcaacgggg accgggcagc ggtgtcgacg agcggggaga
     2521 tgtcgagcac cggcacctgc ccgatcgact cggtccctgg cccggtgggc ggtcccgtcg
     2581 gcgcatcccg gaacggcgag tcgaccacgg cgcaggttgg cacagggccc cgacgtgggg
     2641 cgaacgagcc gggcggacgg cgcgctccac cgacctgaac caccttcgga acggcttgac
     2701 ggtgtggccc gcggccccgc agacttgtca gattgctccg cctgtggctg tacgcggagt
     2761 ggggagggcg agatgaagca tctgagtcgg tcgcgtgcag gtcggttcgc cgccgggatg
     2821 gtggcggtgg tcgcggcgtg caccgtcgcc ggaatggtgc tcctgcccgg tgcgcaggcc
     2881 cagaaggtgg ccaacccggg gaccttctcg ctcgctccga tcaacgggtc catgcggatc
     2941 ggcacccagg tgttcgacct caccccgcgc ccgctgcccc agtgcggaga cggcatcgac
     3001 aacgacggtg acggcttggt cgacacagcg gacgccgggt gcagcgtggt cggcgctccc
     3061 ggcggctcca ccgtcggcca ggacgacagc gagctcgcgg gcggcttcca gcccaaggtg
     3121 gacgtgacga tcaccggcac catcgacgga gcggggaaca tctccgtgcc gcagtccggg
     3181 gtcgtgttcc cgccggccta cgtcccggtc ctcaaccccc tcgacgggat cacctacgtg
     3241 gtgaccgcca aggtcatccc gaccgcggcg gcgacaggaa tcctcgaccc gatcagcggc
     3301 tcggccaacc tccaggtgaa gctgcgggtc gggctcaccg gtgcgccggc aggggtggat
     3361 ctgggcgcga cgtgctccat cggttccgac gcggtgccgc tgacgctcaa cctgacgacg
     3421 gggagctccg gtggggcgtt cggcacccgc tacaacacga ccaccggtca ggcgacgatg
     3481 atcgatgccg cgttcacggt tcccggcgcg accgggtgcc cgatcggatt gctcaacctg
     3541 aacgacatca tcaaccagca gatgaagatc ccgtcgccgt cgggtcagaa cctggcgtcg
     3601 atcaagggtc agacgactcc ggtgttcgcc aagggcatcg tcgcccgcat ctcgaccacc
     3661 ccgggtcaga cgtcgggtcc ggctccgctc gacctgtcgt tcgacgccgg cacctccgag
     3721 gtcgccaagg gtccggccac ctacctgtgg accttccccg acgggaccac gcagtcgggg
     3781 ctgaccgctc agaagacgtt cacccaaccc ggaacgtacg tggtcacgct gaccgtcacc
     3841 gacggtgacg gggacaaggc gaccgtcacc aagggcgtgt cggtgaccac cgggtcgacg
     3901 accgtcccga cgactgctgc gccgacgact gctgcgccga cgactgctgc tccgacgact
     3961 gctgctccga cgactgctgc tccgacgact gctgcgccga cgactgctgc gccgacgact
     4021 gctgctccga cgactg
//
LOCUS       DAOQOD010000238         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10136_length_4036_cov_9.010801, whole genome shotgun sequence.
ACCESSION   DAOQOD010000238 DAOQOD010000000
VERSION     DAOQOD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_4036_cov_9.010801"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLZ72_15110"
     CDS             complement(<1..306)
                     /locus_tag="PLZ72_15110"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNA37676.1"
                     /translation="MTSVQFLGGGRMADALLGGLLAAGHSPSDIAVVEISPARRDELI
                     SAHPGVAVLSEPGPADALVVATKPGDAVDALATAAAAAPGLRRVLSIAAGVGTAALQA
                     "
     gene            complement(527..1000)
                     /locus_tag="PLZ72_15115"
     CDS             complement(527..1000)
                     /locus_tag="PLZ72_15115"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNA37677.1"
                     /translation="MIGAERPLTPDELAELESRIDGWLAEQLEQNPVVEAVERDDVSG
                     ERRWFVRVRGEQKAVFTIWLVLRQRNLHVETYLMPAPEENAAALYEHLLRRNLKLPDL
                     TFAIGDEDAVYVMGEVPAVWVDGEVVDRLLGSCYSVVEQCFRPAMRIGFASRFPA"
     gene            complement(997..2205)
                     /locus_tag="PLZ72_15120"
     CDS             complement(997..2205)
                     /locus_tag="PLZ72_15120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA37678.1"
                     /translation="MGSVAVVSLHTSPLAQPGTGDAGGMNVYVRELSASLAQAGVDVR
                     VYVRRWADHLPDRVRVEPGFEVVHVSAGPAELAKEDLPSVIGEFGDGVAADLAAAPVQ
                     ALHANYWLSAVAAHDLKHRLDLPLVATFHTLARVKATAGDHEPPAREAAEATVIGCCD
                     AICASNPVEADQLVQLYGARPDRIELVPSGVDHAFFSPGDRAGARAALGLDDRPTMLF
                     VGRIQPLKGLTVAVESLARLADKRTRLVVVGGPSGADGPAELRRVQERIRALGVAERV
                     QFVDPQPHHMLSSWYRAADVVVVPSRSESFGLVALEAAACGVPVVASAVGGLRTLVVP
                     GVTGMLVESRDPESFAAAIAQVLTDPVRAAAMGAAAAEESRRYSWSTTAARLRRLYAD
                     LSVRTPVACA"
     gene            complement(2299..2754)
                     /locus_tag="PLZ72_15125"
     CDS             complement(2299..2754)
                     /locus_tag="PLZ72_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNA37679.1"
                     /translation="MKEDLDRRWHDLGEFIREQRNVGQMSLRKLSEAAGVSNPYLSQI
                     ERGLRRPSAEILQQIARALEISSETLYVRAGILDEPTGQVDPIAEIRRDPSITEEQKK
                     TLVRIYQSFRRENELDDGAGSGPDGGSDGGSDEPDPDGSSDPSDVTVAS"
     gene            complement(2866..3387)
                     /locus_tag="PLZ72_15130"
     CDS             complement(2866..3387)
                     /locus_tag="PLZ72_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37680.1"
                     /translation="MDVSVEKLITFEEPADLAACSLSELRALRDDYQVVENGLSYARR
                     IVQGRLDTVAVEFERRIESDADTDLVHRLPNALAAHTRGPGLPRPGRELDPPSWADDI
                     VAAADRMLGATALADLSSVPEADLAVAVSSIAELEREVSGSRREVQRRMDRVQDEIVA
                     RYQAGASVDDLLI"
     gene            complement(3522..>4036)
                     /locus_tag="PLZ72_15135"
     CDS             complement(3522..>4036)
                     /locus_tag="PLZ72_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37681.1"
                     /translation="TGLAGGVLLLVVLALGAIVAAGAAGVADLTVPRASALAVAVLLP
                     AVPATAVVLVVGAHLWAGLFLVLAVSLYDAGCFVGGAESSSLLEGPVTGIVGVLAVTF
                     TMSVFQAPPFDTASAALVGGLCALACPVGQWVASAFLPTPDAPCRSLRRLDTYIVSAP
                     VFLAAAWALA"
BASE COUNT          607 a         1425 c         1514 g          490 t
ORIGIN      
        1 ggcctgcagc gcggcggtcc ccacccccgc ggcgatcgac agcacccgac gcaggccggg
       61 agcagccgcg gcggccgtgg ccagggcgtc gaccgcgtcg ccgggcttgg tggcgacgac
      121 gagggcgtcg gccggcccgg gttcggacag gacggccacg ccggggtggg cggagatcag
      181 ctcatcacga cgggccgggg agatctcgac cacggcgatg tcggacggtg agtgaccggc
      241 tgccagcagc ccaccgagca gagcgtcggc catccggccc ccaccgagga actgcaccga
      301 ggtcatgtcg tggagcgtac cggcggccgg aggacggacc gcttccccaa cgacccgtcc
      361 cccggctgcc ctcgacggta agggttgcgg tcggcgttgt cagcggggac cgctccccgg
      421 tgcgcgctcc ccggtcccca cccccacccc cgcgttctga cgacaagtca cgacactggc
      481 ggtactccag tgtcgtcaga aagttggttc gggggtggcg gcgggttcag gccgggaagc
      541 ggctggcgaa gccgatgcgc atggccggcc ggaagcactg ctcgaccacc gagtagcacg
      601 acccgaggag ccggtcgacg acctcgccgt cgacccacac cgccggcacc tcgcccatca
      661 cgtacaccgc gtcctcgtcg ccgatggcga aggtgaggtc ggggagcttg aggttccgtc
      721 gcagcaggtg ctcgtagagc gccgccgcgt tctcctcggg ggcgggcatc aggtacgtct
      781 cgacgtgcag gttgcgctgg cgcagcacca gccagatggt gaacaccgcc ttctgctcgc
      841 cgcggacccg cacgaaccag cggcgctcgc cggagacgtc gtcacgttcg accgcctcga
      901 ccaccgggtt ctgctcgagc tgctcggcca accagccgtc gatgcgggat tcgagctcgg
      961 ccagctcgtc gggggtgagg ggccgttccg ccccgatcac gcgcaggcca ccggggtccg
     1021 caccgacagg tccgcgtaga ggcgccgcag ccgcgccgcg gtggtcgacc acgagtagcg
     1081 ccgcgactcc tccgccgcgg cggcgcccat ggccgcggcg cgcaccgggt cggtgaggac
     1141 ctgtgcgatg gccgccgcga acgactcggg gtcacggctc tcgaccagca tgccggtcac
     1201 cccgggcacc accagggtgc gcagaccgcc gacggcggag gcgaccaccg ggacgccgca
     1261 cgcggcggct tcgagggcga ccaacccgaa cgactccgac cggctgggca ccaccacgac
     1321 gtcggcggcc cggtaccagc tcgagagcat gtggtgcggt tgcgggtcga cgaactggac
     1381 gcgttcggcc acgcccaggg cccggatccg ctcctgcacc cgccgcaact cggccggtcc
     1441 gtcggctccg ctcggtccgc cgacgacgac gagccgggtg cgcttgtcgg cgaggcgggc
     1501 gagcgactcg accgcgacgg tgaggccctt gaggggctgg atgcgaccga cgaacagcat
     1561 ggtcggccgg tcgtcgaggc cgagggcggc gcgggcaccg gcgcggtcgc ccggggagaa
     1621 gaaggcgtgg tcgacgccgg aggggaccag ctcgatgcgg tcgggccggg cgccgtagag
     1681 ctgcaccagc tggtcggcct cgaccgggtt ggatgcgcag atggcgtcgc agcagccgat
     1741 cacggtggcc tcggcggctt cgcgggcggg cggttcgtgg tcgccggcgg tggccttgac
     1801 ccgggcgagg gtgtggaagg tggcgaccag cggcaggtcg agccggtgct tgaggtcgtg
     1861 ggccgcaacc gcggacagcc agtagttggc gtgcagcgcc tggaccgggg ccgcggccag
     1921 gtcggcagcc acgccgtcgc cgaactctcc gatgaccgac gggaggtcct ccttggccaa
     1981 ctcggccggg ccggcggaga cgtgcaccac ctcgaagccc ggttcgaccc gcacgcggtc
     2041 gggaaggtgg tcggcccagc ggcgcacgta gacccgcacg tcgacgccgg cctgggccag
     2101 cgaggccgac agctcacgca cgtagacgtt catgccgccg gcgtcgccgg tgccgggctg
     2161 cgcgagcggc gaggtgtgga gggagacgac ggcgacggaa cccacggtca gatcaatctc
     2221 gcagccggcg gaagcattcc cgcacgtcgg gaccgcgagc ggatccgatc gtagggcgcg
     2281 acccggacga gcaccgcctc aggaggcgac ggtcacgtca gaggggtccg aggagccgtc
     2341 gggatccggt tcgtcggagc cgccatcaga gccgccgtcg ggaccgctcc cggcgccgtc
     2401 gtcgagctcg ttctcccgcc ggaacgactg gtagatgcgc accagggtct tcttctgctc
     2461 ctcggtgatc gacgggtccc ggcggatctc ggcgatcggg tcgacctggc cggtcggctc
     2521 gtcgaggatg ccggcgcgca cgtagagcgt ctcgctcgag atctcgaggg cgcgggcgat
     2581 ctgctggagg atctccgccg acgggcgacg caggccacgc tcgatctggg agaggtacgg
     2641 gttggagacc cccgcggcct cggacagctt gcgcagcgac atctgcccga cgttgcgctg
     2701 ctcgcggatg aactcgccca ggtcgtgcca gcgacggtcg aggtcctcct tcatgcccgt
     2761 cgagggtatc gcgacatccg cgaaccaccg attcgccgca gcaagcgcag gctgccgggt
     2821 cgaggtcgtc cgccgccgcg gtcggccggt cgcggcccga gcgcgtcaga tcaggaggtc
     2881 gtcgaccgac gcgccggcct ggtagcgggc gacgatctcg tcctgcaccc ggtccatgcg
     2941 gcgctggacc tcccgtcggg agcccgacac ctctcgttcg agctcggcga tcgagctgac
     3001 ggcgacggcc aggtcggcct cggggaccga cgacaggtcg gccagggcgg tggcgcccag
     3061 catgcgatcg gcggcggcga cgatgtcgtc ggcccacgac ggcgggtcga gctcgcgtcc
     3121 gggtcggggc agcccgggcc cgcgggtgtg ggcggccagc gcgttgggga ggcggtggac
     3181 caggtcggtg tcggcgtcgg actcgatgcg gcgctcgaac tcgacggcga cggtgtcgag
     3241 gcggccctgg acgatgcggc gggcgtagga caggccgttc tccacgacct ggtagtcgtc
     3301 gcgcagggcg cgcagctcgg acagggagca ggcggccaga tcggcgggtt cctcgaaggt
     3361 gatcagcttc tcgaccgaga cgtccacgcc ctgcaggcta gcgacccgaa cccgctgcgc
     3421 gttcgaccga ccgcgatccg ttgtgttcgt cgtccccgat ctcgattctc atgacttctg
     3481 gtgccaccag tggcaccaga agtcatgaaa atcctggact atcaggcgag ggcccaggcg
     3541 gcggccagga agaccggcgc cgagacgatg taggtgtcga ggcggcgcag cgaccggcac
     3601 ggagcgtcgg gcgtcggcag gaacgccgag gcgacccact ggccgaccgg acacgccagc
     3661 gcgcacagcc cgccgaccag cgccgcggag gcggtgtcga acggcggagc ctggaacacc
     3721 gacatggtga aggtcaccgc cagcacgccg acgatcccgg tcaccgggcc ctcgagcagg
     3781 ctggacgact cggcgccgcc gacgaagcag ccggcgtcgt agagcgacac cgccagcacc
     3841 aggaacagac cggcccacag gtgagcgccg accaccagca cgacggcggt ggcgggcacg
     3901 gccggcagca gcacggccac cgccaacgcc gatgcccgcg gcaccgtcag gtcggccacc
     3961 cccgcagccc ccgcggcaac gatcgccccc agcgcgagca ccaccaggag cagcacaccg
     4021 ccggccagac cggtgc
//
LOCUS       DAOQOD010000239         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10338_length_3991_cov_10.792937, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000239 DAOQOD010000000
VERSION     DAOQOD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10338_length_3991_cov_10.792937"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLZ72_15140"
     CDS             complement(<1..388)
                     /locus_tag="PLZ72_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37682.1"
                     /translation="MSSPTAATAALLAPRRHLSDRQARTVAGLLDATVDELREVGYDG
                     LTVRNVARRAGVAPATAYNYFASREHLVTEVFWRRLEALPETRIDRRRTAASRAAATL
                     AEMALLVADEPELAAACTAAMLSNDPD"
     gene            482..709
                     /locus_tag="PLZ72_15145"
     CDS             482..709
                     /locus_tag="PLZ72_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37683.1"
                     /translation="MSTTTVRLDDDDEAILDELAPRYGGRSSAIRHALRELAVTHHRQ
                     DALRSFLTAWGASDGPPDEATVAAMADRYGL"
     gene            706..1080
                     /locus_tag="PLZ72_15150"
     CDS             706..1080
                     /locus_tag="PLZ72_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37684.1"
                     /translation="MILDAGFLISVDRSERSAHAFLEAAARTGTDLHTTHPVVAQVWR
                     DGSRQARLAAFLRIVHVHPLDDGPSVGLLLARSRTADVVDAHLVTCAVRVGHDILTGD
                     PDDIAPLAASFGSAAPTVHTWP"
     gene            complement(1151..2341)
                     /locus_tag="PLZ72_15155"
     CDS             complement(1151..2341)
                     /locus_tag="PLZ72_15155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNA37685.1"
                     /translation="MSTSERIEVELVAYPLVYAEQAAFKDTYGGPEGVVVLESYLYRP
                     VGVASDTVLLFMHPIGAGAYLPMVRALAKAGHHVIYCNSRYRSDSALIMEKVVLDMRA
                     AVADAKERLGYSRVVLAGWSGGGSLSLLYQEQAEAPSITSTPWGDPPDLTAAELIPAD
                     AVMLLAAHPSRHGVLTDSLDPAIVDESDPDRRDPRWDLYGPDAPAPPYDREWLAGYRA
                     AQEARNRRITAWVRGELERLRDSGRPDEERGFVVHGTMADPAVLDGTIDPNDRQVGVS
                     YLGDPRLVNNGPVGLARFCTLRSWLSQWSLDDAPADGLRCAAAITVPVLVVSNGADDV
                     CYPEMGRSLFAAVSHDDKELHVVEGATHYYLGTGPDGTDQRPKLAEAVGICTDWLRAH
                     DLAP"
     gene            complement(2350..3042)
                     /locus_tag="PLZ72_15160"
     CDS             complement(2350..3042)
                     /locus_tag="PLZ72_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNA37686.1"
                     /translation="MTAPTGVHHLAISTKDMKSQFEFFTQVMGAPMVALFEMHGVEGA
                     WHGFVRLSDTCLLSFVQIAGVEDVEPIVGVTHSGTGAGISAPGTMQHLAFDVGSEEAL
                     LAMRDRLRSNGIVTFGPIEHGMCRSIYFAGLEGLTLEAAWSAEPIEPRAWVDPVACEA
                     IGMTAADAERFMSPPSYDPPAEPVPQPPIDPSKPQMGYPRKELEFMVALSDAEVAAAA
                     STPDPPVRLEQA"
     gene            complement(3039..3710)
                     /locus_tag="PLZ72_15165"
     CDS             complement(3039..3710)
                     /locus_tag="PLZ72_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37687.1"
                     /translation="MSGRAAAPGVGAAASGPEPGTDDSRRAWLVAGQDLLRRGGFPAV
                     KLAALCELTGRTTGSFYHHFPGMSEYLGELASFFGTEQPRTVLDRLAERPPAERLAEL
                     ERLSVQLHMGALHRAMRDWATVNEPAAEAVRDADRVLLVFLRDALVELGITRADAELR
                     AEVVYALAVARIDPPWPRRSTTIDAVLGAWPATRPSAATTRPNGSRRSRPSSTTPTTK
                     QEEPS"
     gene            complement(3710..>3991)
                     /locus_tag="PLZ72_15170"
     CDS             complement(3710..>3991)
                     /locus_tag="PLZ72_15170"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37688.1"
                     /translation="VAVLGGIATSADPMADRMAVRLAAADAVRRSVAVISMVCAGAGA
                     SVYKADAPLQRLQRDVEVLKGHVIFDWDRTTELAGRVALGLPLRPADRL"
BASE COUNT          558 a         1400 c         1490 g          543 t
ORIGIN      
        1 cgtcggggtc gttggagagc atggccgcgg tgcacgccgc ggccagctcg ggttcgtccg
       61 cgaccagcaa tgccatctcg gccagggtgg ccgcggcgcg cgacgcggcg gtgcggcgac
      121 ggtcgatgcg ggtctcgggc agcgcctcca gccgtcgcca gaacacctcg gtcaccaggt
      181 gctcgcggga ggcgaagtag ttgtaggcag tggccggggc gaccccggcg cggcgggcga
      241 cgttgcggac ggtcagaccg tcgtagccga cttctcgcag ctcgtcgacg gtggcgtcga
      301 gcagtccggc gacggtgcgg gcctggcggt ccgacaggtg ccggcgcggg gcgaggagag
      361 cggcggtggc ggccgtcgga ctggacatct gtccagatgg tagtccacat cggcggggcc
      421 tgtggacctg agccgtcagc ccgcgaatcg gaccgcagca cacccgtgct acattggcct
      481 cgtgagcacc accaccgtgc gcctggatga cgacgacgag gccatcctcg acgagctggc
      541 tccgaggtac ggcggacgct ccagcgcgat ccgccacgcg ctccgcgaac tggccgtcac
      601 ccatcaccgc caggatgcgc tccgatcgtt cctcaccgcc tggggcgcca gcgacggacc
      661 ccccgacgag gcgacggtcg cggcgatggc cgaccgatac ggcctgtgat cctcgatgca
      721 gggttcctga tcagcgtcga ccggagcgag cgatccgcac acgcgttcct cgaggccgcc
      781 gcccgaaccg ggacggacct gcacacgacc catcctgtcg tcgcccaggt ctggcgggac
      841 gggagtcgcc aggctcgact ggccgccttc ctgaggatcg tgcacgtcca cccgctcgac
      901 gacgggccat cggtgggact gctcctggcc cgatcccgga ccgccgacgt cgtcgacgcc
      961 cacctggtca cgtgcgcggt ccgcgtcggc cacgacatcc tgaccggcga tccggacgac
     1021 atcgcgccgc tcgcggcatc gttcgggtcc gccgcgccga ccgtgcacac gtggccgtga
     1081 atcgtcgacc cactccgccc gggcgacgtc gacccgagtc gccactgtgt caggacggga
     1141 cgggcggtgg tcagggggcc aggtcgtggg cgcggagcca gtcggtgcag atgccgacgg
     1201 cctcggccag cttggggcgc tggtcggtgc cgtcgggtcc ggtccccagg tagtagtgcg
     1261 tggcgccctc gacgacgtgc aactccttgt cgtcgtggct gaccgcggcg aacagcgacc
     1321 ggcccatctc cgggtagcag acgtcgtccg cgccgttgga gaccacgagc accggcaccg
     1381 tgatggccgc cgcgcaccgc agaccgtcgg ccggggcgtc gtccaggctc cactggctga
     1441 gccagctgcg cagcgtgcag aaccgggcca ggccgaccgg gccgttgttc accagccgcg
     1501 ggtcgcccag gtatgacacg ccgacctgac ggtcgttggg gtcgatggtg ccgtcgagca
     1561 cggccggatc ggccatcgtc ccgtgcacga cgaagccacg ctcctcgtcg gggcgtccgg
     1621 agtcgcgcag ccgctccagc tcgccgcgca cccaggcggt gatccgccgg ttgcgggcct
     1681 cctgggcggc ccggtagccg gccagccact cgcggtcgta gggaggtgcg ggcgcgtcgg
     1741 gcccgtagag gtcccagcgg gggtcgcgtc gatcggggtc ggactcgtcg acgatcgccg
     1801 ggtcgagcga gtcggtgagc acaccatggc ggctcgggtg cgcggccagc agcatgaccg
     1861 catccgcggg gatcagctcc gcggcggtga ggtccggcgg gtcgccccat ggcgtcgagg
     1921 tgatcgacgg cgcctcggcc tgctcctggt acagcagcga caacgacccg ccgccggacc
     1981 accccgccag cacgaccctg gagtagccga ggcgctcctt ggcgtccgcg accgcggcgc
     2041 gcatgtccaa cacgaccttc tccatgatga gcgcggagtc ggagcggtag cggctgttgc
     2101 agtagatgac gtggtggccg gccttggcca gcgcgcgcac catcggcagg tacgcacccg
     2161 ccccgatcgg gtgcatgaac agcaggacgg tgtcggaggc gacaccgacc ggccggtaca
     2221 ggtacgactc cagcacgacg acgccctcgg ggccgccgta ggtgtccttg aacgccgcct
     2281 gctcggcgta cacgagcggg tacgcgacca gctcgacctc gatccgttcc gacgtcgaca
     2341 cgatggacct caggcctgct cgagccggac cggcgggtcg ggggtgctgg ccgcggcggc
     2401 gacttcggcg tcggagaggg cgaccatgaa ctcgagctcc ttgcgggggt agcccatctg
     2461 gggcttggac gggtcgatcg ggggttgggg caccggctcg gcgggcgggt cgtacgacgg
     2521 cggcgacatg aatcgctcgg cgtccgcggc ggtcatgccg atcgcctcac acgcgaccgg
     2581 gtcgacccac gcccgcggct cgatcggctc ggccgaccat gcggcctcga gcgtcaggcc
     2641 ctcgagtccg gcgaagtaga tcgagcggca catgccgtgc tcgatcgggc cgaaggtgac
     2701 gatcccgttg gagcgcagcc ggtcccgcat ggcgagcagc gcctcctcgg agccgacgtc
     2761 gaaggcgagg tgctgcatgg tgcccggggc gctgatcccg gcgccggtgc cggagtgggt
     2821 gacgccgacg atcggctcca cgtcttcgac cccggcgatc tgcacgaacg acagcaggca
     2881 ggtgtcgctg aggcggacga agccgtgcca ggcgccctcg acgccgtgca tctcgaacag
     2941 ggcgaccatc ggggcgccca tcacctgggt gaagaactcg aactgggact tcatgtcctt
     3001 ggtcgagatg gcgaggtggt gcacgccggt gggtgcggtc atgacggttc ctcctgcttg
     3061 gtggtcgggg tcgtgctcga aggtcgggaa cggcgcgatc cgttcggacg ggtcgtcgcg
     3121 gcggacgggc gcgtggccgg ccaggcgccg agcaccgcgt cgatggtggt gctccgacgg
     3181 ggccagggtg ggtcgatgcg ggcgacggcg agggcgtaga cgacctcggc gcgcagttcg
     3241 gcgtcggcgc gggtgatgcc gagctcgacg agcgcgtccc gcaggaacac gagcagcacg
     3301 cggtccgcgt cgcggaccgc ctcggccgcc ggttcgttca ccgtcgccca gtcgcgcatg
     3361 gcgcggtgca gcgcccccat gtgcagctgc acgctgagcc gctccagctc ggccaaccgc
     3421 tcggcgggcg gccgctcggc gagccggtcg agcaccgttc ggggctgctc ggtcccgaag
     3481 aacgaggcga gctcgcccag gtactccgac atgccgggga agtggtggta gaagctgccg
     3541 gtcgtgcggc cggtcagctc gcacagcgcg gcgagcttga ccgccgggaa cccgccccga
     3601 cgcagcaggt cctgaccggc gaccagccag gcgcggcggc tgtcgtcggt tccgggctcc
     3661 ggtcccgacg ccgcagcacc gacgcccggg gcagcggcgc gacccgacat cagagcctgt
     3721 ccgccggtcg cagcggcagg cccagggcca ctcgtccggc cagctcggtg gtgcggtccc
     3781 agtcgaagat gacgtggccc ttgagcacct cgacgtcgcg ctgcagccgc tgaagaggtg
     3841 cgtcggcctt gtagacgctg gcgccggcac ccgcgcacac catcgagatc accgcgaccg
     3901 agcgtcgcac cgcgtcggcg gccgccagcc gaaccgccat gcggtccgcc atcgggtcgg
     3961 ccgaggtcgc gattcccccg aggacggcga c
//
LOCUS       DAOQOD010000240         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10472_length_3962_cov_11.414384, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000240 DAOQOD010000000
VERSION     DAOQOD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_3962_cov_11.414384"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLZ72_15175"
     CDS             <1..539
                     /locus_tag="PLZ72_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37689.1"
                     /translation="ITARAPNRDLHVDGDRHAYVRNGALGTITAVHHDRRDPADDALT
                     VDFDGIGPIDIPRSFFDHHRTPAGRPEVGIDHAYALTSYAVQGSTRDVSTSRVDATAT
                     RSETYVDITRGRHANHLYLTAAVDPLDGEALPRVPPAPADLAVAERLHRSTGELTAWE
                     LAHPADQPQPVPERAVGI"
     gene            613..822
                     /locus_tag="PLZ72_15180"
     CDS             613..822
                     /locus_tag="PLZ72_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37690.1"
                     /translation="MSTTETDAPAEVLTIDEAAAILRISRNAAYAAAREWRATGGKTG
                     IPCIEIGRTLRVPRAELERLLGRSA"
     gene            893..1441
                     /locus_tag="PLZ72_15185"
     CDS             893..1441
                     /locus_tag="PLZ72_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37691.1"
                     /translation="MAQETYRRRAWELAMGQYGYVTAADARTLGIPMVELGKLAARGQ
                     IRHVAYGLYRFDDLPPTRYDQFFEAVARVGGDAHLTGDAVLALHNLALVNPRHVRVGT
                     TRRVRAKLPEWIKVVNETVDPDDLTHYELIPSTTVAHAIRACRGTVMSDRLLTAVDEA
                     RREGLVTATEERQLRQELRDSP"
     gene            1438..2307
                     /locus_tag="PLZ72_15190"
     CDS             1438..2307
                     /locus_tag="PLZ72_15190"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNA37692.1"
                     /translation="MTDPAYPTPPVHRAALEQRLRNICGSLGVNELRTRTSLAHTVVA
                     QMLPAGVVKGGAAIKLRVGDRASRLTTDLDAARAATHTIESYTAELSDQLAAGWNGFT
                     GRLVDREPADPPDVPAEYVMQPFDVKLSYAGQAYVTVPLELGHDEVGSTNQPRLALDA
                     GIAEVIERLGFPTPAPVPVLAVEHQIAQKLHACTTPDRHGGNERAHDLVDLQILVAAE
                     PPDLRELATVGRRLFAARRVAPWPPTVQAWPGWDGRYVDAAEGLDVRPLTEAVSWLND
                     LIDAAERAAGDRS"
     gene            complement(2304..2951)
                     /locus_tag="PLZ72_15195"
     CDS             complement(2304..2951)
                     /locus_tag="PLZ72_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37693.1"
                     /translation="MDATDENSISVLGRDLLLVDVGGGAETHLAEVADGQGRSARHQG
                     NEDAPPQGRWNYSTLCGRTWNRMAAGADELLPLWRDPAFAPTCRRCLRLLDSWFPTAE
                     VPSGVWLLASVVAEEVTRFSSTYVTGVPVQHVEAKRAAIRKALRSAGFRSSTRVVEGV
                     VHVWSDDAYDALDPAEIGASVTSAIDRITSGADTASPRDPDSTPGPVDWHTWVVD"
     gene            complement(3061..3423)
                     /locus_tag="PLZ72_15200"
     CDS             complement(3061..3423)
                     /locus_tag="PLZ72_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37694.1"
                     /translation="MSGPVDAAEQAAESVRAVNHLTLRAPSPGTPGWEDVGDLYRVLG
                     ELRVLAERLPQACSQLARHLERPATGRAYEVDAMADQPAEVVVASAVLALDDVERCAS
                     RTGEHLNEALSAVAHLHP"
     gene            complement(3420..>3962)
                     /locus_tag="PLZ72_15205"
     CDS             complement(3420..>3962)
                     /locus_tag="PLZ72_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNA37695.1"
                     /translation="AYEVSISDVKTGTSRRTIDIDSDVVQVLRDWFKTRTEERDGTEP
                     AADDLMFTKDDGSWLHPDTFSQLFDRTVAKAKVPVISLHDLRHTHATLLLKAGVHVKV
                     VSERLGHANVAFTMNVYQHVLPGMQAAAADTFSLLLRNQRNKAASEAEPESEPDDEAE
                     AEVEVDGETDVDGASNEDDQ"
BASE COUNT          622 a         1526 c         1246 g          568 t
ORIGIN      
        1 gcatcaccgc ccgcgccccc aaccgcgacc tccacgtcga cggcgaccgg cacgcctacg
       61 tccgcaacgg cgccctcggc accatcaccg ccgtccacca cgaccgacgc gaccccgcag
      121 acgatgcact caccgtcgac ttcgacggca tcggccccat cgacatcccc cggtcgttct
      181 tcgaccacca ccgcaccccc gccggacgcc ccgaggtcgg catcgaccac gcctacgccc
      241 tcacctccta cgcagtccaa ggctccaccc gcgacgtctc caccagccgg gtcgacgcca
      301 ccgccacccg ctcggagacc tacgtcgaca tcacccgcgg ccgtcacgcc aaccacctct
      361 acctcaccgc cgcggtcgac cccctcgacg gcgaagcact cccacgcgtc ccacctgctc
      421 ccgccgacct cgccgtcgcg gaacggctcc accgctccac cggagaactc accgcctggg
      481 aactcgctca ccctgccgac caaccacagc ccgtaccaga gcgcgcagtc ggaatctgag
      541 attccccgcg aagtcctggc ccgccaaagg gcctacgtag gtcagagaac agcaccgacc
      601 gaggaggacc cgatgtccac caccgagacc gacgccccgg ccgaagtcct caccatcgac
      661 gaggccgccg ccatcctgcg catcagccgc aacgccgcct acgcggccgc tcgcgagtgg
      721 cgagccaccg gcgggaagac gggtatcccg tgcatcgaga tcggccgcac gctccgcgtc
      781 ccccgcgccg aactcgaacg gctgctcggc cgctctgcgt gagagacttg ggccaccgcc
      841 acttgggtaa ctggtggcgg ctgaacttta ccgaaagcgg taaagttcag ccatggccca
      901 ggagacgtac cgacgtcgcg cgtgggagtt ggcgatgggc cagtacggct acgtgaccgc
      961 cgcggatgcc cgcacgctcg gcatcccgat ggtcgagttg ggcaagctcg cggcgcgtgg
     1021 ccagatccgc cacgtcgcct acggcctcta ccgcttcgac gaccttcccc cgactcgcta
     1081 cgaccagttc ttcgaggccg ttgcccgcgt cggcggcgat gctcacctga ccggcgacgc
     1141 cgtgctcgcc ctccacaacc tggcgctcgt taacccccgc cacgtcagag tcggcacgac
     1201 acggcgggtg cgtgccaagt tgcccgagtg gatcaaggtc gtgaacgaga cggtcgaccc
     1261 tgacgacctc acccactacg agctgatccc ctcgaccact gtcgcccacg cgatccgtgc
     1321 ctgccggggc acggtcatgt cggaccgtct gctcaccgcc gtcgacgaag cccgccgaga
     1381 ggggctcgtc accgcgaccg aagaacgcca actccgccag gaactccgag actcgccatg
     1441 accgatcccg cctatccgac accgccggtc catcgtgccg cgctcgaaca acgcctccgc
     1501 aacatctgcg gctcgctcgg cgtcaacgag ctgcgtaccc gcacgtcgct cgcgcacacc
     1561 gtcgtcgccc agatgctccc cgccggggtc gtcaagggcg gcgccgcgat caagctccgc
     1621 gtcggcgacc gggccagccg actcaccacc gacctcgacg ccgcacgcgc cgccacccac
     1681 accatcgaga gctacaccgc ggagctctcg gaccagctcg ctgcgggatg gaacggcttc
     1741 accggacgac tcgtcgaccg cgagcccgcc gatccgccgg acgtgccagc cgagtacgtg
     1801 atgcaaccct tcgacgtgaa gctgtcctac gcaggccagg cctacgtcac ggttccgctc
     1861 gagcttggcc acgacgaagt cggctccacc aaccaacccc ggctcgcgct cgacgctggc
     1921 atcgcggagg tgatcgaacg gctcggcttc ccgacacctg cgccggtgcc cgtgctcgcg
     1981 gtggagcacc agatcgcgca gaagctccat gcctgcacca caccggaccg ccacggcggc
     2041 aacgagcgcg cgcacgacct cgtggacctc cagatcctcg tcgccgccga accacccgac
     2101 ctccgcgaac tcgcgacggt cggacgacgc ctgttcgccg cccgccgcgt cgcaccatgg
     2161 ccaccgaccg tccaggcgtg gcccggctgg gacggacgct acgtcgatgc cgcggaaggg
     2221 ctcgacgtgc gcccgcttac cgaagccgtc tcctggctca acgacctcat cgacgcggcc
     2281 gagcgagccg ccggggatcg ttcctagtcc acgacccagg tgtgccagtc gaccgggccg
     2341 ggcgtcgagt cgggatcgcg cggagacgca gtgtcagccc cgctggtgat ccggtcgatg
     2401 gctgaggtca cgctggcgcc gatctcggct gggtcgagcg cgtcgtacgc gtcgtcggac
     2461 cacacgtgca cgacgccctc gacgaccctc gtgctcgaac ggaacccagc ggagcgcagc
     2521 gccttgcgga tcgcggcgcg cttcgcctcg acgtgttgga ccgggacgcc ggtgacatag
     2581 gtggagctga accgggtgac ctcctcggcc accaccgagg cgagcagcca cacacccgac
     2641 ggcacctccg cggtcgggaa ccacgaatcg aggaggcgta ggcacctgcg acaggtcggg
     2701 gcgaacgccg ggtcgcgcca cagcggcaac aactcgtcgg caccggcagc catccggttc
     2761 caggtccggc cacacagcgt cgagtagttc cagcgaccct gaggcggggc gtcctcgttg
     2821 ccctggtgtc gcgcggaccg gccttggccg tcggcgacct cggcgaggtg ggtttcggcg
     2881 ccgccgccga cgtcgacgag cagcaggtcc cggcccagca ccgagatcga gttctcgtcg
     2941 gttgcgtcca tcggtggctc ctcgcgcggc tgaccgctcc agtttcgccg ccgcgtcggt
     3001 attcggtgtg ccactcctgc cggtggctcc ggggtctgtc gaccccggag ccagtccggc
     3061 ctatggatgg aggtgcgcga ctgccgatag ggcctcgttg aggtgttcac cggtgcggga
     3121 cgcgcagcgc tcgacgtcgt cgagggccag caccgcggaa gcgaccacga cctcggcggg
     3181 ctggtcggcc atggcgtcga cctcgtaggc acgacctgtt gcgggccgtt cgaggtgccg
     3241 cgccaactgg ctgcacgctt ggggcaggcg ctcggccagg acccgcagct cgccgagcac
     3301 ccggtagaga tcgccgacgt cctcccagcc cggcgtgccc ggagaaggtg cgcgaagggt
     3361 gaggtggttc accgctcgca ccgactccgc tgcctgctcg gcggcgtcga ccgggccgct
     3421 cactgatcgt cctcgttgct cgcgccgtcg acgtcagtct cgccgtcgac ctcgacctcg
     3481 gcctcggcct cgtcgtcggg ctcggactcg ggctcggctt cggatgccgc cttgttgcgc
     3541 tggttgcgca gcagcaacga gaacgtgtcc gccgcggcgg cctgcatgcc cggcaggacg
     3601 tgctggtaca cgttcatggt gaacgcgacg ttggcgtggc cgagccgttc gctgaccacc
     3661 ttcacgtgca cgcctgcctt cagcagaagc gtggcgtggg tgtggcgcag gtcgtgcaac
     3721 gagatcaccg gcaccttggc cttggcgacg gtgcggtcga agagctggct gaaggtgtcg
     3781 gggtgcagcc acgagccgtc gtccttggtg aacatcagat cgtccgccgc cggctcggtc
     3841 ccgtcgcgtt cctcggtgcg ggtcttgaac cagtcccgca gcacctgcac gacatcggag
     3901 tcgatgtcga tcgtgcggcg gctcgtcccc gtcttcacgt ccgagatcga cacctcgtac
     3961 gc
//
LOCUS       DAOQOD010000241         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10696_length_3915_cov_12.018912, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000241 DAOQOD010000000
VERSION     DAOQOD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_3915_cov_12.018912"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1799
                     /locus_tag="PLZ72_15210"
     CDS             81..1799
                     /locus_tag="PLZ72_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA37696.1"
                     /translation="MTRPSSAPTWAGALAGIVAAGCGIAVGELVAGVDARLRGPIAAV
                     GDRVIDTVPAPVKTWAIDTFGTNDKAVLLTGVTVLLAVFAVVVGVIALRRSLRAGLVG
                     VGLFAGIGVLASLGRGGTGWARPVPPIVAGLVAGWVLVLLTRRAAQTWPAPTTDAASP
                     DPEHVDAPQPMAAGPPTPAARPTGAEQPTTEPMTVPAAGTDRRRFLRLTAGLAGAAVA
                     AGGVGRWLQSRAVAVAERLEGVLPRAAHSLPPVPAGVQARGAAPFITPNADFYRIDTA
                     LVVPQVSHDGWTLGVTGRVRHPLTLTYAELLDRPMVEADITISCVSNEVGGDLIGTAR
                     WLGCRLDELLDEAGIDPSADQVVGRSVDGFTAGFPTALLDGRPALVAVGMNGEPLPID
                     HGYPARLIVPGVYGYVSATKWLAEIELTRFDEFDAYWIPRGWSQRAPIKTQSRIDTPH
                     DGAAVTVGERRAIAGVAWAMDRRISAVEVRISPPDRDAGAAAGTESDPAGSAEGEWQP
                     AVLADELNDTTWRQWHLPWTPARAGRYTISVRATDGTGATQPEERTDVAPDGATGWHT
                     ITVTAA"
     gene            1839..2348
                     /locus_tag="PLZ72_15215"
     CDS             1839..2348
                     /locus_tag="PLZ72_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HNA37697.1"
                     /translation="MPYPGDRFPLERRRHRGGVGDGGECTMALSERSRSVLYHGFMSV
                     VDDEAAVQEMMAFFPARDLEEPVTRDHLHAELADLRIEMAELRTEFAEFRTDMRSEMD
                     EFRTDMRSEMDAFRNEIRAEMDAFRTDIRTELGTMRTEFRSELHKVFGWNLAAFVSMA
                     AVIVAAVRL"
     gene            2540..3847
                     /locus_tag="PLZ72_15220"
     CDS             2540..3847
                     /locus_tag="PLZ72_15220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNA37698.1"
                     /translation="MSSIAVVGTGYVGLTTAASMASLGHSVVGIDIDADKVAKLTAAE
                     VPILEPGLTELVRDGLRQGHLRFSTDHAEAAHVEFVFLCLPTPPAENGRADLTYLLDA
                     ARTLSPHLKADTVVINKSTVPVGSCARVQRELGRQDVFVVSNPEFLREGHAVQDCLQP
                     DRLVIGSDDEAAAIRVFSLYRGVVTPLVVTDPATAELTKYAANAFLATKLSFVNSIAA
                     ICEEVGADIGDVVLGLGHDKRIGAEFLRPGPGWGGSCFPKDTRALDAIARDAGYDFTL
                     LRETIAVNDEQKHRVVDKVRRLLDGPLEGARIAVWGLTFKAGTDDLRESPSLAVIAEL
                     VAAGADVHAHDPTVDPGTDLSRATGIAAVGPDALTIHKDALDAADGAQALVVMTEWDD
                     YRWIDPAELVERMAAPGVVDTRNLLNRSSLARAGCRLLSTGRA"
BASE COUNT          538 a         1486 c         1336 g          555 t
ORIGIN      
        1 cccgctttgt cccgcacaga cgcggtgatc accgcgtctg tgcgtgacaa agcgggggtg
       61 gaggggtcca gactggtggg gtgacccgtc cgtcatcggc cccgacgtgg gccggagcgc
      121 tcgccggcat cgtcgccgca gggtgcggga tcgcggtcgg agagctggtc gccggtgtcg
      181 acgcccggct ccgtggcccc atcgccgcgg tcggtgaccg ggtgatcgac actgtccccg
      241 ccccggtgaa gacgtgggcg atcgacacgt tcggcaccaa cgacaaggcg gtcctcctca
      301 ccggggtgac cgttctgctg gcggtgttcg ccgtggtcgt cggcgtgatc gcgcttcgcc
      361 ggtcgctccg agcgggcctg gtcggtgtag ggctgttcgc ggggatcggc gtgctcgcct
      421 cgctcggtcg gggcggaacg ggctgggccc gtccggtccc gccgatcgtg gcgggtctcg
      481 tggccgggtg ggtgctggtc ctcctcaccc gccgcgccgc gcagacctgg cccgcaccga
      541 cgacggacgc cgcatcgccc gaccccgagc acgtcgacgc cccgcaaccc atggcggccg
      601 gaccgcccac accggctgcg cgacccaccg gcgccgagca acccaccacc gagccgatga
      661 ccgtcccggc tgccgggacc gaccgcaggc ggttcctgcg tctcaccgcc ggactggccg
      721 gtgccgcggt cgccgccggg ggagtgggcc gctggctgca gagccgggcg gtcgccgtcg
      781 ccgagcgcct cgagggcgtc ctgccccgcg ccgcccactc gctcccgccc gtccccgccg
      841 gtgtccaggc ccggggagcg gcgccgttca tcacccccaa cgccgacttc taccggatcg
      901 acaccgccct ggtcgtgccc caggtcagcc acgacggctg gaccctcggc gtcaccggcc
      961 gggtgcggca cccgctcacc ctcacctacg ccgagctgct cgaccgtccg atggtcgagg
     1021 ccgacatcac gatcagctgc gtgtccaacg aggtgggcgg cgacctgatc ggcacggccc
     1081 gctggctcgg ctgtcgcctg gacgaactgc tcgacgaggc cggcatcgac ccgtccgccg
     1141 atcaggtcgt cggccgctcc gttgacggct tcaccgccgg gttccccacc gcgctcctcg
     1201 acggccgccc ggcgctggtc gcggtcggca tgaacggcga gccgttgccg atcgaccacg
     1261 gctaccccgc gaggctgatc gttcccggcg tctacggcta cgtgtcggcg accaagtggc
     1321 tggccgagat cgagctcacc cgcttcgacg agttcgacgc ctactggatc ccccgagggt
     1381 ggtcgcagcg ggcgccgatc aagacccagt cccgcatcga cactccccac gacggcgccg
     1441 cggtcaccgt cggtgagcgc cgggcgatcg ccggggtcgc ctgggcgatg gaccgtcgca
     1501 tctccgcggt cgaggtgcgg atctcccctc cggaccgcga cgccggcgca gcggcgggca
     1561 ccgagagcga cccggccgga tctgccgagg gggagtggca gcccgccgtc ctggccgacg
     1621 agctcaacga caccacctgg cgccagtggc atctgccctg gaccccggcg cgggcgggtc
     1681 ggtacacgat ctcggtgcgg gccaccgacg gcaccggagc gacccaaccc gaggagcgca
     1741 ccgacgtcgc ccccgacggc gccaccggtt ggcacaccat caccgtcacc gcggcctgag
     1801 cccccgcgat gggtgccgct ggaccgggac gcaggtccat gccctaccct ggtgaccgct
     1861 tccccctcga gcggcgccgc caccgcggcg gcgtcggtga tggcggggag tgcacgatgg
     1921 cgttgagcga acggagccgg tcggtcctct accacgggtt catgtccgtg gtggacgacg
     1981 aggcggcggt gcaggagatg atggccttct ttccggctcg tgacctggag gagcccgtga
     2041 cccgggacca cctgcacgcg gagctggccg acctgcgcat cgagatggcg gagctgcgga
     2101 ccgagttcgc cgagttccgc accgacatgc gctccgagat ggacgagttc cgcaccgaca
     2161 tgcgctccga gatggatgcg ttccggaatg agatccgggc ggagatggat gcgttccgca
     2221 ccgacatccg caccgagctg ggcactatgc gcacggagtt ccgctccgag ctgcacaagg
     2281 tgttcggctg gaacctggcc gcgttcgtgt cgatggctgc ggtcatcgtc gccgcagtgc
     2341 gcctctgacc ctctcgggca ccggtctccc cgacgagcgc gtccacccgc gtggcggcgc
     2401 acacgcccgc atcggccaac ctcacgcatg cgccgagacg tccggacatt gggagaaccg
     2461 cccgcttgat cgccccgatc gatccgggca cccttgaacc cggccgaacg tcgttcgggc
     2521 agaaggggac acgcacgcca tgagcagcat cgccgtcgtc ggcaccgggt acgtgggtct
     2581 gaccacggcc gcgtccatgg ccagcctcgg ccacagcgtc gtcggcatcg acatcgacgc
     2641 cgacaaggtc gccaagctca cggcggccga ggtccccatc ctcgagcccg gcctcaccga
     2701 gctcgtcagg gacggcctcc gtcaggggca cctgcgcttc tcgaccgacc acgccgaggc
     2761 cgcgcacgtc gagttcgtct tcctctgcct gcccacgccg ccggcggaaa acggtcgggc
     2821 ggatctcacc tacctgctcg acgcggcgcg caccctgtcg ccgcacctca aggccgacac
     2881 ggtcgtgatc aacaagtcga cggtcccggt cggctcctgc gcccgggtgc agcgcgagct
     2941 cggccgccag gacgtgttcg tcgtgtccaa ccccgagttc ctgcgagagg gccacgccgt
     3001 gcaggactgc ctgcagccgg accgtctggt catcggcagc gacgacgaag ccgccgccat
     3061 ccgggtcttc tccctgtacc ggggcgtcgt cacgccgttg gtggtcaccg accccgccac
     3121 tgccgagctc accaagtacg cggccaacgc cttcctcgcc accaagttga gcttcgtgaa
     3181 ctcgatcgcg gcgatctgcg aggaggtcgg cgccgacatc ggcgacgtcg tgctgggcct
     3241 cggtcacgac aagcgcatcg gcgccgagtt cctgcgtccc ggcccgggtt ggggcggctc
     3301 gtgcttcccc aaggacaccc gcgctctcga cgccatcgcc cgtgatgccg gctacgactt
     3361 cacgctcctt cgggagacga tcgcggtcaa cgacgagcag aagcaccggg tcgtcgacaa
     3421 ggtccgacga ctgctcgacg gcccgctcga gggtgcacgg atcgcggtgt ggggtctcac
     3481 gttcaaggcc ggcaccgacg acctgcgcga gtcgccgtcg ctggcggtca tcgccgagct
     3541 ggtcgccgcc ggcgcggacg tgcacgcgca cgacccgacg gtcgatccgg gcacggacct
     3601 gagccgcgcc accggaatcg ccgcggtcgg ccccgacgct ctcaccatcc acaaggacgc
     3661 gctcgacgcc gctgacggcg cgcaggccct ggtggtcatg accgagtggg acgactaccg
     3721 ctggatcgac ccggccgagc tggtcgagcg gatggccgcc cccggggtgg tcgacacccg
     3781 caacctgctg aaccggtcgt cgctggcccg ggccggctgc cggctcctgt ccaccggccg
     3841 cgcctgaccc cacccactcc ctcccacccc accccctccc atcgttctgt agtgcgagat
     3901 ccaggcgaac gcctg
//
LOCUS       DAOQOD010000242         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10726_length_3909_cov_8.442916, whole genome shotgun sequence.
ACCESSION   DAOQOD010000242 DAOQOD010000000
VERSION     DAOQOD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_3909_cov_8.442916"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="PLZ72_15225"
     CDS             <1..2186
                     /locus_tag="PLZ72_15225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA37699.1"
                     /translation="ARDGHVRLRWDLPEWPRASVVVPTRHNRPLLESLVPTLRGTDYP
                     DWELVVIDNGGRSDERQDYYDHLLDGLDARVIWWDEPFNYGTVNNHAVAQTDGDVVVL
                     LNDDTQTRSADWLTELVGWATRDEIGTVGVQLVNGDGVIQHGGVVIGMSGFANHLFAG
                     LPPHTDTVLGSTDLYRDTSANTAACVALRRSVWDRIGGLDERFDLLGSDVVLGLDAMG
                     LGLRNVTTPAIDVRHLESVTRGADVPVHDMYASYWRYHRWLKVGDPYFPRALSLLGPD
                     LRLRSGHEPSALEQVGPLLGRQFGVFRQTASEAEALMLADLCRLPDARIDELAAEHAG
                     VGGSRPVKTVNWFIPDVENPFYGGIATAFRIAEHLRVNHGVENRFVVWSNPNEEWFRS
                     AIRAIFPGLADSPIVFHDGSLGERLDDLPPCDVAIATQWPTAYAVAAFRGATRRFYLV
                     QDFEPVFHPAGTLYALAEESYKLGLYGICNTVSMGRFYREDYGGVGSHFVPAVDTEVF
                     HSRGRPVRDPDDPIRLFLYARPGHWRNCWELVSLALDELKAAYGRRLTVVTAGSWARP
                     EDLGRGIEHLGLLDYRATGDLYRSCDIGISLTVSPHPSYLPLELMACGAAVVAFDLPP
                     GYWILDDGVDCLLSRRTVPSLVSNVGRLIDDDALRGRLQRGGLERIARHHSDWDSALS
                     RIYHQLCDPEAFDQRDRRLDALDDGPVTGQVLRGLAPVPGAWAPG"
     gene            2196..3557
                     /locus_tag="PLZ72_15230"
     CDS             2196..3557
                     /locus_tag="PLZ72_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA37700.1"
                     /translation="MSLRFVIIGGGPAGTQAATSAARLGADVTLIERDIVGGAAHLWD
                     CVPSKAMIATGGRLDTIEDSNGMGLRVTDASVDLEAVRERIARITDRLEHHNRTLLES
                     QGVALVRGTGRLLDDHTVVATTEAGELTFEADAIMVSTGSRPRIPDWASPDGDRVLTT
                     RDSYPPKVLPEHVIVIGSGVTGVEFVHMFSSFGCRVTLIVSRQQVLPTKDPEVAAVLE
                     EDFLQRGVRLYKGARAIGIEHTEGGVSVRCDDGRVAEGTHCVLAIGSIPNTEGIGLAE
                     AGVAMDGPWVADLDHHLRTNVAHIYAAGDISGKLPLSSVATMQGRKIAEHVMGLHKVD
                     HRHLDYEKAASAIFTDPEIADVGLAEADAFAEGRKVRVTKVPFASSAKALINDDPRGF
                     VKIISDPATGVILGGSIVGPQAAELIAVLAVAVTNGLRVSDIAESIFVHPALAEALTD
                     AAE"
     gene            complement(3574..>3909)
                     /locus_tag="PLZ72_15235"
     CDS             complement(3574..>3909)
                     /locus_tag="PLZ72_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNA37701.1"
                     /translation="VALYVPESQLAPVAGGPAAKPRPKRGGSSSAGAGAAGSGKIAVP
                     MQGTIVKINVEVGQTVAAGEAVVVLEAMKMENNVAADIDGTVTAVNVEAGQSVAAGEV
                     VVVIEAADA"
BASE COUNT          560 a         1466 c         1291 g          592 t
ORIGIN      
        1 tggcccgcga cggacacgtc cggctgcggt gggacctgcc ggagtggccg cgggcctcgg
       61 tcgtggtgcc cacccgccac aaccgaccgc ttctcgagtc gctcgtcccc acgctccgcg
      121 gcaccgacta ccccgactgg gagctggtcg tcatcgacaa cggcggtcgc agcgacgagc
      181 gccaggacta ctacgaccat ctgctcgacg gcctcgacgc ccgggtgatc tggtgggacg
      241 agccgttcaa ctacggcacc gtcaacaacc acgccgtggc gcagaccgac ggcgacgtcg
      301 tcgtgctgct caacgacgac acccagaccc gctcggcgga ctggctcacc gagttggtcg
      361 gttgggccac tcgcgacgag atcggcacgg tcggggtgca actggtgaac ggcgacggcg
      421 tgatccagca cggtggcgtg gtcatcggca tgtcagggtt cgccaaccac ctgttcgccg
      481 ggctcccgcc ccacaccgac acggtgctcg gatcgaccga cctctaccgc gacacctcgg
      541 ccaacaccgc cgcctgcgtg gccttgcgtc ggtcggtgtg ggaccggatc ggcggcctcg
      601 acgagcgctt cgacctgctc ggaagcgacg tcgtgctggg cctggacgcc atgggactcg
      661 gcctgcgcaa cgtcaccacc cccgcgatcg acgtgcgcca cctcgagtcg gtcacccggg
      721 gagccgacgt accggtccac gacatgtacg ccagctactg gcgttaccac cggtggctga
      781 aggtcggcga cccgtacttc ccccgcgctc tcagcctgct cggacccgac ctgcgcctgc
      841 gctccggcca cgagccgtcc gcactcgaac aggtcggtcc cttgctgggc cggcagttcg
      901 gcgtcttccg ccagaccgcg agcgaggccg aggcgctgat gctggcggac ctgtgccgcc
      961 tgcccgacgc tcggatcgac gagctcgccg ccgaacacgc gggcgtgggc ggttcccgtc
     1021 cggtcaagac ggtcaactgg ttcatccccg acgtcgagaa ccccttctac ggcgggatcg
     1081 ccaccgcgtt ccgcatcgcc gagcacctcc gggtcaacca cggcgtcgag aaccgcttcg
     1141 tggtctggtc caacccgaac gaggagtggt tccgttccgc catccgggcc atcttcccgg
     1201 gcctggccga ctcgccgatc gtgttccacg acggcagcct gggcgaacgc ctggacgacc
     1261 tgccgccctg cgacgtcgcc atcgccaccc agtggcccac cgcctacgcg gtggccgcat
     1321 tccgcggcgc gacccgacgg ttctacctgg ttcaggactt cgagccggtg ttccacccgg
     1381 ccggaaccct gtacgccctg gccgaggagt cctacaagct cggcctttac ggcatctgca
     1441 acaccgtgtc gatgggccgc ttctaccggg aggactacgg cggggtcggg tcgcacttcg
     1501 tccccgcggt cgacaccgag gtgttccact cccggggccg tcccgtccgg gatcccgacg
     1561 acccgatccg gctgttcctg tacgcccgtc ccggacactg gcgcaactgc tgggagctgg
     1621 tgtcgctcgc gttggacgag ctcaaggccg cctacggtcg tcgcctcacg gtcgtcaccg
     1681 ccggctcgtg ggcccggccc gaggacctcg gtcggggcat cgagcacctc ggcctcctcg
     1741 actaccgggc caccggcgac ctctaccgtt cctgcgacat cgggatctcg ctcacggtgt
     1801 cgccgcaccc gtcgtacctg ccgctcgagc tgatggcctg cggtgctgcg gtggtggcgt
     1861 tcgacctgcc gccgggctac tggatcctcg acgacggcgt ggactgcctg ctcagccgcc
     1921 ggacggtccc gagcctggtg tccaacgtcg ggcggctgat cgacgacgac gccctgcgcg
     1981 gccgactgca acgtggcgga ctcgaacgga tcgcccgcca ccactccgac tgggactcgg
     2041 cgctgtcgcg gatctaccac cagctgtgcg atcccgaggc gttcgaccaa cgcgaccgac
     2101 gactcgacgc cctcgacgac ggcccggtga ccggtcaggt cctgcgtggc ctggctccgg
     2161 tgccaggggc gtgggccccc ggatagcctg aacccatgtc cctgcgcttc gtgatcatcg
     2221 gcgggggccc ggccggcacc caggccgcga cctcggcggc ccgcctcgga gcggacgtca
     2281 ccctcatcga acgcgacatc gtcggcgggg ccgcgcacct gtgggactgc gttccgtcca
     2341 aggccatgat cgccaccggc ggacgtctcg acacgatcga ggactccaac ggcatgggcc
     2401 tgcgcgtcac cgacgcctcg gtcgacctcg aggcggtgcg cgagcgcatc gcccgcatca
     2461 ccgaccggct cgagcaccac aaccggaccc tgctcgagag ccagggcgtc gccctggtcc
     2521 gcggcaccgg tcgcctgctc gacgaccaca ccgtggtggc gaccaccgag gcgggtgagc
     2581 tcaccttcga ggccgacgcc atcatggtct cgaccggtag ccgtccccgc atccccgact
     2641 gggcctcacc cgacggcgac cgggtgctca cgacccggga ctcctacccg cccaaggtcc
     2701 tgcccgagca cgtcatcgtg atcggctccg gcgtgaccgg cgtcgagttc gtccacatgt
     2761 tctcgagctt cggctgtcgg gtcaccctga tcgtgtcgcg ccagcaggtg ctgccgacca
     2821 aggaccccga ggtggccgcc gtgctcgagg aggacttcct gcagcgcggc gtccggctct
     2881 acaagggagc ccgcgccatc gggatcgagc acaccgaagg gggagtgtcg gttcgttgcg
     2941 acgacggtcg cgtcgccgag ggcacgcact gcgtcctcgc catcggctcc atccccaaca
     3001 ccgagggcat cggactggcc gaggccgggg tggccatgga cgggccgtgg gtggcggacc
     3061 tggaccacca cctgcgcacc aacgtggcgc acatctacgc agcgggcgac atctccggca
     3121 agctgccgct cagctcggtg gcgacgatgc aggggcgcaa gatcgccgag cacgtgatgg
     3181 gtctccacaa ggtcgaccac cgccacctcg actacgagaa ggcggcgtcg gccatcttca
     3241 ccgaccccga gatcgcggac gtcgggctgg ccgaggccga cgccttcgcc gagggccgca
     3301 aggtcagggt caccaaggtc ccgttcgcat cgtcggccaa ggcgctgatc aacgacgacc
     3361 cccgagggtt cgtcaagatc atctcggacc cggccaccgg cgtgatcctc ggcggttcga
     3421 tcgtcgggcc ccaggcggcg gagctgatcg cggtgctcgc cgtggcggtc accaacggtc
     3481 tgcgggtcag cgacatcgcc gagagcatct tcgtgcaccc cgcgctggcc gaggcgctca
     3541 ccgacgccgc ggagtgaccc cgcatcttga gcgtcaggcg tcggccgcct cgatgacgac
     3601 cacgacctcg cccgcggcga cggactgtcc ggcctcgacg ttcacggcgg tgacggtgcc
     3661 gtcgatgtcg gcggcgacgt tgttctccat cttcatggcc tcgagcacga cgacggcctc
     3721 accggcggcg acggtctggc cgacctcgac gttgatcttg acgatcgtgc cctgcatcgg
     3781 aacggcgatc ttcccgctac ccgctgctcc ggcccccgcg ctggagctgc cgccccgctt
     3841 gggccggggc ttggcggccg ggccgccggc caccggtgcc agttgcgact cgggcacgta
     3901 gagcgccac
//
LOCUS       DAOQOD010000243         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10751_length_3903_cov_10.852651, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000243 DAOQOD010000000
VERSION     DAOQOD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_3903_cov_10.852651"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLZ72_15240"
     CDS             <1..1373
                     /locus_tag="PLZ72_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37702.1"
                     /translation="DNSFLTHLATGRLILEQGVPSTNPFLYSARIYPGSAEFPIPSWL
                     WSVVLGGVDAVAGGAGLRVLTAALAGVLGLVVVRLTRPVTGGERTLLSVVLPATCVVI
                     TLMPFVNARPQLPGYLFLALTVLVATERRSGWWLVAVFALWANVHGTWFYGLAVLGLL
                     VVAQVLDERRIGTRRLGWLVGAVVGVVLGGLLQPHPFETVILPLEQLGDERARRAIAN
                     YVEWKPAGFDHPLTWLLIAMGLVALFAALGLRQGPDGSDGPRRWGVLLGAVGLVAMGM
                     SAGRLLPLAVITLVPWVAMGLQGLRGLPLPSGGIPRVLASLGVLLGVVALVWSTSNPA
                     YDLSRYPVTAIDWLAERGLMGSADVKVASHDYVGNYLDWRYEDRANTFVDDRPGTDAL
                     LDYAALQDLTDGWRDALGRAEPDVIVWETERPLTDELREPAWYDAGRFGEFTVFCRSS
                     IADRCR"
     gene            complement(1330..2256)
                     /locus_tag="PLZ72_15245"
     CDS             complement(1330..2256)
                     /locus_tag="PLZ72_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA37703.1"
                     /translation="MNIDDLRPGRLVARLGFPFTAAPTPPGVEPPVEPNRLGDRYDTD
                     WARRPLARATRQAVIRTVGRVGTAALARPSVAGLDRLDGLDSNVIFVANHHSHVDTPV
                     LLTHIPRPWRDELVVAAAADHFYDTRPKAALISWAYGAIPMERKKVSRRSADTAAGLL
                     DDGWSLLIFPEGGRSPDGWGQPHKGGAAYLAVRVGVPVVPVHLDGTGLILPKGSNTPR
                     PGKVRVNFGAPLRPSDGEDARRFAIRLEAAVSELADESSGDWFSARLRAHAGDTPGLH
                     GPDDSWIRDWRSPDRRPQITDSDRRWSSGRTR"
     gene            complement(2267..3808)
                     /locus_tag="PLZ72_15250"
     CDS             complement(2267..3808)
                     /locus_tag="PLZ72_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNA37704.1"
                     /translation="MLFHHGEQLRLEKLPAERSRIIYPPEPLAGLDDPDQAIRDALEN
                     PIDSEPLSALLFADMRLTIAFDDISLPLPQMRRPDIRQRVIEAVLDLAAEAGVTDVHL
                     IAALALHRRMTEEELRHAVGDRVYDSFAPRGQLYNHDAEDPDGMTLIGTTRHGEEVQI
                     STRAATSDLIVYVNINLVSMDGGWKSTATGLSGYQALRHHHNVHTMRQSRSFMDRESS
                     ELHRSNWRQGEVLRDAGIKVFQIETTVNNDLYGDGPLKVLAKREWEWTTRDRATFMAA
                     KAGLDRLSPAQRRRILHGWRAPYAMTSVQAGEVEAVHEVTTRHVYDQQLVPVEGQTDI
                     LALGLPYICPYNVNSIMNPILVMCLGLGYFFNLYKGRPLVREGGVLIMSHPTTPEFHP
                     VHHPSYIDFFEEVLAETTDPLVMEAQFEERFATDEWYRHLYRTSHAYHGVHPFYMWYW
                     GSHALEHLGGVVVVGGDPATVRRLGFRPASTMSDALEIASDVVGRQPTITYFKNPPLS
                     MVDVT"
BASE COUNT          508 a         1309 c         1453 g          633 t
ORIGIN      
        1 ccgacaacag cttcctgacc cacctggcga ccggccggct gatcctggaa cagggggtgc
       61 cgagcaccaa cccgttcctg tactcggccc gcatctatcc gggatcggcc gagttcccga
      121 tcccgagctg gctctggtcg gtggtcctcg gcggtgtcga cgcggtcgcc ggcggtgccg
      181 gactccgggt gctgaccgcg gccctggccg gcgtgctggg cctggtcgtg gtgcgcctca
      241 cccgaccggt gacaggaggg gagcggactc tgctgtcggt cgtgctgccg gcgacgtgcg
      301 tggtgatcac gctcatgccg ttcgtgaacg cccgaccgca gctgcccggg tacctgttcc
      361 tggcgctgac ggtgctcgtc gccaccgagc gacggtccgg ctggtggctc gtggcggtat
      421 tcgccctctg ggccaacgtc cacggcacct ggttctacgg tctcgccgtg ctggggctgc
      481 tcgtcgtcgc ccaggtgctc gacgaacgcc ggatcgggac ccgacgactc ggctggctcg
      541 tcggtgccgt cgttggagtg gttctgggtg gactcctcca gccgcacccg ttcgagaccg
      601 tgatcctgcc gctcgagcag ttgggggacg agcgggcccg acgggcgatc gccaactacg
      661 tcgagtggaa gcccgccggc ttcgaccacc cgctcacctg gctgctgatc gccatggggc
      721 tggtcgccct gttcgcagca ctcggtctcc gccagggtcc cgacggatcc gacggtccgc
      781 gtcgatgggg ggtgctcctc ggtgcggtcg gcctggtcgc gatgggcatg tcggccggcc
      841 ggctcctacc cctggcggtg atcacgctcg tgccctgggt ggcgatgggt ctccaggggc
      901 ttcggggcct gccgctgccg tccggtggca tccctcgggt cctggcctcg ctcggggtgc
      961 ttctcggggt ggtcgcgctg gtctggagca cgtcgaaccc ggcctatgac ctgtcccgct
     1021 acccggtcac ggccatcgac tggctcgccg agcgggggct gatgggcagc gcggatgtca
     1081 aggtcgccag ccacgactac gtcggcaact acctggattg gcgctacgag gatcgggcca
     1141 acaccttcgt cgacgaccga cccggcaccg acgccctgct ggactacgcc gcgctgcagg
     1201 acctgaccga cgggtggcgt gacgccctcg ggcgcgctga gccggacgtg atcgtgtggg
     1261 agaccgagcg gccgttgacc gacgagctgc gtgaaccggc ctggtacgac gcgggtcggt
     1321 tcggcgagtt caccgtgttc tgccggagct ccatcgccga tcgctgtcgg tgatctgcgg
     1381 gcgacggtcc ggggagcgcc agtcgcggat ccagctgtcg tccggtccat gcagcccggg
     1441 ggtgtcgccc gcgtgcgccc gcagtcgggc cgagaaccag tcgccgctcg actcgtcggc
     1501 cagctccgat acggcggcct ccagccggat ggcgaagcga cgggcgtcct cgccgtcgga
     1561 gggccgcagc ggcgccccga agttgacgcg caccttcccc ggacggggcg tgttggaccc
     1621 cttgggcagg atcaggccgg ttccgtcgag gtgcaccggc accaccggca cgccgacccg
     1681 gaccgccagg taggccgctc cgcccttgtg gggttggccc cagccgtcgg ggctgcggcc
     1741 gccctcgggg aagatgagca gcgaccagcc gtcgtcgagc aggcccgccg cggtgtccgc
     1801 ggagcggcgg ctgaccttct ttcgctccat cgggatcgcg ccgtacgccc acgagatgag
     1861 cgctgccttg gggcgggtgt cgtagaagtg gtcggcggcg gcggcgacca ccagctcgtc
     1921 gcgccacggg cgcgggatgt gggtgaggag caccggggtg tcgacgtggc tgtggtggtt
     1981 ggccacgaag atcacgttcg agtccaaccc gtcgaggcgg tcgagtccgg cgacgctcgg
     2041 gcgcgccagc gccgcggtgc cgacccggcc gacggtgcgg atgacggcct ggcgggtggc
     2101 gcgggccagc ggccggcgcg cccagtcggt gtcgtagcgg tcgcccaggc ggttcggctc
     2161 caccggcggc tccacccccg gcggggtggg agcggcggtg aacgggaagc ccagtcgggc
     2221 caccagccgt cccgggcgca ggtcgtcgat gttcaccggt gcaccgtcac gtcacgtcga
     2281 ccatcgacag cggcgggttc ttgaagtagg tgatcgtcgg ctgccgaccg accacgtccg
     2341 acgcgatctc gagggcgtcc gacatggtcg acgccggccg gaagcccagc cgacgcacgg
     2401 tggccggatc gccgccgacg acgaccacgc cgccgaggtg ctccagggcg tggctgcccc
     2461 agtaccacat gtagaacggg tgcacgccgt ggtaggcgtg gctggtgcgg tacaggtgcc
     2521 ggtaccactc gtcggtggcg aagcgctcct cgaactgcgc ctccatcacc agggggtcgg
     2581 tcgtctcggc gagcacctct tcgaagaagt cgatgtagct cgggtggtgc accgggtgga
     2641 actcgggcgt ggtcgggtgg ctcatgatca gcaccccgcc ttcgcgcacc agcggccgac
     2701 ccttgtagag gttgaagaag tacccgagac ccaggcacat gaccaggatc gggttcatga
     2761 tcgagttcac gttgtagggg cagatgtagg gcaggcccaa cgccaggatg tcggtctggc
     2821 cctcgacggg caccagctgc tggtcgtaca cgtgacgggt cgtcacctcg tggacggcct
     2881 ccacctcgcc ggcctgcacc gacgtcatgg cgtacggcgc ccgccacccg tgcaggatcc
     2941 tgcgtcgctg ggcgggtgac aggcggtcga ggccggcctt ggcggccatg aacgtggccc
     3001 ggtcgcgggt ggtccactcc cactcgcgct tggccagcac cttcagcggc ccgtcgccgt
     3061 agaggtcgtt gttgaccgtg gtctcgatct ggaacacctt gatgcccgcg tcgcgcagca
     3121 cctcgccctg gcgccagttg ctcctgtgca gctcggagct ctcgcggtcc atgaagctgc
     3181 gcgactggcg catcgtgtgc acgttgtggt ggtggcgcag cgcctggtag cccgacaggc
     3241 cggtcgcggt cgacttccag ccgccgtcca tcgacaccag gttgatgttg acgtagacga
     3301 tcaggtccga cgtcgcggcc cgggtggaga tctgcacctc ctcgccgtgg cgcgtggtgc
     3361 cgatcagcgt catcccgtcg gggtcctcgg catcgtggtt gtagagctga ccccgcggcg
     3421 cgaacgagtc gtagacccgg tccccgacgg cgtggcgcag ctcctcctcg gtcatccggc
     3481 ggtgcagcgc gagcgcggcg atcaggtgga cgtcggttac accggcctcg gcggccaggt
     3541 cgagcacggc ctcgatcacc cgctgacgaa tgtcgggtcg gcgcatctgc ggaagcggca
     3601 gcgagatgtc gtcgaaggcg atcgtcaggc gcatgtcggc gaacagcaga gccgagagcg
     3661 gctcggagtc gatcgggttc tccagcgcgt cgcggatcgc ctggtcggga tcgtcgagac
     3721 ccgccagggg ctcgggcgga tagatgatcc tgctgcgctc ggccgggagc ttctcgagtc
     3781 ggagctgctc gccgtggtgg aacagcaccg ggggagtgga gcggtcgacg tcgaggacca
     3841 gtccgggacg gggagccggg cgtgagctgc cgcgggtcgg acgtcgggga cgggaggagg
     3901 ggg
//
LOCUS       DAOQOD010000244         3899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_10765_length_3899_cov_10.232570, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000244 DAOQOD010000000
VERSION     DAOQOD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10765_length_3899_cov_10.232570"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            764..1123
                     /locus_tag="PLZ72_15255"
     CDS             764..1123
                     /locus_tag="PLZ72_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37705.1"
                     /translation="MGRLDARSLAQATAALRVTSTSLAAGEVVHRLVVGHAKGLVCVL
                     ARTDRRLLVVADRPGRALVESLHPHQTMVGVQDADDGTVTVVVSDGRRVMRLEGVHEV
                     AEAELLVGPDRSGPTYF"
     gene            complement(1187..>2003)
                     /locus_tag="PLZ72_15260"
     CDS             complement(1187..>2003)
                     /locus_tag="PLZ72_15260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNA37706.1"
                     /translation="VAEVVDSHVHLLPGRLGAKVRTIFTDVGGYDLAYPADHAAVCEQ
                     LAAEGVAAVWTLPYAHRPGVADSLNRASTATAAADLAVQVVAGATVHPGDDAPVTLVR
                     RAVEDDGARVLKLHCSVGRFDPLDPALAPVWDYVEETRLPVVVHAGRSPDGVGSGDDL
                     LPLDETARRHPAARIVIAHCGHDHETDAVAILDRHPNVHADLTPVVNRLVQVPAADAA
                     RLADRLLFGTDAPNTEVTAARCRAHVEALGLVPEATSAILGGNARRLVAEVAD"
     assembly_gap    2004..2103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2104..>3899)
                     /locus_tag="PLZ72_15265"
     CDS             complement(<2104..>3899)
                     /locus_tag="PLZ72_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNA37707.1"
                     /translation="DLKAAGVVVRHDTYEHNYPHCWRTDTPIIYKAVSSWYVEVTAFR
                     DRLVELNQDINWIPSHVRDGQFGKWLEGARDWSISRNRFWGSPIPVWRSDDPAYPRLD
                     VYGSLAEIEADFGVRLDDLHRPTVDELVRPNPDDPTGRSMMRRVPEVFDCWFESGSMP
                     FAQVHYPFEHREWFEEHSPADFIVEYIAQTRGWFYTLHVLSVALFDRPAFSNVICHGV
                     VLDHEGRKLSKKLRNYPDPEEVMTTLGSDALRWYLMASPILRGGDLRIATDASDISEV
                     VRLVINPIWNAYAFFTLYANADGERGRFRTDATGVLDRYVLAKTRELVERVGDRMDAF
                     DIAGSCQEVLAFLDALNNWYIRRSRERFWGSSAEVTAQDRADARDTLFTVLSTLVRVT
                     APLLPFVSDEIHLGLHPEADGTSSVHLEDWPDAASLPSDPELVDAMDRIREVCSVALG
                     LREDHRLRARLPLRSLTVAGRHLDGLEPLAALVRDEVNVKDVVFTDDVESVGSFQLRP
                     NARVLGPRLGKDVQSVIRAAKAGEWAAGGDGTVTVAGHELGADEFDLTLEARDGAAAA
                     PLHDSSAVVELDVEVTPELAAEGLARDAVRMI"
BASE COUNT          493 a         1328 c         1447 g          531 t
ORIGIN      
        1 gccgcctggc ccgcgctctc cccggacggc accgctgccc ctgcctccgc ctccgtcccg
       61 acccgagccg gtggcaccgg cggctccgtg gccgcccgtt gccgcccctg cgccgccggc
      121 gccgcccgcg gtcgaacgga cggtcgcgcc gtcgcctccg gtcgttcccg ttcccccggg
      181 cgctgttccc ccgagcgtgc cgccgccgtc gtccgaaccc gagccgctcg tcgcggagag
      241 cgggtccgac gtgggtgccg accgaccgtc gcgacgccgg gtccggccga cgggtcggtg
      301 gagcgcggcg gggaccaccc gtcgacgccg ctcccgcacc gaggccgacc gcgctgcggg
      361 ttggaccgcg tctccggccg aacctccgga gccgatcgag cctcccgaga cggtcgagtc
      421 ggcaggtccc accgtgggtg tcgacggtgg ctcgaccgga caccacgtcg gtccgggacc
      481 tcaggaccgg tggacggtcc cggacacgcc tgcggcccga tcgaccttcg ggaccggacc
      541 ggagccccgg gtcccgtccg cgtcgcgctt cagcgtcggc agccgctcga ccggagcgtc
      601 agccggtgac agcggcgtcc cgtccggtgg gtccggaccg ttcgggcgcg gaacgccccg
      661 acgggacgcc gggggcggtc cgggccgctc cggcggctcg ggcggctcag gcttcggctc
      721 gggttctgcc ggcgatggtc ggtccgaccc gatggccgtg gcgatgggac ggctcgacgc
      781 gaggtcgctg gcccaggcga cggcggcgct gcgggtgacc tcgacgtcgt tggctgccgg
      841 ggaggtcgtg catcgtctgg tggtgggcca cgccaagggg ctggtctgcg tcctggcccg
      901 caccgatcgt cgactgctcg tggtcgcgga ccgacccggt cgtgcgctgg tcgagtcgct
      961 gcacccccac cagacgatgg tgggagttca ggacgcggac gacgggacgg tgaccgtcgt
     1021 ggtctccgac ggccgtcgcg tcatgcgcct ggagggggtg cacgaggtgg ccgaagccga
     1081 gttgctcgtc ggtccggacc ggtcggggcc gacgtacttc tgactggagt gcgggccagc
     1141 cggtcagcca gctcagccgg tcagccagct cagtcggcca gccagctcag tccgcgacct
     1201 cggcgacgag gcgacgggcg ttgccgccca ggatcgcgga ggtcgcctcc gggaccaggc
     1261 cgagcgcctc gacgtgggcc cggcaccgcg ccgcggtcac ctcggtgttg ggcgcgtcgg
     1321 tcccgaacag caggcggtcg gccaggcggg ccgcgtcagc cgccggtacc tggaccagcc
     1381 ggttgaccac cggggtgagg tcggcgtgca cgttcgggtg acgatcgagg atcgccactg
     1441 cgtcggtctc gtggtcgtgc ccgcagtgag cgatcacgat ccgcgccgcc gggtgtcgac
     1501 gtgccgtctc gtcgagcggg agcaggtcgt cgccggagcc gaccccgtcg ggtgagcggc
     1561 cggcgtgcac caccaccggc agccgggtct cctcgacgta gtcccacacc ggcgcgagtg
     1621 ccggatcgag cggatcgaag cgaccgaccg agcagtggag cttgaggacc cgggctccgt
     1681 cgtcctcgac cgcccggcgc acgagcgtca ccggtgcgtc gtcgcccgga tgcaccgttg
     1741 cgccggcgac gacctgcacc gccaggtcgg ccgccgcggt ggcggtcgac gcccggttga
     1801 gcgaatcggc gacacccggt cgatgggcgt acggaagggt ccagaccgcc gccacgccct
     1861 cggccgccaa ctgctcgcac accgcggcgt ggtccgccgg gtacgccagg tcgtagccgc
     1921 cgacgtcggt gaagatcgtc ctcaccttgg cgcccagccg gccgggcagc aggtggacgt
     1981 ggctgtcgac gacctcggcg acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnntggatca tgcgcaccgc gtcgcgggcc agtccctcgg cggcgagctc cggggtgacc
     2161 tcgacgtcga gctcgaccac ggcgctgctg tcgtgcaggg gagcggcggc ggcgccgtcg
     2221 cgggcctcga gggtgaggtc gaactcgtcc gcgcccagct cgtgtccggc cacggtcacg
     2281 gtgccgtcgc cgccggcggc ccactcgccg gccttggccg cccggatgac gctctggacg
     2341 tccttgccga gccggggtcc gaggacccgg gcgttcggtc gcagctggaa cgagccgacg
     2401 gactccacgt cgtcggtgaa cacgacgtcc ttcacgttga cctcgtcccg gacgagcgcg
     2461 gccaggggct ccaggccgtc gaggtggcgt ccggcgacgg tcaggctccg cagcggcagg
     2521 cgggcccgca ggcggtggtc ctcccgcagc ccgagcgcga ccgagcacac ctcgcggatg
     2581 cggtccatcg cgtcgaccag ctccgggtcg gagggcagcg aggcggcgtc gggccagtcc
     2641 tcgaggtgca ccgaggacgt gccgtcggcc tcggggtgca gccccaggtg gatctcgtcg
     2701 ctcacgaacg gcagcagcgg cgcggtgacc cgcaccaggg tgctgagcac cgtgaacagc
     2761 gtgtcgcggg catcggcgcg gtcctgggcg gtcacctcgg cgctggaacc ccagaaccgc
     2821 tcgcgggacc ggcggatgta ccagttgttg agcgcgtcga ggaaggcgag gacctcctgg
     2881 caggaaccgg cgatgtcgaa ggcgtccatg cggtcgccga cgcgttcgac cagctcgcgg
     2941 gtcttggcca ggacgtagcg gtcgagcacg ccggtcgcgt cggtgcggaa ccggccccgc
     3001 tcgccgtccg cgttggcgta cagggtgaag aacgcgtacg cgttccagat cgggttgatg
     3061 accaggcgca ccacctcgct gatgtcggag gcgtcggtcg cgatgcgcag gtcgccgccg
     3121 cgcaggatcg gcgacgccat caggtaccag cggagcgcgt cggagccgag ggtggtcatc
     3181 acctcttcgg ggtccgggta gttgcgcagc ttcttggaga gcttgcggcc ctcgtggtcc
     3241 agcacgactc cgtggcagat gacgttggag aacgccggtc gatcgaacag tgccaccgac
     3301 aggacgtgca gcgtgtagaa ccagccgcgg gtctgggcga tgtactcgac gatgaagtcc
     3361 gcgggggagt gctcctcgaa ccactcgcgg tgctcgaacg ggtagtgcac ctgggcgaag
     3421 ggcatggaac ccgactcgaa ccagcagtcg aacacctcgg ggacccgccg catcatcgac
     3481 cggccggtcg ggtcgtcggg gttgggccgg accagctcgt cgacggtcgg gcggtgcagg
     3541 tcgtcgaggc ggacgccgaa gtcggcctcg atctcggcca ggctgccgta gacgtcgagg
     3601 cgcgggtacg cggggtcgtc ggagcgccac accgggatgg gtgaacccca gaagcggttg
     3661 cggctgatcg accagtcgcg ggcgccctcg agccacttgc cgaactggcc gtcacgcacg
     3721 tgggacggga tccagttgat gtcctggttc agctcgacga gccggtcgcg gaaggcggtg
     3781 acctcgacgt accacgacga cacggccttg tagatgatcg gggtatcggt gcgccagcag
     3841 tgcgggtagt tgtgctcgta ggtgtcgtgg cggacgacga cgccggcggc cttgaggtc
//
LOCUS       DAOQOD010000245         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11075_length_3840_cov_10.587318, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000245 DAOQOD010000000
VERSION     DAOQOD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11075_length_3840_cov_10.587318"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..911
                     /gene="uppS"
                     /locus_tag="PLZ72_15270"
     CDS             192..911
                     /gene="uppS"
                     /locus_tag="PLZ72_15270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNA37708.1"
                     /translation="MELSALDPGRIPVHVAIVMDGNGRWAQKRGLPRTDGHAAGEEAL
                     LDTVWGALDAGIRYLTVYAFSTENWKRPVDEVRFLMGFNERLLMTRRDELHAKGVRVQ
                     FLGRRDWRVPKRVLRRITETEEMTRHNTTMTLSMAFNYGGRAELVDAVRAIARSGVKP
                     DKIDEKLIRRHLYDPDQPDPELMIRTSGEHRISNFLLWEIAYSELVFTEVLWPDFRRG
                     DLYEAIDEFQRRERRFGGLNA"
     gene            922..1641
                     /gene="recO"
                     /locus_tag="PLZ72_15275"
     CDS             922..1641
                     /gene="recO"
                     /locus_tag="PLZ72_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNA37709.1"
                     /translation="MSLYRDSAVVLRTHKLGEADRIVVLMTRESGKVRAVAKGVRRTG
                     SKFGSRLEPGSYAALQLHEGRGELDIVTQAETIESFRRTREDLSRLGRAASLLEAVEQ
                     LSEDRQPAPRLHDMLVGGLRTVDERNPPLVTGAFFLKLLAAEGVAPELDRCVGCGEED
                     GELALALDVGGVRCRSCGGGRPVTDEALAVARAVLGGGLNVALSLPDGSLTHEVESIA
                     TTALELHVERRLRSLQVLHDW"
     gene            1724..3088
                     /locus_tag="PLZ72_15280"
     CDS             1724..3088
                     /locus_tag="PLZ72_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNA37710.1"
                     /translation="MAEQSRYLLNDDQVPTSWYNIVPDLPAPPPPPLHPATHQPIGPD
                     DLAPLFPMDLILQEVSQDRYVPIPDEVRDVYLLWRATPLHRAHRLERALGTPARIYYK
                     YEGTSPAGSHKPNTAVPQAYYNAKAGIRRLTTETGAGQWGTALSFAAAQYGIDCEIWQ
                     VRASYDQKPYRRAMMEAFGGTVHPSPSDVTEYGRKMLAEDPTNPGSLGIAITEAVEVA
                     AQDPDSRYALGSVLNHVLMHQTVIGEEALLQMAMAGDVPDVIIGCTGGGSNFAGLTFP
                     FLREKLAGTMDPEIRAVEPASCPTLTKGTYAYDFGDTGGMTPLVKMHTLGHDFIPDPI
                     HAGGLRYHGMAPLISHIYELGLITAESRLQRECFAAGVQFARTEGVVPGPEPTHALAS
                     TVDEALRCKETGEEKVILTALSGHGMLDMAAYDAYFAGELVDHEFSDELMAEALTRLP
                     QLDL"
     gene            complement(3202..>3840)
                     /locus_tag="PLZ72_15285"
     CDS             complement(3202..>3840)
                     /locus_tag="PLZ72_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37711.1"
                     /translation="GASISYGWKKFQENAGPFVILMLIVFGATLVVSLLGQLVLLPLF
                     RGDSGGSAIMSMIGFSVVFVLQFVVAFFLQAGVYRAGLGVTRGIKPEVSMLTDTTHMA
                     NFALTVVLVGLGAFVGYLLCFIPGIIWLIFTAYAPIIALDKGVGPGDAIKQSIDWVKD
                     NFGKVFLILLVAYLVYYVGVLLCCIGVLATGPIALISIIHSYRALNQEPVVP"
BASE COUNT          627 a         1381 c         1312 g          520 t
ORIGIN      
        1 tcttgaagag cgcccggcgc gcgatcagcg acacccgggc gccccgggcg atcgcggcgg
       61 cggccgtggc ccgtccgatg ccctccgatc caccggtgat gatcacgtgg gcgtcgcgca
      121 gcgaggtcat gtcggcattc tgcccaccgc gacgcccgtc gggcgtgggg gacggcacta
      181 gcgtggccac ggtggagctg agcgcactcg atccgggccg gatccccgtc cacgtggcga
      241 tcgtcatgga cggcaacggg cggtgggccc agaagcgggg gctgccccgc accgacgggc
      301 acgccgccgg cgaagaggcg ctgctcgaca cggtgtgggg agcgctcgat gccgggatcc
      361 gctacctgac cgtgtacgcg ttctccaccg agaactggaa gcgccccgtc gacgaggtcc
      421 gattcctgat ggggttcaac gaacggctcc tcatgacccg gcgcgacgag ctgcacgcca
      481 agggggtccg ggtgcagttt ctgggtcgcc gcgactggcg ggttccaaag cgggtgctgc
      541 ggcgcatcac cgagaccgag gagatgaccc gccacaacac gacgatgacg ctgtcgatgg
      601 cgttcaacta cggcggccgg gccgagctgg tcgacgcggt ccgggccatc gcccgctcgg
      661 gggtcaagcc cgacaagatc gacgagaagc tcatccgccg ccacctctac gaccccgacc
      721 agccggaccc ggagctgatg atccgcacct ccggcgagca ccgcatctcc aacttcctgc
      781 tgtgggagat cgcctactcg gagctggtgt tcaccgaggt gctgtggccg gacttccgac
      841 gcggcgacct gtacgaggcg atcgacgagt tccaacgccg cgagcgccgc ttcggcggac
      901 tcaacgcatg aggccgaccg ggtgagcctc taccgcgact cggcggtggt gctgcgcacc
      961 cacaagctcg gcgaagccga tcgcatcgtg gtgctgatga cccgggagtc gggcaaggtc
     1021 cgcgccgtcg ccaagggggt gcgccgcacc ggctcgaagt tcgggtcccg gctggagccc
     1081 ggctcctacg cggcgttgca gctgcacgag gggcgcggcg agctcgacat cgtcacccag
     1141 gccgagacca tcgagtcgtt ccgtcgcacc cgtgaggacc tgtcccgcct gggtcgggcc
     1201 gcgtcgctgc tcgaagcggt cgagcagctc tccgaggacc gccaacccgc accgcggctc
     1261 cacgacatgc tcgtgggcgg tctgcgcacc gtcgacgagc gcaacccgcc gctggtgacg
     1321 ggagcgttct tcctcaagct gctggccgca gagggcgtcg cccccgagct cgaccgctgc
     1381 gtcggctgcg gcgaggagga cggcgagctg gcgctggccc tcgacgtcgg cggggtgcgt
     1441 tgccggtcct gcggaggagg tcggccggtg accgacgagg cgctcgcggt ggcccgggcc
     1501 gtgctgggcg gcgggctcaa cgtggccctg tcgctgccgg acgggtcgct cacccacgag
     1561 gtggagtcga tcgccaccac cgccctcgag ctgcacgtcg aacgccgcct ccggtcgctg
     1621 caggtgttgc acgactggtg agccccggcc gatcatcgcc gcagcacgat ccgaatgccc
     1681 caccgagacc accccacccg agaccacgga gaacggagtc cccatggctg agcagagccg
     1741 ctacctgttg aacgacgacc aggtgccgac ctcctggtac aacatcgtcc ccgacctgcc
     1801 ggcaccgccg ccgccgccgc tgcacccggc gacgcaccag ccgatcggcc ccgacgacct
     1861 ggccccgctg ttcccgatgg acctgatcct ccaggaggtc agccaggacc gctacgtgcc
     1921 gatccccgac gaggtccgcg acgtgtacct gctgtggcgg gccaccccgt tgcaccgggc
     1981 ccaccggctg gagcgggcgc tcggcacgcc ggcgcgcatc tactacaagt acgagggcac
     2041 cagccccgcc ggctctcaca agcccaacac cgcggtgccc caggcgtact acaacgccaa
     2101 ggccgggatc cggcggctga ccaccgagac cggggccggt cagtggggta cggcgctcag
     2161 cttcgccgcc gcccagtacg gcatcgactg cgagatctgg caggtccggg cctcctacga
     2221 ccagaagccg taccgccggg cgatgatgga ggccttcggc ggcaccgtgc acccgtcgcc
     2281 gtccgacgtc accgagtacg gccggaagat gctggccgag gacccgacca acccggggtc
     2341 gctcggcatc gccatcaccg aggcggtcga ggtggccgcc caggaccccg acagccgcta
     2401 cgcgctcggc tcggtgctca accacgtgct gatgcaccag accgtgatcg gcgaggaggc
     2461 cctgctgcag atggcgatgg ccggcgacgt ccccgacgtc atcatcggct gcaccggcgg
     2521 cggttccaac ttcgccgggc tcaccttccc gttcctgcgc gagaagctcg ccgggaccat
     2581 ggatcccgag atccgcgccg tcgagcccgc cagctgcccc acgctgacga agggcaccta
     2641 cgcgtacgac ttcggcgaca ccggcggcat gacgccgctg gtcaagatgc acaccctggg
     2701 ccacgacttc atcccggacc cgatccacgc cggcgggctg cgctaccacg gcatggcccc
     2761 gctgatctcg cacatctacg agctgggtct gatcaccgcc gagtcacggc tccagcggga
     2821 gtgcttcgcg gcgggcgtgc agttcgcccg caccgagggc gtcgtacccg gtccggagcc
     2881 cacgcatgcc ctggcgtcga cggtcgacga ggcgttgcgc tgcaaggaga ccggcgagga
     2941 gaaggtcatc ctcaccgcgc tcagcggcca cggcatgttg gacatggcgg cctacgacgc
     3001 ctacttcgcc ggtgagctcg tcgaccacga gttctccgac gagctgatgg ccgaggcgct
     3061 cacccgcctc ccgcagctcg acctctgaac cctttcccgg gggatgtccg accccggggc
     3121 cggacgcgac gagggggccc cgctcggggc cccctcgtcc gcgtgccggg acggttcgcc
     3181 cggagccggt cgcggtcccg gctacgggac caccggctcc tggttgagcg cccggtagga
     3241 gtggatgatc gagatcagcg cgatcgggcc ggtggccagc acgccgatgc agcacagcag
     3301 gacgccgacg tagtacacga ggtaggcgac gagcaggatc aggaacacct tgccgaagtt
     3361 gtccttgacc cagtcgatgc tctgcttgat cgcgtcgccg ggacccacgc ccttgtccag
     3421 ggcgatgatc ggggcgtacg cggtgaagat cagccagatg atgccgggga tgaagcacag
     3481 caggtagccg acgaaggcgc cgagcccgac gagcacgacc gtcagcgcga agttcgccat
     3541 gtgcgtcgtg tcggtcagca tcgacacctc gggcttgatg ccccgggtca cgccgagccc
     3601 ggcgcggtag acgccggcct ggaggaagaa cgccacgacg aactgcagga cgaacaccac
     3661 agagaagccg atcatgctca tgatggcgct gccgccgctg tcaccccgga acaggggcag
     3721 cagcaccagc tggccgagca acgacaccac gagcgtcgca ccgaacacga tcagcatcag
     3781 gatgacgaac gggccggcgt tctcctggaa cttcttccag ccgtaggaga tcgatgcacc
//
LOCUS       DAOQOD010000246         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11232_length_3809_cov_11.225360, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000246 DAOQOD010000000
VERSION     DAOQOD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11232_length_3809_cov_11.225360"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1416
                     /gene="manB"
                     /locus_tag="PLZ72_15290"
     CDS             43..1416
                     /gene="manB"
                     /locus_tag="PLZ72_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442410.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNA37712.1"
                     /translation="MPSLDAIFKAYDVRGTVPDQLDPELAHAIGVGFATFAREESEGR
                     GESVTRVLMARDMRPSGVELSAAFADGVRSQGLDVVDLGLGSTDLLYFASGSMGAPGV
                     MFTASHNPAQYNGAKFCLSGARPVGEDTGLGRIREVGEASLAGSVPAAATTGTISSLD
                     VLGAFADHVRSFVDVSSLAPLQIVADTANGMGGLIVPAVFSSLPFQLEVMYGELDGTF
                     PNHPADPIQPANTEDLRRRVVEAGADVGLAFDGDADRVFLVDEKGVGLSGSTTTAIIA
                     AGILDKNPGGTVIHNLICSRTVPEVVREHGGTPVRTRVGHSFIKQVMAEEGAVFGGEH
                     SAHYYFRDNFRADSGIIAALLVLEQMSVAGTSLSELRAPFERYADSGEINTRVDDPAA
                     VIERVAEHFSDLPQDRLDGLTVDGGDWWFNLRPSNTEPLLRLNLEAPDAEACTRRTEE
                     VRALLTA"
     gene            1413..1691
                     /locus_tag="PLZ72_15295"
     CDS             1413..1691
                     /locus_tag="PLZ72_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNA37713.1"
                     /translation="MTSPSKETTTMTTDQTLDPRLLEILACPEDKGPLLYFADEQSLY
                     NPRLHRRYDVRDGIPVMLIDEASSVDDAEHDRLMAKAGADGIEPTFSE"
     gene            1688..2758
                     /locus_tag="PLZ72_15300"
     CDS             1688..2758
                     /locus_tag="PLZ72_15300"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNA37714.1"
                     /translation="MSAPVDGAGTQGVGSVLDSLGMFAEAYALPEQMAAARVSAASAD
                     GLPDAAGVTSVVILGVGGSGIGGDICAAVAGPDCPVPVVVSKHYECPGFVGPDTLVFA
                     VSFSGNTEEAIGAATAARDAGARLVVVAAGGELARLAGEWGVPLLPVDDSIPMPRAGI
                     GAVTVPLLVALERMGLTDGMTGQIDAAIAQLERRREQLRVASGPAERLAARIGGTLPL
                     VYGGGALGEVAAWRWKGQFNENPKVPAFCNRIPELTHNELAGWAQHGDVTRQVLTMVH
                     LRHDFEHPNVSRRFDFVASVCDEVVADIHTVRAEGDGRLAQLMDLVLQGDLVTLHLAA
                     RAGVDPGPIAVLDDLKEWLRNA"
     gene            2815..3780
                     /locus_tag="PLZ72_15305"
     CDS             2815..3780
                     /locus_tag="PLZ72_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA37715.1"
                     /translation="MLGADSTTDDVLAGVDLTGRTAVITGSSGGLGLETARALAARGA
                     AVVLAARDRVKLDAAVAQLRDQVPGITVDSVLLDLADLTSVRAAAAEVADRHPVIDLL
                     IDNAGVMACPLGRTTDGFETQFGTNHLGHFLFTTLLKEPLATAVAEHGSARVVVLSSG
                     GHRLSPIRWDDPNWERSEDYFNWTAYGQSKTANALFALELDRRWSSDGIRAFSVHPGM
                     IATDLGRHMQAEDFEYLSSVRRTGADSADGGRAVFKSVPQGAATTVWAATSPALEAHG
                     GAYLENCSVAEPVDDPDATHGVRSWARDPDQAVRLWELSEDLVGA"
BASE COUNT          524 a         1374 c         1350 g          561 t
ORIGIN      
        1 cggactccga agtgtcgcgt cccggccact agggtgcgcc ccatgccgtc cctcgacgcc
       61 atcttcaagg cctacgacgt ccgcggcacg gttcccgacc agctggaccc cgagctcgcc
      121 cacgccatcg gcgtcggctt cgccaccttc gcccgtgaag agtccgaggg tcgcggcgag
      181 tcggtcaccc gggtgctgat ggcccgcgac atgcgcccgt ccggcgtgga gctgtccgcc
      241 gcgttcgctg acggggtgcg atcccagggc ctcgacgtgg tcgacctcgg gctcggctcg
      301 accgacctcc tctacttcgc ctcgggctcg atgggcgccc ccggggtcat gttcaccgcg
      361 tcgcacaacc cggcccagta caacggtgcc aagttctgcc tgtcgggggc ccggccggtg
      421 ggcgaggaca cggggctcgg ccgcatccgc gaggtcggcg aggcgtctct ggcgggatcg
      481 gttccagctg cggcgacgac cgggaccatc agctccctcg acgtgctcgg cgcattcgcg
      541 gatcacgtcc gctcgttcgt cgacgtgtcg tcgttggctc cgctgcagat cgtggccgac
      601 accgccaacg gcatgggtgg cctgatcgtc ccggcggtgt tctcctcgct gccgttccag
      661 ctcgaggtca tgtacggcga gctcgacggg accttcccca accatcccgc ggatccgatc
      721 cagccggcca acaccgagga cctgcgccgc cgggtcgtcg aggccggcgc ggacgtcggc
      781 ctggccttcg acggcgacgc ggaccgggtg ttcctggtcg acgagaaggg cgtcggcctc
      841 tcgggatcga ccaccacggc catcatcgcc gccgggatcc tcgacaagaa ccccggcggc
      901 accgtgatcc acaacctgat ctgctcgcgg accgtccccg aggtggtgcg cgagcacggg
      961 ggaacgccgg tgcggacacg ggtcggccac tcgttcatca agcaggtcat ggccgaggag
     1021 ggcgccgtct tcggcggcga gcactccgcg cactactact tccgcgacaa cttccgcgcc
     1081 gattcgggca tcatcgccgc cctgctggtg ctcgagcaga tgtcggtggc cggaaccagc
     1141 ctgtcggagc tgcgtgcacc gttcgagcgc tacgccgact ccggagagat caacacccgc
     1201 gtcgacgacc ccgccgcggt gatcgagagg gtggcggagc atttctcgga cctgccccag
     1261 gaccggctcg acggcctgac cgtcgacggc ggcgactggt ggttcaacct ccgaccgtcc
     1321 aacaccgagc ccctcctgcg gctcaacctc gaggcccccg acgcagaggc gtgcacccgg
     1381 cgcaccgagg aagtccgcgc cctgctcacc gcgtgaccag tccgtccaag gagaccacca
     1441 ccatgacgac cgaccagacc ctcgatcccc ggctgctgga gatcctggcc tgccccgagg
     1501 acaagggccc gctgctgtac ttcgccgacg agcagtcgct gtacaacccc cggctgcacc
     1561 gccgctacga cgtacgcgac ggcatcccgg tcatgctgat cgacgaggcc tcctcggtcg
     1621 acgacgccga gcacgaccgg ctgatggcca aggccggcgc cgacggcatc gaacccacgt
     1681 tctccgagtg agcgcaccgg tcgacggggc ggggacgcag ggggtcgggt cggtgctcga
     1741 ctcgctcggc atgttcgccg aggcctacgc gctgcccgag cagatggctg cggcccgggt
     1801 gtcggcagcg tccgcggacg gtctgcccga cgccgccggg gtcacttcgg tggtcatcct
     1861 cggcgtcggt ggcagcggca tcggaggcga catctgcgcg gcggtggcgg gcccggactg
     1921 cccggtgccg gtcgtggtgt ccaagcacta cgagtgtccg ggcttcgtcg gccccgacac
     1981 gctcgtgttc gcggtgtcgt tctcgggcaa caccgaagag gcgatcggag ctgcgaccgc
     2041 cgcacgcgac gccggtgcgc ggctggtcgt ggtcgccgcc ggcggcgaac tggcccggct
     2101 ggccggcgag tggggggttc ctctgctccc ggtcgacgac tcgatcccca tgccgcgggc
     2161 cggcatcggg gcggtgacgg tgccgttgct ggtcgccctg gagcggatgg ggctcaccga
     2221 cgggatgacc ggccagatcg acgccgcgat tgcccagctc gagcgacggc gggagcagct
     2281 ccgcgtcgcg tccggccctg cggagcgcct ggcggcgcgc atcgggggga cgttgccgct
     2341 ggtgtacggc ggcggagcgc tcggcgaggt ggccgcgtgg cgctggaaag gccagttcaa
     2401 cgagaacccc aaggtcccgg cgttctgcaa ccggatcccg gagctgaccc acaacgagct
     2461 ggccggatgg gcccagcacg gcgacgtcac ccgccaggtg ctgacgatgg tgcatctgcg
     2521 ccacgacttc gagcacccga acgtgtcgcg tcgcttcgac ttcgtggcct cggtctgtga
     2581 tgaagtggtg gccgacatcc acaccgtgcg ggccgaaggc gacggtcgtc tggcccagct
     2641 gatggacctg gtcctgcagg gggatctcgt cacgctgcac ctggcggcga gggcgggggt
     2701 cgacccgggc cccatcgcgg tgctcgacga tctgaaggaa tggctgcgca acgcctgacg
     2761 tcacggaccg ggcggtccgg gccggggggg tcagccggac gagggggagc cacgatgctc
     2821 ggagccgaca gcacgaccga cgacgtcctc gcgggtgtcg acctgaccgg acgcaccgcg
     2881 gtcatcaccg gctcgtccgg tgggctcggt ctcgagacgg cccgggccct ggcggctcgg
     2941 ggcgccgcgg tggtcctcgc ggcgcgtgac cgtgtcaagc tcgacgccgc ggtggcgcag
     3001 ctgcgcgacc aggtccccgg catcaccgtc gactcggtgc tcctcgacct ggccgacttg
     3061 acctcggtcc gggctgcagc ggccgaggtg gccgatcggc atccggtcat cgacctgctg
     3121 atcgacaacg ccggggtgat ggcctgtccg ctcggtcgca ccacggacgg cttcgagacc
     3181 cagttcggca ccaaccacct ggggcacttc ctgttcacga ccctgctcaa ggagcccctg
     3241 gccaccgccg tggcagagca cggatccgcc cgggtcgtcg tgctgtcctc gggtgggcac
     3301 cggctcagcc cgatccgctg ggacgacccc aactgggagc gctcggagga ttacttcaac
     3361 tggaccgcgt acgggcagtc caagaccgcc aacgcgctgt tcgctctgga gctcgaccgc
     3421 cggtggtcct ccgacggcat ccgggcgttc tcggtgcacc ccggcatgat cgccaccgac
     3481 ctgggccggc acatgcaggc cgaggacttc gagtacctgt cgtcggtgcg ccgcaccggc
     3541 gccgactccg ccgacggcgg gcgggcggtg ttcaagtcgg ttccgcaggg agcggcgacg
     3601 acggtgtggg cggccacgtc gcccgccctc gaggcgcacg gcggcgccta cctggagaac
     3661 tgctcggtgg ccgaaccggt cgacgacccc gacgccaccc acggagtgcg ctcctgggcc
     3721 cgggaccccg atcaggcggt gcggctctgg gagctgtccg aggatctggt cggcgcctga
     3781 cgccgtccgg gcgcggctcc ggacccacc
//
LOCUS       DAOQOD010000247         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11275_length_3799_cov_11.434295, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000247 DAOQOD010000000
VERSION     DAOQOD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11275_length_3799_cov_11.434295"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLZ72_15310"
     CDS             complement(<1..443)
                     /locus_tag="PLZ72_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37716.1"
                     /translation="MSDVGAETGPIPGARWRSAVAVLLVALTVLGATALTDDSWPFAP
                     FRMFAHPVNSTGRVAKVDFTALTASGRELRLDAEAFGLRRAEVEGQKDSSGRLTEAQM
                     AGLAAAWNRAHPSDPLVELRFRKFGRNLIDGHPVSDFSRVVQTWTA"
     gene            575..1618
                     /locus_tag="PLZ72_15315"
     CDS             575..1618
                     /locus_tag="PLZ72_15315"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNA37717.1"
                     /translation="MLVRSPFPIPFGWFQVAWSTDLAPGEIQPIEYFDRKMVLWRDGD
                     GAAHVSDAFCPHLGAHFGYGGHVNGNDIVCPFHGWRFDCDGRNTLIPYSERTNKKACV
                     KPYPVREVNGLIMAWYHPEEAEPTWEIPVVPEFNDPENFTAIETREYLIGAPWQDLAE
                     NGVDAAHFRYVHHTEEVPELESYETDGPLSKMRSIQKFPTPRGVVDGRIDADSFGPGF
                     SVIRFSGIVDTYLMGCNTPLTRNSCFMRFTFTVRKLGDDALSSTVGEAFVAEIDKQVQ
                     EDRPIWQNKAYITRPALADSDGPFTKFRKWASQFYAEGIDESQSVYYPVETKAYDPIQ
                     TASRKFGSDPMAD"
     gene            complement(1651..2457)
                     /locus_tag="PLZ72_15320"
     CDS             complement(1651..2457)
                     /locus_tag="PLZ72_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37718.1"
                     /translation="MPVTSSSAGRGLLIAGSTMMVLAVVGGFLVVSLLGSRLDLDRFT
                     RDVVIDGDRTPSVPGTLPFSVDAPLDGSSADTMAVGIAVEDRGIGTPECTLSTSSGDP
                     VALSRTSFDPALINGPDSWVVVASARLEPGDYRADCRWPGEPSSTPALVSFTVGRTLD
                     TSDVSALVAPMLGIVGVMAVAGLVFIVGLVLLIVGLVRRNRDRRPPSGRPGSGQPGSG
                     QPVWGPPAGYGQPPPGWGPPGAPPTQAPPVPPVPPVPPEMPNWPTPPGQG"
     gene            complement(2478..3650)
                     /locus_tag="PLZ72_15325"
     CDS             complement(2478..3650)
                     /locus_tag="PLZ72_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNA37719.1"
                     /translation="MGANRVAVIGVGQTKYASVRGDVSLPGLLREAAYRALADAHLTM
                     DDIDAVVVGKAPDFFEGIMMPEPYLAEALGAVGKPLFRVHTAGSVGGSTAIVAASHVE
                     AGVHERVLTIGWQQQSVSEAMWALSFPIPFQQQLVAGAGGYFAPFIREYMRRSGAPDD
                     IGILVALKDRLNALKNPYAHLHEPDITYDSIKESFMLWEPIRYAETCPSSDGAFAMVL
                     GGERAAEEARAATGVPPAWIHATAMRSEPSTASGRDQVRPQASLDCAADLYRQAGITD
                     PRAQIDVAEIYVPFSWYEPMWMEGLLFAEEGDGWKMTESGATALTGDLPVNCSGGVLS
                     TNPIGASGMIRFGEAAMQVRGQAGDHQVDGARVALGHAYGGAAQFYAMWIVSSDRP"
BASE COUNT          588 a         1363 c         1332 g          516 t
ORIGIN      
        1 gccgtccagg tctgcacgac ccgcgagaag tccgataccg gatggccgtc gatgaggttc
       61 cgaccgaact tgcggaaccg gagctcgacc agagggtccg aggggtgggc ccggttccag
      121 gcagcggcca gccccgccat ctgggcctcg gtcagcctgc ccgacgagtc cttctgaccc
      181 tcgacctcgg cccgtcgcag cccgaacgcc tcggcgtcga ggcggagctc gcggcccgac
      241 gcggtgagcg ctgtgaagtc gaccttggcg acccgaccgg tggagttcac cgggtgggcg
      301 aacatgcgga acggggcgaa gggccagctg tcgtcggtga gggcggtcgc tccgagcacg
      361 gtgagggcca ccagcagcac cgccaccgcg gaccgccaac gggctccggg gatcggtccc
      421 gtctcggcgc cgacatcgct cacgggcgag acactacggg ccccgccgcg ggcgaccgtg
      481 ggtctcgacc tcgcaagtcc gtacggactg tcacctgatc ggatccgtcc actagagtag
      541 aacgcgttct agtttctccc cgaggagcgt gcgcgtgttg gtccgatcgc cattccccat
      601 cccgttcggc tggttccagg tggcgtggtc caccgatctc gcccccggag agatccagcc
      661 catcgagtac ttcgaccgca agatggtcct ctggcgcgac ggcgacggtg ccgcacacgt
      721 cagcgacgcg ttctgccccc acctcggcgc ccacttcggc tacggcggcc acgtcaacgg
      781 caacgacatc gtgtgcccgt tccacggctg gcgcttcgac tgcgacggcc gcaacacgct
      841 catcccctac tcggagcgca ccaacaagaa ggcgtgcgtg aagccgtacc cggtgcgtga
      901 ggtcaacggc ctgatcatgg cctggtacca ccccgaggag gccgagccca cctgggagat
      961 cccggtggtg cccgagttca acgaccccga gaacttcacc gcgatcgaga cccgcgagta
     1021 cctgatcggt gcgccgtggc aggacctcgc cgagaacggc gtcgacgccg cccacttccg
     1081 ctacgtgcac cacaccgagg aggtgcccga gctcgagagc tacgagaccg acggacctct
     1141 ctcgaagatg cgctcgatcc agaagttccc gacgccccgc ggcgtggtcg acggtcgcat
     1201 cgacgccgac tcgttcggcc ccggcttcag cgtcatccgc ttctccggca tcgtcgacac
     1261 ctacctcatg ggctgcaaca cgccgctgac ccgcaacagc tgcttcatgc gcttcacgtt
     1321 caccgtgcgc aagctgggcg acgacgccct cagctccacc gtcggcgagg cgttcgtggc
     1381 cgagatcgac aagcaggtcc aggaggaccg gccgatctgg cagaacaagg cgtacatcac
     1441 ccgccccgcc ctggccgact ccgacggtcc gttcaccaag ttccgcaagt gggcgtcgca
     1501 gttctacgcc gagggcatcg acgagtcgca gtcggtgtac tacccggtcg agaccaaggc
     1561 ctacgacccg atccagaccg cgtcccggaa gttcggctcg gacccgatgg ccgactgagc
     1621 cccgaccgtc cgagccccgg ccggccgacc tcagccctga ccgggcgggg tcggccagtt
     1681 cggcatctcc ggcggcaccg gcggcaccgg cggcaccggt ggggcctggg tcggcggcgc
     1741 gccgggcgga ccccagcccg gcggaggttg cccgtacccc gccggcggac cccacaccgg
     1801 ttgacccgag cccggttgac ccgagcccgg tcgaccagac ggcggtcgcc ggtcgcggtt
     1861 gcggcgcacc agtccgacga tgagcaggac caggccgacg atgaacacca ggccggccac
     1921 ggccatgacc ccgacgatgc cgagcatcgg agcgaccagg gcgctgacgt cggaggtgtc
     1981 gagggtccgc ccgacggtga agctcaccag cgccggggtg gaggacggct cacccggcca
     2041 gcggcagtcg gcccggtagt ccccgggttc gaggcgtgcg ctggccacga cgacccacga
     2101 gtcgggtccg ttgatcaggg cggggtcgaa cgatgtccgg ctcagcgcga ccggatcgcc
     2161 cgacgaggtg gacagggtgc actcgggcgt gccgatcccc cggtcctcga ccgcgatccc
     2221 gaccgccatc gtgtcggcgg aggacccgtc gagcggggcg tcgaccgaga acggcagggt
     2281 tccgggcacc gacggggtcc ggtccccgtc gatcacgacg tcgcgggtga agcggtcgag
     2341 gtcgagccgg ctgccgagca gcgacacgac caggaacccg ccgacgacgg cgagcaccat
     2401 catggtcgac cccgcgatga gcaggcccct gcccgccgac gacgacgtga ccggcacgcc
     2461 cggaggcgtc tgggtgctca gggcctgtcc gagctgacga tccacatggc gtagaactgc
     2521 gcggcgccgc cgtaggcgtg gccgagggcg acccgggccc catcgacctg gtggtcgccg
     2581 gcctggcccc gcacctgcat ggccgcctca ccgaagcgga tcatgcccga ggccccgatc
     2641 gggttggtcg acagcacgcc gccggagcag ttgaccggca ggtcgccggt gagcgccgtg
     2701 gcccccgact cggtcatctt ccagccgtcg ccctcctcgg cgaacagcag gccctccatc
     2761 cacatcggct cgtaccacga gaacgggacg tagatctcgg cgacgtcgat ctgggcccgg
     2821 gggtcggtga ttccggcctg gcggtacagg tccgcggcgc agtcgaggct ggcctggggc
     2881 cgcacctggt cccggccgga ggcggtcgac ggctcggagc gcatggcggt ggcgtggatc
     2941 cacgccggcg ggactccggt ggcggcccgg gcttcctcgg ccgcgcgctc gccgccgagc
     3001 accatggcga aggcgccgtc ggaggacggg cacgtctcgg cgtagcggat cggctcccac
     3061 aacatgaacg actccttgat cgagtcgtag gtgatgtcgg gctcgtgcag gtgcgcgtac
     3121 gggttcttca gcgcgttgag ccggtccttg agggcgacca ggatgccgat gtcgtcgggt
     3181 gcgccggacc gccgcatgta ctcccggatg aacggggcga agtagccgcc ggccccggcg
     3241 accagctgct gctggaacgg gatcgggaac gacagcgccc acatggcctc ggacaccgac
     3301 tgctgctgcc agccgatggt cagcacccgt tcgtgcacgc cggcctcgac gtgcgacgcg
     3361 gcgacgatgg cggtcgaccc gccgaccgag ccggcggtgt ggacccggaa caggggcttg
     3421 cccaccgcgc cgagcgcttc ggccaggtac ggctcgggca tcatgatgcc ctcgaagaag
     3481 tccggcgcct tgccgaccac gacggcgtcg atgtcgtcca tggtgagatg cgcgtcggcg
     3541 agggcgcggt aggcggcctc gcgcagcagg ccgggcagcg acacgtcgcc acgcaccgag
     3601 gcgtacttgg tctggccgac gccgatcacg gcgacccggt tggcacccat caggactccc
     3661 cctcgagcac ggcgatcagg ttctgttgca gcagctggcc gccggtggcg tgggcgacgc
     3721 cgcggtcggc ggtaccggcg gagatccggt cggccacctc ggcgatgcgg atcagcccgg
     3781 cggccagcat cgggttggc
//
LOCUS       DAOQOD010000248         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11499_length_3757_cov_11.405457, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000248 DAOQOD010000000
VERSION     DAOQOD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11499_length_3757_cov_11.405457"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PLZ72_15330"
     CDS             <1..1759
                     /locus_tag="PLZ72_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37720.1"
                     /translation="PQRDVALAGMDRDGQVAVGNVSSTLLVADPLAPFGVRSDDDAVQ
                     ASVQRGWDAADVQVVEMSDLERAEDARPQSTVSQGDAQFSAALSRADRTFGRILATMD
                     LRRDLVMVVAPTAPAAGEQLTMFAMRGPGIDAGWARSPTTRRTGFVTLTDIAPTILDA
                     FDVEAPSEMNDTPITSVADDASLVDRADRMVRADERARFRDEVTGPITVAFIVGLVAL
                     LIAVMVAVGRDSSWRRPLRWCSLAVIATPSMMFLSGLLPYDPFSVATFGLTIAAGSAL
                     AAAVAAWVGRSDDVAAPVLLCGLSVVVLGVDILTGASLQLSTVFGYSPIVAGRFAGYG
                     NQAFSILTISSLVVVSGLWEIWGRRRPDSGDGGRLAVAVVVFLVVIVLDGFPLWGSDV
                     GGVLASIPAFAVCILVLKGIRIRFRLVALIAAVTVGVLVLFAALDLARPVDSRTHLGR
                     FAQKLLDGDGMLILQRKIDANASILTSTVWTLVIPVALLFVGYLTWRPNRMMRRLTSE
                     HPSFRAFGISGLTLGVLAWLVNDSGVAIPALMLTVALPYTAYLVLGLDGTDPPEPTSG
                     EVGGSVSAPPDGEPVDVTA"
     gene            1756..2604
                     /locus_tag="PLZ72_15335"
     CDS             1756..2604
                     /locus_tag="PLZ72_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37721.1"
                     /translation="MISTAIGLLIGGLVGAGCWWLTSDAFGVDPLARTNYRGAPVVTG
                     VGILIPVTVVLVVAAGRLVIAADDVIVSWDRLAAGTFVAAAGFCLLGLFDDVAGVGQS
                     GGFRGHVASLRHGRVSSGMLKLVGGAAVGILTVSVLQQDNGTVVGLLRDGATVALAAN
                     LGNLFDRAPGRATKFTATLFVVLAAVAATSWLFVPAVAVGAGVGLLAPDLREKAMLGD
                     AGSNVMGAMCGIAALVAFPAAPARWIVLAVLVGFNLLSEAVSFSAVIDAVAPLRWFDR
                     LGSQRR"
     gene            complement(2627..>3757)
                     /locus_tag="PLZ72_15340"
     CDS             complement(2627..>3757)
                     /locus_tag="PLZ72_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNA37722.1"
                     /translation="APRMEPLHGPDAVRADRSKAPARHRPRHRSTGLVAKTGLVAKIV
                     LVAKTGLVAMLALLVGACSNDDTPSATDRADRSSSTTSTSTPDASTVAEVLALDRPVV
                     LAHAGGDDIHPHDTPFGFDRSAAAGVDVLDMDVQLSSDGVLVVQHDDTVDRTTEAEGK
                     VADMTYEQLNALDAAYWFTEGCTCTDRPEAEYVYRGVRTGDRPAPEGSSPDDFAITSF
                     ETIAADHPDFVLNVEIKGTAPDALPAAAELARLVDELDLADRIVVTSFDDAVAEAFAS
                     AAPGATITPGLGATSAYILGGTLPPAGRTILQVPPEYEGLEVVTADRVATAHRDGLVL
                     WVWPNDAEWENADGYAKLLDMGVDGLNAADPVTALGVVRARR"
BASE COUNT          421 a         1301 c         1405 g          630 t
ORIGIN      
        1 gccccagcgc gacgtggcgc tcgccgggat ggaccgcgac ggacaggtcg cggtcggcaa
       61 cgtgtcgtcg accctgctgg tggccgaccc tctcgctccg ttcggagtcc gatccgacga
      121 cgacgcggtg caggcgtcgg tgcagcgcgg gtgggacgcc gcggacgtcc aggtcgtcga
      181 gatgtcggat ctggaacggg ccgaggacgc ccgtccccag agcaccgtgt cccagggcga
      241 tgcccagttc tccgccgcgc tcagccgggc cgaccggacc ttcggccgga tcctggcgac
      301 gatggacttg cgacgggacc tggtgatggt ggtcgccccc accgcacccg cggcgggcga
      361 gcagctgacg atgttcgcca tgcgcggtcc cggcatcgac gccgggtggg cccggtcgcc
      421 cacgacccgc cggacagggt tcgtcaccct caccgacatc gccccgacga tcctcgacgc
      481 cttcgacgtc gaggcgccga gcgagatgaa cgacaccccg atcacgtcgg tggccgacga
      541 cgcctcgctc gtcgatcgtg ccgaccgcat ggtccgcgcc gatgagcggg cccggttccg
      601 cgacgaggtc acgggtccga tcaccgtcgc gttcatcgtc ggtctggtgg ccctgctgat
      661 cgcggtgatg gtggcggtgg gccgtgacag ctcgtggcga cggccgctgc gctggtgctc
      721 gctggcggtg atcgccacgc cgtcgatgat gttcctgtcg ggcctgctgc cgtacgaccc
      781 gttctcggtc gccacgttcg gcctgacgat cgcggccggg tccgcgctgg ccgcggcggt
      841 cgcggcgtgg gtcggtcgct ccgacgacgt ggccgctccg gtcctgctgt gcgggctgtc
      901 ggtcgtggtg ctcggcgtcg acatcctcac cggcgcgagc ctgcagctca gcacggtgtt
      961 cggctactcg ccgatcgtcg ccgggcggtt cgccggctac gggaaccagg cgttctcgat
     1021 cctcaccatc tcgtcgttgg tggtcgtgtc ggggttgtgg gagatctggg ggcggcggcg
     1081 cccggactcc ggcgacggcg gtcggctggc ggtcgcggtg gtggtgttcc tggtggtgat
     1141 cgtgctcgac gggttcccgc tgtggggttc ggacgtggga ggcgtcctgg cgtcgatccc
     1201 cgcgttcgcg gtgtgcatcc tcgtgctgaa ggggatccgc atccggttcc gcctggtggc
     1261 gctgatcgct gcggtgaccg tcggggtgct ggtgctcttc gcggcgctgg acctggcccg
     1321 tccggtcgat tcgcggaccc acctgggacg gttcgcccag aagctgctcg acggcgacgg
     1381 gatgctgatc ctgcaacgca agatcgatgc caacgccagc atcctcacct cgacggtgtg
     1441 gaccctggtc atccccgtcg ccctgctctt cgtcggatac ctgacctggc gtcccaaccg
     1501 gatgatgcgg cgcctcacct ccgagcaccc gtcgttccgg gccttcggga tctcggggct
     1561 caccctcggt gtgctggcct ggctggtgaa cgactccggg gtggccatcc cggcgctgat
     1621 gctcaccgtc gcgctgccct acacggcgta cctcgtgctc ggcctcgacg ggaccgatcc
     1681 gccggaaccg acctccggag aggtcggggg ctccgtgtcc gcgccgcccg acggagagcc
     1741 cgtggacgtg accgcgtgat cagcaccgcg atcggactgc tgatcggagg gctggtcggc
     1801 gccgggtgct ggtggctcac ctccgacgcg ttcggcgtcg accccctggc ccgcaccaac
     1861 taccggggcg ctccggtggt caccggggtg gggatcctga tcccggtcac cgtcgtcctc
     1921 gtcgtcgccg ccgggcgcct ggtcatcgcc gctgacgacg tgatcgtgtc gtgggaccgc
     1981 ctggccgccg ggacgttcgt ggccgcggcc ggcttctgcc tgctcggcct gttcgacgac
     2041 gtcgccgggg tgggccagtc cggcgggttc cgcggccacg tcgcctcgct gcgccacggc
     2101 cgggtctcga gcgggatgct caagctggtc ggcggagccg cggtcgggat cctgaccgtc
     2161 tcggtgctgc aacaggacaa cggaaccgtg gtcgggctgc tgcgcgacgg cgccacggtc
     2221 gccctggccg cgaacctggg caacctgttc gatcgtgctc cgggtcgggc gaccaagttc
     2281 accgccacgc tgttcgtggt cctggccgcg gttgcggcca cgtcgtggtt gttcgtgccc
     2341 gcagtcgcgg tcggcgcagg tgtgggcttg ctggccccgg acctgcggga gaaggcgatg
     2401 ctgggcgacg ccggttcgaa cgtgatgggc gcgatgtgcg gcatcgccgc cctcgtcgcg
     2461 ttccccgccg cgccggctcg ctggatcgtg ctcgcagtgc tcgtcgggtt caacctgctc
     2521 agcgaggccg tgtcgttcag cgccgtgatc gacgcggtgg ctccgctgcg gtggttcgac
     2581 cgcctcggat cccagcggcg ctgagcgcgc tccgagcagc cgatgctcag cggcgggccc
     2641 gcaccacccc gagcgcggtg accgggtcgg ccgcgttcag cccgtcgacc cccatgtcga
     2701 gcagcttggc gtagccgtcg gcgttctccc actcggcgtc gttgggccac acccacagca
     2761 ccaggccgtc gcggtgggcg gtcgcgacgc gatccgcggt gacgacctcg aggccctcgt
     2821 actcgggcgg gacctgcagg atcgtccgac cggccggagg cagggtgccg cccaggatgt
     2881 aggcgctggt cgcgcccagg ccgggcgtga tggtggcacc gggggcggcg gaggcgaacg
     2941 cctcggccac cgcgtcgtcg aacgacgtca cgacgatgcg gtcggccaga tcgagttcgt
     3001 ccaccaggcg ggccagctcg gcggcggcgg gcagggcgtc gggggcggtg cccttgatct
     3061 cgacgttcag gacgaagtcg gggtggtcgg cggcgatcgt ctcgaagctg gtgatggcga
     3121 agtcgtcggg ggagctgccc tccggcgccg gccggtcgcc ggtgcgcacg ccgcggtaga
     3181 cgtactcggc ctcgggccgg tcggtgcagg tgcagccctc ggtgaaccag taggccgcgt
     3241 cgagcgcgtt caactgctcg taggtcatgt cggcgacctt gccctcggcc tcggtggttc
     3301 ggtcgacggt gtcgtcgtgc tgcacgacca gcacgccgtc ggagctcagc tgcacgtcca
     3361 tgtcgagcac gtcgacgcct gcggctgcgg agcggtcgaa gccgaacggc gtgtcgtgag
     3421 ggtggatgtc gtcgccgccg gcgtgggcga gcacgaccgg tcggtcgagg gcgagcacct
     3481 cggcgaccgt cgacgcgtcc ggggtcgacg tcgacgtcgt cgacgagctc cggtccgccc
     3541 ggtcggtcgc gctcggcgtg tcgtcgttgc tgcacgcgcc gacgagcaga gcgagcatcg
     3601 ccaccagccc ggtcttcgcc accagcacga tctttgccac cagcccggtc tttgccacca
     3661 gcccggtcga tcggtgtcgg ggccggtgcc gcgccggggc cttcgaacgg tcggcacgga
     3721 cggcgtcagg gccgtggagc ggttccatgc ggggagc
//
LOCUS       DAOQOD010000249         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11566_length_3744_cov_12.834644, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000249 DAOQOD010000000
VERSION     DAOQOD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11566_length_3744_cov_12.834644"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1290
                     /locus_tag="PLZ72_15345"
     CDS             106..1290
                     /locus_tag="PLZ72_15345"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNA37723.1"
                     /translation="MLQIRRHLEPTLVERLRSNAAVALIGPRQIGKTTMALDVADTWP
                     EGAVYLDLERPADRRRLDDADSYLRAQAPRLVVLDEVHRMPDIFEILRGVIDDNRRSG
                     VTSGQFLLLGSASLDLGRMSAESLAGRIAYLDLTGIAVDEAAAAGVGADDLWVRGGYP
                     LSLAAATDELSLQWRSDLVRSYLERDVPMFAPRIPTETLRRLWTMLAHHSGRLLNASD
                     LARALGVSGPTVDRYIDLLVDLGLVRRLPSWRANVAKRVTKAPKVLVRDSGLLHALLE
                     LVDLDAVLSHPAAGASYETFVMENLIDAAGPGFQAHHYRTASGVEVDLVLVRGGRPEV
                     AIEVKRSRAPTPGAGLRRAMDDLGLDTAYLVHPDDGREAYRSSGVTVTGVSELVARLR
                     EL"
     assembly_gap    1707..1806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1807..3273
                     /locus_tag="PLZ72_15350"
     CDS             <1807..3273
                     /locus_tag="PLZ72_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNA37724.1"
                     /translation="PVTGGLFALLLMAIGRFAWRIYLDRATKADSDQQEPVIVFGAGE
                     AGLQIVKSMHRGGPYRVAAVLDDNPDLANLRVKGVPVRGDRTDIAAVAERTGARTIVI
                     AIPSAPSALIREIVHLADGADLDTLVLPPVTELLDSGAVGVADIRPLTEADLLGRREL
                     SLDIDAVAHYITGRRVLVTGAGGSIGSELCRQLHRFAPESLIMLDRDESALQGVQLSI
                     EGRSLLDSRDLVVACIRDRERMAEVFAEHRPHVVFHAAALKHLSLLEMHPDEGWKTNV
                     VGTANVLDLAVEYGVTHFVNISTDKAAQPTSVLGETKRLAEQLTAEAATRADGTYISV
                     RFGNVLGSRGSVLPLFREQVAHGGPVTVTHPDVTRYFMTIPEACELVVQAGALGDDGE
                     VMVLDMGEQVRIVELAERLIAESGRDIEIVFTGLRPGEKLAEELFSPTEQPMASAHPL
                     ISRVAVPPVPPHLVAVAHTDDGIVDLSDASEIRPAQSA"
     gene            complement(3352..>3744)
                     /locus_tag="PLZ72_15355"
     CDS             complement(3352..>3744)
                     /locus_tag="PLZ72_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37725.1"
                     /translation="DIDGHRAETRAEFEQLRLTTKADIEHLRIATKADIDGLRAATKA
                     DFNGLRGDFNGLRAEFEHLRTETNSGFRELHAVIDARTDLLRSEIAATAATLHTTMAE
                     KSTSQLRWIIATMLASYAVIAAMAGLWR"
BASE COUNT          535 a         1241 c         1262 g          606 t
ORIGIN      
        1 cgagatccag gcgaacgcct ggatctcgca ctacagaacg ggatgaggag ggccggggtc
       61 ccgcgcggac caggtaaatc catcaccagc tgttagattt gcaaggtgct tcagatccgt
      121 cggcatctgg aaccgacgct ggtggaacgg ttgcgctcca acgcggccgt cgccctcatc
      181 ggtccccgtc agatcggcaa gaccaccatg gccctcgacg tcgcggacac gtggcccgag
      241 ggtgcggtct atctggacct ggagcggccg gccgaccgtc gccgactgga tgatgctgac
      301 tcctacctgc gggcccaggc cccccggttg gtcgtcctcg acgaggtgca ccggatgccg
      361 gacatcttcg agatcctccg tggcgtcatc gacgacaacc ggcgcagcgg cgtcacgtcg
      421 ggccagttcc tgttgctggg ttcggcgtct ctcgacctgg ggcgcatgag cgccgagagc
      481 ctcgccggtc gtatcgccta cctggacctg accgggatcg ccgtcgacga ggcggcagct
      541 gcgggagtgg gtgcagacga cctctgggtg cgtggcggct acccgctcag cctcgcggca
      601 gcaaccgatg agctgtccct tcagtggcga tcggacctgg tgcgctccta cctcgagcgt
      661 gacgtcccga tgttcgctcc tcggatcccg accgagacgc tcaggcgcct gtggacgatg
      721 ctcgcccacc acagcggccg gttgctcaac gccagcgatc ttgcccgggc gctgggtgtg
      781 agcgggccca ccgtcgatcg ctacatcgac ctgctcgtcg acctggggct ggtgcggcgg
      841 ctgccgtcgt ggcgggcgaa cgtcgccaag cgggtgacca aggcccccaa ggtcctggtg
      901 cgtgactccg gcctgctcca cgccttgttg gaactggtcg acctcgacgc cgtgctgtct
      961 cacccggcag caggggcgag ctacgagacc ttcgtcatgg agaacctgat cgacgccgcg
     1021 gggcccggat tccaggccca ccactaccgc accgccagcg gcgtcgaggt cgacctggtc
     1081 ctggtgcgtg gcggccgtcc ggaggtcgcg atcgaggtca agcggtcccg cgcgccgacg
     1141 cccggtgcgg gtctgcgccg ggccatggac gacctcggcc tggacaccgc gtacctggtg
     1201 caccccgacg acggtcgaga ggcctaccgg agctccggcg tcaccgtgac cggggtgagc
     1261 gagctcgtcg cccggctgcg cgagctctga cgacccagcg ccaccccacc ccctcccatc
     1321 gttctgtagt gcgagatcca ggcgaacgcc tggatttcgc actacagaac gatgggaggg
     1381 gcactgcaga acggaggagc gcagcggcga ggacaagcgc tgatcttgag tggggttcaa
     1441 gtcatggtcg ccgtcaccgg gcaggacgcg ggtgccgtgg accagggccc gatgacccat
     1501 gtccgatcgt ccattgccca gtgacgggga gggcgatcgg gcttgaaact gagagacgtt
     1561 cagtgttcaa tcgagtggcc gacaggcagg tccgtggagg tttggggtga gtctggggat
     1621 gcgtgacgtc atcgaagccg tgtcgcggcg ccgtttcgcg gcgcaggccg gcttcgacgg
     1681 cctcatgtgg atcccgtcgc tgtgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnccgg tcaccggcgg cctgttcgcc ctgctgctga tggcgatcgg acggttcgcg
     1861 tggcgcatct acctcgaccg ggccaccaag gccgactccg accagcagga gccggtcatc
     1921 gtcttcggcg ccggtgaagc gggtctgcag atcgtcaagt ccatgcaccg gggtggcccc
     1981 taccgggtgg ccgcggtgct cgacgacaac ccggatctgg ccaacctgcg ggtcaagggc
     2041 gtccccgttc gcggggatcg caccgacatc gccgcggtcg ccgaacgcac cggggcccga
     2101 accatcgtca tcgccatccc ctcggcgccg tcggcgctca tccgcgagat cgtgcatctg
     2161 gccgacggcg ctgatctcga caccctcgtc ctgccgccgg tcaccgagtt gctcgactcg
     2221 ggcgcggtcg gcgtcgccga catccgtccc ctcaccgaag ccgatctgct cggccgccgc
     2281 gagctcagct tggacatcga tgccgtggcg cactacatca ccggccgccg ggtgctggtc
     2341 accggagccg gcgggtcgat cggctccgag ctgtgccggc agctgcaccg cttcgccccc
     2401 gagtcgctca tcatgttgga ccgcgacgag tccgccctcc agggggtgca gctgtcgatc
     2461 gagggtcgca gcctgctgga cagccgcgac ctggtcgtcg cgtgcatccg ggaccgtgag
     2521 cgcatggccg aggtgttcgc cgagcaccgc ccgcacgtgg tgttccacgc ggctgcgctc
     2581 aagcacctgt ccctgctgga gatgcatccc gacgagggct ggaagaccaa cgtggtcggc
     2641 accgccaacg tgttggacct ggcggtcgag tacggcgtga cgcacttcgt gaacatctcg
     2701 accgacaagg ccgcccagcc cacctcggtg ctgggcgaga ccaagcgtct ggccgagcag
     2761 ctcaccgccg aggccgccac ccgcgccgat ggcacctaca tctcggtgcg cttcggcaac
     2821 gtgttgggca gccggggttc ggtgctgccg ctgttccggg agcaggtcgc acacggcggt
     2881 ccggtcaccg tcacgcaccc cgacgtcacc cgctacttca tgaccatccc cgaagcgtgc
     2941 gagctggtcg tgcaggccgg cgctctgggc gacgacggtg aggtcatggt gctcgacatg
     3001 ggcgagcagg tccgcatcgt cgagttggcc gaacgcctca tcgccgagtc gggtcgagac
     3061 atcgagatcg tcttcaccgg gctgcggccg ggagagaagc tggccgagga gctgttcagc
     3121 cccaccgagc agcccatggc ctcggcgcac ccgctgatct cgcgggtggc ggtgccgccg
     3181 gtgcctccgc acctggtggc cgttgcacac accgacgacg ggatcgtcga cctgagcgac
     3241 gccagcgaga tccgccccgc ccagtccgcc tgagaggctc cggacgccac cctcgcgccg
     3301 acgcggggaa gaccctcccg ctccgcgagc cttccccgat ccgggcggag atcagcgcca
     3361 gaggccggcc attgcggcga tgacggcgta cgaggcgagc atcgtggcga tgatccaccg
     3421 gagttggctg gtggacttct ccgccatcgt ggtgtggaga gtggcggccg tcgcggcgat
     3481 ctcggaccgg agcaggtcgg tgcgtgcgtc gatcaccgcg tggagttcac ggaaccctga
     3541 gttcgtctcc gtgcggaggt gttcgaactc agctctgagc ccgttgaaat cgcccctgag
     3601 cccgttgaaa tcggctttgg tggctgctct gagtccgtcg atgtctgcct tggtggcgat
     3661 gcggaggtgt tcgatgtcgg ccttcgtggt gaggcgcagc tgttcgaact cggctctggt
     3721 ctcggcgcgg tggccgtcga tgtc
//
LOCUS       DAOQOD010000250         3733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11635_length_3733_cov_12.402393, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000250 DAOQOD010000000
VERSION     DAOQOD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11635_length_3733_cov_12.402393"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLZ72_15360"
     CDS             complement(<1..1735)
                     /locus_tag="PLZ72_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNA37726.1"
                     /translation="MISVSGLSKAHGGRTLFSDVSFRLVPGRRVALVGGNGVGKTTIL
                     EIVVGLQRSDTGEVHVAKGSRIGYLPQELTEQIDGTVIEEVMRGAAHITDLEDQLARL
                     AADVAATGPGGADEDDDAHQHALAAYGEVQHRFETLGGYGVEAEARRVLDGLGFTGTD
                     ADRPVQDLSGGWRMRVALARLMLSAPDLLVLDEPTNHLDVESVAWLEQYLQSWPGAIL
                     FVSHDRDFLDAVAERVIEVAQGRATEYVGGFAEFVVQREERHQAALAAAEQQQRQIAH
                     VERFVERFRYKATKARQVQSRLKMLEKLDRLEAPTVEQVQARFAFPPPRRSARVVAEI
                     EDATVGFDGVPVLSHVDLVLERGQKLALIGPNGAGKTTLLRLLLGRLDPMAGHAQLGA
                     NVDVAEFAQHQVDALDFDRTVLEELRRAVGDEHGRNLRSVLAAFGFKGDAVDRTVADL
                     SGGERTRLALARIMVEPVNLLVLDEPTNHLDLPSCDVLEDALVAYPGAVLLVTHDRYL
                     IRSVADALLEVRDGTVVYHPSVDEEVLAPRGATATAGRVAPAVASESGGPAGRRPNKA
                     EQRRASAGQRRA"
     gene            complement(1732..3390)
                     /locus_tag="PLZ72_15365"
     CDS             complement(1732..3390)
                     /locus_tag="PLZ72_15365"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNA37727.1"
                     /translation="MTDGGPVAHTYNLADQWEAVADRVAEREAVVAGDRRLTYAQLEE
                     RANRLANHLSSAGIGPGDFVGCYLTNGTEYLETMLACFKLRAVPINVNYRYVADELRY
                     LFTDAGLVAVVCEQRFAPRVAEIAADVPTLRHTLVVADPDADADAGAVDLASVPDGHD
                     YEAALAAASADRPVVADRGDDDIYVIYTGGTTGMPKGVVWRMGDAFFGCIGGGDPLRM
                     SGPVSSPEETVERIIDFDFNFYALAPMMHAAAQWVSMMWLLCGAKVVLHQGRFDPSVV
                     WRTVEAEKISFLTIVGDAMARPLLDDWDANGPYEVGSLYSFSNGGAPISPAARSRVQA
                     ILPNAMFTDGFGSSETGIQGSQRLQPGEDPGNVIRYDNVASGTKVVDLDGHEVEPGSG
                     VVGRIAHTGWIPLRYHNAPEKTAEAFFEVDGRRYVVSGDMATVEADGSVILLGRGSVS
                     INTGGEKVYPEEVEGVLKGHPAVYDTVVVGVPHERWGEQVVAVVQPVEGEQPTLDDIE
                     PFARTQLAGYKVPRRIVLVDEVVRSPAGKADYRWAKAVAADSTS"
     gene            complement(3416..>3733)
                     /locus_tag="PLZ72_15370"
     CDS             complement(3416..>3733)
                     /locus_tag="PLZ72_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37728.1"
                     /translation="RDLFVEGDRIVGVVMWSEVFARCEHPSVGVRWSDPTAVTVDGRL
                     VPIPRVSVNYQSHADGGCANTDSRPDATGVAQWTAVTRTVAQGTVLAVPGSGAAAPVP
                     SGP"
BASE COUNT          511 a         1309 c         1371 g          542 t
ORIGIN      
        1 gggcccgccg ctgccccgcg ctggcccgcc gctgctcggc cttgttcggt cggcgaccgg
       61 ccggcccgcc ggactcggat gccaccgcgg gagcgacgcg gcccgcggtc gcggtcgcgc
      121 cccggggcgc caggacctcc tcgtcgaccg acgggtggta cacgacggtc ccgtcgcgca
      181 cctcgagcag ggcgtcggcc accgagcgga tcaggtagcg gtcgtgggtg acgagcagca
      241 ccgccccggg gtaggcgacg agggcgtcct cgagcacgtc gcagctcggc aggtcgaggt
      301 ggttggtcgg ctcgtcgagg accagcaggt tgaccggttc gaccatgatc cgggccaggg
      361 cgaggcgggt ccgctcgccg ccggacagat ccgcgacggt ccgatcgacg gcgtcgccct
      421 tgaagccgaa cgcggcgagg acgctgcgca ggttgcggcc gtgctcgtca ccgacggcac
      481 ggcgcagctc ctcgagcacg gtgcggtcga agtcgagggc gtcgacctgg tgctgggcga
      541 actcggccac gtccacgttg gctccgagct gggcgtgacc ggccatcggg tccaggcgac
      601 cgagcaggag gcgcagcaag gtggtcttgc cggctccgtt gggtccgatc agggcgagct
      661 tctgtccccg ttcgagcacc aggtcgacgt ggctgagcac cgggacgccg tcgaagccga
      721 ccgtggcgtc ctcgatctcg gccaccaccc gggccgagcg ccgcggaggc ggaaaggcga
      781 aacgggcctg gacctgctcg acggtcggag cctcgaggcg gtcgagcttc tcgagcatct
      841 tgagccgcga ctggacctgg cgggccttgg tcgccttgta gcggaaccgc tcgacgaagc
      901 gttcgacgtg cgcgatctgg cgctgctgct gctcagccgc ggcgagggcg gcctggtggc
      961 gctcctcgcg ctgcacgacg aactcggcga agccgccgac gtactcggtg gcccgaccct
     1021 gcgcgacctc gatgacccgt tcggccacgg cgtcgaggaa gtcgcggtcg tggctgacga
     1081 acaggatcgc tcccggccag ctctgcaggt actgctcgag ccacgccacc gactcgacgt
     1141 cgaggtgatt ggtcggctcg tcgagcacca gaaggtcggg ggcgctcagc atcagccggg
     1201 ccagggcgac gcgcatccgc cacccacccg agaggtcctg caccggtcga tcagcgtcgg
     1261 tcccggtgaa cccgaggccg tcgagcaccc gtcgggcctc ggcctcgacg ccgtagccgc
     1321 cgagggtctc gaagcggtgc tgcacctcgc cgtaggcggc cagtgcgtgc tggtgggcgt
     1381 cgtcgtcctc gtccgctccg cccggcccgg tcgctgcgac gtccgcggcc aggcgggcga
     1441 gctggtcctc gaggtcggtg atgtgcgccg cgccgcgcat cacctcctcg atgacggtgc
     1501 cgtcgatctg ctcggtgagc tcctggggca ggtagccgat ccggctgccc ttggcgacgt
     1561 gcacctcacc ggtgtcggag cgctgcaggc cgacgacgat ctccaggatc gtggtcttgc
     1621 ccacgccgtt gccgccgacg agcgcgaccc gccgccccgg caccaaccgg aacgagacgt
     1681 cggagaagag ggtgcggccg ccgtgggcct tggagagtcc ggagacggag atcaagaggt
     1741 cgagtctgcc gcgaccgcct tggcccagcg gtaatcggcc ttgccggccg gggaccggac
     1801 cacctcgtcg acgagcacga tccgccgggg caccttgtag ccggcgagct gggtccgggc
     1861 gaacggctcg atgtcgtcga gggtgggctg ctcgccctcg accggctgca cgaccgccac
     1921 gacctgctcc ccccagcgct cgtggggcac gccgaccacg acggtgtcgt acaccgccgg
     1981 gtggcccttg aggacgcctt cgacctcttc ggggtagacc ttctcgccgc cggtgttgat
     2041 gctgaccgac ccgcgtccga gcaggatgac cgacccgtcg gcctcgacgg tcgccatgtc
     2101 tcccgacacg acgtagcgac ggccgtcgac ctcgaagaac gcctcggcgg tcttctcggg
     2161 ggcgttgtgg tagcgcagcg ggatccagcc ggtgtgggcg atgcggccga cgacgccgga
     2221 acccggttcg acctcgtggc cgtcgaggtc gaccaccttg gtgcccgagg cgacgttgtc
     2281 gtagcggatg acgttgcccg ggtcctcgcc gggctgaagc cgctggctgc cctggatgcc
     2341 ggtctcggag gacccgaagc cgtcggtgaa catggcgttg ggcaggatcg cctgcacccg
     2401 cgagcgggcg gccgggctga tcggggcgcc gccgttggag aacgagtaca gcgatccgac
     2461 ctcgtagggt ccattggcgt cccagtcgtc gagcaggggg cgggccatgg cgtcgccgac
     2521 gatggtgagg aagctgatct tctcggcctc gacggtgcgc cacacgacgc tcgggtcgaa
     2581 gcggccctgg tgaagcacca ccttggcccc gcacaggagc cacatcatcg acacccactg
     2641 cgcggcggcg tgcatcatcg gggccagcgc gtagaagttg aagtcgaagt cgatgatgcg
     2701 ttcgacggtc tcctcgggcg atgagaccgg cccgctcatg cgcagggggt cgcccccgcc
     2761 gatgcagccg aagaaggcgt cgcccatgcg ccacaccacg cccttgggca tgccggtggt
     2821 gccgccggtg tagatgacgt agatgtcgtc gtcaccccgg tccgccacga ccgggcggtc
     2881 cgccgacgcg gcggcgagcg ccgcctcgta gtcgtggccg tcgggaacgg aggccaggtc
     2941 gaccgccccg gcatctgcgt cggcgtccgg atcggcgacc accagcgtgt ggcgcagggt
     3001 cggcacgtcg gccgcgatct cggccacccg gggggcgaag cgctgctcgc acacgaccgc
     3061 caccagtccg gcgtcggtga acaggtagcg gagctcgtcg gcgacgtagc ggtagttgac
     3121 gttgatcggc accgcccgca gcttgaagca ggcgagcatg gtctcgaggt actccgtgcc
     3181 gttggtcagg tagcagccga cgaagtcgcc gggcccgatc cccgccgagc tcaggtggtt
     3241 ggccagtcgg ttggcccgtt cctcgagctg cgcgtaggtc agccggcggt cccccgcgac
     3301 gacggcttcg cgctcggcga cccggtcggc gacggcctcc cactggtcgg ccaggttgta
     3361 ggtgtgcgcc accggcccac cgtcggtcat gtcggctccc ctcggttcgt ccccgtcacg
     3421 gtccggacgg taccggagcg gccgctccgc tgccggggac ggccagcacg gttccctggg
     3481 ccacggtgcg ggtgaccgcg gtccactggg ccaccccggt cgcatcgggc cgcgagtcgg
     3541 tgttggcgca gccgccgtcg gcgtggctct ggtagttgac cgacacccgg gggatcggga
     3601 cgagacgtcc gtccacggtc accgcggtcg gatcggacca gcgcaccccg accgacgggt
     3661 gctcgcagcg ggcgaacacc tcggaccaca tgacgacgcc cacgatgcgg tccccctcga
     3721 cgaacaggtc gcg
//
LOCUS       DAOQOD010000251         3704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11799_length_3704_cov_10.392162, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000251 DAOQOD010000000
VERSION     DAOQOD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11799_length_3704_cov_10.392162"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1237
                     /locus_tag="PLZ72_15375"
     CDS             41..1237
                     /locus_tag="PLZ72_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37729.1"
                     /translation="MDLTWSDAEEAFRQECRTWLTEHAPNPALSSGDTAAGFAAHVEW
                     EKELHADRWAVVSWPEAYGGRDASLWEWLIFEEEYYRAGAPQRVTQNGIFLLAPTIFE
                     FGTPAQQDHYLPRMSAAEDLWCQGWSEPNAGSDLAGITSRAVRDDAAGGWLLSGQKTW
                     TTRGAFCTHLFGLFRTDGEAERHRGLTYFLVPLDADGVTVRGFGRLDGDEGFAEVFLD
                     DVFVPDNPDEGISPADAAATMSRGGTLGGIDQGWGVAMATTTSERGLTLRSPGRFRAA
                     AARLLDLAREVDGSGRLTERLGDRVVDAWIDAEAYALYTLGDVTGIVEGRSPGARSSF
                     NKVFWSELDIRLHRAALDLLGPDAELDGPWMKGYEFALSGPIYAGTNEIQRNIIAERV
                     LGLPRK"
     gene            1251..2288
                     /locus_tag="PLZ72_15380"
     CDS             1251..2288
                     /locus_tag="PLZ72_15380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37730.1"
                     /translation="MRFAPTEDQIDFRDAVRGLLADTCPPDAVRAAWREQVGEVPGGG
                     DGRVRAAWDALAEMGVLGVMVPEGSGGLGLTDEDIVPLLVECGRAALPDPVSSTAYVA
                     AGLLRENPGRSTEALLARIAEGSATVLVGFPGSPYLTAAETADVFLMCDRGEVHELTR
                     SEVTLEPVTSVDGARALCRVEWEPSDRSVIVGGFDGSQAVTRAFDRAALGAAAQLVGL
                     GERMLDLTVGYAAERKQFGVAIGTFQAVKHHLADAASAVAFAQPLVLRAAYSLAQGDP
                     DAPVHVSMAKARASAAALVVAEAALQCHGAIGYTVEYDLHFFMKRAWALASRYGDADF
                     HRDRVRAVLLG"
     gene            complement(2357..>3704)
                     /locus_tag="PLZ72_15385"
     CDS             complement(2357..>3704)
                     /locus_tag="PLZ72_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNA37731.1"
                     /translation="QDRHRRVAELLGITGGVSVPAVAAAALRQANVDLADARAAAERA
                     TVTLARIEELRAELAGVRERADRLRLEAAGAAARIDALREEADALAEQIGRAVGGPHR
                     SGATDRDQDGLLSELATTRRAVTEVIEAAAAAVTALRRLDAASDAARVAADRVDQQIA
                     ASVFGSVADARAALIADDELLQLDTRIRAHHDRLAGVEAGLAADDLADLPDLRPDVAA
                     AEHAWETARLAVDLATTQEERSRSTADAISRWSDEHRALELHTASARRDEAALERLAE
                     TLSGRAGSKVSLQRWVLAAFLDDICQLANGRLATMTGGRYSLSVHRGDSPGRRAAGLD
                     LRVLDSHTGTERDVSTLSGGETFQASLALALAVADAVEQHAGGIRMEALFIDEGFGSL
                     DPDALELAMDELDALRAGGRMVGVISHVAGLQERIRTGIRVEPSEHGSTARVGRIF"
BASE COUNT          469 a         1346 c         1379 g          510 t
ORIGIN      
        1 cggatctgac gcctcgtcag gtgtcgggca gactggcgtg atggacctca cgtggagcga
       61 cgccgaggag gccttccgcc aggagtgccg gacctggctg accgagcacg cccccaaccc
      121 ggctctgtcc tccggcgaca ccgcggccgg cttcgccgcg cacgtcgagt gggagaagga
      181 gctgcacgcg gatcgttggg cggtcgtgtc gtggcccgag gcctacggcg gtcgtgacgc
      241 gtcgctgtgg gagtggctga tcttcgagga ggagtactac cgggccggtg ccccccagcg
      301 ggtcacccag aacggcatct tcctgctcgc cccgaccatc ttcgagttcg ggacgcccgc
      361 ccagcaggac cactacctgc cgcggatgtc ggcggctgag gacctgtggt gccagggctg
      421 gtccgaaccg aacgccggtt ccgacctggc cgggatcacg tcccgggcgg tgcgcgacga
      481 cgcggcgggg ggatggctcc tgtcaggcca gaagacctgg acgacccggg gcgccttctg
      541 cacgcacctg ttcggtctgt tccgcaccga cggcgaggcc gaacgccacc gggggctcac
      601 gtacttcctg gtcccgctcg acgcggacgg cgtcaccgtg cggggcttcg gccgactcga
      661 cggcgacgag gggttcgccg aggtgttcct cgacgacgtc ttcgtccccg acaacccgga
      721 cgagggcatc tcccccgccg acgcggccgc caccatgtcg cgagggggaa ccctgggtgg
      781 catcgaccag ggctgggggg tggccatggc gaccaccacc tcggagcggg ggctcaccct
      841 gcgctcaccc ggtcggttcc gcgccgccgc ggcccggctc ctggacctgg cccgcgaggt
      901 cgacggttcg gggcggctga ccgagcgcct cggcgaccgg gtcgtcgacg cctggatcga
      961 cgccgaggcc tacgccctgt acaccctggg cgacgtcacc ggcatcgtcg agggccgttc
     1021 cccgggggcg cgctccagct tcaacaaggt gttctggtcc gagctcgaca tacgactgca
     1081 ccgggcggcg ctcgacttgc tcggcccgga cgccgagctc gacggcccct ggatgaaggg
     1141 ctacgagttc gccctgtcgg ggccgatcta cgccggcacc aacgagatcc agcgcaacat
     1201 catcgccgag cgcgtgctcg gcctgccgcg gaagtagcgg ggagcagacc atgcggttcg
     1261 caccgaccga ggatcagatc gacttccgcg acgccgtgcg cggcctcctg gccgacacgt
     1321 gcccgcccga cgcggtgcgc gccgcgtggc gggagcaggt gggtgaggtg cccggcggcg
     1381 gagacggccg cgtccgggcg gcgtgggacg ccctcgccga gatgggcgtg ctgggagtca
     1441 tggtgcccga gggctccggc ggcctcggac tcaccgacga ggacatcgtc ccgctgctcg
     1501 tcgagtgcgg acgggccgcg ctgcccgacc cggtgtcgtc gaccgcgtac gtcgccgccg
     1561 gcctgctgcg ggagaacccc gggcgcagca ccgaggcgct gctcgcccgc atcgcggagg
     1621 gatcggccac ggtgctggtc ggcttccccg gctcgccgta cctgaccgcg gcggagacgg
     1681 cggacgtctt cctgatgtgc gaccggggcg aggtccacga gctcacccgg tccgaggtga
     1741 ccctcgaacc ggtgacctcg gtcgacgggg cccgcgccct gtgccgcgtc gaatgggagc
     1801 cgtcggaccg ctcggtgatc gtcggcgggt tcgacgggtc ccaggccgtg acccgcgcct
     1861 tcgaccgcgc cgctctcggc gcggccgctc aactggtggg actcggcgaa cggatgctgg
     1921 acctgaccgt cggctacgcg gcggagcgca agcagttcgg ggtggcgatc gggacgttcc
     1981 aggccgtcaa gcaccacctg gccgacgccg cgtcggccgt cgccttcgcc cagccgttgg
     2041 tgctgcgcgc cgcgtactcg ctggcccagg gggacccgga cgcgccggtt cacgtctcga
     2101 tggccaaggc gcgcgcgtcg gccgccgcgc tcgtcgtggc cgaggcggcc ctgcagtgcc
     2161 acggggccat cggctacacc gtggagtacg acctgcactt cttcatgaag cgggcatggg
     2221 ctctggcgtc gcgctacggc gacgcggact tccaccgcga ccgcgtgcgc gctgtgctgt
     2281 tgggctgacg cgtgcgggtc ggctgacgcg gtcgtcccga cggcagcgcg gcgcagaact
     2341 gcgggagacg tcgaggtcag aagatccggc cgacgcgggc cgtggacccg tgctccgacg
     2401 gctcgacgcg gatcccggtg cggatgcgct cctgcagccc ggcgacgtgg ctgatcacgc
     2461 cgaccatgcg gccaccggca cggagcgcgt cgagctcgtc catggccagc tccagcgcat
     2521 cggggtcgag cgacccgaat ccctcgtcga tgaagagcgc ctccatgcgg atcccgccgg
     2581 cgtgctgctc gaccgcgtcg gcgaccgcga gcgcgagcgc gagggaggcc tggaaggtct
     2641 ctccgcccga cagggtggac acgtcgcgtt cggtgccggt gtgcgagtcg aggacccgca
     2701 ggtcgagccc ggccgctcgg cgtccggggc tgtcgccgcg gtgcaccgac aacgagtagc
     2761 gccccccggt catcgtggcc agccggccgt tcgcgagctg gcagatgtcg tcgaggaacg
     2821 ccgccagcac ccagcgctgg agcgacacct tggagccggc ccgaccgctg agcgtctcgg
     2881 ccagccgttc gagcgccgct tcgtcgcggc gagccgacgc ggtgtgcagc tccagcgccc
     2941 ggtgctcgtc ggaccagcgc gagatggcgt ccgcggtgct gcgggagcgc tcctcctgcg
     3001 tcgtcgcgag atccacggcg agtcgggccg tctcccacgc gtgctcagcg gcggccacgt
     3061 cgggacggag gtcgggaagg tccgccaggt cgtcggcggc caacccggcc tccactccgg
     3121 cgagtcggtc gtgatgggca cggatccggg tgtcgagctg cagcagctcg tcgtcggcga
     3181 tcagagcggc tcgggcgtcg gcgacggagc cgaacaccga cgccgcgatc tgctggtcga
     3241 cccggtcggc ggcgacgcgg gcggcgtcgg acgccgcgtc gagccgacgg agggcggtga
     3301 cagcggccgc ggctgcctcg atcacctcgg tcaccgcccg acgagtcgtc gccagctcgg
     3361 agagcaaccc gtcctggtcc cggtcggtcg cgccggagcg gtgaggcccg ccgacggctc
     3421 ggccgatctg ctcggcgagg gcgtcggcct cttcgcggag ggcatcgatc cgggccgccg
     3481 ctccagccgc ctcgagtcgg agccggtccg cccgttcacg cacccctgcc agctcggccc
     3541 gcagctcctc gatccgggcg agcgtgaccg tcgccctctc cgctgctgcc ctggcgtcgg
     3601 ccagatccac gttcgcctgc cggagcgccg ccgccgccac ggcggggacc gagacgccgc
     3661 cggtgatccc gagcagctcg gccacccggc ggtgccgatc ctgg
//
LOCUS       DAOQOD010000252         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11903_length_3684_cov_11.014329, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000252 DAOQOD010000000
VERSION     DAOQOD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11903_length_3684_cov_11.014329"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLZ72_15390"
     CDS             complement(<1..725)
                     /locus_tag="PLZ72_15390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNA37732.1"
                     /translation="MADLKVEVEPREVGLDADRLRRIDSHFARYVDDGRLPGWLAVVS
                     RHGRVAHIAHSGHRDVEAGAPITSDTLYRIYSMTKPITSVAAMMLYEEGAFELKDKVK
                     RYLPAFADTRVYKGGGAGSPQLEPQIDGMRIWHLFTHTSGLSYGFHHAHPVDALYRKH
                     GFEWGSPRGLDTAGTIDAVARLPLVFQPGTSFNYSMSTDVLGRLVEVWSGMGFDDFLR
                     TRIFEPLGMDETTFWVDGDDADRL"
     gene            complement(728..1072)
                     /locus_tag="PLZ72_15395"
     CDS             complement(728..1072)
                     /locus_tag="PLZ72_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37733.1"
                     /translation="MSDQGTPEDIDAAERSEAEEIRSRIADLPNALGLAARLNSGVLE
                     AGAALDMRTTHLVRVAAMAATGMPVMGWEVNLELMEDEVGVDDIEAVLAVIAPIIGTS
                     RYLQAVTTLVTD"
     gene            1279..2658
                     /locus_tag="PLZ72_15400"
     CDS             1279..2658
                     /locus_tag="PLZ72_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNA37734.1"
                     /translation="MTDTGLLDALAEVLAPDRVLGPEVASEDYSHDECLTVASRRPAA
                     VVLVESTAEVSAVVKLAAERKVPITARGSGTGLSGAAIPAEGAIVVSFERMNRVLELD
                     PDNHVAVVQPGLRLNELDEVTAAAGLVYPVYPGEYSASLGGNVATNAGGMRAVKYGVT
                     RHQVLGLELVLADGQIIRTGGKFVKATTGYDLTQLVIGSEGTLALVTEATLRLYPRPE
                     HQATVLAPFTTLEQVTAAVPRLIDSGVGPLILEYIDLLTMSATASFTGLELGIPQEIK
                     DSALAYLVVMLEDSDPTRLDDDIDSVAQQLHELGAIDAYVLPPAAAGQLIDAREKAFW
                     MAKANGADDVVDIVVPRAQIPAFMEKVAALAGEYEAWVAGCGHAGDGNVHLGIFCGDP
                     DRRSRLLHALFAAGVALGGAISGEHGIGEAKQAYFLELEDPAKLALMRRIKAAFDPDG
                     ILNPGTLLG"
     gene            2711..>3684
                     /locus_tag="PLZ72_15405"
     CDS             2711..>3684
                     /locus_tag="PLZ72_15405"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNA37735.1"
                     /translation="MNGAQAMIRTLVDCGVDVCFMNPGTSEMHFVHALDSVPEMRGVL
                     ALFEGVATGAADGYARMADRPAAVLLHLGPGLGNGLANLHNARRAKVPVVNVVGDHAT
                     YHAKYDAPLQSDIISIARGVSSWVRTVASPETIARDTADAVAASYGAPGQVATLIVPA
                     DTCWLDSPGPVGPSPVSGAGTVPSDRIDDIAAVLRGGEPAGLLVGGAAMRTGALHDAA
                     RVGMATGAKVLSETFPTRAERGAGTPAVERLAYLAEFAQMQLQGLRHLVLVDAAHPAS
                     FFAYPDKASDLVAEGCQVHVLSAPDEDAAGALAALAAALDAPSDPPVIP"
BASE COUNT          516 a         1342 c         1291 g          535 t
ORIGIN      
        1 aggcggtcgg cgtcgtcgcc gtcgacccag aaggtcgtct cgtccatgcc caggggctcg
       61 aagatccggg tgcggaggaa gtcgtcgaag cccattcccg accacacctc gaccagtcgg
      121 ccgagcacgt cggtcgacat cgagtagttg aacgacgtgc ccggttggaa caccaggggg
      181 agacgggcga ccgcgtcgat cgttccggcg gtgtccaggc cccggggcga accccactcg
      241 aagccgtgct tgcggtacag ggcgtcgacc gggtgggcgt ggtggaaccc gtagctcagc
      301 ccggaggtgt gggtgaacag gtgccagatc cgcatgccgt cgatctgtgg ttccagctgt
      361 ggggagcccg ccccgccgcc cttgtagacc cgggtgtcgg cgaacgcggg caggtaccgc
      421 ttgaccttgt ccttcagctc gaacgcgccc tcctcgtaca gcatcatcgc cgcgaccgac
      481 gtgatcggct tggtcatcga gtagatgcgg tagagggtgt cggaggtgat cggggctccg
      541 gcttcgacgt cgcggtggcc gctgtgggcg atgtgggcga cgcggccgtg ccggctgacg
      601 acggcgagcc agccgggcag ccgtccgtcg tcgacgtagc gggcgaagtg gctgtcgatg
      661 cggcgcaggc ggtcggcgtc gaggccgacc tcccgtggct cgacctcgac cttgagatcg
      721 gccacggtca gtccgtgacc agggtcgtga cggcctggag gtagcgggag gtcccgatga
      781 tcggggcgat gacggcgagg accgcctcga tgtcgtcgac gccgacctcg tcctccatca
      841 gctcgaggtt gacctcccag cccatcaccg gcatccccgt ggccgccatg gccgccacgc
      901 ggaccaggtg ggtcgtgcgc atgtcgagcg cggctccggc ttcgagcacg cccgagttga
      961 gccgtgcggc caggccgagg gcgttgggca gatccgcgat ccgacttcgg atctcctccg
     1021 cctcggaccg ctcggcggcg tcgatgtcct ctggggtccc ctgatcgctc accgcgacct
     1081 cctgcgtcgt cgctcggacc ccggcggggc cgtgtcatcg gcacgctacc ggcgatcgcg
     1141 gtgctctcgg cgggatcacc gtgccggcgg ccgccgcact gctccgagcg ggtcggtgtg
     1201 ccacggcctg gccacccggc cgatgagccc tgctagaacc ggggcaggac cgcacccgta
     1261 aggggaggag caagcgacat gaccgacacc ggcctgctcg acgcactcgc ggaggtcctg
     1321 gccccggacc gggtgctcgg acccgaggtc gcctccgagg actacagcca cgacgagtgt
     1381 ctcaccgtcg cgtcccgtcg ccccgccgcg gtggtgctgg tcgagtcgac cgcggaggtg
     1441 tccgcggtgg tgaagctcgc ggccgagcgg aaggtcccga tcaccgcccg gggcagcggg
     1501 accgggctgt cgggcgccgc gatccccgcc gagggggcga tcgtcgtcag cttcgagcgg
     1561 atgaaccgcg tgctcgagct cgatcccgac aaccacgtcg cggtggtcca gcccggtctg
     1621 cggctcaacg agctcgacga ggtgaccgcc gcggccggtc tcgtctaccc cgtctacccc
     1681 ggcgagtact ccgccagcct gggaggcaac gtcgccacca acgccggggg catgcgggcg
     1741 gtgaagtacg gcgtcacccg ccaccaggtg ctcggactgg agctggtgct ggccgacggc
     1801 cagatcatcc gcaccggcgg gaagttcgtg aaggccacca ccggctacga cctgacccag
     1861 ctcgtcatcg gcagcgaggg caccctggcg ctggtcaccg aggcgaccct gcgcctctac
     1921 ccccgtcccg agcaccaggc cacggtcctc gccccgttca ccacgctgga gcaggtcacc
     1981 gcggcggtgc cgcgcctgat cgactcgggg gtcggtccgc tcatcctcga gtacatcgac
     2041 ctgctgacca tgtcggcgac cgccagcttc accggcctcg agctcggcat cccccaggag
     2101 atcaaggatt cggcactcgc ctacctggtg gtgatgctcg aggactccga tccgacgcgg
     2161 ctcgacgacg acatcgactc ggtggcccag cagctgcacg agctcggtgc catcgacgcc
     2221 tacgtgcttc cccccgctgc ggccgggcag ctgatcgacg cccgggagaa ggcgttctgg
     2281 atggccaagg ccaacggggc cgacgacgtc gtcgacatcg tcgtgccccg ggcgcagatc
     2341 cccgccttca tggagaaggt cgcggccctg gccggcgagt acgaggcctg ggtcgcggga
     2401 tgcggacacg cgggcgacgg caacgtccac ctggggatct tctgcgggga ccccgaccgt
     2461 cggtcccgtc tgctgcacgc cctcttcgcg gccggcgtcg ctctgggagg cgcgatctcg
     2521 ggcgagcacg ggatcggtga ggccaagcag gcctacttcc tggagctcga ggacccggcc
     2581 aagctggcgt tgatgcgccg gatcaaggcc gcgttcgacc ccgacgggat cctgaacccg
     2641 gggacactgc tcggctgagc gagccgtccg gcacccctca ccccgatccc acactcgaaa
     2701 ggcaccagcc atgaacggcg cacaggccat gatccgaacc ctcgtcgact gcggggtcga
     2761 cgtgtgcttc atgaacccgg gcacctccga gatgcacttc gtgcacgcgc tcgactcggt
     2821 gcccgagatg cggggagtcc tcgccctgtt cgaaggcgtc gccaccggcg ccgccgacgg
     2881 ctacgcccgg atggcagatc ggcccgccgc ggtcctgctg cacctcggtc cgggcctggg
     2941 taacggactg gccaacctcc acaacgcccg tcgggccaag gtcccggtcg tcaacgtggt
     3001 cggcgaccac gccacgtacc acgccaagta cgacgccccg ctgcagtccg acatcatctc
     3061 gatcgcccgc ggggtgtcgt cgtgggtccg caccgtcgcc tctcccgaga cgatcgcccg
     3121 ggacaccgca gacgccgtgg ccgcctccta cggcgccccg ggtcaggtcg cgacgctgat
     3181 cgtgccggcc gacacctgct ggttggactc gcccggcccg gtcggtccgt caccggtctc
     3241 cggagccggc accgtgccct ccgatcgcat cgacgacatc gccgccgtgc tgcgcggcgg
     3301 agagcccgcc gggctgctgg tcgggggcgc cgccatgcgc accggcgcgc tgcacgacgc
     3361 ggcgcgggtc gggatggcga ccggcgccaa ggtcctgagc gagaccttcc ccacccgcgc
     3421 cgagcggggc gcgggcaccc cggcggtcga gcgcctggcc tacctggccg agttcgccca
     3481 gatgcagctc cagggcctgc gtcacctggt gctggtcgac gccgcccatc cggcgtcgtt
     3541 cttcgcctac ccggacaagg cgtccgacct ggtcgccgag gggtgccagg tccacgtcct
     3601 gtcggccccc gacgaggacg cggcgggggc gctggccgcg ttggccgccg ccctcgacgc
     3661 gccgagcgac ccgccggtca tccc
//
LOCUS       DAOQOD010000253         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_11967_length_3673_cov_11.588447, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000253 DAOQOD010000000
VERSION     DAOQOD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11967_length_3673_cov_11.588447"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLZ72_15410"
     CDS             <1..642
                     /locus_tag="PLZ72_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37736.1"
                     /translation="FDDERAMVRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAV
                     RRRPYAVVLLDEVEKAHPDVFNVLLQLLDDGRLTDGQGRTVDFTNVVLIMTSNLPGDP
                     RDFFRPEFINRVDEMVRFRALEPGDLAAIVTIQLDHLRGRLAERRIDLVVTDDALAKL
                     AHDGYDAAFGARPLKRLIQQQVGDRLAMAILEGKLAEGDTVTVGVADGEFVLA"
     gene            723..1910
                     /locus_tag="PLZ72_15415"
     CDS             723..1910
                     /locus_tag="PLZ72_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37737.1"
                     /translation="MSELTGSVGGSEQVPRVPMGTLVRIGVLLTLVVVVIGSLNFARY
                     DWTGLPLNRAPMTATKDVSSRCVEHIGPYTTESGRVVAPVVVDEQQYMNLVELYRGVP
                     REDLQLTCLYDPFTFRSGTSWIAHLLPFEEGLSLGITNTVMTLLGLWLVLLALRAQGF
                     DARVVLAAGSVYAVSWNVFFFGTGVLVDSSVVAAIALCWWLLSTRRPWLVWPVLLLGY
                     PLKETVGIVLPAMAVWAWVEHREGRRSTAAAAGPVIAAAVAFVVGVAAWRGILPAPDA
                     AWPVKPNLQNTLHNIGDVVSLGSFAVGAGPFLVLGFLAYRREARRSGWVRAAVDPAVI
                     GVLTALGICFWSAITVDLTPRLFWIGTPFAATLVARWFSEGRPRAWLDGLARSNETGV
                     IAR"
     gene            1907..3019
                     /locus_tag="PLZ72_15420"
     CDS             1907..3019
                     /locus_tag="PLZ72_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37738.1"
                     /translation="MSSMRTFFARATTTPASRRFGVTWGAISSVLMWAVALPRFGAYE
                     EPPMEHLYSYVTAGGSTLRGLTVDMREYLTATEHFAGLRPAPSLGPFTQRVAAPWLAG
                     RLPWDAPISLNVVVMALLTIGLVALVATVLELKVSRCGLVVAAVGYAVSFPVFYWGSF
                     NYVDGAVVGLLACVLLFLVLERPFIAVGLVFVSMLFKESGLVGAFVVIAWVVAQASTD
                     FPRRQKVVWSATALASAVSGFVVARLVGPEATEVYNPWLITPAESFNYLGANIGRAGP
                     MAQVLLTGLLPLVLVVAAFRSSRRSELDAPRPVVWACVTGIAVGALLNVQALLSAQWD
                     GRTLWTVYPFVFALGAVALTRSVAARTFGTRRTSSL"
     gene            3043..>3673
                     /locus_tag="PLZ72_15425"
     CDS             3043..>3673
                     /locus_tag="PLZ72_15425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNA37739.1"
                     /translation="MPATVVVGTQWGDEGKGKLTDLVAKEMNSVVRYQGGHNAGHTLV
                     VDGESFALQLIPSGVLYDHITPIIGNGVVVDPVVMLSEVDALESRGVDCSRLRVSGNA
                     HLILPYHQELDRLIERHLGKNKLGTTKRGIGPTYADKASRVGLRVQDLLDADIFREKL
                     DAVLAEKNLVLAKIYNRLGFDPTELADRYLTDVAPRVAPYVADTVSLVHE"
BASE COUNT          483 a         1279 c         1264 g          647 t
ORIGIN      
        1 ttcgacgacg agcgggcgat ggtccgcatc gacatgagcg agtacatgga gaagcactcg
       61 gtcagccgcc tcatcggagc gcctcccggc tacgtcggct acgacgaggg cggccagctc
      121 accgaggccg tgcgccggcg tccgtacgcc gtcgtgctgc tcgacgaggt cgagaaggct
      181 caccccgacg tgttcaacgt gctgctgcag ctgctcgacg acggccggct caccgacggt
      241 cagggccgca cggtcgactt caccaacgtc gtgctgatca tgacctcgaa cctgcccggt
      301 gacccgcggg acttcttccg ccccgagttc atcaaccggg tcgacgagat ggtccggttc
      361 cgggctctgg agcccggcga cctggccgcc atcgtcacga tccagctcga ccacctccgt
      421 ggccgcctcg ccgagcgccg catcgacctg gtcgtcaccg acgacgccct ggccaagctc
      481 gcccacgacg gctacgacgc cgccttcggg gcccgtccgc tgaagcggct gatccagcag
      541 caggtcggcg accggctggc gatggcgatc ctcgagggca agctcgccga gggcgacacc
      601 gtcaccgtcg gcgtggccga cggcgagttc gtcctggcct gaccggaacc ggaccgcctc
      661 cggttgcctc ggcggctgcc gtccgataga cccgcgccga tgtgggcggc gcggggggcg
      721 gcgtgagcga actcaccgga tcggtcggcg gatcggagca ggtcccgcgc gtcccgatgg
      781 ggacgctggt gcgcatcggt gtgctgctca ccctcgtcgt ggtggtgatc ggctcgctga
      841 acttcgcccg ctacgactgg accgggctgc ccctcaaccg ggctccgatg accgccacca
      901 aggacgtcag ctcacgctgc gtcgagcaca tcgggcccta caccaccgag tcgggacgcg
      961 tcgtggcgcc ggtggtcgtc gacgagcagc agtacatgaa cctggtcgag ctctaccggg
     1021 gggtgccccg ggaggacctg cagctcacgt gcctctacga cccgttcacc ttcagatccg
     1081 ggacgtcgtg gatcgcgcac ctactgccct tcgaggaggg gctgtcgctc ggaatcacca
     1141 acacggtcat gaccctgctg gggctgtggc tcgtgctgct ggcccttcgc gctcagggct
     1201 tcgacgcccg cgtcgtgctg gctgccgggt cggtctacgc cgtgagctgg aacgtgttct
     1261 tcttcggcac cggcgtgctc gtcgactcct cggtggttgc cgccatcgcg ctctgctggt
     1321 ggctcctgtc gacgcgtcgc ccgtggttgg tgtggccggt gctgctcctc ggctacccgc
     1381 tcaaggagac cgtcggcatc gtgctcccgg cgatggcggt ctgggcctgg gtcgagcacc
     1441 gcgagggtcg acgatcgacc gccgcggcgg cgggacccgt catcgccgcc gccgtcgcgt
     1501 tcgtcgtcgg ggtcgcagcg tggcggggga tccttcccgc tcccgacgcc gcctggccgg
     1561 tgaagccgaa cctgcagaac acgctgcaca acatcggtga cgtggtcagc ctggggtcgt
     1621 tcgccgtcgg cgccggtccg ttcctcgtcc tcgggttcct cgcctaccgc cgcgaggccc
     1681 ggcgctccgg ctgggtccgg gccgcggtcg accccgcggt gatcggagtg ctcaccgcgc
     1741 tggggatctg cttctggtcg gcgatcaccg tcgacctcac cccccgcctg ttctggatcg
     1801 gcacgccgtt cgcggcgacc ctcgtcgccc gctggttctc cgaaggtcga ccgcgggcat
     1861 ggctggacgg actggccagg tcgaacgaga cgggtgtgat cgctcgatga gcagcatgcg
     1921 cactttcttc gcccgggcga cgactacgcc ggcaagccgc cggttcggcg tgacctgggg
     1981 agcgatctcg tcggtcctga tgtgggccgt ggcgcttccg cggttcggtg cctacgaaga
     2041 accgccgatg gaacacttgt actcctacgt caccgcgggc gggtccaccc tgcgcgggct
     2101 cacggtggac atgagggagt acctgacggc caccgagcac ttcgccgggt tgcgcccggc
     2161 gccgtccctc gggccgttca cccagcgtgt cgctgcgccg tggctggccg gccgcctccc
     2221 atgggacgcc ccgatctcat tgaacgtggt ggtgatggct ctgctgacca tcgggctcgt
     2281 cgctctggtg gcgaccgtgc tggagctgaa ggtgtccagg tgtggtctgg tcgtcgccgc
     2341 ggtcggctac gccgtttcgt tcccggtgtt ctactgggga tcgttcaact acgtcgacgg
     2401 tgccgtggtc ggattgctgg cgtgcgtgct gctgttcctg gtgctcgagc gacccttcat
     2461 cgccgtcggg ttggtattcg tgtcgatgct gttcaaggag tcggggctgg tcggggcgtt
     2521 cgtggtgatc gcgtgggtgg tcgcgcaggc gtcgacggac ttcccccggc ggcagaaggt
     2581 ggtgtggagt gccacagcgc tggcgagcgc cgtctcgggg ttcgtcgtgg cgcggttggt
     2641 cgggcccgag gccaccgagg tctacaaccc ctggttgatc acccccgcgg agagcttcaa
     2701 ctacctcggg gcgaacatcg ggcgggctgg tccgatggcc caggtgctgc tgacgggtct
     2761 cttaccactg gtgctcgtgg tcgccgcctt ccgatcctcc cggcgatccg agttggacgc
     2821 accgcgaccc gtcgtgtggg cctgcgtcac cgggatcgcc gtcggcgctt tgctgaacgt
     2881 ccaggccctg ttgagcgcgc agtgggacgg acggaccctc tggaccgtgt acccgttcgt
     2941 cttcgccctc ggcgccgtcg ccctcacccg gtcggtcgcc gcccgaacgt tcggcactcg
     3001 tcggacgagt agtctctgac gtcccctacc gaggagagag cagtgccggc gaccgtcgtg
     3061 gtgggcacac agtggggtga cgagggcaag ggcaagctga ccgacctcgt cgccaaggag
     3121 atgaactccg tcgtccgcta ccagggcggc cacaacgccg gccacacgct ggtggtcgac
     3181 ggcgagtcct tcgctctcca gctcatcccg tcgggggtcc tctacgacca catcaccccg
     3241 atcatcggca acggcgtggt cgtcgacccg gtcgtcatgc tgagcgaggt cgacgccctc
     3301 gagtcccgcg gcgtcgactg ctcgcgtctg cgggtgtccg gcaacgcgca cctcatcctg
     3361 ccgtaccacc aggagctcga ccggctgatc gaacgccacc tcggcaagaa caagctcggt
     3421 accaccaagc ggggcatcgg cccgacctac gccgacaagg cgtcgcgggt cgggttacgg
     3481 gtccaggacc tgctcgacgc cgacatcttc cgcgagaagc tcgacgcggt cctcgccgag
     3541 aagaacctgg tgctggccaa gatctacaac cgcctgggct tcgacccgac cgagctggcc
     3601 gaccgctacc tgaccgatgt cgcgccgcgg gtcgccccgt acgtggccga caccgtgtcg
     3661 ctggtgcacg agt
//
LOCUS       DAOQOD010000254         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_12070_length_3654_cov_11.454849, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000254 DAOQOD010000000
VERSION     DAOQOD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12070_length_3654_cov_11.454849"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1138
                     /locus_tag="PLZ72_15430"
     CDS             236..1138
                     /locus_tag="PLZ72_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37740.1"
                     /translation="MDQPTGLPDGLAAELAAAGLETTPASLGRWTGPCPVCPDGFVPT
                     VLVLDSADGIGLPPATVALLEAGFAVTPFDVAGVESQAVAGVPVRVGDVPGAAQAAVL
                     ASSVAEPVGLTDVQPVLVGSAGGLDAEQADEVAAIVSAAGLWVDSGSWTLSEAWNREQ
                     SLPIGGDAEPTLWPWSVVLVAVTLLVTAAHRREHGEAAAVLRVIGSTPRTTRRLASLT
                     AGSLAGVGVVMGLTLTIVVVGAAAAQRRYGSGWESARFDGLWNREATALLVVSLLIPV
                     VVALLARLIPPARPRRGWEGAAPA"
     gene            1163..2170
                     /locus_tag="PLZ72_15435"
     CDS             1163..2170
                     /locus_tag="PLZ72_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNA37741.1"
                     /translation="MRVLVTGATGFVGSHAVASMLAAGHDVRCLVRNPARLDGALSAT
                     DRERVDVAVGDVLDAASVRRALDGCDAVLHAAGAVGVSRAHSGSRDVNVEGTTHVIGQ
                     AAAAGCDPILYTSSIVVLDDSVPTMTAETPLGAPSGPYGRSKRDAELVARHHQAAGDP
                     VASFYVGGVFGPECPDVASGMSGIVGAVNQAMPIPHGGIGTIDVRDLARLLTAALEPG
                     RGPRRFMVGGQFLSWDDWSTLLGEVTGRPIRRIPMPSIVIRSSGRALDLLKRVRDFDY
                     PLTHEAAVQMTSSPRCDDRVTLAELGVDVRPVADTMADSVRWLIAEGHIDASRAPALA
                     P"
     gene            complement(2235..2993)
                     /locus_tag="PLZ72_15440"
     CDS             complement(2235..2993)
                     /locus_tag="PLZ72_15440"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNA37742.1"
                     /translation="MSNRTRTVSAVVVVAVLALIGGGLYWFLRDDAPDEVDLDTAAEG
                     VSTTGSTPGGSSSTAPGGAAPTGIDGTWTVDTSTGEFDYESATGTFVGFRVQEELASI
                     GAAEAVGRTNAVTGSMTIAGTEVTDATFTVDMTTITTNESRRDRRVQEALNTGQNPTA
                     TFTLTAPIDLGADAADGSEVTATAKGDLTINGVTRAVEFPLQAKLVDGTVVVVGSLDV
                     TFSDYGVTAPSAPIVLSVDDHGVLELQLLLTRTP"
     gene            3140..>3654
                     /locus_tag="PLZ72_15445"
     CDS             3140..>3654
                     /locus_tag="PLZ72_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNA37743.1"
                     /translation="MSIDFSLTPEIEEIRGRVRAFIDDVVKPTEARIQSEGLEHDDRD
                     AYLHALLDMRKQAYDAGLWLPHMPAEWGGMGLGHVELAMVQAEAAKSYYGPWVLNCQA
                     PDEGNMHTLLHWATDEQKQKYLKPLCDGRAWSCFAMTEPEVAGSDPTLIRTHAYQDGE
                     EWVINGHKWFIS"
BASE COUNT          479 a         1268 c         1377 g          530 t
ORIGIN      
        1 gtccccgtcc ccgaccacga tcccgggtga gccggggacg actccgacgg cggtgccgtc
       61 gagcgactcc ccgacggtcc cgaccgacgg accgaccggc caggtggtcg acaccgccgt
      121 cgtgtaccgc ccggcggagc gttcgtcatc ggcgtggtcc ggcacgtccg gctggacggt
      181 gagaggggag ggcgaaaacg gatcgggtga accggccccc ggcgctccca ccggagtgga
      241 tcagccgacc gggctccccg acggcctggc ggcggagctc gcggccgccg gcctggagac
      301 gacaccggcg tctctcggtc gttggaccgg gccctgtccg gtgtgtcccg acggcttcgt
      361 ccccacggtg ctcgtcctcg actccgcgga tgggatcggc ctgccgccgg cgacggtcgc
      421 cctgctcgag gccggcttcg ccgtcacccc cttcgacgtc gccggcgtcg agtcccaggc
      481 cgtggccggc gtgccggtcc gcgtcggtga cgtccccgga gcggcgcagg cggcggtgtt
      541 ggcgtcatcg gtcgccgagc cggtggggct caccgacgtc caaccggtgc tggtcggctc
      601 cgccggagga ctggacgccg agcaggccga cgaggtggcc gccatcgtgt ccgcggcggg
      661 gctctgggtc gactccggat cgtggaccct gtccgaggcg tggaaccggg agcagtccct
      721 gccgatcgga ggggacgcag agccgacgtt gtggccctgg tcggtggtgc tggtcgcagt
      781 cacgttgctg gtcaccgccg cgcaccgacg ggagcacggc gaggcagcag ccgtgctgcg
      841 ggtgatcgga tccacacccc ggaccacccg gcgcctggct tcgctgaccg cgggctcgct
      901 cgccggggtc ggtgtcgtca tgggactcac cctcaccatc gtcgtcgtcg gagccgccgc
      961 cgctcaacgc cggtacggat ctggttggga gagcgcgcgg ttcgacggcc tgtggaaccg
     1021 tgaggcgacc gcgctgctgg tcgtgtcgct gctcatcccg gtcgtggtcg cgctgctggc
     1081 ccggctgatc cctccggccc gcccacgccg aggctgggag ggcgcggcgc ctgcgtgacc
     1141 gcggtcgcta cgatcgccgg tcgtgcgagt tctggtgacc ggggcgacgg ggttcgtggg
     1201 atcgcatgcc gtcgcctcga tgctcgccgc aggccacgac gtgcgctgcc tcgtgcgcaa
     1261 ccccgcccgg ctcgacggcg cgctcagcgc gaccgaccgg gaacgggtcg acgtggcggt
     1321 cggcgacgtg ctcgacgccg cctcggtccg tcgggcgctc gacggctgcg acgccgtgct
     1381 gcacgccgcg ggcgctgtcg gggtgtcccg ggcgcactcg ggctcgcgcg acgtcaacgt
     1441 cgaggggacc acccacgtga tcggccaggc ggcggccgcg gggtgcgacc cgatcctgta
     1501 cacctcgtcg atcgtggtgc tcgacgactc ggtgccgacc atgaccgcgg agacgccgct
     1561 cggagcgccg tcgggtccgt acggtcgctc gaagcgcgac gccgagctcg tcgcacgcca
     1621 ccaccaggca gcgggtgacc cggtggcgag cttctacgtc ggcggggtgt tcgggcccga
     1681 gtgccccgac gtcgcgtcgg gcatgtcggg catcgtcggc gcggtcaacc aggcgatgcc
     1741 gatccctcac ggcggcatcg gcaccatcga cgtccgcgac ctggcccggc tgctgaccgc
     1801 cgcgctcgag ccggggcggg gaccgcgtcg gttcatggtc ggcgggcagt tcttgagttg
     1861 ggacgactgg tcgacgctgc tgggcgaggt cacgggtcga cccatccgtc gaatcccgat
     1921 gccgtcgatc gtgatccgct cgtcgggccg ggcactcgac ctgctcaagc gggtgcggga
     1981 cttcgactac ccgctcaccc acgaggccgc ggtgcagatg acgtcgtcac cgcgttgcga
     2041 cgaccgggtc accctcgccg agctcggcgt cgacgtccgt ccggtcgccg acacgatggc
     2101 cgacagcgtg cggtggctca tcgccgaggg tcacatcgac gcgtcccggg cgccggcact
     2161 cgccccctga cctgcccggc gggaacccgg gggcgcgcag ggcacacgag caggcagagc
     2221 ggtgagccct ccggtcaggg ggtgcgggtg aggagtagct gcagctccag cacgccgtgg
     2281 tcgtcgaccg acagcacgat cggcgcagag ggggcggtga cgccgtaatc ggagaacgtc
     2341 acgtcgagcg agccgacgac gaccacggtc ccgtcgacca acttggcctg cagcgggaac
     2401 tcgacggccc tggtcacccc gttgatggtc aggtctccct tggcggtcgc ggtgacctcg
     2461 gacccgtcgg ccgcgtcagc tccgaggtcg atgggggcgg tgagcgtgaa ggtggcggtc
     2521 gggttctgcc cggtgttgag cgcttcctgc acgcggcggt cccggcgtga ctcgttggtg
     2581 gtgatcgtgg tcatgtcgac ggtgaaggtc gcgtcggtca cctcggtgcc ggcgatggtc
     2641 atcgacccgg tcaccgcgtt ggtgcgaccg accgcctccg cggcaccgat cgaggccagc
     2701 tcctcctgca cccggaagcc gacgaaggtg ccggtcgccg actcgtagtc gaactcgccg
     2761 gtgctggtgt cgacggtcca ggtcccgtcg atgcccgtgg gcgccgcccc gcccggcgcg
     2821 gtgctcgacg atccgcccgg ggtggagccg gtggtcgaga cgccctccgc cgcggtgtcg
     2881 aggtcgacct cgtcgggggc gtcgtcgcgc aggaaccagt acagcccgcc gccgatgaga
     2941 gcgagcacgg cgacgacgac gacggcggag acggtcctgg tccggttcga catgcgggca
     3001 tcgtagggag tggtgccgga cggcggcggt cgggtggtcg tgaccttcga ccgcggcgac
     3061 ggtgggtggt ccggcgtggg gaggggtctg gtagaaccgg gccgatcggg gggaccgacc
     3121 gacccagggg gacgccgcag tgagcatcga cttcagcctg acaccggaga tcgaggagat
     3181 ccggggtcgg gtccgggcct tcatcgacga cgtggtcaag ccgaccgagg cccgcatcca
     3241 gtccgagggc ctcgagcacg acgaccgcga cgcctacctc cacgcgctgc tcgacatgcg
     3301 caagcaggcc tacgacgctg ggctgtggct cccgcacatg ccggccgagt ggggcggcat
     3361 ggggctcggc cacgtcgaac tggccatggt gcaggccgaa gcggccaaga gctactacgg
     3421 cccgtgggtc ctcaactgcc aggcgccgga cgagggcaac atgcacacgt tgctgcactg
     3481 ggcgaccgac gagcagaagc agaagtacct caagccgctg tgcgacggtc gggcgtggtc
     3541 gtgcttcgcc atgaccgaac ccgaggtcgc cgggtcggat ccgaccctca tccgcaccca
     3601 cgcctaccag gacggcgagg agtgggtgat caacggccac aagtggttca tctc
//
LOCUS       DAOQOD010000255         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_12142_length_3644_cov_9.134021, whole genome shotgun sequence.
ACCESSION   DAOQOD010000255 DAOQOD010000000
VERSION     DAOQOD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12142_length_3644_cov_9.134021"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLZ72_15450"
     CDS             <1..1133
                     /locus_tag="PLZ72_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNA37744.1"
                     /translation="RALYLGGPGHEVLRRLGIRPRGHKPPTRGSRVSLEGRIDDLPVG
                     LASLGRSGVVPGRAKPTLVAALAALMAGRPASARPDEPASAWIRDQSRGRDDVAALVA
                     ALFRVATYTADLDQLSAAAAVPHVARSLRRGVLYLDGGFEQLLDSLRSVATGADAPVA
                     VHRHVAVSRLEQTVDGGWEVQCRDGRTVRAGAVVVAAGGPAVAADLLDLPELVDRSGP
                     PATAACLELAVAGPVPTRFVLGVDEPLYLSEHSPPADLAPDGVRVVHVARYGATGAVA
                     DRARLESLASAAGIDPGDVVARRFLRRMVVQTAIPSVDHGGLVGRPRVAVADRPGVFL
                     AGDWVGPVGMLADASLASGEAAGSLAAMAVVGGAATVRSVVA"
     gene            1130..2053
                     /gene="sigJ"
                     /locus_tag="PLZ72_15455"
     CDS             1130..2053
                     /gene="sigJ"
                     /locus_tag="PLZ72_15455"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HNA37745.1"
                     /translation="MSGSEVSGVEVFEAERARLTGLAYRMLGSISDAEDVVSEAWLRY
                     QRADPAMLERPEAWLTTVTSRLAIDRLRAITRRREEYVGPWLPEPVPTGDAPAGTGLS
                     VTPAPAEDPADRVALSESLTLGFLVVLDRLGPVERVVFLLADVFGEPYARIADTVGRS
                     EEACRQIASRARRRVREERADRAAADESLLADLVGALVLGDVDAVLGMLSPDVVLTSD
                     GGPDRHAARRPVIGPDRVARLLINLAGRTPEDADISIETVNAAPALVVRHPELPVVVA
                     ADRDAEGRVIAVRLLLNPVKLGRLDASFPGI"
     gene            complement(2102..3565)
                     /locus_tag="PLZ72_15460"
     CDS             complement(2102..3565)
                     /locus_tag="PLZ72_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNA37746.1"
                     /translation="MRSFDRLYIGGEWVEPASSSTFTVVNPATLEPVGSTPEAVQADV
                     DRAVEAARSALNHGPWAEMSAQDRAGIMRRLHGLMVERADDLANLITQEVGAPILFSH
                     FGQIGATHMALDFFTGLTESYPFEEVRAGMLGPVVVRKEPVGVVAGIVPWNVPLFITM
                     LKFAPTLASGSTMVLKPAPETPVSAFVFAELCEEAGVPAGVLNVVPADREVGEHLVTH
                     PGVDKVSFTGSTAAGRHIGALCGERLRRCTLELGGKSASILMEDADIAAAVTEMVPNY
                     SFMNNGQACVAQTRILAPRSRYDETVDAVTEVARSVKVGDPADFEVGVGPLIAERQRE
                     RVEGYIRTGIDEGARLTTGGGRPAGLDTGWYVEPTVFADVDNKMRIAQEEIFGPVVVV
                     IPYTDTEDALAIANDNNYGLSGSVWTADVAAGLDVARGVRTGTYTVNGFGMDWGSPFG
                     GFKDSGLGRELGPEGLEEYLEYKSINLPAGTEPELRF"
BASE COUNT          440 a         1285 c         1391 g          528 t
ORIGIN      
        1 cgcgggcgtt gtacctcggc ggtcccggcc acgaggtgct ccgccgactg gggatccgac
       61 cgcggggaca caagcccccg acgcgtggca gcagggtcag ccttgaggga cggatcgacg
      121 acctgccggt cggcctcgcc tcgctgggcc gaagcggggt cgtcccgggc cgggccaagc
      181 cgacgctggt cgccgccctc gccgcgctga tggcgggtcg tcccgcgtcg gcccggcccg
      241 acgaacccgc ctccgcgtgg atccgggacc agtcacgggg tcgggacgac gtcgccgcgt
      301 tggtggcggc cctgttccgg gtcgccacct acaccgcaga cctggaccag ctgtcggccg
      361 ccgcggcggt cccgcacgtc gctcgaagcc tccgccgggg cgtgctctac ctcgacggag
      421 gcttcgagca gctgctcgac tcgctccgat ccgtcgcgac gggcgcggat gctccggtgg
      481 cggtccatcg gcacgtcgcg gtgtcacgcc tggagcagac cgtggacggc gggtgggagg
      541 tgcagtgccg cgacggacgg acggtgcgtg cgggtgcggt ggtggtcgcc gccggcggcc
      601 ccgcggtggc cgcggacctg ctggacctgc cggagctggt cgaccgctct ggcccgccgg
      661 cgaccgctgc ctgcctggag ctcgcggtgg caggtccggt gccgacccgt ttcgtcctcg
      721 gcgtcgacga accgctgtac ctctcggagc actcgccgcc ggcggacctc gcgcccgacg
      781 gggtcagggt ggtgcacgtg gctcgctacg gagccaccgg cgccgtggcc gatcgggccc
      841 ggctggagtc gctggcgtcc gccgccggca tcgaccccgg tgacgtcgtc gcccgccggt
      901 tcctgcgtcg gatggtcgtg cagaccgcga ttccctccgt cgaccacgga gggctggtcg
      961 gacgaccccg cgtcgccgtg gccgaccgac cgggggtctt cctcgccggc gactgggtgg
     1021 gtccggtggg gatgctggcg gacgcgtcgc tggccagcgg tgaggccgcg gggtccctcg
     1081 cggccatggc ggtcgtgggc ggggcggcta ccgtccggtc ggtggtcgcg tgagcgggtc
     1141 ggaggtgtcc ggcgtcgagg tcttcgaggc ggagcgcgcc cgactgaccg ggctggcgta
     1201 ccggatgctc ggatcgatct ccgacgccga ggacgtggtg tccgaggcct ggctgcgcta
     1261 ccagcgggcc gatccggcga tgctggagcg gcccgaggcg tggttgacca ccgtgacctc
     1321 gcggctggcg atcgaccggt tgagggcgat cacccgccgg cgcgaggagt acgtcgggcc
     1381 gtggctgccg gagccggtcc ccaccggcga tgccccggcc gggaccgggc tgtcggtcac
     1441 cccggccccg gccgaggacc ccgcggaccg ggtggcgctg tcggagtcgc tcacactcgg
     1501 attcctggtg gtgctcgacc ggctgggacc ggtggagcgg gtggtgttcc tgctcgcgga
     1561 cgtcttcggt gagccgtacg cccgcatcgc cgacacggtc ggccggtccg aggaggcgtg
     1621 ccgtcagatc gcgtcccggg cccgacgccg tgtgcgcgag gagcgcgccg accgggccgc
     1681 ggcggacgag tcgctgctcg ccgatctggt cggagctctc gtcctcggtg acgtcgacgc
     1741 cgtgctcggc atgctgtcac cggacgtcgt gctgaccagc gacggcggac cggatcggca
     1801 cgcggcgcgc cgtccggtga tcgggccgga ccgggtcgcc cggctcctga tcaacctcgc
     1861 cgggaggacg ccagaggacg ccgacatctc gatcgagacc gtcaacgccg ctcctgcgct
     1921 cgtggtccgc caccccgagc tcccggtggt ggtggctgcc gaccgcgatg ccgagggccg
     1981 ggtcatcgct gtgcggctgc tcctcaaccc ggtcaagctc ggacggctgg acgcgtcctt
     2041 cccgggcatc tgacggcggc ggaccgtccg gacggcccgg gcgtgtccgc cgccgtcggg
     2101 atcagaaacg gagctcgggc tcggtgccgg ccggcaggtt gatcgacttg tactccaggt
     2161 actcctcgag gccctcgggt cccagctcgc ggccgaggcc cgagtccttg aacccgccga
     2221 agggcgagcc ccagtccatg ccgaacccgt tgacggtgta cgtgccggtg cgcacgcctc
     2281 gggcgacgtc gagtccggcg gccacgtccg cggtccacac cgagcccgac agtccgtagt
     2341 tgttgtcgtt ggcgatggcg agggcgtcct cggtgtcggt gtaggggatg acgaccacga
     2401 ccgggccgaa gatctcctcc tgggcgatgc gcatcttgtt gtcgacgtcc gcgaacaccg
     2461 tcggctccac gtaccagccg gtgtcgaggc cggccgggcg acctccgccg gtggtgaggc
     2521 gggcgccctc gtcgatgccg gtgcggatgt agccctcgac ccgttcgcgc tggcgctcgg
     2581 cgatcagcgg gccgacgccg acctcgaagt cggccgggtc gccgaccttg accgagcggg
     2641 ccacctcggt gaccgcgtcg acggtctcgt cgtagcgcga tcggggggcg aggatgcggg
     2701 tctgcgcgac gcaggcctgg ccgttgttca tgaacgagta gttgggcacc atctcggtga
     2761 ccgccgcggc gatgtcggcg tcctccatga ggatcgacgc cgacttgccg ccgagctcca
     2821 gggtgcaccg gcggaggcgt tcaccgcaca gcgcgccgat gtggcgcccc gccgcggtcg
     2881 acccggtgaa ggagaccttg tcgaccccgg ggtgggtgac caggtgctcg ccgacctcgc
     2941 ggtcggcggg gacgacgttc agaacgccgg cggggacccc ggcctcctcg cacagctccg
     3001 cgaacacgaa cgcgctgacc ggtgtctcgg gagcgggctt gagcaccatc gtcgaacccg
     3061 acgcgagcgt gggggcgaac ttcaacatgg tgatgaacag cggcacgttc cacgggacga
     3121 tgccggcgac gacgccgacc ggttccttgc gaaccacgac cgggccgagc atcccggccc
     3181 gcacctcctc gaacgggtac gactcggtga gcccggtgaa gaagtcgagc gccatgtggg
     3241 tggcgccgat ctggccgaag tgcgagaaca ggatgggcgc gccgacctcc tgggtgatca
     3301 ggttggccag gtcgtcggcc cgctcgacca tgagcccgtg gagccggcgc atgatcccgg
     3361 cccggtcctg cgcggacatc tcggcccacg ggccgtggtt cagagcggac cgggcggcct
     3421 cgacggcccg gtcgacgtcc gcctggacgg cctccggcgt ggagccgacc ggctcgagcg
     3481 tggccgggtt gacgacggtg aaggtgctcg acgacgcggg ctccacccac tcgccgccga
     3541 tgtagagccg gtcgaaggaa cgcacgttcc atccccctga ggtcgggccg gtgccgcggg
     3601 gcgccgacgc gtcggatgcc gcgcagcgta tcccgccggc atcg
//
LOCUS       DAOQOD010000256         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_12155_length_3641_cov_11.575851, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000256 DAOQOD010000000
VERSION     DAOQOD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12155_length_3641_cov_11.575851"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLZ72_15465"
     CDS             <1..703
                     /locus_tag="PLZ72_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37747.1"
                     /translation="APSAAPDDNGGHGGHIGPTPWAAMTVQSQCVALAEELATARKAA
                     LDLPTAADAMAAGYVRVTPYVPGIGAHYMKFSLVDGEFAVDEPEMVLYDGNGPDAHVV
                     GLSYYVVQPGDAEPTQGFTGDNDHYHRHVGLCTAPDAGVIGDSSTTAEECAARGGVKA
                     NGSAGWMSHAWVVPGCESPWGVFSGATPILDRELGEASGRNDGGCSASSGRDRYDLSA
                     GGRSPSERGEQAAGG"
     gene            complement(718..1797)
                     /locus_tag="PLZ72_15470"
     CDS             complement(718..1797)
                     /locus_tag="PLZ72_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNA37748.1"
                     /translation="MSGPADAEPVFAEQLGSDDPLERARAALDLALYYLDRGFAPAGK
                     TRAFLKARDIVGEIGSEDLRSRVAAGTLTDLAGIGPSTGSVISDAVDRRPSRYLADLA
                     SATVQDVGSGGGIRSLLRGDLHSHTFWSDGSESVATMARSARALGHEYLAVTDHSARL
                     TVAHGLDEARLGEQLTEIEALNAELAPFRILTGMEVDILEDGRLDLSSEMLSRLDVVV
                     ASVHSKLAMDEREMTRRMVMAVADPDVDVLGHCTGRKITGRGRPPSRFDADIVFAACA
                     RFDTAVEINCRPERRDPPEELTALALEWGCRVSIDTDAHAPGQLEWQAWGCGFAADMG
                     IEPERIINTYPADELLGWTAAHPTA"
     gene            1849..2937
                     /gene="ychF"
                     /locus_tag="PLZ72_15475"
     CDS             1849..2937
                     /gene="ychF"
                     /locus_tag="PLZ72_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNA37749.1"
                     /translation="MERFGFVGLPNAGKSSLYNALAGGGALAAPYAFATVDPNVGVAK
                     VPDPRLYALAEMSRTKTIIPAAVQFVDIAGLVKGAAQGEGLGNRFLSHIREVDAIVFV
                     LRAFVDDDVPGSTDPVEQLETLEIELVYADLESLEKQIDKRRKAARADRTLADEVAAL
                     EEAIEHLQAGTPLYRAGLDEERAALLKPFFLLTAKPAMALVNVDEAQMEDLDEVLAPV
                     RTAFAATGAADELVLGLCIQLEAEAALLDEESRVEMLDALGLGEGALPQFLHTAYSLL
                     GLRTYFTTGEKETRAWTFRAGSTAPQTAGVIHTDFERGFIRAEVIHWDELLECGSWAA
                     ARDVGKLRVEGKDYRPVDGDVMEFRFNV"
     gene            2950..3294
                     /locus_tag="PLZ72_15480"
     CDS             2950..3294
                     /locus_tag="PLZ72_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNA37750.1"
                     /translation="MPWLVRNGDVLASVETAATHRERRRGLIGRDPLDGVLRMRARSV
                     HTFGMRVPIDVVTCIPLGAGGYRVVRVLTVAPRRVTRPSLTASVVFEAEAGSFRRWDV
                     RAGDRLEVRGDG"
     gene            3287..>3641
                     /locus_tag="PLZ72_15485"
     CDS             3287..>3641
                     /locus_tag="PLZ72_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNA37751.1"
                     /translation="MAETPTPDGRVVVVGTPIGNLGDLSPRAVAALEGADVVACEDTR
                     RSGRLLQHAGVPGKRLVRLDDHTEAAVTADLVARAAAGDRVALVSDAGLPGLSDPGAV
                     LLAAAWSAGVAVEVVP"
BASE COUNT          494 a         1303 c         1352 g          492 t
ORIGIN      
        1 ggctccgagc gcggcgcccg acgacaacgg cggtcacggt gggcacatcg gtccgacgcc
       61 gtgggccgcc atgacggtcc aatcccagtg cgtggcgctg gccgaggagc tcgcgacggc
      121 ccgcaaggcc gccctcgacc tgccgaccgc ggccgacgcc atggctgcgg gctacgtccg
      181 ggtgacgccc tacgtgccgg gcatcggggc ccactacatg aagttctcac tggtcgacgg
      241 ggagttcgcc gtcgacgagc ccgagatggt cctctacgac ggcaacggtc ccgacgccca
      301 cgtggtcggc ctcagctact acgtggtgca gcccggcgac gccgagccga cccagggctt
      361 caccggcgac aacgaccact accaccgcca cgtcgggttg tgcaccgcac cagacgccgg
      421 ggtgatcggc gactcgtcga ccaccgccga ggagtgcgcg gcacgcggcg gggtgaaggc
      481 caacgggtcg gcgggctgga tgtcgcacgc ctgggtggtc cccggctgcg agagcccgtg
      541 gggcgtcttc agcggggcga caccgatcct cgaccgcgag ctcggcgagg cgtcgggccg
      601 caacgacggc ggatgctcgg catcgagcgg ccgggaccgc tacgacctca gcgccggtgg
      661 tcgatcaccg tcggagcggg gcgagcaggc cgcggggggc tgagatcacc cgaccggtca
      721 cgcggtcggg tgcgcggcgg tccacccgag cagctcgtcg gccggatagg tgttgatgat
      781 ccgctccggc tcgatgccca tgtcggcggc gaagccgcac ccccacgcct gccactcgag
      841 ctgaccgggg gcgtgggcgt cggtgtcgat cgacacccgg cacccccact ccagcgccag
      901 cgccgtcagc tcctcgggcg ggtcccggcg ctccggacgg cagttgatct cgaccgcggt
      961 gtcgaaccgg gcgcaggccg cgaacacgat gtcggcgtcg aagcgcgacg gagggcgacc
     1021 ccgcccggtg atcttgcggc cggtgcagtg gccgagcacg tcgacgtcgg gatcggcgac
     1081 ggccatcacc atccgtcggg tcatctcccg ctcgtccatc gccagcttcg agtggaccga
     1141 cgccacgacc acgtcgagcc gcgacagcat ctccgaggac aggtcgaggc gcccgtcctc
     1201 gaggatgtcg acctccatgc cggtcaggat ccggaacggg gcgagctcgg cgttgagcgc
     1261 ctcgatctcg gtgagctgct cgcccaggcg ggcctcgtcc agtccgtggg ccacggtgag
     1321 tcgggccgag tggtcggtca ccgccaggta ctcgtggccg agcgcccggg ccgagcgcgc
     1381 catggtcgcc acgctctcgg agccgtcgga ccagaacgtg tggctgtgga ggtcgcccct
     1441 gagcagggag cggatcccgc cgccggaccc gacgtcctgg acggtggccg aggcgaggtc
     1501 ggccaggtaa cgcgacggcc gccggtcgac cgcgtcggag atcaccgagc cggtcgacgg
     1561 gccgatcccg gccaggtcgg tgagggttcc ggcggcgacc ctggagcgga ggtcctcgga
     1621 gccgatctca ccgacgatgt cccgtgcctt gaggaacgcc cgggtcttgc cggccggagc
     1681 gaacccgcgg tccaggtagt agagcgccag gtcgagcgcg gcccgggcgc gttcgagcgg
     1741 gtcgtcggag ccgagctgct cggcgaagac cggttcggcg tcggcgggcc cgctcacggt
     1801 cgaccggtct acacaccgcg tccgaggtct gccactaccc tccgcaacgt ggaacggttc
     1861 ggattcgtcg gactgccgaa cgccggcaag tcgtccctgt acaacgccct ggccggcggc
     1921 ggcgccctgg cggcgccgta cgccttcgcg acggtcgacc ccaacgtcgg tgtggccaag
     1981 gtgcccgacc cccggctgta cgcgctggcc gagatgagcc ggaccaagac gatcatcccc
     2041 gccgcggtgc agttcgtcga catcgcgggc ctggtcaagg gggcggccca gggcgagggc
     2101 ctgggcaacc ggttcctctc ccacatccgc gaggtcgacg ccatcgtgtt cgtcctgcgg
     2161 gcgttcgtcg acgacgacgt ccccggttcc accgaccccg tcgagcagct cgagaccctc
     2221 gagatcgagc tggtctacgc ggacctggag tcgctcgaga agcagatcga caagcgccgc
     2281 aaggccgccc gcgccgatcg gaccctggcc gacgaggtcg ccgccctcga agaggcgatc
     2341 gagcacctgc aggccggaac cccgctgtac cgcgccggac tcgacgagga acgcgcggcg
     2401 ctgctcaagc cgttcttcct gctgaccgcc aagccggcga tggcgctggt caacgtcgac
     2461 gaggcccaga tggaggacct cgacgaggtg ctcgccccgg tgcggaccgc cttcgccgcc
     2521 accggcgccg ccgacgagct ggtgctcggt ctgtgcatcc agctcgaggc cgaggccgcg
     2581 ctgctcgacg aggagtcccg tgtcgagatg ctggacgcgc tgggcctggg ggagggggcg
     2641 ctgccgcagt tcctccacac cgcgtactcg ctgctcgggc tgcgcaccta cttcaccacc
     2701 ggcgagaagg agacccgggc gtggaccttc cgggccgggt cgaccgcacc gcagaccgcc
     2761 ggggtcatcc acaccgactt cgaacgcggc ttcatccgcg ccgaggtcat ccactgggac
     2821 gagctgctcg agtgcgggtc gtgggccgcg gcgcgtgacg tgggcaagct gcgggtcgag
     2881 ggcaaggact accgcccggt cgacggcgac gtcatggagt tccgtttcaa cgtctgagga
     2941 gtggcgcagg tgccgtggtt ggtgcgcaac ggtgacgtcc tggcgtcggt cgagaccgcc
     3001 gccacgcacc gcgagcgtcg ccgcggactg atcggtcgcg acccgctcga cggcgtcctg
     3061 cggatgcggg cccggtcggt gcacaccttc ggcatgaggg tgccgatcga cgtcgtcacc
     3121 tgcatcccac tgggcgcggg cggctaccgg gtggtgcggg tcctcaccgt cgcgccacgg
     3181 cgggtcaccc ggccctccct gaccgcgtcg gtggtgttcg aagccgaggc cggctcgttc
     3241 cggcgctggg acgtccgcgc cggcgaccgc ctcgaggtgc gcggcgatgg ctgagacccc
     3301 gacgccggac gggcgcgtgg tcgtggtcgg cactccgatc ggcaacctgg gcgacctgtc
     3361 gccccgggcg gtcgcggcgc tcgagggggc cgatgtggtg gcgtgcgagg acacccgtcg
     3421 gtcgggccgg ttgctgcagc acgccggcgt ccccggcaag cggctggtcc gcctcgacga
     3481 ccacaccgag gcggcggtga ccgccgacct cgtcgcccgg gccgcggccg gcgaccgcgt
     3541 ggcgctggtc agcgacgccg gcctgcccgg gttgtcggat ccgggcgcgg tcctcctggc
     3601 cgcggcgtgg agcgccggcg tcgcggtcga ggtcgtgccc g
//
LOCUS       DAOQOD010000257         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_12895_length_3517_cov_10.426054, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000257 DAOQOD010000000
VERSION     DAOQOD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12895_length_3517_cov_10.426054"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2641)
                     /locus_tag="PLZ72_15490"
     CDS             complement(<1..2641)
                     /locus_tag="PLZ72_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNA37752.1"
                     /translation="MTNRRPGERPGPVGLYRPESEHDACGVSFVCDLHGRPSHDLVAK
                     GLTSLCNLDHRGATGAETNTGDGAGILVQVPDAFFRAVLPFDLPERGAYAVGTAFLPA
                     DDAAADAAAAAVEKICAEEGAEVLGWRTVPVDPSGIGSTALDAMPTFRQLFLADVDRN
                     RHDIALDRLAYVVRKRVEHEVLDPDGAAAVYFPSLSCRTLVYKGMLTTPQLGDFFDDL
                     RDERFESALALVHSRFSTNTFPSWPLAHPYRYLAHNGEINTVQGNQNWMRAREALLSS
                     PHLPGGEDLERIFPICTPGSSDTARLDEVLELLHLGGYSLPHAMLMMIPEAWERHTDM
                     DPAVRDFYRFHSCIMEPWDGPASVTFTDGTVVGAVLDRNGLRPSRYWVCDDGLVVMAS
                     EVGTLPIDVGRVVRKGRLQPGRMFLIDTAQGRIIDDAEIKAELAAAHPYGEWMADKLR
                     RLEDLPEVAHIRESRRDVLREQQTFGYTQEDLKILIEPMVRTGGEAIGSMGTDTPLAV
                     LSERPRLVYDYFQQLFAQVTNPPLDAIREELVTSLSKVIGPESNLLDPGPESCHLLEM
                     PHPILTNEDLDRIQHIEEADPHFKARTVRCLFPAFSGGRGLARALERIRSEVSEAIAE
                     GANVIILSDWLSNEDLAPIPMLLATSAVHHHLIREKTRTQVGLVVETGEAREVHHFAL
                     LLGFGAAAVNPYLAFDSIEHMVAEGTTTITDFDAAVANYRKAVGKGILKVMSKMGIST
                     VASYTGAQIFEAIGLGADLVDEYFTGTVSRLGGIGLDEVAEEVRRRHARAYPFRQEER
                     AHRTLESGGEYQWRREGEYHLFNPETVYKLQHSTRSGQYEVFKEYTQLVNDQAENLAT
                     LRGLFALRTGVRPAVPLDEVEPAS"
     gene            2993..>3517
                     /locus_tag="PLZ72_15495"
     CDS             2993..>3517
                     /locus_tag="PLZ72_15495"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNA37753.1"
                     /translation="MRPRRLHRTVRGLLVLGAVALAAAACAGTDESSVAGRTPGSDRS
                     GDAEAMSDTGASRDTDATTTTTTRPPTGSGQAVTLAFAGDTNFNNQLGALRSSPGTVL
                     SAIAPTLSAADVTMLNLEAALGTGGSPQPKKFTFQVPAQAIDAVKAAGVDVVTMANNH
                     GMDFGPVGFADSLRI"
BASE COUNT          493 a         1176 c         1325 g          523 t
ORIGIN      
        1 cggacgcggg ttcgacctcg tcgaggggaa ccgcggggcg gaccccggtg cgcagggcga
       61 acagcccgcg gagcgtggcg aggttctcgg cctggtcgtt gaccagctgc gtgtactcct
      121 tgaacacctc gtactggccg ctgcgggtcg agtgctgcag cttgtagacg gtctccgggt
      181 tgaacaggtg gtactcgcct tcgcggcgcc actggtactc gccgccggac tcgagggtgc
      241 ggtgcgcccg ctcctcctga cggaacgggt aggcgcgggc gtggcgacgg cggacctctt
      301 cggccacctc gtccaggccg atgccgccca ggcggctgac cgtgccggtg aagtactcgt
      361 cgaccagatc ggcaccgagg ccgatcgcct cgaagatctg ggcgccggtg taggacgcca
      421 cggtcgagat gcccatcttg gacatcacct tgaggatccc cttgccgacc gctttgcggt
      481 agttggcgac cgcggcgtcg aagtcggtga tcgtcgtggt gccctcggcg accatgtgct
      541 cgatcgagtc gaacgccaga taggggttga ccgccgcggc gccgaagccg agcaggaggg
      601 cgaagtggtg cacctcgcgg gcctcgccgg tctcgaccac caggccgacc tgggtgcggg
      661 tcttctcgcg gatgaggtgg tggtgcaccg ccgaggtggc cagcagcatc gggatcgggg
      721 ccaggtcctc gttggagagc cagtccgaca ggatgatgac gttggcgccc tcggcgatcg
      781 cctcggacac ctcggagcgg atccgctcga gcgcccgggc cagaccccgg ccgccggaga
      841 acgcggggaa caggcagcgc acggtgcggg ccttgaagtg cgggtcggcc tcctcgatgt
      901 gctggatccg gtccaggtcc tcgttggtga ggatcgggtg cggcatctcg agcaggtggc
      961 aggactcggg tcccggatcg agcaggttgc tctcggggcc gatgaccttg ctgagcgagg
     1021 tgaccagctc ctcgcggatg gcgtcgagcg gcgggttcgt cacctgggcg aacagctgct
     1081 ggaagtagtc gtagaccaac cgcgggcgct cggagaggac cgccagcggc gtgtcggtgc
     1141 ccatcgagcc gatcgcctct ccgccggtgc ggaccatcgg ctcgatcagg atcttgaggt
     1201 cctcctgggt gtagccgaag gtctgctgct cgcgcagcac gtcgcggcgg gactcgcgga
     1261 tgtgggcgac ctcgggcagg tcctcgagcc ggcgcagctt gtccgccatc cactcgccgt
     1321 aggggtgcgc cgcggccagc tcggccttga tctccgcgtc gtcgatgatc cggccctggg
     1381 cggtgtcgat caggaacatc cgcccgggtt ggagccggcc cttgcgcacg acgcggccga
     1441 cgtcgatcgg cagcgtgccg acctcggagg ccatgaccac caggccgtcg tcgcagaccc
     1501 agtagcgcga cgggcgcagg ccgttgcggt cgagcacggc gccgacaacg gttccgtcgg
     1561 tgaaggtgac cgaggcgggg ccgtcccagg gctccatgat gcaggagtgg aaccggtaga
     1621 agtcccggac cgccgggtcc atgtcggtgt gccgctccca cgcctcgggg atcatcatca
     1681 gcatggcgtg cggcagcgag tacccgccca ggtgcagcag ctcgagcacc tcgtcgagtc
     1741 gggctgtgtc ggagctgccg ggggtgcaga tcgggaagat gcgctccagg tcctcgccgc
     1801 cgggcaggtg cggtgaggac agcagcgcct cgcgggcccg catccagttc tggttgccct
     1861 ggacggtgtt gatctcgccg ttgtgggcca ggtagcggta cgggtgcgcc agcggccacg
     1921 acgggaacgt gttggtcgag aaccgggagt gcaccagggc cagggccgac tcgaagcgct
     1981 cgtcgcgcag gtcgtcgaag aagtcgccga gctgcggggt ggtgagcatc cccttgtaga
     2041 ccagcgtccg acacgacagc gacgggaagt acaccgcggc ggcgccgtcg gggtcgagca
     2101 cctcgtgctc gacccgcttg cgcacgacgt aggcgagccg gtccagggcg atgtcatggc
     2161 ggttgcggtc gacgtcggcc aggaacagct ggcggaacgt cggcatggcg tcgagcgcgg
     2221 tggagccgat gcccgacggg tcgaccggga cggtgcgcca cccgagcacc tcggcgccct
     2281 cctcggcgca gatcttctcg accgcggcgg cggccgcatc cgcggcggcg tcgtccgcgg
     2341 gcaggaacgc cgtcccgacg gcgtaggcac cccgttcggg caggtcgaac ggcaggaccg
     2401 cgcggaagaa ggcgtcgggg acctgcacca ggatgccggc gccgtcgccg gtgttcgtct
     2461 ctgccccggt cgctccccgg tggtcgaggt tgcacagcga ggtgaggccc ttggcgacca
     2521 ggtcgtggct ggggcggccg tggaggtcgc acacgaagga gacgccgcag gcgtcgtgct
     2581 cggattcggg gcggtagagg cccaccggtc cggggcgctc cccgggtcgt cggttggtca
     2641 tggtgctcct gatgcagtgc gtccggggac cggacgtcgg tctgggtgcg gggcgtccca
     2701 cccgggttcg ccccgagcag tcgacgctga ctgcaacagc tgcggatcgc cgcgagcgtg
     2761 tgggccggcg acatcggcgg cccggtccgg gccgtcgttg gcctcggaac ctgccgtggg
     2821 tgcccgacga acgggtccca cagggggtcg gtccacccta gccgagctcg tggggacccc
     2881 ccaacgtgaa ttccgttccc gctgttcccg tgtggatcag gcgcgtgggg cgtcgttctc
     2941 acacgcggag cgggtcgcac ggcacggccg gacggcccgg taccgtggcc cgatgcgccc
     3001 ccgacgactc caccgaaccg tgcgcgggct gctggtcctg ggtgccgtcg cgctggccgc
     3061 ggcggcgtgc gcgggcaccg acgagtcgtc ggtcgccggc aggacgccgg ggagcgaccg
     3121 ctccggcgac gccgaggcga tgagcgacac cggggcgagc agggacacgg acgcgaccac
     3181 cacgaccacc actcggcccc cgaccggcag cggccaggcg gtgaccctgg ccttcgccgg
     3241 tgacaccaac ttcaacaacc agctcggagc gctccgctcc tctccgggga ccgtcctgtc
     3301 ggccatcgcc cccacgctgt cggcggcgga cgtgaccatg ctcaacctgg aggccgcgct
     3361 cgggaccggc ggcagccccc agcccaagaa gttcacgttc caggtgccgg cccaggccat
     3421 cgacgcggtg aaggcggccg gggtcgacgt cgtcaccatg gccaacaacc acggcatgga
     3481 cttcggtccc gtcgggttcg ccgactcgtt gcggatc
//
LOCUS       DAOQOD010000258         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_12918_length_3514_cov_9.900549, whole genome shotgun sequence.
ACCESSION   DAOQOD010000258 DAOQOD010000000
VERSION     DAOQOD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12918_length_3514_cov_9.900549"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLZ72_15500"
     CDS             complement(<1..718)
                     /locus_tag="PLZ72_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37754.1"
                     /translation="MMSALVSVLVLVAVVPATAQAGPPTTTTVSATPSAVPPSTPDEP
                     SPADSADVVSASRDQVRARVAALDEQTRAAEAAAAEAQGRVDAARARAADAQAKADQA
                     AAAAAEATEQVRSYAVEAFVRPPAQDSLSTLAIEESRDAAYATDMLHIVAERRRRVVD
                     TMTAARVVAEQDRAAASTAADAASAEAEQARTQVESLRSARAQQSRLASQLDDRLDRM
                     LAEAAALREVDSRAAQEITAQ"
     gene            complement(914..1654)
                     /locus_tag="PLZ72_15505"
     CDS             complement(914..1654)
                     /locus_tag="PLZ72_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNA37755.1"
                     /translation="MPIHGSLFQEYAETTGQDPFTLQNKKMLKIQMQYGPVNTKLGSM
                     VAYQGDVRFQNTGSGGLNKMLKSKLTGEGVNMMVAEGTGELFVADLASEIQLFYLEND
                     MVSVNGNNVLAFSSSIEWDIHRVNAGRASMMAGGMFNVSLRGTGYVAVTTKGDPVCLD
                     VAGAPTYADAEAVVLWTSGVSMDVRVDTGGLKSMLRGGTGELIQMAFGGQGYVMIQPS
                     ESVVQGGAQQAQQQQSGAGGMLGGLLGG"
     gene            complement(1735..>3514)
                     /locus_tag="PLZ72_15510"
     CDS             complement(1735..>3514)
                     /locus_tag="PLZ72_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNA37756.1"
                     /translation="AGARDLSAPEPVLALARRMADLPRHLGIHSGGMVICDRPVIEVC
                     PTEWARMENRSVLQWDKDDCAAAGLVKFDLLGLGMLSALHGCLDLLRDHLGIEVDLAA
                     LPQEDAVYDMLCRADSVGVFQVESRAQMATLPRLRPRCFYDLVVEVALIRPGPIQGGS
                     VHPYIRRRKGEEPVTFLHPLLEPSLTKTLGVPLFQEQLMQMAIDVAGFSAGEADQLRQ
                     AMASKRSERRMAELRQRFFDGLAANGIVGDTATAVWEKLAAFANYGFPESHSVSFAYL
                     VYASSWLKHHHPAAFCAALLNAQPMGFYSPQSLTQDAVRHGVRVRTPDLNASGAAATL
                     ETSTEPGPGRDDADPATWGRGGPAVRLGVGSVRGIGTDLAEEIATGRPYADIADLARR
                     VRLSTAQMESLATAGAFGCFTDVDGSPLPRRQAIWSAGAAALAGADRLPGVVVGLDAP
                     RLPGMDEWEESVADLHSTGVAPDGHPTRFLRDDLDARGVSTATALRDVPDGATVRVAG
                     VVTHRQRPATASGVTFLNLEDETGLVNVVCSIGCWTRFRAVLRSAAALEVRGKLEHSS
                     DGVLNVVAHHVSPLRVAVGARSRDFR"
BASE COUNT          486 a         1263 c         1290 g          475 t
ORIGIN      
        1 cctgggcggt gatctcctgg gcggcccggg agtcgacctc gcgcagcgcc gcagcttcgg
       61 ccagcatccg gtccaagcgg tcgtcgagct gcgacgccag ccgtgactgc tgcgcccgcg
      121 ccgaccgcag cgactcgacc tgggtccggg cctgctccgc ctccgcggac gccgcgtcgg
      181 ctgcggtcga ggcggcggcc cggtcctgtt cggcgacgac cctggccgcg gtcatcgtgt
      241 cgacgacccg acggcgacgc tccgcgacga tgtgcagcat gtcggtggcg taggcggcgt
      301 cgcgggactc ctcgatggcc agcgtggaca gcgagtcctg ggccgggggg cgcacgaacg
      361 cctcgacggc ataggagcgg acctgctcgg tcgcctcggc tgccgccgcg gccgcctggt
      421 cggccttcgc ctgggcgtcc gcagcccgtg cacgggccgc gtcgactcga ccctgggcct
      481 cggccgcggc cgcctcggcc gcccgggtct gctcgtcgag cgccgcgacc cgggcccgga
      541 cctggtcccg gctggcgctg accacgtcag cggagtcagc cggggagggc tcgtcgggcg
      601 tgctcggcgg gaccgccgac ggagtcgcgg agacggtcgt cgtggtgggg ggaccggcct
      661 gcgcggtggc cggcaccacg gcgaccagga ccaggaccga cacgagagcc gacatcaccg
      721 ccgcaacccg ggacggccca gcagctgcgc ggatcggggc ggggtcggcg ttcagcacag
      781 tggatccaac gtggtgacgc gggcgcacgt tgacacgcat cggcaggtcg gggcgcgcga
      841 tgaagaaccc gggtgggaga tgtcccaccc gtcggtcccg ccgacccgtc gtgtgcccac
      901 cggatgcgcc gggtcagccc ccgaggagcc ccccgagcat cccgccggct ccggactgct
      961 gctgctgggc ctgctgggcg ccgccctgca ccaccgactc cgacggctgg atcatcacgt
     1021 agccctggcc gccgaacgcc atctggatga gctctccggt gccgccccgc agcatcgact
     1081 tgagtccacc ggtgtcgacc cggacgtcca tgctcacccc cgaggtccac agcacgaccg
     1141 cttcggcgtc cgcgtaggtc ggggcgccgg cgacgtcgag gcagaccgga tcgcccttgg
     1201 tggtgaccgc cacgtagccg gtgccgcgga ggctcacgtt gaacatgccg ccggccatca
     1261 tcgatgcccg accggcgttg acccggtgga tgtcccactc gatcgacgac gagaacgcca
     1321 gcacgttgtt gccgttcacc gagaccatgt cgttctccag gtagaacagc tggatctccg
     1381 aggccaggtc ggcgacgaac agctcgccgg tgccctcggc gaccatcatg ttgacgccct
     1441 cgccggtcag cttggacttg agcatcttgt tgagtccgcc cgagccggtg ttctggaacc
     1501 gcacgtcgcc ctggtaggcg accatcgagc cgagcttggt gttcaccggc ccgtactgca
     1561 tctggatctt cagcatcttc ttgttctgga gcgtgaacgg atcctggccg gtggtctccg
     1621 cgtactcctg gaacaacgaa ccgtggatgg gcatgggggt ctcctgtgga cgctcggatg
     1681 gggcggtcct cttcctggcg gcaacggtag cgtcgggccc cgccgatgcg gcggctatcg
     1741 gaagtcccgc gaccgggcgc cgaccgccac ccgcaacggg ctgacgtggt gggccacgac
     1801 gttgagcacc ccgtcggagc tgtgctcgag cttcccgcgg acctccagcg ccgccgccga
     1861 gcgcaggacg gcccggaagc gggtccagca cccgatcgag cacaccacgt tgacgaggcc
     1921 ggtctcgtcc tcgaggttca ggaacgtgac ccccgaggcg gtggccggac gctggcggtg
     1981 ggtgaccacc ccggcgacgc ggaccgtggc gccgtcgggc acgtcgcgaa gagcggtggc
     2041 ggtgctcacc ccccgggcgt cgaggtcgtc acgcaggaac cgggtggggt ggccgtcggg
     2101 ggcgacgccg gtggagtgca gatccgcgac cgactcctcc cactcgtcca tgcccggcag
     2161 ccggggcgcg tccaggccga ccaccacccc cggcaaccgg tcggccccgg cgagcgcggc
     2221 ggcacccgcg gaccagatgg cctgacgccg gggcagcggg gagccgtcga cgtcggtgaa
     2281 gcagccgaac gccccggcgg tggcgagcga ctccatctgg gcggtgctga gccggacccg
     2341 gcgggccaga tccgcgatgt cggcgtaggg gcggccggtt gcgatctcct cggccaggtc
     2401 ggttccgatg cctcgcaccg agccgacgcc caaccgcacc gccggacctc cccgacccca
     2461 cgtggccgga tcggcgtcgt cgcgccccgg acccggttcc gtggacgtct ccagggtggc
     2521 cgccgcgccc gacgcgttga ggtcgggggt gcggacccgg acgccgtgac ggaccgcgtc
     2581 ctgggtgagc gactgcggcg agtagaagcc catcggctgg gcgttgagca gcgcggcgca
     2641 gaacgcggcc gggtggtggt gcttgagcca cgacgacgcg tagaccaggt aggcgaagct
     2701 caccgagtgc gactcgggga acccgtagtt ggcgaacgcg gcgagcttct cccacaccgc
     2761 ggtcgccgtg tcgcccacga tgccgttggc ggccagaccg tcgaagaacc gctgacgcag
     2821 ctcggccatg cgccgctcgg agcgcttgga ggccatcgcc tggcgcagct ggtcggcctc
     2881 cccggcgctg aagccggcca cgtcgatcgc catctgcatg agctgctcct ggaacagcgg
     2941 gacgcccagg gtcttggtga gcgacggctc gagcagcggg tgcaggaacg tgacgggctc
     3001 ctcgcccttg cgacgccgga tgtaggggtg caccgaaccg ccctggatgg gcccgggccg
     3061 gatgagcgcc acctcgacca ccaggtcgta gaagcaccgg ggccgcaggc ggggcagcgt
     3121 cgccatctgg gcccgggact ccacctggaa cacgccgacc gaatcggccc ggcacaacat
     3181 gtcgtagacg gcgtcctcct ggggcagggc ggccaggtcg acctcgatgc ccaggtggtc
     3241 gcgcaggagg tccaggcagc cgtgcagcgc cgagagcatg cccagcccga gcaggtcgaa
     3301 cttgaccagg ccggccgccg cgcagtcgtc cttgtcccac tggagcaccg agcggttctc
     3361 catccgggcc cactcggtgg ggcacacctc gatgaccggc cggtcgcaga tgaccatgcc
     3421 gccggagtgg atgcccaggt gccggggcag gtccgccatg cggcgggcca gggccagcac
     3481 cggttcgggg gcgctcaggt cccgggcccc ggcg
//
LOCUS       DAOQOD010000259         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_13108_length_3487_cov_10.791375, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000259 DAOQOD010000000
VERSION     DAOQOD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13108_length_3487_cov_10.791375"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1372)
                     /locus_tag="PLZ72_15515"
     CDS             complement(71..1372)
                     /locus_tag="PLZ72_15515"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNA37757.1"
                     /translation="MSTNDELYQRGLRVIPGGVNSPVRAFRSVGGTPYFVASGSGARV
                     IDVEGREFIDYVQSYGASILGHAHPVVVDAVRDAAGRGSTFGAPTEGEVRLAEEIVSR
                     IPGMEQVRLVSSGTEATMSAIRLARGVTGRDRVVKFEGNYHGHSDALLAAAGSGVAEA
                     VLGTGARPDSGGVTAGAVADTVVHPYNVVPTLDDTVAVVIVEPVAANMGLVPPVDGFL
                     TGLRSECDRVGALLLFDEVITGFRLGVGGATGWSGVTPDLWCFGKVIGGGLPVGAFGA
                     SAAVMSSIAPLGPVYQAGTLSGNPLATAAGLAVLSQLDASAYERLVATATALADGLSA
                     AFADAGVAAVVPRVGPLVGLFFLPDTPDASDPRGGVPVDFATVAASVDLGRYPAWFHG
                     MLDHGIALAPGPYEVMFPSLAHTDADVEATLAAARAVAPTL"
     gene            complement(1425..2405)
                     /gene="hemB"
                     /locus_tag="PLZ72_15520"
     CDS             complement(1425..2405)
                     /gene="hemB"
                     /locus_tag="PLZ72_15520"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNA37758.1"
                     /translation="MSFPQTRLRRLRRTPAMRRLVAETRLGVDDLVAPLFVREGIDEP
                     VPIPSLPGVVQHTRSSLRAEVAELADLGVNAVILFGVPEHKDAEGSGASDPDGIVQVA
                     LRDLRDDLGDRVVLMADLCIDEYTDHGHCGLLAPDGTVDNDATLEVYGEAAIAQADAG
                     ADVCAPSGMMDGQVLAIRDALDDSDHEQVSILAYSAKYASALYGPFRDAVDVHIADGG
                     DRRAYQQDWANAREGLSELREDLMEGADMVMVKPALTYLDVLTRARAEIDVPIAAYHV
                     SGEYAMVHAAAERGWLDLDAVALEQITAVKRAGADMVLTYFARRLAELLQ"
     gene            complement(2499..3179)
                     /locus_tag="PLZ72_15525"
     CDS             complement(2499..3179)
                     /locus_tag="PLZ72_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNA37759.1"
                     /translation="MSAAAPALAAVVFDFDGLVLDTEWCEYVTAAEQFRAHGTELSLD
                     LWKTFIGTVDHPHWTDILEEQIGRPVDRGLIVPSRLAAKDVCNADLAPLPGVVPLVDS
                     LRDLGVPIAVASSSSVDHVDGHLDTHGLLDRFPVRATGDEVPLTKPDPAVYLLACERL
                     GVDPTAAVAIEDSVNGVTAAKAAGMAAVAVPGTLTLGMDFAAADLVVGSCVELTPARL
                     AALVADRN"
     gene            complement(3176..>3487)
                     /locus_tag="PLZ72_15530"
     CDS             complement(3176..>3487)
                     /locus_tag="PLZ72_15530"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNA37760.1"
                     /translation="RTVGVSYDDDTRARVRAADAVTFTSSSTAEHFVAALGGPDAAAA
                     GAPPVVACIGPVTAATARELGLEVTAEAAVHSIDGLVDAVVDAVSGVGVGVVPGGSDA
                     R"
BASE COUNT          491 a         1209 c         1365 g          422 t
ORIGIN      
        1 agtgcgagat ccaggcgttc gcctggatct cgcactacag aacgggagtg ggggggctag
       61 ggggtggggg ctagagggtg ggggcgacgg cgcgggcggc ggcgagggtg gcctcgacgt
      121 cggcgtcggt gtgcgcgagc gacgggaaca tgacctcgta cggcccggga gccagggcga
      181 tgccgtggtc caacatcccg tggaaccacg ccgggtagcg gcccaggtcg accgacgccg
      241 ccacggtggc gaagtcgacc ggtaccccgc cgcgggggtc ggaggcgtcg ggggtgtcgg
      301 gcaggaagaa caggccgacc agcgggccga cgcgggggac gaccgcggcc acgccggcgt
      361 cggcgaacgc ggcgctcagc ccgtcggcca gcgcggtggc ggtggcgacg agccgctcgt
      421 acgcggatgc gtcgagctgg gacagcacgg ccaggccggc ggcggtggcc agcgggttcc
      481 ccgacagcgt gccggcctgg tacaccggcc cgagcggggc gatcgacgac atgaccgcgg
      541 cggacgcccc gaaggcgccg accggcaggc cgcccccgat caccttgccg aagcaccaca
      601 ggtccggcgt cacccccgac cacccggtgg cgccaccgac gccgaggcgg aacccggtga
      661 tgacttcgtc gaacagcagt agcgcgccga cgcggtcgca ctcggagcgc aggccggtga
      721 ggaacccgtc gaccgggggg accaggccca tgttggcggc gaccggctcg acgatcacga
      781 ccgcgaccgt gtcgtccagg gtcggcacca cgttgtaggg gtgcacgacc gtgtcggcga
      841 ccgctccggc ggtcacgccg ccggagtcgg gtcgggcgcc ggtgccgagc acggcctcgg
      901 ccacaccgga gccggccgcg gcgagcagcg cgtcggagtg gccgtggtag ttgccctcga
      961 acttcacgac gcggtcccgg ccggtgacgc cgcgggccag gcggatggcc gacatggtcg
     1021 cctcggtgcc cgagctgacc aggcgcacct gctccatgcc ggggatgcgg gagacgatct
     1081 cctcggccaa gcggacctcg ccctcggtgg gtgccccgaa ggtggagccg cggccggccg
     1141 cgtcgcgcac ggcgtcgacg acgacggggt gggcgtggcc gaggatcgac gcgccgtagc
     1201 tctggacgta gtcgatgaac tcgcggccct cgacgtcgat gacccgggcc ccggagcccg
     1261 acgcgacgaa gtacggggtg ccgccgacgg agcggaaggc ccggaccggg gagttgaccc
     1321 cgccggggat gacccgcagg ccccgctggt acaactcgtc gttggtgctc acgcgctcgc
     1381 tcctcggtcg gtggccgaac ccgcgacggg cccgtcggtg gcggtcactg gagcagctcg
     1441 gccaggcggc gggcgaagta ggtgaggacc atgtcggccc cggcccgctt gaccgcggtg
     1501 atctgctcga gcgcgaccgc gtcgagatcc agccagcccc gctcggctgc ggcgtgcacc
     1561 atggcgtact cgccggacac gtggtacgcc gcgatcggca cgtcgatctc ggcccgggcc
     1621 cgggtgagga cgtcgaggta ggtcaaggcc ggcttgacca tcaccatgtc ggcgccctcc
     1681 atcaggtcct cgcggagctc cgacagaccc tctcgggcgt tggcccagtc ctgctggtag
     1741 gcccggcggt cgccgccgtc ggcgatgtgc acgtcgaccg cgtcgcggaa cgggccgtag
     1801 agagccgagg cgtacttggc cgagtaggcc aggatcgaca cctgctcgtg gtcgctgtcg
     1861 tcgagtgcgt cgcggatggc gaggacctgg ccgtccatca tccccgacgg ggcgcacacg
     1921 tcggccccgg cgtcggcctg agcgatcgcg gcctcgccgt agacctcgag ggtggcgtcg
     1981 ttgtcgacgg tgccgtcggg ggcgagcaga ccgcagtggc cgtggtcggt gtactcgtcg
     2041 atgcagaggt cggccatgag cacgacccgg tcgccgaggt cgtcgcgcag gtcccgcagc
     2101 gcgacctgca cgatgccgtc gggatcggat gcgccggacc cctcggcgtc cttgtgctcg
     2161 ggcacgccga agaggatcac ggcgttcacg ccgaggtcgg cgagctcggc cacctcggcg
     2221 cgcagcgacg accgcgtgtg ctgcaccacc ccgggcagcg acgggatcgg caccggctcg
     2281 tcgatgccct cacgcacgaa caggggggcg accaggtcgt cgacacccag tcgggtctcg
     2341 gcgaccagcc gccgcatggc cggggtgcgg cgcagtcgac gcaggcgggt ctgggggaag
     2401 ctcacggccc cgaatcctac ggtcgggtcc acgaccggct gcagttgggg ccgccgttgc
     2461 ggccgggcgt gcggccagga ccgtgggtcg ggttcggctc agttccgatc ggcgaccagg
     2521 gccgcgagcc gggcgggggt gagctcgacg caggatccga cgaccaggtc ggcggcggcg
     2581 aagtccatgc cgagcgtcaa cgtgccgggg acggccaccg cggccatccc ggccgccttg
     2641 gcggcggtca ccccgttgac cgagtcctcg atcgccaccg ccgcggtcgg gtccacgccg
     2701 agccgttcgc acgccaggag gtagaccgcg gggtcgggct tggtgagcgg aacctcgtcg
     2761 ccggtggcgc gcaccgggaa ccggtcgagc aggccgtggg tgtcgaggtg gccgtcgacg
     2821 tgatcgaccg acgacgacga cgccaccgcg atcggcaccc cgaggtcgcg caacgagtcg
     2881 acgagcggca cgacgccggg cagcggggcc agatcggcgt tgcagacgtc cttggcggcg
     2941 aggcgggacg ggacgatcag cccacggtcg accggccggc cgatctgctc ctcgaggatg
     3001 tcggtccagt gcgggtggtc gacggttccg atgaaggtct tccacagatc caggctcagc
     3061 tcggtgccgt gcgcccggaa ctgctcggcg gcggtgacgt actcgcacca ctcggtgtcg
     3121 agcaccagcc cgtcgaagtc gaacacgacg gcggccagag cgggggccgc ggcgctcaac
     3181 gggcgtccga cccgccgggc acgacgccga caccgacgcc ggacacggcg tccaccacgg
     3241 cgtcgacgag gccgtcgatc gagtgcaccg cagcctcggc ggtgacctcc aggcccagct
     3301 cgcgggcggt cgccgcggtg accggtccga tgcaggcgac cacgggcggg gctccggccg
     3361 cggcggcgtc gggtccgccg agggcggcga cgaagtgctc ggcggtcgag ctcgacgtga
     3421 acgtgaccgc gtccgcggca cgcacccggg cccgggtgtc gtcgtcgtag gagaccccga
     3481 cggtccg
//
LOCUS       DAOQOD010000260         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_13268_length_3461_cov_9.320317, whole genome shotgun sequence.
ACCESSION   DAOQOD010000260 DAOQOD010000000
VERSION     DAOQOD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_3461_cov_9.320317"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..341)
                     /locus_tag="PLZ72_15535"
     CDS             complement(81..341)
                     /locus_tag="PLZ72_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37761.1"
                     /translation="MTAWRDVGAAAPEFAARVRGLFEARTHKTIAAMCADGAPRILGT
                     ECEFVDGELQFGLMIGAREGADLRRTARQRPESCCVLCGNLK"
     gene            complement(338..1447)
                     /locus_tag="PLZ72_15540"
     CDS             complement(338..1447)
                     /locus_tag="PLZ72_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNA37762.1"
                     /translation="MLRSGQQRRQPDHGVEAVTEPHESELEPLREQLLELDPAAASLV
                     GNRDLFGQLATAAMLDPLREAVARWEPDLIVRDPCEYASAVVAAETSLPVAQAAISLA
                     AVEWGSIAAAAPALEGWRVGLSDIVRSTPYVTRFPASLDPSPFPTTIRFREPWVPAEP
                     LPDWWPGDDRPLVYATFGTVLGHMSTAVRSMAVLLDAVAGLDARVLLTTGRGVDPVAL
                     GPAPANVRVEQWVDQADALEAASVVVCHGGSGTTFGALARGVPVVVVPMFADQPVNAV
                     AVERAGAGTVVWPADRRSLGDGDAERIERAVRVIVDRSRSRPDLGGPRHAAAAIGAEM
                     AALRLLLDVFPGDRGAVSPWVGDVDQLHGDPGTSR"
     gene            1409..1915
                     /locus_tag="PLZ72_15545"
     CDS             1409..1915
                     /locus_tag="PLZ72_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNA37763.1"
                     /translation="MVRLATLLTGSQHEAEEIVMDAFARMAARPAGLRDLDEPAAYLR
                     TSVVNGAHSRHRRLRTLRSAPAHRPSPHTDTQFDDLWARLATLTDDQRRCVVLRFYED
                     LPLAQIAEVLDMPVGTVKSHLHRALGRLRPLLDHDGHRPAPPGMRSGPAEEPGVHGDD
                     DDHIGGDR"
     gene            1912..3201
                     /locus_tag="PLZ72_15550"
     CDS             1912..3201
                     /locus_tag="PLZ72_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37764.1"
                     /translation="MTDHLDPSELTDPHDLAITSADEDRLRALLHAAADDLEIDPEHD
                     AHGAITRPPTRSRRTPRTVAAVAAAAIAVVTVGGVIWAAGGDDPTVVDPAGPGHSTPT
                     VATTDLGPDTWRDGAGIWRLPDPASGLVIEQALMSRTGEPPWLVALDPGGDSSRFVAV
                     SLLFVEQYQDPDPETLTRYSTVDDVDVSMNASAGGEAAGLDWTAVRAREGRGGLAIIS
                     HGIEADEIHAYASDLAALIAREPGTISAEVAAQALQAATLPSGLTPSWAEADAVGILD
                     GRRSTTGLTLGVTRSLGPTILVVIDPPPAGAVTAHWIASVLTSLERKATTSDLLEPTS
                     EIWSPGETANRLQGFTGDGTSITVSGSASREELTAVLRSLRATDEAAARQRLRDDGIA
                     LGTVDPAVPTTTMVPDAIASDGLVPGGHTSTTAPGRP"
BASE COUNT          554 a         1314 c         1159 g          434 t
ORIGIN      
        1 ccggtcagat ctccggtggg ccaatgccgg cggatcatgc gcgccatcgc gtctccctga
       61 acctccaacc ggccgtcacg ttatttcagg ttaccgcata acacgcaaca tgactcgggt
      121 cgctgtcggg ctgtgcggcg caggtcggct ccctcgcgcg ccccgatcat caacccgaac
      181 tgcagctcgc cgtcgacgaa ctcgcactcg gtgcccaaga tccgcggagc gccgtcggcg
      241 cacatcgcgg cgatcgtctt gtgagtccgg gcctcgaaca acccccggac ccgggcggcg
      301 aactcggggg ccgcggcccc gacgtcacgc cacgccgtca tcggctggtc ccgggatctc
      361 cgtgcaactg gtccacgtcg ccaacccaag gcgacacggc gccgcggtca ccggggaaca
      421 cgtcgagcag cagccgcagc gcggccatct ccgccccgat cgccgcggcg gcatgccggg
      481 gaccgccgag gtcgggccgg gaccgggatc tgtcgacgat cacccgcacc gcccgttcga
      541 tccgctcggc gtcgccgtca ccgagactgc gacggtcggc gggccacacc accgtccccg
      601 ccccagcccg ctccaccgcc acggcattga cgggctgatc ggcgaacatc gggaccacaa
      661 ccaccggcac ccctcgggcc agggcgccga aggtcgttcc cgaaccgccg tggcacacca
      721 cgaccgacgc cgcctccagc gcgtcggcct gatcgaccca ctgctcgacc cggacgttgg
      781 cgggggccgg tcccagcgcg acgggatcca cgccacggcc ggtggtcagc aggacgcggg
      841 catcgaggcc cgctacggcg tcgaggagca ctgccatcga gcggacagcc gtcgacatgt
      901 gcccgaggac ggtgccgaac gtggcgtaca ccagcggacg gtcatcgccc ggccaccagt
      961 ccggcagtgg ctcggccgga acccacggct cccggaacct gatcgtggtc gggaacggcg
     1021 acggatccag ggacgcggga aagcgggtca cgtagggggt ggagcggacg atgtcggaca
     1081 gcccgactcg ccacccctcg agagctgggg ccgctgcggc gatcgatccc cactcgacag
     1141 cggcgagcga gatggcggct tgcgcgaccg gaagggaggt ctccgccgcc accaccgctg
     1201 aggcgtactc gcacggatca cggacgatga ggtccggctc ccaccgagcg accgcctccc
     1261 ggagcgggtc gagcatcgcc gctgtggcga gctgtccgaa gaggtcgcgg ttgccgacga
     1321 gcgacgcggc ggcggggtcg agctcgagca gctgctcgcg gagcggctcc agttcggact
     1381 cgtggggctc cgtgaccgct tcgaccccat ggtccggctg gcgacgctgc tgaccggatc
     1441 gcagcacgag gccgaggaga tcgtgatgga cgccttcgcc cggatggccg cccgccccgc
     1501 cgggctccgc gacctcgacg agccggcggc ctatctccgc acgagcgtcg tcaacggcgc
     1561 ccactcccga caccgccgac tccggaccct gcgatcggca ccagcgcatc gaccctcgcc
     1621 gcacaccgac acgcagttcg acgatctgtg ggcccggctc gccacgctca ccgacgacca
     1681 acgtcgctgc gtcgtccttc gcttctacga ggacctgccc ctggctcaga tcgcggaggt
     1741 gctcgacatg ccagtcggca cggtcaagtc gcacctgcac cgagcgctcg ggcggttgcg
     1801 cccgctcctc gatcacgacg gccacaggcc cgccccaccg gggatgagat ccgggccggc
     1861 cgaggagccc ggcgtccacg gtgacgacga cgaccacatc ggaggcgacc gatgacagac
     1921 cacctagacc ccagcgagct cacggacccc cacgacctcg ccatcacatc ggccgatgag
     1981 gaccgcctga gagcgctgct ccacgcggcg gccgacgacc tcgagatcga cccggaacat
     2041 gacgctcacg gagcgatcac ccgaccaccg acgcggtccc gtcgcacacc ccggacagtc
     2101 gccgcagtcg ccgccgcggc gatcgcggtc gtgacggtcg gcggggtgat ctgggccgcc
     2161 ggcggggacg accccaccgt cgtggacccg gccggtccgg gccactccac ccctacggtg
     2221 gcaaccaccg acctggggcc cgacacatgg cgcgatgggg cgggcatctg gcgactcccc
     2281 gaccccgcgt caggcctcgt catcgaacag gcgttgatga gccggaccgg ggagccgccg
     2341 tggctggtcg cgctcgaccc gggcggtgac tcctcacggt tcgtcgccgt ctccctcctg
     2401 ttcgtcgagc agtaccagga tcccgatcca gagacgctga cgcggtactc gacggtcgac
     2461 gacgtcgacg tgtccatgaa cgcgtccgcc gggggcgagg cggccgggct cgactggacc
     2521 gcggtccgag cccgggaggg tcgcggtggc ctcgccatca tcagccacgg gatcgaggcc
     2581 gacgagatcc acgcctacgc ctccgacctg gcggcgctga tcgcccggga gcccgggacc
     2641 ataagcgccg aggttgcggc ccaggccctg caagccgcga cgctgccgtc gggcctgacg
     2701 ccttcgtggg ccgaggccga tgcagtcggg atcctcgacg gccgacgatc gaccaccggc
     2761 ctgacgctgg gtgtgacgcg gtccctgggt cccacgatcc tcgtggtcat cgacccgcct
     2821 ccggcgggtg cggtcacagc ccactggatc gcctcggtgc tgacctcgct cgagcggaag
     2881 gctacgacgt cggacctgct cgagccgacc tcggagatct ggagccccgg cgagaccgcc
     2941 aaccggctgc agggattcac cggggacggc acgtcgatca cggtgtccgg ctccgcctca
     3001 cgggaggagt tgaccgcggt gctccgcagc ctccgggcca ccgacgaggc ggccgctcgt
     3061 cagcgtctgc gcgacgacgg gatcgccctc ggcaccgtgg accccgctgt tccgaccacg
     3121 acgatggtcc ccgacgcgat cgcttccgac ggtttggtcc ccggtggcca cacgtcgacc
     3181 accgcgcccg gccggccctg aggtcgatcc tgtcgaactt gtagcccaca cgccaccaca
     3241 gcgaccggga gctacaggtt cgacacggac cagcgaactt gcagcccaca cgccaccaca
     3301 gcgaccggga gctacaagtt cgacacggac cagcgaactt gcagcccaca cgccaccaca
     3361 gcgaccggga gctacaagct cgccgggtca ggacgtgcca aacggggctg gcgactactc
     3421 gatgcgcagg ccggagatgg cccggccgat cacgagctgc t
//
LOCUS       DAOQOD010000261         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14061_length_3345_cov_12.413374, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000261 DAOQOD010000000
VERSION     DAOQOD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14061_length_3345_cov_12.413374"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLZ72_15555"
     CDS             <1..600
                     /locus_tag="PLZ72_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNA37765.1"
                     /translation="PPADDPDGSPRLHVDDRTAVLDDDLRPVAPGSGTVGRLARGGRV
                     PLRYLGDPEKSAATFVEVGGVRWALPGDLATLEDDGTIVILGRSSQCINTGGEKVYPE
                     EVEAALKAHPDVVDAVVVGVADERWGQSVSAVVQPRPGTTPTLDELRDAARDQLAGYK
                     LPRRLVLVDEVLRSPAGKPDHRWAQSVAAAAAPPGEAGP"
     gene            606..1973
                     /locus_tag="PLZ72_15560"
     CDS             606..1973
                     /locus_tag="PLZ72_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNA37766.1"
                     /translation="MSGLDARFFYSETPTAHMHTMKVVVVDPSGRTVPLADEGIPTLV
                     EARLARLPMLRRRIIPAPHGLGNPSVVDDPEFDLSRHLRTAHVDPPGGQRELDRVIAD
                     IAGVALPRDRPLWEMTIVDGLEGGRVAFVMKLHHAIADGVASVAMLENAFVADSAHAV
                     EEPFRPEPLPSSRQLYQAAATGAARAVRSMPRAAVRTVAGARQARAVKQSVTALLPGP
                     FTGPRTPFNVALSSDRTFSGVGIPLEVLLAAKKTAGASLNDAFLALCAGAIRRYLGRL
                     GALPSASLITSVPMATRTDRHRLGGNHVDNLFLPLRTDVADARSRIEAIHESSVAARR
                     VREEFGPDLFEWRSGLVPAGMHGLVPRVWGRTRLADHLRPPLNLIASCVKGPRERLEI
                     DGGVVTSLFSSGPILEGIGVNITAWSYVDTMYISVLGCSASLPDPWRLTEDLVAESDS
                     WADWI"
     gene            2020..3342
                     /locus_tag="PLZ72_15565"
     CDS             2020..3342
                     /locus_tag="PLZ72_15565"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNA37767.1"
                     /translation="MTPVASADVPDTDDPEPTERSGTDERSCSDPGPWWHPGANVRRV
                     TSATKQVEKRLARQPMARMTLAVPRTARFVVGEMLGEDVPGVPSARLTPALVGHVLMD
                     ESIMAIAIGPNRFPRRADYPRVADELSRAHDLFSERGWLDDPASYHQDPPPLEEPAVT
                     KGWALGHAYERLWWPSGYTPHLGVPGTDRWLAFESNRTASAWVLRHRDGPRPWVVCVH
                     GFGTGSVFMDLFAFRAAHLHEQLGVNVAAIVLPVHGARRPSRLSGEEFLGFEFMNSVH
                     GLTQAVWDVRRLLSWVRRQDPTALGVFGVSLGGMVTGLVSAFEPDLDMALTGIPIVDF
                     PGLIEHHAPRHLQMRSIEHNILNGTAQDVHKVVSPLAMAPVTRPGSRAIFAGLGDRLA
                     TTDQARRLWEHWDEPETCWFAGNHVGYLWSDTVWQFVDHVFQSRGLTA"
BASE COUNT          437 a         1254 c         1182 g          472 t
ORIGIN      
        1 ccgcccgccg atgaccccga cggctcgccg cgcctgcacg tcgacgaccg caccgctgtg
       61 ctcgacgacg acctgcgacc ggtcgccccc ggatcgggga ccgtcggccg cctggcccgc
      121 ggcggccggg tgccgctgcg ctacctgggc gacccggaga agagcgcggc gaccttcgtc
      181 gaggtgggcg gggtccgctg ggcgctgccg ggcgacctgg cgactctcga ggacgacggc
      241 acgatcgtga tcctgggtcg gtcgtcgcag tgcatcaaca ccggcggcga gaaggtctac
      301 cccgaagagg tcgaagcggc cctgaaggcc cacccggacg tggtcgacgc cgtcgtggtg
      361 ggagtcgccg acgagcgatg gggccagtcg gtgagcgcgg tggtgcaacc gcgtcccggc
      421 acgacgccga ccctcgacga gctgcgcgac gcggcccggg accagctggc cggctacaag
      481 ctgccccgac ggctggtgct ggtcgacgag gtgctccggt cgccggccgg caagcccgac
      541 caccgctggg cccagtcggt ggccgccgcg gccgctcccc ccggggaggc gggtccgtga
      601 aggtcatgtc cggcctcgac gcccggttct tctactcgga gaccccgacg gcgcacatgc
      661 acaccatgaa ggtggtggtc gtcgacccgt cgggacggac ggtgccgctg gccgacgagg
      721 ggatcccgac gctcgtcgaa gcccggctgg cccgactgcc gatgctgcgg cgacggatca
      781 tccccgcccc gcacggtctg gggaacccgt cggtcgtcga cgaccccgag ttcgacctct
      841 cgcgtcacct tcgcaccgcc cacgtcgacc cgccgggcgg ccagcgcgag ctcgatcggg
      901 tcatcgccga catcgccggg gtggctctgc cgagggaccg cccgctgtgg gagatgacca
      961 tcgtcgacgg cctcgagggc ggccgggtgg cgttcgtgat gaagctgcac cacgccatcg
     1021 ccgatggcgt ggcgtcggtg gccatgctcg agaacgcttt cgtcgccgac tccgcccatg
     1081 ccgtcgagga gcccttccgg ccggagccgc tgcccagctc ccgacagctc taccaggcgg
     1141 ctgcgaccgg agccgcccgg gcggtgcgct ccatgccccg ggccgcggtc cggaccgtgg
     1201 ccggcgcccg ccaggcccgg gcggtcaagc agtcggtgac ggccctgctt cccgggccgt
     1261 tcaccggtcc gcgcaccccg ttcaacgtgg cgctcagctc ggaccgcacg ttctcgggag
     1321 tcgggatccc gctcgaggtg ctgctcgccg ccaagaagac cgccggggcg tcactcaacg
     1381 acgcgttcct ggcgttgtgc gccggagcaa tccgccggta cctcggacgg ctgggagcgt
     1441 tgccgtcggc cagcctgatc acctcggtgc cgatggcgac gcgcaccgac cgccaccgcc
     1501 tcggcggcaa ccacgtcgac aacctcttcc tgccgctgcg gaccgacgtc gccgatgccc
     1561 ggtcccgcat cgaggccatc cacgagtcgt cggtggcggc ccgccgggtc cgcgaggagt
     1621 tcggccccga cctgttcgag tggcgctccg gcctggtgcc cgccgggatg cacggcctgg
     1681 tcccccgggt ctggggccgg acccggctgg cggaccacct gcgacctccg ctcaacctga
     1741 tcgcctcgtg cgtcaagggg cctcgggagc gactcgagat cgacggcgga gtggtgacgt
     1801 cgctgttctc gtcggggccg atcctcgagg ggatcggggt caacatcacc gcctggagct
     1861 acgtcgacac catgtacatc tcggtgctcg gctgctcggc gtcgctgccg gacccgtggc
     1921 ggctgaccga ggacctcgtc gccgaatccg actcgtgggc tgactggatc tgagccggac
     1981 cggtacggtg ggaaccagcg atctcggggg aggtcccgca tgacgcccgt cgcatccgca
     2041 gacgtacccg acaccgacga cccggagccg accgaacggt ccgggaccga cgagaggtcc
     2101 tgttcggacc ccggcccgtg gtggcacccg ggcgccaacg tccgccgggt cacctcggcg
     2161 accaagcagg tggagaagcg cctggcccgc cagccgatgg cccgcatgac cctggcggtg
     2221 ccccgcaccg cccgcttcgt cgtgggcgag atgctgggcg aggacgtccc cggggtcccc
     2281 tcggcgcgac tcaccccggc gctggtcggt cacgtcctga tggacgagtc gatcatggcg
     2341 atcgcgattg gccccaaccg cttcccacgc cgcgccgact acccgcgggt ggccgacgag
     2401 ctgagccggg cccacgacct gttctccgag cggggctggt tggacgaccc ggcgtcgtac
     2461 caccaggatc cgcccccgct cgaggagccc gccgtcacca agggctgggc cctcggtcac
     2521 gcctacgaac ggctgtggtg gccgagcggc tacacccccc acctcggcgt gcccggcacc
     2581 gaccgctggc tggcgttcga gtccaaccgc accgcctccg cgtgggtgct gcgccaccgc
     2641 gacggtcccc gcccgtgggt ggtgtgcgtg cacgggttcg gcaccggctc ggtgttcatg
     2701 gacctgttcg ccttccgggc cgcgcacctg cacgagcagc tcggcgtcaa cgtcgccgcc
     2761 atcgtgctgc cggtgcacgg cgcccgccga ccgagccggc tgagcggcga ggagttcctc
     2821 ggcttcgagt tcatgaacag cgtccacgga ctcacccagg cggtgtggga cgtgcggcgt
     2881 ctgctcagct gggtccgccg gcaggacccg accgccctcg gcgtgttcgg cgtgtcgctc
     2941 ggcggcatgg tcaccgggct ggtctccgcg ttcgagcccg acctcgacat ggcgttgacc
     3001 ggcatcccga tcgtcgactt cccgggcctg atcgagcacc acgcgccgcg gcacctgcag
     3061 atgcgcagca tcgagcacaa catcctcaac gggaccgccc aggacgtgca caaggtggtg
     3121 tcaccgctgg cgatggcgcc ggtgacgcgg cccgggtccc gggcgatctt cgccgggctc
     3181 ggcgaccggc tggccaccac cgaccaggcc cgccgcctgt gggagcactg ggacgagccc
     3241 gagacgtgct ggttcgccgg caaccacgtc ggctacctgt ggtccgacac ggtgtggcag
     3301 ttcgtcgacc acgtgttcca gtcccgcggc ctcaccgcct gaccg
//
LOCUS       DAOQOD010000262         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14264_length_3319_cov_8.325980, whole genome shotgun sequence.
ACCESSION   DAOQOD010000262 DAOQOD010000000
VERSION     DAOQOD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_3319_cov_8.325980"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1607
                     /locus_tag="PLZ72_15570"
     CDS             147..1607
                     /locus_tag="PLZ72_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37768.1"
                     /translation="MAATVAAAPRSNQDPGVASPRHRTWVIPLTAAVVMVLYAASAVL
                     VARDQSGSDFHRRTAAAIAEGHLDIRPVPAELRTLPDPYDAGSNLDVRVDGDVQDLAY
                     RDGRLYSAHGLTIPLLLVPSELAFGTSPPNWVITLVAACAGVAAAAWTLAQVRRRFLC
                     DLPDWTTAAAVAAVGLCGPMWVVVSVGNGYEAAVAAGFALSMIGAALLLRSTERMSSA
                     DPDRSLERARAAAGSAVLGLAVGARPTMVVTAILLAVVAAVVVARRGSRPTASLIADL
                     LAVAGPFVVVGICIAVANAVRFGSPTEFGFGFQLSVWDMTTYPQGRLSYLAPNLLDHI
                     AAIPGHRTSFPWITLRPTIGGDRPTVHTSEPMIGLIFGAPVLVVGAVAALPSGRAPWA
                     RARGLGTAVAAAATTGALLLVLVSWPFNTSSLRYTADGAPLLLLAAAGAWLTVRSDAP
                     LASGTGAGTGGRRLDRAWLVALAVGIAVTAAVQVPT"
     gene            1635..1856
                     /locus_tag="PLZ72_15575"
     CDS             1635..1856
                     /locus_tag="PLZ72_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37769.1"
                     /translation="MSDASPLMTGPLGEPLEFTCPRCQRPAVEPFYGPCGTCRTTLRA
                     VMGGEAQEVEKVDYEPKMNVTPNAVATKD"
     gene            complement(1876..3288)
                     /locus_tag="PLZ72_15580"
     CDS             complement(1876..3288)
                     /locus_tag="PLZ72_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNA37770.1"
                     /translation="MFDLDRTLLIGASGPILSEALRRAGVVSGSTQGIESVMFKVFDL
                     IGETYPSMLVSRQGARVARGWPVDAVREAARWAAEPLADAVLPYARQLIDEHRAAGRL
                     LVMATTTPEDLIRPLADRLGFDDVVATRYGTEDGRYDGTIDGEFVWGRGKARAVSQWV
                     LDNDVDLLASYAYSDSWYDTPLLSIVGHPTVVNPDPRMLGMATVRRWPVVHFDVPAGV
                     PKFVGIEPQQVLFMLAQPQLFPWVRFDLGGLDHIPAEGPAILVGNHRSYLDPLAIGFT
                     IARRGRPVRFLGKKEVFDAPIVGEVATALGGIRVDRGTGSDAPLRAAEEALAAGQLVA
                     LMPQGTIPRGPAFFDPELKGRWGAVRLAHATGVPVIPIGLWGTEQVWPRSAKVPDLTN
                     VLHPPTVRVRVGAPVPLGGEDVAADTEAMMAAIVDLLPAAAREVRTPTPEELARTYPG
                     GVVPDGDDAEHERDRRPGTD"
BASE COUNT          429 a         1240 c         1176 g          474 t
ORIGIN      
        1 gcccgcaccc aggcctgacc cacccctccc acccccacga tcagctttgt cccgcgcagg
       61 cgcggtgatc accgcgcctg tgcgggacag agcggaggtg aggggcgcag tcctaccgtt
      121 cgggccgcgc ctgccgatgt agagccatgg ccgcaacggt cgccgccgcg ccgcgctcga
      181 accaggaccc gggggtcgcc tctccccgac accgcacctg ggtgatcccc ctgaccgcag
      241 ccgtggtcat ggtcctgtac gccgcctccg cggtcctggt ggcccgggac cagtcgggtt
      301 cggacttcca tcgccggacc gccgcggcga tcgccgaggg ccacctggac atccgtcccg
      361 tccccgccga gctgcgcacc ctcccggacc cctacgacgc cggctccaac ctcgacgtcc
      421 gagtcgacgg cgacgtccag gacctggcct accgggacgg gcggctgtac tccgcccacg
      481 gcctcaccat cccgctgctg ctggtccctt cggagctggc gttcgggacc tcaccgccca
      541 actgggtgat caccctggtc gcggcgtgtg ccggggtggc cgccgcggcg tggactctgg
      601 cccaggtacg ccgacggttc ctgtgcgacc ttcccgactg gacgacggcc gccgcggtcg
      661 ccgcggtcgg cctctgcggg ccgatgtggg tggtggtgtc ggtcggcaac ggctacgagg
      721 cggccgtggc ggccggcttc gccctcagca tgatcggcgc agccctcctc ctgcgatcga
      781 ccgagcggat gagctccgcc gatcctgacc gctcgctgga gcgggcccgg gccgccgccg
      841 gttccgccgt cctcggcctg gcggtcggag cccgaccgac gatggtggtg acggcgatcc
      901 tcctggccgt cgtcgcggcg gtcgtcgtgg cccgtcgggg ttcccggccg accgccagcc
      961 tgatcgccga cctcctggcg gtcgcggggc cgttcgtggt cgtcggcatc tgcatcgcgg
     1021 tcgccaacgc ggtccgcttc ggctcaccca ccgagttcgg gttcggcttc cagctctcgg
     1081 tgtgggacat gacgacctac ccgcagggtc gcctgtcgta cctggcaccg aacctgctcg
     1141 accacatcgc tgcgatcccc ggtcaccgga cgtcgttccc gtggatcaca ctgcgcccca
     1201 cgatcggcgg cgaccgaccg accgtccaca cgtccgaacc gatgatcggg ctgatcttcg
     1261 gcgctcctgt cctggtcgtc ggggcggtcg cggctctgcc atcaggccgg gcgccgtggg
     1321 cgagggcgcg ggggctcggc accgcggtgg ccgccgctgc caccacgggg gcgctactgc
     1381 tcgtcctggt ctcgtggccg ttcaacacct ccagcctgcg atacacagcg gacggcgctc
     1441 ccctgctgct gctcgcagca gccggggcgt ggctcacggt ccgctcggac gcaccgctcg
     1501 cgtcgggcac gggagcgggc accggcggcc gccggctgga ccgggcatgg ctggtcgcac
     1561 tcgcggtggg gatcgcagtg accgccgcgg tccaggtgcc cacgtaggac ggccgaccgc
     1621 gcgacactcg tccgatgtcc gacgcctccc ccctgatgac cggccccctc ggcgagccgt
     1681 tggagttcac ctgcccgcgc tgccagcgtc ccgcggtcga gccgttctac gggccgtgcg
     1741 gcacgtgtcg caccacgctg cgggcggtga tgggcggaga ggcgcaggag gtcgagaagg
     1801 tcgactacga gccgaagatg aacgtcaccc ccaacgccgt cgccaccaag gactgagccc
     1861 gagcagaccg ccccgtcagt ccgtgccggg tcgccggtcc cgctcgtgct cggcgtcgtc
     1921 gccgtcgggc accaccccgc ccgggtaggt gcgggcgagc tcctcgggtg tcggggtgcg
     1981 cacctcgcgg gccgccgcgg gcagcaggtc gacgatcgcc gccatcatcg cctcggtgtc
     2041 agccgcgacg tcctctccac cgaggggcac cggtgcgccg acgcggaccc gcaccgtcgg
     2101 cgggtgcagg acgttggtca gatcggggac cttggcgcta cggggccaca cctgctcggt
     2161 gccccacagc ccgatcggga tcaccggaac gccggtggcg tgggccaggc gcaccgcacc
     2221 ccatcggccc ttgagctcgg ggtcgaagaa cgccggtccg cgggggatgg tcccctgggg
     2281 catgagggcc accagctggc ccgcggcgag ggcctcctcg gcggcccgca gcggggcgtc
     2341 cgatccggtg ccacgatcga cgcggatccc tccgagcgcc gtggcgacct cacccacgat
     2401 cggagcatcg aacacctcct tcttgccgag gaagcgcacc ggccgtcccc gacgggcgat
     2461 ggtgaagccg atggccagcg ggtccaggta ggagcggtgg ttgcccacca ggatggcggg
     2521 accctcggcc gggatgtggt cgagcccgcc caggtcgaag cgcacccacg ggaacagctg
     2581 gggttgggcg agcatgaaca gaacctgctg cggttcgatg cccacgaact tggggacacc
     2641 ggccggcacg tcgaagtgga cgaccggcca gcgccggacc gtcgccatgc ccagcatgcg
     2701 ggggtcgggg ttcaccacgg tggggtggcc gacgatgctc agcagcgggg tgtcgtacca
     2761 gctgtcggag taggcatagc tcgccagcag gtcgacgtcg ttgtcgagca cccactgcga
     2821 gacggcccgg gccttgcccc gtccccacac gaactccccg tcgatcgtgc cgtcgtagcg
     2881 gccgtcctcg gtgccgtagc gggtggcgac gacgtcgtcg aagcccaggc ggtcggcgag
     2941 cggtcggatc agatcctcgg gggtcgtggt ggccatgacc agcaggcgcc ccgcggcccg
     3001 gtgctcgtcg atgagctgac gtgcgtacgg gagcacggcg tcggccagcg gttcagccgc
     3061 ccaccgggcg gcctctcgga ccgcgtcgac gggccagcct ctcgccaccc gcgctccctg
     3121 ccggctgacc agcatggacg ggtaggtctc gccgatcagg tcgaacacct tgaacatgac
     3181 cgactcgatg ccctgcgtgg agccagacac cactcccgcg cggcgcagcg cctcgctcag
     3241 gatcggaccg ctggcaccga tcagcagcgt gcgatcgaga tcgaagatgg ccgcggcgcg
     3301 gcggtccgac gatccgctg
//
LOCUS       DAOQOD010000263         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14431_length_3296_cov_9.179883, whole genome shotgun sequence.
ACCESSION   DAOQOD010000263 DAOQOD010000000
VERSION     DAOQOD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14431_length_3296_cov_9.179883"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLZ72_15585"
     CDS             <1..1235
                     /locus_tag="PLZ72_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37771.1"
                     /translation="LRSGGAAVARWPQDFAAAAGGSTVVGGRSAVFAPLPALSAVVVF
                     EEDDPGLQNESSPTWNAREVAIERARRAGVPCLLVSPCPSVVARSAADRATSLGTRTA
                     RHGWAPLVVVDRRDEDVGRSGLFSPTFVEVARDVLGSGGRVVCVLNRTGRARLLACRS
                     CGTVTTCERCDAAVHLSDRLDLECSRCGARRPQVCLPCGSTALSLLRPGVTRAREELE
                     ALLREPVGLVTASSPADVGGRALIGTSAVLHRTHGAGLVAFLDVDQELLSVGYRAAED
                     ALRLLALASRAVAEPAVVDGGRPGRVLVQTRRPDHVVLRAARQGDPALVSAVEAPTRE
                     LLGYPPSATIAVIGGVAGAEFVHRLGAPLGVEVLGPDERGNWLARSEDRDVLLDALRA
                     VERPPGRLRLWVDPVRTR"
     gene            complement(1259..1936)
                     /locus_tag="PLZ72_15590"
     CDS             complement(1259..1936)
                     /locus_tag="PLZ72_15590"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNA37772.1"
                     /translation="MSPPSRPVPPAAPNGGQYFAERPAASSRPGRVELTLPDVHLRLD
                     TDAGVFSPGRVDPGTKLLLTELPPATEWPEGDVADVGCGYGAIAVTLARRLADGTTGA
                     ADGRDRTVWAVDVNERARDLCRRNAAGSGVGELVRVVGPEDLPPGLSVGLIASNPPIR
                     VGKSVLHDLCRSWMERLGPDGEAWWVVQKHLGSDSLQAWMNAQGWPTERVRSRQAYRI
                     LRSTHPG"
     gene            1962..2528
                     /gene="def"
                     /locus_tag="PLZ72_15595"
     CDS             1962..2528
                     /gene="def"
                     /locus_tag="PLZ72_15595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNA37773.1"
                     /translation="MSAPYDIRIVGDPVLTQRAAEVSDIDGRLVKLVDDMLDTMYDAP
                     GVGLAAPQVGVQKRFFVYDLGEGDGGQVLINPEIRESDGEWEFTEGCLSVPGLHWDIV
                     RPKRIHVTGIDLDGATVDFEADEFFARVIQHELDHLDGVLLLDRLDEDQRKDAKRAVR
                     ELQMRFSEPAEACLTPEPRSRTSFFRLR"
     gene            2549..>3296
                     /locus_tag="PLZ72_15600"
     CDS             2549..>3296
                     /locus_tag="PLZ72_15600"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNA37774.1"
                     /translation="MRLAFLGSPPAAVPTLTALVSAGHDVALVVSGPDKRRGRGAAKG
                     PTPVKAAAVELGLPVTDRLEDVVDAGVDLGVVVAYGRIIPTAVLDAVPMVNVHFSLLP
                     RWRGAAPVERAILAGDAVTGVCVMDVAEGLDTGDVHARAEVPVDDQVTAVELTAELAR
                     VGAELMVDALQGGLDAGRPQSATGVTYAHKITAEDRRLDWMLPAVDVARIVRIGGAWT
                     TFRGERFKVVSAEPVDVDVVPDGPGAIDGTD"
BASE COUNT          385 a         1218 c         1228 g          465 t
ORIGIN      
        1 cgctgcggag cgggggagcg gcggtggccc gctggcccca ggacttcgcc gccgcggccg
       61 ggggatcgac ggtcgtggga gggcgctcgg cggtcttcgc tccgctgccg gcgctgagcg
      121 cggtggtggt gttcgaggag gacgatccgg gtctgcagaa cgagtcgtcg ccgacctgga
      181 acgcccgcga ggtcgccatc gagcgggccc gtcgggccgg ggttccctgc ctgctggtct
      241 cgccgtgccc gtcggtggtg gcgcgctccg cggctgaccg agcgacgtcg ctcggcaccc
      301 ggacggcccg ccacgggtgg gcgcccctgg tggtggtcga ccgtcgggac gaggacgtgg
      361 gacgcagcgg tctgttctcg ccgaccttcg tcgaggtggc ccgtgacgtg ctcggctccg
      421 gcggccgtgt cgtctgcgtg ctcaaccgga ccggccgtgc ccgtctgctc gcgtgccgca
      481 gctgcgggac ggtcaccacc tgcgagcgct gcgacgccgc ggtgcacctg agcgaccgcc
      541 tcgacctgga gtgctcccgc tgcggggcgc ggcgtccaca ggtgtgcctc ccgtgcggct
      601 ccaccgccct gtcgctgctg cgccccggtg tcaccagggc ccgcgaggag ctcgaggcgc
      661 tgctgcggga gcccgtcggc ctggtcaccg cgtcgtcgcc ggccgatgtc ggcggtcggg
      721 cgctgatcgg tacgagcgcc gtgctgcacc ggacccacgg ggcaggtctc gtggcgttcc
      781 tcgacgtcga ccaggagctg ctctcggtcg ggtaccgggc cgccgaggac gcgctgcgcc
      841 tgctggcgct ggcgtcgcgg gcggtggcgg aaccggccgt ggtcgacggg gggagacccg
      901 ggcgggtgct ggtgcagacc cgtcgccccg accacgtggt gctgcgggcc gcccggcagg
      961 gggacccggc gctggtctcc gcggtcgagg cgccgacgcg cgagctgctg ggctaccccc
     1021 cgtcggcgac gatcgccgtc atcggcgggg tggccggagc ggagttcgtg caccgcctcg
     1081 gtgcgccgct gggcgtcgag gtcctcggac cggacgagcg gggcaactgg ttggcccgct
     1141 ccgaggaccg cgacgtcctt ctcgacgccc tccgagcggt ggaacgaccg ccgggccggc
     1201 tccgtctctg ggtcgacccg gtacggaccc gctgaccgcc ggcgtccggc cgtcgggctc
     1261 agcccgggtg ggtcgagcgg aggatccggt aggcctggcg ggatcgcacc cgctcggtcg
     1321 gccagccctg ggcgttcatc cacgcctgca gcgagtccga cccgaggtgc ttctgcacca
     1381 cccaccacgc ctcgccgtcg ggtcccaacc gctccatcca ggaccggcac aggtcgtgca
     1441 ggacggactt gccgacccgg atcggggggt tcgacgcgat caggcccacc gacagccccg
     1501 gcggaaggtc ctcggggccg acgacccgca cgagctcacc gaccccggat cccgccgcgt
     1561 tgcgccggca caggtcccgc gcccgttcgt tgacgtcgac cgcccacacc gtccggtccc
     1621 ggccgtcggc ggctcccgtt gtgccgtcgg cgagcctccg cgccagcgtc accgcgatcg
     1681 cgccgtagcc gcagccgacg tcggcgacgt ctccctccgg ccactccgtt gccggaggga
     1741 gctcggtcag cagcagcttg gtccccgggt cgacccgtcc gggggagaac acgccggcgt
     1801 cggtgtcgag tcgcaggtgg acgtcgggca acgtcagctc gacccgcccg ggacgggacg
     1861 acgcagccgg gcgctcggcg aagtactgac cgccgttcgg agccgcgggg ggcacggggc
     1921 ggctcggggg cgacacgacc gtcacggtag ccttggccac catgtccgcc ccctacgaca
     1981 tccgcatcgt cggcgacccg gtgctcaccc agcgcgccgc ggaggtctcc gacatcgacg
     2041 ggcggctggt caagctggtc gacgacatgc tcgacaccat gtacgacgcc cccggggtcg
     2101 gtctcgccgc cccgcaggtg ggggtccaga agcgcttctt cgtctacgac ctcggcgagg
     2161 gggacggcgg ccaggtgctg atcaaccccg agatccgcga gagcgacggc gagtgggagt
     2221 tcaccgaggg ctgcctctcg gttcccggcc tgcactggga catcgtgcgg cccaagcgga
     2281 tccacgtcac cgggatcgac ctcgacggcg ccacggtcga cttcgaggcc gacgagttct
     2341 tcgcccgggt gatccaacac gagctcgacc acctcgacgg cgtcctgctc ctcgaccgtc
     2401 tcgacgagga ccagcgcaag gacgccaagc gggccgtccg cgagctgcag atgcggttct
     2461 cggagcccgc cgaggcgtgc ctcaccccgg agccgaggtc ccggacgtcg ttcttccggc
     2521 tccgctgagc tccgatccgc ccatcccgat gcgtctcgcc ttcctgggca gccccccggc
     2581 cgcggtaccc accctgacgg ccctggtctc cgccggtcac gacgtcgctc tggtcgtgtc
     2641 gggccccgac aagcggcgcg gtcggggtgc ggccaagggg ccgacccctg tcaaggcggc
     2701 cgcggtcgag ctcggcctgc cggtcaccga tcggctcgag gacgtcgtcg acgccggcgt
     2761 cgacctcggc gtggtcgtcg cctacggccg gatcatcccg acggcggtgc tcgacgccgt
     2821 ccccatggtc aacgtccact tctcgctcct gccccggtgg cggggagcgg cgccggtcga
     2881 acgggcgatc ctggccggcg acgccgtgac cggcgtctgc gtcatggacg tcgccgaggg
     2941 cctcgacacc ggcgacgtcc acgcccgggc cgaggtccct gtcgacgacc aggtcaccgc
     3001 ggtcgagctc accgccgagt tggcgcgcgt cggcgccgag ttgatggtgg acgcgctgca
     3061 gggcggcctg gacgccggtc gtccgcagtc ggcgacggga gtcacctacg cccacaagat
     3121 caccgccgag gaccggcgtc tggactggat gctgccggcg gtcgacgtgg cacggatcgt
     3181 ccgcatcggt ggggcgtgga cgacctttcg cggtgagcgg ttcaaggtcg tctccgccga
     3241 gccggtcgac gtggacgtcg tcccggacgg tcccggggcc atcgacggca ccgacg
//
LOCUS       DAOQOD010000264         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14891_length_3241_cov_11.087884, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000264 DAOQOD010000000
VERSION     DAOQOD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14891_length_3241_cov_11.087884"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1116
                     /gene="tal"
                     /locus_tag="PLZ72_15605"
     CDS             19..1116
                     /gene="tal"
                     /locus_tag="PLZ72_15605"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNA37775.1"
                     /translation="MGRLQDLHSEQGQSPWLDNLKRSLLVDGGMQRWIDRGIRGVTSN
                     PSIFQKAIESSDDYTAQLADLVRAGASIEDAYWSMVTDDVTAALELFRPLYDASDGGD
                     GYVSVEVAPSLARDTDATVEMTRELWRRIDRPNLFVKIPATAEGVPAIRQAVSEGINV
                     NVTLIFGLDRYASVIEAFIDGIDAASDAGLDVGRIASVASFFISRVDTEVDRRLESVG
                     TEQALALRGQAAVAQGQLAGQMARDAFAGPRWEALAARGARVQRPLWASTSTKNPAYP
                     DTLYVDRLIGPDTVNTIPEATLDAFEDHGTLARTLDDDVEGARATWAAVGELVDLDDV
                     SKVLEVEGVAAFEKSFDELLGVLTTRAGDLA"
     gene            1240..1788
                     /locus_tag="PLZ72_15610"
     CDS             1240..1788
                     /locus_tag="PLZ72_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNA37776.1"
                     /translation="MGPAADLAPPPAGTRSSRSDRTDPAGSTELADVADPTCSIARAL
                     GIVGDRWTILILRDAFRGLRRFDELQRDLEIPRAVLSDRLRKLVDAGVLTRRRYQDHP
                     PRDEYRLTSMGLQLSPILVALMQWGDRWLSGPDGAPTLLVHEPCGTEVDMGFHCATCG
                     TDFTPTDIASRPGPGRGTGATA"
     gene            1785..>3241
                     /gene="cofG"
                     /locus_tag="PLZ72_15615"
     CDS             1785..>3241
                     /gene="cofG"
                     /locus_tag="PLZ72_15615"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HNA37777.1"
                     /translation="MMEVTGLPLDELMAQARQVRDLTTGTRITYSPKVFIPLTMLCRD
                     RCGYCTFAQPPARLDSPFLEPDQVLRIARAGARAGCHEALFTLGEAPEDRYPVAAEWL
                     AERGYGSTVDYLAAMAERVLDETGLLPHANAGALGVDDLARLRTVSPSQGMMVESLNG
                     DLDAHRGAPDKDPARRLATLEAAGELAIPFTTGILVGIGESRQDRIDALEAIAESHRR
                     HGHVQEVIVQNFLPKAGTAMHQSPPCPPDEHLDAIALARLILPTEVHVQAPPNLSDDF
                     GVLLDAGIDDWGGVSPLTADHVNPERPWPALERLREVTEARGFALAPRLTAHPEFVRD
                     PDRWIDPGLHFAVLDRSDAEGLGRDDPGAQFPQKVESITPVGDDAEVTLRGPRSTQWY
                     SGAPVAPPLLVPAPHDAVTGAVAEVLDGVRSGQEVGEEQILALFSARGPEVATVAAVA
                     DDLRREIVGDEVTFVRNRNINYTNVCTFKCRFCGFS"
BASE COUNT          462 a         1240 c         1095 g          444 t
ORIGIN      
        1 ggggggtagg ttggcgccat gggacgcctc caggacctcc actccgagca gggccagagc
       61 ccgtggctcg acaacctcaa gcgcagcctc ctcgtcgacg gcggcatgca gcgctggatc
      121 gaccgcggca tccggggtgt cacgtccaac ccgtcgatct tccagaaggc gatcgagtcg
      181 agcgacgact acacggcgca gctcgccgac ctggtgcggg ccggtgcgtc gatcgaggac
      241 gcctactgga gcatggtcac cgacgacgtg accgcggcgc tggagctgtt ccggccgctg
      301 tacgacgcgt cggacggcgg tgacggctac gtctcagtcg aggtcgcgcc gtcgctggcg
      361 cgcgacaccg acgcgaccgt cgagatgacc cgggagctgt ggcgtcgcat cgaccggcca
      421 aacctcttcg tcaagatccc cgccaccgcc gagggcgtcc ccgccatccg acaggcggtg
      481 tcggagggga tcaacgtcaa cgtgacgctg atcttcggcc tggaccgcta cgcctcggtc
      541 atcgaggcct tcatcgacgg catcgacgcc gcgtcggacg ccggcctcga cgtcggccgc
      601 atcgcgtcgg tggccagctt cttcatcagc cgcgtcgaca ccgaggtcga ccgtcgcctc
      661 gagtcggtcg gcaccgagca ggccctcgcc ctgcggggtc aggccgccgt cgcccagggc
      721 cagctcgccg ggcagatggc acgcgacgcc ttcgccggtc cccgctggga ggcgctcgca
      781 gctcggggtg cccgggtcca gcggccgctg tgggcctcga cgtcgaccaa gaaccccgcc
      841 taccccgaca cgctctacgt cgaccgcctg atcggccccg acacggtcaa caccatcccc
      901 gaagccaccc tcgacgcgtt cgaggaccac ggcacgctcg cccgcacgct cgacgacgac
      961 gtcgagggcg cccgggcgac gtgggccgcg gtcggcgagc tggtcgacct ggacgacgtg
     1021 tcgaaggtcc tcgaggtcga gggcgtcgcc gcgttcgaga agagcttcga cgagctcctc
     1081 ggcgtcctga cgacccgggc cggcgacctg gcctgatccc cggcctccgc gtgcgggagc
     1141 gacgcgtggc gcgtcggttc cacacgcgga cacggttcct gcgggcgagt gcgttcggag
     1201 tgagtgcgtt cgggaaatga acgcactagg gtgaccggga tgggacccgc cgccgacctg
     1261 gcacctcccc ccgcaggaac ccgctcgtcc cgctcggaca ggacggaccc ggccggctcg
     1321 accgaactgg cggacgtcgc ggatccgacg tgctcgatcg cccgggcgct cggcatcgtc
     1381 ggcgaccgct ggacgatcct gatcctgcgc gacgccttcc ggggcctgcg tcggttcgac
     1441 gagcttcagc gggacctcga gatcccccgg gccgtgctgt cggaccggct gcggaagctg
     1501 gtcgacgccg gcgtcctgac ccgccgccgc taccaggacc acccgccccg cgacgagtac
     1561 cggttgacgt cgatgggcct ccagctctcg cccatcctcg tggcgctcat gcagtggggc
     1621 gaccggtggc tcagcggccc cgatggcgcg ccgacccttc tggtgcacga gccgtgcgga
     1681 accgaggtcg acatgggctt ccactgcgcc acctgcggca ccgacttcac ccccaccgac
     1741 atcgcgagcc ggcccgggcc cggtcgtggg accggggcga ccgcatgatg gaggtgacag
     1801 ggctgccgct cgacgagctg atggcgcagg cccgccaggt ccgcgacctc accaccggga
     1861 cccgcatcac ctactcgccc aaggtgttca taccgctgac gatgctgtgt cgggaccggt
     1921 gcggctactg caccttcgcc cagccccccg cccggctcga ctcgccgttc ctcgagccgg
     1981 accaggtgct gcgcatcgcc cgcgccggcg cccgtgcggg gtgccacgaa gcgctgttca
     2041 cgctgggtga ggcgcccgag gaccgctacc cggtcgccgc ggagtggctg gccgagcggg
     2101 gatacgggtc gaccgtcgac tacctggcgg ccatggccga gcgggtgctg gacgagaccg
     2161 gcctgctccc ccacgccaac gccggggcgc tcggcgtcga cgacctggcc cgcctgcgga
     2221 ccgtctcgcc gtcgcagggg atgatggtcg agtcgctcaa cggcgatctg gacgcccacc
     2281 gtggcgcacc cgacaaggac ccggcccgcc gcctcgccac actcgaggcc gccggcgagc
     2341 tcgccatccc gttcaccacc gggatcctgg tcggcatcgg cgagtcacga caggaccgca
     2401 tcgatgcact cgaggccatc gccgagtcgc accgtcgcca cggtcacgtc caggaggtga
     2461 tcgtccagaa cttcctgccc aaggccggca ccgcaatgca ccagagccca ccgtgccctc
     2521 ccgatgagca cctcgacgcg atcgccctgg ctcgactcat cctgcccacc gaggtccacg
     2581 tccaggcccc gccgaacctc tccgacgact tcggggtcct gctcgacgcc ggcatcgacg
     2641 actggggcgg cgtgtcgccg ttgaccgcag accacgtcaa ccccgaacgg ccctggccgg
     2701 cgctggagcg actgcgagag gtgaccgagg cgcggggctt cgccctggcc ccccgcctga
     2761 ccgcgcaccc cgagttcgtc cgcgaccccg accgttggat cgacccgggc ctgcacttcg
     2821 cggtgctcga ccgctccgac gccgaggggc tgggccgcga cgaccccggg gcgcagttcc
     2881 cgcagaaggt cgagtcgatc acgccggtcg gcgacgacgc cgaggtcacc ctccgcggac
     2941 cccggtccac ccagtggtac tcgggggcgc cggtcgcccc tccgctgctg gtccccgccc
     3001 cgcacgacgc cgtcaccgga gcggtcgccg aggtgctcga cggcgtccgg tccggccagg
     3061 aggtcggcga ggagcagatc ctcgccctct tctccgcccg cggccccgag gtcgccaccg
     3121 tcgccgcggt cgcagacgat ctgcgacggg agatcgtcgg cgacgaggtc accttcgtcc
     3181 gcaaccgcaa catcaactac acgaacgtct gcacgttcaa gtgccggttc tgcggcttct
     3241 c
//
LOCUS       DAOQOD010000265         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14914_length_3238_cov_9.399309, whole genome shotgun sequence.
ACCESSION   DAOQOD010000265 DAOQOD010000000
VERSION     DAOQOD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14914_length_3238_cov_9.399309"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1670
                     /locus_tag="PLZ72_15620"
     CDS             309..1670
                     /locus_tag="PLZ72_15620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNA37778.1"
                     /translation="MLVATAAATLVAAAAVAVVIGALVVDGPDAVVVAAAQLAVLVPA
                     VAVAVTLLAMVRNRLRDQIDEPVAELRQVAAAVLDGDLTVRTGRAGPTDVDALAEELD
                     AAVGSVQVHIRRLRQHAEWGAQSRMIFEALDLADDESGAHDVARQALAIVDPERSVEL
                     LLAPHGSTELTVVATNPNQPAPGCPVDEVSGCIAIRRAQPVVFDSSESINACPKMRGR
                     PYGPCSGACVPVSVAGRPIGVLHTVAADRHPPGERLVGQLATLATQLGNRLGALRALE
                     SSRLEAATDKLTGLPNRRLLESQLASLIERSTPFVLVLADLDNFKHLNDTYGHEIGDR
                     ALQLFAQVLRDKVRGNDVIARLGGEEFVLVYPNMSVTTSIEAVERVRDGLTQTLAVSA
                     VPEFTCSFGITHSSVGRDSESILRVADAGLLRAKELGRDQVVYADETLAARMFGEGDD
                     RRR"
     gene            complement(1674..2702)
                     /locus_tag="PLZ72_15625"
     CDS             complement(1674..2702)
                     /locus_tag="PLZ72_15625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNA37779.1"
                     /translation="MCSEDIPRGLVALPEASRRRFLGLSGAVGGAVTAAALTGCTEDV
                     DQPARGRLSPDEAIIEHTYTGLSTRSEGMTLRQLVESSDAAAPDNQLRGVHRMLWDVG
                     TQKPYVALSFDDGPDPRWTPRVLDALRDAGAKATFFLMGYNVDHHRELAHRVLDEGHD
                     IGNHTWSHQDLAFQDPASTREEIVGGHRAIKEILGVDTNWFRPPRGELSGAGLRILAE
                     ENYDTFLWSVSRNVPGVGTPAEVARHIVERLRPGAIVDMHDSIGRGLFLPQSDRIVET
                     LKAKRDVDVDAVPAILEGGMSAGLEFVTMSTLLAAADAPGALTPATPEDQNLSQGPTT
                     VPPRTATA"
     gene            complement(2798..>3238)
                     /locus_tag="PLZ72_15630"
     CDS             complement(2798..>3238)
                     /locus_tag="PLZ72_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNA37780.1"
                     /translation="GGGGGGGGGGVPATITGSGEIVSVGGIRVHQSIAGNLQALLNAA
                     SAAGINLSGGGYRDPAGQIAVRRNNCGSSNYAIYEAPASSCSPPTARPGSSMHERGLA
                     IDFTQGGSTLSRGSSGFAWLKANAGSFGFRNLPSEPWHWSTNGN"
BASE COUNT          405 a         1172 c         1187 g          474 t
ORIGIN      
        1 cggccgcgtc cacgccggcc gcgcccgcct ccacggggga ggtgctctcc gggcatccaa
       61 ctgcccccac cggtgtcgct ccctcgcccg gtgtcgggga gcccgatcct ccggccggct
      121 cgagcggagc ttccacctcg ggagtggtgc acgtccgcga tgagacgatc ggcctgtccg
      181 gactccccac aggggcgtcc ccgtccgcac acgtcgtcga cccgccggtg cctcgccccg
      241 gagtcgcggc aggcggggat gggaccggtc cggtgcggga ccgtcggccg gcgtcgcgat
      301 cggtccgcgt gctcgtcgcc accgcggccg cgacgctggt cgcggccgcg gctgtcgcgg
      361 tggtcatcgg cgcgctggtc gtcgatggtc ccgacgcggt ggtcgtcgcg gctgcccagc
      421 tggcggtcct cgtcccggcg gtggccgtgg ccgtcacgtt gctggcgatg gtccgcaacc
      481 gtctgcgcga ccagatcgac gagccggtcg ccgagctccg ccaggtcgcc gcggccgtcc
      541 tcgacggcga cctgaccgtc cgcaccggca gggccggacc gaccgatgtc gacgcgctcg
      601 ccgaagagct cgacgccgcg gtcgggtcgg tccaggtgca catccggcgg ttgcgccagc
      661 acgctgaatg gggcgctcag agccggatga tcttcgaggc cctcgacctg gccgacgacg
      721 agtccggagc ccacgacgtc gcccgtcagg ccctggcgat cgtcgatccc gagcgatcgg
      781 tcgaactgct gctcgcgccg cacggctcga cggagctgac cgtcgtggcg accaacccga
      841 accagcccgc tccggggtgc ccggtcgacg aggtgtccgg ttgcatcgcc atccgtcggg
      901 cccagccggt cgtcttcgac agctccgagt cgatcaacgc gtgccccaag atgcggggtc
      961 gtccgtacgg tccgtgctcg ggggcgtgcg tgccggtgag cgtcgccggc cgcccgatcg
     1021 gggtcctgca caccgtcgcc gccgaccgcc acccgccggg cgagcggctg gtcggtcagc
     1081 tcgccacgct ggccacccag ctcggcaacc ggctgggagc gctgcgggcc ctcgagagct
     1141 cgcggctcga ggccgccacc gacaagctca ccgggctccc gaaccggcgt ctgctcgagt
     1201 cgcagctggc gtcgctgatc gagcggtcga cgccgttcgt cctggtcctc gccgacctcg
     1261 acaacttcaa gcacctcaac gacacctacg gccacgagat cggcgaccgc gccctgcagc
     1321 tcttcgctca ggtgctgcgc gacaaggtcc ggggcaacga cgtcatcgcc cgcctcggcg
     1381 gcgaggagtt cgtgctcgtc taccccaaca tgtcggtcac cacatcgatc gaggcggtcg
     1441 agcgggttcg cgacgggctc acccagacgc tggcggtgtc ggcggttccg gagttcacgt
     1501 gctccttcgg catcacccac tcctcggtgg gtcgggactc ggagtcgatc ctgcgggtgg
     1561 ccgacgccgg gctgctgcgg gccaaggagc tgggccgcga ccaggtcgtg tacgcggacg
     1621 agaccctggc ggctcgcatg ttcggcgagg gcgacgaccg ccgtcgctga ccgtcaggcc
     1681 gtcgcggtcc ggggcggaac cgtggtcggc ccctgcgaca ggttctggtc ctccggggtg
     1741 gccggcgtga gcgcccccgg cgcgtccgcc gcggcgagca gcgtcgacat ggtcacgaac
     1801 tcgagcccgg cggacatccc tccctcgagg atcgccggca ccgcgtcgac gtccacgtcg
     1861 cgcttggcct tgagcgtctc gacgatgcgg tccgactggg ggaggaacaa cccgcggccg
     1921 atcgagtcgt gcatgtcgac gatcgctccc ggacgcaggc gctcgacgat gtggcgcgcg
     1981 acctcggccg gggttccgac cccgggcacg ttgcgcgaca ccgaccagag gaacgtgtcg
     2041 tagttctcct cggcgaggat ccgcagaccg gcgcccgaca gctctccccg agggggccgg
     2101 aaccagttcg tgtcgacgcc gaggatctcc ttgatggccc ggtggccgcc cacgatctcc
     2161 tcgcgggtgc tcgcggggtc ctggaaggcc aggtcctggt gcgaccacgt gtggttgccg
     2221 atgtcgtggc cctcgtcgag cacccggtgc gccagctcgc gatggtggtc gacgttgtag
     2281 cccatcagga agaacgtcgc cttggcaccc gcgtcgcgca gcgcgtcgag cacccggggc
     2341 gtccagcgcg ggtcgggccc gtcgtcgaag ctgagcgcca cgtacggctt ctgcgtcccg
     2401 acgtcccaca gcatccggtg aacgccacgc agctggttgt cgggcgccgc ggcgtcggag
     2461 gactcgacga gctggcgcag ggtcatgccc tcgcttcgcg tcgacagccc ggtgtaggtg
     2521 tgctcgatga tcgcctcgtc cgggctgagc cgcccacggg cgggctggtc cacgtcctcg
     2581 gtgcagccgg tgagcgccgc ggcggtgacg gcaccgccga cggcgccgga caggcccagg
     2641 aagcggcgcc gactggcctc ggggagggcg accagaccgc gggggatgtc ctcggagcac
     2701 atgggccgag atcgtgtcag ctcgagcggc tccggtgggg gagcgtgctc agggtcagga
     2761 cgtcagcagg ctccggggac ggcgccgccg cgctgcctca gttgccgttg gtcgaccagt
     2821 gccagggctc cgacgggagg ttgcggaacc cgaagctgcc ggcattggcc ttcagccagg
     2881 cgaacccgga gctgccgcgg ctgagggtgg acccgccctg ggtgaagtcg atggccaggc
     2941 cgcgctcgtg catggaggag ccgggccggg cggtcggcgg gctgcacgac gacgccggtg
     3001 cctcgtagat ggcgtagttg ctggagccgc agttgttgcg gcgcaccgcg atctgcccgg
     3061 ccgggtcgcg gtaaccgccg ccgctcaggt tgatgccggc ggccgaggcc gcgttgagca
     3121 gcgcctgcag gttgccggcg atcgactggt ggacccggat gccgccgacg ctgacgatct
     3181 cgccgctgcc ggtgatggtc gccggaacgc cgccaccacc gcctcctcct ccgccgcc
//
LOCUS       DAOQOD010000266         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_14993_length_3229_cov_9.171393, whole genome shotgun sequence.
ACCESSION   DAOQOD010000266 DAOQOD010000000
VERSION     DAOQOD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14993_length_3229_cov_9.171393"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLZ72_15635"
     CDS             complement(<1..1153)
                     /locus_tag="PLZ72_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37781.1"
                     /translation="MVRVPESAPVLTVDEVESVRSRLRRSDEESRRAALAAIDGWDGR
                     VVSPAAADSLLRAVTLAYPWVRNPRVDPASRLARALCAAPRAVDVHAVEAAYLVSGER
                     VRRLLLHLLALRRDDDGVGSLVFLLGPDGPADLLPLPVAGMLAPVLSAERAADLVPVL
                     VHLAGRTGWVWHAADLLEDLVVDGRVSGDQQRSIARGLAPVVASIVAACDRATLPVPA
                     RAKGADPVRDADPARDADPAGTADVAGPADVADPAGSDQLPVRVGHPAGRAGRDQSRA
                     DRFRLHSLVSLMGQLAPTVAEPVLRSVVASADPRVAAVGAVEMVSLDVAVAPDRLDLI
                     ARAPEARAELLDGLRRIGRLEDLAPRWRTTAARAEAELVRWLADDAELGR"
     gene            1254..2789
                     /locus_tag="PLZ72_15640"
     CDS             1254..2789
                     /locus_tag="PLZ72_15640"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNA37782.1"
                     /translation="MDPSVQTDAGPARARATIRGVVWLQALANGLGVIAVWAYFAFLL
                     PGSEGGRMESSTINLVAFGTYTAVMVLLALPVNALLLRRAVSWVRLGNPPTEHQRRLL
                     FTLPTVETLSALVTWIGAAVLFGVINQDVARIGVGLALAGIIVCTLLYLLLEGHFRPL
                     YALALIDADLPEDRRDVLPRLMLAWLMGSAVPLLAIGLNPLITPVPLDGNRLTWLAVI
                     GALAGGVVMAVASVSVSRPLNRVREALRRIEQGDLDTHVPVDDLGELGRLAEGVNDLV
                     AGIREREALREAFGRQVGQTDLAELAMSHDEPTATGQRRTVTVLFVDLRGYTLFAERH
                     SPEDVVGMLNRFFAIVVAVVAREGGWVNKFEGDAALCLFGAPTDDPDHAAKALRAAGA
                     IPRELARADTVLRAGIGVATGEVIAGFIGTPERFEYTVIGDVVNVASRLCDMAKDEPS
                     GVLATRAAVDAAGSPEEWRPGGRLAVRGRREKADVYTLEADRGPWWSRVTPRSVRASR
                     SAP"
     gene            complement(2708..>3229)
                     /locus_tag="PLZ72_15645"
     CDS             complement(2708..>3229)
                     /locus_tag="PLZ72_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37783.1"
                     /translation="PAGPGPAPPAGPDGDPDRTAVYAAETAAAPDGGRRFARFVELER
                     FVGAAMTEPWWEQQFPDAPVSVELHRRSRSATFSAAHVTDDGWDGVIWIRDGSWDMVT
                     VLHELAHVATGSWRDPGRWSAEAGHGPRFVDGLARLWRRHMGLHAYGALRLALTERGV
                     TLDHHGPRSASSV"
BASE COUNT          428 a         1215 c         1193 g          393 t
ORIGIN      
        1 cgcgacccaa ctcggcgtcg tccgcgagcc agcgcaccag ctcggcctcg gcccgtgccg
       61 cggtggtgcg ccaccgcggg gccaggtcct cgaggcgacc gatgcgccgc aggccgtcga
      121 gcagctcggc gcgagcctcg ggggcgcggg cgatcaggtc gagccggtcc ggggcgacgg
      181 caacgtcgag ggagaccatc tcgaccgctc ccacggcagc gacccggggg tccgccgagg
      241 ccacgaccga acgcagcacc ggctccgcca ccgtcggggc gagctgcccc atcagcgaca
      301 cgagcgagtg gagccggaac cggtccgccc ggctctggtc ccgtcccgcc cggcccgccg
      361 gatgcccgac tcggaccggc agctggtcgg agccggcagg gtcggccaca tcagcggggc
      421 cggccacatc ggcggtgccg gcggggtcgg cgtcacgggc ggggtctgcg tcacgaacgg
      481 ggtcggcgcc cttggcccgc gccggcacgg gaagcgtcgc ccggtcgcac gccgccacga
      541 tcgacgccac gaccggagcg agtccccggg cgatcgaccg ctgctggtcc ccgctcaccc
      601 ggccgtcgac gaccaggtcc tcgagcagat ccgccgcgtg ccagacccat ccggtccggc
      661 cggccaggtg caccaggacc gggacgaggt ccgcggcccg ctcggcggac agcaccgggg
      721 ccagcatccc ggcgaccggc aacggcagca ggtcggcggg gccgtccggg ccgagcagga
      781 acaccaacga ccccacgccg tcgtcgtcgc gacgcaacgc caggaggtgc agaagcaggc
      841 ggcgcacccg ctcaccggac accaggtagg cggcctccac cgcatggacg tcgaccgccc
      901 gcggcgccgc gcacagcgcc cgggccagac ggctggccgg gtcgaccctc gggttgcgca
      961 cccacgggta ggcgagggtg accgcccgca gcagggagtc cgccgcagcg ggactcacca
     1021 cgcgtccgtc ccagccgtcg atcgccgcga gagccgcccg ccgggactcc tcatcggaac
     1081 gccgcaggcg ggagcgcacg ctctccacct cgtcgacggt caggaccgga gcgctctccg
     1141 gaacccgcac catgtcacca gggtgcagca tggcgaccgg cccggcaatc cgcaggatca
     1201 cccagggcgg ccgcttccct cagcggtcgg tgacagccgt acactcccgc cacgtggatc
     1261 cgtccgtgca gaccgacgcc ggaccggccc gggcccgggc gacgatccgc ggcgtcgtct
     1321 ggttgcaggc gctggccaac gggctcggcg tcatcgccgt gtgggcctac ttcgcgttcc
     1381 tgctgcccgg ctccgagggc ggccgcatgg agtcgagcac catcaacctc gtcgcgttcg
     1441 ggacctacac cgcggtcatg gtgctgctcg ccctgccggt caacgcgctg ctgctgcggc
     1501 gggccgtgtc ctgggtccgc ctgggcaacc cccccaccga gcaccagcgt cgcctgctgt
     1561 tcaccctgcc gaccgtcgag accctctccg cgctggtcac ctggatcggc gccgcggtcc
     1621 tgttcggggt gatcaaccag gacgtcgccc gaatcggcgt cggcctcgcc ctggccggga
     1681 tcatcgtctg cacactgctg tacctgctgc tcgagggtca cttccggccg ctgtacgcgc
     1741 tggcgctcat cgacgccgac ctgcccgagg accgacgcga cgtgctcccc cggctgatgc
     1801 tggcgtggct gatgggcagc gccgtgccgc tgctggcgat cggcctcaac ccgttgatca
     1861 ccccggtccc gctcgacggc aaccggctga cgtggctcgc ggtgatcggc gcgctggccg
     1921 gcggcgtggt gatggcggtg gcgtcggtgt cggtgtcgcg cccgctgaac cgggtgcgag
     1981 aggcgctgcg acggatcgag cagggcgatc tggacaccca cgtccccgtc gacgacctgg
     2041 gcgagctcgg ccggttggcc gagggggtca acgacctggt cgccggcatc cgcgagcgtg
     2101 aggcgttgcg cgaggcgttc ggacgccagg tcggccagac cgacctggcc gagctggcga
     2161 tgtcgcacga cgagccgacc gcgaccggcc aacgtcggac ggtcacagtc ctcttcgtcg
     2221 acctgcgcgg ctacacgctc ttcgccgagc gtcactcccc cgaggacgtc gtcggcatgc
     2281 tcaaccgatt cttcgccatc gtcgtcgcgg tcgtcgcacg cgagggcgga tgggtcaaca
     2341 agttcgaggg cgacgccgcg ctgtgcctgt tcggagcacc caccgacgac cccgaccacg
     2401 ccgccaaggc gctgcgggcc gccggggcca tcccccgcga gctggcccgg gccgacacgg
     2461 tgctccgggc cgggatcggc gtcgcgaccg gtgaggtgat cgccgggttc atcgggacgc
     2521 ccgagcgctt cgagtacacg gtgatcggcg acgtggtcaa cgtcgcgtct cggctgtgcg
     2581 acatggccaa ggacgagccg agcggcgtgc tggccacccg cgccgcggtc gacgcggccg
     2641 ggagcccgga ggagtggcgg cccggcgggc ggctcgcggt gcggggtcgg cgtgagaagg
     2701 ccgacgtcta cacgctcgag gcggaccgcg ggccgtggtg gtcgagggtg acgccccgtt
     2761 cggtgagggc gagccgcagc gccccgtagg cgtgcaggcc catgtggcga cgccacaggc
     2821 gggcgaggcc gtcgacgaac cgtggaccgt ggcccgcttc ggccgaccag cgacccgggt
     2881 cccgccagga tccggtggcg acgtgcgcga gctcgtgcag caccgtgacc atgtcccagg
     2941 agccgtcgcg gatccatatg acgccgtccc agccgtcgtc ggtgacgtgc gcggcggaga
     3001 aggtggccga ccgcgaccgg cggtgcagct cgaccgacac cggggcgtcg gggaactgct
     3061 gctcccacca cggctcggtc atggccgcgc cgacgaaccg ctccagctcg acgaagcgtg
     3121 cgaaccgacg gccgccgtcg ggcgccgcgg ccgtctcggc cgcgtagacc gcggtccggt
     3181 cggggtcccc gtcgggcccg gccggcggcg cgggacccgg tccggcggg
//
LOCUS       DAOQOD010000267         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_15216_length_3202_cov_10.699714, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000267 DAOQOD010000000
VERSION     DAOQOD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15216_length_3202_cov_10.699714"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLZ72_15650"
     CDS             <1..699
                     /locus_tag="PLZ72_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNA37784.1"
                     /translation="PWIDAKYYAATQVMDEARHVEVFAQYLDTKLDGHYPINTHLKLL
                     LDDIIDDSRWDMTYLGMQIMVEGLALAAFGMALQTTPDPLLKRLLRYVMSDEARHVAF
                     GVLSLQEFYAGLTDAELLERQEFAFEAAVRMRDRFLQQEVWERMGIDVRKALKLFDVP
                     AEVKNEDPFQQLLFSKIVPNCKKLGLLDANDGWLRKRFDVLGVTQFEDWVDTGEEYLE
                     MDAVAQDRAAEGAA"
     gene            complement(711..1454)
                     /locus_tag="PLZ72_15655"
     CDS             complement(711..1454)
                     /locus_tag="PLZ72_15655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNA37785.1"
                     /translation="MRRESFMRKGFALAWVVLVSLVVVLAACTPPPSGSGTPPADVPC
                     SDSDHVVRVHGDSLAFHIARAIDAPGRTVMDRTTARASLSFDLPENAELGLPAVSSIV
                     GTVRDRVDRCGVPEVLVIQGGINDLASNVPVTDIEAAVSDLSDWLEARDVPTVWMTIH
                     PLPTAGTYMWVQPARQAYNAWLKAGNVHGIVADCVPYLDDPAHPDTMAPAYWWQVDIW
                     GTIEGVHPNQAGYDQLGRCISDALPSTVG"
     gene            1565..2131
                     /locus_tag="PLZ72_15660"
     CDS             1565..2131
                     /locus_tag="PLZ72_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNA37786.1"
                     /translation="MTEVDPPRIDPVRRSEATGEQLAVLEPLGPMGELNIFGTLAVHP
                     KLMRSWLPFGGRLLQGSSLPARTREVVILRTAARCGSDYEWGQHVAIGLDAGLTEAEI
                     VACARPPEDHPWSAADRAVLAAVDEVVGDHVISGSTWSQLVDSGLDDRQLIELTMLAG
                     HYAMLAGMLRSVGVLTEGPLPAIGRTEP"
     gene            2176..2964
                     /locus_tag="PLZ72_15665"
     CDS             2176..2964
                     /locus_tag="PLZ72_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNA37787.1"
                     /translation="MVGAGQTPGPRIGNGRATAITFAAHGARLLLVDRDEARALDTAT
                     AAADAAAAAGHDLPEPVVVVTDIASADGPDRVVTAAVDRLGGFDILHNNVGIGAGDAP
                     PHRLSDEDYDRILDVNLRAMWRTCRAAVPVLRAQERSAIVNVSSLASIAAATNLTAYK
                     ISKAGVNALTQNLALTGARHGMRANAVLPGLIDTPMGVDHAAELMGVDRDEYAELRAG
                     MVPLGHQGSAWDVARAALFLASDDAAFITGVCLPVDGGQSARIG"
BASE COUNT          513 a         1120 c         1116 g          453 t
ORIGIN      
        1 ccgtggatcg acgccaagta ctacgccgcg acccaggtca tggacgaggc gcggcacgtc
       61 gaggtgttcg cccagtacct cgacaccaag ctcgacggcc actacccgat caacacccac
      121 ctgaagctgc tcctcgacga catcatcgac gacagccgct gggacatgac ctacctcggc
      181 atgcagatca tggtcgaggg attggctctg gccgcgttcg gcatggccct gcagaccacg
      241 cccgacccgc tcctcaagcg gctgctccgc tacgtgatga gcgacgaggc ccgacacgtc
      301 gcgttcggag tgctgtcgct gcaggagttc tacgccggtc tcaccgatgc cgagctcctc
      361 gaacgccagg agttcgcgtt cgaagcggcg gtgcggatgc gtgaccgctt cctccagcag
      421 gaggtgtggg agcgcatggg catcgacgtc cgcaaggcgt tgaagctctt cgacgtgccc
      481 gcagaggtca agaacgagga cccgttccag cagctcctgt tctccaagat cgtgccgaac
      541 tgcaagaagc tcgggctgct cgatgcgaac gacggatggc tgcgcaagcg gttcgacgtg
      601 ctcggcgtca cgcagttcga ggactgggtc gacaccggcg aggagtacct cgagatggac
      661 gcggtcgccc aggaccgggc tgcggagggc gccgcctgat gatcgggtga tcagccgacg
      721 gtggacggca gggcgtcaga gatgcaccgg ccgagctggt cgtagccggc ctggttcggg
      781 tggacgccct cgatggtccc ccagatgtcg acctgccacc agtacgccgg cgccatcgtg
      841 tcagggtggg ccgggtcatc gaggtagggc acgcagtcgg cgacgatccc gtgaacgttc
      901 ccggccttca accatgcgtt gtacgcctgt cgcgcgggtt ggacccacat gtaggtgccc
      961 gcggtcggaa gggggtggat cgtcatccac acggtgggta cgtcacgcgc ttcgagccag
     1021 tcgctgagat ctgagaccgc cgcttcgatg tcggtcaccg gcacgttgct cgcaaggtcg
     1081 ttgatcccgc cctggatcac gaggacctcc ggaacgccgc accggtccac ccggtcgcgg
     1141 acggttccca cgatcgatga cacggccggc aggcccagct cggcgttctc cgggaggtcg
     1201 aagctcagcg aggcccgagc agtcgtccgg tccatcaccg ttcgcccggg cgcatcgatg
     1261 gcccgggcga tgtggaacgc gagcgagtcg ccgtgcaccc gcacgacgtg atcggagtcc
     1321 gagcacggga catctgcggg cggggtgccc gaacccgacg gaggaggggt gcacgcggcc
     1381 aggaccacga ccaacgacac cagcaccacc caggcgagcg cgaagccctt ccgcatgaac
     1441 gattccctcc tcatcgaccg ctccccgccg ggcccggggc ccgcggtctg tcccgagtgt
     1501 acgtgtccgg tcgcggcgcc gccgcaaccg gcccgagctc ccggtgcacc ggtagcctcc
     1561 gaccatgacc gaagtcgacc caccccggat cgatccggtg cgtcgaagcg aggccaccgg
     1621 agaacagctg gccgtcctgg aaccgctcgg gccgatgggc gagctgaaca tcttcggcac
     1681 cctcgccgtt cacccgaagc tcatgcgcag ctggctcccg ttcgggggac ggctgctgca
     1741 gggaagctcg ctgcccgccc ggacccgcga ggtggtgatc ctgcggaccg cagcgcgctg
     1801 cggctcggac tacgagtggg gccagcacgt ggctatcggc ctggacgccg gactcaccga
     1861 agcggagatc gtcgcctgcg cacggccgcc ggaggaccac ccgtggtcgg ccgccgaccg
     1921 ggcggtactg gcggcggtcg acgaggtggt gggcgaccac gtcatctccg gatcaacgtg
     1981 gtcgcagctg gtcgactccg gtctggacga caggcagctc atcgagttga cgatgctcgc
     2041 cggccactac gcgatgctcg cggggatgct gcggtcggtg ggtgtgctca ccgaaggacc
     2101 tctgccggcg atcggccgca ccgagccgtg accgcgccag ggggtgggga ccgactggca
     2161 ggacggatcg ccgtcgtggt cggagcgggg cagaccccgg ggccgaggat cggcaacggg
     2221 agagccacgg cgatcacgtt cgcggcccac ggggccagac tgctgctggt cgaccgggac
     2281 gaagcccggg cactcgacac cgcgacggcg gccgccgacg cagccgccgc ggccgggcac
     2341 gatctgcccg aacccgtcgt cgtcgtcacc gacatcgcgt ccgcggacgg ccccgaccgc
     2401 gtcgtcaccg cggcggtcga tcggctgggc ggtttcgaca tcctgcacaa caacgtcggc
     2461 atcggcgccg gggacgctcc gccgcaccgg ctgtccgacg aggactacga ccgcatcctg
     2521 gacgtgaacc tgcgggcgat gtggcgcaca tgtcgcgccg cggttcccgt gctgcgggca
     2581 caggagcgca gcgcgatcgt caacgtgtcg tccctggcgt cgatcgccgc ggcgacgaac
     2641 ctgaccgcct acaagatctc gaaggccggg gtgaacgccc tcacccagaa cctcgccctg
     2701 acaggtgccc gccacggcat gcgcgccaac gctgtcctgc cgggtctgat cgacaccccg
     2761 atgggcgtcg atcacgctgc cgagctgatg ggtgtcgatc gggacgagta cgccgagctg
     2821 cgcgccggca tggtcccgct cggccaccag ggttccgcct gggacgtcgc ccgggccgcg
     2881 ctgttcctcg ccagcgacga cgccgcgttc atcactgggg tgtgcctgcc cgtggacggc
     2941 ggtcagtcgg cccggatcgg ctgactccac cggcggtcag cccgccgtga cccaccgggg
     3001 cgcaggatgc ggtaggcatg ccccatggtc cgagaacccg ccgcagacga ccccaccgac
     3061 ccgggctcca gggaagcccc ggagcagggg ccgggtgtgt acgtggcact cgaggacgcg
     3121 ttcgagcgcg acacccgcta catcggctca cgcatcacga gggacggacg cgacggctac
     3181 ccggtcgagc ccggccggta cc
//
LOCUS       DAOQOD010000268         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_16137_length_3106_cov_9.133071, whole genome shotgun sequence.
ACCESSION   DAOQOD010000268 DAOQOD010000000
VERSION     DAOQOD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16137_length_3106_cov_9.133071"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1824
                     /locus_tag="PLZ72_15670"
     CDS             34..>1824
                     /locus_tag="PLZ72_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37788.1"
                     /translation="MAATAVVAATVVVLPASPTQAAGTYVVNTPDDLVDLVPGDGQCL
                     AAGNVCSLRAAIQEANAAPGTATTITVDPSVTTMALTIGGGGDGDGRDDVGDLDIAPN
                     VDVTIVGNRVTGNQFTGVDASAMSDRHFEVPAGADLRLIEVSLFGGHPAGDGGAILVS
                     GSVTVNNSMTATTDTYQALYGNTAGGSGGAVAVRTGGIFKVANVSTATGGTSRVDIDG
                     NVAGGSGGAVFNEGYVHMTGAAGPGMPSHDVYLDGNQAATDGGGIDNRAGAEVVLSCR
                     AFVRFGQAGISGGGMINRGSVTLEGGSFDGNVAAAGGNLFNDTGATVTVDPGCEGSRF
                     TRGFASNTGGAIHNRGSIAVNGTLDMSGEAVLLDPSRLVEAAAGGGLYQEAGDIDVFG
                     ALRGDDLEASGHGGAVAVVGGSLRTLDQGRIELEGNRSADGGGLSVQGGTMNVSILLR
                     NNSAYSNGGNVAVSGGDVALYGPSAVLDGYATDGAGLFISDGVFRAENTTVAGNRRGA
                     SPGVGAAVRATGGAVNLRHVSVIDNEPGLSVSTPATLELHRSLLTRNTGGNCLSDPGT
                     ITGGDFNVADDSSCGPVGTDLSDPTEMSQVA"
     assembly_gap    1825..1924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2127..>3106
                     /locus_tag="PLZ72_15675"
     CDS             2127..>3106
                     /locus_tag="PLZ72_15675"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNA37789.1"
                     /translation="MANEPIRVSVTGAAGQIGYSLLFRIASGAMLGPDQPVILQMLEI
                     TPALGALHGVAMELEDCAFPLLDDMVETDDPDVAFGDAGVALLVGAMPRKAGMERSDL
                     LSANGGIFKPQGEALSRSARKDIKVLVVGNPANTNALIAMSNAADLDPGRFTAMTRLD
                     HNRAMAQIAGKLDAAVADVKKMTIWGNHSTTQYPDLFHCEVGGRNAAQAVGDQAWIEN
                     DFIPTVAKRGAAIIDARGASSAASAANAAIDHIRSWHLGTPEGDWVSMGIPSDGSYGV
                     PEGIISSFPCTCSGGEYSIVQGLEIDEFSRGRIDASVAELQEERDAVSELG"
BASE COUNT          442 a         1106 c         1046 g          412 t
ORIGIN      
        1 tgggcccgag ccgtgcgcgt cgtcgggcgc gcggtggccg cgaccgccgt ggtggccgcg
       61 accgtcgtgg tcctgcccgc gtcgccgacc caggccgccg gcacgtacgt cgtcaacacc
      121 cctgatgacc tggtcgacct cgttcccggc gacggccagt gcctggccgc cggcaacgtg
      181 tgctcgctgc gcgccgcgat ccaggaggcc aacgccgctc ccggcaccgc taccacgatc
      241 accgtcgacc cgtcggtcac caccatggcc ctgaccatcg gcggcggcgg cgacggcgac
      301 ggccgtgacg acgtcggcga cctggacatc gccccgaacg tcgacgtcac catcgtcggc
      361 aaccgtgtga ccggcaacca gttcaccggc gtcgacgcgt cggcgatgtc cgaccgccac
      421 ttcgaggtgc ccgccggcgc cgacctccgc ctgatcgagg tctccctctt cggtggacac
      481 ccggccggtg acggcggggc gatcctcgtc tcggggtctg tcacggtgaa caactcgatg
      541 accgcgacga ccgacacgta ccaggccctc tacggcaaca ccgcgggcgg ctccggcggc
      601 gcggtggcgg ttcggaccgg cgggatcttc aaggtcgcca acgtgtcgac ggccaccggt
      661 ggcaccagtc gcgtcgacat cgacggcaac gtcgccgggg ggtccggcgg agcggtgttc
      721 aacgagggct acgtgcacat gaccggtgcc gctggccccg ggatgccgtc ccacgacgtg
      781 tacctggacg ggaaccaggc ggcgaccgac ggtggcggca tcgacaaccg ggccggagcc
      841 gaggtcgtcc tgtcgtgtcg ggcgttcgtg cggttcggtc aggccggaat ctccggcggc
      901 ggaatgatca accgcggatc ggtaaccctc gagggcggat cgttcgacgg gaacgtcgcg
      961 gccgcgggcg gcaacctgtt caacgacacc ggcgccaccg tcaccgtcga tcccggatgc
     1021 gagggctcgc gcttcacccg gggcttcgcc agcaacaccg gcggagcgat ccacaaccgg
     1081 gggtcgatcg cggtgaacgg caccctcgac atgagcggcg aggctgtcct tctcgacccg
     1141 agtcggctgg tcgaggcggc tgccggcggc ggcctctacc aggaggccgg cgacatcgac
     1201 gtgttcggcg ccctccgggg tgatgacctg gaggcatcgg gtcacggcgg cgcggtggcc
     1261 gtcgtgggcg ggtcgctgcg cacgctggac cagggccgga tcgagctcga gggcaaccgg
     1321 tcagccgacg gcggcggcct gtcggtgcag ggcggcacca tgaacgtgtc gatcctgctg
     1381 cgcaacaact cggcgtactc caacggcggg aacgtcgcgg tcagcggcgg cgacgtcgcg
     1441 ctgtacggac ccagcgccgt gctcgacggg tacgccaccg acggcgccgg cctgttcatc
     1501 agcgacggtg tgttccgcgc cgagaacacg accgtcgccg gcaaccgtcg cggcgccagc
     1561 cccggcgtgg gcgcagcggt gcgagcgacc ggcggcgccg tcaacctccg tcacgtctcg
     1621 gtgatcgaca acgagccggg tctgtcggtc tcgacgccgg cgaccctcga gctccaccgg
     1681 tccctgctga cccgcaacac gggcggcaac tgcctgtcgg acccgggaac gatcaccggc
     1741 ggcgacttca acgtggccga cgactcgagc tgcggcccgg tcggcaccga cctgtcggac
     1801 ccgacggaga tgtcgcaggt cgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnntggccc acacggggac cggcgtgagc ggcccggtcc tggccggtgt gatgctgatc
     1981 gcctgcggcg ccatggtgct ggcggcccgc cgtcgggagc accgcttcgc ctgaccgtgc
     2041 cgtactcggc gggtcggttt cgacccgccg agggggcggt cctaacgtga tcgggtaccc
     2101 ctcaacccaa tccgggagga acccccgtgg ccaacgagcc catccgcgtc tccgtcaccg
     2161 gagcagccgg acagatcggc tactccctgc tgttccgcat cgccagcggc gccatgctcg
     2221 gcccggacca gccggtcatc ctgcagatgc tggagatcac tccggcgctc ggcgcgctgc
     2281 acggcgtggc gatggagctc gaggactgcg ccttcccgct gctcgacgac atggtcgaga
     2341 ccgacgaccc cgacgtcgcc ttcggcgatg caggtgtcgc cctgctcgtc ggcgccatgc
     2401 cccgcaaggc cggcatggag cgctccgatc tgctgtcggc caacggcggc atcttcaagc
     2461 cgcagggcga ggcgctcagc cggtccgccc gcaaggacat caaggtgctc gtggtcggca
     2521 acccggccaa caccaacgcc ctgatcgcca tgagcaacgc cgcggacctc gacccgggcc
     2581 gcttcaccgc catgacccgc ctggaccaca accgggcgat ggcgcagatc gccggcaagc
     2641 tcgacgccgc cgtcgcggac gtgaagaaga tgacgatctg gggcaaccac tcgaccaccc
     2701 agtaccccga cctgttccac tgcgaggtcg gcggccgcaa cgccgctcag gccgtgggcg
     2761 accaggcctg gatcgagaac gacttcatcc cgaccgtcgc caagcggggt gcggcgatca
     2821 tcgacgcccg cggcgccagc tccgccgcgt cggccgccaa cgccgccatc gaccacatcc
     2881 gctcctggca cctgggcacc cccgagggcg actgggtgtc gatgggcatc ccgtccgacg
     2941 gctcctacgg cgtgcccgag ggcatcatct cgagcttccc gtgcacctgc tcgggcggcg
     3001 agtactcgat cgtgcagggc ttggagatcg acgagttctc ccggggccgc atcgacgcct
     3061 cggtcgccga gctccaggag gagcgcgacg ccgtctccga gctcgg
//
LOCUS       DAOQOD010000269         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_16610_length_3058_cov_11.590410, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000269 DAOQOD010000000
VERSION     DAOQOD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16610_length_3058_cov_11.590410"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="PLZ72_15680"
     CDS             complement(<1..1936)
                     /locus_tag="PLZ72_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37790.1"
                     /translation="MALDPNTWTHKTQEAVNAALTSAREHSNPEATPDHLLAALLRQD
                     DGVVLPVVRKLGIEPLTLRNAADEAVAALPTAYGTDTRPSRELVALFDAADGERRDLT
                     DEYLSTEHLLLALFEADQKRAVADRRFGELAREQLLSALAEVRGSRRVTSQNPEDTLE
                     ALEKFGRDLTEAAAAGRLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAVVE
                     GLAQRIVAGDVPESLMGKRVISLDMSSMVAGAKFRGDFEERLQAVLNEIKDSDGQIIT
                     FIDEMHTVVGAGATGEGAMDAGNMLKPMLARGELRMVGATTLDEFRKYIEKDPALERR
                     FQQVFVGEPSVESTIAILRGLKERYEVHHGVDVEDPALVAAAVLSDRYLTGRFLPDKA
                     IDLVDEAAASLSIEIGSVPTEIDVVKRRIDGLEIERVALEKATDAGSVERLAALDAEL
                     ANLRESFDGLNARWQAEKDQADAISEIAERVEALKVEAERAERDGDFTRASEITYGHM
                     PVLERELDAANAALAELQGDSPMLKRTVDEQDIAEVVARWTGVPVARLMEGEMSKLVR
                     LEDVLHERVIGQDDAVSAVSNAIRRSRAGLSDPHRPIGSFLFLGPTGVGKTELARSLA
                     DFLFDDERAMVRIDMSEYMEK"
     gene            complement(1981..>3058)
                     /locus_tag="PLZ72_15685"
     CDS             complement(1981..>3058)
                     /locus_tag="PLZ72_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37791.1"
                     /translation="IATALALVAVGALAFPAVAGAQARHVVVVSDSIFLGAKSPFGAR
                     MQTAGWTVDFDGAVSRSTLAGADAVRSRRATITDSLVISLGANDSGNTSTFRSRVDAV
                     MAAADGVPNVYWLTIREVRPYYAPANQVLRDAATRYPNLHVVDWNAASAGRDGLTSGD
                     GLHLTPAGAGEIAALVGTAVTDGSVPVPAAAPPPPPPEPVAQSVPVDPPVVDPAIAAV
                     GAPTAAGDVQASVPVEARGEVPAFDRSDLVRVSEPIAVGGRPARPATSGPGSGPAVVV
                     VAVMALVASVVILLRRSPWMPFVLRAPSIAVRRSPVTRAELRAARIAGSMDRHPSVAS
                     VPPVPDESPADPGARIVSGSSSQS"
BASE COUNT          435 a         1049 c         1130 g          444 t
ORIGIN      
        1 gcttctccat gtactcgctc atgtcgatgc ggaccatcgc ccgctcgtcg tcgaacagga
       61 agtcggccag gctgcgggcg agctcggtct tgccgacgcc ggtcggcccg aggaacagga
      121 agctgccgat cggacggtgc gggtccgaca ggccggcgcg gctgcgacgg atggcgttgg
      181 acacggccga cacggcgtcg tcctggccga tgacccgctc gtgcagcacg tcctcgaggc
      241 ggacgagctt ggacatctcg ccttccatca ggcgggcgac gggcaccccg gtccagcggg
      301 cgacgacctc ggcgatgtcc tgctcgtcga cggtgcgctt gagcatgggc gagtcgccct
      361 ggagctcggc cagagcggcg ttggcggcgt cgagctcgcg ctcgagcacc ggcatgtggc
      421 cgtaggtgat ctcggaggcc cgggtgaagt cgccgtcgcg ctcggcgcgc tcggcctcga
      481 ccttgagcgc ctcgacccgc tcggcgatct cggagatggc gtcagcctgg tccttctcgg
      541 cctgccagcg ggcgttgagc ccgtcgaagc tctcacggag gttggcgagc tcggcgtcga
      601 gcgcggcgag gcgctcgacc gagccggcgt cggtggcctt ctcgagagcg acgcgctcga
      661 tctcgagccc gtcgatgcgg cgcttgacca cgtcgatctc ggtcgggacc gacccgatct
      721 cgatgctgag gctcgcggcg gcctcgtcga cgaggtcgat ggccttgtcg ggcaggaacc
      781 gcccggtgag gtaccggtcc gagaggaccg cggcggcgac cagggcgggg tcctcgacgt
      841 cgacgccgtg gtggacctcg tagcgctcct tcaggccgcg caggatggcg atggtcgact
      901 cgaccgacgg ctcgccgacg aacacctgct ggaagcggcg ctcgagcgcc gggtccttct
      961 cgatgtactt gcggaactcg tcgagggtcg tggcgccgac catgcgcagc tcgccgcggg
     1021 cgagcatcgg cttgagcatg ttgccggcgt ccatggcacc ctcgccggtg gcgccggcgc
     1081 cgacgacggt gtgcatctcg tcgatgaagg tgatgatctg cccgtcggag tccttgatct
     1141 cgttgaggac ggcctggagc ctctcctcga agtcgccgcg gaacttggcg ccggcgacca
     1201 tcgaggacat gtcgagcgag atgacccgct tgcccatcag cgactccggc acgtcgccgg
     1261 cgacgatgcg ctgggcgagg ccctcgacca ccgctgtctt gccgactccg ggctcgccga
     1321 tgaggacggg gttgttcttg gtccgccgcg acaggacctg gatgacccgc cggatctcct
     1381 cgtcgcggcc gatcacgggg tcgagccgtc cggccgccgc ggcctcggtg aggtcgcgcc
     1441 cgaacttctc gagcgcctcg agtgtgtcct cggggttctg cgacgtcacc cgacggctgc
     1501 cgcgcacctc ggcgagcgcc gacagcagct gctcccgggc gagctcgccg aagcgacggt
     1561 cggcgacggc ccgcttctgg tcggcctcga acagggcgag gagcaggtgc tcggtcgaca
     1621 ggtactcgtc ggtcagatcg cggcgttcgc cgtcggccgc gtcgaacagg gccaccagct
     1681 cacggctggg tcgcgtgtcg gtgccgtagg cggtggggag ggcagcgacc gcctcgtcgg
     1741 ccgcgttgcg cagggtcagc ggttcgatgc ccagcttgcg cacgaccggc aggacgacgc
     1801 cgtcgtcctg gcgcagcagc gccgccagca ggtgatcggg ggtggcctcg gggttgctgt
     1861 gctcgcgggc cgaggtcagg gccgcgttca cggcctcctg cgtcttgtgg gtccaggtgt
     1921 tcgggtcgag cgccatgacg gcagcttacc aacaagtcaa caaagttgct agtgatacac
     1981 tcaactctgc gaactggatc ctgagacgat cctcgccccc ggatcggcgg gtgactcgtc
     2041 gggcaccggc gggaccgacg ccaccgacgg gtgccggtcc atggacccgg cgatgcgggc
     2101 ggcacggagc tcggctcggg tcaccggcga gcggcgaacg gcgatcgacg gcgcccgcag
     2161 cacgaacggc atccacggcg aacggcgcaa gaggatcacg accgacgcga cgagagccat
     2221 gacggcgacc accacgacgg cgggcccgga acccggaccg gacgtggccg gtcgagccgg
     2281 ccgacctccg acggcgatcg gctcggagac gcgcaccagg tcggatcgat cgaacgccgg
     2341 aacctctccc ctagcctcga ccgggaccga tgcctggacg tcaccggccg cagtcggcgc
     2401 accgacagcg gcgatggcag gatccacgac cggcgggtcg accggcaccg actgggccac
     2461 cggttcgggt ggcggaggcg gcggtgctgc ggccgggacc ggcaccgaac cgtcggtcac
     2521 cgcggtgccg accagcgctg cgatctcacc cgcaccggcc ggcgtcaggt gcagaccgtc
     2581 gcctgaggtc aggccgtcac gaccggcgct ggcggcgttc cagtccacga cgtgcaggtt
     2641 cgggtaccgg gtggccgcgt cgcgcaggac ctggttggcc ggcgcgtagt acggccgcac
     2701 ctcgcggatg gtcagccagt agacgttcgg gacgccgtcg gcggcggcca tgacggcgtc
     2761 gacccgactt cggaacgtgc tcgtgttgcc cgagtcgttg gcacccaggc tgatgacgag
     2821 ggagtcggtg atggtggccc gccgggagcg cacggcatcc gctccggcca gcgtcgaccg
     2881 gctgaccgct ccgtcgaagt cgaccgtcca gccggctgtc tgcatgcggg caccgaacgg
     2941 ggacttcgcc cccaggaaga tgctgtcgga gacgacgacg acgtgacggg cctgagcacc
     3001 ggcgaccgcc ggaaaggcca gcgcccccac ggcgaccagg gccagcgccg tcgcgatc
//
LOCUS       DAOQOD010000270         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_16805_length_3041_cov_10.110181, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000270 DAOQOD010000000
VERSION     DAOQOD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16805_length_3041_cov_10.110181"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..639
                     /locus_tag="PLZ72_15690"
     CDS             133..639
                     /locus_tag="PLZ72_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37792.1"
                     /translation="MSPVVAAARTMSAHGLSVKVPAGWDGSIRRAAGDPSDTDIDDVG
                     RLGTTNPVLHVASFPLPAVRGDYGGGAVQAMGPNDVFIALVEFDPDAGTTPLFSAPGL
                     RRPLRVTDFDRSTMHRPLPGASGHQQFFHTRGRAFALYVALGAHRLRASSVPRVDRVL
                     ATLSISPR"
     gene            636..2756
                     /locus_tag="PLZ72_15695"
     CDS             636..2756
                     /locus_tag="PLZ72_15695"
                     /inference="COORDINATES: protein motif:HMM:NF027792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HNA37793.1"
                     /translation="MTDGSGGPAAAVHTVLVVAKSHLDVGFTDLAAAVRERWLRELLP
                     RAVHTAAALRDRGAGPRLCWTTPSWILHEALEDGDPGLRAMVDAAVGAGDLAWHAAPF
                     TTHTELVDRSLFAHGLSISARLDQRFGRRTRAAKMTDVPGHTRAVVSLLADAGVDLLH
                     VGVNPAAASPDVPELFRWRDDAAAPVAHPSEPSDAAGRPDPPAINVMYRSGGYGALQV
                     LGDSGVAVDLWVDGDNDGPPDVDDVVARWDRLRLAHPAAEIRAATLDDVADVVRSVAA
                     ELPVLNGEIGDTWIHGIASDPSKTAAFRETCRRRRSWIDSGAVAADDPALWSASTELL
                     LVAEHTWGLDQKTHWPDTDHWSESALASVRPRPATRRFEWSWSEQRSYLDRFLQRLEA
                     AGRSDLAADARAGMLLTGAAPVSVDGLVALSRPDDGGAIRARLGPLEVAVDPHDGALV
                     ELVVDAEGGGRRDLVGGGRALGRVGHRTYDAADYERWFSTYDAGTRPEDRDWALWDNT
                     KPGLDRSGARSAWYAPDLVGAWSGRRDADGGGPGAEVLVLELSFSDETRSLSAAPPWV
                     LVGYELVDGRPDEVSVWVQWVGKAAARWPESTWCSFVPTVADDAAWTMSKMGEDVSPL
                     DVVSRGARTLHAADAVRHRDGFRLELIDAPLVAPGSPSLLRFEDRLPDLAGGWHVCLY
                     ANVWGTNFPMWCPGDARFRLTLGW"
BASE COUNT          347 a         1140 c         1129 g          425 t
ORIGIN      
        1 gtcgaggcgg gcgttcctca ccggacgcga ccgcgccgac cgcgtcgacg ccgaactggc
       61 cgccgcgggc atccgaccgg gggacccgag gctgtacgaa ccggtcgtcg accccgatgc
      121 cgaggagcgc ccgtgagccc cgtcgtcgcg gccgcacgga ccatgagcgc ccacggtctg
      181 tcggtcaagg tccccgccgg ttgggacggg tcgatccgcc gcgccgcggg cgacccgagc
      241 gacactgaca tcgacgacgt cggccggctg ggcaccacca acccggtgct gcacgtggcc
      301 agcttcccgc ttccggcggt ccgcggcgac tacggcggcg gagccgtgca ggcgatgggg
      361 ccgaacgacg tcttcatcgc gctcgtcgag ttcgatcccg acgccgggac caccccgctg
      421 ttctcggcac cgggcctgcg tcgcccgctg cgggtgaccg acttcgaccg ttcgaccatg
      481 caccggccgc tgcccggcgc cagcggccac cagcagttct tccacacccg gggccgggcc
      541 ttcgccctgt acgtggcgct gggcgcccac cgcctccggg cgtcgtcggt cccccgggtc
      601 gaccgggtgc tcgccaccct gtcgatcagc cctcgatgac cgacggctcc ggcggtcctg
      661 ctgccgcggt ccacacggtc ctcgtcgtcg ccaagtccca cctcgacgtc ggcttcaccg
      721 acctggccgc cgcggtccgc gagcggtggc tgcgcgagct gctgccgcgg gcggtgcaca
      781 cggcggccgc cctgcgcgac cgcggcgccg gtccccggtt gtgctggacc accccgtcgt
      841 ggatcctgca cgaggcgctc gaggacgggg acccgggtct acgggccatg gtcgacgccg
      901 cggtgggggc cggggacctc gcgtggcacg ccgcgccgtt cacgacccac accgagctgg
      961 tcgaccgttc gctgttcgcc cacggcctga gcatctcggc ccgactggac cagcggttcg
     1021 ggcgccggac ccgggccgcc aagatgaccg atgtcccggg ccacacccgg gcggtggtgt
     1081 cgctgctcgc cgacgcgggt gtggacctgc tgcacgtcgg ggtgaacccc gcggctgcct
     1141 ctcccgacgt cccggagctg ttccgctggc gagacgacgc cgctgcgccg gtggcgcacc
     1201 cgtcggagcc gtccgacgcg gcgggccgac ccgatccgcc cgcgatcaac gtcatgtacc
     1261 gctccggcgg ctacggcgcc ctccaggtgc tgggtgacag cggggtcgcg gtcgacctgt
     1321 gggtcgacgg cgacaacgac ggccccccgg acgtcgacga cgtcgtcgcc cgctgggacc
     1381 ggctccgcct cgcgcatccg gccgccgaga tccgcgccgc caccctcgac gacgtggccg
     1441 acgtcgtccg gtcggtggcg gcggagctgc cggtgctgaa cggtgagatc ggcgacacgt
     1501 ggatccacgg catcgcctcg gacccgtcga agaccgccgc gttccgcgag acgtgccgac
     1561 ggcgacggag ctggatcgac tcgggtgccg tcgccgcgga cgacccggcg ctgtggtcgg
     1621 cgtcgaccga gctcctgctg gtggccgagc acacgtgggg cctcgaccag aagacccact
     1681 ggcccgacac cgaccactgg tccgagtcgg cgctggcgtc ggtgcggccc cggcccgcga
     1741 cccggcgctt cgagtggagc tggagcgagc agcgctccta tctggaccgg ttcctgcagc
     1801 ggctggaggc cgccgggcgt tccgacctgg ctgcggacgc acgggcgggg atgctgttga
     1861 ccggagcggc accggtgtcg gtcgacggcc tcgtcgccct gtcccgtccc gacgacgggg
     1921 gagcgatccg tgcccggctg ggcccgctcg aggtggccgt cgatccccac gacggggcgc
     1981 tggtcgagct cgtcgtcgac gcggagggag ggggccgtcg cgacctggtc ggcggcggtc
     2041 gggccctcgg ccgggtgggt caccgcacct acgacgccgc ggactacgag cgctggttct
     2101 cgacctacga cgcggggacc cgacccgagg accgggactg ggccctctgg gacaacacca
     2161 agcccggcct cgaccgcagc ggcgcccggt cggcctggta cgcccccgac ctggtcggtg
     2221 cctggtcggg tcggcgtgac gccgacggcg gcggtcccgg cgccgaggtg ctggtgctcg
     2281 agctgtcgtt ctccgacgag acccgttcgc tcagcgcggc tccgccgtgg gtgctggtcg
     2341 gctacgagct cgtcgacgga cgccccgacg aggtgtcggt gtgggtccag tgggtgggca
     2401 aggccgccgc acggtggccc gagtcgacgt ggtgctcctt cgttccgacc gtggccgacg
     2461 acgccgcctg gaccatgtcg aagatgggcg aggacgtctc gccgctcgac gtggtgtccc
     2521 ggggggcgcg caccctgcac gccgcggacg cggtgcgcca ccgcgacgga ttccgcctgg
     2581 agttgatcga cgcacccctg gtggcgccgg ggtcgccctc gctgttgcgc ttcgaggacc
     2641 ggctcccgga cctggccggc ggttggcacg tctgcctgta cgccaacgtg tgggggacga
     2701 acttcccgat gtggtgtccc ggcgacgccc gcttccgcct caccctcggc tggtgacggc
     2761 cgcgccgtcg gtccagactt catcgatgcc tcgtccccgt cgagtcagca cgtcccggcc
     2821 ccgccgcccg gcgggctcga ccgtgtctga gccggttcac ggcgtgccga gggtgacctc
     2881 ggtggacggg gtggcccggc ccggccgcag cgtcaccgag gtggcggtgg gcgtgctgat
     2941 cgagcgcgac gccgtcggcg gcgaaggccg cttcctgctc accagccggc ccgagggcaa
     3001 ggtctacgcc ggctactggg agttcccggg cggcaagctg g
//
LOCUS       DAOQOD010000271         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_17350_length_2992_cov_8.125638, whole genome shotgun sequence.
ACCESSION   DAOQOD010000271 DAOQOD010000000
VERSION     DAOQOD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17350_length_2992_cov_8.125638"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1080
                     /locus_tag="PLZ72_15700"
     CDS             163..1080
                     /locus_tag="PLZ72_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37794.1"
                     /translation="MSVSRRLAIVLAGGILVLSASVPTSVGAAADPPPITVNPASVAP
                     GGAVTVTGSGCGGGSTVLVGVLDAGGASELAAVAGQSGGAAGWSVKLTLPADVRPGTY
                     PVTARCSSYAGGYDGYDGYSGGGFEYAPGTVAVDPPVSAPEKLDGSLTVDPSTVAGGG
                     TVKVTSSGWQPGEQVTVVLYSTPVTLAMVRAGADGRLDVTVAIPGATPPGAHQIVEFN
                     ATSATAPARTLGGPLQVTAATPGSDSVVPISAPTQNADVAGSVESSSGSGSMAYTGTE
                     PVLLIVVGASILILGAVLLLLQRRRPIDS"
     gene            1088..2482
                     /locus_tag="PLZ72_15705"
     CDS             1088..2482
                     /locus_tag="PLZ72_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNA37795.1"
                     /translation="MADRGRAEPGRSGDAREPTVHPDAIVWTGSARDPGQPFSPATFR
                     LDAEAIAITIHRGVGSGTVVVPLADVVGLSAVDRDDGLPELELETGDGAVGRLRWSDE
                     YTEPFVVALERSGDGRPDAGSERADVRHGTSGTDARDRVLRSAAMLLLVSGLLIGLLG
                     VYVAVLDSRVHRWPVDRPEVDDGRETWLLLGSDSRAAAGSMPDRSVFGSTSEVPGERA
                     DVVLLVQEGDGQRSPRLVLLPRDLLVFRAGHGVDRLALTLLDGPGVTATSVCRSLGVP
                     VDHVVTVRFDGIQRLVDSVGGIDVRSELETRDPNTGLHLRPGRNHLNGAGAVAWARSR
                     HPERLVSGEWLPDLDGESVRQSSQRELLSQLASAMAHRAGRDPVTLQRLIWTSAGSVE
                     TDSGTGPLDLARLAVALLGAEDGGSVAHVVSDAPIPVARLLPEAHRQLDRFRGNRDST
                     TPCPRARISVDGQR"
     gene            2526..2831
                     /locus_tag="PLZ72_15710"
     CDS             2526..2831
                     /locus_tag="PLZ72_15710"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNA37796.1"
                     /translation="MARPQPLTGGDLDAALVGLPGWTVVDGRLHRELRFADFPRAFGF
                     MAAVATVAQAMDHHPDWTNSYASVTVDLMTHDVGGITSLDIQLAQRISDLAGDQRPS"
BASE COUNT          395 a         1056 c         1077 g          464 t
ORIGIN      
        1 cggctacgcc gcctggacat aggggtgctc agcgacacac cttgtcgcaa tccgccccct
       61 agaaccgggt agcgcgcaac cccttgatcc tcatcgctcg gtgacctagg ttgcgccgag
      121 ggacagctgc ggactctgat ccgccgctgg ggagggtgga agatgtccgt gtcgcgacga
      181 ctggcgatcg ttctcgccgg tgggatcctg gtgttgtcgg cgtcggtgcc gacatcggtc
      241 ggtgcagcgg cggatccgcc tccgatcacg gtcaatccgg cgtcggtggc ccccgggggt
      301 gctgtcaccg tgaccgggtc gggctgcgga ggcggttcga cggtactcgt cggcgtgctc
      361 gacgccggcg gagccagcga gctcgcggcc gtcgcgggac agagcggcgg cgcagcgggc
      421 tggtcggtca agctcaccct gccggcagac gtgaggccgg gcacctaccc ggtcacagct
      481 cgctgctcga gctacgccgg cggatacgac ggctacgacg gatacagcgg cggtggcttc
      541 gagtacgcgc caggaacggt cgcggtggac ccaccggtct ccgcgccgga gaagctcgac
      601 ggctccctga cggtcgatcc gtcgacggtg gccgggggcg gcacggtcaa ggtcacgagc
      661 tccggatggc agccgggcga gcaggtcacg gtcgttctct actccacacc ggtgacgctc
      721 gcgatggtcc gggcgggtgc cgacggacgg ctcgacgtga ccgtcgccat ccccggcgcg
      781 acccctccgg gcgcccacca gatcgtggag ttcaacgcga cgtcggcgac ggcgcccgcc
      841 cggaccctcg gcgggccgct ccaggtcacc gctgccaccc cgggctccga ctcggtcgtg
      901 ccgatatctg cgccgaccca gaacgccgac gtggccggtt cggtcgagag ctcctccggc
      961 tcgggttcca tggcctacac gggcaccgag cccgtcctgc tcatcgtggt gggagcatcg
     1021 atcctgatcc tgggggcggt cctgctcctc ctgcagcgcc gccggccgat cgacagctga
     1081 ggacggcgtg gccgaccggg gtcgcgccga gcccggccga tcgggggatg cgcgagagcc
     1141 gacggttcac cccgacgcga tcgtctggac cggttcggcc cgcgatccgg gacagccgtt
     1201 ctctcctgcc accttcaggc tggatgccga ggcgattgcc atcacgatcc accgcggcgt
     1261 cggcagcggc acggtcgtcg tcccgctcgc cgacgtggtc ggactgagcg cggtcgatcg
     1321 agacgacggt ctgcccgagc tggagctcga gacaggcgac ggggcggtgg gtcggctccg
     1381 atggtccgac gagtacaccg agccgttcgt cgtcgcgctc gagcgcagcg gcgacgggcg
     1441 acctgacgcc ggctcggagc gtgccgacgt ccgccacggg acatcgggaa cggacgcgag
     1501 ggaccgtgtc ctccggtccg ccgcgatgct gctcctcgtg tcgggcctgc tgatcggcct
     1561 gctcggggtg tacgtcgccg tcctcgactc ccgggtgcat cgctggcccg tcgaccgccc
     1621 agaggtcgac gacggacgcg agacgtggct gctgctcggc tccgactccc gcgccgcagc
     1681 gggctcgatg ccggacaggt cggtgttcgg gtcgacgtcc gaggtccccg gcgagcgggc
     1741 cgacgtcgtg ctcctggtgc aggaggggga cgggcaacgc tcgccgcggc tggtgttgct
     1801 accccgagat ctgctcgtgt tccgggccgg tcatggcgtc gaccggctgg cgctcaccct
     1861 gctcgacggg ccgggggtca cagcgacgag cgtctgccgt tcactgggag ttccggtcga
     1921 tcacgtcgtc acggtccgct tcgacgggat ccaacggttg gtcgactccg tgggcgggat
     1981 cgacgtccgt tccgagctcg agacccgaga tcccaacacc ggtctgcacc tgcggccggg
     2041 ccggaaccac ctcaacggtg cgggcgcggt cgcctgggcg cgctcgcgtc atcccgagag
     2101 gctggtgagc ggtgagtggc tgcccgacct cgacggggaa tcggtccggc aatcgtccca
     2161 acgtgagctc ctgtcgcagc tggcttcggc gatggcccac agggccggac gcgaccccgt
     2221 caccctccag cgcctcatct ggacgtcagc cggttcggtc gagaccgaca gcggcaccgg
     2281 cccgctcgac ctggcgcgtc tggccgtggc gctcctcgga gccgaagacg ggggatcggt
     2341 cgcccacgtg gtgtcggatg cgcccatccc ggtggcccga ttgcttccgg aagcccatcg
     2401 tcagctcgat cggttccgcg gcaatcggga ctcgacgaca ccgtgtccgc gtgcccgcat
     2461 cagcgtcgac ggtcagcgat gagcgccggt cggtgccgac agcgggctgt ccgtagcctg
     2521 aggtcatggc gcggccgcaa cccctgaccg gcggcgactt ggacgcggcg ctggtcgggc
     2581 tacccggctg gaccgtcgtc gacggacggc tgcaccggga gctgcgcttc gccgacttcc
     2641 cccgcgcctt cgggttcatg gccgcggtgg cgaccgtcgc acaggcgatg gaccatcacc
     2701 cggactggac gaactcgtac gcgtcggtca ccgtcgatct gatgacccat gacgtcggcg
     2761 ggatcacgtc gctcgacatc cagctggcgc agcgcatctc ggatctcgcc ggcgaccagc
     2821 ggccctcctg atcggccgcg tcccggtcgc actccgaccg gtcgggaccg gtctcaggct
     2881 tcgtcgatcg cctgcagcag cgtgttccaa ctgagcgtcg cgcacttgat gcggacgggg
     2941 aacttcacga cgccgcgcag cgcctcgagg tcgccgaggc gtgactcgcc cg
//
LOCUS       DAOQOD010000272         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_17496_length_2979_cov_9.552326, whole genome shotgun sequence.
ACCESSION   DAOQOD010000272 DAOQOD010000000
VERSION     DAOQOD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_2979_cov_9.552326"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLZ72_15715"
     CDS             complement(<1..386)
                     /locus_tag="PLZ72_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37797.1"
                     /translation="MLVELAVRDLGVIAEARIPLPPGLTALTGETGAGKTMVVEALGL
                     LCGARPDPSRVRPGADAAVVEGLFADGDGSEGDGSEWVLRRVVPSSGRSRAYVDGGLS
                     TAADMAELGGRSIEIHGQHAQQALLAP"
     gene            complement(446..1291)
                     /locus_tag="PLZ72_15720"
     CDS             complement(446..1291)
                     /locus_tag="PLZ72_15720"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNA37798.1"
                     /translation="MSTFGLVLHRERAGDLARSTVNWLLERGHEVRVLAEDAGVLDDA
                     ELSTRAEDLCAGTDLVLSLGGDGTMLRAVELAAADDVPVLGVNLGQLGYLTEVEPAGV
                     RMALKRFLAGSYSLEERMRVEAEIADADGVRTSSALNEVVVEKSEMGHTVRLDVALDG
                     RPFTSYVADGLILATPTGSTAYVFSVRGPIVDPRHRAVLMAPVAAHMLFDRSIVLRPD
                     CEVRVTIGGDRAAGVSVDGRPFARLEPGSTVRCRASDQPARLVTFGERDFHAVLREKF
                     QLGPR"
     gene            complement(1288..2112)
                     /locus_tag="PLZ72_15725"
     CDS             complement(1288..2112)
                     /locus_tag="PLZ72_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNA37799.1"
                     /translation="MRRRLDAELVRRGLAPSRSAAARLIGEGQVIVSGALAERPARMV
                     DPSEPVRLAGPPPRFVGRGGEKLAAALERFGLDPSGRRALDAGASTGGFTDCLLQNGV
                     MSVTALDVGHGQLHERLRADPRVEVVERTNLRHVDPAATDPYGIVVADLSFISLNTVM
                     GVLVSSTAVGGDLVVLVKPQFEAGRRDVARGRGVITDPQIWTRTVTDVVATAVAAGAA
                     MMDVMVSPIHGADGNTEFLVHLRRGGPADRRSGEDRTAELIGAAVADAVARSGAGS"
     gene            complement(2188..>2979)
                     /locus_tag="PLZ72_15730"
     CDS             complement(2188..>2979)
                     /locus_tag="PLZ72_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNA37800.1"
                     /translation="PVGLWVIDLDGVVWRGPEAVPGSPDAVASLLERGDRVVFCTNDA
                     AGPADKAEHLRRLGVPACPVITSADAVVAACADAAAVLVLGRPGLVRYLGDSGLPVTD
                     VASLPDGGSLDGVDVVVVGALGDWDRSRIGMAADAVRSGARFLAANDDATFPTAGPDG
                     PRLLPGNGALVAAVATAAGRSPEIAGKPHAPMASVIVERHGPVDVVVGDKPETDGALA
                     VALGARYGLVLSGVTSAADLPADPAPWAVGADLADLVGASGAGRP"
BASE COUNT          413 a         1105 c         1113 g          348 t
ORIGIN      
        1 ggagccagca gcgcctgctg ggcgtgctgg ccgtggatct cgatcgaccg gccgcccagc
       61 tcggccatgt cagccgcggt gctgaggccg ccgtcgacgt atgcccgcga ccgtcccgag
      121 gacggcacga cccgccggag cacccattcc gagccgtcgc cttcggaacc gtcgccgtcg
      181 gcgaacaggc cctcgaccac cgcggcgtcg gcccccggtc gaacccgcga cggatcgggt
      241 cgcgcgccgc agagcaggcc gagggcctcg accaccatgg tcttgcccgc gccggtctcg
      301 cccgtgagcg cggtcagacc ggggggcagg gggatccggg cctcggcgat caccccgagg
      361 tcccggacgg cgagttcgac gagcatgacg gcccggagcg tagaggaccg ccgctgtcgg
      421 accgcgccgg gccgttccgg gccgctcagc gagggccgag ctggaacttc tcgcgcagca
      481 cggcgtggaa gtcccgttcg ccgaaggtga ccaggcgcgc gggctggtcg ctcgcccggc
      541 accgcaccgt cgaaccgggt tcgagtcggg cgaaggggcg tccgtccacc gacactcccg
      601 ccgcccggtc accgccgatg gtgactcgca cctcgcagtc gggccgcagc acgatcgagc
      661 ggtcgaacag catgtgggcc gccaccggcg ccatgaggac ggcccggtgg cgggggtcga
      721 cgatcgggcc gcggaccgag aacacgtagg cggtcgagcc ggtcggggtg gccaggatca
      781 gaccgtcggc cacataggag gtgaacggtc ggccgtcgag cgccacgtcg aggcgcaccg
      841 tgtggcccat ctcggacttc tcgaccacga cctcgttgag ggcgctcgag gtgcggaccc
      901 cgtccgcatc tgcgatctcg gcctcgaccc gcatccgctc ctcgagcgag taggagccgg
      961 ccaggaaccg cttgagcgcc atgcggacac ccgcaggctc cacttcggtc aggtagccga
     1021 gctggcccag gttgactccg aggacgggca cgtcgtcggc cgcggccagc tcgacggcgc
     1081 gcagcatggt gccgtcaccg cccaggctga gcaccaggtc ggtgcccgcg cagaggtcct
     1141 cggcccgggt cgacagctcc gcgtcgtcga gcacgccggc gtcctcggcc aggacgcgca
     1201 cctcgtgacc acgctccagc agccagttca ccgtcgagcg ggcgaggtcc ccggcccgct
     1261 cacggtgcag gaccagcccg aaggtgctca cgacccggct ccggagcggg cgaccgcgtc
     1321 ggcgaccgcg gcaccgatca gctccgcggt ccggtcctcc ccggaacggc ggtcggcggg
     1381 gccaccgcgg cgcaggtgga ccaggaactc ggtgttgccg tcggctccgt ggatcggcga
     1441 gaccatcacg tccatcatgg ccgcgccggc cgccacggcc gtggccacga cgtcggtgac
     1501 ggtgcgggtc cagatctgtg ggtcggtgat cactccgcgg ccccgggcga cgtcgcgacg
     1561 accggcctcg aactggggct tcaccagcac caccaggtcc ccgccgaccg cggtcgagga
     1621 gaccagcacg cccatgaccg tgttgagcga gatgaacgac aggtcggcca cgacgatccc
     1681 gtaggggtcg gtggcggcag ggtcgacgtg ccggaggttg gtccgctcga ccacctcgac
     1741 gcggggatcg gcccggagcc gttcgtgcag ctggccgtga cccacgtcga gcgcggtgac
     1801 cgacatgacg ccgttctgga gcaggcagtc ggtgaagccg ccggtggatg cgccggcatc
     1861 gagcgcccga cgtcccgacg ggtcgagccc gaatcgctcg agcgcggcgg cgagcttctc
     1921 gccgccgcgg cccacgaagc gcgggggcgg gccggcgagt cgtacgggtt cggacgggtc
     1981 gaccatgcgg gccgggcgct ccgccagggc gccggacacg atcacctgtc cctcgccgat
     2041 cagtcgggcc gccgccgagc gcgacggggc cagtccacgg cggaccagtt cggcgtccag
     2101 tcgtcggcgc accgtccgag gctaccgagc gccgcttccg ggtccggccg ctcgtggttc
     2161 cggccgttcg tggttctggc tgatccgtca gggtcggccc gcgccggagg ctccgaccag
     2221 atccgccaga tccgcgccga ccgcccacgg ggcggggtcg gcgggcaggt cggcggcgga
     2281 ggtcaccccg ctgagcacca ggccgtatcg ggccccgagg gcaaccgcga gggcgccgtc
     2341 ggtctcgggc ttgtccccca cgacgacgtc gaccgggccg tggcgctcga cgatcaccga
     2401 cgccatcggg gcgtgcggct tgccggcgat ctccgggctg cgtcccgccg cggtcgccac
     2461 cgccgcgacc agagcgccgt tgccgggcag cagccgcgga ccgtcgggac cggcggtcgg
     2521 gaaggtggcg tcgtcgttgg cggccaggaa ccgcgccccg gagcgcacgg cgtcggcggc
     2581 catgccgatg cgggagcggt cccagtcccc cagcgcgccg accaccacga cgtcgacccc
     2641 gtcgagggac ccgccgtcgg gcagggaggc cacgtcggtc accgggaggc cgctgtcgcc
     2701 caggtaccgg accagaccgg gccgaccgag caccaggacc gccgcggcat ccgcgcatgc
     2761 ggccacgacg gcgtcggccg aggtgatcac gggacaggcc gggacgccga gccggcgcaa
     2821 gtgctcggcc ttgtcggcgg gaccggcggc gtcgttggtg cagaacacga cccggtcgcc
     2881 ccgctcgagc agggacgcga cggcatcggg agagccgggg accgcctcgg gtccgcgcca
     2941 caccacaccg tcgaggtcga tcacccacag gcccacggg
//
LOCUS       DAOQOD010000273         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_17840_length_2949_cov_10.164824, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000273 DAOQOD010000000
VERSION     DAOQOD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17840_length_2949_cov_10.164824"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1280)
                     /locus_tag="PLZ72_15735"
     CDS             complement(75..1280)
                     /locus_tag="PLZ72_15735"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNA37801.1"
                     /translation="MIPLADALEHVLARVPAQPTVEVPLAEAHGCVATDDVVSSETIP
                     AWDNTALDGYAVRAADVCVPGAVLDVVTTIAAGSAGEDPVGAGQAARIMTGAPMPPGA
                     DAVVMVERTSMLDDGRRVLVDAAVAEGTGVRRVGDDVRAGDVVVAAGTVLRAGHLGLL
                     ASVGAVSVRVRRPVRVGVLSTGDELVEGGAPLRRGQIRDSNRLALLALGRSSGFEMID
                     LGLVPDDEEAIEAAIRAGCADADALLTSGGVSMGDFDHVKVVLGRIAEMRWMQIAIKP
                     AKPFAFGLLDGVPVFGLPGNPVSSLVSFELLARPALRRAAGHVDIHRRRVRAVAEEDL
                     RRRPDGKTHFVRVTLSQDDRLDFRVRSAGGQGSHQMTALAAADGLAVLPDGDGVAAGA
                     DVEVILLDG"
     gene            complement(1354..2235)
                     /gene="galU"
                     /locus_tag="PLZ72_15740"
     CDS             complement(1354..2235)
                     /gene="galU"
                     /locus_tag="PLZ72_15740"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNA37802.1"
                     /translation="MSHVTKAVIPAAGLGTRFLPATKAQPKEMLPVVDKPAIQYVVEE
                     AVAAGIDDILIITGRNKRSLEDHFDRNFELEYQLQSKGKTGDLAEVVELAELADIHFV
                     RQGEPLGLGHAVSVARKHVGNEPFVVMLGDDIMDDHSTVLPDMIKQFGETGSSIVALM
                     EVPPESISAYGCAAVDDREGSLCRITGIVEKPAVEDAPSNLAVMGRYLFTPEIFAELE
                     HVEPGVGGEIQLTDAIAGLLGHDDVYGWTFCDGRYDIGKKIDYLRATVELALQREDLG
                     PEFRQFLAEVVRREGIV"
     gene            2480..2836
                     /locus_tag="PLZ72_15745"
     CDS             2480..2836
                     /locus_tag="PLZ72_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNA37803.1"
                     /translation="MPVYEYRCRDCGHEFEIQQSMSDDTLTVCPSCGGDLRKVFSPVG
                     IAFKGSGFYKNDSGSRSGSSKGDGSKADGSKSESSASGSDSSSSSSSSSSSSTSSSKG
                     DGSGSSAPATSGSTSS"
BASE COUNT          434 a         1074 c         1007 g          434 t
ORIGIN      
        1 cgttctgtag tgcgagatcc aggcgttcgc ctggatctcg cactacagaa cggggctcag
       61 cggtgagaac gggttcagcc gtcgaggagg atgacctcga cgtcggcgcc ggccgcgacc
      121 ccgtcgccgt cggggaggac ggccagaccg tccgccgcgg ccagggcggt catctggtgc
      181 gacccctgtc ccccggcgga gcgcacacgg aaatcgagtc ggtcgtcctg ggacagcgtc
      241 acccgcacga aatgggtctt gccgtcgggg cgccgtcgca ggtcctcctc ggccaccgcc
      301 cggacccgac gtcggtggat gtcgacatgt cccgcggcgc ggcgcagcgc ggggcgggcc
      361 agcagctcga agctgaccag cgagctgacc gggttgcccg gcagcccgaa caccggcacc
      421 ccgtcgagca gtccgaacgc gaacggcttg gccggcttga tggcgatctg catccagcgc
      481 atctcggcga tgcggccgag cacgaccttg acgtggtcga agtcccccat cgacacgccg
      541 ccgctggtca ggagcgcgtc cgcgtcggcg caccccgccc ggatggccgc ctcgatcgcc
      601 tcctcgtcgt cgggcaccag tcccaggtcg atcatctcga agcccgagga ccgaccgagc
      661 gccagcagcg ccagccggtt cgagtcgcgg atctgacccc gtcgcagcgg agccccacct
      721 tcgaccagct cgtcgccggt cgacaggacc ccgacgcgca ccggtcgacg cacccggacc
      781 gacaccgcgc cgaccgacgc cagcaggccc aggtgcccgg cccgcaggac cgtcccggcg
      841 gccacgacca cgtcgccggc gcgcacatcg tcgccgaccc gccgcacccc ggtcccctcg
      901 gccaccgccg cgtcgacgag cacgcgccgg ccgtcgtcga gcatcgaggt ccgctcgacc
      961 atcacgacgg cgtcggcccc tggcggcatg ggcgcaccgg tcatgatgcg cgccgcctgg
     1021 cccgcgccca cagggtcctc acccgcgcta cctgccgcga tggtggtgac cacgtcgagc
     1081 accgctccgg gaacgcagac gtccgcggcg cgcacggcgt acccgtcgag cgcggtgttg
     1141 tcccaggcgg ggatcgtctc gctcgacacg acgtcgtcgg tggcgacgca gccgtgggcc
     1201 tccgcgagcg ggacctcgac ggtcggctgg gcgggcaccc gggccaggac gtgttcgagc
     1261 gcgtcggcca gcgggatcac ggcttcagac gataccggcc gcccggaacg gccgatccct
     1321 cacggccaac ccgtcaccgg tcacgccgcc gggtcagacg atgccctcgc ggcggaccac
     1381 ctcggccagg aactggcgga actccggacc caggtcctct cgctgcagcg ccagctccac
     1441 cgtggcccgc aggtagtcga tcttcttgcc gatgtcgtag cggccgtcgc agaacgtcca
     1501 tccgtagacg tcgtcgtgac cgaggagtcc ggcgatggcg tcggtgagct ggatctcgcc
     1561 gccgacgccc ggctcgacgt gctcgagctc ggcgaagatc tccggcgtga acaggtaccg
     1621 acccatcacc gccaggttcg acggggcgtc ctcgaccgcc ggcttctcca cgatgccggt
     1681 gatgcggcac agcgacccct cgcggtcgtc gaccgcggcg cagccgtagg cggagatgct
     1741 ctcgggcggg acctccatca gcgccacgat cgaggagccg gtctcgccga actgcttgat
     1801 catgtcgggc agcaccgtcg agtggtcgtc catgatgtcg tcgccgagca tgaccacgaa
     1861 cggctcgttg ccgacgtgct tgcgggcgac cgacacggcg tggcccaacc cgagcggttc
     1921 gccctggcgg acgaagtgga tgtcggccag ctcggccagc tcgaccactt cggccaggtc
     1981 gccggtcttg cccttggact gcagctggta ctcgagctcg aagttgcggt cgaagtggtc
     2041 ctcgaggctg cgcttgttgc ggccggtgat gatcaggatg tcgtcgatgc ccgccgcgac
     2101 cgcctcttcc accacgtact ggatggcggg cttgtcgacg accggcagca tctccttggg
     2161 ctgggccttg gtggcgggca ggaaccgggt ccccaggccc gcggccggga tgacggcctt
     2221 ggtgacgtga ctcatgcgac gttccccatc tgcagttgcc cctcacgtcg gtgcacgtgg
     2281 cacttccgat tcgtgttgtc cgctcctcga tccgaccgtg ccggaccgca ctgatcgtaa
     2341 tgcccgggga gcggaatccc ctcccggcag atgtcccagc gcccggcatg ggccgttcga
     2401 cccaggcgac ccggacgctg ccgaacgcag ccgggtggct tggggaccgg cgcgggtgct
     2461 gcggtacgat cccgccacca tgcccgtcta cgagtaccga tgccgcgact gcggccacga
     2521 gttcgagatc cagcagtcga tgagcgacga caccctcacc gtgtgcccgt cctgcggcgg
     2581 cgacctccgc aaggtcttct caccggtcgg gatcgcgttc aaggggtcgg gcttctacaa
     2641 gaacgactcc gggtcgcggt cgggttcgtc caagggcgac ggatccaagg ccgacgggtc
     2701 caaatccgag tcgtcggcct cgggcagcga ttccagctcg agctcgtcga gctcgtcgag
     2761 ctcgtcgacg agcagctcga agggcgacgg ctcgggatcg agcgctccgg ccaccagcgg
     2821 ctccacgtcg agctgacccg ttctgtagtg cgagatccag gcgttcgcct ggatctcgca
     2881 ctacagaacg ggaatgggcg ggggctcaga cccgctgggt ggcgcggtgg tccgacatgg
     2941 cccgctgca
//
LOCUS       DAOQOD010000274         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_18003_length_2935_cov_12.001389, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000274 DAOQOD010000000
VERSION     DAOQOD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18003_length_2935_cov_12.001389"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLZ72_15750"
     CDS             complement(<1..832)
                     /locus_tag="PLZ72_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37804.1"
                     /translation="MQHLSHTSVDAPRTHRGLPSLLVAALAAISLAVALVPATPAAAQ
                     EDGGSSLAELQKKRDAVRAQKAAKASEVNGLKATDAQVSAALSALDAQVNAQQDRVEE
                     AQRAVSQAESERSAAESAQARTQQELDDLRARMKTSAVEAYVTMGSSDPMSGVGVDDV
                     NDAVNKRTLLSVQANENTDVVEQFRSKQEDLELQRAAATAAAERAARSKAAVQDRLGD
                     LNAAFEKQQAFAAQVDSRLDRALSEADSLASIDSNLSSTISAKQAEIARALAAQRAAA
                     E"
     gene            complement(1014..1844)
                     /gene="mutM"
                     /locus_tag="PLZ72_15755"
     CDS             complement(1014..1844)
                     /gene="mutM"
                     /locus_tag="PLZ72_15755"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNA37805.1"
                     /translation="MPELPEVETIRRQLVDRLTDRQILDAWGFNSPKFSAAPAAAGTT
                     VTDVTRRGKYLLLPLRRDDAPPTQELIVHLGMTGRLAVTDRSIDRIEGEPHLRAWWCL
                     GDGSVLTFHDVRRFGRIAVVDLGDHRSLPTLAALGPEPDSEEFTAEHLRRRVNAGSRR
                     IKTVLLSQRVVAGVGNIYADEALWRAGVDPRARHLTRAAAARLRDAIRDVLSEGVDNG
                     GTTLRDYVDADGSPGRNQHRLDCYGRAGRPCHRCGTELRGTVVDARSTSWCPDCQRRR
                     "
     gene            1966..2646
                     /locus_tag="PLZ72_15760"
     CDS             1966..2646
                     /locus_tag="PLZ72_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNA37806.1"
                     /translation="MPNADDHRRSDDRTTIDLRADSDTRDRLVQAAADVFREKGYPGS
                     RVQEIARRAGFTSGALYAHFDSRAELLAEAIAVENSRMFELVSDALGRSIVDGSGAIA
                     SSIADFVKAETAPTDQLMLDGFAICTREPEAQVRIGESLNHLLDQLEEKIGRMAVEPG
                     SLMDDDPGGMAYLMVSFLCGVAALRAAGLSDRAPHDAENVLAMFLHGLGALDDPAAAT
                     TAAEGGPG"
     gene            2672..>2935
                     /locus_tag="PLZ72_15765"
     CDS             2672..>2935
                     /locus_tag="PLZ72_15765"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNA37807.1"
                     /translation="MGILAVVNQKGGVGKTTVALGLASAAMMRGRRALVVDMDPQANA
                     TTGLGVFDATTTIDEVLAEDRPGAASAAVRRSGWEPAVGHAPDL"
BASE COUNT          380 a         1006 c         1113 g          436 t
ORIGIN      
        1 cctcggcggc ggcgcgctgg gcggccagcg cccgggcgat ctcagcctgc ttggccgaga
       61 tggtcgacga caggttggag tcgatggacg cgagcgagtc ggcctcggac agggcccggt
      121 cgagtcggga gtcgacctgc gcggcgaacg cctgctgctt ctcgaaggcg gcgttgaggt
      181 cgcccaggcg atcctgcacc gcagccttgc tgcgggcggc ccgctcggcc gccgcggtgg
      241 ccgccgcacg ctggagctcg aggtcctcct gcttggagcg gaactgctcg accacgtcgg
      301 tgttctcgtt ggcctggacc gacagcaggg tgcgcttgtt gaccgcgtcg ttgacgtcgt
      361 cgacgcccac gcccgacatc gggtcggagc tgcccatggt gacgtaggcc tcgaccgccg
      421 aggtcttcat gcgggcccgc aggtcgtcga gctcctgctg ggtccgggcc tgagcggact
      481 cggccgcaga tcgctcggac tcggcctggc tgacagctcg ctgggcctcc tcgaccctgt
      541 cctgctgtgc gttcacctgg gcgtcgaggg cgctcagcgc cgctgacacc tgggcatcgg
      601 tcgccttgag gccgttgacc tcggacgcct tggccgcctt ctgggcacgg acggcgtccc
      661 gcttcttctg gagctcggcc aggctcgatc cgccgtcctc ctgcgccgcg gcgggggtgg
      721 cggggaccag cgccaccgcg agggagatgg cggcgagggc ggcgaccagc aggctgggaa
      781 ggccgcggtg cgtccggggg gcgtcaacgg aggtgtggga gaggtgctgc acgtggtcct
      841 gggagggatc ggtcggcgcc ggggcgaggg ggcgccgatt cgttcgtaac gagaatgcaa
      901 tctaacacac ccttcccgcc catccagtcg gtgcgcatga ggcccctccc agctccgtcc
      961 gccgacgggg ctggacccat gcgggccggg tcggccgcgg cgctcgggga cggtcatcga
     1021 cgtcgctggc agtcggggca ccaacttgtc gatcgggcat cgacgaccgt accccgcagc
     1081 tcggtgccgc aacggtggca gggccggccg gcccgtccgt agcagtcgag gcgatgctgg
     1141 ttgcgtccgg gcgaaccgtc ggcgtcgacg tagtcgcgaa gggtcgtgcc tccgttgtcg
     1201 actccttcgg acagcacgtc gcggatggcg tcgcgcagac gggcggccgc ggcgcgggtc
     1261 aggtgtcggg cgcggggatc gaccccggcg cgccacagcg cctcgtccgc gtagatgttg
     1321 ccgacgccgg cgaccacgcg ctgggacagc aggacggtct tgatccgacg tgaccccgcg
     1381 ttcacccgcc gccgcaggtg ctcggcggtg aactcctcgg agtcgggttc ggggccgagt
     1441 gcggccaggg tcggcagcga ccggtggtcg ccgaggtcga cgacggcgat gcgaccgaag
     1501 cgacggacgt cgtggaaggt cagcaccgaa ccgtcgccga ggcaccacca cgcccgcagg
     1561 tgcggctcgc cctcgatgcg gtcgatcgac cggtcggtca ccgcgagccg gcccgtcatc
     1621 cccaggtgga cgatcagctc ctgtgtgggc ggcgcgtcgt cgcggcggag ggggagcagc
     1681 aggtacttgc ccctccgggt cacgtcggtg accgtcgtgc cggccgcggc gggcgcggcg
     1741 gagaacttcg gggagttgaa gccccacgcg tcgaggatct ggcggtcggt cagccggtcg
     1801 accagctgac gtcggatcgt ctccacctcc ggcagctcgg gcactgcccg agtgtgcccc
     1861 gctgcggtcc ggcggaccat ccgccggtct ctgggggtcg cggtccggtc gccgggtccg
     1921 cgagtagtac cgtcacggac ggatcaggag tcacggaggg gccggttgcc gaacgccgac
     1981 gatcaccgcc ggtcggacga ccgcacgacg atcgacctcc gcgcggactc cgacacgcgg
     2041 gaccgactgg tgcaggccgc ggcggacgtg ttccgcgaga agggataccc gggttcccgg
     2101 gtccaggaga tcgctcgacg ggcggggttc acctcgggtg ccctctacgc ccacttcgac
     2161 agccgggccg agttgctggc cgaggcgatc gccgtcgaga acagtcggat gttcgagctg
     2221 gtctcggacg cactgggtcg gtcgatcgtc gacggttccg gggcgatcgc ctcgtccatc
     2281 gccgacttcg tgaaggccga gacggctccc accgaccagt tgatgctgga cggcttcgcc
     2341 atctgcactc gcgaacccga ggcgcaggtg cggatcggcg agtccctcaa ccacctcctc
     2401 gaccagctgg aggagaagat cggtcggatg gcggtcgagc cgggcagcct gatggacgac
     2461 gaccccgggg gcatggccta cctgatggtc tcgttcctgt gcggggtggc ggcgctccgc
     2521 gccgccgggc tgagtgaccg tgccccccac gacgccgaga acgtcctggc gatgttcctc
     2581 cacggcctgg gagcgctcga cgacccggcc gccgccacga ccgccgctga gggtggcccg
     2641 ggctgagcgg ccgtcggagt accgttgccc catggggatc ctcgccgtgg tcaatcagaa
     2701 ggggggcgtc ggcaagacga ccgtcgccct cggcctcgcc tccgcggcga tgatgcgcgg
     2761 gcgtcgggcc ctggtggtcg acatggatcc ccaggccaac gccaccaccg ggctcggggt
     2821 gttcgacgcg acgaccacga tcgacgaggt cctcgccgag gaccggcccg gtgcagcctc
     2881 ggccgcggtg cgccgctcgg ggtgggagcc ggcggtgggc cacgctccgg acctg
//
LOCUS       DAOQOD010000275         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_18306_length_2907_cov_10.397616, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000275 DAOQOD010000000
VERSION     DAOQOD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_2907_cov_10.397616"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1764
                     /locus_tag="PLZ72_15770"
     CDS             139..1764
                     /locus_tag="PLZ72_15770"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNA37808.1"
                     /translation="MIENVGKWGLWSVLALGVVAGVLAVAVDPGWWWAELVLVPLVLL
                     GVWDLVQTRHSILRNFPILGHFRFLIEGVGSEMHQYLVESNTDGRPFNRDTRSLIYQR
                     AKKAADKKPFGTELDVYENGYTWLAHSITPRPMPEDPVGDLRVEVGGLQCARPYSLSV
                     LNVSAMSFGALSANAVRALNRGARAGRFAHDTGEGGISRYHREHGGDLIWQIGTGYFG
                     CRDDQGGFDADLFAATAADEQVKMIELKLSQGAKPGHGGILPAAKVTDEIAEARRVPV
                     GVDCLSPTSHRAFSTPREMVSFLDRLRDLSGGKPVGFKICIGDPREFSAIVKAMIEAD
                     SYPDFVVVDGGEGGTGAAPLEFSNSLGMPLVEGLMLVQNVLTGAGVRDRMRVGASGKM
                     VTASAIAQSMALGADWCNTARAFMMAVGCIQAQQCHTNRCPVGVATQDPKLQRALVVD
                     DKWVRVEQFHRNTVHALAEIVAAMGLDHPAQLEPDHVVRRVSQYEVRTFGEIHPTLPS
                     GALLEGVAPERFQRMWDSVGTDAFRPSTGRLMG"
     gene            complement(1930..>2907)
                     /locus_tag="PLZ72_15775"
     CDS             complement(1930..>2907)
                     /locus_tag="PLZ72_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNA37809.1"
                     /translation="PPVPGADTVLNRTAAPSTSPTDPSARPTPTPPTGSGRALAVGRR
                     PSLVLAVASVGGLVMFLWPLLVSPPDEVGHVTDAPFLFALILPVVIAVVLSELHSGGI
                     DTKALAMLGVLTAIGAALRPMGAGVAGLETVFFLLVLAGRVYGAGFGFVLGTTTMFTS
                     AILTGGIGPWLPFQMMASGWVGLGAGLLPDSIAGRALRGRTEIAVLALYGAVAAYAYG
                     FLMNMWFWPFAIGAGTELSFVAGAPVVENLHRFVLFTLATSTLGWDTGRAITNVVAVV
                     LLGPIVLTTLRRSVRRAAFDAPVAFDAPVSIDATVSIDATPAGPTPPNL"
BASE COUNT          444 a          959 c         1096 g          408 t
ORIGIN      
        1 ctaggggacg ccggtcgtat acgcccgctg tccgttcgga catgaccggt gcgggggcgg
       61 atggtccccg tagtctcggc gcccggtacc cggtgggccc ctcgggcgtc accggagacg
      121 atggtgaacg gggggaccgt gatcgagaac gtggggaagt gggggctgtg gtcggtgctc
      181 gcacttggcg tcgtggccgg cgtgctcgcg gtcgcggtcg acccggggtg gtggtgggcg
      241 gagctcgtcc tggtgccgct cgtcctgctc ggcgtctggg atctggtgca gacccgccac
      301 agcatcctgc ggaacttccc gatcctcggg cacttccggt tcctgatcga gggcgtcgga
      361 tccgagatgc accagtacct ggtcgagagc aacaccgacg gtcggccctt caaccgcgac
      421 acccggtccc tcatctacca gcgggccaag aaggcggccg acaagaagcc gttcggtacc
      481 gagctcgacg tctacgagaa cggatacacc tggctggcgc actcgatcac gccgcgcccg
      541 atgccggagg acccggtggg tgacctccgg gtcgaggtcg ggggactgca gtgcgctcgg
      601 ccctactcgc tgtcggtgct caacgtgtcg gccatgagct tcggggccct gagcgccaac
      661 gcggtccggg cgctgaaccg gggggcgcgg gcggggcgat tcgcccacga caccggcgag
      721 ggcggcatct cgcgctacca ccgcgagcac ggcggcgacc tgatctggca gatcggcacc
      781 ggctacttcg gctgccgcga cgaccagggc gggtttgacg ccgacctgtt cgccgcgacc
      841 gcggcggacg agcaggtgaa gatgatcgag ctcaagctct cccagggcgc caagcccggc
      901 cacggcggga tcctgcccgc ggccaaggtc actgacgaga tcgccgaggc ccgtcgggtg
      961 cccgtcggcg tcgactgcct gtcgcccacc agccaccgcg ccttctcgac ccccagggag
     1021 atggtgtcgt tcctcgatcg cctgcgcgac ctgtcgggcg gcaagccggt gggcttcaag
     1081 atctgcatcg gcgatccgcg tgagttctcc gcgatcgtca aggcgatgat cgaggcggac
     1141 tcgtatccgg acttcgtcgt cgtcgacggc ggggagggcg gaaccggcgc cgctccgctc
     1201 gagttctcca actcgctcgg catgccgctg gtcgagggtc tgatgctggt ccagaacgtg
     1261 ctgaccggcg ccggggtgcg ggaccggatg cgggtggggg ccagcggcaa gatggtcacc
     1321 gcgtcggcca tcgcccagtc gatggcgctg ggcgcggact ggtgcaacac cgctcgggcg
     1381 ttcatgatgg cggtcggttg catccaggcc cagcagtgcc acaccaaccg gtgtccggtg
     1441 ggcgtcgcca cccaggaccc gaagttgcag cgggcgctgg tcgtcgacga caagtgggtg
     1501 cgcgtcgagc agttccaccg caacacggtc cacgccctgg ccgagatcgt cgccgccatg
     1561 gggctcgacc acccggcgca gctcgaaccg gaccacgtcg tgcgtagggt cagccagtac
     1621 gaggtgcgga ccttcggcga gatccatccg acgctgccgt cgggggcgtt gctggagggg
     1681 gtcgcgccgg agcgcttcca gaggatgtgg gacagcgtcg gcaccgacgc cttccgcccc
     1741 tcgaccggcc gcctcatggg ctgagccagc ccgaacttgt agctgggggt cgctggggtg
     1801 gcgtgggggc tacaagttcg gcggcggctg gcgttgttgt agctggtggt cgctggggcg
     1861 acttgtgggc tacaagttcg gcggcggctg gcgatgttgt agctgggggt cgctggggtg
     1921 gcgtgtgggc tacaagttcg gcggggtcgg gccggccggc gtcgcgtcga tggagacagt
     1981 cgcgtcgatg gagaccgggg cgtcgaaggc gaccggggcg tcgaaggcgg cgcggcgcac
     2041 cgatcgacgc aacgtcgtca acacgatcgg tccgagcagc acgaccgcga cgacgttggt
     2101 gatcgcccga ccggtgtccc agccgagggt cgaggtcgcc agggtgaaca gcacgaaccg
     2161 gtgcaggttc tcgaccaccg gggccccggc cacgaacgac agctcggtcc cggcaccgat
     2221 ggcgaacggc cagaaccaca tgttcatcag gaacccgtac gcgtaggcgg cgacggcccc
     2281 gtagagcgcc aggaccgcga tctcggtccg accgcgcagc gcccgaccgg cgatcgagtc
     2341 gggcagcaac ccggcgccga ggccgaccca gcccgaggcc atcatctgga acgggagcca
     2401 cggcccgatg ccgccggtca ggatcgccga ggtgaacatg gtggtggtgc cgagcacgaa
     2461 gccgaacccg gcgccgtaga ccctcccggc gagcaccagc aggaagaaca cggtctccag
     2521 gccggccaca ccggcgccca tcgggcggag cgcggcgccg atggcggtga gcacgccgag
     2581 catggccagc gccttggtgt cgatgccccc ggagtgcagc tcggacagga cgacggcgat
     2641 gacgaccggg aggatgagcg cgaacaggaa cggggcgtcg gtcacgtggc cgacctcgtc
     2701 gggcggcgac acgagcaacg gccacaggaa catgaccaga ccaccgaccg acgccaccgc
     2761 cagcacgagc gacggtcgcc gcccgaccgc caacgcccgg ccgctgccgg tcggtggtgt
     2821 gggcgtggga cgggccgacg ggtcggtcgg cgaggtgctc ggcgcggcgg ttcggttcag
     2881 cacggtgtca gctccaggga ccggcgg
//
LOCUS       DAOQOD010000276         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_19254_length_2832_cov_10.293842, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000276 DAOQOD010000000
VERSION     DAOQOD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19254_length_2832_cov_10.293842"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1267
                     /gene="cysS"
                     /locus_tag="PLZ72_15780"
     CDS             125..1267
                     /gene="cysS"
                     /locus_tag="PLZ72_15780"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNA37810.1"
                     /translation="MRLYDTAREAIVPFEPVDRVVTMYTCGITPYDSTHLGHAATYLT
                     YDVLQRRLRDLGHETRCVRNITDVDDSILPKARELGVHYLDLAAAELARFDADMERLE
                     MLPAWSEPRATSAIPDIRGFIGMVLDQGFAYQAGGAVYFEVANFPKFGQISHYSRDEM
                     LAFAAERGGNVEDPHKRDPLDFVLWQPSLPDEPAWESLWGPGRPGWHIECSALCLREL
                     GTTIDLHGGGSDLIFPHHECEAAQSEAATGEPLVRHWMHQAMVHMDGEKMSKSLGNLV
                     FVSDLSQSWDPRAIRLACVSHHYRSAWEWTDELMPAASERLSRWVAAGEGSGALEQVR
                     AALDSDLDTPGAIEAIDDAAASGLGVSEAAALLGVRTDRTVFESAT"
     gene            1392..2363
                     /locus_tag="PLZ72_15785"
     CDS             1392..2363
                     /locus_tag="PLZ72_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNA37811.1"
                     /translation="MAAENRKRDAGQMQSVFRAVLTPPFRFLWSVTTEGMDNIPVTGG
                     AIIAPNHISVLDSFFVPLVLPRRITYVGKAEYMDDWKTKFLFPAMGMIPIDRSGGDSA
                     TAALDAAAGCLEAGELFGIYPEGTRARDGLLHKGHTGVARLALRTGSPIVPVGIIGSD
                     QVQPPDAKFPAPFRRVHIRFGRPISVERYQDRANDRMVLRQIVDEVMYEIRNLSGQTY
                     VDSYATKPSKPVAAPAEPAVPTAPAAETDDRPVAPVIPIDRPHGRPVEPAAASTDRSR
                     PTGLVVASAAAGPTQVAVDSDGVDRRSSAAVIRSRPLDLDALALTGA"
     gene            2360..>2832
                     /locus_tag="PLZ72_15790"
     CDS             2360..>2832
                     /locus_tag="PLZ72_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37812.1"
                     /translation="MTSGPGRFRVSVILGAAVAVALALAACGSDRAADGDGSASATTV
                     TTPADPAARDAVRAGASPVIDLPAPPIDGMPVFVGNHGDDLVVVGGREFTRSGMERPS
                     DDVAVLDAGTFSWRSYPRPPFDHPTGYPIAAVTGDVVLISGFSCEIGTETHDSSGP"
BASE COUNT          396 a         1050 c          954 g          432 t
ORIGIN      
        1 ttcggtgggc gctgtcgttg ttgtagctct ggggtcgtcg ggtcgtcggt gggctacaag
       61 ttcggtgggc gccggtgggt gccgggtcgg gcgttcgacc gggcgcgggt tagcctgcgc
      121 caccgtgcgc ctgtacgaca ccgcccgaga ggcgatcgtc cccttcgagc cggtcgaccg
      181 tgtcgtcacc atgtacacgt gtggcatcac gccgtacgac tcgacgcacc tcgggcacgc
      241 ggccacgtac ctgacctacg acgttctcca gcggcggctc cgcgacctcg gccacgagac
      301 gcgctgcgtg cgcaacatca ccgacgtcga cgacagcatc ctgcccaagg cccgcgagct
      361 cggcgtccac tacctggacc tggccgccgc cgagctggcc cgcttcgacg ccgacatgga
      421 gcgcctcgag atgctcccgg cctggtccga gccgcgggcg acctccgcca tccccgacat
      481 ccgcggcttc atcggcatgg tgctcgacca gggtttcgcg taccaggcgg gcggagccgt
      541 gtacttcgag gtcgccaact tcccgaagtt cggacagatc agccactact cgcgtgacga
      601 gatgctggcc ttcgcggccg agcgcggcgg caacgtcgag gaccctcaca agcgggaccc
      661 gctcgacttc gtgctgtggc agccgtcgct tcccgacgag ccggcgtggg agtcgctgtg
      721 gggtcccggc cgtccgggct ggcacatcga gtgctcggcg ctgtgcctgc gcgagctcgg
      781 cacgaccatc gacctgcacg gcgggggcag cgacctgatc ttcccgcacc acgagtgcga
      841 ggccgcccag tccgaggccg ccaccggcga accgctggtg cgccactgga tgcaccaggc
      901 catggtccac atggacggcg agaagatgtc gaagtcgctc ggcaacctcg tgttcgtgtc
      961 ggacctgtcg cagagctggg acccacgggc catccgcctc gcctgtgtgt cgcaccacta
     1021 ccgcagcgcc tgggagtgga ccgacgagct gatgccggcc gcgtccgaac gcctgtcccg
     1081 atgggtcgcg gcgggcgagg gctccggggc gctcgagcag gtgcgcgccg ctctcgactc
     1141 cgacctcgac acccccggcg ccatcgaggc gatcgacgat gccgccgcat ccggcctcgg
     1201 cgtatcggag gccgcggccc tcctcggggt ccgcacggac cgaaccgtgt tcgagtccgc
     1261 gacctgaggg aatcccgcga aaaaggtcag gattcaggtc ttccggcccg gggcgtctgg
     1321 gtaggatcac tgcccagacg tcagcgagca ccgaccgccg ggccccgacg tcgtcgagga
     1381 ggggataccc cgtggccgcc gagaaccgca agcgagacgc cggacagatg cagtccgtgt
     1441 tccgcgccgt tctcaccccg ccgttccggt tcctctggag cgtgacgacc gaggggatgg
     1501 acaacatccc ggtcactggt ggggcgatca tcgcccccaa ccacatctcg gtgctcgact
     1561 cgttcttcgt gccgctcgtc ctgccccggc gaatcaccta cgtcggcaag gccgagtaca
     1621 tggacgactg gaagaccaag ttcctcttcc cggccatggg catgatcccg atcgaccgct
     1681 ccgggggtga ctcggccacc gccgcgctcg acgccgcggc cgggtgcctg gaggccggcg
     1741 agctgttcgg catctacccc gagggcaccc gggcccgcga cgggctgctg cacaagggcc
     1801 acacgggcgt cgcccgcctc gccctgcgga cggggtcgcc gatcgtgccg gtgggcatca
     1861 tcggctccga tcaggtgcag cccccggacg ccaagttccc cgcaccgttc cgtcgggtgc
     1921 acatccgctt cggtcgcccg atcagcgtgg agcgctacca ggaccgggcc aacgaccgca
     1981 tggtgctgcg ccagatcgtc gacgaggtca tgtacgagat ccgcaacctg tcgggtcaga
     2041 cctacgtcga cagctacgcc accaagccgt ccaagccggt cgccgctccg gccgaacccg
     2101 ccgtgccgac cgctccggcg gccgagaccg acgaccgtcc ggtcgccccg gtcatcccga
     2161 tcgaccgccc ccacggccga ccggtcgagc ccgcggctgc cagcaccgac aggtcccgac
     2221 ccacgggtct ggtcgtcgcg tcggccgctg cgggaccgac ccaggtcgcc gtcgactccg
     2281 acggcgtcga ccgccgttcg tcggccgcgg tcatccgatc ccgtccgttg gacctcgacg
     2341 ccctcgctct gaccggcgca tgacctcggg acccgggcgg ttccgggtgt cggtgattct
     2401 cggagcagca gtcgctgttg cgctggccct tgcggcgtgc gggtcggacc gcgccgccga
     2461 cggtgatggc agcgcgtccg ccacgacggt gaccaccccg gcggatcctg ctgcccgcga
     2521 cgcggtccgg gcgggtgcat ctccggtgat cgacctgcct gccccgccga tcgacgggat
     2581 gccggtgttc gtcggcaacc acggcgacga cctggtcgtc gtcggcggca gggagttcac
     2641 ccgctccggc atggagcgac cctccgacga cgtggccgtg ctcgatgccg ggacgttctc
     2701 gtggcgctcc taccctcggc ctccgttcga tcacccgacc ggctacccga tcgcggcggt
     2761 gaccggtgac gtcgtcctga tcagcgggtt ctcctgcgag atcggcaccg agacccatga
     2821 cagctccgga cc
//
LOCUS       DAOQOD010000277         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_19382_length_2822_cov_12.938923, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000277 DAOQOD010000000
VERSION     DAOQOD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19382_length_2822_cov_12.938923"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLZ72_15795"
     CDS             complement(<1..525)
                     /locus_tag="PLZ72_15795"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNA37813.1"
                     /translation="MTTTDTTDPWCDENDTSIAVGDWVLVDDARGNPTIQRVDALVVD
                     DFGPHLRLHNDDDPPPGHVIRLDPFPADADEPDWFHDSIGNRWVGPVGDGTIVVEIFG
                     DELALLQAAVRQAADFPVAHVELFTDTTGDHHWRAIARNGEIVATSGEGYRNASHART
                     MAERILPGVEIRDEE"
     gene            complement(549..>2822)
                     /locus_tag="PLZ72_15800"
     CDS             complement(549..>2822)
                     /locus_tag="PLZ72_15800"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HNA37814.1"
                     /translation="LDYARQGWRVIPILPGSKRPALTRWTEQATTDTATIKEWWDGHD
                     DYGVGIATGPTSGFWVLDVDDFDSFRDLEQRYEMLPDTRTSITGSGGFHFLFRWPTDG
                     RDIRNDAGRRLGPGLDVRGDGGQIVAPPSIHPNGNTYQWDAGLGDDIAEAPEWLLELV
                     CAEPTTETTERTVAAPTDRPGDRWAASTTWGELLERDGWQLHHVDRDGEHHWTRPGKE
                     LRDGTSATTGYKGSDVLKVFTSSMRAAGLDEEQTYTKLGYLASTRFDGDHSAAAAALA
                     AQGWSSPSDDPFDSPEFHASVAQLTERTLEELPDLADPDADNDDEGHWQFVDLDPILD
                     GTFDPPTPTLLPRTDGVCLLYAGRVHSLTGEPGGGKTWIALHLIADTLTAGGTAMLID
                     YEDTPASAVSRLRTLGVDDQAMRDRFAYVRPDGPLIDRQGRVAGHTMARLEALAADVV
                     VIDSIGESLAAEGFKPNDDDQVTRWFRLLPRRLARNGSAVLGLDHRAKNKDDRGLWAI
                     GSQRKLAAIDGAAYVADVKVAPTKTADGHVRLICAKDRHGTHQRDHMVADVHIRNLDG
                     GVRLHLAAPATTFRPTVLMERVSRFLEETPTSSLRGVHRGVTGKNETLTLALNSLLAE
                     GYVRCDTGGRGGQQWSSVEPFRAPDDELAHLTHPVDNPEEGDRVPPRPHRVPDAGDAV
                     SDRPRPPRPDPLRSRGRTGTRSSPLEPQETPQARPPETDDTTHDPGVPAADAAPAEIP
                     DPFALTDADLDAVDSEP"
BASE COUNT          365 a          894 c         1082 g          481 t
ORIGIN      
        1 ctcctcgtct cggatctcga cgccgggcag gatccgctcc gccatcgtcc gggcatggga
       61 ggcgttccgg tagccctccc cggacgtggc gacgatctca ccgttgcggg cgatcgctcg
      121 ccagtggtgg tcgccggtgg tgtcggtgaa tagctcgacg tgagcgacgg ggaagtcggc
      181 cgcctggcgc accgccgcct gcagcagcgc cagctcgtcg ccgaagatct cgacgacgat
      241 ggtgccgtca ccaactgggc cgacccaccg gttgccgatc gagtcgtgga accagtcggg
      301 ctcgtcggcg tcggcgggga acgggtccag cctgatgacg tggccagggg gcggatcgtc
      361 gtcgttgtgg agtcggaggt gcgggccgaa gtcgtcgacg acgagagcgt cgactcgctg
      421 gatggtcggg ttgccgcggg cgtcgtcgac gagcacccag tcacccacgg cgatcgaggt
      481 gtcgttctcg tcgcaccacg ggtcggtggt gtcggtggtg gtcatgtggt gctcctggct
      541 cgttggggtc atggctcgga atcgacagcg tcgagatcgg cgtcggtgag ggcgaacggg
      601 tcggggatct ccgcgggcgc ggcgtcggcg gccgggacgc cgggatcgtg tgtggtgtcg
      661 tcggtttccg ggggacgcgc ttgtggggtt tcctgtggct caagtgggct cgaccgcgtc
      721 cccgtccgac ccctactacg tagggggtcg ggacgcgggg gacgcggtcg atctgacacc
      781 gcgtcccctg cgtcggggac gcggtgggga cgcggtggga cgcggtcgcc ttcttcgggg
      841 ttatccacag ggtgggtgag gtgtgcgagc tcgtcgtcgg gcgcccggaa cggctcgacc
      901 gacgaccact gctggccgcc acggccaccc gtgtcgcacc tcacgtagcc ctcagcgagc
      961 agcgagttca gtgccagagt gagggtttcg ttcttgccgg tgacgccgcg gtggacacct
     1021 cgcagcgagc tcgtcggcgt ttcttcgagg aaccgactga cccgctccat cagcaccgtg
     1081 ggacggaacg tcgtcgccgg cgccgccagg tgcaggcgca cgcctccgtc gaggttgcgg
     1141 atgtggacgt cggccaccat gtggtcccgc tggtgggtgc cgtggcggtc cttggcgcag
     1201 atgagacgaa cgtgcccgtc ggcggtcttg gtgggtgcga ccttgacatc tgcgacgtag
     1261 gcggccccgt cgatcgctgc gagcttccgc tgcgacccga tggcccacag gccccggtcg
     1321 tccttgttct tggcccggtg gtcgaggccg agcacggcgg acccgttgcg ggcaagccgg
     1381 cggggcagga gtcgaaacca gcgggtcacc tggtcgtcgt cgttcggctt gaagccctca
     1441 gcggcgagcg actcgccgat cgagtcgatc acgacgacat ctgctgcgag ggcttcgagg
     1501 cgcgccatcg tgtggccggc gacccgcccc tgtcggtcga tgagcggccc atcggggcgg
     1561 acgtaggcga accggtcccg catcgcctgg tcgtcgacgc cgagggtgcg gaggcgactc
     1621 acggcggatg ctggggtgtc ctcgtagtcg atgagcatgg ccgtgccgcc agcggtgagg
     1681 gtgtcggcga tgaggtgcag tgcgatccac gtcttgccgc cgccgggttc cccggtgagc
     1741 gagtggacgc gcccggcgta gaggaggcag acgccatcgg tgcggggaag gagcgtcggt
     1801 gtgggcgggt cgaacgtgcc gtcgaggatc ggatccaggt cgacgaactg ccagtggccc
     1861 tcgtcgtcgt tgtcggcgtc cgggtcagcg aggtcgggca gctcctcgag ggtgcgctcg
     1921 gtgagctgcg cgaccgaggc atggaactcg ggcgagtcga acgggtcatc actcggggag
     1981 ctccacccct gggcggcgag agccgcagct gcggcggaat ggtcgccgtc gaaccgggtc
     2041 gacgccaggt agccgagctt cgtgtaggtc tgctcctcgt cgagcccagc ggcacgcatg
     2101 gacgaggtga acaccttgag gacgtcggag cccttgtagc ccgtcgtggc cgatgtcccg
     2161 tcccgcaact ccttgccggg gcgcgtccag tggtgctcgc cgtcgcgatc gacgtggtgg
     2221 agctgccacc cgtcccgctc gagcagttcg ccccacgtcg tggacgccgc ccagcggtcg
     2281 ccgggtcgat ccgtgggcgc ggcgaccgtg cgctcggtgg tctcggtcgt cggttcggcg
     2341 cagacgagct ccagcagcca ctccggcgcc tcagcgatgt cgtcgcccag accggcgtcc
     2401 cactggtagg tgttgccgtt cgggtggatc gacggcggag cgacgatctg tccgccgtcg
     2461 ccccgcacgt cgaggccggg gccgagccga cggccggcgt cgttgcggat gtcgcgtccg
     2521 tcggtcggcc agcggaacag gaagtggaac cctcccgagc ccgtgatact ggtgcgagtg
     2581 tcgggcagca tctcgtagcg ctgctcgagg tcgcggaagc tgtcgaagtc gtcgacgtcg
     2641 agcacccaga acccggacgt gggaccagtc gcgatgccaa cgccgtagtc gtcgtgtccg
     2701 tcccaccact ccttgatcgt cgcggtgtcg gtggtggcct gctcggtcca gcgggtgagc
     2761 gcgggtcgct tcgatccggg gaggatcggg atcacgcgcc atccctggcg ggcgtagtcg
     2821 ag
//
LOCUS       DAOQOD010000278         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_19429_length_2819_cov_10.987337, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000278 DAOQOD010000000
VERSION     DAOQOD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19429_length_2819_cov_10.987337"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLZ72_15805"
     CDS             <1..282
                     /locus_tag="PLZ72_15805"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37815.1"
                     /translation="WVAAIASGDLDGLRDACEDRLHQDIRLEHRPDARAVRDHLLGRQ
                     EVLAAWLSGSGPSVAALVPADLAPLVAGSIGGDGAVRVLDVHRPGVEAA"
     gene            266..1678
                     /locus_tag="PLZ72_15810"
     CDS             266..1678
                     /locus_tag="PLZ72_15810"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HNA37816.1"
                     /translation="MWRPPETRPQGSGIGLGSRPGATRASCRTQRGATMTDDWGFATR
                     QIHSGAEPDPTTGSRATPIYQTTAYQFRDTEHAANLFALSEIGNIYTRIMNPTQGVVE
                     ARVASLEGAPDTAVGLPGALVVASGQAAETIAILNLAEAGSHIVSSAALYGGTYNLFH
                     YTLPKLGIEVSFVEDPDDLDAWAAAVRPNTKAFYGESIGNPKNDVLDIAAVAEVAHAN
                     GVPLIVDNTIASPYLINPLAHGADIVVHSATKFLGGHGTAIAGVIVDGGSFDFSQNDK
                     FPQFTEPDPSYHGLAYWPALGPGSYIVKARVQLLRDMGAAISPFNAFLLIQGIETLSL
                     RMERHVANAQAVAEYLEAHDQVESVAYAGLASSPWHERARKYAPKGAGSVPAFIIKGG
                     REAGRRFVEGLTLHSHVANMGDVRSLVVHPASTTHSQLTDDQQRSAGVDPGLVRLSVG
                     IEDVADIIADLDRGFAAAKG"
     gene            complement(1745..2392)
                     /locus_tag="PLZ72_15815"
     CDS             complement(1745..2392)
                     /locus_tag="PLZ72_15815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNA37817.1"
                     /translation="MTHPHWPLWDIRIRTPRLELRPVREAEMADLSELIDGGIHDPAT
                     MPFLIPFTDAPNPQRTRDSYRFWMGCWAGWSIESWRLVLAAYSDGRCVGSQDVQATDF
                     PTLRTVTTGSWLGLPHQGHGLGREMRAAALQLAFEGLGAQRAATEAFEDNVASLRVTE
                     SLGYHPNGSEVVRRRDGRATVRRYVLERDDWEPRRRDDIALDGLGPECLDMFGLS"
     gene            2465..>2819
                     /locus_tag="PLZ72_15820"
     CDS             2465..>2819
                     /locus_tag="PLZ72_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37818.1"
                     /translation="MVLWRVKIRTENPELSIADVSDQRLVAAARATCRPEGLSDADEA
                     LLERLDIDPERFREAPAELPVPLICLSRRRSRDQRSMRTDGDPKATVIVAGIPSRSIG
                     SPNDELDAIDSTRPLC"
BASE COUNT          419 a         1035 c          967 g          398 t
ORIGIN      
        1 tgggtcgccg cgatcgcctc gggtgatctc gacggcctgc gcgacgcctg cgaggaccgg
       61 ctccaccagg atatccgcct ggagcaccgg cccgacgccc gggcggttcg agaccacctg
      121 ttgggccgcc aggaggtgct ggcggcgtgg ctgtcggggt cggggccgag cgtcgccgcg
      181 ctggtccccg ccgatctggc acctctggtg gccggctcga tcggcggtga cggcgcggtc
      241 cgggtgctcg acgtgcaccg cccgggtgtg gaggccgcct gaaacccgac cgcaggggtc
      301 aggaattggt ctaggatcgc gccccggtgc gacgagggcg tcgtgccgca cgcaacgagg
      361 agcgaccatg accgacgact ggggcttcgc cacccgtcag atccactccg gcgccgagcc
      421 cgacccgacc accgggtccc gcgccacccc gatctaccag acgaccgcct accagttccg
      481 cgacaccgag cacgccgcga acctcttcgc cctctcggag atcggcaaca tctacacccg
      541 catcatgaac ccgacccagg gtgtggtcga ggcccgggtc gccagcctgg agggcgctcc
      601 cgacaccgcg gtcggtcttc cgggcgcgct cgtcgtcgcc tcggggcagg cggcggagac
      661 catcgcgatc ctcaacctgg ccgaggccgg cagccacatc gtcagctcgg ccgccctcta
      721 cggcggcacc tacaacctct tccactacac gctgcccaag ctgggcatcg aggtgtcatt
      781 cgtcgaggac cccgacgacc tcgacgcatg ggccgccgcg gtgcgcccca acaccaaggc
      841 gttctacggc gagtcgatcg gcaaccccaa gaacgacgtg ctcgacatcg ccgcggtggc
      901 cgaggtcgcg cacgccaacg gcgtcccgct gatcgtcgac aacacgatcg cgtcgccgta
      961 cctgatcaac ccgctggccc acggcgccga catcgtcgtg cactccgcca ccaagttcct
     1021 gggcggccac ggcacggcga tcgcgggcgt gatcgtcgac ggcggcagct tcgacttctc
     1081 ccagaacgac aagttcccgc agttcaccga gcccgacccc agctaccacg gcctggccta
     1141 ctggccggcg ctcggcccgg gctcctacat cgtcaaggcc cgcgtgcagc tcctgcgcga
     1201 catgggagcc gccatctctc cgttcaacgc gttcctgctg atccagggca tcgagacgct
     1261 gtcgctgcgc atggagcgcc acgtcgccaa cgcccaggcg gtggcggagt acctcgaggc
     1321 ccacgaccag gtcgagtcgg tcgcctacgc cgggctggcg tcgagcccct ggcacgagcg
     1381 ggcccggaag tacgcgccga agggcgcggg gtcggtgccg gcgttcatca tcaagggcgg
     1441 ccgcgaggcc ggacgtcgct tcgtcgaggg cctcacactg cacagccacg tcgccaacat
     1501 gggcgacgtc cgcagcctgg tggtgcaccc ggcgtcgacc acccacagcc agctcaccga
     1561 cgaccagcag cgctccgccg gtgtggaccc gggcctggtc cgcctgtcgg tcggcatcga
     1621 ggacgtcgcc gacatcatcg cggacctcga ccgcggcttc gcggccgcca agggctgagt
     1681 ccgtcggtcc cgcggctccg accggagctg cgggaccgac gacggaccgg cttcgctcga
     1741 gccctcagct caggccgaac atgtcgaggc actcggggcc gagcccgtcg agagcgatgt
     1801 cgtcgcggcg tcggggttcc caatcgtctc gttcgagcac gtagcgccgc accgtcgcac
     1861 ggccgtcgcg tcggcgtacc acttcggagc cgttcgggtg gtagccgagc gactcggtca
     1921 cccgtagcga ggcgacgttg tcctcgaacg cctcggtcgc cgcccgctgc gcgcccaggc
     1981 cctcgaacgc gagctgcagg gccgcggcgc gcatctcgcg gcccagcccg tggccctggt
     2041 gggggaggcc gagccacgag ccggtcgtga ccgttcgcag cgtcgggaag tcggtcgcct
     2101 gaacgtcctg tgagccgacg cagcgaccgt cggagtaggc ggcgagcacc aggcgccacg
     2161 actcgatgct ccagccggcc cagcagccca tccagaaccg gtacgagtcg cgggtgcgct
     2221 gcgggttcgg tgcgtcggtg aacgggatca ggaacggcat ggtggccggg tcgtggatcc
     2281 cgccgtcgat cagctccgac aggtcggcca tctcggcctc gcggaccggc cgcagctcca
     2341 ggcgtggcgt ccggatcctg atgtcccaca gcggccagtg tggatgggtc atggcccgag
     2401 gatcgcccac gcagcgggtc tcgcgccatg ccgttcgtgg accatctgtc gagagggcgt
     2461 cgtgatggtg ctgtggcgcg tcaagatccg gaccgagaac cccgagttgt cgatcgctga
     2521 cgtgtcggac cagcggctgg tcgccgcggc acgggcgacg tgtcgacccg agggtctgtc
     2581 cgacgcggac gaagccctgc tcgaacggct cgacatcgac ccggagcgct tccgcgaggc
     2641 gcccgctgag ttgcccgtcc cgctgatctg cctgtcccgt cgcaggtcgc gggatcagcg
     2701 gtcgatgcga accgacggcg acccgaaggc gacggtgatc gtggccggca tccccagcag
     2761 gtcgatcggg tcgcccaacg acgagctcga cgcgatcgac agcaccaggc ccttgtgct
//
LOCUS       DAOQOD010000279         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_19701_length_2799_cov_15.580175, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000279 DAOQOD010000000
VERSION     DAOQOD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_2799_cov_15.580175"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /gene="istA"
                     /locus_tag="PLZ72_15825"
     CDS             <1..1072
                     /gene="istA"
                     /locus_tag="PLZ72_15825"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNA37819.1"
                     /translation="VVHSIRKAVCMAFREVPVFEVREVLRLWLDGRGLRSIAAVMPPD
                     RKTIRRVVEVAVRLGLDRDGGAAQLDDEFVGSVMSELAVGRPDRHGESWAAIAGEHDR
                     IAGWVDTKVPVVKMCELLARRSVVVPERTLHRYVAEHFGGTPAESTVPLADCEPGAEL
                     QVDWAKLGFIVELDTGRRRSVWALLFTSVFSRHCFVWLSHSQALDAVIAGFDAAWAFF
                     GGVFAVVIPDNMKTIVIDADATEPRLNQAFVEYAQWAGFFVDPARVRAPQDKARVERN
                     VRFLKDSFWAGESFVDLGEAQAAVEDWCRVRAGMRTHGTTGLRPIEVFVEHEAPVLAP
                     APAGRYDLPIYRDAKVARDHHV"
     gene            1148..1600
                     /locus_tag="PLZ72_15830"
     CDS             1148..1600
                     /locus_tag="PLZ72_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37820.1"
                     /translation="MKIYARGVLVKTHARVPAGHRSTDVEDLPAERTAYALRDIDRLV
                     ADASAHGAAVGEFAKVVLDHPLPWTKMRQVYKLLGLCRRYGDDRTDAACRRALDAETD
                     SVTVVGRMLERALEAETTEDDPVPDNVIRGRFARGDDHFAVTPSGVTR"
     gene            1597..2355
                     /gene="istB"
                     /locus_tag="PLZ72_15835"
     CDS             1597..2355
                     /gene="istB"
                     /locus_tag="PLZ72_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNA37821.1"
                     /translation="MNPTPIIDADLVATLRKLKLGRLADTLPERLALAKTNKLSHAEF
                     LQVLLSDEVTRRDAASADRRGRTAGLEPTMRLEHWDATTDAAFDHKLWDELCSLRFVE
                     NANNVLIMGPVGVGKTFLATALGHIAVRRRHSVHFERADRLHKRLRTAKLDNSHDAEL
                     RKLLRVDLLILDDFALGGLNQADTDDLYEIVVERHRKASTIVTSNREPIEWLALMADP
                     LLAQSAVDRLKSAAYELVVDGDSYRNRQKPTLTT"
     gene            complement(2476..2682)
                     /locus_tag="PLZ72_15840"
     CDS             complement(2476..2682)
                     /locus_tag="PLZ72_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37822.1"
                     /translation="MMTAPDRRVTGGVDTHGVIHVAAVLDSATARRLAVAEFRADTAG
                     YVALREWMSGFVTLDAVGVESTGA"
BASE COUNT          438 a          972 c          959 g          430 t
ORIGIN      
        1 ggtggttcac tcgatccgga aggcggtgtg catggcgttc agggaggtcc ctgtgttcga
       61 ggttcgtgag gtgttgaggt tgtggttgga cggaaggggg ttgcggtcga tcgcggcggt
      121 gatgccgccg gatcgcaaga cgatccggcg ggtggtcgag gtcgcggtca ggttgggcct
      181 ggaccgcgac ggtggggcag ctcagcttga cgacgagttc gtgggttcgg tgatgtcgga
      241 gttggcggtg ggccggccgg atcgtcacgg cgagtcgtgg gcagctatcg ccggcgagca
      301 cgaccggatc gctggctggg tggacacgaa ggtgccggtg gtgaagatgt gcgagctgct
      361 ggcccgccgc tcggtggtcg tgcccgagcg cacgctgcac cgctacgtcg ccgagcactt
      421 cgggggcacg ccggcggagt cgacggtgcc gttggccgac tgcgagcccg gcgccgagtt
      481 gcaggtcgac tgggccaagc tggggttcat cgtcgagttg gacacgggcc ggcggcgcag
      541 tgtgtgggcg ttgttgttca cgtcggtgtt cagccggcat tgcttcgtgt ggttgtcgca
      601 ctcccaggcg ctcgacgcgg tgatcgccgg gttcgacgcg gcgtgggcgt tcttcggtgg
      661 cgtgttcgct gtggtgatcc ccgacaacat gaagaccatc gtcatcgacg ccgacgcgac
      721 cgagccgcgg ttgaaccagg cgttcgtcga gtacgcgcag tgggccgggt tcttcgtcga
      781 tccggcgcgg gtgcgcgccc ctcaggacaa ggctcgggtc gagcgcaacg tccggttctt
      841 gaaggactcg ttctgggccg gtgagagctt cgtcgacctg ggcgaggcac aggccgccgt
      901 cgaggactgg tgccgggtgc gggccgggat gcgcacccat ggcacgaccg ggctgcggcc
      961 gatcgaggtg ttcgtcgagc acgaggcgcc ggtgctcgcc ccggcgccgg cgggccgtta
     1021 tgacctgccg atctaccgcg acgccaaggt cgcccgggac caccacgtct agatcgcgaa
     1081 ggcgctctac tcggtcccgg gcgggctcat cggccagacc gtcgccgttc gggccgacac
     1141 gaagctggtg aagatctacg cccgcggtgt gctggtcaag acccatgctc gggtcccggc
     1201 cgggcaccgc tcgaccgacg tcgaggacct cccagcggag cggaccgcgt acgcgttgcg
     1261 tgacatcgac cggctcgtcg ccgacgcgtc ggcccacggg gccgccgtcg gcgagttcgc
     1321 gaaggtggtc ctggatcacc cgttgccgtg gacgaagatg cgccaggtct acaagctgtt
     1381 ggggctgtgt cgccgctacg gcgacgaccg aaccgacgcc gcgtgtcgcc gggcgctcga
     1441 tgccgagacc gacagcgtca ccgtcgtcgg ccgcatgctg gagcgggccc tcgaagccga
     1501 gaccaccgag gacgacccgg tcccggacaa cgtgatccgg gggcggttcg cccgcggcga
     1561 cgaccacttc gccgtcacgc cctcgggggt gacccgatga accccacccc gatcatcgac
     1621 gccgacctgg tcgccacgct gcgcaagctc aagttgggcc gcctagccga caccctgccc
     1681 gaacggctcg cgctcgcgaa gaccaacaag ctgtcccacg ccgagttcct ccaggtcctg
     1741 ctctccgacg aggtcacccg acgcgacgcc gcttccgcgg accggcgggg ccgcaccgcc
     1801 gggctggagc cgaccatgcg cctcgagcac tgggacgcca ccaccgacgc cgccttcgac
     1861 cacaagctct gggacgagct gtgctccctg cgcttcgtcg agaacgccaa caacgtgttg
     1921 atcatgggac ccgtcggtgt cggcaagacg ttcctcgcga ccgcgctcgg acacatcgcc
     1981 gtgcgccgcc gccacagcgt ccacttcgaa cgcgccgacc gactccacaa gcgactacgg
     2041 acagcgaagc tcgacaacag tcacgacgcc gagctccgca agctcctgcg cgtcgacctg
     2101 ttgatcctcg acgacttcgc gctcggcggg ctcaaccaag ccgacaccga cgacctctac
     2161 gagatcgtcg tcgaacgcca ccgcaaggca tccacgatcg tgacctccaa ccgagaaccc
     2221 atcgagtggc tcgccctcat ggccgacccg ctactcgccc agtccgcggt cgaccggctc
     2281 aagtcagccg cctacgagct cgtcgtcgac ggcgacagct accgcaaccg acagaagccc
     2341 accctcacca cttgacccac cccgccaccg gcgacgagca tcacgccgcc gacgtcaaca
     2401 cccccgcggg tggtcccatg cacctggcgg aaccccggtc cgttgcatct ggcgggcgac
     2461 agccaaaccg gcacctcacg cgccggtcga ctcgacaccg accgcgtcca gggtcacgaa
     2521 gccgctcatc cactcgcgca gcgccacata gccagcggtg tcggctctga actcggccac
     2581 ggcgagccgc cgagctgtcg cgctgtcgag cacagcggcg acgtggatca ccccgtgggt
     2641 gtcgacgccg ccggtgacgc ggcggtcagg tgctgtcatc atggtcgtga tccttcggtt
     2701 cggttgcaac gggacgggat gaccggttga actcgccgac tgacccccaa cgaagacctc
     2761 gccgcatgaa caccgggacc accacccgcg gcgacttca
//
LOCUS       DAOQOD010000280         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_21078_length_2700_cov_11.732325, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000280 DAOQOD010000000
VERSION     DAOQOD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21078_length_2700_cov_11.732325"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1167
                     /gene="dapE"
                     /locus_tag="PLZ72_15845"
     CDS             94..1167
                     /gene="dapE"
                     /locus_tag="PLZ72_15845"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNA37823.1"
                     /translation="MTDGPPDLLEQTAELVAVRSESCSEGDLADRIEAEMRTLPHLDV
                     HRIGDNVVARTRLGRAQRVVLGGHTDTVPANGNETPRIDGDVLWGLGSADMKGGLAIM
                     SELARRHREPPLDVTYVFYAREEIAADRSGLLEIEAHDPSLLLGDVAVLGEPTDGAVE
                     AGCQGVIRMRVTLTGVRAHAARAWMGRNAVHRLGRLLAVLDDYEPRRPEIVGCRFHEA
                     LLATHVEGGVAGNVVPDRAAVDIVHRFAPDRDLAGAEAHVRSVLAPILEDGDTVEVLD
                     RSPAAYPALEHPLVAGLVERHGLEVRAKFGWTDVARFTAWGVPAINLGPGDATLAHTR
                     DERVHRSSLDRTFAVLTDLLTHI"
     gene            1245..2366
                     /locus_tag="PLZ72_15850"
     CDS             1245..2366
                     /locus_tag="PLZ72_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrodipicolinate N-succinyltransferase
                     N-terminal domain-containing protein"
                     /protein_id="HNA37824.1"
                     /translation="MTRDAFEQMVAAALARPEFTEPAAFGLGVATVADADGAVLDTWF
                     ASINLAENIGFAAVLHDVCGTGAPTGTVRLTAAHVDEMRARSEIFRDELHAHPNLAVL
                     EDLAGLVEWPNALPVRKVVVFTSIAALDDAPIDAHDVYLRLHLLSSRKVTPHGVNLDG
                     QFGLLSNVVWTTMGPCDVASFPAAQRVIRSAGGTVGVTSVDKFPRMIDYVVPTGVRIA
                     DAERVRLGAHLAEGTTVMHEGFVNFNAGTLGSCMVEGRISAGVVVGADTDLGGGCSIM
                     GTLSGGGKEVIEVGERCLIGANAGIGISLGDGCIVEAGLYVTAGTVVTLPDGTRHKAK
                     DLSGSPDLLFRRNSISGAVEVLPREGTWQEGLNEALHAN"
     gene            complement(2418..>2700)
                     /locus_tag="PLZ72_15855"
     CDS             complement(2418..>2700)
                     /locus_tag="PLZ72_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNA37825.1"
                     /translation="GDGELFMLRVRGDSMIDAGILDGDFVVARVQDKVSNGDIIVAGI
                     PGEEATVKTFTRSGSRVTLLPANERLEPMEFPAADVSVFGRVVTVLRKL"
BASE COUNT          393 a          943 c          955 g          409 t
ORIGIN      
        1 ccgccgccgc ctccgacctg agtccgtccc ggcagcgctc gtcggggcga tcccggtcgt
       61 cacccggtgc ggtcgccgtg tagacactcg gccgtgaccg acggaccacc cgacctgctg
      121 gagcagaccg ccgagctcgt cgcggtccgc tccgagagct gctccgaggg ggacctggcc
      181 gatcggatcg aggccgagat gcggacgctc ccgcacctgg acgtgcaccg catcggcgac
      241 aatgtcgtcg cccgcacccg actgggccgg gcccagcggg tggtgctcgg cggccacacc
      301 gacacggtgc cggcgaacgg caacgagacg ccccggatcg acggcgacgt cctgtggggc
      361 ctgggctccg ccgacatgaa gggcggtctc gccatcatgt cggagctggc ccggcgtcat
      421 cgtgagccgc cgctcgacgt gacctacgtg ttctacgcga gggaggagat cgcggcggac
      481 cggtcgggtc tgctcgagat cgaggcgcac gatccgtcgc tcctcctcgg tgacgtcgcc
      541 gtcctcggcg agccgaccga cggagcggtc gaggccggat gccagggggt gatccggatg
      601 cgggtgaccc tcaccggggt ccgggcgcat gcggcccggg cgtggatggg gcgcaacgcc
      661 gtgcaccgcc tcggccgact gctggccgtc ctcgacgact acgagccccg ccgacccgag
      721 atcgtcggtt gccggttcca cgaggcgctg ctcgccaccc acgtcgaggg cggggtggcc
      781 ggcaacgtcg tgccggaccg ggcggcggtc gacatcgtcc accggttcgc cccggaccgc
      841 gacctggccg gcgctgaggc ccacgtccgg tcggtgctcg ccccgatcct cgaggacggc
      901 gacaccgtcg aggtgctcga ccgatcgccg gccgcctacc cggcgttgga gcacccgctc
      961 gtcgccggtc tggtcgagcg acacggcctc gaggtccggg ccaagttcgg gtggaccgac
     1021 gtcgcccgct tcaccgcctg gggcgtgccg gcgatcaacc tgggtccagg ggacgcgacg
     1081 ctggcccaca ccagggacga gcgggtccac cgatccagcc tcgatcgcac cttcgccgtc
     1141 ctgaccgacc tcctcaccca catatagggg tccgcaccga cataccttgt cgttatcgcg
     1201 cccccagaaa tccggagaac cctgtgtccg accccaaccg accgatgacc cgtgatgcct
     1261 tcgagcagat ggtggctgcc gcgctggccc gtccggagtt caccgaaccc gcggcgttcg
     1321 ggctcggcgt ggccacggtc gccgatgccg atggcgcggt cctcgacacg tggttcgcct
     1381 cgatcaacct ggccgagaac atcgggttcg cggccgtgct gcacgacgtg tgcgggacgg
     1441 gtgctccgac cggaacggtg cggctgaccg cggcgcacgt cgacgagatg cgagcccggt
     1501 cggagatctt ccgtgacgag ctgcatgcgc acccgaacct ggccgtgctc gaggacctgg
     1561 ccggcctggt cgagtggccc aacgcgctgc cggttcgcaa agtcgtcgtg ttcacttcga
     1621 tcgctgcgct cgacgacgct ccgatcgatg cccacgacgt ctacctacga ctccatctgc
     1681 tgtcgtcccg gaaggtgaca ccccacggcg tgaacctcga cggccagttc ggcctgctgt
     1741 ccaacgtggt ctggacgacg atgggtccgt gcgacgtcgc ctcgttcccg gcggcgcaac
     1801 gcgtgatccg cagcgctggg gggaccgtgg gcgtcaccag cgtcgacaag ttcccgcgga
     1861 tgatcgacta cgtggtgccc accggcgtgc gcatcgctga tgccgagcgg gtgcgtctgg
     1921 gtgcgcatct ggccgagggc accaccgtga tgcacgaggg attcgtcaac ttcaacgccg
     1981 gcaccctcgg ctcgtgcatg gtcgagggcc ggatctccgc cggggtcgtc gtcggcgccg
     2041 acaccgacct ggggggcggg tgctcgatca tgggcaccct gtccggcggt ggcaaggagg
     2101 tcatcgaggt gggggagcgc tgcctgatcg gagcgaacgc gggtatcggc atctcgctcg
     2161 gcgacgggtg catcgtcgaa gcgggcctct acgtcaccgc ggggacggtc gtgaccctcc
     2221 cggacggaac tcggcacaag gccaaggacc tgagcggatc acccgatctg ctgttccgtc
     2281 ggaactcgat cagcggagcg gtggaggtgc tgccgcggga gggcacgtgg caggaaggcc
     2341 tcaacgaggc cctgcacgcc aactgatcga ggcctgacga ccgaccttcc gaccgtcgag
     2401 cgttggagcc ggtgggctca gagcttgcgg aggacggtga cgacccggcc gaacacggac
     2461 acgtcggccg cggggaactc catcggttcg agccgttcgt tcgcgggcag gagcgtgacc
     2521 ctggaccctg aacgggtgaa ggtcttgacc gtcgcctctt ctccggggat tccggcgacg
     2581 atgatgtctc cgttggagac cttgtcctgg acccgggcga cgacgaagtc gccgtcgagg
     2641 attccggcgt cgatcatgga gtcgccccgg acccgcagca tgaacagctc gccgtcgccg
//
LOCUS       DAOQOD010000281         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_21429_length_2677_cov_14.931732, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000281 DAOQOD010000000
VERSION     DAOQOD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21429_length_2677_cov_14.931732"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..814
                     /locus_tag="PLZ72_15860"
     CDS             299..814
                     /locus_tag="PLZ72_15860"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNA37826.1"
                     /translation="MVEPFRRRRQSRPGPRVERGAAIVEFALVVPLFLLLVMGIIDFS
                     NAFNDYNSVRQGVREGARQIVVADWSTDGCTSGTSSVRAACVTRARVGLNTADTKVRI
                     ELPSTYAPGEQVTVCVMYPFRSLTGLFSPVLNGGVARSKLTMRIESIDETDPIATYED
                     TPLTGQGWSWC"
     gene            871..2427
                     /locus_tag="PLZ72_15865"
     CDS             871..2427
                     /locus_tag="PLZ72_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNA37827.1"
                     /translation="MLAALLLVLMLTMAAIAVDLANGRQQRRQAQATADAAALAAAQD
                     LPDPAVVVATAKTYVQQNFGVPASDWVGCQDPEALANRPDDAVGDQCISIDEAFTRVR
                     VRIPDKQVPTVFAGVIGVDHMAVSADAVAEAKLKRDDRIIPATVAASSGSGNLCIENG
                     GNNVECASRSSGNFGSFDARRLSLFKPTSSDQANSLRINYSMGVDHALTIYGTGSPKV
                     CDVNLQSPCAMTNASSTLDANHLIPYTGNVVPPLTDGVIENATIATDEGTNLFCGRLK
                     RPDLTDTNLADTDPEDCNHWSSTPGPGPAITVLGDKVNGRHVFTWMKPAFRTVFFPGA
                     SAATPSTSGTWAAGDGRFNCFLRSYRFDYGGSYSSGHTPQTEFFIDPSKPIDPSTGQG
                     TEFTLAQAKAYLTGTCGLDSSTVDSKLASLTDSSSFWPMFDNAMVTDPRFGMIPVVKN
                     FGSGGSTAMQVVRFWAIYMYRLYPNNGNTKLAGIDAWVFEPALIETDSGVADLQFGYR
                     TNQPVVRLVQ"
BASE COUNT          432 a          940 c          865 g          440 t
ORIGIN      
        1 aggcggcggc ggacaacgtg ctggtgctct ccgacggtcg gatcctgctg tcggggttcg
       61 tgggcaactc agtcgtcgtg ttcagccccg acggaacagg tggctggatc cggaccgtga
      121 ccgccgtcgg cagctgacct ctccccgctt tgtcccgcgc aggcgcggtg atcgccgcgt
      181 ctgtgcggga caaagcggct cggtggcgta ataggtcgtt cgacccaatc tcgatccacc
      241 gtgtaacggc agatccctgc tgagcgctct ccctgacgag tcagtgagga gatctccgtt
      301 ggtcgagccg ttccgccgca ggcgccaatc ccgtccgggt ccaagggtcg agcgaggcgc
      361 ggccatcgtc gagttcgcgt tggtcgtccc gctgttcctc ctgctcgtca tggggatcat
      421 cgacttctcg aacgccttca acgactacaa ctcggttcgt cagggagtcc gagaaggtgc
      481 ccgccagatc gtggtggccg actggagcac cgacggctgc acgagcggca cctcgtcggt
      541 ccgggcggcc tgcgtgaccc gggcccgcgt cggtctgaac accgccgaca ccaaggtgcg
      601 gatcgagctg ccttcgacct acgccccggg tgagcaggtg acggtctgcg tcatgtaccc
      661 gttccggtcg ctcacggggc tgttctctcc cgttctgaac ggtggtgtcg cccgatcgaa
      721 gttgacaatg cgcatcgaat cgatcgacga gaccgatccg atcgccacct acgaggacac
      781 cccgctgacg ggccaggggt ggtcgtggtg ctgacgcggt cgacgaggct cgcagcctgc
      841 agacgccgtg acgaacgcgg tgcgttcctc gtgctcgcgg cgctgctgct ggtgctcatg
      901 ctcaccatgg ccgcgatcgc ggtcgacctg gccaacggcc gccagcaacg ccgtcaggcc
      961 caggccaccg cggatgccgc cgcgctcgcc gcggcacagg accttccgga cccggctgtc
     1021 gttgtcgcga ccgccaagac ctacgtgcag cagaacttcg gggtgcccgc ctcggactgg
     1081 gtcgggtgcc aggatcccga ggccctggcc aaccgacccg acgacgcggt cggtgatcag
     1141 tgcatctcga tcgacgaggc gttcacccgg gtgcgggtga ggatccccga caagcaggtg
     1201 ccgacggtgt tcgccggtgt catcggggtg gatcacatgg ccgtgtcggc cgacgccgtc
     1261 gccgaagcca agctcaagcg cgacgaccgg atcatccccg cgacggtggc ggcgtcatcg
     1321 ggcagcggga acctctgcat cgagaacggc ggcaacaacg tcgagtgcgc ctctcgcagc
     1381 agcggcaact tcgggtcctt cgacgcccga cggctgtcgt tgttcaagcc gacatcgagc
     1441 gaccaggcca actcgttgcg gatcaactac tcgatgggtg tcgaccacgc cctcaccatc
     1501 tacgggaccg gctcgcccaa ggtgtgcgac gtcaacctcc agtcgccgtg cgccatgacg
     1561 aacgcgtcgt cgaccctgga cgccaaccac ctcatcccct acacgggcaa cgtcgtccct
     1621 cccctgaccg acggagtgat cgagaacgcg accatcgcca ccgacgaggg gaccaacctg
     1681 ttctgcgggc ggctcaagcg ccccgacctg accgacacga acctggccga caccgatccg
     1741 gaggactgca accattggtc ctcgacgccg ggacccgggc ccgcgatcac cgtcctcggc
     1801 gacaaggtca acggtcgcca cgtcttcacc tggatgaagc ccgcgttccg cacggtgttc
     1861 ttccccggag cgtcggccgc gacgccgagc accagcggga cgtgggcggc cggtgacggt
     1921 cggttcaact gcttcctgcg cagctaccgg ttcgactacg gcggctcgta ctcgtcgggt
     1981 cacaccccgc agaccgagtt cttcatcgat ccgagcaagc cgatcgaccc gtcgaccggg
     2041 cagggcaccg agttcaccct cgcccaggcc aaggcctacc tcaccgggac ctgtggcctc
     2101 gactcgtcga ccgtcgactc caagctggcc agcctcaccg actccagctc cttctggccg
     2161 atgttcgaca acgccatggt caccgacccg cgcttcggga tgatccccgt cgtcaagaac
     2221 ttcggcagcg gagggagcac cgccatgcag gtcgtccggt tctgggcgat ctacatgtac
     2281 cggttgtacc cgaacaacgg gaacaccaag ctggccggga tcgacgcctg ggtgttcgag
     2341 ccggcgctga tcgagaccga ctccggcgtc gccgacctcc agttcggcta ccggaccaac
     2401 cagccggtgg tgcggctcgt ccagtgatcg gaaccacgtc cgaggcgcgc cgcttcgagg
     2461 ccgctcggtc gccgttgcga tgcgcttccc acgtcggcat cggaggaagc ggccacgccg
     2521 gccgataccc tcggggtcgc cgacgtcatc cggtcggtgg acggacgatg gcgacgcacg
     2581 acgagttcga acgacttctc gatggtgtcc gcgacgggag tgaggccgcg gcgtcggagc
     2641 tgttccggcg gacccaaccc cgtctgctgc gctatct
//
LOCUS       DAOQOD010000282         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_22208_length_2627_cov_7.549767, whole genome shotgun sequence.
ACCESSION   DAOQOD010000282 DAOQOD010000000
VERSION     DAOQOD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22208_length_2627_cov_7.549767"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PLZ72_15870"
     CDS             <1..1139
                     /locus_tag="PLZ72_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37828.1"
                     /translation="CQMSEDERWVVSWPADGGELTIRDLRSGDVRRVEGTTTGAVVLG
                     HGTSVLAVQTDGDGSRGVVIDATTGKVTGRTDRYDDLQVMPVEPDSTGFVALASSGEQ
                     AAVVADRQLLWVGTDARTQVIDRGLLVLPVQSTSTVTYLRFGNQEAGSDSIRRWDSGT
                     GARTTLLSGAVGLAAVGPDELIATRDTDTAVEVFRSNSLGELEQVAEVPGSATGGSSV
                     GRVLTLGSTSYLDLSVGAETSLVRVDLHGDHSDVPVQGWAQMLLEGVDGDGTVLVSGR
                     RTVESTSESIGVVAPNGDGFEERATAGATGLNLIHEGTIYLTTRDGEDGELTVRTVRS
                     SGETDGETLYTGYQLAGATWPTDNGATQSTLRSRAALIAQQQSQ"
     gene            complement(1240..>2627)
                     /gene="purH"
                     /locus_tag="PLZ72_15875"
     CDS             complement(1240..>2627)
                     /gene="purH"
                     /locus_tag="PLZ72_15875"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNA37829.1"
                     /translation="TEQVTGFADMLGHRVVTLHPAIHGGILADRDDPSHVADMDTHGI
                     VGIDLVAVNLYPFSSEPSVELIDIGGPTMVRGAAKNHAHVTVLVDPADYEPVLDELRA
                     TGEVSAITRRRLARDAFAHTAAYDSAIVEWFDDGGPGEQPPLPEHHAPLPRTLHLSAS
                     LAQPLRYGENPHQVGARYSFSGDSCWDRATLHGGKEMSYLNVYDADAAWRLVHSLGDE
                     PAAVIIKHANPCGAAVADDITTAYVRAHECDPTSAFGGIVAVNRPVTLDMAKALAPVF
                     TEVVIAPGYEDEALMVLLEKRNLRVLTAPAPTVPALDVRSVDGGLLVQTRDLVTVDRS
                     AWQVVTERHPSEQEWADLELAWRLVARVTSNTIVLVKDGQAVGVGAGQQSRVDAARIA
                     GTKADGRASGGAGASDAFFPFRDGLDAVADQGVTAVIQPGGSIRDEEVVAAADERGLA
                     MVLTGERHFKH"
BASE COUNT          364 a          928 c          975 g          360 t
ORIGIN      
        1 tgtgccagat gagcgaggac gagcgctggg tcgtgtcctg gccggccgac ggcggcgagc
       61 tcaccatccg agacctgcgg tccggcgacg tgcggcgcgt cgagggaacc accaccggcg
      121 cggtcgtgct cggccacggg acctcggtgc tggcggtgca gaccgacggc gacggctccc
      181 ggggcgtggt catcgacgcc accaccggca aggtcaccgg tcgcaccgac cgctacgacg
      241 acctgcaggt catgccggtg gagcccgact cgaccgggtt cgtggcgctg gcgtcctcgg
      301 gcgagcaggc ggcggtggtt gcggatcgcc agctcctgtg ggtcggcacc gacgcccgga
      361 cccaggtcat cgaccggggc ctgctggtgc tgccggtgca gtcgacctcg acggtgacct
      421 acctgcgctt cggcaaccag gaggccggct cggactcgat ccgccggtgg gactcgggca
      481 ccggggcgcg cacgacgctg ctgtcgggag cggtcggcct ggccgccgtc ggccccgacg
      541 agctcatcgc cacccgcgac accgacaccg ccgtcgaggt cttccgctcc aactccctgg
      601 gcgagctcga gcaggtggcc gaggtgcccg gatcggcgac cggcggcagc tcggtggggc
      661 gggtcctcac gctggggtcc acctcgtacc tggacctgag cgtcggggcc gagacgtcgc
      721 tggtgcgcgt cgacctccac ggtgaccaca gcgacgtgcc ggtgcagggg tgggcgcaga
      781 tgcttctcga gggcgtcgac ggcgacggca ccgtgctcgt cagcggccgc aggaccgtcg
      841 agtcgacctc ggagtcgatc ggggtggtcg cgccgaacgg ggacgggttc gaggagcgtg
      901 ccacggccgg ggcgaccggg ctgaacctga tccacgaggg cacgatctac ctgacgaccc
      961 gcgacggcga ggacggcgag ctgaccgtgc gcaccgtgcg gtcctcgggc gagaccgacg
     1021 gcgagacgct gtacaccggg taccagctgg cgggggcgac gtggccgacc gacaacggcg
     1081 ccacgcagtc gaccctgcgc tcccgcgccg ccctcatcgc ccaacagcaa tcccagtgat
     1141 ataggggtcc gcaacgacac accttgtcgc aatcccgacc tcagaaatcc gggatcgcgc
     1201 gccgcgaccg ctgcacgccg gcttcgggac cgacgcaggt cagtgcttga agtggcgctc
     1261 gccggtgagg accatcgcca ggccccgctc gtcggccgcg gcgacgacct cctcgtcacg
     1321 gatcgacccg ccaggctgga tcaccgcggt cacaccctgg tcggcgaccg cgtcgagccc
     1381 gtcgcggaac gggaagaagg cgtcgctcgc ccccgctccc ccgctcgccc gaccgtcggc
     1441 cttggtgccg gcgatgcgcg ccgcgtcgac ccggctctgc tgaccggcgc cgaccccgac
     1501 ggcctgaccg tccttgacca gcacgatcgt gttcgacgtg acccgggcca ccagccgcca
     1561 cgccaactcg aggtccgccc actcctgctc gctcgggtga cgttcggtga cgacctgcca
     1621 cgccgagcgg tcgacggtga ccaggtcacg ggtctgcacc agcaggccgc cgtcgacgct
     1681 gcggacgtcg agtgcgggca ccgtcggcgc cggcgcggtc agcacccgca ggttccgctt
     1741 ctcgagcagc accatcagcg cctcgtcctc gtagccgggg gcgatgacca cctcggtgaa
     1801 caccggcgcc agcgccttgg ccatgtcgag ggtgaccggc cggttgaccg ccacgatgcc
     1861 cccgaaggcc gaggtggggt cgcactcatg cgcccggacg taggcggtgg tgatgtcgtc
     1921 ggccaccgcc gcgccgcacg ggttggcgtg cttgatgatg accgcggcgg gttcgtcgcc
     1981 gagggagtga accagccgcc aggccgcatc ggcgtcgtac acgttgaggt acgacatctc
     2041 cttgccgccg tggagggtgg cgcggtccca gcagctgtcg ccggagaacg agtagcgggc
     2101 gccgacctgg tgggggttct cgccgtagcg caacggctgg gccagcgacg ccgacaggtg
     2161 cagggtgcga ggcagcgggg cgtggtgctc gggcaggggc ggctgctcgc cgggcccgcc
     2221 gtcgtcgaac cactcgacga tcgcgctgtc gtacgcggcg gtgtgcgcga acgcgtcccg
     2281 ggccaggcgg cggcgggtga tggccgacac ctcgccggtg gccctcagct cgtcgagcac
     2341 gggctcgtag tcggcggggt cgacgagcac cgtgacgtgc gcgtggttct tggccgcgcc
     2401 gcgcaccatg gtcggcccgc cgatgtcgat gagctcgacc gacggctcgg agctgaacgg
     2461 gtagaggttg accgccacca ggtcgatgcc gacgatgccg tgggtgtcca tgtcggcgac
     2521 gtgggacggg tcgtcacgat cggccaggat ccctccgtgg atggccgggt gcagcgtgac
     2581 cacccggtgc ccgagcatgt cggcgaaacc cgtgacctgc tcggtgg
//
LOCUS       DAOQOD010000283         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_24475_length_2496_cov_12.172061, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000283 DAOQOD010000000
VERSION     DAOQOD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24475_length_2496_cov_12.172061"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1206)
                     /locus_tag="PLZ72_15880"
     CDS             complement(25..1206)
                     /locus_tag="PLZ72_15880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNA37830.1"
                     /translation="MSGRGDGSEAEAPSGDGPGPLADLRVIDLATVIAGPGCARYLAD
                     FGADVIKVERPPGGDGVRAMGWTDPTDGLSLWWKIVGRNKRTIALDLKDPDDLAAVRR
                     LIDSADVLVENFRPGTLERLGLDPDVLHRTNPRLVVTRVTGFGQTGPYKDRPGFATQA
                     EALSGFAAINGEPDGQPLLPPIALTDEVAALVAAFATMVAVHSGVGQVVDVNLIESML
                     QLMGPLVPLYGLRGEVQERLGAGIPYTVPRNTYRTSDGRWVAVSTSAESVAERVMDLI
                     GLGDDPRFGDFSGRVAHRDLIDERMAEWMAARTMEQVLAEFEAAHAAVAPVYDMADIA
                     ADPHFRARGSIVEVDGVPMPEVVARLSATPGRVRWAGRPLDADGEAIRREVDPDGSAA
                     G"
     gene            complement(1203..2240)
                     /locus_tag="PLZ72_15885"
     CDS             complement(1203..2240)
                     /locus_tag="PLZ72_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNA37831.1"
                     /translation="MANHPDDLLPEPMPAEVMTVADDEVIVFRGATSVRATELRPDTD
                     VTVDGVEVHTLPRRGELLARVVTANDVHFGEVLCGHIDGADWETYSVEPGQPPYPETM
                     NAAVVADMAAWEPDAVVVKGDVTSNGTDDEVDRFLEVYGGAFGDRLTWVRGNHESYHH
                     NQRGAWPTQEVTLPGVTLAVLDTSRDGRINGDLDAEQLDWLDELGQRADRPVMVFGHH
                     PVWNPAEEARHDSTFGLVPDATEALAETFARRPRLLGYFAGHTHRNKRIGLPGVGDVP
                     FVEVGTVKDFPGSWAEYRIHDGMVLQVHRRVTDPVALEWSERTRGMFAGTYSGYARGR
                     RSDRCFAMVAR"
BASE COUNT          342 a          911 c          904 g          339 t
ORIGIN      
        1 ggccggcgcc gtcgacgtgg ccgctcagcc ggcggcggac ccgtcggggt cgacctcgcg
       61 gcggatcgcc tcgccgtccg cgtcgagcgg acgacccgcc caacgcaccc gacccggcgt
      121 ggccgacagc cgggccacga cctcgggcat cggaaccccg tcgacctcga cgatcgagcc
      181 gcgtgcacgg aagtgcgggt cggcggcgat gtcggccatg tcgtagaccg gcgccaccgc
      241 cgcatgggcc gcctcgaact ctgcgagcac ctgttccatc gtccgggccg ccatccactc
      301 cgccatgcgc tcgtcgatca ggtcccggtg cgccacccgg cccgagaagt ccccgaagcg
      361 ggggtcgtcg ccgagtccga tgaggtccat gacccgttcg gcgacggact cggcgctggt
      421 cgacaccgcc acccaccgac cgtcggaggt ccggtacgtg ttgcggggga ccgtgtaggg
      481 gatgcccgcg ccgagacgct cctgcacctc gccgcgcaag ccgtacagcg gcacgagcgg
      541 gcccatgagc tgaagcatcg actcgatgag gttgacgtcg acgacctgtc cgacccccga
      601 gtgcaccgcg accatggtgg cgaacgcggc gaccagcgcc gccacctcgt cggtgagcgc
      661 aatgggcggc agcagcggtt gaccgtcggg ctcgccgttg atcgcggcga accccgacag
      721 cgcctcggcc tgggtcgcga accccgggcg gtccttgtac gggccggtct ggccgaagcc
      781 ggtcacccgg gtgaccacca gacgcgggtt ggtgcggtgc agcacgtcgg gatccaggcc
      841 gaggcgctcg agggtgccgg gccggaagtt ctcgaccagc acgtccgcgg agtcgatcag
      901 ccgacgcacg gcggcgaggt catcggggtc cttgaggtcg agggcgatcg tccgcttgtt
      961 gcgcccgacg atcttccacc acagcgacag cccgtcggtc gggtcggtcc accccatggc
     1021 cctgaccccg tcgcccccgg gcgggcgttc gaccttgatc acgtccgcac cgaagtccgc
     1081 gaggtagcgg gcgcatcccg gcccggcgat caccgtcgcc aggtcgatca cccgcaggtc
     1141 cgccagcgga ccgggcccgt caccggaggg ggcctcggcc tcggagccgt caccacgacc
     1201 gctcatcggg ccaccatcgc gaagcagcgg tcggaccgtc tgccccgggc gtatcccgag
     1261 taggtcccgg cgaacatccc gcgggtgcgc tcgctccact ccagggcgac cgggtcggtc
     1321 acccggcggt ggacctgcag caccatcccg tcgtggatcc ggtactcggc ccacgacccc
     1381 gggaagtcct tgaccgtgcc gacctcgacg aaggggacgt cgcccacgcc gggcagaccg
     1441 atgcgcttgt tgcggtgggt gtgcccggcg aagtagccga gcagacgggg gcggcgggcg
     1501 aacgtctctg ccaacgcctc ggtggcgtcg gggaccaggc cgaaggtcga gtcgtggcgg
     1561 gcctcctcgg ccgggttcca caccgggtgg tgaccgaaca ccatgacggg ccggtcggcg
     1621 cgttggccga gctcgtcgag ccagtcgagc tgctccgcgt ccaggtcgcc gttgatgcga
     1681 ccgtcccgcg acgtgtcgag cacggccagg gtgacgccgg gcagggtcac ctcctgggtg
     1741 ggccatgcgc cccgctggtt gtggtggtag ctctcgtggt tgccccgcac ccaggtgagc
     1801 cggtccccga acgctccgcc gtagacctcg aggaaccggt cgacctcgtc gtcggtgccg
     1861 ttcgaggtga cgtcgccctt gaccacgacg gcgtcgggct cccacgccgc catgtcggcg
     1921 accaccgccg cgttcatcgt ctcgggatac gggggttgtc ccggctccac cgagtaggtc
     1981 tcccagtccg cgccgtcgat gtggccgcac aggacctcgc cgaagtggac gtcgttcgcg
     2041 gtgaccaccc gggccagcag ctcgccgcgt cggggcaggg tgtgcacctc gaccccgtcg
     2101 acggtgacgt cggtgtcggg gcggagctcg gtggcgcgca ccgacgtcgc tccccggaac
     2161 acgatcacct cgtcatccgc gacggtcatc acctcggccg gcatcggttc gggcagcagg
     2221 tcgtcggggt ggttcgccac gtcgcccgag gatacggcgt cggccgaggg gtaccgtcgg
     2281 gcacggtcaa cggcgcccgg acacgaccgg cgtcggtggc atcgggcgct actggctgag
     2341 gagagacgcc gatcagccgt cgacgagccg ggcgacccga cggtcccagc ggcgaagggc
     2401 ccgctcatcg ggctcggccg gcgacgcggc gccgagtcga gcggcgatcg cgcaccgggc
     2461 gagccacggc accatggcgc gggcgagtcc cgggtc
//
LOCUS       DAOQOD010000284         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_25270_length_2456_cov_12.626822, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000284 DAOQOD010000000
VERSION     DAOQOD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25270_length_2456_cov_12.626822"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLZ72_15890"
     CDS             <1..514
                     /locus_tag="PLZ72_15890"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNA37832.1"
                     /translation="RADPETIPGHRGSDGTVERSPNRAPWLWRVAEDGGAVVNTQSDP
                     IDDEMVGSIDPAAVRASLEALRAELLDSAPDIDAEVFRTRSDVSDGSGETEHLVVAEQ
                     KDMTARVDALTERAVAEIDEALARLDAGTYGRCTDCGRAIPAERLEAVPAAATCMDCA
                     ATRSRGRGLV"
     gene            630..1259
                     /locus_tag="PLZ72_15895"
     CDS             630..1259
                     /locus_tag="PLZ72_15895"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNA37833.1"
                     /translation="MAPPRTRLLVLGVVRIFGPVHGYDVRRELLSWRMQDWMNIQPGS
                     IYSALKTLEKDGLVETVDDPEGGAAGGGGVPKRSYRVTAEGEKVFQAELRAAWWRVER
                     AAEPLIPALCLMPFMPRAELIAAIGARVQQLGADGEQLRFQRALIHDGATGADGGIPE
                     HVRELLDFATARIEAEIAWSRTFLRRLEAGDYWFAEDGPPPGHGSPPVP"
     gene            1393..2394
                     /locus_tag="PLZ72_15900"
     CDS             1393..2394
                     /locus_tag="PLZ72_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA37834.1"
                     /translation="MTDTTTPSRTKMIEVRGLTQTFRTRTGPVDAVKGIDFEVRDGEI
                     LGLLGPNGAGKTTTLRMLCTLLKPTAGSAVVAGIDVSGDAREVRRRIGYVAQVGAVPA
                     PGTVVGEEIVVQARLQGMSKADAQARMRALLPSLSLEGFEDRALSEMSGGQRRRFDVA
                     LGLVHEPKLVFLDEPTTGLDPQSRANLWDYIRMLRDDRGVTIVLTTHYLDEADALADR
                     IVVMGHGSIVADDTPDALKAQVSGDVISVAVDGDLRAAERVAADVLSPRTSTIEDLGG
                     EGERLVLTVERGDTAVPVLLRALDEASLPLTAVEVRRPTLDDVFLAMTGRSLREEGS"
BASE COUNT          357 a          879 c          861 g          359 t
ORIGIN      
        1 ccgggccgat ccggaaacca tcccggggca ccgggggtcc gacggtacag tggagcgttc
       61 accgaaccgc gccccgtggc tgtggcgcgt cgccgaggac ggaggtgccg tcgtgaacac
      121 ccagagcgat cccatcgacg acgagatggt cggttccatc gacccggccg ccgtccgagc
      181 gtcgctcgag gcgctgcgtg ccgagctgct cgactccgcc cccgacatcg acgccgaggt
      241 cttccggacc cgttccgacg tgtcggacgg atcgggtgag accgaacacc tggtcgtggc
      301 cgagcagaag gacatgaccg cccgggtcga cgccctgacc gagcgggctg tcgccgagat
      361 cgacgaggct ctcgcacggc tcgacgccgg cacctacggg cgctgcaccg actgcgggcg
      421 cgccatcccg gccgagcggt tggaggcggt gcccgccgcg gccacgtgca tggactgcgc
      481 cgccacccgc tcccgcggcc gcggcctggt ttgaccgccg ccccctcacc caccccaccc
      541 gttctgttcg ttggatcaca cctctgacgt tgtgatccaa cgaacagaac ggggtgttcg
      601 gggtggtcaa ggttgactag ggtgaggtga tggcgccccc gcgcacccgc ctcctggtcc
      661 tcggcgtggt ccggatcttc gggccggtcc acggctacga cgtccggcgt gagctgctca
      721 gctggcggat gcaggactgg atgaacatcc aacccggctc gatctactcc gctctcaaga
      781 cgctcgagaa ggacgggctg gtcgagaccg tcgacgaccc cgaaggcggc gcggcgggtg
      841 ggggcggggt gcccaagcgg tcctaccggg tgaccgccga gggcgagaag gtgttccagg
      901 ccgagctgcg tgccgcgtgg tggcgggtgg agcgggcggc cgagccgctg atcccggctc
      961 tgtgcctcat gccgttcatg ccccgtgccg agctcatcgc cgcgatcggg gcccgggtcc
     1021 agcaactcgg tgccgacggc gagcagctgc ggttccagcg ggcgttgatc cacgacgggg
     1081 cgaccggcgc cgacggcggc atccccgaac acgtccgtga gctcctcgac ttcgccacgg
     1141 cccgcatcga ggccgagatc gcctggagtc ggacgttcct gcgccgtctc gaagcggggg
     1201 actactggtt cgccgaggac ggcccgcccc cgggtcacgg ctccccgccg gttccgtagt
     1261 ccgcccgctc gtcgcgcagc ccggccgttc tgtagtgcga gatccaggtg ttcgtgtgga
     1321 tctcgcacta cagaacgctc gaaataatag tcaaggttga ctacttcggg gtggtcgtgt
     1381 cagggtgacc gtgtgacaga caccaccacg ccctcccgga cgaagatgat cgaggtgcgg
     1441 ggtctcaccc agaccttccg gacccgcacc ggacccgtcg atgcggtcaa ggggatcgac
     1501 ttcgaggtcc gtgacggcga gatcctcggt ctgctcggcc ccaacggcgc cggcaagacc
     1561 acaacgcttc ggatgctgtg caccctgctc aagccgaccg ccggatcggc ggtggtcgcc
     1621 ggcatcgacg tctccgggga tgcccgggag gtccggcggc gcatcggcta cgtcgcccag
     1681 gtcggagcgg tgccggcgcc gggcacggtc gtcggcgagg agatcgtcgt gcaggcccgc
     1741 ctccagggca tgtccaaggc cgacgcccag gcccgcatgc gggccctgct cccatcgctc
     1801 agcctcgagg ggttcgagga ccgggccctg tcggagatgt cgggtgggca gcgccgccgc
     1861 ttcgacgtgg ccctcggcct ggtgcacgaa cccaagctcg tgttcctcga cgagcccacc
     1921 accgggctgg acccgcagag ccgcgccaac ctctgggact acatccgcat gctgcgcgac
     1981 gaccgtggcg tcacgatcgt gttgaccacc cactacctcg acgaggccga cgccctggcg
     2041 gaccgcatcg tcgtcatggg ccacggctcg atcgtcgcgg acgatacgcc ggatgccctc
     2101 aaggcccagg tgtccggcga cgtgatcagc gtcgcggtcg acggcgacct gcgagccgcc
     2161 gagcgggtcg ccgccgacgt gctgtcgccg cggacgagca cgatcgagga cctcgggggt
     2221 gagggggagc ggctggtgct gaccgtggaa cggggcgaca ccgcggtgcc ggtgctgctg
     2281 cgggcgctcg acgaggcatc gctgccgctg accgcggtcg aggttcgccg gccgaccctc
     2341 gacgacgtct tcctggccat gaccggacga tccctccggg aggagggctc atgatcgctc
     2401 gccccagcca tcccgccgac cccagccatc ccgccgaccc cagccatccc gccgac
//
LOCUS       DAOQOD010000285         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_26199_length_2411_cov_9.224109, whole genome shotgun sequence.
ACCESSION   DAOQOD010000285 DAOQOD010000000
VERSION     DAOQOD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26199_length_2411_cov_9.224109"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1073)
                     /gene="rsmH"
                     /locus_tag="PLZ72_15905"
     CDS             complement(48..1073)
                     /gene="rsmH"
                     /locus_tag="PLZ72_15905"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNA37835.1"
                     /translation="MADDPAAPDPPFEHAPVMVELIVELLRDVPDGIFVDATLGGGGH
                     AEAVLDAHPGLRLLGLDRDDVAIAAATRRLARFGDRVRIVRSGFDQLERIVGEAVGGS
                     GSATDPSDPSHLDPYPATEGVSAVLFDLGVSSPQLDEMERGFSYRSGAPLDMRMDRRQ
                     AFSAVDVVNDYSFPQLARVIETYGEERFARRIARAIVDARPIRDTGHLAEVVRSAIPA
                     ATRRHGGHPAKRTFQAIRIEVNRELDQLAEALDGAMAVLAPGGRMVVMSYHSLEDRMV
                     KQTLRDAETGGCTCPPALPCVCGAEPTVRLLKRGAWKADEAEIALNRRAESVRVRAAE
                     RLIPESA"
     gene            complement(1302..1736)
                     /locus_tag="PLZ72_15910"
     CDS             complement(1302..1736)
                     /locus_tag="PLZ72_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37836.1"
                     /translation="MDFYGTFEHTIDGKGRLVMPSMFRSAFEGGGVAAMVEDYAALFT
                     TDEWGRYRRKLEDSGQFTRDDLQWIFSLTSTFTPDAQNRISLPTKLRDHVGLGKDVTL
                     IGSVRFVAVFPRQEWARREAAVAAQAATGPSIADKFRSLEFL"
     gene            complement(1959..>2411)
                     /locus_tag="PLZ72_15915"
     CDS             complement(1959..>2411)
                     /locus_tag="PLZ72_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37837.1"
                     /translation="LVVPAGDVLADDDVVRAAAWVGELWVRALGLGPFEDSGRLVRHD
                     GRWSDRDLGARACFAAVGPGELVLDGRKVLGISQRRTRAGARFQCLAYRRWDPESLLG
                     LLAGAGVGGSEWGDGAGPDALRSALVERAGAVVPDGWDVVERLLPVLP"
BASE COUNT          348 a          865 c          885 g          313 t
ORIGIN      
        1 cggtgcggct gcggtcgcgc tacgggcgcg cggcgatcgg gctgcggtca cgccgactcc
       61 gggatgagcc gctcagcggc ccgcacccgc acgctctcgg cccgacggtt gagggcgatc
      121 tcggcctcgt cggccttcca cgccccccgc ttgaggaggc ggacggtcgg ttcggcgccg
      181 cacacgcagg gcagcgcggg cggacacgtg cacccgccgg tctcggcgtc gcgcagggtc
      241 tgcttgacca tgcggtcctc gagggagtgg tacgacatga cgaccatgcg gccgccgggg
      301 gcgaggaccg ccatggcgcc gtcgagcgcc tcggccagct ggtcgagctc gcggttgacc
      361 tcgatgcgga tggcctggaa ggtgcgcttg gccggatggc cgccgtggcg tcgggtcgcc
      421 gcggggatgg cggagcggac cacctcggcc aggtgaccgg tgtcgcggat ggggcgggcg
      481 tcgacgatcg cccgggcgat ccgacgggcg aagcgctcct cgccgtaggt ctcgatcacc
      541 ctcgccagct gcgggaacga gtagtcgttg acgacgtcga cggcggagaa cgcctggcgg
      601 cggtccatcc tcatgtcgag cggcgcgccg gagcggtagc tgaagccccg ctccatctcg
      661 tcgagctgcg gggaggacac tccgaggtcg aacagcacgg cactcactcc ttcggtcgcg
      721 gggtacgggt ccaggtggga cgggtctgac gggtctgttg cggagccgga gccgccgacg
      781 gcctcgccga cgatccgctc gagctggtcg aacccgctgc gcacgatgcg gacgcggtcg
      841 ccgaagcggg ccaggcgacg ggtggccgcg gcgatggcga cgtcgtcgcg gtcgaggccg
      901 agcaggcgca gcccggggtg ggcgtcgagg acggcctcgg cgtggccgcc gccgccgagg
      961 gtggcgtcga cgaagatgcc gtcggggacg tcgcggagca gttcgacgat gagctcgacc
     1021 atgaccgggg cgtgctcgaa cggcgggtcc ggggcggcgg ggtcatcggc cacgggctcc
     1081 tgctccgcac agacgggtcc ggtccgggag ctgtccccgg tggtcgatcc cccgacgacg
     1141 cgacgaccgc cgggatgtct ccccggacgg cacggaatgg cgcggacgga gctctgggcc
     1201 aaccctcacg tcggccaggg gatcggccac cgggcacagc ggcggaccag ggttcaggat
     1261 cgggatcggg atcgggttcg gggactgagc cggggccgac atcacaggaa ctccagcgac
     1321 cggaacttgt cggcgatgct cggcccggtc gctgcctgcg ccgcgaccgc cgcttcccgt
     1381 cgggcccact cctgccgggg gaacaccgcg acgaagcgca ccgatccgat cagggtcacg
     1441 tccttgccca gcccgacgtg gtcgcgcagc ttggtcggca gcgagatgcg gttctgtgcg
     1501 tcaggggtga acgtcgaggt gagcgagaag atccactgca ggtcgtcccg ggtgaactgg
     1561 ccgctgtcct cgagcttgcg ccggtagcgg ccccactcgt cggtggtgaa cagcgccgcg
     1621 tagtcctcga ccatcgcggc gacgccgccg ccttcgaacg cggaccggaa catcgacggc
     1681 atgaccagac ggcccttgcc gtcgatcgtg tgctcgaagg ttccgtagaa gtccacgccc
     1741 gctccacttg gggctcgtca gctgggaagg tgagccctgg tgccccccat tcctcccttt
     1801 cgagccaccc taaaccactc tcctccacca cacaacgttt acagcggtgt agttcgcgcg
     1861 cttcgcgcga atcccccacc ccaccccgcg ttctgacgac aagtcacgac acaggcggta
     1921 ctcagatgtc gtcagaaact cggtggcggg gtggggcgtc agggaaggac ggggagcagg
     1981 cgctccacca cgtcccagcc gtcggggacg accgcccccg cgcgctccac cagggcggag
     2041 cgcagcgcgt cgggtcccgc cccgtcgccc cactccgacc cacccacacc cgcacccgcc
     2101 agcagcccga gcagcgactc gggatcccac cggcggtagg cgaggcactg gaaccgcgcc
     2161 ccggcccggg tccgccgctg cgagatcccc agcaccttgc gaccgtcgag caccagctcg
     2221 ccgggtccga ccgcggcgaa gcaggcccgg gcccccaggt cccggtcgct ccagcgcccg
     2281 tcgtggcgga ccagccgacc ggagtcctcg aaggggccga gcccgagcgc ccggacccag
     2341 agctcaccga cccaggcggc cgcccgcacg acgtcgtcat cggccaggac gtcgccggcc
     2401 gggacgacga g
//
LOCUS       DAOQOD010000286         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_26200_length_2411_cov_8.664261, whole genome shotgun sequence.
ACCESSION   DAOQOD010000286 DAOQOD010000000
VERSION     DAOQOD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26200_length_2411_cov_8.664261"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..558)
                     /locus_tag="PLZ72_15920"
     CDS             complement(115..558)
                     /locus_tag="PLZ72_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNA37838.1"
                     /translation="MCDHCGCREYPPIAELSADHVEILALAEDLATATRLRTPIDAAG
                     RDRLRSLLEVHAAKEEVGLYPLLIAQMGEHADTYSHLEEEHRDIARAIDAGCFEHHAF
                     YALQRHIEEEEEILFSSALFWFDGDTWDDLEAVHRGLVSSPTDVG"
     gene            726..1829
                     /locus_tag="PLZ72_15925"
     CDS             726..1829
                     /locus_tag="PLZ72_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37839.1"
                     /translation="MDRTSATAPTGPPPRRSRRRTPEVFGVRVDVRAAVWSTGAVLVV
                     LVGVYLGSGGLQWFDAALAGYLIGVLLGVFATVYRYLIWIQRPPTARLNQRGWESFRR
                     SGRVGRNAVGLVGLVGTNLLAQGFIRRRSTVRWAAHQLVFWGCILAAAVTFPLTFGWV
                     HFESVGQDGSEYKAYLFGVGTGSFQAMSVVGFITFHLLNIAAFLVIAGVLVFLYRRLR
                     DPGALAVEREGDFLALAGLFAVSVTGLFLTASSLWLEGRYYSFLNNLHALTVILGLLY
                     IPFGKLFHIFQRPANLGIHLYRDAGREGAQQECRRCGRPYASQMQVQDIKEVLPMVGF
                     DYSSPDGHWQETCPACRRAGVTIAQTGRVGGFG"
     gene            1829..>2411
                     /locus_tag="PLZ72_15930"
     CDS             1829..>2411
                     /locus_tag="PLZ72_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNA37840.1"
                     /translation="MAQLPVTEEQLIERFGPHLNREPPGGWNAGLDVEREVETHCCFC
                     GQQCGIKLKVADNQVIGFEPWYDFPFNQGKLCPKGVKRYLQGAHPDRLLDPLERDPSA
                     ESGFRPIAWDAAIDRVATEIRRIQDAYGPDSFAVLSGVSLTNEKSYLMGKFARLGIGT
                     ANLDYNGRLCMVSAGVANKKALGMDRAPNPWSDI"
BASE COUNT          358 a          833 c          817 g          403 t
ORIGIN      
        1 gcgtcggtga tgccgacggt gacgagcaac aacatcaacg ccccggtgat catgatcggc
       61 gagaaggccg ccgacatgat ccgatccgac gcgtaggccc ggtccggggc ggcctcagcc
      121 gacgtcggtg ggggagctca ccaatccccg gtgcacggcc tcgaggtcgt cccaggtgtc
      181 gccgtcgaac cagaagagcg cggagctgaa caggatctcc tcctcttctt cgatgtgacg
      241 ctgcagggcg tagaacgcgt ggtgctcgaa gcaacccgcg tcgatcgccc gggcgatgtc
      301 gcggtgctcc tcctcgaggt gggagtacgt gtcggcgtgc tcacccatct gggcgatgag
      361 gagcggatac agcccgacct cctccttggc cgcgtgtacc tcgagcaggg aacggagtcg
      421 gtccctgccg gcggcgtcga tcggggtacg gagcctggtc gccgtcgcca gatcctcggc
      481 cagtgccagg atctccacgt ggtccgccga cagttcggcg atgggcgggt actcacgaca
      541 tccgcagtga tcgcacaccg gatcctccgt cccggccggg ggacgacgct ccgcccaccg
      601 acaggtattt ctctaccagg cgacagacat aataccgggc gatccggtcg tcgggaccat
      661 ggggacggct ctgttacggt ctcggcggtc ggcactcgat gatcgtcggg aggagcaggc
      721 aggcgatgga ccgaacctct gcaacggccc cgaccggccc acctccccgc cgcagccgac
      781 ggcggacccc cgaggtgttc ggcgtacggg tcgacgtccg cgccgcggtg tggtcgaccg
      841 gcgccgtcct cgtcgtcctc gtcggggtgt acctcggcag cggagggctg cagtggttcg
      901 atgccgctct tgccggctac ctgatcggcg tgctcctcgg ggtcttcgcg acggtgtacc
      961 ggtacctgat ctggatccag cggccgccga ccgcccggct gaaccagcgg ggctgggagt
     1021 cgttccgacg atcgggtcgc gtggggcgca acgccgtcgg tctggtcgga ctggtcggca
     1081 ccaacctgtt ggcccagggg ttcatacgcc gtcgatccac cgtccgctgg gcggcgcatc
     1141 agctcgtctt ctggggttgc atcctggccg ccgcggtcac gttcccgctc accttcggct
     1201 gggtgcactt cgagtcggtc ggccaggacg gcagcgagta caaggcctac ctgttcggcg
     1261 tcggcaccgg gtccttccag gcgatgagcg tcgtcggctt catcacgttc cacctgctca
     1321 acatcgccgc gttcctcgtg atcgcgggag tgctggtctt cctctaccgc cgcctccggg
     1381 accccggcgc cctggcggtc gagcgagagg gcgacttcct ggccctcgcg gggctgttcg
     1441 cggtgtcggt caccggactg ttcctcaccg cgtcgagcct gtggctggag ggtcgctact
     1501 acagcttcct caacaacctg cacgccctga ccgtcatcct cggactgctg tacatcccgt
     1561 tcgggaagct gttccacatc ttccaacggc cggcgaacct gggcatccac ctctaccggg
     1621 acgccggtcg ggagggcgcc cagcaggagt gccgtcgatg cggtcgcccg tacgcgtcgc
     1681 agatgcaggt ccaggacatc aaggaggtcc tcccgatggt cggcttcgac tactcgtctc
     1741 ccgacggcca ctggcaggag acctgcccgg cctgccgccg ggccggcgtc accatcgccc
     1801 agaccggtcg ggtggggggc ttcggctgat ggcgcagctc ccggtcaccg aggaacagct
     1861 gatcgagcgg ttcggcccgc acctcaaccg cgaacccccg ggcggctgga acgccggctt
     1921 ggacgtcgag cgcgaggtgg agacccactg ctgcttctgc ggccagcagt gcgggatcaa
     1981 gctcaaggtg gccgacaacc aggtgatcgg cttcgagccc tggtacgact tcccgttcaa
     2041 ccagggcaag ctctgcccca agggcgtgaa gcgctacctc cagggcgcac atccggaccg
     2101 gctgctcgat ccgttggagc gcgacccgag cgcagagtcc gggttccggc cgatcgcctg
     2161 ggacgcggcg atcgaccggg tggcgaccga gatccgccgc atccaggacg cctacggccc
     2221 cgactcgttc gccgtgctgt cgggcgtgtc gttgaccaac gagaagtcgt acctgatggg
     2281 caagttcgcc cgcctgggca tcggcacggc caacctcgac tacaacgggc ggctctgcat
     2341 ggtgtccgcg ggggtcgcca acaagaaggc gctcggcatg gaccgcgccc ccaacccgtg
     2401 gagcgacatc c
//
LOCUS       DAOQOD010000287         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_26836_length_2384_cov_14.126234, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000287 DAOQOD010000000
VERSION     DAOQOD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26836_length_2384_cov_14.126234"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLZ72_15935"
     CDS             <1..248
                     /locus_tag="PLZ72_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37841.1"
                     /translation="AELQDMSVESKMLEVEQASINVEAQARLSEIRSKLGIADAAAPA
                     TTEPATTEPAATEPASGEPATSESTPAESGTEPSPGT"
     gene            complement(294..2090)
                     /locus_tag="PLZ72_15940"
     CDS             complement(294..2090)
                     /locus_tag="PLZ72_15940"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNA37842.1"
                     /translation="MPTPDHDALRERYRIERDKRIRPDGNDQYLQPTGRYAHLLDDPY
                     TTPDERDPVRVDVDVAIIGGGFAGLCVGARLRERGVQDLRIIEGGGDVGGAWYWNRYP
                     GAMCDTAAMIYLPLLEETGYLPSAKYVPAPEIFAHARRIAETFDLYRDALLSTQVRSL
                     NWDDGASRWIVTTDRGDEIRARFVTMGTGPLHRPKLPGIPGIETFDGHAFHTSRWDYD
                     YTGGDAAGAPMTGLADKRVGIIGTGATAVQAIPHLATSAAELHVFQRTPSSVDVRANR
                     TFGPDDFAELGPGWQREWLINFATLQTGGFTDVDLVKDGWTDISQRIRDRIVDMMAVP
                     GTELTPELMQQAYEDSDDEKMEEIRARIDSIVADPATADALKPWYRQLCKRPCFHDEY
                     LQAFNEPGVRLIDTDGRGVSAIDATGVWVDDDHYELDCLIFASGFEVGTDQARRSGFE
                     TTGRDGVTLTDAWAEGMVSLHGMHVRGFPNLFITGLAQGANLVSNITHNLTEAGSTAA
                     AVIGHALDTGAEQVEVTEAAQDAWMQLLDGNPRSFMGNAECTPGYYNNEGGPIGRTQR
                     RGMSGYPDGPVAFFDHIDRWRRSGEFAGLEFS"
     gene            complement(2161..>2384)
                     /locus_tag="PLZ72_15945"
     CDS             complement(2161..>2384)
                     /locus_tag="PLZ72_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37843.1"
                     /translation="LALLCRHHHGVIHRTGWHMTLNPHTDGASRDGADGNQTSFFTIT
                     TPDGARLPTHHDRPPRQDQPPRQSRPAPA"
BASE COUNT          312 a          786 c          905 g          381 t
ORIGIN      
        1 tggccgagct gcaggacatg tcggtcgagt cgaagatgct cgaggtcgag caggcatcga
       61 tcaacgtcga ggcccaggcc cggctgtcgg agatccgctc caagctcggc atcgccgacg
      121 ccgcggcccc ggccaccacc gaacccgcga cgaccgaacc cgcggcgacc gaacccgcct
      181 ccggcgagcc ggccacctcc gagtcgacgc ccgccgagtc cggcacggag ccctctccgg
      241 gcacctgagt cagcccgaca cgcctccgcc ccactccggg tccgggcagg cgttcagctg
      301 aactcgaggc cggcgaactc gccggagcgt cgccaccggt cgatgtggtc gaagaaggcg
      361 accgggccgt cggggtaccc gctcatcccc cggcgctgcg tgcggccgat cggcccgccc
      421 tcgttgttgt agtagccggg ggtgcactcg gcgttgccca tgaacgaccg ggggttgccg
      481 tcgagcagct gcatccaggc gtcctgagcg gcctcggtca cctcgacctg ctcggcaccg
      541 gtgtcgaggg cgtggccgat gaccgcggcc gcggtcgacc cggcctcggt gaggttgtgg
      601 gtgatgttcg acaccaggtt ggcgccctgg gccagcccgg tgatgaacag gttggggaac
      661 ccccggacgt gcattccgtg cagcgacacc atgccctcgg cccacgcgtc ggtgagggtg
      721 accccgtcgc gaccggtggt ctcgaacccg ctgcggcggg cctggtcggt gccgacctcg
      781 aagccggagg cgaagatcag gcagtcgagc tcgtagtggt cgtcgtcgac ccacacgccg
      841 gtggcgtcga tggcgctgac gccgcgaccg tcggtgtcga tcaggcgcac gccgggctcg
      901 ttgaacgcct ggaggtactc gtcgtggaag cagggccgct tgcacagctg gcggtaccac
      961 ggcttgagcg cgtcggcggt ggcggggtcg gccacgatcg agtcgatgcg ggcgcggatc
     1021 tcctccatct tctcgtcgtc gctgtcctcg taggcctgct gcatcagctc gggggtcagc
     1081 tccgtgcccg ggaccgccat catgtcgacg atccggtccc ggatccgctg ggagatgtcg
     1141 gtccagccgt ccttcaccag gtcgacgtcg gtgaagccgc cggtctggag ggtggcgaag
     1201 ttgatcaacc actccctctg ccagcccgga ccgagctcgg cgaagtcgtc gggaccgaag
     1261 gtccggttgg cccgcacgtc gaccgacgac ggcgtgcgct ggaacacgtg cagctccgca
     1321 gccgaggtgg cgaggtgcgg gatcgcctgg accgccgtcg ccccggtgcc gatgatgccg
     1381 acccgcttgt cggccaaccc ggtcatcggc gcgcccgccg cgtcgccgcc ggtgtagtcg
     1441 tagtcccagc ggctggtgtg gaaggcgtgg ccgtcgaatg tctcgatgcc ggggatgccg
     1501 gggagcttgg gccggtgcaa cggcccggtc cccatggtga cgaaccgggc gcggatctca
     1561 tctccccggt cggtggtcac gatccagcgg gacgcgccat cgtcccagtt cagtgagcgc
     1621 acctgggtgg acaggagggc gtcgcggtag aggtcgaagg tctcggcgat gcggcgggcg
     1681 tgggcgaaga tctccggggc cggcacgtac ttggccgacg gcaggtaccc ggtctcctcg
     1741 agcagcggca ggtaaatcat cgccgcggtg tcgcacatgg cgccggggta gcggttccag
     1801 taccacgcgc cgccgacgtc tccgccgccc tcgatgatgc gcaggtcctg cacgcctcgc
     1861 tcgcgcagcc gcgcgccgac gcacaggccg gcgaacccgc caccgatgat ggccacgtcg
     1921 acgtcgacac ggaccgggtc ccgctcgtca ggggtggtgt atggatcgtc gagcaggtgg
     1981 gcgtagcgcc cggtgggctg gaggtactgg tcgttgccgt cgggccggat ccgcttgtcg
     2041 cgctcgatcc ggtaccgctc ccgcagggcg tcgtgatcgg gggtgggcat ggacggaccg
     2101 tacccgcgcc ggggcgcggg tggtcgctgg gcgtgggttg gtccggtgcc ggtcccgcgc
     2161 tcaggcgggt gcgggtctgg attgtcgggg tggctggtcc tgtcggggtg gccggtcgtg
     2221 gtgggtgggg agtcgggcgc cgtcgggggt ggtgatggtg aagaagctgg tctgattgcc
     2281 gtcagcgccg tctcgtgagg ctccgtcggt atgggggttg agggtcatgt gccatccggt
     2341 gcggtggatg acgccgtggt ggtggcggca cagcaacgcg aggt
//
LOCUS       DAOQOD010000288         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_27314_length_2362_cov_11.667100, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000288 DAOQOD010000000
VERSION     DAOQOD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27314_length_2362_cov_11.667100"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLZ72_15950"
     CDS             complement(<1..447)
                     /locus_tag="PLZ72_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNA37844.1"
                     /translation="MILVDAAGVTAVRPGRPLFEDLSVTVSTGDRLGIVGLNGCGKST
                     LLSILAGTTDPEAGTVRRGRDVRVSLLGQDPALQGPLVRDDLFGVGPTDAADPGSASS
                     AAPGAERWEAAAVADRLGIGDLLDADVGSLSGGQAKRVALARALVTP"
     gene            complement(470..>2362)
                     /locus_tag="PLZ72_15955"
     CDS             complement(470..>2362)
                     /locus_tag="PLZ72_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNA37845.1"
                     /translation="RGPARSYPLGMRGRAKVGGFVIAVVAAAVVGACTAPPPPDGPVP
                     SPAGTSGRTCAPAAGRSCALPYPSDQWTVADRSTPTGLRLDLPADLIPASVQSALGPG
                     AHLEDVQAGADGFSAVGPVVFELDRPVDAASLPADGGDVVRVFDRATGEPVPLRVELT
                     LDSIRQGAPDTIVMAWPQVRWEPGRTYVARLADGLSGPQGAPGRPAGMDDTAGVGGSF
                     ISSVRADLARIEGDRWDTTLEATRFTVRSTRTATATMDRIVAAARAADHPVRNLTVGP
                     APLTPSGSAVVRGEVQLSDFRDADGVVRADRPPVGTWVPFVMVLPARPAGPDGAPVVV
                     YGHGLTAAKETLLVVAEANAVHGMATIGIDVPNHGDRQAGEGGYLLDITTPGTLGRLV
                     SMPLQGEVDTVSLFRAITAHMADLDLAGPGATGWNAPDGRPDLDTARLLYQGTSMGGV
                     LGAASITQIPELDGAYLQVPGSGIADILYHSQLWPLFAGVVPWGASAGDAAALRGLAT
                     LLLDPAENSYLVDRLRDGAPPLFVQYGVGDGVVPNFSTERLAVLADLPLVGPEISPLS
                     LDLRRSGADAVPADGRGIAQVYPLHSSTEMSAFMAHLSFAEPAADVVLDQWLTNRSRA
                     LGLGAG"
BASE COUNT          338 a          858 c          890 g          276 t
ORIGIN      
        1 cggtgtcacc agggcacggg ccagtgccac ccgcttggcc tgcccgcccg acagggagcc
       61 gacgtcggcg tcgagcaggt cgccgatgcc caggcggtcg gccaccgcgg cggcctccca
      121 ccgctcggcg ccgggagccg ccgacgacgc agacccgggg tcggccgcat cggtggggcc
      181 gaccccgaag aggtcgtcac ggaccagggg cccctgcaac gccgggtcct gacccaggag
      241 cgacacccgg acgtcacgac cccgccggac cgtccccgct tcggggtcgg tcgttccggc
      301 gaggatcgac agaagggtcg acttcccgca gccgttcagt cccactatcc cgagtcggtc
      361 cccggtggag accgtgaccg acaggtcctc gaacagcgga cggcccggcc ggacggcggt
      421 gacgccggcg gcgtcgacga ggatcacgcg gtggtggccg agcgggccgt cacccggcgc
      481 ccagacccag cgcccggctg cggttcgtca accactggtc gagcacgacg tcggcagccg
      541 gttcggcgaa gctcaggtgg gccatgaacg ccgacatctc cgtcgagctg tgcagggggt
      601 acacctgcgc gatgccacgg ccgtcggcgg ggacggcgtc agccccgctc cgccgcaggt
      661 cgaggctcag cggtgagatc tcgggcccga ccaacggcag gtccgcgagc acggcgagcc
      721 gctcggtgct gaagttgggg accacgccgt cgccgacgcc gtactgcacg aacagcggag
      781 gcgcgccgtc acgaagtcgg tccaccagat aggagttctc cgccgggtcg agcagcagcg
      841 tggccaaccc tcgcagcgca gcggcgtcac ccgcgctggc tccccacggc acgacgccgg
      901 cgaacagcgg ccacagctgc gagtggtaga ggatgtcggc gatgccgctg ccgggcacct
      961 gcaggtaggc gccgtcgagc tcggggatct gggtgatcga cgccgcgccc agcacgccgc
     1021 ccatcgacgt cccctggtag agcagccggg cggtgtcgag atcgggtcga ccgtccggcg
     1081 cgttccaccc ggtggccccc ggaccggcca ggtccagatc ggccatgtga gcggtgatcg
     1141 cccggaacag cgacaccgtg tccacctcgc cctgaagggg catggagacc agccgcccga
     1201 gggtgcccgg ggtggtgatg tcgagcaggt agcccccctc gccggcctgg cggtcgccgt
     1261 ggttggggac gtcgatgccg atcgtggcca tgccgtgcac ggcgttggcc tcggccacga
     1321 ccagcagggt ctccttggct gcggtgagcc cgtggccgta gaccacgacg ggcgctccgt
     1381 cgggaccggc cgggcgggcg ggcaggacca tgacgaacgg gacccaggtc ccgaccggcg
     1441 ggcgatcggc tcggaccact ccgtcagcgt cgcggaagtc cgacagctgg acctcgccgc
     1501 gcaccacggc ggaccccgac ggggtcaacg gcgccgggcc gacggtcagg ttgcggaccg
     1561 ggtgatcagc ggcccgggcc gccgccacga tgcgatccat cgtggcggtg gccgtgcggg
     1621 tgctgcgcac ggtaaagcgg gtcgcctcca gcgtggtgtc ccagcggtca ccctcgatgc
     1681 gggccagatc ggcacgcacc gaggagatga agctgccgcc gacgcccgcg gtgtcgtcca
     1741 tgccggccgg tcgtccgggt gcgccctgcg gaccgctcag cccgtcggcc agccgggcca
     1801 cgtaggtgcg gcccggctcc catcggacct gcggccaggc catcacgatc gtgtcgggtg
     1861 cgccctgtcg gatcgagtcg agggtcagct cgacacgcag cggaaccggc tcgccggtgg
     1921 cgcggtcgaa gacccggacg acgtcaccgc cgtccgcggg gagcgatgcg gcgtcgacgg
     1981 gtcggtcgag ttcgaacacc accggaccga cggccgagaa cccgtcggca ccggcctgca
     2041 cgtcctcgag gtgggcgccc gggccgaggg cggactgcac cgacgcgggg atgagatcgg
     2101 cgggcaggtc caaccggagt ccggtcggcg tggaccgatc ggcgacggtc cactggtcgc
     2161 tcgggtacgg gagggcgcag gatcggcccg cggccggagc gcacgtccgg ccgctggtgc
     2221 ccgccggcga cggcaccgga ccgtcgggcg ggggtggggc cgtgcacgcc ccgaccacag
     2281 cggccgccac gaccgcgatc acgaatccgc cgacctttgc tctcccgcgc atgcccagag
     2341 ggtaggaccg cgccggccca cg
//
LOCUS       DAOQOD010000289         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Microthrixaceae bacterium isolate AMR_MDS_0479
            NODE_38338_length_1990_cov_11.850129, whole genome shotgun
            sequence.
ACCESSION   DAOQOD010000289 DAOQOD010000000
VERSION     DAOQOD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810111
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microthrixaceae bacterium (wastewater metagenome)
  ORGANISM  Microthrixaceae bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia;
            Acidimicrobiales; Microthrixaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,194
            CDSs (total)                      :: 3,147
            Genes (coding)                    :: 3,139
            CDSs (with protein)               :: 3,139
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Microthrixaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38338_length_1990_cov_11.850129"
                     /isolate="AMR_MDS_0479"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2497627"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..456)
                     /locus_tag="PLZ72_15960"
     CDS             complement(4..456)
                     /locus_tag="PLZ72_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNA37846.1"
                     /translation="MTPRKDTHSQIRTLPIFAGCTEAELDEIDRLADEVHISAGRTLM
                     RQGDLGQEFAVIISGEADVVKDGQVVASLGPGAYFGEVALLDAVTRTASIVARTDMVL
                     EVIDRRGFNTLLDDLPRLSRSMLLGTAKRLAELEDECTHLRHQLATRN"
     gene            642..1031
                     /locus_tag="PLZ72_15965"
     CDS             642..1031
                     /locus_tag="PLZ72_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HNA37847.1"
                     /translation="MVKLIALLRRRPGLGPEEFRAHWRDVHGPLIASTPELARHVVRY
                     EQHPRHRPDALSGTDGVDGVAVQWFDSIDDFVAFISEPAYQELIAPDERRFLDIDAIE
                     FVITEEPTVVIDGPGAVSPGPATGERS"
     gene            1028..1870
                     /locus_tag="PLZ72_15970"
     CDS             1028..1870
                     /locus_tag="PLZ72_15970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNA37848.1"
                     /translation="MSVGLEGRGIVVTGGGSGIGAATCRLLALKGATVGVLDRNADAA
                     RAVAEEIGGLALACDVGASDQVDAAIARADAELGGLTGLVNNAGLGNLKPLEDYSDRD
                     VELIWRVNFFGSYACLRAAAPFLRVAAGRRREPSAVVNVASVSGVRPTRGEAPYSAAK
                     AATVALTSSAALEWAPTVRVNCVSPGFIHTPLNDMLVSDEAARSGVEDRTPMGRVGSA
                     SETAGLIAFLLSDESSYITGQNVVIDGGTMLTNAQMDPVLGPLLELFSGGDPDADGTD
                     PGHN"
BASE COUNT          285 a          720 c          715 g          270 t
ORIGIN      
        1 gggtcagttg cgggtggcga gctggtggcg gaggtgggtg cactcgtcct cgagctcggc
       61 caggcgcttg gcggtgccga gcagcatcga gcgcgacagc cggggcaggt cgtcgagcag
      121 ggtgttgaag ccgcggcggt cgatgacctc gagcaccatg tcggttcggg cgacgatcga
      181 ggcggtgcgc gtcaccgcgt cgagcagcgc cacctcgccg aagtaggcgc cgggcccgag
      241 cgacgcgacg acctgaccgt ccttgaccac gtcggcctcg ccgctgatga tcaccgcgaa
      301 ctcctggccc aggtcgccct ggcgcatcag cgtccgaccc gcgctgatgt ggacctcgtc
      361 ggccaaccgg tcgatctcgt cgagctcggc ctcggtgcat ccggcgaaga tcgggagggt
      421 gcggatctgc gaatgggtgt ccttgcgcgg tgtcacgcgg gcgactctaa cgccgggccc
      481 cgagccgggg gccgtgaagg cggatccgcc cgcccccgtc cggtcgcgtc gatcgcgtag
      541 cttgcggacc ggacgggggt caccggtcgg caacccaccg acggaacgcc cacccgggac
      601 catcgatctc gtccaccgac tcggaccaca gggggccacc gatggtgaag ttgatcgcgc
      661 tgctccggcg gcgaccgggg ctcgggcccg aggagttccg cgcccactgg cgagacgtcc
      721 acggccccct gatcgcgtcg accccggagc tggcccgcca cgtcgtccgc tacgagcagc
      781 acccccggca ccgcccggac gccctgtcgg gcaccgacgg cgtcgacggc gtggcggtcc
      841 agtggttcga cagcatcgac gacttcgtcg ccttcatctc cgaacccgcc taccaggagc
      901 tgatcgcccc cgacgagaga cgtttcctcg acatcgacgc catcgagttc gtgatcaccg
      961 aggagccgac ggtcgtgatc gacgggccgg gagcggtatc gccgggaccg gcgacggggg
     1021 agcggtcatg agcgtcgggc tcgagggccg cgggatcgtc gtcaccggag gcgggtccgg
     1081 catcggcgcc gccacctgcc gactcctcgc cctcaagggc gcgacggtcg gcgtgctcga
     1141 ccgcaacgcg gatgccgccc gcgccgtggc cgaggagatc ggggggctgg ccctggcctg
     1201 cgacgtcggc gccagcgacc aggtcgacgc cgcgatcgcc cgggccgacg ccgagctcgg
     1261 cggtctgacc gggctggtca acaacgccgg gctgggcaac ctcaagccgc tcgaggacta
     1321 cagcgaccgc gacgtcgagc tgatctggcg ggtcaacttc ttcggcagct acgcctgcct
     1381 gcgtgccgcg gccccgttcc tgcgggtcgc ggccgggcgc cggcgcgagc cgtccgcggt
     1441 cgtcaacgtc gccagcgtct ctggggtgcg tcccacccgg ggcgaggcgc cctactcggc
     1501 ggccaaggcc gcgaccgtcg cgctcacgtc gtccgccgcg ctcgagtggg cgccgacggt
     1561 cagggtcaac tgcgtgtcgc ccgggttcat ccacaccccc ctcaacgaca tgctggtcag
     1621 cgacgaggcg gcccggtccg gcgtcgagga ccgcacgccg atgggccggg tcggctccgc
     1681 gtccgagaca gcggggctga tcgcgttcct gctctccgac gagtcctcct acatcaccgg
     1741 ccagaacgtg gtgatcgacg gcgggaccat gctgaccaac gcccagatgg acccggtgct
     1801 gggcccgctg ctcgagctgt tctcgggcgg cgaccccgac gccgacggga ccgaccccgg
     1861 gcacaactag aacgcgttcc agttctggcc tagcgtggca gaatggtcgt cgggggcagg
     1921 acccccgacc cgactcccga gggggacgac ctcatggcag cacagtccga cacagcagca
     1981 caggccgaca
//
